LOCUS       DATMTI010000001         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_212096, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000001 DATMTI010000000
VERSION     DATMTI010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212096"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VN039_00005"
     CDS             complement(<1..340)
                     /locus_tag="VN039_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44403.1"
                     /translation="MTSILDFKDVEIPEDNAPPVAMPEGASSYDPDFPGSTEEAPYGY
                     KADGTPYKRRPNAGAGRPKGSGSKGVGKVGESQARAAAQLLGQLNSLVGMSLAAFGMP
                     MTAGSIMEANV"
     gene            complement(494..766)
                     /locus_tag="VN039_00010"
     CDS             complement(494..766)
                     /locus_tag="VN039_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44404.1"
                     /translation="MSDMHVESDAVPDVVPDVTPETPVEEAHHHAEDSGLSERVDRIE
                     ASVSELSDKVTGLLTPDLPGGDDEEEEERDETPVSVPWTHRGFGGH"
     gene            complement(770..967)
                     /locus_tag="VN039_00015"
     CDS             complement(770..967)
                     /locus_tag="VN039_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44405.1"
                     /translation="MSLDTPIAPLSDMSLGDRITALESEVASMRTTLDGILLQITAIS
                     EAITPTIESLKNNPMFKMMGL"
     gene            complement(1271..1783)
                     /locus_tag="VN039_00020"
     CDS             complement(1271..1783)
                     /locus_tag="VN039_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44406.1"
                     /translation="MSDKYVASSPPALTPEEGDWVPNPIPHRPIAGNTADDDGIALDQ
                     MFESMELGPQSYEGAIPKEENPKHTTRLLVRTLPVDGTQPVQFLPRDPYRVALSIVAG
                     GGPTADAFVQTEQIMIGTSKGDTYGIGTISMPVLAGNGITLEGYTGALWINAQSLVAT
                     RNITVISHTR"
     gene            1884..2327
                     /locus_tag="VN039_00025"
     CDS             1884..2327
                     /locus_tag="VN039_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44407.1"
                     /translation="MSDSVVPTVPQVILSEDDLGAVQSFADAMQLMQDARVDVVSTDD
                     LGDGFVVLPTDGKASLVGVPFIILNMRFHTGDMGEFVILHVVTEDGRKLIVNDGSTGI
                     RDQCKGYARNNRTQGILCKRGLTRSDYTYEDDKGKSIPATTYYLG"
     gene            2957..>3690
                     /locus_tag="VN039_00030"
     CDS             2957..>3690
                     /locus_tag="VN039_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44408.1"
                     /translation="MERPFIAWDGEGADVDGRHRYTLLAHVRGGAIENPKGLGTIECL
                     EFMLNADVSDGINIVYGGNYDVNMILGDMPKDLLRTLYDTGKVYWREYRIEWRPGKQF
                     SVKHGERKFRLYDVLPFFQRPFVTACDEYLGTEWEYRDQIIAGKKRRGTFTAEESEYV
                     KEYNSAELRLLVRLASELRDRLNRVNLPEINEGIRLTRWDGPGAIATCLFQKYGVKKA
                     LGSVPEEVARAARHGYAGGRFEIIRQG"
BASE COUNT          971 a         1001 c          972 g          746 t
ORIGIN      
        1 gcacgtttgc ttccatgatc gaacctgccg tcatgggcat cccaaacgca gcaagagaca
       61 taccgacaag actgttcaac tgacccagta gttgagcggc agcacgagcc tgagactctc
      121 caaccttacc gacgccctta gagcctgacc ccttaggacg tccagcccca gcattaggcc
      181 gacgcttata cggagttccg tctgccttat acccataggg agcttcctcc gtagaacctg
      241 ggaaatcagg atcatacgat gacgctccct ccggcatggc aacgggaggc gcattatcct
      301 caggaatttc gacatcctta aaatcaagga tactagtcat cgtcctcgtc catatcctta
      361 gccagaccgt cgagaatgtc cgcgacctta gtcaaatgat cgaagcccac ttccgtagaa
      421 cactccgacg ccagattatg cgtaaccctt gcctgacgat gcaaccattc gcatacgtcc
      481 tgttccggcg tcatcagtga cccccgaaac cgcgatgcgt ccacggaaca gatacgggag
      541 tctcatcccg ctcctcttcc tcctcgtcat cgccgccggg aaggtccgga gtcagaagcc
      601 cggtcacctt gtcggaaagc tccgacacag aagcctcaat acggtcaaca cgctccgaca
      661 gaccagagtc ctcggcatgg tgatgagctt cctcaacagg agtctcagga gttacgtccg
      721 gaacaacgtc cggaacagcg tcggactcaa catgcatgtc gctcatgatt cacagtccca
      781 tcatcttgaa catagggtta ttcttaagac tttcaatcgt cggcgtaatc gcctcagaga
      841 tagcggtaat ctgcaacagg attccgtcaa gagtggtacg catagaagca acctcgctct
      901 caagagcggt aatacggtca ccgagcgaca tgtcactaag cggagcaatt ggagtgtcaa
      961 gagacatgag aaacaacctt actattaggg tggggattaa ctgacagaat cgcctcatag
     1021 agagcgacca atacgacgca cgaaacgagc gccgcgtaca gggggtaagg agacttgaca
     1081 aacgctcccg ccgtcaagcc aataaccagc gtaaccgccg atgatgcaac ctcagcagta
     1141 cgcacgtcct tacaggtgtt cacatcagca cgcgttgcac gccgaatagc ggcaacgtca
     1201 ggaagcatag ctgtgaatgc tccaacagaa acagtcacgg gaaccagtga cgcggagacg
     1261 actgccggac tcatcgcgtg tgggaaataa ccgtgatatt acgagtagca acaaggctct
     1321 gagcattgat ccaaagagcc ccggtataac cctcaagagt aataccatta ccagccagca
     1381 ccggcataga gatagtgcca atgccgtacg tatctccctt agatgttcca atcataatct
     1441 gttcggtctg gacaaaggca tcggcagtag gaccgcctcc ggcaacaata gacagagcaa
     1501 cgcggtaagg gtcacgcgga agaaactgga caggctgagt accgtctacc ggcagcgtac
     1561 ggactagcag acgagtcgta tgcttggggt tctcttcctt aggaatagcg ccctcatacg
     1621 actgcggccc aagctccata gactcaaaca tttggtccag agcgatgccg tcatcatcag
     1681 cggtatttcc cgcaatagga cgatgaggaa tagggttcgg aacccaatcc ccttcctcgg
     1741 gagtcagagc cgggggagag cttgcaacat acttgtcaga catggcttga ccttacgtgt
     1801 tgacaacgga tcgggatagg tctactatac caccagagcg ccggtcccgg acgctctcca
     1861 cccaagtaga taaggctgat tctatgtctg actctgttgt tcccacggtt ccgcaggtta
     1921 ttctctctga ggacgacctc ggcgctgtcc agtctttcgc ggacgctatg cagcttatgc
     1981 aggacgctcg cgtagacgtt gtttctaccg acgacctcgg agacggtttc gtcgtccttc
     2041 cgactgacgg taaggcatcg cttgttgggg tgcctttcat catccttaac atgcggttcc
     2101 acaccggcga catgggagag ttcgtcattc tccacgtcgt cactgaggac gggcgtaagc
     2161 tcatcgttaa cgatgggtct acgggcatcc gcgatcagtg caagggatac gcccgtaaca
     2221 accgtactca gggcatcctc tgcaagcgcg gtcttactcg ctccgattac acctatgagg
     2281 acgacaaggg taagagcatt cccgcgacga cctattacct cggctaatcc catcaggtaa
     2341 tgaaagtgct acaccccctg ccggatatct ggcagggggt gtagcattcg cctaggaagg
     2401 gcggtaggtg gctgacctat acgcccatga tagtcaggat tgggcgatat ggcaagcagg
     2461 aaacgcgatt atgcagcgga gtacgcgaga gccaaagcta aggcgcaagc ggcgggctat
     2521 aagtcacaac gagagtataa gcgcgcgcga gcaggtctaa cgggcagggt ttcccctgta
     2581 cccaagaccg taagggataa gattagagac gccgatagta aggcatggtc caacaagcat
     2641 agcaaatccc ctagctcgaa attcaatacg cgatgggggc gagaacgccg agacgcttac
     2701 tatcaagtat tcgttgagcc atacaataat cctcagcgtc cgaaagacgc taaagatacg
     2761 tccgatgttc gttggcgcaa agagttgcat aagatcctag ttccgagggt gtatagcgat
     2821 gcagaattta acgaacgcta cagcggctga tattcccacc ctagggagtc gcggtagaga
     2881 caagaaatac cgacagacag agcaaggaaa acagaatcgt ctagctcaga aacgtaggcg
     2941 cacgcaacgc gactacatgg aacgcccctt tatcgcatgg gatggggaag gcgcggacgt
     3001 agacggacgg caccgataca cgctcctagc acatgtacgc gggggagcaa tcgagaaccc
     3061 taaaggactc ggcacaatcg agtgtctaga gtttatgctc aacgctgatg ttagtgacgg
     3121 cattaacatc gtatacggcg gaaactacga tgtgaacatg atcctaggcg atatgcctaa
     3181 ggacctccta cgcacgctct acgataccgg taaagtttac tggcgcgaat accgcattga
     3241 atggcgtccg ggtaagcaat tctcagtaaa gcatggggag cggaaattta ggctctatga
     3301 cgtactccca ttctttcaac gtcctttcgt gacagcgtgt gacgaatacc taggcaccga
     3361 atgggaatac cgcgaccaaa tcattgcagg taagaaacgt cgcggaacct tcacagcaga
     3421 ggaatcggaa tacgtaaagg agtacaacag tgccgagtta cgtttgctcg tccgtcttgc
     3481 ttctgagctt agagacagac tcaatcgcgt taacctcccg gaaattaacg agggaattag
     3541 acttacaaga tgggacggac ccggagctat tgctacgtgt ctttttcaga aatacggcgt
     3601 caaaaaggcg ttgggatcgg tccctgagga agttgctaga gccgcacggc acggctacgc
     3661 tggcggacgt ttcgagatta tccgacaagg
//
LOCUS       DATMTI010000002         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_763579, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000002 DATMTI010000000
VERSION     DATMTI010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763579"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..864
                     /locus_tag="VN039_00035"
     CDS             148..864
                     /locus_tag="VN039_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWV44409.1"
                     /translation="MKVVLDIETVQAPRDEWVRLLGKPPANEGSYPAEGGFDLFSAGA
                     AEEQRRAEDESYAKSAFDGTFSRIVCIGLLEFSDQLEARSAVAWFGANERELLRQFWA
                     RLAQDRPALFITHNGLGFDLPFIKKRSIINQVKPSLDINLAKFRTEPVYDTMAIWSNW
                     DMRGWVKLDVLARALQVDTKSGSGEHVAEMWERRQGRELAQYCLQDTYVTYACYCRMN
                     FRQPLSREVVLLQPELYDVG"
     gene            complement(877..1869)
                     /locus_tag="VN039_00040"
     CDS             complement(877..1869)
                     /locus_tag="VN039_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44410.1"
                     /translation="MHAYFNVRGFRYVCAVMSLSLVVAGCSMFSGEAKLYKNAKGSVY
                     LKEIADWSFEAGHPATIDQMTMLKIVKGVVIDDVQKMSGNMPASGSKPMRVFSDEDAE
                     FLAPLLAQGLSQAKPEQIVGFKVSPSAGSGTEPTAGTLYMHKGSIYLTIAPSQTKKTA
                     GFTPSSVARLEKTPSFVALGSGAMTMVIDYQALAKGPMPSAPYAASKPMPVSTSQSAP
                     VTPTAMPAPSASPVKIAPPQEDSIGDPRTDSGTPQLSTDELLNRKLDELREAREANKL
                     KDSEIAMLKKETTWMKKELRQRTEEVKTLKTSKTAVRSAPSAPRKKSAEAYPTR"
     gene            complement(1909..>2090)
                     /locus_tag="VN039_00045"
     CDS             complement(1909..>2090)
                     /locus_tag="VN039_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44411.1"
                     /translation="CPSVRQRGISLLVIMKSADSESKPNPNPSDSGITLETIISVGVV
                     GWLAIGMVLMALGIW"
BASE COUNT          444 a          529 c          594 g          523 t
ORIGIN      
        1 atgtgccatt gaccaagggg agccaatacc tattggctgt gaagaaatcc gatgggtata
       61 ccccgaagat ctcacatgct ttacgttccc gcctgcagac tacgcggtca tcgaagcctt
      121 acggcgcgat gcgttcggag tttcgcgatg aaggtggtgc tcgacattga aaccgtccag
      181 gccccacgcg atgaatgggt tcgtctattg gggaagccgc cggcaaatga agggtcttat
      241 ccggcagaag gaggcttcga tctcttttcg gcgggagctg ctgaagagca acgtcgcgcg
      301 gaggatgagt cgtatgccaa gtcggcgttc gatggaacgt tcagccgcat cgtctgtatc
      361 ggcctgctgg aattttccga tcagctggaa gcacgcagtg ccgtcgcatg gttcggagcg
      421 aacgagcgtg agctgctccg ccaattttgg gccagactag cccaggaccg acctgccttg
      481 ttcatcacgc ataacgggct tggtttcgat cttcccttca tcaagaagcg gtcgatcatc
      541 aatcaagtga aacccagcct cgacatcaat ctcgcaaaat tccgaaccga accggtctat
      601 gacacgatgg ccatctggag taattgggat atgaggggtt gggtcaagct ggatgtattg
      661 gcccgcgcat tacaggtcga tacgaagtcg ggcagtgggg aacacgttgc tgaaatgtgg
      721 gaaaggaggc aaggtcgtga gcttgcacag tactgtctgc aagataccta tgtaacttat
      781 gcctgctatt gccgcatgaa ctttcgtcag cctctgtcca gggaggttgt tctgttgcag
      841 cctgagttgt acgatgtcgg ttgattgatc ggtcgatcaa cgggtcggat acgcttccgc
      901 cgattttttt ctcggggctg acggggctga acggacggct gtcttactgg tcttcaaggt
      961 tttcacctct tccgtccgtt gacggagctc tttcttcatc cacgttgttt ctttcttcag
     1021 catcgcaatc tcagagtcct taagcttgtt ggcctcgcgc gcttcccgga gctcatcgag
     1081 tttcctgttg agcagttcat cagtgctcaa ttgaggagtt cctgaatcag tccgagggtc
     1141 cccgatagaa tcttcctgtg gcggtgcaat tttcacaggt gaagcgctcg gtgccggcat
     1201 cgcagtcggt gtgactggag cggactgaga cgtggaaacc ggcatgggct ttgaggctgc
     1261 atagggtgcg ctcggcatcg gtcctttggc aagggcttgg tagtcgatca ccatcgtcat
     1321 cgctccgctt cccagcgcaa cgaaagaagg agttttttca agacgagcaa cagaactggg
     1381 agtgaatccc gctgtcttct tggtttgaga aggagcgatg gtcagataga tcgatccttt
     1441 atgcatatag agggtgcccg ctgtgggttc agtgcctgac cctgccgatg gagacacctt
     1501 gaaaccgaca atctgctccg gtttcgcttg cgataagcct tgggctagca ggggagcaag
     1561 aaactcggcg tcttcatcgc tgaatactct catcggcttg ctgccactgg cgggcatgtt
     1621 gccggacatt ttttgaacgt cgtcgatcac aacgcctttg actattttca gcatcgtcat
     1681 ctggtcgatg gtggctggat ggccggcttc gaatgaccaa tcggcgattt ccttgagata
     1741 gaccgatcct ttcgcatttt tataaagctt tgcctctccg gaaaacatcg agcatccggc
     1801 cactacgagg ctgagggaca tcaccgcaca gacatatcgg aatccgcgga cattgaaata
     1861 tgcatgcata tgggatttcc tcacatggtc ttgtgatcgg atgtcgacct accagatacc
     1921 tagtgccatg agtaccatgc cgatcgccaa ccatccgact actccgaccg aaatgattgt
     1981 ttcgagtgtg atgccggagt cggacgggtt tggattgggc tttgactcgg agtcggcgga
     2041 cttcataata acgagaagcg aaattcctcg ttgccgaaca gatggacacc
//
LOCUS       DATMTI010000003         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_763580, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000003 DATMTI010000000
VERSION     DATMTI010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763580"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1356)
                     /locus_tag="VN039_00050"
     CDS             complement(151..1356)
                     /locus_tag="VN039_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV44412.1"
                     /translation="MTIAIHQVFLIDGTGDLHDNATVLVRGTKILAAGPSNEVRIPKG
                     AVRIDGRGLSMIPGLIDCHVHLCLGGEPDVVGTLESEQPSYTLLKSATHAKATVEAGF
                     TTVRDVGSRDHSIFTLKQAIDSGLLPGPRIVGAGLVICMIGGHARFIGQEVEGTEQVR
                     RVVAEQVAAGAGVIKIIASGGVLTPGTSPDEAQMTMEELAAAVDAAQQAGKRVAAHAH
                     GAAGMKNAIHAGVRSIEHATLLDEESGALMKRYGVYMVPTLSALATTAACRPGCGIPE
                     GALAKAKSMTKRHQASFKAAHQSGIAIAMGTDAGTPFNYHGENAQELERMVAFGMTPM
                     EAIVASTATAAQLIGIQDSVGTLTKGREADLVILKGNPLRRIGILRNRDKIVGVMKAG
                     KFVAGVLAK"
     gene            complement(1353..>2481)
                     /locus_tag="VN039_00055"
     CDS             complement(1353..>2481)
                     /locus_tag="VN039_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWV44413.1"
                     /translation="HPRVLQALATPLVGHLDPTFLNVMNDIQALLRRVFSTTNQFTIA
                     VSGTGSAGMEAAVVNVVEPGDAVIVGINGVFGTRLAAIVERCGGKVIRVEVPWGQVIE
                     PDTIAAALRQSSPVKAVALVHAETSTGAWQPLEPISALCRQYNALLIVDAVTSLGGIP
                     VEVDRWSIDVCYSATQKCLSCPPGLAPLTLSERALSVVKNRRSPCQSWYFDLSLIADY
                     WTERKRAYHHTAPISMLYALREALRLVEEEGLPARFARHQLNSRALLAGLMALGLDQL
                     PPLDRQLPTLICVTIPAHIDEAAVRSQLLETFGIEIGGGLGPLKGKVWRIGLMGESST
                     EANVLTLLNAMEEIGIRSGWVSTPGAALQAAAHIYSGGDKV"
BASE COUNT          505 a          757 c          697 g          522 t
ORIGIN      
        1 cctccgcgag atcgattgca cgcctgaacg atttcgtcat gaactggaaa acctctgatc
       61 gttgctcggc caaccagctg tcggcttcgg ttctcctagc tttgaatggc aatgcgcggt
      121 taccgccatg acaaatgacg accgatgacc ttacttcgcg agcactcccg caacgaactt
      181 gcccgccttc atcacgccta caatcttgtc ccggtttcgt agtatcccga tccgtctgag
      241 tggatttcct ttcaggatca cgagatcggc ttcccttcct ttggtcagtg tgccgactga
      301 atcctgaatc ccgatgagct gggctgccgt ggcggtcgag gcgacaatgg cctccattgg
      361 cgtcatgcca aaagcaacca tgcgttcgag ttcttgcgca ttttctccat gatagttgaa
      421 gggggttcca gcatcggtac ccatggcgat ggcgatgccg ctctgatggg cggctttgaa
      481 gctggcctgg tgtcgcttgg tcatggattt ggctttggcc aatgcacctt ccgggatgcc
      541 gcagccaggc cggcatgccg ccgttgtagc caaagccgaa agggtaggga ccatgtagac
      601 cccatagcgc ttcatgagtg ctcccgactc ctcgtccaat aaagtcgcat gttcgatcga
      661 gcgtaccccc gcatggatcg cgttcttcat tccggcggca ccgtgggcat gggcagcaac
      721 tcttttcccg gcttgttgtg ccgcatcgac cgctgcagcc aactcctcca ttgtcatttg
      781 agcctcatcc ggcgaggtgc cgggcgtcag caccccccct gaggcaatga tcttgatgac
      841 ccccgcaccg gctgcgacct gttcggccac aacccgtctg acctgctcgg tgccctccac
      901 ttcctgacca atgaaccgtg catggccgcc gatcatgcag atgacaagac ctgcaccgac
      961 aatacgtggc ccaggcagaa gacccgaatc gatcgcctgt ttcagtgtga agatggaatg
     1021 gtcccgagat ccaacgtcgc gcaccgtggt gaatccggcc tcgaccgttg ccttcgcatg
     1081 tgtggccgac ttcaacaacg tataggaagg ctgctctgat tcgagcgtgc cgacgacatc
     1141 cggctctccg cccaagcaaa gatggacatg acagtcgatg agtcctggga tcatcgagag
     1201 gccgcggccg tcgatacgga ctgctccttt ggggattctg acctcgttgc ttgggccggc
     1261 tgccaggatc ttggtgccac gcacgaggac cgtcgcgtta tcgtgaaggt cacccgtccc
     1321 atcgatgaga aacacctgat gaatagcaat cgtcacacct tatcgcctcc actataaata
     1381 tgcgctgcgg cttgcagggc agcgccggga gttgaaaccc acccgctccg aataccgatc
     1441 tcctccatgg cgttcagcag agtcagaacg ttggcctccg tcgacgactc tcccattagt
     1501 ccgattctcc agacctttcc cttgagaggc ccaaggcctc cgccgatttc gataccgaac
     1561 gtttccagca actgggatcg gacggctgcc tcatcgatgt gtgccggaat ggtgacgcag
     1621 atcagcgtcg gaagttgtcg atcaagggga ggtaactggt cgagaccgag cgccatcagt
     1681 cctgcgagca gcgcacggct attgagttga tggcgagcaa accgggccgg aagtccttct
     1741 tcctcgacca gacgcagcgc ctctcgcagg gcatagagca tcgagatcgg tgccgtatgg
     1801 tgataggcac gcttacgttc cgtccagtaa tctgcgatga gggacaggtc aaaataccag
     1861 ctttgacaag gagagcgacg attcttgacg acggaaaggg cgcgctcgct taacgtcagg
     1921 ggagccaagc cgggcgggca gctgagacat ttttgcgtgg cgctgtagca cacatcgatg
     1981 ctccaccgat cgacctccac agggatgccg ccaagcgagg tgaccgcgtc gacaatcagc
     2041 agcgcattgt actgacgaca aagggcactg atcggttcga gaggttgcca cgcccccgtc
     2101 gaagtttcgg catgcaccag cgcaacggct ttgaccggac tcgactgccg gagcgccgcg
     2161 gcaatcgtat ccggttcgat cacctgcccc cacggaactt ccactcgaat gactttccct
     2221 ccgcagcgct caacgattgc cgcgagtctg gttccgaaca cgccgttgat gcctacaatg
     2281 acggcatcgc ccggttcaac gacgttcacg accgcagcct ccatgccggc tgatccggtc
     2341 ccggaaacgg caatcgtaaa ttgattggtc gtcgagaata cgcggcgcaa gagcgcctga
     2401 atgtcgttca ttacgttcaa gaatgtcgga tccagatgtc cgacaagcgg agttgcgagg
     2461 gcttggagta ctcgaggatg c
//
LOCUS       DATMTI010000004         1916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_636341, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000004 DATMTI010000000
VERSION     DATMTI010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636341"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1471
                     /locus_tag="VN039_00060"
     CDS             128..1471
                     /locus_tag="VN039_00060"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV44414.1"
                     /translation="MRLRPIIVFVLAWCIPMAESAFGGMASYIQQSQTLYDKKEYQKA
                     LAELDKLDSQTSAAPDARRLRIRTLFKLCNPKDALTNYDELVQALKHEDQSILRELSL
                     GFVLVLTKDMREQMRGVAYTALKEWHSSESIPYLEDGLSDGSGLVRALAAEGLAKLDE
                     GRRSARFRKALEDQAALVKEAVLKGLAKSDDPSVIPLVEPAMKDPEVRVRVAAAAVLC
                     NLKQPKGCDLLLQYAKASNPDERTSAIRALADRQSSEVLSILIESSEHKQPSVRGAAA
                     TGFAHVPAAEAITALTRLLRDPLPPVRIAAAVSLGQLHGFDARTPLHGALDDRDLPVR
                     AFVIGALLEQGDRYEIVAGPVQTLSNTKEPAVRAAVARALGHAVESNRESARSALMLL
                     VQDTVPRVRIAAVKSMVKIDGTGAIQLLKQSLHDEDDAVRATAGGALLKVVLVNE"
     gene            1509..>1916
                     /locus_tag="VN039_00065"
     CDS             1509..>1916
                     /locus_tag="VN039_00065"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWV44415.1"
                     /translation="MVVRILAALALSCFWFDASVLSANEQPAHVLTEPLTGKDGAPMI
                     VVPAGSFPMGVPAGARDGGRDEYPRHDVFVDTFAIDKFEVTNGRYLEFVKATGHRVPQ
                     NPRNPTRNLWQGDSITDVLTDRPVINVDWFDAEA"
BASE COUNT          419 a          513 c          577 g          407 t
ORIGIN      
        1 caatattctc ccctttgagc cggaattctc cggcatgctc gtcaaacgca tacacggcaa
       61 gaaataatca ccaaggcatg aatataaccg gcggcaccga gaagggtaga ggatggaccc
      121 cctgaacatg cgactacgtc caatcatcgt gtttgtcctg gcctggtgta ttcccatggc
      181 tgagtccgcg tttggcggaa tggcatcata cattcagcag agtcagacac tctatgacaa
      241 gaaggagtat cagaaggcac ttgcagagtt ggataaactc gattctcaga cgtcggccgc
      301 accggacgct cgtcgactca gaattcgtac actgtttaaa ctgtgcaacc ccaaggatgc
      361 cctcaccaac tatgatgagc tcgtacaggc attgaagcat gaggatcaat caatccttcg
      421 tgagctctct cttggatttg tcctcgtctt gacgaaggac atgcgcgagc aaatgcgtgg
      481 tgtggcttat accgccctca aggaatggca tagctccgag tcgattccat atttggaaga
      541 tgggctcagc gacgggtccg gtctcgtccg agccttggcg gctgaagggc ttgcgaaact
      601 tgacgagggc cgccgctcgg ctcgattccg gaaggctctg gaagatcagg cggcgttggt
      661 gaaggaggcg gtactcaagg gacttgcaaa atctgatgat ccttcggtca tcccgctcgt
      721 cgagccggcg atgaaggatc ctgaggtacg ggtacgagtt gcagccgcgg cggtgctctg
      781 taatctgaaa caacccaagg gctgcgatct actcttgcag tatgcgaaag cgtcgaatcc
      841 ggatgagcgg acctcggcaa ttcgtgcctt agctgatcgg cagtcgtcag aagttttgtc
      901 gattctcatc gaatcgagcg agcacaaaca gccgtcggtg cgcggggctg ccgccacagg
      961 gttcgcccat gtacccgctg ccgaagccat aactgcgctg acgcggctgc tgagagaccc
     1021 gcttcctccc gtgcggatcg ccgcagccgt cagcctgggg cagctgcacg gattcgatgc
     1081 ccgtacgccg ttgcatgggg cgcttgatga tcgtgatctc cccgtcagag cgttcgtcat
     1141 cggtgcgcta ctggaacagg gcgaccggta tgagatcgtg gccggcccgg tccagactct
     1201 cagcaacacg aaagagccgg ccgttcgtgc tgccgtggca cgagcgttgg ggcatgccgt
     1261 cgaatcgaat cgagagtcgg cacgatcggc cttgatgctg ctggttcagg atacggtgcc
     1321 tcgagtgagg attgcggcgg tcaagtcgat ggtgaaaatt gacggcaccg gtgcgataca
     1381 actcctcaaa cagagcctgc acgatgagga tgatgccgtg cgggcaacgg ccggaggagc
     1441 gctgttgaaa gttgttctag tgaacgaata gggtccagcg ggctgcggga gaacaaggag
     1501 ttgtgcccat ggtcgttcga attcttgctg cgttggcact gtcatgcttt tggttcgatg
     1561 cgtccgttct gtctgcaaat gagcaaccgg ctcatgttct gaccgagccg ttgacgggca
     1621 aagacggcgc accaatgatc gtggtccccg ccggctcgtt cccgatgggt gtaccggccg
     1681 gagcccgtga cgggggacgt gatgaatatc cgcgccacga cgtgttcgtc gacacttttg
     1741 cgatcgacaa atttgaagta acaaacggcc gttatctgga attcgtaaag gccacagggc
     1801 atcgcgttcc acaaaaccca agaaatccga caagaaatct ttggcaaggg gacagcatca
     1861 ccgatgtgtt gaccgatcga cccgtcatta acgttgattg gtttgatgcc gaggcc
//
LOCUS       DATMTI010000005         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_594137, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000005 DATMTI010000000
VERSION     DATMTI010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594137"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(434..1390)
                     /locus_tag="VN039_00070"
     CDS             complement(434..1390)
                     /locus_tag="VN039_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019437692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWV44416.1"
                     /translation="MPQQTFAEVPFEQYRKSTRRERFLDEMNRVVPWADLVAAIEPIY
                     PKAEGPGRPPVGVERMLRLHCLQQWFNLSDPAVEEALYDSRAMRQFVGIDLGREPVPD
                     ETTICKFRHLLEAHQLGEQLFARIGQHLAKHGLQVNHGTIVDATIISAPSSTKNRSKE
                     RDPEMHQTKKGTQWYFGMKAHIGVDSQTKLIHSVAATAANVHDSQVLPKLLHGQETRV
                     WGDAAYSGQRDVIRHHAPRAKSFVQTKAHRYRPLREEERARNRTKSKVRAKVEHAFLV
                     IKRIFGWAKVRYRGLAKNTHWLHISCGLANLYVARRRLLPGT"
     gene            complement(1614..>2078)
                     /locus_tag="VN039_00075"
     CDS             complement(1614..>2078)
                     /locus_tag="VN039_00075"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HWV44417.1"
                     /translation="IMGNSTHYDIFVTHAWRFHEDWTKFTELMDKMPGLLWRNFSLPW
                     HDPAISPNTEAGGRFIRSSLESQIIPVHVVVLLAGVYEIRSARLWVDMEVEMAKRHNK
                     PIIALPAINKGSIPDELVALCDATSGWDAARLIATIDEVRSLPRYALQSSGR"
BASE COUNT          446 a          623 c          534 g          475 t
ORIGIN      
        1 cttgaaaagt tcatgccctc cgactccatc tgtccgtgac gctctcgatg gcgcaaggcc
       61 atcggaatgg cacctaatgt gacgagcgaa tcccaaggga tgatgtgaac gggggtgctg
      121 cgttatagat cagtccaacc ggaacatggt gtagagacac tgttcattct gacacgcgtc
      181 tttccaaaga aatctcttca aggctcgcat accgaccttt attcacccac gcagttcgtg
      241 tgccgaagat tgtgagagct gtggagcgac tcacgtctta ggaaaggtct gattaattca
      301 cgtttccact cggaaatcta ttcctgaact ggtgaccaag gcaaattcga tggacatccg
      361 ctggtccggg agtcgttcgt gaggtgtcct caccgtccga tggctcgttg tcagccttcc
      421 aggcatacgt tctctacgtc ccgggcaaga gccgccgtcg cgccacgtac aggttcgcca
      481 acccgcagct gatatggagc cagtgggtgt tcttcgcgag ccctcggtac cggaccttgg
      541 cccacccgaa gatcctcttg atcaccaaga acgcatgctc tactttagcc cggactttcg
      601 atttcgtccg gttccgtgcc cgctcctcct cacgcagcgg ccgatagcga tgcgctttcg
      661 tctggacgaa gctcttggcc cgtggcgcat ggtgccggat cacgtcacgc tgcccgctgt
      721 aggcggcatc cccccacacc cgcgtctcct gtccatgcag caacttcggt aacacctggc
      781 tgtcatgcac attcgcggca gtcgcggcta ccgaatgaat cagtttcgtc tggctatcca
      841 ctccaatatg ggccttcatg ccgaaatacc actgggtgcc cttcttggtc tgatgcatct
      901 ccggatcccg ctccttcgaa cgattcttcg tcgaactggg ggcactgatg atggtcgcat
      961 ccacgatggt cccatggttc acctgcaggc cgtgcttcgc cagatgctgc ccaatccgtg
     1021 caaaaagctg ttcgcccagt tggtgggctt ccaagagatg ccgaaacttg cagatcgtgg
     1081 tctcatcggg cacgggctca cgacccaggt caatgcccac gaactgccgc atggcgcgtg
     1141 agtcatacag cgcttcttcg acggcggggt ccgacaggtt gaaccactgt tgcagacaat
     1201 ggaggcgcaa catgcgttcg acgccaaccg gcggacgccc cggcccctca gccttgggat
     1261 agatcggctc gatcgccgcc accaggtctg cccacggtac gacacgattc atttcgtcga
     1321 gaaaccgctc gcgacgggtg gatttgcgat actgctcgaa ggggacttca gcaaaagtct
     1381 gttggggcat gggtgcgcct cccgttcagt gctgcactac ccgtagcaca tcacgcaaag
     1441 agaataaatc agaccttcct taagatacac aagcaggtgt gcggtatcaa tcgactttct
     1501 gactcacagc cacataatca tacctgcatc aagtcgcaag gatatctccc tctggcccac
     1561 actctttttt caataagcag ttcagcgcca gtccaggaaa catcaaggtc gaatcatcgc
     1621 cctgaagact gcagtgcata tcttgggaga gaccgcactt cgtcaatggt ggcgatcaat
     1681 cgtgccgcgt cccatccgct ggtcgcgtcg cacaacgcaa cgagttcgtc cggtatcgag
     1741 cccttgttga tcgcaggcaa agcgataatg ggtttgttgt gcctcttcgc catttcgact
     1801 tccatgtcga cccacaatcg ggcgctccta atttcgtata cccccgcaag cagaacaacc
     1861 acatgcaccg gaatgatctg gctttccaag gaactgcgga tgaatcgacc tccggcttcc
     1921 gtattcggac ttattgcagg atcatgccag ggcaggctga agttgcgcca cagaagtccc
     1981 ggcatcttgt ccataagttc tgtaaatttt gtccaatcct catgaaaacg ccaggcatga
     2041 gtaacaaaga tgtcgtaatg agttgagttt cccataat
//
LOCUS       DATMTI010000006         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_169966, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000006 DATMTI010000000
VERSION     DATMTI010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169966"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VN039_00080"
     CDS             <1..1027
                     /locus_tag="VN039_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWV44418.1"
                     /translation="RLTMFQSRASAFINPKSGDKMKLNRRTFTAALSASSLAGLLPVT
                     AFAEKQIVLGFAQVGAESEWRTANTLSIKSAAKDAGIELKFSDAQQKQENQIKAIRSY
                     IAQKVDVIGFSPVVVTGWDPVLREAKAAKIPVVLTDRAIDSKDTSLYVTFLGSDFIEE
                     GRKAGRWLLQDMKDTKGNINIVELEGTVGSAPAIDRKKGFDEIIKTDPRFQVIRSQSG
                     DFTRAKGKEVMEAFLKADGKKINVLFSHNDDMAIGAIQAIEEAGLKPGKDITIVSIDG
                     VKGAFEAMMAGKLNCTVECNPLLGPQLMGIVKDIVAGKAVPKRIVTKEGIFPMDVAKA
                     EFPNRKY"
     gene            1165..2238
                     /gene="chvE"
                     /locus_tag="VN039_00085"
     CDS             1165..2238
                     /gene="chvE"
                     /locus_tag="VN039_00085"
                     /inference="COORDINATES: protein motif:HMM:NF040907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HWV44419.1"
                     /translation="MKRTFLKTLLAGTAATVLMGLAPLAHAQDKGLIGISMPTKSSSR
                     WIADGNNMAKDFQAKGYKTDLEYAEDDIPTQLAQIEDMLTKGAKVLVIAAIDGTTLSH
                     VLQEAADKGVKVIAYDRLIKGSKNVDYYATFDNFQVGVLQANYIVKALKLQEGKGPFN
                     IELFGGSPDDNNAFFFYNGAMSVLKPYIDSGKLVVRSKQMGMDKVSTLRWDGAVAQAR
                     MDNLLSAYYTKDRVDAVLSPYDGLSIGIISALKGVGYGTAQLPMPVVTGQDAELPSIK
                     SILKKEQTSTVFKDTRELAKVTVAMAVAMLAGEQPVVNDTKTYNNGVKVVPAYLLKPV
                     IVDASNWKHVLVDSGYYKESQIQ"
     gene            2250..>2450
                     /locus_tag="VN039_00090"
     CDS             2250..>2450
                     /locus_tag="VN039_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV44420.1"
                     /translation="MNNVILEMRGITKTFPGVNALDNVNFAVKSGEIHAVVGENGAGK
                     STLMKVLSGVYPCDSYTGEILFE"
BASE COUNT          576 a          689 c          711 g          474 t
ORIGIN      
        1 tcgcctgaca atgttccagt cccgtgcaag tgctttcatc aaccccaaat caggagacaa
       61 aatgaaattg aatcgtcgta cctttactgc cgccttgtcc gcctcgtccc tcgcgggact
      121 gttaccggtc acagcgtttg cggaaaaaca aatcgtgctg ggctttgccc aggtcggcgc
      181 cgaaagtgag tggcgcaccg ccaataccct atcgatcaag tccgctgcca aagacgccgg
      241 catcgaactg aaattctccg atgcccagca aaagcaggag aaccagatca aggcgattcg
      301 ctcgtatatt gcccaaaagg tcgacgtaat cggcttttct ccggtggtcg tgaccggttg
      361 ggatccagtc ctgcgtgagg ccaaggccgc aaaaatcccg gtggtactca ccgatcgcgc
      421 catcgacagc aaggacacct cgctgtatgt gacgttcctg gggtcagact tcatcgagga
      481 aggacgcaaa gccggccgct ggctgctgca agacatgaag gatacaaagg gaaacatcaa
      541 tatcgtggaa ctggagggca ccgtcggatc ggcaccagcg atcgaccgca agaaggggtt
      601 tgacgaaatc attaaaaccg acccgagatt ccaggtcatc cgctcgcagt caggtgactt
      661 tacgcgggcc aagggcaagg aggtgatgga ggctttcctg aaggccgacg gcaaaaaaat
      721 caacgtgctc ttctcgcaca acgatgacat ggcgattggt gcgatccagg cgatcgaaga
      781 ggccggcctc aagcccggca aggacatcac gatcgtctcg attgatggcg tcaagggcgc
      841 ttttgaagcc atgatggccg gcaagctgaa ctgcacggtt gaatgcaacc cgttgctcgg
      901 cccccaattg atgggcatcg tgaaggacat cgtggcgggc aaggctgtgc ccaagcgcat
      961 cgtcaccaag gaaggcatct ttccgatgga tgtcgccaaa gccgaatttc cgaaccggaa
     1021 atactgatct cgggttcgcc ctcttggggg cgaactcacc gtttgcccca ggcaacgtca
     1081 gtggaccttc tggatcgggt ccgtggtgct tcggaagcac ctcgactctc gacatcattc
     1141 gtactcgtta cacggaaaca tgatatgaaa cgtacctttc tcaaaaccct gcttgctggt
     1201 accgctgcca ctgtgctgat ggggctcgca cccctggctc acgcacagga caagggactc
     1261 atcggcatct cgatgccgac caagtcgtcg tcgcgctgga tcgcagacgg caacaacatg
     1321 gccaaggatt tccaggcgaa gggctacaag accgatctgg agtatgccga agacgacatc
     1381 cccacccagt tggcccagat cgaggatatg ctgaccaagg gtgccaaagt gctggtgatc
     1441 gccgcgatcg acggcaccac cctgtcccac gtgttgcagg aggcggccga taaaggcgtc
     1501 aaggtcatcg cttacgaccg actcatcaag ggctcgaaga atgtcgacta ctacgccacc
     1561 ttcgacaact tccaggtcgg cgtgctgcag gcgaattaca tcgtcaaggc tctcaagctc
     1621 caggagggca aaggtccgtt caatatcgaa ttgtttggcg gctcccccga cgacaacaac
     1681 gcgttcttct tctacaacgg agcgatgtcg gtgctcaagc cctatatcga cagtggcaag
     1741 ctggtcgtgc gcagcaagca aatgggtatg gacaaggtct ccacgctgcg ctgggatggc
     1801 gccgttgcgc aggcgcgtat ggataacctg ctgtctgcct attacaccaa ggatcgcgtg
     1861 gatgccgtgc tgtcgcccta cgatggactg tcgatcggca tcatctcggc gctcaaggga
     1921 gtgggctatg ggactgcgca actgccgatg ccggtggtga cgggccagga tgccgagctg
     1981 ccctcaatca aatcgatctt gaagaaggag cagacctcga ccgtgttcaa ggacacgcgg
     2041 gagcttgcca aggtgacagt tgccatggcg gtcgccatgc tcgcaggcga gcagcccgtg
     2101 gtgaacgaca ccaagaccta caacaacggc gtcaaggttg tgcccgccta cctgctcaag
     2161 ccggtgatcg tcgatgcatc gaactggaag cacgtgctgg tcgacagcgg ctactacaag
     2221 gagagccaga tccaatagga gccggtcgcg tgaacaacgt cattctggag atgcgcggca
     2281 tcaccaagac ttttcccggg gtgaatgccc tggacaacgt caatttcgcg gtgaagtccg
     2341 gtgaaatcca tgcagtggtg ggtgaaaacg gggcgggaaa atccacgctg atgaaagtcc
     2401 tcagtggcgt ctatccgtgt gacagttaca caggtgagat tctatttgag
//
LOCUS       DATMTI010000007         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_254861, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000007 DATMTI010000000
VERSION     DATMTI010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254861"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..172)
                     /locus_tag="VN039_00095"
     CDS             complement(11..172)
                     /locus_tag="VN039_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44421.1"
                     /translation="MDSTDTSIEAVRARITKWKENARTNRDRYDITSELWTYYDSQII
                     AYSDVLSIL"
     gene            complement(310..483)
                     /locus_tag="VN039_00100"
     CDS             complement(310..483)
                     /locus_tag="VN039_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44422.1"
                     /translation="MAYTVILRRGAGHAFPYLDTNEYDLAMSIRERLSVECETLGNGI
                     KAEVWSRESGRIA"
     gene            complement(599..823)
                     /locus_tag="VN039_00105"
     CDS             complement(599..823)
                     /locus_tag="VN039_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44423.1"
                     /translation="MDNTERAITQLYEWANEPGNDMHDEVDLLVTDYRRAVRKLSEAV
                     QLAEFWEHCYNAERNLAEIRAKLIEQLENS"
     gene            complement(898..1548)
                     /locus_tag="VN039_00110"
     CDS             complement(898..1548)
                     /locus_tag="VN039_00110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWV44424.1"
                     /translation="MLYPNGTTTKPKVSSPFGPRKGSSQNGFHYGTDYIGFPDVHSIA
                     AGTVTLARYLNDQAGNTVAVDIAHGNGTTTTIVYMHLATINVKVGQSVSEGQILGAVG
                     QTGNATGPCCHLEVRIWIAGKCHWQDSEPWLAARVGVKPVRLAMATCNIRKTPSTALA
                     PVGKFAAGTRVQPVGYVHGQTVENNACWYKLDSGYYVWTGGFVNGAGGVAGVPFQG"
     gene            complement(1548..1682)
                     /locus_tag="VN039_00115"
     CDS             complement(1548..1682)
                     /locus_tag="VN039_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44425.1"
                     /translation="MPNADGTPTDPEIRLSILGMLSYQSIPDPTTAETLFNWVKNGAA
                     "
     gene            complement(1682..1987)
                     /locus_tag="VN039_00120"
     CDS             complement(1682..1987)
                     /locus_tag="VN039_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44426.1"
                     /translation="MSPRRPVLPNVWYIDPASIDIGDTIRASSVIGDLKIEKVGVVHR
                     ILFDGPIRVIVTRDGQEITRWQHGVKHDRIALLDKAPLPQPALFEWGTTTNLNERLA"
     gene            complement(1974..2153)
                     /locus_tag="VN039_00125"
     CDS             complement(1974..2153)
                     /locus_tag="VN039_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44427.1"
                     /translation="MDTARYRVLVVDPHRPGAPHQADRAQLLQQTREGDYIIIATGTY
                     ANMKGIAGVMNHVPA"
     gene            complement(2261..>2558)
                     /locus_tag="VN039_00130"
     CDS             complement(2261..>2558)
                     /locus_tag="VN039_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44428.1"
                     /translation="PPVLASSLTPRTDYQESLPKTVRITSTKFRRILSYSMYALAGVS
                     GAASLFFIYFPEVANGSTIPDRVIAFINALISTASGIFGVSVSAPNIGRAESNG"
BASE COUNT          476 a          683 c          807 g          592 t
ORIGIN      
        1 gcacgggagg tcagaggatc gagagtacgt cgctatacgc gatgatctgt gagtcgtagt
       61 acgtccacag ttcagatgtg atgtcgtacc ggtcgcggtt tgtgcgggcg ttttctttcc
      121 actttgtaat gcgcgcgcgg acggcttcga tgctggtgtc tgtgctgtcc atatctctat
      181 tatgacatgg gccgtttgtg aatgtcaagt cctagccgtg gaaattgtcc caccacgcga
      241 tgccaaccca tatgaggatg aggccgatga atgcgcctac gaggagtgcc cacaccattt
      301 cgatgttact catgcgatcc tcccgctttc cctagaccac acttccgctt tgattccgtt
      361 gccgagggtc tcgcattcga cgctgagacg ttcacgaatg gacatggcga ggtcatattc
      421 gttggtgtcc agatacggga acgcgtgacc cgcaccacgt ctgagaatga cggtgtatgc
      481 catggttatc ctccgaggtt gtagagggct gagtagatgg cgccgacggc gaggccgagg
      541 gcgatggcgg cgggaagcat gaggaaggcg gcgagtttgc ttgtgccgtt gtctttgttc
      601 atgagttctc caactgttcg atgagctttg cccggatttc ggcgaggttt cgttcggcgt
      661 tgtagcaatg ttcccagaat tcggcgagtt gcacggcttc ggagagtttg cgtacggcgc
      721 gccggtagtc ggtgacaagc aaatctactt cgtcgtgcat gtcattgccg ggttcgttcg
      781 cccattcgta gagttgcgtg atggcccgtt cggtgttgtc catgcttcga ttctacacac
      841 gtcaaacgcc gcatgtcaac tatcaacatg cggcgtttga atgtaccgtt tggtacgtta
      901 gccctggaac gggaccccgg ccactccccc cgcgccgttg acaaatccgc cggtccacac
      961 gtagtacccg ctgtcgagtt tgtaccaaca cgcgttgttc tcgacggtct gcccgtgcac
     1021 gtacccgaca ggctggacac gtgttccggc ggcgaacttg ccgacggggg cgagtgcggt
     1081 ggagggtgtc ttgcgaatgt tacacgtcgc catggcgaga cgcacaggct tgacaccgac
     1141 acgcgcggcg agccatggtt cggagtcctg ccagtgacac ttgccagcga tccagatgcg
     1201 gacttccagg tgacagcatg gaccggtggc gttgcctgtc tgcccgaccg caccgaggat
     1261 ctgcccttcg ctcacggact gtcccacctt gacattgata gtggcaaggt gcatgtagac
     1321 gatggtggtt gtggtgccgt tgccgtgtgc gatgtccacg gcgaccgtgt taccggcctg
     1381 gtcgttcagg taccgggcga gggtgacggt tcccgcggcg atgctgtgca cgtcggggaa
     1441 accaatgtag tcggtgccgt agtggaaacc gttctgactg gaccccttgc gtgggccgaa
     1501 cggggatgag accttgggtt tcgtggtggt tccgttgggg tacaacatta tgcggccccg
     1561 ttcttaaccc aattgaacag ggtttctgcg gtggtgggat cggggatgct ctgataggag
     1621 agcattccga gaatgctcaa acggatttcg gggtccgtgg gagtgccgtc ggcgttaggc
     1681 attatgcgag cctttcattc aggttggttg tcgtgcccca ttcaaaaagg gcaggttgcg
     1741 gaaggggagc cttgtccagc agagcgattc tatcgtgttt gactccgtgt tgccagcggg
     1801 tgatttcctg cccgtcacgg gtcacaatga cccggatggg accatcgaac aggatacggt
     1861 gaacgacgcc gaccttctcg attttcaggt cgccgatcac ggaggatgcg cggatggtgt
     1921 ccccgatgtc aatggacgcc ggatcgatat accagacgtt cggcaggacg ggcctacgcg
     1981 gggacatggt tcatcacccc cgcgatgcct ttcatgttcg cataggtgcc cgttgcgatg
     2041 atgatgtagt caccttcgcg cgtctgttgg agcaactgtg cacgatctgc ctggtgtggt
     2101 gcgccgggcc tgtgcggatc aaccacgagc acccgatatc tagccgtgtc catattcttt
     2161 ttccttccca cagtctacgc attcgtagac gtatcgatgc tcactgacga gcacatagtc
     2221 atggtaaccg ccgagcgtgc ccggacagcc aacctcattg tcagccattc gattccgccc
     2281 gtccgatgtt cggggccgac acggacacac cgaagattcc ggacgctgtg ctgatgagag
     2341 cgttgatgaa cgcaataacc cgatctggga tggtggaacc gttggcgact tcggggaagt
     2401 agatgaaaaa cagggacgcc gcaccagaca cgcccgcgag tgcgtacata ctgtaagaca
     2461 ggatgcgccg gaacttcgtg gacgtgatcc gaaccgtctt cgggagggat tcctgatagt
     2521 cagtgcgcgg cgtcagggat gatgcgagga cgggcggg
//
LOCUS       DATMTI010000008         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_42904, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000008 DATMTI010000000
VERSION     DATMTI010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42904"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VN039_00135"
     CDS             complement(<1..578)
                     /locus_tag="VN039_00135"
                     /inference="COORDINATES: protein motif:HMM:NF042497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HWV44429.1"
                     /translation="MSSLLGISRTVQNVMTVVKRQFGDESGVQLEDSDIINWVNDAQD
                     TIVNRNRVLKAKSTTAITAGQSTVTFPNLRIIQIESVHYNGRILPNMSFVEAEQKIST
                     SDPQSSESGIPLFWYEWAGTMTLWPTPDADGSLDLYYSAFPTRVTGATDSLSLPDKYF
                     EDICRYVMQQVYEMDQDWQGSQAKQQQFDASV"
     gene            complement(575..1033)
                     /locus_tag="VN039_00140"
     CDS             complement(575..1033)
                     /locus_tag="VN039_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44430.1"
                     /translation="MTIFGDSAGTWNSEYGEFISDDHQRFAEVLKDVNPNLELAFIPR
                     KDRDESDTKPFAIVEHRPGFEPQIIRYLSVLEMERPSEILAWCAAGDLVRNRPQDVLA
                     RIEREETMQELLDLKRKEDELEDILDMGAFVGRTPLNKFKIQPKGRFIRT"
     gene            complement(1214..>2614)
                     /locus_tag="VN039_00145"
     CDS             complement(1214..>2614)
                     /locus_tag="VN039_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWV44431.1"
                     /translation="QCDGVNANVQYVPGVGTLLSVYQPWASELSSLLSAYVPEMPSKP
                     IDAAVAPLIPDTGTATGAAISGALHAALDSPALTGTPTAPTPAVGDSSGSIATTAFVG
                     AAFMHWTAPTALNPWFAALANREHEKACAVFYGTSMTEGGPMITTSRLDRWLDRLAFD
                     LHAKFPLRAPVPGSSGYIPSFYVSFPKGGSTQFGLTVPTGATIPRALAVAGPGIGARS
                     LEIAQAGDYMQFAFIGTGFDFLYNSRDVASSAATITVDNGAAVALPVPSATNTQATYQ
                     GRGLTAGSHTVRVDWTAGHVDIQGVYPYNGDEANGIHIWDAGKGSAKVSDGFTGTGAY
                     WALSNIQPALVGFEFGYNEYFGGIAPATFAANLATAVSNALAQITTPPSVLFLVWPRG
                     GGADTYTYDYQAYVDAIYAQAASTANAVVVDFSERLPSPRVNNALGFFGVDTTHPADK
                     GGAYIADTILAVLSPH"
BASE COUNT          587 a          722 c          726 g          579 t
ORIGIN      
        1 ttcaccgacg catcgaattg ctgttgttta gcctgactac cttgccagtc ctgatccatt
       61 tcatatacct gctgcataac gtaacggcaa atatcttcaa aatacttatc gggcaacgac
      121 agggaatcgg tggccccggt aactcgggtc gggaaggctg agtaatacag atcaaggcta
      181 ccgtcagcat cgggagtggg ccagagcgtc attgttccag cccactcgta ccagaaaagc
      241 ggaatacctg attctgagct ttgagggtca ctagtcgaaa ttttctgctc agcctcaaca
      301 aaactcatat tggggagaat acggccattg tagtgtaccg attcaatctg aatgatacga
      361 agattgggga acgtaaccgt agattgacct gccgtaatag ctgtagtgga cttagctttg
      421 aggacacggt tacgatttac aatcgtgtct tgcgcgtcat ttacccagtt gattatgtcg
      481 ctatcctcaa gctggacccc ggactcgtcc ccaaactgtc gcttaacaac agtcatcaca
      541 ttttgaacag tgcggctaat tcccagcaag ctactcatgt tctgataaac cgtcctttag
      601 gctgaatctt gaacttgttt aggggggtac gccctacaaa agcccccatg tcaagaatat
      661 cctctagctc atcctctttt cgtttgaggt caagtaattc ttgcatagtc tcttcgcgtt
      721 cgatgcgcgc cagcacgtct tgcggtcgat ttcgtacaag atcgcccgcc gcgcaccacg
      781 ctagaatctc agacgggcgc tccatttcca gaaccgacag atagcgaata atctggggtt
      841 caaatcctgg cctatgttct acgatagcga acggcttagt gtctgactca tcccgatcct
      901 tacgcggaat aaaagccagc tctaggttag ggtttacgtc ctttagaacc tcggcaaacc
      961 tctgatggtc atcactaata aattcgccgt attcgctatt ccatgtaccg gcgctgtctc
     1021 cgaaaattgt catagtcgca tcttagcgct cgtagccaac gtgagaggcg gtcttcagta
     1081 ttccaaactg atggtgcggg ttcgattccc gtcagcccct ctaggaaccg agcccgtggt
     1141 gtacccgaaa ctgtacccga accagtcaaa cgactccggg cgctatcagg gtggtggggc
     1201 gtacctggct ctgctagtgc ggagagagca cggcaaggat tgtgtctgcg atgtatgcac
     1261 cgcccttgtc ggctgggtgc gtggtgtcca caccgaaaaa gccgagcgcg ttgttcacac
     1321 gcggcgacgg aagccgctca ctgaaatcga cgacaaccgc attggccgtc gatgccgcct
     1381 gtgcatagat ggcgtccacg tatgcctggt aatcatacgt gtaggtatca gctccaccac
     1441 cgcgcggcca cacgaggaag agtacggacg gcggagttgt aatctgtgcg agcgcgttcg
     1501 aaacggcggt ggcgaggttc gcagcgaatg tggccggggc tattcccccg aaatattcgt
     1561 tgtagccgaa ttcgaatccc accagtgcgg gctggatatt cgagagtgcc cagtaggccc
     1621 cggtaccagt aaaaccgtcg ctgactttcg cagacccttt gcctgcatcc cagatatgga
     1681 tgccattggc ctcatcgccg ttgtatgggt agacgccttg aatatcaacg tgccctgccg
     1741 tccagtcgac gcgcacagtg tgcgagcctg cggtgagtcc acggccctga tatgtcgcct
     1801 gcgtgttcgt ggcagacggg acgggaagcg cgacggccgc accattgtcg acggtgatcg
     1861 ttgcggcaga cgatgccaca tcgcgactgt tgtagagaaa gtcaaagccg gtaccgatga
     1921 atgcgaactg catgtagtcg cccgcctggg cgatttctag cgaacgcgcc ccgataccag
     1981 ggccggctac agccaacgcg cgcgggatcg tcgctccggt ggggacggta agaccgaatt
     2041 gcgtcgaacc acctttgggg aaactgacgt aaaacgaagg gatgtatccg ctgctgccgg
     2101 ggacaggcgc gcgcagtgga aactttgcgt gtagatcgaa agcgagtcgg tccaaccatc
     2161 tatccaggcg ggaggtcgtg atcatcggcc caccctctgt catcgacgtg ccatagaaca
     2221 ctgcacatgc cttctcgtgc tcacggttgg caagcgctgc aaaccacggg ttgagagcag
     2281 ttggcgcggt ccaatgcatg aacgcggcgc ccacgaatgc tgtggtggcg atgcttcccg
     2341 acgaatcccc cacagcgggt gtcggtgcgg tcggcgtgcc cgtcagggca ggagagtcca
     2401 gcgccgcgtg gagcgccccg gagattgctg ctcccgttgc tgttccggtg tccggtatca
     2461 gaggagcaac agcagcatca ataggcttag atggcatttc aggtacataa gccgacaaga
     2521 gagaagagag ctcgcttgcc caaggctgat agacggatag cagagttcct actcccggaa
     2581 catactgcac attagcgtta accccatcac actg
//
LOCUS       DATMTI010000009         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_509634, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000009 DATMTI010000000
VERSION     DATMTI010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509634"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VN039_00150"
     CDS             <1..224
                     /locus_tag="VN039_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44432.1"
                     /translation="SPQSKIAALNLIKGSALFSRGVSLAKTYLKERSKLLAKLADTTA
                     VNTRRADFMNELKSRNGLPLFQVDARSGE"
     gene            349..>2129
                     /locus_tag="VN039_00155"
     CDS             349..>2129
                     /locus_tag="VN039_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWV44433.1"
                     /translation="MPALEPVSCCRKIQRMSTEIKVGPPVITISQGRTFIVTTTAGDI
                     RANTDEGVYALDTRFISFYRLFINRVPLKVINSSQLTFYAARHYLTNPRINSQDGLIK
                     ENTLNITIDRIVTDGVHEELEVTNYNGRKGTFLLELCIRSDFADIFEVKAQKIVQRGQ
                     TETVWDKNRRQLRTSYDNEDFHRAAIYHVTNVSDQTPVGYANVRICFQINLEHGQCWH
                     MCGDLLLEHGQETKKPQPGSCSYQQSSRSGKAGELSGKRQPSSDFGVRQSHWLDRCTR
                     VATSNNDLLRMYDQALLDMGALRIYDMDVSDEAWVPAAGVPWFVALFGRDSLIVSYQN
                     MTVSPGFARGALKRLAEYQAKERDDWLDAQPGKILHEMRFGELAEFHKIPFYPYYGTA
                     DATILYLIVLSETYRWTGDRALLKEYQQVAEHCLKWIDEYGDLDGDGFQEYKTFSSLG
                     YENVGWKDAGNAVVYADGTQVQQPKGLCTLQSYVYNAKIRMAEAFRVLGDETRAKALD
                     AQAKKLREQFNNAFWMEKEGCYAFGLDPGKKQITSVTSNAGQVLWSGIADQDKAERTA
                     TRLLQPDMWSGWGIRTLSSNNPAYNPL"
BASE COUNT          531 a          522 c          603 g          473 t
ORIGIN      
        1 attcgcccca gtcgaaaatt gctgctttaa atttgataaa ggggtccgct cttttcagtc
       61 gcggtgtttc actggcgaaa acatatttga aggagcgctc gaagctcctt gcgaagttag
      121 ctgacacaac agccgtcaat actcgacgtg ctgatttcat gaatgaactg aagtctcgca
      181 atgggttgcc gctgtttcag gtcgacgcgc ggtcgggaga ataaagggcg cttcgaatcg
      241 atcggaaggt gcaatgaaga gcccggcatc aggacacaca gtttcctcac gcaaacgagt
      301 aaacaaccct gaaggggatc atcagcgatg gatatatagg agacgcagtt gccggcgctt
      361 gagccggttt cttgctgcag gaaaatacaa cgtatgtcga cagagattaa ggttgggccg
      421 ccagtcatca cgattagcca gggacgcacg tttatcgtga ctacaaccgc aggcgacata
      481 cgagccaaca cggacgaggg cgtctatgct ttggacacac gttttatcag tttttatcgc
      541 ctctttatca accgcgttcc gctgaaggtg atcaactcga gtcaactaac gttttatgcg
      601 gctcggcatt atctgaccaa tcctcgaatc aacagtcaag acggcctgat aaaggaaaac
      661 acgcttaaca ttaccatcga caggatagtc actgacgggg ttcacgagga gctcgaagtc
      721 accaactaca acgggaggaa aggcactttt ctactggaac tttgcatccg gtcagacttc
      781 gcggacatct ttgaagtcaa agcgcagaag attgtccagc gtgggcagac cgagaccgtc
      841 tgggacaaaa atcggaggca acttcgcacg tcgtacgata acgaggactt ccaccgcgct
      901 gcaatttatc acgtcaccaa cgtttccgat cagacgccgg ttggttatgc gaatgtacgc
      961 atctgctttc aaatcaatct cgaacatggc caatgctggc atatgtgcgg ggatttgttg
     1021 ttggagcatg gtcaggagac gaaaaagccg cagcctggat cgtgcagcta tcagcagtcg
     1081 agcagatcag gtaaggcagg cgagctgtct ggcaaacgcc agcccagctc cgattttggt
     1141 gtgcgacaga gccactggtt agaccggtgc acgcgagtgg cgacctcgaa taacgacctg
     1201 ctgcgcatgt acgaccaagc cctgctggat atgggcgctt tgcgcattta cgatatggat
     1261 gtctccgatg aagcgtgggt gcccgcggct ggggtgccgt ggttcgtggc acttttcggg
     1321 cgggatagtt tgatcgtctc gtatcagaac atgacggtgt caccggggtt cgcccgtggc
     1381 gcactgaagc gactggcgga gtaccaggct aaggagcgcg acgattggct ggatgctcaa
     1441 ccgggcaaga tcctgcacga aatgcggttc ggcgaattag cggagttcca taaaattcct
     1501 ttttaccctt attacggcac cgctgatgcc acgattctgt atctgatcgt actgtcggaa
     1561 acctatcggt ggacgggcga tcgcgcttta ctcaaagaat accagcaggt cgcggaacac
     1621 tgtctcaaat ggatcgacga atacggcgac ttggatggcg acggattcca ggagtacaaa
     1681 accttttcct cgttaggata tgaaaatgtt gggtggaagg atgctggtaa cgcggtggtg
     1741 tatgctgatg gcacccaggt tcagcagccg aagggtcttt gcacacttca aagttatgtg
     1801 tacaacgcga aaatccgcat ggccgaagcc tttcgcgttt taggcgacga gactcgggcg
     1861 aaagctttgg atgcgcaggc aaagaagctg cgagagcagt tcaataacgc attttggatg
     1921 gagaaagaag gctgttatgc ttttggcctc gatcccggca agaagcagat cacctctgtc
     1981 acatcgaacg cggggcaggt cctctggagc gggattgcag atcaggataa agcggaacgt
     2041 accgctacac ggcttttgca acctgacatg tggagcggct ggggaattcg aacgctctcc
     2101 agcaacaatc cggcatacaa tcctctcga
//
LOCUS       DATMTI010000010         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_721764, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000010 DATMTI010000000
VERSION     DATMTI010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721764"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VN039_00160"
     CDS             complement(<1..1424)
                     /locus_tag="VN039_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV44434.1"
                     /translation="MGMTRIIIVGGGFAGVKCAKTLRKRLSTNTCEIVLFSRENNMVF
                     YPLLAEVAGAAINPLTVTVPLRQMLPEVRCRTEEVRQIDLATSEVEYEGYDGRSSRMA
                     FDHAVLACGAAVNLTMVPGMADHAFPLKSVGDAVALRFHVMEQLEKAEVCDDPERRRW
                     YLSFVVVGGGFTGVEAAGEINDLIKASTRFYGNFTVSDVTVTLVHSRNQILPEVSPSL
                     REFARIKMEEAGIHMVLNARAVSATAEGVELHDGRRLRGATIVCTIGTTAPLLVHRLK
                     VSKEQGRLLTDPDMRVRGISNLWAIGDCAHIVNAYDGDVSPTTGQFAERQGGQAAENI
                     IRALQGESTRPFLFKPLGQLCGIGERKAVAEMFGVRLSGFPAWWLWRSVYLLKSPSWS
                     RRVKVAFEWTWELLFPRDLAYPRIARTERIARAHYRPGDFIFVEGEPAVNFYVIEQGE
                     VEAMRRDLTGQPKLMAVFGPGEFFG"
BASE COUNT          338 a          513 c          408 g          319 t
ORIGIN      
        1 ccgaaaaact ctcccggtcc aaacaccgcc atgagtttgg gttgtccggt caggtcacgc
       61 cgcatggcct cgacctcacc ctgttcgatt acgtaaaaat tcaccgccgg ctcgccttcg
      121 acaaagataa agtcacccgg acgatagtgc gctcgcgcaa ttcgttcggt tcgagcgatc
      181 ctgggatagg ccaggtcgcg tggaaacagc aattcccagg tccactcaaa cgcaaccttg
      241 actcggcgtg accaggacgg cgatttcagc agatacacgc ttcgccacaa ccaccaagcc
      301 ggaaagccgg agagtcgtac accgaacatt tctgcgaccg ccttcctctc tcctatcccg
      361 cagagttgtc ccagcggctt gaataaaaac ggtctggtgg attcaccctg tagagcacga
      421 atgatgttct ccgccgcttg ccccccctgc cgttcggcaa actggccggt cgtcggtgag
      481 acatcgccgt cataggcatt gacgatgtgg gcacagtcgc cgatcgccca gagattcgat
      541 atcccgcgca ctcgcatatc aggatcggtg agcagtcggc cttgttcctt ggataccttg
      601 agccggtgca ccagaagcgg tgccgtggta ccgatcgtac agacaattgt ggcgccacgg
      661 agccttcgac cgtcatgcag ctcgacaccc tcggctgtgg ccgataccgc gcgcgcattc
      721 agcaccatgt gaatgccggc ctcttccatc ttgatacgag cgaactcgcg cagtgacggg
      781 ctcacttccg gcagaatctg attgcgactg tggaccaacg tcacagtcac gtcactgacg
      841 gtgaagttgc cgtaaaaccg tgtgctggct ttgatcaagt cattgatctc gcctgccgcc
      901 tcgacaccgg taaaaccgcc gccgaccacc acgaacgaga gataccaccg tcggcgctcc
      961 gggtcgtcgc agacttcggc tttttccagt tgctccatca cgtggaacct caatgccacg
     1021 gcatcgccaa ccgatttcaa cgggaacgca tggtcggcca tgccgggcac catggtgaga
     1081 ttgactgcag ctccgcaagc cagcacggca tggtcgaacg ccatgcggct cgaccgacca
     1141 tcgtaccctt catattccac ttcggatgtt gcaaggtcga tctgccggac ctcctccgtt
     1201 cggcaccgta cttcaggcag catctggcgc aacggcacag tcaccgtcag ggggttgatc
     1261 gcggcaccgg ccacttccgc cagcaatggg tagaacacca tgttgttttc ccggctgaat
     1321 agcacgatct cacaggtgtt tgttgacagc ctcttccgca gagtctttgc gcacttcacc
     1381 ccggcaaatc cgccgcccac gatgatgatc cgcgtcatgc ccatggcgtt aacacccaga
     1441 ctgtctggag ctcgaacaag cagacaacca ccttgcgctc acgttcgaac cgttggctaa
     1501 acgcagcacg actgtacgca aacccattcg gagagaattc gtccgaaagg cctttcaacg
     1561 gctcgttgac ccaattca
//
LOCUS       DATMTI010000011         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_382303, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000011 DATMTI010000000
VERSION     DATMTI010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382303"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VN039_00165"
     CDS             complement(<1..192)
                     /locus_tag="VN039_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWV44435.1"
                     /translation="MRRVFGLETEYGITLSGAETVDVVAESIELVRRYTDHGALMKWD
                     YDLEDPHLDARGFRARELMQ"
     gene            complement(366..1610)
                     /locus_tag="VN039_00170"
     CDS             complement(366..1610)
                     /locus_tag="VN039_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV44436.1"
                     /translation="MGKDRLRVGSEHGLHSMVLQRSSELAHSNLKSGDEVRVDSNYRM
                     ALEMLSSTKSDEYYLDTVPELPWEKVGGQEAALQAIKDAIELPLLHPDLFKKFQHATP
                     KGFLLYGPPGCGKTLIGKATAFNLTKQLGQQTGTEMREYFMHVKGPEILNMWVGESER
                     MVREIFATAREKRKEGFMPFLCIDEAESILGTRRASRHSNILSTLVPMFCSEMDGIDS
                     LNDVVIILASNRADLIDPAILRPGRIDRKIKVNRPDKTGTREIYRIYLTDSLPYDGVL
                     AKEADGIGASIERLIERFVDWQFARRDENKFLEATLRSGRKETLYRSDLISGAIIASI
                     IERAKAIAIKRAIGTPQDEGIRETDLQLAFNAEYAENDIFPATDITEDWLKLIDYDPE
                     NVVKISPVRPVSDARVRATSVI"
BASE COUNT          341 a          441 c          434 g          402 t
ORIGIN      
        1 ctgcattaat tcccgggcgc gaaacccgcg ggcatcgagg tgaggatctt cgagatcata
       61 atcccatttc atcaaggcgc cgtgatcggt gtaacggcgc actagttcga tcgattccgc
      121 caccacatcc acggtttcgg caccagacag cgtgatgcca tactccgtct ccagcccaaa
      181 gacgcgtctc attgtcagcc cgctttgccg cagtaacgaa gttgcaggtg atcttccacg
      241 gggatactca ccagatgcat aatcgagttt gccaatgtag caggattgct gggagaagtg
      301 tcggtccggc catatacatg ggaagcgagc tgctttcgct tcgtttcaac gtgattgcac
      361 tcgcattaga tcaccgaggt tgcgcgcacg cgggcatccg aaactggcct aaccggtgaa
      421 atcttcacga cgttttcagg atcataatcg attagcttga gccaatcttc ggtaatgtct
      481 gtggccggga aaatatcgtt ctctgcgtac tcggcattaa aggcgagttg caggtccgtt
      541 tcccgaattc cctcgtcctg tggagtcccg atggcgcgtt taatcgcgat cgctttggcg
      601 cgctctatga tcgaggcaat gatcgcgccg ctgattaaat cgctgcggta aagtgtttct
      661 ttgcgaccgc tgcgcagggt cgcttctaaa aatttgtttt cgtcacgccg ggcgaattgc
      721 cagtcaacaa accgctcgat caggcgttcg atagacgcgc cgattccgtc agcttccttc
      781 gccagcacgc cgtcgtacgg cagactgtca gtgagataga tccgataaat ctcgcgcgtt
      841 ccggtcttat cgggacgatt gactttgatc ttccggtcga tacggccggg acgcaaaatt
      901 gcggggtcga tcaggtccgc gcggttcgac gccagaatga tcacgacgtc gttgagcgaa
      961 tcgattccgt ccatctccga gcagaacatc ggtaccagtg tggaaagaat atttgaatgt
     1021 ctcgatgcgc gtcgtgtgcc aaggatcgat tcggcttcat cgatacagag gaacggcatg
     1081 aaaccttcct tgcgcttttc tcgcgccgtg gcgaaaatct cgcgcaccat tcgttccgat
     1141 tcgcccaccc acatgttgag gatctcgggt cccttcacgt gcatgaaata ttcgcgcatc
     1201 tctgtgccgg tttgttggcc gagttgcttc gtcaaattga atgcagtcgc tttgccgatg
     1261 agcgtctttc cgcaaccggg cggtccgtaa aggaggaaac ctttgggcgt agcgtgttga
     1321 aattttttga aaagatcggg atgcaaaagc ggtaactcga ttgcgtcctt gattgcttgc
     1381 agcgccgctt cctggccgcc gactttttcc caaggcaact ccggcaccgt gtcgaggtaa
     1441 tattcgtccg atttagtgct cgagagcatc tccaacgcca tgcgataatt ggagtcaacg
     1501 cgaacttcat cgcccgattt caaattcgag tgagcgagtt ctgaggagcg ttgcagcacc
     1561 atcgaatgca atccgtgctc ggaacccacg cgtaatcggt ctttgcctat gacctcgg
//
LOCUS       DATMTI010000012         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_340002, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000012 DATMTI010000000
VERSION     DATMTI010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340002"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VN039_00175"
     CDS             <1..684
                     /locus_tag="VN039_00175"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV44437.1"
                     /translation="ALAKEPGSKVFISLAEEYGKAGMWEEAVAVLEDGLKTYPGFITA
                     MVALGRAYEQLNQPVKAKAILEEAIKSSPDNLRAHRILAKLYGAQGAKEAAIRSCNVI
                     LSINPHDQEALSLRSGLDVPTTHGAAQSQGQLDEQPVKASSLDSVSLRGKGMTSILGD
                     TTLPTKDKANGLSRNLPRAEEKPVPTGGSTAQTSQKTRSPVIVQLEQWLKSVRTRRQD
                     PQASSRSRS"
     gene            787..1260
                     /gene="aroQ"
                     /locus_tag="VN039_00180"
     CDS             787..1260
                     /gene="aroQ"
                     /locus_tag="VN039_00180"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWV44438.1"
                     /translation="MLRILVLHGPNLNLLGHREESIYGTVTLDTIDASLIKLGSELGA
                     ELVIRQSNLEGELVTWIQEAQRGYQGIIINPAAYTHTSIAIRDALAAVDLPAIEVHLS
                     NIYRREEFRHHSYVSGVVLAQIAGFGATGYLLALRGLCEQLSAAGSKGFSGGRTR"
     gene            1347..1907
                     /gene="efp"
                     /locus_tag="VN039_00185"
     CDS             1347..1907
                     /gene="efp"
                     /locus_tag="VN039_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWV44439.1"
                     /translation="MISTVDFRSGVRLMVEGEPFYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGERFDEPDLEERDMQFLYASGDSYTLMDTETYEQLTFERSQLGGNA
                     DLLKENMIVKILIYEHRPIAVELPNFIELRVVDAEPGVRGDTASGGTKPAVVETGATI
                     KVPLYLEVGTVIRIDTRTRSYVERVR"
     gene            1904..2467
                     /gene="accB"
                     /locus_tag="VN039_00190"
     CDS             1904..2467
                     /gene="accB"
                     /locus_tag="VN039_00190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HWV44440.1"
                     /translation="MNRRRSVHSKAKTRRIVLPETANETRHEASLTGGSTKQIQELID
                     LLRRNNLTELEFERQGIRIRVRHELVARPLATSAQESVPILAQQPMHSASATASVSDA
                     STGFVTVTSPIVGTFYRSPSPDADPYVDEGESVKKGQVLCIVEAMKLMNEIESEVDGR
                     LVKILVENTKSVEYGQALFLIDPKAAS"
     gene            2511..>2942
                     /locus_tag="VN039_00195"
     CDS             2511..>2942
                     /locus_tag="VN039_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWV44441.1"
                     /translation="MFKKVLIANRGEIALRVIRACKELGIKTVAIHSEADALALHVRA
                     ADEKVCVGPAESALSYRNIPNVLSAAEITGVDAIHPGYGFLSENAHFAEVCESIGVKF
                     IGPSSENIAMMGDKAKAREIVAKRGLPVTPGSPGELRSEEDA"
BASE COUNT          723 a          732 c          831 g          656 t
ORIGIN      
        1 gccttagcta aggagccggg gtccaaggta tttatttctc tggcagaaga atacggcaaa
       61 gccggcatgt gggaagaggc cgttgccgtc cttgaagatg ggttgaaaac ctatccaggt
      121 ttcatcaccg ccatggtggc gttaggtcgg gcatatgagc agctgaacca accggtcaag
      181 gctaaggcca ttctcgaaga agccattaaa tcaagtcctg ataatcttcg tgcacatcga
      241 atattggcca agctctatgg agctcaaggg gccaaagaag cggctatccg atcgtgcaat
      301 gtcattttat ccataaaccc acacgaccaa gaagccttat ctcttcgttc agggctcgac
      361 gtaccgacga ctcatggcgc ggcgcaatcg caaggacaat tggacgagca accggtgaaa
      421 gcttcaagcc ttgactctgt cagccttcgt ggaaagggta tgacatccat cttaggcgat
      481 acaacgctgc caacgaaaga taaagccaac ggcttgtcta ggaacctacc tcgcgcagaa
      541 gaaaaacctg ttcctacggg cggttccaca gcccaaacct cacagaaaac gagaagcccg
      601 gtgatcgtac agcttgagca gtggctcaaa tcggtccgga cacgtcgaca agatccccaa
      661 gcctcttctc gctcccgctc atgaacttcc tcatgacttc aatggtttga ccctctgaga
      721 acggactgct agaatgccgc ccttgccgtg gtgatgtaag caggtgtaaa gggaggtatc
      781 gcacacatgt tgcgcatcct ggtccttcac ggaccaaatc tgaacctgtt gggtcatcga
      841 gaggaatcca tttatgggac ggtgaccctt gatacgatcg atgcatcgct gataaagctg
      901 ggtagtgaac ttggggctga gcttgttatt cgccagtcga atcttgaagg agaattggtg
      961 acctggattc aagaagcgca gcgaggctac caaggcatta tcattaatcc agcggcctat
     1021 actcacacca gcatcgcgat acgcgatgcc ctggccgccg ttgatttgcc tgccatcgag
     1081 gtccatctgt caaacatcta tcggcgtgaa gaattcaggc accattcata tgtgtcgggg
     1141 gtcgtgctgg cacagatcgc cgggttcggt gcaaccgggt atctattggc cttgcgtgga
     1201 ctgtgcgaac aactatctgc cgccggatcg aaggggtttt ctggtgggcg tactcggtaa
     1261 gcggaacgaa ggcaggggca caccgagagt atcaccagcc ggttgtttgc ttaagttgtt
     1321 tgcttaaaaa gagaagggag ttgcgagtga tttccacagt ggattttcgt agcggtgtac
     1381 gcttgatggt cgaaggcgaa cccttttata tcgtcgagtt tcagcatgta aagccaggca
     1441 aaggtggtgc cttcgtacgt acgaagttga aaagttatct ctccggaaac gtactggacc
     1501 gaacgtttcg ttcaggagaa cggtttgatg agcccgatct ggaagagcgc gatatgcagt
     1561 tcctgtacgc atccggagat tcctataccc tcatggatac agagacgtac gagcaattga
     1621 cgtttgagag gagtcagttg ggagggaacg cagatttatt gaaggaaaat atgatcgtca
     1681 agatcctcat ctacgagcat cgcccgattg ccgtcgagtt gccgaacttc atcgagctca
     1741 gggtcgtcga tgcggagcca ggcgtacggg gagacactgc ctctggagga accaagccgg
     1801 cggtcgtgga aacgggagcg acgatcaaag ttccgcttta tttggaagtg ggcaccgtca
     1861 tccggatcga tactcgcacc agatcatatg tggagcgtgt tcggtgaacc gtcgtcgatc
     1921 tgttcattcc aaggccaaga ctcgtcggat tgtgttgccg gagactgcga acgagacgag
     1981 acatgaagcc tcgctcacgg ggggatccac caagcaaatc caagaactca tcgatctgct
     2041 tcggcgcaac aatttgaccg aacttgagtt tgaacgacaa ggcatccgta tccgtgtccg
     2101 tcatgaactg gtagccagac cgctcgcgac ttcagcgcaa gagtctgtcc caatcctggc
     2161 tcaacaaccg atgcattccg cttcggccac ggcatcagtg tcggacgcga gtacgggttt
     2221 tgtgaccgtc acttccccca tcgtcggcac gttttaccga tctccctcac cggacgccga
     2281 tccgtacgtt gacgaggggg agtccgtgaa aaaggggcaa gtgctgtgca tcgtcgaagc
     2341 aatgaaactc atgaacgaaa ttgaatcgga agtggacggt cgtcttgtca agatcttggt
     2401 ggaaaataca aagtcagtag aatatggtca ggcactgttc cttatcgacc ccaaagccgc
     2461 ttcataggtc attctaaagg ctgctctcgc catcgtatcg gagccgagac atgtttaaga
     2521 aagttctgat tgccaatcgc ggagaaatcg cgttgcgggt gatccgcgct tgtaaggaac
     2581 tcggcatcaa gaccgtggcc attcattctg aagcggatgc gcttgcactg cacgttcgag
     2641 cggccgacga aaaggtgtgt gtcgggccgg ccgaatcggc attgagttac cgaaatattc
     2701 ccaacgtttt gagtgccgct gagattacgg gagtcgatgc catccatccg gggtacggat
     2761 ttttatcgga gaatgcccat tttgcggaag tctgtgagtc aatcggcgtg aagtttatcg
     2821 gaccgagctc agaaaatatc gccatgatgg gggataaagc gaaggcgcgt gaaatcgtgg
     2881 cgaagcgagg actcccggtc acgcccggga gtccaggcga attgcgcagc gaagaggatg
     2941 cg
//
LOCUS       DATMTI010000013         4870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_636999, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000013 DATMTI010000000
VERSION     DATMTI010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636999"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1277
                     /locus_tag="VN039_00200"
     CDS             117..1277
                     /locus_tag="VN039_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV44442.1"
                     /translation="MRSLLFGLMVMESGCDRAPSDVVASKPPAAISAPGFITLSAEES
                     SRVGLVVQPVTRSDFRTHRDFPAIVQPNQRNMAEITTLVRGRVIEVYADFGQEVKANA
                     PLAILYSSELGLAQSAYLKAKAKLHVAEQAYSRAKFLLREQVIGEAEAQRRQAELLSN
                     QAEANESRDRLKLLGMDEEEFGRLDRSRQIRSVVSIVAPFAGRIIGRKLTRGEVVETT
                     ENLFVIADLSEVWVQANIPEKDIPFVHAIHASGGTQVEVRINAYPKEVFKGTITYVGD
                     VLDPVTRTMQLRIELPNRDGRLKPEMFATIRLFSEAQLDRLAVPEAALQRDQGRTFVF
                     VQRNANEYELREVHVGESNGTVIAILSGLNEGEPVVTHGAFVLKSELLKKPV"
     gene            1277..4504
                     /locus_tag="VN039_00205"
     CDS             1277..4504
                     /locus_tag="VN039_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWV44443.1"
                     /translation="MVSRLLDMSLRQRMLVIICALMIGAGGIYAFRTIPIDAFPDVTS
                     VLVQVVTKAPGLSPAEVERLVTYPIELQLTGVPSLTEMRSLTKVGLSLITIVFDDSMD
                     INLARQLVLERLLEVEEQLPPGAEPMLVPNSTGLGEVFQYYLESPRGSSSDADAEHQS
                     LIRQRTIQDWVIRPLLKSTPEVIDVNSMGGYVKQYQVLVEPGMLRKYSLTLRDVFDAV
                     SRNNANAGGNILEKHDEKYIVRGIGLIRSLQDIERIVVKETGGTPVFVGDVAQVLIDH
                     AVRHGATVLNGDREVVSGIVLMLRGGNARDVVEGIKARIEDIHAKDLLPDGLRIVPFY
                     DRIELITEALNTVYKSLAEGVVLVTVVLLLFLGNIRSALIVVGTLVLAPLATFIVMGQ
                     IGLTANLMSLGGLAIAIGMIVDGSVVVVENVYRHLSHHSAATIPRLQLVTNAVKEVGQ
                     PVVFGILIIILVFLPLLSLHGMEGKMFKPLAYTIMIALMVSLVLSLTLSPVLCSVALR
                     QGSEEDPWIVRQAKGLYAPTLRWALGHRTVVLVMAIGALIGALALVPTLGTEFIPILN
                     EGSVAPQTIRLPSVSLPASIEIEKRMQQAIMEFPEVEMVVSKIGRTELGNDPQEPNES
                     DPVVRLRPLDQWTTAKTMPELMQKFRERLSGVSGATFLISQPIQQRVDELISGVRTEA
                     TVKLFGDDLEMLRNKAQEIAEVLGSVQGVKDIKVEQLFGQPYLTIDIDRGKIARHGIN
                     VSDVREIITTAIGGQAATRVYEGQQRFDLVVRFPEQYRDSVETISNILLSDQTGALIP
                     LADLGTVQLEEGPGRISRDQLQRYVSIGFNTMGRDIGSLVAEAQQKITERVALPAGYR
                     ITWGGSFENMERAMAKLQVIVPITIGLIFFLLYSTFNSLRQATLIILNLPFALIGGIV
                     ALWLTGEYLSVPASVGFINLFGVAVLNGIVLVSYMNKLREDGHSLDDAVTSGALLRLR
                     PVLMTALVALLGLVPLAFANGIGSEVQRPLAIVVIGGLVSSTLLTLIMLPVLYRWLEG
                     RSKDSMHRGEPQTGSTVDGHHTVGEASHANGEPRFTRHPGEIPST"
     gene            complement(4639..>4870)
                     /locus_tag="VN039_00210"
     CDS             complement(4639..>4870)
                     /locus_tag="VN039_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44444.1"
                     /translation="FSDVQQRYDEQMRTHFSFNERPQRSEVVFHRKFLDDCKNLNEIM
                     EKSILPLLLANPGKSIIVGEMGGLSLEDRGKN"
BASE COUNT          998 a         1300 c         1464 g         1108 t
ORIGIN      
        1 caagcacggc acgacctctc tgtctcgctg gctcggcttg aacaggccgt cggggggaca
       61 ttgtgaaaat cgttttcgat tcaatgcaag ttccatcgtt atccgtcagt ctgggcatgc
      121 gtagtcttct gttcggtttg atggtgatgg aatcaggctg tgatagggcg ccgagcgatg
      181 tggtggccag taagccgcct gccgcaatct cagctccagg tttcattacg ctgtcggcgg
      241 aggagtcgtc acgggtggga ctcgtggtcc agccggtgac gcgcagcgat tttcgaacac
      301 accgcgattt ccccgcgatc gtacagccca atcaacggaa catggcggag attacgacct
      361 tggttcgagg tcgagtcatc gaggtgtatg cagacttcgg gcaagaagtg aaagccaatg
      421 ctcccttggc catcctctac agcagcgagc tcgggcttgc ccaatctgca tatctcaagg
      481 ccaaggccaa gctccacgtc gctgagcagg cgtatagccg ggctaaattc ctcctgcggg
      541 agcaggtcat cggcgaagcg gaagcccaac gccggcaggc ggagctgctg agcaatcagg
      601 cggaggccaa tgagtcacgt gatcgcttga aattgcttgg catggacgag gaagagttcg
      661 gccgtctcga ccgcagtcgg cagattcgat ccgtcgtgtc gatcgtggcc cccttcgccg
      721 gtcgcatcat cggacgtaag ttgacacgag gggaagttgt cgagacgacc gagaatttat
      781 tcgtgattgc cgatctttcg gaggtctggg ttcaagccaa tattccggag aaggacattc
      841 cgttcgtcca tgcgatccat gcatcgggtg gtacgcaggt ggaggtgcgg atcaacgcct
      901 atccgaagga agtcttcaaa ggcacgatca cctacgtcgg cgatgtattg gatcctgtca
      961 cacgcaccat gcagctcagg attgaattgc cgaatcggga tgggcgactc aaaccggaaa
     1021 tgttcgccac gattcggctc ttttccgaag cacaactcga tcgcctggcg gtgccggagg
     1081 ccgcgctgca acgcgatcag gggcgcacct tcgtcttcgt gcaacgcaac gcgaatgagt
     1141 acgagttgcg ggaggtgcat gtcggtgaat ccaatgggac ggtcattgcg atcctcagtg
     1201 gcctgaacga gggagagccg gtcgtgacgc acggcgcgtt tgtcctgaag tccgagttgc
     1261 tgaagaaacc cgtctgatgg tctcccgcct cctcgacatg tccttgcgtc agcggatgct
     1321 cgtcatcatc tgcgcgctca tgatcggggc cggagggatt tatgcctttc ggaccattcc
     1381 gatcgatgcc tttcccgatg tgaccagcgt tctggtacag gtcgtgacga aggccccggg
     1441 cctctcgccg gccgaagtcg agcgtctggt gacctatccg atcgagctgc agctcaccgg
     1501 tgtgccgtct ctcacggaaa tgcgttccct cacgaaggtc ggtctttcgc ttatcacgat
     1561 cgtgttcgat gactcgatgg acattaacct ggcccgtcaa ttggtgctcg agcgattgct
     1621 cgaagtcgag gaacagcttc ctccgggagc cgagccgatg ctggtgccga acagcacagg
     1681 gttgggagag gtgtttcaat attacctgga gagccctcgg ggttcctcgt ccgatgccga
     1741 cgcggaacat caaagtttga tccgacagcg cacgattcaa gactgggtga tccggcccct
     1801 cttgaaaagc acaccggagg tcatcgacgt caattcgatg ggcgggtacg tcaagcagta
     1861 tcaggtgttg gtcgagccgg gcatgctgcg gaaatacagc ctgactcttc gcgacgtctt
     1921 cgatgctgtc tccagaaaca atgccaacgc cggcgggaat atcctcgaaa aacatgatga
     1981 aaagtacatc gtgcgaggga tagggctgat ccgctcgctc caggatatcg aacgcatcgt
     2041 ggttaaggag accggcggaa ctccggtgtt cgtcggagat gtagctcaag tgctcatcga
     2101 ccacgcggtg cggcacggcg cgacggtgct gaacggtgac cgcgaagtcg tgagcggaat
     2161 cgtgcttatg ttgcggggag gcaacgcgcg ggatgtggtc gaagggatca aggctcgcat
     2221 tgaggacatc catgccaaag acttgctgcc ggacggttta cggatcgtcc cgttttatga
     2281 ccgcatcgaa ctgatcactg aggcgttgaa tacggtctac aaatcattgg cggaaggtgt
     2341 ggtgctcgtg accgtcgtgt tgctcctttt tctggggaac attcgcagcg cgttgatcgt
     2401 cgtcggcacg ctcgtactgg ccccgttggc cacatttatt gtgatggggc aaatcgggtt
     2461 gaccgccaat ctgatgtcgc taggagggct ggcgatcgcg atcgggatga tcgtcgatgg
     2521 ttcggtcgtc gtcgtcgaga acgtgtaccg ccatctttca caccattctg ccgccaccat
     2581 accgaggcta caactcgtca cgaacgcggt gaaagaagtg gggcagccgg tagtattcgg
     2641 cattctcatc attattcttg tctttctgcc cctgctgtct ctgcacggca tggaagggaa
     2701 gatgttcaag ccgctggcct ataccatcat gatcgcactg atggtgtcgc ttgtgctgtc
     2761 gctcaccctg tctccggtac tctgttcggt agcgctgagg caagggagcg aagaagatcc
     2821 ttggatcgtc agacaagcca agggtctcta tgcgccgacg cttcgatggg ccctgggaca
     2881 ccgaacggtg gtgctggtga tggccatcgg cgccctgatt ggcgccttgg ctctggttcc
     2941 gactctcggc accgagttta ttcctattct caatgaagga tcagtcgctc ctcaaaccat
     3001 tcggctgccc agcgtgtcgc ttcccgcttc catcgagatc gaaaagcgca tgcagcaggc
     3061 gatcatggag tttccggaag tagagatggt tgtctcgaag atcggccgaa ctgagttagg
     3121 gaatgatccc caggagccga acgagagcga tccggtcgtg cggcttcgcc cgctggacca
     3181 atggacaacg gcgaagacca tgcccgaatt gatgcagaag tttcgcgagc gattgagcgg
     3241 cgtttccgga gcgacattcc ttatcagcca gcccatccag caacgggtcg atgagttgat
     3301 ttccggtgtc cgcacagaag cgaccgtgaa gctgttcggc gatgacctgg agatgttgcg
     3361 gaacaaggct caggaaatcg ccgaggtgtt agggagcgtg caaggggtga aagatatcaa
     3421 agttgagcaa ttattcgggc aaccgtatct caccatcgat atcgatcgcg gtaaaatcgc
     3481 ccgtcatggc atcaacgtct ccgacgtgcg ggagattatc accactgcca tcgggggaca
     3541 ggcggcgact cgggtctacg aaggacagca acgattcgat ttggtcgtgc ggttcccgga
     3601 acagtaccgg gacagcgtcg aaaccatcag taatattcta ctcagcgatc aaaccggcgc
     3661 gctgattcct ctggcggacc tcggaaccgt tcagttggag gaaggcccgg gtcggatcag
     3721 ccgcgatcag cttcaacggt atgtgtcgat tggcttcaat acgatgggac gagatatcgg
     3781 gagccttgtg gcagaagcgc agcaaaagat caccgaacga gtcgcgcttc ccgccggcta
     3841 tcgaattacc tggggcggat catttgagaa catggaacgg gcaatggcaa agctgcaggt
     3901 catcgttccg atcacgattg ggctgatctt tttcttgttg tattcaacct tcaactctct
     3961 ccgtcaggca accctgatta tcttgaatct gccctttgcg ttgatcggag gcattgtcgc
     4021 cctttggttg acaggagaat atctcagcgt gccggcgtcg gtcggcttca tcaatctctt
     4081 cggtgtggcg gtgttgaacg ggattgtgct cgtctcctat atgaataaac ttcgagagga
     4141 cggccatagt ttggacgatg ccgtcacctc gggcgcgctg cttcgactac gccccgtctt
     4201 gatgacggcg ttggttgcgc tgctggggct cgtcccgctc gcgtttgcca acgggatcgg
     4261 atcggaggtg cagcgtccgt tggcgatcgt cgtcatcggc ggcctcgtga gttcgacatt
     4321 gttgaccttg atcatgctgc cggtcctcta tcggtggctc gaaggccgct cgaaggattc
     4381 gatgcatcga ggggaaccac aaaccggatc gacagttgat ggacaccaca cagtgggcga
     4441 agcttctcat gctaacggtg aaccacgctt cactcgccac cccggcgaga taccatcgac
     4501 gtgatgggtg aatcgactga gaagggaccg gccttcttat gggtaaggta ttcatcgaga
     4561 tggtcaggag gccagccgcg cgggtttcag ttcatgcctc ttcagtggtt gtgtgctctt
     4621 ccggtggaca gtaaaccttc agtttttccc tctgtcttca aggctgagcc ctcccatctc
     4681 tcctacaatg attgatttcc ctggattggc gaggagcaac ggcaggatac ttttttccat
     4741 tatctcatta aggttcttac aatcgtccag aaactttcgg tggaatacga cctcggagcg
     4801 ttgcggtctc tcgttaaatg aaaaatgggt ccgcatctgt tcatcgtatc tctgctggac
     4861 atcggagaag
//
LOCUS       DATMTI010000014         5105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_764282, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000014 DATMTI010000000
VERSION     DATMTI010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764282"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VN039_00215"
     CDS             <1..1043
                     /locus_tag="VN039_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWV44445.1"
                     /translation="IRKVDRVTGVISTVAGMSVEDNMCSAVEGTASAQATDEDPLADG
                     VDKTGQAKFTQQADLSGTVRYWANQSVTTTKRYGGDGKTAVHAQLNFPTAVSVDREGN
                     LYIADTMNHRVRMVDSETGVISTLAGTGQARFSGDGGPANKAALNEPAALAVDEAGRL
                     YIADQSNHRVRVVDLKAGVIRTVAGTGIAAYDGDGKPAVDTALAGPSGLALAGNHLYI
                     ADTFNGRVRCVDLSSGLMTMVAGDGGVYRYESPSDAPSPSLSRPTGIALDQAGRLFLT
                     DSDNHLIRQWDWSSGTAFCVAGQGLPSYSGDGGVAREASLCYPFGIVADRDGMLLVAD
                     TFNHRIRVLALE"
     gene            1051..2073
                     /locus_tag="VN039_00220"
     CDS             1051..2073
                     /locus_tag="VN039_00220"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWV44446.1"
                     /translation="MITKKPGYTVAILGATGAVGKETLEILEERKFPLTSLRLFASKR
                     SAGEVMTCQGKEWKVEELMESSSFEGVDLAFISATDAISKDYGQRLGAAGIVVIDDSA
                     VFRMDDQVPLVVPEVNAAALGAMPRGIVSIPNCTTTPLVMVLKPLHDAVGVKRVVVTT
                     FQSVSGTGAAAMDELMDQTKDLLAFRDVTTKVYPYQIAFNLLPHIGSFNEGGDCSEEV
                     KIAKETRKILDAPSLRVTATTVRVPVLRCHSEAINVELERPLNANEARAALAAMPGVI
                     VYDDPVKKLYPMPLDVTGKDEVYIGRVREDESIANGLNLWVVSDNLRKGAALNAIQIA
                     ECLVNV"
     gene            2125..2382
                     /locus_tag="VN039_00225"
     CDS             2125..2382
                     /locus_tag="VN039_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWV44447.1"
                     /translation="MIPEWTTFGKVLIGIGIGIAALGFLLVVVDRIPGLGNAFTWLGK
                     LPGDISIKRDNFSFYFPIATSIVLSVLLSLLFYLVGWFFRR"
     gene            2379..3602
                     /locus_tag="VN039_00230"
     CDS             2379..3602
                     /locus_tag="VN039_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HWV44448.1"
                     /translation="MIAAGLLAGTAGLGLSLGSVMIPEVHAAQSIRVLLAPDVQQLEV
                     HTDQTIWMTDEHDQASSYRSVLRIKVRGHALILNGKPVVSERLTLRAGNHDLKLRLNQ
                     NGNRTALHAGDEKGVLQVSGLIQLVRRGKGLLLINHVDLEEYVKGVVPAEVNPAWHLE
                     MLKVQAVATRTYALYQHMLSSTRDYDVVSGIQDQVYRGRQGIDARVVEAVESTRGLVV
                     TYQGAPIYAAFSSTAAGITEDAMIVWSKDLPYLKGVECPFDIESPHYQWKASLKIDTL
                     EKNLRQQGFTVGTISAIMPLAYSRAGRVATLRIVHSSGELILRGEDLRKAVGYTVVPS
                     TQFAVESVGQDVVISGYGAGHAVGLCQWGAKELAELGYSFSSILRYYYPGTELQDASL
                     TQAPSVPSPLSVPAS"
     gene            3602..4621
                     /gene="queA"
                     /locus_tag="VN039_00235"
     CDS             3602..4621
                     /gene="queA"
                     /locus_tag="VN039_00235"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWV44449.1"
                     /translation="MQLSDFDFPFDSLLIASQPILPRDRARLLIVDRATRQLVHRQVV
                     DLPELLQSGDLLVVNDTRVRAARVPGIKRPTGTPVEVLFVKDLGDSRWEIMVKGSFRV
                     GQVIEFDQRSCATIVKRDAIGTEVTIESPAPVTQLFEARGIMPLPPYIKRTPTQEDHQ
                     WYQTVFAKHEGAIAAPTAGLHFTEKLFQRLREAAIKVATVTLHVGPGTFKPVTTERIE
                     DHQMGTERFTISEDAAKAIRETKRAGGRVVAVGTTVVRTLETVAMEKGEIVPMSGESR
                     LFITPGFRFKVVDALMTNFHLPRTTLLMLVSAFASIERMRRAYEEAVKERYRFYSYGD
                     AMLII"
     gene            complement(4665..>5105)
                     /locus_tag="VN039_00240"
     CDS             complement(4665..>5105)
                     /locus_tag="VN039_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HWV44450.1"
                     /translation="GQLVDSRIWIIAAEEMGVRVSDTDLRELIMQTPAFQKNGAFDPE
                     LYRRILAANHLTPASFESIQHQEVLANKARMIVRDSVALTPAEIAEGQSLMTRPQDSD
                     STKAAEAKQRVLDDILLQKQQRALISFQQSLKAKLPITIRRELL"
BASE COUNT         1089 a         1308 c         1532 g         1176 t
ORIGIN      
        1 tcatccgcaa agtcgatcgt gtgaccgggg tcatctcaac ggtagccggt atgtccgtcg
       61 aagacaacat gtgttctgct gtggaaggga cagcatctgc tcaagccacg gatgaagacc
      121 ctttggccga tggtgttgac aaaaccggtc aggcaaaatt tactcagcaa gccgatttga
      181 gcggaactgt tcggtattgg gcgaatcaat cagtcacaac taccaagcgg tatggcgggg
      241 acggcaagac cgcggtgcat gcgcagctga acttccctac ggcagtctcc gtggaccgag
      301 aggggaattt atatatcgcc gataccatga accatcgtgt gcggatggtc gattcagaaa
      361 caggggtcat ctcgactcta gccggaactg gtcaggctcg attctccggt gatggaggcc
      421 cggccaacaa ggctgcactc aatgaaccgg cagcgctggc cgttgacgag gcgggtcgac
      481 tctatatcgc cgatcaaagc aatcatcgtg tacgggtggt agacctcaag gccggtgtga
      541 ttcggaccgt tgctggaact gggattgcgg cgtacgatgg agatggaaag cctgctgttg
      601 acacggccct tgccggtcct agtggattgg ctctggcagg taatcatttg tatattgctg
      661 atactttcaa cggccgagtt cgatgcgtcg acctttcatc agggttgatg actatggtgg
      721 ccggcgatgg tggagtctat cggtatgagt ccccgtcgga tgccccctct ccaagccttt
      781 cacgcccgac aggaattgca ttggaccagg cggggcgtct ttttctgact gattcagaca
      841 atcacctcat tcggcagtgg gattggtcat ccggaacggc gttctgcgtg gcggggcaag
      901 gacttccatc ctattccggc gatggcggcg tcgcacgaga ggcaagtttg tgttacccat
      961 ttggtatcgt tgccgatcgc gacggaatgc tgctggtggc cgacaccttc aatcatcgca
     1021 ttcgcgtatt ggcattggag tagacgcttc atgatcacaa agaaaccagg gtacacggtg
     1081 gcgatccttg gcgcgaccgg cgcggtcggc aaggaaaccc tggagatctt ggaggagcgg
     1141 aaatttccgc tgacaagcct caggctcttt gcttccaaac gatcggccgg tgaagtgatg
     1201 acgtgccaag ggaaggagtg gaaggttgaa gagttgatgg aatcctcgtc gttcgagggt
     1261 gtcgatctgg cgtttatctc tgcgacggat gccatcagta aagactatgg ccaacgactg
     1321 ggagcggccg gtatcgtggt gatcgacgat agtgccgtgt tccgcatgga cgatcaggtt
     1381 cccttggtgg tgccggaggt gaacgctgcc gccttagggg ccatgcctcg gggaatcgtt
     1441 tccattccca actgtacgac cacgccgttg gtcatggtgc tcaagccgct tcacgatgcg
     1501 gtgggggtaa agcgtgtggt agtgacgacc tttcaatctg tgtcggggac cggtgcggcg
     1561 gccatggatg agttgatgga tcaaacgaag gatttgctgg cgtttcgcga tgtcacgacc
     1621 aaggtctatc cctaccagat tgccttcaat ctgttgcccc acatcggctc cttcaacgag
     1681 ggaggcgact gttcggaaga agtgaagatc gcgaaggaaa ctcgaaagat tctcgatgcg
     1741 ccgagtctcc gggttacagc caccacggtt cgcgttcctg tgctgcgttg ccattcagaa
     1801 gcgatcaatg ttgaattgga acgtccactg aacgcgaacg aagcgcgtgc cgcgctggca
     1861 gctatgcccg gcgtgattgt ctacgacgat ccggtgaaga agctgtaccc gatgccgctc
     1921 gatgtgaccg gaaaagatga ggtctacatc ggccgtgtgc gggaggatga atcaattgcc
     1981 aacggcctca atctttgggt ggtctccgat aatcttcgca aaggagcggc gctgaatgcc
     2041 attcagatcg cagaatgtct agtgaacgtc tagtagatgt gctgtctcac gggattgctt
     2101 gggcacgtca cccataggac gaaagtgata ccggaatgga caacgttcgg caaggtcctg
     2161 atcggaatag gcatcggtat tgctgcgctc gggtttctat tggtcgtcgt ggaccggatt
     2221 ccagggttag ggaacgcctt cacttggctc ggcaagcttc cgggtgacat ctcaatcaaa
     2281 cgtgataact tcagcttcta tttccccatc gcgaccagta ttgtcctcag cgtccttctg
     2341 agtctgctat tctatcttgt aggatggttc tttcgccgat gatagcagca gggcttttgg
     2401 ctggaactgc cggactgggc ctctccctcg gctcggtcat gattccggag gtccatgctg
     2461 cgcaatcgat tcgcgtgctg ttggcgccgg atgtccagca gttggaagtg catactgatc
     2521 agaccatttg gatgactgac gaacatgatc aggcgtcgtc ctatcggtct gtcctgcgga
     2581 tcaaggtgcg tggccatgcg ttgatcctca atggcaaacc ggtggtgagt gaacgactga
     2641 cgttacgggc cggaaatcac gatctcaaac tccggctgaa ccagaatggt aacaggactg
     2701 cgctccatgc gggtgatgag aaaggcgtac tccaagtcag tgggttgatc cagcttgttc
     2761 ggcgaggaaa gggactgctc ctcatcaatc acgtcgattt ggaagagtat gtcaagggtg
     2821 tggtgcctgc ggaagtgaat cccgcctggc accttgagat gctgaaggtt caggcagtcg
     2881 ctactcgaac ctatgcgttg tatcagcaca tgctcagctc cactcgggac tatgatgtgg
     2941 tgtcggggat tcaggatcag gtgtatcgcg gccggcaagg catcgacgcg cgggtggtgg
     3001 aggcggtgga atccacgagg ggactggttg taacctatca aggcgctccg atctatgccg
     3061 cgttctcttc gaccgccgcc gggattaccg aggatgccat gattgtatgg tcgaaagatc
     3121 tcccgtatct gaagggcgtg gaatgcccgt tcgacattga atctccgcac tatcagtgga
     3181 aggcttcctt gaagatcgac accttggaga aaaatttgcg ccaacaagga tttacggtgg
     3241 gaacaatttc ggcaatcatg ccgcttgcct atagccgcgc cggacgagtg gcaacgctgc
     3301 ggattgtgca ctcgagcggg gagttaatac tacgaggcga agatctgcgt aaagcggtgg
     3361 ggtatacggt ggtacccagt acgcaatttg ccgttgagtc ggtcggccag gacgtcgtca
     3421 tctccggcta tggcgccggc catgcggtcg gtctttgcca gtggggtgcg aaagagttgg
     3481 cggagctggg ttattccttt tcgagcattc ttcgctacta ctatccggga acggaacttc
     3541 aggatgcgtc gttgacccag gcgccttcag tgccttcccc cctctcggtc cctgcctcct
     3601 gatgcagctg tccgactttg actttccgtt cgattctctc cttattgcgt cccagccgat
     3661 acttcctcgg gatcgtgcgc ggctgctgat cgtggatcgt gcgacgcgac aacttgtcca
     3721 tcgtcaggtc gtagatcttc ccgaactact tcaatcaggc gaccttctcg tcgtgaatga
     3781 caccagggtc cgggctgcgc gggttcccgg aatcaaaaga cccacaggaa caccggtcga
     3841 agtgttgttc gtgaaggatc tcggtgacag ccgatgggag atcatggtca agggctcatt
     3901 tcgggtcggt caggtcatcg agtttgatca gcggtcatgc gcgaccatcg tgaagcgcga
     3961 tgcgataggc actgaagtga cgatagaaag tcctgcgccg gtcacacagt tatttgaagc
     4021 acgaggcatc atgccgctgc ccccgtacat caaacgcaca ccgactcagg aggatcatca
     4081 gtggtatcaa acggtctttg ccaaacatga gggcgcgatc gccgcaccga ctgccgggct
     4141 tcattttact gaaaaacttt ttcaacgact acgtgaagca gcgatcaagg ttgccacggt
     4201 gaccctccat gtcggccccg gcacgttcaa gccggtgacc accgaacgga ttgaagatca
     4261 tcagatggga acagaaagat tcacaatcag cgaagatgct gcaaaagcga tccgggagac
     4321 gaaacgagct ggtggtcggg tggtggccgt cggtacaacg gttgttcgca cgcttgaaac
     4381 ggttgcgatg gagaagggag agatcgttcc gatgtccggc gaaagcaggc tatttatcac
     4441 gcctggattt cggttcaagg tcgtcgatgc cctcatgacg aacttccacc taccgcggac
     4501 cacgctgctc atgttggtat ccgccttcgc cagcatcgaa cggatgcgaa gagcctatga
     4561 agaagcagtc aaagagcgat accgcttcta cagctatggc gacgcgatgc tgatcatcta
     4621 gctgcctgct gatccaggat ggctcaacaa gacgaggttt gactttacag caattctctg
     4681 cggatggtga tcggcagctt ggctttcagg gactgttgaa aggagattaa ggcacgttgc
     4741 tgcttctgca acaatatgtc atccaatacc cgctgcttgg cttctgccgc cttggtagag
     4801 tctgaatctt gtggtctagt catgagagac tgtccttcgg caatctccgc cggggtcagg
     4861 gcaacggagt ctcgtacgat catccgagcc ttattagcca ggacttcctg gtgctggatc
     4921 gattcaaacg acgcgggggt gagatgattg gccgctagaa tgcggcgata gagctctgga
     4981 tcgaaagctc cgttcttctg aaaagccgga gtttgcatga tcagttcgcg caagtcggta
     5041 tcggaaaccc gcacgcccat ttcttcagcc gcgatgatcc agatgcggct atcgaccagt
     5101 tgccc
//
LOCUS       DATMTI010000015         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_806755, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000015 DATMTI010000000
VERSION     DATMTI010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806755"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..761
                     /locus_tag="VN039_00245"
     CDS             138..761
                     /locus_tag="VN039_00245"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HWV44451.1"
                     /translation="MMTAADKGLMVCRTCGALCRRVRSSTQTQCPRCFDAVQFRKRDS
                     IGRTWAFLIAALILYIPANLLPVTETRTVFGSQQDTIMSGVVLFWTTGSPFIAAVVFA
                     ASVMIPMLKCIALALLTITAQRRSRWRPQQRTKLYRLIERIGRWSMLDVFVVTTVVAL
                     VHIRSLATIHAGPGAAAFGAVVVFTMLASKSFDPRLIWDPVETSHDS"
     gene            751..>1819
                     /locus_tag="VN039_00250"
     CDS             751..>1819
                     /locus_tag="VN039_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWV44452.1"
                     /translation="MTPENHDKLSDIPDPIVVKTTRWAPSLVWAVPILAALIGLSLIV
                     TTVLERGPTITIRFQTAEGLEAGKTKVKYKDVDIGEVKAITLSKDLGHVLIHLELVRG
                     TERFAVSDTRFWIVKPRVAASGVSGLGTLFSGAYIGTDPGQSSEKQKEFIGLEVPPIV
                     TTDVVGRQFTLHADDGGSLDIASPVYYRHVQVGQVVAFDLDQDGRGVTLKIFVQTPYD
                     RFVTPETCFWHASGMDVRMDANGVKVRTSSLAALLLGGIAFDAPPDIPTTAPAEEGRV
                     YVLAVDQEQAMKRQNEKAELAVLYFDQSVRGLSPGAPVDFRGLQLGDVKSIDVEFDPE
                     TREFRMPVTIEIYLERLGERYR"
BASE COUNT          338 a          508 c          564 g          409 t
ORIGIN      
        1 ctgtcgagta cagccagtat cctgcccggc gtggccttgt gggcttttgc ggcgctcacc
       61 gtgttgcttg ccgctattct gatgtttgag ccgcgccacc tttgggatct cgcagaagag
      121 ctcgcaggga gtcatgcatg atgaccgcgg ccgacaaggg gctcatggtg tgtcgcacat
      181 gtggggcact gtgccggcgc gtccgatcca gcacccagac gcaatgcccg cgctgttttg
      241 acgcggtcca gttccgtaaa cgggacagta tcggcagaac ctgggctttc ctcattgcag
      301 ccttgatcct ctatatcccg gcaaacttgc tgccggtgac cgagactcgt acagtattcg
      361 gttcgcagca ggataccatt atgagcgggg tcgtgctgtt ctggaccact gggtcgccgt
      421 tcatcgccgc agtcgtcttt gccgcgagtg tgatgatccc gatgttgaaa tgtatcgcgc
      481 ttgccctctt gaccatcacg gcacagcggc gttcgcgctg gcgtcctcag cagcggacga
      541 aactctaccg tcttattgaa cggatcggcc gctggtcgat gctcgatgtc tttgtggtga
      601 ccactgtggt cgcgctcgtc catatccggt cgcttgcgac gatccatgcc ggccccggcg
      661 ccgcggcatt cggagcggtc gtagtcttca cgatgctggc atcaaaaagt tttgatcccc
      721 gactgatctg ggatcctgtc gagaccagcc atgactcctg aaaaccacga taagctgtcc
      781 gacattcccg atccgatcgt tgtgaagaca actcggtggg ctccctcatt ggtatgggcg
      841 gttccgattc tcgccgcgct gatcggtctg tcgctcatcg tcaccacggt gttggaacgg
      901 ggaccgacta tcacgattcg cttccagacg gcggaaggtc tcgaggctgg aaaaaccaag
      961 gtgaaataca aggacgtgga tatcggcgag gtcaaggcga tcaccttgag caaggatctg
     1021 ggacatgtcc tgatccatct ggagttggtg agaggcacgg agcgattcgc cgtttcggac
     1081 acccgctttt ggatcgtcaa gcccagggtg gctgcgagtg gtgtgtcagg gctgggcacg
     1141 ctcttctcag gcgcctatat cggcaccgat ccggggcaat cgtctgaaaa gcagaaagag
     1201 tttatcggac ttgaggtgcc cccgattgtg acgacggatg tggtcggacg ccagttcacc
     1261 ctccacgcgg atgatggagg atcccttgac atcgcctccc ccgtctacta tcgccatgtc
     1321 caggtggggc aagtggtcgc gtttgatctg gatcaagacg ggagaggcgt gaccctgaag
     1381 atttttgtgc agactcccta tgaccgtttt gttacgcccg agacgtgctt ctggcatgcc
     1441 agtggtatgg atgtcaggat ggatgcgaac ggcgtcaaag tgaggacctc gtcgctggcc
     1501 gcgctgctgc ttggcgggat cgcttttgac gcgccgccgg acatcccgac aaccgctcca
     1561 gcggaggaag gcagggtcta cgttctggct gtagaccaag agcaggccat gaaacggcag
     1621 aacgagaagg cggagttggc cgtcctgtat tttgaccagt ccgtccgggg actgagtccg
     1681 ggtgcgccgg tcgattttcg tggcctgcag ctcggcgacg taaaatcgat cgacgtcgag
     1741 ttcgacccgg agacaagaga attccggatg ccggtcacca ttgaaatcta ccttgagcgt
     1801 cttggcgagc ggtatcgtg
//
LOCUS       DATMTI010000016         4200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_297693, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000016 DATMTI010000000
VERSION     DATMTI010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297693"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VN039_00255"
     CDS             complement(<1..1468)
                     /locus_tag="VN039_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV44453.1"
                     /translation="MQFVQNIGMPITDQNGRVVNLVGTLQNITGRKAARKALEESEAR
                     LALAQEAAGVGIWDWDIQAGTVSWSEKLYEMFGLEPGNLTPSFENWIECVHPDDRDRI
                     RLSTEQALDEGREIEEEYRIIRPDGHLRWLAGKGRTYKGEDGHPIRMIGIALDITERK
                     RTEEALRSTETRLQTTLSNLLEGYQIIGFDWRYLYVNEAAARHGRRRVSDLIGRRMTE
                     AYPRIEEQTPLFNTLRRCMDERIPATLTTEFTFADDSTGVFDLRIQPVPEGLFILSID
                     ITDRRQLELALRESEARYRAIVEEQTELICRFRPDTVLTFVNPAYYRFFEKTSEELLG
                     QTALWILPESQREGARVHIASLQANPRVVGCEHEVITSKGERRWLEWTDQAIMDEHGC
                     VIEIQSIGRDITERKQAEMTLRQLSGRLLQLQDEERRRIARELHDSTAQSLAALSMNL
                     TVVETESEALSDETRKALGESLALTKHSTRELRTLSYLL"
     gene            complement(1964..3997)
                     /locus_tag="VN039_00260"
     CDS             complement(1964..3997)
                     /locus_tag="VN039_00260"
                     /inference="COORDINATES: protein motif:HMM:NF028511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HWV44454.1"
                     /translation="MRMLADIGLWSWGNRHSVRHRLLTIALLPMLVIPPLLLGISIYR
                     WNAKFDAALVSKVNDDLTIAHQYLARILEESEEHLVSVTNSARFQEALRGHDYANGAL
                     TDLLRETAQRRGFDFLYIVGDDRHIIASEYPMVSSSMRWNWPILNSALDGRSKTGIDV
                     FEQAELSAVSPELAQRARIEIVSTVGSAPTTRTEEARGLVLQSASTLTLPDGRRAALV
                     GGILLNQNLGFVDTINDLIYHDSGLPEGSQGTVTLFLDDVRISTNVRLFDTRRAIGTR
                     VSAEVRSAVLDQGRTWLDSAFVVNDRYISGYEPILDSYNRRIGMLYAGFLERPFTEAK
                     RRTLLEIGLAFLLAVAATVPLFLRWAAEIFRPLERMIGTITQVESGDLDARTGHVESK
                     DEIGRVALHLDHLLDQIQERDAQLRQWNEELNQRVEGRTLELQQANRRLETTTQQLIM
                     SEKLAAIGEITAGVAHEVNNPIAVMQGNLEVLRDLMGSKAEAAKTEFRLIDEQLYRIG
                     EMVTRLLRFAKPQEYAGYSEQYDVAEIVTDTLPLVQHLFKKTTIAVERDHRASRLIQM
                     NRTELQQVLVNLLVNAIHAMPGGGRLTVRTFDRDEGDRPGVVIEVADTGAGMAPDVMR
                     KIFDSFFTTKGREGTGLGLSISRMLVTRQGGKVSVESEPGKGTTFAVWLPESH"
     gene            complement(3976..>4200)
                     /locus_tag="VN039_00265"
     CDS             complement(3976..>4200)
                     /locus_tag="VN039_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV44455.1"
                     /translation="LRNLIERTVILGAFPEDFERFVHDDDTGGQSLAEVEQRHILSVL
                     RETGGDREEAARRLGISRKTIDRKCACWRI"
BASE COUNT          857 a         1233 c         1147 g          963 t
ORIGIN      
        1 gtaacaggta cgagagggtc cttagttccc gagtcgagtg tttggtgagg gcaaggctct
       61 caccgagtgc ctttcgtgtt tcatcgctca acgcttccga ctctgtttca accactgtca
      121 gattcatcga taaggcggcc aagctttgag ccgtactgtc atggagttcg cgggcgatcc
      181 gtcgccgttc ttcgtcttgc aattggagga ggcgccccga aagctgacgt aaggtcatct
      241 cagcctgttt ccgctcggtg atgtcccgac cgatcgactg gatttcaatg acacaaccgt
      301 gctcgtccat aattgcctga tcggtccatt ccagccaccg ccgttcacct ttggaagtaa
      361 tgacctcatg ttcgcagccc accacgcgcg gattggcctg caacgaagca atgtggaccc
      421 tggccccttc acgttgactt tccggaagta tccataatgc ggtctgacca agcagttctt
      481 ccgaagtctt ctcaaaaaat cgatagtagg ccgggttcac gaaggtgagg accgtatcag
      541 ggcggaaccg gcagatcaac tcggtctgct cttcgacgat ggcccggtac cttgcctcac
      601 tctctcgcag ggccaactcc aattgcctgc ggtcggtgat gtcaatggac agaatgaaga
      661 gaccttcagg aacaggctga atgcggagat cgaagactcc cgttgagtca tcggcaaagg
      721 taaactccgt tgtcagcgtg gcagggatgc gctcgtccat gcaacgccga agtgtgttga
      781 acagcggtgt ctgttcctcg atgcgtggat aagcctccgt cattctccgt ccgattaaat
      841 cactgaccct gcggcggccg tggcgcgccg ccgcctcgtt cacatacagg tacctccaat
      901 caaacccgat gatctggtac ccctccagca gattgctcag ggtggtctga agccgggtct
      961 cggtactccg tagcgcctct tcggttcgct tgcgctcggt aatgtcgagc gcgatgccga
     1021 tcattcggat cggatggccg tcttcaccct tataggtccg tcctttcccc gccaaccaac
     1081 gaaggtgtcc atccggtcga atgatccgat attcttcctc gatttctcgc ccctcgtcca
     1141 acgcctgctc ggtggagaga cggattcggt cacgatcatc cggatggacg cattcaatcc
     1201 agttttcaaa tgacggtgtg agattccctg gttccagacc aaacatttcg tacagttttt
     1261 ccgaccaact cacggtgccg gcctggatat cccaatccca aatccccact ccggcggctt
     1321 cctgggccag ggcaagccgc gcttcactct cttcgagggc tttgcgtgcc gctttacgtc
     1381 cggtgatgtt ctgcaaagtg ccgacaaggt tcacgactct cccattctga tccgtgatcg
     1441 gcatcccaat gttctgaacg aattgcatcg agccgtcatg gagcgaccga tttacatttc
     1501 ggtcaagcag ccgccctttc tacgagcgaa gcagtggcat gttcggttca attttgctgt
     1561 catggtgtaa cctgagtttt tcctatgatg gtctgcacca aaaagtttgg acaacatcca
     1621 atcgtttgtc agtccgctcc atacttccac atgaggtcag aagcaacttg cttcaggagc
     1681 aatatgagaa acgttcgtga gatcacagtc tctcattttg gaacgctcgg tatccgtttt
     1741 ttgattttcg agatcgtgtg ggttgtcact tccgcgtgtc atggtgcata gactgtcggc
     1801 ctgtcgtggt ctatggaggg caatgacaag ttggtgacaa gggcatcgtc gccgtccgca
     1861 gactataaaa actcctcaaa aggcgataac tcgggaaaca cctagtattt ttgccgatga
     1921 ggttcgaccg tttcaaaccg tcgtggcggg aagagaggcg acatcaatgc gattcgggca
     1981 accagacggc gaacgtcgtg cctttgcccg gctcactttc gaccgaaacc ttgccgcctt
     2041 gccgggtcac gagcatccgg ctgatggaga gaccgagccc cgtgccttca cgccccttgg
     2101 tcgtaaagaa cgagtcgaag attttccgca tgacgtcggg cgccatgccg gcaccggtat
     2161 ccgcaacttc gatgacgacc cccggacggt cgccttcatc acggtcgaac gtccgcacgg
     2221 tgagccgtcc gccaccgggc atcgcatgaa tggcattgac cagcaggttc acaaggacct
     2281 gctgtaactc agtcctgttc atttggatga gtcgcgacgc gcgatggtct cgctcgacgg
     2341 caatcgtcgt cttcttgaac agatgttgga ccagcggtaa cgtgtcggtg acgatctccg
     2401 cgacgtcgta ctgttcggag taacctgcgt attcttgcgg cttcgcaaat cgaagcagcc
     2461 gagtgaccat ttcaccgatg cggtacagct gctcgtctat aagccgaaac tccgtctttg
     2521 ccgcttcggc cttgctgccc atcaagtcgc ggagcacctc caaatttccc tgcatgaccg
     2581 cgatcggatt attgacctca tgcgccacgc cggcggtgat ttcgcctatc gcggcaagtt
     2641 tttccgacat aatgagttgt tgggtcgtcg tctccaatcg tcgattggct tgttgcagct
     2701 cgagcgtgcg tccctcgaca cgctgattga gctcctcatt ccactggcgc agctgcgcgt
     2761 cgcgctcctg gatttgatcg agcagatgat cgagatgaag cgccactcgg ccgatctcat
     2821 ccttactctc gacatggccg gtccgtgcat caagatcgcc gctctcgacc tgggtgatcg
     2881 ttccgatcat ccgctcaagc ggtctgaaga tttcggcggc ccatctcaga aatagtggaa
     2941 cggtcgcggc gactgcgagc aggaacgcaa gccctatctc cagcaaagtc cgacgcttcg
     3001 cttcggtgaa gggcctctcg agaaaccccg catagagcat gccgatgcgg cgattgtagc
     3061 tgtcgaggat cggctcatac cccgagatgt accggtcatt gacgacaaag gcgctatcga
     3121 gccacgttcg accctggtcg agaacggcgc tccgcacttc cgccgagacg cgcgttccaa
     3181 tcgcccgtcg agtgtcgaac aagcgcacat tggtgctgat gcgaacatca tcgaggaaca
     3241 gcgtgactgt cccctggctg ccctcgggca gaccggagtc gtgatagatg aggtcattga
     3301 tcgtgtcgac gaaaccaagg ttttgattca acaggatgcc gccgaccaag gcggcgcgcc
     3361 ggccgtcagg caacgtcagc gtgctcgcgg actggaggac cagaccgcgc gcttcctctg
     3421 tgcgcgtggt gggggcgctg ccgacggtcg aaacgatttc aattcgcgcc cgttgagcga
     3481 gttcaggcga taccgccgaa agctccgcct gctcgaacac gtcgatgcct gtctttgagc
     3541 gaccgtcaag agccgagttg aggatgggcc agttccagcg catggaactc gaaaccatcg
     3601 gatattcgct tgcgatgata tgtcggtcat cgccgacaat gtagaggaaa tcaaacccac
     3661 gtctctgcgc ggtctccctg agaagatctg taagggcgcc gtttgcataa tcgtgtcctc
     3721 ggagcgcctc ctgaaatcgt gcggagttcg ttacggaaac caggtgttcc tctgactctt
     3781 ctagaatgcg cgcgagatat tggtgagcga tggtcaaatc atcgttcacc ttggagacca
     3841 gcgctgcatc aaacttggcg ttccaacgat aaatgctgat cccgagcagc agaggtggaa
     3901 taacgagcat cggcagaagt gcgatcgtga gcagacggtg gcggaccgaa tgccggtttc
     3961 cccaagacca cagtcctata tccgccaaca tgcgcatttg cggtcgatcg tcttgcgtga
     4021 aatgccaagc cgacgcgcgg cttcttcccg atcgcccccc gtctcgcgga gcacggacag
     4081 aatgtgccgt tgttccactt cggcgagact ctgcccccct gtatcgtcgt catgcacgaa
     4141 tctctcgaag tcctccggaa aggccccaag aatcaccgtt cgctcgatga ggtttcgcaa
//
LOCUS       DATMTI010000017         6446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_594737, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000017 DATMTI010000000
VERSION     DATMTI010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594737"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VN039_00270"
     CDS             <1..1353
                     /locus_tag="VN039_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWV44456.1"
                     /translation="LADLHMLISETPQGLYPYAGVPWFSVPFGRDGIITALQMLWMNP
                     ALARGVLGFLASTQATENRPEQDAEVGKILHETRQGEMAALGEIPFGRYYGSVDATPL
                     FIVLAGAYYERSGDRRFLTALWPHIQLALQWIDQYGDPTGMGFTTYARRTDKGLVHQG
                     WKDSHDAVFHADGAAAEGPIALCEVQAYVYQAKRTASDLARLLGNPDHADRLLKEADS
                     LRDRFERAFWCEELDSYALALDGRGRPCRVRSSNAGHCLYGGIAGKERGVRTAHALLS
                     KELFSGWGIRTIATSEARYNPMSYHNGSVWPHDNAIIAAGMAAYGHKQGAMKVLTGIF
                     DTSLFLELHRLPELFCGFSRRAGESPTLYPTACSPQAWASGAGFLLLQACLGLRIDAP
                     QRLVSFVRPALPPFLERVEIRNLSVGTGRLDLVLDRHENEVALRVIRRIGEVDVVFTI
                     "
     gene            complement(1355..1621)
                     /locus_tag="VN039_00275"
     CDS             complement(1355..1621)
                     /locus_tag="VN039_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44457.1"
                     /translation="MGNSGARSPASREPEQVILLHVVDKPALQALVGKNPVGERRVLA
                     AMEKAGGILLRDAARSARVALGQAATAPRTNIRTIVAHGCFLRI"
     gene            1905..2360
                     /locus_tag="VN039_00280"
     CDS             1905..2360
                     /locus_tag="VN039_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV44458.1"
                     /translation="MNKTKRTKKRAKVVKVKDFDSMTVSQVMEKEVQCIRLRTKGDMI
                     ASLMIEGFGAVPVVENGRKLAGIVSEHDLLVAIDDRHHLNAVAAKDIMTSNPYSVRAE
                     TTLGTLVHVLRASDLVRVPVVDAKDKLIGIVARRDVLRTYLATGGKREQ"
     gene            2368..2802
                     /locus_tag="VN039_00285"
     CDS             2368..2802
                     /locus_tag="VN039_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV44459.1"
                     /translation="MRQTDFFMKASDPKALTVEQLMQDAVTRCTSRTDASTIAHIMTH
                     RNFGSLPVVEEDGTLIGIVTEYDLLQAMLDGRDLRKILATEIMSAHPVTVTEDQTLAQ
                     VADLFQDRYLTRVPVVRNNKLVGILARRDLLYGYMKASQYWS"
     gene            3312..3464
                     /locus_tag="VN039_00290"
     CDS             3312..3464
                     /locus_tag="VN039_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44460.1"
                     /translation="MEMGKWFKMLRNRDHGIHDVAADELFNFAVMVTPVQEYGIMVPT
                     QTPAIS"
     gene            3528..4034
                     /locus_tag="VN039_00295"
     CDS             3528..4034
                     /locus_tag="VN039_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44461.1"
                     /translation="MRLAIALMAGGVLLLCVAVPATADESGMVDASHYYWLTGVVARI
                     QSGMLFVQPPVGLQARAISPRRVERVGLHNAKIGDKVNLWVDSGNVLIDARADGLPAP
                     DHRVIAGRLTYSDASWSEIKISTPEGTQVFEVDSLAGSKFSTFAEGMPVEVELDADNF
                     VIDVYRAQ"
     gene            4183..4974
                     /locus_tag="VN039_00300"
     CDS             4183..4974
                     /locus_tag="VN039_00300"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HWV44462.1"
                     /translation="MTQMVLRWGMVVWLLGAATVFADTAPNRERESDRSPTFDALIEA
                     EKTARLLAVLLDSGRAVISANQPLFDGTSKGTKGFTPEAFERQLVEVFRSRSGLDLND
                     LASARLSTKAVRLLRSLVSVSKQVMAEVQGQTHSDGTPSGVIPAVFGARAAAQFTKRT
                     GVQLKQTALAPRNPVNAPDPFEQAALQDFADPAYPREKTITEVTARSGALHLMFPLYA
                     TRHCLDCHGEPKGEIDKTGYPKEGLQLGHNAGAISVVIPVPVVKK"
     gene            4971..>6446
                     /locus_tag="VN039_00305"
     CDS             4971..>6446
                     /locus_tag="VN039_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWV44463.1"
                     /translation="MTSVRLLVILGGLLLLPVGIGRGAEPALVPPERPSGTGTIIHSE
                     GYILTAYHVVAGARRIVVVTSGEVRAPALIVSSDQEHDLALLKIDAVGLSEAALGYAG
                     SVRLDQEVITLGFPFGLRDISVTRGRIAAMRTKGVRRVFQVDAAINPGNSGGPVFNRR
                     GEIIGILTTKFSHPSGIIPEGMSFAVPLSYATPLLANIPEFDFSTIGKGRRETKAGDK
                     NGDLIRELARITVRIETVRGDSSAPTTAASASGQAKAEEAGALSRSPRVSTVPPAREP
                     APPAAKENEPSAPIQQTEMQRLKQRGVVGPEDMLLVPEGEFLMGADDGLPDARPVHRV
                     HLSAYWIDKYEVTNASYRRCVEAGVCQPPKNRGPFEDQTRDQYPVTSVTWMQARSYCQ
                     WAGQRLPTEAEWEKAARGIDGRRYAWGNSQDPILNRGKNGENQVNHGEPQPVGSFQEG
                     ASPYGVLDMTGNVWEWVKDWYAEDFYAVAPPHDPQGPPRGSF"
BASE COUNT         1295 a         1793 c         2058 g         1300 t
ORIGIN      
        1 ctcgccgatc ttcacatgtt gatctcggag acgccgcaag gactctatcc ctatgccggt
       61 gtgccctggt tcagcgtccc gttcggacgg gacggtatta ttacggcgtt gcagatgctg
      121 tggatgaatc ctgctctcgc gcgaggcgtt cttggctttc tggcctcgac tcaggcgacg
      181 gagaatcggc cggaacagga tgcagaggtc gggaagattt tgcatgagac gcgtcaaggc
      241 gagatggcgg ccctcgggga aattccattc ggtagatact atgggagtgt cgacgccacg
      301 ccgttgttca ttgttctggc gggcgcgtat tacgagcgga gcggcgatcg ccggtttctc
      361 acggcgctct ggccgcatat tcagttggcc ctgcagtgga tcgatcagta cggcgatccg
      421 accggtatgg gtttcacgac ctatgctcgg cggaccgaca agggactggt gcaccaagga
      481 tggaaagact cccacgatgc cgttttccac gccgacgggg cggctgccga agggccgatt
      541 gcgctctgtg aggtccaggc ttatgtgtat caagccaaac gaacagcgtc ggatttggcc
      601 cggcttttgg ggaaccccga tcacgccgat cgattgctga aagaagcgga ctcgcttcga
      661 gatcgattcg aacgggcgtt ctggtgtgag gagctggact cgtatgccct tgctttggac
      721 ggacgggggc ggccttgccg tgtacggtca tcaaacgccg gtcattgcct ctatggcggc
      781 attgccggga aagaacgtgg agtacgcacg gcacacgcac tgttgagcaa ggagcttttc
      841 agtggatggg gcatccgcac gatcgcgacc tcggaggcac ggtacaaccc gatgtcttac
      901 cataacgggt cggtgtggcc acacgacaac gccatcatcg cggccggcat ggcggcgtac
      961 ggccataagc aaggagcgat gaaggtcttg acgggcatct ttgataccag cctctttctc
     1021 gaactccatc ggctgccgga attgttctgc ggattttctc gacgtgccgg ggaaagccct
     1081 acgctctatc cgacggcctg ttctccgcaa gcttgggcgt ccggtgccgg cttcctgctg
     1141 cttcaagcct gtctgggctt acgaatcgat gcaccgcagc gtctggtgag tttcgtccgt
     1201 cctgcgttgc cgccgttcct ggagcgagtc gagattcgga acctctccgt cggtaccggg
     1261 cgtctcgacc tcgtgcttga tcgccatgag aacgaagtcg ccttgagagt gatccgccgc
     1321 atcggcgagg tggacgtcgt gttcaccata taaatcatat ccgaagaaaa cagccatggg
     1381 ccacgatggt ccggatgttt gtgcgcggtg ccgtggcggc ctgaccgagg gccacgcggg
     1441 ccgatcgcgc cgcatcccga aggagaatac cgccggcctt ttccatggcc gccagaacgc
     1501 gacgctcgcc gacgggattc tttccgacga gtgcttgcaa ggccggcttg tcaacgacat
     1561 ggagcaagat gacctgttca ggttctcggc tcgcagggct tcgagcgcct gaattcccca
     1621 ttgtgaatgt tccgacccat cgactgcaca taagattcgc atgataggct cctccgcagt
     1681 ggctccgcct cgagagaaag gtttcgggta tcagttctgc atcggatgtg tctgtggcat
     1741 ggagagtcat ggaagacaaa ttggtcatct ttcgaagagt tatgcacgtt ctctgtgttg
     1801 caatccgatg ggaggttgcg gcgttttgcg gcatgagata ggtgacttgc tgtggtgatt
     1861 agcgccagtg ggcagcgaag taagccgatc ggtaaggaga ggagatgaac aaaacaaaga
     1921 ggactaaaaa gagggccaag gtggtcaagg tcaaagactt tgattccatg acggtcagcc
     1981 aggtgatgga gaaagaagtg caatgtatcc gcctgaggac gaagggagac atgattgctt
     2041 cattgatgat cgagggattc ggggctgtcc cggtcgtgga gaatgggagg aaactggccg
     2101 gcatcgtcag cgagcacgat ttactcgtgg ctattgacga ccgtcatcac ctcaatgccg
     2161 tcgcggcaaa ggacatcatg acttccaatc cttattcggt aagggctgaa acgacgctcg
     2221 gaacgttggt ccatgtgctg cgcgcaagcg atctcgttcg tgttcccgtc gtggatgcca
     2281 aggataaact gatcggtatt gtcgcgagac gcgatgtctt gcgaacctat cttgccaccg
     2341 ggggaaaacg agaacagtga ggagcttatg agacaaaccg acttcttcat gaaagccagc
     2401 gaccccaagg ccttgaccgt cgagcaattg atgcaggacg cggttaccag atgcacgtcg
     2461 cgcaccgatg cctcgacaat tgcacacata atgacgcatc gcaacttcgg aagtcttccg
     2521 gttgtggagg aggatggcac gttgatcggg atcgtaacag aatacgatct cctgcaggcc
     2581 atgctcgacg ggcgcgatct ccggaagatt cttgctacgg aaatcatgtc tgcgcatccc
     2641 gtgaccgtga cggaagatca aacacttgcc caagtggccg atctgttcca ggatcgctat
     2701 ttgactcgcg tgccggtggt ccggaacaac aagctggtgg gaattctggc tcggcgtgac
     2761 ctgttgtacg gctatatgaa agcgtcgcaa tattggtcgt gattcaaact ctcatcctac
     2821 ggcagaaggc ctggatgcgc gtaagcacaa agccgacatc acgcctcgca cgatcgatcc
     2881 tgaacaaatt cactacggcc tacctagggg gctcccatct ggttgtacag cccggcacca
     2941 ccgatatggg atttcgtcga gcacaaccgc taacagtttt atgtaccgat gcgtcgagac
     3001 ggatcgaagt atttccactt cacgatcttg gggcgctagt cacgcgtctc tccgccggcc
     3061 tcatccgata gagtgaggca aaatacaccc ccctgggtaa tcgctctttt ccctgtgatc
     3121 ccgatagagt atggctgttt tgatacgtcc tcggtttcca aagtgagggc aggggcaacc
     3181 ataccgatga gctgtctaag tcggtcagct tctataaggc gacctacccg aactccgatt
     3241 ggaaacccta cgaggacaaa cttacgctgg tgcggcaagc tgtggatcga ggggactagc
     3301 ggacggttcg gatggagatg ggcaagtggt tcaagatgct ccgcaatcgg gaccatggga
     3361 ttcacgacgt cgcggcagat gaacttttca atttcgcggt catggtcaca ccggtgcagg
     3421 aatacgggat tatggtgccg actcagacgc cggcgatttc gtaggcgacg ggagggacta
     3481 tggccgagat ttaggggcat gggcctctca gtaaggaggt gggcatcatg cgattggcta
     3541 tagcgttgat ggcaggcggc gttctgttgc tgtgtgtggc cgtcccggcg acggcggatg
     3601 aatcgggcat ggtggatgcc agtcactatt attggttgac gggggtcgtg gccaggatcc
     3661 agtccgggat gctgttcgtg caaccgcccg tcggcctgca agcgcgggcg atcagcccaa
     3721 ggcgggttga gcgggtcgga ttgcacaacg caaagatcgg cgacaaggtc aatctgtggg
     3781 ttgattcggg taacgtattg atcgatgcgc gcgcggatgg tttgccggcg ccggaccatc
     3841 gggtgatcgc gggcaggctt acctacagcg acgcatcctg gagcgaaatc aagatctcga
     3901 cgcccgaagg cacccaagtc tttgaagtgg acagtctggc ggggagcaaa ttttcgacat
     3961 ttgcggaggg catgccggtc gaggtcgagt tggatgccga caatttcgtc atcgacgtgt
     4021 atcgagccca gtagtgtcgg cgcggcttga cttgtctttc cggcgacgat taccatttgt
     4081 accggtctcg gatctccggc aagactccat tcggaccgga gatcttgaga cagtgatcat
     4141 ctgctttctc ccgtggactg atagggtggc tgggaggtgt cgatgacgca gatggtctta
     4201 cggtggggga tggtcgtgtg gctgctgggt gccgccacag tgtttgctga cactgctccg
     4261 aaccgcgagc gggagagcga caggagtccc accttcgacg cgctgatcga ggcggaaaaa
     4321 acagcgcggc tcctggccgt gctcctcgat tcggggcgcg cagtcatcag cgcgaaccag
     4381 cctctcttcg acggcaccag caaaggcaca aagggattca cgccggaagc cttcgagcgg
     4441 cagctcgtcg aggtctttcg gagccgttcg ggcctggacc tgaacgattt ggcctcggct
     4501 cgtttgtcga cgaaagcggt tcgccttctc cgatcgctgg tttccgtcag caaacaggtg
     4561 atggcagaag tgcagggcca gacccactcc gatggcacgc ccagcggggt cattccggcc
     4621 gtgtttggcg cgcgggcggc ggcacaattc acgaagcgga ccggagtcca gctgaagcag
     4681 accgcgttgg ctccccgaaa ccccgtcaac gcccccgatc catttgaaca ggctgcgttg
     4741 caggacttcg ccgatcccgc ctatccaaga gagaagacca tcaccgaagt tacggcgcgg
     4801 agcggtgcgc tgcacctgat gtttcctctc tatgcgacgc ggcattgtct ggactgccat
     4861 ggcgagccca aaggggaaat cgacaagacg ggctatccca aggaagggct ccagcttggc
     4921 cacaatgccg gggcgatcag cgtggtgatc ccggtcccgg tggtcaagaa atgacgtccg
     4981 tgcgtctgct cgtgatcctc ggaggtctgc tcctgctgcc ggtcggaatc ggcagggggg
     5041 cggagcctgc gcttgtgccg cccgaacgac cctctggaac cggaaccatc attcattccg
     5101 aggggtacat tctgacggcc taccatgtcg tcgccggcgc gcgacgcatc gtcgtggtca
     5161 cgtccggtga ggttcgcgcg ccggcgctga tcgtcagctc ggatcaggag cacgacctcg
     5221 ctttgctcaa gatcgacgcg gtggggctct cggaagcggc actgggctat gccggttcgg
     5281 tccggctgga tcaggaggtg atcacgctcg ggtttccgtt cgggctgcgg gatatctccg
     5341 tgacgcgggg ccgaatcgcg gcgatgcgga ccaaaggggt gcggcgcgtg tttcaagtcg
     5401 atgccgcgat caatccggga aacagcggag ggccggtatt caatcggcgc ggcgagatca
     5461 tcggcatcct aacgaccaaa ttcagtcatc cctcggggat catccccgag ggaatgtcgt
     5521 ttgccgtccc gctcagttac gcgacgccgc tcctcgccaa cattccggaa tttgattttt
     5581 ccaccatcgg caagggccgt cgagagacaa aagccggcga caagaacggc gacctcatcc
     5641 gtgagctggc gcgtatcacg gtccggattg aaaccgtgcg aggcgactcg tcggctccca
     5701 ccaccgcagc gtcggcttcc ggccaagcaa aagcagaaga ggcaggcgcc ctgtcccggt
     5761 cgccgcgggt ctcgactgtt cctcctgcgc gagagccggc ccctcccgcc gccaaggaga
     5821 acgagccctc cgcccccatc caacagaccg agatgcagcg gctgaagcag cgcggagtgg
     5881 tcggtcccga ggacatgctg ctggtgccgg agggagagtt cctgatgggc gcggacgatg
     5941 gattgccgga tgcgcggccc gtccatcgcg tgcacctgag cgcctattgg atcgacaagt
     6001 acgaagtgac caatgccagc taccgccgct gcgtcgaggc gggggtctgc cagccgccca
     6061 agaaccgcgg cccgttcgag gaccaaacca gggaccagta tccggtgacc agcgtcacct
     6121 ggatgcaggc ccgctcctat tgccagtggg ccgggcagcg attgccgacc gaagcggaat
     6181 gggagaaggc cgcgcgggga atcgatggga gacgctatgc ctggggcaac agtcaggacc
     6241 cgatcctgaa ccggggcaag aacggcgaga accaagtgaa ccatggcgag ccgcaaccgg
     6301 tcgggagttt tcaggaaggg gcttccccct atggagtcct ggacatgacc gggaacgtct
     6361 gggagtgggt caaggattgg tatgccgagg acttttatgc ggtcgcccca ccgcacgatc
     6421 cccagggacc gccgaggggt tccttc
//
LOCUS       DATMTI010000018         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_806864, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000018 DATMTI010000000
VERSION     DATMTI010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806864"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VN039_00310"
     CDS             <1..1496
                     /locus_tag="VN039_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HWV44464.1"
                     /translation="TDRTDRADASTSKTSERDRSDENMNKSAQASKKDVAREDGERST
                     VKDDARSDSNSSGSQSVSAPAPLPIATQTINQEQTSTDDGPRSSEDEHQATTASSMST
                     LIPSEAPMPSMTAPEAHKGASSSDETAAQSKDQPEQASAMSALSTKGDAQAGPIVKDD
                     VQAVVSGTDTKTVVAAVEPHAASSGEEADRSRPQSDAPARRESNLNSGQTPNNGGIDR
                     PMNDAVRMVEGVTLVRPVSPHSDGHVPSRGDKEEVKSKTEVVLPIEDPAQFFSDDGNG
                     ESKVSAALLRGQQSSFDVTKHFVELKAGHIGSPHDQAEIELPQAAVGEHQAAGGQPTG
                     AIMAGAQGGISSSSPPPPPTTPFVSHAPPAVPGHDPGDKSAQVMTRSVVFDVAQPDLG
                     HVNIRVAMTNDVVHTHLSADRPEVGQFLVNGQDRLQAAFQANGLDMGQFRVDIDRQSG
                     GRSFQHGPSQEQGQAWNQGSQGMNWGQSQDRQDEQHTSLHGLLNLVA"
     gene            1518..2171
                     /locus_tag="VN039_00315"
     CDS             1518..2171
                     /locus_tag="VN039_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HWV44465.1"
                     /translation="MVDVSQVNSTGTQSTPEKTGPQQLGQDDFLKLLITQLQNQDPLK
                     PTDNTEFVSQLAQFSQLEQTAKQADLLQKSLDAQTASLQYTLLPMVGRQVSIDRPLTQ
                     LENGQASLTYALEKNAARLQITILDQSRQVVRTLDYTNLQAGLNQTQWDGKDSKGVAM
                     PPGVYEYAISAVDQQGASVVATGHAQLTVTGIRMEEGQPKLAIGTLTVDPSEIVEVQ"
     gene            2214..3950
                     /locus_tag="VN039_00320"
     CDS             2214..3950
                     /locus_tag="VN039_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HWV44466.1"
                     /translation="MGILSSLFAGVSGLNANGTALSVIGNNIANLSTVGFKGSKATFA
                     DLISSSISGGSGAVQTGIGVALTSVQGNFSQGSLTTSSNVLDLAIDGNGFFIVKDAQG
                     GTFYSRAGQFRLDKDNNVVDPTGFKVQGFLADTAGTITGTIGDISLPSTTASPRATTT
                     ALVAANLNSATDPTGVRGNVVASAASVTTTAAGNNSFTINLNGDGARTITVANGLTGS
                     ALATAIQNAVRALVPNDPFKGAAYAGFTASVNASNTFTFASGMTGTTNNSTTGTGTVV
                     VAANGGDTLAANLNMLAPTSTTGTDFLLSDPSATSNFSTSMTVYDSLGNSHLLTTYFT
                     KVGENSWNYNTVAAATDVVTANYHSSNIDTSLGIVRVGSGTLTFGTDGTLDRESTVIR
                     YDTATAAGTSGTAPGELQLDFVGATQDQPIAMNFGSSVTTDGGSGLDGTTQFGSTSAL
                     VQQTQDGFAAGSLQAFSVDANGTINGRFSNGQVRALAQVVLARFPDPIGLTRTGKNTF
                     AQSGDSGQPVTGTPDSAGLGQVKSNSLELSNVDLGESFIDMIAAQRGFQANSRVITTS
                     DEILQELVNLKR"
BASE COUNT          969 a         1266 c         1296 g          864 t
ORIGIN      
        1 gaacggatcg tacggatcgt gccgacgcat cgacttcaaa gacgtcggag agagaccgtt
       61 ctgacgagaa catgaacaag tcggcccagg cttcaaagaa ggatgtggca agggaggatg
      121 gtgaacgttc cactgtaaag gacgatgccc gttccgattc taacagctca gggtctcagt
      181 ccgtatctgc tccggctcca cttccgatcg cgacacagac gatcaaccaa gaacagacct
      241 ccacggacga tggtcctcga tcgtccgaag acgagcacca ggcaacaaca gcgtcctcca
      301 tgtccacgct gataccgtcc gaagccccta tgccttcaat gacggctcct gaggctcaca
      361 aaggcgcttc gtcctcagat gaaacagctg cgcaatcaaa ggaccaacca gaacaggcat
      421 cggccatgtc ggcgctttct acgaagggtg atgcgcaagc tggtccgatc gtgaaagatg
      481 acgtgcaagc cgttgtcagt ggcactgata caaagaccgt ggttgctgct gtggaacctc
      541 atgcggcttc ttcaggggag gaggccgatc gatctcgtcc acagtccgac gctccagctc
      601 gaagagagtc caatctaaat tccggccaga cgccgaacaa tggagggatc gaccgtccga
      661 tgaatgatgc tgttcggatg gtggagggag tcacgcttgt acgtcccgtt tccccacatt
      721 cggatggcca tgtgccgtct cgtggcgaca aggaagaggt gaaatcgaag actgaagtcg
      781 tattgccgat tgaagatcct gcacaattct tcagcgacga tggaaacggt gaatcgaagg
      841 tcagtgcggc cttactgcgt ggccaacaat cgagttttga tgtgacgaaa cacttcgtcg
      901 agttgaaggc cggtcacatc ggctctccgc atgatcaagc tgagattgag ttgccgcagg
      961 cagcggttgg tgagcatcaa gcggcgggcg ggcaaccgac aggggcgatc atggccgggg
     1021 ctcaaggagg catatcgtcc agttcgcctc cgccgcctcc caccacccct ttcgtgagtc
     1081 atgcgccgcc tgccgtgccc ggccacgatc ccggagataa atccgcacag gtgatgacgc
     1141 gatcggtcgt gtttgatgtg gcacagcctg acctgggcca tgtgaatatt cgcgtagcga
     1201 tgacgaacga tgtggtccat acccatttgt cggccgaccg gcctgaagtg gggcaattcc
     1261 tcgtcaacgg acaggaccgt cttcaggcgg cgtttcaggc gaacggactg gacatgggac
     1321 aattccgcgt ggacatcgat cgtcagagtg gcggtcgttc cttccagcat ggcccttcgc
     1381 aggaacaagg acaggcctgg aatcagggtt cgcaagggat gaactggggg cagagtcagg
     1441 accgacagga cgaacaacac acgtcgctcc atggcttatt gaatttggtg gcgtagacaa
     1501 taaaggagat accgcaaatg gttgatgtat cacaagtcaa ttcgacggga acccaatcga
     1561 caccagaaaa aaccgggcct caacagttgg gacaggatga ttttctcaaa ttgctcatca
     1621 cgcaattaca gaatcaggat ccattgaaac ccaccgacaa taccgaattc gtctcgcagc
     1681 tcgctcaatt cagccagttg gagcagaccg cgaaacaggc ggacctgcta cagaagagcc
     1741 tcgatgcaca gaccgcctcg ctgcagtaca cgctgctgcc catggtcggc cgccaggtca
     1801 gtatcgatcg tcccctgacc caattggaga atggtcaggc ctcgctgacc tatgcactcg
     1861 aaaagaacgc ggcgcgcctg cagattacga ttctcgatca gagtcgccag gtcgtgcgga
     1921 cgttggacta caccaacctc caggcagggc tcaatcaaac acaatgggac gggaaggaca
     1981 gcaaaggcgt cgccatgccg cccggtgtgt atgagtatgc catctctgcc gtcgatcagc
     2041 agggtgcgtc ggttgttgca acaggccacg cgcaactcac cgtcacaggc atccgtatgg
     2101 aggaggggca acccaagttg gcgattggaa cgctgactgt ggatccgtcg gagatcgtgg
     2161 aagtgcaata gcagcggtga atctcaacgg gctgaagaag gaggcatgca cccatgggaa
     2221 ttctgtcgtc attgtttgcc ggagtcagtg ggcttaacgc caatggtacg gcgctctccg
     2281 tcatcggcaa caacatcgcc aacctcagca cggtcggctt caagggaagc aaggcgacgt
     2341 ttgcggactt gatcagttcg tcgatttcag gtgggtccgg tgcggttcag accggcatcg
     2401 gcgtggcgct cacgtccgtg caggggaatt tctctcaagg atcgctgacg acctcatcca
     2461 atgtgctgga ccttgccatc gacggcaacg gatttttcat cgtgaaagac gcgcagggcg
     2521 gtaccttcta ttcacgtgcc ggccagttcc gtctcgacaa ggacaacaac gtggtcgatc
     2581 cgacgggatt caaagtgcag ggattcctcg ccgacaccgc cggtaccatc accgggacga
     2641 tcggcgatat ctcactgccc tcgaccaccg catcgccgag ggccacgacc accgctctcg
     2701 tagccgccaa tttgaattcg gccactgatc cgacgggtgt tcgcgggaac gtcgtggctt
     2761 ccgctgcctc tgtgaccacc actgcggctg gtaacaattc gttcacgatc aacttgaacg
     2821 gtgatggagc ccggacgatc accgtggcga acggcttgac cggctcggcg ctggccacgg
     2881 cgattcaaaa cgccgtgcga gcgctggttc ccaacgatcc cttcaagggc gccgcctatg
     2941 caggattcac cgcatcggtc aacgcctcca acaccttcac attcgcgtcg ggcatgaccg
     3001 ggacgacgaa caattccacg accggcaccg ggacggtcgt agttgcggcg aacggcggcg
     3061 ataccttggc ggccaacctc aatatgctgg caccgacctc gacgaccggc acggacttct
     3121 tgctgtcgga cccgtctgcc acctcgaatt tttccacatc gatgacggtg tatgattcac
     3181 tgggcaacag ccatctgctg acgacctact tcacgaaggt cggcgagaac agttggaact
     3241 acaacactgt cgcagcggcg accgatgtgg tgacggccaa ctatcattcc agcaacatcg
     3301 ataccagcct gggcattgtc cgagtcgggt ccggcacctt gacgttcggg acagatggaa
     3361 cgctggatcg ggagagtacg gtaatccggt atgacaccgc cacggcggcc ggcacgtctg
     3421 ggacggcgcc gggagagttg caactggact ttgtcggcgc gacgcaagat cagccgatcg
     3481 ccatgaattt tggctccagc gtgacgaccg acggcggaag cgggctcgac ggcacgacgc
     3541 agttcgggtc gacgtcggcg ctcgtccagc agacacagga tgggtttgcc gccggctcgc
     3601 tccaagcctt ttcagtggat gcgaacggca ccattaacgg ccgtttctcg aacggtcaag
     3661 tgcgtgcatt ggcacaggtc gtgctcgcac gcttccccga tcccatcggg ctgacgagga
     3721 ccggaaagaa taccttcgcg cagtccggcg attccggcca gccggtcacc ggcacgcccg
     3781 acagtgccgg gctcgggcag gtcaaatcca actcgctgga attgtcgaac gtggatctcg
     3841 gcgagagctt catcgatatg atcgccgcgc agcgcgggtt tcaggcgaac tcgcgcgtga
     3901 tcaccacgtc cgacgaaatc cttcaagaac tggtcaatct gaaacggtag atccttcctc
     3961 tcgtggtggg gctggggcgc cggcgcgttc ccagcccgct ctccttgacc cacctactgc
     4021 ggcagttgcg actcgggacc cgtcatctct tatctgtcag cttattgacg cggtcaagcg
     4081 ggtttgcttg acaggggtgt tcatgcgctg tggtttcaac ggtacgtttc gtatcgaatt
     4141 gcgcgccaga tatggtgatt tgcgattgtt tcttcaggag agcagcaagg catagtggca
     4201 tatgcattgc agtctccgca ccgggccaat gagtcaatcg aggaggagtt atggcagatg
     4261 cagccgcagt cgatgaaaaa actccagtaa cggcccccgc tccggcgttc cccatcaagc
     4321 tactcattat tgtatcggtt gttgccttgg cgttcggcgt cggcggagca tttgtggtgg
     4381 tcaagtttct gggag
//
LOCUS       DATMTI010000019         1998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_255395, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000019 DATMTI010000000
VERSION     DATMTI010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255395"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1998
                     /locus_tag="VN039_00325"
     CDS             <1..>1998
                     /locus_tag="VN039_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44467.1"
                     /translation="VAYFTASTVITGTATDSTRTHALFATAGAPAFRAIAAGDIPTLN
                     QNTTGSAAKWTTARLLAGNSTDGSANVPFTNAFVVGGTADSGLTGAQFLGSLTTGLVK
                     NTTGAAPNLSIAANSDVIALWTGPCTSSNFLRGDGSCATPTSSGAINNSTQFSIPYYS
                     VVGTANTLSGIAGATAPNGVPQTLTSTPSGGVATIPALSLPGLGNRNVTGTTSTDTIL
                     STDCNPGRVDYQGSVAVATTLPTATTLGVPSCSFRIANNTSPAANVTVTPTTWTVNGS
                     TTQVIHSGQLCTFTVDAAGSAWDADCPNMGLVAGSNITITPGQFGPTISSTSTGVPGG
                     SNTQLQYNNSGSFGGTSGWTTNGTTTITGGSSSVLDLSAMAPTSGLKLPTVTGAVPTA
                     DSFIGTNSTTHTLVWGSNGTTIVGAAATTGTGTATTCTNQVVTAISALAAPTCTSLTS
                     AFLPSAVVYTNTTNTASSGMTLDMSGATGASAFRTPAQAGYTSAANGALGYDTTAGVA
                     HQRTAGADSLSASESSAITSNTIPKASDSTHAQLAASSLTDNGTTVTSTDPGGYVAPV
                     FVANGSTAGFADFPQGSTSASVAPCNTANSICEMAPTSVTSYTAVKPGAAANGILVGT
                     LSSSTLTQGFTGDTNHSTLVTIGSGTSIGSTSLCSSTFCPV"
BASE COUNT          443 a          739 c          454 g          362 t
ORIGIN      
        1 aagtcgctta cttcacggct tcgaccgtta ttacaggcac cgctaccgac tcgaccagga
       61 cccacgcctt attcgccacc gctggtgccc cggcctttcg tgccatcgca gcaggggaca
      121 taccgacact caatcagaac acaactggga gtgcagccaa atggacaacg gcccgcttgc
      181 tagctggcaa ttcgaccgat ggctcggcca acgtgccctt caccaacgcc ttcgtcgtcg
      241 gtggtacggc agactctggc ctgacgggcg cacaattcct cggctcgctc acaacaggcc
      301 ttgtcaaaaa cacaaccgga gcagctccca acttgtccat cgcggccaac tcagatgtga
      361 tcgctttgtg gactggccca tgcacatcgt ccaacttcct tcgtggcgac ggctcgtgcg
      421 ccacccccac ctcatccggt gcaatcaaca actcgaccca gttctcaatc ccttactact
      481 ccgtcgtggg caccgccaac acgctctctg ggatcgctgg cgcaacggcc ccaaacgggg
      541 ttccacaaac tctcacctcc acgccatcag gaggcgtcgc caccatacca gccctctctc
      601 tccccggcct tggaaatcgt aacgtcacag gcacaacctc cacggacaca attctctcaa
      661 ccgattgcaa tccgggccgt gtcgattacc aaggttcagt cgcggtcgcc acgaccctcc
      721 cgacggccac gacgttaggc gttccctcct gttcgttccg tatcgccaac aacacgtccc
      781 cggcagctaa tgtaaccgtc acacccacga catggacggt gaacggaagc acgacacagg
      841 taattcactc cggccaactc tgcacattca ccgtggacgc cgctggctcc gcatgggatg
      901 ctgactgccc aaacatggga ctcgttgctg gctcaaacat caccatcacg cccggacagt
      961 ttggcccaac catttcctcc acatcaacag gcgtccctgg tggctcgaac actcaactcc
     1021 aatacaacaa ctcaggttcc ttcggcggaa caagcggttg gacaacaaat ggcacaacca
     1081 caatcacggg tggctcgtca tccgtccttg atctctccgc catggccccg acctccggac
     1141 tcaagctccc aaccgtcaca ggggccgttc cgaccgccga cagcttcatc ggtaccaact
     1201 ctacgaccca tacgcttgtc tggggatcga acggtacgac catcgtggga gcggcagcca
     1261 ccacgggcac aggcaccgct acaacatgca cgaaccaagt cgtcaccgcc ataagtgcac
     1321 tcgccgcccc cacatgcacc tccctgacca gcgcatttct cccatccgcc gtcgtctaca
     1381 cgaacaccac aaacacagcc tcaagcggaa tgacgctgga catgagcggg gccacgggtg
     1441 cgagcgcatt ccgcacgccc gcgcaggctg gttacacatc cgccgccaac ggagcactcg
     1501 gctacgacac caccgcaggc gtcgcccacc aacgcactgc cggagctgac agtctgtcgg
     1561 ccagtgaatc atcggcaatc acatcgaata cgattcccaa agcctccgac tcgacccatg
     1621 cccaactggc cgcttcctcc ctgacagaca acggcacaac cgtcacttcg acagacccag
     1681 gtggctacgt cgcccccgta ttcgtcgcca acggctcaac cgcaggcttt gccgatttcc
     1741 cccaaggttc cacttccgcc tccgttgccc catgtaacac agccaacagc atttgcgaga
     1801 tggctccaac gtccgtcacg agctacaccg ctgtcaaacc gggtgcagcg gctaatggaa
     1861 tccttgtcgg aaccctcagt tcaagcacac ttacacaagg cttcacaggc gatacgaacc
     1921 attccacact cgtcactata ggctccggca catccatcgg ctcaacttct ttgtgttcct
     1981 ccacattctg tcccgtgg
//
LOCUS       DATMTI010000020         5213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_594930, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000020 DATMTI010000000
VERSION     DATMTI010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594930"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(535..1974)
                     /locus_tag="VN039_00330"
     CDS             complement(535..1974)
                     /locus_tag="VN039_00330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV44468.1"
                     /translation="MLQDADALPQLIGEYKPLDQWQTHLNQLFYGLRGDKLRSYYQTY
                     ASADFRLAHALAADYYERVTKREKTGNRQPSPADRIMDDESCLTVLELGPGNGNLAAC
                     FLSHLKTLDRQSTIYPRIRYVMVDWQQTVLDDALAHPDLARHRVQVETHQGSVEHLAG
                     IADASVDRIICNELWNDLPTKLLAKHGGEVEEEYLRPNLSESLHAKIQDWSAFVRAFQ
                     EKNLATLKTVPPFLDELVWEKEYRKVEWKDVPYRKTIAEFLQSIDQDVLVPVNVGAFA
                     TLKEAKRLLASDAIGLSVFDAGTGDMKVLNDPEKPCYGQFGGQYSFMINFALVESVAK
                     QLGMKQGTWEPQREFVGRSLNTNVITLMDLLATHPSAGPKLQAWEQDRLVLKTIKALN
                     ETFESPYHRRFDFPLTFNMPSEEREPLGVLLRSLKDNGIPDTVAYLTEEELTRAQKDL
                     EDIGYNSEAIRMALNAPPGPIEYCHFTCR"
     gene            2126..2686
                     /locus_tag="VN039_00335"
     CDS             2126..2686
                     /locus_tag="VN039_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44469.1"
                     /translation="MMNEVFRLGIVLAGSFLWCQAAAAEDLPLTENVPMSEFQNRTED
                     VRAYDFDAPPQGMFRSIALAEDFEERMGFQRTHEIMPVKPTDRFAPDVPAIFIVFSLH
                     QHYQGFKVFGRCAPEMVAGLAPETIVSEDAMHIALEDDSGYLKLSPPKGGWKPGHYKV
                     EIHVGEQINETSLMGTMRLTIVASGK"
     gene            2780..3532
                     /locus_tag="VN039_00340"
     CDS             2780..3532
                     /locus_tag="VN039_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWV44470.1"
                     /translation="MTSPSNPWASVIIPIKDERENLSPLLTGLLKVMDSHELSHSRPY
                     EILFVDDGSSDGSSDELDRLAREHPNMRVFHFDRNYGKTCALEAGFHQSSGEIIIQID
                     GDLQQDSEDILKLLPYTASHDVVCGWRQQRQDGLVRKFSSLIANRIRNLFTHDGMHDT
                     ACPLKIFRRPVLERIRLFEGMHRFFPALALMHGFTVTEVPVRHYPRIHGVSKYGMGNR
                     LFKSLYDLIAVRWMQDRVLLYKFRDGDATHRE"
     gene            3587..3877
                     /locus_tag="VN039_00345"
     CDS             3587..3877
                     /locus_tag="VN039_00345"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HWV44471.1"
                     /translation="MSTETIWIGIGFLGQGLFFGRWLIQWIASERSSESRVPIAFWYM
                     SLIGGLITLTYAIYRMDPVFISGQGLGSVVYLRNLVLIHRANQTKDQARPAA"
     gene            3874..>5213
                     /locus_tag="VN039_00350"
     CDS             3874..>5213
                     /locus_tag="VN039_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWV44472.1"
                     /translation="MSDWETNGHTGPSLTREQQPHPAMDRTPSQLIQLLLLLLLSCLL
                     FFLNLGGMGLTDRDEGRNAEAGREMFASGDLITPTFNGELRVAKPVFVYWLMTLSYHL
                     LGVNEFAARAPSALFGVALIVMHYLFLTRLRGPTVGLFGALMLLLNIEMLALGRMAIT
                     DSVLIFFATLSLYGFWLGLHEPGRGRHWIWAFYAGMALATLTKGPVGFAVPLITALLY
                     LAATRQWLSFWKKGAPLSGTLLFLMLAGPWYAAMFLVHGDAYSSQAKVHTVGRFLAPM
                     EGHGGGWWFYFPVLLLGFYPWSAFLPAGLYRAYQSWRESRMAGGHKHESPESEEPLGS
                     GNELEWFAGLWIVGVFIFFTLSSTRLPHYIGPLFPACALLAALYWAQGLKDPSTKGLR
                     GSIHFMMGSGYLLAIGFASLPSLYHKFSGKMTKEFPLAAQFDLGIGPYVGATIVV"
BASE COUNT         1079 a         1477 c         1414 g         1243 t
ORIGIN      
        1 cctctttttg ccaccctatt cacccgacct gaatcctatc gagcacgact tcgccgcgct
       61 caagaaacac tgggaatatc atgaaactgc ttccattgcc cagatcgtga aggcctatca
      121 atgattatcg gcttggctat agaccaccgg caacctccct ggtttgtgat gatcaagggg
      181 aggctcaata gcccaggccc agtcgaaata ctatctacag acgactgagc ttgaccgacc
      241 ctgagcttgc ctgccaccgc ttggccggac gatgtggagg accgcaatgg cccaagggct
      301 cttccgagtg atgtaggagc gactaaaatc gtcatcactg gagcatcacg tacggtggaa
      361 gcgggttaac ttatgcgcag tatggactcg cctatcgcta tgggacagga ctatccggca
      421 gcgactgggc gacactggaa ccggaagcgc gccggacatg ggaagaaaag agtccgccgc
      481 cctcattgtc tccttcaaca aaaggagggc gggcagccga ctaggattgc gttatcaccg
      541 gcaggtgaag tgacagtatt cgatgggacc gggcggggcg ttcaatgcca ttcttatcgc
      601 ctcgctattg taaccgatgt cttccagatc tttttgagcg cgggtgagtt cttcttcagt
      661 caggtaggcc acagtgtccg gaatgccgtt gtctttcaat gagcgcagga gtacgcccaa
      721 aggctcgcgt tcctcagaag gcatattgaa ggtcaacgga aaatcgaacc gacggtgata
      781 ggggctttcg aatgtctcgt ttaacgcttt aatggtcttc aacacaagcc tgtcttgttc
      841 ccaggcttgc agtttcggcc cggcggacgg gtgcgtcgcc aacagatcca tgagcgtgat
      901 cacattggta ttcaacgacc gtcctacgaa ttccctctgc ggctcccacg tcccctgctt
      961 catccccagt tgcttggcca cagactcgac caacgcaaag ttgatcatga agctgtattg
     1021 tccaccgaac tggccgtagc agggcttctc tggatcgttc aacaccttca tgtctccggt
     1081 acccgcatca aacacactga gaccaatcgc atccgacgcc agaagccgct tggcttcttt
     1141 cagggtcgca aaggcgccga cattgacggg aaccaagacg tcttgatcga tggactgcag
     1201 aaattccgcg atcgtcttcc gataaggcac gtctttccat tcaactttgc ggtattcttt
     1261 ttcccagacc agttcatcga ggaagggagg aacggtcttg agcgttgcaa gattcttctc
     1321 ctgaaaagcc cggacgaacg ccgaccaatc ttgaatcttg gcatggagcg actcgctgag
     1381 gttggggcgg agatactctt cctcgacctc gccgccatgt ttcgccagca atttcgtcgg
     1441 cagatcgttc cacagctcgt tacagatgat ccggtcgaca ctggcatcgg cgattccggc
     1501 caaatgctcg acagatccct gatgggtttc gacttgcacg cgatgtctcg ccagatcggg
     1561 atgtgccaga gcatcatcca gcaccgtttg ttgccaatcg accatgacat accgaatacg
     1621 tggatagatc gtgctttgtc tatcgagggt tttgagatga ctgagaaagc aagcggcgag
     1681 attcccgttc ccgggcccca gttccagaac cgtcaagcat gattcgtcat ccattatccg
     1741 gtcggcaggc gacggttgac gatttccagt cttctcgcgc ttcgtcacgc gctcgtaata
     1801 atcggccgcc agtgcatggg cgagccggaa gtctgccgag gcgtaggtct ggtaataaga
     1861 tcggagcttg tccccccgca atccataaaa aagctggttg agatgggtct gccactgatc
     1921 gagcggctta tattccccga tcagctgcgg aagggcgtca gcatcttgca acattctcac
     1981 gtctcacagt cactggtcat gattcatgat tgatgacttg gccgtacggt cggtgaaatc
     2041 ctaacagaag cccggaaaac gccgcaatgg ctaacttgac cgatagttta ttgacagcgc
     2101 cgtctcaagg gtgtagcaga caggcatgat gaacgaagtc ttccgtctgg gaatcgtgct
     2161 cgccggttcc ttcctgtggt gccaagcggc tgcagctgaa gatctgcctc ttaccgaaaa
     2221 cgtaccgatg tcggagtttc aaaatcgcac agaggacgtg agggcctacg atttcgatgc
     2281 gccgccgcag ggcatgtttc gctccattgc tttggcggaa gattttgagg agcgaatggg
     2341 atttcagcgt actcacgaaa tcatgccggt caaacccacc gaccgcttcg ctccggacgt
     2401 cccggctatt tttatcgtgt tctcgctcca tcaacactat caagggttca aggtcttcgg
     2461 ccgctgtgca cccgagatgg tagccggcct cgcaccggag accatcgtca gcgaagacgc
     2521 catgcacatc gcactggaag atgacagcgg ttacctcaaa ctgtctcccc caaagggcgg
     2581 ttggaaaccc ggtcactata aagtcgagat tcatgtgggt gagcaaatca acgagacgag
     2641 cttaatgggc acgatgcgtc ttaccatcgt ggcatccggc aagtagattg cagccgcacg
     2701 tttgaccctc cgccaaccat ccgctagaat gcgccactta ctaatccagt ctccggctgc
     2761 ggatcgagtt ttgcgtatca tgacgtcgcc atccaaccct tgggcctcag tgatcatccc
     2821 gatcaaggat gagcgcgaga acctgtcccc tctcctcacc ggcctcttaa aagtcatgga
     2881 ttcccatgaa ctgtcacact cccgtcctta cgagattctc ttcgttgacg atgggagcag
     2941 cgacggcagc agcgacgagc tggaccgatt ggcacgcgaa catcccaaca tgcgggtgtt
     3001 ccacttcgat cgaaattacg gcaagacctg cgcgcttgaa gccggctttc atcaatcttc
     3061 cggtgagatc atcatccaga tcgacggtga tctccagcaa gacagcgaag atatcttgaa
     3121 actgcttcct tatacggcat cgcacgacgt agtctgtggg tggaggcagc aacggcagga
     3181 cggcctggtg aggaaattct cctcattgat cgccaaccgc atacggaatc tcttcacaca
     3241 tgacggcatg cacgacacgg catgtccgct caagattttc cgacggcctg tgctggagcg
     3301 catccgactc tttgagggga tgcaccgctt ctttcccgcg ctggctttga tgcatggatt
     3361 caccgtgacg gaggtgccgg tcagacatta tccgcgaatc cacggagtgt ccaagtacgg
     3421 catggggaac cgcctgttca aatcgctcta tgatctgatc gcggtccgat ggatgcagga
     3481 tcgcgtgttg ctctacaaat tccgtgatgg tgacgcgacc caccgcgaat gagactgaga
     3541 ccttcacgtt ccggaacgac gatgacgact gctctcccga cacctcatga gtactgagac
     3601 catctggatc ggtatcggct tcctcggcca agggctcttt ttcggtcgtt ggttgattca
     3661 atggatcgca tccgagcgga gctctgaaag ccgtgtgcct attgcctttt ggtacatgag
     3721 tctcattgga gggttgatta cgctcaccta tgcgatctac cgcatggacc ctgtttttat
     3781 ttctggacaa gggctcggat cggtcgtcta tctacgcaat ctcgtcctca ttcaccgggc
     3841 caatcaaacc aaagaccagg ctcggccggc agcatgagcg actgggaaac caacggccat
     3901 accgggcctt ccctcacgcg cgagcaacag ccacatccgg ccatggatcg taccccatcg
     3961 caacttatac agctgctgct ccttttgctt ctgtcctgtc tgcttttctt cctgaatctc
     4021 ggcggcatgg gtctgacgga tcgcgacgaa ggcagaaatg cggaggccgg tcgcgagatg
     4081 ttcgcctcgg gcgacctgat cactccgacc ttcaatggcg agctgcgtgt tgccaaaccg
     4141 gtctttgtgt attggctgat gacgctgtcc tatcatctgc tcggcgtgaa cgagtttgcg
     4201 gcgcgggcac cctcggccct gttcggagtg gcgttaatcg tgatgcacta tctgtttctc
     4261 accaggctgc gcggtccgac cgtcggtttg ttcggcgctc tgatgttgct gctgaacatc
     4321 gagatgctgg cactggggcg catggcgatt actgatagcg tcttgatctt cttcgccacc
     4381 ctgtcgctct acggattttg gctcggcctc cacgaaccgg gcagggggcg tcattggatc
     4441 tgggcctttt atgccggcat ggccttggca acattgacga aagggccggt gggattcgcc
     4501 gttccattga tcactgctct cttgtatctc gctgccactc ggcaatggct gagcttttgg
     4561 aagaaaggcg cgcctctctc cggcacgttg ctcttcctta tgctcgcagg tccctggtat
     4621 gcagccatgt tcctcgtcca tggagatgcg tactcctctc aagcgaaagt ccacacggtg
     4681 ggacgattcc tcgctccgat ggagggacat ggagggggct ggtggttcta tttccctgtt
     4741 ttgctgttgg gcttttaccc ttggagcgcg ttcctaccgg cagggcttta tcgcgcctat
     4801 cagagctggc gggaatcccg catggcagga ggccacaagc acgaatcgcc ggaatctgag
     4861 gaaccgctag gatccggtaa tgagttggaa tggtttgcgg ggctgtggat cgtgggcgtg
     4921 ttcatcttct ttactctgtc gtctacccgt ctgcctcact atatcggtcc cctgtttcct
     4981 gcctgcgcct tgctggccgc cttgtattgg gctcaaggct tgaaagatcc ttctacaaag
     5041 ggactgcgtg gatctatcca ctttatgatg gggagcggct atctcttagc gatcgggttt
     5101 gcaagcttgc cctccctgta tcacaaattt tcggggaaga tgaccaaaga gttcccgctt
     5161 gccgctcaat tcgatctggg tatcggtcct tacgtcggcg cgacgatcgt cgt
//
LOCUS       DATMTI010000021         3720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_637322, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000021 DATMTI010000000
VERSION     DATMTI010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637322"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..908)
                     /locus_tag="VN039_00355"
     CDS             complement(join(151..567,570..908))
                     /locus_tag="VN039_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611879.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWV44473.1"
                     /translation="MRRYGLRDDQWEKIEHLLPGREETVGVTAKDNRLFVEAVLYRYR
                     AGIPWRDLPERFGDFRVIHTRHTRWSQRGVWKRVFERLADDPDNEYAMIASTIIRAHQ
                     HSAGAKGGTGRECIGRSKGGLTTKIHARCDALGNPTGFHLTPGPAHELEGTDALLPGM
                     TADTRIADKAFDADERVIEPLRKAGKTIVIPSKSNRTVKRSYDEEFYKARHLIENFFA
                     RLKQFRAIATRYDKRAANFLGGIYLAASMTWLN"
     gene            complement(1406..2497)
                     /locus_tag="VN039_00360"
     CDS             complement(1406..2497)
                     /locus_tag="VN039_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44474.1"
                     /translation="MPLIWAAISGHGFGHAAQVVPVLNALHRLVPDLRVLLRTAVPPS
                     FFQDRLVPPWDVSSVQQDVGCVQNGPLKIDVEATWREHHRFHSSWEARFQTEIEAMKT
                     ASPNVVLADTPYLALAAGKAAALPAVALVSFTWDLVLSEYIAPRSIDGRAIIQSIRQA
                     YGKADLALRINPAPKMEIFQRIIDIGPIAEPASPAREQLTEFLGLARGEQTVLVGFGG
                     VPLDSLPFSRLESLTGYRFIFDGSIPLGSRRFVAAASLPFSFKTLMASVDLIMTKPGY
                     GTLVEAVALRTPLVYVRRYNFADEQPLVDYLHRYGRGVELSLDAFAHGHWEESLQHAQ
                     SLPPPSEHVPPATGADEAAALLASHFFHG"
     gene            complement(2550..2750)
                     /locus_tag="VN039_00365"
     CDS             complement(2550..2750)
                     /locus_tag="VN039_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44475.1"
                     /translation="MNLDDARKRIESAVTQYGQHAAPAIDLVMAEVRSDLGKGAFNEL
                     IEEFDLELMYNITPMESDQSNS"
     gene            complement(2782..3096)
                     /locus_tag="VN039_00370"
     CDS             complement(2782..3096)
                     /locus_tag="VN039_00370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV44476.1"
                     /translation="MDIDAFRQMVAKNPKGFLGRYGLGNKILQENGSVEEAVEHLTVA
                     TQLDPTHAASHLALGRALIRLGRDADAKRILTAGIEAVLSGRSNGGKDLVPEMQELLR
                     SL"
     gene            complement(3187..>3720)
                     /locus_tag="VN039_00375"
     CDS             complement(3187..>3720)
                     /locus_tag="VN039_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44477.1"
                     /translation="ERRILSSIQETSLFSTLMPLGEKPDSLGDKVVSARITVDETIDP
                     HSWLSAFKGIIIGGSMFLLSPFIDLEYGYAATVALELERWDGQVTRYEARSSGSVHYK
                     LFGATPVMIDELKGHVTEACLSDLTGQLVRDTKLYMASSAPLPDTGIRTVTVKAKRPA
                     GTQPTRSLVPVATATTP"
BASE COUNT          850 a         1018 c         1040 g          812 t
ORIGIN      
        1 tccaaggaac acaattagcg ggcgtttatc tatgattttc gataggaatt catcacacgg
       61 gttggagggc gtaagttcgc ttcccggctc aagccactct tcatactgtt caagcctgac
      121 ctgtagatgt atccaaacta aggtgtgtca tcaattaagc catgtcattg aagcggcgag
      181 atagatcccg ccgaggaaat tagcagcgcg tttatcataa cgcgtggcga tggcgcggaa
      241 ctgcttgagt ctggcgaaga agttctcgat cagatgccgc gccttgtaga actcttcatc
      301 gtatgaccgt ttgaccgttc tgttcgattt tgacgggatc acgatagttt ttcctgcttt
      361 tcgtagcggt tcgatgaccc gctcatcggc atcaaacgct ttatcggcaa tgcgtgtgtc
      421 cgcggtcatg ccgggcagca gcgcatcagt cccctcaagc tcatgtgccg gacctggcgt
      481 cagatggaag cccgtgggat tacccagcgc atcacaccgg gcatggattt tcgtggtcaa
      541 tccgcctttg gagcgcccga tgcattccgc cgtccggtcc ccccttttgc cccggcactg
      601 tgctggtggg cgcggatgat cgtggaagca atcatggcgt attcgttgtc aggatcatcg
      661 gccagacgct caaacactcg cttccagaca ccgcgttggc tccagcgcgt atggcgtgtg
      721 tggatgaccc ggaaatctcc gaaccgctcc ggcagatccc gccacggtat cccggcccgg
      781 taccggtaca acaccgcctc gacaaacagc cgattatcct tcgccgtcac gccgacggtc
      841 tcttcacggc ctggcagcag atgctcgatc ttctcccatt ggtcatcgcg caatccgtac
      901 cgtctcacca tccaaaggcc ctccttcggc ctatgaatag ggaatcacac cgtcattgcc
      961 ttgtgaatca ggaaaattaa ttgatgacac gccttagctg agtaaagaaa tactccggtt
     1021 atgaagacaa gtactaaaca actgccaggt ataaaccttc agactcggaa cccaccccgt
     1081 ccctgctccg cgatgttgcg tccaaatagc acgccttttc tcaagcaaat actcactttg
     1141 agccatgcta tgattaccac caatccagca actaacaacg tcgcataggc cgtccagaat
     1201 aacagccaag cccatgttgg ccacgcctgc gcgaacacta tgcaacccca cccggtcgca
     1261 acgagtgccg caagaatttt ccatataacc atgcccccat ccacttgaaa gtgggcgcat
     1321 tctagggagg gtctgccgtg tggtgtcaag aaggttgcaa tttccaagaa taatggcggt
     1381 tatccgtaca cgcatcggtc aatgtctagc cgtgaaaaaa atgggaagcg aggagtgctg
     1441 ccgcctcatc ggctcccgta gccggaggaa cgtgctcgga gggaggggga agcgattgcg
     1501 catgttgcaa agactcctcc caatgaccat gagcgaaagc atcgagtgac agttcaacgc
     1561 cccgtccgta ccggtgcaaa tagtcaacca gtggctgctc atcggcgaaa ttgtatcgac
     1621 ggacatatac cagaggagtg cgtagcgcga ctgcctccac caaggtgccg tagcccggtt
     1681 tcgtcatgat gagatcgaca gacgccatta aggtcttgaa tgaaaaaggc agggatgcgg
     1741 cagcgacgaa tcgacgactt cccagtggaa tcgagccgtc gaagatgaaa cgatatccgg
     1801 tgagtgattc cagccggctg aatggaagtg agtccagggg aactccacca aatccgacga
     1861 gcacggtttg ttctccacgg gccagcccta ggaactcggt caattgttcc cgagcgggcg
     1921 atgcaggctc ggcgatcggt ccaatgtcga tgatgcgttg gaagatctcc attttgggag
     1981 ccggattgat ccgcagcgcg agatctgctt tgccgtaggc ctgtcgaatc gattggatga
     2041 ttgcccggcc gtcgatcgat cgaggggcta tatactcgga cagcacgaga tcccaggtaa
     2101 aactgacgag cgcaacggca ggaagagcag ccgcttttcc cgcggctaaa gccaagtagg
     2161 gagtatcggc caagacgaca ttcgggcttg cggtcttcat cgcttcgatt tccgtctgaa
     2221 accgtgcttc ccaagaactg tgaaaccgat ggtgctcgcg ccaggtggct tctacatcga
     2281 tcttcagggg gccattctga acgcatccga catcctgctg aacggagctt acgtcccacg
     2341 gaggaacgag acggtcttgg aagaaggaag gagggacagc cgtgcgcagg aggacgcgaa
     2401 ggtcaggaac gagacgatgt aacgcattca gcaccggcac gacttgcgcg gcgtgaccaa
     2461 atccatgtcc tgagatggca gcccagatca gcggcatagg caggagactg tgcgagtcac
     2521 gagtgaacgg tggaatagga agatactgtt caactatttg actggtccga ttccatcgga
     2581 gtgatgttgt acatcaactc aagatcgaac tcttcaatca gttcattgaa cgctcccttt
     2641 cccaaatcgg agcgaacttc tgccataacg aggtcgatgg cgggggcggc gtgttggccg
     2701 tattgtgtga cggctgattc aattcgtttt ctggcatcgt cgagattcac ggcgaaactc
     2761 cttatggtac aagttcatac gtcatagcga gcgtagcagc tcctgcatct caggcaccag
     2821 atccttgccg ccgttcgatc gtcctgaaag tacggcctcg ataccggcgg taagaatacg
     2881 cttggcatcg gcgtccctgc ccagtcggat cagcgcgcgt cctaacgcca gatgagaggc
     2941 ggcatgtgtc gggtccaatt gtgtggcgac agtaagatgc tcgactgctt cttcgacgct
     3001 accgttttcc tgaaggatct tattgccaag cccataacgg ccgaggaatc ccttgggatt
     3061 cttagcgacc atctgacgaa aagcgtcaat atccatcaat cgtggttcca gtgcccaggc
     3121 gtcactatac cactggaacg gcaggatcgg ccagatgaga acgttggttg ggggaactcc
     3181 gtgttcctac ggcgtcgtag ccgtcgcgac gggaacaaga gaacgggttg gctgtgtgcc
     3241 ggctggtctt ttggctttca ccgttaccgt gcgaatgccg gtgtccggga gcggggcgct
     3301 gctggccata tagagtttgg tgtcacgaac aagttgtccc gtcagatcac tgaggcaggc
     3361 ttccgttacg tgtcccttga gttcatcgat catgaccggc gtggcgccga acaacttgta
     3421 atgcaccgaa ccggaggaac gggcttcgta gcgtgtgact tgaccgtccc aacgttcgag
     3481 ttcaagggcg accgttgcag catacccata ttccaggtcg atgaagggcg agaggagaaa
     3541 catggacccg ccgatgatga tacctttgaa cgccgagagc caggaatggg gatcgatggt
     3601 ttcgtcgacg gtgatgcgcg cggagacgac cttatctccg agcgaatcgg gcttttctcc
     3661 caacggcatc aatgtcgaaa acaggctggt ttcttggatg ctgcttaaga tccgccgctc
//
LOCUS       DATMTI010000022         1716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_213144, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000022 DATMTI010000000
VERSION     DATMTI010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213144"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VN039_00380"
     CDS             <1..268
                     /locus_tag="VN039_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWV44478.1"
                     /translation="GYLLARSARQITIAQILEGIEGPLGITDCSHEKDGEFCMQREHC
                     NIRTPLLKIQDSIYQLLNNMTLGDMLGGTPLITIQSPSAQGVER"
     gene            265..1482
                     /locus_tag="VN039_00385"
     CDS             265..1482
                     /locus_tag="VN039_00385"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HWV44479.1"
                     /translation="MKLPIFLDNHSTTPMDPRVLETMLPYFVEKFGNSASRNHAFGWA
                     AEEAVEQARKQIAKLIKADSKEIVFTSGATESDNLALKGVLEMYKEKGSHIITSSAEH
                     RAVLDTAKSLEAKGLATVTYLPVDKYGMVNPQDVQNAITDKTILISIMLANNEIGTIN
                     PVQEIGKIAKARGVLFHCDATQGVGKIPVDVQAMGIDLMSFSAHKIYGPKGVGALYVR
                     KRNPRVRIAAQMDGGGHERGMRSGTLPVPLIAGFGKACEICEQEMTAEAARLTKMRDR
                     LQTDIMAALEESYLNGHPTNRLPGNLNISFAYVEGESLLMGMKEIALSSGSACTSATL
                     EPSYVLRALGVGTELAHSSIRFGLGRFNSDEEIDYTIKKVIEIVTKLREMSPLYEMAK
                     EGVDLKSVQWAAH"
     gene            1515..>1716
                     /locus_tag="VN039_00390"
     CDS             1515..>1716
                     /locus_tag="VN039_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWV44480.1"
                     /translation="MAYSDKVVDHFNNPRNMGSFKKDEEGVGTGMVGAPECGDVMKLQ
                     IKVQDDTIVDAKFKTFGCGSAIA"
BASE COUNT          428 a          460 c          489 g          339 t
ORIGIN      
        1 gggctatctt ctggcacgaa gcgcccgaca aatcaccatc gcacagattc tcgaaggtat
       61 cgaaggtcct ctaggcatca ccgactgctc gcacgaaaaa gacggcgagt tctgtatgca
      121 gcgcgaacac tgcaacatcc gcacgccgct gctgaaaatt caagacagca tctaccaatt
      181 attgaacaac atgacgttgg gagacatgct gggcggcacg ccccttatta cgattcagtc
      241 cccctcggca caaggagtcg aacgatgaag cttcctattt ttctcgataa ccattccacc
      301 actcccatgg atccgcgagt tctggagaca atgctgccct atttcgtgga aaagttcggt
      361 aactccgcca gtcgtaatca tgccttcggc tgggctgcgg aagaagcggt ggaacaagcc
      421 cgaaaacaga ttgcgaagct catcaaggcc gactcaaaag aaatcgtctt caccagcggc
      481 gccactgaat cggacaacct ggccttgaag ggcgtcttgg agatgtacaa ggagaaaggc
      541 tcccacatca tcacgtcgtc cgcggaacat cgggccgtgc tcgatacggc taagtccctt
      601 gaagccaaag ggctggcgac cgtgacctat ctgccggtcg acaaatacgg catggtaaat
      661 ccgcaggacg tgcaaaatgc cattaccgac aagacaatcc tgatctcgat catgctggcc
      721 aataacgaaa tcggcacgat caatcccgtc caggaaatcg gtaagattgc gaaagcaagg
      781 ggggtgctgt tccattgcga tgccactcag ggtgtcggga aaatcccggt cgatgttcag
      841 gccatgggca tcgatctcat gtcgttctcg gcccacaaga tttacggtcc taagggagtc
      901 ggggcgctct acgtcagaaa gcggaacccc cgcgtacgca tcgcggccca gatggacgga
      961 gggggacatg aacgcggtat gcggtccggc accttaccgg ttccgctgat cgcgggattc
     1021 ggaaaggcct gtgaaatctg tgagcaggag atgacggcgg aagcggcgcg gctgaccaag
     1081 atgcgagacc gcctccaaac cgacattatg gcggcgcttg aggaaagcta cctcaacggc
     1141 cacccgacga accgtttgcc gggcaatctc aacatctcgt tcgcctatgt cgagggggag
     1201 tcgctgctga tgggcatgaa ggaaatcgcg ctctcatccg gatcggcctg tacgtcggct
     1261 acactggaac cctcatatgt attgcgcgca ctcggcgtcg ggacggagct cgctcactct
     1321 tcgatccgct tcggcttggg ccgtttcaac agcgatgaag agattgacta tacgatcaag
     1381 aaggttattg agattgtcac caagttgcgc gaaatgtccc cgctctatga aatggcaaaa
     1441 gagggcgtgg acttgaagtc cgttcagtgg gcggcacatt aactgagata caacgtctat
     1501 tttcggagga caccatggca tacagcgata aggtcgttga tcatttcaat aacccccgta
     1561 acatgggaag tttcaagaag gacgaagaag gggtaggtac cgggatggtg ggggctccgg
     1621 agtgcggcga cgtgatgaag ctgcagatca aggtacagga cgatacgatc gttgatgcca
     1681 agttcaagac cttcggctgc gggtcggcga tcgcca
//
LOCUS       DATMTI010000023         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_43398, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000023 DATMTI010000000
VERSION     DATMTI010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43398"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..155)
                     /locus_tag="VN039_00395"
     CDS             complement(6..155)
                     /locus_tag="VN039_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44481.1"
                     /translation="MGAWAIIGIGFDFALFAAIGVLLFATFAQMDKEAMEEEWEEEER
                     NANAE"
     gene            complement(222..581)
                     /locus_tag="VN039_00400"
     CDS             complement(222..581)
                     /locus_tag="VN039_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44482.1"
                     /translation="MNIKATIGSHAPAERANEFVQVIDALIEEENNRTKAAAEKGGPV
                     FVQPNISFAVEAEKVGSVKRQMREAAHLRERTARLTAETVHPSGKKDVPDEIELTYIL
                     TDRITRTTGDETETATE"
     gene            complement(623..2593)
                     /locus_tag="VN039_00405"
     CDS             complement(623..2593)
                     /locus_tag="VN039_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44483.1"
                     /translation="MILAIGNAEPMGKAARDKRHYEKHKAAENRAAIDYQYLTRKFVA
                     IDGEGLNVRSGKRKGAHDYVLLAISDAEPILRREGLRTFEILDYLWRTLDPRNLNVIY
                     GGSYDFNCWLADLPQDVLQRIYEGGRRGKGFVYGGYRIKWMKGKSFSIKKDGKTVTIY
                     DVISFFQKSFIDACDEYLGTEWIGREVIVREKKRRGNFTWREIKNVGKYNNLELVNLV
                     RLCEELRIRLNGAGLRPKSWIGPGAVASALMSREGVKAHMSRDIPEEVARAARFAFAG
                     GRFEDLKYGMSRETAYEYDLNSAYPKALSLVPSLAGGKWRNVNRPAEIVPYGLYRVRW
                     ESAPDADPRVPGPLFHRGSNGTISYPMRGENWVWSPEVQNLAEYSTLTGLKYTLLEAW
                     EFEPATSHKPFGFVPALYERRKVLKAAGDGAHVGVKLGLNSLFGKTCQQIGWDKVKKT
                     APVWHQLEWGGFVTSWCRAQVLKAAMQDLDAVIAFETDALFTSRPLNLSVSERLGEWE
                     ETRFRSLTYVQSGHYYGTAWNEKKQAWEEVAKSRGVDRGELTRERVEEYFHLPLGQRM
                     LKAKLTRFVGIGIALSQSFARWRMWEVLTKELHLWPMGKRVPLEDRASGWSRTICPIV
                     GGMSHEYPVEWINANPEMSELADRRESEYDWE"
     gene            complement(2590..>2912)
                     /locus_tag="VN039_00410"
     CDS             complement(2590..>2912)
                     /locus_tag="VN039_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44484.1"
                     /translation="RENRSHWQQVKRGGLPDNFPTFLHSYVEPKGVSTAQWVGYIVSY
                     FHAIVDEKHNWDSVRDESGRWIIDERDGRVVPKSDIWWYRHFVIYSDKRSANYYETRY
                     GYAV"
BASE COUNT          550 a          908 c          736 g          718 t
ORIGIN      
        1 cagccttact ccgcgttcgc attgcgctct tcctcttccc attcctcttc catcgcttcc
       61 ttgtccatct gcgcgaacgt tgcgaagagc aacactccga ttgcagcgaa caaggcgaag
      121 tcgaatccga ttccgataat ggcccatgct cccattgcat tcctccttat atacgcgaaa
      181 aggccggggc tgatgcccgg ccttttcgat ggtgcggtgt gttactcggt cgcagtctcg
      241 gtctcgtcgc ccgtggtgcg ggtgattcgg tccgtgagga tgtaggtgag ttcgatctcg
      301 tcaggcacat ccttcttgcc ggacgggtgc acggtctcgg ccgtgagccg ggcagtgcgc
      361 tcgcgaaggt gcgcagcctc gcgcatctga cgcttgacgc tgccgacctt ctccgcctcg
      421 acagcaaacg aaatgttcgg ctgcacgaag acggggccac ccttctcggc ggctgctttc
      481 gtgcggttgt tctcttcctc gatcagtgca tcgatgacct gcacgaattc gttcgcgcgc
      541 tcggctggtg cgtggctgcc gatggttgcc ttgatgttca cgattgtttc tcctttgtaa
      601 gtcgggctgc gatgcctctt tatcattccc aatcgtactc actttcgcgg cggtcggcaa
      661 gttccgacat ctccgggtta gcgttaatcc attccacagg gtattcgtga gacatgccgc
      721 cgacgatggg gcagatcgtg cgggaccatc ccgaagcgcg atcctctaac ggtacgcgct
      781 tccccatcgg ccacagatgt agctcctttg tcaacacttc ccacatgcgc cagcgtgcga
      841 atgattggga cagcgcgatt ccgattccca cgaaccgcgt cagcttcgct ttcaacattc
      901 gttgtccgag cggtagatgg aagtattctt ccaccctctc acgtgtcagt tcgccccggt
      961 caacaccgcg agacttcgct acttcctccc acgcttgttt cttctcattc catgccgtcc
     1021 cgtaatagtg cccggattgc acatacgtga gggagcggaa acgggtttcc tcccattctc
     1081 cgagccgttc cgacaccgac aggttcagcg ggcgagaggt gaagagcgca tcggtttcga
     1141 atgcgatcac agcatccaag tcttgcatcg ccgctttcaa cacttgcgcg cgacaccatg
     1201 acgtgacgaa tcctccccat tcgagttgat gccagacggg cgcagtcttc tttaccttgt
     1261 cccacccgat ttgctgacac gtcttcccga acaaactatt gagcccgagc ttcacgccta
     1321 cgtgcgcccc atcgcccgct gcttttaaca ccttccgacg ttcatagagt gcgggcacga
     1381 atccgaacgg tttatgactc gtcgccggtt cgaattccca cgcctctagc agagtgtatt
     1441 tcagcccggt tagtgtcgag tattccgcaa ggttttgcac ctcgggtgac catacccaat
     1501 tttcgccgcg catcggataa gagatcgtcc cgttgcttcc cctgtgaaac agcgggccgg
     1561 gcacgcgcgg gtcggcatcg ggcgcggatt cccagcgcac ccggtacagg ccataaggca
     1621 cgatctcggc cgggcggttc acgttccgcc atttcccgcc tgctagtgac ggtaccaaac
     1681 tcaacgcttt cggatatgcc gaattgagat cgtattcata tgccgtttcc cttgacatgc
     1741 cgtatttcaa atcttcgaag cgcccgcccg cgaacgcgaa ccgagccgcc cgcgctactt
     1801 cctccgggat atcgcggctc atgtgcgcct tgacgccctc acgcgacatc agcgccgatg
     1861 caaccgcgcc aggaccaatc cacgacttcg gacgtaaccc ggcaccgttc agacggatgc
     1921 gcaactcttc acatagtctg acaaggttga ccaattcgag gttgttatac ttgccgacat
     1981 tcttgatttc gcgccacgtg aaattgcctc tccgcttctt ctcgcggacg atgacctcgc
     2041 gaccgatcca ctctgttcct aagtattcgt cgcacgcgtc gatgaatgac ttctggaaga
     2101 aagagatcac atcgtaaatg gtgactgtct tcccatcttt cttgatggag aagcttttcc
     2161 ctttcatcca cttgatacga tagccgccgt acacgaatcc ttttccccgg cgtccgcctt
     2221 cgtagatgcg ttgtaagaca tcctggggga gatcggctaa ccaacaattg aaatcatagc
     2281 ttccgccgta gatgacattg aggttacgcg ggtcaagggt gcgccacaga tagtcgagga
     2341 tttcgaacgt tcgtaagccc tcgcgtcgca agataggttc cgcatccgag atcgcgagta
     2401 acacataatc gtgcgctccc ttgcgtttcc cgctacgcac gttgagccct tcgccgtcga
     2461 tagcgacgaa ctttcgggtg aggtattggt aatcgatagc ggccctgttc tcggccgctt
     2521 tgtgcttttc gtagtgccgc ttatctctcg cggctttccc catcggttca gcgttcccaa
     2581 tcgcaagaat catactgcgt atccatatct tgtttcatag tagtttgcac tccgcttgtc
     2641 ggaatagatc acgaagtgtc tgtaccacca tatgtctgac ttcgggacga ccctgccgtc
     2701 acgctcgtca atgatccaac gcccggactc gtcacgcacg ctatcccagt tgtgcttttc
     2761 atccacaatc gcatggaagt acgaaacgat gtaaccgacc cattgcgcag tgcttacacc
     2821 cttcggttct acatacgagt gcagaaaggt cgggaaattg tccggcaggc ctccccgctt
     2881 cacttgctgc cagtgagacc tgttctcgcg gc
//
LOCUS       DATMTI010000024         3395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_298009, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000024 DATMTI010000000
VERSION     DATMTI010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298009"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..107)
                     /locus_tag="VN039_00415"
     tRNA            complement(18..107)
                     /locus_tag="VN039_00415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Ser,seq:cga)
     gene            complement(230..1417)
                     /gene="thiI"
                     /locus_tag="VN039_00420"
     CDS             complement(230..1417)
                     /gene="thiI"
                     /locus_tag="VN039_00420"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HWV44485.1"
                     /translation="MRCVIAHYHELALKGNNRDYFEQCLIKNIRTALKDVGVRQVENL
                     HSRIRVRLPPEAGPEVVQDRLKRVCGIANFLLGRMVPLELAAPNLDALTTAVLEEIES
                     QSFTTFRVTARRADKRLTLTSMDIEKALGAAVCDRTGKNVSLKNPDLTIYVELLSKEA
                     FCSTEKIEGPGGMPVGVSGKIACLISGGIDSPVAAYRMVKRGCLASFIHFSGRPLVSR
                     ASEEKVHDLVRQLTSFQYNSRLYVIPFGEIQREIILSAPPPFRVVLYRRMMIRIAQEL
                     ARKEQCWALVTGDSLGQVASQTPQNLRAIEEAAELPILRPLIGMDKREIIDEARRLGT
                     YETSIEPDQDCCKLFVPPHPSTKTRLDDVQKVERMIDVSALVKRGVENAELTELSFPP
                     STA"
     gene            complement(1480..2403)
                     /gene="trxB"
                     /locus_tag="VN039_00425"
     CDS             complement(1480..2403)
                     /gene="trxB"
                     /locus_tag="VN039_00425"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWV44486.1"
                     /translation="MRNVVIIGSGPAGLTAAIYAARANLAPLLVEGWQGGGQLTTTTE
                     VENYPGFAKGIMGPELMKEMRSQAERFGTVFKTGDATSADLQTRPFKVIVDGEETFET
                     KTLIIATGASPITLGLTNEKRLWGYGVSSCATCDGFFFKGKELVVVGGGDSAMEEATF
                     LTKFATKVSIIHRRDKLRASKIMQDRAMKNEKIAFIWNSVVEDVLGQDVVTGVRIRNV
                     ITGKVWDLHCAGFFLAIGHRPNTALFTGQLKMDGAGYILTNHRTATNVSGVFAAGDVQ
                     DSHYRQAITAAGTGCMAAMDAERFLETSSQG"
     gene            complement(2444..3019)
                     /locus_tag="VN039_00430"
     CDS             complement(2444..3019)
                     /locus_tag="VN039_00430"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HWV44487.1"
                     /translation="MTHPPERESLENFLRTILDALPTPPPIYCLVGALALGAWGRIRS
                     TKDIDLLMVAEELQRARLMACLREKGFHIDQNWLDHNPFAADRVNRLFHTSFHDIPLD
                     VIFVADRHEEETLRRRRPFMIVGLQTWVCGPEDLILMKLKASRGHDFEDAISIMLNPN
                     LQLDFDYLWSWAERLGLQSELHYVLISADPK"
     gene            complement(3003..3251)
                     /locus_tag="VN039_00435"
     CDS             complement(3003..3251)
                     /locus_tag="VN039_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44488.1"
                     /translation="MPIDPDDPPIWLRKLHVSAAQLSPSDYSATPEEGILQVCRLSDA
                     HRVLERAMKQALGHAPLPVPPPFEPPARFLHDHNDTSA"
BASE COUNT          755 a          899 c          945 g          796 t
ORIGIN      
        1 acacacgaag tgcggtatgg cggagagggt gggattcgaa cccacggtcg agttaccccg
       61 acagcagttt tcgagactgc ccgattcggc cgctctcgca cctctccgtt atggcagatt
      121 gtcggctggt ttgatgatcg agcaggcgag cttaccgtgg tcgatgcagt ttttcaatgg
      181 cttcacgtga ctccacgaga cttggcctga aatagaaaat gtgtgcgagt tacgcagtcg
      241 aaggggggaa cgacaactcg gtcaactccg cgttctccac ccctcgtttt accaaggcgg
      301 agacatcgat catgcgttcg actttttgca catcgtcaag gcgggttttc gtgcttggat
      361 gaggcggcac aaataacttg cagcagtcct ggtctggctc tatcgacgtt tcgtacgtac
      421 cgagacgtct ggcttcatcg atgatctcgc gcttgtccat gccgatcagt ggtcggagga
      481 tcggcagctc cgctgcttct tcaatcgcac gcaaattctg aggcgtttgg gaggcgactt
      541 gacccaggct atcgccggtc actaaggccc aacactgctc ttttctcgct aactcctggg
      601 cgattcgaat catcatccgt ctgtatagta cgacgcggaa cgggggaggt gcgctcagga
      661 tgatttcccg ctggatttcc ccaaaaggaa tcacatagag gcgtgaattg tattgaaagc
      721 tggtgagctg tcgcaccagg tcgtggactt tttcttccga ggctcggctg accaacggcc
      781 gacccgaaaa gtgaatgaaa gacgcaaggc agccgcgctt taccatgcga taggcagcca
      841 caggtgagtc gatccctcct gaaatcaaac aggcgatctt tccgcttacg ccgaccggca
      901 tgccgccggg accttcgatc ttttccgttg aacagaacgc ttccttcgat aggagttcga
      961 cataaatggt caagtcagga ttcttcaaac tgacgttctt tccggttctg tcgcagaccg
     1021 ctgcaccaag cgctttttcg atgtccatcg aagtcaacgt caggcgcttg tcggcccgtc
     1081 tggccgtcac cctgaacgtg gtgaaggatt gcgattcaat ctcttcgagg acagccgttg
     1141 tgagcgcatc caggttggga gcagccaatt cgagtggaac catacggccc agtaaaaagt
     1201 tggcgattcc acacacccgc ttgagccggt cttgaaccac ctctgggccg gcttcgggtg
     1261 gaagccggac gcggatgcga ctgtgaagat tctcgacctg ccggactcca acatctttga
     1321 gagcggtccg aatgttctta atgaggcatt gctcgaagta gtctcgattg ttccccttta
     1381 gagcgagctc gtggtaatgg gcgatgacgc aacgcatact cgagataggt cgaggttaag
     1441 gtggaaggtc tttctttcct taaaagctca gccgtagcct caaccttggc ttgaagtttc
     1501 taagaaccgc tcggcgtcca tcgcagccat gcagccggta ccggctgcgg tgatggcttg
     1561 gcggtagtgg gagtcttgta catccccggc cgcaaacaca ccggagacat tggtggctgt
     1621 tcgatgattc gtcaggatat agccagcgcc gtccatcttc agctggccgg tgaagagtgc
     1681 ggtgtttgga cggtggccga tcgccagaaa gaacccggca cagtgcaaat cccacacttt
     1741 tccggtaatg acgtttctga tccggacacc ggtcaccacg tcttgtccaa gcacgtcttc
     1801 gacgacgctg ttccagatga atgcaatttt ttcatttttc atggcccggt cttgcatgat
     1861 tttggaagcc cgaagtttgt cacgacgatg aattatcgac actttggtgg cgaacttggt
     1921 gagaaacgtc gcttcctcca tcgcactgtc cccgccgcct acgaccacga gctctttgcc
     1981 tttaaagaag aacccatcgc aggtcgcgca gctcgaaaca ccatagcccc ataatcgttt
     2041 ttcgtttgtg agaccaagag tgatcggaga ggcgccggtc gcaatgatca atgtcttggt
     2101 ttcaaaggtt tcttcaccat ccacaatgac tttaaaaggc cgagtttgga gatcggccga
     2161 tgtggcgtca ccggttttga agaccgttcc gaaccgctct gcttgagatc gcatctcttt
     2221 cattaactcc ggccccatga tgccctttgc gaaaccaggg tagttttcga cttccgtggt
     2281 cgtcgtgagc tgcccgcccc cttgccagcc ttcgacgagg aggggggcga gattcgcacg
     2341 agcagcatag atggctgcgg tgagtccggc tggaccggag ccaatgatga caacattacg
     2401 catgttggga ctttatcaca gtgcgggaag gagacggtag gagctacttg ggatcggccg
     2461 aaatcaagac gtaatgcaat tcgctctgca gtcccaaccg ttccgcccaa gaccagagat
     2521 agtcgaaatc gagctggaga ttggggttga gcatgatact gatcgcatct tcaaaatcgt
     2581 ggccacggct tgccttgagc ttcattaaga tgaggtcttc aggtccacac acccaagtct
     2641 gtagccccac gatcatgaaa ggccttcgcc ttcgcaaagt ttcctcttca tgtcgatctg
     2701 cgacaaagat aacgtcgaga ggaatgtcat gaaacgatgt gtgaaacaat cggttgactc
     2761 gatcagctgc aaatggattg tggtcaagcc aattctgatc tatgtgaaac cccttttcgc
     2821 ggagacacgc catcaatcgt gctcgctgca attcctcagc caccatgaga aggtcgatat
     2881 ctttggtgga acgaatccgg ccccacgcac ccagcgccaa ggcacccacc aggcaataga
     2941 tcgggggagg cgtgggcaaa gcatctaaga tggtacggag gaagttctct agagattctc
     3001 gctcaggcgg atgtgtcatt gtgatcgtgg aggaatcgag caggaggttc gaaaggagga
     3061 ggtaccggca gcggggcatg gcccaaggcc tgtttcattg ctcgttcaag cacacgatga
     3121 gcgtcagaca gccggcagac ttgcagaatt ccctcttccg gtgtggccga atagtcactc
     3181 ggactgagct gtgccgcgct cacatgcaac ttgcgcaacc agatcggcgg gtcatcggga
     3241 tcaatcggca tggaggtatg atagcgcaga tacagaaaaa agcctatcgg ggagtggcca
     3301 tggacggtta cttgatcgat ggaggttctt tgccttcttc tacctcagcc aggtaatcga
     3361 ccatcttgtc gatagcgagg gcagtaagtt tgctg
//
LOCUS       DATMTI010000025         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_722316, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000025 DATMTI010000000
VERSION     DATMTI010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722316"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VN039_00440"
     CDS             complement(<1..668)
                     /locus_tag="VN039_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44489.1"
                     /translation="MLSIPAAATAALEQSGGRSFSLQAFYGAEQTLSDVPVTFDGSLQ
                     FTSTGQIQASGTVSLARDGGDSLVPQAKTDHLAPYGQELAINYVVSFGGDSWTIPLGR
                     FRIKAVPKAKNYFRRWPDQAKVVGWAAQLSLVDRFDIIEAADFLAVTAPVPGNSTWDE
                     IQRLSPIPIVKNLDDQTLPSGMVYQSRSDAITQLFTNLGADPALTRQGALVGRVKDPW
                     LTET"
     gene            complement(669..>1511)
                     /locus_tag="VN039_00445"
     CDS             complement(669..>1511)
                     /locus_tag="VN039_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44490.1"
                     /translation="GSGIYEFDTRAGGPILTVTNIPPSVHIVFESADLPVGTHTINLF
                     RTILGVETPVREGVGAFADGGWVGDDWEVPPGVTVSYRAEGFDIDGVSLGFTVSQSCD
                     IGFEGAGVGWISDPLDSTSAIRVVMAKSAGQSPSRPMQGNLYQVGLRTVALVSVQSLV
                     TGLNMDFFTYTEADRTAVQTLLAHTGGLILIRTPPPMMVPRQLYCWASNASPAEYNLG
                     GGVQKVRWSNQVSEISAPEGNLFVTTVTYQTYIDAFPTATYGDVMAVYTSTYFDAMKN
                     PPGA"
BASE COUNT          404 a          495 c          344 g          268 t
ORIGIN      
        1 tcagtttccg tcaaccacgg gtctttcacc cgcccgacga gtgcaccttg gcgtgtcagt
       61 gccgggtccg cgccgaggtt ggtgaacagt tgtgtgatcg catccgacct cgactggtac
      121 accatcccgg aaggtaaagt ctggtcgtcc aggttcttca caatggggat cggtgacagg
      181 cgttgaatct catcccacgt actgttaccg ggaacgggtg cggttactgc aaggaaatcg
      241 gcggcctcaa taatgtcaaa ccggtccacc aacgacaatt gcgcagccca accaacaacc
      301 ttcgcctgat ccggccaacg acgaaaatag ttcttcgcct tcggaaccgc ttttatacga
      361 aaacgaccca acgggatcgt ccacgaatcc ccaccaaacg acaccacata gttgatcgcc
      421 aactcttgcc catacggtgc aagatggtcc gttttcgctt gcggaaccaa actgtcgcca
      481 ccatcacgcg ccaacgaaac agtgcccgac gcctgaattt gcccagtaga cgtaaactgc
      541 aacgacccat caaacgtgac aggcacatcc gacaacgttt gctcagcacc ataaaacgct
      601 tgcaaagaaa aactgcgacc ccccgactgt tcgagagccg cagtagcagc agcaggaatg
      661 ctcaacattt acgcccccgg aggattcttc atcgcatcaa aataggtgga cgtatacacg
      721 gccataacgt cgccgtacgt ggctgtcggg aacgcgtcaa tgtacgtctg atacgtcacc
      781 gtcgtcacaa acaggttccc ctcaggtgcg ctgatctcgg acacctgatt ggaccaacga
      841 accttctgca caccaccccc gaggttgtac tccgctggag acgcattcga cgcccagcaa
      901 tacaactgac gtggcaccat cattggcggg ggtgtgcgaa tcagaatcag cccacccgta
      961 tgcgccaaca acgtttgaac cgcagtccgg tccgcctccg tgtacgtgaa aaagtccata
     1021 ttcagaccag tcaccaacga ctgcacagac accaacgcaa cagtcctgag cccaacctga
     1081 tacagattcc cctgcatcgg acgagacggc gactggcctg cggacttcgc catcacaaca
     1141 cggatagcag aagtcgaatc caacgggtca gaaatccacc caaccccggc accctcaaac
     1201 ccgatatcgc acgactgtga caccgtaaac ccaagactca caccgtcaat gtcgaaccct
     1261 tcagcccgat acgacacggt cacacccggt ggaacctccc agtcgtcacc aacccaacca
     1321 ccatcagcaa acgcacccac cccttcacgg acaggcgttt caacacccaa aatggtgcga
     1381 aacaaattga ttgtgtgcgt tcccacaggc agatccgcag actcgaaaac aatgtgcaca
     1441 gacggcggaa tattcgtcac cgtcagaatc ggcccaccag cacgagtgtc aaactcataa
     1501 ataccggaac c
//
LOCUS       DATMTI010000026         1667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_340445, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000026 DATMTI010000000
VERSION     DATMTI010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340445"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VN039_00450"
     CDS             <1..316
                     /locus_tag="VN039_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWV44491.1"
                     /translation="HPIDQMIGQPVFRIFQRRETGVNSLAQVYRTARLKGGATYEAVC
                     HRRNGSSCHLRVVVKPLWNQFGRLHGYSLVLQKSVTVQSADGEPDNTHEEESSPLNPT
                     DN"
     gene            complement(457..1173)
                     /locus_tag="VN039_00455"
     CDS             complement(457..1173)
                     /locus_tag="VN039_00455"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44492.1"
                     /translation="MSDLWRDTMVAGFRDAVMQILLVLPRLLALVTFLLVGLAMAWLV
                     RALTVRVLAAFRFDRLCERFGLNQPLAQAGIKQPASVVLSRILFWTVFLLFAFMGVDA
                     VNLPATANLISQIVGFLPNVLAAALVLLIGVLSANFFAEAALIAAVNAQLQEARIVAN
                     LVRWGILLFTFAMVLTQMGIAKEIVISAFSITFGGVVFSLALGVGLGIRHLMREAIER
                     RLGKGKEREKEEKIDELSHL"
     gene            complement(1166..>1667)
                     /locus_tag="VN039_00460"
     CDS             complement(1166..>1667)
                     /locus_tag="VN039_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HWV44493.1"
                     /translation="AAVGAIFHSMENYEPAWERTHESTDVPLYGVVEYAPLAGPVNIA
                     RMVSGVRQGMRDLLPLWEVILSPETLGQILTLGIQESEEFHFPENLWVQVVYEFALAY
                     HDQVLHREHLLKSLTPLYLGQTASFVLDTQRGGTEQVEASVESLCRRFEAMKPYLTDR
                     WRWRDE"
BASE COUNT          401 a          520 c          432 g          314 t
ORIGIN      
        1 tcatccgatc gaccaaatga tcggacaacc tgttttccgc attttccagc gccgagaaac
       61 cggggtgaac agcttggcgc aggtctaccg aacagctcgt ttgaaaggag gcgcaacgta
      121 tgaggcggtg tgtcaccgtc gcaatggttc ctcgtgccat ctacgcgtag tcgttaaacc
      181 gctttggaat cagttcggac gcctacacgg atattctctt gtcttgcaga aatctgtcac
      241 tgtgcagagc gccgatggag agcccgacaa cacgcatgaa gaagagtcgt cgccattgaa
      301 ccccaccgat aactgagtgc cactctgaag ccgagttgca ggacatccgc acctccgtgg
      361 gctcaaagcc gggcttgcta cgctgtgacg ggagctggtt ttttagatat tcaacagatc
      421 atcaaatcgc cagatttctc acttcatgct ccatgctcac agatgcgaca actcgtcgat
      481 cttctcttcc ttttcccttt cctttccctt tcccaggcgc cgttcgatgg cctcacgcat
      541 cagatgccgt attccgagac ccacgccaag tgcgagcgag aagacgacgc cgccgaacgt
      601 aatagaaaac gccgagatga cgatctcttt ggcgatgccc atctgcgtca gcaccatggc
      661 aaacgtgaat aagagaatcc cccaccgaac cagattcgcc acgatgcgcg cctcttgcag
      721 ttgcgcattc accgccgcga tcagcgccgc ctccgcgaag aaatttgcgg acaatacgcc
      781 gatcagcaat accaacgcgg ccgcaagcac gttcgggaga aagcccacaa tctggctgat
      841 gagattggcc gtggccggca gattcaccgc gtccacgccc atgaacgcaa agagcaaaaa
      901 caccgtccaa aacaagatgc ggctcaggac gaccgacgcc ggttgtttga tgcctgcttg
      961 agccagcggc tgattgagcc cgaaccgctc gcaaagacga tcgaagcgaa aggccgcgag
     1021 gacgcgcacc gtcagcgctc tgacaagcca cgccatggcc aatccgacca aaaggaacgt
     1081 gacgagcgca agaagtctcg gcaataccaa caggatctgc atcacggcgt cacgaaatcc
     1141 tgctaccatt gtgtcgcgcc agaggtcact catcccgcca cctccatcga tccgtaagat
     1201 aaggcttcat cgcctcgaac cgccggcaga gcgactctac ggacgcctcc acctgctcgg
     1261 tgccgccgcg ctgcgtgtca aggacaaaag aagcggtctg cccgagatac aatggggtca
     1321 gcgatttcag caaatgttcc cggtgcagca cttggtcatg ataagccagt gcaaactcgt
     1381 agacgacctg cacccaaaga ttctcgggaa aatgaaattc ctcggactcc tggattccga
     1441 gcgtgaggat ttggcccagt gtctccggcg agaggatgac ttcccacaac ggcaacagat
     1501 cgcgcatccc ctgccgcact ccactgacca tgcgggcgat gttgactggg ccggcgagcg
     1561 gcgcatattc gaccacccca taaagcggca catcggtcga ctcatgggtg cgttcccagg
     1621 ccggctcata attctccatc gaatgaaaga tcgccccgac ggcagcc
//
LOCUS       DATMTI010000027         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_298055, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000027 DATMTI010000000
VERSION     DATMTI010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298055"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..884
                     /locus_tag="VN039_00465"
     CDS             339..884
                     /locus_tag="VN039_00465"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HWV44494.1"
                     /translation="MSELVCIAFKDSSTADRVLNELRAMETEYVLDLEDAVIVVRDMD
                     GKVHLKQCVDVFGGATKHGVALGVLWGGLMGLLFMNPLAGLLGSIAGGASGGAMTTAA
                     SEYGLLSDYGIPDNFIKDLGSTIKRGTSAIFLLIRSVDEDKVLSRISRYEGTILKTSL
                     SKEHEEKLRVALTHQRNQKKV"
     gene            complement(885..2141)
                     /locus_tag="VN039_00470"
     CDS             complement(885..2141)
                     /locus_tag="VN039_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HWV44495.1"
                     /translation="MDSQVLEEATTTTSRTGLIARWQNVPLYARIVIALVLGVMAGLM
                     LGSEAAVLAVPGKLVLRLLGALAPPLILAAIVHTFMTTHLGGPLAMRLPRLLLLNTFV
                     AIFVGLTVANIIQPGHGAGLTPPAPHEEATKAANPLVTFLENVPKSLLGPLSDDGKVI
                     GVIFIAVAFGMALRKERERPLGTVGHLVELFLESLIKILHWIIAVVPFAVFGIVASIV
                     GTEGFAPFKALGIFVLCVLLALAIQAGYYLIRIRFGSWVRPGELLHGGRDALVMAFST
                     ASSTATMPVTYAALKDRVGLREQSASMGALVGANFNNDGTALYEAMAALFIAQMIGMD
                     LTVQQQVMVVLTSIIASVGAAGIPEAGLVTMTMVFTAVGLPVQYIPVLLTVDWFLDRC
                     RTAINVMGDMNVSCLLDGKQKGGTSP"
     gene            2453..>2707
                     /locus_tag="VN039_00475"
     CDS             2453..>2707
                     /locus_tag="VN039_00475"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWV44496.1"
                     /translation="MTSALLYDRLQFAFTATFHYLFPQLTMGLALLLFYLRSRALVTD
                     DEQYQQVSDFWTSIFALNFGFGVVTGIPLEFQFGTNWARFS"
BASE COUNT          641 a          760 c          732 g          574 t
ORIGIN      
        1 gagcaaactg gtctttccgc ttccgttttc ccctaccaca gccagcagtg atccccgctc
       61 gaccttcacg ttgaggtcag cgaacaatcg tcgttcaccg cgactgcaac gtaatgaaac
      121 cgcttgcaac ataaggaact ccacgccggt gcgcgacgag agtaagggaa cagagcgtgg
      181 aaaaacaagg ggcgaggata tgcgttgcca tgacccggtt cagagcccat cttgctctga
      241 gagcaatcct actgttatct agtgcatgcg ccacaaggtt gaccatatgt ttcaataaat
      301 gatgacgcct actcacgtac cactgaagga gatgaatcat gagtgaactg gtctgcatcg
      361 cgtttaagga ctccagcaca gccgatcggg tactgaatga actacgagcc atggagacgg
      421 aatatgtctt ggatctcgaa gatgcagtca tcgtcgtccg tgacatggat ggcaaggtgc
      481 acttgaagca gtgtgtcgat gtgttcggtg gagcaaccaa gcatggtgtc gcgctgggcg
      541 tgttatgggg agggctcatg ggactgcttt tcatgaatcc tctggcgggt ctattgggca
      601 gcattgccgg aggagcaagc ggaggagcca tgaccaccgc cgccagcgag tatgggctat
      661 tgagcgacta tgggatcccc gacaatttca tcaaggactt gggaagcacg atcaagcgag
      721 gcacttccgc gatcttcctg ttgattcgca gtgttgacga agacaaggtg ttatccagga
      781 tttcacggta cgaaggaacg atcctcaaga cctcactcag caaggagcat gaagagaaat
      841 tgcgagtggc cctcacacac caacgaaatc aaaagaaggt atagtcatgg actggttcct
      901 cctttctgtt ttccgtccaa gagacaactc acgttcatat cgcccatgac gttgatcgcg
      961 gtacggcagc gatcgagaaa ccaatcgacg gtcaacaaga cagggatgta ttgcacgggc
     1021 agtcctacgg cagtaaagac catggtcatc gtcaccagac cggcttcagg gatgccggcg
     1081 gcgccgacgg atgcaatgat gctcgtgagc accaccatca cttgctgctg aacagtcaga
     1141 tccatcccga tcatttgcgc gatgaataag gccgccatcg cctcgtacag cgcggtgcca
     1201 tcattgttga aattggcacc caccagcgct cccatactgg cggactgctc gcgcaatccc
     1261 acacgatctt tgagcgcggc gtatgtcacc ggcatcgtcg ccgtcgagct ggctgtcgag
     1321 aaggccatca ccaatgcgtc acgtccgcca tgcagcaatt cacctgggcg cacccacgac
     1381 ccgaagcgga tccgaatgag gtaataccct gcctgaatcg ccagcgcgag cagtacgcat
     1441 agcacaaaaa tccccagtgc cttgaacgga gcaaacccct ccgtccctac gatgctcgcg
     1501 acaatgccga aaacggcaaa cggcaccact gcgatgatcc agtgcaaaat cttgatcaac
     1561 gattccagga acagctcaac gaggtgtcca accgttccaa gaggccgttc acgttccttg
     1621 cgcaaggcca tcccaaaggc tacagcaatg aaaatcacgc cgatcacctt cccatcgtca
     1681 ctgagcggcc ccagcagact cttgggcaca ttctcaagaa aggtgacaag aggattggcc
     1741 gccttcgtcg cttcctcatg cggagcaggc ggcgtgaggc ccgcgccgtg ccctggttga
     1801 atgatgttgg caacggtcag gccgacgaag atcgcgacga acgtattcag taacaacaat
     1861 cgcggcagcc gcatcgcaag cggcccaccg aggtgcgtcg tcatgaacgt gtgcacgatt
     1921 gccgccagaa tcagcggcgg cgcaagcgca ccgaggaggc gcaacacgag cttgcccggc
     1981 actgccaaca cagccgcttc gcttcccagc atcaaccccg ccatgacgcc gagtaccagc
     2041 gcaatcacaa tgcgcgcata gagcgggacg ttctgccacc gtgcaatcag gccggttctc
     2101 gatgtggtgg tcgtcgcttc ttccaaaacc tggctatcca cttaatggaa ttcctattcc
     2161 tccactcatt gtgctgagtg agaagagatc gccgaatgta ccacgaagag ggtgactgat
     2221 gagccaagga atgtgtgagg agatggctgt cttctgtgac tcagtcggcg tgctcgttga
     2281 tggcatacgc gaggatcagt ggttctcgtg tcacgcatct tgtatggtac cgaatcggtc
     2341 acacaaacga ctcgcggtgc tttttcagtt tgggatatct cggttctagt tgttaaagtg
     2401 ctggatggcg cgagacctgt tcttccttgt tcataggacc tgagaaaata tcatgacaag
     2461 cgcactactg tacgaccgtc tccagtttgc attcaccgcc acgttccact acctctttcc
     2521 gcagttgacg atgggtctgg cgttgctgct gttctacttg cgtagccggg cgcttgtcac
     2581 cgatgacgaa cagtatcagc aagtctcgga tttttggacg agcatctttg ccttaaattt
     2641 cggcttcggc gtcgtgaccg gcatcccgct cgaatttcag tttggaacca actgggcgag
     2701 gttttct
//
LOCUS       DATMTI010000028         1949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_170888, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000028 DATMTI010000000
VERSION     DATMTI010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170888"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1937
                     /locus_tag="VN039_00480"
     CDS             132..1937
                     /locus_tag="VN039_00480"
                     /inference="COORDINATES: protein motif:HMM:NF039819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HWV44497.1"
                     /translation="MVIINSAEANRAQLRYIKEAVWGVTPANGNTAEARITDSSLTRT
                     KNTQTSKEIRADRMVPSIAEVGSSAGGSVDFEFSAGSLDAFLEGFLLGAWTRSMNFFQ
                     VKGNTVSVTATDTVKILGVDYTNYITANQYLKLEGFTDPANNKYIKVTSVAFTAGATV
                     ITATGAALVAEAGTAFTKVLDANDVILRATDIEIAAGNEITTTAGSFAGLKVGQTIYF
                     EGLGKGEGSVLWNAADPVAGDTITVSDGTNTVVFEINTSPDAVGEGNVYVALSDTEAT
                     MGQNFTDAVNAQWAKGVLKVTSEFATATSTLTNHAGAGGSIVASDVTTATVTNFAGGD
                     NAKFGFLTVLALPDSKTIQVAETLTVDTNASALPAIVKGSHLRNPGVVSEITKQSFTV
                     ETSFTDVSKNFVARGQRPGSFSLDVKTGDIVTGSIDFMGRDIVHSSTALLSNSPYVPL
                     DSTATEILNATSNVGSIKYQGTPLDLAIQNITMKGDNNLREQRAVGEQFPAGIGYGKF
                     DLSGTADAYFESFELYQAFVDHTTVSLSFDFTDVDNNHYIFTVPAVKFTKDPISPDGV
                     DTDVMEKMEWTAQRDPVLKTQLMVDRYSSIWPMAA"
BASE COUNT          440 a          537 c          540 g          432 t
ORIGIN      
        1 agccagccga cgcttagggc aaggttttcc ttgactattt cctaaaacta tggttaacga
       61 taaggtgacg aattgggaat gctcgcgccg tgggaggcgc gggtataacg cgtcttaggg
      121 ggcttccttt catggttatc atcaattcgg ccgaagcaaa ccgggcacaa cttcggtaca
      181 tcaaagaggc tgtctggggc gtgacgcccg ccaatggtaa taccgcagag gcccgaatta
      241 ccgattcgag cttgacgcgt acgaagaaca ctcagacctc gaaagaaatc cgcgccgacc
      301 gcatggttcc gtccatcgcc gaagtcggct cttcggctgg cggctccgtc gactttgagt
      361 tctcggcggg atcactcgac gcgttccttg aaggattcct gttgggcgcc tggacgcgtt
      421 cgatgaattt cttccaagtc aagggtaaca ccgtatctgt tacggcgacg gacaccgtaa
      481 aaattcttgg cgttgactac acgaactata ttaccgccaa tcagtatttg aagctcgaag
      541 ggttcacgga cccggcgaac aacaaataca tcaaagtaac ttctgtcgct tttacggcgg
      601 gtgcgactgt cataacggca acgggcgccg cgctcgtggc cgaggccggt acagctttca
      661 cgaaggttct tgacgccaac gacgtcatcc tccgtgcgac ggatattgaa atcgccgcgg
      721 gcaacgaaat caccacgact gccggatcgt ttgccggact gaaagtcggc cagacgattt
      781 atttcgaggg cttgggcaag ggtgagggta gcgttctttg gaacgccgct gatcccgtcg
      841 cgggcgatac gattacggtt tccgatggca cgaacacggt cgtgttcgaa atcaatactt
      901 cgcctgacgc cgtcggcgag ggcaacgtct atgtcgcgtt gtctgatacc gaagccacga
      961 tggggcagaa tttcaccgac gccgtaaacg cccaatgggc taagggcgtg ctgaaagtca
     1021 cgtcggaatt cgccacggca acttctacac tcacaaacca cgcgggtgcg ggcggctcta
     1081 tcgttgcatc cgacgtcacg actgcgactg tcacgaattt tgcgggcggc gacaatgcta
     1141 agttcgggtt cctgactgtc ttggcactcc cggatagcaa gacgattcag gttgccgaga
     1201 cactgactgt cgacacaaac gcctctgcgt tacctgcgat cgtgaaaggt tcgcatttgc
     1261 gcaaccccgg cgttgtgtct gaaatcacca agcagtcatt tacagttgag acgtcgttca
     1321 ctgacgtgtc gaagaacttt gttgcacgcg ggcagcggcc gggatcgttc agtctcgacg
     1381 tcaagacggg agacattgtg accggctcta tcgactttat gggccgtgac attgtccact
     1441 cgtcgacggc gcttttgtct aactcaccgt atgtgccgct agattctacc gcgacggaaa
     1501 ttctgaacgc aacgtctaac gtcggcagca tcaaatatca aggcacgccg cttgatcttg
     1561 caattcagaa tatcactatg aagggcgaca acaacctccg ggaacaacgc gccgtcggcg
     1621 aacagttccc ggccggtatc ggctacggaa aattcgacct ttccggaact gccgacgcgt
     1681 actttgaatc gttcgagctg tatcaggcgt ttgtcgacca cacgacggta tcgttgtcgt
     1741 tcgactttac cgacgtcgac aacaaccact acatctttac ggtccctgca gtgaagttca
     1801 cgaaggaccc gatttctccg gatggcgtcg acacggacgt tatggagaag atggagtgga
     1861 ccgcgcagcg cgatccagtc cttaaaacgc agctcatggt tgacaggtat tcgtccatct
     1921 ggccgatggc cgcgtaagtc gactacgcc
//
LOCUS       DATMTI010000029         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_764677, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000029 DATMTI010000000
VERSION     DATMTI010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764677"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..641
                     /gene="rpsJ"
                     /locus_tag="VN039_00485"
     CDS             333..641
                     /gene="rpsJ"
                     /locus_tag="VN039_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWV44498.1"
                     /translation="MNGQNIRIRLKAFDHRILDASTREIVSTAKRTGASVRGPVPLPT
                     RIEKFTVNRSPHVDKKSREQFEMRTHKRLLDIVDPTPQTVDALMKLDLAAGVDVEIKL
                     "
     gene            846..1487
                     /gene="rplC"
                     /locus_tag="VN039_00490"
     CDS             846..1487
                     /gene="rplC"
                     /locus_tag="VN039_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWV44499.1"
                     /translation="MRSGVIAQKVGMTRVYNDAGEHIPVTVLRLDNVQVVAQRTEDKN
                     GYTAVQLGAGQSKVKNTTKALRGHFAAANVEPKAKLVEFRVSPENLIDIGATLTANHF
                     QSGQLVDVTGTTIGKGFAGAMKRHNFGGGRASHGNSVSHRAHGSTGNNQDPGRVWKGK
                     RMAGHMGQTRVTTQNLEVVSTDEDRGLILVKGAVPGSKGSWIIVRDAVKSAAK"
     gene            1500..2120
                     /gene="rplD"
                     /locus_tag="VN039_00495"
     CDS             1500..2120
                     /gene="rplD"
                     /locus_tag="VN039_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWV44500.1"
                     /translation="MELNVKTLEGKDAGKVSLSDEIFGLDPRQDILARMVRWQLAKKQ
                     QGTHKSKNRSEVSRTGAKMYKQKGTGRARHHSARAPQFRGGGKAHGPVVRSHAHDLPK
                     KVRALALRHALSAKLKAEELIIVDQLVASEAKTKALLGSFASLGLTNALVIGGAELDG
                     NFKLAAQNIPNVDVLPVQGINVYDILRRGKLVLSKAAVEALEERFK"
     gene            2117..2410
                     /locus_tag="VN039_00500"
     CDS             2117..2410
                     /locus_tag="VN039_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWV44501.1"
                     /translation="MTDLRHYDVIVSPSITEKSTLVSEQNQVVFNVAKNASKPEIKAA
                     VEALFGVKVTAVNTLIRKGKTRRFRGFAGKLKDVKKAVVTLAEGQSIDVSTGL"
     gene            2454..3290
                     /gene="rplB"
                     /locus_tag="VN039_00505"
     CDS             2454..3290
                     /gene="rplB"
                     /locus_tag="VN039_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWV44502.1"
                     /translation="MALKSFNPTTPSQRQLVIVDRASLYKGKPVKALTQGLSSKGGRN
                     NQGRITVRFQGGGHKRTYRLVDFKRRKFDVEGTVERLEYDPNRTAFIALVTYTDGEQA
                     YILAPQRLAAGDKVIASDKAVDVKPGNTMPLQYIPVGSIIHNVEMKPGKGGQIARSAG
                     TYVQLVGRDAGMAILRLNSGEQRLVHGSCLASIGAVSNSDHGNINDGKAGRSRWRGKR
                     PHVRGVVMNPVDHPHGGGEGRTSGGRHPVTPWGKPTKGKRTRSNKSTDKFIMRSRHQR
                     KK"
     gene            3306..3584
                     /gene="rpsS"
                     /locus_tag="VN039_00510"
     CDS             3306..3584
                     /gene="rpsS"
                     /locus_tag="VN039_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWV44503.1"
                     /translation="MARSVWKGPFVDGYLLTKAEKVRESGRNEVIKIWSRRSTILPQF
                     VGLTFGVYNGSKHVPVSVNEDMVGHKFGEFSPTRTYYGHGADKKAKRK"
BASE COUNT          862 a         1091 c         1056 g          717 t
ORIGIN      
        1 gttgcatcga tcgtcgagta atttcgacca ttgaaattct gactggcggc ggtgtgaaca
       61 acaccgccgc cggcctttta gtaaaaagcc tggcggcgtt gcatttttag tgcttgaaaa
      121 attcggtgaa agcgcgtaaa cgcgcactca caatcaccgc tggattcgca agcgattgcg
      181 gaacgcggtg tttttagcaa tacagactac cgaaatgatt ggggtgcaac ccgcatccct
      241 ctcgattttt gaaaatctgc ggcgccacga tcaatagaag ttcatgtgtt tccgcgtgcg
      301 gaaacgaata acaaagaaca aggacaagac gaatgaacgg ccagaatatc cgtatccgcc
      361 tcaaggcgtt cgatcaccgg atcctcgatg cctctacccg tgaaatcgtg tcgaccgcaa
      421 agcgcaccgg cgccagcgtt cgcggcccgg ttccgctccc cacccgcatc gaaaagttta
      481 ccgtcaaccg ctctccccac gttgataaga agagccgtga acagttcgaa atgcggacgc
      541 acaagcgtct tctcgatatc gttgatccca ccccgcagac tgtcgatgct ctgatgaagc
      601 tcgacctcgc tgccggcgtt gacgtggaaa tcaagctcta agacccggcc cgaccccatc
      661 cagggtgagg gctgaagtaa aaggccggac ttcacctttg ggtgagggcc aataacaagg
      721 aaggtacgtg gagacatcca acgacttcca aaccggagac cggaaacatc gtgagatgtc
      781 gaagggcact ccttaacaaa ggccagagag ggttttccct tcaaggctcg caagaggatg
      841 aaccaatgcg ttcaggtgtg attgcacaga aagtgggtat gacacgcgtc tataacgacg
      901 caggtgaaca tatccctgta acagtattgc gactggataa cgtacaagtc gttgcccagc
      961 gcacggaaga caagaatggc tacaccgcag ttcagctcgg tgccggccag tccaaggtca
     1021 agaacacgac gaaggcgctt cgcggtcatt tcgccgctgc caacgtcgag ccgaaagcca
     1081 agctcgtcga gttccgggtt tcccccgaga acctgatcga catcggtgca acactgaccg
     1141 caaatcattt tcagtccggc cagctggtcg acgtcactgg aaccacgatc ggtaaaggtt
     1201 ttgccggtgc tatgaagcgt cacaacttcg gtggtggccg cgcttcgcac ggtaactccg
     1261 tatcgcaccg tgcacacggt tcgaccggta acaaccagga tccgggccgc gtctggaagg
     1321 gcaagcgcat ggctggtcat atgggccaga cgcgcgttac gacccagaac ctcgaagtcg
     1381 tttcgaccga tgaagaccgt ggcctcatcc tcgtgaaggg tgcagttccc ggctcgaagg
     1441 gttcctggat catcgtccgc gacgccgtca agtcggctgc gaagtaaggg agccttatca
     1501 tggaattgaa cgtcaaaacc ctcgagggaa aagacgccgg caaggtttct ctgtcggacg
     1561 agatcttcgg cctcgacccc cgccaggaca tcctggcccg catggttcgc tggcagcttg
     1621 caaagaagca gcagggaacc cacaagtcca agaaccggtc ggaagtttcc cgcaccggcg
     1681 cgaagatgta caagcagaag ggtacgggcc gcgctcgtca ccattcggct cgcgcaccgc
     1741 agttccgcgg cggcggcaag gcccacggcc cggtcgttcg cagccacgcc catgaccttc
     1801 ccaagaaggt tcgtgcgctt gctctgcgtc acgccctgtc tgcaaagctg aaggctgaag
     1861 aactgatcat cgtcgaccag ctcgtcgcgt ccgaagcaaa gaccaaggct ctgctgggca
     1921 gcttcgcttc gcttggcctg accaacgctc tcgttatcgg cggcgccgaa cttgatggca
     1981 acttcaagct cgctgctcag aacatcccga acgtggacgt tctgccggta cagggcatca
     2041 atgtttacga catcctgcgc cgtggcaagc tggtgctttc caaggctgct gtagaggctc
     2101 tggaggagcg gttcaaatga cggatcttcg ccactacgat gtgatcgtat ctccttcgat
     2161 cacggaaaag tcgacgctgg tatccgaaca gaaccaggtc gtattcaacg tcgccaagaa
     2221 tgcttccaag cctgaaatca aggctgccgt ggaagctctc ttcggcgtca aagtcacggc
     2281 tgtgaacacg cttatccgca agggtaagac ccgtcgtttc cgtgggttcg ccggtaagct
     2341 gaaggacgtc aagaaagccg tcgttacgct cgccgaaggt caatccatcg acgtgtccac
     2401 cggactctaa aggttaggcc caagcgggca aagacccaaa agggaacaag aatatggcat
     2461 tgaaaagttt caatccgacc acgccgagcc agcgtcagct ggttatcgtg gaccgcgctt
     2521 cgctctacaa gggcaagccg gtaaaggctc tcacccaggg cctcagctcc aagggtggcc
     2581 gtaacaacca gggccggatc accgtccgtt tccagggtgg cggtcacaag cggacctacc
     2641 gtctggttga cttcaagcgt cgcaagttcg acgttgaagg caccgttgaa cgtctggaat
     2701 acgaccccaa ccgcaccgcg ttcatcgcgc tggttacgta taccgacggc gaacaggctt
     2761 acattctcgc gccacagcgt ctcgctgccg gtgacaaggt gatcgcctcc gacaaggcag
     2821 tggacgtgaa gcccggcaac accatgccgc ttcagtacat tccggttggt tcgatcatcc
     2881 acaacgtcga gatgaagccg ggtaaaggcg gtcagattgc ccgctccgcc ggtacgtatg
     2941 tccagctcgt cggccgcgat gccggtatgg ctatcctgcg tctcaactcg ggcgaacagc
     3001 gtctggtgca cggctcctgc ctcgcatcga tcggcgctgt ttcgaactcg gatcacggca
     3061 acatcaacga cggtaaagcc ggtcgttcgc gttggcgtgg caagcgtccg cacgttcgcg
     3121 gcgtcgtcat gaacccggtc gaccacccgc acggtggtgg tgaaggtcgc acgtcgggtg
     3181 gtcgccaccc ggttactccg tggggcaagc ccacgaaggg caagcgcacc cgttcgaaca
     3241 agtcgaccga caagttcatc atgcgctcgc gccatcagcg taagaagtaa gagaggtagt
     3301 cactaatggc tcgttcagta tggaaaggtc cgtttgttga cggctatctt ctcaccaagg
     3361 ctgagaaggt gcgcgagagc ggacgtaacg aagtaatcaa gatctggagc cgtcgctcca
     3421 cgatcctgcc gcagttcgtt ggtctgacgt tcggcgtcta caacggcagc aagcatgtgc
     3481 ccgtctctgt caacgaagac atggtcggac acaagttcgg tgaattctct ccgacccgta
     3541 cctattacgg tcacggcgcg gacaagaagg caaagaggaa gtaatcatgg cgaaggctaa
     3601 gaccgaacgc cggctgaagg acaatgaggc tcaggccatt gcccgtacgc tccgcgtcag
     3661 cccccagaaa ctgaacctgg ttgccgctct tatccgtggc aagaaggttg atcgcgctct
     3721 cgccga
//
LOCUS       DATMTI010000030         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_637544, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000030 DATMTI010000000
VERSION     DATMTI010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637544"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..787)
                     /gene="narI"
                     /locus_tag="VN039_00515"
     CDS             complement(95..787)
                     /gene="narI"
                     /locus_tag="VN039_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HWV44504.1"
                     /translation="MSNLHEFFFQIYPYIAGSVFLLGSLLRFERGQYTWTSDSSELLR
                     RGTLRFGSNLFHVGVLFLFMGHFIGILMPEVFGIIGIGLQGHQLVAMISGGFFGFACF
                     VGLVMLLHRRMTEPRIRATTRVMDMVVLVWILITLSFGLCTLYFSAQELSGGNLIPLS
                     QWARHIITFHGGAASFVVALPMVYQVHMVLGMTLFALIPFSRLVHIWSGFALVVYLLR
                     PYQVVRPGLRQR"
     gene            complement(815..1453)
                     /gene="narJ"
                     /locus_tag="VN039_00520"
     CDS             complement(815..1453)
                     /gene="narJ"
                     /locus_tag="VN039_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HWV44505.1"
                     /translation="MRVYKVIALLLTYPEQDWVQSIGELRAMESAETGRGRRARHRLD
                     RLLIYLERGQLIDLQEAYVETFDRNPAHSLSIFEHTMGESRDRGSAMVSLVEEYRRSG
                     LEVTSRELPDFLPVYLEFLSLIPPEEAQGRLARIADVVQILCRRLTDLDSPYAGAFDA
                     LTALTSRPALSGTSSPIQKMKRFLRRKQSGIRMGVPGTLTKEPPGDDGPRET"
     gene            complement(1450..>2680)
                     /gene="narH"
                     /locus_tag="VN039_00525"
     CDS             complement(1450..>2680)
                     /gene="narH"
                     /locus_tag="VN039_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HWV44506.1"
                     /translation="FDYNTLQHAPEGPTVPTARPMSIITGEVKDKIDGGPNWDDDLGG
                     ESGERFKDVNVEGIDPDRLAAFENTFLFYLPRICEHCLNPTCVASCPSGSIYKREEDG
                     IVLVDQDKCRGWRMCVSGCPYKKIYYNWHTGKAEKCTFCYPRIESGQPTICSETCVGR
                     LRYLGVMLYDADRIEKAASVEQEQDLYEAQLGLFLDPKDPAVQAQARQDGISDNWLEA
                     AKRSPVYKMAVEWRLAFPLHPEYRTLPMVWYIPPLSPVSNSVDMAHVETDELPDSSSL
                     RIPVRYLAHLLTAGNEEPIFRALDRLLAMRRYMRKKDMGDDAALSLPRVGLSESQIEE
                     MYRYLAIADYEDRYVIPTNHQEAMSQSFDDQGSCGFSADSRFSEGVTGASLFAGKERS
                     NAQLISVDEIRRGNRRP"
BASE COUNT          599 a          798 c          748 g          535 t
ORIGIN      
        1 gcgggccatg accccgtggc gctgaccggt ggttcccttc gccatcgggc gaaatgcctc
       61 ctgcttcgtc agcgggacga tttcaaaact ttccttaccg ttggcgcaat cccggacgca
      121 ccacctgata cggtcgcagg aggtagacca ccaatgcaaa tccgctccag atgtgtacga
      181 gtctgctgaa cgggatgaga gcaaacagag tcatccctaa taccatatga acttgataga
      241 ccatcggcag cgccaccacg aaagaagcag cgccgccgtg aaacgtgatg atatgacggg
      301 cccattggga caatggtatc agattgcctc ctgacagctc ctgagcagaa aaatacagtg
      361 tgcacaagcc gaaggacagg gtgatgagga tccatacaag cacgaccata tccatcacgc
      421 gtgtagtcgc gcggattctc ggctccgtca tgcgccgatg gaggagcatg acgagtccga
      481 caaagcacgc gaatccgaag aacccaccgc tgatcatggc caccaactga tgcccctgca
      541 gaccgatgcc tatgatccca aacacctccg gcatgaggat gccgataaaa tgtcccatga
      601 agaggaacaa cacgccgacg tggaagaggt tactgccgaa tcggagcgtt ccgcgcctga
      661 gcaactcgct tgaatcgctg gtccaggtat actgaccccg ctcaaaacgc agcaaactgc
      721 cgaggagaaa cacggagccg gcgatgtacg gatagatttg aaagaagaac tcgtgaagat
      781 tggacatgtc acatccttat cgcctcgaat cgtgttaggt ctcacgcggt ccgtcatcgc
      841 cgggcggttc tttcgtcaac gttcccggca ctcccattcg gatcccggac tgcttccgtc
      901 gaagaaaacg cttcattttc tgaatcgggg atgaggttcc ggacaacgcg gggcgggaag
      961 tcagcgccgt cagggcatca aaagcgccgg cgtaagggga gtcaagatct gtcagccgac
     1021 gacacagaat ttgcaccaca tcggcgatac gagcgagtcg tccttgcgcc tcctccggcg
     1081 gaatcaggga caggaactcg agatacaccg gaagaaaatc gggcagctcg cgactcgtca
     1141 cctccaatcc tgaccgacgg tactcctcaa caaggctgac cattgcggag cctctgtccc
     1201 tggattctcc catggtatgt tcgaagatcg acaacgaatg ggccggattg cgatcgaagg
     1261 tttcaacgta ggcctcctgc aaatcgatca attgcccgcg ttcgagataa atcaacaacc
     1321 ggtcgagccg gtgcctcgcg cgcctgcctc gtccggtctc ggcggactcc atcgctcgca
     1381 actcgccgat cgattgcacc cagtcctgtt cgggataggt caggagcagg gcgatgacct
     1441 tgtagaccct cacggcctgc ggttccctct ccggatctca tcgacgctga tgagttgtgc
     1501 gttcgaccgc tccttaccgg cgaacaggct ggccccggtc accccctcgg aaaatcggct
     1561 gtcggcggag aagccgcagg agccctggtc atcgaagctc tgtgacatgg cctcctgatg
     1621 gttggttgga atgacgtaac ggtcttcata atcggcgatc gcaaggtacc ggtacatctc
     1681 ctcgatctgg ctctcactca gtcccactcg tggcagcgac agggctgcgt cgtctcccat
     1741 gtctttcttt cgcatgtagc gacgcatcgc aagcagccga tccaacgcgc gaaagatggg
     1801 ttcttcattt ccagccgtca ggagatgtgc gaggtagcgc accgggatcc ggagcgaaga
     1861 gctgtcggga agctcgtccg tctcaacatg cgccatatcg actgaattcg aaacaggtga
     1921 gagcggcggt atgtaccaca ccatgggcaa cgttcggtat tcgggatgga gaggaaacgc
     1981 cagacgccac tcgaccgcca tcttatagac cggggaccgc ttggctgcct ccagccaatt
     2041 gtcggaaatt ccgtcctgtc tcgcctgcgc ctgcacagcc ggatccttgg gatcgagaaa
     2101 cagcccaagt tgggcctcat acagatcctg ttcctgttcg acactcgccg ctttttcgat
     2161 gcggtccgca tcgtagagca tcacgcccag gtatcgcagg cgaccgacgc aggtctccga
     2221 acaaattgtc ggctggccgg attcgatgcg cggataacaa aaggtgcatt tctcggcttt
     2281 gcctgtgtgc cagttataat aaatcttctt atagggacag ccgctgacgc acatccgcca
     2341 gccacgacac ttatcctggt ccaccagcac aatgccgtct tcctcgcgtt tatagatcga
     2401 accggacgga caggatgcaa cgcaggtggg attcagacaa tgctcgcaaa tccgcgggag
     2461 gtagaacaga aaggtgtttt cgaatgcggc caggcgatcc gggtcaattc cctcgacatt
     2521 gacgtccttg aagcgctccc ctgactcgcc gccgagatcg tcgtcccaat tcggaccccc
     2581 gtcgatcttg tccttgacct ccccggtgat gatggacatc ggccgggcgg tgggcaccgt
     2641 cggtccttcc ggtgcgtgct ggagtgtgtt gtagtcgaag
//
LOCUS       DATMTI010000031         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_595241, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000031 DATMTI010000000
VERSION     DATMTI010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595241"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1938
                     /locus_tag="VN039_00530"
     CDS             22..1938
                     /locus_tag="VN039_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HWV44507.1"
                     /translation="MRGVATKKTNGKPRPLAMVEVLMNVVAAAGVRTCAHKFRANAGE
                     LSVGWGKSGGSEQLITLADILTRQGHRIVKVDFKNAYGTIKRRHLANAVMKHVPEYIN
                     VLRLLLGETTHVYFTDRDLVHHVEQTEGICQGSVDGPALFQLGLGEVLAGLRAKHGEI
                     IWADYLDDVFLVGDDTKALLSALKELETEAAKIGLCMNRDKTELYGRELAAREKKQAV
                     EARIKATQDGTIVLGVPVGTDEYVTKAVKAMSAETSAVANSLRSAATEARRVGLSTTK
                     GLIQHLLEVLRMCVSSKFTHIMRAIPPRLTREAAHAIDASVFVAMKTILNIDDDEVGS
                     GPEQRRVWEKASLPISGGGIGMMKLERVRTAAWVGRLLLVASDVCTTIESRHALHNNL
                     LEYATNETITTTKREPRRGEKWRRKRGKAGRTRDDDDDDEDDAPPISATSTTTSNIND
                     AADANEQRLRFLHACIPDLRYAMEEIKYYDCSGAAEEDRHIDDLSTMDLGKPLKKKQK
                     ALTAAMNASRVGNYEERYTKSSVVESVYWQSQLKGHAGRLFGLSTVSPTHRMSAEELS
                     NVGKMHLYAGGERNIETRCLLCEQTTRGDKYHALGCDVVATNQQRHAYLLRVLRTCIA
                     RQRPDLTVVESEIL"
     gene            2036..>2258
                     /locus_tag="VN039_00535"
     CDS             2036..>2258
                     /locus_tag="VN039_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44508.1"
                     /translation="MDPTIGHVFTVDVKKMEDVKVAMLHDHKMKKYRRGFKIDDERTN
                     KFLPGAMDSVGRIHEEFEDYLGSKGNDDNF"
BASE COUNT          604 a          595 c          695 g          364 t
ORIGIN      
        1 ataacggtca ccctccgccg agtgcgggga gtcgccacca agaaaaccaa cgggaagccc
       61 agaccactcg ccatggtgga ggtcctcatg aacgtcgtcg ccgccgccgg tgtacgcacg
      121 tgtgcccaca aattccgcgc gaacgctggc gaactaagcg tgggatgggg taagtcgggg
      181 gggtccgaac agctaataac cctcgccgac atcttgacgc gtcagggcca ccgtatcgtc
      241 aaggtggact tcaagaacgc gtacggcacg atcaagagac gccatctggc caatgccgtc
      301 atgaaacacg taccagaata catcaatgtc ctccgcctcc tcctcggaga gaccacccac
      361 gtctatttca ccgaccgcga cctcgtgcac cacgtcgagc aaaccgaggg gatctgccaa
      421 ggatcggtcg atgggccggc gttgtttcag ctgggactcg gcgaggtcct cgcgggactg
      481 cgggctaaac atggggaaat catatgggcc gactatctcg acgacgtctt cttggtggga
      541 gacgatacca aggccctcct ttcggcactg aaggaactcg agactgaggc ggccaagatc
      601 gggctctgca tgaaccgtga caagacggag ctttacggca gggagctcgc agcgagggag
      661 aagaagcagg cggtcgaggc gaggataaaa gccacccaag atggaaccat cgtgctgggc
      721 gtcccggtcg gcactgatga atacgtgacc aaggccgtga aggcgatgag cgctgagacg
      781 agtgcggtgg cgaactctct ccggtcggcg gcgaccgaag cccggcgcgt gggactctca
      841 accacgaaag ggctcattca gcacctcctg gaggtacttc gtatgtgcgt ttcatcgaag
      901 tttacccata ttatgcgagc aatcccccct cgattgactc gcgaagcggc ccacgcgatc
      961 gacgcgtcgg tgtttgtcgc aatgaagacc atattgaaca ttgacgatga tgaagtggga
     1021 tcaggaccag aacaaagacg cgtgtgggag aaggcgagtc tcccgattag tggaggagga
     1081 attgggatga tgaagcttga gcgagtgcga acggcggcgt gggtcggtcg actgcttctc
     1141 gtcgcgtcgg acgtgtgcac gacgatcgaa tctcgacatg cgctccacaa caatctcctc
     1201 gaatatgcga cgaatgaaac gattacaaca acaaaacgag aaccacggag aggtgagaag
     1261 tggagaagaa agagagggaa ggcgggaagg acacgcgacg acgacgacga cgacgaggac
     1321 gacgcgcccc ccattagcgc gacctcaact accacctcaa acataaacga tgcggccgac
     1381 gcgaacgaac aacgtctccg attcctacat gcgtgtatcc ccgacctcag atatgcgatg
     1441 gaggagataa agtattacga ctgctcggga gctgcggagg aagaccgtca catcgacgac
     1501 ctcagcacca tggacctcgg gaaacccctg aagaagaaac aaaaggctct gacagcggcg
     1561 atgaatgcga gtcgagtggg gaattatgag gagagataca ccaaaagctc ggtggtggag
     1621 agcgtgtatt ggcaatctca gttaaaggga cacgccggac gccttttcgg actctcaacg
     1681 gtgtccccca cgcaccggat gagcgcggag gagctgagca acgtcggcaa aatgcacctc
     1741 tacgcggggg gagagcgaaa catcgagaca agatgcctcc tctgcgaaca aacgacacgc
     1801 ggcgataaat accacgcact cggatgtgac gtggtggcaa cgaaccaaca acggcatgcg
     1861 tacctactga gggtactccg gacgtgcatt gcacgacaaa gacctgacct cacggtggtg
     1921 gaaagtgaga tactttaaca gtaacccaga gagttcaagc aagggcaagc ggcaaaccaa
     1981 cgcgagggcg gatatagtcg taagtgcgcg ggaaggagag ggagcgacgt ggctcttgga
     2041 cccgacaatc ggacatgtct tcacggtgga tgtgaagaag atggaagacg taaaggtggc
     2101 gatgctacac gatcacaaaa tgaagaagta caggcgtggc ttcaagatcg acgatgagag
     2161 aacaaacaag ttcctccctg gggcgatgga ctccgtggga cgaatacacg aggagttcga
     2221 ggactacctc gggagtaaag gaaacgacga caacttct
//
LOCUS       DATMTI010000032         7945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_255838, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000032 DATMTI010000000
VERSION     DATMTI010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255838"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /gene="bamA"
                     /locus_tag="VN039_00540"
     CDS             <1..1462
                     /gene="bamA"
                     /locus_tag="VN039_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HWV44509.1"
                     /translation="FTIGEIGFRGNTVYEDPELREGLRIKEGEIFQRAKIRDEITRIT
                     DLYGSKGYSFAEVMPNVSPNNEERTVGLVFNIKEGEMMRIRQINIYGNDKTRDNVIRR
                     EIRVDEQDVIDTASLKRSFQRLNNLNFFETVEILPAQVAPDKVDLNVRVKEKPTGMFS
                     LGGGFSTLDRLVAIADITQGNLGGYGYMGRIRGQLGQRRSLGSITFRNPYLYDSLTSL
                     QIDGYRSITNYLSYFEQRTGGNVTLGRWLSEYVTGSISLFGEEISFSDPQAGLCPDLV
                     PIVCRQLGTRSSTGFRTALFRDTRDYYLDPRSGWRVGGGFDVGTPYLGGTNNFIKYYV
                     DVIKVTPLPFDMRLSLRTRFGEVQALGGTQIPLNERFFVGGINTMRGFSFGRAGPTVP
                     TTHAPYGAAKQLIFSSELIFTVSAEAKLNGVLFFDYGKGFDDNEPLSLNLRPAAGIEG
                     RWISPFGPLRVAYGINLDRRTGERMGVFEFTIGTLF"
     gene            1476..2051
                     /locus_tag="VN039_00545"
     CDS             1476..2051
                     /locus_tag="VN039_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HWV44510.1"
                     /translation="MVRWNGNRRKHSAGLMEWVVMSAAVTITLALSGCAGGAGKIDAK
                     IGVINSQRLLNETSAGKKAKENLTAFSKNRQALMELEEKELRRMEEDFVKQSSVLSPA
                     AKRDREEQFRRRMQEYQQKASELNREVQEKQKDVLEGFRDKIEMIAAKVAKRLGLQVV
                     VDKSKGGPTIYHEEGLDISGPVIEEFNREYP"
     gene            2070..2612
                     /locus_tag="VN039_00550"
     CDS             2070..2612
                     /locus_tag="VN039_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HWV44511.1"
                     /translation="MTRAVRVVVVVAAWSLAQWVEPLHAGEAVRVGVMDQQTVMERTK
                     GGKLALEDVKGYSMTRQKIINADEQELKDLDQSLQDPNAKLTDQARQEKEEQLRSKME
                     AYQRRLQEFNREVQQKQREMVVEYAQKIAAAAKAVAQKEGYTAILDKGNEALIRIVLY
                     YQPALDVTDSVIKEFDRQNP"
     gene            2651..3103
                     /gene="fabZ"
                     /locus_tag="VN039_00555"
     CDS             2651..3103
                     /gene="fabZ"
                     /locus_tag="VN039_00555"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWV44512.1"
                     /translation="MASVEQAEIQALLPHRYPFLLVDRIKEFEPHQRIVGFKNVTVNE
                     PFFQGHFPGRPVMPGVLIIEAMAQAGGVLVFKSGGSVGKTVMYLTGIDEAKFRRPVVP
                     GDQLRLEIEVLKKRPPFWKMQGKAYVDNEVVCEAMVTAMVMEEKTETR"
     gene            3168..3986
                     /gene="lpxA"
                     /locus_tag="VN039_00560"
     CDS             3168..3986
                     /gene="lpxA"
                     /locus_tag="VN039_00560"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWV44513.1"
                     /translation="MKIHPTAIVHPKASLDGDVEIGPFCVIGEHVAIGKGSRLLSHVT
                     IDGWTEIGERNEVHPFASIGGPPQHLGYKGEPTKVIIGHDNIIREYVTMNRGTVQGGG
                     VTSLGSKGILMAYVHVAHDCRLGDHIIMANAASLAGHITIGDHAVIGGLTGILQFVRI
                     GDYVMVGGCCAIGQDIPPFTFAAGGYRAHLYGLNSVGLQRHGFSADRIALLKKAFDLL
                     FRSGHRTAEAIRLAKKEFKGQADVARILTFMEGTKRGISRAVSLDLEREFDQQV"
     gene            4010..4852
                     /gene="lpxI"
                     /locus_tag="VN039_00565"
     CDS             4010..4852
                     /gene="lpxI"
                     /locus_tag="VN039_00565"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HWV44514.1"
                     /translation="MPIPDGRIGVIAGNGRFPIIFADNARKMGLQVFAVAHEGETEPE
                     LEQHVDRIHWVKIGQLNKLINAFKSDGVRNVVMLGGIKKTHVYSHARPDFRVLALATK
                     LVLWKDDDILRALAAELEKDGITICESTFGLEGILVEEGTLASRQPTKKEWVDIRYGW
                     DVAKETGRLDIGQCVVIKDRVVVAVEAVEGTDEAIKRGGELAKDGAVVVKRSKPQQDL
                     RFDLPAVGPRTIEVMRSVKASVLAVEAGRSVMLDRELLISKAEDAGIAIVGLVRETEQ
                     STGT"
     gene            4953..5900
                     /locus_tag="VN039_00570"
     CDS             4953..5900
                     /locus_tag="VN039_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWV44515.1"
                     /translation="MANLRVGVIGVGHLGQHHARLYASLPDSTLIGVVDHDHGRASLI
                     GGRYGARAFDDLPDLLKQVDVVSVAVPTPAHYSVTKTCLEAGKHVLVEKPIAVLPIEA
                     QELVELAKMNGCTLQVGHSERFNPIMQMMRPHIRRPMLFEGQRMGVYSERGTDVDVVL
                     DLMIHDLDLVLSCHPGPVEEVRAAGLSVRSSTNDVANAYIQFESGCVASLIASRVSLK
                     AMRHMRLFQPDESISMDFQSRQGVIGRRSVEPGRKPMVAVEEFQGGDDEPLRLQLESF
                     LQAIHTQSCPVVSGEAGTAALEMAHRVLSAIKAFGERNS"
     gene            5987..7129
                     /gene="lpxB"
                     /locus_tag="VN039_00575"
     CDS             5987..7129
                     /gene="lpxB"
                     /locus_tag="VN039_00575"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWV44516.1"
                     /translation="MARILIISGEASGDLHGANLAKALKASDPHVSLAGIGGRAMEAA
                     GVRLVEEMGRFDVIGMVGPFALVSILRRFITMRRLFRSKQWDTVVFIDHPGLNMRFAY
                     FAKAAGLRVVYYIAPQIWAWAPWRIRWIKQRVDHVLVILPFEKSLYEEAGVRCTFVGH
                     PILDAVPGSYDRRELRSRFGFSDDERVIALLPGSRPHEVQVLLPILLEAAEKLTRREP
                     KTKFILAQASTIHDNLLQPLLVRSPVPIALVKEQATEVMALSDLVLVASGTATLQAAV
                     VGTPMVLFYRTTPLTFWFANVVVRFVVRVKWIGLVNLVAGRTVVPELVQSAATGQRLY
                     EEALRILEDRPTYNEMKRDLAKVRAALGEPGASARAAEVVLTACQA"
     gene            7117..>7945
                     /locus_tag="VN039_00580"
     CDS             7117..>7945
                     /locus_tag="VN039_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWV44517.1"
                     /translation="MSGLDRFKRLMSYVKPYRIRFVGAFVCSGLVALLSGAYAWLARP
                     VLDDIFIQKDERMLLVLPLALLGVATLKAVFSYGVGYLMAYVGNGVVADIRQQLFQQL
                     MRLSVGFHDSNTSGRLVSRIVNDVGMMANAASSVVKDIFQNGLTFLAMVGVIIYQNWR
                     LAAISLVVIPLSALTMVRVGKRLKRLATSGQEQMGDMASTLQETFAGIRVVKAFGREE
                     AEAERFKERNQTFLSTTLKSNQVWSLGHSHMEIIGVIGIAIIIWYGGYLVIHEVMTPG
                     "
BASE COUNT         1797 a         1940 c         2477 g         1731 t
ORIGIN      
        1 gttcaccata ggagaaatag ggtttcgagg gaatacggta tatgaagatc cggagcttcg
       61 agagggattg aggatcaaag agggggaaat ctttcagcgt gccaaaatcc gagatgagat
      121 cacccgaatc acggatttgt acggaagcaa agggtattca tttgccgaag ttatgcccaa
      181 cgtgagtccg aacaatgagg aacggacggt cggcctcgtc ttcaacatca aagaagggga
      241 gatgatgcgg atccggcaga tcaatatcta cgggaatgac aagacacgag acaacgtcat
      301 ccggcgagaa atacgagtgg acgaacagga tgtgatcgac accgcctccc tgaagcgaag
      361 tttccaacga ttgaacaact tgaatttctt cgaaacggtc gaaatcctgc ccgcgcaagt
      421 cgcacccgac aaggtcgacc tgaacgtgcg tgtgaaagaa aaaccgacag ggatgttcag
      481 tctcggcggt gggttcagta cgctggatcg actggtggcg atcgccgata tcactcaagg
      541 caacctggga ggatacggct atatggggcg catccgcggg caactcggcc agcgaagaag
      601 tctcgggtcc atcacctttc gcaatcccta tttgtacgac tccttaacgt cgctgcaaat
      661 agacggttac cgcagtatca cgaattacct atcatatttt gagcaacgaa ccggcggaaa
      721 cgtcacgctc ggtcgttggt tatcggaata tgtgacgggg agcatcagtc tctttggaga
      781 agaaatctcg ttttcagacc ctcaagcggg tctttgcccg gatctagtgc cgatcgtgtg
      841 tcggcagttg ggaacacgat cgtcgacagg atttcggacg gccctgttcc gtgataccag
      901 agactactac ctcgatcctc gatccggttg gcgtgtcggc ggaggctttg atgtcggaac
      961 accctacctg ggcgggacca acaacttcat caagtattat gttgatgtca tcaaggtcac
     1021 gccgcttccg ttcgatatga ggctgtcgct gcgtacgcgt ttcggcgagg tccaggcact
     1081 cggcggaacg cagattccac tgaatgaacg gttcttcgtg ggcggcatca ataccatgcg
     1141 tggattttcc ttcggtcgag ccggtcccac agttcccacg acccatgctc catacggcgc
     1201 ggccaagcaa ttgatcttca gcagtgagct gatctttacg gtctctgccg aggcaaaatt
     1261 gaacggcgtg ctcttcttcg actacggaaa ggggtttgat gacaatgaac cgttgtcgct
     1321 caatttgagg cctgctgccg ggatagaggg acgctggata tccccatttg gtccgttgcg
     1381 tgtcgcctat gggatcaatc ttgatcggcg gaccggtgag cgaatgggcg tgttcgagtt
     1441 tacgatcggg accttgtttt agtcggagcc ttggcgtggt acgttggaac gggaatcggc
     1501 ggaagcactc ggcgggactg atggaatggg tggtgatgtc ggctgcggtc accatcacgc
     1561 tggctctcag cggatgtgcc ggaggcgcgg gcaaaatcga cgcaaagatc ggggtgatca
     1621 attcacagcg cttgctcaac gagacgagtg cgggcaaaaa ggccaaagag aatttgaccg
     1681 ccttttcgaa aaatcgacag gcgctgatgg aattggagga aaaggagttg cggaggatgg
     1741 aggaggactt tgtcaagcag tcttccgtcc tcagccctgc cgcgaaacga gatcgggaag
     1801 aacagtttcg ccggcgcatg caggagtacc agcaaaaggc ctcggaattg aatcgagaag
     1861 tccaagaaaa acaaaaagac gttctggaag ggtttcgtga caagatcgaa atgatcgccg
     1921 caaaggtcgc caagcgtctc gggttgcagg ttgtcgttga taagagcaag ggtggtccga
     1981 ccatctatca tgaagaaggt ctggacattt cagggccggt catcgaggaa ttcaaccgcg
     2041 aatatcccta gtgctgcggg aggtagatta tgacgagagc agtgagagtg gtagtggtgg
     2101 tcgccgcatg gtcattggcg cagtgggttg agccgctcca tgccggtgaa gcggtccggg
     2161 tgggggtcat ggatcaacag acggtgatgg aacgaacgaa gggcggaaaa ctggccttgg
     2221 aagatgtgaa gggatattcc atgaccagac agaagatcat taacgccgat gagcaagagc
     2281 tcaaagattt ggatcagtcc ttacaagatc cgaacgccaa actcaccgac caagcgagac
     2341 aagagaaaga ggagcagctg cggagtaaga tggaagcgta tcagcgccgc ctgcaagagt
     2401 ttaatcgtga agttcaacaa aagcagcgcg agatggtcgt ggaatatgcg caaaagatcg
     2461 cggccgcagc gaaggccgtg gctcagaaag agggatatac ggccatcctc gataaaggca
     2521 atgaggccct catccgcatc gttctctatt atcagccggc gctggacgtg acggactcgg
     2581 tcatcaagga atttgaccgg cagaatcctt aaaccgtaga actatccggg acagagagga
     2641 ggcagagcca atggcatcgg tcgagcaggc tgagattcag gcgttacttc cccaccggta
     2701 tcccttcttg ctggtggatc gaatcaagga gtttgaaccg catcagagaa tcgtcgggtt
     2761 caaaaacgtg acggtgaatg aaccgttctt tcaaggacat ttccccggac gtcctgtgat
     2821 gccgggagta ctgattatcg aggctatggc gcaggcaggc ggcgtgctgg tcttcaagtc
     2881 gggggggtcg gtcggaaaaa ccgtcatgta tctgacgggt atcgacgagg caaaatttcg
     2941 gcgaccggtc gtgccgggcg atcagctccg gcttgaaatc gaagtactca agaagcgtcc
     3001 gccgttctgg aaaatgcaag ggaaggcata cgtagacaat gaagtggtct gtgaagccat
     3061 ggtgacggcg atggtgatgg aagagaagac ggaaacacgg taagcaaggc agtgacccgg
     3121 ggttgaccaa tgaccggtga tgttggacgc gagtaaaggg ggcatgcgtg aagatacatc
     3181 ctacagcgat cgttcatccc aaggcgagtc tcgacggcga cgttgagatc ggaccatttt
     3241 gtgtaatcgg ggagcatgtc gcgatcggaa agggaagccg gttgctctcc catgtgacta
     3301 tcgatgggtg gacggagatt ggggagcgga acgaagtgca tccgtttgct tcgatcggag
     3361 ggcctccaca acaccttgga tataaggggg agccgaccaa ggtcatcatc gggcatgaca
     3421 acattatccg ggagtatgtc acgatgaatc ggggaaccgt ccaagggggt ggagtgacct
     3481 ctcttgggtc gaagggcatt ctgatggctt acgtgcatgt ggcacacgat tgccggctcg
     3541 gcgaccatat tatcatggcc aatgcggcaa gccttgccgg acacatcacc atcggtgatc
     3601 atgccgtcat cggaggattg accggtattc ttcagtttgt gcgcatcgga gactacgtga
     3661 tggtcggcgg ttgttgcgcc attggtcagg atattcctcc atttacgttc gccgccggcg
     3721 ggtaccgagc ccatctgtac ggtctgaatt cggtcggcct ccagcgccat ggtttttcag
     3781 ccgatcgcat cgccttgctg aagaaggcct ttgacctgct ctttcggtcc ggccaccgaa
     3841 cggccgaggc gattcgactg gccaaaaagg aatttaaggg acaggctgat gtggccagga
     3901 ttctcacgtt tatggagggt accaaacgtg gaatctcgcg ggctgtcagc ctcgacttgg
     3961 agcgcgagtt cgatcaacaa gtgtgatgtc tgccgctatg cacctgacca tgcctatacc
     4021 agacggtcga atcggggtga tcgccgggaa tggacggttt ccgattatct ttgcggataa
     4081 cgcccgcaag atggggctcc aagtattcgc ggtggctcac gagggcgaaa ccgaaccgga
     4141 gctcgaacaa catgtcgacc ggatccattg ggtcaagatc ggccaactca ataagctgat
     4201 caacgcgttc aagtccgacg gcgtccggaa cgtggtcatg ttgggaggca tcaagaaaac
     4261 gcatgtctat agccatgcac gtcctgattt tcgcgtgctg gcgttggcca cgaaattggt
     4321 cctctggaaa gatgatgata tcctacgcgc actcgcggca gaactcgaaa aggatggcat
     4381 caccatctgt gagtcgacgt tcggcctgga agggatcttg gttgaagaag gcacgctcgc
     4441 gtcccgccaa ccgaccaaga aagagtgggt tgacatccgt tatggctggg acgtggccaa
     4501 agagacgggg cgcctcgaca tcggccaatg tgtcgtgatt aaagaccgtg tcgtcgtggc
     4561 ggtcgaagcg gtcgagggaa ccgatgaagc gatcaagcgt ggaggcgaac tggccaagga
     4621 tggtgcggtg gtggtgaagc ggagcaagcc ccagcaggat ctccggtttg acctgccggc
     4681 cgtcggcccc agaacgatcg aggtcatgcg ttccgtcaag gcctcggtgc ttgcagtcga
     4741 ggccggtcgg tccgtgatgc tggatcgaga actgctgatc agcaaggcgg aagacgccgg
     4801 aatcgccatc gtcggcctcg ttcgcgaaac ggagcagagc acggggacct gagtccgcgg
     4861 agaagagctt cggaatctga agatctattt gatttcagaa acttaacaaa ttaatcgatc
     4921 aagagagtcc ccaatttcag tccaagcggt gtatggccaa cctgcgtgtc ggtgtaatcg
     4981 gcgtcggaca tctcgggcaa caccacgcgc gactgtatgc ctcattgccc gactcgacct
     5041 tgatcggcgt ggtcgatcac gaccatggac gagcttctct catcgggggg cgatacggcg
     5101 cgcgggcgtt tgacgacctg ccggatcttt tgaaacaggt cgatgtcgtc agtgttgccg
     5161 tcccgacacc cgcacactat tccgttacca aaacctgtct cgaagccgga aaacatgttc
     5221 tggtagaaaa gccgatcgcc gtgctgccga tcgaagcaca ggagctggtg gagttagcca
     5281 agatgaatgg atgcacgttg caagtcgggc atagcgaacg gttcaacccg atcatgcaga
     5341 tgatgcggcc gcacattcgg cggccgatgt tgttcgaggg gcagcggatg ggggtttaca
     5401 gcgagcgcgg cacagacgtc gatgtcgtct tggatttgat gattcacgac ctggatttgg
     5461 tgttgtcctg ccatccaggc ccggtggaag aagtacgagc cgccggcctg tcggtacgtt
     5521 cttctacgaa cgatgtcgcc aatgcgtata tccaattcga aagcgggtgt gttgccagct
     5581 tgatcgcaag ccgagtctcc ttgaaggcaa tgcggcatat gcgtctgttt caaccggatg
     5641 agtctatctc tatggatttc caatcccgcc agggggtaat cggacgccga tcagttgagc
     5701 caggacggaa accgatggtg gcggttgaag agtttcaggg cggcgatgat gagcccctga
     5761 gattacagct ggagtcgttc ctgcaggcca ttcatacgca atcatgcccc gtcgtgtcgg
     5821 gcgaagctgg gacggccgcc ctggagatgg cgcatcgcgt gctgtccgcc attaaggcgt
     5881 tcggagagcg taactcctga gaacatcttc tgtcggtcga ccatatcggg ttcctcgcgg
     5941 tcatgtgcac caggccgccg tcatgtttat ggccgtatag atcatcatgg cccgtatcct
     6001 gattatttcc ggtgaagcct ccggcgatct tcatggagcc aatcttgcca aagcgctgaa
     6061 agcgagcgat cctcacgtct cgttggctgg aattggtggt cgcgcgatgg aggcggcagg
     6121 tgtgcgattg gttgaggaaa tggggcgttt tgacgtcatc ggtatggtcg gccctttcgc
     6181 gttggtgtcg atcttacgtc ggtttatcac gatgcggcgg ttattccgat caaaacaatg
     6241 ggacacggtg gtgtttatcg accatccggg tctcaacatg cgctttgcgt attttgccaa
     6301 ggcggccggc ctgcgggtgg tgtactacat cgcgccgcag atttgggcgt gggcgccttg
     6361 gcggattcgt tggatcaaac agcgggtcga tcatgtcctg gtcatactcc cttttgagaa
     6421 gtctctctat gaagaagctg gcgtacggtg tacgtttgtc ggccatccca tattggatgc
     6481 ggtcccaggc agctacgatc ggagggagct tcgcagcaga ttcggtttct cggatgacga
     6541 gcgagtgatt gccttgttgc cgggaagccg gccccatgag gtgcaggttc tactgccgat
     6601 cctcctcgaa gctgcagaga aattgacacg ccgggaaccc aaaacgaagt ttatcctggc
     6661 gcaagcctcc accatacacg ataatctgtt gcagcctctc ctggtgcgaa gcccggttcc
     6721 tatcgcgctg gtgaaggagc aggccaccga agtgatggca ttgtcggatc ttgtgctggt
     6781 ggcttcgggt acggctacgc tgcaagcggc tgtagtcgga actccgatgg tgttgttcta
     6841 tcgaaccacg ccgctgacct tttggtttgc gaacgtcgtg gttcgattcg tggttcgagt
     6901 caaatggatc ggccttgtca atctggtggc cggccggacg gtcgtcccgg agttggtcca
     6961 atccgctgcg acggggcagc gattatatga agaagctctt cgaatcttgg aagatcggcc
     7021 aacatataat gagatgaagc gggatttggc aaaggttcgg gccgccttgg gtgaaccggg
     7081 cgcgtcggcc agagctgcag aggtggtgtt gacggcatgt caggcttaga tcgattcaag
     7141 cggctgatga gttatgtgaa gccgtaccgt atacggttcg taggagcctt tgtctgttca
     7201 ggtctggtag cactgctcag cggggcctat gcctggctcg cgcgcccggt tttggacgat
     7261 atcttcattc aaaaggacga acggatgttg ctggtgctgc cgctggcact cttaggggtc
     7321 gcgacactca aggcggtgtt cagctacggc gtgggatatt tgatggccta tgtcggaaac
     7381 ggggtcgtgg cggacatccg acagcaattg ttccaacagc ttatgcggct gtccgtcgga
     7441 tttcatgact ccaatacatc ggggcggctg gtctcgcgga tcgtgaacga tgtgggaatg
     7501 atggcaaatg ccgcatcgag cgtcgtcaag gacattttcc agaacggtct cacctttttg
     7561 gcgatggtcg gagtcatcat ctatcagaat tggagactgg cggcgatctc tttggtcgtg
     7621 atcccgctct cggcattgac aatggtgcgc gtggggaaac gattgaagcg attggcgacc
     7681 agcggacagg aacaaatggg ggatatggcc tcaacgctcc aagaaacgtt cgccggtatc
     7741 cgagtcgtga aagcgttcgg gcgtgaggag gcggaggctg aacgattcaa ggaacgaaac
     7801 cagacctttc tctcgaccac gttgaagtcc aatcaggtct ggtcgctcgg ccactcacac
     7861 atggaaatca tcggagtgat tggtatcgcg atcattattt ggtatggagg ctatctggtc
     7921 attcatgagg tgatgactcc tgggg
//
LOCUS       DATMTI010000033         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_213567, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000033 DATMTI010000000
VERSION     DATMTI010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213567"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..2519
                     /gene="gyrB"
                     /locus_tag="VN039_00585"
     CDS             51..2519
                     /gene="gyrB"
                     /locus_tag="VN039_00585"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HWV44518.1"
                     /translation="MTTDDSSQPKSDSYSADQIKVLEGLDAVRKRPAMYIGSTGVDGL
                     HHLVYEVVDNSVDEHMAGFGEAIEVTIHIDGSVTVIDNGRGIPTGMHPTQKKSAAEVA
                     LTVLHAGGKFEQGAYTVSGGLHGVGISVVNALSEWLELEIWQDAQVFEQRYERGKPSA
                     PLNATGKTKRRGTKVRFKPDGQIFETLEFSFDVLAQRLRELAFLNKGLAITLRDERKE
                     PAKEQVFLYKGGIVSFVEHLNEAKTPLHKPIYVKVEKPEMILEVALQYNDGYAENLFS
                     FANNINTKEGGTHLVGFKAALTRTINNYANANDLFKKETESLTGDDVREGLTAVVSVK
                     VRNPQFEGQTKAKLGNSEVKGVVEAAVNEALGNYFEENPPVARKIIGKAVDAARAREA
                     ARKAKDLIRRKSALDGGSLPGKLADCSEKDPALSELYIVEGDSAGGSAKQGRDRKFQA
                     ILPLKGKILNVEKARFDKMLSSDEIRTLIMALGTGIGRKREESDKSEKDTFDIAKARY
                     HKIILMTDADVDGSHIRTLLLTFFFRQMPELLERGYIYIAQPPLFKVKKGKTERYLKD
                     EGSLNEHLADLAVEDVELYMEGAQGYVTGRRLLPILKKMISFETLLGRLNKKPHEAAM
                     LRAFVDEPGLDRELLKDREALNRVVVGVKKALLLAFPKLEPTFEIFTDEEHQSSKVTC
                     RLHANGMVHSIEINHDLVGSADFRELQKLAPSVIGLGRAPYRLKTKGQERQLVTTAET
                     VRTILELGKQGLGIQRYKGLGEMNPSQLWETTMDPEKRTLLKVQLEDVTGVDEIFTIL
                     MGDEVEPRRNFIQEHALEVRNLDV"
     gene            2678..>3229
                     /locus_tag="VN039_00590"
     CDS             2678..>3229
                     /locus_tag="VN039_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWV44519.1"
                     /translation="MPHDERLEHIDIEDEMRSSYLSYAMSVIVGRALPDVRDGLKPVH
                     RRILYGINEMGLASNRAYRKSAKIVGEVMGNYHPHGNMPIYDALVRMAQDFNMRYPLI
                     DGQGNYGSMDGDPPAAERYTEARMTKLAEEMLADIDKETVDFGPNYDESRQEPLVLPT
                     RVPNLLINGAGGIAVGYATNIPTH"
BASE COUNT          817 a          850 c          944 g          618 t
ORIGIN      
        1 atgctgaaaa agtccatcaa catcgttctc gtatcgacca taggaaacgg atgaccacag
       61 acgactcttc ccaacccaaa tcagacagct acagcgcgga tcagatcaag gtcctcgaag
      121 gtctcgacgc cgtacgaaag cgcccggcaa tgtacatcgg gagcaccggt gtcgacggac
      181 tccaccatct ggtctatgaa gttgtcgaca acagtgtcga cgagcatatg gcggggttcg
      241 gtgaggcgat cgaggtgacg atccacatcg acggaagcgt gacagtcatc gataacggcc
      301 gcggcattcc caccggcatg catcccacgc aaaagaaatc cgcagccgag gtggcgctga
      361 ccgtgcttca tgcaggcggc aaattcgagc agggagccta caccgtctcc ggcggcctac
      421 acggcgtcgg catctccgtc gtgaacgcct tgtcggagtg gctcgagttg gagatctggc
      481 aggatgccca ggtattcgaa caacgatatg aacgcggcaa gccgagtgcg ccgctcaatg
      541 ccacgggtaa aacaaagcgc cggggcacca aggttcggtt caagccggac ggccaaatct
      601 tcgagacgct cgaattcagc ttcgacgtgt tggcccagcg gcttcgcgag ctggcctttt
      661 tgaacaaagg cctggccatt accctgagag atgagcggaa agaaccggcg aaagagcaag
      721 ttttcctata caagggcggc atcgtctctt tcgtggaaca tttgaacgag gcgaaaacgc
      781 cgctgcacaa gcctatctat gtgaaagttg agaagccgga aatgattctg gaagtggcac
      841 tccagtacaa cgacgggtac gcggagaacc tattttcatt cgcgaacaac attaatacca
      901 aggaaggcgg tacccatttg gtggggttta aagccgccct cacgaggacg atcaataatt
      961 acgccaatgc aaacgatctc ttcaagaagg aaaccgaatc gctgaccgga gatgatgtgc
     1021 gggagggtct cacggcggtc gtcagcgtca aggtccgtaa tccgcagttc gagggccaga
     1081 cgaaagccaa gctcgggaac agcgaagtga aaggcgttgt cgaggctgct gtcaacgaag
     1141 ccttgggcaa ttatttcgag gaaaatcctc ccgtcgctcg aaagatcatc ggcaaggctg
     1201 tcgacgcagc ccgtgcgcgc gaagcggccc ggaaagccaa ggatttgatc agacgcaaga
     1261 gcgcgctcga cggcggctcg cttcccggca agttggccga ttgttcggaa aaagatccgg
     1321 ccttgagcga actctacatc gttgaaggcg attcggccgg tggatctgcg aagcaaggac
     1381 gcgaccgcaa gttccaagcg atcctgcccc taaaagggaa gatcttgaac gtcgagaagg
     1441 cacgtttcga caagatgcta tcgagcgacg agatcaggac gctcatcatg gcgctgggga
     1501 ccggtatcgg ccgcaagcgg gaagagagcg acaagtcgga gaaagacacg ttcgatatcg
     1561 caaaggctcg ctatcacaag atcattctca tgaccgatgc cgacgtcgac ggcagccaca
     1621 tccgtacctt gctcttgacg ttcttcttcc ggcagatgcc ggaattgctc gaacggggtt
     1681 atatctacat cgctcaacct ccgctgttca aagtgaaaaa aggcaagacg gagcggtatc
     1741 tcaaggatga ggggtcgttg aacgagcatt tggccgactt ggcggtcgag gacgtcgaac
     1801 tgtacatgga aggggctcag ggatatgtga ccggacgccg gttgctgccg attctcaaga
     1861 agatgatctc gttcgaaacg ttgctcggtc gcctcaataa aaagccgcat gaagcagcca
     1921 tgctgcgcgc ctttgtcgat gaaccggggc tcgatcgtga gctcctcaag gaccgagagg
     1981 cactcaatcg cgtggtagtc ggggtgaaga aggctctcct cctggcgttc ccgaagttag
     2041 aaccgacgtt cgagatcttt acggatgaag agcatcagtc cagcaaagtc acatgccgac
     2101 ttcatgccaa cggcatggtg catagcatcg aaattaacca tgacctggtg ggatcggccg
     2161 attttcgtga gcttcaaaag cttgcgccgt ccgtcatcgg actgggtcgg gctccgtaca
     2221 ggctgaaaac caagggtcag gaacggcagc tggtgaccac agctgaaacg gtcaggacga
     2281 tcctcgaact cggaaagcag ggtcttggta ttcagcggta caaaggactt ggtgaaatga
     2341 acccgtccca attgtgggaa acgacgatgg accctgagaa acgcacgctc ttgaaggttc
     2401 agctggagga cgtgaccggc gtcgatgaaa tcttcacgat tctgatgggc gacgaagtcg
     2461 agccgcgaag aaatttcatc caggaacatg cgctcgaagt ccggaacttg gacgtgtagg
     2521 gggtcggcga gaccgtttag aaacaagcac tctgcattaa tcttgtcgga tgctcaaatg
     2581 gtcatccggc aaggccgcag gcgtaccttc tgggtacgtt gaggatcttt cgatccgaga
     2641 acgaagtcgg tgattatttt cagcatccga ggacagaatg cctcacgatg aacgtttaga
     2701 acatatcgac atcgaagatg aaatgcgctc gtcgtacttg agttacgcga tgagcgtcat
     2761 cgtaggaaga gcactgcccg acgtccgtga tgggctcaaa cctgttcatc gccgcatcct
     2821 gtacggcata aatgaaatgg gtctcgcctc caatcgggcc taccgcaagt cggccaagat
     2881 cgtgggcgag gtcatgggga attatcatcc gcatgggaac atgcccatct acgacgcgct
     2941 cgttcgaatg gcgcaagact tcaacatgcg ctatccgctc atcgacggtc agggcaatta
     3001 tgggtcgatg gatggtgatc cgccggccgc agaacggtat accgaagcgc gcatgaccaa
     3061 actggccgaa gagatgttgg ccgacatcga caaagaaacg gtcgatttcg gtccgaacta
     3121 cgacgaatcg agacaggaac ccctggttct accgaccagg gtgccgaatc tcctcattaa
     3181 tggagcaggc ggcatcgctg tcggctatgc cacgaacatc ccgacgcac
//
LOCUS       DATMTI010000034         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_128844, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000034 DATMTI010000000
VERSION     DATMTI010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128844"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VN039_00595"
     CDS             complement(<1..600)
                     /locus_tag="VN039_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HWV44520.1"
                     /translation="MKRKRQDERVIVGVIGGSGLYDIEGLTSTRSIRVRTPFGAPSDA
                     ITVGLLEGIRVAFLSRHGRGHVLNPSGINYRANIFALKSLGVSHVISVSAVGSMKESI
                     HPGDVVVPDQFIDLTKRRVSTFFDEGVVAHVAFGEPICAELGQTLLAAGEKVGAKLHR
                     SGTYLCMEGPQFSTKAESRLYRQWGVDVSGMTNMPEAKLA"
     gene            complement(614..1621)
                     /gene="miaA"
                     /locus_tag="VN039_00600"
     CDS             complement(614..1621)
                     /gene="miaA"
                     /locus_tag="VN039_00600"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWV44521.1"
                     /translation="MTKLSDIICGHRPLVVLLGPTAVGKSRIAIQVAKHFDTDVLAAD
                     SRQVYRGMDIGTDKPTTEERQGVPHRLIDLVAPSEAFNAGWYRRVAMAEIERLYNAGR
                     LPFVVGGTGLYIRTLVKGLCSAPQADPDVRADLRKLRDDGGRDGLYAELMRVDPETAT
                     RLHPNDESKVMRALEVYRLSGRPLSTMQAEHGFHETPFSALLIGLERRKENLYRRIEE
                     RIDWQLTHGMVEETRLLLGQGYGRELGSMKGLGYRQVGAYLANECDYAEMVRRFKRDT
                     RRFAKRQMTWFRSETGVAWLPIEDNEPYERTAERVIARIEQFMKALVQPKQPAGLQQA
                     F"
     gene            complement(1732..>3559)
                     /gene="mutL"
                     /locus_tag="VN039_00605"
     CDS             complement(1732..>3559)
                     /gene="mutL"
                     /locus_tag="VN039_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWV44522.1"
                     /translation="WRDAPPHHRLFAHEEHRAVLRTDGSGKICILPEEVIGRIAAGEV
                     VERPAAVVKELLENSLDAGSRSITIDVRDGGLALIRVSDDGEGMSREDAPRAFQRHAT
                     SKLQSDLDLWSIRTMGFRGEALPSIAAVAHVRLMTATRSADVGTELEVVGGAIGRIAE
                     APPITGTRIEVAELFHNQPARKKFLKSTLTEFLHISRVVQQASLAWPSIHFRLTHNAE
                     EIINYPSAPSDDDRIAQVYRRTFLDQCLRIEASLPGVSVIGHIVDAVHAKPTRIPQEL
                     FVNRRPVRNAAISHAVGEGYGSFLAKGSQPRFVLFLEIDPDRLDVNVHPTKREVRFSD
                     NELIHQLVRRAVRQALSGGKTDELGKAPFVESSVRRTPGSIVLALPTSESVEAESTTL
                     QPSPETQLPLASESAEPYGQVPSTEVTALGQINRTFLIAQVGGDLTVIDQHTAHERVL
                     FERLCRAWTTRGIQAQPLLIPDPVELSPSQTALFQRYQSDLEQLGVDVEPFGSSTVLI
                     RAMPVGLGKIDPTTFLEDLMDDLVQWDSVSSVEARVRSVLASLACHGAVRAGRSMKPE
                     EIKVLIEDWRAEGEITTCPHGRRTSFRLSITDLEKMFGRAGW"
BASE COUNT          751 a         1106 c          935 g          767 t
ORIGIN      
        1 ggccagtttc gcttccggca tatttgtcat gccgctcaca tcgacgcccc attgccgata
       61 gagccgtgat tccgccttcg tcgaaaactg cggcccttcc atgcagagat aggtcccact
      121 gcggtgcaac ttggcgccga ccttttctcc agccgccagg agagtctgtc ccagttcggc
      181 gcaaatcggc tcgccgaagg cgacatgcgc caccactccc tcgtcaaaaa atgtcgagac
      241 gcggcgcttc gtgaggtcga tgaactggtc cggcacaacg acgtcgcccg ggtgaatgga
      301 ttctttcatg ctgcctaccg cgctgaccga aatcacatga gacactccca gagacttgag
      361 cgcgaaaata ttcgcgcggt agttgatgcc gctcggattc aacacatgtc cccgcccatg
      421 ccgtgaaaga aatgcgactc gaattccttc cagcaaaccc acggtaatgg catcggaagg
      481 agcaccgaaa ggcgtacgga ctcggatgga ccgagtcgag gtgagtcctt cgatgtcata
      541 cagcccgctt cctccgatca ctccgacgat cacccgctca tcctgccttt ttctcttcat
      601 agagatcctt ttcttaaaag gcctgctgca atccagcagg ttgtttaggt tgtacaaggg
      661 ctttcataaa ttgctcgatg cgtgcgatca ctcgttccgc cgtccgctca tacggctcgt
      721 tgtcctcgat cggcaaccat gctactcccg tttcgcttcg aaaccaggtc atctgccgtt
      781 tcgcaaaacg tctcgtgtcg cgcttgaatc gacgcaccat ttcagcgtaa tcgcactcat
      841 tcgccaaata ggctccgacc tggcgatagc cgagaccttt catcgaaccg agctcgcgtc
      901 cgtatccctg accgagcaat aatcgagtct cttctaccat tccatgagtc agctgccaat
      961 cgattcgttc ctcaatcctt cgatacagat tctctttcct tcgctcaagg ccgatcagaa
     1021 gagcggaaaa cggagtctcg tgaaatccgt gttcggcttg catggtcgac agtgggcgcc
     1081 ctgatagccg atagacttcc aatgcgcgca tgactttgga ttcatcatta gggtgcaacc
     1141 gtgttgccgt ttcagggtcg acacgcatca actctgcata gagaccgtct cgccccccgt
     1201 catctctcaa tttcctaagg tctgccctca catccgggtc ggcttgaggc gccgaacata
     1261 accccttcac taaggttcgg atatacaacc ccgttccacc caccacgaag ggcaaccgtc
     1321 cggcattgta taaccgttcg atttctgcca tcgcgacccg ccgataccaa ccggcgttaa
     1381 acgcttcgct gggggcgacc aaatcgatca gtcggtgcgg caccccctga cgctcctcgg
     1441 tggtcggctt atccgttcca atgtccatcc cgcgatacac ctgccgagaa tccgccgcca
     1501 acacgtcggt atcaaagtgt ttggccactt gtatggcaat ccgactctta ccgacggccg
     1561 tcggaccgag aagcacgact aggggacggt gtccgcatat gatgtccgat aactttgtca
     1621 tatttcatca gcgatgatgt gccttttgaa agagtgcgac tgaggagagc gtgaagaaag
     1681 ccattcgaat ctgatcgaat atggtctcag cctaaacctt gaccttgctc gctaccagcc
     1741 ggctcgtccg aacattttct ccaagtctgt gatgctgagt cggaacgatg ttctccgtcc
     1801 atgtggacag gtcgtgatct ccccttcggc tcgccagtcc tcaatcagaa ctttgatttc
     1861 ttccggtttc atcgagcgac cggcccgaac cgcgccgtgg cacgccaacg atgccaggac
     1921 cgatctcact cgtgcctcca cactagagac gctgtcccac tgcacaagat cgtccatgag
     1981 atcctccaga aacgtcgtag gatcgatctt accgagaccg accggcatcg ccctgatcaa
     2041 gacggtggag gaaccaaatg gttcaacgtc cacccccaat tgctccaggt cactttggta
     2101 acgttgaaac aatgccgtct gagatggcga cagttccacc ggatcgggga tcaacagagg
     2161 ctgggcctgg ataccgcggg tcgtccaagc gcgacacagc cgctcaaaca ggacccgctc
     2221 gtgagccgtg tgttgatcga tcaccgtcaa atcgcccccg acttgagcga taaggaatgt
     2281 cctattgatc tgccccagcg cggtcacctc agttgaaggc acttgaccgt agggctcagc
     2341 cgattcgctc gccaacggca attgagtttc ggggcttggc tgcagggtcg tcgattccgc
     2401 ttcgacggat tcggatgtgg ggagagcgag cacgatggaa cccggggtcc gtcgcacgga
     2461 ggactctacg aatggagcct tccccaactc atccgtcttt ccgccactca aggcttgacg
     2521 gaccgctcgc cgtacgagtt ggtggatcaa ctcattgtcc gaaaatctca cttcccgctt
     2581 ggtcggatgg acattgacat cgaggcggtc cggatcaatt tccagaaaca gcacgaatcg
     2641 cggttggctc cccttggcga ggaatgaacc atacccttcc ccgacagcat gagagattgc
     2701 ggcgttgcgc acaggacgac ggttcacgaa taactcttgc ggtatgcgag tgggcttggc
     2761 atgaaccgca tccacgatgt gcccgatgac agagactcca ggaagcgagg cctcgatccg
     2821 gaggcactgg tcgaggaagg tgcgccgata gacctgggcg atccgatcat catccgatgg
     2881 cgcagacggg taattgatga tctcttcagc gttgtgcgtc aaacggaaat ggatggatgg
     2941 ccaggccagc gacgcctgct gtacgacccg gctgatatgc aagaactccg tgagggtcga
     3001 cttcaggaac ttcttccggg cgggctgatt gtggaacagc tctgcaactt caatgcgtgt
     3061 tcctgtgatc ggaggagctt cggcgattct cccgatcgct ccccccacaa cttccaactc
     3121 ggtccccacg tcggcagaac gagtcgccgt catcagtcga acatgggcga cagcggcgat
     3181 gctcggcagc gcttcgcccc gaaaacccat cgttcggatg gaccagagat ccagatcgga
     3241 ctgcagcttg ctcgtggcat gccgctgaaa cgcgcgcggg gcatcctccc ggctcatgcc
     3301 ctctccgtcg tcgctcaccc gaatcaaggc gagccccccg tccctcacgt cgatcgtgat
     3361 ggaccgactc cccgcatcga gactgttctc gaggagctct ttgacaaccg ccgccgggcg
     3421 ttcaacgact tctcccgctg caatgcgacc gatgacctct tccggaagaa tacagatctt
     3481 accactgccg tcggttctca gcacggcacg atgctcctca tgagcgaaga gacggtgatg
     3541 gggcggggcg tcgcgccac
//
LOCUS       DATMTI010000035         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_510649, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000035 DATMTI010000000
VERSION     DATMTI010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510649"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VN039_00610"
     CDS             <1..217
                     /locus_tag="VN039_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44523.1"
                     /translation="AIPAIKLDPESAGLLPRSIRVPLHIKVKSGQTRRVQVAPRGLVG
                     EENLGARYQFFCPKHKDIRVSRFIYVD"
     gene            753..1523
                     /locus_tag="VN039_00615"
     CDS             753..1523
                     /locus_tag="VN039_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HWV44524.1"
                     /translation="MRTSKVNAWYGWVPDRPDQRDKLYAAIAAPPKTLPPKVDLRDDC
                     SPIEDQGQLGSCTANALVGNLEFLEKKAGHNPANLSRLFIYYNERAMEGTISEDAGAM
                     IRDGVKSLVKWGVCTEKRWPYKINKFAKKPSPSCYKQAGDYQVTSYHRIIGLQQMKQC
                     LAEGYPFVFGFSVYEAFESPHVAKTGQLNVPKPGEKQLGGHAVMAVGYDESVRRMLVR
                     NSWGTDWGIQGYFTMPYDYISNDNLADDFWTLRAFEGE"
BASE COUNT          533 a          549 c          586 g          440 t
ORIGIN      
        1 ggcaatcccc gcgataaagt tggatcctga atccgcaggg ctactcccca gaagcatccg
       61 ggtaccgctt cacatcaagg taaagtctgg acagacgagg agagtgcagg ttgcgccacg
      121 cgggttagtt ggtgaggaaa acctgggggc tcggtatcag ttcttttgcc ccaagcacaa
      181 ggatatccga gtcagcaggt tcatctatgt ggactagacg tcaggtcagc agcacatacc
      241 gatcatcatg aggcgcacgc cgtttaatca cccatcgtct ttgttctaat gcacggacgg
      301 gatagccgac ggttcggttc gtcagaccaa acgcccttgt acactgcgta atgtccgccc
      361 gactggcttg acagcttaaa ctttggtgag gaaccggaac cgatatcggt gctcccgatt
      421 tgtgtgcgtg tggtgtggga gcggaacaca ggcgtccaga gcgggtctca atttatggtg
      481 gagtggtcgt acgctacaca tcgctcgccc gaataggaat ggaggtcgtc tgcaaatgcg
      541 aatacgcgaa gactgcacgt atagatccat tcagcacgac tacggataga tacaatggtc
      601 cttacctaga ggatgggatt gcagcgtgaa cgcgaatctt ttagcattcg gtgcgactcc
      661 cgagggcaca tgtatgtagg taatcacatg cctggtcaag aagccgcata ggcggtggta
      721 tgacaagcgg cgcacaattg taggaggatg atatgcggac atccaaagta aatgcctggt
      781 acggatgggt tcccgaccgg cccgatcaac gggacaagtt gtacgccgcc attgccgcac
      841 cgccgaagac cctaccgccg aaggtggatc ttcgcgatga ctgctcgcct attgaagatc
      901 agggacaact cggcagttgc acggccaacg cgctggtggg caatctggag ttccttgaaa
      961 agaaagccgg gcacaaccct gcgaacctga gccgactgtt catttactac aatgagcgcg
     1021 cgatggaagg caccatcagc gaagacgccg gcgccatgat ccgcgacggc gtcaaatccc
     1081 tcgtcaaatg gggggtctgt accgagaaac gctggccgta caaaattaac aaatttgcga
     1141 agaaaccgtc gccgtcctgt tacaaacagg ctggcgatta tcaggtcacc tcctatcacc
     1201 ggattatcgg cttgcaacag atgaagcaat gcctcgcgga aggctacccg ttcgtgtttg
     1261 ggttttctgt ctatgaagcc ttcgagtctc ctcacgtggc caagaccggc cagttgaacg
     1321 tgcccaaacc aggggaaaag cagcttggtg gccatgccgt gatggccgta ggatatgatg
     1381 agtcggtccg gcgcatgctc gtccgcaact cctggggcac ggactggggc atccagggat
     1441 atttcacgat gccttatgac tatatctcga atgacaacct cgcggacgac ttctggacgc
     1501 tccgcgcgtt cgagggcgag taaatattgc tggcataacg tccaccgatt ccgcgaaagg
     1561 aactttcatt ccagccacta tgctgagcat gaatgaaggc ggatggacaa ggaaatatag
     1621 atggaaacac agtaggctca acacacacgt cgtagaatga gaggttgata gggcaatgtt
     1681 ggtcgtcgcg agggtggaga gcaaacacgt catctccata gaagagcaat gtctccccac
     1741 acgaacagtc cgatcaagag gaagaatcgc ctcctccaga ccccgcaagc ttcagaaggc
     1801 cgagctttgt tggagcgcat gaccatggcg ccgatgtcgg cgagagacca cgcgtacgac
     1861 aagaatcaga aaatcaaccg aattttgttc cgactcaccc tgcgctatcc aacaattaat
     1921 actgttgtgt cataggccaa ctcagcgcca ttcaaggcat gatactccca taattccggc
     1981 cacgcgtgct cagcgtaacg ctgaccccta ctctgcttac ttttcaagtg taaatccact
     2041 atagctaagc ccaggattac tgatagacag agggggttgt actctggaag aatagggcat
     2101 gaggtgtt
//
LOCUS       DATMTI010000036         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_298610, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000036 DATMTI010000000
VERSION     DATMTI010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298610"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1089
                     /locus_tag="VN039_00620"
     CDS             148..1089
                     /locus_tag="VN039_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV44525.1"
                     /translation="MMDIQYQGRHPLLRIIRPPFFTPPIEKLGNFIINAYQSGRHGCR
                     VISFTRYGKTYAITYLKDNAGWRTDPAVFLDMRILAPEKCTERSFFTTMSLALHLREL
                     HQSSSAHAVMRVAHHLNILAEEEGAELIIFALDESQRLNGDDWQHVVTLDNEVSALGK
                     RLFLIFIHQLDTKGKEAESIHDDVPPQVLERFTKPCFDFPGIIGALEVKTCFEEYDAG
                     ATWPLGSGTTYSAYFAPDAFARGWRLSQHAQQVYDIATELRAESGLYTEGWQWPMVSL
                     EALMRYLLVSVPSRTPGFDGFTDEDIRKALIAAFFVD"
     gene            1497..2522
                     /locus_tag="VN039_00625"
     CDS             1497..2522
                     /locus_tag="VN039_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44526.1"
                     /translation="MLFHLPWVDRCPWHGARIIGQCPSCSRPLLPQFIDADLVPCDCP
                     CGVNFFDVKSAIHGDPSIDHHARAWQAIYLEWANEARTQRYLVTGADLQDLDAAEVGV
                     LVDLPLNLRTACCMHAAGAGKGRVQVLRPATSAAADQNATDFLLAMKALPVTERAAIN
                     VPPSLHRSFRAVAQNIVEKLPSRTFFEGDLLRIYPPDDPGHRRRPPGSPHRQHAELTF
                     LPLLQTSTGGVLDCTSIDKHIRSVVRGATWDLMEQLPHAQAGLIIRDLDLGTRTVLAA
                     LGKRLLLHGYAQGIRTILGRYVAALYDLPRDRPRPRRPIAIVFTYRDQVIKANLVWVK
                     APTPMDG"
BASE COUNT          535 a          760 c          679 g          587 t
ORIGIN      
        1 cgagcaaaag ttgcctgcca accactttct ccaggccggc acgccaatgg cgccccccaa
       61 agcgacgcct cgtaaacccg tcgcccagga tcccagcacg tacacgccga gtaccggctg
      121 ggtatcgttc cgctcaaaga ggggggcgtg atggacatcc aataccaagg tcgtcatccg
      181 ctcctgcgca ttatacgtcc gccgttcttt actccaccta ttgaaaagct cggcaacttc
      241 atcatcaacg cctaccaaag tggtcggcac ggctgtcgcg tgatcagttt taccaggtac
      301 ggcaagactt atgcgatcac gtacctcaag gacaatgcgg gttggcgaac cgaccccgca
      361 gtctttctgg acatgcgcat tcttgcgcca gaaaaatgca cggagaggtc gtttttcaca
      421 actatgtcgt tggcattgca cctaagggag cttcatcagt ccagcagcgc tcatgccgtg
      481 atgcgtgtcg cccatcacct caacatcttg gcagaagagg aaggtgccga gctcatcatc
      541 tttgccttgg acgaatcgca gcgtctgaat ggtgatgact ggcagcatgt agtgacgctg
      601 gacaacgaag tcagcgcatt gggtaagcgg ctttttctga tctttatcca tcagctggac
      661 acgaaaggta aggaggccga atcgattcat gacgatgtgc ctcctcaagt ccttgagcga
      721 ttcacgaagc cgtgctttga cttccccgga atcatcggag cgctcgaggt caagacatgc
      781 tttgaagagt acgacgcggg cgcaacctgg ccccttggaa gcggcactac ttacagcgct
      841 tacttcgctc ccgacgcgtt cgcgcgcgga tggcgccttt cccagcacgc acagcaggtc
      901 tacgacatcg ccaccgagct ccgcgcagaa agcggcttat acaccgaggg atggcaatgg
      961 cccatggttt cccttgaagc cctcatgcgc tacctcttgg tgtcggtgcc atctcgaacg
     1021 ccgggcttcg atggcttcac agacgaggat atccgcaagg ctctcattgc tgcgttcttc
     1081 gtcgactgaa tcggagaact ctcatgacca tccctcaggg ttatacgcct cgccaagcgc
     1141 acccgaacga acgatcattg cctgtcgcga tgcttgtagg atcgaatctt cgtcgctatc
     1201 cctacatgtc ggcttatggc gcgatgatgc gtgtgtgccg ccttcatgtg ttacctagag
     1261 aaatgcgatt tcgctattta ggtgttcgct ttaaccgcca tgatgatatc cccaaagcgt
     1321 tggcgacatc tggagtgcga aaagatcaac tttttcacca tatcggaggt gatgaaagca
     1381 taaggacata ctgggacatc aagccatggc tccccttctc cacgtccgta tcggaggctg
     1441 atctaatcac ccctctgcga atctgcccgt gctgcagtcg ctcgggctat cacaccatgc
     1501 tgtttcattt gccatgggtt gatcgatgcc catggcacgg tgcacgaatc atcggtcagt
     1561 gcccctcttg cagccgcccg ctgctacctc agttcatcga cgccgatctg gtgccatgtg
     1621 actgcccatg tggtgtcaac ttcttcgatg tcaaatctgc aatccatggg gatccttcga
     1681 tcgatcacca cgcccgtgca tggcaagcga tctatctgga atgggcgaac gaggcaagga
     1741 ctcagcgcta tctcgtcacc ggtgccgacc tccaggacct cgatgccgcc gaggtgggag
     1801 ttctggtcga tttgccattg aatctacgca ccgcatgctg catgcacgct gctggggctg
     1861 ggaaaggtcg cgtacaggtg cttcggccag cgacgtcagc agcagccgac cagaatgcaa
     1921 cggattttct gctggcgatg aaggcgttgc ccgtcaccga acgtgcggcc atcaatgtgc
     1981 caccgtcgct gcatcgatcc tttcgagccg tcgcgcagaa tatcgtagaa aaattacctt
     2041 cccgaacgtt tttcgagggg gacttgctgc gcatctatcc accagacgat cctggtcacc
     2101 ggcgtcggcc tcctggctcg cctcataggc agcatgcgga gttgaccttc cttccgttgc
     2161 ttcaaacctc cactggaggc gttctcgact gtacgtcgat cgataagcac attcgatccg
     2221 ttgtgcgcgg cgccacatgg gatcttatgg agcaacttcc tcatgctcag gccggactca
     2281 tcattcggga tcttgacctt gggacgagga cggttctggc ggcgctcggc aagcgacttc
     2341 ttctccatgg ttacgcacag gggattcgga ccattctagg ccgctatgtg gcagcgcttt
     2401 acgatttgcc gcgtgatcga ccaagacctc gtagaccaat tgccatcgtc ttcacttatc
     2461 gagatcaagt gatcaaggcg aatctggttt gggttaaagc gccgacgccg atggatggct
     2521 aatcccacaa ctaaccccgg ttttgagtgc agctagccgc c
//
LOCUS       DATMTI010000037         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_213833, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000037 DATMTI010000000
VERSION     DATMTI010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213833"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VN039_00630"
     CDS             complement(<1..779)
                     /locus_tag="VN039_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HWV44527.1"
                     /translation="MCGIIGYVGNQDAVPILIGGLAKLEYRGYDSSGVAVMQEEKIAV
                     RRSVGKLVNLQNSLKTNDLKGTVGIGHTRWATHGKPSEQNAHPHRSKGCVLVHNGIIE
                     NYQELKQQLQKDGYKFESETDTEVVAHLIDKYLQKENKLADAVRLATKDVRGSYALAV
                     ISEREPGTLIAARSGCPLVVGRTKHASYVASDVMAMLAHTREVTYLEEGDVAVVTQHR
                     VDLTDVDGHAVSRKSSTITWDASAAEKSGYPHFMLKEIHEQP"
     gene            complement(834..2435)
                     /gene="glmU"
                     /locus_tag="VN039_00635"
     CDS             complement(834..2435)
                     /gene="glmU"
                     /locus_tag="VN039_00635"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWV44528.1"
                     /translation="MKQSVEQQATASHIPGLGVIVMAAGLGKRMKSNQVKVLHHVAGQ
                     PMVLYAVDVALQLAGHRIAVVVGHQSDRVRQVIEVDIAGRPGSKSVRIVEQAEQLGTG
                     HAVMQSRPAFCQDDEPSPTNYLILNGDTPLLKEETVRELLRAHLMRSATVTILTAILD
                     DPSGYGRVIRRQSGGDHQGVITPSDVLKVVEDRDATPVERATQEINVGTYVVSGEFLF
                     DALDKLEPRNAQNEYYLTDIVRMAVAQERPVAAVTLQDPEEGLGVNTRQQLAAAEQVV
                     REQIRDRWLEAGVTMRDPGSVWIDAGVTIGRDTVLYPHVSLEGKTSVGEGTTIRSGVR
                     ISDCMIGNHVEILDHCVLRESQIDDDAHLGPFAHLRPGAILRRKAKVGNFVEMKKAEL
                     GEGSKASHLTYLGDARIGKGVNIGAGTITVNYDGGVKKHQTVIDDYAFVGSDSQIIAP
                     ITIGQGAIIAAGTTITQNVPADSLAIARVPQVNRVGWAAKRRMLLTTGTPSENSAKVS
                     DKQGGQSAKKTSSNMKNRRTKSLKR"
     gene            complement(2484..>3098)
                     /locus_tag="VN039_00640"
     CDS             complement(2484..>3098)
                     /locus_tag="VN039_00640"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HWV44529.1"
                     /translation="PIWGTILVLKTLFVAVDGILGDAVAELVPDHYIPGLGIVTLVLL
                     IFLVGLFAANFIGRQIVGHWEDWLNRLPLVRGIYSTLKSMMDILSFSERGSYRRVVLI
                     QFPKNGHYCFAFVTGMTKGETTALGQEALVHVYVPTSPNPTSGYFLLVPEREVTSVDI
                     SIEEAMKLIVSGGLYTPSGTMASALNGDSKWSQVKQPDAGVPIG"
BASE COUNT          645 a          945 c          782 g          726 t
ORIGIN      
        1 ggctgttcgt gaatctcttt cagcatgaag tggggatagc cgcttttctc cgctgccgag
       61 gcatcccatg taatcgtcga cgacttacgc gagacggcat ggccgtcgac atcggtgagg
      121 tccactcggt gttgcgtgac caccgccacg tccccctcct caaggtaggt cacttcccgc
      181 gtatgcgcga gcatcgccat cacatcggaa gcgacgtagg acgcgtgctt cgtccgaccg
      241 acgacgagcg ggcagccgga ccgcgcggca atcaacgttc cgggctcccg ctcggaaatc
      301 acagccagcg cataactgcc ccgcacgtct ttcgtcgcga gcctcacggc atcagccagc
      361 ttgttttctt tctgaagata cttgtcgatc aaatgggcga cgacctccgt atcggtttcc
      421 gattcgaatt tgtagccgtc tttttgcaac tgctgcttca gctcctgata attttcgatg
      481 atcccgttgt gcaccaacac gcatcccttt gatcgatgcg gatgggcatt ctgctcagat
      541 ggcttcccat gggtcgccca ccgagtatga ccgattccaa ccgttccctt gaggtcgttg
      601 gtcttgagcg aattttgaag gttgacgagt ttgcccacgc tccgtctgac ggcaatcttt
      661 tcttcctgca tgacggcgac tcccgaggag tcgtaaccac ggtactccaa cttcgccagc
      721 cccccgatga gaatcggaac tgcatcttga ttgccgacat atccgatgat tccacacatg
      781 acggttgctc caggtctaac gaggctgatc ggcagacgat tctcccgccg aaatcagcgt
      841 ttcagtgact tcgtgcgcct gttcttcatg ttgctcgaag tctttttggc gctttgacca
      901 ccctgcttgt cggaaacttt cgctgaattt tcgctcggtg taccggtggt cagcagcatc
      961 cgacgtttag ctgcccaccc gactcgattg acctgcggca cacgggcaat cgccaaggag
     1021 tcggccggca cattttgtgt gatggtcgta ccggctgcaa tgattgctcc ttgtccaatg
     1081 gtgatcggtg cgatgatctg ggaatcgctt ccaacaaacg cataatcgtc gatcaccgtc
     1141 tgatgcttct tcaccccccc gtcataattt accgtgatcg ttcccgctcc aatattgacg
     1201 cctttcccaa tccgcgcatc gccgagatat gtaagatgac tcgccttgga tccttcgccg
     1261 agttcggcct tcttcatttc cacaaaattc ccgaccttcg ccttccgccg caggatggcc
     1321 ccaggtctca aatgtgcaaa tggtccaaga tgagcgtcgt catcgatttg cgattcgcgc
     1381 agcacgcaat gatcgagaat ctctacatgg tttccgatca tgcaatccga aatgcgcacc
     1441 cctgaacgga ttgtcgttcc ttcaccgacg cttgttttgc cttcgaggct cacgtgtgga
     1501 taaagcacgg tatctcttcc gatggtgacc ccggcatcaa tccacacgga gccaggatct
     1561 cgcattgtga ctccagcttc aagccagcga tcgcgaatct gctcacggac gacctgttcc
     1621 gcagccgcca gctgttgcct ggtattgact cccagtccct cttcgggatc ttgaagggtc
     1681 accgccgcaa ccggccgctc ctgtgcaacg gccattcgaa caatatcagt cagatagtat
     1741 tcattctgtg cgttgcgtgg ttccagctta tcgagggcat caaagagaaa ttcgccggac
     1801 acgacatatg tcccgacgtt gatttcctgc gtggcccgtt ctaccggagt ggcatcacga
     1861 tcttcgacga ccttcagcac gtcggatgga gttattacac cttggtgatc gccgccggac
     1921 tgtcggcgga tgactcggcc atagccgctg ggatcatcga gtatcgccgt cagaattgtc
     1981 acggtcgcgc tccgcattag atgggctctc aaaagttcgc gcaccgtctc ttccttcagc
     2041 aacggagtat caccgttcaa gatgagataa ttcgtcggac tcggctcgtc gtcttggcaa
     2101 aacgcggggc ggctttgcat cacggcatgg ccggtcccca gttgctcggc ttgttcgacg
     2161 atacgcaccg actttgatcc aggcctgccc gcaatatcca cttcaatcac ttgccgaact
     2221 ctgtcggatt gatgcccgac caccaccgca atgcgatggc ccgccagttg tagtgccaca
     2281 tcaacagcgt agagaaccat gggctgcccc gcgacatgat gcagcacctt tacttggttg
     2341 gatttcatcc gcttgcccaa tccagccgcc atcacgatga ctcctaagcc gggaatatgc
     2401 gaggccgtcg cctgttgctc caccgattgt ttcatcgagg actccggata tgagaggatc
     2461 gcaatcgagt atacggcacg tatttatccg atcggcacac cggcatcggg ctgtttgacc
     2521 tgactccact tcgagtcccc gttcagcgcc gaagccatgg tgccggacgg cgtatagaga
     2581 ccgcctgaca cgatcagttt catcgcttcc tcaatactga tatcaacaga tgtcacttcc
     2641 cgttccggca ccaatagaaa atagcccgac gtcggattgg gcgaggtggg tacgtagaca
     2701 tgaaccagcg cctcctggcc caatgccgtt gtctcccctt tcgtcatgcc ggtcacgaac
     2761 gcaaaacaat aatggccgtt cttcggaaat tggatcaaga cgacacgccg atacgatcct
     2821 cgctccgaaa aggatagaat atccatcatg gatttcaacg tggaataaat tccccggacg
     2881 agaggcagtc ggttaagcca atcctcccag tgacccacaa tctgacgccc gatgaagttc
     2941 gccgcgaaca acccgaccaa aaaaatgagc agcaccaggg tgacgatccc taggccgggg
     3001 atataatggt cgggcaccaa ttccgcgacg gcgtcgccta gaatgccgtc taccgccacg
     3061 aacagggtct tcaggacgag aatagtgccc cagatcgg
//
LOCUS       DATMTI010000038         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_256222, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000038 DATMTI010000000
VERSION     DATMTI010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256222"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..610
                     /gene="rimP"
                     /locus_tag="VN039_00645"
     CDS             104..610
                     /gene="rimP"
                     /locus_tag="VN039_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWV44530.1"
                     /translation="MSKGNGLSIPGNGPQPVADRLREVISPILWTLGLELVDVVCVGQ
                     GPRSIVRVLIDKSGGVSIADCEQAHKALGPALDVADPFPHAYTLEVSSPGLDRPFKRS
                     QDYQRAIGKEVSLKLRQPLEGQWRITGQLMQVDEQAVVLTVGATRTPQTVKLDRDMIA
                     EAKLVVKI"
     gene            663..1814
                     /gene="nusA"
                     /locus_tag="VN039_00650"
     CDS             663..1814
                     /gene="nusA"
                     /locus_tag="VN039_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWV44531.1"
                     /translation="MNRELISVIDEIGRQKGIDKARVIGAIESALQTAAKKRFGQAEN
                     IQVEIDPKTGEISVVSKKIIVETVGNPKAEISLQEARQYDSEAEIGDEIGSLIEMNEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVTGIILGMERRNYLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKAMLLEVRRTPKDVQVILSRSHPQFVAKLFELEVPEVMEK
                     IIEIRSVVREPGDRTKIAVTSREKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     QDPRVFIAEALNPATIEKVGIDEEKKSALVVAADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKVERDKEIKAALAEEAEAQRLQEEARQAVRAEEATSG"
     gene            1830..>2382
                     /locus_tag="VN039_00655"
     CDS             1830..>2382
                     /locus_tag="VN039_00655"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HWV44532.1"
                     /translation="MAMRVYELAKKLGMENRVLIPELKKMGASVSSHSSTLDDEIVQK
                     VLDKLGSKAKGQGTEIEGEPGAKSGEHASHGKTMAARSHVVDEPSKPDKRRILIKRKK
                     EDEPTEVISSAPIVEGERQVQPTPSITVPTPPSSTEHPGGGDLVDRNLPAEESSNEIS
                     PPAPAPIVAKQEEVPAKPTVASPT"
BASE COUNT          609 a          578 c          742 g          453 t
ORIGIN      
        1 cgctgggcat tgttgaccgg ctagtcccct tgctttgcat aggagtgcat ggtatggtgc
       61 cccttctgta agtgggctca tgcccacttt tttgtttgga cacatgtcaa agggaaatgg
      121 gttgagcata ccgggcaatg gtccgcagcc ggttgccgac cggttacgcg aggtcatttc
      181 gccgattctt tggacgctcg ggcttgagtt ggttgatgtg gtgtgcgtgg ggcaaggacc
      241 ccgctcgatc gttcgtgttc tgatcgataa atctggtgga gtcagcatag cagactgtga
      301 gcaggcacat aaggctctag ggccggcgct cgacgtggcc gatccgttcc cccatgccta
      361 cacccttgaa gtctcttctc cgggtcttga tcgacccttc aagagatctc aggactatca
      421 acgggcaatt ggaaaagagg tgagcctcaa acttcggcaa ccgctcgaag gccagtggag
      481 gattaccggg cagctgatgc aggtggatga gcaggcggtc gttctgaccg tgggcgcgac
      541 gcggacacct cagacggtga aactggatcg agacatgatt gctgaggcga agctggtcgt
      601 gaaaatttag gggctggaga gttggagatt gtaagcggta accggatgca ggagcgtgac
      661 gtatgaaccg agaactgatc tcagtgatcg acgaaatcgg gcgtcagaaa ggaattgaca
      721 aggcccgagt catcggggcc attgaatccg ccctgcagac ggccgcgaag aaacgcttcg
      781 gccaggccga aaacatccaa gtggagatcg acccaaaaac cggggaaatt tccgtcgttt
      841 ccaagaaaat catcgtcgaa acagtcggca atcccaaagc ggagatctct cttcaagaag
      901 ctcgccagta tgatagtgaa gcggagatcg gcgacgaaat cggatcgctg atcgaaatga
      961 acgaattggg gaggattgcc gcacaaacgg caaaacaagt catcttccag aaggtgcgtg
     1021 aggcggaatg ggaagcggtt caaaaagaat attcgacgcg tcagggggat ctcgttacgg
     1081 gaatcattct cggcatggaa cgccggaatt acctggtgga cctcggaaag acagaggcca
     1141 tcctgccgat ccaagagcag attccgcgtg aaacatatcg gcgcggcgat cgtgtgaaag
     1201 cgatgttgct ggaagttcgt cgtacgccga aagatgtgca agtcattctg tcccgtagcc
     1261 atccgcagtt cgtggcgaag ctttttgaac ttgaagtgcc ggaagtcatg gaaaagatca
     1321 tcgaaatcag gtccgtcgtt cgagagccgg gagatcggac gaagatcgca gtcacgtcac
     1381 gtgaaaaggc cgtggatccg gtgggggctt gtgtcggtat taaaggctcc cgcgtgcagg
     1441 ccgtcgttcg cgagttgcgg ggcgagaaga ttgatatcat tacctggacc caggacccgc
     1501 gggtctttat tgcagaagcc ttgaaccctg cgacgatcga aaaggtagga atcgacgaag
     1561 agaagaagtc ggcgctcgtg gttgcagccg actcgcaact gtcgttggcg atcggcaaaa
     1621 acggtcagaa tgtccggctt gccgcgcgtt tgacgggatg gaagatcgat atcatcagcg
     1681 caacggaata cgaaaaggaa aaagtagaac gcgataagga aattaaagcg gcgctcgcag
     1741 aggaagccga ggcgcaacga ctgcaggaag aagctcggca ggctgtcaga gccgaggaag
     1801 caacgagcgg atagaaacgg aactgtctca tggctatgcg cgtatacgaa ctcgcgaaga
     1861 agttagggat ggaaaatcga gtgctcatcc ccgagctcaa gaagatgggg gcatcggttt
     1921 cctctcatag cagtacgctc gacgatgaaa tcgttcagaa ggtattggat aagctgggct
     1981 cgaaagcgaa aggtcagggg acggaaatcg aaggagagcc cggtgcaaag tcaggggagc
     2041 atgcaagtca cggcaaaacc atggcggcaa ggagtcacgt ggtcgatgaa ccgtccaagc
     2101 ctgacaagcg tcgtattctg atcaagcgaa agaaagaaga tgaaccgacc gaggtcattt
     2161 cttcagctcc aatcgtcgag ggtgagcgtc aggtgcagcc gacaccctca atcaccgtgc
     2221 cgacaccgcc ctcatccact gagcatcccg gcggcggtga cctcgtcgat cgcaatcttc
     2281 cagcggaaga aagctcaaac gagatttctc cacccgcacc ggcgccgatc gttgcgaaac
     2341 aggaggaggt gccggcaaag ccgaccgtcg cttcgccgac ga
//
LOCUS       DATMTI010000039         1759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_44099, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000039 DATMTI010000000
VERSION     DATMTI010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44099"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..865)
                     /locus_tag="VN039_00660"
     CDS             complement(68..865)
                     /locus_tag="VN039_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWV44533.1"
                     /translation="MRPVDEALVKAALTGDLNEVHALIDKGADVKATTEEGYTALYAA
                     AQSNHREIVVLLLERGADVGHTTSSGTTALLIAAQKGNLEIVTLLLANGANVGQATPK
                     GATPLYIAAQNGHREVVALLLEKGASVNQADNEGVSPLMIAARYGHREVVALLLQQKG
                     AVNQITQDGRTSLYLATLNGHQDVVALLLDKGAVTSQADQDGATPLHLAAQNGHRDIA
                     ALIVEKGADMKARTKEEGSTTLMIASQNGHHGVVALLLKKEADIAKR"
     gene            complement(1046..1119)
                     /locus_tag="VN039_00665"
     tRNA            complement(1046..1119)
                     /locus_tag="VN039_00665"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1085..1087),aa:Gln,seq:ttg)
     gene            complement(1166..1239)
                     /locus_tag="VN039_00670"
     tRNA            complement(1166..1239)
                     /locus_tag="VN039_00670"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1205..1207),aa:Gln,seq:ctg)
     gene            complement(1309..>1759)
                     /locus_tag="VN039_00675"
     CDS             complement(1309..>1759)
                     /locus_tag="VN039_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWV44534.1"
                     /translation="LVPLLEQAGLRKEVEAVSDEWLAQLVVLVKERAKTLVDMVEWVR
                     PYFGQEVQFEAEAAKKFLTPTVAPLLHKLLTRFEAFPSFSKPIWDESFKKLVEEEGIK
                     MGALAQPVRVALTGRTASPGLFEVMEVLGRDRTLFRLQKGLERAAMA"
BASE COUNT          418 a          524 c          419 g          398 t
ORIGIN      
        1 gcttcaataa cggcagtcat caacagctgc tcacgcagct taaatactgg tacaaatcaa
       61 ccaaacatta gcgcttagct atgtcagcct ctttttttaa gaggagagcc acgactccat
      121 gatgcccgtt ctgggacgcg atcatcaatg tggtcgaccc ttcctcctta gtcctcgcct
      181 tcatgtcagc gcccttctcc actatcaacg ctgcgatatc acgatggcca ttctgagcag
      241 caagatggag cggcgtcgca ccgtcctgat ctgcctggct tgttactgca cccttgtcta
      301 gcaaaagcgc tacgacatct tgatgcccgt tgagagttgc aagataaagc gaagtcctac
      361 catcttgcgt tatctgattc actgctccct tctgctgcag tagaagtgct accacctctc
      421 gatgtccata ccttgccgct atcatgagcg gcgatacgcc ctcattgtca gcttggttta
      481 cagatgctcc cttttccaag aggagagcga caacttcacg gtgcccgttc tgggccgcaa
      541 tgtagagagg tgtcgcccct ttcggcgtgg cctgtccaac gttggcccca ttggccaaca
      601 gcaatgtcac aatttccaga ttccctttct gagccgcaat aagaagtgct gttgtaccgc
      661 tcgacgttgt gtgtcccacg tccgcacccc tttccaatag caacaccaca atttcacgat
      721 ggttactctg tgccgcggca taaagagcgg tataaccttc ttcagtggtc gccttgacgt
      781 cagcgccctt gtcaataagt gcatggacct cgttgagatc acccgtgagc gctgctttga
      841 cgagcgcctc gtccactggc cgcatagcta agcaacattg gcattagccc aagtatcgct
      901 atcaagtttg gccatgttcg cctcatcgca atcctcctca gactaggtgg cagagcaatt
      961 gcgcgcgagg atacaaaacc cgtggattcc aagcaacaga aattactgag aatcagcgga
     1021 gaaaaatggc gggacagtaa gataatggct gggggactag gaatcgaacc taggtagccg
     1081 gctccaaagg ccggcgtcct accgctagac gatcccccag cgcggtacgg aagagaacac
     1141 tgacgataag gctggaaaga taaattggct gggggactag gaatcgaacc taggtagtca
     1201 gatccagaga ctgacgtcct accgctagac gatcccccaa ccaaggttca cagtaatata
     1261 tcaatcgcaa ttccgtcaag atcgatgcgg gtttggcagg atgacccttc atgccatggc
     1321 ggcgcgctca agcccttttt ggagacgaaa caaggtccgg tcacgaccca gtacttccat
     1381 cacctcaaaa agtcccggac ttgccgttcg tccggtcaag gcgacgcgta ccggctgggc
     1441 taatgcaccc atcttgatgc cttcttcctc gaccaacttc ttgaaactct catcccaaat
     1501 cggtttggaa aagctgggaa acgcctcgaa gcgcgtgaga agcttgtgca gcaatggagc
     1561 gaccgtggga gtgagaaatt tctttgctgc ctccgcttca aattggacct cctgcccaaa
     1621 ataaggcctg acccactcca ccatgtcgac caatgtcttg gcccgctcct tcaccaggac
     1681 caccagctgc gcaagccatt catccgaaac ggcctcgact tccttccgca accccgcttg
     1741 ctccagcaac ggcacaagc
//
LOCUS       DATMTI010000040         5649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_722994, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000040 DATMTI010000000
VERSION     DATMTI010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722994"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VN039_00680"
     CDS             <1..487
                     /locus_tag="VN039_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWV44535.1"
                     /translation="PVVAGIFNPSTDELFTATRGGGLRLNGKTVTPQDTGGDRKPVVA
                     LSPRERKIRRFRSLEMSTKSHSIRSIAWALAVTASGRIHAAATLEPENEWDVAAGALL
                     ITEAGGAIYDGRGHDLTFNRREPCYSGIIATSPHCPDTLTQQLKRLTRTGEDGVEQSF
                     S"
     gene            complement(491..1648)
                     /locus_tag="VN039_00685"
     CDS             complement(491..1648)
                     /locus_tag="VN039_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWV44536.1"
                     /translation="MRTVSRRMVDLAQSEIRAMTQACASVKGLNMAQGVCDTPVPPVV
                     LEGAEKAIRDGHNVYTRFDGVLELRQAIADKLAHYNGIRADPETEVTVSAGATGAFHC
                     ACEALLDPGDEVILFEPYYQYHISALRAVEAVAVIVKMRAPKWTFDSAQLEETVTPKT
                     KAIIVNSPGNPSGKIFSRTELEVLADFASRHDLFVMTDEIYEYFLYDGRRHVSMASLP
                     NMAERTVTIGGYSKTFSVTGWRIGYSMAAQPWTRAIGAMNDLLYVCAAAPLQMGVARG
                     IRELSENFYVSLARDYQQKRDKFCSTLAKVGLAPSIPEGAYYVLADVSRLPGDTGKDR
                     AMYLLEKTGVAGVPGEAFFPGAAGANYIRFSYAKTDADLDEACRRLTQAIF"
     gene            complement(1883..2497)
                     /gene="msrA"
                     /locus_tag="VN039_00690"
     CDS             complement(1883..2497)
                     /gene="msrA"
                     /locus_tag="VN039_00690"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWV44537.1"
                     /translation="MLRRLAFLAIGLLTCATFALWPSAGLVKASTQAKAYFAGGCFWC
                     MEEAFEKVDGVTAVASGYMGGTVANPTYEQVSDGHTGHAESIEVTYDPSKVTYQKLLD
                     AFWRNIDPVTPNAQFCDHGAQYRAVVFYSTDEEKRLPEEWKSKIEQSKRLPAPIVTQL
                     VQASTFYQAEDYHQDYYKKNPLRYKYYKYSCGRAQRLEALWGKP"
     gene            complement(2593..3135)
                     /locus_tag="VN039_00695"
     CDS             complement(2593..3135)
                     /locus_tag="VN039_00695"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44538.1"
                     /translation="MRFVVGVMGPAKASKRDLDNARVLGEFIARRGWVVLTGGRDVGV
                     MDAACEGAKRVGGSLTIGVLPTAKDKVSRHVDVPIITEMGSGRNNINVLTSHVVVVCG
                     LSGSGTVSEVALAVKAGKPVILVEASPADVAFFRKLDQRLVNAAASPEEAIELIMKLM
                     DGPQRRAHRADREPFPYLPV"
     gene            complement(3166..4803)
                     /locus_tag="VN039_00700"
     CDS             complement(3166..4803)
                     /locus_tag="VN039_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44539.1"
                     /translation="MSIRRLLLWAVSWLGLLAIDPSTAAERHVSRVPLFDNLGTLHHP
                     ITTNSHEAQQYFDQGLRLVYAFNHEEAVMAFEEAAHLDPSAAMAYWGVALASGPNINA
                     AMDKAAERRAWDAVQKARSHRAHASPAEQAYIDAIGKRYDVNAHARPALDKAYADAMR
                     VVWRQFPNDPDAGVLFAEALMDLRPWDLWTTAGRPKPGTEELVSTLESLLTRFPDHPG
                     ACHYYIHSMETSLTPDRAVACAERLPGLMPGAGHLVHMPAHIYMRLGRYHEAAERNAH
                     AADVDRNYLAGRKLSGNYADAYYTHNLHFLWASLAMEGRRTEALKVARRLTGTITEDE
                     ARKDKWKELYLPTPLWSMIRFGQWDELLREPPPPKPLRLQQGIWRLGRGMALSASGRL
                     PGAEGEHLVLTGMAKRLGRDRTPEDKTERTMLKIAERLLAGELAMHRHKYDEGITSFT
                     DAVKLEEELPYTEPPFWPIPIRHYLGAALLRAGQPGKAEIVYRADLAKNPRNGWAQFG
                     LMQSLRAQRKGREADVEEKQWQQAWEHADVTLTASRF"
     gene            complement(4961..>5649)
                     /locus_tag="VN039_00705"
     CDS             complement(4961..>5649)
                     /locus_tag="VN039_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44540.1"
                     /translation="VQMKGIRQMAPRFNKEPINLIHRIPSQKQIDEARPVPAPYGQLL
                     LDAGAACWRKVVDLFAYVTRYEDEAHQRVRKPLALPDRTMEHATIHHDDIQQNGKRVQ
                     EAESQSATKVTILRKPVQAPTLVQSEEVAELRSFLVGQQDEIARLTSHIQELTSLVSS
                     QQQVLVHLGKELEVGAFSTTSNGTAAASIKRSRIGRRKPTDNETPNPSQAQENEHPFS
                     PQTEHRSLNL"
BASE COUNT         1150 a         1671 c         1564 g         1264 t
ORIGIN      
        1 gcctgtcgtc gcaggtatct tcaatccttc caccgatgaa ttgttcaccg caacccgcgg
       61 tggcggcctt cgtctcaacg gcaagacggt tacgccgcaa gatacgggcg gggaccggaa
      121 accggttgtt gccttgagtc cgcgggaacg caaaatccgc cgctttaggt cgcttgagat
      181 gtccaccaaa agccactcga ttcgttccat cgcctgggcg cttgccgtca ccgcgagcgg
      241 acggatccac gcggcggcga cgctcgaacc ggagaatgag tgggatgttg cggccggagc
      301 tctcctcatc acagaagcag gcggggcaat ctatgacggg cgcgggcatg atctgacgtt
      361 taaccggcgt gagccttgct atagcgggat catcgccact agtccacatt gtcccgatac
      421 tctcacgcag caactcaagc gtttgactcg cacgggcgag gacggtgttg aacaatcgtt
      481 cagctgattg ttagaagatg gcttgagtca agcgccgaca ggcttcatca agatcagcat
      541 ccgtctttgc gtaactgaag cgaatatagt tggctcctgc cgcaccgggg aaaaaggcct
      601 ctcccggtac accggccaca ccggttttct ctaacagata catggcacga tctttccctg
      661 tgtcgcctgg aagccgggac acatccgcca acacgtaata ggctccttct gggatcgacg
      721 gagcgagtcc caccttggcc agagtgctgc aaaatttgtc acgcttttgc tgatagtctc
      781 gagccagact tacatagaag ttctcggaca attctcggat accacgagcc acgcccattt
      841 ggagcggcgc cgcagcacag acgtagagca aatcattcat cgccccgatg gctcttgtcc
      901 acggctgtgc agccatgcta tacccgatcc gccatcctgt cacgctgaac gtcttggagt
      961 accctccgat ggtcaccgtc cgttccgcca tattcggcaa ggaggccatg ctcacatgtc
     1021 gccgcccgtc atagaggaag tattcgtaga tttcatccgt catgacgaat agatcgtgcc
     1081 gggacgcaaa atctgctagc acctcaagtt ccgttcggct gaatatcttc cctgatgggt
     1141 tgcctggcga attgacgatg atggccttgg tcttaggcgt gaccgtctct tcgagctgag
     1201 ccgagtcgaa cgtccatttc ggagccctca tcttcacaat caccgccact gcctcgactg
     1261 cgcgcaaggc actgatgtgg tactgatagt agggttcgaa gagaatgact tcgtcacccg
     1321 gatcgagcaa cgcctcacac gcgcagtgaa aggcgcctgt tgctccggcg ctgacggtca
     1381 cttccgtttc aggatcagct cgaatcccat tatagtgcgc cagcttgtcg gcgatagcct
     1441 gccgtaattc aagcacgccg tcgaagcggg tgtagacatt gtgcccatcc ctgatggcct
     1501 tctctgcccc ttcgagaacg accggcggca ccggcgtatc acagacaccc tgtgccatgt
     1561 tgaggccctt cacgctcgca caagcctgtg tcatcgcccg aatttccgat tgggcgagat
     1621 ccaccatccg ccgactgacc gttcgcatca ccatactcct tcgtaaccca gcctagatcg
     1681 gtgtcctttg tggcagagga ttgtgccagg gtcaaggggg gcacaatgtc gagtgcgatg
     1741 gaaagttgta catgccaact gtaaaggggg tggcgaccgt gcaggatgtg ccgatgaacg
     1801 gctgtggagt tgaatcatcg gtcgaaatgg agattgggat agcctcgaaa tttcccttgt
     1861 tccttgctca ctcctgttac tcctacggct tcccccataa cgcttccaac cgttgggctc
     1921 ggccgcagct gtatttgtaa tacttatacc tgagtgggtt cttcttgtag taatcctggt
     1981 gatagtcctc agcctggtag aaggtggacg cttggactaa ctgcgtcacg atgggggcag
     2041 gaagccgctt ggactgttcg atcttgcttt tccattcttc cggaagccgt ttttcttcat
     2101 cggtagaata gaagaccacc gcgcggtact gagccccatg gtcgcagaat tgcgcgttcg
     2161 gcgtgaccgg atcgatgtta cgccaaaagg cgtccaatag tttttggtac gtgactttcg
     2221 acggatcata ggtcacttca atagactcgg cgtggcccgt gtgtccgtct gagacctgtt
     2281 cataggtggg attggcgacc gtgcctccca tgtagccgga tgcgaccgcg gtaacaccat
     2341 ctactttttc gaatgcctcc tccatacacc agaagcaacc gcccgcaaag taggctttcg
     2401 cctgtgtcga tgccttcacc aatccagctg aaggccaaag agcgaatgtc gcacaggtca
     2461 gcaaaccgat cgcgaggaac gccagtcgtc gaagcatcgc atgatcctcc ttctccatcg
     2521 tcgtcccagt gaaccaattc ttacatctta ctacgacgat cgctgagagg gtgggcgtgt
     2581 atcattcgat gttcacaccg gcagataagg aaatggctcc cggtcggcgc gatgcgccct
     2641 ccgttgaggc ccatccatga gcttcatgat cagctcaatg gcttcttccg gtgacgcagc
     2701 ggcattaacc aatcgttggt cgagtttgcg aaagaacgcg acgtcggcgg gagaggcttc
     2761 aacaagaatg accggtttac ccgccttcac ggccaaggcg acctcggaca ccgtccctga
     2821 tccgctcaat ccgcacacca cgacgacatg gctggtcaag acattgatgt tgttacggcc
     2881 actccccatt tccgtgatga tcggcacatc cacatgacga gacaccttgt ccttggccgt
     2941 cggcaagacc ccgatagtca agcttccgcc cacccgtttg gccccttcgc aagccgcgtc
     3001 catgacgccc acgtcacgac cgccggtcaa tacgacccaa ccacgccggg caatgaattc
     3061 tccaaggacc cgcgcattgt cgaggtcccg cttcgaggct ttggccggcc ccatcacccc
     3121 aaccacaaat cgcatcgctt cctcctgcag tccgtttccg ttccttcaaa accgagaggc
     3181 agtgagggtc acgtccgcat gctcccatgc ctgttgccat tgcttttcct cgacgtccgc
     3241 ttcgcggcct ttccgctgag cacggagact ctgcatgagt ccaaactgag cccagccgtt
     3301 ccgcggattc ttcgccagat ccgctcggta aacgatttcc gccttgccgg gttgaccggc
     3361 tctgagtaag gcggcaccga gatagtggcg gatcggaatc ggccaaaatg gaggttccgt
     3421 gtaggggagc tcctcctcca gcttaacggc atcagtgaat gatgtgatgc cctcgtcgta
     3481 cttatggcgg tgcatcgcca gttccccggc cagcaatcgt tccgcgatct tgagcatagt
     3541 ccgttcggtt ttatcctccg gagtccgatc acggcctaag cgcttggcca tgccggttag
     3601 taccaaatgc tcgccctccg ctccgggcaa gcgacctgac gccgatagcg ccatgcctct
     3661 ccccagtcgc catatcccct gttgcagacg cagcggcttg ggaggaggcg gttcacgcag
     3721 cagttcgtcc cattgtccga acctgatcat cgaccacaag ggagtgggaa ggtacaattc
     3781 cttccattta tccttccgcg cttcgtcctc ggtgatcgtt cccgtcagtc ggcgtgccac
     3841 cttcaatgcc tcggtccttc gcccctccat cgctaaggaa gcccaaagaa aatgcagatt
     3901 gtgggtgtag taggcatcgg cgtagttgcc gctcaacttt cggccggcca aatagttgcg
     3961 atccacatcc gcagcatgcg cattccgttc agccgcttca tggtatcgac cgagcctcat
     4021 gtagatgtgg gccggcatgt ggactaagtg tccggccccc ggcataagcc cgggcagccg
     4081 ttccgcacaa gccacggctc ggtcaggggt cagtgaagtt tccatgctat ggatgtagta
     4141 gtggcaggcc cccggatgat ccgggaaacg cgtcagaagc gattccaatg tagacacgag
     4201 ctcttcggtg ccgggctttg ggcgaccagc cgtcgtccat aaatcccatg ggcgtaggtc
     4261 catcaaggcc tcagcgaaca agacgccggc atccggatcg ttcggaaact gccgccagac
     4321 tacacgcatg gcgtccgcgt aggctttatc gagggccggt cgcgcatgcg cgttgacgtc
     4381 gtatcgctta ccgatcgcgt cgatataggc ctgctcagct gggctggcat gggcacgatg
     4441 tgagcgagcc ttttgtacgg catcccatgc ccgccgttct gctgctttat ccattgccgc
     4501 gttgatgttg ggacccgagg cgagcgccac accccaatag gccatcgccg ctgagggatc
     4561 cagatgcgcg gcttcttcaa aggccatgac cgcctcttca tggttgaagg catacacgag
     4621 ccgtaaacct tgatcaaagt attgttgagc ctcgtgggag ttcgtcgtga tgggatgatg
     4681 aagggtgccg aggttgtcaa aaagaggcac ccgagagaca tggcgttcag ctgccgtact
     4741 cggatcgatc gccaacagac ctagccaaga caccgcccac aagaggaggc gacggatgct
     4801 caaggacggc tttgtagcag acgtgaccag cacctggcta gagcaaaggt gtacgtgttg
     4861 aggggatcac tctcgcgggt agtaggattc gctcttttta cctgcgaagt gttcaaaaag
     4921 cggctggagc caccgaccaa cactgcagat cgggcctagc ttacagattg agcgaccggt
     4981 gttcagtttg aggcgaaaac ggatgctcat tttcctgagc ctgagaagga ttcggcgtct
     5041 cgttgtcagt cggtttcctc cgaccgatac ggcttcgctt gatagaggca gccgccgtgc
     5101 cgttcgaggt cgttgaaaat gcgccgactt ccagctcttt gccgagatga acgaggacct
     5161 gttgctgaga gctgaccaag gaggtgagct cttgaatgtg tgaagtcagc cgagcaatct
     5221 catcctgttg cccgaccaga aaggaccgga gttcggcgac ttcttccgac tgaacaagag
     5281 tcggtgcctg gaccggcttt cgtaggatcg tgacttttgt ggccgactga ctctctgctt
     5341 cctggactcg ctttccgttc tgctgaatgt catcatggtg gatcgtggca tgttccatcg
     5401 ttcggtcggg tagcgcaagc ggtttacgga cccgctgatg agcctcgtcc tcataccgcg
     5461 tcacgtaagc gaagagatca actaccttgc gccagcaagc ggctccggca tcaagcagga
     5521 gctgaccgta aggtgcgggc accggacgag cttcatcgat ctgcttctgc gacggaattc
     5581 gatgaatgag gttgatcggt tctttattga atcgtggggc catctgtcgg atccccttca
     5641 tttggacct
//
LOCUS       DATMTI010000041         1630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_426046, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000041 DATMTI010000000
VERSION     DATMTI010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426046"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VN039_00710"
     CDS             <1..408
                     /locus_tag="VN039_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWV44541.1"
                     /translation="EIAQNNQYVIHNPYNVENPDMTIVSWGSTKGVILDSIKVLKEKH
                     NLNVNFLQIRMMSPFPTEAVTDVLKKAKRAVVVESSFGGQITQLITMRTGIQIQHEVL
                     KWSGRPISETELVAAALEINQKQTRKVELSYGL"
     gene            398..1387
                     /locus_tag="VN039_00715"
     CDS             398..1387
                     /locus_tag="VN039_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWV44542.1"
                     /translation="MASSPSVLKAPLKGSQLKSDIHSEWCPGCGDFGITNSIQQAVAG
                     LGLKPWETFIVSGVGCSGKVPHYIHTYGMHTLHGRSLPFASGAKLSNPDLTIIAAGGD
                     GDGYGIGAGHFVHAGRRNIDMTYVVFNNEVYGLTKGQASPTLAQGAQPKSLALPNPTT
                     ALNPIALALSCGYTFIARSYAFDAKHLKETLMKAIQHKGMALVDVLQPCPTYNNIHTK
                     DYFAEMVEVKGSSVPRVYYLEDDGYDGKVKNPHDIHEINEKQAACFERAHKVEERVAL
                     GVYYQIEKPTFHDILKQNMPLLNEYTPIQLPYYDSQTMVANTDLAAAAADITI"
BASE COUNT          405 a          487 c          412 g          326 t
ORIGIN      
        1 gaaatcgcgc agaacaacca gtacgtcatc cacaacccgt acaatgtgga aaacccggat
       61 atgaccatcg tcagctgggg ctccaccaag ggcgtgattc tggacagcat caaggtgctg
      121 aaagagaagc acaacctgaa cgtcaacttc ctgcaaatcc gcatgatgag cccgttcccg
      181 actgaagccg ttaccgacgt gctgaagaag gccaagcgcg ccgttgtggt ggaaagcagc
      241 ttcggcggac agatcaccca gttgatcacc atgcgaaccg gcatccagat tcagcatgaa
      301 gtgctgaagt ggagtggtcg tccgatttcg gaaaccgaat tggtcgccgc cgccctcgaa
      361 attaaccaaa aacagaccag aaaggtggaa ttgtcctatg gcctctagtc ctagcgttct
      421 caaagcgcct ttaaaaggct cccaactaaa aagcgacatt cactccgagt ggtgtcccgg
      481 ctgtggtgac tttggtatca ccaactccat ccagcaagcc gtcgctggac tgggcttgaa
      541 gccttgggaa accttcatcg tttccggtgt gggttgctcc ggcaaagtgc cccactacat
      601 ccacacctac gggatgcaca ccctgcacgg tcgctccttg ccgttcgctt ccggtgccaa
      661 gctgtccaac ccggatctga ccatcatcgc cgccggtggt gacggtgacg gttacgggat
      721 tggcgcgggc cacttcgtac acgccggtcg tcgcaacatc gacatgacct acgtggtgtt
      781 caacaacgaa gtgtacggtc taaccaaagg ccaggcctcc ccgacattgg ctcaaggcgc
      841 tcagccgaag tccctggcgt tgcccaaccc caccacggcg ctcaacccga tcgccctggc
      901 gctgtcctgc ggctacacct tcatcgcccg cagctacgcc ttcgacgcca agcacctgaa
      961 agaaaccctg atgaaagcca tccagcataa agggatggcc ctggttgacg tgctgcaacc
     1021 ctgccctacc tacaacaaca ttcacaccaa agactacttt gctgagatgg ttgaagtcaa
     1081 aggcagctcg gtgccccgcg tttactatct ggaagacgac ggttacgatg gcaaggtgaa
     1141 aaacccccac gacatccacg agatcaacga aaagcaggcc gcttgcttcg agcgcgccca
     1201 caaagtcgaa gagcgcgtgg ccctgggtgt ttactaccaa atcgaaaaac ccaccttcca
     1261 cgatatcctg aagcaaaaca tgccgttgct gaacgagtac acgcctatcc agttgccgta
     1321 ctacgattcg caaacgatgg ttgccaatac ggatttggcc gcagcagcag ccgacatcac
     1381 gatctaggct ctccattcaa aaaaagcccc ggttcagtcg tgaaccgggg ctttttagta
     1441 ttcatggctt tttcagaacg ggtgaagcct cacttaaaac gtgaaggccc gtttcttatg
     1501 ggagccggtc tgaggcgggt gaacctgctg tagatttcag cgcatcgctc aaaacattca
     1561 gattaaagga atcctgcccc aaatggatgg gcggctttaa accgctgttt ttacgagaat
     1621 atcgcccggc
//
LOCUS       DATMTI010000042         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_129140, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000042 DATMTI010000000
VERSION     DATMTI010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129140"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..843
                     /locus_tag="VN039_00720"
     CDS             64..843
                     /locus_tag="VN039_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV44543.1"
                     /translation="MTYRIKVPPRTLPVDEAHLVSGLEHWVFGLANYRWSILVGILLL
                     LLIGGGIWGVFWYDAQNAGKAQELEREATIHLFTRAANDPQKAANNLKEAIVLYKRVV
                     DEYPRTSTAPLAQFSLGNAYLQSNDLASAIEAYNRFISTYGSHVSLLGLVQQKLGYAY
                     LLKGDLDQAAKAYSAVLEIPGALNRDYALFELARLEENRSKPDEALKHYQNLMKTFPN
                     SSLTSEAAMRVKVIEAKRTPDPSPAATTPSASSSPSKPSKP"
     gene            complement(904..2205)
                     /locus_tag="VN039_00725"
     CDS             complement(904..2205)
                     /locus_tag="VN039_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWV44544.1"
                     /translation="MMTTCARQWGAQATIAAVLSAVLLLPPFESRAQGNPAGEQNNPA
                     GEIKSAQEDDGIDANLVPLEPELEPEQTISPSEPPSTDGSVELPEQTPDKTALDAPGA
                     TSADAQGPLYNIPVVIDQNVQSHIHFFNISIRNRFEQWLLRFSRYRPLVENIFAEFDL
                     PSDLVNLSLVESGFNPYAYSRAKATGPWQFMKGTGQLYGLRIDHYVDERRDPIKSTVA
                     AARYLRDLYDLFGAWPLAMAAYNAGEGKVMRALQKAQAESFSDISKTKLIRLETKQYV
                     PRIMAATIIAKNLDQYGFTQDPVPLHQFEEVVVTRPLHFRAIANVTGIPYAELRLLNP
                     ELRRDATPPNDAAYHLKVPVGTSAKVVELINRVPTYKFPPLRAESRHVKIDTARRYRV
                     RMGDTLEKVSRRFHVPLKTLKTLNHLSSPVIKAGELLVITR"
     gene            2491..>2973
                     /locus_tag="VN039_00730"
     CDS             2491..>2973
                     /locus_tag="VN039_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWV44545.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDPIFAEVREG
                     LKWLYQTRNDVLMLAASGTGGMEGAVSNFLSPGDKALYVNGGKFGERWGKLCKTFGVQ
                     ATEIKVEWGHAVNPQQVADALKKDPSIKAVYVQASETSTAVAHDVKALGEIIKGYDNT
                     I"
BASE COUNT          683 a          774 c          825 g          691 t
ORIGIN      
        1 ttagtataaa gcaaaggttc tggtgcagat accctggtct caatcatgaa gtgatgattt
       61 ctgatgacat accgaattaa agttccgcct cggacattgc cggtggatga agctcacctc
      121 gtgagtgggc ttgagcactg ggtgtttgga ctggcgaact accgctggtc gattttggtg
      181 gggatcctgc ttctccttct gataggagga ggaatctggg gcgtcttctg gtatgacgca
      241 caaaatgccg gcaaggctca ggaactggaa cgagaagcga ccatccatct ctttacgcgt
      301 gcggccaatg atccacagaa ggcagctaac aacctgaagg aagcgattgt cctgtataag
      361 agggtggtcg acgagtatcc tcgaacttca acggctccgc tcgcacagtt cagtcttggg
      421 aatgcttacc ttcagtccaa cgaccttgcg tcagcgatcg aggcctataa cagatttatc
      481 tctacctatg ggtctcatgt gtcgctcctc gggctcgtac agcaaaaact gggttatgcc
      541 tatttgctca agggggatct agatcaagca gccaaggcct actcggcggt cctcgagatc
      601 cccggcgcgc tgaaccggga ttatgccctc tttgaacttg ccagattgga agagaatcgc
      661 tctaaacccg atgaagcctt gaagcattat cagaatctga tgaagacctt cccaaattct
      721 tccctgacga gcgaagccgc catgcgtgtg aaggtgatag aagccaagag aactcccgat
      781 ccgtctcccg ctgcaacaac tcccagtgct tcttccagcc cttcgaagcc atccaagccg
      841 tagatacgct tctctgcctt ggcgagcaga acctcccact cgttcgttca aggtcggtgc
      901 gtactaacga gtgattacga ggagctcgcc agccttaatc actgggctgg acagatggtt
      961 caacgttttg agggttttga gaggaacgtg aaatcgtcgg gaaaccttct caagcgtgtc
     1021 ccccatacga acccgatatc ggcgtgcagt atcaattttg acatgccgac tttcggcgcg
     1081 caggggaggg aacttatagg tagggactcg gttgatgagt tcgactactt tggcgctggt
     1141 tcccaccggg accttcagat gatacgctgc gtcgttcgga ggtgtcgcat cccgccgcag
     1201 ctccggattc agtaatcgga gctcggcgta cggaatccct gtcacattgg caattgcgcg
     1261 gaaatgcaag gggcgggtca ccacgacttc ctcaaattgg tgaagcggga cgggatcttg
     1321 agtgaacccg tattggtcga gattcttcgc aataatggtc gcagccataa tccggggaac
     1381 gtattgcttt gtctccagcc gaatgagctt cgtttttgag atatctgaga aggactccgc
     1441 ctgagccttc tgaagggctc gcatgacctt tccttctccc gcattgtacg ccgccatggc
     1501 caagggccaa gcgccgaata aatcgtagag gtcccgcaga taccgcgccg cggcgacggt
     1561 ggatttgatc gggtcacgtc gttcgtcgac ataatgatcg atccgcaacc catagagttg
     1621 ccccgtccct ttcatgaact gccatggacc cgtggcttta gctcgtgaat aggcgtaggg
     1681 gttgaagccg ctctcaacaa gagagagatt cacaaggtcg cttggaaggt cgaattcggc
     1741 aaagatattt tcgaccagcg gacgatagcg gctgaagcgc aagagccatt gctcaaacct
     1801 gtttcgaatt gagatgttga aaaaatgaat atggctttgc acgttctggt cgatgacgac
     1861 gggaatgttg tacagcggac cttgagcatc cgcactcgtc gcgcccggag catccagagc
     1921 ggtcttatca ggtgtctgtt caggtagctc tacactgccg tccgtcgatg gaggctcaga
     1981 aggagaaatc gtctgctcag gctccagctc aggttctagg gggaccaggt ttgcgtcaat
     2041 gccatcatct tcttgggccg attttatttc accggcagga ttattctgtt caccagcagg
     2101 attaccctgg gctcgactct cgaatggcgg caacagcaga acggcactca gtacggccgc
     2161 gattgtcgct tgggcccccc actgcctcgc acacgttgtc atcatggata acctcgatga
     2221 tagcctcgat cgcatcaaag actatcgacc caccagatac ttgtcaagga actgcggtcg
     2281 gcgaagacgc tggtacattc gtgtcatgtc gggcagggag aatggtgtgc ggaaaaactt
     2341 agcttctcta gcgtcatcgc aagagaaaaa atcctgatgc agtcttcgtt tacgccggtc
     2401 gatttcttga cagcctcccg gcgcaaatga tagcgtaccg cctctcctag tggtacccca
     2461 ccagaatcgc attgtaagga ggatcgatca atgttgaagc ggtatctctt ggctcccggc
     2521 ccaacgcccg tccctccgga agtgttgctg gcgatggctc gtccgatgat ccatcatcgc
     2581 gcacccgagt tcgatccgat attcgcggag gtccgcgaag ggctgaagtg gctgtatcaa
     2641 acacggaatg atgtgctgat gttggcagcg tccggaacag gcggcatgga aggcgcggtc
     2701 tcaaactttt tgtctcccgg cgataaggcc ctctatgtca acgggggtaa atttggagag
     2761 cgctggggca aactctgcaa gaccttcggg gtacaggcaa ccgagatcaa ggttgaatgg
     2821 ggacatgcgg tgaatcctca gcaagttgca gatgctctca aaaaagatcc ttcgatcaag
     2881 gcagtgtatg tgcaagccag cgaaacctcg acggctgtcg ctcatgatgt caaggcgttg
     2941 ggtgagatca tcaagggcta tgacaatacg att
//
LOCUS       DATMTI010000043         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_723094, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000043 DATMTI010000000
VERSION     DATMTI010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723094"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VN039_00735"
     CDS             complement(<1..265)
                     /locus_tag="VN039_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44546.1"
                     /translation="MNTAITTSNHPPGVTGNRQVPERRGILYIISAPSGAGKTTLCKQ
                     VVASVSGLWHSVSFTTRKPRPGEEHGREYFFIDEKVFHDMVARN"
     gene            complement(262..1140)
                     /locus_tag="VN039_00740"
     CDS             complement(262..1140)
                     /locus_tag="VN039_00740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWV44547.1"
                     /translation="MIKSMTGFGRRQGTWSDGTVSVEVRSVNHRFLETSIRIPKWMGG
                     LEERFKKAIQQHCGRGRVDLTVLLQGGRGSARAVQLDVELAKQYHHALLTLQRTLKLK
                     GSIDIGLVAGFRDIIALSEQPTDDPKLARVVEKLGLLAVSDMTKMREKEGVLLAQDIL
                     ARLDQVRECRSAVSSRAPYMAQETFDRMKQRVEKLLADSIPDLPRLNQELALYADRCD
                     ITEELVRLDTHMVQFERVLQGTEPVGKTLDFLLQEMGREVNTIGSKANDAAIRANVVR
                     MKAELERMREQIQNVE"
     gene            complement(1137..1802)
                     /gene="nth"
                     /locus_tag="VN039_00745"
     CDS             complement(1137..1802)
                     /gene="nth"
                     /locus_tag="VN039_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWV44548.1"
                     /translation="MKKAKRMGVQSTLDRPAQIAMSLRHAMPVARVELTHRSPWELLV
                     ATILSAQCTDQRVNQVTPSLFTQYPTPQAMAKAMPTELEALIRSTGFYKSKAKNLIGC
                     AQAITAQFKGRVPDAMEELTSLPGVGRKTANVLIGAVFGKPAIVVDTHVGRVANRLAL
                     THSRDPEQIERDLQSVYPQTQWTGVSQRLLLHGRYVCLARKPRCRICPIYDVCEWEGK
                     LQK"
     gene            complement(1824..>2053)
                     /locus_tag="VN039_00750"
     CDS             complement(1824..>2053)
                     /locus_tag="VN039_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWV44549.1"
                     /translation="IPRLLVFNKCDRMPPSHVELLCRRYGAIGISALQLTTLHPLLAR
                     LEAYVRTLPTGEYQTIGLPLQDDALVLASRR"
BASE COUNT          437 a          598 c          527 g          491 t
ORIGIN      
        1 cattcctcgc aaccatatcg tgaaatactt tctcgtcgat aaagaaatat tcacgtccgt
       61 gttcttctcc tgggcgcggt tttcttgtcg taaacgacac cgaatgccac agcccggaga
      121 ccgacgccac gacctgttta cataaggtcg tttttcctgc tcctgaaggg gctgaaataa
      181 tgtacaggat tccccgacgt tcaggaactt gccgattccc cgtaacaccc ggagggtggt
      241 tacttgtggt aatcgcagtg ttcattcgac attctgtatc tgttcacgca tacgctcaag
      301 ctcggccttc attcgcacca cattggccct gatcgccgca tcgttggctt ttgatccaat
      361 ggtattgact tcccggccca tctcctgaag aagaaagtcc aacgtcttcc ctaccggttc
      421 ggtgccttgg agcacccgct caaactgtac catatgcgtg tccagcctga ccaattcttc
      481 cgtaatgtcg caccggtcgg catagagagc cagctcctga ttgagccgag gaaggtccgg
      541 gatagaatcc gccaacaact tctccactcg ctgcttcata cggtcgaagg tttcctgcgc
      601 catgtaggga gcacgggacg atactgcact cctgcattca cgcacttgat caagtcgagc
      661 caggatatcc tgtgcaagca agaccccctc tttctcccgc atcttcgtca tatccgacac
      721 cgcgagtaac cccagtttct ccaccactct tgcgagttta ggatcgtcgg tcggctgctc
      781 ggaaagtgcg atgatatcgc ggaaccccgc caccagtccg atgtcgatgg agcctttgag
      841 tttcagcgtg cgctggagcg taagaagagc atgatggtac tgcttcgcta gttcaacatc
      901 aagttgcacc gcacgagcgc ttcctcgacc gccctgtagc aacaccgtaa gatcgactct
      961 tccgcgccca caatgttgtt ggatcgcctt cttgaagcgc tcttccagcc cgcccatcca
     1021 cttcggtatg cgaatggatg tttcaaggaa acgatggttg accgatctca cttcaacact
     1081 gacggtcccg tcggaccatg tcccctgtct ccggccaaat cctgtcatgc tcttaatcat
     1141 ttctggagtt ttccttccca ctcgcaaaca tcgtaaatcg ggcaaatacg gcagcgaggc
     1201 tttcgggcga ggcacacata ccggccatga agaagcaacc gttgagacac gcccgtccac
     1261 tgggtttgcg gatacaccga ttgcagatcg cgctcgatct gctcaggatc tcttgaatga
     1321 gtcagggcca gtcggttggc cacccgtccc acatgcgtat caaccacaat ggccggtttt
     1381 ccaaacacgg ccccgatgag aacattggct gtttttcgcc cgacaccggg tagcgatgtg
     1441 agttcttcca tcgcgtctgg aactcggccc ttgaactgcg cggtaatggc ctgagcgcag
     1501 ccgatcaagt tttttgcctt gctcttataa aagccggttg atcggatcaa tgcttccaac
     1561 tcagtcggca tcgcttttgc catggcttgc ggtgtcggat attgcgtgaa taggctcggc
     1621 gtcacctgat tcacacgctg gtccgtgcac tgtgctgata gaattgtggc aaccaacagc
     1681 tcccacggag aacgatgcgt taattccact cgagccaccg gcatggcgtg gcggagactc
     1741 atggcaatct gcgcagggcg gtcgagagtg gactgtaccc ccatgcgttt ggctttcttc
     1801 atggcggccc gattgtgcag aggttatcga cgagatgcaa gcaccagcgc gtcgtcctgc
     1861 aacggcaagc cgatagtctg atattcaccg gttggcagag tcctcacgta ggcttccagt
     1921 cgagcgagga gaggatgaag ggtggtgagc tggagcgccg aaatgccgat ggctccgtaa
     1981 cgtcggcaaa gtaactcgac gtgtgatggc ggcatccggt cacatttatt aaacacgagc
     2041 aatctcggta tgg
//
LOCUS       DATMTI010000044         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_341219, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000044 DATMTI010000000
VERSION     DATMTI010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341219"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VN039_00755"
     CDS             <1..324
                     /locus_tag="VN039_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44550.1"
                     /translation="VYPWNDVTGVGETPNKAAADFEEKWKTKGLTADMYSGPAWEGGI
                     KPERPAPPKPAASPPKPGTPPKPAASPGSTAPGSPVPAAPVTNTVRTTTVTPASATQP
                     SGKSD"
     gene            complement(358..1806)
                     /locus_tag="VN039_00760"
     CDS             complement(358..1806)
                     /locus_tag="VN039_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWV44551.1"
                     /translation="MRSRLLRTKPIEQILADADQPEHRLKKTLTAWDLTALGIGAIIG
                     TGIFVLIGTAIVGDAHRPGAGPGIILSFILSGITCALAALCYAEFSAMIPVAGSAYTF
                     SYATLGEFLAWLTGWNLILEYGVACVAVAIGWSGYFSKLLKLAGVELPYWAINPPHWA
                     GGPEGSIANFPAAIIVLLITALLVIGIKESARVAGLIVLLKLGVILFFIAVGAPAVNV
                     DNWMPFMPNGFEGVRAAAAIIFFAYIGFDAVSTAAEEARNPQRDVPIGILGSLAVCTV
                     LYISVAAVITGLVPVSQIDIHAPVAEALSLVGFKWGAAIVAIGAVAGITGVLVVMMLG
                     QIRVFFAMSRDQLLGQGLSKVHPTFGTPHRATILTGVAIAIMAALFQIGEAADMTNIG
                     TFFAFVLVCFGVMLLRYTKPDLPRPFRLPLMPIVPILSITACFYLMAGLPWATWIRFV
                     VWTIIGILVYAAYGIKHSKLAAQAVNSGSLSR"
     gene            complement(1937..2074)
                     /locus_tag="VN039_00765"
     CDS             complement(1937..2074)
                     /locus_tag="VN039_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44552.1"
                     /translation="MEAIESPAHRFLLAIQWHPEFLFERHAVHRRLFEALLHAARRTR
                     G"
BASE COUNT          520 a          608 c          569 g          378 t
ORIGIN      
        1 gtctacccct ggaacgatgt cacgggcgta ggtgaaacac cgaataaggc cgctgctgat
       61 ttcgaggaaa aatggaagac aaaaggctta acggcggaca tgtattcggg tccggcctgg
      121 gaaggaggca ttaaaccgga aaggccggcg cctcccaaac cagccgcctc gccacccaaa
      181 ccgggtactc ctcccaagcc cgctgcctct cctggaagta ccgcccctgg atcgcctgtc
      241 ccggctgctc ccgtcacgaa cacggttcga actactaccg tgacacccgc ttccgcaacc
      301 cagccctcgg gaaagtcgga ctaagcaagc agcctcccgt cgatagttgc ccctgattta
      361 tcgtgacagt gaaccactgt tgacggcctg tgcggcgagt ttactgtgct tgatcccata
      421 agcggcatac acgagaatgc cgatgatcgt ccataccaca aaacggatcc atgttgccca
      481 aggcaaacca gccatcagat aaaaacatgc tgttatactc aaaataggca cgataggcat
      541 caagggaaga cgaaagggcc taggcaggtc cggctttgtg taccggagca acatgacacc
      601 aaagcagacc aacacaaaag caaagaaggt cccaatgttg gtcatatcgg ccgcctctcc
      661 gatctgaaac aacgccgcca tgatcgcgat ggccactcct gtcagaatcg tcgcccgatg
      721 tggggttcca aacgttggat gaaccttgga taacccttga cccaggagtt ggtcacgtga
      781 catggcaaag aagacgcgga tttgcccaag catcatgacg accagcacac ccgtaatgcc
      841 cgcgacggct ccgatggcca cgatggcagc accccatttg aaccccacga ggctgagcgc
      901 ttcagccacc ggtgcatgaa tgtcaatttg agatacgggg accaggccgg tgattaccgc
      961 tgccacggaa atgtagagta cggtacacac agccagcgac ccaaggatac cgatcggaac
     1021 atctcgctga gggttccggg cctcctccgc agcagttgag actgcatcga acccaatata
     1081 ggcaaaaaag atgatggccg ccgcggcccg gactccctcg aagccattgg gcatgaaagg
     1141 catccaatta tcgacgttga cagcgggagc gccaaccgcg atgaagaaga gaatcacccc
     1201 cagtttgagg agcacgatca gaccggccac gcgcgcactt tcttttattc ctatcacgag
     1261 gagggccgtg atcaacaaga cgatgatcgc agccggaaaa ttggcgatac ttccctcggg
     1321 acctcccgcc caatgcggtg gattgatcgc ccaatagggt agttccacgc ccgcgagctt
     1381 cagtaacttg ctgaaatagc cggaccaacc gatggcaact gccacgcacg ccacaccgta
     1441 ttcaagaatc agattccacc ccgtgagcca ggcaaggaac tcacccagcg tggcgtacga
     1501 aaaggtgtac gctgaccccg caaccgggat catcgctgaa aattcggcat aacacagcgc
     1561 agctaatgcg caggtgatgc cggaaagaat gaaggagagg atgattcccg gccccgctcc
     1621 cggtctgtgc gcatcgccga ctatggcggt accgattagg acgaaaatgc cggtcccaat
     1681 aatcgcgccg atcccaagcg ctgtgagatc ccacgccgtc aatgttttct tgagacgatg
     1741 ctccggttgg tcggcatcgg ccaggatttg ctcgatgggt ttcgtccgga gaagtcgact
     1801 tctcacggtc tctcttgagc ccaacgtgcc aagacttgct gtgatgggat caatgctgag
     1861 gctccatctg aggtcacagt atggcaagag acccctggag ccggttggtt gtgattgttg
     1921 gaacggtcca gggagatcat ccacgcgtcc ggcgggccgc gtgtagcagg gcttcaaaca
     1981 gccgacggtg gaccgcatgc cgttcgaaca agaattccgg gtgccattgg atagccagta
     2041 aaaatcgatg tgcgggggat tcgatcgctt cgata
//
LOCUS       DATMTI010000045         1566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_807985, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000045 DATMTI010000000
VERSION     DATMTI010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807985"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VN039_00770"
     CDS             <1..1093
                     /locus_tag="VN039_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWV44553.1"
                     /translation="VEACLNCKSCRTICPAGVDVSDLILQKRAEHPNRLAGLIFRWQA
                     RGAAFESFLRFLGNTQNMWDRPFFRKLLERITTPIMRALAPTARLPHDLVLPKLAPRH
                     LRERYAHLIPDGTDSSPTRSVAYFHGCAANYFDDGVGEAVIEVLKQHGVEPALPPQRC
                     SGTPVQTYGHIDLVRDGARFNLHSFAPYQTIVTGCASCTLMLKDYVTLFPEGNERQQA
                     EELAKKVVHISEFVAHSPQHPPMAKANGTTKRVTYHSSCHLRAAGVTKEPRQVLSSLP
                     GVNFVEMQDADRCAGGAGTYLIKDYATSQKIFDRKAHAITQTGANVVATSCPACMIQL
                     KNGVDESVQVKHVAQLLQEAYRTAEREQS"
     gene            1167..1445
                     /locus_tag="VN039_00775"
     CDS             1167..1445
                     /locus_tag="VN039_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWV44554.1"
                     /translation="MVSVQIFGQTLRAVVDESELEVPVTGPTTVKQLIEANPAQLGAL
                     LQYIKNREALITINKKIGTEDSPVRDGDVVKLSFQSRTSYDGTRDIPT"
BASE COUNT          365 a          453 c          426 g          322 t
ORIGIN      
        1 ggtggaagct tgcctgaatt gcaaatcctg ccgcaccatc tgtccggcgg gggtggacgt
       61 atccgacctg attcttcaga aacgggcaga gcatccgaac cggttagccg gtttgatttt
      121 cagatggcag gcgcgcggag cagcctttga atcgttcttg cggttcctgg ggaatacaca
      181 gaacatgtgg gaccggccct tcttccggaa gttactggaa cggatcacga caccaatcat
      241 gagggccttg gctccaacgg cacgcttgcc ccacgatctt gtgttgccga aactggcccc
      301 acggcacttg cgcgaacggt atgcgcattt gatccccgat ggaacagact cctcgccgac
      361 tcgttcagtg gcctactttc atggctgcgc cgccaactat tttgacgatg gggtagggga
      421 ggccgtgatt gaagtgttga agcaacatgg agtcgaaccg gccctgccgc cacagcgttg
      481 ttccgggact ccggtgcaaa cctatggaca catcgatctg gttcgcgatg gagcacgatt
      541 caatcttcat tcgtttgctc cctatcagac gatcgtgacc ggctgcgctt cctgcactct
      601 catgttgaag gactatgtca cgctctttcc agaagggaac gagcgccagc aagccgaaga
      661 actggccaaa aaagtggtgc atatttcaga attcgtcgcc cactcgccgc agcatcctcc
      721 aatggccaaa gccaacggaa cgacgaaacg cgtcacctac cattcatcct gccatctgcg
      781 ggcggccgga gtgacgaaag aaccacggca agtgctgtcg tcattgcccg gggtcaactt
      841 tgtggaaatg caggatgccg accgttgcgc gggaggcgca ggcacttacc tcataaaaga
      901 ctatgccaca tcacagaaga ttttcgaccg caaggcccac gccatcacac aaaccggcgc
      961 gaacgtcgtg gcgacgagtt gtccggcctg catgatccag ctcaagaacg gagtggatga
     1021 gtcggtccag gtcaaacatg tcgcccagct tctccaagaa gcttatcgga cggcagaacg
     1081 tgagcagagc tgagttcatt ctcgctgatc gtgtatagta cgggctcacg tgaagcgaaa
     1141 tagtctattc acttgttgag agttcaatgg tttcggtaca aatttttggt cagaccttac
     1201 gtgcggtcgt cgatgagagc gaactcgaag tccctgtgac aggcccaacc acggtgaaac
     1261 agctgattga agcaaaccct gcacaattgg gcgccttgct gcaatacatc aagaaccgcg
     1321 aagcgctcat caccatcaat aagaagatcg gaacggaaga ttcaccggtt cgtgacggcg
     1381 acgttgtcaa gctttcgttc cagtcgcgca cctcctacga cggcacccgg gacattccga
     1441 cgtgagccga ccagcgcatg actcccgaac agcgctacga cgccggcgcc aaactccaac
     1501 gcctcatcga tcaagttcac gacctccagg ccgggtagcc ttccgcttcg tttggcaaac
     1561 cgtgag
//
LOCUS       DATMTI010000046         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_595835, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000046 DATMTI010000000
VERSION     DATMTI010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595835"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(687..1130)
                     /locus_tag="VN039_00780"
     CDS             complement(687..1130)
                     /locus_tag="VN039_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44555.1"
                     /translation="MILSIDPGDKRVGVARWSDAGDFHFKGTLSFEEYLDYLDAVLDT
                     FDIKTIVIEDWSLRQGKGVKMTGSRFLSPQCIGAANLLSVITGAELVKQSPQILRIAA
                     MHFGIKISTRGHIDDALSAKLHGLYYLESRGILEGNGAAMLGLDT"
     gene            complement(1130..>2250)
                     /locus_tag="VN039_00785"
     CDS             complement(1130..>2250)
                     /locus_tag="VN039_00785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase A family protein"
                     /protein_id="HWV44556.1"
                     /translation="VDTDLASSMAEEGRARMDEITRELGLNPGSPKQLKELLIDRLGL
                     PILKASEKTGAPSFDKSVMPTYERMLERMEDRSATLIMEYRGWQKAVTASYEPYVKLV
                     SPDGRLHCNYNLDTTVTGRFSCSDPNLQQIPKAGDRPWNEHTKECFIPEDGYVLINAD
                     YSQLELRLGTLYAQEQKLKQVFAEGRDIFTEMATQLGMSRQDTKTLTYSMQYGAGEKR
                     IMSAFGVDREQAKQMRQNYFNTYPKFKLFSDMCSARAEQQGKVKIWTGRYRHFQYESE
                     SYKAMNSVIQGGAADVVERVMLKLFDVLDDEDECRMLLQVHDAIVFEVREELVEEYLP
                     LIREVMGNVAEACKLEDFNDVVFAVDAGPDYGSKHWSE"
BASE COUNT          523 a          627 c          581 g          519 t
ORIGIN      
        1 atggggccac cccgtaatgt ccagtgcgct agggctccgt ggaattacct ggcgcgacgg
       61 agtagctgtc ctagtagtaa ttatcatgct aggacttttg gcatgggcgc ttatctccca
      121 atcagtaact attattgctt atcaacagca agacagtgtg caaaacgcac tcatcactag
      181 gcaagacagt actattggaa aactccggga tcagatttat gatcttggga aaacgccagt
      241 agccgaagct cccaagcaaa gcgagataaa tcaagcgctt caaggcccgg aaggccctca
      301 gggttcccct ggagacaccg ggaaaacggg cgctcagggg cctaagggcg acccaggtgc
      361 tccaggtcag ccaggaccta caggcgcgcc aggaccggca gggagccccg gagcggatgg
      421 cgctgacgga gcgccggggg ctaccggccc ggcagggcct caaggagttg ctggaccgac
      481 tggggcggcg ggggcttccg tgacaagcgt gcggtgtcag tacgatgccg cacagaacac
      541 gaccgagcta gtgttcagca ccacggcggc agacggccag gtcagcgacc tcccacctgt
      601 cgtagctgcc tgcgtaccga cccaatgaaa gaaccccctc agctcgggtc tgagggggtt
      661 ctttatagct gagtggacaa cgcacgctac gtgtctaagc ctagcatggc agcgccgtta
      721 ccctcaagga ttccgcgcga ctcaaggtaa tagaggccgt ggagcttagc tgacagcgca
      781 tcgtcaatat gccctcgtgt tgaaatcttg atgccgaaat gcatggccgc aatacgaaga
      841 atctgcgggc tctgcttgac tagctccgct cccgtaatga cagacagcaa gttcgcggca
      901 cctatgcatt ggggcgacag aaaccgtgag ccggtcatct taacgccttt gccctgtcgg
      961 agtgaccaat cctctatgac aatcgttttg atatcgaaag tatccagaac tgcgtcgagg
     1021 taatctagat actcttcaaa tgatagggtg cccttgaagt gaaaatctcc tgcgtcggac
     1081 caacgagcaa ccccaacacg cttatcgccg ggatcaattg aaagaatcat tattcactcc
     1141 aatgttttga gccatagtcg ggtccggcat cgacagcgaa tactacgtcg ttgaaatcct
     1201 caagcttgca agcttccgcc acattgccca tgacctcgcg gataagtggc aggtattctt
     1261 ctacaagctc ttcacggact tcaaagacga tagcatcatg cacttgtagc agcattcggc
     1321 attcgtcttc atcgtctagt acgtcgaata gtttgagcat gacccgctca accacgtcag
     1381 cagctccgcc ctgaattacc gaattcatag ccttgtagct ctcagactcg tactggaagt
     1441 gcctatagcg gccagtccaa atctttacct taccctgttg ctctgcgcga gctgaacaca
     1501 tatcgctgaa cagcttgaac ttagggtaag tattgaagta attctggcgc atctgctttg
     1561 cctgctcgcg gtcaacaccg aaagcgctca taatgcgttt ctcacctgcc ccatattgca
     1621 tcgagtacgt gagtgtttta gtgtcctgac gactcatgcc aagctgggta gccatttcag
     1681 tgaaaatatc ccgaccttca gcaaacactt gcttgagctt ttgctcttgt gcgtagagcg
     1741 tgccaagcct caattcaagc tgtgaatagt cagcattgat gagcacgtaa ccatcttctg
     1801 ggatgaaaca ctctttagtg tgttcattcc aaggcctgtc cccagctttg ggtatctgct
     1861 ggagattcgg gtcagagcac gagaaccgac cggtcactgt cgtgtcaagg ttgtagttgc
     1921 agtgcagtct cccatccggg ctcacaagct tgacgtaagg ctcatagctt gctgtaacgg
     1981 ctttctgcca acctcggtac tccatgatga gcgtggcgct tctgtcttcc atacgctcca
     2041 gcatccgctc gtaggtaggc atcacacttt tgtcgaagct cggcgcacct gttttctcgc
     2101 tggccttgag aatcggtaag cctagcctgt cgatcaacaa ctctttgagc tgtttaggag
     2161 agccgggatt caaacccagc tcacgcgtaa tctcatccat ccgggctcgg ccctcttcag
     2221 ccatagaact tgccaggtca gtgtcaactt
//
LOCUS       DATMTI010000047         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_426145, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000047 DATMTI010000000
VERSION     DATMTI010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426145"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..521)
                     /locus_tag="VN039_00790"
     CDS             complement(42..521)
                     /locus_tag="VN039_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44557.1"
                     /translation="MNVIIVYAVALVALFVLAYVTKRRFGVLGLALAAGAMLSSLWAD
                     QLTPLVQQHGFDLTTPPLLTVVSVVLVLLPALLLLLSGPTYHKKVEQVIGALLFSLLA
                     VALLVEPLGSSLALQGDGLQVYNWFMEHRVWLVTLGLVAALVDLLFAKTPKHSKSKH"
     gene            complement(534..1445)
                     /gene="rimK"
                     /locus_tag="VN039_00795"
     CDS             complement(534..1445)
                     /gene="rimK"
                     /locus_tag="VN039_00795"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HWV44558.1"
                     /translation="MKIAILSNGPGNYSTKRLKEVAKARGHDVRVIKYRDCYASIERS
                     NPTVSYKGEDLGQFDAIIPRIASNMTKYGTAIVRQLEMQGAYTLSSSIAITRSRDKLR
                     SMQLLAKAGVGIPKTVVSRNTTDIDDLLEQLGGMPVIIKLARGTHGNGVVLAETKKAA
                     KSVLQAFYLTNDDGTNILLQEFVKESAGTDIRAFVVGSRVVASMKRQSLDDDFRSNLH
                     KGGEGTKVKLTEEERKMAVKAAKAMGLNVAGVDMMRSDRGPLILEVNASPGFGIEKVT
                     GRDVASAIIDYVEINAKRRNKKDKVGA"
     gene            complement(1442..1696)
                     /locus_tag="VN039_00800"
     CDS             complement(1442..1696)
                     /locus_tag="VN039_00800"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HWV44559.1"
                     /translation="MKVASSMGHEQIRYKIKLPVVIEGRRINASFTLADRSTQVYPVL
                     IGRATLNGKFIVDVATGNPLLEEEKLRSEALQRNIQEEQV"
     gene            complement(1721..>2185)
                     /locus_tag="VN039_00805"
     CDS             complement(1721..>2185)
                     /locus_tag="VN039_00805"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44560.1"
                     /translation="GSSHLNNTSQGGSARLVPVDEIDSQILDACIKAAEATKLQVAGV
                     DIMHDLRNDDYYLLEVNNAPQVSSGSFMEEKSVAYAEMIRSMLDVRETGKGKTVIGRA
                     EEVIFPTLGNTTLHARIDTGARSSSIWATTIQETDAGLVVRFASPDHEIYHH"
BASE COUNT          478 a          589 c          563 g          555 t
ORIGIN      
        1 gtcccgaact ttacaaagat atcattttac aggggtattt gtcaatgttt gcttttgctg
       61 tgtttcggtg ttttagcaaa aaggaggtcg acgagtgccg ccactaatcc aagcgtcacg
      121 agccacaccc gatgctccat gaaccagttg tacacctgca aaccgtcacc ttgaagtgcc
      181 aggcttgagc cgagcggttc aacgagtagc gcgacggcca ggagagaaaa taaaagcgcg
      241 ccgatgacct gctccacttt cttgtgatag gtcggtccgc ttagaagcag tagcaaggct
      301 ggaagtaaaa ccaggacgac cgacacgacc gttagcagtg gtggcgttgt cagatcaaac
      361 ccatgctgct gaacaagggg cgttaactgg tcggcccaaa ggctgctcag catagcaccg
      421 gcggcaagcg ccaagccaag cacgccaaac cggcgtttgg tcacataagc cagtacgaac
      481 aaggctacga gtgctactgc atagacgatg atgacgttca tgcttctagt atactacgca
      541 ccgactttgt cttttttatt gcgccgcttg gcgttgattt caacgtagtc gataatcgcg
      601 ctggcaacat cacgaccggt caccttctcg atgccgaagc cggggctggc attcacttca
      661 agaatcaacg gaccgcgatc cgagcgcatc atatctacac cggcgacgtt caatcccatc
      721 gctttggctg ccttaacggc cattttacgc tcttcttcgg tgagttttac cttcgttccc
      781 tcaccgccct tgtgaaggtt tgaacggaaa tcatcatcaa gactctggcg cttcatactg
      841 gcaactacgc gcgaaccgac cacgaatgcc cggatatccg taccggccga ctctttgacg
      901 aattcttgca gaagaatgtt cgtgccatca tcgttggtca ggtagaatgc ctgaagtact
      961 gatttggcag cttttttcgt ctcggcaagc acgacgccgt tgccatgcgt accacgggcc
     1021 agcttgataa tcaccggcat accgccaagt tgctccagaa ggtcgtcgat atcggtcgta
     1081 ttacgcgaga cgaccgtctt tggaatgccg acacctgctt tcgccagaag ctgcatgctg
     1141 cgcagcttgt cacgcgagcg ggtgatcgcg atggaacttg aaagcgtata cgcaccttgc
     1201 atctcaagct ggcgcacgat ggccgtgcca tacttggtca tgttcgaggc gattcgcgga
     1261 atgatggcat cgaactggcc gagatcttca cctttgtaac tgaccgtcgg attgctccgt
     1321 tcaatggagg cgtaacaatc gcggtatttg atgacacgaa catcatggcc gcgtgctttc
     1381 gccacttctt taaggcgttt cgtcgagtag tttcctggtc cattggaaag aatcgctatt
     1441 ttcatacctg ctcctcctgt atattacgtt gtaatgcttc cgaacgaagt ttctcttctt
     1501 cgaggagtgg attgcctgtg gcaacgtcga cgatgaattt tccgttcaat gtggcgcgtc
     1561 cgatcaagac cggatatacc tgagtactcc gatccgccag ggtgaatgat gcattgatgc
     1621 gtcggccttc gatgacgacc ggtagtttga tcttataccg tatttgctcg tggcccatgg
     1681 agcttgccac tttcacgcgg tcgtaatgtt caaagacctg ttagtgatga tagatttcgt
     1741 gatcaggtga agcgaaccgt acgaccagac cggcgtcggt ttcctgaatc gttgttgccc
     1801 atatcgaaga tgatcgtgct cccgtatcaa tccgggcgtg cagggttgtg ttgccgaggg
     1861 tggggaagat cacttcttcc gcccggccga tgaccgtctt gcccttgccc gtctcgcgta
     1921 catccagcat cgaacgtatc atctccgcat acgccacgga cttctcttcc atgaacgaac
     1981 ccgatgatac ttgcggcgca ttgttgactt cgagcagata gtaatcgtca ttccttaggt
     2041 catgcatgat atcaaccccg gccacttgca gttttgtggc ttctgctgct tttatgcagg
     2101 cgtccagaat ctgagaatca atctcgtcga ctggcacgag ccgtgcgctt cctccctggg
     2161 atgtgttgtt cagatgtgac gagcc
//
LOCUS       DATMTI010000048         4884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_553663, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000048 DATMTI010000000
VERSION     DATMTI010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553663"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2525)
                     /locus_tag="VN039_00810"
     CDS             complement(<1..2525)
                     /locus_tag="VN039_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV44561.1"
                     /translation="MSSRFFIERPIFASVLSIVIVVIGLAALIKLPIAQFPNISPPVV
                     QIEADYPGASAEVAADSVARTIEVQLPGIDNLLYYDSTSTNDGHVSIRLTFEIGTNVD
                     IAQVQTQNRVKLAEPQIPPEVVRQGISTLKFSPDLLAVVALSSSDPTHDTVFISNYAL
                     LRVIDNLKRIQGVGQALVFGQQNYSMRLILNPVRMAQLGLTPSDIANVVREQNRDFPA
                     GQVGREPAPKGTELTIPIMTQGRLTEVSDFENLIVRAMSDGSMIRLKDVARVELGALS
                     YVMQGRWNGKPNVFLITFLAPGANALDTVKHIRAELDDLAESFPPGLKYDIPYDTTKF
                     IEVSIKEVLKTLAEAMTLVILVVYLFLQSWRATLIPATAVPVSLIGTFAGMQALGFSI
                     NTLTLFGMVLAIGIVVDDAIVVVENVERHIREDRLSPKEAAKRAMAEVTRPVIAIVLV
                     LCAVFVPVAFLGGIIGELYKQFAITIAMAVTISGVVALTLSPALSALVLKPGGEHHET
                     GFFALFNRFFDWTRNRYSRAVDVVIKWRALSFSVFVVIVLAAFGLFRVIPPAFLPEED
                     QGYFITIVQLPDGASKQRTDEVLKKIERYFSSIPAVHSTDAMSGMNFVFNTRGPNSAT
                     MFLPLHHWDERKPNEHVQALVGAAYGEFAKIPEALILAFNAPPIRGIGATGGFSLQVQ
                     DPSGGDFRKLAAATQEFVAKAKENPAIAGIGSNFRVTAPRLFAHVNRERAKALGVPIS
                     EIFDTMQAYFGNFYINDFLKFGRVYRVQTEAEAEFRSDPADIGKVYVRSLSPSATAFS
                     GRGSAPGLGGTGPGGTMIPLDTVVDTEFTSGPDPVTHFNGFNTA"
     gene            complement(2522..3784)
                     /locus_tag="VN039_00815"
     CDS             complement(2522..3784)
                     /locus_tag="VN039_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV44562.1"
                     /translation="MSFLNHDLFRCVAVLIVSVAIAGVFGSKEEKGNAGSTPPPPAAP
                     QVEVVTVMARTVPDEPEFIGEAEASRPVEIRSQVTGILKAVLFLEGRAIKKGAKLYQI
                     DPVPFEAAYKGAQARIAEAEARLVQAKQDLARVKPLLEAQAVSQKDVDDAIAEDLAAK
                     AALQRAQADLIKAKFDFDNTRIIAPIDGLIERSRYYEGRLVSAQTDLLTVIHRIDPMY
                     VVVNVPESFLLKRRQDAIAVGLQHPGLAQLKGTVIFADGSTYNHEGVLDFADVGLRTE
                     TGARRARFVLPNPDGVLLPGQFVKVRFKGTMKDHALLVPQRAVQQGPQGPIVFVVGEG
                     DKVEIRPIKASRWQDKEWIIESGVRSGEQVVVSGFHMARPGAPVKPIPAAEPAAEPTS
                     NGKPDNSASNGGADHEVEAKPEVTKDPQ"
     gene            complement(3943..4254)
                     /locus_tag="VN039_00820"
     CDS             complement(3943..4254)
                     /locus_tag="VN039_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44563.1"
                     /translation="MLKITEQRGTEVDSLTLVLEGRLAGPWVEELQTSWHALSANGHH
                     HVVINLADVTYIDAEGKTLLARLWRQGAELRASGCLTRCVVEEIRRNEPGGPSERIVT
                     S"
     gene            complement(4438..>4884)
                     /locus_tag="VN039_00825"
     CDS             complement(4438..>4884)
                     /locus_tag="VN039_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWV44564.1"
                     /translation="GSVMFAHGAQKLLGWFGGNGFEGTMGFFTQKMGLPWLVAFLVII
                     GESLGSLGLIAGFLTRFTAAGFIAIMIGAIAAVHWPHGFFMNWFGQQQGEGFEYHLLM
                     IAMSAALVLIGGGKWAVDDVIARRLEQGERVPQQPMRKVAQGLRLF"
BASE COUNT         1044 a         1496 c         1307 g         1037 t
ORIGIN      
        1 gcggtattga aaccattgaa atgggtcacc ggatcaggac cgctcgtgaa ctcggtatca
       61 accaccgtat cgagcggtat catcgtgccc cctggaccgg tacctcccag acctggtgcg
      121 ctacctcgtc cgctgaacgc ggtggcggaa ggactcagcg accgcacgta caccttcccg
      181 atgtcggcag ggtcagaccg gaactcggcc tcggcctccg tctgcactcg atagactcgg
      241 ccgaacttga gaaagtcgtt gatatagaaa ttgccgaagt atgcttgcat cgtatcgaag
      301 atctctgaaa tcggcactcc cagtgccttc gcccgctcgc ggttgacatg ggcgaacaac
      361 ctgggcgccg tgacgcggaa attcgagccg atcccggcga tagctggatt ttccttggct
      421 tttgcgacga attcttgagt ggcggcggcc aacttccgga aatctcctcc gcttggatcc
      481 tggacttgca gcgagaaccc gcccgtcgcg ccgatcccgc gaatcggcgg ggcattgaat
      541 gcgagaatga gggcctccgg tattttggcg aattctccat aggccgcgcc gaccaaggcc
      601 tggacatgtt cgttcggctt gcgctcgtcc caatggtgca atgggaggaa catggtggcg
      661 gaattgggac cccgcgtatt gaagacgaag ttcatgccgg acattgcgtc ggtcgaatga
      721 acggctggaa tcgagctgaa ataccgttcg atcttcttga gaacctcatc ggtccgttgc
      781 ttggaggcgc cgtcgggcaa ctgcacgatc gtgatgaaat acccttggtc ctcttccggc
      841 aaaaaggcgg gaggaatcac gcgaaagagc ccgaacgccg cgagaacgat caccacaaac
      901 accgaaaagg acaaggcccg ccatttgatc acgacatcca cggctctgga atagcgattt
      961 cgtgtccaat cgaaaaatct gttgaacagc gcgaaaaatc ccgtctcgtg atgttcgccg
     1021 cccggcttca acaccagcgc cgacaacgcg gggctgagcg ttagagccac cacccctgaa
     1081 atcgtcaccg ccatggcgat cgtaatggca aactgtttat agagttcgcc gatgatcccg
     1141 ccaaggaacg cgaccggcac gaacacggca cagagaacca ggacgatcgc gatgaccggc
     1201 cgggtgactt ccgccatggc cctcttcgcg gcttccttgg gcgacagcct gtcttcgcgg
     1261 atgtgtcgct cgacgttctc gacgaccacg atggcatcgt ccaccacgat gccgatggcc
     1321 agtaccatgc cgaagagggt cagggtattg atcgagaatc ccaacgcctg catcccggcg
     1381 aacgtgccga tcaaggaaac cggaaccgct gtggcgggaa tcaaggtggc tcgccaactc
     1441 tgcaggaaca ggtagacgac gaggatgacg agggtcatcg cctcggccag cgttttgagc
     1501 acttccttga tcgacacctc gatgaacttg gtcgtatcgt aaggaatgtc atacttcagc
     1561 cccggcggga acgactcggc aagatcgtcc agttcagcac gaatgtgttt gaccgtgtcg
     1621 agcgcattgg cgccgggagc cagaaaggtg atcaggaaca cgttcggctt tccattccac
     1681 cgcccttgca tgacgtacga cagggctccc aactcgactc gcgcgacatc tttcagacgg
     1741 atcatcgacc cgtccgacat ggctcggacg atgagatttt cgaagtcgct cacttcggtc
     1801 aatcggccct gggtcataat ggggatcgtc aattccgtcc ccttgggggc cggttcgcgg
     1861 cccacttgcc cggccgggaa atcacggttc tgttcccgaa ccacattggc gatgtcgctc
     1921 ggtgtcagcc ccaactgcgc catgcgcacc gggttcaaaa tgagacgcat cgagtaattt
     1981 tgctgtccga agacgagggc ctgtccgaca ccttgaatcc gcttgaggtt gtcgatgaca
     2041 cgcaacaacg catagttcga gataaacacc gtatcatgag tcgggtcgct ggaacttagc
     2101 gcgacgaccg ccaagaggtc gggagaaaat ttcaaggtac tgatcccctg tcggacaact
     2161 tcaggtggga tctggggctc ggccagtttc acccgattct gcgtctgcac ctgcgcgata
     2221 tccacgttgg tgccgatttc gaacgtgagt ctgatgctga catggccgtc gttcgtgctg
     2281 gtcgagtcgt aatagagcag attatcgatg ccgggcagct gtacttcgat cgtccgagcc
     2341 accgaatcag ccgcgacttc cgcgctggcg ccgggatagt cggcttcgat ttggacgacg
     2401 ggggggctga tattcgggaa ttgggcaatg gggagcttga tgagcgcggc aagtccgata
     2461 acgacgataa caatggagag gaccgaggca aagatcggcc gttcgatgaa aaagcgcgaa
     2521 ctcactgtgg atccttcgta acttcgggct tagcttcgac ctcatggtcg gccccgccgt
     2581 tggacgcgct gttatccggc tttccgttac tcgtgggctc ggcagccggc tcagcagccg
     2641 gaattggttt gacgggcgct ccgggtctcg ccatgtggaa gccgctcacg accacctgct
     2701 cgccggatcg cacacccgac tcgataatcc attccttgtc ttgccagcgg ctcgccttga
     2761 tgggacgaat ttccaccttg tctccctcgc ccacgacaaa cacgatcgga ccctgcgggc
     2821 cttgctgaac cgcccgctgc ggcaccaaca gggcatggtc tttcatcgtt cccttgaagc
     2881 ggaccttcac aaattgacca ggcagcagca ctccatccgg attagggagg acgaatcgag
     2941 ctcgcctggc tcctgtttcg gtccgcaagc cgacgtcggc gaagtcgagg acgccttcgt
     3001 gattgtacgt gctgccgtcg gcgaagatga cggtgccttt cagctgtgca aggccgggat
     3061 gttgaagacc caccgcgatg gcatcttgcc gccgtttgag cagaaaactt tccggcacat
     3121 tgaccaccac gtacatcgga tcgatacgat gaatgacggt cagcaaatcg gtttgcgccg
     3181 agaccagtcg gccttcgtaa taccgactcc gctcgatgag cccgtcgatc ggagcgatga
     3241 ttctggtgtt gtcgaagtcg aacttggctt taatcaagtc ggcctgagct ctttggaggg
     3301 cggctttcgc cgccaagtcc tcggcgattg catcgtccac atctttttga ctgacggcct
     3361 gcgcttcgag cagcggcttg acacgggcga gatcttgctt ggcttggacc aagcgcgctt
     3421 ccgcctccgc gatcctcgcc tgggcgcctt tgtaagccgc ctcaaacggc actgggtcga
     3481 tctggtagag tttggcgcct tttttgatcg ctcgtccttc taaaaacagc acggctttca
     3541 aaattcccgt gacctgtgag cgaatttcaa ccggtcgaga cgcttcggcc tccccgatga
     3601 attccggttc atccggcacg gtccgcgcca tcactgtcac gacttccact tgcggagccg
     3661 cgggaggggg aggggtcgag cccgcattcc ccttctcttc tttgctacca aataccccgg
     3721 ctatcgcaac tgatacgatc agcacggcaa cacatctgaa caggtcatga ttcaaaaaac
     3781 tcatgtgatc cgccctacgt ttcaccctac tcaaattttc ccctccaagg caaacatgtc
     3841 attctaccga tgcaagtgag acggcggcaa gtccggacga acacagatgc ttatactatg
     3901 acaatgatga atcgccgacg tccgctttca tcaactcctc aatcaactcg tcacgattct
     3961 ttccgaaggt ccgcccggct cgtttcttct gatctcttcg acaacacacc tggttaaaca
     4021 gccggacgcc cgcagttccg ctccctgcct ccacagacga gccaacagcg tcttgccttc
     4081 cgcatcgatg taggtgacat ctgccagatt gatcacgaca tgatggtgtc cgttcgcaga
     4141 cagcgcatgc caactggtct ggagttcttc cacccaaggg cctgccaatc gcccttcgag
     4201 tacaagcgtc aaagaatcta cttccgtccc tcgctgttcg gtgatcttca acatattcgt
     4261 cggacgcctc ttcgccgcgc ttcaccagct tcaagccaat accgacatca caatcaccgc
     4321 cgccacattc aacaggaaaa gaacgatcag ccacatcacg ctttcctttc agttacttca
     4381 gcgcaacacc aatcgtgccg ctgtctgttc atattcattc ggctttcccg agcttcctca
     4441 gaacaaccgt aatccttgag cgaccttcct catcggctgt tgtggaactc gttcaccttg
     4501 ttcaagccgt cgagcgatga catcatccac cgcccacttc cctccgccaa tgagcaccag
     4561 ggcagcgctc atcgcgatca tgagcaggtg atattcaaac ccctctccct gttgctgacc
     4621 gaaccaattc atgaaaaatc catgcggcca gtgcacggcc gcgatcgcgc caatcatgat
     4681 ggcaatgaag cctgccgccg taaacctcgt cagaaacccg gcgatcaatc ccagactgcc
     4741 gagcgactct ccgatgatga ccagaaaagc caccagccag gggagtccca ttttctgggt
     4801 gaagaatccc atcgtccctt cgaaaccgtt tccaccaaac cagcccaata acttttgcgc
     4861 gccgtgcgcg aacatcacgc ttcc
//
LOCUS       DATMTI010000049         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_808065, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000049 DATMTI010000000
VERSION     DATMTI010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808065"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VN039_00830"
     CDS             <1..241
                     /locus_tag="VN039_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44565.1"
                     /translation="MNGLLWFSLGWIAASFFIACLGAFVFWKSDTQVKDEIDRDWKEF
                     IRKPHRGPDGRFESRKAVAKRCLERDLKTEAANNK"
     gene            238..960
                     /locus_tag="VN039_00835"
     CDS             238..960
                     /locus_tag="VN039_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HWV44566.1"
                     /translation="MSGPWTEEQITQLIDLWNSGLSAGLIGKRMGKTRNAIIGKIGRI
                     RKDKRVTRVMGEQPYIALCVLGTGNVVNKITKRAHSAVTNAVVSRVYYRNSGVLITPQ
                     WPTESVDKLIAYWKRGDSEGTIAILMDKSLPSIRAKLDHLRDYNLIERKTKPKSTGSN
                     FIDTSPAKGVTNLFEFEKAMPQKGVGILDLKFNSCRFPTGGDRSSLLYCGEPKEVGSY
                     CRCHALRAYTSPPTRQVEGRAA"
     gene            960..1487
                     /locus_tag="VN039_00840"
     CDS             960..1487
                     /locus_tag="VN039_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44567.1"
                     /translation="MGKRSSFERMPRDFYPTPYEAILPLLPHIPHARFIEPCAGDGAL
                     VRHLEKHGHTCVDAFDIEPQAEDIRTDDALTFRREDGYSHFITNPPWDREILHPLIFN
                     LCHQKPTWLLFDADWMHTKQAIPFMEMCCKIVSVGRVKWIPDSKMTGKDNCCWYKFDY
                     WSAPKHPAMIARQAA"
     gene            1484..1876
                     /locus_tag="VN039_00845"
     CDS             1484..1876
                     /locus_tag="VN039_00845"
                     /inference="COORDINATES: protein motif:HMM:NF018101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HWV44568.1"
                     /translation="MKRPDFLRASVPRPKRKYRNTKTILNGELFDSIGEMRRYVDLSR
                     LQDAGEITGLRRQVSFILCPPVRLVGEQRASAIRYVADFVYERDGKTITEDFKGFDTK
                     ESRLKRRLMKHLLGIDVVLSGPAAKRAA"
     gene            1876..2634
                     /gene="dcm"
                     /locus_tag="VN039_00850"
     CDS             1876..2634
                     /gene="dcm"
                     /locus_tag="VN039_00850"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HWV44569.1"
                     /translation="MQKLRVLDLFSGIGGFSLGLERTGGFETVAFCEIEPFPRKVLAK
                     HWPNVPCYEDVRTLTGERLAADGIAVDVICGGFPCQDLSTAGSGTGLDGDRSGLWFEF
                     ARLIREIRPQFVILENSPELLNGWMGDILRPLAQCGYDAEWDCIPARAVGAPHLRYRL
                     WLIAYPSGDGLQRQRKYPKSINPTPDAFREANPLVDAFQRKAMPFVCRGHDGVSKQVD
                     EARNYALGNAVLPQIPELIGHAILESIRSESEAA"
BASE COUNT          666 a          739 c          747 g          650 t
ORIGIN      
        1 aatgaacggc cttctctggt tctctctcgg ctggatagct gcaagctttt tcatcgcctg
       61 tttaggtgcc ttcgttttct ggaagtctga cactcaggtc aaagacgaaa tagaccgcga
      121 ctggaaagag ttcatccgca agcctcatcg tggccctgat ggccgatttg aatctcgtaa
      181 ggccgttgct aagcgctgcc tagaacgcga cctcaagacg gaggctgcga acaacaaatg
      241 agcggccctt ggacagaaga acagataacc cagcttatcg acctgtggaa cagcggctta
      301 tctgctggtc ttatcgggaa gcgtatgggg aaaacacgaa acgctattat cggtaagata
      361 ggccgaatcc gtaaagacaa gcgcgtaacc cgcgtaatgg gtgaacagcc ttatattgcg
      421 ctctgcgttc ttggcaccgg aaacgtcgtg aacaagatca cgaagcgcgc tcactcagcg
      481 gtcactaacg ccgttgtctc gcgcgtctat taccgtaact caggtgtttt gataacgcct
      541 cagtggccta cagaatccgt cgataagcta atagcttact ggaagcgcgg tgatagcgaa
      601 ggaacgatcg ctattctgat ggataaatcc cttccgtcaa tcagggcaaa gcttgatcac
      661 ctgcgcgatt ataacctgat cgagcgaaag acaaagccga aaagtaccgg ctcaaacttc
      721 atcgacacat cgccagccaa gggcgtgacc aacctattcg agtttgaaaa agctatgcct
      781 caaaaaggcg tcggcatact cgacctgaaa ttcaactcat gccgtttccc taccggaggt
      841 gaccgttcaa gtcttttgta ctgtggcgag cctaaagagg ttggctctta ttgccgctgc
      901 cacgcgctta gggcttacac gtcgcctccc acccgtcagg ttgaggggag ggcggcataa
      961 tgggtaagcg atcatctttt gagcgcatgc cgcgcgattt ttatccgacc ccatatgaag
     1021 cgattttgcc tcttctaccc catatacctc acgcgcgttt tattgagcct tgcgcaggtg
     1081 atggggcgct tgttcgccac cttgaaaagc atggccacac ttgcgtcgat gcgttcgata
     1141 ttgagcctca ggcagaagat attagaaccg acgacgcact gacctttagg cgtgaagatg
     1201 gctacagcca ttttatcacg aatccacctt gggatcgcga aattctgcat ccgctgatct
     1261 tcaatctttg tcatcaaaag ccgacttggc tgctgttcga tgccgactgg atgcacacga
     1321 aacaagccat acctttcatg gagatgtgct gcaaaatcgt ctccgtggga cgtgtaaaat
     1381 ggattcctga cagcaagatg accggcaagg ataattgctg ctggtacaag ttcgattatt
     1441 ggagcgcgcc aaagcatcct gcaatgatcg cgaggcaggc agcatgaaac gccccgactt
     1501 cctccgcgct tctgtaccta ggcctaagcg gaaataccga aacaccaaaa ccattctgaa
     1561 cggtgagttg ttcgactcta ttggtgaaat gcgccgctat gtcgatttaa gccgattaca
     1621 ggacgcagga gagatcaccg gcttgcgtcg gcaggtttcg ttcattctct gccctcctgt
     1681 gcgtcttgta ggcgagcaaa gggcgtctgc gatacgctac gtggccgact tcgtctatga
     1741 gcgagacggc aagaccataa ccgaggattt caagggcttc gacaccaagg aaagccgctt
     1801 gaagcgcagg ctcatgaagc acctgcttgg cattgacgtg gtgctgtctg gccctgccgc
     1861 gaagagggcg gcgtgatgca gaaattacgt gtcctcgatc tttttagcgg cattggaggc
     1921 ttcagccttg gccttgagcg cacgggcggc tttgagacgg tcgccttctg cgagatcgaa
     1981 cctttccccc gcaaagtttt agcaaagcat tggcccaatg tcccttgtta cgaagacgtt
     2041 agaaccctca ccggtgagcg acttgctgcc gatggaattg ccgttgatgt catctgcgga
     2101 gggttcccat gccaggatct ttccaccgcg ggatcaggca ccggacttga tggagaccgg
     2161 agcggccttt ggtttgagtt tgcaagactc attcgcgaaa ttcgaccgca gttcgtcata
     2221 ctggaaaaca gcccagagct gcttaatgga tggatgggag acattctcag gccgttggcc
     2281 caatgcgggt atgatgcaga gtgggactgc attccagcgc gcgcagttgg tgcgccgcac
     2341 ctcagataca gactttggct tattgcctac ccctctggcg acggattgca gagacagagg
     2401 aaatatccaa aatccatcaa tccaacgccg gatgcattta gggaagcaaa tccacttgtc
     2461 gatgcatttc aaaggaaggc catgcccttc gtgtgtcgag ggcatgatgg ggtttccaag
     2521 caagtggacg aggctagaaa ctatgcccta ggaaatgccg tcctacccca aatcccagaa
     2581 ctcataggcc acgctatcct tgaatctatc cgctcagaaa gtgaggcggc gtgacccttc
     2641 cgtattacaa gcgctttcct cgtgacttcc ttgaagggac aatcggcctc tcatttgagg
     2701 agaagggagc gtatgccatc gtcctcgacc tgatttacat gcgcggcggt cagcttgagg
     2761 acgacgcgcg ttacatcgct ggtcagcttg gatgcagcgt tc
//
LOCUS       DATMTI010000050         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_553728, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000050 DATMTI010000000
VERSION     DATMTI010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553728"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..715)
                     /locus_tag="VN039_00855"
     CDS             complement(83..715)
                     /locus_tag="VN039_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWV44570.1"
                     /translation="MSTLSVVQRRSLERALTTYQKYLDEALGYLEGRGIDQAAALSAG
                     LGVVRDPLPGHDHVIGRLAIPYLTPSGPVNYNFRCMKPHSCKEAGHSKYVMWSGLQVN
                     LYNVLALESAGQAIAVAEGEIDALSSTLAGIPCVGVSGATKWQDHWNNIFEDFSSIYV
                     WQEGDEAGKKFGDRVVSEVGAIRVVLPDREDVNSIWVASGAEALRARVRK"
     gene            complement(928..1329)
                     /locus_tag="VN039_00860"
     CDS             complement(928..1329)
                     /locus_tag="VN039_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44571.1"
                     /translation="MPSQSRKHRGYRSQKVFADYVRSVFPYAEPTGAGRQGRDILSTP
                     GVWFELKARAGFDPKSALKQAHLESAKEHAAFLAAHPNISQPGPHPVAVLRMNGQGEA
                     NIGEWVACMRVDTLMDLLKEAGYGPEDTRGT"
     gene            complement(1528..>2109)
                     /locus_tag="VN039_00865"
     CDS             complement(1528..>2109)
                     /locus_tag="VN039_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44572.1"
                     /translation="DEAELLIMGDPSLASGALRKFGHVKWSFHAAPTLDHMWREAEAF
                     REVHGTYPHHTVIDILMDVDYEGASEQNYWALMAELKVLARDQQTCLTIVHHTSESAK
                     GGTPPPRSAIMGKANQLPTTILTLWGDAHNETIDVAVVKNRFGPQDAMAKKFFRMKAQ
                     PSVCHIEEAEFEEVLFRDGTSVPDDEKIDLFKE"
BASE COUNT          391 a          647 c          621 g          450 t
ORIGIN      
        1 gtgccgtgca gactgacggg ctcagtggtc acctggtcgg tcccgatgaa cgtggtctcg
       61 ttcaggatga ggtacttggt cgtcacttgc gcacccttgc tctcagggcc tcggctcccg
      121 aggccaccca gatggagttt acgtcctccc tgtcgggcag gacaactcga atcgcaccca
      181 cctcggagac gactctgtct ccgaacttct tgccagcttc gtctccctcc tgccatacgt
      241 agatggagga gaagtcctcg aagatgttgt tccagtgatc ctgccacttg gtggcaccgg
      301 atacaccgac acaagggatg cccgcgaggg tggagctgag ggcgtcgatc tccccttcag
      361 ctacagctat ggcttgacct gcgctctcca gggccagcac gttgtagagg ttgacctgaa
      421 ggccagacca catgacgtac ttgctgtgcc cggcttcctt gcacgaatga ggcttcatgc
      481 atcggaagtt gtagttgacc ggacccgaag gggtcaggta cgggatggcc aggcggccaa
      541 tgacgtggtc gtgacccggc agggggtcac gaactacgcc aagtcctgcg gagagcgcgg
      601 ctgcctgatc gattcccctt ccttccaggt accccagagc ctcgtccaga tacttctggt
      661 acgtggttag ggccctttcc agagacctcc tttgcacgac ggacagcgtc gacatagggg
      721 atgttctcct tcttcatgat gagctgtgtg gcgttgccct tcatgtcaca tgtgtgacag
      781 acgaacacat gaaggatggt gttgaccgag gctgatgctg ttgagtcccc gtggaacggg
      841 cacttgtagg cgtaccagcc tcgatcctcc atcacgtcat cgccaccgta ctctatgagg
      901 atgggaccga tcgggaagac cggccattca cgttcctcgt gtgtcctccg gaccatagcc
      961 cgcctcctta aggaggtcca tcagggtgtc gacccgcatg caggccaccc actcaccgat
     1021 gttggcctcg ccctgcccat tcatcctgag tacagcaacg ggatgcggac caggctgcga
     1081 gatgttcggg tgtgcagcca gaaaggctgc atgctccttg gctgactcca gatgcgcctg
     1141 cttgagggcc gacttgggat cgaacccggc ccgcgccttg agttcgaacc agacgccagg
     1201 cgtactgaga atgtcacgac cctgacggcc tgcccctgta ggttcagcgt aagggaatac
     1261 cgaccgaacg tagtcagcga agaccttctg cgaccggtat ccacggtgct tcctactctg
     1321 cgatggcact agtggtccag gcagggatag ggcctgccac accagcatcg gccgttgaac
     1381 ttcgggcacc agcacttgga cttgggccag ttgcaaaccg ggcagcgggc catcacgact
     1441 ccctgaactg ctggcagttg ctgcacttga gcccgtactt gccgttctcc cacgtgggga
     1501 tcaggcacgg cttaccgcaa cacatggcta ctccttgaac agatcgatct tctcgtcgtc
     1561 aggaactgac gttccgtccc tgaatagcac ctcttcgaac tcggcctctt cgatgtggca
     1621 cacagacggc tgcgccttca tgcggaagaa cttcttggcc attgcgtcct ggggtccgaa
     1681 cctgttcttc accacggcca catcgatggt ctcgttgtga gcgtcacccc agagggtgag
     1741 gatcgtagtg gggagctggt tcgccttgcc catgatggcg ctacgaggcg gaggagtgcc
     1801 gcccttggcg gactccgacg tgtggtgaac gatggtaagg catgtctgct gatccctggc
     1861 caggaccttc agctctgcca tgagggccca gtagttctgc tcactggctc cctcgtagtc
     1921 gacatccatc aggatgtcga tgacggtgtg gtgcgggtac gtaccgtgca cctcacggaa
     1981 tgcctcggcc tccctccaca tgtggtcgag ggtgggcgct gcatggaacg accacttgac
     2041 gtggccgaac ttccgcagtg caccgctagc caacgacgga tcgcccatga tgagcaactc
     2101 tgcctcatc
//
LOCUS       DATMTI010000051         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_44418, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000051 DATMTI010000000
VERSION     DATMTI010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44418"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VN039_00870"
     CDS             complement(<1..1264)
                     /locus_tag="VN039_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44573.1"
                     /translation="MSRTILVLFTVYLCSGCVSNGGIDQGRLKLKDPRSADPFDVVDC
                     LLPGQIRQLGMQVTYVTERRPVRNTAEDCAIRGGEYVALDRADYGTALKVWMSAAQGG
                     DPDAQYYVGTLYEKGAEGQPNYAKAADWYRQAADRGIRRAAINLGRLYEQGLGVEKNG
                     SEARKWFAKANGAVEPTGGGLSQAETAQQELADAHARLAERMSLFEGEQRKLEQLKQR
                     LGSSTQDASVADAVSKQERIARLRQEEVAKLQTQVASLKEATQRRLAEAQQAPVQDQR
                     LRGPSIQIIDPSLVMTRGIRVEDGRVALVIPRGRPHQVTGRVIAEGGVRTLEVNGMQT
                     QFDEQGMFVVPLESLKQATKGIPVDIVAVDRQGKLESKKFLVSSGETPLLIHRAISTI
                     DRVGGYHALVIGNDHYRQWPPLDTAVSDA"
BASE COUNT          307 a          473 c          383 g          383 t
ORIGIN      
        1 ccgcatcaga cacggcggta tccaagggtg gccactgacg ataatgatcg ttcccgatca
       61 ccaatgcatg gtatccgccg acacgatcga tcgtggaaat cgctcgatgg atcaggagcg
      121 gtgtctctcc tgaactgacg aggaattttt tgctttctag ttttccttgt cgatcaacgg
      181 ccacaatatc gactggtatg cctttcgtgg cttgcttcaa ggattctaga ggcaccacga
      241 acatcccctg ttcatcgaac tgggtctgca tcccattgac ctccagggtc cgaactcctc
      301 cttccgcgat aacccgcccc gtcacttggt gcggccgtcc tcgtggaatc accagagcta
      361 ctcgtccgtc ctctacacgg attccgcgtg tcatcaccaa tgagggatcg ataatctgaa
      421 tagatgggcc tcgcaatcgc tggtcctgca caggagcctg ctgtgcctcc gccagtcgtc
      481 gctgagtcgc ttcctttaag gaagcgactt gtgtctgcag tttggcgact tcctcttgac
      541 gcaatcgggc tatacgctcc tgcttggata ccgcatccgc cacagacgca tcttgtgtcg
      601 acgagccgag tcgctgtttc aactgctcaa gttttcgctg ctccccctcg aataaactca
      661 ttcgctcggc cagtctggcg tgggcatcag ctaattcttg ctgtgcagtt tccgcttggc
      721 tcaaacctcc gcccgtcggc tctacggcac cgtttgcctt cgcaaaccat ttacgcgctt
      781 cactcccatt cttctcaacg cccaagcctt gctcgtaaag gcgccccagg ttgatggctg
      841 cgcgcctgat tcctcgatca gcggcctgac gataccagtc ggcggccttt gcataattag
      901 gttgtccctc cgcccccttt tcgtagagcg tcccgacata gtattgcgca tcgggatcgc
      961 ctccttgagc ggcactcatc cataccttca acgccgtccc ataatccgca cgatccaatg
     1021 ccacatactc tccgccccgg atggcacaat cctctgccgt gttcctcacc gggcgtcttt
     1081 ccgtgacata cgtcacctgc attcccagct gacgaatctg gccgggtagg agacagtcca
     1141 ccacatcgaa gggatctgca gatcggggat ctttcaactt cagtcgcccc tggtcgatcc
     1201 ctccattgga aacacatccg gaacataggt agacggtgaa gagcacgagg atggttcgac
     1261 tcaagcaagg gagggcggtt tttatacggt acgtactcat ttccggcctg tgcgctgaac
     1321 gtagtttact ctaaaccaat tcatcttcca atgcgctcat gacagcgttc actgaaatag
     1381 cgatggcgtg gataatggtc gaacgccttc aggagaaagg ctgaaaacgc gattgttaga
     1441 ccgattgagg gatctgatct gccggctcca agggaaggtc caccggtgta gattcaggcg
     1501 gcatctcgcc cgcgctttct tggtcggaga gttttgccga tttctt
//
LOCUS       DATMTI010000052         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_256479, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000052 DATMTI010000000
VERSION     DATMTI010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256479"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3157)
                     /locus_tag="VN039_00875"
     CDS             complement(<1..3157)
                     /locus_tag="VN039_00875"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HWV44574.1"
                     /translation="MNISGGKTGSHTIGVSAGCNATFDAVAWFKRSDPFTSNIPPGWQ
                     SFAGHIDDCSSAIEAPPAAGSYFFMQAYDLSGQLVVNVVSPNPKPQSCVWQDASKGGH
                     ISEWDQAAHPVGNANAPIWAPNPPAQQAVSGEKKNDGDPVELATGLFIMEQIDLAIPD
                     LIPLTLTRTYRQNDTALRPFGIGATHPYDIWLTWDDYCSVMQLILPDGARETYTRTSG
                     TDCFTASLVNQSSPSAALQSTLNWDGSRQRWSLRRKDGTIYRFSSMTAYAQLLLMEIE
                     DRNGNRMSLVRDSLGRLLNVLSPSGKFLQFTYDASNRITQVSDNAGRTVTYVYDGSGR
                     LWKVTNPASGITEYTYDTSHRMLTIKDPKNITYLTNTYDANGRIATQTQADNTTFQFA
                     YTLDGSGKVTQTDVTDPRNIVRRATFNSDGYALTDTAAFGEPEAQTLTYERQAGTNLI
                     LSVTDALNRKTAYAYDAKGNLLTVTRLATTPNAVTTTFTYEPTYSQVATITDPLNHTT
                     TLGYDSTGNLTTFTNALSEITTLTVNPQGQPLTITDPLNNTTTLTYTQGDLTTTTDPL
                     TRVSTRFVDAAGRLLALTDPNKSRTQYDVDALNRVTKLIDPLNGQTQMAYDPNSNLLT
                     VSNAKSQVTTYTYNTMDRLATRQDALLNTETYTYDNNGNIATVTDRKAQVTTYTYDVL
                     NRRTKATFQDGTSTNYTYDAGNRITQVQEKDSGGTVTTTITRTYDGLDRLTQEVTPQG
                     TVNYTYDNASRRATMTVVGQPQVTYTYDNADRLTQVQQGTSTTTIAYDIAGRRASLTL
                     PNTNSIVYAYNAASELTSLTYKQGATTLGDLTYTYEPAGNRIKTGGTFARTNLPPALA
                     TANYNANNQQTTFGGSTETYDLNGNLATVMDAGGTTTYTWNARNQLTSISGPSLTASF
                     TYDSFGRRTGKTINGTTTNFLYDGLNPVQEKNGGTVTANLLTGLGIDEFFTRTDGGGV
                     RSLLPDTLGSTVALGDNTGTLQTQYTYEPFGVTTQTGSASTNSYKYTAREDDGTGLYY
                     YRARYYQPQTQRFIAEDPLGFG"
     gene            complement(3099..>3323)
                     /locus_tag="VN039_00880"
     CDS             complement(3099..>3323)
                     /locus_tag="VN039_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44575.1"
                     /translation="RAHEHSTTSLRCNGGTVWWDLCPNQSAHQLVEQWRDCRWKCDRR
                     RDDLAKLRIRDREHFWWEDWEPYHRGVGGM"
BASE COUNT          633 a          876 c          979 g          835 t
ORIGIN      
        1 ccccaaaccc caacggatct tccgcaataa accgctgcgt ttgtggctga taatatcgtg
       61 cccgatagta gtacagcccc gtgccatcat cctccctggc ggtgtacttg taactgttgg
      121 tgctggctga tcccgtctgt gtggtcacgc cgaacggctc ataggtgtac tgagtctgca
      181 gcgtgccagt gttgtcgccg agggccacgg ttgagccaag ggtatcgggt aacaacgagc
      241 gaacgccgcc tccatcagtt ctcgtgaaga attcgtcgat gccaaggcca gtcaatagat
      301 ttgccgtgac cgtcccgccg tttttttcct gcaccggatt gagtccgtca tagagaaaat
      361 ttgtggtggt gccgttgatc gtcttgcccg tgcggcggcc aaaggaatcg taggtgaagg
      421 acgcagttaa actcgggccg gagatgctgg tcagttggtt tctcgcattc caggtgtacg
      481 tggtcgttcc acccgcatcc atcaccgtcg caaggttgcc gttcaggtca tacgtctcgg
      541 tgctcccgcc gaacgtggtt tgctgattat tggcgttgta gtttgccgtg gcgagtgccg
      601 gaggaagatt ggttcgggca aaagtgccac cagtctttat ccgattgccc gccggttcgt
      661 aggtgtaggt caagtcgccg agggtcgtgg caccttgctt atacgtcaag ctcgtaagct
      721 ctgaggccgc gttgtacgca tagacaatgc tattcgtgtt aggcagggtg agactggctc
      781 ttcgtccagc gatatcatag gcgatggtgg tggtactggt tccctgttgg acttgcgtaa
      841 ggcggtcagc attgtcatag gtgtaagtca cttgcggctg cccgacgacc gtcatggtag
      901 cgcgacgact ggcattgtcg tacgtgtagt tcacggttcc ctgcggcgtg acttcttgcg
      961 tgagccgatc caggccgtca taggttcgcg tgatcgtcgt cgtgacggtc cctcctgagt
     1021 ccttttcctg cacctgtgta atgcgattcc cagcatcata cgtgtaattt gtactggtgc
     1081 cgtcctgaaa cgtcgctttc gttcgtcgat tcagcacatc gtacgtatag gtcgtgacct
     1141 gtgctttgcg atcggtcacc gtggcgatgt tgccgttgtt gtcatacgtg taggtctccg
     1201 tattgagcaa cgcatcttgt cgcgtcgcca gacgatccat ggtgttgtac gtataggtcg
     1261 tcacctgact cttggcattt gacaccgtca gcagattgct attgggatcg taggccattt
     1321 gtgtttggcc attgagggga tcgatcagct tcgtgacgcg attcaacgca tccacatcat
     1381 attgagtgcg gcttttattc ggatcggtga gcgccagcag ccggcccgct gcatcgacga
     1441 accgcgtgga cacgcgagtc aacggatcag tcgtggtggt gagatctccc tgcgtatagg
     1501 tcagggtggt ggtattgttc aacggatcgg tgatcgtgag tggctggcct tgagggttca
     1561 cggtcagagt ggtgatttca ctgagcgcat tcgtaaacgt cgtcagattg cctgttgaat
     1621 cataccccaa cgtggtcgta tgattgagcg gatcggtgat cgtggccacc tgactgtacg
     1681 tcggctcata ggtgaatgtc gtcgtcacgg cattcggcgt ggtcgctaac cgggtcacgg
     1741 tgaggagatt acccttcgca tcgtaggcgt atgctgtttt gcgattaagc gcatcggtca
     1801 cactcaggat cagattcgtc cctgcttgcc gctcataggt gagcgtttgg gcttcgggct
     1861 cgccgaaggc cgccgtatcg gtgagcgcat atccgtcgct gttgaaggtg gcccgtcgca
     1921 cgatgttccg tggatccgtg acgtcggttt gtgtcacctt gccgctccca tccagcgtat
     1981 aggcaaactg aaaggtcgtg ttatcagctt gcgtctgcgt ggcgatccgt ccattcgcat
     2041 cgtaggtgtt cgtcaaatac gtaatgttct tcggatcctt gatggtcagc atgcgatggc
     2101 tggtgtcgta ggtatattcg gtgattccac tggccggatt agtgactttc cacaagcgac
     2161 cactgccatc atagacatag gtcacggtgc gtccggcatt atcgctcacc tgcgtgatgc
     2221 ggttgctcgc atcataggtg aactggagaa acttgcccga tgggctgagc acattcagca
     2281 accggccgag cgaatcgcgg accagcgaca tgcgattacc gtttctatct tcgatctcca
     2341 tgagcaagag ttgggcatag gccgtcattg atgagaaacg atagatcgtg ccatccttgc
     2401 ggcgcagact ccaccgttgc cggctaccat cccagttgag cgtggattgc agcgcggcgc
     2461 ttggggagga ctggttgacc aaggaggccg tgaaacaatc ggtgccgctc gtgcgcgtgt
     2521 acgtctcccg cgcgccgtcc ggaagaatca attgcatgac gctacaatag tcatcccagg
     2581 tcaaccagat gtcataggga tgggtagccc caattccaaa gggacgtaaa gctgtgtcat
     2641 tttggcgata ggtccgtgtg agcgtcagtg ggatcagatc ggggatggct aagtcgatct
     2701 gctccatgat aaagagtccc gttgctagtt cgactggatc gccatcgttt ttcttctcgc
     2761 cactaacggc ctgttgtgct gggggattag gtgcccatat tggggcattg gcattgccga
     2821 caggatgggc ggcttgatcc cactcactga tgtgtcctcc cttggaggca tcctgccaca
     2881 cacacgattg cggttttgga ttcggagaca ccacgttgac cacgagctgg ccgctcaaat
     2941 catatgcctg catgaaaaag tatgaaccgg cggccggcgg ggcttctatc gcggacgagc
     3001 agtcgtcgat atgccctgca aagctctgcc atccgggagg aatgttcgat gtaaatgggt
     3061 cggaccgttt gaaccacgca acggcatcga aggtggcatt acatcccgcc gacaccccga
     3121 tggtatggct cccagtcttc ccaccagaaa tgttcacggt ccctgatccg cagtttggca
     3181 agatcatccc gcctccgatc gcacttccag cgacagtcgc gccattgctc gaccagctga
     3241 tgggcggact gattaggaca tagatcccac cacacagtac caccattgca acggagagat
     3301 gtcgtcgagt gttcatgggc tcg
//
LOCUS       DATMTI010000053         1655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_2195, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000053 DATMTI010000000
VERSION     DATMTI010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VN039_00885"
     CDS             <1..1491
                     /locus_tag="VN039_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44576.1"
                     /translation="ERTREREVHVSFVLEKVGLSVFRNKNITSILQRDLPASEYIIDF
                     PAAEAGATDQTMLTHVTQYMSDLLRPGEWVRGVKTISSVIQRKYVVIDLDDIAKGVIP
                     EGSSETLLCGLKETIKTGVLPKLAEEEEHALPSITTSFSADQLIVGSEEQLAQFERIV
                     GDAQTDVFVLSTFVAPQNDEKCDAKGKAQRERIWQALEKACLRGVRCHLFFGTSINPI
                     DNTRNIQELSDRLSALRKTAGYVLVHRDSVKSHAKFVAADDGQGGVSVLMGSCNWLSS
                     PFAALEVSVELRESNAVAMGLDLLTSIVSSLSSASRSVETLQFMAAELRRKKHRLESP
                     LDASPANQAQLTVVFADDHERLLREVAHDADKRFICCTNKVGANMVPALFTPAEVAGR
                     RLDDVRIYYSRQSGPVKRRHVASHRERLNGIVDLIGVRTPQVHGKFLAWDEDHIVVSS
                     LNWGSQSGSAKNRLDEIGLYLRREGLACELLKKFEAALSDRKQEIH"
BASE COUNT          394 a          427 c          458 g          376 t
ORIGIN      
        1 gagcgcacga gggaacgtga ggttcatgtt agtttcgtct tagaaaaggt cggtttgtcg
       61 gtcttccgaa ataaaaacat tacgtcgatc ttgcagcggg atttgcctgc cagcgaatat
      121 attatcgact ttccagcggc cgaggctgga gcaaccgacc agacgatgct gacacacgtc
      181 acccaataca tgtctgactt gctacggccc ggtgaatggg tgcgcggtgt gaagacgata
      241 agctccgtta tccaacgtaa atacgtggtc attgacctcg atgacattgc aaaaggcgta
      301 attccagagg gcagctccga aaccctgcta tgcgggctta aagagacaat taaaactggc
      361 gtgcttccta aactcgcaga agaggaggag cacgccttac cttcgatcac gacttccttt
      421 agcgccgacc aattgattgt cggctctgag gagcaacttg cgcagttcga gcgtatcgtg
      481 ggcgatgccc aaacggatgt cttcgtcctt tcgacatttg ttgccccaca aaacgacgaa
      541 aaatgcgatg caaaaggcaa ggcgcagagg gaaagaatct ggcaagcgct tgaaaaggct
      601 tgtctccgag gtgttcggtg tcaccttttt ttcggcacat ctataaatcc gatcgacaac
      661 acgagaaata ttcaggaact cagtgatcgc ctgtcggcac tccgcaagac ggcaggctat
      721 gttctcgtgc atagggatag cgtcaagagt cacgcgaaat tcgttgcagc cgacgacggt
      781 caaggcggcg tgtcggttct catggggtcc tgcaattggc tttctagccc ttttgcggcc
      841 ctagaagttt ccgttgagct gcgagagagc aacgccgtcg caatgggact ggacttactg
      901 acatcaattg tctcgtcact ttcgagtgcg agccgctcag tggaaacgct ccaattcatg
      961 gcagctgaac tgcggcgcaa aaagcaccga ctggaatcgc cattggatgc ttcaccggcc
     1021 aatcaggctc agttgacggt tgtttttgct gacgatcacg agcggttatt gcgcgaagtt
     1081 gcccatgacg ccgacaaaag gttcatttgc tgtaccaata aggtgggcgc caatatggtg
     1141 cctgccctgt tcactccagc tgaagttgcc ggccgtagat tggatgatgt tcgcatttat
     1201 tattctcgac aatccggccc cgtaaagcgc aggcacgtag cgagccatag ggaacgttta
     1261 aatggcatag tcgatctcat tggtgttcgc acacctcagg tgcacgggaa atttctcgcg
     1321 tgggacgagg atcatattgt ggtgagcagc cttaactggg gctcccaatc agggtcagct
     1381 aagaatcggc tcgatgaaat tggactttac ctgcgccgtg agggcctcgc ttgcgaatta
     1441 cttaagaaat tcgaagccgc gctcagcgat cggaagcagg aaattcactg actttctggc
     1501 aacctggatg tccagccagg ccggttcggc gacgtctcgc tcacccgtca ttggggccgc
     1561 atcggatcga atggccatcg aagcaacatt ggttctcaga cacggacgcc gccgacgccc
     1621 tggtcggcag cctgcgacgg cagaagaaac gcctc
//
LOCUS       DATMTI010000054         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_129437, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000054 DATMTI010000000
VERSION     DATMTI010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129437"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VN039_00890"
     CDS             <1..1265
                     /locus_tag="VN039_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWV44577.1"
                     /translation="PGPVKGFLDSAQIVDLAKRIGADAVHPGYGFLSENAEFAELCQA
                     SGITFIGPSTRAITLMGSKVKAREIAESAGVPIVPGTDGAITGIKDALAFVKKSGYPV
                     MIKASAGGGGRGLRVVRSDDELRENMEVAAREAQASFGDGSVFIEKYIERPHHIEFQI
                     LGDRHGNIIHLNERDCSIQRRHQKLIEIAPSLILTQALREEMGNAAIAIARAVNYDNA
                     GTVEFLLDQEGKFYFIEMNPRLQVEHTVTEQITAIDIVRNQIKIAAGLPLNIQQKDVI
                     LQGHAIQCRINAEDPKNNFLPCTGTVTAYLSPGGIGVRIDGAVYKDYTIPPYYDALLA
                     KLTVRGRTWEEAVSRMRRSLEEYVLRGVKTTIPFMEAIMQERDFIAGRFDTSYLDTHP
                     ELYSYHEFEQPEDLVLALSAAIAAYEGL"
     gene            1310..>3134
                     /gene="oadA"
                     /locus_tag="VN039_00895"
     CDS             1310..>3134
                     /gene="oadA"
                     /locus_tag="VN039_00895"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HWV44578.1"
                     /translation="MAKRRPKRPQKKTRSSAPTIKTLKTVAARPSEFTITPAAGKPVL
                     ITDVALRDGHQSLLATRMRTEDMLPIAQKLDTVGYWSLEVWGGATFDTCLRFLKEDPW
                     ERLRALRAAMPNTKLQMLLRGQNLVGYRHYADDVVERFIERSATNGIDVFRIFDALND
                     VRNLDRAVSEVKACGKHAEATICYTVSPVHSIDRFVDLARKLEDLGTDTICIKDMAGL
                     LAPLDAYHLVRRLKAAVKVPLHLHSHYTSGMASMASLMAILGGLDMLDTSISPLAGGT
                     SHPATETLVASLQNTPYDTGLELASFQPITEHFRMVRRKYRQFESDFTGVDAEILMSQ
                     IPGGMLSNLAAQLTEQNALDRMKEVLDEVPRVRKEMGYPPLVTPTSQIVGTQATLNVL
                     TGERYKVITTETKNYFLGLYGRAPGQVDLDVMARATGDDTPIKTRPADRLEPELDEAK
                     KELPDSAQTIEDQLSFVLFPSIARDFFEAREKGELTPEPLELGSAKGPATAHELHLAP
                     VEFNVTVHGETYHVKVSGSGRKIDGRKPYYIRVNDKLEEVSIEPIQEVLAGVPESQEP
                     QSGGKPKRPRPSKPGDVAPPMPGRVVKILVAVDDRVKVGDPL"
BASE COUNT          719 a          919 c          881 g          615 t
ORIGIN      
        1 ggcccggccc ggtgaagggt ttcttggata gcgcacagat cgtagacttg gcgaaacgga
       61 tcggcgccga tgccgtccat cccgggtacg gatttctttc tgaaaatgcg gagttcgccg
      121 agctctgcca ggcatccggc attacgttca ttggtccttc tacccgggcc attaccctga
      181 tgggcagcaa ggtcaaagcg cgcgagatcg ccgaatctgc cggtgttccg atcgtgccgg
      241 gcacggatgg agccattacc ggtataaaag acgccctggc cttcgtaaaa aaatccggct
      301 acccggtcat gatcaaggcg agcgccggcg gcggcggccg cgggcttcgc gtggtccgat
      361 cagacgatga gctgcgagag aacatggaag tcgccgcgcg agaagcccag gcgtccttcg
      421 gcgacggcag cgtcttcatc gaaaaatata tcgagcggcc gcaccacatt gaattccaaa
      481 tactgggaga ccgtcacggg aatatcatcc atttgaatga acgtgattgt tcgatccaga
      541 gacgccacca gaagttgatt gagatcgcgc cgtccttgat tctgacgcag gcgctccggg
      601 aagagatggg caacgccgcc atcgccatcg cacgcgcggt gaactacgac aatgcgggaa
      661 ccgtggagtt tctgctcgat caagaaggca aattctactt catcgagatg aatccgcgcc
      721 tccaagtcga acacacggtg acggaacaaa tcacggccat cgatatcgtg cgaaatcaga
      781 ttaagatcgc ggccggcctg ccgctcaaca ttcagcaaaa ggacgtcatc ctccaaggtc
      841 acgccatcca atgtcggatc aatgcagagg acccgaaaaa caatttcctg ccatgcacgg
      901 gcactgtcac ggcctatctc tctcccggtg gaatcggtgt tcgcatcgac ggtgccgtct
      961 ataaggatta cacgatcccg ccgtactatg atgcgctgct ggccaaattg acggtccggg
     1021 gccgcacctg ggaagaagcc gtcagccgta tgcgccgctc tctcgaagag tacgtgctcc
     1081 gtggagtgaa aaccacgatt cccttcatgg aggcgattat gcaggaacgg gactttattg
     1141 cgggacgctt cgacacatcg tacctggaca cacaccctga gttgtactcg taccacgaat
     1201 ttgagcagcc ggaggatctg gtgttggcgc tgtctgccgc cattgccgcc tacgaaggtc
     1261 tctaattcga acaaaccatt ctgcttccca aagagatgcg ctgaacatca tggcgaaaag
     1321 acgaccaaag agaccgcaga aaaaaaccag atcgtccgct ccgacgatca agactttgaa
     1381 aaccgtcgcg gcccgcccca gcgagttcac gatcacgccg gctgccggca aaccggtcct
     1441 gatcacggat gtcgcgctcc gagacggcca tcaatcgtta ttggcgacgc gcatgcgcac
     1501 ggaagacatg ctgcccatcg cgcaaaagct cgacacggtc ggctattggt cgctggaagt
     1561 atggggcggc gccaccttcg acacctgttt gcgcttctta aaagaagatc cctgggaacg
     1621 gctccgggcc ttgcgggccg ccatgcccaa caccaagctc caaatgttgt tgcgcgggca
     1681 aaacctcgtc ggctatcgcc attacgccga cgacgtggtg gaacggttca tcgaacgttc
     1741 ggccacgaac ggcattgatg tcttccgcat cttcgacgcc ctcaacgatg tgcggaatct
     1801 cgatcgggcg gtgagcgaag tgaaagcttg cggcaaacat gctgaagcca ccatctgtta
     1861 caccgtcagc ccggtccata gcatcgatcg ttttgtggac ttagccagga agttggaaga
     1921 tctgggaaca gatacgattt gtatcaagga catggccgga ctcttggcgc cgctcgacgc
     1981 ataccatttg gtgcgtcggc tgaaggccgc cgtcaaggtc ccgcttcatc tccactcaca
     2041 ctatacgtcc ggtatggctt ccatggcttc gttgatggcg atcctcggag gccttgacat
     2101 gctcgatacc tcgatctctc cgctggcagg aggcacttcc catccggcca cggaaacgtt
     2161 ggtcgcgtcg ctccagaata cgccctacga tacgggattg gagctcgcct ccttccaacc
     2221 gatcaccgag cacttccgaa tggttcgccg caaatatcgc caattcgaga gcgactttac
     2281 cggcgtggat gcggaaatcc tcatgtcgca gattcccggc ggcatgttat cgaacctcgc
     2341 cgcccaactg acggagcaaa atgcgttgga ccggatgaag gaggttctcg acgaagtgcc
     2401 gcgtgtgcgg aaagaaatgg gttatccccc gctcgtcacc ccgaccagcc aaatcgtcgg
     2461 aacacaggca accctcaatg tgctgaccgg cgagcgatac aaagtcatca ccaccgaaac
     2521 caagaactat ttcttgggat tgtacggccg tgctcccgga caggtcgacc tcgacgtgat
     2581 ggcacgcgcg accggtgatg acacgccgat caaaaccagg ccggctgatc gacttgagcc
     2641 ggaattggat gaggcgaaaa aagagcttcc cgattcggct cagaccatcg aggaccagct
     2701 gtcgttcgtg ttgtttccca gcatcgcacg agactttttc gaagcccgtg agaaaggcga
     2761 gttgacccct gagccgctcg aattgggctc tgcgaaaggg cctgccaccg ctcacgaact
     2821 gcacctggct cccgtggaat tcaatgtgac agtgcacggc gaaacctatc atgtaaaggt
     2881 ctctggctct ggtcgaaaga tcgacggacg taagccctac tatatccgtg tgaatgataa
     2941 gctggaagaa gtgtcgatcg aacccatcca ggaagtgctc gccggtgtcc cggagtctca
     3001 ggagcctcaa tcgggaggaa aacccaaacg tcccagacct tcgaaaccgg gcgatgtggc
     3061 tccacccatg ccgggtcgag tggtgaagat cctggttgcg gtcgacgatc gggtcaaggt
     3121 cggcgatcct ctgt
//
LOCUS       DATMTI010000055         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_383853, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000055 DATMTI010000000
VERSION     DATMTI010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383853"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VN039_00900"
     CDS             <1..1225
                     /locus_tag="VN039_00900"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWV44579.1"
                     /translation="VGFFHCNILLALGGVIGVFLAIDLFLFFFFWEVMLVPMYLLISV
                     WGHANRRYASFKFFLFTQAGSLLLLVAIITLAFLHQQATGKPSFDYADLMGLTLNKGM
                     AWWLMLAFFIGFVVKLPVPPFHTWLPDTYTEAPTGASIILAGLLAKTGAYGLLRFTVP
                     MFPEVISDFAPIAMGLGAMGILYGAVLACAQTDIKRLVAYSSLSHMGFILLGAFSGTE
                     LALQGAVMQLVAHGLSTGALFMLVGALYERFETRDMRQMGGLWGVIPRGASMALFFAC
                     ASLGLPGLGNFIGEFLVLFGSYSAQPMVAILASIGMVMAAIYSLGMMQRTFFGRQRET
                     RLSPDLSNVAFGTLLLIAVLQVWFGLYPRPVLSTTRPVMETLVQPALPLPNLTHSPSE
                     LMQSSNLFTPNVSTP"
     gene            1222..>2654
                     /locus_tag="VN039_00905"
     CDS             1222..>2654
                     /locus_tag="VN039_00905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWV44580.1"
                     /translation="MILQDFIALAPILDLGAFCIITMLAIAFRRHHATIALFAFMSCL
                     LTLVTLPGAATATPRTVTALLVVDGYALLYMGLVLSTTMIVIGLSYRYLKIHFSEQEG
                     IEEYYLLLLLATFGGLVLTVSAHFVSFFLGLELLGLSLCSLIGYVRTRHHPLEAAVKY
                     LLLSISASAFLLFGMALLYFESGSMTFRGVGMVMKQVQTVPGLWLGGVVLVFVGVALK
                     LGLAPFHMWVPDVYQGAPAPITAYIATVSKGALVALLLRFVTDVGALELPSFVHLFGL
                     LAVVSMVTGNVLALFQQNVKRLLAYSSIAHVGYLLVALLAGGAVAAEAVTFYVIVYCA
                     MTLGAFGVVTVYSREGGDAERFEDYRGLFWTRPWLAAVLALSLLSLAGIPVTAGFIGK
                     FYAIAAGVDAGLWWLVLALIGNSVISLYYYLRLIVTLFDEAPAPATLPQGQAVQSAQA
                     AGWTAVFSLGLVASIILLLGVYPEPLM"
BASE COUNT          432 a          734 c          765 g          723 t
ORIGIN      
        1 ggtcggcttc tttcactgca acattctctt ggcgctcggt ggggtcatcg gggtgtttct
       61 ggcgatcgat ctgtttctct tttttttctt ttgggaggtc atgctggtcc ccatgtatct
      121 cctgatctcc gtgtggggcc atgccaatcg tcggtacgcc tcgttcaagt tcttcttgtt
      181 tacgcaggcg gggagcttgt tgctcttggt cgcgatcatc accttggcat ttcttcatca
      241 acaggcgact ggaaagccca gtttcgacta tgccgatctg atgggactga cgctgaacaa
      301 ggggatggcc tggtggctga tgctcgcgtt cttcatcggc ttcgtggtga agctgccggt
      361 tccgcctttt catacatggc tacccgacac gtacaccgaa gctcccaccg gtgcaagcat
      421 catcctcgcg ggactgttgg caaaaaccgg cgcctatgga ttactgcggt tcacggtccc
      481 gatgtttccg gaagtgatca gtgacttcgc tcccattgca atgggactcg gtgcgatggg
      541 cattctctac ggagctgtct tggcctgtgc ccaaacggat atcaaacgac tggtggccta
      601 cagcagcctc agccacatgg gatttatctt gctcggtgcc ttctccggga cggaattggc
      661 gttgcaaggg gcggtgatgc aactggtcgc gcatgggctg agcacaggcg ccctcttcat
      721 gctggttggt gcgctgtatg agcgtttcga gacgagagac atgcgacaga tgggaggact
      781 atggggagtg attccgcgtg gcgcctcgat ggcgctgttt tttgcctgtg cttctctcgg
      841 tcttccaggc ctgggaaatt tcatcggtga atttctcgtg ctgttcgggt cctattccgc
      901 tcagccgatg gtcgcgatcc tagcctcaat cggtatggtc atggccgcaa tctattccct
      961 cggcatgatg caacgcacct tctttggccg gcagcgtgaa actcggctct caccggacct
     1021 gtcgaatgtc gcgtttggaa ccctgctgct catagcggtc ttgcaggtct ggtttggtct
     1081 ctatccgagg ccggtattgt ccaccacgag gccggtcatg gaaacgcttg tccagccggc
     1141 actcccatta cccaatctga ctcattcgcc atcggaactc atgcagtcgt ccaacctctt
     1201 caccccgaac gtgagtaccc catgatcctc caggatttta ttgcccttgc ccctattctc
     1261 gacctcggag cgttctgcat catcacgatg ctcgccatcg ccttcaggcg acaccatgcg
     1321 acgatcgccc tctttgcatt catgtcgtgc ctgctcaccc tggtcactct gcccggggcg
     1381 gcaacggcga caccgcgaac cgtgacggcg ctgctggtcg tagatgggta tgccttgttg
     1441 tacatgggac ttgtgctgag cacgacgatg atcgtgatcg gactgtcgta ccgatattta
     1501 aagattcatt ttagtgaaca ggaaggcatc gaagaatact acctcctgct cttgcttgcc
     1561 accttcggtg ggttggtgct taccgtttcg gcgcacttcg tgtccttttt cctggggctc
     1621 gaactgctcg gtctttctct gtgcagtttg atcgggtatg tacgaacacg acatcacccg
     1681 ttggaagccg cagtgaaata tcttctgctt tctatctccg cgtcggcatt ccttttattc
     1741 ggcatggccc ttctgtattt tgaaagcggc tccatgacgt ttcgcggcgt gggcatggtg
     1801 atgaaacagg tgcagacagt tcctggactc tggctgggag gagttgtcct ggtctttgtc
     1861 ggcgtcgcat taaaactcgg gctggcgccg tttcacatgt gggttcccga tgtgtaccaa
     1921 ggagctccgg cccccattac ggcctatatt gccacagtgt ccaagggggc gcttgtggcc
     1981 ttactactac gcttcgtgac cgatgtaggg gcattggagc tgccatcgtt tgtccatcta
     2041 ttcggtcttc tcgccgtggt gtcgatggtc acgggaaacg tgcttgccct ttttcaacag
     2101 aatgtcaaac ggctcttggc ctattcctcg attgcgcatg ttgggtacct gctcgtggca
     2161 ctgttggctg gtggcgctgt ggctgcggaa gccgtgacct tctacgtgat cgtgtactgt
     2221 gccatgacgt taggagcctt tggcgtcgtg accgtgtact cacgtgaagg aggggacgcg
     2281 gaacgtttcg aggactatcg cggtttgttt tggacgcgcc cctggctcgc cgccgtcttg
     2341 gcactgagtc tgctgtcgct ggccgggatt ccggtgacag ccgggttcat cgggaaattt
     2401 tatgcgatcg ctgccggtgt tgacgccggc ctgtggtggc tcgtgttggc gttgattggg
     2461 aacagcgtca tcagcctcta ctattatctt cgactgatcg tgacgctgtt cgacgaggcg
     2521 ccggcaccag caacccttcc tcaagggcag gctgtgcagt cggcacaggc ggcaggctgg
     2581 acggctgtct tttctctggg gctcgtcgcc tcgataattc tgttgctagg agtctatcca
     2641 gaaccactga tgga
//
LOCUS       DATMTI010000056         5404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_214466, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000056 DATMTI010000000
VERSION     DATMTI010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214466"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VN039_00910"
     CDS             complement(<1..339)
                     /locus_tag="VN039_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44581.1"
                     /translation="MAIVNECVGLDIGQTGLKAVRFRRRLSGRETIDYFQHPMPFSRP
                     EDLEPARRVKSLRGFLWQNGLYGTDRLVTAIPCQDLFVRTLSFPFKDSTKLAQVVPFE
                     VENLVPMPLED"
     gene            complement(458..1363)
                     /gene="gspN"
                     /locus_tag="VN039_00915"
     CDS             complement(458..1363)
                     /gene="gspN"
                     /locus_tag="VN039_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HWV44582.1"
                     /translation="MGGDRRIMTIKWPEAWREILAWTVAGVTILALCLMATFPYGMLQ
                     ARVVAELSRATGMEIRVVDWTVGLPLGLEWRNVTLSQPNWTPLQLALLQAKLGVMNAL
                     GGTLGLDVVAKLNETASPPGLAKGTVTASSLSLASPVTIKGQVQHVDLSKVLRRYVTH
                     GTLSGDFSHRIDSSQTTATTMKGEGMWTAEATDLVVDQIPLGNGRILSLTFSKVTAGL
                     ACRDLRCDVTQLKGDGIDGSFTGEGYITIQQPLQQSQVNLTVTVIPGPGFASKAGTLG
                     LPSPPPGTAMTVKIVGTLAQARIAL"
     gene            complement(1339..1962)
                     /locus_tag="VN039_00920"
     CDS             complement(1339..1962)
                     /locus_tag="VN039_00920"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HWV44583.1"
                     /translation="MSLPAVSLFKSQAGFTLVEILVAVALLGVIGAMVFGSLVMTMNA
                     VDAGREHVAKEQAIRRILRLMAEEISLSKRNTMYPWVGTNATLDGQPADILALLAMSH
                     ELSTSAAKESETVRVVYTRERDRLIRFVRRNLYTLTDTNETLDQMELADRVQAFNIRY
                     YDDQNRIWIDEWPAASKLPKALLIEVTFQYPDAAPWTVREWVAIGAS"
     gene            complement(1934..2371)
                     /locus_tag="VN039_00925"
     CDS             complement(1934..2371)
                     /locus_tag="VN039_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWV44584.1"
                     /translation="MAGEGHRTKSDEDGFTLLEVLLAIALLAIALPILLGLRNFDLEL
                     QERASELTAATLLAQEKLLETELVGQYAIGESTGEFPNIPLGTQLTMEAVPRAVGYKW
                     KRTIAPTPLELIREIRIKVSWFRGGLEEAVEVSTYVFAGRLTF"
     gene            complement(2378..3052)
                     /locus_tag="VN039_00930"
     CDS             complement(2378..3052)
                     /locus_tag="VN039_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44585.1"
                     /translation="MVIGCWTLGIGHSVHARPLSPHASRLTPRPSLSSSGFTILEMLI
                     VLFLLTLVVVVVFPRFSLDEDLSSTGRKLVGVLRTFQELAATRQQPLKLYLDMDRGTY
                     WMMTVEDKEERLPLDPAWKLPRSLPESIRLTEVSVGQDKHFSGRVDISFFANGRIEPV
                     TMYLMDEKNNLLGLAVDSLTGGIRVSDERLEPPPNRIIPDRVRLLLKPNKQAGALPRG
                     LFPTQQ"
     gene            complement(3060..3623)
                     /gene="gspG"
                     /locus_tag="VN039_00935"
     CDS             complement(3060..3623)
                     /gene="gspG"
                     /locus_tag="VN039_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HWV44586.1"
                     /translation="MKRDKGGLTMTDPEKQCRPTTYIGERNRLLRFGAWLNRRTAASS
                     AGFTFIEIMVVVAILAILAALVVPRIMGRTDDAKRTAAKVQIRNIEGALQLYKLDNGV
                     YPTTEQGLKALIEKPSVGVVPKKWKIGGYLPKLPEDPWGNPYKYLSPVQKGEYKVEYE
                     ITSLGTDGEVGGEGVNADITNWNLDKE"
     gene            complement(3656..4948)
                     /gene="gspF"
                     /locus_tag="VN039_00940"
     CDS             complement(3656..4948)
                     /gene="gspF"
                     /locus_tag="VN039_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HWV44587.1"
                     /translation="MDYPAPIGQTRRLRSLLIMPVYQYQGYRNDGGAATGIIDAENVK
                     VARLKLRKEGVYPTDVVEQGQAPGRSREKTTGRTERSVGRSATLSSTDLALMTRQFAT
                     LLVAGLPLVEALGVLVEQAEKKPIKALLADVREQIRGGKALSAVLGTYEKDFSPIYVH
                     MVRAGEASGALDQILFRLAEFLDKQLALRNKVTNAMLYPIIMLVIGSVILFFLITFVV
                     PKITLVFAQQKQALPWPTVALMSISQFFADYWMMLVGFVLGGFYMTRRFIRTGAGRMT
                     ADRLILKFPLIGDVARMVSISRLTSTLSTMLASGVQLLDAMDVSKRVMNNRVLEETVE
                     TARQNIREGETIADPLKRSGEFPALVTHMIAVGEKSGEMEEMLRRVSQIYDGEVERVI
                     ARLTSLMEPIMILAMGAVVLFIVVAILLPIFEMGGMVH"
     gene            complement(4995..>5404)
                     /locus_tag="VN039_00945"
     CDS             complement(4995..>5404)
                     /locus_tag="VN039_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HWV44588.1"
                     /translation="MGIEPFLVASSVVAVLAQRLLRRICPDCKHPYRPSEEELSRLDL
                     APGSDATLYRGAGCAACSQTGYRGRTGIFELMVLDDEIRRLIGSKADSTAIKQAAIAK
                     GMVTLKQEGAERVIQGHTTLEEVMRITQQEIEV"
BASE COUNT         1078 a         1551 c         1559 g         1216 t
ORIGIN      
        1 atcttcgagc ggcatgggga cgagattctc gacctcaaag gggacgactt gagcgagctt
       61 ggtcgaatct ttgaagggaa aggacagggt tctgacgaaa aggtcctggc aaggaatcgc
      121 ggtcactaac cggtctgtgc catagagtcc gttctgccag aggaatccac gcagcgactt
      181 cacacgtcgt gccggctcga gatcctcggg gcgggaaaac ggcatgggat gttggaagta
      241 atcgatcgtt tcgcgcccgc tcaggcggcg gcggaaacgc acggctttca atcccgtttg
      301 gccgatgtcc agcccgacac attcgtttac aatggccatg catccgactt ccgtaggtgt
      361 ttcgtgcttt tggcttcggg tttcacgtgc gagtgaatag tatcaggtac actcaaattg
      421 caatctccaa cccgcaacct caaacgccag ccgtacttta caatgcaatc ctcgcctgtg
      481 ccaaggttcc gacgatcttg acggtcatgg ctgttcccgg aggcggcgag gggaggccca
      541 gtgtgccggc tttcgaagca aatccagggc cggggatgac cgtcaccgtg agattcactt
      601 ggctctgttg caggggttgc tgaatggtga tgtatccttc gcccgtaaag gagccgtcga
      661 tgccgtcgcc cttcagttgc gtcacgtcgc agcggaggtc gcggcatgcg agtcctgcag
      721 tgaccttgct gaatgtgagt gacaggattc gtccgtttcc aaggggaatc tgatcgacca
      781 ccagatccgt cgcctcggcc gtccacatgc cttccccctt catcgtggtg gcggtcgtct
      841 ggctcgagtc gatccgatgg gaaaaatctc cgctgagcgt gccgtgcgtg acgtagcggc
      901 ggaggacttt ggaaaggtcc acgtgctgaa cctgcccttt gatcgtgacg gggcttgcca
      961 aggaaagaga tgaggcggtg acggtgcctt tggcgagacc tggcggggaa gcagtctcgt
     1021 tcagctttgc gacgacatcg agtccaagcg tgccgcccaa cgcgttcatg actccgagct
     1081 tggcctgcaa cagagccaat tgaagcgggg tccagttcgg ttgcgacaag gtgacgttcc
     1141 gccattcaag gcctaatgga agtcccaccg tccaatcgac aactcggatc tccatgccgg
     1201 tggctcgtga gagttccgca accactcgcg cttgcagcat gccgtatgga aaggttgcca
     1261 tcaggcacaa cgccaagatg gtgacgcccg ctaccgtcca agccaagatc tctcgccatg
     1321 cctcaggcca tttgatggtc atgatgcgcc gatcgccacc cattcccgca ccgtccaggg
     1381 ggcggcgtcg ggatattgaa acgtcacttc gatcaacaag gccttgggga gttttgaggc
     1441 cgccggccat tcgtcgatcc agatccggtt ctggtcgtcg taatatcgga tattgaaggc
     1501 ctggacacga tcggccagtt ccatttgatc caatgtctca ttggtatcgg tcagcgtata
     1561 gagatttctg cggacaaacc gaatcagccg atcacgttca cgtgtgtaga ccacgcgaac
     1621 ggtctcgctt tccttggcgg ccgacgtact caattcgtgg ctcatcgcga ggagcgccag
     1681 gatgtcggcc ggttgcccgt ctagcgtcgc gttcgttccc acccagggat acatggtatt
     1741 gcgtttgctg agggaaattt cctctgccat gaggcgcaaa attcttctga tcgcctgttc
     1801 cttggccaca tgctctcgcc cggcatccac ggcgttcatc gtcatgacga gcgaaccaaa
     1861 gaccatggcg ccgatcacgc ccaacaaggc cacggcgacc aagatctcga cgagcgtaaa
     1921 gcccgcttgg gatttaaaaa gtgagacggc cggcaaagac atacgtgctc acctccaccg
     1981 cttcttccag tccgccccga aaccacgaga ccttgatccg aatctctcga atcagctcca
     2041 gcggagtggg tgcgatcgta cgcttccatt tgtacccgac cgctcgcggg accgcctcca
     2101 tggtcagttg tgttccaagc gggatgttcg gaaactctcc ggtgctttcc ccgatggcat
     2161 attgtcctac gagctctgtt tccagtagtt tctcttgagc cagcagcgtg gcggccgtca
     2221 attcggacgc tctctcctgg agctccagat cgaaatttct aagaccaagg agaatcggga
     2281 gcgcgatggc cagcaaggcg atggccagca gtacttcgag cagcgtgaat ccgtcttcgt
     2341 cggatttggt tcggtgtcct tctccagcca tcgcgatcta ctgttgagtt ggaaacaacc
     2401 ctctcggcag tgctcctgct tgtttgttcg gcttcaagag gagtcggact cggtcgggga
     2461 tgatccggtt cgggggaggt tcgagccgtt cgtcgctgac tcgaatccct cccgtcaatg
     2521 aatcgaccgc cagtcccaag agattattct tctcatccat caggtacatc gtgaccggct
     2581 caatccgccc gttggcgaag aaggatatgt cgactcgccc ggaaaaatgt ttgtcttgtc
     2641 cgacggagac ttccgtcaat cgaattgatt caggcagcga acgaggcagc ttccatgccg
     2701 gatcgagcgg aagccgttct tctttgtcct ccaccgtcat catccagtag gtcccccgat
     2761 ccatatcgag gtagagtttc aggggttgtt gtcttgtcgc cgccagttct tgaaatgttc
     2821 gaaggacacc gacgagtttc cgtccggtgg agctcaggtc ctcgtcgagg ctgaatcgag
     2881 ggaaaacgac caccacgacc aaggtcagga gaaacaggac gatcagcatt tccaggatgg
     2941 tgaagcccga tgaagagagt gaggggcgag gggtgaggcg cgaggcgtga ggggagagcg
     3001 gtcgcgcgtg aaccgaatgc ccgatgccca atgtccagca gccgatgacc attgacttcc
     3061 tattccttgt cgaggttcca attcgtgata tcggcgttca cgccctcacc gccgacttcg
     3121 ccgtccgtcc cgagggaggt gatctcatac tccactttgt actctccttt ttggaccgga
     3181 ctgaggtact tgtacggatt gccccaagga tcttccggaa gcttcggcaa gtatcctccg
     3241 attttccatt tcttcgggac cacaccgacg gagggctttt caatgagcgc cttgaggccc
     3301 tgttccgtgg tggggtagac cccgttgtcc aatttgtaga gttgcagcgc tccttcgata
     3361 tttctgatct ggactttggc cgccgtgcgc ttggcatcat ccgtcctgcc catgatgcgc
     3421 gggacgacca gggccgcgag aatggccaag atggccacga cgaccatgat ttcaatgaac
     3481 gtaaagccgg cggagcttgc tgctgtccgc cgattcagcc aggcgccgaa gcgaagcagt
     3541 cgattgcgtt caccgatgta tgtggtaggc cggcactgct tttccggatc agtcatcgtg
     3601 agtccccctt tatctcgttt cacgctcttc ctctaccatg tttcgtatcg tctccttagt
     3661 ggaccatccc ccccatctcg aagatgggta acaggatcgc gacgacgatg aagaggacga
     3721 cggcgcccat cgcgaggatc atgatcggct ccatgagcga ggtcaagcgg gcgatgaccc
     3781 gctccacttc accgtcgtat atttgactca cccggcgcaa catctcctcc atttcaccgc
     3841 tcttttcacc gacggcgatc atgtgggtga ccagggcggg aaattcgccg ctccgtttta
     3901 acgggtcggc gatcgtctct ccctcgcgga tgttctgtcg tgcggtttcg accgtttctt
     3961 caagaaccct attgttcatg acgcgcttcg agacatccat cgcatcgagc aattgcactc
     4021 cgctggccaa catcgtggac agggtgctgg tgagtctgga aatcgacacc atccgtgcga
     4081 cgtctccgat caatggaaac tttaatatca accggtccgc cgtcatgcga ccggcgccgg
     4141 tccggatgaa gcgccgcgtc atatagaagc ctccgagcac gaacccgacc aacatcatcc
     4201 aatagtcggc aaagaactgg ctgatcgaca tcaacgccac cgtcggccag ggcaacgctt
     4261 gtttctgttg agcgaacacg agcgtaatct tgggaacgac aaaggttatg aggaaaaaga
     4321 ggatgaccga tccgatcacg agcatgatga tggggtagag catcgcattg gtgaccttgt
     4381 ttctcagggc gagttgttta tccaagaact ctgcgaggcg gaacaagatc tgatccagcg
     4441 ctccgctggc ctcgccggct cgtaccatgt gaacatagat gggggaaaag tccttttcgt
     4501 acgtccccaa gacggcactc aacgcttttc ccccgcggat ttgctctcgg acatcggcga
     4561 ggagggcctt gatgggtttt ttctcggctt gttccaccaa gacgccgagg gcttcgacca
     4621 acgggagccc ggccacgagc aatgtcgcaa actgtctggt catcagggcc aggtcggttg
     4681 agctgagggt ggccgatcgg ccgacggatc gctcggtgcg gccggtggtt ttttcccgcg
     4741 atcggccagg cgcttggccc tgctcgacga catcggtcgg atagacgcct tcttttctca
     4801 gtttgaggcg agcgactttg acgttctccg cgtcgatgat cccggtcgcg gctccgccgt
     4861 cgttccggta gccctggtat tgatagaccg gcatgatcag cagactccgg aggcgtcggg
     4921 tttggccgat cggtgccgga tagtccaaga tcgaaccgac tgagaggagc ggacagcgcg
     4981 tcggtgccgc atcattagac ttcgatttcc tgttgagtga tccgcatgac ttcttccaac
     5041 gtggtatggc cttgaatgac tcgctcagcc ccttcttgtt ttaacgtcac catgcccttg
     5101 gcgatggctg cctgtttgat ggcggtggag tcggctttgc ttccgatgag ccttcggatc
     5161 tcgtcatcca gaaccatgag ctcaaaaata ccggtacggc ctcgatagcc ggtttgggaa
     5221 caggctgcac agccggctcc tcggtagagg gtcgcgtcag aaccaggcgc gaggtccaag
     5281 cggctgagtt cttcttcgct cggacgatag ggatgcttgc agtccggaca gatccgcctc
     5341 agtagccgtt gggcgagaac ggcgacgacc gatgaggcca ccagaaaagg ctcgattccc
     5401 atat
//
LOCUS       DATMTI010000057         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_86996, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000057 DATMTI010000000
VERSION     DATMTI010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86996"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /gene="dapF"
                     /locus_tag="VN039_00950"
     CDS             <1..914
                     /gene="dapF"
                     /locus_tag="VN039_00950"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWV44589.1"
                     /translation="GNHYQFTGLHREIGMKNGFFRGHGLGNDYLVVDPKEMTFKLTPK
                     NIKAICDRNWGAGSDGILAFVPSKRADFGLRIFNPDGSEAEKSGNGLRIFARYLHATG
                     KTRKTRFTVETKGGLASIALHIDRHGDAAAATVEMGVATFKPDALPCSLNVPELVQQS
                     IQAAGRTLTFTGVSVGNPHCVVFKSAGESWSREDLLALGPALENHRLFPKRTNVQLAV
                     PTGPKEIFILIWERGAGETQASGSSSCAAASAAVRVGLVRSPVTVKMPGGVLHIDVAP
                     DFSLTMKGPVAEVARGTLSPSFVRGLR"
     gene            982..>2660
                     /gene="uvrC"
                     /locus_tag="VN039_00955"
     CDS             982..>2660
                     /gene="uvrC"
                     /locus_tag="VN039_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWV44590.1"
                     /translation="MTSDLHSKLAHLPGNPGVYLFKNAQGEIIYIGKAAVLADRVRSY
                     FQKGADHSPKTSLLVGHITDVETMVTRSELEALILESNLVKRHKPRFNIVLRDDKQYP
                     YVRLPVKDDFPRLSIVRRVQKDGALYYGPYTPANALRETLKVIKHVFPLATCTIDIDG
                     TADRACIEFEIKRCMAPCTGNQSMEEYHQIVKQVRQFLEGRDRELLDDLRTRMEAAAE
                     REEFEEAARLRDRLFKIERMLEKQRITQTSATDQDVIGLARQGSAVDLQILFVRGGLL
                     IGRKDFFWPQSADAPDTELVRSAIEQFYNKDGQPPREVLVPTDLDDIALIEQWLSDKR
                     GESVRILAPERGAKHQLVLLAEENAAAAAADHLRDEELDRQAGEELKRLLRLNHAPRR
                     IEGFDISNTMGNQSVASMVVWEDGQMKKADYRRFKIQTVTGANDFASMKEVVTRRYGR
                     EENLAQPDLILIDGGLGQLAAALEGLKEAGRQGLPILGLAKARGDKEERIFLAGRKNP
                     IVLKPQSPSTHLLQCIRDEAHRFAITFHRKLRGKSLVASRLDQVIGIGEIRR"
BASE COUNT          616 a          763 c          772 g          509 t
ORIGIN      
        1 ccggcaacca ttatcaattc acaggacttc acagggagat tggcatgaag aatggtttct
       61 ttcgcggaca tggtctcggc aatgactatc tggtagtgga tcctaaggag atgaccttta
      121 aactgactcc caaaaacatc aaagctattt gcgaccgcaa ttggggcgca ggcagtgacg
      181 ggattttggc cttcgtccct tccaagagag ccgatttcgg gctacgcatt ttcaacccgg
      241 acggcagcga ggcggaaaaa tcaggcaacg gcctcaggat ctttgcacgc tatctccacg
      301 cgaccggtaa aacgaggaag acgcgcttca cggtcgagac gaagggcggc ctggccagca
      361 ttgcgctaca tatagaccgg catggtgatg cagccgccgc gaccgtcgag atgggagtcg
      421 ccacgttcaa gccggatgct ctcccctgtt cactgaacgt gcccgagttg gttcagcagt
      481 cgatccaggc agccggacgg accctgacat tcaccggcgt gagcgtcggt aatccacatt
      541 gcgtcgtctt caagtcagcc ggagaatcct ggtcacggga agatctcttg gcgcttggac
      601 cggccctgga aaaccacaga ctatttccta aacggactaa cgtgcagctc gcagtcccaa
      661 ccggccctaa ggaaatcttc attctgattt gggagcgtgg cgccggcgag acgcaggcct
      721 ccggctcttc gtcctgcgcc gcagccagcg cagcggtacg cgtaggactc gtgagaagcc
      781 ccgtcaccgt gaagatgccg gggggggtgc tgcatatcga cgtggccccg gacttcagcc
      841 tcaccatgaa agggccggtg gccgaagttg cgcgaggcac gttgagcccg tcgttcgtgc
      901 gagggctacg atagagagtc gtcgagctgt acaatatggc catgttgttc ttctctgcca
      961 atgactaacg accgtgacca aatgaccagt gatttacatt ccaaactcgc tcaccttccc
     1021 ggcaatcccg gcgtctatct gttcaagaac gcgcaaggag aaatcattta catcggaaaa
     1081 gccgcggtac tcgccgaccg cgtccgatcc tatttccaga agggtgcgga ccatagcccc
     1141 aagaccagtc ttctcgtcgg ccacatcacc gacgtagaaa cgatggtgac tcgatccgaa
     1201 ctcgaagccc tgattcttga gagcaatctg gtcaagcgcc ataaaccccg cttcaacatc
     1261 gtgctgcggg acgacaagca gtatccctat gtgcggctgc ccgtcaaaga cgattttccc
     1321 cgactctcga tcgtgcgccg cgtgcaaaag gacggagctc tctactatgg cccttataca
     1381 ccggcgaacg cacttcggga gacgttgaaa gtcatcaagc acgtctttcc cctcgccaca
     1441 tgcacgatcg atatcgacgg gaccgccgac cgcgcctgca tcgaatttga gatcaagcga
     1501 tgcatggcgc cctgcaccgg caatcagtcg atggaagagt accatcagat cgtcaaacag
     1561 gtccgccaat ttctggaggg ccgggatcga gaattactgg acgatctccg tacccgcatg
     1621 gaagcggcgg cggagcggga agaattcgag gaagcagccc gactgcggga ccgcctcttc
     1681 aagatcgaac ggatgctgga aaagcagcgg atcacgcaga cttcggccac agatcaggac
     1741 gtgatcggct tagccagaca aggttcggca gtggacctcc agattctgtt cgtgcgcggc
     1801 ggcttgctga tcggacggaa agatttcttc tggcctcagt cggcggatgc accggacact
     1861 gaactcgtcc gctccgcgat cgagcagttc tacaataaag atggccagcc gccgagagaa
     1921 gtcttggttc cgaccgacct ggacgatatt gcgctgatcg aacaatggct ctctgataaa
     1981 cgcggggagt ctgtccgcat cctggctcct gagcgggggg ccaagcatca acttgttctt
     2041 ctggccgaag agaacgcggc tgccgcggcc gccgatcact tgcgcgatga ggaactggac
     2101 cggcaagccg gtgaagaact gaaacgattg ctgcgactga accacgctcc tcgccggatt
     2161 gaagggttcg acatctctaa cacgatgggc aaccaatcag tcgcctcgat ggtcgtgtgg
     2221 gaagacggac agatgaagaa ggcggactac cggcgattca agatccaaac agtcacaggg
     2281 gccaatgact ttgcaagcat gaaagaagtg gtcacgcgtc gctatggaag agaggagaac
     2341 ctggctcagc cggatttgat tctcatcgac ggcgggttag gtcaactggc tgccgcgctg
     2401 gaggggctca aagaggccgg acgacagggt ctgccgatcc tgggactggc gaaggcccgc
     2461 ggcgacaagg aggagcggat cttcctggcg gggagaaaaa acccgatcgt gctcaagccg
     2521 caatctcctt ccacccatct gctccaatgc attcgtgatg aagcccatcg ctttgcaatc
     2581 acgttccacc gaaagctccg cggcaaatca ctggttgcct cgagactgga tcaggtcatc
     2641 ggaatcggtg agattcggcg
//
LOCUS       DATMTI010000058         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_765832, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000058 DATMTI010000000
VERSION     DATMTI010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765832"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..228
                     /locus_tag="VN039_00960"
     CDS             34..228
                     /locus_tag="VN039_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44591.1"
                     /translation="MRGGPPGIPGKPRPALWKFGAKRLVVLGVIMLAEIPPALGAPAG
                     GTGEITTGDRGIGEIATGDC"
     gene            496..1623
                     /locus_tag="VN039_00965"
     CDS             496..1623
                     /locus_tag="VN039_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV44592.1"
                     /translation="MKRNNWVGSVLLLTLVILIGIGLAAWKYELIQNQQAASANQPEP
                     MESVTIAVARAIDHRQTTTSIGTVLALRSITLKNELAGTVREVRLTPGQIVEAGTLLV
                     VLDVSVEKAELQAQEAQAALAKTVLNRRQHLSEELASTKEEVDRARADLDVAQAQIAR
                     TKAIIAKKTIRAPFRARIGMADVHPGQYLDEGTLLTTLQGVGEAVHVDFTVAQQVAAR
                     LQVGETVEVFAAGDAPTVIAKIVALDARVDPITRNAMIRARIEGTRNVPAPGASVRVR
                     IPVGPEQSVIAIPVSALRKGPGGDQVFVIAPDKDGRNRVHTRQVESGPMFGDEIVIQA
                     GLTTGEQIAAVGSFKLRDGILIAVAESVENNSKENHETGKP"
BASE COUNT          477 a          590 c          591 g          368 t
ORIGIN      
        1 ggtggccgtg gatcctgagc ccccaagggg ctgatgagag gaggcccgcc cggcattccc
       61 ggcaaaccgc gtcccgcact atggaaattt ggcgccaagc gattggtggt gctcggggtg
      121 attatgctgg ccgaaatccc acccgcatta ggcgccccgg ccggcgggac cggagaaata
      181 accaccggtg atcgcgggat cggagaaata gccaccggtg attgctgacc cggaagcgga
      241 cccgatgcga gctgagcaga tccggtcgac cacatgccga acaggaaaca ggcggccgcg
      301 aacactgaca gtaataactt gatattcaca actctatcta agcatagcca ccgccatgcc
      361 gcgcctggtt gaactcctag cggatcaatg aagaagccat gagacacgga agctgggcgc
      421 caggaaagct tcggatctgg taccatgatc cgccgagttt atcagattgg gaggcgcttc
      481 agcgtcgtct aagtcatgaa gagaaacaac tgggttggat cagttttact gctcacgttg
      541 gtgattctca tcggcatcgg cttggccgcg tggaagtacg aattgataca gaaccagcag
      601 gcagcctcgg ccaatcagcc ggaaccgatg gagtcggtca ccatcgccgt cgcacgagcg
      661 atcgaccatc gccagaccac gacctcgatc ggaacggtcc tcgccttgcg ttcgatcacc
      721 ttgaagaacg agctggcggg gacagttcgg gaggtccggc tcacacccgg acagattgtc
      781 gaagccggca cactgttagt cgtgctcgat gtctcggttg agaaagctga acttcaggca
      841 caagaagccc aggccgcact cgccaagaca gtgctcaatc gcagacaaca tctcagcgaa
      901 gaactcgcct ccacgaagga agaagtggat cgcgcccgtg ccgacttgga tgtggcacaa
      961 gcccagattg cccgcaccaa ggccatcatc gccaagaaga ccatccgggc ccccttccgt
     1021 gcgcgcatcg gcatggcaga tgtccatccc ggccagtacc tggatgaagg gacgttactc
     1081 acgacgcttc agggcgtcgg cgaagcggtg catgtggact tcaccgtcgc gcagcaggtc
     1141 gccgctcgct tgcaggtcgg ggaaaccgtc gaagtgttcg cggccggtga tgcccccact
     1201 gtcatagcca agatcgtggc cctcgacgcg cgcgtcgatc cgataacccg gaatgccatg
     1261 atacgtgcca gaatcgaagg cacccgcaat gtaccggcac cgggcgcttc ggtacgcgtc
     1321 cggattccag tcggtcccga acagagtgtc atcgccatac cggttagtgc gttgcgcaag
     1381 gggccgggag gcgaccaagt attcgtcatc gcgccggaca aggacggccg caaccgagtc
     1441 catacccgcc aggtagaaag cggccccatg ttcggcgatg agatcgttat tcaagccgga
     1501 ctgacaaccg gtgagcagat cgccgcagtg ggctccttta agctccgcga cggtatcctt
     1561 atcgcggtcg cggagtctgt cgagaataac tcaaaagaga atcacgaaac cgggaagcct
     1621 taaaccgacc cacatgtcac agaacaacct ccgaacatcg ttcaccgacg tgagcctgag
     1681 gagctgatgt tgacaagagc gagggcgtaa actatggtaa aactaccgcc gataaaatcg
     1741 tgattctctg tgtgctttcg tgagctgaga cctcggaggg agtaatctct cccgcaaaac
     1801 caactcatct cctcatgcag cattttgatt gacctgaaac accggtcttg agaaaggaaa
     1861 aggctatgca ccaccggttg tcacgcaagt ctttgggcgc attgttggtt atcgtgctct
     1921 ctctcaccgg ttgtaccggg tggacctatg aacgtatgaa tgatccctcg cgggacacct
     1981 ggcagaaacc acaaggagtc gttgaaaagc tgtccatcgc gcgagg
//
LOCUS       DATMTI010000059         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_384028, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000059 DATMTI010000000
VERSION     DATMTI010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384028"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..413)
                     /locus_tag="VN039_00970"
     CDS             complement(6..413)
                     /locus_tag="VN039_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44593.1"
                     /translation="MASKTLVHRRYRFWAPLAVFISFLFTPLVGANLLEMADLMAHPE
                     VYDHKTVVVIGLVKDVQLVIDQKGEPTFKFLLADDDGMIKVTSRIQVRNGDQVIVEGM
                     FTRRRQGGRLTVYSEVNAFTIRPLNQFHSDLVG"
     gene            complement(490..786)
                     /locus_tag="VN039_00975"
     CDS             complement(490..786)
                     /locus_tag="VN039_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44594.1"
                     /translation="MPSHHRLEKKLPHEVDITGIAVALAFLVIVGVFAGGRGNRTVSE
                     DGPPPPVSMQVAPTLAIEETEALQQPQDKPDLSDTSLTLSVPAESRSAHHSVTP"
     gene            complement(862..>1638)
                     /locus_tag="VN039_00980"
     CDS             complement(862..>1638)
                     /locus_tag="VN039_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HWV44595.1"
                     /translation="IAQKDIVSLYHDRLVDEKEVTWDDALRAVRAVRRRRLGAMILVE
                     EAVSSPDVNEVKSALLQGLHKCGLEVLSFGHLLQQWRARVMWLRRIDDPRSEWPDLSD
                     EALLQTLDQWLGPYLTGITTLDRVKRLDLTTPLHALLTHEQLRRLDRLAPTHITVPSG
                     SRLPLDYEQPESPVLAVRLQEMFGCKDTPRIAGGKIPVILHLLSPAKRAVQVTQDLGG
                     FWQRSYQDVRKELRGRYPKHHWPEDPIGAAPTAKAKRRNR"
BASE COUNT          375 a          434 c          454 g          375 t
ORIGIN      
        1 ggagtttatc ctaccaagtc ggagtggaat tgattgagcg gccgtatggt gaatgcattc
       61 acttcgctgt acacggtgag gcggccgcct tgtcgacgcc gggtaaacat accttcgaca
      121 atgacttgat ccccattccg aacttggatt cgactggtca ctttgatcat gccgtcgtcg
      181 tcggccagta aaaacttgaa cgtcggttcg cctttctgat cgatgaccag ctgcacgtcc
      241 ttcaccaagc cgatgacgac cacagtctta tgatcataca cttcgggatg ggccatgaga
      301 tcggccatct caagtaaatt ggcgccgaca agcggtgtga acagaaagct aatgaagaca
      361 gccagtggcg cccagaatct gtaccgccta tgaaccaggg ttttgcttgc catgatcggg
      421 gcgtattgta ccgaagatca tggcaagtac aagatgggca atcaccttac tcaaaggtct
      481 ccagttctgt tacggggtga cggagtgatg ggccgaccgt gattcagcgg ggacactgag
      541 cgtgagcgat gtatcggaga gatcaggttt gtcttgcggc tgctgtagag cctcggtctc
      601 ctctatggcc aaggtcggtg cgacttgcat ggaaaccggc ggaggtggcc catcctcgct
      661 cacagtgcga ttgccccggc ctccggcaaa cacaccgacg atcacgagga atgcgagcgc
      721 tacggcaatc cccgttatat ctacttcgtg aggaagcttt ttctctaatc ggtgatgcga
      781 cggcatgagt tccgtttttc ctgagcatga ggttgtgtac tggatactct cctcacaccg
      841 aataagccac aaggtagaac cctagcggtt tctccgcttt gccttggcgg tcggcgcggc
      901 acctatcgga tcttcaggcc aatggtgctt gggatatcgg ccgcgtaatt ctttacggac
      961 atcttgatag gacctctgcc aaaagccacc aaggtcttgg gtaacctgca ccgcccgttt
     1021 ggcaggggac aggagatgca agatcacggg aatctttccg cccgctatac gcggcgtgtc
     1081 cttgcaacca aacatctctt gcaagcgtac cgccagcacc ggagactcag gttgctcgta
     1141 atcgagagga agtcgggaac cactcggaac agtgatgtgg gtcggggcga ggcggtcaag
     1201 acgacgcagt tgttcgtgcg tcagtaaggc atggagcggt gtcgtcagat ccagccgttt
     1261 cacccgatcg agggtggtga tcccggtcaa gtaaggacca agccactgat ccaatgtctg
     1321 aagcaatgcc tcgtcggaaa gatccggcca ttctgatcga ggatcatcaa tgcgtctaag
     1381 ccacatgacg cgagcacgcc actgttggag cagatggcca aacgaaagaa cctcgagtcc
     1441 gcatttatga agaccttgta aaagtgcgga ttttacttca tttacatcgg gtgatgagac
     1501 cgcctcctct acaaggatca tcgctccaag tcgtcggcgc cgtacggctc gaaccgctct
     1561 aagggcgtca tcccaggtca cttctttttc atcaaccaac cggtcatggt aaagggatac
     1621 aatatctttt tgagcgat
//
LOCUS       DATMTI010000060         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_44791, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000060 DATMTI010000000
VERSION     DATMTI010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44791"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..359)
                     /locus_tag="VN039_00985"
     CDS             complement(33..359)
                     /locus_tag="VN039_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV44596.1"
                     /translation="MPRVSKTIHKAEYPVFLELLIEARKGAGLTQVQLAAKTGLSQPY
                     VSAVERGGLRLDTLQLRAWLQACGSNLGAFGAELEDRLVMLERQRLDKKIKPSSRIKQ
                     KDKERL"
     gene            482..1255
                     /locus_tag="VN039_00990"
     CDS             482..1255
                     /locus_tag="VN039_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44597.1"
                     /translation="MNREGVAVGAIILGVVGGLVMYAAEHAALGLPFLALVAWGVYLF
                     IQARKNPKHGIRRVYRPESSYTVEEQSDGQLRFHVLVANQRSSAPVVAVLAGIMVACI
                     MGFITGIMVALNGSPFLLLLSPIAGVGIAIAILRSRADRRESVFDTRIYVSEVVINYP
                     DPAKDFALTTVPIGTIDRLRIQQSIGNKALMQSASASPGVTVQNWGERSKAKWSNWIA
                     DHSYTLEVHAQGKRHVLAGGMDEITADGLMQAVSRKIRL"
     gene            1285..1629
                     /gene="bamE"
                     /locus_tag="VN039_00995"
     CDS             1285..1629
                     /gene="bamE"
                     /locus_tag="VN039_00995"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HWV44598.1"
                     /translation="MKKIVFAVLAASLLSACVNTTTGVKMDTNKLAQLHVGTTTIAQA
                     EALLGQPTGIAKAPDGTTTLTYFYSQNGQDAKSYIPLLGNVLGKSTHESSMTYVQFDA
                     NGKYMKWWGGQG"
     gene            complement(1626..3260)
                     /locus_tag="VN039_01000"
     CDS             complement(1626..3260)
                     /locus_tag="VN039_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HWV44599.1"
                     /translation="MDIHAHARPHLEVHSRSKGHSSVAGCAYRLGLRLYDERAGVWHD
                     YRKRELGEEIVRALTVAPPGAPAWATDPAQLWNKVELAEKRKDAQVARDYRIPIPFGL
                     SDQDAGDLAEEMTRFICDELRVPVSLGLHRDADRDALGNLKPSHKQGFHAHLYFPTRP
                     LLDVADGEDGKGQTSMDFGPKLHEFSKQHLGSAFIELLNCRWSELATDYARKTGSSIT
                     YQFKSYKRLGVNITPQPALGRSATAMERRGLYTNRGDSLREALVMAQVFEKAHAGALK
                     AQRAQALQDVAREASKRSEAQPGPMLAVAPHALPLFGKRKASPTLQPFIPKIGSLAYR
                     LKASASSPKTKEEADELERSLVLVEALDKAFAVYHELMQQLDEAMKVIQVARAAKLDA
                     EFQADQSRQHRNRAKVRLSRWEEDHRFRLKMFASMGGSPLPEHEQLRGDVRRHDEHVQ
                     SLKQTIARHASEVVRLDVEASALKAKQADALSTIRNSLVKLHDQQSTLLPEIIKALPD
                     EDRALVQEQLPVLFPEVEKVDEATLSCGVAPSPKLG"
BASE COUNT          721 a          981 c          943 g          747 t
ORIGIN      
        1 agatgatgct agcggcctga cccttctgtt cctcaaagcc tctccttgtc cttttgcttg
       61 atgcgtgagg aaggcttgat cttcttatca agccgttgcc tttcgagcat caccaagcgg
      121 tcttcgagtt ctgctccgaa tgcacctagg ttgctaccac aggcttgcaa ccatgctcga
      181 agttgcaacg tgtccaaccg caacccaccc cgttcgaccg cagacacgta aggctgggac
      241 aggccagtct tggccgcgag ctgaacttgg gtcaggccgg ctcccttccg ggcctcaatc
      301 agaagctcca gaaagacggg gtactccgcc ttatggatgg tcttgctgac cctgggcatg
      361 gatgttcggc gcgctcacgc tgttcggtga agcgacgcta aacatcaaga tgagatactc
      421 catcatctta tacttcatct tgttatattc tgaccacctt aggcacggaa caggggaaca
      481 catgaaccga gagggagttg cggtcggcgc catcatcctg ggcgtcgtgg gtggcctcgt
      541 gatgtatgcg gcagaacatg ccgcccttgg attgccgttt cttgcccttg ttgcatgggg
      601 cgtttacctc ttcatccagg cacgaaagaa ccccaaacac ggaatccgac gggtctaccg
      661 acctgaaagc tcgtacacgg tcgaagagca gtccgatgga cagcttcgct tccatgtcct
      721 ggttgccaac caacggagta gcgcgcctgt tgtagccgtt ctcgccggca tcatggtcgc
      781 ctgcatcatg ggcttcatca ccggcatcat ggtggccctc aacggcagtc cgttcctgct
      841 tctgctctca cccatcgcag gggttggcat tgccatcgcc atcctgcgtt cgagggctga
      901 ccgtcgagag tcggtcttcg acacgcgcat ctatgtgtcg gaggtggtca tcaactatcc
      961 cgaccctgcc aaagacttcg ccctgacgac ggtgcccatc ggcaccattg accgccttcg
     1021 gattcagcag tcgattggca ataaagccct gatgcagtcc gccagcgcga gccctggtgt
     1081 gactgtccaa aattggggtg agcggtcgaa ggccaagtgg tcgaactgga tcgcagacca
     1141 ctcctacacg ttggaagtcc atgcgcaagg caaacgccat gtcttggctg gtggcatgga
     1201 tgaaatcacc gctgacggtt tgatgcaggc cgtcagccga aagattcgcc tctgacctca
     1261 actcaccaca acaggggaaa caccatgaag aagattgttt tcgccgtgct tgcggccagc
     1321 ctgctcagcg cctgtgtcaa cacgaccaca ggcgtgaaga tggacaccaa caagcttgcc
     1381 cagttgcacg ttggcacgac caccattgcc caggccgaag cccttctcgg gcaacccacc
     1441 ggcattgcca aggcccccga tggcaccacg accctgacgt acttctacag ccagaacgga
     1501 caggacgcga agtcctacat ccccttgctt ggcaacgtcc tcggtaagag tacgcacgaa
     1561 tcgagcatga cctacgtcca gttcgatgca aacgggaaat acatgaagtg gtggggtgga
     1621 cagggttaac ccagcttcgg agacggagcc accccgcagg acaaagtcgc ctcatccacc
     1681 ttctcgacct caggaaaaag gacaggcaac tgctcctgca ccaaggccct gtcctcatcc
     1741 ggcagggcct tgattatttc tggaagcaac gtggattgct ggtcgtgtag cttgaccaag
     1801 gaatttcgga tggtcgaaag cgcatcagct tgcttggcct tcaacgcact ggcttccaca
     1861 tccagcctga ctacctcgga agcatggcgg gcgatggtct gcttcaagga ctggacatgc
     1921 tcatcgtgtc ggcgaacatc accgcgaagt tgctcatgtt cgggaagggg tgaaccgccc
     1981 atgctggcaa acattttgag ccggaagcgg tggtcttctt cccaacggct tagccgaacc
     2041 ttggcccggt tccgatgctg gcgactttgg tcagcctgga actcagcatc gagcttggct
     2101 gcccgcgcaa cttgaatgac cttcatggcc tcatccagtt gttgcatgag ttcatggtag
     2161 acggcaaagg ctttgtcgag cgcttcgacc agcaccaacg accgttcgag ttcatccgct
     2221 tcttccttgg tctttgggga tgatgccgat gccttcaatc ggtacgcgag cgaaccaatc
     2281 ttggggatga aaggttgaag ggtcggagat gctttccgtt ttccgaacag gggcaatgca
     2341 tgcggtgcga cggccagcat cggccctggt tgggcttctg agcgcttcga agcctctctt
     2401 gctacatcct gaagtgcctg agcgcgctgg gctttcaagg cgccagcatg ggctttttca
     2461 aacacctggg ccatgaccaa agcttcacgc aaggaatcgc cacggttggt gtagaggcca
     2521 cgtcgttcca tggccgttgc tgacctgccc aacgccggtt gaggcgtgat gttcactccg
     2581 aggcgcttgt agctcttgaa ctggtaggtg atgctcgaac cggttttcct ggcgtagtcg
     2641 gttgccagct ccgaccaacg gcagttcaaa agttcgatga aggcggagcc gaggtgttgc
     2701 ttggaaaact cgtgaagctt cgggccgaaa tccatacttg tttgaccctt tccatcctct
     2761 ccatcggcaa cgtccaacag gggacgggtt ggaaaataca agtgagcatg gaaaccttgc
     2821 ttgtgtgatg gcttgaggtt gccaagcgca tcgcggtcgg cgtcacggtg caacccgagg
     2881 gaaactggaa cacgtagttc atcgcagatg aagcgggtca tttcctcggc caagtcgcca
     2941 gcatcctggt cgctcagacc gaaggggatg gggatacggt agtccctggc gacctgggca
     3001 tctttccgct tttcggccag ctcgaccttg ttccaaagct gagcggggtc ggtggcccat
     3061 gcaggggcac ccggtggtgc aaccgtcaac gctctgacga tttcctcgcc aagctctcgc
     3121 ttccgatagt catgccaaac accagcacgt tcatcgtaca aacgaagacc aagccggtag
     3181 gcacaaccgg caaccgaaga atggcctttt gagcgcgaat gaacttcaag gtgtgggcgt
     3241 gcgtgggcgt gaatatccat aggtgtcctc catgacgtct tagtgcttct gatcgggcca
     3301 atccgtgact ccattttgat tacttttgta aaagtaaaat caaaacgcca tcacatccaa
     3361 tcagattatc gagacctgtc aagcgcgaaa ag
//
LOCUS       DATMTI010000061         1967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_87178, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000061 DATMTI010000000
VERSION     DATMTI010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87178"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VN039_01005"
     CDS             complement(<1..474)
                     /locus_tag="VN039_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44600.1"
                     /translation="MAKVEYRRIPSHMVDYEELVSIGAIAIQALLKNKSEEEIERLNT
                     SYMATATRWAIRNELRSRYKWYTLKHNKGNEEEENADGTPALEGENIPAGMTTANAPT
                     SETVREAIYETILSIESIQEGNDGDAPYDFISDGSATPEESLEIVELGRAIKRAIE"
     gene            complement(605..1696)
                     /gene="flhB"
                     /locus_tag="VN039_01010"
     CDS             complement(605..1696)
                     /gene="flhB"
                     /locus_tag="VN039_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HWV44601.1"
                     /translation="MADENKQFEASQQKLRKAKEEGQVFKSKDLSTAAFLMFMFMILF
                     GLAPFVWKEMASLFILVFEQIPNKTIENIGWQYLALLTIRALAFLIGPFILLAALLGI
                     AGDFFQVGPLIATKAIEPKFDKLNPVKGFQNIFSQRTVIELVKNIIKMLILGYMAWIV
                     FQKYIGHFLAVGATENIFAILGVLGEVVMQFIFYAGGAFLAIGMADFLYQRWKYMQDQ
                     KMSFKEVKDEFKNTEGDPMVKHAIRQRRMQMLQQSMLEAVPTADVVTTNPIHIAVALK
                     YNSDVMEAPRVVAKGTELFAERIKEIARKNEVPVVENPVLARALFRLVEIDQEIPPDL
                     YQAVAEILMFAWRVKGQAPPVVGKPEQPE"
BASE COUNT          414 a          527 c          528 g          498 t
ORIGIN      
        1 ctcaatggcc cgtttgatgg cgcgacccag ctcaacgatt tccagacttt cctccggcgt
       61 ggcggagccg tcgctgatga aatcgtaggg tgcatcgccg tcgttgccct cttgaatgct
      121 ctcaatggac agaatagtct cgtagattgc ctcacgtacc gtctcagaag taggcgcgtt
      181 ggcggtggtc atccctgcgg ggatgttttc accttccaat gccggggttc cgtcggcgtt
      241 ctcttcttcc tcgttgccct tgttgtgctt gagggtatac catttgtagc gcgaacgtag
      301 ctcgttccga atggcccagc gcgtggcggt ggccatatag gaagtgttca gccgctcaat
      361 ctcttcttcg ctcttgttct tgagcaacgc ctggatggcg atcgccccga tgctcaccag
      421 ttcttcgtag tccaccatat gggaggggat gcggcggtat tccaccttgg ccacacgctc
      481 cacgaggggc aggtacttgg tgatattcac ttttccaggg acttttttag gtgatgtcac
      541 aatctttcgg tccagcattg ggttgtgtaa tccgaattag caaaaagtga tgcaggatga
      601 tgggttattc gggttgttcg ggtttgccga cgacaggcgg cgcttgtcct ttgactctcc
      661 aggcgaacat cagaatctcg gctacggcct ggtataagtc gggggggatt tcctggtcta
      721 tctctacaag acgaaacagc gcacgggcga gaacggggtt ttcaacgaca gggacttcat
      781 tcttacgcgc aatctcttta atccgttcgg caaaaagctc ggtgcctttt gccaccactc
      841 tgggcgcctc cattacatct gaattatact ttaacgcaac cgcgatgtgt atcggattgg
      901 tcgtaactac atcagctgta ggaaccgcct ccaacatact ttgttgcagc atctgcatcc
      961 gacgctgacg gatggcgtgt ttgaccatcg gatcgccttc ggtgttcttg aactcatctt
     1021 tcacctcttt gaaggacatt ttctgatcct gcatatactt ccagcgctgg tagaggaagt
     1081 cggccatccc gatggctaaa aacgccccgc ccgcataaaa gatgaactgc atgaccactt
     1141 cgcccagcac gcccaggatg gcgaagatgt tttccgtggc gcccacggcc aggaagtgcc
     1201 cgatgtactt ctggaagacg atccaggcca tgtagcccag gatcagcatc ttgatgatgt
     1261 tcttgaccag ctcaatgacc gtccgttggg agaagatatt ctggaagccc ttcacggggt
     1321 tgagtttgtc gaacttcggc tcaatggcct tggtggctat caggggacca acctggaaaa
     1381 agtcccccgc aatgcccaag agggcggcca acaggataaa cgggccaatc aggaaggcca
     1441 aggcccggat tgtcagtaac gcgagatact gccagccgat gttttcaatg gtcttgttgg
     1501 gaatctgctc aaacaccagg atgaacagcg aggccatctc cttccagacg aagggagcca
     1561 gcccaaagag gatcatgaac atgaacatca ggaacgcggc ggtggacagg tccttgctct
     1621 tgaagacctg accctcttcc ttggcttttc tcagcttctg ttgagacgcc tcaaactgct
     1681 tattctcatc ggccaacgcc gaacgtcctt atgtttattg cctgatcacg gcctgtggag
     1741 cggtataaac gggtgaatag ccaattggat cgaaccgggc gatagtgggt ggcgcttttc
     1801 aggctgctgc tccagcttct cgtcagcctc ctcgcaaagc catcccagtc ggataggcac
     1861 gggaggatag tcagcgggag cgtattgcgg gctatgcggg acgatcagct tattttctta
     1921 agctgttttc tctagcccaa ccctaattgc ctcaagttct attataa
//
LOCUS       DATMTI010000062         6726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_511689, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000062 DATMTI010000000
VERSION     DATMTI010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511689"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VN039_01015"
     CDS             <1..213
                     /locus_tag="VN039_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44602.1"
                     /translation="PVWKSFIQSLGRQKNVTMREFEQAASQVLGLTHIEKTIAISAFR
                     QQQEQRSAPPSHGPTAFRSERHLSVV"
     gene            412..720
                     /locus_tag="VN039_01020"
     CDS             412..720
                     /locus_tag="VN039_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44603.1"
                     /translation="MPLLSLQSPISDAQTAPPLYPALSQAAVAALWKGRVTEAIQMVR
                     LEQNIGLAEATDLIDTFLRSQPALKSQINEVQADAREGLLRWLIFLLTGGVGLAHFLD
                     "
     gene            741..941
                     /locus_tag="VN039_01025"
     CDS             741..941
                     /locus_tag="VN039_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44604.1"
                     /translation="MEAGQTLLALIGACTSFVTFCLMRFPQTRRSRFQRYGPFSLIAS
                     FFGALTILLISALTVYAQAVWH"
     gene            1438..1962
                     /locus_tag="VN039_01030"
     CDS             1438..1962
                     /locus_tag="VN039_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWV44605.1"
                     /translation="MQPRGYEPGSVTRFLAEDHRRLEHLLQTALASADHVVQGTYDHF
                     RAGLLRHIGMEEKILLPAVQRWNGGRPLSIAAKLRLDHGALAALLMPTPTTDIIGTIH
                     RILEQHNLAEEGPDGWYEICDRLAGAEADQLMTRLRATPEVAVLPHSDTAAVLSAVHR
                     ALERAGHTLGSGSR"
     gene            2105..3709
                     /locus_tag="VN039_01035"
     CDS             2105..3709
                     /locus_tag="VN039_01035"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HWV44606.1"
                     /translation="MTTETTPLSELAAAIARRRTFAIISHPDAGKTTLTEKLLLYSGL
                     IRTAGMVRGRKGGKTTASDWMGMEQERGISITASAMQFPYKDAVVNLLDTPGHQDFSE
                     DTYRTLTAADSAIMVIDAAKGVETQTRKLFAVCRMRRIPVLTLINKMDLPGRPPLDLM
                     TEVEQALDIHASAINWPIGSGSDFIGIVSRTDNRVQLFSKTMRGGATKVETNTLPLAE
                     LHSNGRVSQEAMAQVQHDLELLEIAGNPFTREQFLQGAVTPVFFASALTNFGIESFLD
                     TFVDLAPSPGMRPADSDDGSELFINPTNMPFSAYVFKLQANMNPKHRDSTAFLRICSG
                     RFERDMVVKHHRLSRDIRLARPHSLVAQERSTVEEAYPGDIIGIINPGVFAIGDTVSL
                     TGGFNFKPLPQFQAELFARLRPTDVGKRKAFDKGLSQMAQEGTVQIMRSLNDQDALIA
                     AVGRLQFDVLQYRLRHEYRVETIMDTLPFTCSAWLDGDPSTFKSPSASMMVKDQRGRV
                     VVLFGDQLMKTIARDRNPDHDLREMG"
     gene            3863..4132
                     /locus_tag="VN039_01040"
     CDS             3863..4132
                     /locus_tag="VN039_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HWV44607.1"
                     /translation="MDEQLRNQLARHRTELANERTLLAYIRTALGFVIVGVPAMWLLD
                     HPYMQTLGALALAAGVICLALGISRFVTVKAMIAQEFGSQVDKAA"
     gene            complement(4129..4725)
                     /locus_tag="VN039_01045"
     CDS             complement(4129..4725)
                     /locus_tag="VN039_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV44608.1"
                     /translation="MDQAHELDDLMEKMVATYVARNRAAGVLKVMLDETGIGFSPVID
                     HVTIRTLDIDRRAQPFVKLGYAYDETLQYDDWYAKVYRKAGYPALFVDQAYPDARGKT
                     SIIPGWVDRFGDKVFHHVAVRVEDIEQAVARLQQKGVVFAGKIVGASGGPLRQIFSSP
                     ETIDGQPFSVLELAERHRGYQGFLPPQADSLMKSSTGR"
     gene            complement(4740..6269)
                     /locus_tag="VN039_01050"
     CDS             complement(4740..6269)
                     /locus_tag="VN039_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWV44609.1"
                     /translation="MSTTHTALRDLGIQAVNSGGSTGTGWWSGRNDGSLLPSINPSTG
                     EQIAGVYPCSSDDYQRIVKESVEAFRTWRMVPAPKRGEMVRLIGQALREKKDQLGTLV
                     SLEVGKIKAEGDGEVQEMIDMADFAVGQSRMLYGVTMQSERPAHRMSEQWHPLGPVGV
                     ITAFNFPVAVWAWNAFVAAVAGDTVIWKPSPKAPLCAVAVQQICNRVMQQQGYSGIFS
                     LCIADQPALAEQMVQDERLPLISFTGSVPVGRRVASLVGQRLGRTLLELSGNNAVIVD
                     QTADLDMALRAILFGAVGTAGQRCTTTRRLIVHESRYEELVGRLVKAYAQVQIGNPLE
                     KDMLMGPLIDHVAVEGYRVALDEIKKEGGEILCGGHVLNRPGYFVEPTIVRAQNHWPI
                     VQRETFAPILYVMTFQTIDEAIQMQNSVPQGLSSAMFSNDVRHSERYLSVAGSDCGIA
                     NINIGTSGAEIGGAFGGEKDTGGGREAGSDSWKAYMRRQTNTVNWGTELPLAQGIKFG
                     S"
     gene            complement(6266..>6726)
                     /locus_tag="VN039_01055"
     CDS             complement(6266..>6726)
                     /locus_tag="VN039_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HWV44610.1"
                     /translation="YEAFNTSGGLGTLADSYARKVRTMNYKTLRYPDHCEKIHLLMKD
                     LKLNEDRETLKRVLEHAVPQTLQDVVLIYASVTGQNADGFFEENYVKKIYPQCIKGKL
                     WSAIQVTTASSACCVMDLVLTHPSAYHGFVTQESVLLKDFLDNDFGACFR"
BASE COUNT         1478 a         1879 c         1827 g         1542 t
ORIGIN      
        1 ccggtctgga aatcatttat tcagtcgctc ggccgtcaga agaatgtaac gatgcgtgag
       61 tttgaacaag cggcttctca agttctcggt ctgacacaca tcgaaaaaac aatcgccata
      121 tccgcattcc gtcaacaaca agaacagaga tcagcgccgc cttcccacgg tcctaccgcg
      181 tttcgcagtg aacgccacct gtctgtcgtg tgaccgacta ccgatctaca caacgataga
      241 gacgatacgg tgatgccttg atctcgtgcg gctaccgact gcgtgctcct gggaataacc
      301 ggcccgcgaa aggcctagct gaactcgagg gacgttgcgg gtttttcatt cgtcaattct
      361 tcggtttttc ttcggctgct cggacgatgg tgctaggctg tgtttcagcc tatgccgctt
      421 ttatctcttc agtctccaat ctctgacgct cagacagctc ctccgctgta tcctgcactc
      481 tcacaagcgg ctgtggctgc actttggaaa ggccgggtta ctgaagccat ccagatggtt
      541 cgccttgagc agaacatcgg tttagctgaa gccacggact tgatcgatac atttctccgg
      601 tcacagccgg ctctcaagag tcaaatcaac gaggttcaag ctgatgcgcg agaaggattg
      661 ctccgctggc tgatcttcct gttgaccggt ggtgtagggc ttgcacattt tttggattaa
      721 gtaatggaag gattggcaca atggaagctg gtcaaactct gctggcattg atcggagcct
      781 gcacatcatt cgtgacattt tgtctcatgc ggttcccgca gactcggcga tctcggttcc
      841 aacgctacgg tccattctcg ctcatagcct ccttcttcgg tgccttgacg atcctcctca
      901 tctccgcctt gacggtctat gcacaagctg tctggcactg aaattttctt cctcctccga
      961 caaggtgtac aatctacaaa ggtaagtcgt tgctcaaggg atacctatgt tcgctacgtc
     1021 cgagagttgg ctcggcatcg gaatcatcgt ggttatcggt ttaatggttg taggacttgc
     1081 cacttgcatg gggaagttgg tcatctggct tttcggaacg gggcaagaag ccgtagaaaa
     1141 catctggcag gggtaatgtg gcgataaagc tccatgaagc cgcataatca acagtcatgt
     1201 ttatcctgcc tcccactgtc aaggttcatc gcagcgttat cgaaaccatg acatcgagta
     1261 tagtttccac ctggacgccg gcatgtcgat ctagcattgt agggtttgca gtttatccgc
     1321 agtcagcctt gtaccagccg atatcttgtg tttttcgttc gaatccgtca tgaatatcct
     1381 caagctatgg tgcgagatcc ctctgtcggg tacacagctt tctcatataa aacggtgatg
     1441 caaccacgag gatatgaacc agggtccgtt acacgctttc tcgcggagga tcaccggcgc
     1501 ttagagcacc ttcttcagac agcccttgct tccgcggacc acgtggtgca gggaacgtac
     1561 gatcactttc gagccggcct gctccggcat atcgggatgg aagagaagat tcttctgccg
     1621 gcggtacagc gatggaacgg aggaaggccg ctttcgatcg cagcaaagct tcggctcgat
     1681 catggggccc ttgcagcctt actcatgccg actcccacaa ctgatatcat aggcacgatc
     1741 catagaattt tggagcagca taaccttgcc gaggaaggtc ctgacggttg gtacgaaatc
     1801 tgtgatagat tggcaggggc cgaggctgat caactgatga ccaggcttcg tgctaccccg
     1861 gaggtggcgg tgctcccgca ttccgatacc gcggccgtcc ttagtgcagt ccatcgtgca
     1921 ttggagcggg ccggtcatac gttgggatcg ggttcccgct gaacaacgga gtcaggaaaa
     1981 ggttcaagga aattccaatc gttgttcgaa ctcgaatcaa ccggaccgac tcaacggctc
     2041 gactcactca ccgtgtgaga atcatcccct tttaaacacg atatggtgtg ccgggcctcg
     2101 catcatgacg actgaaacga ctccactcag cgaacttgcc gccgccatcg cacggaggcg
     2161 cacctttgcc atcatcagcc atccggatgc cggtaaaacg acgctgaccg aaaagcttct
     2221 cttgtactcc ggcctgattc gaaccgcagg catggtgcga ggacgcaagg gcggcaagac
     2281 caccgcgtcg gattggatgg gaatggaaca ggagcgcggc atttccatca cggcgtcggc
     2341 catgcaattt ccctataaag acgccgtggt gaacttactg gatacgcccg gccatcaaga
     2401 tttttccgaa gatacctatc gcaccttgac ggccgcagac agtgccatca tggtcatcga
     2461 tgcggccaaa ggcgtggaaa cccaaacacg caaactattc gccgtgtgcc gcatgcggcg
     2521 gatccccgtc ctgacgttga tcaacaaaat ggacctgccg gggcgtccac cgctcgactt
     2581 gatgactgaa gtggaacagg cattggacat tcacgcaagc gcgatcaact ggccgatcgg
     2641 gtcgggcagt gacttcatag gcattgtcag ccgtaccgac aaccgggtac agctgttcag
     2701 caaaaccatg cgtggcggag cgaccaaagt ggagacgaat actttgccct tggctgaact
     2761 ccattcaaac ggtcgagtct cgcaggaggc gatggcacag gtgcagcacg atttggagct
     2821 tttggagatc gccggaaatc ccttcacgcg ggagcaattt ctgcagggag cggtcacccc
     2881 agtctttttc gcatccgcgc tcacgaattt cggcattgaa agctttctcg atacgtttgt
     2941 agatctggcg ccaagtccag gtatgcggcc tgccgacagc gatgatggtt ccgagctttt
     3001 catcaatccg acgaacatgc cgttcagcgc ctatgtgttc aaactgcaag ccaatatgaa
     3061 ccccaaacat cgagacagca ccgcctttct tcggatctgt tccgggcgtt tcgaacgaga
     3121 catggtggtc aagcatcacc gattgagtcg cgatattcga ctggcgagac cgcacagtct
     3181 ggtggcgcag gaacgcagta cggtcgaaga agcctatccc ggcgatatca tcggaatcat
     3241 caatcccggg gtcttcgcca tcggtgatac ggtttcattg accggagggt ttaatttcaa
     3301 acctctgccg cagttccagg ctgaactttt tgcacgtctc aggccaacgg atgtgggcaa
     3361 acgcaaagcg ttcgacaagg gcctgtccca aatggcgcag gagggaacag tacagatcat
     3421 gcgaagtctc aatgatcagg atgccctgat tgcagcggtc ggtcgtctcc agttcgatgt
     3481 gctgcaatac cgactgcgcc atgagtaccg agtggaaacc atcatggaca cactgccgtt
     3541 tacctgtagt gcgtggctgg acggagatcc ctccaccttc aagtcaccgt cggcatcgat
     3601 gatggtgaag gaccaacggg gtcgagtcgt cgtcctcttc ggtgatcagc ttatgaaaac
     3661 catcgcgcgt gaccggaatc ccgaccacga cctgcgtgaa atgggatagg ccggaagccc
     3721 ttagagatag cttgtcccag ccggtgcaga ctgtctctgt tgcaagcatc cacatcgaac
     3781 gcggaatccc atggcttaag agccgcgcct tttggggtcg gttggtgaag gggtgtgtta
     3841 taagaactca agagggaaat cagtggacga acagcttcga aatcagttgg ctcggcatag
     3901 gaccgagttg gctaatgagc ggacgttgct ggcctatatt agaactgcgt taggctttgt
     3961 gattgtcggg gtgccggcca tgtggttgtt ggatcacccg tacatgcaga cgctcggtgc
     4021 gctggcgttg gcggccggcg tgatatgtct tgctctcggc ataagccggt tcgtgacggt
     4081 caaggccatg atcgcacagg aattcggttc ccaagtcgac aaagcggctt aacgtcccgt
     4141 ggaggatttc atgaggctgt cggcttgagg cggaaggaat ccttggtacc ctcgatggcg
     4201 ttcggccaat tctagaacag aaaatggctg cccgtcgatg gtttccggcg acgaaaaaat
     4261 ttgcctgagc ggcccacccg atgctccgac gatcttgccg gcgaacacca cacctttttg
     4321 ctgcagtcga gcgacggcct gttcaatatc ctccacccgg acggccacat ggtgaaagac
     4381 cttgtcaccg aatctatcga cccagccggg aatgatgctg gtcttccctc gtgcatccgg
     4441 ataggcctgg tctacgaata gggcagggta gccggccttg cggtagacct tcgcgtacca
     4501 atcgtcgtac tgcagggttt catcataggc atatcccaac ttcacgaagg gttgagctct
     4561 gcggtcgata tcgagtgtcc gaatggtgac atggtcgatg accggtgaga agcctatacc
     4621 ggtctcgtcc agcatcacct tcaggacccc tgccgcccgg ttacgagcga cataggtcgc
     4681 caccatcttt tccatgagat catcgagttc atgagcttga tccattggtc gcaccccgtt
     4741 caagatccaa acttaatgcc ttgagccagc ggtaattccg tgccccaatt gactgtattg
     4801 gtctgacgac gcatataggc cttccaggag tccgatcctg cttcgcgccc tcctcctgtg
     4861 tctttctccc cgccgaacgc tccgccgatc tcggctccag aagttccaat attgatgttg
     4921 gcaatcccgc aatcgctgcc ggccaccgac aaataccgct cgctgtggcg aacatcattc
     4981 gaaaacattg ccgacgagag accctgcggg acgctgtttt gcatctgaat ggcctcgtct
     5041 atggtttgga aggtcatgac atacaggata ggcgcgaagg tctcgcgttg cacgatgggc
     5101 cagtggtttt gagctcggac gatcgtcggt tccacaaaat acccgggccg attcaacaca
     5161 tgtccgccgc agagaatctc gccgccttct ttcttgattt catcgagcgc gacacgatag
     5221 ccctccacgg ccacgtggtc gatgagcggc cccatgagca tgtctttctc caacggattg
     5281 ccgatctgca cttgggcata ggccttcaca agcctcccga ccaattcctc gtagcgtgat
     5341 tcgtgaacga tcaagcgtct cgtcgttgta catcgttggc cggcagttcc gacggctccg
     5401 aataagatgg cgcgcaaggc catgtccaag tcggcggtct gatcgacgat cacggcgttg
     5461 ttgccgctga gttcgaggag ggttcggccc aatcgctgtc cgactagaga agcgaccctt
     5521 cgaccgaccg gcaccgatcc ggtaaacgag atgagcggta aacgctcatc ttgcaccatc
     5581 tgttccgcaa gagccggctg gtcggcgata cacagcgaaa aaatgcccga atagccctgc
     5641 tgctgcatga cgcgattaca aatctgttgc accgcgacgg cgcagagcgg cgccttcggt
     5701 gacggcttcc agatgacggt atcgccggcg acggcagcca caaacgcatt ccaggcccac
     5761 acggcaacag gaaaattgaa cgcggtgatg acgccgaccg gtcctaatgg atgccattgt
     5821 tcgctcattc tgtgggccgg tcgctcggac tgcatcgtga cgccgtagag catccgtgat
     5881 tgaccgacgg cgaaatcggc catatcgatc atttcttgca cttcaccgtc cccctcggcc
     5941 ttgatcttgc cgacttccag cgagacgagc gtgcccaatt gatccttctt ctcgcgcaag
     6001 gcttggccga tgagccggac catctcgcca cgcttcgggg ccggcaccat ccgccaggtg
     6061 cggaaggcct caaccgattc tttcacgatc cgctggtaat catccgacga acagggatag
     6121 acgccggcga tttgctcgcc agtcgaagga ttgatcgagg ggaggaggga tccatcatta
     6181 cgaccggacc accagccggt tccggtactt ccaccggaat tcacggcttg gataccgagg
     6241 tctcgcagcg cagtgtgtgt ggtgctcatc ggaagcaggc tccaaaatca ttgtccaaga
     6301 aatcttttaa caatacggat tcctgagtga cgaatccatg gtacgccgat ggatgggtca
     6361 acacaagatc catgacgcag caggcgctgg acgcggtcgt cacttggatt gccgaccaga
     6421 gttttccctt gatacattgc ggataaatct tcttcacata gttttcttcg aaaaatccgt
     6481 ccgcgttttg tccggtgacc gacgcataga tcaagacgac atcctggagg gtctgaggaa
     6541 cggcatgttc gaggacacgc ttcagggttt cccggtcttc gttcagttta aggtccttca
     6601 tcaacagatg gattttttca caatggtcgg gataacggag cgtcttatag ttcatcgtcc
     6661 gaacttttct cgcataactg tcggccaagg tacccaatcc acccgaggtg ttgaacgctt
     6721 cataga
//
LOCUS       DATMTI010000063         5573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_341982, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000063 DATMTI010000000
VERSION     DATMTI010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341982"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..815)
                     /locus_tag="VN039_01060"
     CDS             complement(90..815)
                     /locus_tag="VN039_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWV44611.1"
                     /translation="MNTKIGEKPRRSKSLVLCIAIALSVVSTLSAFAETYVSGMVGAT
                     LAPDTVRGRVNDPSFVGLPEGTSISNVQLNSSIMYGAKVGHYFNSMPWLGVELEAFVT
                     TPHRPAQRLTLNAPGVGAIQQDEPGATNRLVVVSPLIMVRYRAGALEPYAGIGPGVFF
                     LHQEQLTASASPSNYSQSNTGYGLNTQVGLRYRLTGHVSMFGEWKYNYGRIDLAGQAD
                     VNHFGITALSQLHHFVFGVGYHF"
     gene            970..1542
                     /locus_tag="VN039_01065"
     CDS             970..1542
                     /locus_tag="VN039_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV44612.1"
                     /translation="MARPAFEQLREALLQAASGEILEIGFGTCLNLRHYPTTITRLFI
                     VDPAILLPARVARRMAAAPYPIQTIHVPAEDLPFPERQFDTVVSTWTLCTIPDPVLAL
                     QEVRRVLKPGGRFLFLEHGRSDDRKIAAWQDRLNPIQNMIGCGCNLNRQIDRLITQSG
                     LTIVHLDRFSMPDVPRLAGEMYRGTATRPD"
     gene            complement(1539..2237)
                     /locus_tag="VN039_01070"
     CDS             complement(1539..2237)
                     /locus_tag="VN039_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWV44613.1"
                     /translation="MMQSSVLSPQHFPDWTQVDDVLLDMDGTLLDRHFDNFFFEEELP
                     RRYAALHALPFEEARERLMTMYRSVEGELAWTDLDYWTKRVGIDVVAMHKELDHMIGF
                     LPGAEEFLRHLRQLGKRVTIVTNAHSTGVSVKVAKTGLDHYVHRIVDAFEVGYLKMRP
                     EYWPNCQRLLGFDPSRSLFMDDDEGCLMAAKAFGVAHLVHSAKSSSQLPPSPLSQFFS
                     IAGFTSLLNGRSPA"
     gene            2319..3701
                     /gene="der"
                     /locus_tag="VN039_01075"
     CDS             2319..3701
                     /gene="der"
                     /locus_tag="VN039_01075"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWV44614.1"
                     /translation="MPSTKSTPIQEPRLPLLPLEGGPPPLVAIIGRPNVGKSTLFNKI
                     LGAKIAIVDDVPGVTRDRNYADATYRNRKFRLVDTGGLDLSASDSMLSLIRRQSELAI
                     AEADILIVLLDGRSGLTPPDHEVVRLLRGVTKPLFYAINKIDTPKSEPLLADFYRLGT
                     DQLHPVSAEHGLGVAELLDAIYPLLPSEEEPDELQQLPRVAMVGRPNVGKSTLVNALL
                     GEERVVVSDVPGTTRDPIDSLVTHHDQRYVFTDTAGIRRRGKVDRGVEGYSVLRSLRA
                     IGRSDIAVLLLDGLEGVTEQDTKIAGAILKQGRACILLINKWDLRVGDPKAREEYERE
                     LRRRFPFLTWAPVLYGSALKSESVRRLFPLLKDVHTMFTKRVPTGALNTWLHKILVSH
                     PLPTRKHKPSAVTKSAFITQVTTKPPVFALFVGHPEDLTPSYLRYLENQLRETYQFTG
                     TPLRLMVRKK"
     gene            complement(3900..4394)
                     /locus_tag="VN039_01080"
     CDS             complement(3900..4394)
                     /locus_tag="VN039_01080"
                     /inference="COORDINATES: protein motif:HMM:NF023276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HWV44615.1"
                     /translation="MILVVDASPLITLARIGSLELLHRLAIQVVVPEAVYHECVTRAH
                     GRPGSIELAQADWIVREQVKNQDKVARLHTRVGRGEAETIVLAQEIHADAVILDDATA
                     RHMAKGEGCRVVGLLGLLLEGKQRGLIPAVQPLLDLMRTTGFFIGEALYMNILRQAGE
                     GSHD"
     gene            complement(4391..4663)
                     /locus_tag="VN039_01085"
     CDS             complement(4391..4663)
                     /locus_tag="VN039_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HWV44616.1"
                     /translation="MGQPLTIDLPDDLLQVLGTADEARQEAKTALVLDLVRRGKVSRA
                     RAAEFLQISLWDFPALLAQYQIPWFDYSSEALREDLKTPLPPRSSK"
     gene            4749..4934
                     /locus_tag="VN039_01090"
     CDS             4749..4934
                     /locus_tag="VN039_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44617.1"
                     /translation="MSPRSNMPGRGIVYDSKWAEINQEKDVLQVEFYPRPDGQPWLIS
                     FVDAVAALDDAKKRLLG"
     gene            complement(4993..>5573)
                     /locus_tag="VN039_01095"
     CDS             complement(4993..>5573)
                     /locus_tag="VN039_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44618.1"
                     /translation="YVVAGEPCALLIEDAASYDRALQQVRTFYNHLRPHQHLYGRTPA
                     EVWADVDVFTPAFKSRRCCGVGNGTGTRQCTVRGNEPDSPAPKECRPRCEPSLRGVTV
                     LTKRKVGGISEQMFIEVVVCCCSALENLVIGSVPPAHSSKTLSICSAGPSRTRGRDIG
                     IQELHGVLFEFRCEPSSLPHVAPPRELHRAPF"
BASE COUNT         1303 a         1594 c         1554 g         1122 t
ORIGIN      
        1 accgtatgga gcaacaatcg gtaagaactc tcggtaagaa accggcggct gatcaaaact
       61 gcaatcagcc gttggatgtt cgattaggcc taaaaatgat acccgacccc gaagacaaaa
      121 tggtgaagct gggagagtgc agtgatgccg aagtgattca catcggcttg cccggccaga
      181 tcaatgcgac catagttgta tttccattca ccgaacatcg agacatgtcc cgtcagccga
      241 tatcgaagac ccacttgggt gttgaggcca tatcccgtgt tcgattgcga gtagttcgac
      301 ggactggccg aggcggtaag ttgctcctga tgaagaaaga aaactcctgg accgattccg
      361 gcatagggct cgagcgctcc ggctcggtac cgcaccatga tgaggggcga taccaccacg
      421 agtcggttgg tcgcacccgg ttcgtcctgc tgaatcgcgc cgacccctgg ggcattcaac
      481 gtcagccgct gggctggacg atggggagtg gtgacgaagg cttcaagttc cactcccagc
      541 cacggcattg agttgaagta atgtccgact ttagcgccat acataatcga gctgttcaac
      601 tggacgttcg aaatggacgt accctcggga aggccgacga aactcggatc gttcactcgc
      661 ccacgtaccg tatccggggc aagggtcgcc ccaaccatgc cggagacata ggtctcggcg
      721 aaagccgaaa gagtcgaaac gacacttaag gcaatcgcga tacagagaac caggctcttg
      781 gaccgtcgcg gtttctctcc gattttcgtg ttcatcgaaa tatctcctag gatggccaat
      841 agaattaggc ttgcagacta tggcctcgat gggcatgctg gctactctgc aaaaggtgag
      901 ggagataccc cgaagggcta aatgggcctg tacgcgactc agatctttcc ccgactgatg
      961 gattgggtta tggctcgacc ggcattcgag caactccgag aagcattgtt gcaggcggct
     1021 tctggggaga tactggagat cggattcgga acgtgcctca accttcgcca ttatccgact
     1081 actatcactc gccttttcat cgtcgatccg gcgattctgc ttccagcacg ggtcgcccgc
     1141 cgcatggctg ccgcgccgta tccgatccaa accatccatg taccggcgga ggacctgccg
     1201 tttcctgagc gacaattcga caccgtcgtc agcacctgga cgctctgcac gattcctgat
     1261 ccggtgctcg cgctgcaaga agtgaggcga gtcctcaaac cgggaggaag atttctcttc
     1321 ttggaacatg gccggagcga tgaccgaaag atcgctgcgt ggcaggaccg gctgaatccg
     1381 atccagaaca tgatcggttg tggatgcaat ctcaaccggc aaatcgatcg gctaatcacc
     1441 caatccggcc taacgatcgt tcacctcgat cgattcagca tgccggatgt gcccagattg
     1501 gcaggtgaga tgtaccgagg cacggcaacc aggcctgatt aggccggtga ccggccattg
     1561 agaagcgatg tgaatccggc aatggagaaa aattgtgaga gcggagacgg cggtaattgt
     1621 gaactcgact tggcgctgtg aaccaggtgg gccaccccga acgctttcgc ggccatcaga
     1681 catccttcat cgtcatccat gaacagagat cgcgacggat cgaaccccaa taagcgctga
     1741 cagttcggcc aatactccgg tcgcattttg agatagccga cctcaaaggc atccacaatc
     1801 cggtggacgt agtggtccag accggtcttg gcgactttga ccgacacacc cgtcgaatgg
     1861 gcgttcgtca cgatggtcac acgcttcccg agctgccgaa gatggcgcaa gaattcctca
     1921 gcgccgggga gaaaaccgat catgtgatcg agttccttat gcatcgccac cacgtcgatg
     1981 ccgacccgct tcgtccagta gtctaggtcc gtccaagcta attcgccttc gaccgatcga
     2041 tacatcgtca tcaggcgttc gcgtgcttcc tcaaacggaa gggcgtgcag cgccgcatac
     2101 cgacgcggca gctcttcctc gaaaaagaag ttgtcgaaat ggcgatcgag caacgtgccg
     2161 tccatatcga gcagcacatc gtcgacctgc gtccagtcgg ggaagtgctg aggactgagg
     2221 actgaggact gcatcatggg caaaagtata tcggaggtcg aacgggaagg cgaggcggtt
     2281 gtctttgaga aaaatcctat gatacggttc gacacattat gccgagcacg aaatccacac
     2341 ccatccaaga accgaggctg ccgttgctcc cattagaagg cggaccaccg cctcttgtgg
     2401 ccatcatcgg ccgacccaac gtaggaaagt cgactctgtt caacaagatc ttaggcgcga
     2461 agatcgccat tgtggacgat gtccccggtg tcacacgcga ccgcaactat gccgatgcca
     2521 cctaccgtaa tcgaaagttt cggctcgtcg acacgggggg gctggatctg tccgcctccg
     2581 acagcatgtt gtcgttgatt cgacgacagt cggaactggc aattgctgaa gcggatatcc
     2641 tcatcgtgct cttggacggt cgctcaggct tgaccccgcc ggatcacgaa gtcgtgcgac
     2701 tattacgggg agtgacgaaa cccctgttct atgcgatcaa caagatcgat acgccgaagt
     2761 ccgaaccctt gctggccgac ttctaccgat taggcactga ccagctccac cctgtgtccg
     2821 ccgagcatgg cctcggtgtc gctgagctcc tggacgccat ttacccgctc cttccgtcgg
     2881 aagaagagcc cgacgagctg caacaactgc ctcgcgtggc catggtggga cgtccgaacg
     2941 tcggcaagtc cactctcgtc aacgcgctgc tgggagagga acgggtcgtc gtcagcgatg
     3001 ttccgggaac gacgcgcgac cccatcgact cactggtcac gcaccacgat caacgctatg
     3061 tcttcaccga cacggcgggc atcagacggc gcggtaaggt tgaccgcggg gtggagggct
     3121 atagcgtcct acgatcactc cgtgccatcg gtcggtccga catcgctgtc ctcctactcg
     3181 atggtctgga aggagtgacg gaacaagaca ccaagatcgc cggagccatt ctgaagcaag
     3241 ggcgtgcctg cattctgttg atcaacaagt gggacttgcg ggtcggagat ccgaaggcgc
     3301 gagaagagta tgaacgcgaa cttcgtcgcc ggtttccttt tttgacctgg gctccggtcc
     3361 tgtatggatc agccctcaaa tcggaatccg tccgccgtct ctttccactc ctcaaggacg
     3421 tgcacaccat gtttactaag cgtgtcccca ccggggcact gaatacctgg ctacataaga
     3481 ttttagtcag ccatccgttg ccgactcgca agcacaagcc gtcggccgtg acgaagtccg
     3541 ccttcatcac gcaggtaacc accaaaccgc cggtctttgc gttgtttgtc ggccatccgg
     3601 aagacctcac accctcgtac ctgcggtact tggagaatca actgcgcgag acataccaat
     3661 tcaccggcac accgctccgc ctcatggtga gaaagaagta agcgctctcc ccttcgaaga
     3721 tctccagacc gtctcgccct cttccaacaa cgccaagtca ttcgtgaaga atcgaccatt
     3781 ctgtacgcaa ttggcctcaa ttaaaaatca atgactctcc acctagttta gtattcgatc
     3841 aaagagaaag ctaaggtaag gtaatgttgc cgagaaaacg gaaataacca aagtgctgct
     3901 cagtcgtgag aaccttcacc ggcttgccgg agaatgttca tatagagtgc ctcaccgata
     3961 aagaatccag ttgtccgcat gagatcgagc agcggttgca ctgccgggat gagcccgcgt
     4021 tgttttcctt ctagtagcag gccgagtaga ccgacaaccc ggcatccttc tcctttagcc
     4081 atgtgtctgg ccgtggcgtc atccaatatc accgcatcgg cgtggatctc ctgcgcgagc
     4141 acgatcgttt ccgcctctcc acgcccgacg cgagtgtgaa gtcgtgcgac cttgtcctgg
     4201 ttcttcacct gttcacggac gatccaatcg gcctgagcca actcgatgct gccgggtctg
     4261 ccatgcgccc gagtaacaca ttcatgatag accgcctcag gcacgacaac ctgtattgct
     4321 aatcgatgca gcaactcgag cgaaccaatg cgggccaatg tgatgagggg actggcgtcg
     4381 acgacgagaa tcactttgag gaacgcggcg gaagcggcgt cttaagatcc tcgcgcaagg
     4441 cctcggagga atagtcaaac caaggaatct gatattgcgc caacaaggcc ggaaaatccc
     4501 agagcgagat ctgaagaaat tctgcggcac gagcccgtga gactttcccc cgtcggacaa
     4561 gatcaagaac cagagcggtc ttcgcttctt ggcgcgcctc atctgctgtg cccagcactt
     4621 gcagcaggtc atccggcaga tcgatggtta atggctgtcc cataggaacg agtgtagcag
     4681 agagccctat ggaatccaat acggagcagg agaactgaag atgccacgct aggaaggcac
     4741 atcacgacat gtcaccaaga tccaacatgc cgggtcgagg aattgtgtat gactccaaat
     4801 gggcagagat caatcaagag aaagatgtgt tgcaagttga gttttacccc cgtccggatg
     4861 gacaaccctg gctgatcagc tttgtcgatg cggttgcagc attggatgac gcgaagaaac
     4921 gattactggg ataggagtag aaatggagaa gaaaagggga cgggctgagc tagcccgggt
     4981 ttgacggaca cctcaaaagg gggcacgatg aagctcccga ggaggtgcga catggggcag
     5041 cgacgacggt tcacaccgga attcaaacaa cacgccgtgc aactcctgaa tgccgatgtc
     5101 ccgtccgcgc gtccgggatg ggccggcact gcaaattgaa agggtttttg atgagtgagc
     5161 gggcggaaca gatcctatga ccagattttc gagcgcggag caacagcaca cgaccacctc
     5221 tataaacatt tgttctgaaa tcccccctac ttttctcttg gtgagcacag ttactccccg
     5281 cagcgacggt tcgcagcgcg gacggcactc tttcggtgca ggggaatccg gttcgttacc
     5341 ccggaccgtg cactgcctgg tcccagtgcc gttcccaacg ccgcagcagc gacggctctt
     5401 gaaagcgggc gtgaagacat ccacgtcggc ccagacctcg gcgggcgtgc gcccgtacag
     5461 atgctgatgt ggccggaggt ggttatagaa cgttcgaacc tgctgcaagg ctcgatcata
     5521 cgacgcggca tcctcgatga gcaacgcgca aggttcccca gcgacgacgt acg
//
LOCUS       DATMTI010000064         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_129952, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000064 DATMTI010000000
VERSION     DATMTI010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129952"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VN039_01100"
     CDS             <1..582
                     /locus_tag="VN039_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV44619.1"
                     /translation="EDLLELSKIESGGISLKKEPLELRSVIDRTLSTIKPIADKNRHR
                     LVTVVDPALPPVAGDEGRLVQVLTNLLDNAIKYTPAGGTITVGAKLTPSVGSTDPPAR
                     AIELSVADTGIGIPEEDRPRVFERFYRVDKARSRELGGTGLGLAIVKHIVEGHRGQVW
                     VEANHPQGSRFVVRLPLSTTARSRHQVNEASRA"
     gene            complement(555..1547)
                     /locus_tag="VN039_01105"
     CDS             complement(555..1547)
                     /locus_tag="VN039_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HWV44620.1"
                     /translation="MTELSGILLLTVVLALLFDFSNGWHDCANAVATVVSTRVLSPIA
                     AVLLAGVLNVAGAFFSTAVAKMIGGGIVFSDAITNTVVAGAMGGAILWNFLTLMLGLP
                     TSSSHALIGGLVGSAVAHGGWAVVQFNGLRKILEAMILSPLLGFGIGFLLMVLVSWVF
                     FRVHRGAATKIFSRLQIVSASFMAFSHGANDAQKVMGVITLALVASGQLTSSEVPTWV
                     IVACALAMGLGTTVGGWRIIRTLGIKMVKLEPVHGFCAETAAASVLLFAAHFGLPVST
                     THTITSSILGVGSTKRLSAVRWGVTNKIFSAWVFTLPGAALLGAGVYALLASFT"
BASE COUNT          380 a          480 c          442 g          274 t
ORIGIN      
        1 gaggatttgc tcgaactgtc gaaaatcgaa tcaggcggga tctcgttaaa gaaagaaccg
       61 cttgaactgc gttccgtcat cgaccgaacg ctgtcaacga tcaaaccgat cgccgataaa
      121 aatcgtcacc ggctcgtcac agtggttgat ccagcgctcc ctccggtagc gggtgatgaa
      181 ggtcggctcg tgcaagtctt gaccaatctg ctcgacaacg ccatcaaata tacgccagca
      241 ggagggacca ttacggtcgg cgccaagctg actccttcgg tcggaagtac tgatccaccg
      301 gccagagcga tcgagctcag cgtcgccgat accggaatcg gtatccccga ggaggaccgg
      361 cctcgtgtct ttgaacggtt ttaccgcgtc gacaaagccc ggtcacgaga gttgggaggg
      421 acgggactcg gcctggcgat cgtgaagcat atcgttgaag ggcatcgagg acaggtatgg
      481 gtcgaagcga atcatcctca agggagtcga ttcgtggtcc gtctgccgct cagcaccact
      541 gcccgcagcc gccatcaggt gaacgaggca agcagggcat agacacccgc tcccaacagc
      601 gcggctccgg gcagtgtaaa tacccaggct gaaaagattt tgttcgtcac tccccatcga
      661 accgcagaca gacgcttggt cgatcccacg cctaagatcg aggaggtgat ggtgtgggtc
      721 gtgctgaccg gaagaccgaa gtgcgcggcg aacagcaaca ccgaagccgc tgccgtctct
      781 gcgcagaatc catgaaccgg ctcaagtttc accattttga tccctagagt ccgaatgatc
      841 cgccacccgc cgacggtggt tcccagcccc atcgccaatg cgcaggccac gatcacccaa
      901 gtcggcactt ccgatgatgt caactgaccg gacgccacga gggcaagcgt aatgacgccc
      961 atcacttttt gcgcgtcgtt cgccccatgg ctgaatgcca tgaagctggc cgaaacgatt
     1021 tggagtcggc tgaatatctt tgtcgcggca ccccgatgaa cccgaaaaaa gacccagctg
     1081 accaggacca tcaggagaaa tccaatcccg aaccccaaga ggggggaaag aatcatggct
     1141 tcgagaatct tgcgcagacc gttgaactga accacagccc agccgccgtg cgccaccgct
     1201 gagccgacga gtccaccgat cagcgcgtgc gacgaactgg tcggcaggcc cagcatcagc
     1261 gtgagaaaat tccagagaat cgcaccgccc atcgctccgg cgaccaccgt attggtgatc
     1321 gcgtcggaga agacgatgcc tccaccgatc attttcgcca cggccgtcga aaagaaggct
     1381 ccggcgacat ttaacacccc ggccaacagc actgcggcga tggggcttag cacacgggtg
     1441 gagacgaccg tcgccacggc attggcgcaa tcatgccacc cattcgaaaa gtcgaacagg
     1501 agcgccaaca ccaccgtcag aagtagtatg ccgctgagtt cagtcatagt ccggttgaaa
     1561 tacttcgctt atccgg
//
LOCUS       DATMTI010000065         4267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_384325, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000065 DATMTI010000000
VERSION     DATMTI010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384325"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..718)
                     /locus_tag="VN039_01110"
     CDS             complement(257..718)
                     /locus_tag="VN039_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV44621.1"
                     /translation="MYVTYIATTPEKTWQALIDTSVMRKYWADPTDDDPAHENVSDWK
                     PGSKWEHRRADGTGTIDIVGKVVEILPPRRLVITWARPKDAEDDSKHSRVAFDIEPHG
                     DRLICLTVTHENLEHDPKMFEGISIGWPKVLSNLKTLLETGHALPRLPSVA"
     gene            complement(894..1697)
                     /locus_tag="VN039_01115"
     CDS             complement(894..1697)
                     /locus_tag="VN039_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWV44622.1"
                     /translation="MMMKHRTGTRQEWLTARLDLLKEEKELTRRSDEVARRRQELPWV
                     RVDKEYRFETNEGNVSLTDLFRGRSQLLVYHFMFGPDYKAGCPSCSSIADGFNGIVVH
                     LENHDVAFTAVSRAPLAKLQAYKRRMGWTFPWASSFGGDFNADFNISFTEEQQREGGI
                     EYNYRREAAARKLPSPGAEISRSTPDGPTTFAAMCGTDAATYILERPGMSAFVLDDGV
                     VHHTYSTYARGLDGLWGMYQWLDRTPLGRNENGLWWHRHDEYDKGKAKS"
     gene            2017..3240
                     /locus_tag="VN039_01120"
     CDS             2017..3240
                     /locus_tag="VN039_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44623.1"
                     /translation="MDQLITVAARALAAGDPLGALNRVALRDDAPALALRGIAMAQLG
                     DLVRAKALLQRATRAFGSKEAVARARCHVAQAEIALVSRDLGWAAKALSTAGATLEAH
                     GDRVNAAHARYLEVRRLLLIGRLDEAERRLADLDPALLPPTLRTAHELVVAGIAIRRL
                     RTKAARAALVRAKRTAHAARIPTLTAEVESACFSLNTPAARLIASGGERVLVLKEVEA
                     LLASKTFVVDACRHTVHHANKVVSLAKRPVLFALARALGEAWPRDVPRDRLVARAFES
                     KFVNESYRARLRVEIGRLRRMLRTLANVNATKQGFALAPRGARAVAVLAPPVDGEHAR
                     VLAFLADGESWSSSALALALRASQRTVQRALDSLAAAGKVQWFGHGRARRWMALPLPG
                     FATTLLLPAPFPESL"
     gene            3248..3913
                     /locus_tag="VN039_01125"
     CDS             3248..3913
                     /locus_tag="VN039_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWV44624.1"
                     /translation="MNQSPAEIVHEYGPFSGADHVHGVTYDGQQVWFAAGDKLIAINP
                     ASGKTVRSIDVAANAGTAFDGKHLFQIAEGRIQKIDPKTGQVLSTIPAPSGDDNSGLA
                     WAEGMLWVGQYEGRKIYQVDPQTGAILRTIESNRFVTGITWVDGELWHGTWEGDKSDV
                     RRIDPRTGEVLEKIEMPTGTGVSGLESDGGDLFFCGAGRSGKVRTVRRPKRSSATRSN
                     PKT"
     gene            complement(4031..>4267)
                     /locus_tag="VN039_01130"
     CDS             complement(4031..>4267)
                     /locus_tag="VN039_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44625.1"
                     /translation="RAQSGQDAPQFDGRSEIQEIKILGDWAFMWSKLTVVVTPPGGAQ
                     SLTRAGHTLTIFKKQNGKWVLARDANMLVPVSNA"
BASE COUNT          881 a         1221 c         1203 g          962 t
ORIGIN      
        1 ggcgatcatc tttcagaatc gctgcaagaa gggattcgaa tgcttcgctc acatgaccct
       61 cgaggtttcg agaaaagtca ctcatcatcg gattgcgcca tatcgaagca caaggcccac
      121 tatactcgaa tgacgctatc cagcactccc agatctcatg gccgggggtt caggtaccgg
      181 ccgcttcttg cgagcaactc cgataggtag ccatctacgc ttcacacaac atcaccttca
      241 tacctctccc gtctctttaa gctactgaag gcaagcgtgg gagagcatga ccagtttcga
      301 gcaacgtctt gagattcgaa agcactttcg gccaaccgat cgatatgcct tcgaacatct
      361 tggggtcgtg ctcgaggttt tcatgcgtga ccgtcaagca aatgagcctg tcgccgtgtg
      421 gctcgatatc gaaagcgaca cgcgaatgtt tcgaatcgtc ctcggcgtcc ttgggtctcg
      481 cccacgtgat aaccaagcgt cgtggcggga ggatctcgac gaccttcccg acaatgtcga
      541 ttgtgcccgt accatcggcg cgtcggtgct cccatttaga accgggcttc caatcggaca
      601 cattctcatg ggcagggtcg tcgtcggttg gatcagccca atacttacgc atgacactcg
      661 tgtcgatcaa tgcctgccac gtcttctccg gcgtcgttgc aatataggtt acgtacacga
      721 agctcggctt aggtgcttag ctccaatcgt cgatcttgac gtcgttgggg tatggagcac
      781 ccttgccggt ttccaggagg gactttaaac tcatcagaaa aattccccac ttggtgctgc
      841 aatgatgcat aaattcgatg ggcttctttg gaccagttct cattgcgcct tggtcacgac
      901 tttgccttgc ctttgtcata ctcgtcgtgg cggtgccacc agaggccgtt ctcgttgcgt
      961 cccaggggag tgcgatcgag ccactggtac atgccccaga ggccgtccag tccgcgagca
     1021 taggtggaat aagtgtgatg caccacgccg tcatcgagca caaacgcgct catgccgggc
     1081 cgctcgagga tatacgtcgc tgcatcggtt ccgcacatgg cagcgaatgt cgtggggccg
     1141 tcgggcgtcg agcgcgagat ctctgcgcca ggcgagggca gcttccgtgc cgccgcctcg
     1201 cgccggtagt tgtattcgat gcctccttcg cgctgttgct cctccgtgaa cgagatgttg
     1261 aagtcggcgt tgaagtctcc gccgaacgag gacgcccagg ggaacgtcca ccccatccgc
     1321 cgcttgtacg cctgcagttt ggcaagcggt gccctggaca ccgccgtgaa cgcaacgtca
     1381 tggttctcaa ggtggacgac gatgccgttg aacccgtccg cgatggacga gcaggacggg
     1441 cacccggctt tgtagtcggg tccgaacatg aagtgataga cgaggagctg cgaacgccct
     1501 cgaaagaggt ctgtcagcga gacattcccc tcgttggtct cgaatcggta ttccttgtcg
     1561 acccgcaccc acggcaattc ctgtcgccgc cgcgccactt cgtcgctgcg ccgcgtgagc
     1621 tccttctctt ccttgagcag atcgagcctc gctgtcaacc attcttgtct tgttccggtc
     1681 ctatgcttca tcatcatttt tctccttcct ttctgtgtta tttgttgtta cttcttgttt
     1741 aatattcact accttcacca tcttcatatc ttcttctcgt gcatgacgtt atcctctctt
     1801 ttcttgctgc ggaacttgct tatgaccaca gcagcgatac cgccggttga ggttgaaacg
     1861 gcgccggcgg caatccaggt tacggctgag atacacatcg gacacatggc taccatccct
     1921 tgtcgctggt catccgatag atttcatcgt tcgtcatgag ctagatttag caccgagtac
     1981 ccgaatggtg ggagtgacaa gtgtgacggg attccggtgg accagctgat cacggttgcg
     2041 gcgcgcgcat tggccgcggg tgatcccctc ggcgcattaa atcgcgtcgc tctgcgtgac
     2101 gacgcccctg cgctcgcgct tcgaggcatt gccatggcgc agctcggcga tcttgtccgg
     2161 gcaaaagctc ttctgcaacg tgctacgcgt gcttttggtt cgaaggaagc ggtggctcgc
     2221 gctagatgtc acgtcgcgca agccgagatc gcgcttgtct cacgcgacct tggctgggct
     2281 gcgaaggcac ttagcactgc gggagcgacc cttgaagcgc atggcgaccg ggtcaacgca
     2341 gcgcacgccc gttatcttga ggttcggcgc cttctcctga tcgggcgact cgacgaagcc
     2401 gaacgtagac tcgctgacct cgaccctgcg cttctcccgc cgacattgag gactgcccat
     2461 gagctggtgg tcgcaggaat cgcgattcga cgcctcagga cgaaggcggc acgtgccgcg
     2521 ctcgttcggg ccaagcgtac agcgcatgcc gcacgtatcc ctacgctcac agcggaggta
     2581 gaaagcgcat gtttttccct gaatacacct gctgcacgct tgattgcaag cggtggggaa
     2641 cgggttcttg tcctcaaaga ggtcgaagca ttgctggcat cgaaaacatt cgtcgtagac
     2701 gcatgccgtc ataccgtgca ccatgcaaat aaggtagtct cactcgcaaa gcgtccagtg
     2761 ttgttcgcgc tcgcacgtgc gttgggcgaa gcatggcctc gagacgtacc gagggacagg
     2821 ctcgttgcac gagcgttcga atcaaagttt gtcaatgaat cgtatcgtgc cagattgcgt
     2881 gtcgaaatcg gacgacttcg caggatgctt cggacactag ccaacgtgaa cgcaacgaaa
     2941 caagggttcg ctctggcgcc gcgtggcgca agagcagtgg ccgtgctggc accgcctgtc
     3001 gatggggaac atgcgcgggt acttgcattc ctcgctgatg gagaatcgtg gtcgagttca
     3061 gctttggcgc ttgcgttaag ggccagccag cgcaccgtgc agcgggcact cgactcgctt
     3121 gcggcggccg gcaaggtgca atggtttggt catgggcgag ctcgccgttg gatggccctg
     3181 cccctgcctg gattcgcgac aactttatta ctcccagccc catttcctga gtcactatga
     3241 gggaagcatg aatcaatcac cagccgaaat cgtccatgag tatggtccct tttccggggc
     3301 agaccacgtg cacggagtca cctatgacgg tcagcaggtt tggttcgccg ccggggataa
     3361 gctgatcgcc atcaatcctg cgagtgggaa aacagtgcgc tcgattgatg tcgcagcgaa
     3421 tgcaggaaca gccttcgacg gcaagcatct atttcaaatc gccgagggcc gtatccagaa
     3481 gatcgaccca aagaccggcc aagtactctc cacaatccca gcgccaagcg gcgacgataa
     3541 ctcgggactc gcatgggcgg aagggatgct ttgggtggga caatatgagg gccgaaagat
     3601 ctatcaagtg gatccccaaa caggcgcgat tcttcgcacc atcgaatcca atcgcttcgt
     3661 caccggaatc acctgggtcg atggagaact ctggcacggt acgtgggaag gtgacaagag
     3721 cgacgtacga cgaatcgatc ctcgaacggg agaagtttta gagaagatcg agatgccgac
     3781 tggaacgggt gtgtcgggac tcgagtccga tggcggcgat ttgttctttt gcggtgcagg
     3841 aaggagcggc aaggtaagga ccgtccgccg tcccaaacgg agctccgcga cacgtagcaa
     3901 ccctaagact tagacaaaga ctccacgagc aaataattga agcggggcgc ttacgctcat
     3961 tgctgtcaat aaccactagg tcgaaattca gcacctcttc ccgaactcca ataaagtgct
     4021 ttgttgaatg tcatgcgttt gacacaggaa ccaacatgtt ggcatcgcgg gccagtaccc
     4081 atttcccatt ttgcttcttg aagatggtca atgtatgacc tgcacgcgtc aaggattgag
     4141 cgccgccagg tggagtaaca acgacggtga gtttcgacca cataaaagcc cagtctccta
     4201 gaattttgat ctcttggatc tcgcttctgc cgtcaaattg cggggcatct tgtccagatt
     4261 gagcacg
//
LOCUS       DATMTI010000066         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_554610, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000066 DATMTI010000000
VERSION     DATMTI010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554610"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VN039_01135"
     CDS             complement(<1..735)
                     /locus_tag="VN039_01135"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWV44626.1"
                     /translation="MYNRSESDLLRMLADRTGIVADYYDIAGTHHVTTDETRRAILAA
                     MNLRVANRVELMAELEAWDNRWWLRGCEPVHVLRLGRDAGTWSFYGPCESSDDSVFQI
                     LWSLYAENGETQCERAEGPGLRVEETRTIQGRRFVRVALAFPQDLPLGYYTVKAHAKK
                     GGTNTESTFHLIVAPERCYIPDELQQGVRWWGVALQLYSLRSHHNWGIGDFRDLGAVI
                     EWAGKRLGAAVVGLNPLHALKNAKPYH"
     gene            913..1230
                     /locus_tag="VN039_01140"
     CDS             913..1230
                     /locus_tag="VN039_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HWV44627.1"
                     /translation="MRCSSDLRRRVVSFVRGGGSKAEAARRFKVGEASVYRWLKPGGL
                     THKRPGPKTAHKLDWEALRRHVTTHDDLTQAEAARHFGVSRHCIWNALHKLGWTRKKN
                     DRL"
     gene            1244..1741
                     /locus_tag="VN039_01145"
     CDS             1244..1741
                     /locus_tag="VN039_01145"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWV44628.1"
                     /translation="MQRRKFLRLRERYIRRGLQSVHVDECGFTPFVTRRYGYAPKGQR
                     VYGLTSGHRRPRTSLIAARIGPDFAEPFLFDGTCDAAVFNSWLRARLCPRLTREHLVI
                     MDNAAFHKSPETAQLIRHTGATLLFLPPYSPDLNPIEHDFAALKKHWEYHETASIAQI
                     VKAYQ"
BASE COUNT          393 a          516 c          487 g          362 t
ORIGIN      
        1 atgatagggc ttggcattct tgagagcatg gaggggattc agcccgacca ctgcagcgcc
       61 cagacgtttt cccgcccatt cgatgacggc gcccaggtct cgaaaatcgc cgattcccca
      121 attgtgatgg ctccgcaatg aatagagctg caaggccacg ccccaccatc gaacaccctg
      181 ctgaagttca tcgggaatgt aacagcgctc cggcgcaacg atgaggtgga atgtggattc
      241 cgtattcgtg cctccctttt ttgcatgggc tttgactgtg taatagccaa gcggcaggtc
      301 ttggggaaat gccaaggcga ctcgaacaaa cctgcgtccc tgaatcgtgc gggtctcttc
      361 gactctgaga ccaggcccct ccgctcgctc acattgcgtt tctccgttct ccgcatagag
      421 tgaccacagg atctgaaaaa ccgaatcgtc cgagctctca cagggtccat agaacgacca
      481 ggtacctgcg tcccggccca atcgaagtac atggacaggt tcgcagcctc gtagccacca
      541 gcgattatcc cacgcctcta gttccgccat gagttccaca cgattcgcaa cccgcagatt
      601 catggcggcc aagatggccc gccgcgtctc gtccgtcgtc acatggtgcg ttccggcgat
      661 gtcgtagtag tcggcaacaa tccctgtccg atcggccagc atgcgcaaga gatcactttc
      721 agatcgatta tacatactgg gagtctgagt gaacggctag atcaagtcaa ggtaatgcag
      781 actctagacc atttgattga atcggctgga aaactcacca gataggttgt cgccaaaccg
      841 gggatcccag aggttatagc taagcccagg attactgata gacagagggg ttgtactctg
      901 gaagaatagg gcatgaggtg ttcatcggat ttgcgtaggc gggtagtaag ttttgtgcga
      961 ggcggcggaa gcaaggcgga agcggcccgg aggttcaagg tgggcgaggc gagcgtgtat
     1021 cggtggctca aacccggtgg gctgacacac aagcgaccgg ggcccaaaac cgctcacaaa
     1081 ctggattggg aggccttgcg gcgtcatgtc accacgcatg acgacctgac acaggcggaa
     1141 gcggctcggc atttcggggt ctcacgccat tgcatctgga acgcactcca caagctggga
     1201 tggacccgga aaaaaaacga ccggctataa agagcgcagc ccgttgcaac gaagaaagtt
     1261 tctgcgtctt cgtgagcggt acatacgtcg cggcctgcag tctgttcatg tcgatgagtg
     1321 cggcttcacg ccgttcgtga ctcgccgcta tgggtatgcg cccaagggcc agcgcgtgta
     1381 tggcctgacg tccggacata gaagaccgcg cacctcactg atcgccgccc gcatcgggcc
     1441 ggattttgct gaacccttcc tcttcgacgg cacctgcgat gcagccgtgt tcaactcctg
     1501 gctccgggca aggctatgcc cgcgtctcac ccgtgagcac ctggtcatca tggacaacgc
     1561 ggcctttcac aaatcgcccg aaacggcgca gcttatccgg cacaccggcg ccacactcct
     1621 ctttttgcca ccctattcac ccgacctgaa tcctatcgag cacgacttcg ccgcgctcaa
     1681 gaaacactgg gaatatcatg aaactgcttc cattgcccag atcgtgaagg cctatcaatg
     1741 attatcggct tggctata
//
LOCUS       DATMTI010000067         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_681603, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000067 DATMTI010000000
VERSION     DATMTI010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681603"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VN039_01150"
     CDS             <1..306
                     /locus_tag="VN039_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HWV44629.1"
                     /translation="VTEASRQLKLLARDLECPVIALSQLNRNSESRMDKRPALSDLRE
                     SGAIEQDADLVVLLHRDMEPRPEQVNEFDMLVAKNRHGQTRALSMDWHGEFMSVLDR"
     gene            318..671
                     /locus_tag="VN039_01155"
     CDS             318..671
                     /locus_tag="VN039_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44630.1"
                     /translation="MAFVNVSGTVTRTFYQGKGAEVTESFEVKGKEVKKRWTAWFDTE
                     HGLSEGDVVEVSGLHGDEVSEWTDKDDQVRHTVKRSLNKAKLKGEPQGSHVPVQEQEA
                     PAVSGWDAAPDESPF"
     gene            671..841
                     /locus_tag="VN039_01160"
     CDS             671..841
                     /locus_tag="VN039_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44631.1"
                     /translation="MSEYEGPTEIGYANWPGRGTTYHFPVAGKQVEIYVTEKQKRVRL
                     FVDAVEWKPADV"
     gene            990..1373
                     /locus_tag="VN039_01165"
     CDS             990..1373
                     /locus_tag="VN039_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44632.1"
                     /translation="MGANDDREMLVAAKAECVRLERENATLRADLDARFEHSDHQSAK
                     YYRRMRDAERRGGELREQLAEARSEIERRPEPAFRDALIADRNKAESALAEGGNDILA
                     VDPGFAYATEEQIEADKKWIKGQRP"
     gene            1370..>1681
                     /locus_tag="VN039_01170"
     CDS             1370..>1681
                     /locus_tag="VN039_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44633.1"
                     /translation="MSEKPGRFDPAFRADLIEFARDRIQSVWDDVPEGVTAQVLAENI
                     VHAQEFLWLSLQNPLPAHDEQVRADTLRESREQAWRDGYYTGKRDYAGSIMGGMPMST
                     PN"
BASE COUNT          358 a          393 c          593 g          337 t
ORIGIN      
        1 gttacggaag catcccggca actgaaactg ttggcccgcg atcttgagtg cccggttatt
       61 gccctgtccc agttgaatcg taatagcgag tcgcggatgg ataagaggcc ggcgctcagc
      121 gatttgcggg agtcgggtgc gattgagcag gacgccgatt tggttgtttt gttgcatcgg
      181 gatatggagc cgcgcccgga gcaggtgaac gagtttgaca tgttggtggc gaagaacagg
      241 cacgggcaga ctcgcgcgtt gtctatggat tggcatggtg agttcatgtc tgtgttggat
      301 cgataaggga gaaacagatg gcttttgtga atgtgagtgg gacggttacc cggacctttt
      361 atcagggcaa gggggctgag gtcaccgaaa gttttgaggt caagggtaag gaagtcaaga
      421 agcgttggac ggcctggttc gacacggagc acggcctgtc tgagggtgat gtggtggagg
      481 tttccgggtt gcatggtgat gaggtttcgg agtggacgga taaggatgat caggttcgtc
      541 acactgttaa gcgttcgttg aataaggcga agttgaaggg tgaacctcag gggtcgcatg
      601 ttccggttca ggagcaggaa gcacccgccg tgagtggttg ggatgctgcc ccggacgaaa
      661 gtcctttctg atgtccgagt atgaaggtcc gactgagatc ggttacgcga attggcccgg
      721 acgcggcacg acctaccact ttccggttgc cggtaagcag gttgagattt acgtgaccga
      781 gaaacagaag cgcgtccggc tgttcgttga tgccgtggaa tggaaacccg ctgatgtcta
      841 atcacagggt gtgtcgaggg tgacggggca gacaacgcct gccccgtccg ggcgttcgac
      901 tgttccgcgt gagcatgtgt gcggcaattg ccgtaacccg tggtcgaata cggggcggca
      961 ggactgcccc aaagcgaagg aggtgtctga tgggcgcgaa cgatgaccgg gaaatgttgg
     1021 ttgcggcgaa ggctgagtgt gtgcggttgg aacgggagaa cgcgacactg cgggcggatc
     1081 ttgatgcacg gtttgagcat tcggatcacc agtctgcgaa gtattaccgg cgcatgcgtg
     1141 acgcggaacg acgcggaggc gaactgcgcg agcaactggc cgaggcacgg tctgagattg
     1201 agcgacgccc tgagccagca ttccgggatg cactgatcgc ggaccggaac aaggccgagt
     1261 ccgcattggc cgagggaggc aacgacatcc ttgctgttga tcccgggttc gcatacgcaa
     1321 ccgaggagca gatcgaggcg gacaagaaat ggatcaaggg gcaacgacca tgagtgagaa
     1381 gccaggccga tttgatccag cgttccgtgc tgacttgatc gagtttgcac gtgatcgcat
     1441 ccagtcggtg tgggatgacg tgccggaagg tgtgaccgcg caagtgctgg cggagaacat
     1501 tgtgcacgcg caggagttcc tgtggctctc gctgcaaaac cccctgcccg cccatgacga
     1561 gcaggtacgg gcagacaccc tacgagagtc acgtgagcaa gcgtggcgcg atggctacta
     1621 caccggtaag cgcgactacg ccggaagcat catgggcggg atgccgatgt ccacgccgaa
     1681 t
//
LOCUS       DATMTI010000068         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_639272, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000068 DATMTI010000000
VERSION     DATMTI010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639272"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..743
                     /locus_tag="VN039_01175"
     CDS             519..743
                     /locus_tag="VN039_01175"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HWV44634.1"
                     /translation="MRRTVKKTSNVKAKPSSGPSKARKPIELPGGIWERISRKAYELW
                     EQRGRQEGSALRDWLDAEEIVMEEIHEARE"
     gene            727..2085
                     /locus_tag="VN039_01180"
     CDS             727..2085
                     /locus_tag="VN039_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44635.1"
                     /translation="MKHASDQAAGFWTPRPSSLDGVLSRLDNLSRKPELMLQRELAHA
                     RALKPYLEGGAGRLVAPLEQEVELASLYLFCDYYPDDGQLTLIEQLRDVITEHIAEEE
                     RQWLDPLKHSYLDILKPTSPPTPGQDLVLQSLADGTRLILPGGDFAEEFATDRPLLTR
                     VIHDPSTQESDRAAWAGCGVAMTQADAEVLLHITSEWRREMEVTTGSFALGEWKEFTK
                     QFGYMILWAFAQRRMAALVDAVVHIGYRNPNGQPYLYAVALYDHHEHRFFTDVLSEMN
                     EFHADTQSAPEDRPSVRRWVQREEGKLVARLTLTAFQLFVECDSPQRLDHIKHRLAAS
                     LGFSLHFRGETLVPPARQLSVAELMSDEPPTVVVTREEDRILLNQFLEKAYLEWSDQP
                     HLALTGRTPRYAAATATLRSKVGDLIDDMERHDPGRQRFGKPAFGYNRLRAHVGLEEL
                     PE"
     gene            2190..>3349
                     /gene="pfp"
                     /locus_tag="VN039_01185"
     CDS             2190..>3349
                     /gene="pfp"
                     /locus_tag="VN039_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HWV44636.1"
                     /translation="MTIERQTVGILVGGGPAPGINSVISAATIHSILGGSDVLGIIDG
                     FKWLMEGGTGQVRPLSIEEVSRIHFQGGSYLGTSRANPTRTPESLDNVLFALSSLGVT
                     RLMTIGGDDTAFSAMKLEERAGGRLQVIHVPKTIDNDLDLPHGIPTFGFQTARHVGAE
                     ILKNLMVDARTTTRWYLVVTMGRKTGHLALGIGKAAGATLTIIPEEFRERPVTLQRIV
                     DLLIGAVIKRLDHGRADGVAVLSEGLIEILDPRDLGGLEHVERDDHGHLRLNEVDVGD
                     VLRRELTKQLHQLGLSTSLVAKNVGYELRCADPIPYDIEYTRDLGYCAAQYLLDGGTS
                     AMVSIQNGRFSPIPFKQMVDSSTGRTKIRIVDIDSQSYQIARQYMIRLTEAD"
BASE COUNT          750 a          923 c          984 g          692 t
ORIGIN      
        1 taagtgagat aaaacgtgag atgtgaaagg aattggaagc agttgtacgc agaatctgtg
       61 ttacgccgca cagcttgctt cttccctctt atctcgtatg gtgccttctg ctacaggacg
      121 cgaacgaccg gtgtagcaga gctccccttc aagagctcaa accgctgatg gcgtatggca
      181 gaagcaagaa ggtctgatca gcgccgattt ctttctcgtg tcacaagcct acgtgtcata
      241 agctcctggc tcgcacagtg gcaccaccct tgctcggctc agaggacagg agggctggtc
      301 atgccgcaag agataggtct cctgaccatt ctcttttgtc atggggactc atggccgtcg
      361 cggcttgtct catgtattat tcggcagtgt cgcggagtcc gtctttcgga gatcctcata
      421 ccctgtctga cggtccgcag tccgaagttt cgcccaggcc atcgtcgtgg gctttcaagt
      481 caactcattc cagcaagtca ttaactgagg agcaaaccat gcgaaggacc gtgaagaaaa
      541 ccagcaacgt gaaagcaaaa ccctcctctg gaccaagcaa ggccaggaag cccatcgaat
      601 tgccgggtgg gatatgggaa cggatctcgc ggaaagcgta tgagctctgg gaacagcgcg
      661 gtcgtcaaga aggcagcgcg cttcgagatt ggctcgacgc ggaagaaatc gtcatggaag
      721 aaatccatga agcacgcgag tgaccaagcg gcaggattct ggactccgcg gccttcctct
      781 ttggacggag tgctctcacg tctggacaac ctttcgcgta aacccgaact catgttgcaa
      841 cgtgaactgg cacacgcacg agcactcaag ccgtatttgg aagggggcgc aggacggctt
      901 gttgcgccgc ttgagcaaga agtcgaactg gcaagcctct atctgttttg cgactattat
      961 ccggatgacg gacagctgac gctgatcgaa cagctgcggg atgtgatcac cgaacacatt
     1021 gccgaggagg agcggcagtg gctcgatccc ttgaaacatt cgtatctcga tatactgaaa
     1081 ccgacctcgc cgccgacacc cggccaagat cttgtgctgc aatcacttgc tgatgggacg
     1141 aggctgatcc taccaggcgg agacttcgcc gaggagtttg cgacggaccg tccgttgctc
     1201 acccgagtca tccatgaccc aagcacccag gagtccgaca gggccgcatg ggccggttgc
     1261 ggagtcgcca tgacgcaagc cgatgcggaa gtcttgctcc acatcacgtc ggaatggcgt
     1321 cgagagatgg aggtgaccac cgggtctttt gcgctgggag aatggaagga gtttaccaag
     1381 caattcggct acatgattct ctgggcattt gcgcaacgac gcatggctgc cttggtggac
     1441 gccgtcgtcc atatcggata tcgcaatcct aacggccagc cctatctgta tgcggtcgct
     1501 ctgtacgacc accatgaaca tcgattcttc accgacgtcc tgtccgagat gaacgaattt
     1561 catgcagaca cgcagagcgc gccggaggat cggccctcgg ttcggcgatg ggtacaacgg
     1621 gaggagggaa agctcgtcgc ccgactcacg ctcaccgcgt ttcaactctt cgtggaatgc
     1681 gacagcccgc aacggctgga ccacatcaag catcggctcg cggcctcgtt gggattttca
     1741 ctgcatttcc gaggcgagac gttggtgccg ccggcccggc aactatcggt ggccgaactc
     1801 atgtccgatg aacctccgac ggtggtcgta acacgcgaag aagaccgcat actgctcaac
     1861 cagtttttgg agaaagcgta cttggagtgg tcggatcagc ctcatctcgc gctcaccgga
     1921 cggacgcctc ggtatgcggc ggcgactgcg acgttgcgga gcaaggtcgg tgatctaatc
     1981 gatgacatgg agcggcacga tccgggccgt cagcggtttg ggaagcctgc cttcggttat
     2041 aaccgtcttc gcgcccatgt cggcttggaa gaactgccgg agtaattcga gcctcaccgc
     2101 ttgcctcttg acgagatgga gtatacaacc atgccgaaga tgatgaagaa atcgaatggc
     2161 cgatgggaga taacgagaaa agaaagccga tgacaataga gcgtcagacc gtcgggattc
     2221 tcgtcggtgg cgggccggct cccggaatca atagtgtgat cagcgccgcc acgatccaca
     2281 gtattctcgg cggctcggac gtcctgggga tcatcgatgg gttcaaatgg ctcatggaag
     2341 gaggcacggg acaagtacga ccgctctcga tcgaggaggt gagccgaatc cattttcagg
     2401 gagggtccta cctgggcaca tcacgtgcga acccaaccag aaccccagag tctctcgaca
     2461 acgtactgtt cgctctgtcg agtctcggcg tcacgcgctt gatgacgatc ggcggagacg
     2521 ataccgcgtt ttcagcgatg aagctggagg aacgagccgg cggccgtctt caagtcatcc
     2581 acgttcccaa gaccatcgac aacgatctcg accttccgca tggcattccg acgttcgggt
     2641 ttcaaacggc tcgtcacgtc ggtgccgaaa tcctgaagaa tcttatggtg gatgcccgta
     2701 ccaccacacg ttggtatttg gtcgtgacca tggggcgcaa gaccggccat ctcgccttgg
     2761 gtattggaaa ggcagccggt gccacgctga cgatcattcc ggaggagttt cgcgaacgac
     2821 cggtcacact gcaacggatt gtcgacctgt tgatcggggc cgtgattaaa cggttggatc
     2881 atggtcgggc cgatggggtt gcggtgctgt ccgaaggact gatcgagatt ctcgatccgc
     2941 gtgatcttgg tggattggag catgttgagc gagacgacca cggccatttg cgattaaacg
     3001 aggtggatgt cggcgatgtg ttgcggcgcg aactgacgaa gcagcttcac cagttgggac
     3061 tttccaccag cctggtggcg aagaacgtgg ggtatgagct ccgctgcgcc gatccaatcc
     3121 catacgatat cgaatacacg cgagacctcg gctattgcgc cgctcaatat ttactcgacg
     3181 gcgggacctc ggccatggtc tccattcaaa acggacggtt cagcccgatt ccgttcaagc
     3241 agatggtgga ttcctccacc ggacggacca agataagaat agtggatatc gattcccagt
     3301 cctaccaaat cgcccggcaa tacatgattc ggctcacgga agccgactt
//
LOCUS       DATMTI010000069         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_427079, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000069 DATMTI010000000
VERSION     DATMTI010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427079"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VN039_01190"
     CDS             complement(<1..660)
                     /locus_tag="VN039_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44637.1"
                     /translation="MPLYGTGQGFQAYAGIRAEDAKTNLVNIQAKVAQTELQQKQQMM
                     QLISRQGLAPVGPGGTPVPASPDQLLDRTRSVAGIALATGNFKEGLEAARTAEQMAKD
                     KSMQAAARSRKEYTDFQEQQKELQEIDGLLAGVHDQASFQAAKMTYMAEHPGIQVPPQ
                     FAYYNPQMVEAIRVGTTQGLAKLRQEANDKAKAQTAIDRADAISSRRFRDNLAAKREQ
                     RE"
     gene            complement(662..1279)
                     /locus_tag="VN039_01195"
     CDS             complement(662..1279)
                     /locus_tag="VN039_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44638.1"
                     /translation="MSSLGSVASPDYSISSPGGLSGGDTGIGFTPTASGSDSFSLTGD
                     GSGSSSLLDKLKSYLGELGSAGKTIGKVGGGLNILSGLYGLTQANKVAAAADPFAPYR
                     SQYATQLSALEANPSSITSLPGYTAGLQAVQRAQAAQGYTGSGNAMNALAQYGQGFYN
                     QQVQQLAALAGAGATPGAGAVPAANITSSSLGTIGLGAAALLKGA"
BASE COUNT          409 a          505 c          440 g          399 t
ORIGIN      
        1 ttcccgctgc tctcgcttgg cagctagatt atctctaaag cgccgggaag aaatagcatc
       61 ggccctatct atcgctgttt gcgcctttgc cttgtcatta gcttcttgac gaagcttggc
      121 caatccttgc gttgttccca ctcgaatcgc ctcaaccatc tgtgggttat aataggcaaa
      181 ttggggcggg acttgaatcc caggatgctc cgccatgtaa gtcatcttgg cagcttgaaa
      241 agaagcttga tcatggacgc cagctaaaag accatcgatc tcttgcagct ctttctgttg
      301 ttcttggaag tccgtatatt ccttccttga acgagcagct gcctgcatgg acttatcttt
      361 ggccatctgt tcggcagttc ttgcggcttc cagcccttct ttgaagtttc cggtagccag
      421 agcaatccct gcaacactcc tagttcggtc aaggagttgg tcaggagagg caggcacagg
      481 agttcccccc ggaccaacag gcgcaagtcc ttgcctagaa ataagctgca tcatctgttg
      541 cttttgctgc aattcagtct gagcaacctt cgcttggata ttaacaagat tggtcttcgc
      601 atcctcagcc cgaatgcccg cgtaggcttg aaacccctga ccagttccgt aaagaggcat
      661 gttaggctcc tttcaacaag gctgctgcac caagacctat ggtaccgaga gaggaactag
      721 taatatttgc cgcgggcaca gcaccagctc ccggcgtagc tcctgcccct gcaagagctg
      781 caagctgttg gacttgctgg ttgtagaagc cttgaccata ctgggccaac gcattcattg
      841 cattaccaga gcctgtgtat ccttgggccg cttgcgctcg ttgaacggct tggagacctg
      901 ccgtataccc aggaagacta gttatcgacg aaggattggc ttcgagagca gaaagctggg
      961 ttgcatattg cgagcgataa ggggcaaatg gatcggcagc tgccgcgact ttatttgcct
     1021 gagtaagtcc atataagccg gataagatgt taagcccgcc gcctactttc ccgattgtct
     1081 tgccggcaga tccaagttcc cccaaataag atttaagttt gtccagaagg gaactcgaac
     1141 ccgaaccatc tccagtcagg gagaatgaat ctgatcctga ggcagtggga gtaaacccga
     1201 ttccagtatc tccgcctgat aagcctccag gagaggagat ggaataatca ggagaagcta
     1261 ccgagcctaa gctagacata tctaactgag atgctgttcc aggagttgcg gcaggtgaca
     1321 atgaatctgt ggtgccaaag gtagacggag cagtcaatcc ttgaccacta gcaggtgctc
     1381 cacctgcccc gccgagggaa gtatctagca gacctgcact tccgccggtc cctaaagtac
     1441 tcattgttcc aaggtcggga gcaaggtctg ctcccgccgg agccgtcaat cccaagccag
     1501 aaggcgctgt tagggaaagt ccctgcgcac cgcccatatc aacgccgccg gcagtggcac
     1561 tcccagcccc cgagcctaag ccgcctagat agctagctcc tgctccacca gcgcccccga
     1621 gtgcagctcc tcgcagcata ccagaagtac caccaccgta ggagtgacca ccaactgcgc
     1681 caccaagagc gccaccaagg gccatgccac caatgcctgc catgagcatg tttcctacta
     1741 ccatccccgc aat
//
LOCUS       DATMTI010000070         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_681689, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000070 DATMTI010000000
VERSION     DATMTI010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681689"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VN039_01200"
     CDS             complement(<1..1101)
                     /locus_tag="VN039_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWV44639.1"
                     /translation="MTVSAAPIPDHPSNGSPAWLSTQEDISLILGASTEAIFITDQAG
                     HILFLNPAARQLVGRTRDVVGQGFHDLIDCLAADKREAVRCPFTRMVSTGEPVVIPSH
                     FWNREDGAQFELSLSFWPRTQQGVPVGSLVVVRDLTAAMEIQRDVQRAARLAEDAPNP
                     IAEFDATGAMLYANSGMLNVMGQCGLLEAGIEAMFPTNLSAILHECLASDSALSRVEH
                     VVADRVIAWSFFPLRELDLVRAYGLDITSDVALRRAKEAAEESARAKGIFLATMSHEL
                     RTPMNGVLGCTQLLRDTSLTGQQGELIETMHRSAEALLTLVNDILDFSKIEAGKMTLE
                     VADVNLRALIRDVTTLAEGLAAHKGLTLSVKID"
     regulatory      1397..1491
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1577..1930
                     /locus_tag="VN039_01205"
     CDS             1577..1930
                     /locus_tag="VN039_01205"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HWV44640.1"
                     /translation="MISAIHTALSGLTAIGKQIEVVAHNVANVNTDGFKKSRTEFVEI
                     PTGGVLPVVEKDDSSGPTVLRDTGGNQTMVELSNVDLGKEMVQQIVAQHSFEANLQTL
                     RTGDALLGSILDIRK"
     gene            2069..>4191
                     /locus_tag="VN039_01210"
     CDS             2069..>4191
                     /locus_tag="VN039_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWV44641.1"
                     /translation="MTQLNTPYPSTAYLTGFLQSRGIEAEQADLGIEMVLRLFSPDGL
                     QNVFRQLHGQEGSLPKEALGMMRGEQAYVKMIGPVVAFLQGKTPEIAARLIEPGLLPQ
                     GPRFRGRRKFERSVSVADRAKQWATFFLEDLADLVQATITPHFALSRYAEHIGRSASS
                     FDQIIAALAEPPSLTDEWLLDSFWEHVNRVNPSLVGLSIPFPGNLYGAFLVAKALKRH
                     RPDLPVAIGGGYVNTELRRVADPRVFDYIDFMTFDNGERPLLSLIEHISGSRPRRSLC
                     RTMYREKDRVIYADDPSSHDFTMNDIGCPTYRGLALDRYLTILDSTNPMHRLWSEGHW
                     NKLTVAHGCYWKQCTFCDIGLTYISRYEMTPTERLVRQIEQLVAETGRGGFHFVDEAA
                     PPSALKSLALALLERRIEITWWGNIRFETAFSPDLCRLLSASGCIAVTAGLEAASDRL
                     LDNMKKGITVDQTALVAAGFKDAGILIHAYLMYGCPSETIQETIDSLERVRQLVATDL
                     LQSAFWHRFTVTAHSPVGLDPDAHGLRILGPEFQGFAENDLLHHEGRGETPTWLGEGL
                     RRSLLNYLERRGLDLDVRQWFDEEVPLPQVPSTWLRRLLKKRTIDDRSDLERRLVWLG
                     GAVTCERIGKRATLTITGSRQDHVISLPEPEAQWLEQLIRDATPRQTCQTYPHMREAC
                     RRFPGIESTFNAFLATSSWKKIRGAG"
BASE COUNT          927 a         1230 c         1113 g          921 t
ORIGIN      
        1 atcgatcttc accgaaagag taagaccttt gtgggcggcc aatccctccg ccaacgtcgt
       61 gacgtcgcgg atcagtgccc gcagattgac atcggccact tcgagagtca tcttaccggc
      121 ttcgattttt gagaaatcga gaatgtcgtt caccaacgtc aacaacgctt ctgccgagcg
      181 atgcatcgtc tcgatcagtt ccccctgctg accggtcaac gatgtgtctc taaggagttg
      241 cgtgcatccc aacacaccgt tcatgggggt ccgaagctca tggctcatcg tggcgagaaa
      301 gatccctttc gcccgcgcag attcttcagc ggcttctttc gcgcggcgca aggccacatc
      361 ggaggtgata tcaagtccat acgcacggac tagatctaac tctctcaggg ggaagaacga
      421 ccaggcgatc acccgatccg ccacgacatg ttcgacccgc gacagagcgg aatcgctcgc
      481 gaggcactcg tgcagtattg ccgataggtt tgtcggaaac atcgcttcga tgccggcttc
      541 aagaagtcca cattgcccca tcacattcag catgccgctg ttggcataga gcatggcacc
      601 cgtcgcgtca aactctgcaa taggattcgg cgcatcctcg gccaaccgcg cggcgcgttg
      661 gacatcccgc tgaatctcca tcgcggcggt cagatcacgg acgaccacca ggctaccgac
      721 cggcacgcct tgttgcgttc ttggccagaa tgaaagcgat agctcgaact gagctccgtc
      781 ctctcgattc caaaaatgag aagggatcac gacaggttcg ccggtgctca ccattcgagt
      841 gaatgggcac cggaccgcct cacgcttgtc tgccgcaagg cagtcgataa ggtcgtgaaa
      901 cccctgtccg acgacatcac gcgttctgcc gacaagttgt cgcgcggcag gattgagaaa
      961 taggatgtga cctgcttggt ccgtaataaa gatcgcttct gttgaagccc cgagaataag
     1021 ggagatgtcc tcttgtgtcg acaaccaagc cggcgaaccg ttagaaggat ggtcggggat
     1081 gggtgccgca gaaaccgtca tacgctggcc tgcatcactg ttgcctcacc ggtacgacaa
     1141 agagccggtt atatcagtca taccttatcg gtacctacgg gtattttcta aatttagaaa
     1201 cctcgaacgg tcatgccgaa aacgaaccag agtcgcacgc catcactggt ttctggcgaa
     1261 caatcaaact ataccgtatg tgctcgattc tgccggttcg tggcggtcgc ccccggttgc
     1321 cacagatcgg tctctgctac acttcaatca acccgcgctc aagtcttcga tcaaatggcc
     1381 gatacagttt tcgacagggt gaaaaaggca aaccacccga gagggtggga cgcaaagccg
     1441 agggacctta acagggacgg aacatgttcc agggtcagcc tagctgccac acaaccggtg
     1501 atctgaccag tcaggctcct cccattcccc cggtgccttc ccctgaattc cagcctcaac
     1561 tgaatggagg tgccgcatga tttccgctat ccatacagca ctctcgggcc tgactgccat
     1621 cggcaaacag atcgaggtcg ttgcgcacaa cgtcgccaac gtcaataccg acggattcaa
     1681 gaaatccaga acagagttcg tcgaaatccc gaccggtggg gtcttgcctg ttgttgagaa
     1741 agatgactcc tccggtccaa ctgttcttcg cgataccggc ggcaatcaga ccatggtcga
     1801 gctctcgaac gtggatctgg gtaaggaaat ggtgcaacag atcgtagctc aacatagctt
     1861 tgaagcgaat ctccagacct tacgaacggg agatgcctta ctcggcagca tcctggacat
     1921 aagaaagtga aggctgaggc tcggatctcg gctttcgcct caaccgtagc ctcagcttag
     1981 tctcggacct tccgaatagc attctttctc acctttgtgc tatctcgaga ccatggcttg
     2041 tccccgtgtt ttgttgctga ttcctccttt gactcagctg aacacccctt acccatccac
     2101 cgcctattta accgggtttc ttcagtcccg tggaatcgag gcagagcaag ccgacttggg
     2161 catcgagatg gtgttgcgcc tattcagtcc cgatgggcta cagaatgtct tccgacaact
     2221 tcacggccag gagggctcac ttcccaagga agccctcggc atgatgaggg gtgaacaggc
     2281 ctacgtgaaa atgatcggac cggtcgtcgc cttcttgcag ggaaaaaccc ccgagattgc
     2341 cgctcgcttg atcgaacccg gactgttgcc ccagggcccg cgattccgag gtcgtaggaa
     2401 attcgagcgc tctgtatcag tggccgatag ggccaaacaa tgggcgacat tcttcctcga
     2461 agatctcgcc gatttggtgc aagctactat tacaccgcac tttgccttga gccgatatgc
     2521 cgagcatatc gggcgcagcg cctcatcgtt cgatcagatc atcgccgctt tggcggagcc
     2581 accgagtctg actgacgaat ggctcctgga ttcattctgg gaacacgtca accgagtcaa
     2641 cccgtcgctg gtcggtctct ccataccgtt tccgggtaat ctctatggag cattcctcgt
     2701 tgcaaaggcg ctcaagcggc accgtccaga tctgcctgtg gccatcggcg gcggctatgt
     2761 gaacacggaa ttgcgcaggg ttgccgatcc tcgcgtgttc gattatatcg actttatgac
     2821 gttcgacaac ggtgaacggc ccctcctctc cttaattgaa catatttccg gctcgcgtcc
     2881 gcgccgatcg ctgtgccgca cgatgtatcg cgaaaaggat cgcgtaatct atgccgatga
     2941 tccctcctca cacgacttca cgatgaacga catcggctgt ccaacctacc gcggcctggc
     3001 actggatcga tacctgacca ttctcgacag caccaatccg atgcaccgtc tctggtcgga
     3061 aggccattgg aataaactga ccgtggcaca cggctgctat tggaaacaat gtacattctg
     3121 cgatataggg ctcacctaca tcagtcggta cgagatgacg ccgaccgagc ggctcgtccg
     3181 gcaaatcgaa caactcgtag ccgaaacggg ccggggagga tttcacttcg ttgatgaggc
     3241 cgcaccacca tcagcattaa agtcgctggc ccttgccctc ttggagcgac ggatcgagat
     3301 tacctggtgg gggaatatcc gttttgagac cgctttttca ccggacctgt gccgccttct
     3361 ttccgcctcc ggttgtatcg cggtgacggc gggtcttgag gccgcctcgg accggctact
     3421 ggataatatg aagaaaggca ttacggtcga tcagaccgcg ttggtcgccg ccgggttcaa
     3481 agatgccggc atcctgatcc acgcctatct catgtatggt tgtccatctg aaacgattca
     3541 agaaaccatc gattccctcg aacgagtccg tcagcttgtc gcgacagatc tccttcaatc
     3601 agccttttgg catcgtttta cagtcaccgc tcacagcccc gtggggctgg atcccgatgc
     3661 tcatggcttg cgcattctcg ggcctgaatt tcaaggcttc gcagagaatg accttctgca
     3721 ccacgagggc cgaggggaaa cgccgacttg gctcggcgaa ggactgcgga gatcgctcct
     3781 gaactacctt gaacgacggg gactcgatct cgatgttcgc cagtggttcg atgaggaagt
     3841 gccgctccct caggtacctt ccacatggct cagacgcttg ctcaaaaagc ggacgatcga
     3901 tgaccgttca gacttagaac ggcgtttggt ctggctgggt ggagcagtga cttgtgagcg
     3961 gattggaaag agagcaaccc tcaccattac cggctccagg caggatcacg ttatttcact
     4021 cccggaaccg gaggcccaat ggttggaaca attgattaga gacgctaccc cgaggcagac
     4081 atgtcagacc tatccgcata tgcgagaagc ctgccgacgg tttcccggca tcgagtcgac
     4141 gttcaacgcc ttcttggcta cctcttcgtg gaagaaaatc cgcggtgcgg g
//
LOCUS       DATMTI010000071         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_342323, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000071 DATMTI010000000
VERSION     DATMTI010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342323"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VN039_01215"
     CDS             <1..204
                     /locus_tag="VN039_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HWV44642.1"
                     /translation="KIDKKLVQRINDLIKEITRTPHTGTGKPEPLKHALAGYWSRRIN
                     EEHRIVYKVVDDSVLIAQLRYHY"
     gene            complement(381..2306)
                     /gene="glgB"
                     /locus_tag="VN039_01220"
     CDS             complement(381..2306)
                     /gene="glgB"
                     /locus_tag="VN039_01220"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWV44643.1"
                     /translation="MTQQAIMTVDEPTRLTRDDLYLFNEGSLVNLYEKLGTHAGRVNG
                     QDGTFFAVWAPDAERVSVIGSFNDWKQDAHYLNPRGSSGIWEGFIPGIGVGTLYKYHI
                     RSRFNGYRVDKTDPLSFFNEIPPKTASIVWDLAYEWNDAEWMRQRRDRNALTAPMSIY
                     EMHVGSWRRVPSEGHRSLSYREMAVPLAEYIQQTGFTHVEFLPLMDHPFFGSWGYQTT
                     GYFAPSGNYGTPQDLMYLIDTLHQHGIGVILDWVPSHFPTDEHGLGYFDGTHLYEHAH
                     PKQGFHPEWNTFIFNYGRNEVRSFLISSALFWLDKYHIDGLRVDAVASMLYLDYARKE
                     GEWIPNQYGGRENLDAVAFLRRFNEEVYMRYPDVQTTAEESTSWPAVSRPGYVGGLGF
                     GLKWDMGWMHDTLKYMQTDPIYRHYHHQNLTFRMLYAFHENFVLPLSHDEVVYGKRSL
                     LEKMPGDDWQKFANLRVLFGYMYAQAAKKLLFMGGEIAQRAEWAHDGQLEWEQLQHAS
                     HLGIQRWVADLNRVYRTERALHEDDVTPHGFEWIDCNDAESSVISLIRKGHTMQDIIL
                     VVCNFTPVPRWNYQVGVPRGGYWREILNSDSSWYGGGDWGNGGGVEAAPVPLHARSHS
                     LTVTVPALAALFFKSQG"
     gene            complement(2390..>2920)
                     /locus_tag="VN039_01225"
     CDS             complement(2390..>2920)
                     /locus_tag="VN039_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44644.1"
                     /translation="LESKALLLSRLEAPVKRNTQAVRIRCHGDFHLGQVLYTGRDFVI
                     IDFEGEPARPLAERRAKHVPIVDVAGMIRSFHYAASVALDRVGSRPDADERRSKMELR
                     ARQWYRSATDAFLTGYAETAGKSPFLPEKSEDRDMLLGAYLIEKACYELSYELNNRPS
                     WAGIPLSGLLQLAQPT"
BASE COUNT          671 a          887 c          745 g          617 t
ORIGIN      
        1 aagatcgaca aaaagctggt gcagcgcatc aatgacctta tcaaagagat cacccggacg
       61 ccccacacag gcacaggaaa gcctgagccg ctcaagcacg ccctggcagg ttattggtcg
      121 cgccgtatca acgaagaaca ccgcatcgtt tacaaagtgg tcgatgattc agtgcttatc
      181 gctcagctgc gatatcacta ctgagggtac ctaacgcctt ggcgttgagc cgccgacgcc
      241 taggaccgcc cacatgaacc tgccataatt tcgaaggaca cgatgctatt gggattatga
      301 attgaattgg ggtcaagcct tgttctgtgc ataccgggaa gcataacctc cgtccataat
      361 tcatcattca gaatttaaga ctagccctgg ctcttgaaaa acaacgccgc cagggcggga
      421 acggtcaccg tcaatgaatg ggaccgcgca tgcagcggca ccggtgcggc ttcgacgcca
      481 cctccattac cccagtcgcc acccccatac caggaagaat cactgttgag aatttcccgc
      541 cagtatcccc cacgcggaac ccctacctga taattccaac gcggcaccgg cgtaaaattg
      601 cacaccacca agataatgtc ctgcatcgta tgccccttgc ggatcaggct gatgacgctc
      661 gactccgcat cgttgcagtc gatccactca aatccatggg gagtcacatc gtcctcatgc
      721 agggcccgtt cggtccgata gacacggttg agatccgcaa cccatcgctg aatacctaaa
      781 tggctcgcat gctgcagttg ctcccattcc aattgaccgt cgtgggccca ttccgcgcgc
      841 tgagcaatct ctccacccat gaagagcagc tttttggccg cctgcgcgta catgtacccg
      901 aacagcacac ggagattagc gaacttctgc cagtcgtctc ccggcatctt ttcgagcagc
      961 gaacgcttcc catacaccac ttcgtcatga gacagcggca agacgaaatt ctcgtgaaac
     1021 gcgtagagca tgcgaaacgt gagattctga tggtgataat gccggtagat cggatcggtc
     1081 tgcatatatt tcaaggtgtc atgcatccag cccatatccc acttcaggcc gaagcccagc
     1141 cccccgacgt agcccgggcg cgagacggcc ggccaggacg tggattcctc cgccgtggtc
     1201 tgcacgtccg gataccgcat atagacttcc tcgttgaatc gccgtagaaa ggcgaccgca
     1261 tccaggttct ctcgcccacc gtactggttc ggaatccatt caccttcctt gcgcgcataa
     1321 tccaagtaca gcatcgacgc gaccgcatcg acccgcaggc cgtcgatatg gtacttgtcg
     1381 agccaaaaca gcgcgctgct gatcaagaaa ctgcgcacct cattacggcc atagttgaag
     1441 atgaaggtgt tccactccgg atgaaagccc tgcttcggat gcgcatgctc atataggtgc
     1501 gtcccgtcga aatagccgag tccatgctca tcggtgggaa aatgcgacgg tacccagtcc
     1561 aggatgaccc cgatgccgtg ctggtgcagg gtgtcgatca agtacatgag atcttgaggg
     1621 gtaccgtagt tgccggaggg cgcaaaatac ccggtggttt gatagcccca tgagccgaag
     1681 aagggatggt ccatcagcgg aaggaactcc acgtgggtga agcccgtctg ctggatatat
     1741 tctgccagcg gtacggccat ctcgcgataa ctgagggacc gatggccttc cgacggcaca
     1801 cgcctccatg acccgacatg catttcatag atcgacatgg gcgccgtgag cgcattccgg
     1861 tctcgtcgct gccgcatcca ctccgcatcg ttccactcat aggccaagtc ccaaacgatc
     1921 gaagcggtct tcggtggaat ctcgttaaaa aacgagagcg ggtcggtctt gtccacgcga
     1981 tagccgttga agcgcgagcg aatgtggtac ttgtacagag tgccgactcc gatgccggga
     2041 atgaaccctt cccaaattcc gctgctccca cgagggttca gatagtgcgc atcttgtttc
     2101 cagtcgttaa acgagccgat cacggacacc cgctcggcat ccggggccca gaccgcaaag
     2161 aatgttccat cctgtccgtt cacgcgcccc gcgtgcgtgc ccaatttttc atacagattg
     2221 accaatgatc cctcattaaa gagatagagg tcgtcgcgcg tcagcctcgt cggttcatcg
     2281 acagtcatga tcgcttgctg tgtcatccgt cactccatag ttctgaattc tgaaacctga
     2341 atcatggtga gcatctcata ctaatccgca ccaatcgacc agtcagaatt catgtaggtt
     2401 gtgcgagctg aagtagtccg ctcagtggaa ttccggccca cgaaggccgg ttgttcagct
     2461 catagctcag ctcataacag gccttttcga tcaaatatgc ccccagaagc atgtcgcggt
     2521 cttcggattt ctccgggagg aacggagatt tgcccgctgt ttcggcatac cctgttaaga
     2581 atgcatccgt cgccgaccga taccattgcc tcgccctcag ttccatcttg gatcgtcgtt
     2641 catcggcgtc cggccggctt ccaacccgat caagcgccac ggaagcggca tagtgaaatg
     2701 atcggatcat ccccgccaca tcgacgatcg ggacatgttt cgctcgccgc tccgccaacg
     2761 gcctggccgg ttcgccctca aaatcaatga tcacgaaatc ccggcccgtg tacaggacct
     2821 ggcccaaatg aaaatcaccg tgacatcgaa ttctgacggc ctgggtattc cgcttgaccg
     2881 gggcctccag cctggacagc aatagggctt tcgattcgag
//
LOCUS       DATMTI010000072         7908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_427129, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000072 DATMTI010000000
VERSION     DATMTI010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427129"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="traF"
                     /locus_tag="VN039_01230"
     CDS             complement(<1..896)
                     /gene="traF"
                     /locus_tag="VN039_01230"
                     /inference="COORDINATES: protein motif:HMM:NF025106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HWV44645.1"
                     /translation="MRRLTLLCTAVLLVASTLPPHVSASEFVLAGPRAMGMGGAGVAV
                     TTNALATYWNPAGLAMTQTVDIRAQGGGQATDRLGLGDALHDLENFSTGDTSPANLAK
                     GQSIADRINRSGAAVSVNGSAGLYVKGHLGEHAFGFNVSDVATGGGFVSTPVQASQPG
                     GAGTPITVAGQMALRGLEARQVAFSYAYAFSDKTFSIGITAKIIQGAAYNGSTDLQGG
                     SGVSTIDHFGKPNISTTYGIDIGAIYRPSSWIRFGIVAKDINKPTFDAADGGDLKLEP
                     QVRAGIAINPYSSLTLTADVDAT"
     gene            complement(1082..1744)
                     /locus_tag="VN039_01235"
     CDS             complement(1082..1744)
                     /locus_tag="VN039_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV44646.1"
                     /translation="MIKVTNLHKSFSMGSYELPVLRGINLKIQRGELVAIVGASGAGK
                     STLLHIIGTLDKPSSGTVTFDEHDLFRMTDTQQAEFRNRRIGFVFQFHHLLAEFTALE
                     NACMPALVQRREPISVKADATTLLTEVGLGQRLHHKPGELSGGEQQRVAMARALMQNP
                     DLVLADEPTGNLDTHSGDGLFGLMRTLNKTRGTTFVIVTHNDKLSAQSDRIIHMQDGQ
                     II"
     gene            complement(1844..3121)
                     /locus_tag="VN039_01240"
     CDS             complement(1844..3121)
                     /locus_tag="VN039_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HWV44647.1"
                     /translation="MTLPYEIFVGLRYLRAKRRNRTISLNTFVSVAGITLGVAALIGT
                     VGIMTGFREDIQSKILGTTAHIVVQERMKEHMTDYDRLTDKIQTVPDVVAATPFVFRQ
                     VLLTTQSGVQGIILRGIDPKREANVTELAKNITAGQLLDLLAPVKVMQPPADDPQGDL
                     RPVEKPGIILGKELALRLGVFVGDTVNVVSPVGPISAMGMVPKIRTFAVVALFHSGMF
                     EYDSSFAYIELGEAQKFFNLGASVTGIEVKVTDVFRAGEIAHNIEQELGFSHGARDWM
                     QMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQKEIAILKAMGATKR
                     SIRRIFMLNGLIIGFAGTGIGIPLGYAFLWLIQTFWTFDPSVYYISTIPVHVLAEDVL
                     LVAGSAILISFLATVYPANQAAKLEPVAALRYE"
     gene            complement(3121..4605)
                     /gene="lysS"
                     /locus_tag="VN039_01245"
     CDS             complement(3121..4605)
                     /gene="lysS"
                     /locus_tag="VN039_01245"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWV44648.1"
                     /translation="MDELNEQRQQRIKKLDLLREAGVAPYGRRFEAKDRAGQLLTLHG
                     EKTKETLEQEKVSCTVAGRVVALRRFGKAGFAVLQDGSDRLQVYLKKDLLSEQAYTVV
                     EQLDLGDWIGVSGTLFRTKTNEFTVEVHHLTFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPLVHQIFSTRSRIITGIRTFLMERGFLEVETPMMHPIPGGANAKPFVTHHNA
                     LGVDLYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVSYADF
                     QDLIVLTEELVSSLVQQILGKTVIPYQGKEIVLTPPWRRWSYHQAVLEVNKLDPSVLR
                     DREQALAAAKRLNVPVDPKASLFAIINEIFEETVEPNLQQPTFITDYPIEISPLAKRK
                     ETDPSLTDRFELYIAGREIANAFSELNDPLDQRERLEGQAAQREAGDEEAHLVDEDFL
                     RALEFGMPPTAGEGIGIDRLIMLFTDQASIRDVVLFPQLRPEKS"
     gene            complement(4627..5773)
                     /gene="prfB"
                     /locus_tag="VN039_01250"
     CDS             complement(join(4627..5703,5705..5773))
                     /gene="prfB"
                     /locus_tag="VN039_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWV44649.1"
                     /translation="MLEDVEVRVRALAEQVSELRGHLDFAHMTADLQELEAQMGQPHF
                     WSDARTAATVSRKKATIERELQQWRDIETKMGDLDALLELAHESGDSALETELAGELN
                     QLEPRLATLRVELLLSGELDPNNAIVAIHPGAGGTESQDWAQMLLRMYVRWAEHKKFK
                     VETLDLLPGDEAGIKSVTFSITGPYAYGYLKAEAGVHRLVRISPFDSNKRRHTSFASV
                     FVYPELSEDIDVVIEEKDLRIDTFRSGGAGGQNVNKVETAIRITHLPSGIVVQCQNER
                     SQLQNRNGAMKILKARLFELEQKKKEAEFNAIVGEKKDIAWGSQIRSYVFQPYQLVKD
                     HRTGHQISNVSSVMDGGLDEFIEAFLKKKLGKGSDHLAPVGGPEDDL"
     gene            complement(5795..7411)
                     /gene="lnt"
                     /locus_tag="VN039_01255"
     CDS             complement(5795..7411)
                     /gene="lnt"
                     /locus_tag="VN039_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWV44650.1"
                     /translation="MDRARSRQILLACASGLLLPFCFPRFDLGLLAWIVLIPLHLALD
                     QCSRQRAFWIGWLSGLVGFTGIMAWVVTAMTTYGKVPEPVSYAILLLLATYLGLYVGL
                     YCLAVVWLRELIPRYGIFFAPCFWVALEWFRTHLLSGLPWCLLGYSQYRELDLIQVAD
                     HTGVYGISFLIVLVNVAFAELFLWIMPFFRGFHPVQLPWELLTTAAACMVLSWFYSSA
                     VLSARDLGHHKPSVTIGVVQPNIDQAVKWDASFRDETMRRFDRLTAQLGTSNDLVVWP
                     EAATPFIFEREKEYQLQLITLAERAQAPILLGSPALRFYPDRRPYLLNSAYLLGSDGT
                     VLGRYDKHHLVPFGEYIPLKSSLLFFLDKLVEGIGDFEAGPGPTTLSFNPKSWSKERS
                     AGSRSVKFGVAICYEVIFPDLVRQFAMNGAEFLVTITNDGWFGPSSAPAQHFAMVVFR
                     SVENHLAFARAANTGISGFVDPYGQIIEATPIFTEEASSATIQTRQARTFYSYYGDVF
                     AHACVIICALLILFGYFRTKEPALTAITPA"
     gene            7526..7855
                     /locus_tag="VN039_01260"
     CDS             7526..7855
                     /locus_tag="VN039_01260"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV44651.1"
                     /translation="MERRRHRRVPAQVKSLLRANSHEVEGEVLDLSLGGALVESSLEV
                     QPGKQIAVKLIVPGDDTPILIEQARVQWTVDRTFGVRFVDVQHQEMDELEQLIDECIA
                     LDEGRET"
BASE COUNT         1727 a         2193 c         2255 g         1733 t
ORIGIN      
        1 gtcgcatcca catcggccgt caatgtcaga gaagagtacg gattgattgc gatgccggcc
       61 cgaacttgcg gttccaattt caagtcccct ccgtccgcag catcgaaggt aggcttattg
      121 atgtcctttg cgacgatccc aaacctgatc cacgaggatg gacgatagat tgctcctatg
      181 tcaattccat aggtcgttga gatgttcggt ttgccgaaat gatcgatcgt gctgacaccg
      241 ctcccacctt ggaggtcggt agagccgttg tatgcggcgc cttgaatgat tttcgcggta
      301 attccgattg aaaaggtttt gtcggaaaag gcataggcat aggagaaggc cacctgtctg
      361 gcttcgaggc cacgaagcgc catctggccc gctacggtaa tgggtgtccc cgctccgccc
      421 ggttgggatg cctgcaccgg tgtcgagacg aatccccctc cggtagcgac gtcagacaca
      481 ttgaacccga acgcatgttc accaaggtgt cctttgacgt acaggccggc ggatccgttg
      541 acggaaacag cggccccaga tcggttgata cgatcggcga tcgactgacc cttcgccaga
      601 ttggccgggg acgtgtcacc agtgctgaag ttctccaggt catgcagcgc atcacccaag
      661 ccgaggcgat cagtcgcttg cccgcctcct tgggcgcgga tgtccacggt ttgggtcatg
      721 gccaagccgg cggggttcca ataggtggcg agtgcattgg ttgtgacggc tactccggcg
      781 ccacccatgc ccatggcacg agggcccgcg agcacgaatt ctgaggccga gacgtgagga
      841 ggcaaggttg atgcgaccag caaaacggct gtgcaaagca acgtcaaccg gcgcatgtcc
      901 tgtcctcgat tcagtaaggt tttgagtcta gagaacgaat aacggttcgt caaggaatcg
      961 cagatagcct aacaggtgca ggccgaacct ccgtgcggcg agaaggttga aggcatgctc
     1021 ggaaaatatc gggaccaatt ggcacgagcg ggatgctctg ataaagagtg gccggctccg
     1081 atcaaatgat ttgcccatcc tgcatatgaa tgatgcggtc ggattgggcg gagagtttgt
     1141 cattatgggt cacgatcacg aaggtggttc cgcgtgtttt gttcaaggtg cgcatcaatc
     1201 cgaacaatcc gtccccgcta tgggtgtcaa gatttccggt cggctcatcg gccaagacca
     1261 agtccggatt ctgcatcaac gcgcgtgcca tcgcgacacg ttgttgctcg ccgcctgaga
     1321 gctctcccgg cttgtgatgg aggcgctgtc ccaatccgac ttccgtgagc agggttgttg
     1381 catcggcctt gaccgaaatt ggttcgcggc gctgtacgag cgccggcatg caggcatttt
     1441 ccaatgcggt gaactcggca agcaggtggt gaaattgaaa cacgaatccg atccgccgat
     1501 ttcggaattc cgcctgctgt gtgtccgtca tccgaaagag gtcatgctca tcgaacgtca
     1561 ccgtaccact gctcggctta tcgagcgttc cgataatatg gagcaaggtg ctcttgccgg
     1621 ctcctgaagc tcccacgatc gccaccagct cgccgcgctg aatcttcaga ttgattcccc
     1681 tgaggacggg caattcatag gatcccatcg aaaaagattt atggaggttc gtgaccttaa
     1741 tcattccgat attcttaatc tcagtccttc gtccttgtta ctcggcactt gagtatgtaa
     1801 ttctcggctc agtcctcgac cctcaatcct cagtcctgcg aatctattca tagcgcagcg
     1861 cggcgacggg ttccagcttc gcggcctgat tggccggata gaccgtcgcc aaaaagctga
     1921 tcagaatggc ggacccggcc acgaggagca cgtcctcggc cagcacgtga acaggaatgg
     1981 tggagatgta gtacacgctc ggatcgaacg tccaaaatgt ttgaatcagc cagagaaacg
     2041 catagcccaa gggaatgccg atgcctgtac ccgcaaatcc gatgatcaat ccgttcagca
     2101 tgaagatgcg cctgatactg cgcttcgttg cgcccatcgc cttgaggatc gcgatttctt
     2161 tctgcttctc cgtcacgatc atcgtcaagg tgctgacgat gttgaatgag gccacgatcg
     2221 tgatcaagac aagaaggaga aacatcatcg tcttctccag cttcagggcc gagaagagat
     2281 tgcggttcat ctgcatccaa tctctggctc catgactgaa tcccagctcc tgttcaatgt
     2341 tgtgggcgat ctctccggcc cggaacacat ccgtgacctt gacttcgatt ccggttacgc
     2401 tcgcgcccag gttgaaaaac ttctgtgctt cgccgagttc gatataagca aatgaagaat
     2461 cgtactcgaa cattcctgaa tggaacaagg cgaccacggc gaacgtcctg atcttgggca
     2521 ccatccccat cgcgctgatc gggccgacgg gagagaccac gttgaccgta tcgccgacga
     2581 acacgcccaa cctgagcgcc agctccttgc cgagaatgat gccgggtttt tcaacggggc
     2641 ggaggtcgcc ttgagggtca tcggccggag gctgcatgac tttcaccggg gcgagcaggt
     2701 cgagcagctg tcccgccgtg atgttcttgg cgagttcggt gacattggct tctcgttttg
     2761 gatcaatccc gcggaggatg attccttgta ccccgctctg cgtggtgagc aatacttggc
     2821 ggaatacaaa cggcgtggct gccacgacgt cgggcaccgt ctggatcttg tcggtcagac
     2881 ggtcatagtc cgtcatgtgt tctttcattc gttcttggac gacgatatgt gcggtcgtgc
     2941 cgagaatctt gctctgaatg tcctcccgga agccggtcat gatgccgacc gtaccgatca
     3001 gcgcggccac ccccagagta atcccggcaa ccgacacgaa ggtgttcagc gagatggtcc
     3061 ggttacggcg cttggcgcgc aggtagcgaa gtccgacgaa gatttcatag gggagagtca
     3121 ctacgatttc tctggtctga gctgtgggaa aagcacgacg tcgcggatgg aagcctgatc
     3181 ggtaaacagc atgatcaatc ggtcgatccc gatcccttct ccggcggtcg gcggcattcc
     3241 gaattcaagg gcgcggagaa agtcctcgtc gacgagatgg gcttcttcat cacccgcctc
     3301 ccgctgagcc gcctggcctt ccaaccgttc acgttggtcc aacggatcgt tcaattcgga
     3361 aaaggcattc gcgatttcgc gcccggcaat gtacagctcg aatcgatcgg tcaggctcgg
     3421 gtccgtctcc ttccgctttg cgaggggaga aatttcgatc gggtaatcgg tgataaaagt
     3481 gggctgttgt aaattaggtt caacggtttc ctcgaaaatc tcgttgatga tcgcgaacaa
     3541 cgatgccttc ggatctaccg gcacattcag ccgtttggct gccgccaatg cctgctctcg
     3601 atcgcggagt accgatgggt cgagtttatt cacctctagg acggcttggt ggtaagacca
     3661 tcgtcgccat ggaggcgtga gaacgatctc ttttccttgg tacggaatga ccgtcttgcc
     3721 aagaatctgc tgaaccaggc ttgagacaag ttcttcagtg agtacgatca gatcctggaa
     3781 atccgcgtat gacacgtaga actccagcat cgtgaattca gggttgtgga tcgtcgatat
     3841 accctcattc cggaagttgc ggttgatctc gaacacgcgc ggaaaaccgc cgacgatcag
     3901 gcgtttcaaa tacagctccg gcgcgatacg caagtaaaga tccacgccga gtgcgttatg
     3961 gtgcgtgacg aagggtttgg cgttcgcacc tcctggaatc ggatgcatca tcggggtttc
     4021 gacctcgagg aacccccgct ccatgagaaa cgttcggatg ccggtaatga tacggcttct
     4081 ggtcgaaaaa atctgatgga ccagggggtt ggcaatcaga tcgacataac gttgccgata
     4141 cctggtttca acgtccgtca gcccatgcca cttttcgggg agcggccgga gagctttact
     4201 gagaaacgtc aggtgatgga cttcgaccgt gaactcgttc gttttggtac gaaacaatgt
     4261 cccgctgacg ccgatccagt cgccgagatc aagctgctcc acgacggtgt aggcctgttc
     4321 ggagagcaga tccttcttga gatatacttg gaggcggtcg gacccgtcct gcagcacggc
     4381 aaacccggcc ttaccgaaac gccgcaaggc gacgacccgt cccgcgactg tgcaggagac
     4441 cttttcttgc tccaaggtct ccttggtctt ctcgccatgc agcgtgagta attgaccggc
     4501 ccgatccttg gcctcgaagc gcctgccgta gggggcgacg ccggcctctc gaaggagatc
     4561 gagtttcttg attcgctgct gccgctgttc gttcagttcg tccattctta cccttcggca
     4621 atcctgctac aggtcgtctt ccggtccgcc gaccggtgcg aggtgatcgc ttccctttcc
     4681 caatttcttc ttcaaaaagg cctcgatgaa ttcatcgagg cctccgtcca tgacggatga
     4741 gacgttgctg atttgatgac ccgttcggtg atccttgacc agttggtaag gctggaacac
     4801 gtaggatcga atttggctgc cccatgcgat gtccttcttt tcgccgacga tggcgttgaa
     4861 ctccgcttcc ttcttcttct gttccaattc gaagaggcgc gccttcaaga ttttcatcgc
     4921 cccgtttcga ttctggagct gggaccgctc attctggcat tgcacgacga taccactcgg
     4981 caagtgcgtg atgcggatgg cggtttccac cttattgaca ttctgacctc ctgcgccgcc
     5041 cgaccgaaag gtgtcgatcc ggagatcttt ttcctcgatc acgacatcga tgtcttcgct
     5101 cagctccgga tacacgaaga ctgatgcgaa cgacgtgtgt cgccgcttgt tggagtcgaa
     5161 tggggaaatg cgcaccaagc gatgtacccc ggcttccgct ttcaggtatc cataggcata
     5221 gggccccgtg atggagaacg tcacgctttt gatgcccgct tcgtcgccgg gcaacagatc
     5281 cagggtttcc accttgaatt ttttgtgttc cgcccaccgc acatacatcc gaaggagcat
     5341 ttgtgcccaa tcctgcgatt ccgtgccgcc ggcaccggga tgaatcgcca caatggcatt
     5401 attggggtcc agttctcccg agaggagaag ctcgacacgc agtgtggcga ggcgaggctc
     5461 cagctgattc agttcaccgg ccagctcggt ctccagagcc gagtcgccgc tctcatgggc
     5521 aagctccagc agcgcatcca aatcgcccat tttggtctcg atgtcgcgcc actgctggag
     5581 ctctcgctcg atcgtcgcct tcttccggct caccgttgca gcggtgcggg catcgctcca
     5641 gaagtgaggc tggcccatct gcgcttcgag ctcttgcaag tcggccgtca tatgagcgaa
     5701 gtcaaagatg cccccgtagt tcagagactt gttccgccag ggcacgcacc cgaacttcca
     5761 cgtcctctaa catgtcacgg tcctccttct tcgttcatgc cggtgtgatt gccgtcaggg
     5821 ctggttcctt cgtgcggaag tacccgaaca gaatcaaaag cgcgcagatt ataacacagg
     5881 cgtgggcaaa cacatcgccg taatagctgt aaaacgtgcg ggcttgtctg gtttggatcg
     5941 ttgcagagga cgcttcctcc gtaaaaatcg gcgtcgcttc gatgatttgc ccgtacggat
     6001 cgacgaagcc ggagattccc gtgttggcgg cgcgcgcgaa ggccaaatga ttttccaccg
     6061 agcgaaagac gaccatggca aagtgctggg cgggggccga ggatggtcca aaccatccgt
     6121 cattcgtgat cgtgaccaga aactccgcgc cgttcatggc gaactgacgg accaaatccg
     6181 gaaagatcac ttcgtagcag atcgccacgc caaacttgac cgatctggag cctgccgaac
     6241 gttctttgct ccaggatttc gggttgaacg aaagagtcgt cggcccaggt cctgcttcga
     6301 aatcgccgat cccttcgacg agtttatcga ggaagaacag gagcgaggat ttgagcggga
     6361 tgtactctcc aaacggcacg agatggtgct tatcataacg gccgaggact gtgccgtcac
     6421 ttcccagcag ataggcgcta ttcaagaggt aggggcggcg atcaggatag aatctcaagg
     6481 ccgggctgcc gaggaggatc ggcgcctgcg cgcgttccgc caaagtgatc agctgcagct
     6541 gatactcttt ctcccgctcg aaaataaacg gggtggctgc ctcgggccac acgaccaaat
     6601 cgttactggt tcccagttgg gccgtcagcc ggtcgaaccg tcgcatcgtc tcgtcgcgga
     6661 atgacgcatc ccacttcacg gcttgatcga tattcggctg aacgacaccg atggtaacgg
     6721 aaggtttatg atggcccaga tctctagcac tcaggactgc tgaactatag aaccatgaga
     6781 gcaccatgca cgcggccgcg gtggtgagca attcccacgg aagctggacc gggtgaaatc
     6841 ctcggaaaaa cggcatgatc cacaagaata gctcggcgaa ggctacattc accaggacga
     6901 tgaggaagga aatgccgtac acgcccgtat ggtcagccac ttgaatcagg tccaattcgc
     6961 gatattgtga atagccgagg agacaccatg gaaggccgga gagcaggtgg gtacggaacc
     7021 attcgagtgc gacccagaaa cagggggcaa agaaaattcc atagcgtggg atgagctcgc
     7081 gcaaccatac aacagcgagg caatagagcc cgacatagag tccgaggtag gtggccaaca
     7141 gcaacaaaat tgcgtaactg acgggttcgg ggaccttgcc ataggtcgtc atggcggtga
     7201 cgacccaggc catgatgccg gtgaatccga ccagaccact caaccagccg atccaaaagg
     7261 cccgctgtct ggagcattga tcaagcgcaa gatgaagggg aatgaggacg atccaggcca
     7321 gcaatcccag gtcaaaccgt ggaaaacaga aaggcaaaag cagtccgctc gcgcaggcga
     7381 ggaggatctg gcgcgaacga gctcggtcca tcgtgccgta ccttatcgaa aagattcgat
     7441 gacttgcaag agaatgcttg aacaatcagc gacttgggag accggccacg tgcttttccc
     7501 cggtgaaaaa ggtattctat cttccatgga gcgacgtcgt caccgtcgag tgccggctca
     7561 agtcaagagc ttgcttcggg caaattcgca tgaagtggaa ggagaggtcc tcgatctgtc
     7621 gcttggcggc gccctggtgg aaagctctct tgaggtccaa ccaggaaagc agattgccgt
     7681 caagctgatt gtcccaggag atgatactcc gattctgatt gaacaagctc gcgtacaatg
     7741 gaccgtcgat cgaacgttcg gggttcggtt tgtggacgtg caacatcagg aaatggatga
     7801 gttggagcag ctgatcgatg aatgcatcgc gcttgatgaa gggagagaga catgagactc
     7861 gtgagcaatc caactgagac cccgaccgat gacccccgcc gctggatc
//
LOCUS       DATMTI010000073         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_384767, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000073 DATMTI010000000
VERSION     DATMTI010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384767"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..613)
                     /locus_tag="VN039_01265"
                     /pseudo
     CDS             complement(473..613)
                     /locus_tag="VN039_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558212.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            784..873
                     /locus_tag="VN039_01270"
     tRNA            784..873
                     /locus_tag="VN039_01270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:818..820,aa:Ser,seq:gga)
     gene            982..1081
                     /gene="ffs"
                     /locus_tag="VN039_01275"
     ncRNA           982..1081
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN039_01275"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1191..>2757
                     /gene="dnaX"
                     /locus_tag="VN039_01280"
     CDS             1191..>2757
                     /gene="dnaX"
                     /locus_tag="VN039_01280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWV44652.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQPHVVQTLMNAVSTKRIAHAYLFS
                     GTRGVGKTTVARILGKALNCERGPTSHPCNTCGNCLEIMQGNSVDVIEIDGASNTSVD
                     DVREIRENVKFTPFRGQFRVYIIDEVHMLSNSAFNALLKTLEEPPTHVVFIFATTEIH
                     KIPATILSRCQHYNFRRIARTEIIERLRHVAAQDRLTLEERSFAALARASEGSMRDAL
                     SLLDQAVAYGGKTISHADLELLLGAVPQELVQELIRAIMAQDSPAALASLANLLDRGH
                     DLRAFCAEIVEHIRNLLVAAVVPGTVELRSLIEISEDDLNQLSMNAKTLTPEQLQELL
                     AIFIQAEDALRFTNHPRFVMETAAVRATRLLRQREGIAARPVQTASSSTQKPPVEPEG
                     RKSGPSTPPALRQNGPVASRPSPKPTQTSRAETEGGRSISSEPSHDPPTDVATATPAA
                     VPPLAVDPQPTLHWELVQEEVAASFPNIAPFLEAGRLVGMEDGIVTIGFAKQATVARA
                     RLEKEENLLVLSKL"
BASE COUNT          629 a          772 c          736 g          620 t
ORIGIN      
        1 ctagagtagg aagggttacc ttggaacgct ggaccgacgt gcaactggtc aaggtgatcg
       61 ttgtcatctt ggtcattatc tggggagttg cgatgttaat gaacggccct cttgatcgcc
      121 tcctcggctt cttcggtatg agtttctccc ctcactaata cgtcagaaat tgcagagcca
      181 tcccttgaaa tgttatgacg atcacgtagg acgccgattg tcagccatcg gccttcttca
      241 ctaactcaat catatccgcc gcatcttttg gtagctcgcc cacgatcttg tcggtcatag
      301 ggtcggcttg tccttcgcca ctcaggtgtc ggacgatctc gtttgcctgg caacaccgta
      361 gcccatctgg gcttgaagtg ttgcacttgg agtaggactc aaggatgaaa gacgagaaac
      421 tcgaagaccg tgatggacga aagagaatcc aaaccctagg ctctgtcgac atttacttga
      481 gccatacgta actggtcgcg agctggacga acgacaggaa ggagagcgcg ggcgtgtcgt
      541 accgcgttgc cacccgacgg aaatgcttga ggtacccaca catccgttcc accagattcc
      601 gctccttata cagcgcgttg atcctctcgc acatgcctcg gctcgtccat gagaccctcc
      661 ttggtgagaa gattctcgag cacttttcgc taaatgtcga cagagcctaa ctacacggtg
      721 gtcctcacct tgccggtcat ggacacgcat gctaagatgc tatccctttg cacttctcga
      781 tgcggagagg tgcgagagtg gacgaatcga catgcttgga aagcatgcgt cccggcaacg
      841 ggaccgtggg ttcgaatccc accctctccg ccataccgca cttcgtgtgt accgtccgct
      901 cgttattgct gtatcgtaat ttctgcgggc ggatagacac ctcaagtgtg gactgtattg
      961 gaggtctaga tcgagcatag gggctgggcc ctgtgcaata gaaccctgtg aaccccgcca
     1021 gatccggaag gaagcaacgg taagcagcca gttctgggtg ccgcaggatc acctggcccc
     1081 actttggacg tgaggcgtga acatgaggcg taaggcgaca gtatcttcgg catcatctcg
     1141 tcttgcttta caccccttac attttacccc tcatccctga cggcttcttt atggattatc
     1201 aagtctccgc gcgtaaatac cggcccggta cgtttgacga tgtgatcggt cagccccatg
     1261 tcgtccaaac gttaatgaac gcggtctcga cgaagcggat cgcgcatgcg tatctatttt
     1321 ccggcacacg aggcgtgggg aaaacgaccg tcgcacgaat tctcggaaag gcactcaact
     1381 gtgaacgggg accgacaagc catccctgca atacctgcgg gaactgcctc gagatcatgc
     1441 aagggaattc agtcgatgtc atcgaaattg atggtgcgtc caacaccagc gtggacgatg
     1501 tgcgagagat ccgcgagaac gttaaattca cgccgtttcg tggtcagttt cgagtctaca
     1561 ttatcgatga agtccacatg ctctcaaact cggcgtttaa tgccctattg aagacactcg
     1621 aagaaccccc aacgcatgtg gttttcattt ttgccacaac ggagattcat aaaatccccg
     1681 cgacgattct ctcgcgatgc cagcattaca actttcgtcg cattgccaga accgaaatca
     1741 ttgagcgact tcggcacgtg gcagcgcaag atcggttgac gcttgaggaa cggagcttcg
     1801 cggccctggc ccgcgccagt gaaggaagca tgcgcgatgc cttgagtttg ctcgatcagg
     1861 ccgttgcata tggcggcaag accatcagcc acgcggatct cgaactgctg ttgggagcag
     1921 tacctcaaga actggtgcag gaactcatta gagcgattat ggcccaagat agccctgctg
     1981 cccttgccag cctggccaat ctgctggacc gggggcatga cttgcgggcg ttctgcgcgg
     2041 aaatcgtgga acacatccgc aatttgctcg tggcggcagt cgttcccggt acggtcgaac
     2101 tgcggagctt gattgaaatc tccgaagacg atctgaatca gctgtcgatg aatgccaaga
     2161 cactgactcc ggagcagctt caggagttgc tggcaatctt catccaagcg gaagatgctt
     2221 tacgattcac caaccaccct cgttttgtga tggagaccgc tgctgttcga gccacacggt
     2281 tgttgcgtca acgagagggc atagcggcgc gcccggtaca gacagcgtcg tcatccaccc
     2341 agaaacctcc cgttgagccg gaaggacgca agagtggacc atcgactcct ccggctttgc
     2401 gccagaatgg acccgttgcc tcaagacctt cacctaaacc tacgcaaacc tctagggcgg
     2461 aaacagaagg cgggcgatcg atctcttctg aaccatctca cgatccccca actgatgtgg
     2521 cgacagccac cccagccgcc gtacctcctc tagccgtcga tccacaaccg acgttgcatt
     2581 gggaattggt ccaagaagag gttgcagcct catttccaaa tatcgcgccg tttctcgaag
     2641 ccggcaggct tgtcgggatg gaggatggta tcgtcaccat cgggtttgcc aagcaggcaa
     2701 ccgtagccag agctagattg gagaaggaag agaaccttct cgtgttatca aaattat
//
LOCUS       DATMTI010000074         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_257507, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000074 DATMTI010000000
VERSION     DATMTI010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257507"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..1900)
                     /locus_tag="VN039_01285"
     CDS             complement(323..1900)
                     /locus_tag="VN039_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HWV44653.1"
                     /translation="MNSLDRRTFLKVVGAIPFAVWLKRDAVAATGPLVRYNVLSTQGQ
                     AMLKIYADGVSKMKALFDSDPRSWTFQWYIHAVRDDRTKTTELSRIYPVPGPERTLAS
                     TVWSTCQAHFNHVNEPFFLPWHRMYVLRFERIIRKLTNQPTFTLPYWNYSAAGSTHGI
                     LPAAFRTTTSSLFVQNRIASVNAGKAIDASSPGALATTALSQCSYNPQGSVQGFCQAL
                     DSGLHGNVHVLIGDRKNMGSVPWAARDPIFWLHHCNIDRLWASWNKGGRSNPTSTTFL
                     NKTFTFADENGQQVVGKVSDVLSILRLGYAYSAYEQVPPCMQSSSTALAESEPSTAQE
                     ELEPSMAQEESAPSMAQEESAPPMAQEESTREATRVLTAPAPVPLASEPVQVQLKMPE
                     APAAVAPDTTLDRRIRKLAPSTRVYLVISKLQAAAQPDVLYEVYFAPSEEAREKPPAR
                     HRVGTINFFNAVSHGEDAQSLVNDERFISFDITHLVRRLQLEGRLKAEPSVIIVPAGE
                     PATDARPVIGSIELTFQ"
     gene            complement(1936..2328)
                     /locus_tag="VN039_01290"
     CDS             complement(1936..2328)
                     /locus_tag="VN039_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44654.1"
                     /translation="MLRPPQDLARVAPGELLRRAVASRRLLLLLCDLRAVQTPETLFH
                     VYLNLPEQADQATRGRHLLAQFNFFGAGRPGDTTMPVWQSFDVTHVVSTLAEQGVLET
                     EATLTILAARTFDSKSRPSVERIAIVQQ"
     gene            3098..>3574
                     /locus_tag="VN039_01295"
     CDS             3098..>3574
                     /locus_tag="VN039_01295"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HWV44655.1"
                     /translation="MERVVPPTELTALRGKLRDTWLLHQYRSIKRRVLRRSEGEAFLL
                     RRYARIHGKPLNLTNPQTYTEKLFWRMITWNRGNMPLRVTQLADKYAVRAYVANTVGK
                     QHLIKLLWQGNDPRAIPFDRLPDEYVIKPNHAAGQVIIVKGQADRDEIIQTVSGWLA"
BASE COUNT          795 a          952 c         1000 g          827 t
ORIGIN      
        1 aatagcgccg gtttttacca ggatcatgag atcgcaatcg ttagggaggt catatattgg
       61 gctagaaccg gtacgataga caagaccatc gctgccatct gatgaggcta tttgcaagaa
      121 gttgcaaagg agtcctcatc aagattatgt gtaatcggaa gtgagccact tggttgtagc
      181 gttgatgcat tgaaaccgta tacagccctc tccaggagtc cgggattatg aggatcctcg
      241 atctcgatag atctggcgcg atcatgtcta tgaatgattg accattaacc tcccatgcga
      301 gcttgatctc cgagtgttaa gcctactgga aagtgagctc gatcgaccca ataacaggcc
      361 tcgcgtctgt tgcgggctct cccgctggga cgatgatgac cgatggttcg gccttgagcc
      421 gcccttccaa ctgcaagcga cgtacgaggt gtgtaatgtc gaagctgatg aagcgttcgt
      481 cgtttacgag ggattgagcg tcctcaccat ggctcacggc gttaaagaaa ttaatcgttc
      541 cgactcggtg cctcgccggt ggcttttccc ttgcctcctc ggatggcgcg aagtacacct
      601 catagaggac gtccggctgc gcggctgctt gtagcttgga tatcacgagg tacacgcgtg
      661 tactcggcgc caatttacgt atccgcctgt cgagagtcgt gtcaggcgcg acggcggcag
      721 gcgcttcggg catcttcagc tggacctgta cgggctcgga cgcaagcggc acgggcgcgg
      781 gggcagtcag cacgcgcgtg gcttctcgcg tcgattcttc ctgtgccatg ggtggcgccg
      841 attcttcctg ggccatggat ggcgccgatt cttcctgggc catggatggc tccaattctt
      901 cctgggccgt ggatggttcc gattcggcaa gagccgtact gctcgactgc atacaaggtg
      961 gtacctgttc gtacgcgctg taggcgtagc ccagcctcaa aatggacagc acatcgctca
     1021 ccttcccgac cacctgctgt ccattctcgt cagcgaacgt aaatgtcttg ttaaggaacg
     1081 ttgtgctggt tgggttcgaa cgaccacctt tattccagct ggcccacagc cgatcgatgt
     1141 tgcaatggtg cagccaaaag attgggtcac gcgctgccca gggcacggac cccatgtttt
     1201 tcctgtcgcc gatgaggaca tgcacatttc cgtgtaatcc agaatcgagc gcttggcaaa
     1261 acccctggac tgagccttgc ggattgtagc tgcactggga aagggcggtt gtggcgagcg
     1321 ctccaggact ggaggcgtcg attgctttgc ctgcattaac cgatgcgatt cgattttgca
     1381 cgaatagcga actcgtcgtg gttcgaaacg cagcgggaag gatcccatgc gttgatcctg
     1441 ctgccgaata gttccagtag ggcaacgtaa atgtgggctg gttcgtgagc ttgcggatga
     1501 tcctctcaaa ccggaggacg tacatgcggt gccatggcag gaagaacggc tcattgacgt
     1561 ggttaaagtg tgcctggcag gtgctccaga cggtacttgc cagcgtccgc tctggtccgg
     1621 gcacgggata gatccggctg agctccgtcg tcttggtacg atcgtcacgc accgcatgga
     1681 tataccactg gaacgtccag ctgcgcgggt cgctatcaaa tagagctttc attttagaga
     1741 caccgtccgc atagattttt aacatcgcct gtccctgcgt gctgagcacg ttgtagcgga
     1801 ccaatggacc cgttgcggcg acggcatctc gtttcaacca tactgcaaat gggattgccc
     1861 cgacgacctt gagaaatgtt cgacggtcca aagagttcat tcgtctttcc tcctagatgt
     1921 gagcaaacaa ggacgctact gctggacaat tgcgatacgt tcgacgctcg gccgagattt
     1981 ggaatcaaac gtccgtgccg ccaagatagt cagcgtagcc tctgtctcga gcacgccctg
     2041 ctcggcgagt gtgctgacca catgagtgac atcaaagctc tgccagacgg gcattgtcgt
     2101 gtcgccgggc cgtcccgccc cgaagaagtt gaactgagca agtaaatgtc tcccgcgcgt
     2161 ggcctggtca gcctgctccg gcaggtttag gtagacgtgg aacaacgtct cgggagtctg
     2221 cacagcgcgg agatcgcata gcagcaaaag caggcgccgg cttgcaactg cccggcgtag
     2281 taattcgccg ggcgcgaccc gtgcgagatc ttgcggtgga cgcagcacgc tccggattcc
     2341 ttcagggtga agcactatcg gaccatgcga ggctgactcg agcaacgtgt tacgtgggtc
     2401 cacatctgca tgtccttccc gaccaccaag acacgcttcc gataattgag gagcggtgac
     2461 gtgtgttgtg atcgtcaaca tgaccatgat tgcgaagcgc aagatcgtct cctattcgtg
     2521 aagcgttccg acgcttcgcg ccttttctta ctttagcatc gatacgtgct gcttcttagc
     2581 ggatctttcc aatatgctca tgacaaaagg tattttcaag aggaaatggc ggttggattc
     2641 tagggttatc cctaggtcat atacggagcc tgtcgctgat ataatcatcg acgcggccga
     2701 acaagcacca aaaagcctca tcccagtatc caattcagac ggaagggcgg caagcggtct
     2761 ataaccgaac ggattttcga tacaaggcac ataccctggt tcagtagtga tgttctgacc
     2821 gccacccgca cgaccagcat cgttcgctaa ctgcgtctgt aattttcacc tcgatccaaa
     2881 gtaatcaatg gtggaaagtg tcttgtctaa gccataacta ctactcaaga taagagtagg
     2941 tcagccggtt ttcttgagta gcttgactgt ggttaaaata cttagctacg tatatttcat
     3001 catttcgtag gatgtacatt gccagtcacg acgttttaaa atgcgatcca atgcaaaata
     3061 tatgagaagc gcagtcgaag cccgctagag gctcataatg gaacgcgtgg tcccacctac
     3121 agaactcaca gcccttcgag gcaagctacg ggatacctgg ctcctccatc aataccgaag
     3181 catcaaacgt cgggtgctgc gacggtccga aggcgaggcc ttcctattgc gtagatatgc
     3241 tcgcattcat ggcaaaccgc tcaacctgac gaatccccag acctatacag agaagctgtt
     3301 ctggcgcatg attacctgga atcgaggaaa catgcccctt cgggttacgc agctggcgga
     3361 taagtatgcg gtgcgagctt atgtggcgaa tactgtgggc aagcaacatt tgatcaagct
     3421 cttatggcag gggaacgacc cgcgtgcgat cccgtttgat cggctgccgg acgagtacgt
     3481 gatcaagccg aaccacgccg ctggacaagt gatcattgtc aaaggacagg cggaccgaga
     3541 cgagatcatc cagaccgtct caggttggtt ggcc
//
LOCUS       DATMTI010000075         8257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_766732, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000075 DATMTI010000000
VERSION     DATMTI010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766732"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..1474)
                     /locus_tag="VN039_01300"
     CDS             complement(365..1474)
                     /locus_tag="VN039_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44656.1"
                     /translation="MTMTEPATIIKGTCFALFAYDVGLSINLTDAERRIIAGTERGRL
                     RHKTRAPQYFEYRPAPLRLVQEGSVFDVSQYQASPTVEGMVYDFGAVTITYRFPIDGP
                     FARLLDLSESLYENEQLLAESRTRVEQLLQVLSPAVERPSIAQEVEDYIIFSIESHMP
                     ERPPLWTSNETDLAHILRAERAPFSEQEVADAVACRISFGQEDAAVIDWHSTALFGRE
                     MDDVRAVLEFANVELLEMRILDEQLDRALDEGYEALSRKPRLLSLPGSHEKDMTHIAQ
                     LQVDSALIFERVTNTLKLLGDQYLARVYRLVSQRFHLEAWDASILRKLQTLESIYGKM
                     SDRAGTRRMEVLEWIIIILITLSIALPFLPIGAGH"
     gene            complement(1534..3264)
                     /locus_tag="VN039_01305"
     CDS             complement(1534..3264)
                     /locus_tag="VN039_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV44657.1"
                     /translation="MVIFKRFLPFVRPHLARLILAGLLVSGVALINLALIRLAGTLWD
                     IITIRHDADQMTRSIGLFLGLVILQGLCSMGHSYLTAWVSQEVIADFRKHLFGHLQTL
                     TVSFFARRRTGELLSRLMTDVTVIQSIATETPIDGVKQLVTFVGGITFLLMMNWQLCL
                     LILILLPLLVLVAKVFGRRLKSLSTSIQDHTAALSTLVEEVISGIRIVKSFVQTQREQ
                     ARFADQVDQTLHLTLRRAGIMAVFIPVISLLTFSSATAVLWYGGKQVIDGVVTPGDLF
                     AFVLFAGILIGPFSSAARVFAQIKEAQGATQRVFEILDARPDICDRSDAQTLVTVDGH
                     VRMEGVNFSYDTRHPVLSNLSFEAMPGELVAFVGPTGAGKTTVINLLHRFYDPTEGRI
                     TIDGIDLRHVTLESWYRQIALVPQETILFGGTILDNIRYGNMAANDASVLEASKAAHA
                     HDFITSLPDEYQTLVGEKGVNLSGGQRQRIAIARAILKNPRILLLDEATSSLDTESER
                     LVQEALQRLMEGRTTFVVAHRLSTIQRADRILVLDKGKLVEEGNHAQLMARNGLYHYL
                     YTVRLNEPTA"
     gene            complement(3337..3555)
                     /locus_tag="VN039_01310"
     CDS             complement(3337..3555)
                     /locus_tag="VN039_01310"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44658.1"
                     /translation="MAEGRKVRIRVRTVNCTYVGDFLVPPMRNRVSDAINEEARLFIS
                     LTDVVIDDKDRSDFVAINKNLIESIAQL"
     gene            3830..4291
                     /locus_tag="VN039_01315"
     CDS             3830..4291
                     /locus_tag="VN039_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44659.1"
                     /translation="MLTMVAVGCPSKTIRYPAEHERLLRIDQALESLRSAYERKDRSG
                     FQSMLLPQDQLDELRRQVDLDLEAFSAISLEFKIERVVIEKDDIDVFIHWQGTWKRDA
                     NDMGIRQRGHARLQWVGTHSILLRSAQGDLPFGMKTKQMLSEPPSPSSQPR"
     gene            4294..5877
                     /gene="nadB"
                     /locus_tag="VN039_01320"
     CDS             4294..5877
                     /gene="nadB"
                     /locus_tag="VN039_01320"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HWV44660.1"
                     /translation="MPRNTLGADFLVIGSGVAGLRAALNLCRVGRVIVLTKGHPLQSN
                     SIFAQGGVAVALSEEDDVAIHLTDTVKAGHGLCRREAVRVLVEEGPDRIQELIRWGAK
                     FDKTGGKFAFAREAAHSRSRILRARGDATGNEMVRVLMAQAARQKRIVRLDYHFTVDL
                     VVEEGRCCGAVVLDEHSGEHFIIPAKAVVLSTGGAGQIFARTTNPPNATGDGMAMAFR
                     AGAELHDMEFVQFHPTSLYLPSSPPFLLSEAMRGEGGQLRNNRGETFMQRYHPLGVLA
                     PRDIVARAIWAEMAATRARHVYLDVTHLGSNFVKRRFPTIYATCLRHDIDITEEWIPV
                     SPSAHYMMGGVATDIDGATTLPGLFAAGEVACSGVHGANRLASNSLLEGLVFGMRAGV
                     AAVAWTSRRSMPDLAHHVERLRRGRLERLEDAEKVRSSLRRTMWGQVGLVRSRESLVR
                     ATAQLARWERMVSRSFAGRADLEVKNMVQVAHCVAEAALWRENSVGAHYRSDFPGSKR
                     PGWKQHSQVCLGIRTYRPG"
     gene            complement(5862..6563)
                     /locus_tag="VN039_01325"
     CDS             complement(5862..6563)
                     /locus_tag="VN039_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWV44661.1"
                     /translation="MPKQNKLHRPSIRRAGMAALVALSGSFLLTVPSDSQAEIYQYVD
                     AKGTISLTNVPSDARYRRIDLHPNRLHPVLSEQELEPVISRFSRQHQLHPALIRAIIK
                     AESDFDPRAVSRAGAIGLMQLMPQTAVKLEVRDLYDPEDNIGGGTKYLRQLLDRFRGN
                     LPLALAAYNAGERVVDRYRTLPPIDETRQYVRKVLRYYRLFLARDLGVTGHIIPSSEY
                     AMPRPASVSSNQPGR"
     gene            complement(6682..7554)
                     /locus_tag="VN039_01330"
     CDS             complement(6682..7554)
                     /locus_tag="VN039_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWV44662.1"
                     /translation="MPMWIYLNNKFVTDEEAVVSVFDHGFLYGDGVYETIRSYGPRIF
                     MRDEHLSRLFRSAAEIGLTIPIPMKNWEDILHETMIRNEIGTDLQDAYLRITVSRGTG
                     DIGLDPALCSSPTVVVMAKLLVPPASHLYETGVNVIVASTKRNLPSALSPQIKTTNFL
                     NNIQAKREAIAAGAFDSILLNWEQHLTECTISNLFFVMDGTLRTPALECGLLDGITRT
                     IVIRLAGELNIHVEEGRYTVDQLYRADECFLTNTSMEIMPVTSVDRRPVGDGKPGPLT
                     RKLRAQFVAARGRF"
     gene            complement(7558..>8257)
                     /locus_tag="VN039_01335"
     CDS             complement(7558..>8257)
                     /locus_tag="VN039_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HWV44663.1"
                     /translation="GDLQTDLSVYSRLRSLNPSPFSGVLRFDALRLISSSPERLVRLE
                     RRRADTRPIAGTRPRGRSAFDDRQLIEELHTNEKERAEHVMLVDLERNDLGRVCRFGS
                     IHVDELMTVEQYSHVNHLVSHVAGTLEDHATGFDLLKAMFPGGTITGVPKIRCMEIIE
                     ELEPVRRGPYTGSMGYLSWSGDLDFNILIRTLVMRNSVGYLQVGAGIVADSDPTREYE
                     ETIHKAQAFLSAFS"
BASE COUNT         1803 a         2210 c         2425 g         1819 t
ORIGIN      
        1 tcgccgatat tcgcgctggt cgcgaggtta ggatgtggct aacaccatct gcactggaga
       61 tcgatcactc gaattcctcc catccactca accggctgcc gaagaccagc gtacactggt
      121 cgccacggac atacccgatt cgacccataa cgcgacctcg gattgcaagc ctgccgtagc
      181 ggcgattggt gaaatcgtca aagtcagaaa aaggcaagtt gtgcaaaaaa ccgatcaaag
      241 gacaaagggt tcaaccacct tcatgtttca cgaagatcgt tttgcatggg ttccgagttt
      301 tctccacgat atcgaggtga gatcggaaca ttcagtctcc taccaggtca gaggacgcgg
      361 acgcttagtg gcctgcccct atcgggagaa acgggagagc gatggagagt gtgatcagga
      421 tgatgatgat ccactcaagc acctccatcc gtctggtgcc ggctctgtcc gacatcttgc
      481 cgtagatact ttccaatgtc tgaagtttgc gcaggatgct cgcgtcccag gcctccaaat
      541 gaaagcgctg cgagaccagc cgatagacac gtgcaagata ctggtcgccc agcagcttta
      601 aggtgttggt gacgcgttcg aaaatcaaag cgctgtcgac ctgaagctgt gcaatgtgtg
      661 tcatgtcttt ttcatgggag cccggcagcg acaggaggcg cggttttcga gacaacgctt
      721 cgtagccctc gtccagtgca cgatcgagct gttcgtcgag tatccgcatc tccaacaatt
      781 caacgttggc gaattctaaa accgcacgga cgtcgtccat ctccctgccg aacagcgcgg
      841 ttgagtgcca gtcgatgacc gcagcgtcct cctgcccgaa cgaaatccgg caagcaacag
      901 cgtcggccac ttcttgttct gaaaacggcg cccgctctgc tcgcagaata tgagccaaat
      961 cggtttcgtt gctcgtccac aacggtggcc tctccggcat gtgagactca atcgagaaaa
     1021 taatgtaatc ctctacctcc tgtgcaatgg acggccgctc gaccgcggga cttagcactt
     1081 ggagcagctg ctccacgcgg gtgcgcgact cggctaaaag ttgttcattc tcgtagagcg
     1141 actcgctgag gtccagcagt cgagcgaatg gtccgtcgat cgggaagcga tacgtgatag
     1201 tgaccgcccc gaaatcatat accatccctt cgacagtcgg actggcctga tactgactga
     1261 cgtcaaacac acttccttcc tgaacgagcc gcaagggagc cggtcgatac tcgaaatact
     1321 gaggggctct ggtcttatgg cgcagccgtc cccgttctgt ccctgcgatg atccgacgct
     1381 ccgcatcagt gaggttgatg gagaggccaa catcgtatgc gaaaagggcg aagcaggtcc
     1441 ctttgatgat ggtggcaggt tccgtcatgg tcattcctcg cacgccatca ttctaacagt
     1501 ttcatatcag ggggagattg tctggagaac gccttacgcg gtcggctcgt ttaagcggac
     1561 ggtatacaga tagtggtaca gcccgttgcg ggccattaac tgggcatggt tgccctcttc
     1621 aaccagtttg cctttgtcta aaaccagaat tcggtcggca cgttgaatgg tcgacagtcg
     1681 gtgggcgacg acgaaggtcg tgcgaccttc catgaggcgt tgaagggctt cttgaaccag
     1741 ccgttctgat tcggtgtcca gcgacgaggt cgcttcgtcc agtaacaaaa tgcgaggatt
     1801 cttcaagatc gcccgggcaa tcgcgattcg ttggcgttgt ccgccggaca ggttgacccc
     1861 cttctcgccc accaaggttt gatactcgtc cggcaagctc gtgatgaagt catgagcatg
     1921 ggcggctttg ctcgcttcta ataccgaggc atcattcgct gccatattgc cgtagcgaat
     1981 attgtcgagg atggtgcccc caaaaagaat cgtttcttgt ggaacgagag caatctgtcg
     2041 gtaccaactc tcaagggtca catggcgcaa gtctatgccg tcgatcgtga tgcgaccttc
     2101 cgtcggatcg tagaagcggt gcagcaggtt gatcacggtg gtcttgccgg cgcccgtcgg
     2161 tcctacgaag gcgaccagtt cgcccggcat ggcctcgaac gacaggttcg acaacaccgg
     2221 atgacgagta tcgtagctga agttgacgcc ttccatccgc acatggccat cgacggtcac
     2281 cagtgtctgg gcatcggatc gatcgcaaat gtccggccga gcatccagta tttcaaagac
     2341 acgctgcgtc gctccctggg cctctttgat ctgcgcaaag acgcgcgctg ccgaactgaa
     2401 ggggccgatc aagatgccgg cgaacaagac aaaggcgaac agatcgcccg gcgtgacgac
     2461 cccgtcgatg acttgtttgc ctccgtacca cagcaccgcc gttgccgatg agaacgtcag
     2521 gaggctgatt accgggatga agacggccat gattccggct cgacggaggg tgagatggag
     2581 cgtttgatcg acttgatccg caaaacgagc ctgttcccgt tgcgtctgga cgaacgattt
     2641 gacgatgcga atgccggaaa tcacttcttc gacgagggtg ctgagtgcgg cggtgtggtc
     2701 ttgaatcgaa gtggagagag atttcaatct gcggccgaat accttggcga ccagaaccag
     2761 taacggaaga aggatcagga tcaggaggca gagctgccaa ttcatcatga gcaggaacgt
     2821 gatgccgccg acgaacgtca cgagttgttt aactccgtcg atcggcgttt cagtcgcgat
     2881 ggattggatg actgtcacat cggtcattaa tcgagagagc aattctcccg tacggcgccg
     2941 agcaaaaaag ctgactgtca gcgtctgcag gtgaccgaag agatgtttgc gaaagtcggc
     3001 gatgacttcc tgagagaccc atgcggtcag ataactatga cccatcgagc atagcccctg
     3061 taagattacc aggcccagga ataagccgat cgaccgggtc atctgatcgg catcgtgccg
     3121 aatcgtgatg atatcccaga gagtgccggc tagccggatc aaggcgagat taatgagcgc
     3181 aaccccgctc accaagagcc cggccagtat taaacgcgcc aaatgagggc gcacgaacgg
     3241 gaggaaacgt ttgaagatca ccatggtggt cgggcggtgt gattggacac ggaatcgtgt
     3301 ttccggctta cgtcgactgg ggtggggacg aaacgactag agttgagcga tcgactcgat
     3361 caggttctta ttgatcgcga cgaaatccga ccggtcctta tcgtcgatca cgacatcggt
     3421 caagctgatg aagagacgtg cttcttcatt gatcgcatcg gagacacgat tccgcatcgg
     3481 agggaccagg aagtctccca cgtaggtaca attgaccgtc cgtacgcgga tacggacttt
     3541 cctcccctcg gccacggttc cctcctcacg ccacgccggc tgagtttagc tttttcggcg
     3601 aagaaatttc tgacgttggc gcagcttctt gtacttatgc ttgcgcatct tctttcgtcg
     3661 ttttttcaag acgctcgaca tgtttagtat ctcctaagga gggccgagtc taaaagcttt
     3721 ccgctgaaaa tgcaagctca cggcgccttt tccccttatg ctatactgca cggccatgcc
     3781 tcttaagcgg attggtttgt cacagcccgc atccatcgtg tggctcctca tgctcaccat
     3841 ggtagcagtt gggtgtccgt cgaagaccat tcgatatcca gcagagcacg agcgactcct
     3901 ccgtatcgac caagcgttgg aatccttacg aagcgcctat gaacgaaagg atcggtccgg
     3961 gttccagtcc atgctgttgc cgcaggatca gttggacgaa ttgcgacggc aggtcgatct
     4021 ggacctcgaa gccttttccg ccatttccct cgagttcaag atcgaacggg tcgtgatcga
     4081 aaaagacgat atcgacgtct tcatccattg gcaagggacg tggaagagag atgccaatga
     4141 catgggtatc cggcaacggg gtcacgcacg gttgcaatgg gttggtacgc actcgattct
     4201 cttgcgtagc gcgcagggag atttgccctt tggaatgaaa acgaaacaga tgttgtcgga
     4261 acctccctca ccgtcgtccc aaccacgata acgatgccgc gaaatacgct gggagcagat
     4321 tttcttgtca tcggcagcgg agtcgccggg ctacgcgccg ccttgaacct ctgccgagtg
     4381 ggtcgcgtga tcgttctcac caagggccat cccttacaaa gcaattccat cttcgcgcaa
     4441 gggggggtcg ccgtggcttt gagcgaagag gatgatgtcg ccatccattt gacggacacc
     4501 gtcaaggccg gacacggcct gtgtcgtcgt gaagcggttc gagttttggt cgaggaaggg
     4561 ccggatcgaa ttcaagagct catccgctgg ggcgcgaagt tcgacaaaac cggtgggaaa
     4621 ttcgcctttg cccgtgaagc ggctcacagc cgcagccgaa tcctccgtgc gagaggcgat
     4681 gcgacaggaa acgaaatggt gcgggttttg atggcgcagg ccgcccgaca aaagcggatc
     4741 gtccggctgg attaccactt taccgtcgat cttgtggttg aggaaggccg atgttgcgga
     4801 gcggtggtac ttgatgaaca ttcaggagaa cacttcatca tacccgcgaa ggccgtcgtc
     4861 ctatcgacag ggggagcagg acagattttt gcccgcacca caaatccacc caatgcgacc
     4921 ggcgacggca tggccatggc atttcgtgcg ggagcggaac tccatgacat ggaattcgtt
     4981 cagtttcacc cgacgtcgct gtatctgccg tcgagcccgc cctttttgtt gtcggaagcc
     5041 atgcgaggcg agggaggtca gctgcgcaac aatagaggag agacgttcat gcaacggtat
     5101 cacccgctcg gcgtcttggc tccgcgggat atcgtcgcgc gggctatctg ggcggagatg
     5161 gcggcgacgc gtgcgcggca tgtctacctt gatgtgactc acttgggatc gaattttgtc
     5221 aaacggcgat ttccgacgat ctatgcgact tgtctacgcc atgatatcga tatcacggaa
     5281 gaatggatcc cggtctctcc gagcgctcat tacatgatgg gaggggttgc gaccgacatc
     5341 gacggggcca cgacattgcc gggtcttttt gcggcgggcg aggtggcttg cagcggtgtg
     5401 cacggtgcga acagactggc gagcaactca ttgctggagg gattggtgtt tggaatgcgg
     5461 gccggcgttg ccgccgtagc ttggacctct cgtcgttcga tgcccgattt ggctcaccat
     5521 gtggagcgtt tgaggcgcgg tcggttggaa cgattggagg atgctgaaaa agtacgcagc
     5581 tctcttcgac ggactatgtg gggacaggtg gggcttgtcc gttctcggga gtcgcttgtt
     5641 cgcgccacgg ctcagcttgc gcggtgggaa cggatggtgt ccagatcctt tgcggggaga
     5701 gccgatctag aggtcaagaa catggttcaa gtggcgcatt gcgtggcgga agccgcactc
     5761 tggagggaaa acagcgtcgg tgcccattat cgatctgatt ttccgggatc caaacgtccg
     5821 ggctggaaac agcatagtca agtgtgtttg gggattcgca cttaccggcc aggctgattt
     5881 gaagacaccg aagcgggtcg tggcatggca tactccgaag acgggatgat gtggccggtc
     5941 acgccaaggt cacgggcgag gaagagtcga taataccgca agaccttgcg gacatattgg
     6001 cgggtttcgt cgatcggcgg aagagttcgg taacgatcga caacacgctc cccggcgtta
     6061 taggcagcga gcgccaacgg aaggttgccg cggaatcggt cgagcaactg gcgtaagtac
     6121 ttcgttcctc ctccgatatt atcctcggga tcgtagaggt cacgaacctc cagtttcact
     6181 gccgtttgcg gcatcaattg catcaatccg atggctcccg ctcgtgatac cgcgcgaggg
     6241 tcgaaatccg attcggcctt gatgatggcg cggataagcg cagggtggag ttgatgttgc
     6301 cgtgagaatc gactgatcac cggttccaac tcctgctccg agagaacggg atgcagccga
     6361 ttcgggtgga gatcgatccg gcggtaccga gcgtcagagg gaacgttggt gagcgatatc
     6421 gtccctttgg catcgacata ttggtaaatc tctgcctgac tatcgcttgg aacagtcagc
     6481 aggaagctgc cgctcaatgc gacgagggcg gccatgccgg cccgcctgat agagggacga
     6541 tgcagtttgt tctgtttcgg catggtcaaa cgatagcagt ctgtcactgt ctgtcaagga
     6601 aatgcagatc ttgtgccttg ggagatcagt agaagctgga ggaaaatgga aggatggggt
     6661 cttgcaggcg ggaggcccat actaaaatcg gcctcgcgcc gctacgaatt gcgctcttag
     6721 cttccgggta agcggacccg gtttcccatc gccgacaggt ctgcgatcga cggaggtcac
     6781 cggcatgatt tccatgctgg tgttcgtgag aaagcattcg tcggcccggt acaactggtc
     6841 gacggtatag cgaccttctt cgacgtgtat gttgagttcc ccggccaacc gaatcacgat
     6901 ggttctggtg atgccgtcga gtaagccgca ttccagggca ggcgttcgca gcgtgccgtc
     6961 catcacaaag aacagattgc tgatggtgca ttcggtcagg tgctgttccc aattgagaag
     7021 gatactgtcg aatgcgcctg ctgcgatcgc ttcgcgtttc gcctggatat tgttcaagaa
     7081 gtttgtagtc ttgatctgtg gcgacaaggc gctcggtaaa ttccgtttcg tggacgcaac
     7141 gattacgttg acgcccgttt catagagatg ggacgccgga ggcacgagaa gtttggccat
     7201 cacgacgacg gtcggcgacg aacagagtgc cgggtccagg ccgatgtctc cggttcctcg
     7261 agaaaccgtg atcctcagat aggcgtcctg cagatcagtc ccaatttcgt tgcgtatcat
     7321 cgtctcatgc agaatgtctt cccagttttt catcggaatc gggatcgtca ggccgatttc
     7381 cgccgcggaa cggaacagcc gtgataaatg ttcgtctcgc atgaagatgc gggggccgta
     7441 cgagcgaatc gtttcataga ccccatcgcc gtagagaaat ccatggtcga agaccgagac
     7501 caccgcctct tcgtctgtga cgaacttatt attcaggtat atccacatgg gcatgggcta
     7561 tgaaaaggcg ctcaagaaag cctgggcctt atggatcgtt tcctcatatt cgcgcgtcgg
     7621 gtctgaatcc gccacaattc ctgctccgac ctgaagatag cccacggagt tcctcataac
     7681 cagcgtgcgt atcaggatat tgaagtcaag gtctccgctc cagctgagat agcccatcga
     7741 gccggtgtac ggaccgcggc gtacgggctc caactcctcg atgatctcca tgcatcggat
     7801 tttgggtaca ccggtaatcg ttccaccagg aaacatggct ttcaagaggt cgaaacccgt
     7861 cgcgtgatcc tcgagagtgc cggccacatg cgagaccagg tggttgacat gagaatattg
     7921 ctccaccgtc atcaattcat ccacatggat gcttccgaaa cggcagactc gaccgaggtc
     7981 gttccgctcg aggtcgacca acatgacatg ttccgcacgt tccttttcgt ttgtgtgtaa
     8041 ttcttcgatc agctgccgat cgtcgaaggc gcttctccct cggggtctgg tccccgcgat
     8101 cggcctcgta tcggctcgac gcctttccag ccgaacgagc cgttccggtg aggaactgat
     8161 gagacgaagt gcatcgaacc ggagtactcc tgagaaaggc gaagggttca acgagcgcaa
     8221 gcgactatag acagacagat cggtttgaag gtccccg
//
LOCUS       DATMTI010000076         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_384792, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000076 DATMTI010000000
VERSION     DATMTI010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384792"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1865
                     /locus_tag="VN039_01340"
     CDS             156..1865
                     /locus_tag="VN039_01340"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWV44664.1"
                     /translation="MKKLSSGLLALVVLCSLCVAATGVYAQEVGASSVEFPYTGNRTA
                     VWVVAQLHILFAAFILGAPIFVVISEWLGYRKQDPRYDRLAREITKVTVILFSMTAVT
                     GGLFIFVLLAAYPQFTTSFINQFYMVFAVMYPALFIAETILMYAYLYTWDVWKGEKKG
                     RHIALGVLLNLVCLLILFVINGPTSFMNTPPKAEGVSPQDFIAAATLWDKIANQSWFP
                     LSLHRIDGNVAFGGFITGMIAAYMYMGSKTQEERAYYDWMGFAAIWIAVGGSLLQPFT
                     GLLLAYEMCDYDFSFCPYMMADQLSMFFEMQGMMIGLLFLAINYYSWLSVKRIEGADN
                     VRMSILAPIVLVVLLPVTMWVMDKYWIPDPMSLAFLLPLTLAPFLLGRFVPKTVSART
                     VIKIGFIVMLVSDAVWLTPGGFVATGTDMPDELKLPEGWDYLSSMPAKLSAILTLVFV
                     TVVNYVLYNRTIKQGTILWGKIDFVSQFVLIFLAFISTWIMGLMGAVRSLLKKYFHTY
                     SLVSDFSAESFTPTLSYSAWWITAITVFFIAIVSVAALVALRPSVSRVREPEGSPVPV
                     AAK"
     gene            1905..2723
                     /locus_tag="VN039_01345"
     CDS             1905..2723
                     /locus_tag="VN039_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWV44665.1"
                     /translation="MGNIFKKLGIGLVVGSVLAGITRSQDVPLDFQLLFFIFSLIGAI
                     VFMLLDARPLSTMSGGKSLFAVIAFWILMVTVCVAGASLLPQFDPDDEKEKIDKLLKS
                     QRAASQQGKAEELIARAKALDEQVKALEQRLKGLGGGQAASASQAPPAGDKPASTAGA
                     SSGGGGDFMKVGEEQWQLQECYNCHKLRGEGGKKRGPELDNIGSLLTVDEIQQKIADP
                     KSFMAEGYEKEYQKGIMPNKFKDLMEPKEMQALAAWLGTFKNTSVNTPKPIKKS"
     gene            2723..>3373
                     /locus_tag="VN039_01350"
     CDS             2723..>3373
                     /locus_tag="VN039_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44666.1"
                     /translation="MLQPKLKSKVRCIDLDIGEVTKVVLDPLSHEISHIVVSMNGSGE
                     RQVAMGHVQAVTEDFVQLRAPSTDILALPPFKREDYVTTHEVEISHLEDNIHVTPGEV
                     LVPLPDLEKNVKRRTFFMNFTHVIGFLIGLPIAYPIIRYLMKPMYADFDNHWLNVGNV
                     GKIKQDDVGVQFKYKKKVKEVYMPESEIDKNVWILRATPELLEKVYKDKDMEFRDAK"
BASE COUNT          715 a          870 c          966 g          822 t
ORIGIN      
        1 aggattgggg gaatctgcga tactctgccg gtcggtaaga tcattatgag acggtgctac
       61 ggcaaatcgc aaggagcctg gcctgggcac ctgtcataca agtgaaagca agatccgtag
      121 actaggttct ataaagtagg gggcatcgag gcatcgtgaa aaaactgtcg agcggtctct
      181 tggctctcgt tgtattgtgc tcgctctgtg tggctgccac cggtgtctac gctcaagagg
      241 ttggagcgtc ttcggtggag tttccgtaca cgggcaatcg gacggctgta tgggtcgtcg
      301 ctcaactcca tatcttattc gccgcgttca tcctcggcgc accgatcttc gtcgtcattt
      361 cagaatggtt ggggtaccgt aagcaggacc cccgctatga tcgtctggct agagagatca
      421 ccaaggtgac ggtcattctc ttcagtatga cggcggtgac gggcggcttg tttattttcg
      481 tccttcttgc cgcctacccg cagttcacga cctcgttcat caatcagttt tatatggtct
      541 tcgccgtcat gtatccggcg ctgttcatcg ccgagaccat cctcatgtat gcctacctct
      601 atacctggga tgtctggaag ggtgaaaaga aagggcgtca catcgctctt ggcgtgctct
      661 tgaatcttgt ctgtctgctc atcttgtttg tcatcaacgg ccccacgtcg ttcatgaaca
      721 caccgccgaa ggctgaaggg gtttcgccgc aggatttcat cgcggcggca accttatggg
      781 ataagattgc gaaccaaagt tggttccctc tgagtctcca tcgaatcgac gggaacgtgg
      841 cattcggtgg gtttatcacc gggatgatcg ccgcctacat gtacatgggg tcgaagacgc
      901 aggaggagcg ggcctactac gattggatgg gcttcgccgc catctggatc gcagtgggtg
      961 ggtcgctcct gcagccgttc accgggcttt tgttggcgta tgaaatgtgc gattacgact
     1021 tttcattctg cccgtacatg atggccgatc agctctccat gttttttgaa atgcagggca
     1081 tgatgatcgg gttgttgttc ttggccatca attattacag ttggctcagc gtcaaacgaa
     1141 tcgaaggggc cgacaatgtt cggatgtcga tcctggctcc cattgtcctc gtggtcctcc
     1201 ttcctgtcac catgtgggtg atggataagt attggattcc cgatccgatg tcgttggcgt
     1261 tcttgttgcc cctgaccttg gcgccctttt tattgggccg gtttgttccc aagaccgtct
     1321 ccgcgcggac cgtcatcaag atcggcttta tcgtcatgct cgtcagcgac gccgtttggc
     1381 tgacccccgg tggattcgtg gccaccggca ccgatatgcc cgatgagctg aaactgccgg
     1441 aagggtggga ttatttgtcg tcgatgccgg caaagctctc cgcgatcttg acgttggtgt
     1501 tcgtgacggt cgtcaattac gtcctgtata accgaaccat caagcagggc acgatcctct
     1561 ggggaaaaat tgatttcgta tcccaattcg ttctgatttt cttggcgttc atctctacat
     1621 ggatcatggg attgatgggc gccgtccggt cgctgctcaa aaaatatttt cacacctata
     1681 gtctggtctc agacttcagc gcggagtcct ttactccgac gctctcgtat tcggcgtggt
     1741 ggatcacggc gattaccgta ttctttatcg ccatcgtcag cgtggctgcg ctggtagctc
     1801 tgcggccttc tgtttcgagg gtacgagaac ctgagggaag tccggtcccg gtcgcggcca
     1861 agtaatcaca gtgtggagcg cctactgagg caacgagaat tcgcatggga aatatattca
     1921 aaaagctggg cattgggttg gtggtcggta gtgtgttggc cggtatcacg cgctcacagg
     1981 acgtgccgct cgactttcaa ctgctgttct ttatcttctc gctgatcggg gcgatcgtct
     2041 tcatgctgct tgacgcgcgg ccactcagta cgatgagcgg tgggaagtct ctctttgccg
     2101 tgatcgcatt ctggatcctc atggtgacgg tttgtgtcgc cggggcgtcg ctcttgcctc
     2161 agtttgatcc cgacgacgag aaggagaaga tcgacaaact tttgaagtct caacgggccg
     2221 cctctcaaca ggggaaggct gaagaactga ttgcccgcgc caaggctctt gatgaacagg
     2281 tcaaagccct cgaacagcga ctcaagggat tggggggagg tcaggctgcg tccgcatccc
     2341 aggcgcctcc ggccggagat aagcccgcct cgacagctgg cgcgtcctcc ggagggggcg
     2401 gcgatttcat gaaggtgggc gaggagcagt ggcaactgca agaatgttac aactgccaca
     2461 agctgagggg tgagggcgga aagaaacgag gccccgaatt ggataatatc ggctcgttgc
     2521 tcacggtcga cgaaatccag cagaaaattg cggatcctaa gagctttatg gctgagggct
     2581 atgagaagga ataccagaag ggtatcatgc ccaacaagtt taaagatctg atggagccca
     2641 aggaaatgca ggctctcgcc gcttggttag gtaccttcaa gaacacctcg gtgaataccc
     2701 cgaagccgat caagaaaagc tgatgttgca accgaaactg aaatccaagg tccggtgcat
     2761 cgacctcgac atcggcgaag tcacgaaggt cgtgctcgat ccgctttccc acgagatcag
     2821 ccatatcgtg gtgtccatga acgggagcgg cgagcgacag gtcgccatgg gccatgtcca
     2881 ggccgtgacg gaggacttcg tgcagttgcg cgcgccgtca acggatatcc tcgccttgcc
     2941 tcccttcaag cgagaagatt atgtcaccac gcatgaagtg gagatctcgc acctcgagga
     3001 caacatccat gtgacgccgg gtgaagtgtt ggtgcctttg cccgacctcg aaaaaaacgt
     3061 caagcgccgc acgtttttca tgaatttcac gcatgtcatc gggttcttga tcggcctccc
     3121 catcgcgtat cccatcatcc gttatctgat gaagccgatg tatgcggatt tcgacaacca
     3181 ctggttgaac gtgggcaatg tcggcaagat caagcaggac gatgtcggcg tgcagttcaa
     3241 gtacaagaaa aaggtcaaag aagtctatat gccggagagc gaaatcgata aaaatgtctg
     3301 gatcctccga gcgactcccg agcttctcga gaaagtctac aaagacaagg acatggagtt
     3361 tcgagatgcc aag
//
LOCUS       DATMTI010000077         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_3300, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000077 DATMTI010000000
VERSION     DATMTI010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1666
                     /locus_tag="VN039_01355"
     rRNA            122..1666
                     /locus_tag="VN039_01355"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1766..1842
                     /locus_tag="VN039_01360"
     tRNA            1766..1842
                     /locus_tag="VN039_01360"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1800..1802,aa:Ile,seq:gat)
     gene            1848..1923
                     /locus_tag="VN039_01365"
     tRNA            1848..1923
                     /locus_tag="VN039_01365"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1881..1883,aa:Ala,seq:tgc)
     gene            2141..>2255
                     /locus_tag="VN039_01370"
     rRNA            2141..>2255
                     /locus_tag="VN039_01370"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          566 a          518 c          715 g          456 t
ORIGIN      
        1 gtggaagctt tagctgttca ccaatgcaag cgtctaacct aagagtcaat tcaaaagctt
       61 gacgttttac ggttaggaca acgcttcaga aagatagatc atctacggat ggtcgaaaac
      121 ttaagtgaag agtttgatcc tggctcagat tgaacgatgg cggcatgcct aacacatgca
      181 agtcgaacgg cagcacagtc gtagcaatac gatgggtggc gagtggcgga cgggtgagga
      241 atacatcggg acctgcccag acgtggggga taacgtaggg aaacttacgc taataccgca
      301 tacgtcctac gggagaaagc gggggatcga aagacctcgc gcggttggat ggaccgatgt
      361 gcgattagct tgttggtgag gtaatggctc accaaggcga cgatcgctag ctggtctgag
      421 aggatgatca gccacactgg aactgagaca cggtccagac tcctacggga ggcagcagtg
      481 gggaatattg gacaatgggc gcaagcctga tccagcaatg ccgcgtgtgt gaagaaggcc
      541 ttcgggttgt aaagcacttt tatcaggaac gaaataccac gggttaatac cctgtggggc
      601 tgacggtacc tgaggaataa gcaccggcta acttcgtgcc agcagccgcg gtaatacgaa
      661 gggtgcaagc gttaatcgga attactgggc gtaaagggtg cgtaggcggt tcgttaagtc
      721 tgttgtgaaa tccccgggct caacctggga atggcaatgg atactggcgg gctagagtgt
      781 gtcagaggat ggtggaattc ccggtgtagc ggtgaaatgc gtagagatcg ggaggaacat
      841 cagtggcgaa ggcggccatc tgggacaaca ctgacgctga agcacgaaag cgtggggagc
      901 aaacaggatt agataccctg gtagtccacg ccctaaacga tgcgaactgg atgttggtct
      961 caactcggag atcagtgtcg aagctaacgc gttaagttcg ccgcctgggg agtacggtcg
     1021 caagactgaa actcaaagga attgacgggg gcccgcacaa gcggtggagt atgtggttta
     1081 attcgatgca acgcgaagaa ccttacctgg ccttgacatg tccggaatcc tgcagagatg
     1141 cgggagtgcc ttcgggaatc ggaacacagg tgctgcatgg ctgtcgtcag ctcgtgtcgt
     1201 gagatgttgg gttaagtccc gcaacgagcg caacccttgt ccttagttgc cagcgagtaa
     1261 agtcgggaac agtaaggaga ctgccggtga caaaccggag gtaggtgggg atgacgtcaa
     1321 gtcctcatgg cccttacggc cagggctaca cacgtactac aatggtcggt acagagggtt
     1381 gcaataccgc gaggtggagc caatcccaga aagccgatcc cagtccggat cgaagtctgc
     1441 aactcgactt cgtgaagtcg gaatcgctag taatcgcgga tcagctatgc cgcggtgaat
     1501 acgttcccgg gccttgtaca caccgcccgt cacaccatgg gagtgagttg ctccagaagc
     1561 cgttagtcta accgcaaggg ggacgacgac cacggagtgg ttcatgactg gggtgaagtc
     1621 gtaacaaggt agccgtatcg gaaggtgcgg ctggatcacc tcctttcgag ataggcagcc
     1681 ttcgttgctg caagacgtcc acacaagaca cctgcacatc cgcatgccac aaggcatgaa
     1741 gggccttcgg gtccataagc tttatgggtc tgtagctcag gtggttagag cgcacccctg
     1801 ataagggtga ggccggtggt tcgagtcctc ccagacccac caccattggg gccatagctc
     1861 agctgggaga gcacctgctt tgcaagcagg gggtcgtcgg ttcgatcccg actggctcca
     1921 ccagttagac ggatgtgtag tgcacacgaa agattgattg aaaacgagac ggcatagagg
     1981 ccgttctcgt gatctttgaa aacgtaaacg agtgacaagc gttttggttc gaacgaaccg
     2041 agatgtgtcg ttgaggcaaa tatagcgaat gcgttgtttg gcgaatccca atgttcctga
     2101 ggaggaacgg gagtagcccc gaggcgactt ggggttatat ggtcaagcga ctaagcgtat
     2161 acggtggatg ccttggcagt cagaggcgat gaaggacgtg gcagcctgcg aaaagtgtcg
     2221 gggagctggc aacaagcttt gatccggcaa tgtcc
//
LOCUS       DATMTI010000078         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_724369, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000078 DATMTI010000000
VERSION     DATMTI010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724369"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..569
                     /locus_tag="VN039_01375"
     CDS             147..569
                     /locus_tag="VN039_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44667.1"
                     /translation="MSTNRFLRVQTGRVLFIGATFCTLLVLTGESVLAHGGDISETSP
                     SQSSASSPSNLSSPEAAGLQAACWALTVPYGAAKMAYAIGGGIVGGLAWAITGGNMEV
                     AKSIWIPSMTGDYIVQPQHLTGEKHLYFVGVSSEVPLS"
     gene            complement(631..1008)
                     /locus_tag="VN039_01380"
     CDS             complement(631..1008)
                     /locus_tag="VN039_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44668.1"
                     /translation="MTKSEEDLRRAVVIVAVLNLAYFGVEFTMAEIIGSVSLLADSID
                     FLEDASINFLIAVALGWTMARRAVVGKMLAGILLIRGLATLWMAWQKYSLPIPPEAIP
                     LSLTGGGALAVNVSCAFLLTKSI"
BASE COUNT          404 a          499 c          452 g          419 t
ORIGIN      
        1 acgaaccata accaaactgc tggttccata gggtggcttc ttatcctcct cgatcacgga
       61 gttcccgtcc caggaagccg aggcacgtaa gcgagcattc gtgctggtct cgcatgttgg
      121 acagaccgca ccactggaga acagccatga gtacgaatcg ctttctacgg gtgcagaccg
      181 ggcgggtgct ttttattggg gctaccttct gcacactact ggtcctgaca ggggaatctg
      241 tactggctca tggtggagac atatccgaga cgagtccatc gcaatcgagc gcgtccagcc
      301 catcgaatct atccagccct gaggcagccg ggctccaagc agcctgctgg gctctcaccg
      361 ttccctatgg tgccgcgaaa atggcctatg caatcggcgg aggcattgtg ggcggcctcg
      421 cttgggcgat aacggggggt aacatggagg tggcaaagtc catctggatt ccctcgatga
      481 caggcgacta catcgtccaa ccgcagcatc tcaccggaga gaagcacctc tacttcgtgg
      541 gcgtgtcgtc agaggtcccc ctgtcataac acctcatcac accttctctt agttttcgtg
      601 cagtctgtcc aaataatggg gtctgactcc ttaaattgat ttagttagca ggaacgcgca
      661 ggatacattg acggctaagg caccgccacc cgtgagggat agcggaatag cctccggcgg
      721 gatgggtaga gaatatttct gccatgccat ccagagtgtc gccagaccac gaattaacag
      781 gatgcccgcc aacatcttcc ccacgactgc gcggcgagcc attgtccagc ccagtgcgac
      841 ggcaatgaga aaattaattg aggcatcctc cagaaaatca atactgtcgg cgagcagcga
      901 caccgaacca atgatttccg ccatggtgaa ctcgacgcca aaatacgcca gattcagcac
      961 ggcaacgatc accaccgccc gccgcaaatc ctcttcactc ttcgtcataa ctgtctcgct
     1021 acattccatc gtgtctaaac agggtgatag catggccgcc acggtaccgc acccggaagc
     1081 taagctcttc tcacatcaaa ctacctcaag aaggagatgg gatgttcaca gtcataatct
     1141 atgtcgcggc ggccattgct gaaattgccg ggtgttttgc cttttggttt tggtacctac
     1201 cgcccaggca gagccgctga aggcgttgct tcgataccgt atcgactgaa tgccgcacat
     1261 agtggtaggc taccagccga acttaccgga cacagtatga gccattcctc aatccgtatt
     1321 ggtcttatct ttttcttgac tctccttcta ggagtgcaac tcaccggtct cacgtgtctg
     1381 gatgagtggc aaggtgcgat ctactcagca caagctgaaa tcgtggacaa ttctcagtct
     1441 cctgagggga acgtgttgga tcatgggtgt ccctgtcatt ttgtatttca gtcgaccctc
     1501 cttgccgttc ctgagaccct gtcccttcac acagatggtg taaattcaat tccctcgtta
     1561 tatgtaccga gcttcatcgt gttcctattc cgtcctcccc tcagcgtcta acacgccagc
     1621 tgtttgcaca acgtgacaag tgagcattgt ccgccatgcg ggcaatgagg aagcttcatg
     1681 cacttttgcg ttctctatcc atattgagtg cataggaact atggctcaag acgaggttgg
     1741 ggtgaggacg aaggagctac tttcatgatg gaga
//
LOCUS       DATMTI010000079         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_809438, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000079 DATMTI010000000
VERSION     DATMTI010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809438"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VN039_01385"
     CDS             <1..199
                     /locus_tag="VN039_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44669.1"
                     /translation="LRVLENAGLLRRRRYGRVHEMQLNPQPLKKAAQWVEEYRKFWEG
                     SLDRLTAYLEKTDKTAGKGKA"
     gene            321..1079
                     /locus_tag="VN039_01390"
     CDS             321..1079
                     /locus_tag="VN039_01390"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HWV44670.1"
                     /translation="MKTTELENREVVSPKEWEAACEQLLAKEKEVTRARDALAAERRR
                     MPWMAVEKRYEFEGPQGKVSLFDLFDGRRQLILYRAFFEPGVVGWPEHACVGCSMVAD
                     QVAHPAHLNARNTTLVFASRAPQNEIKRLKSRMAWELIPWYTITDDFDKDFGVDEWHG
                     TNAFIRDGERVFRTYFVNNRGDEQMGGTWNYLDITALGRQEEWEDSPKGSPKTPPYEW
                     WNWHDEYGNDEAAARVLEQVRRGREGGQAAAENR"
     gene            1149..1604
                     /locus_tag="VN039_01395"
     CDS             1149..1604
                     /locus_tag="VN039_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWV44671.1"
                     /translation="MNETKEMVEAIILERSFDAPVARVWTALTDRDQMREWYFDLKEF
                     KPQVGFEFEFVVEHEGRSYHHLCRVTDVVPEKKIACSWRYKGEPGDSLVTFELFPDGN
                     NRTRLKLTHTGTETFPRTPTYARNNFEAGWTAIIGSELKQFLEKSQAKP"
     gene            1601..2050
                     /locus_tag="VN039_01400"
     CDS             1601..2050
                     /locus_tag="VN039_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWV44672.1"
                     /translation="MKEKTAEKTSLEIVRFINVPTDRVYDAWTDPAQLRQWFGPENVR
                     TRKFTADVRVGGNYRWDLTSPEGEEMSAFGEYKELVPGKKIVFTWQWDNDEAWENRTS
                     VVTIELVESRGGTELRLKHEKLPSEESRDRHNEGWNSLLDKLEQFLV"
     gene            2124..2486
                     /locus_tag="VN039_01405"
     CDS             2124..2486
                     /locus_tag="VN039_01405"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV44673.1"
                     /translation="MNTEEVARKVVELTRKQAWYDALDLYDDDIVSVEASGMGGSPET
                     RGKEGVRGKIDWWVNSMNVHSFDVQGPFVAHDRFVVLYDADVSDKKTNQRHKMSEVGV
                     YTVKDGKIVREEFLPRVA"
     gene            complement(2607..>2901)
                     /locus_tag="VN039_01410"
     CDS             complement(2607..>2901)
                     /locus_tag="VN039_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWV44674.1"
                     /translation="MREALQELKDDLLLKIGIHEGPCLAVLLNDRQDYFGRTVNIAAR
                     VQGLATSRSIFATSGVVTDTQASKLLQSNGISASPEKRSLRGIANQIEIFEIP"
BASE COUNT          753 a          694 c          869 g          585 t
ORIGIN      
        1 tttacgggta ttggaaaatg ccggactact tcggcgccgg cgctatggac gcgtgcatga
       61 gatgcaattg aatccacagc ccttaaaaaa agccgcgcaa tgggtggagg agtatcgaaa
      121 gttctgggag ggatcgctcg atcgcttgac ggcttatctg gagaagacgg ataagaccgc
      181 cggtaaagga aaggcttgac ccatgtgtcc ggtatgcatc gcaaacattg gtcttttgac
      241 ggcgacatcg agcgggggag tagcggtcct ggcgcttaaa acattcggca gcagaaaaca
      301 cacaagggaa acacaacact atgaaaacga cagagctgga aaatcgagaa gtagtatcac
      361 caaaggagtg ggaggctgca tgcgagcagc ttctcgcaaa ggagaaggag gtgacgcggg
      421 cccgtgatgc cctggccgcg gagcgccggc gaatgccatg gatggccgtg gagaagcgct
      481 acgaatttga aggaccacaa ggcaaggtga gtcttttcga cttgtttgat ggtcgccgtc
      541 aactgatcct ttaccgggca tttttcgaac ctggcgtggt cggttggccg gaacatgcat
      601 gtgtcggctg ctccatggtg gccgatcaag ttgctcaccc tgcgcatctg aacgcccgca
      661 ataccactct cgtgttcgct tcgcgagcgc cgcagaacga gatcaaacgt ttgaagtcgc
      721 gcatggcctg ggaattgatc ccttggtaca cgataacgga tgactttgat aaagactttg
      781 gtgtggacga gtggcacggc acgaatgcct tcatccgcga cggcgagcgc gtgtttcgaa
      841 cgtacttcgt taacaatcgc ggtgacgaac aaatgggcgg aacgtggaac tatctcgaca
      901 ttactgcgct cggccgccag gaggaatggg aagattcgcc caagggttct cccaagacac
      961 cgccgtatga atggtggaat tggcacgacg agtacggcaa cgacgaagcc gcggccaggg
     1021 ttctcgaaca ggttcggcgg ggtcgtgaag ggggacaagc agctgctgag aacaggtgat
     1081 cactccggtc gcatgcgaac agatggaacg gacaataccg aaggataaca acacgaaccg
     1141 aaaacatcat gaatgaaacc aaggaaatgg ttgaagccat cattttggag cgcagtttcg
     1201 atgcaccggt cgcgcgcgtt tggacagcgc ttactgaccg cgatcaaatg cgcgagtggt
     1261 atttcgattt aaaggagttc aagccgcagg tcggatttga attcgagttt gtggtggaac
     1321 atgagggcag gagctatcat cacctctgtc gcgtgactga cgtggttccg gaaaagaaga
     1381 tcgcctgttc gtggcgttac aaaggcgaac ccggagattc tctcgttacc ttcgaactgt
     1441 ttcccgacgg aaataacagg acgcgactga agctgacgca tacaggcacg gaaacgtttc
     1501 caagaacacc cacgtatgcg cggaacaact tcgaggcagg atggactgca attatcggat
     1561 cggagctgaa acagttcctt gaaaagtcgc aagcaaaacc atgaaagaaa aaaccgcaga
     1621 gaagacgtca ctcgaaatcg tgcgattcat caacgtaccg acggaccgcg tctatgatgc
     1681 gtggaccgat ccggcgcaat tacgacagtg gttcggacca gaaaacgttc gtacccgaaa
     1741 gttcacagcg gacgtgcggg tcggcggcaa ctaccgctgg gacctgacca gtccggaagg
     1801 ggaggagatg agcgcgttcg gcgaatacaa ggagcttgtt ccgggcaaga aaattgtttt
     1861 cacatggcaa tgggataacg acgaagcctg ggagaaccgg accagcgttg tgacgatcga
     1921 acttgtcgag agccgcggag ggacagaact gcgattgaag cacgaaaaat taccgagcga
     1981 ggaatcgcgc gatcgccaca acgaaggctg gaatagtttg ctcgataaac ttgaacaatt
     2041 cctagtgtag ggcttgtctg tgagaagcca cggcgtctga cacagacgcc ctgcaatgat
     2101 tggtggttaa caatggagga acaatgaata cagaagaagt agccagaaaa gttgtggagt
     2161 taacgagaaa acaggcatgg tacgatgcgc tcgatcttta cgacgatgac attgtcagcg
     2221 tggaagcgtc cgggatgggc ggttcgccgg aaacccgcgg taaggaaggt gtccgtggca
     2281 agatcgactg gtgggtcaac agtatgaacg tgcacagctt tgatgtgcag ggacctttcg
     2341 tcgcgcacga tcgttttgtc gttttgtacg acgccgatgt ctctgacaag aaaaccaacc
     2401 agcgtcacaa gatgtccgaa gttggcgtct acacagtgaa ggacggcaag atagtgcgag
     2461 aagagttctt gccgcgcgtc gcttgagatt cacctgttcc gaagccgttt tggtttcgga
     2521 gcacaccgtg tttctgcgca ggaggggctg tgtgcatgcg atcagttcaa tccgcgcgtt
     2581 gaagcggaac ggtcctgcaa cgttcctcac ggaatttcga aaatctcgat ctggttggca
     2641 atgccacgga gcgagcgttt ctcggggctt gcggaaatgc cgttggactg caagagcttc
     2701 gacgcctgag tgtcagtaac gacaccactc gtcgcgaaaa tggaccgtga tgtcgcgagt
     2761 ccctggactc gagccgcaat gtttacagtc cgcccgaagt aatcctgccg atcgtttagc
     2821 aaaacggcaa ggcaaggtcc ctcgtgaatc ccaatcttga gcaacaaatc atctttcagt
     2881 tcctgtaatg cttctcgcat c
//
LOCUS       DATMTI010000080         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_257703, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000080 DATMTI010000000
VERSION     DATMTI010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257703"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VN039_01415"
     CDS             <1..929
                     /locus_tag="VN039_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV44675.1"
                     /translation="LGGKLIVSGVDMLLGPRERIGLLGPNGGGKTTVLKLLAGTLEPD
                     SGTITRADRLRVVTFEQHRESLDQQVTLRRAFTPAGGDAVVYQERSVHLVSWAKRFLF
                     RPEQLDLPISRLSGGEQARLLIARLMLQPADLLILDEPTNDLDIPTLDVLEDSLLEFT
                     GALVLVTHDRWLLDRISTRLLALDGMGRAEWFADYAQWEAAQVRNATEERRTQVSKER
                     SAQAKPSKRKGLSYKEQKEWDQIETKILEAEGRVAACQAATNDPSISSSATDLQERYA
                     ALHVAQTEVERLYARWAELDEKRAQVTTSTQP"
     gene            1375..1959
                     /locus_tag="VN039_01420"
     CDS             1375..1959
                     /locus_tag="VN039_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44676.1"
                     /translation="MGRYIATGPIIIGLCLCFSACARVDTILLTNDTFPPKESAEEVA
                     VLRQTPTRPHRDLAELRISDSRLSFGSLQHKILNRAAALGADAVVFAQPQTRTIRRES
                     YQPLYGSYGPWGYNSPYYGGSGYGMYGGLYGGWGPWGGVPFGSVAVPYEYDETVRILT
                     GTAIRYTDAFGPQSSIRSDEPGSQTLTLRRTGYR"
     gene            2066..2551
                     /locus_tag="VN039_01425"
     CDS             2066..2551
                     /locus_tag="VN039_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44677.1"
                     /translation="MNRHGLRVLVVSVLAVSVAACADTGQIQRELAPPDDPVIGLLSK
                     GITQLNVNINMVSKRMNDVRQASAGTDSALQELQALDLSGWELQQRQWIVQRNHLVLT
                     RDTLQRVQLSQEEKAQALAQWQQHRRQYVKAMEDLRQQRHQLESKHLEVEARLIERGL
                     Q"
BASE COUNT          609 a          760 c          794 g          574 t
ORIGIN      
        1 cgctgggagg gaaactgatt gtatcgggcg tggacatgtt gttaggcccg cgggagcgga
       61 tcggcctcct tggccccaac ggtggcggca aaacaactgt cctcaagctc ttagccggta
      121 ccctagagcc ggatagtggc accatcacgc gtgccgatcg gctgcgcgtg gtgaccttcg
      181 agcaacaccg cgagtcattg gaccagcaag tcacgttgcg acgcgccttt accccagccg
      241 gtggcgatgc cgtcgtttac caggaacggt ccgttcactt ggtctcatgg gccaagcgct
      301 tcctcttcag gccggaacag ctggatctcc cgatttcacg tctatccggt ggagagcagg
      361 cccgtctgct gattgcacgc ctcatgcttc aaccggcaga tctcttgatt ctcgacgagc
      421 cgaccaatga tttggatatt ccgacccttg atgtgctgga agacagcctg ctggaattta
      481 ccggcgcgct tgttttggtg acacacgacc ggtggctctt ggaccgaatc tccactaggc
      541 ttctggccct cgatgggatg ggccgcgccg agtggtttgc cgactatgcc caatgggaag
      601 ccgcgcaggt aagaaatgcg acggaagaac gaagaactca ggtctcaaag gagcgctccg
      661 cgcaggcgaa gccatccaaa cggaaaggcc tgtcgtacaa agaacaaaag gaatgggacc
      721 agatcgaaac gaagatctta gaagcggaag gacgcgtagc cgcctgtcaa gcggcaacta
      781 acgatccatc catctcctca tccgctactg atttacaaga acggtacgct gcacttcatg
      841 ttgcccaaac ggaagtagaa cgcctttacg cccgctgggc cgaactcgac gagaaacgtg
      901 ctcaagtgac caccagtacg cagccatagt ggcaggggca ggcaataaac cgtatgtggc
      961 cccgcgtcac cataatctga gggcttctca tagactcaag gcatctttat aaggtaggtg
     1021 acgcattgac ttatgtgtcg agccgcagaa gcgcctctcc tctgtaaatc acaaaccgtt
     1081 cgctgtggtg aagcagccgg catcaggttg aattctcaag cgcactgggc ggcgagactt
     1141 acaggtgttg ttgctgttgt tggaacatgg cttggccaag aagagtcaca cctcctctct
     1201 tcccactggt cttgcattta tttgctcaaa tgcgggaagc tggtgcggga ttcaaccatg
     1261 ttgtccaggc cggggtgaac ggtcggatgg gcgagtaagg ttgtgccctg tattttagca
     1321 gctggtcgtg ccgccgatga agggaggcgg ctcagagctg acgcaaagtt tcgcatggga
     1381 cgatacatcg cgaccggtcc gatcatcatc ggcctctgcc tgtgcttctc cgcatgcgca
     1441 agggtggata ccatcttgct caccaacgat acctttcctc caaaggagtc tgcagaggag
     1501 gtcgccgtgt tgagacagac tccgaccagg cctcatcgcg acctcgcgga gttacggatc
     1561 agcgacagcc ggctcagttt cgggagcctg caacacaaga tcttgaatcg agccgccgcc
     1621 cttggcgccg atgccgtggt gtttgcacaa ccacaaactc ggacgatacg ccgggagtcc
     1681 tatcaacccc tctatggctc gtatggcccg tggggctaca atagcccata ctacggaggt
     1741 tcgggatatg gcatgtatgg cggtctatat ggaggatggg gtccttgggg tggggttccg
     1801 ttcggcagcg tcgcggttcc gtacgagtat gatgaaaccg tgcgaatcct cacgggaacg
     1861 gcgatccggt ataccgatgc attcggcccg cagagcagta tccgatccga cgaaccaggg
     1921 tcacagaccc tcaccttgcg acgaacaggt tatagataga tggacctcct tcatggctga
     1981 agaaggatgc gaggaaaagg agatagcgag gagccaccag cgggatgtct gacggatgtc
     2041 atggctcttt cgtgagtgag gatgtatgaa cagacatgga ttaagagtgc ttgtcgtcag
     2101 tgtgcttgcc gtcagtgtcg cagcctgtgc cgacaccggt caaatacagc gcgagcttgc
     2161 ccctcccgat gatccggtca tcggcctctt gagcaaaggc atcacgcaac tgaacgtcaa
     2221 catcaatatg gtgtcgaagc gcatgaacga tgttcggcag gcgtcggctg ggaccgattc
     2281 ggccttgcaa gaactgcagg cgctggacct gtccggctgg gagcttcagc agcggcaatg
     2341 gattgtgcag cgcaaccatc tcgtcttgac gcgcgatact cttcagcggg ttcagctgag
     2401 ccaggaagag aaagcccaag cgctcgctca atggcaacag caccgccgcc agtatgtgaa
     2461 ggcaatggaa gaccttcgcc aacagcgaca ccagttggag agcaaacatc tggaggtaga
     2521 ggcccggttg atcgaacgcg gactccagtg acagtgtcac ctgcgcgagg ttcgtcatca
     2581 cgatacatgg tttagaacca ctcttcgacg ttcggcactg ccgcgctctt caaggttcac
     2641 aatacagctt ccatcattcc cgcaacctgc ttcgagagat ctaggaagtc cgccgagtcc
     2701 ctctcttttt cccgatgtca tctggcgttt tctcaat
//
LOCUS       DATMTI010000081         1990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_3428, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000081 DATMTI010000000
VERSION     DATMTI010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3428"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VN039_01430"
     CDS             complement(<1..256)
                     /locus_tag="VN039_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWV44678.1"
                     /translation="MVHVVEVDIAGRTLRLETGRVAKQADGSIWASYGDTVVLATAVA
                     AQTAKPGTDFLPLTVDYQEKAYAAGKIPGGYFKREGRPAEK"
     gene            complement(435..704)
                     /gene="rpsO"
                     /locus_tag="VN039_01435"
     CDS             complement(435..704)
                     /gene="rpsO"
                     /locus_tag="VN039_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWV44679.1"
                     /translation="MGLLKEAKGELIKQYQQHDKDSGSPEVQIAVLTTRITYLTEHFK
                     SHKKDHHSRRGLLQLVGRRRRLLDYLRGVDEARYRAVIDRLGIRK"
     gene            complement(721..1701)
                     /gene="truB"
                     /locus_tag="VN039_01440"
     CDS             complement(721..1701)
                     /gene="truB"
                     /locus_tag="VN039_01440"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWV44680.1"
                     /translation="MGRGSQALKGMDRTGVTTDQGGALEGVLIVHKEAGWTSHDVVAK
                     VRSLLRGSKVGHAGTLDPSATGVLPILVGRATRIAEYLIDWDKEYRAVLRLGETTDTQ
                     DATGRILSRVDACEVTEDALQSAITRFRGVQRQLPPMYSAVKVGGQPLYKAARAGKTV
                     EREERSIAIHQLEILAVHGRDVILRIVCSKGTYIRTLCADIGGALGVGGHLLALERRR
                     VGPLSIEQAMTIDQVLGHLTAGTLRERFISLDQLLFQLPAVVVNDEQAQRVLHGSPIF
                     PMGAGQRSAAHAAVSVRLKNEAGQLLAIGTHDSAHVGSIRIRKVLSLLSH"
     gene            complement(1694..>1990)
                     /gene="rbfA"
                     /locus_tag="VN039_01445"
     CDS             complement(1694..>1990)
                     /gene="rbfA"
                     /locus_tag="VN039_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWV44681.1"
                     /translation="VHDVTVTDVELTGDLRIAHVFVTTMETGEAERNIFAGLSKASGF
                     VRSELGRRLSLRYLPDVIFKKDVSGARGDRVMRLLEELHGESEERENQDSPNNG"
BASE COUNT          416 a          607 c          514 g          453 t
ORIGIN      
        1 ccttttcagc cggtcgacct tctcgcttga aataacctcc ggggattttt ccggccgcgt
       61 aggccttttc ttggtagtcg acggtcaacg ggaggaaatc ggtgccgggt ttggccgttt
      121 gcgcagccac ggccgtcgcc agaacgactg tgtcgccata cgaagcccaa attgatccat
      181 cagcttgctt tgcgacgcga ccggtttcga ggcggagggt ccgacctgca atgtcgactt
      241 ctacgacgtg taccatctat ggtctcctct ggttagatcc acagatgccc actgagatgc
      301 gctcgacgac aagtgacgag ttgacaaatg aacgtctgag ctgctccgtc acctgatcac
      361 acttcgcgtg agcgcatgtc ggtgttcatc tgtgggatat atggcgccgg tcaacgccgc
      421 tgttgtactg accgttactt gcgaatgccg agtcgatcga tcacggctcg gtaacgcgcc
      481 tcatccacac cacggagata gtccagcaac cgtcgtctgc gtccgaccag ttgcaacaac
      541 ccacgccgcg agtggtgatc ttttttatgg ctcttgaaat gttcggtaag atatgtaatc
      601 cgggtggtca acacagcaat ctgaacctcc ggcgatccgg aatccttgtc atgctgctga
      661 tattgcttga tgagttcacc ttttgcttct ttcagtaatc ccatacctac tccttccgca
      721 ttaatgactc aagagactca ggacctttcg aattctaatc gatcccacat gagcggaatc
      781 gtgagttcca atggccaaca actggcctgc ttcattcttc agacgtacgg aaacagccgc
      841 atgcgcagcg gaccgttggc cggctcccat cggaaaaatg ggcgagccat gcaggacgcg
      901 ctgcgcctgc tcatcattca caaccaccgc cgggagttgg aagaggagtt ggtccaacga
      961 aatgaaccgt tcacggagcg ttcccgccgt gagatggccg agaacttgat cgatcgtcat
     1021 ggcctgttcg atcgacaatg ggccgacacg gcgacgttcg agagcaagga gatgtccgcc
     1081 gacgcctaac gcccctccga tgtcggcgca caatgtgcgt atatacgtgc cttttgaaca
     1141 cacgatgcgc aggatcacgt cacgaccgtg aacggccaga atctccagct gatgaatcgc
     1201 gatcgatcgc tcttcccgtt cgaccgtttt gcctgctctg gcagctttgt agagcggttg
     1261 tccacccact ttcacggctg agtacatcgg tggcagttgc cgttgtactc ctcggaatcg
     1321 ggtgatcgcg gactgaagcg cgtcctcagt cacctcacac gcatcgaccc tgctcaaaat
     1381 ccgcccggtt gcatcctggg tgtcggtcgt ctctcccaga cgtaagacgg cacggtactc
     1441 cttatcccaa tcgatcagat attcggcgat tctcgtggcc cgcccgacaa ggatgggcaa
     1501 gacgcccgtt gcactcgggt ccagcgtgcc ggcatgaccg actttgctcc cgcgcaataa
     1561 gctccgaacc ttggccacaa catcgtgtga agtccaaccg gcctccttgt ggacaatgag
     1621 gactccctct agagcgcctc cttggtcggt tgtgacgcct gtgcgatcca tacccttaag
     1681 agcctgcgat cccctaccca ttgtttgggg agtcctggtt ctcacgctct tcagattccc
     1741 cgtgcaattc ttccaacagc cgcatgacac ggtcgccacg agctccactg acatccttct
     1801 tgaaaatcac atccggaagg tagcggagcg agagccgccg tcctaattcc gaccgcacaa
     1861 acccactggc ttttgagaga ccggcaaaga tattccgctc ggcctcgccc gtttccatag
     1921 tcgtgacaaa aacatgagcg atgcgcaaat cccctgtgag ctcgacatcc gtgaccgtca
     1981 cgtcatgaac
//
LOCUS       DATMTI010000082         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_257720, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000082 DATMTI010000000
VERSION     DATMTI010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257720"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..425)
                     /locus_tag="VN039_01450"
     CDS             complement(225..425)
                     /locus_tag="VN039_01450"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWV44682.1"
                     /translation="MRLHVIVEQDEAGYFVAEVPALPGCLSQGKTHDEAIANVKEAIE
                     GWLEVMEAKRVVDAERTVEVVV"
     gene            complement(824..2068)
                     /locus_tag="VN039_01455"
     CDS             complement(824..2068)
                     /locus_tag="VN039_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HWV44683.1"
                     /translation="MSHPPATTAPRQAAVLFILVTVLLDMLSFGIIIPVLPKLVEEFF
                     FGNTAQAAVLYGLMGTAWAFMQFFCSPIQGALSDRFGRRPVVLLSNFGLGLDYIVMAI
                     APNVAWLVAGRVISGMASSSFSTAGAYIADVTPPEQRAAAFGKIGMVFGLGFIFGPAL
                     GGWLGAIDPRLPFWGAAALSLMNACYGFFVLPESLPSDKRMPFTWARANPVGSLRLLR
                     SHHELFGLATVAFLGYLAHTVLPGVSVLYMGYRYGWGLASVGLMMAGVGLSATIVQGG
                     LICSITNRFGERRTVLIGLLCGAVGFFIYGIAPEGWIFCLGIPVMAFWGLAGPATQSL
                     MTSRVSSTEQGQLQGAIASINGVTGLIGPTLFTQTFAYFIRSDSASRTSLELPGAPFI
                     LASLMLLGAAVIAWRTTKAGRP"
     gene            complement(2320..3603)
                     /locus_tag="VN039_01460"
     CDS             complement(2320..3603)
                     /locus_tag="VN039_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV44684.1"
                     /translation="MDQEEEMSRMAERPILRRRQWLLTRDFSLVWWSQVLSQVADGVS
                     KLALLWFVYSITGSALKTTVIGLLQTLSPIILGPVIGVVVDRLPKKAILICSDLTRGL
                     LIGLIPCWVSVESFTVQSLYVLTFLYGIATAMFIPTLSSAVPLMVKKGQLTAANALLQ
                     GTTSLGIIIGPVISGLGIAFSGSQDVLCINAVTYLASAALLFPLRLSAGMATSPPESR
                     RTTAIEDLFDGIRYTLISRRTILMLIMLASVYTFGSGAFTTLFPVFGKGLLSLGPVEV
                     GYLWSWLGVGLLLVSLCLVRLTEWDLRRRVSIITWSSAVGGVALCGLVWASNITVATL
                     LVMVIGMGLGTWTPIAWGIIQEISPAHMVGRVMALYTAIATATSMVGMTFFGWLIEVS
                     SESVSLIGIGAVLLALAMSSVWFRRRVTGEHLLVG"
     gene            complement(3793..>4238)
                     /locus_tag="VN039_01465"
     CDS             complement(3793..>4238)
                     /locus_tag="VN039_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV44685.1"
                     /translation="SGLGVGMLAVSTWLAWRHQGDLHSRLRLIVRGMMVGGLAVCSLS
                     LLETPLVAAAVVVVVGGSTAVMNPVVWALLQEATPEHLMGRVLTTFSTGSMAAAMAGM
                     TGFGWIADAVGPAQSFIGLGLVLLLTAAVAIRFMRQSATAKRVTA"
BASE COUNT         1017 a         1293 c         1179 g          749 t
ORIGIN      
        1 ctgcacgttc ttaaacagaa acagacgttt cccccaccga tggacatgtt cgatcgttct
       61 tcgcttcacc ggggctttgc aaaacgggca ggacttcatg atcgcctccc ttttcttttt
      121 cgccagttcg tccacagctt cgaatttggc cgataaatcg tgatcagaat agccgtgttc
      181 tcgtgaatcg tctgaccccg aacacaaccg aaggcgttcc gccatcagac cacaacctca
      241 accgtccgtt ctgcatcgac cacgcgcttg gcctccatca cctcaagcca cccttcgatg
      301 gcttctttca cgttggcgat ggcctcgtcg tgcgttttcc cttgcgacag acaccctggc
      361 aaagcaggca cctcggcgac aaagtaacct gcctcatcct gttccacaat aacatgcaat
      421 ctcatgcctt aatctccttc ttcagaataa ctcttgagct attatcgtga agaccctcac
      481 tatggtcaag caaaccgctt gatgtattga acatcaaaat cagggtcaga ccttgagtcg
      541 tgcatgtcga gacaatactg ttaggcatga gtaatgatct gtggagtgca agaaccaaat
      601 cacggaagag accgctgatg tgaggctaaa cttacgatct ctgtcagacc ggatccccct
      661 ttgctacacg catggatgat cgctttgcaa ccgtgcggag agtccccgtg aagtttgtta
      721 cacagagacc tcaacgtatt ctacttggct gctgtccggc ctcttaccca atcatcgtgc
      781 gaatctgatt caccatcatc gagagacaac ggaaacaaca tacctatggt ctacccgctt
      841 tcgttgttcg ccacgcaatc acagcggcgc cgaggagcat gagcgaggcg aggatgaacg
      901 gtgcaccagg cagttcgagg gacgtgcggg aggcagaatc ggaccgaatg aagtaggcga
      961 aggtttgcgt gaagagggtc ggtccgatca ggccggtcac gccgttgatg ctcgcgatgg
     1021 cgccctgaag ctggccctgt tccgtactgc tgacgcggct ggtcatgagg gattgcgtcg
     1081 ccggaccggc gagtccccag aaggccatca cggggatgcc gaggcagaag atccaaccct
     1141 caggtgcgat gccgtaaatg aaaaagccga ccgccccgca cagtaacccg atcaggaccg
     1201 tcctgcgttc gccaaaccga ttagtgatgg aacagatcaa tccgccctgc acgattgtcg
     1261 cggaaagacc aacgcccgcc atcatcagtc ccacgctggc cagcccccag ccgtaacgat
     1321 agcccatgta caggaccgac acgcccggaa ggacggtatg ggcgagatag ccgaggaacg
     1381 cgaccgtcgc gaggccgaag agctcatggt gtgagcgcaa cagccgtagc gaaccgaccg
     1441 ggttggcccg ggcccaggtg aagggcattc gtttgtcaga cggcagggat tcgggcagca
     1501 cgaagaatcc gtaacaggcg ttcatgagac tgagtgccgc cgcgccccag aacggcagcc
     1561 gaggatcgat cgcaccgagc cagccaccta aggcaggacc gaagatgaag cctagcccga
     1621 agaccatgcc gatcttaccg aacgccgccg ctcgctgctc cggcggcgtc acgtcagcga
     1681 tgtaggcacc ggcggtgctg aagctggagg aggccatccc cgaaatgaca cggcccgcca
     1741 caagccaggc cacgttcggc gcgattgcca tcacgatgta gtcgagacct aagccgaaat
     1801 tggacagcag gacgaccggc cgccggccga accgatcgga cagcgcgccc tgaatcggcg
     1861 aacagaaaaa ctgcatgaac gcccaggccg ttcccatgag gccatagagc acggccgcct
     1921 gcgcggtatt accgaagaaa aattcctcga cgagcttcgg cagcaccgga atgatgatgc
     1981 cgaacgacaa catgtcgagc aagacggtga cgagaatgaa gaggaccgcg gcttggcgcg
     2041 gtgctgtcgt ggccggggga tggctcatcg ccggcgcatc ctatcagact tgtcccccac
     2101 aacgcttttc gcatggcgca cccatccgct tcatacctac cggtttcgat ccgattgatc
     2161 ttaatccaac aggccgctgt cctgtcgcta cgtcagctca aagatttgtg gattggggca
     2221 gaccttttca atacttcgaa ctgtccatcc ggcaggctcc ggcacggctt tcgaatcctg
     2281 aattcctgag actgggagaa tggctggagg aaggactctc tatccgacta aaaggtgctc
     2341 tcccgtaacg cgtcgccgga accatacgct ggacattgcg agggcgagca ggactgcacc
     2401 gataccgatc aggctcacag actcactgga tacttcgatg agccacccga agaaggtcat
     2461 gccgaccatt gaagtcgccg tggcaatagc cgtgtagagc gccatgaccc gccccaccat
     2521 gtgggcgggc gagatttcct ggataatccc ccaggcgatc ggtgtccagg tccccaaccc
     2581 cataccgatc accatcacga gcagggtcgc caccgtgatg tttgaagccc agaccagccc
     2641 gcacaaagcc accccgccga cggcgctcga ccacgtgata atcgataccc gccgccgaag
     2701 atcccattcg gtaagccgca cgagacagag cgatacgagg aggaggccta ccccgagcca
     2761 agaccagaga tatcccacct ctaccggacc gagtgaaaga agcccctttc caaacacagg
     2821 aaacagggta gtaaaggctc cgctgccgaa cgtatagacc gaggccaaca tgatcaacat
     2881 caaaattgta cgccgagaga tcaacgtata gcgaatcccg tcgaacagat cctcaatcgc
     2941 cgttgttcgg cgactctccg gcggagacgt cgccatcccg gccgatagac ggaggggaaa
     3001 caaaagggct gcggacgcca gataggtcac cgcattgata cagagcacgt cctgtgaacc
     3061 ggaaaaggca atccccagtc cgctgatgac cgggccgatg atgatgccga ggctcgttgt
     3121 accttggagg agggcattgg cggcggtcaa ctgtcccttc ttcaccatga gcggcacggc
     3181 ggaggacagc gtcgggatga acatggcggt tgcaatcccg tagagaaacg tcagcacgta
     3241 gagtgattgg accgtaaacg attctacgga tacccagcag gggattaaac cgatcaatag
     3301 acccctcgtc agatcgctgc agatcaggat ggccttcttg ggcaatcgat cgaccaccac
     3361 gccgatcacg ggtccgagga taatcgggga caatgtttgc aggagaccga tgaccgtggt
     3421 cttcagcgcc gaaccggtga tggaatagac aaaccacaag agagccagct tgctgacacc
     3481 gtcggccacc tgcgacagca cctggctcca ccacaccagg ctgaaatcgc gcgtgaggag
     3541 ccattgtctg cgacggagga taggacgttc cgccatacga gacatctctt cttcctgatc
     3601 catcacgctg atccggggtg aaggacccgc ctgacaggat gcgtatgccg gatatttccc
     3661 gcatacgtga cctcagctta ccaggttata tcggaatggg gccgacaaga aaggaggaac
     3721 ggcgggcaga atgatggggg cgccgcgcaa cctgacgcga gtctagctca cattaataaa
     3781 tacatatgtg ggctaggccg taacccgttt agccgtggcc gattgacgca taaaccggat
     3841 cgcgacggct gccgtcagta acagaaccag accaagtccg ataaagctct gcgccggacc
     3901 gacagcatcc gcaatccacc caaagccggt catgccggcc atggcggccg ccattgaccc
     3961 cgtgctgaac gttgtgagca cgcgtcccat taagtgttcc ggggtggctt cctgcagcaa
     4021 cgcccacacg acgggattca tgaccgccgt gctaccaccg acaacaacga ccaccgccgc
     4081 agcgaccagc ggtgtttcaa gcaggctgag gctgcagacc gccaggcccc ccaccatcat
     4141 tccacggacg atgaggcgca agcggctatg cagatctccc tggtgcctcc aggcgagcca
     4201 ggtggacacc gccagcatgc cgactccgag gccggacc
//
LOCUS       DATMTI010000083         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_682092, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000083 DATMTI010000000
VERSION     DATMTI010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682092"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..800
                     /locus_tag="VN039_01470"
                     /pseudo
     CDS             1..800
                     /locus_tag="VN039_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611443.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1046..2305)
                     /locus_tag="VN039_01475"
     CDS             complement(1046..2305)
                     /locus_tag="VN039_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWV44686.1"
                     /translation="MTDCDITASAPVLAAIDISKERHEVLIEVPDKKRRRRVSVLNNL
                     DEFDRLISLLRSYQRPVRVAFEATGNYHRALAYQLGSAGFEIKLISSIALARTREALH
                     NSWDKNDPKDAQVILHMMTIGAEQFYHDPLMRGTNDIQELSKTHDMISRSKTELWHRI
                     LTHYLPLYFPEADRFHRSSRSDWFFAFLELFPSPHFIAAMSKEAFIEAARDVVGRKVG
                     KERLLADIYATSARSIGLPVAPDSDAICMFRMVLAQGRSLIAHRNQIETRAVAMLKDN
                     PDYQLLCTIPGIGPINALTILAEAGDLRRFQHHRQFLKFCGMDLSTIQSGMFRGQTKL
                     SKYGNARLRRTLWMAAQVAILQRANSFRDKFERYITKDRHNPHLRRKASTAIAAKMAR
                     TVHAVVKSGEPYRPFFEGTIHSGRTLL"
     gene            2441..2725
                     /locus_tag="VN039_01480"
                     /pseudo
     CDS             2441..2725
                     /locus_tag="VN039_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          617 a          736 c          789 g          583 t
ORIGIN      
        1 tcgaagtttt cggaagcgca gatcgcattc gtgctgaagc aagcggaaga tggaacgccg
       61 atcggcgaag tctgccgcaa ggcgggaatt tcggacgcga cgttttacaa ctggcgcaaa
      121 aaatacgctg ggctgatgcc gtcggagatg aagcggctgc ggcagctcga agaggagaac
      181 gccaagctga agcggattgt cgccgacctg tcactggaca aggccatgct tcaggacgtg
      241 ctgtcaaaaa agctctgagg cctgcccgca agcgcaagct tgtcgacacg gtcaaggtgg
      301 actggaaggt ctcgatccgg cgtgcatgtt ccgttttgaa gatcgatcgg tcgctgtatg
      361 tctacaagtc caggcgcggc gagcaggccg agttgaagct gaagatcagg gacatctgcc
      421 agacgcgagt gcgttacggt tatcgtcgcg ttcatgtcct gctcaagcgc gacgggtggc
      481 ccgtcaatcc gaagcgaatc tatcggcttt acaaggagat ggacctccag ctccgcaaca
      541 aggttcccaa gcgtcgggtc aaggcgaagc tgcgagccga ccggacggaa ccgacccatt
      601 ccaaccatgt ctgggccatg gacttcgttc atgaccagtt ggccacgggt cgcaagatta
      661 gggtgctgac ggtagtcgac accttttcgc gcttctcgcc ggcagtcgat gcccgcttca
      721 actacaaagg cgaggacgtg gttcaaaccc ttgagagaat atgcagggaa gttggatatc
      781 cagcctccat acgagtcgat aacgtcctat attcggaatg catcgccgtt ttggcagcag
      841 gtcgttatcg catcggtctc ccgatacgtc tggattatga tgtaggcccg gccgatcgtc
      901 aacgaacggt ctctcccata acaaacacag agtacgcata atcgcggctc tcaccgtcct
      961 taagacgaga tccttttgcc gtcgagcgga aggcctgaac attatctacg aggtcgctga
     1021 aacactcagc gcctctacgg ccctgtcaga gaagggtcct tccgctgtgg atcgtccctt
     1081 caaagaaggg gcgatagggt tcaccacttt tgacaaccgc atgcacggtg cgagccatct
     1141 tggcggcgat tgctgttgac gccttgcgac gcaaatgggg attgtgacgg tccttggtga
     1201 tgtatcgctc gaatttgtcg cggaagctgt tggcgcgctg caaaatcgcg acttgagctg
     1261 ccatccataa ggtccgcctc aggcgagcat tgccgtactt ggacaacttc gtctggcctc
     1321 gaaacattcc tgactggatt gtcgaaagat ccatgccgca aaacttcagg aactggcgat
     1381 ggtgttgaaa acggcgcagg tctcccgcct ctgccaaaat cgtcaaagca ttgatcgggc
     1441 cgataccggg gattgtgcat aagagttggt aatcaggatt gtccttcagc atggcgaccg
     1501 cacgcgtctc gatttgatta cggtgggcga tcaggcttct gccctgggcc aaaaccatac
     1561 ggaacatgca gattgcgtcg gagtcaggtg caaccggaag gccgattgaa cgggcggacg
     1621 tggcgtaaat atctgccagc aaacgctctt tgccaacctt acgtcccacg acatccctgg
     1681 cagcttcgat gaaagcctcc ttgctcatgg ccgcgatgaa gtgcggagac ggaaacagtt
     1741 cgaggaaggc gaagaaccag tcgctgcgtg aactgcggtg aaagcgatcc gcttcaggaa
     1801 aatacaatgg caggtaatgt gtcaggatcc gatgccacag ttcggtcttc gaccgtgaga
     1861 tcatgtcgtg ggtcttcgac agctcttgaa tgtcgttggt gccgcgcatc aacgggtcat
     1921 ggtagaactg ctcggcacca atggtcatca tatggaggat aacctgggca tccttcgggt
     1981 cgtttttatc ccaggaatta tgcaatgcct ccctggtccg agccagcgcg atcgacgaga
     2041 tcagtttgat ctcgaagccg gctgagccga gctgatatgc caatgcgcga tgatagtttc
     2101 ccgttgcttc gaaggctacg cggacaggac gctgatagct gcgcagcaag ctgatgaggc
     2161 gatcgaactc atcaagattg ttgagcacgc tcacccgacg tcggcgcttt ttgtccggta
     2221 cttcgatcag cacctcgtgg cgttctttgg aaatatcgat agctgccaga acgggagcag
     2281 atgctgtaat gtcacaatcg gtcatagccg gtctccttta cagtgtggct tcgcaaaacc
     2341 actgtgagac ctgatgcccg gctatgacca cctgcttcgc ctgattgggg ctacgcaact
     2401 ggtcaaagcc tcttgcaacg caaacttccg aaggtgttat ggcagcgaat tcatctcgcg
     2461 cgacctagac ctctgggcct accataaggg cgtcgagctc gacttctcgc ggcctggcaa
     2521 gccgacggac aacagctaca tcgagagttt caatggcaag ttccgggccg agtgcctgaa
     2581 cgcccattgg ttcatgagcc ttgacgacgc gcgctcgaag atggaggatt ggcgtagaga
     2641 ctataacgag ttccggccac atagcgccat cggcaacaag gtgccgattt cgctgatgaa
     2701 cggctcatcg gcacccccgc cgaac
//
LOCUS       DATMTI010000084         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_724552, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000084 DATMTI010000000
VERSION     DATMTI010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724552"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VN039_01485"
     CDS             complement(<1..1192)
                     /locus_tag="VN039_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV44687.1"
                     /translation="MRRGPSVTASSPNTSGLAMTYDAIIIGGGPAGSTTGLLLARAGW
                     SVALVERQRFPRAKVCGEFVSAPSLQLLHELGIDESLIQSAGPEVRSLGLFVGGKRVV
                     TEMPQAQTPGYQLGRAVPRERLDLLLLTEAVKAGAEIWQPWSLAEVSKKNNSFYCTIR
                     SKESGEADRLSSRLVIAAQGSCGHRSPLQQGELSPRASDLFAFKARFRNCDLSPDLMP
                     LVMFPGGYGGMVYTDQNRTSFSFCIQRNILEQCRHRWPNRKAAEAAFAYIRQSSAVVS
                     QVFAQASLDGQWYSIGPLRPGVWTCGCGGIFFVGNAAGEAHPIIADGINMAIQSAWIL
                     CQFLVKTAPSEMGDQTLTIISRLYAKQWMREFGPRIRSGSLFATIAMSSKNMWGGLLC
                     ERLPR"
     gene            complement(1128..>1646)
                     /locus_tag="VN039_01490"
     CDS             complement(1128..>1646)
                     /locus_tag="VN039_01490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV44688.1"
                     /translation="DGRLMLRLARALDWRGVRLTIVDRQNLLTHETCEAFAALGWSAG
                     IEVADIHDWLARPSSERADVMVANLVLHHFTDTELATCFRHIAKRARVFVACEPRRAR
                     RAIVGARLLWLIGCNAVSLHDAWVSVRAGFMGIELSQLWPSGSHWSLQEHAAGPFSHC
                     FFAQYQRSGNDV"
BASE COUNT          377 a          500 c          449 g          320 t
ORIGIN      
        1 gtcgaggaag gcgttcacag agaagtcctc cccacatgtt cttcgaactc atggcaatcg
       61 tggcgaacaa cgatccgctg cgaattctcg gtccgaactc tctcatccat tgtttggcat
      121 acagacgact tatgattgta agagtttggt cccccatttc ggacggtgcc gtcttgacaa
      181 gaaattggca caggatccac gcagactgga tggccatgtt gatgccatcc gcgatgatag
      241 gatgcgcttc accggccgca ttgccgacaa agaagatccc gccacaaccg caggtccaaa
      301 ctcctgggcg gagtggtccg atggagtacc attgcccgtc aagggaggcc tgtgcaaaga
      361 cctgactcac cactgcgctc gactgtcgaa tgtatgcgaa agctgcctcc gctgctttcc
      421 ggtttggcca acgatgacga cattgctcca gaatattcct ttggatgcag aaggagaaac
      481 tggttcggtt ctgatcggta tacaccattc cgccataccc gcctggaaac ataactaagg
      541 gcatcaaatc cggcgaaaga tcgcaatttc ggaagcgggc cttaaaggcg aagaggtcgg
      601 aagcccgagg cgacagctcc ccctgctgaa gtggtgaacg atgaccacaa gatccctggg
      661 ctgcaatcac gagccggctg gatagccgat cggcctcgcc cgactctttc gagcggattg
      721 tgcaataaaa actgttattc ttcttcgaaa cctcagcgag gctccaaggc tgccagatct
      781 ctgcgcccgc cttaacggct tccgtgagga gcagcagatc gagacgttcg cgtggaaccg
      841 ctctccctaa ttgataacca ggagtttgcg cctgaggcat ctcggtgacg accctctttc
      901 cgccgacgaa gagaccaaga cttctgacct ccggtccagc cgattgaatc agcgattcat
      961 caatgcctaa ctcgtgcagt agctgcaggc tgggcgccga aacgaattca ccgcacactt
     1021 tcgccctcgg gaagcgctgc ctctcaacga gagcaaccga ccatcccgca cgggcgagca
     1081 ataggccggt cgtggaaccg gcaggacctc cgccgatgat gatggcatca tacgtcattg
     1141 ccagaccgct ggtattgggc gaagaagcag tgactgaagg gccccgccgc atgttcctgg
     1201 aggctccagt ggcttccaga tggccaaagt tgactcaatt ctattcccat gaaccctgcg
     1261 cgaacgctga cccatgcatc gtgcagcgag accgcattac acccgatcaa ccagagcagg
     1321 cgcgctccca caatagcccg acgggctcgt cgcggctcgc acgccacaaa gactctggcc
     1381 cgttttgcaa tgtgtcggaa gcaggttgcg agttcggtat ccgtaaagtg gtggagcacc
     1441 aaattggcga ccatgacatc ggcccgctcg ctcgaaggtc gcgcgagcca gtcatggata
     1501 tccgcgacct ctatccctgc gctccaaccc agggccgcga acgcctcgca tgtctcgtga
     1561 gtgagcagat tctgacgatc gacgatcgta agccgcaccc cccgccagtc tagggcgcga
     1621 gcgaggcgga gcataagccg accatc
//
LOCUS       DATMTI010000085         6228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_512594, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000085 DATMTI010000000
VERSION     DATMTI010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512594"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VN039_01495"
     CDS             complement(<1..874)
                     /locus_tag="VN039_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV44689.1"
                     /translation="MADRNKRLDSNVPGNFYVDATCINCDTCRQLAPLSFEEVGDYSA
                     VNRQPEDEKQIHQAYQALLACSVGSIGTEHSDKARLHTAMASFPLRIENGVSYCGFNS
                     EKSFGANSFFIEHPDGNWLIDSPRYIKHLVEAFERRGGIAHIFLTHKDDVADADKYAA
                     HFGAERIIHQADKEAAPAAERVIGGEETVRVGSEFEIIPVPGHTAGCIALLYRERFLF
                     TGDHLWWDPHIKSLEAPTRLIWRRWTLIESIRKLLDYPFEWVLAGHGDRVHLSSAEMR
                     AQLSALVKRRETKGG"
     gene            complement(874..1461)
                     /locus_tag="VN039_01500"
     CDS             complement(874..1461)
                     /locus_tag="VN039_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HWV44690.1"
                     /translation="MRRLIEEVGPFTLKPKRSRSPFESLVRAVAYQQLHDKAAESILK
                     RFIALFPGRRFPRPDELLAVHMRSVRKAGFSRPKILALRDLAAKTIDGTVPTNRMIRR
                     LADEAIIERLIEVRGIGQWTVEMLLIFQLGRPDVLPVDDFGLRNGFRIAYRRSTMPMP
                     KEMLRYGERWRPYRTAAAWYLWRAADREKRAMEVF"
     gene            complement(1515..2465)
                     /locus_tag="VN039_01505"
     CDS             complement(1515..2465)
                     /locus_tag="VN039_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HWV44691.1"
                     /translation="MAEKKIIAVVGATGMQGGGLVRAIVSDPDKTFIARALTRDVHSD
                     KAKALIKLGAEVVSADLDDAAGLKRAFTGAYGVFCLTNFWEHCSPEKEYAQAKNQAEA
                     AKETDVRHVIWSTLEDTRKWVPLSDNRMPTLMGKYKVPHFDVKGEVEREFTKLGLPVT
                     FLLTSFYWDNLIFFGMGPKKGPDGTLLFTMPMGGKKLPGIAVEDIGKCALGIFKSGKE
                     YTGKTVGIAGEHLTGEEMAAAMTKALGQTVRYNLVTPEQYRAFGFKGADDLGNMFQFK
                     SDFNEVYCAARKPAVAKSLNPALQTFEVWLKQNKSRIPLT"
     gene            complement(2514..3245)
                     /locus_tag="VN039_01510"
     CDS             complement(2514..3245)
                     /locus_tag="VN039_01510"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWV44692.1"
                     /translation="MKSLSGRVAIVTGASNGIGRAIAERLADNGAIVVVNYSKSAEKA
                     QQVVAGIQAKGGKALAVQADMSQVTDARRLVVDTVKQFNRLDILVNNSGRFMPKLLEE
                     TTEEDFNSVIALNAKGPYFAMQEAARVLKDGGRIVNISTGGTHLHFPGATAYLGSKAA
                     LEQYTKGLAQELAPKGITVNTVSPGFTETGMMTEEYRQIGIQLSPMKRLGVPKDIADV
                     VAFIVSEEARWLTGQTIQVGGGIVM"
     gene            complement(3261..3863)
                     /locus_tag="VN039_01515"
     CDS             complement(3261..3863)
                     /locus_tag="VN039_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWV44693.1"
                     /translation="MEKPAHTQFPIHELVARRWSPRSFDERPIGADLLRTLFEAARWA
                     PSSNNEQPWRFVVASKENETDWNRLFDCLTEGNRRWAFRAPVLMLSIASIHFEDDGTP
                     NRHAFHDTGLAAENLVLQATASGLVAHQMAGFDVEKARVDLKIPWGLEPVAMIAVGYP
                     GDPTVLPDRLRERELQPRNRWPIQEWTFLGQWGNPIASQD"
     gene            complement(3826..5298)
                     /locus_tag="VN039_01520"
     CDS             complement(3826..5298)
                     /locus_tag="VN039_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWV44694.1"
                     /translation="MRSFLTCRCMALLVLSALLTSCAMGPDYKRPQTDVDDRFRMAEG
                     RSDLPSLANLPWWELLRDEQLQQLIRSALEHNWDLQRAVATIDEFRARALVARTDFLP
                     QIATTVNAPAIRHTAFLVPGFPNLFDYYFLGNLSWELDLWGRIRRSTEGARADLLSKE
                     ENRRAVTIQLVSAVAEAYFNLLQFDLQLDIAERTLRSWEESVRIAQARLHQGMTSKLD
                     ADQFEAERANAAARTEELQRQMVQAENQLSILLGRRPFAVARGRSLDEQILAPDVPPG
                     LPSELLQRRPDVLVAERQLAAATARIGAAKAARFPKITLTGVAGMANPALQSIFTSPG
                     HFGVFGAALAAPIFNAQILGFEQETVEAQSQQAMAQYQQTVLTAFREVEDSLIAVRTA
                     RMQSESQQQQVTALQSALKLAELRYKGGLANYLDVLVARRNLFEAELALTSTRRLLLA
                     SIVQLYRALGGGWSPTGQPDEDPSKDSDHGEARAHPISNP"
     gene            complement(5298..>6228)
                     /locus_tag="VN039_01525"
     CDS             complement(5298..>6228)
                     /locus_tag="VN039_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV44695.1"
                     /translation="RSDPADIGKVYVRSLSPSATAFGGRAGAPGLGGTGPGGVMIPLD
                     TVVDTEFTSGPDPVTHFNGFNTAWVLGAAAPGYSSGQALEALERTAREVLIPKGYTLE
                     WSGISYQEAKVGGQSGLAFGFGLLMVFLVLAAQFESWTVPLAVILAVPFGVFGAMSAV
                     WLRGMTNDIYFQIGLVTLIGLAAKNAILIVEFANHRRTDGMPIQQAAQEAARLRFRAI
                     IMTSMAFVGGVLPLAIATGAGAASRQSIGTGVLGGMLTATFLAIFFVPLFFVTVRKIG
                     ERWAPMKKRVELPEVPPERARREYAGTSVDGDH"
BASE COUNT         1314 a         1945 c         1657 g         1312 t
ORIGIN      
        1 cgccgccctt tgtttcccga cgctttacca atgccgagag ttgcgcccgc atctcggccg
       61 aggagagatg cactcgatcg ccgtgtcctg ccaggaccca ttcaaacggg taatcgagga
      121 gtttccggat ggactcgatc aaggtccatc ttctccaaat gagacgggta ggagcttcca
      181 acgacttgat gtgtggatcc caccagaggt gatcgccggt gaagagaaat cgctcgcggt
      241 agagcaaggc tatgcatccg gcagtgtggc ccggcacggg aatgatttcg aactctgatc
      301 cgacccgaac cgtttcttcc cctccaatca cccgttctgc agccggagcg gcctctttgt
      361 ccgcctgatg gatgatgcgc tccgcaccga aatgcgcggc atatttatcc gcatccgcca
      421 catcatcttt gtgtgtcaaa aagatatggg cgatcccgcc ccgccgctca aaggcttcaa
      481 cgagatgctt gatgtaccgg ggagagtcga tcaaccagtt gccgtccgga tgttcaatga
      541 aaaaactatt cgctccgaac gatttctccg aattgaaacc gcaataactc accccgtttt
      601 cgatccggag gggaaagctc gccatggctg tgtgcaatcg tgccttatcg ctgtgttcag
      661 tgccgatcga gccgacggaa caggcaagca atgcctggta ggcctgatga atctgctttt
      721 catcctccgg ttggcggttg accgccgaat agtcgccgac ttcttcaaag ctcagcggcg
      781 ccaattggcg acaggtgtcg caattgatgc aggttgcatc cacgtagaag ttaccgggaa
      841 cgttggaatc gaggcgtttg tttcggtcgg ccatcagaac acttccatag cccgtttttc
      901 tcgatcggcg gcacgccaga ggtaccaggc ggcggccgta cgatagggtc tccatcgctc
      961 gccataccgt aacatctctt tcggcatcgg catcgtcgac cggcgatagg caatgcggaa
     1021 tccgttgcgc aggccgaaat catcgaccgg caacacatcc ggacgcccca actgaaaaat
     1081 cagcaacatc tcaaccgtcc attggccgat gcctctgact tcgatcagcc gctcgataat
     1141 cgcttcatca gccaatcgcc tgatcatacg gtttgtcggc acagtcccat cgatggtctt
     1201 tgcagccaga tcacgaagcg ccaaaatctt cggtcgcgaa aaaccggctt tcctgaccga
     1261 tctcatatgc acggcaagca gctcatccgg acgaggaaac ctccggccgg gaaagagcgc
     1321 aataaaccgc ttgagaatgc tctcggcggc tttgtcgtgg agctgctggt aggcaaccgc
     1381 gcggaccaac gattcgaacg gcgagcggct cctttttggt ttcagcgtga aggggccaac
     1441 ctcctcgatc aaccgacgca tgacggggtc agcttttgaa agatagtcgg tcggttcaga
     1501 atcgaggtga cccattacgt cagcggaatg cggctcttgt tctgtttcag ccagacttcg
     1561 aatgtctgca atgccggatt caagctcttt gcgacggcag gcttgcgtgc cgcgcagtag
     1621 acctcattga aatcgctttt aaactggaac atgttcccga gatcgtcggc gcccttgaaa
     1681 ccgaacgcgc ggtactgttc cggagtgacg agattatagc gcacagtttg tccaagcgcc
     1741 ttggtcatgg ccgcagccat ctcctcaccg gtgagatgtt cacctgcgat cccgaccgtc
     1801 ttcccggtgt attccttgcc gctcttgaaa attcccaacg cgcactttcc aatgtcctcg
     1861 actgcgattc cgggaagctt tttacctccc atcggcatgg tgaacagaag cgtgccgtcc
     1921 ggccccttct ttggtcccat gccgaagaag atcaaattgt cccaataaaa ggacgtcagc
     1981 agaaaggtca ccggaaggcc caacttggtg aactcccgct ctacttctcc cttcacatcg
     2041 aagtgtggaa ctttgtactt gcccatcaac gtcggcatac gattgtccga cagcggcacc
     2101 catttgcgtg tgtcttccaa cgtggaccag atcacgtgtc gtacatcggt ctccttcgcc
     2161 gcctcagcct ggttcttcgc ctgtgcatat tctttttcgg gggaacaatg ctcccagaaa
     2221 ttagtcaaac agaagacacc gtacgcgccc gtgaaggctc gcttcaagcc ggcggcgtca
     2281 tcaagatcag cggacacgac ctcggcacca agcttgatga gagccttggc tttgtctgaa
     2341 tgtacatccc tcgtcaatgc acgggctata aaagtcttgt ccggatcgct cacgattgcc
     2401 cgcaccagcc ctcctccttg catgccggtc gcccccacga cggcgataat tttcttttca
     2461 gccataatat gcaccctccc ctaggaacag gccgtaaccg gcttccataa gaatcacata
     2521 acgatcccgc cgccgacctg aatcgtctgt cccgtgagcc atcgagcctc ctcgctcacg
     2581 atgaaggcca cgacatcagc tatgtctttc ggcacaccga gccgtttcat cggcgacagt
     2641 tgaatgccga tctgccgata ttcctccgtc atcatcccgg tttcggtaaa acccggtgag
     2701 acggtattca ccgtgatccc cttcggagcc agctcttgag ccaatccctt ggtgtattgc
     2761 tccagcgctg ccttgctccc cagataggcc gtggccccgg gaaagtggag atgcgtcccg
     2821 ccggttgaga tgttcacgat ccgtcctccg tctttcagca ctcttgccgc ttcctgcatg
     2881 gcaaagtacg gtcctttggc attcaaggcg atcacgctgt taaagtcctc ttcagtcgtc
     2941 tcttcaagca gcttcggcat gaatctcccc gagttgttca cgagaatatc cagccggttg
     3001 aattgtttga ccgtatcgac caccagtcgg cgcgcgtccg tcacctgact catgtcggct
     3061 tggaccgcta acgccttgcc gcctttggct tggattcccg caacgacctg ttgagccttt
     3121 tccgcgctct tggaatagtt caccacgacg atggcgccat tgtccgccag gcgttctgca
     3181 atcgctcgac cgattccgtt ggatgccccg gtcacaatcg ccactctccc gctcaatgat
     3241 ttcatccgct cgactcctct ttaatcctgt gatgcgatgg gattccccca ttgtcctaaa
     3301 aatgtccatt cttgaatcgg ccagcgattt cttggctgta attcccgctc ccgcaggcga
     3361 tccggtagaa cggttggatc acctggatag ccgacggcga tcatcgcaac cggttccaag
     3421 ccccaaggaa tcttgagatc gacccgcgcc ttctccacat cgaagcccgc catctgatga
     3481 gcaaccaacc cgctggccgt cgcctgcaaa acgagattct ccgccgccaa ccccgtatcg
     3541 tgaaaggcat gccggttcgg cgttccgtca tcctcgaaat gaatgctcgc aatcgagagc
     3601 atcagcaccg gcgcgcggaa ggcccatctc cggttcccct cggtcaagca atcgaacaag
     3661 cggttccaat ccgtctcatt ttccttgctg gcgacgacaa atcgccacgg ctgctcgttg
     3721 ttcgacgaag gcgcccagcg agccgcttcg aaaagagtcc gcaataagtc ggcacctata
     3781 ggccgctcat caaaggaacg cggactccaa cgacgggcga ccaactcatg gattggaaat
     3841 tgggtgtgcg cgggcttctc catgatcact atctttactt ggatcctcgt ccggttgtcc
     3901 agttggagac catccgccgc cgagcgccct gtagagttgg acgatcgacg ccagaagcaa
     3961 ccggcgtgtg ctggtcaacg ccagttccgc ctcgaacaga tttctccgcg caaccaggac
     4021 gtcaagatag ttggcgaggc cgcctttgta gcgaagttcg gcaagtttca gcgccgactg
     4081 taacgccgtg acctgttgct gctgtgattc gctctgcatg cgggccgtcc gaacggcgat
     4141 aagcgaatct tcgacttcac gaaaggccgt cagcaccgtc tgttgatact gggccattgc
     4201 ttgttggctc tgagcttcca ccgtttcctg ctcgaaaccc agtatttgag cattaaagat
     4261 aggagcggcc aaagcggcac cgaacacacc gaagtggccc ggacttgtga agatcgattg
     4321 caatgcagga ttagccatgc cggccacccc ggtcaacgtg attttgggga atcgtgcggc
     4381 cttggcggcc ccgatacggg cggtggcggc cgccaactgt ctctcggcca ccaacacatc
     4441 gggacggcgc tgcaacagct ccgacggcaa gcctggagga acatccggag ccaggatctg
     4501 ctcatccaat gaacggccac gggcgacggc aaacggtcgc cggcccagca ggatgctcaa
     4561 ctggttttcg gcttgaacca tctgccgttg gagttcctcc gtgcgggcag ccgcattggc
     4621 tcgttccgct tcaaactgat ccgcatccag tttcgaggtc atcccttgat gcaaccgagc
     4681 ctgcgcgatc cgcaccgact cttcccatga tcgcaaggtt cgctccgcga tgtcgagctg
     4741 gagatcaaac tggagaagat tgaaataggc ttcagcgacg gcgctgacca gctgaatggt
     4801 cacggcacgt cggttctcct ccttggacaa caagtcggcg cgagccccct cggtggatcg
     4861 tctgatgcgg ccccacagat ccagctccca cgacaagttg cctaggaagt aatagtcgaa
     4921 caaattggga aagcctggaa ccaagaacgc ggtgtggcgg atggccggcg cgttcactgt
     4981 agtcgcgatt tgcggcaaaa agtcggttcg ggcgaccaga gccctcgccc gaaattcatc
     5041 gatggtcgcg acggcacgct ggagatccca attgtgctcc agagccgatc gaatgagctg
     5101 ctgcagttgc tcatcacgca gaagctccca ccatggcaga ttcgccaacg agggcaggtc
     5161 cgatcgaccc tccgccatgc gaaaccgatc atccacatcg gtctgtggcc gcttgtaatc
     5221 cgggcccatg gcgcaggaag tcaagagagc gctgagtacc agcagcgcca tgcaacggca
     5281 ggtcaagaaa gaccgcatca atggtctccg tccactgaag tcccggcata ctctcggcga
     5341 gcccgctcag gcggcacctc aggcaactcg actcgtttct tcatgggagc ccatcgctcg
     5401 ccgatcttcc gcaccgtgac gaagaacaac gggacaaaaa agatggccag gaatgtggcg
     5461 gtcagcattc cacccaagac accggttccg atggattgac ggctggccgc tcccgctccg
     5521 gtggcgatcg ccaagggcaa cacaccgccg acgaaggcca tcgaggtcat gatgatcgcc
     5581 cgaaaccgca accgggcggc ttcctgtgca gcctgctgga tcggcatgcc gtcggttcgg
     5641 cgatgattgg cgaattccac gatcagaatg gcatttttgg ccgcgagtcc gatcaatgtg
     5701 accaagccga tttggaagta aatgtcgttc gtcattcctc gcagccacac agcggacata
     5761 gcgccgaaga ctccgaacgg caccgcgagg atgaccgcga gcggcacggt ccaactttcg
     5821 aactgcgccg cgagaaccag aaagaccatc agcaagccga acccaaatgc cagcccggac
     5881 tgccccccga ccttcgcttc ttggtaggaa atgccgctcc attccagggt gtagcctttc
     5941 ggaatcaaca cctctcgcgc cgttcgttca agcgcctcca aggcctgacc tgagctgtag
     6001 ccgggagccg cagcccccag cacccacgcg gtgttgaaac cattgaaatg cgtcaccgga
     6061 tcggggccgc tcgtgaactc ggtatcgacc actgtatcga gcggtatcat cacgcccccc
     6121 gggccggtac ctcccagacc cggtgcgccg gctcttccgc cgaacgcggt ggcggaagga
     6181 ctcagcgacc gcacgtacac cttcccgatg tcggcagggt cagaccgg
//
LOCUS       DATMTI010000086         1799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_809547, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000086 DATMTI010000000
VERSION     DATMTI010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809547"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VN039_01530"
     CDS             <1..1294
                     /locus_tag="VN039_01530"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWV44696.1"
                     /translation="GGLQMNCTQNYKIDQVTEQTLVVGIDIAKRTHYACFVDDRGRVL
                     RKSFPIFQSKEGFQQLYKAIQEAMQAFGKSEVIVAVEPTGHYWLNLAYFLEEHGIPLV
                     MVNPAHVCRSKELDDNLPTKHDAKDALVIARLAKDGRFLVPRLLHEIEADLRVGSTLK
                     EKLRKEQTAVKNAIVRWTDRYFPEFWTVFRDLGKTALSVLEWTPLPADMAGRTAEELI
                     EVYRQSKGMKCPQKAKIQALINTAKDSIGVTEGTAMARFEIAALVRRYRQLEAEIAAL
                     DAELKALVQTTMEYQWLKTVDGLGDATIIDLLAEIGSFAHYRDPRQLVKLAGLTLKEN
                     SSGQRKGQKHISKRGRKRLRSVLFRAMIPLIRHNEAFRELHEYYTTRSVNPLTGKQSI
                     VALCRKLLNVLFAICTKKQAFDAERMKQDVLSQVQRAA"
BASE COUNT          450 a          458 c          558 g          333 t
ORIGIN      
        1 aggaggactt cagatgaatt gtacacaaaa ctataaaatt gatcaagtta cggaacaaac
       61 gcttgtcgtg ggcatcgata tcgcgaaacg aacccactac gcctgcttcg tggatgaccg
      121 ggggcgcgtg cttcgcaagt cgttcccgat cttccagtcg aaagaggggt ttcagcagct
      181 gtataaagcg attcaggagg cgatgcaagc gtttgggaag tcagaggtga tcgtcgcggt
      241 ggagccgacc gggcactact ggttgaacct ggcctacttc ctcgaggagc acgggatccc
      301 gttggtcatg gtcaacccgg cgcatgtgtg ccggtcgaaa gaacttgatg acaacctgcc
      361 gacgaaacac gacgccaaag acgccctggt cattgccaga ctggcaaaag acggacgatt
      421 cctcgttccc cggctgctgc acgagatcga agccgatttg cgcgtgggaa gcacgctcaa
      481 agagaagctc cgcaaggaac agacggcggt gaaaaacgcg atcgtccgct ggacggatcg
      541 gtattttcca gagttttgga ccgtgtttcg tgacttgggg aaaacggcgc tttcggtgtt
      601 ggagtggacg ccgcttccgg ctgatatggc cggtcggacc gccgaggagc tcatcgaggt
      661 gtaccggcaa agcaaaggga tgaaatgccc gcagaaggcc aaaattcagg cgttgatcaa
      721 caccgcgaag gactcgattg gggtgacgga agggacagcg atggcccggt ttgagatcgc
      781 cgcgctcgtc cgccgatacc gccaattgga ggcggagatc gctgcactgg acgccgagtt
      841 gaaggcattg gttcaaacga cgatggagta ccaatggttg aaaacggtcg acgggttggg
      901 agacgccacg atcatcgatc tgctggcgga gatcggcagc ttcgcccatt atcgggaccc
      961 gcgccaattg gtgaagttgg cgggcctgac gctcaaggag aactcctccg gccagcgcaa
     1021 agggcaaaag cacatctcca aacggggacg gaaacggttg cgctcggtgc tgtttcgggc
     1081 gatgattccg ctgattcggc ataacgaggc gtttcgcgag ctgcatgagt attatacgac
     1141 ccgatccgtc aatccgctga ccggaaagca gtccatcgtc gccttgtgcc ggaagctgtt
     1201 gaatgtgctg tttgcgattt gtacgaagaa acaagcgttt gacgcggagc gaatgaaaca
     1261 ggacgtcttg tcccaggtgc aacgggcggc ctaaggcctc cccctgcgaa cggaagaatc
     1321 gttggacaac gagatgacac cggagaagct ggcgtgattt tatccattcg accttgagtc
     1381 cccaaaggag cttggccggc ctccgcctga tgacgagacc gaacgaggga atgttggcgc
     1441 gaagacgccc ggagacatgg gagggttcgt cctcatcagc gacgcggaga tccaaagggt
     1501 gcatcgaata cgcttccccc acggcaggaa aatataacca gccgtggccg cgaagcgcac
     1561 cctaggtctg taagatatcc acaaaactga aaaaggatgt aagggatttt gtcgaaaaat
     1621 attttttgga cacccctgag gggccgaaaa gccttgataa atcaacattt ctagagggag
     1681 gtaatgaaat ggccgaaaaa ccagcccaat caaagcaggc cgaggcggct gccggcgaaa
     1741 cgctggagca ggtgaaagag cagctcgctg agctcggcaa gaagcgcggc atcctcaca
//
LOCUS       DATMTI010000087         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_639854, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000087 DATMTI010000000
VERSION     DATMTI010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639854"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..2332)
                     /locus_tag="VN039_01535"
     CDS             complement(212..2332)
                     /locus_tag="VN039_01535"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWV44697.1"
                     /translation="MSTINPNGKEIVSVSTEFCGQTLTLEVNRVGFRTTASVLVKYGD
                     TVVLGTTQIGSRPIHLDYFPLSVDYEEKMYAAGKISGSRFIKREGRPSDEAILIGRII
                     DRPIRPLFPKGFRAEVQVVASVLSMDPNFRPDMVGMIAASSALSISGAPFDGPVAGLR
                     VGRVNGEFKAFLTPEEREQSDLDLVVAGIESGVVMVEAGANEIDEEIVIEAIAWAHEA
                     MQPAIVLQKELAEKIAKEPIEYELVLPNEEIQAAVDEWAHEKLGEKLHHPYPERNEMI
                     NELRWKFHEEMAEKVGEDYEQQRDEYDEAFTMALHKDVRRGIVESQTRPDGRKLDEIR
                     PLSSEVGVLPRAHGSSIFTRGVTQGMNIVTLAPLSYAQMVDTMEVSDGERRYMHHYNA
                     PGYTVGEVRRLGSPGRREIGHGYLAERALIPVLPSEEEFPYAIRSVTEIMSQNGSTSM
                     AATCSSCLALMDAGVPIKRPVSGIAMGLMMDGDTPYVLSDIADAEDFAGDMDFKVTGT
                     EKGITAVQMDMKLHGLPVSVLAEAIRASTPGRQHILQHMLTTLAAPKPTLSPYAPRIE
                     KIKINPEKIGAVIGKGGEVINKITAETGAEIDIKDDGLITVAASDTKNIERAIDWIKS
                     LTEEPEVGRIYTGTVVSIKDFGAFVNILPGLDGMVHISQLSDQRVEKVTDVLHEGQEV
                     KVKLTGIDERGRLSLSLKDAAETV"
BASE COUNT          487 a          675 c          666 g          580 t
ORIGIN      
        1 aatggctggg gttgtgacgc tggttgttgt ggctggaaag aagctggtgg cgccatagga
       61 acctccggtg gcgtaggtgt tggttggacc ggctgctgtg gcggaaccgg cggttggttc
      121 atattctgtg ggtccataaa aatgaatgac tcctttagtt ttctactata ggagtcatta
      181 tacacgaaaa gcttacgctt acgaggctgg cttataccgt ttcggcggca tccttcaaag
      241 acaggctgag acgtccgcgc tcgtcgatac cggtcagctt gaccttcact tcttggcctt
      301 catgcagcac gtcagtcact ttttcgaccc gctggtctga aagctgtgaa atatgcacca
      361 tgccgtccag tccaggcaag atattgacga aggcaccgaa atccttgatg gaaacgacgg
      421 tgccggtata aatccggccg acttcgggtt cttcggtcag gctcttgatc cagtcaatcg
      481 cccgctcgat gttcttggtg tcgctggcgg cgaccgtaat cagaccgtcg tccttgatgt
      541 cgatctcagc gccggtctcg gcagtaatct tgttgatcac ttcaccgccc ttaccgatga
      601 ctgcaccgat cttctctggg ttaatcttga tcttttcaat ccgtggtgcg tacgggctca
      661 gcgtcggctt cggcgcggcc aacgttgtca gcatgtgctg aaggatgtgc tggcggccag
      721 gtgtgctggc gcggatcgct tcagccaaga ctgaaaccgg taggccatgt agcttcatgt
      781 ccatctggac agccgtgata cctttttcgg taccggtcac cttgaagtcc atgtcgccgg
      841 caaagtcttc ggcatcagcg atgtcggaaa ggacgtacgg cgtgtcgccg tccatcatta
      901 gccccatggc gataccactg accggtcgct tgatcggcac accggcgtcc atcagggcca
      961 aacaactgct acaagtggca gccatcgagg tcgaaccgtt ctgactcatg atctcagtga
     1021 cgctgcggat ggcgtacggg aattcttcct cgcttggcag aaccggaata agggcacgct
     1081 cagccaggta accgtggccg atttcgcggc gacccgggct gccaaggcgg cggacttcac
     1141 cgacggtgta gcctggcgca ttgtagtgat gcatgtagcg acgctcgccg tcggagacct
     1201 ccatcgtatc gaccatctgc gcatagctca gcggtgccaa ggtaacgata ttcatgcctt
     1261 gggtgacgcc gcgagtaaag atacttgagc catgagcgcg cggcaagaca ccgacttcgc
     1321 ttgaaagcgg gcggatctca tcgagcttac ggccatccgg tcgggtttga gactcgacga
     1381 taccgcgtcg gacatcttta tgcaaagcca tcgtgaaggc ttcgtcgtat tcgtcacgct
     1441 gctgttcgta gtcttcgccg accttctcag ccatctcttc atggaacttc cagcgtaatt
     1501 cgttaatcat ctcatttcgc tcagggtatg gatggtgaag cttctcgccc agtttttcgt
     1561 gcgcccactc gtcaaccgca gcttggatct cttcgttcgg caggaccagt tcgtattcga
     1621 taggctcttt ggctatcttt tcggcaagct ccttctgcag gacgattgcc ggctgcatgg
     1681 cttcgtgtgc ccaggcgatt gcttcgatca cgatctcttc atcgatctcg ttagcaccgg
     1741 cctcaaccat cacgacgccg ctttcgatac cggccacgac cagatcaagg tcactctgtt
     1801 cacgttcttc aggcgtcagg aacgctttga actcaccgtt cacgcgtccg acacgcaggc
     1861 ctgcaacagg accgtcgaat ggcgcgccgc tgattgaaag cgcgctgctg gccgcaatca
     1921 taccgaccat gtcaggacgg aaattcggat ccatcgagag gacgctggcg accacttgaa
     1981 cctcagctcg gaagcctttt ggaaacagtg ggcgaatcgg acggtcgata atgcggccga
     2041 tcaggatggc ttcgtcactc gggcggcctt cacgtttgat gaaacggctg cctgagatct
     2101 tgccggctgc atacattttt tcttcatagt cgacactcag tggaaaatag tcgagatgaa
     2161 tcggacggct gccgatctgc gtcgttccaa gcacgaccgt atcaccgtac tttacgagca
     2221 cactggcggt agtccggaaa ccgacacggt tgacttcgag cgtcagggtc tggccgcaaa
     2281 actcggtaga aacggaaacg atttcttttc cgttcgggtt aatagtactc atcaaagagc
     2341 ctcctttttc cggcggagag taacaggtgc ggggcgcttg cctatcaagc agccaacatc
     2401 tatcacca
//
LOCUS       DATMTI010000088         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_385096, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000088 DATMTI010000000
VERSION     DATMTI010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385096"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VN039_01540"
     CDS             <1..250
                     /locus_tag="VN039_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HWV44698.1"
                     /translation="VTMLQKLADEQVLFWNTANPIVAYLRDRVFRTLDRNARLRYRVL
                     STTAGLRKEPPFGLMDRLMAAGLLPDYSVRDRAVRGIP"
     gene            255..1010
                     /locus_tag="VN039_01545"
     CDS             255..1010
                     /locus_tag="VN039_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44699.1"
                     /translation="MQSVDWTIDELPEETQKLLKSYVKDVAKAYGNELEGILLYGSAV
                     RGEFLTGRSNLNLILVMSSYDLSMLKKYDTIHKRWSKEQVVVPLFLTAGDLQSASQVF
                     PLEYQDILECHRLLWGQDPFVGLKIDVRYLAAEVLQALRGNLFRLRQRLVEGRSTEEA
                     MIILLSLSITALLPVLRGLQRLLLRPVLAHGEALLNDLESHLEINLTGLRDALLFKRG
                     HISPGQKEIPRLMDRYLESLTKLVTAAEGRITR"
     gene            1007..1759
                     /locus_tag="VN039_01550"
     CDS             1007..1759
                     /locus_tag="VN039_01550"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWV44700.1"
                     /translation="MIPRSLRQKSGVPLLCLTLVLWVDSAHASLYERPKERIPLPSPI
                     GYVSDHAQVVEREWKDRIRSVCIDLEKKSGVEMVIVTVPSIKPYPSAKHYADALYEKW
                     QIGSTQQEHGVMVLVAVQERQAAMALGRKMFPVITPTVRNEVSRDYLQPAIERGHFGE
                     GLYRTAVALATPAQEVRLNTPSRPRIKGLGIWITLGTTVAVVLFFWWLSRPDLRHPYR
                     RIQKGEYWGTGQGGFGGNWGGFGGGTSGEGYR"
     gene            1762..>2069
                     /locus_tag="VN039_01555"
     CDS             1762..>2069
                     /locus_tag="VN039_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44701.1"
                     /translation="MENTSMPVVLATLVVFLVGCSTTGKETQSASTPSQEPTDAVIER
                     YIRAHPEVIEQSLQGLLAKREAELKERHKAALATKQNELLHDPASPVSGNPKGEITLV
                     "
BASE COUNT          475 a          547 c          591 g          456 t
ORIGIN      
        1 ggtcacgatg ctgcaaaagt tggccgatga acaggtgctg ttctggaaca cggcgaatcc
       61 gatcgtcgcc tatctccggg accgcgtctt ccgaacgttg gaccgcaacg cacgactccg
      121 gtatcgtgtc ttatcgacga cagccgggct tcgcaaggag cctccattcg gtctgatgga
      181 tcgcctgatg gcagccggat tgttgccgga ttattctgta cgtgacagag cagtcagagg
      241 cattccgtaa ctccatgcaa tcggtcgact ggacgataga tgaactgccc gaagagacgc
      301 aaaaactatt aaagtcttac gtgaaggatg tcgcaaaagc ctatgggaat gaattggaag
      361 gaattctgct gtatggcagt gcagtgcgag gggaattttt gacgggccgc tccaatctca
      421 acttgatatt ggtgatgtca tcctatgatc tctcaatgct gaaaaagtat gacaccattc
      481 ataagcgctg gagcaaggaa caagtggttg tccccttgtt cctgacggcc ggcgatctcc
      541 aatccgcttc gcaggtcttc cctctcgaat atcaagatat cctcgaatgc catcgactgc
      601 tgtgggggca agatcctttc gtcggtctca agatcgatgt ccggtacttg gccgcagaag
      661 tcttacaggc attacgaggg aatctgttcc gccttcgtca gcgtctcgtg gaaggacgaa
      721 gtaccgaaga agccatgatc atccttttgt cgctctccat caccgccctc ctgccggtgc
      781 ttcgaggcct gcaacggctg ctgttgcgac ctgtacttgc gcatggggag gcgctgctga
      841 acgatctcga atctcatctg gaaatcaacc tgactgggct tcgcgatgcg ctcttgttca
      901 aacgtggaca catctctccc ggtcagaaag agatccccag actcatggac cgatatcttg
      961 aaagtctgac aaaactcgtg actgccgccg aagggcggat cacacgatga tacctcgttc
     1021 gctgagacaa aaaagcggtg tgcctttgtt atgcctgacc cttgtgctgt gggtcgattc
     1081 cgcccacgcc tcattgtatg aacggcccaa agaacgtatc ccgcttccca gccctatcgg
     1141 gtatgtgagt gatcatgctc aggtggtgga gcgagagtgg aaggaccgta tcaggtccgt
     1201 ctgcatcgat cttgaaaaaa aaagcggtgt agagatggta attgtgacgg tccccagcat
     1261 taaaccgtat ccgtcggcca agcactatgc ggacgcgctg tatgaaaaat ggcaaatcgg
     1321 ttcgacgcag caggagcatg gggtgatggt cttggtggct gtgcaggaac ggcaggcggc
     1381 gatggccttg gggcgcaaaa tgtttccggt catcacgccg actgtcagga acgaggtcag
     1441 tcgcgactat cttcagcctg ccatcgaacg gggtcatttc ggggaagggt tgtatcgaac
     1501 ggcggttgca ttggcgacac cggcgcaaga agtacggctc aatactccct cacggcctcg
     1561 aatcaaagga ctcggaatct ggattacgct tggcaccacc gtcgccgtcg tgttgttttt
     1621 ttggtggctc agccgtcccg atctgcgtca tccctatcga cgcatccaga aaggtgaata
     1681 ctggggaacc ggccaaggag gcttcggcgg aaactggggc ggcttcggcg gcggcacgag
     1741 cggggaggga tatcgatgag gatggaaaat acatcaatgc cggttgtcct cgctacgctc
     1801 gttgtgttcc tggttggttg ctccaccacg ggtaaggaaa cccaaagcgc gtcaaccccc
     1861 tctcaagaac ccaccgatgc cgtaatcgag cgctacatcc gtgcccatcc ggaagtgatc
     1921 gaacaatcgc tgcagggatt gctggccaaa cgcgaagcgg aattgaagga acgtcacaaa
     1981 gctgcccttg cgaccaagca aaatgaactg ttgcacgatc cggcatcacc ggtcagcggc
     2041 aatccgaagg gcgagatcac gttggtcga
//
LOCUS       DATMTI010000089         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_639928, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000089 DATMTI010000000
VERSION     DATMTI010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639928"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1621
                     /locus_tag="VN039_01560"
     CDS             74..1621
                     /locus_tag="VN039_01560"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWV44702.1"
                     /translation="MSATGRAVIGNKDTQIDSRRDDTIVRERNAILVSLVCAVVLLVI
                     ILLLAARTFDERDRQEQWLIHSHEVSTALESLLSLLKDAETGQRGFLLTAQSHYLEPY
                     QRAVADFPAAEKKLEQLLANNPEQIRRMAELKQYANERLLELERTIELGRRDPQAALD
                     VVRTDRGKQLMDESRRLVAGMMQTEHDLLLDRAEMRRGSVAGTLQWMIVFLLLTILLI
                     GAAGMVIYRSRRALAQEMALRESGENWSAELVRVVNEKTDALHRLEVVERTLREANKE
                     LEAFTYSVSHDLRAPLRSINGFAKILTEDFGPSLPPEAKRHLHVIQKGASRMGELIDD
                     LLEFSRLSRTALDRQVVDLERVVREVWSELRRQDEVRSAELVVGDLPRCLADRRLIKQ
                     VWVNLLSNALKYSRTRSEPRIEIGWREDEQQPGSVVYWIRDNGVGFDQQYVNKLFGVF
                     QRLHRQEEFEGTGVGLALVRRIVQRHGGRVWAEGRLDEGATFYFTFERIHQHAGAGQD
                     FGLVGRR"
     gene            1578..2021
                     /locus_tag="VN039_01565"
     CDS             1578..2021
                     /locus_tag="VN039_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV44703.1"
                     /translation="MPAPDKISVLLVEDNEDDAELTLRVFRQYHFANHIHVVRDGAEA
                     LDCLFSKGVYAQRNVCEQTKLILLDLKLPKVDGLEVLRRCKSDERTKSIPVVMLTSSR
                     EEQDVIESYKLGVNSYIVKPVDFHQFVEAVKQLNVYWLILNQGLE"
     gene            2023..3663
                     /locus_tag="VN039_01570"
     CDS             2023..3663
                     /locus_tag="VN039_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV44704.1"
                     /translation="MISEPIEYAGVGGRMAQPLRVLLLEDLDTDAELMVHELKRAGFN
                     LVWDRVDTEEALLQCLDRPYDLILSDNSMPSFDAMRALACLQQRRLDIPFVIVSGSIG
                     EEQAVALLRHGAADYIMKDRLERLGSAVGQALEKKRLRDDNQAAHAALQARTGELMRS
                     QERLRALASDLTLTEQRERRKLAGDLHDYLAQLLVAGRMKLRQAVARVAKEPGTPLLA
                     ELDQIFDESLRYTRTLIADLAPPSLQEFGLSHAFQWLSEHMRTHGLTVDVQVGSDRIN
                     LPDDQAILVFQSVRELLLNIVKHAETNRASVTVATSPTELSITVTDGGQGFDHAVVGA
                     PESGSSRFGLFSIDERMRAMGGRFEINSTRGRGTRASMILPHWPASAAPELWEVGKAD
                     QRSGLTTQTSPEQPMVRILLVDDHVMVRRGIKSLLDGHENLRVIGEASDGQQAVKLTA
                     ELRPDVVLMDVNLPVMDGVTATSRIHRDYPDTVTVALSVHDDRQIIDAMTKAGAAAFV
                     SKGSVTEELYEAISRALLSKGKLPDSPRRDKLSLSSKA"
BASE COUNT          790 a          988 c         1171 g          780 t
ORIGIN      
        1 ggttgtttag ccgaagatgg attggaacat ctcgcgcttc tcaaaagata gaggtctatg
       61 gcggcctttc catatgtccg cgacgggtcg tgccgtgatt gggaataaag acactcaaat
      121 cgattcccgc cgcgatgaca cgatcgtgcg ggagcgaaat gcgatactcg tcagcctggt
      181 ctgcgccgtc gtcttgctcg tgatcatatt gctgcttgcg gcccgcacct tcgacgagcg
      241 ggacagacaa gaacaatggt tgattcatag tcatgaagtc agcacggcgt tggaatctct
      301 tttgtctctc ttgaaagacg ccgagaccgg gcagcggggg tttctcttaa ccgcccagtc
      361 ccactatttg gaaccgtacc aaagggcggt ggccgatttc ccagccgcgg agaagaaatt
      421 ggagcaattg ctcgccaaca acccggaaca gatccgcaga atggctgagc tgaagcaata
      481 tgccaacgag aggttgctgg agctggaacg tacgatcgag ttgggacgtc gcgatcctca
      541 agccgccctg gacgtggtgc gcaccgatcg cggcaagcaa ctcatggacg agagccgccg
      601 attggtcgcc ggcatgatgc agaccgagca cgatctcttg cttgatcgcg cagagatgcg
      661 tcgagggtcc gttgcgggca cgttacagtg gatgatagtg tttttgctcc tgacgatact
      721 cttgataggc gctgccggca tggtcatcta ccgaagccgg cgggctcttg cacaagagat
      781 ggcgttgcgt gagagcgggg aaaactggtc ggccgagctc gtacgagtcg tgaacgagaa
      841 gaccgatgca ttacacagat tggaggttgt cgaacggaca ttgcgggaag ccaacaaaga
      901 actggaggcg ttcacctatt ccgtctcgca tgacctgcgc gcgccgttac gatcgatcaa
      961 cggattcgcc aaaatcctga ccgaggattt cggcccatcc ttgcctccgg aggccaagcg
     1021 acatctgcat gtcatccaaa agggcgcaag tcgcatgggg gaactgatcg acgacctgtt
     1081 ggaattttca cggttgtccc gcactgcgct cgatcgtcaa gttgtggatc tcgagcgtgt
     1141 cgttcgagaa gtatggagcg aactacggcg gcaggatgaa gtgcgatcag cggaattggt
     1201 ggtgggggat ttaccgagat gcctggcgga tcggcgcttg atcaagcagg tttgggtgaa
     1261 tctcctctcc aatgcgttga agtattcccg cactcgctca gagccccgga tcgagatcgg
     1321 atggcgagag gacgagcagc agccgggcag cgtggtgtat tggattcgag acaacggagt
     1381 gggattcgac caacaatatg tgaacaaact tttcggcgtg tttcagcggc tgcatcggca
     1441 agaggaattc gaggggaccg gcgtggggct tgcgctggta cgccggatcg ttcaacgcca
     1501 cggcggccgg gtgtgggccg aaggacggct ggacgaggga gcgacatttt atttcacgtt
     1561 cgagaggatt catcaacatg ccggcgccgg acaagatttc ggtcttgttg gtcgaagata
     1621 atgaggacga cgcggaactg acgctgcggg ttttccggca gtatcacttc gccaatcata
     1681 ttcatgtggt gcgggacggg gcggaagcgt tggactgcct gttcagcaag ggcgtctatg
     1741 cgcaacggaa cgtgtgcgag caaacgaaat tgattctctt ggacctcaag ctgccgaagg
     1801 tcgacggact ggaagtcctt cgacgctgca agagcgacga gcggacgaag tccattcccg
     1861 tcgtcatgtt gacctcgtcg cgcgaggagc aagacgtgat cgaaagctac aaactgggcg
     1921 tcaacagcta tatcgtgaag ccggttgatt ttcaccaatt cgtcgaggcc gtgaaacagt
     1981 tgaatgtgta ctggctgatc ctgaatcagg ggctagagtg atatgatatc agaacctatt
     2041 gaatatgccg gtgtgggagg tcgtatggct caaccgctgc gtgtcttact gctggaagat
     2101 ctggacacgg atgctgaatt gatggtgcac gagttgaaac gcgccggatt caatcttgtc
     2161 tgggaccgcg tcgatacgga ggaagccttg ctgcaatgtc tcgatcggcc gtatgacttg
     2221 attctctccg acaattcgat gccgtccttt gacgcgatga gggctcttgc gtgtctgcaa
     2281 cagcgccggc tcgatattcc cttcgtcatc gtgtccggct ccatcgggga agagcaagcc
     2341 gtggccttgt tgcgtcatgg cgcggcggac tacatcatga aggatcgtct cgaacgactg
     2401 ggatccgccg tggggcaagc actcgagaag aagaggttgc gcgacgacaa tcaggcggcc
     2461 catgccgctc tgcaggcgag gaccggggaa ctcatgcgtt ctcaagaacg gttgcgcgcg
     2521 ctcgccagcg acttgacatt gacggagcag cgggagcggc gcaagctcgc tggcgacctc
     2581 catgactatt tggctcaatt gctcgtcgcg ggacgcatga agctgcgaca agccgtggcc
     2641 cgtgtcgcca aggagcccgg aacgcctcta ctcgccgaat tagaccagat tttcgacgaa
     2701 tccttgcggt atacacgcac cctcatcgcc gatctggctc ctccttcact gcaagaattc
     2761 gggttgtctc atgcctttca atggttgtcc gaacacatgc gtacccatgg attgaccgtg
     2821 gacgtgcagg tcgggtcgga ccgaatcaat ctcccggacg atcaagcgat tttggttttt
     2881 cagtccgttc gagaattgct gctcaacatc gtgaagcatg cggagacgaa tcgcgcgagt
     2941 gtgacggtgg ccactagtcc cactgaactg tccattaccg tcacagacgg cggacagggg
     3001 ttcgatcatg cggttgtagg tgctcccgaa tctgggtcat ccagattcgg gctgttcagc
     3061 atcgatgagc gaatgcgggc gatggggggg cggtttgaga tcaattccac gcgcggtcgt
     3121 ggaacacggg cctccatgat tctgccccat tggcctgcct ccgccgcacc ggagctgtgg
     3181 gaagtcggca aagcggacca acgctcgggt ctcaccacgc agacctcgcc ggaacagccg
     3241 atggtccgca tactccttgt cgatgaccat gtcatggtcc ggcgcgggat caaaagcctg
     3301 ctggacgggc atgagaactt gcgggtcatc ggagaggcct ccgatggaca acaggccgtc
     3361 aagttgacgg cggagctgcg gcccgatgtc gtgctcatgg acgtgaatct tcccgtgatg
     3421 gacggggtga cggcgacaag ccggatccac cgggactatc ccgataccgt caccgtcgcg
     3481 ttgtccgtcc atgacgatcg ccagatcatc gatgcgatga cgaaggccgg tgcggcggcc
     3541 ttcgtgtcga aggggtccgt gacggaagag ctgtacgagg cgatcagccg cgcattgctt
     3601 tccaaaggca aattgccgga ctctcctcga agagacaagc tgtctctgtc cagcaaggca
     3661 tagcccgccg cattattagg gaccggctcc gtcgtccgcg acggtgccgg ttccggttcg
     3721 gacggggac
//
LOCUS       DATMTI010000090         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_639941, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000090 DATMTI010000000
VERSION     DATMTI010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639941"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..758)
                     /locus_tag="VN039_01575"
     CDS             complement(72..758)
                     /locus_tag="VN039_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV44705.1"
                     /translation="MEDLFKGSIDRFCEIAIRLSEAKRVLDVGSGQGLLLSFLAELGH
                     ECHALDVKDQPSIYPDTYGKGITFRLCNVEADAIPYPDESFDAVVCCQVLEHFSHSHL
                     PAVQEMKRVLLPGGVLEIDVPNVASFRNRLRLLRGKHITYDYAEHYLHAAPILYKGMS
                     YYPLRHNREFTRGELELLLRSAGFQRYEVSFLRSRRYREGIQRARSVGTAMKDMIPSL
                     RKSLIAFAYK"
     gene            complement(868..2028)
                     /locus_tag="VN039_01580"
     CDS             complement(868..2028)
                     /locus_tag="VN039_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWV44706.1"
                     /translation="MSIMMVILPGIIAFVSAWWLTNRLCSPKSFLSILAHPNERTLHA
                     MPTPQTGGLAVIGSVVISLILAASVLAIIQPSKPVLPKGVTSGSVWIVVSMLLIFVVS
                     FIDDCVGLPAALRLGVQAVSACIIIGGVGLTLSFIPIPGGPHILLGIAAIPVSVLVLL
                     WMANLYNFMDGMDGFAGGMTFFGFGFLAYFGWQAHFPVMLIIATFVAMGALGFLTHNF
                     PPARIFMGDAGSITIGFLAGTLMILGVRDGIFELWVPVMIFSPFIVDATVTLIRRILR
                     RKKIWQAHREHYYQRLVLSGWSHRRTVLAEYGVMILCGGLAVLYHHSTDDVRLIILGA
                     WVGMFFLLGSLVGKLERNRKVACSLRDPAASGKNAEVPVVSVQDPPRVAVKT"
BASE COUNT          569 a          633 c          539 g          405 t
ORIGIN      
        1 tccaagcgca gtcgtcgtcc agcctgccgc tcgtctcgcc tccgcctgca gcctgtctct
       61 cgtccaaccc atcacttgta ggcgaaggcg atcaaggatt tccgaagcga cggaatcatg
      121 tctttcatcg cagtacccac cgacctcgcc cgctgaatcc cttcgcgata acgccgggat
      181 ctcaggaagg acacctcata tctttggaac cccgccgagc gcaggagcaa ctccaactca
      241 ccgcgggtaa actcgcgatt gtgtcgaagg ggataatacg acatgccctt gtacaggatt
      301 ggcgccgcat gaagatagtg ctcggcataa tcataggtaa tgtgttttcc acgcaacaga
      361 cgaagccggt tacgaaaact ggcgacgttc ggcacatcga tctccagcac acctccgggc
      421 aacaggactc gcttcatctc ctggaccgct gggagatgcg agtgcgaaaa gtgttcgagc
      481 acctggcaac aaacgaccgc atcgaacgat tcatctggat agggaatcgc atcggcttcc
      541 acattacaaa gccgaaacgt aatgcctttc ccatacgtat cagggtaaat ggaaggctga
      601 tccttcacat cgagcgcatg acactcatgg cccagttcag ccagaaacga gagcaataag
      661 ccttggccgg acccgacatc gagcacccgc ttggcttcac tcagccggat cgcgatttcg
      721 cagaaccgat cgatgctccc cttgaacaaa tcctccattt ccgggtcgcc tcgtcgaaaa
      781 agacggcctt cattccgcaa ccggacggcg gaatgcaaaa ggcccctgct gtttgtcgat
      841 cgacccacgg caagatcgga gttcaggcta tgtcttgacg gcgacgcgtg gagggtcttg
      901 aaccgacaca acaggcactt cagcgttctt gcctgatgca gcaggatctc gaagagaaca
      961 agcaaccttt cgattccgtt ccaacttgcc caccaagctc ccgagcagga aaaacatgcc
     1021 cacccatgca ccaagaataa tgagtctcac atcgtccgtc gaatgatggt acagaacggc
     1081 cagccctccg cacagaatca tgaccccata ctctgcgagc accgtccggc gatggctcca
     1141 tccgctcaag accaaccgct gataataatg ctcccgatgg gcttgccaga tctttttgcg
     1201 acggagaatc cgtcggatca gggtcaccgt cgcgtccaca atgaatggtg agaaaatcat
     1261 gaccgggacc cacaactcga atattccgtc gcgaacccca aggatcatca atgtccctgc
     1321 caagaatcca attgtaatgc tccccgcatc gcccatgaag atacgtgcag gcgggaaatt
     1381 atgggtgaga aatcccagcg ctcccatcgc aacgaacgtg gcgatgatga gcatgaccgg
     1441 gaaatgggcc tgccacccaa aatatgcgag aaaaccgaac ccaaaaaacg tcatgccgcc
     1501 cgcaaaacca tccataccgt ccataaagtt atagaggttc gccatccaga ggaggacaag
     1561 gacactcacc gggattgcgg caatcccaag caggatatgc gggcctccgg gtattgggat
     1621 gaatgacaag gtcaagccga cacccccgat aatgatacag gcggagactg cttggacacc
     1681 caatcggagg gcggcgggaa gaccgacaca gtcatcgatg aaggacacga caaagatgag
     1741 gagcatcgaa accacgatcc agacgctccc tgacgtcaca cctttcggca acacaggttt
     1801 cgagggttgg ataatcgcca acacactggc agccagaatg agactgatca ccacactgcc
     1861 gatgacggcc aaccctccgg tctggggagt cggcatagca tgcaatgttc gttcgttggg
     1921 atgagcaagt atcgagagga acgatttcgg cgaacagaga cggtttgtga gccaccaggc
     1981 cgatacaaag gcaatgattc ccggaaggat gaccatcatg atcgacatat cgaatgctac
     2041 cttattagat gccgctctaa cagccccgcc ctggactcaa gagattttgc cgtttgttcg
     2101 gcaagctcta tgactcattt ttacattgaa atcaagacgt ccgagc
//
LOCUS       DATMTI010000091         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_88235, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000091 DATMTI010000000
VERSION     DATMTI010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88235"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VN039_01585"
     CDS             <1..489
                     /locus_tag="VN039_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWV44707.1"
                     /translation="AQEEKRTAELDSEIRHTKAELAEFEVRNRELSAQVQVAREQMGR
                     LQEETEAIREATVLERKALEDMQRKGPSLSSKPKKVEASKAVSGGRSGGSLSDKGMAA
                     VTEPASAMKVTPGMVHVVKPGETLFSIGRRYGVEVDKLKKLNKLPDDIVEIGQKLLVG
                     AE"
     gene            500..1996
                     /gene="trpE"
                     /locus_tag="VN039_01590"
     CDS             500..1996
                     /gene="trpE"
                     /locus_tag="VN039_01590"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HWV44708.1"
                     /translation="MKAATYSLTLDEFRSYAKQGNLIPLFREILADHDTPVSAFAKID
                     HGPSAYLLESIQGGEKWARYSFLGSGAPLVIYEDRGDLCVKKGSDRRRIPSRGAPLDR
                     LREIMETYRPVTVPDLPRFVGGAVGYLGYDLVRTFEDLPFRRKEHLDIPDFAFLLTET
                     LLIFDNVSQKIKVVANAHVKSSSERDIHSAYRDATGRIEAMIARIRRPLRRVKPRRRR
                     SPIRFMANMSKADFEKIVSHAQEYIKAGDIFQCVLSQRWETNLQTPPFQLYRALRLVN
                     PSPYMYYLRIAGVELVGSSPEILVRCEDGLVSIRPIAGTRRRGATMDEDAELERRLLA
                     DAKERAEHIMLVDLGRNDVGRVAERGSVHVESLMNVERYSHVMHMVSNVTGKLCPSKT
                     VYDVLKACFPAGTVSGAPKIRAMEIIEELEPTRRGPYAGAVGYVGFSGNMDMCINIRT
                     VVVSRRRAFIQAGAGIVADSNPEHEYEETCNKSRAMMKAIELAEQGLE"
     gene            2100..>2609
                     /locus_tag="VN039_01595"
     CDS             2100..>2609
                     /locus_tag="VN039_01595"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWV44709.1"
                     /translation="MLLVIDNYDSFTYNLVQYLGELGEDVQVYRNDKITLDQIEELQP
                     SRLVISPGPCTPREAGISVDAIRRFGGNRPILGVCLGHQSMAVAYGGEVIRAPRLMHG
                     KTSQIKHDGKTIFQSLPNPFEATRYHSLIVNRVNLPDCCEISAETAEGEIMGLRHKTL
                     GVEGVQFHPE"
BASE COUNT          593 a          669 c          782 g          565 t
ORIGIN      
        1 gctcaggaag agaagcgaac ggctgagctc gacagcgaaa tcaggcacac caaggctgaa
       61 ctggctgaat ttgaggtgag aaatcgcgag ctgtccgcac aggtgcaggt agcacgagag
      121 cagatggggc gcctccaaga agagacggaa gcgatccggg aagcgaccgt gttggaacga
      181 aaagccttgg aggatatgca gcggaaaggc ccttctcttt cctctaaacc gaaaaaagtc
      241 gaggcgtcga aagctgtttc cggcggtcgc agcggaggca gtctttccga caaaggtatg
      301 gcagctgtga ccgagccggc ttctgccatg aaggtaacgc cggggatggt gcatgtggtg
      361 aagccggggg agactctctt cagcatcggg agacggtatg gagtcgaggt cgataagttg
      421 aagaagctga ataagctgcc ggatgacatc gtcgagatcg gacagaagct cctggtggga
      481 gccgagtaag cagaacgaca tgaaggccgc gacctattca ctcactctgg atgaatttcg
      541 ctcgtacgca aaacagggca atctcattcc gttgttccgt gaaatcttgg cggaccatga
      601 tacgccggtc tcggcctttg ccaagatcga tcacggtccc tcggcctatc tgctggagag
      661 tatccaaggg ggcgaaaagt gggctagata ttcctttctt gggagtgggg ctccgctcgt
      721 catctacgaa gatcgtggcg atctttgtgt gaagaagggc tcggatcgcc ggcggatccc
      781 tagccgaggc gcgccgctgg accgtctgcg ggagatcatg gagacatatc gccccgtcac
      841 agttccggat ctgcctcgtt tcgtaggtgg agccgtcggt tacctcggct acgatttggt
      901 gcggacgttc gaggatcttc cctttcgaag aaaggagcac ctcgatattc cggactttgc
      961 ctttctattg accgaaacgc tgctcatctt cgacaacgta tcgcagaaaa tcaaggtcgt
     1021 cgccaatgca catgtaaagt cctcgtcgga gcgagatatt cattcggcct atcgtgatgc
     1081 gacaggcagg attgaagcga tgattgccag aatccgtcga ccgcttcggc gggtgaagcc
     1141 gaggcgtaga cgatctccaa ttcgtttcat ggccaatatg agcaaggcgg actttgagaa
     1201 gatcgtttct cacgcccagg aatacatcaa ggccggagat atctttcagt gtgtcctgtc
     1261 gcagcgctgg gaaacgaatc tgcagactcc tccctttcaa ctctatcggg ccttgcgcct
     1321 cgtgaatccg tcgccataca tgtattacct ccggatcgca ggggttgaac ttgtgggctc
     1381 gtctcccgaa attcttgtgc ggtgtgagga cgggcttgtc tcgatacgtc cgattgccgg
     1441 aaccagacgg cgtggcgcga cgatggatga ggacgcggaa ttggagcgcc gtcttcttgc
     1501 cgatgccaag gaacgagccg agcatattat gctggtggat ctcggacgca atgacgtcgg
     1561 ccgtgtggct gaacgaggat ccgtccacgt cgagtcgttg atgaatgtcg agcggtactc
     1621 gcacgtcatg catatggtct cgaatgttac aggcaagctg tgcccgagca agacggtcta
     1681 tgatgtgctg aaggcgtgct ttccagccgg gaccgtgtcc ggtgccccca aaatcagagc
     1741 gatggaaatc atcgaggaac ttgagccgac cagacgcggg ccttacgccg gcgccgtcgg
     1801 ctacgtcggg ttttcgggca acatggatat gtgcatcaat atccgcacgg tcgtcgtttc
     1861 acgccgacgg gcctttattc aggccggcgc gggcatcgtc gccgactcga atccggagca
     1921 tgagtacgaa gaaacgtgca acaagtcccg cgccatgatg aaggcgatcg aactggcgga
     1981 acagggactg gaataggaga gagcctatgg cgaatggcat atggccgata gcaagaggat
     2041 gtcaggtgct ttcctctgtt cgtgctatta gccatcagtt ataagctctt attgcgatca
     2101 tgctgttagt catcgataac tacgactcct ttacgtataa cctcgttcag tatctcggag
     2161 aattaggtga agatgtgcaa gtctatcgaa acgacaagat tacgctcgac cagatcgagg
     2221 aattgcagcc aagccgtctc gtgatttcac cgggaccgtg cacaccacgg gaggccggta
     2281 tttcagtcga tgcgatccgt cggtttggag ggaaccggcc cattctcggc gtctgtttgg
     2341 ggcatcagtc gatggcggtt gcctacggag gagaagtcat ccgcgctccc cgcttgatgc
     2401 atgggaagac gtcgcagatc aagcacgacg gcaagaccat cttccaatca ctacccaacc
     2461 catttgaggc gacacgctat cattctctca tcgtgaatcg ggtcaatctt cctgattgtt
     2521 gtgagatttc cgccgagacc gccgagggtg agatcatggg actccgccat aaaacactcg
     2581 gtgtggaagg ggtccaattc catcccgaa
//
LOCUS       DATMTI010000092         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_809735, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000092 DATMTI010000000
VERSION     DATMTI010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809735"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VN039_01600"
     CDS             <1..783
                     /locus_tag="VN039_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44710.1"
                     /translation="VELLLIAQKDKAPKKAKEYWTLAQTAVDVMLHVPKSPKYIPRKV
                     AAIGQELRQLETQTLLYEGAEASAEAVRLVQTAEESIDAIMERWDIWKKGEALYPSYL
                     LANQAAVKRGVVIRRLLVLTREQIQQESVITNAIQVMDDQRRAGIKIFFAFREDLERA
                     AAFQRFEEDYRQQGAARDLNTAIFDREILIFSRSYGQVQLGIVGPTTPITMINQLEIT
                     WKSELLRDLDPAPLFDMTRYVFDYSGPRAFTVELERFTRAIT"
     gene            780..>2126
                     /locus_tag="VN039_01605"
     CDS             780..>2126
                     /locus_tag="VN039_01605"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HWV44711.1"
                     /translation="MKFPSEPFKIKVVEPIRRTTREERDKLLRNAGYNLFHVPAESVY
                     VDLLTDSGTAAMSDRQWAGLMLGDESYAGSRNYYHLEETVRSIFGYKHVIPTHQGRMA
                     ENLLFSTIVKPGMCIPNNIHFDTTRANVEHQGAEALDVVIKEAYDPHCELPFKGNIDL
                     PRLEETINRVGPDKIPLVMMTITNNSGGGQPVSMDNIRRARGLLDRYHIPLFFDACRF
                     AENCFFIKEREPAYADTSIFDIARELFKYGDGCTMSAKKDGLVNIGGFLSLNDGQWAQ
                     DITNMLILVEGFPTYGGLAGRDLEAMARGLEEVLDEEYLRFRIGQVRYLGELLEGGGV
                     PILKPIGGHAVYLNAKEFLSHIPQSAFPGQALAVALYRDFGIRGVEIGTLMFGKKDPV
                     TGRTIHPELEMVRLAIPRRVYTNMQITYVAESIVELYRRRELIHGLALTYEAPTLRH"
BASE COUNT          505 a          613 c          598 g          410 t
ORIGIN      
        1 gtcgaactcc tcctgatcgc tcaaaaagac aaagccccga aaaaagccaa agagtactgg
       61 accttggcac agaccgccgt cgacgtcatg cttcacgttc cgaaaagccc gaaatacatc
      121 ccacggaagg tggccgccat cggacaggaa ctccgccagc ttgaaacgca gaccttgctc
      181 tatgaaggag ccgaagcaag cgcggaagcc gttcgactcg tccagacagc tgaagaatcc
      241 atcgacgcga tcatggaacg atgggacatc tggaaaaagg gagaggcgtt atacccaagc
      301 tatctcctgg cgaaccaggc ggcggtcaag cggggcgtcg tgatccgccg cctcttggtc
      361 ctgacgcgcg agcaaataca gcaagagtcg gtcatcacga atgccatcca agtcatggac
      421 gaccagcgcc gggcaggcat caagatcttc tttgcattcc gcgaagatct cgaacgagcg
      481 gcggcttttc aacgtttcga ggaggactat agacagcagg gggctgcgcg ggatctcaac
      541 actgcgatct ttgaccggga aattctcatt ttttcccgat cctacggcca agtgcagctc
      601 ggaatagtgg gtcccaccac acccattacc atgatcaatc aattggaaat cacgtggaag
      661 tcggagttgc tgcgggacct tgatccggct ccgctcttcg atatgacccg atacgtcttc
      721 gactattccg gcccacgggc tttcacggtg gaattggagc gattcacgag agcgatcaca
      781 tgaaattccc gtcggagccg ttcaagatca aggtggtgga gcccatacgc cgaacgacgc
      841 gcgaagagcg tgacaagctg ctgcgcaacg ccgggtacaa tctgtttcat gtcccggctg
      901 agagtgtcta cgtggatttg ctcaccgata gcggcacggc agccatgagt gaccggcaat
      961 gggctggact catgctaggg gatgagtcct atgccggcag cagaaactac taccaccttg
     1021 aagaaaccgt ccggtcgatc ttcggctaca agcacgtcat tccgacgcac caggggcgca
     1081 tggccgagaa tcttctcttc tcaacgatcg tcaagccggg catgtgcatt cccaacaaca
     1141 tccacttcga cacgacgagg gccaacgtcg agcaccaggg tgcagaggcg ctcgacgtgg
     1201 tgatcaaaga ggcctatgac ccgcactgcg agttgccctt caagggcaac atcgatctgc
     1261 cccgattgga ggaaacgatc aatcgagtcg ggccggacaa gattccgttg gtgatgatga
     1321 ccatcacgaa caacagcggc ggcggacaac cggtttcgat ggacaatatt cgacgggccc
     1381 gtgggctact ggatcgctac catattcctc tattcttcga tgcctgccgc ttcgcggaga
     1441 actgcttctt catcaaagag cgtgagccgg cgtacgccga tacatcgatc ttcgacatcg
     1501 cccgggaact cttcaagtac ggcgacggct gcacgatgtc cgcgaagaaa gacggcctcg
     1561 tgaacattgg aggatttctc agcctgaacg acgggcagtg ggcgcaggac attaccaaca
     1621 tgctcatcct ggtcgaaggc tttccaacgt acggcggatt ggcgggccgt gacctggaag
     1681 cgatggccag aggacttgag gaggttctcg acgaagaata tttgcggttc cgtatcggcc
     1741 aggttcgcta cctgggcgaa ttgttggaag gaggcggagt accgattctc aaacccatcg
     1801 gcggccatgc ggtctatctc aatgccaaag aattcctctc ccacattccg cagtccgcat
     1861 ttccaggaca agcgctggct gtcgcgctgt accgggactt cggcatccga ggagtcgaaa
     1921 tcggcaccct catgttcggc aaaaaggatc ccgtgacggg ccgaacaatt catcccgagt
     1981 tggagatggt ccggctggcc attccccggc gcgtctatac gaacatgcaa atcacctatg
     2041 tcgcggaatc gatcgtcgag ctctatcgaa gacgcgaact gatccacggc ctcgcgttga
     2101 cctatgaggc accgacgctg cgccac
//
LOCUS       DATMTI010000093         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_300856, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000093 DATMTI010000000
VERSION     DATMTI010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300856"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VN039_01610"
     CDS             <1..825
                     /locus_tag="VN039_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWV44712.1"
                     /translation="DANRLKELGDSIRRHGIMSPLIVEENGERFTLIDGERRYRASKN
                     IRLDEVPVVIRPKNTMTTRLIEQFHIQEQHEGWTGVEKAIAVNDLANQLKTSVMDMAR
                     TLSIPDRTAGEYLAFSKILEKNAYQKGEIPISYAARIVTLREFVKRTWLRHNKEFTED
                     KERELEKAIMARIKDGTLKRKQDIVKIQDAVKTDHTIILKFIKDSKMTVDGLFLASNA
                     KSAEAVRQLRYSLSGIIMHSKQAIALGGMKQMKDEEGAKNQVERAIEALRTVKQYL"
     gene            868..1368
                     /locus_tag="VN039_01615"
     CDS             868..1368
                     /locus_tag="VN039_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44713.1"
                     /translation="MAILRFTSIKERQLFERDKQLIDNELDLYRKRRMLEVEKDILNA
                     RVELQRHLEESRKATHDAEHEYEHEFHENREILRTELVALDAEIKHKKELLGRADSIP
                     ELEYRAFKAEADAQSNEAVIKQLNTDVKRLNDLVEFLAGMLPKVELDKIGVTLNADVK
                     VSGEKK"
     gene            1604..1837
                     /locus_tag="VN039_01620"
     CDS             1604..1837
                     /locus_tag="VN039_01620"
                     /inference="COORDINATES: protein motif:HMM:NF025456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV44714.1"
                     /translation="MEPTQRERVLKRLIEEGEVDNFWAFHSYILRLGAIIHGLRKEGY
                     TIESVYGKERGYARPFWKNCYYILKSKPHVDVF"
     gene            2183..2263
                     /locus_tag="VN039_01625"
                     /pseudo
     tRNA            join(2183..2220,2226..2263)
                     /locus_tag="VN039_01625"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /pseudo
                     /anticodon=(pos:2217..2219,aa:Tyr,seq:ata)
BASE COUNT          653 a          612 c          690 g          404 t
ORIGIN      
        1 gatgccaacc ggctcaagga gctgggggac tcgatacgtc gccacggcat catgtcgccc
       61 ctcatcgtcg aggagaacgg cgaacgcttc accctcatcg acggcgaacg ccgctaccgc
      121 gcatcgaaga acatccgtct cgacgaggtg ccggtggtta tccgtcctaa gaacaccatg
      181 acgacccgtc tcatcgagca gttccacatc caggaacagc acgagggatg gacgggcgtc
      241 gagaaagcaa tcgccgtgaa cgaccttgcg aatcagctca agacgtcggt catggatatg
      301 gcgcgcacgc tctctatccc tgaccgcacg gcaggcgagt acctcgcctt ctcgaagatt
      361 ctcgagaaga acgcgtacca gaaaggagaa atacccatca gctacgccgc gcgcatcgtc
      421 accctccgcg agttcgtcaa gcgtacatgg ctgaggcaca acaaggagtt cacggaagac
      481 aaggagcgcg aactcgagaa ggcaatcatg gcgcgcatca aggacggcac gttgaagcgg
      541 aagcaagaca tcgtgaaaat ccaggatgcg gtcaagaccg accacacaat catactcaag
      601 ttcatcaagg attcgaagat gacggtcgac ggcctctttc tcgcatcgaa cgcgaaatcg
      661 gccgaggcgg tgcgccagct caggtattct ctcagcggta tcatcatgca cagcaagcag
      721 gcaatcgcgc tcgggggcat gaagcagatg aaggacgagg agggtgcgaa gaatcaggtc
      781 gagcgtgcta tcgaggcgct gcgtacggtg aagcagtacc tatagcagaa gctagcctta
      841 gcagttaatc actcataaat aacacatatg gcgatactca gattcaccag tatcaaggaa
      901 cggcaactgt tcgaacgcga caagcaactc atagataatg aattagacct gtatcgcaag
      961 cgtaggatgc tcgaggtaga gaaggatatt ctcaacgcgc gagtggaatt gcaaagacat
     1021 cttgaggaat cgcgtaaagc aacacatgat gccgagcacg agtatgagca tgagttccat
     1081 gaaaataggg agatactccg tacagagctt gtggcccttg atgctgaaat caagcacaag
     1141 aaggaactcc tcggaagggc tgacagcata ccggaactcg agtatcgcgc gtttaaggcc
     1201 gaagcggatg cgcaaagcaa cgaagccgtc atcaagcaac tcaacacgga cgtaaaacgg
     1261 ctcaatgacc tcgtggagtt cctcgctggg atgctgccga aggtcgaact cgacaagatt
     1321 ggcgtcacgc tcaatgcaga cgtcaaggtg agcggtgaga agaaatagaa ggcagtatgg
     1381 catacccgta ctggtacaag tacaagcagc aagtgtggat gacgaacgcg ccccaagcgt
     1441 ttataccccc ccattcatgc aacgtgcagg caacagaaag gcagaggcgg ttctatcagg
     1501 gagtgctcgc ctaccaccgg cggaagaatc cggaactcgt ctcgaaactc gagagcgatt
     1561 gtagcaagcc tgcgtgtgtc cttgacaggc tcaggaagta gccatggaac cgacccagcg
     1621 agaacgcgtc ctcaagaggc tcatcgaaga aggcgaggtc gacaatttct gggcgttcca
     1681 tagctacatc ctgcggctcg gggcaatcat tcacggattg cggaaagagg ggtacactat
     1741 agaaagtgtg tacgggaagg aacgcgggta cgcgcgaccc ttctggaaga actgttatta
     1801 catcctaaaa tccaagccgc atgttgacgt tttctagcag gaacaagtac aaggagttcg
     1861 ccgacccagc gtgcgacgtc tgccagggag aagggcgtgt cgaagacgag cgcataggct
     1921 ctgacggcga accccattac tactggattc cttgcgtgtg caccgaagga aacgtcaaat
     1981 aaggcacgga tagcggtacg agatatgtct aagaaaaaaa gagaacgggc gagcgagcgc
     2041 atacgcgtct acccctactc attgaaggag atacgcttgc gggcgcgtcg ccggataggg
     2101 ggccgtacgc aagcgatggt gattgcggac gcactcatga aagcgaagct atgggaggaa
     2161 tctaacgaaa tggcgtagcg gttgcggggg agcgagctgg tgaacgcgcc tcccttataa
     2221 ggtataagga tggctctggt gggttcaatt cccatccccg caaccctctc accacggcgc
     2281 ggaataccgg gcgcacgagg ccagaaatgg tacaggcgtg gcgagagccg taaacccgac
     2341 gcgctacggg taggacaat
//
LOCUS       DATMTI010000094         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_470561, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000094 DATMTI010000000
VERSION     DATMTI010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470561"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /gene="argC"
                     /locus_tag="VN039_01630"
     CDS             <1..1001
                     /gene="argC"
                     /locus_tag="VN039_01630"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWV44715.1"
                     /translation="ELVRLAAAHPYFTITAVTSEKSAGQSVSSVFPSFKGIVDHQFEA
                     LTPEALTERADAIFLALPHTKSQDPVAACIKAGKPVVDLSADYRLKDVGIYEKWYHAP
                     HRHPHLVQDAVYGLPELHRAAIAKSKLVASPGCYPTAAVLQLAPLFAKRLAQPETIVI
                     DAKSGVSGAGRSPALAYHFPEAHESLEPYKIGQHRHIPEIEQELSGLMDNVGSVIVTF
                     TPHLVPMNRGILSTAYCKLTRPMQLSDLRDLYREFYAAERFVRLYEDLIPNPRYIKGT
                     NYCDIGVYQDSRTGWVVTVAAVDNLVKGAAGQAIQAMNLMLGIPEDTGLTAPGSYP"
     gene            1021..2226
                     /gene="argJ"
                     /locus_tag="VN039_01635"
     CDS             1021..2226
                     /gene="argJ"
                     /locus_tag="VN039_01635"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWV44716.1"
                     /translation="MTPEHIGITAPLGFQAAGIHCGIKKPDLPDLALCVSDVSGPIAG
                     VFTKNRVVAAPVLLDRHHLRFRRGRAIIVNSGNANACTGKQGLVAAKTMAMAVAKQLS
                     IPVHQVFVGSTGVIGRVLPIDRITAAVPALIARLSIPGGDQAAQAILTTDLHPKTVAR
                     QARIGGRVVTIGGMAKGSGMIHPNMATMLAYLTTDAAIAPAALQSALKSAVDQSFNCI
                     TVDGDTSTNDTVLCLSNGLAENRPVQQGTKPYQDFARLLTDAARELALMICRDGEGVT
                     KVVTIRVQGAATTAAAKRVADTIATSNLVKTALFGEDANWGRVMAALGRSGVAIDPAK
                     VTVRFNDIVMVQRGIGMGQQAEQKIAQVFKQKEFTVIVQLGQGHAHAHRWTTDLSYDY
                     VRINASYRS"
     gene            2442..>2827
                     /gene="rpsB"
                     /locus_tag="VN039_01640"
     CDS             2442..>2827
                     /gene="rpsB"
                     /locus_tag="VN039_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWV44717.1"
                     /translation="MGVVAIKELLEAGVHFGHQTNRWNPKMKKFLFGERSGIYIIDLQ
                     QTAVRMEHTYAFVRDLVAAGESVLFVGTKRQAAEILEEEAKRANMFFVNQRWLGGMLT
                     NFQTIRRSIDKMKKMETTLLNPNEHGL"
BASE COUNT          671 a          789 c          771 g          596 t
ORIGIN      
        1 cagaactagt tcggcttgcg gcggcccatc cctattttac gatcactgcc gtgacttctg
       61 aaaagtcagc gggtcagtcg gtctcatccg tatttcccag cttcaaggga atcgtcgacc
      121 atcaatttga agccttgacg ccggaagctc tgacggagcg tgcggatgct atttttctcg
      181 ccctccctca tacaaagtcg caggaccctg ttgcagcctg tatcaaagca ggcaagcccg
      241 tcgtcgacct cagtgcagat tatcggctga aggacgtcgg catctatgag aagtggtacc
      301 atgctccaca tcgtcatcca catttagtcc aagacgcggt atatggtttg ccggaacttc
      361 atcgagccgc aattgccaaa tcgaaactgg ttgcttcgcc cggatgttat cccactgccg
      421 cagttctaca gttggcgccc ctcttcgcca aacggctcgc gcagcctgag acgattgtga
      481 ttgacgccaa gtccggcgtg tcgggagcag gacgaagtcc ggctctggcg taccatttcc
      541 cggaagcgca tgaatcccta gagccctaca aaataggcca acatcgccat attcctgaaa
      601 tcgaacagga actttccggg ctcatggaca acgtcggctc cgtgatcgtg acgttcacac
      661 ctcatcttgt accgatgaat cgaggcatcc tgagcacggc atactgcaaa ctcacgcgac
      721 ccatgcaact ttctgatctt cgggacctgt accgagagtt ctatgcagct gaacgcttcg
      781 tccgactcta cgaagatctc attcccaatc cgcgttacat taagggaacg aactattgcg
      841 acatcggcgt gtaccaggat tcgcggaccg gatgggtcgt caccgtggcg gcggtggaca
      901 atctcgtcaa gggagccgcc ggtcaggcca ttcaagccat gaatctgatg ctcggcatcc
      961 ctgaggacac gggccttacc gcaccgggta gttatcccta accatcctcc tcgaaatcgc
     1021 atgacaccag aacacatcgg catcacggca ccgctggggt ttcaagccgc aggaatccat
     1081 tgtggaatca aaaaaccaga tctgcccgat ttagccctct gtgtatccga cgtcagcgga
     1141 ccgatcgccg gggtgttcac aaagaatcgc gttgtggccg cgccggtact cctggatcga
     1201 caccaccttc ggtttcgtcg tgggcgggct atcatcgtca acagcgggaa cgccaatgcc
     1261 tgcacaggta aacaaggatt ggtggcggcg aaaacaatgg ctatggcagt ggcgaagcaa
     1321 ctttccattc ccgtgcatca agtattcgtg ggttcgaccg gtgtgatcgg tcgtgtgctg
     1381 ccgattgatc gcatcaccgc agctgtcccc gcattgatcg ctcgtctcag catcccagga
     1441 ggcgatcaag cggcgcaagc aattctaacc acagacttgc atcccaaaac cgtcgcgaga
     1501 caagcaagaa tcggcggtcg cgtcgtcacc atcggcggca tggccaaagg ttcgggcatg
     1561 atccatccga atatggccac catgcttgcc tatttgacga cggatgcagc gattgctccg
     1621 gctgccctcc agtcggcatt gaaatcggca gtcgatcaat cctttaattg cattacggtg
     1681 gacggtgata ccagcacgaa cgatacggtt ctgtgcctgt ccaacggcct cgccgagaac
     1741 cgacctgttc aacaaggcac caagccctat caagacttcg cacgactctt gactgatgca
     1801 gcacgagaat tggctctcat gatttgccgc gatggagaag gagtcaccaa ggtcgtcacg
     1861 attcgggtgc aaggagccgc cacgacagca gcggcgaaac gagtcgctga caccattgcg
     1921 acatcaaatc tggtcaagac cgccttattt ggggaggatg ccaattgggg cagggtcatg
     1981 gctgcccttg gacgatcagg cgttgcgatc gacccggcta aggtaacggt acgattcaat
     2041 gacattgtca tggtccagag ggggatagga atggggcagc aggcggagca gaagatcgcg
     2101 caggtcttca agcaaaagga gtttacggtc atcgtccagc ttggacaggg tcacgctcat
     2161 gctcacaggt ggacaacaga cctctcgtat gactatgtcc gcattaacgc gagctatcgc
     2221 tcctagctca aaaagactgt gggcaacttg aaacctcggc aggactatgg tagcgttctc
     2281 ggtctggatt cgacagggag ttcgttttcg gaagaatgga aaaacggccc cattcaataa
     2341 aatcactatc agcgtcgata cgctgcgcag cttgagaata agggaagcga tttcctcgcc
     2401 cgatgcacgg gtgctctgca agcttgaagg gaggtgaagg catgggagtg gtggcgatca
     2461 aggagttgtt ggaagccggc gtccatttcg ggcaccagac caaccgctgg aatcccaaga
     2521 tgaagaagtt tttgttcggt gaacgcagcg gcatctatat catcgatctt cagcaaaccg
     2581 ccgtgcggat ggagcatacc tatgccttcg tccgagatct tgttgcagcc ggagaatccg
     2641 tcttattcgt cggcacgaag cggcaggccg ccgaaatact cgaagaagaa gccaagcgcg
     2701 ccaacatgtt tttcgtgaac cagcgctggc tgggtgggat gttgaccaac ttccaaacca
     2761 ttcgacgcag catcgacaag atgaagaaga tggagacaac gctcctgaac cccaacgagc
     2821 atggtct
//
LOCUS       DATMTI010000095         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_46155, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000095 DATMTI010000000
VERSION     DATMTI010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46155"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..392
                     /locus_tag="VN039_01645"
     CDS             213..392
                     /locus_tag="VN039_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44718.1"
                     /translation="MKDQIEHVTVISIAKKPAHAQAASLARTALQSMPNGIRTLPRMF
                     KRQVGTVTLAWSKLG"
     gene            complement(503..1732)
                     /locus_tag="VN039_01650"
     CDS             complement(503..1732)
                     /locus_tag="VN039_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HWV44719.1"
                     /translation="MTVEKKRVTPELLDSLLADYKKPEDLIGENGLLKQLTKLLVERA
                     LDAEMAEHLGHGKHETVTNAAGNTRNGKSQKTLKGDFGELPIEIPRDRQGSFEPQLIP
                     KHQTRWAGFDDKILSLYARGMTVREIQSHLQEMYGAEVSPTLISSVTDAVVEEVKAWQ
                     ARPLDALYPIVYLDCIHVKVRDSGSVRAKAVYLAIGINLSGEKEVLGLWISQTEGAKF
                     WLQVVTELKNRGVQDILIACVDGLKGFPEAIETVFPKTTIQLCIVHMVRHSLNYVSWK
                     MRKQVAADLRKIYICATIDEAEQRLTEFEQSWDSAYPPISQSWRRNWSRITPFFDYPP
                     EIRKVIYTTNAIESVNMSLRKITKNRGSFPNDDALIKLFYLALRNISQKWTMPIRDWK
                     AALTRFTIQFEDRMPTR"
     gene            complement(1662..>2013)
                     /locus_tag="VN039_01655"
     CDS             complement(1662..>2013)
                     /locus_tag="VN039_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44720.1"
                     /translation="SLDRKRSQLWMAQAHQITVYDRSGNAVLTLPFQGSKDNNQKTLN
                     IRGLAYDRKQDSIWVNFADRLRRYDSGGCPRFCVNGYQAGNCRAYRSEDDDRREEESD
                     ARTAGQPAGRLQEA"
BASE COUNT          436 a          559 c          543 g          475 t
ORIGIN      
        1 gaaaagaaaa tgtgcgtggc cgcgtgacca atgtaatgaa cttttttggc gcagcaagca
       61 taagcaagtc agtatatcga cgctgcattc cgcaataccc tctagcctaa gcgtgtcaac
      121 gcaccgactc caaacctgcg gatcactaac ctgcgtggct gaatccaaca cggcattggc
      181 agattcgcca atcaccggat tcaacaactc ccatgaaaga tcagatcgaa cacgtcacgg
      241 ttatcagtat cgcgaagaag cctgcccacg cccaggcggc aagccttgcg cggacggccc
      301 tgcaatccat gcccaacggc atccgcacct tgccgagaat gttcaaacga caggtcggca
      361 ccgtcacgct ggcatggagc aagctggggt gagacgcgag atacggcaca gtgaaccaat
      421 aagcaatctg aattttcaca atgtattgct ctgaattcag gactcagagg gtgtcccgat
      481 ttttgtgtaa acggcgtgtg ggttaacggg ttggcatccg gtcttcaaac tggatggtaa
      541 atcgggtcag tgcggctttc cagtcacgga tcggcatggt ccatttctgg ctgatatttc
      601 gcagggccag gtagaacagt ttgatcagcg catcatcgtt cggaaacgag cctcgatttt
      661 tggtgatctt gcgcaggctc atgtttaccg actcaatggc gttggtggtg tagatcactt
      721 tgcggatctc tggtgggtag tcaaagaacg gcgtgatacg cgaccagtta cggcgccagg
      781 attggctgat gggtggatac gcactatccc agctctgctc aaattcggtg agtcgctgtt
      841 cggcttcatc gatcgtggca cagatgtaga ttttcctgag gtcggccgcc acctgtttgc
      901 gcatcttcca gctgacgtag ttcaggctgt gacgcaccat gtgcacgata cagagttgaa
      961 tggtggtctt cggaaacacc gtctcaatgg cctccggaaa gcctttcagg ccatcgacgc
     1021 aggcgatgag aatatcttgc acaccacgat tcttgagctc ggtcaccacc tgcagccaga
     1081 atttggcgcc ttccgtttga gaaatccaca gacctagaac ttctttctcg ccggatagat
     1141 tgatcccgat ggccaggtac acggccttgg cccggacgct gccagaatcg cgtaccttca
     1201 catggatgca gtccaggtaa acgatcgggt acagcgcatc cagcggccga gcttgccatg
     1261 ccttaacttc ttcaaccacg gcatcggtga ctgacgagat caacgtcggc gagacctcgg
     1321 cgccgtacat ctcctgcagg tgcgactgaa tctcacgcac ggtcatgccg cgtgcgtaca
     1381 gcgacaggat cttgtcgtcg aatccggccc agcgggtttg gtgtttgggg atcagttgcg
     1441 gctcgaagct gccctgacgg tctcggggaa tctcaatggg aagttcgcca aaatcaccct
     1501 tgagggtttt ctggctcttg ccgttgcggg tattaccggc ggcgttggtg acagtctcgt
     1561 gcttgccgtg acccaggtgc tcagccatct cagcatccag tgcccgttcc accagcagct
     1621 tggtcagctg cttgagcaga ccattctcac caatcaggtc ttcaggcttc ttgtaatcgg
     1681 ccagcaggct gtccagcagt tctggcgtca ctctcttctt ctctacggtc atcatcttcg
     1741 ctcctataag cgcggcagtt tcctgcctga taaccgttta cacaaaatct aggacaccct
     1801 cctgagtcat aacggcgcag acgatccgcg aaattgaccc agatgctgtc ctgcttgcgg
     1861 tcataagcca gcccacgaat attcagcgtc ttttggttgt tgtccttact gccttggaaa
     1921 ggcagagtga gaaccgcgtt tccgctgcga tcgtaaacag tgatctggtg ggcttgcgcc
     1981 atccagagct gggaacgctt tcgatccagg ctg
//
LOCUS       DATMTI010000096         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_767512, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000096 DATMTI010000000
VERSION     DATMTI010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767512"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VN039_01660"
     CDS             complement(<1..338)
                     /locus_tag="VN039_01660"
                     /inference="COORDINATES: protein motif:HMM:NF039494.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWV44721.1"
                     /translation="MTDNEIAVLISLNNWGPGPGNSAAAIKFLDTAIAICRAESGGNP
                     NAVSNTGDYGLWQINKAAHPQYFPPKAQGGYIWDPLSNTYVAKTIYAAAGGWSPWSTY
                     KNGSYKAHLG"
     gene            complement(331..1050)
                     /locus_tag="VN039_01665"
     CDS             complement(331..1050)
                     /locus_tag="VN039_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44722.1"
                     /translation="MKSSSSGSATDAPADPADAAATDASGQSSDQPVFSAIPSTSVST
                     GSSDTGSTAAVSTATDQTWANTAIAWLITQGASVSMATNAIQKYLAGETLSLDEGKLR
                     DKAIRQFGAPPESGYVQTSTAGYTGPAVKQGNPPTTHTVKGKADNTPQELARLYYGMD
                     NPDVVQYLIANNPSLVTPYRVGQKVSIPRYHSPKYYKSTQRNNTFGEIARINGTTIAA
                     IRSLNPTLISPVPVGTRVRVD"
     gene            complement(1170..1724)
                     /locus_tag="VN039_01670"
     CDS             complement(1170..1724)
                     /locus_tag="VN039_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44723.1"
                     /translation="MTNAWFLGAQTSIPQSEDEVRNTTEPIRADSPAEDRAAPNWNEF
                     RTDETGQVTGPTAARMLASDTKDSVQYEPWWAQLAQQNHNELIDNQVSSSGTAAAREA
                     AGIQGHGTAQYAIGIEPVIREGAAFGSDYFTAYEAGANPLAGAYMTPVDIDNSGRAAV
                     ASHAQQNARDAYSGLYDAFLNASK"
     gene            complement(1917..2156)
                     /locus_tag="VN039_01675"
     CDS             complement(1917..2156)
                     /locus_tag="VN039_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44724.1"
                     /translation="MSADHLFIGAGSLAFISNFKQAGGFPPNGIATIAGTAVLAFLAA
                     ATKETAIAPAVSAFAALVLLVAVFQYVPGLTKKGK"
     gene            complement(2949..3143)
                     /locus_tag="VN039_01680"
     CDS             complement(2949..3143)
                     /locus_tag="VN039_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44725.1"
                     /translation="MYYVVSKVGDEFVNTTVCESEDVADAIVADRKVSQPFGEHYVSE
                     EYPAEANPADESVSTPGHDK"
     gene            complement(3152..3355)
                     /locus_tag="VN039_01685"
     CDS             complement(3152..3355)
                     /locus_tag="VN039_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44726.1"
                     /translation="MPTATDMGQVSPDLKGSPKNPARDPKSKDKVDKGSIGDRALMDA
                     VMLIVGALALLVLLWYSVRNHNI"
     gene            complement(3359..4522)
                     /locus_tag="VN039_01690"
     CDS             complement(3359..4522)
                     /locus_tag="VN039_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44727.1"
                     /translation="MTTMAPEAAANAAPVSGLPFATASRPQRRFQNVQSNVSNANAGS
                     FQVIQLPATGWVRRVSLFITATATAASAGALVAGDAPFNLIQNITLTDATGQPVIQPI
                     SGFNAYLVKKYFATGSQATNSPSPWENPRMNPEYALSGTSTAFSAVFRIDLDFEIDAR
                     TGYGCIPNLDSNASLQLKVDYAAGTVAYTGTGLTAQTISMRTEVEYWAPVGNSIGGVP
                     VQTAPPGAGDYAEVRYETQPVSASSENLVTATNRGGLVRGVLLVSRAAGVRTAITAAS
                     NLGILLDNQPINEGIPIESHYHQIRQMYGYIGAMNGTSLVPLTGGTMDGLDTGVVPIN
                     FGALSLGRDSWLNTRAGSLFQFKVTPGASATTLELVTILAQVKDATAFYNSGA"
     gene            complement(4696..>5047)
                     /locus_tag="VN039_01695"
     CDS             complement(4696..>5047)
                     /locus_tag="VN039_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44728.1"
                     /translation="GKWTLHVDEGLYVCHPSYLGLSDELAMSHALGRSSGLTLITLAQ
                     RPANLPLILYGSASHAFVGRTRQASDNKRLSQLGGRHSARELGDRIAGQGRHDFLWVP
                     VAPDWEPETMNLRY"
BASE COUNT         1320 a         1381 c         1390 g          956 t
ORIGIN      
        1 tgccctagat gagctttgta agagccattc ttataggtgc tccacgggga ccagccaccg
       61 gcagcggcat aaatagtctt agcgacgtag gtattactca gcgggtccca aatataaccg
      121 ccctgagctt tgggagggaa atactgaggg tgtgcagcct tgttgatttg ccagagtcca
      181 tagtcaccag tattagaaac cgcattaggg tttccaccag actcggcacg gcagatagcg
      241 atagcggtat caagaaactt gatagcggca gcgctattgc cgggtccggg accccaattg
      301 ttgagagaga ttagtaccgc aatctcgttg tcagtcaacg cggacacggg tacctaccgg
      361 aacgggagaa ataagcgtcg ggttaagcga acgaatagcc gcgattgtcg taccgttgat
      421 acgggcaatc tctccgaacg tgttgttacg ctgagttgac ttgtaatact tcggcgaatg
      481 gtagcgcgga atactgacct tttgcccaac acgatacggc gtaaccagag aggggttatt
      541 tgcaatgaga tactgtacaa catcagggtt atccataccg tagtacagcc tagccaactc
      601 ctgaggagtg ttgtcagcct tgcccttcac agtatgcgta gtcggcggat tcccttgctt
      661 cacagcagga ccggtataac cagcggtaga ggtctggaca tagccagact ccgggggagc
      721 gccaaactgc cgaatagcct tatccctgag cttaccctca tccagcgaaa gagtctcgcc
      781 agcaagatac ttctgaatag cattagtcgc catgctaact gatgcgccct gagtgataag
      841 ccatgcaata gccgtgttcg cccaagtctg gtccgtagcc gtggagacgg cagcggtaga
      901 gccagtatca ctagagcccg tgctgaccga ggtagacggg atagcagaga atacgggctg
      961 atccgacgac tgccccgatg catcggtggc ggcagcgtcg gcgggatcgg cgggggcatc
     1021 cgtggcggaa ccgctgctag agcttttcat acgaatagcc atatacagga gtcccgcgac
     1081 aaagataagc gcgaggatgg aaaccggcac accggcaacc ttacgcttaa ggagagagcc
     1141 aacctgaggc ataggcgttt cactcatcgt cacttactcg cattcaggaa agcgtcatag
     1201 agcccggaat aggcatcgcg tgcgttctgc tgtgcatgac tggcgacagc agcgcgcccc
     1261 gagttatcaa tatccacggg agtcatgtac gcgccagcaa gagggttagc gccagcctca
     1321 tacgcggtaa agtagtcgga accgaatgcc gcgccctcac gaataaccgg ctcaataccg
     1381 atagcatact gagcagtacc gtgaccctga ataccggcag cctcacgagc ggcagcggta
     1441 ccggacgacg atacctgatt gtcaatcagc tcgttatggt tctgctgcgc aagctgagcc
     1501 caccacggct catactggac ggaatcctta gtgtcggatg ccagcatacg agcagccgta
     1561 ggaccggtaa cctgtccagt ctcatcggtg cggaattcgt tccagtttgg cgcggcacga
     1621 tcctcggcgg gagagtcggc gcggataggc tcagtcgtat tgcgtacctc atcctcagac
     1681 tgaggaatag aggtctgcgc ccctagaaac catgcattag tcatcgtcga tacctccgat
     1741 gatagccgcg aaaatggaac attacttaag cggggagaca gccgcgcgct gcaagcctgc
     1801 aataccgttg agcagtgccg taaggacacg agcagtatcc gagcgcttag acacaacgat
     1861 agcgagagcg gttacagtga acacaaccga aacaacgccg ataaccttat ccatgattac
     1921 ttacccttct tagtgagtcc ggggacgtat tgaaaaacag cgaccaatag aacgagagca
     1981 gcgaacgcgg atacagccgg agcaattgct gtctccttag tcgcggcagc aaggaaagcc
     2041 agcacagcag taccggcaat cgtagcgatt ccattaggag ggaaaccgcc agcttgctta
     2101 aagttggaaa taaacgcgag acttcccgcg ccgataaaca ggtgatccgc gctcatttac
     2161 tctccccttg caccgggcgc aattggtgac gatacggact cattgagccg ccagtattca
     2221 ggagtagatc cgaaatactt aacgtctgcg gaaagggaac cggagccgat ccccttggaa
     2281 acggaacgag cggaatacat ggaatcgcta ccgtcaacaa tacccatgtg cttagcgggc
     2341 caaataacga tatccccaac ctgaggactc ttgacctgag tagcaaaccg cgtagcgata
     2401 gagtcaaacg aacgagtggt aaaccgagga cccttgtaaa gacccgtggt gtacatcgcg
     2461 cgccatacca gtccggaaca gtcatagtaa ttgggaccag tcgcgccgag ctgataaccc
     2521 ttagcattat tgcctagggt tttcatggta ctaaggaggg gagtagacga gtccgacgca
     2581 cctgcaaggc ccgccaatga acctggaccg gtagccttgg ttccggtcag gtcgccggac
     2641 ccgtctacat tattcccgcg ctgactaagg acattagcag cggcagacgt atccccattc
     2701 actagggcaa ggaaaagatc cttaatgtcc tgcggagttt cggcaaccct accgacaata
     2761 acctgtcgaa taaggagcgt ggctccgagg accaatacgt aaccgactgc ggtatccatt
     2821 agctttcgcg tgcccccacc ctagtcagaa cacgcggcgc aggaaaccaa ggaggatgag
     2881 cccgacgatg atagcaacca gaatacggtt gttaagagcg tcaataatct tacccattat
     2941 cttatccttt acttgtcgtg accgggagtc gaaaccgact cgtcagcggg gttagcctcg
     3001 gcggggtatt cctccgaaac gtagtgctca ccgaaaggct gagacacctt acgatccgcg
     3061 acgattgcat cagcaacatc ctcggactcg caaacagtag tgttaacgaa ctcgtcgcca
     3121 accttggaaa caacgtagta catgctattc cttagatgtt gtgattgcgg acggaatacc
     3181 agagcagaac gagcagagcc agagcgccga caataagcat gacggcatcc atcagagccc
     3241 gatccccgat agagcccttg tctaccttgt ccttggactt agggtcacgg gcagggttct
     3301 taggagagcc cttaaggtca ggcgaaacct gtcccatatc ggtagccgta ggcatcggtc
     3361 acgcccccga gttatagaac gcggtagcgt ccttcacctg agccagaatg gtaaccagct
     3421 caagcgtagt tgcagacgca cccggagtaa ccttaaactg gaacagcgaa ccggcacgcg
     3481 tgttaagcca cgaatcacgg ccgagagaca gagcaccgaa gttaatcgga acaacgcccg
     3541 tgtcgagtcc gtccatagta ccgcccgtaa gcgggaccag agacgtacca ttcatagcgc
     3601 cgatgtaacc gtacatctgg cggatctggt ggtagtgcga ctcaatcgga ataccctcgt
     3661 tgatcggctg gttatccagc agaataccga ggttagacgc ggcggtaata gccgtacgaa
     3721 caccggcagc acgcgacacg agcagaacgc cgcgaaccag accgcctcgg ttagtggcag
     3781 taacaaggtt ctcagacgac gccgaaacgg gctgagtctc gtaacggacc tcggcatagt
     3841 cgccagcgcc cggaggggca gtctggaccg gaacgccgcc gatagagttc ccgacaggcg
     3901 cccaatactc aacctcagta cgcatggaaa tagtctgagc agtcagaccg gtacccgtgt
     3961 aggcaaccgt accagccgca tagtcaacct taagctgaag cgaagcattc gagtcaaggt
     4021 taggaatgca gccgtaaccg gtacgcgcat caatctcgaa atcaaggtcg atacggaaca
     4081 cggcagagaa cgcggtagag gtacccgaca gagcatactc cgggttcata cgcgggttct
     4141 cccacggaga cggggagtta gtagcctgcg aaccagtcgc aaaatacttc ttcaccagat
     4201 acgcgttaaa cccactaata ggctgaatca cgggctgacc cgtggcatcc gtcagagtga
     4261 tgttctgaat caggttaaac ggggcatcgc cagcaaccag agcaccggca gaggcagcag
     4321 tcgcggtagc cgtaataaac agcgacacgc gacgaaccca accggtagcc ggaagctgga
     4381 tcacctgaaa tgagccagcg ttagcgttag aaacgttact ctggacattc tggaatcgac
     4441 gctgcgggcg gctagccgta gcgaacggaa gcccggagac gggagccgca ttagcggcag
     4501 cctcaggagc cattgtagtc atacgatcat acctctctat tatctccggg atttagaaac
     4561 cggtagcaat gaaagcacgg gcaaggcgtc cgtccgagga cagagccagc aagtgcaggg
     4621 tgccaaacaa cgcgaataca accaagcatg tagcaaggaa gtgaaccgcg ctaatatgag
     4681 tgtgcggatt cattctcaat acctcagatt catagtttcg ggttcccaat ccggtgctac
     4741 aggcacccat aggaaatcat gcctaccctg tccggcgatt ctatcaccca attcccgtgc
     4801 agaatgccga ccgcccaact ggctgagcct cttgttgtcg ctcgcctgcc gcgtccggcc
     4861 cacaaaggca tgcgaggccg agccgtaaag aataagcggc agattagcgg ggcgctgtgc
     4921 aagggtaata agcgtcaatc ccgaggaacg tccaagcgca tgagacatag ccaattcgtc
     4981 agaaaggccg agatatgaag ggtgacatac atacagccct tcatcaacat ggagagtcca
     5041 tttgccg
//
LOCUS       DATMTI010000097         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_513017, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000097 DATMTI010000000
VERSION     DATMTI010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513017"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /gene="glyS"
                     /locus_tag="VN039_01700"
     CDS             <1..999
                     /gene="glyS"
                     /locus_tag="VN039_01700"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWV44729.1"
                     /translation="IAAQLSSGQDELQQICERAAALSKADLVTGIVGEFPELQGIMGG
                     EYALHDGESPAVAQAIREQYLPKAIEGELPKMLAGQILSLADRLDTIAAFFHVGIVPT
                     GSEDPFALRRHATAIVRIVIEGNLRINFGTCIDHAMGIVTKDGFKGASGLELEGRRRI
                     TEFIFERVRHYGRIMYALRDDVIESVLKPVHDTVIDLVDLIRKMKALEAAMRKPEFDS
                     LIVGFKRAHRLVEKEQWERRPVDAAMFQHPSEPALHKAVENERGRIEDSVKTGEYSRA
                     LNALVGLKPSVDAFFEAVMVNAEDKAIRSNRLSLLKEVDELFMSFADFSQIVVQGR"
     gene            1062..1553
                     /locus_tag="VN039_01705"
     CDS             1062..1553
                     /locus_tag="VN039_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44730.1"
                     /translation="MDQPMTDSRRRADKIRRFSLGVSLALMVLCNAVLLGGLALSGIN
                     LDDLVKTPDFFNAKQDVCLRLTWQSVPGANEPVRLCSEWLNLSDPSGKAHYLQPDTKL
                     KKGLDGQYYVDQGVQADYRLLMLMLFVTAVIIGGIRAKWFLVNRYRLRLESTDGRRAS
                     PAH"
     gene            1583..>2211
                     /locus_tag="VN039_01710"
     CDS             1583..>2211
                     /locus_tag="VN039_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWV44731.1"
                     /translation="MAKKYVYYFGDGKAEGTSNMKELLGGKGAGLAEMTNLGISVPPG
                     FTISTEACVEYYRIGKQYPHGMWDATLQALKRVERSMGMGFGNPERPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVEGLAAKTRNDRFAQDSYRRFVSMFGSIVMGVPREHFEAI
                     LKHKKTEVGVAHETHLDARHLRELVASFKALIKEETKKDFPDEPLEQLRL"
BASE COUNT          494 a          557 c          679 g          481 t
ORIGIN      
        1 attgcagcgc agttgtcttc cgggcaggat gaattgcaac agatttgtga gcgggcggcg
       61 gctttgtcca aagcagatct cgtcaccggc atcgtcggcg agtttccgga gttacagggc
      121 attatgggcg gcgaatacgc cctgcatgat ggagaatcgc ctgcggtggc ccaggccatt
      181 cgagaacagt atctgcccaa ggcgattgaa ggagagttgc ctaaaatgtt ggcgggacag
      241 attctttccc tggccgatcg gctggatacc attgcggcgt ttttccatgt cggtatcgtc
      301 ccgacgggat cagaagatcc ttttgcattg cgccgtcacg ccaccgcaat cgtgaggatc
      361 gtgattgagg ggaaccttcg aatcaatttc ggaacatgta tcgatcacgc tatgggcatt
      421 gtgacgaagg acggattcaa aggcgcatcg ggattggaac tagaaggacg tcggcgcatc
      481 acggagttca tctttgagcg ggttcgacac tacggtcgta ttatgtacgc cttgcgagac
      541 gacgtcatcg agtcggtatt gaagccggtt catgacacgg tgattgacct tgttgatctt
      601 atccggaaaa tgaaggccct tgaagcagcg atgcgaaagc cggagttcga ttccttgatc
      661 gtcggtttca aacgggcgca tcggttggtg gaaaaagaac agtgggaacg ccggccggtc
      721 gatgccgcca tgtttcagca tccgtcagag ccggcgcttc acaaagccgt agagaatgaa
      781 cgcggccgga tagaggattc ggttaaaacc ggtgagtaca gccgagcatt gaatgcgctg
      841 gtgggtctta agccgtccgt tgacgccttt ttcgaggctg tgatggtcaa tgccgaggac
      901 aaggccatcc gcagtaaccg attgtcgctg ctcaaagaag tcgatgagtt attcatgtca
      961 ttcgcggact tttcccagat tgtggtacaa ggacggtagg tcaaatcgga cgaagagaca
     1021 ggcatcctgc cggcgagatt tgaggcaaga ctggaggtgc catggatcag ccgatgacgg
     1081 acagccgacg tcgggcggac aagatccggc gattttctct gggagtctcg ctggccttga
     1141 tggtgctctg caatgccgtt cttctcggcg ggctcgcgct cagcggcatt aatctggacg
     1201 acttggtcaa gacgccggat ttcttcaatg ccaagcagga cgtgtgcctt cgactcacgt
     1261 ggcagtcagt gcccggagcg aatgaaccgg ttcgactctg ttcggaatgg ctgaatctct
     1321 ccgacccgag tggaaaggct cattatctcc agcccgacac gaagctcaag aaggggcttg
     1381 acggccagta ctacgtcgat caaggtgttc aggctgacta tcgattattg atgctgatgc
     1441 tttttgtcac cgcggtgatc atcggtggca taagggcgaa gtggttcttg gtgaaccgct
     1501 atcggctccg tctggaatca accgacggtc gtcgcgcatc gcccgctcat tgatacgcac
     1561 tcttcccgga aggagacggc acgtggcaaa gaaatatgtc tactatttcg gcgacggcaa
     1621 ggccgagggc acgtcgaaca tgaaggaact gctcggcgga aaaggcgcag ggttggccga
     1681 gatgaccaat ttgggtatct ccgtcccgcc cggatttacg atttccaccg aagcttgcgt
     1741 cgagtattac aggatcggca agcaataccc tcacggaatg tgggacgcca cattgcaggc
     1801 gctcaaacgc gtggagcgtt cgatggggat gggattcggc aatcccgaac ggccgttgct
     1861 ggtctcggtc cgatccggtg cgcgtgcctc gatgcccggc atgatggaca ccgtgttgaa
     1921 cgtgggtctc acgaccaaga cggtcgaggg cctcgcggcc aagacccgca atgatcgctt
     1981 cgcccaggac agctatcgac gatttgtgtc gatgttcggc agcattgtga tgggcgtgcc
     2041 gcgcgagcat tttgaagcga tcctcaagca caagaagaca gaagtcggcg tggcgcacga
     2101 gacgcatttg gatgccaggc acctccgcga attagtggcg agtttcaaag ccctcatcaa
     2161 agaagaaacg aaaaaagact ttccggatga accgctggaa cagctgcggc t
//
LOCUS       DATMTI010000098         1731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_216160, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000098 DATMTI010000000
VERSION     DATMTI010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216160"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VN039_01715"
     CDS             <1..590
                     /locus_tag="VN039_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44732.1"
                     /translation="QVVKQEALLSSLLDGNKVMQQQIDLLNQELRDAKSTTEATKTEA
                     KQQIEQLELQLAEAKRMMATQMTESLKVEEKGAQSDTLPRPMPTVAKVVEDALARNGY
                     QMKVSVKTDQRAVYVTERKISNPTTLEVTGSRNQYLISLQALPDSLTKVGVKAEFERV
                     AQGGRILSVSREETSEIEHRLINEINKALANPAKT"
     gene            665..>1731
                     /locus_tag="VN039_01720"
     CDS             665..>1731
                     /locus_tag="VN039_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV44733.1"
                     /translation="MALTTSVHDLRTLIRSSHPLVVIETVEEERVLALLQSVTAQERM
                     PLFEWSVTRGLTRADEGATLSKMTATPLAVLQHLHGLTVEAVFWLKDLGPHLQDPAVC
                     RQLREVAAVYGRSRATCLLTGQPITLPMDLDKIAVRLDLKLPDRDELRSMLRGLLQSL
                     GPRTTTRPRSTTLAQSILSSINDTKSVANRPSSQESDAILRALQGLTLHQARQVIAQC
                     IVERGTLSAEDVQTILKRKVQAIKDGGLLEYYPLEDNRFELGGFTNLKSWLDRAQVGF
                     TAEAKSLNLTPPRGIMLVGVPGCGKSLAAKAIAREWKLPLLKLDAGRLFDKFVGESEK
                     NFRKAIEMAESLSPIVLWIDE"
BASE COUNT          423 a          512 c          460 g          336 t
ORIGIN      
        1 aacaagtcgt gaagcaagaa gctctcctaa gctccttact ggacggaaat aaggtgatgc
       61 agcagcagat cgatctgctc aatcaagaac ttcgggacgc aaagagcacc acggaagcca
      121 cgaagacgga agccaaacag cagatcgagc aactggaatt gcagctggct gaggccaaac
      181 ggatgatggc aacccagatg acggaatccc tcaaagtcga ggagaaaggc gcgcagtccg
      241 atacgctccc tcggcctatg ccgaccgtag ccaaggttgt tgaagacgct ctggcacgca
      301 acggttatca gatgaaggtc agcgtcaaga ccgatcaacg ggcggtctat gtgacggagc
      361 gcaagatctc caacccgacc acgctcgaag taacgggctc aaggaatcaa tacctcattt
      421 cccttcaagc tcttccggac agcctgacga aagtcggcgt aaaagctgaa ttcgaacggg
      481 tcgcacaggg gggccgcatc ttaagcgtca gccgcgaaga aaccagcgaa atcgagcacc
      541 gcctgatcaa cgaaatcaac aaggcgctcg ccaatccagc caagacataa atcttcaaca
      601 cgcgacggtc acaatcgccg gttttcttgg tccggtcgaa gcctcgtgct agcatttccc
      661 tccgatggct ctgacgacca gcgtgcacga tctccgcacc ttgatccgct cctcccatcc
      721 tcttgtcgtc attgaaacgg tagaggagga gcgagttctg gccttactac aatcggtgac
      781 cgcacaggag cgcatgccgc tcttcgagtg gtccgtcacc agaggactca cccgcgccga
      841 cgaaggcgct accctcagta agatgaccgc gacccccttg gctgtccttc aacatcttca
      901 tggcctgacg gtagaagcgg tgttttggct caaggatctt gggccacatc tgcaagatcc
      961 cgctgtctgc cgacaacttc gggaggttgc cgcagtctac ggccgatcac gggccacctg
     1021 cctgttgacc ggtcagccga tcacgctgcc gatggacctc gataaaattg ccgtgcgact
     1081 cgatctgaaa ctgccggatc gagacgagct gcgatcgatg ttgcgcggct tgctccaatc
     1141 gttgggacct cgcacaacta ctcgtccacg atccacgact ctcgcgcaaa gcattctcag
     1201 ttcgataaac gacaccaagt cagtcgcgaa ccgaccctcc tcccaggagt ccgacgcaat
     1261 cctccgtgcc ctacagggct tgacgctcca tcaagctcgc caggtcatcg cacaatgcat
     1321 cgtggaacgc ggcacgctct ctgccgagga tgtccaaaca atcttgaagc gtaaagtgca
     1381 ggcaatcaaa gacggcggcc tcttggaata ctatcccttg gaggataatc ggtttgagtt
     1441 gggaggtttc accaatctga agtcctggct ggatcgggca caagtcgggt ttaccgccga
     1501 agccaaatca ctcaatctca ctccgcctcg cggcatcatg ttagtcggcg tgccgggctg
     1561 cggaaaatcg ctcgcggcga aggccattgc gcgggaatgg aaactcccgc tcctgaaact
     1621 cgatgccggc cggttattcg ataagtttgt cggcgaatcg gaaaagaatt ttcgcaaagc
     1681 gattgagatg gcggaatcct tatccccgat tgttctctgg atcgatgaaa t
//
LOCUS       DATMTI010000099         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_88547, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000099 DATMTI010000000
VERSION     DATMTI010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88547"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="VN039_01725"
     CDS             complement(<1..1554)
                     /locus_tag="VN039_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWV44734.1"
                     /translation="MTENPTSSPPGTRWGLKTKVILSMLLVGLIPLIVGLGMAFWQGS
                     QEIREISGESFEALATEAARKLDLLLAEEIAHTALIANDPMLIRELEHRRDGRGDAKS
                     PTKAPSDLERRWMARDRMAIKSIMENPFSTLLHEYFTGIHSAPGQLLPHVVRSSTKML
                     FLTDAQGMLVATMTEHPPFRHNETRWWQGAFNKAVGRLYIEDVRFDDQVNTYVFTISL
                     PVMDSLRYEVVGVLHRVIDAKEFFSPSTHVIRFGKTGHVMLIDTNGIVISCPLLPTGV
                     PLSDRSLIPLVTPQHPGWVQAPSDGHGGQDTSVIGFSPLHETSRATNGSNGTGSWHTF
                     VWQSSDELFAPIQHLFTWVTVFGAVALVLLASLGYIAAGRIVTPVRQLQQAAQAIGRG
                     ELRTPIQINTGDELEDLAEEFNRMNSQLKAAFAGLADQVKLKTQEVQVLQQSTDQILD
                     AVPTPILLIDAHDVVRYINQAARESFKVQEPMTGRPLFTILPLDPAVQKQLQAEFHTN
                     GNPSLTTDTI"
     gene            complement(1551..>2143)
                     /locus_tag="VN039_01730"
     CDS             complement(1551..>2143)
                     /locus_tag="VN039_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWV44735.1"
                     /translation="ETDLDAVHLQARDIIDAVKRTTAICRDLTSYSRRTSLRQDCLVN
                     INGKLDEALKIARYAVALQDIDIRKLYQPDVVVKGNPDELLHVFVNLITNAVHAMEHH
                     GTLTLETSAVAGTIQIRVSDTGCGIAPEVQSRIFEPFFTTKPPGKGTGLGLYNIKNVI
                     HRMNGSIGVDSHIGRGSTFTLIFHDESGAGERTSQL"
BASE COUNT          443 a          544 c          662 g          494 t
ORIGIN      
        1 gatcgtgtcc gtggtgagag atggattccc gttggtgtga aattccgctt gaagttgttt
       61 ctgcacggcc gggtcgagtg gaagaattgt gaacaacggc cttcccgtca tcggttcctg
      121 gaccttgaag gattcgcggg ccgcctgatt gatgtagcgc acgacatcat gggcatcgat
      181 caaaagaatg ggagtgggaa cggcgtccaa gatctgatct gtggactgct ggaggacctg
      241 gacctcctgg gttttcaatt tgacctgatc tgccagaccg gcgaacgccg ctttcagttg
      301 actgttcatc cgattaaact cttccgccaa gtcttccaat tcgtccccgg tgttgatctg
      361 aatgggtgtc cgcagttcac cgcgtccaat ggcctgcgca gcctgttgca gttgtcgcac
      421 gggtgtcacg atgcggccgg cggcgatgta tcccagggag gccagcagga ccaaggcgac
      481 agctccgaac accgtgaccc aggtgaacag atgctgaatc ggcgcgaaga gttcatcgga
      541 cgattgccat acgaacgtgt gccacgatcc cgtgccatta gagccgttgg tcgctcggct
      601 ggtttcgtgg agaggggaga agccgatcac ggaggtatcc tgccctccat gaccgtcgct
      661 cggggcttgc acccacccgg gatgctgcgg cgtgacgagc gggattaaac tccggtcgga
      721 caaggggaca ccggtcggaa gaagggggca gctgatgacg atgccgttgg tatcgatcaa
      781 catgacatga ccggttttcc cgaatcgaat gacgtgggtc gaaggagaaa agaattcttt
      841 ggcgtcgatc acgcggtgaa gtaccccgac gacttcataa cggaggctgt ccatgacggg
      901 aagtgaaatg gtgaacacat aggtgttcac ctggtcgtcg aaacggacat cttcgatata
      961 gagcctgcct accgctttgt tgaaggcccc ttgccaccat cgggtctcgt tgtggcgaaa
     1021 agggggatgt tcggtcatgg tcgcgacgag catgccctgt gcatcggtca agaacagcat
     1081 cttcgtcgag gagcgaacca cgtgcggaag caattgtccc ggtgcgctat ggatgccggt
     1141 aaagtactcg tggagcaagg tgctgaacgg gttctccatg atggatttga tcgccatccg
     1201 gtctcgtgcc atccaacgcc gttctaagtc cgagggggct ttcgtgggac tcttggcatc
     1261 accccgtcca tcacgccgat gttccagttc gcggatgagc atcgggtcat tcgcgatgag
     1321 cgccgtgtgg gcgatttcct cggcgagcag gagatcgagt ttgcgggcgg cttcagtcgc
     1381 cagcgcttcg aagctttccc cgctgatttc ccggatttct tgggaccctt gccagaatgc
     1441 catacccaag ccgacgatga gcgggatgag accgacgagc agcatggaga ggatgacttt
     1501 ggtcttgagt ccccatctgg tgccgggcgg cgaggatgtt ggattttcgg tcataattga
     1561 gatgttcttt caccggcccc ggactcatca tgaaagatga gcgtgaaggt tgaaccacgg
     1621 ccaatgtggc tgtcgacccc gatggagcca ttcatgcgat ggatgacatt tttaatgttg
     1681 tataagccca atcccgttcc tttccccggt ggcttcgtgg tgaaaaaggg ttcgaagatc
     1741 cgactttgca cttccggggc aatgccgcag ccggtgtcag aaacacgaat ctgtattgtg
     1801 ccagccaccg ctgaggtttc cagcgtcaag gtgccgtggt gttccatggc atggacggca
     1861 ttcgtgatga gattgacgaa cacatgaagg agttcatcgg ggtttccctt cacgacgacg
     1921 tcaggttgat agagtttgcg aatgtcaatg tcttgaagcg ccacggcata gcgagcgatc
     1981 ttgagggctt catccagctt gccgttgatg ttgaccaggc agtcttgccg taaggatgtg
     2041 cggcgtgagt aagaagtgag atctcgacaa atggcggttg tgcgtttgac cgcatcgata
     2101 atgtcccggg cttgaagatg tacggcatcc aagtccgttt cat
//
LOCUS       DATMTI010000100         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_428060, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000100 DATMTI010000000
VERSION     DATMTI010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428060"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VN039_01735"
     CDS             <1..424
                     /locus_tag="VN039_01735"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HWV44736.1"
                     /translation="TGFSRIASEVAKSKIANFKSRETELKRMKEMVDEAIQILQHTRT
                     RIEEVGRLLHQSWLCKRSLSGQVSTPEIDHLYEEAMRGGALGGKILGAGGGGFLLLFV
                     KPELQSKVHERLKHLIHVPFRFENTGSRVVLYQPHGLS"
     gene            430..1392
                     /locus_tag="VN039_01740"
     CDS             430..1392
                     /locus_tag="VN039_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HWV44737.1"
                     /translation="MLTHDASIYVAGHAGLIGSAVVRRLERDGYRMLITRRRSELELQ
                     NSRQVSRFFEEVHPEYVILAAGRVGGIMENQTFPADFMDENMAVQLNVLKAARKVGVR
                     RLIFFGSSCMYPKNCSQPMAEDALLSGKPEPTSLPYAISKLAGTYLCLAYNKQDHDTR
                     FIPVIPNSAYGPHDNFDPKSAHVLSALMARFHQAKVSGAESVVLWGSGSPRREFIHAS
                     DIADACVHLLNQADLAVEFPLNIGVDQDVSIKELAELIAEVVGYKGGVEWDRTKPDGA
                     PRKLLDSERIRSLGWKPTVTLAEGIAATYRWYLNHVDRETSQVS"
     gene            1412..2011
                     /locus_tag="VN039_01745"
     CDS             1412..2011
                     /locus_tag="VN039_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWV44738.1"
                     /translation="MTKEELISFEEEIAALFNAGKIRAPIHLYYGNEEAIIRVFRNIR
                     VQDWVFCSWRSHYQCLLKGVPKDRVRDEILAGRSISLCFPEQRVFSSAIVGGVLPIAV
                     GAAMSIRQRGEDAKVYCFMGDMTAETGVAHEAIKYSRNHRLPIHFIVEDNAKSVCTDT
                     REVWNQPRLSFEDAADEYISYYRYETKYPHAGAGVRVQF"
     gene            2020..>2532
                     /locus_tag="VN039_01750"
     CDS             2020..>2532
                     /locus_tag="VN039_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019112131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44739.1"
                     /translation="MKYFDELKRSMNFLAQDSRTVFLGQAVSVAGTAMSNTLKEVPSD
                     RLIELPVAEEMQMGMTTGFALTGLVPVSIFPRWNFLLLAINQLVNHLDKIQVMSNGGY
                     KTKAIIRTGIGSQRPLHPQHQHVGDFTEAIRMMCSTIEVIRLEEPKDVFPAYEWALLR
                     DDGRSTIVVEY"
BASE COUNT          584 a          625 c          749 g          574 t
ORIGIN      
        1 cacaggattt tccagaattg cgtccgaagt cgccaagtcc aagatcgcga actttaagag
       61 ccgggaaacc gagttgaaac gcatgaagga gatggtcgat gaagccatcc agatcctcca
      121 gcatacgcgg acacggatcg aggaagtggg ccgattgctt caccaaagct ggctctgtaa
      181 gcgcagcctt tcaggccagg tttcgacgcc ggagatcgac catctgtatg aggaagccat
      241 gaggggaggc gctctgggag ggaagatcct tggcgccggc ggcgggggat ttctgttgtt
      301 gtttgtgaag cctgagctgc aatcgaaagt gcacgaacgg ttgaagcatc tgatacatgt
      361 gccgttcagg tttgaaaata caggtagtcg agtcgtgctg taccaaccgc atgggctctc
      421 ctgaacgcga tgctgacgca tgatgcctcc atatatgtcg caggccatgc cggtcttatc
      481 ggctcggcgg tcgttcgacg gttggagcgg gatggatacc gcatgctgat caccagacgg
      541 cgcagcgaat tggagcttca gaattcccga caggtctcca ggttcttcga ggaggtccat
      601 ccggagtatg tgattctcgc tgccggacga gtcggcggaa taatggaaaa tcaaacattt
      661 cccgccgact ttatggacga gaatatggcc gttcagctca atgtgctgaa ggcagcacgt
      721 aaggtcggcg tgagaagact gattttcttc ggctcttcct gcatgtaccc caagaactgt
      781 tctcagccga tggccgagga tgcgcttttg tcgggcaagc cggaaccgac gagcctgccg
      841 tatgcgattt ccaaactcgc aggaacgtat ttgtgtttgg cgtataacaa gcaagaccac
      901 gatacccgtt tcattccggt gattccgaat agcgcctatg ggccgcacga caattttgac
      961 cccaaatcag cccatgtctt atccgcgctc atggcacgtt ttcatcaggc aaaggtgagc
     1021 ggcgccgaat cggtcgtctt gtggggaagc ggctcgccga gacgggagtt catccatgca
     1081 tccgatattg cggatgcgtg cgtgcatctc ttgaatcaag ccgatctcgc tgtcgaattc
     1141 cctctcaaca ttggagtgga tcaggatgta tcaattaaag aactggctga attgatcgcc
     1201 gaagtggtgg ggtacaaggg aggagtggaa tgggaccgga caaagcccga tggcgcgcca
     1261 cgaaagctgc tcgatagcga acgaatccgt tctctgggat ggaagccaac ggttacgctt
     1321 gccgaaggga ttgccgccac ctaccggtgg tacctcaacc atgttgacag agagacctca
     1381 caggtctcat agcgttgcaa aggatgggaa gatgacgaaa gaagagttga tttcgtttga
     1441 agaagaaatt gcggccttgt tcaacgcggg aaagattcgc gcgcccattc atctgtatta
     1501 tggaaatgag gaggcgatca tccgagtgtt tcgcaacatt cgtgttcaag actgggtgtt
     1561 ttgctcgtgg cggtcccatt atcagtgctt gctcaagggg gtgcccaaag atcgtgtccg
     1621 agatgaaatt ttggccggac gttcgatctc actctgtttt cccgaacagc gtgtgttctc
     1681 ctcggccatc gtcggtggtg tcttgccgat cgccgtcggt gcggcgatgt cgatccggca
     1741 gcgtggagaa gatgcgaaag tctactgttt catgggcgac atgacggcgg aaacgggggt
     1801 tgcgcacgaa gcgatcaaat acagccggaa ccatcgcttg ccgatccact tcatcgtcga
     1861 agacaacgcc aagtcggtct gtaccgatac cagggaggtt tggaatcagc ctcgtttgag
     1921 ctttgaggac gcagccgatg agtacatcag ctactaccgc tacgaaacga agtatccgca
     1981 tgcaggtgcc ggcgtgcggg ttcaattcta aggtgagaca tgaaatattt tgatgaactc
     2041 aagcggtcca tgaatttcct tgcacaagac tcgcggacgg tctttctcgg acaagcggtg
     2101 tccgttgccg gtacggcgat gagcaacacg ctcaaggagg taccgtcgga tcgcttgatc
     2161 gagcttccgg tcgcggagga aatgcagatg ggcatgacga ccggctttgc gttgaccggg
     2221 cttgttccag tgagcatttt cccgcgatgg aactttttgc tcttggcgat caatcaattg
     2281 gtcaatcacc tggataaaat tcaggtaatg tccaacggtg ggtacaagac caaggctatc
     2341 atccggacgg gaatcgggtc tcaacgcccg ctccaccctc agcatcaaca tgtgggtgac
     2401 tttacggaag cgatccgcat gatgtgcagc acgattgaag tcattcggtt ggaagaaccg
     2461 aaagacgtgt tccccgccta tgagtgggcg ctgttgcgtg acgatggacg cagcaccatc
     2521 gtggtcgagt at
//
LOCUS       DATMTI010000101         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_767609, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000101 DATMTI010000000
VERSION     DATMTI010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767609"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VN039_01755"
     CDS             complement(<1..251)
                     /locus_tag="VN039_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44740.1"
                     /translation="MTAHPQTNWFIAAARSAVDIPPQRGQLIPFLLAFGHLIRNGVGY
                     SAQHKVREIMSAERAWAVVNIIASFRRIGLRRSYMWESDG"
     gene            264..848
                     /locus_tag="VN039_01760"
     CDS             264..848
                     /locus_tag="VN039_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV44741.1"
                     /translation="MSLRRDRKFDANVSTAREARQFYRAAGWQRDIGEEKTMIGYGKR
                     LLLIHDPETTQLPLTTILEQEGFVVVQVHGGVQALCEIQFRHFDAVVIGSHIPDLNGL
                     DLLKQSRTTWPEIPIILFVEADWDKCDVAEAIGAFAWIRKSSDLSILLSMLSLAMNQR
                     EERESDQAHHYKIKSGLRHSGNMPARCEGVSEFL"
     gene            862..984
                     /locus_tag="VN039_01765"
     CDS             862..984
                     /locus_tag="VN039_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44742.1"
                     /translation="MPLIFLCCLVMASAVLSFGLMLWFVVLGIVWFVMPGMEEG"
     gene            1161..1709
                     /locus_tag="VN039_01770"
     CDS             1161..1709
                     /locus_tag="VN039_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWV44743.1"
                     /translation="MFQQAEQQREHGEQEARMPAAVRILLAVDDSDNSRRVVQYVGSL
                     LSRTPDAVITLFHVLKPMPRRFLEHGGSENPAVEAQLSMQLRHEQDAWIQKERESECH
                     VLKQACETLTRAGFDMNRVTVKYGHDDDIARNILEEARNGHYDTIAVGRQGISRIKQL
                     FGGGTTDHLVHAAKGVAIWVVA"
     gene            1781..3112
                     /locus_tag="VN039_01775"
     CDS             1781..3112
                     /locus_tag="VN039_01775"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV44744.1"
                     /translation="MSMGKPRVVILGGGFGGMYAALEFERALARGADLDVTLVNRDNF
                     FLFTPMLHEVAASDLDITNIVSPIRKLLRRVTFFHGDIEAIDLVHKRVGLSHGHEQHC
                     HSLPYDHLVLALGSTTNFFNIPGLAERALTMKSLSDAIALRNHLIANLEEADFECGAS
                     LRAPLLNFVVAGGGFAGVETIAAMNDFLREAVRFFPHIREDMLRIILVSAGKVILPEL
                     GEELGTYAQRKLREQKVEIHANCKVTAVTDRDVTLSDGTTVTTNTLVWTAGISPHSLL
                     DTLPCPKMRGRVLVNEYLEVPGWPGLWAFGDCALVPNRKTGEYHPPTAQHALREGRVA
                     ARNILATLRGERMKPFVYSTLGLLAPIGKRTGVATILGVKFSGFIAWWLWRTIYLLKL
                     PRFEKKVLVALDWTLDVLFSKDLVHFRTTRPSTLRPTEGELESTAVQVSRR"
     gene            complement(3171..3353)
                     /locus_tag="VN039_01780"
     CDS             complement(3171..3353)
                     /locus_tag="VN039_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44745.1"
                     /translation="MAVSRGLIMLCDTCYDQVIAEKLPDAKNFVADFEALFDTICIGC
                     TDINRPLIDDMAGSGE"
BASE COUNT          709 a          914 c         1053 g          711 t
ORIGIN      
        1 ccatccgact cccacatata cgagcgcctc agtcctatcc tcctgaatga ggcgatgatg
       61 ttcactacag cccatgccct ttcggcactc atgatctcgc ggaccttgtg ctgggcggaa
      121 tagcctacgc cgttgcggat gagatgaccg aaagcaagga ggaaagggat gagttgcccg
      181 cgttgcggag gaatgtcgac agccgacctt gccgctgcga tgaaccaatt ggtctgtggg
      241 tgggcggtca ttgagggtcc ctattgtcat tgcgacgaga tcgaaagttc gatgcgaacg
      301 tgtccactgc gagagaagcg cgacaattct acagagcggc cggatggcag cgcgacatcg
      361 gagaggagaa gaccatgatc ggctatggga aacgtttgtt gctcatccat gatccggaga
      421 caacacaact gcctctcacc accatactgg agcaggaggg atttgtcgtt gtgcaggtgc
      481 atggtggggt tcaggcactg tgcgagatac agtttcgcca ctttgatgcg gtcgtcatcg
      541 gctctcacat ccctgacctc aatgggctcg atctcctgaa gcaatcccga acgacctggc
      601 ccgagatacc gatcatttta ttcgtggaag ccgactggga taagtgcgac gtagcggaag
      661 ccataggagc ttttgcttgg attcgaaagt catccgacct cagcatcctg ttgagtatgc
      721 tgagcttggc tatgaatcag agagaggaac gggaatccga tcaggcgcac cattataaaa
      781 taaagagcgg gctgcgccac agcggtaaca tgccggctcg gtgcgaaggg gtgtcggaat
      841 tcctgtgagg ggataactcg ggtgccgctt atcttcctct gttgcctcgt gatggccagc
      901 gcagtcctct catttgggct gatgctgtgg ttcgtcgtgc tgggaattgt gtggttcgtc
      961 atgccgggca tggaggaggg gtgagggaaa ccgacagtgt gtaccatcgt atgcttggcg
     1021 aaccgaggtc taggcatcgc tcctagagca gcttatatga gaaaagtgtc gaggctcaac
     1081 tcaaggggag attccagcgc tcatgcgatg agtgatggca gcggtctcgg tgaaacgtat
     1141 caaggattgg aggggcaccg atgtttcaac aggcagagca acagagagag catggagagc
     1201 aggaggcacg gatgccggcc gcagtgcgca ttctgctggc cgtggacgac tccgacaact
     1261 cccgtcgcgt ggtccagtat gtgggctcac tcctgagccg aacacccgac gccgtcatca
     1321 ccctctttca tgtgctgaag cccatgccgc ggcggttcct ggagcatggg ggatcggaga
     1381 atccggcggt cgaggcgcaa ttgagcatgc agttgcgcca tgaacaggac gcctggattc
     1441 agaaggaacg ggaatccgag tgtcatgtcc tcaagcaggc ctgtgagacg ttgacccgag
     1501 cggggttcga catgaaccgg gtgacggtca agtatgggca tgacgacgat attgccagaa
     1561 atatcctgga agaggcccgc aacgggcatt acgataccat cgccgtggga cggcagggga
     1621 tctcgcggat caaacagctg ttcgggggcg gcacgaccga ccatctcgtg catgccgcca
     1681 aaggagttgc gatttgggtg gtggcataag agggacaatg ccgctcacat tgagctggac
     1741 aagccggacc atgctcgcca ttgttgagga ttgatgtctg atgtctatgg ggaagccgcg
     1801 cgtggtgatt ctgggtggcg ggttcggtgg gatgtatgcc gcgctggaat ttgaacgggc
     1861 gctggcacgc ggagccgact tggacgtcac cctggtcaac cgcgacaact tttttctctt
     1921 caccccgatg ctgcacgaag tcgccgcgag cgatctcgat atcaccaata tcgtcagccc
     1981 gatccgcaag ctgttgcgtc gcgtgacgtt ctttcacggc gatatcgaag ccatcgatct
     2041 cgtgcacaaa cgagtcggcc tgtcgcatgg acacgagcag cattgtcatt cgctgccgta
     2101 cgaccatctg gtgctggctc ttggctcaac cacgaacttc ttcaacatcc ccggtttggc
     2161 agagcgggcg ctcaccatga aatcgctaag tgacgcgatt gctctgcgca accacctcat
     2221 cgcgaatctg gaggaagccg actttgagtg cggggcgtcg ctccgcgcac ccttactgaa
     2281 cttcgtcgtg gccggcggcg gcttcgccgg ggtcgaaacc atcgcggcca tgaatgactt
     2341 tctgcgggag gcggtgcggt tttttccgca tattcgggaa gacatgcttc ggatcatcct
     2401 ggtcagtgcg gggaaggtca tcttgccgga actgggggag gagctgggca cctacgcaca
     2461 gcgcaaactc agagagcaga aggtagaaat ccatgcgaat tgtaaagtca ccgccgtgac
     2521 ggaccgcgac gtcacgctca gcgatgggac gacggtgacg accaatacgc tggtctggac
     2581 ggccggaatt tccccacatt ccctcttgga taccctgccc tgcccgaaaa tgcgaggccg
     2641 tgtcctcgtc aacgaatatc tggaagttcc cgggtggccg ggcctctggg ccttcggcga
     2701 ctgcgcgctc gtgccgaatc gcaaaacggg ggaataccac cccccgaccg cgcaacacgc
     2761 gctgcgtgaa gggcgggtgg cggcgcggaa tatcctggcc accctgcgag gggagcgcat
     2821 gaagcccttc gtctactcga cgctcgggct cctggctccc atcgggaagc ggaccggggt
     2881 cgcgaccatt ctcggcgtga agttttcagg cttcatcgcc tggtggctgt ggcggacgat
     2941 ctacttgttg aaactgcctc gctttgagaa gaaagtcctc gttgccttgg actggaccct
     3001 tgatgtgctc ttttcgaagg atctggttca cttcagaacg actcgcccgt ctaccttacg
     3061 accgacggaa ggggagttgg aaagcacggc ggtccaggtt agtcggcgct gatctggtat
     3121 cagtgggtta cgcggtggtg actttaccaa tccgaaaaaa cgacaggaag ctactcgccg
     3181 ctcccggcca tgtcgtcgat gagggggcgg ttgatgtcgg tgcagccgat gcagatcgta
     3241 tcgaagagcg cttcaaaatc ggcgacaaaa ttctttgcgt ccggtagttt ctcggctatc
     3301 acctgatcat agcaggtgtc gcagagcata atgaggccgc gcgagaccgc cactgttttc
     3361 cttcccccgc tactcatgta ttacacc
//
LOCUS       DATMTI010000102         1815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_810180, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000102 DATMTI010000000
VERSION     DATMTI010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810180"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1500)
                     /locus_tag="VN039_01785"
     CDS             complement(322..1500)
                     /locus_tag="VN039_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV44746.1"
                     /translation="MMMNERQPMALIIDDDSVVRLLAREVLEQSGWMVDEAENGRIGL
                     EAFVRRRPDLVLLDIVMPERDGFAVCTELRRLLEGMHVPVLIMTGLEDYQSITQAYDA
                     GATDFIVKPINGLLLGHRVRYMLRASQAMRDLRESQGKLMQARDAALEGARLKSEFLA
                     TISHEIRTPMNGIIGMDELLLDTDLTPEQREYAETIKLSAEALLEIVNDMLDFSNLDA
                     GRATLSRERFSLSRVVDEQMALYRDRAREKGILLRHDIAWTVPAELWGDPARLGRLLS
                     VLLGNAVKFTERGEVHLFIEAAPRPIRAKPDAVSDVSQIRFSVIDTGIGIDTADAHRV
                     FQPFVQADGSYQRKYGGAGLGLALAKQLVELMGGTIDLESQPGKGSRFWFDLPLTQVG
                     "
     gene            1627..>1815
                     /locus_tag="VN039_01790"
     CDS             1627..>1815
                     /locus_tag="VN039_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44747.1"
                     /translation="MRTLVSFEAARRWPFRQHMLFGDLYEGLHQLLRVSVRIDVVHNW
                     FELPRPTACASHTMLAILS"
BASE COUNT          380 a          546 c          471 g          418 t
ORIGIN      
        1 aatgcgaaac ggctgaaccc gtttgaatgc tacctgacgg tctgggtggg gctgtgcatg
       61 gtggccggaa ttgtcctctg ccaatcggct cccgccgccg tgcgtcatgt cggcaagcgg
      121 ctacgcgctt tgctggtgac gctgtttgtg aaatcgcgca aaccgtaacg tcgcaccatc
      181 cccggccctt ccttcggcgc actatggaca gtggatcaca tcgtgatagc tctgtgaagc
      241 cgaaaaattg attgatgaca tgccctagtt cattattcgg cgaccaaggc acagccaggc
      301 tcacttggtt ccgggtgaga gttaccctac ctgtgtcagt ggtaggtcaa accagaagcg
      361 actgccttta cctggttggc tttcgagatc gatagttcca cccattagct cgaccaactg
      421 cttggcgagc gccaacccga gaccagcccc tccatacttg cgttgatatg acccgtctgc
      481 ttgaacgaac ggctgaaaca cccggtgggc atccgccgta tcgatcccta tccccgtatc
      541 aatcacggaa aatcggattt gactcacgtc ggacaccgca tcgggcttgg cccttatcgg
      601 ccgaggtgcg gcctcaatga aaagatggac ctccccgcgt tccgtaaatt tcacggcgtt
      661 gccgagtaag acggacagaa gacggccaag acgagccgga tcgccccaga gttccgccgg
      721 cacggtccat gctatgtcgt gacgcagcag gatacctttc tctctggctc gatctcggta
      781 cagcgccatc tgctcatcga caacccgact caacgagaac ctctctcggc tcagcgtggc
      841 ccgcccggca tccaggttgg aaaaatctaa catgtcgttc acgatttcga gcaaagcttc
      901 ggccgaaagc ttgatcgtct ctgcatactc ccgttgctcc ggtgtgagat ccgtatcgag
      961 tagcaattca tccatcccga tgataccgtt catcggcgtt cgaatctcgt ggctgatcgt
     1021 cgctagaaac tcagacttca agcgggcgcc ttcaagagcg gcgtcgcggg cctgcatcaa
     1081 tttaccctgg ctctctcgca ggtcgcgcat agcctggctg gctcggagca tgtaccgcac
     1141 tcgatgccca agcaagaggc cattgatcgg cttgacgatg aaatcggtgg ctccggcatc
     1201 ataggcttgg gtaattgact gatagtcttc cagtcccgtc ataatcagca ccgggacgtg
     1261 catgccttcc agcaatcgtc gcagctcggt gcagactgca aaaccatccc tttccggcat
     1321 cacgatgtcc agcaacacca aatcaggacg tcggcgaaca aatgcctcga gtccaatgcg
     1381 cccattttca gcctcatcga ccatccaccc cgattgttcg agcacttcac gggccagcaa
     1441 tcgcacgacg ctatcatcgt cgatgatcaa ggccatcggc tgccgttcgt tcatcatcat
     1501 gccgccattc tccctttggt gagtgcttgc tgaaaggcgg cacacgccgc tgcgtagtgc
     1561 cgttcgagtt tactcaatat ttcatcggct cggccaacct aataacgcgg atggtgggtc
     1621 taccgtatgc gtacgttagt gtcattcgaa gcagcaaggc gctggccgtt tcgtcagcac
     1681 atgttgttcg gcgatctcta cgaggggctg catcaattac tgcgggtctc tgttcgaata
     1741 gatgtcgtcc ataattggtt tgagctccct agaccgactg cgtgtgcgtc gcacacaatg
     1801 ttagctatcc tatca
//
LOCUS       DATMTI010000103         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_725221, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000103 DATMTI010000000
VERSION     DATMTI010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725221"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VN039_01795"
     CDS             complement(<1..267)
                     /locus_tag="VN039_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWV44748.1"
                     /translation="MNLTLHPMKEIRVIIAGEHRAFVTDLLDRVKATGYTIIGNISGK
                     GHHGVREAHFMFSEQESLEIIMTIVPEEKVEPILAGLRPLFERHS"
     gene            complement(319..>2442)
                     /locus_tag="VN039_01800"
     CDS             complement(319..>2442)
                     /locus_tag="VN039_01800"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HWV44749.1"
                     /translation="PALYAEEVDRLSHRKGQSWNNILTRYRAEVIPRLQAAVRRGAAR
                     RLLALAHSLRIDATVLVESAPVDLKQVVDWMEAFPESDHGPVWLKALEAGYQEQLLGR
                     LRARTEHVPSSETPRPYSQSVYCIDVRSEPFRRHLESLGPHETYGFAGFFAAFIRYRA
                     WGKEHDTEQLPVIMRAKNEVREIPRSYLDHKVSLHAARAKWVHAGHTLLHDLKENVVT
                     PYVMVESLGWFYGLPIFGKTLIPSLYQRWTAWLRRLFVPSLATTLTIDKLAPRETAEM
                     LEAEQQAVIREALYEQLGLRSFRISPALIEGLRQRALAGEEDPEPPIAAAEVERAGLT
                     PETINAFVETLRRQYDLTARSASRHRERLTRTGFTLEEQVLTVDTALRMMGLTKNFAR
                     LVLFCAHGSTSDNNPYESALDCGACGGNEGKPNARALAMMANNPKVRERIAKLRIEIP
                     SDTHFLAGQMDTTTDAITLFDLEDAPPTHRADIARFQEDLRDASVLTSQERCTRFPDI
                     QQVIPETAAEAHVRRRSIDWSQVRPEWGLSNNTSFLIGRRELTKGLDLGGRVFLHSYD
                     YREDPSNRLLEVLLTAPQVVAQWINMEHYFSAVDNEVYGSGSKIYHNVVGRLGIMSGP
                     WSDLRLGLARQTVMSGEGAYHEPMRLLTIIETPRARIEKLIARHDVLQHFYHNEWVHL
                     VALDPEDREWYRYRPDGTWHPIVRL"
BASE COUNT          484 a          702 c          718 g          538 t
ORIGIN      
        1 ggaatgtcgc tcaaagagcg gacggagtcc ggcaagaatg ggttcgactt tttcttccgg
       61 cacgatcgtc atgataattt caaggctctc ctgttcgctg aacatgaaat gcgcttcgcg
      121 taccccatgg tgtcctttcc ctgagatatt gccgataatc gtgtagccgg tcgctttcac
      181 tcgatccaac agatcggtca cgaacgcccg gtgttccccc gcgatgatca cgcgaatttc
      241 cttcatcgga tgcaaagtca agttcatcag gtgtcctcct ccggcatgtt ttaatcttct
      301 ctcgaatgga tgccgtttct aaagtcttac gatcgggtgc caggtcccgt cgggacgata
      361 gcgataccac tcccggtctt cggggtcgag cgcaaccaga tggacccatt cattgtgata
      421 aaaatgctgt aagacatcgt gccgggcaat caatttctcg attcgcgcgc ggggcgtttc
      481 aatgatcgtc agcagccgca tcggttcatg gtaggcgcct tcgccactca tgacggtttg
      541 cctggccaat ccaagtcgga ggtcgctcca gggtccggac atgatgccga gccggccgac
      601 cacattatga tagatcttgc ttccactgcc atagacctcg ttatcgacgg cggagaaata
      661 atgttccatg ttgatccact gcgccacgac ctgcggggcc gtgagcagca cttcgagcag
      721 tcgattactc ggatcttctc gataatcgta tgagtgcagg aagactcgtc cccccaggtc
      781 caagcctttc gtcagttccc gccgtccgat caggaacgat gtattgtttg agagacccca
      841 ttccggtctg acttgactcc agtctatgct tcgcctacgg acatgcgcct cggctgccgt
      901 ctccggaatg acttgctgga tatcgggaaa gcgcgtacag cgttcttgac tggtcagtac
      961 ggaagcatcc ctgagatcct cttggaatcg ggctatgtcc gcgcgatggg tcggcggggc
     1021 atcttcgagg tcgaaaagcg taatggcatc ggtcgtcgta tccatttggc cggctagaaa
     1081 atgcgtgtcg gaagggatct cgattctcag tttggcgatt cgctcgcgga ccttgggatt
     1141 gttcgccatc atcgcgagag cacgggcgtt gggcttgccc tcattgccgc cgcaggctcc
     1201 gcaatcgagc gcggattcgt aggggttatt atccgacgtg ctcccgtggg cgcagaaaag
     1261 aacgagccgc gcaaagttct tggtcagccc catcatgcgg agtgcggtgt cgaccgtcaa
     1321 aacctgctct tccaaggtga aaccggttct tgtgagtcgt tccctgtgtc gtgacgcgga
     1381 tcgcgccgtg agatcgtact gccgccgcag agtctcgaca aacgcgttga tcgtctccgg
     1441 cgtaagcccg gcccgctcga cttctgcggc ggcaatcggc ggttccggat cttcttcccc
     1501 tgccagcgca cgctgccgca agccttcgat gagcgcgggc gaaatacgga agctgcggag
     1561 cccaagttgt tcgtacaagg cttccctgat cacggcctgt tgttccgctt cgagcatctc
     1621 ggcagtttca cgcggtgcca gcttatcgat cgtcaacgta gtggccagag aggggacgaa
     1681 cagacgtcgt aaccaagcgg tccagcgctg atagagggag gggatcaatg tcttgccgaa
     1741 aatgggtagg ccgtaaaacc agccaagcga ttccaccatg acatagggag ttacgacgtt
     1801 ttccttcaga tcgtgtagga gcgtatggcc ggcatgcacc cacttggccc gcgccgcgtg
     1861 cagtgagacc ttatgatcga gatagctgcg cggaatctcc cgcacctcgt tcttggcccg
     1921 catgatcacc ggcaactgtt ccgtatcgtg ttccttgccc caggcgcgat agcggatgaa
     1981 agcggcaaag aaaccggcga agccataggt ctcgtgcggg ccgagcgatt cgagatggcg
     2041 gcggaacggt tcggagcgca cgtcgatgca gtagaccgac tgtgaatagg ggcgcggcgt
     2101 ctcagaggaa gggacatgct cggtccgcgc gcgtaagcgt cccaacagct gttcctgata
     2161 gccggcctcc agggctttga gccacaccgg cccatggtcc gattcgggaa aggcttccat
     2221 ccagtcgacc acttgcttga ggtccacggg ggcgctttca acgagtacgg ttgcatcaat
     2281 cctcaacgaa tgagccaggg cgagaagccg ccgcgcggct ccacgacgaa cggccgcttg
     2341 cagccgggga atgacttcgg cacggtaccg ggtgagaata ttgttccagc tttgcccctt
     2401 ccgatgcgaa agccggtcga cttcctcggc atagagggcg gg
//
LOCUS       DATMTI010000104         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_4106, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000104 DATMTI010000000
VERSION     DATMTI010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4106"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VN039_01805"
     CDS             complement(<1..302)
                     /locus_tag="VN039_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HWV44750.1"
                     /translation="MATLPAAEIRRAVRQGLEEDLTQGDATTAAIFSSPVPAHAEIIA
                     QQPLVVAGMAAAVQTFLLVDPSLQLSVSKRDGDRATNGERLLHIEGDGRSILQAERV"
     gene            complement(306..>2323)
                     /locus_tag="VN039_01810"
     CDS             complement(306..>2323)
                     /locus_tag="VN039_01810"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWV44751.1"
                     /translation="VRLPLTTREIPIVGDSILVDLEFGTGAVKITPAHDFNDYEAGER
                     HSLPRFSILDYEALLDLKGMKAALVEESVIKEVIQLPVTKARPKIEQLLRDRGFLVKT
                     EDHKMAIGKCYRCKTVVEPYLSPQWFVKIQLLADPAIKAVEDRCIRIVPENWINNYLG
                     WMRDIKDWCISRQIWWGHQIPAWYCRSCNTEILTVTGSSGRQYEHLYISPNAIPIVSR
                     TKPEKCDKCPSTDLIRDPDVLDTWFSSALWPFSTLGWPEQTQELKTYYPTSVLVTGLD
                     ILFFWVARMIMMGLKFMGDVPFKDVYIHALVRDAEGQKMSKSKGNVIDPLHVMDQFGT
                     DALRFTLASMASPGRDIKLAEERIEGYRNFANKIWNAARFALIYLDGPRTVRPSEQRT
                     FPDRWILSCLNQTIRAVTAELESYRFDRAATVLYQFIWHEYCDWYLELIKPVLQTPEH
                     PDGASTRHTLLETLEMTMRLLHPFMPFITEEIWQTIPHQGNSIVVQSYPVPDQAWTAP
                     DAEQHFALLEQTVGLVRTGRVLLNYPPGQQIPFHVGHNDPNRQNQLHQLQRHLAHLSR
                     GTAEVSPSHNWPAARLLRLITEGLSVGIVVAEDVDLKKAIDRLAKQITETDKELQRVS
                     GKLNNAEFVSKAPPDVIAEHQERVRTLSRDRALLADSEQQLRAMLAT"
BASE COUNT          479 a          622 c          664 g          558 t
ORIGIN      
        1 acccgctcgg cttgcaaaat ggatcgccca tcaccttcga tatggaggag ccgctctcca
       61 ttcgtcgctc ggtcgccgtc gcgcttggag accgacaact gcagggacgg atcgaccaag
      121 agaaaggtct gaaccgctgc ggccattccc gccacgacca acggctgttg cgcgataatc
      181 tcggcgtgag ccggtacggg agatgagaag atcgcagcgg tcgtggcgtc tccttgagtc
      241 aggtcttctt cgagcccttg gcgcactgcg cgacgaattt ctgcagcggg gagtgtggcc
      301 atcggtcagg ttgcgagcat ggcgcggagc tgctgttcgc tgtcggccag caacgcacga
      361 tcgcgggaca gagtccgcac ccgctcctga tgctcagcga tcacgtcggg cggagctttc
      421 gacacaaact cggcgttgtt cagtttcccg ctgacccgtt gcaattcctt gtcggtctcc
      481 gtgatttgtt ttgccaggcg atcaatcgct tttttgaggt cgacgtcttc ggcgactacg
      541 atgcccacgg aaaggccttc cgtgatgagt cgcagcaatc tggctgccgg ccaattatgc
      601 gacgggctta cttcggcggt gccgcggctg aggtgggcca ggtgtcgttg taactgatgg
      661 agctggttct gcctgttcgg gtcgttgtgt ccgacatgaa acggaatctg ctgccccggg
      721 ggataattca aaaggactcg accggttcgg acgagtccta ccgtctgttc cagcaacgcg
      781 aagtgctgtt ctgcatcagg cgcagtccat gcctggtccg ggaccggata gctctgcacg
      841 acgatgctgt ttccctgatg ggggatcgtt tgccaaattt cttccgtgat gaacggcatg
      901 aaggggtgca gcagccgcat ggtcatttcc agcgtttcca agagtgtgtg ccttgtgctt
      961 gctccatccg ggtgttcagg tgtttgcaga accggtttga tcaattccag gtaccagtcg
     1021 cagtactcat gccaaatgaa ttgataaagg actgtcgccg cacgatcgaa tcggtatgat
     1081 tccaactctg ccgtgacggc gcggattgtc tggttgagac agctcaagat ccaccggtcg
     1141 gggaacgttc gttgctccga tgggcggacg gtgcgtggcc cgtcgagata tatcaatgcg
     1201 aatcgcgcgg cgttccaaat cttgttggca aagttgcggt agccttcaat tcgttcttcc
     1261 gccagcttga tatcgcgtcc cggcgaggcc atcgaggcaa gggtgaaccg aagggcgtcg
     1321 gtgccgaatt gatccatgac gtgcagcgga tcaatcacgt tgcccttgga cttgctcatc
     1381 ttctggcctt ccgcgtcgcg gaccaaagca tggatataga cgtccttgaa gggcacgtct
     1441 cccatgaact tgagccccat catgatcatg cgggctaccc agaagaacag aatgtcgagg
     1501 ccggtgacaa gcaccgaggt cgggtagtag gttttcagtt cctgcgtttg ctctggccat
     1561 cccaatgtcg agaacggcca cagggcggag gagaaccaag tatcgagcac gtcaggatca
     1621 cgaataagat ccgtgcttgg acacttgtcg cacttttccg gttttgtcct actgacaata
     1681 ggaatagcgt tgggtgatat gtagagatgt tcatattgcc ggccagacga tcccgtcact
     1741 gttaatattt ccgtattgca agaccggcag taccacgctg gtatctggtg cccccaccag
     1801 atttgccgtg agatgcacca atctttgata tcccgcatcc agccgagata gttattgatc
     1861 cagttttcgg gaacaattcg tatgcatcga tcctccaccg ctttgatcgc ggggtcggcc
     1921 agaagctgaa tctttacgaa ccactgtggt gagagatagg gctcgacgac agttttacaa
     1981 cggtagcact tcccaatcgc cattttgtgg tcttcagttt tgacgagaaa tcctcggtct
     2041 cgtaaaagct gctctatttt ggggcgcgct ttagtaacag gtaactgaat gacttctttg
     2101 attacagact cttcaaccaa agccgccttc attcctttga gatcgagaag tgcttcatag
     2161 tctaaaatag aaaaccgagg caagctatgc cgctctcccg cttcatagtc gttgaagtcg
     2221 tgagccggag tgatcttaac ggcgccagtc ccgaattcca gatcgacgag gatcgaatcg
     2281 cctacgatcg gaatctcacg agtagtcagc ggcaggcgga ctt
//
LOCUS       DATMTI010000105         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_4218, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000105 DATMTI010000000
VERSION     DATMTI010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4218"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VN039_01815"
     CDS             complement(<1..348)
                     /locus_tag="VN039_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44752.1"
                     /translation="MEPNQDNLTADTLPDASQLNAPDGQAADADTLSLAELNTFLGKD
                     FKDKDAALKAVKDTFAYVGRKIETASPAPEATTGTSTLEADVRSLKEDLFYSQHPEYA
                     EYRSLIASMGNPTE"
     gene            complement(423..731)
                     /locus_tag="VN039_01820"
     CDS             complement(423..731)
                     /locus_tag="VN039_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44753.1"
                     /translation="MNKHIDTETQAILTEGEHYKALVDSEGWKLAHASLQDRIIDLQM
                     IGNVTGQTAEEKVRDMEARMMASNILFQWLQDVHGRVQQFEANKNLAPQDPIISRDDS
                     "
     gene            complement(728..>2290)
                     /locus_tag="VN039_01825"
     CDS             complement(728..>2290)
                     /locus_tag="VN039_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44754.1"
                     /translation="DLDQKDINFRSTNPDGYVITDLVRAYVRNKLSDMNFGQQLDDFE
                     RRLAIDGTAVWKVYEEGGKLINLPVDLLNIYIDPTTPSIQEAYRFTERSLMFPEQIKS
                     MSGWMNTDGIDKNVPTGLPRTDPLWVRQNAGMSNVKMVDVYETWGKIPKSLITGIASD
                     DGIEIEGHLVVSGIDSPGTERLHLIEENLDGAKPYEEAWYTRVPNRWYGRGVAEKLLM
                     LQTYSNLILNVRINRARVSQLGLFKIKKGAGITPQMLARLPSNGAVVLNDMEDLQQFV
                     MQEVGQSSYSDEGVVNSLSERLTNAFESVTGESLPSSTPATNAVLQNRSAMTGFTIIK
                     DELGMFLKRWMDRHALRILLKEMKGNDLIRFSTDDDQAQQLIDRIVVIKAHEALAAHY
                     EQGVIPSPIQIASAMEEAQSHLKQRGDLFIEMDSEVTADELECEVYITNEEMDVGVIL
                     QNLITMLQAAPEYHDTIVKEAFDLMGLAQPPKSQHGMPGAPQGAPANIPMPALPGAQL
                     QQATTAGNVPQL"
BASE COUNT          406 a          617 c          690 g          577 t
ORIGIN      
        1 ctcggtcgga ttacccatgc tcgcgataag cgagcggtat tccgcgtatt cgggatgctg
       61 cgagtagaaa aggtcttcct tgagtgagcg cacgtcggct tcaagggtag aagtgccagt
      121 ggtggcttcc ggcgctggag aagccgtctc gatcttgcgt ccgacatagg cgaacgtgtc
      181 tttgacggcc ttgagggcgg cgtccttgtc cttgaagtct ttgccgagaa acgtgttcag
      241 ctcagcaagc gaaagggtgt cggcatccgc cgcttggccg tctggtgcat tcaattgaga
      301 tgcatcaggt aaggtatctg cggtaaggtt gtcttgattt ggttccataa acagaatcag
      361 ttgagattag tagtgtcgga ctatcgacct tcattcataa gcccgccttc ggtatggccg
      421 gattacgaat cgtccctact gatgatgggg tcttgcggcg cgaggttctt gttcgcctcg
      481 aactgctgta cgcgcccgtg cacgtcctgg agccactgga acaggatgtt tgatgccatc
      541 atccgtgctt ccatatcgcg caccttttcc tcggcagtct gcccggtaac attgccgatc
      601 atctggaggt cgatgatgcg gtcctggagg gatgcatggg cgagcttcca tccttctgaa
      661 tcgacgagcg ccttgtagtg ctcgccctcg gtgaggatag cctgcgtctc cgtatcaatg
      721 tgcttgttca taactggggc acgttaccgg ccgtggtcgc ctgctggagt tgcgctcctg
      781 gcaatgcggg catcgggatg ttcgctggtg ctccctgcgg cgcgcctggc attccatgct
      841 gggatttcgg cggctgcgcc aggcccatga ggtcgaatgc ttccttgacg atggtatcgt
      901 ggtattcggg agcggcctgg agcatcgtaa tgaggttctg aaggatgacg cctacgtcca
      961 tctcctcgtt cgtgatgtac acctcgcatt ccagttcgtc ggcagtgact tcgctgtcca
     1021 tctcgatgaa tagatcgccg cgctgcttca ggtgcgactg cgcctcctcc attgcagagg
     1081 ctatctggat tggcgatggg atgacgccct gctcataatg agccgcgaga gcctcgtggg
     1141 ccttgatgac gacgatgcgg tcgatgagct gctgcgcctg gtcgtcgtcg gtcgagaagc
     1201 ggatgaggtc gttgcctttc atttccttga ggaggatgcg gagcgcatgc cggtccatcc
     1261 accgcttcag gaacatgccc agctcgtctt tgattatggt gaagccggtc atggcgctgc
     1321 ggttctgaag caccgcattc gtcgctggcg tcgaagatgg caggctctcg cccgtcactg
     1381 actcaaatgc gttcgtgagc cgttcggaga ggctgttgac gacaccttcg tcgctgtagc
     1441 tcgactgccc gacttcctgc atgacgaatt gctggaggtc ttccatgtcg ttgagcacta
     1501 ctgcgccatt cgaggggagc cgtgcaagca tctgcggcgt gatgcctgcg ccctttttga
     1561 tcttgaacag cccaagctgc gagacgcgcg ctcggttgat gcggacgttc aggatgagat
     1621 tggaataggt ctgaagcatt aggagcttct cggccactcc tcggccgtac cagcggtttg
     1681 ggacgcgcgt ataccacgct tcttcatagg gcttcgcgcc gtccaagttc tcctcgatga
     1741 gatgcagccg ttcagtaccg ggactgtcga tgccggagac gacaaggtgg ccttctattt
     1801 cgatgccatc atccgatgcg atgccggtta tcagcgactt cggaatcttc ccccatgtct
     1861 cgtatacgtc caccatcttc acattggaca tgccggcgtt ctgccgtacc cagagcggat
     1921 cggtacgcgg caatcctgtc gggacattct tgtcgatgcc gtctgtgttc atccaacccg
     1981 acatgctctt tatctgttca gggaacatga gggagcgttc tgtgaaacga tacgcttcct
     2041 ggatggaagg cgtcgtcggg tcgatataga tattcaaaag gtctacgggg agattgatga
     2101 gcttgccgcc ttcttcgtac actttccata ctgcggtgcc atcaatcgcc aggcggcgct
     2161 cgaaatcatc aagctgctgg ccgaagttca tatcggagag tttgttgcgg acgtaggcgc
     2221 gtaccaaatc cgtgatgacg tagccgtcgg ggttcgtcga gcggaagttg atgtccttct
     2281 ggtcgaggtc
//
LOCUS       DATMTI010000106         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_343744, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000106 DATMTI010000000
VERSION     DATMTI010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343744"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VN039_01830"
     CDS             complement(<1..563)
                     /locus_tag="VN039_01830"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWV44755.1"
                     /translation="MASAFSHAVVAVALGKAFQHPIMTWPVLLSGAVCSIVPDLDVIG
                     FYFGIHYGDLWGHRGMTHSLLFAGLLSTVLVGMWSKQKTRAAQVGIFVYLFFCTASHG
                     VLDALTDGGLGVAFFSPFDTSRYFFAARPVAVSPIGISTFFSEDAVHILATEVTWIWL
                     PTIAGFFIVRGLQYAQSAQSASKQVPRG"
     gene            complement(595..1056)
                     /locus_tag="VN039_01835"
     CDS             complement(595..1056)
                     /locus_tag="VN039_01835"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HWV44756.1"
                     /translation="MFDVILYQPEIPPNTGNIIRLCANTGVRLHLVKPLGFSLEDKQL
                     TRAGLDYREFATISAYDNWAACEERFADSRLFAVSTKGTRRYDQIAYAKNDVFLFGPE
                     SRGLPTELLEAVSEQRRIRLPMTPGSRSLNLSNTVAVVVYEAWRQIGFDID"
     gene            complement(1157..>2406)
                     /locus_tag="VN039_01840"
     CDS             complement(1157..>2406)
                     /locus_tag="VN039_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44757.1"
                     /translation="CTSMAYLPRTSLARLSQGREELQVNELAHSFTSMFRALNDRVAP
                     LGCGLTGGRDTRVVVAAVIASGVPASFVTMGSTESADVRIATKIAETFHLTHRVGYPQ
                     GVVTDRWEEGVRRLVRQTDGMVNLWQVANSISQIQQIESLPLSVWGVGGEIASGAYYR
                     ILRLPPTLDDSQMLKTFLSSLVSKDHELLKQETRQLALQNVRETCQAFLDDGFEPQAV
                     PDAFYTFDRVRRWAGTNTRKASPVCDMFTPFCTIPYIEAALAMPLPDRITGRLHRELI
                     RAMTPSLLDLPYDKQWHPVPPKLGVKRLVAQTVREAAPRWFLRVLRYGASQLQQRKSE
                     GKTGPVQAEWVEAKRLAIQDVCLNQRSSVLWDYVDRRSFEDIMSSKTAGKVRRQHCAK
                     ILSIATLFYYEAEQLDRSGLEKR"
BASE COUNT          585 a          627 c          652 g          542 t
ORIGIN      
        1 cctctcggta cttgcttcga agccgattga gccgattgtg catattgtag tccgcgaacg
       61 atgaagaacc ccgcaatggt tgggagccag atccatgtca cctcagtggc aaggatgtga
      121 acggcatcct cgctaaaaaa tgtgctgatg ccgatcgggg acacggccac cggccgagcc
      181 gcgaaaaaat atcggcttgt atcgaacggg gagaagaagg ccacaccgag tccgccgtcg
      241 gtcagcgcat cgagcacacc atgtgatgcg gtgcagaaaa agagataaac gaatattccc
      301 acctgcgctg cccttgtttt ctgcttggac cacatgccca cgagaacagt gctcaatagg
      361 ccggcaaaca agagagaatg ggtcatgccg cgatgacccc agagatcgcc gtaatggatg
      421 ccgaagtaga agccgatcac atccagatcc ggcacaatag agcagactgc acctgacaat
      481 aagacaggcc atgtcatgat gggatgttga aacgctttgc ctagtgcaac ggcgacaacg
      541 gcatgggaga acgcagaggc catacttggt tttcgtaagg ctgcaggcag gctctcagtc
      601 gatgtcgaat ccgatttgcc tccaagcctc gtacaccacg actgcgacag tattggaaag
      661 attcaaactt ctgctcccgg gcgtcatagg aaggcgaatc cgtcgctgtt cggacactgc
      721 ctccaacaat tcggtcggca gtcctcggct ttccggtccg aacagaaata catcgttctt
      781 tgcgtatgca atctgatcgt aacggcgagt gcctttcgta gagacggcaa atagacgact
      841 gtctgcaaaa cgttcttcac aagccgccca gttatcatag gcgctgatgg ttgcaaattc
      901 gcggtaatcc aatccggcgc gtgtcagctg cttatcttcc agcgagaacc ccaacggttt
      961 gacaaggtgt aggcgcacac cggtgttggc gcagaggcgg atgatattgc cggtattcgg
     1021 gggaatttcc ggctgataaa ggatgacatc gaacatagag aggaaaagtg tatcatgtgg
     1081 aacgtgctca aatgagcaca ataggcgcaa gcctgatttg ggtcgggttt gaatggtcct
     1141 caaacctaat agagttctat cttttctcca gtcccgaccg gtctagctgt tctgcctcgt
     1201 agtaaaacag tgtagcaatg gacaaaatct ttgcgcagtg ttgccggcga acttttcccg
     1261 cagttttgga cgacatgata tcttcaaatg atcgacggtc gacgtagtcc cacaatacgg
     1321 aactacgttg gttgagacag acatcctgga tcgccaagcg ttttgcttcc acccactcag
     1381 cctgcacagg accggttttc ccttctgact tcctctgttg taactggcta gcaccgtacc
     1441 gcaaaacccg caggaaccat ctcggagccg cttctctaac agtctgggcc accaaccgct
     1501 taacacctag ttttggcggg actggatgcc actgtttgtc gtagggtagg tcgagtagtg
     1561 atggtgtcat agcgcgaatg agctcgcggt gtaaccgtcc ggtgatcctg tcaggaagtg
     1621 gcattgcaag agcggcctca atatacggaa tggtgcaaaa aggagtgaac atgtcgcaaa
     1681 ctggagatgc ctttcgtgta ttggtgcccg cccagcgccg aacgcgatca aatgtgtaga
     1741 atgcgtccgg cacggcctgc ggctcaaatc cgtcatccag aaaggcttgg catgtttcgc
     1801 gcacgttttg gagagcgagt tgtcgagttt cctgtttaag taactcatgg tctttcgaca
     1861 ccaggctaga aaggaatgtt ttaagcattt ggctgtcgtc aagtgtcggt ggcaaacgca
     1921 ggatacggta ataggctccg ctagcgattt cacctcctac tccccagact gacaaaggta
     1981 gactttcaat ctgctggatc tgcgagatgg aatttgcaac ctgccacaga ttgaccatgc
     2041 cgtcggtctg tcgaaccaat cggcgaaccc cttcttccca acgatccgta accactccct
     2101 gtgggtagcc gacgcgatgg gtcaggtgga aggtttcggc aatttttgtt gcaatccgaa
     2161 catcggcact ttccgttgag cccattgtga caaaagaggc tggaacgccg gacgcgatca
     2221 ccgcagccac aacgacccgg gtatctcgac cgccggttag gccgcaccct aatggggcaa
     2281 cacggtcatt cagcgcgcga aacatagatg taaagctgtg tgccaattca ttaacctgta
     2341 gttcttcgcg gccttgactt aaccgtgcca ggcttgttcg gggtaggtaa gccatggagg
     2401 tacagg
//
LOCUS       DATMTI010000107         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_46602, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000107 DATMTI010000000
VERSION     DATMTI010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46602"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(535..1089)
                     /gene="tadA"
                     /locus_tag="VN039_01845"
     CDS             complement(535..1089)
                     /gene="tadA"
                     /locus_tag="VN039_01845"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HWV44758.1"
                     /translation="MTIKGSSTSLSMQQEPHDSHYMELALQQAVLAPHAGEVPIGAVL
                     VHNHEVIAAAHNYREISQDPTAHAEMIVIRKAAERLQTWRLNETTLYVTLEPCPMCAG
                     AIVQSRIARLVFGAWDPKAGACGSIFDIPDERRLNHRVQVTGGLLERESRELLQEFFR
                     AKRGTLSESSSSPKPSSYTPKRNQ"
     gene            complement(1197..1850)
                     /locus_tag="VN039_01850"
     CDS             complement(1197..1850)
                     /locus_tag="VN039_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44759.1"
                     /translation="MTHDELAAAVPLYTVGALERIERQALEVHLLSGCIPCHTTLKEY
                     QSVAVTLPFGLNIAPPPRSLKGKIMSARVSPPGTELETQSPSKPSLEPGEWMKHLFPP
                     SPPPGSSFGWALGMVIFVVLIILVFFGWNASTQVTEDMEKLAQLRSQAEEMGSKLGTL
                     QQQLSDRAELLAQTREELQRRTAELVEIKDQLIQREAELEELKVLLAQGGGRSARVP"
     gene            complement(1847..2452)
                     /locus_tag="VN039_01855"
     CDS             complement(1847..2452)
                     /locus_tag="VN039_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV44760.1"
                     /translation="MDTSFQLAASIIDPKLVARVAKGEHHALSQLYDQSSTVLFSLAL
                     RILGNREEAADVLQEIYLDVWRKIVRYDVGRGTPIAWLIMLTRSRAIDRLRTRGPRTF
                     RQMSSSIDESLTSQVPGESSGLFDSQADQELQYLIKEAWANLPQIQQQVVEMAYYDGL
                     PDAEIAAQLNQPVETVKTCIALGMSQLRESLQSYCIQDEAV"
     gene            complement(2579..>2762)
                     /locus_tag="VN039_01860"
     CDS             complement(2579..>2762)
                     /locus_tag="VN039_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44761.1"
                     /translation="LVVRADPLYVGTCTLNPWHLYMRPLLQSITTKVLSESIGLFLPT
                     KTPRHSAPPSTAFGKQ"
BASE COUNT          647 a          717 c          797 g          601 t
ORIGIN      
        1 catggaacgt cattgcggtc atacaccgtc ctacagaatc aaatcgtgtg ttgagacgtc
       61 accctaatca tcgtggacga caccacaatc agcatttggc gggcgtccta gggcgtaccc
      121 tgaaagacga gccatcgcaa aggggaatca tcgccgattg acacatggga taacaccatt
      181 cctgccacca ggaaaaacac aacagacgtg gtcggcatga cccacagggc gctacgaagg
      241 cgatcccaca acactcgacg ccgagtaagc acgttcatat tgcgatttcc ggtttgagta
      301 cgcctctatg caatgaagat ctatacttgc gaggctgggg gagaaggcgt actggattga
      361 ccatgacgcc accgctccaa gggcgctcac gccgggagat ggagggtaca atcactgaac
      421 cagcggtacg gtcggagcca gtatagcgag attgctctcc actaagcaaa acccatcatg
      481 accccacaaa aggtaaggaa aactgtggat gggagaaaga gtggacaggc agcgttactg
      541 gttgcgtttc ggtgtataag aggacggctt cggagaactg ctgctctccg acagagtacc
      601 tctcttggct cgaaagaact cctgaaggag ttcacggctc tcccgttcga gcaggcctcc
      661 cgtcacctgt actcgatgat tgagccggcg ttcgtctgga atatcaaaga tcgatccgca
      721 agcaccggct ttgggatccc acgctccgaa tacaagccgc gcaatgcggg attgcacgat
      781 cgctccggca cacatggggc aaggctccag cgtgacataa agcgtcgtct cgttgagccg
      841 ccatgtctgc agccgttcgg cagctttccg gatcacgatc atttccgcat gcgctgtagg
      901 gtcctgcgaa atctccctgt agttgtgagc ggccgcgatt acttcgtggt tgtgcacgag
      961 gactgcgccg atcgggactt ctcctgcgtg aggggcaaga acagcctgtt ggagagccag
     1021 ctccatgtag tgggaatcat gcggttcttg ctgcatcgaa agagatgtcg aggagccctt
     1081 gatggtcaca gtgcatgcta gcataggagc ttgagagagg gacaggacag agaagcggga
     1141 tgaggcggac tggcttccgg cgacggggca aaaggaagat gggcagatgg ttcgacctag
     1201 ggaactcgcg ctgatcgccc gcctccctga gccaataaaa ctttcagctc ttccagctcg
     1261 gcttctcgct gaatcaactg atcttttatc tccaccagtt cagccgtgcg tcgttgcagc
     1321 tcttctcgag tttgggcgag tagttccgct cggtcgctga gctgctgttg gagagttccc
     1381 agtttagagc ccatttcctc cgcttgagac cgcagctgag ccagcttctc catgtcttcg
     1441 gtcacttggg tggaggcgtt ccacccaaaa aataccagga tgatcaggac cacgaagatg
     1501 accattccca acgcccaccc gaatgacgag ccgggtggag gtgacggtgg aaagagatgt
     1561 ttcatccatt cacccggctc aagactcggt ttggaaggag attgggtttc taactcagtg
     1621 ccggggggcg acacacgagc cgacataatc ttgcctttca aggatctggg aggaggcgcg
     1681 atgttgaggc caaacggtaa tgtcaccgct accgactgat actccttcag cgtcgtgtgg
     1741 cagggaatgc aacctgaaag cagatggacc tccagggcct gtcgctctat ccgttcgagc
     1801 gctccgacgg tgtataaagg aacggcggca gctaattcgt catgtgtcat accgcttcat
     1861 cctgtatgca atatgattgc aacgattcgc gcagttgaga catgccgagc gcgatgcagg
     1921 tcttcactgt ctccaccggc tgattaagct gcgccgcgat ctccgcatcg ggtaagccgt
     1981 catagtaggc catctccacc acttgctgtt gtatctgcgg caagttcgcc caagcctctt
     2041 tgatcaggta ttgcaactct tggtcggcct gtgaatcaaa aagaccggaa ctttcgccag
     2101 gaacttggct agtcagggat tcatcgatgg acgagctcat ctggcggaag gtgcgagggc
     2161 cacgtgttcg caatcggtcg atagcccggc tgcgggtcag cataatgagc caggcgatag
     2221 gagtgcctcg gccgacatcg tagcgaacga tctttctcca aacgtcgagg tagatctcct
     2281 ggagtacgtc cgctgcttct tcgcggttgc cgaggatgcg taatgccaga ctgaataata
     2341 cggtactgga ttgatcgtag agctggctca gagcatgatg ctcgcctttg gcaactcggg
     2401 ccactagttt cggatcaatg atcgaggcgg cgagctgaaa agatgtatcc ataagcatgt
     2461 tgattgtggt gatcgaggtg atgtccgttg accggtcatc actatagcat cctcgatcca
     2521 ctcgacgaag aaatcgcaca aaacgatgcc gagtgtttca cgacagaaaa cgaaaatact
     2581 attgctttcc gaatgctgtt gagggcgggg cggaatgacg aggagtttta gtcggtagga
     2641 ataagccgat cgattcagaa aggactttcg tggttatgct ttggagcagc ggtcgcatgt
     2701 atagatgcca aggattgagc gtacaggttc ccacatagag agggtctgca cggacgacaa
     2761 gc
//
LOCUS       DATMTI010000108         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_4447, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000108 DATMTI010000000
VERSION     DATMTI010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4447"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..626)
                     /locus_tag="VN039_01865"
     CDS             complement(180..626)
                     /locus_tag="VN039_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV44762.1"
                     /translation="MIGYGKRVLVIGDEEFVDSFLIGELDLHHFVVVAVADGLRVLSE
                     LRRRHFDAVIADMQTPNLNGLALLYQCQLVWPQLPIILMSDHYEDVVDLAINRGVYAY
                     LPKPVEHGKLIRVLSEAMTHLVDPGPQREQLSEVFSVYSTRTYSTI"
     gene            1195..>2184
                     /locus_tag="VN039_01870"
     CDS             1195..>2184
                     /locus_tag="VN039_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWV44763.1"
                     /translation="MTFTPVPFYILCGSLGAGKTTLLMRLLEHWKTQGRRTGVLMNEA
                     GAVSIDGPRAGTLAEQVMNLAGGCVCCDTKEDLCWGIAQLVHDHGSDMLILECSGLAD
                     PAEVIDAVTDAYTARLASLERIMALLHPVSTEESHSSAYVTTQAIRCADELILNKRDL
                     YVPGHWERFRRSILDQNPYARLWETSHAHLDTVQLLAPHPSAKHTPPTNVVFDKSRSG
                     SSQRAAYHPIATTIPLPGPLNRRRFLAWITTLPKELERAKGYFRFTQEPELQEFQYAL
                     PGQATIAPLTLLDEPEPALVLIGRGYDVERLRAELLATVEHRDFMSIRTARPLP"
BASE COUNT          516 a          607 c          575 g          486 t
ORIGIN      
        1 acgttgccgc cggccagcga cgcaacttcc atcccttgta caagacaact ccgatcgcca
       61 cagccaccac ctttttctat aggaacctag tcctctttgt aacgatgaag acctcaacgg
      121 cgttcaaagc ctagaacccg ccgacgcgtc tcttccgatt gattaccagc tcaatcgctt
      181 caaattgtcg aatatgtgcg agtgctatac acagaaaaga cttcggataa ctgttcccgt
      241 tgggggccgg gatcgacgag gtgggtcata gcctctgaaa ggacacggat cagttttcca
      301 tgttcgactg gtttgggcag gtatgcatag actccccgat ttatagccag gtcaacgaca
      361 tcttcgtaat ggtctgacat gagaatgatc ggcagttgcg gccacaccag ctgacattgg
      421 tagaggaggg cgagaccatt caaattaggt gtctgcatat cggcgatcac ggcatcaaaa
      481 tgacggcgcc ggagttcgct caagacccgc agtccatccg cgaccgccac caccacaaag
      541 tggtgcaggt ccagttcgcc aatgaggaag ctgtctacga actcttcatc accgataact
      601 aatacccgct tgccataacc aatcatcgaa tgctccttcc gtcaggagtg attcggagtt
      661 tccttgatgt gagaccgaga cggaatcccg atacagtact cccgcttcct tcgggattcc
      721 aaccggagaa gaccttatag actggtcgag gcatggggct gactagttgt gagcaaaatt
      781 gatgccttgc cgttagctct ccgtagtctg tatggttatt atcatgtggc gagcgaggtg
      841 cggcgatcgt taacacctgc aaggtatgtt aaaaaagcat gatgctgctg tgcgattctg
      901 cagcgagcac cctgtgcgaa agacaccaag ggaatagggc tgaagtgctg ggtaacagtc
      961 gaccgatggc tagttgaaat gtcataagtg aaaggtgctg tctgattgtc tacgatccaa
     1021 gacaggaaaa ctgatcgtac gcatcgggac gatcagcaac caaaagtcta ccgctaatcc
     1081 cgccgccaga catacacttg aacgctcaat cgatgaatct agcggtttct ggaagcaacc
     1141 cttcatgttc tagacttatc ccggtacaat ggggtgcgtt gattgaggac aagtatgacc
     1201 ttcactcccg tccccttcta tatcctctgc ggctctctcg gcgccggaaa gacgacactg
     1261 ttgatgcggc tgctggaaca ttggaaaacc cagggccgac gaaccggtgt actgatgaac
     1321 gaggccgggg ctgtcagtat tgatggccct cgcgccggca cgcttgccga gcaggtcatg
     1381 aatcttgccg gaggttgtgt ctgttgcgac acgaaggaag atctctgctg gggcatcgcg
     1441 caactggtgc acgatcatgg atcggacatg ctgattctgg aatgctcagg tcttgccgat
     1501 ccggctgaag tgatcgatgc ggtgactgat gcctatacgg cgcgattggc ttctcttgag
     1561 cgaatcatgg cgcttctgca tccggtctcg acggaggaaa gccattccag cgcgtatgtg
     1621 acgacgcaag cgatccgctg tgccgatgaa ctcatcttga acaagcgaga cctctacgtc
     1681 cccggccact gggagagatt ccgccgttcg atcctggatc agaaccccta tgcgcgtctg
     1741 tgggaaacat cacatgcgca cctcgatacc gtccaactct tggccccgca tccatcggca
     1801 aagcacacgc caccaacgaa tgtcgtattt gacaagtcac gatccggctc ttctcaacgt
     1861 gcggcctacc atcccatcgc cacgacaatc ccgttgcctg gtccactgaa tcgccgacga
     1921 ttcctggctt ggatcaccac cctccccaaa gaactcgagc gggcgaaagg ctatttcaga
     1981 tttacgcagg aaccggagct gcaagaattc caatatgcat tgccgggaca ggccactatc
     2041 gcgcctctta cactgttgga tgaaccagag cccgcgctgg tgctgatcgg ccgaggatac
     2101 gatgtggagc gtctgcgggc tgaactcttg gccacggtgg aacaccgaga ctttatgagc
     2161 attaggaccg cacgtccact tcct
//
LOCUS       DATMTI010000109         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_216836, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000109 DATMTI010000000
VERSION     DATMTI010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216836"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..4227)
                     /locus_tag="VN039_01875"
     CDS             complement(4..4227)
                     /locus_tag="VN039_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV44764.1"
                     /translation="MPQSEPVAILLVNAIAEEIKLATLNFRRFFPNCRVEAVYTVEEA
                     LQWGQRASWQLILIDEDLLAQRAAPVFSELRRLVPYATLVLQTAHSDSAAALNALQAG
                     ADFLLYKQSPAFLAELMLYTKCALETRAIRMTLKQTEDRHGCLIDILSDGLYELDGEG
                     RFVYLSPLATEMLGYTENEVIGTPYSTVVPPDQQDRARHHFNDRRTGTRAARRIEIDL
                     IRKTSSDKPAPTRVRAEISARGLYDFNRRYLGTLGLLRDISHHRRQMKTIDQLEHQLR
                     ESDRLVAIARRFSTLSKNLHAPHNAIQAQSQLLLKTIRDARLIEQVDSLATYAAEAAH
                     LGQELVQTTTEIVTRPDTINDIIETILAAATPPLTLANIEWIERAYEQNLPSFTGSLD
                     SMMDLMRILLTHARRYVVAVGTPHRLRISTRAINEAGDPVAQETSLLPSTAAAEFEIH
                     IQETDIIISAEEPPRQTTTDDLFEAYALIKRLGGRWNFLARVGGFLSIKVWIPVEQPP
                     VSDSPGLSAPLLVSSAGGAVERITETILQTPAHTLPTAPFPSVQPTEPLPDRRKRIRT
                     TLDLPARVTIGNEPYESVVTNLSPSGASLEIDGVLPSFEQQPVYLLIKTAISILELDA
                     IAHDRGETPRRAGIERRASRVALEFTAPNESQQKILTSFIEAVRIHALAITVEAQFPP
                     LHKTGDYVATHTENGLQGTDSRETVRVRVSLPVRIETSEAAAGSLLGIAINLSRGGVC
                     VQTESFLKMTDEEIAIHFSSIGHHDQQRVQKPKAPEAILTGRIVHLAPHPAVSSELTA
                     GPSQPGQRIGIRFSQLTPFAEREVNRMLEQYIGSSVDVEDREGRPLIMSERWECRNTH
                     GQTIAVTADHARREISLDTPIIVVIPGFGSTQTDYVPLSFYLAANHLRVLRYDHSNHV
                     GQSEGSILQITLRNMQTDLQSVLDFVHTTWPAAPVTLLAEDIAARVAVKVMARSTSAD
                     RLFLANPVLDLETALSLIDRSNVIETYRQGHRHGVVNLWGLNVNLDKFIGDAITGEYV
                     DLASSRADFAQLAAPPVILVSPGKYRPIEHMFGPQYESLSTLTPRPTVVSLPTDVSGK
                     SEANDERRTEAFKAILKLISTSLIGDSPSAQVREPSFRDVDRQRQLEHERIRIRYHVS
                     QATRSALWGAYLAHLPQLENLPDYLALTNELYRRLLPLEPGMTILDMGCGQRNFVRLM
                     LTNQAYRSAHQSGPTAVPLHYIGLDQSHESLRLAEGQTHTFAEELPATLTAAVPVGQR
                     LATSWTHTDWNVPFPFTDGSIARILCNLSLSFTPSPIYCLRQMLRVLHPDGTAVVTCV
                     QPHTDLSMLFRRHLCAADRDEFDSPAKVVLHYLGRLREAVRHGLLHTYERDELAGLLA
                     HAGAGSIQFFPVLDNQLLLAIVRKTKSAG"
BASE COUNT          928 a         1069 c         1354 g          960 t
ORIGIN      
        1 gtttcatcca gcggatttgg tcttccgtac aatggcgagg agaagttggt tgtcgaggac
       61 ggggaaaaat tggattgacc ccgcaccagc atgagccagc agaccggcga gctcatcccg
      121 ttcataggta tgcaataagc catggcgaac ggcttcacgc agacgtccga gatagtgaag
      181 gacgaccttg gccggagaat cgaattcatc acggtctgct gcgcacaggt ggcggcgaaa
      241 caacatcgag agatcggtgt gcggttgaac gcaggtcacg acggccgttc catccggatg
      301 caagactcga agcatctgcc tgagacaata aatgggagag ggggtaaagg agagcgaaag
      361 attgcagaga atgcgcgcaa tcgatccatc ggtgaaaggg aaaggtacat tccagtccgt
      421 gtgagtccag cttgtcgcta accgttggcc gactgggacg gcagccgtga gtgtggcggg
      481 aagttcttca gcgaatgtat gggtttgtcc ttctgcaagt ctaagcgatt cgtgcgactg
      541 gtccaacccg atatagtgga gaggaacggc cgtcggtccg ctttgatgtg ccgaccgata
      601 ggcctgattg gtcagcataa ggcggacgaa gttcctttgg ccgcagccca tatcgaggat
      661 agtcatgcct ggttcaaggg gaaggagccg gcgatacagt tcattcgtca aggccaaata
      721 gtccggcaag ttttcgagct ggggcagatg agccaagtaa gcaccccaca aggcgctgcg
      781 tgtggcctgc gagacgtgat agcgaatacg gatgcgttcg tgctccagct gccgttgccg
      841 atccacatcg cggaagctcg gttcccggac ctgagccgag ggagaatcgc cgatcagaga
      901 agttgagatc agcttcaaga tcgccttgaa ggcttcggta cggcgctcgt cgttggcctc
      961 ggattttcca gacacgtcgg tcggcagcga tacaacggtt gggcgggggg tcagggtgga
     1021 gagggactca tattgagggc cgaacatgtg ttcaatgggg cgatactttc cgggagagac
     1081 caggataaca ggcggagcgg ccagttgagc gaagtcagct cttgacgagg ccaagtcgac
     1141 atattctccc gtaatcgcat caccgataaa cttgtcgagg ttgacattga ggccccagag
     1201 attgacaacg ccatgccgat ggccttgccg gtaggtttca atgacattgg agcgatcaat
     1261 aagtgaaaga gcggtttcta ggtcaaggac aggattcgcc aagaaaagcc gatcagccga
     1321 tgtgcttcgg gccatgactt tcacagccac tcgtgcagca atgtcttcgg cgaggagggt
     1381 caccggagcg gcgggccatg tggtgtggac aaagtccaga acgctctgaa gatcagtctg
     1441 catattgcgc aatgtgatct gcaagatgct gccttcgctt tggccgacgt gattcgagtg
     1501 gtcatagcgc aacacgcgca aatggttggc agcaaggtaa aacgagagtg gtacgtagtc
     1561 ggtttgggtc gatccgaatc ccgggatgac gacgatgatc ggggtgtcta gggaaatttc
     1621 acggcgagca tgatcggccg tcaccgcaat ggtctggcca tgcgtattcc ggcattccca
     1681 tcgttcgctc atgatcaatg gccggccctc tcgatcctca acgtctacgg aggagccgat
     1741 gtattgttca agcatgcggt tgacttcccg ttcagcgaat ggcgtcagtt gggaaaaacg
     1801 gatgcctatg cgctgtcctg gttgtgatgg acctgccgtg agctcggatg aaacagcggg
     1861 atggggagcg agatggacaa tgcggccggt caggatcgct tcgggtgctt tcggcttctg
     1921 caccctctgc tgatcatggt ggccgattga agagaaatga atcgcgattt cctcatcggt
     1981 cattttcagg aacgactccg tttgcacgca taccccgcct cgactgaggt tgatggcaat
     2041 gcccaacaga cttccggctg cagcttcaga agtttcaatg cggaccggca gcgagactcg
     2101 aactctcacc gtctcacgag agtccgtgcc ttgaaggccg ttttccgtgt gggtcgctac
     2161 ataatcgccc gtcttatgga gcggcggaaa ctgagcttcg accgttatgg ccaatgcatg
     2221 tattcgtacc gcctcaataa acgacgtgag gatcttctgc tgactctcat tgggtgcggt
     2281 gaactcgagt gcaacccggg aggctcgccg ctcgatgccg gcccgtcgtg gagtttctcc
     2341 tcggtcatgc gcgatggcat ccaattccaa gatactgatg gctgtcttaa tgagcaggta
     2401 gacgggctgt tgttcgaatg acggcagcac accatcgatc tccaatgagg ctccgcttgg
     2461 actcagattc gtcacgacac tttcgtatgg ctcattgccg atcgtgacgc gcgccgggag
     2521 gtcgagtgtc gtccgaatgc gtttgcggcg gtcaggcaag ggctccgttg gttggacgga
     2581 gggaaaaggc gcggtcggca aggtatgcgc cggagtctga agaatcgtct cggtaatccg
     2641 ttcgaccgca ccgcctgccg aggaaacgag taggggcgca gacagaccgg ggctatccga
     2701 cacaggaggt tgttccactg gaatccacac tttgatcgat aggaagccgc cgacccgggc
     2761 cagaaaattc caacgtcctc cgagtcgttt gattaacgca taggcctcaa agagatcgtc
     2821 ggttgttgtc tgccggggcg gctcctcagc cgagatgata atgtctgttt cttggatgtg
     2881 aatttcgaat tcggcggccg ccgttgatgg aagcaaagat gtctcttggg ctacggggtc
     2941 tccggcttcg ttgattgccc tggtactgat tcgtaatcga tggggagttc ctacagcgac
     3001 cacatagcgt cgggcatgtg tgaggagaat gcgcatgaga tccatcatgg aatcgaggct
     3061 tccggtgaaa gatggaagat tctgctcata ggcacgttca atccactcga tgttcgcaag
     3121 agtaagggga ggtgtagcgg cggccagaat ggtctcgata atgtcattga tggtgtctgg
     3181 gcgggtgacg atttccgtcg tggtctggac gagctcttgc cctagatggg cggcttcagc
     3241 ggcgtatgtt gccagcgagt cgacttgttc gatcaggcga gcatcgcgga tggttttgag
     3301 caggagttga gactgtgctt gaatcgcatt gtgaggggcg tgcagatttt tcgacaaggt
     3361 tgaaaagcgc cgtgcgatgg cgacaagtcg atcggattca cgaagttgat gctcaagctg
     3421 atcaatggtc ttcatttgcc ggcggtggtg cgatatatcg cgcaacagac ccagcgttcc
     3481 gaggtaccgg cgattaaagt cgtacagccc tcgcgcactg atctcggctc tgactcgtgt
     3541 aggggccggt ttgtcggagg atgtcttgcg aatgagatca atctctatcc gccgggcggc
     3601 tcgcgtcccc gttcggcggt cgttgaaatg atgacgagcg cgatcctgtt gatccggagg
     3661 tacgacggtg gaatacggag tgccgattac ttcgttctcc gtgtagccca gcatttcagt
     3721 ggccagtggg ctgagataca cgaatcgtcc ttctccatcg agttcatata atccatcgct
     3781 gagtatatca atgaggcagc cgtggcggtc ctctgtctgt ttgagcgtca tacggatggc
     3841 gcgtgtttcg agcgcgcatt ttgtatacaa catcagttcg gcgaggaaag cgggcgactg
     3901 cttgtaaagg agaaaatccg cacctgcctg aagcgcattc aaggcggcgg cggagtcgct
     3961 atgagccgtc tgcagcacga gcgtggcata ggggacaagc ctcctcagtt ctgagaagac
     4021 gggcgcagcg cgctgggcga gcaaatcctc atcgattaag atgagctgcc atgatgcccg
     4081 ttgtccccac tgaagcgctt cctccacggt atagacggct tcgacgcggc agtttggaaa
     4141 gaatcttcgg aagttgagcg tggccagttt gatttcttcg gcgatcgcat tgacgagaag
     4201 gatggcgaca ggctcactct gcggcatggc acgattcccg tgtttctggg caagcctgct
     4261 ctcggggagg tcattcagcg ggctgctgca tatcgccgcg tgtcaatcaa c
//
LOCUS       DATMTI010000110         1754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_131748, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000110 DATMTI010000000
VERSION     DATMTI010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131748"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VN039_01880"
     CDS             complement(<1..1092)
                     /locus_tag="VN039_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HWV44765.1"
                     /translation="MPQEQTQLRDGRIVRSEAPLNLEMPFSSLDSFLTSTKSFYVRTH
                     FPIPVIDRDAWWLYVEGEVEKPFAINYEQLTALESLSAPVTLECAGNNRNFLEPKVKG
                     VQWHLGAVSTADWTGVPLSVLLDRAIPKPNTCEVVLEGADHGILEDPKSPPGELRFAR
                     SVPLGKAYGDVLLAYKMNGEELPPEHGFPVRAIVPGWYAMASVKWLQRIIVVDRPFNG
                     YYQTLDYAYWKRTDYGHWQRGQQIGELTPLTEMQVKAEIARPAEGEMLRANTAVCVHG
                     AAWACDVEIGKVELSTDGGATWNDAKLLGESKPNAWRLWEFSWQTPSQSGKQTLMARA
                     TDSLGRTQPVHRDPDRGTYMINHLLPIEVE"
     gene            complement(1107..1457)
                     /locus_tag="VN039_01885"
     CDS             complement(1107..1457)
                     /locus_tag="VN039_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44766.1"
                     /translation="MDIKALMRSPGSNPQQATIDYSTYDDKTPMLAGGPQYQLQPGTS
                     VIVFADSFDASIPSGYLIQGSRQELLQRVEALRDSLSNMSPDQLKVNEINEDDRRVQM
                     SLYEKLSAALRRAK"
BASE COUNT          372 a          481 c          460 g          441 t
ORIGIN      
        1 ttcaacttcg atcggcagca gatggttaat catgtaagtg ccgcgatcgg gatcgcgatg
       61 caccggttgc gtgcgaccca acgaatcagt agctcgcgcc atgagagttt gtttgcctga
      121 ttgcgacggt gtctgccaac tgaactccca caatcgccag gcgttcggtt tcgattcgcc
      181 cagcaacttt gcgtcgttcc atgtcgcgcc cccgtcggtg ctcagttcga ctttcccaat
      241 ttccacatca cacgcccagg cggctccgtg aacacagaca gccgtatttg cacggagcat
      301 ttcaccttcc gcaggacgcg cgatctccgc tttgacctgc atttcggtta gcggcgttag
      361 ttcaccaatc tgttgtcctc gctgccagtg gccataatca gttctcttcc agtatgcgta
      421 gtccaaggtt tgataatatc cgttgaatgg acgatcgacg acgatgattc gttggagcca
      481 tttcaccgac gccatggcgt accaaccagg tacgatggcg cggaccggga acccatgctc
      541 cggcggcagt tcttcgccat tcattttata agcgagcagg acatcgccgt atgccttccc
      601 taaagggacg ctgcgcgcga accttaattc gcctggcgga ctcttcggat cctcaagaat
      661 gccatgatcg gctccttcca gaacgacttc acaagtgttt ggctttggaa tcgcgcgatc
      721 gagcaggacc gataatggca ctcctgtcca atctgccgtg ctgactgctc cgagatgcca
      781 ctgcacgcct tttacttttg gttccaggaa attccgatta ttgcccgcgc attccaaagt
      841 gaccggagcg gagagtgact cgagtgctgt cagttgttcg taattaatgg cgaacggttt
      901 ttcgacttcc ccttccacgt acaaccacca ggcgtcgcga tcgattaccg ggatcggaaa
      961 atgcgttcga acatagaacg atttggtgga ggtgaggaag ctatcgagcg aggaaaacgg
     1021 catctccaga ttcagcggtg cttcgctgcg cacgattctg ccgtcgcgaa gttgtgtctg
     1081 ttcctgtggc atcggcttgg gtacgcctat ttcgcccggc gcagggcggc agagagcttt
     1141 tcgtacaaac tcatttggac acggcgatcg tcctcgttga tctcgtttac cttgagttgg
     1201 tcgggagaca tattgctgag agaatcgcgt aacgcctcaa cccgctgcaa caattcctgg
     1261 cgactgcctt gtatcagata tccagacgga atgctggcgt cgaaagagtc ggcaaaaacg
     1321 atcaccgaag tgccgggttg caattgatat tgaggaccgc ccgccagcat tggcgtttta
     1381 tcgtcatagg ttgagtaatc gatcgttgcc tgctggggat tgcttccagg cgatcgcatc
     1441 agagctttga tgtccataat tgccttcgct ttgatgtcga acttcgttcg ttgcaaaatg
     1501 tgcacttcct ttaccgaccc aacatagaca acggcggctt tccttcttac cgattcaata
     1561 gagctccgca tattcgcacc gaggagatgc acagggtgtg cacagatcaa aacacacatc
     1621 agaaggaacg tgtatcgtcc gacgcttttc atagggtcac cgtcatgttg ccgatgcagt
     1681 caaataagtg tggccgcttt tacgtcttcc tcagtttccg taggcgacga gagtggttgc
     1741 ggtcggtacg acca
//
LOCUS       DATMTI010000111         1931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_4528, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000111 DATMTI010000000
VERSION     DATMTI010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4528"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1775
                     /gene="groL"
                     /locus_tag="VN039_01890"
     CDS             135..1775
                     /gene="groL"
                     /locus_tag="VN039_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247953.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWV44767.1"
                     /translation="MAKQLMYGDTARAAILKGVNQLADAVKATLGPKGRNAILDKKFG
                     APTITKDGVTVAKEVELKNPYENMGAQLVREVASKTSDTAGDGTTTATVLAQAIFREG
                     AKNITAGANPMEIKRGIDKAVEAVTAELKKLSKPCQSKTEISQVGTISANNDKTIGDL
                     IAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAERMECSVEEPL
                     ILINEKKISSMKDLLPLLEQVAKMGKPLVILAEEVEGEALATLVVNKLRGTLNVAAVK
                     APGFGDRRKAMLEDIAILTGGQVISEDIGIKLENVKLTDLGRAKRVTIDKDNTTIVEG
                     YGDSKKIEGRVKQIKAQIDETTSDYDREKLQERLAKIVGGVAVINVGAATETEMKEKK
                     ARVEDALHATKAAVEEGIVPGGGVAYLRCVKAIDGLKDIPVEQKVGLDIVRRALEEPI
                     RQIAANAGAEASVIVGKVREEKNTNGGYNAATDEYVDMIKSGIIDPTKVSRTALQNAA
                     SVAGLMLTTEVMITELPEEKKEPAMPGGGHSHGMEGMY"
BASE COUNT          507 a          529 c          592 g          303 t
ORIGIN      
        1 aagcttcgga ttgaggatga agagtgcttg atcctcaagg aagaagacat ccttggcgtc
       61 tttaccagct agttcgaacc cctggcagtg ccgatgaact cattcaataa aaaacgataa
      121 caggaggaat ccaaatggca aagcaactca tgtacggcga tacagcacga gccgccatcc
      181 tgaaaggggt aaatcagctc gcagacgccg tcaaggcaac cctcggcccc aagggtcgaa
      241 atgcaattct ggataagaaa ttcggggcac cgaccatcac caaggacggc gtcaccgttg
      301 ccaaggaagt cgagctcaag aacccgtatg agaacatggg cgcccagctg gtccgcgaag
      361 ttgccagtaa gaccagcgac acggcgggcg atgggaccac caccgcgacg gtactagccc
      421 aagccatctt ccgcgagggt gcgaagaaca tcaccgccgg cgccaacccc atggagatca
      481 agcgagggat cgacaaggcc gtcgaggccg tcaccgccga actcaagaag ctcagcaagc
      541 catgtcaaag caagaccgaa atctcgcagg tcgggaccat ttcggccaac aacgacaaga
      601 ccatcggcga tctcatcgcg gaagccatgg aaaaggtcgg taaggacggc gtcatcaccg
      661 tggaagaagc caagtcgatg accacctcgc tggacgtggt cgagggcatg caattcgatc
      721 gcggctacat ctctccctat ttcgtcacca atgcggagcg gatggaatgc tccgtggagg
      781 aaccgctcat cctgatcaac gaaaagaaaa tcagcagcat gaaggacctg ctcccgctgc
      841 tcgaacaagt ggccaagatg ggcaaaccgc tcgtgattct ggcagaagaa gtcgaaggtg
      901 aagcgctggc caccctcgta gtgaacaagc tgcgcggcac cttgaatgtt gccgcagtaa
      961 aggcgccggg attcggcgat cgccgcaagg ccatgctcga ggatatcgcg atcctcaccg
     1021 gcggtcaggt gatttctgaa gacatcggca tcaagctcga gaacgtcaag ctgaccgatc
     1081 tcggccgcgc caagcgcgtc acgatcgaca aggacaacac cacgatcgtg gaagggtacg
     1141 gcgattccaa gaagatcgaa ggccgggtga aacagatcaa ggctcagatc gacgaaacca
     1201 catcggacta cgatcgtgaa aagctgcaag agcggctggc gaagatcgtg ggcggcgtgg
     1261 ccgtcatcaa tgtcggcgct gcaaccgaga ccgaaatgaa ggagaagaag gctcgcgtcg
     1321 aggacgccct gcacgccacc aaggcggcgg tggaagaggg aatcgtcccc ggaggcggcg
     1381 tggcgtatct gcgctgcgtg aaagcaatcg atgggctcaa ggatattccg gtggagcaga
     1441 aagtcggact cgacattgtc cggcgcgcgc tcgaagagcc gatcagacag atcgccgcga
     1501 atgccggtgc agaagcctcg gtcatcgtcg ggaaggttcg ggaagaaaag aacaccaacg
     1561 gtgggtacaa cgccgccacg gacgagtatg tcgacatgat caagtccggt atcatcgatc
     1621 cgaccaaggt gtcgcggacg gcgcttcaga acgccgccag cgtggcgggc ttgatgctca
     1681 ccaccgaggt catgatcacc gaactccccg aggagaagaa agagccggct atgccgggcg
     1741 gcggacacag ccacggcatg gaagggatgt actaaactca gtgctgagtg ctcagcgttg
     1801 agtgctgaga atctggacat gcgaagggct cggcgggaaa ccgccgggcc ctttgtgttt
     1861 tcagagaggc ttgtccgata caattggact catctggaac agggaggaag tctctgctgc
     1921 ttgtcgaggt c
//
LOCUS       DATMTI010000112         1693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_216937, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000112 DATMTI010000000
VERSION     DATMTI010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216937"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1693
                     /locus_tag="VN039_01895"
     CDS             <1..>1693
                     /locus_tag="VN039_01895"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44768.1"
                     /translation="AMVLFPIATPSPKEEPAFVQSLLTRGFTRIKTADDVLDLHEIGK
                     PSLHTSPSLYIILDRLVIREDNRARLVEAIETAFREGEGRCSIDIINHGRQSYSTRFL
                     CQGCGRTFEPLKPILFSFNHPLGACPECKGFGNVLRYDPELVIPDPIKSLAQGVVEPW
                     SKPSSAWWQKQMLLAMKRRGVDTTIPFNSLSKDTQRLLWEGDKSLDGINDFFEYLEGK
                     RYKLHVRVFLSRYRTPSDCPRCHGSRLKSDALFVKISGANIHQVAERTVEGFSEWAES
                     LPLSPFEREIAADIFRQLRAKLGFLQRVGLGYLTLNRQTKTLSGGEAQRVALANQLGS
                     QLVGTLYVLDEPTIGLHARNTDLLAGILHDLADTGNTVVVVEHDRRMIESADYLIELG
                     PHSGEKGGEIVCAAPTQEFIQDRRSTTARYLRGEDHIPLPTKRRSGNGKMLVIAGATE
                     HNLKDLFVRIPLGMLVCVTGVSGSGKSTLVEDTLYRSVARAFRVESLPMGRFKAIKGI
                     EHLSGVRLIDQQPIGRTPRSNPITYLKAFDEIRRLFASEHVAFRQGLTPGHFSFNA"
BASE COUNT          370 a          526 c          435 g          362 t
ORIGIN      
        1 gcgatggtgc tcttccccat tgcgactcct tcgcccaaag aagaacctgc gtttgtccaa
       61 tcacttctca cccgcggatt cacccgaatt aagactgccg acgatgtact cgatttgcac
      121 gagattggga agccctcact ccatacatcc ccctcactct atatcatcct cgatcgcctg
      181 gtaatccggg aggacaaccg cgctcgactg gtcgaagcga tcgaaacggc atttcgcgaa
      241 ggagagggcc gctgttcgat cgacattatc aaccatggac gccagtccta cagcacgcga
      301 tttctctgcc agggttgcgg acggaccttc gaacccctca agccgattct cttttcgttc
      361 aaccatccgc tcggcgcctg tcccgaatgt aagggattcg gcaacgtgct gcggtatgat
      421 ccagagttgg tcatcccgga cccaatcaag tcgctcgctc aaggggtcgt cgagccctgg
      481 agcaaaccca gctccgcctg gtggcagaaa cagatgttgc tcgcgatgaa acggcgcggt
      541 gtcgacacca cgattccgtt caactcgctt tccaaagaca cgcaacgatt actgtgggag
      601 ggggataagt ccctcgacgg cattaatgac ttcttcgagt atctcgaagg caaacgttac
      661 aagttgcatg tccgtgtctt tctcagccgt tatagaacgc cctccgattg cccccgttgt
      721 catggcagtc gcctcaagtc ggatgcgctg ttcgtcaaaa tttccggtgc caacatccac
      781 caagtggcgg agcggaccgt agagggcttt tcagaatggg cagaatcctt acccctatcg
      841 ccctttgagc gagaaatcgc cgcggacatc ttccgacagc tccgagcaaa actgggattt
      901 ctccaacgcg tcggccttgg gtacctcacg ctcaatcggc aaaccaagac tctctccggt
      961 ggcgaggcac agagggtcgc gcttgccaac cagctgggat ctcagttggt cggcacactg
     1021 tacgtcctcg atgaaccgac catcggcctt catgcaagga atactgatct cttggccggc
     1081 atcctccacg atctggctga caccggtaat acggtggtcg tcgtcgaaca tgaccgccgc
     1141 atgattgaat cggctgacta ccttattgaa ctcggccctc actccggcga aaaaggtggc
     1201 gagatcgtct gtgcggcacc tacacaggaa ttcatccagg accgtcggtc gaccaccgcc
     1261 cggtacttgc gcggcgaaga ccacattccc ctaccaacca agcggcgatc cggaaacggc
     1321 aagatgctgg tgatcgccgg cgccaccgaa cataatctca aagatctatt cgtccgcatt
     1381 cccctgggca tgctggtctg tgtgacaggc gtgtcaggat caggcaagag cacgctggtt
     1441 gaagataccc tctaccgatc cgtcgcccgg gccttccgtg tggaatctct tcccatgggg
     1501 cgtttcaagg caatcaaagg gatcgagcac ctcagcggtg tccgcttgat cgaccaacaa
     1561 ccgattggac gtacacctcg atccaaccca atcacgtacc tgaaagcgtt tgatgagatc
     1621 cgccggttgt tcgcgtccga acacgtagcg tttcgccagg gactcacccc aggccacttt
     1681 tcgttcaatg ccg
//
LOCUS       DATMTI010000113         3880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_131970, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000113 DATMTI010000000
VERSION     DATMTI010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131970"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VN039_01900"
     CDS             complement(<1..334)
                     /locus_tag="VN039_01900"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HWV44769.1"
                     /translation="MFTVRLTIAVCAAILWTAVACPAFSLSLDEAKTKGLVGEKSTGY
                     LGAVNPSNAEAQALVDDVNRKRRQAYADIAKRNRTSVQAVETLAGEKAIQNTKLGNFI
                     EGPSGWMKK"
     gene            complement(350..559)
                     /locus_tag="VN039_01905"
     CDS             complement(350..559)
                     /locus_tag="VN039_01905"
                     /inference="COORDINATES: protein motif:HMM:NF025003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HWV44770.1"
                     /translation="MQRGTVQIGVGVSAWVTLLLTVGACTPRVEVAVPEKPITINLNV
                     KIDHEIRLKVDKDLDRVLSKDSGLF"
     gene            complement(568..3366)
                     /locus_tag="VN039_01910"
     CDS             complement(568..3366)
                     /locus_tag="VN039_01910"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HWV44771.1"
                     /translation="MSAQRYQIAVLLGLVALFSCYLLLPLCASYLLVNRLRDYGYSNV
                     ILQLGYPGWAQMRIPVVSFQQDLGEERLLVSLTDAEIQYDLTQMLQGRVSRIVLRDAA
                     IHVLNVQSTEPTAQSGREADQLDDRESPWRLLTAGDLLGSLPILPFDVLQLDHLTIFR
                     EQATGPLRKVTIDGSLTYSGREVGGHLAFRGRDTASYGLVVVGHSASTWSVTLASQRP
                     QAAPIVSLQSQAHPNGSQIQVKGRLQINVQELAPFIALLVPIGPELERVTGEVEINWA
                     GIAAAEAALSSLWDDEGTHLDGTMRVNATLPALKGVPKDIAVAYEGRFGGNATQAEWA
                     ISPGVLLTATINTQPRIIPEAVRLILPHGDQPVRIENRKPVQGTLYWKETPIRTVAQG
                     PLHVTYGRTPGPLVAQFETTRAEGRGNELVLAEGAYRLEGLLPKAITERLSAKEAMGG
                     VQGMVKLARTHIRGVLLPPSSITAKQIGQGTTLISSVTLNLSEPLTVECDLTAIHCSG
                     GTMIATVRAPTVRISGRNIRITRGILRVQEAETTKAAWIAHGTLSVAGVSPEPSAWWP
                     STSDWAIKFSADQSGIKADLRIDTPTHEGFVTARIEQPLQTAQGVMHGVIGPLTFNGT
                     DRRLSKMIIGLSPSTDLIDGMFTATIDASWSSSFRDRTNVIKMVSATANVVAEKLSGH
                     YYDYVVKGLNTTMVVHTQGLESIVMTQPASIFAAAVQSGVDVANLMASYQIRWKLADD
                     LPIVDIRDFQGEVFGGTISNPGFVLDLAKPPVTTTFFLQNLDLAKVLSVEQQKGLQGT
                     GTLNGTLPVTLTSGGLIVKDGLIEAQSPGGIIRYASTPELAKVLSESDHQLQLVAQAL
                     NNFQYSLLRVGVKYGETGMLDLNARLEGRNPDLRNTPPIHFNLTVQEHIPTLLKSLRL
                     VEDIHGTIERKSRRQGPL"
     gene            complement(3569..>3880)
                     /locus_tag="VN039_01915"
     CDS             complement(3569..>3880)
                     /locus_tag="VN039_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44772.1"
                     /translation="IVQLQDGGTSFQAMAVPLTGTMPQLALVLTHTQDGTVLQKADLP
                     TLAAKGGSAAGLNETSPAEQGAAGAGAGTGGNPAKGNCRQGGEATVAQDGLLDMNFCL
                     K"
BASE COUNT          806 a         1176 c         1001 g          897 t
ORIGIN      
        1 atttcttcat ccacccgctg ggaccctcaa taaagtttcc cagcttggtg ttctgaatgg
       61 ctttttctcc ggcgagcgtt tcaacagcct ggacgctggt gcggtttcgc ttggcaatat
      121 cggcataggc ttgccgccgc tttcgattga cgtcatccac caatgcttgg gcttcagcat
      181 tggaagggtt taccgctccc agatagccgg tgctcttctc cccgaccaac cccttggttt
      241 tcgcttcatc caaagacagc gagaaagcag gacatgcgac cgccgtccat aggatcgccg
      301 cacacaccgc gattgtcagc cgtacagtaa acattattcc ctccggcagc tagaacagtc
      361 cgctgtcctt cgacaacact cgatccaagt ccttgtcgac tttcagccga atctcatgat
      421 caatcttcac attgagattg atcgtgatcg gcttctccgg cacagccact tctactcgtg
      481 gcgtacaggc acccacggtc aggagcagag tgacccaagc cgatacgccg acgcctatct
      541 gcacagttcc cctctgcata gtccgcctca taatggtcct tgtcgtctgg actttctttc
      601 gatggtcccg tggatatcct cgaccaaacg gaggctttta aggagcgtgg ggatgtgctc
      661 ctgcacggtc aggttgaagt gaatcggagg agtattcctg aggtccggat tcctgccttc
      721 caaccgagca ttcaggtcca acatgccggt ttcgccgtat ttcacgccga cgcgcagcag
      781 ggaatactga aagttgttga gcgcttgtgc gacgagctga agttgatgat ctgattctga
      841 aagcaccttt gctaattccg gcgttgaggc gtaccgtatg atcccgccgg gagactgtgc
      901 ctctatcagt ccatctttca cgatgagccc acccgacgtg agcgtgacgg gaagcgtccc
      961 gttgagtgtt ccggttccct gaagtccctt ctgttgttcc acactgagaa cttttgcaag
     1021 atcaagattc tgtaaaaaaa acgtcgttgt aaccggtggc ttggccaagt ccagcacaaa
     1081 gcctggattg ctgatcgttc cgccgaatac ttcgccctgg aagtctctta tatcgacaat
     1141 cggcagatca tccgccaatt tccatctgat ctgataggag gccataagat tggcaacatc
     1201 cacaccgctc tgtactgcgg cagcgaagat cgaagccggt tgggtcatca caattgattc
     1261 aagtccctgg gtgtgtacca ccatcgtggt attcaagccc ttcacgacat agtcgtagta
     1321 atggccggac agcttctcag ccacaacgtt cgcggttgcc gataccattt tgatcacatt
     1381 ggtccggtcg cggaagctgc tcgaccagga cgcatcgatg gtggctgtga acatcccatc
     1441 gatcagatcg gttgaaggag aaagtcctat gatcatcttg ctcagccgcc ggtcggtgcc
     1501 gttgaacgtc agcggaccaa tgacgccatg catcactccc tgcgccgtct gcagcggctg
     1561 ctcaatcctg gcggtcacga acccttcgtg cgtcggagtg tcgattcgaa ggtcggcctt
     1621 gatgccgctc tgatcagccg agaacttgat tgcccaatca cttgtggaag gccaccaagc
     1681 actcggctcc ggactcaccc cggcgaccga caaggttccg tgcgcaatcc atgcggcctt
     1741 ggtcgtttct gcttcctgga ctctcaagat acctcgggtg atgcggatgt tccgaccgct
     1801 tattctcact gtcggagccc gaaccgtcgc gatcatcgtt cccccgctgc aatggatcgc
     1861 tgtcaggtcg cattccaccg tcagcggctc ggacagattc agggtgacac tcgaaataag
     1921 cgtggtgccc tgtccgatct gttttgccgt aattgaagat gggggcaaca gcactccgcg
     1981 tatgtgggtg cgcgccagct tcaccatccc ttgaactcct cccatcgcct ctttagctga
     2041 aagccgctcg gtaatggcct tcgggagaag gccctccagc cgataggccc cttcggccaa
     2101 caccaactca tttccacggc cctcggcgcg ggtggtttcg aactgcgcca ccaatggtcc
     2161 cggtgtccga ccgtacgtca catgcaatgg cccttgcgcc accgtacgta taggcgtttc
     2221 tttccaatag agagttccct gcacgggttt cctgttctca atccgcaccg gctgatctcc
     2281 atgaggcagt atcaatcgga ccgcttcggg aataatgcgc ggttgggtat tgatcgtcgc
     2341 tgtcagcaag acaccgggac ttatggccca ctccgcttga gtcgcattcc ctccgaatct
     2401 cccctcgtag gctactgcaa tatcctttgg cacacctttc agagccggta gcgtggcatt
     2461 gactcgcata gtgccgtcca gatgcgttcc ctcgtcgtcc cacaaagagc tgagtgcggc
     2521 ctccgcggct gcaatccccg cccaattgat ctccacttct cccgtaaccc tctccagttc
     2581 cggaccgatc gggacaagga gcgcaatgaa tggagccagt tcttgcacat tgatctggag
     2641 acggcccttg acctgaatct gtgagccatt tggatgcgct tgcgattgca gggaaacaat
     2701 cggcgccgct tgcggtcgct gtgaagccaa ggttaccgac caggtactgg cggaatgccc
     2761 tacgacgacc agaccatagg acgcagtatc acgtccgcga aacgcgaggt gaccaccgac
     2821 ctctctgccg ctataggtca gactcccatc gatcgtcact ttacgaagcg gccccgtcgc
     2881 ctgctcacga aaaatggtga gatgatccag ttgcaacaca tcgaacggga gaatcgggag
     2941 actgcctagg aggtcccccg ccgtcaacaa tcgccacggt gactccctat catcaagctg
     3001 atccgcttct cttccacttt gcgcggtggg ctctgtggac tgcacgttca acacatggat
     3061 tgccgcatcc cgcaagacga tgcgactgac acgcccttga agcatctggg tcagatcata
     3121 ttgaatctcc gcatccgtga gcgagaccag cagtcgttct tcgcccaaat cctgctgaaa
     3181 cgaaaccacc ggaatgcgca tctgcgccca acctggatag ccgagttgga ggatgacgtt
     3241 gctgtaccca taatcacgaa gtctattcac caacagatag gatgcacaca gaggcaacag
     3301 caaatagcag ctaaagagcg ccaccagccc aagcagaacg gcaatctggt atcgttgggc
     3361 cgacatggtt tccctcatcc gccttcgata actcggcaac gtttgtcaaa caaactctca
     3421 cgccctgtca agaggtccaa acggctctgg cgtaggacac ggtccgaacg gactgttttt
     3481 tcgcggtgtt atacaaaaat ggagggcgac cgctgtttta caatgcaccc gctgaagctc
     3541 catcggctgc gagtagtgca gattcgcatc atttcaagca gaagttcatg tcgagcaatc
     3601 cgtcctgtgc gaccgtggct tcgccgcctt gtcgacaatt gcctttagcc ggattccctc
     3661 ctgtcccggc gccggcccct gccgctccct gttctgcggg agaagtttcg tttaggccgg
     3721 ctgcacttcc ccctttcgca gcaagcgtcg gcagatcggc tttctgcagg actgttccat
     3781 cctgggtatg tgtcaagacg agggcaagct gtggcatcgt ccccgtaagc gggaccgcca
     3841 tggcttggaa ggatgttccg ccgtcttgca gctgcacaat
//
LOCUS       DATMTI010000114         7441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_641300, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000114 DATMTI010000000
VERSION     DATMTI010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641300"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VN039_01920"
     CDS             complement(<1..228)
                     /locus_tag="VN039_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HWV44773.1"
                     /translation="MQQYAIILRRRERQQLTLDDLAVHANVHPEVVLQFVEFGLITPV
                     EQNEKELLFDPAAGPRLRTIVRLRRSLGINLA"
     gene            complement(239..1258)
                     /locus_tag="VN039_01925"
     CDS             complement(239..1258)
                     /locus_tag="VN039_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWV44774.1"
                     /translation="MAVKFRDYYEVLGVGKTATEDEIRKAYRALARKHHPDVNPGDKS
                     ADEKFKEINEAYEVLSDSDKRKRYDQLGSNWKAGSDFTPPPGWEARGAGFDGFSATGG
                     QGSASAFSDFFESLFGRRHGGRGGAGFRMRGNDVDAEITLNLEEAHRGAKRNISFDAT
                     ETCPTCGGSGTKDGKTCPTCHGMGAVLRPKSLEVTIPAGVRQGSVIRLAGQGESGGNG
                     ASAGDLLLHVQIQPHKRFETTGDDNIEMAVPVAPWEAALGATIIVPTLDGTVEMKIPP
                     GTQGGKKLRLRGLGLSRRNGGRGDQYVRLKIVNPPDLSTKQRELFEQLAAESHFDPRK
                     SMDGQ"
     gene            complement(1330..1788)
                     /locus_tag="VN039_01930"
     CDS             complement(1330..1788)
                     /locus_tag="VN039_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWV44775.1"
                     /translation="MKTAQDDEIKQLKEQVRQEHGLYLRALADFDNYRRRIEREQANA
                     ASSGKREIILALLEVLDGFELALQHAEDAPSSLSEGIKAVYRRLLTLLEQQKVEPIRS
                     LGEAFDPRIHEAIDSSPSDRYQPGTVIEEIQRGYRRGDDLLRPARVRVAA"
     gene            complement(1821..3734)
                     /gene="dnaK"
                     /locus_tag="VN039_01935"
     CDS             complement(1821..3734)
                     /gene="dnaK"
                     /locus_tag="VN039_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWV44776.1"
                     /translation="MAKAVGIDLGTTNSVVAVMEGGKPTVMINSEGSRLTPSVVGFTK
                     TGERLVGQIAKRQAVLNPENTIYSAKRFIGRRYSEVQSEIKNVPYKVVPGPNDAVRFS
                     IMGKEYSPEEVSALVLRKLADDAAKYLGEKVTEAVITVPAYFNDAQRQATKDAGKIAG
                     LNVLRIINEPTAAALAYGLEKKENEKILVFDLGGGTFDVSILDVGDGVFEVRSTAGDT
                     HLGGDDFDKRVVDWLAEEFQRDQGIDLRKDRQALQRLTEAAEKAKIELSTALETTISL
                     PFVTADASGPKHLEMKLMRAKFDQLTADLVERCIGPVKQALADAKVTEKDIDEVILVG
                     GSTRTPAVQTLVRRLTGGKEPNQSVNPDEVVAVGAAIQAGVLAGEIKGVVLLDVTPLS
                     LGLETLGGVMTKLIDRNTTIPARKSEMFSTAADNQTAVDIHVLQGERELARDNRTLGN
                     FRLEGISPAPRGIPQVEVSFDIDANGILTVTAKDKDTGKEQKVTITGSTQLSKDEIDR
                     MVAEAQRNSEGDKRRREEVEVRNTADSTAYQVETQIRDLGDRVPSNEKARAEQSINSV
                     RQLVKDNSSDVAKLRQLTSDLQQIAHAISSVAYNQATASGGRAAGTGASSPGGTGSGT
                     TDDVVDAEFKETK"
     gene            complement(3757..4143)
                     /locus_tag="VN039_01940"
     CDS             complement(3757..4143)
                     /locus_tag="VN039_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44777.1"
                     /translation="MLDDLDLSELRLVPRVSYYFRYRLHRRDFHNLLVQDRLRGHYTA
                     KPLYGRLTPKGRVDRSAGYNGDVAALFVPIEAQTVHDASLILTHIDPQRVTLATGRRN
                     WPAIREAAEHGIWEMLAASSAFGRGF"
     gene            complement(4181..4987)
                     /locus_tag="VN039_01945"
     CDS             complement(4181..4987)
                     /locus_tag="VN039_01945"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HWV44778.1"
                     /translation="MNNLHRELAPISDKAWAQIEEEASRTLKRHLAARRVVDVQGPKG
                     FELSAVGTGHLHQIQAPGDGIQAAQREANALVELRVPFELTRQAIDDVERGATDSDWL
                     PLKDAARKIAFAEDYAVFDGYVAAGIQGVRQGSSNLPVTLPSNVRGYPGAVAQAVSQL
                     RLAGVNGPYALLLGAEPYTAVSGGTDEGYPLLEHIQRLVDGEIIWAPAIEGGLVLTTR
                     GGDFELDIGQDISIGYVSHSKTIVELYLQETFTFRLLTTEAVVVLVPGAK"
     gene            complement(4980..6047)
                     /locus_tag="VN039_01950"
     CDS             complement(4980..6047)
                     /locus_tag="VN039_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HWV44779.1"
                     /translation="MPSAVPQPVVAPLTRAAIFLVVTVNSGQNNEAAVRTLCGNLAAL
                     LRAVGFRDLDGRLSCVMAFGSDAWDRLFGAPKPKELHPFREIHGRHHAVATPGDILFH
                     IRSKKRMDLCFELATQIMSRLDGAISAVDEVHGFNYFDDRDLIGFVDGTENPVEQAAV
                     DATIIGEEDAAFAGGSYVIVQKYLHDVKGWNALPVEEQERIIGRTKLSDVELDDAVKP
                     TSAHNALTTIIEDGKQLEILRDNMPFGDVGKGEFGTYFIGYARSPRRIEQMLVNMFVG
                     RPPGNYDRLLDFSRAVTGTLFFVPSATFLENVATVPSSTGPTSKERDAEDKSTSRLSP
                     PDGSLGIGSLKKEAGKEAGYE"
     gene            complement(6182..7342)
                     /locus_tag="VN039_01955"
     CDS             complement(6182..7342)
                     /locus_tag="VN039_01955"
                     /inference="COORDINATES: protein motif:HMM:NF005750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HWV44780.1"
                     /translation="MAKILCVLYPDPVTGYPPTYARDEIPTITTYANGQTAPAPKGPL
                     GFKPGELVGSVSGELGLRHYLEKLGHELIVTSDKDGPNSEFEKLLPDAEVVISQPFWP
                     AYLTKERIAKAKKLKLALTAGIGSDHVDLEAAARAHITVAEVTGSNSISVAEHVVMMV
                     LSLVRNYLPSHEIAIRGGWKIADCVARSYDIEGMHFGTIGAGRIGLAVLRRLKPFDLH
                     LHYTQRHRLDPAIEKELSLTFHPDVESLVRAVDIVNLQVPLYPSTEHLFDDKMFARMK
                     RGTYVINCARGKLVERDAVVRALESGHLAGYAGDVWFPEPAPQDHPWRTMPFNGMTPH
                     MSGTSLSAQTRYAAGTLEILESYFGGTPIREEYLIVDGGRLAGTGAKSYKLT"
BASE COUNT         1448 a         2275 c         2046 g         1672 t
ORIGIN      
        1 cgcaagattg atgcctaaag accggcgaag ccggacgatg gttctcaagc gagggcccgc
       61 cgcaggatcg aacaacaatt ctttttcgtt ttgctccacc ggagtgatca atccgaattc
      121 cacgaactgc aaaaccacct caggatggac attcgcatgg acagcaagat cgtcgagtgt
      181 caattgctgg cgctcccggc ggcgaaggat aatcgcgtat tgttgcatct tccccatcct
      241 actgcccgtc catcgacttg cgaggatcaa aatgagactc ggcagcaagc tgctcaaaga
      301 gttctctttg cttggtgctc aaatccggcg ggttgacgat ttttagcctg acatattgat
      361 ctcctctgcc cccgtttcgc ctgcttaatc ccaggcctcg taaccgaagc tttttacctc
      421 cttgtgtacc aggcggaatt ttcatctcca ccgttccgtc gagcgtgggc acgatgattg
      481 tagccccgag cgctgcctcc caaggcgcga ccgggaccgc catctcaata ttgtcgtcgc
      541 cggtcgtctc aaatcgtttg tgaggttgaa tttgtacatg gagcaagaga tctccagccg
      601 aagccccgtt tccaccggat tcgccttgcc ctgccaatcg aatcacggag ccctgccgca
      661 cgccggccgg aatcgttact tccaaggact tcggacgaag gaccgcaccc attccatggc
      721 aggtcggaca agtcttgccg tctttcgtcc cgctgccgcc gcaggtcgga catgtttccg
      781 tggcgtcaaa gctgatgttg cgctttgctc cgcgatgtgc ttcttcgagg ttcagtgtaa
      841 tttccgcgtc aacatcgtta ccgcgcatcc gaaatccagc gcccccgcga ccaccatggc
      901 gcctcccaaa gaggctttcg aagaagtcac taaaggcgct tgctgaacct tggccgccag
      961 tggcgctaaa accgtcaaat cctgcgcccc gagcctccca gccaggcggc ggggtaaagt
     1021 cagatcctgc tttccagttc gatccgagct gatcgtagcg cttgcgctta tccgaatcgg
     1081 ataagacctc gtacgcctcg ttaatctctt tgaatttctc gtcagcggat ttgtctccgg
     1141 gatttacgtc cggatgatgc tttcgtgcca gcgcccggta cgctttgcgg atttcatcct
     1201 ccgttgccgt tttgccgacg ccaagaacct catagtaatc tctgaacttg acagccatcg
     1261 gaattcactt gaatcggtat gaacctcggc ggatcattcg tctggacgcc tggccaatga
     1321 aacacatcac taagccgcaa cccgtacgcg cgcgggtctg agcaagtcgt caccgcgacg
     1381 atagccgcgc tggatctctt ctatcacggt tcccggctga taccggtcac tcggtgacga
     1441 atcgatggct tcatgtatgc gaggatcaaa agcttcgccg agactccgaa tcggctcgac
     1501 cttctgctgc tccaacaagg tcaacaacct gcgataaacg gccttaattc cttcggacaa
     1561 agacgaaggt gcgtcttccg cgtgctgaag agccagctcg aagccgtcga ggacctccag
     1621 cagagcgagg ataatctcgc gcttgccgct ggaggcggca ttggcctgtt cacgctcgat
     1681 ccgccgccgg taattgtcaa aatccgccaa ggcgcgcaaa tacaatccgt gttcctggcg
     1741 aacttgctct ttgagttgct tgatttcgtc gtcttgtgct gttttcatac cagctgtggt
     1801 cctcttggcc gaggccgccc tcatttcgtc tctttgaatt cggcatcaac gacatcgtct
     1861 gtagtccctg atcctgtgcc acctggcgat gacgcgccgg ttcccgcggc ccggccaccc
     1921 gatgccgttg cttgattgta agctacagac gatatcgcgt gagcaatctg ctgcaggtcg
     1981 ctggtaagct gacgaagttt agcaacatcg gatgagttat ccttcaccag ttgacgaact
     2041 gagttgattg actgctcagc ccgggccttc tcattcgacg gtacgcgatc tccgaggtcg
     2101 cgaatttgcg tctccacctg ataggccgtc gaatccgcgg tgttccgcac ttcgacctct
     2161 tcgcggcgcc gtttatcccc ttccgaattc cgctgagctt ccgccaccat ccggtcaatc
     2221 tcgtctttgc tgagctgagt agatccggta atggtgacct tctgctcttt gccggtatcc
     2281 ttatcttttg ccgtgactgt caggatgccg ttggcatcga tgtcgaagct gacttccact
     2341 tgagggattc cccgcggagc aggggaaatg ccttccagcc ggaagttccc gagcgtccga
     2401 ttgtcgcgcg cgagctctct ctcaccttga agcacgtgta tgtccacagc cgtctgatta
     2461 tctgcagccg tagagaacat ctcagacttc cgagccggta tcgttgtatt gcggtcgatc
     2521 agcttcgtca ttacgccgcc gagcgtctcc aatcccagtg agagtggagt gacatcgagc
     2581 aggaccacgc cctttatttc tcccgccaga acgccggcct ggatggcggc gcccaccgca
     2641 accacttcat ctgggttaac cgactggttt ggttcttttc caccggtcaa gcgacggact
     2701 aacgtttgta ccgccggagt gcgcgtggag ccgcccacta gaattacctc gtcaatatcc
     2761 ttctctgtaa cctttgcatc cgccagtgct tgcttcaccg gcccaatgca tcgctccaca
     2821 aggtcggctg tcaattgatc gaacttggcg cgcataagct tcatttccag gtgcttcggt
     2881 ccggaggcat cggctgtaac aaacggcagg ctgatcgttg tctcaagcgc ggtcgatagt
     2941 tcgattttcg ccttctctgc cgcttcggtc agccgttgca acgcctggcg gtccttcctc
     3001 aaatcgatcc cttgatcgcg ctggaattcc tcggccagcc agtccactac ccgtttatcg
     3061 aaatcatctc cacccaggtg agtgtccccg gcagtcgaac gaacctcgaa gaccccgtcg
     3121 ccgacgtcga ggatcgaaac atcgaaggtt cccccgccaa gatcgaacac taaaatcttc
     3181 tcgttctctt tcttctcgag cccataggcg agcgcggcag cggtcggctc gttgataatt
     3241 ctgagcacat tcagtccggc gattttgccc gcgtccttgg tcgcctgcct ctgggcgtcg
     3301 ttgaagtatg caggcacagt gattaccgct tcagtaacct tttctcccaa gtatttggct
     3361 gcgtcgtccg ccagctttcg caacactaga gccgaaactt cttccggtga gtactctttg
     3421 cccatgatcg agaaccgaac ggcgtcgttc ggccccggca cgactttgta ggggacattc
     3481 ttaatctcgg actgaacctc ggagtagcgg cgcccgatga acctctttgc cgaataaatc
     3541 gtattttccg ggttcagcac ggcctggcgt ttcgctatct ggccaaccaa gcgctcgcct
     3601 gtcttggtga acccgacaac cgaaggcgtt aaccggctcc cctcgctgtt gatcatgacg
     3661 gttggcttac cgccttccat aaccgcgact acggagtttg tcgttcctag atctatgcct
     3721 actgcttttg ccataacgtc ttacctcctg ttttcctcaa aacccacgtc cgaatgcgga
     3781 ggatgcagcg agcatctccc agatcccatg ttccgcggct tcgcggatcg cgggccagtt
     3841 gcgccgacct gtggccagtg tcacgcgctg gggatcgatg tgtgtgagga tcaagctcgc
     3901 gtcgtgaacc gtttgggctt cgatgggaac gaagagagca gccacatcgc cattgtatcc
     3961 ggcagaacgg tcgacccgac ccttcggtgt gaggcggccg tagagcggct ttgccgtgta
     4021 gtggccccga agccgatcct gtacaaggag attgtgaaag tcgcgtcgat gcagtcgata
     4081 gcgaaaataa taggaaactc gcggaaccag ccgcaactcg ctgagatcga gatcgtccag
     4141 catcactcag ctcccaatcc cggatggatg tgcccaacgt tcactttgcc ccaggcacca
     4201 ggaccacgac agcctcggtc gtcagcagtc ggaacgtgaa ggtctcctgc aagtacaatt
     4261 cgacgatcgt cttggaatgg ctcacgtaac cgatagagat gtcctgcccg atgtctaatt
     4321 cgaaatcacc gccacgggtc gtcagcacca atcccccttc gatggccgga gcccaaatga
     4381 tctctccatc gacaaggcgc tgaatatgtt cgaggagggg atatccttcg tcggtccctc
     4441 cgctgacggc cgtgtacggc tctgcgccca gcaagagagc gtaggggcca ttgacgccgg
     4501 cgagccgcaa ttgactcacc gcctgcgcga ccgcaccggg ataacccctc acattggaag
     4561 ggagcgtcac cggcagattg ctgctgcctt gacggacgcc ttgaattcca gcggcaacat
     4621 acccgtcgaa gacagcgtaa tcttccgcga aggcgatttt gcgcgccgca tccttcagcg
     4681 gcaaccagtc cgaatccgtt gcgccgcgtt ccacgtcgtc aatcgcctgg cgcgtgagtt
     4741 cgaacggaac gcgcaattcg acgagcgcgt tcgcctcgcg ctgtgcagcc tggatcccat
     4801 cgcctggtgc ttgaatctgg tggagatgac cggtgccgac agccgagagt tcgaagccct
     4861 tcggaccctg cacgtctacg acccgccgcg ccgccaagtg ccgcttgagg gtgcgagacg
     4921 cctcctcttc gatttgagcc caagctttgt cggagatagg ggcgagttcc cgatgaagat
     4981 tattcatatc ctgcctcttt tcctgcctct tttttcagcg aaccaatacc gagtgaaccg
     5041 tcgggcggag acaaccgcga tgtggatttg tcctcagcat cacgttcttt cgaggtcggg
     5101 ccggtactcg atgggactgt cgcaacattc tccaggaacg tcgccgacgg cacaaaaaac
     5161 agcgtgccgg tcacggcgcg gctgaagtcc agcagccggt catagttgcc tggtggccgg
     5221 ccaacgaaca tgttcacgag catctgctcg atcctgcgtg gcgaccgggc gtagccgatg
     5281 aaataggtcc cgaattcccc ttttcccacg tccccgaacg gcatgttgtc gcgcagaatc
     5341 tccagttgct ttccgtcttc gataatcgtc gtcaacgcgt tgtgtgccga ggtcggcttc
     5401 acggcatcgt cgagttcgac gtcggacagt ttggttcggc cgatgatccg ctcctgttct
     5461 tcgacaggga gcgcgttcca gcccttcaca tcgtgaaggt acttctggac gatcacgtag
     5521 ctcccaccgg caaacgcggc atcctcctcg ccgatgatgg tggcgtcgac agcggcctgt
     5581 tccaccggat tctccgtccc atcgacgaat ccgatcaggt cgcggtcatc gaagtagttg
     5641 aaaccgtgca cctcgtccac cgcggaaatg gcgccgtcga gccgcgacat gatctgtgtc
     5701 gccagttcga agcagagatc catgcgcttc ttggaccgga tatgaaagag gatgtcaccc
     5761 ggcgttgcga cagcgtgatg gcggccgtgg atctcccgga acggatgcag ttctttcggt
     5821 ttcggcgctc caaagagtcg gtcccatgcg tcggagccga acgccatgac gcatgacagc
     5881 cgcccatcaa ggtcacggaa gcccactgca cgcaggagcg cggccaaatt accgcacaag
     5941 gtccgcaccg cagcttcatt gttctgtccg gagttgacgg tcacgacgag aaaaatagcg
     6001 gcccgcgtga gcggggcaac gacgggttgt ggaacggcgg atggcatacg tgactcctta
     6061 ctgacctgtt cgtgggtcct tcgtgtaaag cgcgaccacc tcggtcggcg aaagccgccg
     6121 gtcgcccgag ttgcactgtc gttcgtcgat gtaagatgcc ggcatcacgt cccatttccg
     6181 atcaagtcag cttgtaagac ttggcacccg tgccggcgag tctcccgccg tccacgatga
     6241 gatattcctc acggatcggg gttcctccga aataactctc caagatttcc agcgtgccgg
     6301 cggcataccg cgtctgcgcg gagagcgacg tgcccgacat gtgcggcgtc atgccgttga
     6361 agggcatggt tcgccagggg tggtcctgcg gcgccggttc cggaaaccac acatcaccgg
     6421 catagccggc gaggtgaccg gactcgagcg cgcgcaccac ggcgtcacgc tcgaccagct
     6481 ttccccgcgc gcagttgatc acgtacgtgc cgcgcttcat cctggcgaac atcttgtcgt
     6541 cgaacagatg ttcggttgag ggatagagcg gcacttggag gttgacgata tcgacggcgc
     6601 ggacgagcga ttccacgtcg gggtggaagg tcaggctcaa ctccttctca atcgccggat
     6661 ctagacggtg gcgttgagta taatgcagat gcaggtcgaa gggtttgagc ctacgcagca
     6721 ccgcgagacc gatcctgccc gcgccgatcg tgccgaagtg catgccttca atatcgtagc
     6781 tgcgggcgac gcagtcggcg atcttccatc cgccgcgaat ggcgatctcg tgcgacggca
     6841 ggtaattgcg gaccagcgac agcaccatca tcacgacatg ttccgccacg ctgatgctgt
     6901 tggagccggt tacttcagcg acggtgatat gcgcgcgggc ggccgcctcg agatcgacgt
     6961 gatcagagcc gatgccggcg gttagggcga gcttcagctt cttggccttg gcgatccgct
     7021 ccttggtcag ataggccggc cagaacggct gggaaatgac cacttccgcg tcaggaagaa
     7081 gcttctcgaa ctccgagttc gggccatcct tgtcgctggt cacgatgagc tcatggccga
     7141 gcttctccag atagtggcga agacccagtt cgcctgagac cgacccgacg agctcgcccg
     7201 gtttaaatcc gagaggcccc ttcggtgcag gcgcggtttg gccgttggca taggtggtga
     7261 tagtcgggat ctcatcccgc gcataggtcg gcggatatcc agtgactggg tctggataaa
     7321 ggacacataa aattttcgcc atgggagtac gtttcctttc cttctcctgg ctgcacactg
     7381 tacggttgcc aatgacatat agaagtcaag acttcatctg acagacagtg cgccctcacc
     7441 a
//
LOCUS       DATMTI010000115         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_132092, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000115 DATMTI010000000
VERSION     DATMTI010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132092"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VN039_01960"
     CDS             <1..346
                     /locus_tag="VN039_01960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWV44781.1"
                     /translation="VIPGFMIQGGDPNTKDTLKKDIYGQGGPGHAIKAEFSDIPHKRG
                     IVSMARATDPDSAGSQFFIVVEDSRFLDRKYSVFGEVTKGIGVADKIVNLPRDERDNP
                     RERVEMTVTIVE"
     gene            complement(390..1292)
                     /locus_tag="VN039_01965"
     CDS             complement(390..1292)
                     /locus_tag="VN039_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWV44782.1"
                     /translation="MSLPDSLNVPILRNIFHPSDFTPASEAAFAHALRAALMAQANLT
                     ILHVSPDRDGEWMEFPGVRAALERWGLLPMNSRRSDVAQLGIHVKKVRAIHPDPIESV
                     TSYLAEHATDLIVLATDQSNGCLPWLSKSVAVPVALESRQMTLFIPKGAAGFVSAHDG
                     SVSLTNILIPVASKPPGQAALQAAVRMAYRLNRSVGVFTVLHVGEEGDMPDLHYSDMP
                     GWQWEKVIKQGDVVDVICGTAEEMKADLIVMTTEGRHGFLDALRGSHSERVLHKTPCP
                     LLTIPAGGFIASVLEEESESRQAP"
     gene            complement(1478..2008)
                     /locus_tag="VN039_01970"
     CDS             complement(1478..2008)
                     /locus_tag="VN039_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44783.1"
                     /translation="MQFASLRRDFYQELDQLGGHKAVAFFNLIEVITFGYFERRRDGQ
                     DVEVITALQTLRRALSPLHVPAGPAPVFAEHLKKEYELFRKQNPEEMADAADALVVLD
                     RAAQFVSAFSENDFQSSRFLGGLIGYVKMEHPEIAAHLKKQREAGHILLPGQSFLPTP
                     SAETHTHGPDCRHHHN"
     gene            complement(2176..>2400)
                     /locus_tag="VN039_01975"
     CDS             complement(2176..>2400)
                     /locus_tag="VN039_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44784.1"
                     /translation="HRFQVIAEYLPAPADISIDRVNNLILVPYHYMHAAEINGLETPS
                     SAKPKGEKRTLADYGFIPPPPKPGSEGTKK"
BASE COUNT          509 a          646 c          719 g          526 t
ORIGIN      
        1 tgtcattccc ggtttcatga ttcaaggcgg agatccgaat acgaaggaca cacttaaaaa
       61 ggacatctat gggcagggtg gccccggcca tgccatcaag gcggaattca gcgatattcc
      121 gcacaaacgc ggcattgttt caatggccag ggcgactgat ccggattcgg ccggctccca
      181 gttttttatc gtcgtagagg actcacgatt tctggaccgc aagtactccg tgttcggcga
      241 agtgacgaag ggaatcggtg tggccgacaa gatcgtcaat ctgccacgcg atgagcggga
      301 taatccgaga gagcgggtgg aaatgacggt cacgatcgtg gagtagtttc cctcgatatt
      361 tgtgctggag gctcatgtgc ggcgcgtctt caaggtgcct gtcggctttc tgattcctcc
      421 tccagcaccg aggcaataaa tccgcctgct ggaattgtca gcagcgggca gggcgtcttg
      481 tgaagcaccc gctcgctatg gcttccccgt agcgcatcga gaaaaccatg gcgtccttcg
      541 gtcgtcatga cgatgagatc cgccttcatc tcctcagcgg tcccgcagat cacgtccacc
      601 acatctccct gcttgatgac tttctcccac tgccagccgg gcatatcgga gtagtggagg
      661 tcgggcatgt ccccttcctc gccgacatgt agaaccgtaa agacgccgac cgagcgattg
      721 agccggtatg ccatccgcac ggcggcctgg agtgccgcct ggccaggcgg cttggaggcg
      781 acgggaatca agatgttcgt caacgagacg gaaccatcgt gcgcggaaac gaatccggct
      841 gcacctttcg ggatgaagag ggtcatctgt cgcgactcca gcgcaaccgg caccgccacc
      901 gacttgctga gccatggaag acagccgttg ctctgatcgg tagcgagcac gatcaaatcc
      961 gtcgcgtgtt cggctaagta agatgtgacc gattcaatgg gatccggatg gatcgctcgg
     1021 accttcttga cgtggatacc caattgggcg acatcggatc gccgactgtt catgggcagc
     1081 aggccccatc gctccagcgc cgcacgtaca ccgggaaact ccatccattc cccatcccga
     1141 tcgggggaaa catgaaggat ggtcaggttg gcttgagcca tcaaggcggc tctcaaggca
     1201 tgagcgaacg cggcttcgct tgcaggagta aaatcggatg gatgaaagat attccgaagg
     1261 atagggacgt tgagtgagtc gggaagagac ataggcagcc cccctcggac ggctagcata
     1321 tcatgcaggt gcttcgactt gcactggggg aaatccccgg gctgatggac gggttcgcgc
     1381 ctgctgtctt ccgcgtatag gcctgctcct agatggaggt cagccttgca ccggccggca
     1441 atttccttga gcgatcgggc gctgcaccat aagtgagcta attgtgatgg tgtctgcagt
     1501 cgggaccatg agtgtgagtt tcggcggagg gtgtcggcag gaaagattgt cctggtaaaa
     1561 gaatgtgtcc cgcctcacgt tgcttcttga gatgggccgc gatctccgga tgctccattt
     1621 ttacataacc gatcagccct ccaagaaatc ggctcgactg aaaatcattc tctgaaaatg
     1681 cggagacaaa ttgcgccgcg cgatccagga cgaccagcgc gtcggcggca tcggccattt
     1741 cttcgggatt ctgtttccga aacaactcat actccttttt gagatgctca gcaaaaaccg
     1801 gtgcggggcc ggcggggacg tggagtggac tgagggcgcg acggagcgtt tgaagtgccg
     1861 tgatgacctc gacatcctgt ccatcccttc gtctttcgaa gtagccgaac gtgatgactt
     1921 caatcagatt gaagaacgcc acggctttgt ggcctcccaa ttggtccagt tcttgataga
     1981 agtctcgccg aagcgacgca aattgcacac cgagtctttt ttgttggtaa tcgcttccgc
     2041 tgtcgagata cgtgcaatca ttggggcacg agatccgtgt caggcgatgc tccccacagc
     2101 agagactgca gatcaatcct ccgagggcag ggcacgggcg tttgcccttt cgacggtggc
     2161 aatacgtgca cgagctcatt tcttcgttcc ttccgacccg ggcttgggag gcgggggaat
     2221 gaatccgtaa tcggctagtg tgcgtttctc gcccttaggc tttgcggatg aaggggtttc
     2281 aagtccattt atctcggcgg catgcatata gtggtaggga accaagatca gattgttcac
     2341 gcggtcgata ctgatgtcgg ccggagcggg gagatactcg gcgatgactt ggaaccgatg
//
LOCUS       DATMTI010000116         1640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_556650, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000116 DATMTI010000000
VERSION     DATMTI010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556650"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VN039_01980"
     CDS             <1..214
                     /locus_tag="VN039_01980"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="catalyzes the conversion of dihydroorotate to
                     orotate in the pyrimidine biosynthesis pathway; subclass
                     1A is a dimer formed by two identical PyrD subunits each
                     containing an FMN group; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HWV44785.1"
                     /translation="KIPIIGTGGIVEGIDIFEHFLCGASAVQIGTVLVEEGLDVFGRL
                     EAELTAVLTRKGYRSILESRGRLKEL"
     gene            complement(217..495)
                     /locus_tag="VN039_01985"
     CDS             complement(217..495)
                     /locus_tag="VN039_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV44786.1"
                     /translation="MARNKHTAALPLRRMEQRPKSSLHITPRQREILRLVALGHTNRE
                     IAASLDISVRTVEVHRFNLMRRLNVRNVAQLLRQALQHNLLPRNFGNK"
     gene            617..>1640
                     /locus_tag="VN039_01990"
     CDS             617..>1640
                     /locus_tag="VN039_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWV44787.1"
                     /translation="MEVSLTLMPAFLRVLLLLLTSHVRQWPLRTLLTIVGVALGVSAS
                     VAVRTANVDVLRSFEQTVMTVAGPTTLDVSGGEAGFDEHIITRVRTVVGVTSASPMIL
                     QTAVRMRGEQADQAVQVVGLDLLAEFNTRGFRLSQPASESQLMSMIQPQAVFLGAKLA
                     AEWNLSVDDPVDLLIGPRRLTCRVAGILHNESDRTASWERMAVMDIAAAQITFGMVGK
                     VDRIDIVTDEKRAIEDVAQDLRTVLPSHMTVERPANRTSQVEQMIRAFRLNLTVLSWV
                     GLLVGIFLIYNTMAFAVAQRRREIGIYRAIGMTQSRVAVLFLMEAGLFGFLGGIAGSG
                     AGVALAQ"
BASE COUNT          348 a          380 c          524 g          388 t
ORIGIN      
        1 gaagattccg atcatcggaa ccggaggcat tgtcgagggg atcgatattt tcgaacattt
       61 cctctgcggc gcttccgcgg ttcaaatcgg gacggtgttg gtggaggaag ggctggatgt
      121 gttcggtcgg cttgaagcgg aattgaccgc tgtcttgaca agaaaaggat accggtcgat
      181 tctggaatcc cggggacggc tcaaggaatt atgaatctat ttattgccga agttgcgagg
      241 cagcaagttg tgttgtaggg cctgacgcag gagttgggcg acgttccgaa cattgagtcg
      301 gcgcatcaag ttgaaacgat ggacttcgac cgtccggacg ctgatatcca gtgatgcggc
      361 gatctcgcgg ttggtgtggc ctagcgcgac taatcggagg atctctcgtt ggcgaggcgt
      421 aatatgcaat gaagatttgg gacgctgttc cattcggcgg agcggaagcg cggcggtgtg
      481 tttgtttctt gccatctgta ctcctctcag aaggtctgcg atcagtctag caaacgaccg
      541 gaaatgtgta aacaaaacta ctgatatctg ctgagagtgc gatcacgcag atggggactg
      601 ataagacatc tccaagttgg aagtctctct cacgctcatg cccgcttttt taagggtgct
      661 acttcttctt ctcacctctc atgtccggca atggccgtta cgcacgctgc tgacgatcgt
      721 cggagtggct ctcggagtgt cggcctccgt agccgtacga accgccaatg tcgacgtgct
      781 acggtccttt gagcagacgg tcatgacggt ggccggtccg accaccttgg acgtgtcagg
      841 aggtgaggct ggatttgatg aacacattat tacgagggta cgaaccgtcg tcggcgtgac
      901 gtccgcatcg ccgatgatcc tacagacggc tgttcgcatg agaggagagc aagccgatca
      961 agcggtacaa gtggtgggtc tggatctatt ggctgaattc aatacacgcg ggtttcgctt
     1021 gagccaaccg gcgtcggaaa gtcagctgat gagtatgatt caaccacagg cggtgttctt
     1081 gggagccaaa ttggccgctg agtggaatct atcggttgat gacccggtcg atcttctgat
     1141 tgggcccagg cgtctcacct gtcgggtggc agggattctt cacaatgagt cggaccgaac
     1201 cgcatcatgg gaacgcatgg ccgttatgga tatcgctgcg gcacaaatca cgttcgggat
     1261 ggtcgggaag gtggatcgaa tcgatatcgt gaccgatgaa aaaagggcaa tagaagacgt
     1321 ggcacaggac ctacggactg ttctgccttc ccatatgacg gtggagcgac cggccaaccg
     1381 gacgagccag gttgagcaaa tgatccgcgc cttccgtctc aatctaacgg tgttgagctg
     1441 ggtcggcctg ctggtcggga tattcctgat ttacaacacg atggcatttg ccgtcgctca
     1501 gaggaggcgg gaaatcggaa tttatcgagc gatcgggatg actcagtctc gggtggcggt
     1561 tctgtttttg atggaagcgg ggttgttcgg atttttggga gggatcgccg gaagtggagc
     1621 cggggtggcg ctggctcaag
//
LOCUS       DATMTI010000117         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_47091, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000117 DATMTI010000000
VERSION     DATMTI010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47091"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2396
                     /locus_tag="VN039_01995"
     CDS             <1..2396
                     /locus_tag="VN039_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HWV44788.1"
                     /translation="QDHLGPIPRVQLSLKISALSSRLDSIDPDGSYESVAARLRPLVD
                     LAQSLPAGLIFDMEQAETKDLILHIFKQLFAEPAYRSYPYAGLALQAYHRETERDVQD
                     LVAWVGTRHAPITIRLVKGAYWDSDTVRYRQAGWPSPLFEHKAETDIHYERLISVLFE
                     HSDLIRPAFGTHNLRTLAAIEAHAESRGLTSDAREYQMIFGMAEPFQQAMVKLGRRVR
                     LYTPVGRLLPGMAYLVRRLLENTSNESFLQKEYVESQPLARLLAPPAAPTQFQRTTTN
                     GGKNFSNEPHSDFSQAKVREAMRASVESVQSQLGRQWYGAASGSRLTGPWLESRNPCR
                     PNEVVAQVHSATVADLDHIIERALKQSDPWRRKAPEARSEILRRAASEISRRRYELAA
                     WEICEVGKPWREADADVTEAIDFLRYYADEMDCLARPVRLGNRPGELNQRTYEPRGVT
                     AVIAPWNFPLAIPAGLISAALVTGNPVLFKPSERSPGLGSLLTDILIEAGVPACCLTC
                     LPGGPEVGQALVAHPHVVTIAFTGSKTVGLRILSEASHVRPGQSMVKRVIAEMGGKNA
                     IIVDETADLDEAITGVVASFAGYAGQKCSACSRVIVHRTIYDSFVSRLHEAVLSLEVG
                     DPMYPSTKVGPVIDARAQASIQRYLSLGLEEGRLLVQRSVDQTGYFVGPTVFVDIQPH
                     HRLAQEEVFGPVLAVMKADNIAEAIRLANGTDYALTGGVYARSPANLAMAREQFDVGN
                     LYLNRPITGALVSRQPFGGHRFSGIGAKAGGEDYLAQFMIARVISENTLRRGFESTQ"
     gene            complement(2390..3196)
                     /locus_tag="VN039_02000"
     CDS             complement(2390..3196)
                     /locus_tag="VN039_02000"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44789.1"
                     /translation="MMMKLKCEMRPVLPVLMVGLCLLLGVHPWSVVLGAEEPVTISKS
                     EEYFPDTIGSRWTYRGQITEGPLQSVEQKFFTNVSTVSGTKTMNNVTVTVFHDTNPGN
                     HGASDSFYRRDVVGIVYYGSDPGTPLEKQITPYQIFRFPLRIPSSFQQFDRMGVDFGS
                     DMDRDQTDEKVDVQGWSKVIGRETITVPAGTFQDVIKVEARMNMKIHLSGSRHTVSGI
                     DVMTAWFAKGVGLVKYSERQELSALKEDRGMVTEITEELEDYDIKAAKLH"
BASE COUNT          750 a         1011 c          950 g          765 t
ORIGIN      
        1 aacaagacca cttgggaccg atcccgcgcg tgcagctctc gctcaaaatt tcggccctct
       61 catcccggct ggactccatc gatcccgatg gaagttacga gtcggtcgcc gcacgtctcc
      121 gtccgctggt ggatctggca cagtcgctgc cggccggatt gattttcgat atggaacagg
      181 cggagacaaa agacctgatc ttgcatattt tcaagcaact cttcgcagaa cctgcctatc
      241 gatcctatcc ctatgccggg ctcgcgctgc aggcctatca ccgagaaacc gaacgggatg
      301 tacaggacct ggtggcttgg gtgggaacac gccatgcgcc gatcacgatc cgactggtca
      361 agggcgcgta ctgggactca gacactgtcc gatatcgaca agcaggctgg ccgtctccgc
      421 tttttgagca taaggcggaa accgacattc actacgagcg gttgatctcg gtgttgtttg
      481 aacattccga tcttatccgt cccgcgttcg gcacgcataa tcttcgtacg ttagccgcca
      541 tcgaagccca tgcggaatcg cggggcctga cttctgatgc ccgcgagtac caaatgatct
      601 tcggcatggc ggaacctttc cagcaagcca tggtgaaact ggggcgtcgg gtacggctgt
      661 atacgcccgt gggccggctg ctccccggta tggcctatct ggttcgaagg ctgttggaaa
      721 atacgtcgaa cgaatcgttc cttcaaaaag agtacgtcga atcccagcca ttggcccgac
      781 tcctcgctcc accggcagcg ccgacccagt tccaacgcac gacaacaaac ggcgggaaga
      841 atttcagcaa cgaaccgcac agcgactttt cacaggcaaa ggttcgggag gccatgcggg
      901 caagtgttga atcggttcaa tctcagctgg gccgacaatg gtacggtgcg gcatccggta
      961 gcaggctgac cggcccatgg ctggaatccc gtaatccttg tagaccaaat gaagtcgtag
     1021 cacaagtgca tagcgcaacg gtagccgatc tggaccacat tatcgaacgg gcgctcaaac
     1081 aatcggaccc ttggcggcgg aaagctccgg aagcacgatc tgaaatcctg cgtagggcag
     1141 cgtcggaaat atctcgtcgc cggtacgagc tggcagcctg ggagatctgt gaggtcggca
     1201 aaccttggcg cgaggccgat gccgatgtga cggaagcgat tgattttctc cgctactatg
     1261 cagatgagat ggattgtctg gctcgaccgg tgcgattagg caatcgtccg ggagaactca
     1321 accagcgcac gtatgaacct cgcggcgtga cggcagtcat cgcgccatgg aactttccgc
     1381 tcgccatccc cgccggactg atctcagcgg cactcgtgac cggaaatccg gttctgttca
     1441 aaccgtcgga acgctcgccc ggcttaggga gtctgctcac tgacatactc atcgaggccg
     1501 gtgtaccggc ctgttgtttg acctgcctcc caggtgggcc ggaggtcggc caagcgctcg
     1561 ttgcgcatcc ccatgtcgtg acgatcgcgt ttaccggatc gaagaccgtc gggcttcgca
     1621 ttttaagcga agcatcgcat gtgcgccccg gacagtcgat ggtcaagcgg gtgattgccg
     1681 agatgggcgg gaagaatgcc atcatcgtcg atgagactgc ggatctcgac gaagcgatta
     1741 ccggtgtcgt cgcatcgttc gccggctatg ctgggcagaa atgctcggct tgctcccgag
     1801 tcatcgtcca tcgcactatc tacgactcct tcgtgtcccg cctgcatgag gcggtgttga
     1861 gcttggaggt cggcgatccc atgtacccgt ccaccaaagt aggaccggta atcgatgcca
     1921 gagcccaagc gagcatacaa cgctatcttt cactcggtct cgaagaaggc cggctcctcg
     1981 tgcaacgatc cgtggatcag accgggtatt tcgtcggacc gacggtgttt gtcgatatcc
     2041 aaccccatca ccgcctggca caggaggaag tttttgggcc ggtgttagcc gtcatgaaag
     2101 cggacaatat cgccgaggcg atccgcttgg caaatggaac cgactatgcc ttgaccggag
     2161 gagtttatgc cagaagtcct gccaatcttg cgatggctcg tgagcaattc gacgtaggaa
     2221 acctgtacct gaaccgaccg atcaccggcg ccctcgtctc tcgtcaaccc ttcggtgggc
     2281 accgcttctc agggataggt gcgaaagcgg gtggagaaga ctatctcgcc cagttcatga
     2341 tcgcaagagt catcagcgaa aacactcttc ggcggggatt cgaatcgact cagtgaagct
     2401 tcgccgcttt gatgtcgtag tcttcaagtt cttccgtgat ttccgtcacc atgccgcggt
     2461 cttccttgag ggcggataat tcctgcctct ctgaatactt cacgaggccg actcccttgg
     2521 cgaaccaagc cgtcatcacg tcgattccgg agacggtgtg acggcttcct gacaaatgga
     2581 tcttcatgtt catccgtgct tcaactttga tgacatcctg gaatgtaccg gctgggacgg
     2641 tgatggtttc tcgaccgatc accttactcc acccctggac atcgaccttc tcatctgttt
     2701 ggtcccgatc catatcgctg ccgaaatcca cccccattcg gtcgaactgc tggaaggagg
     2761 acgggattct cagcggaaat cgaaaaatct ggtagggcgt aatctgtttc tccaacggtg
     2821 ttcccggatc cgatccataa tagacgatac cgaccacgtc gcgtcgataa aaactatccg
     2881 atgctccgtg atttcccggg ttcgtatcat gaaacaccgt cacagtgaca ttgttcatcg
     2941 tcttggttcc cgagactgtc gacacattcg taaagaactt ctgttcgacg gattggagcg
     3001 gtccctcggt gatctggccg cgataggtcc agcggctgcc gatcgtatca gggaaatact
     3061 cctctgactt tgaaatcgtg accggctcct cagcaccaag caccacagac catgggtgta
     3121 cccctaagag caagcacagt cccaccatca ataccggcaa taccggtcgc atctcacatt
     3181 taagcttcat catcattcca gctccagaca cgagaagttg acggtatcat aggagtcccg
     3241 agggcggcaa gccgataggt gttgtgttga gtttcgatac ctgcttgtct tggccctctc
     3301 ctcacagcat aatcctctca gatatggaac ctctcatctc atcaatacct tccggaaagg
     3361 atcgaccgac aaaaccgtcc gatcgtccgg cagtcctcat taccggcgct tctggaggca
     3421 tcggtcgcac catcagtcat gcgttcggga aaatcggatg gtatgtgggg gtccat
//
LOCUS       DATMTI010000118         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_641412, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000118 DATMTI010000000
VERSION     DATMTI010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641412"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1335
                     /locus_tag="VN039_02005"
     CDS             70..1335
                     /locus_tag="VN039_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWV44790.1"
                     /translation="MRWFKKILIGLVILSGLTYLYYTEVKPTVIFGLRSDYAHAIPHQ
                     KIPEGLTSLKAESCGTCHTDIYKEWRTTIHAQAYDDPFFQAYWTKDKHTWVCLNCHTP
                     LENQQPTLIKEIPRDRVERAVQGPNPHYDANYQREGVTCAACHVRDGVILGPFEDAKA
                     PHPTKYDPMFRTTQMCYRCHSVVGGPAQFYNGGPCGTYPEFEDGYWSKERGFICQNCH
                     MPEIERPVAVGGPIRQGRQHLWRGGHDPEMIKRAIGVKVVADPAEPKPGDKVRVTLTL
                     VNEGAGHKLPTGDPDRHFTVEFAVEDQNGKVLEDQSDTMGRWIMWQPAIIELHDNRLM
                     PLASRDYTFEYQLPKDRVGLKLIMKVRYHIQSERQHQMLIDKYGLTAKDPYNFTVYER
                     QVPLTGNLADSFEQTGQHTRLACAAPGHN"
     gene            1419..>1609
                     /locus_tag="VN039_02010"
     CDS             1419..>1609
                     /locus_tag="VN039_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44791.1"
                     /translation="MYSTLVVLHILAAVTWIGGMIFLSLVLAPLVRGRKAAPEFMALF
                     RSAALRFRPIVWVAIAVLL"
BASE COUNT          377 a          445 c          449 g          338 t
ORIGIN      
        1 cgaccgataa cagagagaga ggcccatacc ctgtgatgtc gattgtggag cagggaaact
       61 gagagatcga tgcggtggtt caagaaaatt ttgatcggac tcgtcatcct gagcgggctc
      121 acctacctgt attacaccga agtcaaaccg accgtcattt tcggcctacg gtcggactat
      181 gcccatgcca ttccccatca aaagatcccg gaagggttga caagcctcaa ggccgagtcc
      241 tgcggaacct gtcataccga catctacaag gagtggagaa ccaccattca cgcacaggcc
      301 tacgacgatc cgttcttcca ggcctactgg accaaggata aacacacgtg ggtctgtctc
      361 aactgtcaca cgccgttgga aaatcagcaa ccgacgttga ttaaagagat cccgcgagac
      421 cgggttgaac gtgccgtgca ggggcccaat cctcactacg atgcgaacta tcagcgggaa
      481 ggcgtgacct gtgcggcttg ccatgtccga gacggagtga tcctgggacc gttcgaggat
      541 gccaaagccc cccatccgac gaagtacgac cctatgtttc gcaccacgca gatgtgttat
      601 cgatgccata gtgtcgtggg gggaccggcg cagttttata atggagggcc atgtggtacc
      661 tatcctgaat ttgaggacgg ctactggagt aaggagcgcg gtttcatctg tcaaaattgc
      721 cacatgccgg agatcgaacg gccggtcgct gtggggggcc ccatccgcca gggacgtcaa
      781 catctctggc ggggcggaca tgaccccgag atgatcaagc gggcgatcgg cgtgaaagtg
      841 gttgccgatc cggccgaacc taaacccggc gataaggtcc gagtcacgct gacgctggtc
      901 aatgaaggcg caggacataa actcccgacg ggagaccctg atcgtcactt cacggtcgag
      961 tttgccgtcg aggatcaaaa cgggaaggtg ttggaagacc agtcggatac catggggcga
     1021 tggatcatgt ggcaaccggc gatcatcgag ctgcacgaca atcggctcat gccgttggcc
     1081 agccgggact atacgtttga atatcaatta ccgaaggacc gtgtcgggct caagttgatt
     1141 atgaaagtgc gctaccacat ccagtcggaa cgccaacatc agatgctcat cgacaagtac
     1201 ggcctcacgg cgaaggatcc ctataacttc accgtgtatg aacggcaggt tcccttaacc
     1261 ggcaatctgg ctgattcttt cgagcagacc ggacaacata cacgcctcgc ctgcgccgca
     1321 ccaggtcaca actaagaatc acaacaccgg atagagcaaa tcatcttcgc tcattgtctc
     1381 atgttccaac aatagctctc ttcaacgact gaggccgtat gtattccact ctggttgtcc
     1441 tacacatcct tgccgccgtg acctggatcg gcgggatgat ttttctctcc ttggtcttgg
     1501 cgccgttagt caggggccgg aaggcggcgc cggaattcat ggcgctgttt cgatctgcgg
     1561 cgctacgatt tcgccctatt gtgtgggttg ccattgcggt gttactcat
//
LOCUS       DATMTI010000119         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_471743, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000119 DATMTI010000000
VERSION     DATMTI010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471743"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..518)
                     /locus_tag="VN039_02015"
     CDS             complement(54..518)
                     /locus_tag="VN039_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44792.1"
                     /translation="MRRSPAEVVKRYVLLDQKGARLDAPSFETLTPYIEWKEEPAWGR
                     VVVVEKPTVPEDYRKWEILNNLEVIIPVTFHVLGAVYLKTAIFVPEETTEEVRFHVKV
                     VDGYWRIVSPVIPPHVGLKRIVSFAREAEAHEQDDAQRIVLAALIDSLRKVE"
     gene            928..1356
                     /locus_tag="VN039_02020"
     CDS             928..1356
                     /locus_tag="VN039_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HWV44793.1"
                     /translation="MFTLRWRWIRVIGLLILPCTFSLTFPVSIAAADLGNRPPNVDTI
                     ISPPGQIEPPSSLATPPPKAYDLLKQLEERSGRPLPGYIGGRDFQNRERRLPRGHYRE
                     YDVNPKIRGRGRDAERIVIEQRTGKAYYTGDHYRTFVPLN"
BASE COUNT          362 a          457 c          385 g          326 t
ORIGIN      
        1 gatcagcgta ccgtccaagt cgaaaatcat caaatccacc ggaattttag ccattactca
       61 acctttcgca gagagtcaat gagggcggca agcacgatgc gttgagcgtc atcctgttca
      121 tgggcttccg cctctcgcgc gaagctcact atccgtttca atccgacgtg cggaggaatc
      181 accggcgaaa cgatacgcca gtaaccatcc acaaccttca catgaaaacg aacttcttcc
      241 gtcgtttcct caggcacgaa gatcgcagtt ttcagataga cggccccgag cacgtgaaat
      301 gtgaccggga tgatcacttc cagattgttg aggatctccc actttcgata gtcctccggg
      361 acggttggtt tctcgacgac aacgacacgg ccccatgcag gttcttcttt ccattcaatg
      421 tagggagtca aggtttcgaa tgatggagcg tctaaacggg ctcccttctg gtccaaaagc
      481 acataacgct tcacgacttc cgccggagaa cgtctcatcg aaccgctcgg attcagttgt
      541 gcgttcgaag ggacggccag aacgaggaca gccagagtga ctagataatg gacgaggcgc
      601 ctcggtaaag gattcacgcg tctaatcgga tggatctcac acagcgcatg catagatttc
      661 gcacgatgac cctggacgta cgttcgacac ttgtttattc agatgtacat tcagccgccc
      721 cattctagca aacggaactg aagacatcca ggtaatgagg tggaaccgct ccgcttgaca
      781 gcagtgctct ggtggctctt cagcgacacc tcgccgaaga atgtcctcct acgtaggcgg
      841 gtgatggcgc ctgcgtcgtt tgaccttcgt gaaaagagaa tgctacggtc gtttttcagg
      901 gcaacgtctt gcaaggaaga agagcctgtg ttcacccttc gatggagatg gattcgggtc
      961 atcggtcttc tcatcctgcc gtgcacgttc agcctgacgt tccctgtctc catcgccgct
     1021 gccgacctcg gtaacagacc tccgaatgtc gacaccatta tcagcccgcc gggtcaaatc
     1081 gagccaccgt cctcgctcgc aacacctccc cccaaagcct acgacctgct caaacagctc
     1141 gaagagcgaa gcgggagacc gctgccgggc tacatcggtg ggcgtgactt ccaaaaccga
     1201 gaacgacgtc ttccacgagg ccactaccgg gagtatgatg ttaatccaaa gattcgaggc
     1261 cgcggacgcg acgccgaacg aatcgtcatc gagcagcgaa ccggaaaagc ctactatacc
     1321 ggagaccact accggacctt tgtccctctt aactgacgaa catcgctatg gcaggaaact
     1381 ctaccttaca gactcatctt tgcaatgcca cacctccctg gtcggctctt cttgtcgttc
     1441 ctcgcgggac ctcagcctcg ggggtggtga aaccgccgca cggatatgcc ctgcgtacca
     1501 tccagggaaa gaactgccga actccctcag
//
LOCUS       DATMTI010000120         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_811096, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000120 DATMTI010000000
VERSION     DATMTI010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811096"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VN039_02025"
     CDS             <1..624
                     /locus_tag="VN039_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HWV44794.1"
                     /translation="VETSETGIGGYKNIVALIEGKGAYSHFKYEAGVHRVQRVPVTEA
                     SGRIHTSTVTVAVMPEVDEVDVQIDPKDLRIDTFCSSGAGGQSVNTTYSAVRITHLPT
                     GMVVTCQDERSQLKNRTKAMRTLRARIVEAEREKQEAEIAQNRKSQVGTGERSEKIRT
                     YNFPQNRVTDHRVGMTLHKLELVMEGDLDEIVHALKAQQQQAETAKV"
     gene            627..1517
                     /gene="prmC"
                     /locus_tag="VN039_02030"
     CDS             627..1517
                     /gene="prmC"
                     /locus_tag="VN039_02030"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWV44795.1"
                     /translation="MPAPIITGPKTIGVLITWAQHSLVTAGSTNASQEALWLLAYALG
                     MKHHELASRRDQTVSGEGLVRAESVVSRRVAREPLQYILETQEFCGLDFHVNPAVLIP
                     RPETELLVQEALREGGFVEGGVLVDVGTGSGCVAVTLATILSGMRIFALDCSRDALTV
                     ARGNAERHGVNDKITWKEGDLLEPLRECNLVGAVDAIVSNPPYIADEVWGGLQPEVRD
                     FEPRVALVAGRRGTEFHERLLHEARPFLASGGLLVMELGQGQAPLVRQAAEQVGGYIG
                     LQTVKDEGGIERVMIARRAG"
BASE COUNT          364 a          350 c          489 g          327 t
ORIGIN      
        1 gtcgaaacat ctgagaccgg tattggaggg tataagaaca ttgtcgcatt aatcgaaggc
       61 aagggagcct acagccactt taagtatgaa gccggcgttc accgtgtaca gcgggttccg
      121 gtcaccgaag cgagtggtcg cattcacacg tcgaccgtga cggtggcggt catgccggag
      181 gttgatgaag tggatgtgca gattgatccg aaggatttgc ggatcgatac gttttgctca
      241 tctggggccg gtggacagag tgtaaacacc acctattcag ccgttcgtat tacgcatctg
      301 ccgacgggta tggtcgtgac gtgccaggat gagcgatctc agttgaaaaa tcggaccaaa
      361 gccatgcgga ccttgcgtgc caggattgtc gaggctgaac gggaaaagca agaagcagag
      421 atcgctcaga atagaaagtc tcaggtggga accggagaac gaagcgagaa aattcgaacc
      481 tacaattttc cacagaatcg ggtcacggat caccgagtcg gcatgacgct tcacaagctg
      541 gaattggtga tggaaggtga tcttgacgaa atcgttcacg ccctgaaggc gcaacagcaa
      601 caggctgaga ccgccaaagt ataacgatgc ccgcccccat tattacaggt ccgaagacaa
      661 ttggcgtact gatcacttgg gcccaacact cattggttac agctggatcg acgaatgcgt
      721 ctcaagaagc gctctggtta ttggcctacg cgttgggaat gaagcatcac gagttggcta
      781 gtcggagaga ccaaacggtg tcaggcgaag gactggttcg tgccgaatca gtagtatcgc
      841 gacgggtggc gcgtgaaccg ctacagtaca tcttggaaac acaagagttt tgtggtctcg
      901 actttcatgt gaacccggcg gtgttgattc ctcggccgga gacggaactt ctcgtccaag
      961 aagctcttcg agagggcggg tttgtggaag gtggcgtgct ggtcgatgtg ggaacaggct
     1021 ccggttgtgt ggcggtcacg ttggcgacta ttctgagcgg catgcggatt tttgctctgg
     1081 actgctccag ggatgcgttg actgttgcaa ggggcaatgc ggagagacac ggagtcaatg
     1141 acaagattac ttggaaggaa ggggatcttt tggagccctt gcgcgaatgt aatttggtcg
     1201 gagctgtgga tgcgatcgta tccaaccctc cctacatcgc tgatgaggtc tggggcggtt
     1261 tgcagccgga agtccgggat tttgagccgc gggtggcgtt ggtagctggg cggaggggaa
     1321 cagagtttca tgaacgattg ctccacgaag cacgaccatt cctcgcgtcc ggcggattgc
     1381 tcgtcatgga acttggtcag ggccaagctc cgcttgtgcg gcaagcagcc gaacaagtcg
     1441 gaggctacat aggccttcaa accgtcaaag atgaaggcgg tatcgagcgg gtgatgatcg
     1501 cgcggcgagc aggttaagac caacggcatg
//
LOCUS       DATMTI010000121         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_598837, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000121 DATMTI010000000
VERSION     DATMTI010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598837"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VN039_02035"
     CDS             complement(<1..351)
                     /locus_tag="VN039_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWV44796.1"
                     /translation="MTEQRKDSIILLIHCKDRKGIVARVSGFIHDFGGNILDSDHHTD
                     EETNDFLMRMEFSTEGFQIPSDDIPAAFAPVAKVYQMHYEVYVSSRRTRVALLVSKQD
                     HCLADLLQRHRRDEL"
     gene            complement(413..1873)
                     /gene="rimO"
                     /locus_tag="VN039_02040"
     CDS             complement(413..1873)
                     /gene="rimO"
                     /locus_tag="VN039_02040"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWV44797.1"
                     /translation="MSQPLIIPRRAQSVKTAGVRRTTTTIGFVNLGCSKNQVDSEIML
                     GSLVTDGFQLTGDPKQAEVVIVNTCGFIEEAKEESINTILEHGHLKKTGTCRVLIAAG
                     CLAQRYQGDLLKELPELDAVVGTGEFGKIAEICRDLLAPKKRHQRLWISQPPYLYDEL
                     APRLRLGKQHSAYVKIAEGCNRNCTFCAIPLMRGKQRSRPVESIVAEARQLAAEGVKE
                     INLISQDTVNYGVDLGLREGLVRLLRELVKVEGLRWIRPFYLYPQQVTDDLLALYAGE
                     EKITKYIDMPLQHINDRMLNRMHRLGNRASIEALVDRIHTRIPGVTFRTAFIVGFPGE
                     TDAAFAELRDYVEQTEFDRVAVFHYSDEEGTGAADLDEKVEREVMDERRNALLSIQES
                     ISTAKGRAKIGSVLDVLIDERSAETEGVLEGRHEGLAPEIDGVVYIDEGSTSSFHVCD
                     PRPEMPSPKPGDCCTVEITDAAAYDLVGRIVQNSVC"
     gene            1964..2500
                     /locus_tag="VN039_02045"
     CDS             1964..2500
                     /locus_tag="VN039_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWV44798.1"
                     /translation="MIRTFQGVKPTVPKSCFIEETAVVIGDVVIGEDCSVWFNAVIRG
                     DVNYIRIGNRTNVQDLCMLHVTHDIHPLVIGNDVTIGHHVVLHGCTIHDRVLVGMGAI
                     IMDDVVIGEDSVVGAGALIVEGTIVPPKSLILGAPAKVKRPVTDEELAWIKESAENYV
                     KYARQYMDDSTKKTGFEL"
BASE COUNT          554 a          648 c          721 g          593 t
ORIGIN      
        1 cagttcgtct cggcggtgtc gctgaagcag atcggccaga cagtgatctt gtttcgagac
       61 cagcaaagcg acacgagtgc gccggctgga cacatacact tcatagtgca tctgatatac
      121 tttggcgacc ggagcaaagg cggcaggaat gtcgtcagag ggaatctgga acccttccgt
      181 ggaaaattcc attcgcatga ggaaatcgtt cgtctcttcg tcggtatgat gatcggagtc
      241 gagaatgttc ccgccgaagt catggatgaa tccagacact cgcgcgacga tgcctttacg
      301 gtctttgcaa tggatcagca ggatgatgga atcttttctc tgttccgtca tgggttctct
      361 ctcgtctgat ggcgatcgct atgagtgtag caggagcggc gcgaatccac gctcagcaga
      421 cggaattctg gacgatgcgt ccaacgagat cataagctgc tgcatcggtg atctcgacgg
      481 tgcagcaatc gcccggtttg ggagaaggca tttcggggcg gggatcgcat acgtggaagc
      541 tcgaggtcga gccttcgtcg atgtagacaa caccatctat ttcgggcgcg agcccttcat
      601 ggcgcccttc caggacgccc tccgtttctg ctgatcgctc atcgatgagc acgtcaagga
      661 ccgaaccgat tttggccctc cctttggctg ttgaaatcga ctcttgaata gagaggaggg
      721 cattgcggcg ttcgtccata acttctcgtt cgactttctc gtccaagtcg gccgcccccg
      781 tcccttcctc gtccgagtaa tgaaacacag cgactcggtc gaactccgtc tgttccacgt
      841 agtcgcgcag ctcggcaaaa gctgcgtcgg tttctccggg aaaaccgacg atgaaagcgg
      901 ttcggaaagt cactccggga atgcgggtat ggatacgatc gaccaatgct tcgatcgagg
      961 cgcgattacc gagtcgatgc atgcggttga gcatccggtc gttgatgtgc tgaagcggca
     1021 tgtcgatata tttcgtgatc ttttcttcgc cggcatagag ggcgaggagg tcatctgtga
     1081 cttgctgcgg gtagagataa aacggacgga tccatcggag gccttcgact ttgaccaatt
     1141 cgcgaagtaa acggaccagg ccctcgcgta gaccgagatc cacaccatag ttgaccgtat
     1201 cttgcgagat cagattgatc tctttgacac cttcagcagc gagctgccgc gcttcggcga
     1261 cgatcgactc tacaggccta ctgcgctgct tgccgcgcat cagcggaatg gcgcaaaagg
     1321 tgcaattgcg gttgcatccc tccgcgattt tcacataggc gctatgctgc ttgccgagtc
     1381 tcaggcgagg tgcgagttca tcgtagagat agggtggttg gctgatccag aggcgctgat
     1441 gacgtttttt aggagccaac aagtctcggc agatctccgc aatcttgccg aactccccgg
     1501 tgccgaccac cgcatccaat tccggcaact ctttcagcaa gtctccttgg taccgctggg
     1561 ctaaacaacc ggcggcaatc aagacacggc aggttcctgt tttcttcaga tgtccatgtt
     1621 cgaggatggt gttgatcgat tcctctttgg cctcttcgat gaagccgcag gtattgacga
     1681 tgaccacctc ggcttgtttc gggtcaccgg tcagttgaaa cccatcggtc acgagagacc
     1741 cgagcatgat ttccgagtcg acctgatttt tggagcaacc gagattcaca aatccgatcg
     1801 tagtggttgt acgacggact ccagccgtct taaccgattg ggcgcgcctt ggaatgatca
     1861 atggctgaga cataggggca gtctacggga gggtcaaaaa gtctgtcaat gagacccttg
     1921 tagacccttg cgggaggccg ttcacttcac ctacagtgcc gacatgattc gcacctttca
     1981 gggcgtcaag ccgaccgtcc caaagtcgtg ctttattgaa gagaccgcgg tcgtgatcgg
     2041 cgatgtcgtc ataggtgaag attgcagtgt gtggttcaat gccgtgatcc ggggcgatgt
     2101 gaactacatc cggatcggta accggacaaa tgtccaggac ctctgcatgt tgcatgtgac
     2161 tcatgatatc cacccgctgg tcatcggcaa tgatgtcacg atcggacatc acgtcgtact
     2221 gcatggctgt acgatccatg atcgtgtatt ggtaggaatg ggggcaatca tcatggatga
     2281 cgtagtgatc ggagaggatt cagtagtggg agctggagca cttattgtgg agggcacgat
     2341 cgttcccccg aaaagtctga tcctcggcgc accggccaag gtcaaacgac cggtcacgga
     2401 cgaggagttg gcctggatta aagaatcggc ggagaattat gtgaagtatg cgaggcagta
     2461 tatggacgat tcaacaaaga aaaccggttt tgagctttga gtaatcgcct ttaccc
//
LOCUS       DATMTI010000122         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_47210, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000122 DATMTI010000000
VERSION     DATMTI010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47210"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="ccmC"
                     /locus_tag="VN039_02050"
     CDS             <1..738
                     /gene="ccmC"
                     /locus_tag="VN039_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HWV44799.1"
                     /translation="KYSSPQAFYPLAGHLIPWFACVAVALMGVGLYMGFFVAPTDFQQ
                     GEAYRIIFVHVPAAWMSMFLYVVMAVWAGIGMGLNARLSFMMAQAIAPTGAMFTFLAL
                     LTGAMWGKPTWGAWWVWDARLTSELILLFQYAGVMLLRTSIDDVRRADRASAVFALVG
                     VVNVPIIYFSVQWWNTLHQGASVSMATGSKMASTMLTAMLLMTLAFWLYSIAVILARV
                     RCVMIERESLPVWNEKPAVIQEAVEAR"
     gene            738..923
                     /gene="ccmD"
                     /locus_tag="VN039_02055"
     CDS             738..923
                     /gene="ccmD"
                     /locus_tag="VN039_02055"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HWV44800.1"
                     /translation="MQWGSASEFFAMGGYGLYVWTSFVATALCMVWEVLALWRRRAAA
                     RAEQRAALLGGIDETTA"
     gene            907..1398
                     /gene="ccmE"
                     /locus_tag="VN039_02060"
     CDS             907..1398
                     /gene="ccmE"
                     /locus_tag="VN039_02060"
                     /inference="COORDINATES: protein motif:HMM:NF009729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HWV44801.1"
                     /translation="MKPRHKRFAFIGLGLLVLGVATVLILNAFQSNLVFFFTPTQVVS
                     GEVPQGRSFRIGGMVEDGSLVRENDGLTVRFIVTDTAKRVPVTYKGILPDLFKEGKGA
                     VAQGKLNADGTFVASEVLAKHDENYMPPEAAEALAKAKASGAQQSKSLVVPQGRQGRE
                     DPL"
     gene            1395..>3078
                     /locus_tag="VN039_02065"
     CDS             1395..>3078
                     /locus_tag="VN039_02065"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HWV44802.1"
                     /translation="MIPEIGHFALILALCVAVVQGILPIYGAAVGNSALMAVAKPAAR
                     GQFLLVLTAFCCLGYAFAEKDFSVLYVAATSNSQLPLHYRLAAIWGAHEGSLLLWTFI
                     LTLWMFAVTLFSAHLPEATRSRILGVMGLVSVGFLLFMLTVSNPFERLIPAALDGRDL
                     NPLLQDPGMVIHPPMLYMGYVGFSVAFAFAIAALLGGNLDAAWARWSRPWTTVAWCFL
                     TVGIAMGSGWAYYELGWGGWWFWDPVENASFMPWLAGTALVHSLAVTDKRGGFKVWTV
                     LLAIMAFSLSLLGTFLVRSGVLTSVHAFATDPKRGLFILVFLAVVIGGSLALYAWRAP
                     RVGLGGSFAMVSREGMLLANNVLLVAAMGSVLLGTLYPLFLDALDLGKISVGPPYFDS
                     VFVPLMVPALFLMGVGPLTQWKKAELPDLAKRLRWAFGVSVVTALILPFVLGTWTPLL
                     SLGLLLAVWIVTTSVIGLRERLTHVGGHGLAGRLANVPRSYWGMLVAHCGIAVFIVGV
                     TMVKGFETEKDVRMNVGETASIGDYTFRFDGAQDVVGPNYTAARGIFRVSLDG"
BASE COUNT          501 a          811 c          969 g          797 t
ORIGIN      
        1 aagtattcct ctccccaagc cttttatcct ctggcgggtc acctcattcc atggttcgct
       61 tgtgtggcgg tcgcgctcat gggggtgggg ctctatatgg ggttcttcgt cgcgccgacc
      121 gactttcagc agggcgaagc gtaccggatt atcttcgtcc atgttcccgc ggcctggatg
      181 tcgatgttcc tctatgtcgt catggctgtg tgggccggca tcgggatggg actcaatgcg
      241 cgtctttcct tcatgatggc gcaagcgatc gctcctaccg gggcgatgtt tacgttcttg
      301 gcattgttaa ccggggcgat gtggggaaag cccacatggg gagcctggtg ggtgtgggat
      361 gccaggctga catcggaact catcttattg ttccaatatg cgggggtcat gcttttgcgg
      421 acctcgatcg atgatgtgcg ccgcgcggac cgggcgagcg ccgtctttgc cctcgttggg
      481 gtggtcaacg tcccgatcat ctacttttcc gtgcaatggt ggaatacctt gcatcaaggg
      541 gcgtcggtca gcatggcgac gggatcaaaa atggcgtcaa ccatgctcac ggccatgttg
      601 ctgatgacat tggcgttctg gctgtacagc atcgccgtca ttctcgccag ggtacgctgt
      661 gtcatgattg aacgggaatc cttgccggta tggaatgaga agccggccgt cattcaggaa
      721 gcggtggaag ctcgctgatg cagtggggaa gcgcatcgga attttttgcg atgggaggtt
      781 acgggctcta tgtgtggacc tcatttgtgg ctacggctct gtgcatggta tgggaagtgt
      841 tggccttgtg gcgtcgacga gctgcggcgc gcgccgaaca gcgcgctgcg ttgttaggag
      901 gcatcgatga aaccacggca taaacgcttt gcctttatcg gcctggggct gctcgtcttg
      961 ggtgtggcga cggtgctgat cctgaacgcc tttcagagta atctcgtgtt cttctttacg
     1021 cccactcaag tggtaagcgg cgaggtgccg caaggacgca gtttccgcat cggcggcatg
     1081 gttgaagacg ggagtctcgt ccgtgaaaac gacgggttga cggtccgttt catcgtcacc
     1141 gataccgcga agcgcgtgcc ggtgacctat aaaggaattc tgcccgacct ctttaaggaa
     1201 gggaagggtg ccgtggcaca aggaaaactc aacgccgacg gcacgtttgt cgccagcgaa
     1261 gtcttggcga aacatgatga gaattacatg ccgcctgaag cggccgaagc cttggcaaaa
     1321 gccaaggcct ccggggccca gcaaagtaaa tcactcgttg tccctcaagg taggcagggt
     1381 agggaagacc cactatgatt ccggagatcg gtcattttgc gttgattctt gcactgtgcg
     1441 tcgccgtggt gcagggcatt ctccccatct acggagccgc ggtcgggaac tcggcgctga
     1501 tggccgttgc caagccggcg gcgcgcgggc aatttctctt ggtcctcacg gcgttttgct
     1561 gcctcggcta tgcgtttgcc gagaaggatt tctctgtgct gtatgtcgca gccacgtcga
     1621 actcccagct tcccttgcat tatcggctgg cagcgatttg gggcgctcac gaaggatcgc
     1681 tcctcttgtg gacctttatt ttgaccctat ggatgttcgc cgtcacgctt ttctccgctc
     1741 atctccccga agccacacgt tcccgcattc tcggcgtgat gggtcttgtg agtgtcggct
     1801 ttcttctgtt catgctgacc gtgtcgaatc cgttcgaacg gctgattccc gccgcgcttg
     1861 acgggcgtga tctcaatccg ctcttgcaag acccgggtat ggtgatccat ccgccgatgc
     1921 tctatatggg ctatgtcggt ttttcggtgg ccttcgcgtt tgccatcgcc gcgttattgg
     1981 ggggcaacct cgatgcggcc tgggcccgct ggtctcggcc atggaccacc gtcgcctggt
     2041 gttttttgac ggtcggcatt gcgatgggaa gcggctgggc ctattacgaa ttgggatggg
     2101 gtgggtggtg gttctgggac ccggttgaga atgcttcatt catgccgtgg ttggccggaa
     2161 cggcgttggt tcattcactg gccgtgaccg acaagcgggg agggttcaaa gtatggaccg
     2221 tcctgctcgc catcatggcg ttctctttga gtctccttgg aacatttctt gtccgctccg
     2281 gcgtgttgac ctccgtgcat gcctttgcca ccgatccaaa acgtggtctg ttcattctgg
     2341 tctttttggc cgtcgtcatc ggaggatcgc tcgccttgta tgcctggagg gcgccgcgcg
     2401 tcgggttggg cggcagtttc gccatggttt cgcgcgaagg gatgctgctg gcgaacaacg
     2461 tgttgctggt tgccgcgatg ggctcagtgt tgctgggtac gttatacccc ttatttttgg
     2521 atgcgctgga tctcgggaag atttctgtcg ggcctccata cttcgattcc gtcttcgttc
     2581 cattaatggt gccggctctc ttcttaatgg gagttggtcc gctgactcag tggaagaaag
     2641 ccgaattgcc cgatctggcc aagcggttac ggtgggcttt cggcgtcagt gtggtaacgg
     2701 cgcttatctt gccatttgtc ctgggtacct ggactccctt actcagcctc ggtctcttat
     2761 tggcggtctg gattgtgacg acgtccgtca tcggtttgcg cgaacgactc acccatgtcg
     2821 gcggccatgg tctcgcaggt cgtttagcca acgtgccacg atcttactgg ggcatgctcg
     2881 tggcacactg cggcatcgcg gtgtttattg tcggagtgac gatggtgaaa gggttcgaaa
     2941 ctgagaaaga tgtgcggatg aacgtcggag agacggcgtc gatcggcgac tatacgttcc
     3001 gcttcgatgg cgctcaagat gtggttgggc ccaactatac agctgcgcgc ggcatctttc
     3061 gtgtgagcct ggacggtc
//
LOCUS       DATMTI010000123         4478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_683605, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000123 DATMTI010000000
VERSION     DATMTI010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683605"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VN039_02070"
     CDS             <1..296
                     /locus_tag="VN039_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWV44803.1"
                     /translation="SRAVLDLVSKGEAELGIVYQTDALTAKRVRIIDTAPADSHRPVT
                     YGIAAPWTAQNIAGARELVSFLMSAPVQTELKKYGFERASSDVSFTQRQEVKP"
     gene            293..991
                     /locus_tag="VN039_02075"
     CDS             293..991
                     /locus_tag="VN039_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44804.1"
                     /translation="MNGGNITDNQSVARSVRAVLILSLGLMIGCAGTLVGPQTTIYRS
                     GLNHVYLETDPDSSSNDHPTQLTSSDVGSLLRGVRVWKQRNFLHRLYSGEPERTRAFR
                     NEEVAELAPVLSKALEIASADQRVSFHLSHVTEHGQEETTTGWLSIRGNLLYLDLSEV
                     HDRHGPGPDISKYDREMPNVPEQAPAFHATFEPEEYLSKVRSRWRLFMPDQREELQIR
                     YRDALEALTKISRS"
     gene            1367..2200
                     /locus_tag="VN039_02080"
     CDS             1367..2200
                     /locus_tag="VN039_02080"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HWV44805.1"
                     /translation="MMTDRLIKRAFHVGIVASTSEGAALCYRTLCREADEVTGHNMHP
                     EITLHALPLRLYLDAIDLDDWASVAGLMSYSAAKLVRAGADVIVCPNNTLHKAFDFVE
                     SPVPWLHIAKPVVKEITQRGWRRVGILGTQIVMEGSIYSHRLEQLGINVMIPRKDDRV
                     RIQHIIRAELIAGLFTAQSRRFLQKVIAEMAADGAEAVILGCTELPLLLSDEQAALPL
                     LNSTSLLARAALYHSRERPPSLGIPKEASEACTLGQTTTALMDANVYRSWAERRERSM
                     L"
     gene            2197..3231
                     /locus_tag="VN039_02085"
     CDS             2197..3231
                     /locus_tag="VN039_02085"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWV44806.1"
                     /translation="MRIKQSASVSITVFLTGFFMWVLSIHAAEPVVILNVSYDPTREL
                     YQEFNAAFARHWLAKTGQKITIKQSHGGSSKQARAVIDGLEADVVTLALAYDIDAISE
                     KAALIPSNWQARFPQNSAPYTSTIAFLVRKGNPKNIKDWDDLVKPEVSVITPNPKTSG
                     VARWNYLAAWGYALRRSGGDDGKAKEFIAQLFKNVPVLDSGARGATTTFVERGIGDVL
                     INWENEILLGGEGLGKDKFDIVVPPTSILAEPTVSLVDKVVDKRGTRTVAQAYLEYLY
                     SEEGQEIAAKHYYRPRLASVAAKHAKFPKLELFTLQEFFGDWQKANKAHFADGGTFDQ
                     VYLQLSKERR"
     gene            3323..4159
                     /gene="cysT"
                     /locus_tag="VN039_02090"
     CDS             3323..4159
                     /gene="cysT"
                     /locus_tag="VN039_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HWV44807.1"
                     /translation="MRLVLKQPTVLPGFGLTLGFSLFYLSLIVLIPLSGLFVKTSTLS
                     GEQFWTLVTDPRAIASYQLSFGASLLGALINGLFGSIIAWVLVRYRFPGRSLIDALVD
                     LPFALPTAVAGITLTAIYASNGWIGRYLEPFGIKVAFTPLGVLVALTFIGLPFVVRTV
                     QPVLQDLDKEVEEAATTLGANRWQTFRAVIVPELWPALLTGIAMAFARAVGEYGSVIF
                     IAGNMPLKSEITPLLIMTKLEQYDYAGATALGVVMLVASFVLALAINLLQWWSSARHM
                     NQ"
     gene            4166..>4478
                     /locus_tag="VN039_02095"
     CDS             4166..>4478
                     /locus_tag="VN039_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44808.1"
                     /translation="MNSITMAADKDLQWKESLTTENSLVRRLLIAAAFLYLTLFLFIP
                     IAAVFAEALKGGIAAYVESLLTEDALASIRLTLLVAAVAVPLNALFGVAAAWAIAKFD
                     FA"
BASE COUNT          981 a         1163 c         1282 g         1052 t
ORIGIN      
        1 attcccgggc ggtacttgat ctcgtttcaa agggagaagc ggaactcgga atcgtctatc
       61 aaaccgatgc gctcacggca aaacgtgtgc gcatcatcga tacggctcct gccgacagcc
      121 ataggccggt cacctacgga attgcggctc catggaccgc acaaaacatt gcgggggcgc
      181 gggagttggt ctccttcttg atgtccgccc ccgtccaaac agaactgaag aaatatggat
      241 ttgagcgagc aagctccgat gtcagcttca cgcagcggca ggaggtcaag ccatgaacgg
      301 agggaacatc accgacaatc agtcggtcgc acggtctgtc cgtgcagtgc ttattctaag
      361 ccttggtttg atgatcggtt gcgccggtac tctggttggg ccgcaaacga cgatttatag
      421 gtcgggatta aaccatgtgt accttgagac agaccctgat tcgagctcaa acgatcatcc
      481 gacgcaattg acttcgagcg atgtcggttc gttgctacga ggggttcgtg tttggaaaca
      541 gcgcaatttt ctccaccggc tctattccgg cgagcccgaa cggacgaggg catttcgtaa
      601 tgaggaggtg gccgagcttg caccggtgct ctcaaaagcc ttggaaatcg cctcagctga
      661 tcagagagtg tctttccacc tgagtcatgt gactgagcac ggacaagagg aaaccacgac
      721 tggttggctg tccattcggg gaaatctgct ctatctcgac ttgagcgagg ttcatgatcg
      781 gcatggtccc gggccggaca tcagcaagta cgaccgtgag atgcccaacg tgccggaaca
      841 ggcccccgcc tttcatgcga cgtttgagcc ggaagagtat ctgtccaagg tccgttctag
      901 gtggcgactg ttcatgcccg atcaacggga agaactgcag attcggtacc gagacgcgct
      961 ggaggcactg acaaaaattt cccgatcgta agcgaacatt ccggtgcaac cggccatgag
     1021 cagattgtcg tcgccggtta tcgaatcttg aggtgatggt atggccattc tgattgtagg
     1081 cggtgactcg gcggaaatat tccggcggtg gggctgccga atcgcggata cacgtggaac
     1141 attggacggg gcgcaagccg cgtgatttgg tacggtcgat tccaaaagcc actgatgcgg
     1201 tcgtcgtggt gctggaccgg gtgaatcatg cgttggcgaa aagggtgggg agagaagcct
     1261 cgcgacgtga tcttcctgtc tattttcgaa aacgagtccg tcggagcgat gcaggcactc
     1321 agcctatacc agactcgctg tatccattcc ggtttcgaga gaactcatga tgacggatcg
     1381 cttgatcaag agggcgttcc acgtcgggat cgtggccagc acgtccgaag gcgcggcact
     1441 gtgctatcga accttgtgtc gtgaggcaga tgaagtcaca gggcacaaca tgcaccccga
     1501 aatcactttg cacgcgctcc cacttcgctt gtatctggac gcaatcgatc tggatgattg
     1561 ggcaagtgtc gccggcctga tgtcttattc ggcagccaag ctggtacgag caggcgctga
     1621 cgtcatcgtt tgtccgaata atacgctgca caaagcgttc gatttcgttg agtcgccggt
     1681 tccatggctg catatcgcca agccggttgt gaaagaaatt acacagcgcg gatggcgccg
     1741 agtcggaatt ctcggaacac agattgtcat ggaaggatcg atctattctc ataggttgga
     1801 gcagttgggc atcaatgtca tgatcccgag gaaggacgac agagttcgaa ttcaacacat
     1861 tatcagagcc gaattaatcg cgggactgtt tacagcccaa tctcggcggt ttctacagaa
     1921 agtgattgca gagatggcgg cagatggagc ggaggcggtg attctcggct gcaccgaact
     1981 acctctgctt ctctccgatg aacaggcggc tcttccgcta ctgaactcga cgagcctgtt
     2041 ggcaagggcc gcgctgtacc actctcggga gcgaccgccg agtttgggaa ttccaaagga
     2101 agccagcgaa gcatgtactc tcggacagac aacaaccgcg ttgatggatg ctaacgtcta
     2161 tcggtcatgg gcagagagaa gagaaaggag catgttgtga ggataaaaca gtcggcttcc
     2221 gtatccataa cggtattttt gaccggcttc ttcatgtggg ttttgtcgat ccatgctgct
     2281 gagccggtgg tcattctcaa tgtgtcatat gatccgacgc gggaactgta tcaggaattc
     2341 aatgcggcgt ttgccagaca ctggctggct aagaccggcc aaaagatcac gattaagcaa
     2401 tctcatggag ggtcgagcaa gcaggctcgg gccgtcatcg atggcctgga agccgacgtg
     2461 gtgacattgg ccttggcgta cgatattgat gccatttccg aaaaggcggc gctcattcca
     2521 tccaactggc aggcgcggtt ccctcaaaac agcgcgccat atacctcaac gatcgccttt
     2581 ctcgttcgca aaggcaaccc taaaaatatt aaggattggg atgatctggt taagccggag
     2641 gtttcggtca tcacccccaa cccaaagaca tcgggggttg cccggtggaa ttacttggct
     2701 gcctggggct atgcgctgcg acgatccggg ggcgatgacg gcaaagccaa agagtttatc
     2761 gcccagttgt tcaagaatgt gccggttctc gactcaggag cacgtggcgc gacgacgacg
     2821 tttgtggagc gcggtatcgg cgatgtcctg atcaattggg aaaacgaaat tttgttgggc
     2881 ggtgaaggcc tcggaaaaga caagttcgac attgtggtgc ctccgaccag cattctggcg
     2941 gagccgacgg tcagtttggt ggacaaggtt gtagacaaga gaggtactcg gaccgtcgct
     3001 caagcgtatc tcgagtatct gtattccgaa gaaggtcagg agatcgccgc caagcattat
     3061 tatcgtcctc gtcttgcatc ggttgccgcg aagcacgcga agtttccaaa gttggagtta
     3121 ttcacgctgc aggagttttt cggggattgg cagaaagcca ataaggccca cttcgccgac
     3181 ggagggacct ttgatcaagt ctatttgcag ctcagcaagg aacgccgatg aggtcgatac
     3241 aaagccgatg gattgcggca atccctcgtt tccgtcgacc actatagcga tgtcgtttac
     3301 ggtttaattg ggagaaaccc cgatgcgtct agtgttaaaa cagccaaccg ttcttcctgg
     3361 attcggccta actttggggt tttctctttt ttatctcagt ctcatcgtgc tgattcctct
     3421 cagtggactc tttgtgaaga ccagcacatt gtctggcgaa cagttttgga ccctcgtcac
     3481 cgatccgcga gcgattgcct cttatcaact gtcattcggt gcctcgctgc tcggagccct
     3541 cattaacgga ttgttcggat cgatcatcgc gtgggtcttg gtccgatacc gctttccagg
     3601 gcggtcgctt attgatgcgc tcgtagacct gcctttcgct ctgccgactg cagtcgccgg
     3661 cattacgttg acggcgatct atgcatcgaa cggatggatc gggcgttatc tcgaaccgtt
     3721 cggcatcaag gtggccttca ctccgctggg tgtccttgtc gcattgacct tcattggatt
     3781 gccgttcgtc gtccgcaccg tgcagccggt gttgcaagat ttggacaagg aagttgaaga
     3841 agccgccacc acgctggggg caaaccgatg gcaaacattt cgcgccgtta tcgtacccga
     3901 actctggccg gcacttctga caggaatcgc catggcattc gccagagccg tcggagaata
     3961 tggatccgtc atttttatcg caggaaacat gccgctcaaa tccgagatca ctccattgct
     4021 gattatgacc aagttggagc agtacgacta tgccggcgcc actgcgctgg gcgtcgtcat
     4081 gcttgtggca tcgttcgtct tggctttggc tatcaatctc cttcagtggt ggagcagcgc
     4141 tcgccacatg aatcaatagg aatctatgaa ctccatcact atggccgccg ataaggatct
     4201 gcagtggaaa gaatcgctca cgaccgaaaa ctcgcttgtc cgccggctgc tgattgccgc
     4261 cgcttttcta tacttaacac tcttcctctt cattccaatt gccgcagtct ttgctgaagc
     4321 gttgaaaggc ggtatcgccg cctatgtcga gtcgcttctc actgaggatg cgctagcgtc
     4381 tatccggttg acgcttctgg tagccgcggt ggcggtaccg ctgaacgcct tgttcggtgt
     4441 cgccgctgcc tgggccatcg cgaaattcga tttcgcgg
//
LOCUS       DATMTI010000124         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_5024, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000124 DATMTI010000000
VERSION     DATMTI010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5024"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1869)
                     /locus_tag="VN039_02100"
     CDS             complement(<1..1869)
                     /locus_tag="VN039_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWV44809.1"
                     /translation="MRNVLKHDEEARRFCALYDITESGNWEHTSIPNRLRPLEDIARQ
                     LNMTTDELMELMSRAKPLLYEARRHRIPPGLDDKVITAWNGMMLSAMAEAARVFGNAA
                     YLKSAQRTADFLLRSHAKPDGRLLRTSRGDRAHLDGYLEDYAYLTEGLLDLYEAGSAE
                     SYLQAAARLAEYLISDFLDREQGGFFTTAKHHESLILRHREGMDGAVPSANAVAASAL
                     ARLSFHFDREDWRRAAVAATRAYGRQISRYPRAFAKSLAVIDFLTEGPVELAFVGDES
                     QDNVRALREAVARCYLPNRIVATGSPGHPSSLPLLRDRPAVSGQPTLYICRNYTCRQP
                     ITDPRSVEEALRADQCASTDRAAEPRLLQGTSLSGSATVLDTAAYASRVMARDGEAGG
                     QGFTVLGATGLTTTRLGFGTYRVDTQNPEYREALKKALRGSCNLIDTSTNYTDGDSER
                     LVGSVLAELVASGEIRRDEIIVVSKIGYIQGQNLKLAEAKEQSGRPYPEPVKYGDGIW
                     HCIHPEFLADQLTLSLDRLGLTTLDICLLHNPEYFFSDAAHRGNTDLEKLRTDFYARL
                     ERAFVYFETQVSAGRLRYYGVSSNTVISAADDQEATSLARMVQAAEAAARSAGASTH"
BASE COUNT          390 a          531 c          569 g          402 t
ORIGIN      
        1 gtgggtagag gcgccggcgg atcgggccgc agcttcagcg gcttgcacca tccgcgcgag
       61 cgaggtcgct tcctggtcat cggcagcaga gatgacggta ttggatgaca cgccgtaata
      121 tcgaagtcgt cccgccgaaa cctgggtttc aaaatagaca aaagcacgtt cgaggcgagc
      181 atagaaatct gtgcgaagtt tttccaggtc tgtattgcca cggtgcgccg cgtccgaaaa
      241 gaaatattcg ggattatgca gaaggcagat atcgagcgtt gtcagtccca ggcgatctaa
      301 ggacagagtc agttgatcgg ccaagaactc cgggtgtatg cagtgccaaa tgccgtctcc
      361 gtatttcacc ggttcagggt atgggcgacc ggattgttct ttggcttcgg ccagcttgag
      421 attctgtccc tggatatacc cgattttgga gaccacaatg atttcgtcgc ggcgaatctc
      481 acccgatgca acaagctcgg ccagcacgga tcctaccaat cgttcgctgt cgccatccgt
      541 gtaattcgtc gaggtgtcga tgagattgca agatcctcgc aacgcctttt tcagcgcttc
      601 gcggtactca ggattctgcg tatcgacgcg ataggtgccg aaccctaacc tggtcgtagt
      661 caagcccgtc gcaccgagca ccgtaaaacc ctgtccgcct gcctcgccgt cccgtgccat
      721 tactcgggac gcataggccg ctgtatccag gaccgttgcg cttcccgaga ggctggtgcc
      781 ttgcagcagt cttggttcgg ccgcacggtc cgttgatgca cactggtcgg cccgtaacgc
      841 ctcttcgacg gaacgaggat cggtgatcgg ctgccggcaa gtgtaattcc gacagatata
      901 cagagtcggc tgaccggaaa cggccggccg gtctcgtaag agcggaagag aagatgggtg
      961 ccccggagaa ccggtcgcca cgatgcggtt gggaaggtag caacgcgcca ccgcttcacg
     1021 gagggcacga acattatcct gcgactcatc acccacgaag gccaattcca ccggtccttc
     1081 ggtgagaaag tcgatcacgg ccagactctt ggcgaatgcg cgaggataac ggcttatttg
     1141 tcgaccgtag gcgcgtgtgg ctgcgaccgc ggcgcggcgc caatcttctc gatcaaagtg
     1201 gaaagatagt cgagccagcg ctgaagcggc gacggcgttg gcgctgggaa cggctccatc
     1261 catgccttcc cggtgtcgga gaatgaggga ctcatggtgt ttcgccgttg tgaaaaaccc
     1321 gccctgttcc cggtccaaaa agtcactgat caggtattcc gccagtcgcg cggccgcctg
     1381 gaggtacgat tcagcggaac cggcttcgta gagatccagc aatccctctg tgaggtacgc
     1441 atagtcttca agataaccgt cgagatgggc acggtcgccg cgcgatgtgc gcagtaaccg
     1501 gccgtccggc ttggcatgac tgcggagcag aaagtcggcg gtccgttgag cgctctttag
     1561 ataggcggcg tttccgaaga ctcgggctgc ctcggccatg gccgacagca tcatgccgtt
     1621 ccatgcggtg atcaccttgt catccagtcc gggaggaatg cgatgtcggc gtgcttcata
     1681 caataggggc ttcgcacggg acatgagttc catcaactcg tccgtcgtca tattcagctg
     1741 cctggctatg tcttcgagag gtcgcagtcg attggggatg ctcgtatgtt cccaattgcc
     1801 tgattctgtg atgtcgtaaa gggcgcagaa acgtcgcgcc tcctcatcat gcttgagaac
     1861 gtttcgcact tcagcggggg tccaaacaaa ga
//
LOCUS       DATMTI010000125         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_556884, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000125 DATMTI010000000
VERSION     DATMTI010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556884"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VN039_02105"
     CDS             complement(<1..848)
                     /locus_tag="VN039_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV44810.1"
                     /translation="MPRTEDLTTVQKRFLDGVGSLIAPDKLPDPAPSVDAADLTLSAV
                     AGLYTVASRFHRIEFIFDRLQEARGGVHAELQVNAGWKDDLLGITDISLKSESSREKI
                     AKALKAHDQDTPWRPLLEQGCAKVLRHHRTGDPLVTISSETPIEPLTFALNPLVFDRK
                     VSVLYSNGGQGKSTLALFLAMLSASGSSEAGFSALKGNSLYLDFEDDLSVHARRLHAI
                     QNGHPALRSARVAYRRCVEPLHKFAPALLQQIHQEQIRFVVVDSLLAATGGDASAEAT
                     TQLFIAL"
     gene            complement(835..1191)
                     /locus_tag="VN039_02110"
     CDS             complement(835..1191)
                     /locus_tag="VN039_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44811.1"
                     /translation="MALQWRQPSAAVEAAQILVQIHQALRLSYASPLFFYEPETNPQK
                     RKAAAQQREAQRQQRGQEVRNQGCLLDACKQAEHFLQSRQPVDISAWTDERLNRELNL
                     IADAFRVLEADPYAAY"
     gene            complement(1116..1775)
                     /locus_tag="VN039_02115"
     CDS             complement(1116..1775)
                     /locus_tag="VN039_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HWV44812.1"
                     /translation="MIYERRSRLRGKQQGRLIDLFVAGATARAAAEIAGVNRNTARTF
                     YHRLRQLIASKLPSYDLSGEVEADESYFGGMRKGQRGRAAAGKVPVFGLLKRGGKVFT
                     AIIPNARSRTLLPIIREQVSPDSIVYTDSFTAYDVLDVSEFHHRRVNHSKIFVTKRGH
                     HINGIENFWNQAKRHLRRFNGITPDNFYWFLKECEWRFNGGNHQQLLKQLKSWYKSTK
                     H"
BASE COUNT          392 a          518 c          506 g          402 t
ORIGIN      
        1 agggcaataa agagctgcgt ggttgcttcg gcgctggcat cgccacccgt ggccgccaac
       61 agagagtcca ccacgacgaa ccggatctgt tcctgatgaa tttgttgcaa taacgcaggg
      121 gcgaacttat ggagcggttc cacacagcgg cgataggcca cgcgcgccga gcgaagggca
      181 ggatggccgt tctgaatcgc gtgtaatcga cgggcatgga ccgaaaggtc atcctcgaag
      241 tccaagtaca agctattgcc cttcagggct gaaaatccgg cctccgagct gccggaggcg
      301 ctcagcatgg ccaagaacaa cgcaagggtt gatttgccct ggccgccgtt cgagtagagg
      361 acggacacct tgcggtcgaa cacgagagga ttgagcgcaa aggtgagcgg ttcaatgggg
      421 gtctcactgg aaatggtgac caacggatct ccggtccgat gatggcgcaa taccttggcg
      481 cagccttgtt cgaggagcgg cctccacggc gtatcttggt catgcgcttt gagggctttg
      541 gcaatcttct cgcgtgagct ctccgatttg agcgaaatgt ctgtgatccc cagcagatca
      601 tccttccatc ccgcgttcac ctgcagttct gcatggacac caccccgggc ttcctggagc
      661 cgatcaaaga tgaactcgat ccgatgaaat ctggacgcca cggtgtacag ccccgcgacc
      721 gctgaaaggg tcaggtcagc cgcatcaaca gatggagcag ggtctggcaa cttatcaggt
      781 gcgattaagg aaccgacgcc atccagaaat cgcttctgca ctgttgtaag gtcttcagta
      841 cgcggcatag gggtcggcct ccaatacgcg gaacgcatcg gcgatcaaat tgagttcacg
      901 gttgagccgt tcgtcggtcc atgcactgat atcgaccggc tgtcgggact gaaggaagtg
      961 ctcagcctgt ttacaggcat ccaggaggca tccctgattg cggacttctt gcccgcgctg
     1021 ttgacgctga gcctcacgct gttgtgcggc agctttgcgt ttctggggat ttgtctcggg
     1081 ttcgtaaaag aacagggggc tggcatagct aagccttaat gcttggtgga tttgtaccag
     1141 gatttgagct gcttcaacag ctgctgatgg ttgccgccat tgaagcgcca ctcgcattcc
     1201 ttcaggaacc agtaaaaatt gtctggtgta atgccgttga agcgacgcaa atgccgcttc
     1261 gcttggttcc aaaagttctc aatcccattg atatgatgtc ccctcttagt gacaaagatc
     1321 ttgctgtggt tgacacgtcg gtggtgaaac tccgagacat ccaacacatc atacgccgtg
     1381 aagctatccg tatagacaat gctgtcaggg gacacctgct cgcgaatgat tggcagcagc
     1441 gttctcgacc gggcattcgg aataatcgcg gtaaacacct tcccaccccg ttttaacaag
     1501 ccaaagaccg gcactttccc cgccgctgca cggccccgct ggcctttgcg catcccacca
     1561 aagtagctct catccgcctc gacttcgcca gatagatcat agctaggcaa cttgctggcg
     1621 atgagctgac ggagccgatg gtaaaacgtt cgcgccgtgt tccggttcac accggcaatc
     1681 tcggccgcag cccgcgcagt tgctcctgcc acaaacaggt cgatgagtcg cccttgctgt
     1741 tttcccctta accgacttcg ccgctcgtag atcattcgct aaagctaccc ctctcaggtc
     1801 gctttagcta tgctagcc
//
LOCUS       DATMTI010000126         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_641633, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000126 DATMTI010000000
VERSION     DATMTI010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641633"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..>4405)
                     /locus_tag="VN039_02120"
     CDS             complement(282..>4405)
                     /locus_tag="VN039_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HWV44813.1"
                     /translation="NIKFATENLTKFDYNVKDFEDWYSGFTASADAWALSGSERYLAL
                     LRVLAQEILTRVMRECREAKSLPRNSEDMTEEWLVGELRKRFSFNDTAHKRLQAFLAR
                     RKNKDETLEGYLIEKRELYLKYVELMKRDEHALTEDGQMFLEAAIKGLPKELHATIKL
                     RHPKEGRKFVELVKSARDLQGSEIASQQQEKPQPQARKPQTGTNEPRRCYNCGEIGHL
                     RPQCPKNKEVRRSERFKAKESGGADKKPIAQVDPDDGLDTPIRVSVEMGVNSSGRVPV
                     VAYVDTGASSTVISAVLAERLCARKDWEESKIGAKLADGRATEMLGWLRLVVNFPNGG
                     VASVRAMVLEKLVNGEQCLLGRDALKQVKAQVDWDNGALVLPVVDCDEVVASTEMVDV
                     DVPKEVVEIPVQITKVLEKFKDVFDAPIKECGVVTKHYVTLTTEEPVVDGLRRTSPKQ
                     DEIIEASLKDMLDQGVIEKSTSEYASPVVLARKKDSSWRFCVDYRKLNAITRKDPYPL
                     PRIDEILGSLGAARVMSKMDLQSGFWQIPLANKDRHKTAFITKQGKYQFKRMPFGLTG
                     APTTFQRVMNECLEGIIGEFAFVYLDDIIVYSKTMEEHGKHLAEVLSRLRDKGFQVKR
                     KKCIFAVSELPFLGHVVGNGKLGLDMEKVAAVKKLAPPRNGKELQRFLGSVGYFRRFI
                     ESYARLAKPLTDLTKKDEPYAWGKEQQSAYEELLGRLTSPPILRLPDPTKSFVVRTDC
                     SDYASGGVLLQEHLGELLPVAYVSKKLTKAERNYTTTEKEALAVVRAVKEWWFYLHGN
                     KFVVETDHSALRSVLKARETSGRVARWVMALQELEFEVQHRPGKMMELPDMLSRQEQI
                     LAIDADLELGEAQRQDATLGPIMRALENGDEPKDPDALKLYRQVATYLVIDDGKLFYF
                     RGKKDKSKRLVVPNSLRRDVLLEHHDSLVGAHFGIEKTYANIQERYWWPNQYAETKHY
                     VETCEACARAKKPKVSNEGKWEATVVGDRWQRVSADFLGPVDKTERGNRYLLVFTDYF
                     TRYVVAVPTVDCTAETVAREFVDKIVTKFGAPRELFTDNGPAFASEVVNAICKMAGTH
                     KLFTTAYRPQANGMVERWNGTVAQSLRACMDGDKSSWDWKVPFITFAYNNSKHAATKM
                     APFELLFGRLARLPLDVVLGARAPEERAVAEFTNELLIDLQEGFRVAREASDEMKRAQ
                     ERRATFSGGKAPAYKQGEKVWLDRRDADSTKLDPKFDGPYVVGDVHGRNYVTISKPGG
                     AREKVHVERLKPYKGREVGEVELEEEEPGNDQEVEVDPDPMMLPNDLVGKRIITWWPA
                     EGKWFKGVVSGREKRRHVVKYDDGEVRLERLLGYGKTGVKWKLLERRRSDDRP"
BASE COUNT          960 a         1384 c          998 g         1063 t
ORIGIN      
        1 aaggttctcg gtagcgaact tgatgttgtt cttgctcatt tggttaggaa agacactaac
       61 cggtgaggtc caccaatttg taacgttaga ctacgctgag tagcaaacgc agttgtattg
      121 tttaacacaa tagggaactg agtacacgta actcataaca cgcatatata cttcgcgtag
      181 ttgaccaacg ggagatttca gggaaatctc ccgaataagt ggcaggcgcg ttcacgaacg
      241 atcgccattc actgatcgtc gccacgtcac acttccctcc tttaagggcg gtcgtcgctc
      301 ctgcgacgct ccaggagctt ccacttgaca cccgtcttgc catagcccaa gagcctctcc
      361 aatctgacct cgccatcgtc gtacttaacc acgtgtcgac gtttctcacg gccagacaca
      421 actcccttaa accattttcc ttcggcaggc caccaggtaa tgatcctctt gccaaccaag
      481 tcattgggta gcatcattgg gtcagggtcc acttcgactt cttgatcatt gccaggctct
      541 tcctcttcca attccacttc gcccacctcg cgtcccttgt agggcttaag gcgctccaca
      601 tggacctttt cacgtgcacc accaggcttc gaaatagtga cgtaatttcg gccatgcaca
      661 tcaccaacca cgtagggtcc atcgaacttg ggatccaact tagtcgaatc agcatcgcgg
      721 cgatcaagcc aaactttttc gccttgtttg taggcgggcg ccttcccccc actgaaagta
      781 gcgcggcgtt cctgcgcgcg tttcatctca tcgctcgctt cccgtgctac acgaaatcct
      841 tcttggagat cgatgagcaa ttcattggta aactcggcaa cagcgcgctc ttcaggcgct
      901 cgtgctccaa gaaccacgtc caaaggcaat ctcgccaaac gcccaaaaag taactcaaag
      961 ggcgccatct tcgtagccgc atgcttcgag ttgttgtacg caaaggtaat gaagggaacc
     1021 ttccaatccc aggaagactt gtcgccgtcc atgcacgcgc gcagcgactg cgccacagtg
     1081 ccattccagc gctccaccat tccattcgcc tgcggtcgat acgctgtcgt aaacaactta
     1141 tgcgtacccg ccatcttgca aatcgcattg accacttcag aggcaaaggc agggccgttg
     1201 tcagtgaaga gttctcgagg tgccccaaac ttggtcacga tcttgtccac gaactccctc
     1261 gccacagtct cggcggtgca gtccaccgtt gggacagcca ccacataccg cgtgaagtag
     1321 tcggtaaaga cgagtaagta gcgattacca cgctccgtct tgtccacagg gcccaagaag
     1381 tcggctgaga ctcgctgcca gcggtcaccc acaaccgtcg cttcccactt gccctcgttg
     1441 gagacctttg gcttcttagc acgcgcgcac gcctcgcacg tctccacgta gtgttttgtt
     1501 tccgcatatt gattaggcca ccaatagcgc tcttgaatgt tcgcgtacgt cttctcgatg
     1561 ccaaagtgcg ctcccacgag cgagtcgtgg tgctccaata agacgtcgcg gcgcaaagag
     1621 ttgggtacaa ccagcctctt gctcttgtcc ttcttcccgc ggaagtagaa cagcttgcca
     1681 tcgtcgatga ccaaatatgt agccacttgc cgatataatt tgagcgcatc tggatccttg
     1741 ggctcgtctc cattctcgag cgctcgcatt atcggtccca aggtcgcgtc ttggcgctga
     1801 gcttccccga gctcgagatc cgcgtcaatc gcgaggatct gctcttgccg cgagagcata
     1861 tccggcaact ccatcatttt accagggcga tgctgcacct caaactcgag ttcttgcagc
     1921 gccatcaccc accgcgccac tcgaccgctt gtctcgcgcg ccttgagcac gctgcgcagc
     1981 gcactgtgat cggtctccac cacaaacttg tttccatgca ggtagaacca ccactctttc
     2041 actgcgcgta ccacggcgag tgcttctttc tcagtcgtcg tgtagttgcg ctccgctttg
     2101 gtcagcttct tgctcacgta cgccacgggc aaaagctctc ccaagtgctc ttgaagcaac
     2161 acgccaccac tcgcgtaatc ggaacaatca gtccgaacga cgaaactctt cgttggatcg
     2221 ggaagacgca gaatcggagg cgacgtcaaa cgaccaagga gctcctcgta cgccgactgt
     2281 tgctccttgc cccacgcgta aggctcatcc ttcttcgtga gatcagtcag cggcttagcc
     2341 aaccttgcgt agctttcgat aaatcttcgg aagtatccaa cactgccaag gaaccgttgg
     2401 agctcctttc cattccgcgg cggcgcaagc ttttttactg ccgccacctt ctccatgtcc
     2461 aagcccagct tgccattccc aaccacgtgg ccgaggaagg gtaattcgct taccgcgaag
     2521 atgcacttct tgcgcttcac ctgaaatcct ttatcacgta agcgactaag tacttccgcc
     2581 agatgcttgc catgctcctc cattgtcttt gagtagacaa tgatgtcgtc caagtaaacg
     2641 aatgcgaact cgccaatgat gccttccaag cactcattca tcacgcgctg aaacgtcgta
     2701 ggtgctccag tcagtccaaa gggcatcctc ttaaattggt acttaccttg cttggtaata
     2761 aacgccgtct tgtggcgatc cttattcgct aacggaattt gccagaaccc actctgcaaa
     2821 tccatcttgg acataacccg ggcagcaccc agactgccca agatctcatc gatgcgagga
     2881 aggggatacg gatccttgcg ggtaatagcg ttgagtttgc ggtaatccac gcaaaagcgc
     2941 cacgagctgt ctttcttacg agccaacacc actggagatg cgtactcgct tgttgacttc
     3001 tcgatcactc cctgatccaa catgtccttg agactcgctt cgataatctc gtcctgctta
     3061 ggagaggttc gtctgaggcc atcgaccaca ggctcttccg tcgtgagcgt gacataatgc
     3121 ttcgtcacca ctccgcactc cttgatcggc gcatcaaaga catccttgaa cttctccaat
     3181 accttggtaa tctgcactgg tatctcgacg acctccttcg ggacatccac gtccaccatc
     3241 tcggtactcg ccacaacctc gtcgcaatcc accacaggca acactagggc accattgtcc
     3301 cagtccactt gcgctttgac ttgcttcaaa gcgtctcggc cgagcaaaca ttgctcgcca
     3361 ttcaccaact tctccaagac catggcccgt acagaagcca cgccaccatt ggggaaattg
     3421 accacgagtc gaagccaacc gagcatttcc gtcgccctac catcggccaa ctttgctcca
     3481 atcttcgact cttcccagtc cttccgagca cacaaccgct cagccaagac tgccgagatg
     3541 accgtcgagg aagcaccagt gtccacgtaa gccaccacag gtactcgccc actcgaattc
     3601 acgcccatct ccaccgatac ccgaatcggg gtatccaaac catcgtcagg atccacctgc
     3661 gcgataggtt tcttgtcggc tccacccgac tccttggcct tgaacctctc ggatctcctc
     3721 acttccttgt tcttaggaca ctgaggtcgg aggtgaccaa tttcaccaca attatagcag
     3781 cgcctcggtt cattggtacc cgtctgaggc ttcctcgctt gcggttgagg cttctcttgc
     3841 tgctgcgaag caatctctga gccttggaga tctcgcgcag acttaacgag ttctacgaat
     3901 tttcttcctt ccttcggatg cctcagctta atcgtcgcat gcaactcctt gggaagtcct
     3961 ttaatcgcgg cttccaagaa catctggcca tcttcggtca atgcgtgctc atctcgcttc
     4021 atcaattcca cgtacttgag gtaaagctcc ctcttttcga tgaggtagcc ctcgagggtc
     4081 tcatccttat tcttcctcct agccaggaag gcttggagac gcttgtgagc agtatcgttg
     4141 aaggagaatc tcttgcgcag ctcacccacc aaccattcct ctgtcatgtc ctcggaattc
     4201 ctcgggagag acttcgcctc tcggcactca cgcatgactc gcgtcaagat ctcttgggcg
     4261 aggaccctca acaaggccag gtatcgctca cttccgctga gcgcccatgc gtcggcactg
     4321 gcagtgaagc cagaatacca gtcctcaaag tccttgacgt tgtaatcgaa cttggtaagg
     4381 ttctcggtag cgaacttgat gttgt
//
LOCUS       DATMTI010000127         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_47400, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000127 DATMTI010000000
VERSION     DATMTI010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47400"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..925
                     /locus_tag="VN039_02125"
     CDS             149..925
                     /locus_tag="VN039_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HWV44814.1"
                     /translation="MNVQNIPRKDKVVKLAFLPKLDALLFFDFKQIEPRLLAYFLAVS
                     IGDTTLAHRISAGEDPYTVIVEGLYGRSDLTDEERQTGKRLFLSLMYGGGTPTVIAQF
                     GVDYKTAKSMTDQFHRAWPGVKMLADLIVDTARKRGYIKSITGRHLRLESEHSAINTL
                     MQGSAAEILRVSLERVHEYTESNLMASHIVLNVHDEIIMDVVRPEIPELVTEVPRMMT
                     DFPEINEIVPIETDCEISWTNWAEKVPYEEDNERVLERAG"
     gene            897..1325
                     /locus_tag="VN039_02130"
     CDS             897..1325
                     /locus_tag="VN039_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44815.1"
                     /translation="MSEFLNEPDESPEDDDELTADEEAFLNDLPPAEQQFIRDVAGLD
                     PEEDAEAIENRMRRLDQDRMNDSRREWKAAYGFDHECSCATDVESGNVREVPVCYLGA
                     CSDAFDELRRVRAFLYCIATAPTKEAATLKALAAEAFHGR"
     gene            1325..1471
                     /locus_tag="VN039_02135"
     CDS             1325..1471
                     /locus_tag="VN039_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44816.1"
                     /translation="MKGYHKVKVFVIDGQEEVDLPEGWKPFAAEVGQYRTRVIARKWE
                     RTGE"
     gene            1484..1921
                     /locus_tag="VN039_02140"
     CDS             1484..1921
                     /locus_tag="VN039_02140"
                     /inference="COORDINATES: protein motif:HMM:NF039184.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP diphosphohydrolase domain-containing
                     protein"
                     /protein_id="HWV44817.1"
                     /translation="MPPGEEVCVLSDEGWQLVSDGFTDYIGRFQVKDWGQRMEFESGM
                     VRDVTEGKTRFDLVLDGPMFRRWAEHLTKGAEKYDARNWMKARGGAEYERFRESALRH
                     FVQWYYGDADEDHAAAVFFNINGAEYVRDQGDYDTRDLADGFS"
     gene            1908..2351
                     /locus_tag="VN039_02145"
     CDS             1908..2351
                     /locus_tag="VN039_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44818.1"
                     /translation="MASRKRHIVAVDLDGTLIDSAWPAMGAWKQDAVWAMQEFLKRGY
                     PVIVFTIRISPYWLDGTRRDPEEAEQDTQQVRALLDDAGLHGVSIWTKEGKPPYSILI
                     DDKAVTFPTPGSSHSWRRIVEKVLVKLGAEDPLYEDGEWGWEGAS"
     gene            2477..2626
                     /locus_tag="VN039_02150"
     CDS             2477..2626
                     /locus_tag="VN039_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44819.1"
                     /translation="MQVSHLAPLVQLENRELRDEIRVLREVIRKQGEFILSLKHEIRD
                     FLGNA"
     gene            2619..>3219
                     /locus_tag="VN039_02155"
     CDS             2619..>3219
                     /locus_tag="VN039_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44820.1"
                     /translation="MPDTEAVANWFEEERGISRETLRHFGVTVRDDGAVVLPYPNGEK
                     IRKGIPHGDREFYFTSGRKPDLFNQDEASKMTVFLVEGETDTMRLWQELGSDRQYGVV
                     GLSGIETWRDDLAGAFTGQRVYVVLDNDRDYNVQARVDTAWRNIRASLGGKARRVRLP
                     GDVKDICEFFDAGYILDHLFRAAESGTGQSRWHPLDLTQA"
BASE COUNT          726 a          845 c         1035 g          613 t
ORIGIN      
        1 attcgggttg tctggtgtgg tatgaaatgc ccgatgggaa atctgcgtac aagcctacgc
       61 acgaatgttc gtgtggcttc aagtatgtag gagataaaga taagcagacg gagcttgagc
      121 acgttctgaa ggcggaaggg ctgtatgcgt gaacgtccag aacattcccc gcaaggacaa
      181 ggtggtgaag ctcgccttcc tacccaagct cgacgccctg ctgttctttg acttcaagca
      241 gatcgagccg aggctgctgg cgtacttcct cgccgtgtcc atcggagaca caacgcttgc
      301 ccaccgaatc tcggcgggtg aggatccgta cacggtcatc gtggaagggc tgtacgggcg
      361 cagtgacctg acggacgagg agcggcagac aggcaagcgc ctgttcctgt cgctcatgta
      421 cggcggcggg acaccgactg tcatcgcgca gttcggcgtg gactacaaga ccgccaagag
      481 catgaccgac cagttccaca gggcatggcc cggtgtgaag atgctcgcag acttgatcgt
      541 ggacaccgct cggaagcgcg gttacatcaa gtccatcaca ggcagacacc tcaggctcga
      601 atctgagcac tcagcgatca acacgctgat gcagggttca gccgccgaga ttctcagggt
      661 gtcgctggag cgcgtgcatg agtataccga gagcaacctg atggcctcac acatcgtgct
      721 gaacgtacac gacgagatca tcatggatgt agtccggcct gagataccgg agctagtcac
      781 ggaagtcccg cgcatgatga cggacttccc agagatcaac gagatcgtgc ccatcgaaac
      841 agactgcgag attagctgga cgaattgggc cgagaaggtt ccatacgagg aggacaatga
      901 gcgagttctt gaacgagccg gatgagagtc cggaggatga cgacgagctg acagccgacg
      961 aggaagcgtt cctcaacgac cttcccccgg cagagcagca gttcattcga gacgtagcgg
     1021 gacttgatcc cgaggaggac gcagaggcaa tcgagaatcg gatgcgtcgt ctagaccagg
     1081 atcgcatgaa cgattcccgc cgcgagtgga aggcggctta tggcttcgac catgagtgtt
     1141 cctgcgccac tgatgttgag tcggggaacg tgcgtgaggt tccggtctgc tacctcggtg
     1201 cctgctcgga tgccttcgat gagttgcgtc gagtgagagc gttcctgtac tgcatcgcta
     1261 cggctccgac gaaggaagcc gcaacgctca aggcgctggc agccgaagca ttccacgggc
     1321 gctaatgaag ggctaccaca aagtcaaggt gttcgtgatc gacggacaag aggaggtgga
     1381 tcttcctgaa ggatggaaac cgtttgccgc agaagtcgga caataccgaa cgcgagtcat
     1441 tgcccgaaag tgggagcgca ccggagagtg aaaggaagtg tgtatgccac caggggaaga
     1501 agtgtgcgta ctgtctgatg agggatggca gcttgtgagt gatggattca cggactatat
     1561 aggtcgcttc caggttaagg attggggaca gcgcatggag ttcgagtccg gcatggtgcg
     1621 tgacgtgacc gagggcaaga cgcggttcga tctcgttcta gacgggccta tgttccgccg
     1681 ctgggctgag catctgacca agggcgcaga gaagtacgac gctcgtaact ggatgaaggc
     1741 gcgcggggga gctgagtacg aacggttccg tgagtcagcc cttcgtcact tcgttcagtg
     1801 gtactacgga gatgctgacg aagatcacgc cgccgccgtg ttcttcaaca tcaacggagc
     1861 agagtacgtg cgtgaccaag gcgactatga cacgagggat ctagctgatg gcttctcgta
     1921 agcgtcacat cgtcgccgtc gatctagacg ggacgttgat cgatagcgca tggcctgcca
     1981 tgggagcctg gaagcaagat gcggtgtggg cgatgcagga gttcctcaag cggggatatc
     2041 cggtgatcgt cttcaccatc cgaatcagtc cgtactggct cgacggtaca cgccgcgatc
     2101 ccgaggaagc tgagcaggac acacagcagg tacgcgcgtt gctagacgac gcgggcctgc
     2161 acggtgtctc gatctggacg aaggaaggca agccgcccta ctccatcctg atcgatgaca
     2221 aggcggtcac gttcccgact cccggctcca gccatagttg gaggcgcatc gtggagaagg
     2281 tgctcgtcaa gctaggcgcg gaagatcccc tgtacgaaga cggcgaatgg ggatgggaag
     2341 gtgcgtcctg atggcagagg aaagttccgg cttgcaccgg ggatcaggct tattcgtctt
     2401 cccacctgtg ccttttgtaa acacagtgct accgagtgca ggtgcggacg cccaaagctc
     2461 tacgaaggaa ggtgatgtgc aggtatctca tctagctccg ctcgtgcagc tggagaacag
     2521 agagctgcgc gacgagatca gggtactacg ggaagtcatc cggaaacagg gagagttcat
     2581 cttgtccctg aagcacgaaa tccgggactt cctcggtaat gcctgacact gaggcggtcg
     2641 ctaactggtt cgaggaagag agaggcatca gccgcgaaac cctgcgtcac tttggagtga
     2701 cagtgcgcga cgatggtgca gtcgttcttc cctatccgaa cggcgagaag atccgaaagg
     2761 gtattccgca tggcgaccgt gagttctact tcacatcagg ccgcaagccc gatctattca
     2821 accaggacga ggccagcaag atgacggtgt tcctggttga aggcgagacg gacacgatgc
     2881 ggctgtggca ggaactcggc tcagaccgtc agtacggcgt tgtaggtctg tccggcatcg
     2941 agacatggcg cgatgatcta gctggagctt tcacaggtca gagggtctac gtcgttctcg
     3001 acaatgaccg ggactacaac gtgcaggcca gggtggacac ggcttggcgc aacatccggg
     3061 catcgctcgg aggcaaggcg cgtcgtgtgc ggctccccgg tgacgtaaag gacatctgcg
     3121 agttcttcga tgcgggctac atccttgacc atctgttccg cgccgcagag agcggtacgg
     3181 gacagtcccg ctggcatcct ctagacctga cgcaggctc
//
LOCUS       DATMTI010000128         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_768741, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000128 DATMTI010000000
VERSION     DATMTI010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768741"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VN039_02160"
     CDS             complement(<1..341)
                     /locus_tag="VN039_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV44821.1"
                     /translation="MQHLSIHGTELAYKVVGRGEPYLVMHGGLGFDHTMLHPGLDRLA
                     DTCQLVYYDHRGHGQSGRPPLDMLTWEQLAHDAEGLRRHRGYQRICIIAHSIGAFPAL
                     EYALHYPRVLHR"
     gene            complement(512..769)
                     /locus_tag="VN039_02165"
     CDS             complement(512..769)
                     /locus_tag="VN039_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44822.1"
                     /translation="MLTIRISVAWLIAVICLGTLGLLLLLVQPFTVKAQEPKRFQYQI
                     VEVIPDTETMQTRLNEFGSNGWELVGFAMGNMTNPRMIFKK"
     gene            complement(794..3448)
                     /gene="polA"
                     /locus_tag="VN039_02170"
     CDS             complement(794..3448)
                     /gene="polA"
                     /locus_tag="VN039_02170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWV44823.1"
                     /translation="MPHTDKRLTLYLIDGSAYIYRAFFALPPLSNSKGFQTNAVYGFT
                     TMLLKIMREHRPDALAVVFDEKGPTQRHEEYQAYKAQRPAMPDAMSAQVPYIHRIVEA
                     LAIPALRLAGYEADDIIGTLARKAEQADRDVAIVTSDKDMFQLLTPHVKIYDPVKDKW
                     IGEQECRERFGVEPVLVPEIMGLMGDAIDNIPGVKGIGEKTATKLIGQFRTIEELLRR
                     VEEVTPARTKTMLIEQADNARLSRRLAMIQTDCPVEFEIGALQIKPAHDDRLAELLRE
                     LEFTTLLKSIQPTSPQASIHQASVETIESEAAAQAFVVRLNKSRTIAVQCLLSGPPGV
                     RADVQGIALSSEDKTAFVPLDVHTYMRPITALFHETGRLKAVHDLKLTLLAFHRIGVT
                     LAGPYFDTMIADYLLNPNRRDHQLDTLALELLDHRLGAQAKEPSPPASLFQVETASQE
                     QTAERAAVIAKLTHVMFDRLRDQGSLRLFQEVEMPLVPILADIEQNGFLLDVDGLHAL
                     SKELERELDRMMDTIARLAGGEFNINSPKQLATVLFERLGLKPVRKTKTGYSTDEDTL
                     TQLAAQHELPAQILNYRSLSKLKSTYVDALPELVHPETKRLHTSLNQTVAATGRLSST
                     DPNLQNIPVKGDYGLRIREAFIVPKGHELLCADYSQIEPRILAHLSQDPRLLSVFTKA
                     EDIHMATAMEIFGLPSSQITRDMRRAAKTVVFGIVYGISPFGLSQNLGVPQAEAKKYI
                     ETFFERFPAVRALMDRNIAEGREKGYTTTILGRRRPIPELQSSDPIQRGIGERMAVNS
                     PIQGSAADLIKVAMINVHKKLHEELPHAKMILQVHDELIFEVPDHDLEEAKRLVRQEM
                     EGVGEQLGLSVPLKVDLGIGKNWRVAHP"
     gene            complement(3542..>3987)
                     /locus_tag="VN039_02175"
     CDS             complement(3542..>3987)
                     /locus_tag="VN039_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HWV44824.1"
                     /translation="GCRPMDWLLSAVQQYVSTETLVTLTALSVVFFVGSLIAIPFILV
                     RLPTDFFDTRVPRHWMENHHPVLRLLGHIVKNVVGAIFLFAGFLMLFLPGQGILTMLI
                     GVTMLDFPGKRRMEAKMIGQPAVLSTINNMRQKFGKPPLTIAPGQ"
BASE COUNT          857 a         1070 c         1125 g          935 t
ORIGIN      
        1 cgatgaagta ctcggggata gtggagcgca tattcgagcg cggggaaggc gcctatagaa
       61 tgcgcaataa tgcaaatccg ttggtacccg cgatgccgcc gaagtccttc ggcatcatgc
      121 gccaactgtt cccaggtgag catatcgagc ggtggacggc cggattgccc atgaccacgg
      181 tgatcgtagt agacaagttg gcaagtgtct gcaaggcgat ccaatcccgg atgcagcatc
      241 gtatgatcga accccaaccc gccatgcatc actaaataag gctcacccct ccctacgact
      301 ttgtaagcga gctctgtccc atgaatcgac aagtgctgca tgagatattt ttaagacaag
      361 aataggggta tctcgtacct actcgtcaat tttgccatcc tcatagatgc cccactcacc
      421 agttctcact gacacgagat caactatgag cagaagcaac aggccaggct gcttaccacc
      481 gcttctattt gtgcccacca gccgctgggc gtcacttctt aaagatcatc ctcggatttg
      541 tcatgtttcc catcgcgaac cctaccagtt cccagccgtt ggagccgaac tcattcagtc
      601 tggtctgcat agtctcagta tcgggaatca cctcgacgat ttggtattga aatcgtttcg
      661 gctcttgggc cttgaccgtg aatggttgaa caaggagcag caaaagccca agagttccaa
      721 ggcagataac tgcgatcagc cacgcgacag aaattcgtat ggtcagcata tgtcacctcg
      781 cattgcttgt gagttaggga tgcgcaactc gccagttttt gccgatgccg agatccactt
      841 taagaggcac cgacaagcct aactgttcgc cgacgccttc catttcttgc ctcaccagtc
      901 gcttcgcttc ttccagatca tgatccggca cttcgaagat caactcgtcg tggacttgga
      961 ggatcatttt cgcatgcggc agctcttcgt ggagtttctt gtggacgtta atcatagcga
     1021 ccttgatcaa gtctgccgcg gaaccttgga tgggactatt taccgccatg cgctcgccga
     1081 tgccacgttg tatcggatcg ctactctgaa gttcaggaat cgggcggcgg cggccaagga
     1141 tcgtggtggt gtagcctttc tcccgtcctt cggcgatgtt ccgatccatc agggcacgga
     1201 cggctgggaa tcgttcaaaa aaggtttcga tgtatttttt ggcctcagcc tggggtacgc
     1261 cgagattctg ggaaaggccg aacggactga ttccatagac gatgccgaag acgacggtct
     1321 tagccgcgcg gcgcatatct cttgtaatct gactcgacgg caggccgaag atttccatgg
     1381 ccgtcgccat gtggatgtct tctgccttgg taaaaacgga cagcaatcgc ggatcttgcg
     1441 agagatgggc aaggatacgc ggttcgatct ggctgtagtc ggcacagagc aattcatggc
     1501 ctttaggaac gatgaacgct tcgcggatgc ggagtccgta gtctcctttc acggggatgt
     1561 tttggagatt gggatcggtg gatgagagcc gtcccgttgc ggcgacggtt tggttcagcg
     1621 aggtatgtag acgtttggtt tccggatgca ccagttccgg caacgcatcc acataagtcg
     1681 acttgagttt gctgaggctt cgataattca gtatttgtgc gggtaactca tgttgtgccg
     1741 caagttgtgt gagcgtatct tcatcggtgg agtaaccggt cttagtttta cggacaggct
     1801 tcaggccgag cctttcgaag agcactgtcg ccagctgttt cggagaattg atgttgaact
     1861 cgccgccggc taaccgggcg atggtgtcca tcatgcggtc gagctcccgt tcgagctcct
     1921 tgctcagcgc gtgcaacccg tcgacatcga gtaagaaccc gttctgctcg atgtctgcca
     1981 ggatcggtac gaggggcatt tcgacttcct ggaacagtct cagactcccc tgatctcgca
     2041 gccgatcgaa catgacgtgc gtgagcttcg cgatcaccgc cgcgcgttcc gcggtttgct
     2101 cctgcgaagc ggtctccact tggaacagcg atgccggtgg agatggttct ttcgcctgag
     2161 caccgagccg atggtcgagg agttcaagag ccagggtatc aagttgatgg tcccgtcgat
     2221 tgggatttag aaggtagtcc gcaatcatcg tgtcaaaata cggcccagca agcgtgacac
     2281 cgatcctgtg gaacgccagg agcgtcagct tcaagtcatg cacggccttc agtcgaccgg
     2341 tttcgtgaaa gagagcggtg atggggcgca tgtatgtatg cacatcgagc gggacaaagg
     2401 cggtcttgtc ctcagacgac agcgcgatgc cctgcacgtc ggctcgtacg ccgggcggac
     2461 ctgagaggag acattgaacg gcgatggtcc ggctcttgtt cagccgtacg acaaaggctt
     2521 gcgccgcggc ctcactttcg atcgtttcca cggatgcttg atggatcgaa gcctgtggcg
     2581 aggtgggctg gatggatttc aatagtgtcg taaattccag ttctcggagc aactcggcga
     2641 gccggtcatc gtgcgccggc ttgatctgta gagcccctat ctcgaactcg actggacaat
     2701 cagtttggat catggccagg cgacggctca gtctggcgtt gtcggcctgc tcgatcaaca
     2761 tggtcttggt ccgggccggc gtcacttctt ccactcgtcg gagcaattcc tcgatcgtcc
     2821 taaactgacc gatcagcttc gtcgccgttt tttccccaat gcctttcacg ccgggaatgt
     2881 tgtcgatggc atcgcccatg agacccatga tctccggaac cagtaccggt tcaactccaa
     2941 accgttcgcg acattcctgc tcgccgatcc atttatcctt gaccggatca taaatcttca
     3001 cgtggggtgt gagcagctga aacatatcct tgtcgcttgt cacgatagcg acatcgcgat
     3061 cggcttgttc ggccttccga gccagtgtgc cgataatatc gtctgcctcg tatccggcca
     3121 gtcggagagc aggaatcgcc agcgcttcca caatgcggtg aatatagggc acctgcgcgc
     3181 tcatggcgtc cggcatggcg gggcgctggg ccttgtatgc ttgatattct tcatgccgtt
     3241 gtgtagggcc cttctcatcg aagaccacgg cgagagcatc aggccggtgc tctcgcatga
     3301 tcttgaggag catggtggta aacccgtaaa cggcattggt ttgaaaacct ttcgagttcg
     3361 acagcggcgg taaggcaaag aaggcccgat agatataggc gctgccgtcg attaggtaaa
     3421 gggtgaggcg tttgtcggtg tgcggcattt cagtgtagcc ttgttgagtg ctgagttctg
     3481 agagccgagt cgagaccagc cgggaaatct cagcactcag tactttcgtc tcagcactgt
     3541 cttattgacc cggcgcaatt gtgagcggcg gctttccaaa cttctggcgc atgttgttga
     3601 tggtgctcag gacagcgggc tgtccgatca tcttggcttc catcctccgc ttgccgggaa
     3661 agtccagcat cgtcaccccg atcaacatcg tgagaatgcc ttggcccggc aagaacaaca
     3721 ttaagaaacc cgcgaacaga aaaatagctc cgacaacatt cttgacgata tggccaagca
     3781 agcgaagcac gggatgatga ttttccatcc aatgccgcgg cacacgggtg tcgaagaaat
     3841 ccgtcggaag ccggacgagg atgaacggga tcgcaatcaa tgatccaacg aagaagacaa
     3901 ccgagagagc cgtcagggtc acgagggttt cggtcgagac atactgctga accgccgaaa
     3961 gaagccaatc catcggtcgg catccta
//
LOCUS       DATMTI010000129         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_641692, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000129 DATMTI010000000
VERSION     DATMTI010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641692"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2195)
                     /locus_tag="VN039_02180"
     CDS             complement(<1..2195)
                     /locus_tag="VN039_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV44825.1"
                     /translation="MRSNKKLPLKSDKSQATRGHKRGVVGYSRKTALLEEAITQMNAG
                     KYGRSSAALKELLALDPHNMEARRLFATLHLRLGSLIPARQAFDSLISEAFQRQDYWL
                     AESLLREYLAAGPRCVPFLEKLGALYQEKGNALEAVGEYGKAIDILIEDPDPDNPHHA
                     AQLYAKIRELAPASPVAFRLASSFDTQTGELLTRQSGDSGQSTIAPSPDLSEGGQGIQ
                     AGSEPMDGVMPWEIQDFQATAPETLKVADVSDSDVSDSSGSLIPPTITDLPAPQGPTL
                     EGDVVPSDPPLREQTANDASEVRNDVPSTESSETQHGPDPAVPTGGPAAVQEQDRLSA
                     EAEPEAPYDLDVSGSSTVSTPIESADASEPPTSSVQSIEAAGVTPLSQIEEISASTYL
                     DTEPPTAGPIEVEDVQPSIHKSVETDSKEVITEPPPDVRAPSSPESVAANEISEPWKQ
                     PGFSWESVFNSAWKFGSDHSANPSPPVVTQTDVDEVHTPASAEPPLQELALESRAVEI
                     PEREAPTSLGEQTPSGSPIAPMPWDQVQESVISIIPSQTDQPAAERAGSTVDRSAGEV
                     ESLSKVSDDQAQPPIFRISPAAEMETFSIAPAIQTPVSPEPEIPAADVKPVSIQTESD
                     FTFVKPEQPPAASSLTMDELPALVVPMAAIAPPIFESESLTDDRVVPTSETLSKETED
                     IPISPPLPERQRIRELPPEIATTPHLAEEPTEAHTHERQPQASASVEKRT"
BASE COUNT          448 a          609 c          654 g          564 t
ORIGIN      
        1 gttcgtttct caacagacgc cgatgcttgc ggctgccgtt catgggtatg cgcctcagtc
       61 ggctcttcgg cgagatgagg agttgtcgct atttcaggcg gcagttcacg aattctctgt
      121 cgctctggta acggcgggga aattggaatg tcttcggtct ccttgctcag cgtctcgctt
      181 gtaggaacca ctctatcgtc tgtgagcgat tcgctctcga aaatcggcgg cgctatcgct
      241 gccatgggca ctactaacgc tggcagctca tccatcgtca gagagctcgc cgccggcggc
      301 tgttccggtt ttacaaaggt gaagtcgctt tctgtctgaa tggagactgg tttcacatcg
      361 gcagcaggga tctccggctc aggcgagacc ggcgtctgta tggctggagc aatcgagaac
      421 gtctccattt ctgcagcggg actaatacga aagatcggag gttgcgcttg atcgtcacta
      481 actttggata gcgactcaac ttcaccggcc gatcggtcta ccgttgaccc cgctctttca
      541 gctgccggct gatctgtttg cgaggggata attgaaatga ccgactcctg aacttgatcc
      601 catggcatcg gtgcaatcgg agatccagaa ggggtttgtt ctccaagcga tgtcggtgct
      661 tcacgctccg gaatttcaac cgcccggctt tctaacgcaa gttcctggag aggtggctct
      721 gctgacgctg gagtatgtac ttcgtcgaca tccgtctggg tgactaccgg cggggaggga
      781 tttgctgaat gatcacttcc gaacttccat gcactattaa acacggattc ccatgagaag
      841 cctggttgct tccatggctc tgaaatctca ttggctgcaa ccgactcggg actcgatgga
      901 gcacggacgt ctggaggcgg ttcagtaatc acttccttcg aatcggtctc gacagatttg
      961 tgtatggagg gttgcacatc ctcgacctct atcggcccgg cagtcggcgg ttcggtgtcc
     1021 aagtaggtgg atgctgatat ctcttcgatt tgggataagg gcgttacgcc ggcagcttca
     1081 atagactgta ccgagctggt cggaggttcc gatgcatcgg cggactcgat gggtgtggaa
     1141 accgtggatg acccactgac atctagatca tagggtgctt ccggctcagc ctcggccgac
     1201 aatctgtctt gttcctgaac tgcggcagga ccacctgttg gcacggctgg atccggtcca
     1261 tgttgtgtct cggaactctc ggtagagggc acatcatttc gcacttcact cgcgtcgttg
     1321 gctgtctgct ctcgtaaggg tggatcggat ggcaccacat ctccctcaag ggtcggccct
     1381 tgaggggcag gaaggtccgt gatcgtcgga gggataagag accccgatga gtcggataca
     1441 tccgagtcgg atacatcggc tactttgagc gtctccggtg ccgtcgcttg aaaatcctga
     1501 atctcccacg gcatcacccc atccatcggt tccgaacctg cttgaatacc ctgacctccc
     1561 tcggacaggt cgggtgatgg agcaatggtc gattgtccgg aatcgccgga ctgccgagtc
     1621 agcagctccc cggtttgcgt gtcaaaggac gaagccaatc gaaaggccac cggactcgcg
     1681 ggagcaagtt ctcgtatttt tgcgtagagt tgagcggcat ggtgtggatt gtcgggatca
     1741 gggtcttcga tcaagatatc gattgccttg ccgtactcgc caacagcctc gagtgcattt
     1801 cctttttctt gatagagcgc cccaagtttc tctaggaaag gaacacatcg agggccggcg
     1861 gctaagtatt ctcggagcaa cgattctgcg agccagtaat cttgccgctg gaacgcttcg
     1921 ctgatgagtg agtcaaaggc ttgtctcgcc gggataaggc ttccgagacg aaggtggagg
     1981 gtagcgaaaa gccgtcgcgc ctccatgttg tgcggatcaa gtgccagtaa ttccttcagt
     2041 gcagcagaag aacgcccata ttttccggca ttcatttggg tgatggcttc ctcaagaaga
     2101 gccgtcttcc tgctgtagcc gaccacacca cgcttgtgac cacgggtggc ctgggattta
     2161 tcagatttta aagggagttt tttattcgag cgcacgctac aaccttagca gatccctcta
     2221 tctgggagcc tgcatcagcg gctcaatccg ctctccccga ctcccttgat gttgg
//
LOCUS       DATMTI010000130         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_132482, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000130 DATMTI010000000
VERSION     DATMTI010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132482"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1578)
                     /locus_tag="VN039_02185"
     CDS             complement(<1..>1578)
                     /locus_tag="VN039_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44826.1"
                     /translation="VMTDTAVVLTLEKGHATAKVEGTRVTFSPHQCLAFDHGPDDVAC
                     LRALGAHLLTLPGLALVGSKRFIDYLLHHVPGLDSRIVGTALYENLAERHQLDGLATV
                     KLGALPCETQTAFLCETLAFPRMQMMKQLPQSVTIVDLTALTDVAPDVIPARGWTPLP
                     RNIYPIEIPEIQIRKDLDVVIVDCPSRNLSLMPNGLGYLNNALKKTAVSFQIVDLDII
                     SYHRFHVHRLFDMGGCITLPGDLVLPEDPWQAEHYDLWTATGGGASGPTGRNEVLEFF
                     RPVIDETIAALVAARPRVLGLSIQGCNEAAAREVALGVKARLPDITIVVGGFSCYNAD
                     VGRRAFPECDYMCIGEADLTAGPLLEALARGERPFNQPGVLSRFDTPDYSYLPAPMIH
                     NLDQIEFPKYEWCDLSVYRNFNDYQLTPIIASRGCRWSRCTFCAERFYWRIRTKENFA
                     DELEWLVGRGCHLFMFNESDLGGMPERVMEICDEIIRRGLHRKVKLTGQLRVNRKQDR
                     AFFEKLREANFVALRFG"
BASE COUNT          372 a          459 c          426 g          321 t
ORIGIN      
        1 tcccgaaacg aagcgcaacg aaattggcct ctcgcagctt ttcgaagaag gctcggtctt
       61 gtttcctgtt gacgcgcagc tggcccgtga gcttcacctt gcgatggaga ccacggcgaa
      121 tgatctcatc acaaatctcc atgacacgct cgggcatgcc gccaagatcg ctctcgttga
      181 acatgaagag atggcagccc cgtccgacca gccattccag ttcatccgcg aaattttcct
      241 tggtgcgaat gcgccagtag aaccgttcgg cgcagaacgt gcagcgcgac cagcgacaac
      301 cccggctcgc gataataggc gtcaattggt agtcattgaa gttcctgtac acgctcagat
      361 cgcaccactc atacttgggg aattcgatct gatcgaggtt atgaatcata ggtgccggga
      421 gataagaata atcgggcgta tcgaaacggg acagcacgcc cggctggttg aacggccgct
      481 cgcccctggc caaggcttct aacagcggac ccgccgtgag gtctgcttcg ccgatgcaca
      541 tgtagtcgca ttccgggaat gcacggcggc cgacatccgc gttgtagcaa ctaaacccac
      601 ccaccacgat cgtgatatcg ggcaaccgag ccttgacgcc gagcgcaacc tctcgtgccg
      661 ccgcctcatt acatccttgg attgaaagcc caagcactct tggccgtgct gcgaccaaag
      721 ctgcgatcgt ctcgtcgatg acgggcctga agaactccaa cacttcgttg cgtccggttg
      781 ggccggaagc gcctccaccc gttgctgtcc acagatcgta atgttccgcc tgccaaggat
      841 cctccggcaa aaccaaatca ccgggaagcg tgatacatcc gcccatgtcg aagagccggt
      901 gaacatgaaa ccgatgatag gaaataatat ctagatcgac aatctgaaaa gacaccgccg
      961 tcttcttcaa agcattgttc aaatatccca atccgttggg catcaaggac aggttacgtg
     1021 agggacagtc gacaatgacc acgtccagat ccttcctgat ctgaatctcc ggtatttcga
     1081 tcggataaat attgcgcggc agcggtgtcc acccgcgggc cggaatgacg tcgggtgcca
     1141 cgtccgtcaa agccgtgaga tcgacgatag tgaccgattg gggcaactgc ttcatcatct
     1201 gcatgcgcgg aaacgccaac gtttcgcaga gaaaggcggt ttgtgtttca cagggtaagg
     1261 ctccaagctt aacagtggcg aggccatcca gctggtgcct ctccgcgaga ttctcgtaaa
     1321 gtgcagtgcc gacgatccga ctgtcgagtc cgggcacatg gtgcaagaga taatcgataa
     1381 atctttttga accgaccagg gcaagaccgg gcaatgttaa gagatgagcc ccgagcgcgc
     1441 gcaagcacgc cacatcgtcc ggcccatggt cgaaggccaa gcattggtgt ggactgaatg
     1501 tgacgcgtgt cccctccacc ttggcagttg catggccttt ctcaagagtc agaacgacgg
     1561 ctgtatcggt catcactc
//
LOCUS       DATMTI010000131         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_599105, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000131 DATMTI010000000
VERSION     DATMTI010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599105"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VN039_02190"
     CDS             <1..1086
                     /locus_tag="VN039_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWV44827.1"
                     /translation="ECYYCKLCFNHCPYTPPHHYEIDFPHLMVAWKKHLAAERGVPFR
                     DRLLIMTDIIGRLGSAIASIANRLLENRLVRGLLERLMGIHRDRRLLHFSGETFPRWF
                     GRRTKTAIADPSVRKVALFASCLVNFQATDVGKATVQVLEKNGAQVVVPEQRCCGMPS
                     FDIGDTQAIQQAIRSNVASLYPWVLKGYDVVVPTASCSLMLKREYPELHRDEQTRQVA
                     ERTFDVCEYLMVMKKAGQLTSDFIKKPGRVAYQVPCHLRDQNIGFKSKELMECAGAQV
                     EVIEQCSGHDGAWSAKTEFFLLSMKIAGKAVRAIEQTPADLVASDCPLAGLQLEQAGA
                     SAHARGKAVLHPIQIVRDAYGLPSSKD"
     gene            1155..1751
                     /locus_tag="VN039_02195"
     CDS             1155..1751
                     /locus_tag="VN039_02195"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HWV44828.1"
                     /translation="MKTIGLQDVISYEEYERQRDTFRAKIITLKQRRRISLGPLITMV
                     FENRDTLRFQIQEMIRAERIIDPVKVQDELDVYNALLPQQNELSATLLIEITDEAKVK
                     ENLDQFMGLDHGEKVAIVAGGEEAFGEFEGGRSHETKISAVHFVRFRPTASMKGAIAD
                     LTRPVTIRVNHGGYHQDVPVPGSMREEWLADLKDSAES"
     gene            1828..2601
                     /locus_tag="VN039_02200"
     CDS             1828..2601
                     /locus_tag="VN039_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWV44829.1"
                     /translation="MPPVLLNKRIEFAAAHRYIRTEWDEAKNRAVFGRCYNPPAHGHN
                     YLLEVTVSGEIDPRTGMVVNLFDLKRVLLAVLEEFDHKNLNLDMPYFKERIPTSENFA
                     HVLWTKLASQRDIGILHALRLCEDEDLYAEVTAADGPDVAAVAKRYSFNAIQDSHQGR
                     DWDFYVTVHGTIDPMTGMVTDIGALDRLVQDTVIKTFNRQDLRQVLESETVRGEALTK
                     TIWDRLAGRLSGGTLRNVRLVQTRDLSFDYADLPSRQVS"
     gene            2778..3125
                     /locus_tag="VN039_02205"
     CDS             2778..3125
                     /locus_tag="VN039_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44830.1"
                     /translation="MNDIAVRAAFRNTCADGGNVVRSICWIKLPITYTASGAGSGTRR
                     HVIAHLSGLGEIRAADVGLFGTLLQGLSYSQYEACAEAVPDMFGLSASSVSRQFIRAS
                     AQVLKAFSERRLE"
     gene            3269..>3515
                     /locus_tag="VN039_02210"
     CDS             3269..>3515
                     /locus_tag="VN039_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44831.1"
                     /translation="MCRILAERGGAGLGTDRGLLCVIDGPSAHYSRRFVAKSHSRHAR
                     QAHGTVKKGEYLKQYVERLSGEPAWAILDRVQTIVAAN"
BASE COUNT          795 a          933 c         1043 g          744 t
ORIGIN      
        1 gaatgctact actgcaagct ctgtttcaat cattgtccct atactccgcc ccatcactac
       61 gaaatcgact tcccgcattt gatggtcgcc tggaagaagc atcttgcagc ggagcgcggc
      121 gttccgtttc gggatcgcct actgatcatg accgatatta tcggaagact cggcagtgca
      181 atcgcctcca tcgccaacag gttgttggag aaccgacttg tgcgcggcct tttggaacga
      241 ctcatgggga ttcatcggga ccgtcggctc ctgcattttt ccggagagac gttcccccgc
      301 tggttcggcc gtcgaaccaa gacagctata gccgatccat ccgtccgcaa ggtcgccctg
      361 tttgccagtt gcctcgtgaa ttttcaggcg accgacgtcg gcaaggcgac ggtacaggta
      421 ctggagaaga acggggccca ggtggtggtg ccggaacagc gctgctgcgg catgcccagt
      481 ttcgatattg gggataccca ggccattcag caggctattc gaagcaacgt cgcgtcattg
      541 tacccctggg tcctcaaggg gtacgatgta gtagtcccga ccgccagttg cagtttgatg
      601 ctgaagcgcg aatatccgga gcttcatcgc gatgagcaga ccagacaggt ggccgaacgg
      661 accttcgatg tctgtgaata tttgatggtg atgaaaaaag ccggccaatt gacgagcgac
      721 ttcatcaaga agccagggcg cgtcgcctat caggtcccct gtcacctcag ggaccagaat
      781 attgggttta aatcaaagga actcatggag tgtgccgggg cacaggttga ggtgatcgaa
      841 caatgttcgg ggcatgacgg cgcctggtcg gccaaaacgg agtttttcct gttgtcgatg
      901 aaaatcgccg gcaaggccgt gcgcgcgatc gagcaaacac cggcggacct cgtcgcgtcg
      961 gactgtccgt tggcggggtt acagctggag caagccggcg cctcggccca tgcaagagga
     1021 aaggccgtct tgcatccgat tcagatcgtc cgtgacgcgt atgggctgcc ttcttcgaag
     1081 gactgagtca cgagggctgc ggactgagaa gagccgaacc cggcctcaat gctgggagag
     1141 gagagcgcac atccatgaaa acgatcgggc ttcaagatgt catctcgtac gaagagtatg
     1201 aacgacagcg agataccttt cgcgcaaaga tcatcacact gaagcaacgg cgacggattt
     1261 ctcttggccc attgatcacc atggtgttcg aaaatcggga cactctccgg tttcaaattc
     1321 aggaaatgat cagagccgag cggatcatcg atccggtcaa ggtccaggat gagctggatg
     1381 tgtacaatgc tctgctgccg cagcagaacg agctgagcgc caccctcttg atcgagatca
     1441 ccgatgaggc gaaggtaaaa gaaaatctcg atcagttcat gggactggat cacggagaga
     1501 aagtggccat cgtcgccggc ggagaagaag ccttcggcga gttcgaaggt ggccgaagcc
     1561 atgagacgaa aatcagcgca gtccatttcg tcaggtttcg accgactgcc tcgatgaaag
     1621 gagccattgc agacctcacc cgaccggtga cgattcgagt gaaccatggt ggatatcacc
     1681 aggatgttcc tgtccctggc agcatgaggg aagagtggct tgccgatttg aaggacagtg
     1741 ctgagtctta aatgttgggt gctgagcttt taacgactta tccatccgtg tcacaactca
     1801 gcactcagga ttcagcactg gaaaactatg ccccccgttc tgttgaataa acgtatcgag
     1861 ttcgccgccg cgcatcgcta tatcaggact gaatgggatg aggcgaagaa tcgcgcggtg
     1921 ttcgggcgct gctacaatcc tcccgctcac ggacataact atctgctgga ggtgacggtc
     1981 tccggcgaga ttgatccaag gaccggcatg gtcgtcaatc tgttcgacct caaacgggta
     2041 ctgctcgccg tactcgaaga gttcgatcac aagaacctga acctcgacat gccctacttc
     2101 aaagagcgga ttcccacatc ggagaatttc gcgcatgtgc tctggacgaa attggcctct
     2161 caacgggata tcggcatact gcatgccctt cggctctgcg aggatgaaga tctgtatgcc
     2221 gaggttaccg cagccgacgg tccggatgtc gctgccgtcg ccaagcgata ttcgttcaat
     2281 gccatccaag acagccatca ggggagggac tgggactttt atgtgacggt tcatggaaca
     2341 atcgatccga tgacgggcat ggtgaccgac atcggtgcct tggaccgact tgtgcaggac
     2401 accgtcatca agactttcaa tcgacaggac ctccgtcagg tgctcgaatc cgaaacagtg
     2461 aggggagaag cgctcaccaa gaccatctgg gatcgtctcg ccggtcgtct ctccggtgga
     2521 acccttcgca atgtccgcct cgtccaaacc cgcgacctct cattcgacta cgcagacctt
     2581 ccatctcgcc aagtatctta atatctcgtc catctcgtga accgatagta atccgactca
     2641 ccaacgaagc caaggtctga cagatccgtc ggtgagccct ggaggctccc agcggacgcc
     2701 cggcgagcct gggggcgtga tccaggcggt gatgccactg gcggaagcat tgaataggga
     2761 aatgatcgag ccggcggatg aacgatatcg ccgtacgggc agcctttcgg aatacgtgcg
     2821 ctgatggcgg caatgtggtt cggtctatct gctggatcaa gctgcccatc acctacaccg
     2881 cgtccggggc tggctcagga acgcggaggc acgtaattgc acatttatca ggtcttggcg
     2941 aaatccgggc agcggatgta gggctgtttg gcactctgct gcagggcctg agttatagcc
     3001 agtatgaggc ctgtgcagaa gcggtgccgg acatgttcgg gctgagtgcc tccagtgttt
     3061 cgcggcagtt catccgagcc agtgcccagg tcctgaaggc attcagcgag cggcgactgg
     3121 agtaaaacgg cgtgatcgta ctcgtcctcg acggcaaggg ttttgcctag ttctagattt
     3181 aattagaaag cggggtgctc gtcttagatg tcctgccgac gggcagaaaa tgctggggtt
     3241 tgccagaccg ctacggaaac gcagcgattt gtgtaggatt cttgcggagc gtggtggagc
     3301 agggctgggg accgatcggg gtctgctctg cgtaatcgat ggccctagcg cgcattattc
     3361 acgacggttt gttgcgaaat cgcacagtcg tcatgcgaga caggcgcacg gaaccgtgaa
     3421 gaagggagaa tacttgaaac agtatgtcga acgactgagc ggcgagcctg cctgggcgat
     3481 cttggatcgt gtccagacta ttgtagcggc aaatt
//
LOCUS       DATMTI010000132         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_89967, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000132 DATMTI010000000
VERSION     DATMTI010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89967"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1191
                     /locus_tag="VN039_02215"
     CDS             82..1191
                     /locus_tag="VN039_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWV44832.1"
                     /translation="MKDDPHSRQNAKDAGQRLVSGHASEDPFAAAFKATRMPMLITDP
                     RQPDNPIIFCNTAFCILTGYAVEELVGRNCRLLQGPQTDSAAVDRLRDAVASERDISI
                     DILNYRKDGTKFWNALFVSPVRDVSGVVIYFFASQLDFTNIKSKEAELARARHLAEEE
                     VAKRTADLTEALRAKTALVHEVDHRVKNNLLTIASIVKLQARMTDNEVVERTLMSVLN
                     RVEALSTVQRKLLNDEDVGHFDVADFANTLMLDKIGALKRADIHLTTDLHEVVVPATK
                     ASPLALIINELIDDAIGRGLREGGGELHLEVKRLNGHFLIRVVDTVVPVEVDGEQAAF
                     SKKMLEASLKQLRATIVRTVDERRTDVRVTLSVKD"
     gene            1203..>1541
                     /locus_tag="VN039_02220"
     CDS             1203..>1541
                     /locus_tag="VN039_02220"
                     /inference="COORDINATES: protein motif:HMM:NF009971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV44833.1"
                     /translation="MKILIVEDEALLAMELESEVEDAGHEIVGIAADSKQALSLIEKV
                     SPQFAFVDIQLLDGPTGIDVGQHLASRAVPYVFVSGNLKRIPEDFAGALGAIEKPYTM
                     NGLQNALQFIE"
BASE COUNT          363 a          416 c          438 g          324 t
ORIGIN      
        1 ctgcgtccct gattttttcc agggattgga acaaaagccg agataaccca tttcatgggc
       61 gtgcaatttt caggacgctg aatgaaagac gatccccatt cccggcagaa tgccaaagac
      121 gccggacagc gtctcgtttc agggcatgct tccgaagatc cgtttgcggc ggcattcaag
      181 gccacccgca tgccgatgct cattacagat ccccggcaac ccgacaatcc tatcattttc
      241 tgcaatacag ccttctgcat cctcaccggc tatgcggtgg aggaattggt tggacgaaac
      301 tgccgtctcc ttcaggggcc acagaccgat tcggccgcgg tcgacagact gcgtgacgcc
      361 gttgcgtcgg aaagagacat atccatcgat atcctcaact atcggaaaga cggcacgaag
      421 ttctggaacg cgttgtttgt aagcccggtg cgcgatgtat ccggagtggt gatttacttt
      481 ttcgcctcgc agctcgactt caccaatata aagagcaagg aggccgaact cgcccgcgcg
      541 cgacatctcg ccgaggaaga agtggcgaag agaacggcgg atctgaccga agccctgcgc
      601 gccaaaaccg cgttggtcca tgaggtcgac catcgtgtga aaaacaacct tctgacgatc
      661 gcctccatcg tcaaactcca ggcgcgcatg accgacaatg aggtcgtcga gcggacgttg
      721 atgtcggtgc tgaatcgggt cgaagcgctc agtaccgtcc agcgaaagct tctgaacgac
      781 gaggacgtag gccattttga tgtcgcggat ttcgccaata ccctgatgtt ggacaagatc
      841 ggcgccttga aacgggctga tatacatctc acgacagacc ttcatgaggt cgtggttccc
      901 gccaccaagg catcccctct ggctttgatc ataaatgagc tcattgacga cgccattggc
      961 agggggctga gggagggcgg tggcgaactg cacctggagg tgaagcggct caacgggcac
     1021 ttccttatca gggttgtcga tactgtcgtt ccggtggagg tggatgggga acaggccgcc
     1081 ttcagcaaga aaatgctgga agcctccctg aagcagctgc gtgcaacgat cgtcaggaca
     1141 gtcgatgagc gccgcaccga cgtccgggtc acgctttccg taaaagattg aaaggatatt
     1201 ttgtgaaaat cttgatcgtc gaagacgaag ctttgctggc catggagctg gagagcgaag
     1261 tggaagacgc cggacacgag atcgtcggca ttgccgccga cagcaagcag gctctctcgt
     1321 tgatagaaaa ggtgtcgccg caatttgcct tcgtagacat ccaattactg gacggcccga
     1381 ctggaataga cgtcggccaa catcttgctt cccgcgcagt tccctatgtc ttcgtttcag
     1441 gtaatctgaa gaggatccca gaggacttcg cgggtgcgct tggcgcgatt gagaaacctt
     1501 acacgatgaa cggcttgcag aacgcactgc aattcattga a
//
LOCUS       DATMTI010000133         1881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_175261, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000133 DATMTI010000000
VERSION     DATMTI010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175261"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VN039_02225"
     CDS             complement(<1..327)
                     /locus_tag="VN039_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWV44834.1"
                     /translation="MKTSRMLWLSLVMLVLGAAPACSQNGVEPKGTGAVASAPAELRE
                     GEQKFNANCSTCHGIGGVGTTQGPPFLHKVYEPSHHGDAAFQRAAANGVKAHHWQFGD
                     MPKIDAV"
     gene            complement(363..1061)
                     /gene="queC"
                     /locus_tag="VN039_02230"
     CDS             complement(363..1061)
                     /gene="queC"
                     /locus_tag="VN039_02230"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWV44835.1"
                     /translation="MNGQTSERAVVLASGGLDSTVTAALARRDGYALYLLTIAYQQRH
                     AVEVERARQVATALGAQRHVVVDVDLRAIGGSALTGDMAVPKHRTESERNRDVPATYV
                     PGRNLIFLSLAAAHAEVSEASIIYFGANVIDYSGYPDCRQEFINAVEATLQAGTKTGM
                     QGKGIEIRAPLLRMTKAEIIKLGLILNVPFHLTHSCYDPIGPIACGQCDSCLIRKRGF
                     AEAGVVDPIQYAVC"
     gene            complement(1111..1674)
                     /locus_tag="VN039_02235"
     CDS             complement(1111..1674)
                     /locus_tag="VN039_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HWV44836.1"
                     /translation="MTMKTQAKKKSEAKTKSSATAVKKPQPVITEEAAALKATPEVEI
                     EVPVRPKETAKEREARERRQEVLHKMLIGKRQEIIKEIEESLGQSLTEDQQRRLESAR
                     DVGDQALMDLERELGISLMEMRNRRRQSIDEALTRLHEGTYGICAECGVEISEKRLQA
                     VPFAKLCVECQSRAELLEKIEREEERD"
BASE COUNT          389 a          574 c          475 g          443 t
ORIGIN      
        1 aaccgcatcg atcttcggca tgtcaccaaa ctgccaatga tgagccttta ctccattggc
       61 cgccgcacgt tgaaacgccg catccccgtg atggctcggc tcatagacct tgtgaagaaa
      121 cggtggacct tgagtcgttc cgaccccgcc gatcccgtgg caggttgaac agttggcgtt
      181 gaatttctgc tctccttccc gcaactccgc cggcgcagag gccacggctc ccgttccctt
      241 tggttccacc ccgttttggc tgcatgccgg tgcggcacca aggaccaaca taacgagact
      301 caaccacagc attcgtgatg tcttcataga agtgtgatgg ttatcggaat cggaccgttg
      361 gctcaacata ccgcatactg aatcggatct acaactcctg cctccgcaaa tcctcgtttc
      421 cgaatcaggc aactgtcgca ctgcccgcag gcaatcggcc caattgggtc ataacaacta
      481 tgcgtgagat gaaaggggac attcagaatc agcccgagct ttataatctc cgccttggtc
      541 atccggagca gcggcgctcg aatctcaatg cctttgccct gcatgcctgt ctttgttccc
      601 gcttgaagcg ttgcttccac ggcgttaatg aattcctggc ggcagtcagg atagcccgag
      661 tagtcgatca cattcgctcc gaaatagatg atcgaggctt ccgatacttc cgcatgagcc
      721 gcagccagag acagaaaaat caggtttcgg cccggcacat aggtagcggg cacatcccgg
      781 ttccgttcgg attcagtccg atgcttcggc accgccatat cgccggtcaa ggccgatcca
      841 ccgatcgccc tcaaatcgac gtccaccact acatgccgct gagcccccag agcggtcgcg
      901 acctgccttg cccgctcaac ctccacagcg tgacgctgct ggtatgcaat ggtgagaaga
      961 tacagcgcgt acccgtcacg ccgcgcaagg gctgccgtaa ccgtggagtc caacccacca
     1021 ctggcaagaa cgaccgctcg ttctgaagtc tgcccattca tgatgtggaa tagaggatgc
     1081 agggtgccgt agggaagaaa tactttggag ttagtcgcgc tcttcttcgc gttcgatttt
     1141 ctccagcaat tccgcgcgcg attggcactc cacacacagc ttggcgaaag ggaccgcctg
     1201 aagacgcttc tcgctaatct cgaccccgca ttcggcgcaa atcccgtaag tcccttcgtg
     1261 tagccgggtg agggcttcgt cgatcgactg ccgtcggcgg ttacgcatct ccatcaagga
     1321 aatgccgagt tcgcgctcaa gatccatcaa ggcttggtca ccgacatcac gcgctgactc
     1381 caaacgccgt tgctgatctt cggtcaagga ctgccccaga ctctcttcga tctccttaat
     1441 aatctcttgg cgtttaccga tgagcatttt gtgaagcact tcctgtcgcc gttcccgtgc
     1501 ttcccgctct tttgccgtct ccttgggccg cacgggcacc tctatttcga cctcgggcgt
     1561 cgctttgagc gctgccgcct cctccgtaat caccggctga ggtttcttga ccgcagtggc
     1621 cgaagatttc gtttttgcct cactcttctt ctttgcctgt gttttcattg tcatagagag
     1681 tccgctccaa aaatggaata agtggttgga gggtcaaaat tccggcttgt atatcacggc
     1741 ccgtgcccct tgaacaagag agcgatggtg aaatgcgcgt cagtcttagg gatagatgat
     1801 ggtcgaatgg acgtcgtagc cggtaagttt gtcgcgtccc ttcaaacttt tcagctcaac
     1861 cagaaaatcg agcccgacaa t
//
LOCUS       DATMTI010000134         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_344701, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000134 DATMTI010000000
VERSION     DATMTI010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344701"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..314)
                     /locus_tag="VN039_02240"
     CDS             complement(117..314)
                     /locus_tag="VN039_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44837.1"
                     /translation="MQHVAVPIMTAVERLSLDTIELAHGARQIGVGGLHDQMKVIVHQ
                     TIGVQQQMFKAMLKRGKKAWG"
     gene            521..1378
                     /locus_tag="VN039_02245"
     CDS             521..1378
                     /locus_tag="VN039_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV44838.1"
                     /translation="MGPTFRPGTVRDAEACGRIGYEAFKAIAEQHGFPPDFPSVDRAV
                     GLLSQILTRPDVYSVVAELDGQVVGSNFLWEGDTIAGVGPITVASTAQNAGLGRKLME
                     SVLDRANARRQVGVRLVQAAYHNRSLSLYSKLGFQAREPLSILQGPPLKLELYGYCIR
                     KATLDDLALCDALCRSVHGHDRRGEIAHAIADGTATVVEHSSRLTGYATGLGFFGHAV
                     GRTNDELTALIGAAPAFTGLGFLLPTRNMELLRWCLNHGLRIVQPMTLMSLGLYQKPA
                     GSFLPSILY"
     gene            complement(1564..1923)
                     /locus_tag="VN039_02250"
     CDS             complement(1564..1923)
                     /locus_tag="VN039_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44839.1"
                     /translation="MGPTKAIVKEHALYEALGGKLIKDGFANRQDLEAYVNHHYLVLP
                     VVDKQGKPWSLDGKPVYCLRGTQYETVNDERVHLARCPDCGGMGIRADEFVVESDCIH
                     CTACGHEFDARLEMMET"
     gene            complement(2064..2243)
                     /locus_tag="VN039_02255"
     CDS             complement(2064..2243)
                     /locus_tag="VN039_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44840.1"
                     /translation="MSWTYWGIVIGLAALVVMLIACMRLLSSNGNEDRQGLRQGAGGS
                     NDIHPEPSIITRRAA"
     gene            2696..3145
                     /locus_tag="VN039_02260"
     CDS             2696..3145
                     /locus_tag="VN039_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44841.1"
                     /translation="MTSVPDKTPGSERLHGVVQVLKAGTIYFLIAVGAEFVLEIIRLQ
                     VVALHVGERLAEMLEIPNVLLATIIGARWVVDRFTLPPLPGIRLSVGLVALILLLIAE
                     GTVILPLHGLSISEYLAVKDAVVGVAPLGALGVLTVMPFLVGYRWER"
     gene            3224..>3695
                     /locus_tag="VN039_02265"
     CDS             3224..>3695
                     /locus_tag="VN039_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV44842.1"
                     /translation="MICPGVFTWGILTLFCNVPTAAIAFEPSDDPESAIRRMVRANAE
                     KDLPTLSQLMAHDADIISYSVAGRKYIGWTELEEGMREEFANSQKLEIPIHELMVWTK
                     GDVAWYAMELDYIRYVAVGPELKRTVFPMRETGVLERRNGSWQLLSWHESFRSAQ"
BASE COUNT          748 a         1050 c         1007 g          890 t
ORIGIN      
        1 tctgtaatcc cggtgatcta gctaggatct agttttttca gccaacacag tatggggaca
       61 atgcggcgtg ccctgcgctg acgaagtggc tgttctgcgg acaacttcct ccccgttcat
      121 ccccaggcct tcttgccccg cttgagcatc gccttgaaca tctgctgctg cacgcctatt
      181 gtctgatgga caatcacttt catctggtcg tggagacccc cgacgccaat ctgtcgagcg
      241 ccatgcgcca gctcaatggt gtctagactt aagcgttcaa ccgccgtcat gatcgggacg
      301 gccacgtgtt gcacggtcgt ttcaaggcga ttgtggtcga cctggagagc tatctgccgg
      361 agctctaccg ctatgtcatg ctcaatccgg tgcgagccag acagcgcgca gctaccttcc
      421 tgtccaatgg ttctccagtt tctcggaaat ccttacgcca tgatctgcca attgactcgt
      481 ctccccgtgc aacggtaact tcattcttaa aggataaccc atggggccga cgttcagacc
      541 aggtacggtg cgggatgccg aggcgtgtgg aagaatcggc tacgaggcat tcaaggcgat
      601 tgccgagcag catggcttcc caccggattt tccatctgtc gatagagcag tgggactgct
      661 ctcacagata ttgacgcgtc ccgatgtgta ttcagttgtc gctgagcttg acggtcaagt
      721 cgtcggcagc aactttctct gggaaggcga taccatcgca ggggtgggac cgatcaccgt
      781 cgcttcgacc gcccagaacg ccggtcttgg acgcaagttg atggaatccg tgctggaccg
      841 tgccaacgct cgccgtcagg tgggggtccg gctggtccag gccgcgtacc ataatcgctc
      901 gctttccctg tactccaaac tcgggttcca ggcgcgagag cctctgtcaa tcctacaggg
      961 accacccctt aagttggagc tgtacggtta ttgtatacgc aaagcgacct tggacgatct
     1021 tgcgttgtgc gatgcattgt gccgtagtgt gcatggacat gatcgccgcg gcgagattgc
     1081 gcatgccatt gccgacggca cagcaacggt cgttgagcat agcagtcggc ttaccggcta
     1141 tgccacgggt cttggtttct ttggccatgc agtcggtcga acgaacgacg aactcacggc
     1201 tctgattggc gctgcgccgg cttttacagg actagggttc ttgctgccga ccaggaacat
     1261 ggaattgttg cgctggtgct tgaatcacgg gctacgaata gtccaaccta tgacgctcat
     1321 gagtctcggg ctctatcaga aacctgccgg ttcctttctc ccttccatct tgtactgatc
     1381 gctctagcag gatgctgaaa aagtctgcta gcggcgttct cgcatcgctt agaggctcaa
     1441 cgtaccgaag cgtacgcctc gccccttcgc tcgctgcggc cttgctgggc agcctttttg
     1501 agcatcctgg tgttattctg gtgttagcac cgaatggcgt cgactgggat ttcccgcagc
     1561 ctgctatgtc tccatcattt ccaacctggc atcgaattca tgtccgcagg ccgtgcagtg
     1621 gatgcagtcc gattcgacga cgaattcgtc ggctcgaatc cccatgcctc cgcaatccgg
     1681 acaacgggcg aggtggaccc tctcatcatt caccgtttcg tattgagttc cacgaaggca
     1741 atagaccggc tttccatcga gtgaccatgg cttcccctgt ttatcgacga ccggcaggac
     1801 gagataatgg tggttcacgt aggcttcaag gtcttgccgg ttcgcaaaac cgtctttgat
     1861 caacttgcct cccaatgcct catagagagc atgctctttc acaatcgcct tcgtcggccc
     1921 cataatatat ctccctccct cttagttaag gttcagactg atcggttggt cacgagcaca
     1981 caacccatcc tttgaattcc accgtctcac cagaaactca ccctcacgct tgtgtccacc
     2041 aggactgcgc cgacaacaaa ccgtcacgcg gctcgcctgg tgatgatcga gggttccgga
     2101 tgtatgtcat tcgatccacc ggcaccttgc cttaatccct gtcggtcctc gttgccattg
     2161 gaagagagca ggcgcatgca cgcaatcagc atcaccacca aggctgcgag accgatgacg
     2221 attccccaat aggtccatga catagatcac ctgctcgcgc atgcgacgcg agtccttcat
     2281 aagattgtcg gcactgatct ctgccgcgaa atcgtatcgc cgtccgtgcg tcatccggct
     2341 tccttagctg caggcagata gcatggaact gccctgccgc gactgtgaca cggtgccaga
     2401 agagggcccg tctggtgagg aacgcacacc cggcaggtcc ggaccgttgg aactggactc
     2461 cacggaggac catgatcacc acctccagaa tgaggcggcc gcgaaagaat aatgtaagcc
     2521 tacgcctcgg acaataccag gtcaagagga agaatctcgt cgttccccta ttcttcagac
     2581 catggatgtg tccgtgattt tccgctagac tgccctgtca gaaatgacca tagctgtctc
     2641 agggcctgca tgaggagaag ccgtaatcga ctgcccaatc ggcgacacat attccatgac
     2701 ttccgtaccg gataagacgc ccggtagtga gagactgcat ggtgtggtgc aggtgttgaa
     2761 ggccggcacc atctatttcc tgatcgcagt tggtgcggag tttgttctcg aaatcattcg
     2821 tctacaagtg gtggccctgc atgtcggcga aaggcttgcc gagatgctgg agattccgaa
     2881 tgtacttctg gcgaccatta tcggagcgcg gtgggtggtt gaccgattta ccttacctcc
     2941 tctgcccggc atccgactga gcgtcggtct tgtggcgctc atcctcttac tcatcgcgga
     3001 aggaacggtt atacttcctc tccatggcct ctcgataagc gagtatctcg ccgtcaagga
     3061 tgccgtggtg ggagtagccc ctctcggtgc gcttggtgta ttgacggtta tgccgtttct
     3121 cgtcgggtat cgttgggaac gctgattcga ttcttcgttc attccctgga ttgatgacct
     3181 acggtgagga aacttgaatt tgttgatgaa aggtaagttc tccatgatct gccccggagt
     3241 atttacgtgg ggtattctga cactgttctg caatgtcccg actgctgcaa ttgcgttcga
     3301 gccctcagat gatccggagt cggccatccg ccggatggtg cgcgcgaacg ccgaaaaaga
     3361 cctgccgacc ctttcacagt tgatggcaca cgatgccgat atcatcagct actcggtcgc
     3421 cggacgtaag tacatcggct ggacagagct tgaggaggga atgcgggagg aattcgccaa
     3481 ttcccagaag ctcgagattc cgatacatga gctcatggtg tggacaaagg gagatgtggc
     3541 ttggtatgcg atggaacttg actatatccg ctatgttgcc gtgggacctg aattgaaacg
     3601 gaccgtgttc ccgatgcggg aaaccggggt tctcgaacgt cgcaacggca gctggcaatt
     3661 attgtcgtgg cacgaatcct ttcgatccgc tcagt
//
LOCUS       DATMTI010000135         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_217789, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000135 DATMTI010000000
VERSION     DATMTI010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217789"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VN039_02270"
     CDS             complement(<1..294)
                     /locus_tag="VN039_02270"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44843.1"
                     /translation="MSSPSVISEDVQHLPPSSFAFVMATGIISIAAHLQGYGVIAWSL
                     FILALVGFGLLWMLTIVRLFRYADEVRANVRNASQAPSFLTSIAGTCILGSQVF"
     gene            complement(681..1046)
                     /locus_tag="VN039_02275"
     CDS             complement(681..1046)
                     /locus_tag="VN039_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWV44844.1"
                     /translation="MLDAPINPLSPLTLSLALCLGLGASASKSGLVAIIGAFLAGMTV
                     AASGQRHTRERQIQSVMPFLVPFFFIETGAQVDIGTLASWSAFGMLVLVTLLAVVSKL
                     GGADWECFHLAETLPLSWE"
     gene            1513..>2378
                     /locus_tag="VN039_02280"
     CDS             1513..>2378
                     /locus_tag="VN039_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWV44845.1"
                     /translation="MFSAETQTQPSGWIVAAAAAVAATIFICDLFAPVGYAVWLLYVL
                     PLWLTSLAPGIRGIPIAIGVVLGLLWGEVALSPGKMTALVISNRALASVLLCIIGWLV
                     FNRKRLAEQREAAQAALRLSEERFRALVQASSDVVYRISSDWTEMRHLVGRDFIDDTL
                     EPSQTWLQKYIPLGDQKHLMATINEAIRLKSVFELEHRVLRMDGTLGWTFSRAIPILD
                     AAGKITEWFGMASDVTARKQAEETVRASDERLQLAMEIGQMAAWDWNISENTVMYSPT
                     LGPLFGLAAGTQ"
BASE COUNT          558 a          640 c          653 g          527 t
ORIGIN      
        1 gaacacctga ctgcccagga tgcacgtgcc ggctatagaa gtcaaaaaac tgggggcttg
       61 ggacgcattc ctgacattgg ctcgcacctc atccgcgtat cggaagaggc gtacgatagt
      121 gagcatccat aataagccaa acccgacaag ggccaggata aacagtgacc acgcaatcac
      181 accatatcct tgcaggtgcg ccgcaatcga aatgatgccc gtcgccatca cgaacgcaaa
      241 ggagctaggc gggagatgct gtacatcttc ggaaatgacc gatggagacg acatggctac
      301 cttctttcgc aattggggat caccgccttc acacggccca attcatgagg actgacggca
      361 tgcggaggcg cgatgtcgag taagtgcaca ccgcttcagg ctgctcctgc ggtctatcgc
      421 catcagcaat ccatacgcga aactgttagc gttgctttct cgcatctggt gttgttcttc
      481 tctcgtttta tcttgaagat agcgttttac gcgtcttctt gaggtgtcga gccagcgagt
      541 aatatcttcc gcagggggag gcgcaataac cgatgtcagc aatgacatgg tcatgatgat
      601 tgcgtaagtt gtatccgaga acactgcatg ctgttttccc agactagcca cgataatacc
      661 aaccccacgc ggcacgattc ctattcccac gataagggca gagtttctgc caagtgaaag
      721 cactcccaat ccgcaccccc caatttgctc accactgcca gcaacgtgac gagaacgagc
      781 atgccaaaag cagaccagct tgccaacgtg ccgatgtcaa cttgcgcgcc ggtctcgatg
      841 aaaaagaatg gcacgaggaa cggcatgacg gattgaattt gcctttcccg cgtatggcgc
      901 tgcccgcttg cagcaaccgt cataccagct aagaatgcac cgattatggc gaccaatcca
      961 cttttggacg cagaggcccc cagtcccaag cacagcgcga gagagagcgt aaggggcgag
     1021 agtggattaa taggtgcatc gagcaaggcc aattgcttta catgattgtt gtgccatcag
     1081 tgtaatgacg atcacaaaac ccactgcctg aatcaaaacg agaccgagtt aggcgaaatc
     1141 cacatcgccg ttctacaagg aaaccttaag ctctgacaaa ggttgtgtca caccctctgt
     1201 cgcttttggt acctttgagt ttaatcgttg gtgcaaaagg ggttatcacg aataggaagc
     1261 gatccggcgc agcaaaaggg cggattgacc cccaagttcc atgcgacatg cgatgcatga
     1321 ggaatcccac gctacgagaa acgtgccgtg aacttcctcg atgcgatcta tcttgcggcc
     1381 ttaatcacct ggcttaattg aagacacacc gtagtataat ggcatttgtt ttaggtatag
     1441 aatacacata ctctgctgca aggccaggaa cccactcgtt acggtgcggc cctttggtaa
     1501 ggagcctcgt acgtgttctc cgcggagact cagacacagc cgagtggctg gatcgttgca
     1561 gcagcagcgg cagtagcggc aacaatcttc atctgtgatc tattcgcgcc agttggctat
     1621 gcggtgtggc tgctctacgt gctgccgctg tggctcacat cccttgcacc gggcattcgg
     1681 ggtattccca tagcgatcgg cgtggtattg gggctgcttt ggggagaggt cgccttgtct
     1741 cccggcaaga tgaccgcgct tgttatcagc aatcgcgctt tggcctcggt gctcctctgt
     1801 atcatcggtt ggctcgtatt caatcggaag cgcctggcgg agcaacgaga ggccgctcaa
     1861 gcggccctgc gcttgagcga agagcgcttc cgtgctctgg ttcaagccag ttcggatgtc
     1921 gtgtatcgca taagttccga ttggacggag atgcgtcatc tcgtgggccg tgatttcatc
     1981 gacgatacat tagagccgag ccaaacttgg ctgcagaaat acattcccct cggcgaccag
     2041 aagcacctga tggctaccat caatgaggcc attcgcctca agagtgtgtt tgagcttgag
     2101 catcgggtgc tacggatgga tggcaccctc gggtggacct tctcacgcgc gattccaatc
     2161 ctcgacgcag cgggcaagat taccgaatgg ttcgggatgg ccagcgatgt gaccgcccgc
     2221 aagcaggccg aggaaacggt gcgcgcgagc gacgagcgtc ttcagttagc catggaaatt
     2281 gggcagatgg ctgcctggga ttggaacatt tcggagaata cggtcatgta ttctcctacg
     2341 ctgggacctt tgttcggttt ggctgccggg acacagca
//
LOCUS       DATMTI010000136         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_344743, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000136 DATMTI010000000
VERSION     DATMTI010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344743"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..714
                     /locus_tag="VN039_02285"
     CDS             355..714
                     /locus_tag="VN039_02285"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV44846.1"
                     /translation="MVVRKFPRFPVAIPSTLVQRDKLRYNASVKDLSLKGCRLESVVR
                     PFTGMQVEVFIELPGEPTPIHISRGAVRWSGSLGIGVEFLTVAPAEQERLKRVVEQLS
                     VKVGQALIVPKDELPKT"
     gene            complement(775..1317)
                     /locus_tag="VN039_02290"
     CDS             complement(775..1317)
                     /locus_tag="VN039_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWV44847.1"
                     /translation="MNYCSHCGAPVTHKIPLGDTLPRHVCDQCERIHYHNPKIVAGCI
                     PEWEDQILLCRRAIEPRLGLWTFPAGFMELGESTEQAAVRETLEEAHADVTITGLYAV
                     LSLPNIGQVYLVFRGRMKTPAFQAGAESLEVRLFPLSDIPWKTMAFQVVSEALRRYVA
                     DAKTGEFRLHLGNLPDKPGF"
     gene            1629..>3739
                     /gene="lon"
                     /locus_tag="VN039_02295"
     CDS             1629..>3739
                     /gene="lon"
                     /locus_tag="VN039_02295"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWV44848.1"
                     /translation="MENNNVLSTLPILPVKRTVLFPGVMMPLTVGRERSIAAVNAAMK
                     TEEKTIVVVAQRDPQAEEPALAELYSIGTKAIVKQIGQSSEGTIHALVQGLDRVVLLK
                     EEQSTPYSVARVRSLGRPTDDGPEVQALHRAIQELVSDLPRLIQAPGIQEVGTVLSNE
                     DDSVALAYRIASLVNLDVVEEQRLLESSSTLDLLRSLYAALSKEIQILQLREKIAKDA
                     QTKIGKNQREYILREQLKAIQDELGEGEGEENEVARLKKQIAEADLPEPIRKEVEREA
                     ARLSKVPPTSPDHQVLRTYLELVLELPWKKASEEHLNLSTVRQVLEEDHYGIKEVKER
                     IVEHLAVLKLNPTAKAPILCLVGPPGVGKTSLGQSIARAMGRTFERFSLGGVHDEAEL
                     RGHRRTYVGALPGRIIQAIRRAGVNNPVLMLDEVDKMGRDFRGDPASALLEILDPAQN
                     HTFRDHYLDLPFDLSKVFFITTANTLDTISQPLLDRMEVIRLQGYSEREKAEIARRYL
                     WPRRLKEAGIEGSQAVLTDEVLNLVISRYTREAGVRQLEQMLGRLTRKVALTFADLPE
                     GQERQPIAIQPDMLGEWLGSERFMPEEARKNLPPGVATGLAWTPTGGDVLYIETTLLP
                     GSHELALTGQLGDVMQESARAARSYLWSHAESMGLDISRFKRNGLHIHVPSGAIPKDG
                     PSAGITMATALASAYEGRAVR"
BASE COUNT          858 a         1054 c         1043 g          784 t
ORIGIN      
        1 ccacgagcat tgtcccgatc ccatattctt atccttactc ctatggctat cctctcggcc
       61 tcggactcgg gtttggactc caatttggcg gcgcgtgaat ttatgcttcg cctttttacc
      121 gtccccattg gggccaccga cattatggtt attggggcta tccttggtga tgttccagat
      181 gaagaaacca acaaccatcc gttagttctg aactctgccc ggcccctgcc cagatcggtc
      241 ttttcgttga caagttgcct ccccccacat agcctgctac ctagtacctc attagtacaa
      301 cggcggtcgg ctcagcaggc tgagaaccca agtcccacaa cacaaggatg cacgatggtt
      361 gttcggaaat tcccccggtt tccggttgca ataccgagta cgttggttca gagagacaaa
      421 cttcgataca acgcttctgt gaaggatctc tcgctgaaag gctgccggtt agaaagcgtc
      481 gtccggccct ttaccggcat gcaagtggaa gtattcattg aactgcccgg tgaacctaca
      541 ccaattcaca ttagcagagg agctgtccgc tggtccggat cactgggcat tggtgtcgaa
      601 ttcttaaccg tggcacccgc agaacaagaa cgactgaaga gggtcgtcga gcaactgtcg
      661 gtgaaggtgg gacaggctct catcgttcca aaagacgagc tgccgaagac gtgaatcgaa
      721 ttcaaggttg aacctctcgc cattccatcg catcaatcgt tcaagttgcc ctatctagaa
      781 cccaggcttg tccggcaagt tgccgaggtg gagtcggaat tcccccgttt tcgcgtcggc
      841 cacgtagcgt cttagggcct cgctgaccac ttgaaaagcc atcgtcttcc agggaatatc
      901 cgacagagga aatagtcgga cctccaaact ttccgctccc gcctgaaatg ccggcgtctt
      961 cattcgacca cgaaatacca agtagacctg tccgatattg ggtagactca gtacggcata
     1021 gagtccggta atggtgacat ctgcatgggc ctcttccaga gtttcgcgca ccgctgcttg
     1081 ttccgtactt tcaccgagtt ccatgaatcc cgcagggaac gtccataggc cgagacgagg
     1141 ttcgatagcc cgacgacaca agaggatttg atcttcccat tccggaatac agcctgcgac
     1201 gattttggga ttgtgataat gaatgcgctc gcactgatcg cacacgtgcc tgggcagagt
     1261 atccccgagc ggaattttat gcgtgaccgg tgcgccacag tggctgcagt agttcatgag
     1321 aacatgcatc gagtccggac acgaacgact cacgacccaa acaccgagca tggatagcac
     1381 aaacgcgcgt tgctttgcac gccggcgtca agcctcccga gcgatgactg cgaaaatgtt
     1441 cggcatggca ggaaccggtg acgaaacctg gtcgaataag agaccttcaa gatcactggc
     1501 atggagcaag acaaacctcc tccccccggc gattattgcc catcccagat ccttcccttg
     1561 acttctacgc aggggaacct atcaggttgt tataatatat cgtgaaccct gaggaaggat
     1621 ggacttcgat ggaaaataat aatgtgttgt caacgttgcc gatactcccg gtcaaacgga
     1681 cagtgttgtt tccaggggtg atgatgccgc tgacggtcgg gcgggagcgg tctatcgctg
     1741 ccgtcaatgc cgcaatgaag accgaagaga agacgatcgt cgtggtcgca cagcgcgatc
     1801 cccaggccga agaaccggca ctcgccgagt tgtattccat cggcacgaag gcgatcgtca
     1861 agcaaatcgg ccagtcgtcc gagggaacga tccatgcgct ggttcaaggg ctggatcgcg
     1921 tcgtcctcct gaaagaagaa cagtccaccc cctactctgt cgcgcgtgtt cgttctttgg
     1981 ggcgtcctac ggacgatgga ccggaagtcc aagcgcttca tcgagccatt caagaacttg
     2041 tgtccgacct accgagactg atccaggctc ccggcatcca agaagtcggc acggtgctga
     2101 gcaacgagga tgattccgtg gcgctggcct accgcatcgc ttcgctcgtc aatcttgacg
     2161 tggtggaaga acagcgcttg ctcgaaagtt cttcgacgtt ggaccttctt cgcagcctct
     2221 acgccgcgct gtcgaaggaa attcaaatac tgcagttgcg ggaaaagatc gccaaggatg
     2281 cgcagaccaa aatcggcaag aaccagcgag agtatatctt gcgcgaacaa ctgaaagcca
     2341 tccaggacga gcttggcgaa ggcgaaggcg aggagaacga ggtcgctcgg ttgaaaaagc
     2401 agatcgctga agccgatttg cccgagccta ttcgcaagga agtcgaacgc gaggccgctc
     2461 ggctgagcaa ggtaccgccg acatcaccgg accatcaggt cttgaggacg taccttgagc
     2521 tggttctcga actcccgtgg aagaaggcat cagaggaaca tctcaatctt tcaacggtcc
     2581 ggcaggtgtt ggaagaagac cactacggga tcaaggaagt gaaagaacgt atcgtcgaac
     2641 atctggcggt cttgaagctc aacccgacgg cgaaagcccc cattctctgc ctcgtcggtc
     2701 ctcccggcgt cggcaaaaca agcttggggc aatcgattgc aagggccatg ggccgaacat
     2761 tcgaacgatt cagcctcggc ggcgtccatg atgaagccga actgcgcggc catcgtcgta
     2821 cctatgtcgg tgcattacca ggccgcatta ttcaggctat ccgtcgggcg ggagtcaata
     2881 atccggtcct gatgctcgat gaagtcgata agatgggacg tgattttcgg ggcgatccgg
     2941 cctcggcgtt gttggaaatt ttggatccgg cacagaacca cacattccgt gatcattatt
     3001 tggatctccc gttcgatctg tcgaaggtct tcttcatcac cacggccaac acccttgata
     3061 ccatcagcca acccttgctg gatcgcatgg aggtgattcg ccttcagggc tacagcgagc
     3121 gcgagaaagc cgaaatcgcc cgacgctatc tgtggccgag acggctcaag gaagcaggca
     3181 tcgaggggag ccaagccgtg ctcacggacg aggtgctgaa tctcgtcatc tcgcgttata
     3241 cccgtgaggc gggtgtgcgc caactcgagc agatgctggg acggttgacc agaaaagtgg
     3301 ccttgacgtt cgccgacctt ccggagggcc aggagcgaca gcccatcgct attcaaccag
     3361 acatgcttgg cgagtggttg ggctccgaac ggtttatgcc ggaagaggcc cgaaagaatc
     3421 tgcctcccgg cgtcgcaacc ggcctggcct ggactccgac aggcggcgat gtgctctata
     3481 tcgaaacaac tttactcccc ggcagccatg agttggccct gacggggcag ttgggcgacg
     3541 tgatgcagga gtccgcgcgc gcagccagga gctatctctg gtcgcatgcc gagagcatgg
     3601 gtttggacat ctctcggttt aaacggaacg ggctccatat tcacgtgcct tccggagcca
     3661 ttccgaaaga cggcccatca gccggcatca cgatggcgac ggcactcgcg tccgcttatg
     3721 aaggcagggc cgtgcggag
//
LOCUS       DATMTI010000137         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_769151, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000137 DATMTI010000000
VERSION     DATMTI010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769151"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VN039_02300"
     CDS             complement(<1..683)
                     /locus_tag="VN039_02300"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HWV44849.1"
                     /translation="MDSLEHVPDMESRRMELRGIVRLAGEVIAQYWPMRTFVHHNPLH
                     SIEYLPFEEAVKRGKQFLGGNGYLPSHLYREYLKAGRIRSTHLDDALKPRIIDKQVSI
                     GSHTVTHGSVLRACLAEGLCAPAMEPLDDQLEDPDRPLIDALARKLELVFLPFSLDDR
                     IRKVVDENQAALCRWLTLSHWCDETLGTQIVQQINEQMIKWCGAFLDEGHATWTMPGR
                     EKGLYEAWKA"
     gene            complement(671..1978)
                     /locus_tag="VN039_02305"
     CDS             complement(671..1978)
                     /locus_tag="VN039_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWV44850.1"
                     /translation="MTGVFIPWLFAGIPFAGALLCLAWWSDPYRVRMSAIACSIISLA
                     VIVGIADRLPTPSDGLLPLYLLPLAAVVAALGQPVHENHRLSWIMTLVFLGLGMGVLT
                     AGNPFGLLCQISLMLIIMVLLYRHHTTLWPISWWGIGSFGAGIAGAGLSLAADPPISS
                     VASLATCAVLLPLMPFHDGYLTALTRLPGSLPPFIVVLLPVLGLHGLATAMPTMPNEF
                     VAVVSLLALMSSLYGAIKALAQSRVRLLVGYGSVSFFSILWWFVAGAHTATPRAAVLA
                     GAVGLATGGLLVAWQVIRTRYGDDVDPQSISGLAAAMPKYAVLVSLLGLAAMGIPPFG
                     VFAGFMGLMLTMPPSSTIGLFIALTAWLAASWYIMQMVQQLLFGTRRPDLRYADLRHP
                     EFTSLLIVVLALLALGLAPTSLYTSDRNPNKTADMERPFAWTR"
     gene            complement(1975..>2775)
                     /locus_tag="VN039_02310"
     CDS             complement(1975..>2775)
                     /locus_tag="VN039_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWV44851.1"
                     /translation="LVQNDIKKTLGFSTIGQMGYMIMECGLGAFSLAVFHLIAHGLFK
                     ATVFLNCGNVIHKARQEPHFPHSGHEDDEVGFSRLTWSTGFVTTLFIPLLILLVTHGV
                     LRIPLLESQGTVILLFFIWITSSQAILTLTRLRAVASWKVSAMMLLTLLFIVFVYLFA
                     VESFTAFLYPNPEEVASYFKTADLPDWLFDLIVVIAAFMTISGWTYLYMRAHGRTVWM
                     PGWIDGVRIRLYTIFLNRIYADELYQLIGSMTAQLIHRIDKLERGWSR"
BASE COUNT          680 a          778 c          818 g          499 t
ORIGIN      
        1 gccttccagg cttcatacag acccttttca cgtcccggca tggtccatgt ggcatgacct
       61 tcgtccagga aggctccgca ccacttgatc atttgttcgt tgatctgctg gacaatctgc
      121 gtgccaagcg tctcgtcaca ccagtgcgag agcgtgagcc atcggcacag agcggcctgg
      181 ttttcatcca cgacttttct gattcgatca tccagcgaaa aaggtaggaa taccagctcc
      241 agcttacgcg cgagggcatc gatcagcggt ctgtcgggat cttccagttg atcgtcaagc
      301 ggttccatgg ccggagcgca aagcccctca gccagacagg cgcgcaagac ggagccgtgg
      361 gtgaccgtgt gcgatccgat gctcacctgc ttatcgatga ttcgtggctt gagcgcgtca
      421 tcgagatgag tcgatcgaat tcgaccggcc ttgaggtact cacgatacaa atggctggga
      481 agatagccgt tgcctcccag gaactgcttc ccgcgcttga cggcttcttc gaacggcaga
      541 tattcgatac tgtgcaatgg attgtggtga acgaaggtcc gcatcggcca atactgcgca
      601 atgacttccc ccgcaaggcg gacgatcccg cgcaattcca ttcgcctgga ttccatgtca
      661 gggacgtgct ctagcgagtc catgcgaaag gtcgctccat gtcggcggtc ttattcggat
      721 ttcgatccga tgtgtacaag ctggtcgggg ccaagccaag cgccagcaga gccagtacca
      781 cgatcaacag tgaagtgaat tcggggtgcc ggagatcggc atagcgtaaa tccggccgcc
      841 gagtgccgaa gagcagctgt tgcaccatct gcatgatgta ccaggatgcg gcaagccagg
      901 ccgtgagtgc gatgaacagg ccgatcgttg aagagggtgg catcgtgagc atcaatccca
      961 tgaaccccgc gaacactcca aagggcggga ttcccatcgc cgcaagaccc aacagggaaa
     1021 ccagaaccgc atatttcggc atcgccgccg ccaggccgct gatggattgc ggatctacat
     1081 cgtcgccgta tctcgtgcgg atcacctgcc aggccaccaa cagtccgccg gtggcaagac
     1141 cgactgctcc ggccagcacg gccgcgcgtg gtgtcgcggt gtgggctccc gccacgaacc
     1201 accagaggat cgaaaagaaa gaaacactgc cgtaaccgac caagagacgg acacgcgact
     1261 gggccaatgc tttgatcgcg ccatataacg agctcattag cgccaagagg ctcacgaccg
     1321 caacgaattc attcggcatc gtcggcatgg cggtcgccag tccgtggagg ccgagcacgg
     1381 gcaacagcac cacaatgaac ggcggcaagc tgcccggcag tctggtcagc gccgtgagat
     1441 acccgtcatg aaaaggcatg agcggcaaca gaaccgcaca ggttgccaaa gatgctacgg
     1501 atgagatggg cggatcagcc gcgagagaaa gtcctgcccc ggcgataccg gcaccgaagg
     1561 agccgatgcc ccaccaggaa atgggccaca gtgtggtgtg gtgacgatac aggaggacca
     1621 tgatgatcag catgagcgag atctggcaca gcagaccgaa cggattgccg gcggtgagca
     1681 cgcccattcc caaaccgaga aagactaagg tcatgatcca ggagagccga tggttctcat
     1741 gaaccggctg gcccaatgcg gccaccaccg ctgcgagtgg cagcaaatag agcggtaaaa
     1801 gaccatcgga cggagtcgga agccggtcgg caattccgac gatcactgca aggctgatga
     1861 tagagcaggc gatcgccgac atcctcacgc ggtagggatc ggaccaccag gccaggcaga
     1921 ggagcgcgcc tgcaaaggga atcccggcga acagccatgg gatgaatacg cccgtcatcg
     1981 agaccatcct cgttccagct tgtcgatccg atgaatcagt tgcgcagtca tcgagccgat
     2041 cagctgatag agttcatcgg catagatccg attcaggaat atcgtataga gtcgaatccg
     2101 tacgccgtcg atccagcccg gcatccagac cgtccgtccg tgagcacgca tgtacaagta
     2161 agtccagcct gagatcgtca tgaacgcggc tatcacgaca atgaggtcga agagccagtc
     2221 cgggagatcg gcggtcttga aatatgatgc gacctcttca gggttggggt agaggaatgc
     2281 ggtgaacgac tcgacggcaa acagatagac gaatacgatg aagaggagag tcagcagcat
     2341 catggccgac actttccatg aagccacggc acgaagccgt gtgagcgtca aaatcgcttg
     2401 ggatgaggtg atccagatga agaagaggag aatgacggtt ccttgggatt ccaacaatgg
     2461 aatccgcagg actccatgcg tgaccaataa gataagcagc ggaatgaaca gtgtggtcac
     2521 aaatccggtc gaccacgtca agcgcgagaa tcctacttcg tcatcctcgt gaccgctatg
     2581 cgggaaatgc ggctcctgtc tcgccttatg gatgacgttt ccacaattga gaaagaccgt
     2641 ggccttgaag agcccatggg cgatgagatg gaagacggcc agcgagaagg ctcccaagcc
     2701 gcattccatg atcatgtagc ccatctggcc gatcgtcgag aagccaagcg tctttttgat
     2761 atcgttttga accag
//
LOCUS       DATMTI010000138         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_726789, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000138 DATMTI010000000
VERSION     DATMTI010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726789"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VN039_02315"
     CDS             complement(<1..207)
                     /locus_tag="VN039_02315"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV44852.1"
                     /translation="MGPINGKAERRSVMDLKVEDQRATPRVYAQFRARVSGSAQPEGT
                     GIILDLSRSGCRLESPLLMLPGLSL"
     gene            complement(389..808)
                     /locus_tag="VN039_02320"
     CDS             complement(389..808)
                     /locus_tag="VN039_02320"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HWV44853.1"
                     /translation="MVRVLAFALHADEALSFGRGVSTEDEPALWQRDVTGAIDLWIEV
                     GQPDEKILRQACGRSKQVIVYVYGVRSAEVWWANQSSSLDRLKNLAVTLLPMESVRAL
                     AGMANPTMQLQWTIQDGHLWITDGVQTLHLELQRLKS"
     gene            complement(942..1154)
                     /locus_tag="VN039_02325"
     CDS             complement(942..1154)
                     /locus_tag="VN039_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HWV44854.1"
                     /translation="MSHPRDPLHGIILETIVTTLVARHGWAKLGARVPIRCFRHHPTM
                     RSSLTFLRKTPWTRKRVEQMIVAELP"
BASE COUNT          357 a          529 c          496 g          380 t
ORIGIN      
        1 taacgagagg ccaggcaaca tgaggagcgg gctttccagc cggcacccgc tccgtgagag
       61 atcgaggatg atgcccgtcc cttcgggctg tgcggagccc gataccctgg cgcggaattg
      121 cgcataaact cggggcgtag cccgctgatc ctctaccttc agatccatga ctgatcgacg
      181 ctccgccttc ccattaatgg gacccatcac tccccctcag aaagaaacag gggagagggt
      241 acttgttctt gtcccctgat tgatctccag gtaacgtatc gctcggaata ggctcaaccc
      301 aagggcttct gactcgggcc gttctgggga agtgaaaaaa atatcgatcg gagaaagcta
      361 catcactcat gtggactgtt tcttcgcgtc agctcttcaa gcgctgtaat tcaagatgga
      421 gggtttggac accgtcggtg atccagagat gcccgtcttg gatggtccat tgtaattgca
      481 tggtggggtt cgccatcccc gcgagagcac gaacgctctc catcgggagc agcgttacgg
      541 cgagattctt cagccggtcg agcgacgagc tctggttcgc ccaccacact tcggcgctgc
      601 ggactccata gacgtacacg atgacctgtt tagagcggcc gcatgcctgc cggaggatct
      661 tctcgtccgg ctgaccgacc tcgatccaca gatcgattgc gccggtgaca tctcgttgcc
      721 acagagccgg ctcatcttcg gtgctcacgc cgcgaccgaa tgacaaggcc tcatctgcat
      781 gcagcgcgaa ggcgagcact cgtaccatct gcgctcctcg gtctcggacg gatggcgcgc
      841 cagtgtgagt gcatgatcct ggaagtactg gcgatccata tctgcgatct gcagtgcgac
      901 tttatagatg gttgcgttcg aagccatcgt gccagggcgc gttaggggag ttccgccacg
      961 atcatctgtt ccactcgctt gcgcgtccac ggcgtcttgc gcaggaacgt cagactcgac
     1021 cgcatcgtcg ggtgatgacg aaagcaacgg ataggcactc gtgcgccaag tttcgcccat
     1081 ccatgccgcg cgaccaaggt cgtgacaatg gtttccaaga tgatcccgtg caagggatcg
     1141 cggggatgtg acatggagtg cggttggttc atggacgaag tggatctgag tgagtgcaga
     1201 gtaccgattg caagtcggtt tcacaagcat gaagaaaagg ggatggcgct cgggctggaa
     1261 tccacgctgc gaaggcgagg acggccatcg aagtcgtcag gaatgtagcc ggtcaccttt
     1321 ttgccttctc gcgaggtaac agcatggtcc tgaaaactag ctgacgtgga tctgagacag
     1381 tgagcttcca gtgaaatgtt atattgcaag acctgacccc attaccacga tcgacaaccg
     1441 cgtctgtctg tgttcgatcc atgcccgccg cgcctccacc ggtacactca gagctttttt
     1501 taagccagtc gagtgccatc tttaaccgac cgatctcttc gtacaacggc gccgtcacgc
     1561 tggcctcgct ccccccactc ccgcgacgaa acacctccgg cgccccctcc actaactgcc
     1621 gcttccactg cgcaatgacc gttggatgca ccccgtgcgt ggcacccaat gcactgagtg
     1681 tcgcacgccc ttgatcgcgt cgagtgccac ccgtgcctta aacacgtccg catgtgtctt
     1741 ccgtttctgt gccatgtccg tc
//
LOCUS       DATMTI010000139         4508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_259801, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000139 DATMTI010000000
VERSION     DATMTI010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259801"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="pssA"
                     /locus_tag="VN039_02330"
     CDS             complement(<1..543)
                     /gene="pssA"
                     /locus_tag="VN039_02330"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWV44855.1"
                     /translation="MKTAGFRSSFGKEGKRRKAAMHLIPNLFTTGNLFCGVYAILSVF
                     NANYVEAAIAILVAMIFDVLDGKSARLTNSTSQFGLEYDSLSDVVSFGVAPGLLIYSW
                     ALSGQGTFGVAVMFAYVAMGAVRLARFNSTVALSDGKYFTGLAIPAAAGVVASLVVFD
                     HYLLKMGGDVRPIVVLLMTLA"
     gene            complement(540..1190)
                     /locus_tag="VN039_02335"
     CDS             complement(540..1190)
                     /locus_tag="VN039_02335"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HWV44856.1"
                     /translation="MADQAVGVPFAKEGIPFIALPAGVTLLTTVLGWPVVACVGAIAT
                     LFSAWFFRNPARVVPQGPKLVVAPGDGKVIAIEEEFEPRYLKERSVRLTIFLNVFDVH
                     INRMPCDGVVEDVQYQPGLFLVASKPEATLRNEQNSMMIKTHEGIKVLCVQVAGLIAR
                     RIVCWISPRDRAIRGERYGLIRFGSRMDTFLPMDTKLRVAVGERVKGGETILGELP"
     gene            complement(1200..2213)
                     /gene="ilvC"
                     /locus_tag="VN039_02340"
     CDS             complement(1200..2213)
                     /gene="ilvC"
                     /locus_tag="VN039_02340"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWV44857.1"
                     /translation="MKIYYDKDADIQQIRNKTVAVIGYGSQGHAHALNLKESGVSVII
                     GLREGASWKKAEQSGLKVMPVADAVKTSDVVMILAPDEAQAAIYRQEVAPNLKAGSYL
                     AFGHGFNIHFGQIVPPASINVFMVAPKGPGHLVRSEYTKGSGVPCLLAIQQDPSGITR
                     QVGLAYASAIGGGRAGVIETNFREETETDLFGEQAVLCGGLTSLIQAGYETLVEAGYS
                     PEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDVTRGPRVVTEQTKQEMKRI
                     LEEIQTGRFAKEWVLENQANRPVYNALLAKGEAHPIEAVGAKLRGMMPWLKKDQLVDK
                     TKN"
     gene            complement(2264..2782)
                     /gene="ilvN"
                     /locus_tag="VN039_02345"
     CDS             complement(2264..2782)
                     /gene="ilvN"
                     /locus_tag="VN039_02345"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWV44858.1"
                     /translation="MEHIISVTVENKFGVLSRVAGLFSGRGFNIESLSVAPTLDPSMS
                     QMTIVTSGDERIIEQIVKQLNKLIDVIKVVDLNETEFVSRETAIIKVHTKDADRAEAL
                     RIVDIFRANVIDSTPSTYTIEVSGDPKKIEAIINLLQPLGIKDLVRTGRVAVAREPVR
                     PVAMQAKKVARD"
     gene            complement(2878..>4508)
                     /gene="ilvB"
                     /locus_tag="VN039_02350"
     CDS             complement(2878..>4508)
                     /gene="ilvB"
                     /locus_tag="VN039_02350"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HWV44859.1"
                     /translation="EQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSV
                     PVVCFSGQVPTNLIGNDAFQEADNVGLSRPCTKYNFLVKDVNDLAATIKEAFYIATTG
                     RPGPVLVDIPKDVSMAKAEFTYPNSVSIRGYNPTYDGNKWQIKQAAEAIMKAKKPILY
                     VGGGVIFSGASQELLELAEMTQIPVDMTLMGLGAFPGEHLLSLGMLGMHGTYQANMAM
                     HYSDLVIAIGARFDDRVTGKVSEFCPYAKVIHVDNDPTSIRKNIHVDIPIVGDCKTVL
                     RELNQILRATVNGEQKGLRKPWWDQIREWQQAHPLTYHQEKEGPVKPQQVVKRLYELT
                     KDRDPIVSTDVGQHQMWAAQYFKLAKPNRWLTSGGLGTMGFGFPAAMGAQAAFRDRLV
                     LCIAGDGSVQMNMQEMATAVVNKLPVKIIILNNGFHGMVRQWQDLFYEGRYASSYLDT
                     TPDFVKLADAYGAIGLRVKKVGDLDAVLKEALATDKPVIVDVPTYPYENCYPMIPAGG
                     CNHEMILEDPPELKKKQSGAAKVTPEDKDTVLTA"
BASE COUNT         1011 a         1379 c         1081 g         1037 t
ORIGIN      
        1 cgccaaagtc ataagcaata ccacgatcgg tctgacgtcc cctcccattt tgaggaggta
       61 atgatcaaag accaccagtg acgctacgac tccggcagcg gctggtatag ccagaccggt
      121 aaagtacttt ccgtccgaca gtgccacggt cgagttgaat cgcgccaatc gcacggctcc
      181 catggccaca taggcaaaca tcacggccac gccgaacgta ccctgtccgc tcaatgccca
      241 ggagtaaatc aacaaacctg gtgcgactcc gaatgacacc acatcggaca gcgaatcata
      301 ctccagccca aactgactcg tactgttggt cagtctggcc gatttcccgt ccaagacgtc
      361 aaaaatcatc gcgacaagaa tcgcgatggc cgcttccaca tagttggcgt tgaagacgga
      421 caagatcgca tagactccgc aaaataagtt gccggtcgtg aagaggttcg gaataagatg
      481 catggcggcc ttccgcctct tcccttcctt tccaaatgaa cttctgaaac ccgccgtttt
      541 catggcaact ctcccagaat ggtttcacca cccttcaccc gctcaccaac agccacccgg
      601 agctttgtgt ccatcggaag aaaagtatcc atgcgcgatc cgaaacggat caatccatat
      661 cgttcacctc gaatggctcg atcccgtggc gagatccaac acacaatgcg tcgggcgatc
      721 aaccctgcta cttgcacgca cagaactttg atgccttcat gcgtcttgat catcatgctg
      781 ttctgttcgt tccgcaacgt cgcttccggc ttgctggcga ccaaaaacag ccccggttga
      841 tattgcacat cttcgaccac cccgtcacat ggcatcctat tgatgtgcac atcgaagaca
      901 ttcaaaaaaa tcgtaagtct gacactgcgc tctttgaggt agcgcggctc aaactcttcc
      961 tcgatggcga tcactttccc atcgccggga gcgaccacaa gctttgggcc ttgcggcacg
     1021 acccgagccg gattccgaaa aaaccaggca gaaaacaacg tcgcgatagc gccgacacat
     1081 gcgacgaccg gccagcccag cactgtagtc aacagtgtaa cgccggctgg aagcgcgatg
     1141 aagggaattc cttctttagc aaagggaacg ccgaccgctt gatccgccac ggagttgcct
     1201 cagtttttag ttttgtcgac gagctgatcc ttcttgagcc acggcatcat cccgcgcagc
     1261 ttggctccta ccgcctcgat aggatgcgcc tcgcccttag caagcaacgc attatagacc
     1321 ggtcgattgg cttgattttc caagacccat tctttggcga accgtcctgt ctgaatctct
     1381 tcgagaatcc gcttcatctc ttgcttcgtc tgttcggtca ccacacgcgg acctctggtc
     1441 acatcaccgt attttgctgt cgtgctgatc gaatagcgca tgttggcgat cccaccctga
     1501 taaatcagat cgacgatgag cttcacctca tgcaaacatt cgaagtaggc catctccggc
     1561 gagtacccgg cttcgaccaa ggtctcatac cctgcctgga tcagggacgt cagccctccg
     1621 cagagcacgg cctgttcgcc gaagagatcg gtctcggttt cttcgcgaaa attcgtctcg
     1681 atgacaccgg ctcgtccacc gccgatcgca ctcgcgtagg ccaatccgac ctgtctcgtg
     1741 ataccgctgg gatcctgctg gatcgccaga aggcaaggca ccccgctgcc cttcgtatac
     1801 tcggaacgaa cgagatgtcc cggtccctta ggggcgacca tgaagacatt gatggaggcc
     1861 ggcggcacta tctgcccaaa gtgaatattg aacccatgac caaatgccaa ataggatcca
     1921 gccttaagat tgggcgccac ttcttggcga taaatggcag cttgcgcttc atcgggcgcc
     1981 agaatcatca ccacatcgga agtcttcacg gcatccgcga cgggcatgac tttgagtccg
     2041 ctctgctcgg cttttttcca cgaggcaccc tcacgcaacc caatgatgac cgacacaccg
     2101 ctttctttca ggttcagcgc atgcgcatgg ccctgacttc catagccgat cacggccacg
     2161 gtcttgtttc gaatctgctg aatatcggca tccttatcat agtaaatttt catgacccac
     2221 cctttccctc agtatatagc aaaatttggc gtgtagcagg acgttagtcg cgggcgactt
     2281 tcttggcttg catcgcaact ggacgaaccg gttcccgcgc gacggccacc cggccggtgc
     2341 gcaccaaatc cttgattcca agaggctgca acaagttgat gatcgcttca atcttcttgg
     2401 ggtctcccga aacttcgatg gtgtaggtgc tcggcgtcga atcgatcacg ttcgcccgaa
     2461 agatatccac gattctgagc gcctcggctc gatccgcgtc cttcgtatgc accttgatga
     2521 tcgccgtctc tcgtgaaaca aactccgttt cgttcaggtc cacgaccttg atgacatcga
     2581 tgagtttatt cagctgcttc acgatctgtt cgataatccg ttcgtcaccg gacgtcacaa
     2641 tcgtcatctg agacatggat ggatcaagcg tcggagcgac cgacaggctc tcgatattga
     2701 acccacggcc gctgaacaac cccgcgactc gcgacaagac tccaaactta ttttcaacag
     2761 tcaccgagat gatatgttcc attgaatgca aagagcttat ggctgatggc gtatggcaaa
     2821 aaccaacgaa ccaatttcag gctcgtgcca tcggctatac gccatatgct cttactccta
     2881 tgccgttaaa accgtatctt tatcctccgg cgtcaccttt gccgcaccgg attgtttctt
     2941 cttcaactca ggcggatcct ccaagatcat ttcatggttg cagccgccgg ccggaatcat
     3001 cggatagcaa ttctcatagg gatatgtcgg cacatccacg atgacgggtt tgtccgtcgc
     3061 caaggcttct ttcaggacgg catcaaggtc gccgaccttc ttgactcgca agccgatcgc
     3121 tccataggca tccgctagtt tgacgaaatc cggagtggta tccagatagc tcgacgcgta
     3181 acgtccttcg tagaatagat cctgccattg tcggaccatg ccgtgaaatc cgttgttcaa
     3241 gatgatgatc tttaccggca gcttattgac gacagctgta gccatttctt gcatgttcat
     3301 ctggacactg ccgtccccgg cgatacagag caccagccga tcacggaatg cagcctgtgc
     3361 acccatcgca gcggggaatc caaaacccat cgtgccaagc ccacctgacg tcaaccacct
     3421 gttcggcttc gccaacttga aatactgcgc ggcccacatt tggtgctgcc cgacatccgt
     3481 tgagacgatc ggatcccggt ccttggtcaa ttcatacagc cgcttgacca cctgctgcgg
     3541 cttgaccggc ccctcctttt cttggtgata ggtcaacggg tgggcctgct gccattctcg
     3601 gatctgatcc caccagggtt tccggaggcc cttctgctct ccattgaccg tcgcccgcaa
     3661 gatctgattc aactcacgga gcaccgtttt gcagtcaccg acgatcggaa tgtcgacatg
     3721 gatgtttttc cgaatggagg tcggatcgtt atcgacatga atcactttcg cataaggaca
     3781 gaactccgac acctttcccg tcacccgatc gtcaaaccgt gcgccgatcg cgatcaccag
     3841 atcggaataa tgcatagcca tgttagcctg ataggtcccg tgcatcccga gcatcccaag
     3901 ggagagaaga tgctctccag gaaacgcgcc aaggcccatc aatgtcatgt ccaccgggat
     3961 ctgtgtcatc tcggccagtt ccagcagttc ctgtgaggct cccgagaaga tcaccccgcc
     4021 gccgacatag agaatcggct tcttggcctt catgatcgct tcggctgcct gtttgatctg
     4081 ccatttattc ccgtcatagg tcgggttgta cccacgaatc gagacagaat tcggataggt
     4141 gaactctgct ttggccatcg acacatcttt cgggatatcc accaggaccg gacccggacg
     4201 accggtcgtc gcgatataaa acgcctcttt gatcgttgcc gccaagtcgt tcacgtcctt
     4261 gacgagaaaa ttgtacttcg tgcatggtcg gctcaaaccc acgttgtcgg cttcctgaaa
     4321 ggcgtcgttc ccgatcaaat tggtcgggac ttggccgctg aaacagacta ccgggacaga
     4381 atccatataa gcatcggcta gtgccgtaat aacgttggtc atgccggggc cggaggtgac
     4441 cagacacacg ccggccttgc cggtcgcctt cgcatatcct tccgccatat ggcccgcgcc
     4501 ctgttcat
//
LOCUS       DATMTI010000140         1794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_218057, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000140 DATMTI010000000
VERSION     DATMTI010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218057"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..615)
                     /gene="pgsA"
                     /locus_tag="VN039_02355"
     CDS             complement(19..615)
                     /gene="pgsA"
                     /locus_tag="VN039_02355"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWV44860.1"
                     /translation="MNRVLEVWRDIGQDSLNLPNLLTLVRILLIPVFIILFINPTPDQ
                     SLAAAIIFTVAAVTDMLDGYIARRTGQVTKLGKLLDPIADKLLVLSALILLMNVDRVS
                     AMVALLIIAREVAVTGIRAIAASEGMIIPAETTGKYKMALQVIAIILLILEGTGLAEL
                     GNMHLAGTVTLYLSLVLGYLSGSQYVWTFWKQVVAKGL"
     gene            complement(631..1638)
                     /locus_tag="VN039_02360"
     CDS             complement(631..1638)
                     /locus_tag="VN039_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HWV44861.1"
                     /translation="MTVMPSGRESKAAKMEESLADGRRVLEIEARAVQSLVGRLDAKF
                     AKAVDLLVQCKGKVVVSGMGKSGLIGQKIAATLASTGTSSFFLHPAEGVHGDLGMLAR
                     RDALVAISNSGETQELLQLLPYVKRLGIPVIAFTGRMTSTLAKNVDVALDVSVQEEAC
                     PMGLAPTASTTATLALGDALAVALLQKREFKEEDFARFHPGGTLGRRLLIKVKDLMHA
                     DSEIPMVQAAVGGMAAMLEMSAKKLGMTTVVDQEGELVGVITDGDLRRFIQRGTDLVN
                     TTAGELASHNPKTIGPHELAATAVEMMERFSITTLVVTEDGRAIRGVIHLHDLLKNGI
                     V"
BASE COUNT          368 a          543 c          478 g          405 t
ORIGIN      
        1 ctagtttcaa catcctgact acaatccctt cgcgacgacc tgcttccaga aggtccatac
       61 atactggctg ccggagagat aacccagcac cagcgacaaa tacaatgtca cagtgccggc
      121 caggtgcata ttgccgagtt ccgcaagccc cgttccttcc aggatcagca ggatgatggc
      181 gatgacttgg agtgccatct tgtatttccc ggtcgtttcc gccgggatga tcatcccttc
      241 gctcgcagca atggcgcgga tgcccgtgac cgccacttcc cgtgcgatga tcagtagtgc
      301 gaccatcgcg ctgacccgat ccacattcat gaggagaatc aatgccgaca agaccaggag
      361 cttatccgcg atcggatcga gtagtttgcc gagcttcgtc acctggccgg ttcgccgggc
      421 gatgtagccg tccaacatat ccgtcaccgc tgcgacggta aagataatgg ctgcggccag
      481 cgattgatcg ggcgttgggt tgatgaaaag aatgataaag acgggaatca agagaatgcg
      541 gacgagtgtc aggagattgg gcagattgag cgagtcctgc ccgatgtctc gccacacttc
      601 cagaacgcgg ttcatggcta cacctgtctc ctaaacaatc ccgttcttga gcagatcgtg
      661 tagatggatg actcctcgga tagcccgccc atcttccgtc accaccaggg tcgtgattga
      721 aaatcgctcc atcatctcca ccgccgttgc cgccaattcg tgcggtccga tcgttttggg
      781 attgtgcgag gccaactcgc ctgccgttgt gttcaccaga tccgtgcccc gctggatgaa
      841 gcgtcgcaag tcgccgtcgg taatgacgcc gaccaactca ccctcttgat cgactacggt
      901 cgtcatgccg agtttttttg cgctcatttc aagcatggcc gccatacccc cgacggcggc
      961 ttggaccatc gggatctccg aatccgcatg catgagatct tttaccttga tcagcaaccg
     1021 gcgtcccaag gtgccgccgg gatggaaccg ggcaaaatcc tcttctttga actcccgctt
     1081 ttgtaataaa gcaacggcca atgcatcacc taatgccaac gtggcggttg tgctggcggt
     1141 cggagccagc cccattgggc aggcctcttc ctgtaccgac acgtcgagcg cgacatcgac
     1201 atttttagcc agggtcgaag tcatccgtcc cgtgaacgca atcaccggaa tgcccaaacg
     1261 tttcacgtac ggaagcaatt gaagcaactc ttgcgtctcg ccgctgttgg aaatcgcgac
     1321 cagcgcatcc cggcgagcca acatgcccag atctccgtgg acgccttcag cgggatgcag
     1381 gaagaacgac gacgtaccgg tgctggcgag cgtcgcggca attttctgcc cgatcaggcc
     1441 ggacttgccc atgcccgaca cgacgacctt tcccttgcat tgaacgagca gatccacggc
     1501 tttggcaaac ttggcatcca gccggcccac gagcgattga acggctcgag cctcgatttc
     1561 gaggacacgc cgtccgtcag cgaggctttc ctccattttc gcggctttcg attctcggcc
     1621 ggatggcatc acagtcatgg gtttgctgcg tctcgtcaca gcctttgata ccggactcgc
     1681 taccttccct ctcatggttg tgttgcgttg cgcggatggt ttcagcttct tggctttgct
     1741 gcgtcgcata tccgtatgac ccgttcaagt agggatttca attgatgcaa cggt
//
LOCUS       DATMTI010000141         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_769282, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000141 DATMTI010000000
VERSION     DATMTI010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769282"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1546
                     /locus_tag="VN039_02365"
     CDS             <1..>1546
                     /locus_tag="VN039_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HWV44862.1"
                     /translation="KPVVISTATIALQEQLMEKDIPFLSKAAGLDNVNVKLVKGRNNY
                     LCIQKLTEVEGQIRPNSSEMLHIQYLKAELQRGWDGDLANLELAIPAELWKEVRSDSE
                     DCLGRRCRFYDRNPYRKAREDLDQADILISNHALYLQDLSAAQSLLPPHDVVVFDEAH
                     QLKHWALNAFTIRIGKFATQKLLQKIHRRLQPLPEHFHHLIADSEAAIFEWLFRTEQG
                     AFRLHADDLFLYVVNKHIQVLKDLELWLTGMDVKQLSVLRPDMIQSELDLDKAKVQRE
                     KLVSQLQGLIMRWEYFLEEDPFSKQRVNWAEVDRERLYYELKSTPLNIAEMMGLQLWQ
                     QKTAVLTSATLSVNNNLNFTRNDLGIGDEASDLVLPSPFRYEDQCVLYLPEHLPDPNS
                     AEFTSAITEEIAEILAVSQGRAFVLFTSNSAMFRVSNALIPRLPFPCKVQGEMPRNRL
                     IDWFKTTPNSVIFATSTFWEGIDVPGEALSCVIMDKIPFSPPNDPVNQAVVDFIKSKG
                     DDWFGS"
BASE COUNT          358 a          472 c          406 g          310 t
ORIGIN      
        1 agaagcccgt ggtcatttcc accgcgacga ttgccctgca agagcagttg atggagaagg
       61 acattccctt cctctccaag gcagcagggt tggataatgt caatgtcaag ctggttaagg
      121 gtcgcaacaa ctacctctgc atccagaaac tcaccgaagt ggaagggcaa attcgcccca
      181 actccagcga gatgctgcat attcaatacc tgaaggccga actgcaacgc ggctgggatg
      241 gggatttggc gaatttagaa ttagcgattc ccgctgaatt atggaaagaa gtacgctccg
      301 attccgagga ttgcctgggc cgccggtgcc gcttttacga ccgaaacccc taccgcaagg
      361 cgcgggagga tctggatcaa gcggatattc taatctccaa ccacgcctta tacttgcagg
      421 atttatccgc cgcacaaagc ctgttgccgc cgcatgacgt ggtagtcttt gatgaggccc
      481 accagttgaa acactgggca ctgaacgcct tcaccattcg catcggaaag tttgccacgc
      541 agaagctatt acagaagatc catcggcgct tacagccctt gccggagcac ttccaccacc
      601 tcatcgccga ttccgaggcg gccatcttcg agtggctgtt ccgcaccgag caaggcgcct
      661 tccggcttca cgcggacgat ctgttcctct acgtggtcaa caagcacatt caggtgctga
      721 aagatctgga actctggctg accgggatgg acgtgaagca gctctccgtc ctgcggcccg
      781 acatgatcca gtccgagctc gatctcgaca aggccaaagt ccagcgggaa aaactggtca
      841 gccagttgca aggcttaatc atgcgctggg aatacttcct ggaggaagac cccttctcca
      901 aacagcgggt gaactgggcg gaagtcgatc gggaacgcct ctattacgaa ctcaaatcca
      961 ccccgctgaa cattgccgag atgatgggtc tgcaactctg gcagcagaag acggcggtgc
     1021 tcaccagcgc cacactctcg gtgaacaaca atttgaactt cacccgcaac gatctgggca
     1081 tcggggacga ggccagcgat ctggtgctac ccagcccctt tcgctacgaa gaccagtgcg
     1141 tcctttacct gccggaacac ttgccggacc ccaacagcgc cgaattcacc agcgccatca
     1201 cggaagagat cgccgagatc cttgcagtca gccaagggcg ggcctttgtg ctgttcacca
     1261 gcaactcggc catgttccgg gtcagcaacg cgctcatccc gcgcctgccc ttcccctgca
     1321 aagtccaagg cgagatgcct cgtaaccgac taatcgactg gtttaagacc acgcccaaca
     1381 gcgtgatttt cgccacttcc accttctggg agggcattga tgtccccggg gaggcgctca
     1441 gttgcgtgat tatggacaag atcccctttt ccccgcccaa cgacccggtc aaccaggccg
     1501 tggtggactt catcaaatcc aagggcgacg actggtttgg ctccta
//
LOCUS       DATMTI010000142         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_218106, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000142 DATMTI010000000
VERSION     DATMTI010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218106"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="grpE"
                     /locus_tag="VN039_02370"
     CDS             <1..616
                     /gene="grpE"
                     /locus_tag="VN039_02370"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWV44863.1"
                     /translation="YGYLTACIGIRMSNDQEQEKINANTIESLEEDSPVTAAGSSFQE
                     ELAGKTEECKALNDKYLRLAAEFENYKRLTQRDQREQIRFGNEQLLKELLPVVDNMER
                     AIKAARTNSGDSALVQGVELTLKQLSGVLAKFGVQAIETSGQEFDPSAHQAVSYGPSN
                     DVPANKVLDEFQKGYRLHDRILRAAMVSVSSGPAQPSEHGSTTN"
     gene            658..2577
                     /gene="dnaK"
                     /locus_tag="VN039_02375"
     CDS             658..2577
                     /gene="dnaK"
                     /locus_tag="VN039_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWV44864.1"
                     /translation="MGKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRTTPSVVAITD
                     KSERLVGQIAKRQAITNPENTIFSVKRLMGRKFKSREVQEAMKRLPYKVVEADNGDAH
                     VELRGKRYSPPEISAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDISVLEIGEGVFEVKSTNG
                     DTYLGGDDFDLRVMDWLVDEFRKDQGIDLRKDRMALQRLKESAERAKIELSSSQETEI
                     NLPFITADASGPKHMVTKLTRAKLEQLVDDLIQRTIEPCRKALSDAGVTAKDIQEIVL
                     VGGMTRMPKVIQVVKDFFGKEPHRGVNPDEVVAIGAGIQGGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTVPTKKSQVFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGIPPAPRGMPQIEVTFDIDANGIVHVSAKDLATQKEQSIKITASSGLSKDEVE
                     KLVRDAQSHTEEDKKRRKLAEAKNQADTLVYQTEKNITEYGDKVSAEEKTKIQDAVAT
                     VKKALEGTDAEAIESATQTLMTASHKLAEEMYKKAAASPGPQPGASSSDSTGETKTDE
                     KVVDAEFEEVDKDKK"
     gene            2663..>2756
                     /locus_tag="VN039_02380"
     CDS             2663..>2756
                     /locus_tag="VN039_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWV44865.1"
                     /translation="MSKRDYYETLGVDRNVSDDELKKAYRKLARQ"
BASE COUNT          743 a          732 c          781 g          500 t
ORIGIN      
        1 ctatggatat ctcaccgctt gtataggtat caggatgagt aacgatcaag agcaggaaaa
       61 aataaacgcc aatacaatcg agagcttaga ggaagattct cctgtcacgg ctgcgggctc
      121 ctcatttcaa gaggaactgg ctggcaagac tgaagaatgt aaagcgctca atgacaagta
      181 tctccggctg gctgcagaat tcgaaaacta caaacggctg actcaacgcg accagcgtga
      241 acaaattcga ttcgggaatg aacagcttct taaagagctg ttgccggttg tcgacaacat
      301 ggagcgggcg atcaaggctg ctcggacgaa cagtggcgat tcggcgcttg tccagggtgt
      361 ggaactgacg ctcaagcaac tatccggggt cctggccaaa ttcggcgtgc aggcgatcga
      421 aacgtccggc caggagttcg acccaagcgc ccatcaagct gtctcgtatg ggccgtcgaa
      481 cgacgtaccg gccaataaag tattggacga gtttcaaaaa gggtatcggc tgcacgatag
      541 gattctgcgg gcggcaatgg tcagtgtatc gtcaggtccg gcccaaccaa gcgaacatgg
      601 ctcaacgacg aactgacttg attctgtcgt cgttgtcgag aaggaaggag ttcaccaatg
      661 ggtaaagtca tcggtatcga tctcgggacc accaactctt gtgtggcgat tatgagcggc
      721 ggagaccccg tcgtgatcgc caacgccgaa gggagccgaa ccactccttc cgtcgtcgcc
      781 atcaccgata aaagtgaacg gctggtcggc caaatcgcca aacggcaggc gattaccaat
      841 cccgagaaca ccattttctc cgtcaagcgc ctgatggggc gcaagttcaa gagtcgtgag
      901 gttcaagaag ccatgaagcg gcttccctat aaggtcgttg aggccgacaa cggcgatgcg
      961 cacgtggaat tgcgcggcaa gcgctatagc ccgccggaaa tctcggccat gattctgcag
     1021 aaaatgcggc agaccgctga agactacctc ggtgaaaagg tcactgaggc cgtcgtgact
     1081 gtccctgcct attttgatga cagccaacgt caggcgacta aagatgccgg tcagatcgcc
     1141 ggtctgaatg tcctgcgcat catcaatgag ccgacagctg cctccctcgc ctacggtctg
     1201 gataagaaga aggacgaacg gattgccgtg tacgatttgg gcggcgggac tttcgacatc
     1261 tccgtgctgg aaatcggcga aggcgtcttt gaagtaaaat ccaccaatgg tgatacctat
     1321 ctcggcggag acgacttcga tctacgcgtg atggattggc tggtcgacga gtttaggaag
     1381 gatcagggca ttgatttgcg gaaagatcgg atggcgcttc aacgcctgaa ggaatcggcg
     1441 gaacgagcca agatcgagct gtcgtcatct caagaaacgg agatcaatct gccgttcatc
     1501 accgccgatg ccagcggtcc caaacatatg gtcaccaagc tgacgcgagc caagctcgaa
     1561 cagcttgtgg acgacttgat tcagcgcacg atcgagccct gccgaaaagc cttgtccgac
     1621 gcgggtgtca ccgccaagga tatccaagaa atcgtgctgg tcggcggcat gacccgcatg
     1681 cccaaagtga ttcaggtggt gaaagacttc tttgggaaag aaccgcaccg tggagtgaat
     1741 cccgacgaag tcgtcgccat cggagccggc atccaaggcg gagtcctcaa aggagaggtc
     1801 aaggacgtcc tgcttctcga tgtgactcca ttgtcgttgg gtattgagac gttgggcggc
     1861 gtattcacca agctcatcga acgcaacacg accgttccga ccaagaagag ccaagtgttc
     1921 tcgacggcag ccgataacca aacggccgtc accattcgcg tcttccaagg cgaacgggaa
     1981 atggccaatg acaacaaact gctcgggcag ttcgacttgg tcggcattcc accggcgcct
     2041 cgcggtatgc cgcagattga agtgacgttc gatatcgatg ccaacggcat cgtgcacgtg
     2101 tcggctaagg atctggccac acagaaagaa caatccatca agatcacggc ttccagcggc
     2161 ctgagcaagg acgaagtcga gaagcttgtt cgggatgcgc agtcgcatac cgaagaagac
     2221 aagaaacgcc gcaaattggc ggaagccaag aaccaggcgg acactctggt ctatcaaact
     2281 gagaaaaaca tcacggaata cggcgacaaa gtctcagcgg aggaaaagac aaaaatccag
     2341 gatgccgtcg caaccgtcaa aaaagcgctc gagggcaccg atgccgaggc aatcgaatcg
     2401 gcgacacaaa cacttatgac ggcatcgcac aagctagccg aagagatgta caagaaagca
     2461 gccgcatctc cgggtccgca accgggcgcc tcgtcatccg acagcaccgg cgaaacaaag
     2521 accgacgaaa aagtcgtgga cgcagaattt gaagaagtag acaaagacaa aaaatagcct
     2581 agaatagcgg ttcagagtgc ccgagctctc gacgcgatcg gaagctcggg cgggttccct
     2641 acttccatac acaatggcag ccgtgtccaa acgcgattac tacgaaactc tcggcgtcga
     2701 tcggaatgtg tccgacgacg agctgaaaaa agcctaccgg aaactggccc gccagc
//
LOCUS       DATMTI010000143         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_557334, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000143 DATMTI010000000
VERSION     DATMTI010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557334"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2933
                     /locus_tag="VN039_02385"
     CDS             <1..>2933
                     /locus_tag="VN039_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV44866.1"
                     /translation="RILLSGTALCVGLLSTAAVAQSDAVAVGAQADPASQTASGGDSA
                     GNDIVVVGSRIEGSRVTEALPVTVVSKDDITNTAAVSGDELFRSIPQMGDVTFNSQYI
                     PNSSNSARGDVGSVNLRNLGPGNTLVLINGRRLVTHPVSQADENLVPVISYNTNAIPV
                     VGVERLEVLRDGAAAIYGTDAVAGVVNVVLRDDYDGVSFDSQYGFAQGTGLRETSLNS
                     MVGHNFRDGNITLFASYNHRDALNANDQDFTSSLDRRPLFDGTRFEDAASLDGTETTT
                     PWASLQSPQSFGTIYQGSTPLTSSSGLFHIQPDANPGCATSVGEGICLGTGSLATSGA
                     ARNLRFDPRKDYNTTIIPKLDRINLYLTGHYDISPEVTLFGEAGYYYARTRSWQEPRH
                     TLGGVPITVPASNYWNPFGPVTFADGTANPNRLPGTNVPIDGLPVTIRSYSFADLGPM
                     EVVDKNSQYRLLAGIRFPFLGFKWETAGVYSAARVRDTGDGISYTKLQQQLALSTPDA
                     YDPFNGSNLVDYRGADSTSSSQAALDAIRVKVNRIDKSTLAMWDLKGSKSDLLDLPGG
                     PLGIAIGTELRRETQSDNRDPRLDGTIQYTDAVTGHIYPTDVVGESDTPDTKGHRVVA
                     SAYGELAIPLVSEDMHVPLVRHAELQVAGRYEHYSDVGSVAKPKVAGAWDVFDGVRFR
                     GSWAQGFKAPNLEQLNATSVTRTNTRTDWIRCEAETRLPADNPLHIDSFTDCAERFST
                     PEQRAGNKELKPETSETWSVGAVLQPKFIPHDFGRFTFTVDLWHVKQNGIVGVFGGAN
                     ALIEDYYDRLNGASNTNVVRAAPTAEDVALFTAAGMDPVGQILSVKDQYRNLLPQTAS
                     GIDFGFNWQLPNTSVGSFSFDLNVAYLRKFYRSPIPEVAALMAARDAGEINPGTTFSQ
                     AGGDLIGQGSRPKWKGSASLTWSYQQFQVGAFTQYVGSVYDPDLIDDLGEYWSVDSQL
                     TGNLY"
BASE COUNT          652 a          848 c          804 g          629 t
ORIGIN      
        1 gaggattctt ctttcgggaa cggcgctgtg cgtcggattg ttgagcacag cggctgtcgc
       61 ccaatcggac gccgtcgctg tcggggccca ggccgatccg gcgtcgcaaa ccgcttcggg
      121 cggagacagc gcagggaatg acatcgtcgt ggtcggttcc cggatcgaag gaagcagggt
      181 gaccgaggcc ttgccggtca cggtcgtctc aaaggacgac atcaccaata ccgcggcggt
      241 ttccggcgac gaacttttcc gttcgatccc gcaaatgggc gatgtgacgt tcaacagcca
      301 gtatattccg aacagcagca actcagcgcg tggcgacgtc ggatccgtca atctgcgcaa
      361 tcttgggccg ggcaatacgc ttgtcctgat caatgggcgg cgcctcgtga cacacccagt
      421 cagccaggct gacgaaaacc tagttcccgt aatatcctac aacaccaatg cgattcctgt
      481 tgttggcgtg gagcggctgg aagttcttcg cgatggtgcc gctgcgatct acggaaccga
      541 cgccgtcgcc ggcgtggtga atgtcgtcct tcgcgacgac tatgacggtg tcagcttcga
      601 cagccaatat ggcttcgccc aaggcaccgg acttcgagaa acaagtctca actcgatggt
      661 tggccataac tttcgagatg gcaacattac gctattcgcg agttacaatc atcgcgatgc
      721 gctcaatgcc aacgatcagg attttacctc atcgctcgat cgccgccctc tattcgacgg
      781 tacccggttc gaggatgcgg caagcctgga cggcacggaa accacgacgc cttgggctag
      841 tttgcagtcg cctcaaagtt ttggaacgat ctatcaaggc tccacgcccc tcaccagttc
      901 atccggcctt tttcatattc agcccgatgc caaccctggt tgcgcaacct cggtcggcga
      961 aggcatttgc ctcggcaccg gttcgctcgc aacgtcggga gcggcccgaa acctacgttt
     1021 cgatccgcgc aaagactata acacgacgat catccccaag ctcgaccgga tcaaccttta
     1081 ccttaccggt cattatgaca tctctcctga ggtaacgttg ttcggcgaag ccggctatta
     1141 ttacgccagg acgcggagct ggcaggagcc gcgacacacg cttggcggtg tcccaattac
     1201 agtaccggct tcgaattact ggaatccgtt tggccccgtc actttcgccg atggcacggc
     1261 caaccccaac cgcttgccag gaaccaatgt tcccatagat ggacttcctg taaccatccg
     1321 ttcctatagc tttgccgatc ttggtcccat ggaagtcgtc gacaaaaaca gccagtatcg
     1381 acttctcgca ggaatccgct ttccatttct tggctttaaa tgggagacgg ctggtgtcta
     1441 ttctgctgcg cgtgttcgcg ataccggaga cggtatcagc tataccaagc tgcaacagca
     1501 actcgctctc tctacccccg acgcctatga tccattcaac ggctcaaatc tggttgacta
     1561 tcgcggtgcc gacagcacgt cgagcagcca agcggcgctt gatgctatcc gggtgaaggt
     1621 caatcggatc gataaatcca cgctggcaat gtgggaccta aagggcagca agtccgacct
     1681 gctcgatctc cccggaggcc ccctcggaat cgccatcggt acggaattgc gccgagaaac
     1741 ccaatcggac aatcgagatc caagattgga tggcacgatt cagtatacgg atgcggtaac
     1801 cgggcacatt tatccgactg atgtggtcgg agaaagtgac acgccggata ccaaaggaca
     1861 ccgcgttgtc gcttctgcgt acggagaact cgcgatcccg ctcgtatcgg aagacatgca
     1921 tgtgccactg gttcggcatg cggaattgca ggtcgccggg cgatacgagc actacagcga
     1981 tgtcgggagc gtcgccaaac ccaaggtcgc gggcgcttgg gacgtatttg acggcgtgcg
     2041 cttccgtgga tcctgggcac agggcttcaa ggcgcccaat cttgaacagc tcaatgcaac
     2101 gtcggtcacg cgtaccaata cacgcacaga ctggatacgt tgcgaagccg agacccgtct
     2161 tcccgccgac aatccgcttc acatcgatag tttcacagat tgcgcagaac gcttcagtac
     2221 tccggaacaa cgggcgggca acaaagaact caaacctgaa acgagcgaaa cttggtcggt
     2281 cggcgcggtc ctgcagccca agttcattcc gcatgacttc ggtcgattca ctttcacggt
     2341 cgatctttgg catgtgaagc aaaatggcat tgtgggcgtc ttcggcggag ccaatgccct
     2401 gattgaagat tattatgatc gcctgaacgg ggccagcaat acaaacgtcg ttagggctgc
     2461 gccgacagcg gaagatgtcg ctctgttcac cgccgccggc atggacccgg tcgggcagat
     2521 actctcggtc aaggatcagt atcggaatct gttgccacaa acagcgtcgg gaatcgactt
     2581 tggatttaat tggcagctac ccaatacgtc ggttgggagt ttcagtttcg atctcaatgt
     2641 cgcttatctc cgcaaattct accgctctcc aataccagaa gtagcggcgc ttatggccgc
     2701 acgagatgcg ggggaaatca atccaggcac gacatttagc caagccgggg gagatttgat
     2761 cggccaagga agtcgtccga agtggaaggg tagcgcatca ctgacttgga gctatcagca
     2821 attccaagtg ggcgctttca ctcaatatgt cggatctgtc tatgatcccg atctgattga
     2881 cgatctcggt gaatattggt cggtcgacag ccagctcacg ggcaatctct att
//
LOCUS       DATMTI010000144         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_811947, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000144 DATMTI010000000
VERSION     DATMTI010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811947"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..2398
                     /locus_tag="VN039_02390"
     CDS             137..2398
                     /locus_tag="VN039_02390"
                     /inference="COORDINATES: protein motif:HMM:NF022063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HWV44867.1"
                     /translation="MTTSIKILKTIKPPLRWVPEQRDFTTEFLQSKSKDPNGPNYEDL
                     TKGPDSVLQEAQRILGRCLPPTAPGTPETGLVVGYVQSGKTLSFETVIALARDNGYGV
                     VIVLAGTKNNLRDQSEERLKKDFGIEDGGDHWYHWSNPTMSVKSQIENRLAAWKRKPT
                     KKALLITVLKHGGHLEKLATVLKSVDLSTVPCLIIDDESDQASLNTKAARIKAGLAAI
                     TDVSTTYKSILTLRDVLPHHSYLQYTATPQANLLLAQTDFLNPNFAELVTPGSAYTGG
                     KVFFLDNPNLIADIPPRQVPGPNNNVTSPPKTLLDALRFFLLAAAHHSLTREKGLKAK
                     DRNRSMMVHPAIATTSHKAYKAWIDKALKTLKSTVERQFASDPVAATLLFNAEYISLA
                     KTFSKIRPLPELVATMIDDVFDDLNVVEVNGTKDAEKKVNWKATKYWILVGGAKLDRG
                     YTVEGLCTTYMPRPLGTSPAADTLQQRARFFGYKAPYLGLCRVFVQATVKTAFTDYIE
                     HEEFVRNALAANRGQPLARWRRDFILTELLKPTRANVIGLGMRRIPVNGWLVPSVMQR
                     DENATAANRALLSSVVPKWEKAYGSPVNAATILQGVINSPHDVIEAVPLLAVLEDFLL
                     AVQVRDPSDAEEHSAMLIALSSMLAKDASLKVDVFLMNRLQIGYRTRVAGHGLPASHL
                     FAPINQYFSQSAGTVNDRNHCFGDRISLQLRKFSLGTHQRDPSKADIKDVTWFALNVP
                     AKLRKALLIEERR"
     gene            2400..3365
                     /locus_tag="VN039_02395"
     CDS             2400..3365
                     /locus_tag="VN039_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK motif protein"
                     /protein_id="HWV44868.1"
                     /translation="MDLFAGFTNLPLAQSTADLAAVALSAQRRDFLAKAADGSPVFLL
                     HDASPATYSPAIELKNISVQFHSTCRVTTANGTLEDQFAVIACDAAVPELHEVFIRCF
                     SATVEQLPAASSTESLQHCIQTLLDLFRVLSLPGSREVAGLWAELFVISKCNNVAQAL
                     RAWHSAPFERFDFSWPNGCLEVKATVMEQRVHEFALEQLQPPISGGGFVASVLLQSMN
                     GGVGVTDLAHTIEQYVSIEPQLRQKLWENVAVALGSDFSERLDRRFDPSFAERSLSIY
                     AMGDVPAPEQPHDSRITGMRFRVDLSSVQSSLAGSPRNLLHGLFE"
BASE COUNT          837 a         1050 c          960 g          836 t
ORIGIN      
        1 tcgccaccgc agtttgcatc gcgctggtcc tttcggagga catcacaggg cagacccccg
       61 aggccgtcct ataccacttc aactacctga tgcgtggtgt tttgagctgt gcttcgataa
      121 ccaaggatga ccaacgatga ccacttcaat caaaattctc aaaaccatca aaccgcctct
      181 ccgttgggtg cctgagcagc gcgacttcac tacggagttt ctgcagagca agtcgaaaga
      241 tccgaacggt ccgaattacg aagatctaac aaaaggcccg gattcggttc tgcaagaagc
      301 acagcgcatt ctcgggcgct gtcttccgcc caccgcgcca gggactcccg aaaccggcct
      361 cgtcgtaggg tatgtgcaaa gcggcaagac actctcattc gagacagtca ttgccttggc
      421 tcgtgacaac ggctacggcg tagtcatagt ccttgcaggt acgaagaaca atcttcgtga
      481 ccaatccgaa gaacgcctta agaaagactt cggcatagaa gatggtggtg accattggta
      541 ccactggtct aaccccacga tgtcggtcaa gagtcagatt gagaaccgat tagccgcttg
      601 gaaaaggaag ccaacgaaga aggccttact cattaccgtg ctcaagcatg gcgggcattt
      661 ggagaagttg gccaccgtcc tcaaaagtgt tgacctcagc acggtgcctt gcctcattat
      721 cgacgatgag agcgaccaag caagcctcaa cactaaggcc gcgcgcatca aggccggttt
      781 ggcagccatc actgacgtca gtacgactta taagtccatt ctgacactgc gcgatgtact
      841 gccgcaccac tcctatttgc agtacacggc cacacctcag gcgaacctgc tgctcgcgca
      901 gacagacttc ctcaacccca atttcgccga acttgtcaca ccgggtagtg cctacaccgg
      961 aggcaaggtc ttctttttgg acaatcccaa tctcattgcg gatatccctc cgcggcaagt
     1021 tcctgggcca aacaacaatg tcaccagtcc gccaaagact cttctcgatg ccttacgctt
     1081 cttcttgctc gcggcggccc atcattcgct gacgcgggaa aaggggctga aggcaaaaga
     1141 ccgtaatcga tcaatgatgg ttcacccagc catcgcaact acctcgcaca aggcctacaa
     1201 ggcgtggatt gacaaggctc taaaaacgct gaagagcacg gtcgagcgac agttcgcaag
     1261 cgacccagtc gcggcgacac tgctcttcaa tgccgagtac atatcgttag ccaagacctt
     1321 ctctaagatt cgcccgctac cagagttggt tgccacaatg atcgacgacg tctttgatga
     1381 cctaaatgtc gttgaggtga acggtacgaa ggacgctgag aagaaggtca actggaaagc
     1441 gacgaagtat tggattttgg tgggcggcgc gaaactagat cggggataca cggttgaagg
     1501 cctgtgtacc acctacatgc ccaggccgct tgggacttct ccggccgcag acacgctgca
     1561 acaacgagct cgcttctttg gctacaaagc gccatatctt ggcctctgtc gagttttcgt
     1621 ccaagccacc gtcaagacag ctttcactga ctacattgag cacgaggagt tcgtgcgcaa
     1681 cgctctggcc gccaatagag gccaaccttt ggcgcgctgg cgtcgagact tcatcttgac
     1741 cgagttgcta aagccgacgc gtgccaacgt tatcggccta ggcatgcgcc gaattccggt
     1801 caatggatgg ctcgtgccat cggttatgca gcgggatgaa aatgcaacgg ctgcgaatcg
     1861 agccctactt agttcagttg tccccaagtg ggagaaggcc tacggctcac ctgtaaacgc
     1921 ggccacgatc ctccaaggag tgattaactc tccgcacgat gtgattgaag ccgtgccgct
     1981 gttagccgtt cttgaagact tcctactggc tgtccaagta agagacccga gcgacgcaga
     2041 agagcacagt gcaatgctga ttgcgttgtc ctcgatgctc gccaaggacg catcgctcaa
     2101 ggtagatgtg ttcctgatga accggctgca aatcggttac cgtactcgcg ttgcaggtca
     2161 tggactacct gcaagccatc tttttgcgcc catcaatcag tatttcagcc aaagtgctgg
     2221 aaccgtcaac gaccgaaatc attgtttcgg cgatcgcatc agcctgcagc tgcgcaaatt
     2281 cagccttggc acgcaccaac gagacccgag caaggccgac atcaaggatg tgacttggtt
     2341 cgcgttgaat gttccagcga aattacgcaa agcgcttttg atcgaggaac gacgctgaga
     2401 tggatctgtt tgccggcttc accaaccttc ccttggcgca atccactgcg gatctcgctg
     2461 ctgttgccct gtcggcgcaa cgacgcgact ttcttgccaa agcggctgat ggcagcccgg
     2521 ttttcttgct acatgacgca agccctgcga cttactcccc cgcgattgaa ctgaagaaca
     2581 tctccgttca gttccacagt acctgccgag tgacgacggc caatggcact cttgaggatc
     2641 agtttgcagt cattgcatgt gacgcagcgg ttccggagct acacgaagtt tttatccggt
     2701 gcttttcggc gacggttgag cagctaccgg cggcatcgag caccgaaagc ctgcaacatt
     2761 gcattcagac gttactagac ctctttcgcg tgctgagcct gcctggtagt cgagaggtcg
     2821 ctggattgtg ggccgaactg tttgtgattt ccaagtgcaa taacgtggct caagcgctgc
     2881 gagcttggca ctcggccccc ttcgagcgtt tcgacttctc ttggcccaac ggttgccttg
     2941 aagtcaaggc gactgtcatg gagcagcgcg tgcatgagtt cgcgcttgag cagttgcaac
     3001 cacccatctc aggtggtgga tttgtcgctt ctgttctcct tcagtcaatg aatggagggg
     3061 tcggcgtgac cgatttggcc cacaccattg agcagtacgt ctccatcgaa ccccaactca
     3121 ggcagaagct ctgggagaac gttgcggtag cacttggaag tgatttttcg gagcgactag
     3181 accgccgttt cgacccgtca ttcgcagagc gcagtctgtc catctatgcg atgggtgatg
     3241 ttccggcacc cgaacagcct cacgattctc gaatcacagg catgcgcttc cgggtggatc
     3301 tttcatctgt gcagtcgtcg ctagctggaa gtccaagaaa tttgcttcat ggcttgttcg
     3361 agtgaatggc tcgcgcgccc taatcgtgat atcgctgacc gggttttagg aggccacgtg
     3421 catagccacg tgcgtatgtt tctatggctt caattgaatt tttgagttga gtcatacgcc
     3481 ttccccgtgt aaccgcctcc tccgccaaag caattttctc aacgctcgtc cctatgtagg
     3541 ttctggatcg ctcttgcccc ggagccctca catggaggta ggcataatga ccattgcgaa
     3601 gccatatccg tgcgtgttca cagtggggct gacctctcaa cagcaataac tctttgcaca
     3661 gctttgcatg ctgcgcaact agc
//
LOCUS       DATMTI010000145         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_303059, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000145 DATMTI010000000
VERSION     DATMTI010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303059"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VN039_02400"
     CDS             complement(<1..516)
                     /locus_tag="VN039_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HWV44869.1"
                     /translation="MMRWWLAAPLSVVVFVLPVWAADGPIDEKAWREVREVVKQLQAR
                     YEKTKDLQADFSQKTKIEGFERPVTSAGKVYIKKPGRLRWDYLDPAIEQIYVNQDDVK
                     VYVPEHKQVLVGKLTQMAASKAPLELLQGASKLDESFEIEPTTGKDRGVGGTPLITLI
                     PKAKEQESTQNL"
     gene            complement(513..>2659)
                     /locus_tag="VN039_02405"
     CDS             complement(513..>2659)
                     /locus_tag="VN039_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWV44870.1"
                     /translation="LGFRCFSQAPVSLRLRTAASSLAAVFFLSAFLHLETTGVPTLTS
                     GMISRGMAGGVVGQIIADGLRAIFAGTGAHILIIAGFLVSLLLTTPLSLAEVVRRMPE
                     RWATLREGVSAVMPERSTAVQKEAGNRRSRVKPSRSSTVPLEEESDAERIEDAEPIPA
                     STPLTPIIQPFPVTTPTPQGQTTEPEVVVPPSEPADYQLPDPEILLSDPSGAMDRMSD
                     EELRAQSEVLSRALMSFGIEGIVTEVRPGPVVTMYEFEPAPGTKVARIVNLADDLALA
                     LKATSLRIVAPLPGKSVVGIEVPNRSRETVSLKEVVMSEAFRRARSRLTLALGKDIFG
                     APMTADLKTMPHLLVAGATGAGKSVSLNTMLLSILFSAKPSEVKLLLIDPKMLEFQSY
                     EGIPHLLRPVITEPKSAARGLGWVVAEMERRYKLLAEAGVRNIDAYNRKVAGLHEVFA
                     ENNPAGIEQTELPMQFLSEEERLSAGETSIAEGERGCMQPKPTPPEPLPFIVVMIDEL
                     ADLMMVAPKDVEDKIARLAQMARASGIHLVLATQRPSVDVLTGLIKANFPARIAFQVS
                     SKTDSRTILDANGAEALLGRGDMLYLASGTGRLARLHGSFVSDDDVRSVVEFVKKQAL
                     PIYNQELQSLKLEEAAEEEAKDEVYEQAKELVLSTGQASASLIQRRLRVGYPRAARMI
                     EQMESEGIVGAAGRDGRREVLGRRGPVGAAEA"
BASE COUNT          551 a          841 c          697 g          570 t
ORIGIN      
        1 aagattctgg gtggattcct gctccttggc tttgggaatc agtgtgataa gcggcgtacc
       61 accgactccc cgatcttttc ccgtggtggg ctcaatctca aacgactcgt ccaatttgga
      121 cgctccctgc aataattcca acggcgcttt ggaggcagcc atctgcgtca gtttcccgac
      181 taggacctgt ttatgttcgg gaacgtacac cttcacatcg tcctggttca catagatttg
      241 ctcgatcgcc ggatccaaat agtcccaccg taatcggccg ggctttttga tatacacctt
      301 gccggccgac gtcaccggcc gctcaaaccc ctcaatcttg gttttctgag aaaaatcggc
      361 ctgcagatcc ttcgtttttt cgtatcgtgc ttgcagctgt ttgacgactt cgcgaacttc
      421 ccgccatgcc ttttcgtcaa tcggcccgtc tgccgcccaa accggcaaga cgaacacaac
      481 aacactcaat ggagcggcaa gccaccaacg catcatgctt ccgccgcgcc gaccggaccg
      541 cgccggccaa gcacctcccg acgcccatca cggcccgccg cccccacaat gccctccgat
      601 tccatctgtt cgatcatgcg cgccgcccga ggatagccga ctcgcagtcg gcgctgaatc
      661 aacgaggctg aagcctgccc ggtcgacagg accaattctt tagcctgttc gtacacttca
      721 tcttttgcct cttcttccgc cgcctcctcc aacttgagcg actgcagttc ctgattgtaa
      781 atcgggaggg cttgtttctt cacgaattcc acgactgagc gaacatcgtc gtccgataca
      841 aaggagccgt gcagccgtgc gaggcggccg gtgcctgagg ccagatacag catatcgcct
      901 cggccgagta gagcctccgc tccgttcgca tcgagaatgg ttcgtgagtc ggtcttcgaa
      961 gatacttgaa acgcgatccg tgccggaaaa ttcgctttga tcaagccggt cagcacatcg
     1021 acggacgggc gctgagtggc caacactaga tgaatgccgg aagcccgcgc catctgtgca
     1081 agtcgggcga tcttgtcttc cacatctttg ggagccacca tcatcagatc cgcgagctcg
     1141 tcgatcatga cgacgataaa cggcagcggt tccggcggcg tcggtttcgg ctgcatgcaa
     1201 ccgcgctcac cctcggcgat cgatgtttca ccggcagaga ggcgctcttc ttcagagaga
     1261 aactgcattg gaagctcggt ctgctcgatg cccgccgggt tgttttcggc aaacacctca
     1321 tgcaaaccgg caacctttcg attgtacgca tcgatgttcc gtacaccggc ctcggccagc
     1381 agcttgtacc gccgttccat ttcagcgacg acccacccca gccctctcgc ggctgatttg
     1441 ggctccgtaa tcaccggtcg caacagatgg ggaatgccct cgtacgattg aaattcgagc
     1501 atcttcgggt caatgagcag gagcttcact tcactgggtt tggcggaaaa gaggatactc
     1561 agcaacatcg tattcaaact gacactcttc ccggcaccgg tcgcaccggc taccaagaga
     1621 tgcggcatcg ttttcaaatc ggctgtcatc ggtgccccga agatatcctt gcccaacgcc
     1681 agagtcagtc tcgatcttgc ccgacgaaag gcctcgctca tgacgacttc cttcagcgac
     1741 accgtctctc gagatcggtt cggcacctcg atgccgacca ctgatttccc aggcaacggc
     1801 gccacgattc gtagactcgt cgccttgaga gccagcgcaa ggtcatcagc cagattcaca
     1861 atgcgggcca ctttcgtgcc gggtgccggt tcaaactcgt acatcgtaac gactggaccg
     1921 ggccttactt ccgtcacgat gccctcgatg ccgaaactca tcaatgctcg tgacaaaacc
     1981 tcggactgcg ctcgtaattc ttcatccgac atccgatcca tcgctccgga aggatcgctt
     2041 aacaaaatct ccgggtcagg caactgataa tccgcaggct ccgagggtgg gacaaccact
     2101 tccggctcgg ttgtttgccc ttgcggtgtt ggggttgtga cagggaacgg ctgaatgatc
     2161 ggagtcagcg gagtcgaggc tggaattggt tccgcgtcct cgattctttc ggcatccgac
     2221 tcttcttcca gggggacggt gcttgaccgg gaaggcttaa cccgcgaccg tcgattgccg
     2281 gcctccttct ggaccgccgt cgatcgttca ggcatcaccg cggacactcc ttcgcggagt
     2341 gtggcccaac gttccggcat ccggcgcacg acttctgcca gtgacagagg cgttgtcagc
     2401 agaagagaca caagaaatcc cgcaatgatc agaatatggg ctccggttcc ggcaaagatg
     2461 gcacggagtc catcggcgat tatctggccg acgacaccac cggccatccc tcgggaaatc
     2521 atcccgctgg tcagcgtcgg aacgcccgtc gtttcaagat gcagaaatgc actcaagaag
     2581 aagacggcgg ctagggaact ggcggccgtc cgcagcctga gactcaccgg cgcctgggaa
     2641 aaacagcgaa atcccaacc
//
LOCUS       DATMTI010000146         4518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_259957, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000146 DATMTI010000000
VERSION     DATMTI010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259957"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VN039_02410"
     CDS             <1..890
                     /locus_tag="VN039_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44871.1"
                     /translation="IKSLKNVSSQMNLIAGSHVDGYEATIAALKKEIKFLKGAVGENP
                     TVQLYLYDPDHSRIIQILGTPSADTSHVITYVPGTFTNIDSFYDKSSQQFAAQLLTGY
                     PDAVAFVYKDGVFPGGDDGTDAINYARIGEANDPARALAAGRQLAQFEQGLSTDPTLA
                     AAQSTAIGHSWGLTNITSSEISGAHYNQVISLSGAGMPAGWEASPSTSYTDYSYYDIL
                     QEAQGLGLVWGGDVPRADPAFTHGPYYQGPVDPIEYGPFGAPDGVDQGVLLNNHNLIA
                     TNTPANAKVLKDVIREVYQ"
     gene            962..1405
                     /locus_tag="VN039_02415"
     CDS             962..1405
                     /locus_tag="VN039_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44872.1"
                     /translation="MSSSGPADRTISSLSLEQAKHVAQRMEDTIAGLIPNEYVAKRDQ
                     VSKGSLLSCSKDSYQWSGRTFVHLQGSPNVDQILDRIATHWKGDPDYTIDRKPAADGT
                     PSIAFSGAHGSVYLVGPTLNQEQVQIMSFSPCFRLAEDADPGGTF"
     gene            1534..1977
                     /locus_tag="VN039_02420"
     CDS             1534..1977
                     /locus_tag="VN039_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44873.1"
                     /translation="MSGSGPSDRTISSLNVEQAKHAAQKMEDTIAGLVPDEYVAKRDQ
                     VPKGSLLSCSKDSYQWSGQTDVYLQGSPDVDGILDRIAQHWKGSENYTVIRGKAGDGT
                     PQVRIDGAHNAGYIVGPWKSNSQLLILSFSPCFRLPEDADPGGTF"
     gene            2097..2540
                     /locus_tag="VN039_02425"
     CDS             2097..2540
                     /locus_tag="VN039_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44874.1"
                     /translation="MSASGPADRTISSLSLEQAKHAAQKMEDTIAGLVPDEYVAKSEQ
                     APKGTLLSCSKDSYQWSGQTDVYLEGSPDVDRILDRIADHWKGDDNYTIKRDTAANNT
                     PRLTLSGAHGAGYIVNPTRDNSHVQILSFSPCFRLPDDVYPGGTF"
     gene            2654..2953
                     /locus_tag="VN039_02430"
     CDS             2654..2953
                     /locus_tag="VN039_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44875.1"
                     /translation="MPDSWSPDTGTTHTDYSYNDILQAAQRTGIVGDGNDPRLNPAFN
                     HGPYYHAPGDISVYNTDGQPEQANPLNWVADHNLVGTNNPDNRQVVVDVAREIYR"
     gene            3034..3456
                     /locus_tag="VN039_02435"
     CDS             3034..3456
                     /locus_tag="VN039_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44876.1"
                     /translation="MHDGSLTVERAKASTLAAEDEIVSLVPTKYVADREQKSEAHLFR
                     CSGTGYSWPGHTTVKLRSGADREAIIDAIVKHWAAKPGYSVSRSTTKPDNEPRITIQG
                     HNGATYYVEFWYKDTQLQISSFSPCFNHEPGQSLNDTY"
     gene            3614..4144
                     /locus_tag="VN039_02440"
     CDS             3614..4144
                     /locus_tag="VN039_02440"
                     /inference="COORDINATES: protein motif:HMM:NF018018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV44877.1"
                     /translation="MSEGSGMSLNMVAHSYGTTTASLGLAGEDLHVDSFVSLGSAGIP
                     QSVPTADAIHADHVYAGQAQNAIPGLEKGDEWAWRGRDGSGRLNPMDPSFGATTFNTD
                     GVPGDDSLHGITEHATGKKPTGYGYLDLKTESLNNTALATTGHGDNVSPYQNPGYTDL
                     QKTLIRSMEQERSYGY"
     gene            4134..>4518
                     /locus_tag="VN039_02445"
     CDS             4134..>4518
                     /locus_tag="VN039_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44878.1"
                     /translation="MGIERSRKPLLKSALLAGTVLVLLAGCVQGGKVPTMTPDESKKQ
                     MLDLIDDTISVLNSTGWSDPGDIAPIDCVLPSGATGVNYAATTMGPLSSDPEADIKIV
                     EAFWKKKGFTTKVVHSTNPKDTQIWL"
BASE COUNT         1034 a         1321 c         1350 g          813 t
ORIGIN      
        1 tgatcaagag tctcaagaat gtgtcgtcgc agatgaacct cattgccgga agccacgtgg
       61 acggttacga agccacaatc gcggccctga agaaagagat caaattcctg aagggcgcag
      121 tcggcgaaaa cccgactgtg cagctttacc tctacgaccc agatcactcg cgcatcatcc
      181 aaatactcgg gaccccctcc gcggacacca gccacgtcat cacctacgtg cccggcacat
      241 tcacgaacat tgactccttc tacgacaaga gctcccagca attcgccgcg caattactga
      301 ccgggtatcc ggatgcggtg gcgttcgtgt acaaggacgg cgtgtttccg ggcggcgacg
      361 acggcaccga cgcgatcaac tacgcgcgga tcggcgaggc aaacgatccg gcccgtgccc
      421 tcgcggcagg acgccagctc gcacaattcg aacaaggact aagcaccgac ccgacactag
      481 cggccgctca atcgaccgca atcggtcaca gttgggggct gacgaacatc acctcctccg
      541 aaatctccgg tgcccattac aaccaagtca tctcgctgtc gggggcaggc atgccggccg
      601 gctgggaggc aagcccgtcg acctcataca ccgactactc ttactacgac atcttgcaag
      661 aagcgcaagg actaggcctc gtgtggggtg gcgacgttcc ccgagcagac ccggctttca
      721 cccacgggcc ctactatcaa ggaccggtag atcccatcga atacggaccg ttcggagctc
      781 ccgacggagt cgaccaagga gtcctgctga acaaccacaa cctcatcgcc accaacacac
      841 cggcaaacgc gaaagttctc aaggacgtga ttcgcgaggt gtatcaatga taaacgtgcg
      901 ccaattcggc ggaattgtgg caacggtgct ggtcgtggtc gcatgtttgg gagggtgctc
      961 aatgtcaagt tctggaccgg cagacaggac gatcagctcg ctaagcctgg aacaggccaa
     1021 gcacgtcgct cagaggatgg aggacacgat cgccggactg atcccgaacg aatacgtcgc
     1081 gaaacgggac caggtttcga aaggctcgct tctgtcctgc tctaaagact catatcaatg
     1141 gagcgggcga acgtttgttc acctgcaagg atcgcccaac gttgaccaga tccttgaccg
     1201 gatcgccacg cattggaaag gggaccccga ctacacgatc gaccggaaac cggccgccga
     1261 tgggacgccg agcatcgcat tttcgggagc tcacggctcc gtctatctcg tcggaccgac
     1321 ccttaatcaa gaacaggtac agataatgtc gttctccccc tgcttcaggc tcgcggagga
     1381 cgccgacccg ggtgggacgt tctgactgcc gtgcggcatg acgcaacgtc gagatacaag
     1441 gtccgacagc ccggcgcatc agggagccac aagccgctcg cccttagtgt gacggcagcc
     1501 gcggtcgtgg ccgtgtgttt gggagggtgc tcaatgtcag gttcgggccc gtcggatagg
     1561 acgatcagtt cgttgaacgt ggaacaggcc aaacacgccg cacagaaaat ggaagacacg
     1621 atcgccggtt tggtccctga cgagtacgtt gcgaaacgcg atcaggttcc gaaaggctcg
     1681 cttctgtcgt gctcgaaaga ctcgtaccag tggagcgggc agaccgatgt ttacctgcaa
     1741 gggtcgcccg acgttgacgg gatccttgat cggatcgctc agcattggaa agggagcgaa
     1801 aactacacgg tgatccgtgg aaaagctggt gacggcaccc cgcaagtgag aatcgacggt
     1861 gcccacaatg ctggctatat cgtcgggccg tggaaatcca atagccagct actgattctg
     1921 tcgttctcgc cgtgcttcag gttgccggag gacgccgacc cgggtgggac gttctgacta
     1981 tggcgcggca agaggcaacc tcgagaccgc tctgcgacgt atcggccggt tggaatcgac
     2041 gcgccctcac ggtgtcgata gcagcgctcg tggcggtgtg tctaggagga tgctcaatgt
     2101 cagcttctgg gccggcagac aggacgatca gctcgctaag cctggaacag gccaagcatg
     2161 ccgcgcagaa aatggaagac acgatcgctg ggctggtccc ggacgaatac gtcgcgaaaa
     2221 gcgagcaagc gcccaagggt accctgctgt cgtgctccaa ggactcgtat cagtggagcg
     2281 gacaaacgga tgtttacctg gaagggtcgc ccgatgtgga ccggatactt gatcgaatcg
     2341 ccgaccattg gaaaggggac gacaattaca cgatcaagcg cgatacggcc gccaataaca
     2401 ctccccgtct caccctctcg ggcgctcacg gggcaggtta tatcgtcaac ccaacccgtg
     2461 acaattcaca cgtgcagatc ttgtcattct ccccctgctt caggctgccg gacgacgtgt
     2521 acccgggcgg gacgttctga ctgccgcgcg ggataatcca acctcgaagt acgaggtccg
     2581 gcagcccggc gcatcacgga gccacaagcc acgcgccctc acgatgcaag tcatctcgtt
     2641 ctccggagca tggatgccag actcgtggtc accagatacc gggaccaccc acaccgatta
     2701 ttcgtataac gacatactgc aagccgcaca gaggacgggg atcgtcggcg acggcaacga
     2761 cccacgcctg aacccggcgt tcaatcacgg cccgtactat catgcaccag gggacatttc
     2821 ggtctataac accgatggtc aacctgagca ggctaacccg ctgaactggg ttgcggacca
     2881 caaccttgtc ggaacgaaca accccgacaa ccgccaggtg gtcgtcgatg tcgccaggga
     2941 gatctaccga tgaggcgcag gcgttggcca ttaattctga tcgtcgcgct caccgcaggg
     3001 ttaagcctgg cgggatgttc aaatggaggc cgcgtgcacg atggttcgtt gacagttgag
     3061 cgcgcgaagg cgagcaccct ggccgctgaa gatgagatcg tatctttggt gcccacaaaa
     3121 tatgtggctg atcgcgagca gaagtctgaa gcccacctgt ttcgatgttc cgggactggt
     3181 tacagctggc ccgggcatac gaccgtgaaa ttgcggtcgg gcgccgatcg tgaggcgatc
     3241 atcgacgcga ttgtgaagca ttgggccgcg aagcccggct actccgtatc gcggtcgaca
     3301 accaagccgg acaacgagcc gcgcatcacg atccaaggtc acaacggtgc gacgtactat
     3361 gtcgagtttt ggtacaaaga cactcaactg cagatttcgt cattctcgcc gtgcttcaac
     3421 cacgaaccgg gccaaagcct caacgacacg tattgagcat cgcgaattcc gacgagtgtc
     3481 gcattgtagg aggcgtgtat gggccatgcc ctgcccgtcc ccctgggctg aaatctggac
     3541 gttgtgtcgt gcgaaatgcg gatgcgcaag ccggtagggt gcacctggat catgatctcg
     3601 agggtttgaa tgtatttcgg aaggatcagg gatgtcgctg aacatggtgg cacactcgta
     3661 cgggacgacc accgcatcgc tgggcctggc tggggaggac ctgcacgtgg actcgttcgt
     3721 cagtctgggc tcagccggta ttccgcagtc agtgcccacc gcggatgcga ttcacgcgga
     3781 tcacgtctat gccgggcaag cgcagaacgc tattcctggt ttggagaagg gtgacgagtg
     3841 ggcgtggagg ggccgcgacg gtagcggacg actgaacccg atggacccaa gcttcggtgc
     3901 caccacgttc aacacggacg gtgtgcctgg cgacgactca ctgcatggaa taaccgaaca
     3961 cgccaccggt aagaaaccaa ccgggtacgg atacttggac ctgaaaacag agtcgctcaa
     4021 caacacggcg ctggcgacaa ccgggcacgg tgacaacgtg agcccctacc agaacccggg
     4081 atacacagat ttgcagaaga cgctgatccg ctcgatggag caggagcgca gctatgggta
     4141 ttgagcgatc gagaaaaccg ctcttgaaga gtgctctcct ggccgggacc gtgctcgtgt
     4201 tacttgccgg atgcgtacag ggtgggaagg ttccgacgat gacgcctgat gaatcgaaga
     4261 agcagatgct cgacctgatc gatgacacga tcagcgtgct caactcaaca ggatggtccg
     4321 atcccggtga catcgccccg atcgattgcg tgttgcccag cggtgcgacg ggggtgaatt
     4381 acgcggccac cacgatgggt ccgctgagta gtgacccgga ggctgacatc aagattgtgg
     4441 aagcgttttg gaagaagaag ggcttcacca ccaaggtggt gcactctacg aacccgaaag
     4501 atacgcaaat ctggctct
//
LOCUS       DATMTI010000147         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_812034, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000147 DATMTI010000000
VERSION     DATMTI010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812034"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..497)
                     /gene="rpsI"
                     /locus_tag="VN039_02450"
     CDS             complement(105..497)
                     /gene="rpsI"
                     /locus_tag="VN039_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWV44879.1"
                     /translation="MAVVTQYATGRRKCAVARAWVTGNTGDIVVNDKPLEKAFPRLTL
                     RQIIQLPLEMAGLMGKYSISATVYGGGPTGQAGALRHAIARALVAMTPATRSSLKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            complement(531..959)
                     /gene="rplM"
                     /locus_tag="VN039_02455"
     CDS             complement(531..959)
                     /gene="rplM"
                     /locus_tag="VN039_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWV44880.1"
                     /translation="MMTYFENPIHVKETWHLVNAEGKTLGRLAARVASVLRGKHRPTF
                     TPNVDMGDHVVIVNAEKIQLTGDKMETKLYRRHTGYPGGLKTASAAHVFRKDPTQLLT
                     KAIKGMLPKTPLGNGMARKLRVYVGPNHPHHAQRPQPISL"
     gene            complement(1004..1492)
                     /locus_tag="VN039_02460"
     CDS             complement(1004..1492)
                     /locus_tag="VN039_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWV44881.1"
                     /translation="MEEHATQTAEPLIQLTVNRVVEDSTTDTRIVVLTRADGTSDHFM
                     VWVGASEGEAIRRALDTSTTPRPMSHDLIKSFGEHLGIKMERAVLTDVKSSTYYASVF
                     LENKGVARTIDSRPSDAIALALRCQAPIYVTQDVWKRRSGQNLDAWLSKLGTKNIGAQ
                     EV"
     gene            complement(1517..1972)
                     /locus_tag="VN039_02465"
     CDS             complement(1517..1972)
                     /locus_tag="VN039_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWV44882.1"
                     /translation="MITQMQVKGLMFDPYNNAYIVILRDDDQSEMLPIWVGKSEASSI
                     SLAIENVAPPRPMTHDFMKSYLDAFNAKVISVVITDLNEHTYFAKVHLTYADSEYTVD
                     SRPSDAIALALRTQAPIFASESVIRKQSSEELDQWLENLKPEDFGKLDS"
BASE COUNT          438 a          592 c          569 g          536 t
ORIGIN      
        1 ctcatgcgtc accatctcct ggtcgacggt cgattcacat caagcagaca caaaaaaggg
       61 gaaggcatgg agccttcccc tttttggatc gtcggcccct gcgcctagcg cttggagtat
      121 tggaaccgct tgcgcgcgcc cttctgtccg tacttctttc gttccttcac gcgtgagtca
      181 cgcgtgagca acccctcctt cttcagggag ctgcgggttg caggggtcat cgcaacgagc
      241 gcccgtgcga tggcatggcg gagcgcaccg gcttgtccgg tcgggccgcc cccatagacg
      301 gttgcgctga tcgagtattt tcccatcagg ccggccatct caaggggtag ctggatgatt
      361 tgccggagag tgagacgagg aaacgccttc tccaacggct tatcgttcac gacaatgtcg
      421 cccgtattgc ctgtaaccca agcccgggcg actgcgcact ttctccgccc cgttgcgtat
      481 tgtgtcacca ctgccatgca attcgtctcc ttctgtttga ggacatgtcc ctagagagaa
      541 ataggttgag gacgttgagc gtgatgcgga tgattgggtc cgacatagac gcggagtttt
      601 cgtgccatgc cattgccgag cggggttttg ggcagcatgc ccttaatcgc ttttgtcagg
      661 agctgcgtag ggtctttccg aaacacatgc gcggcggagg ccgttttcaa ccctccagga
      721 tagccagtat ggcgccggta cagtttggtt tccatcttgt cgccggtcaa ctgaatcttc
      781 tccgcattca cgatgaccac atggtctccc atatcaacat tcggcgtgaa tgtcgggcga
      841 tgtttccctc gcaaaacgct cgccactcgt gccgccaatc gacccagggt tttcccctcg
      901 gcgttcacca gatgccaggt ttctttcaca tgaatcggat tttcgaaata cgtcatcatc
      961 tttttctcca ccgctgcaac tctgtcagca tatgataagg atgttagact tcttgggcgc
     1021 cgatattctt cgttcccagc ttggacaacc aggcgtccag attttgaccg ctgcgtcgtt
     1081 tccacacatc ctgcgtgaca taaataggcg cctgacagcg gagggccaag gcaatggcat
     1141 cgctcggtct ggaatcgatg gtacgtgcga ctcctttgtt ttcgaggaag acgctcgcat
     1201 agtatgtgct gctcttgacg tcggtgagaa ccgcccgttc catcttaatg ccgaggtgct
     1261 cgccgaaact cttgatcaga tcgtgactca tcggtcgtgg cgtggtcgat gtgtccaggg
     1321 cgcgtctgat ggcttcacct tccgatgcgc ccacccatac cataaaatgg tccgacgtgc
     1381 cgtcggctcg ggtcaacacg acaatcctgg tatccgtcgt cgagtcttct acaactcgat
     1441 tgaccgtaag ctgtatcaat ggctcggccg tttgcgtcgc gtgctcttcc atacatgcaa
     1501 caataaagac agttcgtcag gagtccaatt ttccgaagtc ctccggcttc aggttttcca
     1561 gccactgatc gagttcctcc gagctctgtt ttcgaatcac agactcactc gcaaaaattg
     1621 gagcctgggt ccggagagcg agggcgatgg cgtcgctggg acgggaatcg acggtatact
     1681 ctgagtctgc ataggtcaaa tgcactttgg caaaataggt atgttcattc aggtccgtaa
     1741 tcaccacact gatgaccttg gcgttgaatg catccaagta ggatttcatg aaatcgtggg
     1801 tcatgggacg tgggggggcg acattctcga tcgccaggct gatcgagctt gcttccgatt
     1861 ttccgaccca gatcggcagc atttccgact gatcatcgtc tcggagtatg acgatatacg
     1921 cattgttata ggggtcaaac atgagcccct tgacctgcat ctgtgtgatc atcttctgag
     1981 ccggaatcct ctgtccatca tttagttgac gatggaacgg cagagggtgt caccctagca
     2041 ctttcggata agcaatgtca acgaaccaag cgagaatcca agggaaaatt ggaatctcac
     2101 ttgctaaagc tcttccggaa gaaatgcgca gtcag
//
LOCUS       DATMTI010000148         1904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_812066, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000148 DATMTI010000000
VERSION     DATMTI010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812066"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..535
                     /locus_tag="VN039_02470"
     CDS             146..535
                     /locus_tag="VN039_02470"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HWV44883.1"
                     /translation="MRHVGRFLVIALLLGSTGCTIKATLNQTTDTTSNITGTTSGAAW
                     WSEDGQIKPDFKATAFVTFNQANLSQDLAAGRGEYLGSVSRLLGVPSDRQPAFFSAAQ
                     ANYAQMVDLNSEALFTLLRETSKPFVY"
     gene            complement(546..>1904)
                     /locus_tag="VN039_02475"
     CDS             complement(546..>1904)
                     /locus_tag="VN039_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44884.1"
                     /translation="LSPQPASSAQRGESSTLSGADAPEWCHRRLLARIHRLTIGILRK
                     EVEPVTATEFMRFLLQWQHVVPGSRQHGEGGLLQVIGQLAGFEAAAAAWEPQLLRLRM
                     AKYESELLDRLCLSGAIGWGRLSPHPKFSQAGETERRRIVPTSLAPVSLFLREDREWL
                     MDAARRESTPSLADLHSQLSAIAQDLYRVLQQQGASFFADLVRMTEHLPAEVENGLWE
                     LVAAGLVTADGFDNLRALIDPHRRRAEGRERTRRPRHSSGRWSLLRPVNQSLVNGHWS
                     SALDQTISYQPSAIGSSASTRHSTLNAQHCERTARLLLRRYGVVFLDMLARESLVQSW
                     RDLLVQYRRMEMAGKVRGGRFVNGFTGEQFALPEAVEALRTIRKKSPSAGAEQEIKLS
                     ATDPLNLTGVILPGPRVPAVPTNFLVFKGGTLSRTVIGRQGDAAHFVAEQTGPATVRQ
                     PA"
BASE COUNT          418 a          549 c          541 g          396 t
ORIGIN      
        1 ggaaatctcc tgatcgagac tactaggtac ttacccgtcg cttccctatc ggtataggat
       61 cggcttaact taatggactg tatgttatat tgtgcgcact cggtcatcgt catccgtttc
      121 tgccatgaag aaggaggagg actttatgcg gcacgtgggt cgattcctag tgattgctct
      181 gttgctcgga agcactggtt gcacaattaa ggccaccttg aatcaaacga ccgacacgac
      241 gtcgaatatc accggcacaa cgtccggagc ggcctggtgg agcgaagacg gccagatcaa
      301 gccggacttc aaagccacgg cgtttgtgac ctttaatcag gcgaatctga gccaggacct
      361 ggcagccgga agaggagagt atctgggatc cgtgagtcgt ctgttgggtg ttccaagcga
      421 ccgacaaccg gcattttttt ctgccgccca ggcgaattat gcccaaatgg tggacctaaa
      481 ttccgaagcc ttgttcactc ttttgcgcga gacgtccaaa ccattcgtgt actgacctgc
      541 taaattcatg caggctgtcg aaccgttgcc gggccggtct gctcggcaac aaaatgcgcc
      601 gcgtcacctt gccgtccgat cacggtgcgg gaaagtgtgc cgcccttgaa taccagaaaa
      661 ttggtcggca ccgctgggac acgagggcca gggagaatca ccccggtgag gttcaatggg
      721 tctgtagctg agagcttgat ctcctgctcc gccccagcgg agggactctt ttttcgtatc
      781 gttcgcagag cttccaccgc ttccggcagc gcgaattgtt ccccggtgaa tccgttcaca
      841 aatcgaccgc cgcggacctt ccctgccatc tccatgcgcc gatactgcac aagcagatca
      901 cgccacgact gaaccaatga ttcccgcgcc agcatatcga gaaacacaac cccatagcga
      961 cgtagtaaca ggcgagccgt gcgctcgcag tgctgagcgt tgagtgttga gtgccgagtt
     1021 gaagcagaag agccgatggc tgatggctga tagctgatgg tttgatccag agccgatgac
     1081 cagtgaccat tgactagtga ctgattcact ggtctcaaca gcgaccaccg tccggatgag
     1141 tgtcggggtc gacgagttcg ttcacgtcct tcggcccgtc gccgatgggg gtctatgagg
     1201 gcacggaggt tatcgaaccc atcggctgtt acgagaccgg ccgccaccaa ttcccacagt
     1261 ccgttttcga cctccgccgg aagatgctcg gtcatccgca ccagatcggc gaagaagctg
     1321 gcaccctgtt gctgtaagac acgatacaga tcctgtgcga tcgcactcag ctgagaatgg
     1381 agatcggcga gagacggggt cgactcgcgg cgagcagcat ccatcaacca ctctcggtct
     1441 tcacggagaa aaagactgac gggagcgagg ctggtcggaa caatacgccg ccgttccgtc
     1501 tcccctgctt gtgagaattt cggatgcgga gagagacggc cccagccgat cgcgccactc
     1561 agacagaggc gatccaagag ttccgattcg tacttcgcca tgcgaagacg caacagctgt
     1621 ggctcccatg cagccgcagc cgcctcgaat cctgcaagtt gtccgattac ttgcagcagg
     1681 cctccttctc catgctgacg tgatcccggt acgacatgct gccattgcag caagaatcgc
     1741 atgaactctg tcgccgtgac cggctcaacc tctttgcgca aaattccgat cgttagacga
     1801 tggatacggg ccagcaaacg gcggtgacac cattcgggcg catcggcgcc cgatagtgtt
     1861 gaggactcgc ctcgctgagc ggacgaagcg ggctgaggac tgag
//
LOCUS       DATMTI010000149         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_599671, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000149 DATMTI010000000
VERSION     DATMTI010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599671"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VN039_02480"
     CDS             complement(<1..530)
                     /locus_tag="VN039_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44885.1"
                     /translation="MQDDITPTQPNTTPPEDLTPQANAPSTPDAFATPVAGSSVEPNT
                     ASIAGDTSPTPFGPVGSGEALNTTPPSQPGSGSKKKVVIGGILAAVLVLLIGGGAAAY
                     KMWYQNPNKVVGDALLSVLDAKTGTATGTATVDSKDVSVKVTTDGKSSKDAAGSDTTI
                     TFTMKSGELAKKDFTV"
     gene            complement(599..>1502)
                     /locus_tag="VN039_02485"
     CDS             complement(599..>1502)
                     /locus_tag="VN039_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HWV44886.1"
                     /translation="VITAVSPEYMASFTNVDVLAQEELTVANFSKIALINREDIDGRF
                     ANYLTNSAIDTYGTTAAAEYRFEIADFSLTNGYSGSVMAPEFPEPQPARINVAGEHSL
                     RPAMAATAVAAKLGLSPVDIVSGLARLQSSPGRMNVLRGLQQSTIIDDTYDSNPASAA
                     SALQTLYAVEAPQRIAILGSMTGLGQFSPDEHRKIGELCDPNLLAWVVTIGDEAEAYL
                     APAARSRGCQVRSFKSPLEAGGFVHGILDNEGACILAKGSRDGVFVEEAVKVLVHSNE
                     EDQQLVRQTPEWLEMKSNLFSKFS"
BASE COUNT          331 a          420 c          399 g          352 t
ORIGIN      
        1 ttaaccgtaa agtctttctt ggcaagctca ccagatttca tcgtaaaggt gatagtcgta
       61 tccgaaccgg cggcgtcctt tgatgattta ccgtcggtcg tgaccttaac gctgacatct
      121 ttggagtcaa cggtagccgt accagtcgca gtaccggtct tggcgtcaag cacactcagg
      181 agcgcatcgc cgactacctt gttcgggttc tgataccaca ttttatacgc tgcggcacca
      241 ccgccaatca gcaatacgag tacggcggcc aagataccac cgatgaccac tttcttttta
      301 ctgccggatc ctggttgtga tggaggtgtc gtattcagtg cttctccact gcctaccggt
      361 ccgaacgggg tcggtgatgt gtccccggca attgatgcgg tgttcggctc aacgcttgaa
      421 cccgctacgg gagtcgcgaa cgcgtccggt gtcgacggcg catttgcctg gggcgtcagg
      481 tcttccggtg gcgttgtgtt tggttgtgtc ggtgtaatgt cgtcttgcat agttcctacc
      541 taaaaggcta ttacatattg tttatggtta ctgtcagtgt atcataccgg tccaggtatc
      601 aggaaaattt tgagaagaga ttactcttca tttcaagcca ttcaggagtt tgacgcacaa
      661 gctgctggtc ttcttcgttg ctgtggacca ggaccttgac cgcttcttcg acgaaaaccc
      721 cgtccctgga acctttcgca aggatacagg caccttcgtt atccaaaatg ccatgaacga
      781 atccgccagc ttcaagcgga gatttgaagc tgcggacctg acaaccacgc gagcgcgcag
      841 caggcgccag gtaagcttcc gcctcatcgc caatcgtcac aacccatgcg agaaggttcg
      901 gatcgcacag ttcgcctatc ttccgatgct cgtcgggcga aaactgaccg agtcccgtca
      961 tactgccgag tatcgcgatc cgttgaggcg cctctacggc atagagcgtt tgcagagcag
     1021 acgcggccga agcagggttg gaatcatacg tatcatcaat aatcgtcgac tgttggagac
     1081 cacggagcac gttcatgcgc cccggtgatg attggagccg cgccaagcca ctgacgatgt
     1141 ccactggtga cagaccgagc ttggcagcaa ccgccgtcgc agccatggcc ggacgcaagc
     1201 tgtgctcacc ggctacattg atacgcgcag gctgtggctc gggaaactcc ggcgccatca
     1261 ctgagccgga gtagccattt gttaaagaaa aatcagcgat ctcgaaccgg tactcggcgg
     1321 cggccgtcgt cccgtatgta tcaatcgcgc tgttggtcag ataattcgcg aaccggccgt
     1381 caatatcctc gcgattgata agcgcgatct tcgagaaatt agcaacagtc agctcctcct
     1441 gggcaagcac atccacattg gtaaacgatg ccatatattc cggagaaacc gccgtaatca
     1501 cc
//
LOCUS       DATMTI010000150         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_90534, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000150 DATMTI010000000
VERSION     DATMTI010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90534"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..751)
                     /gene="rnhC"
                     /locus_tag="VN039_02490"
     CDS             complement(89..751)
                     /gene="rnhC"
                     /locus_tag="VN039_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HWV44887.1"
                     /translation="MTASHPRSRIERIGIDESGKGDYFGPLVIAAVFVDATTQGELKL
                     MEVRDSKKLSDSRILELAPDIKTICPHSVIAIGPQKYNELYTKIKNLNRLLAWGHAKA
                     LETLLERVTCDRAISDQFGDERLILNVLQEKGRKIVLEQRTKAESDLAVAAASVLARA
                     EFLIRLKRLSGEVGTTLPKGASPAVELAAKMIIKKHGRERLDSVAKLHFKTTRSILAG
                     LI"
     gene            869..1735
                     /locus_tag="VN039_02495"
     CDS             869..1735
                     /locus_tag="VN039_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWV44888.1"
                     /translation="MQTPRVMRATTMCCLLAGGIGFVGCETNPYTGRSQLLMTSVSQE
                     MQMGAQAYNQVKSDPKMKPSQDPREIEPVKRVAARIVEAAKRSKYAEMAQQFQWEVTV
                     IKDDKTANAFALPGGKMAVYTGIFPMAKTEAGLAAVMGHEVVHALARHGAERMSQGQV
                     VNIGLQVVGAAVGISSKNPALGQATMAALGAGAQVGVLLPFSRKHESEADHIGILLAA
                     DAGYDPRESVALWERMEQVSGGGGPAEFLSTHPSHDTRIDQLKEWMPEAMTIYQRRTA
                     MPAAPLPEIGGR"
BASE COUNT          385 a          440 c          521 g          392 t
ORIGIN      
        1 cggaaattct cttttcgaag tgtctccttg acggggaagc ttacacaggg accagatcat
       61 gtttacttag gacgacctca gtgcagagtc agatcagccc agccaagatg gatcgagtgg
      121 tcttgaagtg caatttggct accgagtcga gccgttctct tccatgtttc ttgatgatca
      181 tcttggcggc gagttcgaca gccggagagg cgcctttagg cagggtggtg ccgacttcgc
      241 cggaaagccg cttcagccgt atcaaaaatt ccgctcgtgc aagcacggat gccgcagcaa
      301 ctgctaagtc cgactcggct ttagtccgct gttccagcac aatctttcgt cccttttctt
      361 gcagtacgtt gagaatcagc ctttcgtctc cgaactgatc agagatggcc cgatcacagg
      421 tcactcgttc gagcaaggtt tcaagcgctt tggcatggcc ccaggcgagc aagcgattca
      481 gatttttgat cttggtatag agttcgttgt acttctgtgg cccgatggcg atgacgctgt
      541 gaggacaaat ggtcttaata tcgggagcca gttccaagat gcggctgtct gaaagtttct
      601 tgctgtcgcg gacctccatc agcttcaatt cgccttgcgt ggttgcatcc acaaacactg
      661 cggcaatgac gaggggccca aaataatcac ccttcccgga ctcgtcgatg ccgatacgtt
      721 cgatcctgct gcggggatgt gacgcagtca tcacgttcgt gctcctctgg aaatcccgtt
      781 acggcggcgg taatctatca accgaatcca tgttggtcaa tcgaacacga catatctatg
      841 cggcgttttt ggctggagga ttgaagcgat gcagactcca cgcgtgatgc gagcgacgac
      901 gatgtgttgt ctccttgccg gaggaattgg tttcgtaggg tgcgaaacca atccgtatac
      961 cggcaggtcg cagttgctga tgacgtcggt gagtcaagaa atgcaaatgg gggctcaggc
     1021 ctacaatcag gtcaagagtg atcccaaaat gaaaccttcc caagatccac gagagattga
     1081 accggtgaaa cgggtggcgg ctcggatcgt cgaagccgcg aagcggtcga agtatgcaga
     1141 aatggcccaa cagtttcaat gggaagtcac ggtcatcaag gatgataaaa cggcaaacgc
     1201 ctttgcgctg cctggtggga agatggcggt gtataccggc atcttcccca tggccaagac
     1261 ggaggccggc ttggcggccg tgatgggtca tgaagtcgtg catgccttgg ctcgccatgg
     1321 cgcggagcgc atgagtcagg ggcaggtcgt gaatatcggg cttcaagtcg tcggtgcggc
     1381 agtcgggata agcagcaaga atcctgcgct tggtcaggcc accatggcgg cgctcggagc
     1441 aggagcgcaa gtcggcgtgc tgcttccttt tagccgcaaa catgaatccg aggcagacca
     1501 tatcgggatt cttttggccg cagatgctgg gtatgacccg cgtgagtcgg ttgcactctg
     1561 ggaacggatg gagcaggtat cgggcggtgg aggcccggca gaattcttgt cgactcatcc
     1621 gagccacgac accaggattg atcaattgaa agagtggatg cctgaggcta tgaccatcta
     1681 tcagagaagg accgccatgc cggcggctcc tctaccggaa atcggtggca ggtaaggg
//
LOCUS       DATMTI010000151         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_5809, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000151 DATMTI010000000
VERSION     DATMTI010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5809"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VN039_02500"
     CDS             <1..997
                     /locus_tag="VN039_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44889.1"
                     /translation="ARAELPGKVFPINLANQLSVAPGEIKDYLINKEGDVVEKEEILA
                     ENKPLIKWFKTEIRSPVAGTIESVSLVTGQVLLREPPRVLQLLAYVDGTIIETIPQQG
                     VVVETACGLVQGIFGIGGETSGEITMAVKGPDEPLTPAHFTSTMKGKVVVGGSFLSSE
                     AMQQAKAVGVAGLVVGGIHDEDLRALLGYDLGVAITGTEHVGFTLILTEGFGTIPMAT
                     KTFKLLSTHVGRKASISGATQIRAGVIRPEIIIPRGEAAVGMNAQSQREGIHVGDPVR
                     IIRDPLFGRIGEVSALPPELTKISTESDVRVLEVTFSDGKRVVIPRTNIEVIEGA"
     gene            1071..2288
                     /locus_tag="VN039_02505"
     CDS             1071..2288
                     /locus_tag="VN039_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HWV44890.1"
                     /translation="MIFWAVSLSPSFTLAQAQISAPLELQVFDTPSDGGGSLTVQWAP
                     ASADGPDIRYQVLIGEASATDPTTFTIIAEFPANTKYVKDTKTAWWTRRSDKTWHQAI
                     IKSAKGVEVKDGTAYAVAVATVQGDRRGFSPVQVATSAANWFNWNQLNNLIIAVSFCG
                     LVFYTISHAKRNNIFLRRIPGLDAVDEAIGRATELGKPILYLTGAHDLHDPSTIAAAV
                     ILGKVAKRAALYETELMVPHREPITMAVCQEITKQAYLEAGKPDLFKEDANFFITADQ
                     FSYTAAVDGIMLRKKPAANFFMGHYFAESLLLTETGASTGAIQIAGTDADHQLPFFVT
                     TCDYTLIGEELYAASAYLSREPVQIGTLRGQDIGKAVILSMLAVGTVVATIGVVTGAQ
                     WPQIVLDLFRDLK"
     gene            2285..2935
                     /locus_tag="VN039_02510"
     CDS             2285..2935
                     /locus_tag="VN039_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44891.1"
                     /translation="MIFLRRQLPLLITLITGLLFAGQYYVPHPASEQMLTSATKWLQI
                     IGGFALVLGVTSLFHQHAVKIKRQEAGWGYSLVLYAGMLGTIAVGLWANGKESVDGAM
                     TAFGWVYNFMMVPLQGTMFAILAFFIASAAYRSFRARSREAAVLLVAAVIVMMGRVPL
                     GEYLIPLSGDVSSWILNVLNSSVRRAILIGVSLGAVALSFKIIFGVERSYLGGGKE"
     gene            3039..3869
                     /locus_tag="VN039_02515"
     CDS             3039..3869
                     /locus_tag="VN039_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44892.1"
                     /translation="MSFSERMLKIDRRIIFLVIGLCTLLPLLYPVGLPIKITSEVRGV
                     YDYIESLPERSVFLLSIDFDPASKPELHPQAIALLRHAFRKNLRVIAMTLWVSGTGMA
                     DQLVTQVAKEMGKEYGKDYVFLGWSPGGQAVIINMGQNLYSAFPSDYSGKPTKDLPIL
                     ADVRNLKDVTYMVSLGAGKPGVEEWYVFGKDKYKFEMGGGCTGVMAPGLYPLLRSGQI
                     NGLIGGLRGAAEYESLIGQKGKAVAGMDAQSATHVAIIVLVIMCNLFYFSLRQQRGQG
                     "
BASE COUNT          858 a          986 c         1175 g          879 t
ORIGIN      
        1 ggcccgagcc gaattaccag ggaaagtatt ccccatcaac ctggccaatc agctcagtgt
       61 ggcgcctggc gaaatcaagg attatctgat taataaggaa ggcgacgttg tcgagaagga
      121 ggagattctt gcagagaaca agccgttgat caaatggttc aagaccgaga ttcgatcgcc
      181 ggttgcagga acgattgaat cagtgtcgct ggtcacaggg caagtgctct tacgtgagcc
      241 tccgagagtc cttcagctct tggcctatgt ggacggaacg attatcgaaa cgattccgca
      301 gcaaggtgtc gtcgttgaaa cagcttgcgg tttggtgcaa ggcatcttcg gcatcggcgg
      361 agagacctcc ggcgaaatta caatggctgt gaagggaccg gacgaacccc ttactccggc
      421 tcatttcact tcaaccatga aggggaaagt cgtggtggga gggtcgtttc tctcgtccga
      481 ggccatgcag caggcaaagg cggttggtgt agcaggtctg gtcgtcggtg gaattcatga
      541 tgaagatctg cgggctttat tggggtatga cctcggcgtg gcgattacgg gaacagagca
      601 cgttgggttt acgctgattt taaccgaagg gttcgggacg attccgatgg cgaccaagac
      661 gttcaagttg ctctccaccc atgtcggtcg gaaggcctcg atttccggtg ccacccagat
      721 cagggccgga gttatccgtc cagaaattat cattccacga ggagaggcgg ccgtcgggat
      781 gaacgctcag tcacaacgag agggaattca cgttggcgat ccagtccgta tcatccgtga
      841 tcccttgttc ggacgcatcg gcgaagtctc ggcccttccg cccgagctta ccaagatttc
      901 cacagagagc gacgtgcgtg tgttggaagt gacgttttcc gacgggaaac gagtcgtcat
      961 cccgcggacc aatatcgaag tgatcgaagg ggcatgagcg gtgtgagggg tgaggggtga
     1021 ggaaaaggct gaagcgagga tgtccaagct ccgtctggtt ctcgttatca atgatctttt
     1081 gggcggtcag cttgtcgcca tccttcacat tggctcaagc ccaaatcagc gctcccctag
     1141 aactccaagt cttcgatacc ccgagcgatg gaggagggag cctcaccgta caatgggcgc
     1201 ctgcgtcggc ggatgggccg gacattcgtt atcaggtctt gattggagaa gcttctgcaa
     1261 ctgatccgac gacgttcacg atcatcgccg agtttcccgc caacaccaag tacgtcaaag
     1321 acacgaaaac tgcctggtgg accaggagat cggacaagac gtggcaccag gcgatcatta
     1381 agagtgcgaa gggcgtcgaa gtgaaggacg gtacggccta tgctgtcgca gtcgcgacag
     1441 tgcaaggaga ccgacgtgga ttcagccctg tacaagtcgc cacatcggcg gccaactggt
     1501 ttaactggaa tcagctcaac aatctgatca tcgccgtctc gttctgcggg ctggtcttct
     1561 acacgatcag tcacgccaag cggaataaca tttttcttcg tcgtattcct ggtctcgatg
     1621 cggtggacga agcgatcggg cgcgcgacgg aactgggcaa gccgatcctc tatctcaccg
     1681 gcgcacacga cctgcatgat ccctccacca tcgccgcagc ggtcatctta ggcaaggtgg
     1741 cgaaacgggc ggccctgtat gaaacggaac tgatggtgcc tcaccgggag ccgattacga
     1801 tggcggtctg tcaggagatt accaagcaag cctatttgga agcggggaag cccgatctct
     1861 tcaaggaaga cgccaacttt tttatcacgg ccgatcagtt cagctacaca gcggcggtgg
     1921 acggcatcat gctgcgaaag aagccagccg ccaacttctt catgggacat tactttgccg
     1981 agtcactgct gctcaccgaa accggcgcaa gtaccggtgc catacaaatc gctggaacag
     2041 atgcagacca ccaactcccg tttttcgtga cgacctgcga ctacaccttg atcggtgaag
     2101 agctctatgc cgcgagcgcg tacttgtcca gggaaccggt ccagatcgga acgctccgtg
     2161 gtcaagacat tgggaaggcc gtgatcttga gcatgctggc cgtcggaacg gtcgtggcga
     2221 cgatcggagt cgtcaccggc gcgcaatggc cgcagattgt tctcgatctc ttcagggatc
     2281 tcaaatgatc ttcctgcgtc ggcaacttcc actcttgatc accttgatta ccggtttgct
     2341 attcgcaggt cagtactatg tcccgcatcc ggcctcggaa cagatgctca cttctgccac
     2401 gaagtggctg cagatcatcg ggggattcgc attggtcttg ggtgtgacca gcctgtttca
     2461 tcaacatgcg gtcaagatca aacggcaaga agcaggatgg ggctatagcc ttgtcttgta
     2521 tgccggcatg cttgggacga ttgccgttgg actgtgggct aacggaaaag aaagcgtcga
     2581 tggcgccatg acggcgttcg gctgggtata caacttcatg atggtgccgc tgcaaggcac
     2641 catgttcgcc atcctggcct tcttcatcgc atcagccgcc taccgctcgt ttagagccag
     2701 aagtcgtgaa gcggcggtgc tcttggtggc ggccgtgatc gtgatgatgg gacgggtgcc
     2761 gctgggtgag tatctgattc ctctgagcgg cgatgtgtcg tcgtggattt tgaatgtact
     2821 taattcctcc gtgcgccgag ccattttgat cggggtcagc ctcggagccg tcgccttgtc
     2881 gtttaagatc atcttcggcg tggaacggtc gtaccttggc ggtgggaaag aatagcgtga
     2941 ggagtgaggc gtgaggggta aggcgtgtga gagtaagaca agatcgacgt agtttcgcct
     3001 aacgtttcac gcctaacgcc tcacggggtg gcagggttat gagcttttcg gaacgcatgc
     3061 tcaagatcga tcggaggatc attttcctgg tgatcggtct ctgtacgctc ttgccgttgc
     3121 tctatcctgt cggtttgccg atcaagatta cctcggaagt gcgcggggtc tacgactaca
     3181 ttgaatcatt accggagcgt tcggtctttc tcctgtcgat tgattttgac cctgcctcta
     3241 aacctgagct ccatccgcag gcgatcgcgt tgctgcggca cgccttcaga aaaaatcttc
     3301 gagtgatcgc catgacactg tgggtgtccg gtaccgggat ggccgatcag ttggtcacac
     3361 aagtcgcgaa agagatgggg aaagaatacg gaaaagacta tgtctttctc ggatggagtc
     3421 ccggaggcca agcggtgatc atcaatatgg gacagaatct ctattcggcc ttccccagcg
     3481 attacagcgg caagcctacg aaggatctgc cgattttggc tgacgtgcgg aatttaaaag
     3541 atgtcaccta catggtcagc ttgggagcgg gaaaaccagg cgtggaagag tggtacgtct
     3601 tcggcaagga caaatacaag tttgaaatgg gcggcggatg cacgggagtc atggctcctg
     3661 gactgtatcc tttgctaaga agcgggcaga tcaatggcct catcggcggt cttcgcgggg
     3721 cggcggaata tgaaagcctg atcggacaga aaggcaaggc cgtggccggt atggatgcgc
     3781 aatcggccac gcatgtggcg atcattgtgc tggtgatcat gtgcaatttg ttttattttt
     3841 ctctccggca gcagagggga caagggtaag gtcgaggttg aagggactcc tatgtcgt
//
LOCUS       DATMTI010000152         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_812161, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000152 DATMTI010000000
VERSION     DATMTI010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812161"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VN039_02520"
     CDS             complement(<1..420)
                     /locus_tag="VN039_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HWV44893.1"
                     /translation="MTQTIQIVLPEFQGFLVLISRIGGLLAALPVLSGRAVPVKVKVA
                     LVLALGVLLAPLVPLPAVPYDPVVLAAGLVNEMAIGLAIGLAVRLFFSALEVAGEMIG
                     VQMGFGVVQLFDPTTADHTPIIGQYSTLLATLIFLSLN"
     gene            complement(436..705)
                     /gene="fliQ"
                     /locus_tag="VN039_02525"
     CDS             complement(436..705)
                     /gene="fliQ"
                     /locus_tag="VN039_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HWV44894.1"
                     /translation="MTPEMVTELGRQALETTLLVSSPILGLSLFIGLAVSAFQAMTQL
                     NEATLTFVPKVVALFAALLLFLPWMLNVMTTYMANLLMNIPNYVH"
     gene            complement(768..1535)
                     /gene="fliP"
                     /locus_tag="VN039_02530"
     CDS             complement(768..1535)
                     /gene="fliP"
                     /locus_tag="VN039_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248836.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HWV44895.1"
                     /translation="MTDKHESRSRLLPVIIVGAMALLVMPSSEATAVGPSVSIDFGAG
                     GPKQTAVVVQILILLTVLSLAPALFIMVTSFTRIVIVLAFLRQALGTQTVPPNQVLLS
                     LALFLTMFIMAPVGQAVYNNALQPLMAEQISYEDAWKKGIEPVRNFMLRQVREKDLEL
                     FITLSRMPKPDRVEDVPTQAIIPAFILSELRIAFQIGFLIYIPFLIVDMVVASILMSM
                     GMMLLPPVVISLPFKLILFVLADGWYLVVGSMVRSFQ"
     gene            complement(1525..1929)
                     /locus_tag="VN039_02535"
     CDS             complement(1525..1929)
                     /locus_tag="VN039_02535"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HWV44896.1"
                     /translation="MIDLWESLFRTVSALAIVLVLMGIVAVTVRRLMGQRLGIAGGRP
                     LVRVLASSYIAPRKTIALVSVAGEYLIVGTTATDLVPLGRIGDSTELRELLATCEKPP
                     TETAPVPRDIFASWLRRLPLGVFHHDKGLHDR"
     gene            complement(1949..2278)
                     /gene="fliN"
                     /locus_tag="VN039_02540"
     CDS             complement(1949..2278)
                     /gene="fliN"
                     /locus_tag="VN039_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HWV44897.1"
                     /translation="MTGSQSPQPASFPTVQQAETGGTPKNIDFILDIPMSVTVYVGSA
                     KMAIRDLLQLAQGSVIELDKLAGEPMEVMVNNKLVARGEVVVVNEKFGIRLTDVVSAA
                     ERVQQLR"
BASE COUNT          540 a          683 c          654 g          433 t
ORIGIN      
        1 gttcagcgag agaaagatga gagtcgccag gagggtggag tattggccga tgatgggtgt
       61 gtgatcggcc gtcgtcggat cgaatagttg aaccacgccg aaccccatct gcacaccgat
      121 catctctccg gcaacttcca gcgcgctgaa aaataatcgg accgccagtc cgatcgccaa
      181 accgatggcc atttcattga caagcccggc cgccaagacg acaggatcat agggaacggc
      241 gggaagcggg accaagggag ccaacaggac tcccaacgcc agaacgagcg ccactttgac
      301 tttcacagga acagctcggc cgctcaagac cggcaatgcg gccaacagcc ccccgatacg
      361 ggaaatgagg accaagaacc cttggaattc tggaaggaca atctggatgg tctgcgtcaa
      421 cgccattgtc tcgtactaat gcacgtagtt ggggatattc atcagtagat tcgccatgta
      481 ggtggtcatg acattcagca tccagggaag aaagagcagc agcgccgcaa aaagggccac
      541 cactttcggg acaaaggtga gcgtggcctc gttcagttgg gtcatcgctt ggaacgcact
      601 gaccgccaga ccgatgaaca agctcagccc cagaatcggc gacgacacca acagcgtcgt
      661 ttccagcgct tgcctgccca gttccgtcac catttccggc gtcactcggt tctccttgct
      721 atcagtgatc agccgtcagt tgtcagctgc ccgcagacgg ctcaccgtta ctgaaaactc
      781 cgcaccattg aacccacaac caggtaccag ccgtcggcca atacgaataa gatcaatttg
      841 aacggcagag agatcacgac gggcggaagg agcatcattc ccatcgacat gagaatgctg
      901 gcgacgacca tgtcgacgat taaaaaagga atgtagatta agaatccgat ttggaaggca
      961 atccgtagtt cgctcaggat gaaggccggg atgatcgcct gggtcggcac gtcctcaacc
     1021 cgatccggtt tcggcatgcg gctcaatgtg ataaagagtt caaggtcctt ctctctcacc
     1081 tggcgcagca tgaaattccg caccggctcg atgcccttct tccatgcgtc ttcgtacgag
     1141 atctgttcgg ccattaacgg ttgcagcgca ttgttgtata cggcctggcc gaccggagcc
     1201 atgatgaaca tcgtcaagaa cagcgccaac gacagcaaca cttggttcgg aggaaccgtc
     1261 tgcgttccca gcgcttgccg aagaaaggcc agcacgatca cgatcctggt aaacgaggtc
     1321 accatgatga acaaggccgg cgccaaggag agcaccgtga ggaggatcaa gatctggacg
     1381 accacggcgg tttgcttcgg gccgcccgcg ccgaaatcga tgctcaccga cgggccgacc
     1441 gccgtcgctt ccgaagaggg catgacgagc agcgccatcg ctccgacgat gatcaccggc
     1501 aataggcggg agcgagactc gtgcttatcg gtcatgaagt cccttgtcgt gatgaaagac
     1561 gccgagcggt aggcggcgaa gccaggatgc aaagatgtcc cgcgggaccg gcgccgtctc
     1621 ggtgggtggc ttctcgcagg tggcgagtaa ttcccgtaat tcggtggagt cgccgatgcg
     1681 tcctaacggg acaagatcgg tcgcggtcgt ccccacgatt agatattctc ccgccaccga
     1741 cacgagggcg atcgtcttgc gcggggcgat gtagctgctg gccaagaccc ggacgagagg
     1801 acgcccgccg gcgatcccca aacgctgtcc catcaatcgc cggaccgtca ccgcgacgat
     1861 ccccatcaat accagcacga tggccaaggc cgagacggtg cggaacaggc tttcccacag
     1921 atcaatcacg gcctctccct cggatcattc agcgaagttg ctgcacgcgt tccgccgcgc
     1981 tgaccacgtc cgtcaagcga ataccgaatt tttcattgac gaccaccact tcgccgcgcg
     2041 cgaccagttt attgttcacc atcacttcca tcggctcgcc ggccagttta tccagctcga
     2101 tgacggagcc ttgggccagt tgcaacaggt cccgaatggc catcttggcg gatccgacat
     2161 acacggtgac gctcattgga atatccagga taaaatcgat gttcttcgga gttcctcccg
     2221 tctccgcctg ttggacggtc ggaaatgaag ccggttgcgg agattgactc ccggtcatct
     2281 gcgattcggc acgagttgcg gaatctggat
//
LOCUS       DATMTI010000153         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_727155, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000153 DATMTI010000000
VERSION     DATMTI010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727155"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="VN039_02545"
     CDS             complement(<1..145)
                     /locus_tag="VN039_02545"
                     /inference="COORDINATES: protein motif:HMM:NF023583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HWV44898.1"
                     /translation="MFEQAFKNIDDVLRREAGCTTELDYTEQTSWLLFLKYLDALEED
                     RATE"
     gene            complement(435..665)
                     /locus_tag="VN039_02550"
     CDS             complement(435..665)
                     /locus_tag="VN039_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HWV44899.1"
                     /translation="MPDSKIDFSDIPESTDEELRRARRVGRPASGAAKQLIAIRISPT
                     LLHQLRKMAAKRRKPYQTLIHELLEKAASRAA"
     gene            complement(1027..1287)
                     /locus_tag="VN039_02555"
     CDS             complement(1027..1287)
                     /locus_tag="VN039_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44900.1"
                     /translation="MRNTPPDVDERYRAMLMARPDEERLIMGCAMRDTAHALVEASLR
                     EQTPNATIRKGLFLRLYGHEFDAETRAKILAAIESAAIAVAK"
     gene            complement(1284..1454)
                     /locus_tag="VN039_02560"
     CDS             complement(1284..1454)
                     /locus_tag="VN039_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44901.1"
                     /translation="MEDSPTGGSCIGRRKAVCGCSDVRNLLSSVQDLDLADLDRWADR
                     LGLGALYQEVRP"
     gene            complement(1454..1789)
                     /locus_tag="VN039_02565"
     CDS             complement(1454..1789)
                     /locus_tag="VN039_02565"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HWV44902.1"
                     /translation="MQPTQISKADRPPDVEPTIRHVVPWRVVAVTAVSDFHLQVTFVD
                     GTRGEVDLRNFLNSADAVGTVFEPLRDPALFAQAQVVMGAVQWANGADLAPDAMYDAI
                     KAHGRWVVG"
     gene            complement(1906..2067)
                     /locus_tag="VN039_02570"
     CDS             complement(1906..2067)
                     /locus_tag="VN039_02570"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWV44903.1"
                     /translation="MAAKMFEVLSREGINIMMISTSEIKISCVIDEKYLELAMRSLHS
                     AFDLDQAQS"
BASE COUNT          448 a          599 c          562 g          475 t
ORIGIN      
        1 cctcggtcgc gcggtcttct tccagcgcgt cgagatattt caggaacagc aaccacgatg
       61 tctgctcggt gtagtcgagc tcggtggtgc agccggcctc gcggcgtagc acgtcgtcga
      121 tattcttgaa ggcttgttca aacattcgga tctccacgca gctgtaggaa aattagcgcc
      181 aagcttaccc gtttttctta gaagaaacta cgcacctatt ggggaagaag tgctgccctt
      241 cgccaagctt acgatgagac gctgagcgca gactggggag gacgaagagc cgaagctggc
      301 tgctctgatt aactgattgg tttcgtggat tggaccggaa catgcgagga gccaattgac
      361 tccttcaacc agcactgtgt gtttgctcgc cgcctgatct gccaagtttt gcgcaaggat
      421 gaattgccct ctcgctacgc tgcacgagac gcagcctttt cgagcaactc atggatcaag
      481 gtctggtagg gcttccgacg cttcgctgcc atcttcctca gctgatgaag cagcgtgggg
      541 gatatccgaa tggcaatcag ctgtttagcc gcgccgctgg ccggtctccc gacccgtcgt
      601 gcacgtcgaa gctcctcatc ggtggattcc gggatgtccg agaaatcaat cttcgagtcg
      661 ggcatatgcc tgacgctcct tgcgattcgc ctgtctcgcg ctaatgatac ggatcgtgtg
      721 ggtttcatga actttcctcc tcgtatagac gatgagtatg attcgtccgt tcagcgatcc
      781 ccccagccgt ttgaatcgga gttcttgctg ggaatgggca agatcctgcc aatccagcgc
      841 ggtacatctc taaagaccgt cgcggcttct tcaaatgaga caccatgctt ctcataattc
      901 gagatcgcct tcacgacatc ccaggtgaat acattcgtgt atatacatga aattgtccat
      961 catccgcaag aaccagacgt tgaaggcagc gcccacacct gcgagtattc gttgatagga
     1021 aatggcctac ttggcaacag caatggcagc agattcaatg gccgccagga tcttggcgcg
     1081 ggtttcagca tcgaactcgt gcccatagag gcggagaaac aggcctttcc ggatggtggc
     1141 gttcggcgtt tgttctcgta gagaggcttc cacgagcgca tgggcggtgt cacgcatggc
     1201 gcatcccatg atgagccgtt cttcgtcggg gcgggccatc aacatggccc gatagcgctc
     1261 gtcgacgtca ggcggtgtgt tcctcatggc cgtacctcct gatagagtgc gcccagtccc
     1321 aatcgatcgg cccaccgatc cagatccgca agatcgaggt cctgcaccga gctgagcaga
     1381 ttgcggacat cgctgcaccc gcaaacggct ttccttcgcc cgatgcaaga cccaccggtt
     1441 ggtgaatctt ccatcaccca acaacccacc ggccatgggc tttgatcgcg tcgtacatgg
     1501 catcgggcgc aaggtccgct ccgttcgccc attgcaccgc tcccatgacc acttgagcct
     1561 gagcaaagag agccggatcg cgtagcggct caaacaccgt accgacggcg tcggcactat
     1621 taagaaagtt ccgcaggtct acttcccctc tcgtaccatc cacaaatgtc acctgtaagt
     1681 gaaagtcaga tacagccgtc accgccacca cccgccaagg cacgacatgg cgaattgttg
     1741 gctctacgtc aggggggcga tcggctttgg agatttgagt tggctgcata aattccagtc
     1801 ctccatcaat tcatctcgat gttcacttgg ctggggagga accccaaccc tacgcgctaa
     1861 gaagcgggag atgcttgtcg accgtcagat ttcggcctga ccatttcagg actgagcctg
     1921 atcgagatcg aatgccgaat ggagggatcg catggccagt tcgagatatt tctcatcgat
     1981 gacgcaggag attttgatct ccgaggtgct gatcatcatg atgttgattc cttcacggga
     2041 caacacctcg aacattttcg ccgccacacc tgaatgcgag cgca
//
LOCUS       DATMTI010000154         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_684534, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000154 DATMTI010000000
VERSION     DATMTI010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684534"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VN039_02575"
     CDS             complement(<1..627)
                     /locus_tag="VN039_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV44904.1"
                     /translation="MDESSRLTKTKEQWARARRGGEEREVFYEGDDRLPPGQHLVDNF
                     PVLDLGFKPEIPLCEWRLSIGGFVANPLVLTWEQFMAQPPFKDRSDFHCVTSWSRYDN
                     DWEGVSFKHLISIAKPLSLARFVLFKSYDDYTTNLPFEACDDGDVLLAHSWNGKPLSR
                     DHGGPVRVIVPKRYGWKGAKWVKDITFSDKDEKGFWEVRGYSNTAFPWK"
     gene            846..1583
                     /locus_tag="VN039_02580"
     CDS             846..1583
                     /locus_tag="VN039_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HWV44905.1"
                     /translation="MIRHDAPMMTPISNQHESSDSNFGKISVAVGIALAIGAFFYFDL
                     GRFLSLTALKENRDNLLVFADANFAAAVGIFIAVYAIVTGLSLPGAVILTLAGGFLFG
                     AIWATLFVNIGATVGATLAFLTARYLLRDTVEQKLGKWLGPFQEGFTKNAFSYLLTLR
                     LIPLFPFFVVNLVSGLTRVNVGTYVVATVIGIIPGSFVYAYAGRQLGTINSLKEIASP
                     NVIGAFVLLGLLALGPVVYKRIAAKPA"
     gene            1663..>2812
                     /locus_tag="VN039_02585"
     CDS             1663..>2812
                     /locus_tag="VN039_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV44906.1"
                     /translation="MSEHNQSLILPNDEYNQELVANVHPPDWVNPEPTGRYNIVVIGA
                     GTAGLVTAAIAAGLGAKVALIEKHLMGGDCLNVGCVPSKGVIRAARAWGDLRKATEFG
                     LHIPAGVKYDFGAVMARMRKLRARISRNDSAQRYMKLGVDVYIGSGRFLGFDTVQVEG
                     PAGDRILTFAKAAVCTGARAAIPDTPGLQEAGYLTNETIFSLTELPQRLGVIGAGPIG
                     CELAQSFARFGSQVYLIEATHGILPNEDRDAAGIVEQQMFRDGVKLLCCGKYLKVQKT
                     EGGKRLTVNSHGQHYDVLVDEILVAVGRIPNIERMGLETAGVSYDKSGIRVNARLQSS
                     NPKIFAAGDVCSHYKFTHAADAMAQIVIQNALFPHPLGLGYASIESLNM"
BASE COUNT          604 a          761 c          797 g          650 t
ORIGIN      
        1 cttccatggg aacgcggtgt tggaataccc gcggacttcc caaaacccct tctcatcctt
       61 atctgaaaac gtaatgtcct tcacccactt cgcccctttc cagccataac gtttcgggac
      121 gatcacacgt accggcccac catggtctcg ggataggggt ttgccgttcc agctatgggc
      181 cagcagcaca tcgccgtcgt cacaagcctc aaacgggagg ttcgtggtgt agtcgtcata
      241 ggacttgaac aagacaaatc gggcaagcga cagcggtttc gcgatggaaa tgaggtgttt
      301 gaaactcacg ccttcccagt cattgtcata acggctccat gaggtgacgc aatggaagtc
      361 cgaccggtct ttgaatgggg gttgtgccat gaattgctcc catgtcaaga caagcgggtt
      421 agcgacaaat ccgccgatgc tgaggcgcca ttcgcataat gggatctcag gcttgaaccc
      481 gaggtccagt acaggaaagt tatcgacgag gtgctggcct ggaggcaggc gatcatcgcc
      541 ttcatagaag acttctcgtt cttctcctcc tcgccgagcc cgggcccatt gttctttggt
      601 ctttgtcagt ctgcttgatt catccattcc gtctccgccc tccctgcaca cagaatatgg
      661 gaagtgaagc cgatctggca acccctgacc ggtggtcgga gaagtccgac aacgaatatt
      721 tcatcgagca gcagcctccg taggcacttt tggattgccg ctcggccatg tcaggcttcc
      781 tggtccaggc ggacagacag gcaagcaagc agcctatctc attgacagaa cacagaggag
      841 ccaccatgat acgccacgac gctcctatga tgaccccgat ctcgaatcag catgagtcca
      901 gtgattcaaa cttcggcaag atatccgtcg cggtcggtat cgctttggcc atcggcgcgt
      961 ttttctattt cgatctcggg cggttcttat cgctaaccgc actgaaagaa aatcgggaca
     1021 acttgttggt atttgcagat gccaactttg cggcggcggt gggaatcttc atcgcggtct
     1081 atgcgattgt gacaggattg tccctgccgg gggccgtcat tctcaccttg gccggagggt
     1141 tcttgttcgg cgccatatgg gcaacgttgt ttgtgaatat cggagcgacg gtaggagcga
     1201 cccttgcctt tctcaccgct cggtatttgc tgagggatac ggtcgagcag aagttaggga
     1261 agtggttggg accattccaa gaagggttta ccaagaacgc attcagctac ttgttgacct
     1321 tgcgactcat cccacttttc cccttcttcg tggtgaacct tgtgtccggg ctcacgcgcg
     1381 tgaacgtcgg gacatatgtc gtcgccacgg tgattggaat cattcccggt tcattcgtct
     1441 atgcctatgc gggacgccaa ctgggtacga tcaactcgtt gaaagaaatc gcatctccga
     1501 acgtcatcgg ggccttcgta ctgctaggac tgctggcgct gggaccggtt gtctataagc
     1561 ggattgcagc gaaaccggcg tgatggccat ctcatcgatt gacgacgcac agcgaaggat
     1621 gtcaggctat gatcacttaa cccatctcaa ggggtcattg gaatgagtga gcacaatcaa
     1681 tctctgatcc ttccgaacga cgaatataat caagagctcg tcgccaatgt gcatcccccc
     1741 gattgggtca atccagagcc caccggccgc tacaacatcg tcgtgattgg ggcggggact
     1801 gccgggctgg tcacggccgc cattgccgcg ggccttggag caaaggtcgc cttgatcgaa
     1861 aagcatctga tgggcggcga ctgtctcaat gtcgggtgtg tgccgtcgaa aggagtgatt
     1921 cgagcagcca gagcctgggg cgacttacgg aaagccacag agttcggtct tcatattccc
     1981 gccggtgtga agtatgactt cggtgctgtg atggcacgca tgaggaaact gcgggcccgc
     2041 atcagccgga atgactccgc ccaacgatat atgaaactcg gcgtagacgt gtatatcggc
     2101 agcggacggt ttctcggttt tgatacggtc caagtggaag gtccggccgg tgatcgaatt
     2161 ctcactttcg cgaaggccgc tgtctgtacc ggtgcgaggg cggccatacc cgatacgccc
     2221 ggtcttcagg aagccggtta tctaacgaac gaaaccattt tttctttgac cgagctgccg
     2281 cagcgtctgg gggtcatcgg ggccggacct atcggctgcg agctggcgca gtccttcgcc
     2341 cgttttggga gccaagtcta tctgattgaa gcgacgcacg gcatcctgcc caatgaggat
     2401 cgagatgcag ccggtatcgt tgaacagcag atgtttcgcg atggtgtgaa gctgctctgc
     2461 tgtggaaaat acttgaaggt gcaaaaaacc gaaggcggga aacgtctgac ggtcaattcg
     2521 catggccaac attacgatgt cctcgtcgac gagattctcg tcgcggtcgg tcggattccg
     2581 aacatagaga ggatgggttt ggagaccgcg ggagtctcgt acgataagag cgggatcagg
     2641 gtgaatgccc gattgcagtc gtccaacccc aagattttcg cggccggtga tgtctgttca
     2701 cactataagt tcacacacgc cgctgatgcc atggcacaaa tcgtcatcca aaacgcgttg
     2761 ttccctcacc ccttgggctt gggctacgcc agcatcgagt cgttgaacat gc
//
LOCUS       DATMTI010000155         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_260248, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000155 DATMTI010000000
VERSION     DATMTI010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260248"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VN039_02590"
     CDS             <1..752
                     /locus_tag="VN039_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44907.1"
                     /translation="SQTGWNSKKFLFDKINPIGYNHNSRFTAGTVFKSRQSRSLIMSE
                     VIESTATPKAKTEYTEVAMDDGRKVKFAGKRQVDKTVVTDEDAGNVTVRFDFRNGRTL
                     SISSEDLPDQTCLAALGHGISQKCGDEYAGVKDVDDMVLAVEDMIARLTRGDWSAPRE
                     SSDSFSGASVVIRAICEVTGKSVEEVKAFLQGKIDEAEKRGEKLSRNELYRSFRAASS
                     KTGQVIARLEQEALAKSSKVSAADLLDELNG"
     gene            949..1224
                     /locus_tag="VN039_02595"
     CDS             949..1224
                     /locus_tag="VN039_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44908.1"
                     /translation="METEMFLGGIPLLVAYTYDPGRPASGPYAPEYPTLEVENVSEML
                     SDECTSLLDLIEEVGGLGSFKRHLLVHLEKTGELRGEGKSWPERSKT"
     gene            1280..1519
                     /locus_tag="VN039_02600"
     CDS             1280..1519
                     /locus_tag="VN039_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44909.1"
                     /translation="MSAQNTGGPAFPTIEGCYEQKYAGEGMSLRDYFAAKSLQGCMAY
                     FNESRGDFHTNAQPDEVAKYCYEVADAMLFARDQS"
     gene            1651..1863
                     /locus_tag="VN039_02605"
     CDS             1651..1863
                     /locus_tag="VN039_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44910.1"
                     /translation="MSESMSLAEAFPLEQARVRVVLGHYKEIGPAGMFGAAFIEQDLR
                     EADAAVASGDVIRMMRSYAKLKDIES"
     gene            1860..2330
                     /locus_tag="VN039_02610"
     CDS             1860..2330
                     /locus_tag="VN039_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44911.1"
                     /translation="MTAAIKHCPQPDPPELVMRADGYHYRTEDEMNMQALVDGLSAQW
                     QRERAETQLTLGKLIAALEAMPSGACVANLCNPASYRGYYTDLYFEEGHGTRLASGLL
                     SDCKAAMGQVYTGYKGGEYVMGALTPLWVATYGSCGKKLMAVHEGGGLETAEDE"
     gene            2327..3064
                     /locus_tag="VN039_02615"
     CDS             2327..3064
                     /locus_tag="VN039_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44912.1"
                     /translation="MTQDTQRKLELLQQAHDRARAEVMRLAERLRKYEPGHSMHLNAA
                     PADRDVMRQAPGGWKLVPIKPSAEMLDTGCAARHGLAQAIYEDMLSAAPTPPAEQPID
                     MVLHCPSCGKQHIDAPESELTAYADGHETVWRNPSHRSHLCHGCGHIWRPADVATNGV
                     QAIKTKGKNDSASAEQQGEQEPNSVVDTTLKNLRRLLEHHKTFQARVADLRAKVVALL
                     ESGPEDEPEMVLRSDVLAIFNKAGGDV"
     gene            3061..3405
                     /locus_tag="VN039_02620"
     CDS             3061..3405
                     /locus_tag="VN039_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44913.1"
                     /translation="MKRYTMGIFGGVPAMFEQLDGGYVRWRDVASLLTICGQSDTPID
                     PDLVGCGQPILRVDEVYRCTDCGVPFHKDCAKKHFETDTPEHSAKVYEEQIRRLDKND
                     VLAEIDKMGGGV"
     gene            3465..>3664
                     /locus_tag="VN039_02625"
     CDS             3465..>3664
                     /locus_tag="VN039_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44914.1"
                     /translation="MTRPKEWAPSLRVNISPIPRQPAPLGLAELMRRLQFEDQVLDNK
                     HRMAFLSQLRDDARVVITAIAQ"
BASE COUNT          902 a          959 c         1090 g          713 t
ORIGIN      
        1 tgagccagac gggctggaat tctaaaaaat tcctatttga caaaatcaac ccgattgggt
       61 ataatcacaa ttcacggttc acggcgggca ccgttttcaa gtcccgccaa tcaaggagtc
      121 tcattatgag cgaagttatc gaaagtacgg ctacgccgaa ggcaaagacg gagtacacag
      181 aagtagccat ggatgatggc cggaaggtta agtttgcggg gaaaaggcag gttgacaaga
      241 ctgtcgtgac ggatgaagac gcgggaaacg ttactgttcg cttcgacttc cggaatgggc
      301 ggactctgtc tatttcctcg gaagatctgc ccgatcaaac ctgcctcgca gcccttggcc
      361 acggaatctc gcagaagtgc ggggacgaat acgccggtgt aaaggacgtg gatgatatgg
      421 tcctggccgt tgaggacatg attgctcgcc tgacccgagg tgactggtcc gctccgcgcg
      481 aatcttcgga ttcgttctct ggtgcgtctg tcgtcatccg ggcgatttgc gaagttaccg
      541 gcaagtccgt ggaagaagtc aaagcattcc tccaagggaa gatcgacgag gcagagaagc
      601 gtggagagaa gctttcccgg aacgaactct atcgttcctt ccgcgcggca tcctcgaaaa
      661 ctggccaagt catcgcccgt ctcgaacagg aagctctggc gaaatcttcc aaggtttccg
      721 cagccgatct cctggacgaa ctgaacgggt aaggagagcc ctaaaggtag ggaatccgcg
      781 agggttcccc tcctctaggc cttttccgca acggagaagg gccgagttgg tctggatgaa
      841 ttgaacctcc accagactgg cgccaccccg gggaagggaa gtttctttgt ctccttcttc
      901 ccttcctcgg caagtggttt tcttcggggg ttcattagga ggtttatcat ggaaactgaa
      961 atgttcctgg gcgggattcc gctccttgtg gcctatacct acgacccagg aaggcctgca
     1021 tctggtccct acgcgccaga atatcccacg ctggaagtgg aaaatgtttc ggagatgctt
     1081 agcgatgaat gcacctctct ccttgaccta atcgaagagg tcggaggctt gggttccttc
     1141 aaaaggcatc tactcgttca cctggaaaag actggggaac tgcgaggaga aggaaaatcg
     1201 tggcctgaga ggagcaagac atgatggagc tggcagacga ccgttcaaag ctgctttgca
     1261 tcgaatcggg gctgacgcca tgagcgcgca gaacacgggc ggcccggcgt ttccgacgat
     1321 tgagggatgt tatgagcaga aatacgcagg agagggtatg tccctgcgcg actacttcgc
     1381 ggcgaagtcc ttgcagggat gtatggctta tttcaacgag tcccgcggtg attttcacac
     1441 caacgcacaa cccgacgaag tggcgaaata ttgctacgaa gtggccgacg ccatgctttt
     1501 cgcaagggac caatcatgag cactcaagcc acaaatatgc acccgacctt aggcgtgcca
     1561 atgacagaga cgttgcagaa caacgtgcat caggtctact ttcgcaccgg cttgttagcg
     1621 gggccaccaa caatactgta ggagatttga atgagcgaat caatgtcact tgccgaggcg
     1681 ttccccctgg agcaggcccg cgtgagggta gttctaggtc attacaagga gatcggaccc
     1741 gccgggatgt ttggtgccgc cttcattgag caagatttac gcgaagccga tgcggctgtg
     1801 gcaagcggcg atgtcatccg catgatgaga tcttacgcga aactgaaaga cattgaatca
     1861 tgaccgccgc catcaagcac tgcccgcagc cagacccacc cgagctcgtc atgagagcgg
     1921 atggatacca ctatcgaacg gaggatgaga tgaacatgca ggcactcgtt gacgggcttt
     1981 ccgcccaatg gcagcgcgag cgagcggaaa cgcagctcac gcttggcaag ctaatcgcag
     2041 cgctggaggc aatgcctagc ggcgcctgcg tagcgaatct ttgcaatcca gccagctacc
     2101 gaggctatta cacagacctt tactttgaag aaggccacgg cactcgcctg gcgtctggtt
     2161 tgctcagcga ttgcaaagct gcaatgggcc aagtctacac gggctacaag ggcggcgaat
     2221 acgtgatggg cgcactaacc ccgttgtggg tggcaacgta cggaagttgc ggaaaaaaat
     2281 tgatggccgt acacgaagga ggcggcctgg aaacagccga agacgaatga cacaagacac
     2341 acagcgcaaa ctggaactgc tgcaacaggc ccatgaccgc gcacgcgcgg aagtgatgag
     2401 gcttgctgaa aggctcagga aatacgagcc ggggcacagc atgcacttga atgcagcacc
     2461 ggctgaccgg gatgtgatgc ggcaggcgcc cggagggtgg aagctggtgc cgattaaacc
     2521 aagcgcggaa atgctcgata ctggatgtgc ggctcgacac ggcctcgcgc aagccatcta
     2581 cgaagacatg ctctctgccg cacccacgcc accggccgag cagcccatcg acatggtgct
     2641 gcactgcccg tcgtgcggaa agcagcacat cgacgcgcca gaatcggaac tcactgccta
     2701 tgccgatggg catgaaaccg tgtggaggaa tccatctcac cggtcgcacc tgtgccacgg
     2761 ttgcggccac atctggcgcc ccgctgacgt ggccaccaac ggcgtgcagg cgatcaagac
     2821 caaggggaag aacgactcgg catcagccga gcagcaggga gaacaggagc caaactcagt
     2881 tgtagatacc acactcaaga atttgaggcg attgctagaa caccataaaa cattccaagc
     2941 ccgagtggcc gatctgcggg ccaaggtggt ggcgctgctg gagtcaggcc cagaggacga
     3001 gccagagatg gttctccgtt ctgacgttct ggcgatattc aacaaggctg gaggcgacgt
     3061 atgaagcgct acacgatggg aattttcggc ggcgttccag ccatgtttga gcagctagac
     3121 ggaggttatg ttcgatggcg cgatgtggca tcactgttga ctatctgcgg gcagtctgac
     3181 acaccaatag acccggactt ggttggatgt ggtcagccga ttctgcgtgt cgatgaagta
     3241 tatcgatgca cggattgtgg tgtaccgttt cacaaggact gcgctaaaaa acattttgaa
     3301 acggacacgc cagagcattc agccaaggtg tatgaagagc agattcgtcg tctcgataaa
     3361 aacgacgtgc tggcagaaat cgacaagatg ggaggcgggg tatgagcgag caaatacgtt
     3421 gcacatattg cggtgaagtc ggccactcgg ttgacaactg ctgcatgact cgtccgaaag
     3481 agtgggcgcc cagtttgagg gtaaacatca gcccaatccc acgccagccc gcaccactgg
     3541 gattggcgga attgatgcgc aggctccagt tcgaggatca agtcctggac aataagcaca
     3601 ggatggcgtt tttgagccag ttgcgcgacg acgccagagt agtcatcacc gccatcgcgc
     3661 agga
//
LOCUS       DATMTI010000156         4196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_557746, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000156 DATMTI010000000
VERSION     DATMTI010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557746"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2278)
                     /gene="rpoB"
                     /locus_tag="VN039_02630"
     CDS             complement(<1..2278)
                     /gene="rpoB"
                     /locus_tag="VN039_02630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWV44915.1"
                     /translation="MSETTLQEFVERKDFSRIRTNIDIPDLIEIQKRSYEEFLQFEVE
                     SERRKDHGLQAALASVFPIPDYNNTAVLEFSSYTLGTPKYDERECLEQGMTFAVPLKL
                     RVRLVVLDKEDKGPRKKVLDVREQEVYVGELPLMTERGTFIVNGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FGFSSDDLLKMYYPVEEIRVSKGKMFRKLDPEIHHGLRCSAEVTDKGGKEPLVREGAR
                     LTKGLIAKLKASGVKEIPLLPTELVGRAILTELVDSKKNRLAEKNQRLTAEMVEQIAE
                     SDVEEFKVIYLDTATATPVILDTLEMEKIGSKEEAMVEIYRRLRPGETPSVETARALF
                     DNLFLNSKRYDLSPVGRLKLNKKLGLDLPLEQRTLTAQDIVEVIRYLVNLKIGKGEID
                     DIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVAA
                     VKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRIC
                     PIETPEGPNIGLITSLATYARINQFGFIEAPYRKVVKGRVTDEIEFLSAIEGDKYIIA
                     QANSKLDGAGKLVSETVSCRHGGDFVLAAPDKIEYMDVSPKQVVSVATALVPFLEHDD
                     ANRALMGSNMQRQAVPLVTSEAPLVGTGMEAVVARDSGYVIQARRAGVVESVDATRIV
                     VRADSKDGKRGKDSGLDVY"
     gene            complement(2404..2802)
                     /gene="rplL"
                     /locus_tag="VN039_02635"
     CDS             complement(2404..2802)
                     /gene="rplL"
                     /locus_tag="VN039_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWV44916.1"
                     /translation="MPATEGKLSQEELIKAIESMSVLDLAELVKGLEARFGVTAAAPV
                     AVAAAPAAGGAAAPAEEKTAFDVVLASAPADKKIQIIKVVRELTSLGLKEAKDLVEGA
                     PKPVKTGVAKEEADTMKKKLEESGAKVEIK"
     gene            complement(2830..3336)
                     /gene="rplJ"
                     /locus_tag="VN039_02640"
                     /pseudo
     CDS             complement(2830..3336)
                     /gene="rplJ"
                     /locus_tag="VN039_02640"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            complement(3423..4115)
                     /gene="rplA"
                     /locus_tag="VN039_02645"
     CDS             complement(3423..4115)
                     /gene="rplA"
                     /locus_tag="VN039_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWV44917.1"
                     /translation="MGKKMDAAIKNVEPRVYGLREAVETVKRSAYAKFDESVDLALRL
                     GIDPKRSDQLVRGTASLPHGTGKKVRVLVFAKGEKEQEARQAGADYVGADDLMEKIKG
                     GWMDFDCAISTPDLMASVGKLGKVLGPRGLMPNPKTGTVTFEVGKAVADIRKGRVEFK
                     VEKAGIVHVPVGKVSFDPAKLYDNASAILESVIKAKPASCKGRYLKSATIASTMGPGV
                     KLDTIALTKQWS"
BASE COUNT          848 a         1362 c          999 g          987 t
ORIGIN      
        1 catagacgtc cagcccagaa tcctttcccc tcttgccgtc ttttgaatcg gcccgcacca
       61 cgatgcgggt ggcatcgacg ctttccacaa ccccggctcg ccgagcctgg atgacatacc
      121 cggaatctcg cgccaccacc gcttccatcc ctgtcccaac cagaggggct tccgacgtca
      181 ccaagggaac cgcctggcgc tgcatgttgg aacccatcag cgcacggttg gcatcgtcgt
      241 gttccaagaa cggcacgagc gcggtcgcga cacttactac ttgcttcggt gacacgtcca
      301 tgtactcaat cttatccgga gcagccagga caaaatctcc accgtggcga caggagacgg
      361 tttccgatac caacttgccc gcgccatcca attttgagtt ggcctgagcg atgatgtatt
      421 tgtcgccctc gatcgccgag agaaactcta tttcatccgt cacgcgtccc ttgacgactt
      481 tccgatatgg tgcctcaatg aatccgaact gattgattcg tgcgtaggtc gccagcgaag
      541 taatcaatcc gatattggga ccttccggcg tctcgatcgg gcaaatccga ctgtagtgag
      601 acggatgaac gtctcgaact tcgaacccgg ccctctctct cgtgagtccg cccggaccaa
      661 gagccgacag gcgccgttta tgcgtaattt cagccagcgg gttcgtttgg tccatgaatt
      721 gagacagctg actgctgctg aaaaactcct tgacggctgc gacaaccggc ttggcattga
      781 tcaagtcatg cggcagcacc gtttccatat caagaagatt catgcgctcc ttgatgcttc
      841 gctccatccg caccaagccc agccggaatt gattttcaag gagttcaccg acggatcgca
      901 cgcggcgatt acccaagtga tcaatatcgt cgatttcccc tttgccgatc ttcagattga
      961 ccaggtagcg gatgacttcc acaatgtcct gagcagtcag ggtgcgctgc tcgagcggca
     1021 agtccaaacc aagcttttta ttcagcttga gccggccgac cggagataaa tcgtagcgct
     1081 tggaattcag aaatagattg tcgaacaatg ctctcgccgt ctcgactgac ggcgtctcac
     1141 cgggacggag gcgacggtag atttcgacca tcgcttcctc cttcgacccg attttctcca
     1201 tctccaaggt gtcaagaatt accggcgtcg cggtcgccgt atccaggtaa atgaccttga
     1261 attcttccac atcgctttca gcgatttgtt cgaccatctc ggccgtcaat cgttggtttt
     1321 tctccgctaa cctgttcttc ttcgaatcga ccaattccgt gagaatggcg cggcccacca
     1381 attccgtggg cagtaaggga atctccttca ctcccgaggc cttcagcttg gcaatcaggc
     1441 ctttcgtcag cctcgccccc tcccgcacca acggttcttt gccgcccttg tccgtgacct
     1501 cggcggaaca ccgaagtccg tggtggatct cgggatccag cttgcggaac atcttcccct
     1561 tcgacacgcg aatttcttcc acggggtagt acatcttcag caagtcgtcg ctcgagaaac
     1621 cgaaggcctt caacagaatg gtggccggca ttttccgacg gcgatcgatc cgcacatata
     1681 gaatatccct ggcatcgaac tcgaaatcga gccatgagcc ccgataggga ataattctgg
     1741 ccgagtacaa caccttgccg ctcgcatggg tccgaccttt gtcatgtgtg aacgacgcac
     1801 ccggtgaccg gtgaagctgg ctgacgacga ctcgctcggt cccgttgacg ataaaagtcc
     1861 cccgctcggt catgagcggc aattcaccga catagacctc ctgctcacgc acatcgagca
     1921 ctttcttgcg aggcccctta tcctctttat cgagcaccac caggcgaaca cgcagcttca
     1981 atggaaccgc gaaggtcata ccctgctcaa gacattcgcg ctcgtcgtac tttggcgtcc
     2041 ctaacgtata gctcgaaaat tcgagcacgg ccgtgttgtt atagtccgga atcggaaaca
     2101 cgctggccaa cgccgcctgc aatccatgat ccttccgacg ctccgattcg acctcaaact
     2161 gcaaaaactc ttcgtaggag cgcttctgga tttcgatcag atccgggata tcgatgttgg
     2221 ttcgaatgcg agaaaaatct ttccgctcga cgaattcttg aagcgtcgtt tcggacattc
     2281 ctactcctcc acgctggttg gcggtgaaca gctgtcacct gtcacgacag attcccgaaa
     2341 taccgataaa cctcggcctt cagcgaatac cgaaagagcc gcgacgatga cgaactcgct
     2401 tccttacttg atttcgacct tggcaccgct ttcttcgagc ttcttcttca tcgtatcagc
     2461 ctcttccttg gccaccccgg tcttgaccgg tttcggcgcg ccctccacga ggtccttggc
     2521 ttctttcagg ccgagactcg tcagctcccg caccaccttg atgatctgga ttttcttgtc
     2581 ggccggcgcc gacgcgagaa cgacgtcgaa tgccgtcttc tcttcggccg gagcagctgc
     2641 gccgccagcc gccggagccg cagccaccgc aaccggcgcc gccgccgtca cgccgaaccg
     2701 cgcctcaagc cccttcacca attcggccag atcgagcacg ctcatgcttt cgatcgcctt
     2761 gatcaattcc tcttgcgata attttccttc cgtagccggc atgtcccctt ctcctttccg
     2821 tttatcttga atggctgcaa tgactcgcac aaacttcgat acgaccgcgt tcaacgtata
     2881 gacaacgcca cgaatcggcc cctgcatggc cgaaagcaac atggcaacga gcacttcctt
     2941 tttcggcaac ctcgcaacgg cggccagttc ggccggctgg aggattttgc cttccagcac
     3001 cccggccgtc atccgaatct tctcttcacg cttctccgcg ccgataaatt ctttgagcac
     3061 ctttgtcggc aacaccggat cgtcatagcc gatgactacc ccggtcggcc ccttgagatg
     3121 cactttgagt ccggccaata tcgtcccctc ggcggcacgc gccgcgagcg tattcttcac
     3181 aattcgatat tctgcctttg ccccgcggag ctgtttgcgt aactcagtga tctggttgac
     3241 cggaaggccg acgcattccg tcaaaatagc cagccgcgca cgaccgaatt tctcggtcaa
     3301 ctccgccact gccgtaacct tctcttcctt cttcatcgct ccctctctcg tcgctaatcg
     3361 ttatccgttc aacgtcaccc gtccggcgag ttgcgatgaa cgattcacgt cgaatgcgtt
     3421 tttcagctcc actgcttcgt cagcgcaatg gtatccagtt tcacaccggg tcccatcgta
     3481 cttgcaatcg tggcactctt gagataacgc cccttgcatg aggcgggttt cgccttgatg
     3541 acggattcaa ggatggccga tgcattgtca tacagcttcg cgggatcgaa cgagactttt
     3601 cccaccggca catggacgat cccggccttc tcgaccttga actcgacacg cccctttcgt
     3661 atatcggcaa cggccttgcc gacttcgaag gtgaccgtgc cggtcttagg attcggcatc
     3721 agcccccgag gcccgagtac cttgcccagt tttccgaccg aggccataag gtccggcgtg
     3781 gaaatcgcgc aatcgaaatc catccatcca cccttgattt tttccatcaa gtcatcagcc
     3841 ccgacatagt cggcacccgc ttgccgcgcc tcctgctcct tttcaccttt ggcaaacacc
     3901 aggacacgaa cctttttccc ggttccgtgt ggaagcgaag ccgtccctcg aaccagttga
     3961 tccgagcgct tcggatcgat tcccagcctg agcgcaagat cgaccgattc gtcaaacttc
     4021 gcataggcag accgcttcac ggtttcgacc gcctcacgca gcccatagac gcgcggctcg
     4081 acattcttga tcgccgcatc cattttcttt cccataacca gacgctcctt cgatcccgag
     4141 attagccttg aatgacgacg cccatgctac gggcggtccc ttcgatgatt tttgcc
//
LOCUS       DATMTI010000157         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_48257, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000157 DATMTI010000000
VERSION     DATMTI010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48257"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..357)
                     /locus_tag="VN039_02650"
     CDS             complement(88..357)
                     /locus_tag="VN039_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44918.1"
                     /translation="MTDAQVEEIKKHFGVVAEALQSNIRQIAEGHASIRSELQEIRDE
                     FRDEFKEMRALMRLSFSQLDQRIRTLETDISTLKARMDRLETHRA"
     gene            complement(423..593)
                     /locus_tag="VN039_02655"
     CDS             complement(423..593)
                     /locus_tag="VN039_02655"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWV44919.1"
                     /translation="MPISRELDGLRKYRARRFRIIYAVHQKTRIIRLMAVGHRRSVYE
                     ELTKRFRRKSSE"
BASE COUNT          417 a          413 c          463 g          409 t
ORIGIN      
        1 actaaagagg acaagtgcga gtgtgcagag aatgagcgcc cgaaatctca ccatgtcaca
       61 tctcgcgtaa agaatagccg gcaaaagtta ggcacggtgg gtttcgagac gatccatcct
      121 ggctttgagc gtggagatgt ccgtttcgag tgtgcggatt cgttgatcga gctgtgagaa
      181 cgagagtcgc atgagggcgc gcatttcttt gaattcatct cggaattcgt cccggatctc
      241 ctgtaattca ctcctaatgc tcgcatgccc ttcagcgatc tggcggatgt tactttggag
      301 ggcttcggcg actacgccaa agtgtttctt gatttcttca acctgtgcat cagtcatggc
      361 acgcctgtat agcctgaatg accgggaaaa gcaacgcagc agggaactaa ctggcgaatg
      421 ggttattcac tgctctttcg tcgaaaccgc ttggtcagct cctcatatac cgatcgacgg
      481 tgacccaccg ccattaggcg aatgattcgt gttttttgat gaacggcata gatgatccga
      541 aagcggcgag cgcgatactt tcgtaacccg tcaagttccc ttgagattgg gatggagcga
      601 gtgcgtcacg tccgccacat gcggagggat gtccggccgg aaggaggtca cgccgtacgt
      661 cgggatgagt agttaaaaac taagggaaag agtaagattc agccttaacg acgtcccccg
      721 gatagacgag ccttaagctc ggccttacat ccggtagtca cgtgctcgcg ccaaggccgc
      781 tccactcaac aaccgttggc caaccttctt ccatttggtt ctctcgattg cttgatccag
      841 ggaccgctgc cacattttca tgctgaccgg gattacctgt tcgagggcgt gtggcaggtc
      901 ggtggaagaa cggaaccaga acggtcctga ctaagaattt tgaagagggg caaagcatgg
      961 cagtaccaag cgctttcagt tggatgtgtg ttttttggcc gggaaaattc aacctggagc
     1021 atgaacaata ccagaatgag gaagaccacg accatcccga cgccgaccaa ccatattgtg
     1081 acgcggtctc ttagtggaac aagaggtggt ggacgccttg caaagctcct caacgatgct
     1141 ttattgaaga taacgaagat cgcagagggg acaatgcgtg ccatgatcgt cgcccgagat
     1201 tggtcaaaga gcgtggagtg tagtcgtcag cccatatccc tcttattcag tgttaggtgg
     1261 cccaaccgac tcagacgatc cacctcgagg aacacaatcc tgccacggcc aatcctgcaa
     1321 ctcttgtgtg acctcttcca aggtacactg aggtttcgtt ctcactactt cgagaataag
     1381 attttcgaga ataagattac tgatagttac ttgttgcggc ctaaggccat ctgtcgctct
     1441 aacaacctcg gagccgcaga ttgatcataa gtcacagccc ttgcttacgg agcaccggac
     1501 taactaatag ataagcaagt tttagtccgc cttgaatcga cttaaagtat ttgcgagatt
     1561 gcttggaagg gtgttcaaaa cccgaggatt aggaaagcag tacaggttgg atttcaggct
     1621 gctggttgaa ttttttaagt ggtgttcgaa tttcgcacgg tgcgcaacgc gcgagggatt
     1681 gccgaatcag cctaggggct gg
//
LOCUS       DATMTI010000158         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_684816, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000158 DATMTI010000000
VERSION     DATMTI010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684816"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1500
                     /locus_tag="VN039_02660"
     CDS             <1..>1500
                     /locus_tag="VN039_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44920.1"
                     /translation="AFDEALYILMNGVGLGFSVESQHISQLPPVSERFEHTGSTIVVA
                     DSKAGWARAFRELIALLWQGQIPKIDISNVRPAGARLKTFGGRASGPQPLVDLFDFTI
                     GIARNAAGRKLTPIEAHRIMCKVGEVVVVGGVRRSALLGQSDLGDYELAHAKSGAWWE
                     STPELALANNSAAFYKKPSIGEFLQFWGDLYESKSGERGIINMEGLRNSGLAPRRDLS
                     QIQGLNPCAEILLRSKQFCNLTEVIVNPNDTLDDLENKVNIATIFGTMQSSLTDFKYL
                     RKAWKVNCEEERLLGVSLTGQMDHQILNGGNGTEMLATWLTEMCKKAVSTNERYAKQM
                     NINVSTAITTVKPSGTVSQLTDSASGMHVRHSKYYMRTVRADNKDPLTQFLKDVGIPG
                     EPDVMKPDATTVFSFPQKAPEYAKTREDLGALEQLEIWKTYKMFWTEHNPSITVSVRE
                     EEWIEVANWVYENWQYIGGISFLPYSEHTYKQAPYQAMTEAEYYLATAD"
BASE COUNT          386 a          342 c          405 g          367 t
ORIGIN      
        1 gcgcctttga tgaggcacta tatattttga tgaatggtgt tgggctcggt ttttcggtag
       61 agtctcaaca catatcacaa ctaccgccag tcagcgagcg tttcgagcat acaggcagca
      121 cgattgtcgt agccgactcg aaggctggtt gggctcgtgc tttccgcgaa ctcattgctc
      181 tgttgtggca gggccagatt cccaagattg acatttccaa tgtacgccca gcgggagcaa
      241 gattgaagac attcggggga cgtgccagtg ggcctcaacc tttggttgac ctattcgact
      301 tcaccatcgg tatcgcacgc aatgcggccg gtagaaaatt gacccccatt gaggcacatc
      361 gtatcatgtg taaggttggc gaggtagtcg ttgtgggagg cgtcaggcgt tccgcattgc
      421 ttggtcagtc agaccttggg gactatgaac tagcacacgc caagtcgggt gcatggtggg
      481 agtctacccc agaattagca ttggcgaata actcggcagc attctacaag aagccatcca
      541 ttggagagtt ccttcaattc tggggagacc tctatgagtc caagtccggt gagcgcggta
      601 tcatcaatat ggagggcttg agaaactcag gactagcacc acgtcgtgac ctatctcaga
      661 ttcaaggatt gaacccctgc gccgaaatcc tgttgcgctc gaagcaattt tgtaatttga
      721 cagaggttat tgtcaatcct aacgacactc tagacgactt ggagaacaag gtcaacatcg
      781 ctactatttt tggaacaatg cagtcgagtc tcacagattt taagtatctg aggaaggctt
      841 ggaaggtcaa ttgcgaggag gagagacttt tgggtgtttc tctaactggt cagatggacc
      901 accagattct caatggagga aatggtacag aaatgctggc cacgtggttg accgagatgt
      961 gcaagaaggc cgttagcacg aacgagcggt acgcaaagca gatgaacatc aatgtctcta
     1021 ctgccatcac aacagtcaag ccaagcggca ctgtttctca attgaccgat tctgcatctg
     1081 gcatgcatgt tcgtcactcc aagtactaca tgagaacagt ccgtgcagac aacaaggacc
     1141 cgctgacaca gtttttgaag gatgtcggta tccctggcga gccagatgtg atgaagccag
     1201 atgccacgac agtattctca ttccctcaga aggctcccga atatgcgaag actcgtgagg
     1261 atttgggagc attggagcag ttggagattt ggaagacgta taagatgttt tggacagaac
     1321 acaatccttc catcacagtc tcggtacgag aagaggagtg gattgaggtg gccaattggg
     1381 tctatgagaa ctggcagtat attggtggaa tatcattctt gccgtattct gagcatacat
     1441 acaagcaggc accctatcag gctatgactg aggctgagta ttatctggct accgccgact
//
LOCUS       DATMTI010000159         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_770009, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000159 DATMTI010000000
VERSION     DATMTI010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770009"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VN039_02665"
     CDS             <1..273
                     /locus_tag="VN039_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44921.1"
                     /translation="AAPYPRLSMETVLLEDPEVLIFPVGQAEGISESEQRTWRRWTGM
                     AAVKTGRLHQIPASILNRPGPRIVQALRMLAEIMHPELDLSLPVNR"
     gene            282..1430
                     /locus_tag="VN039_02670"
     CDS             282..1430
                     /locus_tag="VN039_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWV44922.1"
                     /translation="MATPTSQLAPADSSPSVSPPLTKSGTEQLRQWKSSGAILTFQRW
                     ATIIGGLAVASLIVCVLSLQFGATKIDMRTVMTVLSRWSGVGAPDTATSDTSAMIMFH
                     IRLPRVLTGFFVGGSLAVVGVTLQALLRNPLADPYVLGVSSGAALGVSLAMLSGIGTT
                     LAFLPGLPLFGFIGGLLSLVVMYRLARSHGRLPIQSVLLAGIILNAMLTALIMFITSI
                     MDPNRSAGLMAWLMGSLTVSNYGMLSMFALYVLGGASLLLQKAQIMNILTFGEETARS
                     LGVDTEQVKKRLFFLAALLTGAVVSVSGMIGFVGMIVPHAVRMLVGSDHRVLLPASMF
                     AGGMFLVAADTVARTILAPTEIPVGIVTALTGGPMFLYLLLWRKDQLV"
     gene            1427..>2039
                     /locus_tag="VN039_02675"
     CDS             1427..>2039
                     /locus_tag="VN039_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV44923.1"
                     /translation="MIDVDEHQLPSEASTDATAHGGPGIAYEIESMRFRYHAGSSDGA
                     GWILDDVGFQVTEGEVLGIVGPNGSGKTSLLKILAHVLIPQQGTLRLFGNDLATLTPQ
                     DMARLVAYVPQDTQQSFPFTVAETVVMGRFAQRKHTVWSGGFGWEDETDIAVAAQAMA
                     TVDVAHLAQRLVPDLSGGERQRTVIARALAQSPMVLLLDEPTAF"
BASE COUNT          382 a          557 c          613 g          487 t
ORIGIN      
        1 gccgcaccct acccacgtct gagcatggaa acggtattgt tggaagaccc tgaggtattg
       61 atctttccgg tgggccaggc cgaaggaatt tccgagagtg aacaacggac ttggcggcgg
      121 tggaccggga tggcagcggt caaaacgggg cggctgcacc agattcccgc cagtattttg
      181 aaccgcccag gaccgcgtat cgtgcaggca ctcaggatgt tagcggaaat tatgcatcct
      241 gaactggatc tgtcgttgcc ggtgaatcgg taaggggtca tatggccacg ccgacttcgc
      301 aactcgcacc ggccgattcg tccccgagcg tttctcctcc gctgacgaaa tccgggaccg
      361 agcagctgcg gcaatggaaa agcagcggag caatcctgac gtttcagcgg tgggcgacga
      421 tcatcggcgg cctcgccgtc gccagtctaa ttgtgtgtgt gttgtccctc cagtttgggg
      481 ccacaaagat cgatatgcga accgtcatga cggtgttgag ccgatggtcg ggggtcggag
      541 cgcccgatac cgcaacaagc gatacctccg cgatgatcat gttccatatt cgtctgcccc
      601 gtgtcttgac gggttttttc gtcggaggca gcctcgcggt cgtaggcgtg acgctgcaag
      661 ccctcctgag aaaccctctc gcggatccct acgttcttgg cgtctcaagc ggagcagcat
      721 tgggagtatc gttggccatg ttgtccggca tcggcacgac attggctttt ttgccgggac
      781 tgcctctctt cggatttatc ggcggcttat tatctctggt cgtgatgtat cgattggccc
      841 gttcgcatgg gcggttgccg attcaaagcg tgttgttggc cggcattatt ctgaacgcca
      901 tgctgacggc gttgatcatg ttcatcacat cgatcatgga cccaaatcgg tcggccggac
      961 tcatggcgtg gttgatgggc tccctgacgg tgtcgaacta tggcatgctt tcaatgtttg
     1021 ctttgtatgt gctcggagga gcgtctctac ttctgcaaaa ggctcaaatc atgaatatcc
     1081 tgaccttcgg cgaggagacg gcgcgctcgc tgggtgtcga caccgaacag gtaaaaaagc
     1141 gcttgttttt cctcgctgca ttgttgacag gagcggtcgt atcggtgagc ggcatgatcg
     1201 gctttgtcgg catgatcgtg ccgcatgcgg tccgtatgct ggtcgggtcc gatcatcgcg
     1261 tgctgttacc ggcttcgatg ttcgcaggtg gaatgtttct cgtagcggct gacactgtcg
     1321 ctcgaaccat tctggcaccg accgaaattc cggtggggat cgtcaccgct ctcaccggcg
     1381 gtccgatgtt tctctatctg ctcctgtggc gaaaggatca attggtgtga tcgacgtgga
     1441 tgagcaccaa ctcccatctg aggcatcgac cgatgcgacc gcccatggcg ggcccgggat
     1501 agcctatgaa atcgaatcca tgcgatttcg ctatcatgcg ggatcatcgg atggtgccgg
     1561 ctggattctc gatgacgtgg gttttcaggt cacggaggga gaagttctcg gcatcgttgg
     1621 gccaaacggg tcaggaaaga cctcgttgct caaaatactg gcacacgttc tcatcccgca
     1681 gcaaggaacg ctccgcttgt ttggaaatga ccttgcgacg ttgactccac aagatatggc
     1741 tcgtctcgtc gcttatgtcc ctcaggatac tcaacagagc tttcccttta ccgttgcgga
     1801 gacggtcgtg atgggacgat tcgctcaacg gaaacatacc gtatggtccg gagggtttgg
     1861 atgggaagat gagacggata ttgccgttgc cgcgcaggcc atggctacag tcgacgtcgc
     1921 ccatcttgcg cagcgcctgg tgccggatct ttccggcggc gagcgacaac ggaccgtgat
     1981 cgcccgcgcc ttggctcaat ctcccatggt cctgttgctc gacgaaccca cggcctttc
//
LOCUS       DATMTI010000160         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_684893, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000160 DATMTI010000000
VERSION     DATMTI010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684893"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VN039_02680"
     CDS             <1..595
                     /locus_tag="VN039_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV44924.1"
                     /translation="FGAASDQSEAYFFAKTGESALLPEEAQESGTHNIERESLQQRRQ
                     VLESCLADLELRRRLQATRKKKGIDTALVEDYMSHDVRSIHKEATIKQAGQLLQKHKV
                     GSLIVDDGSRYIGILTDSDLTRKAVAKGLDPNTTTVATCMSRPLVTIEEDESLAEAMS
                     LMKKQSIRHLPVTADATIIGVLSVSDLLRAFEEQKTP"
     gene            733..>1580
                     /locus_tag="VN039_02685"
     CDS             733..>1580
                     /locus_tag="VN039_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV44925.1"
                     /translation="MKPLFTDLSLGLCEGDHVGLIGPNGSGKSTLLKILAGLEDPDRG
                     TRSMRRQLRIGYVPQEPSFADQHSVEDALSQVLLDEGLDPHEQGARLAKAISLGGFVR
                     SDQSVSTLSGGWKKRLAIARSLILEPDVLLMDEPTNHLDVEGILWLESLLKAEPHAFI
                     VISHDRRFLESVTTRVWELNRRYVNDVFQVNGRYSEFLEQRDAALQAQADYQASLANR
                     VRREVEWLRRGPKARTTKAKARIDSAGRLIDELNDIESRQGNAPAGIDFTASGRKSKQ
                     LLVAKG"
BASE COUNT          378 a          419 c          462 g          321 t
ORIGIN      
        1 gttcggcgcg gcatcggacc aatcagaggc ttacttcttt gcaaagacgg gagaatcggc
       61 gcttttgcct gaagaggcgc aggaaagtgg aactcacaat attgaacggg agagcctgca
      121 acagcgacgg caagtattgg aaagctgtct ggccgatctc gaattacgtc gacgactgca
      181 ggcaacgagg aaaaagaagg ggatcgacac ggcacttgtt gaagactata tgtcacatga
      241 cgtgcggagc atccacaaag aagcgacgat caaacaagcg gggcagctat tgcaaaagca
      301 taaggtcggc tcgttgatcg tcgacgacgg gtctcggtat atcggcatcc tcaccgattc
      361 agacctgaca cgcaaagccg tcgccaaagg gcttgacccg aataccacga ccgtcgccac
      421 ttgcatgagc cggccacttg ttacgattga ggaagatgag tccctcgccg aagccatgtc
      481 gctgatgaag aagcagagca tccgacacct gccggttacc gccgatgcca cgatcatcgg
      541 ggtactttca gtctctgatc tccttcgcgc gtttgaagaa caaaagactc cgtagtcctc
      601 gtacagctca ctgctagtag gtgcgcgaaa cggccgccga aaagtctgct cacgagagct
      661 ccgatcacgt acactgctgt catgccgcca atcctactgc tcagttgcga gtccctcggg
      721 aagagctacg gtgtgaagcc gttgtttaca gacctgtcgc tcggactctg tgaaggcgat
      781 cacgtcgggc tcattggtcc caacggttct ggaaaatcga cgctgctcaa gattctagcc
      841 ggtctggagg atccggatcg cggcaccaga tcgatgagaa ggcagcttcg tatcggctat
      901 gtgccgcaag agccctcatt tgccgatcaa cactcggtcg aagatgcact gtcccaagtc
      961 cttctggacg aggggttaga cccgcatgag cagggagcgc gtcttgccaa agccatcagc
     1021 ctcggcgggt ttgttcgttc tgatcaatcc gtctcgacgt tgtcgggggg atggaagaaa
     1081 cggctggcga tcgcgcggtc gttgatattg gaaccggacg tgctgcttat ggatgagccg
     1141 accaaccatt tggatgtcga aggcatcctg tggcttgaaa gtctgttgaa agccgaacct
     1201 catgccttca tcgtcatcag tcatgatcgg cgattcttgg agtcggtgac cacgcgggtc
     1261 tgggaattga atcgccgata cgtcaatgac gtctttcaag taaacggccg gtacagcgag
     1321 tttttggaac agcgcgacgc ggcattacag gcgcaggccg actatcaagc gtcgttagcc
     1381 aatcgggtgc gtcgggaagt ggagtggctg agacgcgggc cgaaagcccg cacgactaaa
     1441 gctaaggccc gtatcgattc ggccggtcgg ctgattgacg aactaaacga tattgaatcg
     1501 cgccaaggaa atgctcccgc cgggatcgac ttcacagcct caggccgaaa gtccaagcag
     1561 ttactggtcg caaaagggat
//
LOCUS       DATMTI010000161         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_219070, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000161 DATMTI010000000
VERSION     DATMTI010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219070"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1395
                     /gene="hpnH"
                     /locus_tag="VN039_02690"
     CDS             379..1395
                     /gene="hpnH"
                     /locus_tag="VN039_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HWV44926.1"
                     /translation="MAVPISQMYTVATYVLSQKLKGVKRYPLVLMLEPLFRCNLACAG
                     CGKIQYPDHILDKRLTPEQCWAAAEECGAPMVSIPGGEPLIHPEIAKIVQGLVDRRKY
                     IYLCTNAILLERKLDEYQPSKYLTFSVHMDGLKDEHDLAVCRDGVYEVAVKAIKAALK
                     RGHRVTTNTTLFDDANPERVRTFFDEMMALGVEGMTISPGYSYQKAPDQQHFLKRART
                     QELFSKILARPKAGWQFNQSPLFLDFLMGRREYQCTPWGNPTYNVFGWQKPCYLLQEG
                     YTTTFRELMESTEWEKYGTGRNEKCADCMVHCGYEASAVEDTFSTASGFARTAKLTLM
                     PTSR"
     gene            1478..1882
                     /locus_tag="VN039_02695"
     CDS             1478..1882
                     /locus_tag="VN039_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44927.1"
                     /translation="MTDTKSHAVDHPGSLADSLEGRVFTPTSLTETAEAVELAFDYRG
                     DVTLLLRSGESIAGYLFNRQAAGGNSYLELFPVGHQNPQRIPYQSIESIAFTGEDTAN
                     GKSWEAWVSKKDSERRAEAEQIEADARTKGYL"
     gene            2027..>2228
                     /locus_tag="VN039_02700"
     CDS             2027..>2228
                     /locus_tag="VN039_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44928.1"
                     /translation="MRALGGSFDLQSAPGQGTTATLTLQMAAGAESKGLGAELSGSNA
                     ALKTQSSALQQNATVRILLVDDH"
BASE COUNT          526 a          593 c          620 g          489 t
ORIGIN      
        1 atatggaagt attttcgatc atatcgaatg aatgccctca tcagggcctc cttgagccaa
       61 gaacttgtcg caccctaatc gttgtccacc gacgtgtgaa cagttggaac gccagaaagg
      121 acggcgagag aggagagaga cacgtctcat agctacctca gctagggacg ctgagatcgg
      181 cctggcgcaa gtcaatccga ccttagcaga aaccctgata ctgcggcaag tggaggcatc
      241 ccctctttgt tcttcgaaat tgacgagccc tatgcatgat gttagaattc caaaaatttt
      301 gtgcctccaa gcagggctgc ttcggcagtc gttcagatag aatagattgc aaaaaaccga
      361 ttaagtaagg agaacggaat ggctgttccg atttcccaga tgtataccgt ggcgacgtat
      421 gtgctctccc agaagctgaa gggcgtgaag cgctaccctt tggtgctgat gctcgagccg
      481 ctctttcggt gcaacctggc ctgtgcgggt tgtggaaaga tccagtatcc cgatcatatc
      541 ctcgacaagc ggctgacgcc ggaacaatgc tgggcggcgg cggaggaatg tggagcgccc
      601 atggtgagca ttccgggagg ggagccgctc atccatcctg aaatcgcgaa gatcgtccag
      661 ggcctggtgg atcggaggaa gtatatctat ctctgtacga acgccatcct cttggagcgg
      721 aagctggacg agtaccagcc ctccaaatac ctgaccttca gcgtccatat ggacggactt
      781 aaggacgagc acgatttggc cgtttgccgc gacggagtct atgaggtggc ggtcaaggcc
      841 atcaaggcag cgctcaagcg cggtcatcgg gtgacgacca acaccacgtt attcgatgat
      901 gcgaatcctg agcgagtcag aacatttttc gatgaaatga tggcgttggg tgtcgaaggc
      961 atgacgattt caccaggcta tagttatcaa aaggcaccgg atcagcaaca tttcttgaag
     1021 cgggcgcgga cgcaagagct cttctccaag attcttgccc gtcccaaggc tggttggcag
     1081 ttcaatcaat ccccgctgtt tttggatttc ctgatgggtc ggcgtgagta tcaatgtacg
     1141 ccctggggca atccgaccta taacgtcttc ggatggcaga agccctgcta tttgctgcaa
     1201 gaagggtata caacaacttt ccgagagctg atggaatcga cggagtggga gaagtacggg
     1261 actggtcgaa acgaaaaatg cgccgattgc atggtccatt gcggctatga ggcatcggcg
     1321 gtggaagata cgttcagtac ggcctccgga tttgctcgga cagcaaaatt gacactgatg
     1381 cctacttctc gttaatcgtt aaccgttcaa cgtgaatcgc agtcttccaa tgtttctcgt
     1441 ttaacgaata acgagtcacg gttaatggat tccgctcatg actgatacga aaagccacgc
     1501 ggttgatcat cctggttctc ttgccgattc ccttgaaggt cgagtgttca caccgacttc
     1561 cttgaccgaa acagccgagg ccgtcgagct ggcgtttgac tatcgcggcg acgtgaccct
     1621 actgctacgg tccggcgaat cgatcgctgg atatctcttc aatcgtcaag cagccggcgg
     1681 caattcctac cttgaactgt ttcccgttgg tcatcagaat cctcaacgca ttccctacca
     1741 gtccatcgag agcatcgcgt tcaccggcga agataccgcg aacggcaagt cgtgggaggc
     1801 ttgggtctcg aagaaagact ccgagcgtcg agcagaagcc gaacaaattg aggcggatgc
     1861 gcgcacgaag ggctacctct aatgcccagc tctccccgct gccgccgatg cgcaacggtg
     1921 tctcgcttgt gctcgtacgg ccgctgcccc cgcaaagcgt gagcaagggg ttcacaatac
     1981 ctcgccgttc tcctcgaaat tcgggctctt cagcatccgc gaacggatga gggcgctcgg
     2041 tggaagtttt gacctccaat cagcaccagg acagggcacc acggccacgc tcacgctgca
     2101 gatggcagcc ggtgctgagt cgaaagggct gggtgctgag ttatccggta gcaacgcagc
     2161 actcaaaact cagtcctcag cacttcagca gaacgcgacg gttcgcattt tactagtaga
     2221 cgatcaca
//
LOCUS       DATMTI010000162         1957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_770153, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000162 DATMTI010000000
VERSION     DATMTI010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770153"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1256)
                     /locus_tag="VN039_02705"
     CDS             complement(84..1256)
                     /locus_tag="VN039_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44929.1"
                     /translation="MRYISIIGGVVVALAFAATATAGSFEDKAQVTVSGAEYMLNGYI
                     VKVEGNAYWIRKASGEVVRVAITEDTHLICPTRTGNKAAKVESKPGAGFRMGDCPFIR
                     GDTVKVEVTDLGSASLIRFDVSPASETVNLGLPQGWEGYMPNDGVLSFKAGPRYPVYT
                     KDGQAVGHLAGTLTDTLLGTEYALIELEGDGQMMTVPRTAMRQEWSEPNKPRVALLIN
                     KHQLGEMAPPTYQAANLISVSEVRDFWAKAAPPEPFVPSEVDVVIDISEKNFQITKGG
                     TARGFALMAGMPAVIKLLNRDLVAHEFMSTLFRDVPFRISGNATAVKTAQASGFRIDP
                     GQTVTIEFNAPVAKPDRYGDRASTYDVFWCNIHGKEHGQQMRGELAIIETTGIGGG"
     gene            complement(1610..>1957)
                     /locus_tag="VN039_02710"
     CDS             complement(1610..>1957)
                     /locus_tag="VN039_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44930.1"
                     /translation="IPQYVHHLYHYTHSFVIFLAVFFLVWLFLKRPVWELGAWGFHVL
                     ADVPLHSYAFFPTPVLWPLSDWKFDGWQWMTPGILITNFALLSLLYVWYIRQLFLARK
                     KEEARPETISRAT"
BASE COUNT          452 a          529 c          528 g          448 t
ORIGIN      
        1 atgtactccg tggggaagaa tttctgtgta ggtgtgcact ggcaactgat tggatcttca
       61 ccgtgccctg gtgcttcatc gcttcagccg ccgccgattc ctgtggtctc tataatcgcc
      121 aattccccac gcatttgttg cccatgctcc ttcccatgga tattgcacca gaagacatca
      181 taggtgcttg ctctgtcgcc atatcgatcc ggcttggcca cgggggcgtt aaactcaatg
      241 gtgacggttt gccctggatc gatgcgaaag ccggaggctt gtgcagtctt gactgcggtg
      301 gcattaccag aaatgcggaa cgggacatcc cggaacagtg tagacatgaa ctcatgcgcg
      361 acgagatccc ggttgagaag tttaatgacc gccggcatcc ctgccatgag agcaaatcca
      421 cgcgcagtcc ccccttttgt gatttgaaaa tttttttcgc tgatatcaat gaccacgtca
      481 acttcgcttg ggacgaaagg ttcgggaggt gcggcctttg cccagaaatc acggacctcc
      541 gagaccgaaa taaggtttgc agcctggtac gtaggagggg ccatctcccc aagctgatgc
      601 ttgttaatca gcaacgccac acgaggtttg ttgggctccg accattcttg tctcatcgcg
      661 gttctgggca cggtcatcat ctgcccgtcc ccttctagct cgattagtgc atactcggtt
      721 cctaatagtg tgtcggtcag tgttccagcg agatggccga cagcctggcc atccttggtg
      781 tatacgggat agcgaggccc cgctttgaag gaaagcaccc catcattggg catgtagcct
      841 tcccatccct gaggcagtcc aaggttcaca gtttcagaag ctggagacac atcaaaccgg
      901 atgaggctcg cgctgccgag gtccgtgacc tcgaccttca cagtgtcccc tcggataaat
      961 ggacagtccc ccattcgaaa accggcgcca ggcttcgatt ccactttcgc cgccttgttg
     1021 ccggtgcgag tcgggcagat caggtgagtg tcttccgtga ttgcgacgcg aacgacctcg
     1081 cccgacgcct tcctgatcca gtaagcattc ccttcgacct tgacgatata gccgttcaac
     1141 atgtattcgg caccactaac cgtcacttgc gccttatcct caaacgaccc ggcagtcgct
     1201 gtcgcagcga aggcgagcgc gacaacgact cctcctatga tgctgatgta acgcatggag
     1261 caatcctcct tgctataggt tatcttgctg aatctgctgg caccatgttc tccgtctgct
     1321 gtcgcacgtc tagactcatc aaggcgtgag acatcgttga ttgatgggaa tgaaatgccg
     1381 aggttgaatc aaataaattg gcgatcagat cactgaggta tcgggccgag gcggagagga
     1441 aaactagaga acgcaagagg aggggaggtg acatcgcatc ccccttgcga aagggtcgat
     1501 tacgtacagc ctactcttct gttgacgatg atgcaagcga cgcatcaggc atctcagaaa
     1561 ttagggatat accgtccagg aaatgttgcg agatcctgtg agttgtcatt catgtggccc
     1621 gcgaaatggt ttctggccga gcctcctctt tcttcctagc caggaacaac tgccggatgt
     1681 accagacata gagcagcgac agcagcgcga agttggtgat gagaataccc ggcgtcatcc
     1741 attgccagcc gtcgaatttc cagtcagaga ggggccacag taccggcgtc ggaaaaaagg
     1801 cgtacgaatg caacggcacg tcggccagga catggaatcc ccacgctccc agttcccaga
     1861 ccggtcgttt taggaaaagc catacgagga aaaagacggc gagaaatatg acgaagctat
     1921 gagtgtaatg atagaggtga tgcacatatt gaggaat
//
LOCUS       DATMTI010000163         3824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_6529, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000163 DATMTI010000000
VERSION     DATMTI010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6529"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="VN039_02715"
     CDS             <1..1690
                     /locus_tag="VN039_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV44931.1"
                     /translation="YWRLNETVQIDLSRERERAEAASIAKSQFLATMSHEIRTPMNGI
                     IGMTGLLLDTALSAEQREYAETVRRSSEHLLDIINEILDFSKIEAGRLDLESLNFDVH
                     TTVEDTVGLLGERAYAKGLELTCLVQAGVPTALRGDPGRLRQILVNLIGNAIKFTDQG
                     EVVVTVSMEDKPGEMTVASSDSSYPTLRFEVSDTGVGITQEQQAKLFQPFTQADGSTT
                     RKYGGTGLGLAICRQLVEMMGGRIGVNSTAGQGSAFWFTSRLNLRTEGTQTTFPLQTA
                     LHDSRILIVDDHATNRRVLEQSLRGQGVMYESAENGYQALECLHNAASRQTHFDVAIL
                     DMQMPGMDGLELARRIKSEAAISATRLVLLTSVGQRGDAKAAQSAGIAAYLTKPIRQS
                     LLYECLSLVLANSSGAAPSVTQPTASIITRHSLAEVQARSRGRILVAEDNPVNQKVAV
                     KMIEKLGYRVDIAGNGCEAVEASERIPYDLILMDCHMPEMDGFEATRKIRARKLGVGY
                     IPIIAMTANAMQEDRDRCLDVGMDDFLSKPVMSKSLAAVLNRWLPHDTASSQAA"
     gene            1719..1964
                     /locus_tag="VN039_02720"
     CDS             1719..1964
                     /locus_tag="VN039_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44932.1"
                     /translation="MGLSILLLSPPFLIISNSLTDLEAQFKSESEALAAATDPLNEKL
                     ETISIKPAKSNIAVKLVTLAWTPPWRDATGGLTPACT"
     gene            2409..3038
                     /locus_tag="VN039_02725"
     CDS             2409..3038
                     /locus_tag="VN039_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV44933.1"
                     /translation="MFVLIVDDHPLLRQAIRGVIESRFPSSVVQEASTGEEAVRIVRQ
                     ERVDVAILDIGLPDFSGLTVLRRVKQVRPSLMCLVLTMHDNPQYVRLAMAHGASGYLT
                     KGATSGELYDAIRTILSGRQVVMELFRETLDRRSMRRDVIWPHESLSVRELEVLSLVA
                     KGRTVSQVAKRLKLSVKTVSTYRTRLLEKLHLGTTADLIRYAVDHQLVR"
     gene            3174..>3824
                     /locus_tag="VN039_02730"
     CDS             3174..>3824
                     /locus_tag="VN039_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV44934.1"
                     /translation="MRQKDHGHGRKQQNQKWIVLDASRITTSRSRPIRVFIADDHEVV
                     RAGVCALLEGERDVEVVGEADNAEGVLTESQRTKPDVLLLKSGLSGGSDSDIYKTLFN
                     VLPAVQIISLMRDGDAEAFRNAVEGGAQGYLWEKTGRIELIRAIRTVAKGNSYLGPET
                     AAQTFRLLRARQDSVCSPSALHTLSRQERRVIALIAEGNTNKQIATKLVLSEKTVKN"
BASE COUNT          891 a         1028 c         1093 g          812 t
ORIGIN      
        1 ctattggcgt ctcaatgaaa cagtgcagat cgatctctcc agagaaaggg aaagagctga
       61 ggctgccagt atcgcgaaat ctcaatttct agccacgatg agccatgaga ttcgaacgcc
      121 gatgaacggc atcatcggga tgaccggact gctcctcgac actgcactct ccgccgaaca
      181 gcgagagtat gcggagacgg tccgtcgctc cagtgaacat ttgctggaca tcatcaacga
      241 gattctcgac ttttctaaaa tcgaagcggg caggctcgac ctggagagcc tcaacttcga
      301 cgtgcacacg acggtggaag acaccgtcgg cttactcggc gagcgggcct atgccaaagg
      361 ccttgagctg acctgcctcg tgcaggcggg tgtgccgacg gcgctgcgag gggaccccgg
      421 ccgtttgcgg caaatcttag tgaatttgat cggtaatgcc atcaaattca cggaccaggg
      481 tgaggtcgtg gtcacagtga gcatggagga taagcccggc gaaatgacag ttgcaagctc
      541 ggattcgtcg tatccgacgc tacgatttga agtatcggac accggagtcg gcatcacgca
      601 ggagcaacaa gcgaaactgt ttcagccatt cacgcaagcc gacggctcca ccacaaggaa
      661 atatggaggc accggcctgg gccttgccat ctgcaggcag ctcgtggaga tgatgggagg
      721 aaggatcggt gtgaacagca cagccggaca gggcagtgcc ttttggttca cctctcgact
      781 caatctcaga acggagggaa cccagaccac ttttccgctt cagacagcac tccatgacag
      841 tcgaattctg attgtggacg accatgccac gaaccggcga gtgctggaac agagtctacg
      901 aggacaaggg gtgatgtatg aaagcgcgga gaacgggtat caagcactgg aatgcctgca
      961 caacgcggcc agccggcaga cacatttcga tgtagctatt ttggatatgc aaatgccggg
     1021 catggacggt ctggaattgg cccgccggat caagtcggaa gcagcgatca gcgcgacccg
     1081 tctggtactg ctgacatcgg tgggtcagcg cggcgatgcc aaagcggctc aaagcgccgg
     1141 tatcgccgcc tatctgacga aaccgatccg gcagtcgctc ttatacgagt gtctcagcct
     1201 cgtccttgcc aattcctccg gcgctgcgcc ctctgtcact cagcccacgg cgtcaattat
     1261 cacccggcat agcttggcgg aagtccaggc tcggtcccgg gggcgtattc ttgtggcgga
     1321 agacaacccg gtcaatcaaa aggttgcggt aaagatgatc gaaaaactcg gttatcgagt
     1381 ggacatcgcc gggaatggat gtgaggcggt cgaggcgagc gagcggatcc cttacgactt
     1441 gattttaatg gattgtcaca tgcctgagat ggacggtttt gaagcgacca ggaagattcg
     1501 cgcgcgaaaa ctcggagttg ggtatattcc gatcatcgcc atgacggcca atgcgatgca
     1561 ggaggatcgt gaccggtgcc tggacgtcgg gatggatgac ttcctcagca agccggtcat
     1621 gagcaaatcc ctggcggcgg tcctgaatcg ctggttgccg catgacacag cctcttcgca
     1681 ggcggcatag gggacatata cccacaaccc gttacgccgt gggactgtct atcctcctac
     1741 tgagtccgcc gttcctcatc atttccaact ccctgaccga tctcgaagcg caatttaagt
     1801 cagaaagcga agcattggcc gccgcgaccg accctctgaa cgaaaaactc gaaacgattt
     1861 caatcaagcc cgctaaatcc aacattgccg tcaaacttgt gaccctcgcc tggacaccgc
     1921 cttggcggga tgcaacgggc ggcttgactc ctgcctgtac gtaactcaag cctttcttat
     1981 cggtacgatg gagcgtgagc cgttctattc gagacggatt ttataaggta tactaatgtt
     2041 atagtacgag gaagggtccg ctgccgggtt cggtagcatc agccctcgct ggccgaccat
     2101 atggcgaatc gtctcaatct cggtcggccg gcaatgctgc atgaaagact ctcggatcgg
     2161 gagagggaag tgtatgccat cgaatatcgc ttagtgacct gaaaaattta tgtcaaccat
     2221 ccttccatgg tttgtgagca gcacatcgca agtaaggcaa gtgtcttccc ttccgtaccg
     2281 gatatcacgg ccgattgttc catcattgct ccatttgtag gacggctgcc gacatccgga
     2341 tcagttcttg tctgctatct ctatctcgag cccggcgtta tggattcaaa cgaaaggcac
     2401 gagcacccat gtttgtcctg atcgtcgatg accatcctct tcttcgccaa gctatcaggg
     2461 gagtcatcga aagccgtttc ccttcatcgg tggtacagga agcgtctacc ggagaggaag
     2521 ccgtccgtat cgtacggcaa gaacgcgtgg atgtggcgat tctggacatc ggtctcccgg
     2581 atttcagcgg cttaaccgtg ttgagacgtg tcaaacaagt gcgtccttcc ctcatgtgcc
     2641 tggtgctgac catgcatgac aatccccaat atgtgcgtct tgccatggct catggggcct
     2701 ccggctatct caccaaagga gcgacgtcag gggaactgta cgatgccatc cggaccattc
     2761 tgtccggacg tcaggtagtg atggaattgt tccgcgaaac actcgaccgc cgttcaatgc
     2821 gtcgtgatgt gatatggccg catgaatccc tttccgtgcg cgagttggaa gtcctctccc
     2881 tagtagcgaa aggccgcacg gtctcacagg tcgcgaagcg attgaagctg agtgtcaaga
     2941 ctgtgagcac gtatcgaacc cggttgcttg aaaagctgca tcttggaacg actgccgatc
     3001 ttatccgtta tgccgtcgat catcagcttg tgcgctaaga accgatgatt cccctgtagg
     3061 acaaaaaccg ataccggggt gaggttcgac ctgataacta ggctcggcgt tcctgggagc
     3121 ttctgagaag aatcgagtca gtgcggcgac tcctactcac cggggttgtt tggatgcgac
     3181 agaaggatca tggccatgga cgtaagcaac aaaaccaaaa gtggattgtc ctcgacgcct
     3241 caagaatcac gacttcacgc tctcgaccga tccgcgtatt tatcgccgat gatcacgaag
     3301 tcgtcagagc cggggtgtgt gcattgttgg agggagagcg agacgtcgag gtggtcggtg
     3361 aggccgacaa tgcggagggc gtactcaccg aatcgcaacg aaccaagccg gatgtgcttt
     3421 tactgaagtc cgggctttca ggcggttcgg actccgacat ctacaaaacc ctctttaacg
     3481 tccttccagc agttcagatc atcagtttga tgagagatgg tgacgctgag gcgttccgca
     3541 acgcagtcga ggggggcgca caagggtatc tatgggagaa gaccggccgg atagagctga
     3601 ttcgagcgat tcgtaccgtc gcgaaaggaa attcatacct cggcccggag accgccgctc
     3661 agacctttcg cttgctgaga gccaggcagg attcggtttg ctctccttcc gcgttgcata
     3721 cgctatcacg gcaggaacgg agagtgattg cgctcatcgc cgaagggaac accaacaaac
     3781 agatcgccac gaagctggtg ctgtcggaaa agacggtaaa aaat
//
LOCUS       DATMTI010000164         4642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_48708, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000164 DATMTI010000000
VERSION     DATMTI010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48708"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VN039_02735"
     CDS             complement(<1..230)
                     /locus_tag="VN039_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV44935.1"
                     /translation="MKVVTLADFQQFTGDKMKKNNLFQTERFFCDIYCFEPGQEQKGH
                     VHGDQDKAYIVLAGQGTFQVGADHRMLEAGQG"
     gene            complement(271..708)
                     /locus_tag="VN039_02740"
     CDS             complement(271..708)
                     /locus_tag="VN039_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44936.1"
                     /translation="MNRGWLAAGAVSAVVANLVPWSGLAAESTASAEKVVKETQEAID
                     ATKEYTAQQKEAFQRKAHQELTAIHRQIIRLREKITKASESTRADLQKSLNELEKKKD
                     GVKERLDELKGATDAKWHEVREGMNDALNELKHSYQKLLSHMP"
     gene            839..1702
                     /locus_tag="VN039_02745"
     CDS             839..1702
                     /locus_tag="VN039_02745"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWV44937.1"
                     /translation="MASEIGSVATPVERLEQALIQKIEAGEVELPLLPQAASQVLALA
                     ADPASDAAKLSSLIHQDQALAAHVMRIANSPAYMPRSPVVSLQHAVAMLGITLLSEIA
                     FTASLKSGAFKAPGHEDDVKRLWRHSLASGAFAKEVARMRRVNVESAYLCGLLHEIGK
                     PVVLQTVTTLAHVQQIALEKSVLYGWVDGYHSRVGALIAEKWSLPKQVAAAIQYYEGY
                     DHADSFRQECLLTCAADRLASHLLTPDDVPEETLRAHPVFGELNLYPNDIDQLLTRKD
                     HVLAVVNALNL"
     gene            1699..3114
                     /gene="sthA"
                     /locus_tag="VN039_02750"
     CDS             1699..3114
                     /gene="sthA"
                     /locus_tag="VN039_02750"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HWV44938.1"
                     /translation="MSTSVAFDIVVIGAGPAGQKAAVQGAKAGKRVALIERERGIGGS
                     CVYRGTIPSKTLRESALHLDRLKRAGEAFQFSLKPDTQIATLLSRLEDVVQAHDSFMS
                     RQIRRNGISLFHGRARFLSEHTIEMQTVDGVQQQFTADTIVVATGSRPRNPEAIPVDH
                     EHILDSDSLLSMMYLPRSLAVVGGGVIGCEYASIFALLGVEVTLIDRASYPLQFMDRE
                     LVEQFIKGFEQQGGHYLGGQHIEKVRWNEVAHVVTKLSDGTTINTEKMLVALGRQANV
                     EDLNLSAAGIMVSDKGVIPVSQYCQTNVPHIYAVGDMVGAPALASKAMEHGRRAVRHA
                     LNLPIGDAASTIPLGIYTIPEMASIGLDEMAARERYRDPLIGRAKYEEVARAQISGAS
                     HGLLKMVADPAGERLLGVQVVGDSATELVHLGQLALQSGATIESFIDNVFNFPTYAEA
                     YRIAALDILGQAAKIRTAKAA"
     gene            complement(3152..>4642)
                     /locus_tag="VN039_02755"
     CDS             complement(3152..>4642)
                     /locus_tag="VN039_02755"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWV44939.1"
                     /translation="RGRTMGREGGVGRMKLNTEMRVNRISDEVWEIPVTEKSGMLVPA
                     RVYATPAILQSMDAGVFEQVTNVACLPGIRRYALCMPDGHWGYGFPIGGVAAFDVRSG
                     VISPGGVGYDINCGMRLIRTDLIRKDVQPHLDRLMTELFRRVPAGVGAGGFVRLNRSS
                     FEQVMTNGARWCIEQGFGWHRDLNAIEEGGCITGADPSRISDQAVSRGINQLGTLGSG
                     NHYLEVQVVSSDRVFDKETATAWGIAGQDQIVVMVHCGSRGFGHQVASDYLKVFERAM
                     RRYGISVKDQQLACAPFTSVEGQDYFSAMNCAANTAFANRQVITHQIREAFAAVFSRP
                     AEELGMELVYDVAHNIAKVERYPEGELVVHRKGSTRALGPGSLELSPRYRMTGQPVIC
                     GGSMETGSYLLVGTEGAVRDTFASTMHGSGRTMSRSQAKKTVRGEQIQKQMKQRGILV
                     KAVSMSGLAEEAGFAYKNISEVVEAVDRAGITKTVAELRPIGNIKG"
BASE COUNT         1047 a         1368 c         1165 g         1062 t
ORIGIN      
        1 atcccttgcc ctgcttcaag cattcgatgg tcggcaccga cctgaaatgt tccttgtccc
       61 gctagaacga tatacgcctt atcctggtcg ccatgaacat ggcctttttg ttcttggccc
      121 ggttcaaaac aatagatgtc acagaagaat cgctccgttt gaaacaggtt gttcttcttc
      181 atcttgtcgc cggtgaactg ttggaaatcg gcaagtgtca cgaccttcat tataaatact
      241 ccccctgtct cggcacatag gaaccgacag tcacggcata tgggacaaca gtttctggta
      301 tgaatgtttg agttcgttca gggcatcgtt catcccttcc cgaacttcgt gccactttgc
      361 atcagtggcc cctttcagtt catccaatct ctctttgaca ccatcctttt tcttttccaa
      421 ctcgttgagc gacttctgaa gatcggctcg tgtggactca gaggctttcg taatcttctc
      481 acgcagccta atgatttgcc gatgaatcgc cgtcaattcc tggtgtgcct ttcgttggaa
      541 tgcctccttt tgttgagccg tatactcctt ggtggcatcg atcgcttcct gggtctcctt
      601 cacaactttt tcggcactcg ccgtcgattc agctgccaga cctgaccaag ggaccaggtt
      661 cgcaactacc gctgatacgg cacctgccgc caaccaacct cgattcatga ttccctcata
      721 atggaggcca agtatagtct gcgacgattc caatgtcacg accactaaac ttttacggga
      781 aagcagccga aaaagagagg tcgtaaccca cagactcgca ttcgaggagc agatccatat
      841 ggcttctgaa ataggatcgg tagccacccc agttgaacgg ctggaacaag ccttgattca
      901 gaaaattgag gcgggagaag ttgagcttcc cctgctccca caagcagcca gccaagtgct
      961 ggcgctggcg gcggatccag cctccgatgc ggcgaagctc tcatctttga ttcaccagga
     1021 tcaagcgctt gccgcgcatg tcatgcggat agccaattca cccgcctaca tgcctcgcag
     1081 tcctgtcgtc tctctccagc acgccgtggc catgctgggc attaccctgt tgtctgagat
     1141 tgcgtttacg gcttcattga aatccggcgc gttcaaagcg ccgggacatg aggacgatgt
     1201 gaaacgactg tggcgtcact ccttggcgag cggagccttc gcgaaggaag tcgcccgcat
     1261 gagacgcgtc aacgtcgaaa gcgcatacct ttgcggtttg ttgcacgaaa tcggaaaacc
     1321 cgttgtattg caaacggtga cgaccctcgc acatgtgcaa caaattgccc tggagaagtc
     1381 ggtattatat gggtgggtcg acggctacca ttcgcgcgtc ggtgccctca tcgcagaaaa
     1441 atggagtttg cctaagcagg tagcggcagc catccaatat tacgaaggtt acgaccatgc
     1501 cgattcgttc cgtcaagagt gcctgctcac ctgcgcggcc gatagactgg cgagtcacct
     1561 cctgactccg gatgacgtgc cggaagagac gctccgcgca catccggtct tcggtgaact
     1621 caatctatac cccaatgaca tcgatcagct actgaccagg aaggaccacg tgctggctgt
     1681 ggtgaatgcg ctaaatctat gagcacatct gttgcgttcg atatcgtcgt cattggagcc
     1741 ggcccagccg gccagaaggc agcggtccaa ggtgccaaag ccgggaagcg tgtcgccctg
     1801 attgagcgag agcgcggtat cgggggcagc tgtgtctatc gcggaacgat ccccagcaag
     1861 acattgcgag aaagcgccct ccatctggat cggctcaaac gagcaggaga agccttccaa
     1921 ttcagtctta agccggatac ccaaattgcg acgctcttga gccgtcttga agacgtcgtc
     1981 caagcacacg actcctttat gagccgtcag attcggcgca acggcatctc gctgtttcac
     2041 ggacgagcgc gtttcctgag tgagcacacg atcgagatgc aaaccgtcga cggagtccaa
     2101 caacaattca ccgccgatac catcgtggtc gccacaggct cgcgcccgag aaaccccgag
     2161 gcgatccccg tcgaccatga gcacatcctt gacagcgact cgctcctttc catgatgtac
     2221 ttaccacggt cactggctgt cgtcggaggc ggcgtgatcg gctgcgagta tgcctccatc
     2281 tttgcgctgc tgggagtgga agtcacactg atcgatcgcg cttcctatcc gcttcaattt
     2341 atggatcgag aactggtcga acagttcatc aaagggtttg aacagcaggg cggccattat
     2401 ctcggcggac agcatatcga gaaggtgcga tggaacgagg ttgcacacgt cgtcacgaaa
     2461 ctgagcgatg gaacgacaat caataccgag aagatgctgg tcgcgctagg gcggcaggcc
     2521 aacgtcgaag acttgaatct ctctgcggcg gggatcatgg tttccgataa aggcgtgatt
     2581 cccgtgagtc aatactgcca gacgaatgtt ccccacatct atgccgttgg cgatatggtc
     2641 ggggcgccgg cattggcgtc caaagcgatg gagcacggcc gccgtgccgt ccgccacgcc
     2701 ctcaatctcc cgatcggtga tgcagcctcg accatcccgt taggcatcta tacgattccg
     2761 gaaatggcga gtatcggcct tgatgagatg gctgcccggg agcgctatcg agatccattg
     2821 atcggtcggg caaagtatga agaagttgct cgagcgcaga tatcaggggc aagtcatggg
     2881 cttctcaaga tggtggccga tccagccggt gagcgactac tcggggtgca agtcgttgga
     2941 gattccgcca cggaactggt gcacctggga caattagccc ttcagagcgg cgcgacgatc
     3001 gaatcgttta tcgacaacgt cttcaacttt ccgacctatg ccgaagcgta ccggatcgct
     3061 gcactcgata ttcttggaca ggcagcgaag attcgaacgg ccaaagctgc atagcggtga
     3121 gcatacctgg aaacgatgcg tctattcgcc gttagccctt tatattgccg atcggtcgga
     3181 gttccgcgac tgttttggta attcccgcac gatcgaccgc ctcgaccacc tccgagatat
     3241 tcttataagc gaaccccgcc tcctcagcca gcccagacat tgagacagct ttcacgagga
     3301 taccgcgctg tttcatctgt ttctgtatct gttcaccacg aacagtcttc ttcgcctggg
     3361 atctcgacat cgtacgtccc gacccatgca ttgtcgaagc aaaggtatcc cgcaccgctc
     3421 cttccgtccc caccaacaga tacgatccgg tttccattga accgccgcaa atcaccggtt
     3481 gacccgtcat gcgataccgc ggcgagagct cgagacttcc tggacccaat gctctcgttg
     3541 agcctttccg atggaccacc agttctcctt ccggataccg ctcaactttg gcgatattgt
     3601 gcgccacgtc gtagaccagt tccataccca actcttctgc cggccggctg aatacagcgg
     3661 caaacgcttc tctaatctga tgagtgatga cttgacgatt ggcgaaagcg gtattggccg
     3721 cacaattcat agccgagaaa taatcctgcc cttcgaccga tgtaaacgga gcgcaggcga
     3781 gttgctgatc tttcacggag atcccatatc gccgcatcgc tcgttcgaat accttgagat
     3841 aatcactcgc gacctggtgg ccgaaccctc gtgacccgca gtgaaccatg acaacaatct
     3901 gatcttggcc tgcgatcccc cacgctgtgg cagtttcttt gtcaaatacc cgatcactgg
     3961 ataccacctg tacctcgaga tagtggttac ctgaccccaa ggtccccaac tgattgattc
     4021 ctcgactgac tgcctggtcg ctgattcttg aagggtcggc tccggttata catcctcctt
     4081 cttcaattgc attcaaatcg cgatgccaac cgaatccctg ctcgatacac catcgagccc
     4141 cattagtcat aacttgttca aatgaggagc gattcaaccg tacaaaccct cctgcaccca
     4201 cccctgccgg cacccgacga aacaattccg tcatcaatcg atcaagatgt ggctgtacat
     4261 ctttccggat gagatctgtt ctgatgaggc gcatgccaca attgatgtca tagccgacac
     4321 ctccaggcga aatgactccc gaccggacat cgaatgcggc gacacctccg atgggaaaac
     4381 catagcccca atggccatcc ggcatacaga gcgcatatcg tctaattcca gggaggcaag
     4441 caacgttcgt aacctgctcg aacactccgg catccatcga ttgaagaata gccggtgtgg
     4501 cataaacccg tgcgggtaca agcataccgg atttttcggt aacgggaatt tcccatacct
     4561 cgtcactaat ccgattgacc cgcatttcgg tattcagctt catacgtcca acaccaccct
     4621 cacggcccat tgtcctccct ct
//
LOCUS       DATMTI010000165         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_176671, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000165 DATMTI010000000
VERSION     DATMTI010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176671"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VN039_02760"
     CDS             complement(<1..429)
                     /locus_tag="VN039_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HWV44940.1"
                     /translation="MKQILMVGAGSVGGFFGARLAKENPNVSFLLRPNTLAAVKQNGL
                     TIRSADGTFTVKPHAASDARQLPRPDLIVLGVKAYDLDEVLSQIEPVLTEKTVVLTLQ
                     NGIDTEDRLLARIQRDCIVGGVAYIYSKIVEPGVIEHYKKG"
     gene            520..1254
                     /locus_tag="VN039_02765"
     CDS             520..1254
                     /locus_tag="VN039_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HWV44941.1"
                     /translation="MAPGSDLGIVRQQLSAARSITVLTGAGISADSGVPTFRGTDGLW
                     KNFRAEDLATPEAFERDPRLVWEWYNWRRELIVTKQPNSAHKAIVELERRCSDFWLIT
                     QNVDGLHRDAGSQKLSEIHGNIWKVRCTGCGVVEHNRDVPIAILPSCAHCGSLLRPHI
                     VWFGESLFTDDLDCCSNALNRCDVLLIIGTSGVVYPAAGFASVAKEGRAFVAEINLAP
                     TPQPALVDISLQGRAKDLVPLLLDPV"
     gene            complement(1234..>1827)
                     /locus_tag="VN039_02770"
     CDS             complement(1234..>1827)
                     /locus_tag="VN039_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWV44942.1"
                     /translation="IKQAFSRAFREAPPLVFAAAEPARIKQSERLWWFDIVHHVFYRV
                     GMFERFDEFFDHVFRVFEDDRSWRLFPETASTLTRLKARNLELGIISNFDSRLFTVLR
                     GLGIADAFDTVTISSLAQAAKPAPQMFHIALEKHAVDPEEALHVGDSLRDDVEGAEKA
                     GLQAVLLDRDGSQQEASGVQIIRSLEGLFPFLDRIEQ"
BASE COUNT          379 a          535 c          502 g          411 t
ORIGIN      
        1 ccctttttta taatgctcga tcacgccagg ctcgacgatt ttggagtaga tataggcgac
       61 gccaccgact atacaatctc gttggattcg cgcgagaagc cggtcttcgg tatcgatccc
      121 gttctgtaag gtaagaacca cggttttttc ggtcagcacc ggttcgatct ggctcaggac
      181 ttcgtcgaga tcataggctt tcacgccgag cacgatgaga tccggccgag ggagctgacg
      241 cgcatccgag gctgcatggg gcttgaccgt aaaggttcca tcggcgctgc ggatcgtcaa
      301 tccgttttgt ttcaccgctg ccagcgtgtt cggtcgcagt aaaaacgaca cgttgggatt
      361 ttccttggcc aatcgcgctc cgaaaaatcc gccgactgag cctgcgccga ccatcaagat
      421 ctgtttcatg ttctcatcct catggttgcc tgtgaaacgg tcgtactata gcatgcggtt
      481 gcgcggcaac gcagcgcact tataggagat cggtatatca tggcgcccgg atctgatctt
      541 ggaattgtga gacaacaact ctctgctgcc cgaagcatca ccgtgctcac cggtgcaggg
      601 atctcggccg acagcggtgt gccgacgttt cgcggaacgg acggcttgtg gaagaacttt
      661 cgcgccgaag atctggcgac gcccgaggcc ttcgagcggg atcctcgcct cgtgtgggag
      721 tggtacaact ggcggcgcga gctgatcgtc acaaaacagc cgaactccgc ccacaaagcc
      781 atcgtcgaac tggagcgtcg ctgttcagac ttttggttga ttactcaaaa tgtggacgga
      841 ttgcaccgag atgccggttc acagaaactg tctgagattc acggcaacat ctggaaagtt
      901 cgctgcaccg gctgtggcgt ggtggagcac aaccgcgatg tgccgattgc catcctcccg
      961 tcctgtgccc attgcgggtc gctgctccgg ccccatattg tctggttcgg agaatccttg
     1021 tttaccgatg acctcgactg ctgctccaat gcgctcaacc gctgcgacgt cttgctcatc
     1081 atcggcacat ccggggtcgt ctacccggct gccgggttcg catccgttgc caaagaaggc
     1141 cgtgcattcg tcgccgaaat caatcttgcc cccaccccac agccggcgct ggtcgacatc
     1201 tcactgcagg gccgcgcgaa agatctcgtg ccgttattgc tcgatccggt ctaggaaggg
     1261 gaacagccct tccaagcttc tgataatctg aacgccgctt gcttcttgct gactcccgtc
     1321 acgatccaac aggacggcct gaagaccggc tttctccgca ccttccacgt cgtctcgcag
     1381 actatccccg acgtgcaagg cttcttccgg atcgaccgca tgtttctcca acgcgatgtg
     1441 gaacatctgt ggggctggtt ttgcggcttg ggccaaactc gagatcgtca ccgtgtcgaa
     1501 tgcatcggca atcccgagtc cacgcaacac ggtaaagagt cgggaatcga aattcgagat
     1561 gatcccgagt tccagatttc gtgctttgag ccgagtcaag gtggaagcgg tttcaggaaa
     1621 caatcgccat gatcggtcgt cctcaaacac acgaaacacg tgatcgaaga actcgtcgaa
     1681 tcgctcgaac atgcccacac gatagaagac atgatgaacg atatcgaacc accacagccg
     1741 ttcactctgt ttgattcgcg ccggttcagc cgctgcgaag accagcggag gggcttcgcg
     1801 gaacgctcgg ctgaacgctt gtttgat
//
LOCUS       DATMTI010000166         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_388394, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000166 DATMTI010000000
VERSION     DATMTI010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388394"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..>2118
                     /locus_tag="VN039_02775"
     CDS             73..>2118
                     /locus_tag="VN039_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV44943.1"
                     /translation="MAGWGHRVAGIAEDITDHRQQLALLAQTEAVGKIGGWEIDFLTD
                     HLWWSDETYRLHDTTPELYSPTVDTAMNFYTPESRPVIADALQKGVKQGEAWDLELEL
                     ITAKRRRIAIRVVGKVDVVNGRTVRAYGTFQDITERKRAEEALRKVHDELECKVVERT
                     AELQASEERYTRATAIGKVGVWELDVLTGQYHGDANLKALFGYTPEELSTDPYAWLNL
                     VHPDDQPIAMKNWELVQCGAADACHYELRHIRKDGSVVWGDVRAHAVRDQNGRLTNLI
                     GATVDITERKRIEDALRDSEERFAKAFRTSPHPIGITEMATGRCIEVNDACLELFGFR
                     REEVIGNTTLMLGVWPNPEDRARLVERLKAGEPVRNKEFALRTKSGPLRYLLTSCDLV
                     ELNGTPCLITVGSDITERKRAEEALRISEERFAKAFQAGPHPVVISEVDSGRVLDTND
                     AACQLFGYHKEEVVGRTTLQIGLWPSTQERDRFVEQLKSSGSVHNMEVTLQRKDGDVR
                     QCLLSSELIELNGKPCSVTVGDDITERKRMERALRRTQFSVDQAVEAILWVDQTGRIF
                     NVNETACRMLGYTRQDLTTITVHDIDPNFPVERWPEHWGHVKEQGALAFEAKFCSRTG
                     GVLETDVTVNYLQYEGKEYGCMILRDIGERKRAEAERRRSHTFLRQVIDTDPDFIFAK
                     "
BASE COUNT          513 a          493 c          669 g          443 t
ORIGIN      
        1 gagtttgact gcgagtaccg gattgtggat cgggacggac gtatccggtg gatttccgac
       61 cgtagagtca aaatggccgg gtggggccat cgtgtcgccg gcattgcgga agatattacc
      121 gaccatcgac agcagcttgc cctgctggcc caaacagaag ctgttggaaa aatcggagga
      181 tgggaaatcg attttctgac cgaccacctc tggtggagtg acgaaacata ccggttgcat
      241 gatacgacac cggagctgta cagcccgacg gtggacacgg cgatgaattt ctatacaccc
      301 gagagtcgac ccgttattgc cgatgcactt cagaaagggg tgaaacaggg agaagcgtgg
      361 gatctcgagt tggaactgat cacggcgaag aggcgccgga ttgccatacg ggtcgttggg
      421 aaggtcgatg tggtgaacgg acggacggtt cgggcgtacg gcacgttcca agacattacc
      481 gagcgcaaac gggccgaaga ggctcttcga aaggtccacg atgaattgga gtgcaaagtc
      541 gttgagcgta ccgctgagtt gcaggccagc gaagaacgtt atacgcgtgc gactgctatc
      601 ggtaaggttg gagtctggga actggatgtc ctaacaggtc aataccatgg ggacgcgaat
      661 ctgaaagctc tctttgggta tacgccggaa gaactctcca cggatcccta tgcttggctg
      721 aatctggtcc atccggatga tcagccgatc gcgatgaaga attgggaact cgtgcagtgt
      781 ggagcagccg atgcatgtca ctatgagctc cgtcacataa ggaaagacgg ctcggttgtt
      841 tggggtgatg tgcgagccca tgctgtccga gatcaaaacg gacgattgac caacttgatc
      901 ggcgccaccg tcgatatcac tgagcgtaag cggatagaag acgcgttgag agatagtgaa
      961 gagcgatttg ccaaagcttt ccgcacgagc ccgcatccaa tcggtatcac ggaaatggcc
     1021 acgggccgtt gtatcgaggt gaacgatgcc tgtttggagc tgttcggttt tcggcgagag
     1081 gaagtgatcg ggaacaccac attgatgttg ggtgtctggc cgaatcccga agatcgagcg
     1141 cggctcgtcg agcggctgaa agccggagaa ccggtgcgca ataaggagtt tgctttaagg
     1201 accaagtccg gcccattgcg ttaccttctg acttcgtgtg accttgtgga gctgaatggc
     1261 acaccgtgtc tcattactgt cgggagcgac atcaccgaga gaaagagagc agaggaagcg
     1321 ctacgaatca gtgaggagcg ttttgccaag gcgttccagg ctggtcccca tccggtcgtc
     1381 attagtgaag tggactccgg tcgagtgttg gacaccaatg atgccgcttg ccagctgttc
     1441 ggatatcaca aggaagaggt ggtgggacgc acgacattac agattgggct ctggccctcg
     1501 acgcaggaac gggatcgttt cgtcgaacag ctgaaaagtt ccggatcagt tcacaatatg
     1561 gaggtgacgc tccagaggaa ggatggcgat gtccgtcaat gcctgttgtc gtcggaactc
     1621 attgagttga atggcaagcc atgctctgtg acggtcggag acgatatcac ggagcgcaaa
     1681 cgcatggaac gagcgctacg gcggactcag ttctcggtgg atcaggcagt ggaggcaatt
     1741 ttatgggtcg accagaccgg tcgaatattc aacgtcaatg aaaccgcatg tcggatgttg
     1801 gggtatacgc gccaggacct gacgaccata accgtgcatg atatcgatcc taatttccct
     1861 gtggaacgat ggccggagca ttggggtcat gtaaaggagc agggagcgct ggcgttcgaa
     1921 gcgaagtttt gctcacgaac cggaggcgtc ttggaaacgg atgtgaccgt caattatctg
     1981 cagtacgagg ggaaagagta cggctgtatg atcttacggg atattggaga acgcaaacgg
     2041 gcggaagccg aacgtcgacg ctcccatacg ttcttgcgac aggtcattga cacagacccc
     2101 gacttcattt ttgccaag
//
LOCUS       DATMTI010000167         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_388486, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000167 DATMTI010000000
VERSION     DATMTI010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388486"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..867)
                     /locus_tag="VN039_02780"
     CDS             complement(202..867)
                     /locus_tag="VN039_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV44944.1"
                     /translation="MVVMGIVLLSNQGCNTKGLKSESEGERGGNIVSTLPDPLSGGNS
                     SGDTMLPTISGEHSSGELSGFSRSPSEERITQGGYGGALTPSEVGPRQRAELTKDDKA
                     AVEAGLQDVFFGYDQWTLSDAGMDALNHSATYLNSHPAAVLKIEGHCDERGTAAYNMV
                     LGDNRAKAARNYLADVLVKLRQLAIVSYGKERPFCLDRDESCYQQNRRDHMLLSINDE
                     GIP"
     gene            complement(1180..>1542)
                     /locus_tag="VN039_02785"
     CDS             complement(1180..>1542)
                     /locus_tag="VN039_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44945.1"
                     /translation="DQAGVVLGAAAEPEPRRACRRRFLVEMIMADLLAARSTIDGYRD
                     VIVSVGAHDPTTRHILEQILAQEEEQPKALAPLLRVLSPHRNRLVQALQRYPRHRVDL
                     RRTTHQMTGRVTSLPGIS"
BASE COUNT          348 a          446 c          388 g          360 t
ORIGIN      
        1 gatcaagagg taggcgaaga aacgatgaaa ccagatcgga cacgatagtg ctgccacaat
       61 ttagccacag cgaaaagtat tagggctact cggcgtaaat ttgggttgac gacctttccg
      121 gtccgtacga cctacaatca atgtacccag tttgaagaat ttccccgggt tatgagcgcg
      181 ctccacgcgg atgacgcctc atcatggaat cccttcatca ttgatggaaa gaagcatgtg
      241 gtcgcgtcgg ttctgctgat agcaggattc gtcccgatca agacagaagg gacgctcttt
      301 accataggag acgatggcta gctgcctgag cttcacgaga acgtccgcaa gatagttccg
      361 tgcagccttg gcgcggttgt cgcccaaaac catattatag gcggcggtgc ctcgctcgtc
      421 acagtgccct tcaattttca gcacagccgc tggatgactg ttgagatagg ttgcactatg
      481 attgagcgcg tccataccag catcagacag ggtccattga tcatagccaa agaaaacgtc
      541 ctgtaaaccc gcctccacgg cagccttgtc atcctttgtc agttcggcac gttgacgagg
      601 gccgacctct gatggggtca gtgctccccc atatccgcct tgtgtaattc gttcctccga
      661 cggagaacga gagaacccgc tcaattcacc gcttgagtgt tctccggata tagtgggcag
      721 catcgtatca ccgcttgagt ttcctccgga tagtggatca ggaagtgtgg atacgatgtt
      781 gcccccgcgt tcgccttcag attccgactt gagccctttt gtattgcacc cttggttcga
      841 taataaaaca atccccatca ccaccattac cgctccagat tttgctagtg ctctattcat
      901 aacctctccc ttcggatgac atccacgata gataaaaaga cgcttgtgtg tacgactcct
      961 accatcatgt cctgagggtc ggccggatca gccgcgctcc ccatgctacg gaggtgattc
     1021 gcgcgcacga cggcatcctc gatatcccca caattgatgc agcgtgtggc cgggcataga
     1081 gcatttgtct tgacgttcca ttcctcgaag ctttctcgta ccaatagccc acggcagcat
     1141 tgacaaagca tatctcctcc ttcagcctca aacgtgcgcc tatgagatcc caggcaaact
     1201 cgtgacccga cctgtcatct gatgagttgt gcggcggaga tcaacccgat gacgtgggta
     1261 tctctgcaaa gcctgaacga gacggtttcg gtggggactc agcaccctca agagcggagc
     1321 cagtgccttg ggctgctctt cttcctgcgc gagaatctgt tccaagatat gtcgagtcgt
     1381 cggatcatgg gccccgacag acacgatcac atcgcgatag ccgtcgatcg tgctccgtgc
     1441 agccagcaga tccgccatga tcatctccac caggaatctc cttcgacatg ctctgcgtgg
     1501 ctcgggttca gcagccgccc caaggacaac tccagcttgg tc
//
LOCUS       DATMTI010000168         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_388521, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000168 DATMTI010000000
VERSION     DATMTI010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388521"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VN039_02790"
     CDS             complement(<1..905)
                     /locus_tag="VN039_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWV44946.1"
                     /translation="MAEQRSLKFYQADVFTSQPFGGNPVAVFPDSDGLTDDELQQIAR
                     EMNLSETVFVFPPTDPAAVARLRIFTPTQEIPFAGHPVLGTFYVLAQLGRVSTRGQVT
                     HVMQECNIGLFPVELHAEQSRVVRVVMSQPKPEFLDPLDAIDDVYLVGGALGLPKHVI
                     ADMKWPLQVVSTGLPVLIVPVRTLTAVRSINPDASAIINVCERFGANGIMVFTTVTVE
                     SFASVHARMFAPKIGILEDPATGSAGGALGAYLVQNGVVEVGPRTDILIEQGYEIDRP
                     SRILVQVESDDDVIQGVKVGGQCVMV"
     gene            1012..1278
                     /locus_tag="VN039_02795"
     CDS             1012..1278
                     /locus_tag="VN039_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44947.1"
                     /translation="MDHITEQDVAHALEILGLTLPITTEALERAKRVQLYNWTPTRYA
                     GLSNNPKQYMQQFRKAEEMTRTVEAAYALLSAVFVPDTEDSDRS"
     gene            complement(1358..>3301)
                     /locus_tag="VN039_02800"
     CDS             complement(1358..>3301)
                     /locus_tag="VN039_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWV44948.1"
                     /translation="NGLLQIDGLTPITKEDRKKSKVMLIFPPEWVPTAPYLALPSLTA
                     VLREAGHTVIQRDINIGMWDHFFSMEFLIWVKARLGMQLKALQEKEKAGVFSERDVTH
                     MAVVEQAYALDVFDLADRAEDAKLIVRGERFYEAETLEGALNTFRETMVYISAAYYPA
                     SLVFYPMESNLGYRPGVSKEVFACLEDEQVNVYRDLCNQLVMPDVAREQPHVVGISIG
                     TQMQLLAGLTFARMIKEAFPEVHVVVGGNIITRLQEDLSNHERFFREIFDSAILYEGE
                     HALLWLIEALNDQRPLASVPNLIYRDESGLHRNPEIYTEKTTALPLPDFEGMPLDRYF
                     VPELIIPYLATRGCYWGRCTFCDHGQGYFDQYRGMPAQLVIDQIKALRDKYHCRHFLF
                     SDESYPPALFKKVSQLLIDQDVGIKWTTLIRFEETLQDQATWDLAAKAGCCTLYYGME
                     SANERVLNLMDKHARKSVIERNLQMAAKAGIWNHVMAFYGFPGETFEEAMETRQFVID
                     HQPVIHSLELFYFVAYRHTPMVRHPDKFGITIHKQEEYDLPLDYYYTLNDPSTLSCLD
                     AMQLCEEFYKHDFHPWAVRVNSREHVFLYISKFGSNQLPQIYAQQTQPVGSPDGVSGL
                     ITWPVAHAEGDEGMSRVTSHEIG"
BASE COUNT          792 a          981 c          828 g          700 t
ORIGIN      
        1 accatcacac actgcccgcc gaccttcaca ccttggatca cgtcgtcgtc cgactcaact
       61 tgcaccagaa tccgtgacgg ccgatcgatt tcgtagccct gttcaatcag aatatccgtc
      121 ctcggtccga cttcgacgac gccgttctga accaggtacg ctccaagcgc ccccccggcg
      181 ctccccgtcg caggatcctc caaaattcca attttcggag caaacatcct cgcgtggaca
      241 gaagcaaacg attcgaccgt taccgtcgtg aagaccataa tcccgttggc cccgaatcgt
      301 tcgcagacat tgatgatggc cgacgcatca gggttgatcg accggacggc cgtcaacgtt
      361 cgcaccggca cgatcaagac cggtaagccg gtcgacacga cttggagggg ccacttcatg
      421 tcggcaatca cgtgcttcgg caagccgaga gcgccaccga ccaagtacac atcgtcgatg
      481 gcgtcgagcg gatcgagaaa ttccggtttg ggttgagaca tgacgacccg caccacacga
      541 ctctgttccg catgcaattc gacggggaat agaccgatat tacattcctg catgacatgc
      601 gtcacctgtc cccgagtaga aaccctcccg agctgggcca acacgtaaaa tgtgcccaag
      661 accggatggc cggcaaaggg aatttcctga gtcggggtaa agatccgcaa ccgagcaact
      721 gcagcaggat cggtcggtgg aaagacgaac actgtttccg atagattcat ctcccgcgca
      781 atctgttgga gctcgtcgtc tgtcaggcca tccgagtcgg ggaaaaccgc gacggggttg
      841 ccgccgaacg gctgagaggt aaagacgtca gcctgataga atttgagaga ccgttgttca
      901 gccatggtca ctccaaaact cgtccggata acacctgacg atccgatcat gccatgagaa
      961 aataatcgtc atcatttgac aggtcttgcc cctctttggc agactccgcc catggaccac
     1021 atcaccgaac aggacgtcgc ccatgcattg gaaattcttg gccttacgct tcccatcacg
     1081 accgaggccc tggaacgggc gaagcgtgtc caactctaca actggacccc cacgcgttat
     1141 gccggtctca gcaacaatcc gaagcaatac atgcaacagt ttcgaaaagc cgaagaaatg
     1201 acccggacgg ttgaagccgc ctacgcatta ttgtcggctg tattcgttcc ggatacggaa
     1261 gactcagatc gaagttgagg ttaagcactc ccttcagaac ttaaccttat cctcaacctc
     1321 agctttaatc tgattcctcg ctcaacccca accttcttca cccgatctcg tgactcgtca
     1381 cgcgagacat gccctcgtct ccctccgcat gagcgaccgg ccaggtaatc aagcctgaca
     1441 ctccgtccgg cgaccccacc ggctgcgtct gctgagcata aatttgcggc aactggttcg
     1501 acccgaactt cgagatatag aggaacacat gctcgcgcga gttcacccgc acggcccagg
     1561 gatgaaaatc atgcttgtag aattcctcgc agagctgcat cgcatccagg cacgacagcg
     1621 tactgggatc attcagcgtg tagtaatagt ccagcggcag atcatactcc tcctgcttgt
     1681 ggatcgtaat gccgaacttg tcgggatgac gcaccattgg ggtgtggcgg taggccacga
     1741 agtagaagag ttccagcgag tgaatcaccg gctggtgatc gatgacaaac tgacgagttt
     1801 ccatggcctc ttcgaacgtc tcacctggaa atccataaaa agccatcaca tggttccaaa
     1861 tccccgcctt cgccgccatc tgaagattgc gttcgatgac gcttttccga gcgtgcttgt
     1921 ccatgagatt caagacccgc tcattcgctg actccatccc ataatagagc gtacagcagc
     1981 ctgccttggc cgcgaggtcc caggtggcct gatcttgaag cgtttcttca aaccgaatga
     2041 gcgtcgtcca cttgatcccg acgtcttgat caatcaacag ctgggagact ttcttgaaga
     2101 gggccggtgg ataggattca tcggaaaata gaaagtgccg acagtgatat ttgtcgcgta
     2161 acgccttgat ctggtcaatc accagctgag ccggcattcc acggtattga tcgaagtaac
     2221 cttgaccatg gtcacagaac gtgcagcggc cccaatagca gccgcgcgtg gccaggtagg
     2281 gaatgatcag ctccggaacg aagtagcgat ccagcggcat gccctcaaaa tccggcaacg
     2341 gtagcgcggt cgtcttctcg gtgtagattt ccggattccg atggaggccc gactcatcgc
     2401 gatagattaa gtttggaacc gaggcgagag gccgctgatc attcaacgct tcgatgagcc
     2461 acagcaaggc atgttcgccc tcgtagagaa tcgccgagtc gaatatctct ctgaagaacc
     2521 gctcgtgatt cgagagatct tcctgcagcc tggtgatgat gttgcccccg accacgacat
     2581 gaacctctgg aaacgcttcc ttgatcatcc gagcgaacgt caatcccgcc aacaactgca
     2641 tctgcgtccc aatggaaatg ccgaccacgt gaggctgctc tctcgcaacg tccggcatga
     2701 ccaactgatt gcagagatcc cgatagacgt tcacctgttc atcttccaaa caggcgaaca
     2761 cttctttgga gacaccgggg cgatagccta aattactttc catcggatag aagacgagtg
     2821 atgcgggata ataagcggcg gagatataga ccatcgtttc acggaaggtg ttgagcgccc
     2881 cttccagcgt ctccgcctca tagaaacgct cgcctcgcac aatcaacttg gcatcttcgg
     2941 ctcgatccgc cagatcaaag acatcgaggg cataggcttg ttccacaacc gccatgtgag
     3001 tcacgtctcg ctcactgaag actcctgcct tctctttctc ttgcaaagcc ttcagctgca
     3061 tgcctaaccg agccttcacc cagatcagaa actccatgct gaagaagtga tcccacattc
     3121 cgatgttgat atcgcgctgg atgaccgtat ggccggcctc acgcaagacg gctgtcagcg
     3181 aaggcaaggc aaggtatggc gccgtcggta cccactcggg cggaaagatc agcatgacct
     3241 tcgatttttt ccgatcttct ttcgtgatcg gggtcaagcc atcaatctgt agaagtccat
     3301 t
//
LOCUS       DATMTI010000169         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_813125, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000169 DATMTI010000000
VERSION     DATMTI010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813125"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VN039_02805"
     CDS             <1..225
                     /locus_tag="VN039_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWV44949.1"
                     /translation="IVDELAKEYAGKLKVRKLNTDENPEVAGRYQVMSIPTILFFKNG
                     QPVEKLVGARPKRQFKEMIDSLLAQHAGTV"
     gene            484..2163
                     /gene="recN"
                     /locus_tag="VN039_02810"
     CDS             484..2163
                     /gene="recN"
                     /locus_tag="VN039_02810"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWV44950.1"
                     /translation="MLTELRITNFAVIERLSLTIDSGFTVLTGETGTGKSLLIDAVAL
                     LMGGRASSDQIRFGEDEAQLEASFEIPLTHPLLQRLRDGEILGPQDSQLIIRRIIARS
                     GRNRVYLNGVLSPVHVLEEFAGTLIDIHGQHDQQSLLSNSAQLEVLDAFGRLLELRSQ
                     YRSTHRDWVRFREERAALAARLQQGARQEDLLRFQRQELDEAGCRIGEEELLQAERRR
                     LGSSRRLAELVAEAQERIQDDTHGILANLVSTERVLGELSQIDAEMQDTVRFVSEAKV
                     LLKEVADSLRGYAEGLDADPMRLSVIDDRLTVIQKMKKKYGGTIEAVLETHNRVKHEL
                     EQLRGADGELDRYDRLIEEQQRNVSALARTLSEKRADAAQQLTKLAGKELNALKMGSV
                     RFLVQVLPSGPDEIYGPDGADRVEFLLSANAGEPLKPMPRVASGGELSRVMLALKSVL
                     ADVDHVPVLIFDEIDTGVGGAVAATIGKRLRELGRYHQVLCITHLPQVASQAQHHFSV
                     EKSEVKGRTMATVRSLTGMSREGEIARMLGGERITQKTRSAAAELIAGAPE"
     gene            complement(2165..>2829)
                     /locus_tag="VN039_02815"
     CDS             complement(2165..>2829)
                     /locus_tag="VN039_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWV44951.1"
                     /translation="RAWDMVKTGYTSFFNAQDNPALQDDMNLLPLLSDILEATDRQLL
                     NHSSRVSFHATLMASRLNLPASEQKSLALGAFLHDIGKTTLPSYRFSEDQVLPSGEAS
                     LCREHSSRGARMLAPFGLPIEVRQIVASHHERWDGQGYPHGLRGTEIPLSARIVGIAQ
                     TFDHLTADTPGRVALPLDVAIRRISLQSHTHFDPLLLEFFIQVVKDSSVSPPAMDIAP
                     AA"
BASE COUNT          633 a          711 c          875 g          610 t
ORIGIN      
        1 atcgtcgacg agctggcaaa agaatatgcc gggaagctga aggttcgaaa actgaacacc
       61 gatgaaaatc cagaggttgc gggtcgttac caggtgatga gtatccccac gattcttttc
      121 ttcaaaaacg gccagccggt cgagaagctg gtgggtgcca gaccaaaacg ccagttcaaa
      181 gagatgatcg actcactgct tgctcagcac gccgggactg tctaaataag atgctgaagg
      241 cggcagccgg ccctcgtgct cgcgagacac agccgcctca ccatctcggt ggcattcaga
      301 ggtgtgccgt gctttatccg gcacggcgtg aacctcaggg gttcgacgta cgcggggcgg
      361 gcgagatcag cagccgtttt gagcatcttg tgtgttgttc catctggttt tagtcggcac
      421 tggctcacgt tgagctccat atgtgtctcc tgcagttagc cgggtctcgc cacagttgca
      481 gccatgctca ctgagctgcg tatcacaaat tttgccgtga tcgaacggct gagtctgacg
      541 attgactcag ggtttaccgt gttgaccgga gagacgggga ccggtaagtc tttgttgatc
      601 gatgcggtgg ctcttcttat gggcggacgg gcctcaagcg accagattcg attcggcgaa
      661 gacgaagctc agcttgaagc gtccttcgaa attccactca cacatcccct ccttcaacgg
      721 ctgcgggacg gagagatcct tggaccgcag gattcccagc tcatcattcg acgcatcatt
      781 gctcgttcag gaagaaaccg ggtttacctg aatggagtct taagtccggt ccacgtgttg
      841 gaagagttcg cagggacgct catcgatatc catggccagc acgaccagca atcgctcttg
      901 tcgaactccg ctcaacttga ggtactggat gccttcggcc gtctgctgga attgcggtcc
      961 cagtatcgat ctacccatcg tgattgggta cgttttcgtg aagaacgcgc tgcgcttgcc
     1021 gcgaggttac aacagggggc caggcaggaa gacttgttgc gttttcaacg gcaggaattg
     1081 gatgaggctg gttgccgtat cggagaagaa gagttgctgc aagccgaacg ccgtcggttg
     1141 gggtcgtcgc ggcgtctggc agagttggta gcagaagctc aggaacgaat ccaggacgat
     1201 actcacggta tcttggcgaa tctggtgtcc acggagcgtg tgttgggaga actctctcag
     1261 attgacgccg agatgcaaga caccgttcga tttgtatccg aggccaaagt gctcctgaaa
     1321 gaagtcgccg attcccttcg tggttatgcc gagggcttgg atgccgaccc tatgcgattg
     1381 agtgtgatcg atgatcgttt gactgtcatc cagaagatga aaaagaaata cggagggacg
     1441 atcgaagccg tcctggagac gcataatcga gtgaaacacg aactggagca acttcggggg
     1501 gcggacggcg agctcgatcg gtacgatcgt ctcattgagg agcaacaacg gaacgtgtcg
     1561 gcgctggccc gaacgctctc agaaaagcga gcggacgcag ctcaacagtt gacgaaactg
     1621 gcgggcaaag agctcaacgc actgaaaatg ggatccgtac gatttctggt ccaagtcctg
     1681 ccgagcgggc ctgatgaaat ctacggtccg gatggggccg atcgtgtcga gtttttgttg
     1741 tccgccaatg ccggcgagcc gctgaaaccg atgcctcgtg tggcatccgg cggcgaacta
     1801 tcgcgggtta tgttggcatt gaaatccgtc cttgctgatg tcgaccatgt gccggtcttg
     1861 atcttcgatg agatcgatac tggcgttgga ggggcggtcg cggccacgat cgggaaacgg
     1921 ctcagggagt tgggccgata ccatcaagta ttgtgcatca cgcatctccc gcaggtcgcc
     1981 tctcaagccc agcatcattt ctccgtcgaa aagtcggaag tgaagggacg gacgatggcg
     2041 acggtgcgtt cgttgaccgg catgagtcgc gagggagaaa ttgcgcgcat gctcggtggg
     2101 gagcgaatca ctcaaaaaac acgatctgcg gcggcagagc tgatcgccgg agcgcctgaa
     2161 tagatcaagc ggcgggagcg atgtccatgg cgggaggcga aacggacgag tccttcacga
     2221 cttgaatgaa gaattccagc aacaacgggt cgaaatgcgt atgagattga agcgagatcc
     2281 ggcggattgc gacatccagc gggagggcga ctcgcccagg tgtatcggcg gtcaaatgat
     2341 cgaatgtttg ggcgatgccg acgatgcggg ccgataacgg aatctccgtg cctcggagtc
     2401 cgtgagggta gccttgccca tcccagcgct catgatggga tgcgacaatt tgcctcacct
     2461 caatcggtaa accgaacggc gcgagcatcc gtgcgcctct gctggaatgt tccctacaga
     2521 gggatgcttc gccggacgga aggacctgat cttctgaaaa cctgtacgat gggagggtgg
     2581 ttttccctat gtcatgcaaa aaggccccca aggccaatga tttttgctcg gaggcgggga
     2641 gattgagtcg gcttgccatc aacgtggcat ggaaactcac tcggctcgaa tgattgagta
     2701 attggcggtc ggtggcttcc agaatatcgg ataagagtgg aagcagattc atatcgtctt
     2761 gcaaggcggg attatcttgc gcgttgaaga acgaagtgta tcctgtcttg accatgtccc
     2821 acgctcgtg
//
LOCUS       DATMTI010000170         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_91514, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000170 DATMTI010000000
VERSION     DATMTI010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91514"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VN039_02820"
     CDS             <1..269
                     /locus_tag="VN039_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44952.1"
                     /translation="EMLTEKFLTYFHPLTGGSEGLGWPFGEKIYFSEIYRLILTTEDV
                     ARVNADDVTIFSDGSRVTECSDIDLAEDEVVYSSDHKVEVSYNA"
     gene            269..>2028
                     /locus_tag="VN039_02825"
     CDS             269..>2028
                     /locus_tag="VN039_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44953.1"
                     /translation="MATITKQYQFLNPVNGWHASESMRSKLRPRPHDGTLMLPALTGQ
                     ARPLLDPKIVGEPCGSLTGKTAKSSRSKPGSLCCPSAVAAEPCGSSILVIDATLNRVK
                     RIRFRSAEELVTLTNQGQPYLGSRIDMITAFGGSGIEPRKFRHPRGLARLRSGIIAVS
                     DTGNHRIQLFSPSPHVLLQVWGATDTRGKPIIGTEPRQFHSPTAMVTDHESDSLYIAD
                     QCNKRVQHISADGARVQMIGDTVLRRPSRLAIGPRQLLAVVDTVLSCVFLFSPHVPLP
                     GILSRIETPRSVTFDRNGTLYIGTGKGRIVVFHPSNSSPVEYDLIDESSTHIDGEILD
                     LVWVAPDYLIATIRHTVNGSIKQTLHWLRTTEQCATEGHLVIGPLDSRIDHCPWHRIE
                     MQAAIPEGTSLQLATATSDTLHTEPPSAPSQKGFTQNSLPQWSIEASTGEQNPDWLVQ
                     NGRGRYLWLRITARSNGQASPVITWIKTHYPRNSYLQYLPAVYQEDEESRNFLDRFLS
                     LFQREFDTLDRKLDNAWQLFEPGSTTDIFSDRSAEQVDQKRSVPFEQRRLQWLAGWMG
                     LTLNPEWSEVKLREMLKKTA"
BASE COUNT          504 a          593 c          508 g          423 t
ORIGIN      
        1 aggaaatgct cactgaaaag ttcttgacct atttccatcc attaacagga ggatcagaag
       61 ggcttggctg gccattcgga gaaaagatct acttctccga aatctatcga ctcatcctca
      121 ctacagaaga tgtcgcaagg gtaaacgcag atgatgtcac gatcttttct gatggaagtc
      181 gagtcacaga atgctcggac atcgatcttg ccgaagatga agtagtgtat tcctccgatc
      241 ataaagtgga agtgagctac aacgcataat ggcgaccata acgaagcaat accagtttct
      301 caatccggtg aacggctggc atgcgtccga atcaatgcgt tccaagctgc gtccccgtcc
      361 gcacgatgga accttgatgc tccccgctct caccggccag gctcgtccgc tccttgatcc
      421 aaagatcgtg ggcgagccat gtggcagcct gacaggtaag acagccaaga gctcacgctc
      481 caaaccggga tcgttgtgct gcccaagcgc cgttgcggcg gagccatgcg gaagcagcat
      541 tctcgtgatc gacgcgaccc tcaaccgtgt gaaacgcata cgatttcgat ctgcagaaga
      601 gctggttacg cttacgaacc aaggccaacc ttacttaggc agtcgcatcg atatgatcac
      661 ggctttcgga ggatcgggca tcgagcctcg aaagtttaga catccacgcg gcttagcaag
      721 actacgctca ggaatcatcg ccgtttccga taccggcaac catcgcattc aactcttttc
      781 accatctccc catgtgctcc ttcaagtatg gggagcaacg gatacaagag gtaagccaat
      841 catcggaacg gagccaagac agttccattc cccaacggcc atggtcacgg atcacgaatc
      901 cgattctcta tacatcgctg atcaatgcaa caagcgcgtc cagcacatct cagcggacgg
      961 ggctcgggtc cagatgatcg gagacacggt tctaaggcgt ccctcgcgct tggccatcgg
     1021 tccccgccag ctcttggctg tcgtcgatac agtgttgtcc tgcgtcttcc tcttttctcc
     1081 gcatgtgccg cttcccggca tcctcagccg tatcgaaaca cctcgtagcg tgaccttcga
     1141 tcgaaacggc acgctgtaca ttggaacggg gaaggggcga atcgttgtat ttcatccgag
     1201 caacagttcc cctgtggaat atgacctcat cgatgaaagt tcaactcata tcgatggaga
     1261 aattctcgat ctggtgtggg ttgctcctga ctatctcatt gccaccatca gacacaccgt
     1321 aaacggcagc atcaaacaga ccctccattg gctgagaacg acagaacaat gcgctacaga
     1381 aggtcacctt gtcatcggcc ccttggacag ccgaatcgac cactgcccgt ggcatcgcat
     1441 cgagatgcaa gccgcaatcc cggaagggac cagcttgcaa ctcgccaccg cgacgtcgga
     1501 taccttgcat accgagcctc cttctgcccc ttctcaaaag gggttcacac aaaactcgct
     1561 ccctcaatgg agcatcgaag cgtcgactgg agaacagaat ccggattggc tcgttcagaa
     1621 cgggcgagga cggtacctct ggctgcgaat caccgcgagg tccaatgggc aggcctctcc
     1681 ggtgatcaca tggatcaaga ctcactatcc gcgcaattcc tacctgcaat accttcccgc
     1741 cgtgtaccag gaggacgagg aaagtcggaa cttcttagac cgattcctct cactgtttca
     1801 gcgcgaattt gacaccctcg accggaagct cgacaacgca tggcaactat tcgagcccgg
     1861 ttccaccacc gacatctttt cagaccgatc ggcggagcag gtggaccaaa agagatcggt
     1921 gccgttcgag cagagaagac tccaatggct tgccggatgg atgggcctca cgcttaatcc
     1981 ggaatggtcc gaagtcaagc tcagagagat gctcaaaaaa acggcgag
//
LOCUS       DATMTI010000171         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_388618, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000171 DATMTI010000000
VERSION     DATMTI010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388618"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VN039_02830"
     CDS             complement(<1..452)
                     /locus_tag="VN039_02830"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWV44954.1"
                     /translation="MPTHLIVDGYNLLAGTGSLSGHLESAREALVQDLAAYRHRKNHL
                     IIVVFDGWQQGRPSEQREHRAGVQVIYSKRGERADQVIQRLAREYGSDCAVVSSDHEI
                     VNAAKAHGAFIMGAREFAGKLRVSSGAVGTIPYKELDSGDDIRPRRGP"
     gene            579..1862
                     /locus_tag="VN039_02835"
     CDS             579..1862
                     /locus_tag="VN039_02835"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWV44955.1"
                     /translation="MQVRKPRLLSIKPLALLSCSVIFLLSAYGILIQAAWAWSPDDRH
                     PLPVMSSKHNRARGTTASLAPVTVRNFRVMTSPERTRLVLDLDRHTTVIEQRASNPSR
                     VVIALPNAWLGRPARIKARNGTIPSPFMITQLPHQAVAVSLSTTSFRTYKHFTLSHPP
                     RLVIDVTPSDAQVRPDAPDSHELPQRSISPVSQPVIPRAKGYTTIVIDPGHGGKDPGA
                     RGIRKTEEKDITLKVGLQLRELLRKQSGLRVLMTRDRDVFIELEDRAKFANSNEADLF
                     VSIHVNSHPSRAVKGIEIYHFGEAKDQRALEVAARENGTPISNTGVGWEYLVADLLAT
                     KKIEASLDLAWNAKEAMVSRMNGQYVLNDHGVKTAPFYVLRFTSMPSILAEIAYISNP
                     DEEDLLRKPVFVQDVAQSLYHGIVSFLANNRSDVR"
     gene            1893..2630
                     /gene="truA"
                     /locus_tag="VN039_02840"
     CDS             1893..2630
                     /gene="truA"
                     /locus_tag="VN039_02840"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWV44956.1"
                     /translation="MPIVKLILEYDGTAYAGWQRQPDQPTIQEAVETAILGVTQITAS
                     VIGAGRTDAGVHALGQVASFRIERDMTSREWTRALNAHLPKSIVVRSTTLMPDIFHAR
                     HSAKGKLYEYRILNRSERPAVARDYCWHIHQPLDDAAMNQAGLALIGPHDFSSFQTQP
                     TDNDDPMCHLQRFTVFREGDRLRIDAYADRFLKQMIRSIVGTLVEIGLGKRTPESIKP
                     ILQAHDRSAAGKTAPPQGLFLVRVDYD"
     gene            2726..3046
                     /locus_tag="VN039_02845"
     CDS             2726..3046
                     /locus_tag="VN039_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HWV44957.1"
                     /translation="MVISLRALTLGLFVLSLGVTPFAVAAPAQQNKMKACNEQANAKE
                     FGEGKGEERKAFMKECLSAKSEKSGSGKDTQQNKMKTCNKEAGEKKLKGDERKQFMSD
                     CLSN"
     gene            complement(3101..>3661)
                     /gene="epsC"
                     /locus_tag="VN039_02850"
     CDS             complement(3101..>3661)
                     /gene="epsC"
                     /locus_tag="VN039_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HWV44958.1"
                     /translation="GFHALLAYRITHWLKARDIPILPRVISQLARWVTGVEIHPSAKI
                     GTGFFIDHGMGVVIGETAEIGDYVTLFQGVTLGGTGKERGKRHPTLGNHVVVGAGAKI
                     LGGITIGDNVKIGANSVVLKNVAANSTVIGVPARVIKTQGTRLPDATMDQVDLPDPIS
                     DRLIALEQELIELRKRLENPDEQSHS"
BASE COUNT          830 a         1111 c          935 g          785 t
ORIGIN      
        1 tccggtccgc gccttgggcg aatatcgtcc ccagaatcca gttctttata tggtatggtg
       61 ccgaccgcac ccgatgacac acggagctta cccgcaaact cacgagcacc catgataaag
      121 gccccatggg ctttagccgc attcacgatc tcatgatcgg aactgacgac cgcacagtcc
      181 gagccatact cccgtgccaa ccgttggatg acttgatccg cccgttcacc ccgttttgaa
      241 tagatcacct ggactccggc acgatgctcc cgttgttccg atggccggcc ctgctgccag
      301 ccgtcaaata ccacgatgat gaggtgattc tttcgatgtc gataggctgc tagatcttgc
      361 accaatgctt cgcgggccga ttcaagatgc ccggaaaggc tgccggtacc agccagcaag
      421 ttatacccat cgacgatcaa atgcgtcggc atacctcacg ctatcgtggt catgagcaga
      481 cagtcaatcc gaactccgca tagccaactg gtgtaattct tgacaaggcg gcggacatct
      541 gagaatagtt tctcattgtc ctctacccca aagggcccat gcaagtacga aagcctcggc
      601 tgctttcaat caagcccctc gctcttctct cgtgcagtgt cattttcctc ctgtccgcat
      661 acgggatcct gatccaagcg gcctgggctt ggtcccccga tgatcgccat cctctgccgg
      721 tgatgtcatc gaaacacaac cgtgccagag gaacgactgc ctcccttgct cccgttaccg
      781 ttcgtaattt ccgcgtgatg acgagcccag agagaaccag actggtttta gatctcgatc
      841 gtcacaccac tgtcatcgag cagcgagctt ccaatccaag ccgagtagtg atcgcgcttc
      901 ccaacgcatg gctcggccgg ccggcacgaa ttaaagccag aaatgggacc atcccctcac
      961 ccttcatgat cactcaattg ccccaccagg ctgttgctgt gtctctttcg accacatcgt
     1021 tccgaaccta taagcacttc acgctttccc atccgcctcg cttggtcatt gacgtgactc
     1081 catccgatgc acaagtccgc cctgacgcac ctgactcgca cgaactccct cagcggtcaa
     1141 tttctcctgt ctcgcaaccc gtgatccccc gcgccaaggg atacacgacc atcgtcatcg
     1201 accccggcca tggggggaaa gacccgggcg cacgggggat ccgaaagacc gaggagaagg
     1261 atattaccct caaggtagga ctccagcttc gcgagctcct gaggaagcag tccggcctgc
     1321 gggtcttgat gacccgggac cgtgatgtgt tcattgagct ggaagatcgc gccaaattcg
     1381 cgaacagcaa cgaagccgat ctctttgtct ccatccatgt caactctcat ccatcgcgtg
     1441 ctgtcaaagg gattgaaatc tatcacttcg gagaggctaa ggatcagcgg gctctcgagg
     1501 tcgcagcacg ggagaacggc acccccatca gcaacaccgg agtcggatgg gaatacctcg
     1561 tggcggatct cttagcgacg aagaaaatcg aggcttccct tgatctcgct tggaacgcga
     1621 aagaagccat ggtttcccgg atgaacgggc aatacgtact caacgaccat ggcgtcaaga
     1681 cggcgccctt ttatgtattg aggtttacga gtatgcccag cattctggcc gagatcgcct
     1741 atatttccaa ccccgacgaa gaagatctcc tcaggaaacc ggtgttcgtt caggatgtcg
     1801 ctcaatccct ctaccatggt attgtttcgt ttcttgccaa caatcgatcg gacgtccgat
     1861 gatcgttttg ctcatcacca tccacgtgct tcatgcccat cgtcaaactc attctcgaat
     1921 atgacggcac ggcctacgca ggctggcaac gtcagcccga ccaacccacg attcaggaag
     1981 ctgtcgaaac agctatccta ggcgtcactc aaatcaccgc atcggtcatc ggcgccggac
     2041 gcactgatgc gggtgttcac gcattggggc aggtcgcgag cttccgtatc gagcgtgaca
     2101 tgacgtcccg agaatggacc agggcgctga acgcccatct cccgaaaagc atcgtagtgc
     2161 gatcaaccac actcatgccg gacatattcc acgcgagaca ttcggccaag ggtaaactgt
     2221 atgaataccg cattctgaat cgctcggagc gtccggcggt tgcgcgtgac tactgctggc
     2281 atatccacca gcctcttgac gacgcggcga tgaatcaagc aggacttgcc ctgatcggtc
     2341 cgcacgattt ctcttccttc cagacccagc ccaccgacaa cgacgatcct atgtgtcatc
     2401 tacagcgatt cacagtcttc cgtgaaggtg atcggctgcg gatcgacgcc tatgccgacc
     2461 ggttcctgaa acagatgatt cgctcgatcg tcgggacgct cgtggagatc ggcttgggca
     2521 aacgcacgcc ggagagcatt aagcccatcc tccaggcgca tgaccgttcg gccgccggta
     2581 aaacggctcc cccacaagga ctctttttgg tacgggtcga ctacgactga cccctgcccc
     2641 attcattgac agacaattac gtatcgcttt gctatgatgc ggtccagact tacagctcat
     2701 ccctcactac agaaggagat ccagcatggt gatatccttg cgtgccctca cactcgggtt
     2761 gtttgtgctc agtcttggag tgaccccgtt cgccgtggcc gcacctgccc agcaaaataa
     2821 gatgaaagct tgtaatgaac aggcgaatgc gaaggagttt ggcgagggga aaggggagga
     2881 acggaaggca tttatgaagg aatgtctctc cgccaagtcg gagaaaagcg gaagcggcaa
     2941 ggacacgcag cagaataaga tgaagacatg caataaagaa gcgggcgaga agaagttgaa
     3001 gggcgacgag cggaaacaat tcatgagcga ttgcctttca aattagccga tggccgcgcc
     3061 agcctgattt cgtgcacgtg cctgcctgta tcgatgactg ttacgaatgg gactgctcat
     3121 cgggattttc gagtcttttg cggagttcga ttaattcttg ttcaagggcg atcagccgat
     3181 cactgatcgg gtcgggcaga tcgacctgat ccatggtcgc atccggtaaa cgcgtcccct
     3241 gagtcttgat cacccgagca ggcactccta tgacagtgga attggccgcg acgtttttga
     3301 gcaccacgga attcgccccg atcttcacgt tgtccccgat tgtaatccct cctaagatct
     3361 tggcacctgc gcccaccacc acatgattac cgagtgtcgg atgccgtttg ccccgctcct
     3421 tgccggtgcc acccaaggtc accccttgaa agagggtcac gtaatccccg atttccgccg
     3481 tttctccgat gacaactccc atcccgtggt cgatgaaaaa gccggtcccg atttttgcgg
     3541 aaggatggat ctccacaccg gtcacccagc gcgccaactg cgaaatgacc ctcggcagga
     3601 tcgggatatc gcgcgccttc agccagtgag tgatgcgata cgccagaagc gcatggaacc
     3661 c
//
LOCUS       DATMTI010000172         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_558497, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000172 DATMTI010000000
VERSION     DATMTI010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558497"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..292)
                     /locus_tag="VN039_02855"
     CDS             complement(74..292)
                     /locus_tag="VN039_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44959.1"
                     /translation="MIRILCALPFALLAGAMVAGCADDSFSGRQRPAPGYDNVNMGSS
                     QPGQQHRYDEPPPASQRDPYSRSQPGYY"
BASE COUNT          394 a          481 c          428 g          343 t
ORIGIN      
        1 tcaataccgt gttgaggctc tcaccgacgc tgggggcgca gcacatcttc tgaatccgtg
       61 gcagggcatc ctgctaatag tatccaggct ggctacggct ataaggatca cgttgtgaag
      121 ccggcggagg ttcgtcgtaa cgatgctgtt ggcccggctg cgacgatccc atattgacgt
      181 tatcatagcc aggcgcgggt cgttgtcgac ccgaaaaaga atcatcggca cagccggcga
      241 ccatggcacc cgccagaagg gcaaagggca gagcgcatag aatacgaatc atacgaaacc
      301 tccttcgtca ttcggaatta tttcgatgca tttctcatcc attcagtctg ctgtacgtac
      361 aaggtaccgc taccttacaa gccatcttca acctgcgaag agtcacgtga tcatttgaac
      421 tcagccaact gggcacatta attggtgtgc cccccatttg ttgccacgca tcgtcttctc
      481 aattttgcca tgtatcccat ccacggcgca aaggtagatg tgcgacccct ctgttttcgg
      541 gaagaataaa tgggacgaga gttggcattc atcgcttctc acaaggactt cgccgtaagc
      601 atacagcacc gtcacctttc aaacgtggca cggagttgca gcacgacgcg gcgctggcag
      661 tgcctcgccc cagtgatgct gtccgtcact ctgctcctcg atcatcatcg aagggccgag
      721 cccagcaaga acggactcaa acgtcggact cgcgcaccga cctcttttca acacattggt
      781 gcgcccctca cgacactggg gcgcaacaga atctccagtc tgtgaccacg cacgactgca
      841 agcgtgacat cgtctgcggt ggcgcggttg ccttgggcgt accacacccc ttgaggagcg
      901 acggatattg tctccgcacg gtcgagtcgc gtcgcgcgac acgacggtgt ccgactccac
      961 gtggcacggc gtctccggac gcggtgctga agcccgtcgc ccagcgcagg tcagcaacct
     1021 ctcagtagaa actcgcccga cactcaggtt gctgcggacc caggtgagtg actcctcggg
     1081 acttggacgt acccgacacc acaatccctt cttttgtgca ccccctactt ttcggtatga
     1141 agaggtttta tcaccctgcg gcgtcaggac accaagacaa agaagccgag acaagtcccc
     1201 atgacgccag atgtgaaagc cacgttgcag cggttagcca aaatacggag tctctcaacg
     1261 agacacgtgt ttttgtacaa ccggcaacca ttgagggatt ttcgcacggc atttagaaca
     1321 gccctgacag aggcaggctt aagtgacttt cgcttccatg accttcggca ttgtgctgct
     1381 acaaacctac ggagagctgg aattgacaca accacagcca tgcagattgt aggccataca
     1441 tccccgcaga tgtggaaacg atacaatcag atcagagagg gggacctcac acaggccgca
     1501 agtaagcttg ggaaatatct ccaagagaac acgcccggaa cacttgaccc caaggccgag
     1561 agtctgtaac actgtaactc attaaaatca ttgggatgct ggcgtagctc aatcggcaga
     1621 gcagcggttt tgtaaaccgc aggttg
//
LOCUS       DATMTI010000173         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_49099, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000173 DATMTI010000000
VERSION     DATMTI010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49099"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..632
                     /locus_tag="VN039_02860"
     CDS             138..632
                     /locus_tag="VN039_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44960.1"
                     /translation="MRKYTAEQVRNVARLLDGESRSMLNEYAALLRERESAKAAVTDE
                     MVECACDGFMRVRGIGMHLAWQSMTEETRISYRNAMRPALETVAPMLASARVPDGWRL
                     GDVDVAQGGDMWTAGIDVPVDRRYSGDGIHASAIECHAATKQLAEALRAQVMLAAAPK
                     PEKE"
     gene            671..1126
                     /locus_tag="VN039_02865"
     CDS             671..1126
                     /locus_tag="VN039_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44961.1"
                     /translation="MDTCTRGIDGCICLREWKENAANILRTIAYPKHGTDEESTTILD
                     AAQIIQATFSADDLSTETRIITIVDGLQTKVEFTTEPPIEIGWYFVNNGKDPADEHWS
                     IKMIFGYNDSHGLGMRVPNCGKEEWYPLSDSRFDGWRYMGPITPAAFRA"
     gene            1180..1407
                     /locus_tag="VN039_02870"
     CDS             1180..1407
                     /locus_tag="VN039_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44962.1"
                     /translation="MRKHINVTRQQLNNIVAANPTFLIEWDTFGIPDLLTSKELDLKT
                     RRIENARGPKMFDVEVNEVFRTVHVTQVDTV"
     gene            1404..2060
                     /locus_tag="VN039_02875"
     CDS             1404..2060
                     /locus_tag="VN039_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44963.1"
                     /translation="MSVQAPWEKDTSPEDKNILERLLNGTASIEEIFGPSAIAPAVEP
                     AAPRAVAPVTTPPAGPIVPPLLPHVQHNVGPGSRYADLFDQAVLDDAKSRVAQYDHEK
                     IGRYDPFEPSRLADQLEREAHSQLLNCRVTTFVLACSHAVLLTEVWKAEDESIVELGG
                     TIEDQRAKKREEADRKAQAVEVARQKWKEAILEAKEGKKKWDDYVAEMKRRFQIARDG
                     "
BASE COUNT          552 a          560 c          640 g          437 t
ORIGIN      
        1 gcggcagagc atgcgctgcg tgtatttgat ggtctgccgg acaattggac accctctccg
       61 tctgcggtga tgcacatgca cgaagcattg attgcggttc tacccgcgct tacgcgccct
      121 tcgaaggatt aattgatatg cgcaagtaca cagcggagca ggttcgcaat gttgcgcgtc
      181 tactggacgg cgagagcaga tcaatgctta acgaatacgc cgccctcctg cgcgagcgcg
      241 aatcggcgaa ggcggcggtg acggatgaaa tggtggaatg tgcgtgcgac gggttcatgc
      301 gggtgcgcgg aatcggtatg catttagcat ggcaatctat gacggaggaa actcgcatta
      361 gctatcgcaa tgcaatgcgc cccgccctcg aaaccgtcgc gccgatgctg gcgagtgcgc
      421 gggtgccaga tgggtggcgt cttggtgacg ttgacgtggc acaaggcggt gatatgtgga
      481 cagctggaat cgacgtcccg gttgaccgtc gatacagcgg agacggaatt catgccagcg
      541 ctattgagtg tcacgctgct acaaaacaac tagcggaagc gctgcgagcg caggtcatgc
      601 tcgccgcagc cccgaagccc gagaaggagt gagagcaatg gttgcttgcg caaaagtttt
      661 aggcaggacg atggatacct gcacccgagg gatagatggc tgcatctgtc tgcgtgaatg
      721 gaaagaaaat gcagccaaca tcctaaggac gattgcatat ccaaagcatg gaacggacga
      781 agagagcacg actattcttg acgcagcgca aatcattcaa gcaacgtttt cggcagatga
      841 cttatctacc gagacgcgaa taatcacgat cgtcgatgga ctgcaaacga aggtggaatt
      901 cacgaccgaa cctccgatag agattggctg gtatttcgtc aacaacggga aggatcccgc
      961 agacgagcac tggtcgatca aaatgatctt cggctacaac gacagccatg gacttgggat
     1021 gcgcgttcca aattgtggaa aagaagagtg gtatccgttg agtgattccc gattcgatgg
     1081 ttggcgttac atgggcccaa tcactcccgc tgcgtttcgc gcataaaatc gtttacaggc
     1141 gttaggcccg cttgtatact gcgcgtcata ggaggaccca tgcgcaaaca cataaacgtc
     1201 acccgtcagc agctcaacaa tatcgttgcg gcaaatccga catttcttat cgaatgggat
     1261 acattcggca tcccggatct cctcacaagc aaagagcttg accttaaaac gcgcaggata
     1321 gagaatgcgc gcggcccgaa gatgttcgat gtcgaggtca atgaggtctt tcgcaccgtt
     1381 catgtcactc aggtggatac agtatgagcg ttcaagcgcc ttgggaaaaa gatacgtctc
     1441 cggaagacaa gaatattctc gaacgtcttt tgaacggcac agcgtccatt gaagaaatct
     1501 tcggaccgag cgctatagcg ccggcggtag agcccgcagc gccgcgcgcg gttgcccccg
     1561 ttactacgcc accggcaggc cccatagtgc cgcccctgct gccccacgtt cagcacaacg
     1621 tagggcctgg gtcgcgctac gcggatctgt ttgatcaggc ggtgctcgac gacgccaaat
     1681 cgcgtgtggc acagtacgat cacgaaaaga tagggcggta cgatcccttc gagccttcgc
     1741 gtcttgcgga tcaactggaa cgtgaagcgc acagtcagct tctcaactgt cgagttacga
     1801 cgttcgttct cgcatgcagt catgcggtcc tgctcaccga agtctggaaa gcggaggatg
     1861 agtcgattgt agaattgggc gggacaatcg aggatcagcg cgcaaagaaa cgtgaagaag
     1921 ccgaccgcaa agcccaagct gtcgaagttg ctcgacaaaa atggaaggaa gccattctcg
     1981 aggccaagga aggaaaaaag aagtgggacg actacgtcgc cgaaatgaag cgacgctttc
     2041 aaattgcacg cgacggttaa gctggggttg tgatttgtaa ttaacgcgtg taaggtatgc
     2101 cgctaccgcg caacctgcgc agtgccgttc accgagtcgg aagaagatat ggatacgccg
     2161 cagaagcgat tcgacactca gtacatcac
//
LOCUS       DATMTI010000174         5533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_346314, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000174 DATMTI010000000
VERSION     DATMTI010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346314"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..609
                     /locus_tag="VN039_02880"
     CDS             37..609
                     /locus_tag="VN039_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HWV44964.1"
                     /translation="MSTTDQPKWAWTRRSFLQVSLVGTLLLSGRLAGPQPVQARELPE
                     GRLTLVNVWTNERLDVTYRDEAGNYDLAALDDVNYLLRCHYTGEVGAIDVRVLEHVNL
                     VQKKLGSLEEIHVISGFRSPEYNAMLVRTDRRVAKNSLHMQGQAIDLLIPGISTTKLR
                     QAALELRYGGVGFYKRSSYVHLDSGPFRHW"
     gene            complement(708..2930)
                     /gene="glgB"
                     /locus_tag="VN039_02885"
     CDS             complement(708..2930)
                     /gene="glgB"
                     /locus_tag="VN039_02885"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWV44965.1"
                     /translation="MSLELTIQRDDLDRLIAGTHWSPHSILGPHAITIDGRPFLAIRT
                     WLPRVKDVEVVSNPSLWRMTRVREEGLYEVLLPGETQALSYRLRITHHGGTVTELHDP
                     YAFPPLLTDFELHLFAEGTLYKAYDNFGAHVRTVEGVCGVHFVVWAPNAVRVSVVGDF
                     NGWNGLCHPLTSRGATGVWELFIPDLPEGTLYKYEIRSREHTAPLLKADPYAVASELR
                     PRTASVVHDLSTYTWHDGEWMAARAEWDPLTASVSIYEVHLGSWMRVPEENNRWLTYE
                     ELAAKLIPYATDLGYTHLELMSVTEHPFDGSWGYQSTGYFAATSRYGDPKGFMAFVDA
                     AHQAGLGVIMDWAPAHFPDDSHGLALFDGTHLYDHADPRLGYHPDWHSRIFNYDRVEV
                     RTFLLNSALFWLDKYHIDGLRVDAVASMLYLDYGRRSGEWIPNEFGGKENLGAVSLLK
                     ELNVLIHREFPGAVTIAEESTAWPGVSRPTYTGGLGFTFKWNMGWMHDMLAFFQHDPI
                     YRRFHQNQITFGLLYAFSENFILALSHDEVVHGKRTLLDKMPGDVWQRFSNLRLLYGH
                     MYSHPGKKMLFMGGEFGQWREWNHDGSLDWHLCEEEPHRGLQRLIRDLNRVYREEPAL
                     HEVDFDWSGFQWIDFSDADNSVIAYLRKAKSTQTAIVCLCNFTPVPRLGYRIGVPEPG
                     WYHELINTDGIAYGGGNMGNGGGVHTAELPSHGFRYSLTVTLPPLSILWLKQPPTNNL
                     "
     gene            3098..4438
                     /locus_tag="VN039_02890"
     CDS             3098..4438
                     /locus_tag="VN039_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HWV44966.1"
                     /translation="MSSLMLALLIFGLCYVLIMTERLHKTIVALFGAAVMIGLGVVSQ
                     EEAFYSHEFGVDYNVVFLLIGMMVIINIVRETGLFEVLAVWAAQRADAKPFRLLVLLA
                     ILTAVLSAMLDNVTTVLLMAPVTLAITKRLELNPVTFLMTEALASNIGGTATLVGDPP
                     NIMIASKAELSYLDFLVVMGPIVIVIMAVFLTALWFIFGRTMTVAPHLREAVLALSSR
                     ESVPDEAFLRRCLILLAVINVGFCVHSLVHLEPATIALLGASLFMVIGHARRKPEDAE
                     ELTYLAEVEWKTIFFFIGLFILVGGLVKVGVIRYLADQLVAVTRGNLTGSTMAVLWGS
                     TILSAVVDNIPYVAAMNPLIVDLARSLHPEITDYVALVHQPDILPLWWALALGACLGG
                     NGTIIGASANVVIVDIARKAGYRITFWQFLKFGFPVMIGSVALSAIYLWLVFLR"
     gene            complement(4445..>5533)
                     /locus_tag="VN039_02895"
     CDS             complement(4445..>5533)
                     /locus_tag="VN039_02895"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HWV44967.1"
                     /translation="GDGAELPKRATLIGNLTVGMTPNYGVSASVTSVTLNTIDPCKIS
                     IADTDVLQEVKNQLTETVRGGLGNAVARINTLTARSRVENVWHTLRNPIPLEPDAWLL
                     LNLGNVWRSGFFSGGGDLIDDTIHITAKPVLVFGAEPPSAGGELPQLETEPASTGYHG
                     AADAQLYGTLPRTLANRLASSGFHVVADIPLDYPSLSRSLATRLQGKQVAVKGDFIHI
                     TDAAIFGRGGNQVVLRIAFTGDAIGHLYFVGKPEMNMLAQSVQISGLRLDLDSEQLLQ
                     KSGPDWLAGSSIRELVMGEAVVGVTPAIDRMRDLVTKALNRELSPTITTHGTVTSVQG
                     IGVFADVNALYVRVMSDGLLSLKVDGKQ"
BASE COUNT         1139 a         1498 c         1612 g         1284 t
ORIGIN      
        1 aatgatcacc gttcactttt ttctggagtt gtttcagtga gtactacaga tcagcccaaa
       61 tgggcctgga ccaggcgatc gtttcttcag gtttccttgg tggggactct attgctgagt
      121 ggacggttag ctggtccaca acccgtgcag gctcgtgaac tgcccgaggg caggctgacg
      181 ctggtaaacg tgtggaccaa tgagcggttg gatgtcacct accgagacga agccgggaac
      241 tatgaccttg cggcgctcga tgatgtgaat tacctcctgc gctgccatta cacgggtgaa
      301 gttggggcga tcgacgtccg tgtgctggag cacgtgaatt tggtgcagaa gaagctcggc
      361 agtctggaag aaattcatgt gatttccggc tttcggtctc ccgagtacaa tgccatgtta
      421 gttcggacag accgacgagt cgccaagaac agtttacaca tgcaaggaca ggcgattgat
      481 cttctcatcc ccggtatttc tacgacaaag cttcgccaag ccgcgctcga actgcggtac
      541 ggcggggtcg gcttctacaa acgctcgagc tatgtgcatt tggattccgg tcccttccga
      601 cattggtagc cctcgctcct gctgcaggcg aatcttttcc ttcttccaac acatcagttt
      661 acgaaaatga tcgggactgt cgttgtgtcg aaaccgggta gtgggggtta taggttgttg
      721 gtcggcggct gtttcaacca gaggatggat aggggaggga gggtgaccgt caaagagtag
      781 cggaacccgt ggctcggcag ttccgcagta tgaacacccc cgccatttcc catattgccg
      841 ccaccgtaag cgatcccgtc ggtgttgatc agttcatgat accaacccgg ctctgggacg
      901 ccgatccggt aaccgagacg gggtacgggc gtgaagttac acaggcagac aatagccgtt
      961 tgcgtgcttt ttgcctttcg caggtaggca atgacggaat tgtcggcgtc gctgaagtcg
     1021 atccactgaa atccgctcca atcaaagtcg acctcgtgca gggcgggttc ttcgcgatag
     1081 acgcggttca agtcgcggat cagacgttgc aagccacgat gcggctcctc ctcgcagaga
     1141 tgccaatcca agctgccgtc atgattccat tcccgccact gaccgaactc tccgcccatg
     1201 aagagcattt tcttacccgg atggctgtac atatgtccat agagtagccg aaggtttgag
     1261 aaccgctgcc acacatcgcc gggcattttg tccagcaagg ttcgtttacc atggaccact
     1321 tcgtcatgtg agagggcgag gatgaaattc tcgctgaagg catagagcag accgaacgta
     1381 atttggtttt gatggaaccg acggtagatc ggatcgtgct ggaaaaaggc cagcatgtcg
     1441 tgcatccatc ccatgttcca tttgaacgta aagccgagcc cgcccgtata ggtcggtcga
     1501 gacacgccgg gccaggccgt ggattcttcc gcgatcgtga cggccccggg gaattctcgg
     1561 tggatgagca cgttgagttc tttcaggagt gagacggctc caagattttc cttgccgccg
     1621 aactcattgg gtatccattc accggacctt cgaccatagt cgaggtagag catcgatgct
     1681 acggcgtcta cccgcaatcc gtcgatatgg tacttgtcca accagaagag cgcgctgttc
     1741 aataggaagg tgcgtacctc gactcggtcg taattgaaga tccggctgtg ccaatcgggg
     1801 tgatagccga ggcgggggtc ggcatggtca tagaggtgtg tgccgtcgaa cagcgccaat
     1861 ccatgggaat cgtccggaaa atgcgcggga gcccagtcca tgatcacgcc gagaccggct
     1921 tggtgagcgg cgtccacgaa cgccatgaat cccttgggat ctccatatcg gctggtggcc
     1981 gcaaagtagc ctgtcgattg atacccccat gatccatcga acgggtgttc ggtcaccgac
     2041 atgagttcga ggtgtgtgta gcccagatcc gttgcgtagg ggatcagctt cgccgcgagt
     2101 tcttcatacg tgagccaacg attgttctct tccggtaccc gcatccatga cccgagatga
     2161 acttcataga tggagacgga agcggtcagc ggatcccatt ctgctcgggc cgccatccac
     2221 tcgccgtcat gccaggtgta ggtggataag tcatgcacga ccgacgcagt tcgtgggcga
     2281 agctcgctcg cgacggcgta gggatccgct ttcagcaacg gggcggtatg ttctcgtgat
     2341 cggatttcgt atttgtagag ggtaccttcc ggtagatcgg gaatgaagag ttcccagacc
     2401 ccggtagctc cacgactcgt caagggatgg cacagaccat tccatccatt gaaatcaccg
     2461 acgacgctta cgcggacagc attgggagcc cacaccacaa aatgaactcc gcagacgcct
     2521 tcgacagtgc ggacatgggc gccgaaattg tcgtaggcct tgtacagggt tccttcggca
     2581 aagagatgca gttcaaaatc cgtcaacagc ggggggaaag catagggatc atgaagttcc
     2641 gtgacagtgc cgccgtggtg cgtgattcgc agccgatatg acagcgcttg ggtttcacct
     2701 ggaagaagca cctcatacag tccttcctca cgaacacgag tcatacgcca gaggctgggg
     2761 ttggatacaa cctctacatc cttgacccga ggcagccagg tccggatcgc gaggaacgga
     2821 cggccgtcga ttgttatggc atgtgggccc agaatcgaat ggggagacca gtgtgtgcct
     2881 gcaatgagac gatcgagatc atcccgttga atggtcagtt caagagacat cacgctatcc
     2941 taatggttac ccgcttgatt aggaaggaga aaatgttctc ccgtgacggc ttcggatgac
     3001 agaccagaaa gacgactctg attgcattgt cgtgactggc cccggataat gcgatggctc
     3061 tcaggacgat ccgtctccgt tcggttcaag gacatacatg tcatctctga tgctggccct
     3121 tcttatcttc ggcctgtgct atgtgctcat catgaccgag cggctccaca agaccattgt
     3181 ggccttgttc ggagcggccg tgatgatcgg gctcggtgtg gtctcgcaag aggaggcgtt
     3241 ttattcccac gagttcgggg tggactacaa cgtcgtcttc ctgctgatcg gcatgatggt
     3301 gatcatcaac atcgtgcggg agaccggact gtttgaggtg ttggccgttt gggcggcaca
     3361 gcgagccgat gcaaagccct tccgattact cgtgctgtta gccattttga cagcggtctt
     3421 gtccgccatg cttgataacg tcaccaccgt tctgctcatg gccccggtca ctcttgcgat
     3481 caccaagcgg ttagagttga accctgtcac gtttctgatg accgaggcgc tcgcctccaa
     3541 tatcggcggg accgctacgc tcgttgggga tccgcccaac atcatgattg ccagtaaggc
     3601 cgagctcagc tatctcgatt tcctggtcgt catggggccg atcgtcatcg tcatcatggc
     3661 ggtattttta acggccttgt ggttcatctt tgggcgaacg atgacggtgg ccccgcatct
     3721 tcgggaggcg gtgcttgctc tgagctcacg ggagtctgtt ccggatgagg cctttttgcg
     3781 ccgctgcctg attttgctgg cggtgatcaa cgtgggattt tgcgtccatt ctctcgtcca
     3841 tttggaaccg gccacgattg cgttgctggg agcaagccta ttcatggtga tcggccatgc
     3901 ccgacgaaaa ccggaggatg cggaggaact gacctatctg gcagaagtgg agtggaagac
     3961 catattcttt ttcatcggat tatttatcct ggtgggagga ttggtcaaag tcggcgtgat
     4021 ccgctatctg gctgatcaat tggtggcggt gaccaggggc aatctcaccg gctccacgat
     4081 ggccgtgctg tggggatcga cgatcctttc tgccgttgtt gataatattc cctatgtcgc
     4141 ggcgatgaat ccattgatcg tcgatcttgc ccggtcgctc catccggaga tcaccgacta
     4201 cgtcgccttg gtccatcaac cggacatcct cccgctctgg tgggcactgg ccttgggcgc
     4261 ttgtttaggc ggtaacggta cgatcatcgg cgcgagcgcc aacgtggtga tcgtggacat
     4321 cgcgcgcaaa gccggctacc gcattacctt ttggcagttc ttgaagttcg gatttcccgt
     4381 catgatcggg tcggtggcgc tcagcgcgat ctatctctgg ctggtattcc tgcgctaagt
     4441 cgaattactg cttaccgtcg accttcagac tgagcaaccc gtcactcatc acccggacgt
     4501 acagcgcgtt cacatcggcg aacacgccga tgccctgcac cgatgtgacg gtcccgtgcg
     4561 tcgtgatggt cggactcaac tcccgattca gggccttcgt cacaaggtcg cgcatgcgat
     4621 caattgccgg agtcacaccg accacggctt cacccataac gagttctctg atggaagaac
     4681 cggcgagcca atcggggccg ctcttctgga ggagctgttc ggaatcgagg tccaagcgga
     4741 ggccactgat ctgaacggac tgggccaaca tattcatttc aggtttcccc acgaaataca
     4801 ggtggccgat ggcgtctccc gtaaacgcaa tccgtagaac aacttgattg ccaccacggc
     4861 caaaaatggc cgcatcggtg atgtgaatga aatctccttt cacggcgacc tgtttcccct
     4921 gcaaccgcgt tgcaagtgat cgggacagcg agggatagtc cagtgggatg tcggcgacga
     4981 cgtggaaccc actggaagcc agtcggttcg ccagtgtcct gggcagtgtg ccgtacaact
     5041 gggcatcggc ggcgccatgg taccccgtgg aagccggttc agtttcaagt tgaggaagtt
     5101 ccccgccggc agatggtggt tcggcaccga aaacgagaac cggtttcgcg gttatatgaa
     5161 tggtgtcatc gatgaggtct ccaccccctg aaaagaagcc actgcgccac acgttaccga
     5221 ggttgagcag gagccacgcg tccggttcta gtggtatggg gttgcgcagc gtgtgccaga
     5281 cattttccac tcgggatctg gctgtcagcg tgttgatgcg tgcgaccgcg ttgccgagcc
     5341 ccccccgtac agtctctgtg agctgatttt tgacttcctg aagcacatca gtatcggcaa
     5401 tgctgatttt gcaaggatca atggtgttca gcgtcacact ggttactgat gccgacacac
     5461 cgtaattcgg tgtcatgccg acggtgagat tcccaatcag cgttgctcgt tttggcaatt
     5521 cggcgccatc gcc
//
LOCUS       DATMTI010000175         1691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_643792, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000175 DATMTI010000000
VERSION     DATMTI010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643792"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..466)
                     /locus_tag="VN039_02900"
     CDS             complement(170..466)
                     /locus_tag="VN039_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44968.1"
                     /translation="MEDWALIRRLVADGVDPLGLNTGNYAVDCATNALIGAGIGLLGD
                     AETAGASSLVGAIAGCGLGLLSDYLEENGYSSYRSDIETGAGLESLIEIALLFS"
     gene            complement(672..932)
                     /locus_tag="VN039_02905"
     CDS             complement(672..932)
                     /locus_tag="VN039_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44969.1"
                     /translation="MSTSNVAIRAWPWITAVIGLAIIVAEYIDGAINATWFDDHGIPS
                     PTPADLGFLIGALGFVLIVGGLIIGAGDYAKRRRSRRAQRSD"
     gene            complement(949..1251)
                     /locus_tag="VN039_02910"
     CDS             complement(949..1251)
                     /locus_tag="VN039_02910"
                     /inference="COORDINATES: protein motif:HMM:NF022240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="HWV44970.1"
                     /translation="MRKTIGKTVRWSYAYSGDPRLMVYPTVWGRWWSGSGMWGIQWKE
                     VVSKTTSYRSRANTADMKYQFECHLGLGANLWKESYNLDTELHRGSLLAYYRYKCN"
     gene            1280..1507
                     /locus_tag="VN039_02915"
     CDS             1280..1507
                     /locus_tag="VN039_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44971.1"
                     /translation="MKLRRRPVVQALGLWTMVAVFGLGFAIVGVQLHGLTTTAAVVTW
                     LLAGAAIGGLATVLIAPVAGFEVEEEDSTSR"
BASE COUNT          325 a          504 c          472 g          390 t
ORIGIN      
        1 atgcccacga ctagcggcaa aaccccgagg tgttgtccct tcggcggcag ggtaaacgcg
       61 actactattg ccaaggttcc caagactaag catgacgcag tgttggccac gagtatcagc
      121 cggttccttc gagcaaatcc ccttggtgac attgtgaacc cttcaggcct tatgagaaca
      181 gcaacgcaat ttcgattagc gattcgagtc ccgcacctgt ttcaatgtcg ctcctatagc
      241 tggaatagcc attctcttct agataatcac tgaggagacc gagcccgcat ccggcaatgg
      301 ctccgacaag cgatgacgcc ccggcggttt ccgcgtcgcc cagcagccca attcccgcac
      361 ctatcagggc attggttgcg cagtccacgg cgtagttgcc tgtgttcagc cctaatgggt
      421 cgacaccatc cgcgaccagc cgccggatca acgcccaatc ctccatcgag atcacccatc
      481 caatctgtct gggtggcctc gttttcgccc gtcgctaatg cctcaaattc gaccgtcgcc
      541 gacagagtat tcgctcgcca tcatcggccg gctcgtcatc gctgcctcga gaagcatctg
      601 agacagagcg gtgcgcgtgt gtgccgcgtg tagctcgttc tgtcagagag tgctggccgc
      661 ctcgacgggg gctaatccga cctttgggct cttcgtgatc gtcttcgctt ggcgtagtcg
      721 cccgctccaa taattagtcc tcccacgatg aggacgaacc cgagagcgcc gattagaaag
      781 ccaaggtcgg ctggtgtggg gctggggatc ccatggtcgt cgaaccatgt ggcgttaatc
      841 gctccatcga tgtattcagc gacaatgatt gcaagaccga taacagcagt gatccagggc
      901 catgcccgga ttgcgacgtt cgaggtgctc atgtgtccca tcctccggct agttgcattt
      961 gtagcggtag taagcgagca acgaccctct atggagctcg gtgtccagat tgtaggattc
     1021 cttccacaga ttggctccca gccctaggtg gcattcgaac tggtacttca tatcagcggt
     1081 gttcgctcgg gaccgatagg aggtcgtctt tgatacgact tccttccact ggatccccca
     1141 catccccgag ccggaccacc accggcccca taccgttggg tagaccatta ggcgtggatc
     1201 gcccgaatac gcatacgacc agcgcaccgt ttttcctatc gtcttcctca ccgtcaccat
     1261 ccaaaggcgc gacctaaaca tgaagctgcg gaggcgcccg gtcgttcagg cgcttgggtt
     1321 gtggacgatg gttgctgtgt tcgggctcgg cttcgctatc gttggcgtcc aactgcacgg
     1381 tctcacgacg acggcggcag tcgtaacgtg gctactagcg ggcgccgcga ttgggggatt
     1441 ggcgacagtc ttgatcgcgc ccgtagccgg tttcgaagtc gaagaggagg actctacgag
     1501 ccgctgacac ccttgccggc agtaggctcg acttcacgct tgtaggttgg cgcgctcact
     1561 accttacata tttgggtact cgatacgtcc ggattgcgtt gatgacatga aacgattgtg
     1621 ggttgttatc ccgcttggcg ctctcgtcgg agtggctttg gtgttggcaa gcatttacgg
     1681 cttctatcag c
//
LOCUS       DATMTI010000176         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_49220, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000176 DATMTI010000000
VERSION     DATMTI010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49220"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VN039_02920"
     CDS             <1..831
                     /locus_tag="VN039_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWV44972.1"
                     /translation="TPQRMTSSHAVLRLVRSVHEIPDHVSPQMRSGSKRGSVSTHEVP
                     LAIGPYGTGILYLERDAKHPFTEQERQLLAIVGASLALTLRNADSYQRLQDVALQDSL
                     TGVLNRRGLNTPLARELKAGLRYGVPACLILLDFDFFQTVNDCLGHKIGDHVLRAAAD
                     LIRMTVREIDIVGRHRGNTFAVVLPHTDRQQARALAERLRDRIERYPFAIEAGQVRTT
                     ASIGLTAVPDATVASIAEWMMICEAALNDAKAQGRNRVVLHAPKPPALACAVALSCAA
                     "
     gene            851..1456
                     /locus_tag="VN039_02925"
     CDS             851..1456
                     /locus_tag="VN039_02925"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV44973.1"
                     /translation="MASPVTTPSVPFKNSADERREWIRIDDRVLMEYRLLTDTGTVDP
                     VEADRTTPEMISAAVTKPSADLLARTGESLIASPVLPWIMKVDYLLQVILNSLVTIAP
                     SSVVMARLMDVNLSGGGVGFVSSHEFAVGDRLSVKMILPPFTPIHAVAQVIRSTPDSQ
                     RQGWVLATEFLEISSNDLDHLIRHILHAQAERLRARRNHPG"
     gene            1542..2552
                     /locus_tag="VN039_02930"
     CDS             1542..2552
                     /locus_tag="VN039_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWV44974.1"
                     /translation="MNQLNIKEIIGNIEKRIDDHRLVNARITSEINILSLNATIEAAR
                     AGDAGKGFAVVATEVKHLAAQAADASKALSSIREETSELNRRFMDKEHDRLSEMAQTL
                     VQLIVRNLYERTADVRWWAMDEALVKGLESLDRAALQHAVERLGLINRYYSVYLNLVL
                     VGIDGKIMACSHPEKFPRIIGADTTRVSWFQKAMATTNGDQYVVDDICHDPLHGDKLV
                     AVYAAAVRKGGNTNGRIIGVLGVVFDWEEQSRTIVQKEPAISEASWRRTRVMLLDNRL
                     RVIAASDHQGLLVPFTLQHQGRQRGHYVNDKDEVIAYAKSLGYQEYDGLGWYAVIVQK
                     LR"
     gene            complement(2676..3575)
                     /locus_tag="VN039_02935"
     CDS             complement(2676..3575)
                     /locus_tag="VN039_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWV44975.1"
                     /translation="MARVISVASGKGGVGKSVVSVNLALVLARKEKQVVLADLDVGGA
                     DAHVLVGLLNPPLTLTDFLNHRIERLDDLAQGLSIHPNLRIIPGTGDTLATANMAYSQ
                     KKRLIRNFQDIRADVIIVDIGAGTSYHALDFFLMADHHLAVATPDPTSVLDLYRFIKL
                     AAIRRVLSMFVMRDVMAEKLANRDYSSVEEVLDVAGKTDESGRSIAESTLKAFQPALI
                     LNRVSGRARVNVPQLKKLLKEYVGGDLTLLGEIPEDPAMEQAVRAYVPVVQHTPASPA
                     AVALAKTADALARLLHDAPPQAA"
     gene            3644..4021
                     /locus_tag="VN039_02940"
     CDS             3644..4021
                     /locus_tag="VN039_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV44976.1"
                     /translation="MDSIPSGMEEIRPPSQIRSYWTLKFLNRHADTSVVMNDEVSTAT
                     AIQAVEATAREGEILTVMEVARFLRVPKSTVYKLARVGELPASKIGKHWRFLRRDIHE
                     WMHGRSRQSERSASRPLFGSSAE"
     gene            4319..>4448
                     /locus_tag="VN039_02945"
     CDS             4319..>4448
                     /locus_tag="VN039_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV44977.1"
                     /translation="MKILVVDDMVTMRRIVKNILKQLGFVNVEEAENGQEALQKLQA"
BASE COUNT         1042 a         1173 c         1287 g          946 t
ORIGIN      
        1 acgccgcaac gtatgacttc gtctcacgca gtgctgcggc tcgttcgttc ggtacacgaa
       61 atccccgatc atgtatcacc tcagatgcgg agcggttcga aacgtgggtc cgtcagcaca
      121 catgaagttc cgctcgcgat cggaccgtat gggaccggca ttttatatct tgaacgagac
      181 gctaagcatc cctttactga acaagaaaga cagctcctcg ctatcgtcgg ggcatcactt
      241 gccttgacct tgcgcaatgc cgattcgtac cagcgtctgc aagatgtggc cttacaggat
      301 tcattgacgg gcgtcctaaa ccgtcgtgga ctcaacacgc cgcttgcccg agaactgaaa
      361 gctggattgc ggtatggcgt accggcctgc ctgatacttt tggattttga tttttttcag
      421 accgtaaacg actgtctcgg ccacaagatc ggggatcatg tgctcagagc ggcggccgat
      481 ctgatacgga tgacggtgcg tgagatcgat atcgtcggac gccacagagg taatacattc
      541 gcggttgtgc tgccccatac agatcggcag caggctcgtg cccttgcgga gcggttgcga
      601 gatcgcatcg aacgatatcc ctttgcgatc gaggccgggc aggtccgtac cacggcaagc
      661 atcggcttga ctgcggttcc agatgccacc gtggcttcga ttgcggagtg gatgatgatc
      721 tgcgaggccg ccctgaatga tgcgaaggcc caaggacgga accgcgtggt ccttcatgct
      781 ccgaaaccgc cggcattggc gtgcgctgtg gccctgagtt gcgccgcata acgtcgaaag
      841 tagcctgacc atggcgtctc cagtcacgac accttccgta ccattcaaga attccgctga
      901 cgaacggcga gaatggatca gaatcgacga tcgggtgctg atggaatatc gtttgctgac
      961 cgatacagga accgtcgatc ctgtcgaggc ggaccgcacc actccggaga tgatttccgc
     1021 agcagtcacc aagccgtccg cggatctgct ggcgcgcaca ggagagtcgt tgatcgcctc
     1081 accggtactt ccatggatca tgaaagtcga ctacttactc caagtcattc tcaattccct
     1141 cgtgacgatc gccccgtcaa gcgttgtcat ggcgaggctg atggatgtca atttgagcgg
     1201 aggaggagtg ggtttcgtgt cctcccatga attcgccgtc ggcgatcgat tatcggttaa
     1261 gatgatcctg cctccgttta ctcccatcca cgccgttgcc caagttatcc gatcgacgcc
     1321 ggattctcag agacaaggct gggttcttgc cacggagttt ctcgagatca gttccaatga
     1381 tctggaccac ctcatccgcc acattcttca cgcacaggcc gaacgcttgc gcgcacgccg
     1441 caaccatccc ggttagggga aggaatggtt cacggccata cggatgtcac cgaagaattc
     1501 aggcgtcgcc gtcaaagttg cggaaaatcc agaggacatg gatgaaccag ctcaatatca
     1561 aagagatcat aggtaacatc gaaaagcgga tcgacgatca ccggcttgtg aatgcaagaa
     1621 taacgagcga gatcaacatt ctttcgttaa atgcgaccat agaagcggcg agagccggtg
     1681 atgccggaaa agggttcgcc gtggtcgcga cagaggtgaa acacctggct gcgcaagcgg
     1741 ccgacgcctc caaagccctg agttcgatac gcgaagaaac cagcgaattg aatcgacggt
     1801 ttatggacaa agagcatgat cgtctctcgg agatggcgca gaccctggtg cagttgatcg
     1861 tacgcaattt gtacgaacgt accgctgacg ttcgttggtg ggcgatggat gaagcgttgg
     1921 tcaagggttt ggagtcgtta gatcgagctg ctctccagca tgcagtcgaa cgactcggtc
     1981 tgatcaatcg atattattct gtgtatctca accttgtatt ggttgggatc gacggcaaga
     2041 tcatggcctg ctcacaccct gaaaagtttc ctaggataat cggagccgat acaacgagag
     2101 tgtcctggtt tcagaaagcc atggcgacga cgaacggcga ccagtatgta gtcgacgata
     2161 tttgtcatga tcccctccat ggcgataaat tggttgccgt gtatgccgca gccgttcgga
     2221 agggaggaaa cacaaacgga aggatcatcg gcgttttagg ggtggtgttc gattgggagg
     2281 agcaatccag aactattgtt cagaaagagc cggcaatatc cgaagcgtca tggagacgca
     2341 cccgcgtgat gctgctggat aacaggctgc gagttatcgc ggcatccgat catcaaggac
     2401 tccttgtgcc gttcacgctt cagcatcaag gacgccaacg aggccattac gtgaacgaca
     2461 aggacgaagt gatcgcgtat gcgaaaagtc tcggctacca ggaatacgac ggcctcggat
     2521 ggtatgcagt gatcgtacaa aagcttcgat gatcactgaa gtctatcgtg cacaagcgac
     2581 cggttcacgc gggttccaag aacggctctc ttatgagtaa cgagtggaga ttgaggagaa
     2641 acggcatggg ggaatattcg gaccgctcaa ccgaatcatg ccgcttgcgg cggtgcgtcg
     2701 tgaagcagcc gtgccagggc atcggcggtt ttcgcgagcg cgaccgcagc aggagacgct
     2761 ggagtatgct gaaccaccgg gacgtacgcg cgtaccgcct gttccatcgc ggggtcttcg
     2821 ggaatctcac ccaataaggt taggtcacct ccgacatact ccttgagtaa cttcttgagc
     2881 tgcggcacat tgacgcgagc ccgccctgaa acgcgattga ggatgagagc cggttgaaaa
     2941 gctttgaggg tagactcggc aatcgaccga ccggattcgt cggtcttgcc tgccacgtcc
     3001 aacacttctt ccacactgct gtagtcacgg ttcgcaagct tctccgccat gacatcccgc
     3061 atgacgaaca tcgacaagac acgacgaatc gccgccagct tgatgaatcg gtagagatcc
     3121 aggacggagg tcgggtcagg ggttgcgacg gcaaggtgat ggtcggccat cagaaaaaaa
     3181 tcgagtgcat gatagcttgt tcctgctccg atatcgacga tgatcacgtc cgcgcggata
     3241 tcctgaaaat ttcggatcaa gcgctttttc tgtgaatagg ccatgttggc cgtcgccaat
     3301 gtgtcgccgg tgccgggaat aatccgcaag ttcgggtgaa tggagagacc ttgagccaga
     3361 tcatccaggc gctcgattcg atggttgagg aagtcggtca gggtaagggg cgggttgagg
     3421 agtccgacca gtacatgcgc gtcggcgccg ccgacatcga ggtctgcgag caccacttgc
     3481 ttctccttac gtgccaagac gagagccagg ttgacggaaa ccacgctttt gccgaccccg
     3541 ccttttcctg acgcaactga gatcacacgt gccattgagt acagttccta ttgatttggg
     3601 cccattggca catcctttgt cggtcagtcg cggagaaacg tgaatggaca gcattccgag
     3661 tggaatggaa gaaataaggc cgccgtccca aattcgttcc tattggacgc tcaagttttt
     3721 gaaccgccac gccgatacct cagttgtcat gaatgatgaa gtatctacag caacagcgat
     3781 ccaggccgtg gaggccacag cacgggaagg ggaaattcta accgtgatgg aagtggctcg
     3841 gtttcttcgg gtaccgaagt ccaccgtcta taaactcgcc cgagttggtg aactgccggc
     3901 atcaaagatt ggaaaacact ggcgttttct tcgccgtgat atccatgaat ggatgcatgg
     3961 tcgatcccgg caaagcgagc gatctgcgag tcggccgctg tttggctcat cggcagaatg
     4021 actcgattgt cgctctgtca ggcggcaggt cgctcggtct tgagcggcat ggccctgcgg
     4081 acgatgaccc ccgaggacaa gctttttcca cgcacgtcga tcatttccca cccatgcggc
     4141 gaagtcgaca acgcatcgaa ccattccggc tcaggtcgct cggcgaatgt gccgatatcc
     4201 cacgaagcgc cgagaggccg tgggacggtc ggactttccc atcatggaca agacgtcggc
     4261 catcgtcgct gtgacaggag ggtaagaaaa aggaggcgct atgccagccg atccgaacat
     4321 gaaaatcctt gtggtcgatg acatggtgac gatgagaaga atcgtcaaga atatcctgaa
     4381 gcaattggga ttcgtgaacg tggaagaagc ggaaaacgga caggaagcac tgcagaagct
     4441 gcaggctg
//
LOCUS       DATMTI010000177         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_49326, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000177 DATMTI010000000
VERSION     DATMTI010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49326"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VN039_02950"
     CDS             <1..709
                     /locus_tag="VN039_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HWV44978.1"
                     /translation="KLPVMLKGPTGCGKTRFVQYMAYKLGRPLITVACHEDLTASDLV
                     GRYLLKGQDTVWMDGPLTAGVKHGAIVYLDEVVEARKDTTVIIHPLSDDRRVLPIEKK
                     GQIVEAADEFMLVISYNPGYQSVIKDLKQSTKQRFMAIEFDYPAADIEAMVVQREAGV
                     DPAIAGSLVKLGQKVRNLRNHGLEEGVSTRLLIYAGTLMKQGVPPGRACDVAIARPIT
                     DDSDMQRAILEFVKAIF"
     gene            690..1040
                     /locus_tag="VN039_02955"
     CDS             690..1040
                     /locus_tag="VN039_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWV44979.1"
                     /translation="MSKRSFESIDSLPAIARDSERGALLTVHVQPGSSRTECVGIHGD
                     AIKIRLAARPIDGAANDELIRFIADRCAVPRANVQIRAGAEARRKRLCVKEISAQVLL
                     ARLLPRNEQETERI"
     gene            1037..1468
                     /locus_tag="VN039_02960"
     CDS             1037..1468
                     /locus_tag="VN039_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HWV44980.1"
                     /translation="MKGLRLSSAVLAVVMLSACSSARPVLYPNTQLESVGKDGAERDI
                     EACRQLAESAGAAEGGGGKGGRVATNTAVGAGVGAASGAVGGAIFGGVGRGSMIGAAS
                     GAVWGLLTGLFHAASPSQPNQAYTNFVNRCLQEKGYEVTGW"
     gene            1667..2101
                     /locus_tag="VN039_02965"
     CDS             1667..2101
                     /locus_tag="VN039_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44981.1"
                     /translation="MVNRLRHCCIVCALLVSLTACAGPQPILRSNKQLHFYGKQMAQQ
                     EVQSCRKKVEETGLQPGVHQSANAATGAILGLTLGGAVGASAGVVGGLPGVAIGAAAG
                     SGLGLVVGLLGGTFKPLEPEQPYADAVAGCLKEKGYEVGGWE"
BASE COUNT          491 a          562 c          719 g          434 t
ORIGIN      
        1 gaaactgccg gttatgctga aggggccgac cggctgcggg aaaacccggt ttgttcaata
       61 catggcgtac aagctcggcc gaccgctgat cactgtcgcc tgtcacgaag atctcacggc
      121 atccgacttg gtaggccgtt acctcttgaa ggggcaggat acagtctgga tggatgggcc
      181 gttgaccgcg ggagtcaagc atggggccat tgtctatctc gacgaggtgg tggaagccag
      241 aaaggacacg accgtcatca tccatcctct cagcgacgac cggcgagtgc tcccgatcga
      301 gaaaaaaggc cagattgtgg aggccgccga cgagttcatg ctggtgatct cctacaaccc
      361 cggctatcag agtgtcatca aagatctgaa gcaaagtacg aaacaacgat tcatggcgat
      421 tgaattcgac tatcctgcgg cggacatcga agcgatggtg gttcagcgtg aggcgggggt
      481 ggatcctgcg atcgccggaa gtctcgtcaa gctcgggcaa aaggtccgca acttgagaaa
      541 ccacggattg gaggaaggag tcagtaccag actcttgatc tacgccggta ccctgatgaa
      601 gcaaggcgtg ccgcccgggc gggcctgtga tgtggccatc gctcgtccga tcacggatga
      661 ttcggacatg caacgggcga tactcgaatt tgtcaaagcg atcttttgaa tccatcgatt
      721 cccttcctgc catcgcacgg gacagtgagc gcggggcgct tctgaccgta catgtccaac
      781 ccggatcttc acgtactgag tgcgtcggaa ttcatggcga cgccataaag atccgcctgg
      841 cagctcgtcc gatcgacggt gcggccaacg acgaactgat tcgcttcatc gccgatcgct
      901 gtgctgtgcc gcgtgcaaat gtacaaattc gcgcaggagc cgaagctcgg cgcaaacggc
      961 tgtgtgtgaa ggagatctcg gctcaggtgc tcttggctcg attgctgccg cgaaacgagc
     1021 aagaaacgga gcggatatga agggattacg actcagcagc gccgtcttgg cagtggtgat
     1081 gctctcagct tgttccagcg cacgtccggt gctctatccc aatacccagc tggagtcggt
     1141 tggaaaagac ggggcagagc gtgatatcga agcctgccgg caattggcag aatcggctgg
     1201 tgcggcggaa ggtggtgggg gtaagggggg acgtgtcgca acgaacacgg ccgtcggcgc
     1261 cggagtcggt gccgcaagtg gagcggtagg aggagcgatt ttcgggggag tggggcgagg
     1321 atcgatgatc ggtgctgcga gtggagcggt ctggggcctc ctgaccggtt tgttccatgc
     1381 tgcgagtcct tcgcaaccca accaggccta cacgaatttc gtcaaccgat gtttgcaaga
     1441 gaagggatat gaggtgacag gctggtagtg agggggggcc acagcggaag ggtgggtact
     1501 cgtgctcgct gtccgcgcac gatgggaatg tgctcggaca atgcgcgcag aaagaaagat
     1561 tgaggcgagc gaaccaaatc gctgatgatg tgcacaattg aaaaaatctc gacatttact
     1621 tcaaagggtg agaagtgaag aaaggtaaat cctcaaagag ctttctgtgg tcaatcgctt
     1681 acggcattgt tgtattgtgt gcgcactact cgtaagtctt actgcctgcg ccggtccgca
     1741 gccgatctta cggtcgaata agcaactcca cttttatggc aagcagatgg ctcaacagga
     1801 ggttcaatcc tgtcggaaga aggttgaaga gaccggattg caacctggcg tgcaccagag
     1861 cgcgaatgcc gcgaccggcg ccatactcgg tctgacgctt ggcggcgctg tcggcgcgtc
     1921 ggcgggagtg gtcgggggat tacccggagt ggcgatcggg gccgccgccg gtagcgggct
     1981 tggattggtc gtcggcttac tcggaggaac cttcaagccg ctggaaccgg aacagccata
     2041 tgccgatgcc gtcgcgggct gtctgaagga aaagggctat gaagtcggcg ggtgggagta
     2101 gttccaaagg tcgttggatt cccatcagca gacgcaaggc atgccacgac cggctatcgt
     2161 cctcgcgagt ttacaaaacc cacagcggca tgtgacctcg acaagg
//
LOCUS       DATMTI010000178         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_49370, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000178 DATMTI010000000
VERSION     DATMTI010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49370"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            494..>3279
                     /locus_tag="VN039_02970"
     CDS             494..>3279
                     /locus_tag="VN039_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV44982.1"
                     /translation="MSHQKPPALSCIAVALAACLATPSAFSKPANNGSSSPSPRSTPK
                     PGQKDPGYQTSASTAKPPPPVKTTQLQRIVVTGTLLPINPNDAAVPVTTLDADNLRQT
                     GVTSNMLDALTKAIPAFAGRSNAGPSNAQNHNQFTAGGSQVELRNLPTLVLVDGQRMA
                     LDGVAGLSGSKNFVDISQIPAAALERVDVLTDGASSLYGSDAVGGVVNFILKRDYHGV
                     TVGTHYGVADGGYHDRSAFVTVGGDIGPVNITATVSDSKTSPLFESARSFTSPQYGVT
                     PGTGLPGVVDGGHYVLGPGLISPPVPIGTSATAGSYANLSPGVYNATSASQLSNRFDY
                     SKYAMLLQQEEHKNFVATINSQPFLDGRVTAFGDVMLSQNKVHSTAWQAAGQPFAFAS
                     LNVPAGAPFNPLTTTATGVTFADMSRPKGVFDTTDAYRFSAGLKGKFSHTWTWQTSVD
                     YSDSKVEERDTNLLFRPNLKAAVAGGYDANGNPVAGGAYSKVYGGYSIHNASMLQPAL
                     DPFAVTGNSAAALANIFGTEVLNGDSKLYSWDAHVAGDVFHLPAGPVSVAVGVGWRHE
                     GVSGHADPNGRVTDPVTGSTAGNAQNWIGGLYTDPFSHGRDVTAVYAETLIPIISGSM
                     HIPGLHRLDLTAAGRFEHYSDAGNGTSPKFGFRWEPFDNQFVVHATYTKSFAAPPLYQ
                     AYGPFDTRPVTDKLIGIVFGMPYAVGNTFNGQDGANPSLKPSTAQSRSIGFEFHPNFI
                     NGLTVTADYSDITVRGFPGGADFTRILQSVNDSGSGSPYFDSVSTGNFTNLGGTSPFG
                     KPGSLKAFLTNPATGQPDPSKYSQLYVIDKFRNQSVLVEHSWLLGLDYIFPWNKYGSW
                     ELSSKGTIFNSFKFQRRPGDAFQQYAGAASNIGVFAGTLPRYRFYSTLDWSLQNLDIT
                     LANTYVSSVQDKGAA"
BASE COUNT          688 a         1011 c          903 g          677 t
ORIGIN      
        1 acggcacggg atcgcgtcta tctggtcgcg gccgaagggg gcgtcgatcc ggccatgaaa
       61 gtgaataccg cggtggcacc gttctacccg aacttctatt tcgaccagac gttcaccgtg
      121 ttgacctacc aaaagaactg aatcttctga ttttcgtgtt aggggggggc gatcgaattg
      181 atacaaacgg cgagtccggg aagagattcc cgggagggtt tcaggcacaa cgtctaaatg
      241 tgagctcttg tatgtgcttt tcagcctgca tcgagcatcg atccaattgc tttccatgct
      301 gtcaagtgct ttggggatga ggggcatgcg gcataggcct actgactgaa tattggcatc
      361 cggacagggt tttcacgacg atcaattcat gtgagcgtga actacagact gctgatgctt
      421 ccgatcaacc gacgaatccg tgctgcgaaa tagagagaat tcattcgcag caccctgggg
      481 agggaatcag attatgtctc accaaaagcc acccgcattg tcatgcattg cagtcgcgct
      541 ggccgcctgt ctggccacac cgtcagcatt ttcaaaacca gcgaacaatg gcagttcttc
      601 accatcgccg cgaagcaccc cgaaaccagg ccaaaaagat cctggctatc aaacctccgc
      661 ttcgactgcc aagccaccac cgccggtcaa gaccacgcag ctccagcgaa tcgtcgtcac
      721 cggaacacta ttaccgatca accccaacga cgcggccgta cccgttacca ccctggatgc
      781 ggacaatctc cgccagactg gcgtcacgag caacatgctc gacgccctta ccaaggcgat
      841 ccctgccttc gccggacgta gcaatgccgg tccaagtaat gcgcagaatc ataaccagtt
      901 cacagccggt ggctcgcagg tcgagctacg caatttgccg acactagtgc tggtggacgg
      961 acagcgcatg gcgctggatg gggttgctgg cctgagcgga agcaagaatt tcgtcgacat
     1021 cagccagatc ccggccgctg cactcgaacg ggttgatgtg ctcaccgatg gcgcttcgtc
     1081 actctacggc tccgatgccg tcggcggcgt agtgaacttc atcctcaagc gcgactacca
     1141 cggtgtgacg gtcggcaccc attacggtgt ggcggatggg ggctaccacg atcgttcggc
     1201 gttcgtgaca gtaggcggag acatcggccc ggtgaacatc accgccaccg taagcgattc
     1261 caagaccagt ccgctgttcg aaagtgcccg ctcgttcacc agcccgcaat acggcgtcac
     1321 gcccggtacg ggcctgcccg gagtcgtcga cggtggacac tatgtacttg ggccggggct
     1381 gatctcgcca cctgttccga tcggcacctc ggcaactgcc ggcagttacg cgaatctgtc
     1441 tccaggcgtt tacaacgcta ccagcgcctc gcagttgtcc aatcgattcg attattcgaa
     1501 gtacgccatg ttgctacaac aggaggagca taaaaacttc gtcgcgacga tcaactcaca
     1561 gccgttcctg gacggtcggg tgacagcctt cggcgacgtc atgctctcgc aaaacaaggt
     1621 tcattccact gcgtggcagg ctgcgggcca gcccttcgcg ttcgcctcgc tcaatgttcc
     1681 ggccggcgcg cctttcaatc cgctgaccac caccgcgacg ggtgtgacgt ttgccgacat
     1741 gtcccggccc aagggagtat tcgataccac cgatgcgtat cgcttttccg ccggactcaa
     1801 gggtaagttc tcacacacgt ggacctggca gaccagcgtc gattacagcg acagcaaagt
     1861 ggaagagcgt gacaccaatc tgcttttcag gcccaatctg aaagctgccg tggctggggg
     1921 ctacgatgcc aacggcaacc cggtggctgg cggtgcctac agcaaggtct atggtggcta
     1981 ttcgatccac aacgcgtcga tgttacagcc ggcgctcgat cccttcgcag tgaccggcaa
     2041 ctcggcggca gcgctggcca acattttcgg caccgaggta ttgaatgggg atagcaagct
     2101 ttattcctgg gacgcccatg tcgccggcga tgtgtttcat ctacccgcgg gccccgtgag
     2161 cgtcgcagtc ggcgtgggct ggcgacatga aggagtttcc ggccacgccg acccgaatgg
     2221 ccgcgtcacc gatccagtca cgggttccac cgccgggaat gcccagaatt ggatcggtgg
     2281 cctctacacg gatccgttca gtcacggtag agacgtgacc gcggtgtacg ccgaaaccct
     2341 gatcccgatc atctcgggca gtatgcacat tccaggcctg catcgacttg accttactgc
     2401 tgcgggtcgc ttcgaacatt acagcgacgc cggcaacggc acctcgccga agtttggctt
     2461 ccgctgggag ccctttgaca accaattcgt ggtccatgcc acctatacga aatccttcgc
     2521 cgcaccgccg ctatatcagg cttacgggcc gttcgacact cgcccggtga ccgacaaact
     2581 gatcggtatc gtgtttggca tgccgtacgc ggttggcaat actttcaacg gccaagacgg
     2641 cgccaaccca tctctcaagc cgtccactgc acagtcgcgc tcgatcggct tcgaattcca
     2701 tccaaacttc atcaacggcc tgacagtgac tgcggactac tccgacatta cggtgcgcgg
     2761 ttttccgggg ggcgcggact tcacccgcat cctgcagtcg gtcaacgact ccggctcggg
     2821 ttcaccgtac ttcgacagcg tgagcacagg caatttcacc aatcttggcg gtaccagtcc
     2881 ttttggaaag cccggctccc taaaggcgtt cctgaccaac ccggctaccg gccagccgga
     2941 cccgagcaag tactcgcagc tctacgtcat cgacaaattc cgcaatcaga gtgtcctggt
     3001 cgagcattcc tggttactgg ggctggatta catttttcca tggaacaagt acgggtcctg
     3061 ggaattgtcc agcaaaggca cgatattcaa ttcgttcaaa ttccaacgtc gcccgggcga
     3121 tgcgttccag cagtatgcgg gcgcagccag caacatcggt gtgtttgccg gaacgctgcc
     3181 tcggtatcgt ttctactcga cactcgactg gtcgttgcag aatctggata tcacgctggc
     3241 caacacctac gtttcctcgg tccaggataa gggggctgc
//
LOCUS       DATMTI010000179         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_644129, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000179 DATMTI010000000
VERSION     DATMTI010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644129"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VN039_02975"
     CDS             <1..998
                     /locus_tag="VN039_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV44983.1"
                     /translation="TTLLRMIAGFNSIEGGTISFNDDVINNRPPKDRNIGMVFQNYAV
                     FPQMTVAKNVGFGLTNQKVPKAEAAQRVDKVLDAVHITQLKDRLPANMSGGQQQRIAL
                     ARAIVIEPAVLLMDEPLSNLDAKLRVEMRDVIKDIQHTVGITTVYVTHDQEEAMAVSD
                     RIAVMNNGQVQHAGTPVNIYHRPANVFVADFIGKANFLKRTLEPVGDRVLLALTPDYR
                     LDVTELLRIVPESGSGEVIVSLRPESISVSSTSEGEGMEVMILDGTFLGSSTHYRGEL
                     TTGEHIEFVDVSEIEAVMRRGMKAKLSIDVAKLNIFDASTEQSITVGVMNDAELD"
     gene            991..2640
                     /locus_tag="VN039_02980"
     CDS             991..2640
                     /locus_tag="VN039_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWV44984.1"
                     /translation="MIKKKRDIWFYAASLIFLFYIVIMVYPLVRLLGESFTGQAGASF
                     SLQYFVEFFTTPYYARALWNSLLVTVSVTVLTIVIAFPLAYFMTTIRIRGKNTLQILI
                     LISSMSPPFIGAYSWILLLGTNGVITNFAHNVLHIQIPTIYGFTGIVLVLTLQLVPLI
                     FMYLMGAWRTIDVSLLEASESMGVSGLRRMYKITGPLILPTILSGGLLVFMRALADFG
                     TPMLIGQGFQTVPVLIYNSFVSEVGTNDGLAAAISVVVIVVTLIIFLIQKYFASRKNF
                     SMSALQPILPKKAHGLKNIVGHAYVYGFIALAILPMAYVVYTAFLKTKFGVFIPGYSL
                     DSFTTAFNTSSNAILNTLVLGIVALACVVVIAVFVAYVSVRRRNALTGTLDTLTMVPY
                     IVPGLVVGIAFLLSFNRAPLLLTGTATIMILALVMRRLPYTIRSSSAILYQISPSVEE
                     ASISLGASNLKTFFRVTIPMMGPGVISGAILTWVAIITELSATIFLYTVHTETLSISI
                     YTEVIRGQYGVAAALSTILTVMTVVSLFIFLRINRDKEISI"
BASE COUNT          674 a          972 c          850 g          747 t
ORIGIN      
        1 agaccaccct gctccggatg atcgccggtt tcaactcgat tgagggcgga accatttcgt
       61 ttaatgacga tgtgatcaac aaccgaccgc caaaggaccg caacattggg atggtcttcc
      121 agaactatgc ggtgtttccg cagatgacgg tcgcgaagaa cgtcgggttc ggcctcacta
      181 atcagaaggt cccgaaagct gaagccgccc agcgagtgga caaagtgctt gatgccgtcc
      241 acatcaccca gctcaaagat cgcctaccgg caaatatgtc cggtggtcag cagcaacgga
      301 ttgctctggc cagggccatc gtcatcgaac cagcagtgtt gcttatggat gagccgctct
      361 cgaatctgga tgccaaactc cgggtcgaga tgcgggatgt catcaaagac attcagcaca
      421 cagtcggtat cacaacggtt tacgttacac atgatcagga ggaggctatg gcggtctccg
      481 atcggatcgc cgtgatgaac aatggccagg tgcaacacgc gggaacgcct gtaaacattt
      541 accaccggcc ggcgaatgtg ttcgtcgctg acttcatcgg caaggcgaac ttcctaaagc
      601 gcactctgga gcccgtcggc gatcgggtac tgctcgcctt gacccccgat tacaggctcg
      661 atgtcactga gcttcttcgg atcgtgccgg aatcggggtc cggtgaggtg atcgtttcct
      721 tgcgccccga gtccatctcg gtgtcgtcca ccagcgaagg tgagggtatg gaagtcatga
      781 tcctcgatgg cacattcctg ggctccagca ctcactatcg cggggagctc actaccggcg
      841 agcacatcga attcgttgat gtctccgaaa ttgaggccgt catgcggcgg gggatgaaag
      901 ccaaactctc cattgatgtg gcgaagctca acatctttga cgcctcaacc gaacagagca
      961 tcaccgtggg ggtgatgaac gatgcggaac ttgattaaga agaagcgcga catatggttc
     1021 tatgccgcca gcctaatttt cttgttctac atcgtcatca tggtctatcc gctcgtgcgg
     1081 cttctaggag agagcttcac cggacaggcg ggagcttcgt tctcgctcca gtacttcgtg
     1141 gagtttttca ctacgccgta ctacgcgagg gccttgtgga atagcctcct cgttaccgtg
     1201 agcgtcacag tgctcacgat tgtgatcgcg ttcccactgg catattttat gaccacgatc
     1261 cgtatccgtg gcaaaaacac gctccagatc ctgattctga tctcctcaat gtcaccaccc
     1321 ttcattgggg cctattcgtg gattctccta ctcggcacaa acggcgtaat caccaatttc
     1381 gcccacaacg tgctgcacat ccagatcccc accatctacg ggtttacggg aatcgtgctc
     1441 gttctaaccc ttcagctggt ccccctcatc tttatgtacc tgatgggcgc atggcgcacc
     1501 atcgatgtct cgctcttaga agcttctgaa tcgatgggcg tgagtgggct tcgcagaatg
     1561 tacaagataa ccggacctct gattttgccg acaattctgt ccggcggtct cctcgtcttc
     1621 atgcgagcgt tggccgactt tggtacgccg atgctgattg gccaaggctt tcagactgtc
     1681 ccagtgctca tctacaactc gttcgtgagc gaggtaggaa cgaacgacgg tctcgcggcc
     1741 gctatcagcg tggtcgtcat tgtcgtcacc ttgatcatct tcctgattca gaagtatttc
     1801 gccagccgca agaacttctc gatgagtgcg ctccagccga ttctgccgaa gaaggcgcac
     1861 ggcctgaaga acatcgtcgg ccacgcttat gtctatggat ttatcgcact tgcgattctg
     1921 cccatggcgt acgtggtgta cacagcgttc ctaaaaacaa agttcggcgt gttcataccg
     1981 ggctattcgc tcgacagctt cacgacagcg ttcaacacga gttcaaacgc gatcctaaac
     2041 acgcttgtcc tcggaatcgt cgcgctagcc tgcgtcgtag tcattgctgt ctttgtggct
     2101 tacgtctctg tccgtcgtcg gaacgcgctt accggcacgc ttgacacgct caccatggtt
     2161 ccgtacatcg tgccaggact ggtggtaggc atcgcattcc tcctgagctt caaccgagca
     2221 ccgctccttc tcacgggaac ggcaaccata atgatcctgg ccctggtaat gcgccgcctg
     2281 ccctacacga tccgatcgag ctcggcaatt ctgtatcaga tcagtccatc ggtcgaagaa
     2341 gcatcgatca gcctcggcgc ctcaaatttg aagacgttct tccgagtgac gattccgatg
     2401 atggggccag gcgtcatctc cggtgccatc ctgacctggg tcgcgatcat aacggaactt
     2461 agcgcaacga tcttcctgta cacggtccac acagagacgc tgtctatttc gatatatacc
     2521 gaagtcatcc gaggccaata cggagtagcc gctgccctct caaccatcct cacagtgatg
     2581 acagtggtat ctctgttcat cttcctaagg atcaacagag acaaggaaat ctccatctga
     2641 cccgcccggc gcccgggaat tagctcaatc agttgtgtag acgccctcaa cgctacccat
     2701 ctccgtcgct cctgcgaatc tgggaaaagg cgcttgagtg ggcacgaccc gcgcagaaca
     2761 ctatccatat tgtcagtcgt tacatctcgc agcggcgcgc ggccagtggt ttccccagct
     2821 gggatcccgg tgtgaacgtc cccccaacct tccaccggga tccctccctt ctgatacagc
     2881 caccgtgtca gcgctcctca cgagcgtttc tgctcaattc ctaacgagcg ccgacacacc
     2941 ggacatctca ccgccgctgg aaggcagcga gagttgttca cattgttccc gggtccgggc
     3001 cgatggcctc gtcagttcca ccgcacggtg aaccatccct ctacgggccg gcgagctccg
     3061 attccgacga cgagccagat gagcgcgctc gatgttgtcc tgccgctcac tgtgggctcc
     3121 cgagtagtgc gcccttggcg ttcaggaagt cgatgtgcag gtggttgccg gtgtcgtcga
     3181 tctggccgaa gttcgcggtg gcgatcgtca caccggcagc tgcacgaatc tccttctgcc
     3241 cga
//
LOCUS       DATMTI010000180         7011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_304816, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000180 DATMTI010000000
VERSION     DATMTI010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304816"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VN039_02985"
     CDS             <1..948
                     /locus_tag="VN039_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWV44985.1"
                     /translation="VAADPRRPAAGEQLSALGRDLGVEVHCADHVQASQADVVRICRA
                     GVERGRDQGFDLIILDTGGRLHIDDELMNELVAVKTDVSPHEVLLVADAMTGQDAVTM
                     AGQFDQKIGLTGIILTKVEGDARGGAVLSIRAATGKPIKFLGVGEKLDALEPFHPDRM
                     ASRILGMGDVLSLIEKAQESITREQAEEAQKRLTGNTFTLEDFRTQLGQMNRMGSFEQ
                     ILGMLPGGQKLKQAIEGDKPEREIGRVIAVIDSMTPRERRDHTIINGSRKKRIARGSG
                     TTVQDVNRLIKQFLSAKKLAKAMTGAGGRRQLAQLMQSR"
     gene            994..1287
                     /gene="rpsP"
                     /locus_tag="VN039_02990"
     CDS             994..1287
                     /gene="rpsP"
                     /locus_tag="VN039_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWV44986.1"
                     /translation="MAVHLRLARTGRHKRPMYRVVAADSRKARDGRFLEILGIFDPLK
                     EAGVPELKQERVLTWLRHGAQPTVTVRTLLRRAGVWKQFEAEKVAQKKASGKS"
     gene            1241..1813
                     /gene="rimM"
                     /locus_tag="VN039_02995"
     CDS             1241..1813
                     /gene="rimM"
                     /locus_tag="VN039_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWV44987.1"
                     /translation="MRLKKSRRKKRLENPDITMVYQQETVTVGRIERPFGIKGEVKVR
                     PLSDVPGRFEGLRSVSLLARNGQTLETNVTHVRRVGSEFILGLTGLTTPEDASLWRGG
                     FIQTIRGSVPKLPEGQYYECDLVGLAVFTGEGQPIGVLEEIWDLPGNPLFVVRQGAKE
                     ILIPAAKELISTVDLTARKMIVRLIDGLGE"
     gene            1815..2600
                     /gene="trmD"
                     /locus_tag="VN039_03000"
     CDS             1815..2600
                     /gene="trmD"
                     /locus_tag="VN039_03000"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWV44988.1"
                     /translation="MLRFEVLTLFPGMFAPVLAHSMLKRGQEKGLLTVKVHNLRDFTA
                     DRHKVVDDMPYGGGAGMVMKAEPILLAVAALRNEAQANGEDIRMMFPTPHGRPFTQDY
                     AQQLSGERRRIVILCGHYEGVDERVRLTLAPEEVSLGDYVLTGGELPALVLIDAAARL
                     VPGVLGDPSSVLEESFADTLLEYPQYTRPAEIRGIGVPDVLLSGHHEAIRLWRRKQSL
                     RSTYVRRPDLLKDRMFTKEDQQLLDELMSEGLLTAPTSRREEG"
     gene            2605..3009
                     /gene="rplS"
                     /locus_tag="VN039_03005"
     CDS             2605..3009
                     /gene="rplS"
                     /locus_tag="VN039_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWV44989.1"
                     /translation="MNQLERIQRSLTKKSVPSFEIGDTVRVHVKVVEGEKERIQVYEG
                     TVIARKGSLNTETFTVRKLSYGVGVERIFPVHSPIVSKVDVVRQGRVRRAKLYYLRGK
                     KGRFAKVEEREFVGESKPSAQPTASEKEAVTA"
     gene            3073..3732
                     /locus_tag="VN039_03010"
     CDS             3073..3732
                     /locus_tag="VN039_03010"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWV44990.1"
                     /translation="MGPTEEFERAARLCGYRRIAGIDEAGRGPLAGPVVAAAVVLPTR
                     CRLSGVDDSKQLSEGQRDRLYALILEQAVGMGIGSADVAEIDRLNILEATRLAMRRAI
                     DQLAPPPDYLLIDAVTLPGIRISVRPIIKGDSLSLSIAAASIIAKVTRDRLMAKYHEM
                     FPEYDFLSHKGYCTTGHLQRLARHGPCSIHRRTFMPVHEMIIPAKRESARRMLPTLFE
                     Q"
     gene            3737..4126
                     /locus_tag="VN039_03015"
     CDS             3737..4126
                     /locus_tag="VN039_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWV44991.1"
                     /translation="MATSDPRHQFGQASEMQAEQFLLAKGYRILDRNVRTPLGELDLV
                     AEDQGVVVFVEVKGRTTEAFGGALLAVNHRKRMKLAKLATLYLAQRHWSDKACRFDVV
                     LVQGRPSDRGMIEHLQNAFDVGEHGKF"
     gene            4147..4836
                     /gene="ftsE"
                     /locus_tag="VN039_03020"
     CDS             4147..4836
                     /gene="ftsE"
                     /locus_tag="VN039_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWV44992.1"
                     /translation="MDAIIQLIHVSKWHDRKAALSDVTLEIEKGEFVLLMGSSGAGKS
                     TLLRMLIGEEQPDEGQIFVHGRNVTKLRPAEVPYLRRKVGAVFQDFRLLPKKSVFDNV
                     ALPLIVQGASEKDVRRKVTEALRAVDVDHKKDQLPNSLSTGEQQRVCIARAIVNGPVV
                     LLADEPTGNLDPERTREIMELFKLINARGTTVIVATHDPHVLNHMNRRVITLVQGVLL
                     SERQIRQGIGV"
     gene            4833..5732
                     /locus_tag="VN039_03025"
     CDS             4833..5732
                     /locus_tag="VN039_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWV44993.1"
                     /translation="MRRLFYLIREASANMRINRTTTIVAVLTTAFTLACVGIFLLLYV
                     NLRHAAGLLQEDVKVMVYLDDRLPRERVQELERTLKIDRMVAEVFFISKEQALGEFRV
                     QFPSESHLLDGLGENPLPASFVVTLAPNFRSPDAMKGWVERVQTMEGVAKVDYNQEWL
                     SLLAELIGYIELAAIGVGILLSAAAVTIIGNTIRLALLARREEIEILRSIGATRTFIR
                     IPYFLEGAALGACGSALSLGILKVAFELFRQQIQLTGRFSGIGDMISFFPLSVCLALV
                     MAGMGLGFAGSVVSLLRVGQGRA"
     gene            5729..6910
                     /locus_tag="VN039_03030"
     CDS             5729..6910
                     /locus_tag="VN039_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWV44994.1"
                     /translation="MRISFILLWCLLVEGGWASVAGAANDPISEKIERQRKTLETLKD
                     KIEEKRKRVEEAEKKRESVLQGIQSLDERLVHHRQDHHEISKKLRQKDREIEEITDRL
                     GAMRASIDARREAILARLRVQYMEGRFGRVKVLLTADSYGDFQRRGQYLSAVSHKDYE
                     LLETFRADMERMQQAEHQRAEARAVLVAFKMNIEKKLADIRVLQKEKKVYLTKITHEK
                     DSYNRTVEELQRSASRVDGLLRDLETRRRATALHPPASSPLAHGVRGTLPWPADGKVV
                     SFFGRQKHPTFNTYVQRKGIEIRTAEGSLIHAVMTGSVVYADWLKGYGLVIILDHSNG
                     FFSLYAHASKILAKAGEQVAEGQPIGETGDTGMIGENTLYFELREGAEPVDPLHWLAK
                     R"
BASE COUNT         1582 a         1707 c         2187 g         1535 t
ORIGIN      
        1 gttgccgccg acccgcgtcg tccggcggcg ggtgagcaac tgagtgccct tggtcgggat
       61 ctcggagtag aggttcactg tgcggatcac gttcaggctt ctcaagcgga tgtggtaagg
      121 atctgccgtg caggggtaga gcgaggacga gatcagggtt ttgatctcat catcctggat
      181 accggaggcc gtttgcatat cgacgacgag ttaatgaacg aacttgtcgc cgtgaaaaca
      241 gacgtgtctc cccatgaagt gctgttggtt gccgacgcga tgaccggaca ggatgcggtc
      301 accatggccg ggcagtttga tcaaaagatc gggttgaccg gcattattct gaccaaggtc
      361 gaaggggatg cacgcggagg cgcggtgttg tcgattcggg ccgcaaccgg caagcccatc
      421 aagtttcttg gtgtcgggga aaagctggat gcattggagc cgtttcatcc ggaccgaatg
      481 gcttcgcgca ttcttgggat gggcgacgtg ctgtcgctca ttgagaaagc ccaagagagt
      541 atcactcggg agcaggccga ggaagctcag aagcggctca ccggtaatac cttcaccctg
      601 gaggatttcc ggacccagct cggtcagatg aaccgcatgg gttcgtttga gcagattctt
      661 ggcatgctcc cgggtggaca aaaactcaag caggcgatcg aaggggacaa gccggagcga
      721 gagatcggcc gtgtgatcgc cgtgatcgat tcaatgaccc cgcgggaacg tcgcgatcat
      781 acgatcatca atggaagccg gaagaagcgg atcgcccgtg gcagcggaac gacagtgcag
      841 gatgtgaatc gcctcatcaa gcagttcttg tccgccaaga agttggcaaa ggcgatgacc
      901 ggtgcgggag ggcgccgaca gctcgcccaa ctcatgcaat ctcgataagc gatcatgacc
      961 gatagtgcga attcagcgta aaggaggaca gttgtggctg tacatttgag actggctcga
     1021 acgggaagac acaaacgacc gatgtatcgg gtggtggccg ccgattcacg aaaagcgcgc
     1081 gacggacggt tcctggagat tcttggaatt tttgatccat tgaaagaagc cggcgtgccg
     1141 gaactgaaac aggagcgtgt cctgacgtgg ctgcggcatg gcgctcaacc cacggtgact
     1201 gtacggacat tgttgcgcag ggccggagtc tggaagcagt ttgaggctga aaaagtcgcg
     1261 cagaaaaaag cgtctggaaa atcctgacat cacgatggtg tatcaacagg aaaccgtgac
     1321 agtgggacgg atcgagcggc ccttcggcat caagggggaa gtgaaggttc gtccgctgtc
     1381 cgatgttccg ggccgatttg aagggttgag gagtgtgagt ctcctcgcaa ggaacggaca
     1441 gactcttgag accaatgtca cccatgtaag gcgcgtaggg tctgaattta ttcttgggtt
     1501 gaccggtttg accaccccgg aggacgcgag tctctggcga ggcgggttca ttcagacaat
     1561 tcgcgggtcc gtgcccaagt tgccggaggg gcagtactat gagtgtgatc tggtcggtct
     1621 tgccgtcttc accggggagg ggcagccgat tggtgtgctg gaggagattt gggacttgcc
     1681 gggaaatccc ctattcgttg ttcgccaggg ggccaaagag atcttgatcc ccgcagcgaa
     1741 agaactgatt agtactgtcg acctgacagc tcgaaaaatg atcgtccggt tgatcgacgg
     1801 attgggggag tagcatgttg cggttcgagg ttctgacatt gttccccggg atgttcgcgc
     1861 cggtcttggc tcacagcatg ctcaaacgag gccaagaaaa ggggcttctc accgtgaagg
     1921 tgcacaattt gcgggacttc acggcggatc gccacaaagt agtcgatgat atgccgtatg
     1981 gaggtggagc cggcatggtc atgaaggctg agccgatcct tcttgcggtt gctgcgctcc
     2041 ggaatgaagc gcaggcaaac ggtgaagaca ttcggatgat gtttccaact cctcacggga
     2101 ggccttttac acaagactat gcacagcagt tgtcaggtga gcgtcgccga atcgtcattc
     2161 tgtgcgggca ctatgaggga gtggatgagc gtgtccgtct gacattagct ccggaggaag
     2221 tctcactcgg agattatgtg ttgaccggcg gtgaattgcc ggcgcttgta ttgatcgatg
     2281 cagcggccag acttgttccc ggcgtcttag gtgaccccag ttctgtcttg gaagagtcgt
     2341 ttgccgacac gttgctggag tacccgcaat acacgaggcc ggcagaaatc cgaggaatcg
     2401 gcgtgcccga tgtcttgctc tcggggcatc atgaggccat tcgattgtgg cgccgaaaac
     2461 aatcgttgcg cagtacgtat gtgcgacgcc ccgatcttct gaaagatcgg atgttcacga
     2521 aggaggatca acagttgttg gatgaattga tgagcgaagg tcttttgacg gccccgacat
     2581 cacgccggga ggagggttaa gtccatgaat cagttggagc gaattcagcg gtcgttgacg
     2641 aagaagtcag tgccgagctt tgagatcggg gataccgtca gggtccacgt caaagtcgtc
     2701 gaaggcgaga aagaacgcat tcaggtctat gaaggaaccg tgatcgcgcg caaggggagt
     2761 ttgaatactg aaacatttac ggttcgaaaa ctttcgtacg gggtcggggt cgaacggatt
     2821 tttccggtgc attctccgat cgtctccaaa gttgatgtag ttcggcaggg gcgcgttcgg
     2881 cgcgcgaagc tatactatct gcgtggcaaa aaggggaggt tcgcgaaggt tgaagagcgc
     2941 gagtttgtcg gagagagcaa accgtcggca caaccgacgg cttctgagaa ggaggctgtg
     3001 acggcttagg ccgcttccgc gtatgtgttc cctcacggca atgcgatggc gcgatcggtg
     3061 aagggatgtt caatgggacc caccgaagag ttcgagcggg cggcccgcct gtgtgggtat
     3121 cgtcgtattg ccggtattga tgaggccgga cgcggtccgt tagccggtcc ggtcgtcgcc
     3181 gccgctgtcg tcttaccgac ccgatgtcga ctctcagggg tcgatgattc caaacaactt
     3241 tctgaagggc agcgagaccg attatatgcc ttaattcttg agcaagccgt gggaatggga
     3301 atcggatcgg cggacgtcgc tgagatcgat cggctcaata ttctcgaagc gacccggcta
     3361 gcgatgcggc gagccattga tcagctggct cctcctcctg attatctact cattgatgcc
     3421 gtgacgcttc ctggaatcag aatctccgta cggcccatca ttaagggaga ctccctttcc
     3481 ctctcgattg cggctgcctc catcatcgcc aaagtcacga gagaccgtct catggcgaag
     3541 taccatgaaa tgttccctga atacgatttt ctctcgcata aggggtattg cacgacagga
     3601 catctgcagc gattggcgcg tcacggcccg tgctccattc atcgtcgaac attcatgccg
     3661 gtgcatgaga tgatcatccc tgcgaaaagg gaatcggcca gacggatgct ccctacattg
     3721 tttgaacagt aagcgcatgg ccacttccga cccgcgccat cagttcggcc aagccagcga
     3781 aatgcaggcc gaacagttct tgttggccaa gggctaccga attctcgatc gcaacgtacg
     3841 gactcctctc ggcgaattgg atctggtggc agaagaccag ggtgttgtgg tctttgtcga
     3901 ggtcaagggc agaaccacgg aagccttcgg cggtgctctg ctggcggtga atcatcgcaa
     3961 gcgcatgaag ctggcgaaac tggccaccct atatttagcg caacggcatt ggtccgataa
     4021 ggcctgccga ttcgatgtgg tgttggtcca ggggagacct tccgaccggg gaatgatcga
     4081 acatcttcag aacgcgtttg acgtcggcga acacggaaag ttctaaccct tctccaaagg
     4141 gtttccatgg atgccatcat tcaactcatc cacgtgtcca aatggcatga tcgaaaagcg
     4201 gcgctgtccg atgtcacgct cgagatcgaa aagggcgagt ttgtcctcct catgggatcg
     4261 agcggggccg ggaagtctac gctgctgcgc atgctgatcg gtgaggaaca gcctgatgaa
     4321 ggacagattt tcgttcatgg caggaatgtg acgaagctca gaccggccga ggttccttac
     4381 ctacggcgaa aagtcggcgc ggtgtttcaa gactttcgtc ttttgccgaa gaaatccgtc
     4441 ttcgacaatg tggcgcttcc gttgattgtt caaggtgcct ccgagaaaga tgttcgtcgt
     4501 aaagtcacgg aggcattgcg cgcagtcgat gtcgatcaca aaaaagatca gttgccgaac
     4561 agtctctcca cgggagagca gcagcgtgtg tgcattgcac gggcgatcgt caacgggccg
     4621 gtggtgctcc tggccgacga accaacgggc aaccttgatc ctgaacgcac aagagagatt
     4681 atggagttgt tcaaattgat caatgccaga gggacgaccg tgattgtggc gacgcacgat
     4741 cctcatgttc tgaatcacat gaatcggcgg gtgattacct tagtgcaagg ggtgttgttg
     4801 tcggagaggc agatccggca agggatcgga gtatgagacg attgttttat ctcattcgcg
     4861 aagcgtcggc aaacatgcgg atcaatcgca cgacgacgat cgtggccgtt ctgaccacgg
     4921 catttacctt ggcctgtgtc ggcatctttc tgttgctcta tgtgaatctg cggcacgcag
     4981 cgggattgct ccaggaagac gtcaaggtca tggtttattt ggatgatcga cttcccagag
     5041 aaagggtgca agaactcgag cgaacactca agatcgatcg tatggttgcc gaggtgtttt
     5101 ttatttcgaa agaacaggcg ttgggagaat ttcgagttca attccccagt gagtctcact
     5161 tgcttgacgg gctcggtgag aaccctctcc ccgcatcgtt cgtggtgacg cttgcgccga
     5221 actttcggtc tccggacgcc atgaaaggct gggttgaacg agtgcaaaca atggaaggtg
     5281 tcgccaaggt cgactataat caggaatggc tcagtctgct ggccgaactg atcggataca
     5341 tcgagctggc tgcgatcggg gtgggtatcc tcctctctgc agccgcggtg acgatcatcg
     5401 ggaatacgat tcggctcgca ctgttggcca ggcgggagga gatcgaaatc cttcgctcga
     5461 ttggagctac gcgcacgttc atccgtattc catatttcct cgaaggagcc gcgctcgggg
     5521 catgcggcag cgcactgtcg ttgggaattc tcaaggtagc gtttgaattg ttccgacagc
     5581 agattcaatt gacaggccgt ttcagcggca tcggagacat gatttctttt tttccccttt
     5641 cggtgtgtct ggcgctcgtc atggcgggaa tggggcttgg ttttgccggt agcgtggtgt
     5701 cgcttctccg cgtggggcaa gggcgggcat gagaatctct ttcatcctcc tctggtgcct
     5761 tttggtggaa ggagggtggg catcggtagc gggtgcggcg aatgatccga tctccgaaaa
     5821 aattgagcgt cagaggaaga cgcttgagac gctgaaggac aagatcgaag agaaacggaa
     5881 acgggtcgag gaagcggaaa aaaaacgaga atcggttctg caaggtatcc aatctctgga
     5941 tgaacgactg gtgcatcatc ggcaagatca tcatgagatc agcaagaaac tgcgacagaa
     6001 ggatcgggag atcgaagaga ttacagacag gctgggggcg atgcgagcca gcatagatgc
     6061 gcgacgtgag gccattcttg cccggcttcg cgtgcagtat atggaaggac ggtttgggcg
     6121 tgtgaaggtt ctcttaacgg ccgactcata cggtgatttc cagcgtcgcg ggcaatacct
     6181 ctctgccgtt tcacacaagg actatgaatt gctggagacg ttccgagccg atatggagcg
     6241 gatgcagcag gccgagcatc agcgtgccga ggccagggcc gtactggtcg ctttcaagat
     6301 gaacatcgag aagaaactgg ccgatatccg agtgcttcag aaggagaaga aggtctatct
     6361 gacgaagatc acgcacgaaa aggattccta caatcgcacg gtcgaagaac tgcagcgttc
     6421 cgcctcgcga gtggatggtt tattgcgtga tttggaaacg cgccgacgtg ccacggccct
     6481 ccaccctccg gcgtcttcac ccttagccca tggcgtgaga ggaacactcc cttggccggc
     6541 cgatggaaaa gtcgtgtcgt tcttcgggcg tcagaaacac ccgacgttca atacgtatgt
     6601 ccagcgcaaa ggcattgaaa ttcggaccgc agagggaagc ttaatccatg ccgtcatgac
     6661 cggaagcgtc gtctatgcgg actggttgaa aggatacgga ctcgttataa tcttagatca
     6721 ctccaatggg tttttctccc tgtatgcgca tgcgtccaag attctggcca aagcgggtga
     6781 acaagttgcc gaaggtcaac ccatcgggga gacaggagac acgggcatga ttggagaaaa
     6841 tactttatac ttcgagttgc gtgaaggggc cgagccggtc gatccgctcc actggctggc
     6901 aaaacgatag gacgtgtgtt gtcggtgtcg atcgggtccg agccttcgac aacgcaggaa
     6961 agaagggatg tgaagattat ggaacagcag ccgcggcgga aatcttgggt g
//
LOCUS       DATMTI010000181         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_261740, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000181 DATMTI010000000
VERSION     DATMTI010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261740"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VN039_03035"
     CDS             complement(<1..602)
                     /locus_tag="VN039_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44995.1"
                     /translation="MSTITITEDGLLNVAYPAQPDEVHIVADIVGEKWTITGNGTTGD
                     LTATDGATGVLEIWTLIDPAGTPWTITLDADGVLHLQSFDTNIVLAPAIHTPIKVNGQ
                     LATGYQIFVYQQGTTTPATIYRNASSVSILEQPILINEFGLPHDPIFIQVGLAYDITI
                     VPPGGGNAVRSIPSVVGGIPINIPTATEWSGGSYFASLVD"
     gene            complement(599..1468)
                     /locus_tag="VN039_03040"
     CDS             complement(599..1468)
                     /locus_tag="VN039_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWV44996.1"
                     /translation="MGTQISPFGNNQFFTPGGVAAVGYQLFVYAGRSTDKARVWTDQD
                     GVVPHTNPIVLDANGFPPAPIYIDTTKAYKFVMAFPEDLDPPTLPLYTVDQVSVGLET
                     PTVSTPEWSTGPTPTYVGATQFSVLGNAIIFFPVGRRVKALINSGNLYGTITDVVYST
                     VTTVTVQWDSGNLDNTLSAIWFSFLNSQGSSWPSGYSDGLTTNFAGPVSIPVTSAFNL
                     MQAGSIFAFAGTATPPAGYLQCNGAAISRTQFAALFSVIGTAYGTGDGVLTFNVPSIA
                     NLISGVGYIIRYA"
     gene            complement(1468..>2658)
                     /locus_tag="VN039_03045"
     CDS             complement(1468..>2658)
                     /locus_tag="VN039_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV44997.1"
                     /translation="NPSSTNAEDFLVQKGARAAGGALAGAGIGALANKLTRAATPQAP
                     SLAGIEQEAKRAGFTGKAELTPAQRTGDQELQQYEEGLLSSPGSQKLIRERRNAQQDV
                     LNKAASKILGYPKMDPTEAAFGMARDNANLAYEPIAKIPKMSPDVPYWDALTNFAKKQ
                     AVKATGSTDAAGVANRLKKGAGKVTGQDFLEELQGVRDMAFGASKRGDTATAGQLKDL
                     SSIMEDFLDRKLTKLATQPGNMITTDTLKQYKDARLQHSLIHSLEKATDPVLGRVNPN
                     KILKEQFARQRPGSTASPTTKALQEVSNIARVMRKTMPYIGSSGTAERLGGQRMVEAE
                     LNPMASLRMAGPMLKNYLAAKMYLRNGGQPGMLGARLSPGQSALVRRLLPPQSIGTGE
                     ALAE"
BASE COUNT          526 a          741 c          812 g          579 t
ORIGIN      
        1 atatcgacca aggaggcgaa ataggacccg cctgaccatt ccgtggccgt tgggatattg
       61 atcggaatcc ccccgacgac ggacggaatg gagcgcacgg cattcccgcc gccaggcggc
      121 acaatcgtga tgtcataggc caggccgact tggatgaaaa tcgggtcgtg cggcaagccg
      181 aattcgttaa tcagaatcgg ctgctccaaa atggagaccg aggaggcatt gcggtaaatg
      241 gtcgcgggcg tcgtcgtccc ctgttgatag acgaagattt gatagccggt ggcgagctgt
      301 ccattgactt taatcggcgt atgaatcgcg ggagcgagga caatgttggt atcgaagctt
      361 tggagatgca gcaccccatc ggcatcgagg gtaatggtcc agggcgttcc agccggatcg
      421 atgagcgtcc agatttccag cacaccggtt gcgccatcgg tcgccgtgag gtcacccgtc
      481 gtgccgttgc cggtaatcgt ccatttctcg ccgacgatat cggcgacaat gtgcacctcg
      541 tccggctggg ccggataggc gacattcaag agcccgtctt cagtgatcgt aatcgtgctc
      601 atgcgtaccg aatgatgtag cccacgccgg aaatcagatt ggcgatactg ggcacgttaa
      661 aggtcaggac cccatctcct gtgccgtagg ccgtcccaat gacggaaaag agcgcggcga
      721 attgggtccg gctgattgcg gccccgttac attgcagata cccggcgggc ggtgtggccg
      781 ttcccgcaaa ggcgaaaatg ctgcccgctt gcatcagatt gaatgcggag gtgacgggaa
      841 tggagaccgg cccggcaaag ttggtcgtga ggccatcgct atagccggac ggccaggaac
      901 tgccttgaga attcaaaaag ctgaaccaaa tggccgagag cgtgttatcc agattgccgc
      961 tatcccactg cactgtgacg gtcgtgactg tggagtacac aacatctgta atggtgccgt
     1021 agagattgcc ggaattaatc agcgccttga cgcgacgtcc gaccgggaaa aagatgatcg
     1081 cattccccaa gacggaaaac tgtgtggctc ctacataggt cggagtcggg ccggtagacc
     1141 attccggtgt actgaccgtg ggcgtttcga gaccaaccga cacttgatcg accgtgtaga
     1201 gcgggagtgt cggcgggtcc agatcttcag gaaaggccat gacgaatttg taggccttcg
     1261 tggtatcgat atagattggt gcggggggga acccgttggc gtcgaggaca atcggattgg
     1321 tatgaggcac gactccatct tgatcggtcc acacccgtgc tttgtccgtc gaacgtccag
     1381 catagacaaa gagctgatag cccacggcag ccacgccgcc aggggtgaag aattggttat
     1441 tgccgaatgg agaaatttgc gtacccatta ctctgccaag gcctccccgg taccgatcga
     1501 ttgaggcggc aagagccgac gtaccaacgc actctgcccc ggggagagcc gagccccgag
     1561 catccccggc tgtccgccgt tccggaggta catcttcgcg gccagatagt tcttgagcat
     1621 cggtccggcc atgcggagcg aggccatcgg attcaattcc gcctcgacca tccgttgacc
     1681 accgaggcgt tccgccgttc cggatgagcc gatatagggc atcgtcttgc gcatgacccg
     1741 ggcaatgttc gacacctctt gcagcgcttt ggtcgtggga gatgctgtgc tgcctggacg
     1801 ttgccgagca aactgttcct tgaggatttt gttcggattg acgcggccaa gaacggggtc
     1861 cgtggctttc tcaagagaat ggatgagcga gtgttgcaga cgcgcatcct tgtactgctt
     1921 gagggtatcg gtcgtaatca tgttgccggg ctgcgtagcc agcttggtga gcttccgatc
     1981 gagaaagtcc tccataattg aagacagatc cttgagctgc cccgctgtcg cggtatcgcc
     2041 gcgcttactg gccccgaagg ccatgtcacg taccccctgc aattcctcca gaaagtcttg
     2101 accggtgacc ttccctgccc ctttcttgag gcgattcgcc acgcctgcag catcggtgct
     2161 gcccgtcgct ttcacagctt gcttcttggc gaaattggtc agcgcgtccc aatagggcac
     2221 atccggagac attttcggaa tcttggcgat gggctcatag gctagattcg cgttgtcgcg
     2281 ggccatgccg aaggctgcct cggtcgggtc catttttggg tagcccaaga ttttcgaggc
     2341 cgctttgttc aacacgtctt gctgggcgtt gcggcgctca cggatcaact tttgtgagcc
     2401 tggtgacgag agcaggcctt cttcgtattg ctggagttct tgatctccgg tgcgctgagc
     2461 cggagtgagt tccgccttgc cggtgaatcc ggcacgtttc gcttcttgct cgatacccgc
     2521 gagtgagggc gcttgcggtg tggccgctct ggtgagctta ttggccagcg ctccaatgcc
     2581 agcccccgca agggccccgc ctgccgcccg cgcacccttt tgcacgagaa aatcctcggc
     2641 attggtgctg ctcggatt
//
LOCUS       DATMTI010000182         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_134880, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000182 DATMTI010000000
VERSION     DATMTI010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134880"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..330)
                     /locus_tag="VN039_03050"
     CDS             complement(91..330)
                     /locus_tag="VN039_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWV44998.1"
                     /translation="MARCCWKRFLVPGKMTQAALAQKLGWTRARLNELIKGKRDITAD
                     SALDLARVLGTSAKLWMNLQATFDLDKAMRRRKIA"
     gene            complement(389..1351)
                     /locus_tag="VN039_03055"
     CDS             complement(389..1351)
                     /locus_tag="VN039_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HWV44999.1"
                     /translation="MTAQLQRTLRLAGIDIGTLTCRLLIADLAPGQPLQEIRSDRRIL
                     RLGEGVDQTKRLSPAAMDRVIHCLQEWRNVIHDFHVETSSIVATSAVRDATNRSEFLE
                     RVKRETGFDVEVISGEEEARRTLLGIRSGLPVGVTDILALDIGGGSTEFILDRQGQKP
                     IIRSIDIGVVRLCERLLTHDPPTDVEVHQAREWVARETKAAVAGMGNYRQATFVGTAG
                     TITSLAAMAQKLPAYEPTRIHNYQLKLETIRELEQTLFSRTKAERVGLPGLERDREEV
                     IAAGAIIIGTIMETLGQQGVLVSDLGLREGVLIDRAVRESMNKN"
     gene            complement(1348..1671)
                     /locus_tag="VN039_03060"
     CDS             complement(1348..1671)
                     /locus_tag="VN039_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45000.1"
                     /translation="MGFLQRLKDDLRAGITTLRLGTVHAAGRALEETELLRMRLELRK
                     LDQQLSDLYKDIGERAVDMKERGETAERVVYDAEIVRLVKEVEVLKESRKKLEADMEE
                     LRNEQ"
     gene            complement(1679..>2774)
                     /locus_tag="VN039_03065"
     CDS             complement(1679..>2774)
                     /locus_tag="VN039_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWV45001.1"
                     /translation="LVRGGEKSSVKKAQAERLASGDIQVAIGTHALIQQGVRFKNLGL
                     AVVDEQHRFGVLQRKTLIEKGYKPDVLVLTATPIPRTLAMTVYGDLDVSMIDVLPPGR
                     KPVHTFLFNDTQRRRATQIVRDELRAARQAYVVYPLVEESEKTDLQDAIQGAEQLRNG
                     EFAEFRVGLLHGRMKAAEKEAVMADFKAGTIQVLVATTVIEVGVDVPNATVILIEHAE
                     RFGLAQLHQLRGRVGRSNQQSYCLLMAQNLARGRTRLGRRSMGSEESVSTARERLEAL
                     VRSNDGFVIAEDDLRIRGPGEFLGLRQWGMPEFRVANLVRDGDLLQQARQEAFSLLKL
                     DPGLKEPAHQGLRDAMLRKWEKKLDLGSIS"
BASE COUNT          581 a          869 c          658 g          666 t
ORIGIN      
        1 acagagcaac tagcagggcc cccaaagcga ttagcatttg tagtattcat gtcgaggctc
       61 tttttcatac tggccgccaa cacttacagg tcaggcaatt tttctccggc gcatagcttt
      121 atcgagatcg aatgtggcct ggaggttcat ccagagcttg gcagatgtcc ccagtacacg
      181 cgcaagatcc agcgccgaat ctgccgtaat atcgcgtttc cctttaatca actcgttcaa
      241 gcgtgccctt gtccagccaa gcttctgcgc caacgctgct tgagtcatct tgcctggaac
      301 gagaaacctc ttccagcagc atctcgccag gacgaaacgg gttttgggga agtctcatga
      361 tgcctgtctc ctgcttacca aaactggctc aatttttatt catagactct cgcaccgccc
      421 gatcgatcaa cactccctct ctcaacccaa gatcgctgac aagcacacct tgctgcccta
      481 acgtttccat gattgtcccg atgatgattg caccagcggc gatgacttct tctcggtccc
      541 tttccaaacc tggtagacca actcgctcgg cctttgttct gctgaacagc gtctgttcca
      601 attcgcgaat tgtttccagc ttcaattgat aattatggat acgtgtcggc tcataggcgg
      661 gaagcttctg ggccatggct gcgagggacg taatcgtgcc cgcggtaccg acgaatgtcg
      721 cttgacgata gttacccatg ccagcaacag ccgccttcgt ctctctggca acccactcgc
      781 gcgcctgatg cacctccaca tcggttggag ggtcgtgagt caagagccgt tcgcaaaggc
      841 gaacaactcc aatatcgatc gagcggataa ttggcttctg tccttgccga tccaaaataa
      901 attcagtact tccgccaccg atatccaatg cgaggatgtc cgtcactcca accggtaagc
      961 cggagcgaat accgagcaac gttcgacgtg cttcttcctc ccctgaaatc acctcgacat
     1021 caaatccggt ctctcgtttg actcgctcaa gaaattcgct ccgattcgtt gcatcgcgga
     1081 ccgcgctcgt cgccacgatc gaagatgtct cgacatgaaa gtcgtggatg acattccgcc
     1141 attcttgtag acagtggatg acccgatcca ttgcggctgg actcagacgt ttggtctgat
     1201 cgactccttc gccgaggcgg agaatgcgcc ggtcagatcg gatctcttgg agaggctgtc
     1261 caggcgctag atctgcgatt aacaaacgac aggtgagggt gccgatgtcg atgccagcca
     1321 agcggagcgt gcgctggagt tgcgcggtca ttgctcgttc cgaagttctt ccatgtcggc
     1381 ttccagtttc ttcctcgact ccttaagcac ctcgacctct ttgacgagac gcacaatctc
     1441 ggcgtcgtac acgacccgct ccgctgtttc gccccgctcc ttcatgtcga cggcgcgctc
     1501 gccgatgtct ttgtacagat cagaaagttg ctggtcgagc ttacggagct ccagcctcat
     1561 gcggagcaat tcggtctctt ccaaagcacg gccggcagca tggacggtcc ctaggcgcag
     1621 ggtggtaata ccagctcgca agtcatcttt cagccgctga aggaatccca tcgatgtcct
     1681 agcttatcga tccaaggtcg agctttttct cccatttcct caacatcgcg tcacgcaacc
     1741 cttgatgggc cggctctttc agacctgggt ccaacttcag taacgaaaag gcctcctgcc
     1801 tggcctgctg caatagatcc ccatctcgca ccagatttgc gacacgaaac tccggcatcc
     1861 cccattggcg caaccccaag aactctccgg ggcctctgat ccgcaaatcg tcctcggcaa
     1921 tcacaaatcc atcgtttgac cgcacgagcg cctccagccg ttcccttgcc gtagacacgg
     1981 actcctcact tcccatggaa cgtcttccca gccgtgttct cccccgtgcc agattctgag
     2041 ccatcaaaag gcaataggac tgctggttgc ttcgccccac cctcccccgc aattggtgca
     2101 gttgtgcgag accaaaccgc tcggcatgtt cgatcaagat gactgtcgca ttcggcacat
     2161 cgactccgac ttcgatcacc gtggtcgcaa ccaatacctg gatcgttccg gccttaaagt
     2221 cggccatcac cgcttccttt tcggcggctt tcatgcgccc atgcaacagg ccgacgcgga
     2281 attcggcaaa ttctccgttc cgtaactgtt cggctccctg aattgcgtct tggaggtcgg
     2341 tcttttccga ttcttccacc agaggataga ccacatacgc ttgtcttgcg gcacgcaatt
     2401 catcacgcac aatttgagtg gcacgccgtc gctgtgtgtc attaaagaga aatgtatgca
     2461 ccggctttcg tccaggaggc agtacatcga tcatcgatac atccaggtca ccatataccg
     2521 tcatcgccaa tgtccgggga atgggcgtag ccgtcaagac aagcacgtcc ggcttgtaac
     2581 ccttctcaat cagtgtcttt cgctgcagga cgccgaacct gtgctgctcg tcgaccacgg
     2641 ccaaccccaa gttcttgaat ctcacccctt gttgaatgag ggcatgagtg ccaattgcta
     2701 cctgaatgtc gcccgatgcc agccgctccg cctgtgcctt ttttaccgaa gatttctctc
     2761 ccccacgcac gaga
//
LOCUS       DATMTI010000183         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_346986, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000183 DATMTI010000000
VERSION     DATMTI010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346986"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1560)
                     /locus_tag="VN039_03070"
     CDS             complement(88..1560)
                     /locus_tag="VN039_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWV45002.1"
                     /translation="MLADRLARYCRSLRYGDLPDTVAHEVKRRMLDSLGCALGAWNAP
                     PCRVARLIAQTVKVPQGATLWGTGHKTLPDLAAFANGGLVRYLDFNDTYLSKEPAHPS
                     DNIPAILSVGETVHASGKRVMEAIALAYEVQCRLCDASALRPRGWDHVTYGPFSSALG
                     VAKLMKLSNEQTVQAINLAGISNMALRQTRVGDLSMWKACAFSNAARNGVFAAMLAQL
                     GMTGPSPIFEGEKGFMKLVSGPLELAPFAAERGQGPRDWLRTEAGACPVPKGTDPSGR
                     GSVPETFKILDTYIKHYPVEYHAQTAVEAAIALRNELMEVEGTRAVDHVIDVEIGSYD
                     VAIEIIGRDPEKWQPATRETADHSFPYCVATALLHGHVTLQSFSPKRLHDPAVRNLMK
                     KVRVVQQPEFEGRYPKTMPTKITVKTEAGQTYMRQVDVPVGHPGSPMSDRDLEAKFRR
                     LAAGRLNRPHVDRFIEFVWNLDRVRDIGTLMPLLRMKGRG"
     gene            complement(1614..>2070)
                     /locus_tag="VN039_03075"
     CDS             complement(1614..>2070)
                     /locus_tag="VN039_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV45003.1"
                     /translation="GLELALACHIRVAAAGAMLGLPEIKLGLIPGFGGTQRLPRIVGP
                     SKAAEMILTGKSVSAEEALRIGLLSRVVPLHELITQVEAIAASITAHGKIAVEAALHA
                     IRGGIDIPLSEGLAREAELFGRLCATSDKHEAAQAFLEKRQPKLAETEA"
BASE COUNT          423 a          639 c          561 g          447 t
ORIGIN      
        1 cccgtagcca acagttcacg caaacgggat gtcttcgtag agctctttcc ctccatcatc
       61 gctgttattt ctcctgaatc ttaaacctta gcctcggcct ttcatcctta gtagcggcat
      121 gagtgtgcca atgtctcgaa cccgatcaag gttccacacg aattcgataa accggtcgac
      181 gtggggacga tttagccgtc cggcggctaa ccggcgaaac tttgcttcca gatcacgatc
      241 cgacatcgga gatccgggat gtcccaccgg tacatcgacc tgtctcatgt aggtttgacc
      301 tgcttccgtc ttgaccgtga tcttggtcgg catggtcttg gggtaacgcc cctcgaattc
      361 gggctgctgc accacacgga ccttcttcat tagattccgc acggccggat cgtgcaatcg
      421 tttcgggctg aatgactgca gcgtcacatg accgtggagc aatgccgttg cgacgcaata
      481 ggggaaacta tgatcagccg tctccctcgt ggccggctgc catttttcag gatcgcgccc
      541 gatgatttcg atggccacgt cataactccc gatctcaaca tctatgacat gatcgacagc
      601 acgagttcct tctacttcca tcagttcatt gcgaagtgcg atggcagcct cgaccgcagt
      661 ctgcgcgtga tactcgaccg ggtaatgctt gatataggta tcgagaatct tgaacgtctc
      721 agggacagac ccccttccac tggggtctgt ccccttcgga acgggacagg caccggcctc
      781 cgtccggagc cagtccctcg gcccctgtcc ccgttctgca gcgaatggcg ccagctccag
      841 tggccccgaa accagtttca tgaagccctt ttcaccttcg aagataggcg acggtcccgt
      901 catgcccaac tgcgccagca tggcggcgaa cacaccgttg cgtgcggcgt tcgaaaaggc
      961 gcaggctttc cacatggaca aatccccgac ccgcgtctgc cgaagggcca tattcgaaat
     1021 gccggccaga ttgattgcct gcaccgtctg ttcgttggaa agcttcatga gcttggcgac
     1081 accgagggca gaagagaagg ggccgtacgt cacgtggtcc cacccgcggg ggcgtagcgc
     1141 cgaagcgtca cagagacggc actggacttc ataggccagc gcgatggctt ccatgacgcg
     1201 tttaccggac gcatggacgg tctcgccgac cgaaagaatg gccggaatat tatcggaagg
     1261 atgcgccggt tctttcgaaa gataggtatc gttgaaatca agatatcgga ccagtccgcc
     1321 gttggcgaag gccgcaagat cgggcagggt tttatgaccg gtaccccaca atgtggcgcc
     1381 ctgcggcact ttgacggtct gggcaatcag acgggcgact cggcatggcg gcgcattcca
     1441 cgccccaagc gcgcacccaa ggctgtcgag catgcgccgt ttgacttcgt gcgcaacggt
     1501 atccggtaaa tcaccatagc gcagagaccg gcaataccga gcgagacgat ctgcgagcat
     1561 cggctgcccc ttttggatct atgccgtccc ctcacctctc acgcttcacg agttcatgct
     1621 tccgtttcgg ccaatttcgg ctgccgtttt tccaaaaatg cctgcgctgc ttcgtgtttg
     1681 tcggaagtgg cgcacaatct gccgaataat tccgcttccc tggccaggcc ttccgacagt
     1741 gggatgtcga ttccccctcg gatcgcatga agcgcagctt cgacggcaat tttcccgtgc
     1801 gcggtaattg aagcggcaat ggcctcaacc tgtgtgatca actcatgcag cggcaccacc
     1861 cgactcagca gaccgattcg caacgcctcc tctgcggaga cactcttacc cgtcaagatc
     1921 atttccgccg ccttggaagg cccgacgatt cgcggcaacc gctgagttcc tccgaagcct
     1981 gggatgagac cgagtttgat ttcaggaagt cccaacattg cgcctgcagc cgccacccga
     2041 atgtggcagg ccagagcgag ttcgagacca
//
LOCUS       DATMTI010000184         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_261845, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000184 DATMTI010000000
VERSION     DATMTI010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261845"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VN039_03080"
     CDS             complement(<1..256)
                     /locus_tag="VN039_03080"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV45004.1"
                     /translation="MSHSNKFVIRTYHRIPVRCVVYYLGGDFLGKGTVMNLCRNGFRV
                     LGDHQVAPGMQLVVRLTLPDKDEPVEIQRVVVRWVRGLLFG"
     gene            466..780
                     /gene="rplU"
                     /locus_tag="VN039_03085"
     CDS             466..780
                     /gene="rplU"
                     /locus_tag="VN039_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWV45005.1"
                     /translation="MYAIVETGGKQYRVETGTTIQVERLPGDIGAQIELSKVHLVHGN
                     DGILVGQPIINGAKVTAEIVRQGRTRSITVFKKKRRKNYRRTRGHRQGFTQLLIRNIA
                     TA"
     gene            802..1065
                     /gene="rpmA"
                     /locus_tag="VN039_03090"
     CDS             802..1065
                     /gene="rpmA"
                     /locus_tag="VN039_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWV45006.1"
                     /translation="MATNKGGGSSRNGRDSNPQYLGVKAYGGQTVTAGSIIVRQRGTK
                     FFPGFNVDLGRDHTLFAKMSGVVKFEGGRGRRKVSVYPVPVKS"
     gene            1194..2219
                     /gene="obgE"
                     /locus_tag="VN039_03095"
     CDS             1194..2219
                     /gene="obgE"
                     /locus_tag="VN039_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWV45007.1"
                     /translation="MFVDEVHITVQAGRGGNGICSFRREMFVPRGGPDGGDGGDGGDI
                     VMTASHRLTTLLDLRYQNHYEAQDGRAGGGSNCTGRSGGDLTIAVPVGTIVYDDLTKE
                     TLADFIADGQTAVMAQGGRGGKGNSNFATSVNRVPTKCTPGTPGEERTLRLELKLLAD
                     VGLVGFPNAGKSTLIAAISAARPKIADYPFTTLVPNLGVVRWGSDRSFVVADIPGLIE
                     GAHEGKGLGVQFLRHIERTAFLLHLIDVSEWAPDDPVIALETLRRELAAYDLGLAKRP
                     FAVVPTKIDVIGTSERLTQMQSYCCLNDYPCLPISAATNKGLDDMITYLGKRVDHLRK
                     TPCETSS"
     gene            2201..>3085
                     /gene="proB"
                     /locus_tag="VN039_03100"
     CDS             2201..>3085
                     /gene="proB"
                     /locus_tag="VN039_03100"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWV45008.1"
                     /translation="MRDKLLVQAKRIVIKIGSSLIASRAEGLRPQQIERLADEIAMLR
                     TQGLEILVVSSGAIVSGIRKLQLKDYPKSLPVKQAAAAVGQSRLMWAYEKAFERLNIQ
                     AAQILLTHQDLADRRRFLNARHTLTALIGFGIVPIINENDTVAVDEIRVGDNDTLASE
                     VAHLADADLLVILSDVDGLYTEDPRKNPSATLIPLITEITQDIERLAGVSSTFEGTGG
                     MATKLRAAKKVGEYGIPTLILNGERAGLLPTVIGGGPGGSLFLARERRLNSRKHWIAF
                     TLRSRGQVHLDQGAVDALT"
BASE COUNT          721 a          874 c          846 g          644 t
ORIGIN      
        1 cgccaaacaa taggccgcgc acccatcgca caacgactcg ctggatttcg accggttcat
       61 ctttgtcggg aagggtgagc cgaacgacca gctgcatccc gggagccact tgatgatcgc
      121 ccaggacacg gaatccgtta cgacataggt tcatgaccgt tccttttccg agaaaatccc
      181 cgcccagata gtacaccacg cagcgaacgg gaatccgatg ataggtccgg atcacaaact
      241 tattagagtg agacatgagc tggttgccat ctcccaattt gtgtcgcgag atactggacg
      301 atcgcgatga tggcgccaag catacttgtc ggcacgacac ccctcatagc cttctaaaga
      361 ggggcctatt gggtgatcaa tgatctccgg tcgctcctcg tttggcttga caccccccat
      421 atccccatgt tacatggacg actccttact ctcggagaat agattatgta cgcgatcgtt
      481 gaaacaggcg gcaaacaata tcgagttgaa acagggacga cgattcaggt cgaacggctg
      541 ccgggagaca ttggggccca gattgaactc agcaaggtcc atctcgtaca tggcaatgac
      601 ggtattctgg ttgggcagcc cattatcaac ggggccaagg tcacagccga aattgtgcgg
      661 caaggacgga cccgatcgat taccgttttt aagaagaagc gccgcaagaa ctatcgccgc
      721 acccgtggtc accgacaagg attcacccaa ctgttgatta ggaatattgc gacggcctaa
      781 agacacagga aggacttacc catggcaaca aacaaaggcg gcggatcatc acgtaacggc
      841 cgtgatagca atcctcaata tttgggcgtg aaggcctatg gtggtcagac tgtgacggcc
      901 ggcagcatta ttgttcgcca gcgtggcacc aagtttttcc ctggattcaa tgtggacctt
      961 ggaagagacc ataccttatt tgccaaaatg agcggtgtgg ttaagtttga gggtggacgc
     1021 ggcagacgaa aagtcagcgt atatcctgtt ccggtcaaat cctgattctc ttttctgcct
     1081 cctctgttac gatcacacag gataaattct cctctcacct cggggcacgg gtcggatata
     1141 cttttctcat cagcaggtac cggagttacc catctgtgga acgttgacag attatgtttg
     1201 tcgatgaagt acatatcacc gtacaagccg gtcgcggtgg aaacggcatc tgcagtttcc
     1261 ggagggagat gtttgttcca cggggaggcc ccgacggcgg ggatggaggc gacggcggcg
     1321 acatcgtcat gactgcatct catcgattga cgaccctgct tgacctccgc tatcaaaatc
     1381 actatgaggc tcaagacggg cgagccggtg gaggatctaa ttgcacgggc cgttccggcg
     1441 gagatctgac catcgctgta cctgtcggca ccatcgtcta tgatgacctg acgaaagaaa
     1501 ctcttgcgga tttcatcgca gacggccaaa cggccgtcat ggcccaagga gggcgaggcg
     1561 ggaaaggcaa cagcaacttt gccacatccg tcaatcgcgt accgacgaag tgcactcccg
     1621 gaactcctgg tgaagaacgg accttgcggc ttgagctgaa actgctcgcc gacgtcgggc
     1681 tggttggctt tcccaacgcc ggcaaatcga cgctcattgc agccatctca gcggcccgac
     1741 caaagatcgc cgactatccc ttcaccactc tggtccccaa tctcggagtt gtccggtggg
     1801 ggtccgaccg aagctttgtc gtggctgata ttcccggcct gatcgagggc gcccacgaag
     1861 ggaaaggcct gggcgtacag tttctccgcc atatcgaacg caccgcgttc ttgctccact
     1921 tgatcgatgt gtcagaatgg gctcctgacg acccggtgat cgctcttgag accttacggc
     1981 gggaacttgc cgcctatgac ctggggctcg ccaagcgccc attcgctgtt gtcccaacca
     2041 agatcgatgt catcggcacg agcgaacggc ttacccagat gcagagctat tgttgcctca
     2101 acgactaccc ctgcctgccg atttccgccg ccacgaacaa agggcttgat gacatgatca
     2161 cctacctcgg gaaacgggtc gatcatttac gaaagacgcc atgcgagaca agctcttagt
     2221 acaagcgaaa cggatcgtca tcaagatcgg cagcagcctg atcgcttcac gcgcggaagg
     2281 cctgcgcccg caacaaatcg agcgattggc cgatgaaatc gcgatgctcc gaacacaggg
     2341 tctcgaaatc ctggtggtct cttccggtgc catcgtctcc ggcatccgaa agttgcagct
     2401 gaaggattac cccaaaagcc tccctgttaa gcaggcagcg gcagcggtag gacagagccg
     2461 gctcatgtgg gcctatgaaa aagcatttga acgtctcaat attcaagcgg cacagattct
     2521 actgacccac caggatctcg ccgatcgccg ccgatttctt aatgctcgtc atacgcttac
     2581 ggccctcatc ggtttcggca tcgtgcccat catcaatgaa aacgataccg ttgcggttga
     2641 tgaaatcagg gtcggcgaca atgacaccct cgcgagcgaa gtggctcacc tggccgatgc
     2701 ggacttgctg gtgatcctct ctgacgtcga tggactgtat acggaagatc cgcgtaaaaa
     2761 cccgtcggcg acattgatcc cgctgatcac ggagattacc caagatatcg aacgcctggc
     2821 aggagtatcc agcacattcg aaggtacagg cggaatggcg accaagcttc gagcggccaa
     2881 gaaagtcggc gagtatggaa tcccgacgtt aatcctcaat ggcgagcggg ccgggctcct
     2941 cccaacggtc atcggcggcg ggcctggtgg aagtctgttt ctcgcccggg agcgtcgact
     3001 gaacagccgt aaacattgga tcgcctttac gcttcgctcc cgaggccagg tccatctcga
     3061 ccagggggct gtggacgctt tgacc
//
LOCUS       DATMTI010000185         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_261895, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000185 DATMTI010000000
VERSION     DATMTI010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261895"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VN039_03105"
     CDS             <1..676
                     /locus_tag="VN039_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HWV45009.1"
                     /translation="MGTTGAGWLVGGWPNKAQAVLGTPRSLCIVRPEQTEGPYFVDER
                     LHRSDIRTDPANRRVTPGAPLTLTFQVMRLHTGECRPIPEAQVDVWQCDAMGVYSDVQ
                     DPGFKTLGQKFLRGHQITDAECNARFLTVYPGWYPIRTVHIHFKIRTTAIAGRNFEFT
                     SQLYFPDELTDRVHTDLPYSSKGRRRVRNQHDFIFRNGGEQLLLNPSATNGSYAATFP
                     IGLTIP"
     gene            982..1344
                     /locus_tag="VN039_03110"
     CDS             982..1344
                     /locus_tag="VN039_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45010.1"
                     /translation="MKTSAIVLFAAVVQLIGSLASANPAMLPKHPGYPMGKAVDPVTG
                     QSLANDPGQANAGGREALNNAALVDDSRSKQDLLFNQNDERLLEKPGAGLLPKVQGPN
                     ITIEPPVKEGTKVSAAPE"
     gene            complement(1456..2529)
                     /locus_tag="VN039_03115"
     CDS             complement(1456..2529)
                     /locus_tag="VN039_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWV45011.1"
                     /translation="MGTLISVASGKGGVGKSVVSANLALALAKGGRQVILADLDVGGA
                     DAHIMFGELNPSVTLTDFLDKRVSRLDEVAIPVIIHPNLRLIAGTGDTLATANMAYAR
                     KKRLMKQFRDLATGIVVIDVGAGTNYHALDFFLMADIHLAVATPEPTSVLDLYRFIKL
                     AAIRRVVACFLARSPLSEVLSNRDFTSVEEVMEIAGAADAGGRDAAAAALQSFRPGLI
                     INRVSDSPQVNVLYLRKILHQYVGGDLTLLGQIPDDPAVSQAVRKFLPVIEAAPESPA
                     AKGLLAVSAAVEQLVSASADRSKEEAKQSEEKQTRVLRSNPACSQDPQASTPLKPDVP
                     PPSVGPRPPGCPNSAQNTSGLVA"
     gene            2765..>4106
                     /gene="pyk"
                     /locus_tag="VN039_03120"
     CDS             2765..>4106
                     /gene="pyk"
                     /locus_tag="VN039_03120"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWV45012.1"
                     /translation="MSMRKAKIVCTIGPASSSPAVLNRLIESGMDAARLNFSHGTHES
                     HAAAITAIRKAAAQCGSRVAIIQDLQGPRIRVGLLPKEGITVNPGQVVRLRTSAQSGE
                     PHGVQASVPAPVPEIPVTYASLTRDLHRGSRVLINDGLIELQSVRIEDDLIECSVIIG
                     GMITSHKGINLPGTQVSAPTLTEKDREDIRFGAEHGVDYVALSFVRGPQDISAARAVL
                     TEHGINTPIIAKIERAEAVAALDEILEQADGVMIARGDLGVEMGPEAVPLLQKKIIVE
                     ANRRRRLVITATQMLESMTQAIRPTRAEASDVANAVFDGSDALMLSAETAVGAHPIEA
                     LQVMDRIIRAAEEGAEPGVVLKRQADLEHLSISESTCAAASFAARATDATAIVAFSES
                     GTTVRLISKQRPSAPILAFTHTEPIKRRMALYWGVRSFIMLRIGETDPHIREAEQ"
BASE COUNT          913 a         1178 c         1144 g          871 t
ORIGIN      
        1 gatggggacc actggtgccg gttggctggt aggtggatgg ccaaacaaag cacaggctgt
       61 tctcggcaca cctcgatctc tctgcattgt ccgaccggaa caaaccgagg gtccgtattt
      121 cgtggacgaa cgattgcatc ggtccgacat tcgtactgac ccggctaata ggcgagtgac
      181 gcctggtgcg ccgttgacgt tgacgtttca agtgatgcgc ctccataccg gggaatgtcg
      241 acccatacca gaggcgcaag tcgatgtttg gcagtgcgat gccatgggag tctactcgga
      301 tgtgcaggat ccagggttca agacgctggg ccagaagttc ttacggggac atcaaataac
      361 cgatgcggag tgcaacgcgc gctttctgac cgtctatccg ggctggtatc cgattagaac
      421 ggtccacatt cattttaaga tccgcacgac agcgatcgcc gggagaaatt ttgagtttac
      481 atctcaattg tactttcccg acgaactgac ggaccgcgtc cataccgatc tcccctactc
      541 atcaaaagga cggcgtcgag tacggaatca gcacgatttc attttccgta acggcggtga
      601 acaattactg ctgaatcctt ccgcaacgaa tggtagctat gcggcgactt ttccaatcgg
      661 tctaacgatt ccttgatctc taccttccgg attccactca ttgactaccc gcatcgaatc
      721 tcgactcccc tcgttccccc catcagctat ctcaagccgc tgttgatttc tgtacagccg
      781 caccctgagc cgattccccc tgtcttgact ctcaacattc tgagagcgat gctaagcgat
      841 gtcccggagg tcttatgaaa ccggggtcga cacttccaca ttttcttacg ttcattgcct
      901 gcccatgccc tgacgctctc cgagggcctt atgcctgcga gggcgttgtt gcctcttcca
      961 ccttagccag gaggtttcgc catgaaaacc tcggccattg tgctgtttgc tgctgtcgtg
     1021 cagctaatag gttccctcgc gtccgctaat ccggccatgt tacccaaaca ccccggctac
     1081 ccaatgggga aagcggttga tcctgtgaca ggccaatcac tggcaaatga tccgggacaa
     1141 gccaatgccg gaggccgaga ggccctcaac aatgcggcac tcgtcgacga cagccgttcc
     1201 aaacaagacc tgctctttaa ccaaaatgac gaacgcctct tggagaaacc tggagcagga
     1261 ctgcttccaa aggtgcaagg tccgaacatc acgattgaac caccggtgaa agaagggaca
     1321 aaggtgagtg ccgcaccgga ataatgaaga ttgtttcgag ctgacggccg gacatccctg
     1381 ataggtaggc ggtgaacgct ggactaacgg agagggtatg acgattacct ctacaccgtt
     1441 cgcaccttca ctgccctagg caacgagccc gctggtattt tgggctgaat tggggcaacc
     1501 aggcggtctc ggtccgactg aaggaggcgg aacatccggt tttagaggag tcgatgcctg
     1561 agggtcttgg gaacatgccg gattggagcg gagcactctt gtctgcttct cttccgattg
     1621 tttggcctcc tctttgcttc tgtcggcaga ggccgagacg agttgctcaa cggcggcaga
     1681 gacggctaac agacctttgg cggcggggga ttccggagca gcttcgataa ccgggagaaa
     1741 cttccgaacc gcctggctca cagctgggtc gtcagggatc tgaccgagca gcgtcaggtc
     1801 cccaccgacg tattgatgga gaatcttgcg aaggtacagg acattaactt gaggactgtc
     1861 ggatacccga ttgatgatta accctggccg aaacgattgc aacgccgccg ctgctgcgtc
     1921 acgcccccct gcgtcggcgg cgcccgcaat ttccatgacc tcctccacac tcgtgaagtc
     1981 tcgatttgag aggacctccg acagggggct tcgcgcaaga aaacaggcta ctacgcggcg
     2041 aatcgccgcc aacttaatga atcgatagag gtccagcacg gacgtcggct caggagtagc
     2101 cacggccaga tgaatgtcgg ccatcaggaa aaagtcgagc gcatggtagt tggtgccggc
     2161 tccgacgtcg atcaccacga tgccggtcgc aagatcccgg aattgtttca tcagccgctt
     2221 cttgcgggca taggccatgt tggcagtcgc caaggtatcg ccggtgccgg caatgagacg
     2281 gaggttcgga tgaatgatga cgggaatagc gacttcgtcc agccggctga cccgtttgtc
     2341 tagaaaatcc gtcaaggtca ccgacggatt caattctccg aacatgatgt gggcatcggc
     2401 tccgcctacg tcgaggtcgg ccagtatgac ctgtcttcca cccttcgcca gtgccaacgc
     2461 aaggttggct gaaaccacgc tcttgcccac tccgcccttg ccggatgcca ctgaaatgag
     2521 cgttcccact gcgtcttcct ttcgttactg aagtcattgt accggctgaa ggacaaaaga
     2581 aaaggaaatg cacgattccg agcggcccaa tcgccattcg cttacaggtg aacaccggtt
     2641 caagatacac tcaagtcaca acgcctctct cgacacaaat ccgtcgtcta tgaggtatcc
     2701 gatgaatgaa tctatgatat tctctttcct ctcttccaac tgaccagcgt ggaggcgctt
     2761 ccacatgagc atgcgaaaag ccaaaatcgt ctgcaccatc gggccggcga gtagttcgcc
     2821 ggccgtgctc aaccgattga ttgagagcgg catggatgcc gctcggctga acttttctca
     2881 cggcactcat gagtctcatg cggcagccat caccgctatc cgaaaggcag ccgcacagtg
     2941 tggatcaagg gtggcgatta tccaggattt gcaaggcccc agaattcgtg tgggtctgct
     3001 gccgaaggaa gggatcacag tgaaccccgg ccaggtggtg cggctacgaa cttcggctca
     3061 gtcgggtgaa ccgcatggcg tccaagcctc cgttccagcc cctgtccctg agatcccggt
     3121 cacctatgct tcactcaccc gcgatctcca tagaggatca agagtgctca tcaacgatgg
     3181 actcatcgag ctacaatccg ttcgcattga agacgatctg atcgaatgtt ccgtcataat
     3241 cggcggcatg atcacctcgc acaagggcat taatttgccg ggcactcaag tcagtgctcc
     3301 tactctgacc gagaaggatc gtgaggacat tcgattcgga gccgagcacg gtgtggatta
     3361 tgtagcactc tcctttgtac gaggccctca ggatatcagc gcggcgcggg ctgtgttgac
     3421 ggagcatgga atcaacacgc cgatcatcgc gaagatcgaa cgggccgaag ccgtggctgc
     3481 gttggatgag atcttggagc aggcggacgg tgtgatgatc gcccgaggag atctgggtgt
     3541 agaaatggga ccggaagccg tgccgcttct gcagaagaag atcatcgtag aagccaatcg
     3601 ccgccgccgc ctggtcatca ccgctactca gatgctggaa tcgatgacac aggccatccg
     3661 tccgacgaga gcagaagcgt cggacgtggc caacgccgtc ttcgacggca gtgacgccct
     3721 gatgctctcg gccgaaacag ccgtcggggc tcatccgatc gaagcgcttc aagtcatgga
     3781 tcggattatt cgcgcggcgg aagaaggggc cgagccgggg gtcgtactca aacgacaagc
     3841 cgacttggaa catctttcga tctcggaatc cacctgtgcc gccgcctctt ttgcggcgag
     3901 ggcaaccgat gcgaccgcaa tcgtggcgtt cagcgaaagc gggactacgg ttcggctgat
     3961 ttccaagcaa cgaccgagcg ccccgatcct ggcattcact cataccgagc ccattaaacg
     4021 gcgcatggcg ctttattggg gagtccgttc gttcatcatg ttgcgtatcg gggagaccga
     4081 tccgcacatc cgtgaggccg aacaac
//
LOCUS       DATMTI010000186         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_135045, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000186 DATMTI010000000
VERSION     DATMTI010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135045"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..766)
                     /locus_tag="VN039_03125"
     CDS             complement(2..766)
                     /locus_tag="VN039_03125"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV45013.1"
                     /translation="MSDNHKHRARIFLHRGDLVQARAAWEAAVADDRTAGSQQELSDS
                     LGNLGNACALVNDFPRAEQCYREVLQIQRTERNLTAVAHTLVNLGNLHIASDHPEKAR
                     PYYLEAMDLLRQVQDNRGLGILYNNLALQEARDGQWDQAVASFKQALDHHRMVGNEEG
                     LAVTYSQLGKCYFDQGDLTRAERCLNNASEHYIKLGNEPAEAAVLRLLTKVYETRQDT
                     VSTLRCLARVVALDERYALPELQADSAHLSGLGPSR"
     gene            complement(879..1301)
                     /locus_tag="VN039_03130"
     CDS             complement(879..1301)
                     /locus_tag="VN039_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45014.1"
                     /translation="MRFDPALAAQTAFKESESELGSDRDTAIELEETFSSNAGVTARE
                     AYEGLLAIAQHYPNAHSFHAFCIYITWQQVTEETIAHHFETGLKLSEAYLASHEGKNP
                     RDIEHITELHGSFRAGLGLEEQDDPQVEYKRDTPKGGD"
     gene            1437..3221
                     /locus_tag="VN039_03135"
     CDS             1437..3221
                     /locus_tag="VN039_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HWV45015.1"
                     /translation="MSTEKPIPVTTPESVSTDPVDRVIHYHVRTKHHFNRYARSLGFL
                     DWASQPDPFRRFEGAQLIPLSLLKADEEPLSPSYQAIYEREAVSAQPVNMRMLSRFFE
                     FALALSAWKKAGESEWALRSNPSSGNLHPTEGYVVLPQLKGFDLKPGLYHYAPKEHGL
                     ELRAGFRADLLECLLRSFAQDAFLLGLTSIHWREAWKYGERAFRYCNHDVGHAIGAVR
                     IAAATLGWNMTLLDGVDQDTVAMLLGTDRKEDFGEAESEHPDCLAVVWPNEAVKSKGL
                     EVRGREEVTDGKKGMPLFLDTSIVKKLAGETWHGKANRLSLEHGVHWDIIDEAAKASW
                     KLETDERSVSLPSPQVTPHPLPLTPHAPSSGHIIRRRRSAVAFDGKTSISAATFFHMM
                     QRVMPWAEHPQLQRPMPWDAWPYDPVIHLLIFVHRVEGLTPGLYVLVRDNRKLSVVQH
                     TMNPEMTWTIAPGCPEGLPLYWLLEGDARKMAAQVSCHQDIAGDSAFSFGMLAEFEGV
                     LRERGAWWYPRMFWEAGLLGQVLYLEAEAAGVRATGIGCFFDDPVHEIVGIQNLSIQS
                     LYHFTIGGPVEDRRLQTLPPYHHLQSRS"
     gene            3246..>3427
                     /locus_tag="VN039_03140"
     CDS             3246..>3427
                     /locus_tag="VN039_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45016.1"
                     /translation="MFKIKKKADKPKQIVLYNIVEDYSEFDAPGNVTVCAMTEPEDLP
                     AHAKVLAESYDVPLEL"
BASE COUNT          753 a          884 c          986 g          804 t
ORIGIN      
        1 gttatcgaga tggaccgagc ccggagaggt gggcggagtc ggcttgaagt tcaggcagcg
       61 catagcgttc gtccagcgcc accacacgcg cgaggcatcg cagagtggag acagtatcct
      121 ggcgagtctc gtaaaccttt gtgagaagac ggagtaccgc ggcctcggcc ggttcgttcc
      181 cgagtttgat gtagtgctcc gaggcgttgt tcaaacaacg ctcggctctg gtgaggtctc
      241 cctgatcgaa atagcatttg ccgagttggc tataggtgac ggcgagtcct tcttcgttac
      301 cgaccatccg atgatgatcg agcgcttgtt tgaatgacgc gacggcctga tcccattgac
      361 cgtcacgggc ctcttggagc gcaaggttgt tgtagagaat gccgagtcca cgattgtctt
      421 gtacctggcg cagtagatcc atggcctcga gatagtaggg acgggctttc tccggatggt
      481 cggaggcgat atgcaggttg ccgaggttga ccaaggtgtg agcgacggcg gtgagattgc
      541 gctcggtccg ttgaatctgg agaacctctc gatagcactg ttccgcacgc gggaaatcgt
      601 tcacgagcgc acaggcgttg ccgaggttcc caagagaatc cgacagctct tgctgactgc
      661 cggcagtccg atcatcggcc acagccgctt cccatgcagc gcgagcctgg accagatcgc
      721 cgcgatggag aaaaatccgt gcgcgatgtt tatggttatc cgacattttc cccgtgaagc
      781 gtgaggcgtg aagcgtgaag ggtggggagg gaaataaccc aagggttctg tctgtctcgt
      841 tcgcctcacc ccttgcgcct tacccctcac ggatttcgtc agtctccgcc tttgggcgtg
      901 tctcgcttat attcaacctg cggatcatct tgttcctcca gccctagacc agctcggaag
      961 gagccatgca attccgtgat gtgctcgatg tcccgaggat tcttcccttc gtgagaggcc
     1021 agataggcct cagacaattt caagcctgtt tcgaaatgat gcgcgatcgt ctcctccgtg
     1081 acctgctgcc aagtgatgta aatacaaaag gcatggaacg aatgagcgtt gggatagtgc
     1141 tgcgcgatgg ctaacaggcc ttcataggct tcgcgcgctg tcacaccggc attggaggaa
     1201 aacgtttcct ccagctcaat agctgtgtcc cggtcggaac cgagttcgga ctcagactct
     1261 ttgaacgctg tttgtgcggc caaggctgga tcaaaccgca tagaacctcg tatctcatac
     1321 agcaagtatt acaagtaccc tagaggatac tggggtaaag gaaaggaggt caatgtgtct
     1381 tgctcagacc atagatttag attgtggagc tctgtagact gcacttattt cttagaatga
     1441 gtactgaaaa accaattccc gttacaacgc ctgagtcagt ttcgacagat ccagtcgatc
     1501 gcgtcatcca ctatcatgtc aggaccaaac accatttcaa ccgttacgcc cgttcgttgg
     1561 gatttcttga ttgggcgagc cagcctgatc cgttcaggcg gtttgaggga gctcagctta
     1621 tcccactttc attactcaag gctgatgaag agcccttgtc gccttcctat caagcgattt
     1681 atgaacgaga agccgtctct gctcagcccg tgaacatgcg aatgctctct cgatttttcg
     1741 aattcgccct ggcgctgtcg gcttggaaga aggcaggtga atccgaatgg gcgctacgaa
     1801 gcaatccatc gtccggcaat ttgcatccca cggaaggcta tgtcgtcttg cctcagctga
     1861 aggggttcga tctgaagccg gggctctatc actatgcacc gaaagaacat ggactcgagt
     1921 tacgggcagg gtttcgtgct gacttacttg aatgtctact gaggtctttt gctcaagatg
     1981 ccttcctctt gggactcacg tcgattcatt ggcgcgaagc ctggaagtat ggcgagcgag
     2041 cattccgcta ttgcaatcac gatgtcggac atgcgatcgg ggcagtacga atcgctgctg
     2101 ccacgctggg ctggaatatg acgctgctgg acggtgtgga tcaagacacg gtggcgatgc
     2161 tactcgggac agatcgcaag gaagactttg gagaggcgga atcggagcat cctgactgcc
     2221 tggccgtggt ctggcctaat gaagcagtaa agagcaaggg attagaggta aggggccgtg
     2281 aagaggtaac ggacggaaag aaggggatgc cgttgtttct cgatacgtcg atcgtgaaaa
     2341 aactggccgg cgaaacatgg catggaaagg ccaatcggtt gagtctcgag catggcgtcc
     2401 attgggacat cattgatgag gcggcaaaag catcttggaa gcttgaaacc gatgaacgtt
     2461 cagtttcgct tccgtctccg caagtaactc ctcacccctt acccctcacg cctcacgcac
     2521 cttcgtccgg ccacatcatt cgccggcgtc gaagcgccgt cgcatttgac ggcaagacat
     2581 caatttccgc agccacgttt tttcacatga tgcaacgggt catgccgtgg gctgaacatc
     2641 cgcagttgca acggccgatg ccgtgggatg cctggcccta tgatccggtc attcatctgt
     2701 tgatatttgt tcatcgtgta gaggggctta caccggggct gtatgttctt gtacgagata
     2761 acagaaagtt gtctgtcgtt cagcacacca tgaatccgga aatgacctgg accatcgcgc
     2821 ccggctgtcc cgagggcctg cctctgtatt ggttattgga aggcgatgcc agaaaaatgg
     2881 ccgctcaggt gagttgccat caagacattg ccggtgacag tgccttctcg ttcggcatgt
     2941 tagccgagtt cgagggggtc ttgcgggaac gaggcgcctg gtggtatccg cgcatgtttt
     3001 gggaagcagg attgctcggc caggtcttgt atctggaagc ggaagcagca ggagtgcggg
     3061 cgacgggaat cggctgcttc ttcgatgacc ccgtccacga gatcgtcggc attcagaact
     3121 tatccattca atccctctat cattttacga ttggcgggcc ggtggaagat cgacgcctgc
     3181 agaccttgcc gccgtaccat catctgcaat ctcgttcata agcctcgatg gctgtgaggt
     3241 agctcatgtt caaaatcaag aagaaggccg acaagccgaa gcagatagtg ctctataaca
     3301 tcgttgaaga ctattcggag ttcgacgccc cgggaaatgt aaccgtatgt gcaatgactg
     3361 agccggaaga tctcccggca cacgccaagg tccttgcaga aagttacgat gtgccgcttg
     3421 agttgat
//
LOCUS       DATMTI010000187         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_135229, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000187 DATMTI010000000
VERSION     DATMTI010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135229"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..778
                     /locus_tag="VN039_03145"
     CDS             8..778
                     /locus_tag="VN039_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HWV45017.1"
                     /translation="MKRPITVTRRMEAGGPFYDLVCGQGRLEAFQELGQEYVPALVVS
                     ANKEDCLIASIVENCARRQHRAIDYLHDIGAMKGRGHSIPEIARLTGLTPEYVHAVVR
                     LMEKGEERLLRSVETGQIPFSVAVQIAEAEEHEVQAALQNAYENKLLRGKKLMTAKRI
                     VEQRRRRGKGLKFSIKSRSQPLSSEALVKAFQEDVDRKRMLIRRAEAAKARLTFITEA
                     LRKLLADTQFTGILSEEHLVTLPEALASKLEHQEGTFS"
     gene            775..>1548
                     /locus_tag="VN039_03150"
     CDS             775..>1548
                     /locus_tag="VN039_03150"
                     /inference="COORDINATES: protein motif:HMM:NF019147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning protein RepB C-terminal
                     domain-containing protein"
                     /protein_id="HWV45018.1"
                     /translation="MSPTSRKPLVSLSFENTSLRIPIDEIMPLKQLSASTLKSVKYAQ
                     IAASIAELGIIEPPVVIRDREVPDKFHLLDGHIRVDVLARNGETEVVCLVGTEDEAYT
                     YNRRVSRLATIQEHKMILKAIEKGVPEERLARVLDVNISSIRHKKTLIGGLCDEVVDM
                     LKEKHVPLNTIGELKKMKSMRQLEAAQLMIAMNKFTVTYAKSLVASTPDNMLLNPKKK
                     IGGLSEQQIELMEREAATLDREFKTIEQDYGADHLDLVLA"
BASE COUNT          416 a          398 c          415 g          319 t
ORIGIN      
        1 gatcggtctg aaacggccga ttacagtgac ccgtcgtatg gaggccggcg gcccgtttta
       61 cgaccttgtg tgtggtcagg gacgcctgga agccttccag gaactcggcc aggaatacgt
      121 tccggcgctc gtcgtcagcg cgaacaaaga ggactgcctg attgccagca ttgtggagaa
      181 ctgcgctcgg cgtcagcatc gtgctatcga ttatctccat gacattggcg cgatgaaagg
      241 acgaggtcac agcatacctg agattgcccg cctgacaggt ctgacgccgg aatacgtcca
      301 cgccgttgtt cggctcatgg aaaaaggtga ggagcgactg ctgcggtcgg tcgagactgg
      361 ccagataccc ttcagtgtcg ctgttcaaat tgccgaagcc gaggaacacg aagtacaggc
      421 cgccctccaa aacgcgtacg aaaacaaatt gctacgcggt aaaaaattga tgaccgcgaa
      481 gcgcattgtc gaacaacgcc gccgacgtgg caaaggtctg aagtttagta tcaaatcaag
      541 gtctcaaccg ctgtcgtccg aagcgctggt taaagctttt caggaagatg ttgatcgcaa
      601 gcggatgctg atccggagag ctgaggccgc caaggcgcgc cttacgttca taacggaagc
      661 cttgagaaaa ctcctcgccg acacacagtt caccggcata ctgtcggaag aacacctggt
      721 cacccttccg gaagccctgg cttcgaaact ggaacaccaa gaaggaacgt tctcgtgagc
      781 cccacctctc gaaaacctct cgtatcacta tcgttcgaga acacgagcct cagaattcct
      841 atcgatgaaa ttatgcctct caagcagctc agcgcatcaa ctctgaaatc ggtcaaatat
      901 gcacaaatcg cagcctcgat tgccgagttg ggcatcattg agcctccggt cgtaattcgg
      961 gacagggagg ttcctgacaa gtttcatctt cttgacggtc atatccgggt agatgtcctg
     1021 gccagaaatg gcgaaacaga agtggtgtgc ttggtaggta ccgaagacga ggcctacaca
     1081 tacaatcgcc gcgtcagccg cctggcgacc atccaggaac acaagatgat tttgaaagcg
     1141 atcgagaaag gtgttcccga ggagcgtttg gcacgagtac tggacgttaa tatttccagt
     1201 atccgccaca agaaaactct gattggtggg ttatgcgacg aagtcgtaga tatgttgaag
     1261 gaaaagcacg tcccgttaaa cacgattggt gaactcaaaa agatgaagtc gatgcgtcag
     1321 ctcgaggccg cccaattgat gatcgcgatg aacaaattca cggtaacgta tgcaaagtca
     1381 cttgtggcct cgacgccaga caacatgctt ttaaatccga agaaaaaaat tggcggatta
     1441 agcgaacaac aaattgagct tatggaacgc gaggccgcga cgcttgatcg ggaattcaaa
     1501 accatagaac aggactacgg agccgatcac ctagaccttg ttctcgct
//
LOCUS       DATMTI010000188         1820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_347406, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000188 DATMTI010000000
VERSION     DATMTI010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347406"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VN039_03155"
     CDS             complement(<1..1006)
                     /locus_tag="VN039_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HWV45019.1"
                     /translation="MYTRLRVRGPIIGKQEAYYAGEQRLTFATDEWLRANGSRYASFS
                     DNWCASVANAETERVRVTGIKLRGDRDSAVKRASKLWDQWSLNELEAQSSQGFLTSFV
                     AKRSYVLVWGDGENAQVTWEHPANVEIEYDWMNPRQRKAAMKTWVDESAEYLTLYTAD
                     AVYKWQRPRGMVQTDGRPQSEQMEVTPYSAVGQWTPRQPVGDDSWPLPNPLGAVPIVE
                     IPNRPLLRGEPLSEIDPVIPKQDAINLLWAYLMFAADYASMPARVLLGATPPMRKILD
                     DQGNVVGQEPLTMRELNETRFAVFSGENAKIDQWDPAKLDVFTETIDVLVGHIASQTR
                     T"
     gene            complement(1058..>1820)
                     /locus_tag="VN039_03160"
     CDS             complement(1058..>1820)
                     /locus_tag="VN039_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45020.1"
                     /translation="SVDAIERDAEELIETSPADAERFFGNRIVAGSGAWMDMAKWESK
                     KVSPVVVSPKTKVCLGFDGSDNNDFTGIRLETLGQYQFTPTYGDRELKTLWRPADHGG
                     RIPRAEVNAAVDELANRFEIVRAYCDPFLWESEVDEWAAKYGEKVFVKWPTNRVMQMH
                     ASLERFRTDVYNPESLFRHDSDDLVTAHVRNASVRSRPLNPLTKERQYILGKPAGADH
                     QKIDFAMSSVLAHDAVMDAIADGANQDTTNYAWIM"
BASE COUNT          424 a          637 c          420 g          339 t
ORIGIN      
        1 gcgtccgcgt ctgcgaagca atatgcccaa ccagaacgtc aatcgtctcc gtgaacacat
       61 ccagtttcgc cggatcccac tggtcaatct tcgcgttttc acccgagaac accgcaaacc
      121 gggtctcgtt cagctcgcgc atcgtcaacg gttcctgacc aacaacattg ccctggtcat
      181 ccaggatctt ccgcataggc ggcgtcgcac caagaagaac ccgcgccggc atagacgcat
      241 aatccgctgc gaacatcaga tacgcccaca acaggttgat cgcatcctgc ttcggaatca
      301 ccgggtcaat ctccgacaac ggttcaccgc ggagcagcgg acgattcggg atctccacaa
      361 tcggaacagc acccaacggg ttcggcaacg gccacgaatc atcaccaacc ggctgacgcg
      421 gagtccactg accaaccgca gaatacgggg ttacctccat ctgttccgac tgtggcctac
      481 cgtccgtctg caccatgccg cgcgggcgct gccacttgta caccgcatcc gccgtgtaca
      541 aagtaaggta ctcagcggac tcatcaaccc acgtcttcat cgcggcctta cgctgccgag
      601 ggttcatcca gtcgtactcg atttcgacgt tcgccgggtg ctcccacgtg acctgagcat
      661 tctccccatc gccccacaca agaacgtagg aacgcttcgc cacgaacgaa gtcaggaacc
      721 cctgcgacga ctgcgcctca agctcattca acgaccactg atcccacagc ttcgaagcac
      781 gcttcaccgc agaatcacga tcaccacgca acttgatgcc cgtcacccgg acgcgctcag
      841 tctccgcatt cgccacagac gcgcaccaat tatccgagaa cgaggcgtag cgggaaccgt
      901 tcgcacgcaa ccactcatca gtggcaaacg tcaaacgctg ctcacccgcg taatacgcct
      961 cctgcttccc aataatcggg ccgcggacgc ggagacgcgt gtacattcgg ttagccaggt
     1021 ccagggcctc tctagccttc acatcggccc cttccggcta cataatccat gcgtaattcg
     1081 ttgtgtcctg gtttgcgcca tccgcgatag catccataac cgcgtcatgc gccaaaacag
     1141 acgacatggc aaagtcgatc ttctgatgat ccgcaccagc aggtttcccc aagatgtact
     1201 gacgctcttt cgtcaacgga ttcaacggcc gcgaccgcac cgacgcgttc cgaacatggg
     1261 cggtcaccag atcgtcggaa tcgtgacgga aaagcgactc tgggttgtac acgtccgtgc
     1321 ggaaccgctc caacgaggca tgcatctgca tcacccggtt agtcggccac ttcacaaaca
     1381 ccttctcgcc atacttcgcc gcccactcat ccacctcgga ctcccaaagg aacgggtcac
     1441 aataggcgcg cacaatctca aaccggttcg ctaactcgtc aacagcagcg ttcacctcag
     1501 ctcgaggaat ccgcccaccg tgatccgccg gccgccacaa cgtcttcaac tcgcggtcac
     1561 catacgtggg tgtgaactgg tactgaccta acgtctctaa ccggataccc gtgaagtcgt
     1621 tattgtccga accgtcaaat cccagacaca ccttcgtctt gggcgacaca acaaccgggg
     1681 aaaccttctt cgactcccac ttagccatgt ccatccacgc accagaacca gccacgatac
     1741 ggttaccgaa gaaccgttcc gcatcagccg gggaagtctc aataagctcc tcagcgtcac
     1801 gctcaatagc gtcaaccgac
//
LOCUS       DATMTI010000189         1862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_135320, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000189 DATMTI010000000
VERSION     DATMTI010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135320"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..422
                     /locus_tag="VN039_03165"
     CDS             93..422
                     /locus_tag="VN039_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45021.1"
                     /translation="MAVEPKRDIMMIGSASAPILYFDNAPLYGVMGECVEVDLTSRVL
                     NATEGNEVFSEAVCVGHLKCGVSAAQSLIIALSKALKLMGKEVPTEKPQSAFSDYVEG
                     EMPDLPN"
     gene            600..1676
                     /locus_tag="VN039_03170"
     CDS             600..1676
                     /locus_tag="VN039_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45022.1"
                     /translation="MPSDIDLVKEAIIETEKEIFGDAFGKEDMTLDASGDRSLEEMGA
                     GLEGQHEPDEDDGEADADGENPEVDAKTEGESGKSDEAEGKGKQPDPEANADDGKGKD
                     GRVPSGRVREANERARAAEAERDALKQQFEAQKAESQKAIDALNARFDALMRAQQQTQ
                     QPRVEPAKTEPETPPDIFEDPKGFAEYLQKGPKSALEQMERRFEEMRINNSMSMARVK
                     HGETFDAAFQAFVEAGKSGDPEARVLGQRILKSSDPGEGVVQWHRRNETLRRVGSDPD
                     AYEAKIKEDATKALMQDPNFRKQVIDELRAEAETGDNGRPRTTVKLPPSLNRAAGNNR
                     SMAGDRSAFDGSDSAIFDSAFNST"
BASE COUNT          482 a          493 c          538 g          349 t
ORIGIN      
        1 atcaactata acggtccgct ctccgcattc aaggctatcg atgcaaccag tagcactggc
       61 acgctcgacg tgtcttatta tgagtgatgc ccatggctgt tgaaccgaag cgcgatatca
      121 tgatgatcgg ctctgcctca gcgccgatcc tctattttga caatgcgccg ctctacggag
      181 tgatgggtga atgtgtcgag gttgatctga cctcgcgcgt cctcaatgcg accgagggca
      241 atgaggtttt tagcgaggcg gtttgcgtcg gacatctgaa atgtggcgtt tcggctgcgc
      301 aatcccttat catcgcgtta tcgaaggctt tgaagttgat gggcaaagaa gtcccaaccg
      361 agaaaccgca atcagcattt tcggattatg tcgaaggtga gatgcccgac cttccgaact
      421 gagtttcgtt ggcgtcacga tacgacgcac ggccacggat agccgactga atatccgccg
      481 caggcgaccg cgacaaggcg cgatgccaaa cccggcactg gcatgccccg accatctcac
      541 gatggctcgg gcgaattgtg ctgccgcaat cacagcgaca tcgtgaaaag ggttggcgaa
      601 tgccaagtga catcgatctt gtaaaggaag ccatcatcga aaccgaaaag gaaatcttcg
      661 gtgatgcttt cgggaaagaa gatatgacgc tggacgcatc cggcgatcgt tcccttgaag
      721 aaatgggggc gggactggaa ggccagcacg agccggacga agacgacgga gaagcagacg
      781 ctgacggaga aaatcctgag gtcgatgcaa aaaccgaagg cgaatccgga aaatcggacg
      841 aagcggaagg taagggcaag cagcctgatc ccgaagctaa tgccgatgat ggaaagggta
      901 aagatgggcg tgttccgtcc ggacgggtca gagaagccaa tgagcgagcg cgcgctgcgg
      961 aagcggaacg cgatgcgctg aaacagcagt tcgaagccca gaaggcggaa agtcagaagg
     1021 cgatcgatgc gctcaatgcg cgtttcgatg ctctgatgcg ggcgcagcag cagacacagc
     1081 aaccgcgtgt cgagcctgcc aaaaccgaac ccgaaacccc tcccgatatc ttcgaagatc
     1141 ccaaaggttt tgcggaatac ctccagaaag gcccgaaatc tgctcttgag cagatggagc
     1201 gtcgtttcga ggaaatgcgc atcaacaaca gcatgagcat ggcccgcgtc aagcacggag
     1261 aaacgttcga cgcggcgttt caggcgtttg ttgaagcggg gaaaagcggt gatcctgaag
     1321 cccgcgtcct gggccagcgc atcctgaaat cgtccgatcc gggcgagggt gtcgttcaat
     1381 ggcacagacg caatgagacg ctgcgtcgcg ttggttctga tcctgatgcc tatgaggcga
     1441 agatcaagga agacgcaacc aaagctctca tgcaggaccc gaatttccgc aagcaagtga
     1501 tcgatgaact ccgcgccgaa gccgagactg gcgacaacgg gcgcccgcga accacagtca
     1561 aattaccgcc ctctctaaac agagcggctg gaaacaatcg aagcatggca ggcgatcgat
     1621 ccgccttcga cggttctgac agcgccatct tcgattcggc tttcaattcg acatgacgcg
     1681 atgagcttcc gcattcgtgc ggattgcgga cgcaccatgc cctgacaaag gatcacggca
     1741 atggccgtta ctacggtagc agacaacaac aaacttattg tcttccgaaa ggaagtcttc
     1801 aaagaatacg ttcgcgaaaa cctgttctct ccctatatgg gatcggacat taacgccatc
     1861 at
//
LOCUS       DATMTI010000190         4743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_135343, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000190 DATMTI010000000
VERSION     DATMTI010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135343"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VN039_03175"
     CDS             <1..357
                     /locus_tag="VN039_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45023.1"
                     /translation="ARDDGSDTLHKEAFMKEVIKGLTVSTSVIPAPGGPRRLSPNHLL
                     ELVTRLCIFKAERWGRRALFYERHRDYFPRLSAGRFVNRCRELEARYRALAQIMFLIP
                     AEDRLVPIRALTLSKL"
     gene            610..2349
                     /locus_tag="VN039_03180"
     CDS             610..2349
                     /locus_tag="VN039_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWV45024.1"
                     /translation="MKQGPSSESALPTITGFSPGFVALGLEAPLLATLETLGYEEPTP
                     IQREAIPPLLAGRDLLGQAATGTGKTAAFALPMLQLISHAARVRPSALVLVPTRELAI
                     QVGEAVQRYGKELRVSMLPVYGGQAIGPQLHALKRGVDVVVATPGRALDHIRRGTLKL
                     QELRIVVLDEADEMLDMGFAEDLDAILAGTPKIKQTALFSATIPPRIRAIAHRHLQNP
                     IEITIAKEPVKAGTAPRVQQTAYIVARPHRAAALARVIDVAGANSALVFCRTRLEVDE
                     VTAMLTGRGHRAEAIHGGMSQGQRDRVMQTFKAGQTELLVATDVAARGLDIPQVSHVI
                     NYDLPSSAEIYIHRIGRTGRAGREGAAITILDPREQRLLRTIEQHTKARVTVVPIPSV
                     ADLRSRQLDRVQAAVEQVLRDGSLDTFKHLVERLSAHPPAEVAAAALKLLVRAQGGDR
                     QEQEIPLIPARQPEFGRPIRDARHSGGRLHGGSAGAERPPTGRSRHGGRTVEMARVYV
                     GVGREAGIRPGDLVGAIANEARLNSNIIGAIEIEQRFSIVDVPASMASGIIEVLGRAR
                     IKGRRVPVRLFRD"
     gene            2577..4616
                     /locus_tag="VN039_03185"
     CDS             2577..4616
                     /locus_tag="VN039_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HWV45025.1"
                     /translation="MKNSQPLVPATVTLPEITVKAVVLSVILAAVLAAANAYLGLFAG
                     MTVSASIPAAVASMAILRLFRESNVLENNLVQTAASSGEALAAGVIFTIPALLLVGYW
                     SDFDYWQTVLIATVGGLLGVLFTIPLRRALIVTAALRFPEGLATAEVLKVGAAERSEA
                     GSRTSTKNFQSLLSAAFLGGLVKFGESGLRLWTDSLEGAAHVGRTVFYAGLNLSPALL
                     AVGFIIGLQTAAVVFLGGVIGWVILMPAYGLVQGLPPDRTGPAAAMSIWSGQIRYVGI
                     GAMLTGGLWTLTKLREPVWKSLQTLRASYRGVQVKGTAQVEIKEHLKGENVPRTEQDA
                     SFLWIVVPFGLSLLPMAWIYSTVVNSLLLGLLMTIVMALAAFLFSSVAAYMAGMVGSS
                     SNPVSGVAIATIMIAALLLVLFMGAGHPAGPAAALVIGAVVCCAAAMGGDNLQDLKTG
                     QLVGATPWKQQVMQVVGVVTGALVLVPVLSLLQAKYGIGEPTSSHPHPLSAPQAMLMA
                     NLTRSVFGAGLPWHLVGLGAVIGILIILADRRQERRGSALRLPVLAVALGIYLPLKLS
                     ATIFVGGLIAALANRRLGGRQESSQGGLLFAAGLITGEALMGIMLAMPIAFAALWPGF
                     STDPFMIFAVPPLGGWPGLAMVAVVGFALYRIAVERGQNALTSTTEAASQDTSSE"
BASE COUNT          907 a         1359 c         1453 g         1024 t
ORIGIN      
        1 gctcgagacg atggctcaga tacattgcac aaggaggcct ttatgaagga agtaataaag
       61 ggactgaccg tatccacttc tgtgatccct gctccaggag gaccccggcg gttgtcgccg
      121 aaccatcttt tagaattggt cacgcggttg tgtatcttca aggccgagcg ttggggacgc
      181 cgagcgctct tttacgagcg gcaccgcgac tatttccccc ggttgtccgc tggtcgattc
      241 gtgaatcgat gccgtgagtt ggaagcgagg tatcgagcgc tcgcacagat catgttcctt
      301 atcccggcag aggaccggtt ggttccgata cgcgcattga ccctgtcgaa gctgtagggc
      361 aacagggagc gattctcgta ggtaccgtga tccctgttcg atggatcgag aatcgctctc
      421 tcctctcgta ctcggttcat accactgatg ctcgctgatc cgcctcacga tggtcacctg
      481 atgaccgtct tcgatccgat cttgcgctac cgccacatta caacgtaaca tcaagccttc
      541 tgtgagtcgt cgtcctacat tctccacgat tccaccacca tcgacggcag aaaggtgctg
      601 agtatcatta tgaaacaggg cccgtcctca gagtccgctc ttccgaccat cacggggttt
      661 tcaccaggct tcgtcgcctt gggacttgaa gcgccgctgc tcgcgacgtt ggaaacgctg
      721 ggctatgagg aacccacccc catacaacgc gaagcgattc ccccgctcct cgcaggccga
      781 gatctgcttg gacaggcggc gaccggcacc ggcaagaccg ccgccttcgc gttgccgatg
      841 ctgcagctga tcagtcatgc agcgagggta cgcccgtctg cccttgtgct cgtcccgacc
      901 cgcgagttgg cgattcaagt cggagaagcg gtgcagcggt acggaaagga actgcgagtg
      961 agcatgttac ccgtgtacgg tggacaagcg atcggtccgc aattgcacgc gctgaaacgg
     1021 ggagtcgacg tggtcgtcgc cacaccgggc cgcgcactgg atcatattcg gcgcggcacg
     1081 ctgaaactac aagaattgag gatcgtcgta ttggacgaag cggacgaaat gctcgacatg
     1141 ggatttgcgg aggacctgga cgcgatcctg gccgggacgc ccaagatcaa acagaccgcc
     1201 ttgttttcag cgaccatccc tccaagaatc cgcgcgatcg cccatcggca tcttcagaac
     1261 ccgatcgaga tcaccattgc caaagaaccg gtcaaggccg gcacggcccc acgtgtccag
     1321 cagacggcgt atatcgttgc gaggccgcat cgggcggcgg cgctcgccag agtgatcgat
     1381 gtggcaggcg ccaactccgc gctggtcttc tgcagaacca gactggaagt cgatgaagtg
     1441 acggcgatgc tgacgggccg tggccatcga gccgaggcca ttcacggcgg catgagccag
     1501 gggcagcgcg accgtgtcat gcagacgttc aaggccgggc aaaccgaatt actggtggcg
     1561 accgatgtgg cggcccgggg attggatata cctcaagtgt cacacgtgat caactacgac
     1621 ctgccgtcct ccgctgaaat ctatatacat cgaatcggcc ggaccggtcg ggccggtcgg
     1681 gagggtgcgg cgatcaccat cctcgaccct cgggaacaac gcttactacg gaccatcgaa
     1741 cagcacacga aagctcgggt caccgttgtg ccgatcccga gcgtggccga tctccgctcc
     1801 agacagttgg atcgcgtgca agccgcggtg gaacaggtat tacgcgatgg atcgttggat
     1861 acgttcaagc acctcgttga gaggctttcc gcccatccac cggcggaggt ggcggcggcg
     1921 gcgttgaagc tgctggttcg cgcgcaaggc ggcgatcgcc aggaacagga gattcctctc
     1981 atacctgcca ggcaaccgga gtttggacga ccgattcggg acgcacggca ttcgggtggt
     2041 cggcttcacg gtggatcggc tggagcagaa cgtccgccga caggacggtc gcggcatggc
     2101 gggcgaacgg tcgagatggc acgcgtgtat gtcggggtgg ggcgtgaggc aggcatcaga
     2161 cccggcgatt tggtgggcgc gattgcgaat gaagcgaggc ttaactccaa catcatcgga
     2221 gccatcgaaa tcgaacagcg gttttccatc gttgatgtac cggcctccat ggcctcgggc
     2281 atcatcgaag tactcggccg cgctcgcatc aaaggccgca gagtgcccgt acgactgttt
     2341 cgtgactagc ttgtgcgatc acaaaaggcc gactatcatg gttgggagcc tgtctgacat
     2401 tgacctttct actgcgacga acgatctgca gcgatctgcg ttgttctcgg ccaggaaaaa
     2461 tcctcgatgt attccagtga atacacctcc agtttttccg ggcctgcggc ctcgcatctt
     2521 gccgcagctc cttcgcctcg tcacgaacgg caatgtcaga ccggctccta gctatcatga
     2581 aaaattccca gcctctcgtc cctgccaccg tcacactccc tgagattacc gtcaaagcgg
     2641 tcgtgctgtc ggtcatcctc gcagcggtct tggcggctgc caatgcctac ctcggcttgt
     2701 ttgccggtat gacggtatcc gcgtctattc ctgcggcagt cgcttccatg gcgattttgc
     2761 ggttgtttcg tgagtcgaat gttctcgaaa acaacctcgt tcaaaccgcc gcttcatcgg
     2821 gcgaagcgtt ggcggccggc gtcatcttca cgattcctgc actcctactg gtcgggtatt
     2881 ggtccgactt cgactactgg cagacggttc ttattgccac agtcggcgga ttactcggtg
     2941 tgctgttcac catccctctg cgacgggcgt tgatcgtaac ggcggctttg cggttcccag
     3001 agggattagc cacggcggaa gttctcaagg tcggcgctgc ggaacggagt gaagccggtt
     3061 cacgcacatc aacaaagaat tttcagtccc tgttgtcggc tgcttttctc ggcggcttgg
     3121 tgaagttcgg ggagagcggc ctgcgcctgt ggaccgactc gctggaaggg gctgctcacg
     3181 ttggacgcac cgtgttctac gccggactca atctttcgcc ggcgcttctt gcagtcggat
     3241 tcattatcgg tctacagaca gccgcggtgg tctttcttgg aggggtcatc ggatgggtga
     3301 tcctgatgcc tgcatatgga ctcgtccaag ggctcccacc tgatcggaca ggaccggcgg
     3361 cggcgatgtc gatctggagc gggcagattc gatacgtggg catcggagcg atgctgaccg
     3421 gcgggctgtg gacgctcacg aaattgcgtg agccggtctg gaagagtctt cagacgctcc
     3481 gggcaagtta tcggggagtc caggttaagg gtacggctca ggttgagatt aaggaacatc
     3541 tgaagggcga aaacgtaccc aggacggaac aggatgcttc attcctgtgg atcgtcgtgc
     3601 catttggcct gtcgctcctg ccgatggcct ggatctattc gacggtcgtg aacagtctcc
     3661 ttctcggtct gctgatgacc atcgtgatgg cacttgccgc cttcctcttc tcgtcggtcg
     3721 ccgcgtatat ggcggggatg gtcggaagtt caagcaaccc cgtctcaggc gtggccatcg
     3781 ccactattat gatcgctgca ctgctgctcg tacttttcat gggagccgga catccggcgg
     3841 gaccggcggc cgcattggtc atcggagcag tcgtctgttg cgccgccgcg atgggcggag
     3901 acaatctcca agatctcaaa acaggtcagc tggttggggc gacgccatgg aaacaacaag
     3961 tcatgcaagt cgtcggagtg gtgacgggcg ctctggtcct cgtgccggtt ctgtctctcc
     4021 tccaagccaa gtatggaata ggtgagccga cgagcagcca tccgcatcct ctcagtgcgc
     4081 cacaggccat gctgatggcg aacttgacac gaagcgtttt cggagccgga ttgccctggc
     4141 atctcgtagg attgggggct gtgatcggga ttctgatcat tctggcagat cggcgtcaag
     4201 aacgccgtgg cagcgcgttg aggttacccg tgcttgccgt ggcgcttggg atctacttgc
     4261 cgctgaaact ttcggcgacc atttttgtcg gtggactcat tgcggctttg gcgaataggc
     4321 gtttgggtgg aaggcaggag tcgtcgcagg ggggattgct ctttgccgcc ggactgatca
     4381 ccggcgaagc cttgatgggg atcatgctcg cgatgccgat cgcattcgcc gccctgtggc
     4441 ccggcttcag caccgatcca tttatgattt ttgccgttcc tccgttgggg gggtggcccg
     4501 gccttgccat ggtggccgtc gtgggattcg cgctctatcg gatcgcggtg gagcggggcc
     4561 agaacgcgct gacatccacg actgaagcgg cgtcgcagga tacctcatcg gagtgagata
     4621 tcacgatgag tatcacggca agaccggaca cgacttccta aaatcgtcgt cccttggtat
     4681 tctaaatgcc gctgttggtt cgtaacacaa gtcatcacgg aggtgaacat gaagatatcg
     4741 acg
//
LOCUS       DATMTI010000191         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_50063, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000191 DATMTI010000000
VERSION     DATMTI010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50063"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VN039_03190"
     CDS             complement(<1..965)
                     /locus_tag="VN039_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HWV45026.1"
                     /translation="MLHADLKKIYDDGQPNASAIMLPDGELIGRGYFEGRFAPLDGIF
                     VIGHKIQRFPTGSVKTVPAFVTSHRQALGRGTTALLGTLRVAVVGCSGTGSVVIELLS
                     RLGVKELILIDPDDVEEKNLNRIVNSSWVDATMATPKVEALARVITANLGRDAPRIIP
                     VRGSLSEAWRQAATADVVFGCTDSSEARMHLDRLCHHYVMPYIDVGVDLRADENDGIR
                     YAGMAVHYLRPGGDSLLARRGYRMSVGEAEALRRTDPTLYAERVKTGYIEGVEEDRPA
                     VISINTQAASMAVNELLARLHGYRAGNPEEHASRRINLVEEFQMS"
     gene            957..1277
                     /locus_tag="VN039_03195"
     CDS             957..1277
                     /locus_tag="VN039_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45027.1"
                     /translation="MQHPITLIVSSRESIGAGWVTVKMHDGRRLQLLSSLKDRVKTLD
                     TPSYVGGCPSTRYMGNRENLPRAKNAAVGITAAPTQEPRRGLAPVSGMQMDVQLSVLL
                     FCHP"
     gene            complement(1289..>1923)
                     /locus_tag="VN039_03200"
     CDS             complement(1289..>1923)
                     /locus_tag="VN039_03200"
                     /inference="COORDINATES: protein motif:HMM:NF025817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multiubiquitin domain-containing protein"
                     /protein_id="HWV45028.1"
                     /translation="GQRYRFMLDKTPHVSDEAELTGRQILAFGNMDPQQYNLFQAEHG
                     GHRIPVGADQVVDLTKPGIERFFTMKNEHSNGDGPAKPEFDLPADDLAYLRSLDLEWE
                     AFQADGHRWLLIHNWPVPQGLNPDHADIALRIDPNYPMSQIDMAYFRPPLSHALGLNM
                     PNLVAIQIRGESWQQWSRHRPPDGWRPGIDSLESHLAYVDNFLAHAVGGN"
BASE COUNT          405 a          550 c          557 g          411 t
ORIGIN      
        1 ttggacatct ggaattcctc aaccagattg atgcgacgag aagcatgctc ctccgggttt
       61 ccggcacggt atccatgtaa gcgtgcaagc aactcgttca ctgccatgct ggccgcctgc
      121 gtgttgatgg aaatcactgc aggcctgtct tcttcgacgc cctcgatgta gccagtcttc
      181 acgcgttcgg cgtaaagcgt tgggtcggtt cgccgaagcg cttcggcttc accgaccgac
      241 attcgatagc ctcgtcgggc gaggaggctg tcaccgccgg ggcgcaagta gtgaaccgcc
      301 atgccggcgt agcggattcc atcattttca tccgcgcgaa gatcaacgcc gacatcgatg
      361 tacggcatga cgtagtgatg gcagagcctg tccagatgca ttctggcttc ggaactatca
      421 gtgcatccga aaaccacgtc cgctgtggcc gcttggcgcc aagcttcgga caatgatcca
      481 cgcacaggaa taatgcgagg ggcatcgcgg ccgagattgg cagtaatcac tcgcgcgagt
      541 gcctccacct tcggcgtcgc catcgttgca tccacccacg aagaattgac gatgcggttg
      601 aggttcttct cctcgacatc atccgggtcg atcaggataa gttccttgac tcctagacga
      661 gagagaagct caatcaccac gctcccggtg cctgaacatc ctacgaccgc aacgcgtagt
      721 gttccgagca acgccgtggt gccacgtcca agtgcctggc gatgactcgt gacgaaggcc
      781 ggtaccgttt tcacactgcc tgtggggaat cgttgaatct tgtgccctat gacgaagatt
      841 ccatcgagcg gagcaaaccg accttcgaaa tacccgcgtc cgataagctc accgtcgggc
      901 aacatgatcg cactggcgtt tggctgccca tcgtcataga tttttttgag gtccgcgtgc
      961 aacacccgat cacattgatc gtcagttctc gagaatccat tggcgccgga tgggtgactg
     1021 tgaagatgca cgatggccgg cgactccagc tcctgagcag cctcaaggac cgggtaaaga
     1081 ctctcgatac gccaagttat gtgggtgggt gtccgagcac acgctacatg ggcaatcggg
     1141 aaaacttgcc tcgcgcaaag aatgctgcgg tcggcatcac ggcggcgccc acacaagagc
     1201 cacgccgcgg cctcgcgccc gtctccggga tgcagatgga cgtgcaactg tctgtgttgc
     1261 tcttctgtca tccgtagaga aagtcgcatc agttgcctcc caccgcatgg gcaaggaagt
     1321 tatcgacata ggccagatgg gattcgaggg aatcgattcc cggacgccaa ccatccggtg
     1381 gtcgatgacg agaccactgc tgccaggact cgccgcgaat ttgtatcgcg acaaggttgg
     1441 gcatgttcaa tccaagtgcg tggctgagtg gcggacggaa ataggccata tctatctgcg
     1501 acatcggata atttggatcg atgcgcagtg caatgtcagc atggtcagga ttcagacctt
     1561 gcggcacggg ccagttgtga ataagaagcc agcgatgccc gtcggcttgg aaggcttccc
     1621 actccaggtc cagactacgc aggtaggcta gatcgtctgc gggaaggtca aactctggct
     1681 tggcgggtcc gtcaccattg ctgtgctcat tcttcatcgt gaagaatcgc tcgatacccg
     1741 gtttggtcag gtcgacaacc tgatcagcac caaccggtat tcgatgcccg ccgtgttcgg
     1801 cctggaacaa gttgtactgc tgcggatcca tgttgccgaa ggcgaggatc tggcgcccgg
     1861 taagctcggc ttcgtcggac acatggggcg ttttgtcgag catgaagcgg taacgctgcc
     1921 cct
//
LOCUS       DATMTI010000192         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_305392, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000192 DATMTI010000000
VERSION     DATMTI010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305392"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /locus_tag="VN039_03205"
     CDS             complement(<1..1871)
                     /locus_tag="VN039_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV45029.1"
                     /translation="MDVIATHSNADFDGLASVVAARKLFPEAKLVLPGGGQEAVRNFL
                     AVHDLDITKLKDIDLSKVTRLVLVDTQEPDRIGALKSCIENPAVEVVVFDHHLEPHAS
                     CTDRSKQSVIESVGATTTLLIEQLRRRHIPVTPFEATVLALGLYEETGSFTFTSTTSR
                     DFEAGAFLAAAGADLNMVTEILHRPLDPDAVALLNDFLEHSEVHYLEGRKILVATSTV
                     DRCRGEAAGVVHRLAELQGVDAVLVAVMMADRVEVIGRSRRPEIDVGWIAREFGGGGH
                     AVAAAATVKGQTLAEVKERVIRLLTTHHRPTLLAQDVMTRPVKAIEVDTSVTEAGQRM
                     TAYGLNVFPILDEKDHYTGLVSRESIQKALFHRLGKIPVRDIMQTDAYIAQPDTPFHE
                     IETAMIERNQRFVPIIQEKKIIGVITRTDLLRTLHDDVLKVARMRTMRPSEAKAEIGG
                     PRRNVKGLLKNRLPQRVATVLEDAGQLADRCQVPLFVVGGCVRDLLLGIENLDLDLVV
                     EGDGIAFARKLGEVLHARVKVHERFGTAILLAEDGFRLDVATARTEYYEYPTALPTVE
                     QSSIKKDLYRRDFTMNALAVRLNGKGFGDVLDFYGGQRDLNDKMIRVLHGLSFVEDPT
                     "
     gene            2104..>2402
                     /locus_tag="VN039_03210"
     CDS             2104..>2402
                     /locus_tag="VN039_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45030.1"
                     /translation="MKIHRVKILPGVLGGLAVLSLVIPSITAANSKDGKRQNARSSSA
                     AAAAEVMVSTRETASVSTADPSDRKPGPAWKTIGGTIKHIKGDAYTVEDYEGNQV"
BASE COUNT          501 a          730 c          627 g          544 t
ORIGIN      
        1 gtcggatctt caacgaaact cagcccgtgc aaaacgcgga tcatcttgtc attcagatcc
       61 cgttgcccac cgtagaaatc gagcacatct ccgaaaccct ttccgttcaa ccggacggcg
      121 agcgcgttca tggtgaaatc ccggcgatag agatctttct tgatggaact ttgctccact
      181 gtcgggaggg cggtcggata ttcgtaatat tccgtccttg cggtggccac atcgagcctg
      241 aagccgtcct ccgccagcag gattgccgtg ccgaatcgtt catgtacctt gacccgtgcg
      301 tgcaacactt caccgagctt tcgcgcaaac gcgattccat ccccttcgac cacgagatcc
      361 aaatccagat tctcgatgcc gagcaacagg tctcgcacgc agccgccgac gacaaagagc
      421 ggaacctggc aacgatcagc caattgtccc gcatcttcca gcaccgtcgc cacgcgctgc
      481 ggcaagcggt tcttcaacag ccccttcaca ttgcggcgcg gtcctccaat ctcagctttg
      541 gcttcgcttg gtcgcatcgt ccgcattcga gcgaccttca gtacatcgtc gtgcaaggtt
      601 cgcaacagat ccgtcctcgt aatgacgccg atgatcttct tctcttggat gatcggcaca
      661 aaccgctggt ttcgctcgat catggccgtt tcgatctcat gaaacggagt gtccggctgc
      721 gcgatatagg cgtcggtctg catgatgtcg cgcacgggaa tcttgcccag ccgatgaaac
      781 aaggcttttt gaatcgactc ccgactgaca agcccggtgt aatggtcttt ctcatccaag
      841 atcggaaaga cattcagccc ataggccgtc atccgctgtc ctgcttcggt cacgctggta
      901 tcgacctcaa tcgctttgac cggtcgggtc atgacatcct gtgccagcag agtcgggcga
      961 tggtgggtgg tcagcagccg aatgaccctc tctttgactt ccgcaagcgt ctgccctttg
     1021 accgtggcgg ctgccgccac ggcgtggcct ccccctccga attcccgtgc gatccagccg
     1081 acgtcgatct caggccttcg gcttctcccg atcacttcca ctcggtctgc catcatcacc
     1141 gccacaagga cggcatcgac cccttgcaac tcggcgagcc tatgtaccac gccggctgct
     1201 tctccgcggc agcgatcaac cgtgctcgtg gccaccaaga tctttcggcc ttccagataa
     1261 tgcacttcgc tgtgttccaa aaaatcgttc agcaatgcga cggcatcagg atccagagga
     1321 cggtgcagga tctccgtcac catattcaga tccgcaccgg ctgcggcgag aaacgcccct
     1381 gcctcaaaat cccgactggt cgtggaagta aaggtaaacg acccggtctc ttcatagagg
     1441 cccagggcca gcacagtcgc ctcgaacggc gtcacgggaa tatgccgccg ccggagttgc
     1501 tctatcagaa gtgtcgtcgt tgccccgacc gattcgatca cagactgttt cgaacggtcg
     1561 gtgcaggatg cgtgtggttc aaggtggtga tcgaacacga cgacttcgac cgccggattc
     1621 tcgatgcagg atttgagcgc tccgattcgg tcaggctctt gggtatcgac cagcaccagc
     1681 ctggtgacct tcgagagatc gatatctttg agtttggtta tgtcgagatc gtgcacggca
     1741 agaaaattac gaactgcttc ctggccccca cccggcaaga ccagcttggc ctctggaaag
     1801 agtttgcgcg cggcgaccac agaggccagg ccgtcgaaat ccgcgttgct atgcgtggct
     1861 atgacatcca cgagcgcatt ttagcaggat gataccctcg tcggcgttcg cctgatccac
     1921 aggtgacggt cgccgtgcag ttttgctcaa tgagatgacg ttttttctcc ggcctcatgg
     1981 gaaacgcagt gttttttcag cagcaaattc ggactggtaa tcatcttttc catctttctt
     2041 cgatcggcac gtcctttgct gcccactcgt acaattcaca tcctttgtac caggaggtgc
     2101 tcaatgaaga ttcatcgcgt gaaaatcctt ccgggtgtcc tgggaggtct agccgttctt
     2161 tctttggtta taccgtctat aacagccgca aattcaaaag acgggaaaag gcagaatgcc
     2221 cgttcgtcgt cagccgcagc tgcagcagag gtgatggttt caactcggga aactgcctca
     2281 gtctctactg ctgacccgtc ggaccgtaag ccgggtccgg cgtggaagac aatcggcgga
     2341 accatcaaac acatcaaggg cgatgcctat acggtcgaag actacgaagg aaatcaggtc
     2401 ca
//
LOCUS       DATMTI010000193         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_220834, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000193 DATMTI010000000
VERSION     DATMTI010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220834"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="VN039_03215"
     CDS             complement(<1..1943)
                     /locus_tag="VN039_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV45031.1"
                     /translation="MIDRIVEISLIQRFLVCTLGFLLLFGGLYAFHLLDIVAYPDPSA
                     PMVELITQLPGYSAEEIERQITIPIEVALSGIPGLTDIRSLSIFGLSDVKVYFDFDTG
                     IFQDRQEVLNRLDSVQLPQGVRPVLSPWWTIAEIYRYELTGQETSLTDLKTIQEWQVR
                     RAFKRVSGVIDVTTFGGTTKEYHVDIDPGKLISYGVNLSQVMAALANSNANVGGNYLT
                     IGAQSYNIRGLGLINDLDDIENVMVAEKDGTPIFVKTLGKVTVGHRVRLGKVGIDDRD
                     DVVEGVVLQQRGYKALPVLDKVRAKVEELNGLKLPSGVKIKAFYDRTALINTTVKTVT
                     DILISGMVLVFIILVVFLGDLRAAFIVALTIPLSLLFTFTMMVFVGESANLISLGAID
                     FGIIVDATLVMVECIFLQLAHRKPHGLTIHQYIIRAAGQVGRPIVFSTAIIVVAFVPL
                     FTMTGVPGKIFAPMSITYGFALLGALLMACTLAPVLCSFLLRSSIRETDTAVVAVVRK
                     ANARVVVWALEHRIMVVGVATGLVILALLAFQSLGGEFMPPLEEGNLWVRATMPVDIS
                     FDQADRLTNEIRRAFRESPEVETVVSQLGRPDDGTDPTSFFNGEFLANLKPASEWRRG
                     LSKQDLIEEIEHRLKTIPGVIFN"
     gene            complement(1992..>2609)
                     /locus_tag="VN039_03220"
     CDS             complement(1992..>2609)
                     /locus_tag="VN039_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV45032.1"
                     /translation="PEELSKPLDKQRITSRFEMKSPLTGVVVERTVTPGQSVTGDADH
                     VLFTIADLDVLQVVADVYERDLALVREGQSAVVKVEAYPDVDFPAKVIAVGDVVDPST
                     RTIKVRALVTNEAHELKPEMFARLQLDVSGIGPFLTVPREAVLEADGKQFVYVVEEGN
                     RYVKRDVKTVNISPDQTRVLAGLTQGERIVTKGAVLIKGQQVEGT"
BASE COUNT          619 a          755 c          662 g          573 t
ORIGIN      
        1 aaattgaaga taacgccggg gatggttttc agccgatgct cgatttcttc aataagatct
       61 tgcttgctca atcctcgacg ccattccgat gcgggcttaa gattcgccaa aaattctccg
      121 ttaaaaaagc tggttgggtc cgtcccatca tccggccgtc ccagctgtga aacgaccgtt
      181 tccacttcgg gcgattctcg gaacgcgcga cggatttcat tggtcagtcg atcggcctga
      241 tcaaaagaaa tatcgacggg catcgtggct cgaacccaga ggtttccttc ctccagaggc
      301 ggcatgaatt caccacccaa cgattggaat gccagaagcg cgaggatcac cagacctgtc
      361 gcaacaccaa ccaccatgat ccgatgctcc aacgcccata cgacaaccct tgcattggct
      421 ttgcgtacga cagcgaccac ggccgtatcg gtttctctga tcgacgaccg caagaggaat
      481 gaacacagca cgggcgcgag cgtacatgcc atcaagagtg cgccgagcag agcaaaccca
      541 tatgtaatgg acatgggcgc gaagatcttc ccagggactc cggtcatcgt aaaaagcggc
      601 acaaaagcca ccacgatgat cgccgtggaa aagacaatag gccttccgac ctgaccggcg
      661 gctctgatga tgtattggtg aatcgtgagc ccatgtggct tccggtgcgc taattggaga
      721 aagatgcatt cgaccatcac caacgtcgcg tccacgatga tcccgaagtc gatcgcgccc
      781 aaggagatca agttcgcaga ctccccgacg aacaccatca tcgtgaaggt aaataacagc
      841 gagagcggga tggtcagtgc gacaataaag gccgctcgga gatcgccgag aaatacgacc
      901 aagatgataa acacgagcac catgccgctg ataagaatgt ccgtgaccgt cttcacggtc
      961 gtattgatca acgctgttcg atcgtagaac gccttgatct tgaccccgga cggcaatttc
     1021 aacccattca actcctcgac cttggctcgt actttatcga ggaccggtaa ggccttatag
     1081 ccgcgttgct gcagcacgac gccttccacc acgtcatctc gatcatcgat ccctactttt
     1141 ccgagacgta cgcggtgccc tacggtcacc tttcccaagg tcttgacgaa gatcggcgtt
     1201 ccatcttttt cagccaccat cacgttttca atgtcatcga gatcattgat gaggcccagc
     1261 cctctaatgt tgtagctctg cgcgccgatc gttaaatagt tgccgccgac attggcgttg
     1321 ctgttcgcca aggcggccat gacttgagaa agattgacgc cgtagctgat cagtttcccc
     1381 gggtcgatat cgacatgata ttctttcgtg gtgccgccga acgtcgtcac gtcgatgaca
     1441 ccagacacac gtttgaacgc ccgccggacc tgccactctt gaatggtttt tagatcggtt
     1501 aaactcgtct cctgaccggt caactcatac cgatagatct cggcaatggt ccaccaggga
     1561 gaaagcacgg gccgcacgcc ttgaggcagt tgaacggaat cgagccgatt caacacttcc
     1621 tggcggtctt gaaagatgcc agtatcgaaa tcgaagtaca ctttgacgtc actgagaccg
     1681 aaaatcgaaa gcgaccggat atcggttaat cctgggatcc cgctcaaggc gacttcaatg
     1741 gggatggtaa tttgcctctc aatttcctcg gcggaatatc ccggaagttg ggtgatcaac
     1801 tccaccatcg gagcggaagg gtccggatag gccacaatgt cgaggagatg aaatgcgtag
     1861 agccccccga acaagagcag aaacccaagg gtgcagacca agaaccgctg tattagtgag
     1921 atctcgacga ttctgtcgat catggattca ttgcactcgg cggtgttgct gaggcaggat
     1981 ggactcgtcg attaagtgcc ctcgacctgt tgccctttta tcaaaactgc gcctttggtc
     2041 acgattcgct ctccttgcgt cagtcctgcg agaacgcgag tctgatccgg cgagatattc
     2101 acggtcttca cgtcccgttt cacataccga ttcccttctt caaccacata gacaaactgc
     2161 ttgccgtccg cttccaggac cgcttcgcgg ggcacggtga ggaatggtcc gatgccgctg
     2221 acatcgagtt gcagccgagc gaacatttcc ggtttcagtt cgtgcgcctc atttgtgacg
     2281 agagcccgca ccttgatcgt tcttgtcgaa ggatcgacca cgtctcccac cgctatgact
     2341 ttggccggaa aatcaacatc aggatacgct tccactttca cgacagccga ttggccctcc
     2401 cgtacaagcg cgaggtcacg ctcgtagaca tcggccacaa cctgtagcac atcgagatcg
     2461 gcgatggtga acagcacatg gtcggcatct ccggtcaccg actgaccagg cgtgacggta
     2521 cgttcgacga cgaccccggt caacggactc ttcatctcga atcgcgacgt gatccgttgc
     2581 ttatccagcg gcttgctcaa ctcttccgg
//
LOCUS       DATMTI010000194         5202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_305474, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000194 DATMTI010000000
VERSION     DATMTI010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305474"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VN039_03225"
     CDS             <1..495
                     /locus_tag="VN039_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45033.1"
                     /translation="VRRGFRAQLRTGNFLAGQLYVALDFFPHALPVQLDPNRTPREIP
                     TVVSGIEDLQSQLAEIAQKLNKVPFDSIAANLDKSLTGLKSMLGNAEKLFKQVNEVTP
                     EAKAALAEARKSFTAAQHTLSQDAPLVHDARQAVQELTRSAQSLRVLADYLERHPESL
                     LRGK"
     gene            510..1163
                     /locus_tag="VN039_03230"
     CDS             510..1163
                     /locus_tag="VN039_03230"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HWV45034.1"
                     /translation="MSTPTQRAVVLFILGMVMSAAGCAHATQERFYTLSDSMPDGTAD
                     APIGNNFVEILPVSLPELLDRPQVVLYDEAGRVHILEEDRWAATLKSEIRRSIGEKVR
                     HRLGMIDVYHTPAPHGESVKGKTYRITVSVESFKVAPGHGTMMQAVWAVRSIDGNADF
                     VCRSAYQMELGERSLSHAILAYRKAIDALGTRIAQSIRNAAQGPRDGEAEQGECVPR"
     gene            1546..2253
                     /locus_tag="VN039_03235"
     CDS             1546..2253
                     /locus_tag="VN039_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV45035.1"
                     /translation="MALRGTVVLIAGGAGALGGTVTPAFAQSGARVITVERNPPPDQV
                     AAGNAMQADVTNETALRRLVHDVIQTAGRLDVLVNLVGGFAMGRVVDTDVSLWQRMLT
                     MNVTSAFLLSKAVLPHMLERGTGRILHVAAWAAVEPFPGAAAYLVAKSSLLALIRVSA
                     LELKGSGVTVNGVLPTTIDTPANRASMPHTDPSMWTKPESIADTLLFLASEEAGQING
                     AMLPIGTGGGATSAGKP"
     gene            2274..2702
                     /locus_tag="VN039_03240"
     CDS             2274..2702
                     /locus_tag="VN039_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45036.1"
                     /translation="MSGWDRERAHLNNLSQALLRLHKALLDAERLSYERVHGRIASNG
                     EFLQLVLEDSWFAWLRPLSHYAAGLDELTEGRRTASGTEITALLDAVRSLLRPSEGSE
                     GFGGQYYDALQRVPEVGLAHAQVKALLGYEIHTGRLHDED"
     gene            2689..3153
                     /locus_tag="VN039_03245"
     CDS             2689..3153
                     /locus_tag="VN039_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HWV45037.1"
                     /translation="MMKTDLEKVKTLAKDLRKSYPRSPREKLGGYVIAARCADKCRAF
                     LLDMNGEYNYWPCSLASQWFSFTGIRPEQFKEVVETGATDEELASWIAGHSKVKDPDE
                     VLKWNNAMRDTRLSDMSLQAQQYLEEYIPKFVPNHRPVYVWFDVYDLEEKRF"
     gene            3507..>5202
                     /locus_tag="VN039_03250"
     CDS             3507..>5202
                     /locus_tag="VN039_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWV45038.1"
                     /translation="MAYLPIKDYGLIGNMRTAALVGLNGSIDWLCIPHFDSPSVFAAI
                     LDDEKGGRFEITATDTDISPKQLYWPDTNVLVTRFHAPTGIGEILDFMPVGLPPDSPW
                     HHQLIRRVRVTQGSMEFRVRCHPAFNYARTPHHTQVTKQGARFHSPELSLGLATDIPL
                     AQDDRGAQAVFFLQEEESAVFVLRLIEADESSGSWPPLGEVDDMFEHTVRYWRRWLSR
                     CTYTGRWREMVYRSALCLKLLTFEPTGAIVAAPTCSLPETPGGVRNWDYRYTWIRDAA
                     FTVYGFLKIGFTEEADRFLKWLAARAKEVEADGSLQIVYGIDGRHDLTEQTLDHLDGY
                     RGAKPVRIGNGAYNQLQLDIYGELLDAVYLYNKYVTPIGHDSWRRIRHRLDWLCDHWQ
                     QPDEGIWETRGGRRQFVYSKLMCWVAMDRGLRLADKRSFPADRARWLGVRDQIYEEIM
                     ERGWSAKREAFVQHYDSDSLDASNLIMPLVFFLSPNDPRMLDTLGAIMRSPKSGGLVS
                     DGLVYRYDSETGEDGLHGKEGTFNMCTFWLVEALSRAGRVDRTKLEEARFLFERMLG"
BASE COUNT         1094 a         1420 c         1598 g         1090 t
ORIGIN      
        1 gtccgccgcg gctttcgcgc ccaactgcga acgggaaatt ttttggccgg tcaactctat
       61 gttgctctcg actttttccc gcacgcgttg ccggttcagc ttgatccaaa cagaacgcct
      121 cgcgagatac ccacggtcgt cagcggaatt gaagatctgc agtctcagtt ggccgagatt
      181 gcccaaaagc tgaacaaggt cccgttcgac agcatcgctg cgaatctcga caaaagcctg
      241 acaggattga agagcatgtt gggaaatgcg gagaaactgt tcaagcaagt caatgaagtg
      301 acgccggagg ccaaagccgc actcgccgaa gcgcgcaaat cattcactgc ggctcagcac
      361 accttgtcgc aagatgcacc acttgtgcac gatgcgcggc aagccgtgca agaactgact
      421 cgctccgccc aatcgttgcg ggtcttggcc gattatctcg agcgtcatcc tgaatcctta
      481 cttcgaggaa aatagagagc ggggttgaag tgagtacacc gactcaacgg gctgtcgtcc
      541 tgttcattct tggaatggtc atgagcgccg cgggatgtgc ccatgcaacc caggagcggt
      601 tctataccct ctctgattcg atgccggatg ggacagcgga cgcaccgatc gggaacaact
      661 tcgtagaaat cttgcctgta tccttgccgg aattactcga ccgccctcag gtcgtgctgt
      721 acgacgaggc cggccgagta catattctgg aggaagatcg atgggcggcg acgctcaaaa
      781 gcgagattcg ccgatccatc ggcgagaagg tgaggcaccg gcttgggatg atcgatgtgt
      841 atcacacgcc tgcccctcat ggggaatcgg tcaaaggcaa aacctatcgc atcaccgtca
      901 gtgtcgagtc gttcaaggtc gctccgggcc atgggacgat gatgcaagca gtctgggcgg
      961 tccgctcgat cgatggcaac gcagacttcg tctgccgctc ggcgtatcaa atggaattag
     1021 gcgagcggag tctcagccac gctatccttg cctatcgcaa agctattgat gcactcggca
     1081 cccgtatcgc gcagagcatt cggaatgcgg cgcaggggcc tcgggatgga gaagccgaac
     1141 aaggcgagtg tgtcccacga tgaggccgac gtaagatgta ggtctcctcc ttcctcctcg
     1201 tcatgttgac cctggccgaa tccggccgca aagcccagtt gccgttagct caccgtcctt
     1261 cttgcgacac gaaggcatga aatcgtccgc gcgagcgagt ttgctgcgca ggcctcggcc
     1321 acttctttgg cggaacttaa cgacccgact cgattcgcaa tggagggagt tgcacagatc
     1381 atccgacctc atgtattgga tagaacagaa aggcggtgga cgaactttca tcctggacat
     1441 tgcagcgcgt aggggagagt caatcctgcc gcgatgtcaa ggggaggctc atcagctcgg
     1501 atagcgagcc gatggatgag acggcaaccc aaagggaaca gacctatggc gttaagggga
     1561 acagtcgtgc tgatcgccgg aggagccggt gcgttgggcg ggacggtcac accggccttt
     1621 gcccaatccg gcgcacgcgt catcactgta gagcgcaatc cgccgcctga tcaggtggca
     1681 gcggggaatg cgatgcaagc cgatgtcacc aatgagacgg ccctccgtcg cctcgtccat
     1741 gacgtgatcc aaacggcggg ccgtctcgat gtgttggtga acttggtcgg cggctttgcg
     1801 atggggcgtg tcgtggacac cgatgtgtcg ctgtggcagc ggatgttgac gatgaacgtg
     1861 acgtcggcgt tcctcctgtc gaaagccgtc ctgccccata tgctggaacg cggaactggt
     1921 cgtattctgc acgtggcggc ttgggcagcg gtcgagccgt ttcccggcgc cgccgcctat
     1981 ctcgtcgcca agtcgagcct gctcgcgctg atccgcgtgt cggcgctcga actgaaaggc
     2041 tcgggcgtga cggtcaacgg ggtgctgccc accaccatcg atacgccggc caaccgcgcg
     2101 agtatgcctc acactgatcc gtcgatgtgg acgaagccgg agtccatcgc cgacacgctg
     2161 ctctttctcg cctcagaaga agccggccag atcaatgggg caatgcttcc gatcggcacc
     2221 ggaggcggcg caacatcggc gggcaagccg tagcaacggc aaggaattgg attatgtcgg
     2281 gttgggatcg cgaacgggcg cacttgaaca atctttctca ggcgctgctc cggttgcata
     2341 aagcattgtt ggacgcggag cggctgtctt atgagcgggt acatggccgc attgcgtcca
     2401 acggagaatt tcttcaattg gtgctggagg attcctggtt tgcatggttg cggccattgt
     2461 cccactatgc agccggcctt gatgaactca ccgaagggcg ccggactgcg tccggcaccg
     2521 agatcaccgc tctgcttgat gcggtacgga gcctgctgag gccgtctgaa gggagtgaag
     2581 gattcggcgg gcagtattac gatgcattgc aacgggtgcc tgaagtcggg ttggctcatg
     2641 ctcaagtcaa ggcgctgctt ggttacgaga tacacacagg gaggctgcat gatgaagact
     2701 gatctcgaaa aggtgaagac attggccaag gatctccgaa agagttatcc ccgcagcccg
     2761 cgagagaagc tgggtgggta cgtgattgcg gctcgatgtg cggataagtg tcgggcattt
     2821 ttgctggaca tgaacggcga gtacaattac tggccctgct cgctggccag ccaatggttc
     2881 tcgttcaccg gcatcaggcc ggaacagttc aaggaagtcg tcgagaccgg agcaaccgat
     2941 gaagagctcg cgtcttggat cgcgggtcat tccaaggtca aggatccgga cgaagtgttg
     3001 aagtggaaca atgcgatgcg cgatacaaga ttgagcgaca tgagtctcca ggctcagcaa
     3061 tatctcgaag aatacattcc caagttcgta ccgaatcatc ggccggtcta tgtgtggttt
     3121 gacgtgtatg acttggagga aaaacggttc tagcaggctg ctgaaaaagt ccgccagcgg
     3181 cgttctcgca tcgctcagag actcaacgta ccgaagcgta cgcctcgtct cttcgcttgc
     3241 tgcggccttg ctggacagcc tttttgagca tcctgaaggc atccttgcgt tggcactata
     3301 cggacgatta gaaacacgtt catcgatggt ctccattttc ggcgatccga tgtccgatca
     3361 gcatacgatc gagttcttgt gtgtcgcggg ttagagatgg accgccggca agtgttataa
     3421 ccagaaatga gagaagcgag gttgaacggc cggcggacgc aatgggtcgt ccgggtggat
     3481 cggatcaggc tggttcggta taagacatgg catatctccc gatcaaagac tacggcttga
     3541 ttggcaacat gcgcacggcg gcgctcgtcg gtctgaacgg atcgatcgat tggctgtgca
     3601 ttccgcattt cgactctccc agcgtgtttg cggccattct cgacgacgag aaaggcggcc
     3661 ggttcgagat cactgcgacc gacacggaca tctctccaaa gcagctctac tggccggata
     3721 ccaacgtgct tgtgacgcgc ttccatgcgc cgaccggcat cggcgagatc ctggatttta
     3781 tgcccgtcgg tttgcctccg gactcgccat ggcaccatca actgattcgc cgggtccgcg
     3841 tgacgcaggg aagtatggaa tttcgagttc gttgccatcc cgccttcaac tacgctcgaa
     3901 ctcctcatca cacccaggtg acaaagcaag gagcgaggtt tcattcgccg gagctcagtc
     3961 tggggttggc gacggatatc ccgctcgcac aagacgatcg aggagctcag gcagtatttt
     4021 ttttgcaaga agaggaatcg gcggtgttcg tcttgcggct cattgaagcc gacgaaagca
     4081 gcggctcctg gccgccgttg ggcgaagtcg atgacatgtt tgagcacacc gtgagatatt
     4141 ggcgccgttg gctctcgcga tgcacctaca ccggccggtg gcgggaaatg gtctaccgat
     4201 cggctctgtg tctcaagctc ctcacgttcg agccgaccgg cgccatcgtg gccgcgccga
     4261 cctgtagttt gcccgaaact cccggcggcg tgcgcaattg ggactatcgg tacacctgga
     4321 tacgcgatgc ggccttcacc gtctacggct tcttgaaaat cgggttcacc gaggaagcgg
     4381 atcgcttcct gaaatggttg gctgcgcgcg cgaaggaggt cgaggcggac ggctcattgc
     4441 agatcgtgta tggaatcgac ggccgtcacg atttgacgga gcaaacgctc gaccatttgg
     4501 acggctaccg cggcgcgaag cctgtgcgga tcggcaacgg cgcgtacaac cagttgcagt
     4561 tggatatcta cggcgaacta ctcgacgccg tctatctgta caacaagtat gtgacaccga
     4621 tcggtcacga ctcctggagg agaatcaggc atcggctcga ctggttgtgc gaccactggc
     4681 aacagccgga tgaaggaatc tgggagacca ggggcgggcg tcgacagttc gtctattcca
     4741 agttgatgtg ttgggttgcg atggaccgag gccttcgctt ggccgacaaa cgctccttcc
     4801 ctgccgatcg cgctcgttgg ctgggcgttc gggatcagat ttacgaggag atcatggagc
     4861 ggggatggag cgccaagcgg gaggcgttcg ttcagcatta cgacagcgac tcgttggacg
     4921 cctcgaatct catcatgccg ctcgtctttt tcctttcacc gaacgatccg aggatgctcg
     4981 ataccttggg ggcgattatg cgctcaccga aatccggcgg actggtatcc gatgggttgg
     5041 tgtatcggta cgacagcgaa acgggagaag acggccttca tggaaaagag ggaaccttca
     5101 acatgtgtac cttttggttg gtggaagcct tgtcccgcgc aggacgagtc gatcgcacca
     5161 aacttgaaga agcgcgcttc ctgttcgaac ggatgttggg tt
//
LOCUS       DATMTI010000195         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_814647, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000195 DATMTI010000000
VERSION     DATMTI010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814647"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VN039_03255"
     CDS             complement(<1..912)
                     /locus_tag="VN039_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45039.1"
                     /translation="MRQRAMKVQRICTIASVILLMWDVFLCSSGGGSLAASDPLVLPL
                     RLEVSESSTASGPSEQSAASVQALAIGGNGVIYAGSFGHGIFRTVDRGATWARVGDGI
                     TDPFILSLASMKDGAVYAGTFRGGVFRSRDEGKSWQPVNAGLNRLEVKALLAVDHELF
                     AGTGDGVYRLHGSEDHWTPVTSGLDDILVHALARSADGTLFAGTSGKGVFRFSPRSTG
                     WVRMRHGLKDHEGLIENFIRVLVIDQDQSIFAGTFDGGVFRSADGGLTWRSISRALPN
                     DSIRGIVFSDQGVVVATGNGIFKTVDKG"
     gene            complement(909..1685)
                     /gene="tatC"
                     /locus_tag="VN039_03260"
     CDS             complement(909..1685)
                     /gene="tatC"
                     /locus_tag="VN039_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWV45040.1"
                     /translation="MEQTTHPLASHIQAVKRRLIIIGATVLGSLVLTFSFSTEMVAWL
                     NRPFSNQLAFYGPTEALFASIKVSLLAALILSLPVIFYQCWKFIEPALLPKEQRWAIP
                     LFMVAGGLFALGLIFCNVVILPLVIDLFVSFGLDRDITPQLSVGTYIDFNVKFLLIFG
                     CAFELPLAMSLMAMTGVVSADMFARYRKHAVLSCLIISAIVTPDATLFTMLLMAVPLM
                     ALYEIGILGARLFGRNQDPEGIDLPLDPDLPINTAGTRLR"
     gene            complement(1731..1970)
                     /locus_tag="VN039_03265"
     CDS             complement(1731..1970)
                     /locus_tag="VN039_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HWV45041.1"
                     /translation="MLTEDAIVEQLRHSNTQFRELEASHHRLDHELNELQKRHVLTPS
                     EEIEKKRMQKEKLAKKDKLAELIRLYREQRLEPAR"
     gene            complement(1986..>2558)
                     /gene="rimI"
                     /locus_tag="VN039_03270"
     CDS             complement(1986..>2558)
                     /gene="rimI"
                     /locus_tag="VN039_03270"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWV45042.1"
                     /translation="AGNVSSYNVLSGCEIVPATPDMLPDILALEEACFSSPWTRKMLE
                     GELTSNHFARFLVARQQAESTTKAEPIIIGYHCFWIVFEELRLMNLAVRESMRKRGVG
                     GALVTEALCLGLEQAATRAVLEVRASNDPARSFYSRLGFVQIGSRPRYYTNPIEDAVL
                     MEMNPLVMSNGRQRDAGFVSGGESASTDSL"
BASE COUNT          635 a          756 c          648 g          519 t
ORIGIN      
        1 gcccttatcc acggtcttaa aaatcccgtt cccagtcgcc acaaccactc cttgatcgct
       61 gaatacaatg cctcggatag agtcattggg aagcgcccgg ctgattgatc gccacgtcag
      121 gccgccgtcg gcgctgcgaa acactccgcc gtcgaacgtg ccggcaaaaa tactctggtc
      181 ctgatcgatc accaaaacac gaatgaaatt ttcaatcagt ccctcatggt ccttcaaccc
      241 atgccgcata cgaacccaac cggtagagcg ggggctgaag cgaaagacgc ctttaccgga
      301 agtccccgca aataaggttc catcggcaga gcgagccagt gcgtggacca gaatatcgtc
      361 caaccccgac gtcacaggcg tccaatgatc ctcggagcca tgcaggcgat acacgccgtc
      421 tccggtgccg gcaaaaagtt cgtggtccac cgccagtaat gccttgactt caagccgatt
      481 gagtcccgca ttgaccggct gccagctctt cccttcatcg cgcgaacgaa acactccacc
      541 acgaaacgta ccggcgtaga ctgctccgtc tttcatggag gccaaactca aaataaaagg
      601 atcagtgata ccatctccta ctcgagccca cgtcgcaccg cggtccacgg tgcggaagat
      661 cccatgcccg aaggatccgg catagatgac cccattgcca cctatcgcaa gggcctgcac
      721 gcttgctgcg gattgttcgg atggtcccga tgccgtcgaa gattctgaaa cctccaaccg
      781 tagaggcaac accagcggat cggaggctgc aaggctccct ccaccggacg agcagagaaa
      841 aacatcccac atcaataata tcacagaagc gatcgtgcat atgcgctgaa ccttcatagc
      901 cctctgcctc atcgtaatct cgtcccggcg gtgttgatcg gcagatccgg atcgagcggc
      961 aaatcgatcc cctctggatc ttgattccgt ccgaaaagtc gggcgccgag aatcccgatc
     1021 tcatacagcg ccatcaaggg aaccgccatc aaaagcatgg taaagagcgt cgcgtcagga
     1081 gtgacgatgg cggagataat aagacacgac aaaacggcat gtttacggta acgagcgaac
     1141 atgtctgcgg ataccactcc agtcatagcc atgagactca tggccaaggg caattcgaag
     1201 gcacagccga agatgagcag gaatttcacg ttaaaatcga tgtaggtgcc gacgctcagc
     1261 tgcggggtga tatcacgatc gagtccgaaa ctcacgaaca ggtcgatcac cagcggtaaa
     1321 atcaccacat tgcagaagat cagccctaac gcgaacagcc cgcccgccac catgaatagc
     1381 gggatcgccc agcgctgttc tttcggtaac agggcgggct cgatgaactt ccaacactga
     1441 taaaaaatga ccggcaagct cagaataagg gctgccaaca acgacacctt gatcgaagcg
     1501 aacagcgcct ccgtggggcc ataaaacgcc agttggttcg aaaatggtcg gttgagccac
     1561 gcgaccattt cggtggaaaa cgagaaggtc agcaccaacg aacccaacac cgtcgcacca
     1621 atgatgatca gtcgtcgctt gacggcttgg atgtgggaag caagcggatg ggtggtctgt
     1681 tccatatcga cagattcagc cgcctgcaga atcggtggcc gatcagcttc ctaccgcgcc
     1741 ggctcgagcc tttgctcgcg gtagaggcga atcagttctg caagcttgtc tttcttcgcc
     1801 aatttttctt tttgcatccg tttcttctcg atttcttcgg agggggtcag aacatggcgc
     1861 ttctgcagct cgttgagttc atggtccagg cgatggtggg acgcttctag ctcccggaat
     1921 tgggtgttgg aatggcgaag ctgttcgaca atcgcatctt ccgtcaacat cacgacacct
     1981 cctggttaaa gtgagtccgt tgaagctgat tcacctcctg aaacaaagcc cgcatcccgc
     2041 tgccggccat tcgacatcac aagtggattc atttccatca gcacggcgtc ttcgatggga
     2101 ttcgtgtaat aacgagggcg actgccgatc tgcacaaaac ctagacgact atagaaggaa
     2161 cgcgctggat cgttcgaggc tctgacttcg agcaccgccc gagtggccgc ttgctctaat
     2221 ccaagacaga gggcttcggt gacaagcgcc cctccaaccc ccctctttcg catcgactct
     2281 cgcaccgcca aattcatcaa tcgcagctct tcaaagacga tccagaaaca atggtatccg
     2341 ataatgatgg gttccgcttt tgtcgtcgac tccgcttgtt gcctcgcgac aagaaagcgc
     2401 gcgaaatggt tgcttgttaa ctcaccttcc agcattttgc gcgtccaagg ggacgagaag
     2461 caagcttctt ccaaagcaag gatgtccggt aacatatccg gtgtggccgg gacgatttca
     2521 catccagaaa gcacgttgta gctgctcaca ttccctgc
//
LOCUS       DATMTI010000196         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_771889, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000196 DATMTI010000000
VERSION     DATMTI010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771889"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VN039_03275"
     CDS             complement(<1..665)
                     /locus_tag="VN039_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWV45043.1"
                     /translation="MSDPREPIIRFDHASFGFPGLIALQDISLTIYEGEFVGVIGPNG
                     SGKTTLCRAVLGLLAPVEGHLHIFDCACDELRCHHRAKIGYLPQKGVVDRNFPVTVLE
                     TVMMGRYGALGLFKRPGRKDHDIALEALAQVGMESHKDHALGHLSGGQQQRVFIARAL
                     AQQPKVLILDEPTTGLDITTQHNVIELVQHLHGELKLTVLLITHDINMFRSRVDRLVL
                     LKT"
     gene            complement(704..1654)
                     /locus_tag="VN039_03280"
     CDS             complement(704..1654)
                     /locus_tag="VN039_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWV45044.1"
                     /translation="MIPSPPMFPANHVRKSPVFSLLAVLAGFLLSFGTITEARDPIPV
                     VVTIPVLKDLAEQVGGPHVRVTSLLSGYENEHTYSPKPTDLVAVRKARLLLEIGMGLE
                     VWVSSLVKNAGDPSLRVITTSQGVALIRDDADSHEGTHHEGEAGNPHIWLDPENVPIM
                     LRHITQAFIEVDPRHAADYQANQTAYLRRLGQVQQELLERTQRLSDRRFIAHHPAWPY
                     LAKRFSFNIVGTIHRQSGTEPSAYHLQFLIEKIRKENIKVVVSEVQLSRRLPELLARE
                     TKARVVVLTAMPGGLAGTETYLDMLRYDVLQLAGALETTS"
     gene            1959..3158
                     /locus_tag="VN039_03285"
     CDS             1959..3158
                     /locus_tag="VN039_03285"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWV45045.1"
                     /translation="MKVVGLMSGTSADGVDAALVTIARQRAGIRVGMLAFHSLPYPRS
                     LQQRILSASVSGTVTDICHLNALLGEWFADAALGVIRSAQLSPEDIDLIGSHGQTVHH
                     LPHGIKDTRVGAIRSTLQIAEPAVIAERTGITTVADFRPRDIAAGGQGAPLTPGSHAL
                     LFRHPRRARLIVNLGGISNVTYLPRGSGSENLVAFDTGPANMVLDGLMSRTTNGRASM
                     DRDGRLAAKGLVDSRLLAKLLAHPYLSQAPPKSTGREAFGAKMLDELLNWQQTRRVSV
                     EDLLATCSRWTAEAVGTARRWIRGGIDDVIVGGGGVRNRAIMGHLTDIFAPVSVAPFE
                     SHGWDSKALESVAFAVLAYQTVMEQCGNVPSVTGAASPRLLGCIVPSGPRWYERLRSR
                     KGGSKTR"
     gene            3155..3661
                     /locus_tag="VN039_03290"
     CDS             3155..3661
                     /locus_tag="VN039_03290"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HWV45046.1"
                     /translation="MKILLAGAVIAVSIGLWGYLRRVRRRKKQASPFAVPHDATISSV
                     RLLAQEEVALYSLLQMAVQERYLVFSQVPLWSFVEVEAAAQVRSEVLRQIALKRVDFV
                     LVHPGSCLVEQVVQIERESSQPHEAERQHVIESVLDAAGIKLTKLQPKQTHSLPDLIA
                     RLGLVEEE"
BASE COUNT          805 a         1041 c         1130 g          785 t
ORIGIN      
        1 ctggttttga gaaggaccaa tcgatccacc cgcgagcgga acatattaat gtcgtgcgtg
       61 ataaggagga ccgtgagttt gagctcgcca tgaaggtgct gtaccaactc gatgacattg
      121 tgttgggtgg tgatatccag accggtcgtc ggttcatcca atatcaggac cttgggttgc
      181 tgggccagtg ccctggcgat gaagacgcgc tgttgctggc cgccggacag atgcccgaga
      241 gcatgatctt tatgagattc cattccgacc tgagccaaag cttccagtgc gatgtcatga
      301 tccttcctcc ctggacgttt gaacaggcca agcgctccgt aacgacccat catcaccgtt
      361 tcgaggaccg tcacaggaaa atttcgatcg acgacccctt tctgcggaag gtagccgatt
      421 tttgcgcgat ggtggcagcg gagctcatca caggcgcagt cgaagatatg aagatggccc
      481 tccactggag cgagaagccc caggacggcg cggcaaagcg ttgtcttgcc tgatccgttt
      541 gggccgatga ctcctacgaa ctcgccctca tagatggtca acgaaatgtc ttggagcgcg
      601 atgagcccgg gaaatccgaa ggaggcatgg tcgaaacgga tgatcggttc gcggggatca
      661 gacacggaat ccgaagctcc ttccggcgcc aagatgagaa tagttacgag gtcgtttcca
      721 acgcaccggc caattggagc acatcatagc gaagcatgtc gaggtaggtc tcggttcccg
      781 ccaaaccgcc cggcatcgcc gtcaagacga cgacgcgggc cttggtctcc ctcgccagca
      841 gctccgggag tcgccggctg agttgaacct cggatacgac aaccttgatg ttttcctttc
      901 taatcttctc aatgagaaat tgcagatgat aggcggatgg ttctgtaccg gactgcctat
      961 gaatcgtgcc gacaatattg aagctgaatc gctttgccaa atagggccaa gccggatgat
     1021 gggcgatgaa acgccggtcg gacagccgct gagtccgctc gagcagttcc tgttgcacct
     1081 gacccagccg tcggaggtag gccgtttgat tggcctggta gtcagccgca tgacgtgggt
     1141 ccacttcgat gaaggcttgg gtgatatggc gcagcatgat ggggacattc tcagggtcca
     1201 gccagatatg cgggttcccg gcttcccctt catgatgcgt cccttcatga gagtcagcgt
     1261 cgtctcgaat cagcgctact ccttgggatg tcgtgatcac acgtaacgat gggtctcccg
     1321 cgttcttcac cagggatgaa acccagacct ccagtcccat gccgatctcc aacaacagcc
     1381 tagccttccg gactgcgacc agatcggtgg gtttgggtga ataggtgtgc tcgttttcgt
     1441 agccgctcaa caacgacgtg actcgtacat gaggaccacc gacctgctcc gccaaatcct
     1501 tcaggacggg aatcgtgacc accacaggaa tcgggtcgcg agcctctgta atggtgccga
     1561 aggagaggag gaaacctgcc agcacggcaa ggagagagaa gacaggcgac ttccgcacgt
     1621 ggttggccgg aaacatcggc ggggacggta tcatccgcgt attccggtgt caacgacatc
     1681 aggaaacgac aggatgccag gagatcaccg ttcaaaagaa acgtcctcag gatgttcaaa
     1741 aagtccgccc agcaaggccg cagcgagcga agaggcgaag cgtactttgg gccgtatgtt
     1801 gagccctcta agcgacgcga gaacgccgct ggcggacttt ttcaacatcc tgttaggccc
     1861 tgtccattcg cctccttgac cacacgccaa agttcgatta gcgtagcaca cctgtgaacg
     1921 tctcgggatt cgcgcgggtg gtggaaagga taccggcgat gaaggttgtc ggattgatgt
     1981 caggaacttc agccgatgga gtcgatgcgg cgctggtcac catcgcccgt caaagggcgg
     2041 gcatccgcgt cggaatgctc gcgtttcatt cgcttcccta cccgcgttcg ctacagcaac
     2101 gaattctttc cgcatcggtt tccggaacgg tgacggatat ctgccacctc aatgcgctcc
     2161 tgggcgaatg gttcgccgac gccgccctag gagttattcg gtcggctcaa ttatccccgg
     2221 aagacataga cctgatcgga tctcacggtc aaacggtgca ccacctgccg catggcatca
     2281 aagatacgcg cgtcggcgca atacgctcca cattgcaaat tgccgagcct gcggtcattg
     2341 ccgaacggac ggggattacc acggtggccg atttccgccc gcgcgacatt gcggcgggcg
     2401 ggcaaggggc gccgcttacc ccagggagcc atgccctgct gtttcgacat ccgcgccgcg
     2461 cccgactcat cgtgaatctt gggggcatca gcaacgtcac ctaccttccc cgaggatcag
     2521 gctctgagaa ccttgtcgcg ttcgatacgg ggccggcaaa tatggttctc gatgggctca
     2581 tgtctcgcac cacgaatggt cgagcctcga tggaccgaga tggacgtttg gcggccaaag
     2641 gcctggtgga ttcaagattg ctggccaaac ttctcgcaca tccgtatctg tcgcaggcgc
     2701 cccccaaatc gaccggtcgc gaggcgttcg gcgcaaagat gctggacgaa ttactcaatt
     2761 ggcagcaaac acgtcgggta tcggtcgaag atcttctggc cacctgtagc cgctggacgg
     2821 ccgaagcggt cggtacggcc aggcggtgga tcaggggagg aatcgatgac gtgatcgtgg
     2881 gtggaggcgg tgtccggaac cgggcgatca tgggacattt aaccgacatc tttgctccgg
     2941 tgtcggtcgc gccgtttgag tcgcacggtt gggacagtaa ggctctggaa tcggtggcgt
     3001 ttgcagtcct ggcgtatcaa accgtgatgg aacagtgcgg gaacgtccca tcggttacgg
     3061 gggcggcgtc cccaagattg ttggggtgca tcgtcccaag cggcccacgg tggtatgaac
     3121 ggctgcgctc gcgcaaaggc ggcagtaaga caagatgaaa attcttttgg ccggtgcggt
     3181 catcgccgtg agcatcggtc tttggggcta tctgaggagg gtgcgtcgaa gaaagaaaca
     3241 ggcatcgccg tttgccgttc ctcacgacgc caccatcagt tccgtccgat tgctcgctca
     3301 agaggaggtc gcattgtata gcctcctgca aatggccgtc caagagcgct atctcgtgtt
     3361 cagtcaggtg ccgctctggt ccttcgtcga ggtcgaagcg gcagcacagg tgcgttccga
     3421 agttttgcgc cagattgcgc tcaaacgagt cgattttgtc ttggtccacc ccgggtcgtg
     3481 cctcgtcgaa caggtcgtgc agatcgagcg tgaatcttcg caacctcatg aagctgagcg
     3541 gcagcacgtc atcgagtcag tgctcgatgc tgcggggatc aagctgacga agttgcagcc
     3601 gaagcaaacc cattcgttgc ccgatctcat cgctagactg ggattagtcg aagaggaata
     3661 acgacgaagg cttcggatgc aatgtgagaa gccatctagc aattcagaac caggggctcc
     3721 attcgccact cggtgatttt tgtgaacgta atagacggaa t
//
LOCUS       DATMTI010000197         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_729512, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000197 DATMTI010000000
VERSION     DATMTI010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729512"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="glmM"
                     /locus_tag="VN039_03295"
     CDS             <1..1263
                     /gene="glmM"
                     /locus_tag="VN039_03295"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWV45047.1"
                     /translation="AAAHLFMRRAGRHQIVIGKDTRISGYMLESALMAGICSMGVDVL
                     LVGPMPTPAIAFLTRSLRADAGVVISASHNPYQDNGIKFFSSDGFKLPDEVEARIEEL
                     IVSDEISHLRPTADLIGKAYRIDDAEGRYIEFAKRSLPKDLDFQGIKLVVDCANGAAY
                     KVAPTVLRELGATVEVIGNKPDGMNINAGCGAVHPELLQESVRRYKADLGIALDGDAD
                     RAVFVCEQGTVIDGDHVMAALGLDLHRNGLLAKQTLVGTVMSNFGLELSMLKAGVKLI
                     RTPVGDRYLLERMLGEGYNFGGEQSGHFIFLDHNTTGDGLISALQMLSLVKRTKKPLS
                     ELAKAMTAVPQVLVNVHVTKKPRLESIPDIDCAMQESERRLNGCGRVLIRYSGTEPLL
                     RIMVEGEQSTMVEEVAEDLARVVRKHIG"
     gene            1299..>2152
                     /locus_tag="VN039_03300"
     CDS             1299..>2152
                     /locus_tag="VN039_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family competence protein"
                     /protein_id="HWV45048.1"
                     /translation="MLPSLTAVFVLGLLCGAQISFFPLSVIALLAGIAVGFSILERVG
                     YIDSPSALLLYSSLLSGLGYWTLTTPTLEFSRMSPPLHETVQVSVVGRVIAPVQYSVG
                     RQTIMLQTDESDSASRRIRLVWRDPGIMLHHGDRISFRGKLHRPRGSLNPGGFDYASY
                     MDRQGIDFLTTVTGAQAVTLLDAEPQIGLWSFWNRIDHWRAMIRAAAISTLSQPTRGL
                     FLGMITGERGYLEQELQDWFMVTGTVHLLSISGSHLGLVAIVVFWLVKRLVLVLPLTL
                     LLTLSRIAT"
BASE COUNT          458 a          553 c          612 g          529 t
ORIGIN      
        1 gccgccgcac atctctttat gcgacgagcg ggccgccatc aaattgtgat cggtaaagac
       61 actcgtatct cgggatatat gctggaatcc gctttgatgg cggggatctg ttcgatgggg
      121 gtcgatgtcc tgttagtcgg accgatgccg acaccggcga tcgccttttt gactaggagt
      181 ctacgggcgg atgcaggagt agtcatttca gcatcgcaca acccttacca agacaacgga
      241 atcaaattct tttcaagcga cggattcaag cttcccgacg aagtggaagc tcgcattgaa
      301 gaactgatcg tatcggatga aatcagtcat cttcgaccga cggcagacct catcggcaaa
      361 gcctaccgta tcgacgatgc agaggggcgt tatattgaat ttgcaaagcg gtcgttgccg
      421 aaggatttgg attttcaggg aatcaagctc gtcgttgatt gtgcaaatgg tgcggcatat
      481 aaagtcgctc cgacggttct cagggaattg ggcgccacgg ttgaagtcat cggcaataaa
      541 ccggacggga tgaatatcaa tgccggttgc ggcgccgtcc atcctgaact gctgcaagag
      601 tcggtacgcc gttacaaagc ggatcttggt attgccttgg acggcgatgc cgatcgggcc
      661 gtgtttgtct gtgagcaagg aacggtcatc gatggcgatc atgtgatggc ggccttgggt
      721 ctggatcttc atcgaaacgg actcctagcc aagcagacct tggtcggaac cgtgatgagc
      781 aattttggac tggagctgtc gatgttgaaa gcaggcgtta agctgattcg gacgccggtc
      841 ggtgatcggt atctgctcga acgaatgttg ggtgaaggct ataacttcgg aggagaacaa
      901 tcgggccact tcatattcct tgaccacaat acaaccggag acggcctcat ctcggctctg
      961 cagatgttat cattggtgaa gcgaaccaag aagccgttat ccgagttggc caaggcgatg
     1021 accgccgtcc cgcaagtgtt ggtgaatgtg catgtcacga agaagccgcg attggaatcg
     1081 attcccgaca ttgattgcgc catgcaggag agcgaacgtc gtctgaacgg gtgtgggcga
     1141 gtcctcatca gatattccgg gaccgagccg ctgttgcgga tcatggtgga aggagaacag
     1201 tccaccatgg tcgaggaagt ggctgaggac cttgccaggg ttgtacgaaa acatatcggc
     1261 tgagcgttcc ctcttcctcc ggtccatgag ggaagctcat gctcccatcc ctcacagcag
     1321 tgtttgtcct cggtcttctg tgtggggccc agatctcctt tttcccgctc tctgtcatag
     1381 ctctgctggc cggcatcgcc gttggattca gcattctcga gcgggtcggt tatatcgact
     1441 caccctctgc actgcttctg tattccagcc ttctctccgg actaggttac tggactctca
     1501 ccacaccgac gttggaattt tcccggatgt cgccgccgct tcatgagact gttcaagtct
     1561 cggtcgtcgg tcgggttatc gctcctgttc agtacagtgt ggggcgtcag acgatcatgc
     1621 ttcaaacaga cgaatcggat tcggcatcga gacgcatacg ccttgtgtgg cgcgaccccg
     1681 gcatcatgct tcatcacggt gatcgtattt cgtttcgagg aaaacttcac cgtccacgag
     1741 ggtcgttgaa cccgggtgga ttcgactatg cttcctatat ggatcgtcag ggcatcgact
     1801 ttctgaccac ggtgaccggt gctcaggcgg tgacgctgtt agatgcagag cctcaaatcg
     1861 ggttgtggag tttctggaac cggatcgatc attggagggc catgatacgt gcagcggcta
     1921 tcagcacatt gagccaaccc acccgagggc tatttctggg catgatcacc ggtgaacgag
     1981 gctatctcga acaagagctc caagactggt tcatggtgac cggaacagtt catctgctct
     2041 cgatctccgg ttcacatctc gggctcgtgg ccatcgtagt tttttggctg gtgaaaagac
     2101 tcgttcttgt gcttcccctc actctcttat tgacgctatc acgaatagcc ac
//
LOCUS       DATMTI010000198         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_135673, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000198 DATMTI010000000
VERSION     DATMTI010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135673"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..266)
                     /locus_tag="VN039_03305"
     CDS             complement(102..266)
                     /locus_tag="VN039_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45049.1"
                     /translation="MPKAQAMEIAAKSKDKETRKAHRIGITYAYMNDDNRVPPAKMAE
                     LAKHACENPE"
     gene            complement(259..459)
                     /locus_tag="VN039_03310"
     CDS             complement(259..459)
                     /locus_tag="VN039_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45050.1"
                     /translation="MIEWIEANDIWFHFALFVLIALWVEIRLERYMDKLEDSYVRDRD
                     ELLYGSKSFHHGRGSEETSRDA"
     gene            complement(621..809)
                     /locus_tag="VN039_03315"
     CDS             complement(621..809)
                     /locus_tag="VN039_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45051.1"
                     /translation="MSQSKPVPHTLSAVYKTDKGARMNHGPVQYPTRAEAERQKRILR
                     ADILKSGGQFLASVIERA"
     gene            complement(878..1261)
                     /locus_tag="VN039_03320"
     CDS             complement(878..1261)
                     /locus_tag="VN039_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45052.1"
                     /translation="MDDGKHSHYKKDVEHLHMVDVYRVLALYDVTDPALQHAIKKLLV
                     AGGRGAGKDVSQDIQEAIDSLERWKDMQMENELRGRLVLNTVITKEVRCEKGILRSVM
                     PEGIPVRRTNKGRAEAARKRKGKSR"
     gene            complement(1254..1502)
                     /locus_tag="VN039_03325"
     CDS             complement(1254..1502)
                     /locus_tag="VN039_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45053.1"
                     /translation="MDAFDRGLHELLEDELSGDSLHYEQPRQYNPPHCEQCGARCMWF
                     EVQGKYILHDPVTGDPHRCNFGVTIDLEGLKPYGDGNG"
     gene            complement(1563..1736)
                     /locus_tag="VN039_03330"
     CDS             complement(1563..1736)
                     /locus_tag="VN039_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45054.1"
                     /translation="MITQPKTQMKYVSGETLYEIYREHANLRVKDLPFWCNLKGEARD
                     MWMRIAEELNSRR"
     gene            complement(1825..2217)
                     /locus_tag="VN039_03335"
     CDS             complement(1825..2217)
                     /locus_tag="VN039_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45055.1"
                     /translation="MELDQRYVVLKIKDLDNAISHEDGQTLNALCKKCESYRKARGAA
                     PLHGLFIERDWPEHGLAMTALENRIGGEEKAEYSLFVSDELEANVYGVTEEAKREIMG
                     YARKYSEHGATIRIERVYRHDVTAEFGM"
     gene            complement(2220..2450)
                     /locus_tag="VN039_03340"
     CDS             complement(2220..2450)
                     /locus_tag="VN039_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45056.1"
                     /translation="MSLNDRFILEAFVFAKEQDGFAKSCRVHPDTRSEIIASADFCRN
                     HWPLQGEPATYQGIVLVIDKAVPVGDFVFSLE"
     gene            complement(2447..2656)
                     /locus_tag="VN039_03345"
     CDS             complement(2447..2656)
                     /locus_tag="VN039_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45057.1"
                     /translation="MIPPGYVSELERLENLRARCGNRFPTACWTGNCRPFVDKRGRLH
                     DTLGKIVIPLKPRFMIVRKGKRKAK"
     gene            complement(2656..2862)
                     /locus_tag="VN039_03350"
     CDS             complement(2656..2862)
                     /locus_tag="VN039_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45058.1"
                     /translation="MSPSITTQFNVLMQSGCSYTSEGVQGLLRADKDLAAKVALLPES
                     AREGRLPEGSYETIVDTIQGAEPL"
     gene            complement(2958..>3227)
                     /locus_tag="VN039_03355"
     CDS             complement(2958..>3227)
                     /locus_tag="VN039_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45059.1"
                     /translation="LSAMYREIRTMSTLSPNISSDFYNVGVKFVGPRGVRLKHDKTLK
                     ETARTYLKKKPRHFLTREAADAYLKTLPAEVQAICEVYEFCYAGI"
BASE COUNT          625 a         1007 c          828 g          767 t
ORIGIN      
        1 gtcagcagac tctgcttcgt ctgccgcttc cttcagaacg cgttgcgcgt ccttcagctt
       61 gttgatgacg acttccagtt tctctgatct cgtgaaaaaa ctcattccgg gttctcacag
      121 gcgtgtttgg ccagttccgc catctttgca ggcggaacgc gattgtcgtc gttcatgtat
      181 gcgtaagtga tgcctatccg atgggccttg cgcgtctctt tgtctttgct cttcgccgcg
      241 atctccatcg cttgcgcttt aggcatccct gctcgtttct tcgcttccgc gtccatggtg
      301 aaatgactta ctgccataca gaagctcatc acggtcgcga acataagaat cctccagctt
      361 gtccatgtag cgttccaggc gaatctcgac ccacagcgca atcagaacga acagagcgaa
      421 atgaaaccag atgtcgttcg cctcgatcca ttcaatcaca atggctctcc cagattgcgc
      481 gcagctttcc agacgcattc gcccaaagcg acgtcccgcg cagcgatgtc accatcgtac
      541 cgcgcaatcc agacgtcttg ttctttgcgg aagataacgc ctaagtcttt cagacgatac
      601 cagagcgcag tcgttcccac ctatgcgcgc tcgatgacgc ttgcgaggaa ctggccgccg
      661 ctcttcagaa tgtcagcgcg caagatgcgc ttctggcgtt ctgcttctgc ccttgtcgga
      721 tactgcacag gaccatgatt catgcgggcg cccttgtcgg tcttgtagac agcggacagc
      781 gtgtgcggaa cgggcttgct ctgcgacatc gtgaactcca tacgaatgta gaacgtgcgt
      841 ataccttacc gcacgttcta cgcgagtgca atgactttta gcggctcttg cccttccgct
      901 tacgcgccgc ttcagccctt cctttgttcg tgcgcctcac agggatgcct tccggcatca
      961 ccgaccgcag gatgcctttc tcgcagcgca cttccttcgt gatgacagtg ttcagcacga
     1021 gacgtccacg cagttcgttc tccatctgca tgtccttcca gcgctccagc gagtcgatcg
     1081 cctcctggat atcctggctc acgtccttgc cagcaccacg accgcctgcg acgagaagct
     1141 tcttgatcgc atgctgcaac gcagggtcgg tgacgtcgta cagcgccaga acacgataca
     1201 cgtccaccat gtgcaagtgt tcgacgtctt tcttgtagtg cgagtgcttt ccgtcatcca
     1261 ttgccgtctc catagggttt aagaccttca aggtcgattg tgacaccgaa attgcaccga
     1321 tgcgggtcac ccgtcaccgg gtcgtgcagg atgtacttgc cttgtacctc aaaccacatg
     1381 caccgcgcgc cacattgctc gcagtgcgga ggattgtact gccgaggctg ttcgtaatgg
     1441 agcgaatcgc cggaaagctc gtcttccagc agctcgtgca agccgcgatc aaacgcatcc
     1501 attgcgccat cacctgatgg atagtacgaa aacaggcacc gtctacctcg cgacatgacg
     1561 aattatctcc gactgttcag ttcttccgcg atgcgcatcc acatatcccg cgcttcgccc
     1621 ttcagattgc accagaaagg aagatccttc acgcgaaggt tcgcatgttc gcgatagatc
     1681 tcgtacaatg tctcgccgct cacatatttc atctgcgtct tcggttgagt aatcactatg
     1741 ccgtctcgct ttcgctgatt cgcaggctgc gcatattacc acgcgcagca cattgcccgc
     1801 gcgagcagcg cagaaagcac cgattcacat gccgaattct gcggtcacat cgtgacggta
     1861 aacgcgctca atgcgaatcg tcgcaccgtg ttccgaatac ttccgcgcgt agcccatgat
     1921 ttcgcgcttt gcttcttcgg tcacaccgta gacattcgct tccagctcgt cgctcacgaa
     1981 taggctatat tccgccttct cttcgccgcc gatgcggttc tcgagcgccg tcatggcgag
     2041 cccgtgctcc ggccaatccc gctcgatgaa caacccgtgt agcggtgctg caccgcgagc
     2101 cttccggtag ctctcgcact tcttgcagag ggcattcagc gtctgcccgt cctcgtgcga
     2161 gatcgcgttg tcaaggtcct tgatcttcag gacgacgtag cgttggtcaa gttccatggt
     2221 cactccaagc tgaaaacgaa gtcgccaaca ggcaccgcct tgtctatgac caagacgatg
     2281 ccctggtacg tcgcaggctc accctgcaag ggccagtgat tacggcagaa gtcggcactc
     2341 gcaatgatct cgctgcgcgt gtcaggatgc acccggcacg atttcgcgaa tccgtcctgc
     2401 tccttggcga acacgaatgc ctccagaatg aaccgatcgt tcaggctcat ttggccttcc
     2461 ttttgccctt ccgcacgatc atgaaccgtg gcttgagcgg aatcacgatc ttgccgagcg
     2521 tgtcgtggag cctgccgcgc ttgtccacga acggccggca gttacccgtc cagcatgccg
     2581 tcggaaagcg gttgccgcac cgcgcccgga gattctccag gcgctccagc tcgctcacgt
     2641 atccgggcgg gatcatcaca gcggctctgc tccttggatc gtgtcgacga tggtctcgta
     2701 cgaaccctcc ggcagtcgac cctcgcgggc ggactccggc agcagcgcca ccttggccgc
     2761 gaggtccttg tctgcgcgaa gcaggccttg gacgccctcg ctcgtgtacg agcaaccgct
     2821 ctgcatgagg acgttgaact gcgtggtaat gctcggactc atcttgtatc tccgcgttgt
     2881 gtggtaactg tgcatactat gcgctgacca cagtgtggag cgcaagacac ggctcgcaga
     2941 aagtcaagac tatttgctta gatacccgcg tagcagaact catacacttc gcagattgct
     3001 tgcacctccg caggaagcgt cttgaggtac gcatctgctg cttccctcgt caggaaatgc
     3061 ctgggcttct tcttgagata agtgcgagcc gtctccttta gcgtcttgtc atgcttcaga
     3121 cgaacgcctc gcggtccaac gaatttgacg cccacattgt agaagtcact ggagatgttt
     3181 ggagacagag tcgacatggt gcggatctct cgatacatcg cagaaag
//
LOCUS       DATMTI010000199         5775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_305721, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000199 DATMTI010000000
VERSION     DATMTI010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305721"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VN039_03360"
     CDS             complement(<1..477)
                     /locus_tag="VN039_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45060.1"
                     /translation="MSETKHETKTEVKDTAAAEAAAEAAKAAKAAEKAAKAEQKKADA
                     DKKKAEREVKKAEQAAAREQKKADELAKKEQAKADAAAAKEAARMPEQNGVRRPKPDT
                     LCGKAWLIFDQISQKNGAAASIGESLKVAEEQGLNVGNVRAEYARWRKFFGVSGRIS"
     gene            complement(578..1297)
                     /locus_tag="VN039_03365"
     CDS             complement(578..1297)
                     /locus_tag="VN039_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45061.1"
                     /translation="MWIAIDTKHHKFLNIRHLSMHVVADLVWLESRLQDVLICSDEWP
                     FLQGMNDIDMHILYKNTSGQAAKYVGDNLRAVLMHMAQQIPERDVNPVELDAQIRTLN
                     EEEYIPRRYVKGVMVPETLPKGTLGSFSAPILLTDEAAIVRLPRALPQVSERPRAAAT
                     PQANPSARPARAPSAPRQGGVREVIWAVADRMWEAAGSPKDAKAVLELRKQMMTTLEA
                     DHGVKRTSSSNELGQWMKARI"
     gene            complement(1297..2727)
                     /locus_tag="VN039_03370"
     CDS             complement(1297..2727)
                     /locus_tag="VN039_03370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWV45062.1"
                     /translation="MSRNWSQYQKDIFSFVETGKGNAIVVAVAGSGKSTTIVEALGRV
                     KGNAIFLAFNKAIAEELKARGVNARTFHSLTYGTVANHLGGAMDGDKLRKLVQTNFTN
                     GNDAFIYGAFCCRLVSLGKQAGVGCLINDTEQTWIDICAYHDLEPEHEDADFKRAIEL
                     ASDLLRYSNESRNFDFDDMLYIPVKEGLSLPKFDFIFVDEAQDTNAIQRALLKKLFKP
                     STRMVAVGDPAQAIYGFRGADSDSLHRIAKDFKAITLPLSVSYRCGQNIVKYARKWVS
                     HIEHAEGAHEGIVQEHTKWDLDIFQGNDLVVCRTTRPILALAFRLIKARIPAFVMGRE
                     IGQGLKALIRKLKAADVPDLLEKLGRWREREIEKALVKMEEAKIESINDKADCILVLC
                     DDIESIMDLNDAIDNVFANKENAVKLATIHKSKGLEAQRVFWLNRQACPSKWAKQKWQ
                     KEQEDNLCYVAATRAIAELHLIEEVV"
     gene            complement(2791..4539)
                     /locus_tag="VN039_03375"
     CDS             complement(2791..4539)
                     /locus_tag="VN039_03375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWV45063.1"
                     /translation="MQIKLRSYQSEAVDCHYAFFRENAKGNPIIAMPTGTGKSLVIAG
                     FLESIYKTWPNQKVLVITHVKELIRQNYLKLIALWPTAPAGIYSASLNRRDTLQKIVF
                     CGIASVAKRAHEFGHVDLVLIDEAHLVSPTEDTMYRKFLAELTNVNPHMRVIGYTATK
                     WRLGHGDITEGEDGIFTHTAFDITGIESFNRLIAEGYLAPLVPKKTNFLLEVDGLHMR
                     GGEFIPAELQTAFNKDEITERALREVIETCGDRKKWLIFAAGVEHAVSIAELLDHMGI
                     PCAAVHSQISDKERDQALRDLHTGKLRAVVNNNILTTGYDEPGIDLIIVLRPTASSVL
                     WVQMLGRGTRPCPDEGKIDCRVMDFAGNTRRLGPINDPVIPRKKGMKGGTAPVKECPM
                     CNTLVHASLRFCNGTKWDDTKCEYEFPIQTKITMEASTEDVVKGDLPVVERFPVEHIT
                     FSLYKKEGKPDSIRVTYYSGLKNYSEYVCLEHAGYASHKARQWMRERMPDKSLEAPAA
                     TLRALEYASNLKVPSAIRVWTNRKYPEILSYEWEENDIAQVTIQGVDHSRMAPPRQMF
                     EVPQPENAYLDDDIPF"
     gene            complement(4529..5233)
                     /locus_tag="VN039_03380"
     CDS             complement(4529..5233)
                     /locus_tag="VN039_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45064.1"
                     /translation="MLLTIGCHVWQQDENGKQFRISGSDGHFGGSGDGIIGSLPDLDH
                     NQVALGEFKTHNEKSFHKLAGKNWNKYVDWLVGREKDAQKFEGEGVRKAKFEHYVQMQ
                     IYMRKMGLSVALYVAVDKNSDGIYAELVPLDTEIADQFIERADKIVWLQKAPRRIATS
                     ASDWRCMWCDHKPVCYGKAAPDRNCRTCIFSSPQPGGNGRWACSQPDVRCDDLSAALQ
                     YTGCAHYVARSDFSAD"
     gene            complement(5524..>5775)
                     /locus_tag="VN039_03385"
     CDS             complement(5524..>5775)
                     /locus_tag="VN039_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45065.1"
                     /translation="GSLGALSFGGVEMADDADNADVREAVELQAKEMNIRAAAARFDK
                     GAPGECYFCGEEFSRVVSVLWQSENVQSCGRCRDKRGIR"
BASE COUNT         1119 a         1701 c         1562 g         1393 t
ORIGIN      
        1 ggagatgcga ccggacacgc cgaagaactt gcgccagcga gcgtactcgg cgcgcacgtt
       61 gcccacgttc aggccctgct cctcggcgac cttcagcgac tcgccgatgg acgcagcggc
      121 accgttcttc tggctgatct ggtcgaagat gagccaggcc ttgccgcaga gcgtgtcggg
      181 cttcggacga cggacgccgt tctgctcggg catgcgcgcc gcttccttgg cagcagccgc
      241 gtcagccttc gcctgttcct tcttggccag ctcgtcggcc ttcttctgct cgcgcgcggc
      301 cgcctgttcg gccttcttga cctcgcgctc ggccttcttc ttgtcggcgt cggccttctt
      361 ctgctcggcc ttcgcagcct tctcggcagc cttcgcggcc ttggcagctt ccgcagcggc
      421 ttcggcggca gcggtgtcct tgacttcggt cttggtctcg tgcttggtct cgctcatgac
      481 gtgtactccg tggttgagtt tgtggaaagt gttgcgcccg ctgatggacg ggagttgcaa
      541 ggtactgcaa gcggccgctg aatgcaagca ttatttttca gatacgagct ttcatccatt
      601 ggccgagttc gttgctgcta cttgtccgtt tgacaccgtg gtcggcttcc agtgtggtca
      661 tcatctgctt gcgcagttcc agcactgcct ttgcgtcttt cggactgcct gcggcttccc
      721 acatgcggtc cgcaactgcc cagatcacct cgcgcacgcc accctggcgt ggggcgctgg
      781 gggcacgcgc aggccgcgcg cttgggttcg cctgtggggt agcggcggca cgggggcgct
      841 cgcttacctg cggcagcgca cgcggtaagc gcacgatcgc agcttcgtcc gtaagcagta
      901 tcggcgcgga gaaagaaccg agtgttcctt tgggcagagt ttcgggcacc atgacgcctt
      961 tgacgtaacg acgagggatg tattcttcct cgttcagcgt tcgtatctga gcgtcgagct
     1021 ccaccgggtt tacatcccgt tcaggaatct gctgcgccat atgcatcaga acagcacgca
     1081 gattgtcgcc gacatacttg gcagcttggc cggacgtatt cttgtagagg atgtgcatgt
     1141 cgatgtcatt catcccttgc aagaacggcc actcgtcgga gcagatcaga acatcctgca
     1201 ggcgactttc gagccacaca agatccgcga cgacgtgcat gctcagatgg cggatattca
     1261 agaacttgtg gtgtttggta tcgatcgcga tccacatcac acaacctcct caatcagatg
     1321 aagctctgca atcgcgcggg ttgctgcgac ataacagagg ttatcctcct gttccttctg
     1381 ccacttctgt ttcgcccact tgctggggca ggcttgacgg tttagccaga agacacgctg
     1441 tgcctccagg cccttggact tgtggatggt cgccaacttt accgcgttct ctttgttggc
     1501 gaagacgttg tcgatcgcat cgttgagatc catgatcgat tcgatatcgt cgcacaagac
     1561 cagaatgcaa tccgccttgt cgttgatact ctcgatcttg gcctcttcca tcttgacgag
     1621 cgccttttcg atctcgcgtt cgcgccaccg acccagcttc tccagcagat ccggaacgtc
     1681 agccgccttc aacttgcgga tcaacgcctt gagaccttgt ccgatctccc tccccatgac
     1741 gaaggccggg atgcgagcct tgatcaatcg gaaggctagt gcaaggatcg gtcgcgtagt
     1801 gcgacatacg acgagatcgt taccttggaa gatatccaag tcccacttcg tgtgttcctg
     1861 aacgattcct tcgtgcgccc cttccgcgtg ctcgatgtgg ctgacccatt tacgcgcgta
     1921 cttgacgata ttctgaccac agcgatagct gacggacagc gggagcgtga tcgccttgaa
     1981 gtccttcgca atgcggtgca acgaatcgct atccgcaccc cggaagccat agatcgcttg
     2041 cgcagggtcg cccaccgcaa ccatgcgcgt tgagggcttg aagagtttct tgaggagcgc
     2101 gcgttggatc gcgttcgtat cctgcgcttc gtccacaaag atgaaatcga acttggggag
     2161 agacaagcct tccttcaccg ggatgtagag catgtcgtcg aaatcgaagt tccgcgactc
     2221 gttgctatag cgcagcaagt cagatgccag ctcgatcgcg cgcttgaaat cggcgtcttc
     2281 gtgttccggt tccagatcgt gatacgcgca gatgtcgatc caggtctgct cggtgtcgtt
     2341 gatcaggcag cccacacctg cttgcttgcc taggctcacc agcctgcagc agaacgcacc
     2401 gtagatgaat gcgtcattgc cattcgtgaa gttcgtctgg acgagcttgc gcagcttgtc
     2461 gccgtccatc gctccgccaa ggtgattcgc taccgtgccg tacgtgagcg aatggaacgt
     2521 gcgtgcgttg acaccgcgcg ccttgagttc ctcggcaatc gctttgttga aggccagaaa
     2581 gatcgcattg cctttcacgc gacccagcgc ttcaacgatc gtggtcgatt tgcccgaccc
     2641 tgcgaccgcc acgacgatgg catttccttt gcccgtctcg acgaaagaaa agatgtcctt
     2701 ctgatactgc gaccaattcc ggctcatgtg ggcggttccc gttaagttgt gcaaactata
     2761 acaggcgtta gcgctcaata caatgacttt ttagaacggt atgtcatcgt ccagataggc
     2821 gttctccggc tgcggtactt cgaacatttg ccgcggaggc gccatcctac tgtggtcaac
     2881 gccttggatc gtcacttgcg cgatgtcgtt ctcttcccac tcgtacgaaa ggatctctgg
     2941 atacttgcga ttcgtccaga cacgtattgc ggatggaact ttgagattcg acgcatattc
     3001 cagagctcgc aaggtggcgg caggagcttc gagactcttg tcaggcatgc gttcgcgcat
     3061 ccactgccga gccttgtgcg aagcatagcc cgcatgctcc agacagacat actccgaata
     3121 attcttgaga cctgaataat acgtgactcg tatgctatcc ggtttgcctt ctttcttata
     3181 aagcgagaaa gtgatatgct caacaggaaa tcgctccaca accgggaggt cgcctttgac
     3241 gacatcctcg gttgaggcct ccattgtgat cttggtctgg atcggaaatt cgtattcgca
     3301 cttcgtgtcg tcccatttcg ttccgttgca gaaacgcaga gacgcatgga cgagcgtatt
     3361 gcacatcggg cattctttga cgggagcagt tccgcctttc attcctttct tgcgaggtat
     3421 gaccggatcg ttgatcggcc cgagtcgtcg ggtgttcccc gcgaaatcca ttacccggca
     3481 atcgatcttt ccctcatcgg ggcaggggcg tgttccgcgt cccagcatct gcacccatag
     3541 cacagagctg gccgtcgggc gcaacacgat aatcagatca ataccaggct cgtcatagcc
     3601 agtcgtgaga atgttgttgt tgaccacagc gcgcagcttg ccggtgtgca ggtcgcgcag
     3661 ggcttgatcg cgttctttgt cggatatctg cgagtgaacc gctgcgcagg gaatccccat
     3721 gtggtccaac agctcagcga tactgacagc gtgctccaca ccggccgcga agatcagcca
     3781 ctttttccga tccccgcatg tctcgataac ttcccgaagt gcgcgctccg taatctcgtc
     3841 cttgttgaat gcagtctgaa gctctgcggg aatgaattcg cctccccgca tgtgcagacc
     3901 gtctacttcc agaaggaagt tcgtcttctt ggggacgagt ggtgcaaggt aaccctctgc
     3961 aataagtctg ttgaacgact caatgcctgt gatatcgaaa gcagtgtggg taaagatccc
     4021 atcctcgcct tccgtgatgt cgccgtgccc caggcgccac ttggtcgccg tgtagccgat
     4081 cacgcgcatg tgcgggttga cgtttgtcaa ttccgccaag aatttgcgat acatggtgtc
     4141 ttcagtcggc gacaccagat gtgcttcgtc aatcagaaca agatccacat ggccaaactc
     4201 atgcgcacgc tttgcaacgg aggcgatgcc gcagaatacg atcttttgga gtgtatctcg
     4261 cctgttaagc gacgcactgt atataccggc tggtgcggtt ggccagagtg caatcagctt
     4321 cagatagttc tgccggataa gttccttcac atgcgtgatg accagaacct tctggttcgg
     4381 ccacgtcttg tagatactct cgaggaaccc cgcaatgaca aggctcttgc ccgtgccggt
     4441 gggcatggca atgatgggat tgccttttgc gttctcacga aagaacgcat agtgacaatc
     4501 gactgcttcg gattgatagc ttcgaagctt aatctgcact gaaatcgctc cgcgccacat
     4561 agtgggcgca cccggtatat tgtagcgcag ccgataggtc atcgcaacga acatcaggct
     4621 gagaacacgc ccagcgaccg ttgcctccag gctgcggact gctgaagatg caagtgcggc
     4681 agtttctgtc aggcgcagct ttgccatagc agacaggttt gtggtcgcac cacatgcacc
     4741 gccaatcaga ggcgctcgtt gcgatgcgcc gaggcgcctt ctgcagccag acaatcttgt
     4801 ctgcacgctc gatgaactgg tcagctatct ctgtgtcaag tggaacgagc tcagcataga
     4861 taccatccga gttcttgtcg actgcaacgt acagcgcaac gctgaggccc atcttgcgca
     4921 tgtagatctg catctgcacg taatgctcga acttggcctt gcggacgcct tcaccttcga
     4981 acttctgtgc gtctttctcg cgacctacca gccagtcgac gtatttgttc cagttcttgc
     5041 ctgcgagctt atggaaggat ttctcgttgt gcgttttgaa ctcgccaagg gcgacctgat
     5101 tgtggtcaag gtcaggaagg gagccgatta tgccatctcc agaaccgccg aagtgcccgt
     5161 cggacccgct gatgcgaaac tgctttccgt tttcatcttg ctgccacaca tgacagccga
     5221 ttgtcagaag caacgcgatg aagcgccctt cttcaaggtg accacgattg aacagtctca
     5281 ggattcttcc actgaactgt cctttggtac accatctgtg actcatgtag attgcgcgag
     5341 cgcattcgcc gcccatgatg gacgcgccta agtgcgtgcg atgcgattcc tcttccgttc
     5401 tgtaagcgtc ccctatgtgg ggcagaaccc gtccgagcca tccgcggtat gctgctccgc
     5461 catcccgatg aaccatctct tcgaaattgc ggagggtctg caaagccaat cggacattcg
     5521 acattatctg atcccacgtt tatcgcggca acggccgcaa gattgaacgt tctcgctctg
     5581 ccagaggacg ctaacgactc tgctgaactc ttcgccgcag aaatagcact ctccaggcgc
     5641 acctttgtca aaacgcgctg cggcagcgcg aatgttcatt tccttggctt gcaactcgac
     5701 tgcttcacgc acatcggcgt tatccgcatc atctgccatt tccacccctc cgaaagaaag
     5761 cgcacccagc gaacc
//
LOCUS       DATMTI010000200         1801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_729764, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000200 DATMTI010000000
VERSION     DATMTI010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729764"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /gene="coaD"
                     /locus_tag="VN039_03390"
     CDS             <1..201
                     /gene="coaD"
                     /locus_tag="VN039_03390"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Catalyzes the conversion of ATP and pantetheine
                     4'-phosphate to diphosphate and 3'-dephospho-coA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWV45066.1"
                     /translation="HEFQMALVNRKLAKMVETVFLMPSEEYSYLSSTIIKDVAQHGGS
                     VTDFLHPEVARRLQARIRSLKS"
     gene            198..1391
                     /locus_tag="VN039_03395"
     CDS             198..1391
                     /locus_tag="VN039_03395"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWV45067.1"
                     /translation="MKLAARVGRIAPSPTLAMAATAKAMAAQGLDVIDFSAGEPDFDT
                     PDPVKAAAEAAIREGFTKYTPSSGIDELRSAIADKLQAQLGVRYEKSQILVSCGAKHS
                     LYNLAEALLEAGDEIIIPIPYWVSYSDQTLLNDATPVLLPTSEEDGYAVHASELERLI
                     TPRTKAIIVNSPCNPTGTTYDRATLEQIASAAVRHDLLVISDEIYEKVLYDGARHISI
                     ASLGPEIAARTVIINGVSKSYAMTGWRIGYAAGPKDLVTAMANIQSQSTSNPCSISQK
                     AAVAALRLGEPFTRKMVEEFSRRRIAIVDGLNKIPGVSCRMPTGAFYAFPNIKGVLGK
                     RGPSGVLRTPNDVAHYLLHDARIAAVPGEPFGSQEHLRLSYATSMANIARGLERLEQA
                     FAKLL"
BASE COUNT          431 a          484 c          501 g          385 t
ORIGIN      
        1 catgaattcc agatggcgct cgtcaaccga aaacttgcga agatggtgga gaccgtgttc
       61 ttgatgccca gtgaagagta ctcctacttg tcctcgacga ttatcaaaga cgtggcacaa
      121 cacggtggaa gtgtaactga ctttcttcat ccggaagtag cccgacgtct acaagctcgt
      181 atccggagtc tcaaatcatg aaattggcgg cacgtgtcgg tcgtattgca ccctctccaa
      241 cgctggctat ggctgcaacc gccaaggcca tggccgcgca aggattggac gtcatcgact
      301 tttcagccgg agaaccggat tttgacaccc cggatccggt gaaggccgcg gcagaagcag
      361 ccatacgcga gggttttacc aaatacacgc cgtcgtccgg gatcgatgag ttgcggagtg
      421 caatcgccga caagctccag gcccagctgg gagttcggta tgaaaagtct caaatcctcg
      481 tctcctgcgg ggcaaaacac tcgctctaca atttggcgga agccttactc gaagcgggtg
      541 acgagattat cataccgatc ccctactggg tttcctattc ggatcagacg cttctcaacg
      601 atgcgacgcc ggttctgcta ccgaccagcg aggaagacgg ttatgcagtc catgcgtcag
      661 agttggaacg cctcattacg ccgagaacca aagccattat cgtgaacagc ccgtgcaatc
      721 ctaccggcac aacctatgat cgggcgacgc tggaacagat tgcttcagcg gcggttcggc
      781 acgatctcct tgtcatttcg gacgagattt atgaaaaggt gctctacgac ggagccaggc
      841 acatcagcat tgcctcgttg ggacctgaga tcgctgcacg aacagtcatt atcaacggcg
      901 tatcaaagtc gtatgccatg acaggatggc gcatcggata tgcggccggt ccaaaagatc
      961 tggtgacggc gatggccaat atccagagtc agagcacctc aaatccctgc tcaatctcgc
     1021 agaaggccgc cgtcgctgcg ttgcgccttg gagaaccctt cactcgtaaa atggtggaag
     1081 aattttcccg tcgccggatt gccattgtgg acggattgaa caagattccg ggtgtgtcat
     1141 gccgaatgcc gaccggcgct ttctatgcgt ttccgaatat caaaggcgtg ttggggaaac
     1201 gtggcccctc gggtgtactc aggactccga atgacgtggc gcattatctc ttgcatgatg
     1261 cccgaatcgc ggccgttccc ggtgaaccct ttggaagcca ggaacatctc cggctgtcct
     1321 atgccaccag tatggcgaac attgcccgag gacttgagcg tctggagcaa gcgttcgcga
     1381 agttgctgta aggaaggatt gtctcatcga gctctatgca aaggtaacga gggaggaacg
     1441 cggtgccgat ttacgaatat cagtgcgaca gttgctcgta tcgttttgaa gtgaagcaaa
     1501 gcatcaaaga tgatccgctc tcgacgtgtg aacggtgtgg gagcgcggtc agacggctga
     1561 tttcatcgcc tgggatcatg tttaagggga gtggctggta cgtgaccgac tactccgata
     1621 aactgaaacc cccatctagc gtgagcagtg agactggcgg aacgagtact ggagaaaagg
     1681 gagaaaagaa ggagtctgcc gctctcgcta cacccagcgc aacatctcca gttcctgtat
     1741 cggctcctgc agcaacgcca accagttcat cgacgagcac accgagtatg acaacgccct
     1801 c
//
LOCUS       DATMTI010000201         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_772233, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000201 DATMTI010000000
VERSION     DATMTI010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772233"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VN039_03400"
     rRNA            complement(<1..824)
                     /locus_tag="VN039_03400"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1191..1266)
                     /locus_tag="VN039_03405"
     tRNA            complement(1191..1266)
                     /locus_tag="VN039_03405"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1231..1233),aa:Ala,seq:tgc)
     gene            complement(1322..1398)
                     /locus_tag="VN039_03410"
     tRNA            complement(1322..1398)
                     /locus_tag="VN039_03410"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1362..1364),aa:Ile,seq:gat)
     gene            complement(1570..>1702)
                     /locus_tag="VN039_03415"
     rRNA            complement(1570..>1702)
                     /locus_tag="VN039_03415"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          347 a          527 c          418 g          410 t
ORIGIN      
        1 tcccggcttg attggccttt cacccctaaa cacaactcat ccggtacctt ttcaacggta
       61 atcggttcga gcctccagtg cgtgttaccg caccttcact ctggtcatgc ctagatcgcc
      121 gggtttcggg tctaatgcat cgaactcatg gtcgccctat tcagactcgc tttcgctgcg
      181 cctacaccta acggcttaag cttgctcgat acattaagtc actgacccat tatgcaagag
      241 gtacgcggtc acatctcaag gatgctccca ctgcttgtag gcaatccgtt tcaggtactg
      301 tttcactccc ctcatcgggg tgcttttcac ctttccctca cggtactagt tcgctatcgg
      361 tcatgtacga gtatttaggc ttagagggtg gtccccctat attcagacag gattacacgt
      421 gtcccgccct actcaagtcc tgatacatca ctttcgcata cggggctgtc acccgctatg
      481 gcgctacttt ccagaagctt ctgctagttg aatatcaggc actggcctgg tccgcgttcg
      541 ctcgccacta ctaacggaat ctcggttgat gtcttttcct ccagctactg agatgtttca
      601 gttcgccggg ttcgcttcac gaaacctatg tattcagttt catgatactc ggaccccaat
      661 tacctagccg aagctagata atcgggatga gtgggtttcc ccattcggaa atctgcgggt
      721 caaaggttgc tcacacctca ccgcagctta tcgcagcgtg ccacgtcctt catcgcctgt
      781 acatgccaag gcatccacga attgccctta cctcacgctt gagagtccac accaccaacg
      841 acagcactgg gtgcatgcgt cagcgtgtgg aagttaatct cagccagata ttgtgattga
      901 accggcttgt cgtcttgcca cgccaccttg cggtgtcgcg ctcaactcaa agccgatacc
      961 catcacggca tcgattttaa gaacccattc acaatgtcaa agagagggcg aaccctcatt
     1021 gtcggcacaa ggccgaacct ctggtcttca tcatctggaa acttggttga gccactcccc
     1081 cgcgcgccgt cagtcgcagc aaagctgcgc cttgtggcgc gcttcgcggc gctggccgtg
     1141 cttgaaatgg tgcgcgccag cttcgctagt gcgtctccat ttctgcgcat tggtggagcc
     1201 tatcgggatc gaaccgatga cctcaagctt gcaaagctag cgctctccca actgagctaa
     1261 ggccccgaag gtttcgaaag ttccgggaaa cggacacgat cgcgagctgc ctgcctgcaa
     1321 gtggtgggcc gagtaggagt tgaacctacg acctcacgct tatcaggcgt gcgctctaac
     1381 cacctgagct accggccccc ttgcaccatc gccggccaat aggccgcggg cggcaagagc
     1441 cagctcaggc ttcatccccc aaggggatgt tccagtgatg aagggacatg aggacggcgg
     1501 ctatgttctt tggacaggag gaagctcttc cccaaccgta gtcgaggcgc tttccgccat
     1561 gatccttaga aaggaggtga tccagccgca ggttccccta cggctacctt gttacgactt
     1621 caccccagtc gctaagccca ccgtggtcgc ctgcctctct tgcgagttag cgcaacgcct
     1681 tcgggtgaac ccaactccca tg
//
LOCUS       DATMTI010000202         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_221354, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000202 DATMTI010000000
VERSION     DATMTI010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221354"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..2303
                     /locus_tag="VN039_03420"
     CDS             117..2303
                     /locus_tag="VN039_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45068.1"
                     /translation="MCGIAGFHVKDPSIIKKHEGVEIFVDELLKGIEHRGRHATGFVS
                     VGFDGRVALDKSPVTASDFIKDRERVPEGVRTVLLHTRHWTKGKPEHTENNHPVVFGT
                     CFAVHNGHINNDDELFEEWDLKRNAEVDSIIIPAAADFHGIGNPDDIKATLEALEGGF
                     ATAIINPVHNKGRVVIAKGKNSPLYVVDHPSFIVWASEKDVIKGAWGKVLGTPPNDKK
                     IEQIAQGTFIIVDDTEVTRESFTVKERPFVPRPNPLAPIQRQSGPKHGPSGSGFGFGR
                     HVQIDATPVAQAREAIQEFIKAGGTARTFDDRQNGLIDDDDLKDVKGAVAWHTCYGCN
                     RWILKEDIIEHELWGKVCIDCEAATKREIAKQEAIMQDTWEDDDVCAGTTVPRIAAAD
                     RDALEVWADHENSLHKETLEAMYQDLGSYYTTKAIEFLCFYTTPEYRDVAGAAVNALI
                     TELRELYEETYFDIVGESFPDEGDACAVPQESNDHPLLLEAGAQQRWRFCFTHNVTWK
                     SEEGGCHLCIKEDDENMADEAALAAILDRSGETEEATPTDAASTEVGVYLPWERGRDT
                     IITRSARTHRRSSGGTAEGEEGTGGSEDGDGEVIELPRCPICHTFVLRAGVDGCVVCR
                     ESAQDDGADVPGVGGGLKCIACLAYDAKVVIEYAGKSFHYCNTCYHTCNKLIHAEDKT
                     VTLCHNDTNHMLKDGTRVCHIHVRNQSGSVSDTTMRKRGASITEVK"
     gene            2306..3148
                     /locus_tag="VN039_03425"
     CDS             2306..3148
                     /locus_tag="VN039_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWV45069.1"
                     /translation="MFFLFHKTTRPTGRLLAKALGIRHFGSVVKAGIQPDFLIRWGNA
                     MPVAGRYTEVNSPAAISAASNKLRAFRKFHEAGMSIPKFTDSRYEAVDLQRQGYTIFG
                     RTSSGTKGRGIVIYRPGQEIGYHELYTGFIPNIREYRLHVVGDSVIRTQRKYPERAGA
                     TADAPIKNVEHGFVFKAPQRKLNKDREDLAVSAVKALGLDFGAVDMVIDENNVAYVLE
                     INTAPACSPMTGTAYVTALRELVLAKSNGGYDLATNLDALKGATASGEVDPRNRVMEA
                     IIGR"
BASE COUNT          774 a          858 c          981 g          629 t
ORIGIN      
        1 caccgaacta tcgaaagcaa gcaaaggcag agcgggtgca ggcgggagcg cgtcagtcat
       61 atgaccgctt cgtcgccgac tttgttgcca acaacgcaag gaggtaatga cacagcatgt
      121 gtggtattgc tgggttccac gtcaaagacc cctcgatcat caagaagcac gaaggtgtgg
      181 agattttcgt tgacgagttg ctgaaaggca tcgagcatcg aggtcgccat gccacgggct
      241 ttgtctcggt gggtttcgac ggacgcgtcg cactcgacaa gtcgccagta acggcgtctg
      301 acttcatcaa ggatcgtgag cgcgtgccgg agggtgttcg caccgttctg ctgcacacac
      361 gacactggac gaagggaaag ccggagcaca cggagaacaa ccatcccgta gtgttcggca
      421 cgtgcttcgc tgtgcacaac ggccacatca acaacgacga cgagttgttt gaggagtggg
      481 atctcaagcg taatgctgag gttgactcca tcatcatccc tgctgctgct gattttcatg
      541 gcatcggcaa tccggatgac atcaaagcaa ctctggaagc gttggaggga ggctttgcca
      601 cagcgatcat caacccggtg cacaacaaag gccgggtcgt gattgctaag ggcaagaatt
      661 ccccgctgta cgtggtggat cacccgagct tcatcgtgtg ggcttcggag aaggacgtta
      721 tcaagggcgc atggggcaag gtgcttggca ctccgcccaa tgacaagaag atcgaacaga
      781 tcgcccaggg tacgttcatc atcgtggacg acacggaggt tacgcgtgaa tcattcacgg
      841 tgaaggagcg ccctttcgtc cctcgcccca atcctctggc accgatccag aggcagagtg
      901 ggccgaagca tgggccgagt gggagcgggt tcggattcgg gaggcacgtg cagatcgacg
      961 caactcccgt cgcacaagca agagaggcca ttcaagagtt catcaaggcg ggcggcaccg
     1021 ctcgaacctt tgatgatcgg cagaacggtc tgatcgacga cgacgacctc aaggacgtca
     1081 aaggtgcagt tgcatggcac acctgctacg gctgcaatcg gtggattctg aaagaggata
     1141 tcatcgagca tgaattgtgg ggcaaggtgt gcatcgactg tgaggcagcc accaagcgtg
     1201 agattgccaa gcaggaagcg atcatgcagg acacatggga ggacgacgac gtgtgcgctg
     1261 gcaccaccgt ccctcgcatt gcagcagcgg atcgtgacgc gctcgaagtt tgggccgatc
     1321 acgagaactc actgcacaag gagacactgg aagcgatgta tcaggacttg ggttcgtact
     1381 acacgaccaa ggccatcgag ttcctgtgct tctacaccac gccggagtac cgcgatgttg
     1441 ccggtgcagc ggtgaacgcc cttatcacgg agcttcgtga actgtacgag gaaacctact
     1501 tcgatatcgt cggagagagt ttccccgacg agggtgatgc atgtgcggta ccgcaggaga
     1561 gcaacgacca cccgcttctg ttggaggcag gagcacagca acggtggagg ttttgtttca
     1621 cacacaacgt cacctggaag tccgaggaag gtggatgtca cctttgcatt aaggaagatg
     1681 acgagaacat ggctgacgag gctgcacttg ctgccattct ggatcgcagc ggcgaaacag
     1741 aggaagcaac gcctaccgat gccgcctcta ccgaagtcgg cgtctacctc ccgtgggaac
     1801 gcggtcgtga cacaatcatc acgcgttctg cacgtacaca cagacgatct tctggcggaa
     1861 ctgcggaggg cgaggaagga actggcggaa gcgaggacgg agatggagaa gtcatcgaac
     1921 tcccgcgttg tcccatctgc cacacctttg tcctccgagc cggagttgac ggctgcgtgg
     1981 tatgcagaga atcagcgcag gatgacggag cagatgttcc gggggttggg ggtggactta
     2041 aatgcattgc atgcctcgct tacgacgcca aagtcgtgat cgagtacgca ggcaagtcct
     2101 ttcactactg caacacctgc tatcacacct gcaacaagct gatccatgca gaggacaaga
     2161 cggtcactct gtgccacaat gacaccaatc acatgctcaa ggatggaact cgggtgtgtc
     2221 acatccacgt tcgtaaccag agtggttcgg tgtccgacac caccatgcgg aagcgtggag
     2281 cttcaatcac ggaggtcaag taggaatgtt ctttttgttc cacaagacca cccgaccgac
     2341 cggacgcttg ctggcaaagg ctcttggcat tcggcacttc ggttccgttg tcaaagctgg
     2401 catccagcct gacttcctga ttcggtgggg caatgcaatg cctgtggctg gtcggtacac
     2461 ggaggtcaat tctccggccg caatcagcgc agcaagcaac aaactgaggg cgttccgtaa
     2521 gttccatgag gcagggatga gtatccctaa gtttacggac tcacggtacg aagcggtgga
     2581 tttgcagcgc caaggctaca caatcttcgg ccgaactagc tccggcacga aggggcgcgg
     2641 cattgtgatc taccgtccag ggcaggaaat cgggtaccac gaactgtaca cgggctttat
     2701 cccgaacatc agggagtatc ggctgcatgt agttggagac tccgtgattc gtacacaacg
     2761 gaagtatccc gagcgtgctg gtgcgacagc cgacgcgccc atcaagaacg tggagcatgg
     2821 cttcgtgttc aaagcgccac agcgcaagct gaacaaggat cgtgaggatt tggccgtgag
     2881 tgcggtcaag gctcttggtc tggacttcgg tgcagttgac atggtgatcg acgagaacaa
     2941 cgtcgcctat gtcctggaaa tcaacaccgc tccggcgtgc tcaccaatga caggcacggc
     3001 ctatgtcaca gcattgaggg agttggtgtt ggcaaagtcc aacgggggtt atgaccttgc
     3061 aacaaacctg gatgcgctga agggcgcaac cgcatcaggc gaggtagacc cacgcaaccg
     3121 cgtcatggag gcgatcattg gccgttagcg accgcacctt cggtgtggaa atcgaatgca
     3181 cttacggcgc acgtccttca aagaaatcag atgtgtacga acgcatccgt tatgctgaga
     3241 gg
//
LOCUS       DATMTI010000203         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_50620, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000203 DATMTI010000000
VERSION     DATMTI010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50620"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            725..798
                     /locus_tag="VN039_03430"
     tRNA            725..798
                     /locus_tag="VN039_03430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:757..759,aa:Gly,seq:ccc)
     gene            815..>1695
                     /locus_tag="VN039_03435"
     CDS             815..>1695
                     /locus_tag="VN039_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HWV45070.1"
                     /translation="MDANTLGDVASARWNQWHTIPWAEAFQHVRRLQARIAKAAKDEN
                     WRTVKRLQKLLVRSTTARAVAVRRVTENQGRKTPGVDGLTWSTPKEKWKAISALDSRG
                     YRPKPLRRIHIPKANGGKRPLGIPTMKDRAMQALHWFALDPVAETRGDLNSYGFRSGR
                     STADAIAQCHNALSREHSPQWVLEGDIKACFDNISHDWLVRNVPMDRRLLEKWLKAGF
                     VEGRKLFPTDAGTPQGGIISPCLANLALDGMERLLKDSLPRRDKVNFIRYADDFVVTA
                     VSKEALETKVKPLIVGFL"
BASE COUNT          373 a          468 c          543 g          311 t
ORIGIN      
        1 ggccacgaag gtgcaatgcc tcgtggttca gtgggataga cggttgtatc cagcaattgg
       61 atgcgacgtc acgcggcgaa ggccgtgcaa gtaattgtcg gatgaggtgc atgcgcttca
      121 ctccgacctg atgttcagcc atcagtagcc taggatcagt gcggggcggg taactgcccg
      181 gtgccgagcg atctgacaat gtagccaccc tcacgggtac gtcagccccg tgaggggcgg
      241 gcgtgacctc ggaagatgta tccgagtagg cgctaggctg tgcaacaggg ttagcgcaag
      301 cgaaccatca tctaaacgtc gtcaagctag ctgggccaaa gacatctgac aggccctacc
      361 caaaacggga cgaggtcgga cacttccctg cttggtggaa gtgcgctgat accagaacct
      421 ccggcgaaaa gatggaaccc tcgttggtac tgttgcttgt acggaacgtg gcaatcccgt
      481 taggctgtcg gcgcttcttg gccgactagg cgttccgtga ggaacgttga acgcgtagcg
      541 ggcttaggat cgcggaaaaa gcgaaggccc ggctgtaatg gccgggatag gggatgaaac
      601 gatcgccccg acccgaaagg gtgcagactt ccgcgtggtg gcctgtcgct agacaggtct
      661 gacaaccgac tcccgatggg gtgacgatcc ccatcagggg gcgtccatcc ccaaagggtc
      721 atgggcgggt gtagctcaat ggtagagctg ttgcttccca agcaactgac gagggttcga
      781 ttcccttcac ccgctccaac tttggaggac tgatatggac gccaatactc ttggcgatgt
      841 cgcctccgcg cgctggaacc agtggcacac gatcccgtgg gctgaagcgt tccaacacgt
      901 caggaggttg caggcacgca tagcgaaggc agcaaaggac gaaaactggc gcaccgtgaa
      961 acggctgcaa aagttgctag tccgctcgac caccgcacga gcagtcgcgg tgcgtcgagt
     1021 cacggagaac caaggccgga agacccccgg tgtggacggc ctcacttggt ccacacccaa
     1081 ggagaagtgg aaggcgatat cagcgctgga ttcgcgcgga taccgtccca agccgcttcg
     1141 caggatccac atcccgaagg ccaatggcgg gaagaggccc ttaggcatac ccacgatgaa
     1201 ggaccgagcc atgcaggcgc tgcattggtt tgcactcgat cctgtcgcgg aaacccgagg
     1261 tgatctcaac tcgtacggct ttcgctccgg acgttcaacg gcagatgcca tcgcccagtg
     1321 ccataacgca ctgagccgag aacattcgcc gcaatgggtg ctggagggcg atatcaaggc
     1381 gtgcttcgac aacatcagcc acgactggct ggtgcgcaac gtgccgatgg atcggcgctt
     1441 gctggagaag tggctcaaag ccgggtttgt ggagggtcgg aaactgttcc cgacggatgc
     1501 aggtacgcca caaggcggga tcatctcccc gtgtttggcg aatctggcat tggacggcat
     1561 ggagcggttg ttgaaagaca gccttccacg ccgggacaag gtgaacttca tccggtatgc
     1621 ggacgacttc gtggtgactg cggtctcgaa agaggctctg gaaaccaagg tcaagccgct
     1681 gatcgtgggc ttcct
//
LOCUS       DATMTI010000204         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_517853, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000204 DATMTI010000000
VERSION     DATMTI010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517853"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2176)
                     /locus_tag="VN039_03440"
     CDS             complement(<1..2176)
                     /locus_tag="VN039_03440"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWV45071.1"
                     /translation="MPFDQALRFASIWLASTSFIGLTLGAGLPEWLAALTGAALIVAL
                     LANRGGKAVERLATVTPISTSGWNLLVIVGFLGFWVDMLWVSGELLPAGVHFLMVLMI
                     IKLFNLKLRRDYLHLYAISLVAILASGSLTTDLWYLPIFLLYLLAGVWTLLLFQLTKH
                     SEETRMLLMSVTRRPQFPASVGHVTSQLFWLANGLALATFGLTLVIFFTIPRVSAGFY
                     QKGFGENIRTSGFSETVNLGAIGPIKRDPSVVMRVELPVSSPHEASPFYLRGVAFDQY
                     DGKSWTNRLNYRRAVSEDTTGTFVLRGSRSHASPLLGETVRQNILLEPLDTSVLFAAP
                     FIERVSGKFPNIQSDLTGSVYLPFPSSSRIEYSVLSRSNPVLPADLGSEPSLYPESFT
                     RHFLQIPGQSERIAALAREITHTQRTAYDKANAIQSFLTHNFRYSLDAPLTDKGQPLE
                     EFLFTRKTGYCEHYASAMVIMLRTIGIPARLVTGFLATEWNEYGNYYLVRQQDAHAWV
                     EVHLPHSGWITMDPTPPLIENVGSEFPTWHALERMMDTFRLQWSRFFVQYSAADQLAI
                     VRELKAGGTSARNKAFDSIGALFSPFVTMFSGITEYASKGTILSLVELLGISLIGLAV
                     LWLGIKRFWVREMVSKKAARDEQVIAQLYGRMVQHLAGEGISKPTTTGPLEFVRIAQA
                     KWSGANSAVASITELYCRTRFGHISLTKEELSLAEDHLRHLMA"
     gene            complement(2166..>2751)
                     /locus_tag="VN039_03445"
     CDS             complement(2166..>2751)
                     /locus_tag="VN039_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWV45072.1"
                     /translation="IKPIDERLVRGLLTMGPEQSVDRRGHGNDLYNLRLYQAGDDSRK
                     IHWPTTARTAQLTVRETEAEDQRRAIVYLPTVAPVSHDALFEQAVSLTASIIQHLAHR
                     GYSLQLLVGSSHSSFGQGDLHLWEFLRMLALCERRPPSAETIMPEELPRGSWEVEGGM
                     MIVVQSWMGPGDIEPQHPTILINGNIITGASHAV"
BASE COUNT          657 a          756 c          767 g          571 t
ORIGIN      
        1 gtgccatcaa atggcgaaga tggtcttcgg cgagggacag ttcttccttt gtgagggaga
       61 tatggccgaa tcgtgttcgg caatagagtt ccgtaatgga cgcgaccgcg gaattcgcac
      121 cactccattt cgcttgagcg atgcggacga attcgagggg acctgtggtg gtgggtttcg
      181 aaatgccttc tccagcgaga tgttggacca ttcgtccata gagttgggcg atcacctgct
      241 cgtcgcgcgc cgccttcttg ctaaccatct ccctgaccca gaaccgcttg atccctagcc
      301 agagaacggc gaggccgatc agcgaaatgc caagcaactc caccaaagac agtatcgttc
      361 ctttagatgc gtattccgta atcccactaa acatggtcac aaaagggctg aagagcgcgc
      421 ctatggaatc gaatgccttg ttgcgggccg acgtacctcc cgctttcaat tcccgcacaa
      481 tggcaagctg atccgccgca ctgtactgca caaaaaatcg gctccactgg agcctgaagg
      541 tatccatcat tcgttccaac gcatgccatg tcggaaactc gctgccgaca ttttcgatca
      601 agggcggagt gggatccatc gtgatccacc ccgaatgcgg cagatgcacc tccacccacg
      661 catgggcgtc ttgctgtctg accaaataat agttgccgta ttcattccac tcggtggcga
      721 gaaaccccgt cacgaggcgc gccgggattc caattgtccg aagcataatc accatggctg
      781 aagcgtagtg ttcgcaatac ccggttttgc gggtaaagag aaactcttca agcggctgac
      841 ccttgtctgt cagaggcgca tcaagactat agcgaaagtt gtgcgtcagg aacgattgga
      901 tggcgttggc tttatcatag gccgtgcgct gtgtgtgagt gatttccctg gctagggccg
      961 caatccgctc ggattgacca gggatctgca agaaatgtcg ggtaaatgat tcaggataga
     1021 ggctaggttc cgaaccgaga tccgccggca gtaccggatt ggatcgggaa aggacggaat
     1081 attcgatccg cgacgaactg gggaacggta aatagaccga accggtgaga tctgactgga
     1141 tgttcgggaa ctttccactg accctttcga taaagggagc agcaaagaga accgaggtgt
     1201 cgagcggttc caaaaggatg ttctgccgga cggtttcgcc gaggagaggt gaagcgtgag
     1261 atcgactacc gcgcaggaca aacgtcccgg tcgtgtcttc gcttaccgcc cgccggtagt
     1321 tcagccgatt cgtccaggac ttgccgtcgt attggtcgaa tgcaacacct cgtaggtaaa
     1381 atggactcgc ttcgtgcggg gagctaacgg gcaactcaac ccgcatcacg acgctgggat
     1441 cccgcttgat aggcccgatc gctcctaagt tcaccgtttc ggagaatccg gacgtacgaa
     1501 tattctctcc aaagcctttt tgataaaacc ctgcactgac ccgtggaatc gtaaagaaga
     1561 tcaccaacgt caggccaaag gtggcgagtg caagcccgtt tgccagccaa aagagttgtg
     1621 atgtcacatg gccgaccgat gcaggaaatt gtggtcgcct cgtgaccgac atcaagagca
     1681 tccgggtttc ctccgaatgt ttggtcagct gaaacaggag cagcgtccac acgccggcca
     1741 aaagatagag caagaaaata gggaggtacc acagatccgt cgtgagggac ccggaagcca
     1801 ggatcgctac gaggctgatg gcgtagagat gcagataatc acgacgcagc ttgagattga
     1861 acagcttgat gatcatgagg accatgagga agtgaactcc tgccggaaga agttcacccg
     1921 agacccacaa catatccacc caaaatccaa gaaagccgac gatcacgaga agattccatc
     1981 cgctggttga aatcggcgta accgtggcaa gtcgctccac agccttgcct cctctgttcg
     2041 ctaagagagc aacgatcagc gccgctcccg tcagcgcagc cagccattcc ggcaaacccg
     2101 ctccaagcgt cagaccgatg aaggaggtgg acgccagcca aatcgatgcg aaccgaaggg
     2161 cctgatcaaa cggcatgcga tgctccagtg atgatgtttc cgttaatcag aatggtggga
     2221 tgttggggct cgatatctcc cggtcccatc catgactgca cgacaatcat catccctcct
     2281 tcgacctccc aggagcctct gggcagttcc tccggcatga tggtctctgc agagggcggt
     2341 cgccgttcgc agagagcgag cattctaaga aattcccaga ggtgcaggtc gccttgaccg
     2401 aatgacgagt gagacgaccc caccaataat tgcaggctat acccgcggtg tgccaaatgc
     2461 tgaatgatgg aggccgtgag ggagaccgcc tgttcaaaca gggcatcgtg gctcactgga
     2521 gcgactgtcg gtaaatacac gatggctcgg cgttgatctt cggcctcggt ttcccggacc
     2581 gtcaattggg ccgttcgcgc cgtcgtcggc cagtgaatct ttcgcgagtc atcccccgct
     2641 tgatacaggc gcagattgta caaatcattg ccatgccctc gtcgatcgac actctgttcc
     2701 ggtcccatgg tgagaagacc ccgtactaga cgctcgtcga tcggcttgat t
//
LOCUS       DATMTI010000205         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_305916, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000205 DATMTI010000000
VERSION     DATMTI010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305916"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VN039_03450"
     CDS             complement(<1..1017)
                     /locus_tag="VN039_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV45073.1"
                     /translation="MTQTAAESDYPTILVVDDNPSALYVKSRLLSRQDYRIIEADNGA
                     DALTVATAERPALILLDVNLSDISGFDVCQQLKTQPETKFIKILQTSAARINAPDRVK
                     SLEVGADAYLVEPAEEEELIGTVRALLKLAQHERDNQRLIERLTESEMRYRSLIERMP
                     AAMYTIDRHGRITFYNDQAAELWGRRPNLGDSESQFRGSHKLLWPDGTPLPHDKTPMA
                     DAVQDGTALHGQDVILERSDGTRRHVIVHIDPLRNAEGHIVGAVNLFTDITVRKQAED
                     ALRDAERRYRTIFEQAGVGVAQIDSRTGRFERVNRKYCEIVERTEAEMLATTFMSITH
                     PDDLA"
     gene            complement(1014..>2845)
                     /locus_tag="VN039_03455"
     CDS             complement(1014..>2845)
                     /locus_tag="VN039_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWV45074.1"
                     /translation="PFGCHEAISVGERETPRPLPSEWYGLRRMNQQAWNSEDEPHQTG
                     VPYDWYYRISRWFVDTVALIAVLVLAGWTFHINRLTSLSPDFASMKVTTAIGLLLLAG
                     ALLLLNPHIVRPDPSGTRVRNGIVFVLAAMAGLLGLGTLLGYAMHVEGERVPPALTAP
                     ATALCLVFLGAAVPASMKQNARQYVSQSLAAVVMFICSLAVLGYLFHKQSLYAIPSYK
                     SMALHTAVSFVLLSLALLCLRPDRGLMKTATAGTAGGSMLQRMVPLMVGMVLIIGWVH
                     LMGETAGLYNSRFSLLMAVGLSVVGFGVILWTVAGSLNQIDAAKGLSERRLQHAIKQL
                     RASDEQKTRLLYTVSHEYRTPLNGIIGISRFLLNRTDGPLTPEQDKQIKMMQTGAQQL
                     LVLIDAMLDLDKIKTGKLQPASRRCTVPSLLQSLRAAAELFPSRPGVSLVITEPDSLP
                     ELMTDDTLLSRILHNFVHNAVKYTEHGTIIVSAESVSNGRAVRFVVRDSGIGIAPEDQ
                     ARIFDEFVQIRHPLQQGIRGQGLGLALCKRIADVLDASIMVESQPGKGSTFSVTVPCE
                     QKTSVAMVEDEWQEQAQVDMAESGTFKSAECRSGSPMSLVRVI"
BASE COUNT          588 a          833 c          810 g          614 t
ORIGIN      
        1 cgcgagatcg tcaggatggg taatactcat gaaggtggtc gccagcatct cggcttctgt
       61 ccgttctaca atctcacagt atttccgatt gacccgctcg aatcgtcctg tgcgcgaatc
      121 gatttgtgcc actcccacgc cggcctgttc aaaaatggtg cggtatcggc gctctgcatc
      181 gcgtagcgcg tcttcagcct gcttgcgtac ggtaatgtcc gtgaacaagt tcaccgcgcc
      241 gacgatgtgt ccttcggcat ttcgtagagg atcgatgtgg acgatcacgt ggcgccttgt
      301 gccatcggag cgttcgagga tgacgtcctg accatgcagc gccgtgccat cttgcaccgc
      361 atcggccatg ggcgtcttgt catgcggcag cggtgtccca tccggccaca gcagcttatg
      421 cgacccccga aactgacttt cgctgtcgcc caggttcggc ctgcgacccc acaattcggc
      481 tgcttgatcg ttataaaacg tgatacgacc gtgtcgatca atcgtataca tggccgccgg
      541 catgcgctcg ataagagacc gatagcgcat ttcgctctcg gtgaggcgtt cgatgagccg
      601 ctgattatcc cgttcgtgct gggccagctt gagcagtgcc cgcacagtgc cgatcaactc
      661 ctcttcctcc gccggttcga ccaaataggc atccgccccg acctccaggc ttttcacccg
      721 atccggcgcg ttgatgcggg cggcggaggt ctgcagtatt ttgatgaact tcgtctcagg
      781 ctgagttttg agctgctgac agacatcgaa accgctgata tccgataaat tgacgtcgag
      841 caggatgaga gcaggccgtt ctgcggtggc aacagtgagt gcgtcggcgc cgttgtccgc
      901 ttcaatgatt ctataatcct gccggctgag caatcgactc ttcacgtaca aggctgacgg
      961 gttatcgtcg acaacgagaa tggtcgggta atcggattcg gcggcggtct gagtcatatc
     1021 accctcacga gactcatcgg agacccggac cggcattcgg cactcttgaa cgttccgctc
     1081 tcggccatat ccacttgcgc ctgctcttgc cattcatctt cgaccattgc caccgatgtt
     1141 ttctgctcac agggcaccgt gaccgaaaag gtcgagcctt tcccaggctg actctcaacc
     1201 atgatgctgg catcgagcac atcggcaatc cgtttgcaca gtgctagacc gaggccctgt
     1261 cctctgatcc cctgttgcaa gggatgacgg atttggacga attcgtcgaa gatgcgagcc
     1321 tgatcttcgg gagcgatgcc gatcccgctg tcgcgcacga caaaacgaac agcccggccg
     1381 tttgagaccg actcggccga gacgatgatc gtgccatgct cggtatattt caccgcattg
     1441 tggacgaaat tgtggagaat gcggctcaag agagtatcgt cggtcatgag ttcgggcagt
     1501 gagtcgggct ccgttataac gagactcacg ccgggtcgtg aaggaaacag ttctgcggcg
     1561 gcgcgtaggc tctgcaacaa tgagggcacc gtacatcggc gggatgccgg ttgcagtttg
     1621 ccggttttga tcttatcgag atccagcatc gcatcgatga ggaccaacag ttgctgggcc
     1681 cctgtctgca tcatcttgat ttgtttgtcc tgctccggcg tcagcggacc gtcggtacga
     1741 ttcagcaaaa accggctgat gccgatgatc ccgttcagtg gggtgcgata ttcatggctc
     1801 accgtgtaga ggaggcgggt cttttgttcg tcggaggcgc gaagttgctt gatggcgtgc
     1861 tgcagacgtc gctcgctcag accctttgcc gcatcgattt ggttcaagga ccctgctacg
     1921 gtccacagaa tgacgccgaa tcccaccaca ctcagcccga cagccatgag aaggctgaag
     1981 cggctattgt acaatccggc cgtttctccc atcaggtgga cccatccgat gatgaggacc
     2041 atccccacca tcagcgggac catgcgttgc agcatcgagc ctcccgctgt gccggcagtc
     2101 gccgttttca tcagccctcg atcgggccgg agacagagca gggcgaggct cagcaacaca
     2161 aacgagacgg ccgtgtgcag cgccatcgac ttgtacgacg gtatggcata gagcgattgc
     2221 ttgtggaaga gataccccag cacggcaagc gaacagataa acatcacaac cgcggccaga
     2281 ctttgagaaa catattggcg ggcgttctgc ttcatcgaag cggggacggc tgctcctagg
     2341 aacacaaggc atagagccgt tgccggcgcc gtcaatgccg gaggaactct ctccccttcc
     2401 acatgcatgg catatcccaa caaggtgcca agccccagca gaccggccat cgctgccaat
     2461 acaaacacga tgccgttccg aacgcgcgtg ccgcttgggt ccggacggac gatatgcggg
     2521 ttgagcaaca gaagggcgcc ggctaagagc agcaagccga ttgccgtggt gactttcatc
     2581 gaggcgaagt cgggagacaa gctggtgagt cggttgatat gaaatgtcca gcctgcgaga
     2641 accagcacag cgatgagtgc caccgtatcg acaaaccacc tcgagatccg atagtaccaa
     2701 tcatagggca ctccggtctg gtgcggctca tcctcggagt tccaagcctg ctgattcatg
     2761 cgccgtagac cataccactc ggaagggagt ggcctcggcg tttctcgctc ccctactgat
     2821 atcgcctcgt gacaaccaaa tggtt
//
LOCUS       DATMTI010000206         5834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_305959, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000206 DATMTI010000000
VERSION     DATMTI010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305959"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1047)
                     /locus_tag="VN039_03460"
     CDS             complement(175..1047)
                     /locus_tag="VN039_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWV45075.1"
                     /translation="MITCVLLIIMGSPSIVEAGSTPSPELTRHLTEIARLARPSPMIT
                     IPAGTFLLGSNRIDNDPYGNSTQFDDTELPQHRVWLNTYEMDRDEVSLGEYLAFLQKQ
                     KLHPSDELQKLLWHVITVHSVSDQTLSRWPALYVTWAEANNLCMSKQARLPTEAEWEK
                     AARGSEGIAYPWGEATPSSTLAMFGQHHVHEIPILAAVDSYEEGKSPYGLHHMAGNIA
                     EWVQDWFGFDYYAYMPERNPPGPTTGRYKSVRGGSWKSNRVMLRTATRGGSSPDQRAA
                     TIGFRCARSLKNAG"
     gene            1260..2294
                     /locus_tag="VN039_03465"
     CDS             1260..2294
                     /locus_tag="VN039_03465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWV45076.1"
                     /translation="MSTHHLFAAHYVRLPTLGVWIATLMLTLPLNAETTPLGQDAFLR
                     ARQVTVGILADTQDQRMPEKPGKVVVRGTGFHLRDGYVITARHTADKHELATGTVIQK
                     QIRILTNDLHELPADLVGDSAFMDIVVYRVAEQHRAKLQAGTSFAADEVMPGMEVFTV
                     GYPLGWGPTMAFGRLGNTNTFLQTVDTRLIQADLSTCSGNSGGGLFNARGEIVGIVHA
                     VIQTDKEETQAHCSRMTFVIPGTLSQRIIDAALSGRPLAFSRLGVHMTSVKDGTKLRV
                     AVKEVAEPAKAAGIQKHDILLAIEGTEILDGAQLKNFLIERTSPGQEVSVKVRRVDVD
                     LTFHVVLGGG"
     gene            complement(2381..3388)
                     /locus_tag="VN039_03470"
     CDS             complement(2381..3388)
                     /locus_tag="VN039_03470"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWV45077.1"
                     /translation="MDGTTNHLTGATLSAADPESIRSAGRIIKTGGIVAFPTETVYGL
                     GCDAMNADAAAKVFEAKQRPQFDPLIVHIADPKQLDVVIGSLPVLGQRLMDAFWPGPL
                     TLVLPKQRAIPDLVTAGLSTVAVRMPNHPVAQAFIREAGTPIAAPSANPFGYVSPTTA
                     QHVVDGLGSKADLILDGGACPVGVESTIVSLAGLQPEILRPGSITIEQLSAVIGSIRR
                     SSPVNQTPIAPGQLARHYATRTPLTILASVGARPVVKDGEHAGLLIHSQASDADDRFA
                     AVEVLSATGDLREAARHLFAALRRLDTLGLDRIYAEPCKEEGLGLAIMDRLRRCAAPR
                     Q"
     gene            complement(3414..3941)
                     /locus_tag="VN039_03475"
     CDS             complement(3414..3941)
                     /locus_tag="VN039_03475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWV45078.1"
                     /translation="MTMALEFRKKDPRVTISTRFGEIKIRFYSDAAPRHVENLINLVK
                     MGFYDGTTFHRVVPGFIIQGGDPLSKTPERTLHGTGGPGYFLSPEPNDRPHKRGAVSM
                     AKMPRESNSTRDFNDNGSQFFICVGENGGLDRRYTVFGEVFRGIEVVDKIVGVPRDER
                     DNPIDPIRIMMTVKE"
     gene            complement(3997..5292)
                     /locus_tag="VN039_03480"
     CDS             complement(3997..5292)
                     /locus_tag="VN039_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV45079.1"
                     /translation="MTNRAASPSPLRWLILGLLFAVSIVTYIDRVNISVTARQMMPAL
                     GLTEQEMGFVFSAFVIGYALFQIPGGWLGDRWGIRVVLMIALIWWSCFTAWTAVAATS
                     FLAVPLGIVGALALVRFLLGVGEAAALPTFNRAVTDWLPDHERGLGIGIAISGIGIGA
                     AITPPVTAWIMVNYGWQSAFYLSSGIGFALAVICWFLVADRPSLHPWCTHGDATPSTI
                     PAAPARPSIPWAILRRTPSVWWLMLSYGCLGYVAYVYMSWFYLYLVNERGFNVLRGGL
                     FAAAPFLAILVSCPLGGWVTDRLAIRRGVTRGRQLVGMIGMGLAAGSIALGAAAESPY
                     LAIASLSLGAGWLYFTVGAYWSSTSDLSPTHAGSLSGLMNMGANLGGAISPTLTPWLA
                     QQWGWGASLGLAAFISFLGGIMWLRIRPGDGLTRGGAAC"
     gene            complement(5427..>5834)
                     /locus_tag="VN039_03485"
     CDS             complement(5427..>5834)
                     /locus_tag="VN039_03485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWV45080.1"
                     /translation="AVAAESEKKAGSAFTAKAASEKGATKTESGIVITTIKPGTGATP
                     KATDTVKVHYHGTLTDGTVFDSSVKRGEPATFPLNKVIKCWTEGVQQIKVGGKSRLVC
                     PSNLAYGDGGSPPVIKPGATLVFEVELLDIVTN"
BASE COUNT         1331 a         1613 c         1665 g         1225 t
ORIGIN      
        1 aggtccggac acaggtacaa agaaagtgat caggggcgct ggctggcaaa atgagacgcc
       61 gacggtccgc atctttactc gtgtggagag cgatccgatg gttcgaaacg agtccacagg
      121 atttcgttgc gcatccgatg caccgaaaca atcaacaaag aatccggcaa ctccctaacc
      181 cgcattcttc aatgatcgcg cgcaccggaa gccgatcgtc gcggcacgtt ggtccggaga
      241 ggaaccgcct cgggtcgccg ttcgaagcat gacgcggttg cttttccaag agccaccccg
      301 aacactcttg tagcgcccag tggtcgggcc aggcggattg cgctccggca tatacgcata
      361 gtagtcaaac ccgaaccaat cctgcaccca ttcggcaatg ttgcccgcca tatggtgcag
      421 accataggga cttttacctt cctcatagga atcgactgct gcgagaatgg ggatttcgtg
      481 gacatggtgc tgcccaaaca tcgcgagcgt ggagctggga gtcgcttcac cccatgggta
      541 ggcaatgcct tcagaccctc gcgccgcttt ctcccattcg gcctcggtcg gtagtctggc
      601 ctgctttgac atgcatagat tgttcgcctc tgcccatgtg acatagagcg cgggccaacg
      661 ggatagggtc tggtcggaga cggaatggac ggtgatgacg tgccagagga gtttctgcaa
      721 ctcgtccgag ggatgtagct tctgtttctg cagaaaagcc aggtattctc caaggcttac
      781 ttcatctcga tccatctcat aggtattcag ccagacgcga tgttgaggca gttccgtatc
      841 gtcgaattgg gtcgagtttc cataggggtc gttatcgatt ctgttgcttc cgagcaaaaa
      901 cgtgccggcc gggatggtga tcatcggcga cggtctggcc agcctcgcga tctctgttaa
      961 atgacgggtc agttccggcg agggcgttga gccggcctct acgatggagg ggcttcccat
     1021 gatgatcagg agaacacatg tgatcacgga ccatgcacaa caaggcggca tgacgtcttg
     1081 cccgagcaat tcttggcgag tccaaatcgc gttgcactat aaactggacg tctgtagctt
     1141 aacaatggtt cgggctgacg gtcagaacgt tctgagtcag tcgacacctt tcagaaccaa
     1201 cgcgatccga ttatagtatg cagcgccgac ttgtacatga gtttggaact gagtattgaa
     1261 tgagcacgca tcacctattc gcagctcact acgtgcgcct tcccacgctc ggcgtgtgga
     1321 tcgccacgct gatgctcacc ctcccgctca atgcggaaac gacgccgctg ggccaggatg
     1381 cgtttcttcg agcgaggcag gtgaccgtgg gaatcctggc tgacacacag gaccagcgaa
     1441 tgccggaaaa gccagggaag gttgttgtcc gaggaaccgg atttcatcta cgagacggct
     1501 atgtgattac cgccaggcat acggcagata aacacgagct tgcgacaggt accgttattc
     1561 agaaacagat tcgcatactc acgaatgatc ttcatgaact tcctgccgat ctcgtgggtg
     1621 acagtgcgtt catggatatt gtcgtgtacc gagtcgccga acaacatcgc gccaaattgc
     1681 aggcggggac atcatttgcc gcggacgagg tgatgcccgg gatggaggtc ttcaccgtcg
     1741 gctatccgct gggctggggt ccgaccatgg cctttgggag gcttggaaat accaatacat
     1801 tcctgcaaac cgtcgataca cgccttatcc aggccgatct ttcgacctgc agcggaaact
     1861 caggaggcgg gctgttcaac gcgcgaggag aaattgtcgg tatcgtgcat gccgtgatcc
     1921 aaaccgataa agaggagact caggctcatt gcagtcgcat gacctttgtc attccgggaa
     1981 ctctctcgca gcgcatcatc gacgcagctc tttcggggag gcctcttgcc ttctcaagac
     2041 ttggggtcca catgacttca gtaaaagacg ggacgaagtt gcgcgtagct gtgaaggaag
     2101 tcgcggaacc ggcaaaagcg gcaggcattc agaaacacga tatcttactc gccatcgaag
     2161 gtacggagat tcttgacgga gcccaactga agaatttcct catcgagcgt acgagcccgg
     2221 gtcaagaggt gtcggtcaaa gtgcgtcgag ttgatgttga tctgacattc catgtcgtgt
     2281 tgggtggggg gtgagggcaa taagctggta gcgggtgaat ggattttcac gtgttatcgg
     2341 atcctcaaag catcaagaga ttgcaaggac atgagcagcg tcattgccgt ggcgctgcgc
     2401 agcgtcgcag tcggtccata attgcaagcc cgagcccctc ttctttgcag ggctccgcat
     2461 agatcctgtc cagaccgagg gtgtccagcc tccgcaacgc tgcaaacagg tggcgtgcag
     2521 cttccctcag atcaccagta gcagatagaa cttcaacggc tgcaaagcga tcgtcggcat
     2581 cgctggcttg cgagtggatc agtaagcccg cgtgttcgcc atctttcacg acaggtcttg
     2641 ctccaactga agctaagatc gtcagtggcg tccgcgttgc gtagtggcgt gccaattgac
     2701 cgggggcgat cggcgtttga ttcacaggcg acgatcgtcg gatagagccg atgacggcgc
     2761 tgagttgttc aatggtgatg ctgcctggac gcaatatttc cggctgtagc cctgccagtg
     2821 aaacaatcgt cgattcgacc ccaaccgggc atgctcctcc gtcgagtatg agatctgctt
     2881 tgcttccaag cccatcaaca acatgttgcg cagtcgtcgg gctcacatat ccaaagggat
     2941 tggcgctggg cgctgcaatc ggagttcctg cttctctaat aaacgcttgt gccaccggat
     3001 ggttgggcat tctgaccgct accgttgaaa gcccggcagt gacaagatcc gggattgccc
     3061 gctgtttggg taacaccaaa gtcagtggac cgggccaaaa ggcgtccatg agccgctgac
     3121 ccagaacggg cagggatccg atcaccacat ccaactgctt tggatcagcg atgtggacga
     3181 tgagtggatc gaactgagga cgttgcttgg cttcgaagac cttcgctgct gcgtcagcat
     3241 tcatcgcatc gcaaccgagt ccatagaccg tttcggttgg gaacgcgacg attccacctg
     3301 ttttgatgat ccttcctgct gaccggatgg attcgggatc tgctgccgag agggtggcac
     3361 ccgtcaaatg attggtcgtt ccatccatag gaatgccgca cgatgccggt cacctactcc
     3421 ttcacggtca tcatgatcct gatcggatcg atggggttgt ctcgctcgtc gcgcgggaca
     3481 ccgacaatct tgtcgacaac ctcgatccca cgaaagactt ctccaaacac ggtatagcgt
     3541 cgatctaaac caccgttctc tcccacgcag ataaaaaatt gtgagccgtt gtcattgaag
     3601 tcgcgcgtac tattgctctc acggggcatt ttggccatgg ataccgcgcc gcgcttgtgt
     3661 ggacggtcgt tcggctccgg agaaagaaaa tagcctggtc cgcccgtacc gtggagtgta
     3721 cgctcaggag ttttgctcaa tggatcgccg ccctgaatga tgaacccggg caccacccgg
     3781 tggaacgtgg tgccgtcata gaaccccatc ttgacgagat tgataaggtt ttccacatgg
     3841 cgaggtgccg cgtcggagta aaagcggatc ttgatctcac cgaacctcgt ggagatcgtg
     3901 acacggggat ctttctttcg aaactcaagt gccatcgtca tgaatgtagc aaggcagtct
     3961 tcatggtgac aaggtcttac caacagcact cgtatctcag cacgcagcac cgcctcttgt
     4021 cagcccatcc cctggccgta tgcgcaacca catgattcca cccaggaagg aaatgaaggc
     4081 agcgagaccg agagaagcac cccagcccca ctgttgggca agccatggcg tgagtgtcgg
     4141 agaaatcgcc ccgccgagat tggcacccat attcatgaga cctgagaggc tgccggcgtg
     4201 ggtcggtgac agatcgctcg ttgaggacca atacgcgccg actgtgaaat agagccagcc
     4261 ggcacccaat gataaactgg cgatggcgag atagggagat tctgcagcgg cacccagagc
     4321 gatggaacct gctgccagcc ccatacctat catgcccacg agctgacggc ctctcgtcac
     4381 tccgcgtcgg atcgccaatc gatcagtgac ccatccgcca agagggcacg atacaaggat
     4441 tgcaaggaac ggagcggcgg caaacaatcc accgcgtaag acattgaagc cacgctcatt
     4501 cacgaggtag agatagaacc aggacatgta gacataggcg acatagccca agcatccata
     4561 actcagcatc agccaccaaa cgctcggtgt tcttcgtaga atagcccacg ggatcgaagg
     4621 gcgtgctgga gctgctggaa tcgtggacgg ggttgcgtcg ccatgagtgc accatggatg
     4681 aagcgatgga cgatcggcga ccaagaacca gcagatgact gcgagagcga atcctatccc
     4741 gcttgagaga taaaaggcag attgccaacc gtagttgacc ataatccagg ccgttaccgg
     4801 cggggtaatt gctgcaccga tcccgatccc gctgatagcg atgccgatgc caagaccacg
     4861 ttcatgatcg ggtaaccagt cggtgacggc gcggttaaag gtcggtaatg ctgctgcctc
     4921 tccgacaccg agaagaaatc tgacgagggc cagtgcaccg acgattccga gcggcacggc
     4981 caggaaagaa gtggcggcaa cggccgtcca ggcggtaaaa caggaccacc agatcagtgc
     5041 aatcatgagg acgacgcgga tgccccagcg atcgccgagc cacccaccag gaatctggaa
     5101 caacgcatac ccgatgacaa atgcagagaa gacaaatccc atctcctgct cagtaagacc
     5161 cagcgccggc atcatctggc gcgccgtgac cgaaatgttg acgcgatcga tgtaggtgac
     5221 gatgctgacg gcaaagagaa gccccagaat cagccaacgt aggggtgaag gagatgcggc
     5281 gcgattcgtc aaagtcctct cgccaacaat agtcccccta tcagattcat ccagattcat
     5341 tggaagggtg gtcgttccgt ggttcggcag gcgtcgaggg attgaattgc ttgccgcctc
     5401 tttgcagagg cggcgaagcg aagtggtcag ttggtgacga tgtcgagtaa ttcaacttcg
     5461 aagaccaagg tggctcccgg cttaatgacc ggcggagacc cgccgtcgcc ataggccaga
     5521 ttggatgggc ataccagccg gctctttcct ccgactttaa tctgttgaac tccctctgtc
     5581 caacacttaa tgaccttgtt caacggaaat gtcgccggct cccctcgctt gacggaacta
     5641 tcgaataccg ttccatcggt caaggtgcca tggtaatgga ccttgaccgt gtccgttgcc
     5701 tttggcgtgg ctccagtgcc gggtttgatg gtggtgataa cgattcctga ctcggtcttc
     5761 gtcgcccctt tctccgaagc cgcttttgcc gtaaacgcag agccagcctt cttctcactc
     5821 tcagcggcga cggc
//
LOCUS       DATMTI010000207         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_135981, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000207 DATMTI010000000
VERSION     DATMTI010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135981"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VN039_03490"
     CDS             <1..196
                     /locus_tag="VN039_03490"
                     /inference="COORDINATES: protein motif:HMM:NF023520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HWV45081.1"
                     /translation="AWQASGQWRSASGEVIREPSYVLSLVHPDDVAPSKAVQEIITSY
                     KTRFRQEAVLRVKSHTCMSL"
     gene            complement(524..>1787)
                     /locus_tag="VN039_03495"
     CDS             complement(524..>1787)
                     /locus_tag="VN039_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV45082.1"
                     /translation="SNTIGDAELNRLQTESRRFGIITRDRSLLRLFQDVKKSAKSPLT
                     VLLLGEPGTGKELFARAIHRLSPRSEKNFIAVNMAAISPELFESELFGHVKGSFTGAT
                     SDRRGYFGLADHGTIFLDEIGDLRMDHQSKLLRVLQDKSFYRVGAAIPTTVDVRIVAA
                     TNRDLQKGVSEGWFREDLYFRLNGMVFHLPPLRERTEDIPLLADIFLNQLAEQIGKPV
                     PQLMNETLRALTDHDWKGNVRELRHCLEQAVTLNDGTLLTRDSLRLGEEHGQSRRRTG
                     QTVPELAGDTAVLNCLRRHGFDMQSTAKVLGWDRSTVTQRLKGLCFQALVEANGDRSK
                     AAMDIAGDPAHLRTAELKLRDYYDHLQSVIAPFTTVEEALVDCRRRFKNLPDRHFSSV
                     ETLVRRHFQQDRSIPFGQRTKRVGAEIQ"
BASE COUNT          346 a          498 c          508 g          435 t
ORIGIN      
        1 ggcttggcag gcgtccggcc agtggcgatc ggcatcaggt gaagtgattc gcgagccctc
       61 atatgtcctg agtcttgttc atccggatga tgtcgcaccg agcaaggctg ttcaggaaat
      121 tatcacgtcc tacaaaacgc gcttccgtca ggaagcggtg ctccgcgtca agtcccatac
      181 gtgcatgtct ctctaatccc tctcacacgg cttgaatagc caaggttccg catccgacac
      241 atccctttta caggttcttg cgcttccgaa ccgttgtgtt ggtcgtatct cgcttctgca
      301 agtctcctgg aagctttctc aatagtcctt gtcggcaata ccattcttga tagagttctc
      361 gtgttgaggt tgtaacagcg ggtattggtg ttatccagac atttaaaacc tgccattgcc
      421 tgggtcttcg tgcatctgca acgggtcgtg cttctgttgg tacttctact gtcttagggt
      481 tcggcttgcc ggcctgtgaa gcgttgccac tggagcggcc ccatcattgt atctcggctc
      541 cgacccgctt ggtccgctgt ccgaacggaa tggatcgatc ctgttggaaa tgtcgccgca
      601 caagcgtttc gaccgacgaa aagtggcggt caggaagatt tttgaaacgg cgcctgcaat
      661 cgacaagggc ttcttcgacg gtggtgaacg gcgcgatcac ggattgcagg tggtcataat
      721 agtcacggag tttcaattca gcggttcgta gatgggctgg atctccggct atgtccatcg
      781 cagccttgga tcgatcgccg ttggcttcca ccagcgcctg gaaacagagg cccttcaaac
      841 gctgcgtcac cgtactgcga tcccagccga gcactttggc cgtggactgc atgtcgaagc
      901 catgtcgtct cagacagttc agaaccgccg tatcaccggc tagctcggga accgtctgcc
      961 cggtacgtct tctcgattgc ccatgctctt caccaagtct gagtgagtcc ctggttaaga
     1021 gggttccatc gttcagcgtg acggcttgtt ccaaacagtg gcggagctcc cgtacatttc
     1081 ctttccagtc gtggtcggtc aatgcacgaa gcgtttcatt catcagctgc gggaccggct
     1141 tccctatttg ctcggcaagc tggttcagga agatgtcggc gagaagcgga atatcttcgg
     1201 tacgctcgcg cagcggcggg agatggaaga ccatcccgtt cagccggaag tacaagtcct
     1261 cgcgaaacca tccctccgac acacccttct gcaggtcgcg attggttgcg gccacgatcc
     1321 gaacgtctac agtcgtcggg atggcggcgc ccactcgata gaatgatttg tcttgaagca
     1381 cccgcagtaa cttgctctga tgatccattc gaaggtcgcc gatctcgtcg agaaagatcg
     1441 tgccgtggtc ggcgagaccg aaatagccgc gacggtcgct cgtcgcgccg gtgaagctgc
     1501 ccttcacatg gccgaagagc tcactttcaa acagctcggg agaaatggcc gccatgttga
     1561 cggcgatgaa gttcttttcc gatcgtgggc tgagtcggtg gatggcgcgg gcaaagagct
     1621 ctttccctgt gcctggttcc ccgagcagca ggacggtcaa cggagacttc gcgcttttct
     1681 tcacgtcctg aaacaaccga agcagggagc gatctcgggt gatgatgccg aatcggcggg
     1741 attcggtttg aagccggttg agttccgcat ctccgatcgt gttggat
//
LOCUS       DATMTI010000208         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_93542, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000208 DATMTI010000000
VERSION     DATMTI010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93542"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4063)
                     /locus_tag="VN039_03500"
     CDS             complement(<1..4063)
                     /locus_tag="VN039_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HWV45083.1"
                     /translation="MMSVQRIQRAYLAAILTSVSWLPFASAHADAVRPSEISSVDRNG
                     IDLVNGTVAYSTRDISIGNGESGLARVGSNFSTPYGDNFYGVVQYNDDGDEFLVTFNG
                     ETEAFDSTTFASKSASGNTLYCVSGICTYTLKDGTVVLFKTGYGGAQGVVGVFAITSV
                     TRPDGEIITFNYHRAQIANTMPKQYVSYLLSVSSSLGWMLKFHTLSDDPNVYPPSSVE
                     AINTSVDYCDPAAMACSGETVSWSRMSLSNTGPINALGQKTVFNGGMSAPISIVSPTG
                     VTKSITYYTSGTFSGMVHTVVVGSSTWTYAYSNSGIYPNYIQTTTVTNPDGGTHILTV
                     NTGTSQILSDTDPLGRKITYTYYTTTGSGALNGALYQIISPDATYSGATVTGGYSQYT
                     YDARGNILTETDVPKAGSGLSNLVTTYTYPSTCSNIKTCNKPTSVVDPNGVETDYTYD
                     ANSGNVATITTPSVNGVAAQERYTYTQVTPYIKNSSGGLVASTPVWRLSTVTSCMSGA
                     ASSCQGTSDEKKAVIGYGTNNALPVTVTTELGDNTRGATTTYTYDIYGDVTSVDGPFS
                     GSDDTVYYFYDALQRRTGEIDEDPDGSGSLPRHASRTTFNTDGQVSKVETGYTSGTDA
                     TALASMSVLTTTTTDFDPSSGLPVKLSQFAGTGGTALSVSQTSYDSLYRVSCNAIRMN
                     PSVYSSLPASACTLSTQGSDGADQITHYTYNSANTLLTTTTAYGTTAQTDAMTRTVNT
                     SSGTTATLKDGVGNLTTYTYDGFNRLTKVCMPSASNGAVSSTSDCSQISYTNMRPTSL
                     TLRDGQSESLGYDAAGRVNSRSGAGISESFTYNNFGQTKTHTNNGFTETLNYDGFGLL
                     TSDAQPAGTVQYAYDAYGRRSQLTYPDGFYVTYNYYSDGRGWSIRDNSGNYILQDEPA
                     SSGVLNDEKLGNASPYAVLRTFNYDGLYRLSGISSNLSTTSSTTAYDNVTSQALTAPG
                     QIKSRTLSNGAYEPPAPASGTTNYSLNGLNQVSAVSGAGFSYDSRGNLTSDGSASYGY
                     NADNLLTSVVAGSSTTSLSYDAQSRLYRVVKTGSAATRFLYDGSDLIAEYDDSGNVLR
                     RYVHGFGADQPVVAYYGSGTADSDRHYLVSDERGSIVAVTNNTGDIGAANTGVNTYNE
                     YGLPGSSNIGRFQYTGQTWLPEVGLYYYKARMYSPSLGRFMQPDPIGYDDGLNIYAYV
                     HGDPVNNTDPLGLETVVYVHGYAPVTDSAGDGGAGQDLQNERAAISAAVTERAKAAKI
                     DDIPVIKVVGRRRIINIHNNNIVFISTGSDNCQTVYVQCRVNREYYWRRYGRPANQNN
                     NYSPTNPSTCEGGKMMCDVITKERKGGTDRAGFG"
BASE COUNT          935 a         1043 c         1142 g         1008 t
ORIGIN      
        1 cgccaaatcc cgctcggtca gtcccgcctt tcctttcctt agtaattacg tcgcacatca
       61 tctttccacc ttcgcatgtg gatgggttcg tcggactata attgttattt tgattagctg
      121 gccgcccata acgacgccaa tagtactccc tatttacccg acactgcaca tacacggttt
      181 gacagttgtc agaaccagtt gatataaaaa caatattatt attgtgaata ttaataattc
      241 tcctgcggcc aaccaccttt attacaggta tgtcatcaat ctttgcggcc ttcgctcgtt
      301 cggtaaccgc agccgatatg gcagcccgct cattttgcag gtcttgcccg gcacccccat
      361 cgccagcact atcagttaca ggcgcgtacc catgaacgta gacaaccgtt tccaacccca
      421 aaggatccgt attgttcacg ggatcgccgt gcacataggc gtagatattc aagccgtcat
      481 catagccgat cggatcaggc tgcatgaagc ggccaaggct cggagaatac atccgcgcct
      541 tgtaatagta tagacccacc tcggggagcc aggtctgtcc cgtatattgg aagcggccga
      601 tgttgctgga tccgggcaga ccatattcgt tgtaagtgtt aacgcccgta ttggcggcgc
      661 cgatatcgcc tgtgttgttg gtgacggcga caatcgagcc gcgctcgtca ctgacgagat
      721 agtgccggtc tgaatcggcc gttcccgaac catagtaggc gacaaccggt tgatctgcgc
      781 cgaaaccatg cacgtaacga cgcagtacat tgccgctatc atcatattcg gcgatcagat
      841 cgctcccatc atacaagaag cgcgtcgcag ccgaaccggt cttgacgacg cgataaagac
      901 ggctctgcgc gtcatagctc aatgaggtgg tgctggagcc tgcgacgacc gaggtcagca
      961 ggttatcggc gttatagccg taggatgctg agccgtcaga agtcagatta ccgcggctat
     1021 catagctgaa accggcgccg ctcacggcgc taacctggtt gagtccatta agcgaatagt
     1081 tcgtcgttcc gcttgcaggc gctggtggct cataggcgcc attgctcagg gtacgggact
     1141 tgatctggcc gggcgctgta agggcctggg aggtcacgtt gtcataggca gtcgttgatg
     1201 aggtcgtgct tagattgcta cttatgcccg acaggcgata gagcccgtca tagttgaacg
     1261 tacgcaaaac agcgtaagga ctggcattcc cgagtttttc gtcatttagc acgcccgaac
     1321 tggctggctc atcttgcagg atataatttc cgctattgtc gcgaatggac cagcctcgac
     1381 catcggaata gtaattgtaa gtgacgtaga agccatcagg ataggttagt tgcgagcgac
     1441 gcccataggc gtcataggca tactggacag taccggcggg ttgcgcatcc gaagtgagca
     1501 ggccgaagcc gtcatagttt agggtttccg taaagccgtt gttggtatgt gtcttggtct
     1561 gcccaaagtt gttgtaggtg aaggactcgc tgatcccggc gccagagcgg gaattgacgc
     1621 gaccggcggc gtcatagcca agagattccg actgaccgtc ccgcagcgtc aagctcgtcg
     1681 ggcgcatgtt cgtgtagctg atctgggaac aatcgctcgt cgagctgacg gccccattcg
     1741 aggccgaagg catgcagacc ttggtcaggc ggttaaatcc atcataggta tatgtggtca
     1801 ggttccccac gccgtctttc aaggtggcgg tcgtgccgct actggtgttg accgtcctag
     1861 tcatggcgtc ggtctgggcg gtcgtgccat aggccgtcgt ggtggtcagc aaagtgttcg
     1921 cgctattgta cgtatagtgc gtgatctgat ccgctccgtc tgagccttgc gtactcaacg
     1981 tgcatgctga tgctggcaag gaggaataaa cggacgggtt catacgaatg gcgttacagc
     2041 tgacccggta gaggctgtcg tatgacgttt gggatacgct gagcgctgtt ccgcctgtgc
     2101 ccgcaaattg actcagcttt accggaaggc ccgaacttgg atcgaaatcc gtcgtcgttg
     2161 tggtgagaac ggacatggac gccagtgcag ttgcatccgt ccctgacgta tatccggtct
     2221 caactttgga aacctggcca tccgtattga acgtggtgcg gcttgcatgt cttgggagcg
     2281 atccactccc atcgggatct tcgtcgattt cgcctgtgcg gcgctgtagc gcgtcataga
     2341 aataatagac ggtgtcatcg ctgccagaaa acgggccgtc gactgaggtt acgtcgccgt
     2401 agatgtcata ggtataggtt gtcgtggcgc cgcgggtgtt atcaccgagt tctgtggtta
     2461 ccgtcacagg cagggcgttg ttcgtcccat atccaataac agccttcttc tcgtcggacg
     2521 tgccctgaca ggatgaggcg gcgcctgaca tgcacgatgt cacagtggat aagcgccaga
     2581 ctggcgttga agcgaccaaa cctcctgaac tgtttttgat gtaaggcgtc acctgggtgt
     2641 aggtatagcg ctcctgcgcg gcgaccccat taaccgaagg cgtcgtgatc gtggcgacat
     2701 tgccagaatt tgcgtcgtag gtgtaatcgg tctctacccc gttgggatcc acgacggagg
     2761 ttggcttgtt gcaggtcttg atattcgagc aggtcgatgg ataggtatag gtggtgacga
     2821 ggttcgacag gcccgaaccc gcctttggaa cgtcggtctc tgtcagaatg tttccgcgcg
     2881 cgtcgtaggt gtattggcta tagccgcctg tgactgttgc tccggaatat gtcgcatcgg
     2941 gcgagatgat ttgataaaga gcgccgttta gggcgcccga tccggttgtc gtgtaatagg
     3001 tgtaggttat tttcctgccg agagggtcag tatcggatag aatttgagac gtgcccgtat
     3061 tgacagttag gatgtgcgtg ccgccatcgg gattcgtgac ggtggtcgtc tggatgtaat
     3121 tcggatagat ccccgaatta ctataagcgt aggtccaagt cgaactcccg acgacgaccg
     3181 tgtgcaccat cccggaaaac gtaccgctag tgtaataggt aattgatttt gtgacacccg
     3241 ttggagaaac gatagagata ggtgcagaca ttcctccgtt aaatacagtc ttttgcccca
     3301 atgcattgat tgggccagta ttagataggc tcattctaga ccatgatact gtttcaccgc
     3361 tgcaagccat cgcagcagga tcgcaataat caaccgaagt gttgatcgcc tccaccgaag
     3421 atggtggata aacatttgga tcgtcgctta aggtatgaaa tttcaacatc cagcccagag
     3481 agctggaaac tgataaaaga tagctcacat attgcttagg catcgtattg gcaatttgcg
     3541 cacgatgata gttgaacgta ataatttcac catcaggtct ggtgacagaa gttatcgcga
     3601 atactccgac gacaccctgt gctccaccat aacctgtttt gaatagaaca acggttccgt
     3661 cctttagtgt ataggtacag ataccggaaa cacagtataa ggtgttgcca gaggcagatt
     3721 ttgatgcgaa agtcgtacta tcgaatgcct cggtctcacc gttgaaggta acaagaaact
     3781 cgtcaccatc gtcgttatat tgaacgacgc cgtagaagtt gtcgccgtat ggtgtagaaa
     3841 agttgctacc cacacgggct aagccgcttt cgccattgcc gattgagatg tctcgcgtgg
     3901 aataggcgac agtaccattt acaagatcga ttccatttcg atcaacggaa gaaatctcag
     3961 aagggcgaac ggcgtctgcg tgggcagagg caaaaggcag ccatgagact gaggttaata
     4021 tcgctgctaa ataagctctc tgaatgcgtt gtacactcat catacccgcc cccaagccat
     4081 ttgaaaaatg ccctgtgaga gcatccccga ttaccatcct tcgcaaga
//
LOCUS       DATMTI010000209         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_348353, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000209 DATMTI010000000
VERSION     DATMTI010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348353"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /locus_tag="VN039_03505"
     CDS             complement(<1..1947)
                     /locus_tag="VN039_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV45084.1"
                     /translation="MNDLLPWSIPSGFLPHGQCLLWESGLIWLHMIADSAIVLAYYSI
                     PFILANFVRKREDLSFGWMLHLFSAFIFACGTTHLMAIWTLWQPAYFVEGLTKVATAG
                     LSIVTAILLVKLVPQALTWPSPAQLEKVNEELRSEIAQRLRVEAELREASDDLKALIE
                     KRTTELAVETVERRRSIEVLRENKAQFKTLAEVAPVGIFHCDRDGFIKYSNERLIEMI
                     GVTSQEEVGTAWGHALHPDDHDRVLAAWAHTVETSQPFRSEHRFVHPDGSVTWVLGQA
                     STVPGENGTVMGFVGTLTDVTEDKAKEEQFRLVVESAPCGMLMVDSEGRITLINAQIE
                     ALFGYSRSELIGCPVEQLIPARFRNTQPEMRETFGHASSVRSMGVGRDLFGLRKDRTE
                     FPVKIGLNPIQTEDDVFVLATVVDITERKHAERQLEQQAAELSRSNHELEQFAYVASH
                     DLQEPLRMVSSYCGLLARRYKGKIDQDADEFIQFAVEGATRMKDLIDDLLTYSRVGRK
                     GAAPVAIEATKSVRAAVANLQIAITEAGAVIHCDSLPTVFADPTQLIQVFQNLIGNAL
                     KFSSERPPVIRISACHLLDAKGSPQWRFSVQDNGIGFDSQYSERVFAIFQRLHTRSEY
                     PGNGMGLAIVKKIVERHGGRIWVES"
     gene            complement(1962..>3252)
                     /locus_tag="VN039_03510"
     CDS             complement(1962..>3252)
                     /locus_tag="VN039_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV45085.1"
                     /translation="ASIGISEIFFTWSTVWDPMWWWWHGVRLFSYGIVAYSAKMDFDK
                     GLVRQTELAMQAEENARFRLIVESAPCGMLMVGSNGTISMVNHQLEVVFGYDRNELIG
                     HPIDNLLPERFRTTPPGIEELHTPTSHQAGVWKRKELFGLRKDGTECPIEVGFSPVEF
                     GSGKQTLVMVLDITERKRAEQHLERQTTELSRSNHELEQFAYVASHDLQEPLRMVSSY
                     CGLLARRYKGRIDQDADEFIQFAVDGAARMKGLIDGLLMYSRVGHREKPLVLVDAGES
                     MQAALANLEIAVKETGASIVCEALPQVLADPVQLTQVFQNLIGNALKFRGNGRPVIHV
                     AARNPGERGNAPKWIFSIQDNGIGIEPQFQERVFEIFQRLHTREEYAGNGMGLAITKK
                     IIERHGGTIWVESTPGSGTIFFFTLRGHVLKRFNAAA"
BASE COUNT          727 a          960 c          806 g          759 t
ORIGIN      
        1 cgattccacc caaatcctgc cgccatgacg ctcgacgatt ttcttgacaa tcgccagtcc
       61 cataccattg cctggatatt cagaccgtgt gtgcaatcgt tgaaagatgg cgaacactcg
      121 ttcgctatac tgtgagtcaa atccgatccc attgtcttga acggaaaatc gccactgcgg
      181 cgagcccttg gcatccagta gatggcatgc cgaaatgcgg atgaccggtg gccgttcgct
      241 gctaaattta agagcattgc cgatcagatt ttgaaatacc tgaatgagtt gagttggatc
      301 agcgaatacg gtgggtagcg aatcgcagtg aatgactgca cctgcttccg taatggcgat
      361 ttgcaaattg gcgaccgccg cccgtacgct tttcgttgcc tcaatggcga cgggggccgc
      421 accttttcgt ccgacccgtg aatacgtcaa caggtcgtcg atcaagtcct tcatccgtgt
      481 ggccccttcg acggcaaact ggatgaactc gtcggcgtct tgatcgatct tccccttgta
      541 ccggcgggcc aaaagcccgc aatagctcga taccatccgc agcggctctt gcaaatcgtg
      601 cgacgccaca taggcaaact gttccagctc gtggttggag cggctcaatt ctgccgcctg
      661 ttgctcgagc tgccgctcgg cgtgctttcg ctcagtgata tctactactg tcgccaggac
      721 aaatacatca tcttctgttt gaatgggatt gagtccgatt ttgactggaa attcggtgcg
      781 gtcttttctc aatccgaaca agtcccgacc tacccccatg gatcgaacac tggaagcatg
      841 accgaaggtc tcccgcattt cgggttgggt attcctgaac cgagccggaa tcaattgttc
      901 aaccggacac ccaatcaact cgctccgcga gtacccgaac agtgcttcaa tttgggcgtt
      961 gatcaaggta atccgccctt ctgaatcgac catcagcatg ccacaagggg ccgattccac
     1021 tacgagccgg aattgttctt cttttgcctt atcttcagta acgtccgtca gcgttccgac
     1081 gaaacccatc actgttccgt tctcgccagg cacagtagag gcttgcccta atacccaggt
     1141 cacactgccg tcgggatgca cgaaccgatg ctccgatcgg aagggttggc tggtttcgac
     1201 cgtgtgtgcc catgcagcaa gaacccgatc atgatcgtca ggatggagag cgtgccccca
     1261 tgccgttcct acttcctcct gtgaagtcac gccgatcatc tcgatcaagc gttcgttgga
     1321 atacttgatg aaaccgtccc tatcgcagtg gaatatgccg acgggagcaa cctccgccaa
     1381 tgttttgaat tgtgctttgt tttcccgcag aacttctatc gaccgcctac gctcgacagt
     1441 ttccaccgcc aattctgtag tgcgcttttc aataagtgct ttcaagtcgt ccgacgcttc
     1501 gcgcaactca gcttccactc gaagtcgttg tgcgatctcc gatcgcagtt cttcattgac
     1561 cttctccaat tgggcagggc tcggccatgt gagagcttgc ggcacaagtt ttaccaagag
     1621 tatcgccgtg acgatggaca agcccgccgt cgccactttc gtcaatcctt caacgaagta
     1681 ggccggttgc catagagtcc atatggccat cagatgggtg gtcccgcacg cgaagataaa
     1741 tgcactgaat aagtgaagca tccagccaaa gctcagatct tcgcgcttcc gcacaaaatt
     1801 ggcgagtatg aatggaatgg agtaatacgc aaggacgatc gcgctgtcag cgatcatatg
     1861 aagccagatc aggccggact cccagaggag acactggccg tgcggaagaa atccggaagg
     1921 gatggaccaa ggcagaagat cgttcattca ccaccttatg ttcaggcagc ggcgttgaac
     1981 cgtttcaaaa catgcccgcg caacgtgaag aagaagatcg taccggaacc tggagttgat
     2041 tctacccaga tagtgccacc atggcgttcg atgatttttt tcgtaatcgc cagccccatg
     2101 ccgttgcccg cgtactcttc ccgtgtatgc aatcgttgaa agatctcgaa cacccgctcc
     2161 tggaattgcg gctcgatgcc gatcccatta tcctgtatcg agaagatcca cttcggtgca
     2221 tttcctcgct cacccggatt tcgagccgcc acgtgaatca ccgggcggcc gttaccccta
     2281 aacttcaacg cattaccgat cagattctga aacacctgag tcagctgtac cggatcggcc
     2341 agcacctgcg gcagtgcctc gcaaacaatc gacgctcctg tttccttgac agctatttcc
     2401 agattggcca gagccgcctg catgctctcg cccgcatcga ccaagaccaa gggcttctct
     2461 cggtggccga cccgtgagta catcagcaaa ccgtcgatca aacccttcat gcgtgcggcc
     2521 ccgtcgacgg caaattggat gaactcgtcc gcgtcctgat cgatccttcc tttgtaccgg
     2581 cgggccagaa gcccgcaata gctcgacacc atccgcaacg gctcttgcag atcgtgcgac
     2641 gccacatagg cgaactgttc cagttcgtgg ttggagcggc tcaattccgt cgtctgtcgc
     2701 tcgagatgct gctcggcgcg tttccgctcg gtgatatcca acaccatcac aagagtttgt
     2761 ttcccggagc cgaactcgac aggactgaaa cccacttcga tcggacactc tgttccgtcc
     2821 ttgcgcaacc caaacaactc tttccttttc caaacacctg cttgatggga cgtgggagta
     2881 tgcagctcct ctatgccagg cggtgtcgtc cggaatcgtt caggcaagag attatcgatg
     2941 gggtgtccga tcagctcatt tcggtcgtac ccgaagacca cttcgagctg atggttgacc
     3001 atcgatatcg tgccattgga tcccaccatc agcatgccgc atggcgcgga ttccacaatc
     3061 aatcggaacc gagcattctc ctccgcctgc atagcgagct cggtttgccg aaccaatccc
     3121 ttatcgaaat ccatttttgc gctgtaggca acaatgccgt aggagaagag tcgaacgcca
     3181 tgccaccacc accacatggg atcccatacg gtcgaccagg taaagaagat ttcggagatg
     3241 ccgatggatg ca
//
LOCUS       DATMTI010000210         4910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_772584, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000210 DATMTI010000000
VERSION     DATMTI010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772584"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VN039_03515"
     CDS             complement(<1..632)
                     /locus_tag="VN039_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWV45086.1"
                     /translation="MSIDMSLDRDAHVLISSPSANRVYRTESKISQQHINITVGPGAI
                     LEWFPEHTIPFAGSRFRQILHATLAPGATLLLWDAVASGRIAREERWAFTDLENEIRI
                     TTASGSSLLERYVLDPTTDLGCVGLAEEWNYVASFYVVNDVVASDTWSRLESRIAPIL
                     DERPGHVLGGVSTPTVPGVAIKLQTQTAPDLTYMLDTLWATIREELWNLPP"
     gene            complement(941..1612)
                     /gene="ureG"
                     /locus_tag="VN039_03520"
     CDS             complement(941..1612)
                     /gene="ureG"
                     /locus_tag="VN039_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HWV45087.1"
                     /translation="MHDINKEHRHDWTPTQARVKGIPVIGIGGPVGSGKTALVEALCQ
                     RLRDRYSLAAVTNDIFTKIDAEILTKRAALPVDRILGVETGGCPHTAIREDASHNQEA
                     IDDLLRRHPDVELIFLESGGDNLAATFSPELVDSVIYVIDVSGGDKIPRKGGPGITRS
                     DFLVINKMDLAPYVRADLSVMDRDTRRMRGDRPFAFTNILSGEGLDAVVAWMEQRIPQ
                     RARRS"
     gene            complement(1695..2423)
                     /locus_tag="VN039_03525"
     CDS             complement(1695..2423)
                     /locus_tag="VN039_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HWV45088.1"
                     /translation="MTVVGRLFYSCVPDMNTPALLEGLRFIDTFFPSGGYAFSSGLEA
                     AIQGGAVKNSDQLARYVGDLLRGGMSRREAFAVKWANQAGSDGNVSLAVDVDRELDST
                     KVSRESRMASRQMGRQVMRVAADQLRTKTVLGEYRDAIESERAPGHLAVCFGLTLGVS
                     GWEPEATVAAFLYQTAIGFVSAAMRLSPIGQHEGQRILSEWLPLIDRISRETDSDAAM
                     SSWSPIQDIYSMRHGRLEWRLFRS"
     gene            complement(2463..4187)
                     /gene="ureC"
                     /locus_tag="VN039_03530"
     CDS             complement(2463..4187)
                     /gene="ureC"
                     /locus_tag="VN039_03530"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWV45089.1"
                     /translation="MKISRKQYASLYGPTVGDRVRLGDTDLIIEVEEDRIIPGEEAVF
                     GGGKTIRDGMGQSARATSTSGQLDTVITSALILDYTGVIKADIGIKDGRIVGIGHAGN
                     SDLMPNVTPGMEIGPGTEAISGEGRIITAGGIDTHIHFICPQQCWEALSAGLTTMIGG
                     GTGPSNGTSATTCTPGPWNIHRMLEASEGIPINLGFLGKGNASRPEGLNEQIEAGAIG
                     LKLHEDWGTTPAAIDTCLSVAERYDVQVAIHTDTLNEAGYIEDTIRAIKGRAIHSFHT
                     EGAGGGHAPDIIKICGEPNVLPSSTNPTMPFTVNTMDEHLDMLIVCHNLNPRIPEDVA
                     FAESRIRRETIAAEDILHDMGAISIMSSDSQAMGRIGEVITRTWQTAHKMKLQRGHLA
                     SVSGKSSTQNDNFRARRYVAKYTINPAITHGIAHEVGSVEVGKIADLVIWKPEFFGVK
                     PEMVLKSGFIAQAQMGDPNASIPTPEPTISRPMFGAFGRALSSTSFTFVSQAAVDHEI
                     PKRLGLQRRVSAVKNCRHLGKRDLKLNDSLPKVEVNPETYVVTADGVPLICEPAIVLP
                     LAQRYQLF"
     gene            complement(4184..4636)
                     /locus_tag="VN039_03535"
     CDS             complement(4184..4636)
                     /locus_tag="VN039_03535"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HWV45090.1"
                     /translation="MKKHDAPQRSKEALAAAVKAELSAPIVPGEIMTVAGDIEIFKGR
                     ETKEVTIKNVGDRPIQVGSHCHFFETNRALKFEREQAFGFRLAIPAGTAVRFEPGEEK
                     KVTLVALSGNRLAQGVNGLTDGLLDDPKVKAKAVSLAAERGFGKGGAR"
     gene            complement(4669..4824)
                     /locus_tag="VN039_03540"
     CDS             complement(4669..4824)
                     /locus_tag="VN039_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45091.1"
                     /translation="MTRKNKALPRKPEHRGGSFSRTVDVLRQELERYHYWETLGNKAL
                     HMLRMNR"
BASE COUNT         1023 a         1490 c         1274 g         1123 t
ORIGIN      
        1 ggtggaagat tccacagctc ttcacggata gttgcccaga gagtatcaag catgtaggtg
       61 aggtcgggtg ccgtttgagt ctgcaatttg atggcgaccc ccggcacagt cggcgtcgat
      121 actcctccca acacgtggcc cggccgttca tccaagatag gcgcgatcct cgactccagt
      181 cggctccaag tatcggacgc gacgacatcg ttgaccacat agaaggaggc aacatagttc
      241 cattcttctg caagccctac gcaacccaaa tcagtagtcg gatcaagaac atatcgctcc
      301 agaagagaac tgccggatgc cgttgtgata cggatttcgt tctccaggtc ggtaaacgcc
      361 catcgctcct ccctggcgat acgcccggat gcgacggcat cccacaagag aagagtcgca
      421 ccaggcgcca gcgtagcatg aagtatctgc cgaaatcgtg acccggcgaa cggaatggtg
      481 tgttcaggga accattccaa gatcgcacca ggcccaacgg taatattgat gtgctgttgg
      541 gagattttac tctcggttcg atagacccga ttggcggacg gtgaggagat caggacgtgc
      601 gcgtctcgat ccagactcat gtctatagac aaatggtccc ctccgaccag gcctccggat
      661 ggattgacga gcagcgtgta tgcggccccc gtatcatcaa gataaatggg cggaaggagc
      721 ttccatggcg tcgtgaagta cgagtgtgcg atgatagttc ggcggtcggg cttggcatag
      781 tcaagccgaa gctcaccgac ccgtccgata aattcggtgg aaaacctggg cgacgtactt
      841 ctttttctgg aggggctcct tttttcagaa gtgtttcgtt tcggagccgt acgtcttcgc
      901 gaagcagcct tttttcgtgg tcttgttgat gcctgcacga ttatgagcgc ctcgctcgct
      961 gcggtatgcg ctgctccatc cacgccacca cggcatccag cccttcgccg gaaaggatgt
     1021 tggtgaacgc gaagggacgg tcgccacgca tccggcgggt atcccgatcc atgacagaca
     1081 gatccgcgcg gacgtacggc gccaaatcca tcttgttgat gacgagaaaa tccgatcgag
     1141 taatgccggg accgcccttg cgtgggattt tatcgccacc cgatacatcg atcacataga
     1201 tgacggaatc cacgagttcc gggctgaagg tcgccgcgag attgtcccct ccgctttcca
     1261 gaaagatcaa ttcaacatca ggatgacgcc gaagcaggtc atcgatcgcc tcttgattat
     1321 gcgaggcgtc ttctcggatg gccgtgtggg ggcacccacc cgtctccacg cctaaaatcc
     1381 gatcgaccgg caaggctgca cgcttcgtca gaatttccgc atcaattttt gtaaaaatgt
     1441 cgtttgtcac agcggctaaa ctgtaccgat cccgcaatct ctggcatagg gcctcaacga
     1501 gcgccgtctt gcccgatcct accggccccc cgatcccgat gaccggaatg cctttcaccc
     1561 ttgcctgagt gggtgtccag tcgtgacgat gttccttgtt gatgtcatgc atggcaacct
     1621 cgaagggttc agggcctatc gccctggcat caaccttctt ttccctttct gttatgaatg
     1681 atagttgtca attctcaaga ccggaagagt ctccactcca accgaccatg gcgcatcgaa
     1741 tagatatctt ggatgggaga ccacgagctc atcgctgcgt ccgagtcggt ttctcgactt
     1801 atgcgatcga tcagtgggag ccattcactg agtatgcgct gtccttcatg ctggccgatc
     1861 ggactcaacc tcatggcggc agacacaaac ccgatagccg tttggtacaa gaaggccgcc
     1921 acagtcgctt cgggttccca tccactgaca cccagtgtca gtccaaagca gaccgccaga
     1981 tgcccgggcg ctcgttcgga ttctatcgca tcacggtact cgccgagaac cgtcttggtc
     2041 cggagctgat ccgccgccac gcgcatgacc tgacgaccca tttggcgact agccattcgc
     2101 gactcacgac tcaccttcgt cgaatccaac tctcgatcga catcgacagc cagagagaca
     2161 tttccatcgg atccggcttg attcgcccat tttaccgcga aagcttctct gcgactcatg
     2221 ccgccacgca acaggtcccc cacgtagcgg gccagttgat cggaattctt caccgctccg
     2281 ccctgaatgg ccgcttccaa tcccgaggaa aacgcatagc cgccggatgg gaagaatgta
     2341 tcgatgaacc tcaacccttc aagtaatgcg ggtgtattca tatcgggcac acaagaataa
     2401 aacagccggc caactaccgt cacgatctct cgtgactgat aatcgagcca gctgtcccgt
     2461 cgttagaaca attggtaccg ttgcgccagc ggcagcacaa tcgccggctc gcagatgagc
     2521 ggcacaccgt cagcggtcac aacgtacgtc tccggattga cttcaacctt cggcagcgag
     2581 tcgttcagct tgagatcacg cttccccaag tggcggcaat ttttcaccgc agacacgcgc
     2641 ctctgcaaac cgagtcgctt cggaatctca tgatccacag ccgcctgaga cacgaaggta
     2701 aaactggtgc tggacaatgc gcgaccgaat gcgccgaaca tcggtcggct gatggtcggc
     2761 tccggcgtcg ggatcgaggc gttgggatcg cccatttgag cctgggcgat gaagccgctc
     2821 ttcaatacca tctcaggttt cacgccgaaa aactccggtt tccagataac gaggtcggcg
     2881 atcttcccga cctccaccga accgacttca tgggcgatgc cgtgcgtgat ggccggattg
     2941 atcgtgtact tggccacata ccgtctggcc cgaaagttgt cgttctgggt agaagacttg
     3001 ccgctcactg aggcgagatg ccctcgctgt aatttcatct tatgcgcggt ctgccacgtt
     3061 ctggtaatga cttccccgat gcggcccata gcttgggagt ccgatgacat gatgctgatc
     3121 gcccccatgt catgaagaat gtcttccgca gcaatagtct cacgtcggat gcgggactcc
     3181 gcgaacgcga cgtcttccgg aatccgcggg ttcaagttgt ggcacacgat caacatatca
     3241 agatgctcgt ccatcgtgtt gacggtaaac ggcatcgtgg ggttagtcga agacggcagc
     3301 acgttcggct ctccgcaaat tttaatgatg tccggggcat gaccgccgcc tgcaccttct
     3361 gtatggaagc tatggatcgc tctccccttg atggccctga tggtatcctc aatgtaaccg
     3421 gcctcattga gcgtatccgt atggatcgcc acctgtacat cataacgctc tgcgacgctc
     3481 aagcaggtat cgatcgccgc cggggtagta ccccaatctt catgcagctt caacccgatg
     3541 gcgccggctt caatttgttc gttcaacccc tcgggtcgtg acgcattgcc cttccccaag
     3601 aaaccgagat tgatcggaat gccctcggac gcttccagca tgcgatgaat attccacggc
     3661 cccggcgtac aggtcgtggc actggtccca tttgaaggac cggtcccacc gccgatcatc
     3721 gtcgtcaggc cggccgacag cgcttcccaa cattgctgcg gacagatgaa gtggatatgc
     3781 gtatcgatac cgccggccgt gatgatccgg ccttctccgg aaatcgcttc cgttcctgga
     3841 ccgatttcca tgcccggcgt gacattcggc atcaggtctg aatttccggc atgaccgatg
     3901 ccgacgatcc ggccgtcctt gatgccgatg tcggccttga taaccccggt atagtcaagg
     3961 atgagcgcac tagtaatcac ggtgtcgagc tggccgcttg tgctcgtagc ccgagcagac
     4021 tgccccatgc cgtcgcgaat ggtcttgccg ccgccgaaaa ccgcttcctc tccgggaatg
     4081 attcgatctt cttcgacttc gatgatgagg tccgtgtcgc cgagccggac acgatcgccg
     4141 accgtgggac cgtacagaga tgcatattgc ttgcgtgaga ttttcaccgt gcgcctcctt
     4201 ttccaaatcc gcgttccgca gcgagcgaaa ccgccttggc tttgaccttc ggatcatcca
     4261 acaatccatc ggtcaacccg ttgacaccct gggcgagccg gttacccgaa agagccacaa
     4321 gcgtcacctt cttttcttcc cccggctcaa accggacggc cgtacctgct ggaatggcaa
     4381 gacggaaacc gaacgcttgc tcacgctcaa acttgagcgc gcgattggtc tcaaagaagt
     4441 gacagtgaga tcccacctga atcgggcgat cgcccacatt cttaatcgtc acttctttcg
     4501 tctctcgccc cttgaagatt tcaatgtcgc cggccaccgt cataatctcg ccgggcacga
     4561 tcggggcgga caactcggct ttcaccgcag ccgctaaggc ttctttcgat ctctgtggcg
     4621 cgtcatgttt tttcattttc gctcctcctg ccccaccgac ctttgtgatc agcgattcat
     4681 tcgcagcata tgcaatgcct tgttgcccag cgtttcccaa tagtgatacc gctccaattc
     4741 ctgccgcaac acgtccactg tgcgcgagaa actgccgccg cgatgttccg gttttctcgg
     4801 caacgctttg tttttccttg tcatctccag aatcctgtgg gtttttcgct ttcatattac
     4861 ttcactcctt tttcgtgctc tgtgtcggtc taaacaattc cccatcttca
//
LOCUS       DATMTI010000211         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_306144, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000211 DATMTI010000000
VERSION     DATMTI010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306144"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VN039_03545"
     CDS             complement(<1..239)
                     /locus_tag="VN039_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45092.1"
                     /translation="MLNALELKVPPLALVFLFGALMWLVPAYSVFTIALPWRSALAFI
                     FYTGGSAIVLAGILTFRRMKTTVNPLTPEATTTMVT"
     gene            complement(266..766)
                     /locus_tag="VN039_03550"
     CDS             complement(266..766)
                     /locus_tag="VN039_03550"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HWV45093.1"
                     /translation="MSQVTGIGLSFRVLLLLLASCADQSYQVRQPGGYGEMPPGETWV
                     PVDSAYQSPSREMVYYDPKTIRRDGDLVTLWQLTDYKWMQGNAPFGTFMMSPHRFFST
                     KTHKEFDCAHNRVRLLASSEFSQHMGTGTHSAVLVSQGSGHPVEPGSINQALWEVACG
                     VTNTPS"
     gene            complement(887..1153)
                     /locus_tag="VN039_03555"
     CDS             complement(887..1153)
                     /locus_tag="VN039_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HWV45094.1"
                     /translation="MHVTPKKNRGRVDIIRQTAHRGGKTVTVVTGFIGIGQSEKERLA
                     KQMQHVCGAGGTVKDGRIEIQGDQRGVVARILIEAGFRPIFAGG"
     gene            complement(1199..1720)
                     /locus_tag="VN039_03560"
     CDS             complement(1199..1720)
                     /locus_tag="VN039_03560"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV45095.1"
                     /translation="MMSIWRGVFAMLLILACTSGAGPESVVATQSLEEAEFAYERGDY
                     TQAARFFSPLAEQGVASAQFYLGLMYEKGRGVRQDYSAALGWFRKAAAQGYAGPQNNL
                     GLMYERGRGVRKDSVRALMWYTVAVAMLNGDEGRTAVKRLDHLTSQMTAAQIEQAQEM
                     ARRCQQSQFKECE"
BASE COUNT          450 a          570 c          561 g          422 t
ORIGIN      
        1 gtgaccatcg tcgtggtcgc ttccggcgtg agtggattga cggtggtttt catccgacga
       61 aacgtcaaga tgccggccag cacgatcgct gagcctcctg tgtaaaaaat aaaggcgagc
      121 gccgaacgcc atggcaaggc aatggtgaat acagaatagg cagggacgag ccacatcagc
      181 gcgccgaaca ggaagacgag cgcaagcggc ggtactttga gttccagtgc attcaacatg
      241 tgcgtcggga cgggtaaggt caggctcaac tgggggtgtt ggtcacccca caggccactt
      301 cccaaagtgc ctggttgata ctgcctggtt ccaccgggtg cccagagcct tggctgacca
      361 ataccgcact gtgagttccg gtgcccatat gctgagagaa ttccgaagac gccagtagtc
      421 gaacacggtt gtgtgcgcag tcaaactcct tgtgcgtctt cgtcgagaaa aaccggtgag
      481 ggctcatcat gaatgtccca aacggcgcat tcccttgcat ccacttatag tctgttaact
      541 gccacagcgt cacgagatct ccgtctcggc gaatcgtctt gggatcgtag tacaccattt
      601 cccgcgatgg agattggtac gcgctatcga cgggtaccca ggtttcccca ggcggcatct
      661 ccccatatcc tccgggttga cggacctgat aactctgatc tgcacaggac gcaaggagga
      721 gaaggaggac acggaaagaa aggccgatac cggtaacctg ggacatagat tcctcgccgg
      781 ataatgcatt ctagcaaagg gtgaaaagga aggtgcaagg gctgtttgcg gtgtgtctgt
      841 aggtagtctg cgaaagacaa gggacagctg agcttgcgtc agggaattag cctccggcga
      901 agattggacg aaatccggcc tcgatgagaa tgcgggcgac cacgccgcgc tgatctcctt
      961 gaatctcgat ccgcccatct ttaaccgtgc ctcccgctcc acagacatgc tgcatctgct
     1021 tggccagccg ctctttctca gactgcccga tcccgataaa ccctgtgacc accgtgaccg
     1081 tcttgccgcc gcgatgcgct gtctgacgga tgatatccac ccggccacga ttcttcttag
     1141 gcgtgacgtg cattgggttg gtctgatcga aatcagatgg tgtgtgcgaa ggccgctcct
     1201 actcgcactc tttaaactgt gactgctgac aacgccgagc catctcctgc gcctgctcga
     1261 tctgtgcggc ggtcatttgt gaggtcaggt ggtctagccg tttcacggct gtccttccct
     1321 catcgccatt caacatcgcg acagcgacgg tgtaccacat aagcgcgcga accgagtctt
     1381 tccggacacc ccgtcccctc tcgtacatca ggcccagatt gttctgcgga ccagcgtagc
     1441 cctgcgccgc cgccttacga aaccacccta gtgccgcgga gtagtcctgc cggacgcctc
     1501 gtcctttttc atacatgagg cccagataga actgggctga tgctactcct tgctccgcca
     1561 ggggactgaa gaaacgggcc gcttgtgtat aatcgccacg ctcatacgca aattccgcct
     1621 cctcaagaga ctgtgttgca acaacagact ctggtcccgc ccctgacgtg caggccagaa
     1681 tcaaaagcat tgcaaagaca ccacgccata tcgacatcat cctttcacca agatctatca
     1741 ccacaggcga gcattgcctc aacgctcgga aggaaacaga gaataactca ttctactgtg
     1801 tgaagtccgg cgggtctatg gatttgaaga gggaggcata tcgagagggg agggtgggag
     1861 gcgggacgga acttctagca aacaccatag atgacgtccc cacagaaagg agagagcggc
     1921 ctcctgttag gatctcttag cgtgcggcaa cggtctggga ttctcgggca gccaggctct
     1981 ttcggcgatt attggaaatg gtg
//
LOCUS       DATMTI010000212         5668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_815389, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000212 DATMTI010000000
VERSION     DATMTI010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815389"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VN039_03565"
     CDS             <1..1197
                     /locus_tag="VN039_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWV45096.1"
                     /translation="RTIRDLLAQDRAGERILLRAAQRRNRTLGGDHVRLYKNNTDFHG
                     HSYGCHDNYLVSRAVPFPALVAGLVPFLVSRQVIAGAGKVGTEAQESGYVPGRYQLSQ
                     RADFMEAELGIDTMHNRPILNTRDEPHADRRKYRRLHLIIGDANMCEYATALKVGTTR
                     LVLDLIERGAMPHIELEQPVVAVKQLSRDPDLKAVVRLQDGRRLSALELQGYYCEAAR
                     RELAGSGEESDWLLAEWSETLHLLDRDRSQLVGKLDWVTKQWLLETFMREERIGWDDP
                     WLASLDLEYHNVDPEQGLYLGLEADGKAWRMTTDEAIDEALLNGPNDTRGGLRGLCVQ
                     KFADKINSMQWEWIQFKDGLRSRTLDMADLFDPQEVRRCVDVFQRAYSPADALAAWSH
                     RKDTET"
     gene            1194..1388
                     /locus_tag="VN039_03570"
     CDS             1194..1388
                     /locus_tag="VN039_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HWV45097.1"
                     /translation="MTHSMMPERREGPVDPMPKSPRPQEEGGGPRRPDTGSPDKDNLM
                     KRMRKVDPKQAERYRQRTGE"
     gene            1477..2250
                     /locus_tag="VN039_03575"
     CDS             1477..2250
                     /locus_tag="VN039_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HWV45098.1"
                     /translation="MGMQGDFYQLLKEQGYAFGASGQAGARQELTTATTILAFKYREG
                     VLVAGDRRATAGNMVMYDRTDKVLEIDRYSVMAIAGVPATAYEMARILEHSFKYYRRT
                     QLQELSFEGKLRALSKLLKENVAAALAGTGAVAPIFAGYDAGQEAAKIYFYDILGAEF
                     EGVEYAVSGSGSSTIRGILHYLNTWGEQPLSAMPEEQATVQALRLLTSAAEFDSATGG
                     VNREAGLYPVIKFITADGVRMMPDLQLRSLFESGVSRSA"
     gene            2263..3072
                     /locus_tag="VN039_03580"
     CDS             2263..3072
                     /locus_tag="VN039_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45099.1"
                     /translation="MYEEPYRWVEAVGNRRQYLDDQLKEGTPVVGIAYERGILLMTMS
                     KGTPKLYEIYDRLALGGMGHPADLEKLRFNLLEMAHVEGFNRSASDVTGSRLVKYGIA
                     PVIKQAFEEVFKAPFIVKILLAEVGRKTGKDGFLTINYDGTFEEKNQYAVLAATSVVE
                     QKMMGYLRQQSIVSLKDAVDAAIRTWAVGDQAQQPAHEDDESHEEAKTSPDQQVIDAK
                     ILYAHLRRRIAEKTIECALLDRQVQGPSKYRALEADELRTMLPADLSPSLA"
     gene            3077..4564
                     /locus_tag="VN039_03585"
     CDS             3077..4564
                     /locus_tag="VN039_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWV45100.1"
                     /translation="MLHRIFGLETEYGLLIHEDRPNHSPTWFAHKIRDHLFQVQRRGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYLDMGHLEYASPECHSLTDVVASDRAGDFLLQQA
                     LEELGFSEQVSLIKNNIDHETDATFGSHENYLVSRRFPFSRRGLAPFVTFLVTRQIFT
                     GSGRVGSAAPQDAWIQTDRLILPRSALGAQHVSDLPFQISQRADYIVNDFFEWVQHNR
                     AIVNTRDEPLADPNQYRRIHLLLGDSNMAEYATALKLGTTGLVLQLIEEGHAPQDLEI
                     DEPVEALQEISQDQERRWLIQLQSQKTMSALDIQERFLDCAVRHCKGQDEETDWVLAQ
                     WASVLHDLRGDYQKLVGRVDWASKLWLLECFREAEHVDWANPMLKSLDLEYHNLNHAK
                     GLYYGLLEEGRVPRVTTDRLIHLATETAPKNTRAYGRSELVRHLLHELPASAADQSPA
                     PDGLFPPYVINWSIFQVRGHAPFPMPDPFKTYLEDVRTHLKSVIV"
     gene            complement(4609..>5668)
                     /locus_tag="VN039_03590"
     CDS             complement(4609..>5668)
                     /locus_tag="VN039_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV45101.1"
                     /translation="SKTCGEFFNVSAPCIILNMVADPAIVDIASSFQPPFLLRNAHLM
                     TLVPRYVPRDTSLAGLPQESRLFSVDPDAQLQGFCHWQDDRKASPTAILVHGLEGCSE
                     SRYMRGIAAKAYRAGFNVIRMNQRTCGGTEHLSPTLYNSGLSGDYRSILTELAHQDGL
                     DHLWLVGYSMGGNLILKAGGELGRTEPALAGIAAVCPNINPTVCARALEESRNFIYHR
                     HFLARLKSRLRRKAALLPGKWDLSQLDRIATISEFDDRYTAHDGGYRDGADYYDRAGA
                     RHVLDTIAVPTLIITAQDDPFIPYSMFTVPKIQRHPYIRVVAPLYGGHCGFFHRSRNG
                     EDPYWAENRIVDFLLGRL"
BASE COUNT         1311 a         1434 c         1704 g         1219 t
ORIGIN      
        1 cggacgatca gagatcttct tgcccaggat cgcgcgggag aacgtattct tctgcgagct
       61 gcgcagcgac gcaatcgaac gctcggagga gatcacgtca gactgtacaa gaacaatacg
      121 gatttccatg ggcacagtta tggatgtcac gacaactatc ttgtctcgcg ggccgttccc
      181 tttccggctc tcgttgccgg cttggtgccg ttcttggtga gccggcaagt gatcgcgggt
      241 gcgggaaagg tcgggactga agcacaggag tctggttatg ttccaggacg gtatcagctc
      301 tcgcagcgcg ctgattttat ggaagcggag ctgggtatcg atacgatgca taaccgaccg
      361 attcttaaca caagggacga gccacatgcc gaccgccgaa aatatcgccg actccatctc
      421 atcatcggcg atgccaacat gtgcgagtat gcaacggcgc tgaaggtcgg gacgaccaga
      481 ctcgttcttg atttaatcga gcgaggggct atgcctcaca tcgagctgga gcagccggtg
      541 gttgcggtga aacagttgtc gcgcgatccg gacctgaaag cagtcgttcg tctccaagat
      601 ggacggaggc tttctgctct ggagcttcaa gggtattact gtgaggcggc acgccgcgag
      661 ttagccggat ccggtgagga gtcggattgg ctgttggcgg aatggagtga gaccttacac
      721 ctgttggatc gagaccgctc gcagttggtg gggaagctgg attgggtcac aaaacagtgg
      781 ttgctcgaga cattcatgcg agaagagcgg atcggttggg atgatccctg gttggccagt
      841 ttggatttgg agtatcacaa tgtggatccg gaacaggggc tatatctcgg cttggaagcg
      901 gatgggaagg cttggcggat gacaaccgac gaggcaattg acgaggcgct tctgaatggc
      961 ccgaacgata cccgtggtgg cctgcgtgga ctctgcgtgc agaaatttgc ggataagatt
     1021 aactcgatgc aatgggaatg gattcagttc aaagacgggc ttcggtcccg tactctggac
     1081 atggcagatc tctttgatcc acaagaggtg cgacgatgtg tggacgtctt ccagcgcgcc
     1141 tattcaccgg ctgatgcgct ggcagcgtgg agccacagaa aggatacgga gacatgacac
     1201 acagcatgat gccggaacga cgcgagggcc cagtcgatcc catgccgaag tcgccccgtc
     1261 ctcaagaaga aggaggcgga cctcggcgac cggataccgg atcgccggac aaggataacc
     1321 tcatgaagcg gatgcgcaag gtcgatccca aacaagcaga gcggtatcgg cagaggaccg
     1381 gggaatagtt ctgagtgctg agcagaacgc aaacttagga gacgatccgc tttaactact
     1441 cagcacccag catttcaaac tcagcactga ggaacaatgg gcatgcaggg agacttctat
     1501 cagctcttga aagaacaagg atacgcgttc ggagcgtcgg ggcaggccgg agcaagacag
     1561 gaactgacga cagccacaac catccttgcc tttaagtatc gcgaaggcgt tctggtcgca
     1621 ggggatcggc gcgcgacagc cggaaatatg gtgatgtacg accgcaccga taaggtattg
     1681 gagatcgatc ggtatagtgt catggcgatc gccggagtgc cggcgaccgc atatgaaatg
     1741 gcgcgcatcc ttgaacattc tttcaagtat tatcggcgga cacaactgca ggaactcagc
     1801 tttgagggga aactgcgagc tctgtccaaa ttgctgaagg agaatgtcgc ggcggcctta
     1861 gcggggactg gagctgtagc accgatcttt gccggttacg atgccgggca agaggcagcg
     1921 aagatctact tttacgatat tctcggcgct gagttcgagg gagtggaata tgcggtatcc
     1981 gggtcggggt cgtcgaccat tcgtgggatt cttcattact tgaatacatg gggtgagcaa
     2041 ccgctgagtg cgatgccgga agagcaagca accgttcaag cgctgcgatt gttgacgagt
     2101 gctgcagaat tcgacagcgc gaccggtgga gtcaaccgcg aggccggtct ctatccggtc
     2161 attaagttca tcacggccga cggtgtcagg atgatgccgg atcttcagct ccgatcgctg
     2221 tttgaatcag gagtttctag aagcgcgtaa ggatcattcg ctatgtatga agagccatat
     2281 cggtgggtgg aagcagtcgg aaatcgacgg caatatctcg acgatcagct taaggaggga
     2341 actcctgtcg tcgggatcgc gtatgagcgt ggcattctgc tcatgacaat gagcaaaggc
     2401 acgcccaagt tatatgagat ttacgatcgc ttagccttgg gaggtatggg acatccggcc
     2461 gacctggaga agctgcggtt caacttattg gagatggctc acgtcgaagg gttcaatcgc
     2521 tccgcttccg atgtgaccgg gagccgcctg gtgaagtacg gcatcgcgcc tgtcattaaa
     2581 caagcgttcg aagaggtgtt caaggcacct ttcatcgtga aaatcttgct ggcggaagta
     2641 ggccgaaaaa ccgggaaaga cggtttcctg acaattaact acgacgggac ttttgaggaa
     2701 aaaaaccagt atgcggtgct tgcggctaca tcggtggtgg agcagaagat gatggggtat
     2761 ctccgacaac agtccattgt gtctttgaag gacgctgtgg atgcggcgat tcgtacgtgg
     2821 gcggtcggtg atcaagcgca acagccggcc cacgaggatg acgaaagcca tgaggaagca
     2881 aagaccagcc ctgatcagca ggtgattgac gcgaagatcc tctacgcgca tctgcgtcgg
     2941 cgcattgccg aaaagacgat cgaatgcgcc ttgcttgacc gccaggtgca aggaccttcc
     3001 aagtatcgag cacttgaggc cgatgagttg agaacaatgc ttccggcgga tctgagccca
     3061 tcactcgcgt aatctcatgt tacatcgtat ctttggtttg gagactgaat acgggctttt
     3121 gattcacgag gaccgaccga accattcccc gacatggttt gcccataaga ttcgtgatca
     3181 tctgttccag gtccagcggc ggggtgtgct ggacttgcac catcgtgggc atgatgagcc
     3241 ccccggcaat gggggatttc tcaccaacgc cgggcgaatc tatttggaca tggggcatct
     3301 agagtatgcc tcgccggaat gtcattccct cacggatgtg gtggcgtcgg accgtgccgg
     3361 ggacttcttg ctgcagcaag ccctcgaaga actcggcttc tctgaacagg tgtccttgat
     3421 caagaacaac atcgatcatg aaaccgacgc gactttcggc tcgcatgaga actatctggt
     3481 ttctcgacgg tttccttttt cgaggcgcgg gctggctccg ttcgtgacgt ttcttgtgac
     3541 caggcagatt tttaccggct ctggccgtgt cggttccgcc gctccacaag atgcctggat
     3601 ccagacggat cgattaatcc ttccacggtc ggctctcggc gcgcagcatg tatcggattt
     3661 gccctttcaa atctcccaac gtgccgacta tatcgtgaac gatttcttcg aatgggtgca
     3721 gcacaatcgg gcgattgtga acacgagaga tgagccgttg gccgatccca atcaataccg
     3781 aaggattcat ctgctgctgg gcgattccaa catggccgaa tatgcgacgg cattgaagct
     3841 tggaacgacg gggctggtgc tgcagctcat cgaggaagga catgcgcctc aggatttgga
     3901 gatcgacgaa ccggtggagg cgctccagga gatttcccaa gatcaagaac ggcgctggtt
     3961 gatccagtta cagtcccaaa agacgatgtc ggctttggat attcaagaac ggtttcttga
     4021 ctgtgccgtt cggcattgca agggacagga tgaagaaacc gattgggtcc ttgcccaatg
     4081 ggcgtcggtg cttcatgacc tgcgcggcga ttatcaaaag ctcgtcgggc gtgtcgactg
     4141 ggcatccaag ctgtggctgc tggaatgctt tcgagaagcg gaacatgtcg actgggccaa
     4201 tcccatgctg aaaagtttgg atttggagta tcacaatctg aatcacgcga aggggctgta
     4261 ttacggctta ttggaagagg ggcgtgtgcc tcgcgtgacg acagaccgcc tcatccatct
     4321 tgcgacggaa acggcgccga aaaacacgcg tgcctacggc cgcagtgaac tggtcagaca
     4381 tctcctgcat gagctgcccg cctcggcggc ggatcagtct ccagcgcctg atgggttgtt
     4441 ccccccgtat gtcattaatt ggtcgatctt tcaagtgcgg ggtcatgccc cgtttcccat
     4501 gccggatccg tttaagacct atctggaaga tgtgcgaaca cacctcaaaa gcgtgatcgt
     4561 ttagtcagtc aattcttcat gcggcaggca agcttgacgc ggcgtccgtc atagccgccc
     4621 cagcaaaaag tctacgattc ggttttccgc ccagtacggg tcttctccat tccggctccg
     4681 gtgaaaaaac ccgcaatggc ctccgtagag aggcgcgacc acacggatgt aaggatgccg
     4741 ctgaatcttc ggtacggtga acatcgagta gggaataaac ggatcatctt gcgccgtaat
     4801 gatgagagta ggaacggcaa tggtatcgag cacatggcgt gcgcccgccc gatcatagta
     4861 atcagcaccg tctcgatagc cgccgtcatg agcggtatag cgatcatcaa attcgctgat
     4921 cgtcgcgatc cggtcgagtt gggaaagatc ccatttccca ggcaacaggg ccgcctttct
     4981 ccgcaacctc gatttcaacc gcgccaaaaa gtgccgatga taaatgaaat tgcgagattc
     5041 ctccagtgca cgagcacaaa cagtcgggtt gatgttcggg cacactgctg cgattcctgc
     5101 tagagcgggc tcggtccggc cgagctcgcc gcccgctttc aagatgagat ttccgcccat
     5161 ggaataccca accaaccata ggtggtctag tccgtcctgg tgagcgagct ccgtgagaat
     5221 cgagcgatag tctccgctca agccgctgtt ataaagggtc ggggagagat gctcggttcc
     5281 gccgcaggta cgctgattca tgcggatgac attgaagcct gcacgatagg cctttgcggc
     5341 aatgccgcgc atgtatcgcg attcgctgca gccttcaaga ccgtggacca gtatcgctgt
     5401 gggagaagcc ttgcgatcgt cttgccaatg acagaatccc tgaagctgtg catccggatc
     5461 aacggaaaat aaacgcgact cctgaggcaa tcctgccaag gacgtatccc gcggcacata
     5521 gcgagggaca agcgtcatga ggtgagcgtt ccggaggagg aacgggggct gaaatgacga
     5581 ggcgatatcc actattgcag gatcggccac catattgagt atgatacacg gagctgatac
     5641 gttgaagaac tctccacagg ttttcgag
//
LOCUS       DATMTI010000213         5943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_306170, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000213 DATMTI010000000
VERSION     DATMTI010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306170"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VN039_03595"
     CDS             complement(<1..225)
                     /locus_tag="VN039_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45102.1"
                     /translation="MNTLCRSLSHTIIVALLLFQAGCGATQLGMCAPHTKEEGTPTRN
                     WVECFDQPFAHGGMTHSVFCLHDGTDKPPVL"
     gene            386..1510
                     /locus_tag="VN039_03600"
     CDS             386..1510
                     /locus_tag="VN039_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWV45103.1"
                     /translation="MITETEGPLSIPSTPESISPTWLTTTLRRAGVLDRAAVVGITVT
                     PVAGGSGFVGQTARLRVAYDAREAGAPATVFAKLSSADPAVRQQLRRVGIYETEAGFY
                     RDMALQPTFPIRVPRPYLSQYDEDTSASILLLEDLSAVEFGDNLTGCSATDARIAVRQ
                     LGLLHAHCWNAPLLKSLSWLRALTDEVQARAGMYQAMLPRFEQRCAEFLTPALREAAR
                     RFALVFPEYSQQCSNGPQTLTHGDFRADNLAFVQTSEGRGVTVFDWQVVRRSRGPRDL
                     AYFLSSSLSVEQRRTLEESLLDLYDETLKANGVEGYSTGDLRRDIQAGLGAPLTTWVI
                     AGGMLDFSSERGTDLLKQVCARLGAALDDHRFMSYLDELA"
     gene            1535..2344
                     /locus_tag="VN039_03605"
     CDS             1535..2344
                     /locus_tag="VN039_03605"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV45104.1"
                     /translation="MALKQHEIYSAGYDPLVLQGLARRSATRDAAFFVPHLTPVMHVL
                     DCGCGPGGISVTLAALVPQGRVVGVDIEDRQLDMGRREAQQRGIGNVEFHHASLYALP
                     FADESFDAVLAHAVLYHLAEPMNALRELWRILKPGGVIGLRDADFDGDVYYPAHEDVD
                     RFWKLTMRVIEHNGGDPRFGRKQRRLLREAGFRNIAGSASSDAFGTLEMTAGYSRYFG
                     SVFLNQHRELILNQQWVTDWELTAMQNALLDWGSNPDAFYARCRCEAVGWK"
     gene            complement(2367..2735)
                     /locus_tag="VN039_03610"
     CDS             complement(2367..2735)
                     /locus_tag="VN039_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45105.1"
                     /translation="MTMVLLAASLAGCATGRYTPLGDAEKTEIQQLKGNVYRVEYRVS
                     AFTSQEQLDRYLRQRCAELTLREGYDFFHLARRADMLGLSRRTAMTVTLYKGQKPVGV
                     ADLYDAKDVLAEAATVLKSY"
     gene            complement(3002..3460)
                     /locus_tag="VN039_03615"
     CDS             complement(3002..3460)
                     /locus_tag="VN039_03615"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWV45106.1"
                     /translation="MWDKKRGDMESENGNFTVLGKDVTFKGIVHFHSTVQLDSAFEGE
                     IHAKGMLVIGENAVVRGSIIAETIVSRGKIHGNVSASGKIQLLKSAVLLGDVSSPSFS
                     MEEGAFFKGSIDMGSHPVVDELQQSTMVLPDFSHRLSPSRPVLIESERGG"
     gene            3771..4208
                     /locus_tag="VN039_03620"
     CDS             3771..4208
                     /locus_tag="VN039_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HWV45107.1"
                     /translation="MIRPSTTGMYREPVTMDLDTSSRWLVLVGCICAGLGVAAGAFGA
                     HMLKDLLDQPMLAVYDTATRYQMYHAFGMVLSGFAVRIGRDAGAAKAGWLFLAGILLF
                     SGSLYGVSLLGVRWLGAVTPIGGALFIIGWCVLAWRAWRWTAS"
     gene            complement(4209..5105)
                     /locus_tag="VN039_03625"
     CDS             complement(4209..5105)
                     /locus_tag="VN039_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45108.1"
                     /translation="MAGFLLIVIFSMLARSIAMAEGTVSPILLAEVAGQQADQLASIA
                     SDEAALTLFIKTVGPALGLKDAAGTLGAKRLPGKMVKELGLAELSNSVYELMAALSAW
                     QFADSLGHDVGQSSSGTPLPASARQDWLTNRSRVDSLPDLFRLSQEAQTLRTSATTPM
                     QQNSDLPLAALRTVFDSSQQATAAWWEIYGWKERVRQAKGRARLCGTWQWVIHNHQHH
                     EEQKHTLVFPPDGPMSATIPALSEMIMLGDGIYLRWEHNGQIQEDSLLFTKDNTKLEG
                     SFVNSMGGWGAISGKRTAPCRP"
     gene            complement(5204..>5943)
                     /locus_tag="VN039_03630"
     CDS             complement(5204..>5943)
                     /locus_tag="VN039_03630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWV45109.1"
                     /translation="PSGIPIPTHTDQMVDPYFKSKIPKEHHIDCLLFWPQQAGAFPGL
                     VLLHDWWGLTGQMKDLGARLACEGYTVIIPNLYGRLGGMVTANDDVAAALLERQNDAQ
                     VITDINSCCEYLNTRLFVKKNIHGVVGYGMGGSYALRFACHRKRLRAAVSYYGKMVTP
                     KELMKDLFSPVLYHQAGKDTWATQADAEQLRSASTDYAKRVEIHLYPEASHAFCNDMK
                     PHAYDPDSSALAWERTATFLKSCFQGL"
BASE COUNT         1244 a         1598 c         1774 g         1327 t
ORIGIN      
        1 caacaccgga ggcttgtcgg tgccgtcatg caggcagaac accgaatggg tcatgccgcc
       61 atgggcgaat ggttggtcga aacactcgac ccaatttcga gtgggcgtac cttcttcttt
      121 cgtatgcggc gcacacatcc caagctgtgt tgctccacag cctgcctgaa ataagagtag
      181 tgccacgatg atggtgtgcg agagagagcg gcacagtgta ttcatattgt gttccggcgc
      241 aatgtgctgc ggctcgtatt atgcctgctg tgaggtaaat cagcggaaga ttgccagcgt
      301 cagaggatcg gcgcaagacc ttgtatgtcc ttacgaacat ttcgcgatat gtccattgtg
      361 agggagtgtg gtaccatccg tcgctgtgat aacagaaaca gaaggtcccc tctcaattcc
      421 gtcgacgccg gaatctatta gccctacctg gctcacgacg acgctgcgtc gtgccggcgt
      481 gctcgaccga gccgcagttg tggggatcac tgtgacaccg gtggccgggg gtagtggatt
      541 cgtagggcaa acagcccgtc tccgagtcgc gtacgatgcc cgtgaagccg gcgctcccgc
      601 caccgtcttt gccaaattgt cctcagcgga tcctgccgtc aggcagcaac tgcggagggt
      661 ggggatctat gaaaccgagg ctggtttcta ccgagacatg gctctgcagc cgaccttccc
      721 tatacgcgtg ccccgtccgt acctgagtca gtatgatgaa gacacaagcg caagtattct
      781 attgctggaa gatctcagcg cggtggaatt tggcgacaat ctcactggtt gttcagcaac
      841 ggacgctcgg atcgccgtgc gacagctcgg tctcttgcac gcacactgtt ggaacgcccc
      901 tctccttaaa tccttgtcat ggttgcgcgc gctcactgat gaggtccagg caagagccgg
      961 catgtaccag gcgatgttgc ctcgcttcga acaacggtgc gctgagtttc tgacacccgc
     1021 tttacgtgag gctgcacgaa gattcgcatt ggtgttccct gaatattctc aacagtgctc
     1081 aaatggtcct cagacactca cgcacggcga cttccgagcc gacaatttgg catttgtcca
     1141 gacaagcgag ggacgaggcg tgaccgtatt tgattggcag gtggtcaggc gctcccgtgg
     1201 cccgcgcgac ctggcgtatt ttctgtcaag ctcgctgtcg gtggagcagc gacggactct
     1261 ggaggagtcg ctgctggatt tgtacgatga gacgctgaaa gccaacggcg tggaagggta
     1321 ttcgaccgga gacctccgtc gcgacattca agccgggctc ggtgcgccgc tgactacatg
     1381 ggtgattgcc ggtggcatgc tcgacttctc cagtgaacgc gggaccgact tgttgaaaca
     1441 ggtgtgtgcg cggcttggcg cggcgctgga cgatcatcgg tttatgagct atctcgacga
     1501 acttgcctga cgccgcactg gttcagtcag caacatggct ctgaagcaac acgaaatcta
     1561 ttccgcaggc tacgatccgc tggtgttgca aggactggcg agacgaagtg cgactcgcga
     1621 cgccgcgttc tttgtgcccc atctcacacc tgtcatgcac gtgttggact gtggatgcgg
     1681 tccaggaggg attagtgtca ctcttgcggc attggtccca cagggacgag tcgtgggggt
     1741 tgatattgag gaccgccagc tcgacatggg ccgccgagag gcgcagcagc gtggcatcgg
     1801 caacgtcgag ttccaccatg ccagtctgta cgctctaccg tttgcggacg agtccttcga
     1861 cgcggtcttg gcccacgccg tcctctatca tctcgccgag ccgatgaacg ccttgcggga
     1921 actgtggcgt atcctcaagc cgggaggtgt gattgggttg cgcgatgcgg atttcgatgg
     1981 tgatgtctat tacccggccc atgaagacgt cgatcggttt tggaagctga cgatgcgggt
     2041 gattgaacac aatggcggcg atcctcggtt cgggcgaaaa cagcgccggt tgttacggga
     2101 agcgggattt cgcaacattg caggctcggc gtcgtccgat gccttcggga ccctggagat
     2161 gaccgctggc tacagccgtt acttcggcag tgtctttttg aatcagcacc gcgagctgat
     2221 tctgaaccaa caatgggtaa cagactggga actcaccgct atgcagaacg cgctactgga
     2281 ctgggggagc aaccctgatg ctttctatgc ccgctgccgc tgtgaagcag tggggtggaa
     2341 gtgaatctgc cctcgcgatg gtctccctag taagacttca gaacagttgc ggcctcggca
     2401 agcacgtcct tggcatcgta gaggtccgca accccgacgg gcttttggcc tttgtacagc
     2461 gtcaccgtca tggccgtccg acgtgagagc cccagcatat cggcgcgccg agccaggtga
     2521 aaaaagtcat agccctcacg gagggtcagc tccgcacagc gttggcgaag atagcggtcc
     2581 agctgctcct gcgaggtgaa agcgctcacg cgatattcca cccgatagac gtttcctttg
     2641 agctgctgga tctctgtctt ttctgcatca cccaacggcg tatagcgccc ggtcgcacaa
     2701 ccggcaagag acgcggccag cagtaccatc gtcacttctg tcttccaaag cttgggccac
     2761 atagaaggtg gccatcgtag cggcacgggc caacgagatc aacaacaaac cccctcctac
     2821 ctcttggggt ggaccagcct cgggacatgt cgctcattcg tgcaatgcac aacacaagat
     2881 ccgatcccca atcaacgact acgaggcgca gagggaatgc agatcaatgg aaaaggttgc
     2941 ttaaaacttt cgagaagaag aagcgtttga gcagttcaga aggaaccgat ccgagggaac
     3001 gtcagccccc tcgttcactt tcaattagaa ccgggcgcga tggactgagc cggtgcgaaa
     3061 aatcgggcag gaccatggtc gattgttgca gttcatcgac caccggatgg gagcccatgt
     3121 cgatcgaacc cttgaagaac gcgccttctt ccattgaaaa cgacggagag gagacatccc
     3181 cgagcaggac cgcggacttg agcagctgga tcttgccgct ggcgctcacg ttgccatgga
     3241 tttttcctct gctcacaatc gtctcggcga taatcgaacc tcggacgacc gcattttcgc
     3301 cgatgaccaa catgcctttg gcatggatct ctccttcaaa ggcactatcg agttgaacgg
     3361 tgctgtgaaa atggacgatg cctttgaatg tgacgtcttt ccctaaaacg gtaaagttcc
     3421 cgttctctga ctccatatcg cctcgctttt tgtcccacat ggatggtccc tttcggagtg
     3481 gtttcgaaaa aatagagatc caccatacag gagtcggaag caagaatcaa aggctgattt
     3541 tgggatgtgc ttgatgagag aagaaaacta cttcttggta gcgggaggat cctgcattcg
     3601 tgactttgcc gaactatttc ctgcgataga tgtgacggtc ttcagaagga aattgaggac
     3661 gccggatatg cagctagttg cccatcgtcg ggccataggg gaaactagac cataacgagg
     3721 aaagttcctc gacaggggac agtggatgga gtattcagcg gggatcgcgt atgatccgac
     3781 cgtctacaac cggcatgtac agggagccag tgacgatgga tctcgatacg tcatctcgat
     3841 ggttggtgct cgtgggatgt atttgcgccg gactcggcgt ggctgccggc gcattcggag
     3901 cccatatgct caaagatctg ctggatcagc cgatgctggc tgtctacgat actgcgaccc
     3961 gctatcagat gtatcacgcc ttcggtatgg tcttgagcgg gtttgccgtt cgcatcggtc
     4021 gcgatgccgg agcggccaaa gccggatggc tgttcttggc aggaatcctc ttattctcag
     4081 gtagtctgta cggagtatcg ttgctgggag tgcgctggct cggagcggtg acgccgatcg
     4141 gaggcgcatt attcattatc ggctggtgcg tgttggcttg gcgcgcctgg cgttggacag
     4201 caagctgact acggtcggca gggggctgta cgctttccgc tgattgcccc ccatcccccc
     4261 atgctgttta caaatgatcc ttcgagcttc gtattgtcct tggtgaacag cagactgtct
     4321 tcttggatct gcccgttgtg ttcccatcgc aaataaatgc cgtcgccgag catgatcatt
     4381 tccgacaagg cgggaatagt ggccgacatc ggtccgtcag gaggaaagac cagggtatgt
     4441 ttctgctctt cgtggtgctg atggttatga atgacccatt gccatgttcc gcagagacga
     4501 gcccggcctt tcgcttgcct gacacgttct ttccagccat agatctccca ccacgctgca
     4561 gtggcctgct ggctggagtc aaatactgta cgcagagcgg ccaacggaag atcgctgttc
     4621 tgctgcatgg gagttgttgc agatgtccga agggtctggg cttcttggct gaggcggaaa
     4681 aggtcgggga gcgagtcgac ccggcttcgg ttcgtcagcc aatcctgccg tgctgaagca
     4741 ggtaatggag tgccggaaga agactgaccg acatcatgac cgagtgaatc agcaaattgc
     4801 caggcggaca acgccgccat caactcatag acggagttgg acagttcagc caatcccagt
     4861 tctttgacca tcttgccggg gagtcgtttg gctcccaatg tccctgccgc atctttcaaa
     4921 ccaagggccg ggcccacggt cttgatgaac agtgtcaggg ctgcttcatc ggaggcaatc
     4981 gaagcgagct ggtcagcctg ttgacccgcc acttcggcca acaggatcgg agacaccgtt
     5041 ccctctgcca tggcaatcga ccgggcgagc atcgagaata ttacgatcag gagaaagccg
     5101 gccatggtat ggcgatggac gtggtgagct tggttccggc cgagagttcg tgcgatcgtg
     5161 accggtgtgc ggggttgaga gtgtcttggg gagcggcgcg gggtcatagt ccttggaagc
     5221 aagacttcaa gaaggttgcg gttcgctccc aagcaagggc cgaactgtcg ggatcgtacg
     5281 catgcggctt catgtcatta cagaaagcgt gggacgcctc ggggtagaga tgaatttcga
     5341 ctcgcttggc atagtcggtg gaggcgctgc gcaattgctc ggcgtcggcc tgagtggccc
     5401 aggtatcctt tcctgcctgg tgatacagga caggcgaaaa gaggtctttc atcagctctt
     5461 tgggagtgac cattttaccg taataggaaa cggcggcgcg caatcgcttc cgatgacagg
     5521 cgaagcgcag cgcataggac ccacccattc cataaccgac gacgccatga atgttcttct
     5581 tcacgaagag gcgggtattg agatattcac aacaggagtt gatatccgta atgacttggg
     5641 catcgttctg acgttccagc agagcggccg ctacgtcatc gttggcggtc accattcctc
     5701 ccaaccggcc gtagagattc ggaatgatca ccgtatagcc ttcacaggcc agtcgggcgc
     5761 cgagatcttt catttgtccg gtcagacccc accaatcatg caataggaca agacccggaa
     5821 aggcgccggc ttgttgcggc caaaagagca ggcaatcgat gtggtgttcc ttcggtatct
     5881 tgctcttgaa gtaggggtcc accatctgat cggtgtgggt gggaatcggt atgccgcttg
     5941 gga
//
LOCUS       DATMTI010000214         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_518191, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000214 DATMTI010000000
VERSION     DATMTI010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518191"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VN039_03635"
     CDS             <1..1506
                     /locus_tag="VN039_03635"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWV45110.1"
                     /translation="PVVGAVVLWLFDEEDMVRTSALTIALVELALSVFVLLRFVPESA
                     AMQFTERVRWIPALGISYHLAVDGISVLFVGLTAFLTVLVVIYSWDTIRHQMKLYMMC
                     LLALETTTMGVFVSLDLILFFVFWELMLIPSYFLIKLWGGGAERHYAALKFVIYTLLG
                     SVFMLVGIALLNINYHQWASLHHTDQVYSFDLLELLTVPIPISQQILIFWLMFMGFAF
                     KAPVFPFHTWLPDALLEGPIGMAVVLAGLKLGTFGFIRFSIPLLPDASKSETVVMVVV
                     VLGLCAILYGAWMALVQADFRRLLAYSSVSHLGFVIVGLFALNYQGLQGSLLTMINLG
                     FSTAGLFFIAGFLYSRQQTTQLSAFGGMAKQVPLLATFFLIIGLASIGLPGTNGFVGE
                     FLILLGAFKAKWWFGAIAVLGVIFGAAYFLWYYERSMLGPLGAAVKGTMSDLQPREKI
                     IALSLSIMILWIGLYPSPFLRIMNGSVQALVDRVSQETKVSLEGARFGREY"
BASE COUNT          290 a          412 c          480 g          498 t
ORIGIN      
        1 ccggtcgtcg gagcggttgt gctgtggttg ttcgacgaag aagatatggt ccggacctca
       61 gcgctcacca ttgcgctggt tgagctggca ctttctgtgt ttgtcctgct gcgtttcgtg
      121 ccggaatcgg ccgccatgca attcacagag cgtgtccgct ggattccggc gttaggcatc
      181 agctatcacc tggcagttga tggaatcagc gtgttgttcg ttgggctcac ggcttttctg
      241 acggtcttgg tggtgatcta ctcctgggat acgatccgac atcaaatgaa actctacatg
      301 atgtgtctat tggcgctcga aacgacgacg atgggcgtct tcgtatctct ggatttgatc
      361 ctgttcttcg tgttctggga gctgatgctg attcccagct acttcttgat caagttgtgg
      421 gggggaggag ccgagcgtca ctatgccgcg ctgaagttcg tcatttatac gcttttaggc
      481 agtgtgttca tgctggttgg catcgccttg ctgaacatca attaccatca gtgggcatcc
      541 cttcatcaca ctgatcaggt ctattcgttc gatttgctag aactgctgac ggtgccgatt
      601 cccatcagtc aacaaatcct tatcttctgg ctgatgttca tgggatttgc gttcaaggcg
      661 ccggtgtttc ctttccatac atggctgccg gatgcgctgc tggaagggcc gatcgggatg
      721 gctgtggtgt tggctggtct caagctcggt acattcggct tcatacgctt cagcattccc
      781 ctgctccccg atgcgtcgaa aagcgagacg gttgtcatgg tggtggtcgt gctcgggctc
      841 tgcgcaattc tctatggggc ttggatggct ttggttcaag ccgattttcg acgattgctg
      901 gcctatagta gtgtcagtca tcttggtttc gtcatcgtag ggttgtttgc attgaattat
      961 caaggtttgc aaggcagctt gctcacgatg atcaatctgg ggttcagcac ggcaggactc
     1021 ttctttatcg cggggtttct ttactcgcgc cagcagacga ctcagttgtc ggcgttcgga
     1081 gggatggcga aacaagtgcc tcttctagcg accttttttc tgattatcgg actcgcgtcg
     1141 ataggacttc ccggtaccaa tggctttgtc ggcgagttcc tcattctttt gggcgcgttc
     1201 aaagccaaat ggtggttcgg ggccatcgcc gttctgggag tgattttcgg cgccgcgtat
     1261 tttctctggt actacgaacg ttcgatgtta ggtccgttgg gtgctgccgt gaaagggacg
     1321 atgagcgatc tgcaaccgcg cgagaagatc attgcgctgt cgctgtcaat catgattctc
     1381 tggatcgggc tctatccctc accgttcttg cgaatcatga acggatcggt ccaagcgctc
     1441 gttgatcgag tctctcagga gaccaaggtg tcattagaag gcgcccgatt cgggagagag
     1501 tattaggtcg cgcgatggga gagtacgcac tgctctatat cctgtttgcc ccgtttgccg
     1561 gggctctggg gttgattttt atttccaatc gacagcctct gctcgttcga agtatcgcag
     1621 caagtgccgc atttgtgtcg ctgatctcgt cgttctatct cttttacgcc tatgatccag
//
LOCUS       DATMTI010000215         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_645616, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000215 DATMTI010000000
VERSION     DATMTI010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645616"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..2716
                     /locus_tag="VN039_03640"
     CDS             65..2716
                     /locus_tag="VN039_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HWV45111.1"
                     /translation="MMQRLVPVMGLIFLTLAVQPAVAQAGATTAPYLNTNLTAEQRAT
                     DLVHRMTLAEKASEMQNNSAAVPRLKVPAYQWWSEALHGVINKGVTEYPEPIGLAATF
                     DPLGIHTMAGQIGIEGRIKHVQNLREGHTGIMGGLDFWAPNLNIFRDPRWGRGQETYG
                     EDPFLTGRMGVAYIKGLQGDNPKYYLAIATPKHFAVHSGPEPTRHFADVDVSKHDEVD
                     TYEPAFRAAITEGKAGSIMCAYNAINGEPACANQYLLQDQLRGKWGFKGYVVSDCDAV
                     RDIAANHRYRPTQAQGVAISVIRGMDNECVTFTSRFGEPVDKAYIDAVQQGYLPESTL
                     DTALIRLFTARIKLGMFDPPSMVPYTKIDEKELDSAEHRAQALKLANESMVLLKNDGL
                     LPLKAGIKKIAVIGPLADQTTPLLGNYAGQPTHIVSILDGIKAEFPNAKITYVSGTQF
                     LRNDGEPVPDALLTTPDGKPGLKAEYSESRNGPGAQSNPTLATRIERNANLSKSDVPS
                     EVAGKKTIAVHWTGSITPTETGDYLLGVRGTGFGRLTIDGKQVAVLVAYDEAEAATGR
                     VHLVKGQKAELSLEYGSRPNAPRAQLIWAKVNDAPSPEAIAAAKNADAVIAVVGITSR
                     LEGEEMPVSIPGFLGGDRTSIDLPQPEEALVEAVAATGKPLAVVLLNGSALAVNWINK
                     HANAVLEAWYPGEEGGAAVAETLSGKNNPAGRLPVTFYTGVDQLPNFEDYGMANRTYR
                     YFDGKPLYPFGYGLSYTTFRYSDLSVPSQAVAAGEPVGADVTVTNSGKVAGDEVFQLY
                     LKFPDVKGAPLLALRGFQRVHLEPGASQKVHFELKNRDLGVVTEEGNPIIAQGDYTIS
                     IGGGQPNTGAQVVTGKFHIEGQIDLPE"
BASE COUNT          688 a          806 c          829 g          541 t
ORIGIN      
        1 gctgagagac agccgccgaa gtcacgaacg tgaggcaaaa acaatgatga agtggatgac
       61 ccaaatgatg caacgtcttg tcccggtaat gggactcatt ttcctgacgc tggctgtaca
      121 gccagcagtc gcgcaagctg gtgcaacgac agctccgtat ttaaacacca atttgacggc
      181 agagcagagg gctaccgacc tggtgcaccg gatgactctc gccgagaagg cctcggagat
      241 gcaaaataac tccgccgcag tgccgaggct caaggttccc gcatatcaat ggtggagtga
      301 ggccctccac ggggtcatca acaagggagt gactgagtat ccggagccaa tcggcctggc
      361 tgcgaccttc gacccccttg gaattcatac catggcgggc cagatcggta ttgaggggcg
      421 catcaagcat gtgcagaacc tgcgcgaagg acacactggc atcatgggag gcctcgactt
      481 ctgggccccg aatctgaata tcttccggga tccgcgctgg gggcgcggac aggagaccta
      541 cggcgaggac ccgttcctaa ccggacgtat gggggtggcc tacattaagg gcttgcaggg
      601 agacaatccg aagtactatc tggccatcgc aacgccgaag cactttgcag tgcacagcgg
      661 accagagccc acgcggcact ttgccgacgt tgacgtcagc aaacatgatg aggttgatac
      721 ctacgagcca gcattccgtg cggcaattac ggaaggcaag gccggttcga tcatgtgcgc
      781 atacaacgcc attaatggcg aaccagcgtg cgccaaccaa tatctgttgc aggaccaatt
      841 acgcggcaaa tggggcttca agggatacgt ggtctcggat tgcgacgccg tcagagacat
      901 cgcggccaat catcgctatc gtccaacaca ggcccagggc gtggccatct cggtgatccg
      961 cggcatggac aacgagtgtg tcaccttcac ctcgaggttt ggcgagccgg ttgataaggc
     1021 ctatatcgat gcagtgcagc aagggtactt gccggaaagc acgttggaca cagcgctgat
     1081 ccgtctgttc accgcgcgca tcaagcttgg catgttcgat cctccgagca tggttcctta
     1141 cacaaagatc gatgagaaag aactggatag cgcagagcac cgcgcccagg ctctgaagct
     1201 ggctaacgag tcgatggtcc tcctgaagaa cgatggtttg cttcctctaa aagcgggaat
     1261 caagaagatc gccgtgatcg gtccgctggc ggatcagacc acgccactgc ttggaaacta
     1321 cgccggtcag cccactcata tcgtctccat cctggacggc attaaagcgg agttcccgaa
     1381 cgcgaagatt acctacgttt cgggaaccca gttcctgcgc aacgacggag agcccgttcc
     1441 ggatgccttg ctgacaactc ccgacggcaa gcctggcctc aaggcggagt acagcgagtc
     1501 gagaaacgga cccggcgccc agtccaatcc aaccctcgcg accaggattg agcgaaatgc
     1561 caacctctcc aaaagtgatg tgccatcgga ggttgccggc aaaaagacca tcgccgttca
     1621 ttggacaggc tcaatcactc cgacggaaac cggcgattat cttctcggcg tgcgcggaac
     1681 aggcttcggg agacttacga ttgacggcaa gcaggttgca gtgctggttg cctacgacga
     1741 agcggaagcc gcgactggtc gcgtacacct tgtgaagggg caaaaggccg agctgagctt
     1801 ggagtacggc agccgaccca atgcgccgcg ggcccaactc atctgggcaa aagtgaacga
     1861 tgctccttcg cccgaggcaa tcgcagcagc gaaaaatgcc gatgccgtaa ttgcggttgt
     1921 cggtatcacc agcaggctcg aaggggaaga gatgccggtg agcatccctg gatttttagg
     1981 aggcgaccgc accagtatcg atctccctca accggaagaa gcacttgtcg aagcggttgc
     2041 tgcgaccggc aagccattgg ccgtggttct gctgaacggc agcgccctgg ctgtgaactg
     2101 gatcaacaag cacgccaacg ccgttctcga agcatggtat ccgggcgaag agggcggagc
     2161 agccgtagct gagactctga gcggcaaaaa caatcctgca ggaaggctac cagtcacgtt
     2221 ctataccggc gtcgaccagt tgcccaactt tgaagattat gggatggcca accggaccta
     2281 tcgctacttc gatggcaagc cgctgtatcc attcggatat gggcttagct acacaacatt
     2341 tcgctatagc gacctcagtg tgccttcaca agctgtggct gctggagagc ccgtgggcgc
     2401 cgatgtgacg gtaacaaatt ccggtaaggt cgcaggagat gaggtcttcc agctttatct
     2461 caaattcccc gatgtaaagg gtgcgcctct cctcgctctc cgcggattcc aacgcgttca
     2521 tctcgaaccg ggtgcaagcc agaaagtcca tttcgaattg aagaaccgcg atctgggagt
     2581 tgtgaccgaa gagggaaatc ccattattgc tcaaggggat tacacaatca gcatcggtgg
     2641 aggtcagccc aacaccggcg cccaagtggt tacgggaaag ttccatatcg aaggccaaat
     2701 cgacttaccg gaatagggca tccactcagc gtccccgctc ccttcctcga ataagggagc
     2761 gggaacgttt acagacacca tttgctgtct tgaattgacc gccgactcga cgcccgatta
     2821 tggaatgcgt catcgggcgg tacaacggat ctcaacatgg tcgg
//
LOCUS       DATMTI010000216         6011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_348419, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000216 DATMTI010000000
VERSION     DATMTI010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348419"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..299)
                     /gene="acpP"
                     /locus_tag="VN039_03645"
     CDS             complement(66..299)
                     /gene="acpP"
                     /locus_tag="VN039_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWV45112.1"
                     /translation="MATVEERVKKIIAEQLGVEEDEVTPEASFVEDLGADSLDTVELV
                     MALEEEFSIEIPDEDAEKILTVGKALDYIKEKS"
     gene            complement(384..1127)
                     /gene="fabG"
                     /locus_tag="VN039_03650"
     CDS             complement(384..1127)
                     /gene="fabG"
                     /locus_tag="VN039_03650"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HWV45113.1"
                     /translation="MSLQGKTAIVTGAAQGIGRAIAECFAQAGADIAVADLDPGRSVE
                     TVASVEKLGRKALNIKVNVADANETKAMVEQVLKVWGKVDILVNNAGITRDGLLLRMK
                     EEDWNLVLQINLNGTFNCTKAVLQPMTKQRYGRIVNIASIVGIIGNAGQANYSASKAA
                     VIGFTKTVGREYASRNVTVNAVAPGFIDTAMTHGLPADVKDTLLKQIPLGRLGTPADV
                     AAAVRFLVSEDAAYITGHVLHVNGGMLMV"
     gene            complement(1204..2139)
                     /gene="fabD"
                     /locus_tag="VN039_03655"
     CDS             complement(1204..2139)
                     /gene="fabD"
                     /locus_tag="VN039_03655"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWV45114.1"
                     /translation="MTLGIGLVFPGQGSQSVGMGKALYDAHPLLKPVYDEASSVLGYD
                     IAELCFTGPAERLNRTEFTQPALLVSSVAALKLFEPAGIKPVAVAGHSLGEYSALVAA
                     GGVSFHDAVGLVQKRGRYMSEAVAPGAGLVAALLGLTAEVVKDVCRTASSVGVVAAAN
                     FNSPGQVVIAGEKAAVERAIELAKAQGCKKAIPLPVSVPVHTPLMQQAADRLAKDLAT
                     VRWSDLNAPLVNNAEAKAISLAHEIQASLVRQLPSSVLWEDTVQTMGRMGVTTFVEIG
                     PGTVLIGLIRRILPEAKLLNVNDPKSLDATLKAVS"
     gene            complement(2267..3241)
                     /locus_tag="VN039_03660"
     CDS             complement(2267..3241)
                     /locus_tag="VN039_03660"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWV45115.1"
                     /translation="MKAWIAGTGSYTPARVLTNAELERMVATSDEWIRERTGIRERRI
                     AATGEACSDLAVQAGRRALAEAGLAATDLDMILVATCTGDYPLPATACLVQHQLGAAK
                     AAACDLSAACCGFVYALSVADAYVKNGMRHVLVIGSEVMSAITDWTDRNTCVLFGDGA
                     GAVVVSASDGERGILSTHLRSDGALCELIMVPGGGSRTPPSEKVIAEGLQYLKMKGNE
                     TFKVAVRTLEEIARTTLSANGLRVEDLDLYVPHQANVRILKAVMERLGLPIEKVLLNL
                     DRYGNTSAASIPIALDEAVREGRIKDGSLVMLGAFGAGLTWASAVIRW"
     gene            complement(3238..4254)
                     /gene="plsX"
                     /locus_tag="VN039_03665"
     CDS             complement(3238..4254)
                     /gene="plsX"
                     /locus_tag="VN039_03665"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWV45116.1"
                     /translation="MKIALDAVGGDHGPAPCVEGALQAVKELDVEVILVGDETTLKQE
                     CERQSCRDSRLTIQHASQVVEMHESPAAVARKKRDSSIWIATELVKNGHADAVVSPGN
                     TGASMVSSFFVLGLTKGVERPAIATSLPTLSGEAIMLDVGANVDCTATHLEQFALMGN
                     EYGKHLLCKPNPRIGLLSIGEEDGKGNEVTKEAFKLLKGSSMNFVGNIEGRDVYSGIA
                     DIVVCDGFIGNVALKISEGVAEMIKRLLLKEISGHFLGRLAYPLISGPLMNLKRKIDY
                     AEFGGAPLLGVNGITMICHGRSSAKAIKNAIRRAKGMAEGGVRELIQRDIEESRSRPQ
                     VEPT"
     gene            complement(4371..4556)
                     /gene="rpmF"
                     /locus_tag="VN039_03670"
     CDS             complement(4371..4556)
                     /gene="rpmF"
                     /locus_tag="VN039_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWV45117.1"
                     /translation="MPNPKHKHSRARRDKRRTQKLRMTPPGMAVCPQCHELKLPHYTC
                     LNCGTYKGKAVIQVEEA"
     gene            complement(4592..5242)
                     /locus_tag="VN039_03675"
     CDS             complement(4592..5242)
                     /locus_tag="VN039_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWV45118.1"
                     /translation="MIDDFDAPRWNSGTLPRMTMDVLTPKIADITAEGLSLSGDLTGE
                     ELGLTDTDVSIHGTLAVGLDLRAIERTIYVTGVVEGTAVRQCVRCLREFDDPLAFSLR
                     VAYEREVKAMGAVTKRDDPRKRKPAAPPDGEVEEQNDDLYYFAGDHLELAPMLREQLI
                     LASPMHPLCSEECRGLCARCGKDLNEGPCRCGEEPTGSPFQVLRTMKDKLKDPGAR"
     gene            complement(5248..5496)
                     /gene="rpsR"
                     /locus_tag="VN039_03680"
     CDS             complement(5248..5496)
                     /gene="rpsR"
                     /locus_tag="VN039_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWV45119.1"
                     /translation="MDRSDNERGGGRLFQRRRPCRFCLEKAPIDFKDAGLLRNFLTER
                     GRIVPRRISGNCMRHQRELTVAVKRARHIAIISFAEER"
     gene            complement(5524..5922)
                     /locus_tag="VN039_03685"
     CDS             complement(5524..5922)
                     /locus_tag="VN039_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWV45120.1"
                     /translation="MAGFNKVILMGNLTRNPELRYTPSGTPVASFGLAVSRRFKQGDD
                     LKEEVCFVDIVVFGRQAEHCGQYLSKGNGAIVEGRLQQRRWETEDGQKRSKHEVVAQT
                     VTFMPKRQEGGAGAEPPMHDDPGYDMGEEG"
BASE COUNT         1253 a         1928 c         1492 g         1338 t
ORIGIN      
        1 caccagccca agaccggtga ccacaacgcg ccgtgccgct tgatccgata ttccggtcgg
       61 catgcctagg acttttcctt gatgtagtcc aacgccttcc ccacggtcag aatcttctcg
      121 gcatcctcat cgggaatttc gatcgagaac tcttcttcca acgccatgac aagctcgacg
      181 gtatcgagcg aatcagcccc aagatcttcg acgaaggagg cctccggcgt cacctcatcc
      241 tcttctacac cgagctgctc agcaataatc ttcttgactc gctcttcaac agttgccatc
      301 gctatgacta cctccctccc cgatatgact accactgcgc cgtcacatgg ggatctgtga
      361 cggtcgtaca aacaattcga cttttatacc atcaacattc cgccgttcac gtgcaagaca
      421 tgaccggtga tgtaggccgc atcctcggac accagaaacc gcacggccgc cgcaacatcc
      481 gccggtgtac ccaaccgtcc caatgggatt tgtttcagta acgtgtcttt cacatcagcc
      541 ggtagtccat gggtcatggc cgtgtcgatg aaaccgggcg ccaccgcatt caccgtgacg
      601 ttgcggctgg catattcccg cccaaccgtt tttgtaaacc cgatgacggc tgccttcgag
      661 gccgagtaat ttgcctgccc cgcgtttccg attatcccga cgatcgaggc gatgttgacg
      721 atgcgaccat accgttgctt ggtcatcggc tgcaagaccg ctttcgtgca gttaaacgtg
      781 ccgttcaggt tgatctgaag caccagattc caatcttctt ccttcatccg tagcaacaag
      841 ccgtcacgag tgatgccggc attattgaca aggatgtcca ctttccccca gaccttcagg
      901 acttgctcga ccatcgcctt ggtctcattg gcatctgcga cgttgacttt tatgttcaga
      961 gccttccgtc ccagcttttc gacggaggcg accgtttcca cggagcggcc ggggtcgaga
     1021 tcggccacgg caatgtcggc tcccgcttga gcaaagcatt cggcaatggc ccgaccgatg
     1081 ccttgcgccg cgccggtgac aatagctgtt tttccttgta gtgacatttc agacctttcg
     1141 gacgatacgt taacaggctg acagggaatt cgtcacctgg ttttctatgt ggacggtccg
     1201 gccttagctg accgccttga gtgtcgcgtc caacgacttc ggatcattca cattcaagag
     1261 tttcgcctca ggtagaattc gtcggatcaa gccgatcagt accgtaccag gcccgatttc
     1321 tacaaaggtc gtgacgccca tccttcccat tgtctgcacg gtatcctccc acagtactga
     1381 agaaggcagc tgacgaacca acgacgcttg gatctcgtgc gccaggctga tcgctttcgc
     1441 ctcggcatta ttcacaagag gggcgttgag atccgaccat cgaaccgtgg ctaaatcctt
     1501 cgccaatcga tcggctgctt gctgcatcaa cggggtatga accggcacgc tgaccggcag
     1561 tggaatggcc tttttacacc cttgcgcttt ggccaattca atcgcccgct ccaccgccgc
     1621 cttctcaccg gcaatgacca cttgcccagg cgagttgaag tttgccgccg cgaccactcc
     1681 aacggacgac gccgtgcggc aaacatcctt gaccacctcg gcggttaaac ccaacagagc
     1741 ggcgaccagt ccggctcccg gagccacggc ttcggacatg tagcgtcccc gtttctgaac
     1801 caggccgacg gcatcatgaa aggatacgcc gcccgccgca accagcgcag aatattcacc
     1861 taaactatgg cccgccaccg caaccggctt gatcccggcc ggctcaaaca gtttcaacgc
     1921 cgccacactg ctcacaagca aggccggctg agtgaattct gttcgattga gtcgttcagc
     1981 cggtccggtg aagcacaatt cggcgatatc ataacccagg accgaagatg cctcgtcata
     2041 gacgggtttc aataggggat gcgcgtcata gagcgccttc cccatcccga ccgactggga
     2101 gccctgtcca gggaaaacaa gcccgattcc tagcgtcatg gggcgttaga tatcttcgaa
     2161 atcccgcgca aagtcaacgg gaaaagtttt cactatccgc cgtcttccta caaccaacta
     2221 aagggctgcg aatcagaatg attctggatc acgacccggt cgttctctac catcggatga
     2281 ctgcagaggc ccaggtcaat cctgccccaa acgcccccag catcaccaac gagccatcct
     2341 tgatacgccc ttcgcgaacc gcttcatcca gagcaatcgg gatggatgcg gcggacgtgt
     2401 tcccgtaacg atcgaggttc aggagcacct tttcgatcgg aaggcccagc cgctccatca
     2461 ccgccttcag aattcgtaca ttggcttggt gcggcacata cagatcaaga tcctccaccc
     2521 ggaggccatt ggccgaaagg gtcgtgcgag cgatttcttc taaagtgcgt accgcaactt
     2581 tgaacgtctc attccctttc atcttgaggt actgcaaacc ttcggcgatc accttttcgg
     2641 acggtggagt acgtgagcct cctccaggca ccatgatcag ctcgcagagc gctccgtcgg
     2701 agcgaaggtg agtcgagagg atccctcgtt ctccatcgct tgcactgaca acgaccgcac
     2761 cagccccatc tccaaacagc acgcatgtat tccggtcggt ccaatcggta atggcagaca
     2821 tcacttctga tccaatcacc agaacatggc gcattccatt tttaacatag gcatccgcca
     2881 ccgagagcgc gtagacgaac ccacagcagg cggccgaaag atcgcacgcc gcggctttgg
     2941 cggcaccgag ctgatgctgg acaagacagg ccgttgcagg gagaggataa tcacccgtac
     3001 aggtggcgac caaaatcatg tcgagatcgg ttgccgccag cccggcctca gccaatgccc
     3061 gcctcccggc ctgaaccgcc aaatccgaac aggcttcccc tgtggctgcg atacgccgtt
     3121 cgcggatgcc ggtccgttct cgaatccatt catcggaggt ggcgaccatc cgttccagct
     3181 ccgcattcgt caggaccctg gcaggcgtat aggagccggt tccggcaatc caagctttca
     3241 tgtcggctcc acctgaggac gggagcggct ctcttcaata tctcgctgaa tcagctctcg
     3301 cacgcccccc tcggccatgc ctttcgctcg tcgaatcgca ttcttgatag ccttggccga
     3361 cgatcggcca tggcagatca tcgtaatccc attgaccccc agcaatgggg ccccaccgaa
     3421 ttcagcatag tcgattttcc gcttcagatt catgagaggc ccggagatta agggataggc
     3481 cagtcgccca aggaagtggc cggagatttc cttgagcagc agcctcttga tcatctcagc
     3541 cacaccttcg gaaatcttca aggccacgtt gccgataaac ccgtcgcaga cgacgatgtc
     3601 ggcgattccg ctgtacacgt cacgtccctc gatattcccc acgaaattca tcgaactgcc
     3661 tttgagcagc ttaaacgcct ctttggtgac ttcgttgccc ttgccgtctt cctccccgat
     3721 gctcagaaga ccgattcggg ggttaggctt acagagcaga tgcttcccat actcgttacc
     3781 catcaaggcg aattgttcga gatgggtggc ggtgcagtcg acgttggccc ccacatcgag
     3841 catgatcgct tccccgctca acgtgggcag gctggtggca atcgccggtc gctcgacccc
     3901 cttcgtcagt cctaacacga agaacgacga caccatgctc gctcccgtgt taccgggact
     3961 cacgacggcg tcggcatggc cgttcttgac caactctgtc gcaatccaaa tcgacgaatc
     4021 ccgcttcttc cgagcgacag cggcaggcga ctcatgcatt tcgacgacct gggaagcatg
     4081 ctgaatcgtg aggcgggaat cgcgacagga ctgacgctca cattcctgtt tcagggtggt
     4141 ttcatcaccg acgagaatga cctcgacgtc caattctttc acggcctgaa gagcgccctc
     4201 gacacagggt gcggggccat ggtcgccccc caccgcgtcg agcgcgatct tcatgcgaga
     4261 tggtgtactc acggatggtg ttggaatgac cggctgaaca cgcgtcgccc cctgggctca
     4321 cgtcagacgt gctgggaaac tcgcgcaatg gaccgacttg tacaacctac tcacgcctct
     4381 tcgacttgaa tgaccgcctt gcccttatag gtcccacagt tcaaacaggt ataatgcggc
     4441 agctttagtt catgacactg tggacacaca gccattcccg gcggggtcat gcgcagcttt
     4501 tgggtacggc gcttatcgcg tctggcccgt gaatgtttat gtttgggatt gggcatgatg
     4561 actccttctt ccgttcgatt cgaaccaccg ctcaacgcgc accggggtcc ttcagcttgt
     4621 ctttcatggt gcgcaacacc tgaaacgggc ttcccgtcgg ctcttcgcca caacgacaag
     4681 ggccttcatt taaatctttt ccacaacggg cgcacaatcc tcggcattcc tcggaacaga
     4741 gcggatgcat gggagaagca agaatcaatt gctccctcag catcggcgcc agctccagat
     4801 ggtcacccgc aaagtaatag agatcgtcgt tctgttcttc cacttctccg tccggaggcg
     4861 ctgcgggctt cctcttcctc ggatcgtctc gcttcgtgac ggcacccatc gctttgactt
     4921 cccgctcata ggccacacgt aatgaaaacg ccagtgggtc gtcaaactct ctgaggcacc
     4981 gtacgcattg gcgaaccgcc gtcccttcca cgactccggt cacatagatg gtacgttcaa
     5041 tcgctcgaag atccagcccg accgccagag ttccgtgaat agacacatcc gtatccgtca
     5101 gtcccaactc ctctcccgtc agatctcccg acaatgaaag accttctgcc gtgatgtctg
     5161 cgattttcgg tgtcaataca tccatagtca ttctcggcaa cgtgccgctg ttccacctcg
     5221 gtgcatcgaa atcgtcgatc acgttcgcta tcgctcctcg gcaaagctga tgatggcgat
     5281 atgccgagcc cgcttgacgg ccaccgtcag ttcacgctga tggcgcatgc aatttcctga
     5341 aatacggcgc ggaacgatcc ttccccgttc cgtcaaaaaa tttcgaagaa gcccggcgtc
     5401 tttaaagtca atcggtgcct tttccaggca gaatctgcag ggacgtcttc gctggaataa
     5461 ccgccccccg ccacgttcgt tgtcgcttcg gtccatgact gcctcctcat aatactcgtg
     5521 ctattaacct tcttcaccca tatcataacc ggggtcgtcg tgcatcggag gttccgcacc
     5581 cgccccgccc tcttggcgct tcggcatgaa cgtcacggtc tgcgcgacca cttcgtgctt
     5641 gctgcgtttt tggccgtcct cagtctccca tcggcgctgc tgcaaccgac cttcgacgat
     5701 cgccccgtta cctttactga gatactgccc gcaatgttcc gcctgtctgc cgaataccac
     5761 gatgtcgacg aagcagactt cttccttcaa atcgtcaccc tgtttaaacc gacggctcac
     5821 cgccaagcca aaactcgcca ccggtgtccc gctcggcgta taccggagct cagggttacg
     5881 ggtgaggttc cccatcagta tgactttgtt aaatccggcc accgtccgac tcctgtgtcg
     5941 aggtttccac cggacgccgc tccatcaagg gcttctcatg atggaccgtc aaaaacttga
     6001 tgatgttgtc t
//
LOCUS       DATMTI010000217         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_560712, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000217 DATMTI010000000
VERSION     DATMTI010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560712"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VN039_03690"
     CDS             complement(<1..429)
                     /locus_tag="VN039_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWV45121.1"
                     /translation="MRITEIFHSVQGESTFAGLPCVFVRLTGCPLRCTWCDTEYAFFG
                     GMDWSIDDILDTVRSYGCRLVEVTGGEPLAQPDTATLLRRLCEEGFTVLLETSGAIDT
                     TKVPPSIRIILDVKCPGSGMTERMHWPNVERLRPQDEAKFV"
     gene            complement(507..1187)
                     /locus_tag="VN039_03695"
     CDS             complement(507..1187)
                     /locus_tag="VN039_03695"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWV45122.1"
                     /translation="MIQTDRRGLSSSVAAFFDVDNTILPGEASELRFFRWLWRRGIVG
                     WPEARESVSWLLRHFPTLSLHPLRERKLYLAGKPSQVIQPLGEEFCREELCPRVSPAA
                     MRMLDEHRRAGHAIVFVTGSIDFLVAPVADALRADRCFASRLEQQNGLYTGFLVPPIP
                     YGEGKRRLIDRLIHELTLDLSQCYAYGDSPGDLDLLRAVGHPTVVNPIRGMASIARRK
                     NWPIVKWR"
     gene            1504..1587
                     /locus_tag="VN039_03700"
     tRNA            1504..1587
                     /locus_tag="VN039_03700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1537..1539,aa:Leu,seq:gag)
BASE COUNT          378 a          435 c          461 g          314 t
ORIGIN      
        1 aacaaatttg gcttcgtctt gcggtcgtag acgttcgaca ttgggccagt gcatccgttc
       61 cgtcatgccg cttcccgggc acttcacgtc caggataata cggatcgacg gggggacttt
      121 ggtggtatcg atcgctccgc ttgtttcaag gaggacggtg aagccttctt cgcacaatcg
      181 acgcagcagc gtagccgtgt ccggttgtgc taagggctca cctccggtca cctcaaccaa
      241 ccgacaacca taggatcgca ccgtgtcgag aatgtcatcg atggaccagt ccattccgcc
      301 gaagaaagca tattccgtat cacaccaggt gcaccgtaga gggcaaccgg tcaagcgtac
      361 aaatacgcag ggcagcccgg caaaggtcga ctcaccttga acgctatgaa aaatttcggt
      421 gatgcgcatg agcagtgtgt ggggttactg agtactcgat tgaatgattg gccacagaga
      481 cggttggtcc gatcacaggc agcagctcat cgccatttga cgatcggcca gtttttacgt
      541 cgggcgatgg aggccatgcc gcgaatcgga ttcaccaccg tcggatgacc tacggcccgg
      601 agcaggtcta aatcaccagg actgtctccg tacgcatagc attgggacag atccagcgtc
      661 agttcatgga tcaaccgatc gatgagtcgg cgtttccctt ctccataagg aatggggggg
      721 acgaggaaac cggtatagag gccgttctgt tgctccaacc gactcgcaaa acatcgatca
      781 gctcggagcg cgtcggcaac gggggcgacc aggaaatcga tggaccccgt cacgaaaacg
      841 atggcatggc cggcacgacg atgctcgtcg agcatccgca tggcggcggg agagacacga
      901 ggacataatt cctcgcgaca aaactcctcg cctaacggct ggatcacttg cgaaggtttg
      961 ccagccagat acagcttccg ctcgcggagc gggtgcagcg acaatgtcgg gaaatgtcgc
     1021 agaagccaag acacactctc tcgagcttca ggccaaccga cgataccgcg ccgccacagc
     1081 cagcgaaaaa atctcaattc gctcgcttcg cccggcaaaa tggtattgtc gacatcaaaa
     1141 aatgccgcaa cggaagagga gaggcctcgt cggtctgttt gtatcatacg aagagtgccc
     1201 taggacaacg aggtgctgat tctaccggac gaccggttga ttgacaagaa ttttacccta
     1261 cttctataat gcgcactcct ttcccctctt agctgacagg aggcctcgat gcaggatggt
     1321 caaacaagcc tgcgacaagg ccgcaacgag gcgcacagtg tcgcgaataa gcgacaccaa
     1381 gtttgtgcgc gccggcgaga tggtgagccg gctgtgtctt gcctgaggcg tactttcaag
     1441 tacggtgaag gtttgagcga tgcgagaacg cagcggcaag cctcgtttca gatcttattg
     1501 agtgccgaag tggtggaatg gcagacacgc acgtttgagg ggcgtgtggc gaaagccgtg
     1561 cgggttcaag tcccgccttc ggcaccaa
//
LOCUS       DATMTI010000218         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_645646, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000218 DATMTI010000000
VERSION     DATMTI010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645646"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VN039_03705"
     CDS             <1..588
                     /locus_tag="VN039_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWV45123.1"
                     /translation="GKASRQEVHHLRRCVDGVLIGVDTVLVDDPSLTARTGPRLEKLA
                     LQQPLRIVVDSRLRIPLKVQVLTQQAKAKTIIATTAAAPAARRSVLQKKGIEILTLPA
                     LQGRVSLPALLKQLGRRGITSLLVEGGSEVNAAMLKAKLINHIRLYMAPLLLGGRNAK
                     GVIGGESPARLAGAIEIQHVAIRSVGDDVVVEGDL"
     gene            585..1871
                     /locus_tag="VN039_03710"
     CDS             585..1871
                     /locus_tag="VN039_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45124.1"
                     /translation="MTGWIRFISLTLVLSSGTNSSVAEESVPVVENLTSLVLQYIDAH
                     GDEAGRLLQRILLDERASVETVSQIIKAERTYQSQPVGNLPEEQIVIRGQPYPLSLFI
                     PPTYQTSRAYALVVCLHGFGFTGEEYLERWRPRLGEDYLLACPSYPSGAWFTRRAEEL
                     VLETIRQVAHRYHIDPDRVFLTGMSNGGIGTWLIGMHHAPLFAGLAPMASGLDDVLMP
                     FLANLRSTPIYIIHGAKDQVMPVDLSRSIVRELDKLGYPYVYREHQREHPMAGGHYFP
                     REELPDLVAWFNRQRREPLPTSLTVVRDGSHFQPFNWVRLDATDPIAAFSDDLVDKRD
                     ERIKRRVYARLDASIAGSNRIEVKAEHVQRYSLFLNEQLIDVSKPLTVVTNGRLSFEG
                     TVTPSVETLLRQARLRQDPERLFPVHLIIDITKQAP"
     gene            1868..2683
                     /locus_tag="VN039_03715"
     CDS             1868..2683
                     /locus_tag="VN039_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45125.1"
                     /translation="MISAWRVRSGPVAHMLVVWIAMGIVVFAGDARSETCTLSPVHAG
                     LTLPIERVDPVRICRLQAIIQSYTTVSKVGPIRTALSESMYRHLLDHPPFTADVIRRL
                     HLGLYRSEARGPGRFWGDDGEGTKGIVELVYEDPTFRIYLLEGIHESRLLPHVTGKAV
                     VFLRIGVMRDANSHEATDSTLVAYTKLDNRFLSGLVSLLHPLVSRVVTSRLQKGVETV
                     DRLGVVMRLDPQRVMSVLTNPPPLPEHHLAFLKQTLIGRPSTDKGSSDGRSIP"
     gene            2572..>3329
                     /locus_tag="VN039_03720"
     CDS             2572..>3329
                     /locus_tag="VN039_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV45126.1"
                     /translation="MDESAAFTGTSPGVSETDANRPTEHRQGQLGRQIDPMTTSRSFT
                     LICTVGIFCFISYNMVRMPALSLFAESLGASPERIGLIVSVSTLTGVLLKLPSGALSD
                     IYGRRFLLRIGVVAFGLPPFLYPFITNLDALTALRFLHGFATAIFAPSALATVAELYR
                     ERRGAALGTYTACTQSGSLLGPFLGGYLVNAAGFPTAFVTAGAFGCIAMVLFYSLHLD
                     VSVPQRKEQGTAVVLSEMWKGFAAVAKNSKVLIT"
BASE COUNT          666 a          918 c          980 g          765 t
ORIGIN      
        1 ggcaaggcgt ctcgtcagga ggtgcatcac cttcgccgtt gtgtcgatgg cgtcttgatc
       61 ggagtagaca ccgttctggt tgatgatccg tcactgacag cgagaacggg gccgcgactg
      121 gaaaaattgg cattgcagca gcctcttcgc attgtcgtcg atagccgact gcgtataccg
      181 ctcaaggtac aggtcctgac acaacaagcc aaggccaaaa cgattattgc cacaactgct
      241 gcagccccag cggctcgacg atcggtcctg caaaaaaagg gcatagagat cctcacgttg
      301 cccgccctac agggccgggt atcgttgccc gctctgctca agcagctcgg tcgacgcggt
      361 atcacatcct tactcgtgga gggaggtagc gaggtcaatg cggcgatgct gaaggcaaaa
      421 ttgatcaatc acattcgcct ttatatggcg ccgctgcttc tcggcggacg gaatgccaag
      481 ggtgtgatcg ggggagaaag tccggcacgg ctcgcaggtg ccattgagat acagcatgtg
      541 gcgatacggt ccgtcggcga tgatgttgtg gtggaagggg atctgtgacc ggctggataa
      601 gattcatcag cttgacgctg gtccttagta gtggaacgaa ttccagcgtt gcagaggaat
      661 cggtaccggt cgtcgagaat ctgacttcgc tcgtcctcca gtatatagac gcccatggtg
      721 atgaagccgg tcgattgttg cagcgcattc tcctcgacga acgcgcatct gtcgaaacag
      781 tctctcagat cataaaggcc gaacgaacgt atcagagcca accagtcggg aatcttcccg
      841 aggaacagat cgtcatccgg ggacagccgt acccgctgtc cctgttcatc ccgccgacgt
      901 accaaacttc gagggcctat gccttagtcg tgtgcctgca cggattcggt tttaccggcg
      961 aggaatattt ggagcgatgg cgaccacggt tgggtgagga ctatctattg gcctgtccct
     1021 cctatccctc cggtgcgtgg tttacaaggc gtgcggagga gctggtgctg gagacgatcc
     1081 ggcaagttgc gcatcggtat catatcgacc ccgatcgggt tttcctcacg ggcatgtcaa
     1141 atggcgggat cggtacgtgg ctgatcggca tgcatcatgc gccgttgttt gccggactcg
     1201 cacccatggc gagcggactc gatgacgtgc tgatgccgtt tttagcgaat cttcgaagca
     1261 caccgatcta tatcattcat ggggccaaag atcaggtgat gccggtggac ctgagccgct
     1321 cgatcgttcg agagctggat aagctcggct atccatatgt gtatcgcgag catcaacgtg
     1381 agcatcctat ggcaggcggg cactatttcc ccagagaaga gttgccggac cttgttgcct
     1441 ggttcaatcg tcagcgccgt gaacccttgc cgacaagcct gaccgttgtg cgcgacggga
     1501 gccactttca accgttcaac tgggtccgcc tcgatgccac cgatccgatc gcggcatttt
     1561 cagacgattt ggtggacaaa cgcgacgaac gaatcaagcg tcgggtgtat gccaggcttg
     1621 acgcgtcgat tgccggaagc aatcgcatcg aggtgaaggc cgagcacgtt cagcgctaca
     1681 gcctattcct gaacgagcaa ttgatcgatg tctccaaacc gctcacggtg gtgacgaacg
     1741 gccgtctctc attcgaagga accgtcactc catcagtgga aaccctattg cgccaagcca
     1801 gactgcgaca agaccccgag cgcctctttc ccgttcatct cataattgac atcacgaagc
     1861 aggctccatg atctccgcat ggcgggtccg gtctggtcct gtggcgcaca tgcttgttgt
     1921 gtggatcgct atggggattg ttgtatttgc cggggatgcc cggtcagaaa catgtacgtt
     1981 gtcccccgtc catgccggac tcacacttcc catcgagcga gtggatcctg tccggatctg
     2041 tagactgcaa gccatcattc aaagttatac gactgtgagc aaggtgggac cgatccgaac
     2101 cgctctctct gaatcgatgt atcggcatct tctggatcat ccgcccttta ccgcggatgt
     2161 gatccggcgt ctgcatcttg ggctttaccg gtcggaagcg cgaggtccgg gccgtttctg
     2221 gggcgatgac ggcgagggga cgaagggcat cgttgagctg gtgtatgagg atcccacgtt
     2281 ccgcatctac ttacttgagg gaattcatga aagccggttg cttcctcacg tgacgggaaa
     2341 agcggtcgtc tttctcagga taggcgttat gcgagacgcg aatagccatg aagcgacgga
     2401 cagtacactg gttgcgtata cgaaattgga taatcgtttt ctttcagggt tggtttccct
     2461 cctgcatccg ctggtttcca gggtcgtgac aagtcggttg caaaaaggag tggagactgt
     2521 cgatcgattg ggggtggtga tgaggctgga cccccaacgg gtgatgtctg tattgacgaa
     2581 tccgccgcct ttaccggaac atcacctggc gtttctgaaa cagacgctaa taggccgacc
     2641 gagcaccgac aagggcagct cggacggcag atcgatccca tgacgacatc gcgcagcttc
     2701 acgctgatct gcacggtcgg catcttttgc ttcatcagct acaacatggt gcggatgccc
     2761 gcgctctcct tgttcgcgga atcgctcggc gcaagcccgg agcggatcgg tctgatcgtg
     2821 tccgtttcca ccttaactgg cgtcttgctg aaattgccct ccggcgccct ctccgatatc
     2881 tatggaaggc ggttcctgct gcggatcggt gtggtggcgt tcgggctgcc gccatttctc
     2941 tatcccttca ttacgaactt ggacgcactg acggcgctac ggtttcttca cggcttcgca
     3001 actgctattt tcgccccgag cgctcttgct actgtggcgg agctgtatcg ggaacggcgt
     3061 ggtgcggcgc tcgggaccta tacagcctgc acgcaatccg gctctctctt gggccctttt
     3121 ctcggaggat acctcgtcaa cgcggcaggg tttcccacgg cgtttgtcac ggccggcgcc
     3181 ttcggttgca tcgccatggt gttgttctat agccttcatc tcgacgtctc ggtgccgcag
     3241 aggaaggaac aggggacggc tgttgtgctg tccgaaatgt ggaaagggtt tgccgccgtc
     3301 gccaaaaaca gcaaggtgct gatcaccag
//
LOCUS       DATMTI010000219         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_730203, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000219 DATMTI010000000
VERSION     DATMTI010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730203"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VN039_03725"
     CDS             <1..403
                     /locus_tag="VN039_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45127.1"
                     /translation="KDIPTDRPTPKAEALVQLAWSRINKVAWNRRLHDPNSVRAYEDA
                     RDSSAIAELPLDKFFAEYTMAYSMIFMPNYGDKNRMLGHLTEAKRWFDEIPGKDDEVW
                     RYFLHSELLKAVLDADPMFQPILASAQQPKG"
     gene            476..1156
                     /gene="aat"
                     /locus_tag="VN039_03730"
     CDS             476..1156
                     /gene="aat"
                     /locus_tag="VN039_03730"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWV45128.1"
                     /translation="MNRHELCFPPVDRASPEGLLAVGGDLCPERLLEAYRHGIFPWYN
                     DDQPILWWSPDPRAVLFPNKLHVSHSLKRRLRPDVFTVTLDTGFRNIMEQCAGPRPQY
                     PQGGTWITGDMLDAYIRLHELGYAHSVETWQDGRLVGGLYGVAIGGAFFAESMFTRVD
                     DASKVALVRLVKQLQAWNFRLIDCQQPSPHVSRFGAEEIPRSDFIKHLNQALTLPDRR
                     GRWTFETV"
     gene            1266..1586
                     /locus_tag="VN039_03735"
     CDS             1266..1586
                     /locus_tag="VN039_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWV45129.1"
                     /translation="MSFAITPKDLKARLDKGDNLVLLDVREPWENQLAKLDNSILIPL
                     GTLPNSLSKLDRNAEIIAYCHHGMRSGDATGFLLQQGFSNVKNLIGGIDAWSVQVDGA
                     VPRY"
     gene            complement(1673..>2615)
                     /locus_tag="VN039_03740"
     CDS             complement(1673..>2615)
                     /locus_tag="VN039_03740"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV45130.1"
                     /translation="VHSGGPMKVLVTGGAGFIGSHVVDRLVEEGHQVVIVDNLATGKR
                     KNVNRAASLYKTDITSWRLERVFRNERPNIVLHLAAQISVRRSVDDPVFDAQINVLGT
                     MNVLQQAVRYGCRKVVFSSSGGAIYGEQETFPAAESHASNPLSPYGISKLCGEHYLFY
                     FQRTSGIPVVSLRYANVYGPRQDPEGEAGVIAIFIQKMLNNEQPIINGNGRQTRDFVF
                     VDDVAEANLAAMGQSAHGVYNVGTGTETSVNELFRMLAGLTASSAKEVHGPAKAGEQI
                     RSVIDPSRIKQELGWEVKVDLAEGLKQTVEFFQGKIR"
BASE COUNT          566 a          757 c          690 g          602 t
ORIGIN      
        1 caaagacatt ccaaccgatc gaccgacgcc caaagccgag gcgctggtgc agttagcctg
       61 gtctcgtatc aacaaggtgg cttggaacag gaggttgcac gacccgaaca gtgttcgagc
      121 ctacgaggat gcccgcgact catcagccat cgccgagctg ccgctggaca agtttttcgc
      181 ggaatacacc atggcttaca gcatgatttt catgccgaac tatggagata aaaaccggat
      241 gctcggacat ctcaccgagg ccaaacgttg gtttgacgaa ataccaggaa aagatgatga
      301 ggtctggcgc tatttcctcc atagcgaatt actgaaggcc gtactcgatg ccgatccgat
      361 gtttcagccc atcctggctt cggcgcaaca acctaaggga taatggccga tctcaagttc
      421 atctcggcat agtctttttg ccctgtttca cgtatcgtac ggggtgatgt tccgcttgaa
      481 tcggcacgaa ctttgttttc caccggttga tcgggcatcc cctgaaggtc tcctcgcggt
      541 aggaggcgat ctctgtccgg agcggttgct cgaagcctat cggcatggca tctttccctg
      601 gtacaacgac gatcagccca tcctctggtg gtcgccggat cctcgcgccg tcctgtttcc
      661 taacaagctc cacgtctcgc acagcctcaa gcgacggctt cgccctgacg tgtttaccgt
      721 cacgctcgat accggctttc ggaatatcat ggaacagtgc gcagggcctc gcccgcagta
      781 tccccaagga ggaacgtgga tcacgggtga catgctcgac gcctatatac gtttgcacga
      841 actcggctac gctcattccg tcgagacatg gcaggacggt cgtctggtcg gcggactcta
      901 cggcgtggcg atcggagggg ccttctttgc cgaatcgatg tttacacgag tcgatgatgc
      961 atccaaagtg gcgctcgtcc ggctcgtcaa gcagctccag gcctggaact tccgcctcat
     1021 agattgccaa cagccttcac cccatgtcag ccggttcggc gcggaagaga ttccacggtc
     1081 ggacttcatc aagcatctca accaggccct cacccttccc gaccgacggg gacgatggac
     1141 tttcgaaacg gtgtgagcca tagaatgaac aagatcgacg caccgccact tggatggcga
     1201 agcgcatttg acagcctttt cttccgtcgc ttatgattcc gttttagtca cgaggaggat
     1261 atgacatgag ctttgccatt acacccaaag acctgaaggc ccggctcgat aaaggggaca
     1321 acttggtgct tctggatgtg cgagagcctt gggaaaacca actcgccaag ctggacaatt
     1381 ccatcctcat tccgctcggc acactgccca actccctttc caaattggac agaaacgctg
     1441 agattatcgc ctactgccat catggcatgc gaagcggaga cgcgacgggg tttttgttac
     1501 agcaggggtt ttcaaacgtg aagaacctga tcggcgggat tgatgcctgg tctgtccagg
     1561 ttgatggggc agttccccga tactgatccg gtcagatacc gataggggaa aggccggcgg
     1621 tcgaatgctg accgcctcgt ggttcaagta gagattggct ttatacgcgc agctacctga
     1681 ttttcccctg aaaaaattcg accgtctgct tcaacccttc ggcaagatcc accttgacct
     1741 cccacccaag ctcttgtttg atccttgacg ggtcgatcac actgcgaatc tgctcaccgg
     1801 ctttggccgg gccatgaact tccttggcac tcgacgcagt caggccggcg agcatacgga
     1861 acaattcatt gacggatgtt tcggtaccgg ttccaacgtt gtacactccg tgtgcactct
     1921 gccccatggc ggccaggttc gcttcagcca catcgtcgac gaatacgaag tcgcgcgttt
     1981 gacggccgtt tccattaata ataggctgct cgttgttcaa catcttttgg atgaatatgg
     2041 cgataactcc tgcttctccc tcgggatcct gtctcggccc atacacgttg gcataccgga
     2101 ggctgaccac cggaatcccg cttgtccgct ggaagtagaa caagtaatgt tccccgcaca
     2161 gtttactgat gccatagggt gatagtgggt tgctggcgtg agattcagcg gcgggaaacg
     2221 tctcctgttc accgtagatc gctccaccgg acgaggagaa cacgaccttt cggcaaccat
     2281 accgaaccgc ttgttgtaac acgttcatgg ttcccaagac gttgatctgc gcatcgaaca
     2341 ccgggtcgtc taccgaacgg cggacactga tctgagccgc aagatggagg acgatgttgg
     2401 gccgctcatt gcgaaagacc cgttcaagac gccagctggt aatgtcggtt ttgtagagac
     2461 tcgccgcacg gttgacgttc ttccgctttc cggttgccag attatcgacg atgacgactt
     2521 gatgtccctc ttcaacgagt cggtccacca catgagaccc gataaaccct gcgccgcccg
     2581 tcacgagtac tttcattggc cctcctgaat gaact
//
LOCUS       DATMTI010000220         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_136224, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000220 DATMTI010000000
VERSION     DATMTI010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136224"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VN039_03745"
     CDS             <1..610
                     /locus_tag="VN039_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45131.1"
                     /translation="PADWYWYVDPATNTLHFAEASASADITLIKGRHLNSLEIEATKE
                     DIKNVVYFTGGDDGTGTNTNVFVLETATKGSNRFGLSTLSDNRISAAAAGSLAAAQAL
                     ARLLAQNELSVNASETYITNVVIIDGTMDISSISLGMMVGFSGFGTFVDRLLLQVVSI
                     TREPEQVTLGLGTLPKRSARAVADVQTALTYQQTIDNPAQPS"
     gene            631..1509
                     /locus_tag="VN039_03750"
     CDS             631..1509
                     /locus_tag="VN039_03750"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HWV45132.1"
                     /translation="MKISQYPQDSAPLTTDIMVMVDQTNSATKKVAISDLIALFTTSL
                     LPSGVMTEFAGKTAPSGWLLCYGQAISRTTYAGLFAALVPTVGTPTVTIASPGVFTLT
                     GHGLQIGDAVYLTTTGALPTGLSANTLYYVAATSFAANTFTLTASRGGSAINTSGTQS
                     GTHTLHASPHGIGDGSTTFNLPDMRGRSPSGADAMGGTAASRVTSGGSGIYGAAVGAT
                     GGEETHTLTSTEMPSHSHTQQVTHGTSGGYGFVDSTAASSSGTATSPTNTATAGSGGA
                     HNNMEPTTILNYIIKT"
     gene            1533..1724
                     /locus_tag="VN039_03755"
     CDS             1533..1724
                     /locus_tag="VN039_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45133.1"
                     /translation="MNNEDENLQITVELVEDEHTTIKTREGNANIAPGNYIATDQNGD
                     KFGITGSDLKALYSLAKAV"
BASE COUNT          472 a          542 c          445 g          384 t
ORIGIN      
        1 cccggcagat tggtattggt atgtagaccc ggccacgaac accctgcact tcgctgaggc
       61 cagtgcttcg gccgacatca cactcatcaa gggtcggcac ctcaacagtc tcgaaatcga
      121 agccacgaaa gaggacatca agaacgtggt gtacttcact ggtggcgatg acggcaccgg
      181 cacgaacacc aacgtcttcg tgcttgaaac tgctacaaag ggcagtaaca ggttcggtct
      241 ttcgacactc tctgataacc gtatctcagc agccgcagcc ggtagtttgg ccgcagctca
      301 agcactagca agactgctcg ctcagaacga actatcagtc aatgcttcag agacctacat
      361 caccaacgtc gtgatcatcg acggcactat ggacatcagc tcgatcagcc tcggcatgat
      421 ggtcggtttt tcaggcttcg gcacgtttgt cgatcggctg ctgcttcagg tcgtctcgat
      481 cacccgagaa cccgagcaag ttacgcttgg cctcggcacc ctgccaaagc gatcagcccg
      541 agcggtcgca gacgttcaga cagccctgac atatcagcaa acgatagaca accctgcaca
      601 accgagctag aataatcgta agaggaaaat atgaaaatat cgcaataccc acaagatagt
      661 gcaccactga ccaccgacat tatggtcatg gttgatcaaa ccaactcggc caccaaaaag
      721 gtcgctatct ctgacctgat cgctcttttc accacctcac tactgccctc tggcgtgatg
      781 acagagtttg ccggtaaaac tgcaccgtct ggctggctgc tctgttacgg ccaagcaatc
      841 agccgaacta cctatgccgg tttgtttgct gctctcgtac cgactgtcgg cactccgact
      901 gtcaccatcg caagccccgg tgtcttcacg ctgaccggcc acggcttgca gatcggcgac
      961 gctgtttatc ttacgactac tggtgccctg ccgactggct tatcagcaaa tacgctctac
     1021 tatgttgctg caaccagctt cgcagccaac acgttcacgc tgaccgcttc acgaggtggc
     1081 tcagcaatca acacctctgg cactcagagc ggtactcaca cgcttcacgc ttcgcctcat
     1141 ggtatcggtg acggttcaac tacattcaac ctgccagaca tgagaggccg cagcccgagc
     1201 ggtgctgacg ctatgggtgg tactgccgca agtcgtgtca cctctggtgg ctcaggtatc
     1261 tatggtgctg ctgtcggtgc aaccggcggc gaagagacgc acaccctgac atcgaccgaa
     1321 atgccgtctc actctcacac tcagcaggtt actcacggta cgtctggcgg ctacggcttc
     1381 gtcgatagta ccgctgcttc aagctcaggt acggcgacca gcccgaccaa caccgcaact
     1441 gccggtagtg gtggtgctca caacaacatg gaaccgacaa ccatcttgaa ctacatcatc
     1501 aaaacttagg gggaactatg gcagatactt acgtgaacaa tgaagacgaa aacttgcaga
     1561 tcactgtcga gcttgttgag gacgagcaca ccacgatcaa gacccgagag ggcaacgcca
     1621 acatcgcacc cggtaactac atcgccactg atcagaacgg tgataagttc ggcattaccg
     1681 ggtctgacct caaggcacta tatagcctag cgaaagctgt ctgataactt attgcagcga
     1741 atactagacc tacgtataat acttacgcta tcataatggg gaaaacggta ttatgaagat
     1801 caacaggtac gatatagact tatggacgag caaccaaaga aga
//
LOCUS       DATMTI010000221         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_179178, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000221 DATMTI010000000
VERSION     DATMTI010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179178"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1596)
                     /gene="asnB"
                     /locus_tag="VN039_03760"
     CDS             complement(<1..>1596)
                     /gene="asnB"
                     /locus_tag="VN039_03760"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWV45134.1"
                     /translation="EAGRQPMLSSDGNIGVVFNGAIYNFLELRKELESDGYAFISHTD
                     TEVLIQGYRRWGIDALVTRLRGMFAFGIWDNALQKLYLVRDRLGVKPLVFSARGGVMV
                     FASTVRALRQAGFVHELDEHAIAEYLQLGFIPDDYSIYREARKLPPASILEWHGGKVT
                     IRRYWSSPPVTPSSLSFEESVESAEEQLLKAVELRLHADVPIGILLSGGIDSALICWA
                     ATKLGRRITAYTIGTPGDPWDETAAARSTAEALGLDHRVLDMGQDDQYEMNDLVSAFA
                     EPFACSSALGMLKVSKYVAASSKVLLTGDGGDDVFLGYPRHRHVLIAGKLAPIIPYSL
                     RKWWCKDGSLLPKVGPLCRVATFLDYVAESPTIHGRSVMKSKILGDRLKNGMDNYGAW
                     VPSMTGSLISHFLNYEYRNRFVGEYLTKVDGATMHYGLEARSPFLDQDMWEFASSLPY
                     ALRLHRWDLKAILREVARRRISPQLAEGRKRGFGIPVHRWIVGPWRPLVEASLGESIL
                     EKEGWIRPKASSELFQSALKQGEA"
BASE COUNT          404 a          465 c          364 g          363 t
ORIGIN      
        1 cgcttcgccc tgctttaatg cggattgaaa caattccgat gaggctttgg gccggatcca
       61 gccttctttc tccaaaatcg actcccccag ggatgcttcg accaggggac gccaaggacc
      121 aacgatccaa cgatggacgg gaataccgaa ccctcgtttc cttccttcgg cgagttgtgg
      181 actgattctt cgtctagcga cttcgcgcaa gatcgcctta agatcccaac gatgcaatct
      241 tagtgcatat ggcaatgagg aggcaaattc ccacatatcc tggtccagga acggtgatcg
      301 tgcttcaagc ccatagtgca tcgtcgctcc atccaccttt gtgaggtact ctccaacgaa
      361 gcgattccga tactcgtaat tcaggaaatg actgattaat gatccggtca tggacggtac
      421 ccaagcgcca taattgtcca taccgttctt caggcgatcg ccgaggatct tcgatttcat
      481 tactgaacgt ccatggattg tcgggctttc agcaacataa tccaagaacg tcgctactct
      541 gcaaagcggt ccgacctttg gaagcaatga accgtcttta caccaccact tccgcagtga
      601 gtatggaata attggtgcca gttttccagc tatcaagaca tggcgatgac gcgggtaacc
      661 caaaaacaca tcatctcccc catcacccgt caaaagaacc ttggaggatg cggcgacata
      721 ttttgagacc ttgagcattc caagcgccga tgaacaagca aaaggttcgg caaaggctga
      781 gactaggtca ttcatttcat actggtcatc ttgccccatg tccagcacac gatgatccag
      841 tcctaaagct tccgccgtgc ttcttgctgc cgcagtctcg tcccaaggat ctccaggtgt
      901 gccgatcgtg tacgcggtaa tccttctacc gagtttcgtc gcggcccagc atatcaaggc
      961 agaatcgata cctccgctaa gcagaatccc gattggaaca tccgcatgca atcgcagctc
     1021 cactgccttg aggagttgct cttcagccga ctcgacggat tcctcaaatg acagggaaga
     1081 tggagtcaca ggaggagacg accagtatct gcggatcgta acttttccac catgccattc
     1141 caaaatcgat gcaggaggta atttgcgagc ctcccggtat atcgagtaat catcgggaat
     1201 aaatcccagc tgcagatatt ctgcaatcgc gtgttcatcc aactcatgga cgaacccggc
     1261 ttgcctgagt gcacgaaccg ttgaggcaaa gaccatcacg ccacctcgtg cactgaaaac
     1321 aagcggcttg accccaagcc gatctcgaac gaggtacagc ttctgcaatg cgttgtccca
     1381 aattccaaac gcaaacatcc cccggagtct cgtcacgagc gcgtcaattc cccacctacg
     1441 atagccctga atcaacacct ccgtatcggt atggcttata aaggcatacc cgtcagactc
     1501 aagctctttc cgcaattcaa gaaagttgta gatggccccg ttgaagacca cgccgatatt
     1561 accatcggat gagagcatcg gctgcctacc cgcctc
//
LOCUS       DATMTI010000222         4199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_772760, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000222 DATMTI010000000
VERSION     DATMTI010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772760"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VN039_03765"
     CDS             <1..1254
                     /locus_tag="VN039_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45135.1"
                     /translation="DANAAAQLDELRGAQQACRAAEQTHEVLKEEIRVLREHIAQLNE
                     GLADRDHLRAKVKKLASGQDRVHQLEVELSDREAAHRGTLQQLEGAIAERDRRISEID
                     ANAAAQLDELRGAQQACRAAEQTHEVLKEEIRVLREHIAQLNEGLADRDRLRAQVEKL
                     ESLQDRVHQLEVELSDREAAHRGTLRQFERSVAERDRRIEELVPATHLLREKGAEIKE
                     WDKKYARTVQEYKTEMTKLQEQCTAQEQLREQLLLNEQQLHERDEQIASLHRQLQDLQ
                     AERHDLLQQVQNIPGKDEQIDRLHKRLRELRSAHRASAAPATAGSRQSSQNGVGSNAQ
                     VRQSKAGKNVQEDDLKKIYGIGSVFADTLNKMGTRTFIQIARWKPEDIEKIAKKLDAD
                     PERIKRENWIADAKKQHYRKYGERV"
     gene            complement(1259..1390)
                     /locus_tag="VN039_03770"
     CDS             complement(1259..1390)
                     /locus_tag="VN039_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45136.1"
                     /translation="MKLSTQNVLPWSVIAIAGWLTDLIWRTRQGRHFIETPVKNRVL"
     gene            1800..2654
                     /locus_tag="VN039_03775"
     CDS             1800..2654
                     /locus_tag="VN039_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV45137.1"
                     /translation="MRRAGILTGGVVALILLAVVCIPRHLSSPRPAVTHASFHASVEQ
                     GGLVLRGSLPNERSKATILEQAHAFSAKTRMRVIDQFIVEKQVKAAAWVETIPQLLPI
                     LGLMVERGSIIIDGRSLVVSGQVAGHREKAEVLQAASPAIRAGLRIEDRVVVAPTRAS
                     SPAAVPLSALQLLLNQVLAKSSIEFEPKSAVITAKGQAVLDQIIVLLRRSPDTPIEIA
                     GHTDTVGKAEYNMQLSRRRAEAVKQYLTSHGLTNPFTPIGYGSTRPLSHEKQQPGLQQ
                     NERIELLI"
     gene            2669..>4199
                     /locus_tag="VN039_03780"
     CDS             2669..>4199
                     /locus_tag="VN039_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45138.1"
                     /translation="MITLMSQMVGCLLIAAGIGVAVGWLLRRLPKNSIGQPYEDVVNQ
                     LRTKEQALETISHDLKVKTSGMQILEQKIMSSETLYSSAQRELAARGERVKALEAELA
                     VRSKRLTDVEAEGVAARQRVSEVDASAAAQAEELRSAQQACRAADQAREAMEQEIRML
                     REHITQLNEGLADRDRLRAQVEKLASAQDRIHQLEVELSDREASHRGTIQQLERAIAE
                     RDRQVSEVDANAAAQADELRAAQQACQAADRTHEVLKEEIRVLREHIAQLNEGLADRD
                     RLRAQVKKLESAQDRIHQLEVELSDREASHRGTIQQLERSITERDRRIDELVPVTHLL
                     REKESELKEWERTYARTVQEHEMEVAKLQDQCAAQEQLQAQHLLDEQHLHERDEQMAG
                     FVRQVHDLQAERQSLLEEVQSIPGKDEQIERLHKRLREMRAALRANATPATAVSRQPR
                     QNGAKPSSPVGQSKVSKDGQEDDLKKIFGIGPAVAQTLHKMGTRTFIQIARWKPEDIA
                     KI"
BASE COUNT         1058 a         1187 c         1218 g          736 t
ORIGIN      
        1 gatgcgaatg cggccgccca gctggacgag cttcgcggag cacaacaggc ctgtcgagcc
       61 gccgaacaga cccacgaggt gttgaaggag gaaatccgcg tcctccgcga gcatatcgcc
      121 caactcaacg aggggctcgc cgaccgagac cacctgcgcg ccaaggtgaa aaagttggcg
      181 tcagggcaag atcgcgtcca tcagttggag gtggaactga gtgatcggga agccgctcat
      241 cgggggacac tccagcagtt ggaaggggcc attgcggagc gggaccggcg gatcagtgag
      301 atcgatgcga atgcggccgc ccagctggac gagcttcgcg gagcacaaca ggcctgtcga
      361 gccgccgaac agacccacga ggtgttgaag gaggaaatcc gcgtcctccg tgagcatatc
      421 gcccaactca acgaggggct cgccgaccgg gaccgactcc gcgcccaggt cgagaagctg
      481 gagtcgctgc aagaccgtgt ccatcagttg gaggtggaac tgagtgatcg ggaagccgcc
      541 catcggggca cactccggca gttcgaacga tccgttgccg agcgcgaccg ccgaattgaa
      601 gaactggtgc cggcaaccca cctccttcgc gagaagggag cagagatcaa ggaatgggat
      661 aaaaaatacg cgcgcaccgt tcaagaatac aagacagaaa tgacaaagct ccaagagcaa
      721 tgtacggcac aggaacaact ccgcgagcaa cttctgctca atgagcaaca gctacatgaa
      781 cgagatgagc agattgcgag cctccaccgt caattacagg atcttcaagc ggaacgtcac
      841 gatctcttac aacaggtaca gaatattccg gggaaagatg agcagatcga tcgtctgcac
      901 aaacgactca gagagctgcg gtcggcacat cgtgcgagcg cagctccggc aaccgcaggg
      961 tctcgccagt caagccagaa cggagttgga tcgaatgctc aagtcagaca gtccaaagcc
     1021 ggtaagaacg ttcaggaaga cgatctgaaa aagatctacg ggatcggatc ggtcttcgcg
     1081 gacaccctca ataagatggg cacccgcacg tttatccaaa tcgctcgctg gaaaccggag
     1141 gatatcgaaa aaatcgcaaa gaagctcgat gccgatcctg agcgcattaa acgagagaat
     1201 tggatcgctg acgccaagaa acaacactac agaaaatacg gggaacgggt atagcacctc
     1261 acaacacacg gttttttacc ggcgtctcta tgaaatgcct cccctgcctg gtccgccaaa
     1321 tcaaatccgt caaccaacca gctatggcaa tgaccgacca tggcaacacg ttctgtgtgg
     1381 atagcttcat ggtctaacat ctccgcggat cagctcagac tgaacagcaa acagagtgtg
     1441 cagccgcctt gccccagagg caaatcttac ccggcccgca cgattcatga acacttctgc
     1501 tgcaatcgcg gattcgctgc tgcgacaagc cttcggcggg caggctcgcc cctcgcagcc
     1561 tcgacatact gtttcaagta tgccttggtt cctcggagct ccacgtgcaa gacactgccg
     1621 gctcaccgac gttcacaaac atggcgctca ttactcttcg cgccgtggac ctcgctacgc
     1681 gactgcgcga tttcgccacg aactgtcatg aatcatgcag gctagtacat ttattatcaa
     1741 atgtggtacc gtgccgcgtc gcaccccaat aggcacatca tctacagcgg tctgatctca
     1801 tgaggcgtgc gggaattctc accggcggtg tcgttgcgtt gatactcctc gccgtggtgt
     1861 gcatcccgag gcatctttcg tcgccacggc ccgccgtaac tcatgccagc tttcatgcca
     1921 gcgtcgaaca gggcggcttg gtgcttcgcg gttcgttgcc gaacgaacgc agtaaggcca
     1981 cgattcttga acaagctcat gcattctccg cgaaaacgcg aatgcgtgtg atcgaccagt
     2041 ttatcgtcga gaaacaggtc aaagcggctg cgtgggtgga gacgattccg caacttttgc
     2101 cgatcttggg cttgatggtt gagcgtggtt caatcatcat cgacggacgt tctctggttg
     2161 tcagcggtca ggttgccggt catcgcgaga aggctgaggt attacaggcc gcatctccag
     2221 cgatacgggc gggcctgcga attgaagatc gtgtggtggt ggcgccgacg agagcatctt
     2281 ctcccgccgc cgttcctttg tcggcactcc agctgttgct gaaccaagtt cttgcgaaat
     2341 cctcgatcga gtttgagccc aagagcgccg tgatcacggc caaaggacag gcggtgctgg
     2401 atcaaatcat cgttctttta cgaagatctc ccgatacgcc gatcgaaatt gcaggccata
     2461 ccgataccgt tggcaaggcg gagtacaaca tgcagctgag ccggcgtcgg gccgaagcgg
     2521 tcaaacagta cttgaccagt cacgggctca cgaatccgtt taccccgatc ggctatggtt
     2581 cgacacgccc cctctcccat gagaaacaac aaccaggcct ccaacagaat gaacggatcg
     2641 aattattgat ctaagaatgg aatgacgcat gatcacactg atgagccaga tggtcggatg
     2701 tctccttatc gcggcaggaa ttggtgtcgc ggtcggttgg ttgttgcgga ggctcccaaa
     2761 gaattcgatt gggcaaccgt acgaggatgt cgtcaatcag ttgcgaacca aggaacaggc
     2821 gctggagacg atatcgcatg acttgaaggt gaagacttcg ggaatgcaga ttcttgaaca
     2881 aaagatcatg tcgtccgaaa cactgtactc ctcagcccag cgagagctgg ctgcacgcgg
     2941 cgaacgggtg aaggcgcttg aagcagaatt agctgtacga tcgaaacgac tgacggacgt
     3001 ggaagctgaa ggcgtcgcgg cacgccaacg cgtgagcgag gtcgatgcga gcgctgcggc
     3061 ccaggctgag gaactccgca gcgcccaaca ggcctgtcgg gccgccgacc aagcgcgtga
     3121 ggccatggag caggaaatcc ggatgctgcg ggaacacatc actcagctca acgaaggcct
     3181 cgccgatcgg gaccgactcc gagcccaggt ggagaagctg gcatcggcgc aagaccgcat
     3241 ccatcaactg gaggtggaac tgagtgatcg ggaagccagc catcggggca ccatccagca
     3301 actcgaacgg gctatcgctg aacgagaccg gcaagtcagt gaggtcgatg cgaatgcggc
     3361 cgctcaggcc gacgaactcc gcgcggccca acaggcttgt caggctgccg accgaacgca
     3421 cgaagtgttg aaggaggaaa tccgcgtcct ccgcgagcat atcgcccaac tcaacgaggg
     3481 gctcgccgac cgagaccggc tccgagccca ggtgaagaag ctggaatcgg cgcaagaccg
     3541 cattcaccag ctagaggtgg aactgagcga tcgggaagcc agccatcggg gcaccatcca
     3601 gcagctcgaa cggtccatca ccgagcgaga ccgtcgaatt gacgagcttg tgccggttac
     3661 ccacctcctt cgcgagaagg aatcagagct caaagaatgg gaaaggacat atgcgcgtac
     3721 cgttcaagaa cacgagatgg aggtggcgaa gctccaagac caatgcgctg cacaggaaca
     3781 actccaggcg caacatctac tcgatgagca acatttgcat gaacgagacg aacagatggc
     3841 gggcttcgtc cgtcaggtgc atgaccttca agcagagcgt caaagtctat tagaagaggt
     3901 gcagtctatt ccagggaaag atgaacagat cgaacgcctg cacaaacgcc tcagagaaat
     3961 gcgagcggca cttcgcgcga acgcgactcc ggcaaccgcc gtgtctcgtc agccccgtca
     4021 gaatggagcc aagccgagtt ccccggtagg acagtcaaag gtgagtaagg acggtcagga
     4081 agacgatctc aaaaagatct tcgggatcgg gcctgccgtt gcacagaccc tccataagat
     4141 gggcacccgc acatttattc aaatcgctcg ttggaaaccg gaagatatcg cgaagatcg
//
LOCUS       DATMTI010000223         1845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_772791, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000223 DATMTI010000000
VERSION     DATMTI010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772791"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..>1845
                     /locus_tag="VN039_03785"
     rRNA            60..>1845
                     /locus_tag="VN039_03785"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          477 a          439 c          577 g          352 t
ORIGIN      
        1 accttcaagc cctttgggca agaaggcgac actcaactga aaacgttggg tagggttgcc
       61 atcgcgcagc gatggcgcaa ccgccacaca agcagaaggc ttgaaggtgg ggcgactgcg
      121 taccttttgt ataatgggtc agcgacttac atgtctgtgg caagcttaac cgactaggga
      181 aggcgaaggg aaaccgagtc cgaacagggc gcatagtcgc aggcagtaga cccgaaacca
      241 ggtgatctag ccatgcccag ggtgaaggtg cggtaacacg cactggaggc ccgaacccac
      301 atctgttgca aaagatgggg atgaggtgtg gctaggagtg aaaagctaac cgaacctgga
      361 gatagctggt tctcctcgaa agctatttag gtagcgcctc gtgcgaacac tgttgggggt
      421 agagcactgt tatggctagg gggtcatcgt gacttaccaa accatggcaa actccgaata
      481 ccaacacgtg ctacacggga gacacacggc gggtgcgaac gtccgtcgtg aaaagggaaa
      541 caacccagac ccgcagctaa ggtccccaat taccgctcag tggaaaacga tgtggaaagg
      601 cacaaacagc caggaggttg gcttagaagc agccaccctt taaagaaagc gtaatagctc
      661 actggtcgag tcggtctgcg cggaagattt aacggggcta agcggtaaac cgaagctcgg
      721 ggtgcgtatg ttcttcggaa cgtatgcgcg gtagaggagc gttgtgtaag ccggtgaagg
      781 tgtgtcgaaa ggcatgctgg aggtatcaca agcgcgaatg ctgacatgag taacgataat
      841 ggaggtgaaa agcctccacg ccgaaagccc aaggtttcct agcgcaacgt tcatcggcgc
      901 agggtgagtc ggtccctaag gcgaggcgga aacgcgtagt cgatgggaat ctggttaata
      961 ttccagaacc gatcgtgatt gcgatggagt gacggagaag gttaggtgta ccgggcgttg
     1021 gttgtcccgg ggaaaggagg taggcgttga cggtaggcaa atccgccgtc atctacgccg
     1081 agcaccgaga cgagtccttt ttggacgaag tcactgatac cacgcttcca ggaaaagctt
     1141 ctaagcttca gatcacgagg accgtaccgt aatccgacac aggtaggcag ggtgagaatc
     1201 ccaaggcgct tgagagaact tgggtgaagg aactaggcaa catagcaccg taacttcggg
     1261 agaaggtgca cccgggatcg tggctacacg cgtagctgag cgattccggg tcgcagtgac
     1321 caggccgctg cgactgttta ccaaaaacac agcactctgc aaacacgaaa gtggacgtat
     1381 agggtgtgac gcctgcccgg tgctggaagg tcaattgatg gggtcagccg caaggcgaag
     1441 ctcctgatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
     1501 ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa cgacagcggc gctgtctcca
     1561 cccaagactc agtgaaattg aaatcgctgt gaagatgcag cgttcccgcg gcaagacgga
     1621 aagaccccgt gaacctttac tatagcttta cactgaacgt tgagttcttc tgtgtaggat
     1681 aggtgggagc ctttgaagcg atggcgctag ctgtcgtgga ggcaaccttg aaataccacc
     1741 ctgaagtgct tgacgttcta acctgggccc gtaatccggg tcggggaccg tgtatggtgg
     1801 gtagtttgac tggggcggtc tcctcccaaa gagtaacgga ggagc
//
LOCUS       DATMTI010000224         1666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_136311, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000224 DATMTI010000000
VERSION     DATMTI010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136311"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VN039_03790"
     CDS             complement(<1..443)
                     /locus_tag="VN039_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45139.1"
                     /translation="MAERFGEVDRRRIGNYVYEGYSQDLASRSKWEKRMEAAMDLALQ
                     LQKAKSFPWPGASNITFPLITIAALQFHSRAYPALVSAPDVVQCEVFGPDPDGKKTER
                     AERVSAHMSWQLLTQDQSWEEQQDRLLISVPIVGCAFKKYYHSAE"
     gene            complement(501..1355)
                     /gene="terL"
                     /locus_tag="VN039_03795"
     CDS             complement(501..1355)
                     /gene="terL"
                     /locus_tag="VN039_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HWV45140.1"
                     /translation="MARVLRPESTAWKSLLFRAHASFDDFSNALWPEQFPESRLFAIR
                     QAFIEDGDAAGYSQEYLNDPFDNSEAYLRKDDFIPMKEDDYEADKRICVGCDFAVSRS
                     DRANRTSFTVGGQDVNHRLHVIDQFKGRWDTLEWIEVLFDIQSKWQPEVFFVEDGVIW
                     KSVAPIVYKEMMRRGNGHWLNIQPINPTRDKATRGRDYQKMMRAGACRFDKKAGWYED
                     FEQENLRFTGNSDATLDDQFDSMALLAKGFNTLVDVEEEDFESEETIEMSRLDPRVST
                     GRSAVTGY"
     gene            1368..>1666
                     /locus_tag="VN039_03800"
     CDS             1368..>1666
                     /locus_tag="VN039_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45141.1"
                     /translation="MKDRPVHSNLASTAAAGKEGSIEPLAEFPATIEVFDLFVLFKVV
                     TDNEARALPFPLHPAHLLTRTLRDETTLVVVLKSNNDVCLGVGRKEGDSKLPLYV"
BASE COUNT          402 a          429 c          429 g          406 t
ORIGIN      
        1 ttttctgcac tgtggtaata tttcttaaac gcacagccaa caattggaac agagataaga
       61 agacgatctt gctgctcctc ccaggattga tcctgagtaa gaagctgcca gctcatatga
      121 gcagacacac gttcagctcg ctcggtcttc tttccgtctg gatcaggacc aaatacttca
      181 cattggacta catctggcgc ggagacgagt gcgggatatg cgcgggagtg gaattggagc
      241 gccgctatag taataagcgg aaaggtaatg ttggaggcgc caggccaggg gaaagacttc
      301 gccttttgga gctgaagggc gaggtccatt gctgcttcca tgcgcttttc ccacttcgag
      361 cgggaagcta agtcctggga atagccttca tagacgtagt taccaatcct ccggcgatcg
      421 acttccccaa agcgttcagc caagttcgga gactcgattg tctccttgtt gatagttatg
      481 cggtgatcca ggtgtagcat tcaatacccc gtcaccgcgc tgcgaccagt gctcactcta
      541 ggatcaaggc ggctcatttc aatagtttcc tcagactcga aatcttcttc ttcaacgtcc
      601 acaagagtat tgaagccttt ggctaggaga gccatggagt caaactggtc atcaagagtc
      661 gcatcggaat tcccggtaaa gcgaagattc tcttgttcga aatcctcgta ccagccggcc
      721 ttcttgtcaa agcggcaagc tccggctctc atcatctttt ggtagtcgcg tccgcgtgta
      781 gccttgtcgc gggttgggtt aattggctgg atattcaacc aatggccatt tcctcgccgc
      841 atcatctctt tatagacgat aggagcgaca gacttccaaa tcacgccatc ttctacgaag
      901 aaaacttcag gttgccactt tgactggata tcgaagagaa cctcgatcca ttcaagggtg
      961 tcccaacggc ctttaaattg atctataaca tgaagacgat ggtttacatc ctgcccgcca
     1021 acagtgaagc tagtacgatt agccctgtcg gaacgagaaa cagcaaaatc gcagccaaca
     1081 caaatgcgct tgtcagcctc ataatcgtcc tccttcatag ggatgaagtc gtcttttcga
     1141 aggtaggctt cggagttgtc aaaaggatcg ttgaggtatt cttgagagta accggcagca
     1201 tccccatcct cgatgaaggc ctggcggatg gcaaagaggc gggattcagg gaactgctct
     1261 ggccacaagg catttgagaa gtcatcgaac gatgcgtggg cgcggaaaag gaggcttttc
     1321 caggctgttg attccggccg aagaaccctc gccaagatgg aatcctcatg aaggatcgtc
     1381 ccgtgcattc gaaccttgcc tccacggcgg cggcagggaa ggagggctcg atagaaccac
     1441 ttgcggaatt tccggcgacg atcgaggttt tcgacctgtt cgtcctcttc aaggtcgtca
     1501 cagataatga ggccagggcg cttccctttc cacttcatcc cgcgcatctt ctgaccagaa
     1561 cccttcgcga tgaaacgaca ctcgtggttg tcctgaaatc gaacaatgat gtctgtcttg
     1621 gagtcggtcg gaaggaggga gactccaaac tccctctgta tgtctt
//
LOCUS       DATMTI010000225         1692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_136319, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000225 DATMTI010000000
VERSION     DATMTI010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136319"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..777
                     /locus_tag="VN039_03805"
     CDS             337..777
                     /locus_tag="VN039_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HWV45142.1"
                     /translation="MSTPASSESAILPPNAPAAAVQPVQDMVGSGKAWGLCTAVDLYD
                     CNPDLIRDANYIKRYVVELCALIGMKRYGECQVVNFGEGRVAGYSMVQLISTSLISGH
                     FANETNHAYLDIFSCKGYDPTVVESFSKEFFGARRSVATATLRY"
     gene            873..1211
                     /locus_tag="VN039_03810"
     CDS             873..1211
                     /locus_tag="VN039_03810"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWV45143.1"
                     /translation="MKTTTVKEVLDSLPSKLDKDAAEDLDAVYQFDLRGNQGGQYHLL
                     VQNGTCVVRDGTHADPHVTLSMTGEDCIGVLNGQFSGMTMAMSGRLQVTGDIGLAMQL
                     KSLFPNVVES"
     gene            complement(1218..>1692)
                     /locus_tag="VN039_03815"
     CDS             complement(1218..>1692)
                     /locus_tag="VN039_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV45144.1"
                     /translation="PFIIMFSVPMGFPGVILILFLTNTTLSTTSMMGIIMMFGIVVSN
                     GVLLVDYTNVLRRRGESLHLAVVIAARTRLRPILMTSLATVFGLLPMAIGLGTGGETA
                     PLSRAVIGGLSVSTLLTLFLVPTMYAILEERFPRRLDLEMRDQRQAQAGGVSVPE"
BASE COUNT          407 a          439 c          463 g          383 t
ORIGIN      
        1 agcttggatc caagccgggt ggtgtgttga aagatgcatt tggggcaggt gtaatggatg
       61 aactggttgc cgcccaccag gcgcttcctc atgaggactt tgcaccaagt gcagaggcga
      121 tcaggtaact cgatccggga tgtgttagag gcgaatggca tgaggcatat tgttcctgag
      181 ctccgtgacc ttgtcaatca attggtgatg aggggtccct tttcaaaagc cgaaatattc
      241 ctcacggttc ttattcatag caagagaatt tatggtatag ttcacgcatg atcgttcctg
      301 tccatacaac cttttacaga aagggatcaa ccccctatga gtaccccagc cagttccgag
      361 tccgcgatct taccacccaa cgcccctgcg gctgctgtcc aacctgtcca agacatggtg
      421 ggaagcggca aggcatgggg gctctgcacc gctgttgatc tctacgattg caatcccgat
      481 cttatacgtg atgcgaatta tatcaagcgc tatgtggttg agctctgtgc gctcatcggc
      541 atgaagcgct acggtgaatg ccaggtcgtc aatttcggcg aaggccgtgt agccggctac
      601 tcgatggtgc aactgatctc aacctcattg atcagcggcc attttgccaa tgaaaccaat
      661 cacgcctacc tcgacatttt cagctgcaag gggtatgatc ccaccgtcgt cgagtcgttt
      721 tcgaaggagt ttttcggcgc ccgtcgtagt gttgcgaccg ccacacttcg gtactaaaac
      781 ttttgttcac agggatcttg ctttgggggc gctaggttag tggagcgccc ccgaatactt
      841 taaccatcca atgccatagg tatagccaag acatgaaaac cacgaccgtc aaagaagtgc
      901 tcgactcact tccgtcaaaa ctcgataaag atgcggctga ggatcttgat gctgtctacc
      961 agttcgacct ccgaggaaac caaggcggcc agtaccatct gctggtgcaa aatgggacct
     1021 gcgtcgtgag ggatggaacc catgccgatc cgcatgtgac cctctcgatg accggagaag
     1081 actgcatcgg ggtcctaaat gggcagttca gcgggatgac gatggcgatg tcggggcgtt
     1141 tgcaagtcac cggggatatc gggctggcta tgcagctgaa atccttgttc ccgaacgttg
     1201 ttgagagtta acgagcatta ctccggaaca gaaacccctc cggcctgagc ttgccgctga
     1261 tctcgcattt ccaaatcaag tcttctcggg aaccgttctt ctaaaatcgc gtacatcgtt
     1321 gggacgagga acaacgtgag gagtgtagag acgctcaatc ctccgatgac cgctcgggac
     1381 aggggagcgg tttcaccgcc ggttccaagg ccgatcgcca tgggaagaag gccgaagacc
     1441 gtggccaggg aggtcatgag aatcggacgc agtctagttc gcgcggcgat cacgaccgcc
     1501 aggtgaagtg attcccctct gcgcctcaac acgttggtgt aatcgaccaa caggacaccg
     1561 ttggaaacga caatcccaaa catcatgatg atgcccatca tcgatgtggt ggacagcgtc
     1621 gtattcgtca gaaacagtat caaaatgacc ccagggaagc ccattggcac tgaaaacatg
     1681 atgatgaaag gg
//
LOCUS       DATMTI010000226         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_391226, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000226 DATMTI010000000
VERSION     DATMTI010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391226"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VN039_03820"
     CDS             <1..348
                     /locus_tag="VN039_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45145.1"
                     /translation="ALSYHRERKPVHLFCVRDRGSYRDVYDQEKKFFLDALTAHSDIE
                     TQLAMEYVNRCRFILTTRMAEDDVTDEGYDFNGWILEFYQEQCNGIVQIDKQGFYSPK
                     GELIVDMSVSGED"
     gene            338..1009
                     /locus_tag="VN039_03825"
     CDS             338..1009
                     /locus_tag="VN039_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HWV45146.1"
                     /translation="MKTNVLGTFPFPSTLFEKTARWFERVNASLLESLPCTQGCSDCC
                     VGLFPVTILDRWEIQRGLRSLADEHRKRIERTAAEQIAMLMIAAPQLRTNRFIDQWKE
                     KDIDRVSEQFQTWPCPALEVDGTCGLYEFRPLVCRSMGVPQDDGVLVNGACAVQTAVP
                     LIRPSKVFREEENHLAGMEAVEIEALRLQAGIMGEELFLPYAFIRDPDVGELESGLGQ
                     RRIPN"
     gene            1077..1153
                     /locus_tag="VN039_03830"
     tRNA            1077..1153
                     /locus_tag="VN039_03830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1111..1113,aa:Arg,seq:ccg)
     gene            1246..1321
                     /locus_tag="VN039_03835"
     tRNA            1246..1321
                     /locus_tag="VN039_03835"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1279..1281,aa:Lys,seq:ctt)
BASE COUNT          404 a          489 c          505 g          432 t
ORIGIN      
        1 gccttgagtt accatcgaga gcgtaagccc gtccacctct tttgcgtacg tgatcgcggc
       61 agctatcgag atgtctacga tcaggaaaaa aagttttttc tggacgctct caccgctcac
      121 tccgatatcg aaacacaact tgcgatggaa tatgtgaatc gctgtcgatt tatcctgacg
      181 acgcgtatgg cggaggatga tgtgaccgac gaaggctatg atttcaatgg atggatcctc
      241 gagttctatc aggagcagtg caacggaatc gtccaaatcg acaagcaagg tttctactca
      301 cccaaaggtg agctcatcgt ggatatgtcg gtctcgggtg aagactaacg tgctggggac
      361 gtttcccttt ccttcaacgt tgtttgagaa gactgctcga tggtttgagc gggtgaatgc
      421 ctctctcctc gagagccttc cttgcaccca gggctgttcc gattgttgtg tcggcctgtt
      481 tcccgtgact atcctggacc ggtgggaaat tcaacgcggt ctccgaagtc tggctgatga
      541 gcaccggaag agaatcgaac ggacggcagc ggagcaaatc gctatgctaa tgatcgccgc
      601 accgcaactg aggacgaatc gttttatcga ccagtggaag gagaaggaca ttgatcgagt
      661 cagcgaacaa ttccagacgt ggccctgtcc ggcgttggag gtggatggaa cgtgtggact
      721 ctatgagttt cggccgctgg tctgtcgttc aatgggcgtt ccccaggatg acggagtcct
      781 ggtcaatggc gcttgtgctg tgcaaacggc cgtgccactg attcggccct caaaagtctt
      841 ccgggaggag gagaatcatc ttgctgggat ggaagcggtg gaaatcgagg ccttgcgcct
      901 ccaagcagga atcatgggtg aagagctgtt cctgccctat gctttcatcc gggatcccga
      961 cgtgggagag ctggagtctg gattggggca gcggcgaatt cccaactgag gtaacacgat
     1021 gcgcgctaga cagcttattt ctcgctatgc taaggtgacg ttccttggtt gcgggagcgc
     1081 ctgtagctca gctggataga gcatcagcct ccggagctga gggccacagg ttcaaatcct
     1141 gtcaggcgca ccaaaaacac ataccaacac caggctgagg ttgaggaagc ccacgagccg
     1201 gttccctttc ttcaacctga acctcaagct ttcttgaacc cggtggggcc gttagctcag
     1261 ttggtagagc agctgactct taatcagcgg gccgtaggtt cgacccctac acggcccacc
     1321 aaacttttca aacacttaca cgcctgtgac accgctgaga cctggccggg ggggactaat
     1381 tctgtaccct ggcccgcgag ccgatcaaac aggtcgatcc catcgcgcaa gctttccgga
     1441 tagtgatgcg catagcgttg cgtcatgacg ggcgatttat gccccagcag atggagtgct
     1501 tcggagtgcg cgaacagttc ctgtgtcaga caacatggcc tctcagctca ctaccctctt
     1561 ccaagtcgct tcgtacgtct tcaactgcga gtcgtatgag gctggcatct actttcgatc
     1621 ggtgttccgc agccatgaaa agcagatcca atcgtttctt gcactggttg aggatatgca
     1681 ttgccagcga agcatcttct gcaaaccatt tcagcccgaa atttcgttga tgtgtcacat
     1741 cactgatctc gccaaggcat cgagtttgac cttgatccgc ttcggcacgc tgatcagcag
     1801 cttcaccatc gccataccat cacctccaat
//
LOCUS       DATMTI010000227         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_476214, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000227 DATMTI010000000
VERSION     DATMTI010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476214"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VN039_03840"
     CDS             <1..218
                     /locus_tag="VN039_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45147.1"
                     /translation="TIRTEQEKYAHNHIVDSDCVNAFCGQAVPAAVIECGVTDLRNPY
                     VGKGRVTCGDCLSELKNGRRNGYSQTT"
     gene            475..720
                     /locus_tag="VN039_03845"
     CDS             475..720
                     /locus_tag="VN039_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45148.1"
                     /translation="MSEQIGINVVKGCEHVTYTDHNGKPVTNVISRLYRYGVDDVTKQ
                     YVVAIEVRVGSFAIITTDSGKHYIPWFNVIEWFRRAI"
     gene            720..935
                     /locus_tag="VN039_03850"
     CDS             720..935
                     /locus_tag="VN039_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45149.1"
                     /translation="MDITPVWQYGETPVPQEILDTRCLVDPSMEDHIIALEQCECGAM
                     RVIWRQDYAQYGDPWKNHVNDCPNPTG"
     gene            948..1157
                     /locus_tag="VN039_03855"
     CDS             948..1157
                     /locus_tag="VN039_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45150.1"
                     /translation="MISLVWRIGDTPVPVEILETRAMVSPSMETDIVGLEKCVCGEVR
                     VMWENDPRDRWKHHFDYCPAYDENK"
     gene            1294..1539
                     /locus_tag="VN039_03860"
     CDS             1294..1539
                     /locus_tag="VN039_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45151.1"
                     /translation="MEKTFDNVIEIKLEIPDNDSYDRHVAVIERFGTYEGRHVDAIEV
                     GIVSDRMPYITLYHSKMRQTTVFMDVHIISFRTSEHG"
     gene            1532..1729
                     /locus_tag="VN039_03865"
     CDS             1532..1729
                     /locus_tag="VN039_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45152.1"
                     /translation="MADPLHDGSYWSATITLDNGSWRTCIGWGTDQLDALKSLIEEGY
                     IPSNVARITIKPHLYVRKWGK"
     gene            1726..1911
                     /locus_tag="VN039_03870"
     CDS             1726..1911
                     /locus_tag="VN039_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45153.1"
                     /translation="MKVFRLYFKPHNRSQQTGLEVFIRASNESAAVSRALTETRLTEF
                     AAIMVDVIDNAIIIDEE"
     gene            2620..2769
                     /locus_tag="VN039_03875"
     CDS             2620..2769
                     /locus_tag="VN039_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45154.1"
                     /translation="MSIDEIIELLREARYSIYESDSVSGDIAHECDVDITSAIVRLLS
                     ISDLS"
BASE COUNT          744 a          753 c          790 g          578 t
ORIGIN      
        1 tcaccatacg caccgaacaa gaaaagtacg cccacaacca catcgttgac tccgactgcg
       61 ttaacgcatt ctgcggtcag gctgtacccg cagcagtcat cgaatgtggc gtgactgacc
      121 tgcgtaaccc gtatgtcggc aagggcagag tcacctgcgg cgactgtctt tctgagctca
      181 agaatgggcg gagaaatggg tactcacaga cgacctagaa ccagcacccg aagatttccc
      241 atgtccaacg tggtccgccg atccgacgga gtatgggtgg gacgctgtaa ctgttgcggg
      301 acagcgataa cacgctattt ctggcgcaac gcgtacgacg acacccacaa ccacgtgaaa
      361 ttccgccaca cgtacatgat ggaagactca gattaatgtg ggaaatcatg tgcgcaacgg
      421 cggtagttgg tatactgctc atagcaatcg cagtcgacag cgggagaaac agcgatgagt
      481 gaacagatcg gcatcaacgt cgtcaaaggg tgtgaacacg tcacctatac cgaccacaac
      541 ggcaagcctg tcacaaacgt tatcagccgt ctctaccggt atggcgtcga cgacgtcaca
      601 aagcagtacg ttgtcgccat tgaggtgcgc gtagggtcgt tcgccatcat cacaacggac
      661 tcgggaaagc actacattcc ctggttcaac gtcatcgaat ggttcaggag ggcgatctag
      721 tggacatcac gcctgtatgg cagtacggag agactcccgt gccgcaagag atcctcgaca
      781 cacggtgtct cgtggatccc tctatggagg atcacatcat cgcgcttgaa cagtgcgagt
      841 gtggcgctat gcgcgtcatc tggcgacagg actacgcgca gtacggcgat ccgtggaaga
      901 accacgtcaa cgactgcccc aatccgacag gatgaatgga ggacggtttg atttctctag
      961 tttggcgcat cggtgacacc ccggtgcccg tcgaaatctt ggagacgcgc gccatggtaa
     1021 gcccttcaat ggaaacggac attgtcgggt tggaaaagtg cgtctgcggt gaagtcaggg
     1081 tcatgtggga gaacgaccca cgggaccgct ggaagcatca cttcgactac tgtcccgcgt
     1141 acgacgagaa caagtgaaat ccaggtactt cctatgtgac gcgaccggcc ggtatcgcgg
     1201 cctcttccgc attgaagagg aacatgccga ccggttgcgc cgcaaaggct ggaaaaccat
     1261 caaagcctca cccaggttcg gaaaggaaca cccgtggaaa agaccttcga caacgtcatc
     1321 gaaatcaagc tagagattcc cgacaatgac tcctacgacc gacatgtagc ggtcatcgag
     1381 cgattcggta cctacgaggg tcgtcatgtc gacgccatcg aagtcggcat cgtctctgac
     1441 aggatgccgt acatcaccct gtaccacagc aagatgcgtc agacgaccgt gttcatggat
     1501 gtccacatca tcagtttccg tacgtcagag catggctgac ccgcttcacg atgggtcata
     1561 ctggtccgcc acgatcactc tcgacaacgg gtcgtggcgg acctgtatcg gctggggaac
     1621 cgaccaactt gacgcactca agtcgctcat cgaagaaggc tacattccct ccaacgtagc
     1681 ccgcatcacc atcaagcctc atctgtacgt cagaaagtgg ggaaagtgaa ggtattcagg
     1741 ctgtatttca agcctcacaa cagaagccag caaaccgggt tggaagtgtt cattcgcgca
     1801 tccaacgaaa gcgcggcagt atcccgcgca ctcaccgaaa ccagactcac cgaatttgcc
     1861 gccatcatgg tcgacgtgat agacaacgca atcatcatcg acgaggagta acaggtgtca
     1921 cggaaattcc actgtgacga ctgcgatgaa atcatcaatt ttattcgcgt cgacactgac
     1981 ggtgaagacc ttcacgtaag gtgtctaatc gccagataca gtgattcgac cagaacattc
     2041 gtactcgacc caaatcccat cccgacggag cttctggccg aaatcgaatt cggcaaagag
     2101 gagtagcaaa tggtgaaaag gaagaacgac atcagtgaga gcgagttctc gcaattctca
     2161 agtcgcaaat cgagagcggc gaaattgctt tcggtgaaca attgccgacc agcaaggaat
     2221 tgatcaaatt gtacggtgct agcgcgtggt gctcctacga aatccggcag gtactcaagc
     2281 gagaaggcta catcgccgac catcactacg ccgacggcaa gcacgtgaca cttgtcatct
     2341 gggagcccaa gtgagacgct acagggtcct tctcacgcgc gataacaaga cgtggcggtc
     2401 acacgtcata accgtcgacg ggctcaagaa ggcaaccgac tggggttgca aagaagtaca
     2461 gagactcaag gacacgcgtg acgagaactg gttcgtcacc agtgtcacgc cactcgaaaa
     2521 gagaggtcca cggtaaacgc gactagaatt gcttgatttg cggcgtcact tgtgagatgg
     2581 gattgcaggt ggcgccgctt ttcttgggag ggagttgtta tgtcgatcga tgaaatcatc
     2641 gaattgcttc gcgaggcgcg ttactcgatt tacgagtctg acagtgtttc cggtgatatc
     2701 gcgcatgagt gtgacgtcga tatcacatca gctattgtgc ggcttctttc aattagcgac
     2761 ttgagttgaa tggggagttg tgggacatgt gggaattcgg ggagttgaat ccgagtggga
     2821 atagtcgcaa tgtaattcaa gtcgcgaaag tgggaatgtg ggaat
//
LOCUS       DATMTI010000228         1881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_222075, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000228 DATMTI010000000
VERSION     DATMTI010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222075"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1329
                     /gene="ftsW"
                     /locus_tag="VN039_03880"
     CDS             124..1329
                     /gene="ftsW"
                     /locus_tag="VN039_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWV45155.1"
                     /translation="MPQRSAGTLALPWSTVSARASKRVGMDHILLFIVISLALAGLVM
                     IFSASAVVAGQKHHDSWYYLKRQLAWLVLGLTLLHVVSRVDYIWWKRLALPLLGLIIV
                     LLVVVLIPSIGVAANGARRWLWLGFISIQPAEMAKLIAVIYLAAYLAKKEARLQHFST
                     GLLPALLIIGVISGLVLMEPDLGTIVVIGLVTAGMVFLGGARVTHLLSLVPVALVGVA
                     VLIWVSPYRWQRLIAFFYPEQDPTGTGFQINQSFLAFGSGGLFGVGLGESKQKLFFLP
                     EAHTDFVLALVGEELGFVGAGIIVLSFALFAIRGFQVSTRARISFGRYLGIGITTLIG
                     IQALINACVVTGLLPTKGLTLPFVSYGGSSLVINLIGVGILLNISRDRQAGREETRQQ
                     SGRGWASRS"
     gene            1326..>1881
                     /locus_tag="VN039_03885"
     CDS             1326..>1881
                     /locus_tag="VN039_03885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV45156.1"
                     /translation="MTIVIAAGGTGGHLYPAVALAREFLRRDPSTSILFVGTVRGVES
                     RVLAHEGFELALITAKPVMGKGLLDVVKGVLSVPIGIRQAPSILKRRRTDLVIGVGGY
                     TSPTMLVAAALKGIARVILEPNAHPGLANKVVASFAQRIFLAFESAGAFFDRRKVRIV
                     GTPIRQEFLVQPTDNVSTKQEGRHV"
BASE COUNT          354 a          465 c          586 g          476 t
ORIGIN      
        1 gccgcagcaa acgaagaggc gaagcgtact ttgtgctgta cggtgagcct ctaagtgatg
       61 cgaaaacgcc gctggcggac tttttcaaca gcctgccagg cagaggagca ttgacgctgg
      121 aagatgccac agagatctgc aggaaccttg gctttgccct ggtccacggt cagcgcgcgc
      181 gcttcgaagc gggtggggat ggatcatatc ttgctgttta tagtcatttc gctggcgctg
      241 gccggccttg tgatgatctt cagcgcaagt gccgtggtgg ccggtcagaa gcaccacgat
      301 tcttggtact atctgaagcg gcaactggca tggttggtgc ttggtttgac gcttctgcat
      361 gttgtgtccc gggtcgatta tatatggtgg aaacggcttg cacttcctct cctcggtctc
      421 atcatcgtgt tgctggtagt tgtccttatt ccatcgatcg gtgtcgccgc caatggggcc
      481 cggcgttggt tatggttggg gtttatttcg attcagcctg ccgaaatggc gaagttgatc
      541 gcggtgatct atcttgctgc gtacctcgca aaaaaagaag ctcgacttca gcatttctca
      601 acagggctgt tgccggcgct gctcattatt ggggtcatca gtgggttggt tctcatggaa
      661 ccagatttgg ggaccattgt ggtgatcgga ttggtgacgg ctggcatggt gttcctcggt
      721 ggagcacgag taacccatct cctcagtctc gtgccggttg cgttagttgg ggtggcggtg
      781 ttgatctggg tatcacctta tcgatggcaa cgactcatcg cgtttttcta tccggagcag
      841 gatccgaccg gaacggggtt ccagatcaac cagtcgttcc tcgcattcgg cagcgggggg
      901 ctcttcgggg tcgggttggg ggaaagcaag caaaaactgt tctttcttcc ggaagcccat
      961 accgattttg tgttggcgct cgtcggcgaa gagcttgggt tcgttggagc ggggatcatc
     1021 gttctttcct tcgcgctctt cgcgattcga ggatttcagg tctccaccag ggcgcgaatc
     1081 tcgtttggcc gctacctggg gatcggcatc acgacgctca tcggtatcca ggcactgatc
     1141 aatgcctgtg ttgtgaccgg gttgttgccg accaaaggtc tcacattgcc gttcgtcagt
     1201 tatggcggat cttccttggt gatcaacctg atcggtgtag ggattttgct gaacatctcg
     1261 cgggatcggc aggccggacg agaagagacg agacagcaga gcggacgtgg atgggcaagt
     1321 cgatcatgac gattgtaatt gccgctggtg ggaccggtgg gcatctctat ccggctgtcg
     1381 cgctggctcg ggaatttctg cgccgcgatc cttccacgag cattctcttt gtggggacgg
     1441 tgcgcggcgt cgagtccaga gtgctcgctc atgaaggatt cgaattggcg ttgatcaccg
     1501 cgaagccggt catgggaaag ggactgctgg atgtcgtaaa gggagtactt tctgtaccta
     1561 ttgggatccg gcaagcacca tccatcctga agcggcggcg gactgattta gtaatcggag
     1621 taggtggcta tacgagtccg accatgctgg tagctgccgc cttgaaagga attgcccgcg
     1681 tgattctgga gccaaatgcc catcccggat tggccaacaa agtcgtcgca tcatttgcgc
     1741 agaggatttt tttggcattt gagtcagccg gggctttctt tgaccgacga aaggttcgca
     1801 tcgtcgggac accgatccga caagagttct tggtacaacc aaccgacaac gtgtcaacca
     1861 agcaggaagg ccggcacgtg c
//
LOCUS       DATMTI010000229         1693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_222085, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000229 DATMTI010000000
VERSION     DATMTI010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222085"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..508
                     /locus_tag="VN039_03890"
     CDS             368..508
                     /locus_tag="VN039_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45157.1"
                     /translation="MLTDERAELLHWLRTLSDGLSIATKLERDLTDADDSDFALSRTG
                     CH"
     gene            complement(761..1168)
                     /locus_tag="VN039_03895"
     CDS             complement(761..1168)
                     /locus_tag="VN039_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWV45158.1"
                     /translation="MSWRDWSDERRGFEWWHLELIVTDRCSGLTTALQTVCPIASHQH
                     CWAHKPRNLLNDTERRGHVAMKAATQSIYQATSLTEVGHVDIIFARRWFEVYSMFVAR
                     LLQGLSEFFAFLQCQRTLWGRLRTTECADGHDP"
     gene            complement(1348..>1693)
                     /locus_tag="VN039_03900"
     CDS             complement(1348..>1693)
                     /locus_tag="VN039_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45159.1"
                     /translation="IASFVAPKGRQSAGKSWNAAVSMVAQHVNATHKQTTSQILITPS
                     FGRGCRAIKKWPHSTLRVCAKSCPRLVSRHQPTGTDRSTPAVAMADQWQRLQMELGEQ
                     FWRDLTPQMRQS"
BASE COUNT          381 a          473 c          466 g          373 t
ORIGIN      
        1 ggctgtgaac ggcgctggct tcggtgctcg tgtcgtacgc gaagacctcg aacaatcagc
       61 tcatggccat agacgtaccg cagcgcagac gcggtggtca atatgtatgt ctattagcgc
      121 accaatagtg agaagctaat caaggaacca aggatagtct gggcctggac acattctgtc
      181 tccagacgct cgacgaaacc gatgtcacat ttctgggccg gcagcgccct cgacaacctt
      241 cttctgggct ttcgagagac ggtgctcccc tcgtgctggc ttaaacgacg actacgaacg
      301 atatgcgatt gggttttccc gatctgagcc gattcaagaa gctcgacggc tcaggatgtg
      361 gttcgccatg ctcacggatg agcgggccga gcttctccac tggttgcgga cgctttcaga
      421 tgggttgtca attgcgacga agttggagcg ggacctgacc gatgccgacg attcggattt
      481 tgctctctca cgcaccggat gtcactgacg actgagtagt cgaatccgtc taggccgccc
      541 gactcatacc aactatagcc agatcatgga gacgcctcat gatcgaggac gacatcaata
      601 atttgttgga catgtccaac aaaattttgc ctcctcatga tgcgagcatt tagttcgatc
      661 ggaaaggaat agtacgggtg agaccgacca tcgattactg gaggtagatc ctcagacttc
      721 acgataatcc gttccacgct ctgcacgttc acgaaacaga tcatggatcg tgtccgtcgg
      781 cgcactccgt ggtccgcagt ctgccccaga gtgtgcgttg acactgcaag aacgcgaaaa
      841 actctgagag accctggagc agcctggcca cgaacatcga gtacacttcg aaccagcggc
      901 gagcgaagat gatgtcgaca tgccccactt cggtcaggct cgtcgcctga taaatggatt
      961 gggtagccgc cttcatcgcg acgtgacccc gtcgttcggt gtcattcaac agattacgag
     1021 gcttgtgcgc ccaacaatgc tgatgcgaag cgatcggaca gaccgtctgg agcgccgtgg
     1081 tcaagcctga acacctatcg gtcacgatca actcgagatg ccaccattca aaccctcgtc
     1141 gctcgtcgct ccaatcgcgc cagctcaatg gtgagaaatg cctgctgacg ggtgcgggcc
     1201 atcctgattt tcaagatgtc gattctggtc atgagttatg ctcgtaaaag ccgctcctga
     1261 catcggtcat gcaggactgc gatcatactc tctcacgccc agacaacgat ttctggattc
     1321 gattgagagc cgaccaaaaa agccttatca actctgtcgc atctgcggag tcagatcgcg
     1381 ccagaactgc tccccgagct ccatctgcaa acgttgccat tggtcggcca tcgcgacagc
     1441 cggagtcgag cgatccgtgc cggtaggctg atgcctagaa accagcctgg gacatgattt
     1501 tgcgcagacg cgcagggtgg aatgcggcca tttcttgatg gctctgcacc ctctgccgaa
     1561 ggagggcgtt atgagaatct ggctggtcgt ctgcttgtgt gttgcattga catgctgcgc
     1621 aaccatggag accgctgcat tccaagattt cccggctgat tggcggccct taggggcgac
     1681 gaaagaggcg atc
//
LOCUS       DATMTI010000230         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_179322, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000230 DATMTI010000000
VERSION     DATMTI010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179322"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..2233)
                     /locus_tag="VN039_03905"
     CDS             complement(206..2233)
                     /locus_tag="VN039_03905"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWV45160.1"
                     /translation="MEREVKTYVLKRPTEEAGPRKLSINYAAALNPQQLAAVTAAEGP
                     SLVIAGAGSGKTRTLVYRVAYLIDSGVDPSNILLLTFTRKSAQEMLERAGDLIGTSSH
                     RVCGGTFHSVANLLLRRYGRSIGIEPGFTILDRGDAEDLIALVRSQLGLNEKDKRFPR
                     KGTIMEMISKSENTLCSLDEIILEEFSHFADHSEDLGRLQKAYQSAKRQKQLLDYDDL
                     LVMLRQLLLVDEVARANISRQYRYILVDEYQDTNRLQAEVIRQLATTHQNVMVVGDDS
                     QSIYAFRGATFKNIMDFPVLFPGTTVYKLEENYRSTQPILNLANCIIEEATEKYTKRL
                     FTRKIDGPLPALVEAAGENAQSRFIAQKILELREEGVPLDEMAVLFRSSFHSFDLEIE
                     LSRKGLPFIKRGGVKFIETAHVKDLLAHVRVVSNPLDTVSWHRVLMLVEGVGPKKAQD
                     LLAAIVKAEQPFDVLRGVSGRSGQGLKNLADTLESLAGPDDRQPAEQVNHIYEYYLPI
                     LKDQYDDYPKRTRDLDHLHTIAEGYHGIDEFLADLALEPPDGSAVGVEAFDRDDERLV
                     LSTIHSAKGLEWQCVFVIWVVDGRFPSAYSFLTDDELEEERRLFYVAVTRAKRHLFLT
                     YPINVYDKTSGMLLSKPSRFLDHVSSDLLDTLALVEEGGREDWGMARDPYY"
     gene            complement(2309..2851)
                     /locus_tag="VN039_03910"
     CDS             complement(2309..2851)
                     /locus_tag="VN039_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HWV45161.1"
                     /translation="MDAFHLPPMASHRQSGSPIEVLPRDEAREHLLAIADMLAKVLDT
                     TVRIPGTSWYIGLDPLLGLIPGIGDVIANLIGTIILGLATRLQLPRIVLVRVSLNLLI
                     NGAVGAVPIVGDLFSVWFRSHTRNAVLLREAAMKPDRETHADWFYVVGIIGGTASLLL
                     LTIAFVVWLVFKIWTMVAGG"
BASE COUNT          798 a          967 c          766 g          688 t
ORIGIN      
        1 cttcgtcggc agctgcacgc gccctgcgtc atcgagtagg cgcgcccata tctggctttt
       61 accactctct ccattccgag ctgtgacagc ggcttccgca aggaaggcca gtaccaacca
      121 gcagcaccat gccagacaca atcgaatcgt gatgtgattc atacaggcag gaagaggact
      181 gagcatgtaa caacaaggaa ccgagttaat aataggggtc tcgtgccatc ccccaatctt
      241 ctcgcccacc ttcttctacc aacgcaagcg tatcgagcaa atccgaggac acatggtcca
      301 gaaaccgtga gggctttgaa agcaacatcc cgctggtctt gtcgtacaca ttgatgggat
      361 atgtcaaaaa caaatgccgc ttggctcgag tgacggccac atagaaaaga cgccgctcct
      421 cttccaattc atcgtcggtg agaaaggaat aggcggatgg aaaccggccg tcgaccaccc
      481 agatgacaaa cacgcactgc cactccaacc ccttcgcgga atggatcgtc gaaagcacaa
      541 ggcgttcatc atcacggtca aacgcttcga cccccacggc actcccatca ggcggctcca
      601 gcgccaagtc ggcgagaaat tcatcgatgc cgtgataccc ctcggcaatc gtgtggagat
      661 gatcgagatc tctcgtgcgc ttcggatagt cgtcgtactg atccttgagg atcggaaggt
      721 agtactcata gatatgattg acctgttcgg caggctgccg atcatccggt ccagccagac
      781 tctccagtgt atcggccaag ttcttgagcc cctgcccgga acggccgctc accccgcgca
      841 gcacgtcgaa tggttgttca gctttcacga tcgccgcgag caaatcttga gctttcttcg
      901 gccccacgcc ttccaccagc atcaacacgc gatgccagct gactgtatca aggggattag
      961 agacgacccg cacatgtgcc agcagatcct tgacgtgggc cgtctcgata aacttcaccc
     1021 cgccgcgctt aataaaaggc aaccccttgc gcgagagctc gatttccaag tcaaatgaat
     1081 ggaaactgga ccgaaacagc acggccatct catccagcgg cacaccttct tcacgaagct
     1141 caagaatctt ctgggcaatg aatcgggatt gtgcattttc accagcagct tccaccaacg
     1201 ccggcagagg cccatcgatc tttctcgtga agagccgctt cgtatacttc tcggttgctt
     1261 cttcgataat gcagttagcg agattcaaaa tcggctgagt gctgcggtag ttttcttcga
     1321 gcttataaac cgtcgtgccg gggaacagca cggggaagtc catgatgttc ttaaaggtcg
     1381 cgccacggaa cgcatagatg gactgcgaat cgtctcccac caccattaca ttctgatggg
     1441 tcgttgccag ctggcggata acttcggcct gcaatcgatt cgtatcttga tactcatcga
     1501 ccaggatgta gcgatactgc cgggagatgt tcgcgcgggc cacttcgtca accaacagca
     1561 actgccgcag catgaccagc agatcgtcgt agtccaagag ttgcttttga cgcttcgccg
     1621 attgataggc tttttggagc cgtccgaggt cctccgaatg atccgcaaaa tggctgaact
     1681 cttccaggat gatctcatcc agactacaca gcgtattctc gcttttgctg atcatttcca
     1741 tgatcgtccc cttgcggggg aagcgcttat ctttttcgtt cagacccagc tgcgaccgta
     1801 caagcgcgat gagatcctcc gcatcgccac gatccagaat ggtgaatcca ggttcgatac
     1861 cgatcgaacg gccataccga cgcagtaaca gattcgccac ggagtggaac gtccctccac
     1921 aaacccgatg actactggtc ccgatcagat cacctgcacg ctccaacatt tcctgcgcgg
     1981 acttgcgcgt gaacgtgagc aaaagaatat tcgaaggatc cacgccggag tcgatcagat
     2041 aggctacgcg atagaccaac gtgcgcgtct taccgcttcc agctcccgca atgaccagcg
     2101 aaggcccctc agccgccgtc accgccgcca gttgctgagg attcagcgcc gccgcatagt
     2161 tgatggacaa cttgcgcgga cctgcctcct cagtgggccg cttcaatacg taggttttga
     2221 cttctcgttc catggaatat gaaaaggagt atccgaaagt atcgttaatc gggaaggttt
     2281 gttctatatc acattcgact tccctttcct atccacccgc gaccatagtc cagatcttaa
     2341 agaccaacca gacgacgaag gctattgtga gcagcaggag cgatgcggtc cctccgatga
     2401 ttccgaccac gtagaaccaa tcggcatggg tctcgcgatc cggcttcatg gccgcctcac
     2461 gaagcagcac ggcatttctc gtatgactcc gaaaccagac cgaaaagaga tcaccgacga
     2521 tcggaactgc gccaaccgct ccattgatca gcaaattgag actcaccctg accaacacaa
     2581 tccggggaag ctgtaaccgc gtcgccaacc caagaataat ggtgccgatc agattcgcaa
     2641 tgacatcgcc gatcccaggg atgagaccaa gcaagggatc caacccgatg taccatgacg
     2701 tgccgggaat cctgaccgtc gtatccagca ccttggccag catgtccgcg atcgcgagca
     2761 gatgctcacg cgcctcatcg cgcggcaaga cctcgatcgg tgatccagac tgacgatgag
     2821 aagccatcgg aggcaaatgg aatgcgtcca tctgattatc gccattctaa tgtgatctct
     2881 accgaagaac gcaacaacgt tttcgtcaaa tagcgattta ccccaatcac atcactgtga
     2941 catgttgcgc acaacttgac ccataaatag ctaatatgta caatttagct atatgagtta
     3001 ttaattcctt gccgaaacat catgcccaag ctcacattca gaaacagcca tggacagctc
     3061 ataggcatct cctccaaacc aatcgtcgcc gcagcgctca agctacacca cgcataagtc
     3121 cttccgacct gagtttcctt acacactcgc cacattcacg cacacgtggg agactcccgg
     3181 ctccatatcc cgcactgagc taaattccat gtttttgat
//
LOCUS       DATMTI010000231         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_51253, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000231 DATMTI010000000
VERSION     DATMTI010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51253"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..497
                     /locus_tag="VN039_03915"
     CDS             126..497
                     /locus_tag="VN039_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWV45162.1"
                     /translation="MTPKLSPQHSALVMSGIVEDVSDANYKEFTDSAGAVVAYGLATC
                     DPCKAYDPILEATAAKFPEIKIGKAKMHVPGRCREIKKTHTFETYPTTHFFAHGKLLF
                     TREGVVEPAELAALISDHLLK"
     gene            599..1078
                     /locus_tag="VN039_03920"
     CDS             599..1078
                     /locus_tag="VN039_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45163.1"
                     /translation="MNNTIAALVFCVTLALSIPAHAHTDEFFESVEAPHGGQLRMTGP
                     FHMELVAKGGDLTVYVTDHADNAISVDGGLAKANIENGSTRTQVNLHPVGSNVLRGSG
                     TFSLTSNTVVVIFMKLPNQDGYAARFMPLKPKGERGEKAASHADDAGPHHHHHPPKH"
     gene            1094..1549
                     /locus_tag="VN039_03925"
     CDS             1094..1549
                     /locus_tag="VN039_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45164.1"
                     /translation="MKHLLRNLALCTTLLAAVPVGAHTAKQADPVKALHGGQSLAAGP
                     YHLELVAKDGELVLYVTDHSDKAIPTDGAKAKATIQQGYEKAKIQVELEPSGENSLKG
                     TGTFMINSDTGIIVFLRLPEQEAYAARFTPLNTKGGAAQRGEHQHPPRH"
     gene            complement(1622..1960)
                     /locus_tag="VN039_03930"
     CDS             complement(1622..1960)
                     /locus_tag="VN039_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45165.1"
                     /translation="MLMEARRTLLARMEIPTEQQDKQMRGRLPVNDLHQCSCHVLHLS
                     LRHLLYGDDSEETRQLAERVTEIKTKFWIGNDEQDALYRAADVIMKEVDAQHLLSDES
                     LKPQCAASKQ"
     gene            complement(1954..2211)
                     /locus_tag="VN039_03935"
     CDS             complement(1954..2211)
                     /locus_tag="VN039_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45166.1"
                     /translation="MDRPHYVHLYDGGPIDNLGIQAVVEYLNRNILGTTLDRLFPKGC
                     LIIAIDATPASELRTSMPEFPVGNSRTISSTRTRLMRRMPC"
     gene            complement(2226..2792)
                     /locus_tag="VN039_03940"
     CDS             complement(2226..2792)
                     /locus_tag="VN039_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWV45167.1"
                     /translation="METAGSWKLTEAETQDGRFLALAVSGGGSRAANFGAAVMLELQQ
                     RGLLEQADVISGVSGGTLPAVYYGLGAKAGRFTAPALREALGYDFQSSWIRRWFLPHN
                     IFRYWLADFTRSDIMVQVFNNQLYREATFADLKLHPKILLNATVHNDHTRFTFTDDRF
                     ALLRSGLAQYRVANAVNASSLFPAPFRM"
     gene            3080..>3752
                     /locus_tag="VN039_03945"
     CDS             3080..>3752
                     /locus_tag="VN039_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV45168.1"
                     /translation="MPGIALVTGANRGIGLEVVRQLAAKQWRVFLSGRSLDSIERAAV
                     SLSAPSVTPIVLDVTDQESIDRAVKTIAQTVDHLDVLVNNAGILDDHDDSVFKIPADR
                     LRRMFETNTIGPLLVTQAFVPLLRKSAAARIINVSSRVGRLTGMGSAVPAYGISKTAL
                     NGVTCKFAASLKSSGIAVNSVDPGWVRTEIGGSDAPRSVEQGTDTIVWLATEAPHSMA
                     GLFLRD"
BASE COUNT          887 a         1085 c         1053 g          727 t
ORIGIN      
        1 aacggcaaag tcgcctacgc gaaggtgcag gaaatcaaga cggcacgaga ggacaaggag
       61 atcctcgatg cgctgtcgaa gctgaactaa cgatacagga ctgagcaacg aggactgagg
      121 actgggtgac tccgaagctc agtcctcagc actcagcact cgttatgagc ggaatcgtcg
      181 aggacgtcag cgacgccaac tacaaagagt tcaccgacag cgccggtgcg gtcgtcgcgt
      241 acggccttgc cacctgcgat ccctgcaagg cctacgatcc cattcttgaa gcgaccgctg
      301 cgaaattccc cgagatcaaa atcggcaaag ccaagatgca tgtgccgggc cgctgccgtg
      361 agatcaagaa aacccacacg tttgaaacat accccaccac tcatttcttc gcgcatggta
      421 aattactgtt cacgcgtgaa ggagtcgtcg agccggccga actcgccgca ctcatttcgg
      481 accacctgct caagtagcgt ccatgttcat tgagtagttg aatcgagaga agcctttcca
      541 ccgcagccgg atcaccggaa gcggtgtaat gatcggcgag ttaggagaca acctgagcat
      601 gaacaacacg attgcggctc tcgtattctg tgtgaccctg gccctttcaa tcccggctca
      661 tgcccatacc gatgaatttt tcgaatccgt tgaagctccg cacggcgggc aattgcgcat
      721 gaccggtccg tttcatatgg aactggtcgc caaggggggt gacctcactg tgtacgtcac
      781 ggatcacgcg gacaacgcca tcagcgtgga cggcggctta gccaaggcga acatcgagaa
      841 cgggtcaaca aggacacagg tgaacttgca cccggtaggg agcaatgtcc ttaggggctc
      901 cggcaccttt tcgctgacct caaataccgt cgtcgtcatt ttcatgaagc tgccgaatca
      961 agacggctat gccgcccgct tcatgccgtt gaaacccaaa ggcgaacgag gagagaaagc
     1021 tgcatcccac gcggatgatg ccggccccca ccaccatcac catccgccga agcattgagg
     1081 gggagaaccg atcatgaaac atctgctgag gaacttagct ctgtgcacaa cactactcgc
     1141 ggcggttccg gttggagccc acacggcgaa acaggccgac cctgttaaag ccctccacgg
     1201 cgggcaatcg cttgctgcgg gaccgtatca cctagagttg gtcgccaagg acggagaact
     1261 ggtgctgtat gtaacggatc attccgacaa ggccatcccc actgatggag cgaaagccaa
     1321 ggccaccatc cagcaagggt atgagaaggc caaaatccag gtggagttgg aaccgtccgg
     1381 tgaaaacagc ctcaagggca ccgggacatt tatgatcaac tccgataccg ggatcattgt
     1441 cttcctcaga ttaccggaac aggaagccta cgcagcccgt ttcactccgc tcaacaccaa
     1501 gggcggagcc gcgcaaagag gcgagcacca gcatccgcca aggcattgat tactgctctg
     1561 ctgatctcga cagaggatca agaggagtga gagggaaaag ccgaaagata agatctcttg
     1621 gtcactgttt gctcgccgca cattggggct tcagtgattc gtctgaaagg agatgctgcg
     1681 cgtccacttc cttcatgatg acatcggccg ctcggtagag cgcgtcctgc tcgtcattcc
     1741 cgatccaaaa cttcgtcttg atctcggtca ctcgctcggc caattgtcta gtctcctccg
     1801 aatcgtcgcc gtagagcaga tgccgaagag agagatgaag cacgtgacag ctgcattgat
     1861 gcaaatcgtt taccggcagc cgaccccgca tctgcttatc ttgctgttca gtcggaattt
     1921 ccatacgcgc gaggagagtt ctgcgcgctt ccatcaacat ggcatccgtc gcatcaagcg
     1981 cgttcgtgtc gatgaaatag tccgcgaatt gccgactgga aattctggca ttgaggtccg
     2041 gagctcgctt gcgggcgtcg cgtcgatcgc gataatcagg cagcctttgg gaaagagacg
     2101 atcgagcgtc gtccccagga tgttgcggtt caaatactcg acgacggcct gaatgcccag
     2161 attgtcgatg ggaccgccgt cgtagagatg cacgtaatgc ggccggtcca tgtaccactg
     2221 aagcgtcaca tcctgaaagg cgccgggaaa agcgaagagg cgttgaccgc attcgccacc
     2281 cggtattgcg ccagaccgga acgcaacaac gcaaagcgat cgtcggtaaa ggtgaagcga
     2341 gtatgatcat tgtgaacggt cgcgttgagc aggatcttcg ggtgaagctt cagatcggcg
     2401 aaggtcgcct cgcggtacag ttggttgttg aacacctgaa ccatgatgtc ggaacgggtg
     2461 aagtcggcca accaataacg gaagatgttg tgcggcagaa accagcggcg gatccaactg
     2521 ctctgaaagt catagccgag cgcctcgcgc aaggccgggg cagtgaagcg accggccttg
     2581 gcacccaacc cataatagac ggcaggcaac gtgccgcctg agacaccgct gatgacatcc
     2641 gcttgttcca gcagaccacg ttgttggagc tccagcatca ccgccgcgcc gaaattagcc
     2701 gcccggctgc cgcctccgga aactgcaagg gccaggaagc gaccatcttg cgtttcagcc
     2761 tcggtcagct tccaagatcc ggccgtctcc atgaccggct ttccgtcgtc ccaaggctcg
     2821 ttccatcccg gtcgccagtt gcccggcaac atctgacaag ccgcgagggc cgtccagaga
     2881 caaattatgg tggataacag tcgacctaac cgcatatcag gtccaatggc cggctagaac
     2941 gatcatgagc atgagtaatt tcagaggccg cgtgattgta ggacgccgca gaaagacatg
     3001 caaggcttga agaggacgga aagctacact ggtgcaggga attgatataa gtaagctatt
     3061 caataggtgg aggcgtcgca tgcccggcat tgctctcgtg accggcgcaa atagaggcat
     3121 cggccttgag gtagtccgac aactggcggc aaagcagtgg cgggtgttcc tgagcggacg
     3181 atccctcgat tctattgaac gagccgctgt ctccctctcc gctccgtccg tcactcccat
     3241 cgtcctcgat gttaccgatc aagaaagtat cgaccgggct gtgaagacca tcgcccagac
     3301 tgtcgatcat ctcgatgtcc tcgtgaacaa tgccgggatc ttggacgacc atgatgattc
     3361 ggtcttcaag atacctgccg atcggctccg gcgaatgttt gagaccaaca ccatcggtcc
     3421 cttgctcgtc acccaggctt ttgtccccct gctccgcaaa agtgccgccg ctcgaatcat
     3481 caacgtctcg agtagagtcg gacggttgac cggcatgggt tcagcagttc cagcctatgg
     3541 catttccaaa acggcgctca acggcgtgac ctgcaaattc gcggcatcgc tcaagagctc
     3601 gggcatagcg gtaaattccg tcgaccccgg ctgggtacgg actgagatag ggggaagcga
     3661 cgccccacgc tcagtcgaac agggtacgga taccatcgtc tggctggcta ccgaggcgcc
     3721 tcactcaatg gccggcctgt ttctccgaga tc
//
LOCUS       DATMTI010000232         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_222159, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000232 DATMTI010000000
VERSION     DATMTI010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222159"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VN039_03950"
     CDS             complement(<1..404)
                     /locus_tag="VN039_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45169.1"
                     /translation="MSHAYRHHQTSTYLAHLFSIILLIGLAACSAGDSSSPPVAAAPG
                     TPGAEEDVGERLFLDTRFAQTFKVFMDNGGNVNNPNVDDSVVATLETLGAPINPGPFK
                     GMSMNCRTCHLVDDALSSPAGGMRAYADFAHR"
     gene            complement(538..1230)
                     /locus_tag="VN039_03955"
     CDS             complement(538..1230)
                     /locus_tag="VN039_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWV45170.1"
                     /translation="MPIHIADQVEPTVLTIQNMLQKIDRLHLPATSRRDLESILVRQI
                     GKELDQLLPWQAGHGQETAAVAVRMGSATNLDDELLHQLKLAALLHDIGLLMLPPHLQ
                     TGREARDPESYVAIQNHPRLGANLLEPFSFLRDATIMIAHHHERWDGSGYPYGLRGEF
                     IPLGARILAVADAFEAIQVPHVRDRSLRDRIALRILRVASGTQFDPFVVDLLVELMGE
                     TETTYLQGTPHR"
     gene            complement(1336..1854)
                     /locus_tag="VN039_03960"
     CDS             complement(1336..1854)
                     /locus_tag="VN039_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45171.1"
                     /translation="MRNFNQKFRLRDADRHIRLLYTLFLLLMLAGFAFSFFWAHSMTS
                     LSPQGIADHYRGSDTTFGEPMSFRELAEITHFHLFTMPVVFMILIHVMYLTSASHMLK
                     TVVTWAGFGGVILDLLSPWLISYVSPVFVVSMLTGDTLMTVSFLVMLIVPLYEMWVLE
                     QPLMGGKRPQES"
     gene            complement(1879..>2295)
                     /locus_tag="VN039_03965"
     CDS             complement(1879..>2295)
                     /locus_tag="VN039_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWV45172.1"
                     /translation="PEESFEVYIGESGTQIDGYAVVQNTIGKHKPMTYMVGVDNEGSV
                     SNVELLVFRESRGSDVRKKRFNVQYEGKTVLDPIRIKRDITNITGATMSVRSMSAGVK
                     RVLVLIDEFYLKPAGIGSDTMAAQKDKGFFSSIFGN"
BASE COUNT          541 a          604 c          651 g          499 t
ORIGIN      
        1 ctgcggtgtg caaagtcggc ataggcccgc ataccaccgg cgggagagga gagggcatca
       61 tccaccagat ggcaggtccg acagttcatc gacatgccct tgaatggtcc cgggttgatc
      121 ggcgctccta aggtttcgag ggtagcgacg acgctgtcat cgacattggg attgttgacg
      181 ttgcccccat tgtccatgaa gaccttgaac gtctgcgcga atcgggtgtc aaggaacagc
      241 cgttcgccga cgtcctcttc ggccccaggc gtacccgggg cggcggcgac aggcggtgat
      301 gagctgtcac cggccgaaca ggcagccagg ccgatgagga gaatgatcga gaataaatgg
      361 gcgaggtatg tcgatgtttg atgatgccta taggcatgac tcatggctca ttattccttc
      421 tgttaaacgg acctccgggg ggaggtgagg gtcctcggga ggtgagggcc ctcgcctgca
      481 aagtggaaga ggtgagctcc cacttcacgg gccggcgtaa gtcgtacacc ggctgtgtca
      541 ccgatgaggt gttccttgta aataggtggt ttctgtttct cccattagtt cgaccaatag
      601 atcaacgaca aagggatcaa attgagttcc ggaggccacg cgcagaattc ggagcgcgat
      661 acgatcgcgc aaagatcggt cgcggacatg aggaacctga atagcctcga atgcgtcggc
      721 gacggcaaga attctggcgc ccagcggaat gaactctcct ctgagaccat agggatagcc
      781 tgatccgtcc catcgctcat gatgatgggc gatcatgatg gttgcgtcac ggagaaagga
      841 aaaaggctcg agtaaattgg ccccgagtcg cggatgattc tgaatggcca catacgactc
      901 cggatctcgg gcctctcgcc cggtttggag gtgtggggga agcatcagga gaccgatgtc
      961 gtgaagcaag gcggctagtt tcagttgatg caagagctcg tcatcgaggt tcgtggcaga
     1021 ccccatacga accgcaacag ccgccgtctc ttggccatgc cctgcttgcc acggcaggag
     1081 ctgatccagt tccttaccga tttgccggac caggatgctt tcgagatccc gccgcgatgt
     1141 cgctggaaga tgaaggcggt cgatcttctg cagcatgttt tgaatcgtca gcacggttgg
     1201 ttcaacctga tctgctatat ggattggcat cacatcacct ccgcaaaagc acagagccca
     1261 agaccattgg cgtgatctcg ggctctgggt gaataaccgc tggcctctca aacaattgtg
     1321 ccggcaatct gcatcttacg attcttgcgg tcgttttcct cccatcagcg gctgctcgag
     1381 cacccacatc tcatacagcg ggacgataag catcaccagg aaactgacgg tcatcagggt
     1441 atcaccggtc aacatcgaaa cgacgaaaac aggggagacg tagctgatca accatggcga
     1501 caggagatcg agaattacgc cgccgaagcc ggcccaggtc acgacggttt tcagcatatg
     1561 acttgcactg gtcagataca tcacatggat gagaatcatg aatacaacgg gcatcgtaaa
     1621 aagatggaag tgagtaattt ctgccaattc ccgaaaggac atgggctccc caaacgtcgt
     1681 atcggaccct cgatagtgat cggcaatgcc ttgcggggag agactcgtca tgctatgcgc
     1741 ccaaaagaaa gagaaggcaa agccggccaa catcaagagc aagaacaggg tatagagcaa
     1801 ccgaatatgt cggtcagcat cacgcagcct gaatttctga ttaaagtttc gcatgccggg
     1861 acgctgggtc cgcttgaact aattcccgaa tatggacgaa aagaatcctt tatctttttg
     1921 tgcagccatt gtgtcgctgc cgattccggc cggttttaag taaaactcgt cgatcagtac
     1981 caatactcgt ttgactccgg cgctcatcga gcgaacggac atcgtggcgc cggtgatgtt
     2041 ggtaatgtct cttttgatcc gtatcggatc gagaacggtc ttgccctcat actggacgtt
     2101 aaaccgcttc ttccgaacat cgctcccgcg cgattcgcgg aacacaagca gctcaacgtt
     2161 cgatacggaa ccttcgttat cgacgccgac catgtaagtc atcggcttgt gcttgccgat
     2221 cgtgttttgg acgaccgcat agccgtcaat ctgagttccg gattctccga tgtacacctc
     2281 gaatgattct tccgg
//
LOCUS       DATMTI010000233         1856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_518688, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000233 DATMTI010000000
VERSION     DATMTI010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518688"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /gene="larE"
                     /locus_tag="VN039_03970"
     CDS             complement(<1..562)
                     /gene="larE"
                     /locus_tag="VN039_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HWV45173.1"
                     /translation="MHPSLLQQKLDRLRTLLTEMGSVVVAYSGGIDSTFVLKVAHDQL
                     HEKAVGITAISPTFPSIELEAAERVAQEIGARHETVQTDQLAIDDFVRNDANRCFHCK
                     RDLYQLLGNVRQSKATTYVVDGTNLDDLGDDRPGIKAAREWGVRSPLVEAELSKADIR
                     ILAKELGLSNWDKPAAACLSSRIPRGT"
     gene            complement(570..>1856)
                     /locus_tag="VN039_03975"
     CDS             complement(570..>1856)
                     /locus_tag="VN039_03975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWV45174.1"
                     /translation="VARPGDAADISPARFTTPNGMTVLVLEQQHFLPIVEIHALIKAG
                     SAQDPPEKAGLANLVAGLLDEGTTTRSSKQLAEQIDFVGGSLGAQADEDFTTASARTL
                     KKDIDLGFTLLADILQRPAFPKQEFERVRSQILGEITSDNDDPGHVAMKAFNQLIFQG
                     HPYRWPVNGTEDTLGKITLADVQTFYAKEYLPNQVILTIVGDVTVEQATTLVQTHFGS
                     WKKGAVQPRTAKKPAVIDKKVVQLIEKDLTQSTIVIGHPGISRTNPDFYAVTVMNHVL
                     GAGGFSSRLMDTIRDKQGLAYGITSHYDARSMPGSFWINLQTRTETTNQAINGVLTEM
                     KTIREAPVSDQELADAKAFLMGSFPLRLDSTAKLAKVLAQVEFFGLGFDYFSQYPKWI
                     ERVTKEDVQRAAKQYLDPQHYALVVVGNIAKAKVRH"
BASE COUNT          395 a          499 c          519 g          443 t
ORIGIN      
        1 gggtcccacg cgggattctt gacgaaagac aggcagcggc cggcttatcc caattcgaaa
       61 gtccaagctc cttggcaaga atgcggatat cggctttcga cagttcagcc tcaacaagcg
      121 ggctgcgcac gccccattcc cgagcggctt tgatgcccgg acggtcatca cccaggtcgt
      181 ccaagttggt tccatctaca acatatgtgg tagcctttga ttgccgcaca tttccaagaa
      241 gttggtacag gtccctctta cagtgaaaac aacgatttgc gtcattcctc acgaagtcat
      301 caatggccag ctgatccgtc tgtacagttt catggcgggc gccgatctcc tgagcgaccc
      361 gttcggcagc ttccaactca attgatggaa acgtcgggga gatggccgtg atgccgaccg
      421 ccttctcgtg gagttggtcg tgcgcgactt taagaacgaa cgtgctgtcg attccaccgg
      481 aataggcaac cacgaccgag cccatctccg tcaggagcgt tcggagccta tcgagcttct
      541 gttggagaag ggacgggtgc atagtgtgtc tagtggcgaa ctttcgcctt cgcgatattg
      601 ccgaccacga ccagcgcata gtgctgaggg tccaggtact gcttggccgc gcgctgcaca
      661 tcctcttttg tcactcgttc aatccatttt gggtactgac tgaaatagtc gaatcctaac
      721 ccgaaaaact ccacttgtgc caaaactttc gccagcttcg ctgtagaatc cagccgcaag
      781 gggaagctgc ccatcaaaaa tgctttagca tcggccaatt cctgatcgct gaccggggct
      841 tctcgaatcg tcttcatttc agtcaagacg ccgttgatgg cctgattagt ggtttcagtg
      901 cgagtctgga ggttgatcca gaacgaaccc ggcatcgatc gggcgtcgta atgactggtg
      961 atgccatagg caaggccctg cttgtctcga atggtatcca tgagtcggga tgaaaatcct
     1021 ccggccccca acacatgatt catgacggtg acagcgtaaa aatccgggtt ggtccgactg
     1081 attccgggat ggcctatcac aatggtcgat tgggtgaggt ccttttcgat gagctgcacg
     1141 acctttttgt cgatgacagc gggcttcttg gccgttctgg gttgcaccgc ccctttcttc
     1201 caggatccga aatgggtctg gacgagcgtc gtcgcttgtt ccaccgtgac gtcaccgacg
     1261 atggtgagga tgacttgatt gggaagatac tctttggcgt agaaggtctg cacatcagcc
     1321 aaggtaatct tgcctaaggt gtcttccgtc ccgttcaccg gccaacgata gggatgaccc
     1381 tgaaagatca actgattgaa ggctttcatg gcgacatgcc caggatcatc attatcgctg
     1441 gtaatttccc caaggatctg cgatcggact cgttcgaact cttgtttggg aaaagcgggg
     1501 cgctgaagaa tatcggcgag gagcgtgaac ccgaggtcga tgtctttctt cagcgtgcgc
     1561 gccgacgcgg tcgtgaaatc ttcgtccgcc tgagctccca acgaaccacc gacaaagtcg
     1621 atctgttcgg ccagctgttt ggaggatctg gttgtggtgc cttcatcgag gagaccggcg
     1681 actaaattcg ccagaccggc cttttctgga ggatcctgcg ctgagccggc cttgatgagg
     1741 gcatggattt caacgatggg gaggaaatgt tgttgttcca acacgagaac ggtcatccca
     1801 ttcggagtgg tgaatctcgc gggtgaaatg tcagcggcat cacccggcct cgcgac
//
LOCUS       DATMTI010000234         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_51359, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000234 DATMTI010000000
VERSION     DATMTI010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51359"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..999)
                     /locus_tag="VN039_03980"
     CDS             complement(160..999)
                     /locus_tag="VN039_03980"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV45175.1"
                     /translation="MSVSEHSSGYSHALPSPDKFRYPLPVSLLDQFFVYHPEPWQDRD
                     WARVSGLPLEDVWFQASDGVRLFGWYVEAAADRPIILWCHGNAGNIINRLDNLKLLYR
                     MGLSIFLFDYRGYGKSQNIRPSEKGFYEDAYGAYDYLTRVRKIRPERIILFGRSLGAS
                     VAGELAVQRPASALMLESSFPSIEAVARLHYGGFPAHWFLKAEFRLVDRLPNLSLPKL
                     VIHGDQDDIIPIELGRQVFDAAKPPKEWYVIQGAGHNDTYAMGGGAYFRRLGEFIKKA
                     LTT"
     gene            complement(996..>2088)
                     /locus_tag="VN039_03985"
     CDS             complement(996..>2088)
                     /locus_tag="VN039_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45176.1"
                     /translation="LALLDYADPDLHLTDEFVIWTVPADTVRLIVSKPGLVSDVTYRP
                     SRSTVIKRKRESLPAAQRDLAHQITQDPGAVTSAAFIRLTLSKQAFLLDLASDYLRYR
                     IETTDSPKPEWKERNRAVLTARSQLRIPSEEFTVRPFARQPELGHKTSRASVGGGWRN
                     DDTFEEATVRAGYHDLLDPEVGYTPDAQIEMASITVRHYNRADQTRVERATLLNLLSL
                     SPIDSVFHAPSWKLNIGMNTIRHNDCRLCSNGFLNGGIGGAKEFRLLKREVLFAFAET
                     EANVSKAYHEMHRVGGGATIGMLADLTERWKVMATGTYLRFPLGDKSDDFRWYVGSRF
                     TLAQNWTVRLEYNHRDRDNDVLFSVQAFF"
BASE COUNT          486 a          582 c          555 g          465 t
ORIGIN      
        1 tggaagcagc tcgtgcggcc ggcaagccta tccccaaacc gcgttaccga cctgtcatct
       61 accaaaccgg atcgtaaggc accggtgctt cagctgtcat ttgcatgctg aacagtaaca
      121 acatgggtcc cgcattgttc agcgagaact gatctacggt catgtcgtta gcgctttctt
      181 gatgaactcc cccagccgac ggaagtaggc gccgccgccc atcgcatagg tgtcgttatg
      241 gccggcgcct tggatcacat accattcctt cggcggtttc gccgcatcga aaacttggcg
      301 ccctagctca atggggatga tgtcgtcctg atccccatga atgactagtt tcgggagtga
      361 aaggttcggt agacgatcga ccaatcgaaa ttctgcttta aggaaccaat gcgccgggaa
      421 gccaccatag tgcaatctgg ctactgcttc aatagaagga aatgacgatt caagcatgag
      481 ggctgaggca ggtcgttgca ccgcaagttc gcccgccaca gaggctccga gtgatcgccc
      541 aaagaggata attcgttcag gacggatttt tctgactcgg gtgaggtaat catacgctcc
      601 atatgcgtct tcatagaatc ctttctcact gggacggata ttctggctct tcccatatcc
      661 acgatagtcg aacaggaaaa tcgataggcc catccggtaa aggagcttga gattgtcgag
      721 acggttgatg atattgcctg cattgccatg acaccacagg atgatcgggc gatcagccgc
      781 cgcctcaaca taccaaccga acaaacggac gccatcggaa gcctgaaacc acacatcttc
      841 cagcggcaac ccgctcaccc ttgcccagtc ccggtcttgc caaggttccg gatgataaac
      901 gaagaattgg tcgagaagac tcaccggcag aggatagcga aacttatctg gggagggaag
      961 agcatggctg tagccgctcg aatgttccga tacgctcaaa aaaacgcctg tacactgaac
     1021 aagacatcat tgtcgcgatc gcgatggttg tactccagtc tgaccgtcca attttgagct
     1081 agcgtgaagc gcgacccgac gtaccaccgg aagtcgtccg acttatcccc taacggaaat
     1141 ctcaagtacg tccctgtcgc catcactttc catcgttcgg tgagatcggc caacatgccg
     1201 attgttgcac cgccgccgac tcggtgcatc tcgtgatagg ccttactgac gtttgcttca
     1261 gtttcggcga aggcgaagag gacttctcgc ttgagcaacc gaaattcctt cgcccctccg
     1321 atcccgccgt tgaggaagcc gttgctgcaa agccggcaat cgttatgccg gatggtgttc
     1381 atcccgatgt tgagcttcca ggatggagca tggaagacgg aatcgatcgg cgagagggac
     1441 agaagattca acaacgtcgc ccgttctacc ctcgtctgat ccgcccgatt gtagtgccgt
     1501 accgtgatgg aggccatttc aatttgggca tcgggcgtat agccgacttc cggatcgagc
     1561 aaatcatgat atcccgcacg aaccgtcgct tcctcaaacg tatcgtcgtt ccgccaaccg
     1621 ccccctacgc tggcccgtga cgtcttatgt cctagttccg gttgcctggc gaagggtcgc
     1681 acggtgaatt cctccgatgg aattcgaagc tgactgcggg cagtcaaaac cgccctatta
     1741 cgttccttcc attccggttt cggagaatcg gtcgtctcta tccggtagcg aagatagtct
     1801 gatgctaaat caagtagaaa tgcctgtttg gagagggtga gtcgaataaa cgccgctgag
     1861 gtcactgcgc cgggatcttg cgtgatctgg tgagccagat ctcgctgcgc tgccggcaac
     1921 gattcccgct ttcgcttgat gaccgtactt cgagacggtc ggtaggtgac atcggacacc
     1981 agacctggtt ttgagacaat caatcgcacg gtatcggccg ggacggtcca aataacgaat
     2041 tcgtcggtca agtgcaaatc ggggtcggca taatccaaga gcgcaaga
//
LOCUS       DATMTI010000235         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_51362, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000235 DATMTI010000000
VERSION     DATMTI010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51362"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VN039_03990"
     CDS             <1..532
                     /locus_tag="VN039_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HWV45177.1"
                     /translation="EAVKEILERLPPHTPPILITQHMPERFTKTWADRLNKLCRISVK
                     EAQDGDSVLPGHALVAPGNFHMTLVRSGARYTVQINQDSPVNRHRPSVDVMFSSMARY
                     AGANAIGVILTGMGNDGAKEMLAMKQAGAFTIAQDETSCVVFGMPKEAIKAGAVDKVL
                     PLNDIADAILTHVSRR"
     gene            552..884
                     /locus_tag="VN039_03995"
     CDS             552..884
                     /locus_tag="VN039_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWV45178.1"
                     /translation="MTRRVWRDATVIQIDDVFTHRNRKDFSSAVSAFREAGGKHLIVN
                     LREVTFIDSAALGLPALTSQQLAVDERRLSLVDPQGTVKQILEMANIDKMIAVFPTEE
                     AAAEARAA"
     gene            933..1283
                     /locus_tag="VN039_04000"
     CDS             933..1283
                     /locus_tag="VN039_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWV45179.1"
                     /translation="MAMHTKERPVPNGVVLEMTGDLTYANREQFKTAVETIRQKGCRH
                     LILNMAEVRFVDSSGLGLLALVSQNFKLSQGKVSMLKPQSYVREIMSLANIQKLIPVY
                     DNEQDALAGHQRAA"
     gene            1459..>2581
                     /locus_tag="VN039_04005"
     CDS             1459..>2581
                     /locus_tag="VN039_04005"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWV45180.1"
                     /translation="MPISAAVTTFRNAAEDGLRTVLVVDDEPTARMALASRLKRLGYR
                     VIEAGDGKAGLEALRRERPDLTILDWMMPEMDGPSFCEQVRQDPELLSSQILMMTSHD
                     RPEQIAEGLARGADDFLSKAASKYEITARVQAGMRTATLIRRLEDVTEQIRRKQEVLE
                     RELQSAARYVESLLPAPGTLLPGVQMVHAYRPSLGLGGDLFNVVPWSEDVMGLYLLDA
                     SGHGVSSALRSASFSTFLRGGNLLHHVGSNDPGAILTEANKQFPLTEDGNYFTIIFAR
                     LDVRARTLSYATAGHNGALLHRRSGEVRWMTKPNLPLGFDPSTTYLMEELPIGSDDRL
                     YLLSDGLYEVPASNGELWGQDRLEKTIRTFGHRPLAEVVS"
BASE COUNT          556 a          685 c          795 g          545 t
ORIGIN      
        1 ggaggctgtg aaggagatat tggaaaggct ccccccgcat accccgccca tcctgatcac
       61 gcaacatatg ccggagcggt ttacgaagac ttgggcagac cggctgaaca agctttgtcg
      121 aatctccgtc aaggaagcgc aggacggaga cagcgtcttg ccgggacatg cattggtcgc
      181 ccccggcaac tttcatatga cgttggtcag gagcggggct cgatacacgg tgcagatcaa
      241 ccaggattcc ccggtcaacc gccaccggcc ctccgtggat gtgatgtttt cgtccatggc
      301 ccgttatgcc ggcgccaacg ctatcggagt gattctcacg ggaatgggaa acgacggcgc
      361 caaagaaatg ttggcgatga agcaagccgg tgccttcacg atcgctcaag atgaaacgag
      421 ctgcgtcgtg ttcggcatgc cgaaagaagc gatcaaagcc ggggcggtgg ataaagttct
      481 gccgctgaac gatatcgccg acgccatact gacccacgtg agccgccgat gaatccggag
      541 gatttatgcc catgacccga cgggtttggc gtgacgcgac cgtgatacaa atcgatgatg
      601 tcttcacgca ccgcaaccgc aaggatttct cttccgcggt gtcggctttt agagaggcgg
      661 gagggaaaca tctcatcgtc aatctgcgcg aggtgacctt tatcgacagt gccgccttgg
      721 gcctcccggc cttgacctct caacagcttg cggttgatga gcgcaggctc agtctcgtgg
      781 atcctcaagg aaccgtgaag cagattctgg agatggccaa tatcgataag atgattgcag
      841 tgtttccgac ggaggaagcg gcggccgaag ctcgggctgc gtgaacggtg gagacgagcc
      901 gatctcgatt ggtctgaacg aaggaggtac tcatggcgat gcacacgaaa gaacgtcccg
      961 tcccgaacgg tgtggttctt gagatgacgg gagatctcac gtacgcgaat cgtgaacaat
     1021 tcaaaacggc cgtggaaacg attcgacaga agggctgtcg gcatctgatc ttgaacatgg
     1081 ccgaagtccg atttgtggac agttccgggc tcggcttgct ggcgctcgtt tctcagaact
     1141 ttaaattgag ccaaggaaaa gtgagcatgt tgaagccgca aagttacgtg cgggaaatca
     1201 tgagcctggc caatattcag aaactgatcc cggtctatga caatgagcag gatgcgttgg
     1261 ccggacacca gcgcgcagcc tagcaggatg ttgaaacagg ccgtcagcgg cgttctcgca
     1321 tctgctgttt gagtcggtca tatccctgga atcttgatcc aggatctctc gaaatagtgt
     1381 tttctcacga cctgatcgga tagaggggcg tgttcgtcga ttgaaacgct cataggtgtt
     1441 ctcatcgagt ctcatgccat gccgatttca gccgcagtga caaccttccg caacgccgca
     1501 gaagacggat tgcggactgt tttggtcgtg gacgatgagc ccaccgcacg gatggctttg
     1561 gcgtctcgac tcaaacggct gggctatcgt gtgattgaag ccggcgatgg aaaggccggg
     1621 ttggaagcgc tccgacgcga gcggccggat ctgactatct tggattggat gatgccggaa
     1681 atggacggac cgtctttctg cgagcaggtt cgtcaggacc ctgagctgct gtcgagtcaa
     1741 atcctcatga tgacgagtca tgaccggcct gaacagatcg ccgaaggcct ggccagggga
     1801 gccgatgact ttctcagcaa ggccgccagc aagtatgaga tcacggcccg cgtgcaagcc
     1861 ggcatgcgca cggccacctt gatccggagg ctcgaggatg tgacggagca aatccgaagg
     1921 aaacaggaag tcctcgaacg ggagctccag tccgccgcgc gctatgtgga gtcgttgctc
     1981 ccggcgccgg ggacgctctt gccgggcgta caaatggtcc atgcctatcg gccgtcgctc
     2041 ggtttgggcg gcgatctttt caatgtcgtg ccgtggagtg aggacgtgat gggcttgtat
     2101 ttgctggatg cgtccggcca tggggtgtca tcggcgctgc ggtctgcctc gttttcaacc
     2161 ttcctgcgcg gaggcaattt gctgcaccat gtcgggtcca acgatccggg tgcgatcttg
     2221 acggaagcga ataagcagtt tcctctgacg gaagacggga attattttac gatcatcttt
     2281 gccagactcg atgtccgagc acgtacgctg tcctatgcaa cggccggaca caatggagcg
     2341 cttcttcatc gtcggtccgg tgaggtccga tggatgacca agcctaatct gccgctgggt
     2401 tttgatccgt cgacgaccta cctcatggag gagctcccga tcggatcgga tgatcggtta
     2461 tacctgctaa gcgacggcct gtacgaagtg ccggcttcga acggcgaact ctggggacaa
     2521 gatcggctcg aaaaaacgat tcgtacgttc ggccatcgcc cgctggcgga agtggtctcc
     2581 g
//
LOCUS       DATMTI010000236         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_51521, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000236 DATMTI010000000
VERSION     DATMTI010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51521"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VN039_04010"
     CDS             complement(<1..141)
                     /locus_tag="VN039_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWV45181.1"
                     /translation="MKGVVLAGGLGTRLLPLTKVTNKHLLPVYNRPMIYYPIQTLVDA
                     GVT"
     gene            complement(258..2153)
                     /locus_tag="VN039_04015"
     CDS             complement(258..2153)
                     /locus_tag="VN039_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HWV45182.1"
                     /translation="MKQAVVKLFHSVAGRYGSTVLDHRTTLVIGVQLALILAANLTAF
                     ALRFEGDIPHEYEQIMLDHVPAVLLIFGAGLWVFGIQRGLWRYVGLHDLGKILLASLS
                     SAAVFYGVIHLIGGITQYPRSVIILTGLLNGLYLAGIRLAVRGFREWIQVVGPSARRV
                     LIVGAGHAGELLVRDMLADANYDCRPVGFVDDDPIKQRKRIHGIPVVGTIADTKKAAD
                     RLDAQEIIVAIPSSSTAVKQKILAASEGCTVPIKTLPSVKRLLGDSVLLQQVRPMNLD
                     DLLQREPIQTDRQELPPFITGKTLLVTGAGGSIGSELCRQIARHKPQALVLFERYENA
                     LHSLLLELRAAFSEVKLFPVIGDVTVLDRVSEVFHRTSPDIVFHAAAHKHVPLMELNP
                     KEAIRNNILGTRVVAEVALKTGVDRFVLISTDKAVNPSSVMGVTKRIAEHLVREFNHT
                     GLTKFTVVRFGNVLGSNGSVVPLFSEQIRKGGPVTVTHSDIKRFFMTIPEAVQLVLQA
                     SVMGRGGEVFVLDMGEQIKIADLARNMIVLAGLVPGKDVDIVFTGLRPGEKLYEELFE
                     ESEQAEPTTHPKIHRAVGTPVPVSELGEWLESLPTTLPKSDEEELLQDLKRLVPSFQP
                     DLSQSVS"
     gene            complement(2150..>2810)
                     /locus_tag="VN039_04020"
     CDS             complement(2150..>2810)
                     /locus_tag="VN039_04020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWV45183.1"
                     /translation="GTPVVSWTDEDEASIHQVFPEVVSHRRYAVLHVSPKFAYKTWSV
                     TGWVALGRWLIDQGLVVVVTGGASDQRAYSEQVVERLPDAVNLVGHLALPALGYLLSR
                     AAVYVGTDTAVSHMAAAVGAPTVVLFGPSNPVKWGPWPKDFPATAQSPWKTHGSQRLE
                     NVYLLQGEGDCVPCLAEGCDRHINSLSECLQQLPVNRVIQAVEMMLAERERRVRTSGF
                     A"
BASE COUNT          635 a          863 c          715 g          597 t
ORIGIN      
        1 cgtcacaccc gcatcgacca gcgtctgaat cggataatag atcatcggcc ggttataaac
       61 cggaagcaga tgtttgtttg tgaccttggt gagaggcaat aacctggtcc ccagcccgcc
      121 tgccagcaca acgcctttca ctgctgtacc ttcctattgc aagtggttcc ctgttgtttg
      181 catcggttgc cgttggttgg aactgtagag cttgacgata acacgaaggg gtaaaatcac
      241 cacgtgatat gcaaaaacta cgaaacgctc tgagagaggt cgggctgaaa gctgggcaca
      301 agtcgcttga gatcttgaag cagttcttct tcgtcgcttt tggggagcgt cgtcggcaat
      361 gactccaacc attcgcctaa ttcgctcacc gggacagggg tccccacagc tcgatggatc
      421 ttcggatggg tggtcggctc ggcctgctcg ctctcctcga acagctcctc gtacaatttc
      481 tctcctggcc gaagccccgt aaagacgatg tcaacgtctt ttccaggaac gaggccggcc
      541 aatacgatca tgtttctggc cagatcggcg attttgatct gttcccccat atcgaggacg
      601 aacacttctc ctccccgccc catcacgctt gcctggagaa ccaattgcac ggcctccggg
      661 atcgtcataa aaaaccgctt gatgtccgaa tgggtgacgg tcaccggacc gccttttcgg
      721 atctgctcgg aaaacagggg caccacgctc ccattgctgc ccaatacgtt gccgaaccgc
      781 acgaccgtaa atttcgtgag accggtgtgg ttgaactccc gcaccaaatg ctccgcgatt
      841 ctcttggtga cgcccatgac gctcgacgga ttgacggcct tatccgtgga gatcagcaca
      901 aaacggtcca cgcctgtttt caacgcgact tcggcgacca cgcgggtccc taggatattg
      961 ttgcggatcg cttccttcgg gttcagttcc atcagcggaa catgtttgtg cgcagcagca
     1021 tgaaatacaa tgtcagggct ggttcggtgg aatacttccg atacgcggtc gagcacggtc
     1081 acatccccga tgacagggaa cagctttact tccgaaaagg ccgctcgtaa ctccagcagg
     1141 agcgaatgaa gtgcgttctc gtatcgttcg aacaaaacta aggcttgcgg cttgtgccgg
     1201 gcgatttgcc tgcataattc cgatccgatc gaacctcccg ccccggtgac gagcagcgtc
     1261 ttcccggtga tgaaaggggg cagttcttgg cgatccgttt ggatcggctc acgctgcaac
     1321 aaatcatcca aattcatcgg cctcacttgc tggagcaaga ccgaatcgcc cagtagacgc
     1381 ttgacactcg gcaaggtctt aatcggaacc gtgcagcctt cggaggccgc gagaattttc
     1441 tgttttaccg ccgtcgaaga cgatggaatg gcgacgatga tctcttgggc atccagccta
     1501 tcggccgcct tcttggtatc ggcaatcgtc cctacaaccg gaattccatg gatccttttt
     1561 ctctgtttga tcggatcgtc atccacgaac cccaccggac ggcaatcata atttgcgtcg
     1621 gccaacatgt ccctgaccaa tagctcgccc gcatggccgg caccgacgat caagacgcgg
     1681 cgcgcgctcg ggccgacgac ctggatccat tcccggaatc cccgcacggc caaccgaatt
     1741 cccgccaaat acaatccatt caacagaccg gtcaggatga tcactgatcg aggatactgc
     1801 gtaatacccc cgataagatg gatcactcca taaaacaccg ctgcgctgct cagagaggcc
     1861 aaaaggattt tgcccagatc gtgaagaccg acatatctcc aaagccctcg ctgaatccca
     1921 aacacccata aaccggcgcc gaatatcagc aacacggcgg gcacgtgatc caacatgatc
     1981 tgctcatatt cgtgaggaat atcaccttca aagcgcaacg caaaggcggt caaattggcc
     2041 gccaggatca gagccagctg cacaccgata acaagggtcg tacgatgatc gagcaccgtg
     2101 ctcccatacc gtccagcgac ggagtggaac aatttgacga ccgcctgttt catgcgaatc
     2161 ctgatgtccg aacgcgacgc tcccgctccg ccaacatcat ctcgacggct tgaatcacac
     2221 gattcacggg caactgttgg agacattcgc ttaaactgtt gatatggcga tcgcagcctt
     2281 cggccaagca cggcacgcag tctccttccc cttgaagcag gtagacattc tccagccgtt
     2341 gcgatccatg ggttttccag gggctctgcg ctgtggcagg gaagtccttc ggccagggcc
     2401 cccacttcac cggatttgac ggcccgaaca ggacgacggt cggcgcaccg acggccgccg
     2461 ccatgtgact cacagcggta tccgttccga catatacggc tgcgcggctg agcagatacc
     2521 cgagtgccgg caatgccagg tggccgacga gattcaccgc atccggaagc ctctcgacca
     2581 cttgctcact ataagctcgt tgatccgacg cccctcccgt caccacgacc acaagtccct
     2641 gatcgattaa ccatctcccg agtgcgaccc agccggtcac cgaccaggtc ttgtaggcaa
     2701 acttcggcga gacgtgtaat acggcgtatc gtcgatgact cactacttcc ggaaacacct
     2761 gatgaatgga tgcctcatcc tcatctgtcc aactgacaac gggtgtcccc
//
LOCUS       DATMTI010000237         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_603511, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000237 DATMTI010000000
VERSION     DATMTI010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603511"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VN039_04025"
     CDS             <1..643
                     /locus_tag="VN039_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWV45184.1"
                     /translation="MADKDERTIHTVYLDAFYIDQYEITTARYAKFFQETKRSAPKYW
                     SEQVLKQHERKPVVGVDWNEAIAYCSWAGKRLPTEAEWEKAARGTDQRLYPWGNEEPG
                     RERANFDRCCDSSHYGILTDVGSFDGGKSPYGVYDMAGNVWEWVADWSDGGYDATSLE
                     RDPTGPSRGEKRVVRGGSWDSAPAYVRSSYRLRLSPTFRLDNIGFRCARDVPK"
     gene            complement(679..1011)
                     /locus_tag="VN039_04030"
     CDS             complement(679..1011)
                     /locus_tag="VN039_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HWV45185.1"
                     /translation="MDSYYHAHDLGKFSDLGKGNNALWEKFMGYYEAVFAEGALTERE
                     KALIALAVAHTVQCPYCIDAYTQASLEKGSSVEEMTEAVHVACAIRGGASLIHGVQMR
                     NVAEKLSM"
     gene            complement(1070..1351)
                     /locus_tag="VN039_04035"
     CDS             complement(1070..1351)
                     /locus_tag="VN039_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45186.1"
                     /translation="MARAFPPFEQPDKSTSNDNPADPVWDVELLRVLVSRKGTQVEAQ
                     WAIHPQLKQDLLPNEWQEVMDLMAKATDIVGDRFSKALSEADPMPPGNA"
     gene            complement(1411..>2273)
                     /locus_tag="VN039_04040"
     CDS             complement(1411..>2273)
                     /locus_tag="VN039_04040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWV45187.1"
                     /translation="KTLDFLNSTVRSCYNQTVPIPHEPISHDALLEAAVAASQEAGDL
                     LVKYAGAGFHIEYKNPINLVTDADRAAEQCVIERIKAHFPTHRFLAEERGRVEKAQSP
                     YLWIIDPLDGTTNFAHGYPTYCVSIGLEYEGRCVLGAVFDPTRNDLFTAIEHRGAQLN
                     GRPIHVSNTMGLDGSLLVTGFAYDIRETPRNNLDHFSNFALKAQGLRRTGSAALDLCY
                     VAAGRFDGFWEVRLNPWDMAAGSVIVREAGGQLTDFNGKDLSIYGQELVASNGQIHDA
                     MLAVLNQETP"
BASE COUNT          511 a          618 c          639 g          505 t
ORIGIN      
        1 aatggccgac aaagatgagc ggacgatcca tacggtgtat ctggatgcct tctacatcga
       61 ccaatatgag ataaccaccg ctcgctacgc caaattcttt caagagacga aacgatccgc
      121 gccaaagtat tggtctgaac aggtcctgaa gcagcatgaa cgcaagccgg tggtgggggt
      181 ggactggaat gaagctatcg cctactgctc ttgggcagga aaacgcttgc ccaccgaagc
      241 cgaatgggag aaagcggcgc gagggaccga ccaacgtctg tatccatggg ggaatgagga
      301 acccggtcga gagcgggcaa atttcgaccg ctgttgtgat tctagccact atgggatcct
      361 gaccgacgtc ggatcctttg atggagggaa aagtccctac ggtgtctacg atatggccgg
      421 caacgtgtgg gaatgggtgg cggattggtc cgacggaggt tatgacgcca cgagcctgga
      481 gcgcgatccg acagggcctt cgaggggtga aaaacgtgtg gttagaggtg gatcctggga
      541 ctcggctccg gcctacgttc gatcctcgta ccggctcagg ctctcgccca cgtttcggct
      601 cgacaatatt gggtttcgtt gtgcccggga cgttccaaaa taacctttac gcttgagccc
      661 ttgaacgatg ggatgcacct acatcgacag cttctcggcc acgtttctca tctgcacacc
      721 gtgaataagc gatgcaccgc cgcggatcgc acaggcgaca tggacggcct ccgtcatttc
      781 ttcgacgctc gatccctttt ccaatgatgc ctgggtgtag gcatcgatgc agtaggggca
      841 ttgcacagta tgggcaacgg ccagtgcaat gagtgccttt tcccgttcgg tcagcgcacc
      901 ctcggcaaac accgcttcat agtagcccat gaacttctcc cacagggcgt tgtttccttt
      961 cccaaggtca gaaaacttgc ctagatcatg cgcgtgataa tacgagtcca tacgaacctc
     1021 ctaggtcggt tttagcgtga aaaagaaaaa atggagcggt ggagcacaat caggcattgc
     1081 ccggaggcat ggggtcggct tcagataagg ctttggaaaa gcgatcaccg acgatgtcgg
     1141 tggccttcgc catcaggtcc atcacttctt gccattcatt ggggagcaag tcttgtttca
     1201 actgcggatg gatcgcccat tgggcctcga cctgtgttcc cttgcggctg accagcacac
     1261 gcagcagttc cacatcccag accgggtcag caggattatc gttggacgtc gacttgtccg
     1321 gttgttcaaa gggagggaag gccctcgcca taagtaaccc tcagtgtaaa tcgttcacac
     1381 ggcctactgt attctgcgtc atcctgtata tcaaggggtc tcttggttga ggactgcgag
     1441 catggcgtcg tggatctgcc cattactcgc cacgagttcc tgtccataaa ttgagaggtc
     1501 ttttccgtta aaatcggtca gctgtccgcc ggcctctcgc acaatcaccg accccgccgc
     1561 catatcccat ggattgagcc ggacttccca gaacccatcg aagcgtcctg ccgccacata
     1621 gcagaggtcc aatgccgcag atcccgttct cctgagtccc tgggccttga gggcaaaatt
     1681 ggaaaaatga tcgaggttat ttcgcggcgt ttctctgatg tcataggcga aacccgtcac
     1741 caataggctg ccgtcgaggc ccatcgtatt ggacacatgg atggggcgac cgttcaactg
     1801 cgcaccccga tgttcaatgg ccgtgaacag gtcattccgc gtgggatcaa agactgcgcc
     1861 aagcacacaa cgcccctcgt actcgaggcc gatggagacg cagtaggttg gatagccatg
     1921 cgcaaaatta gttgtcccat ccagcggatc gatgatccac agataaggcg actgcgcctt
     1981 ttcgacgcga cctcgttctt cggccaagaa ccggtgggtt gggaaatgag ccttaatacg
     2041 ttcgatgaca cactgctcgg cagcacgatc tgcgtcagtg actagattga tcgggttctt
     2101 atactcgatg tggaaaccgg ctccggcata tttaaccagc aggtcgccgg cttcttggct
     2161 ggcggcaacc gctgcttcga ggagcgcgtc atgcgagatt ggttcatgag gaatcggcac
     2221 cgtctgattg tagcatgacc gaaccgtaga attgagaaaa tcaagagtct tgt
//
LOCUS       DATMTI010000238         4068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_9611, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000238 DATMTI010000000
VERSION     DATMTI010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9611"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /gene="gst"
                     /locus_tag="VN039_04045"
     CDS             complement(<1..394)
                     /gene="gst"
                     /locus_tag="VN039_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWV45188.1"
                     /translation="MKNFALYYWPVPFRGQFIRGILAHCGYSWDEYDVDAIEGIMDCG
                     VEKQPVAFMGPPVLIDRERNFAISQMPAIAIYLGERLDMLPTTVEGRALSAKIVNDAN
                     DVLDELTLDGGREMWTPEKWEEFIPRFQK"
     gene            complement(642..2399)
                     /gene="ggt"
                     /locus_tag="VN039_04050"
     CDS             complement(642..2399)
                     /gene="ggt"
                     /locus_tag="VN039_04050"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWV45189.1"
                     /translation="MKMRFLSEAVLALMLSTAIASAQQASDTVAPEKATDVATAKRIE
                     SKSFMVAAANPLAAEAGRDVIATGGNAIDAMVAVQAMLGLVEPQSSGLGGGAFLVYYD
                     AKSGKLTTFDGRETAPMEATPKLFLDDNGQPLKFMDAVVGGRSVGTPGTVRLLEEAHK
                     RYGKAEWASLLKPAETLATEGFKVSPRLASLIASEGDRLKKYKEARSYFFDTSGAALQ
                     TGALLKNPAYAETLAVLAKGGADAFYRGRIAEAVVDTVRGAPDNPGVLSLTDLSNYRV
                     IEREPVCLNYRALDVCGMGPPSSGAIAVGQILGLVENFDLRSLGPKNIESWRIIGDAQ
                     RLAFADRERYVADTHFVPLPIKGLLDKSYLGERAKLLDGDKALANDAVKAGEPQWDHA
                     LVFGRDTALELPSTSHFVIVDKDGNVVSMTTTIENGFGSRLMTNGFLLNNELTDFSFK
                     THDGGLPIANRVEPGKRPRSSMAPTIVMKDGRPLLAIGSPGGSQIIGYVTQALIAYID
                     WGMPVEAIVAQPHLINRFGTYDIEAGTSAEELIGPLKALGYEVKPGEMNSGLHAIEIT
                     AQGLAGSADPRREGMVVGE"
     gene            3039..>4068
                     /locus_tag="VN039_04055"
     CDS             3039..>4068
                     /locus_tag="VN039_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45190.1"
                     /translation="MENPIALNSLSENTVFRKGDVFVLFGELFGRGYATGLLDEARRA
                     GMEIVGITVGRRDENNALRPLDAEELSSAEAQLGGRIINIPLMAGFDLDAPADGPTPT
                     DLLAKMTLESWEHDKLDWDYVGQCRDIATARFTESLSRVMTVLDGMIGAGRNVFFAHT
                     MAGGIPKAKVFLVVANRIYKGTGARHMSSQTLLDSDMGKLILQNFDDVSANTFRHLID
                     FSRAIRERVEASGGQVRYTAYGYHGSAVLIDGSYRWQTYTNYTQGYAKMRLEGIAEEA
                     WATGIKATVYNCPEIRTNSSDVFTGIELPLIPLLLALKKENGGQWADEQWQACQQLLA
                     DGLTMKDVF"
BASE COUNT          852 a         1198 c         1136 g          882 t
ORIGIN      
        1 atttttggaa acgagggatg aattcctccc acttttcagg tgtccacatt tctcgcccgc
       61 cgtcgagagt caactcatca aggacatcat tggcatcgtt aacgatcttg gcgctgagtg
      121 cgcggccctc aacagttgtc ggcagcatat ccagccgctc accgaggtat atggcgatcg
      181 ccggcatctg ggagatcgca aaattgcgct cacggtcaat cagaaccggt ggccccatga
      241 aggcaaccgg ctgcttttcc accccacaat ccataattcc ttcaatcgca tcgacatcgt
      301 attcatccca ggagtaccca caatgggcca gaattccgcg gatgaactga cctcgaaaag
      361 gaactggcca gtaataaagg gcgaagttct tcatgccgtc ctccattggt catgcagaca
      421 actaacggag tgggttacaa ccagttcccc gacgtgcact caggaattct ctgacgcttt
      481 gacggcttca atggcgcagg aagagttgtt tttggacgta atgtcctgtt ccctacgatt
      541 tcctcgctaa aaacaggaca cccacgcggg gtagtcacct agcctccaca cgacgctatg
      601 ctctgtctct tgaggccgcc gtcttttcgc aggccgggcc ttcactcccc tacaaccatt
      661 ccctcgcgtc gtggatcggc gctgccggcc aagccctggg cggttatctc gatagcatgt
      721 aaaccggaat tcatctcgcc cggtttcacc tcgtaaccca gcgccttcag cggcccgatc
      781 agttcttccg cgctggtgcc agcctcgatg tcgtaggtcc cgaaccggtt tatgagatgc
      841 ggctgagcga cgatcgcctc aaccggcatg ccccagtcaa tgtaggcgat cagcgcctgt
      901 gtcacatagc cgatgatctg gctgccgccg ggggaaccga tggcgagcag gggtcttcca
      961 tccttcatca cgatagtcgg ggccatggag gagcgcggcc gtttgccggg ctcaacgcgg
     1021 ttggcgatcg gcaggccgcc gtcatgggtc ttgaaggaga agtccgtcaa ctcgttattg
     1081 agtaggaagc cgttcgtcat cagacgcgag ccgaagccat tttcgatcgt cgtcgtcatc
     1141 gagaccacgt tgccatcctt gtcgacgatg acaaaatggc ttgtggaagg caactcgagc
     1201 gcggtatcac ggccgaaaac tagcgcgtgg tcccattgcg gctcgccagc cttgacggca
     1261 tcgttggcca gcgccttgtc gccgtccagc agctttgccc tctcgcccag ataggacttg
     1321 tccaacagac ccttgatcgg cagtggcacg aaatgggtat cagcgacgta acgctcgcgg
     1381 tcggcgaagg cgagacgctg cgcatcgccg ataatgcgcc agctttcaat gtttttcggc
     1441 cccaggctcc ttaaatcgaa attctccacc aggccgagta tctggccgac ggcgatggcg
     1501 cccgaagagg gcggtcccat gccgcacaca tcaagagcac gataattgag acagacaggt
     1561 tcgcgctcga tcacgcgata gttggacaga tcggtcagcg acagcacgcc gggattgtcg
     1621 ggtgccccac gcacagtgtc gacgaccgcc tcggcaattc tccctctata aaaagcatcg
     1681 gcaccccctt tggcgagcac cgcaagcgtt tcggcatagg ccgggttctt caacagggca
     1741 ccggtctgca gtgcggcccc ggaggtgtcg aagaaatatg acctcgcctc tttatatttc
     1801 ttcaaccggt cgccctcgga cgcgatcagg gaggcaaggc gcggtgacac cttaaagccc
     1861 tcggtagcca gcgtttccgc cggcttcagg aggcttgccc actccgcctt tccatagcgt
     1921 ttgtgggctt cctccagaag ccgcactgtt cccggtgtgc caacggaacg gccgccgacc
     1981 actgcgtcca tgaatttaag cggttggccg ttgtcatcca ggaagagctt tggcgtggct
     2041 tccatcggcg ccgtctcgcg gccgtcaaac gttgtcagct tgccgctttt tgcgtcgtag
     2101 tagaccagga aggccccgcc gccgagaccg gaactctgcg gttcaacgag gccgagcatg
     2161 gcctgcaccg ccaccattgc atcgatggcg ttaccgcccg ttgcaatcac gtcgcgcccc
     2221 gcctcggccg ccagcggatt ggccgccgcc accatgaagc tctttgactc gatacgtttg
     2281 gcagtcgcaa catccgttgc tttctcgggc gccaccgtgt cggacgcctg ctgcgcggag
     2341 gcgattgcgg tggaaagcat caaggcgaga acagcctcac tgagaaatcg cattttcatt
     2401 agccttatcc tccagagagg gacaaccagt tcatgcaagt agtgtatcaa accgagccag
     2461 cacgcaggcc gaacctgtat cgaccaaggc gcggaccgtt gtccggactg ttccttcgtt
     2521 cccgcaaagg ttcggccttc accggtattt cggccccgtc cgttggttcg ccccgctatt
     2581 gagcctcggt ctggtacgcc gctttttcga cactatggtg gctgtttcgc cgcgtgtcca
     2641 tcatgtcgaa gggtcgacat atcgttggaa acggagcctc cgttaacagc agtgtcgcca
     2701 gttcgatttg taccaagaac tttgtaaatt gcatgagtct cgatgcaagc cgcctcccgg
     2761 cacaaaaaaa tagggttgct ttgcgcctca ggaaacgccg atagcctctc aagcatccgt
     2821 agacgagcgt ttcgaagtaa ttcgttttgg tatcggtgcg cctatctcca acggctttgg
     2881 catttcgctg cagtgtaaga acaacagggg attgatttca cgttttgaaa tgaacggtca
     2941 ccgaaaatga gttcccatcc ggcgactatg cggtcatgtc tttatcacag gtcgtgtgag
     3001 ccattgctct acgatacgcg aggcaaagag ttgacagtat ggaaaatccg attgcactga
     3061 atagtctttc cgaaaacacg gtcttccgca agggcgatgt tttcgtattg ttcggcgaac
     3121 tattcggccg cggatacgcc accggcttgc ttgacgaagc caggcgggct ggaatggaga
     3181 ttgtggggat caccgtcggg cggcgcgacg agaacaacgc gctccggccg cttgacgccg
     3241 aggaactgtc ttcggcggag gcccagctgg gcggcaggat tatcaacata cctcttatgg
     3301 cgggtttcga tctcgatgcg cccgcagacg gtccaactcc caccgatctc ctggcaaaga
     3361 tgacactgga gagctgggaa cacgacaagc tcgactggga ctatgtcggg caatgccgcg
     3421 acatcgctac tgcgcgtttt acggagtcgc tttcaagggt catgaccgtt ctagacggaa
     3481 tgattggcgc tggccgcaat gttttcttcg cccacacaat ggctgggggc attccgaagg
     3541 cgaaggtatt cctggtcgtc gccaatcgaa tctacaaggg aaccggcgcc cgccacatgt
     3601 cttcgcagac cctgctcgac agtgacatgg gaaagctcat tctacagaat tttgacgatg
     3661 tctccgcaaa cacctttcgc catctcattg atttcagcag ggcgatccgc gagcgcgtcg
     3721 aagcttcggg cggacaagtg cgatatacgg cctatggtta tcatggatcg gcggtcctga
     3781 tcgatgggag ctatcgttgg cagacctata ccaactacac ccagggatat gcgaagatgc
     3841 ggctcgaagg cattgcagag gaagcctggg cgacagggat caaggcaact gtctataact
     3901 gtcccgaaat ccggaccaac tcgtccgatg tgttcacggg tatcgagctg cccctcatac
     3961 cgctgctgct tgcgttgaag aaagaaaacg gtggccaatg ggcagacgaa cagtggcagg
     4021 cctgccagca gcttctggcc gatggcttaa ccatgaagga tgttttcc
//
LOCUS       DATMTI010000239         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_603522, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000239 DATMTI010000000
VERSION     DATMTI010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603522"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VN039_04060"
     CDS             complement(<1..710)
                     /locus_tag="VN039_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWV45191.1"
                     /translation="MDVAVTAKEIKRDDLLQMYHYLRLTRALEDRITALYRQGRIVGG
                     VYTSHGMEAIAVGYASALERDDIIAPFHRDMGAFLIRGFTTGEILAQYLGKRTGPSKG
                     KDGNVHMGDIKRGVFGFVSHLADNLPVAAGAALAFKIRGESRVVFTGTGDGGTSRGDF
                     HEAMNLAAVRRLPVVFFCNNNQYAYSTPLRLQMATPNVVDRAKAYGMPGEIVDGNDVA
                     AVYLAAQHAIARARAGEGP"
     gene            complement(732..1484)
                     /locus_tag="VN039_04065"
     CDS             complement(732..1484)
                     /locus_tag="VN039_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45192.1"
                     /translation="MPVSTIEQALAEVVDRHRVLYEDRTIDARLRAIIRAGGHTNVTE
                     DDVRHILRRSRSVFFDTHVEVVSGHHTATYLRFESIARCPELIRQLTDDIADWIRKTF
                     QHHPITGLVATSSAAEMLAQGIADVLHDTMGLRVALTPYSCETGRIGTDINVGAINAG
                     ERFLALNDVTTRGNCVSKLGKVITDHGGLLAGMMVFARRDSGQFPLMNELIARHPFYY
                     TTDLDMPQWEPEHCPLCRGKIPLLSWKDMPAL"
     gene            complement(1496..2479)
                     /locus_tag="VN039_04070"
     CDS             complement(1496..2479)
                     /locus_tag="VN039_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWV45193.1"
                     /translation="MKLVSFRIKTPIGTFTRVGATHGQHIVDLNMAYVRWLADQQEAE
                     PRRLADAQVPPTMLEFLEGGNSTLAAARRAKDYVTTLSPSAKGPSGETIVYAASDVQL
                     AAPLPDPPSLRDFIAFEDHIAATSKKRGQPIPPEWYKAPVYYKGNHRTIIGPDEALSW
                     PLETTKLDYELELACVIGRKGRDISERQAEDYIAGYTIMNDFSARDIQFQEMACRLGP
                     AKGKDFATALGPCLVTPDEIADLGALTMIARVNGEEWSRGRFGTIHWSFAQMIAHVSR
                     GETIYPGDVFGSGTVGGGCGLELDRYLKPDDVVELDIQPIGVLKTRIVSAM"
     gene            complement(2476..3105)
                     /locus_tag="VN039_04075"
     CDS             complement(2476..3105)
                     /locus_tag="VN039_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45194.1"
                     /translation="MTMLDRGLLGQHALFDKGVLLAPELETVEPAETGFQTWEVRIKR
                     QGEWTGVTYPFYPMDVVGWKGDLWVAKLNVRDFRPVVSPRYHLPPSVHQTFQAGGCLI
                     STFAPRPLESDPEALRVPFYHRNTDYDEVLFYHDGEFFSRAEIQAGMLTLHPQGIHHG
                     PQPLAVQAGKTKTHTSEVAVMIESKSPFTVQPEMESVEIKDYALSWSRR"
     gene            complement(3130..>3313)
                     /locus_tag="VN039_04080"
     CDS             complement(3130..>3313)
                     /locus_tag="VN039_04080"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HWV45195.1"
                     /translation="RRETTPYFVRNADGDEVHFVHRGRGRFETDYGVLSYEAGDYVLI
                     PKGTTYRVHVDTEPSF"
BASE COUNT          697 a         1001 c          872 g          743 t
ORIGIN      
        1 ggcccttctc ctgctcgggc tcttgcaatc gcatgttgtg ccgccagata aactgcggcc
       61 acatcattgc cgtccacaat ttcaccaggc atgccatacg ctttcgctcg atccacgacg
      121 ttcggagtcg ccatctgtag acgaagcggc gtcgagtagg cgtattgatt gttgttacag
      181 aaaaagacga cgggaagcct ccgcaccgcc gccaagttca tcgcttcgtg aaaatcgcct
      241 cggctcgttc ctccgtcacc cgtacccgtg aacacgacgc gggattcccc tctgatcttg
      301 aacgccaacg ccgcgccggc cgccaccggc agattgtccg ccagatgact gacaaatccg
      361 aagactcctc gcttgatatc ccccatgtga acattgccgt ccttcccttt gctcggcccc
      421 gtccttttac cgagatactg ggcgaggatt tcgccggtcg tgaaacctcg aatgaggaag
      481 gcacccatgt ctcggtgaaa gggagcgatg atatcgtcgc gctcaagcgc ggaggcataa
      541 ccgacggcga tcgcctccat tccgtgactg gtatagactc cgccgacaat acgtccctgt
      601 cggtagaggg ccgtaatgcg gtcctccagc gctctggtga gtcgaaggta atggtacatc
      661 tggagcaggt cgtctcgctt aatctccttg gcggtgactg caacgtccat aacagcctcc
      721 acgtcgctgc tttacaaggc gggcatatcc ttccatgaca acaatggaat tttccccctg
      781 cacagtggac agtgctccgg ctcccattgc ggcatatcga gatccgtcgt gtagtagaag
      841 ggatgtcgtg cgattaattc gttcatcaac gggaactgac cgctgtcacg ccgggcaaag
      901 accatcatcc ctgccagcaa acctccatgg tcggtaatga ccttgccgag tttgctgacg
      961 caattgcccc gcgtcgtgac atcgttgagc gctaaaaagc gctcgccggc gttgatcgcg
     1021 ccgacattga tgtccgtccc gatccgtcct gtctcacaac tgtatggcgt cagtgccaca
     1081 cgcaacccca tcgtatcgtg aagaacatcc gcaattcctt gtgcgagcat ctccgctgcg
     1141 gaactggtcg ctacgagacc cgtgattgga tggtgttgaa acgtcttcct gatccaatcc
     1201 gctatatcgt cggtcaactg acggatcaat tcaggacaac gagcgatgga ctcgaaacgc
     1261 agatatgtcg ccgtgtggtg acctgacaca acctccacgt gagtatcgaa aaagacggat
     1321 cgggacctac gcagaatatg acgcacatca tcctcggtga cattggtatg gccaccagcg
     1381 cggatgatgg ctctcagtct tgcatcgatc gtccggtctt cataaagcac gcgatgccga
     1441 tccaccacct ctgcgagggc ctgctcaatc gtgctgaccg gcatctttcc gctccttaca
     1501 ttgcactcac aattcgagtc ttaagaaccc cgatcggctg aatgtccaac tccaccacgt
     1561 cgtccggctt caagtaacgg tccagttcca gtccacagcc ccctcccact gttcccgaac
     1621 cgaagacatc acccggatag atcgtttcgc ctcgcgacac gtgcgcgatc atctgagcaa
     1681 aagaccagtg gatcgtcccg aatcgtcctc gcgaccattc ttctccgttg acccttgcga
     1741 tcatcgtcaa ggcaccgagg tcggcaatct catcaggagt tacgagacag gggcccaacg
     1801 ccgtcgcgaa gtccttgccc ttcgccggac ccaatcgaca ggccatttct tggaattgaa
     1861 tatcgcgcgc gctgaagtcg ttcatgatcg tgtaaccggc aatgtagtct tccgcctgcc
     1921 gttcagagat gtctctgccc ttccgcccga tgacacaggc cagttccaac tcgtagtcca
     1981 acttcgtcgt ctccagcggc caggacagcg cctcgtccgg cccgatgatc gtgcgatgat
     2041 tccctttgta gtagaccgga gccttgtacc actccggcgg gatcggctgt ccccgcttct
     2101 tggaagtcgc cgcaatgtgg tcttcaaacg cgatgaaatc acgaagcgaa ggtggatcgg
     2161 gaagaggggc agcgagttga acatcagagg ctgcatacac gatcgtctca ccggatggac
     2221 ctttggctga tggagagagt gtggtcacat aatctttcgc tcggcgggcc gctgccagcg
     2281 tggaattccc cccttcgagg aactccaaca tggtcggcgg aacctgagcg tcggctagtc
     2341 ggcgtggctc tgcttcctgt tggtctgcca accatcggac gtaggccatg ttcaaatcca
     2401 cgatgtgttg cccgtgcgtc gctcccactc tggtgaatgt accgatagga gttttgattc
     2461 gaaaactgac gagcttcatc gacggctcca actcaatgcg tagtccttga tctccaccga
     2521 ctccatttcc ggctggaccg tgaagggaga tttggattcg atcatcaccg ccacttcgct
     2581 ggtgtgcgtc ttcgttttgc cggcttggac cgccagtggt tgtggcccgt ggtggatacc
     2641 ctgcggatgt aacgtgagca tgcccgcctg aatctcggca cggctgaaga attctccgtc
     2701 atgatagaac agcacttcat cgtagtcggt atttcgatgg taaaacggga cgcgcaaggc
     2761 ctcggggtcg ctttccagcg gcctcggagc aaaagtggaa atgaggcatc ctccggcttg
     2821 gaacgtctga tggacgctcg gcggcaggtg gtagcgggga ctgacgaccg gccggaagtc
     2881 tcgcacgttc agcttcgcaa cccagagatc tcctttccac ccgactacat ccatgggata
     2941 gaagggatag gtcacccctg tccactcacc ttgacgtttg atgcggactt cccatgtttg
     3001 gaatccggtt tccgccggct ctactgtctc cagctcggga gcaagcaaca ctcctttgtc
     3061 gaatagcgca tgttgtccga ggaggccgcg atccagcatc gtcataggaa tgggagtctc
     3121 cacaatcagc taaaaagaag gttccgtatc gacgtgaact cgataagtcg tgcctttggg
     3181 gatcaacaca taatcacctg cctcgtagga aagaacgcca taatcggttt caaatctacc
     3241 tctgccccga tgcacgaaat ggacttcatc tccatccgca ttgcgcacga aatagggcgt
     3301 cgtctctcgt cgg
//
LOCUS       DATMTI010000240         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_136843, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000240 DATMTI010000000
VERSION     DATMTI010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136843"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VN039_04085"
     CDS             complement(<1..154)
                     /locus_tag="VN039_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWV45196.1"
                     /translation="MDWNRVEGNWKQVKGKVKEQWGKLTDDDLDQIAGKREQLEGKLQ
                     QRYGYQK"
     gene            261..>2116
                     /locus_tag="VN039_04090"
     CDS             261..>2116
                     /locus_tag="VN039_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV45197.1"
                     /translation="MSERSAEQELKERERELEEVQRIAKVGGVVVDLLHGFRNQRSPE
                     YLAIHGLPPEAKNESHSDWVNRIHPEDRERAEQQFLDALKGTSERYSSEYRIIRPSDG
                     ETRWIAAEGRIERDASRRAIRLVGAHIDITERSLAREMLRESEERFRLIADSAPVPIW
                     VTQMDRTRSFANRAYVEFIGLPYEEALAFDWRTILHPDDMARIVQESIAGEASLKPFV
                     LEARYRRADGMTRWIRSESQPRWDPAGRHIGFIGVAHDITVAKQAEIDLRLVNDRLER
                     LVTDKTEQLRFREAQLRAILETTNQHLVLLDLEGRLVYANRTASTAAGSNFNDVIGRL
                     YWQTSWFEATSGSAKVVERMFREASSGNAARAELELQLAEGSRYIDIALRPVSDEAGN
                     ITAVLAEGVDTTERRLNEEALRQAQKMEAVGQLTGGVAHDFNNLLTIIRSATDFLRRR
                     GLPEDRRRRYVDAISDTTDRASRLTAQLLAFARRQPLAPVTFDVVAQIHSVAALIRPL
                     VGGRITIEIEAGLEGLFALADIGQFETALVNLAVNARDAMGGEGRIVISSRPADTIPP
                     VRSHQARPGDFVAISIADTGVGIPVEHLQVIFEPFFTTKDVGKGTGLGLSQV"
BASE COUNT          455 a          585 c          607 g          469 t
ORIGIN      
        1 ctttctgata cccgtatcgc tgctggagct ttccttcgag ttgctcccgt tttccagcga
       61 tctgatccag gtcatcgtcg gtgagtttgc cccactgttc tttcaccttg cctttgactt
      121 gcttccagtt tccttcgacc cgattccaat ccatagtaac tctccttttt tgtgtgattt
      181 ttaacggcca actagctcga agcgttcctg tgttacgttt tgaacttaag catggcaaat
      241 tgattggcgg cgagggagca gtgtccgaac gatcggccga gcaggaactt aaagagcgcg
      301 aacgcgagct tgaggaagtg cagcggatcg ccaaggtggg cggcgtagtt gtcgacctgc
      361 tacatggttt tcggaaccaa cgatcccctg agtatctggc tatccacggc ttgcctccag
      421 aggccaagaa cgaaagtcat tcggactggg tgaatcggat tcatccggag gatagagagc
      481 gcgccgagca gcaatttctc gatgcgttga aggggacatc cgagcgatat tcctctgaat
      541 atcgcatcat tcggcccagc gacggggaaa cgcggtggat cgccgctgaa ggccgaatcg
      601 agcgtgacgc tagtagacgc gcgatccgtc tagtcggcgc gcatatagac atcacagaac
      661 gttcgttagc gcgcgagatg cttcgggaaa gtgaggagcg tttccgcctg atcgcagaca
      721 gcgcgccggt gcccatttgg gttacgcaga tggaccgaac tcgcagcttc gcaaaccgtg
      781 cctatgtgga attcataggc cttccgtacg aagaagctct ggcctttgac tggcgaacga
      841 tcttgcatcc tgatgatatg gcgcgaattg ttcaggagag catcgcaggt gaagcttccc
      901 taaagccctt tgtcctcgaa gcgcgttatc gccgcgctga cggcatgacg aggtggatcc
      961 gatcggaatc gcagccgcga tgggatcctg caggcaggca tatcggtttc atcggcgttg
     1021 cccatgacat tacggtagcc aagcaggccg aaatcgacct tcgattggtg aacgatcgtc
     1081 tcgaaagact ggtgacggat aaaactgaac agcttcgctt cagagaagcg cagctccgag
     1141 caattctgga gacaacgaat cagcatctcg tgctgcttga tctcgagggt cggttagtct
     1201 acgcaaaccg taccgcttca accgccgcag gatcgaactt caacgacgtt atcgggcgac
     1261 tctactggca aacgagctgg tttgaagcga catctggatc tgccaaagtt gtcgaacgta
     1321 tgtttcgcga ggcctcctca ggtaacgccg cgcgagccga actcgaattg cagctagcgg
     1381 aggggagccg ctatatcgat atcgcgctgc gcccggttag cgatgaagca ggcaacatca
     1441 cggcagttct cgcagaaggt gtcgacacaa ccgaacgacg tctcaacgag gaggcattgc
     1501 ggcaagcgca gaagatggaa gccgtgggac aactcaccgg cggggtcgcc catgacttca
     1561 acaatctcct gaccattatc cgatctgcta cggattttct tcgtcgccgc ggccttcctg
     1621 aagaccgtcg tcgccgttat gtggatgcca tctcagatac cacggaccgc gcatcacgtc
     1681 ttacagccca attgctagcc ttcgctagac gccaaccgct tgcgcctgtg acgttcgacg
     1741 tcgtggcaca gatccatagc gtcgccgcgc tgatccgccc gctcgttggg gggcgcatta
     1801 cgatcgagat cgaggctgga cttgagggtc tattcgcgct ggcggacatc ggtcaattcg
     1861 aaacggcgct tgtaaacttg gcggtgaatg cacgggatgc aatgggcggc gaagggcgaa
     1921 ttgtcatctc gtctcgtccg gccgacacta tcccacctgt ccgatcgcac caggcgcggc
     1981 ccggagattt cgtggcgatc tcaattgccg acaccggagt cggtattcca gtcgaacacc
     2041 tgcaggtgat attcgagccg ttcttcacga cgaaggatgt cggcaagggt actggactcg
     2101 gccttagcca agtctt
//
LOCUS       DATMTI010000241         5961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_773294, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000241 DATMTI010000000
VERSION     DATMTI010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773294"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..>5961
                     /locus_tag="VN039_04095"
     CDS             49..>5961
                     /locus_tag="VN039_04095"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HWV45198.1"
                     /translation="MPLSSSQQRLWFLEQVHPGSAINHISMDVRLRGSVNPKVLERSV
                     REIVRRHEILRTRFGSERGEGFAEVSSEAIVTIGRQDFQGLDPTEQEMQVRQFLRAER
                     SRPFDLRQGPLFRVTLLTLEPAVHVLALTFHRLVADGWSFRIFWKELALLWEAGGDVH
                     EARLPALTVQYADYADWQRIRLDQGLREVHRAYWIRQLSGVHPPAELPVDRQRPRVRT
                     FEAGLRSRSLSPKLSADLDLFCQHQNATTFMVLYAVFATWLHRYTQESDVVIGSIVAG
                     RHRRELEDVMGYCVNTVALRSELSDGMTGRELLKQVRRVVVDAYDHQELPFEEVIEAL
                     SLQRARQLSPLFNVMMVCEEDPLSSFTVKNLEIAHLPWEPTAAEFDLVLMVVNKVDGL
                     DLALLHNSTIFDDSTIDRMLGQLEILLEEFPKKSDARLDQLSLLTADEKRHIQLTWNK
                     PPHSVSGIHTLFEAQVERTPHAPAVTCGDQSISYQELNRRANRAARALRKFGVCADLP
                     VGLCVERSVDALVGLLGILKAGGGYVPLDPSFPGHRLQLMLEDAHVSVVVTQAHLRNH
                     LQGYGGQICDVETLSTSTVGAAEEENLALSISPDQLAYIIYTSGSTGRPKGVAVTHHG
                     LVTSLHARLQYYPDPASRFLLTFSLAFDGSVTGIFWTLLQGGELVIPSETAHRDPTEL
                     AALIERHRISHVVWVPSLYHAVLGEVLSTQLESLRIIVTAGESLPLELVRRHYQLLPH
                     AVLYNEYGPTEATVWCSVYQTTREEAGARVPIGKPIDHMQLYVLDASLQPMPIGIPGE
                     LYIAGDCLARGYVNHPQLTQERFIANPYVPGTRLYRTGDLARYRADGNIEFLGRTDQQ
                     VKLRGYRIELGEIEYVLSNFPGVHQAAVLLRQDKPDQHRLVGYIAGEPSLRERLDQVR
                     SHLAARLPHYMVPSAILWLETMPLSATGKVNRHALPAPEDTAGRATARIAPRNQVEES
                     LAALWKSVLQVPEAGIHDHFFEHGGHSLVATQLVSRLREIFEVDVSLQALFERPTIAA
                     LAEEVIRLRQRETASLMPPIIPVPRDQPLPLSYSQQRMWLMHRLAPQSTAYNMPFASR
                     QMGRLNKTALRSTIDAICSRHEAFRTTFMMRGEGPVQMVHPFRSPHWVEVNLRELPLE
                     QRRLQAARLVEQESNQPFDLEKGPLARFFLIEIEPEDHVLVLTMHHIIGDQWSFGVIG
                     HEFAFFYNAFCRGDVPSPKPIRLQYADYAVWQRRCLTDDRLSTQSDYWQKKLAGLSKL
                     SLPTDYPRPVVQTFNGSHCMLELPTSLIERLKQFSAEHNATVFMTLLACFQILLSRYS
                     GQTDVAVGSPIANRTQSAVESIIGSFVNTLVLRTDLSGDPTFIGLMVRVRETALEAYA
                     NQDFPFDKLVETMHSARDHSSAPLVQVLFNVPNAPIGEINVQGLSWVPFEVETQAAQF
                     DLSLTIETEFSRRAYLTFNTDLFEPQTAERMLGQYKILLMTALANPQSKLSELPTLTA
                     PERQQMLLDWNRTQRDYPRSECFPQLFEAQVERTPDAVALSMGRETLRYGELNARANK
                     LARYLQMLGARPGVAVGIGLERSLEMVTALLAVLKTGAAYVPLDPDYPRDRLRYMSEN
                     ASLIIVLTSESLAERFDVHVCRRLCLDREEKSIAQEADHDLGPIATPQDLAYILYTSG
                     STGQPKGVEIPHQALVNFLCSIRQEPGCSAQDVMLSVTTLSFDIAGLELYVPLLAGGR
                     VEIAGRAVAMDGRQLLTLCEAVQPTIMQATPATWRMLVEAGWQGSTRLTVLCGGEPLP
                     PDLAASLLDRSVALWNMYGPTETTIWSTIERIERTAQEITIGRPIANTEVYILDQFLQ
                     PVPVGVSGELYIGGHGLARGYRGRPDLTQERFIPHPFSQEPLARLYRTGDLARYRPDG
                     RIVHLGRLDHQVKIRGFRIELGEIEAALSRHQAVRQAVVLARDDQQGVKQLVAYVVCQ
                     DSPVPNQQELRSF"
BASE COUNT         1212 a         1681 c         1726 g         1342 t
ORIGIN      
        1 cggattccac ctgttccgct tctctcggca tctgcgaggg aaggcaagat tcctctcagc
       61 tcctcgcagc agcgactgtg gttcttggag caggttcatc ctggaagcgc gatcaaccat
      121 atttccatgg atgtgcgtct acgtgggtcg gtcaacccca aggtgttgga gcggtcggtt
      181 cgggaaatcg tccgtcgtca cgagattctt cgaactcgtt tcggatcgga acgaggcgaa
      241 gggttcgcgg aagtttcctc tgaggcgatc gtcacgatag gacggcaaga ttttcaaggg
      301 ttggacccca cagagcaaga aatgcaggta cggcaattcc tacgagcgga gaggagccga
      361 ccgttcgact tacgacaggg gccgctattt cgagtgaccc ttctgacgct ggaaccggct
      421 gtgcatgtcc tggccctgac attccaccgt ctcgtcgccg atggatggtc tttccgtatc
      481 ttctggaagg aattggctct tctttgggaa gccggtggtg atgttcacga agctcgactt
      541 cccgcgttga ccgttcagta tgcggactat gccgattggc agagaatcag gctggatcaa
      601 ggccttcgtg aggtccatcg agcctactgg atcaggcagt tgagcggcgt ccacccccct
      661 gctgagctac cggtcgatcg tcagcggccg cgggtgcgca cattcgaggc aggcctgcgg
      721 tcgcgatcgc tttctccaaa actttcagcc gacctcgatc tcttctgtca gcatcagaac
      781 gccacgacgt tcatggtgct gtacgccgtc tttgcgactt ggctgcatcg ctacacgcaa
      841 gagtcggatg tcgtcattgg atcgatcgtg gccggcaggc atcggagaga gctcgaagac
      901 gtgatgggat attgcgtcaa tacggtggcg ttgcggtccg agctttcgga tgggatgaca
      961 ggacgggagt tgttgaagca ggtacgccgg gtggtggtgg acgcctatga ccatcaggaa
     1021 ttgccgtttg aagaggtcat cgaggcgctg tcgttgcagc gagcgcgtca gttgtccccg
     1081 ctgtttaacg tgatgatggt gtgtgaagag gaccctctgt cctcgttcac cgtcaagaat
     1141 cttgagatcg cccatctccc ttgggagccg actgcggcgg agttcgacct cgtgctgatg
     1201 gtagtcaaca aggtggatgg actggacttg gcgttgctcc ataattctac gattttcgac
     1261 gactcgacga tcgaccgcat gttaggacag ctggagatcc ttctcgagga gtttcccaag
     1321 aaatccgatg cccgccttga ccaactttca ttgctgaccg ccgatgagaa acggcacatt
     1381 caactgacgt ggaacaagcc gcctcattcg gtctccggta tccatacgct ctttgaggcg
     1441 caggttgagc ggactcccca tgcacccgct gtcacctgtg gcgaccagtc catcagctac
     1501 caggaactga accgacgagc gaatcgcgcc gcccgcgcgc tccggaaatt tggggtctgc
     1561 gcagatctcc cggtgggtct ctgtgtcgag cgctcggtgg atgccctcgt aggcctcttg
     1621 ggtattctca aagcaggggg cggttatgtt cccttggatc cgtcctttcc cggtcaccgc
     1681 ctccaactca tgctggagga cgcccacgtt tccgtcgtgg tcacacaggc gcacctccgt
     1741 aaccatttac agggctatgg cggacaaatc tgtgacgtcg agaccttgtc gacgtccacc
     1801 gtgggcgcag cggaagagga gaacctcgca ctttccattt ccccggatca gctcgcctat
     1861 atcatctaca cctcgggttc gacgggacgg cccaaagggg tggccgtgac acaccacggt
     1921 ctggtgactt cccttcatgc gagacttcag tactatcctg atccggcatc ccgattcttg
     1981 ctgaccttct ccctggcctt tgacgggtcc gtgacgggca tattttggac gttgttgcag
     2041 ggaggggaac tggtcattcc atcggagacc gcccatcgcg acccaacgga gctggctgcg
     2101 ctcatcgagc gtcatcgcat ttcgcacgtc gtgtgggttc cgtctttgta ccatgctgtg
     2161 ctcggcgagg tgttgagcac tcagctggaa tcgctccgca tcatcgttac tgccggggaa
     2221 agcctgcccc ttgaactcgt gcgacgacat taccaactcc tgcctcacgc cgtgctgtac
     2281 aacgaatatg ggccgacgga agccacggtc tggtgcagcg tgtatcaaac gactcgtgag
     2341 gaagcagggg cccgagtacc gatcggcaaa cccatcgacc atatgcagct ctatgtgctg
     2401 gatgcaagtc tgcagcctat gcccatcggc ataccgggag aactctatat cgcgggagac
     2461 tgtctggctc gcggctatgt caaccatccg caattgaccc aagagcggtt tattgcgaat
     2521 ccctacgtgc ccggtaccag gctgtatcgg accggtgacc tggctcggta tcgcgccgac
     2581 ggcaatattg agtttctcgg acggacggac caacaggtca agctgcgtgg atatcggatc
     2641 gaactgggag aaatcgagta tgtgttgagt aacttcccgg gcgtgcatca agcggcggtg
     2701 ctccttcggc aggataaacc ggatcagcac cgactggtcg gctatatcgc cggcgagccg
     2761 tcgctcagag aaaggctcga ccaggttcgc agtcatctcg cagctcgatt gccgcactac
     2821 atggtgccat ccgccattct gtggctggaa acgatgcccc tcagcgccac tggaaaagtg
     2881 aatcgccatg ctcttcctgc tccggaagac acagccggtc gggcgacagc caggattgct
     2941 ccgaggaatc aggtcgaaga atccttggct gcgctgtgga aatccgtgct tcaagtcccg
     3001 gaggcaggca tccacgacca cttcttcgaa catggcggcc actcactggt ggctacccag
     3061 cttgtctcca gactacgcga gatttttgaa gtcgatgtct ccctacaggc gctttttgag
     3121 cgaccgacca ttgcggcatt ggccgaagaa gtaatacggt tgcgccaaag ggaaacggct
     3181 tccctgatgc caccgatcat tccagtgcct agagatcagc cgcttcccct ttcttattct
     3241 cagcaacgca tgtggctgat gcatcggctg gcgcctcaga gcacggcgta taacatgccg
     3301 ttcgcttcgc ggcagatggg acgactcaac aaaacagccc tccgaagtac gatcgatgcc
     3361 atctgtagtc ggcatgaggc atttagaacg acgttcatga tgaggggtga gggaccggtc
     3421 caaatggtcc acccctttcg ctctcctcat tgggtcgaag tgaatcttcg ggaactaccc
     3481 ctcgaacaac gacgactgca ggcggctcgg ctggtggagc aggaatcgaa tcagccgttc
     3541 gatctagaga aaggtcctct ggcaagattc ttcttgattg agatcgagcc ggaggatcat
     3601 gtgttggtgc tcacgatgca tcatatcatc ggagaccagt ggtcgttcgg cgtcatcggt
     3661 catgagtttg cattcttcta caatgcgttc tgccgaggag acgttccttc gccaaaaccg
     3721 atccgccttc agtatgccga ctatgccgtg tggcagcggc gttgtctgac cgacgaccgg
     3781 ctcagcacac aatcggatta ttggcaaaag aagctggccg gactgtccaa gctttcgttg
     3841 ccgaccgact acccacggcc ggtggtgcaa acatttaacg gctcacattg catgcttgag
     3901 ttgcccacgt cgttgatcga acgactgaag caattcagcg ctgaacacaa tgcgaccgtg
     3961 ttcatgaccc tgttggcctg tttccagatt ctcttgagtc gctattccgg acaaaccgat
     4021 gtggccgtag gttctcctat cgccaaccgg actcagtccg cggtcgagtc gatcatcggc
     4081 tcgtttgtca atacgctcgt cttgcgtacc gatctctccg gcgatccgac gttcatcggg
     4141 ttgatggtac gggtgcgtga aacggcgtta gaggcctatg ccaatcagga cttcccgttc
     4201 gataagttgg tcgagacgat gcactccgct cgcgaccaca gctccgcccc gctggtgcag
     4261 gtgctcttca atgtacccaa tgctcccatc ggggaaatca acgtccaagg gttgagttgg
     4321 gtgccgttcg aggtggagac gcaagcagcg caattcgact taagcttgac gatcgaaacg
     4381 gagttctccc gaagagccta cttgacgttc aataccgatc tcttcgagcc tcagaccgct
     4441 gaacgaatgt tgggccagta taagatcttg ctcatgaccg cattggccaa tccccagagc
     4501 aagctgtccg agcttccgac attgacggcg cccgagcggc aacagatgtt gttggattgg
     4561 aaccgcacgc aacgagatta tccacggtct gaatgttttc cccagctgtt tgaagcccag
     4621 gtcgagagga cgcctgatgc cgtggcgttg tcgatgggac gagagacgtt gcggtacggt
     4681 gagctcaatg cccgagctaa taagctggca cgctacctcc aaatgctggg cgctcgaccc
     4741 ggggtcgcgg tgggcatcgg tcttgaacga tccttggaaa tggtcactgc attgctggcc
     4801 gtgcttaaga ccggtgcggc ctacgtgcct cttgaccctg actatccacg ggatcgactt
     4861 cggtatatgt cggagaatgc ctcgctcatc attgtgctca catccgaatc gctggcggaa
     4921 cgatttgatg ttcacgtctg ccgcaggctg tgtctggatc gcgaagagaa gagcatcgcg
     4981 caagaggccg accacgatct tggcccgatc gcgacgcctc aagacctggc ctacatctta
     5041 tatacatcgg gatcgacagg gcagcccaag ggggtggaaa ttccacacca agcgctggtg
     5101 aacttcttgt gttcgatacg acaggagccg ggatgttccg ctcaagatgt catgctctcc
     5161 gtcacgacgc tctctttcga catcgccggg ttggagttat atgtgccgtt actggccggg
     5221 ggccgtgtcg agatcgccgg ccgcgctgtt gccatggacg ggcggcaact gttgactctc
     5281 tgtgaagctg ttcagccgac gatcatgcaa gcgactcctg ccacatggcg aatgctggtt
     5341 gaggcgggat ggcagggcag caccagactg acggtacttt gcggagggga accccttcct
     5401 cctgatcttg cggcatcact attagatcgt agcgttgctc tgtggaacat gtatggcccg
     5461 accgaaacca cgatctggtc cacaatcgaa agaatcgaac ggaccgctca ggagattacc
     5521 atcggaagac cgatcgccaa tacggaagtg tatatcctgg atcaattcct ccaaccggtt
     5581 cccgtcggag tttccggtga gctctatatc ggaggccatg ggttagcccg aggctatcgc
     5641 gggaggcctg atttgacaca ggagcggttc atccctcacc cattttctca agagcctctc
     5701 gcgcgactgt atcgaacggg agacttggca cggtatcgtc cggatggtcg catcgtgcac
     5761 ctcggtcgat tggatcatca agtcaagatc agagggtttc gaatcgaatt gggcgagatc
     5821 gaggcagcgc tgagccgtca tcaagctgtc cgtcaggccg tggtcctagc aagagacgat
     5881 cagcaaggtg tgaagcagtt agtggcgtat gtggtctgcc aggatagccc tgttccaaac
     5941 cagcaggagc ttcgctcgtt t
//
LOCUS       DATMTI010000242         4700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_179718, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000242 DATMTI010000000
VERSION     DATMTI010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179718"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..2197
                     /gene="dxs"
                     /locus_tag="VN039_04100"
     CDS             254..2197
                     /gene="dxs"
                     /locus_tag="VN039_04100"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWV45199.1"
                     /translation="MSILKTIHSPADLKRLSPDKFPTLCREIREQIIGAVSNVGGHLA
                     SNLGVVELTVALQYLLDTPADKIVWDTSNQSYTHKLLTGRREQFHTLRQYGGLSGFCK
                     REESEYDTFNAGHAGTGVSAAFGMVEARDQLKQKHKVVCVVGDGAMTAGMTLEGLHHA
                     GGLGKDFLVILNDNQMSISKNVGAISAYLSRTITGEFYGKMREETGQLLGKIPHIGSD
                     IQKLARRAEELAKGVILPGLLFEELGFQYSGPIDGHNFEHLLPTVENVLKMKGPVLLH
                     VITKKGLGYEPAMKNPVWFHACPPFVRSTGAPAKKAARPSYTAIAMDALVKLAREDKR
                     VVAITAAMCEGTGLTAFEKEFPDRLYDVGIAEQHAVTFAAGLAAQGMKPVVAMYATFL
                     QRAYDQVVHDVATQNLPVAFCIDRGGLVAEDGTTHHGAFDYAYLRHVPNMVVMAPKDE
                     NELQHMVKTCLEFNGPISVRYPRGVSLGVKMDPVPQALPVGKGELLKDGTAVAIIAIG
                     VSVWQAVEAAERLNDEGVSTAVVNGRFVKPLDHELIVDVAKRVRYVVTVEEGCKIGGF
                     GSAVLETLSEAGVTEVKTKVLGLPDWYIEQGPQDLLRERYGLTAEGIYQSVKELIGKA
                     PGVQSPFTGAALVGHPHGDEQGS"
     gene            2256..3440
                     /gene="ispG"
                     /locus_tag="VN039_04105"
                     /gene_synonym="gcpE"
     CDS             2256..3440
                     /gene="ispG"
                     /locus_tag="VN039_04105"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWV45200.1"
                     /translation="MHISRRKTRQISLGKVKVGGDAPVSVQSMCSTDTRDVAATVAQI
                     HQLEAAGCELIRVAVPDDEAARALPHIKSAMTVPLIADIHFDHRLALKAADIVDCVRI
                     NPGNIGAWWKVEEVIKAVNERGIPLRVGVNGGSLERPLLEKYGWPSPEALAESALNAV
                     HALEDVGFTNMKVSLKASDVHHAIDAYWLFAHQSDYPLHIGITEAGTAMTGAVKSSIG
                     LGYLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLNHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLVRKV
                     PMEELMDTLIVEVEQLAKEKEAESNGEASASVPSNGWESMESQSDHPSTLGKEIPVLP
                     LR"
     gene            3550..>4700
                     /locus_tag="VN039_04110"
     CDS             3550..>4700
                     /locus_tag="VN039_04110"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HWV45201.1"
                     /translation="MESKPAIHRISEFVGSSLFVRLVKVGLVIAAVVHGYIISFGRSF
                     HFRDIDIHREIGRRFLSGEYLYANDYCYMYLPTTGIYFAPLLILERNASLALRYAVAV
                     GCLVITIMLFHRMLRGTSHSSAWSRLLVGVGAGALTLQFILNDLDDGGPHLILLGILT
                     CAIYAIWVGRERLGAIILGFGITLKITPALFVLLFLWKRQWRLASYTVLATVLWIVLP
                     ILYMGPTSWWDHHTEWTRNAVLSVLDRQMEGRQENELQKANLSLRHTMLRYLVAYPPE
                     HRLRQVDQSYKPVLDLPSPLANVIVGAAGLTLLGLFAWSSRQPYQGAGDPAWARECAG
                     TLLLALLFSPITWDQHLVWMIPAAYVVVSAAARLNGELPRTGYIMLALYV"
BASE COUNT         1030 a         1236 c         1404 g         1030 t
ORIGIN      
        1 ctatttttgc cggcctcgat tttccctact aaccgaacca tacggtatcc acaccaatga
       61 gacaatcttg agagacaatc atgactcttt gtcatgaggt caagaagaga ggcaacccac
      121 caaggcatgt ttcataagtg cttgacttca tatggttcct gtgtgagact ccaccaaatt
      181 tggcacccga acgaccgggt cggtcgggtg tgatggccgc gttgctgacg ggggagaacg
      241 aaggagacca tctatgtcca tactcaagac gattcatagt cctgccgatc tgaagcggtt
      301 gtctcccgac aaatttccga cattgtgccg ggagattcgt gagcagatta tcggagcggt
      361 ctcgaacgtg ggcggacacc tggcctcgaa tttaggcgtc gtcgagctca cagtcgcctt
      421 gcaatatctc ttggatacac cggccgacaa aatcgtctgg gacaccagca accaatcata
      481 cacgcacaaa ctcctcactg gtcggcgcga acagtttcac acgcttcgcc aatacggcgg
      541 actgagcggg ttttgtaagc gggaagagag tgagtacgat acattcaacg ccggccatgc
      601 ggggaccggt gtgtcggcgg ccttcggtat ggtcgaagcc agagaccagt tgaagcaaaa
      661 gcataaagtc gtctgtgtcg tcggggacgg cgctatgacg gcgggaatga cgctggaagg
      721 gctgcatcat gccggaggac ttggaaaaga ttttctcgtc attttaaacg acaaccaaat
      781 gtcgatctcg aaaaacgttg gtgcgatttc cgcctatttg agtcggacca tcacgggcga
      841 gttctacggg aaaatgcgtg aagagaccgg tcagctattg ggcaagattc ctcacatcgg
      901 ctctgacata cagaagctcg cccgccgagc cgaagaactc gccaaaggcg tgattctacc
      961 gggattgctt ttcgaagaat tgggttttca atacagtgga cccatcgacg gccacaactt
     1021 tgagcatctt cttccgacgg ttgagaatgt gctgaagatg aaggggccgg tcctgcttca
     1081 tgtcatcacg aagaaggggc tcggctacga accggccatg aagaatccgg tatggttcca
     1141 tgcatgccct ccgttcgttc ggtcgaccgg cgcaccggcc aagaaagccg cgcgcccgtc
     1201 ctacacggcg atcgccatgg acgcgctcgt caaattggct cgtgaggaca agcgcgtcgt
     1261 ggccatcacg gctgcaatgt gcgaagggac ggggctgacc gcattcgaaa aggaattccc
     1321 ggatcgtctc tacgacgtcg gcatcgccga gcagcatgca gtgacgtttg cagcggggct
     1381 cgccgcgcag ggaatgaaac cggtcgtggc aatgtatgcg accttcctcc agcgggccta
     1441 tgaccaagtc gtgcatgatg tcgccaccca gaatcttccg gtggccttct gcatcgatcg
     1501 aggagggctc gtcgccgagg acgggacgac gcatcacggg gccttcgact atgcctatct
     1561 gcggcacgtt cccaatatgg tcgtcatggc tcccaaggat gaaaatgaac tgcagcatat
     1621 ggtgaaaacc tgcttggaat tcaacgggcc gatttcggtg cgatatccgc gcggggtgag
     1681 tctcggtgtg aagatggacc cggttccgca agccttgccg gtgggaaaag gcgagctcct
     1741 caaagatgga accgcagtcg ccatcattgc gatcggtgtc tcggtatggc aagctgttga
     1801 agcagccgaa cggctcaacg acgaaggcgt gtccacagcg gttgtgaatg gacgattcgt
     1861 caagccgctc gatcacgaac tgattgtcga tgtcgcgaag cgggtgcgct atgttgtgac
     1921 ggtggaggaa ggctgcaaga tcggtgggtt tggctcagcc gtgctcgaaa ccctttcgga
     1981 agccggggtg acggaggtga agacgaaggt cctgggcctg cccgattggt acatcgagca
     2041 ggggccacag gatctgttgc gggaacggta tggtttgacg gcggaaggga tctatcaaag
     2101 cgtgaaggaa ttgatcggca aagcacctgg cgtacaatcg ccgtttactg gtgcggcgct
     2161 ggtcggccat cctcatggcg acgaacaggg gagctgatgc agaagcgaaa cgcgaaaagt
     2221 aacagtaagg agtaagggct aagcggtttc agaccatgca tatttcaagg cgaaaaacac
     2281 gtcaaatttc tctcggtaag gtgaaggtcg gtggtgatgc tccggtgtcc gtgcaatcca
     2341 tgtgttcgac ggacacacgc gatgtggcgg cgacggtcgc acagattcat cagcttgaag
     2401 ccgccggctg cgaactcatc cgtgtggccg ttccggacga tgaggccgcc cgggccctcc
     2461 cgcacatcaa atcggcgatg acggtaccgc tgattgcgga tattcacttt gatcatcggc
     2521 ttgctttgaa ggcagcggac atcgtcgact gtgtccgcat caatcccggc aatatcggtg
     2581 cttggtggaa ggtggaagaa gtcattaagg ccgtgaatga gcgagggatt ccgcttcgag
     2641 tcggcgtcaa cggtgggtcg ctcgaacgac cgttgttgga gaagtacgga tggccttccc
     2701 ccgaggcgct ggccgagtcg gcgctcaatg ccgtgcatgc gcttgaggac gtgggcttta
     2761 ccaacatgaa ggtgtcgctc aaagcctcgg acgtgcatca tgccatcgat gcctactggc
     2821 tcttcgctca tcagtccgat tatccgcttc acatcgggat taccgaagca gggacggcca
     2881 tgaccggagc ggtaaagtct tccatcggcc ttgggtactt gctttcgcag ggaatcgggg
     2941 acacgttgcg cgtgtcgctg gctgctgacc cggtggaaga agtcaaagtc gggttcgaga
     3001 tcctgaaatc actcgaatta cgtcatcggg gcatcaatgt catcgcctgt ccgacgtgcg
     3061 gacgcgtcga aatcgacgtc gtccggatgg cgaacgagct ggagaagaaa ctcaaccaca
     3121 tcaagactcc attaaacgtg tcggtgctgg gttgtgtcgt caacggcatc ggagaaggga
     3181 aagaagccga tatcgggatt gccggcggcg agggcaaggg tattttgttc aagaaaggca
     3241 agcttgtccg aaaggtgccg atggaagaat tgatggacac gctcattgtg gaagtggaac
     3301 aactcgccaa ggaaaaagaa gcggaaagca acggcgaagc gagtgcctct gtcccttcaa
     3361 acgggtggga atcgatggag tctcagtcag atcatccctc gactcttgga aaagaaatcc
     3421 ccgtcttgcc cctcaggtaa cgggcgctgt taagaacata ttctcaccta cgtcttcaac
     3481 ccgtatcccc ttttgcaaaa gaaactgttg acgcactttc accggttctt gtatgtgtgc
     3541 cgctgagcca tggaatcaaa gcccgccatt caccggatca gtgagttcgt cggatcttcc
     3601 ctgtttgtga ggttggtgaa ggtcggtctc gtgattgcgg cggtggtgca cggctacatc
     3661 atctctttcg gacggtcctt tcacttccgt gacatcgaca tccaccgcga gatcggaagg
     3721 cgctttctct ccggcgaata tctctacgcc aacgactatt gctacatgta tttgccgacc
     3781 accgggatct atttcgctcc cttgctcatt ctagagagaa acgcaagctt ggcgctacgg
     3841 tatgccgtcg ccgtcggatg cttagtcatc accatcatgc tgttccatcg catgctgcgc
     3901 ggcacatcac actccagtgc gtggagccgg ctgttggtcg gcgtaggcgc gggagcgttg
     3961 acgctgcaat tcatcttgaa tgatctcgac gacggcgggc cgcacctgat tctcttgggc
     4021 attctcactt gcgcgatcta cgcaatctgg gtcggcagag aacgactcgg cgcgatcatt
     4081 ctgggattcg gcattacact gaaaatcact ccggcgctct tcgtgctgct gtttctgtgg
     4141 aagcggcagt ggcgattggc ctcatatacc gtgctcgcga ccgtcttgtg gatcgtactc
     4201 cctatcttat acatgggtcc cacaagttgg tgggatcatc acacggaatg gaccaggaac
     4261 gcggtgttat ccgtgcttga tcgacagatg gagggcaggc aagaaaatga acttcagaag
     4321 gccaaccttt cactccgcca taccatgctt cggtatctgg ttgcgtaccc gccggagcat
     4381 cggcttcggc aagtggacca gagttacaag ccggtgctgg atcttccgtc gccgcttgcg
     4441 aacgtgatcg tcggagcggc cgggctgact ctgctcggtc tgttcgcctg gtccagccgg
     4501 caaccgtatc aaggggcagg agatcccgca tgggcacgag agtgcgccgg tacgttgctc
     4561 ttggcattat tgttttcccc tattacctgg gatcagcatt tggtgtggat gataccggcg
     4621 gcctatgtcg tcgtgtcggc cgctgcaaga ttgaacgggg agttaccccg tacgggatac
     4681 atcatgctgg ccctgtacgt
//
LOCUS       DATMTI010000243         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_349341, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000243 DATMTI010000000
VERSION     DATMTI010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349341"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VN039_04115"
     CDS             complement(<1..1151)
                     /locus_tag="VN039_04115"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HWV45202.1"
                     /translation="MINRVILLVVDGFGVGTLPDGADYGDADANTLAHLAETVDGLSL
                     PNFEMLGLGHIASIKGVRAMAQPNGSFGRLEFASPGKDSVVGYWEIGGVIQKEAQTVC
                     GSGVPAKIVDVIEQLFGRKSIGRGISTMGVMLRRHSMEHMATGASILWTDGGNTCFLA
                     MHESIMAPVEFQQRCRETRKAVKDAGTLVRVVGQPVIGGHDLLRPQVGRKDFVIEPPG
                     VTMLDVLSRSGQIAMGVGKVYDLFSGRGLTKSFPAASGTAAFDEVIGMLNKVPRGLIC
                     ASLDLLSEDAVQSATAVQEFDRRLPDLFERLRLGDMVIVTGDHGRDFSLPGRTSTREY
                     VPVFVTGPKLAQGVDLGSRPTAADVGQTIVEALQAERLPVGESFLDALRP"
     gene            complement(1326..2003)
                     /gene="deoC"
                     /locus_tag="VN039_04120"
     CDS             complement(1326..2003)
                     /gene="deoC"
                     /locus_tag="VN039_04120"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWV45203.1"
                     /translation="MSGWNLPALIDHTVLRPDATKVEVLRLCEEAQANDFTVIFVPPC
                     YLDEAVAAVAGSGIRVGIPIGFPLGGHSTKVKVAEALDAVQRGATVLDMVLNISRLKS
                     ADHDYVRTDITEVVQSTAGVEHKVILETCYLTHQEKQTACHLIVEAGADYVKTSTGFG
                     AAGATVEDVRLLKEVVAGRVKVKASGGIRDWRTTLAMLEAGAERIGTSAGLKIIHEWR
                     ATRHQQE"
     gene            complement(2007..>2433)
                     /gene="mltG"
                     /locus_tag="VN039_04125"
     CDS             complement(2007..>2433)
                     /gene="mltG"
                     /locus_tag="VN039_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWV45204.1"
                     /translation="ETGVGDERPHISSVFHNRLKKHIPLQSDPTVIYGLPKFDGNLHK
                     KDLTHPSPYNTYRWAGLPPGPIANPGAQSIRAALYPVPSAYLYFVSKNDGTHQFSATL
                     IEHNRAVEKYQKRPFRRGNHSQTFMTPAGKQHHRKEGVS"
BASE COUNT          516 a          749 c          637 g          531 t
ORIGIN      
        1 ggcctgagcg catccaaaaa actctcaccg accggtaatc gctcggcttg aagtgcctcc
       61 acaatggtct gtcctacatc ggcagcggtc ggtctgcttc ccaaatcgac gccttgggcc
      121 agtttggggc cggtcacgaa gaccggaaca tactctcgcg tagatgtccg cccaggcaat
      181 gagaaatctc tcccatgatc gcccgtcaca atgaccatat cgccgagacg caacctctcg
      241 aacaaatcag gcagacggcg atcaaattcc tgtacggctg tcgccgactg tacggcatct
      301 tcggacagca gatccaagct ggcacagatg agaccgcgag gcaccttgtt caacatgccg
      361 atgacttcat caaaagccgc cgtccccgat gcggccggga aggatttcgt caaccctcgt
      421 ccactaaaga gatcgtagac ctttccgacg cccatcgcga tctggccgga tcggctcaag
      481 acatccaaca tcgtcacacc cggcggctcg atcacaaagt cctttcgtcc gacctgcggc
      541 cgaagcaagt catgcccgcc gatgaccggc tgcccgacga cgcgaacgag cgtccccgca
      601 tctttcacgg ctttccgggt ctcgcgacat cgttgttgga attccaccgg tgccatgatg
      661 gattcatgca tggccaaaaa gcaggtattg cctccatccg tccatagaat agacgcgccg
      721 gtggccatat gctccatact atgtcggcgg agcatcactc ccatggtcga gatacctcgg
      781 ccgatcgatt ttctgccgaa gagctgttca atgacgtcga cgatcttggc gggaacgcct
      841 gacccacaga ccgtctgggc ttctttctgg atcaccccgc ctatttccca gtatccgacg
      901 acagaatctt tacccggcga tgcgaattcc agtcggccaa aagaaccgtt cggctgggcc
      961 atggctcgta cgcctttgat cgaagcgata tgccccagcc cgagcatctc gaaattgggt
     1021 aaactcagtc catcgacggt ctcggccagg tgcgcgaggg tgttcgcatc ggcatcgccg
     1081 tagtcggcgc cgtccggcaa cgttcccacc ccaaacccat cgacaaccaa cagaatgact
     1141 cgattgatca tcagcgtact ataccaagtc gccgtagtcc gacatagatg cgtatgggac
     1201 cgacatgttt acctaggcag gcgaggaacg gctgttaggc cgcacgatca cgcaataggt
     1261 accggtgcaa atattccggg cgttgtgctg agcaaccgaa atccacgccc atactcagta
     1321 cggctttatt cttgttgatg tctggtcgcc cgccattcat ggatgatctt gaggccggca
     1381 ctggtcccga tcctttcggc tcccgcttcc agcatcgcga gcgtcgtcct ccaatcacga
     1441 atgccgcctg aggccttgac ctttacccgt cccgcgacga cttcctttaa caaccgcaca
     1501 tcttccaccg tcgcccccgc agctccgaag ccggttgacg tcttcacgta atcggccccg
     1561 gcttccacga tcaaatgaca tgccgtttgc ttctcctgat gcgtgagata acaggtttcc
     1621 aggatcacct tatgctcgac acccgcggta gactgaacaa cttcagtaat gtctgtccgc
     1681 acataatcat gatcggctga cttcagtcta ctgatattca gcaccatgtc caacaccgtt
     1741 gcgccccgct gcacggcatc gagggcttcg gccaccttga cctttgtgct atgccctccc
     1801 aacggaaatc cgatgggaat gccgacgcga atgccggagc cggccaccgc tgccaccgcc
     1861 tcatcgaggt agcatggtgg gacaaaaatg accgtgaaat cgttggcctg cgcttcttcg
     1921 cacaaccgaa gtacctcgac tttcgtcgca tccggcctca atacggtatg gtcgataaga
     1981 gcagggagat tccatcctga cattccttac gatactcctt ccttgcggtg gtgctgtttg
     2041 ccggcgggag tcataaaggt ctgcgagtga ttgcctcgtc ggaaggggcg cttctgatac
     2101 ttttccaccg ccctgttatg ttcgatgagc gtcgcggaaa attgatgtgt tccatcgttc
     2161 ttcgagacga agtagagata cgcagaaggc acgggataca aggcggcacg gatcgactgt
     2221 gccccggggt tggcgatcgg ccccggcggc agaccggccc accggtacgt attgtaggga
     2281 ctggggtggg tgagatcctt tttgtgaagg tttccatcga atttcggcag accgtagatc
     2341 accgtcgggt cgctctgcag gggaatatgt tttttcagcc gattatggaa tacggacgag
     2401 atgtggggac gttcgtctcc gacaccggtc tct
//
LOCUS       DATMTI010000244         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_730933, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000244 DATMTI010000000
VERSION     DATMTI010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730933"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VN039_04130"
     CDS             <1..1015
                     /locus_tag="VN039_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45205.1"
                     /translation="IHFTGVSNVAATFDFRFTAGATPALAGLASTQILPLISGNVAEN
                     PTISIAPIEGEDFTRRLLTPFNQSKLTLLLRQGVDIDLLLRLMAKELRLKGTEGAVAY
                     RNNPSDQVGYEMFRRVVLHLSTIQDHNSLHVEPLIIERSWTIPADSVTAEGFKALEQE
                     YQVTYNREDKTYTLLKQVEGGTLITNYDPDSLSKEERLLLQQKDEQVLLNDVTFDIRP
                     GHYGGDWPLRGDFRLRSFNAMLNFLGLSIAEEPEYHVDKDSRTPDVAENPVKTMDLIV
                     SPSAPTELDLAMKSHGQYYAVNTTGPQARWNREAFKLLSQLFQMTVTEVPRAGVPSIT
                     IAK"
     gene            complement(1054..1359)
                     /locus_tag="VN039_04135"
     CDS             complement(1054..1359)
                     /locus_tag="VN039_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45206.1"
                     /translation="MQEYAAYYGTACIIFSVYLLIKGFLAASRQRSEDRSTTLVPAYI
                     YGILASILLTVGLGFFRPSLPWWGYRLIFPGTTMLFPGIIYLVGRRPPKPRLDRVDA"
     gene            1594..1899
                     /locus_tag="VN039_04140"
     CDS             1594..1899
                     /locus_tag="VN039_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45207.1"
                     /translation="MKMLVIVFRESLVDQVHDLLNDYEVSAFSELYNVAGKGATGFTS
                     QFFLAPTTNRMILAAVSEQLAYRLIDAFTHFRLKQEAAEGDSTFPLHVFVLPCEQAV"
     gene            complement(2062..2430)
                     /locus_tag="VN039_04145"
     CDS             complement(2062..2430)
                     /locus_tag="VN039_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45208.1"
                     /translation="MRFILVSVVGSALALGTYGLAMAEETSSFESEFKQEMGSMASET
                     KDEMKAEQEQMQDSFKARKDQMKADMKANRAKMKSEMKAKRDALKAEKQEMKKAAKAK
                     KANAKKNAQKAAESLAPSTY"
     gene            complement(2920..5211)
                     /locus_tag="VN039_04150"
     CDS             complement(2920..5211)
                     /locus_tag="VN039_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWV45209.1"
                     /translation="MSGSLDFVAIEGLLAYGRALARRALERGLVAPPPTEASDDDVAR
                     VRAKMEQLRSFLKRSESGFATAEEYRSAREGFIDDRLVFFSAWNTLLAEGSLQPLLRA
                     TIGAVAKPTYRRPVAIVPRTQLTPTLAEGRIVLELGDDRFWLLPRDLGDRTLFFTMRH
                     GVSQVDSKTHRVGCRLPNQLDRERGVAKADAVGAALARMIGIVGQQLDFLHLTNYLDP
                     RTFLHCISRSPNTRQLYERVSAALLQGASASGPIAEAALESSDFGWVTGLEKSVEVEE
                     AARAFGVEPSAAKRLMKDPLYCYPDGNSFFDLYVDVIDGLHRLGSAQKGKVACLYTHS
                     STMRALMIYLDPRPFHEAFNEFSDYKESQDNVVLLTFEQGRLSGYSTAVGLSERERAV
                     RNTWVTVERTRKDHVTLKPRTLKRVVALVSGGDFAGAGAALKELHVTGQRMGLEVYFV
                     RHGYLGLANNWIERVTEEHTRGMGSHPSSPIGSSRFEEFKQEAIQHVAMRHLEPYMRD
                     GVLVVLGGDGSMRGARAIYEGFGVQVAGIPGSIDNNIEGTISLGFQSAVALADQSIES
                     LKATSAAMGSVFFVEVMGAGSGHLALACAYQARAEGLLVNEHPDPNAYIDDVILGTLK
                     RTLGVPNKSHLFVVAERTPHRHHKDGGVHGLVDYVADVITRWPERQARPGQYPLTLAT
                     KATILGHTLRGARPIPEDKAIAQHLAHEMVHRLIESPEDIVGCLLAYRERGSIGPIPL
                     HAVTPKQFDWDVFSRMHGNARLT"
BASE COUNT         1201 a         1535 c         1443 g         1174 t
ORIGIN      
        1 cattcacttt accggcgtct ccaacgtggc ggcgaccttc gattttcgtt tcacggccgg
       61 cgccactccg gctctcgctg gtttagccag tacgcagatc ctgcccctca tcagcggaaa
      121 tgtagctgag aacccgacca tcagtatcgc tccgatcgaa ggggaggact ttaccaggcg
      181 cttactcact ccattcaacc aatccaaact gacactgctc ctacgacagg gcgtcgatat
      241 cgatctcctc ctccgactga tggccaaaga gcttcgattg aaaggaacag aaggagctgt
      301 cgcctatcgg aataatccgt ccgaccaggt cggctatgag atgttccggc gtgtggttct
      361 ccacctctcg accattcaag accacaatag cctccatgtc gaaccattga tcatcgaacg
      421 aagttggacc atccccgccg attcggtgac ggcggaagga ttcaaagcac tcgaacagga
      481 ataccaggtg acgtataaca gggaagacaa gacatacaca ttgctgaaac aggttgaagg
      541 cggcacactg attaccaatt acgaccccga ttcgctatcc aaggaagagc ggctcctctt
      601 gcagcagaag gatgaacagg tgcttctcaa tgacgtgacg tttgatattc gcccgggaca
      661 ttatggcggt gactggccgc tgagaggcga ctttcgattg cgcagcttca acgccatgtt
      721 gaactttctg gggctctcca tcgctgagga gccggagtac catgtcgaca aagattctcg
      781 tactcccgac gtggcggaga atccggtcaa gacgatggac ctgatcgtgt caccgtcagc
      841 gccgacggaa ctggatcttg cgatgaaatc ccatgggcag tattacgccg tcaacacgac
      901 cgggccacag gcccgctgga accgtgaagc attcaagctc ttgtcacaat tatttcagat
      961 gaccgtcacc gaggtcccac gcgccggcgt gccaagcatt accatcgcca aatagggcgg
     1021 gatatccagc cgtctataat gagtggcgcc cggctatgcg tcgacgcgat cgaggcgagg
     1081 cttcggaggt cgccgaccga cgagatagat gatgccggga aagagcatgg tcgtaccggg
     1141 gaaaatcaat cgatagcccc accagggcaa gctcggccga aaaaatccca aaccaacggt
     1201 cagcagaatc gacgccagga ttccatagat ataggccggg accagcgtcg tagaacgatc
     1261 ttcgcttcgc tgccttgacg cggcaaggaa gcctttaatg aggagataga cggaaaagat
     1321 gatacaggca gtaccgtaat acgctgcgta ttcttgcata aaaccgtcct ggttgatacg
     1381 cccacgaagc cgttggtaga taatttatag cgtatcgtct ctgctcttgt cgagccgggc
     1441 cttaggttcc tgtagaccca tcagtccatc tacctgatgc aagaagagcc ctttgacctt
     1501 gtatttgccg atatctcccc cgagaatgga tcgaacaccg gctcgtatcc gcaaggttgg
     1561 tgacagtcca tcccgtgcac aggagtgcgg cctatgaaga tgttagtgat cgtctttcgt
     1621 gaatcgctcg tggaccaggt gcatgatttg ctgaacgact acgaggtgag cgccttcagc
     1681 gagctgtaca atgttgccgg aaaaggagcg accggcttca ccagccagtt ctttctggcc
     1741 ccgacgacca accgaatgat tcttgcagcg gtctctgagc agttggctta ccgtctcatc
     1801 gatgccttta cacactttcg attgaagcag gaggccgccg agggcgacag caccttcccg
     1861 ctccatgtct ttgtgctgcc ctgcgaacaa gccgtgtaac gagatccgat aaccatccac
     1921 agtcagcggc gcgattcagc ctgaaagagt cctgagatgc ggatccacac gtgagtccaa
     1981 acacgacggc ctccggaaat aatccccgga ggccgttgtt gaaagctgca tgtttcacgg
     2041 agtcgttcag acaccgttgg gtcagtaggt gctcggcgcc agtgattcgg ccgctttttg
     2101 ggcattcttc ttggcgttgg ctttctttgc cttcgccgct tttttcattt cctgcttctc
     2161 cgccttcagg gcatctcgct tggccttcat ctccgatttc atctttgctc tgttggcctt
     2221 catgtcggcc ttcatctggt ccttcctagc cttgaagcta tcctgcatct gctcctgctc
     2281 ggctttcatc tcatccttgg tttcgctggc catcgagccc atctcctgtt tgaactctga
     2341 ctcgaagctt gaggtttctt ccgccatggc caaaccataa gttccaagtg cgagtgcgga
     2401 cccaactacc gatacgagga tgaatcgcat ggataaacct ccctaaaaag attgtgtcaa
     2461 cgtgagcggc gccggaaacc ggtgtccggt ctagagctgt cctgaggttg tgtttccagc
     2521 aatggagcaa agaaaccacg gcctccagta gattgttccc tggaggccgc acaagagccc
     2581 gcactcaatg gagtacgctc ggtgctttca tatggagaca agcgctatgg cctgatctgc
     2641 cacagcaaat acaccaaccc cacagtcact gctgatacca aaacgaaatg catggactca
     2701 cctccctatg tcgtaccctg tctgacgccg gaaaccggcg ttcgatttac aggtatcgcg
     2761 agatgaattt tcaggcaact tatttcaaga ttttggatgt cagatatgcg agagctggcg
     2821 atgtggcgcg ctttgaaacc ttgctacggc cggcgcgact gttttcgcta cgatcaacgt
     2881 cctatcgcgt tccggaagca aataggtggc ggctgagagt tatgtgaggc gggcattgcc
     2941 atgcatccga ctgaaaacgt cccagtcgaa ctgcttgggt gtcacggcat ggagtggaat
     3001 cggtccgatc gatccccgtt cacggtaggc taagagacat ccgacaatgt cctcgggtga
     3061 ctcgatcagt cggtgcacca tctcatgggc aagatgctga gcaatggcct tgtcctccgg
     3121 tatcggccgt gcgcctcgga gtgtgtggcc aaggatggtc gctttggtgg caagtgtgag
     3181 cggatactga ccaggccgcg cttgccgttc cggccatcga gtgatcacgt cggcaacata
     3241 atcgacgaga ccatgaactc caccatcctt gtgatgccgg tgcggcgtac gttcggcaac
     3301 gacgaacaag tggctcttat tcggtacccc caatgtccgt ttgagagtcc cgaggatcac
     3361 gtcatcgata taagcattcg ggtcgggatg ttcattgacc aacaacccct cggctctcgc
     3421 ctgataggcg caggccaacg ccagatgacc tgatccggca cccatgacct ccacaaaaaa
     3481 cacactgccc atcgccgcgc tggtggcttt aagcgattca atcgactgat ccgccaatgc
     3541 caccgcggac tgaaatccaa gagagattgt tccttcaata ttattgtcga tactgcccgg
     3601 aatgccggcc acctgcacgc cgaatccttc gtaaattgcc cgcgcacccc gcatactgcc
     3661 gtcgcccccc aacacaacca atacgccgtc acgcatgtac ggttccaggt gccgcatggc
     3721 cacgtgctgg attgcctctt gtttgaactc ctcaaaacgg ctgctcccga tgggactgct
     3781 gggatggctg cccatgccgc gggtgtgctc ttcggtgacc cgttcgatcc aattgttggc
     3841 caaccccaga tagccgtggc ggacgaaata gacctccaat cccatgcgtt ggccggtcac
     3901 atgcaattct tttaacgccg cgcctgcccc ggcaaaatca cctccggaga cgagagcaac
     3961 gactcgtttg agcgttcgag gtttgagagt gacgtgatct tttcttgtgc gctccactgt
     4021 cacccaggtg ttgcgcacag cccgctcccg ttcggacagc ccgaccgccg ttgaatagcc
     4081 ggataggcga ccttgttcga acgtgagcag cacgacattg tcttggcttt ccttgtagtc
     4141 gctgaactca ttgaaggctt catggaacgg gcgagggtcc aaataaatca tcaaggcccg
     4201 catggtggag ctgtgggtat agaggcaagc cactttgccc ttttgcgcgg agcctaatcg
     4261 gtggagaccg tcgatgacgt cgacatagag atcgaagaaa gaattgccgt ccggataaca
     4321 atacagcgga tctttcatca accgcttggc cgcggaaggt tccaccccga acgcccgcgc
     4381 ggcttcttcg acttccaccg acttctccaa tcccgtgacc caaccgaaat ccgatgattc
     4441 gagagccgcc tcggcgattg gtccggacgc ggaggctccc tgcaacaacg ctgcggacac
     4501 ccgttcgtag agttgtctgg tattcgggct acggctgatg caatgaagga acgtacgtgg
     4561 atcgaggtag ttggtgagat gcaaaaaatc gagctgttgt cccacaatgc cgatcatgcg
     4621 ggcgagcgcc gcccctactg cgtcggcttt ggcaacccct cgttcgcggt ccaattgatt
     4681 cggcaatcgg catccgacac gatgggtctt actgtccacc tgtgacacgc cgtggcgcat
     4741 cgtgaagaag agtgttcggt cacccagatc gcgaggtaac aaccaaaaac ggtcgtcgcc
     4801 cagttccagg acgatgcgac cttccgccag cgtaggcgtg agctgcgtac gaggcacgat
     4861 ggccacagga cgtcgatagg tcggtttggc gactgctccg atcgtggccc ggagtaacgg
     4921 ttgcagcgag ccttcggcaa ggagtgtgtt ccaagcggaa aagaacacca gccggtcatc
     4981 gatgaaacct tcgcgagccg atcgatactc ttcagcggtt gcaaagccgg actcggagcg
     5041 tttgaggaac gagcggagtt gctccatctt ggcgcggact cgagcaacat catcgtccga
     5101 cgcttctgtc gggggaggtg ccacgagtcc acgctccaac gctcgtcgag cgagggcgcg
     5161 accataggcc aggagtcctt caatggcgac gaaatccagt gagccgctca taacaggcgt
     5221 caataagatc gtgttctacc aagcgttatt ctgagtgcaa agaatgctct ctataaccta
     5281 cttgaagcgt gatgatccat gcaatgttag caatcctaaa ccgatgcaca gaacgtagca
     5341 agaggtgcaa ggg
//
LOCUS       DATMTI010000245         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_51768, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000245 DATMTI010000000
VERSION     DATMTI010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51768"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VN039_04155"
     CDS             <1..892
                     /locus_tag="VN039_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV45210.1"
                     /translation="WTGDMKGDKRIAYSSMAPREKDACFSIEDGLNPRRFDGKRALLL
                     PLTTVCVGLPAAVDEIGWSGYEARGDHARSVGNWPEAEKAYGVAVGMLDRTADKEINQ
                     DLATLLNKLGAMRFRQNDFTGAETVFRRALTIYTLARGADDLHVADTLDLVASALFEQ
                     PQDRALAGPLFYRAWAIRDGTLGPNHPAIADSLHHLAVSLYSDNVSLAIPLFLRSKEI
                     REKAFGHDHPLVANSLNAMARLYERHKRLDLAIPLYQAALTIQEKVFGPNASETLQTR
                     SSLDMVHGWKDHLHENLNGR"
     gene            complement(1132..1659)
                     /locus_tag="VN039_04160"
     CDS             complement(1132..1659)
                     /locus_tag="VN039_04160"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWV45211.1"
                     /translation="MKSVLTVAVTITAGIVLSGCVLTKKMMPGTMSSSEIFGVLDTIN
                     RSEIDAGKLAKEKATAPEVRSFASRMVSEHERMMEEMNELARRMDVQPQKPALAFTLN
                     ASHQDAMEELRDKSGSDFDKAYIEYQVKMHEQAIDLVRNGADEADNFSLKKHLRETRP
                     ELQNHLASAESIERE"
     gene            2241..2504
                     /locus_tag="VN039_04165"
     CDS             2241..2504
                     /locus_tag="VN039_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45212.1"
                     /translation="MQAEQAATRDSHVVFGEVVRVDGSTYVVKEKGGKEVSVKTDEKT
                     EQSPIKKGDHISANVDKNNHALWIRGNRGTDRRTEHVSGDCNP"
     gene            2520..2798
                     /locus_tag="VN039_04170"
     CDS             2520..2798
                     /locus_tag="VN039_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45213.1"
                     /translation="MGFPDGIDGNDLPARGFGQVNRFNVEVMEDDVHALRRISKSWFE
                     VSGVAKGYMAKRVLLATGLTHLPPNIPGVHDHCRRLEGDLRNHDISVA"
     gene            2779..4335
                     /locus_tag="VN039_04175"
     CDS             2779..4335
                     /locus_tag="VN039_04175"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWV45214.1"
                     /translation="MISQWLKWTVVSLAVLMVVLSTAAFFADEPLRRYVERTVNEAVE
                     GYSFKVGTLALHPLALSVDLGDVVVRQEAHPDPPLISIPEVVVDARFAPLLNGKAKGD
                     IRLKNPIISVTQQQINTASAEADQGTEQTVSWQDRIRGMIPFEATLFIKNGEVRYVGQ
                     RNAEPIRVTALDIEARNLTNRPQAAQAYPSELVMRSRLFDAGYLEFNGLADLLNKPLP
                     MVDASVKVSGLRIADLMPIVGAYHLVVNRGTFSATGRIMHEKQMVLSLESFVLEGAKI
                     AYVYQEETKSKQRRQLERGAERAAKAHRDPSFVFKVGHGEIFNSEVEFVNRSTDPAYR
                     VFLTDVNADIENFSNRLEEGTGVVKITGKFMGSGPAVVTGTFRPEKPRPDFNLAVKII
                     KTDVTTLNHVLRAYGDADTHHGKFALFSELTVKNNRIDGYVKPLFRDVDVYDPERDQD
                     KTLSHKIYRAIVDGVMSLLENIPRDEVATKGDVSGPVENPQSSTWQIIAKLVQNAFFN
                     AILPGFEERA"
BASE COUNT         1031 a         1153 c         1252 g          979 t
ORIGIN      
        1 gtggacaggc gatatgaagg gcgataagcg catcgcgtat agctcgatgg ctccaagaga
       61 aaaggatgcg tgcttttcga tagaggacgg attgaatccg cgaagatttg acggcaaacg
      121 cgccttgctc ttgccgttga cgacagtgtg tgtcggtttg cccgccgccg tcgatgaaat
      181 cggctggtcg gggtatgaag cacgagggga ccatgcgaga tcggtcggga actggccgga
      241 ggcggaaaag gcatacgggg tggctgtcgg gatgctggat cggaccgccg ataaggaaat
      301 caaccaagac ctggcgactc ttctcaataa attgggcgcc atgcggttca ggcaaaatga
      361 cttcaccggc gcggaaacgg tctttcgtcg ggcattgacg atctacacgt tagcccgtgg
      421 agccgatgat cttcatgtgg ccgatacctt ggacctggtc gcgagcgcat tattcgaaca
      481 gccacaagac cgcgcattag ccggcccatt attctatcgt gcctgggcga ttcgcgatgg
      541 gacgttgggg ccgaaccatc ccgccatcgc ggacagcctc caccatttgg ccgtcagttt
      601 atactccgac aacgtatcac ttgcgatccc tctgttcctc cgttcgaagg agatccgaga
      661 aaaggccttt ggccatgacc atccgttggt tgcaaactcg ctcaacgcga tggctcggct
      721 gtatgaaagg cataagcgcc tggatctcgc catcccgctc tatcaagccg cgttgacgat
      781 acaggaaaag gtattcggac cgaacgcctc cgagacgctg cagacccgtt ccagtttgga
      841 tatggttcat ggctggaagg accatctcca cgagaatttg aacggccggt aaatctttct
      901 caggctgccg tcgccctggc gacagccggc ttcaactcca ctttcatcca tctcggccgg
      961 cggctgtcaa atacccgaca ggcatcatgg ccgtactcag cggttccact cgcgtcaaga
     1021 cctcggctgg atcgcgaccg aaaggagttg cagatgcgtg ccatgcgcag accatgcact
     1081 cgccaagccg cctaggcacc atcgttcaac ccccatcgac atcgcaggct atcactcacg
     1141 ctcgatggat tcagccgatg ccaaatggtt ttgcagttcc ggccttgtct cgcgcagatg
     1201 cttcttgagg ctgaagttgt cggcctcgtc ggccccattt cttaccaggt caattgcttg
     1261 ttcgtgcatc ttgacctgat actcaatgta cgccttatcg aagtccgagc cggatttgtc
     1321 ccggagttcc tccatcgcgt cctgatgaga agcgttcaac gtaaaagcca gcgccggctt
     1381 ttgcggctgt acgtccatgc gtcgggccaa ctcgttcatt tcctccatca tcctctcgtg
     1441 ttcactgacc atgcgagagg caaacgagcg aacttccggg gcggtggcct tctccttagc
     1501 aagcttaccg gcatcgattt cgctgcgatt aatcgtatcg agcacgccaa aaatttcgga
     1561 actcgacatg gtgccaggca tcattttttt ggttaacacg cagcctgaga gcacgatccc
     1621 ggcggtgatg gtaacggcga cagtcaatac tgatttcatg acaacctctc ttcttatgag
     1681 tcgttgtcgg ttaaggaatg cataccaatc atgtccatgc aagacaagag cctactaggc
     1741 ttacgcgggt gaatcgttct gctctgttgt tgtgatggca ttcggaggga ctcaggttga
     1801 ggtttgggtt gagtggaccc ggaggcatcc tcacctcatc cttaacccca ggctcaaact
     1861 ggtagtcagg gaggccaagt gtcctcaagt cggacgaact gtctggccat agccaacagt
     1921 tccataacga ttggtgacgc ggaaaactgg tacgagggga atcaccagtt ggttcgcgac
     1981 agacttgagc cgatgattga tcacaaggcg tgtcgatcgt cggtgattca tgtgcgaagc
     2041 ccgtgcaaag gaatgcgcat actgcgtgag accgccctca cttaaccagg aggcatatga
     2101 tgacttcaaa agctgctcga ttgttgtcag gcggaatgtt attgctcgga ctatccacta
     2161 tgccgcaggt cggtgatgtc gcgtccgcgg cagataagct aaaggaaacc ccttgtgaag
     2221 tatcagaagg tacaaatgct atgcaggccg aacaagccgc gacgcgtgac agtcatgtcg
     2281 tgttcggtga agtcgtacgc gtggacggct cgacttacgt ggttaaggaa aaaggtggca
     2341 aagaagtgag cgtgaagacc gacgagaaga cggaacaatc tccgatcaag aaaggagatc
     2401 acatttcggc gaatgtagat aagaacaatc atgccctgtg gattcgaggg aacagaggga
     2461 cggatcgtcg aactgagcat gtctcgggtg actgcaatcc gtgaccatgt gcagaactat
     2521 tggggttccc cgacggcatc gacggaaatg atttgccggc gcgggggttt gggcaagtca
     2581 accgcttcaa tgtcgaagtt atggaagatg atgttcatgc attgagacgg atcagcaaga
     2641 gttggttcga agtgtcgggt gtcgcgaaag gctatatggc caaacgggta ttgttggcga
     2701 cggggctgac gcacctccca cccaacattc caggagtgca tgatcattgc cgccggttag
     2761 aaggcgactt gcgcaatcat gatatctcag tggcttaaat ggacagtcgt cagtctcgcc
     2821 gttctgatgg tagttctctc aaccgccgcc ttctttgcgg atgaacctct tcgccggtat
     2881 gtggagcgaa ccgtcaacga agcggtggag ggatacagct tcaaggtcgg cacactcgcc
     2941 ttacatccgc tcgccctatc ggtggacctg ggagatgtgg tggtgcgaca agaggctcac
     3001 ccggatcctc cgctgatcag tattccagaa gtcgtcgtcg atgcgcggtt cgcgcctctg
     3061 ttgaacggaa aggcgaaggg cgacattcgt ctcaaaaacc cgattatttc cgtcacacag
     3121 caacaaatta acactgcctc ggcagaggcg gaccaaggaa ctgagcagac ggtctcttgg
     3181 caagatcgga ttcgaggcat gatccctttc gaagcgacac ttttcatcaa gaacggggag
     3241 gtgaggtatg tcgggcagcg aaatgccgag ccaatccgag ttacggctct ggatattgag
     3301 gcgcgcaatc ttacgaatcg tccccaagcc gcgcaagcct atccctctga actagttatg
     3361 cgcagtaggt tgttcgatgc cgggtatctg gagttcaacg gtctggcgga tctactgaac
     3421 aagccgcttc ccatggtcga tgcgtccgtt aaggtgagcg gactgcgcat cgccgatttg
     3481 atgcccattg tcggagccta tcacttggtc gtgaatagag gcactttcag tgcgacagga
     3541 cgaatcatgc atgagaagca gatggttttg tcactcgaat cgtttgtgct ggaaggggca
     3601 aagatcgcct atgtatatca ggaagagacc aaatccaagc agcgtcgtca acttgagcgt
     3661 ggagcggaac gagcggcaaa ggctcaccgg gatccgtcgt ttgtgttcaa agtcgggcat
     3721 ggggaaatat tcaacagcga ggtggaattc gtcaatcgct cgaccgatcc ggcctatcgt
     3781 gtcttcttga cggacgtgaa tgccgacata gaaaacttca gcaatcgact cgaggaaggc
     3841 accggcgtcg tgaagatcac cggcaagttc atgggcagcg gtcccgcggt cgtcaccggc
     3901 acgtttcggc cggaaaaacc gagaccggat ttcaatctgg ccgtgaaaat catcaagacc
     3961 gacgtgacca cgctcaacca tgtcttgcga gcctacggcg atgccgatac ccaccatggc
     4021 aagtttgcgc tgttcagcga actgacggta aaaaataatc gtattgacgg gtacgtcaaa
     4081 ccgctgttcc gggatgtcga tgtctatgat ccggaaaggg accaagacaa gactctatct
     4141 cacaaaatct atagggcgat cgtcgacggt gtgatgtcgt tattggagaa cattccgcgc
     4201 gacgaagttg cgacaaaagg ggatgtgtcg gggcctgtcg agaatcctca gtccagtaca
     4261 tggcagataa tcgcgaaact ggttcagaat gcgtttttca atgccatcct tccgggattc
     4321 gaggagcgcg catgaaccat acttctgccg aatcatcgct caccagccaa tcgcccgcgt
     4381 acaatccctg gaaacttggc gggctttcct actat
//
LOCUS       DATMTI010000246         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_519135, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000246 DATMTI010000000
VERSION     DATMTI010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519135"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VN039_04180"
     CDS             <1..227
                     /locus_tag="VN039_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45215.1"
                     /translation="IPKHLLKGIQLAFADTMDDVMKIALRRSAKTSPASRKPSTPPAP
                     TRIRPLRPGKGRRSHELPATVMANRAPIRS"
     gene            237..1280
                     /gene="thiL"
                     /locus_tag="VN039_04185"
     CDS             237..1280
                     /gene="thiL"
                     /locus_tag="VN039_04185"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWV45216.1"
                     /translation="MAPRPANAPLQEFALIRSLEGRFARHAQGLVQGIGDDAAVIETS
                     AKTWWHLTTDLLAEGIHFDLKSVTPESVGYRAAMANLSDIAAMGAVPRYLLISLAVPR
                     IVKRSHIDALYRGLMKACRFYDVALIGGDTSASKTGLFLSITLVGTTSGRRALFRHGA
                     EAGDHIYVSGTLGDSLAGLNLLTNSTTPQPLEKRKSEFSRSHRRFLVRRHSHPTARVA
                     EGRWLNERQLASAAIDLSDGLSGDLRHLCEASRVGAEVELEKLPISPACRAYARTIGI
                     SPIRLALTGGEDYELLFTASSRDSNTIDRQAETRGYRMTRIGTIRPHRFGIQMTSDGR
                     TQPMPATSYEHFR"
     gene            1280..>1623
                     /locus_tag="VN039_04190"
     CDS             1280..>1623
                     /locus_tag="VN039_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HWV45217.1"
                     /translation="MSDMNHQPSTRGARSFRALLRQMLHLQESPQRTALAFALGVFIA
                     FSPAYGLHTAMVVLCTWLFELNFLALFTGALVNNPWTIIPILGATYWTGALLLGRTDM
                     PNFDWQDLSFSG"
BASE COUNT          330 a          529 c          429 g          335 t
ORIGIN      
        1 aaatccccaa acatctcctt aaaggcattc aattggcgtt cgccgatact atggacgacg
       61 tcatgaagat cgccctccgc cgaagcgcca agacttcgcc tgcctctagg aaaccatcga
      121 cccctccggc cccgacgcgc atccgccccc tgcgaccagg caaaggccga cggtcgcatg
      181 aacttcccgc cacggtgatg gcaaaccgag cgcccatacg atcataggat agtgccgtgg
      241 cccctcgtcc ggccaacgcc ccgcttcaag aatttgctct gatccgatcg ctcgaaggtc
      301 ggtttgcccg tcatgcgcaa gggctcgttc aaggcatcgg cgacgatgca gccgtgatcg
      361 agacatccgc caaaacctgg tggcatctca ccacggacct gcttgccgaa ggaatccact
      421 ttgatctgaa atcggtaacg cctgagtcgg tcggctaccg agccgccatg gccaacctca
      481 gcgacattgc cgcgatgggc gccgtccctc gctatctgct gatttcgctc gccgtcccga
      541 ggatagtgaa gcggtcacac atcgatgcgc tgtaccgcgg cttgatgaag gcttgccgtt
      601 tttatgacgt ggcactcatc ggtggagaca cctcggcgtc caagactgga ctctttctca
      661 gcattaccct ggtcggtacc acgagcggac ggcgggcgct ttttcggcac ggtgccgaag
      721 cgggagatca catctatgtc tcgggtactc tcggagactc cctggcaggc ctcaacctct
      781 tgacgaacag cacaaccccg caacccttgg aaaagaggaa gagcgaattc tcacgctccc
      841 atcggcgatt tcttgttcgc cgccattccc accctaccgc gagggtcgcc gaaggacggt
      901 ggctcaacga gaggcaactc gcctccgccg ccatcgatct gtccgacggc ctctccggcg
      961 atcttcggca cctgtgcgaa gcaagtcgag tcggcgcaga ggtggaactt gaaaagctac
     1021 ccatctcacc ggcctgtcga gcctatgccc ggacgatcgg catctcgccg attcgactcg
     1081 ccctcaccgg tggcgaagac tatgaacttc tgttcaccgc ttcttcgcgc gacagcaata
     1141 ctattgatcg gcaagcagaa acacgcggct atcgcatgac ccgtatcgga acaattcgcc
     1201 cgcatcggtt cggaattcag atgacgtctg acggtcggac gcagccgatg cccgctacca
     1261 gttacgagca tttccgctga tgtccgacat gaatcaccag ccctcaacgc gaggggcccg
     1321 atcattccgg gcactcttac gccaaatgct ccacttacag gagtcaccgc aacgcaccgc
     1381 gctggcattt gcgctgggcg tcttcatcgc gttctcacca gcctatggtc tgcacacggc
     1441 gatggtggtg ctgtgcacct ggctgttcga actcaatttc ttggccttgt tcaccggtgc
     1501 cttagtcaac aatccctgga ccatcattcc gatcttaggc gcaacctact ggaccggtgc
     1561 attgctcttg ggacggaccg atatgccgaa ttttgattgg caggatctga gtttcagcgg
     1621 tat
//
LOCUS       DATMTI010000247         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_179919, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000247 DATMTI010000000
VERSION     DATMTI010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179919"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..>1606
                     /gene="lptD"
                     /locus_tag="VN039_04195"
     CDS             136..>1606
                     /gene="lptD"
                     /locus_tag="VN039_04195"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HWV45218.1"
                     /translation="MTLGDTLGFKSAWFCVLDVPTIPLPAFSYPMIKRRTGFLMPIPS
                     YDNRFGFHVKQGFFWAINPSHDLTVTPSYYSDLGYGSDFEYRYALDWRSRGKWYLSYL
                     QQTQLPNVAGVTDTGQNAEKARASLVGSHTQYITDTLLLRANANLVTDPNYFQQLSNS
                     GVLRALPSTESNLLMTQRLPYGNLYLVGIYLQPLQAGGKDTFQRLPEAGYSLPYTSLF
                     NSPLLFGMDGNYVNFYRDQGFTLNRIDMVPGISTDVIHLGHVLGIRPQAKFREVYYTR
                     GAQSDEGQHRETFWLGVETTSKVTRRFTLADGNSLLHTVEPSVMYEYVPSTDQSKLTQ
                     IDQVDDLPKKNLLTYMLRSRVLESGGKNAFNWLDLTLAQSYHVGDVQTLARDFTPGVA
                     PFLGSLTQPLQPATTPIQGKKFSDIWMRAVIGNNLPTLMTPAWLDAPVGGRAAQAWAL
                     RPPINRYLTVDAFFDPYQPGVSQFNTDLRFQEGTNWYFEV"
BASE COUNT          359 a          426 c          438 g          383 t
ORIGIN      
        1 aactcattaa tatccggtcg cctcctgcag agattttccg aataccatta ccgagtcaaa
       61 gagggcagtt tcacgaattg tgatgcgcaa gacggagaag tgccggcctg gcgctttcgg
      121 tttaaggatt tggatatgac cctcggggat acgttgggat tcaagagcgc atggttctgc
      181 gtcttagacg tgcccacgat tccattaccc gcgttctcct atccgatgat caaacggcga
      241 accggatttc tgatgccgat cccgagctat gacaatcgat tcggattcca cgtgaaacag
      301 ggcttttttt gggcgatcaa tccgagtcat gacctgacgg tgacgccgtc gtattacagt
      361 gacctggggt atgggtccga ttttgagtat cggtatgcct tggactggcg atctcgtggc
      421 aagtggtatc tgagttatct acaacagacc caacttccca atgttgccgg agtgaccgac
      481 accgggcaaa atgccgagaa ggcacgcgcg tcgctcgtcg ggagccatac gcaatatatc
      541 accgatacgt tgctgctccg tgccaacgcc aatttggtca cagatcccaa ctatttccaa
      601 caactgagca attcgggggt actccgagcc ttaccgagca cggaatcaaa tctcttgatg
      661 actcagcgct tgccctacgg caacctctat ctcgtgggca tctatctgca accgttacaa
      721 gccgggggaa aggatacgtt ccaacgtctt ccggaggccg gctatagcct tccctatacc
      781 tcgctgttca attcgcctct gctgtttggt atggatggga actacgtgaa tttctatcga
      841 gatcaaggat ttacgcttaa tcggatcgat atggttccgg gtatttcgac ggacgtgatt
      901 catctcgggc atgtccttgg gattcgaccg caagcgaagt tccgtgaggt ctattacacc
      961 agaggggcgc aatcggatga agggcagcat cgagagactt tttggttggg ggtggagacc
     1021 acatcgaagg tgacacgccg gttcaccctc gctgacggga acagtctttt gcatacagta
     1081 gaaccctcgg tcatgtacga atatgtcccg tcgacggatc aatcgaagtt gacgcaaatc
     1141 gaccaagtcg atgacctacc aaagaagaat ctgctgacct atatgttgcg cagccgcgtg
     1201 ttggaatcag gcgggaaaaa tgccttcaac tggctcgatc tcacgttggc tcagagctat
     1261 catgtcggtg atgtgcagac tctggcccgg gacttcacgc cgggagttgc ccctttcctc
     1321 ggatcgttga cccagccgct ccagccggcc acgacgccca tccaaggcaa gaagttttct
     1381 gatatctgga tgcgtgccgt gatcggcaac aacctgccga cactgatgac gccggcgtgg
     1441 ctggatgctc cggtaggcgg acgcgcggcg caggcgtggg ctctccggcc gccgatcaac
     1501 cggtatctga cggtcgatgc gttcttcgat ccctatcaac ctggggtgag tcaattcaat
     1561 accgaccttc gatttcaaga gggaaccaat tggtattttg aagtgg
//
LOCUS       DATMTI010000248         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_603863, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000248 DATMTI010000000
VERSION     DATMTI010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603863"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VN039_04200"
     CDS             complement(<1..995)
                     /locus_tag="VN039_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWV45219.1"
                     /translation="MNARYWIALILMLCPWPSWAMEHYSNVAQDQNGRAIVGATVTVY
                     TADTTTPASIYSDNGLTVKSNPFTTGIDGIYDFYAADGKYDIQITKFGYTTIYWDPNK
                     TKSLALFDSRQFIVPFGNTFPTTPPPDIGSWFVLTNDNAACTEGSGSNATPCRWDGTT
                     WVPMGGGGGGGGAAGLDANFDLTGGNVITGSSETKKWTLLGTGGQAANGGVKYQKSDG
                     TWIENCIFGGIEGNCDKYVNLNENKPWGIKNHLGNVVLYFDEVLGKYTTATIDCSQGS
                     VACSLVDERHFAVATCQNTTASANFDLPTSNAPTATCNTGSNTQKGYLAFNDTTE"
     gene            complement(1003..1596)
                     /locus_tag="VN039_04205"
     CDS             complement(1003..1596)
                     /locus_tag="VN039_04205"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HWV45220.1"
                     /translation="MMATKTQVNTDSVQTMENKTMTAPVVNGGTCGADPVLPLGIATK
                     QYVDNLLASILPPGTQLPYGGAAAPAGFLVCDGSAVSRTTYSLLFAAISTFYGVGDGS
                     STFNLPDKRGRTSIGAGTGAGLSPRIRGNKIGAEEATAPLPAHNHGNGATLFSQGAQG
                     GSGGQLVTVTATASAGSGTGIHNNMQPSEVDLWIIKT"
     gene            complement(1597..>2200)
                     /locus_tag="VN039_04210"
     CDS             complement(1597..>2200)
                     /locus_tag="VN039_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWV45221.1"
                     /translation="NAVRSIPSVVGGIPINIPTATEWSGGSYFASLVDIASVQVAGDV
                     RGVFVSGRRVKVYPYPIPGTVSLSEYDGRNTTISIVLDFGSLDNTVVSIAAATLSPAA
                     GPMPERRHIGTSTFLSGPITIPAASGMNLVPVGLIRLRTGSAAPGWLQCNGQAVSRTT
                     YALLFAAIGTAFGAGNGTTTFNVPTIADSGASHYWIYAQG"
BASE COUNT          457 a          623 c          625 g          495 t
ORIGIN      
        1 tgttccgttg tatcgttgaa tgcgagatag cccttttgcg tattactgcc ggtattgcac
       61 gttgctgtag gggcgttcga agtcggcaaa tcgaagttcg cgctcgccgt cgtattctgg
      121 caagtggcga cggcaaaatg ccgctcatcg actaatgagc aggcgacgct gccttgagag
      181 caatcgattg tcgcggtcgt atattttcct aatacctcat caaaatagag gacgacgttt
      241 cctaaatgat tttttattcc ccacggctta ttttcattca gattcacata tttgtcgcaa
      301 ttgccctcaa tgcctccaaa aatgcagttc tcgatccagg tgccatcgga cttctggtat
      361 tttacgccgc cattggcagc ttgcccgcct gttcccagca gcgtccattt cttggtttcc
      421 gatgatccgg tgatgacgtt cccgccggtc aaatcgaagt tggcatcaag cccagccgct
      481 ccgcctccgc caccgcctcc acccatgggc acccacgtcg tgccatccca tcggcacggt
      541 gtcgcatttg acccgctccc ttcggtacag gctgcattat cattggttag gacgaaccag
      601 gagccgatgt ccggaggtgg cgtcgtggga aaggtattcc cgaacggcac gataaactga
      661 cgcgaatcga acaaggcgag cgattttgtc ttattggggt cccagtaaat ggtggtatag
      721 ccgaacttcg taatttgaat atcgtacttt ccatcggcgg cgtaaaagtc atagatcccg
      781 tcaatccctg tcgtgaacgg attgctcttg acggtgaggc cgttatcgga atagatcgag
      841 gccggtgtcg tcgtgtctgc ggtatagacg gtgaccgtcg cgccgacaat cgcccgaccg
      901 ttctgatctt gcgcgacatt ggaatagtgc tccatggccc aggacggcca ggggcagagc
      961 atcagaataa gtgcaatcca atatcgtgcg ttcatacgag cctcacgttt tgatgatcca
     1021 gagatccact tccgacggct gcatattgtt gtgaatgccg gtgccgcttc cggcgctggc
     1081 cgtggccgtg accgtcacga gctgtccacc cgacccgcct tgagcgcctt gtgaaaagag
     1141 cgtcgcgcca ttgccgtgat tgtgtgccgg taacggcgcc gtcgcttctt cggccccgat
     1201 cttattgccg cgtatgcgcg ggctgagtcc tgcaccggtt ccggccccga ttgaggtgcg
     1261 ccctcgttta tcggggagat tgaatgtcga gctgccgtct cccacaccgt aaaacgtcga
     1321 aatggccgca aataagagcg agtaggtcgt ccgagagact gccgatccgt cacagacgag
     1381 aaatcctgcc ggggcggcgg cgccgccata gggcaattgc gttccaggtg gaagaatgct
     1441 cgccaagaga ttatccacgt attgtttcgt ggcaatcccg agcggcaaga ccggatcggc
     1501 cccgcacgtc ccaccattga ccacaggagc cgtcatggtt ttgttttcca tcgtctgcac
     1561 ggaatcggta ttgacctgtg ttttcgtcgc catcatttac ccctgggcat agatccaata
     1621 atgcgaggct ccggaatcag caatcgtcgg cacattaaac gtcgtcgtcc cgtttcccgc
     1681 cccaaaggcc gttccgatcg cggcaaagag cagggcataa gtggtgcggg agaccgcctg
     1741 cccgttgcat tgaagccacc ctggagccgc tgatccggtt ctgaggcgaa tgagtccgac
     1801 cggaacgaga ttcatgccgc tggcggcggg aatcgtgata ggtcctgaga gaaaggtcga
     1861 ggtcccaata tgccgacgct ccggcatcgg gcctgcggct ggggagagcg tcgcggcggc
     1921 aatcgacacg acggtattat cgagtgatcc gaaatccagc actatgctaa tagtggtatt
     1981 gcgcccgtca tactccgaca ggctgacagt tcctggaatg gggtagggat agactttgac
     2041 gcgccgaccg gacacaaaga cgccgcgcac atcgcccgcc acttgtacgg atgcaatatc
     2101 gaccaaggag gcgaaatagg acccgccgga ccattcggtc gcggtgggaa tattgatcgg
     2161 aatccctccg acgacggacg gaatggagcg gacggcattc
//
LOCUS       DATMTI010000249         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_688521, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000249 DATMTI010000000
VERSION     DATMTI010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688521"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..513)
                     /locus_tag="VN039_04215"
     CDS             complement(259..513)
                     /locus_tag="VN039_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HWV45222.1"
                     /translation="MDMTTALGLLAGMLTTIAFLPQVIRTWKSKSTKDLSLAMLGTFT
                     TGVFCWLIYGLMIDSLPIILANSITFVLAGANVILKLRYG"
     gene            complement(534..2825)
                     /locus_tag="VN039_04220"
     CDS             complement(534..2825)
                     /locus_tag="VN039_04220"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWV45223.1"
                     /translation="MKINRRSLLRFSLIWPATVPMWAGTLLQAAESKDDRRKETLTKE
                     RPAATDSLADAIVIKNENVFFVAQPDGNVPMRDSHGMGLYYRDCRYVNGYELALAGKQ
                     PAHLSASSAQGSIGVFTLTNPRIELVNGDILHEEKLGITWRRLMDNETPALRDCLLIQ
                     NFTLQPLEFPVSLTIQSAFEDIFAVRGLLPKQFGTLHRPSWEAGTLLWVYDGKDGVVR
                     TLTVHFDPLPDKHQGTTAEFAVRLSPRGQQRIDVSLVIGESKDRTTAHRMRPDPVDFS
                     RSESELERETQVWMAHVTRVATNGSALDQLMERSFRGLRVLRTHLGDETFFAAGVPWF
                     VTLFGRDSLITAWQMLAYHPRIAEQTLRLLAKYQGTKEDRWRDEQPGKILHELRVGEM
                     ANLDAVPHTPFYGTIDATPLFLMLIAEHAAWTGSLSLFEELRAPVERALSWMDTYGDR
                     HGEAYLTYASLSKDRLINQGWKDSGDAIVTADGEVAVPPLALVEVQGFGYAAKLGIAG
                     LFKRIGDQDRTDRLIREARRLRERFNRDFWMEDEGCYALALQADGRQAAVVSSNPGQA
                     LWTGIADQDKAQRTIRRLMAEDMFSGWGVRTLSSKERAYNPVGYHLGTVWPHDNSIIA
                     AGCRRYGQDRETLRIFHGLFDAAYHFHAHQLPELFCGFGRAQYEIPINYPVACHPQAW
                     ASGSLPFFLTTLLGLIPEAFEKRLRIRRPLLPDFLDRLELRRLHVGGAVVDLAFRRGT
                     QGVQVDVVKQQGELDVQMDNEEH"
     gene            3150..3341
                     /locus_tag="VN039_04225"
     CDS             3150..3341
                     /locus_tag="VN039_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45224.1"
                     /translation="MRTRDWIIPFGMVLLSVGLAFSMEALDEGFRGHLAPVWWVFSGG
                     QQIARQFLSAMASHSIKLR"
     gene            complement(3451..>3905)
                     /locus_tag="VN039_04230"
     CDS             complement(3451..>3905)
                     /locus_tag="VN039_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWV45225.1"
                     /translation="MSNAEIIGVLNAIDHNEIDAGQLALQKAPTPEVRSFASRMVREH
                     EMMMQNTDRLAHRLSVQPQNPALVSTMADTHREMMKKLGAKYGSDFDKAYIGYQIKMH
                     EQALDLVKDVAESVENRSLKLHLREMRPDLQNHLTSAQSIEQQVVAQQ"
BASE COUNT          827 a         1142 c         1128 g          808 t
ORIGIN      
        1 caagggcgag gtcaaggcct tgaaggaaga agcgaaggga aatgatatga aagccgccgg
       61 agagcgggca aaaggcaatg tcaaaggcgc cgttgagaag gggaaaggaa aaacaaagga
      121 gctgaaggag aagatgaagg attagatcga cctgaccaca gcaaaatgcc gcgctgttcg
      181 ggcgagatca gacgcgttaa agaggagtcc gccaccgttc cgtcgtcgga caagcgcggt
      241 cctgacctcg cacatacttt agccataacg taacttcagt atgacgttcg cgccggccag
      301 cacgaatgtg atggagttgg cgaggatgat gggtagcgag tcgatcatca gtccatagat
      361 cagccagcag aagactccgg ttgtaaacgt gcccagcatg gccaacgaga gatctttggt
      421 cgatttgctc ttccaggtcc ggatcacttg cggcaaaaag gcgatcgtgg tcagcattcc
      481 ggccagtaat cccaatgcgg tggtcatgtc catggtctcg acctcagttc tgtttagtgc
      541 tcctcattgt ccatctggac gtccagttcg ccctgctgct tcaccacatc gacctgaact
      601 ccctgcgtcc cgcgtcgaaa cgcgagatcg acgaccgctc cacccacatg cagccgccgc
      661 agttccaatc ggtcgagaaa atcgggcaac agcggccgcc tgatacggag ccgcttctcg
      721 aacgcctccg gaatcaagcc gagcagggtc gtcaagaaaa acggaagcga gccggaagcc
      781 caagcctgcg gatggcaggc aacaggatag ttgatcggga tctcatactg cgcgcggccg
      841 aagccgcaaa agagttccgg aagctgatgc gcatggaaat gataggcggc atcgaacagt
      901 ccatgaaaga tccgcaacgt ctcccgatct tgtccgtaac gccggcatcc cgcggcgatg
      961 attgaattgt catgcggcca aaccgtgccg aggtgatagc cgacgggatt ataggcgcgt
     1021 tccttcgacg acagggtgcg gacgccccat ccgctgaaca tgtcctcagc catgagtcgc
     1081 ctgatcgtcc gctgggcttt gtcctggtcc gcgatgccgg tccacagcgc ctggcccgga
     1141 ttggaggaga cgacggcggc ttgccgcccg tccgcctgca atgcgagcgc gtaacagcct
     1201 tcatcctcca tccaaaaatc ccggttgaac cgctcacgca gccgccgcgc ctcgcgaatc
     1261 aagcgatccg tccggtcttg gtcgccgatc cgcttgaaca acccggcaat cccgagtttg
     1321 gcggcatagc cgaagccctg cacctcaacc agcgcgagcg gcggcacggc gacttcgccg
     1381 tcggcggtca caatggcatc gcccgaatct ttccacccct gattgatcag tctgtctttc
     1441 gatagactgg cataggtgag ataggcttct ccgtgccgat cgccgtaggt atccatccag
     1501 gacaacgccc gttcgacggg agcgcgcaat tcctcgaaga ggctcagaga accggtccac
     1561 gccgcatgtt ccgcgatcag catcaagaac agcggggtgg catcgattgt cccatagaac
     1621 ggagtgtggg gaaccgcgtc caggttcgcc atctcgccga cccgtagttc atggagaatc
     1681 ttgcccggct gttcgtcacg ccatcggtcc tctttcgtgc cttgatactt tgccaagagg
     1741 cgcaaggtct gttcggcgat ccgcggatgg taagccaaca tttgccaggc ggtgatcagg
     1801 ctgtcgcgcc cgaatagtgt aacgaaccac ggcactcccg cagcaaaaaa ggtctcgtca
     1861 cccagatgtg tgcgcaaaac ccggagtcca cggaaggagc gctccatgag ttggtcgagg
     1921 gccgagccgt tggtcgccac cctggtgaca tgcgccatcc aaacctgcgt ttctcgttcg
     1981 agctcgctct ccgatcgact gaagtccacc ggatcgggcc tcatacgatg ggccgtcgtc
     2041 cgatccttgg attcgccgat cacgagcgag acatcgattc gctgttgacc gcgcgggctc
     2101 aagcgcacgg cgaactccgc cgtcgtgccc tgatgcttgt cggggagcgg atcgaagtgg
     2161 accgtgaggg tgcggacgac gccgtcttta ccatcgtaga cccacagcaa cgtgccggct
     2221 tcccaggacg gccgatgaag ggtgccgaac tgcttcggca gcagccctcg gacggcaaag
     2281 atatcctcga aggcggattg gatcgtcagg ctgacgggaa actcgagcgg ctgaagcgta
     2341 aagttctgga tgagcagaca atcgcgcagc gccggagttt cgttgtccat gagccgccgc
     2401 catgtaattc caagtttctc ttcatggagg atatcgccgt tcaccagctc gattctgggg
     2461 ttcgtcaatg taaagacgcc gatcgaacct tgggccgacg aggccgagag atgagccggc
     2521 tgcttgccgg ccagggccaa ctcatagccg ttcacgtagc gacaatcgcg atagtaaagg
     2581 cccatgccat gagagtctct catgggtacg tttccgtccg gctgtgccac gaaaaagaca
     2641 ttctcgttct tgatgacgat cgcatcggct aggctgtccg tcgcggccgg acgttccttc
     2701 gtgagcgtct cctttcgccg atcatccttc gattcggccg cctgcaacaa tgtaccggcc
     2761 cacatcggca cagtcgccgg ccagatgaga gagaaacgaa gcagcgagcg ccgattaatc
     2821 ttcatggcag ccgattcgct tgggaccaac caaagccaat cttcgtcccc tctgcgtggc
     2881 ggaagaggat cgcaggccga acggttcaaa tcggacgcag tccgtcatgc ggctattcgc
     2941 attttcggaa caacaatagc aaaccctata gcgccccctg gctagaagaa acccttgtcc
     3001 gtctcatccc tcgagatggg acgacattcc ctctgcaaca aggcgaagga gatagggcaa
     3061 gggcatatcg tttgcctttt tgtgtcggac aagatgctcg gcggtgaatc tatcgccaat
     3121 tccgacacgt cgttcgctcg ctcgatcggg tgcgcacgcg cgattggatc attcctttcg
     3181 gtatggtctt gctctccgtg ggtctcgctt tttcgatgga agccctcgac gagggatttc
     3241 gaggtcatct cgctccggtg tggtgggtat tcagcggcgg acagcagatc gcgcggcagt
     3301 tcctctccgc catggcatcc cactctatca agctgcgttg acgatcagct caccggctta
     3361 aagcgctttt tgtcacggtc tcttcgctag ccgatcgccg caggggggtg aggagacaac
     3421 tgcttccgtc cgtctcctct tcacggattc ttactgttgc gcgacgacct gctgctcgat
     3481 ggattgtgcg gaggtcaagt ggttctgcag gtccggcctc atctcgcgca gatgcagctt
     3541 aaggctccgg ttttcaacag attcagccac atccttcaca aggtcgagcg cctgttcatg
     3601 cattttgatc tgatatccga tgtacgcctt atcaaagtca gagccatatt tggcaccgag
     3661 ttttttcatc atctcccggt gagtatccgc catcgtggag acgagagccg gattttgggg
     3721 ctgcacgctc aaacgatggg ccaaccggtc ggtgttctgc atcatcattt catgttcgcg
     3781 caccatgcga gaggcaaatg agcgtacttc cggggtgggt gccttctgca gggcaagttg
     3841 tccggcatcg atttcattgt gatcaatggc attgagtacg ccgataattt cagcattcga
     3901 catgg
//
LOCUS       DATMTI010000250         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_137192, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000250 DATMTI010000000
VERSION     DATMTI010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137192"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VN039_04235"
     CDS             <1..727
                     /locus_tag="VN039_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWV45226.1"
                     /translation="PLSICLLGTFLVTSAQAQEAVKGVVELFTSQGCASCPPADEALR
                     KMIQKGDVVGLSYHVDYWNYLGWTDSLASKENTDRQYGYMRALGRNGVYTPQAILNGR
                     DHVKGADVRGIYDRLDAFKREGKGLNVPVSSKFAGDEVEIDIGAGSGKADVVVAYFTR
                     EQTVDVQKGENQGKKMSYWHSVYDVQSVGMWDGSPMTVKLPASVVAKVKKGGCAVLLQ
                     TANAGGDPAAIVGASILLGNETP"
     gene            1128..1520
                     /locus_tag="VN039_04240"
     CDS             1128..1520
                     /locus_tag="VN039_04240"
                     /inference="COORDINATES: protein motif:HMM:NF022437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HWV45227.1"
                     /translation="MTDQPDVQQVQTASHDNSTVIDLREYKKNKDPLPVTFHRRELDA
                     ILRIYGRMVGEGEWRDYAIDHLKEKAVFSVFKRSGEMPLFRIEKNPKLAAKQGAYCVV
                     NTDGRILKRGHELPQVLKVFDKVLKLIE"
BASE COUNT          362 a          413 c          508 g          365 t
ORIGIN      
        1 ccccctgtcg atatgtcttc tcggcacgtt tctcgtcacc tccgcgcagg cgcaggaggc
       61 cgtgaagggc gtcgtcgaat tgttcacatc gcagggatgt gcctcatgcc cgccggcgga
      121 tgaagctttg cgcaagatga tccagaaggg cgatgtcgtc ggtctttcct accatgtcga
      181 ttactggaac tatctgggct ggacggattc gctggcatcg aaggaaaata ctgatcggca
      241 atatggctat atgcgcgcac tcggtcgcaa cggggtctat acgccgcagg ccattctgaa
      301 cggccgcgac cacgtcaagg gagcggatgt gcgcggtatc tatgacaggc ttgatgcatt
      361 caagcgcgag ggcaaaggcc tgaatgtgcc ggtctcgtcg aaatttgccg gtgacgaggt
      421 ggaaatcgat attggcgccg gcagcggcaa ggccgacgtc gtcgtggcct atttcacccg
      481 cgagcagacc gtcgatgtgc agaagggcga aaaccagggg aaaaaaatgt cctactggca
      541 cagcgtctat gatgtgcagt ccgtcggcat gtgggatggg tcaccgatga cggtgaagct
      601 tccggcgagt gtggtggcaa aggtgaaaaa gggcggctgc gctgtgcttt tgcagacggc
      661 taatgccggc ggtgatcctg cggcaatcgt gggtgcaagt attctgcttg gaaatgaaac
      721 cccctgatcg cctgtcgggc gatggaagat ccggtccggg cattgggggc tgggggtaaa
      781 gggtcaatgc accggaccgg gtcatcttgg tgcctaggca tcaattgaca ataggactat
      841 tccatccaat tcgggccatg gtttgaccga aatacggcag gccatgtgaa aatggctgcc
      901 taaatggtta ccatttttaa cctctgcttt tgccgtcggc agtcactcca gagggatgga
      961 tcgggcgcca ttccgtgctg gagccttctg gatcggctgc agttcgaggc ctgtgaaacc
     1021 tttcccggaa ttgccggtcc tgtgcgtcac gatagtgcaa agacttgcaa tttcgccccc
     1081 tcaggctcac actacatgga tgatttgggt ggaatcggag gtattggatg accgatcaac
     1141 cggatgtgca gcaggtgcag accgcttcac atgacaattc caccgttatc gatctccggg
     1201 aatataaaaa gaacaaggac cctttgcctg tcacctttca ccggcgcgag ctggatgcga
     1261 tcctgcgcat ctatggccgc atggtgggag agggcgagtg gcgtgactac gccatcgatc
     1321 acctgaagga aaaggcggtg ttttccgtct tcaagcggtc aggtgaaatg ccgcttttca
     1381 ggatcgagaa aaaccccaag ctggcggcca agcagggggc ctattgcgtg gtgaatacgg
     1441 atggccggat tctgaaacgt ggccatgaat tgccgcaggt tctgaaggtt ttcgacaagg
     1501 ttctgaaact catcgaatga cctttcgcag aagcggactg catgcctctg gcggataagg
     1561 gtgacaaatc tttgcaaata aaaaggcggc cgaagggccg cctttcgcat gtctggcaat
     1621 cgaatcccta accttagtcg ttgcgatt
//
LOCUS       DATMTI010000251         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_392090, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000251 DATMTI010000000
VERSION     DATMTI010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392090"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..681
                     /locus_tag="VN039_04245"
     CDS             73..681
                     /locus_tag="VN039_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWV45228.1"
                     /translation="MDFLTNIEEIRKRARRHIEEGSVTERYALDRKQAIRVLNDALAT
                     ECMCVLRYRFHYFMATGINSAAVAEEFMEHAQEEQVHADQLAERIKQLGGKPELHPAV
                     IAERSHSEYREGTSLADMIREDLIAERIAIETYREMVRYFGDKDTTSRVMMEGILAKE
                     EEHADELADLLFAVQPDTNQGSRRLYFSDEVPRHAEGEKVKS"
     gene            723..1244
                     /locus_tag="VN039_04250"
     CDS             723..1244
                     /locus_tag="VN039_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWV45229.1"
                     /translation="MAKRQMKSNQDDDEPNEAPAESVHALDLLKEDHRKVEQLFQSFE
                     TADGRSRQGIADEAIKALEIHTKVEDGLVYPAIREATGEEDLVDEATEEHHVVTLVIK
                     ELKKMKASAEGYKAKFTVMSELVRHHIEEEEKEMFPQAENADIDWEELGREALSIKER
                     MMSKSSRGQRRAA"
     gene            1597..>1827
                     /locus_tag="VN039_04255"
     CDS             1597..>1827
                     /locus_tag="VN039_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HWV45230.1"
                     /translation="MTENPSEKERYHTNTQDFGWNEERAEFEDHRENIGDVQRVLSVI
                     AGMVFLASGLFRRNRRRNWSGAALVTAGGGLLY"
BASE COUNT          498 a          422 c          555 g          352 t
ORIGIN      
        1 caaagtttat gcagcgcagg gttgagtctc ggaagaaatg acgaggtatc aataggaaga
       61 ggggaggaag ctatggattt cctgaccaac atcgaagaga ttcgaaaacg agcccgtcgg
      121 catattgaag aaggttctgt caccgagcgg tatgcgctgg accggaaaca ggctatccga
      181 gtgctgaacg atgcgttggc taccgagtgt atgtgcgtgc ttcgttatcg attccactat
      241 ttcatggcga caggcatcaa ctcggccgca gtggccgaag aatttatgga gcatgctcag
      301 gaggaacagg tgcatgccga ccaactcgcc gagcgaatta agcagttggg aggcaagcct
      361 gaacttcatc ccgctgtcat tgcagagcgt agccacagtg aatatcggga aggcacgtct
      421 ttagccgaca tgatacgcga agatctgatc gccgagcgta ttgccattga gacatatagg
      481 gaaatggtgc ggtacttcgg cgacaaggat acgacttccc gcgtcatgat ggaaggcatc
      541 ttggcgaagg aagaagagca tgccgacgag ctggcggact tgctgtttgc cgtgcaaccg
      601 gacacaaacc agggctcgcg gcgactgtac ttcagcgatg aggtgccgag acatgcagaa
      661 ggagaaaagg tgaaatcctg atggagcaac ctgatcgatg cacatgcaag gagggagtcg
      721 cgatggcaaa aagacaaatg aagtcgaatc aagacgatga cgaaccgaat gaggccccgg
      781 cggagtctgt acacgcactg gatcttttga aagaagatca tcggaaagtg gaacagttgt
      841 ttcagtcgtt tgaaacggcg gacggtcgtt cccggcaagg gattgccgat gaggcgatca
      901 aagctctgga aatccataca aaagtcgagg acggcctcgt gtatccggcc attcgtgaag
      961 cgaccggtga agaggacttg gtcgatgaag ccactgaaga acatcacgtg gtcacgttgg
     1021 tgatcaaaga gctcaaaaaa atgaaggcat cggccgaggg ttacaaggcg aagtttacag
     1081 taatgagcga gctcgtgagg caccacattg aagaggaaga gaaggaaatg tttcctcaag
     1141 cagagaatgc ggatatcgat tgggaggaat taggacggga agctctcagt atcaaggaac
     1201 gaatgatgtc caaatcctcc cgaggccaac gtcgagccgc ataagaatga gaacagcgag
     1261 ccaaggcgcg gcatagaaac agcgtcacca cgtccctgac gaagcgcggg ggcaggtcac
     1321 gccacggcaa gtccgtgcgg gcgatcccca acaccgcttc gacaaaggag cgattgtcga
     1381 gcgctggtca ccccgcgttc gctggccttg cccgagcaca acggctcgat tcgttttcat
     1441 tggtcgtcgc gcaatatctt ccggtccatc ctcgcaagct cccgaaagcg agatgtatct
     1501 acgtttgccc tttgagaaca gaccctagta gcttacccat cacatgacat gcacatgacc
     1561 tgaggagaat tcatgaggag aactgaggag gagccgatga ctgagaatcc gtcggagaaa
     1621 gagcgatatc atacaaatac gcaggatttt ggatggaatg aagaacgagc ggagttcgag
     1681 gatcatcgag aaaacatcgg cgatgtgcag cgggtgctat ccgtcatagc aggaatggtc
     1741 tttctagcct ccgggttgtt tcgccgtaat cggcgccgta actggtcggg tgcggcgctg
     1801 gttacggcag gagggggact tctctat
//
LOCUS       DATMTI010000252         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_561492, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000252 DATMTI010000000
VERSION     DATMTI010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561492"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2055
                     /locus_tag="VN039_04260"
     CDS             <1..>2055
                     /locus_tag="VN039_04260"
                     /inference="COORDINATES: protein motif:HMM:NF022063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HWV45231.1"
                     /translation="RYAIWIGEAKALDGDDEHTPWLTPERRQGWRYWSDYEDFLEPKI
                     AEASVESLDRQTDEILKRLEDPEKPGPWDRRGLVVGHVQSGKTSNYTGLICKAADAGY
                     KIIIVLAGLHNNLRSQTQMRLDEGFLGYETSPKLNSPKKEIGVGLGREPSLRPNYVTN
                     RSDGGDFRTQVAKHLGIRPEQTPWLFVVKKNKTVLTRLLEWIRLHVAEQTDPETGRRV
                     VTKLPLLLIDDEADHASVDTAEQAFDGDGNPDPEHEPKVINSLIRQILGAFSRSAYVG
                     YTATPFANIFIHEKGWTKLEGEDLFPRSFIVNLPAPSNYVGPMKVFGHTLSDGTPVEG
                     LPLIRDVSDFTDSEEPGSAVGWMPNGHKNGYSPLYKGQPELPPSLREAIQAFVLTCAI
                     RRTRGQGKEHCSMLIHVTRFTSVQAEVTGQVKSYVEDMYRHLRRGIGREEVVAELRAL
                     LEQDFIPTTRLVNQRMPPNERVDEPDWDTVEPELMSVLEELQNRTRAVNGTAKDVLDY
                     ADNEVTGIRVIAVGGDKLARGLTLEGLTVSYFLRASRMYDTLMQMGRWFGYRPGYTDL
                     SRLYTTEELQKWFRHITIASEELRQAFDHMSATHAKPREYGLRVQSHEVMTVTSSTKM
                     RSGRELKMSYAGDGIETTVLSRDKAVIESNADAVERLLAGMGKPILNHSQPRPGNTSA
                     SWS"
BASE COUNT          490 a          587 c          606 g          372 t
ORIGIN      
        1 cgctacgcga tatggatcgg cgaggcaaag gcgctcgatg gtgatgacga acatacgccg
       61 tggctgaccc ccgaacgcag gcaggggtgg cgctattggt cggactacga agactttctc
      121 gagccaaaaa ttgcggaggc aagcgtcgaa agccttgacc ggcagacgga tgagatcctc
      181 aagaggctcg aggatcccga gaagccgggc ccttgggaca ggcgtggctt ggtcgtcggc
      241 catgtgcagt ccggcaaaac gagcaactac acaggtctta tctgcaaggc cgccgatgct
      301 ggctacaaga tcatcatcgt gctcgccgga ctccacaaca atctcagatc gcagacacag
      361 atgcggcttg acgagggttt cctggggtac gagacgagcc caaaactcaa ctcaccaaaa
      421 aaggaaatcg gggtcggtct cggccgggaa cccagcctga gacccaacta cgtcaccaat
      481 cgatccgacg gaggtgactt ccggacgcag gtggccaagc acttgggtat caggccggaa
      541 cagacgccat ggttgttcgt ggtcaagaaa aacaagaccg tccttacgag gttgctcgaa
      601 tggatacgtc ttcacgttgc agagcagacc gacccggaaa cgggacggcg cgtcgtgacc
      661 aagctgcccc tgctcctcat cgatgatgaa gctgaccatg catcggtgga caccgcggaa
      721 caggcatttg atggtgatgg caatccggac ccggagcatg agcccaaagt catcaacagt
      781 ctgatacgac agatcctcgg ggcgttttcc cgttccgcct atgtcggata cacagctaca
      841 cccttcgcaa acatcttcat ccatgagaag ggatggacga agcttgaagg cgaggatctg
      901 ttcccccgca gcttcatagt caacctgccc gcaccatcga actatgtcgg gccaatgaag
      961 gtgttcgggc ataccctgtc ggacggcacg ccggtcgagg gacttccctt gatcagggat
     1021 gtatcggact tcaccgacag cgaggaacca ggctcggccg ttggctggat gccgaacggg
     1081 cacaagaacg gatattcacc gctctacaag ggacagcccg agctgccacc aagcctgagg
     1141 gaggccattc aggcttttgt cttgacctgc gccatcaggc gaacgcgggg ccaaggcaaa
     1201 gagcactgct cgatgctgat ccacgtcacg agattcacga gtgttcaggc agaggtgacg
     1261 ggccaggtga agtcctatgt cgaggacatg tatcggcacc tgcgccgcgg tataggtcgg
     1321 gaggaagtcg tcgcggaact ccgcgcactt ctggaacagg atttcattcc aacgacacgg
     1381 ctggtcaacc aacgcatgcc gccgaatgag cgtgtcgatg aaccggactg ggacacggtg
     1441 gaaccggaat tgatgtcggt tctcgaggag cttcaaaacc gtacacgcgc ggtgaacgga
     1501 accgcgaaag acgtcctcga ctatgccgac aacgaggtga ccggaattcg ggttatcgcc
     1561 gtaggtggcg acaaactggc ccgcggactc acactggaag ggctgaccgt cagctatttc
     1621 ctgcgtgcct cgcgaatgta cgacaccctc atgcagatgg ggcgctggtt cggataccgc
     1681 cctggatata cggatctatc gcggctctac acgaccgaag agctccagaa gtggttccgc
     1741 cacatcacca ttgcatcaga ggaacttcgc caagcctttg atcacatgtc agccacacat
     1801 gccaaacccc gcgaatacgg ccttcgtgtg cagtcgcatg aagtcatgac ggtcacttct
     1861 tccacgaaga tgcgatcagg tcgtgagctg aagatgtcct acgccgggga tggtatcgaa
     1921 actaccgtcc tttccaggga caaggcggtg atcgagtcga atgccgatgc tgtagagcgc
     1981 ctgcttgcag gcatgggcaa accaattctc aatcactcgc aaccgcggcc gggcaataca
     2041 tcggcctcat ggagc
//
LOCUS       DATMTI010000253         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_477119, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000253 DATMTI010000000
VERSION     DATMTI010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477119"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..2148)
                     /locus_tag="VN039_04265"
     CDS             complement(133..2148)
                     /locus_tag="VN039_04265"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWV45232.1"
                     /translation="MIEAAGALFRCLQLASSMLLIGGCVFLAIAELKASASGHTWPTR
                     LKQTFPWLAVTVLLGLFGLLATTTAQATGVPENVRNPQAWLDFLQKTRIGLIWTLRTA
                     SALIVLVIVLYVRISPSARWRYVICASAAALPLVLGALASHSAAEEQAILATVAYALH
                     LITASVWFGGLPGVILVAFTTTTGSTDDRSQAGEVLNRFSALALPTMIAIVVSGLVVA
                     NRMIDTNYAGLVATTYGWLLMAKLTLLAIILAIASRAKSVWVPSLKQSPDTAAVGGRK
                     LRTWVRTEFVLAIILVIVATLLASAVPAKHDVIDNWPYPFRFSIDANWGDWLVRVIVL
                     TGLVLLIIAGTTIVLGRKKQWDTSWRITVPTILGIVGLGATLYPLSVQSYPETYRKTP
                     VPFDSISIANGAELFAANCIPCHGPQAKGNGVLAKTLPKQPVDLLTEPHTAMHTAGDF
                     FHWLTYGRFNGIMPAFGGKFSEEERWDLLNFLHANSRGYQSRIITPRILPEQPFMATP
                     NFSYTAHDGSSGTLKDFRGRQDVLLILFSWPESRVRLNQLRAAAISITGKNTAILAVP
                     MTDLTPDELAAAVRDMPFPVVTQGAHEISSSYALFRRTLSIPDLFGEGTSPKHMEFLI
                     DRFGYLRARWIPNGDGSEWADITLLTQQITQLNQEREILPPPGDHVH"
     gene            complement(2145..2546)
                     /locus_tag="VN039_04270"
     CDS             complement(2145..2546)
                     /locus_tag="VN039_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HWV45233.1"
                     /translation="MIGSPGKHTFRPVILIVAFVLGIPVTSAFAHSMLVKAEPPRRAI
                     LTKAPIQVRLWFNEKIEGDYASLVVLDAKKQPITDLRPTLASDDPKSIVLPLPELAPG
                     KYSVKFRVLSVDGHVVESSFDFTVKGEAQKK"
     gene            complement(2636..3223)
                     /locus_tag="VN039_04275"
     CDS             complement(2636..3223)
                     /locus_tag="VN039_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45234.1"
                     /translation="MSCHMLIRAWVCCLFVLVLAPFSALAQHGHELATNTCVLHIGPY
                     KMYFNSYQPDTQFDRQFCQELPGTGNTVLVLDYVEQELRSLPVEVRIIRDTGSEENLE
                     AITVAHLPAKVYATGSIDVKYNFDKPGKYIGLVSIGDKHEHLSRFPISIGETGVMSHF
                     KHYIMVIVPVLVGIAVAVFFSFRDRRKPSGIPVSS"
BASE COUNT          804 a          885 c          974 g          713 t
ORIGIN      
        1 atacgcctgc gggtgttgga agtcaagatc agcagagatg cctcgtggcg tcagtctgct
       61 tgcaggacga cgtgaaaggc ctgtctggtg caagaaagag taaccaggtc aactacgtaa
      121 aagggaggct cttcaatgaa cgtgatcacc cgggggaggc aagatttctc gctcttgatt
      181 gagctgggtg atttgttgtg tcagcaaggt tatatccgcc cactccgatc cgtctccatt
      241 gggaatccac cgtgcccgca aataaccgaa gcggtcgatg agaaattcca tgtgttttgg
      301 gctcgtccct tcaccgaaca gatccggaat agagagtgtc cttcggaaca gagcatagct
      361 gctggagatc tcgtgtgcac cctgtgtgac gacagggaaa ggcatatccc gtacggccgc
      421 ggccaattca tccggagtca agtcagtcat agggactgcc aagatggccg tattttttcc
      481 agtgatcgag atggcagccg cacggagttg attgagccga acacgcgatt ccggccagga
      541 gaaaaggatc agcagcacgt cttgcctccc acggaagtct ttcaatgtgc cgcttgagcc
      601 gtcgtgcgcg gtatacgaga aattcggagt cgccatgaac ggttgttcag gcagaatacg
      661 gggggtgatg atcctcgatt gataccctcg ggaattggca tggagaaagt tcagcaggtc
      721 ccagcgttct tcttcggaaa atttcccgcc gaaggccggc atgatgccgt tgaatcgtcc
      781 ataggtcagc caatggaaga agtcaccggc cgtgtgcatc gcggtatgcg gttccgtcag
      841 aaggtctacc ggttgtttcg gcaaggtttt ggccagtacg ccatttcctt ttgcttgcgg
      901 cccatgacac ggaatgcaat tcgcggcaaa taattcggcg ccgttggcaa tggagatgga
      961 gtcgaacggc accggagttt tccgataggt ttcgggatat gattgcacag aaaggggata
     1021 gagtgtcgcg ccaagtccga cgattcctaa gattgtcgga acagtgattc gccacgaggt
     1081 gtcccactgc ttcttgcgac cgagaacgat cgtcgttccg gctataatca ggaggacaag
     1141 gcctgtcagc acgataactc ggacgagcca gtcaccccag ttggcgtcaa tcgagaaacg
     1201 gaacggatag ggccaattat cgatgacgtc gtgcttggcg ggtacggcgc tggccaacaa
     1261 tgtggcaacg atcaccagta tgatggcgag aacgaactcg gtcctcaccc aagtccggag
     1321 ctttcgtccg cctactgcgg ctgtgtccgg actctgcttg agcgacggca cccacacaga
     1381 tttcgctcgc gaggcgatgg ccagaatgat ggcaagcaac gtcaactttg ccatgaggag
     1441 ccatccgtat gtggtcgcga ccagtccggc gtaatttgtg tcaatcatgc gattggccac
     1501 gacgaggccc gacacgacga tggcaatcat cgtggggaga gccaatgccg aaaaacggtt
     1561 caacacttcc ccggcctggg atcggtcgtc agttgatccg gtcgtggtgg tgaaagcaac
     1621 aaggatcacg cccgggaggc cgccgaacca gacactggct gttatgagat gcagtgcata
     1681 ggccactgta gccaagattg cttgctcttc cgccgccgaa tggctggcga gagcgccgag
     1741 gaccaagggc agcgccgcag ccgacgcgca gatgacgtag cgccatcgcg ctgaagggga
     1801 aatcctgacg tataggacga tcacaaggac aatgagggca ctggcagtgc gcagggtcca
     1861 aataagtccg atgcgggtct tttgtaagaa gtctaaccag gcttgcggat tccgaacatt
     1921 ttcaggaact ccagttgctt gcgccgtcgt tgtggcgagg agaccgaata atccaagcaa
     1981 cactgttacg gccagccatg gaaaggtctg tttcagacga gtcggccacg tatgtccgga
     2041 agcagaagcc ttcagctcgg cgattgccaa aaaaacgcaa ccaccgatca gcagcataga
     2101 ggaggccagc tgcagacagc gaaacagcgc accagcagcc tcgatcattt cttttgtgct
     2161 tcgcccttta cggtgaagtc gaaagacgac tcaacgacat gtccgtccac cgagaggacg
     2221 cgaaacttga cggaatactt gccgggcgcc agctccggca atggcagaac gatcgatttc
     2281 ggatcgtcag aggccaaggt gggtctcaaa tcggtgatcg gttgtttctt ggcatcgagg
     2341 actacgagag aggcataatc tccctcgatt ttctcgttga accacaagcg cacttgaatc
     2401 ggcgccttgg tgagaatggc ccgtcgtggt ggttccgcct tgaccaacat tgaatgtgcg
     2461 aaggccgagg tgacggggat tcccaagacg aacgccacga tcaagatgac aggcctgaac
     2521 gtgtgcttgc ctggtgagcc gatcattggt cctccatgtg ctcgattatg gtatccaagg
     2581 aagtgtgtcg catccggctg tctcactcag tgctccagcg ggcatccgtc gcctcttatg
     2641 atgacacagg gattcccgaa ggtttgcgac gatcccgaaa ggaaaagaac acggctacag
     2701 cgatgcctac caggacgggc acaatgacca tgatgtagtg tttgaagtgg gacatcacac
     2761 ctgtttcacc gatcgaaata gggaaccgcg agagatgttc atgcttatca ccgatcgaaa
     2821 ccaaaccgat gtacttgccg ggcttatcaa agttatattt tacgtcgatg gatcccgtgg
     2881 cgtagacctt ggcgggcaaa tgggcaaccg tgatcgcctc gagattctcc tctgagccgg
     2941 tatccctgat gattcttacc tccacgggaa gagaacgaag ttcctgctcc acataatcca
     3001 gcaccaaaac cgtgttacct gtccctggga gttcctggca aaactgtctg tcgaactggg
     3061 tgtcgggctg gtaactgttg aagtacatct tgtacgggcc gatgtggagg acacaggtat
     3121 tggtcgctaa ttcatgtcca tgttgggcca gcgccgagaa cggggctagg accagcacga
     3181 acaagcaaca cacccaagct cgaatcagca tgtgacagga catgttcaac cctctcttct
     3241 ttctctgctt ctcatgatcc gtaacggtcc cgtcgtcgcg ctatcctagg aagcagcgat
     3301 ttgcgacggc ttgccgcggt cgcgcatgaa aaagacaatc gcaccgacaa tgactaccgc
     3361 ggctactgga accata
//
LOCUS       DATMTI010000254         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_561531, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000254 DATMTI010000000
VERSION     DATMTI010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561531"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="VN039_04280"
     CDS             <1..1689
                     /locus_tag="VN039_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV45235.1"
                     /translation="TNEELYLFQKFLRMAVGTNNIDSSARYGHVNGLHAMQLVQGTHR
                     WTVTFDDILDADVLILVGTNITETNPITGLKVKEAVKKRRATLMTIESLEPVVDTMSN
                     IANLSHHHFRIPTDDTRHAILGLVKAVVEQNLTHPDLAQRYPAYLNAITSALQQTSWQ
                     DLQAATGIEPDSFMRAAKVAAGARRVVILAGQLLLRSEHGYSGCVTLLDLLLLIGKLD
                     GPGCGFAPLAEENNDQGAVEMGTVTEFLPGARPVASDTDRERIAKQWKGELPSDRGAS
                     LIEMLDRADAGSLKAMFIVGENPVGSLPATIHAEQSLRNLDLLVCQELFLTETAALAH
                     VVLPAASSLEKHGTFTNTEGHVQAVRPSIEPIGESRPDWEVFSALSILLNSPMEYAES
                     KEILKEIRSFLPGYGSLGPAPLPPKVDPSAVDRYLTTGYQRDLATRYQPIPRTSKPDG
                     TVRLDLTQSLFHSGKLSTRSKGLLQLEGSGRLRISPLDAARFALSDGDRVRLSSTSGE
                     MTTEVKIMERVPQGTAWFPYHFGQAAVRLFECAVDPITRVPSFRTATVSVMKVA"
     gene            1736..2812
                     /gene="nuoH"
                     /locus_tag="VN039_04285"
     CDS             1736..2812
                     /gene="nuoH"
                     /locus_tag="VN039_04285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWV45236.1"
                     /translation="MTEFGLRLAISLTQIAGVMGIVVVTVLILTLAERKVLGWMQDRM
                     GPMEVGPYGILQPFADAIKLFFKEDIIPAGANKFLFTVAPILCLIPAFIGFAVIPWGP
                     NQTFEIDGITVRPFVISDINIGILYILAFASLGAYGIILGGWASNSKYSLLGGLRSAA
                     QIISYELNVGLSIVGVLILAGSLSLVKITDAQAGGFWNWYLFALPAPQIFAFVVYVIS
                     SVAETNRVPFDLPEAESELVAGFFTEYSGLRFAFFFLAEYANMVLVSCVAAALFLGGW
                     NAPYPGTIMALIGLPSLAWVENTMWFAVKTYSFLFLFFWLRATLPRLRYDQLMRFGWK
                     VMLPIALGNIVVTSLAVFFYQQMK"
     gene            2827..3393
                     /gene="nuoI"
                     /locus_tag="VN039_04290"
     CDS             2827..3393
                     /gene="nuoI"
                     /locus_tag="VN039_04290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWV45237.1"
                     /translation="MISTATTKRFNLYEWFKTITFYEILVGMKATLSHLLHYRPVTVQ
                     YPHEKRTLPDNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPGEPTKRY
                     SKEYYMDMTRCLFCGMCVDACPVDALGMTREFEWAVYDKRQLHLDKQQLLAIGDRSFP
                     VREKRLELQHPNVAFFNVAFKHVPPKPN"
     gene            3483..>3761
                     /locus_tag="VN039_04295"
     CDS             3483..>3761
                     /locus_tag="VN039_04295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWV45238.1"
                     /translation="MSQLFFGYFAGMIAITSMLVVALRNPVYSALSLLVMFFHVAGLF
                     ITLHAEFLAAVQIIVYAGAILVLYLFVVMILNVKQDDRYHSQWRIAGFV"
BASE COUNT          779 a         1074 c         1038 g          870 t
ORIGIN      
        1 accaacgagg aattgtacct ttttcagaaa tttctccgca tggccgtcgg caccaacaat
       61 atcgacagca gcgcgcgcta tggccatgtc aacggcctgc atgccatgca actcgtgcaa
      121 gggacgcatc ggtggaccgt caccttcgac gacatcctgg atgcggatgt cctgatcctc
      181 gtcggcacga acattaccga gacgaatccc atcacgggac tcaaagtcaa agaggcggtg
      241 aagaaacgcc gggcaacgct gatgacgatc gaatcgctgg agccggtcgt cgacacgatg
      301 agcaatatcg ccaatctctc gcaccatcat ttccgcattc cgaccgacga cacgcgccat
      361 gccatcctcg gtttggtgaa ggccgtcgtg gaacagaatt tgacacaccc ggacctcgcg
      421 caacggtatc cggcgtatct caacgccatt acgagcgcgc tgcaacagac ttcttggcag
      481 gacctacagg cggcaaccgg aatcgagccg gattctttta tgagggcggc caaggtagcg
      541 gcgggagcgc gtcgggtcgt tatcttggcc ggacagctct tgctgcggag tgaacatggc
      601 tacagcggat gtgtgactct cctcgatctg cttcttctga taggaaagtt ggatggtcct
      661 ggctgtggtt ttgcgccgct cgccgaggaa aacaacgacc agggcgcggt cgaaatggga
      721 accgtcaccg aatttcttcc cggagcccgc cccgtcgcca gcgacacgga tcgtgagagg
      781 atcgcgaagc aatggaaagg cgaacttccg agcgacagag gagcttccct catcgagatg
      841 ttggatcgag ccgacgcggg atccctcaag gccatgttca tcgtcggaga gaatccggtc
      901 ggaagcctgc ccgcgacgat acatgccgag caatcactgc gcaacctcga tctcttggtg
      961 tgccaggagc tgtttttaac ggagacggcc gccttggccc atgtcgtgtt gccggccgct
     1021 tcgtctctgg aaaaacatgg gacgtttacc aataccgagg gacatgttca ggccgttcgt
     1081 ccctcgatcg agccgatcgg agaaagccgc cccgactggg aagtcttttc cgcgctttca
     1141 atcttgttga actcgccgat ggagtatgcg gagagcaaag agatcctgaa ggaaattcgc
     1201 agcttccttc ccggctatgg ttcgctgggg cccgcgccgt tgccccccaa agttgatccc
     1261 tcggccgtgg atcgctatct cacgaccggc tatcagcggg acctcgcaac caggtaccaa
     1321 cctatcccgc gaacgtcgaa accggatgga accgtgcggc tggacttgac acagagtctg
     1381 ttccattccg ggaaactatc cacacgatcg aaggggttgt tacaactcga agggagcggc
     1441 cggctccgta tcagtccgct tgacgccgcg cgctttgcct tgtcggatgg ggatcgagtc
     1501 cgcctttcca gcacgtccgg ggaaatgacg accgaggtca agataatgga gcgcgtccca
     1561 caaggaacgg cgtggttccc gtatcacttt ggtcaagctg ccgtccgact atttgaatgc
     1621 gccgtggacc cgatcacccg tgtaccgtca tttcggacag cgacggtgtc cgtcatgaag
     1681 gtggcgtgat gactctcttc atccctgatg gttatgctaa tcgaggagtc caattgtgac
     1741 tgaattcggg ttacgtctcg ctatctcgct gacccagatc gccggagtca tgggcatcgt
     1801 ggtcgtcacg gtgctcatcc tcactctggc ggaacgaaaa gttctagggt ggatgcagga
     1861 ccgcatgggt ccgatggaag tggggcccta cggcattctc cagccgtttg cggatgccat
     1921 caagctcttc tttaaggaag acattatccc tgccggcgcc aacaagttcc ttttcaccgt
     1981 tgcgccgatt ctttgtttaa ttcccgcgtt catcggattt gccgtcattc cgtggggtcc
     2041 caaccagacg tttgagatcg acggcatcac cgtaaggccg tttgtgatca gcgacatcaa
     2101 tatcggcatt ctgtacatct tagcgttcgc ctcgctcggg gcttacggca tcatcctggg
     2161 gggatgggct tccaacagca aatattcatt gctcggcggg ctacgatcgg cggcacagat
     2221 catcagttac gagctcaacg tcggtttgtc catcgtcgga gtgttgattt tggccggttc
     2281 actcagcctt gtgaaaatca ccgatgccca ggccggaggc ttttggaatt ggtatctctt
     2341 tgcgttaccg gccccgcaaa tttttgcgtt tgtcgtctac gtgatctcat cggtggctga
     2401 aaccaaccgg gtcccgttcg atctgccgga agcggagagc gaactcgtcg caggcttctt
     2461 cactgaatac agcggcctca gattcgcgtt ctttttcctc gccgagtacg ccaacatggt
     2521 gttagtgtcc tgcgtagccg ccgcgctgtt tctcggcgga tggaacgcac cgtaccctgg
     2581 aacgattatg gcgctcatcg gtttgccgtc cttggcctgg gtcgagaaca cgatgtggtt
     2641 tgcggtcaag acctactctt tcttgttcct cttcttctgg cttcgagcca cgttaccgag
     2701 actgcgatac gatcagctga tgagattcgg ctggaaggta atgctgccca tcgcgttggg
     2761 taacatcgtc gtgacgtccc tcgcggtatt tttctaccaa cagatgaagt agtaggcgag
     2821 gcctatatga tatctacggc gaccaccaag cgtttcaacc tgtacgaatg gttcaaaacg
     2881 atcacattct acgagattct cgtcggcatg aaggcgacct tgtcgcatct gctccattac
     2941 cgtcccgtaa ccgtgcaata ccctcatgaa aaacgcacac tgccggacaa ttaccgaggc
     3001 atgctcgcgc tgctccgata cgatgatgga accgagaagt gcgtgggatg cgatctctgt
     3061 gaagcagcct gtccgtctcg cgtcatccgg gtcgtcagcg ccgaagtgcc gggtgaaccg
     3121 actaagcgtt actccaaaga atattacatg gacatgaccc gttgcttgtt ctgcgggatg
     3181 tgcgtggatg cctgccctgt cgatgcgctg ggcatgacga gagagtttga gtgggcggtg
     3241 tacgacaagc gccagctcca cctggacaaa caacaattgc tcgcgatcgg cgaccgctca
     3301 tttccagtcc gtgagaaacg cctggagctg caacatccaa acgtcgcgtt cttcaatgtg
     3361 gcattcaagc acgtgccacc aaaaccgaac tgactctcaa agaattctca tcggacgagc
     3421 gatgactgca acaccggagc attagtctga ccctgatcgc cctctaactc aggaaccgtc
     3481 tcatgtcgca gctgtttttt ggatacttcg ccgggatgat tgccatcacc tccatgctgg
     3541 tggtcgcgct gagaaacccc gtctacagcg cgctttcctt actcgtcatg tttttccacg
     3601 tcgcggggct cttcatcacg cttcatgccg aatttcttgc ggccgtgcag attatcgtct
     3661 atgccggagc cattcttgtg ctgtatctgt tcgtcgtgat gatactcaac gtcaagcaag
     3721 acgaccgtta tcatagtcaa tggcggatcg cgggatttgt c
//
LOCUS       DATMTI010000255         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_688655, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000255 DATMTI010000000
VERSION     DATMTI010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688655"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VN039_04300"
     CDS             <1..766
                     /locus_tag="VN039_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HWV45239.1"
                     /translation="RTEEIAKTSRLVSKITGMMVQPNKAIVGANAFAHTSGIHQDGLL
                     KEKTTYEIMRPESIGLVESQMVMGKLSGRHAFRQRLEELGYKLGETEVNHAFERFKKL
                     ADQKKEIFEEDLEVIVSEELSKMAERIALKALQVSSGTDRVPTATVELEIDGTPIVQT
                     GTGDGPVDAVYRTIAAMTRTKSKLLMYVVKAITGGTDAQGEVSVRVQEDGRTVTGHGA
                     DTDIITASARAYISALNKLAYLATKQAQGEQKVNLI"
     gene            832..1194
                     /locus_tag="VN039_04305"
     CDS             832..1194
                     /locus_tag="VN039_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV45240.1"
                     /translation="MFKVTLADRPEFLAGDETRLREIFHPAKHQLKLNYSLAHGTLPS
                     GRRSKRHVLASSEVYYFISGRGRFTIDDHVTPIEAGTTIYVPPGGRQFVENTGETDIE
                     FLCFVDPAWRIEDETILE"
     gene            1230..2303
                     /gene="leuB"
                     /locus_tag="VN039_04310"
     CDS             1230..2303
                     /gene="leuB"
                     /locus_tag="VN039_04310"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWV45241.1"
                     /translation="MKAKIAVLAGDGVGREIVPEAVKVLKIIAEKYGHSFEFVAADIG
                     GQAIDKAGVPLPKDTLALAKQSDAVLLGAVGGPRWENLEYSLRPERALLGIREALGLY
                     ANLRPAKLYSNLVDASTLKREVVEGIDILVIRELTGGIYFGKPKGIEKLSNGEERGVN
                     TEVYSTEEVRRIAKVAFEAARKRRKKVTSVDKANVLESSELWRKVVIEVHASYPDVEL
                     GHIYVDNAAMQLVRNPRQFDVLLCNNMFGDILSDEAAMLTGSIGMLPSASIGAKVGLF
                     EPIHGSAPDIAGKNVANPIATISSAAMMLSYAFQLEKEAEAIEQAIVKTLDLGYRTKD
                     IQSPGAKIVGTMEMGEAIVRNLN"
BASE COUNT          594 a          614 c          761 g          514 t
ORIGIN      
        1 ccgaaccgag gaaattgcaa agaccagccg tttggtcagc aagatcacgg ggatgatggt
       61 gcagcccaat aaggcgatcg tgggggcgaa cgcctttgcc catacatcgg gtattcatca
      121 ggacggcttg ctcaaagaga agacgaccta tgaaattatg cgaccggaat caatcgggtt
      181 ggtcgaaagc cagatggtga tgggcaagtt gtccgggcgt catgccttcc ggcagcgatt
      241 ggaagagttg ggttacaagc tcggcgagac agaggtcaac cacgccttcg agcggttcaa
      301 aaagcttgcc gatcaaaaga aagagatctt cgaggaagat ctcgaagtca ttgtttccga
      361 agaattgtca aagatggccg agcgcatcgc cttaaaggcg cttcaggtct cgagcggaac
      421 ggatcgggtc ccaaccgcca cggtcgaact ggaaatcgac ggcacaccca ttgttcaaac
      481 cggaaccggt gatgggccgg tggatgccgt gtaccgcacc attgccgcca tgacacggac
      541 caagagcaag ttgctgatgt atgtcgtaaa ggccatcacc ggtgggacag atgcacaggg
      601 agaagtgtcg gtgcgcgtgc aagaggatgg tcggacggtc acagggcacg gcgccgatac
      661 tgatatcatc acggcatcag ctcgggccta catcagcgcc ctgaataagc tggcctacct
      721 ggcgacgaaa caagcgcaag gcgagcagaa ggtgaacttg atttgactcg gatcctcacg
      781 gtacagtttc gccttctgtc gtgaggtctg ttggattgag agggcttgtc cgtgttcaag
      841 gtgacgctgg cggatcgccc cgagtttctg gccggggacg agactcgctt gcgggagatc
      901 tttcatcctg ccaagcatca gctcaaactg aattatagtc ttgcccacgg gacgttaccg
      961 tccggtcgac gctcaaagcg ccacgtcttg gcttcgtcag aagtctatta cttcatttct
     1021 gggcggggcc gcttcacgat cgacgaccat gtcacgccga tcgaagccgg aacgaccatc
     1081 tatgttccgc caggaggtcg gcagttcgtc gaaaataccg gggagaccga tattgagttt
     1141 ttgtgtttcg tcgaccccgc ctggcggatt gaagatgaaa cgatactgga ataggccgaa
     1201 agccgattga gttattgaaa ggagccagag tgaaggccaa gattgcagtg ctggccggtg
     1261 acggagtcgg tcgcgagatc gtccctgaag ccgtaaaggt cttgaagatc attgctgaga
     1321 aatacggaca ctcgttcgag tttgtcgcag ccgatattgg cgggcaggcg atcgataaag
     1381 cgggtgtgcc gctgccgaaa gacaccttgg cgctggccaa gcaaagcgat gccgtcttgc
     1441 tgggcgccgt cggggggcct cgatgggaga acttggagta tagtctgcgg cccgaacgcg
     1501 cattgctcgg catccgcgag gccttagggc tctatgccaa cttgaggccg gcaaagctct
     1561 attccaattt ggtggatgct tccacgttga agcgcgaggt cgtcgagggg atcgacatcc
     1621 tcgtgatccg cgagctcacc ggcggtatct atttcggcaa accgaagggg atcgaaaagt
     1681 tgtccaacgg tgaagaacga ggggttaata cggaagtcta ttccacggaa gaagtccggc
     1741 ggatcgccaa ggttgcattc gaggcggcac ggaaacggcg caagaaggtc acttcggtcg
     1801 acaaagcgaa tgtgttggaa tcatccgagc tttggcgcaa ggtggttatt gaagtccatg
     1861 cgtcctatcc ggacgtcgaa ctgggacata tctacgtgga taacgccgcg atgcagctgg
     1921 tgcggaatcc gcgacaattc gacgtgctgc tctgcaacaa catgttcgga gacattttga
     1981 gcgatgaagc ggcgatgctg accggttcga ttgggatgtt gccgtcggcg agtatcgggg
     2041 ccaaggtcgg cctctttgag ccgattcatg ggagcgctcc ggacattgcg gggaaaaatg
     2101 tcgccaatcc cattgcgacg atctcgtctg ctgccatgat gctatcgtat gcctttcaac
     2161 ttgagaaaga agcggaagcc atcgaacagg cgattgtaaa aactctcgat cttggatatc
     2221 ggaccaaaga tattcagagt cctggagcga aaatcgtcgg cactatggag atgggtgagg
     2281 caatcgtccg aaacttgaat tagtcagcgg tcctgaacaa tggcggccat tctttccaca
     2341 tggatgatca gaacactcgt cggcaccggc gagtccggtt atacgggaga cggcggacct
     2401 tcaatacagg cccgtttgaa cgagcccaag ggcgtgacga tcgattacca cggcaatgtc
     2461 tatgtagccg attcagaaaa tca
//
LOCUS       DATMTI010000256         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_816576, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000256 DATMTI010000000
VERSION     DATMTI010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816576"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VN039_04315"
     CDS             <1..223
                     /locus_tag="VN039_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45242.1"
                     /translation="VLDLIMPGMPGIEVLRALRDKGYPGGIIIMTGSHNEELLREAWS
                     LGSQEILIKPVDLERLLTAIQLVLVCREC"
     gene            235..>2194
                     /locus_tag="VN039_04320"
     CDS             235..>2194
                     /locus_tag="VN039_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HWV45243.1"
                     /translation="MSIRKYLLHSCRIGLLAASIAHTWGVSALPAQENVTNIQHTLSA
                     ELILAAHELVANDQIKLEVEPEVTSVTFTLAPTLHVESIAMQTHSVSGGQGNQDEVVP
                     FTSGYSSENTVQHVVVSLPKGHGRRVTLIMSYRGQINDPPREPRHLRFVTPSETAGYI
                     GLDGVYLSGESQWYPDVIGSFSTYRVTAQIPQGWTVVASGRKESETTNSGKTSSTWIV
                     QNQSEAFTLVANRFVTTSREWKSPTGQRVELQTHFLPDNAGLADEYLDATARYLDVYV
                     RILGNYPFDKFAVVENFFASGLGLPSFTLLGSGSIRRHYVQPYALGHEIVHSWIGNSV
                     FNRDNHGNWVEGMTTYLANYYWHEVVKDVSQAFEQRRMMLDGYNLYVRPETDYAVSQF
                     LRKHDEKDNAIGYHKSAFVFHLLRQEIGDEPFWRGLKTFVSGYRNHPADWRSIEEVFS
                     RESGQDLRWFFEQWVEQPGAPLVSLRDVRARHVKGEGGRETWRLTVQIDQAGKPFRMA
                     IPVRIVMKESTDIKPIELNLSQASVTEFVLPDQPLRVELDPELMTFRRLTRRQLPPML
                     NGYATDRQKTVVLAFSDPASPLQQIVSRITDHEQTGPHKTTVVSLKDNSLPHDGSILI
                     LAGADERQAVQSVVQESCGDQIALGEGGG"
BASE COUNT          501 a          578 c          623 g          492 t
ORIGIN      
        1 cgtgctcgac ttaatcatgc ccggaatgcc ggggatcgaa gttcttcgcg ccttgcgtga
       61 caagggttat ccgggcggca tcattatcat gaccggaagt cacaatgagg agttgcttcg
      121 agaagcttgg agcctaggct ctcaggagat ccttataaaa ccagttgatc ttgaacgcct
      181 cttgaccgcg atccagcttg tactcgtctg tcgcgagtgt taaaacgctt cctgatgtcc
      241 atcaggaaat atcttcttca ctcttgccgg atcggtctgc tggcggcctc catcgcacac
      301 acatggggtg tgtcggccct gcctgcccag gagaatgtca caaacattca gcacaccctc
      361 tcagccgagc tgattctcgc tgctcatgag ctggtggcca acgatcagat caaactggag
      421 gttgagccag aggtcacgtc ggtcacgttc acgcttgcac ctaccctgca tgtcgagtcc
      481 atcgccatgc aaacgcattc ggtctccggc gggcaaggaa accaggacga agtcgtccca
      541 ttcacgagcg ggtattcttc cgagaacacg gttcagcacg tcgtcgtctc tcttccaaaa
      601 gggcatggac gcagggtgac gttgatcatg agctatcgtg gccagatcaa tgaccctccg
      661 agagagccgc gccacttgag atttgtgaca cccagtgaga cagcgggata tatcggcttg
      721 gacggcgtgt acttaagcgg ggaaagccaa tggtaccccg atgtcatcgg ctcattcagt
      781 acctatcgag tgactgctca gattccacaa ggctggacgg tggtggcctc cggacgcaag
      841 gaaagtgaga cgaccaactc ggggaagacc tcttcaacct ggatcgttca gaaccagtct
      901 gaggcgttta ctctcgttgc caacagattt gtgacgacat cgcgggaatg gaaaagtccg
      961 acagggcaac gagtcgaact ccagacccat ttcttgcccg acaatgcggg tttggcggat
     1021 gaatatctcg atgcaactgc aaggtatctc gacgtatatg tcaggatcct agggaactat
     1081 cccttcgaca aatttgcagt ggtcgagaat ttctttgcaa gtggtcttgg tctgccgtcg
     1141 ttcactctac tcggcagcgg cagcatcagg cggcactatg tccaacccta tgccttgggt
     1201 cacgagatcg tccattcctg gatcggaaat tccgtgttca atcgagacaa tcatggcaac
     1261 tgggttgaag ggatgaccac ctatttggcg aattactact ggcacgaggt ggttaaggat
     1321 gtttcgcaag ccttcgaaca aagacgcatg atgctggacg ggtacaatct ctatgtgagg
     1381 cctgaaacag actatgcggt ttcacagttt ctgaggaagc atgacgagaa agataatgcc
     1441 atcggctacc acaaatcggc ctttgtgttt catctccttc ggcaagaaat cggagacgag
     1501 ccgttctggc gcggcttgaa gaccttcgtc agcggctatc gcaatcaccc ggcggactgg
     1561 agatcgatcg aggaagtctt ttctcgagaa agcggtcagg acttgcggtg gtttttcgag
     1621 caatgggtcg aacagcccgg tgccccactt gtatccttgc gtgatgtccg cgctcgtcac
     1681 gtgaagggag agggcggcag agaaacttgg cggttgacgg ttcagattga ccaggccggg
     1741 aagccgtttc ggatggcgat ccccgtccgc atcgtgatga aggaatcgac ggacatcaag
     1801 ccgattgagc tgaatctctc gcaagcgagc gttacggagt tcgtccttcc cgatcagccg
     1861 ctgcgggtgg agcttgatcc cgagctgatg acgtttcggc ggctgacgag acgtcagctg
     1921 ccgcccatgt tgaacggcta tgcgacggat cgacaaaaga cggtggtcct ggcgttttct
     1981 gacccagctt cgccgttgca gcagattgtg tcccgcatca ccgatcatga acaaacggga
     2041 ccgcataaaa ccacggtggt ctctctaaaa gacaattccc tcccccacga tggatcgatc
     2101 ctgatactgg caggagccga tgaacgacag gcggttcaat cagtggtgca ggagtcttgc
     2161 ggtgatcaaa ttgctctcgg agagggggga ggaa
//
LOCUS       DATMTI010000257         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_180231, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000257 DATMTI010000000
VERSION     DATMTI010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180231"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="VN039_04325"
     CDS             complement(<1..1824)
                     /locus_tag="VN039_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWV45244.1"
                     /translation="MNEAETRAEHVDPALKAAGWGMVEGSRVLREYPITLGRIEGLGR
                     RAKPLIVDYVLVYRNTKLAVIEAKAWDEQLTEGVGQAKDYAGKLAIRFAYATNGQGIY
                     GIDMETGKEGEVTQYPSPDQLWNVTFSKQNEWRDRFAAVPFEDKGGSHPSRYYQDIAV
                     ERVMQAMAENKSRILLTLATGTGKTFIAFQIAWKLFHSRWNLSREPSCRPRILFLADR
                     NILADQAYNAFSAFPEDALVRIEPDDIRKKGKVPKNGSLFFTIFQTFMSGPGDTPYFG
                     EYPPDFFDFIVIDECHRGGANDESNWRGILEYFAPAVQLGLTATPKRRDNVDTYAYFS
                     DPVYIYSLKDGINDGFLTPFKVKQISTTLDEYVYTPDDTLVEGEIEAKKRYTESDFNK
                     IIEIKEREKKRVEIFMGQINQQEKTLVFCATQDHALAVRDLINQMKTSTDPNYCQRVT
                     ASDGALGEQHLRDFQDNEKTIPTILTTSQKLSTGVDARNIRNIVLMRPIHSMIEFKQI
                     IGRGTRLYDGKDYFTIYDFVKAHHHFSDSEWDGEPIDPEPPVRPYEPKVGRHGGAGRI
                     SEPPGRYTKIKVKLADGKARTIQHMMVTTFWHPDGTPMSAQQ"
     gene            complement(1821..>2390)
                     /gene="rhuM"
                     /locus_tag="VN039_04330"
     CDS             complement(1821..>2390)
                     /gene="rhuM"
                     /locus_tag="VN039_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HWV45245.1"
                     /translation="TSVDYRPDMELSQQFFATVQNKMHWATHGQTAAEVIHRRADATK
                     PFMGLTTTRPGGIVRRDDVSIAKNYLTEDELHALNRIVTAYLEFAELQALNRRPMTMR
                     DWITKLDEFLQLSGRELLNHAGKISAESAKAKAEQEYDRYRALIDAQSRAVDADFEQV
                     AKQIQKMPKLRVRVERQQKKPKTSKKKLP"
BASE COUNT          456 a          684 c          650 g          600 t
ORIGIN      
        1 ttgctgcgcc gacatcggcg tgccgtccgg atgccagaag gtcgtcacca tcatgtgctg
       61 gatcgttctg gcttttccat cggccaactt aaccttgatc ttggtatatc gccctggcgg
      121 ttcactaatg cgtcctgccc ctccatgacg acccactttt ggctcatatg gcctgacagg
      181 tggttctgga tcaatcggtt ctccatccca ttcagaatca ctgaagtggt gatgcgcctt
      241 cacaaagtcg taaatcgtga agtagtcctt tccgtcatag agcctggttc cccgtccgat
      301 gatttgcttg aactcgatca tcgaatgaat cggacgcatc aggacgatgt tgcgaatatt
      361 gcgagcgtcc acaccggtcg agagcttttg ggaagtcgtc agaatcgtcg ggatggtctt
      421 ttcgttgtct tgaaagtcac ggagatgctg ttcgccgagt gccccgtcgc tggccgtcac
      481 gcgctggcag tagttcgggt ctgtgctggt cttcatttga ttgatgagat cgcgtaccgc
      541 gagggcgtgg tcctgtgttg cacaaaacac aagcgtcttc tcctgctggt tgatctggcc
      601 cataaaaatc tcgactcttt ttttctctcg ttccttgatc tcgatgatct tgttgaagtc
      661 gctctccgta tagcgtttct tcgcttcaat ctcgccttcc acgagcgtat cgtccggcgt
      721 atagacatac tcatcgagcg tcgtggagat ctgcttcacc ttgaacggcg tgaggaagcc
      781 gtcgttgatg ccgtccttca gcgaatagat atagacgggg tcactgaaat aggcgtaggt
      841 gtctacgttg tctctgcgtt tgggtgtggc agtcaggccg agctgcacgg ctggggcaaa
      901 atattcaagg ataccgcgcc agttgctctc gtcgttcgcg ccgccacgat ggcactcgtc
      961 gatgacgatg aagtcgaaga agtccggtgg atactcaccg aaatagggtg tgtcgcctgg
     1021 tccactcatg aaggtttgga aaatggtgaa gaacaaacta ccgttcttcg gcaccttgcc
     1081 cttcttccga atatcgtccg gctcgatcct caccagcgcg tcttccggaa aggcagagaa
     1141 cgcgttatag gcctgatcgg cgaggatgtt acggtcggcc aggaagagaa tgcgcggacg
     1201 gcatgacggt tcgcggctca ggttccaacg gctgtgaaag agtttccagg caatctgaaa
     1261 ggcgatgaag gtcttgccgg taccggtcgc gagggtgagc aggatgcgcg acttattttc
     1321 cgccatagcc tgcatcacac gctcaacggc aatgtcttga taatagcggc tggggtgtga
     1381 cccgcccttg tcctcgaagg ggacggcagc aaagcgatcg cgccactcgt tttgcttgga
     1441 aaaggtgacg ttccagagct ggtccggcga gggatactgc gtcacctccc cttccttgcc
     1501 ggtttccatg tcgatgccgt agatgccctg cccgttggtg gcataggcga agcgaatggc
     1561 cagcttcccc gcatagtcct tcgcctgccc taccccttcg gtcaactgtt catcccaggc
     1621 cttggcttca atcacggcaa gtttggtgtt gcgatacacg agtacataat cgacgatcag
     1681 cggtttcgca cgccgtccca aaccttcgat ccggccaagc gtgatgggat actcgcgcaa
     1741 cacgcgactc ccctcgacca taccccaccc cgcagccttg agggcaggat caacgtgctc
     1801 ggctctggtt tccgcttcgt tcatggcaac ttcttcttcg acgtcttcgg cttcttttgt
     1861 tgccgctcga cacgaactct gagcttcggc atcttctgaa tctgtttcgc aacttgctca
     1921 aagtccgcat ccacagcgcg cgattgcgca tcgatgagag ctcggtaccg gtcgtattcc
     1981 tgctcggcct tcgccttggc ggattcggcg gaaatctttc ccgcatggtt cagcaactcg
     2041 cgcccggaca actgtagaaa ttcatccagt tttgtaatcc agtcgcgcat tgtcatcggt
     2101 cgtcgattga gcgcctggag ttcagcgaat tccaggtagg cagtcacaat acgattcagc
     2161 gcgtgcagct cgtcctcggt gaggtaattc tttgcaatag agacgtcgtc cctccggaca
     2221 atcccaccgg gacgcgtggt cgtcagcccc atgaacggct tagtcgcgtc agcccgccgg
     2281 tggatcactt ccgccgccgt ctgcccatgc gtggcccagt gcattttgtt ctgcaccgtc
     2341 gcaaaaaact gctgcgacaa ttccatatcg ggccggtagt ccacactcgt
//
LOCUS       DATMTI010000258         1935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_688752, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000258 DATMTI010000000
VERSION     DATMTI010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688752"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..484)
                     /locus_tag="VN039_04335"
     CDS             complement(173..484)
                     /locus_tag="VN039_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45246.1"
                     /translation="MRHTAMVMPTQHMQQGMRLYCAFHQFGPAHVVKVHHPRVIPPVV
                     VELGELVGLMYRSDKGQPGQPLTYIHRMEDPPRLVSNIEGTQLYIVGGSYRVTPRGIE
                     G"
     gene            complement(546..1649)
                     /locus_tag="VN039_04340"
     CDS             complement(546..1649)
                     /locus_tag="VN039_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWV45247.1"
                     /translation="MNQTMNGAQSSGSCRCNTSPGSFGGNLKLSKKQAPPMQRYTSAP
                     LSAPKISLGHIPTGWRGTQKTVEHVQALIRAGAKDFYVRQKAIDILLEKHVKPKDYLA
                     EIKALLEWVQQHIRYTKDTYQVEVLHTARRMLELRAGDCDDMTILLGSMLEAIGHPVR
                     LVLSGQDPLRQDLFTHIYLEVFHKGRWIPLDATMPYPMGWAPKTLVKKIIPIKRRSTM
                     MAEDMELQGIGAAGAVPDWLKGLIRTVRREALQPKDARIKSLWDLLRQRQLLHRSRWL
                     KAVLQRMWSRGLSARPHPRTARRIVKRLRRWGILPPKLVGAAAYRPVGTRPVTRAAVQ
                     AVRPVALKPVASLRPATMQPVRPVQMQPAGAKR"
     gene            complement(1686..>1935)
                     /locus_tag="VN039_04345"
     CDS             complement(1686..>1935)
                     /locus_tag="VN039_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45248.1"
                     /translation="IVATEEFHFLMNRFVRFKVSDVTIPGPQVVVTELYGGDILQGKV
                     VDITKGGTQEQAFIVVKVEAMKDFLILPAEKVIGMTDQ"
BASE COUNT          397 a          578 c          481 g          479 t
ORIGIN      
        1 ctgatacaac gtgccgtctt cgcccaagaa catggcttcc gcacctgatg cactggggtt
       61 cacgatcatc aactcatcca atccgagttg ttggttcttt tccatgtcgc tccctctgct
      121 tgtgtaggcg aatcggccat atgcaaattg ctcaacatga attccgatcg ctctagcctt
      181 cgatccctcg tggagtgacc cggtaacttc cccccacgat atacagttgc gttccttcaa
      241 tgttgctaac cagacgcggt ggatcctcca ttcggtgaat gtaggttaga ggttgacctg
      301 gttgtccttt atcggagcga tacatcagcc ccaccaattc gcctagttcc accacgaccg
      361 gcgggattac tctgggatga tgaactttca cgacgtgagc cggtccgaat tgatgaaacg
      421 cacagtagag cctcattcct tgctgcatgt gttgagtcgg catgaccatg gcggtgtgcc
      481 tcatcagcga tccgttatga acgctgtccc catgggaaca tgtttcgcat cgacaactca
      541 tttccctatc gctttgctcc cgccggttgc atctgcactg gtcggaccgg ttgcatggtt
      601 gcaggccgca acgacgcgac tggtttgagc gccactggtc gaaccgcctg aacagcagct
      661 cgtgttacag ggcgagtgcc taccgggcga tacgccgctg cacccactaa cttcggcggc
      721 aagatgcccc accgtcgcaa tctcttcacg atccggcgcg cagtgcgcgg atggggccgt
      781 gcggacagtc ctctactcca catacgctgc agcactgcct tgagccatcg gcttcgatgc
      841 agcaattgac gttgccgtag caaatcccac aaggacttta ttcgcgcgtc tttgggttgt
      901 agggcctctc tccgcaccgt cctgatcaat cccttcagcc aatccggaac cgccccggca
      961 gcgccgatgc cttgtagttc catgtcctca gccatcatgg tggacctcct cttgatcggg
     1021 atgatttttt tcaccagtgt cttgggcgcc cagcccatcg gatagggcat ggtggcgtcc
     1081 aacggaatcc agcggccttt gtgaaacacc tctagataaa tatgagtaaa aaggtcctgc
     1141 ctgagtgggt cttggccgct gagcaccagc cgtactggat gccctattgc ctccaacatt
     1201 gaccccaaca ggatggtcat gtcgtcgcag tcaccggctc gtaactcaag catgcgtctc
     1261 gcggtatgga gcacctccac ctgataggtg tccttggtat aacgaatatg ctgctggacc
     1321 cattcgagaa gagctttgat ttcggccagg tagtccttgg gcttgacgtg cttctccaac
     1381 aaaatgtcga tggccttttg gcgaacataa aaatctttcg cgccggctcg gatcagcgcc
     1441 tgtacgtgtt caaccgtttt ctgggttccg cgccaaccag tcgggatatg accgagagag
     1501 atcttcggcg cgctgagcgg cgccgatgta taccgctgca tgggcggagc ttgcttttta
     1561 ctcagcttca gattcccgcc aaacgaacca ggtgaggtgt tgcatcgaca cgacccactc
     1621 gattgcgcgc cattcatcgt ttgattcacg tgtagttccc tcacgatctc catgcgacga
     1681 ttcgctcact ggtccgtcat gccaatcact ttttctgcgg gaagaatgag aaagtccttc
     1741 atggcttcca ctttcacaac aatgaacgcc tgctcctgcg tcccaccctt cgtaatatca
     1801 acaaccttcc cttgcaggat gtcccctcca tacagttcag tgacgaccac ttgcggacca
     1861 gggatggtga cgtcactcac tttgaagcgt acgaaccgat tcattaaaaa atggaattct
     1921 tcggtagcca caatc
//
LOCUS       DATMTI010000259         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_392275, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000259 DATMTI010000000
VERSION     DATMTI010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392275"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VN039_04350"
     CDS             <1..708
                     /locus_tag="VN039_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45249.1"
                     /translation="IENKKAIVAAGFGYQIGAVSTIAVKVYDAESGDILSNEIYELAV
                     KESDGVRSNRGPRIFAGGVGLGATDLSNFVLRVYDANTGVFQWEGRLNLVQPDWKAGG
                     KVVSTTLPRRATVTRVHIGEATIEQPVFLLRAMNAATGVLVWEDEFTTVRTRIPRAQW
                     IADRSIQPDSGPLASSHTFNFRIRMYDPSGKKVLWEDQLSQLGSDEKPEQSHSDQADI
                     LPAWPNLPPEESAAGAI"
     gene            complement(719..2134)
                     /locus_tag="VN039_04355"
     CDS             complement(719..2134)
                     /locus_tag="VN039_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45250.1"
                     /translation="MYRRNIRHILIPLAIGLASVIGYNLFLRPVLPSQTVQLTETIEP
                     VAPPPAPPQRRSPESEAKSVRVLDQSVIPPTPHEALLEAIRDEIEKHNLSLAETKLNE
                     LPSAFLSDAKAKPFVATLWNNLGLQQERLNGTQTSVKAFKRAADLDDSNSVILMNLAH
                     AYWEQRDRRLNAEFLIKLMRVAPEEPFPHLAMADLLQEQDELQEAAKHLEQAADRARQ
                     DPALQSYLAAVTAKVRRTQSVESHMTARSSTHFIVKFDGEEDQSTWTSVLEILEEAYR
                     EIGQKFGHFPSKPIMVVLHAKDSFQAATGSPAWADGLYDPTLGRIQIPTQGATTDRKW
                     LASVLRHEYVHALLHDRLGASSGALPTWLNEGLAMQLAGDAWPELDQAMSGDVKVIPL
                     IYLEGSWGALPASTSTVAYLEANSAARYLIERWGMAGVDELLNALQAKSSITAALQNK
                     LFVSYEQFHRQWLESFERSRS"
     gene            2269..>3713
                     /locus_tag="VN039_04360"
     CDS             2269..>3713
                     /locus_tag="VN039_04360"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWV45251.1"
                     /translation="MRTPTGSSPHTPFQEWLDRIARPIEFASRDDCAHLKAVTNLSSF
                     ISSQVLSALCQEVYPKAIEARLISLRDLFIDFQPALPFVEQRRRLQAAVLLIKSLRAI
                     DQQKLIRLKDLPTQASCHSEVTGAGRSDLWNLPIRFVKGVGPKRTNVLQRMHIETVED
                     ALWTMPWRYEDRSVMTPIGNLVPGRIASICGAIGTCEAKRTRTRRLSVVEVGVEDQTG
                     RLQVVFFNQPYVEEILTVGTRVMMSGRVLSGRQGWMVPRMDVTQYEIIGEDTESTLHV
                     GRIVPIYHETKGWTSRQMRVLVKNLLADHGVALLDHLPVPLRARQRLIPIQEALHDAH
                     FPKIGTDLQLLGRGKTAAHRRLAFEELLLLQLALATRHRSVYEEPKELRFNPRTPLLQ
                     QLSRLMPFRLTTAQDRVVREIFRDMISSRPMNRLVQGDVGSGKTAVALHALVLACGSG
                     YQAALMAPTEILAEQHYRNLSRTLQTLGLHAT"
BASE COUNT          822 a          975 c         1069 g          847 t
ORIGIN      
        1 atagagaaca agaaggcgat tgtagcagcg ggattcggtt accaaatcgg cgccgtctca
       61 acgattgcgg taaaggtgta tgacgctgaa tcgggcgata tcttgtccaa tgaaatctat
      121 gaactagccg tcaaggaaag cgacggcgtg agatcgaacc gagggccgcg tatttttgcc
      181 ggtggagtcg gacttggcgc taccgatctt tccaacttcg tgttgcgcgt ctacgacgca
      241 aataccggag tgtttcaatg ggaagggcga ctcaatcttg tgcagcctga ctggaaggcg
      301 ggaggaaagg tcgtatcgac cacactacct cggagagcaa ctgttacgag ggtacacatt
      361 ggtgaagcga cgatagagca gccggtgttt ttgctgcgtg caatgaacgc ggcgaccggc
      421 gtgcttgtgt gggaagatga gtttacgacc gtccgtacac gaattccgcg agcccagtgg
      481 attgcagatc gatcgatcca gccggacagc ggtccacttg cgagttccca tacctttaac
      541 ttcagaattc gcatgtatga tcccagcgga aagaaggttc tgtgggagga tcagctgtct
      601 cagctggggt ctgatgaaaa gcctgagcag tcgcacagcg atcaggctga catacttcct
      661 gcctggccta acctaccgcc agaagagtcc gcagcaggag cgatctaagc agcgaacgtt
      721 acgaccgact ccgttcgaaa ctctcgagcc actgccgatg gaactgctcg taggaaacaa
      781 acaacttgtt ttgaagtgca gctgtaatcg acgatttggc ttgtaaagca ttcagcaact
      841 catcgacgcc ggccataccc catcgctcaa tcaagtagcg tgctgcagaa ttcgcctcta
      901 agtaggccac cgtcgacgta ctggccggca gtgcccccca tgagccttcg agatagatca
      961 aaggaatgac tttgacgtct ccgctcatgg cctgatcaag ctcgggccac gcatctccgg
     1021 ccaattgcat ggccaggcct tcgttcagcc aggtcggcag cgctccgctg cttgctccta
     1081 agcgatcgtg gaggagagcg tggacatact cgtgacgaag cacgctcgcc aaccacttcc
     1141 gatccgtggt cgccccttga gtgggaattt gaatgcgtcc aagggtgggg tcatagagac
     1201 cgtcggccca agccgggctc ccggtcgccg cttgaaacga atccttagca tggaggacga
     1261 ccatgatcgg tttcgaggga aaatgaccga acttctgccc aatctctcga taggcttctt
     1321 caagaatctc cagaactgag gtccaggtgc tttggtcttc ctccccgtcg aacttcacga
     1381 tgaagtgagt actgcttctt gcagtcatgt gagactcaac cgactgagtg cggcgaacct
     1441 ttgccgtgac ggcggcgaga taggattgta atgccgggtc ttgccttgct cggtctgctg
     1501 cctgctccaa atgttttgcg gcttcctgca gttcatcttg ttcttgtaac aagtcggcca
     1561 tggccaagtg gggaaacggt tcctctgggg ctaccctcat caatttaatc aggaattctg
     1621 cattcaacct gcggtctcgc tgttcccaat aggcatgggc aaggttcatg aggatcacgg
     1681 aattcgagtc gtcaagatcc gcagctcgct tgaacgcttt taccgaagtt tgagtgccat
     1741 tgagtcgttc ctgctgcaac ccgagattgt tccacagggt tgcgacgaac ggcttggcct
     1801 ttgcgtccga caggaaagcc gaagggagct cgttcagttt cgtttcggca agcgacaagt
     1861 tatgtttttc gatctcatcg cgaatggctt ctaacaaggc ctcatgcggg gtaggcggta
     1921 tcacgctttg atcgagtacg cgaaccgatt tcgcctcact ttctggcgat ctgcgctgcg
     1981 gcggagcggg aggaggagcg accggttcga tggtttccgt gagctgcacc gtctgtgacg
     2041 gcaggacagg cctcaagaat aggttgtatc cgatcaccga agctaagccg atggccagcg
     2101 gtatcagaat atgtctgatg ttgcgacgat acatgtgtgc ttgataccga gtgtagcatc
     2161 ctaagaaaag gtgaccaagc aatccggcaa aacagactgt gcaccgtgtc acgaggtttg
     2221 tctggttcat tgagcgttga gaaacgctgt gttacgatcc tccaccatat gcggactccc
     2281 actggatcga gtccacacac gccttttcag gagtggttgg accgcatcgc gcgaccaatc
     2341 gagtttgcaa gccgagatga ttgtgcccac ctcaaggctg tcacaaatct gagttcgttt
     2401 atctcctcac aagtcttgtc tgccctttgc caagaggttt atcccaaggc tattgaagcc
     2461 cgcctgattt cgttgcggga cctgttcatc gattttcagc cggcgctccc cttcgttgaa
     2521 caacgtcgac gattgcaagc ggctgtgttg ctcattaagt cgcttcgagc tattgatcaa
     2581 cagaagctca ttcgactgaa ggatttgccg acgcaagctt cctgtcattc cgaagtgacg
     2641 ggtgcaggca gatccgatct ttggaatctt ccgatccggt tcgtcaaagg cgtcgggccg
     2701 aaacgcacca atgtcctgca acgaatgcat atcgagacgg tagaggatgc gctctggacc
     2761 atgccatggc gttacgagga ccgatcggtc atgacaccga tcggaaatct tgtcccgggg
     2821 aggattgcct caatttgtgg ggcgatcgga acatgcgagg cgaaacggac gagaactcga
     2881 cggttgagcg tagtggaagt cggcgtcgaa gatcagaccg ggcgattgca ggtggtcttc
     2941 ttcaatcaac cgtatgtgga ggagattctt acggtcggga ctcgcgtgat gatgagcggg
     3001 cgggtgctct caggccgaca aggatggatg gttccgcgga tggatgtgac gcagtatgaa
     3061 atcatcggag aagacaccga atcgacgttg catgtcggtc gaatcgtgcc gatctatcat
     3121 gagaccaaag gatggacctc tcgccagatg cgggtgttgg tgaagaatct gttggcggac
     3181 catggggttg cgctccttga ccatttgcct gtgcctcttc gggcgcggca gaggctaatc
     3241 ccgattcagg aagcgctgca cgatgctcat ttccccaaga tcggcaccga tcttcagctc
     3301 ctggggcgag ggaagacagc ggcgcatcgt cgattagcgt tcgaggaatt actgctgctc
     3361 caattggcct tagcgaccag gcatcgatcg gtatacgaag agccgaaaga acttcggttc
     3421 aacccgcgga caccgctctt gcagcagctc agtcgtctca tgccgtttcg tctcacgacg
     3481 gcgcaggatc gagtcgttcg cgaaatattc cgagacatga tctcgtcacg gcccatgaat
     3541 cgtctggtgc aaggcgatgt ggggtccggg aaaaccgcag tcgctttgca cgcactggtg
     3601 ctggcctgtg gttcaggcta tcaggccgca ctgatggcgc cgaccgagat tctagcagag
     3661 cagcactacc gaaacctttc tcgaacgttg cagaccctag ggcttcatgc gac
//
LOCUS       DATMTI010000260         6944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_434360, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000260 DATMTI010000000
VERSION     DATMTI010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434360"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VN039_04365"
     CDS             <1..594
                     /locus_tag="VN039_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWV45252.1"
                     /translation="QTALRSEIGKIELDKTFEARTTINAQVVNELDKASEPWGVKVLR
                     YEIKNITPPKDVLNAMEKQMRAEREKRALILSSEGERDAAINQAEGEKQQVIKASEAK
                     KQQQINEAEGAAAAILAIAQATADGLRKVAESIKVPGGPEAVQLRVAEQYIAKFGELA
                     KSSNTLVLPASISDVGSMIALAMNAVKQTGTPVPAKS"
     gene            648..1226
                     /locus_tag="VN039_04370"
     CDS             648..1226
                     /locus_tag="VN039_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HWV45253.1"
                     /translation="MQAFVVTAFADSARVKQQFAHDHADRIVQVASLIANAFRNGNKV
                     LLFGNGGSSTDAAHIAAEFVGRYKRERVPLPAIALATDIAAITCIANDYGYEELFARQ
                     VRAHGRPGDIAIGISTSGNSPNVLKGVEAARDCGLTTIAWTGANGGKLAGLVDYPFIV
                     PSTVTSRIQESHITLGHVLCELVEDHLLGKAS"
     gene            1210..2199
                     /locus_tag="VN039_04375"
     CDS             1210..2199
                     /locus_tag="VN039_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45254.1"
                     /translation="MGKRLDRTPSTRPPALIPPINTSDLKTYPLKKRHSKVRLSDLAA
                     PWKRGGSFSTFYSRLPDILAVKTLRAVTHAVVKAHRRRRPVIMGIGAHVIKVGLSPII
                     TNLMERRIITAVAMNGAGIIHDFELALMGHTSEEVDAEIDEGRFGMAEETGRILNEAI
                     ARGAKDGQGLGEAIGRYINQTAHQFPNRAVSILSMGARLGIPVTVHVAVGTDIIHMHP
                     SADGAAIGATSLLDFRRLTAVVAGMEGGVYLNVGSAVILPEVFLKTLSLGRNLGHPIA
                     NITTVNMDFLSHYRPQTNVVRRPAQKGGQGYSLTGHHEIMLPLLAAAVLEELG"
     gene            2196..2888
                     /locus_tag="VN039_04380"
     CDS             2196..2888
                     /locus_tag="VN039_04380"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HWV45255.1"
                     /translation="MTLTTTPGLSPPLSSEGLQARWQHLNAQYFAGSLRPIEIVWSQR
                     LTASVGMFTCREGPKAFSPLAMTNNRREIRLSLPLFEQLAARTPYAEQELLNTLAHEM
                     IHQWQFDVLKRRPDHGLEFLRKMTQINRSGEVAVTTYHSLEKEVIALSKFAWRCRDCG
                     RLYRRHRRTIQPKRHHCGACRGLLQELVSPTQLIEDRPKPYLTHALNSSPRSAGSTGL
                     GRVPEQLMLELK"
     gene            complement(2926..5673)
                     /locus_tag="VN039_04385"
     CDS             complement(2926..5673)
                     /locus_tag="VN039_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45256.1"
                     /translation="MLWLIAAANVLLVASLIFFLIQTSRRARVERIRSETERIRFQEN
                     EQRLRSMLDRRQALQHAVARVLAGASTVEQAVPDLLQAIVVNLGWHVGLFWRVQDDRR
                     TIICTHGWSVDTTGMQDFLRRSGQEARTAGSDLPRHCWTRGEPLWVEDVAGDSMFAHR
                     SIEATGMLHAACVFPIWLRANVYGVMELFSSEPQAEDRDLLRALGTVGRQIGLFIERT
                     EVEATLHEHEARTSLMIDTALDAVMTMDRTGRITEWNAQAEQIFGWSAREAIGRDVAD
                     TIFPLSHRLNYREYVQRLLEPRDAPLSNRLVEMIGLRHDGREFPVEIAMTPLSVEGAV
                     IFSAFIRDITSRKEAEQALKDYAQRLEHINQQLDAALREAKAATEAKSSFLATMSHEI
                     RTPMSGVIGMTDLLLETTLTDEQRDYAETVRTCGDHLLTIINDILDFSKIEAGKLDLE
                     VIEFDLRLAIEESLDLVAERASSKGLNLACLFHADVPRNLLGDPSRLRQIVMNLTANA
                     VKFTQSGDVVVEVTVEAQSKEDATIRVAVTDTGIGISEQARERLFQSFSQADGSTTRK
                     YGGTGLGLSICKRLVEMMGGMIGVMSRVGEGSCFWFTVNLGKQADGSRSADISAALLA
                     GLRILIVDDKAVNRRMLELMTRKWGMQPTMVHGGSEALDVLNGRDGQPRFDLALLDVD
                     MGPTDGIELARVIKARGEESDIKLVLFTSFGRRGDAKRAKEAGLAAYLTKPIRERQLH
                     DCLVAVITPRPVAPGMPAARESPSLITRHTLAETEAKVDLRILLAEDNIINQKVAARL
                     FQRLGYRVDVVANGREAVEAVSRIRYDAVFMDCQMPEMDGFEATNLIRKLEEPRTPRR
                     VPVIAMTANVMQGDRERCLAAGMDDYLSKPISVDALAGALDRVNQEHERSRPDKSQEA
                     A"
     gene            5885..6367
                     /gene="rfaE2"
                     /locus_tag="VN039_04390"
     CDS             5885..6367
                     /gene="rfaE2"
                     /locus_tag="VN039_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HWV45257.1"
                     /translation="MTTKVLPRDQLLSILSSDRAKGKRIVFTNGCFDLIHVGHTRYLQ
                     AARSLGDVLVVGVNSDASVRTLDKAPDRPIVPEAQRAEVLAALGCVDFVVIFDESNPL
                     QLITAVQPDVLVKGGDWGIDRIVGREIVEARGGVVKTVPLVSGLSTTGLLQRIRSTAK
                     "
     gene            6371..>6944
                     /locus_tag="VN039_04395"
     CDS             6371..>6944
                     /locus_tag="VN039_04395"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45258.1"
                     /translation="MSEANTPTPSWLAPLHSALGQEQTRLCLLGAHGSTAACALTLLN
                     ETRQSTPDRARPWVVVTTNDDSAERMFNDLSFFHELMGRPVEDLAWFPEWETLPYEAT
                     APHVGLIAHRMTTLHRLLVNPPTILVTSVAAAMHRVIPRSIFEQAVFRFETAAAFERE
                     PLITNLLRLGYRRVSVVEIPGEFSIRGGIVD"
BASE COUNT         1479 a         2106 c         1901 g         1458 t
ORIGIN      
        1 cagacagcgc tgcgaagcga gatcggcaag atcgagttgg acaagacctt cgaggccaga
       61 accaccatca acgcgcaggt cgtcaacgaa ttagacaagg cgtcggaacc ctggggcgtg
      121 aaagtccttc gatatgagat caagaacatc actccgccga aagatgtgct caacgcaatg
      181 gaaaagcaga tgcgcgcgga acgggagaaa cgcgcactga ttttgtcgtc ggaaggagag
      241 cgcgatgccg cgatcaatca ggccgaaggc gaaaagcaac aagtgattaa ggcgtctgaa
      301 gcaaagaagc agcaacagat caacgaagcg gaaggtgctg cggctgccat actcgccatt
      361 gcgcaggcca ccgctgatgg actccgcaag gtagcagaat ccatcaaagt cccaggaggt
      421 ccagaagccg ttcaacttcg cgtagctgag cagtacattg ccaagttcgg cgaactcgca
      481 aagtcgagca acacactggt gcttcctgcc agtatttctg atgtcggttc aatgatcgcg
      541 ttagcgatga acgccgtgaa gcagaccgga actccagttc ctgcaaagtc atgacgatag
      601 ggctgcgctg tttgacagtc ttatacgcat catcctagaa tcgcgccatg caagcatttg
      661 ttgtgacggc ttttgcggac agtgcccggg tcaaacagca gttcgcacat gatcacgccg
      721 atcgtatcgt acaggttgcc tcgctcatcg ccaatgcgtt ccgaaacggc aataaagttc
      781 tcttgttcgg gaatggcggg agctcgaccg acgcggcgca tatcgcggcg gagttcgtgg
      841 ggcgatacaa acgcgagcga gtacccttgc ctgccatcgc cctggcaacg gacatcgccg
      901 ccattacctg catcgcgaac gactatgggt atgaagaatt gtttgcccgc caggtgcggg
      961 cgcacggccg accaggcgac atcgccatcg gcatcagcac gagtgggaat tctccaaacg
     1021 tgctgaaagg cgtcgaggcg gcccgcgact gtggcctgac cacgattgcc tggaccggcg
     1081 ccaacggcgg aaagctggcg gggttggtcg attatccctt catcgtccct tctacggtca
     1141 cgtctcggat tcaagaaagt cacatcacgc tgggccacgt cctgtgcgaa ctggtagagg
     1201 atcacctcct tgggaaagcg tcttgatcgg actccctcga ctcggcctcc cgcgttgatc
     1261 ccgccgatca atacgtctga ccttaagacg tatccgctga aaaagcgaca cagcaaggtc
     1321 cggctctccg acttggccgc accgtggaaa cgcggggggt ctttctcgac attctattcc
     1381 cgcctccccg acatcttggc ggtcaagacc ttgcgggccg ttacccacgc cgtcgtgaag
     1441 gcacatcgaa ggcgccgccc ggtcatcatg gggatcggcg cccatgtgat caaggtgggg
     1501 ctttctccga tcatcacgaa tttgatggaa cggcgtatca tcacggcagt cgccatgaac
     1561 ggggcgggaa ttattcacga tttcgaattg gccctcatgg gtcacacctc cgaagaagtc
     1621 gatgccgaga tcgacgaggg tcgcttcggc atggcagagg aaactggccg gatcctgaat
     1681 gaagccatcg cgcgtggcgc gaaagacggc caggggttgg gcgaggccat cgggcggtac
     1741 ataaaccaaa cagcgcatca attcccgaat cgcgccgtca gtattttatc catgggtgcg
     1801 cgactcggca ttccggtgac ggtgcatgtc gcggtcggga ccgacattat ccacatgcat
     1861 ccttcggcgg atggggcagc catcggagcc acttctctcc tggacttcag gcgcctcacc
     1921 gccgtcgtcg ccgggatgga aggcggcgtc tatctgaatg tcggctcggc tgtgattctg
     1981 cctgaagtat tcctgaaaac cctgtcgtta ggacgaaacc tgggtcatcc gatcgcgaac
     2041 attactaccg tgaacatgga ttttttgtcc cattatcggc cacaaaccaa cgtcgtacgg
     2101 cgccccgcgc aaaaaggcgg gcaaggctat tccttgaccg gtcaccacga aattatgctg
     2161 ccgttgctgg ctgcggctgt gctggaggaa ttgggatgac actaaccacc actcccggcc
     2221 tttcaccacc cctctcgtcc gaagggctcc aggctcgttg gcagcacctg aacgcacagt
     2281 actttgccgg ttcactcaga ccgatcgaga tcgtctggag tcagcgcttg accgcctcgg
     2341 ttgggatgtt tacttgtcgc gaggggccaa aggcattctc ccctctcgcc atgacgaaca
     2401 atcgtcgcga aattcgcctt tctctcccgc tgtttgaaca gctcgccgca cgaaccccat
     2461 acgcggaaca ggagctactc aacaccttgg cgcatgaaat gatccatcaa tggcagttcg
     2521 atgtgctgaa gcgtcgaccg gatcatggcc tggagtttct gcggaagatg acgcagatca
     2581 atcgatccgg tgaagtcgcc gtgacgacgt accactcgct ggagaaggaa gtgatcgcct
     2641 tatcgaaatt cgcctggaga tgccgagact gtggacgcct ctatcggcgg caccgaagaa
     2701 cgatccaacc aaagcgccac cactgcggtg cctgccgagg cctgttgcaa gagctcgtgt
     2761 cgccgaccca actgatcgag gatcgtccca agccctacct aactcatgcc ttaaattcat
     2821 caccccgatc agccggatcg accgggctcg gcagagtgcc ggagcaattg atgcttgaat
     2881 tgaagtgatg tggcgaggaa aggaatgtct gatgggaaat gcgagctatg cggcttcttg
     2941 gcttttgtcc ggccgagaac gttcgtgctc ctgattgact cggtccaagg ctccggccaa
     3001 cgcgtctacc gaaataggtt ttgagagata gtcgtccatt cccgctgcga gacaccgttc
     3061 acggtctccc tgcatgacgt tggccgtcat ggcgataacc ggcacacgac ggggagttcg
     3121 gggctcctcc agtttccgaa tgaggtttgt cgcttcgaac ccatccatct ccggcatctg
     3181 gcaatccatg aacaccgcat cataacgaat acgagacacc gcttcgaccg cctcacgccc
     3241 gttggccaca acgtcaactc gataccccaa ccgctggaag aggcgagcgg ccactttttg
     3301 attgatgatg ttatcctcgg ccaggagaat acggagatcc accttggcct cggtttccgc
     3361 aagggtgtgc cgtgtgatga gcgatggcga ctccctcgcg gccggcatgc ctggagcaac
     3421 gggacgtggc gtgatgacag cgacgagaca gtcgtgcaac tgtcgctcac gaatcggctt
     3481 ggtcaggtag gccgccaatc ccgcttcctt ggccctcttc gcatctcccc gccgaccgaa
     3541 cgacgtgaac agcacgagtt tgatatcact ttcttcacct cgtgccttga ttacgcgtgc
     3601 caattcgatc ccatccgtcg gacccatatc cacatccaga agcgcaagat cgaacctcgg
     3661 ctgtccgtcc cgaccgttca aaacatccaa cgcctcggag ccgccgtgga ccatggtcgg
     3721 ttgcattccc catttcctcg tcatcaactc caacattctt cgattaacgg ctttatcatc
     3781 cacgatcaag atgcgaagac ctgccaacag ggcggcggag atatcggcac tgcgggagcc
     3841 gtcagcctgt ttacccagat tcaccgtaaa ccagaagcag ctcccctctc caacccggct
     3901 catcactcca atcattcctc ccatcatctc gacaagacgt ttgcaaatcg aaagaccaag
     3961 gcctgtccca ccatatttcc tcgtcgtgga tccatcggcc tggctgaacg actgaaacag
     4021 tcgttcacgg gcttgttcgg aaatcccgat cccggtgtcc gtgaccgcga cgcgaatcgt
     4081 cgcgtcctcc ttcgattgcg cctcaaccgt cacctccacg accacatctc cgctttgggt
     4141 gaacttgacg gcattggccg tcaagttcat cacaatctgc cggaggcgac tcggatcacc
     4201 aagcagattt cgcggcacat cggcgtggaa gagacaggcc agattgagtc ctttggatga
     4261 cgcgcgttcc gccacgaggt ccaaggattc ctcaatggcc agacgaagat cgaactcaat
     4321 cacctccaaa tccagtttcc ctgcttcgat cttggaaaaa tcgagaatgt cgttgatgat
     4381 cgtcaacaag tggtcgccgc aggtccgaac cgtttcagca tagtcacgct gttcatccgt
     4441 cagcgtggtc tccagcaaaa gatccgtcat cccgatcaca ccactcatcg gtgtccggat
     4501 ttcgtggctc atggtcgcca ggaacgatga cttggcctcg gtggcggctt tggcttccct
     4561 caatgcggcg tccagttgct gattgatatg ctccaaccgc tgggcatagt ccttcagcgc
     4621 ttgctccgct tctttccggc tcgtgatgtc tcggataaat gcgctgaaga tgacggcccc
     4681 ctcgacggat aatggcgtca tggcgatttc taccggaaat tcccggccgt catgtctgag
     4741 accgatcatt tcgaccagcc tgtttgagag aggcgcgtcc ctgggttcaa gaagtcgttg
     4801 tacatactcg cgatagttga ggcgatggga cagtggaaag atggtgtcgg cgacatcgcg
     4861 cccgatcgcc tcacgcgcag accagccgaa aatctgttca gcctgggcat tccattcggt
     4921 aattcggcct gtgcgatcca tcgtcatcac ggcgtcgagg gccgtgtcga tcatcaggct
     4981 ggtgcgggct tcgtgctcat gcagcgtcgc ttccacttca gttcgttcga tgaaaaggcc
     5041 gatctgcctt ccgaccgtgc ccaaggctct cagtaaatcg cgatcctcgg cctgaggctc
     5101 gctgctgaag agctccatga cgccatagac gttcgccctc agccaaatcg gaaagacgca
     5161 ggccgcgtgc agcattcccg tcgcttcgat agatctatgc gcaaacatgg agtctcctgc
     5221 cacatcctcc acccacaacg gctcgccgcg ggtccaacag tgtctcggca gatcggaccc
     5281 tgcggtccgg gcctcctgcc cgcttcttct cagaaaatcc tgcatccccg ttgtatcgac
     5341 cgaccagcca tgtgtgcaga taatggttcg ccggtcgtct tgcacccgcc agaacaaacc
     5401 gacatgccaa ccgagattca cgacaatggc ttgaaggagg tccgggaccg cttgctcgac
     5461 ggtggaggcg cctgcgagca ctcttgccac ggcatgctgg agagcttgcc gccgatccaa
     5521 catactccgg agcctctgct cgttttcctg gaatcgaatt cgttcggtct ccgagcggat
     5581 tctttcgacc cgtgctcgtc tggatgtctg tatgaggaaa aatatcaagg aggcaaccag
     5641 cagaacattg gccgctgcaa tgagccagag catactcacg tccataatca tcgttcacgc
     5701 cccagacaca cccagagccc aggccaggtc cattgcgtag atgtctgtta cgagctctat
     5761 cggtccatta cctgatgact tgaggttaat accaggagtg cgctgaaaca ccgtgcactt
     5821 cggggaacag cacggcgctt tttccgtata gccgacagca cggcgtgcac ggtatgatgg
     5881 ggccatgacc acaaaggtgt tgccccgcga tcaactcctc tccatacttt ccagcgaccg
     5941 ggcaaagggg aaacggatcg ttttcacaaa cgggtgtttc gacctcatcc atgtcggcca
     6001 tacgcgctat ctgcaggcgg ccagatccct cggcgatgtc ttggtcgtcg gtgtgaacag
     6061 tgacgcctcg gtccgtacgc tggacaaggc accggaccga ccgattgtgc ctgaagctca
     6121 acgggcagag gtgctggcag ctttaggctg tgtggatttt gtggtcattt tcgacgaatc
     6181 gaaccctctt cagctcatta cggccgttca accggacgtc ctggtcaagg gcggggattg
     6241 ggggatcgat cggatcgtcg gccgcgaaat tgtcgaagct cgtggaggag tggtcaagac
     6301 ggtcccgctt gtttccggtc tctccacgac cggtctcctt caacgcatcc ggtcaaccgc
     6361 gaagtgatga gtgtctgagg ccaacacccc caccccatcc tggctcgctc cgcttcattc
     6421 ggcacttggc caggaacaaa ctcgtctctg cctgttggga gcccatggct cgactgcggc
     6481 ctgcgccctg actctgctga acgagacccg ccaaagcact cccgaccgcg cccgtccatg
     6541 ggttgttgtg acgacgaacg acgactccgc cgaacgaatg ttcaatgact tgtccttctt
     6601 tcatgaactc atgggtcggc cggtcgaaga cctcgcctgg ttcccggaat gggaaacgct
     6661 cccttatgaa gcgacggcac ctcatgtcgg actgattgcg caccggatga cgacgctcca
     6721 tcgtctgttg gtcaacccac ccaccatcct cgtcacctcc gttgccgctg caatgcaccg
     6781 tgtcattccg cgctcgatat tcgagcaggc ggtctttcgc ttcgaaacag cggcagcctt
     6841 cgaacgagaa ccgctcatca ccaacctcct tcgcctggga taccggcgcg tgtccgtcgt
     6901 ggaaattcct ggggagttca gcatccgtgg cggcatcgtg gata
//
LOCUS       DATMTI010000261         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_731401, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000261 DATMTI010000000
VERSION     DATMTI010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731401"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VN039_04400"
     CDS             <1..1765
                     /locus_tag="VN039_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWV45259.1"
                     /translation="ESIASVLDELRTSPKGLSNTEARERLLEYGPNRIQVKGTPLWKK
                     LIEPFANVFMGVLFVAAVISFFHHETLDGIIIIAIMTVSAVIYYAQQLSTNRVLKALK
                     SHDTQQVETNRDGSVVFVDSEQLIPGDIIHLSEGQKIPADARLIEAQAVRTDESLLTG
                     ESTLVSKRPEAVRRATEVYERTNMLFQGSFIAAGEAVAVVVRTGNATEFGQLAALSSN
                     DEHASPVQRKIDKLLTQIITIVVAVGVVAFGLSILRGSDVTESLRFVIALAVSAVPES
                     LPVAISVILVFGMQRMAKKKALVREVSAIETIGVITTIATDKTGTLTKNKLTVQEIWQ
                     PSHVRTNLIKHMALAINNGSGKIHDPLDTAMLDLVHHEKVILPKQAPLISLPFDQQAA
                     MSGNIWHKGEGYELFVKGAPEHILERSSLTSGEHEEATHALHKLTAQGYRVIALADTY
                     LNGEITSFEAIPKNHPFSFVGFVAVADVLRPEAKRAIAAAQAAGVTVRMITGDHQETA
                     FHIGRELGMVAHPDQVFDSRKMNVMSDQELRAVIDDVRIFSRVIPEHKYRILELLKEK
                     NITAMTGDGVNDGPALVGAHV"
     gene            1772..>2509
                     /locus_tag="VN039_04405"
     CDS             1772..>2509
                     /locus_tag="VN039_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HWV45260.1"
                     /translation="MGSGSHIARDAGDIILLDDNFRSIIDAMREGRIILANIRRMLIY
                     LLATNAGEVMVALGALVIGMPVPLVPVQILWVNLVTDTPMVIPIGLEPGEKSTMSLKP
                     KKPNAPILSRFMMGRVVAMALTMATLTLILYKVYSDRYGVEYGRTIAFAALVVMQWAN
                     ALNARSDYQSLITRLKTVNWPFYIGLAIAITMQLLALFGPLQSVLHVSRVSLGDLYVT
                     GLLSFAVPIIVVEIHKFIGRHFYRSPNI"
BASE COUNT          593 a          706 c          666 g          544 t
ORIGIN      
        1 agaatctatc gcgtcagtac tcgatgaact gcgtacatcg ccaaaaggtc tgtcaaacac
       61 cgaggcccgc gagcgccttt tggaatacgg ccccaaccga atacaggtaa aaggtacccc
      121 gctctggaaa aagctgatag agcctttcgc caatgttttc atgggcgtgc tgttcgtcgc
      181 ggcagtgatt agtttttttc atcatgaaac actcgacggt ataatcatca tcgccatcat
      241 gacggtcagt gccgtcattt attacgcgca gcagctttcg accaaccgtg tcctaaaagc
      301 actgaaatca cacgacaccc agcaggtaga gacgaatcgt gacggtagcg tcgtattcgt
      361 agacagtgaa caactgatac ccggcgatat catccatctt tccgagggtc agaaaatacc
      421 agcggacgca cgtctgatcg aagcacaggc cgtccgcacc gacgaatcgc tcctgaccgg
      481 ggaatcgacc ctcgtctcaa aacgacccga agctgttcgt cgtgccacgg aagtatacga
      541 gcggacaaac atgctcttcc agggctcatt catagccgcg ggtgaagcgg tggccgtggt
      601 agtcaggaca ggcaacgcta ccgagttcgg acaactggcc gccttgagca gtaacgatga
      661 gcatgccagc ccagtacaac ggaaaatcga caagctactg acacagatca ttaccattgt
      721 ggttgcggtc ggcgtggtgg cgtttggtct atcgatactc cgcggctcag atgtgactga
      781 aagccttcga ttcgttatcg ccctggcggt ctcggccgtc cccgagagtc tgccggtggc
      841 gatttcggtc attctggtct ttggcatgca gcggatggct aagaagaagg cattggtgcg
      901 cgaagttagt gccatcgaaa ccatcggcgt catcacgacg atcgcgaccg ataagacagg
      961 taccttgacg aagaataagc tgactgtcca ggaaatctgg caaccgtccc atgtccgtac
     1021 aaatctcatc aagcatatgg cactcgctat caacaacggc tcgggcaaaa ttcacgatcc
     1081 gctagacacg gctatgcttg atctcgttca tcacgagaaa gtcatcttac cgaagcaagc
     1141 gccgctcatt agtctgccgt tcgatcaaca ggccgccatg agtggtaata tctggcacaa
     1201 aggcgaggga tacgagctgt tcgtcaaagg tgcgccggag cacatccttg agcgttccag
     1261 cctgacctcc ggagaacacg aggaagcaac ccatgctctg cataaattga ctgcccaagg
     1321 ttaccgggtc atcgcactag ctgatacata tctgaacggt gaaatcacga gtttcgaagc
     1381 gatacccaag aaccacccgt tcagcttcgt gggcttcgtc gcggtggctg acgttctgcg
     1441 acccgaagcc aagcgtgcca tagccgccgc ccaggcagcc ggtgtcaccg ttcggatgat
     1501 aaccggtgac caccaggaaa cggcattcca tatcggccgg gaactcggca tggtggcgca
     1561 tcccgaccag gtttttgaca gccgtaaaat gaatgtcatg agcgaccagg agctgcgtgc
     1621 cgtcattgac gacgtccgca tattctcccg tgtcataccg gagcataaat accgaatcct
     1681 ggaactcttg aaagaaaaga acatcacggc gatgacaggc gatggggtca acgatgggcc
     1741 ggccttggtt ggcgcgcacg tgtgagttgc gatgggcagc ggctcacata ttgcccgtga
     1801 tgccggcgat atcattctgc ttgacgacaa cttcagaagt atcatcgacg ccatgcggga
     1861 aggacggatc attcttgcca atatccgccg tatgctgatc tacctgcttg ccaccaacgc
     1921 cggcgaggtc atggtggcgc tcggcgcatt agtgattggc atgccggtcc cgctcgtgcc
     1981 cgtccagatc ttatgggtaa acctcgtgac cgacactccg atggtcatcc cgataggtct
     2041 tgaaccgggc gagaagtcta ccatgtcact gaagccgaag aagccgaacg ccccgatcct
     2101 gagccgcttc atgatgggac gcgtggtcgc catggccctg acgatggcta ccctgacgct
     2161 catattgtat aaagtctaca gcgaccgcta tggcgtagag tacggccgga cgatcgcttt
     2221 tgccgccttg gttgtcatgc agtgggcgaa cgcattgaac gcccgtagcg actaccaatc
     2281 tctgattacg cgccttaaga cggtcaactg gccattttac atcggtctgg caattgctat
     2341 aaccatgcag cttctggcgc tattcggacc actccagtcc gttctccatg tcagccgcgt
     2401 ctcactgggc gacctatatg tgaccggatt attgtcattc gccgtcccga ttatcgtcgt
     2461 ggaaatacat aagttcatcg gtcgtcattt ctatcgttca ccgaacatc
//
LOCUS       DATMTI010000262         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_477370, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000262 DATMTI010000000
VERSION     DATMTI010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477370"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VN039_04410"
     CDS             complement(<1..216)
                     /locus_tag="VN039_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45261.1"
                     /translation="MAWDYELDVLIDVIRKAGMEALRFLVDGFETIQKPDQSPVTSAD
                     LAVNQILQFRLASAFPQDGWLSEESPDD"
     gene            409..1512
                     /gene="corA"
                     /locus_tag="VN039_04415"
     CDS             409..1512
                     /gene="corA"
                     /locus_tag="VN039_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWV45262.1"
                     /translation="MSRLDLALESAYKHATMKLVQKRSKKTGLSPGTLIHIGQQRTGA
                     VTMTQFNYAGSRCEESVVTDANELRLPSDDIVTWVNVGGVHKVDVLEVFGKYFGLHPL
                     LLEDIANTDQRPKLDDYETYFFLVMKMLMTSERGDIVVEQVSFVLGRNYVLSFQENGA
                     DVFHSVRDRLRGGKGRLRQNGSDYLLYALIDAVVDQYFAVLEMLGERIESLQERVIAD
                     PKPDTLKDIHALKQQLLFVRRAVWPLREAINNLSRSECPLLHEPTKVFFRDVYDHVVQ
                     IVDTIETLREMVSASLDIYLSSVSYRLNTVMRVLTVITTIFMPLTFIAGIYGMNFEYM
                     PELKWPWGYPMALGLMGIVAAVMLIGFRRKKWL"
BASE COUNT          345 a          405 c          430 g          366 t
ORIGIN      
        1 atcatcaggc gattcctctg agagccatcc gtcctgagga aatgccgacg ccaggcgaaa
       61 ttggagaatc tgattcactg ccaggtccgc cgaggtcacc ggcgattggt ccggcttctg
      121 aatggtctca aatccatcga ctaggaaccg taacgcttcc atcccggcct ttcggatcac
      181 atcgatgaga acatccagtt catagtccca tgccatcacg ggagagtaac aggcttggag
      241 acagatgaaa agaaactaac aggctgttga aaaagtccgc cagcggcgtt ctcgcggcgc
      301 ttagaggctc aacgtacgac tcaaagtacg cttcgcctcc tcgcttgctg cggccttgca
      361 ggacggcctt tttgaacagc ctgtggtctt gtgagatcgt ctgctagcat gtcccggctt
      421 gacctggctc tggaatctgc gtacaagcac gctacgatga aactggtgca gaaacggtcg
      481 aagaagacgg gactctcgcc cgggacgctc attcacatcg gtcaacagcg aaccggggct
      541 gtgaccatga cccagttcaa ttatgccggt tctcgatgcg aagagtctgt cgtcacagac
      601 gcgaatgagc ttcgactgcc ttccgatgac atcgtcacgt gggtgaatgt cggtggagtc
      661 cataaggtgg atgtcctgga ggtcttcgga aaatatttcg gcctccaccc ccttcttctg
      721 gaagacatcg ccaatacgga ccagcgtccg aagctcgatg actatgagac ctatttcttt
      781 ctcgtcatga agatgcttat gacctctgaa cgaggggaca ttgtggtcga acaggtgagt
      841 ttcgtccttg gccgcaatta tgtcctttcg ttccaagaaa atggggcgga cgtctttcac
      901 tcggtgcggg atcgtctgcg aggcggcaag gggcgccttc ggcaaaatgg atcggactat
      961 ctcttgtatg cgctcattga tgccgtggtc gaccaatatt tcgctgtgct cgaaatgctc
     1021 ggcgaacgga tcgaatcgct gcaagaacga gtgatagccg atccgaaacc ggatacactc
     1081 aaagacattc atgcgctcaa acagcagttg ttgttcgtgc ggcgcgccgt ttggccactg
     1141 cgggaagcga tcaacaacct gtctcgttcg gaatgtccct tgttacacga acccaccaaa
     1201 gtgttttttc gggacgtcta cgaccatgtg gtgcagatcg tcgatacgat cgagacattg
     1261 cgcgaaatgg tctcggcgag cctcgacatc tatctgtcaa gcgtcagtta tcggctgaac
     1321 accgtcatgc gggtgctgac ggtgatcacg acgattttca tgccgctcac gtttatcgcg
     1381 ggcatctacg gcatgaattt cgagtatatg cctgagttga aatggccgtg gggctacccg
     1441 atggcgttgg gcctaatggg aatcgtggcg gccgttatgc tgattgggtt ccgtcgaaag
     1501 aaatggctgt agcaggatgt tgaaaaagcc ctccagcttc gttctc
//
LOCUS       DATMTI010000263         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_604276, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000263 DATMTI010000000
VERSION     DATMTI010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604276"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1590)
                     /locus_tag="VN039_04420"
     CDS             complement(<1..>1590)
                     /locus_tag="VN039_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWV45263.1"
                     /translation="LHAAHGINHFFVLAPNLTIYNKLITDFTPNTPKYVFTGIAEFAA
                     EPPTIITGDHYESGVAARLTSLPGFGQEVHINIFNISKISSEVRGGKAPRIKRLSEYI
                     GQSYFEYLAGLEDLVLLMDESHRYRASAGVRAINELKPVLGLELTATPFVESSKGPVR
                     FKNVIYDYPLTQAMADGFVKEPAVVTQKNFNPSQFNAAQLENIKLEDGIRLHENTKVE
                     LETYARQAGQRVVKPFMLIIARDTTHAGLLMQQFQSLFEGRYKDKVIQVDSSKTGAEE
                     DEMVQRLLAVESPEEPTEIVIHVNMLKEGWDVTNLYTIVPLRAANARTLIEQSIGRGL
                     RLPYGRRTGVSTVDRLNIVAHDRFQEILDEANKPDSVIRLMKVELDPVTDLQKTKTVV
                     AQSKIEEQLHGASVSGPLGGSAQAAPIFAGEVERGIAQAAYKVIQKYEHLPSSADLLK
                     PEIQTKIVEEVKQSIIPSQQALPGMAPAPDIASVVEKTAQLVVQQSIDIPRILVVPKG
                     EVTTGFHLFSLDCSNINYQPV"
BASE COUNT          340 a          432 c          423 g          395 t
ORIGIN      
        1 tcaaccggct gataattgat gtttgaacaa tccagcgaaa agagatgaaa gccggtcgta
       61 acctccccct tcggcacgac tagaatgcgc ggaatatcga tcgattgttg cacgaccagc
      121 tgagcggtct tctcgactac cgatgcaatg tccggtgcag gagccatgcc cggcaaggcc
      181 tgctggcttg ggatgataga ttgcttcacc tcttctacaa tcttggtctg gatctccggc
      241 ttcagaaggt cagcagagga aggaagatgt tcatactttt ggatgacctt gtacgctgct
      301 tgcgcaattc ctcgttctac ctcaccggca aaaatgggag ccgcttgcgc agaccctcct
      361 agaggaccag acacagaggc cccgtgcaat tgctcctcga tctttgactg agccacgacg
      421 gttttcgtct tctggagatc ggttaccgga tcgagttcca ccttcatcaa tcggatcacg
      481 gaatccggct tattggcctc atcgaggatt tcctgaaacc ggtcatgggc cacaatgttc
      541 agccggtcga ccgtactgac gccggtccgt cgtccatagg gaaggcggag gcctcggcca
      601 atcgattgtt cgataagcgt tctcgcattc gcggccctga gcgggacgat tgtatagaga
      661 ttcgtcacgt cccagccttc tttgagcatg ttgacgtgga tcacgatttc ggtcggttcc
      721 tcgggacttt ccacagccaa caggcgctga accatctcgt cctcttccgc accggtcttg
      781 ctggagtcca cttgaatgac cttgtctttg taccgaccct cgaaaagcga ttgaaactgt
      841 tgcatcagta ggccggcatg ggtggtgtct ctggcaatga tcagcatgaa aggctttact
      901 acccgctgtc ctgcctgcct cgcgtaggtt tccagctcga ccttcgtatt ctcgtggaga
      961 cgtatgccat cctccaactt gatattttcg agttgtgcgg cgttgaactg gctcgggttg
     1021 aaattcttct gcgtcaccac agccggctct ttgacaaagc cgtcggccat tgcctgggtc
     1081 agaggatagt catagatgac attcttgaac cgcaccggcc ctttcgatga ttccacaaaa
     1141 ggagtcgccg tgagttccaa cccgaggacc ggcttcaatt cattgatggc ccgaaccccg
     1201 gccgacgcgc gatagcgatg ggactcgtcc atgagtaaga cgagatcttc aaggcccgcc
     1261 aaatactcga agtaactctg tccgatgtac tcggatagac gcttgattcg cggcgctttt
     1321 cctccgcgaa cttccgagct aatctttgag atattgaaga tgttgatgtg cacttcttgg
     1381 ccgaacccag gcagagaagt cagtcgagcc gccactccgg attcataatg gtcaccggta
     1441 atgatcgtcg gcggttccgc cgcaaattcg gcaatcccgg tgaagacgta ttttggtgtg
     1501 ttgggcgtaa aatctgtgat gagtttgttg tagatggtca gattcggcgc cagcacgaaa
     1561 aagtgattaa tcccatgcgc ggcatgcagg
//
LOCUS       DATMTI010000264         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_10360, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000264 DATMTI010000000
VERSION     DATMTI010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10360"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..949)
                     /locus_tag="VN039_04425"
     CDS             complement(170..949)
                     /locus_tag="VN039_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HWV45264.1"
                     /translation="MKVLCIGDIMGEPGRRAVARAVPRLIAQHRIDAVIANGENLAGG
                     FGVTPELAEELFETGISVITTGNHAWDKKEAVDYFSREPRLLRPANYPAGVPGNGSVV
                     IETAGGERLAVLQLMGRVYMPTTDCPFQTAKRELSRLKRETAAIVVDMHAEATSEKMA
                     MGHFLDGEVIAVVGTHTHVQTADEQILPKGTAYITDIGMTGPLHSVIGVKKEVAIEKF
                     LTAMPRRFEVASGPSVFCAVLLELDPRLGKAIAFERIRLID"
     gene            complement(946..>2250)
                     /gene="rny"
                     /locus_tag="VN039_04430"
     CDS             complement(946..>2250)
                     /gene="rny"
                     /locus_tag="VN039_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HWV45265.1"
                     /translation="MEKRLIQREGGLDGKLVALEKRENESRKHEADFAKREEGLAAKE
                     SACAKAEREHRDALERVAGMTADEAKKQLILEMESQAKLDAVGIAKRTIEEARENAER
                     EAREIITTSIQRVVRDYVSESTISVVPIPNDAMKGRIIGREGRNIRALEAATGIDLII
                     DETPEAVIISGFDPLRREIAKVSLERLMHDGRIHPTRIEEIVEKVKVDIDKLMYEEAE
                     KIIFELGLSDFHPELIKVLGRLKYRTSYGQNNLYHAREASYICGIMASELGLDVRLAR
                     RGALLHDIGKAVSHEEEGPHAMLGAEIAKKYGESPKIVNAIAGHHEQVEPICPESVLV
                     AAAEALSAARPGARREALESYVKRLEKLESLATGHKGVQKAYAIQAGREIRVIVRQED
                     ITDAESFQLSRELAKKIEQELTYPGQIKVTVIRESRYVEYAK"
BASE COUNT          438 a          724 c          553 g          535 t
ORIGIN      
        1 gtcacgtctg cggacgggcg cgagctggtg aacgcccggc gtcttaaagg ggcgaggcgt
       61 atcctttttc acccacccgc cccgagctgc tcggacagct cttgcccggt ggtacgttga
      121 ggccttgagc gatgcgagaa cgctgctggc ggactttttc aacagcctgc taatcgatga
      181 ggcggattcg ttcaaacgcg atcgccttgc ctaaacgagg atcgagctca agcagaaccg
      241 cgcaaaatac ggacggtcct gaggcgactt caaaccgacg cggcatcgcg gtcaaaaact
      301 tttcgatcgc cacttctttc ttgactccga tcacagaatg gagcggcccc gtcatgccga
      361 tatccgtgat gtacgcagtg cccttcggaa gaatttgctc atcggcagtc tgcacatgcg
      421 tatgcgtacc gacgacagcg atcacttctc catccaagaa atgccccatg gccatctttt
      481 cagatgtggc ttccgcatgc atgtcgacca cgatcgcggc agtctcccgc ttcaaccgcg
      541 acaactcgcg ttttgccgtc tgaaacgggc aatcggtcgt cggcatgtac acccgaccca
      601 tcagctgtag gactgccagt cgctcgcccc ctgcggtctc aatgacgaca ctcccgtttc
      661 ccgggacccc cgccggataa tttgccggac gcaacaagcg gggctcccga gaaaaataat
      721 cgactgcttc tttcttatcc caggcatggt ttccggtcgt gatgaccgat atgcccgttt
      781 caaacagttc ttcggccagc tccggcgtga ccccgaagcc tccggccaga ttttcaccgt
      841 tggcgatgac cgcatcgata cggtgctgtg cgatcagccg aggcaccgcc cgagcgactg
      901 cccggcggcc cggctctccc atgatatccc cgatacataa gaccttcatt tggcgtattc
      961 cacgtatcgg ctttctcgaa tgacagtgac cttgatctgt cccggatacg tcaactcctg
     1021 ttcgatcttc ttggcaagtt cacgagaaag ttggaacgac tcggcatcgg taatgtcctc
     1081 ttgcctcaca atgacgcgaa tttcacgccc ggcttggatc gcgtatgctt tctgcactcc
     1141 cttatgcccg gttgccagcg attccagctt ttccaaccgc ttcacatagg actccagagc
     1201 ctcccgtcgc gcaccaggcc gtgccgccga caacgcctcg gcagccgcca ccaggacact
     1261 ctccggacag atcggttcca cctgctcgtg atgcccggcg atcgcattga caatcttggg
     1321 agattcgccg tattttttgg cgatttccgc ccccagcatg gcatgcggcc cctcttcttc
     1381 atggctgacc gccttgccga tgtcatggag caaggctcct cgccgagcca gtctgacatc
     1441 gagacccaat tccgacgcca tgatgccgca gatatacgat gcttcgcggg catgatagag
     1501 gttattttgc ccgtagctcg ttcgatactt gagacgcccc agcactttga tcaactcagg
     1561 atgaaaatct gagagcccca gctcaaaaat aattttctcc gcctcttcat acatcagctt
     1621 gtcgatgtcg accttgacct tttcgacaat ctcctcgatg cgcgtgggat gaatgcgccc
     1681 atcgtgcatc agccgttcta atgacacttt ggcaatctcg cgccgcaacg gatcaaatcc
     1741 ggagataatc accgcttcag gggtttcatc gatgatcaga tctatgcccg tcgccgcttc
     1801 aagcgcgcgg atattccgcc cttcccggcc gatgattcgg cccttcatgg catcgtttgg
     1861 aatgggaact accgaaatcg tggattccga cacataatcc cgaacgacgc gttgaattga
     1921 ggtggtaata atctcccgag cttcccgctc cgcattttca cgcgcctctt cgatcgttcg
     1981 cttcgcaatc ccgacggcat cgagtttcgc ctgcgattcc atttcgagaa tcaattgttt
     2041 cttggcttca tccgccgtca taccggcaac ccgttccaac gcgtcgcggt gttcgcgttc
     2101 ggccttggca caggcagact ccttggctgc gagcccctct tctcgcttcg caaaatccgc
     2161 ctcgtgcttg cgtgactcat tttcccgttt ttccaaggcg acgagctttc cgtccaaccc
     2221 tccttctcgt tggataaggc gcttctccat
//
LOCUS       DATMTI010000265         3899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_519699, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000265 DATMTI010000000
VERSION     DATMTI010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519699"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VN039_04435"
     CDS             complement(<1..1188)
                     /locus_tag="VN039_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HWV45266.1"
                     /translation="MLIDTSLLSETEQRFADRQTKREEGVRLIRAGNLLQADSPERVQ
                     ARLNRLGIDRRVAERVAGTKTDLRKETTRRIVDGAEQAKAPGNTEQEKVEAVMAETLG
                     IEPGAMRPAEMTVNELLLERIVGRNDLLGIAYMDRGLAASRTVGCVRLFDNGRCVGSG
                     TGFMVSPQLLMTNNHILEDTAMGRQARVVFNFQDDPEGNALPPSTFLLDPDTFFITSQ
                     SLDFTLIAVNRQAAEGTVDLEEIGWSRLIEAEGKVINGEFVTIVQHPNGQPKQIALRE
                     NQIIDILENFLHYHTDTAPGSSGSPLFNDQWEVVGLHHSGVPRRDSQGRILTRDDTLW
                     TSAMGDAAIHWIANEGVRISRILRHVKSQSLGGEARRLREQIFEEKPPLTRTKPLSIS
                     VVAS"
     gene            complement(1368..2384)
                     /locus_tag="VN039_04440"
     CDS             complement(1368..2384)
                     /locus_tag="VN039_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWV45267.1"
                     /translation="MDVLSEVLKAVKLDGAVFFNGEFSSPWCAREPDAGTLASYLPTR
                     SKHVIIFHLITEGRGYVRIEENGRAVPLEAGDIVILAQGDAHLMGNGPFVTPIDSAIQ
                     LQQVLAEGRMISHCGGGGELTKLICGYLTCDPQLSQVFLVGLPPIVKVHIRDSPSGQW
                     LEDTLRYSIDHAEVSGPGGAAVIAKLSEVLFVETLRRYIAKLPPSHTGWLAGVRDPDV
                     GKALALLHKQPAQPWTIASLANEVGVSRSVLAERFRHYLSDTPIGYLTRWRLQLAAQV
                     LCSTAKSVAEVANDVGYESEPSFNRAFKRQFGVPPARFRSHVKPTHASSSAPTQQTDR
                     TAHR"
     gene            2580..3398
                     /locus_tag="VN039_04445"
     CDS             2580..3398
                     /locus_tag="VN039_04445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV45268.1"
                     /translation="MTSAAIGEIDSVKARLKSVWTAGDYDRFSRYMEGGAREFYERLN
                     IAPGSRLLDLACGSGQLALMAAKDGIDVTGVDIAANLVERAQVRAQAEGVRVRFEEAD
                     AEALPFDDASFDVVTSLIGAMFAPRPQLVVQELLRVCVPGGIIAMANWTSQGFIGRMF
                     KAVSKFIAPSGMPSPVLWGDEAIVRERLGHGLSELSLTRRQYLFSYPFSPSEVVEFFR
                     LYYGPTNRAFASLDADGQTSLRRELEALWTAHNQSGSDCTTVYGEYLEVIGIRV"
     gene            3505..>3899
                     /locus_tag="VN039_04450"
     CDS             3505..>3899
                     /locus_tag="VN039_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45269.1"
                     /translation="MKPLGSLALTERGNTMMSAHIIGFTMAVFLTGAALSSGTESTAT
                     PAPPSLSDTVPLYTNLGSHHKRITTRVAATQQYFDQGLRLVYGFNHAEAIRSFTRAAE
                     LDPTCAMCYWGIALAHGPHVNAPMDSTSG"
BASE COUNT          806 a         1101 c         1129 g          863 t
ORIGIN      
        1 cgacgcgacg acggatattg ataacggctt tgtacgggtt aggggtggct tctcttcaaa
       61 gatctgctct ctcaaccggc gtgcttcccc gccgagggat tgactcttga catgtcttag
      121 aatccgactg atccgtacac cttcgttggc aatccagtgg atcgccgcat ctcccatggc
      181 ggacgtccag agggtatcgt cgcgcgtgag aatccttccc tgtgaatccc gcctgggaac
      241 tccggaatga tgcagtccca ccacttccca ttgatcgttg aacagaggtg atcccgatga
      301 accgggcgcc gtatcggtgt gatagtggag aaaattttcc aagatgtcga tgatctgatt
      361 ctcgcgcaag gcgatctgtt taggttggcc gttcgggtgc tgaacgatcg tgacaaattc
      421 accattgatc accttgcctt cggcctcgat caagcgagac catccgatct cctcgagatc
      481 gaccgttcct tccgccgcct gtcgattcac ggcaatgagg gtgaaatcca atgattggct
      541 cgtgatgaag aacgtgtcgg ggtcgagtaa aaatgtacta ggcggcaagg cgttgccctc
      601 ggggtcgtcc tgaaaattga agacgacgcg cgcttgccgc cccatggctg tatcttcgag
      661 gatatgattg ttcgtcatca gcaattgtgg cgacaccatg aatccggtac cggatcccac
      721 gcaccgcccg ttgtcgaaga gtcgtacgca tccgactgtg cgacttgcgg cgaggccgcg
      781 atccatatag gcaatgccca acaaatcgtt ccgacctacg atccgctcca agaggagttc
      841 attcaccgtc atctccgcag ggcgcatggc ccctggctcg attcccagtg tctcggccat
      901 cactgcttcc accttctcct gttccgtatt gccgggggcc ttggcctgct cggcgccatc
      961 cacgattcgt cgtgttgttt ccttccggag atcggtcttt gtgccggcca cccgctccgc
     1021 cactcgtcga tcaatgccca aacggttcaa acgcgcttgt acccgctccg gcgagtcggc
     1081 ctgcagaaga ttccctgcac gaatgagtcg gacaccttcc tcacgcttgg tttgtcgatc
     1141 tgcgaatcgc tgttcggtct cgctcagtaa ggatgtgtca atcagcatcg ctctccttcg
     1201 gcacaggaca tgtgccttgc agtcgttggg cggtatcgac agcctgtgtg ttgaccggcc
     1261 ggtctatcta agtacttttg tggcggaaga atcaaggctt catggtatct tgtgaaccat
     1321 aatagcccca atgtcattaa gtctgcgctc gggcggtgtc gagctgccta ccgatgagcc
     1381 gtccgatccg tttgttgagt gggagcggaa gaggaggcgt gagtcggctt cacgtggctg
     1441 cggaaccgtg ccgggggtac cccgaactgc cgcttgaagg cgcgattgaa ggaaggctcc
     1501 gactcatatc ccacgtcgtt tgccacttcg gccacactct tggcggttga acacaatact
     1561 tgagcggcga gctgcagcct ccagcgcgtc aggtacccga tcggcgtgtc tgaaagataa
     1621 tgccgaaatc gttcggccaa cacggaacga gatacaccga cctcattcgc caacgaggcg
     1681 atcgtccagg gttgagccgg ctgtttgtgc aggagggcca gcgcctttcc cacgtccgga
     1741 tcacgtacgc cggccaacca acctgtatgt gacggcggca acttcgcgat gtaccgccgc
     1801 aacgtttcca cgaacaacac ctccgacaat ttggcgatga cggcagcgcc gcctgggccg
     1861 gaaacctcag cgtgatcgat agaatatcga agggtgtcct ccaaccactg tcctgacggg
     1921 ctgtcacgga tatggacctt gacgatcggg gggaggccga ccaggaagac ctggctgagt
     1981 tgcggatcgc aagtcaggta gccgcagata agcttggtga gttctcctcc gcctccacaa
     2041 tgagagatca tacgtccttc ggccaacacc tgttggagtt gtatcgcgct gtcgatcggt
     2101 gtgacgaaag ggccgttgcc cataaggtgc gcatcgcctt gcgcaaggat gacgatatcg
     2161 ccggcttcaa ggggtaccgc ccggccattt tcttcgatgc gaacgtagcc tcgtccttcg
     2221 gtgatcaggt gaaagataat gacgtgcttc gaacgggtgg ggagatacga tgccaacgtg
     2281 cctgcatcgg gctcgcgtgc gcaccacggg gatgagaatt caccgttgaa gaacactgcg
     2341 ccgtccagtt ttacggcttt caggacttca gagagcacat ccatatcgat ggctaccagt
     2401 aagtggtcct gctaagcaag tcggacgcct ggataaacga gccgcagctt agcatgctct
     2461 tgctaagaga gccagactcg ctgataagac gccgtcacgc ctgtccgata tagtgcgaac
     2521 agagacagcg cggcaggttc aacagcgccg ttcatcaaca atagaaggag gaagtcatca
     2581 tgacgagtgc agcgattggg gaaatagaca gtgtcaaggc tcggctcaaa tcggtctgga
     2641 ccgccggaga ctacgatcga ttctcacgat atatggaggg cggcgcacga gaattttacg
     2701 agcggctcaa catcgcgccg gggtcacggt tgctggacct cgcttgcggg tccggtcaac
     2761 tggctctgat ggcggcaaaa gacggtattg atgtaaccgg cgtggatata gcggccaatt
     2821 tagtggagcg ggcacaggtc cgcgcacaag cggagggagt gagggttcgt ttcgaggagg
     2881 ccgatgccga ggcgcttccg ttcgacgatg cgagcttcga cgtcgtgacg agtctgatcg
     2941 gggcaatgtt cgcgccccgg cctcagctgg ttgtccaaga attgcttcgt gtgtgcgtgc
     3001 cgggcggcat cattgccatg gccaattgga cgtcgcaggg attcattggt cggatgttca
     3061 aggccgtatc aaagttcatc gcgccgtccg ggatgccgtc tccggttttg tggggagacg
     3121 aagccatcgt ccgtgagcga ctcggtcatg ggctctcgga actcagtctc acgaggcggc
     3181 agtatctttt cagctatcct ttttcaccat cggaggtggt ggaattcttt cgtctctact
     3241 atggaccgac aaatcgcgcg ttcgcctcgc tcgatgccga cggccaaacg tcattgcggc
     3301 gggaattgga agcactctgg acggcgcaca atcaatccgg gtcggattgc acgacggtgt
     3361 atggtgaata tctggaagtc atcggtattc gggtgtgacg tgggatttca agaagcaacg
     3421 acgagatctc actctatttg ttcgtacagt agtacgacgt aaaaacggat acaatatgtt
     3481 cctatcatgc agaaggaggt tgtcatgaaa ccattgggat cactcgcatt gactgaacgg
     3541 gggaacacca tgatgtccgc ccacatcatc ggattcacca tggctgtttt tctgaccggt
     3601 gctgctctca gctcgggcac agagtcgacg gcaacgcccg cgccgccgag cctttctgac
     3661 acggttcctc tctatacgaa cctcggctct catcacaaac gcatcaccac gcgagtcgcg
     3721 gccacgcagc agtacttcga ccagggccta cgactcgtct acggctttaa ccatgcggaa
     3781 gcgatccgct cgttcacgcg cgccgcagag ctcgatccca cctgtgccat gtgctactgg
     3841 gggatcgcac ttgcccacgg gccgcacgtc aacgctccta tggactcgac gagcggaat
//
LOCUS       DATMTI010000266         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_816942, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000266 DATMTI010000000
VERSION     DATMTI010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816942"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VN039_04455"
     CDS             <1..624
                     /locus_tag="VN039_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWV45270.1"
                     /translation="QEGYATIDELAAAVGFGHLATAQLIGRLMAPASAGARTQSEPVS
                     APKAVGTRRAEPGVQVKGGRDLLMQLSRCCNPVPGDKILGYITRGRGLTIHSIDCPNL
                     EALDYDRERLVEVEWDTATPSQHAVKIAVIAEDKTGVLANVSSAIAECKANISRAEII
                     TREDRKAELDFVVEIADTSHLNRVLKTIERVGGVITARRVRSWQERS"
     gene            complement(907..1266)
                     /locus_tag="VN039_04460"
     CDS             complement(907..1266)
                     /locus_tag="VN039_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWV45271.1"
                     /translation="MATMRTMPFGSSTLFFLCSMGLFGSLLFGMPASFASDGGLVHIQ
                     TPSGVTIHAEIADTPLKRAVGLMYRDHLKKDHGMLFFFSQPQAWSFWMKNTKIALDLI
                     WLDAKKRVTHIERNVPI"
     gene            1389..1574
                     /locus_tag="VN039_04465"
     CDS             1389..1574
                     /locus_tag="VN039_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45272.1"
                     /translation="MQEKRRVLYKKGVFVCPGCRQSYVQEKWIEEWRIRCHRCTYRGT
                     LTEVSVEEHMEEETFRR"
BASE COUNT          392 a          409 c          473 g          372 t
ORIGIN      
        1 caggaaggct atgcgacgat tgatgaactt gctgcggcgg tgggatttgg tcaccttgca
       61 acggctcaac tcattggacg gctgatggct ccagcttcag ctggtgctcg gactcagtcc
      121 gaacctgtca gtgcccccaa agccgtcggc acgagaagag cggagccggg tgtccaagtg
      181 aagggaggac gcgatctgtt gatgcaactg tcgcgatgct gtaatccggt tcccggcgac
      241 aagatcttgg gatacatcac gcgcggaagg ggcctcacga tccattcgat cgattgtccc
      301 aacctggaag cgttggatta tgatcgagaa cgtttggtgg aagtggagtg ggatacggct
      361 acgccgagtc agcatgcggt aaaaatagcc gtcatcgcgg aggataagac gggagtgttg
      421 gcgaatgttt cctcggctat cgccgaatgc aaagcgaata tcagccgtgc cgaaatcatc
      481 actcgggaag accgtaaggc ggagcttgat tttgtcgtgg aaatcgctga tacgagtcac
      541 ctgaatcgcg tactgaaaac cattgaacgg gtaggcggtg tcattacggc acggcgggta
      601 cggtcctggc aggaacgatc atgacaggct gctgaaaaag tccgccagct gcgttctcac
      661 ttcgctcaga ggctcaacgt accgaagcgt acgcctcgcc ccttcgctcg ctgcagcctt
      721 gctggacggc ctttttgagc agcctgctaa ggagtgtggc aagagaagat gctagggctg
      781 cccgaataac aattttgtcc ctttctcgat cttgtacccg tcaatcgtac ctccggcaat
      841 ttcaagcacg tacatcgcat cgtcattgtt tgggcggtat tggggacagg agtcgtcggt
      901 cttcgttcaa atcggaacgt tgcgttcaat atgggtgacc cgctttttag cgtccaacca
      961 aatcagatcg agcgcgattt tggtgttttt catccagaac gaccaggctt gtggttggct
     1021 gaaaaaaaat aacatcccat gatctttctt gaggtgatcc cgatacatca agccgaccgc
     1081 gcgcttcagc ggcgtgtcgg cgatctcggc atggatcgtg acgcccgagg gggtctgaat
     1141 atgtacgagc cctccgtcag aggcgaagga tgccggcata ccgaagagaa gtgatccaaa
     1201 gagccccatg gaacacagaa agaagagagt tgaactcccg aaaggcatgg tgcgcatcgt
     1261 agccatccgg cctagctcag tcaaggaacg tattgcagcc attgcgatca gatagcgttc
     1321 cactccgtca atcttgcaat cctttcgggc aatgtgccat cctcagacgc ggttaggaag
     1381 aggagatcat gcaagaaaaa cgacgggtgt tatataaaaa aggtgtgttt gtctgcccag
     1441 gatgtcgcca gtcctacgtc caagagaaat ggattgaaga atggcggatt cggtgccacc
     1501 gctgtaccta ccgtggaacg ctgacagaag tgtcggttga ggaacatatg gaggaagaga
     1561 cgtttcggcg ctaatccctt ccattttgtt ctatccggat cgtaacattg tgtcaccata
     1621 cttcaccaag actcatgtct ctcgtg
//
LOCUS       DATMTI010000267         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_604368, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000267 DATMTI010000000
VERSION     DATMTI010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604368"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VN039_04470"
     CDS             complement(<1..249)
                     /locus_tag="VN039_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45273.1"
                     /translation="MANQLNVQIIEQGVRNAIVKITGILDTSNQASTVVVDPANFSGN
                     APDNQSNWQHPDHFRIDYIDYSIQDQLEVTLWWAGTPDL"
     gene            complement(322..1236)
                     /locus_tag="VN039_04475"
     CDS             complement(322..1236)
                     /locus_tag="VN039_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45274.1"
                     /translation="MPVITTGSHPKLLWPGVHATWGQVYEGYEKEHEDLYEMLDSEMA
                     YEQDVQVTGFPLAVVKGQGAPVVFAGENQGWVTTYNHIAYGLGYAVTKEELDDNQYEV
                     VAMRRAKANAFSMAQTVETVAAFLYNNAFTSSFYAMPDGQALISASHVNVTGGTSSNI
                     LSPSADLSEVALEDICIQIMGAQNDNGLIINLMPQSLHVDRSEFFNANRILKSVLQSG
                     TTSNNINVLRAVNAFPKGIKLNHFFNTAGNWFVRTNCPNGMQFFWRTRPEFDQDNDFP
                     TKNALAASYMRFSVGATDFRGIYGSPHA"
     gene            complement(1251..>1546)
                     /locus_tag="VN039_04480"
     CDS             complement(1251..>1546)
                     /locus_tag="VN039_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45275.1"
                     /translation="VEDSPDVLYSIMEPTQTTQFTAADIGLNSKLAAGSTSTFQSDWT
                     LAAGDGSTTPETTSTLPVQLLGLVQTPGNSFGAGAQWLCRINVHQFAAGVAGV"
BASE COUNT          326 a          386 c          492 g          342 t
ORIGIN      
        1 taggtcgggg gtaccggccc accacagcgt aacctcgagc tggtcctgga tggagtagtc
       61 gatgtagtca atccggaagt ggtcggggtg ttgccagttg ctttgattat cgggtgcatt
      121 tcccgaaaag ttggccggat caacaaccac tgtagaagct tgattgctgg tgtccaagat
      181 ccctgtgatc ttcacgatcg cattacggac accttgttct atgatttgga cattcagttg
      241 gtttgccatg ctagttcctc gacggcgaga gtgtgggggt aaactacgtt gaaggggcct
      301 gtcacacccc ttgggcctag cttacgcgtg cggcgaaccg tagataccgc ggaagtccgt
      361 agcacccaca gaaaagcgca tgtaagaagc ggccagagcg ttcttggtcg ggaagtcgtt
      421 gtcctggtcg aattcaggac gggtgcgcca gaagaattgc atgccgttgg ggcagttcgt
      481 gcggacaaac cagttgcctg ccgtgttgaa gaagtggttc agcttgatgc ctttggggaa
      541 ggcattgaca gcacgcagaa cgttgatgtt gttcgacgta gtgccagact gaagcacaga
      601 cttcaagatg cggttggcgt taaagaactc ggagcgatcg acgtgaagcg actgcggcat
      661 gaggttgatg atcaggccgt tgtcgttctg cgcacccatg atctggatgc agatgtcttc
      721 gagagcaact tccgaaaggt cagccgaggg ggacaagatg ttggacgacg tgccaccggt
      781 gacgttgacg tgggaagccg agatgagggc ttggccgtcc ggcatggcgt agaacgagga
      841 ggtgaaggcg ttgttgtaca ggaaggccgc cacggtctcg acagtttggg ccatcgaaaa
      901 cgcatttgcc tttgcacgac gcattgcgac cacttcgtac tggttgtcgt ccagttcttc
      961 tttggtcacc gcatagccca gaccgtaggc gatgtggtta taggtggtca cccagccttg
     1021 gttctcacca gcaaagacga ccggggcccc ttgacctttc acgacagcca gcgggaagcc
     1081 ggtgacttgg acatcttgtt cgtaagccat ttcggaatcg agcatctcgt agaggtcctc
     1141 gtgttccttt tcgtagcctt cgtagacttg gccccacgtg gcatgaacgc caggccacag
     1201 cagtttggga tgcgagccgg tagtgatgac gggcatgtta gcctcctagg ttaaacgccg
     1261 gcaacgccag ctgcgaattg gtgaacattg atgcggcaga gccattgggc ccccgcaccg
     1321 aaggaattgc ccggagtttg aaccagaccc agcagctgga cgggcagggt ggaggtcgtt
     1381 tccggggtgg tggaaccgtc accggcagcc agggtccagt ccgactggaa ggtggacgtg
     1441 gagcccgcag ccaacttact gttcagacca atgtcggcag ccgtgaactg agtcgtttgc
     1501 gtgggctcca tgatcgagta gaggacatca ggcgagtctt ccacca
//
LOCUS       DATMTI010000268         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_307781, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000268 DATMTI010000000
VERSION     DATMTI010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307781"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VN039_04485"
     CDS             <1..615
                     /locus_tag="VN039_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45276.1"
                     /translation="SVTRSPMKSPAYGSVWIVAVWTCGFACPSAAIEVQPAQIHIQLA
                     LDRGKEAAAHRQAPETFYTRFGSDEDLHAGGFLVTKLGGLSVLATHMALRGLEPSAAD
                     IAQVTEASTMLVSTVILGDSPSFAVNSYIVLDQGGKVVKPATVRVDGQAGRSSTWPKS
                     PKFRAKVVATFSYADFDPNAQTTITVFPATGGEVHFSLNFADID"
     gene            619..1908
                     /locus_tag="VN039_04490"
     CDS             619..1908
                     /locus_tag="VN039_04490"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HWV45277.1"
                     /translation="MARTSVTRLYTAETIAIGSELLVGGRSDGNSLFIADALAAIGIE
                     VRFKTIVGDDQSDIAGVLTTACRRAGIVIMTGGLGPTVDDCTREAVASVTGFRLARRK
                     EAFESMSARLAQWGRTPNRGQLRQALIPSGATVLTNSVGSAPGFRLTWRNAWVIALPG
                     VPSEMQAMMQQSVIPFLADRLDRSRQARPHPITRVTFHTWGLPESDVNTRLQGLIQKR
                     TPVALGLLASPTGVLVSLTTRAHRSIKEAVLLSLAEGVRTRLREWIYAEGQDTLEEVV
                     GRLLRQQKLTVAVAESCTGGLIGYRLTQIPGSSVYVDRGALCYSNQAKMDMLGVPAHL
                     IEQYGAVSREVAESMAKGMRDRAGASVALSVTGIAGPGGATETKPVGLVYIGLDGGGG
                     VMTKEFRFHGDRSVIRRRAAQAALDLLRQWLIARGEA"
BASE COUNT          445 a          526 c          632 g          438 t
ORIGIN      
        1 tctgttactc gcagtcctat gaaatctcct gcgtacgggt ccgtatggat agtggcagtc
       61 tggacatgcg ggttcgcgtg cccgtctgcc gcgattgaag tgcagccggc acagatacat
      121 atccagctgg cactcgatcg agggaaagag gcagcggcgc atcgccaagc tcctgagacg
      181 ttttatactc gattcggaag tgacgaagat ctgcatgccg gcggatttct tgtcacgaaa
      241 ctcgggggat tgtcggtcct ggcaacacac atggccttac gagggcttga accgagtgct
      301 gccgatattg ctcaagtgac tgaggcctcg acgatgcttg tgagcacggt gatccttggg
      361 gatagtcctt catttgctgt aaacagttat atcgtgcttg atcaaggtgg aaaggtggtc
      421 aagcctgcca ctgtgcgggt cgatgggcaa gccggtcgga gttcgacatg gcccaagtca
      481 ccaaaattcc gtgcgaaagt cgtagccacc ttcagctatg ccgatttcga tccgaatgcg
      541 cagacgacca tcacagtctt tcctgcgact ggcggagagg ttcacttctc gctcaacttt
      601 gctgatatag attaacccat ggcaaggaca tcagtaacac gcctatatac tgcggaaacg
      661 attgcgattg gttccgagct gcttgtggga ggccggtccg acggcaattc actgtttatc
      721 gccgatgcct tggccgccat cggtatcgag gtcaggttca agacaatcgt tggagacgat
      781 cagtcggaca ttgccggagt gctgacgaca gcctgtcgtc gggccgggat tgtgatcatg
      841 accgggggct taggtccgac ggtcgacgac tgcaccagag aagccgtcgc gtcggtaacg
      901 ggtttccgcc tggctcgccg taaggaagcg ttcgagagca tgagcgctag attggctcaa
      961 tggggacgaa caccgaatcg cggacaattg cggcaagcct tgattccgtc aggggcgaca
     1021 gtattgacca actcggttgg atccgcacct ggctttaggc tgacatggcg aaacgcgtgg
     1081 gtgatcgcac tgccgggtgt cccaagtgag atgcaggcca tgatgcagca atcggtgatt
     1141 ccgttcttgg cggatcgact cgaccgatcg aggcaggcac ggccccatcc catcacacga
     1201 gtgacctttc atacctgggg attaccggag tccgatgtga atacaaggct gcagggcctc
     1261 atacagaaac ggacgccggt cgcattgggg cttctggcct cgccgacagg agtgttggta
     1321 tcgctgacga cgagggctca tcgatcgatc aaagaagctg tacttctgtc attggcggaa
     1381 ggggtccgca cgagattgcg cgaatggatc tatgccgaag ggcaggacac gttggaagaa
     1441 gtggtcgggc gattgctacg acagcagaaa ctcacagtcg cggtagctga atcgtgcacg
     1501 ggaggtctta ttggctaccg actcacacaa ataccgggat cctcagtcta tgtcgatcgc
     1561 ggggcgcttt gctatagcaa ccaagcaaag atggatatgc ttggtgtgcc ggcgcatctt
     1621 atcgagcaat acggcgcggt cagccgagaa gttgccgaat ccatggccaa agggatgcga
     1681 gaccgcgcgg gcgcatcggt ggcattgagc gtaacgggca tcgccgggcc gggcggtgca
     1741 acagagacca aacccgtcgg gctagtctac atcggactgg acggtggtgg tggcgtcatg
     1801 acaaaggagt ttcgcttcca cggcgatcgg tccgtcattc gacggcgtgc tgctcaggcg
     1861 gcccttgatc tgcttcgtca atggctcatc gctagagggg aagcatgatc cggacttttc
     1921 ttgccgtgga attgggagat gatctccgaa accggatcac gctgattcaa caagacttga
     1981 gacaacgact cggctgcgaa tcctcaaaag atagtcggat tacttgggtg cagccgtcgt
     2041 c
//
LOCUS       DATMTI010000269         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_647139, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000269 DATMTI010000000
VERSION     DATMTI010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647139"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /gene="ttcA"
                     /locus_tag="VN039_04495"
     CDS             complement(<1..690)
                     /gene="ttcA"
                     /locus_tag="VN039_04495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HWV45278.1"
                     /translation="MIVPLAPTATPKLDEATEKIQTRLCRLVGQAIADYRLIEDGDKV
                     MVCLSGGKDSYGLLDILLVLQRRAPIRFDLIAVNLDQRQPGFPEHVLPQYLTSRGIPF
                     HIESRDTYSIVKRLIPEGQTTCSLCSRLRRGHLYRIATELGATKIALGHHRDDIIETL
                     FLNLFHTGKLKAIHPKLCSKDGRHVVIRPLAFVKEADLARYAELRGFPIIPCDLCGSQ
                     EELKRKKVKTFL"
     gene            763..1260
                     /locus_tag="VN039_04500"
     CDS             763..1260
                     /locus_tag="VN039_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45279.1"
                     /translation="MAPSILFVCTGNVFRSMAAEYALRAQLGRHSSYRVESAGTEAKP
                     KTIHPVISNRLIQKGTNPSAHIPQRLTKKLIDRNDLIIAMGLGHREFIHKQFGLKVPL
                     FNEVSFGEEIPILDLHEALPDWERDIDEARAYVESVIDHIWESVPALIARLPHFQEDR
                     RPMKS"
     gene            1410..>2295
                     /locus_tag="VN039_04505"
     CDS             1410..>2295
                     /locus_tag="VN039_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HWV45280.1"
                     /translation="MDRTVFDVLKTPVVGQDEPQWVSWSDDALLDLSMCDLHVGIKGG
                     FLEQPIAELIQELERRGLLFHPHFWLSNEWFTPDGVPGIAVPFYLAHPRLAKLEQAQM
                     LEVEGGTTEWCLRILRHEAGHAIENAYQIRRRKTRRHIFGKSSQRYPMYYSPRPYSRS
                     FVRHLDLWYAQSHPDEDFAETFAVWLTPDSLWEERYRGWPVLKKLKYVDGLMKELRGV
                     SPSVTTREEVDPLPSLKKSLREHYERKRRHYGIERQSQYDPDLKRLFSSLPSHASKLS
                     AATFLNRFRREVRRKVASW"
BASE COUNT          524 a          631 c          620 g          520 t
ORIGIN      
        1 aaggaacgtc tttacttttt ttcgcttcaa ttcctcttgt gagccgcaga ggtcgcaggg
       61 aatgattggg aatcctcgca gctccgcata tcgtgcgagg tctgcctctt tcacgaacgc
      121 cagcggtcgg atgaccacat ggcgaccgtc ttttgaacaa agcttggggt gaatggcctt
      181 cagcttgccg gtatggaaga gattcaagaa caaggtttca atgatgtcgt cgcggtggtg
      241 accgagcgcg atcttggtgg ctccaagctc cgtcgcaatg cgatagaggt gtcctcgtcg
      301 taaccgtgag cacaacgaac aggtcgtctg cccctctggg atgagtcgct ttacgattga
      361 ataggtatcg cgggattcaa tgtggaacgg aatgccgcga ctcgtcagat actgagggag
      421 cacatgttcc ggaaaacctg gttgccgttg gtcgagattg accgcgatca gatcaaaacg
      481 aatcggtgct cggcgttgca atacgagcaa gatatccagt aaaccataac tgtctttacc
      541 gcccgacagg cacaccatca ccttgtcgcc gtcttcgatg agccggtagt cggcgatggc
      601 ctggcccacc agccgacaga ggcgtgtttg tatcttctcc gttgcttcgt ctaatttcgg
      661 cgttgctgtt ggagcaagcg ggacgatcat ggcaagattg tagcggctcc cttatggtcc
      721 tgtcgacagg atcgcgcgta agaaaaggta agcagacctc ctatggcgcc gtcaattctc
      781 tttgtatgta ccggaaacgt cttccgcagt atggcggccg aatatgcatt acgggcacag
      841 ctcggccggc actcttccta ccgggttgaa tcggcgggga ctgaagccaa gcccaaaacg
      901 atccatccgg tcatctccaa ccggttaatc caaaaaggga ccaacccttc cgcacatatc
      961 ccacaaaggt tgaccaagaa actgatcgac cggaacgacc tcatcattgc gatggggctt
     1021 ggccaccgcg agttcatcca taaacaattt gggttgaaag ttcccctctt caacgaggtg
     1081 tcgttcggcg aggaaatccc gatcctcgac ctgcacgagg ccctccctga ttgggagcgt
     1141 gatatcgacg aagcgcgggc ttatgtggaa tcggtcatcg accacatttg ggaatccgta
     1201 ccggcgctga tagcacgact cccacatttt caggaagatc gacggccgat gaaatcgtag
     1261 cgtcgattca actcggaacc gtttgcacaa ttttctgctc gcctacctct cgaccatccc
     1321 tgagaagcct ccccatcgga cgggataaat attgcgtgct atattttcca tgaggggcac
     1381 aatgtgcgca tttcagaggt ttgcctttca tggacagaac ggttttcgat gtactgaaga
     1441 caccggtcgt cggacaggac gagccccaat gggtatcctg gtcggacgac gccctgttgg
     1501 acctctcgat gtgcgacctg cacgtgggaa tcaagggcgg atttcttgag caacccatcg
     1561 ccgaattgat ccaagaactg gagcgccgcg gattgctgtt tcatccacac ttctggctct
     1621 ccaatgagtg gtttaccccc gatggagttc ccggcatcgc cgtccctttt tacttggccc
     1681 atcctcgcct ggcaaaactg gaacaagccc aaatgttgga agtggaaggc gggaccacgg
     1741 aatggtgctt gcgcattctt cgtcacgaag cgggtcatgc gatcgaaaat gcctaccaaa
     1801 tccgccggcg aaaaactcgc cggcacattt tcggcaagtc ttctcaacgg taccccatgt
     1861 actactctcc ccgtccctat agtcgaagtt tcgtccgcca cttggacttg tggtatgccc
     1921 agagccatcc agatgaagac ttcgccgaga ccttcgcggt ctggctgaca ccggactccc
     1981 tgtgggagga acggtaccgg ggatggcccg tgctcaagaa gctcaagtat gtggatgggc
     2041 tcatgaagga actccgaggg gtttctccgt ccgtgacgac ccgcgaagaa gtcgatccgc
     2101 tccctagttt gaaaaaatcc cttcgcgaac attatgaacg caagcggaga cattatggaa
     2161 tcgaacgaca gtcgcagtac gacccggact taaaacggtt attttccagt ttaccgagcc
     2221 atgcgagcaa gctgagcgcc gcaacctttc tgaaccgctt ccgtcgagaa gtgcgacgaa
     2281 aagtggcgag ttgga
//
LOCUS       DATMTI010000270         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_647145, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000270 DATMTI010000000
VERSION     DATMTI010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647145"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2481)
                     /locus_tag="VN039_04510"
     CDS             complement(64..2481)
                     /locus_tag="VN039_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWV45281.1"
                     /translation="MPRDERFVEIHERSRRLPRWQVVLIGLVAVAIVGATTGAGIIWH
                     FSQDLPSLDLLQNYQPSLVTTVYSDDRQPIGQFFIERRILTPLPEIPKTLTQAVIATE
                     DARFFEHPGLDFIGMLRAAWTNIRHGGKKVEGASTITQQLARSLFLSSERSYERKIRE
                     LILAYKMEVVSGKEQILETYLNQIYFGQGAYGVGSAAHSYFDKDVRALTLAESAFLGG
                     LPKSPSRFSPFTAYELAKKRQEHVLARMEEVGFITAVEREAAAREKLNFHRPGSEHLA
                     PYFVEYVRQLLVAKYGESMVYKGGLQIYTTLNLEMQKAAESALLNGVRDLDKREGWRG
                     PRRTVDLATFQPSALAQADQLLKPGYLGEGVVIKVAKDHYVVQVGAFTAKLAFDDMAW
                     AKRILKGSDPAVDFVVNPNLKQLLKPGDVIEIGVKKLTKDGVQLALEQTPIVEGGLIA
                     IDPKGGAIRAMVGGYDFSRSEYNRAVQAHRQPGSAFKPLIYATAMSQGLSPATQILDA
                     PVVYEQEEDDKIWKPENYGRKFHGMVSLRDALAQSHNLATVRLLDKVGVKNVIEFSRT
                     LGVTSPLPADLSLGLGTSSVGLMELTSVYGVFLNQGSRVEPFAVKSVKDNMGKTLEVT
                     EPEPHEVISKETAYLITNMMEDVVQRGTGQAAKALGRPIAGKTGTTNDYINAWFIGGT
                     PNLVTGVYVGFDDRRSLGESETGARSALPIWNAFMKEALKQLPIVPFEIPDGVTFVKV
                     DAATGLLESEQEGEEQKGTVELFAKGSEPTQAVQRRLDPTDFYKLDQIPEGQPIGDDD
                     SLPPPGL"
     gene            2672..4528
                     /gene="typA"
                     /locus_tag="VN039_04515"
     CDS             2672..4528
                     /gene="typA"
                     /locus_tag="VN039_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWV45282.1"
                     /translation="MSIVRAPHDRRSDIRNIAIIAHVDHGKTTLVDAVLRQTHVHRKI
                     DDMGERIMDSMDQERERGITIRAKNASVIYNGVKINIVDTPGHADFGGEVERTLRMVD
                     GVLILVDAKEGPMPQTTFVLRKALALGHKAIVVINKIDRPDAVIDDVVNRTFDLFVHL
                     GATDEQLDFPIVYTSAIKGIATLDVNKPGTDIIPLLDTVLEKIPAPAITVEAPLQILV
                     LALVQDPYKGKMGIGKIQSGSIARRQNVMVLGKNGAQIPGKVSDLAVYSGLERADTEQ
                     AAAGEIVAVAGLDEVSIGDTIADAERPVALPRVSIDEPTVQMTFSVNNSPFAGREGKF
                     LTSRHLRERLFKELETNVSLRVNETDSADRFLVAGRGELHLAVLIEQMRREGYELQVS
                     QPEVILHREGGAVMEPYEELTIQVPENYQGTVIEEIGKRRGEMRHMRLIHSDVGTSEM
                     HLEYHIPTRSIMGLKNVLLAKTRGTVIMHHVFSAYEPAEERDLIVAPHGSLVAFEDGV
                     STGYAIFMTQERGAMFIGPGIDVYRGMVVGQNSRDEDLDVNVCKEKHLTNMRASGSDE
                     ALVLTPPREMTLEFALEYIGADELVEITPQNLRLRKRLLNPEDRRKARKAGK"
     gene            4545..5297
                     /locus_tag="VN039_04520"
     CDS             4545..5297
                     /locus_tag="VN039_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45283.1"
                     /translation="MRIRKKSPKPSAKRPPLYFVGYRGTAPSTEELKRLYEREYGAPL
                     AIRHEEGSAESWQAMQGPWSAHVVMPLPMSHVAEVMKQLSWEHELMGAVAPSISSPRD
                     MPDTVLLAARLARCLTLLTQGTAHDVITQAYVNPSDWQQCTLTSFHLDDHLSIIHDDT
                     SQPDLVWSYSLGLSKFGLDEVEVFMAKGLSDSTAKEVLIESACELLRVGHSPKVGTTL
                     DLPQLSRTIRIRNYRTAAPAGRMLGFRELQTS"
     gene            5604..6074
                     /locus_tag="VN039_04525"
     CDS             5604..6074
                     /locus_tag="VN039_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWV45284.1"
                     /translation="MSDVAPEIKVGDTAPDFNLKDQDQKDVKLSDYKGKKNVVLCFYP
                     LDWSPVCHGENKCLSDDFPKFQSANAELFGVSCDSFFSHKAWADSLDLKHRLLSDVHR
                     TTAKSYGLYFEPLNCSKRATVIVDKNGKVAYAKVQEIKTAREDKEILDALSKLN"
BASE COUNT         1393 a         1807 c         1722 g         1325 t
ORIGIN      
        1 tcgaacgaga agattctgga gacgattcag atggcttggc acgaggaata tctagattcg
       61 aagtcatagt cccggcggtg gtaagctgtc gtcgtctccg atcggttgac cttcggggat
      121 ctgatcaagc ttatagaaat ccgttggatc caaccgacgc tgcaccgcct gagtcggctc
      181 actccccttc gcaaagagtt caaccgttcc cttctgctct tctccttctt gctctgactc
      241 caggagtccg gtagcggcat cgactttcac aaaagtcacc ccgtcgggaa tctcaaacgg
      301 aacaatggga agctgcttaa gtgcctcttt catgaacgcg ttccagatcg gtaaggctga
      361 acgggctccc gtttcgctct ctccgagtga gcggcgatcg tcaaagccga catagacgcc
      421 ggtgaccaga ttcggtgttc caccgataaa ccaggcattg atgtagtcgt tcgtcgtgcc
      481 ggtttttccg gcgatcggac gccccaaagc ctttgccgcc tgcccggttc ccctctgcac
      541 gacatcctcc atcatgttgg taatcaaata agcggtttct ttggagatga cttcatgagg
      601 ttcgggctcg gtcacttcaa gcgtcttacc catgttatct ttgaccgatt tgacggcaaa
      661 gggttccact cgactcccct gattcaaaaa tacgccatat accgaagtca actccatcaa
      721 gccgaccgag gatgtgccga gtcccaggga cagatcggcg gggagcgggc tcgtgacccc
      781 gagggtacgc gagaactcga tcacattttt cacgccgacc ttgtccaaca accgaaccgt
      841 cgcaagattg tgtgactgag ccagtgcatc cctgaggctc accatgccgt gaaactttcg
      901 cccgtagttc tccggcttcc aaatcttgtc gtcctcttcc tgctcataga cgaccggggc
      961 atcgaggatc tgggtggcag ggctcaaccc ctgactcatc gcggtcgcat agatgagcgg
     1021 tttgaacgcg gaccccggct gccggtgagc ctgcaccgcc cgattgtatt cactgcggga
     1081 gaaatcatag ccgcccacca tcgcgcgaat cgcaccccct ttgggatcga tcgcgatcag
     1141 gcctccctcg acaatcggcg tctgctccag tgcgagttgc accccatctt tggtgagttt
     1201 tttcacgccg atttcgatga catccccggg cttcaggagt tgcttgagat tcggattcac
     1261 gacgaaatcc accgcgggat cagacccctt gagaatccgc ttggcccacg ccatgtcgtc
     1321 aaacgcgagc ttcgcggtga acgcgccgac ctgaaccaca tagtgatcct ttgcaacctt
     1381 tatgacgacc ccttcaccca gatacccggg tttgagcagc tgatccgcct gggccaaggc
     1441 tgagggctgg aaggtcgcca aatcgacggt tcgccgaggc cctcgccacc cttcccgttt
     1501 gtcgagatcg cgaaccccgt tcaagagcgc cgactccgcc gctttttgca tctccaaatt
     1561 caaagtcgtg tagatttgga gaccaccctt gtacaccatc gactccccgt acttcgccac
     1621 gagcagttga cggacatatt cgacaaagta cggcgcaaga tgttcgctcc cgggtcgatg
     1681 aaaattcagt ttttcacgag cagccgcttc ccgttccact gccgtaataa acccgacctc
     1741 ctccattcgg gcgagaacat gctcttggcg cttcttcgcc aattcatagg cggtgaacgg
     1801 agaaaaccgg ctgggcgact tgggcaggcc gcctaaaaat gcggattcgg ccagagtcaa
     1861 ggctctgacg tctttgtcga aataggaatg agccgccgag ccgaccccgt aggccccctg
     1921 cccgaagtag atttggttca agtaggtttc taggatctgc tccttcccgg agaccacctc
     1981 cattttgtac gcgagaatga gctcacgaat tttccgttca tacgaccgct cggacgagag
     2041 aaaaagcgaa cgggccaact gttgtgtaat cgtgctggcg ccttcgactt tctttcctcc
     2101 atgtcgaatg ttcgtccagg ccgcccgcag catcccaata aagtccaacc ccggatgttc
     2161 aaaaaatcgc gcgtcttccg tcgcgatcac cgcttgagtc agcgtttttg gaatttcagg
     2221 gagcggagtc agaatgcggc gttcgataaa gaactggccg atgggctggc gatcatctga
     2281 atagacggta gtgaccaaac tcggttgata gttttggagc aggtcgagcg atggaagatc
     2341 ttgagaaaag tgccagatga ttcctgcgcc cgtcgtggct cccacaatgg cgacggccac
     2401 gagaccgatc aagacgacct gccagcgagg caagcggcgt gaccgttcat gaatttcgac
     2461 gaaccgttca tcgcgtggca ttacaattca ctcctgagga agtccctctg tggggctgga
     2521 aaaccttaca atgcgccctt tcaaaactca agtttccacc gtgaaagtca tctgaatgat
     2581 cgcttgagag acggtcggcg ctcatgtata ctttcgccga cgtctcggta gcggggcgtc
     2641 ttttttttgt cattaaggaa gtaccgtcat tatgtccata gtacgcgcgc ctcatgaccg
     2701 tcgcagcgat attcggaata tcgccattat cgcccacgtc gatcacggca aaacgacttt
     2761 ggtcgatgcg gtgctccgcc aaactcacgt ccatcgcaag atcgacgata tgggcgaacg
     2821 catcatggac tcgatggacc aggaacgaga gcgtgggatc acgatccggg ctaaaaacgc
     2881 cagcgtcatc tacaacgggg tgaaaatcaa cattgtcgat actccaggcc atgccgattt
     2941 cggcggtgaa gtggagcgga cgctccggat ggtggacggt gtattgattt tggtcgatgc
     3001 gaaagagggc cccatgccgc aaacgacctt cgtgttgcgg aaagccttag cgcttgggca
     3061 caaagccatt gtcgtcatca acaagatcga ccggccggac gccgtcatcg acgacgtcgt
     3121 caaccgcacc ttcgacctat tcgtccatct gggagccacc gacgaacaac tcgactttcc
     3181 gatcgtctac acgtcggcga ttaaagggat cgccacgctc gatgtcaaca agccgggtac
     3241 cgacatcata ccgttactcg ataccgtgct ggagaagatt ccggcccccg ccattaccgt
     3301 cgaggccccg ctccaaattc ttgtgctggc ccttgtgcaa gatccctaca aaggcaagat
     3361 ggggatcggc aagatccagt ccggttcaat tgcccggcgg cagaatgtca tggtcctcgg
     3421 caagaacggc gcgcagattc caggaaaagt gtccgacctc gcggtctatt cgggccttga
     3481 acgagccgac accgaacagg ccgccgccgg agagatcgtc gcagtggcgg ggcttgatga
     3541 ggtgagcatc ggcgatacca tcgccgacgc cgagcgtccg gtcgccctcc cgcgcgtctc
     3601 gatcgacgag ccgacggttc aaatgacctt ctcggtgaat aacagtccct tcgccgggcg
     3661 tgaaggcaag ttcctgacat cacgccattt gcgtgagcgc ctgttcaaag aactggaaac
     3721 caatgtatcg ctccgtgtca atgaaacgga cagtgccgac cgcttcctgg tggcgggccg
     3781 aggcgagctc catctcgccg tgttgatcga acagatgcga cgggaagggt acgaacttca
     3841 agtctcgcaa ccggaagtca ttctccatcg tgaaggcggc gccgtcatgg agccgtacga
     3901 ggagttgacc atccaggtgc cggaaaacta tcagggcacg gtcatcgaag agatcggcaa
     3961 acgccgaggc gagatgcggc acatgcggct cattcactcc gatgtcggca ccagtgagat
     4021 gcacttggag taccacattc cgacccgaag tatcatgggg ctaaagaacg tgctcctggc
     4081 gaagacccgg gggaccgtca ttatgcacca tgtcttttcg gcttacgaac cggcggaaga
     4141 gcgggacctg atcgtggctc cacatggctc gcttgtcgcc ttcgaagacg gtgtcagcac
     4201 gggctatgcc atcttcatga cccaggaacg cggtgccatg ttcatcgggc ccggcatcga
     4261 cgtctaccga gggatggtcg tgggccagaa cagccgagat gaggatctgg atgtgaacgt
     4321 gtgcaaggag aagcacctca ccaatatgcg ggcctccggt tccgatgaag ccttggtgct
     4381 tacacctccg cgcgaaatga cgctggaatt tgctttggaa tacatcgggg ccgatgagtt
     4441 ggtcgagatc acccctcaga accttcgtct gcgtaaacga ctgctgaatc cagaagaccg
     4501 ccgcaaagca cggaaagccg gaaagtgatt caacaagcat tagaatgaga atccggaaaa
     4561 agtctcccaa accttcggcg aaacgccctc ctctctattt cgtcggctat cgtggcactg
     4621 ccccatccac cgaggagctg aagcgtctgt atgagcggga gtacggcgct ccattggcca
     4681 tccgacatga agaggggtcg gccgagtcct ggcaggcaat gcaggggcct tggtcggccc
     4741 acgtcgtgat gccgttgccg atgagccatg tcgccgaggt gatgaagcag ttgtcgtggg
     4801 aacacgagct catgggagcc gtcgcaccgt ccatctcctc tcctcgcgac atgcccgata
     4861 ccgtcctact cgccgcgcga ctggcccgtt gtctgactct tctgacacag ggcactgccc
     4921 acgacgtgat cacacaagcc tacgtcaatc cctcggactg gcaacaatgt accctcacga
     4981 gttttcatct ggacgaccat ctctcgatca tccacgacga cacctcgcaa ccggacctgg
     5041 tgtggtctta ttcactgggc ctgagcaaat tcgggctgga cgaggttgaa gtgtttatgg
     5101 cgaaaggact ttccgacagc acggcgaagg aggtgctgat agagtcggcc tgcgaattgc
     5161 tgcgggtggg acattctccg aaagttggaa caacgctcga ccttcctcag ctgagtcgca
     5221 cgatccgcat caggaattat cggaccgccg caccagccgg tcgaatgttg ggattccgag
     5281 aacttcaaac ctcgtaggtc cgtcgataac tcaatacgtt atcgaattct cgtctgacgc
     5341 cagtcgccca cgggctgttc aaaaagttcg ttcagcaagg ccgcagcgag cgaaggggcg
     5401 aggcgtatgc cttgatacgt tgagcctctg agcgatgcga gaacgccgct ggcggacttt
     5461 ttcaacagcc gttaggcccg tcagaccgtt gacatccccg taaaccacag gttacaaagt
     5521 ttatcgtgca tggtggggtc ctgccaattc gagacctcag tatagcaatc acccatacag
     5581 acgtttaatg aaggaggttt gcgatgagcg acgtggcacc ggaaattaag gtgggcgata
     5641 cagcgccgga cttcaatctg aaggatcaag accagaagga tgtgaagttg agcgactaca
     5701 aaggcaagaa gaatgtcgtg ctctgcttct atccgctgga ttggagcccg gtgtgccatg
     5761 gagagaacaa atgtttgtcc gatgatttcc ccaagttcca gtccgccaac gcagagttgt
     5821 tcggcgtcag ctgcgacagc tttttttccc acaaggcctg ggcggattct ttggacctca
     5881 agcaccgcct gttgtccgac gtgcatcgga cgacggccaa gtcgtatggc ctctactttg
     5941 aaccgctgaa ctgttccaag cgcgcgaccg tgatcgtcga taagaacggc aaagtcgcct
     6001 acgcgaaggt gcaggaaatc aagacggcac gagaggacaa ggagatcctc gatgcgctgt
     6061 cgaagctgaa ctgatcactg gttctgagtg ctgggtgctg agggtcgagc aatcggataa
     6121 ctcagcactc ggcgctcaac gctcagcact gcttatgagc accgttctcg acgtcagcga
     6181 cgccaactac aaagagttca ccgacagcgc cggtgcggtc gtcgcgtacg gccttgccac
     6241 ctgcgat
//
LOCUS       DATMTI010000271         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_95059, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000271 DATMTI010000000
VERSION     DATMTI010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95059"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VN039_04530"
     CDS             <1..955
                     /locus_tag="VN039_04530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWV45285.1"
                     /translation="AILSAAGYENILTADSAAAAFRHLGLDGGKQGAARVDLILMDIL
                     MPQMNGIEACRQIKAADRFRDTPIIMVTVKTDPVDLQLAFAAGAMDYVAKPVNKVELL
                     TRVRSVLRLVHEIDRRRAREQELLEVMRQLQEANQMLLRLSCLDGLTGITNRRQFDDF
                     LDQEWRRAARESTPLSLIMFDIDRFKTYNDAKGHTSGDECLKQVAAAISSSVNRPGDL
                     VARYGGDEFVTVLPGTGIDGAAQVAESLRRRVESLGMKHADGELVTISVGYASIVPNR
                     NASPTDLIKTADQALYQAKQEGRNRIKSASILSLVQDSHGD"
     gene            992..3277
                     /locus_tag="VN039_04535"
     CDS             992..3277
                     /locus_tag="VN039_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45286.1"
                     /translation="MSRSRYPHGGPQDHAAVTMISSRPSRPTTRRTTASLKRKPSERT
                     ALPFEQSATMRLKQYQETLRRLAQSEALNSGNISRVFRIISETCCSLLQVKRASVWLF
                     TEERTAIELIDVCTSGRRHSNPTFVLQSSQWLVYLRSLTRKNRRIAVHHAFRDSRFKE
                     LAPSYASRFHVEAVLSAPIRQNRRLVGALCAESVGAPRRWSRHEEHLVGLLATMTTLA
                     LEAAERHEVEQALRVAKEAAEVANRAKSEFLASMSHEIRTPMNAIIGMADLLWDTPLS
                     PDQRKYLRIFRRAGGTLLNLINDILDLSKVEAGRLELESIEFDLNEVIDKAIDIMAMR
                     ANEKGLELACHVDQNVPCHLVGDPTRLTQILINLIGNALKFTDEGSIVVRVTNDLDRN
                     IAGAVRFSISDTGIGIPSDHLVSIFERFTQAHASTTRQYGGTGLGLAITKRLVEGMNG
                     RIWAESTMGTGSTFYCSVLLEAQTESSHQKSATPINLDGVRVLVVDDHPTNRLILREL
                     LSAWGAQVTEASDGMAALDELRQASERGHAYELLLLDCRMPEMSGFRIVEQLKSSPNG
                     GSPTIIMLTSDHWADDIARTYDLGLGGYLIKPIRRSDLSQTIGIALGRTKGTPQTGVE
                     VIASASAVSTRALRVLLVEDSPDNQLLVRAYLKQTDYVLDVAEHGAVALDKFKSAHYD
                     VILMDVQMPVMDGYAATKAIRSWERDHDLPPIPIVALTALALKEEGARIFEAGCNTHL
                     TKPIKKSTLLEVLRAYKEHIV"
     gene            3280..3603
                     /locus_tag="VN039_04540"
     CDS             3280..3603
                     /locus_tag="VN039_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWV45287.1"
                     /translation="MQASHPEPTDQFTVEISRDLEGIVPIFLGNRKKDARILRDALVK
                     QDFRTVQTLGHRMKGDGGGFGFDRITEIGAAMERAAKLEDPLTIEEHIGQLEDFLQRV
                     TVVYR"
BASE COUNT          893 a         1106 c         1020 g          812 t
ORIGIN      
        1 ggccattctg tcggctgccg gctatgaaaa cattctgacg gcggattccg ccgccgccgc
       61 gttcagacat ctcggcctgg acggaggcaa gcaaggcgct gcccgggtcg atctaatcct
      121 catggatatt ctcatgccgc agatgaacgg catcgaagcc tgtcggcaga taaaggccgc
      181 cgaccggttc cgagataccc caatcattat ggtcaccgtc aagacggatc cggtcgatct
      241 tcaacttgcg tttgccgccg gagccatgga ttacgtcgcc aaaccggtca acaaggtcga
      301 gctcctgacc agagtacggt ctgtgctccg gcttgtccac gaaatagacc gccgccgcgc
      361 ccgggagcag gaactccttg aggtcatgcg tcaactccaa gaagccaatc agatgttgct
      421 ccgtctttcc tgcttggatg gattgacggg tatcaccaat cgacgtcagt tcgacgactt
      481 tttggaccag gaatggcgga gagcggcgcg cgagtcgact cctttgtcgc tgattatgtt
      541 cgatatcgac cgatttaaga cctataacga cgccaaaggt catacgtccg gcgatgagtg
      601 tctcaaacag gtcgctgcgg caatctccag ctcagtgaat cgccccggtg atttggtcgc
      661 ccgctatggc ggagacgagt ttgtcacggt actgcccggc acaggtatcg acggtgcggc
      721 acaggtagcg gagagcctcc gtcgccgagt cgaatctctc gggatgaaac acgcagatgg
      781 agaactcgtc acgatcagcg tcggatatgc cagcatcgtt cccaacagaa acgcttctcc
      841 tacggatctc atcaaaactg cggatcaggc tctctatcaa gcgaaacaag agggacgcaa
      901 tcggatcaaa tcggccagta ttttgtcgct ggtccaagac tcacacggag actagaggcg
      961 ctcagaacta ttcgccgagt attgattccc catgtcacgg tcgcgctatc cacatggagg
     1021 acctcaagat cacgccgcag tcaccatgat ctcctctcgg ccttcccgcc cgacaaccag
     1081 acgaaccacc gcttccctca agcggaagcc ctccgaacga accgcccttc cattcgagca
     1141 gagtgcgacc atgcggctca agcagtatca agagacgctc cgcagactgg cgcaaagcga
     1201 ggcgttgaac agcggcaata tttcccgtgt ctttcggatc atttcagaga cgtgctgtag
     1261 cttgttacaa gtcaaacgcg ccagcgtctg gctgtttact gaggagcgaa ctgccatcga
     1321 actgatcgac gtgtgcacgt ccggccggcg acattccaac cctaccttcg tgctccagtc
     1381 ctcacagtgg cttgtttatc ttcgatcgct gacccgtaaa aatcgccgca ttgcagttca
     1441 ccatgctttc cgagattcac gattcaaaga gctggccccg tcgtatgcct cgcgcttcca
     1501 tgtcgaggcc gtgttgagtg caccgattag gcagaataga cggctcgtgg gcgcactctg
     1561 tgccgagtcg gtcggcgccc ctcgacggtg gagccggcat gaagaacacc ttgtcggcct
     1621 actcgcaacc atgacaaccc ttgctttaga agcagccgaa cggcatgaag tagaacaagc
     1681 cctccgtgtc gccaaagaag ccgctgaagt ggccaaccgc gcgaaaagtg aatttctcgc
     1741 cagtatgagt catgaaattc gcacccctat gaatgccatc atcggcatgg ccgatctcct
     1801 ttgggatact ccgctctcac cggaccaaag aaaatacctg cgtattttca ggcgtgcggg
     1861 cggcactctt ttaaatctta tcaacgatat tttggatctt tccaaagtcg aggccggtcg
     1921 cttggagttg gaatccatcg aattcgacct gaacgaagtc atcgacaagg ccatcgatat
     1981 catggcgatg cgtgccaatg agaaggggct ggagcttgcc tgtcatgtgg atcaaaatgt
     2041 gccctgtcat ttggtcggtg acccgacgcg gctgacacag atactgatca atttgatcgg
     2101 caatgccctc aaatttaccg acgaaggttc cattgtcgtt cgtgtcacga acgatctcga
     2161 ccggaatatc gccggtgccg ttcggttttc catcagcgac acgggaatcg gcattccttc
     2221 cgaccatctc gtttcgattt tcgaacgttt tacgcaggca catgcctcaa cgacccggca
     2281 gtatggaggg acaggactcg gccttgcgat caccaaacgc ctcgtcgaag gtatgaacgg
     2341 gcggatctgg gctgagagta caatgggaac gggaagcacc ttctactgtt ctgtcctgct
     2401 tgaggctcaa acagaatcgt cccatcagaa atccgcaaca ccgatcaatc ttgacggagt
     2461 cagagtcctg gtcgtggacg accatcccac caaccgcctc atactccgtg aactgctgag
     2521 tgcctggggg gctcaggtta cggaagcaag cgacggaatg gccgcattag acgaacttcg
     2581 ccaggcatcc gaacgagggc atgcgtacga gcttctgctc ctcgattgcc ggatgcctga
     2641 gatgagcggt ttccgtatag tggaacagct gaaatcctcg cccaacgggg gctctccgac
     2701 catcatcatg ttgacctccg atcattgggc ggatgatatc gcacggacgt atgatctcgg
     2761 cctcggtggg tatctgatta aacccatccg gcggtccgat ctttcgcaaa caatcgggat
     2821 cgccctcggc cggacaaaag gaacgccgca gacgggtgtg gaagtcatag catccgcgtc
     2881 ggccgtttcg acgcgggcct tgcgcgtatt attggttgaa gattcccccg acaaccaact
     2941 actggtccgg gcatacctga agcagaccga ctatgtcctc gacgtggcag aacacggcgc
     3001 cgtcgcactg gacaaattca agtccgcgca ttacgacgtc atcttgatgg acgtgcaaat
     3061 gcctgtgatg gacggctatg ccgcgacaaa ggccatccgc tcatgggaac gggatcatga
     3121 tcttccaccc ataccgatcg tcgccctcac tgcattggcc ctcaaggagg aaggagcacg
     3181 catctttgaa gccggctgca acacacacct cacgaagccc ataaagaaat ccacgttatt
     3241 ggaagttctt cgggcgtata aggaacacat cgtctaacaa tgcaggcctc ccatccggaa
     3301 ccaacagatc aattcacggt ggagatcagt cgcgatcttg aagggattgt gccgatcttc
     3361 ttaggtaacc ggaagaaaga tgcccggata cttcgagacg ctttggtcaa gcaagacttc
     3421 aggaccgttc agacgctggg ccatcgcatg aaaggcgatg gaggcggctt cgggttcgat
     3481 cgaattaccg aaatcggagc ggcgatggaa cgcgcggcaa aactggagga ccccttaaca
     3541 atcgaagagc acatcggaca gctggaagat ttcctccaac gtgtgaccgt cgtctaccgc
     3601 tagagaatag cggcctgttt caaccgaccg actaccagca tcggcttccc acagcagttt
     3661 ccagatcgtc agccattcga atggttcctc atcatcaagc cataattgga cgttccggag
     3721 gaggtgatcg ctgcaacaca tagtcgatcg aaagcatcag tcgcttggtc tgacatcggc
     3781 tggccttatg aaaagcgctt gtttcctcga acaccatggt ccccttttcg c
//
LOCUS       DATMTI010000272         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_434636, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000272 DATMTI010000000
VERSION     DATMTI010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434636"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..716
                     /locus_tag="VN039_04545"
     CDS             132..716
                     /locus_tag="VN039_04545"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HWV45288.1"
                     /translation="MGNHPCLDLINTQLIAKGEPRDLLESVDDLSLWLRQAKLLTASQ
                     AKIAKVHLHNEEAASLLERTKTLRATFRIVAERVAAGKAVPDSAIDATNQLLAQRPGY
                     TRLVRTKEGVKRQFHSLATHQVEFLASLLESASDLLCSGRLCLIKKCANAACILYFLD
                     TTKNHTRNWCSMQMCGNRMKVAAHYRRSRGKASL"
     gene            890..1429
                     /gene="atpH"
                     /locus_tag="VN039_04550"
     CDS             890..1429
                     /gene="atpH"
                     /locus_tag="VN039_04550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HWV45289.1"
                     /translation="MIKTTVARRYAQALFELLDQSAIEATRGTLTGLGQAIKESDQLR
                     HVVASPAFGVEEKIAVLTALGDTLGCPPTGKAFLGQLVKKNRVGFLSEIADAFGKLVD
                     ESKRTQPVTVSSATDLSPAEQDRIRTRLRETLKREVDVTFQTDAGHLAGLQIHIGSTV
                     VDSTVRGRLSDLQVLLTRE"
     gene            1439..2959
                     /gene="atpA"
                     /locus_tag="VN039_04555"
     CDS             1439..2959
                     /gene="atpA"
                     /locus_tag="VN039_04555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWV45290.1"
                     /translation="MQIRAEEISAIIKEKIKGFDKQVDVKETGSVIQVGDGIAKVYGL
                     DGAMAGEMLEFPGGLYGIALNLEEDNVGAVLMGEDVGIKEGDPVKRTGRIAEIPVGEA
                     LVGRVVNAIGQPIDGKGPIKSQHSSRIEVVAPGVNTRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGLNVFCIYVAVGQKRSTVARVVKTLEENHAM
                     EYSMVVSASASDPAPMQFLAPFAGAAIGEYFRDNGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGSDLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFSQFGSELDKATQMQLARGVRMVELLKQGQYKPMPVADQVLSIYAGVNGFLDDVPV
                     DKVQQFEGDLLHYIQQNHPDLKKEVTTIGKIDDKVGGRLKEIITTFKQKMGYGAKS"
BASE COUNT          732 a          876 c          920 g          631 t
ORIGIN      
        1 gtactaacct tctaagtata atttagatgg ttatatcaac ggaaggctaa cttgtcaacg
       61 ggctctttgt aggttatcta taaaccgagg caagagaacc gatgaagagg gaagcacgaa
      121 ggtttctgtt cgtgggcaac catccttgtc tggatttgat caatacccag ctgatagcga
      181 aaggagagcc gagagatctt cttgagagcg ttgatgacct ctctttatgg ctgaggcagg
      241 caaaacttct tacagcgtcc caggccaaaa tcgccaaagt tcatctgcac aatgaagaag
      301 ccgcgtcctt gcttgagcgg acgaagacct tacgggcgac gtttcgaatc gtcgccgaac
      361 gggtcgcagc gggcaaagct gtgcccgatt cagccatcga cgccacgaat cagttgctgg
      421 ctcagcgtcc cggttacacc cggcttgttc ggaccaagga aggcgttaag cggcagttcc
      481 actctctggc gacacatcag gtagaattcc tggcttctct tttggaatcg gcgagcgatc
      541 tgctctgttc cggccgtttg tgtttgatca agaaatgcgc caatgccgcc tgtattcttt
      601 attttttaga tacgaccaag aatcacacgc gcaactggtg cagcatgcag atgtgcggta
      661 atcggatgaa ggtggcggcg cactaccgga gaagccgggg taaggcaagt ctctaaacgc
      721 cggcagcggc gtaccacaga tgcagtgccg ccgttgacga gcatctcgcc cgaccgtgat
      781 catatcgtat tgacagctcc taggtcccaa tgctaacttt ccgcgctctg cttcgtgcct
      841 cgcacgaact ctttacgttt gtccaaagaa cgcatgtgga tctggaacgg tgattaagac
      901 aacagttgcg cggcgttacg ctcaagccct cttcgagctc ctcgatcaat cagccatcga
      961 agccacacgg gggacgctca ccggcctcgg tcaggccata aaggagtcag atcagcttcg
     1021 ccacgtggtg gcctctcctg cgttcggggt ggaggagaag atcgccgtcc tgaccgcact
     1081 cggcgatacg ctagggtgtc ctccgaccgg taaagcgttt ttggggcagt tggtgaagaa
     1141 gaatcgggtg ggctttctgt cggaaatcgc cgacgcattc ggcaagttgg tcgatgaatc
     1201 aaaacggacg cagccggtga cggtttcctc cgcgacggat ctttcaccgg cggaacaaga
     1261 tcgaatcagg acacgcctac gagaaacgct caagcgcgaa gtagatgtga cgtttcagac
     1321 cgatgccggt cacctcgccg gcttgcagat tcacatcggc agcacggtgg tcgacagcac
     1381 cgtccgaggc cgcttgagcg atttgcaagt cctgttgacg cgagagtaag gagtagccat
     1441 gcagatcagg gcagaagaaa tcagtgcgat catcaaagag aagatcaaag gctttgataa
     1501 acaggtcgat gtcaaggaga cagggtccgt cattcaggtc ggcgacggca tcgccaaggt
     1561 ctatggcctc gatggagcca tggccggcga gatgctcgaa ttccccggcg gcctctacgg
     1621 tatcgcgctg aatctcgaag aagacaatgt cggcgccgtg ttgatgggcg aggatgtcgg
     1681 catcaaagaa ggcgatccgg tcaagcgtac gggccgcatc gcggaaattc cggtcggcga
     1741 agcgctcgtc ggtcgcgtcg tgaacgccat cggtcagccg atcgacggca agggcccgat
     1801 caagtctcag cactcttccc gcatcgaagt cgtggcgccc ggcgtgaaca cccgccaatc
     1861 cgttcgcgag ccgctgcaga ccggcatcaa agccatcgac gccatgatcc ccatcggccg
     1921 cggccagcgc gaattgatca tcggcgaccg ccagaccggg aagaccgcca ttgcggtcga
     1981 tacgatcatc aatcaaaaag gcctgaatgt cttctgtatc tatgtcgccg tcggccaaaa
     2041 gcgctcgacc gtcgcgcgag tcgtgaaaac gctcgaagaa aaccatgcca tggagtacag
     2101 catggtggtc tccgccagcg ccagcgaccc ggcgccgatg caattcctgg cgcccttcgc
     2161 gggcgcagcc atcggggagt atttccgcga caacggcaag catgcgctga tcgtgtacga
     2221 cgatttgtcg aaacacgccg tcgcctatcg ccaactttcg ttgttgctcc gccggccgcc
     2281 gggacgcgaa gcctatcctg gcgacgtgtt ctatctccac tcacggttgc tcgaacgcgc
     2341 ggccaagcta agcgacaaac tgggcggagg cagtttgacg gcgcttccca tcatcgaaac
     2401 gcaagccggc gacgtgtcgg cctacattcc aacgaacgtc atttcgatca cggacggtca
     2461 gatctatctg gggagcgatc tcttttactc cggtatccgt ccggcgataa acgtcggtct
     2521 gtcggtctcc cgggtcggcg gctccgcgca gatcaagacc atgaaacagg tggccggtac
     2581 ccttcgattg gatctcgctc agtatcgtga aatggcggcg ttttcccagt tcggcagcga
     2641 gctggataag gcaactcaga tgcagctggc gcgtggtgtg cgcatggtgg aattgctcaa
     2701 acaggggcag tataagccga tgccggtagc cgatcaggtt ctctcgatct acgcgggtgt
     2761 caacggattc ctcgacgacg tgccggtgga caaggtgcag cagttcgagg gcgatttgct
     2821 ccactacatc cagcagaacc atccggatct gaagaaggaa gtcaccacca tcggcaagat
     2881 cgacgacaag gtcggtggtc gcttgaaaga aatcatcacc accttcaagc agaagatggg
     2941 atacggagcg aaatcgtaag cataggactg agtatcgagg actcaggact gaggcggacg
     3001 gtggggagcc gagttgcaac ctcagtcctc agcactcagt ccacagtcct gatggaatac
     3061 aatgccaagc cttcaatcgt tgcgcagaaa gatagcggcc ttcaagaata cccagaagat
     3121 caccaaggcc atgaagatgg tggccgcggc gaagctcaa
//
LOCUS       DATMTI010000273         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_350074, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000273 DATMTI010000000
VERSION     DATMTI010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350074"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="VN039_04560"
     CDS             <1..1989
                     /locus_tag="VN039_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWV45291.1"
                     /translation="EIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYL
                     TRRLVDIAQDVIINEIDCGTRDGIIVSALVEGGEIIQPLEERVLGRLAAEDIRDPVTG
                     EIIVSWNEEINEELTKSIVEAGVDRVKIRSVLTCQSPRGVCRACYGRDLARGRLVEKG
                     EPVGVIAAQSIGEPGTQLTMRTFHIGGTASKVVEQTVLEAKHAGRIKFMSFDAKKNAD
                     IHNAGIAVRNKEGEWVVMNRNTKIAIVDDSGREREKYPVVYGAKIKIKDGDPVVVGQK
                     LVEWDPYSLTILTEVGGRVAYGDIVEGVTMKDEFDEVTGLSRKVIIEHTGQMLRPRVS
                     IKDESGKTAKVPGGSNAVARYLLPVGAHIFVEKGATVYPGDVLAKIPRETTKTKDITG
                     GLPRVVELFEARKPKEQAVITEIDGEVSYGGFVKGQRKVLVDNKMGDVKEYFIPKGKH
                     VNVHEGDWVRAGEPLMDGSANPHDILDVLGPNELQKYLVDEVQDVYRLQGVSINDKHI
                     EIIVRQMLRKVRVEDPGDTQFLPGSQVSKSVFEKENERVFANDGKPALGKPVLLGITK
                     AALTTDSFISAASFQETTRVLTEAAINGREDNLLGLKENVIVGRLIPAGSGFEEYRDT
                     FVISEKSEGTPVGVSPAAPAVPSDAAVVPAVSGEAARS"
     gene            2099..2470
                     /gene="rpsL"
                     /locus_tag="VN039_04565"
     CDS             2099..2470
                     /gene="rpsL"
                     /locus_tag="VN039_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWV45292.1"
                     /translation="MPTINQLVRKGRIFVKAKTKSPALKSCPQKRGVCLRVYTATPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHTLQEHSIVLVRGGRVKDLPGVRYHLVRGAL
                     DAVGVADRKQSRSKYGAKRPK"
BASE COUNT          602 a          574 c          767 g          535 t
ORIGIN      
        1 gagatcatcg aaacgcccat cacagccaat ttccgtgaag ggttgacggt gttgcagtac
       61 ttcatctcaa cccacggtgc tcgtaagggt cttgccgaca cggcgctgaa aaccgccaat
      121 tcaggttatc tgactcggcg attggtcgat atcgcccaag acgtcattat taacgagatc
      181 gattgtggaa cgcgggacgg tattatcgtc agtgccttag tggaaggcgg tgaaattatt
      241 cagccgttgg aggaacgcgt cctcggtcgt ttggcggcgg aagatattcg tgaccccgtc
      301 acgggggaaa tcatcgtgtc ttggaatgaa gaaatcaatg aagaattgac gaagtcgatc
      361 gttgaggccg gagtcgaccg tgtcaagatt cggtccgtgc tgacctgtca atcgccgcgt
      421 ggtgtctgtc gtgcctgtta cggccgcgat ctggcacgag ggcggctggt ggaaaaaggc
      481 gaaccggtcg gtgtcatcgc cgcgcaatcc atcggcgagc cgggcaccca gctgacgatg
      541 cggacctttc acatcggcgg cacggccagt aaggtcgtcg agcaaacggt gcttgaggcg
      601 aagcatgccg gacgaattaa gttcatgagc ttcgatgcca aaaagaatgc cgatatccac
      661 aatgccggta ttgcggtgcg gaataaagag ggtgaatggg tcgtgatgaa tcgcaatacg
      721 aagattgcga ttgtcgacga cagcggacga gaacgtgaga agtaccccgt ggtgtatggg
      781 gcgaagatca agattaaaga cggcgatccg gtggtcgtcg gccagaaatt agtcgagtgg
      841 gatccgtact cgctgacgat tttgacggag gtcggtggga gggtggcgta cggtgatatc
      901 gtcgaaggcg tcacgatgaa ggacgagttc gacgaagtga ccggcttgtc gcgcaaggtc
      961 atcattgaac ataccggtca gatgcttcgc cctcgtgtgt cgattaagga tgaaagcggc
     1021 aaaacggcca aggtgcccgg cggatccaat gccgtggcca ggtacttatt accggtcgga
     1081 gctcatatct tcgtcgaaaa aggggccacg gtctatcccg gtgatgtctt ggcaaagatt
     1141 cctcgtgaaa cgactaagac caaggacatc acgggaggtc taccgcgcgt ggtggaactt
     1201 ttcgaggcaa ggaaaccgaa agaacaggcg gtcatcaccg agatcgacgg agaagtttcc
     1261 tacggcggtt tcgtgaaagg ccaacgcaaa gtccttgtcg acaataagat gggcgacgtg
     1321 aaggaatatt ttattcccaa aggcaagcat gtcaatgtcc atgaagggga ctgggtgcgg
     1381 gctggggagc cgttgatgga cggatcagcg aatccgcatg acatccttga tgtgttgggt
     1441 ccgaacgaat tgcagaagta ccttgtggac gaagtccaag acgtgtatcg attgcaagga
     1501 gtttcgatta acgacaagca catcgagatc atcgtgcggc aaatgttgcg gaaggtacgg
     1561 gtcgaagatc cgggagacac ccagttcttg ccgggcagtc aagtcagcaa gagcgtcttt
     1621 gaaaaagaaa acgaacgggt attcgccaac gacgggaagc cggccctggg caagcccgtc
     1681 ttgcttggga ttacgaaggc ggctcttacg acagacagct ttatttctgc agcatcgttc
     1741 caggaaacca cccgcgttct cactgaagca gcgatcaatg gacgcgaaga taatttgctg
     1801 ggcttaaaag aaaacgtcat tgtaggccgg ttgatccctg cggggtcagg atttgaagag
     1861 tatcgggata cgttcgtcat tagcgaaaaa tcggagggga cacccgtagg tgtttcacct
     1921 gcagcacctg ctgtgccatc tgatgcggcc gttgtcccgg cggtttcagg agaagccgct
     1981 cggtcttagt taatcgctga aaggctgcct acttgacagt gggcggttac ctcactataa
     2041 tcctgcggct ttgcacttga cggttggtgc ttgttccttt tgaaagggtt taaacgcgat
     2101 gccgacgatc aatcagctgg ttcgaaaagg gcggatcttt gtaaaggcaa agacgaagag
     2161 tcctgccctc aagtcgtgtc cgcagaagag aggcgtttgc cttcgcgtat acacggcgac
     2221 accaaagaag ccgaattcgg cattgcggaa agttgcgcgt gtgcgtctca caaacggtat
     2281 ggaagtgaca acgtatatac ctggcgtggg gcacactctc caggagcact cgatcgtgct
     2341 tgtgcgcggc ggccgcgtca aggaccttcc tggtgttcga tatcatttgg tgcgcggtgc
     2401 tttagatgcg gtgggtgtag ccgaccgaaa gcaaagtcgt tcgaagtatg gagcaaagcg
     2461 gcctaaatag attctatg
//
LOCUS       DATMTI010000274         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_392660, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000274 DATMTI010000000
VERSION     DATMTI010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392660"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="VN039_04570"
     CDS             complement(<1..1692)
                     /locus_tag="VN039_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWV45293.1"
                     /translation="MHLEDYAFIGDTHTGALVGRNGSIDWLCLPRFDSNACFAALLGT
                     EQNGRWMIAPADQEYRVTRRYRPDTLILETDFTTKDGTVRLIDCMPVRQDHPTIVRLV
                     TGIHGSVRMHMQLILRFDYGSVVPWITRHADGLHAIAGPDSVYIATGAPTHGKQSTRV
                     SDFVVQANQQVSFNLIWHSSHTPVPSAPDAAKAIDETAQWWRQWTSRCHPEGEHREAV
                     IRSLITLKALIYAPTGGIVAAPTTSLPECLGGVRNWDYRYCWIRDATYTLYALLTAGY
                     KEEASNWRDWLIRTIAGDPSQLQIMYGIGGERRLTELELPWLSGYEGSSPVRTGNAAW
                     HQLQLDVFGEVMDTLHQARRTGMETNERGWDIQTTMMEYLESKWKEPDEGIWEIRGPR
                     RHFTHSKVMAWVAMDRMVQDIEGFHLPGDAARWKQVREQIHREVCDKGFDHSRNTFTQ
                     YYGSRQLDAGALMIPLVGFLPPNDPRVQGTVTAIEKELCHDGLIMRYSHQGSQNVDGL
                     PSGEGTFLACSFWLANTYVLQNRRNEAEQLFQRLLALRNDLGLLSEEYDTGAKRLV"
     gene            complement(1890..2387)
                     /locus_tag="VN039_04575"
     CDS             complement(1890..2387)
                     /locus_tag="VN039_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45294.1"
                     /translation="MNTLWRKFIRIVVSVPHYAVMALLWPLAWGGCLLSVWLAYRTAE
                     FIYAHVFQITHEAFRDLLSALLHSIELLLLVPLPGIVGIVAYRNLRLYVNPIAKQRPS
                     IETELEMAKRLVLGTLVSVAGTRMLIQLLEEKTELNLYISGGLLIVSLTFYTVFALGK
                     GRGGH"
     gene            complement(2384..2620)
                     /locus_tag="VN039_04580"
     CDS             complement(2384..2620)
                     /locus_tag="VN039_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45295.1"
                     /translation="MKDRNSEKSFLLSRRSFPSLTLLPGWPFMEPSAPQHLRIGQAEA
                     HLNFERGPSGQVAVHVLQIDGHLEVAVEGFEHER"
     gene            complement(2676..>2929)
                     /locus_tag="VN039_04585"
     CDS             complement(2676..>2929)
                     /locus_tag="VN039_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWV45296.1"
                     /translation="AAGGTDVWSDHRTSHLLGCCRMGDDPADSVVNADCRAHDVPNLF
                     ICDGSVFPNSTGVNPSLTIQALAARTADRICDMARRGEL"
BASE COUNT          652 a          813 c          830 g          634 t
ORIGIN      
        1 caccaaccgt ttcgcacccg tatcgtattc ttcggacaga agcccgagat cgttccgaag
       61 tgccaggagt cgctgaaaca gctgctctgc ttcgttgcga cgattctgga gcacataggt
      121 gttggccaac caaaatgagc aggcgaggaa cgtgccttcg cctgacggca gcccatcaac
      181 attttgagaa ccctggtggg aataacgcat gatcaatccg tcgtggcaca attccttctc
      241 gatcgccgtc actgtccctt ggacacgtgg atcattagga gggagaaaac ccactaacgg
      301 gatcatgagc gctccggcgt caagttgccg cgaaccgtag tattgcgtga acgtgttccg
      361 tgagtggtca aatcccttgt cgcatacttc ccgatgaatc tgctctcgca cctgtttcca
      421 acgagcggcg tcgccgggaa gatgaaaccc ctcgatgtcc tggaccatgc ggtccatcgc
      481 gacccaggcc atgacttttg aatgggtgaa atgccgccga ggaccgcgga tctcccagat
      541 gccttcgtcg ggctccttcc atttggattc aaggtattcc atcatcgtgg tctggatgtc
      601 ccagccacgt tcgttggttt ccatgccggt ccggcgtgcc tgatgaaggg tatccatgac
      661 ttctccaaag acgtcaagtt gaagctgatg ccaagccgcg tttcccgtac gaaccggtga
      721 tgatccttcg tagcccgaca gccaagggag ctccagttcg gtcagccgcc gttccccccc
      781 aattccgtac atgatttgca gttggctcgg atcgccggcg atcgttcgaa tgagccaatc
      841 tctccagttt gatgcctcct ctttgtagcc ggcggtcaac aaggcgtaca gggtataggt
      901 ggcgtcacgg atccaacaat agcgatagtc ccagttgcgg acgcctccga gacattcggg
      961 gagggatgtg gtgggtgcag ccacgatgcc gccggtcgga gcgtagatca aggctttgag
     1021 ggtaatcagc gatcgaatca ccgcttctcg gtgctctcct tcagggtgac atctggaagt
     1081 ccactgccgc caccattgcg cggtttcatc gatcgccttg gcggcatccg gtgcggaagg
     1141 caccggtgtg tgggaggaat gccatatcaa gttgaaagag acctgttggt ttgcttgaac
     1201 gacgaaatca gacaccctcg tcgattgctt cccatgagtc ggcgcgccgg tggcgatgta
     1261 gacgctatcg ggaccagcga tcgcatggag gccgtcggca tgcctcgtga tccatggaac
     1321 gacggaacca tagtcaaatc gaagaatcag ctgcatatgc atccgaacgg aaccatgaat
     1381 gcccgtcaca aggcgcacga tcgtcggatg atcttgccga acaggcatac agtcgataag
     1441 acgcacggtt ccgtccttcg tcgtgaagtc cgtctccagt atgagggtat ccggtctgta
     1501 gcgtcgcgtg acacgatact cttgatcagc aggagcgatc atccaacgtc cgttctgctc
     1561 agtaccgagt agggccgcga aacaggcgtt ggaatcgaaa cgcggcaggc ataaccaatc
     1621 gatggagcca tttcggccta ctaacgctcc cgtatgtgta tcgccgatga aggcgtagtc
     1681 ttcgagatgc atctatttca ccagccggca agttgagcga tggaaatggg tctcgaaaag
     1741 gtggtgcaag cgaaatgcca tgagagagga cctgaaaaat attcatggtt atctaatctc
     1801 gattgggaca atttgacatc atcatgacag ttgttcccga gttcatgtat ttttgagtgt
     1861 tcgtctagta gcgatgccat gtatgtcagt taatgtcctc cccgtccctt gccgagtgcg
     1921 aaaaccgtat agaatgtcag cgacacgatc aaaagaccgc cgctgatgta cagattcagt
     1981 tccgtctttt cctcgagcag ttggataagc atccgtgtac cggccactga gaccagcgtg
     2041 ccgagcacca accgcttcgc catttcgagt tctgtctcga tcgacggccg ttgcttggca
     2101 atcggattga cgtagaggcg gagattacga taggcgacga tgccgacgat gccggggagg
     2161 gggaccagca ataggagttc gattgaatgc aacagggcgg acagaaggtc tcgaaacgct
     2221 tcatgagtga tttgaaaaac gtgggcgtaa atgaattcgg cggttcggta cgccagccag
     2281 acggacaaca gacaccctcc ccacgccaag ggccagagca gcgccatgac tgcatagtgc
     2341 gggactgaga ccacgatgcg gatgaatttc cgccacagtg tgttcacctc tcgtgctcga
     2401 atccttcaac ggccacttcc agatgaccgt ctatttgtag aacgtgcacc gccacctgtc
     2461 cgctcgggcc acgctcaaag ttgagatgcg cttcggcctg gcctatgcgc aagtgctgcg
     2521 gagcgctcgg ttccatgaat ggccaccccg gcaacagggt gagcgaggga aagcttctgc
     2581 gggacagaag aaaggatttc tcggagtttc ggtccttcat gcaatctccg gtcttcgttc
     2641 tctcacgctc gatgcaaacc cacggtgcga acgagctaca actccccgcg cctggccatg
     2701 tcgcaaatac gatcggccgt acgggcggcc agcgcctgga tcgtcaatga cggattgacg
     2761 cccgtcgaat tcggaaatac cgatccgtca caaatgaaca gattgggaac gtcatgggct
     2821 cggcaatcgg cgttgacgac ggaatcggcc ggatcgtcgc ccattcggca acagccgagc
     2881 aggtgcgacg tgcgatgatc cgaccagacg tctgtgccgc cggctgcct
//
LOCUS       DATMTI010000275         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_10665, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000275 DATMTI010000000
VERSION     DATMTI010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10665"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(470..1663)
                     /locus_tag="VN039_04590"
     CDS             complement(470..1663)
                     /locus_tag="VN039_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45297.1"
                     /translation="MNLKTMDFVQSQAQLRALTNELNLTAQRERTRLAGNLHDYLAQL
                     LIVMRMKLRQAIPLVPTENATALLKDADQALTQALDYTRSLVSELAPPTPDEFELLQS
                     LEWLASQMRQHGFTVEIQPAAEALSLPEDQAILLFQSVRELLSNVLKHAHTDEATVSV
                     MITPNSELHVTVEDRGNGFDAAALELSSADSTRFGLFSLKERMATMRGRVSIDSALNR
                     GTKVSLIMPYWPVQPRDESTEHSVPNAESSSPCLESTTPLIHPKLSVQDLARHDKPRI
                     RVLLADDHAIVRQGLRSILESYPDIDIIGEVADGKESLDSVERVRPSVIVMDLNMPRM
                     NGIDATAIIKSRYPEIIVLGLSVNAGDDNQAAMIQAGASALLPKEAAVDQLYEMIHDA
                     VATRS"
     gene            complement(1775..>2432)
                     /locus_tag="VN039_04595"
     CDS             complement(1775..>2432)
                     /locus_tag="VN039_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HWV45298.1"
                     /translation="QATRLAHRRLERQVDSYRPNFDVHNYRRLLQGFWGFYQPLERKL
                     AVLADQNLPSSYSQRRRKAPRLKQDLLSLGVTDTDIAALPLCGRLPAIPTLPQVFGVL
                     YVIDGAALGERIVAIHLGGDKLGISPSQGGSFLSSLSASYDPLLDPWWQDFIAMAETM
                     EDPDLQDLVIRSAMDTLECFETWLDHQKQSPLTNSMNSMRWSSQWEFELFRTDVEKVA
                     "
BASE COUNT          518 a          651 c          670 g          593 t
ORIGIN      
        1 aacctccatt gcctgatcct cagccccctt cacctcccat tcgtccaccg attccacaag
       61 ttttcccgct gcggaatatt cctccaacat ggatggaagc cgaatccgcc attcatgtgg
      121 tcagtcgagt cagaaggcga gaagagcatc cttgtatcgt gaagagccgt ccttacctac
      181 tcacataaag gggatacatc atgcgttatt tcatcctctg gctattgggt gtgccggtga
      241 gcctgcttgt tctgttgtat tttttgggga ttctataaga acatcgtcgc gtcataagct
      301 tctgcaccga caacgacaaa ataaatatcg tctccctccc tatctaacta ctaggtatgc
      361 ccctagccta catcattcat ggagcacttc tgctgccatt gtggcggcat gtcggcgggc
      421 ggactcgccg ctcgggtggt actcgtctgc atcgccccat acaagcgagt catgatcttg
      481 tcgcgacggc gtcatgaatc atttcgtaca attgatcgac tgcggcttcc ttcggcaaca
      541 gggcggacgc gccggcttga atcatggccg cttgattatc atcaccggcg ttcacagaca
      601 accccaggac gatgatttcg gggtagcggg atttgatgat tgccgtagca tcgatgccgt
      661 tcatcctcgg catattgaga tccatgacga tgactgaagg ccgcacgcgt tcgacggagt
      721 cgagcgattc ttttccatcg gcgacttcac ctatgatgtc gatatcggga tagctttcca
      781 gaatgctgcg caatccttgt cgcacgatcg cgtgatcgtc cgccaataag actcgaatgc
      841 gcggtttatc gtggcgtgcc aagtcctgca cgctgagttt cggatggatg agaggtgttg
      901 tggattcgag acaaggactc gatgactcgg cgttcggcac ggagtgttcg gtgctttcgt
      961 ccctcggctg taccggccaa tagggcatga ttaaagagac ctttgtgcct cgattgagag
     1021 cggagtcgat ggaaactcgc cccctcatgg ttgccatacg ttccttcagg ctaaagagac
     1081 cgaagcgagt cgagtcggcg cttgacagct ccagtgccgc agcgtcgaat ccgttgcccc
     1141 tgtcctccac cgtgacgtgc agttcactgt tcggggtgat catcacagac accgtcgcct
     1201 cgtccgtgtg ggcgtgtttg agcacgttgg agaggagttc gcgcacggat tgaaacagca
     1261 agatagcctg atcttccggc aaggagagcg cttcggcggc aggttgtatc tccacagtaa
     1321 agccatgttg tcgcatctga gacgccaacc attccaacga ctgcaagagc tcgaattcat
     1381 ccggcgtcgg cggagccaat tcgctgacta atgatcgggt atagtcaaga gcttgtgtga
     1441 gcgcttgatc ggcgtccttc agaagcgctg tcgcgttctc agtcggcaca agcggtattg
     1501 cttgacggag cttcatgcgc atgacgatga gcagttgtgc caaataatca tgcagatttc
     1561 ctgcgaggcg tgtccgctca cgttgcgcag tcagattgag ctcgttggtc agtgctctca
     1621 actgtgcctg agattgcaca aagtccatgg tcttgaggtt cacgacctat cccttctaac
     1681 ccacatttcc cgacgatcgg gccgcacgat ccggtattca gggtcgttag gaaccgtctc
     1741 ctgagggtgt ctgtcgatcg ttcgatggcg gcgattacgc cactttttcg acatcagtac
     1801 ggaatagttc gaactcccat tgggaactcc atcgcatcga attcatagag ttcgtaaggg
     1861 gagattgctt ttggtggtcg agccatgtct cgaagcactc taaggtatcc atggctgatc
     1921 ggataaccaa gtcctggaga tccggatctt ccatcgtctc agccatagcg atgaagtctt
     1981 gccaccaagg atcgaggaga gggtcatagg atgcgcttaa actggagagg aatgatcccc
     2041 cttgcgatgg actgatccct agtttgtctc ctcctaaatg tatggccacg attcgttcac
     2101 ccagggcagc tccgtcgatg acgtacagta cgccgaaaac ctgtggaagg gttggaatcg
     2161 ccggcagacg tccgcaaagc ggcagcgctg caatgtcagt gtcggttacg cccagcgaca
     2221 gcaggtcttg cttcaaccgg ggggctttgc gccgtcgttg tgaatacgat gaaggaaggt
     2281 tctggtccgc gaggacggcg agtttacgct ccagcggttg ataaaacccc caaaagcctt
     2341 ggagcagccg tcgataattg tggacatcga agttcggacg atagctatcc acttgacgtt
     2401 caagtcgtcg atgggccaag cgggtagcct gg
//
LOCUS       DATMTI010000276         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_817317, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000276 DATMTI010000000
VERSION     DATMTI010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817317"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1152)
                     /locus_tag="VN039_04600"
     CDS             complement(313..1152)
                     /locus_tag="VN039_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HWV45299.1"
                     /translation="MTPVEPITGPRSRHPLEERSACHAPQPFVEINRLSLYYGEKPAF
                     QDVTLSINKGCMTALVGPSGCGKTSFLTCLNRLTDLIADCRVSGRITIDALDVLAPDI
                     DVIRLRRRIGMIFQKPNPFPLTIRKNLEFPLREHGLRDREQITQTMETTLRDVGLWDE
                     VKDRLDSPALALSGGQQQRLCIARALALLPEVLLMDEPCSALDPLSSGVVEDLIVGLR
                     GRYTVLIVTHNLAQARRIADYTALFWVQNGAGRLIEAGTAKQIFENPRDPLTAAYISG
                     MRG"
     gene            complement(1149..2012)
                     /gene="pstA"
                     /locus_tag="VN039_04605"
     CDS             complement(1149..2012)
                     /gene="pstA"
                     /locus_tag="VN039_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWV45300.1"
                     /translation="MAKTMRRPQNPSEWLALILVWGTAGLVTAVFCWLLGDIVWHGLS
                     HVSWTFLTAPPENAGRRGGIGPILVSTSLILGVCLAVSLPIGIGTAVLLAEFTSDHSL
                     FGRVTRRSLDVLAGVPSIVFGLFGNAFFCKTLGLGFSILSGGLTLACMVLPILIRSTE
                     EGFRAVPASYRLSASALGLSRTTTLVHLLLPTAVPGLLVGLVLGVGRAIAETAALIFT
                     SGYVDRMPESLLDSGRALSIHIFDLSMNVSGGDANAYASALVLVIMLLVINGTASWLA
                     TYGLHRKVVTV"
     gene            complement(2005..>2666)
                     /gene="pstC"
                     /locus_tag="VN039_04610"
     CDS             complement(2005..>2666)
                     /gene="pstC"
                     /locus_tag="VN039_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWV45301.1"
                     /translation="ILWGTLFAMTGSVLIATPLGILSAVFCHYYAPLALALPYRRMIE
                     LLAGIPSVVYGFWGLVVLVPLIAEIQPPGPSLLAGILILTIMILPTIALMADASLANV
                     PQQYIRGAAALGLPRWATIRSVVFPAAKSGLFTGVILETGRAIGETMAILMVCGNVVQ
                     IPSSIFDPIRTLTANIALEMAYALGDHRAALFVSGLVLMTIIVALVLAAEWISRGRVY
                     G"
BASE COUNT          642 a          736 c          784 g          504 t
ORIGIN      
        1 ctgacggcag gagatttttc aacctaccgg gaggaaacat gtccttgaat tgcgattggg
       61 cctgcttgaa ggcctcttca agcggatcag actttttgcc ccacggatct tttgggtccc
      121 agaccattca gcggtatgta caatccaata ggtgacagaa aatgagaaga cacattagca
      181 gagtgcggta tgggtaacaa gatctggatg gcccttgata gaaaaagatt gggatggatg
      241 agaagtcata tgccttttct cgtgacaagc ctgttccagt tcagatccgt catgcatcag
      301 gcagcgtgcc cattatccgc gcatcccgct gatatatgca gcggtcagtg gatcacgagg
      361 attctcgaat atctgttttg cggttccggc ctcaatcaac cgtccggctc cgttttggac
      421 ccagaaaaga gcggtataat ccgcgatgcg tcgggcctga gctaaattgt gtgtaacgat
      481 caacacggtg tagcggcctc gcaggccgac aataaggtct tcgaccacgc cgctggagag
      541 cggatcgagg gcgctgcatg gttcatccat gagcagcacc tccggcaaaa gagccagggc
      601 cctggcgata cagaggcgct gttgctggcc cccggatagg gccagagccg gcgagtccaa
      661 ccgatccttg acctcgtccc agaggccgac atctcgcaag gtggtctcca tcgtctgggt
      721 aatctgttct cgatctcgta gcccatgctc gcgtagtgga aactccaggt tttttcggat
      781 cgtcaacgga aagggattcg gtttctgaaa aatcatgccg atgcggcggc gaagccggat
      841 cacatcgata tctggtgcca acacatcgag agcgtcgatc gtgatccgtc ccgagacacg
      901 acagtccgcg atcagatcgg tgaggcggtt caggcaggtg aggaagctgg tcttcccgca
      961 tccggacggc cccacaagag ccgtcatgca tcccttattg atcgacaagg tcacgtcctg
     1021 aaacgccggc ttttccccat agtacaagct gagtcgattg atctcaacga agggttgagg
     1081 cgcatggcaa gcagatcgtt cttcgagtgg atgtcgagag cgcggtcctg tgatgggttc
     1141 gaccggcgtc atactgtcac gacctttcgg tggagcccat atgttgccag ccaggaggcg
     1201 gtaccgttga tgacgagtag catgatgacc aaaaccagcg ctgaggcata ggcattcgcg
     1261 tctccgccgg acacgttcat ggagagatca aagatgtgga tagacaatgc gcgaccggaa
     1321 tccagcagcg actccggcat ccgatccaca tagccgcttg tgaagatgag cgcagctgtc
     1381 tcggcaatcg cacggccgac tccgaggact agaccaacca agagaccggg tactgccgtc
     1441 ggtaacaaga ggtgaacaag ggtcgtggtg cgggacagtc cgagtgccga ggcggaaagc
     1501 cggtaactag ccggcacggc gcgaaaccct tcctctgttg atcggatcaa gatcggcaac
     1561 accatgcagg ccagagtcag cccgccggaa aggattgaaa agccgagccc cagcgtcttg
     1621 caaaagaacg cgttgccgaa caggccgaag acgatcgacg gcacaccggc tagaacatca
     1681 aggctgcgac gagtcacccg tccgaacagg ctgtgatccg acgtaaattc agcgaggagg
     1741 acggcggtgc cgataccaat ggggagcgat accgcgagac agactcccag aatcaaggat
     1801 gtcgaaacca ggatagggcc gatcccgcct cggcgaccgg cattttccgg cggggctgtc
     1861 agaaatgtcc aagagacgtg gctcagtcca tgccaaacga tgtcccccaa cagccagcag
     1921 aatacggcgg tcacgagccc tgccgttccc cagacgagaa tcaaagcaag ccattcactg
     1981 ggattctgtg gccgcctcat cgtcttagcc atacactctc ccgcgactaa tccattcagc
     2041 ggctagaacg agcgccacga ttatggtcat caagacaagt ccactgacga acaacgcagc
     2101 gcgatggtct ccgagcgcat aggccatttc cagggcgata ttggcggtca gcgtccggat
     2161 cgggtcgaag atgctagaag ggatctggac tacgttccca cagaccatca gaatcgccat
     2221 cgtttccccg atggctcggc cggtttccag gatcaccccg gtgaacaatc ccgattttgc
     2281 cgctggaaac acaacgcttc ggattgtcgc ccatcgcggc agtcccaatg cagcggcccc
     2341 gcgtatgtat tgttgaggta cgttggcaag actggcatcg gccattaacg cgatggtggg
     2401 gaggatcatg atcgtgagga tcaggatgcc cgctaagaga ctgggccctg gcggttgtat
     2461 ctcggcaatc agcggaacca gcacgacgag tccccagaat ccgtagacca cggaaggaat
     2521 gcctgcgagc aactcgatca tgcgccgata ggggagggcg agagcgagtg gcgcatagta
     2581 atggcagaag acggccgaga gaatgcctaa gggtgtggcg atcaacacag aaccggtcat
     2641 ggcgaacagc gttccccaca atatcg
//
LOCUS       DATMTI010000277         1657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_817338, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000277 DATMTI010000000
VERSION     DATMTI010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817338"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VN039_04615"
     CDS             <1..649
                     /locus_tag="VN039_04615"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HWV45302.1"
                     /translation="ARLGINPKEHDIRFIQDDWESPTLGAWGLGWEVRLDGMEITQFT
                     YFQEIGGIELAPITGEITYGTERIAMYLQEVNNVFDLAWNNTVSYRDIHHETEVQGSR
                     YNFEEADVAMLMQGFQANEAECKRLLAQTDKRLTLPAYDYCIKSSHLFNLLDARGAIS
                     VAERTGYIARVRALARQCAERYIEERAAMGHPLMSRENRAVKSGRPRLKAGTGRT"
     gene            656..>1657
                     /gene="glyS"
                     /locus_tag="VN039_04620"
     CDS             656..>1657
                     /gene="glyS"
                     /locus_tag="VN039_04620"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWV45303.1"
                     /translation="MATQKKAGRRSAVPRKGRGTSAPTVVELLLEIGVEELPYQFIVP
                     ALASLKESAERLFNDQRLTFQSVRTMGTPRRLTIVVDGLAARQTSMTKEAMGPSKAVA
                     FDQAGQPTRAALGFAVGQGVAVQDLQVRQTPKGEYVFAVKREEDRPAAAVLIDMLPQL
                     VSGLAFPKAMKWNETGVRFARPVRWMVALLGGSVLPIEAAGIKASNQTKGHRVLAGGK
                     WVSVRDAASYISTLERQGVIADPQRRRKLIEEQIAAICHKTGLQLNEDESLLDQAVYS
                     TEQPISVIGSFRDAYLDVPEEILITSMKEHQGFFSLRQQNTGKLAAHFIAVANNRIKD
                     "
BASE COUNT          378 a          443 c          509 g          327 t
ORIGIN      
        1 agcccggctg gggatcaacc ccaaggagca cgatatccgt ttcattcaag acgattggga
       61 gtcgccgacg ctgggtgcgt gggggcttgg gtgggaagtg agactggatg ggatggagat
      121 cacccagttt acctactttc aggagatcgg cgggattgaa ctggctccga tcaccggcga
      181 aatcacctat ggcacggagc gcatcgccat gtatctgcaa gaggtgaaca acgtctttga
      241 tttggcatgg aacaacacgg tctcatatag ggatattcat cacgaaaccg aggtgcaagg
      301 gtcgcgctac aatttcgagg aagcggacgt cgcgatgctc atgcaaggct ttcaggcgaa
      361 tgaagcggaa tgcaaacgac tgctcgcgca gaccgacaag cgcctgacat tgccggccta
      421 tgactactgc atcaaatcat cccatttgtt caatctcctc gatgctcgcg gggcgatcag
      481 tgtcgcggaa cggactggtt acatcgcgcg agtgcgggca ctggccaggc aatgcgccga
      541 gcgttatatc gaggagcggg ctgcgatggg acatccgctg atgagtcgcg agaatagagc
      601 cgtgaagtcg ggcaggcctc gcttgaaggc cggaacaggc cgaacgtaac cagttatggc
      661 gactcaaaag aaagcaggac gccggagcgc tgtgccgagg aaaggcagag ggacctccgc
      721 acctaccgtg gtcgagttgc tgctggaaat cggcgtggaa gaactgccct atcagttcat
      781 tgttcccgct ctggcatcac tcaaggaatc agctgaacgt ctcttcaacg atcagcggct
      841 cacttttcaa tctgtacgca ccatgggcac gccgcgccga ttgacgatag tggtcgatgg
      901 cctggcggca agacagacct ccatgacgaa agaggcgatg ggaccgtcga aggccgtggc
      961 gttcgaccag gccggccagc cgaccagagc tgccttaggg tttgccgtag gccaaggtgt
     1021 cgccgttcag gatcttcaag ttcgtcagac ccccaaaggc gagtatgttt ttgcggtgaa
     1081 acgtgaagaa gaccgtccgg ctgccgcggt gctgatcgat atgctgccgc aactggtgtc
     1141 gggcctcgcg tttcccaagg cgatgaaatg gaatgaaacc ggcgtacgct tcgcccgtcc
     1201 ggtccgctgg atggtagcgc tcttaggagg ctcggtgctt cctatcgaag cggcgggcat
     1261 caaagcatcg aatcagacga aaggccatcg ggtcttggcc ggagggaagt gggtatctgt
     1321 tcgagatgcc gcctcctata tcagtacatt ggaacgtcag ggggtcattg cggacccgca
     1381 gcgccgccgc aaactgatcg aagaacagat cgccgccatt tgccataaga ccgggcttca
     1441 gttgaacgaa gacgaatcct tgctcgacca ggcggtctac tcgacggagc agcccatctc
     1501 ggtcatcggg tcgttcaggg acgcctatct ggatgtaccg gaggagattc tgattacctc
     1561 gatgaaggaa caccaaggat ttttttcctt gcgccagcag aataccggca aactggctgc
     1621 ccatttcatt gccgtggcca acaaccgtat caaggac
//
LOCUS       DATMTI010000278         6539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_689371, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000278 DATMTI010000000
VERSION     DATMTI010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689371"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..293
                     /locus_tag="VN039_04625"
     CDS             159..293
                     /locus_tag="VN039_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45304.1"
                     /translation="MATSVKQTYSPRVNTSETRKDGRIAKFTVWQTGVTGSTQRIVVP
                     "
     gene            293..511
                     /locus_tag="VN039_04630"
     CDS             293..511
                     /locus_tag="VN039_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45305.1"
                     /translation="MRTIIRTSDNGNGKILAKGGGKQRTIASDPAKSNNWNHAIAAGT
                     LASVLGLTWHPGIGHDSNESGTKHGFEF"
     gene            813..1094
                     /locus_tag="VN039_04635"
     CDS             813..1094
                     /locus_tag="VN039_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45306.1"
                     /translation="MDITKVVFSDHFKRQFRTKGFTIAQIREAIENPYKVTKVSRYPG
                     QLRYCGGGVAVVMRDNHAITVYLDGVVTPLRDDQKNDPAALASRRLNEV"
     gene            2122..2328
                     /locus_tag="VN039_04640"
     CDS             2122..2328
                     /locus_tag="VN039_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45307.1"
                     /translation="MSWISDAHQEWHHANLDRPWDCPLDCGAGEPSYAEEQYENALYY
                     EELELARQEEYDYSVWVPGMEPVV"
     gene            2325..2516
                     /locus_tag="VN039_04645"
     CDS             2325..2516
                     /locus_tag="VN039_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45308.1"
                     /translation="MTEQEFIEKMESEGVDYAFTGYGLSEADLDDSVSPEFRKAVVDT
                     RIAYERASHFAERIYALAP"
     gene            2993..3124
                     /locus_tag="VN039_04650"
     CDS             2993..3124
                     /locus_tag="VN039_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45309.1"
                     /translation="MATVMAGIVLGMILAFFLALWISGVFIIIAPLAILFKFFFPNR"
     gene            3187..3498
                     /locus_tag="VN039_04655"
     CDS             3187..3498
                     /locus_tag="VN039_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45310.1"
                     /translation="MAKSSRVTRPTAKDVRAFAAAHPERFSTLSERSQHTLVSRGQLA
                     QDVIDTYNKGVKPERQYVRGQSGAVKGERMALRHSLIEKGLAGKRGPLSKAAQESLVS
                     K"
     gene            3728..4297
                     /locus_tag="VN039_04660"
     CDS             3728..4297
                     /locus_tag="VN039_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45311.1"
                     /translation="MTTDIDYAVNIMNTYKRATADEIADGTGWYARARDLALELCPND
                     VWRGAGVLAALSPLKSWPLNVELAREAFATGEARGHFPAQCDKANRIMAGADVLSTLN
                     GKKTVSFAIAIATGGRGEVATIDGHAHDAAIGESYAWETRRINKSTFDEMDAAYREVA
                     DYLGLAVNDLQAIVWVVWKREKKASRRAS"
     gene            4803..4982
                     /locus_tag="VN039_04665"
     CDS             4803..4982
                     /locus_tag="VN039_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45312.1"
                     /translation="MNETLPEPEKNYAEGMTITAMWDELNDMGVDGNLIVKYWAVGNI
                     SDLYNRVIQEALNHA"
     gene            4975..5154
                     /locus_tag="VN039_04670"
     CDS             4975..5154
                     /locus_tag="VN039_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45313.1"
                     /translation="MRSLIIIEVEHGESLDPLNDFAYEIQTISHESDTLEGLTMLDYT
                     FRVDLPQCFTLDKGE"
     gene            5157..5342
                     /locus_tag="VN039_04675"
     CDS             5157..5342
                     /locus_tag="VN039_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45314.1"
                     /translation="MYFHTDSTSAREFIENVARLATENEQSLRIDVDAYGNLSIKRGE
                     GVWSAPIYSTPDPYRDQ"
     gene            5474..6466
                     /locus_tag="VN039_04680"
     CDS             5474..6466
                     /locus_tag="VN039_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HWV45315.1"
                     /translation="MTDNIANDGKRDLFASFREVAWHRLGTVFQSEVTSSAQMLDLAG
                     LSNWNVREENISVVGSPNANFAVPAKAIVATMPDGDRVLGVTGERYKIVQNEEAFSFL
                     QSLHDGARWETAGAIKDGRVVFGSMAFSRDFVIDESGVADKVESYLLVYTSHDGSTGV
                     AGGVTPVRVVCQNTLNAAIPGVKQSFKIRHTTNAQERMAAESVLWRGAYTYMDAFETE
                     ARELYATKATTKDYLSIVDDIFPKPEKDVKGALSKWENRRGLFVQAWNGKPNAGIKGT
                     AWGVFNALTEANQWGRSTRKSGNGAENFAAAGAGFDIPTNQFRATALARAKALV"
BASE COUNT         1449 a         1818 c         1818 g         1454 t
ORIGIN      
        1 acggctccta cgaggagtga gcgtcagtgc ccgaaagggc actgaccccc gggggtcgaa
       61 aactgtctca tattatgaga tacaggtagc ggcggtagtg aatataggct atctttgtct
      121 tattggagga accgcctccc aagcagagag agaatcccat ggccaccagc gtcaagcaga
      181 cctactcacc tcgcgtcaac acttccgaga cgcgcaagga cggacgcatc gccaagttca
      241 cggtgtggca gaccggcgtg accggttcga ctcagcgtat cgtggtgccg tgatgcgcac
      301 catcatccgc acttccgaca atggcaatgg caagattctg gccaagggcg gaggcaagca
      361 acgtaccatt gcctccgacc ccgccaagtc gaacaactgg aaccacgcca tcgccgctgg
      421 cacgctggct tccgtgctcg gtctcacgtg gcaccccggc atcggccacg actcgaacga
      481 gtccgggacc aaacacggtt tcgagttctg acccgtcgcc cttcggggcg acccccgggg
      541 gttccaatgc ttgacaccat tacatttatc gcttacgatt gacgcatgac gacaatcaac
      601 gaccaaatcg aaatcgctca gcgcctcaat gacggagcct tcttctcgaa ccacccaagc
      661 catgacttca acggctttga tggtcgctgc ttcttctgcg actgccgccc gttcggacgc
      721 cacgctccca atccttgtcc ggtcgctcac tgagtgaccc ccgggggttc ccgggggttg
      781 acacccgcat taagacggga tacgatttcg ccatggacat caccaaggta gttttctccg
      841 accacttcaa gcgtcagttt cgtacgaagg gcttcacgat tgctcagata cgtgaggcta
      901 tcgagaatcc gtacaaggtg acgaaggtta gccggtaccc cggacaactt cggtactgtg
      961 gtggaggcgt ggccgttgtt atgcgcgaca accatgccat cactgtctac cttgatggcg
     1021 tggttactcc tctcagggat gaccagaaga atgacccggc agcacttgca tctcgccgtt
     1081 tgaacgaggt ttgaagtcag cgccccggcg ctgacccccg ggggttcggt accggatagt
     1141 tccttttacg atagttcttg acatcgccag agttccatga gaagatttgg tcatgaccaa
     1201 gaaacagcgt atcgcacagg cgaagaagcc tcgcatgtct ggcgagaaca aggaatggat
     1261 gcgtgcccga ctcgaccagt cgaatcgtaa cctccgcgtg ccgtctggca aggcttaccg
     1321 tcgtccgagt cctggaaaga ggtgggagtg atgggagcat tcaatccctg gctctggatg
     1381 gcaggtgcct tcctgtggat tctcatagcc ctcgttgttg tcagcctcgt ctgaggctga
     1441 cccccggggg taccggtcta gaccactctt ttacgatggt tgactcaatc acggggaagt
     1501 gttagagtgg tgccaacaac aaaacagaga cttagagggc tagccacagg gcaacgtgct
     1561 acgcttagag acggcagcga ctaacacagg ttagcgtgta tccgatggtg gttgataagt
     1621 aggcacccct ctatctactt accgggccgg tagtgtaaga ctaaccaatg tgggaatgcc
     1681 tctaccgtcc ggtaacacaa cttggatata gggtttgagc ccctagcata ttgttaaagg
     1741 tcatcacttg caatccctgc gcatgagttt gctatccata ggtcaatcca gtaccgccca
     1801 aaagggtctg cgcccaggcg tctcgacgga gatagcaggc tgccgctgag taggcatcgg
     1861 gctgggttga ccgccccaac ttccctggca catgggaggg cgcggtactg agacaagttc
     1921 ctcagcgtga cagcgcacaa tatgtgcaaa tggttcagtg tcatcggtgg taaacgagta
     1981 acgtgtgttg aggtgtaaag attcaaaggt ggccgcgccg taatggccaa cacacgccat
     2041 gtgtcccacg ttgactatgc tgcgcctacc cccgggggtc aggggttgac aagtcccttt
     2101 tacgatagta cgatatgttt catgagttgg atttcagacg cacaccagga atggcatcac
     2161 gcaaaccttg accgtccttg ggattgtcca ttagactgtg gtgctggtga gccttcgtat
     2221 gctgaggagc agtacgagaa tgctctgtac tacgaagaac tcgaactcgc ccgtcaggaa
     2281 gaatacgatt actcggtctg ggtaccggga atggagccag tggtatgaca gagcaagagt
     2341 tcatcgaaaa gatggagtcg gaaggcgttg actacgcttt caccggctac ggtctgtctg
     2401 aggctgacct tgacgattcc gtctcaccgg aatttcgtaa ggcagtcgtg gacactcgta
     2461 tcgcatatga gcgtgcgtct cacttcgctg aacgcatcta cgctctggca ccgtgaggtg
     2521 ccccccgggg ggcattcgac cggaaaaaca cttgacttta cgttacgaaa tgaggtatca
     2581 tcgggatatg aagccgaaag aagcgctagt caaagacgga ttcttgccca agggtagtga
     2641 gaacacgcgc gggcgcatgt ctcgtgatgc cattgccagg tgtgaggagt tggcatccaa
     2701 gggttggcag attgacggat tctcggtcac caagtcttcc gacaattcaa agcctgacac
     2761 tgtggcgagg attaagacag acccaaactc ggtagcagag attcccaacc ctacccgcga
     2821 cgagtcagcc atgaatgcat attctggcac tgtcaaggtc ggcatgcgca cagtttgcaa
     2881 taactgtcga cgtagcctca cctactgcct gtgtgaatct cctcgtgtgt ggcttgacca
     2941 cgagacacaa ggcatagtat acttcaagaa catctaggct taggggtata ctatggcaac
     3001 cgtcatggcc ggaatcgtac tcgggatgat tttggcattc ttccttgcat tgtggataag
     3061 tggtgttttc atcatcattg cgccgcttgc aatattgttc aagtttttct ttccaaaccg
     3121 ttgacaaaca tctagatagt gactagtata gtcactatca accttcgagc aacaaggagt
     3181 ttcatcatgg ccaagtcttc tcgcgttacc cgccccaccg ccaaggacgt ccgtgcgttc
     3241 gcggccgctc atcccgagcg cttctccacc ctctccgagc gctcgcagca cacgctcgtg
     3301 agccgtggcc agttggccca ggacgtcatc gacacctaca acaagggtgt caagcccgag
     3361 cgccagtacg tgcgcggtca gtcgggtgcc gtcaagggtg agcgtatggc gctccgtcac
     3421 tcgctcatcg agaaggggct cgctggcaag cgtggccctc tctccaaggc tgcccaggag
     3481 tctctggtca gcaagtgacc aaagaagggg agaggttgac aagcctctcc cctttctggt
     3541 acactagcaa catccgagaa tgcctcgtga gcggtaccct gtcagtcgtc gggctagggt
     3601 ttgaagagtt tccgccctca cgaggttttt ctctgccccg acccccgggg gtcgggggcg
     3661 gtccacaatg tggaccgctt tacgattacc tgttgacatc accagagaat catgtagaat
     3721 cgtgactatg acgaccgaca ttgactacgc cgtgaacatc atgaacacct acaagcgtgc
     3781 caccgctgac gagattgccg atggtacggg atggtacgcg cgcgcgcgcg acctggcact
     3841 agaattatgc cccaatgatg tatggcgcgg ggcaggtgtt cttgccgcat tgtcaccatt
     3901 gaagtcatgg ccattgaatg tcgagttggc acgtgaggct ttcgccactg gcgaggcacg
     3961 cggccacttc ccggcacagt gtgacaaggc taatcgtatc atggccggtg ccgatgtcct
     4021 gtcaactctg aacgggaaga aaaccgtttc gttcgcaatc gctattgcca cgggtggacg
     4081 aggcgaggtt gccacaatcg acggacatgc tcacgatgcc gccataggag aatcttacgc
     4141 ctgggagaca cggcgtatca acaagtctac ttttgacgag atggacgcag cctaccgtga
     4201 ggttgccgat tatcttggcc tggcagtcaa tgacctacag gctatcgtct gggtagtctg
     4261 gaagcgtgag aagaaggcca gccgtcgtgc ctcctgacgc atctccgctt agtggggcct
     4321 gtatcttcgg ggatacaggc ccttactata ttgacaagga atatgcacaa ctaggtttat
     4381 cggaaggttg gctaatactt gacggatact ttttcaatga acataggata ttgacattct
     4441 actaccggct ccggtcgccg cctcggtgac gacccccggg ggtcatgtta acttttggca
     4501 ggattcctaa cacgttacga tgacatgtta aacccttgac atggcgcggt aggcgagtat
     4561 acttagtttc atgaccaagc cattgagcat gaccttccac gagtcctacg gttcgcttcc
     4621 taccgcgctg ttgaagactt acaggcggta caatgtgagc cccgctgacc atgacctgat
     4681 tcttttcaat gtcggttaca tggtcggaga tacttccatt ccgtggtcac tcgtgctaga
     4741 ctttgttcta gcaaacgtcg aagacggctc attccgtctt cccatctaca tgtgaggctg
     4801 gtatgaacga gacacttccc gagcccgaga agaactatgc cgagggtatg actatcactg
     4861 ccatgtggga tgagttgaat gacatggggg ttgacggtaa cctcattgtc aagtattggg
     4921 cggtaggcaa tatctccgac ctctacaacc gagtcattca ggaggcactg aatcatgcgt
     4981 agtcttatca tcatcgaggt tgagcatggt gaatcactcg acccgctgaa tgacttcgcg
     5041 tatgagattc agacaatctc acacgagtct gacactctcg aaggcttgac tatgcttgac
     5101 tacacttttc gtgtagactt gccccaatgc ttcacactcg acaagggaga atgacaatgt
     5161 acttccacac cgactcgacc agtgcgcgtg agttcatcga gaacgtggca cgtctggcaa
     5221 ccgagaatga gcaaagcctg cgtatcgacg tcgatgcata tggcaatctc tccatcaagc
     5281 gtggagaagg tgtatggtct gcaccgattt actcaactcc tgacccgtac cgcgaccagt
     5341 gaacagtcag cgcttcggcg ctgacccccg gggggcgtct cacgatgtga gacgccttaa
     5401 gattcagact tgacaatctg gggaatcgag aggagaatgg agtcatccag tcaaaccaac
     5461 caaggagata tacatgactg acaacatcgc caatgacggc aagcgtgacc ttttcgcttc
     5521 gttccgtgag gtcgcctggc accgcctcgg cacagtcttc cagtccgagg tcacttcctc
     5581 ggcgcagatg ctcgaccttg ccggtctgag caactggaac gtgcgcgagg agaacatctc
     5641 ggtcgtcggt tctcccaacg ccaatttcgc agtcccggct aaggctatcg ttgccacgat
     5701 gcccgatggc gaccgcgttc tcggcgtgac gggcgagcgc tacaagattg ttcagaatga
     5761 ggaggctttc tcattcttgc agtcgcttca cgatggtgca cgctgggaga ctgccggtgc
     5821 catcaaggat ggccgtgtcg tgttcggctc gatggccttc tcgcgtgact tcgtgattga
     5881 cgagtcgggt gtcgctgaca aggtggagtc atacctcctc gtctacactt cgcacgatgg
     5941 ctcgaccggc gtggccggtg gcgtcactcc tgtccgtgtc gtctgccaga acacgctgaa
     6001 cgctgccatt cctggcgtca agcagtcttt caagattcgt cacacgacga acgcccagga
     6061 acgcatggct gccgagtccg ttctctggcg tggtgcctac acctacatgg atgctttcga
     6121 gaccgaggcg cgtgaactct acgcgaccaa ggcaacgacc aaggactacc tgtccatcgt
     6181 tgacgacatc ttccccaagc ctgagaagga cgtcaagggc gctctctcca agtgggagaa
     6241 ccgtcgcggt ctgttcgtgc aggcttggaa tggcaagccg aacgctggaa tcaagggcac
     6301 cgcttggggc gtgttcaacg ctctgaccga ggccaaccag tggggccggt cgactcgcaa
     6361 gtccggcaac ggtgccgaga acttcgctgc cgctggcgct ggcttcgaca ttccgaccaa
     6421 ccagttccga gccactgccc tggctcgcgc taaggctctg gtctgagtca ccgcaaagcc
     6481 tggcccttcg gggccaggct ttggcatgtc cgcctgaccc ccggggggca ttttgcata
//
LOCUS       DATMTI010000279         5396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_604780, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000279 DATMTI010000000
VERSION     DATMTI010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604780"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VN039_04685"
     CDS             <1..512
                     /locus_tag="VN039_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45316.1"
                     /translation="VERQDGFLSVKFTSKQLEEMMGHVGIIIAAVGDRQAGYLCASSC
                     EFNRPFPLLDAMMRQFDGIDYRGRSLASSRAFIYGPVCIDRSYRGRGLLRGLYDTLQQ
                     EVVGRYDIGVAFVADDNPHSLRAHVDGLGMARVGGFVFSGKPYHILAFHVRSENGNGA
                     ASKPAFEKT"
     gene            509..1099
                     /locus_tag="VN039_04690"
     CDS             509..1099
                     /locus_tag="VN039_04690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWV45317.1"
                     /translation="MSKQRQELWLIRHGETEWSQAKRHTGRTDIPLTPAGERKAAALG
                     QSLAGRRFGLVLCSPLRRASETCRLAGYGPVAEVTDDLLEWDYGVYEGRTTEEIRLEQ
                     PGWSIWTASVTKGESIEQVGHRAQRVIQRALAVDGDVVLFAHAHILRILTACWLGLPP
                     DAGRLFVLRTASISVLGFERETRVVTRWNLLQEEHI"
     gene            1096..1542
                     /locus_tag="VN039_04695"
     CDS             1096..1542
                     /locus_tag="VN039_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV45318.1"
                     /translation="MKAHYLGHVVFYVKSLDQSLGFYRDLLGFKEVGRVFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPKGSRRGLYHIGIKVGDNLDELRRAKQELEQAGVSIDGM
                     SDHTVSQSLYLKDPDGNEVELYVDADESVWKNNPAAVLSPIKPLQL"
     gene            1639..2313
                     /locus_tag="VN039_04700"
     CDS             1639..2313
                     /locus_tag="VN039_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45319.1"
                     /translation="MALTVDDIKKWLGSLEQKRDELRLQAHLLGMEARDEFDKVDRSI
                     DKFRGAVQWVSSTASAAAREAPATIKTGAEKLTRTVSDSITELRAAHADTLDTLKQRA
                     SDLHAEIEKATLSLQETVGSLKDKAGETSADFRRRAEEAKTEFAAKAEDAKTQIAGKL
                     EEMKHKGQEMKERSELLSEGVKGALKGMAEDFSRHLGELHTDLDKRLTALKGKVESSG
                     RSEAKK"
     gene            2534..3289
                     /locus_tag="VN039_04705"
     CDS             2534..3289
                     /locus_tag="VN039_04705"
                     /inference="COORDINATES: protein motif:HMM:NF009385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV45320.1"
                     /translation="MNNHTLKGKVAVIGAGAKNLGGLLSREFAKGGAKIVVHYNSDAT
                     KPNADETVKAIKDVGGDAYAVQSDLTKPANVTALFKSAKDRFGGIDIAVNTVGKVLRK
                     PIVDTTEAEFDSMFDINAKAAYFFIKEAGKNLNNNGKIITIVTSLLAAFTDGYSTYAG
                     GKAPVEHFTRAAAKEFGPRGISVTAVGPGPMDTPFFYGQETPERVAFHKSQGMNGQLT
                     KIEDIVPIVGFLATEGWWVTGQTIFANGGYTTR"
     gene            3384..4154
                     /locus_tag="VN039_04710"
     CDS             3384..4154
                     /locus_tag="VN039_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWV45321.1"
                     /translation="MTRSTSGRQWTPPREKYDGKAHRNSNKVLVVDVGGTNVKILATG
                     KRAPRKISSGPNMTAQDMVEAVRKLAADWSYDAVALGYPGAVRDGRPAAEPKNLGDGW
                     MKFDFEKAFGCPVKVINDAAMQALGSYDGGRMLFLGLGTGLGSALITDGVLVPMELAH
                     LRYRKGRTYEDYVGLRGLKRFGKKKWRRYVMDIVTALKNALQADHVVLGGGNAKRLKR
                     LPKDVRLGDNEHAFTGGFRLWEEAWKDTRAACVVHQGR"
     gene            4459..5382
                     /locus_tag="VN039_04715"
     CDS             4459..5382
                     /locus_tag="VN039_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45322.1"
                     /translation="MRKMMILGLMVLSLSMPVSYALADQGGTHGHGKGTHMKVTGTVS
                     AIQSDLITVKTSWGQIRISSATAPKNLEVGEEVEMQVNENNVVVDVHRAGDPSHSHRF
                     VSGKLAYASTDRKEIKLWTPEGKKKFDVQTGRSQLSGIKEGTPVAVELNEAGKVIDIH
                     RMTVEIELDEHPHTLSGYHLTLDGVVTKIQSGLAFVKTPVGQYTIPMKQAPPDAAVGD
                     KVTLWLNEENLVIDHHGKHKNKPGTHRLITGKLIYAGLTKQEIKLWTPEGEKVFPLDR
                     MEVKTKPIEEGSMITIELDEQGTVIDLRKAE"
BASE COUNT         1298 a         1407 c         1662 g         1029 t
ORIGIN      
        1 aggtggaacg ccaggatggc tttttgtcgg tgaagtttac atctaaacaa ctcgaggaga
       61 tgatgggcca tgtcgggatc atcatagccg ccgtcggaga ccgccaggcc ggttacctct
      121 gcgcctcctc ctgtgaattc aatcgaccgt tccccctgct tgacgccatg atgcggcagt
      181 tcgacggaat cgactatcgc ggccggtcgc ttgcgtcctc tcgtgcattc atttatggac
      241 cggtgtgcat cgatcggtct taccgcggcc ggggacttct ccgcggattg tacgacacac
      301 tgcaacagga agtcgtcggg cggtatgaca tcggtgtagc cttcgtcgcc gacgataatc
      361 cgcattccct tcgcgcccat gtggatggac tcggaatggc tcgagtcgga ggatttgtct
      421 tctccgggaa gccgtatcac attctggcgt ttcatgtgag atccgaaaat ggaaatggag
      481 cggcgagtaa gccggctttc gaaaagacat gagcaagcaa cgccaagaac tatggctgat
      541 ccggcacggt gaaacggaat ggagccaggc caagcggcat acaggccgga ccgatatccc
      601 cctgacgccg gcgggcgagc gcaaggctgc cgcgttgggg cagtctctgg ccggtcggcg
      661 atttggattg gtactctgta gcccgttgcg ccgggcgagc gagacctgcc gtctggccgg
      721 gtatggaccg gttgccgaag tgaccgatga cctgctcgaa tgggactatg gtgtctatga
      781 aggaagaacg acggaggaaa tcaggttgga gcaacctggg tggtcgatct ggacggcttc
      841 agtcaccaag ggcgagtcga tcgagcaggt cggacaccgc gcccaacggg tgatccagcg
      901 ggcgctggcg gttgacggcg acgtggtgtt gtttgcacat gcccatattc tgagaatact
      961 gactgcgtgc tggctcggtc ttccgcccga tgcggggcgg ttgttcgtgc tccgcaccgc
     1021 ctcgataagc gtgctcggct tcgagcgcga aacgcgcgtc gtcactcggt ggaacctcct
     1081 acaggaggag catatatgaa agcacattat ttgggtcacg tggtcttcta cgtgaaaagc
     1141 ctagaccaat cgcttggttt ctaccgagat ctgttgggct tcaaggaagt cgggcgggtc
     1201 ttcaacggag cagccgcggc cctgacatca ggccggacgc atcacgaatt attgttgatc
     1261 caagtcggcg atgctccggg accaccgaag ggatctcgcc gagggcttta tcatatcggc
     1321 attaaggtgg gagacaactt ggatgaactc cgacgcgcca aacaggagct tgaacaggca
     1381 ggcgtctcga tcgacggcat gagtgatcat acggtgagcc agagtctcta tctcaaagat
     1441 cccgacggca atgaagtgga gctctacgtc gatgcggatg agtcggtatg gaaaaacaat
     1501 ccggcagcgg tgctgtcacc gattaagccg ctgcagttat agcgtggtac agatatggga
     1561 caaaagggac acaattgagc acgctctgca atgcctcacc gtgggctcat gaatcgcgct
     1621 aaaaaggagg acaggaacat ggcgttgacg gtcgatgata tcaagaaatg gctgggttcc
     1681 ttggaacaga agcgggatga gctgcggctg caggcccacc tgttggggat ggaggcccgc
     1741 gacgaattcg acaaagtgga ccgatcgatc gacaagtttc gaggtgcggt gcaatgggtg
     1801 tcgtcaaccg catcggccgc ggctcgggaa gcacccgcta cgatcaagac cggtgccgag
     1861 aaactcacca ggactgtctc ggattccatc actgaattac gcgccgcgca cgccgacacc
     1921 ctcgataccc tcaaacaacg ggccagcgat cttcacgcgg agatcgaaaa ggccaccctg
     1981 tcgctgcaag aaacagtcgg atcgttgaaa gacaaggcgg gcgagacgtc ggccgatttt
     2041 cggcgccgag ccgaggaagc caagacggaa ttcgccgcaa aagccgagga cgccaaaacg
     2101 caaattgcag ggaagcttga ggagatgaag cacaaaggcc aggagatgaa ggaacggtcg
     2161 gaactcctgt cggaaggagt gaaaggagca ttgaagggca tggcggaaga cttttcacgt
     2221 catctcggcg aactccatac cgatctggac aagcggctga ccgcactcaa gggcaaggtc
     2281 gaatcgtccg ggcggtccga agccaagaaa taggatgccg catcgggtca agaacatagc
     2341 tgatcaagag aaagcgtggc ggtggcagac gaaatggcgc gcgctgtgtc gggccatgat
     2401 ctcgcggtca gttgttcaat ccagatgttt acaatcttca acatgggcta ttaaacacaa
     2461 tatggaagct catctgaacg cgagcacgct ctgactcgtt cctgaactga ccatcaatgg
     2521 aaggagaaac atcatgaaca atcataccct gaaagggaaa gtcgctgtca ttggggccgg
     2581 cgccaagaat ttgggcggcc tcctcagccg cgagttcgcc aagggcggtg ccaagatcgt
     2641 ggtgcactac aacagcgacg ccaccaagcc gaatgccgac gaaacggtga aagccatcaa
     2701 agacgtcggt ggtgatgcct atgccgtcca gagcgacttg accaagcctg ccaatgtcac
     2761 tgcgttgttc aaatccgcca aggaccgctt cggcggtatc gacatcgccg tcaataccgt
     2821 cggcaaggtg ctgcgcaaac ccatcgtcga caccaccgag gcagagttcg actcgatgtt
     2881 cgacatcaac gctaaagcag cgtatttctt catcaaggaa gccggaaaaa atctcaacaa
     2941 caacgggaag atcatcacca tcgtgacctc gctgctcgcg gccttcaccg acggctactc
     3001 gacttatgcc ggcggcaagg ccccggtaga acatttcacc cgcgcagcag ccaaggagtt
     3061 cggtccacgt ggtatttccg tgactgcggt cggtcccggc ccgatggaca cgccgttttt
     3121 ctatggccag gaaacgcctg aacgggtggc ttttcacaag tcgcaaggga tgaacggcca
     3181 gctgaccaaa atcgaagata tcgtgccgat cgtcgggttc cttgctaccg agggttggtg
     3241 ggtcacaggt caaacgatat tcgccaacgg cggatacacc actcggtaac gacgatagag
     3301 catccaacag ccggcgtgca gtcgtagagc agccttcaat ttcgatcgtg agttcggcag
     3361 cagaacggaa atgagatgac ggcatgacca ggagcacatc aggacgacaa tggacaccgc
     3421 cacgagaaaa atacgacggc aaggctcatc ggaattctaa caaggtgctg gttgtcgacg
     3481 tgggcgggac caacgtgaaa atccttgcca ccggcaagcg cgcgccacgc aagatctctt
     3541 ccggtccgaa tatgaccgcg caggacatgg tcgaagcagt gcgaaaactt gccgccgatt
     3601 ggtcctatga cgccgtcgca ctgggctatc caggcgcagt acgggacggc cggccggctg
     3661 cggaacccaa gaacctgggg gatggatgga tgaagttcga tttcgaaaag gcgttcggct
     3721 gtccggtcaa agtgatcaat gatgcggcaa tgcaggctct cggcagctat gacggcggtc
     3781 gcatgctgtt cttggggctc ggcaccggtc tgggctccgc attgatcacc gatggcgtgc
     3841 tggtgccgat ggaacttgcg cacttgcggt atcggaaagg tcgcacatac gaggactatg
     3901 tcggcttgcg cgggctcaaa cggttcggca agaagaaatg gcgtcgatat gtcatggata
     3961 tcgtaacggc gctgaagaat gcgcttcagg ccgaccatgt cgtgctggga ggcggcaatg
     4021 ccaagcggct caagcgtctg ccgaaagatg tgcgcctcgg cgacaacgag catgccttca
     4081 ccggcgggtt tcgcctctgg gaggaggctt ggaaggatac acgcgctgcc tgtgtggtgc
     4141 accagggtcg ttagaaggta ccaaaatatg tatgtggcgg aggagaggag tagaacgtcc
     4201 tgaagggggc gttgcttcgc cccgatttat cggtttatga ggtgagagag ttgcgtttcc
     4261 cctcaaagat ctagatactt cctgctccaa aaagcaaata tcgcggtgtt cctctggcaa
     4321 ggctcttccg gaggccggag tggtggttct atgtatcaat ggagtcgaaa tcttcaacct
     4381 acaatcaagg agggtactgt gagcggtgaa attcaacggt caccggaatg ggtgggatac
     4441 aggagaagga gaacgcacat gcgcaagatg atgattctcg gtttgatggt cctgagcttg
     4501 tcgatgccgg tgtcctatgc cttggccgac caaggaggaa cgcacggaca tggcaagggg
     4561 acccacatga aggtcaccgg cacagtgtcg gccattcaat cggacctcat caccgtgaag
     4621 acgtcgtggg gtcagatcag gatttcctcc gctaccgcgc cgaaaaactt ggaggtcggt
     4681 gaagaagtgg agatgcaggt caacgagaac aatgtcgtgg tcgatgtcca tcgtgcagga
     4741 gatccgtccc attcccatcg cttcgtctcc ggcaagttgg cctatgcgtc aacggacagg
     4801 aaggagatca agctctggac accggagggt aaaaagaagt ttgatgtgca aaccggccgg
     4861 tcccaactct caggcattaa ggaaggcacc ccggtcgccg tcgaactgaa cgaagccggt
     4921 aaagtcatcg atatccatcg catgacggtg gagatagaac tggacgagca tccccacacc
     4981 ctatcgggct accatctgac gttggacgga gtcgtgacga aaattcagtc cggtctggcg
     5041 tttgtgaaga cgccggtggg ccaatacacc attcctatga agcaggctcc acccgatgcg
     5101 gccgtcggcg acaaggtgac gttgtggctc aacgaagaaa atctggtgat cgaccaccat
     5161 gggaaacata agaataaacc ggggacgcac cgtctgatta ccggcaagct gatttatgcc
     5221 ggattgacca agcaggaaat caaactgtgg acaccggagg gcgagaaggt gtttccgctg
     5281 gaccggatgg aagtgaagac caagccgatc gaggaaggct cgatgatcac cattgaattg
     5341 gacgagcagg ggactgtcat cgatctccga aaggcggagt gacaggcgac acggcg
//
LOCUS       DATMTI010000280         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_562314, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000280 DATMTI010000000
VERSION     DATMTI010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562314"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /gene="serA"
                     /locus_tag="VN039_04720"
     CDS             complement(<1..1347)
                     /gene="serA"
                     /locus_tag="VN039_04720"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWV45323.1"
                     /translation="MAAAGMKILISDSLSKQGVEALEKAGFTVVVKSKMPKDELFKEI
                     KDADGLIVRSGTKVTAELIAAAEKLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNT
                     VTTAEHTMSMICAMSRRIPQATASVKTGKWEKDKFMGVELYNKVLGIIGAGQIGSHLT
                     KMAQGIGMSVVAFDPYLAPERAERMGVTMLDLEELFRQADVISVHTPLTPETRGIINA
                     QAIAKMKPGVLIVNCARGGIINERDLCEALKTKRVAAAAFDVFEEEPVKPDNPLLALD
                     NFICTPHIGAQTTEAQENVAIGIAEQIVDYFTKGVAKGAVNIPSVAPELLPRLQPFLT
                     LAEKLGTLQTQLVQGGIERVTVEYSGEVATLSIAPLTIAVLKGLLTPIMEHPVNYVNA
                     PIVAKERGIEVKEVKSTDAGDFTSLIRVRVETGKVSHQVAGTLYHKKEARVTEIEQF"
     gene            complement(1365..>1892)
                     /locus_tag="VN039_04725"
     CDS             complement(1365..>1892)
                     /locus_tag="VN039_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWV45324.1"
                     /translation="DKAWALADKAKNAAFYFNFKKERENQIKNQTAFTPAVSLVIGLQ
                     EAFRMMRSEGLEKMFARQGRLAHAMREGVKAAGLALFPKESPSDALTAVCAPDGIDGQ
                     AIYKNLRVQYGMTAAGGQDHLKGKIFRLSHMGYADTFDVIAALAATEMVLKGLGHPVK
                     LGSGVGKAQEILLAK"
BASE COUNT          351 a          575 c          502 g          464 t
ORIGIN      
        1 aaattgctcg atctccgtga cacgggcttc tttcttgtga tacagcgtcc cggcgacttg
       61 atgtgacacc ttgccggtct cgacccgaac ccgaatcagg cttgtgaaat ccccggcatc
      121 cgtgctcttt acttctttga cttcgatgcc ccgctctttc gccacgatag gggcgttcac
      181 gtagttcacc gggtgttcca taataggagt gagcaagccc ttgagtacgg cgatggtcaa
      241 gggcgcgatg gagagcgtgg cgacttcccc gctgtactcc accgtgactc gctcgattcc
      301 tccttgaacg agttgagttt gaagcgtacc gagcttttcg gccaacgtca gaaagggttg
      361 caagcgaggc aataattccg gagcgaccga cggaatattg acggcgccct tggccactcc
      421 cttggtgaag tagtcgacga tctgttccgc aattccgatc gcgacgtttt cctgagcctc
      481 cgtcgtctgg gctccgatat gcggggtgca gatgaaatta tccaaggcaa ggagcggatt
      541 gtcgggcttg accggctctt cttcaaacac gtcgaaggcc gcagcggcga cgcgctttgt
      601 tttcaaggct tcgcacaaat cacgttcatt aatgatgccg ccccgggcac aattcacgat
      661 gagcacgccg ggtttcatct tggcaatggc ctgtgcgttg atgatcccgc gcgtctccgg
      721 cgtgaggggc gtatggaccg aaataacgtc cgcctgccgg aagagttctt cgaggtccag
      781 catcgtcacg cccattcgct cagcccgttc cggtgccaag tacgggtcaa acgcgacgac
      841 gctcatgccg atcccttgcg ccatcttggt caggtgactg ccgatctgtc cggcgccgat
      901 gatgccgagc accttgttgt agagctcgac acccatgaat ttgtcctttt cccatttgcc
      961 tgtcttcacc gaggcggtgg cttggggaat gcgccgactc atcgcacaga tcatcgacat
     1021 cgtgtgctcg gcagtggtga cggtgttgcc ccccggcgta ttcatgacga cgatgccgcg
     1081 gcgggtcgcg gcaggggtat cgacattgtc caggcccgat ccggcccttc cgacgacctt
     1141 gagtttctcc gctgcagcga tcagttctgc cgtcaccttg gtgccggatc gcacgataag
     1201 tccgtcggca tctttgatct ctttgaacaa ctcgtccttc ggcattttag atttcaccac
     1261 cacggtgaat cccgccttct cgagcgcctc gacaccttgt ttcgacaagc tatcgctgat
     1321 caaaattttc attcccgctg cggccatgtg cgtcctcgcc gtggttactt tgccagtaag
     1381 atttcttgcg cttttccgac accgcttccc aacttcaccg gatggccaag ccctttcaga
     1441 accatctcgg tcgcggctaa tgcggcgatc acgtcgaacg tatccgcata ccccatatga
     1501 gacagtcgga atatcttccc cttgaggtga tcctgtccac cggcagccgt catgccgtat
     1561 tgcacacgca gattcttata gatcgcctgc ccgtcaattc catcaggggc gcacaccgcc
     1621 gtcaaggcat cgctgggcga ttcctttgga aatagtgcca gcccggcagc cttcacacct
     1681 tcgcgcatgg catgggccaa ccgtccctgc cgtgcaaaca ttttttccag cccttccgac
     1741 ctcatcattc ggaacgcctc ttgaaggccg atcacaagcg agacggccgg ggtgaaggca
     1801 gtctgattct tgatctggtt ttcgcgttcc tttttgaagt tgaaataaaa ggcggcattt
     1861 ttggctttgt cggccaaggc ccacgctttg tc
//
LOCUS       DATMTI010000281         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_434994, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000281 DATMTI010000000
VERSION     DATMTI010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434994"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VN039_04730"
     CDS             complement(<1..1295)
                     /locus_tag="VN039_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV45325.1"
                     /translation="MTKTAHILVVDDEVNIRNALVTMLEKKGHRVSGAGTGEEALRHL
                     DETRLNLVITDLRMPGIGGMEFLRRLKAKWPDTEVVVMTAFGSIDTAVEAMRLGAHDY
                     LTKPIDRERFTVIVEKALERHALAFENKQLRDRLETRTRFDHMVGESAAMQRVYSLVE
                     MVADSDVTVLLTGESGTGKELVARAIHHKSPRADGPFITLNCGALPENLFESELFGYE
                     KGAFTGAMSTKVGRFELADGGTLLLDEVGELSLKSQVDFLRVLETKEFRRLGGTKLIK
                     VDTRIVAATNRNLDEAVKQGDFREDLYYRLNVVPLRLPPLRDRADDVPLLVDRYLTEF
                     SAQHHRGPKEVSREAMRLLRLYGWPGNVRQLRNLMERLIVTVKDTTIQPAHLPEEIQA
                     SKEDARTMLITLGTSIEQIERQVIERTLKEVTNHRENAA"
     gene            complement(1292..>1712)
                     /locus_tag="VN039_04735"
     CDS             complement(1292..>1712)
                     /locus_tag="VN039_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV45326.1"
                     /translation="ERQIRLEQVTDEELPFVLGDETQISQVLVNIVVNAFHAMPNGGL
                     CRISAMLREAEEKRWVEIAVKDSGVGIAKKQLFRLFEPFYTTKPSGTGLGLAIAYRIM
                     QDHGGTIQVSSVPGSGTTVVTQFPAAIDQSQKVGVRS"
BASE COUNT          346 a          528 c          429 g          409 t
ORIGIN      
        1 cttgcggcat tttctcgatg gttggtgacc tccttcaaag ttcgttcaat cacttgtcgt
       61 tcaatctgtt cgatagacgt tcccaaggta atcagcatag tccgtgcatc ctctttgctg
      121 gcctggattt cctcaggcag atgcgcgggt tgaatcgtcg tatcttttac cgtgacgatc
      181 agccgctcca tgagatttcg tagttggcga acgtttccag gccacccata gagccgtaac
      241 aaccgcatgg cctctcgcga gacttctttc ggcccgcggt gatgttgcgc ggagaactcg
      301 gtcaggtatc gatctaccag caaagggaca tcgtccgccc gatcgcgaag cggcggaagg
      361 cggagaggga cgacattgag ccgatagtag agatcttccc gaaaatctcc ctgtttgacc
      421 gcttcatcca aattccgatt ggtagcggct acgatccttg tatcgacttt gatgagtttc
      481 gttccaccca gacgcctgaa ctccttcgtt tcaagaacac gcagaaaatc gacttgcgac
      541 ttgagcgaga gttcaccgac ttcatccagc aacaatgtcc cgccgtccgc cagttcgaac
      601 cgcccgacct tggttgacat ggcaccggta aacgcgcctt tctcataccc gaataactcg
      661 ctctcaaaga gattttcagg caaggcaccg caattcaggg taataaacgg tccgtcggcc
      721 ctcgggcttt tatggtggat cgcgcgcgcg acgagttcct tcccggttcc gctttcccct
      781 gtgagcaaga ccgtcacatc gctgtctgcc accatctcca ccaggctata gactcgctgc
      841 atggccgcgc tctcgccgac catgtgatcg aaacgtgttc tcgtttcaag gcggtcacgg
      901 agttgcttgt tctcaaaggc cagggcatgg cgctcgagcg ccttctccac gatcactgta
      961 aaccgctcac gatcgatcgg ttttgtcaga tagtcgtggg cgcccagacg catcgcttcc
     1021 accgccgtgt cgatcgagcc gaaggcggtc atgaccacga cctccgtgtc cggccatttg
     1081 gctttcagtc gacgcaggaa ctccatcccg ccaatcccag gcattctcag atcggtgatc
     1141 accagattca gtcgggtctc gtccagatga cgcagagcct cttctcctgt gcctgccccg
     1201 ctgacccggt ggcccttttt ctccaacatg gtcaccaggg cgttgcggat attcacctcg
     1261 tcatcgacga cgagaatgtg agcagtcttc gtcatgatcg cacaccgacc ttctgcgatt
     1321 gatcaatcgc cgcgggaaac tgagtcacca ccgtcgttcc gcttccaggc acactcgata
     1381 cctgaatcgt accgccgtga tcctgcataa tccggtacgc gatggcaaga ccaagcccgg
     1441 tgccgctcgg cttggtcgtg tagaagggct caaacaggcg gaagagctgt ttcttcgcga
     1501 tcccgacgcc gctatccttc acggcgatct ctacccagcg tttctcttca gcttcccgta
     1561 gcatcgctga aatccgacag agtccgccgt tcggcatggc atgaaacgcg ttgacgacga
     1621 tgttgaccaa tacctgactg atttgggttt catcgcccag cacaaacggt aattcctcat
     1681 ctgtaacctg ttcgagtcga atctgacgtt cc
//
LOCUS       DATMTI010000282         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_562395, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000282 DATMTI010000000
VERSION     DATMTI010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562395"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /gene="hemA"
                     /locus_tag="VN039_04740"
     CDS             <1..1203
                     /gene="hemA"
                     /locus_tag="VN039_04740"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWV45327.1"
                     /translation="AGYGRIQEFLADTHLSLSSEQLTPHLYWHTGDRAIGHLFRVAAS
                     LDSMIIGESQILGQLKDAFEIALAHKTTGVIMNKVVKKAISVAKRVRTETKISETAVS
                     VSYAAVELAKKIFSNLHEKTVLLVGAGEMAKLAAKHLIAHGVGHVRITTRTPQHAVDL
                     AAKFGGTAVPFDQFKDDMGSADIVLVSTGAAHYLVGAEDVHRAVEERMNRPMFLIDIS
                     VPRNIDPAVRHVDNAFLFDIDDLKHRVEQNRAERVQEAEKAERMVLEEVTTMLDWMKS
                     LEVTPTIVALRNRVDDMKRAEVDKVLARLPHLSPQDRELVESLASSIVNKLIHRTMVT
                     LKAEVHSSSGPAFVEAARRFFSLDRQSPSAQPIETYTESSCCRAESADESGAEDPAPE
                     TSIHKRAR"
     gene            1231..2178
                     /gene="hemC"
                     /locus_tag="VN039_04745"
     CDS             1231..2178
                     /gene="hemC"
                     /locus_tag="VN039_04745"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWV45328.1"
                     /translation="MSIQNGQRSTLVLGTRGSKLALCQSEWFQSKVQEVVPEVRVVLK
                     KIQTSGDKIVDVPLAKIGGKGLFVKEIEDALLVGEIDFAVHSMKDVPAQLPECLGILC
                     VPQREDARDALISREGYSFQDLPFGATIGTASLRRQAQLLQARPDLKIAMLRGNLDTR
                     LRKLKEGQFDAIVLAAAGLHRLGWSQTITEYLSPILSLPAVGQGALGIEGRTNDAFVR
                     SVLSRLDHPATHTTVTAERALLRRLEGGCQVPIAAHATLSGERLVLDGLVASVDGKTV
                     IRDRIEGTHQEAHTLGVQLAERLLIRGGDKILRVIYGSA"
     gene            2181..>3282
                     /gene="cobA"
                     /locus_tag="VN039_04750"
     CDS             2181..>3282
                     /gene="cobA"
                     /locus_tag="VN039_04750"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWV45329.1"
                     /translation="MVRRHKGKVYLVGAGPGDPGLLTLRGKECLEQADVVLYDYLANP
                     TLLAYARDRVERIYVGRRGKGKYPEQEAINRLLIERASAGNVVVRLKGGDPFVFGRGG
                     EEAEVLATAGIEFEVVPGVTAAVAAPAYAGIPVTHRTLASTLTIVTGHEDPEKPSTAL
                     DWSRLATGQGTVVFLMGMKNLSTIAATLMAEGQAGSTPVAIIRWGTRVSQQTVVGTLA
                     DIADKADAAQMEPPTVIVVGEVVRLRPKLNWFERRPLFGKRVLMTRAKEQAGELAVRL
                     AAYGAEPVEAPTIKIVPPLDWEPVDHAISEIGTYDWIIFTSVNGVNRFMTRLLACGRD
                     SRCLAGRQLCCIGPRTAQELEKFGVRADLVPTE"
BASE COUNT          720 a          828 c          999 g          735 t
ORIGIN      
        1 gccggatatg gccgtattca agagtttctt gccgataccc atctgtcgtt gtcttccgaa
       61 caattgaccc cgcacctcta ttggcatacc ggggatcgag cgatcggtca cttgttcagg
      121 gtcgcagcca gtctcgattc aatgattatc ggggaatctc aaatcctggg acagctgaag
      181 gatgcgtttg aaatcgcgct ggcccataag acgaccggcg tcatcatgaa caaggtcgtc
      241 aagaaggcta tttcggtagc caagcgcgtg cggaccgaaa caaaaatttc tgaaacggcg
      301 gtgtcggtca gctatgccgc cgtcgaactg gccaagaaga tcttttcgaa tctgcacgag
      361 aagacggtgt tgttggtcgg tgccggtgag atggcgaagc tggcggccaa acatttaatc
      421 gctcatggtg tagggcatgt gcgtatcacg acaagaactc cacagcatgc ggttgatctc
      481 gccgccaagt tcggcggcac ggcagtcccc ttcgatcagt tcaaggatga tatgggctca
      541 gcggatattg ttctcgtctc tacgggggcg gcacattatt tggtcggcgc ggaggatgtg
      601 catcgtgcgg tcgaagagcg catgaaccgt ccgatgttct tgatcgatat ttcggtcccg
      661 cgaaacatcg atcccgccgt tcgtcatgtc gacaatgcgt ttctgttcga tattgatgat
      721 ctgaaacatc gagttgaaca gaaccgagcc gagcgagtgc aagaggcgga gaaggccgaa
      781 aggatggttc tggaagaagt gaccaccatg ctggactgga tgaaatcctt ggaagtcacg
      841 ccgacgatcg ttgcgctcag gaaccgcgtc gatgacatga agcgggcgga ggtcgacaaa
      901 gtgctcgcgc gattgccgca tctgtctcct caggaccgcg aattggtcga aagcctcgcc
      961 tcatcgatcg tgaacaaatt gattcatcgc acgatggtca ccctcaaggc cgaagtccac
     1021 tcttcgagcg gacctgcgtt cgtcgaagcg gcacgtcgtt ttttttcgct cgacaggcaa
     1081 tctccgtctg ctcaaccgat cgagacctat acggagtcgt cctgttgccg tgccgagagc
     1141 gctgacgagt ctggtgccga ggatccggct ccagaaacgt caatccataa gcgagcccgc
     1201 tgatcagtgg gtaacgaaaa gagtctcaac gtgtcgatac agaacggcca acgctctacc
     1261 ttggttcttg ggacgagagg gagcaaattg gcgctctgtc aaagtgagtg gtttcaatcc
     1321 aaggttcagg aggtcgtgcc ggaggtccgg gtcgtgctca aaaagattca gacatccggt
     1381 gacaagatcg tcgacgtgcc attggcaaaa atcgggggga aaggcttgtt tgtcaaggaa
     1441 attgaggatg ccttgctagt cggtgagatc gatttcgctg ttcacagtat gaaggatgtt
     1501 ccagcgcaat tgcctgaatg cctcggaatt ctctgtgttc ctcaacgcga ggatgcccgt
     1561 gatgccttaa tcagtcgtga aggatactcg tttcaggatc tcccgttcgg agccaccatc
     1621 gggactgcaa gtcttcggcg ccaagcgcaa ctgttacaag ccaggccgga cctgaagatc
     1681 gcgatgctgc gcggcaacct ggacacacga ctgagaaaac tcaaagaagg acagttcgat
     1741 gccatcgtct tggcagctgc gggtctgcac cggttgggtt ggtctcagac catcacggaa
     1801 tatctttccc ctatcctcag tctgcctgcg gtcggacagg gagccctcgg tatcgaaggt
     1861 cggaccaacg atgcattcgt gcgttccgtc ttatcccgac ttgatcatcc ggcgacccat
     1921 acgacagtga cggcagaacg ggcgctcttg cgtcgcctgg agggtgggtg tcaggtgccc
     1981 atcgcagccc acgcaaccct gtccggtgag cggctggtct tggatggtct tgtcgccagt
     2041 gtcgatggga agaccgtcat tcgcgaccgg attgaaggga cacatcagga agctcacacc
     2101 ctgggtgttc agttggctga acggctactg attcggggag gggacaaaat tcttcgcgtg
     2161 atttatggat cagcatgaac atggtacgac gacataaagg gaaggtctat ttggtcggag
     2221 ctggtcctgg agatcctgga ctcttgaccc ttcgaggaaa ggagtgtctt gagcaggccg
     2281 acgtggtgct ctacgattat ctggcgaatc ctaccctcct tgcctatgct cgggatcgag
     2341 tcgagcgaat ctatgtcgga cggcgaggta aaggaaaata ccctgagcag gaggccatca
     2401 accgtctgct gattgaacgg gcaagcgcag gcaatgtagt cgtacggttg aagggtggcg
     2461 atccgtttgt ctttgggcga ggaggggaag aagcggaagt gctcgccacg gccgggattg
     2521 aattcgaagt tgttccaggg gtgacggctg cggttgccgc ccctgcatat gccggtattc
     2581 cggtgacgca tcgaacgttg gcgtcgacgc tgacgattgt caccggacat gaagatccag
     2641 agaagccttc cacggctttg gactggtcaa ggctggcgac gggccaaggt accgtggttt
     2701 ttctcatggg catgaaaaat ctttcgacga tcgctgcgac cttaatggcc gaagggcaag
     2761 cggggtctac tcccgttgcg atcattcgat ggggaacgag ggtttcacag cagaccgtcg
     2821 tcggcacctt ggctgatatt gcggataaag ccgatgccgc acagatggag ccgccgaccg
     2881 tcattgtcgt gggagaggtc gttcgcttga gaccgaaact caactggttc gagcggcgtc
     2941 cgctctttgg aaaacgggtg ctcatgacgc gagcgaaaga acaagctggt gagttagctg
     3001 tccgattggc cgcctatgga gctgaaccgg tcgaagctcc gacgatcaag attgttcctc
     3061 cgctcgactg ggagcctgtc gatcacgcga tctccgagat cgggacgtac gactggatta
     3121 tttttaccag tgtcaatggg gtgaaccgtt tcatgaccag gctattggct tgtggacgtg
     3181 actcacgctg tttggctgga cgacagcttt gttgtatcgg gccgcgcacg gctcaagagt
     3241 tggaaaagtt cggtgtcaga gcggatctgg ttccgacaga at
//
LOCUS       DATMTI010000283         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_350408, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000283 DATMTI010000000
VERSION     DATMTI010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350408"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VN039_04755"
     CDS             <1..199
                     /locus_tag="VN039_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HWV45330.1"
                     /translation="HDGRFKTLEEVVNFYNKGGVKNPHQDNLILPLELTDQEKHDLVE
                     FLHTLNGEGWQHVSAPKSFPK"
     gene            complement(257..1111)
                     /locus_tag="VN039_04760"
     CDS             complement(257..1111)
                     /locus_tag="VN039_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45331.1"
                     /translation="MILPPNEWFAIDSSVALDGLKSLILLLTLMIVRSLIVRWIAHNP
                     TLSMESKRRWVVTTRNSVVFAFLIGLVIIWAHELQAFAVSLVALAAAFVLATKELLLC
                     WSGAALRVGGKVYAVGDRIQIAGHRGVVLDHDVFATKLLEIGPGQSAHLYTGRVTIFP
                     NSLLFTNALIKENPEQEYGLYTVVVPIKNDADWQREERILLEAAKIECAPFMEEAVRQ
                     MKLLEKTNLLEAPSPEPRITIQLPESGKIHLVLRFPAPDRGRSRIEQAILRRHLLQMN
                     DQDSLHRT"
     gene            complement(1115..1561)
                     /locus_tag="VN039_04765"
     CDS             complement(1115..1561)
                     /locus_tag="VN039_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45332.1"
                     /translation="MSVSISSAVQKYLPLVTGALLAALAIGFSGCGGPWRDSYFKKGV
                     GRLTQEEIRERLGPPHTAKTPALGGDSLWTYRFALSEQDLTSWNSSFVADASHSVTSM
                     MGKGTELAKPTLYCYRYILTFSEDKTLKNWKREECVPGTRETLTAK"
     gene            1712..>2455
                     /locus_tag="VN039_04770"
     CDS             1712..>2455
                     /locus_tag="VN039_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWV45333.1"
                     /translation="MDVRLIVTACITGVATLSLQTGFAATESRTVKPDSTSPTSLKSD
                     LPHISAKPPGSTVDSKAGERSKSPTNGGAAYQLTVLIAASSEIRGKDGAPMVLVPAGE
                     FVMGSDKGDEDEAPVHRVYLNAFYIDKFEVTNGRFAKYVEAIQSEPPWGFTDKEMPVI
                     HAERPVRWVNWMDAMGYCLWIGKRLPTEAEWEKAARGTDERVYPWGNDPPTPVHAVYG
                     LKEGGAETVSVIGNHHMGQSPYGVQDLAGN"
BASE COUNT          552 a          665 c          724 g          514 t
ORIGIN      
        1 gcacgacggg cgcttcaaga ctcttgaaga agtcgtcaat ttctacaaca agggtggtgt
       61 caagaatccc caccaggaca atctcattct tccgctagaa ctgacggatc aggaaaagca
      121 tgacctggtg gagtttctcc atacgctcaa cggcgagggg tggcagcacg tctccgcgcc
      181 gaaatcgttt ccgaagtaat tcgaaccgtt caggatgagg ggaaccggag ggccatcgcg
      241 agatggccct cttttgttag gtgcggtgta aggagtcttg atcgttcatc tggaggagat
      301 gccggcgtag aatggcctgc tcgatacgcg agcgtccccg atctggtgct gggaatcgga
      361 gtacgagatg tatcttgcct gattccggta gttgaatggt gatgcgcggt tccggggaag
      421 gcgcttccag caaattggtc ttctccaaca gcttcatctg ccgcactgct tcttccatga
      481 acggcgcaca ttcgattttc gccgcctcca agagaatccg ctcctctcgt tgccaatccg
      541 cgtcgttttt gatgggtacg acgactgtgt agaggccgta ttcttgctca ggattttcct
      601 tgatcaacgc gttcgtgaac aacaggctgt tggggaaaat cgtcacacgg ccggtgtaaa
      661 gatgagcgga ttggccggga ccgatctcca ataacttcgt cgcaaagacg tcgtgatcca
      721 gcacgactcc gcggtggccc gcgatttgaa ttcgatcacc gacggcatag actttgccgc
      781 ccactcgaag tgccgcgccg ctccagcaga gaagcagctc tttcgtcgcc aagacgaacg
      841 ccgccgccaa ggcgacgagc gaaacggcaa aggcttgcag ttcgtgggcc cagatgatta
      901 cgagaccgat gagaaacgcg aagacgaccg agttcctggt cgtgaccacc cagcggcgtt
      961 tcgattccat cgagagcgtc ggattgtggg cgatccaacg cacgatcagc gacctgacaa
     1021 tcatcaaggt cagcaacagg atcagcgact tcaatccgtc caacgcgacc gagctgtcga
     1081 ttgcaaacca ctcatttggg ggcaatatca tcctctactt ggcggtcaat gtttcgcggg
     1141 tacccggcac gcattcctca cgtttccagt tcttcagggt cttgtcttcg gagaacgtca
     1201 agatgtaccg atagcaatac aacgtgggtt tggcgagttc ggttcccttg cccatcatgc
     1261 tggtgaccga gtgagaagcg tcggcgacga acgaggaatt ccacgaggtg aggtcctgtt
     1321 cgctgagtgc gaaccgatag gtccagagac tgtccccgcc gagagccggg gtcttggccg
     1381 tgtgcggggg acccagtcgc tctcgaatct cctcctgtgt cagccgacca actccttttt
     1441 tgaaataact atctctccag ggaccgccac agccggaaaa gccgatggcc agagcggcaa
     1501 gcagggcacc ggtaacaagg gggaggtact tttggacggc ggaagaaatc gagaccgaca
     1561 tgctgacgct tacgctacac cggacgaccg gtaagtgcaa ctccatagac catcaccgct
     1621 atcatgttcg gccggcggta aagcagcaag ccgaggcggg ctggatttgg ccatacgttt
     1681 tcggtacctt agtattggcc gaggccaacg tatggatgtc cgactgattg ttaccgcgtg
     1741 cattaccggt gtcgcgaccc tctctctaca gacggggttt gcagcgaccg aaagccggac
     1801 cgtcaagccc gactccactt caccaacttc cctgaagtct gatcttcctc acatatcggc
     1861 gaaaccacca ggctccactg tcgattccaa ggctggagag aggagcaagt cgccgaccaa
     1921 tggtggcgct gcatatcagc tgactgtgct gatcgcggcc tcttccgaaa tcagggggaa
     1981 agatggtgcg ccaatggtgc tggtgccggc cggtgagttt gtgatgggaa gcgataaggg
     2041 tgatgaagac gaagcccctg tgcaccgtgt gtatctcaat gccttctaca tcgacaaatt
     2101 cgaagtgacg aacggacgat tcgccaagta tgtcgaggcc atccagagtg aacctccctg
     2161 gggatttacg gacaaggaga tgcccgtgat ccatgccgag cgtccggtcc gctgggtgaa
     2221 ctggatggat gcgatggggt actgcctctg gattggcaag cgacttccca cagaagccga
     2281 atgggagaag gctgcgcgtg gaactgacga aagggtctac ccatggggca atgatccacc
     2341 gactccggtg catgcggtct atgggttaaa ggaaggcggc gcagagacag tgtcggtcat
     2401 cggcaatcac cacatggggc aaagtccata cggtgtacag gatctggcgg gtaat
//
LOCUS       DATMTI010000284         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_11024, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000284 DATMTI010000000
VERSION     DATMTI010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11024"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VN039_04775"
     CDS             <1..1039
                     /locus_tag="VN039_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV45334.1"
                     /translation="WSIILMALPTCLVGLLPTYAQIGLVAPLALTALRFLQGLSVGGE
                     FTGSVTFLVEHASRTERGYIGSWAGFSAQIGALLGSGIGTLTTVSLSSEALQEWGWRM
                     PFVAGSVIALVGWYLRRRIPESPDFERLQQAGVVSSSPVRELFVSHRAPLFQVIGLVL
                     LHGVGFYIFYVFLPTYLTKVTDLPMGTALLINTMCMALLAMLIPFMGKLSDRVGHRWV
                     LAAGAAGLALGTVPFFSWMSSGHLVLIVAASVLITVFVSAYMGPFFAITATLFPVAQR
                     YTGLSASYNIASALFGGTAPLMATVMMERSGSVLAPGWYVSLCAVLSLIALSTIREEL
                     KGTVEVPAGTH"
     gene            1089..2504
                     /locus_tag="VN039_04780"
     CDS             1089..2504
                     /locus_tag="VN039_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWV45335.1"
                     /translation="MIDQTTPPPALSGPSVSRWRGCFHEFRRDTLGFLLRCHAYGDVV
                     KLPMGLVVDLLLGQRDAAMYVLNHPADIKHVLVTNQDNYRKAPVPPVESRVFGQGVLH
                     TEGETHHRQRRLFLPFFHGNHVTAYTDLITGKAHDLVGHWQNGTTVDIEHEMAHLTLS
                     VIWRLLFGRDVGSEADTIREAITVGQSLIKLQYDSLLASCTPLWVPTKLHRRFTRGHR
                     SMEAMIRRMIKERRTASHQNDDVLALLLAARDTDGRLLSDKEIHDELVTFLLAGHETT
                     ANALTWTWFLLAQFRPVQERLMCELNEALGGRLPTAADVPRLRYTKMVWEESLRLYPP
                     AWSLHTRVAHAKDRLPSGAVLPSRAWVFISPWSLHRNERWFPDPNRFDPERFSEEAIR
                     TRPPFSYIPFGAGGRRCLGESFAELEGLLILATIASKVRLRLVSGQTIQPEPVMTLRP
                     DVSVSMIVQRVAIAEPLSAHA"
BASE COUNT          478 a          741 c          753 g          688 t
ORIGIN      
        1 ctggtccatt atcttgatgg cattgccaac ctgcttggtc ggcctactgc cgacctatgc
       61 tcagatcggt cttgttgcac cgctcgcact cacggccctg cgctttcttc aaggtctatc
      121 ggtcggtgga gaattcaccg gctccgtgac cttcctcgtt gaacatgcct cacggaccga
      181 gcgaggctac atcggcagtt gggccgggtt cagcgcacag atcggggcgc tgttgggttc
      241 cggtatcggc accttgacga ctgtgagcct ctcatcggag gcactccagg aatggggatg
      301 gcgcatgccg ttcgtggcgg gaagtgtcat cgcgttggtc gggtggtatc ttcggcggcg
      361 cattcccgag tcgccggatt tcgaacggct gcaacaagcc ggtgtggtgt cttcctcgcc
      421 ggtccgtgag ttgttcgtat cgcaccgagc gccgctattt caggttattg gtctcgtctt
      481 gttacatggc gtcgggttct acatcttcta tgtgttcctc cctacttatc tgacaaaagt
      541 gaccgacctc ccgatgggaa cggcactgct gatcaacacg atgtgtatgg ctctgttggc
      601 gatgctgatt ccgttcatgg gcaagctgtc cgatcgagtc gggcatcgat gggtattggc
      661 cgccggtgcc gcaggtcttg ccctcggcac ggtcccgttc ttttcttgga tgagcagcgg
      721 ccatcttgtg ctgatcgtcg cggcttcagt cctgattacg gtgttcgtgt cggcttatat
      781 gggtccgttc tttgccatca cggcgactct ctttccagtc gcgcaacgct acacgggtct
      841 ttcagcctcc tacaatattg cctcggccct gttcggaggg acggcaccgt tgatggccac
      901 agtgatgatg gaacggagcg gaagtgttct tgctcctgga tggtatgtca gcttgtgcgc
      961 cgtcctatcg ttgatcgcat tgtccacgat tcgtgaggaa ctgaaaggga ccgtcgaggt
     1021 tccggctgga acccattgac ggtggcgacg acacatacgg tagtttttat gagcgacacg
     1081 aaggcaacat gatcgatcag acgactccac cacctgccct ttccggtcca tcggtctccc
     1141 gctggcgagg atgttttcac gaatttcgcc gtgacactct cgggtttctc ttgcgctgtc
     1201 atgcctatgg ggacgttgtc aaacttccga tgggtcttgt cgtcgacctt ctcttagggc
     1261 agcgcgacgc tgccatgtat gtgctgaacc atccggccga catcaagcat gtgctggtga
     1321 ccaatcaaga caattatcgg aaggcgccgg tccctcctgt cgaatctcgg gtatttggtc
     1381 aaggtgtgct ccacacggaa ggcgagaccc atcaccgcca gcgacggttg tttctcccct
     1441 tctttcacgg aaatcatgtt accgcgtaca ccgatttgat tacgggcaag gctcatgacc
     1501 tcgtgggaca ttggcaaaac ggaacgacgg ttgatattga acatgagatg gcgcacctga
     1561 ccctgtctgt gatctggcgc ctactctttg gacgggacgt cgggtccgaa gccgacacga
     1621 ttcgggaagc cattacggtc gggcaaagtt taatcaaatt acagtatgac tccctgttgg
     1681 cgagctgcac tcccctgtgg gttccgacga aactccatcg ccggttcacc cgaggtcacc
     1741 gatcgatgga agcaatgatt cggcggatga tcaaggagag gcgtacggcg tctcatcaga
     1801 atgatgacgt gctggcgttg ttgctcgccg cgagagatac ggatggacgt ctgctgagtg
     1861 ataaggagat ccatgacgaa cttgtgacct tcttgctcgc cggtcatgaa accaccgcta
     1921 atgcgctgac ctggacctgg tttttgctgg cgcaatttcg accggtacag gaacggctca
     1981 tgtgcgaatt gaatgaggcg ttaggtggcc gtcttccaac tgctgctgat gtgcctcgct
     2041 tgcggtacac gaagatggtc tgggaagagt cgcttcggct ctatcctccc gcgtggagcc
     2101 ttcatacccg tgtcgcccat gcgaaggacc ggcttccgtc aggtgccgtg ctgccatccc
     2161 gtgcctgggt ctttatcagt ccttggagtt tgcaccgtaa tgagcgatgg ttcccggatc
     2221 ccaaccgatt tgatcccgag cgattttcag aagaagcgat ccggacccgt ccaccgttca
     2281 gctacatccc tttcggcgct ggtggacgtc ggtgccttgg agagtccttt gcggagttgg
     2341 aaggcctgtt gatcttggcg acgattgcgt ccaaagttcg gttgcgcctg gttagtggtc
     2401 agacgatcca accggaacca gtcatgacac tacgccccga tgtttcggtc tcgatgatcg
     2461 ttcaacgggt cgctattgcg gagccgcttt ccgctcatgc ctaaatccct ccgctcgctc
     2521 catttcattt cttctaggca tctctttttt acaagtcgcg tccgattgtt gctgaactag
     2581 ctataacgca cttgatgcag acattgacat cacaactttc actttggttt ttccttgctg
     2641 ctcggtcggg aacaattccc
//
LOCUS       DATMTI010000285         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_393023, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000285 DATMTI010000000
VERSION     DATMTI010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393023"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VN039_04785"
     CDS             complement(<1..769)
                     /locus_tag="VN039_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWV45336.1"
                     /translation="MTVGQLVTGLLVGVALCVSHAGTFAQTAGNLIIVGNGPEQTTIE
                     ALARAFEKANPRAYVDIQWDEDSKPLQSVKTGQAHIAVTGVEDPALAATQIGWDGIGI
                     LVHLSNFTKEVTKQQVADIFSGKIKEWSDVGGPETKILLINRPRNQNVREAFETQLGI
                     ADKIPESTKMIGVDEAVVKTVVGTLPPLSAVAYISLSTGLSVVSGGVAVRLLPVDKVE
                     PEVPTVKDGRYSLRRPLLLLSKKEPNTLVEAFAQFALS"
     gene            complement(819..1133)
                     /locus_tag="VN039_04790"
     CDS             complement(819..1133)
                     /locus_tag="VN039_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWV45337.1"
                     /translation="MPLYEYHCGQCEKQFEANQSVYARVEDTECPFCRARQATRLMSS
                     FSSNIVGDRKPGFSELKAKAMNEERLQRFAKLPPLNIRPNMLSPHVTSESEPPSTEGS
                     TS"
     gene            1285..1461
                     /locus_tag="VN039_04795"
     CDS             1285..1461
                     /locus_tag="VN039_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45338.1"
                     /translation="MPVLIRRYKGNNGMMQEERIDDDDRIERYMRLFEKDDVKKLETG
                     VKVFIEKDEWQLLP"
     gene            1535..1738
                     /locus_tag="VN039_04800"
     CDS             1535..1738
                     /locus_tag="VN039_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45339.1"
                     /translation="MIYWVHIARAIDRVTLHRDRCSEVPSTATSSDFWEGGWFDYPDK
                     ERALRAMEQAGTNDQRRCPLCKP"
     gene            1838..2662
                     /locus_tag="VN039_04805"
     CDS             1838..2662
                     /locus_tag="VN039_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWV45340.1"
                     /translation="MPRLALLLTTVIWGATFPATKAALEQIPPLSFLLLRFLLGTLLV
                     LLWFVVCRRRLHRDRVVLGASALTTVFLFLGYLLQTVGLRHTSASNSAFLTALYVIFV
                     PMILMRIDSRVMLATAIAVVGLWLLVKPDTSMNLGDLMTLGCAVAFAGHIICLERFTR
                     QVDAPSLFVWQMMAMTLLFLPAPWWEETTQSDFSPTAVLLIGLGVTGILATLAFAVQI
                     WAQRLVPAQQVALLFASEPAYAAWLSWYFLGETLDVQGWIGSALILLAVAIGAFGA"
BASE COUNT          560 a          712 c          805 g          670 t
ORIGIN      
        1 gagagagcgc aaactgtgca aaggcctcaa ccagcgtgtt cggttctttt ttcgaaagga
       61 gcaacagagg gcgacggagg ctgtagcggc cgtccttcac ggtcggcact tccggctcga
      121 ccttatccac cggcaacaaa cggacggcga cgccgcctga gacgacggag aggccggtgc
      181 tcaatgaaat ataggcgact gcggagagag gcggcagggt gccgacgacg gtcttcacga
      241 cggcctcatc aacgccgatc attttggtgg actctggaat tttgtccgct atcccgagtt
      301 gagtttcgaa ggcttcccga acgttctggt tacgcggtct gttgatcagg agaatcttag
      361 tctccggtcc gcccacatcc gaccactcct tgatttttcc tgaaaaaatg tcggcgactt
      421 gctgcttcgt cacctctttg gtgaaattcg ataaatggac caaaatgccg attccatccc
      481 aacctatctg agtcgccgct aaagccggat cttcgacccc ggtgacggca atatgggcct
      541 gacctgtctt gaccgattgc aatggtttgg aatcctcgtc ccactggatg tcgacatagg
      601 cacggggatt ggctttctca aaggctcggg ccaaggcctc gatggtcgtt tgttccggcc
      661 cgttgccgac gatgatcaga tttccggctg tttgagcgaa ggtgccggca tgtgacacac
      721 agagagccac tccgacgagc agcccggtta cgagctgtcc cacggtcatc gagcggcctg
      781 tccccactgg aggtcacgac cgggttggtg ttgggtgctc aagaggtcga tccttccgtt
      841 gacggaggct ctgattctga ggtgacgtgc ggcgacagca tgttaggtct gatattcagt
      901 ggcggcaact tggcaaagcg ctgcagccgt tcctcgttca tggccttggc tttcagctca
      961 gaaaagccgg gcttacgatc accgacgata ttggacgaaa acgaagacat gagtcgagtt
     1021 gcctggcggg cccggcaaaa tggacattcc gtgtcttcga ctctggcata cacggactga
     1081 ttggcctcga attgtttctc gcactgtcca caatggtatt cgtagagcgg cacggaattg
     1141 gatcctttct gtgtctctat tgcagagata tcggttcagg acttgacaag gcgacctctc
     1201 ccaagcgatc ttgaccggct cgtaacgata cgtttattct agcaacagac gcaactaaac
     1261 tgccagcttt ataggagggt ttcgatgcca gtgctgattc gacggtacaa aggtaataac
     1321 ggcatgatgc aggaagaacg aatcgacgac gacgatcgaa tcgagcgcta catgaggctc
     1381 ttcgaaaagg acgacgtcaa gaagttggaa accggcgtga aggtctttat tgagaaggat
     1441 gagtggcaac tcctccccta gccggtagga ggtgagggga tgggagttag acggatcgtc
     1501 tgcaccctca ccacaagcac ctcactggcc ccgcatgatt tattgggttc acattgcacg
     1561 cgcgattgac cgtgtgactc tccaccgaga ccggtgttcg gaggttccat ccaccgcgac
     1621 ttccagtgat ttctgggagg gaggatggtt tgactatccg gacaaggaac gggcgctccg
     1681 tgccatggaa caggccggaa ccaatgatca gcggcggtgc ccgctctgta agccgtagaa
     1741 atataaaagt gctgagttct gggtgctgag tttaaagtca aagaaatggg cgactatgtt
     1801 cagcactgag cattcaaaac tcagcactct ttaattcatg ccgcgacttg cccttttact
     1861 gactacggtc atctggggtg ccacatttcc cgcgaccaaa gcagcgcttg agcagatccc
     1921 tccgctttct ttcttattgc tccggttcct cctaggcacg ctcctggtgc ttctgtggtt
     1981 tgtggtctgc cgtcggcgac tgcaccgtga tcgtgtcgta ttaggagcga gcgcgctcac
     2041 gaccgtcttt ctctttctcg gatacctgtt gcaaacggtc gggctccgcc atacgagcgc
     2101 ctccaattca gcctttctga cggcgttgta cgtgattttt gtgccgatga ttctcatgcg
     2161 gatcgacagt cgggtaatgc tggcgacggc gattgcggtg gtcgggctct ggctgttggt
     2221 taagcccgat acctctatga atctgggaga tctcatgacg ctagggtgtg ccgtcgcgtt
     2281 tgccgggcat atcatttgtc ttgagcggtt caccagacag gttgatgctc cgtcactatt
     2341 tgtatggcag atgatggcga tgacgctgtt gttccttccg gctccctggt gggaagagac
     2401 gacgcagagc gacttttcgc ccacggccgt cttactgata ggccttggtg tcacgggtat
     2461 tctggccacg ctggcatttg ccgttcagat atgggcgcag cgactggtgc ctgcccaaca
     2521 ggtggcgtta ttgtttgcgt ccgaacctgc ctacgcagcc tggttgtcct ggtactttct
     2581 tggtgagacg ttggatgtgc aaggatggat cgggagcgcg ctgatcttat tggcggtggc
     2641 gatcggggcg ttcggcgcct aatcgtttca ttaaggagga atcgtatggc gcagaagttc
     2701 ggtaacggac gatgggttcg cgaaggattt ttggataacc gcgtcga
//
LOCUS       DATMTI010000286         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_52938, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000286 DATMTI010000000
VERSION     DATMTI010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52938"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..451)
                     /gene="rplM"
                     /locus_tag="VN039_04810"
     CDS             complement(11..451)
                     /gene="rplM"
                     /locus_tag="VN039_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWV45341.1"
                     /translation="MVNAKTYSQKPVEVTRRWILVDASEAPVGRVATQIATYLTGKYK
                     PTYTPHIDGGDYVVVINAKDAVFTGKKETDKKYYRHSGFPGGIKDATVQEVREKAPER
                     LLEAAVRGMLPKNKLAAERMKRLRVFAGSDHAHTAQSPEKVEVK"
     gene            complement(451..843)
                     /gene="rplQ"
                     /locus_tag="VN039_04815"
     CDS             complement(451..843)
                     /gene="rplQ"
                     /locus_tag="VN039_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWV45342.1"
                     /translation="MHRHGYKGRKFGRERDQRRALLKGLATSLVEHGKIETTLPKAKE
                     TARYIEKLITKAKKGDLANRRQVIAKLSTQAAAFKLVDEIAPQLTGRNSGHVRVVRTR
                     LRVGDGAQLATVSFVDELKDAPKKEVKA"
     gene            complement(845..1768)
                     /locus_tag="VN039_04820"
     CDS             complement(845..1768)
                     /locus_tag="VN039_04820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWV45343.1"
                     /translation="MSKVIHNPALANVEDHGATSATFTIEPLQAGYGNTLGNSIRRVL
                     LSSVRGGAIVAFRVEGATHEFTTVKGVKEDVVDIMLNLKNVRLKVHTDEPVELRLEKK
                     GAGAVTAGDIKTTADVEIVNPEQVIATIDDPKASVVMDLVAEAGRGYRTIEDSSTSRV
                     HSDMIALDAVFTPVLRVRYKVAGTRVGRETNLDKLTLTVDTDGSVSPRDAFEEAAAIL
                     VNQYTALAGSTTVEAAPALGQQDEEESNELNTPIEDLNLTARTANALINNDIRTVHDL
                     VTLSEQDLRELKGFGSKALDEVKDKLAELEL"
     gene            complement(1786..2403)
                     /gene="rpsD"
                     /locus_tag="VN039_04825"
     CDS             complement(1786..2403)
                     /gene="rpsD"
                     /locus_tag="VN039_04825"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWV45344.1"
                     /translation="MARDTSPIVKQSRREGYALHPKAHKILAKKSGIPGQHAHGRQGK
                     QSLYATQLREKQKVRRLYGLLERQFAKLMNEAGRKQGLTGENLLQLLELRLDNTVYRA
                     GFATSRRAARQLVGHGHFELNGQRVDIPSIRVKPGDEIVVRAKSAKSGYFAQIDDVVN
                     NSVQGPLSWLKSDHKKLKISVTGLPKREEAEADINEQLIVEYYSR"
     gene            complement(2407..>2665)
                     /gene="rpsK"
                     /locus_tag="VN039_04830"
     CDS             complement(2407..>2665)
                     /gene="rpsK"
                     /locus_tag="VN039_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWV45345.1"
                     /translation="SAGACGFRGSKKGTAYAAQVAAEKAAENVQALHGTKAVDVFVKG
                     VGLGRDAAIRALGSFDITVESIKDVTGVPHGGVRPKKARRV"
BASE COUNT          550 a          699 c          678 g          738 t
ORIGIN      
        1 tcagccatga ttatttaacc tcaactttct ctggtgattg cgcagtatga gcgtgatcac
       61 tgcctgcaaa aacgcgtagt cgcttcatgc gttcagcagc aagcttgttc tttgggagca
      121 taccacgaac cgccgcttcc aacagacgct ctggcgcctt ctcgcggact tcctgaacgg
      181 ttgcatcttt gatgccgccc ggaaagccac tgtgacggta gtatttctta tcggtttctt
      241 ttttaccggt gaagaccgcg tcttttgcat tgataacgac gacatagtca ccgccatcaa
      301 tgtgtggtgt ataggttggc ttatatttac cagtcaggta ggtcgcgatc tgtgtcgcga
      361 cacgtccgac aggcgcttct gaagcatcaa ccagaatcca acgacgagtc acctcaacgg
      421 gtttttgtga ataggtcttg gcatttacca ttacgctttc acctccttct tgggtgcgtc
      481 cttgagctca tcaacgaatg agacagtcgc aagctgtgcg ccgtcgccga cacgcaggcg
      541 tgtgcgaacg acacggacat ggccgctgtt gcgtccagtc agttgcggtg cgatttcatc
      601 aaccagcttg aatgcggctg cctgagtcga tagcttcgca attacctgtc gtcggttcgc
      661 tagatcaccc tttttagctt tcgtaatcaa cttttcaatg taacgtgctg tttcctttgc
      721 ttttggcagg gtggtttcga tcttgccatg ttcgacaaga ctggtagcca gcccctttag
      781 cagagcacgt cgttggtcgc gttcacgacc gaacttccgt cctttgtaac cgtgtcgatg
      841 catattacag ctccaactcc gcgagcttgt ctttgacttc gtcaagcgcc tttgaaccaa
      901 agcccttgag ttcgcgtaga tcctgctcac ttaatgttac gaggtcgtgt acagtgcgga
      961 tatcattgtt gataagcgcg ttggcagtac gggctgtcaa gttgaggtct tcgataggag
     1021 tgttgagctc gttcgattct tcttcgtctt gttgaccaag tgctggtgct gcttcaacgg
     1081 ttgtgctgcc tgcaagtgcc gtatattgat tgaccaggat tgcggcagct tcttcaaatg
     1141 catcacgtgg tgaaactgaa ccatcagtat caaccgtcaa cgtgagtttg tcgaggtttg
     1201 tctcacgacc gacacgggta cctgccacct tgtaacggac acgtagtacc ggagtaaata
     1261 cggcatcaag cgcgatcata tcgctatgga cgcgtgatgt gctcgagtct tcgatcgtgc
     1321 ggtagccgcg accagcttca gcgacaagat ccatgacaac tgatgccttt ggatcgtcga
     1381 tggtggcgat aacctgttca gggttgacga tctcaacatc ggcagtcgtc ttgatgtcgc
     1441 cggccgtaac ggctcctgcg ccttttttct cgaggcgtag ttcgacaggt tcatcagtat
     1501 gtaccttgag tcggacgttc tttaggttga gcatgatgtc gacgacatct tctttaacac
     1561 cttttacggt tgtgaactca tgagtcgcgc cttcgacacg gaaagcgacg atcgcaccac
     1621 cacgaacact cgaaaggagt acccggcgga tgctgttacc gagcgtgttg ccgtagccag
     1681 cctggagcgg ttcaatcgta aaggttgcac tagttgcgcc gtgatcttcg acgttcgcaa
     1741 gtgcaggatt gtgaataact tttgacatat cttcagctcc ttaccttatc gtgagtagta
     1801 ctcaacgatg agttgttcat taatatcagc ttcggcctct tcacgctttg gaagtccggt
     1861 aacgcttatt ttcaattttt tgtgatcgct cttgagccag ctcaacgggc cttggacgga
     1921 attgttgacg acatcatcga tctgcgcaaa gtaaccagac ttggcacttt tggcgcgcac
     1981 gacgatttca tcacctggtt taacccggat cgatgggata tcgactcgtt ggccattcag
     2041 ttcgaagtgg ccgtgaccga cgagctgacg tgcagcccgg cgactggttg cgaaaccggc
     2101 gcgataaacg gtgttatcaa gacgcagttc aagaagctgc agcaggtttt cacctgtcag
     2161 accttgttta cgtccagctt cgttcatcag cttggcgaat tgtcgttcaa gtagaccgta
     2221 caaacggcgt actttctgtt tttcacgcag ctgggtcgcg tacaagctct gcttgccttg
     2281 acggccatgg gcgtgctgac ctggaatacc agatttcttt gccaaaatct tgtgagcctt
     2341 cgggtgaagc gcgtagcctt cgcgacggct ctgtttgacg ataggagatg tatctcgtgc
     2401 catatcctag accctccgtg ctttcttcgg acgaacgcca ccgtgtggta cgcctgtcac
     2461 atctttaatg ctttccaccg taatgtcgaa cgaaccgagt gcgcgaatcg ctgcgtctcg
     2521 tcccagtccg acacctttta caaacacatc gacggctttt gtgccatgaa gtgcctgaac
     2581 attctcagct gctttttcag cggcgacctg agcggcgtat gcggtacctt ttttactacc
     2641 gcggaaacca caggcaccag cgctt
//
LOCUS       DATMTI010000287         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_689582, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000287 DATMTI010000000
VERSION     DATMTI010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689582"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VN039_04835"
     CDS             complement(<1..707)
                     /locus_tag="VN039_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWV45346.1"
                     /translation="MLVDTHCHIHENGYPLDIGDTITRAHHAGISHLICVGTSEQSSK
                     EAVAFALQHEQVFAAIGVHPHDTKDGYGLVRELASAEHVVAVGEIGLDYFYTHSPREV
                     QIAALEAQLQIARDHDLPVIFHVREAFDDFWPIFRNFSQLRGVLHSFTDSVQNMEKAV
                     SNGLFIGVNGISTFARDKQTMFDAVPLEKMLLETDAPFLTPVPYRGKVNEPAYVRVVA
                     EYHASRRDISLEEIAAAT"
     gene            complement(707..>1534)
                     /locus_tag="VN039_04840"
     CDS             complement(707..>1534)
                     /locus_tag="VN039_04840"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWV45347.1"
                     /translation="QVMYVWLDALSNYITVLGYPDDVTWQQFWPADVQVIGKDILRFH
                     AGIWPPMLLGLGLPLPKKLLVHGFVNVGGAKMSKTVGNVIDPNEIIDGYGIDAFRYYF
                     SRHIPTQDDGDFTWERFESAYNTELGNDLGNLVQRVGSMVTRYQAGVIGDIKQGEHDV
                     LRYHEYMEQLQFNRALDEVWNMVRSLNQYIEEVKPWEIAKTREKDTDAEGHLSDVLAH
                     AVGTLLQIADLLLPFLPTTAKAIHASFETGVVVPQGVLYPKIYNHTPDPRTDAAPKK"
BASE COUNT          356 a          406 c          407 g          365 t
ORIGIN      
        1 gtggccgccg ctatctcttc aagagaaatg tcgcgtcgtg aagcgtgata ctctgctacc
       61 accctcacat acgctggctc gtttacttta ccacgatatg gtacgggagt caagaaggga
      121 gcgtcggttt ccaaaagcat tttttccaga ggtacagcat cgaacattgt ttgtttgtcg
      181 cgggcaaatg tcgagatgcc gtttaccccg atgaacagtc cgttcgaaac ggctttttcc
      241 atgttctgga ccgaatcggt aaaactgtgc aatacgccgc gtaactggga gaaattgcgg
      301 aatatcggcc agaaatcgtc gaaagcctcg cggacgtgaa agataacggg tagatcgtgg
      361 tcccgggcaa tctggagttg cgcttccaga gctgcaatct gcacctcacg tggactatgc
      421 gtgtaaaagt aatcaaggcc gatctcgccc accgcgacga cgtgctcagc agacgcaagc
      481 tcgcgaacga ggccgtagcc gtctttggta tcatgcggat gtacgccgat ggccgcaaac
      541 acctgttcat gctgcaacgc gaacgcaacc gcctctttag agctctgctc actggttccg
      601 acacagatga gatgcgagat acccgcgtga tgcgcccgcg tgatcgtatc accgatatcg
      661 agcggataac cgttttcgtg tatatgacag tgggtatcaa caagcattac tttttcggtg
      721 ccgcgtcagt tcgtggatcc ggcgtatgat tgtatatctt cggatacagt acgccctgcg
      781 gaacgacgac accggtctca aacgaagcat ggatcgcttt ggcagtggtt ggcaggaatg
      841 ggagcaacag atcggcaatc tgcaagaggg taccgaccgc atgtgccagt acgtcgctga
      901 ggtgaccttc ggcatcagtg tctttctcgc gtgttttggc gatctcccat ggtttgacct
      961 cttcgatgta ttggttcagg ctgcggacca tattccagac ctcatcgagt gcacggttga
     1021 actgcagctg ctccatgtat tcgtggtaac gcagcacgtc gtgctcaccc tgtttgatat
     1081 cgccgatgac tccggcttga taacgcgtca ccatgctacc gacgcgctgg acaagatttc
     1141 caagatcgtt cccaagctcg gtattataag cgctttcgaa gcgctcccat gtaaaatcac
     1201 cgtcgtcttg agtaggaata tgacgcgaga agtaatatcg gaaagcatcg atcccatatc
     1261 catcgatgat ctcattcgga tcgatgacgt tgccgaccgt cttactcatt ttggctccgc
     1321 cgacgttcac gaagccgtgg accaacaatt tctttggcaa cggcaatcca aggcccagca
     1381 acatcggcgg ccaaataccg gcatggaagc gtaagatgtc tttaccgatg acttgtacgt
     1441 cagccggcca gaactgctgc caggtgacgt cgtccggata gcctagtacg gttatgtagt
     1501 tggaaagtgc gtcgagccag acatacatca cctg
//
LOCUS       DATMTI010000288         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_308331, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000288 DATMTI010000000
VERSION     DATMTI010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308331"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VN039_04845"
     CDS             complement(<1..266)
                     /locus_tag="VN039_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45348.1"
                     /translation="MILSRREFSRGATGLIFCATSASALLPKASMAGSKRRYSFIQPG
                     AEWLDTNGKPIQAHGGSLIAVGDSYYWYGENKEFTIGKDSPQSWG"
     gene            complement(383..1615)
                     /locus_tag="VN039_04850"
     CDS             complement(383..1615)
                     /locus_tag="VN039_04850"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV45349.1"
                     /translation="MQNNFLGSLARIRLIHALPAAAAMLMLLSSGSATAGQSSTEVTE
                     ATPTYSANSPLGELVDNPSTLAILQKFVPEVVNNPLYLASRTISLQVVQRLSKGMISA
                     DEVKLADVKLRTVPEIKIERPLNGAADDFLEIRTFRLWSGPAPLSSSLDDQPTLTVMP
                     AEGAPTGTAVIIAPGGAYLGVAAGLEGREVADWFAARGVTAFVLKYRVGRNNPMPVPL
                     LDAQRAVRLVRSRAREYGIAPDRIGIMGFSAGGHLAALEATLGEAGNPNSTDVVDRAD
                     SRPNFVVLGYPALNMFSPAEKTIPYCRLMAIESECDSAWYARYRPEDHVDAKTPPMFI
                     YHTTTDELVPVGGSIGFYRLLTQSHVPAELHVFGTGRHGSGLGKGDPELDQWPALLEA
                     WLRDNGWLSPYTFPSSKK"
     gene            complement(1605..>2928)
                     /locus_tag="VN039_04855"
     CDS             complement(1605..>2928)
                     /locus_tag="VN039_04855"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HWV45350.1"
                     /translation="RLDNLTLRRDAYPLKAKGYFRSSDPELDNIWDIGWRTLQIDAHE
                     TFMDSAYWEQLQYVGDSRLESLIALSVSGDPRLPAEAIDAFGESVRQDGMIQSAYPSN
                     SNNIIPTFGLLWIGMMHDYWMQRPDRAVIIRNLPGARKILTWYAPYVSNNGLLRRNPE
                     WNFVDWVGDPPLARDKFPSFDPTSGTSCLTSLLYLGALKEAADLETSVGRPVLAKDDT
                     AKASALTSAIQINCWDQQRGLYADDPSKTIFSQHTNALAVLYDVAPKDAAKSILSKVT
                     QKSGIDAPDGILTTSYYFSWYLIHAYAHVGLGDRYLDQLRTWRDLAKLNYTTWPEERG
                     NTRSDTHAWSAHPTADLLGIVAGIQPASPGYGTVNVAPHPGMLNHFDAGAATPKGLVK
                     VSYRTTRERSSFTITLPTGLSGQFHWQGQSKLLRSGSQTFSVAKPNAE"
BASE COUNT          661 a          765 c          789 g          713 t
ORIGIN      
        1 ccccaactct gggggctatc cttccctatc gtaaactctt tattttcgcc ataccaatag
       61 tagctgtccc cgacagcgat aagtgatcca ccatgggctt ggatgggctt tccatttgtg
      121 tcaagccatt cggcgccagg ctgaataaag ctatagcgac gcttggaacc cgccatcgag
      181 gctttgggaa gcagagcgct ggccgatgtg gcgcaaaaaa taagaccagt agcgcctcgt
      241 gaaaattccc ttcgtgaaag tatcatatgt gaactccatt tttcgtgacg gtcaccgcgt
      301 catgttttga aattcgacat tttggaccga cgtcctgacg cgtcgatcta cgtcgaatct
      361 catttttctc tgacctgatt gattattttt ttgagcttgg aaaagtgtat ggcgacaacc
      421 atccattatc tctcaaccat gcctccaaga gcgccggcca ttggtcaagt tctggatctc
      481 ctttgccaag accagaacca tgtcttcctg taccaaaaac gtgcaactcc gctggcacat
      541 gtgattgcgt caacaagcgg tagaagccta tgcttcctcc cacaggaacc agttcgtcgg
      601 tcgtcgtatg atagatgaac attggtggcg tcttggcgtc gacatggtct tcgggcctat
      661 agcgagcgta ccaagctgaa tcacactcgg attcgattgc cattagcctg cagtatggaa
      721 ttgttttttc ggctggtgag aacatgttta atgcaggata gcctagcacg acgaagtttg
      781 ggcgagagtc agcacgatct acaacgtcgg tcgaattggg gtttcctgcc tcacctagcg
      841 tcgcttctaa cgctgccaaa tgaccgcctg cggagaaacc cattatgccg atacgatcgg
      901 gtgcaatacc atattctctt gccctgcttc gaaccaggcg tacggctcgc tgcgcatcta
      961 atagaggaac gggcatcgga ttattgcggc caacgcggta tttcagaacg aaggcagtca
     1021 cccctctcgc ggcaaaccaa tccgcgactt ctctaccctc caggcccgcc gctaccccga
     1081 gatacgctcc tcccggagcg atgataacag ccgttccggt cggcgcgccc tcagctggca
     1141 ttaccgtaag cgtaggttgg tcatccaggg atgaacttag tggggccggc ccgctccata
     1201 accggaaggt ccggatttca aggaagtcgt ccgcagcgcc attgagcggt cgctcaattt
     1261 tgatttcggg gacggtccgc agtttaacat ccgcaagttt gacctcatct gctgagatca
     1321 tcccctttga tagcctttgg acgacctgaa gagagatggt tcgcgatgcc agatatagag
     1381 ggttatttac cacctccggg acgaattttt gaaggatcgc gagtgtcgag ggattatcga
     1441 ctaactcgcc gagtggcgaa tttgcggaat atgttggggt ggcttcggtc acttcagtgc
     1501 tggattgacc tgctgttgca gaaccagatg acaacagcat caacatggct gctgcggccg
     1561 gaagggcgtg gatgagccga atgcgcgcta agcttcctag aaaattattc tgcatttggc
     1621 tttgccacgg aaaaagtctg cgaacccgat ctcaggagtt tggactgtcc ttgccaatgg
     1681 aactgccccg acaagcccgt cggcagcgtt attgtgaaac ttgaacgttc tctggtggtt
     1741 cgataactga ctttgacgag gccttttggc gtagctgcgc cagcatcgaa gtggttcagc
     1801 atcccaggat gtggagcaac attgactgtg ccgtaaccgg gggaagctgg ctggattccg
     1861 gcaacgatac ccaataggtc cgctgtgggg tgagcgctcc aggcgtgggt atcggatcgc
     1921 gtgttgcctc gctcttcagg ccaagtcgta tagtttagtt tagcaagatc gcgccaagtc
     1981 ctaagctgat cgagatagcg gtcaccgagg ccaacgtgag cataagcgtg gatcagatac
     2041 caggaaaaat aataactcgt ggttaggatg ccgtctggag catcgatccc ggacttttgg
     2101 gtgaccttgc ttaagatgga cttcgcggca tccttcgggg caacatcgta gagaaccgcc
     2161 aacgcgtttg tatgttggct gaaaatcgtt ttggaagggt cgtcggcgta caagccgcgt
     2221 tgttgatccc agcaattgat ttgaatcgcc gaggtcagag ctgacgcttt ggccgtatcg
     2281 tctttcgcga ggacaggcct gccaaccgat gtctcaagat ctgcggcttc tttcagcgcg
     2341 ccgaggtaga gaagtgaggt cagacaggat gttccagaag tcggatcgaa cgacggaaac
     2401 ttatcgcgag ccaagggggg atcgcctacc cagtcgacaa aattccattc cggatttcgt
     2461 ctcagtagcc cattgttgga aacatacggg gcgtaccaag tgagaatttt tcgcgctcca
     2521 ggtagattgc ggatgatcac cgccctatca ggccgctgca tccagtagtc gtgcatcatg
     2581 ccgatccaga gaaggccaaa cgtcggaatg atattgtttg aattgctggg gtaagcagac
     2641 tggatcatcc catcttgtct gacgctttca ccaaaggcat caatcgcctc cgcaggcaaa
     2701 cgggggtctc ctgaaacgct gagtgctatg aggctttcga ggcgggaatc ccccacatac
     2761 tgcagttgct cccagtatgc gctgtccatg aacgtctcat gtgcatcgat ctgcagggtg
     2821 cgccagccaa tatcccagat attgtcaagt tccggatcgc tgcttctgaa atagcccttc
     2881 gcctttaacg gataggcatc tctgcgcagc gtaaggttat ccagtctc
//
LOCUS       DATMTI010000289         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_138203, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000289 DATMTI010000000
VERSION     DATMTI010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138203"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="trmFO"
                     /locus_tag="VN039_04860"
     CDS             <1..724
                     /gene="trmFO"
                     /locus_tag="VN039_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HWV45351.1"
                     /translation="YNAFYDALMAAEKVQPKEFEKTSYFEACVPIEVLAERGRQTMQF
                     GPLKPVGLRDPRTGIEPAAVVQLRTENVHRTCYNLVGFQTKLTYPEQKRVFRMIPGLE
                     QAEFLRYGSLHRNTFINSPQLLLNTLQFKARGTLFFAGQLLGVEGYTESAAMGGLAGI
                     NAARALAGQPLITPPPTTAHGCLVSHITSSDPRHFQPMNTNFGLFPPLSNPPKDKEKK
                     RRALSQRALEDFDSWKMRSRLS"
     gene            697..1638
                     /gene="xerC"
                     /locus_tag="VN039_04865"
     CDS             697..1638
                     /gene="xerC"
                     /locus_tag="VN039_04865"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HWV45352.1"
                     /translation="MEDAIKAFVMHLQIERNASPETIRNYRSDLHQLTKFLQRTRHET
                     VPIHIDTITGDDIRTYLHRLDQEGEKASSLARKLACLRSFFRFLVREGQLRKNPTENL
                     RSPKLPKPLPRVLTKDDAAALMEFPTGQSPLSLRDRALLETMYSTGARVSEVVGINLE
                     DVNETDGIVCLRGKGRKERLVPIGDLALHAIRQYRRSLKAPVRSGHLSSPMFLNHRGG
                     RITTRSVARMVTRYSRRLPGGAVSPHALRHSYATHLLDEGADLRSIQEMLGHASLSTT
                     QKYTHLAMDQLLAVYDSAHPRARAAASPLSGKDRKSS"
     gene            1629..2171
                     /gene="hslV"
                     /locus_tag="VN039_04870"
     CDS             1629..2171
                     /gene="hslV"
                     /locus_tag="VN039_04870"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HWV45353.1"
                     /translation="MVMTIRSTTILCVRRDGRTAMGCDGQVTVGTTVMKHNAKKLRRL
                     HHDQVLAGFAGATADAFTLFEKFESKLEEYRGNLTRAAVELAKDWRTDRVLRRLEALL
                     AVAGREQSFIISGTGDVVEPEDGILAIGSGGPYALAAARGLLRHSQLEAPVIVTEAMT
                     IAGSIDIYTNQQIIVEELQG"
     gene            2181..3578
                     /gene="hslU"
                     /locus_tag="VN039_04875"
     CDS             2181..3578
                     /gene="hslU"
                     /locus_tag="VN039_04875"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HWV45354.1"
                     /translation="MNPLASDAEPLNLNSLTPHQIVEELNRYVIGQKDAKRMVAIALR
                     NRWRRQQLSPDLRDEVMPKNIIMIGPTGVGKTEIARRLAKLAEAPFIKVEASKFTEVG
                     YVGRDVESIIRDLTELAINMVKTQRLASVQQKAEQQGEERLLDLLLPPPPPRPGFVDS
                     TSEPAAQAPQDSHETTRSKLRLQLREGKLDERTVEMEVKERGLPIGVISNVGGLDDLE
                     SNLRDMLGGMFQGKKKKRLMKVPEALKHLTQEEAQKLIDIEDATREAITKVEQTGIVF
                     LDEIDKIAGRERTMGPDVSREGVQRDLLPIVEGCTVTTKHGPVVTDHILFIAAGAFHV
                     AKPSDLIPELQGRFPIRVELSALSKDDFVRILTEPKGALVRQYQALLATEGLAIEFSK
                     DGLEEIAEVAVQVNERTENIGARRLFTIMERLLEDISFEGPGWPDKRISITAAYVRDR
                     LKDIVKDQDLSRYIL"
BASE COUNT          827 a         1039 c         1050 g          714 t
ORIGIN      
        1 gtacaatgcc ttttacgatg ctttaatggc ggcggaaaag gtacaaccga aggaatttga
       61 gaagacgtct tattttgaag cctgcgtgcc gattgaagtg ctggcggaac gtggccgcca
      121 gaccatgcag tttgggcccc tcaagccggt tggcctgagg gatccacgca ccggaatcga
      181 gcccgccgct gtcgtccagt tgcgaacgga aaatgtccat cgcacctgtt acaacttggt
      241 cggctttcag acgaaactga cctatccgga gcagaagcgc gtgtttcgca tgatccccgg
      301 gctcgaacag gccgagttcc ttcggtatgg gagcctccat cgcaacacat tcattaattc
      361 gcctcagctc ctcctcaata cgctgcagtt caaagctcgc ggcacattgt ttttcgccgg
      421 gcagctcctc ggcgtcgaag gctataccga atcggctgcc atgggaggat tggccggcat
      481 caatgccgcg cgggccttgg ctggacaacc actgatcaca cctccgccta cgaccgcgca
      541 cggctgctta gtttctcata tcacctcgtc ggatcctcgc catttccagc ccatgaacac
      601 taatttcggc ctgttccctc ccttgtcgaa ccctcccaaa gacaaagaga agaagcgtcg
      661 cgcgttgagc cagcgcgcgc tggaggattt tgactcatgg aagatgcgat caaggctttc
      721 gtaatgcacc tccagatcga acgcaacgcg tcacccgaga cgattcgcaa ttatcgatcc
      781 gaccttcatc agttgacgaa gtttcttcaa cgaaccaggc atgaaaccgt acccattcac
      841 atcgatacga tcaccggcga cgatattcgt acctacctac acaggttgga tcaggaaggg
      901 gagaaagctt cctctttagc cagaaagttg gcttgcctgc gaagcttttt ccggttcctc
      961 gttcgtgagg gacagcttcg taagaacccc acggagaacc tgaggagtcc caagctacca
     1021 aagccactcc cccgagtatt gacaaaggac gatgccgcgg cactcatgga gtttccgacc
     1081 gggcaatcgc ccctgtcctt gcgcgatcgt gccctgctgg aaacgatgta ctcgaccggg
     1141 gctcgggtga gtgaggtcgt ggggatcaat ctcgaggacg tgaacgagac ggacggaatc
     1201 gtatgtttga gagggaaggg tcgcaaggaa cggctggtgc cgatcgggga tctggcgctc
     1261 cacgcgatcc ggcagtatcg caggtcgttg aaagcgccgg ttcgcagcgg ccacctgtcg
     1321 tctccgatgt ttttgaatca tcgtgggggt aggatcacca cgaggagcgt cgctcgaatg
     1381 gtcactcgat attccagacg tctcccgggt ggagcggtca gtccccatgc cttgcgacac
     1441 tcctatgcga ctcatctgct cgacgaaggt gcagatctcc ggtcgataca ggaaatgctc
     1501 ggccacgcct cgctgagcac gacacaaaag tatacgcatc tggcaatgga tcaactcctt
     1561 gccgtgtatg atagcgccca ccctcgagca cgggcggcgg caagcccctt gtcaggaaag
     1621 gaccggaaat cgtcatgacc attcgatcga ccactatcct ttgtgtccgg cgcgacggcc
     1681 ggactgccat gggttgcgac ggccaagtga ccgtcggaac cacggtgatg aaacacaacg
     1741 ccaaaaagct gcgacgcttg catcacgacc aggtgctggc cggatttgcc ggggccaccg
     1801 cggatgcctt cactctgttc gagaaattcg agagcaagtt ggaggagtat cgaggcaacc
     1861 tcaccagggc agcggtggag ctcgcgaaag actggcgaac cgaccgtgtg ctcagacggt
     1921 tggaggcctt gcttgccgtc gccggccggg agcagtcctt catcatttca ggaaccggcg
     1981 atgtcgtgga gccggaagac ggcattttgg ccatcggttc gggtggaccc tatgccttgg
     2041 cggcagcccg tgggctctta cgccattccc aacttgaggc tcccgtgatc gtcaccgaag
     2101 ccatgaccat cgcaggttct attgacatct acaccaacca acagattatt gttgaagaac
     2161 ttcaaggata acgcactatc atgaatcctc tcgcaagcga tgccgaacca ctgaatctca
     2221 atagcctgac cccacatcag atcgtggagg aactgaatcg ttacgtcatc gggcaaaagg
     2281 atgcgaaacg aatggtcgcc atcgcccttc gcaaccggtg gcgccgccag cagctgtcgc
     2341 ccgatctccg ggatgaagtc atgccgaaga acatcatcat gatcgggccc accggcgtgg
     2401 gcaaaacgga gattgcccga cggcttgcca agctggccga agcccccttc atcaaggtcg
     2461 aagcgtcgaa gttcaccgag gtcggctacg tcggacgtga tgtggaatcg atcattcgcg
     2521 acttgaccga actggccatc aatatggtca agacacaacg gctggcgtcc gtgcagcaaa
     2581 aggcggaaca acaaggcgag gaacgattgc tcgatctgct cttgccgccg cctccccctc
     2641 gaccgggctt tgtggacagc acgagcgagc cggccgctca agcccctcag gattcccacg
     2701 aaacgacgag atcgaaactg cgcctgcagt tgcgggaagg gaagctggat gaacgaacgg
     2761 ttgagatgga ggtgaaggag cgaggtctcc ccatcggtgt gatttccaac gtgggcggcc
     2821 ttgacgatct cgaaagcaat ctccgcgata tgctgggcgg catgttccaa ggcaagaaaa
     2881 agaaacggct gatgaaagtg ccggaggcgc tcaagcactt gacgcaggaa gaagcccaga
     2941 aactgattga tatcgaggat gcgacgaggg aagccatcac caaggtggag cagaccggga
     3001 ttgtgttcct tgatgagatc gacaagatcg ccggccgcga gcgcaccatg ggacccgatg
     3061 tgtcgcgaga aggtgtgcaa cgcgatctcc tccccatcgt ggaaggctgc accgtcacca
     3121 ccaaacatgg cccggtcgtg accgatcaca tcctcttcat cgctgctggc gcttttcatg
     3181 tggcgaagcc gtccgatttg attccggagc tccaggggcg atttccgatc cgcgttgagc
     3241 tcagcgcgtt atcaaaagac gatttcgtcc gtatcctcac ggaaccgaaa ggggcgttgg
     3301 tccggcagta tcaggccttg ctggcgacgg aaggcctcgc catcgagttc tccaaagacg
     3361 gtctggagga gatcgccgag gtggcggtgc aggtgaatga acggaccgag aacatcggag
     3421 cgcgtcgcct cttcaccatc atggagcggt tgcttgagga catttctttc gagggacccg
     3481 gctggcccga caaacggatc agcatcaccg ctgcctatgt ccgggacaga ctgaaagata
     3541 tcgtgaagga ccaggacctg agccggtata tcctctaagg gcccggtgtt gatcgtcgaa
     3601 agttcggcat gaataaactg atcaagaagg
//
LOCUS       DATMTI010000290         1989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_604937, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000290 DATMTI010000000
VERSION     DATMTI010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604937"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /gene="acs"
                     /locus_tag="VN039_04880"
     CDS             <1..1751
                     /gene="acs"
                     /locus_tag="VN039_04880"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWV45355.1"
                     /translation="AAEELEWFSPWEKVLEWNYPWAKWFVGARCNITYNCLDRHIKIW
                     RKNKVALVWIGENDQERIFTYAELHRQVNRCANALKKLGLQREDRVTIYLPKIPEQVV
                     AMLACARIGVIHSVVYSGFSASALASRINDAESKVVITADVGFDRGKAIQLKSVVDEA
                     VKTCPTIDHVVVVRRQIQGPPLSAPKEIDWKEWVENERPVCEAEQLDAEAPLYILYTS
                     GTTGRPKGVVHVHGGYMVGTYTTTKYVFDLKDDDVYFCVADPGWVTGHSYIVYGPLLN
                     GATILTAEGKPDYPNPGRWWDLIERYGVSIFYTTPTAIRLLMRYGEDWPKKFDLSTLR
                     ILGSVGEPINPEAWEWFHRVTGENKPIMDTWWQTETGSILITPLPTVPLKPGSATRPF
                     LGIEADVVDREGNSLPAHAGGFAVIKKPWPAMMRTIYKDPDRYKAYWSTIPNCYTAGD
                     VCHKDADGYFWFMGRADDVIKVAGNRLGTAEVESALVSHPAVAEAAVIGKPHKTVGES
                     IKAFIILKQGEQESPALIKSIKDQVLKELGKIGVPAEIDIVASLPKTRSGKIMRRVLK
                     AKELGQDPGDISTIEE"
     gene            1806..>1989
                     /locus_tag="VN039_04885"
     CDS             1806..>1989
                     /locus_tag="VN039_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45356.1"
                     /translation="MEKLTIFAAAISIGIMTLSLQIIDPGLTSAADQEPKAKPHSKTV
                     KPKTVKPKAVKPKTVKP"
BASE COUNT          500 a          570 c          536 g          383 t
ORIGIN      
        1 atgccgccga agagttggag tggttctctc cttgggagaa ggtcctggag tggaactatc
       61 catgggcgaa atggtttgtc ggcgcacgat gcaatatcac ctacaactgc ctggaccgac
      121 acatcaagat ctggcgcaag aacaaagtcg cgctggtctg gatcggcgaa aacgatcagg
      181 aacgaatctt cacctatgct gaactccacc gacaagtgaa ccgctgcgcc aacgccctca
      241 aaaagcttgg cctccagcga gaggatcgtg tcaccatcta tctgcccaaa attcccgagc
      301 aagttgtcgc tatgctggct tgcgccagaa tcggcgtgat tcacagcgtc gtctattccg
      361 gtttcagcgc ctctgccctc gccagccgaa tcaacgatgc agagtccaaa gttgtgatca
      421 ccgccgatgt gggcttcgac cgaggcaagg ctatccaatt gaagtccgtc gtggacgaag
      481 ccgtcaagac ctgtccgacg atcgaccatg tggttgtcgt acgccgccag atccaaggcc
      541 cgcccctttc cgccccaaag gaaattgact ggaaggagtg ggtcgagaac gagcgcccgg
      601 tttgcgaagc agaacagctg gatgccgaag ccccgctcta tattctctat acgtccggca
      661 cgaccggtcg gccgaagggc gtcgtccatg tccatggagg gtacatggtc ggcacctata
      721 cgacgacgaa gtatgtcttc gatctgaaag atgacgacgt gtacttctgc gtggctgatc
      781 cggggtgggt cacaggccac agctatatcg tctacggtcc tctcctcaac ggcgcaacga
      841 ttctgaccgc ggaagggaaa ccggattatc cgaatccggg acgctggtgg gatctcatcg
      901 aacggtacgg ggtctcgatt ttctatacga ccccgaccgc gatcagactg ctcatgcgct
      961 acggcgaaga ctggccgaag aaattcgatc tctcgacgct ccgtatcctc gggagcgtcg
     1021 gagagccgat caacccggaa gcctgggaat ggttccatcg tgtgacaggc gaaaacaaac
     1081 ccatcatgga cacctggtgg caaacagaaa cgggatcgat cttgatcact ccgcttccca
     1141 ccgtgccgct gaagcccggc tcggccacac gcccatttct tggtattgaa gccgatgtcg
     1201 tcgatcgtga aggtaacagt ctccccgcac acgccggcgg ctttgccgtc atcaaaaaac
     1261 catggccggc catgatgcgc accatttaca aggatcctga tcggtataag gcctactggt
     1321 ccaccatccc caattgctat accgccggcg acgtctgcca taaggatgca gacggctact
     1381 tctggttcat gggtcgagcg gatgacgtga tcaaagtcgc cggcaatcgg ctcggcactg
     1441 ctgaagtgga aagcgcattg gtcagtcacc ccgccgtcgc ggaggctgcc gtgatcggaa
     1501 aaccgcacaa gacggtcggc gaatccatta aggccttcat cattttgaaa cagggagagc
     1561 aggaaagtcc ggcgctgatc aagtcgatca aggatcaggt ccttaaagaa ttaggcaaga
     1621 tcggcgtccc cgctgaaatc gatatcgtcg cttccctccc caagactcga tctggaaaaa
     1681 tcatgcgccg cgtcctcaaa gccaaagagc tggggcaaga tccaggagat atctcgacaa
     1741 tcgaagaata gcgtacagag aagttaaact cgatatgggc ctaaccgagc gaagggaacg
     1801 acataatgga gaaactaacg attttcgcgg cggcaatttc gattgggatc atgaccctgt
     1861 ccttacagat aatcgatcca ggactcacgt ctgcagcgga tcaggaacca aaggccaaac
     1921 cacactccaa gacggtcaaa ccaaaaaccg tcaagcccaa ggctgtcaaa ccaaagaccg
     1981 tcaaaccca
//
LOCUS       DATMTI010000291         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_138213, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000291 DATMTI010000000
VERSION     DATMTI010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138213"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VN039_04890"
     CDS             <1..963
                     /locus_tag="VN039_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HWV45357.1"
                     /translation="AEGYLLTGRHMFFASYEAFLHIIDSMVNQHAKWLEKCKTEVKWR
                     ASISSLNLLITSTVWRQDHNGFTHQDPGFLDVVTNKSSKVTRIYLPPDANCLLSVADH
                     CLRSVDYVNVIVADKQLHLQYLDMAAAVRHCEKGIGIWPWASTDQGSAPDVVMACAGD
                     VVTMEALAATAILRERFPELKIRFINVVDLFKLEPITDHPHGLTNRDFDGLFTRDKPV
                     IFNFHGYPALIHKLAYRANHDLWHVHGYNEKGSIDTPLTLAILNEVDRFSLAIDVIDR
                     VPRLQESGVHVKNWLQDQILEHLAYVHEQGIDPPEIRNWKWPYT"
     gene            1014..2909
                     /locus_tag="VN039_04895"
     CDS             1014..2909
                     /locus_tag="VN039_04895"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWV45358.1"
                     /translation="MTVTTPEQTTLDILQDKATRLRIDSVRSTTAAGSGHPTSCASAA
                     EIMSVLFYSVLRYDPRDPHNRDSDVFVLSKGHAAPILYAAWAEAGAFPREKLLTLRQI
                     DSDLEGHPTPRLPFIDVATGSLGQGLSVSLGIALHAKRFGPSDQRVYVLMGDGESAEG
                     SVWEAAQWATLHDLSNLCATIDINRLGQSQPTMLQRHLDIYKARWEAFGWQALSVDGH
                     SIPDLQAAYETARLTTDRPTIVLARTIKGKGFVDIEDREHWHGKALDYDTAAKVVVDL
                     KQQLTGAGTEWAPHLPPARPVSTTIMRDSRVESAEKSPYVIGSKEVATRKGFADSLAA
                     LARTDPRIVVLDGDVKNSTYTEEFEKVAPDRFFQGYIAEQNIVGMTMGLAARGKVPVA
                     ALFACFVTRAYDFIRLAAISKLNIKLVGTHAGLSIGEDGPTQMGLEDLAMTCAEPTFT
                     VLYPADATSAWQATRLITEHSGPCYLRLGRPNSPILYGPDERFAIGQCKVLRKSGQDQ
                     VLVIAGGITVFEALAAYDQLQQEGISIRVIDLFSVRPIDRDELIASARATGGIIITVE
                     DHYEHGGLGDAVLSALAGERIQAYKLAVRDIPHSGQPKELIEKFGISAARIVTTVKSA
                     LRSPACS"
     gene            3074..3562
                     /locus_tag="VN039_04900"
     CDS             3074..3562
                     /locus_tag="VN039_04900"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HWV45359.1"
                     /translation="MTDPSSELPRMLTRIGVASDHGGFELKRYLVEMLEAAGYGVIDF
                     GDRHLTPDDDYPDFVIPMARAVVRGEVDRGVAICGSGVGACVAANKVPGVRACLIHDM
                     FSAHQGVEDDDLNIICLGGLVVGRALAWELVKTFLAGRFRDGERYRRRLAKVAELESK
                     EA"
     gene            3565..>3939
                     /locus_tag="VN039_04905"
     CDS             3565..>3939
                     /locus_tag="VN039_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HWV45360.1"
                     /translation="MKTNRLKKLGTFGQSIWLDYIRRDLIAGGQLRRLIDEDGLRGMT
                     SNPSIFEKAIVESHEYDDDIRAMALEGKEVITIYEALSQHDVQCAADEFRPLYDKTDG
                     RDGYVSLEVNPHLAHDAKGTIEE"
BASE COUNT          840 a         1138 c         1189 g          772 t
ORIGIN      
        1 gcggagggct acctgttgac cggccgccac atgttcttcg cttcctatga agcgttcctt
       61 cacatcatcg attccatggt caaccagcat gccaaatggc tggaaaaatg taaaaccgag
      121 gtcaaatggc gagcgtccat ctcatccttg aatctgttga tcacctccac cgtctggcgt
      181 caggaccaca atggcttcac ccatcaagac ccgggattcc ttgatgtggt caccaacaag
      241 agttcgaagg tgacccgcat ctatctgcct cccgacgcga actgcctgtt gagcgtggcg
      301 gatcactgtt tgcgtagcgt cgattatgtg aatgtgatcg tagcggacaa gcagctgcat
      361 ctgcagtatt tggacatggc ggctgccgtg aggcattgcg agaagggcat tgggatttgg
      421 ccctgggcca gcacggatca gggcagcgca ccggacgtgg tgatggcatg cgcaggcgat
      481 gtcgtcacca tggaagcgct ggccgcgaca gccatcttac gcgagcggtt cccggagctg
      541 aagattcggt tcatcaacgt ggtcgatctg ttcaaactcg agccgatcac ggaccaccct
      601 catgggttga cgaatcgcga cttcgacggc ttgtttacga gggacaaacc cgtcatcttc
      661 aacttccacg gatacccggc gctgattcac aagctggcct accgggcaaa ccacgacctc
      721 tggcacgtcc acggctacaa cgaaaaaggg agtatcgaca cgccgctcac gctggccatc
      781 ctcaacgagg tggaccggtt cagtttggcg atcgacgtca tcgatcgagt cccgcggcta
      841 caagagtccg gagttcacgt caagaactgg ctccaggatc agatcctgga gcatctcgca
      901 tatgtccacg aacaaggcat cgatcctccg gagatccgaa actggaaatg gccatacacg
      961 taagaaagcg ccgcgatcac acagattctt ttcataccga aaggagtgga ctcatgactg
     1021 tcacaacacc ggaacagacg acattggaca tcctgcagga caaggcgacg cgcttgcgga
     1081 tcgacagcgt ccgatcgacg accgcagccg gcagcggaca cccgacgagc tgcgcctcgg
     1141 cggcggagat catgtccgtg ctgttctatt ccgtgctgcg atatgatccc cgcgatccgc
     1201 acaaccgtga cagcgatgtc ttcgtgctct cgaagggcca cgccgcgccg atcctctatg
     1261 cggcttgggc ggaagccgga gcctttccgc gcgagaaact gctgacgttg cgccaaatcg
     1321 actccgatct ggagggccac cctactcccc ggttaccgtt tatcgatgtc gcgaccggtt
     1381 cgctgggtca gggattatcg gtctctctgg ggattgccct ccatgcgaaa cgattcgggc
     1441 catccgacca acgggtgtat gtgttgatgg gcgacggcga atcggccgaa ggctcggtct
     1501 gggaggccgc gcaatgggcc actctccacg acctgagcaa tctctgcgcg accatcgata
     1561 tcaatcgtct gggtcagagc cagccgacca tgctgcagcg ccatctggac atctacaagg
     1621 ctcgctggga ggccttcggc tggcaggcgt tgtccgtgga tgggcacagt attcccgatc
     1681 tgcaggcggc ttacgaaacc gctcgtctga ccaccgatcg tccaacgatt gtgttggcga
     1741 ggacgatcaa gggcaaagga tttgtagaca tcgaggatcg tgaacactgg catggcaagg
     1801 ccctggatta cgataccgca gcgaaggtcg tcgtcgatct gaaacagcag ctcacgggcg
     1861 ccggcacaga atgggcgccg catctgcctc cggctcggcc cgtctccaca accatcatga
     1921 gggacagtcg cgtcgagtcg gcagagaagt cgccgtatgt catcggaagc aaggaagttg
     1981 ccacgagaaa aggctttgcc gacagtttgg cagcgttggc gcgcaccgat cctcgcattg
     2041 tggtgctcga cggtgacgtc aagaactcca cctacaccga agaattcgag aaggttgcgc
     2101 cggaccgctt ctttcaggga tacattgcgg aacagaacat cgtgggaatg accatgggat
     2161 tggcggcgcg aggcaaggtg cccgtcgcgg ccctctttgc ttgcttcgtc acccgggcct
     2221 acgatttcat ccgcttggcc gccatcagca agctcaatat caagctggtc ggcacgcacg
     2281 ccggcctgtc catcggggag gatgggccca cccagatggg gctcgaagat ctggccatga
     2341 cgtgcgcgga gccgaccttc acagtgctct atcccgccga tgccaccagc gcctggcaag
     2401 ccaccaggct gatcacggag cactccggtc cctgctatct ccggctggga cgaccgaact
     2461 cgccgattct ctacgggccg gacgaacggt ttgcgatcgg tcaatgcaaa gtgctccgaa
     2521 agagcggcca ggaccaggta ctcgtcattg ccggcgggat cacggtcttt gaagcgttgg
     2581 cggcgtacga ccaactccaa caggagggca tttccatccg cgtcatcgat ctgttcagcg
     2641 tacgaccgat cgatcgagac gagctgatcg cctccgctcg cgccacgggc gggatcatca
     2701 tcacggtcga agatcactat gagcatggag gactcggcga tgccgtgctc tcggccctcg
     2761 cgggagaacg tatccaggcg tataagttgg ccgtgcgtga cattccgcac agcggacaac
     2821 ccaaggagct gatcgagaag ttcggcattt ctgccgcgcg catcgtgacc acggtcaagt
     2881 ccgccttacg gtcgcccgca tgctcgtgag catccgatga ggtcgcggag catgagatga
     2941 tcacactgaa tcgcgtctat gcccctgtga cgtaggctga tggtgtgcgc gttcgtaacg
     3001 gagccgagtc attaatgagc ggtagctacg caaggtgtct gcgcatgagc gcaagccgtg
     3061 cagaaacgga cctatgactg atccatcgtc ggagttgcca cggatgctca cccgtattgg
     3121 ggtcgcctcc gaccatggcg ggttcgagtt gaaacgctac ctggtcgaga tgttggaggc
     3181 ggcgggctac ggcgtgatcg acttcggcga tcgccatctg accccggacg atgattatcc
     3241 agactttgtt attcccatgg ctcgcgccgt cgttcgcgga gaggtggatc gcggcgtggc
     3301 catctgcggc agcggggtgg gagcgtgcgt ggccgccaac aaggtgccgg gcgtgcgagc
     3361 gtgcctgatc catgacatgt tttctgcaca ccaaggcgtc gaagacgatg acctgaacat
     3421 tatctgcttg ggcggcctcg tggtcggccg ggcgctcgca tgggaactgg tcaagacatt
     3481 tctcgcaggg cgatttcgtg acggggagcg ttatcgccgc cgcttggcga aagtggcaga
     3541 actggaaagc aaggaggcat gagtatgaag accaatcgtt tgaagaaatt aggaacgttc
     3601 ggtcagtcca tctggttgga ctacatccga cgcgatctga tcgccggtgg acagcttcgg
     3661 cgtctgattg acgaggatgg cctacgggga atgacctcga atccctccat ctttgagaag
     3721 gcgatcgtag agagccatga gtatgacgat gacattcggg ccatggctct tgagggaaaa
     3781 gaagtgatca ccatctacga ggccctcagc caacacgacg tgcaatgcgc cgccgacgag
     3841 ttccgaccgc tgtatgacaa gaccgatggc cgcgatggat atgtcagttt agaagtgaat
     3901 ccccatctgg cgcacgacgc caaaggcacg atagaagaa
//
LOCUS       DATMTI010000292         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_181116, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000292 DATMTI010000000
VERSION     DATMTI010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181116"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2128
                     /gene="mgtA"
                     /locus_tag="VN039_04910"
     CDS             <1..>2128
                     /gene="mgtA"
                     /locus_tag="VN039_04910"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HWV45361.1"
                     /translation="VDDGSVEISVHDLVPGDIVSLRAGSLVPADVRLIEVKDLHVNQS
                     ALTGESFPVGKSAQPTGSQNEPVASDPIQLENLCFMGTSVVSGLGKAVVLATGDRTYF
                     GNLAVTITTEEFATSFEKGLTSLSWLLIRFILGMVVIILLINGLTKGDWWLSFIFALS
                     VGVGMTPELLPMIVTTNLSRGAIEMSGKKVIVKRLESIQNLGAVDVLCMDKTGTLTQN
                     KVILYKHLDLQGKESIPVLKYAYLNSHFESGLKNLLDDAILEHAEQEDSLKPDPAYHK
                     IDEIPFDFERRRLSVIIADNQKQLLICKGAVEEVLSVCSQYEEQGKVSPIDDAIRQQA
                     RKQMESLNQDGFRVIAIAYKVCEEKQSSWKAQDENNLVLLGFIAFLDPPKDTTAKALT
                     ELIKLGIEPKILTGDSELVTKKICNVVKLPIKGVMLGKDVESLNDEELSQHVDQCTIF
                     ARLNPGQKERIIHALRKADHVVGFLGDGINDSPAMNAADIGLSVNNAVDIAKESADMI
                     LMEESLMVLNDGVIAGRKVFGNIIKYIRMTTSSNFGNVFSMVGASFLLPFLPMKPIQI
                     LTQNLLYDFSQLAIPFDHVDEEFLQKPHRWQVANVERFMLTFGPVSSLFDYTLFGVMW
                     FVFQANSPSHQAVFQTGWFIEGLLSQTLIVHLIRTRKIPFIQSWAAPQLIALTLTIMA
                     IGIYLPLSPLARFFGFVHPPQHYFLWL"
BASE COUNT          486 a          596 c          567 g          479 t
ORIGIN      
        1 agtggatgac ggcagtgttg aaatttccgt gcatgatctc gtgccgggcg atatcgtcag
       61 cctgcgcgca gggtcattgg taccagcgga tgtgcgcctg attgaggtca aggatctgca
      121 cgtcaaccag tccgccttga ccggagaatc ctttccagtt ggaaaatccg cgcaacccac
      181 cggctcgcaa aatgaaccgg tagcttccga tcccatccaa ctggaaaacc tgtgctttat
      241 gggcaccagc gtagttagcg gcctgggtaa agccgtcgtt ctggccactg gcgaccgaac
      301 ctacttcggc aatctggccg tcaccatcac cacggaagaa ttcgccacca gctttgagaa
      361 agggcttacc tcgctcagct ggctattaat ccgcttcatt ttgggtatgg tggtcatcat
      421 tttactgatc aacgggctga ccaagggcga ctggtggttg tcctttatct ttgcgctatc
      481 ggtcggggtg gggatgacgc cggaactgct gcccatgatt gtcaccacca acctctcacg
      541 gggggccatt gagatgtccg gcaagaaggt gatcgtgaag cgtctggaat ccattcagaa
      601 cctgggcgcg gtggacgtgc tgtgcatgga taaaaccggc acgctgaccc agaacaaggt
      661 catcctgtat aagcacctgg acttgcaggg caaggagagc atcccggtct tgaagtacgc
      721 ctacctgaac agccattttg aatcggggct gaagaacctg ctggacgacg ccattctgga
      781 acatgccgag caggaggatt ccctcaagcc cgatccggcc tatcacaaga tcgatgaaat
      841 tcccttcgac tttgagcggc gacggctttc cgtgatcatc gcggataacc agaagcagtt
      901 gctgatctgt aaaggcgctg tggaggaagt gctttcggtt tgttcgcagt atgaggaaca
      961 ggggaaagtt tccccgattg atgacgcgat acggcagcaa gcgcgaaagc aaatggagtc
     1021 gctcaaccag gacggctttc gggtcatcgc gattgcttac aaagtctgcg aggagaaaca
     1081 atccagctgg aaggcgcaag acgaaaacaa cctcgtcctg ctcggtttca tcgcgttcct
     1141 ggatcccccc aaggacacca cggccaaagc gctgaccgaa ctgatcaagc tgggcattga
     1201 acccaaaatt ctcaccgggg atagcgagct ggtcactaag aagatctgta atgtggtcaa
     1261 gcttcccatc aagggcgtca tgctgggaaa ggacgtagag agcctgaacg acgaggaact
     1321 gagccagcat gtggatcaat gcaccatctt tgcccgcctg aatcccgggc agaaagaacg
     1381 catcatccac gccctacgca aagccgatca cgtggttggt ttcctgggcg acggcattaa
     1441 cgattctccc gccatgaatg ccgccgatat cggcctgtcc gtcaacaatg cggtggacat
     1501 cgccaaggaa tccgccgaca tgatcctgat ggaagagagc ctcatggtgt tgaacgatgg
     1561 cgtgattgca ggccgaaaag tgttcggcaa tatcatcaaa tacatccgca tgaccaccag
     1621 ttccaacttc ggtaacgtct tcagcatggt gggcgccagt tttctgctgc ctttcctgcc
     1681 gatgaaaccg atccagatcc tgacccaaaa cctgctgtat gatttctccc agctagccat
     1741 tccctttgat cacgtggatg aggaatttct gcaaaaaccg caccggtggc aagtcgccaa
     1801 tgtggaacgc ttcatgctca cctttggccc cgtaagttcc ttgttcgatt acaccttatt
     1861 cggggtgatg tggtttgtct ttcaggccaa ttcgccaagc catcaggctg tgttccagac
     1921 cggctggttt attgaagggc tcctgtccca gacgctgatc gtgcacctga tccgcacccg
     1981 gaaaatcccg ttcatccaaa gttgggccgc cccgcaactc atcgccctca cgctcaccat
     2041 catggcgatt ggtatctatt taccactatc cccgctggcg cgcttctttg gctttgtgca
     2101 ccctccccag cattacttcc tttggctg
//
LOCUS       DATMTI010000293         5428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_265302, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000293 DATMTI010000000
VERSION     DATMTI010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265302"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..424)
                     /locus_tag="VN039_04915"
     CDS             complement(194..424)
                     /locus_tag="VN039_04915"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HWV45362.1"
                     /translation="MPKKTQSVSRSTRPGAGVESRGNGNGITNRPDENSHGIHARISE
                     RAYALYEEHGRRDGHALEDWLAAEQHVLNQAV"
     gene            complement(505..912)
                     /locus_tag="VN039_04920"
     CDS             complement(505..912)
                     /locus_tag="VN039_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45363.1"
                     /translation="MMTSRQHGERTRVMIVEQDLEFGLKLADWLATHGYHPAFISTVN
                     AAIDGLAGFRPQAVFVGLGCSAPAAHVNMVEILLLIQTICPSVPVIMMADQIHEDLTQ
                     VVFRQGVRRLLVKPVEFSHIGEVLRSELRTATV"
     gene            complement(958..1245)
                     /locus_tag="VN039_04925"
     CDS             complement(958..1245)
                     /locus_tag="VN039_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45364.1"
                     /translation="MAETAPSVDAVMCREITGVGSCTFDELVHRLPTYSWAQVFSAVD
                     RLSRQGTLTVSRTRRFGYVVSMGPAPLIPESSQDRECQAVRPQVIRYGAAA"
     gene            complement(1490..2131)
                     /locus_tag="VN039_04930"
     CDS             complement(1490..2131)
                     /locus_tag="VN039_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV45365.1"
                     /translation="MLKVLVVDDYPLFRRGVKDLVNEGFQGAKIGEAGNAFEMLELLR
                     RRPWDVAVLDINMPGMNGLDALKQVKQEFPKLPVLILSMYPEEQYAIRMVKAGADGYL
                     TKSSAPEELVKAITKVRSGGKYVSPSLAEALIVTIRPGAAKEPHQWLSDREYQVLCFI
                     GSGKTVGEIAGRMNLSVSTISTYRARILEKMGMKTNAELTRYVLQHGLAEGAC"
     gene            complement(2141..5002)
                     /locus_tag="VN039_04935"
     CDS             complement(2141..5002)
                     /locus_tag="VN039_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV45366.1"
                     /translation="MKDKKRRRGSPSAVPKLRAQRSKPSSTPVSTVGVGESGGGEGAT
                     AQDRSAQELRKSETRFHNPADTAPAMLWVTEPDGSASFISRGWYEFTGQTEQEGLDQN
                     GFGRLDAVHPEDRERSGRMFLEANKARRPFSLDYRLRRHDGEYRRAIDAGNPRFGPNG
                     EFLGYIGSVMDITERKLAEVALRESEEKYRTVFTSIDEGFCVVEMLLDEQGKPHDYLL
                     LEINPMFEQLTGLRNAVGRTARELVPGLEEFWVEIYGRVALTGEPVRFEHYAEPMNRW
                     FNVHASRVGDETSCRVAIVFTNITERKQAEALLAAEKKVLELIATGTAMPEVLDTMMR
                     ETEAQSTDGMLCSVLLADEKGQRLLHGAAPSLPEAYNTAVHGLVIGPKAGSCGTAAFK
                     HKPVFVDDIAGDPLWNDFKHLAATHGLGACHSTPIVSSQGRLLGTIAMYYRRPHNPGA
                     HDRQLIERATHLAGIAIERRQAEEALRLAKFSVDRAADAVYWIDPQAKVLDVNEAASR
                     MLGYSKDELCAMTVHDLNPDFQTDMWPGFWAETRKRGTMVIETFHKAKNGRMIPIEVS
                     VNFLSYEGKEYHCASVRDITERKRVEAALRKSEERYHSIFENAVDGIFQTTADGKYVT
                     VNPALARIHGYDSPDDMIKTITDIASQLYVDPGRRDVFLSLMQAQEEVTGFESLVYRK
                     DGSFIWISESARTLRDQAGAVVGYEGTVEDITERKRAERQLRATLDQVRTLSGRLSTL
                     QEKERTRIARELHDELGVRLTCLKIDLSRMMSVVGNRVRADLRAKLNDGVRSMVGQVD
                     STIAAVQRLVTQLRPALLDDLGLVAALEWQSQDFQKRTGISCTCVTSADDIAMEPERA
                     TAVFRICQEALTNTARHAQATTVIITVTSQPDALQMTVTDNGVGIPDTKASDPQSLGL
                     LGMQERAAQCGGTLTIEGDPEKGTTVTLCLPIGGGQPAS"
BASE COUNT         1067 a         1648 c         1509 g         1204 t
ORIGIN      
        1 ggtggccaca agtcctcggc tcgtatgcag tgccgaacgc gcgtgccctt cttcatctcc
       61 attcggcatt cccactccgc gaatgaacac tgggttgccg gacttgtcga cagaatgctg
      121 aatcacgaac tgtccgttcc cattcatttc atggcaggct tgaagatgcc ttggtggatg
      181 gtccaacatc ctgctacaca gcttgattga gtacgtgttg ttcggccgcc aaccagtctt
      241 caagagcatg gccgtccctt cggccatgct cctcatacag agcataggcg cgttccgaga
      301 tgcgcgcatg gataccgtgg gaattttcat caggccggtt ggtaatgccg ttcccgttgc
      361 cccgtgattc gactcctgcg ccaggtcgtg tggagcgtga gaccgactga gttttcttgg
      421 gcatgacgaa ttctcctcct aatttataac aacgcttaca gtaatcttgc gcttctctac
      481 gggttgggcg acgatttcac cgatttatac cgttgctgta cgcagttccg accgaagcac
      541 ctcgccgatg tgcgagaatt ccaccggctt gaccagcaaa cggcgaactc cttggcgaaa
      601 cacaacttgc gtcaggtctt cgtggatttg atccgccatc atgatcaccg gaacggaggg
      661 acaaatggtc tggatcagga ggagtatctc aaccatgttg acatgggctg cgggcgcaga
      721 acagccgagg ccgacaaaga ctgcctgcgg cctgaaaccg gccaagccgt cgatcgcggc
      781 attgactgta ctgatgaatg ccggatgata gccgtgagtt gcgagccaat cggcaagttt
      841 caatccaaat tccaagtcct gttccacaat catcaccctg gttctctcac cgtgctgccg
      901 gctcgtcatc atccgatctc ctttagcttc tgccttagcc tcatgctgct tcacggttca
      961 cgcggcggcg ccgtatcgga tcacctgcgg tcgtaccgct tggcactccc ggtcttgcga
     1021 agactcggga atgagaggag ccggtcccat agataccacg tagccgaagc ggcgcgtacg
     1081 gctgaccgtg agggttcctt gccggctgag ccgatccacc gccgaaaaca cttgcgccca
     1141 ggaataggtc ggcaaccggt gaaccaactc atcgaaggta caggacccaa cgccggtgat
     1201 ttctcggcac atgactgcgt caacactcgg ggccgtttca gccataacat tctcctttgg
     1261 tgaggagagg acaacaggcg atctattgct caaggcgtat ggctggatgt atagccgagt
     1321 ttgatgagtg accccatcga caaaaacggg attttcttat cgaatcggca actacggcac
     1381 tgtcgtggtt cttacgacag aggagagaag aactgttgag atgagagtac tgagtgttca
     1441 gcgcggaagc cggagagggc agagatcagg gaccaaaggg tggcagaact cagcatgctc
     1501 cctccgcgag tccatgttgc agcacgtatc gggtgagctc cgcgttggtc ttcatgccca
     1561 tcttttcgag aatccgtgcg cggtaggtgc tgattgtcga gacgctcagg ttcattctcc
     1621 cggcgatctc gccaacggtt tttcccgagc caatgaagca caacacctgg tattcccgat
     1681 ctgaaagcca ttggtgaggc tcctttgcgg cgccgggcct gatggtgacg atgagcgcct
     1741 ccgccagaga agggctcaca tatttgcctc cactccgcac cttcgtaatg gccttgacca
     1801 gctcctccgg cgcgctggat ttggtgagat acccatcagc cccggccttg accatccgga
     1861 tcgcatactg ctcctccgga tacatactga ggatcaacac cggcaatttg gggaactcct
     1921 gtttcacttg tttcagcgca tccaagccgt tcatgcccgg catgttgata tccagtacgg
     1981 ccacatccca cggcctccgc ctcagcagct ccagcatttc aaaggcgttc ccggcttcgc
     2041 cgatctttgc gccttgaaac ccttcgttca cgaggtcctt cacccctctg cggaagagag
     2101 gataatcatc gaccaccaga actttcaaca tggctccctc ttatgaggca ggctgtcccc
     2161 cgccgatagg caagcacagc gtgacggttg tgcccttttc gggatcaccc tcgatcgtca
     2221 gagttcctcc acattgagcg gcgcgctcct gcatcccgag cagccccaat gactggggat
     2281 ccgaggcctt cgtatcggga atccctacac cgttgtctgt caccgtcatc tgcagggcgt
     2341 ccggttgcga tgtgaccgtg atgatcacgg tcgtggcctg cgcatgtctc gccgtattgg
     2401 tgagggcttc ctggcaaatg cggaagactg ccgtggcccg ttccggttcc atcgcgatat
     2461 cgtcggcgct ggtcacacag gtacaggaaa taccggtgcg cttttgaaaa tcctggctct
     2521 gccactcaag tgccgccacc aatcccaggt cgtccaagag cgccggccgt aactgcgtca
     2581 ccaaccgctg caccgccgca atcgtgctat ccacttgtcc gaccatcgaa cgaaccccat
     2641 cgttcagctt ggcacgcagg tcggcccgaa cacgattccc gacgaccgac atcatgcgcg
     2701 agaggtcgat cttcaaacac gtcagtctga cccccagttc atcgtgcaac tcgcgggcga
     2761 tgcgagtgcg ttctttttcc tgcagcgtgg aaagtcgtcc acttaacgtg cggacctgat
     2821 cgagcgtggc gcgcaattgt cgttccgcgc gtttgcgctc cgtaatgtct tccaccgttc
     2881 cttcgtaccc gacaaccgcc ccggcctgat cgcgcagcgt ccgcgcgctt tcggaaatcc
     2941 aaataaagct gccgtccttt cggtacacca gtgactcgaa gccggtcacc tcctcctgtg
     3001 cctgcatcag gctaagaaac acatcgcgtc ggccggggtc cacatacagc tgactggcga
     3061 tgtccgtgat cgtcttgatc atgtcgtccg gcgagtcata cccatggatg cgcgcgagcg
     3121 ccggattcac ggtgacatac ttcccgtcgg cggtggtctg aaatatcccg tcaacggcgt
     3181 tttcaaaaat gctgtgatac cgctcctcgc ttttccgcaa tgccgcctcc acacgcttgc
     3241 gctcggtgat gtcgcggacg gacgcacagt ggtattcctt tccttcgtaa gacagaaaat
     3301 tgacactcac ctcgatggga atcatccggc cgttcttggc cttatggaag gtctctatca
     3361 ccatggtccc gcgctttctg gtttctgccc aaaaccccgg ccacatatcc gtctgaaagt
     3421 ccggattcag atcgtggacg gtcatggcgc acaactcgtc tttcgaatac cccagcatcc
     3481 ggctggctgc ctcgttcaca tccagcacct tagcctgtgg atcgatccag tacacggcgt
     3541 cagcggctcg atccaccgag aatttcgcca accgcaaggc ctcttccgcc tgtctgcgct
     3601 cgatcgcaat gccggccaga tgtgttgccc gttcaatcag ttgccggtca tgagcgccgg
     3661 gattgtgcgg ccggcgataa tacatcgcga tcgtgccgag caatcggccc tggctggata
     3721 caatcggcgt cgagtgacag gcgcccaagc cgtgggtggc cgcaagatgt ttgaagtcgt
     3781 tccacagagg atcaccggca atgtcgtcga cgaacaccgg cttgtgcttg aacgccgccg
     3841 tgccgcacga gcccgccttc ggtccgatga caaggccgtg gacggccgtg ttgtaggctt
     3901 cgggcaaact cggcgccgcg ccgtgcaata agcgttgtcc tttttcatcg gccagcagca
     3961 ccgagcaaag catgccgtcg gtggattgcg cttcggtctc gcgcatcatc gtgtcgagca
     4021 cttcgggcat cgccgttcca gtcgcaatca gttcgaggac tttcttctcc gccgccagga
     4081 gtgcctcggc ctgtttgcgc tcggtgatgt tggtgaagac gatggcgacg cggcagctcg
     4141 tctcgtcgcc cacgcgcgag gcgtggacgt tgaaccagcg gttcatcggc tcggcgtagt
     4201 gctcgaagcg cacgggctcc cctgtcaacg ccacgcgtcc gtagatctcg acccaaaact
     4261 cttcgaggcc agggacgagt tcgcgcgccg ttctgccgac ggcattgcgg agtccggtca
     4321 gttgctcgaa catcggattg atttcgagga ggagatagtc atgcggcttg ccctgttcgt
     4381 cgagcagcat ttcaacgacg cagaagcctt cgtcgatcga agtgaagacc gtgcggtact
     4441 tttcttccga ctcgcgcaat gcgacttcgg ccagcttccg ctcagtgata tccatcaccg
     4501 acccgatata cccaagaaat tcaccgtttg gtccgaagcg aggatttccc gcatcgatgg
     4561 cccggcgata ttcgccgtcg tgccgccgca ggcgatagtc gaggctgaac ggccggcgcg
     4621 ccttgttggc ctccagaaac atgcgtccgg aacgctcgcg atcctcggga tgaaccgcat
     4681 cgagccggcc gaatccgttc tgatccagcc cctcctgctc cgtctgtccg gtgaattcgt
     4741 accagccgcg cgaaatgaat gaggcagagc cgtccggttc ggtgacccag agcatggccg
     4801 gagcggtatc ggccgggttg tggaagcggg tttcgctctt gcgcagttct tgggcggatc
     4861 gatcttgagc ggtcgcgcct tcgccgccgc ccgactcgcc caccccgacg gtggaaacgg
     4921 gggttgagga gggctttgag cgttgcgcac ggagcttcgg aacggctgac ggcgagcctc
     4981 gtcggcgttt cttgtctttc atttcccgct ttcctccgga tgctcagcta tgagcgtgga
     5041 ggttcggact cagctcaatc tttttctctc aatgctttcc tctcattctg agcgtcaaca
     5101 agcttcacag cgcgcgaggt gcaaaaggcc caccccatca cctccattgg cctgagaatg
     5161 ccatgcggcg agaagaaaat atagggggtg ctaccagaac attgcgagaa ctcctttaaa
     5221 taacctgacg cgcgcagcat gagggttgtc cggaactttt gggtccactg acctcggtgc
     5281 ttcgaccaag gaggtgaagg gtaagcccga aatgcatata cgattggcct gaatgttcac
     5341 gaagtgtaga atgccgtcgg tcccttgtcc gacagcaagg aatgagaaaa gtgcttatga
     5401 gaagcgtgac atgtggcaga ataagcga
//
LOCUS       DATMTI010000294         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_393151, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000294 DATMTI010000000
VERSION     DATMTI010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393151"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2675)
                     /locus_tag="VN039_04940"
     CDS             complement(<1..2675)
                     /locus_tag="VN039_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV45367.1"
                     /translation="MHLKTLNMLGFKSFAEAKIEFPEGVTAIVGPNGSGKSNVVDAIL
                     WVLGEQSTKTLRSEKMEDVIFNGTELRKPLGMAEVSLVIGGLNPGMMKLDGSSGLPSE
                     LTEAQEMMITRRLYRNGESEYLINKIQCRLKDIRSVLLDTRAGSKGHTVIAQGQIDQI
                     LNASPQDRRELIEETAGIVRYKKQKAEALRKLEATQQNLLRVRDIVAEVKKQLNSLER
                     QARQARTFQTLQSEARGIEVTLLIREFRILRQALQEAETEVLNLDQQESEKAAEQARL
                     TTELEQARLDAIATADSIGEIREELAGVEQQQAHALTAAEVERNRGLLFSQQQVQESA
                     ELEELVRSQGNLAVALQAIESSLTSVEEELAIRDGALGELDEEMTRLLHQRAAAVEEE
                     ERGRKDVLQLAVLVANTEQGISQLAKRMEDLSGRGTRLSSERDELRSQRESAIDRHEI
                     LRKEYGEADRLVSQLRTEQRTVQEEAAQAISALQSLDQVILRRSEELAGVDSRLEALQ
                     SVVREEMGYGRQGIQQGPALKSCDGVRDAVAEWLVIPSGMERAVEAVLGERVRGWFVD
                     EPSVGRRLIRFLQQEALGRGSFIPQCPRWEGGRAYDWWPSITTQPGVIGLAVDLIQTD
                     AARTAARDSLFDRVVIVRSLDQAMQLWERHSWSAPNGPILVTLDGEVVDASGIVTGGQ
                     VQGTPGLLERRREVIDLEAKRHSLVTELDQSKQQRDTVFAQIQSLTQRDRQLGDALRA
                     AEMQNLSLRKDEEKLQHVLNDLDHRLTGMEREIQEGIGECRRLEQESHSVQAQLGQWL
                     AEKNGQETMLGRVRERLGLLDQNLRTHQERVTEAKLTAEGLRAKREHEQLNRARVTQQ
                     IQETEDRRRALGEHLNGLKGFIEQSQTEQARQEAL"
     gene            complement(2866..3828)
                     /gene="ispH"
                     /locus_tag="VN039_04945"
                     /gene_synonym="lytB"
     CDS             complement(2866..3828)
                     /gene="ispH"
                     /locus_tag="VN039_04945"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWV45368.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRQKGAVFVEELNEVPEGSVVIFSAHGVAKSVWEEANQRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYDLILIGHAGHPEVIGTLGQIPDKFHLVSSVKDVEELRVENTVNLSY
                     VTQTTLSVDECRDIVGALHQRFPNIKGPHQEDICYATQNRQNAVKELSRLVDVILVIG
                     SPNSSNSNRLRELGEQCGIPSYLIDSAADIDPEWLQGAKAVGIAAGASAPEILVTEVV
                     AFLRASEPSDVEELTVIEEDVEFLLPKELVQIESTKKSVVGIAG"
     gene            4087..>4311
                     /locus_tag="VN039_04950"
     CDS             4087..>4311
                     /locus_tag="VN039_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45369.1"
                     /translation="MAQRPFQIGAVVNLSDGESTKETVIVTVPRKKRFHTQFLGINGF
                     GHPNQPLFYAVHVTTRSRVGIYPIAPAGISE"
BASE COUNT          878 a         1363 c         1042 g         1028 t
ORIGIN      
        1 cagagagcct cctgacgggc ttgttctgtc tggctctgct caatgaaccc cttcagaccg
       61 ttcaagtgtt cacctaatgc gcgacgccgg tcttcagtct cctgaatctg ctgggtcacc
      121 cgagcgcgat tcagttgctc atgttcccgt ttggctcgca acccttctgc ggtcagcttc
      181 gcctccgtca cacgttcttg atgcgttcgc aggttttgat cgagcaatcc caatcgctca
      241 cgcacccttc ccaacatcgt ttcctgtccg ttcttctcgg caagccactg gcccaattgg
      301 gcctggaccg agtgcgactc ctgttccaac cgacgacatt cgccgatacc ttcctgaatt
      361 tccctctcca tccccgtgag tcgatgatca agatcgttca gcacatgctg aagtttttct
      421 tcatccttgc gcaacgacag attctgcatc tccgcggcac gaagcgcatc tccgagttgg
      481 cggtcccgtt gggtgagcga ctggatttgg gcaaacaccg tgtctcgctg ttgcttgctt
      541 tgatcaagct ccgtgaccag ggagtgtcgt ttagcttcaa gatcgatcac ctctcgtcgg
      601 cgttcgagca aacccggcgt gccttgaacc tgaccacccg tcacgatgcc ggacgcatcc
      661 accacttcac catccagagt taccaggatg ggaccattcg gagcgctcca cgaatgtcgt
      721 tcccacaact gcatcgcctg gtccaatgat cgaacaatga cgacgcgatc gaacaacgaa
      781 tcgcgagccg ccgttcgggc ggcgtccgtt tggatcagat ccaccgcgag tccgatgaca
      841 cccggctgcg ttgtgatcga cggccaccag tcgtacgctc gtccgccttc ccatcgtggg
      901 cactgcggaa taaaactgcc tcgccccaac gcctcctgtt ggagaaaacg aatcaaccgc
      961 cgcccgacgg acggttcatc cacaaaccac cctcgaacgc gttcccccaa cactgcttca
     1021 accgcccgtt ccatccccga cgggattacc aaccattcag ctaccgcatc ccgtactccg
     1081 tcacacgact tcaaggctgg cccctgctgg atcccctgcc gaccgtaacc catttcctca
     1141 cgaaccacgc tctgtaacgc ctcaagacgc gaatccactc cggccagctc ttccgaacgt
     1201 cgtaagatca cctgatccaa cgactggagg gcgctgatcg cttgagcggc ctcctcttgc
     1261 acggttcgtt gttccgtccg tagctgcgag acaaggcgat ccgcctctcc atattccttg
     1321 cgcaaaatct cgtggcgatc gatcgccgat tcacgttggc tccgcaattc gtcccgctcc
     1381 gacgacaagc gagttccgcg acccgagaga tcttccattc gcttggccaa ttgcgagatg
     1441 ccttgttcgg tattcgcgac aagaacagcc agttgcagga cgtcttttcg ccctcgctcc
     1501 tcctcctcga cggccgcagc ccgttggtgg agcaagcgcg tcatctcctc atcgagttcc
     1561 ccaagagccc catcccgaat cgccagctcc tcctcgaccg acgtcaatga cgactcgatg
     1621 gcttgaagtg cgacggccaa attcccctgt gatcggacca gttcttccag ctcagcggat
     1681 tcctgcacct gttgttggct gaataacagc cctcggttac gttcgacctc cgctgccgtc
     1741 agcgcgtggg cctgctgttg ttcgacaccc gccaattcct ctcgaatttc cccgatggaa
     1801 tcggcggtcg caatggcatc aaggcgagcc tgttcgagtt cggtcgtgag ccgagcctgt
     1861 tccgccgcct tctcggattc ttgttgatcc aggttcagaa cttcggtctc cgcctcctgc
     1921 aacgcctgtc gcaatattct gaattccctg atcaacaatg tcacttcaat gccgcgcgcc
     1981 tcgctctgca acgtctgaaa ggtgcgcgct tggcgcgctt ggcgttccaa agaattgagc
     2041 tgcttcttga cctccgccac gatgtctcgg acgcgcagga gattctgctg cgttgcttcg
     2101 agcttccgca gcgcctcggc cttttgtttt ttataacgga cgatgcccgc ggtctcctcg
     2161 atgagctcgc gccggtcctg cggcgacgca ttcaaaatct gatcgatctg gccctgggca
     2221 atgacggtgt gccctttgct tcccgcccgc gtgtcgagca acacactgcg gatatccttc
     2281 agccggcact gaatcttatt gatgagatac tcgctctcgc cgttgcggta caaacggcgg
     2341 gtaatcatca tctcttgcgc ttcagtcaac tcgcttggaa gtcccgaact cccatccagc
     2401 ttcatcatcc ccggattaag cccgccgatc accaacgaca cctccgccat ccctaaaggc
     2461 tttcggagtt cggtgccgtt aaaaatgaca tcttccatct tttcgcttct aagcgtcttg
     2521 gtgctttgct cgcccaacac ccacagaatg gcgtcgacga cgttgctctt tccactccca
     2581 tttgggccga cgatcgcggt aaccccctcg ggaaactcga ttttggcttc agcgaacgac
     2641 ttgaaaccca acatgttcaa cgttttcaaa tgcattggat caccaaaaat tacgcacacg
     2701 cacaacgtta tttcagagaa ttacgatcga tggggtcctt gtaccagaag tgttctgaga
     2761 aagcaaaata aaccacattg tgtggtggaa tagcttatat ggccctacca tatatagggc
     2821 cattatacag ggtcatttgg ggagatatgc ggatggcaat gtcagctagc cggcaatgcc
     2881 gactactgac ttcttggtcg attctatttg aacgagttcc ttcggcagga ggaattcgac
     2941 atcttcctcg atcaccgtga gttcttccac atcggacggt tccgaggctc tcaagaacgc
     3001 gactacttcc gtgaccagga tttccggtgc cgacgcaccg gccgcaatcc cgaccgcctt
     3061 ggctccttgc agccactcgg gatcgatgtc ggcggctgaa tcgatgagat aagatggaat
     3121 cccgcactgc tctcccagtt cgcgtagacg attggaattg gagctgttcg gcgacccgat
     3181 gaccaggatg acatcgacca agcgagacaa ttccttgacg gcattctgcc ggttttgcgt
     3241 cgcataacag atgtcttcct gatgcggccc cttgatgttc ggaaaccgct gatgcagtgc
     3301 gccgacaatg tcccgacatt catccacact caacgtcgtc tgtgtcacat acgaaaggtt
     3361 gacggtgttc tccactctca gttcttcgac atctttgacc gaggacacca agtgaaactt
     3421 gtcgggaatc tgacccagcg tcccgatcac ttccgggtgg ccggcgtgac cgatgaggat
     3481 caggtcgtat ccctgtgtat aatcgcgatt tacttcgtta tggactttga tcaccagcgg
     3541 acaagtcgcg tcgatcacat ggagacgacg ctgattcgcc tcttcccaga ccgacttcgc
     3601 cacgccgtgc gcactgaaga tgacgaccga cccttccggg acttcgttca attcttctac
     3661 gaagactgcg cccttttgcc ggagcgaatt cacgacatgg cggctatgga cgatctcatg
     3721 acgaacataa atgggggccc catatttctt gagcgacaga tccacgatat cgatcgcccg
     3781 atcgacaccg gcgcaaaatc cacggggatt ggcgagatag atcttcatag tcgtcgtagc
     3841 tccttcggcc aggctctaca aggttgtgct gtcaacgtgg ttgttcacga tacgtcagat
     3901 taccggggtc cgtcaacaga attgcctgac agacatcgct gcagcctcag tccgttggcc
     3961 ctgccaatac gcttgcttga ctctctctcc tttcctccgt aggctcgctg ggtaatgaga
     4021 ctttgcatct ttagcggcaa ctgaaacact tcccctctct attgatgatc aaaggagaca
     4081 cctcatatgg ctcaacgacc ttttcaaatc ggagcggttg tcaatctgag tgacggagag
     4141 agcaccaagg aaaccgtcat cgtgaccgtt ccgagaaaga aacgattcca tacccaattt
     4201 ctcggcatca atggtttcgg gcacccgaat caaccactct tctacgctgt ccacgtgacg
     4261 acacgttcga gagtcggcat ctatcccatt gcgcccgctg gcatttctga g
//
LOCUS       DATMTI010000295         5523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_732247, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000295 DATMTI010000000
VERSION     DATMTI010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732247"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..405)
                     /locus_tag="VN039_04955"
     CDS             complement(217..405)
                     /locus_tag="VN039_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45370.1"
                     /translation="MKRLLSRTGLLTGTLLVQALPALANAPEGGGPPDYSGITGLYYT
                     LIGVVLAYGVYDTFFKKS"
     gene            complement(506..1408)
                     /locus_tag="VN039_04960"
     CDS             complement(506..1408)
                     /locus_tag="VN039_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45371.1"
                     /translation="MQKKIPCSAGRLGLLTSQMIQAISDASAFQIAHQYLTANRVRIA
                     EADDSQITSTVIGNSGLYEQNIRLKDGHLVSKCSCTLPEEPMCRHCLAVLLEYQRWAQ
                     PRQARKPSLAKESITASPVGPSVNGKTSTVQSCSADVTLNEIMVFLEWLQPATKALER
                     QEPLPGPPALSPGPILTWIQAIRNLEDRRRESEEVMTSLESQLKDRESEVGHLTQQLQ
                     TSLRESNAAQATSQELQRKVTSYKEVLTRVSELTAEVVQHAGQMRAVTGDLLQNGSQL
                     DKLVSSFKDVAEALQSAVALPPYE"
     gene            complement(1548..2558)
                     /locus_tag="VN039_04965"
     CDS             complement(1548..2558)
                     /locus_tag="VN039_04965"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HWV45372.1"
                     /translation="MRARFLCIDSLGRRSAVMLGLFVSLLAFPIGCAAERDANLRHVL
                     DKGWQPWQILETQTGRVIPFSEFMTNLERYDIVYLGEEHHNPYHIQAALKVLDQLVAD
                     GIEPTIGMEMFGWDGQSALDDYVATPQPVTNEFLEQVRWKQNWGGAFEDYAPLVTFAR
                     DRHLSVRAMNPPKPLIRRVVKLGLDQAKQEPEWEPWGILQEDIVDDPAYRERIVDQLR
                     RCHGGSEEHFRTMYEASMVRDEGMARTLAIRQKEFRRENGGPRRLIVSYTGGGHVQYN
                     LPVPKRVAKRLGGDIEQTTIYMTSFEPGKTADVQALIQEPIADYIWLTPMGKSGSSKP
                     CR"
     gene            2601..3995
                     /locus_tag="VN039_04970"
     CDS             2601..3995
                     /locus_tag="VN039_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45373.1"
                     /translation="MTPKFEDYYAPYPTVLDTLAHTADQPPMSPDPLAEFRRVVSFRA
                     RQFPRQWEASKKLMEGATFPSTIARLCEAIQNKDLPASVKEILLRLFERDTPRRVLDL
                     DGDSLKSVTGLPPAKALRALAVFFGLVPAPVARWPVTHLSSDEVEEVLRHLKNPFDLL
                     CRSDVASVLDIGAGDLSFVEELVDLYGSEPERQQRQFIVHCVDRLDPRSQLGGPLHAN
                     TERLQKLQRKEGLSFSFFGNQDMFELGDLDKQEMLAPRYTIATCWAPATPTFAYEPSR
                     LSETLIRSELERTKGTFSQTRFGKEHALEVRHAGRALLFPPWKFEIAGPLALLNLLAR
                     RGFLCVLGSVDAQVFWELLAQLLEDPRYRPPDQLFDSVTLPKVFGEVYHILAGLPIGE
                     SIDLAEVALLRRHYLHLPPDSSAFMDSVGGHFRYIRISRGATFPDTPASSTARKFASM
                     TEEVPPWLLTLVPA"
     gene            4145..5086
                     /locus_tag="VN039_04975"
     CDS             4145..5086
                     /locus_tag="VN039_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWV45374.1"
                     /translation="MNPTDIIRAIQENIARVIKGKPHVIEMSLVALLARGHLLLEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGVSIFNRQKQAFEFIPGPIFANIV
                     LADEINRTTPKTQSSLLEAMSEAQISFDNKTYPLNQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPSPEEEKRVLDRPQSLHPAEELQPLLTAHDVLLLQEQVDHVLMEE
                     SITDYLLSIVQSTRQSDLLSLGVSTRGALALSRAAKALSLVRGRTYCLPDDIKELAPI
                     VLSHRIMVARAQGLRQRSFEQAERIIQDLVDSIPVPV"
     gene            5129..>5523
                     /locus_tag="VN039_04980"
     CDS             5129..>5523
                     /locus_tag="VN039_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45375.1"
                     /translation="MRRQSPSFIRRLFRHRSTSVTSEGVQFLFFTLAIGIAAVNTGNN
                     LFYLLLAMMLSLILTSGIVAEYCLRRLEFHRHLPDHLIVNEPTVAMLVVANRKSRLPS
                     FSLKLFDMSDGRELDRGLEIPQLLPGGSR"
BASE COUNT         1182 a         1578 c         1467 g         1296 t
ORIGIN      
        1 ggttcatcct cgttgaatca agctgacggc attcatccga cgaaggaagg ttatgagatt
       61 atcgtagacc aagtactcaa agttctcaaa ccggtcttga atgatcggct ccacccttcg
      121 actgctgaca agcagagttg aaggcgagac ctctcgacca gaacccgcac gatgtaagac
      181 aaaccaatag aagacgggtc agcgaagcgc taccccttac gactttttga aaaacgtgtc
      241 gtaaacgccg taggcgagga ccactccgat caacgtatag tataggcctg tgatcccgct
      301 gtaatccgga ggccctcccc cctctggggc gttcgccaac gccggtaaag cttggaccag
      361 caaggttccc gttaacaaac ctgttcgtga aagtagccgt ttcatctctg cccccttcca
      421 aatccgcaca cgaaaagtgg gtgcattcta ctggacgtcg gttggtaggt gcaagaggag
      481 aatcggcata gggacgggtt agtcgttatt cgtagggagg cagcgcgacg gctgactgga
      541 gtgcctcagc cacatcctta aacgaactga ctagcttatc gagctgagag ccgttctgga
      601 gtagatcgcc cgtaacagcc cgcatctgcc cggcatgctg cacgacttcg gcagtcaact
      661 cgctgactct ggtcagcact tccttgtagg aggtcacttt gcgttgaagt tcctggcttg
      721 tggcctgcgc ggcattgctc tctcgcagag aggtttggag ttgttgcgtg aggtgtccaa
      781 cctccgattc acggtccttc aattgagatt cgaggctcgt catgacctct tcactttccc
      841 tgcgtcgatc ctcaaggttc ctaatcgctt gaatccacgt caagatcggt ccaggactga
      901 gtgcgggagg accgggaagc ggctcttgtc tttcaagagc tttcgtcgcg ggttggagcc
      961 attccaaaaa gaccatgatc tcgttaaggg tgacatcggc agagcacgat tgtacggtgg
     1021 acgtcttccc gttaaccgaa ggaccgaccg gagatgccgt gattgattct ttcgcaaggc
     1081 ttggctttcg tgcttgccga ggctgggccc atcgctgata ttccagcagc accgcgaggc
     1141 aatggcgaca catgggctct tcgggaagcg tacaggaaca tttcgagacg aggtggccgt
     1201 ccttcagtcg gatgttctgc tcgtacagac cggaattgcc gatgacggta gacgtgatct
     1261 gggaatcatc ggcctcggcg atacggacgc gattcgcggt caagtattga tgggcgattt
     1321 gaaaggccga ggcatccgaa atggcctgga tcatttgaga ggtcaacagt ccaagccgcc
     1381 cggcgctgca aggaattttc ttttgcatct aaatgcgcgg atctcttgca caaccactaa
     1441 gcatgatggc ctcagtgtga cgagcgcctt gaatcctgtc aagcactcaa cggttttgtg
     1501 ttgccctcgc ttgagcgact gtctgaagaa taaggttctg agacggttta ccggcagggt
     1561 ttagacgaac cggacttacc catgggtgtc agccaaatat agtccgcgat tggttcctga
     1621 atgagagctt ggacatcggc tgttttaccg ggctcaaacg acgtcatgta aattgtggtc
     1681 tgttcgatgt cccctcccag gcgtttggcg acccgcttcg gcacggggag gttatattga
     1741 acatggcccc ctcccgtgta actcacgatc agacgacggg ggccaccgtt ctctcggcga
     1801 aattcttttt gcctgatggc cagcgttctc gccataccct catcccggac catcgatgcc
     1861 tcatacatcg tccggaagtg ctcctcgctt cctccgtggc accgtctcaa ctgatcgaca
     1921 atcctctcgc ggtacgctgg atcatcgacg atatcctcct gtaggattcc ccacggctcc
     1981 cattcaggct cttgcttggc ctggtcaagt cccagtttaa cgacgcgacg aatcaagggt
     2041 ttgggtgggt tcatcgcccg gacggacaag tgccgatctc gagcgaacgt caccaatggc
     2101 gcgtagtctt caaacgcgcc accccaattt tgtttccagc gcacctgttc caaaaactcg
     2161 ttcgtgaccg gttgaggtgt ggcgacgtag tcatcgagtg ccgattggcc gtcccagccg
     2221 aacatctcca ttccaatggt gggttcaatg ccatccgcta ccaattgatc cagcactttc
     2281 aaggcggctt ggatatgata tggattgtga tgttcctctc ccagatagac aatgtcatac
     2341 cgttccaggt tcgtcatgaa ttctgaaaat ggaatcacac gcccggtttg agtttcaaga
     2401 atctgccagg gttgccatcc cttatcgagg acgtgacgca gattggcatc acgttcagcc
     2461 gcacagccga tcgggaacgc cagcaggctg acgaacaggc ccaacatcac ggccgatcgc
     2521 cgacccagag aatcgatgca caggaatcgc gctctcatga aaccactcct accacacaga
     2581 atgagcatga acaagccgac ttgacgccga agtttgaaga ttactatgct ccttatccaa
     2641 cagtccttga taccctcgca cacacagccg atcagcctcc catgagtccc gacccactcg
     2701 ctgaattccg tcgcgtcgtc agttttcgtg cgcgccagtt tccccggcaa tgggaggcat
     2761 caaaaaagtt gatggaaggt gccacctttc cgtccacgat cgcgcgactg tgcgaggcca
     2821 ttcagaacaa agaccttcca gcttcagtga aagaaatctt gttgcgcctc ttcgaacggg
     2881 acacgccacg gcgcgttctg gatctcgatg gagacagcct caagtccgtt acgggccttc
     2941 ctcctgccaa agccctgcgt gcgcttgccg tcttttttgg gttggtgccc gcacccgtcg
     3001 cgaggtggcc tgtgactcat ctgtcgagtg atgaagtcga ggaagtcctg cgccatttga
     3061 aaaatccttt cgacctcctt tgtcgcagtg acgtggcatc tgtgttggac atcggggccg
     3121 gcgatctctc gttcgtcgag gagttagtcg atctgtacgg ctctgagccc gagcgacaac
     3181 agagacagtt cattgtccac tgcgtggacc gactcgatcc ccgttctcaa ctgggaggtc
     3241 cgttgcatgc gaatacggaa agactgcaga agttgcagcg gaaggagggg ctatccttct
     3301 cgttcttcgg caatcaagac atgtttgaac tgggagatct ggacaaacag gagatgctgg
     3361 ctccacgata taccatcgcg acgtgttggg caccggccac accgaccttc gcctacgaac
     3421 cctccaggct ctctgagacg ctcattcgca gcgagcttga gcgtaccaaa ggcacattta
     3481 gccagacacg ttttggcaaa gagcacgcgc tcgaagtccg gcatgccggc cgcgccctcc
     3541 tcttcccacc gtggaagttt gagatcgccg gaccccttgc cctcttgaac ctgctcgccc
     3601 gtcggggatt cctgtgtgtg ctgggatcag tggatgcaca ggtgttttgg gaactcctgg
     3661 cgcagttgct ggaggacccg cgctatcggc caccggacca actctttgac tccgtcaccc
     3721 tccccaaggt cttcggcgag gtctatcata ttcttgccgg tctgccgatc ggcgaatcaa
     3781 ttgatttggc tgaggtggca ctccttcggc gtcattatct tcatcttccg ccggactcat
     3841 ccgccttcat ggatagcgtt ggcggtcatt ttcggtatat ccgcatcagc agaggtgcca
     3901 ccttcccgga cactccggcg agcagtaccg cacggaagtt tgcttccatg accgaagaag
     3961 ttcccccatg gcttctcaca ctggttcccg cataagctga tccccagctc cgtggcttaa
     4021 tgccgggtcg tagagagccg caaagcgctt gaataattga ccgtttccag accctttgtt
     4081 agacttcaac cgtgtcggat caccagggca ttctgcagcg gcatcacaca ccgtagtcct
     4141 catcatgaat ccaacggaca tcattcgagc catccaggag aatattgcgc gcgtgatcaa
     4201 gggtaaaccg catgtgatcg aaatgagcct ggtcgcactc ttggcgcgcg gacatcttct
     4261 cctcgaagac gtaccgggag tcggaaaaac gacgctcgcg catagcctcg cgcgatcgct
     4321 cgattgctcc ttcaagcgca ttcagttcac gagcgatctg ctgccgtccg atatcgtggg
     4381 agtctcgata ttcaatcgac agaaacaggc ttttgaattc ataccgggtc cgattttcgc
     4441 caacatcgtg ctcgcggatg aaatcaatcg gacgacaccg aagacacaaa gtagtctatt
     4501 ggaagcgatg agcgaagcac agatttcgtt cgacaataag acctatcctc taaatcagcc
     4561 attcatggtc attgcgacgc agaatccagc cgaatatcac ggcacgtttc ccctcccgga
     4621 gtcgcagctg gatcgattct tgatgcggct tcgcatcggc tatccctcgc cggaagaaga
     4681 aaaaagggtt ctcgaccgac cgcaatcgct ccacccagcc gaagaactcc aaccgttgct
     4741 gacggctcat gatgtccttc tgctccaaga acaagttgac catgttctca tggaggaaag
     4801 tattacggac tatctcttgt ctattgttca aagcaccagg cagtcggacc tactgtcatt
     4861 gggagtgagt accagaggag cgttggcgtt gagtcgggct gccaaagcgt tgtcgctcgt
     4921 ccgcggccgg acctattgtc tgccggacga tattaaggaa ttagccccca tcgtgctctc
     4981 tcaccgcatc atggtggccc gcgcgcaagg gctgcgtcag cgaagctttg agcaagccga
     5041 gcggatcatc caggatttgg tcgactcgat tcccgtccca gtctaggtca tccctgaagt
     5101 ctgtgtcccg gcgcggagct ggtcgtacat gcggcgtcaa tctccttcct tcattcggcg
     5161 attgtttcgc catcggtcga cgagcgtgac ctcagagggc gttcaattcc tgttctttac
     5221 attggcaatc ggcatcgcag ccgtcaatac cggcaacaat ctcttctatc ttttgctcgc
     5281 gatgatgttg agcctgattt tgacttcggg aattgtggcg gaatactgcc tgcgacggtt
     5341 ggaattccat cggcatcttc ccgatcatct cattgtgaat gaaccgaccg tcgccatgct
     5401 ggtcgtggcg aatcggaagt cacggctccc gagcttttcc ttgaagctgt tcgatatgag
     5461 cgacggccgt gagctggatc gaggtttaga aattccccaa ctccttccgg gtggcagccg
     5521 gat
//
LOCUS       DATMTI010000296         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_435310, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000296 DATMTI010000000
VERSION     DATMTI010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435310"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..>2249)
                     /locus_tag="VN039_04985"
     CDS             complement(88..>2249)
                     /locus_tag="VN039_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV45376.1"
                     /translation="RYVRKDGSIVWVLLNVAPLRKLGEELSQHIAVVQDITERKHAEE
                     RLRENEERFRMLAEAMPHFVWHTDEQGETEFENQRWYDYTGLTHETTRHGGWFTVQHP
                     DDAPRLADAWKKAVETGGEYDIETRFRRDADGAYRWFRVRGAPVRDAEGRIRSWVGTC
                     TDIHDRKEAELALRESEERYRATFANAAVGIAHIGLDGRWLQFNDSLCTITGYFREEL
                     LTMTFVDITSPGDLKTEWAQARRMLAGEIETYSMEKRYVRKDGSLIWVNKTVSLLRDA
                     CHRPLHFICIIEDIDDRKQAEEALREAQARLYRWNVELEQAVNMKTAELRQSQDHLRA
                     LTSELNLAEQRERKRLATELHDHLQQMLVVGKLTIGQGKRAALGVPACETVLKKVDDI
                     LSDALTYSRTLVAELSPPVLRDHGLTASLKWLAEYMKKKHEQTVAVVVLDDQGLNLPE
                     DQRVLLFQSVRELLINSAKHAGTGQAALTMEKQADHLCITVKDEGKGFDLAAAAAAGV
                     PSGGISSKFGLYSIQERMRALGGSFTIDSAPGHGTIATLILPLVSSADDNQPHPSVTE
                     SEDAWATVPADEADSLTKGRMMVSVLLVDDHAMVRQGLRSVLDAYADIQIIGEARDGV
                     EAVKSVEKLRPQVVVMDINMPKMNGIEATMEIKTKWPETTIIGISVNVGDDNNDAMQR
                     AGAVTLLTKEAAVEQLHDTIVQAVGSQDTQGGSVIPR"
BASE COUNT          434 a          725 c          566 g          524 t
ORIGIN      
        1 accgggcact gacctctatt cgttcagccg ccactctggt cgacgcggga caccaagata
       61 gcatccctaa aaagagtctg agaagatcta tcgcggaatc accgaacctc cttgcgtgtc
      121 ttgtgaaccg acggcctgaa cgatcgtatc gtggagttgc tccaccgctg cttctttggt
      181 cagcagtgtg accgctccgg ctcgctgcat cgcatcgttg ttgtcgtccc cgacattcac
      241 ggaaatgccg atgatcgtgg tctccggcca cttggttttg atctccatcg tagcttcaat
      301 cccgttcatc ttcggcatat tgatgtccat caccaccact tgaggtcgga gcttctcgac
      361 cgatttcacc gcctccacgc cgtctcgggc ctctccgatg atctgaatat ccgcataggc
      421 atccagcacc gatcgcagcc cttgccgcac catcgcatga tcatccacga gcaacaccga
      481 caccatcatc cggcctttcg tgaggctatc ggcctcatcg gcaggaactg ttgcccaggc
      541 atcctcggac tcagtgactg aaggatgcgg ctggttgtcg tccgcgctgc tgaccagcgg
      601 caagatcagt gtcgcaatgg ttccgtgccc aggagcggag tcaatggtga acgagccgcc
      661 caatgcccgc atccgctcct ggatgctata taaaccaaac ttggacgaaa tcccgccgct
      721 cggcaccccc gccgctgctg cagcggcaag atcgaacccc ttcccctcat ccttcacggt
      781 gatgcaaaga tggtccgctt gcttttccat tgtgagagca gcctgccccg ttccggcatg
      841 tttggccgaa ttgatcagaa gttcccgcac cgattgaaag agcaacacac gttgatcttc
      901 cggcaggttg agaccttgat cgtcgagcac aacgacggcc acggtttgct catgcttttt
      961 cttcatgtac tcggcgagcc atttaagact ggcggtcagg ccatgatcac gcaggaccgg
     1021 cgggctgagt tccgccacca atgtccggct ataggtcaac gcatcggaca ggatatcgtc
     1081 gaccttcttc aagacggttt cacaggccgg cacgccgagt gccgcccgct tgccctgccc
     1141 gatcgtcaac ttgccgacga ccaacatctg ctgcagatgg tcatgcagtt cggttgccag
     1201 gcgtttccgt tcacgctgtt ccgccaggtt caactcgctc gtcaacgcgc gcagatggtc
     1261 ctgcgactgc cgcaattccg ccgtcttcat gttcaccgcc tgttcgagct ccacgttcca
     1321 acggtacaag cgcgcttgcg cctcgcgcag cgcttcttcc gcctgtttcc gatcatcaat
     1381 atcctcaatg atgcaaatga aatgaagcgg cctatgacac gcatctcgaa ggagcgatac
     1441 cgtcttattc acccagatga gcgaaccatc ctttcgtacg tagcgctttt ccattgaata
     1501 ggtttcaatt tcacccgcca gcatgcgacg tgcttgcgcc cactctgttt ttagatcgcc
     1561 gggagaggtg atatccacga acgtcatagt gagcaattct tcacggaaat agccggtgat
     1621 tgtgcataag gaatcgttga actgtaacca ccgaccgtcc agtccgatgt gagcgatgcc
     1681 gacagccgca ttggcaaagg tggcgcgata gcgctcttcg ctctcgcgta gagccagctc
     1741 cgcttctttg cggtcatgga tatcggtgca ggtccccacc catgaccgaa tgcgcccttc
     1801 agcatcccga accggcgccc ctctcacacg aaaccatcga tatgctccgt cggcgtcgcg
     1861 gcgaaatctt gtttcaatgt cgtactcgcc gcctgtctcg acggctttct tccacgcgtc
     1921 agccaatctt ggcgcatcgt cggggtgttg gaccgtgaac cagccgccgt gtctcgtcgt
     1981 ctcatgcgtc agtcccgtat agtcgtacca ccgttgattt tcgaactccg tctcgccctg
     2041 ctcgtcggta tgccacacga agtgcggcat cgcttcggcc agcatacgga acctttcttc
     2101 gttctcgcgt aacctttctt ccgcatgctt gcgctccgtg atgtcctgga caacggcgat
     2161 gtgctgggag agttcctctc ccagcttccg gagcggagcc acattgagca ggacccagac
     2221 aatcgaccca tccttccgca cgtagcgtt
//
LOCUS       DATMTI010000297         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_732318, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000297 DATMTI010000000
VERSION     DATMTI010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732318"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VN039_04990"
     CDS             complement(<1..914)
                     /locus_tag="VN039_04990"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HWV45377.1"
                     /translation="MFYSVPFRIGKILLLLASYVFYMWWDPRFIVLILTSTIVDYFLG
                     IWLETASGRRKKLLLVVSLVVNLGILGFFKYYDFFAGSLAALLRIPQSSVVLQIVLPV
                     GVSFYTFASLSYTFDVYWGKMKAVRNPLDYALFIAFFPHLIAGPIIRARQFIHQIWSW
                     RSPTAIVVQSGIILVLSGLIKKMVFADRFAVVSDSYFNDPTAHPGWLAAWSGCLAITM
                     QVFFDFSGYTDIARGCAKLLGFEFPLNFARPFLARNPPDFWQRWHITLSTWVRDYMFM
                     PFSRGRKGRLVLYRNMFVVMVLIGLWHGA"
     gene            complement(988..2502)
                     /locus_tag="VN039_04995"
     CDS             complement(988..2502)
                     /locus_tag="VN039_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45378.1"
                     /translation="MLDDGTGKTEITWDTGNGAVGFVFVTEENRKPVAFASGSRGKQA
                     APWIKEHSYLFELYGDDQRQTLLAKVAVSGSAGNMSAQGTSRWQSIAHWALIVGLAAI
                     VYVAVYFSSTGPVRTTFPTEPTTSPRPLHVRRNLFLGIVAFICVDGAIFHSGLYVSIL
                     APSSYAGRIARITRAERERASSGLKEVLVLGDSRIAEGFSAKLANELGSAAGLKFVSL
                     AEPAASANTWYYMVREVDPTARRYAAIIVPYGIGYDPNSADQLRISMTAPLLRYADSF
                     HFASGFQRWSGRVRAFVASILRGSAYQDDVVDFLAHPIARITNVRQANQRHSGSEYKG
                     REYDLVGTSYDATTGQVTYAPKLTEAQRSAFRKSLIRPSESDTEYSLKLQRDWIPRIM
                     HRYADSPTAIVLTPVPRGPFLQLAGFSTLHESVLPTSVIEKTNFSLPGHTFDFLEKPE
                     YYFDAFHLNAKGRQKFTENLVSELIARLPVVSKPRASARVELPVPGPRSTDEMN"
BASE COUNT          718 a          748 c          728 g          588 t
ORIGIN      
        1 gctccatgcc acagaccaat gagcaccata acaacaaaca tgttgcggta taacaccaga
       61 cgccctttcc tcccccggct gaatggcata aacatgtagt cacgcaccca tgtggaaagc
      121 gttatatgcc agcgttgcca aaagtcaggc gggttccggg ccaaaaatgg gcgcgcgaaa
      181 ttaagcggaa actcgaaacc aagcagtttg gcgcaaccgc gagcaatgtc tgtgtacccg
      241 gaaaaatcga agaacacctg catggtaatg gcaaggcaac ccgaccacgc agccagccaa
      301 cccggatgcg cagtaggatc attgaaatac gaatcggaaa ccacggcgaa ccggtccgca
      361 aataccattt tctttatcaa gcctgacagg acgagaatga ttccgctctg gacgacaatt
      421 gctgttggag atcgccatga ccaaatctga tggatgaatt gccgcgcacg gataatcgga
      481 ccggcgatca ggtgaggaaa aaatgcgatg aacagcgcgt aatcgagcgg gtttctgacg
      541 gccttcatct ttccccaata gacgtcaaag gtgtaggaaa gactggcgaa cgtgtagaag
      601 ctgacgccga cgggaagcac gatttgcaac accactgacg actgcggaat gcgaagcaac
      661 gcagcgagcg agccggcaaa gaaatcgtaa tacttaaaga atccaagtat tccgagatta
      721 actacaaggc tgactaccag cagaagcttc tttcgccggc cgctcgcagt ctccagccag
      781 attccgagaa aataatccac gattgtagag gtgaggatga gcacgatgaa tcgcggatcc
      841 caccacatgt agaagacata gctcgcaagt agcagcagga tctttccgat acggaacggc
      901 acgctgtaaa acaccgccgc aacgatgaaa aagaagatcc aatactgaaa actgttaaag
      961 agcatgcaat actcgttagg ttaactccta attcatctca tccgttgagc gcggtccagg
     1021 cactggcaat tcaactctcg cgctagctcg tggtttcgaa acaaccggaa ggcgtgctat
     1081 taactccgat acaaggtttt ccgtgaactt ctggcggccc ttcgcgttca gatggaatgc
     1141 atcgaagtag tattccggtt tttcaagaaa atcgaacgtg tgtccgggta acgaaaaatt
     1201 tgtcttctcg atgaccgagg tcggaaggac cgattcatgt agtgtcgaaa atccagcgag
     1261 ctgtagaaac ggtccgcggg gaacaggcgt aagcactatc gcggttggac tatctgcata
     1321 gcgatgcata atgcgcggga tccaatcgcg ttgcagtttg agcgagtatt ccgtgtccga
     1381 ctcggacgga cgtatcaagc ttttccggaa agccgatctc tgagcttctg ttaacttcgg
     1441 cgcgtacgtt acctgtcctg tcgtcgcatc gtaagatgtc cccaccagat cgtactcccg
     1501 gcctttatat tccgatccgg aatgtctttg gttcgcctgt cgaacgtttg taattcgcgc
     1561 gatcggatgc gcaagaaaat cgacaacatc gtcctggtac gccgagcctc gcagtatgga
     1621 agcaacgaac gccctgaccc ggccagacca tcgttgaaaa ccagaggcaa aatgaaagct
     1681 gtctgcatac cgcagtagcg gagctgtcat ggaaatccga agctgatcgg cgctgtttgg
     1741 atcgtagccg atgccgtacg gaacgatgat cgccgcgtag cgccgcgcag tgggatccac
     1801 ttcccgaacc atgtaatacc acgtgttagc cgatgcggcg ggttcggcga gactaacaaa
     1861 cttcagtccc gcagcggacc cgagttcgtt cgccaatttg gcggagaacc cctcggctat
     1921 ccttgaatca ccgagcacca ggacttcttt caaacctgat gaagcgcgtt ccctttcggc
     1981 tcgggtgatt ctcgcgatac gtcccgcgta cgaacttgga gccaggatcg acacatacaa
     2041 tccgctgtga aagatcgcgc catcgacaca aatgaatgcg acgatgccaa gaaagagatt
     2101 tcgtctgacg tggagtggcc ggggcgacgt cgtcggctcg gtgggaaatg ttgttcgcac
     2161 cggcccggtg gaactgaagt acaccgccac gtacacaatc gctgccagcc caacgatcag
     2221 tgcccagtgc gcgatcgatt gccatcgcga ggtgccttga gcagacatat tcccggcaga
     2281 tccggaaaca gcgactttag cgagcaacgt ctgccgttga tcgtcaccat aaagttcgaa
     2341 gagatagcta tgctccttaa tccatggtgc agcctgttta cctcgcgaac cgctcgcaaa
     2401 cgcaaccggt tttctgtttt cttcagtgac aaaaacaaat cctaccgcgc cgtttccggt
     2461 gtcccacgtg atctcagttt ttccagttcc gtcgtcgagc atcacgcgct gcgggtttgc
     2521 tgtgatagaa ggtgcgcgcg aaactccggc tgaattgcta ccggatgaat tttgttcagg
     2581 cgcagcgtcg agaacggatg cgtttcccga atatatcgca acacacacca cgaggccggc
     2641 caggacgctg cgaatcttca gaagcactgg cctatcacgc tgtcgttcgt gaaagcactt
     2701 atgcagttcg cgtgtcatgc gagcgtcata attcggcgtc aacaaatcgg ctctggttca
     2761 tggcgctcaa cttctttgaa tg
//
LOCUS       DATMTI010000298         7036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_774820, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000298 DATMTI010000000
VERSION     DATMTI010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774820"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VN039_05000"
     CDS             <1..1155
                     /locus_tag="VN039_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV45379.1"
                     /translation="TANNKVDRKALPAPDSTLASSAVYVAPRDRMDIQMAVLWQQVFE
                     IDKVGIHDNFFDLGGHSLRAARLFHLIEQIYGRHLPLSTLFQAPTIAGLTSVLAQEQW
                     TPPWQSLVAMQPNGSAIPIFLVPGVMGNVLWLAQLARLLGQTHPVYGLQARGLDGDTA
                     PFGSVPEMAQQYIAEIRTCVPQGPYVIVGACTGGLVAYEMAQQLAEQERAVILGIINS
                     WHPSSYHQHYDTVHPSRLALPLGILLQTLRTVRELRRMSMSEWWSVIQRKCKSFLSIF
                     QGPTGNELRQRQIKRVRQAMFHAAAHYTMRPYPGHILNIVASQRIESDDTRYAWRELA
                     GGGCRTIDMSALRTADLLVSPHVEEVSSHIRRYIAECSQDTPVQPNSRAA"
     gene            1213..1977
                     /locus_tag="VN039_05005"
     CDS             1213..1977
                     /locus_tag="VN039_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HWV45380.1"
                     /translation="MLISFLSIERLADTSRQDHICLEPDSVHLWGIELDGSQRCLERC
                     SGWLDDVERHRAARLVREDTRQRYMLTHGGLRAVLSRYLGVNPDVVALDRSATGKPFL
                     KNELRDRLPITFNLSHAHDRALIAISKGQEVGVDLEFVRADIEVANLSQRFFTPSEHA
                     AIMQATEERRATRFFRYWVAKEALLKAQGIGLGGLPDCEIVFEADGVDTDVRARLGAR
                     FPNTLRVRLLPCEKGWEAAVAAQRLGAVKQCGLDSL"
     gene            complement(2026..2664)
                     /locus_tag="VN039_05010"
     CDS             complement(2026..2664)
                     /locus_tag="VN039_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HWV45381.1"
                     /translation="MMNYRLAHVACALLVLTAAGCLSPRTDSSKIHTYQLSLDGWPSE
                     AQPGKLDGPVLLVSPPQAEPGFETQRMVYVKRPHELEYYAVNQWADMPVRMFAPLVVQ
                     ALNQQNDAWRAVIPLPSSILGDYRLDTHGFLLQQEFLQQPSRVRVMVRAQLVELKGST
                     ILSTRAFEVVENATSENPYGGVQAANRAIAGLLDQIGSWLRQCVRHSPECSR"
     gene            complement(2689..3651)
                     /locus_tag="VN039_05015"
     CDS             complement(2689..3651)
                     /locus_tag="VN039_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWV45382.1"
                     /translation="MEPKVNYILVGSFVAFLGAAVLVGILWLGKTDYRGAYDRYEAYM
                     RESVAGLSVDSTVKYRGVDVGRVKTIALNPNNPEEVLLTMEIMHGTPIKTDTIAVLET
                     QGLTGLATINLTGGSRDAPSLQALEGQIYPIIKTGPSLLFRLDEAVSRLLSEEGLAQL
                     LVDLDTAAKGAAKVLDEDNRTMLKRTIKDLSDVTQTIAAHKTQIEQSLNGAARSADNL
                     VKLTAALNAEVPTLLAGINKSVAALGTATDELARTSKTVGTVVNEARPELQQFTRRTL
                     PEAGLLVTELRQLTGTLTRVAREVEREPSSLVFGRKAPSRGPGE"
     gene            complement(3780..4589)
                     /locus_tag="VN039_05020"
     CDS             complement(3780..4589)
                     /locus_tag="VN039_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV45383.1"
                     /translation="MPSATNIETPVIDVSHVATKFGQAVVHEDVSLSIRRGEIFAIAG
                     GNGCGKTTLLREIIGLITPSAGRIRLFGIDSRQLETGDSHPIHRRFGVMFQHGALFSS
                     FTLAENVAVPLREHTTLSDGLIRDIVAAKIAMVGLPPDSATKYPNELSGGMRRRAALA
                     RAIVMDPELLFLDEPTAGLDPIIAAGFDDLVLSLKDLLGLTVVMVTHDLDSLWRIANR
                     VAVLGRGKVLGIGTMQELSRSDDPVIREYFQGPRGRAASEQAVWNQERRET"
     gene            complement(4612..5718)
                     /locus_tag="VN039_05025"
     CDS             complement(4612..5718)
                     /locus_tag="VN039_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV45384.1"
                     /translation="MEQATISIKDNVILCRGSWTLPHLSQLERGGRALRWPDTPSVLY
                     DAGEVTAMDTGGALMMQRCIDGLRRNGQQTTLQGLKPEFAELLRKVEVQWSRPERGVS
                     VGTRWMDGLVRVVQSRQVSAIRALAFVGESTIAFSRALIRPHSIRWRTLLRIVDLDGV
                     RALPITGLLTFLVGVVIAYQGAEQLRKFGTNIFIVDLVGISLLREIAPLIVAILIAGR
                     SGSAYAAEIGTMKVTEELDAVRTLGISPMNLLVLPRALALIIALPLLTVYADVVGVFG
                     GMLIALGELNVSFVEFIARFEEAVPVRHFLIGLGKAPFFAAIIALVGCYQGFQIRGGV
                     DDVGRHTTISVVQGIFLVIVFDAICSILLNWWDL"
     gene            complement(5816..>7036)
                     /gene="ftsH"
                     /locus_tag="VN039_05030"
     CDS             complement(5816..>7036)
                     /gene="ftsH"
                     /locus_tag="VN039_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWV45385.1"
                     /translation="LVGPPGTGKTLLARAVAGEAGVTFFSISGSEFVEMFVGVGAARV
                     RDLFEQAKGKAPCIIFIDELDALGKARGSGPMGHEEREQTLNQLLVEMDGFDPRIGVI
                     LMAATNRPEILDPALLRAGRFDRHVVVDRPDKIGRLAILRVHAKQVALGPDADLENIA
                     AMTPGFSGADLANIINEAALLAVRRGKDQVGISELQEAVERVIAGLEKKNRVLNKMEK
                     ERVAYHETGHALIAMSLPGMDQVQKISIIPRGVAALGYTLQLPTEDRFLMTKSELENK
                     VAVLLGGRIAEETIFGEASTGAQNDLVKATDIAKSMVKAYGMSEKLGTITLERERQPQ
                     FLQLPVASEKGDYSEETAREIDCEVRRIVDEQYGRVRRLLEDRKAALRQGAQLLLERE
                     VMTGAELKAILEAA"
BASE COUNT         1486 a         2159 c         1909 g         1482 t
ORIGIN      
        1 accgcaaata acaaggtcga caggaaagca ttaccggctc cagactccac tctcgcgagt
       61 agcgctgttt atgttgcacc gcgcgatcgc atggatatac aaatggcggt cttgtggcaa
      121 caagtattcg agatcgacaa ggtcgggatt cacgacaact ttttcgatct cggcggccac
      181 tcgctgagag cggctcggct gttccatctc attgaacaga tctacggccg ccatcttccg
      241 ctttccacgc tgtttcaagc gccgacgatc gccggcctca cgtcggttct tgcgcaggag
      301 caatggacac ccccatggca atcattggtg gccatgcagc caaacgggtc ggccatccct
      361 attttcttgg ttcccggagt aatgggcaat gtgttatggc tcgctcagtt ggcgaggctc
      421 ttaggacaaa ctcacccggt gtacggctta caagcgcgag gattggatgg ggacaccgca
      481 ccattcggtt cagtgccgga gatggcgcag cagtacatcg ccgagatccg cacatgcgtc
      541 cctcagggac cctatgtgat cgtgggagca tgtaccggtg ggctggttgc ctatgagatg
      601 gcccaacaat tggcggagca agagagagcg gtcattctag gcattataaa tagctggcat
      661 ccgagttcgt atcatcagca ctatgatacc gtccacccaa gtcggcttgc attgccgttg
      721 ggaattctct tgcagacctt acgcaccgtc cgcgagctcc ggcgcatgtc catgagtgag
      781 tggtggtcgg tcatacagcg caaatgcaaa tctttcttgt ccattttcca gggtcctacc
      841 gggaatgagc tgagacagcg tcagatcaaa cgcgtccgac aggcgatgtt ccatgcggcc
      901 gcccactata cgatgcgccc ctatcccgga cacatactca acatcgtggc ttcgcaacga
      961 attgagtcgg acgataccag atatgcttgg agagagctgg caggcggagg atgccggacc
     1021 atcgatatgt cggcccttcg aacggcagac ttgcttgttt caccgcacgt cgaagaagta
     1081 tcctcccaca ttcggcgcta tatcgccgaa tgttctcaag atacacccgt tcagccgaat
     1141 agtagagctg cgtgattttc accaccttcc tttgccgaac gacgaaggcg attcggcatt
     1201 cacggtaaca atgtgctgat ttccttcctg tccattgagc gcctcgccga tacaagccga
     1261 caggatcata tctgtcttga accggactcc gttcacctgt gggggatcga gcttgacgga
     1321 tcgcaacgct gtctggagcg atgcagcggg tggctggatg acgtcgagcg gcatcgagcg
     1381 gcacgccttg ttcgtgaaga cacacggcag cgctacatgc tgactcacgg aggccttagg
     1441 gcggtgctga gtaggtatct cggcgttaac ccggatgtgg tcgcgctgga tcgcagtgcg
     1501 acaggcaaac ctttcctaaa aaatgaacta cgagatcggt tgccgatcac gttcaatctg
     1561 tcccatgccc atgaccgtgc gctcattgca atctcaaaag ggcaggaggt cggagttgat
     1621 cttgaattcg ttcgtgcgga tatcgaggtt gcgaatctat cccagcgctt ttttactccc
     1681 tccgaacatg cggcgatcat gcaggcgacg gaggaacggc gcgccacgag attcttccgc
     1741 tactgggtcg ccaaagaggc gctgctcaag gcgcaaggta tcggcctcgg gggactgccc
     1801 gactgcgaga ttgtttttga ggcggacggg gttgacacag acgttcgggc ccgattgggt
     1861 gctcggttcc caaatacgtt gcgggttcgg cttctccctt gcgaaaaggg atgggaagca
     1921 gcggtggctg ctcagaggct gggcgcggtt aaacagtgcg ggctcgacag cctgtaatcg
     1981 cgctctgttg agttcaagtt tccaccggat cccgcaccgg ccccatcagc gactgcactc
     2041 aggcgaatgc cggacgcatt gccgaagcca agagccgatt tgatcgagca acccggcaat
     2101 cgctcggttg gcagcctgga cacccccata gggattctcg ctcgtggcgt tctccacgac
     2161 ctcaaaagct cgcgtgctca ggatcgtcga ccctttcaac tcgacgagct gcgcccgaac
     2221 cattacccgc acgcgactgg gttgttggag aaattcttgc tgtaacagaa atccgtgggt
     2281 gtcaagacga tagtctccga gaatcgagct cggcagtgga atcacggcac gccatgcatc
     2341 attctgctga ttgagcgcct ggaccaccaa cggggcgaac atgcgcaccg gcatatcggc
     2401 ccattgattc accgcatagt actccagttc gtggggccgc ttgacataca ccatgcgctg
     2461 cgtttcgaat ccgggctcgg cttgaggcgg gctcacgagc agtacaggac cgtcaagctt
     2521 gccgggctgc gcctcgctgg gccacccgtc aagactcaat tgatacgtat ggatcttcga
     2581 agaatccgtg cggggcgaca ggcaaccggc cgccgtcaac accaacagcg cacaggccac
     2641 atgtgccagt cgatagttca tcatgtcatg accgtacaca ccttgctgtt attcccccgg
     2701 tccgcgtgac ggggccttcc gcccaaacac cagcgagctc ggctctcgct ccacttcgcg
     2761 cgccacacga gtcaatgtac ccgtgagctg tcgaagctcc gtcaccaaca agccggcctc
     2821 gggaagagtc cgtctggtga actgttgcag ttcgggtctg gcttcgttga cgaccgtgcc
     2881 gacggtcttg ctcgtgcgag ccagttcgtc cgtcgccgtg cccagcgccg caacactttt
     2941 gttgattccg gcgagtaagg tcggaacctc cgcgttcaac gccgccgtca gcttgacaag
     3001 attgtcggcg ctcctggccg caccgttcaa gctttgctcg atctgagtct tgtgggccgc
     3061 aatggtctgg gtcacatccg agagatcctt gatcgttcgc ttcagcatgg tgcgattgtc
     3121 ttcgtccaga accttcgccg ctccttttgc cgccgtatcg agatcgacca atagttgagc
     3181 caggccttct tctgaaagta atcgggagac ggcttcatcc aagcggaaga ggagagaggg
     3241 accggtcttg atgatcggat atatttgtcc ctccagggcc tgcagcgagg gagcatctcg
     3301 gcttcctccc gtcaaattga ttgtggctag accggtgagc ccctgcgtct cgagtacggc
     3361 aatcgtgtcg gtcttgatcg gcgtcccgtg cataatctcc atggtcagca gaacttcctc
     3421 cgggttattg ggattcaacg caatggtctt gacccgaccc acgtcgacac ctcgatattt
     3481 gacggtggaa tcgacgctca atcccgcgac cgactccctc atgtaggctt cgtaccgatc
     3541 gtaagcgcct cggtagtcgg tcttcccgag ccacaaaatt ccaaccagga cggccgcgcc
     3601 aagaaacgcc acgaacgagc cgaccagaat atagttgacc ttgggttcca tgtttaatct
     3661 cgctcagaaa agcgaatagc ttatagcgta tggcttatgg caaaaagcga agccggacaa
     3721 tgagttcgta cccctcgttc gtgctaccag ccatgagccg tccgctccta ttcgacgctt
     3781 cacgtttcac gcctttcctg gttccatacc gcctgttcgc tcgctgccct tccgcgtgga
     3841 ccttggaagt attcacggat gacaggatcg tccgatcgag acaactcctg catcgtgccg
     3901 atgcccaaca cttttccacg accgagcacc gccactcgat tggcgatccg ccacagcgaa
     3961 tccaggtcgt gtgtcaccat caccaccgtc agtccaagga gatccttcaa cgagaggacg
     4021 agatcatcga atcctgcggc gatgatcgga tcgagcccgg ccgtcggctc atcgagaaac
     4081 aataattccg ggtccatgac gatggctcgg gcgagcgcgg cccgtctccg catccctccg
     4141 ctgagttcgt tggggtattt cgtggcactg tccggcggta atcccaccat cgcaattttg
     4201 gcagcaacga tgtcgcggat caatccgtcg ctcagtgtcg tatgttcgcg caagggcacg
     4261 gcgacattct ccgccagcgt aaacgaacta aacagcgcgc catgctggaa catcacaccg
     4321 aatcggcgat ggattggatg gctatcaccc gtttccaatt gtcggctgtc tattccgaac
     4381 agccgtatcc ttcccgccga gggtgtgatc aggccgatga tctcccgcaa caacgtggtc
     4441 ttgccgcacc cgttgccgcc cgcaatggcg aagatttcgc cccgccgaat agacagactg
     4501 acgtcctcat gcacgacagc ctgtccaaac ttcgtcgcga cgtggctcac atcgatcacc
     4561 ggtgtttcaa tgttcgtggc agacggcatc agggactacc ccccacacaa gctacaggtc
     4621 ccaccaattg agcaagatgc tgcagatcgc gtcgaacacg atcaccaaaa aaatcccctg
     4681 cacgacactg atggtggtgt gtcggcccac atcgtccaca ccgcctctga tctgaaatcc
     4741 ctgatagcag cccaccaacg cgatgatcgc cgcgaagaac ggcgccttcc ctaagccgat
     4801 gagaaaatgc cgcaccggaa cggcttcttc gaatcgagcg ataaattcta caaagctcac
     4861 gttgagctct cccaaggcaa tcagcatgcc gccgaacacg cccaccacgt cggcatacac
     4921 cgttaacaga gggagggcga tgatcaaggc cagcgctcga ggcaacacga gcagattcat
     4981 gggcgagatg cccaatgtcc gcactgcgtc caattcttcc gtcaccttca tggtgccgat
     5041 ctcagccgca taggcggatc ccgaacgacc ggcgatgagg atggccacga tcaacggggc
     5101 gatttcccgc aacagcgaga tgccgaccag atccacgata aaaatgttcg tgccgaactt
     5161 acggagctgt tcagcgccct gatacgcaat gacgacaccg acaagaaacg tcagcagacc
     5221 ggtgatcggc aaggcccgca cgccgtccag gtccacgatc cgcaagagcg tccgccatcg
     5281 aatggaatgt ggtcgtatca gcgctcggct gaatgcgatg gtgctttctc cgacgaatgc
     5341 gagcgcgcga atcgcggaca cttgccgaga ctgcacgaca cgcacgagac cgtccatcca
     5401 cctcgtaccg accgagaccc ctcgctcggg ccgggaccac tgaacttcta ctttccggag
     5461 caattcggca aattcaggtt tcaatccttg gagggtggtc tgctgtccgt tgcgtcgcag
     5521 gccgtcgatg cagcgctgca tcatgagcgc gccgcccgta tccatagcgg tcacttcccc
     5581 ggcatcgtag aggacggagg gggtatcagg ccatcgaagc gctcggcctc cccgctccag
     5641 ttgagacaag tgcggaagag tccaagaccc tcggcagaga atcacattat ctttgataga
     5701 gatggttgcc tgttccatct gattcactcc gtcggacaca ggggctcttc acgtgatcga
     5761 gcacaccctc gcacaatcat cgccgacgat cgcaaggaag gtcgtcatgc tcggttcacg
     5821 ccgcctctag gatggctttg agctccgcac cggtcattac ttctcgttcc aacaataact
     5881 gtgctccttg tcgcagagca gctttccgat cttccagcaa tcgcctcacc cgtccgtact
     5941 gctcgtcaac gatccgccgc acttcgcaat cgatttcacg ggcggtctct tcggaataat
     6001 cgcccttttc agacgcgacc ggaagctgaa ggaattgagg ctgtcgctcg cgttccaacg
     6061 tgatcgtgcc aagtttctcg ctcattccgt aggccttcac catgcttttt gcgatgtccg
     6121 tggccttgac gagatcgttc tgcgctcccg tggaggcttc cccgaagatc gtttcttcag
     6181 caatccgccc gccgagtaac acagcgacct tattctccaa ttccgatttc gtcattagaa
     6241 accgatcctc cgtcggaagc tgcaacgtat agccgagcgc cgccacgccg cggggaatga
     6301 tggagatttt ctgcacctga tccatccccg gcaaagacat ggcgatgagt gcatggcccg
     6361 tctcatggta ggccacccgc tccttctcca tcttattcag gacacgattt ttcttttcca
     6421 agcctgcgat gactcgctcg accgcctcct gtaattcaga aataccgacc tgatccttgc
     6481 cacggcgcac cgccagcaac gccgcctcat tgatgatgtt ggccaaatcg gctccggaga
     6541 acccgggggt catcgctgcg atgttttcga gatcggcgtc gggaccaagc gccacctgtt
     6601 tggcatgcac acgaagaatg gcgaggcgac cgatcttgtc cgggcggtcg accaccacat
     6661 gacgatcgaa ccggccggcc cgaagcaacg cagggtcaag aatctcgggg cgattggtcg
     6721 ccgccatgag aatgaccccg atgcgaggat cgaagccatc catctcgacc agcaactgat
     6781 tgagcgtttg ctcccgctcc tcatgcccca tgggccccga gccacgcgct tttccgagcg
     6841 catccagttc atcaatgaaa ataatgcagg gagctttgcc ctttgcttgt tcgaacagat
     6901 cacgaactcg cgccgcgccg accccgacga acatttcgac gaactccgag ccgctgatgc
     6961 tgaagaacgt cacaccggcc tcaccggcca cggcacgtgc cagcagcgtt ttgccggtcc
     7021 cgggcggtcc gacgag
//
LOCUS       DATMTI010000299         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_265391, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000299 DATMTI010000000
VERSION     DATMTI010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265391"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2407
                     /locus_tag="VN039_05035"
     CDS             <1..2407
                     /locus_tag="VN039_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HWV45386.1"
                     /translation="YPPDEWNLIEKRFMPALLEQGETMFALGNGYLGMRGCFEEGGPI
                     GQNGTFINGFYESWPIVYPEEAYGLATTGQTIINATDTKIIKLYVDDEPFWLPHANVR
                     SFTRRLNMQSGTLDRDILWETPAGKRVSITSRRLVSFEHRHLAAIFYQVTMLDAAAPV
                     VLSAEMIANEPVRRRDNHDPRQSKVFVEKVLHHRISYSKDRRIVLCHATNKSGMILSC
                     AIDHALDTSCEATWTTHYTEDAGQAVATIDAKPGRPITLVKYMAYHTSHTAPPEEMCA
                     RVERTLDRAVADGFPALLAGQERYMGDFWQRSDINVATNPVRAKASTTAVQQAIRFNL
                     FHILQAAGRAGNMGVPAKGLTGDAYEGHYFWDSEIYVLPFLIYTAPRIAKNLLRFRHG
                     MLDKARERAKELNQRGAMYPWRTINGEEASAYYAAGTAQYHINADIMYALRKYVQATG
                     DEEFLFDEGVEMLVETARLWLSLGFYSLRQGGQFCIHCVTGPDEYSVMVNNNVYTNLM
                     ARENLRYAAETVEKVRERRPDLFTALVHKTRLEASEVEDWKRAADLMYVPYDERLKIT
                     PQDDKFLEEEPWDFKHVPPEKYPLLLYYHPLTIYRYQLIKQADLVLAMLLLGHEFSPE
                     LKRRNFEFYDPITTGDSSLSPCIQSIMAAEVGDMAKALEYGKVATLMDLGDVAGNVKD
                     GCHIAAMGGVWMVFVYGLAGLRDYTGRLSFRPALPEQFDCLRFRLAFQGQSLEIDVSQ
                     DATRYTLIAGNGLTLNHEDQEIRLSPAAPSVVGPNRKLSKREGTNHVILTDGPWMEQQ
                     ELS"
     gene            complement(2419..3228)
                     /gene="gntA"
                     /locus_tag="VN039_05040"
     CDS             complement(2419..3228)
                     /gene="gntA"
                     /locus_tag="VN039_05040"
                     /inference="COORDINATES: protein motif:HMM:NF041366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis heme-dependent
                     pre-guanitoxin N-hydroxylase GntA"
                     /protein_id="HWV45387.1"
                     /translation="MSVNMHTGMGNARQWNPLSIDPAASRFSSYAAFNGEAITRPFAP
                     EIPIDTELIDAHTRLRRQILSRFYPCTGAISAFSQKNYRFGLYPELACHSAVRAVCHD
                     LYCFCHEFSSIGDQFITFVAMFRGPAIQSERHFEDLLWNQLQGMHALDSDSFTWDKDV
                     ESDPASRHFSYSIGGRSMFVIGMHPKASRLARTRPYPTLVFNLHEQFDRLRSRGKFET
                     MKQTIRAREMAFQGSINPMLSGFGENSEARQYSGRAVSPNWVCPFHKKAGA"
     gene            complement(3242..>3926)
                     /locus_tag="VN039_05045"
     CDS             complement(3242..>3926)
                     /locus_tag="VN039_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45388.1"
                     /translation="LQGSQKVFGRVEAVTSDQIKVDIGEMQPRFLPLKQAEEKNFPQI
                     KPGDDLVIIVNGQNLIVDYHPVDHPSGGHKIVRGAMADTMPVGHERVVIKSKDGQEQS
                     FEVRSQARSKLASIPVGTPMLFLIDETNKVTDATFASIDAAKEANKRPEDRSSIKGAN
                     RQVGGTVVEPLSGDRITIKTGDGERPFEVREIMQERLSSLRKGESVILLMDNEDKVVD
                     IAVPPASAR"
BASE COUNT          883 a         1098 c         1106 g          839 t
ORIGIN      
        1 ctatcctccg gatgagtgga acctcatcga gaaacggttt atgcccgccc tgcttgagca
       61 aggtgaaacc atgtttgcgc tcggaaacgg ctatctcggt atgcgagggt gtttcgagga
      121 aggaggaccg atcggtcaga acggcacctt catcaacggc ttttacgaga gctggcctat
      181 cgtatacccc gaagaggcct acggcttggc aacgacaggg caaacgatca tcaatgcgac
      241 cgacaccaag atcatcaaac tatacgtgga cgatgaaccg ttttggcttc cccacgccaa
      301 tgtcaggagt ttcactcgac gattgaatat gcagtccgga acactggacc gcgacattct
      361 gtgggaaacc cctgcgggga aacgggtctc gatcacatcg cgccggctcg tgtccttcga
      421 gcaccgccat ctggccgcca ttttctacca ggtgacgatg ctggatgcgg ccgctccggt
      481 cgtgctctcc gccgaaatga tcgcgaatga gcccgtacga aggcgagaca accatgatcc
      541 caggcaatcg aaagtatttg tcgagaaagt cctccatcac cggatcagct attcgaaaga
      601 ccggcggatc gtcctgtgcc atgccacgaa caaaagcggg atgatactct cctgcgcgat
      661 cgatcatgct ctggacactt cctgtgaggc gacgtggacg actcattaca cggaagacgc
      721 gggtcaagcg gtcgcgacca tcgatgcgaa gcccggccgg cccatcaccc tggtgaagta
      781 catggcctat catacgagcc acacggcgcc tcctgaggaa atgtgcgcgc gcgtggagcg
      841 gacgctggac cgggcggtcg cagacggatt tcctgctctg cttgccggcc aagagcgata
      901 catgggtgat ttctggcaac gaagcgacat caacgttgcg accaatccgg tgcgagccaa
      961 ggcctccacg accgcagtcc agcaggcgat cagattcaat ctgttccaca ttctgcaagc
     1021 cgcaggtcgc gccgggaaca tgggggtgcc cgccaaaggt ctgacggggg acgcctacga
     1081 aggtcattac ttctgggaca gcgaaatcta tgtgttgccg tttttgatct acaccgcgcc
     1141 gcggatcgcc aaaaatttgc tgcggttccg gcatggcatg ctggataaag cgcgcgaacg
     1201 ggcgaaggag ttgaatcaac gaggggccat gtacccgtgg aggacgatca acggcgagga
     1261 ggcatcggcg tattatgccg cagggaccgc gcagtatcac atcaatgccg acattatgta
     1321 cgcgctgaga aagtacgtgc aggcgaccgg cgacgaggaa tttcttttcg atgaaggggt
     1381 ggagatgttg gtcgaaaccg ccagactatg gctgtcgctc ggtttctact cgctgcgcca
     1441 aggaggacaa ttctgcattc actgcgtgac cggtcccgat gaatacagcg tgatggtcaa
     1501 taacaatgtt tataccaatc tgatggcccg cgaaaatctc cgatacgccg ccgagacggt
     1561 cgaaaaggtg cgggaacgcc ggccggattt attcacggcg ctggtgcata aaacccggct
     1621 ggaggcctcg gaggtggagg attggaagcg ggctgccgac ctcatgtatg tgccttacga
     1681 tgaacgtctg aagatcactc cgcaagacga taagtttttg gaagaagaac cgtgggactt
     1741 caaacacgtg ccgccggaga agtatccgtt gctgctctac tatcatccgc tcaccatcta
     1801 tcgatatcag ctcatcaaac aggccgatct cgtgctggcc atgctgctgc tcggccatga
     1861 attctccccg gagctgaagc gacgaaactt cgaattttat gatcccatca ccaccggaga
     1921 ctcgtcactc tcaccctgca tccaaagcat catggccgcc gaggtcggcg atatggcgaa
     1981 ggccctggag tatgggaaag tggcgacatt gatggatttg ggcgatgtcg cgggaaatgt
     2041 caaagacggc tgccatattg ccgccatggg cggagtgtgg atggtgttcg tctatgggct
     2101 tgcgggattg cgcgactata ccggtagact gagcttccgg ccggccttgc ctgaacagtt
     2161 cgactgtctc cgattcaggc ttgcgtttca aggacagagt ctcgagatcg atgtcagcca
     2221 agatgccact cgctatacgt tgatagccgg caacggactg acgttgaatc acgaagacca
     2281 agaaattcgg ctctcgcccg ctgctccgtc cgttgtagga ccgaaccgga aactctcgaa
     2341 acgcgaggga acgaaccacg tgatcctcac cgacgggcct tggatggaac agcaggagtt
     2401 atcctagttt tgcagtgatc atgctccggc cttcttgtgg aaggggcata cccaattagg
     2461 cgaaacggcc cggccggaat attgacgtgc ctccgagttt tccccaaagc cggacagcat
     2521 gggattgatc gaaccctgga acgccatctc gcgcgcccgt atcgtctgtt tcatcgtctc
     2581 gaactttccg cgcgagcgca gacggtcaaa ctgttcatgc aagttaaaca ccagggtcgg
     2641 atacggacgg gtccgcgcca gacgcgaggc cttgggatgc atcccgatga cgaacatgga
     2701 tcgacctcca atgctgtaag aaaagtgccg gctggcaggg tccgattcca cgtctttatc
     2761 ccatgtgaag gaatcagaat ccagagcatg cattccctga agttgattcc atagcagatc
     2821 ttcgaagtgc cgctccgact ggattgccgg tccgcggaac atcgcgacga aagtaatgaa
     2881 ctggtcgccg atggacgaga actcatgaca aaaacagtac aggtcatgac acacggcacg
     2941 tacggcgctg tggcaagcca gttcgggata aagtccaaaa cgatagtttt tttggctgaa
     3001 agccgagatt gcaccggtgc acgggtagaa ccggctcaag atctgccgtc gcagccgggt
     3061 atgtgcatcg atgagttcgg tgtcgatcgg tatctccggt gcgaacggtc tcgtaattgc
     3121 ctccccattg aaagccgcat agctcgagaa ccggcttgcc gccggatcga tcgataaggg
     3181 gttccactgg cgcgcattgc ccatcccggt gtgcatattc actgacatat ggctaatcca
     3241 attaccgagc ggatgccggt gggacggcaa tgtccaccac tttgtcctca ttgtccatga
     3301 gaagaatgac tgattctccc ttgcgaagac tagagagccg ttcctgcatg atttcacgaa
     3361 cttcgaacgg ccgttctcca tcgccggtct taatagtgat acgatcgcca ctcaacggct
     3421 cgacgaccgt cccgcccact tgccggttgg ctcccttgat cgacgacctg tcttccgggc
     3481 gcttgttcgc ctccttcgct gcatctatgc tggcaaacgt cgcgtcggtt actttgttcg
     3541 tttcatcgat taggaagagc atcggtgtcc cgaccgggat ggaggccagt ttgcttcgcg
     3601 cctgagaccg cacctcaaac gattgttcct gtccgtcttt gctcttaatg accacccgct
     3661 catgccctac cggcatagtg tctgccatgg cgcctcggac tattttgtgg ccgccggacg
     3721 gatgatccac cggatgataa tcgacgatca gattctggcc gttgacgata atgaccaaat
     3781 catcacccgg cttgatctga gggaagttct tttcctcagc ctgtttcaag ggaagaaatc
     3841 ggggctgcat ctcgccgatg tcgaccttaa tctgatccga tgtgacggct tccaccctgc
     3901 cgaagacctt ttgactcccc tgcaag
//
LOCUS       DATMTI010000300         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_181327, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000300 DATMTI010000000
VERSION     DATMTI010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181327"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..980)
                     /locus_tag="VN039_05050"
     CDS             complement(333..980)
                     /locus_tag="VN039_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45389.1"
                     /translation="MTLLKQVTSLLGLIGVLTAGTSGVWAIDVNLSSDEAHKALEIGR
                     IPMEKTNSPEDVKKVLQQASLATRVGADPEKDPCGASAVLRTKRYRLEAFGRQEAAES
                     KKRKTDVRMPEEFIQKVMDMPNMELEVQLCGDDEYFAEGALIELQQGSKRIKPIDIGK
                     AERGRKNESNGPAFRSRFTALFAYEQFDPTASSVFVVNLQDGTEIRISADLSKVK"
     gene            complement(1077..1709)
                     /locus_tag="VN039_05055"
     CDS             complement(1077..1709)
                     /locus_tag="VN039_05055"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWV45390.1"
                     /translation="MQLVLASNSPRRRELLTLLGLSFEVCPSEFHEQPTVGLSPVEQV
                     RRFAREKATSVALVRPQALVLGSDTVIDLEGRLLGKPVDLADARAMLARLAGRSHHVH
                     TAVALCDRVRHIESVEVATAEVRMKADLDQAYERYLASEESFGKAGAYAIQGLGGDLV
                     ERIIGDYTTVVGLPLKLVAQLLRSADYPLLVNVEDLYRRKPYTNWNRFTT"
     gene            complement(1700..>2795)
                     /locus_tag="VN039_05060"
     CDS             complement(1700..>2795)
                     /locus_tag="VN039_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HWV45391.1"
                     /translation="VIAQQGKPDVSFTEFTHVHDVCHGPEIHLETLLYSEKERPIVAQ
                     LYGKDPDLFYLAALAVCELGFDGLDINMGCPSKSVASSGSGAGLIRTPELARTIMQAA
                     KRGIEDWADGRTLQQAGFRPARVAMFERLNRQRGKTGPTHRRQVPLSVKTRLGYDSVT
                     VEGWIEELLMEQPAVISLHGRTLRQMYRGSADWSAIGRAATLVRGTGTLLLGNGDIQN
                     LGDIAVRVRETGVDGVLVGRGVLGAPWFFRSKEQARMRARGMGGADVGRDPGEVSLNE
                     RFAVLVDHAQQFQALVGEKQFYRMRKHLGWYCKGFPHAASLRAQMVQVSSVEALHTLL
                     ADFHDRPEAITDHHQAESADELSMLVSRCS"
BASE COUNT          574 a          853 c          748 g          620 t
ORIGIN      
        1 agagcggcga tgtattacgc tcgaagaact tctggtctac tttcccgagc tcacctggaa
       61 tcaagtattc tcgctcgtgg atgagctcag tcgtcacgcg ttgattggtt tgcgacggcg
      121 tggatttgaa tatgaattgc gagcgctctc ctgattcgcc gagcggcatg ctctctcttt
      181 gagactgcct cggcaacgtg aactgccgaa gattaaacgg gatgctccgg ggcaggcatc
      241 ttcagcttcg aagcagaccc tcctctcatt gattctgggg actcgttctc tcccggtctg
      301 gttgggcgtc cagagccagc cgcagagaga acttacttca ctttagagag atcggctgaa
      361 attctgatct ctgttccgtc ttgcagattg acgacgaaca ccgacgatgc ggtggggtcg
      421 aactgttcgt aggcgaagag cgccgtgaac cgtgacctga aggcaggacc attgctttcg
      481 ttctttcgcc cccgttcggc cttgccgatg tcgataggct tgatccgctt cgacccttgc
      541 tgcaattcga tcaatgcgcc ctcggcgaaa tactcgtcgt cgccgcacaa ctgcacttcc
      601 aattccatgt tgggcatgtc catcactttc tggatgaact cctcgggcat gcgcacatct
      661 gtctttcgtt ttttcgattc tgccgcctct tggcgaccaa aggcttcgag tcgataccgc
      721 ttggtgcgaa ggacagcgct cgcgccgcag gggtcttttt ccggatctgc acctacgcgg
      781 gtcgctaagg acgcctgctg aaggactttc ttcacgtctt ccggcgaatt ggtcttttcc
      841 atcggaattc gaccaatctc cagcgctttg tgcgcttcgt ctgacgacag attgacatca
      901 atcgcccaca cgccgctggt ccctgctgta aggactccga tcaggcccag cagtgacgtg
      961 acctgcttga gaagagtcat gccttcctct tatagcaccg acatggcgaa gtctaaagta
     1021 tccgatacaa tcagatgaat ggaattcatt gtaggtgaac catcgtgcct ttgcaattag
     1081 gtcgtgaacc gattccaatt cgtatacggc ttgcgtcgat acaggtcttc gacgttcacg
     1141 agaagaggat aatcggctga ccgaagcagc tgcgccacaa gcttcagcgg taaccccacc
     1201 acggtcgtgt agtcgccgat gatccgttcc acgagatctc caccgagtcc ttggatcgca
     1261 taggctccgg ctttgccaaa cgattcctcc gatgcaagat accgttcata cgcttgatcg
     1321 agatccgctt tcatccggac ttccgccgtg gcaacctcca ctgattcaat atgtcgcact
     1381 cggtcgcata aggcgacggc agtatggaca tgatgggaac ggccggccag acgtgccagc
     1441 atggcgcgtg catctgcaag atccaccggc ttcccgagta gtcggccctc gagatcgatc
     1501 acggtatcgc tgccgagcac gagggcctgt ggtcttacca gtgccacgga tgtcgccttt
     1561 tcacgcgcga aacgcctgac ctgctcgaca ggcgacaacc cgaccgtggg ctgctcgtga
     1621 aactccgacg ggcacacctc gaaggaaagg cccaacagtg tcagaagttc tcggcggcgt
     1681 ggcgaattcg acgccagcac tagctgcatc gcgagaccag catgctcagc tcatcggctg
     1741 attcggcctg atggtggtcc gttatggctt ccggccgatc gtggaaatct gcgagaaggg
     1801 tatggagtgc ttcgacggaa gagacctgca ccatctgtgc gcgaagcgag gcggcatgtg
     1861 gaaagccttt acaataccag cctagatgtt tacgcatacg atagaattgc ttttctccga
     1921 ccagcgcttg gaactgttgc gcatgatcga ccagcacggc gaagcgctcg ttcagcgaaa
     1981 cctcgccggg atctcgcccg acgtcagccc cacccatacc acgggctcgc atgcgggcct
     2041 gttcttttga gcgaaagaac cacggcgcgc caagcacacc ccgcccgacc aacacaccat
     2101 cgaccccggt ttcacgaact cgtaccgcga tatcgccaag attctgaatg tccccattcc
     2161 ccaataacaa tgttccggtt ccccgcacca gggtggcagc gcgcccaatg gccgaccagt
     2221 ccgccgagcc tcgatacatt tgccggagcg ttcggccatg aagcgagatc acggccggtt
     2281 gctccatcaa aagctcctcg atccatccct caaccgtcac tgagtcataa ccaagtctcg
     2341 tcttcacgga aaggggtact tggcgccgat gggttgggcc ggttttgccg cgctgacgat
     2401 tcagacgttc aaacatggcg actcgcgccg gtcgaaaacc cgcttgttga agggtccgcc
     2461 catcggccca atcctcgatc ccacgtttgg cggcctgcat gatcgtgcgg gccagttctg
     2521 gagtgcggat caatccggcg cctgatccgg acgacgccac actcttcgac ggacaaccca
     2581 tgttgatgtc cagcccgtcg aaacccagct cgcacaccgc cagcgccgcc aaataaaaga
     2641 gatccgggtc tttcccatag agctgcgcca cgattgggcg ctccttctcg ctgtacagga
     2701 gcgtctccaa gtgaatctcc ggtccgtgac agacgtcatg gacgtgcgta aattctgtaa
     2761 aactgacgtc cggcttcccc tgctgggcga tcacg
//
LOCUS       DATMTI010000301         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_181330, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000301 DATMTI010000000
VERSION     DATMTI010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181330"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1033)
                     /locus_tag="VN039_05065"
     CDS             complement(143..1033)
                     /locus_tag="VN039_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45392.1"
                     /translation="MEIGATVEERLTEKQCWELFQRLFPNGFDDPDLVQHLAPEGWER
                     SPLMRVYHPTAEQVYEETLRLRDHLKRLRGEAALPEDEPQISLDSLRREMPAEAPKPV
                     EEFADLLGSCLWDIFSDNHEVRTADALVDLGTFRAAAGFIADIRHCQNNLEVGFSRRD
                     GYLEFYMGTSMVRHRADLTQVYELIFCRMRQFGLDWRYVHPRLMAVDLRPLQEASESA
                     KIPAVVRYNPTENFWREREEAAHDAELAELRRNLSEGYRESVEEARNAPPPGTVRAYR
                     RVYGRFPVGWPPQADGENDG"
     gene            complement(1151..1597)
                     /locus_tag="VN039_05070"
     CDS             complement(1151..1597)
                     /locus_tag="VN039_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWV45393.1"
                     /translation="MNRSRPIRIGVIADTHGLFDSTIARHFQGVDHIIHAGDIGKRSV
                     IEQLEAIAPVTAVSGNVDEYEKSGFPTETIIQLNGLRIAIRHVVFEAGKLTKAGRAFL
                     DRTRPDICIFGHTHQPKNGWLGDTLLFNPGSAGPKRFRLSRSWVSS"
     gene            complement(1594..>2855)
                     /locus_tag="VN039_05075"
     CDS             complement(1594..>2855)
                     /locus_tag="VN039_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWV45394.1"
                     /translation="LNVRQRVRLRLFLTCGLVGRRFEHPQENIPPSIENHAMTESHLN
                     ALCFGEEFPANGMPCLVHVEGHGLTMTFLSDREAGERRSESVPFSELTVSAGGLDHDQ
                     LVATWTGPTGQRTLYLKNPDVIRAFRQAAPDHLTIPLERAAEQVRRVRQWHRIVWGIV
                     GGSLLASVLGLWFGSDLLVELAVDRIPIEWERKLGESAYHDFLARQEVMKEGPAVTAV
                     TEMTHRLTDPIPENPYTFEITVVKSDVVNAFALPGGYIVVFTGLMKKAESPAEVAGVL
                     AHELNHVLQRHGLERIVKQLGFVAVVSIVLGNQQGLGGVMKQLGVELMTLKFGRAQET
                     EADLTGLQLLHRAKIDPSGMITFFQRLAEKDEGRVEWFSTHPMSSARADRLKAELADM
                     PKQTPEPFTFDWTKVRASLGAHTGGSP"
BASE COUNT          624 a          867 c          749 g          615 t
ORIGIN      
        1 cgcattggag caaggcagcg ttgtggtttt tgaagagagc cgcgttcgga ttcgcccttt
       61 accgattggg cgtcaacaat gacaagttcg atcgagcagg cttcgcacgt tggggagcag
      121 ttccctcggt gaactcctta agttaaccat cgttctcacc atcggcttgg ggaggccagc
      181 caactgggaa acggccgtat acccgccgat acgctcgtac cgtgcccggg ggcggcgcgt
      241 ttcgagcttc ctccaccgat tccctatagc cctcgctcag atttcgccta agctcggcca
      301 actcggcgtc gtgcgccgct tcttctcttt cacgccagaa gttttccgtt ggattatacc
      361 gaaccactgc aggaatcttc gcactctccg atgcctcctg aagcggacga aggtccacgg
      421 ccataagcct cggatgcaca taccgccaat ctagaccgaa ttgccgcatc cgacaaaaga
      481 tcagctcgta aacctgagtc agatccgcgc gatgtcggac catagaagtt cccatataga
      541 attcgaggta accgtcgcgc ctggagaacc ccacctcgag gttgttttgg caatgtcgta
      601 tgtccgcgat gaagcctgcg gcagcgcgga aggttccaag atcgacgagg gcatcagcgg
      661 tgcgaacctc atgattgtcg gagaatatat cccacaaaca ggagcctaac aggtcggcaa
      721 actcctcaac cggcttcggc gcctcagcag gcatctctcg acggagactg tcgaggctaa
      781 tctgcggttc atcctccggc agcgccgcct cccccctcaa tcgcttgaga tgatcacgca
      841 accgcaacgt ctcttcgtac acctgttccg cagtcggatg atagacgcgc atgagcggag
      901 aacgctccca tccctctggg gctaagtgct gcaccaggtc tggatcatcg aacccgtttg
      961 ggaacagacg ctggaacagc tcccaacact gcttttccgt caagcgttct tccacggtgg
     1021 ccccaatctc caagatgctc acacatcttg taggcattct gcagcagacg cgaactgttt
     1081 tctggcatat gaaatttccc tgcacggtcg gcaagtgcga catgggaggc actgacttta
     1141 ccttcactga tcatgaggag acccagctgc gtgacaacct gaagcgcttc ggccccgctg
     1201 atcccggatt gaataagagc gtatcgccca accacccatt cttcggttga tgcgtatgac
     1261 cgaagataca gatgtctggt ctggtacgat cgagaaatgc tcttcccgct ttggtcagct
     1321 ttccagcttc aaataccaca tgccgaatcg cgatgcggag gccattcagt tggatgatgg
     1381 tctctgtcgg gaaaccgctc ttttcatatt catcgacatt gcctgagacg gccgtcacag
     1441 gagcaatcgc ctccagttgt tcgatgaccg atcgtttgcc gatatcaccc gcgtgaataa
     1501 tatgatcgac accctggaaa tgccgagcaa tcgtcgagtc gaagaggccg tgggtatcgg
     1561 caatgacccc gatgcggatc ggtctcgagc gattcatgga ctgccaccgg tgtgagcacc
     1621 aagcgaggct cggactttgg tccaatcgaa tgtaaacggc tcaggggttt gcttcggcat
     1681 gtctgcgagc tcagccttca gacgatcagc tcttgcgctg ctcatcgggt gggtggagaa
     1741 ccactcgact cgtccctcat ctttctcggc caatcgttga aaaaacgtga tcatgccgga
     1801 cggatcgatc ttggcacgat ggagcagttg aagacccgtc agatccgcct ccgtctcctg
     1861 tgcccgtccg aacttcaacg tcatcaattc aacgccaagc tgcttcatga ctcctcccaa
     1921 cccctgctgg ttccccagca cgatcgacac gacggctaca aagcccaact gcttaacgat
     1981 acgttccagt ccatgccgct gcaacacgtg gttcagctca tgggctaata cgccggccac
     2041 ttctgccgga ctttccgctt tcttcatcaa tcccgtgaac acgacgatat acccaccggg
     2101 aagggcaaag gcattcacca cgtcgctttt cacgacggtg atctcgaatg tgtaggggtt
     2161 ctccgggatc gggtcggtca ggcgatgggt catctcggtg acggccgtta cggcggggcc
     2221 ttctttcatg acctcctgcc gagcaagaaa atcatgatag gcagactcgc ccagctttcg
     2281 ctcccattcg atcgggattc gatcgacggc aagttccacc agtagatcac tcccaaacca
     2341 gagccccaac accgaggcca gaagcgaccc tccaacgata ccccacacga tccgatgcca
     2401 ttgccgaacc cggcggacct gttccgccgc tcgttcaagc ggtatggtca agtgatcagg
     2461 cgccgcttgt cggaaggcac gaatcacgtc ggggtttttg agatacagcg tccgctgccc
     2521 tgtcggcccc gtccatgtcg cgacgagctg gtcatgatcc aatccgcctg ccgaaacggt
     2581 cagttctgaa aatggcaccg attccgaacg gcgctcgccc gcttcacgat ccgagagaaa
     2641 agtcatcgtg agcccatgtc cttcgacatg gacaagacac ggcatcccat tggcagggaa
     2701 ttcctcacca aaacagagcg cattgaggtg ggattcggtc atggcgtggt tttcgattga
     2761 cggcggaatg ttctcctgcg gatgctcaaa acgtcggcca acaaggccgc aggtgagaaa
     2821 caaccgcagg cgtaccctct ggcgtacgtt gagga
//
LOCUS       DATMTI010000302         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_138359, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000302 DATMTI010000000
VERSION     DATMTI010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138359"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(921..1409)
                     /locus_tag="VN039_05080"
     CDS             complement(921..1409)
                     /locus_tag="VN039_05080"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HWV45395.1"
                     /translation="MELTKAPFDATTALTDGTSKAVGELLEPTKKFVSSTTPGAATID
                     RFTRAREKTELFVGYSYEHLRAESARGSGEHIASLAVLAGIPSDRRPQFQVKMRDTYS
                     TMFNESLPTREARLRIVNAAWSEGYGRLESDTITHQETGISDIDLKFSKSRKSTSLMS
                     LR"
BASE COUNT          426 a          444 c          464 g          444 t
ORIGIN      
        1 gtttctcggt gcaggctttc tcgacgcgat cactaccttg gcgtacagtc cgatcctgag
       61 tgttaggagt tgaagcacag gcactgagca ataccaggga gaagaaaacg gataggggtt
      121 tgtgatccat agccattaga tccctagtgg attgagggga agttctgtac cacaggccag
      181 gaaccgtgca aggtccacca ctagacacta ggtagtctct aggcttaggg ttatctgaaa
      241 cgtacacacc taaacgagga gcaagcccat gcctggaatg tttggtgctc atcctccgct
      301 catgctgatc gctattgcca ttgtcatgat cgcgattgtg gtgtttgcat tgatgacggc
      361 aggttattag gccggatcag cgaagaatca acggctcatc tgctgctttc ttagtctgaa
      421 tgcgggccga agaacgttgc ggcactgatt ctgcgtgact catcccaaat gggatcgcga
      481 gtatgaatca gagaggggaa cgcgagatgc cacgaaggat agtgaaggta agaatcgcgc
      541 ccattgcgac tattcgcatg cagccacagt ttggatgcta cagagcagtt cctactcctt
      601 gagtaacctc ctgcgaagca ttgcagctac cgcaccggag tatcaggccg acgcggttgt
      661 tcagtgcttc ggcgatcagg acaaggtgat atcgctcgct tccacagagc gcgcacgaat
      721 agccatccag ctgaggtccc gagttctgcc gccgccgctg cttactttgt tgaaccttcg
      781 agacgttgtg aatcccttgc atcaattctc tcctttgtct tgtcgatagt tctgtctatg
      841 ccggtgaccg ataagtcaga agcagttcac ttcagaaatc ctttgctggt ttccatcacc
      901 atcacacaca cagagactga tcagcggagg gacatgagag aagtcgattt ccgagatttg
      961 ctaaacttca ggtcaatatc ggaaatgccg gtctcttggt gcgtaatcgt atcggattcc
     1021 aatcttccat atccttccga ccacgctgca ttcacaatgc gaagcctcgc ttcacgagtc
     1081 gggagggatt cgttaaacat cgtcgaatag gtgtcgcgca ttttcacctg aaattgaggg
     1141 cggcgatccg aaggaattcc tgccaagaca gcgagggaag cgatgtgttc accggatcct
     1201 ctggcgcttt ctgcccgcaa gtgttcatac gaatagccta caaagagctc agtcttctct
     1261 ctcgcccgag tgaagcggtc aattgtggct gctcccggag tagtgctcga tacgaatttt
     1321 tttgtgggct cgagcagttc gcctacggct ttcgatgtgc catcggttaa tgccgttgtc
     1381 gcatcgaatg gagctttggt cagttccacc gtggcttccg ttatgcagcc agataacatc
     1441 agtattacaa aaagaagccc ccatgattgg aatcgttcca taggtgaagc agcctaataa
     1501 ctggtagcgt ggtatttgct gacggttaat ctatatatat ttcttcctgc ccagatccac
     1561 acctaggaaa atccataggc tggataatca agcgcagcct atcgaacgca catttgatca
     1621 aggctatcgt ccagaggagc ggataccgac gatcggcatg gcgcccgagc tggacaacag
     1681 ctatcttcgt gaccaagcgg agaccttgat ggtgtttact attgtgcgat acatcggaag
     1741 gaagaacatg ctgcgtccga ccatatttcc gatgtaga
//
LOCUS       DATMTI010000303         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_774872, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000303 DATMTI010000000
VERSION     DATMTI010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774872"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /locus_tag="VN039_05085"
     CDS             complement(<1..1950)
                     /locus_tag="VN039_05085"
                     /inference="COORDINATES: protein motif:HMM:NF018458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HWV45396.1"
                     /translation="MVSTYLSYDLINRDMKASISRVSQQGLVERQTKYYKENIGNVKS
                     VDEFLDNYQLYSYAMDAFGLGEMTYAKAFMKKVLDSDLNDQNSFANKLTDERYREFAA
                     AFNFSSSTKTVQTDAQLDKMIGLYDTSITNLNSSLAEETRYYKAIIGTVTNADQLLRN
                     DRTRAYIFQVFGIDEKTYSYAHIKGLMTSDVSDPNSYINQEYGAAYNDAVEKLTMKGN
                     IELHAQVTDRITAIDTELAGTGLTNEERTKLEAEKVTRQDQLTQLEAVLPPKDEWEAK
                     LAAIKAEQTTLSNTVTQYNKMAQIANAFEFKNDGTVTAGGAQTADNLKAITDSYIGSA
                     ARVTPTVATLNRDYFESKIGSITTVEELMSDARLVNYIRTAFDLNDLTIVPATIKNIL
                     TSDLSDPNNYIATMGKNDKRYLALKNAFNFLPDGTLAAGTTPQTTTQTATTTSGYMTH
                     YNDADEAADAKALTLFKSSIGKVTSVNDFLGTNAVYTYALKAVGLDPAKVNISDIQRV
                     LTSDLQDKKSYVYTLKDDRYVKLAELFNFTKDGSVGAPILAQSEIELQTMSADYIKKK
                     SAFGTAKDKEAATAEAKYFSGEMQKIKTLSEFLANDRLTKFAMESLGIDPTSVTKEQL
                     QKIFTSNLDDKDSYVNKEMDPVFRRL"
BASE COUNT          388 a          573 c          589 g          563 t
ORIGIN      
        1 gaggcgacgg aagaccggat ccatctcctt gttgacgtag ctgtccttgt catcaagatt
       61 tgacgtgaat atcttctgca gctgttcttt cgtcacgctc gtggggtcaa tgccgagcga
      121 ttccatcgcg aatttggtca ggcggtcatt ggccagaaat tcactgagcg tcttgatctt
      181 ctgcatttcg cccgagaaat atttcgcctc ggccgtggcg gcttccttgt ccttagcagt
      241 accgaaagcc gatttcttct tgatgtagtc ggccgacatg gtctgcagct cgatttcgga
      301 ctgcgccaga atgggtgcac cgaccgaacc gtccttggtg aagttgaaga gctcggcaag
      361 cttcacatac cgatcgtctt tcagcgtata aacgtaactc ttcttatcct gaaggtcgct
      421 ggtcagcacc ctctggatat cggagatgtt caccttggcg ggatcgaggc cgacggcctt
      481 cagcgcatag gtatagactg cattggttcc gaggaaatca ttgacgcttg tgaccttgcc
      541 aatgctgctc ttgaacagcg tgagcgcctt tgcgtcagct gcctcgtccg catcattgta
      601 atgggtcatg taaccgctgg tggtcgtcgc ggtctgtgtc gtcgtctgcg gcgtcgtgcc
      661 ggccgcaagc gtcccgtcgg gcaggaaatt gaacgcgttt ttcagggcga ggtaacgctt
      721 gtcgttcttg cccatggtcg cgatgtaatt gttcggatcg ctgagatcgc ttgtgaggat
      781 gttcttgatc gttgccggca cgatggtcag gtcgttcagg tcgaaggccg ttctgatgta
      841 attgacaagc cgcgcatccg acatcagttc ctctactgtc gtgatggaac cgatcttcga
      901 ttcgaagtag tcgcggttca gcgtcgcgac ggtcggcgtc acacgcgctg cgctgccgat
      961 ataggagtcc gtgatggctt tcagattgtc cgccgtctgt gccccgccgg cagttaccgt
     1021 gccgtcattc ttgaactcga aggcgttagc gatctgcgcc atcttgttat attgggtgac
     1081 ggtgttcgac agcgtcgtct gttccgcctt gatcgccgca agcttcgcct cccactcatc
     1141 ctttggtggc aaaacagcct caagctgggt cagctggtcc tggcgggtca ctttttccgc
     1201 ctcgagtttc gtgcgctcct cgtttgtcag acccgtgccc gcaagttcag tatcgatggc
     1261 cgtaatccgg tcggtgacct gcgcatgcag ttcgatatta cccttcatcg tcagtttttc
     1321 gactgcgtca ttataggccg cgccgtattc ctggttgatg tagctattgg gatcgctgac
     1381 gtcgctcgtc atcaatccct tgatatgggc ataggaatag gtcttctcat cgatgccgaa
     1441 gacctggaag atataggcgc gggtgcggtc gttgcgcagc agctggtcgg catttgtcac
     1501 ggtgccgatg attgccttgt aataacgtgt ctcttccgca aggctgctgt tcagattggt
     1561 gatcgacgta tcataaagcc cgatcatctt gtcgagctgc gcgtccgtct gcacggtctt
     1621 tgtcgaagag ctgaaattga aggcggcggc aaattcccga taacgctcgt cggtcagctt
     1681 gttggcgaag ctgttttggt cgttcaagtc gctgtcgagc acctttttca taaaggcctt
     1741 ggcataggtc atctcgccca gaccgaaggc atccatggcg taggaataga gctggtagtt
     1801 atcgaggaat tcatcgacgc ttttcacatt gccgatgttt tccttgtaat atttcgtctg
     1861 ccgctccacg agcccctgct gcgacacgcg cgagatgctt gccttcatgt cgcggttgat
     1921 gaggtcgtag ctgagataag tcgataccat ccgtatgccc cctggagcac agcgcatccc
     1981 ctcggggaca ccaaaagctc cggttttgaa ccacccatcg cgcgcccatc cggacgcacg
     2041 gcaagccttg gcatgactat gcatgacgcg ccttgcccgt agcttgaaag agcacaattc
     2101 cgatcgggat aaa
//
LOCUS       DATMTI010000304         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_138469, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000304 DATMTI010000000
VERSION     DATMTI010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138469"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1955)
                     /gene="pilQ"
                     /locus_tag="VN039_05090"
     CDS             complement(<1..1955)
                     /gene="pilQ"
                     /locus_tag="VN039_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HWV45397.1"
                     /translation="MKPLFLRADNSLTAGCFIGALGLSLAALAIFAETPAFAEEIGMT
                     KRGEATTSRLLAQAVTAIEVRPETDLVTVVVAGDGQLFPEANFLDESRLIVDIPAVSS
                     ALRRSVIRADHYLLKKIRVGHHADKVRLVFDVPERPIFSLAREENKVLITLKPSEHKI
                     PTVVAAQFDGERERVLFTPRARKMLFESGDRVGKRMMRMVGPTQSQFKVQRVQMAGDS
                     VPVEKADRSDDIVAGQTRYVGRRISLDFQQADITNILRLIAEVSGFNIVVGEGVKSKV
                     TMKLVSVPWDQALDMLLKMNGLGMIRQGSIVWVDSLANIAKQQDEEARAKDSKTKAEE
                     LIDRVFYIRNLQAQEVMTSLRQYLSPRGVMQFNQGSNALIVRETESKLAIVKQLVEGL
                     DLEVPQVQIEARIVQADTVYARGLGIQWGFQAGNRTPTDFFALSNITGPFGQIAGTGN
                     TTIDRTFLVNLPAQVGGLPAVPSIGWTFGKLGGDFALDMRLSAGELLGLSKVIAAPKI
                     TTLDKREAKISQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEEGKRILMR
                     VRATRNAVGARSNPAGPSIDRREATTQVIVKDGETMVIGGVFVDTQSNNVQGVPYLSR
                     MPVLGWLFKNKSETVSKQELLIFLTPS"
     gene            complement(1952..>2159)
                     /locus_tag="VN039_05095"
     CDS             complement(1952..>2159)
                     /locus_tag="VN039_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HWV45398.1"
                     /translation="TYGYTAMVQTPEGHGYTVRRGTRIGQNNGVVSAITERGIIVQER
                     FTDVYGKKQEREHVKLLHPKEGSE"
BASE COUNT          471 a          632 c          519 g          537 t
ORIGIN      
        1 atgctggggg tgaggaaaat gagcaattcc tgcttagaca cggtctcgga tttgttcttg
       61 aacaaccacc caagaaccgg catgcgtgaa aggtagggaa caccttgcac gttgttgctt
      121 tgcgtatcga cgaagacacc accaatcacc atggtttcac catctttgac gatcacctgc
      181 gtggtggctt ctcgtcgatc gatactcggc ccagccggat tgctccgtgc gccgacggca
      241 ttccgagttg ctcggacgcg catcagaatt ctcttcccct cttccttcgg atcacgcgag
      301 gtgatttgcg gcgttacgtt caattccaag ttcgcatcga caaatgtcgt ttgcgtaccc
      361 tgtaaggatg tcgtctggaa gggaatcgat tcgccttggg aaatcttcgc ttcccgcttg
      421 tccaacgtgg tgatcttcgg cgctgcaatc actttgctca accccagcaa ctctccggcc
      481 gagagccgca tatccagagc gaaatcgccg ccaagcttcc caaaggtcca tccaatcgac
      541 ggaacagccg gcaacccacc gacctgagcc ggtaagttga ccaagaaggt tctatcgatc
      601 gtggtgttcc ctgttccggc gatctgtcca aacggacccg taatatttga gagggcaaag
      661 aaatcggtgg gtgtgcgatt gcccgcttgg aacccccact gaatccccaa gcctctcgca
      721 tacacggtgt ccgcttgaac gatgcgcgct tcaatctgaa cttgagggac ttccaggtcg
      781 agtccctcca cgagctgttt gacgattgcc aacttggatt ccgtctcgcg cacaatgagc
      841 gcattgcttc cctgattgaa ttgcatgaca ccgcgtgggc ttaaatattg ccgcagtgat
      901 gtcataacct cttgcgcctg gagattcctg atataaaaga cgcggtcgat taattcttcc
      961 gccttggtct tggagtcctt ggcccgtgct tcttcatcct gctgcttggc aatattcgcc
     1021 agagaatcca cccatacaat gctcccttgg cgaatcatgc caaggccgtt catcttcaaa
     1081 agcatatcca gcgcctgatc ccacggtaca ctcaccaatt tcatggtgac cttcgacttg
     1141 acgccttctc ccacgacgat gttgaagccg ctgacttcgg ctatcaatcg aagaatgttg
     1201 gtaatgtcgg cctgctgaaa atccagcgag atacgccggc cgacatatcg tgtttgccct
     1261 gccacaatgt catcggatct gtcggccttt tcaaccggaa cactatctcc cgccatctga
     1321 acgcgctgca ctttaaactg agactgcgtg ggaccgacca ttcgcatcat ccttttgccc
     1381 acacgatccc ccgactcgaa caacattttt cgtgccctgg gggtgaataa aacgcgctct
     1441 ctttccccat caaactgcgc cgcgaccacc gtgggtattt tgtgctcgct gggtttcagt
     1501 gtgatcaata ccttattttc ttcccgggca agggaaaaaa tcggcctttc cggcacatcg
     1561 aacaccagcc tgaccttatc agcatggtgt cccactctga ttttcttcag caggtagtgg
     1621 tcggcccgta tcactgatcg tctaagggcg gacgatacgg caggaatatc gacgattaag
     1681 cgggattcat ctaaaaaatt ggcctcagga aatagctgac catccccggc gaccaccaca
     1741 gtcaccagat ccgtttcggg acggacctca atggcggtca cggcctgcgc cagcaatcgt
     1801 gaggtggtgg cctcacctcg ctttgtcatg ccaatctctt cagcgaatgc aggtgtctcg
     1861 gcaaatatgg ccagagcggc gagcgacagc cccaaggctc cgatgaaaca tccggcagtt
     1921 agggaattat ctgcgcgtaa aaatagtggt ttcattctga gccctcttta ggatgtagaa
     1981 gcttgacatg ttctcgctcc tgcttcttgc catacacatc agtgaaccgt tcctgaacaa
     2041 tgatgccgcg ctcggtaatt gcgctgacta ctccattatt ttgcccaatc ctcgttcccc
     2101 gtcgaactgt gtatccatgt ccttctggag tctgcaccat agcggtatag ccgtatgtc
//
LOCUS       DATMTI010000305         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_774994, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000305 DATMTI010000000
VERSION     DATMTI010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774994"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VN039_05100"
     CDS             complement(<1..708)
                     /locus_tag="VN039_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV45399.1"
                     /translation="MSTSVLKVSDLTKRFGDFTAVNGVSFEIRPGEILGLLGPNGAGK
                     TTTIQMLLGLVTPTSGSIHMFGLDLSTHREEILQQVNFSSTYISMPQSLTVEENLWVV
                     ARLYGVSDIARRVNDIVKKLEMEEFRCKVTRKLSSGQLTRLTLAKAFLTEPRILFLDE
                     PTASLDPDIADKIRSLLKEERRSSGLSILYTSHNMREMEEMSDRIIFLQRGRIVAEGT
                     AQEIIDRFGKADLEEVFL"
     gene            complement(730..1440)
                     /locus_tag="VN039_05105"
     CDS             complement(730..1440)
                     /locus_tag="VN039_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWV45400.1"
                     /translation="MNWKSKAILLPALVIGAMALVGTALPFTDHPTFCAGCHTIAPAY
                     ESWAKSSHKEITCVACHVRPGVQGWLSDKVLAGARDAAIYVFGTPTDAHDLKAQVDSG
                     VCLSCHRHILRVSEIAPRDLPPPVKQVGLVMNHRQHMEAFKARGQDEGCTTCHSGVVH
                     GEPIKGYPTVIPRGHVSADSQPWYPPHPEGSYLRARALSDCFRCHDGKAQHRGKTLDR
                     KCETCHIPEKISGALLFN"
     gene            complement(1424..1942)
                     /gene="mobB"
                     /locus_tag="VN039_05110"
     CDS             complement(1424..1942)
                     /gene="mobB"
                     /locus_tag="VN039_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HWV45401.1"
                     /translation="MPIPIVSFIGRSNSGKTTLIERIIPELVKAGYRVATVKHAGHGF
                     DLDTEGKDSWRHKRAGAGSVVVVSKGSLALFADVSEQLKVEEVRDRFLDASYDLIIAE
                     GWKSEGYPKIIVVRDQLGEIAYSSDGLLAVVTDKPIDLPAPVLHLDDVAGVAALLIKH
                     FPLRRREHELEV"
     gene            complement(2029..3327)
                     /gene="glp"
                     /locus_tag="VN039_05115"
     CDS             complement(2029..3327)
                     /gene="glp"
                     /locus_tag="VN039_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWV45402.1"
                     /translation="MKAEDIMTGLTQLYDAQRVVLDAAPVLGFEKVSILEALGRVLSE
                     DVVAERDNPPWDNSAMDGFAVKWEDIKQEQAIQKPVTLSVIEDVPAGMMPSKTVGLGQ
                     AIRIMTGAPIPQGADTVLKVEDTEHTPDSVRVFKTEPKGANIRPQGEDVKKGDRIIAK
                     GTRIRPSEVGMLAILAKSFVCVYQRPRVAILATGDELADLDERFSEEKIINSNSYGMA
                     AAVQEAGGVPLLLGIARDTPMALKEKISQGLNADMVVLSGGVSMGDYDFTKVVFRELG
                     AEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPALLKMSGCRSYG
                     RPIIQAIFQERFSKRGDRRHFLRGILTRGEGVFKVRTTGDQGSGILTSMVKANCLIDV
                     PVEVERLNPGDEVVVQLLSGEAWPAHTEQGHSGTHRLSCC"
     gene            complement(3324..4295)
                     /locus_tag="VN039_05120"
     CDS             complement(3324..4295)
                     /locus_tag="VN039_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWV45403.1"
                     /translation="MARELNVINNGSGDGDACTYMWACAICDENERCQKDKEGHSRWL
                     VAKRMARIEHKVLIMSNKGGVGKSTCTTNIAVSLALKGWHVGICDMDIHGPNIPKMVG
                     AEGQKLKISTSGGIIPFQAYNLKIASMSFLLQNSDDPIIWRDAYKYEFINQLLGGVDW
                     QDLNFLLIDLPPGTGNESVTTIDLLGTVSGAVIITTPQEVALLDSRKSVTFCKDSEVP
                     IVGIVENMSGLECPHCHTHIDVFRKGGGEASALDMGVPFLGRIPLDPDVVTQSDAGEP
                     FALFNSDTPTAEAYHRIANQVEAFCKKGASLLKVGGTATGLLKGGSQ"
     gene            complement(4340..>4935)
                     /locus_tag="VN039_05125"
     CDS             complement(4340..>4935)
                     /locus_tag="VN039_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWV45404.1"
                     /translation="IWRFVVPGLHAQERRFVGPFVCVSTLAFYTGVGFSFFFVLPFAL
                     NFLISYGVNAGFVPQISIAQYVGFALWFLTVFGLIFEVPLTLTLMAKLGWVDAPFLIQ
                     YWKWALLGSFVIAAILTPTPDPFNQTLMAGPMFLLYWVGIFGAKLFGKKTSADSHHAG
                     IPTVAMAGVGAGGATSGMSMPKSSGDDYVSVPGGRHR"
BASE COUNT         1126 a         1463 c         1262 g         1084 t
ORIGIN      
        1 caagaatacc tcttccagat cggctttccc gaatcggtcg ataatttcct gcgccgttcc
       61 ttctgccacg atgcgtccgc gctgaagaaa aatgatgcgg tccgacattt cttccatttc
      121 gcgcatgtta tgtgatgtgt aaaggatact caggccggat gatcgccgtt cttctttcag
      181 caacgatctg attttgtcgg cgatatcagg gtccaaactc gccgtcggct cgtcaagaaa
      241 gaggatgcgc ggttcggtaa gaaatgcttt cgccaatgtg agcctcgtca gctgaccaga
      301 cgataatttt cgggtaacct tgcagcggaa ctcctccatc tccagctttt tgacgatatc
      361 atttacacgc cgagcgatat ccgaaacccc gtaaaggcga gccacgaccc ataaattttc
      421 ttctaccgtg agcgactggg gcatggagat gtaggtagac gaaaagttga cctgctgcag
      481 aatctcctca cgatgagttg agagatccag accgaacatg tgaatagagc cggacgtagg
      541 ggttacgagt cccaagagca tctgaatggt ggtcgtcttc ccggctccgt tcggccccaa
      601 gagtcccagg atttctccag gcctgatctc aaaggatacc ccattgaccg ccgtaaagtc
      661 gccgaaccgc ttggtcagat ccgacacttt cagtactgag gtagacatca cgctgacgga
      721 ttcggtcgat taattgaaca gcaaggcgcc gctgatcttc tccgggatat gacaggtctc
      781 gcatttccgg tctagcgttt tcccgcgatg ctgtgccttc ccatcgtggc agcggaaaca
      841 gtcactgagt gcccttgcgc gaaggtagga gccttccgga tgcggaggat accaaggttg
      901 gctgtcggcc gatacatgtc cgcgtggaat gacagtggga taccccttga tgggttcacc
      961 gtgcacgacg ccggaatgac aagtcgtgca cccttcatcc tgtccccgag ccttgaacgc
     1021 ctccatatgc tggcgatggt tcatgaccag ccccacctgt ttgactggtg gagggagatc
     1081 gcgtggggct atctcggaca cacgaaggat atggcgatgg caactgaggc aaacgccgga
     1141 atccacctgt gctttgagat catgcgcatc ggtcggcgtt ccaaatacgt agatagctgc
     1201 atctctggct cccgccagga ccttgtcgga taaccagcct tgaacaccag gccgtacgtg
     1261 acacgcgaca caagtgattt ccttatggga agatttggcc cagctctcat aagcaggggc
     1321 gatcgtgtga catcctgcgc agaatgtcgg gtgatcggta aacgggagcg ccgttccaac
     1381 cagagccata gctccgatca ccagggccgg taacagaatg gccttagact tccagttcat
     1441 gctcccggcg tctaagggga aagtgcttga tcaaaagggc ggcgacgccg gccacatcgt
     1501 ccaggtgcag cacaggcgca ggaagatcga tcggcttgtc ggtgaccacc gccaggaggc
     1561 cgtcggaaga atacgcaatt tcaccgagct gatcacgaac gacgatgatc ttcggatacc
     1621 cttcgctttt ccacccttcg gcgatgatga ggtcgtagga ggcgtccagg aaacgatcgc
     1681 gcacttcctc caccttcagc tgttcggaga catcggcaaa caacgcgagg cttcccttcg
     1741 agaccaccac aacactgccg gcaccggcac gtttatggcg ccaactatcc ttcccttccg
     1801 tatcaagatc aaacccgtga ccggcatgtt tgaccgtcgc cacgcggtat ccggccttga
     1861 ccaactcggg gatgatgcgt tcaatcaagg ttgttttacc gctgttcgac cggccgataa
     1921 acgaaacgat gggaatcggc atagaaatca tcagtcactg atcatcgatc accggacaaa
     1981 agagaatcct tctctcatga ccactgacga ttgaccgttg actcctttct agcaacaaga
     2041 gaggcggtgt gtaccggaat gcccttgctc ggtatgagcg ggccatgctt caccgctcaa
     2101 taattgaacc accacctcat caccgggatt gagccgctca acttctaccg gcacatcgat
     2161 gaggcaattc gccttgacca tggacgtgag gataccagag ccctggtctc cagtcgttcg
     2221 aaccttgaac acgccttctc ctctggtgag aatgccgcgc aggaaatgcc gccggtcgcc
     2281 ccgcttggaa aatcgttcct ggaaaatagc ctgaattatc ggacgtccat agctccgaca
     2341 cccgctcatt tttagtaacg caggtcgcac caattgctcg aaggttacca tcgacgaaac
     2401 cggattgccc gggagcccga acgcgagctt gccctggatc ttcccgaagg ccagcggctg
     2461 accgggtcgg atggcgagtt tccaaaaatt catctcggcg ccaagttccc ggaacaccac
     2521 tttggtaaaa tcatagtcgc ccatcgagac tccgccggac aacaccacca tgtcggcatt
     2581 caacccctgg gatatctttt ctttgagcgc catcggcgta tcacgcgcga taccgagcaa
     2641 tagcggaacg cctcccgctt cctgcacggc agcggccatc ccatagctgt tcgaattgat
     2701 gatcttctcc tcgctgaacc gctcgtccaa gtcagccaac tcgtcgccgg tcgcaaggat
     2761 cgccacccga ggccgttgat agacgcacac gaacgacttc gccaggatcg ccaacatccc
     2821 aacctcgctg ggacggattc tggtcccttt tgcgataatg cggtcccctt ttttgacgtc
     2881 ctcaccctga ggtctgatat tggctccttt gggttccgtc ttaaacacgc ggaccgaatc
     2941 aggtgtatgt tccgtatctt caaccttcag aactgtatcc gccccttgag ggatcggagc
     3001 cccggtcatg atccggatgg cctgacccaa accgaccgtt tttgacggca tcatccctgc
     3061 cggaacatcc tcaatgaccg agagggtgac cggtttctgg atggcttgct cctgtttgat
     3121 atcctcccat ttcaccgcaa acccgtccat ggctgaatta tcccatggtg gattgtcgcg
     3181 ctcagcgaca acgtcttcac tcagcacacg gcccaaggcc tccagaatcg agactttttc
     3241 aaaacccaat accggcgcag catcgagtac aacccgttgt gcatcgtaga gttgggtcaa
     3301 tccggtcatg atatcctcgg ctttcactgg ctgcctccct tcagtagtcc agtggccgtc
     3361 cccccgactt tcagcaacga tgcgcctttt ttacagaagg cttccacctg attggcgata
     3421 cggtggtacg cttccgccgt cggcgtatct gagttgaaca gcgcaaaggg ttcacccgcg
     3481 tcggattgtg tgaccacgtc cgggtcgagc ggaatccgcc ccaaaaatgg gactcccatg
     3541 tcgagcgccg acgcttcacc accgcccttc cggaagacat caatatgagt atggcaatgg
     3601 gggcattcga gcccgctcat attttccacg atgccgacga tcggcacctc gctatctttg
     3661 caaaacgtga ccgattttcg ggaatcgagc agcgccactt cttgtggcgt ggtaataatc
     3721 acggcaccgc tcaccgtgcc gagcaaatcg atcgtcgtga ccgactcatt cccggttccc
     3781 ggcgggagat cgatcaggag aaaattcaaa tcctgccaat cgacaccccc aagcaactga
     3841 ttgatgaatt cgtacttgta ggcatcgcgc cagataatgg ggtcatccga gttctgcagc
     3901 aaaaacgaca tcgaggcgat cttcagattg taggcttgga agggaataat cccgccggag
     3961 gtgctgatct tgagcttctg accttccgca ccgaccattt tgggaatgtt ggggccgtga
     4021 atatccatgt cacagatccc gacatgccat cctttaagcg caaggctcac cgcgatgttg
     4081 gtcgtgcacg tgctctttcc cacaccaccc ttattgctca tgatgaggac tttgtgctcg
     4141 atgcgcgcca tacgcttggc gaccagccat cggctatgcc cttctttatc cttttggcac
     4201 cgttcgtttt cgtcgcagat ggcacaagcc cacatatagg tgcaggcatc tccatctccg
     4261 cttccgttat tgatgacgtt gagttcacgt gccatggaat gtcttttctg cgaccattgc
     4321 tgtcgatcgc cctccctcgt tagcggtgtc gtcctccggg aacgctgaca tagtcatcac
     4381 cggaagattt cggcatggac atgcccgagg tggccccccc agctcctacc ccggccatgg
     4441 cgactgtcgg gatacctgca tgatgactgt cagccgatgt tttcttaccg aacaacttcg
     4501 cgccgaagat gccgacccag tagagaagaa acatggggcc ggccatcagc gtctgattaa
     4561 acggatcagg cgtgggggtc agaatcgccg cgataacaaa cgatcccaac aacgcccact
     4621 tccaatactg aatcagaaac ggcgcatcga cccaacccaa ctttgccatg agtgtgaggg
     4681 tcaacggcac ttcaaagatc agcccaaaga ccgtcaagaa ccacagggca aaaccgacgt
     4741 actgggcgat cgaaatctgc ggcacgaatc cggcgttcac cccgtacgaa atcaaaaaat
     4801 tcagggcaaa tggaagcacg aagaaaaatg agaatccgac accggtgtag aatgccaacg
     4861 tgctcacaca gacgaacggc ccgacaaacc gtcgctcttg agcgtgaagt cccggaacga
     4921 cgaacctcca gatct
//
LOCUS       DATMTI010000306         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_562922, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000306 DATMTI010000000
VERSION     DATMTI010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562922"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VN039_05130"
     CDS             complement(<1..1003)
                     /locus_tag="VN039_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV45405.1"
                     /translation="MKKTIAFTSAAFNYVPKVRTLCNSMRKHHPEFEIVLALADKVPA
                     GTSWESEPIDRVLPVGDLEIPDLERWTFFHAIVELATAIKPFALLKLLEDPSVERVIY
                     FDPDMMLFSRLDDLLDDVQGASIGLTPHQTKPETTRRRVMDNEISSLKHGVYNLGFLV
                     VNNNAEGRRFARWWADRIYDFCVADIPNGLFTDQRWVDLAPAFFDDVKILKSPRFNVA
                     PWNVSTRELAGDTENGFTVDGEPLGFYHFTGFDSGAHAIMAEVNAPGNDSLRQLIEFY
                     RHETRLDRNDIISNTPWAFGTYDNGLKIDTLHRRIYRSRDDLREVFPSPFSTEGESTY
                     "
     gene            complement(1017..>1709)
                     /locus_tag="VN039_05135"
     CDS             complement(1017..>1709)
                     /locus_tag="VN039_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45406.1"
                     /translation="KSLSIPFLPYLSGVSIPDWRTEWEHLLRHCSIHAFSASTVEIFR
                     KAFPHLHSKVIPVEPHSMEYFKGRPIDYGRNVSRYRVGIVGHINGPKGAAMVESAARI
                     IEQRSLPIELVVFGSIDATGHYESIIQAGGFNADNLADKLEEYDINVSWVPSVCPETY
                     SYVTSELIRLDIPLVCFDVGAPAERVSQYPLGHVVPDFDIAAVVDYFVRLREASLAAA
                     RPSILSHIDEAI"
BASE COUNT          438 a          443 c          444 g          384 t
ORIGIN      
        1 gatacgtact ttcgccttca gtcgaaaatg gtgaagggaa cacctcgcgc aggtcatcac
       61 gcgaacgata gattcgtctg tgcagggtgt ctatcttgag gccattatcg taggtaccga
      121 atgcccacgg cgtattggag ataatgtcgt tccgatccag cctggtttcg tggcgataga
      181 actcaatgag ctgtcgcaac gaatcattcc caggtgcatt gacctcagcc atgatggcgt
      241 gagcgccgct gtcaaatcca gtgaagtgat agaagcctag tggctcaccg tcgaccgtga
      301 acccattttc ggtatctccg gccagttcac gcgtggatac gttccacgga gccacgttga
      361 atctcgggct cttgagaatc ttcacatcat cgaagaacgc cggagccaag tcgacccatc
      421 gctggtcggt aaacagcccg ttaggaatat cggccacgca gaagtcgtaa attctatctg
      481 cccaccaacg tgcgaaacgg cgcccctctg cattgttatt gacaaccagg aaacccaggt
      541 tgtaaacgcc atgtttcaaa ctgcttatct cattatccat cacccgccta cgcgttgttt
      601 caggcttggt ttggtgaggc gtcaagccaa tgctggcgcc ctgtacgtca tcaagcaggt
      661 cgtccagcct ggaaaagagc atcatgtctg gatcaaaata gatcacgcgc tcaacggaag
      721 gatcttcgag caatttgagc agagcaaagg gcttgatcgc ggttgcaagt tccacgattg
      781 cgtggaaaaa agtccaacgt tctaggtccg ggatttcgag gtcgccaacc ggcaacaccc
      841 gatcaattgg ctcagactcc cacgacgtac ccgcgggaac cttgtctgct agcgccagca
      901 caatctcgaa ttcagggtga tgtttgcgca tcgaattgca gagcgtacgg accttgggca
      961 cgtagttgaa agcggcggat gtgaatgcaa tagttttttt cattgaagca ggccttttag
     1021 atagcttcat ctatgtgact aaggatcgat ggccgggcag cggccaagga agcctcccgc
     1081 aaccgaacga agtagtcaac aacagcagct atatcgaaat ccgggacaac atgaccaaga
     1141 gggtattgtg agacgcgttc cgcaggcgcc ccgacgtcga agcaaaccaa tggaatatcg
     1201 agacgaatta attcggaagt gacataagaa taggtttcag ggcagacgga aggcacccaa
     1261 gatacattta tatcgtattc ctcgagcttg tctgccaagt tatcggcatt aaatcctccg
     1321 gcctggatga tcgactcata atgcccggtc gcatcgatag agccaaatac aaccaactcg
     1381 atcggaagcg agcgctgctc aataatacgt gcggcggact cgaccatcgc agcacccttt
     1441 gggccgttga tatggcctac gattcctact ctgtatcgcg aaacattgcg gccatagtct
     1501 ataggcctac ccttgaagta ctccatgcta tgtggctcga cagggatgac cttgctgtga
     1561 aggtgcggaa acgcttttct gaatatctct acggttgaag cggagaaggc atgtatcgag
     1621 cagtgcctca gcagatgctc ccactccgta cgccagtccg gaatggatac ccccgaaaga
     1681 tatggaagaa atggaatact tagggactt
//
LOCUS       DATMTI010000307         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_520952, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000307 DATMTI010000000
VERSION     DATMTI010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520952"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VN039_05140"
     CDS             <1..651
                     /locus_tag="VN039_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45407.1"
                     /translation="DNSRVNFANPSSDYGRSNYDQPLNNTTAVIYDLPYGHGRHFGGS
                     SPYVLNALLGGWQVTLINSMNSGLPTNLNYSNSTSTGTNVTDLYTYRPNVIGNPVAPS
                     SNRKKTNTALTGYLLKSTGPGVAGAYVPTTGNPFGNAQRNMVVSPAFYQADLGLHKAF
                     PLWREGSTFDFRAEAFNVLNKVNYQAPNGNVSSSSYGSITSAFPARQLQLAAKIIF"
     gene            766..>1556
                     /locus_tag="VN039_05145"
     CDS             766..>1556
                     /locus_tag="VN039_05145"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HWV45408.1"
                     /translation="MIKKSNSDTGQMMNIRKVTQISLALAVFATTVSALAQEPTAAEQ
                     DQRVHVNQIQVIGSHNSYHSGFPPSARKLMEKLSPQGLRSLDYHHAPLADQLSGGVRQ
                     IEIDVYADTKGGRYAHPAILKMVAKAGLPADPVFDPQHVMDKPGFKVLHMQDIDVRST
                     CMTLIACLTDVRGWSKQHPQHLPIFILIETKEGKDRNVPNAVVPEPFTAPVFDALDKE
                     IRSVFTPDEMITPDDVRGSSATLLEAVHAGKWPTLAQARGKVIFLL"
BASE COUNT          341 a          448 c          437 g          330 t
ORIGIN      
        1 gataactccc gtgtgaactt cgccaatccc agcagcgact acggtcggtc aaactatgac
       61 cagccgctga acaacaccac cgcagtcatt tacgatctac cgtatggaca cggccgtcac
      121 tttggcggta gctctcccta cgttctgaac gcgctcctcg gcggctggca ggtgactctg
      181 atcaactcca tgaacagcgg tctgccgacc aacctcaact actccaacag cacgtcgacc
      241 ggaacgaatg tgacggacct gtatacatat cggccgaatg ttattggaaa tccggtggct
      301 ccttcgtcga accgcaagaa gacgaatacc gctcttacgg gctacctgtt gaagtcgact
      361 ggccccggag ttgccggagc ttacgtaccc actacgggta acccgtttgg aaatgcgcag
      421 cgaaacatgg tcgtttcgcc tgccttctat caggcggacc tcggattgca caaagcgttt
      481 ccgctatgga gagagggttc gaccttcgac ttccgcgcgg aggccttcaa tgtgctcaac
      541 aaggtcaact atcaggcgcc gaacggaaat gtcagcagct cctcctacgg ctccatcacc
      601 tccgcctttc cggcacgtca gctgcagcta gccgctaaga tcatctttta gctgcgcaca
      661 agacgcgtcg gatttacatg agtacggcaa ggacacggcg ctgagtcttc tcgatcagcg
      721 ccgtgtcctt gtttcaatcg atctgttcga caggccggag gcccgttgat caaaaaatca
      781 aactcagaca cgggacaaat gatgaacatc cgcaaggtga ctcaaatctc tctggccctg
      841 gcagtattcg ccacgaccgt atcggcgctg gcgcaagagc cgaccgctgc tgaacaggac
      901 cagcgggtcc atgtaaacca gatccaggtg atcggttcgc acaacagcta ccattcaggg
      961 tttccgccga gtgcgcggaa gctgatggag aagctgagcc ctcagggatt gcgttccctc
     1021 gactatcatc acgctccgct ggccgatcag ttgtcgggcg gcgtacggca gatcgagatt
     1081 gacgtttatg cggacacgaa gggtggaagg tatgcgcatc ccgcgattct gaagatggtg
     1141 gcgaaggctg gcttgcctgc cgatccagtg ttcgatccgc agcatgtgat ggacaagccc
     1201 ggattcaagg tgctgcacat gcaggatatc gatgtgcgca gcacctgcat gacgctgatc
     1261 gcgtgtctca ccgatgttcg tggctggtcg aagcagcacc cgcagcatct gccgatcttt
     1321 atcttgatcg agacgaagga agggaaggac cgcaacgttc cgaatgcagt ggttcccgag
     1381 ccatttaccg ctccggtgtt tgacgcgctc gacaaggaga ttcggtcggt cttcacgccg
     1441 gacgagatga ttacaccgga tgacgtgcgc ggcagttcgg cgacgttgtt ggaggctgtc
     1501 catgctggca aatggcctac tttggcgcag gcgcggggca aggtaatttt tctgct
//
LOCUS       DATMTI010000308         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_605502, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000308 DATMTI010000000
VERSION     DATMTI010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605502"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1303
                     /locus_tag="VN039_05150"
     CDS             119..1303
                     /locus_tag="VN039_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV45409.1"
                     /translation="MTHDSLRQSLCEHLAWWGFKHFSSDRDYFAWQRQRLSSEDLKRL
                     AFHAEQKRRGDRHDEIMFYDLAAHPKIRPVLYSQRYEYYEEIGLRTTAHLGQAKDVLD
                     FGCGIGVLTIFYARLFPEKNFVGVDRSSASIAVARERTDKLGLRNLRFECVDVEVEPL
                     SGSYDLVVATHALLQAEQDLGLPSRNWRTFERGHNLSQQRSFEERTGLAVRLDGLCGV
                     MNSSGRIIVSEKTRQLARRVPLQRALANRGLQLAERPIMIRYRTVEEVVDDGPFYVLD
                     RGGEATLNWDEEPEQDQGPLFNPDAVSMSAGDVDVPLYENHWPSAQAAWEGLKDRQVI
                     RETTRQASDGRQVHAERGSSRGRHYLYCANTFDQRQLVIVEEARSAMLDEYYQEIIRE
                     LP"
     gene            1408..>1645
                     /locus_tag="VN039_05155"
     CDS             1408..>1645
                     /locus_tag="VN039_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45410.1"
                     /translation="MKETNVLSVSRLAGYQPPEGRQDVQRGIELAKADPRLAGKVEQL
                     QGHGLLMQPDRGFFRNDPGYAHRTIWITFSQGQDG"
BASE COUNT          363 a          436 c          481 g          365 t
ORIGIN      
        1 cccgctgaat tttttccctg catcgttcgt ttgccccact caagatacgc ttctgtgcgt
       61 ctcgtacgtc aggctttctg cggccttgcc gggaaccgtt ttgatccggg tatgagatat
      121 gactcatgat tcgctgcggc aatcgctttg cgagcatctt gcgtggtggg ggttcaaaca
      181 cttctcatcc gaccgtgatt atttcgcgtg gcagcgacag cgactgtcat cggaagatct
      241 gaagcggctc gcgtttcatg ccgaacaaaa acgccgaggc gatcgtcacg atgaaatcat
      301 gttctatgat ttagccgcgc atccgaagat ccgccctgtt ctctatagtc aacgctatga
      361 atattacgag gagatcggtt tgcggacgac ggcccacctc ggccaagcga aagacgtcct
      421 tgattttggc tgtggcatcg gagtgctgac gatcttctat gcacgcctct tcccggagaa
      481 gaattttgtg ggcgtcgatc ggtcatccgc ttccattgcc gtcgcacggg aaaggaccga
      541 caagcttggg ttgcgcaacc tccgctttga gtgtgtggat gtggaagttg agccgctctc
      601 aggttcatac gatcttgtgg tggcgaccca tgcgctgctg caggcggagc aagatctcgg
      661 tcttccgagc cggaattggc gaaccttcga acgaggacac aatctctcgc agcagcgatc
      721 attcgaagag cggacagggc ttgcggttcg attggacggg ctgtgcgggg tcatgaactc
      781 atccggccgc atcattgtga gtgagaagac gcggcagttg gccagacgag tgccgcttca
      841 gcgcgcgttg gcgaacagag gactgcagct cgccgagcgc ccaatcatga tccgatatcg
      901 aacggtggaa gaagtggtgg atgacggccc gttttatgtg ctggatcggg gaggcgaggc
      961 gactctgaat tgggatgagg agccggaaca agatcaggga ccgctcttca accctgacgc
     1021 cgtttcgatg tcggcgggtg atgtcgatgt gccgctctac gagaatcatt ggccttcggc
     1081 acaagcggct tgggagggat tgaaagatcg acaggtcatc agagagacca cccgtcaagc
     1141 atccgatggc cgacaagtac atgccgagcg cgggagctct cgcggacggc actatcttta
     1201 ctgcgccaac accttcgacc agcggcaact ggtcatcgtc gaagaagccc gttcggcgat
     1261 gctcgatgaa tactatcaag aaatcatacg tgagctcccc taggaaagtt actggctaag
     1321 ctctcgtttg gttgttgtcg tcacacaaca aacctgacac ggcttgtgta ttacagttac
     1381 agccaaaata ttgccgtgga agtccatatg aaggaaacga atgtgctcag cgtctctcgc
     1441 cttgcaggct accaaccgcc agaaggccga caggatgtac aacggggaat cgagttggcg
     1501 aaagccgacc ctcgtctcgc aggcaaggtt gaacaactcc agggccacgg attactgatg
     1561 caacccgacc gggggttttt caggaacgat cccggctatg cccaccgcac gatttggatc
     1621 acattttccc aaggacagga tggag
//
LOCUS       DATMTI010000309         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_351281, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000309 DATMTI010000000
VERSION     DATMTI010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351281"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VN039_05160"
     CDS             <1..1088
                     /locus_tag="VN039_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV45411.1"
                     /translation="AARESFKAHEPMTGTSLFTILPLDPVVQKRLQAEFHITGDSALA
                     TDTISLEAVPRDPLAPSVDHESTEPRAELHIASRIYQYQWFRLPTRPGEEDSIGLVLR
                     DTTDESRLQDQLVQAEKTGSLGVLTAGIGHELNNPLFGIIGLGEVIQDEQDLEHIKNC
                     ARDIVAHGRRMATIIRDFTGVTNREAFDKQVSVYLEQILDQVLATAQTTVEMKDIAIH
                     KIYAGNTSVMVFPDQLRQALVNLVTNAVQAMQGTGTLTLTTRVSDHTVIVTIADTGPG
                     ISKQHLTKVFDPFFTTKGQGEGSGLGLTVARRIIRKFGGDLRIESAEGSGTACIVTLP
                     TNPPLSPEGGPWTADAAHSEPQPSHSS"
     gene            1037..1630
                     /locus_tag="VN039_05165"
     CDS             1037..1630
                     /locus_tag="VN039_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HWV45412.1"
                     /translation="MDSRRCAFRTATIAFFLVGLLIWIPFPSAKENPGLVGIAPEKVA
                     DYIYAILEADRSIYTTQIVNRMQERGIVWAAEHWEQNNALPLPAQFLQHSGRLVAESG
                     RGIRYRLIGLFPIYQRNAPATEFERKALIQLQRQPDRAITGIVSSGKKQYFQAIYSDR
                     ALSSACITCHNSHPLSPKQDFKLNDVMGGIAITIPLE"
     gene            complement(1705..>2319)
                     /locus_tag="VN039_05170"
     CDS             complement(1705..>2319)
                     /locus_tag="VN039_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWV45413.1"
                     /translation="FALVTNFFIMLLVLFFLFTDGRQWLAVLYDLIPMEESHKSKILT
                     RLDQTIRAVVKGMLVTAIVQGLLAGMAYMALDVPFPMGLTALTIVLAPIPFGGTGLVW
                     GPVVFYLFWMGATGKALAMLVWGVGVVSMIDQFLRPWLIGQDIQIPVLLLVLSVLGGL
                     ALYGLLGLFVGPILISLLITAVQIYRDEYHLRHTVVVMSSSTPP"
BASE COUNT          587 a          684 c          597 g          451 t
ORIGIN      
        1 aggcggcccg cgaatccttc aaggcccatg aaccgatgac gggaacgtca ctgttcacga
       61 ttcttccgct cgatccagtc gttcagaaac ggcttcaagc ggaatttcac atcactgggg
      121 attcagctct cgccactgac acgatctccc ttgaggcggt gccgagagat ccactggcac
      181 cgagcgtgga tcatgaatca accgagcctc gcgctgaact ccacatcgcc tcgcgcatct
      241 atcaatacca atggttccga ttacccacga gaccaggaga agaggacagt atcggactgg
      301 tgcttcgaga taccacggac gagagccggc tacaagacca gctggttcag gcggaaaaga
      361 cgggaagtct tggtgtgttg accgccggca tcggccacga actcaacaac cctttgttcg
      421 ggatcatcgg gttgggggaa gttattcaag atgaacagga cttggagcat atcaagaatt
      481 gcgcccgaga catcgtggct catggacgtc gcatggcgac gatcattcga gattttaccg
      541 gcgtgaccaa ccgtgaggcc ttcgacaagc aagtgtcggt gtatttggag cagatattgg
      601 accaagtgct ggccacggca cagaccaccg ttgaaatgaa agacatcgcg attcacaaaa
      661 tctatgcggg caatacgtcc gtgatggtat tcccggacca actccggcaa gcgttggtga
      721 atcttgtgac caatgccgtc caagccatgc aaggtaccgg cacactcacg ctgacgacgc
      781 gcgtgtccga ccataccgtg atcgtgacga ttgctgatac cggtccgggc atctcaaaac
      841 aacacttaac caaggtattc gacccgtttt ttacgacgaa ggggcaaggg gaaggttccg
      901 gccttggact cacggtcgca aggcggatta tccgaaagtt cggaggcgat ctccgcatcg
      961 agagcgccga gggcagcggt accgcttgca tcgtcacgct cccgaccaat cctcccctgt
     1021 ctcctgaagg aggtccatgg acagccgacg ctgcgcattc cgaaccgcaa ccatcgcatt
     1081 cttcttagtt ggacttttga tctggattcc gtttccatcc gccaaggaaa acccggggct
     1141 cgtcggtatt gccccagaga aggtcgccga ttatatctat gccatcctcg aagccgatcg
     1201 ctcgatctat accacacaga tcgtcaatcg catgcaggag aggggcattg tctgggcggc
     1261 ggaacattgg gagcagaata acgccctccc ccttcccgcc caatttcttc agcattccgg
     1321 acggttggta gcagagagtg gacgaggcat ccgctacagg ctcatcgggc tcttcccgat
     1381 ctatcagcgc aacgcccccg ccaccgaatt cgaacgaaaa gccctgatcc aactccaacg
     1441 gcagccggat cgggcgatta ccggaattgt gtcgagcggc aagaagcagt actttcaagc
     1501 catctattca gaccgggccc tctcttccgc ctgcatcacc tgccacaaca gccatccgtt
     1561 gagccctaaa caggacttta agttgaacga tgtgatgggt ggaatcgcca ttacgattcc
     1621 actagagtaa cgacggatcc gtcattcgtc caacggagaa tcaagaaggc ggtccaggat
     1681 tcgcattcgg cgcagaccca agctttacgg tggtgtggaa gagctcatca ccaccaccgt
     1741 gtgccggaga tgatattcat cccgataaat ttgtacggcg gttatcagca agctgatgag
     1801 gatgggcccg acaaacagac cgagtaatcc atagagcgcc aacccaccca agacactcag
     1861 cacgagcagc aacacgggaa tctgtatatc ttgaccgatc aaccaaggtc gaagaaactg
     1921 atcgatcatc gagacgacgc cgactcccca caccagcatg gcgagcgctt ttccggtcgc
     1981 ccccatccag aacagataga agactaccgg cccccatacc aaccccgttc cgccgaatgg
     2041 aatcggcgcc aacacaatcg tcaacgccgt gagtcccatc ggaaacggca catcgagcgc
     2101 catgtaggcc atccctgcca ggagaccttg gacgatcgcg gtcaccagca tccctttcac
     2161 taccgcccga atggtttgat ctaaccgagt caagattttt gacttgtgtg attcctccat
     2221 cggaatcaaa tcgtataaga cagcgagcca ctgccggcca tccgtgaaga gaaaaaacag
     2281 caccaacaac ataatgaaga aattcgtcac gagcgcgaa
//
LOCUS       DATMTI010000310         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_732916, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000310 DATMTI010000000
VERSION     DATMTI010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732916"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VN039_05175"
     CDS             <1..771
                     /locus_tag="VN039_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWV45414.1"
                     /translation="GSEDQTGRQSTEATVHALFAPPSPETIPGDLRGEQIRLGYKMIV
                     DTQEYGKRYVGNALNCTNCHLDAGLNPNSASFVGVSTLSPEYRERAGRQVTLADRVNE
                     CFERSMNGKSLPSDSVKLTAIVAYIEWLSQNMPPGSTVPWRGIPPLTSAHQADPLHGK
                     TVFEKKCAFCHGSDGQGTMAAPPLWGPRSYNIGAEMARVSVAAAFIKANMPRGWGWTV
                     TDDEAIDVAAYINVQPRPDFPDKIHDWPKGNKPADVPY"
     gene            1113..1556
                     /locus_tag="VN039_05180"
     CDS             1113..1556
                     /locus_tag="VN039_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV45415.1"
                     /translation="MTTLAEPGVPMGGFKTVGQIRATNALVFRRDQNAMGVAVELLAT
                     HTPGAPVVDERGTFIGFISEFDILRALEAKKDLNRLTAQDVMAKEHIAVTEETSFDEA
                     VRIMEDKRLLNLPVKKNGKVAYSITRHDLLRAWIGLGVSIEDAAG"
     gene            1608..2192
                     /locus_tag="VN039_05185"
     CDS             1608..2192
                     /locus_tag="VN039_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HWV45416.1"
                     /translation="MSSGKRIAGGMSIALCLLASWGTGNAGKESANSVPLETVADYIY
                     SVLAADRTFYTVHVVERMQRKGMIESSENWRLESALPLPAQFFQESASLAELTGSKVR
                     YRLISLNPINKQSSPRTEFEQKALEAVMAHPEQPYKGFVREGGGRYFQAVYADPAVSP
                     SCVTCHNADPRGAKHDYKLNDVLGGVLISIPASE"
     gene            2428..2907
                     /locus_tag="VN039_05190"
     CDS             2428..2907
                     /locus_tag="VN039_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWV45417.1"
                     /translation="MSEQKTVTAFYRKDHDRLDDLFKAFQTSKRSDFAKAKEAFKEFK
                     VGLQRHIVWEEELLFPMWEEKTGMIEDGPTPMMRFEHGQIKQLLDAIHQKVEGQNLDT
                     DQDEQALVNLLSSHNRKEERALYPAIDNVTSTEECAAVFSNMNSIPEDRYNSCCSQH"
     gene            2918..>3224
                     /locus_tag="VN039_05195"
     CDS             2918..>3224
                     /locus_tag="VN039_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45418.1"
                     /translation="MKDKTEVERRPVVCSPILSLNLSLLVSFSLLFASGLVTEVVSPS
                     DYAWAESPTSQAAAQLKGNIMRGREIFNGKGVCYYCHGIDGYIGRPPRLAADTAALIA
                     "
BASE COUNT          773 a          820 c          943 g          688 t
ORIGIN      
        1 gggagtgagg accagaccgg tcgtcagagc acggaagcga cagttcacgc attgtttgcc
       61 ccgccttcgc ctgaaacgat tcccggtgat ctgagaggcg agcagattcg actgggctac
      121 aagatgattg tcgacacaca agaatatggg aaacggtacg ttgggaatgc cctcaactgc
      181 accaattgtc atttggatgc gggactcaac ccgaattctg catcattcgt cggcgtcagt
      241 acactctctc cggagtatcg tgaacgagcc ggccgacagg tgacgctggc cgatcgagtc
      301 aacgagtgtt tcgaacgcag tatgaacggg aagtccctgc cgtccgatag cgtgaagctc
      361 accgccattg tcgcgtacat cgaatggtta tcgcagaata tgccgcccgg cagtacggtc
      421 ccgtggcgag gaatcccgcc cctgacctcg gcccatcaag ctgaccctct ccacggcaaa
      481 acggtctttg agaaaaagtg cgccttctgt cacggctccg acgggcaagg caccatggcg
      541 gcgccgcccc tgtgggggcc gcgttcctac aacatcggtg cagaaatggc gcgggtcagc
      601 gtggccgccg catttatcaa ggccaacatg ccgcgaggct ggggatggac ggtgacggat
      661 gatgaagcca tcgatgtcgc agcctatatc aatgttcagc ctcgaccgga ctttcccgac
      721 aagattcatg attggccgaa ggggaacaag ccggcggatg taccctattg agtagggcaa
      781 aacggtttac cacggcagag tgcacctgtt tcgcctgtca tggggtccga ctcttttcac
      841 cttgcttttg tcgcattttt ccccacctga ccatattgct ctgagtattc ttgcatcaca
      901 tcggccgccc actgcttggt ctagttgcct caaggcgtgg tttcactcaa tacctgtcgc
      961 acatgtcttg caatgtgacg tcatcagaat cgcactcaac tttctgaatc caatcttgag
     1021 aaggaaggct ctcagtcaat gaaggacatg ggcgagagat ttgtgcaaat aactgattca
     1081 gctagatcac ccatcaacca ggaggaattt tgatgactac gctagccgag ccgggggttc
     1141 ccatgggagg gttcaagacc gtcgggcaga tacgtgcgac gaacgcgctt gtttttcgtc
     1201 gtgatcagaa tgcgatggga gttgccgttg aactcctggc cactcatact cccggtgcac
     1261 cggtcgtcga tgaacgaggt accttcatcg gcttcatcag cgagttcgac attcttcggg
     1321 cgctggaagc caaaaaggat ctcaaccggt taacagccca agatgtgatg gctaaggagc
     1381 acatcgccgt gaccgaggag acgagttttg atgaagctgt gaggattatg gaagacaaac
     1441 gactcttaaa cttgccggtg aagaagaacg ggaaggtggc gtactcaatc actcgccatg
     1501 atcttttgcg agcttggatc gggctcggag tgtccatcga agatgcggcg ggctgaggac
     1561 gcgagcttcc gttgatccgg ccggggtact gtccaaggga gggtgtcatg tcatctggaa
     1621 agcggattgc aggaggaatg agcatcgcgc tgtgcttgct cgcgagttgg ggaacgggca
     1681 acgccggcaa ggaatcggcc aacagcgttc cattggagac tgttgcggac tatatctatt
     1741 cagtcctggc agccgacagg acattttata cagttcacgt tgtggagcga atgcagagga
     1801 aaggcatgat cgaatcgtcg gagaattggc gtttggaaag cgcgctccct cttccggctc
     1861 aattctttca ggagtcagca agcttggccg agctgacggg aagcaaagtg cgttatcggt
     1921 tgatcagcct caacccgatc aacaagcaga gcagcccgcg aaccgagttt gaacagaaag
     1981 cactcgaggc ggttatggcg catcccgaac aaccatataa aggcttcgtc agggagggag
     2041 gcggtcgata tttccaagcc gtctacgcag acccagccgt ttcgccaagc tgtgtgacct
     2101 gtcataacgc cgacccccgt ggcgcgaagc atgactataa gctcaacgac gtgctgggcg
     2161 gcgtcctgat ttcaatcccg gcatccgagt aatcggaggg tggtgggtgc ataggaacag
     2221 gccctaagca cccaccaggt tcaaaagcca tgcagctggc ctcaaacgca cagaggctcg
     2281 gttggaaagc cgattgtgag tgcccgttcc gggagatcgt cgtccttctt ggtgggagtc
     2341 ggctttgtgg tatggtagca gccggttgcg gaaagtccga cgacagatgc atgaggacag
     2401 ggtcgtattt ttattatgga gagaacaatg agcgagcaga agaccgtcac agcattttat
     2461 agaaaagatc acgacagact ggatgacctg ttcaaagcct ttcaaacgtc gaaacgctcg
     2521 gactttgcca aggccaagga agccttcaag gaattcaagg tcggcctcca gcggcatatc
     2581 gtgtgggaag aggagctgct ttttccgatg tgggaagaga agaccgggat gatcgaggac
     2641 gggccgacgc ccatgatgcg atttgaacat ggccagatca agcagttgct cgacgcgatt
     2701 caccaaaagg tggaagggca gaatctcgat accgatcagg acgagcaggc gctggtaaac
     2761 ctattgagct ctcataatag gaaggaagag cgggcgctct atcccgcaat cgataacgtg
     2821 accagtacgg aagaatgtgc agccgtcttc agcaacatga acagtatccc agaagaccgc
     2881 tacaatagct gttgtagtca gcactgaaag gaaatgaatg aaggacaaga ccgaggttga
     2941 gcgaagacct gtagtttgtt ctccgatcct tagcctcaac ctatcactgt tggtctcatt
     3001 cagtctgctc ttcgcgagcg gtcttgtaac ggaggttgtg tctccatccg attatgcctg
     3061 ggctgagtcc ccgacttcac aagcggctgc gcagttaaag ggtaatatca tgcgaggacg
     3121 ggagattttc aacggcaagg gtgtgtgcta ctactgtcat ggcatcgatg ggtatatcgg
     3181 gaggccgcca cgcctggcag cggatacagc cgcgcttatt gcaa
//
LOCUS       DATMTI010000311         1784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_11852, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000311 DATMTI010000000
VERSION     DATMTI010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11852"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VN039_05200"
     CDS             complement(<1..206)
                     /locus_tag="VN039_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45419.1"
                     /translation="MKVRAQVAMVMNLDKCIGCHTCSVTCKNVWTSREGMEYVWFNNV
                     ETKPGVGYPHEWENQERWNGGWER"
     gene            complement(203..>1784)
                     /locus_tag="VN039_05205"
     CDS             complement(203..>1784)
                     /locus_tag="VN039_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWV45420.1"
                     /translation="FLKHLLGAAHGILAPDLKEQEAPLPREVVWRDEAPTGKLDLLVT
                     LDFRMSSTCLYSDIVLPSATWYEKDDLNTSDMHPFIHPLCEAVNPLWESKADWDIFKA
                     IASKFSELAGPVLGTRKDLVLTPLLHDTPSELGQPTTADDWKRGDCEAIPGKTMPNMT
                     LVTRPYGKTYDRFIALGPLTRDLGTGAKGIVWDSKEEVEALGRINGETDNREPYPKLE
                     TARQASQTILTLAPETNGHVAVKAWEALSQRTGRSHKHLAEGRAEERFTFDDLVAQPR
                     KVISSPTWSGIESEKVSYTGSYTNVQELIPWRTLTGRQELYQDHPWMRLFGEQVCVYK
                     PPVNTRSVEAFRRHMKDGDENFLVVSWITPHQKWGIHTTYSETSPMLTLSRGGPIVWL
                     AEVEAREAGIRDNDWIEVVNANGALVARAILSQRIPRGVAMMYHAQDKIINMPLAPAT
                     GRRGGIHNSLTRIIMKPTHMIGGYAHLAYGYNYYGTVGTNRDEMVVVRKLDRVTWDAD
                     PVATQHPSDVSRSSMEVL"
BASE COUNT          341 a          541 c          504 g          398 t
ORIGIN      
        1 ttacgttccc aaccgccgtt ccaacgctcc tgattttccc attcatgggg atagccgaca
       61 cccggcttgg tttcgacatt gttgaaccag acgtattcca tgccttcacg cgacgtccac
      121 acgttcttgc aggtaacgga gcaggtgtga cagccgatgc atttatcaag gttcataacc
      181 atggccactt gcgcgcggac tttcataaga cctccatcga tgaacggctc acatcgctcg
      241 gatgctgagt cgcaacggga tcggcgtccc atgtgacacg gtcgagcttc cgcaccacga
      301 ccatctcgtc ccggttggtc ccgaccgttc catagtagtt atagccatag gccaaatgcg
      361 cgtacccacc gatcatatga gtgggcttca tgatgatcct tgtcaggctg ttgtgaatgc
      421 ctccgcgccg gcccgtggcc ggagccagcg gcatgttgat gatcttgtcc tgcgcgtggt
      481 acatcatcgc cactccgcgc ggtatccgct ggctgaggat tgcccgcgcc accaatgcgc
      541 cattggcatt gacgacctcg atccaatcgt tgtcccggat acccgcttct ctggcttcca
      601 cctccgccag ccaaacgatc ggaccacctc gggacagggt gagcatcgga ctcgtctcgc
      661 tgtaggtcgt gtggattccc cacttctggt gaggagtgat ccaactgacg acgagaaagt
      721 tctcatcgcc gtctttcatg tgacgacgaa acgcttcaac ggatcgagta ttcacgggcg
      781 gcttgtagac gcagacctgc tcgccgaaga ggcgcatcca gggatgatcc tgataaagtt
      841 cttggcgtcc cgtcagtgtc cgccagggga tcagctcctg gacgttggta tagctcccgg
      901 tgtaggagac cttttccgac tcgatgccgc tccaagtggg cgaggagatg accttcctgg
      961 gctgcgcaac caggtcatcg aatgtgaacc gctcctcggc ccgcccttcg gccagatgct
     1021 tgtgggatcg gccggtccgc tgggagaggg cctcccaggc cttgactgcc acgtgaccgt
     1081 tcgtctccgg cgccaacgtc aagatggttt gggatgcctg ccgagctgtt tccagcttcg
     1141 gatagggctc tcgattgtcc gtctcgccgt tgatccggcc caacgcttca acttcttctt
     1201 tactgtccca tacgattccc ttcgccccgg tcccgaggtc tcgcgtcagc ggcccgagcg
     1261 cgatgaatcg atcgtatgtt ttaccatagg gtcgcgtgac cagggtcatg ttcggcatcg
     1321 tcttgcctgg gatggcctca caatcgccgc gcttccagtc atcggccgtt gtcggctgac
     1381 cgagctcact cggcgtatcg tgcaacagag gcgtcaagac gagatctttc cgcgtgccaa
     1441 ggacaggtcc ggcaagttcg gaaaacttcg aagcgatcgc tttaaagatg tcccaatcag
     1501 ccttgctctc ccagagggga ttcacggcct cgcacaacgg gtggatgaag gggtgcatgt
     1561 ccgacgtgtt cagatcgtcc ttttcatacc aggttgcgct gggcaacacg atatcggagt
     1621 agaggcaggt cgaggacata cgaaaatcaa gcgtcacaag cagatcgagc tttccagtgg
     1681 gagcctcatc acgccagacc acctcgcgag ggagcggcgc ttcctgttcc ttgagatcgg
     1741 gagccaggat gccgtgagcg gcgccgagca gatgcttcaa aaag
//
LOCUS       DATMTI010000312         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_11855, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000312 DATMTI010000000
VERSION     DATMTI010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11855"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..453
                     /locus_tag="VN039_05210"
     CDS             301..453
                     /locus_tag="VN039_05210"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HWV45421.1"
                     /translation="MGTLLLIILILFLVGALPTWPYSANWGYGPSGGLGVVLLVLIIL
                     VLTGRM"
     gene            1012..1245
                     /locus_tag="VN039_05215"
     CDS             1012..1245
                     /locus_tag="VN039_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWV45422.1"
                     /translation="MNAEQLKGKWKQFKGELKEKYGKFTDDDLKQIEGNYEKFVGKAQ
                     ERYGDKKDALMKWADQWHEKPAPKPVGKGQKPR"
     gene            1305..1859
                     /locus_tag="VN039_05220"
     CDS             1305..1859
                     /locus_tag="VN039_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWV45423.1"
                     /translation="MMHIRYVLTLCTALVFSGALIAGSVHALDKANEKTPINDTWLTA
                     KTKIALAADGRVKGRQIDVETTQGVVLLRGKVDSGAAKQASADIAKMLDGVKSVKNDL
                     EVVAPAAREAVEDKDDAITTHVKEQITKDANLKKADIAVQTNAGVVSLTGEVRDLMTS
                     ANASWTVRQVAGVKSVKNDLTVKE"
     gene            1954..2250
                     /locus_tag="VN039_05225"
     CDS             1954..2250
                     /locus_tag="VN039_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45424.1"
                     /translation="MKKLIGSFVFALVLGFGMASLSFAAGDMAAPSTQTVKGDLLKID
                     GEFYVVKDMAGQEIRLHVDKTSQLDGTFKAGDKIEAQATDKGHASSIKQAKPKM"
BASE COUNT          588 a          565 c          670 g          485 t
ORIGIN      
        1 aagacgcgac gcgcgtaccg atgagacatc agaaggtcgc ctgaactgga gtcgccgaga
       61 tccgggcatc atcagagaga cggggggaca cgattctgcc gtttttccaa ggctagagcc
      121 gtgatgcccc agtgagcgtc atgccagtgc gaccacgaag gtagaaggag gatatatgct
      181 gccaacgctg tcgagggcta ctggtatgcg aaacctttga tgacctgaac gtcgaaacac
      241 acctagccgg taggtgaagg tattgtacta ggttaagaca ttttttaacg gaggtgacat
      301 atgggtaccc tactcctcat catcctcatt ctattcctcg tgggcgcgtt gcctacgtgg
      361 ccctacagcg cgaactgggg ttatggcccc agcggcggac tgggcgtggt cctgttagtc
      421 ttgatcattc tggtgttgac tggtcggatg taaccggcgg cggaaaagct ttgtcggcca
      481 gtagccggcg aagttctgct gtgcctatgt gatttttgca acgggctagt cgttccgcac
      541 ataatcacta cggccgacca cccacagcat cacgcatgac ttgaggacct ctcctcgtcg
      601 atctcctcgg actcgaaagc cgcatccctc acagtcttgc ggctgtgcag atcgtcttct
      661 ctgaaccgag gcggtttcgt tacttgtccc tcctgttccg aagagcattc tcgagctgat
      721 agtgctccac tgaatgatga caaaggccag gtagccggag cgtgcagcct gttgtcggcc
      781 caattctgga acgtcatcga gaccgaccag ccgacatcat cgacactaca cgggcacgta
      841 tcacgcgaaa gtaacgcctc atgctaccca aatcattcat ttcttgccac atgatcaatt
      901 ctgaccagac cgccgccggt cttctagtca taatggcttg ttatggaggg aggtttggat
      961 gtgccgggac agattgaata cggacacgca taaatacagg aggaagacat tatgaatgca
     1021 gagcaactca agggaaaatg gaagcagttt aagggggaac tcaaagagaa atatggcaag
     1081 tttactgacg acgacttgaa gcaaatcgaa ggcaattacg aaaagtttgt cggcaaagcc
     1141 caggaacggt atggcgacaa aaaggatgcc ctcatgaaat gggcggacca gtggcatgag
     1201 aagccggcgc cgaaacccgt ggggaaggga caaaagcccc gctgaatgga tgcaatttac
     1261 gaaagacctc tgacgacaca cgttagattg cgcttaagga gaacatgatg cacatacgct
     1321 atgtactgac actttgcacg gctttggttt tcagtggggc acttatcgct ggatcggtgc
     1381 atgcgcttga caaggccaat gaaaagactc cgatcaacga cacgtggctg acggcgaaaa
     1441 ccaaaatcgc tctggcagct gatggtcggg tcaaaggaag acagatcgat gtcgagacca
     1501 cgcagggtgt tgtgctgctt cgcggcaagg tggattcagg tgcagccaag caagcgtcgg
     1561 cggacatcgc caaaatgctt gatggcgtca aaagtgtgaa gaacgacctt gaggtcgtcg
     1621 ccccggctgc acgcgaagcg gtggaggaca aagatgacgc cattacgaca cacgtgaagg
     1681 aacagattac gaaagatgca aatttgaaga aagccgatat tgccgttcag accaatgcag
     1741 gagtcgtctc gctgaccggc gaagttcggg acctcatgac gagcgccaac gcgtcctgga
     1801 ccgtccggca ggttgccggt gtaaagtccg tcaagaatga tctcacggtg aaggagtagg
     1861 cctgaagaag ctctgggaat cgattcgctc gttttggttc gaatgagcga atcggcttaa
     1921 ttacattcac aattaattac aatgaggttc gtcatgaaaa agctcatcgg ttcgttcgtg
     1981 tttgcgctgg tcctcggttt cgggatggca tcactctcct tcgcggctgg tgacatggcc
     2041 gcgccaagca cccagacggt caaaggggat ctgctgaaga tcgatggaga gttttacgtg
     2101 gtgaaggata tggccggcca ggaaatccgt ttacatgtcg acaaaacctc tcagctggac
     2161 ggcaccttca aggcggggga caagatcgag gcccaggcca cggataaggg ccatgcctcg
     2221 tccattaagc aagccaagcc gaagatgtag gttggagata cggacggaga agttcaggca
     2281 gagagattgg gccagtcttc aaggctgg
//
LOCUS       DATMTI010000313         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_309285, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000313 DATMTI010000000
VERSION     DATMTI010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309285"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2347
                     /locus_tag="VN039_05230"
     CDS             <1..2347
                     /locus_tag="VN039_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV45425.1"
                     /translation="TMARPLSQARIPGAGRETAAKLQRDDFFAEAFRLSSHLIGITEL
                     ETGVCLEINDACLAIFGFRRDEVIGKTTLMLGIWPDPHDRARLIDRLKSKGSIRNLEV
                     SMRMKNGELRQFLISADLMTLGGKPCLLTIGNDITERKRVEETLRRTHDELEQRVRKR
                     TADLEQTNAAMRDSEDRFRLFIEHAPAAIAMFDRDMRYLAASRRWIEEYRLAGDILGR
                     SHYDVCPEVSARWKEVHRRGLAGDVLSADEDRFVRSDGTTQWITWDVRPWFCGDDISG
                     IVIATEDVTARVEAKHALHEREERSDQVIRLAHFGILDHDHRTGKVYWSPAMREIYGV
                     GPDDPASMEEYMQLLHPEDRETVLLAIKQAQDPAGDDLFSVEHRLLRRDGTVRWVNFR
                     SWTLFDNEGPERRPTRTLGAMIDITKRKHAEEALRMSERRFTSFMDNLHGFAWIKDAE
                     GRHLYVNRLFEESVLNGLDWKEKTARELWSEEIAVQYELNDRKVRETRSPLHTVEQFV
                     QNGEVRHALVSRFPIADHKGAPVLLGSVAVDITERKQAEEALHRNQLELHQQQVQLEE
                     LTSKLLTAQEHERQRIARDLHDDVSQRLAVLVLEVASLEHHPCAVPAELTRALGPLRE
                     QLEHLSDDVHTLAYRLHPSLLEHAGLRPAVEDHVQQVSRRTGLPIHLKILDVPNAVPL
                     NQATCLFRVMQESVQNVVKHAQATTVTVQLRGSSKGVGLSVIDNGKGFDPQDLLTHQQ
                     GLGLSSMEERLRQLHGFFRIQSRSSHGTKVCAWVPCGVEVA"
     gene            2344..2997
                     /locus_tag="VN039_05235"
     CDS             2344..2997
                     /locus_tag="VN039_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV45426.1"
                     /translation="MKRPRILMADDHAIVLAGLRKLVEAESEVVGMVEDGRSLVDAAQ
                     QLRPDIVLLDISMPLLNGLDAARQISKLVPESKLIFLTMHATPTYATEAFKAGASGYL
                     IKRSAAVELKQAIQAVMQGQHYMTPLITKDVLAATLQSPEDQPSKPLVTHLTQRQREV
                     LQLVAEGKGTKAIASILNISVKTVEFHKFRIMGELDLHSTAELIKYAIAEGLVSVSS"
BASE COUNT          705 a          805 c          911 g          636 t
ORIGIN      
        1 gaccatggcg cggccgcttt cacaggctcg catccccgga gccgggaggg agactgccgc
       61 caaactacaa cgggacgatt tcttcgccga agccttccgc ctaagttctc acctgatcgg
      121 tatcacggaa cttgaaaccg gggtctgtct ggaaatcaat gacgcctgtc tagccatatt
      181 cggtttccgc cgggacgagg tgattggaaa gacgacgttg atgttgggta tctggcctga
      241 ccctcatgat cgggccaggc tcatcgatcg attgaaatcc aaggggtcaa ttcgaaatct
      301 tgaagtgtcg atgcggatga agaacggtga attgcggcaa ttccttatct ccgcggactt
      361 gatgacgctc ggagggaaac cgtgcctcct gacgatcggc aacgacatta cggagcgcaa
      421 gagggttgag gagacgctgc gtcgaacgca tgatgaatta gagcagcgcg ttcggaagag
      481 aaccgcggat ctcgagcaaa ccaatgccgc gatgcgggac agtgaggatc gcttccggtt
      541 attcatcgag cacgcaccgg ccgcgatcgc gatgtttgac cgcgacatgc gctatctggc
      601 ggcgagccgc cgctggatcg aggaataccg gctggcaggg gatatcctcg gtcgatcgca
      661 ctatgatgtg tgtcccgagg tttcagcacg atggaaggaa gtccaccgcc gaggtctggc
      721 tggagacgtg ctgagcgcag atgaagaccg gttcgttcga agtgacggca caacgcagtg
      781 gatcacttgg gatgttcgcc cctggttctg cggcgacgac ataagtggaa ttgtgattgc
      841 cacggaagat gtgacggctc gggtggaggc gaaacacgca ctgcatgaaa gggaggaacg
      901 gtccgatcag gtcattcggc tggcccactt cggcattttg gatcatgacc accgcaccgg
      961 aaaagtgtac tggtctccgg caatgcggga aatctacgga gtcggacccg atgatcctgc
     1021 ttccatggaa gagtatatgc agttactcca tcctgaagat cgcgaaacgg tcctcctggc
     1081 catcaagcag gctcaagatc ctgccggtga cgatctcttc tcggtagaac accggctgtt
     1141 gcgccgcgac ggtacggtca gatgggtgaa ttttcgatca tggacgttat tcgacaacga
     1201 ggggcccgag cgtcgcccta ctcgaacttt gggggcgatg atcgacatca cgaaacgcaa
     1261 acatgccgag gaggctctca gaatgagcga gcgtcgattc acgtccttta tggataattt
     1321 gcacggcttt gcgtggatta aagacgctga gggacgacac ctgtacgtca atcgactctt
     1381 tgaagaatcc gtcctcaatg gattggattg gaaagaaaag acggcccgtg agttatggtc
     1441 tgaggagatc gccgtacagt atgagttgaa cgataggaaa gtgcgggaaa caagatcccc
     1501 tctgcacaca gtcgagcagt tcgtccagaa cggtgaggtc cggcacgcgc tggtgagcag
     1561 gtttcccatc gccgatcaca agggagcgcc ggtgctgttg ggaagcgtcg ctgtcgacat
     1621 caccgaacgc aagcaggcgg aagaggccct tcatcgtaac cagctggaat tgcatcagca
     1681 gcaggtgcaa ctggaagagc tgacgtccaa gctgctgacg gcacaagagc atgaacgaca
     1741 gcgaatcgcc cgggatttgc acgacgatgt gagtcagcga ttggctgtct tggtcctgga
     1801 agtggcatcc ttggaacacc acccttgcgc cgtacccgct gaacttactc gagcgctggg
     1861 gccactccgt gaacagttgg aacacctttc cgatgatgtg catacgctcg cgtaccgact
     1921 tcatccatct ctgttggagc atgctggatt gcgcccggcg gtcgaagacc atgtccagca
     1981 ggtatcccgg cgcacgggct tacccattca tctgaagatc cttgatgttc caaatgcggt
     2041 accgctcaat caggcgacct gtctcttccg cgtcatgcag gaaagcgtcc aaaatgtggt
     2101 gaaacatgcg caggcaacga ctgtgacggt ccaactccgc gggtcgtcga aaggggtcgg
     2161 gctgtctgtc attgacaatg ggaaggggtt tgacccacag gaccttctta cccatcaaca
     2221 gggactggga ttgagcagta tggaggaacg gctgcgacaa ctgcacgggt tctttcggat
     2281 ccaatctcgg tcatcccatg gcacaaaggt ctgtgcttgg gtgccttgcg gggtggaggt
     2341 cgcatgaaac ggccccgaat tctaatggcc gatgatcatg ccattgtcct ggccggactt
     2401 cggaagctgg tggaagccga gagcgaagtg gtcggtatgg tggaagacgg acgatcactg
     2461 gtcgatgcgg cccaacagtt acgccccgac attgtgttgc ttgacatctc gatgccgttg
     2521 ctcaatggat tagatgcagc acgccagatc agtaagctgg tgccggagag caagctgatc
     2581 ttcctgacca tgcatgccac ccccacctat gcgaccgaag cttttaaggc cggagcctcc
     2641 ggctatttga taaaacggtc cgccgcagtg gagctcaagc aagccatcca ggcggtgatg
     2701 cagggacaac actatatgac accgctgatc acgaaagatg tcttggcggc cacgctccaa
     2761 tccccggagg accagcccag caagcctttg gtgacccatc tcacacagcg gcaacgagag
     2821 gtgttacagc tcgtcgccga ggggaaaggt accaaggcta ttgcctcgat tttgaatatt
     2881 tccgtgaaga cggtggaatt tcacaagttt cgcattatgg gcgaactcga tctccactcg
     2941 accgctgagt tgatcaaata tgcgattgcc gaaggcctcg tgagcgtgtc gtcataagcg
     3001 cgttctggtt ttgcatactg cccgaggtac aatctggact acctaataca acatcat
//
LOCUS       DATMTI010000314         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_138931, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000314 DATMTI010000000
VERSION     DATMTI010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138931"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..137
                     /locus_tag="VN039_05240"
     CDS             18..137
                     /locus_tag="VN039_05240"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWV45427.1"
                     /translation="MPKFVKTYAHLKADTLQALSRYKEEVEQGKFPSDSESYH"
     gene            274..1050
                     /locus_tag="VN039_05245"
     CDS             274..1050
                     /locus_tag="VN039_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWV45428.1"
                     /translation="MDWVDWLLSQEVRQFVFDHHLVYPLVFVLRLFGVTALELFRPAR
                     TVPYRRLIGRDILLISIYWFGMVPIAESIDRLIAIRPHLPQAIHEMPLLLRLFCYFLI
                     ADFGHYWIHRLMHMKHVWRIHKWHHSPAHMYWLAGFRATIPQDVIVNLPYLFAYSFLD
                     LSPSWMELAIGIFNNLQNDWMHANIVWRSNWLEWLIVTPRYHHIHHSDKPVHYMANLG
                     TLFTVWDRLFGTYVDPESVKGELSFGIGEKVPPLRLVLGV"
     gene            complement(1066..1881)
                     /locus_tag="VN039_05250"
     CDS             complement(1066..1881)
                     /locus_tag="VN039_05250"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HWV45429.1"
                     /translation="MATYAIGDVQGCHDALQRLLDHIQFDPSCDRLWFVGDLVNRGPD
                     SLGVLRYIRSLGPSARVVLGNHDLFLLAVSEGAAALRPKDTIGDVLKADDRPDLIDWL
                     RHQPLHQREGLFFMIHAGLVPQWTTQDAAQLACEVETALRGDGFQSFLRSLFHEPAVT
                     WSPNLTGASRLVTVARILTRLRACTPTGDTLNFSGPPEEIPPGYFPWFRLPARRSADT
                     TIITGHWAALGLHMEPNLLAIDSGCVWGRQLTAVRLEDRRVFQVNCVDAVRRA"
     gene            complement(1982..2383)
                     /gene="queF"
                     /locus_tag="VN039_05255"
     CDS             complement(1982..2383)
                     /gene="queF"
                     /locus_tag="VN039_05255"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWV45430.1"
                     /translation="MKDKQKTTKLGYNERHAKSGISAPLPDIETFPNQYKGYEITIEI
                     PEYTAICPKTNLPDFGTITLHYMPDKECLELKALKMYIHAYRDLGIFYENAVNRILQD
                     IIKACRPVWAKVTGTFTARGGLSSKIEARYP"
BASE COUNT          514 a          665 c          668 g          603 t
ORIGIN      
        1 gactctttga tgcgttcatc ccgaaattcg tgaagaccta cgcccacctc aaagccgaca
       61 ccctccaagc gctgagtcgt tataaagaag aggtggagca aggtaaattc ccgagcgatt
      121 ccgagagcta ccactagctc ctctttccct tgcgattggg ttaggtcgct cactcatcgt
      181 gcgcgcttgc cagatccgat tcgtcaattt ccacagaaag cacgacaggt cataccgaat
      241 acgataaggt gcgccataaa gagggtcgcc ttgatggatt gggttgactg gctcttgagt
      301 caggaagttc ggcagtttgt tttcgaccac catcttgtct atccactcgt tttcgtcctg
      361 cgtctgttcg gcgtgaccgc tcttgaactg tttcggccgg cgcgaacggt cccctatcgt
      421 aggctcatcg ggcgggatat tctcctgatc tccatctatt ggttcggcat ggtcccgatc
      481 gccgagtcta tcgatcggct tatcgccatc cgcccgcact tgcctcaggc cattcacgag
      541 atgccgttgc tgcttcgcct gttctgctac ttcctcatcg cagatttcgg gcattactgg
      601 attcatcggt tgatgcacat gaagcatgtc tggcggattc ataagtggca ccattcgccc
      661 gcccacatgt attggctcgc gggctttcgc gcgacgatcc cgcaagatgt catcgtcaat
      721 ctgccctatc tgtttgccta ttcattctta gacctctctc cttcgtggat ggaacttgcg
      781 atcggcatct tcaacaatct gcaaaacgat tggatgcatg ccaacattgt gtggcgctcc
      841 aactggctcg aatggctgat cgtgaccccg cgctatcacc acattcatca cagcgataag
      901 cctgtgcact acatggccaa tcttgggacg ttgtttactg tctgggaccg tttgtttggg
      961 acttatgtgg atccggagtc agtgaaggga gagctttcgt tcgggattgg agagaaggtg
     1021 ccacctcttc gtcttgtatt gggtgtctag aatatgtgcg tgagcctacg ctcggcgtac
     1081 cgcatccacg cagttcacct gaaacaccct gcgatcttcc aatcgcaccg ctgtcagttg
     1141 ccgtccccac acacaaccgc tatcgatggc aagcagattg ggctccatgt gtaggccgag
     1201 tgcagcccaa tggccggtaa tgatggtcgt atccgcactg cggcgagcgg ggagacggaa
     1261 ccaagggaaa tagcccggcg gtatctcttc cggcggaccg gaaaagttca aggtatcgcc
     1321 ggtcggtgta caggcgcgaa gtcgggttag tatacgggca accgtgacca accgtgaggc
     1381 gccggtaagg ttggggctcc acgtgaccgc cggctcatgg aagagtgatc gcaagaacga
     1441 ttggaagccg tcgccgcgca gtgcggtctc gacctcgcag gccaattgag cggcatcttg
     1501 cgttgtccat tgagggacta gccccgcatg gatcataaag aataaccctt cgcgctgatg
     1561 caggggttgg tggcgaagcc agtcaatcaa gtccggccga tcgtcggctt tcaatacgtc
     1621 gccgatcgta tctttggggc gaagcgccgc cgccccttcc gagactgcaa gaagaaagag
     1681 gtcgtggttg ccaagcacga cgcgtgcgga tggtccgagc gaacggatgt agcgaagcac
     1741 accgagggaa tcgggcccgc ggttgacgag atcgccgacg aaccaaagcc ggtcgcagct
     1801 cggatcaaat tggatatgat cgagaagacg ttgtaaggca tcgtggcacc cctgcacatc
     1861 gccgatcgca tacgtcgcca taaaaaagaa atgtcaacgg tcagtggtca ttcgtcactc
     1921 acaaaagaga ataggctgac ttttctttgt aatgacggac gaccagtgac caatgacggg
     1981 attaaggata ccgagcctca atcttgcttg aaagcccgcc gcgtgccgtg aaggtgccgg
     2041 tgaccttcgc ccatacggga cggcaggctt tgatgatatc ttgaaggatt ctgttgacgg
     2101 cattttcata gaagatgccg agatcgcgat aggcgtggat gtacatttta agcgctttca
     2161 gctcaaggca ttctttgtcg ggcatgtagt gcagtgtaat ggttccgaag tccggcaagt
     2221 tggttttggg acaaatcgcc gtatactcgg ggatctcaat cgtaatttca tatcccttat
     2281 attgattcgg aaaggtctcg atatcgggta gcggggcgct gattccgctc ttcgcatgcc
     2341 gctcgttgta ccccaatttc gtggtctttt gcttatcctt catttaccct ctccgctggc
     2401 ccgctcatac ttgtttcatc cattcagtct ggccgatttt ctccagctct
//
LOCUS       DATMTI010000315         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_818377, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000315 DATMTI010000000
VERSION     DATMTI010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818377"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1210)
                     /locus_tag="VN039_05260"
     CDS             complement(146..1210)
                     /locus_tag="VN039_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031313474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWV45431.1"
                     /translation="MRSKAVVLSETDRATLGRMSRMGKAAQRDVLRARIILLTAAGGS
                     DLAVAEQLRINRHTVRLWRQRFAEKGLEGLTDTPGRGRKPILTHGVTESILTGAVQPP
                     PNRTRWSCRSMAKAHKVSKSTVQRLWSKNDIKPHVTRTFKISTDPRFEEKFWDVIGLY
                     LDPPDKALVLCCDEKSQCQAIERTQPGLPLGVGHIKTATHDYIRHGTVTLFAALNYLE
                     GKIVSMLAEKHRHQEWLKFLKRIDRETPAGLELHLIADNYATHKHADVQKWLAKHKRF
                     HMHFTPTSASWMNLVERFFRDLTVDVVREGSFKHVKELGDQILAYLAERNAQPTRYVW
                     KAKGEEILRKIHRARQVLTV"
     gene            1332..>1632
                     /locus_tag="VN039_05265"
     CDS             1332..>1632
                     /locus_tag="VN039_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWV45432.1"
                     /translation="MKSPVPAYRRHDLSDETWKRLEPHLPGRKGAWGGVAQDNRLFIN
                     AVFWILRTGAPWRDLPPGYGGWSNTHRRFIRWRDAGIWEKLLERLIDEPDYEWLMI"
BASE COUNT          315 a          468 c          469 g          380 t
ORIGIN      
        1 ctcctatgct caaaatgtga ttcttgtagg agtacttcac agggaagagg atgacgtagt
       61 gagtcagtac gtagccacct gtgacggtgg ggaccatgaa tacatcacca agaagacggc
      121 ttctagtgtg gtgtctctca aatagctata cagtcagcac ctgcctggct cggtgaatct
      181 tgcggaggat ctcttccccc ttggctttcc agacgtagcg cgttggttgt gcatttcgtt
      241 cggctaggta cgccagaatc tggtcgccga gttccttgac gtgcttaaag ctcccctctc
      301 gcacgacgtc gacggtgagg tctcggaaaa agcgttcgac caggttcatc cacgacgccg
      361 atgtcggcgt gaagtgcata tgaaacctct tgtgcttggc caaccacttt tggacgtccg
      421 catgcttgtg cgtagcgtag ttgtcggcaa tcagatgcag ctctaacccc gcgggcgttt
      481 cgcgatcgat ccgtttgaga aacttcagcc attcctgatg gcggtgtttc tctgcgagca
      541 tcgacacgat cttgccttcc agataattga gcgcggcaaa cagagtcacc gtgccgtgcc
      601 ggatatagtc atgggtggct gtcttgatgt gccccactcc cagtggtaag ccaggctggg
      661 tgcgctcgat cgcctggcac tgacttttct cgtcgcagca gagcaccagc gccttgtcag
      721 gaggatccaa gtacaagccg atcacatccc agaacttttc ttcgaatcgc ggatcggtgg
      781 agatcttgaa tgttctcgtc acgtgcggct tgatgtcgtt ctttgaccag aggcgttgga
      841 ccgtgctctt ggagaccttg tgggccttgg ccatcgaccg acaactccat cgcgtgcgat
      901 ttggtggcgg ttgcacggcc cctgtcagaa tcgactcggt cacgccgtgg gtgagaattg
      961 gcttacggcc acggcccggt gtatcggtta acccctccag tcccttctcg gcgaaccgtt
     1021 gccgccacaa ccgcaccgtg tgtctgttga tccgcaactg ctcagccacc gccagatccg
     1081 acccgcccgc ggctgtcagg aggatgatcc gcgcccgcag gacatcccgt tgcgcggcct
     1141 ttcccatccg actcattcgc cccagcgtgg cccggtccgt ctcgctcaac accacagcct
     1201 tggatctcat agcccggcag tatatcccca tcagcccata atgtctatcc atttatggga
     1261 cactagacta gggcgtgtag tcacgagatt aaagattatg agaggatcat ggggccagag
     1321 aaggaggcca catgaagtcc ccggttcctg cctatcgccg tcacgatctc tccgatgaaa
     1381 cctggaagcg gctggagccg catttgccgg ggcgcaaagg cgcctggggc ggcgtggccc
     1441 aggataaccg cctgtttatc aatgcggtgt tctggatttt gaggaccggt gcgccctggc
     1501 gcgacctgcc gccgggatat ggcggatgga gcaatacgca tcgtcgcttt atccgatggc
     1561 gcgatgcggg catctgggag aaacttcttg agcgtctgat cgacgagccg gattacgaat
     1621 ggttgatgat cg
//
LOCUS       DATMTI010000316         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_309356, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000316 DATMTI010000000
VERSION     DATMTI010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309356"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..997
                     /locus_tag="VN039_05270"
     CDS             146..997
                     /locus_tag="VN039_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45433.1"
                     /translation="MEFPLQFSELTIPTGAGPEQQRITINHGNDGEIKVYNSSGNLVD
                     ALGGPNGQFIVYDNNGNMVISLASASGTDQKTGNTYDAGITTYSSGNTLNLFDAVGTW
                     TASDGSQVIVEAGTGATIQLQPENAALGVGPWFNAQIFTSIAGGGHPAVEIDSPSQQG
                     TGIASSIQLQGSSTNSGLTQIFYAADQHHFNSGTVDMTGSVVEMGNVAIGGTVITPTA
                     ANTPTKGTVNFSALSGTTAHGFAVAQNSNANTFVSITNIGTTSADIWISRPNTTATGV
                     YYAVWYT"
     gene            1071..1319
                     /locus_tag="VN039_05275"
     CDS             1071..1319
                     /locus_tag="VN039_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45434.1"
                     /translation="MASVQTHIGAKPIGDNDTGPQFMIGFTSEHFSQCVFLDVPTDPK
                     DAVIMADAFYKGILEACAVAHKEWNSNKDADAPKDGTA"
     gene            1501..2001
                     /locus_tag="VN039_05280"
     CDS             1501..2001
                     /locus_tag="VN039_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45435.1"
                     /translation="MWVNIIKTTEGKYVAQARITHASHHPVFETIMYETVEYTTLRMA
                     QADAKCWIAFHGRNAKVDTETIEVMDDDSVYTSSGNRVTGKKFKEILGMLQNNLVERG
                     EEPRKLHYIPANDSDRAFHIEFDNGNHFVTVHRASRDSGSAVAEFIGGTRIAKTPGAV
                     SIASGD"
     gene            2097..>2301
                     /locus_tag="VN039_05285"
     CDS             2097..>2301
                     /locus_tag="VN039_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45436.1"
                     /translation="MGNADIITVGYDEWDNYIDVRCVMCCKVVSPVGAPNFEVPNVVL
                     FPDGRRGDDYTEAGLCEGCARELR"
BASE COUNT          580 a          659 c          600 g          462 t
ORIGIN      
        1 ccgctgcggg gctgccatac ctgtatctac aagcctctga cgaggttgag atagtcatca
       61 gtgccgcaac caacgaggat caagttgctg tgcgagcgca gtacagggaa atagactcgt
      121 ccaatcctct cgtccaagga atgtgatgga attcccactt cagttttcgg aactcacgat
      181 acccacgggc gcgggaccgg aacagcaacg catcaccatc aatcacggca acgacggtga
      241 aatcaaggtc tacaactcta gcggcaatct cgtcgacgca ttgggcggac ctaatggtca
      301 gttcatcgtc tacgacaaca acggcaacat ggtgatctct ctcgccagcg cttccggaac
      361 agaccaaaag accggcaata catatgacgc cggaatcaca acctactcaa gcggcaatac
      421 cctgaacctg tttgatgccg tcggcacatg gaccgcttca gatggctcac aggtgattgt
      481 tgaagctggc acaggcgcta cgattcagct tcaacctgaa aacgccgccc tcggagtagg
      541 cccatggttc aacgcccaaa tattcactag cattgcaggc ggcggacacc ccgccgtaga
      601 aatcgattct ccatctcaac agggaacggg aatagcgtca tccattcagt tgcagggatc
      661 cagcaccaat agtggactga cgcaaatctt ctatgcagcc gaccaacacc acttcaacag
      721 cggcaccgtt gatatgacgg gcagtgttgt ggaaatgggt aatgtcgcta tcggcggtac
      781 cgtcattacc cccacggctg cgaacactcc cacaaagggg accgttaact tctcagctct
      841 tagtgggact actgcacacg ggttcgctgt agcacagaac tcaaacgcca acacattcgt
      901 cagcatcacc aatatcggca ccacgtcggc ggacatatgg attagtcgcc ccaacacgac
      961 cgctacagga gtctattacg cagtgtggta cacgtgatca aagtactctg tttcatcaca
     1021 gcattcgtgg caggctttct catcatctca ggactaaccg cgaggtaatc atggcaagcg
     1081 ttcaaaccca catcggtgca aagcctatcg gcgacaatga caccggcccc caattcatga
     1141 ttggtttcac gtctgagcac ttctcacagt gtgtcttcct tgacgttcct acagacccta
     1201 aagacgcagt catcatggct gacgccttct ataaggggat cctggaagca tgcgccgtag
     1261 cgcacaagga gtggaactcc aacaaggatg ctgacgcacc caaggacggc acggcctaga
     1321 ctcgcggtaa ggccgtgata gtcggttagc gtaactacct ggggcgcgac gatccgacta
     1381 gggcaccaat cacagcaact cccatcattg ctgtgattgg tgcctttccc tgtgttgact
     1441 tcaatcgtgc caaccactag tgtgtctttt gtaagggaga caacggcaag ggagcgagcc
     1501 atgtgggtca acatcatcaa gacgaccgaa ggaaagtacg tcgcacaggc tcgcatcacc
     1561 cacgcgtcac accaccccgt cttcgagacc atcatgtacg agaccgttga gtacaccact
     1621 ctccgcatgg cacaggccga cgcaaagtgc tggatcgcat tccacggaag gaacgccaag
     1681 gtggacaccg agactattga ggtcatggac gacgacagcg tctacacgag ctcgggaaac
     1741 agggttaccg gaaagaagtt caaggagatt ctggggatgc ttcagaacaa cttggtcgag
     1801 cggggtgagg agccgcgcaa gctgcactac attcccgcca acgacagtga cagggcgttt
     1861 cacatcgagt tcgacaacgg caaccatttc gttaccgtcc accgtgcaag ccgtgacagt
     1921 ggaagtgcgg tagcggaatt catcggcggc acaaggattg ccaagacccc tggcgcagtc
     1981 tccatcgcga gtggcgacta gcatgacttg tgaggcagtc tcgcaacacc tcttgtgaga
     2041 ctgcctcact ctctgatagt gttctttaat cagcacatcg aaccagggag cagatcatgg
     2101 gtaacgcgga catcatcaca gtcggatatg acgagtggga caactacatc gacgtgcgct
     2161 gcgtcatgtg ctgcaaggtc gtatcacctg tcggcgctcc taactttgag gtcccgaatg
     2221 tagttctctt ccctgacggc cgacgcggtg atgactacac tgaagctggt ctctgcgagg
     2281 gttgcgcacg cgagttgcgc g
//
LOCUS       DATMTI010000317         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_139002, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000317 DATMTI010000000
VERSION     DATMTI010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139002"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VN039_05290"
     CDS             complement(<1..674)
                     /locus_tag="VN039_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV45437.1"
                     /translation="MDRRRPIAVLVERMPPDMPITTNIPQRMDRLPWARWHWLVVGAL
                     GITWLLDGLEVSIVASLGPTLTHPDTLHLTQSEVGLTASVYLAGSVLGAFLFSYLTDR
                     QGRKKWFMITLALYLTATVLTAFSWNLMSFLFFRFLTGAGIGGEYAAINSAIDELIPA
                     RNRGHTDLAINGTWWLGSAAGALMTLLLLNPAIFTESIGWRLCFVLGAVLGVAVILVR
                     RFIPESP"
     gene            complement(687..2150)
                     /gene="zwf"
                     /locus_tag="VN039_05295"
     CDS             complement(687..2150)
                     /gene="zwf"
                     /locus_tag="VN039_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWV45438.1"
                     /translation="MMKETVEPHLFIILGATGDLTRRKLLPALFHLRTYGEQEKQNTL
                     IVGAALPEMSVDAFRLWAYEGLRNSGARDASGLRRWCEDQLYYQPLREGRLSDYQALA
                     AFIARLEVARNLPQNRIFYLALPPTIVPETLELLDQAGLLKSRGWVRAVLEKPFGHDF
                     HSARALNTLVHRYLDESQIYRIDHYLGKETVQNLLAFRFANPIFESLWNRNTVENVEI
                     TVAEDLGVEHRGAYYQKAGAFRDMVQNHLTQLLTVVGMEVPTSFEASEIRAEKLKVLR
                     SISSIRPQDVIFGQYTAWDVAGQRIPGYLEEPGVPPDSTTETYVALKLEIHNWRWRGV
                     PFYLRTGKRLPRKITQVAVTFRAAPTQVFRSLAPGSLNPNKLLITLQPSEGFSLCFSV
                     KTPGRPFKFTDRALRFDYSQGFGELPEAYETLLRDVMIGDQTLFVTADFTETAWRLYD
                     PLLLDPRPVHLYTAGSWGPKEADVLVERNGLNWQLGW"
     gene            complement(2252..2482)
                     /locus_tag="VN039_05300"
     CDS             complement(2252..2482)
                     /locus_tag="VN039_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45439.1"
                     /translation="MKNRSAREEEPMSPYRWSPNKPTRPGWYWFRGLAHEADPFIVLV
                     DQAGEFQWPDGGFQEVSLANGEWAGPIEEPKE"
BASE COUNT          592 a          737 c          702 g          553 t
ORIGIN      
        1 ggactttcag gaatgaaccg ccgaacgaga atgacggcca ctcccagcac ggcaccgagg
       61 acaaaacaga gccgccatcc gatggattca gtgaagatcg caggattcaa caagagcagt
      121 gtcatcaagg cgccggccgc cgatccaagc caccaggttc cgttgatcgc cagatcggta
      181 tggccgcgat tgcgcgcggg aatcaattca tcgatcgcgg aattgatggc cgcgtactcg
      241 cccccgatgc cggctccggt cagaaatcgg aagaacagga agctcatgag gttccatgaa
      301 aatgccgtga ggaccgttgc cgtgagatac agcgccaagg tgatcatgaa ccatttcttt
      361 cgtccctgcc gatcggtgag ataggagaag aggaacgcgc cgaggaccga acctgccaga
      421 tagacggacg ccgtgagccc tacttccgat tgcgtgagat gtaatgtgtc cgggtgggtc
      481 aacgtcgggc cgagtgaggc cacgatcgat acttcaagcc cgtcgagcag ccaggtgatg
      541 cccaatgccc ctacgaccaa ccagtgccag cgtgcccatg gcaaccggtc catccgttgg
      601 ggaatattgg tggtgatcgg catatcggga ggcatgcgct ctactaatac ggcaatcggt
      661 cgccgtctgt ccaatgcttg gagcgcctac caacccaatt gccagttcag cccgttccgt
      721 tccacgagca cgtccgcttc ctttgggccc cacgagccgg ccgtgtagag atgcaccggt
      781 cggggatcga gcaacagcgg gtcgtagagt cgccaggccg tttccgtaaa atctgcggtg
      841 acgaataatg tctggtcacc gatcatgacg tcgcgcaaca gcgtctcata ggcttccggc
      901 aattcgccga atccctgact ataatcgaat ctcagtgctc gatcggtgaa cttgaacggc
      961 cgcccgggag ttttcacgga aaagcagagc gagaagcctt cactcggttg caaggtgatc
     1021 aagagcttgt tggggttcag actacccggc gccaaagatc gaaagacttg agtgggcgcc
     1081 gctcgaaagg tgacggccac ttgcgtgatc ttgcgaggaa ggcgtttccc ggttcttaaa
     1141 taaaacggca ctcccctcca gcgccaattg tgaatctcca acttcagcgc cacataggtt
     1201 tcggtggtcg agtccggagg gacacctggt tcttccaggt agccgggaat cctctggccg
     1261 gcgacatccc acgccgtata ttggccgaag atcacgtcct gtggacgaat cgatgaaata
     1321 gagcgaagta ctttcagttt ttcagcccgg atctcggaag cttcgaagga agtcggcacc
     1381 tccatgccaa cgacggtcaa cagttgcgtt aaatggttct gcaccatgtc acggaaggca
     1441 ccggcttttt gatagtaggc tccacgatgt tcgacaccga ggtcttcagc gacggtgatt
     1501 tccacgttct ccaccgtatt gcgattccac agcgactcaa aaatggggtt cgcgaagcga
     1561 aaggccagta agttttgcac ggtctctttg ccgagatagt gatcgatgcg ataaatctgc
     1621 gattcgtcca gataacgatg tacaagggta ttgagcgcac gggcagaatg aaaatcgtga
     1681 ccaaagggtt tttccaagac ggcgcgcacc cacccgcggc ttttcagcaa gccggcttga
     1741 tcgagtaatt ccaaggtttc aggcacaatc gtcgggggaa gcgccagata gaaaatgcga
     1801 ttttgaggca gattcctggc aacttcaaga cgggcgataa acgcggcgag ggcttggtag
     1861 tccgacaatc tgccttcccg caggggctga tagtacagct ggtcttcgca ccatcgacgg
     1921 agaccggaag cgtcacgagc acccgaattg cgcagcccct cataagccca caagcggaac
     1981 gcatccacgc tcatttcagg caatgcggct ccgacgatga gcgtattctg cttctcctgc
     2041 tcaccatagg tgcgcaagtg aaacaatgcg ggcagcagtt tccgtcgcgt taaatctcct
     2101 gtcgccccca gaataataaa cagatgtggt tcgaccgttt ccttcatcat ccccgtgacg
     2161 attcctaaga tatactgaag ttatgactca ttttgtcagt cgtcaatgga cgccccctca
     2221 tcctccatcc cctttcacgt aaggcatcga tctactcttt cggttcctcg atcggaccgg
     2281 cccattcgcc gttggcgagc gatacctctt gaaatccgcc gtccggccat tggaactctc
     2341 ccgcttgatc gacaagcacg ataaacgggt cggcttcgtg ggccagcccg cggaaccagt
     2401 accagccagg ccttgtcggt ttgttagggc tccaacgata gggcgacatc ggttcttcct
     2461 cacgtgctga acggttcttc attcctcctt gtctccatgt tctggtagag tgggcaatct
     2521 ctattcatga ccctatcacg ccatgtccac acttccaata gaatacatac tgccgtcagt
     2581 ctgc
//
LOCUS       DATMTI010000318         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_394025, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000318 DATMTI010000000
VERSION     DATMTI010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394025"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VN039_05305"
     CDS             complement(<1..385)
                     /locus_tag="VN039_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HWV45440.1"
                     /translation="MGDRVKEILSWYGSDNAGTKTNIARLLRSGKLAGTGKLVILPVD
                     QGFEHGPARSFSANPPGYNPHYHFQLAIEAGCNAYAAPLGFLEAGASEFAGQIPLILK
                     LNNHDVLHDNKDPQPSVTGSVKDALR"
     gene            complement(429..1433)
                     /gene="fbp"
                     /locus_tag="VN039_05310"
     CDS             complement(429..1433)
                     /gene="fbp"
                     /locus_tag="VN039_05310"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HWV45441.1"
                     /translation="MREFPLTLGRFIIQNQTSHQDATEEFSSLLTQIGLVGKLISQDL
                     RRAGLINILGTTGDTNVQGETVKKLDAIANDDFVKVFQHSGYVCALASEEMEKPISIP
                     GNWPHGKYMLLFDPLDGSSNTDNNMPLGAIFSVLKYGRTDRLPTEEELLGRGTEQVAA
                     GYLLYGSSTMLVYTVGQGVYGFTLEPDIGEYLLSHEKIRIPEKGRVYAVNEGNYHKWS
                     EGTKKYLDSLKVSDKTTGRPYSSRYSGCLVADVHRLLLGGGIYLYPGEAGKPEGKLRL
                     LYEANPLAFVVEQAGGRASTGTSRVLEVEPKKLHQRVPLIIGSRQDVEQAEAHVQGKA
                     "
     gene            complement(1483..2319)
                     /locus_tag="VN039_05315"
     CDS             complement(1483..2319)
                     /locus_tag="VN039_05315"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWV45442.1"
                     /translation="MLTPVPTRFLRPPSVAQTCRYVVLVAVVALSSYSAGSIEAEPLG
                     ETKRPFQVGERLTYEVSWLNLTAVIAVMEVARIDGRKDQAVAKLVGTAQSTPVITKFF
                     PVDNRVESELDLDTLTPEHMTFRRREGKKKEDIEYVFHQKEGVVTAVRGGTTESLPIP
                     VGTQDIISCLYYTRTRLPPNPGASLKMNVYHDKKNRPVEVRVEAIETIEGAWGRAETV
                     RVLVIMPFHGLFMNKGNIRVWVTNDERKTPVRMTAKVVLGSIVADLVDGFEESGTAEQ
                     KY"
     gene            complement(2313..>3007)
                     /locus_tag="VN039_05320"
     CDS             complement(2313..>3007)
                     /locus_tag="VN039_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HWV45443.1"
                     /translation="ADRIGVVDEQGHVLWGDRLLVLYSRDILAMKPGSTIISEVKASQ
                     SLYDDIAKRGGRAVMWKTGHSLIKAKMKEEAAVLAGEMSGHMFFADRYFGYDDAVYAS
                     CRLVEILAKAQQPLSALVADLPRSVVTPEIRVDLPDTVKFDVVERIRRRFTEYVKTKQ
                     SLGPRKLMLKNLITIDGVRAVFDEGWGLIRASNTQPALVLRFEATSSAQLNVIRALIE
                     DELAVAKRALGC"
BASE COUNT          664 a          902 c          740 g          701 t
ORIGIN      
        1 accgaagcgc atccttcacg ctgcccgtga ccgacggctg aggatctttg ttgtcatgca
       61 gcacgtcgtg attgttgagc ttgagaatga ggggaatctg acccgcaaac tcacttgccc
      121 cggcttccag aaatcccagc ggtgcggcat aggcgttaca ccccgcctcg atcgcgagct
      181 ggaaatggta gtgaggattg tacccgggtg ggttcgccga aaaactccgt gccggaccat
      241 gctcgaaccc ttgatccacc ggtaaaatga ccaacttgcc ggtcccggcc aatttgccgg
      301 accgaagcaa acgagcgatg ttggttttag tcccggcatt gtcgctgcca taccaactca
      361 gaatttcttt aacccggtct cccataattc cctccacaca acatcacagt accacataaa
      421 tgtgcaactt aggctttccc ctgaacgtgc gcttcagctt gctcgacatc ttgtcggctt
      481 ccgatgatca acggcacgcg ctggtgcaat ttcttgggct ccacttccaa cactctcgat
      541 gtccccgtcg aggcccttcc gccggcctgc tcgacgacaa acgccaacgg attcgcctcg
      601 tagagcaacc gcagctttcc ttctggttta ccggcttcac ccggatagag atagatccct
      661 cccccgagca acagacgatg cacgtcggcg accagacagc ccgaatacct gctgctgtac
      721 ggacggcctg tcgtcttgtc gctgactttg agtgaatcca aatatttttt cgttccctcc
      781 gaccacttgt ggtagttccc ttcgttgaca gcgtagaccc tccccttttc gggaatcctg
      841 atcttctcgt gcgataggag atattctccg atgtcgggtt cgagcgtgaa tccatacaca
      901 ccttgtccta cggtatacac caacatggta ctggatccgt acaagagata cccggccgcg
      961 acttgttcgg tccctcggcc taacaattct tcctcagtgg gtaaacgatc ggtcctgcca
     1021 tacttgagaa cagaaaaaat ggccccgagc ggcatattgt tgtccgtgtt ggaggagcca
     1081 tcgagcggat cgaacaacaa catgtacttg ccgtgcggcc aattccccgg gatcgagatc
     1141 ggtttttcca tttcttccga ggccaaggcg cagacatacc cactgtgttg aaagactttg
     1201 acgaagtcgt cgttggcgat ggcgtccagc ttcttgaccg tttctccttg cacattcgtg
     1261 tcaccggtcg ttccaagaat gttgatcagt ccggcacgcc gaagatcttg cgaaatgagc
     1321 ttgccgacca gaccaatctg ggtcaacaaa ctggagaatt cttccgtcgc atcttgatgc
     1381 gaggtctgat tctgaatgat aaatcggcct aaggtaaggg gaaattctcg catactgacg
     1441 ctcagtataa cgggtttgac ccgtgcgaac aatcatcaat gttcaatact tttgctcggc
     1501 ggtgccgctt tcctcaaagc catcgaccag gtctgcgacg atcgatccga gcacgacctt
     1561 cgctgtcatc cgaacagggg tctttcgttc atcattcgtg acccacacgc ggatattccc
     1621 cttattcatg aagaggccat gaaacggcat gatcacgagc actcggacag tctccgccct
     1681 tccccatgcc ccctcaatgg tctcgatagc ctcgacacga acctcgaccg gacgattttt
     1741 cttgtcatga tacacattca ttttcaacga ggcacccgga ttcggcggca atcgcgtacg
     1801 cgtatagtac aagcatgaaa tgatatcctg tgtcccgacc gggataggga gcgactccgt
     1861 cgttcctcct ctgacggccg ttaccacgcc ctctttttga tgaaagacat actcgatatc
     1921 ttctttcttt ttgccttcgc gccgacggaa cgtcatatgt tccggagtca gagtatccaa
     1981 gtccagttcc gattccactc gattgtcgac agggaaaaac ttggtgataa ccggtgttga
     2041 ttgtgcggtc cccaccagct tcgcgacggc ctgatccttt cgcccatcta ttcgggccac
     2101 ctccatcacc gcgatgactg cggtcaggtt gagccaggag acctcatatg tcagccgttc
     2161 tccaacctga aacggacgct tcgtttcgcc gagaggctca gcttcaatgg agccggcaga
     2221 gtatgatgac agagcaacca cagcgacaag gacgacatac cggcacgtct gagcgacact
     2281 gggagggcga agaaacctgg tagggacagg tgtcaacacc cgagcgcccg tttcgccacc
     2341 gcgagttcat cctcgataag cgctcgaatg acgttgagtt gtgcggagga tgtcgcctca
     2401 aatctcagta ctaaggccgg ctgggtgttg gaagctctga taagccccca cccttcatcg
     2461 aatacggcac ggacgccgtc gatcgtgatc aggtttttga gcatcagttt cctcggcccg
     2521 aggctctgtt tggtcttcac gtactcggtg aatctccggc gaatccgctc aacgacatcg
     2581 aacttaacgg tatcaggaag atccactcgt atctcgggtg tgacgaccga ccgaggcaga
     2641 tcagccacta aagccgaaag cggctgttga gccttcgcca aaatttctac gagccggcac
     2701 gacgcataga cggcgtcgtc gtacccaaaa taccgatccg cgaaaaacat gtgcccggac
     2761 atttcaccgg ccaataccgc cgcctcctcc ttcattttcg ccttgataag cgaatgaccg
     2821 gtcttccaca tgacggcgcg cccaccgcgc ttggcaatgt catcatagag actctgtgac
     2881 gccttcactt ccgagatgat ggtgctgccc ggtttcatcg ctaagatgtc acgtgagtag
     2941 aggaccaaca ggcggtcccc ccacagcaca tgaccctgct catcaacgac tccgattcgg
     3001 tccgcat
//
LOCUS       DATMTI010000319         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_563357, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000319 DATMTI010000000
VERSION     DATMTI010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563357"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..>1663)
                     /locus_tag="VN039_05325"
     CDS             complement(56..>1663)
                     /locus_tag="VN039_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWV45444.1"
                     /translation="HVPRYMAFWEFSLSNRRLRYAFPPHNSFPTGSVRPAAMAERSVM
                     VMNERPDTVIPASATSPPETLNRSLSLWNSLTIGFATVSPVAGLYAIIGVQTVVAGGG
                     WFPALALCLVMQLLVATVYAELSSQIPIAGGAYKWARQLGGSTTGTYAGAIYVSSTIA
                     MLTTTAYTGGVWLAIFFGSGSGTGVTLVIWGAVFMLICTALNLVHVAVFKFLISLGVY
                     AEIVGSFGVALLLFFFFRQHDFSELFAHLGTGTAPSQTAAFLAALAISGWAFIGFDAC
                     STIAEETHEPKRMVPRAIFFSLCMVGSVVLFNSAALTLSFNRDTLVHTSAASDPVTPV
                     IVHNFGAWAETPFLTIVMIAFLACGSSMVQYTSRIVFSMAREGSMPAVLSRVTAAGAP
                     HNAVLFTVLLATLGLLFGLNDEAVATVLAFGTGGLYAMFAMTTGVGLYTRLTGRWDPS
                     LGQLKLGAWGLLINTAAFLWSVFEFVNIAWPRPYATSPDAPWWQLWAVPLVLGSILGV
                     TTLYLLIVKPTRDDLLMRPRASAPTQE"
BASE COUNT          419 a          466 c          489 g          289 t
ORIGIN      
        1 gactcggttc gaactccctg gttgcaacac gaaactcagc gaactagccg gcttttcatt
       61 cttgagttgg tgcggacgcc ctcggacgca tcaataagtc gtctcttgtg ggtttaacga
      121 tgagaagata aagcgtggtc acccctaaga tgcttccgag aaccagcggg acggcccaga
      181 gttgccacca aggtgcgtcg ggtgaagttg cgtagggacg tggccacgca atattgacga
      241 attcaaacac cgaccacaga aacgccgccg tattgatcag taagccccaa gcccccagct
      301 tcagttgacc taacgagggg tcccatcgac cggtgagccg ggtgtagagt cccacaccgg
      361 tggtcatcgc aaacatggca tacaacccgc ccgtgccgaa cgccagcacc gtggcgaccg
      421 cttcatcatt gagcccgaag agcaatccga gcgtggccag cagaaccgtg aacagcacgg
      481 cattatgcgg agcacccgct gcagtgaccc ggctgagaac ggccggcata cttccttcgc
      541 gagccatgga aaacacgata cgggacgtgt attggaccat ggatgatcca caagccagga
      601 aggcgatcat gacaatcgtc agaaacgggg tttccgccca ggcgccgaag ttgtggacga
      661 tcaccggtgt gacgggatcg gacgcggcgc tcgtatggac cagggtgtcg cggttgaaac
      721 tcagggtgag ggcggcggag ttgaagagca ccacgcttcc caccatgcag agagagaaaa
      781 agatcgcgcg cggcaccatc cgtttaggct cgtgagtttc ttcggcaatg gtcgaacagg
      841 catcgaagcc gatgaacgcc caaccggaaa tcgcaagagc cgccagaaac gcagcggtct
      901 gactgggagc ggttcctgtc cctagatgtg caaacagttc ggagaagtca tgctgtcgaa
      961 aaaaaaagaa caacagcagc gcgactccga atgagccgac gatttccgca tagaccccca
     1021 gggaaataag aaacttaaat accgcgacat ggaccagatt gagggcggtg cagatcagca
     1081 taaacaccgc cccccagatt acaagcgtga ctcctgttcc actacctgac ccgaaaaaga
     1141 tcgcgagcca aaccccgcct gtgtaggcgg tcgtggtgag catggcgatc gtggagctga
     1201 catagatggc tccggcatag gtgccggtgg tggagcctcc gagctgtctc gcccatttgt
     1261 acgcgcctcc cgcgatcgga atctgcgagg acagctccgc atacacggtc gccaccagca
     1321 gctgcatcac cagacagagc gccagcgctg ggaaccaacc accgcctgcg actacagtct
     1381 ggacaccgat aatggcgtac aatccggcaa ccggtgagac ggttgcgaat ccgatggtga
     1441 ggctgttcca gagactgagg ctgcggttga gtgtctcggg aggacttgta gcggaagcag
     1501 gaataaccgt atccggacgt tcgttcatga ccatgacaga cctctccgcc atggcagcag
     1561 gtctgactga tccggtaggg aaagaattat ggggtggaaa tgcataacga agcctcctgt
     1621 tggaaaggct aaattcccaa aaggccatgt acctcggtac atg
//
LOCUS       DATMTI010000320         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_96466, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000320 DATMTI010000000
VERSION     DATMTI010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96466"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VN039_05330"
     rRNA            <1..1355
                     /locus_tag="VN039_05330"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1536..1612
                     /locus_tag="VN039_05335"
     tRNA            1536..1612
                     /locus_tag="VN039_05335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1570..1572,aa:Ile,seq:gat)
     gene            1678..1753
                     /locus_tag="VN039_05340"
     tRNA            1678..1753
                     /locus_tag="VN039_05340"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1711..1713,aa:Ala,seq:tgc)
     gene            1943..>2433
                     /locus_tag="VN039_05345"
     rRNA            1943..>2433
                     /locus_tag="VN039_05345"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          576 a          590 c          771 g          496 t
ORIGIN      
        1 gtctgcggat cgagagggaa agcgataccg tgggtatcgc gctcttggat gggctcatgt
       61 cctatcagct tgttggtgag gtaatggctc accaaggctt cgacgggtag ctggtctgag
      121 aggacgatca gccacactgg cactgcgaca cgggccagac tcctacggga ggcagcagtg
      181 aggaatattg cgcaatgggc gaaagcctga cgcagcgacg ccgcgtgggg gatgaaggtc
      241 ttcggattgt aaaccccttt cggcagggaa gatggagcgg gcaaccgttc ggacggtacc
      301 tgcagaagca gccacggcta acttcgtgcc agcagccgcg gtaatacgaa ggtggcaagc
      361 gttgttcgga tttactgggc gtacagggag cgtaggcggt tgggtaagcc cttcgtgaaa
      421 tctccgggcc taacccggaa agtgcggagg ggactgctcg gctagaggat gggagaggag
      481 cgcggaattc ccggtgtagc ggtgaaatgc gtagagatcg ggaggaaggc cggtggcgaa
      541 ggcggcgctc tggaacattt ctgacgctga ggctcgaaag cgtggggagc aaacaggatt
      601 agataccctg gtagtccacg ccttaaacga tggatactaa gtgtcggcgg gttaccgccg
      661 gtgccgcagc taacgcagta agtatcccgc ctgggaagta cggccgcaag gttgaaactc
      721 aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc gacgcaacgc
      781 gaagaacctt acccaggctg gacatgcagg tagtaggagg gtgaaagccc aacgaggtag
      841 caataccagc ctgctcaggt gctgcatggc tgtcgtcagc tcgtgccgtg aggtgttggg
      901 ttaagtcccg caacgagcgc aacccttgtc ttcagttacc aacgggtcat gccgggaact
      961 ctggagagac tgcccaggag aacggggagg aaggtgggga tgacgtcaag tcagcatggc
     1021 ctttatgcct ggggccacac acgtgctaca atggccggta caaagcgctg caaacccgta
     1081 agggggagcc aatcgcaaaa aaccggcctc agttcagatt gaggtctgca actcgacctc
     1141 atgaaggcgg aatcgctagt aatcccggat cagcacgccg gggtgaatac gttcccgggc
     1201 cttgtacaca ccgcccgtca caccacgaaa gtttgttgta cctgaagtcg ttggcgccaa
     1261 ccgcaaggag gcagacgccc acggtatgac cgatgattgg ggtgaagtcg taacaaggta
     1321 gccgtagggg aacctgcggc tggatcacct cctttctaag gagtcacatg actctacgga
     1381 aaactcgggc tgagggccac ctcttcaata catgcttaca cgcttacact atctggttgt
     1441 tgtgagggtt caggcggcgc tcctacataa aggttgattt atattttatg gggagatcga
     1501 gtcgaatacg ggccctggtc gcttcagact cagctgggcc tgtagctcag ttggttagag
     1561 cacgcgcttg ataagcgcgg ggtcgatagt tcaactctat ccaggcccac catctgagtc
     1621 ctgatcctct aggcgctagg cgcatggtat caggagcaga ctctttctgt tgagaatggg
     1681 gctgtagctc agttgggaga gcacctgctt tgcaagcagg gggtcgtcgg ttcaattccg
     1741 tccagctcca ccatgccaac ttcgcttgaa tcgtttgcca tccttaatcg gcttgtatgg
     1801 aagtttcagg caaacggatc gttagtttaa gttgtattgt tcaacatcct ctatgcagtt
     1861 cttggcgcaa gggcgccaag gttgttcttt gacaactgaa taggtcttga tgaaagacca
     1921 tgtgtatcag gagcaaagtg attgttaagc tactaagggt gtacggtgaa tgccctggca
     1981 tcagcaggcg atgaaggacg tagctagctg cgataagccg cgggaagccg caagcaggcc
     2041 gtgatccgcg gatgtccgaa tggggaaacc caggcgatga atgccgccta tccgcgtctg
     2101 aatccatagg acgcgagaag cccacgcagg gaagtgaaac atctcagtac ctgcaggaat
     2161 agaaatcaat cgagattccc ccagtagtgg cgagcgaaaa gggaatagcc taaaccacag
     2221 tgatgtcaag cccgtgtgcg ttgtcgctgt ggtgtcgtag ggcttcgttt gacggcgata
     2281 cggcgccgtc ggcaagtcaa aaattaggat cttagtcaaa cggtctggaa aggccggcca
     2341 aagaaggtga cagccctgta ggcgacaaga tcctaacttg ctggacggag ttcctgagta
     2401 ccacggggct cgagcaaccc tgtgggaatc cgg
//
LOCUS       DATMTI010000321         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_12029, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000321 DATMTI010000000
VERSION     DATMTI010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12029"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..898
                     /locus_tag="VN039_05350"
     CDS             269..898
                     /locus_tag="VN039_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWV45445.1"
                     /translation="MPFSTAKFLKFRSGMQKRIGSLRSKTEDSLECAVETMMEERVDG
                     FFLVEQGLEEIIKSLVEIEEELEAIRDLTGAMRLESRLEFVEERWDEFDSEIRERPRR
                     RRKRVSLADMLKAAGGTGQLSENPHAVNNAVDAYAIMGVEFGSSLADVTAAFRTKAKQ
                     LHPDANNGDRTSEPELRRMLEAYQFLKEYLSLSNTEPMPPPDRPFSPSE"
     gene            912..2117
                     /gene="rnd"
                     /locus_tag="VN039_05355"
     CDS             912..2117
                     /gene="rnd"
                     /locus_tag="VN039_05355"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HWV45446.1"
                     /translation="MPIPYQYITSATALSELCEQLRDSPRLALDTEFVGEESFVPKLE
                     LIQVATERTAAIIDFPAVLSGGTLDGFWELVCDSRIQKIVHAGRQDLDLFAVHAGRIP
                     TPFFDTQIAAAMVGLGPQIAYANLVQRVHGKRLDKAHTFTNWSARPLSQDQLAYALED
                     VTFLLAIHDHLHTKLSKLGRLQWVHEEFSRLESVVGETRREPQERYQRIRGWDQLKPK
                     SAAVLRELAAWREGEAKRRNVPRSRVVRDEVLLQLARHPLRHQDELRKVRGLHASEID
                     RNGESILAVIQTALALPPSAWPALAKERKPEPESNGFVELLQAIVKACALEKEIAPTL
                     LATTADLQELVDAKTNRSTPDLPVLKGWRRTLVGELLLDALDGKVAFTVDQQTRTIRW
                     SRSEPTTDR"
BASE COUNT          479 a          589 c          651 g          441 t
ORIGIN      
        1 catcgcccat gtgattccgg agaatacgtt aagcgaggac tacatcatcc ccagcgtgtt
       61 tgacaaggaa gtggtcccgc gtgtcgcgcg ggcagtcgct gccgctgcac gcgccagcgg
      121 agtggccaga aggcgggcca agccggacga tccctccact tttgagtaat caccataagt
      181 ctccaatggg aaataaaccg tcggttgcag ccttcaggct gtcgtcgtgg ctgtgataca
      241 ataccctttt tgaaagtccg agggagccat gccgttctcg accgcgaaat ttctgaaatt
      301 ccgaagcggg atgcagaagc gtatcggttc actgcgttca aagacggaag acagcctgga
      361 atgtgccgtc gagacgatga tggaggaacg tgtcgacggg tttttcctgg tggaacaggg
      421 actcgaggaa atcatcaagt ccctcgtcga aatcgaggaa gaactggagg cgattcgaga
      481 tttaaccggg gccatgcgct tggagtcccg gctggagttc gtcgaggagc gctgggatga
      541 attcgacagc gaaattcgtg aacgtccccg acgtcgccgc aaacgagtca gtctcgccga
      601 tatgctcaaa gccgccggtg gaacgggaca gctgtcggag aatccccatg ccgtgaataa
      661 cgcggtggat gcctatgcga tcatgggtgt ggagttcggc agctctctcg ccgatgtcac
      721 cgccgcgttt cgaaccaagg cgaaacagct ccatcccgac gcgaacaacg gcgatcgcac
      781 ctccgaaccg gaactccgcc gcatgttgga agcgtatcag tttctgaagg aatacctcag
      841 cctcagcaat acggagccca tgcctcctcc ggatcgtccc ttcagcccgt ccgagtgatg
      901 caccggtgac gatgcccatc ccataccaat acatcacgag tgcaactgca ctcagcgaat
      961 tgtgtgagca actgcgggat agcccgcgcc tggcgctcga cacggaattc gtgggcgagg
     1021 agagttttgt gccgaagctc gaactcattc aagtcgccac agaacgcact gcggctatta
     1081 tcgactttcc agccgttctg tcagggggaa cccttgatgg gttctgggag cttgtctgtg
     1141 attcgaggat ccaaaagatc gtgcatgcag gacgtcaaga tctggacctc tttgcagtcc
     1201 acgccgggcg aatcccaacg ccattcttcg acacccagat tgccgcagct atggtcggct
     1261 tgggtcctca gattgcctat gccaaccttg ttcaacgggt gcatgggaag aggctggaca
     1321 aggcgcatac gtttacgaat tggagcgccc gcccgctgtc ccaagatcag ttggcctatg
     1381 cgctggagga cgtgaccttt ctattggcga ttcacgatca tctgcatacc aagttgtcga
     1441 aactcggccg gttgcaatgg gtgcacgagg agttttctcg tcttgaaagt gtcgtgggcg
     1501 agactcgtcg agaacctcaa gaacggtacc aacgtatacg aggatgggat cagttgaagc
     1561 cgaaatcggc cgcggtgctt cgggaactcg cagcctggcg tgaaggggaa gcgaagcgga
     1621 gaaatgtgcc tcggagccgg gtcgtccggg atgaggtgct gcttcagctg gcgcggcatc
     1681 ctctacggca ccaggatgag ttgcgcaaag tcagaggtct ccatgcttca gaaatcgacc
     1741 gcaatggaga gtcgattctt gctgtgattc aaacggcgct tgcgcttcca ccctcggctt
     1801 ggcctgcgct cgccaaagag cgaaagcctg agccggagtc gaatggattt gtggagctgc
     1861 ttcaggcgat tgtcaaggcc tgtgccctgg agaaagagat tgcgccgacc ctcttggcga
     1921 ccacggccga cctccaagaa ttggtcgatg ccaaaacgaa tcgatcgacg ccggatctcc
     1981 cagtcctgaa gggatggagg cgaaccttgg tgggtgagct tctgcttgac gcgctggacg
     2041 gcaaggtcgc ctttaccgtt gatcaacaga ccaggacgat tcggtggtcg cgctccgagc
     2101 cgaccaccga tcgctgagcg attaggcgtt aatcgaagtg gccgcaggat gttcaaaaag
//
LOCUS       DATMTI010000322         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_733129, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000322 DATMTI010000000
VERSION     DATMTI010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733129"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VN039_05360"
     rRNA            complement(<1..1536)
                     /locus_tag="VN039_05360"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          312 a          492 c          354 g          441 t
ORIGIN      
        1 atcgaacccc atagccatag gaggtcctgc ttatccttct gcgtcacccc atcgtaataa
       61 cgaggtatcg ggaggtccgg aatattaacc ggatttccat cgtctacgcc tctcggcctc
      121 gacttaggga ccgcctaacc ctgagcggat taaccttcct caggaaacct tagactttcg
      181 gtgcggaggg ttctcacctc cgttatcgct acttatgccg gcagggtctc ttcgcaacgc
      241 tccatcggtc ttctcagtcc gacttcactg ctgttgagaa tgctctccta ccacgcgcac
      301 cactgaaggt acgcatccgc tgcttcggta tacagtttta gccccgttgt attgtcggca
      361 ccggaccgct tgaccagtga gctattacgc tttctttaaa ggatggctgc ttctaagcca
      421 acctcctggc tgtctgagcg ttccgacttc ctttcccact taactgtaat tttgggacct
      481 tagcaggcgg tctggactgt tttcctctcg actgtgaagc ttagccccca cagtctgact
      541 gccaggctgg ttgtgatcgg cattcgaagt ttgattggat tcagtaagcc ggaaagcccc
      601 ctagtccatc catgtctcta ccaccaatcc aaatcacccg acgctagccc taaagctatt
      661 tcggagagaa cgagctatca cggaatttga ttagcctttc acccctaccc tcagctcatc
      721 cgagcttttt tcaacaaaca ccggttcggt cctccaatgg gtgttacccc atcttcaacc
      781 tggccaaggg tagatcatcc cgcttcgcgt ctattcctgc caacttatcg ccctattaag
      841 actcgctttc gctgcggctc gctcacgctt aaccttgctg acaagaataa ctagccggct
      901 cattatgcaa taggcacgcg gtcaggcatt tccttacgga catagccctc ccactgcttg
      961 taggcacacg gtttcaggta ctttgcactc ccctaacagg ggttcttttc gcctttccct
     1021 cacggtactg gttcactatc ggtcagaaac gagtatttag ccttgcggaa tggtctccgc
     1081 ggattcaagc agggtttcac gtgccccgcc ttactcaggt gcctgcttcg agtctggatc
     1141 gctttcgcct acgcgcctgt cacgctctat ggaccccctt tccagaggat tcggctaacg
     1201 accagattgg taactctact gttgcaggtc ctacaacccc cgccgcaccg aaatgcaacg
     1261 ggtttgggct gttccgattt cgctcgccac tactttcgga atcgaggttt ctttcttttc
     1321 cttcaggtac tgagatggtt cacttccctg agttcgcttg gtcccgccta tgtattcagc
     1381 gggtcattcc tgaggtttgc tcaggagggt ttccccattc ggaaatctcc ggatatagcg
     1441 cctatgtgcg gctccccgaa gcttatcgca gcttgtcacg tccttcatcg cctgtttctg
     1501 ccaaggcatc caccgtgcgc ccttagtagc ttgaccataa aattaactcc acgcaggagc
     1561 cactctatgt tttgccgcct tatgcgaact aacttgcaa
//
LOCUS       DATMTI010000323         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_139217, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000323 DATMTI010000000
VERSION     DATMTI010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139217"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2755)
                     /locus_tag="VN039_05365"
     rRNA            complement(<1..2755)
                     /locus_tag="VN039_05365"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3073..>4269)
                     /locus_tag="VN039_05370"
     rRNA            complement(3073..>4269)
                     /locus_tag="VN039_05370"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          848 a         1450 c         1007 g          964 t
ORIGIN      
        1 actccagccc caggatgcga cgagccgaca tcgaggtgcc aaaccatccc gtcgatatgg
       61 actcttgggg aagatcagcc tgttatcccc ggggtacctt ttatccgttg agcgacggcg
      121 cttccacaag ccaccgccgg atcactagtc ccgactttcg tccctgctcg acccgtcggt
      181 ctcacagtca agctcccttg tgcacttaca ctcaacacct gattgccaac caggctgagg
      241 gaacctttgg gcgcctccgt tactctttag gaggcaaccg ccccagttaa actacccatc
      301 agacactgtc cctgatccgg atcacggacc caggttagac atccagcacg accagagtgg
      361 tatttcaacg acgactccac ccaaactggc gtttgagctt caaagtctcc cacctatcct
      421 acacaagccg aaccgaacac caatatcaag ctatagtaaa ggtcccgggg tctttccgtc
      481 ctgctgcgcg aaacgagcat ctttactcgt agtgcaattt caccgggcct atggttgaga
      541 cagtcgagaa gtcgttacgc cattcgtgca ggtcggaact tacccgacaa ggaatttcgc
      601 taccttagga tggttatagt taccaccgcc gtttactggc gcttaagttc tcagcttcgc
      661 caccccgaag aatgactaac cggtcccctt aacgttccag caccgggcag gcgtcagtcc
      721 gtatacatcg ccttacggct tcgcacggac ctgtgttttt agtaaacagt cgcttctcgc
      781 tggtctctgc ggccaccccc agctcaccga gtaaatcgga tcaccgagtg tggcccccct
      841 tctcccgaag ttacgggggc attttgccga gttccttaac catagttcac ccgaacgcct
      901 cggtattctc tacctgacca cctgagtcgg tttagggtac gggccgccat gaaactcgct
      961 agaggctttt ctcgacagca taggatcatc cacttcgcca caatcggctc ggcatcaggt
     1021 ctcagactat atggtgtgcg gatttgccta cacaccgtcc tacaccctta ccccgggaca
     1081 accaccgccc gggatggact accttcctgc gtcaccccat cactcaccta ctacaagtct
     1141 ggtccgtcgg ctccaccact ttccattccc cgaagggtcc ggaacggctt cacggactta
     1201 gcatcgcctg gttcgatgtt tgacgcttca cagcgggtac cggaatatca accggttatc
     1261 catcgactac gcctgtcggc ctcgccttag gtcccgactt accctgggca gatcagcttg
     1321 acccaggaac ccttagtcaa tcggcgcaca cgtttctcac gtgtgtatcg ctactcatgc
     1381 ctgcattctc actcgtgaac cgtccacaac taccttccgg tgctgcttca cccggcacac
     1441 gacgctcccc tacccatcaa cacacccgtt ggggcttctt gtgtcaatga cacgacttcg
     1501 gcggtacgct tgagccccgc tacattgtcg gcgcggaatc actagaccag tgagctatta
     1561 cgcactcttt caagggtggc tgcttctaag ccaacctcct ggttgtctct gcgactccac
     1621 atcctttccc acttagcgta cgcttagggg ccttagtcga tgctctgggc tgtttccctc
     1681 tcgaccatgg agcttatccc ccacagtctc actgccgtgc tctcacttac cggcattcgg
     1741 agtttggcta aggtcagtaa cccggtaggg cccatcgcct atccagtgct ctacctccgg
     1801 caagaaacac acgacgctgc acctaaatgc atttcgggga gaaccagcta tcacggagtt
     1861 tgattggcct ttcaccccta accacaggtc atcccccagg ttttcaaccc tggtgggttc
     1921 ggtcctccac gaagtcttac ctccgcttca acctgcccat ggctagatca ctccgcttcg
     1981 ggtcttgagc gtgctactga aacgccctat tcggactcgc tttcgctacg gcttccccac
     2041 ccgggttaac ctcgcaacac accgcaaact cgcaggctca ttcttcaaaa ggcacgcagt
     2101 cacgagaatg gagcaagctc cactccgacg ctcccacggc ttgtaggcac acggtttcag
     2161 gtactatttc aatccgctcc cgcggtactt ttcaccattc cctcacggta ctatccgcta
     2221 tcggtcacca gggaatattt aggcttagcg ggtggtcccg ccagattcac acgggatttc
     2281 tcgggccccg tgctacttgg gtgtctctca aacgagccgt tgatgtttcg actacggggg
     2341 tcttaccctc tacgccggac ctttcgcatg tccttcgcct acatcaacgg tttctgactc
     2401 gcctcacagc cggcagactg tgaaagagag atcccacaac ccccacgacg caacccctgc
     2461 cgggtctcac acgtcgtagg tttggcctca tccggtttcg ctcgccacta ctcccggaat
     2521 cacggttgtt ttctcttcct gcgggtactg agatgtttca cttccccgcg ttccctccac
     2581 actgcctatg tgttcagcag cgggtgacag cccatgacga ctgccgggtt tccccattcg
     2641 gaaacccccg gatcaaagcc tggttgacga ctccccgggg actatcgtgg cctcccacgt
     2701 ccttcatcgg ttcctggtgc caaggcatcc accgtgcgcc cttaaaaact tggccacaga
     2761 tgctcgcgtc cactgtgcag ttctcaaaca acgaccagcc acccatcacc cccaaccctg
     2821 acggctggag ttcactgggg ccggcatcag aaggacgagc aaacgctcgc accctcagac
     2881 acccaacagc gtgcccgacc cgatcccgcc cggagatcat gctttccacg ccccgaagag
     2941 cagtactcgc aagcctccga cccgagactc tggccgaata atcaacgttc cacccatgag
     3001 caaccagcac cggacgttcg ccgatgaact ggcccctgga cagccttgcg gctgcctaga
     3061 agtgctcctt agaaaggagg tgatccagcc gcaccttccg gtacggctac cttgttacga
     3121 cttcgtccca atcgccagtc ccaccttcga cagctccctc ccacaagggg ttgggccacc
     3181 ggcttcgggt gttaccgact ttcgtgacgt gacgggcggt gtgtacaagg cccgggaacg
     3241 tattcaccgc agcaatgctg atctgcgatt actagcaact ccgacttcat ggggtcgagt
     3301 tgcagacccc aatccgaact gagaccggct ttttgagatt cgctccacct cacggtatcg
     3361 cagctcattg taccggccat tgtagcacgt gtgcagccca agacataagg ggcatgatga
     3421 cttgacgtcg tccccacctt cctccgagtt gaccccggcg gtctcctgtg agtccccagc
     3481 accacaaggg cctgctggca acacaggaca ggggttgcgc tcgttgcggg acttaaccca
     3541 acatctcacg acacgagctg acgacagcca tgcaccacct gtacaccgac cacaaggggg
     3601 cgaccatctc tggccgtttc cggtgtatgt caagccttgg taaggttctt cgcgttgcgt
     3661 cgaattaagc cacatgctcc gccgcttgtg cgggcccccg tcaattcctt tgagttttag
     3721 ccttgcggcc gtactcccca ggcggggaac ttaatgcgtt agctgcggca cggacaacgt
     3781 ggaatgtcac ccacacctag ttcccaacgt ttacggcgtg gactaccagg gtatctaatc
     3841 ctgttcgctc cccacgcttt cgctcctcag cgtcagtaat ggcccagaga tccgccttcg
     3901 ccaccggtgt tcctcctgat atctgcgcat ttcaccgcta caccaggaat tccgatctcc
     3961 cctaccacac tctagtctgc ccgtatcgaa tgcagacccg gggttaagcc ccgggctttc
     4021 agagcggact tactgagccg cctacacgcg ctttacgccc aatgattccg gacaacgctt
     4081 gccccctacg tattaccgcg gctgctggca cgtagttagc cggggcttct tccaccggta
     4141 ccgtcttcta ctcgtcccgg tcgaaagggg tttacgctcc gagaagcttc atcccccacg
     4201 cggcgtcgct gcgtcaggct ttcgcccatt gcgcaagatt ccccactgct gcctcccgta
     4261 ggagtctgg
//
LOCUS       DATMTI010000324         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_818743, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000324 DATMTI010000000
VERSION     DATMTI010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818743"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VN039_05375"
     rRNA            complement(<1..996)
                     /locus_tag="VN039_05375"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1267..>1709)
                     /locus_tag="VN039_05380"
     rRNA            complement(1267..>1709)
                     /locus_tag="VN039_05380"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          309 a          540 c          407 g          453 t
ORIGIN      
        1 gtctgactcc cggatatgaa tgaatggcat tcggagttta tctgaattcg gtaacccgag
       61 atgggcccct agtccaaaca gtgctctacc tccatcattc tcaattccga ggctagccct
      121 aaagctattt cggagagaac cagctatctc caagttcgat tggaatttct ccgctaccca
      181 cacctcatcc ccgcactttt caacgtgcgt gggttcgggc ctccatccag tgttacctgg
      241 acttcaccct ggacatgggt agatcacctg gtttcgggtc tacgaccaca tactcattcg
      301 ccctattcag actcgctttc gctgcggctc cgtctcttca acttaacctt gcatgtaatc
      361 gtaactcgcc ggttcattct acaaaaggca cgctatcacc cattaacggg ctctaactac
      421 ttgtaggcac acggtttcag gatctttttc actccccttc cggggtgctt ttcacctttc
      481 cctcacggta ctggttcact atcggtcact agggagtatt tagccttggg agatggtcct
      541 ccctgcttcc gacgggattt cccgtgtccc gccgtactca ggatccgctc gggagggaac
      601 gaagtttcga ctacagggct gtcaccttct ctggccggcc gttccagacc ggttcgtcta
      661 ccccgttcct ttctcactcc cacaatgagc ggtcctacaa ccccaagagg cacgcctctt
      721 ggtttgggct gttcccgttt cgctcgccgc tactcaggga atcgcgtttg ctttcttctc
      781 ctccgggtac taagatgttt cagttccccg ggtgtgccct ccatgcccta tggattcagg
      841 catggatact gccccattac ggacagtggg ttcccccatt cggacatctc cggatcaacg
      901 cttgcttaca gctccccgga gcgtttcggc gtttgccccg tccttcatcg gctcctagtg
      961 ccaaggcatc caccgtgcgc cctttctagc ttaacctaca gcgctctcgg cttcttcctt
     1021 attgtctagc gtcggctcgc cgccgctcgg ggtcaaataa ccttcgcctt ccgggtgcaa
     1081 gcacccttgc aggcgaagaa catttgccca tcgcggcgaa acgctcgcct ccgcttttcc
     1141 ggttatctag ttttcaagga acgatttttg tcattgagag aacatgactc gctccctcaa
     1201 aactgaacga aacagaagcg ctgtgtatcg acagttggcc attgcggcct tcgcttttcc
     1261 ccttagaaag gaggtgatcc agccgcacct tccggtacgg ctaccttgtt acgacttcac
     1321 cccaatcact tgccccacct tcggcggctg gctcccttgc gggttacctc accgacttcg
     1381 ggtgttgcaa gctctcgtgg tgtgacgggc ggtgtgtaca aggcccggga acgtattcac
     1441 cgcggcatgc tgatccgcga ttactagcga ttccggcttc atgcaggcga gttgcagcct
     1501 gcaatccgaa ctgagagcgg ctttttggga ttcgctcccc ctcgcgggtt cgcagccctt
     1561 tgtaccgccc attgtagcac gtgtgtagcc caggtcataa ggggcatgat gatttgacgt
     1621 catccccacc ttcctccggt ttgtcaccgg cagtcacctt agagtgccca actgaatgct
     1681 ggcaactaag atcaagggtt gcgctcgtt
//
LOCUS       DATMTI010000325         4672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_182187, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000325 DATMTI010000000
VERSION     DATMTI010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182187"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VN039_05385"
     CDS             <1..1002
                     /locus_tag="VN039_05385"
                     /inference="COORDINATES: protein motif:HMM:NF023081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HWV45447.1"
                     /translation="TPQDTTEQKQDLTVATQKHVPMQFLSSELTLSLDDFSDRIIEPA
                     MMVLAANAESDCLTMAKDVYNQTGTPGTTPNTALPYLTAHAIVSGGLAPIGKRCYHIR
                     PLDNATLVDALKALQQSAPDIAEQYREGIMGRAFGGDWYENTLMPRITVGNKVASVVV
                     SGGSQTGSSLTIGGVSAADTFLKGQVFTIQGVNSVHPESRADTGQLQKFVVTADTTSA
                     GSTVAIPISPSIVVTGALQTVNGSPANNAPITFDGTANLQYGMNLHFYKTAFAVVFAD
                     LDMPKGLDFAHREQVDGISLRVLRQFEARTDLWVTRADILYGYKTIRPEFACRIATS"
     gene            1060..1248
                     /locus_tag="VN039_05390"
     CDS             1060..1248
                     /locus_tag="VN039_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45448.1"
                     /translation="MNHTYNPDTPYATLMRGRPDEPDSHQHIAVYSKADYEAFRKKGW
                     LKPVEFMERVDRARRKDG"
     gene            1251..1415
                     /locus_tag="VN039_05395"
     CDS             1251..1415
                     /locus_tag="VN039_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45449.1"
                     /translation="MDFVEFPKWMFKGNQDKPDTLLVADQAAQDKAAKKGWKTHDLFY
                     VEQLIEESTK"
     gene            1434..2210
                     /locus_tag="VN039_05400"
     CDS             1434..2210
                     /locus_tag="VN039_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45450.1"
                     /translation="MSVGVTSPNLQGPKRVKDLIQRAYRIAHVLGKGEALSLVTASDG
                     FESLNDLIEQAMIEKTFATYQTEIPIPLQAQKILYTVGPSTASPPPDITSTRPAEVLS
                     AYSRRSGRDLPLLVTHAKQDYDGVPNKNLASTGWSGLIYYQVAYPSGNVYVYPSPADD
                     LTVLYLTVLSVLTPYVSLDDEVSVPPGTYQYLKYALAKRVAADHSMPFGPENQEILER
                     CEAAFERNNIKAFPIASTGLNALSSGFSGYDIRSDTTRGY"
     gene            2210..3658
                     /locus_tag="VN039_05405"
     CDS             2210..3658
                     /locus_tag="VN039_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45451.1"
                     /translation="MGTHQAEPIFGMGNQQRSPFLSTVSRLNCIVEPTENGRQPAAIL
                     GLPGLVRYANTGQLPARGIFVHKGALTFYLVVGNLVIAILPNLTVHTIAMLTTDSGPV
                     WMDENGTQLFVNDAVTPIIYTYATGTAAIITDPDYPSGARGASFLQGRFWVYTVSGPN
                     AGRCYASDQYNGLSWDGLNFITPAARPTGITGIDRWADDLVIRGQGSIEWWSGAPSQI
                     SGALGFQPSSNANTSVGSIAERGSATVGDQYFFVGDRNGSTSVFEVVGYQIVPVSTPK
                     VNEDLSRLQASTAICTGYMVNEHPIFQVTIPGEITAGAVTWIYDSLTKQWSKRGSDGR
                     PYYRGLFAVGTNGDAYITDAFTGNLYRMHEQTYSEDGEPMPFEVGSTHLLKEGDGFAI
                     DKIQIDMETGLGNPMPPGNDPHGILYLSKDGGKTWPIERYVTLGKMGEFTTRAQEFQF
                     GLARDWAIRFRITEPIPRRVAGAYLVMRPGYA"
     gene            3655..3894
                     /locus_tag="VN039_05410"
     CDS             3655..3894
                     /locus_tag="VN039_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45452.1"
                     /translation="MILISNAPTNDPISNEQKNWVKWFSQLVQAFNSLTATGTTDQRP
                     NPAPFQGFMFFDTTLNKPIWAKTLTQYVDATGTNV"
     gene            3891..4229
                     /locus_tag="VN039_05415"
     CDS             3891..4229
                     /locus_tag="VN039_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45453.1"
                     /translation="MSYPLPMPTQTVSQSPIIRIVEAGGLYFRSVLIQEAGTIIPQHT
                     HDHDHVTLVGSGRVRGWQDGECLGDRGPGEVFEIPAGKGHVFQSLEPNTLLTCVHDVE
                     SALSVKRKGL"
     gene            4232..>4672
                     /locus_tag="VN039_05420"
     CDS             4232..>4672
                     /locus_tag="VN039_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45454.1"
                     /translation="MPMMAGAGMAAGGIGLGALGAMFGSQANKKQIDLMRQALDYQKG
                     IDTRTYNDLSPYRDFGKTQLNSLTDFLDTKDPSDYIDPGYQFRLNSGTNAINNDAADT
                     GMIQSGDTLRALTEYGQNMGSQEYNNAFNRYLGQGQFLQGLTGIG"
BASE COUNT         1026 a         1357 c         1339 g          950 t
ORIGIN      
        1 accccgcaag atacgaccga gcaaaagcag gatctcaccg tcgccacgca aaagcatgtc
       61 cccatgcagt tcctatcatc ggagctgact ctttcgctcg atgattttag cgaccgcatc
      121 attgaaccgg ccatgatggt cttggcggcc aatgccgaat cggactgtct gaccatggca
      181 aaggatgtct acaaccagac cggcacaccc ggcaccactc ctaacaccgc gctgccctat
      241 ctgaccgcac acgcgattgt aagcgggggt ttggcgccga tcgggaaacg gtgttaccac
      301 attcgccctt tggataacgc cacgctggta gatgccttga aggcgttgca gcagagcgct
      361 cctgatattg ccgagcagta ccgcgaaggc attatgggaa gggcattcgg cggcgattgg
      421 tacgagaata ccttaatgcc acggatcacc gtcggcaaca aggtcgcctc cgtcgtcgta
      481 tccggcggat cacagaccgg ttcctcgctg accatcggag gcgtctctgc cgccgatacg
      541 ttcctcaagg gccaggtctt caccattcag ggcgtcaact cggtccatcc tgaaagccgc
      601 gccgataccg gccagttgca aaagttcgtg gtcaccgcag acacgacttc tgccggatca
      661 actgttgcga ttccaatcag tccctcgatt gtcgttactg gggccttgca gacggtgaac
      721 ggttcaccgg ccaataacgc gcccatcacc tttgacggga cggccaatct gcaatatggc
      781 atgaatctcc atttctacaa gaccgccttt gcggtggtct ttgccgattt ggatatgccg
      841 aagggcctgg atttcgccca tcgggaacag gtggacggca tctcactccg cgtcttgcgg
      901 caattcgaag cgcgcactga cctgtgggtg acacgcgccg acattctgta cggatataaa
      961 acgatccggc cagagttcgc ctgtcgcatc gcaacttcgt aagtccgttg agggccgggg
     1021 gcagttccgc tcccggccta ttcggtttat ggaggcgcga tgaatcacac gtataaccct
     1081 gacacgccct atgcgacgct catgcgtggg agaccggacg aaccggactc tcatcagcat
     1141 atcgccgtct attcgaaagc agactatgag gcttttcgca agaaaggatg gctcaagcca
     1201 gtggaattca tggagcgggt cgatcgggcc cgcaggaagg atgggtgagc atggacttcg
     1261 tggaatttcc caaatggatg tttaagggca atcaggacaa gccggacaca ttactggtag
     1321 ccgatcaggc ggcccaggac aaggcggcca agaaaggctg gaagacccac gatctgttct
     1381 acgtggaaca attgatcgaa gagtcgacta agtaattcat cgaaagggac ggcatgtcgg
     1441 tcggcgtcac ctcccccaat ctgcaaggcc cgaagcgggt caaagatttg atccagcggg
     1501 cctaccgcat tgcccatgtg ttgggcaaag gggaggcctt gtctctcgtc acggcgtcgg
     1561 atgggtttga atcgctcaac gatttgatcg aacaagccat gattgaaaag acgtttgcga
     1621 cctatcagac ggaaattccg attccgctcc aggctcagaa gatcctctat accgtgggac
     1681 cgtccaccgc ctcgccgcct ccggatatca cgtcgacgcg accggcagaa gtgttatcgg
     1741 cctattcacg ccggagcggg cgcgatctcc cgctcttggt cacgcatgcc aagcaagatt
     1801 atgacggggt gccgaataaa aacctcgcct cgaccggatg gagcggattg atctattacc
     1861 aagtggcgta cccctcaggg aacgtctacg tctatcccag tccagccgat gacctgaccg
     1921 tgctctatct cacggtgctc tccgtcctga cgccctatgt gagcttggac gatgaagtct
     1981 ctgttcctcc tggcacctat caatatttga aatatgcgct cgccaagcgg gtcgccgccg
     2041 atcattccat gccgtttggc ccggagaacc aggaaattct ggaacgctgc gaggcggcgt
     2101 ttgagcggaa caacatcaag gcctttccca ttgcgagtac gggcctcaat gccctgtcgt
     2161 ctgggttcag cggctatgac attcggagcg atactacgag gggctactga tgggcacgca
     2221 tcaagccgag ccgatcttcg ggatggggaa ccagcaacgc tcgccgtttc tgtccaccgt
     2281 ctcacggctc aactgcattg tggagccgac ggagaacggg cggcaaccag ccgccattct
     2341 cggtctccct gggctggtgc ggtacgccaa tacgggccag ctgcccgcgc gaggcatctt
     2401 tgtccataag ggagcgctga cgttctactt ggtcgtcggc aatctggtca ttgcgatcct
     2461 gcccaatctt accgtgcaca ccattgccat gctgacgacc gacagcgggc cggtctggat
     2521 ggatgagaac gggacgcaac tgttcgtcaa tgatgccgtt acgccgatca tttataccta
     2581 tgcgaccgga accgccgcga tcattacgga ccccgactat cccagcggcg cacgtggcgc
     2641 ctcctttctg caaggtcgat tttgggttta taccgtcagc gggccaaacg cgggacgctg
     2701 ctatgcgagc gatcagtata acggcctgtc gtgggacggc ctgaacttca tcacgcctgc
     2761 cgcgcgaccg accggaatta ccggtattga tcgatgggct gatgatctcg tgattcgtgg
     2821 gcaaggctcc attgagtggt ggagcggagc gccctcgcag atcagtggcg ccctcggctt
     2881 tcagccttcc tccaatgcca atacgtcagt tggatcgatt gccgaacgcg gtagcgccac
     2941 cgtgggcgat caatacttct ttgtcggcga tcggaacggc agtacgagcg tctttgaagt
     3001 ggtgggctat cagatcgtgc ctgtcagtac gccgaaggtc aacgaagatc tctctcgtct
     3061 gcaagcctcc acggccatct gcaccggcta tatggtcaat gagcatccga tctttcaggt
     3121 cacgattcca ggcgagatta ccgccggggc cgtgacctgg atctatgact cactcaccaa
     3181 gcaatggtcg aagcggggca gcgatggacg cccctactat cgtggcctct ttgccgtggg
     3241 caccaacggc gacgcctaca tcacggacgc ctttaccggc aacctctacc gcatgcacga
     3301 gcagacgtac agcgaagacg gcgaacccat gccatttgag gtcggcagta cgcacctgct
     3361 caaggaaggg gacggatttg cgattgacaa gatccaaatc gatatggaaa ccgggctcgg
     3421 gaatcccatg ccgcccggga acgaccccca cggcattctc tacctctcca aagacggcgg
     3481 gaagacctgg ccgattgaac gctatgtgac gcttgggaag atgggggaat tcaccacgcg
     3541 ggcacaggag tttcaattcg gtcttgctcg cgattgggcg atccggttcc gcattacgga
     3601 gccgattcct cgccgggtcg ccggggccta tttggtcatg aggccggggt acgcatgatt
     3661 ctgatcagca atgcaccgac caacgatccg atttcgaacg agcaaaagaa ctgggtcaaa
     3721 tggttcagtc agctggtcca ggcgttcaac agtctgaccg cgaccgggac gacggatcag
     3781 cgtccgaacc cggcgccctt tcaagggttc atgttcttcg atacgacgct caataagccc
     3841 atttgggcca agaccttaac gcagtatgtg gatgccacag ggaccaatgt atgagctacc
     3901 ccttgcccat gccgacgcag acggtgagtc agtcgccgat tatacgcatt gttgaggcag
     3961 gcggcctcta tttccgatcg gtcctgattc aggaggcggg caccattatt ccgcagcaca
     4021 cgcacgacca tgaccatgtg acgttggtcg gaagtgggcg ggtgcggggc tggcaagacg
     4081 gcgagtgtct tggcgatcga ggaccggggg aagtctttga aattccggca ggcaagggcc
     4141 acgtgttcca gtcattggaa ccgaacacgc tcttaacctg tgtccatgac gtggagagcg
     4201 cactgagcgt gaagcggaaa gggctgtaat tatgccgatg atggcgggtg cggggatggc
     4261 ggcaggcggg atcggactgg gagcgctcgg cgccatgttc ggctcgcagg ccaacaaaaa
     4321 gcaaatcgat ctgatgcggc aagccctcga ctatcagaag ggcattgata cccgcacata
     4381 caacgatctc tcgccctatc gcgatttcgg gaagacgcag ctcaattctt tgacagactt
     4441 tctcgacacc aaagatccgt ccgactacat cgaccccggc tatcagttcc ggctgaattc
     4501 cgggaccaat gcaatcaata acgatgccgc cgacaccggc atgattcaga gcggcgatac
     4561 gctcagagcc ctgaccgagt acggccagaa catggggagc caggaatata acaacgcctt
     4621 caatcggtat ctcggccaag gacaattcct gcaagggctc accggcattg gc
//
LOCUS       DATMTI010000326         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_96654, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000326 DATMTI010000000
VERSION     DATMTI010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96654"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..>1818)
                     /locus_tag="VN039_05425"
     CDS             complement(22..>1818)
                     /locus_tag="VN039_05425"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWV45455.1"
                     /translation="SDDSALGLALDSGGNAVVSGVTGSSTFPLAGTPFQSTRVAHDYF
                     VTKLNSSGTALILSTFFGGVGDDFGAVVALDQFDNIYLTGPINSCRIPNFSHPLQIDR
                     CSLGAPFLHVPVAKLDAGGSSLLGLVVFTGSTGVSAGLGIATDLQGNAYVAGLTRASD
                     FTTQNPFQSTYGGGPEDGFVARISFRPLANSGPDQSKPEGTLVTLDGTGSTGASLTYI
                     WTQVAGPTAALTGATTGSPTFIAPHVSAAGGTTTFELVVCEGTSSNCSDPDAVNVHIT
                     NVNHPPVAEAGPDQTVQEGSPVILDGSASYDPDVEPLTYTWLQIFGPPVTLAYPHSAT
                     PSFTAPPVGASGGQVDFELIVTDARGLNHSDYLSVFISNVNQPPASDAGPDQTRNENT
                     LVTLDGNASSDPDLDTLHFSWSQTGGPVVSLTGANTSNPTFTAPNVGPGGAVLTFQLV
                     VNDGQASGGADTVHVAIQDVNDPPVCTLAQPSVSVLWPPNHTMVAVSIMGVTDPNNSA
                     VTISYPRITQDEPINGLGDGDTSPDAAESGNNILLRAERAGSGNGRVYVVQFRATDQD
                     GASCTGTVKVSVPKSIKDTAVDSGQNYNSFGP"
BASE COUNT          425 a          520 c          492 g          381 t
ORIGIN      
        1 ccggattcat actggacaag cctatggacc aaaggaattg tagttctgtc cactgtcgac
       61 tgccgtgtcc ttgatgctct tcggcactga gaccttgacc gtgccggtac aactggcccc
      121 gtcctgatcc gtcgctctaa attgcaccac atagacacgc ccgtttcctg atcctgcccg
      181 ctccgcacgg agcaggatat tgtttcccga ctctgctgca tccggacttg tgtcaccatc
      241 gccaaggcca ttaatcggct catcctgcgt gatcctggga taggagatgg tgacggcgct
      301 gttgttggga tcggtgactc ccatgatgct cactgccacc atcgtgtggt tcggcggcca
      361 gagcaccgac acgctgggct gagctaaggt acacaccggc ggatcgttca catcctggat
      421 cgcgacgtgt acggtatcgg ctccgccgct cgcctgccca tcgttgacca cgagttggaa
      481 agtgagaact gcgccaccag ggcccacatt cggcgcggta aaggtcggat ttgaagtgtt
      541 cgccccagtc agcgacacga ccggcccacc agtctgactc caactgaagt ggagggtgtc
      601 cagatcagga tcgctgctgg cattcccatc taacgtcacc aacgtgttct catttcgcgt
      661 ttgatccggc ccggcatcgg aggccggcgg ctggttcaca ttcgaaatga agacggagag
      721 gtaatcggag tgattcagtc cacgagcatc ggtcacaatg agttcgaagt ctacctgccc
      781 cccactggca ccaacgggag gcgccgtgaa gctgggtgtg gcgctatgtg gataggccag
      841 cgtcaccgga ggcccaaaga tctggagcca ggtgtaagta agaggttcga catccggatc
      901 gtaactcgca ctgccatcga gaatgaccgg gctgccttct tgtaccgttt gatcgggtcc
      961 tgcctcggcc actggggggt gattgacgtt cgtgatatgc acattaaccg catcgggatc
     1021 gctacaatta ctgctcgtgc cctcacagac cactaactcg aatgtggtcg tcccgccagc
     1081 cgcagataca tgaggagcaa tgaaagtagg agacccagtc gtcgcaccgg tcaaagccgc
     1141 cgttggcccc gcgacttgag tccagatgta ggtgagcgag gcgccggtgg acccggtacc
     1201 gtccaacgtc actaatgttc cctccggttt cgattgatcg gggcctgagt tggcgagagg
     1261 tctaaatgaa attcgtgcaa caaaaccatc ctcaggtcca ccaccatagg ttgattgaaa
     1321 tggattttgc gttgtgaagt cactcgcgcg cgtaagacct gccacataag catttccttg
     1381 cagatcagta gcaattccaa gcccagcact aacacctgtg ctccctgtga aaacaaccaa
     1441 tcccaaaaga ctgctgccgc ctgcatcgag ctttgcgacc gggacgtgca aaaacggcgc
     1501 gcctaacgaa caccggtcaa tctgcaacgg atgcgagaaa ttagggattc ggcatgagtt
     1561 aatcggacca gtgagataga tgttgtcaaa ctgatcaaga gcaacgactg caccgaagtc
     1621 atcaccaaca cccccaaaaa aagtggacag gatcaaagct gtaccggaag aattgagctt
     1681 cgttacaaaa tagtcgtgtg cgactcgagt actttggaat ggtgtcccag caagagggaa
     1741 cgtcgatgat ccggtcacac ctgacaccac tgcatttcca ccagaatcca acgctagacc
     1801 taaggcagag tcatcact
//
LOCUS       DATMTI010000327         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_436358, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000327 DATMTI010000000
VERSION     DATMTI010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436358"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VN039_05430"
     CDS             <1..240
                     /locus_tag="VN039_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45456.1"
                     /translation="DISAKINSAIGDASFAVNGYGNGGRLPAAQPNQVTFASGAIQVY
                     NAKPEQPGITLTRVLTGAARMGTIQAPVGYTTQTG"
     gene            394..1116
                     /locus_tag="VN039_05435"
     CDS             394..1116
                     /locus_tag="VN039_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45457.1"
                     /translation="MAQTHGEFWYPQYFTAATKLLTMFVSSNDPNTGAAPTSIDQGRQ
                     NFDANLDTLSRIFSRRRKLTNIVRAMSNGTSRLALCSVMSVIEPQLVPITSGMITVEL
                     TIPDGFWRDAADTTLATLAAAFSGRVTALDTATAPMNDLEFDIVGPVTNPRITDNESG
                     SWVQYTGTVSAGNTLKIVNSSMSISGVGFTPSIINMTHAGDSNWLTLYPTAPNGVNIT
                     FGGSGTTGATQLQIIGHKKFMR"
     gene            1141..>1664
                     /locus_tag="VN039_05440"
     CDS             1141..>1664
                     /locus_tag="VN039_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45458.1"
                     /translation="MTLKVYDASGNFLNEFPSFTNLQVLDCINDIPAFTLSYNLNAPN
                     ASNFISDSDIQVAVLMDFRDGNGFQEVARYLYEQDTYDPSMAESAIIQANGRGIVALL
                     EQGIVYPQGGVGNPNTNWSFTGASPGKIMNDFINAAKARGCFPALTTSFTSGADSSGN
                     AWTQAFTNAYTAGTS"
BASE COUNT          392 a          477 c          446 g          349 t
ORIGIN      
        1 gacatatctg cgaagataaa ctctgctatc ggagacgcca gcttcgcagt gaacggttac
       61 ggcaatggag gaagactgcc agcggctcag ccgaaccagg ttactttcgc ttcgggtgca
      121 atccaggttt acaacgccaa gccagagcag ccgggtatca cgctgactcg tgtcctcact
      181 ggggcagcgc ggatgggaac aatccaagcc ccggtcggtt acaccacgca gacaggctaa
      241 acagtggcta ccgagacatg gactgtagac gggtttaatc tatccacagt ggcatatgac
      301 atccgcacat ggaacgggct cgacaacatc cctggcgtaa acgtatccgg atctggttct
      361 gggacttccg gtggaacaaa cggtctcttc cagatggcac aaactcacgg tgagttctgg
      421 tacccacagt acttcacggc tgcgacgaag ttgctcacca tgttcgtcag ctcgaacgat
      481 ccgaacacag gggcggcacc tacttcgatc gatcagggta gacagaattt cgatgccaat
      541 ctggacacgc tcagcagaat attctctcgt cgtagaaaat tgacaaacat tgtcagggca
      601 atgtccaacg ggacttccag gttggcgctg tgttctgtga tgtccgtgat cgagccgcag
      661 cttgtcccta ttaccagcgg catgattacc gtcgagctga cgatccctga cggcttctgg
      721 cgggatgctg cggacacaac cctggcgacc ctcgctgcgg ccttctccgg ccgtgtgacg
      781 gccttggaca cggcaactgc accgatgaac gacctggagt tcgatatcgt cgggccggtg
      841 accaaccctc ggatcaccga caacgaatcc ggatcgtggg tgcagtacac cggaacggta
      901 tcggcgggga acaccctgaa aatagtgaac tccagcatgt cgatatcggg tgtgggcttc
      961 acccctagca tcatcaatat gacgcatgcg ggcgactcga actggctgac tttgtatccg
     1021 actgccccaa atggagttaa catcaccttc ggcggcagcg gcacgacggg tgcgacgcaa
     1081 ctccagatca tcgggcacaa gaaattcatg aggtgactgg tgtctactcc cgatctcccc
     1141 atgacgctga aggtatacga cgctagcgga aacttcctaa acgagtttcc aagtttcacc
     1201 aaccttcagg tgctcgactg catcaacgac attcccgcct tcacgctgag ctataatctg
     1261 aacgccccga acgcttccaa cttcatctcc gactcggata tccaagtcgc ggtgctgatg
     1321 gacttccgtg acggaaacgg gttccaggaa gtagccaggt atctttacga gcaggatacc
     1381 tatgaccctt ccatggctga gtcagctatt attcaggcaa acggccgtgg catcgttgca
     1441 ctgctcgaac agggcatcgt ctatccgcaa ggcggagtag gaaaccccaa caccaactgg
     1501 tcgttcaccg gggccagtcc cggaaagatt atgaacgact ttatcaatgc ggccaaggca
     1561 cgcgggtgtt ttcctgcttt gacgacctcg ttcacctctg gtgcggatag ttctggaaac
     1621 gcttggacac aggcgttcac caatgcttat acggcgggaa cctc
//
LOCUS       DATMTI010000328         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_12373, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000328 DATMTI010000000
VERSION     DATMTI010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12373"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VN039_05445"
     CDS             complement(<1..285)
                     /locus_tag="VN039_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45459.1"
                     /translation="MTVSSVLESRHWERESLDQIERNYRALLSVALVLNSQRDMRSLW
                     EAITVEITKAIPWARASITLYDSQTDAFRFYVLATTVPQVALHRDAVVPRH"
     gene            complement(384..1808)
                     /locus_tag="VN039_05450"
     CDS             complement(384..1808)
                     /locus_tag="VN039_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45460.1"
                     /translation="MMKSGLWLSGALLLGLTSIAAADSETPTILPNLFPFPNTTGILK
                     TFNATGKLDLTGPFFQSLGTNGRSCASCHQPGDAWSVSAAHVEDRFERSHGLDPIFRT
                     NDGSNCDHNIDTTTVEGRREAYSLLRTRGLIRIALAVPAGAEFDVVSVKNPYGCSETS
                     TLSMYRRPLPSTNLRFLSTVMWDGRESSPQTGTKPIMFATNPADLISNLAHQSLDAAL
                     GHAQATTAPTQEQQKKIVDFEMALSTAQAIDFGAGSLDVHGASGGPVILSEQPFFIGI
                     NDPLGLNPFKTPFAPVVFTLFTNHWDLAPGHEQHRHHPDRRASILRGQTLFNSHPINI
                     VGVGGLNDATGLPLIKGTCGTCHDTFNVGNHSIGAPLNIGIADVTNPLGVHYLPVITL
                     RHKTDGTEVSTTDPGRALITGKWADIGKFKGPSLRGLAARAPYFHNGSAATLKEAVEF
                     YNIRFSMGLTHQEKADLAAFLSVL"
     gene            complement(2016..2228)
                     /locus_tag="VN039_05455"
     CDS             complement(2016..2228)
                     /locus_tag="VN039_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45461.1"
                     /translation="MSETGIVDRVRSVLEEHGTACSLDDVTQLCPDLTWNQVFLAVDF
                     LSRTGQADVILDPRRGYWVQTRRTAA"
BASE COUNT          480 a          579 c          717 g          463 t
ORIGIN      
        1 gtggcgcggg acgacggcat cgcggtgaag cgcgacctgc gggaccgtgg tagcgaggac
       61 ataaaatcgg aatgcatcgg tctgagaatc gtacagggtg atcgatgcgc gggcccatgg
      121 gatggctttg gtgatctcca cggtaatcgc ttcccacaag ctgcgcatgt cgcgctgcga
      181 gttcagcacg agggcgacgg acaagagtgc gcgatagttg cgttcgatct gatccaatga
      241 ttcccgctcc caatgccttg attcaagcac agaagaaact gtcatgtcca ggtgctcgca
      301 tgcggatttt gaacgagata catgcggagg ttgaggtgcc ggcaagactt tgtcaggcac
      361 ctcggcagat catatccccg tcgtcacagc acactcaaaa acgcggcgag gtcggctttt
      421 tcttgatggg tcaaacccat gctaaagcgg atgttgtaga actccactgc ctccttcagg
      481 gtcgcggcgg agccattgtg gaaatagggc gctcgcgcgg ccaggcctcg gagactcggg
      541 cctttgaact tcccaatgtc ggcccacttc ccggtaatta acgctcggcc tggatctgtt
      601 gtggatactt ccgttccatc tgtcttgtga cgcagagtga tgacgggaag gtaatgaaca
      661 cccaaggggt tcgtcacatc ggcaatccca atattcagcg gagcgcctat ggagtggtta
      721 cccacattga aggtgtcatg acaggtgccg caggtgcctt tgatgagcgg gaggccggtc
      781 gcgtcgttga gcccgcccac gcccacaatg ttgatggggt gtgaattgaa gagggtttgc
      841 cctcggagga tgctcgcccg acgatccgga tggtgcctgt gctgctcatg gccgggggca
      901 aggtcccagt gattggtgaa gagggtgaag accaccggtg cgaaaggggt tttgaaggga
      961 ttgagcccca gtggatcgtt gatgccgata aaaaacggtt gctccgagag gatgacggga
     1021 ccaccgctcg cgccgtgaac atcgagggag cccgcaccga aatcgatcgc ctgagccgtg
     1081 gagagcgcca tttcaaaatc gacgatcttt ttttgctgtt cttgggtggg cgccgttgtg
     1141 gcttgtgcat ggccgagtgc agcatccagc gactgatggg cgagattgga aatgagatcg
     1201 gcgggattcg tggcgaacat gatcggcttc gtgccggtct gtggcgaaga ttcgcgccca
     1261 tcccacatca cggtgctgag aaaccggagg ttggtggagg gcagagggcg tcggtacatg
     1321 gagagcgtgc ttgtctcgct gcacccgtaa ggattcttga cgctcaccac atcgaactcg
     1381 gctcctgccg ggactgcaag cgcaatacgg atcaatccgc gggtgcgcaa gaggctatag
     1441 gcttcgcgac ggccttccac cgtggtggta tcaatgttgt gatcgcaatt ggagccgtca
     1501 ttggtccgaa agatcggatc gaggccgtgc gaccgctcaa accggtcctc gacgtgcgcc
     1561 gccgagactg accaggcatc gccgggctga tgacagctgg cgcagctgcg gccgttggtt
     1621 cccagactct ggaagaaagg accggtcaga tcaagcttgc cggtcgcgtt gaaggttttc
     1681 agaatgcctg tggtattggg aaaggggaag aggttcggaa gaatggtcgg cgtctcggag
     1741 tccgcagccg cgatcgatgt aagacccagc agaagcgcac ccgacaacca gaggccggat
     1801 ttcatcatag tcgctccgtg taaattgtat taggagtgcg tacgagactg tatggccaac
     1861 agacacgacg gcgtgttgcc gtgctggtta tgaatctgtg ggatagacaa aggaagtcac
     1921 gtgtgcgatg ggcacggagg tcgtctcaat agcctttctc gaaggaccgg cgaagattgc
     1981 atcgcagtag cggagactga gacatcggca tgctctcatg cggctgtgcg acgggtttgc
     2041 acccaatagc cccgtctagg atccaagatg acatcggctt ggccagtccg gctcaaaaag
     2101 tcgacggcta agaacacttg attccaggtc aagtcgggac agagctgcgt gacgtcgtcc
     2161 aacgaacagg ctgtgccgtg ttcttccagc acggaccgga cacgatcaac gatacctgtc
     2221 tcactcatga gtcacctcg
//
LOCUS       DATMTI010000329         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_818950, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000329 DATMTI010000000
VERSION     DATMTI010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818950"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1587
                     /gene="rho"
                     /locus_tag="VN039_05460"
     CDS             331..1587
                     /gene="rho"
                     /locus_tag="VN039_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWV45462.1"
                     /translation="MKLQELKSKSPTDLLTFAESLEVENASTMRKQELMFAILKVLAS
                     QDIEIIGEGVVEVLQDGFGFLRSANANYLPGPDDIYISPSQIRRFSLKTGDTVEGPIR
                     GPKEGERYFALLKVNTINFDDPEKIRHKVHFDNLTPLYPNERFKMELDVPTSKDLSSR
                     VIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTD
                     MQRSVKGEVVSSTFDEPAVRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEE
                     FKGTGNSEIVLDRKVADKRIFPALDILKSGTRKEDLLVPRQDLQKIFVLRRILAPMGT
                     MDAIEFLIDKLKQTKTNSDFFESMNT"
     gene            1678..>2296
                     /locus_tag="VN039_05465"
     CDS             1678..>2296
                     /locus_tag="VN039_05465"
                     /inference="COORDINATES: protein motif:HMM:NF021882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45463.1"
                     /translation="MPASADTIYALSSGALPAGVAVLRISGPGAFDALKMLTGRELPS
                     PRKATLCSIRNRNNEVIDQALVITFPAPNSFTSEDCVEIHSHGSRAVMASIFTELENL
                     EGFRTADVGEFSRRAFDNGKMDLLEVEGLADLLQAETEMQRRLAVEQSTGKLSALYDG
                     WASRLTRARALIEAELDFADEEDVPDSVAAQVWDAVAQLRGEIQNH"
BASE COUNT          507 a          640 c          625 g          524 t
ORIGIN      
        1 tttcacccgc agcgcgaagc gttttgcgcg ggatgaaaat acaagacctg cccgtcactg
       61 gcggttgatg aacgaagttc cgaccgtact gatgatcctg attgtcatac ttgtggttgt
      121 gaaacccttc ggttaagacg atgcgacgat aaatttcgcg gaattcgtgc tttcctcttg
      181 cagcggccgt gttcaatcgc tattttcgga ccactcatct tcgtggcatg tgaattattc
      241 agtcatctgc ggtatctccc acagcatctg attcgcgaac acgccctccc ccaaattaca
      301 ttatcaaacg gtctttcctt catggctgaa atgaagcttc aggaacttaa gagcaaatcg
      361 cctaccgact tgctgacttt tgctgaatcc ctggaagttg aaaatgcgag caccatgcgc
      421 aagcaggagc ttatgttcgc aatcctcaag gttctcgcga gccaggacat cgaaatcatc
      481 ggcgaaggcg tcgttgaagt cctgcaggac ggtttcgggt tcctgcggtc cgcaaatgca
      541 aactatctgc ccggtccgga cgatatctat atttcgccct cgcagatccg ccggttctcg
      601 ctgaagaccg gtgataccgt cgaaggcccg atccgcggcc ccaaggaagg cgagcgttat
      661 ttcgcacttc tcaaggtcaa cacgatcaat ttcgacgatc ccgaaaagat ccgccacaag
      721 gttcacttcg acaatctgac gccgctctac ccgaatgagc gcttcaagat ggaactggat
      781 gttccgacct ccaaggacct gtcgtcgcgt gtgatcgacc tcgtcgcgcc gctcggtaag
      841 ggccagcgcg gcttgatcgt ggcgccgccg cgcacgggta agactgttct gctgcagaac
      901 atcgcccatt ccatcacggc aaaccatccg gaatgctacc tgatcgttct cctgatcgat
      961 gagcgtccgg aagaagtgac cgacatgcag cgttccgtga agggtgaggt tgtttcctcc
     1021 acctttgacg agccggcagt ccgtcacgtg caggtcgctg agatggttat cgaaaaggcg
     1081 aagcgtctgg tcgaacacgg ccgcgacgtt gtcatcctgc tcgattccat cacccgtctt
     1141 ggccgcgcct acaacacggt cgttccctcc tccggcaagg tcctgaccgg tggtgtggac
     1201 gccaatgccc tgcagcgccc aaagcgtttc ttcggtgctg cccgtaatat cgaggaaggc
     1261 ggctcgctga cgattatagc cacggcgctg atcgataccg gtagccgtat ggacgaagtc
     1321 atcttcgaag aattcaaggg taccggtaac tcggaaatcg tgctagaccg caaggttgcc
     1381 gacaagcgta tcttcccggc gctggatatt ctcaaatccg gtacgcgtaa ggaagacctg
     1441 ctggtgccgc gccaggatct gcagaagatt ttcgttctcc gccgtattct cgcgccgatg
     1501 ggaacgatgg acgccatcga attcctgatc gacaagctga agcagacaaa gaccaattcc
     1561 gatttcttcg aatcgatgaa tacctgatcc gaacttttag ccggattcat cacaaaatct
     1621 gtaaagccgt atccgttcac tcggatgcgg cttgactgtt ttagacagag gtaaatgatg
     1681 ccagcttccg ccgatacgat ctatgcgctt tccagcggtg ccttaccggc tggcgtcgct
     1741 gttctcagga tcagcgggcc aggggctttt gatgccttga aaatgctgac gggtcgggag
     1801 cttccatcac cacgaaaggc caccctctgc tcgattcgga atcgaaataa tgaggtgatc
     1861 gaccaggcgc tggtaatcac ctttccagcg cctaattctt ttaccagcga ggattgcgtc
     1921 gaaattcata gccacggcag tcgcgcggtt atggcttcga tttttaccga actcgaaaac
     1981 ctcgagggat ttcgaactgc cgacgttggc gaattttccc gtagagcctt cgataacggg
     2041 aagatggacc tccttgaggt cgaaggtctg gcagacctct tgcaggccga gacggaaatg
     2101 cagcggcggc tggcggtgga acagagcacc ggcaaattat cggcgctcta tgatggctgg
     2161 gccagtcgcc tgacgcgggc tcgcgccctt atagaagcgg aactggactt tgcagacgaa
     2221 gaggacgttc cggattcggt ggctgcacag gtctgggacg cagtggcgca gttgcgcggg
     2281 gaaatccaga atcatc
//
LOCUS       DATMTI010000330         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_225566, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000330 DATMTI010000000
VERSION     DATMTI010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225566"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VN039_05470"
     CDS             <1..228
                     /locus_tag="VN039_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45464.1"
                     /translation="ISRQSGVGEILHSIFRYDFWDVDKLADQMVGIATSHSLASELKQ
                     NVKQEYARISWHDVAERCMQMYRTMKTGATV"
     gene            225..1451
                     /locus_tag="VN039_05475"
     CDS             225..1451
                     /locus_tag="VN039_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HWV45465.1"
                     /translation="MSKRGITLYLHVHQPWRVRDYTIFDTAHAHDYFDDGSETDRNNA
                     KVLRKVADKSYRPMNALLEKLLDKYPDFKVSLSITGTFIEQAQAWAPDVIESFQRLVA
                     TGRVEIVAETYYHSLAFFYSREEFERQVEAHKSKIRDLFGVETSVFRNTELAYNDDLA
                     KWADGYGFKGILAEGWDPVLEWRSPNFVYRPAGTEQIKLLLKNYRLSDDLAFRFSNQA
                     WEQWPLTADTYESWANESVHDAQLINLFMDYETFGEHQWADTGIFDFFEHFVGRWLSN
                     PDNTFYTVSEAVDGHEPAGELSMPQTVTWADTERDLTAWLGNSMQQEALRHLYALEED
                     ILRTNDLDLIADWRKLQTSDHVYYMCTKWFTDGDVHAYFSPYESPYDAFLYFMNALRD
                     VRYRLHAYHGRRGLNG"
     gene            1444..>1689
                     /locus_tag="VN039_05480"
     CDS             1444..>1689
                     /locus_tag="VN039_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45466.1"
                     /translation="MARPIVLSNGELHVGINKYGLVHDFYYPYVGLENHSAGISLRHK
                     VGVWVDGRLSWLDDDKAWRFNFRYPHHALIGHITARHD"
BASE COUNT          402 a          427 c          442 g          418 t
ORIGIN      
        1 atttcacgac aatctggagt cggcgagata cttcacagta ttttccggta cgatttctgg
       61 gatgtcgaca aacttgcaga ccagatggtc ggcatcgcga cgtcccactc attagccagt
      121 gagctcaagc agaacgtcaa acaggagtac gcccgtattt catggcatga cgtcgctgag
      181 cgatgcatgc agatgtaccg gacaatgaaa acgggggcaa ccgtatgagc aagcgtggca
      241 taaccctcta ccttcacgtc catcagccat ggcgcgtccg tgactatacg atctttgata
      301 cggcgcatgc ccatgattat ttcgatgacg gcagtgaaac ggatcggaac aatgcaaaag
      361 tactccgtaa agtggctgat aaatcttacc gtccaatgaa tgccttgttg gagaagctgc
      421 tagacaagta tcccgatttc aaggtatctc tgagtatcac cggtacgttc atcgagcagg
      481 ctcaagcttg ggcgcctgat gtgatcgaga gctttcagcg tcttgtcgca acgggtcggg
      541 ttgagatcgt tgcggaaacg tattatcaca gccttgcgtt cttttatagc cgtgaagagt
      601 ttgagcgcca agttgaagcg cataagtcga agattcgcga tttgtttggc gtcgagactt
      661 ccgtttttcg taacaccgaa cttgcatata atgacgatct cgccaaatgg gccgacggtt
      721 acggatttaa gggtatctta gctgaaggtt gggatccggt tctcgaatgg cgcagcccga
      781 attttgtcta ccgacctgcg ggcacggaac agattaagct gctgctaaag aactaccgcc
      841 tgagtgacga cctcgctttc cgcttcagca accaggcatg ggaacaatgg ccgcttacag
      901 cagacaccta tgaatcatgg gcgaacgaat ccgttcatga tgcccagctt attaacctct
      961 ttatggatta cgagacattt ggtgagcatc aatgggccga tactggcatc tttgacttct
     1021 tcgagcattt cgtcggacga tggctgtcga atcccgacaa tacgttttat acggtttccg
     1081 aagctgtcga cgggcatgag ccggccggag agctcagtat gcctcagacc gttacctggg
     1141 ctgatacgga acgagatctg acggcgtggc tcggcaatag catgcagcaa gaagcgctgc
     1201 gacatctcta tgcgctcgaa gaagatatcc tgcgcaccaa cgaccttgat ttgattgccg
     1261 actggcgcaa gctgcaaaca tcggaccacg tgtattatat gtgcaccaaa tggttcaccg
     1321 acggcgacgt ccatgcctac ttcagcccat atgagtctcc ttacgatgca ttcctgtact
     1381 tcatgaacgc cctgcgcgat gtccgttatc gtttacacgc ctatcatggc aggaggggtt
     1441 tgaatggcta gaccgatcgt actttccaat ggcgagctgc atgttggcat caataagtat
     1501 gggcttgtcc atgatttcta ctacccctat gtcggtctgg agaaccattc ggccggtata
     1561 tccctcaggc ataaggtcgg cgtttgggtc gacggccgtc tcagctggct ggatgacgat
     1621 aaagcatggc gcttcaattt ccgttatccg catcatgcgc ttatcggcca tataacggcg
     1681 cggcacgat
//
LOCUS       DATMTI010000331         4426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_436537, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000331 DATMTI010000000
VERSION     DATMTI010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436537"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VN039_05485"
     CDS             complement(<1..458)
                     /locus_tag="VN039_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45467.1"
                     /translation="MGISHGQLETLVDEMLLGIEPRGTHATGLLVVDRKNQPFLEKAD
                     VKASTFVKFRTGLPRRVRTILGHTRLATQGEPHILENNHPIQYNSCYAIHNGHINNDS
                     ELFTEFELDRIAQVDSEIIPALFDKFGLDKAHLALQELDGGYATAVVDPER"
     gene            complement(500..1813)
                     /locus_tag="VN039_05490"
     CDS             complement(500..1813)
                     /locus_tag="VN039_05490"
                     /inference="COORDINATES: protein motif:HMM:NF023645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HWV45468.1"
                     /translation="MGENRLYCEDHRIEAYCAGCDTYMERNTEDEPFVEAVGGGIYCD
                     ECTLLVCQNCHRYYRRGLTWNSDLELNVCRRCLFVGADGTSGESFDEDADMTNKAMQI
                     PTIPGRENVRQCGIEIEGANGTSNGDELALALYGAGLSAIDEMAGYHHGNGSGFAHVE
                     RDASVDWEVVIGPLNPAHQRQVRNLNSVVRIIREHIKDGRLGLDLRAGCHIHVEAART
                     SLTGAFNLNTLFAYLEDVIFRLAAARWPVHRAIMDTHYTAPIPKDLRKLQFARRHNND
                     EDARYYALSFQNYFNRVLSNCHCGAVRYDSWEECECDLGKCTFEFRVFNTTANPRKLH
                     AYMALAQALVAKAMSLGDLDNASDTFPALEFRTRRFKDMPETEQEEIVGLWRDRLAWM
                     FNELPLTDAEKESISYCIRHSELEQVGAEFIESLLPEQQVEEAIA"
     gene            complement(2757..2888)
                     /locus_tag="VN039_05495"
     CDS             complement(2757..2888)
                     /locus_tag="VN039_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45469.1"
                     /translation="MRLSNPSESLGNVKKPEKYRVHGTRIEDLLGKDEANAIRRAVR"
     gene            complement(2899..3786)
                     /locus_tag="VN039_05500"
     CDS             complement(2899..3786)
                     /locus_tag="VN039_05500"
                     /inference="COORDINATES: protein motif:HMM:NF023645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HWV45470.1"
                     /translation="MRDRAFGVEIECGYYGPGMDREPSCSCQCWCDSERDYMRLHNDR
                     FGKRYTQEDIDTFQCEFCRYGCEGCEDSYEYSEGCEVAADLLSRNGFEHWLDDIHEDG
                     SGVEIPSPILRGPQGLRELRNVMQLLSENGFRAGRADGMHVHHDAPEFTDEGLLAHTV
                     ELWEDNFEHITRFVDPARRGTTWASPRHERGYAERYEEFKKSKKIADLPSWRTCALNI
                     MPLRCHEPNIEIRLHEGTLDFRKAAAWIHFGQAFLWAAMRSYQKREVRVCADSLEVLR
                     MANVRGNSRRVLMEVAALS"
     gene            complement(3779..4018)
                     /locus_tag="VN039_05505"
     CDS             complement(3779..4018)
                     /locus_tag="VN039_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45471.1"
                     /translation="MMHPGDDQLYGFEDSDEKELFDIVDRIRAYTCYGALISELSPAL
                     NMKGYIEQGFEDMDDYLAKHAAFEEFMSERSETDA"
     gene            complement(4197..>4426)
                     /locus_tag="VN039_05510"
     CDS             complement(4197..>4426)
                     /locus_tag="VN039_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45472.1"
                     /translation="QSSAACGSLMPTSDEISQRYRLARAVRATEVAADLRISLDALDL
                     LFQLEMELRNGVVPEPDVLLGRVQRIVGSSE"
BASE COUNT          887 a         1349 c         1213 g          977 t
ORIGIN      
        1 cgctcaggat cgacaaccgc tgtggcgtat ccgccgtcca gctcctgaag cgccaggtgc
       61 gccttgtcta gaccgaactt gtcgaacagg gccgggatga tctccgagtc cacctgcgcg
      121 attctatcca gctcgaactc agtgaacagc tcactgtcgt tgttgatgtg gccgttgtgg
      181 atcgcgtagc acgagttgta ctgaatcggg tgattgttct ccaagatgtg cggctcgcct
      241 tgtgtggcta agcgcgtatg cccgaggatt gttctcaccc gacgcggcag gcccgtcctg
      301 aacttgacga acgtgcttgc ctttacgtca gccttctcta gaaagggctg attctttctg
      361 tccacgacga gcaggcccgt cgcatgagtg ccgcgcggct caatgccgag cagcatttcg
      421 tccaccagcg tctcaagctg gccgtgactg atccccatat cgcggggatt cttgaagtgg
      481 attcccatga ttccacacac taggcaatcg cctcctctac ctgctgctca gggagcagcg
      541 attcgatgaa ctccgctcca acctgctcca gctcgctgtg ccggatgcag tacgagatgc
      601 tttccttctc ggcgtcggtc agcggaagct cgttgaacat ccatgcgagc ctgtcgcgcc
      661 ataggccgac gatttcttcc tgctctgtct cgggcatgtc cttgaagcgc cgagtcctga
      721 actccaacgc agggaacgtg tcactagcgt tgtctagatc gccaagggac attgcctttg
      781 cgaccagcgc ctgtgccagc gccatgtatg cgtggagctt ccggggattc gccgttgtgt
      841 tgaacacccg gaactcgaag gtgcatttcc cgagatcgca ctcacattct tcccaggagt
      901 cgtaccgaac agctccgcag tggcagttgc tcagcacccg gttgaagtag ttttggaacg
      961 agagtgcgta gtaccttgcg tcctcgtcgt tgttgtgcct ccgagcgaac tggagcttcc
     1021 gcagatcctt ggggatcggt gcggtgtagt gcgtgtccat gatcgcccga tgcacaggcc
     1081 aacgcgctgc tgcaagccgg aagatcacat cttccaagta tgcgaacagc gtgttcaggt
     1141 tgaaggcacc agtgaggctc gttcgtgcgg cctcaacgtg gatgtgacag cccgctcgca
     1201 aatccaggcc gagcctgccg tccttgatgt gctcgcggat aatgcgcacg acactgttca
     1261 ggttgcgcac ctgtctctga tgagcaggat tgagcgggcc aatgacaacc tcccaatcca
     1321 ctgacgcgtc acgctcgacg tgagcgaatc cgcttccgtt gccgtggtga tagcccgcca
     1381 tttcgtcaat ggcagacagg ccagcgccgt acagcgcaag ggctagctcg tcgccattac
     1441 tggtgccgtt agcgccttcg atttcgatgc cgcactgacg cacgttctcc cggcccggaa
     1501 tggtcgggat ctgcattgcc ttgttcgtca tgtcggcgtc ctcatcaaag gactcaccgc
     1561 tagtgccgtc agctcccacg aacagacagc ggcggcagac attcagctct aggtcagagt
     1621 tccatgtcag cccccgcctg tagtagcggt ggcagttttg gcagacaagt agggtgcatt
     1681 cgtcgcagta gataccacca cctactgcct cgacgaatgg ctcgtcctcg gtattgcgct
     1741 ccatgtaggt gtcacagcca gcgcaatagg cttctatgcg gtgatcctca cagtagaggc
     1801 ggttctcgcc cacgacatag actccacaac cgtctatgtc acagggacgc ccacaggtag
     1861 tgcagactgt gttgtggcct gtgtctacga gctgtgtgtc tgtcgctccg cagttggcac
     1921 aagcgaacgc gagtccgtta cacgaatccg tgtatgccag gtgcagctca ccacaatgag
     1981 gacatgtgaa ctcctcctgc cactccgggt atgtaccgaa gttgaatgga tagtctggat
     2041 tctctcgacc gatacagatg gaagcaggac gacggacaag cactgactct ctgttgggga
     2101 ttcccagtac aaagacttgg agtcctcgct caggatgaac taccagatcg ttctcagcag
     2161 cccactctct gagacgagtg attagagccg gatactccgg gttatctccc gacacagaaa
     2221 taatgccgat agtgttctcg ctctgcggat acgagaccca catgaacggc ggcgggagcg
     2281 cagtggtcag ccaccacgca ttgcttggca cattcgcctc ttcgaatgga atgtcgggtg
     2341 ccgacggact tggatcccag accactggct cggacgacat ctcgttcaga cgccgcgttg
     2401 cctgtagctc ctcattggac atgcacacag gctcaggggg cattactaca cccagacgca
     2461 cgatgccgtt gccgtagccc gtacagtagt ggcctgggtc tgagacactt gatccgcttg
     2521 agtcatccct gactcgatga ctgcttgcgt caagccacac gattgcgttg cagaatgaac
     2581 agcgccagcg tggaatgtca ggtcttgaat agtgttgtcc aggcattaca gccccagagc
     2641 gtgagtcagc atcacgaagc cgggtgcttt ctcccacagc agatgctcaa tgggaaagcc
     2701 agccgtgaac gctagacaac gcaggcacag gcgcctgctg tgtgtgcggc agtgtctcac
     2761 cgcacagccc tgcggattgc attggcctcg tccttgccga gaagatcctc gatgcgtgtg
     2821 ccatgcacgc ggtacttctc aggcttcttg acgttgccta gagactcgct gggattgctg
     2881 agacgcatat gcatcctcct agctgagcgc cgcaacttcc atcagtacgc gacgcgagtt
     2941 tccgcgaaca ttcgccatgc gcagcacttc tagagaatct gcacacacgc gaacttcgcg
     3001 cttctggtac gaccgcattg cggcccacag gaacgcctgc ccaaagtgaa tccaggccgc
     3061 tgccttgcgg aagtcgagcg ttccctcgtg cagcctgatc tcgatgtttg gctcatggca
     3121 ccgaaggggc atgatgttca atgcacaggt gcgccatgag ggaagatccg cgatcttctt
     3181 ggacttcttg aactcctcat aacgctcggc atagccacgc tcatgacgcg ggcttgccca
     3241 tgtggttcca cgccttgccg gatcgacaaa gcgcgtgatg tgctcgaagt tgtcctccca
     3301 cagctcgacg gtgtgtgcga gcagcccctc gtcagtgaac tccggcgcat cgtggtgaac
     3361 gtgcataccg tctgcacgcc cggcccgaaa gccgttctca ctgaggagct gcatgacgtt
     3421 gcgaagctca cgcaacccct gaggcccacg gagaatgggc gagggaatct cgacacccga
     3481 tccgtcctcg tgaatgtcat cgagccaatg ctcgaagccg ttgcgactga gcaggtctgc
     3541 cgctacctcg cagccctcgc tgtactcgta gctgtcctca cagccctcgc agccatagcg
     3601 acagaactcg cactggaagg tgtcgatatc ctcctgtgtg tatcttttac cgaacctgtc
     3661 gttatgcagc ctcatgtaat cccgctcgct gtcgcaccag cactggcagc tacaggacgg
     3721 ctcccgatcc atgccagggc cgtagtagcc gcactcaatc tccacgccga aagcgcggtc
     3781 acgcatccgt ttcgctcctt tctgacatga actcctcgaa agcggcgtgc tttgcgaggt
     3841 agtcgtccat gtcctcgaat ccctgctcga tgtagccctt catgttcagt gcaggactaa
     3901 gctcgctaat caacgcgcca tagcaggtat aggcacgtat gcgatctacg atgtcgaaca
     3961 gctccttctc gtcgctgtcc tcgaagccgt agagctggtc gtcgcctggg tgcatcacgc
     4021 gagcgcctcc tttgcaaggc gctttgcttc gttagccata gccttgaagt caggcgtata
     4081 tgtgattcct gtatgagtca gcacaggcct ctgaatgaga agcgaagcaa tctgctcaag
     4141 tgcagttcgc tccttcacac aatcgcagcc aaactcggga tgacagaact cagccattac
     4201 tcgctgctcc cgacgatgcg ctgcacacgc cctagcagca catccggttc tggcacgact
     4261 ccgttccgta actccatttc gagttggaac agcagatcca atgcatcgag tgagatacgt
     4321 agatccgctg ctacctcggt agctcgcaca gcccgagcca gccgatacct ctggctgatc
     4381 tcatctgacg taggcattag cgagccacac gcggcactag attgcc
//
LOCUS       DATMTI010000332         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_182480, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000332 DATMTI010000000
VERSION     DATMTI010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182480"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..546)
                     /locus_tag="VN039_05515"
     CDS             complement(421..546)
                     /locus_tag="VN039_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45473.1"
                     /translation="MAKGEQRGNREAKKPKKEKPKVIAAAPSLKGTMSSGATGKK"
     gene            complement(613..918)
                     /locus_tag="VN039_05520"
     CDS             complement(613..918)
                     /locus_tag="VN039_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45474.1"
                     /translation="MVKDSDAFRENAENCLHLAESATTEQASIRYKRMSRAWFALAIE
                     QDWLDGHPTQGERATELAGDALDGLSDEGASNNDIVERKNQLIDGPAEFRDTRVDHD"
     gene            complement(1037..>1853)
                     /gene="lpxD"
                     /locus_tag="VN039_05525"
     CDS             complement(1037..>1853)
                     /gene="lpxD"
                     /locus_tag="VN039_05525"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:TIGR01853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWV45475.1"
                     /translation="PRFSGLVPADTFSFVTPFPYAIFAQVLALLYPDAAIPTSTFGTN
                     GISSKANVHASASIGHHVTIDPGASVGPNARIGDFTRIGSNAVIGPDVRLGRDCYIGA
                     NTTITHAVIGDRAIIHPGASIGQDGFGFTIRGGKYVKVPQVGEVVIQNDVEVGANTTI
                     DRGAMRATFIGEGTKIDNLVQIAHNVEIGVHCVIAAQVGIAGSTIIGDFVAIGGHSGI
                     APHLKVGSKAQIGGASGVTCDVPAGERWVGFPARQSRAFFRQYAILKRLAKKK"
BASE COUNT          371 a          516 c          530 g          436 t
ORIGIN      
        1 caagtggcag cggaccggcg gaattggttg atctcataag aagctcaatc caaggactgc
       61 gttgttagac tttgcgtttg tctttttgtt cccggatgtc gagcaggctt cgatcgtttg
      121 acctttccca gagcaagttc agcgttcggc agttcgggag cgggttcggc ttgatggcga
      181 ggatctcgac accgcttcgc ggcggcaact gcctgccccg cgcggccggt cgatcagcat
      241 ggtgtttcag gagccgaggc gcgcacggcc tatgtcccgg acatccaggg cttgatgatt
      301 catgccgatc acgcggccat ctgcgaaccc gaataaaccg tagactgcgg tgaatagttt
      361 aaacgtcgag gcctgcttcc catccaaccg cgccaagcag aagctggatg tggcgctacg
      421 ctattttttg ccggtagctc cactggacat ggtgccctta agacttgggg ctgccgcgat
      481 cactttaggc ttttctttct ttggcttctt tgcttctcgg tttccacgtt gctcgccctt
      541 ggccatgata tcgtcctccg gcccataacg ggcccgagat caccctagca caaacgggcg
      601 ggaagagtgt tcctaatcgt ggtcgacgcg ggtgtcgcgg aactcggccg gtccatctat
      661 caattgattt ttgcgttcta cgatgtcgtt gttgctggcg ccctcatcac tcaatccgtc
      721 gagtgcatct ccggccagtt ctgtggctct ctcgccctgg gtcggatgcc cgtcgagcca
      781 gtcctgctcg atggccaagg cgaaccacgc acgcgacatt cgtttatagc gaattgaagc
      841 ctgctccgta gtggcgctct ccgcgagatg caggcagttt tcggcatttt cgcgaaacgc
      901 atcagagtct ttcaccatcg gaggtaacgc tctttggagg gagtggttcc gatggtcgaa
      961 gctgatcacg ttgcgcggag gaaacggagg acgccgacga actggccatg acgaagaagc
     1021 aggcagtctg gagttgttat ttcttttttg cgagccgttt caaaatggca tattgccgga
     1081 agaacgcccg gctttggcgg gcaggaaacc ccacccaacg ttctccagcc ggtacgtcgc
     1141 acgttacccc gctggcacct ccaatttgag ctttgctacc aaccttgagg tgaggggcaa
     1201 tgcctgagtg gcctccaatg gcaacgaaat caccaatgat tgtcgatccc gcgatgccga
     1261 cttgggctgc gataacacaa tgcactccaa tttcaacatt gtgtgcgatc tgaacgaggt
     1321 tatcgatctt cgtaccctca ccgataaatg tcgcccgcat ggcgcctcga tcgatggtcg
     1381 tattcgcccc aacttccaca tcgttttgga tgactacttc cccgacctgg gggaccttga
     1441 cgtacttgcc tcctcggatg gtgaatccga atccgtcctg tccgattgaa gcgccgggat
     1501 gaataatcgc gcggtcaccg ataacagcat gggtgatagt cgtattggcg ccgatgtaac
     1561 aatcgcgacc gaggcggacg tcaggcccga tcactgcatt tgatcctatg cgcgtgaagt
     1621 ctccgatgcg agcatttgga ccgacggacg cacctggatc gatcgtgacg tgatgtccga
     1681 tcgaagccga ggcatgtacg tttgccttgg aggatatgcc gttggttcca aatgtcgacg
     1741 ttggaatcgc cgcgtccgga tagagcaagg caagtacctg cgcgaaaatc gcgtagggaa
     1801 aaggcgttac aaatgaaaac gtgtcggccg gaacgagccc gctgaatcgg ggc
//
LOCUS       DATMTI010000333         1761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_352136, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000333 DATMTI010000000
VERSION     DATMTI010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352136"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /gene="gltX"
                     /locus_tag="VN039_05530"
     CDS             complement(<1..834)
                     /gene="gltX"
                     /locus_tag="VN039_05530"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWV45476.1"
                     /translation="MSQVRVRFAPSPTGFLHIGGVRTALFNWLFARQQKGTFILRIED
                     TDQSRSTDESIQAIIEGMKWVGLDWDEGPFRQTERMDLYRSYALKLFETGDAYWCVCK
                     AEELEVRRKEAEAKGLSPRYDGRCRNLRITSPLGDAALRFKAPQTGQTVIDDLIKGKV
                     VFDNTVVDDVIILRSNGYPTYNFSVVIDDALMGITHVVRGDDHLTNTPRQILIFVALG
                     FPIPRFGHLPMILGADKTRLSKRHGATSIMAYKDMGYLPDAMLNYLVRLGWSHGDQEL
                     FT"
     gene            961..1665
                     /locus_tag="VN039_05535"
     CDS             961..1665
                     /locus_tag="VN039_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV45477.1"
                     /translation="MAELTQPATVLAVTDLTPHVRQLVLKPKTSKISFQPGQWVSLKL
                     PVGPKPPLNRAYSMADPSSPYGELTLVFDHVPGGLGSNYLYELRSGDEVPLSGPYGNF
                     ILPQPLDRELLLIGRYTGLVPMRCLLKQMFFSKIQTPVLLIALAPNENEVLFHQEWLT
                     MALQYPSFRYLPLVAQNGESEAVEKTLAMLAPLVKGQPKVIPMLCGTKAFVRPLRAYF
                     TEGGYDRKEVKIEAYD"
BASE COUNT          417 a          506 c          418 g          420 t
ORIGIN      
        1 ggtaaagagt tcttgatctc cgtgagacca gccaagtcgg accagataat tgagcatggc
       61 atcgggcaga tagcccatgt ctttataagc cataatcgag gtggcgccat gacgtttgga
      121 gagccgcgtt ttgtcggccc ctaaaatcat cggcagatgt ccgaagcgcg ggatgggaaa
      181 gcccagcgcc acaaagatga gaatctgcct tggcgtgttg gtcaaatgat catctccccg
      241 cacaacatgc gtgatgccca tcaacgcatc gtcgatcacg actgaaaaat tataggtggg
      301 atagccgttt gatctgagaa tgatcacatc gtctacgacg gtattatcaa agaccacctt
      361 acccttgatg aggtcatcaa taaccgtctg gcccgtttgc ggagccttga atcgaagggc
      421 ggcgtccccg agcgggcttg tgatccttag attgcggcaa cggccgtcat agcgaggcga
      481 cagccctttg gcttccgctt cttttcgccg aacctccaat tcttccgctt tgcacacaca
      541 ccagtaggca tcccccgtct caaacaactt caaggcatag ctacgataga gatccatccg
      601 ttccgtttgg cggaatggtc cctcgtccca atcaaggccg acccatttca tgccctcgat
      661 gatggcctga atcgattcat ccgtcgagcg actttgatcg gtatcctcga tgcgaagaat
      721 gaaggtcccc ttctgctggc gcgcaaagag ccaattgaac agagccgtgc gcactcctcc
      781 tatatggaga aatcccgtcg gactgggggc aaaccggacg cgaacctgac tcatgaccgt
      841 gccgtccctt cttcactcat aacagatcga catctatacc atgcagtgaa aatccttgta
      901 catcgtcttg ccacttccat ctcctgatgg cttctgctat gacggtactg cgaatcgatc
      961 atggcggagc tcacgcaacc tgccacggta cttgcggtta ccgatctcac acctcacgtc
     1021 cgccaactcg tgctcaaacc caagaccagc aagatttcct ttcaaccggg acagtgggtc
     1081 tccctcaagc ttccggtagg gccaaagccg cccctcaatc gcgcctattc catggccgat
     1141 ccttcgtcgc cctatgggga gttaacgctg gtatttgatc acgtgcccgg cggactgggc
     1201 tccaattatc tctatgagtt gagatcagga gatgaagttc ccttgtcagg cccttacgga
     1261 aatttcatcc tacctcaacc actcgatcga gagctgctgc tgataggacg ctacaccggt
     1321 cttgtcccaa tgcgatgcct cctcaagcag atgttttttt cgaaaatcca aactcccgtc
     1381 ttattgattg ccctggcacc caacgaaaat gaggtcttgt tccatcaaga atggctcacg
     1441 atggccctgc aatatccctc tttccgttat cttccgctgg tcgctcagaa cggagagtcc
     1501 gaagccgttg aaaagacctt ggccatgctt gcgccgctgg tcaaaggaca gccaaaagtc
     1561 attcccatgc tgtgtggaac caaagctttc gttcgtcccc tgcgtgcata ttttacggag
     1621 ggaggatatg accgcaagga ggtgaagata gaggcatacg actgaagaca ttattcgtta
     1681 aacgttagac tcttcttttg cgtttaacct aaacgtttaa cgaataacac gcgactggac
     1741 aaatcaatgt ccctcccgca c
//
LOCUS       DATMTI010000334         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_54446, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000334 DATMTI010000000
VERSION     DATMTI010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54446"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VN039_05540"
     CDS             <1..478
                     /locus_tag="VN039_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWV45478.1"
                     /translation="GRYKELEPRFYLPRKGRPLVQVGRPGKYTFESGTLYQFQQVTDI
                     HSDLFERAWIAYQNQLGMGIAKEVARNVLPLATMTQFYATTNPRNLMQFLTLRDEVHA
                     LDEIRAVAEVMRDILASTMPLTYNVFERERESWRKLKDLLQDFTVDEIVEKMRDES"
     gene            829..1110
                     /locus_tag="VN039_05545"
     CDS             829..1110
                     /locus_tag="VN039_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45479.1"
                     /translation="MSWSASLVVRAGEPEPRDGDVSLNNVDEVPEHLEQYREALSAAF
                     GILLSGVVGGKDKNYTINLSGHGNEGHEPASGWANDMLSITISQVGGLE"
     gene            1107..1547
                     /locus_tag="VN039_05550"
     CDS             1107..1547
                     /locus_tag="VN039_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45480.1"
                     /translation="MKVGDKISVSVQGIEVAQADVKEVSDGTATLIIPATRVVMATRT
                     ELAPEVTPVNQGAQTIIDGVERAPVAPVEPVQNVSEVPAAEQSQAPTEEKVISSSSVP
                     VPNQTSLESLDPAVQAKIVEIVNAHNAAKQSAEVQEVDGARVED"
     gene            1522..1920
                     /locus_tag="VN039_05555"
     CDS             1522..1920
                     /locus_tag="VN039_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45481.1"
                     /translation="MTAHGSRIDFDTIADVTIVGNVGGRKVEYSGKTASLKVIVDDNC
                     DNMDWFRGRFPLETTSYSLALEQVDGKQLTVKIGKKKIVRTAATYYPSESLVDKRAIE
                     RCRVEVDAPARATSYFEHDDIVFTWTEEIQ"
     gene            2074..>2275
                     /locus_tag="VN039_05560"
     CDS             2074..>2275
                     /locus_tag="VN039_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45482.1"
                     /translation="MAELEQEDPFNEHDLAIQQFIVTARVYDVMLSLLREANPDVARD
                     LLELHRAGHLVATAPSFSGHFLT"
BASE COUNT          639 a          526 c          628 g          482 t
ORIGIN      
        1 tggaagatat aaggagttag agcctcgctt ctatcttcct cgcaagggtc gtccgctcgt
       61 ccaggtgggg agaccaggta agtacacatt cgagtctgga actctttatc aatttcaaca
      121 agtgaccgac attcactccg acctgttcga gagggcttgg attgcctatc agaaccagtt
      181 aggtatgggc atcgccaaag aggtcgctcg aaatgtgctg ccgctggcga ccatgacaca
      241 gttttacgca accaccaacc cgcgcaacct gatgcagttc ctcaccctga gggacgaagt
      301 acatgcgcta gacgagattc gggcggtagc ggaggtaatg cgagacatac ttgcatctac
      361 gatgcccttg acgtataatg tgtttgagag agagcgtgag tcgtggcgca agttgaagga
      421 cttgctacaa gatttcactg tagacgagat tgtggagaag atgagagatg agtcttagag
      481 aagacctcct ttacaccctc aaaggataca acatctggcg tatcgaggta gaccgaaaca
      541 gtcatgtgaa gggccagccg gtcatcaagg tgactatcta cacgacaacg cacaatccgc
      601 acgagtttac gtttgctgat gcgaacaaga tgacgacaca gcatatgaca agtgtcatca
      661 aatcatatct agaacacaga gtagaggcag cagtatgaac gagtgtgtgt ctgattcaga
      721 agttacagac gcgccggtac tgagaagcat tgccgatagt attgaaccac tctacccaag
      781 cgcagcaagt actttgcgag gtatcgcaag aaagattgag gagaacatat gagttggagc
      841 gcaagtttgg ttgttcgtgc aggcgaacct gagccacgcg acggagacgt cagtctgaac
      901 aatgtcgatg aggttccaga gcacctagag cagtatcgag aggcactttc tgccgctttc
      961 ggcatcctac tctcaggagt ggtcggaggc aaggacaaga actatacgat aaacttgtca
     1021 ggtcacggaa acgagggcca tgagcctgca tccggttggg ccaatgacat gttgagcatc
     1081 actatttcac aggtcggagg attggaatga aggtaggaga caagatttcg gtatctgttc
     1141 aaggtatcga ggtagcacag gcagatgtca aggaagtatc tgatgggaca gcgacactga
     1201 ttataccggc gactcgcgtt gtcatggcca ctcgtaccga gttggcacct gaggtaactc
     1261 ctgtgaacca gggtgcgcag accatcatag atggtgttga gcgcgcgcca gtagcgcccg
     1321 tagagccagt gcaaaatgtt tctgaggtac cagccgcaga acaaagccag gctcctacag
     1381 aggagaaggt catctcgtca tcttcggttc ctgtacctaa tcagacatca ttggagtcgc
     1441 tcgacccggc ggtacaggcg aagattgtcg aaattgtcaa tgcacacaat gctgcaaagc
     1501 aatcagcgga agttcaagaa gttgacggcg cacgggtcga ggattgactt cgacaccatt
     1561 gctgacgtca ccattgttgg caatgtcggc ggtaggaagg tcgagtacag cggcaagaca
     1621 gcatccttaa aggtcatagt ggacgacaac tgtgacaata tggactggtt caggggcagg
     1681 ttcccccttg agactacgag ttatagcctc gccctagagc aagtcgatgg taagcaactc
     1741 actgtcaaga taggcaagaa gaagatagtt cgtacagccg cgacctacta cccatccgag
     1801 tctttggtag acaaaagagc aatcgagagg tgccgggtag aggtggatgc ccccgcaaga
     1861 gcgacatctt atttcgaaca tgacgacata gtattcacat ggacagagga gatacaataa
     1921 tggctgaact gacagacaag acagagagtg ccgacccgag gagcgaattc gccaaggggc
     1981 acgaggcggg gtacaggcaa ggattccaag atgcacgttc attgacactt attaccatca
     2041 tgaatgcact tttctcatca gaggcactca tagatggctg aactagaaca agaagaccca
     2101 ttcaacgaac acgacctggc aattcagcag ttcatagtca cggcgcgggt atatgatgta
     2161 atgctatctc tgttaagaga agcaaaccca gatgtggcaa gagacctttt agagttgcat
     2221 cgcgcgggac atcttgtcgc aacggcacct agtttcagcg gacacttctt gacgg
//
LOCUS       DATMTI010000335         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_649108, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000335 DATMTI010000000
VERSION     DATMTI010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649108"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VN039_05565"
     CDS             <1..381
                     /locus_tag="VN039_05565"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HWV45483.1"
                     /translation="EQGFVIRDRRASGGAEAAPTASSPSKEAKPSVAAQTEPSHAAPA
                     PPVTFSSFVISLGSSSLMLMGEQLDPNQEPIPVNLPQAKEIIDLLSVLEDKTKGNLTP
                     DEQTVLRDMLYALRMKYVTLAPPK"
     gene            523..2856
                     /locus_tag="VN039_05570"
     CDS             523..2856
                     /locus_tag="VN039_05570"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV45484.1"
                     /translation="MPELNHPIPAVGPASEGSAVGHLTAVGSQRRRASDGQLKQPDRK
                     PPHLRPVWIVLILLIPCVVLTLYYSQVVAPGSEESGFFLPTTSYALVLLLVNLDLVGF
                     VVLVLLLSRNLIKAYFERRHRLVGSGFRTKLIAAFIGFSLIPTLLLAFVASGLVNKAV
                     DVWFSEHIEKVMKDSYEVARMQHAGHVALAVNSARAISQELFREDMLTPVQRDLLIAA
                     MARKRMEYGVAGIEVFSSKMETLTKSLDSEIPSGVLDLPISQLVLQVINGKQEFTSVQ
                     EAQTGRLVRAAIPVASGSRRGELEGVVVVETYVPESLLTKMEGIGRQYTAYKQIKAMK
                     NPIKAGAYLFVAVVAVLILFSATWFGFYVARGITVPIQRLAEATEAVAQGDLSVQIDA
                     KATDEIGTLIESFNRMTQDLQGSKSKLEETNLTLRNTNVELDRRRAYIETVVDTIAAG
                     LLSIDRSGMITTFNPSAERILGLAADRFRGRSANEVFKEFGLDSFQTAYDRMLADERD
                     DLDLEGQLDIQGKLITIGLKGSRMRDEANKDLGFVLVFEDLTELIKAQKVAAWQEVAK
                     RVAHEIKNPLTPIQLSAQRLRKKFFEQSPDLDRVFDDATNVIINEVGSLKEMLDEFSK
                     FARLPAPQMTRQSLHDVVREVATLYREAQKDIDLIVDLDEDLPSVNFDREQLRRVFVN
                     LFDNAVQAMSQKGRLWVGTKYDTKRRRVVVTVADEGPGIAPEDQERLFVPYFTRKKTG
                     TGLGLAIVRRIITDHEGQIHVGNNHPKGAVFTFDLPV"
     gene            2897..>3061
                     /locus_tag="VN039_05575"
     CDS             2897..>3061
                     /locus_tag="VN039_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45485.1"
                     /translation="MSASILVVDDEEAIRTSLRSILEDEGYDVSVAANGIEALKIYGT
                     DPPDLMILDIW"
BASE COUNT          670 a          805 c          906 g          680 t
ORIGIN      
        1 gaacaaggat ttgtgatacg agatcggcgg gcgagcggag gggccgaggc cgctccgact
       61 gcgtcttcgc cctcaaaaga ggcgaagcct tcggttgcgg cacagaccga gccatcccat
      121 gcggctccgg ctcctccagt cacgttctcg tccttcgtca tctcgttggg ctcgtcgtcg
      181 ctcatgctta tgggtgagca actggacccc aaccaggaac cgatacccgt caacctaccc
      241 caagccaaag aaatcatcga tctattgtct gtcttggaag acaagacaaa aggcaatctg
      301 acccctgatg agcagacggt cttgcgcgac atgctgtacg cccttcgcat gaagtatgtt
      361 acgctggccc cgcccaaata gcatcctatc gtttgattct ccgtcccgcg agctctctga
      421 cggctgcaaa ttgtgcagca ggctttgaga ctgcctcgca agtcggttat agtgctaaac
      481 acctctgttt tcttgtgtcg atggtgccga gaggacgagt gaatgcctga actgaaccat
      541 cccatacctg cggtaggacc tgcttccgaa gggagtgccg tcggtcacct caccgcagtc
      601 ggcagtcaac ggcgtcgtgc ttcggacggc caactcaaac agcctgaccg gaaaccaccc
      661 catcttcgcc ctgtctggat cgtcctgatt cttctaatac cctgcgtggt cctcacgctg
      721 tattactcac aagtggttgc gcctggctcc gaagagagcg gcttcttcct gccgacgacc
      781 agctacgcac tggttctcct cctggtcaat ttggatttgg tcggcttcgt ggtgttggta
      841 cttctattgt ctagaaatct gatcaaggcg tacttcgaac gccggcaccg tcttgttgga
      901 tcaggatttc gtactaaact catcgccgcg tttatcgggt tttcgctgat cccgacgctg
      961 ctcctggcct tcgtcgcgag cgggctggtc aataaggccg tcgatgtctg gttcagcgag
     1021 cacatcgaga aggtcatgaa ggactcgtat gaagtggctc gtatgcagca tgcggggcat
     1081 gtcgcgctcg cggtcaacag tgcacgggcc atttctcagg agctgttccg cgaagacatg
     1141 ctgaccccgg tacagcgcga tctcttgatc gccgcgatgg ctcgaaaacg gatggaatac
     1201 ggcgtcgccg ggatcgaggt gttttcgagc aagatggaga ccctcacaaa atcccttgat
     1261 agtgagattc cgtccggggt gttggatcta ccgatcagtc agctggtctt acaagtcatt
     1321 aacggcaagc aagagtttac ctccgtccag gaggctcaga cgggacggct ggtgcgcgcc
     1381 gccatcccgg tggcctccgg aagccggcgg ggtgagttag agggggtcgt ggtcgtggaa
     1441 acctacgtcc ctgaatccct gttgaccaag atggagggga tcggtcgtca atatacggca
     1501 tacaagcaga tcaaggcgat gaaaaacccc atcaaagccg gggcgtatct ttttgtagct
     1561 gtcgtggcgg tgttgatcct cttcagcgcg acgtggttcg ggttttatgt ggcgcgcggt
     1621 atcaccgttc cgattcaacg gttggcggaa gcgaccgaag cggtcgccca aggcgatctg
     1681 tcggtgcaga tcgatgccaa ggccactgat gaaatcggga cgctgatcga gtctttcaat
     1741 cggatgacgc aagatctgca ggggagtaaa tcgaagctcg aggaaaccaa cctgactctg
     1801 cggaacacca atgtcgagct ggatcgccgc cgcgcttata tcgaaacggt cgttgatacg
     1861 attgccgccg gtcttttgtc gatcgatcgg agcggaatga tcaccacctt caacccatcg
     1921 gccgagcgca ttcttgggtt ggctgccgat cgattcagag gacggtcggc caacgaggtg
     1981 ttcaaggagt tcggcctcga ctcgttccag accgcctatg accgtatgct ggccgatgag
     2041 cgcgacgatt tggacctgga aggccagttg gatatccaag ggaagctgat tacgattggt
     2101 ctgaaagggt ctcgcatgcg ggatgaggcg aacaaagact tggggtttgt gttggtcttt
     2161 gaagatctga cggagttgat caaggcacag aaagttgcgg cttggcagga ggtcgcgaag
     2221 cgcgtcgccc atgaaatcaa aaatccgctg acccctattc aactgtcggc tcaacgattg
     2281 cgcaagaagt tcttcgaaca atctcccgac cttgaccggg tgttcgacga tgccaccaat
     2341 gttatcatca acgaagtcgg aagcctgaaa gagatgctgg acgagttctc aaaattcgcc
     2401 cgcctgcctg ctccgcaaat gacgcgacaa tccctgcacg acgtagtacg ggaagtggca
     2461 accctctatc gcgaggcgca gaaggatatt gatctcattg tagacctcga cgaagatctg
     2521 ccgtcggtca atttcgaccg ggaacaactg agacgtgtgt tcgtcaatct cttcgacaac
     2581 gcggttcaag cgatgagtca gaaagggcgg ctgtgggtcg gcacgaagta tgatacaaaa
     2641 cgccgtcgtg tggtcgtcac cgtggcggat gaggggcccg gcatcgctcc tgaggatcag
     2701 gaacggttgt ttgtgccgta ttttaccaga aagaagacgg gaaccgggtt ggggttggcg
     2761 atcgtccgcc ggattatcac agatcacgag ggacagatcc atgtggggaa caaccacccg
     2821 aagggagccg tctttacatt cgatttgccg gtgtagggta aaccggtgat gggtaggtgg
     2881 taaggagaaa ggggttatgt cggcatcgat tctagtggtg gacgatgaag aggcgattcg
     2941 cacatccctg cgaagcatcc tggaagatga agggtatgac gtatcggtag ccgccaatgg
     3001 gatcgaggcg ttgaaaatat atggcaccga cccgccggat ctcatgattc tggacatttg
     3061 g
//
LOCUS       DATMTI010000336         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_182559, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000336 DATMTI010000000
VERSION     DATMTI010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182559"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2082
                     /gene="infB"
                     /locus_tag="VN039_05580"
     CDS             <1..2082
                     /gene="infB"
                     /locus_tag="VN039_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWV45486.1"
                     /translation="PAPLVAKQAEVPTKPTVAPPTIGAPTPEPVTGKKKSMAFEAIEA
                     EGQKDKLKKARKPGRPRDDQQQDVKFREDAARWQDLRAIPVQRRDDRSKHIHHSAPSE
                     ITKPRRKSVKVTPRTTVKEFAELIGQRPADIVRKLMDMGQMLTFHQPMNLDAASIFAE
                     EGGVKVEISVEKAGDELLQDIVETSGEERPEPRPPVVTIMGHVDHGKTSLLDAIRQTN
                     VAEGEAGGITQHIGAYTVSVRGKQVTFLDTPGHEAFTAMRARGAKVTDVVILVVAADD
                     GVMPQTTEAIHHAKAAGVPLLVAMNKIDKPAANPDRVKNALSEHGLISEAWGGDTIMV
                     EVSAKQKTGLDTLLEMILLQAEVLELKADPHRQAKGTVIEAKIERGRGPVATVLVQSG
                     TLRVGDAYVVGTFSGRVRALINDRGEKAQQAGPSIPVEVIGLPGVPSAGDVFHVVSNE
                     RVAREIAEERAQKRRAAELTGPAKVSLDDLFAKIQEGSVKELAIVIKADVQGSSEALA
                     GAVEKLSTEAVKLRVIHNGVGGVMESDVLLAAASRAIIIGFNIRPEPKATALAEQQGV
                     DVRLYTIIYDAIADIKAAMEGLLEPTLKERVLGRAEVRQVFTIPKVGPVAGSYVIDGT
                     ISRASAGVRVIRDNVVVYQGKLASLRRFKDDVREVQQGYECGLSIENFNDVKAGDIIE
                     AYAIDKIAAKL"
     gene            2082..2426
                     /locus_tag="VN039_05585"
     CDS             2082..2426
                     /locus_tag="VN039_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWV45487.1"
                     /translation="MEASEFSGNRYHEPTLTESPTMVVGLCTVELFVPGSQSLKDKRQ
                     VIHGLKDRLRGKFNLSVAEVDGQDLWQKAVLGMACVSNENKHVNQVLDQALNLIKSMP
                     AVEVVRTQLELL"
BASE COUNT          607 a          621 c          746 g          468 t
ORIGIN      
        1 ccggcgccgc tagttgcgaa gcaggcagag gtgccgacaa agccgaccgt cgctccgccg
       61 acgatcgggg cgccgactcc agagccagtg acgggcaaaa agaaaagcat ggcattcgag
      121 gccatcgagg ccgagggaca aaaagataag ctgaagaaag cacgaaaacc cggccgcccc
      181 agagatgatc aacaacaaga tgtgaaattc cgagaggatg ccgcccgttg gcaagacctt
      241 cgtgcgattc cggtgcagcg acgggatgat cgatccaagc atatacacca tagcgccccg
      301 tcagaaatca ccaaaccacg tcggaaaagc gtgaaagtga ccccacggac gacggtcaaa
      361 gaattcgccg agttgatcgg tcaacggccg gcggacatcg ttcggaaatt gatggacatg
      421 ggccaaatgc tgacgttcca tcaaccgatg aatctggatg ccgcatcgat tttcgcggag
      481 gaaggtggag tgaaagttga aatatcggtc gagaaagccg gggacgagtt gctgcaagat
      541 atagtcgaga cgagcggtga agaacgaccg gaacctcgac caccggtggt aaccatcatg
      601 ggccacgtcg atcacggaaa gacgtctctt ctcgatgcga ttcgccaaac aaacgtggcg
      661 gaaggggaag ccggcggcat tacccaacat atcggcgcct ataccgtctc ggtacgcggt
      721 aaacaagtga cgtttctgga cactcccggc catgaagcct ttacggctat gcgagctcgt
      781 ggagccaagg tcacggatgt cgttatcttg gttgtcgcag cagacgacgg cgtgatgccg
      841 caaaccaccg aagcgatcca ccatgccaag gcggccggag tgccgctgct cgtggcgatg
      901 aataagatcg ataagccggc cgccaatcct gatcgggtca aaaacgccct ctctgaacat
      961 gggctgatct ccgaagcatg gggcggggat accattatgg tcgaggtatc cgccaaacag
     1021 aaaacggggc tcgatacgct ccttgagatg attttgcttc aggctgaagt gcttgaactc
     1081 aaggcggatc cgcaccggca ggccaaaggc accgtcattg aagccaagat cgagcggggg
     1141 aggggccctg tggcgacagt gttggttcag agcgggaccc tacgagtggg tgatgcgtat
     1201 gtcgtgggaa cattcagcgg gcgtgtccgg gccctcatta atgatcgtgg cgaaaaagcg
     1261 caacaggcag gaccgtccat tccggtggaa gtgatcggat tgccgggcgt tccgtcggcg
     1321 ggagacgtct tccatgtcgt ctccaatgag agagtcgccc gagagatagc ggaagagcgg
     1381 gctcaaaagc ggcgggcggc agaactgacc ggtcctgcga aagtgtcctt ggatgatctc
     1441 ttcgcgaaga tccaagaagg atctgtgaag gaattggcca tcgtcatcaa ggccgacgta
     1501 caaggatcgt ccgaagcatt ggcaggcgca gtcgaaaagc tgtcgactga agccgtcaag
     1561 cttcgtgtga ttcataacgg agtcggtgga gtcatggaat ccgatgtgct gcttgccgcc
     1621 gcatcccggg ccatcattat cggcttcaat atccggccgg aaccgaaggc aaccgcattg
     1681 gccgaacagc aaggtgtcga tgtcaggctt tacacgatca tttatgacgc cattgccgac
     1741 atcaaggctg cgatggaagg cttgctcgag cctaccctga aagagcgcgt attgggacga
     1801 gcggaggttc ggcaggtctt tacgattcca aaggtcgggc ctgtagcagg gtcttatgtt
     1861 attgacggaa cgatctcccg ggcaagcgcc ggagttcgcg tcattcgcga caatgtcgtg
     1921 gtctatcagg gcaagctcgc ttccttgcgg cgtttcaagg atgatgtgcg tgaagtgcag
     1981 caggggtatg aatgcggctt gagcatcgag aacttcaatg atgtcaaagc cggcgatatc
     2041 atcgaggcct atgcaatcga taagattgca gcgaagctct agtggaagcc tcagaatttt
     2101 caggcaaccg ttatcatgag cctactctaa ctgaatcccc gaccatggtc gtggggctat
     2161 gcacggtgga attattcgtc cccgggagtc aatcgcttaa agacaagcga caggtcattc
     2221 atgggctcaa ggacagactt cggggtaagt ttaatctctc agtcgcagaa gtggatggtc
     2281 aggacctctg gcagaaggcc gtccttggaa tggcttgtgt ttcgaatgaa aacaaacacg
     2341 tgaatcaggt gcttgaccag gcgttgaatc tgatcaaaag catgccggcg gtcgaagtcg
     2401 tccgaaccca attggaattg ctctaggcgc cccgcaatgc tg
//
LOCUS       DATMTI010000337         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_54763, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000337 DATMTI010000000
VERSION     DATMTI010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54763"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1880
                     /locus_tag="VN039_05590"
     rRNA            <1..>1880
                     /locus_tag="VN039_05590"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          447 a          486 c          598 g          349 t
ORIGIN      
        1 cgaaagacat gtgagaaaca tgtccgccga atgcccaagg gttcctgagt aaagctaatc
       61 tgctcagggt tagtcgggac ctaagccgag gccgacaggc gtaggcgatg gacaactggt
      121 tgatattcca gtaccacgtc cgtccgttat caccgatggc gggacggggt aggtgaagcc
      181 aacccaggcg atggtcgacc tgggctaacc gcgtagggcg agggataggt aaatccgtcc
      241 ctcatcaagc ctgagacggg aatgcgagcg cgttcagcgc gaagtggctg caaccatgcc
      301 tgctagaaaa gcgtctaggg aggtgggacg tgcccgtacc aaaaccgaca caggtgggca
      361 ggtagagaat accaaggcga tcggaagaac cctcgaaaag gaactcggca aaatggcccc
      421 gtaacttcgg gagaaggggc gccacggtag ggtgataact cttcgcgagt taaagctcga
      481 ggtggccgca gtgaaacggc ccaagcgact gtttaccaaa aacacaggtc tctgcgaagt
      541 cgaaagacga cgtatagggg ctgacgcctg cccggtgccg gaaggtcacg aggagaggtt
      601 agccttcggg cgaagcttcg aatccaagcc ccggtaaacg gcggcggtaa ctataaccgt
      661 cctaaggtga catgcctgaa aaagacagtg tgcagctgtc caggcaaacc gttgcccccc
      721 gcaccagtaa tggtgcggtg cgcatctggc catatgctgg aaaacccggt gtatcccgcg
      781 ctactcgcgc catccgaaag ggtgtctaga gtgacaacgt gacgggtgcg gaaaaccagc
      841 aggaaaggct gatcgcgttt cctgactggg tggtcggttt cgtagacgga gaaggatgct
      901 tctcgatcgg cttcgtgcgg caaccacgcc gtgcggaccg aagcggctac gcaaccggtt
      961 tccaggtttc acacgagttc gcggtgacgc agggcgagca aagcctcggc gtcctccacg
     1021 agctgcgtga ctttttcgga gtcggacagg tgatcatcaa ccggcggtac gacaaccacc
     1081 gggagcactt gcaccgatac gtcgtgcgga agcgcgacga gctcctcgga acggtgatcc
     1141 cgttctttca ggagcatccg ttacggacct cgaagcgtga aaacttcgac aagttcgttg
     1201 agtgcgtcga gatgatctcc gccggccgtc atctgacggt cgacggtctg atccagatcg
     1261 cgaagatcac cgagacgatg aaccggaaga aacccagaac ggaactgatc agaatcctca
     1321 gagactatac gccagaggcc ctggacacag ggtcatgaga tagtcccgtc tgcatggcga
     1381 catgcagggg agcggcatca aaggaagtgc cattcccgtt cggacctgtc gaaaaggaca
     1441 tgtccgaata agacgttcaa gcgaaattcc ttgtcgggta agttccgacc tgcacgaatg
     1501 gcgtaacgag ttgggcgctg tctcttcgag ggatccggtg aaattgtagt ctcggtgaag
     1561 atgccgagta cccgtggttg gacggaaaga ccccgtgaac ctttactaca gctagatgtt
     1621 ggagttcggg attcgttgtc caggataggt gggaggcttt gaagccgcgg cgccagccgt
     1681 ggtggagcca atcttgggat accacccttc gtgttctggg cttctaaccc gctgccgtga
     1741 tccggggcgg gaacagcatc tggtgggtag tttgactggg gcggtcgcct cctaaaatgt
     1801 aacggaggcg cgcaaaggtt ccctcagcac ggttggaaat cgtgcgtaga gcgtaatggc
     1861 ataagggagc ttgactgcga
//
LOCUS       DATMTI010000338         4776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_97263, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000338 DATMTI010000000
VERSION     DATMTI010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97263"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /gene="flgB"
                     /locus_tag="VN039_05595"
     CDS             complement(<1..288)
                     /gene="flgB"
                     /locus_tag="VN039_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HWV45488.1"
                     /translation="MTIFDRTMQLLQRTLDLRSARQRVIASNLANEETPGYRASELTF
                     VDQLQSAHKGRLPIVMTATQSRHFGVHGPQGVQAVTGKLGEVPAGDLPLDAN"
     gene            complement(474..1886)
                     /locus_tag="VN039_05600"
     CDS             complement(474..1886)
                     /locus_tag="VN039_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV45489.1"
                     /translation="MNDHEKSNPLHENDDESERILIVDDDPSMRTALMETVKRLGYSV
                     QGAVDGMDAIDRLARFRPWMVLTDLRMPRLNGLELIKEVRARAPQATIVLMTAYGAIE
                     TAVEAMKLGAGEYILKPFSMDVLERVIANLKSGREEGTITGRPSHPTENRALLTQDPG
                     MIRLISTIEGVASSQATVLIQGESGTGKELLARFIHNRSPRAHRPFIAVNCAALPDGL
                     LESELFGHERGAFTGALIRKIGKFEMAHTGTLLLDEISEMNLSLQAKLLRVLQEREVD
                     RIGGRDPVSINIRVIATTNRALYREVEQGRFREDLYYRLNVFPVTVPPLRERTIDIPL
                     LARHFVSQSAMRNGLAQPTLSESASAHLQRLVWKGNVRELENVMERAVLLSGHGPILP
                     EHCPLEQSGETPGQLVRPQSTANGSLWEMERELIFKTLARVKDNRTHAAKELGISIRT
                     LRNKLREYRENERPCSITAP"
     gene            complement(1879..3180)
                     /locus_tag="VN039_05605"
     CDS             complement(1879..3180)
                     /locus_tag="VN039_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV45490.1"
                     /translation="MTNAVTRHPEERELLHAAFRSFDEAAQTLQQSYGALTARVEQMD
                     LELAQSNEALRRQLGDNEAMRMHLDGVLESLSTGVLVTDDCGTISRSNRAAETLLGAT
                     DAELRNRRAMDVLAGAGLGVCDRPQRIGQTVVSIGRVPLRNDSGERSGHLILFQDITR
                     IYQLEEQLQRKERLAAMGELIGRIAHEIRNPLGSVELFASMLQRDLAEHSSAKRYAQQ
                     ISEAVQAMDRLLANLLLYTRPVRMARDWHLAEPLIGESIKLAAHALTRVPVEIRVDIS
                     REVHSIWCHDGQLKQVLVNLIVNAVQAMPNGGVLAIGFSPESPQTLGMSAVRLTVRDS
                     GLGIDPAHRSRIFDPFFTTKDEGTGLGLAIVYSIVEAHQGRIDVESTVGQGTSCSIIL
                     PHPSTGEDPNEGQVGTRSESESNDPLPVEHEAALAEEWSHE"
     gene            complement(3177..>4776)
                     /locus_tag="VN039_05610"
     CDS             complement(3177..>4776)
                     /locus_tag="VN039_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV45491.1"
                     /translation="ALLGLGYTFMAMGKWSDAEHAFAGLRKGSEDEQLLQGATLGLAH
                     ALFREQRLSEAQPIFDIGYRRWPHLLRNDPLSLQRYALVQVKLHHEASARELMLLFYN
                     LYPRHEYAPTALLHVAEGLRTGAKQPLAEFVYALIPSLYPDSASDTTAKLRLAALRAE
                     KMLPAGSNSVGLTVSAMIHEVPIPDQTEASYRSLLEEIATREVGNPMGSEALYYLGKA
                     YEYASDMNRALQTYKEITLRVADKNDPWALKASERLSALLTPWVEAAIASRDDLTVVS
                     LFHRHGAMAEQRYARSPLLPDIAESHRRLGFSREAVRLHQLVIKSHSDSALLESALIG
                     LGKIYLDQRDPQAARKVLERCRFQFPLGKHEGEVVHLLIDAMRQLRDLQGLLHLCRTW
                     LLRHSVHPERPAMYVQLAKTLGELDKLDESALAYEEAFKAGAEQSPDMLVSYADTLSR
                     LNRHERAIAVYQSVLEKKPNVSQADWARLQAAHHWAALKQYDRATVALAELDVTDDPM
                     LNRLSSSLRSSLRTAGRSGRSEGL"
BASE COUNT          983 a         1454 c         1304 g         1035 t
ORIGIN      
        1 atttgcgtca agagggagat ctcccgccgg cacttcaccg agcttgcccg tcactgcctg
       61 caccccctgc ggcccgtgaa cgccgaaatg tcgagactgc gtcgcagtca tgacgatcgg
      121 cagccgtccc ttgtgcgccg actgcaattg gtccacaaaa gtcaattccg atgcgcgata
      181 gcccggcgtt tcttcgttgg ccaggttcga ggcaatcacg cgttgtcgcg cactgcgaag
      241 atcgagggtc cgctgcaaca gttgcatcgt tctgtcaaaa atcgtcatga tcctatgctc
      301 ctttcaaccc gcggcgtttt atccctgcaa ccataatgcc tgtcagagtg ttgaagtgac
      361 ggtcatgcgc gcggcacgtg ccatacccat cggttttcgc tccactcatc cggcccctgc
      421 ttgaccatta ccgtctgtag caaaaacatg ccgaccggca attcttgcct cgttcacggc
      481 gccgtgatcg aacagggccg ttcattctcc cgatactccc gcagtttgtt tcgcaatgta
      541 cgaatgctga tgcccagctc cttcgccgca tgcgtacgat tgtccttcac ccgtgccagc
      601 gtcttaaaaa tcaactccct ctccatttcc catagggatc cgttggccgt cgattgcgga
      661 cgaacaagct ggcccggcgt ttctccgctc tgttcaagag gacaatgttc gggaaggata
      721 gggccatgcc ctgacagcaa caccgcccgt tccatgacgt tctctaattc acggacgttt
      781 cctttccata ccagccgttg caagtgagca ctcgcacttt ccgatagcgt cggttgcgcc
      841 agaccgttcc tcatggccga ctggctcaca aaatgccgtg cgagcaatgg aatatcgatc
      901 gtgcgttcac gcagcggggg aaccgtgacc ggaaagacgt tgagacgata gtacagatcc
      961 tcccgaaagc gtccttgctc gacttctcga tagagcgccc gattggtcgt cgcaatcacg
     1021 cgaatgttga ttgaaaccgg atcgcgcccc ccgatccgat cgacctcacg ttcctgcaac
     1081 acgcgcaaca actttgcctg gagactcagg ttcatttcac tgatctcgtc aaggagcagc
     1141 gtgccggtat gagccatctc aaatttgccg atttttctga tcaacgcacc ggtgaacgca
     1201 ccgcgctcgt ggccgaagag ctcactctcg agtaacccgt ccggtaacgc cgcacaattg
     1261 accgcgataa acggccggtg cgctcgggga cttcgattat ggataaatcg ggctaacagc
     1321 tcttttccgg taccgctctc tccctgaatc aggacggtgg cttggctgga cgccacccct
     1381 tcgatggtac tgatgagacg gatcattcct gggtcttggg tcaagagagc ccggttttcc
     1441 gttggatgag acgggcgacc ggtgatggtt ccttcttctc gtccagattt gagattcgcg
     1501 atgactcgtt ccagcacgtc catggaaaac ggtttcaaga tatactcgcc ggcacccagc
     1561 ttcatggctt cgacggcggt ttcgatggcc ccataagccg tcatcagaac gatcgtggct
     1621 tgaggagcac gcgccctgac ctccttgatc aattcgagtc cgttcagacg cggcatccgg
     1681 agatcggtca gcaccatcca gggacgaaac cgggcaagcc ggtcgatggc gtccattcca
     1741 tcgaccgccc cttgcacgga atagccgagt cgcttgaccg tctccatcag tgcggttcgc
     1801 atcgatgggt cgtcatcaac gatgagaatg cgctcgctct cgtcgtcatt ttcatgaaga
     1861 ggattgctct tctcatggtc attcatgcga ccattcctcc gctaatgccg cctcatgctc
     1921 aacagggagc gggtcgttgg attcagactc gctccgcgta ccgacttgcc cctcatttgg
     1981 atcttcgcct gtcgaaggat gaggcagaat aatcgagcat gaggtgcctt gcccgaccgt
     2041 gctttcgaca tcgatccgtc cttggtgcgc ttcgacgatc gaatacacaa tcgccaggcc
     2101 cagaccggtt ccctcgtcct tcgtcgtaaa gaacggatcg aatatgcgcg agcgatgggc
     2161 cggatcgatg cccaggcctg aatcgcgtac ggtcagccga acagcggaca tgccgagcgt
     2221 ctgcggcgat tccggggaaa aaccgatcgc caagacacct ccgttcggca tcgcctgaac
     2281 tgcattgacg atcaggttca cgaggacttg cttcagctgt ccgtcgtgac accagatcga
     2341 gtggacctcc cgactgatat ccactcgaat ttcaaccggc actcgggtga gcgcatgggc
     2401 ggccaacttg atcgattcgc cgatcaatgg ctcagccaag tgccaatcac gagccatgcg
     2461 aacgggcctg gtgtaaagca agagattggc cagcaagcga tccatcgcct ggacggcctc
     2521 cgagatctgc tgggcatagc gtttggcgga cgagtgctca gcgagatcgc gctgcagcat
     2581 ggacgcaaaa agctccacgc tgcccaaggg attcctgatc tcatgggcga tccggccgat
     2641 cagctcgccc atggctgcaa gcctttcctt gcgttgcagt tgttcttcaa gctgatagat
     2701 gcgagtgatg tcttgaaaca ggatgagatg gccggaacgt tctccggaat cgttgcggag
     2761 cggaacccga ccgatagaga cgacggtttg tccgatgcgt tgcggacgat cacatacacc
     2821 gaggcccgca cccgccaaga cgtccatcgc acgacggttc cgcagttcgg catccgtggc
     2881 gcccagtaag gtctcggcgg cgcgattgct gcgactgatc gtcccgcaat cgtccgtcac
     2941 caacactccg gtcgacaagg actcaagaac cccatccaga tgcatgcgca tggcttcgtt
     3001 atcgccaagc tgccgacgaa gcgcttcgtt gctctgcgcc aactccagat ccatctgctc
     3061 cacgcgtgcc gtgagcgcgc cgtaggactg ttgcaacgtt tgggcggcct catcaaagct
     3121 tctgaatgcg gcatgcagca gttcccgttc ctccggatgc ctggtcacgg cattcgtcat
     3181 agcccctctg acctccccga acgaccggcc gtccgcaaac tcgatctaag ggacgacgac
     3241 agtcgattca gcatcggatc gtccgtgaca tcgagctcgg ccagcgcgac ggtggcacga
     3301 tcgtattgtt tcaacgccgc ccaatggtgg gcggcttgca gacgcgccca atcggcttga
     3361 gacacgttcg gcttcttttc caacactgat tgatagacgg cgatcgcccg ttcatgtcga
     3421 ttcaatcgcg acaaggtgtc cgcataggat acgagcatat cgggcgattg ctccgcgccg
     3481 gctttgaacg cctcttcgta agccagtgcg gactcatcga gtttgtccag ttcgcccagc
     3541 gtcttggcca actggacata catggccggt cgttcaggat gaacggaatg gcgcaagagc
     3601 cacgtacggc acagatggag aagcccttga agatcccgca gttgccgcat ggcatctatc
     3661 agaagatgca cgacctcccc ctcatgcttt ccgagaggga actgaaaccg gcatcgctcc
     3721 aacacttttc tggcggcttg aggatcccgt tgatccagat agatctttcc aaggccgatc
     3781 aaggcagatt ccagcaatgc cgaatcgctg tgcgatttaa tgactaactg atgcagtcgt
     3841 acggcttccc gggaaaatcc cagacgccgg tgagattccg ctatgtccgg caacagaggg
     3901 gaacgcgcat agcgttgctc ggccattgct ccatgtctat gaaacaggct gacgaccgtc
     3961 aggtcgtctc gcgatgcaat cgccgcctcg acccacggcg tcagaagagc ggacaggcgc
     4021 tcagaggctt tcaaggccca tggatcgttc ttgtcggcga cgcgaagcgt gatctccttg
     4081 taggtttgaa gcgctcgatt catgtcgctt gcatattcgt atgctttgcc gaggtaatac
     4141 agcgcctcgc ttcccatcgg attaccgact tctcgagtcg caatctcttc gagcagcgat
     4201 cggtaggagg cctcggtctg gtccggaatc ggcacctcgt ggatcatggc gctgaccgtg
     4261 agcccgaccg aattgcttcc tgccggcaac atcttctccg cacgaagcgc ggcgagccgg
     4321 agtttggccg tcgtatccga tgcgctgtcc ggatagagcg aggggatcag agcatagacg
     4381 aactctgcaa gcggctgttt ggcaccggtc cgcaacccct cggctacgtg cagcagagcc
     4441 gtcggcgcat actcatggcg gggatagaga ttgtaaaaga gcagcatgag ttctcgagcg
     4501 gacgcctcat gatgcagttt cacctgcacg agggcatacc gctgaagaga caagggatcg
     4561 tttctcagaa ggtgcggcca tcgtcgatag ccgatatcga agatcggctg tgcttcagac
     4621 aatcgttgct ctctgaacag cgcatgggcc agccctaatg tcgcgccttg gagcaattgc
     4681 tcgtcttcac ttccctttcg caggccggca aacgcatgct cggcatcgct ccatttcccc
     4741 atggccataa atgtgtaccc aaggcccagc aaagcc
//
LOCUS       DATMTI010000339         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_479820, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000339 DATMTI010000000
VERSION     DATMTI010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479820"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /gene="hemL"
                     /locus_tag="VN039_05615"
     CDS             complement(<1..1245)
                     /gene="hemL"
                     /locus_tag="VN039_05615"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWV45492.1"
                     /translation="MRTSRSAKLFTDAQQLIPGGVNSPVRAFRSVGGQPRFIARAKGS
                     RLYDVDRNVYIDYVLSWGPMILGHAPSAVITAIKSAAGRGTSYGAPTESEISLAREIR
                     NAFPSMGKVRLVSSGTEAVMSAIRVARGFTKRTGVMKFEGCYHGHGDYLLAKAGSGLA
                     TLGIPDSPGVPEDFVKHTLTAPYNDIRTVQQLIKANRDQLACIIVEPIAGNMGVVPPA
                     PDFLAALRQLASDHNILLIFDEVISGFRVGYGGAQALYGIAPDLTVLGKIIGGGLPVG
                     AYGGRKEIMDLIAPVGPVYQAGTLSGNPLAVTAGLATLKQLRVKGIYKKLEERSTALA
                     KGIGEAAKKAGIPVTQTRVGSMLTTFFTPGPVVDWSTAKQSDTKLYGRFFHHMLEQGV
                     YLAPSQFEAAFLSTAHTTQDIEK"
     gene            1374..>1618
                     /locus_tag="VN039_05620"
     CDS             1374..>1618
                     /locus_tag="VN039_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45493.1"
                     /translation="MAAVKVRQEGKVLLVFTLVAVLPDGAILAEKSIVRIVRRICKSS
                     VHGVVEKVGSDSWEKKLPSRTAERHIVSASSTRRWCEL"
BASE COUNT          357 a          438 c          475 g          348 t
ORIGIN      
        1 cttctcgatg tcctgtgtgg tgtgagcagt cgaaagaaag gcagcctcga actgagaagg
       61 cgccaaatag acgccctgct ccagcatgtg gtgaaaaaac cggccgtaca gcttcgtatc
      121 agactgtttc gccgtactcc aatccacaac aggaccaggg gtaaagaagg tcgttaacat
      181 cgacccgact cgtgtctggg tcacgggaat cccggccttc tttgcagcct cgccgattcc
      241 tttggctaga gctgttgatc gttcttcgag tttcttgtaa atccccttca cccgcaactg
      301 cttgagggtt gccagcccag cagtgactgc cagcggatta ccggagagag taccggcctg
      361 atacaccggg ccaaccggtg cgatgagatc catgatctct ttccgtccgc cataggcacc
      421 gaccggcaat ccgccaccga tgatcttccc gagaaccgtg aggtccggtg cgatgccata
      481 gagtgcctgc gcccccccat aaccgacacg gaatcccgag atgacttcgt cgaaaatcaa
      541 taggatgttg tgatcgctgg ccagctgccg cagtgccgcc aggaagtcgg gagcgggagg
      601 gacgactccc atattccctg cgatcggctc gacaatgatg caggcaagtt gatcccgatt
      661 ggctttgatc agctgctgaa ccgtacggat atcattatag ggagcggtga gagtgtgttt
      721 gacaaagtct tcgggtacgc ccggtgaatc cggaattccc aaggtagcca ggcctgatcc
      781 ggcttttgcc aatagatagt cgccgtgacc gtgataacag ccttcgaatt tcatgacgcc
      841 ggtccgcttg gtgaatcccc gcgccacacg gatcgcgctc atgacggctt cggtgccgga
      901 gctgacaagc cggacttttc ccatggaagg gaaggcgttc cgtatttctc gagcgagtga
      961 tatttccgac tcagtcggcg ctccgtaact cgtgcctcgc ccagccgcgc tcttgatcgc
     1021 cgtgatcact gcggaaggag cgtggcctaa aatcatcggc ccccatgaca gtacgtagtc
     1081 gatatagacg ttgcggtcca catcatagag ccgcgatcct ttcgcccgtg cgatgaagcg
     1141 cggttgcccg ccgaccgaac ggaaggcccg cacaggacta ttgacgccac caggaatgag
     1201 ttgctgagcg tcggtgaaga gcttggcaga gcgggatgtt ctcataatgg gggcgcaccc
     1261 taacacgctg ggtaagagcc ggtcaagaaa ctcattgttg tgcgatccga tcgaagagcg
     1321 ccatgggagg tcaaacaatg tcgtaacgat acagttctat cgatgcagat acggtggcgg
     1381 cggttaaggt tcgacaggaa ggtaaagttc tcttggtttt taccctggtc gctgttttgc
     1441 cggacggcgc gattcttgct gagaagtcga ttgtcagaat agtgagacgg atatgcaagt
     1501 catctgttca tggtgtcgtg gagaaggtcg ggtcggactc gtgggagaaa aagctcccct
     1561 cgaggaccgc cgagagacac atagtatctg catcgagcac gagaaggtgg tgcgagct
//
LOCUS       DATMTI010000340         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_776490, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000340 DATMTI010000000
VERSION     DATMTI010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776490"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1852)
                     /locus_tag="VN039_05625"
     CDS             complement(137..1852)
                     /locus_tag="VN039_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWV45494.1"
                     /translation="MGTVSNSSDAQLDRNALAALYEETFRNLEEGTITEGRVVALTKD
                     KVIVDIGYKSEGMIPSDQFSSEELHNLKIGDRLQVYIEECEDADGNLVLSKEKADKMK
                     IWEELEKLYKEEKSIEGKIVSRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGKTF
                     PLKIIKINHRRGNVVVSRRVLLEETRDKKRQTTLANLKEGQLIQGTVKNITDYGSFID
                     LGGIDGLLHITDMSWGRVGHPSELFTVGDKVEVTVLKYDRETGRISLGLKQKSADPWT
                     NVAGKYPIGTRARGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEIRHPSRVVAVGD
                     QVEAAVLNVDPASRKISLGMKQTAPNPWDMIEGKYPIGTRIEGKVKSLTDFGAFVGLE
                     EGIDGLIHISDMSWTKHIKHPSELFKKGQKVEAVVLRIDKEKERLSLGYKQLARDPWD
                     EAIPARYHVGDSVTGKVSKVADFGIFIELEGGVEGLIHVSESGLEPSAKLEEKFKLQD
                     DVTAKIIKVDREERKIALSLRDHQLDWERKQVDDYHSAQGALDQSLGRAAKQSRKRTQ
                     TDDQS"
     gene            complement(1862..>2094)
                     /locus_tag="VN039_05630"
     CDS             complement(1862..>2094)
                     /locus_tag="VN039_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45495.1"
                     /translation="PIQFETGKQKERAETKQFYQQVSRTVIEHIAALGHVPVPKGRVD
                     PAPETPTGRPPTLTTLSEPGNFTISTRQESEE"
BASE COUNT          429 a          615 c          494 g          556 t
ORIGIN      
        1 tcatcagaat caaaaccccc acccccaccg caaacagcca aaagacttta cggaacagac
       61 gacgcttttg agggcgctcg gtctgggtta catcatccgg cattgcacat gacctgtctc
      121 gcgatgcgaa accaacctaa ctttgatcgt ccgtctgcgt tcgtttccgg ctctgcttgg
      181 ccgcccgacc gagactctgg tccagtgcac cttgtgcaga gtgatagtca tcaacctgct
      241 tgcgctccca gtccaattga tggtcgcgaa gactgagtgc tatcttgcgt tcctctcgat
      301 caactttgat tatcttcgcc gtgacgtcgt cctgcaactt aaacttttct tccagcttcg
      361 cggaaggctc aagaccggat tcgctgacat ggatgagacc ttccactccg ccctccaatt
      421 cgatgaagat tccaaaatcg gcgactttgg acaccttgcc ggtcaccgaa tcgccgacgt
      481 gatatcgcgc gggaatcgcc tcatcccagg gatcacgagc caactgtttg tatcccaacg
      541 aaagccgctc tttctctttg tcgatgcgta acacgacagc ttccactttt tgcccttttt
      601 tgaacagttc agaaggatgc ttgatatgct tcgtccagga catgtccgaa atatggatga
      661 gcccgtcgat cccttcttca agtccgacaa aggcgccgaa atcggtcagg ctcttcactt
      721 ttccttcgat acgggtgccg atcggatatt tgccctcgat catatcccag gggtttgggg
      781 ccgtctgttt catgcccaat gagatcttgc ggctcgcggg gtctacgttg agcaccgcgg
      841 cttccacttg atcaccgacc gccacgactc tcgatggatg tcggatttcg tgcgtccacg
      901 acatctccga aacatgcacc agtccctcga ctcccggctc aagttccacg aacgccccgt
      961 aatcggtcag gctgacgaca cgaccacggg ccctggtccc gatgggatac ttgcctgcga
     1021 cattggtcca gggatccgca ctcttctgct tgagccccag agaaatacga cccgtttcac
     1081 gatcatattt caacaccgtt acttcaacct tgtcgcccac cgtaaacagt tccgatggat
     1141 gtccgactcg accccaggac atatcggtaa tatgcagcaa cccgtcaata ccgccgaggt
     1201 cgataaacga cccgtaatcg gtgatgttct tgacggtacc ctggatgagc tggccttctt
     1261 tgaggttcgc cagtgtcgtt tgccgttttt tatccctggt ttcttccaac agcacgcggc
     1321 gtgacacgac cacattgcct cgcctgtggt tgattttgat gattttgaga ggaaaggtct
     1381 ttccaacaag gccatccaga tcacgaaccg gatgaagatc tatctgcgag ccgggcaaga
     1441 atgctttgac gccgatatcg accatcatgc cgcctttgat gcgtgagaca atcttgcctt
     1501 cgatactctt ctcttctttg tagagttttt cgagttcttc ccaaatcttc atcttgtccg
     1561 ctttttcctt ggaaagaacg agattgccgt ctgcatcctc acattcttcg atatacactt
     1621 ggagccgatc gccgatcttg agattgtgaa gttcctcgga agagaactga tcgctcggga
     1681 tcatgccctc tgatttataa ccgatatcaa cgatgacttt gtccttggtc agagccacca
     1741 cacggccttc tgtaatcgtg ccctcttcca agttacggaa ggtttcttcg tacaacgccg
     1801 ccaatgcatt ccggtctaac tgagcgtcac tgctgttgga taccgtaccc atatgaacgt
     1861 cctactcttc cgactcttgt cgggtgctga tggtgaagtt gccgggctca ctcagcgttg
     1921 tgagcgtcgg cggtcggcct gtcggtgttt cgggggccgg gtcaactctc cccttgggaa
     1981 caggaacgtg acctaaggct gcaatgtgct cgatcaccgt gcggctgacc tgttgataga
     2041 actgtttcgt ttccgccctc tctttttgtt tccccgtttc gaactgaata ggat
//
LOCUS       DATMTI010000341         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_182963, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000341 DATMTI010000000
VERSION     DATMTI010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182963"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..260)
                     /locus_tag="VN039_05635"
     CDS             complement(12..260)
                     /locus_tag="VN039_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45496.1"
                     /translation="MTDEILKAYKEVESAVERYIRLLHDHVAMLQNIEPPGSDKVVRL
                     TAGSKAMTDSAAIYLSYAKYVAYGMPSSEEMVEDEIQG"
     gene            299..1477
                     /locus_tag="VN039_05640"
     CDS             299..1477
                     /locus_tag="VN039_05640"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HWV45497.1"
                     /translation="MRRLNATVSPLLLDAINELVERERFSQERLARAVAELSRRFTKD
                     RTHLSRTYLDDDLLRAAYLQYFLPVNLAKVQLLLDEMPAPEPVKGFSVLDIGSGPGTA
                     TLAVLVRWHQRKLPGALSVTAVDSSSRGLGQAGLLWDRYCRMAGIQEADLQTYVGNLE
                     RREWSEQVRQKGPFDLIILANCLNEIHTKGTDLIEAKTGLVVECLSLLTPPGTIMIVE
                     PALRETSRALHQVRDRLLQEKRCTIYSPCLHEGNCPALEKPDDWCHEERAWEPPVSIQ
                     EIDEEVGFIKDALKFSYLLLRKDGKTIVDRKPDVYRVVSELRVMKGEKRAWLCNEQGR
                     QEVGRQDRLASPHNEAFDHWHRGAIVQIERIIRKERGGKVSALGRIEHDAAVHIMRSV
                     "
BASE COUNT          385 a          391 c          454 g          359 t
ORIGIN      
        1 tagacggtgt tctatccttg gatctcgtcc tcgaccattt cctcgctgga cggcatgccg
       61 taagccacat actttgcata ggacagatag attgccgcac tgtccgtcat ggcttttgaa
      121 ccggccgtca gtcgaacgac cttgtcggag ccggggggtt cgatgttctg cagcatcgcg
      181 acatggtcgt gcagaagcct gatataccgt tccacagccg attcgacttc tttataagcc
      241 ttcaggattt catccgtcat agttgtcctc cgtaataggc cgagcataca ctaggctcat
      301 gcgacgcctc aacgccacgg tttcaccatt actccttgac gccatcaatg aactagttga
      361 gagagaacga ttttcacaag agagattggc gcgggccgtg gcggaattgt ccaggcgctt
      421 taccaaagat cggactcatt taagccgaac atatttagac gacgacctct tgagggccgc
      481 gtaccttcag tattttctgc cggtcaatct tgccaaagtc cagttgttgc tcgatgagat
      541 gccggctccg gaaccggtca aaggattttc cgttctagac attggctcgg ggccggggac
      601 tgcgacgtta gcagtcctcg tccggtggca tcagcgaaag ttgcccggtg cgttatcagt
      661 taccgctgtt gatagttcgt cgagaggtct cgggcaggcc ggactattgt gggatcgata
      721 ctgtcgaatg gccggtattc aagaggcaga cttacagacg tatgtgggaa atttggaacg
      781 gagagaatgg tctgagcagg tgaggcagaa ggggccgttt gacctcatca ttcttgccaa
      841 ctgcttgaat gagatccata caaaggggac agacctaatc gaagcaaaaa ctggcttagt
      901 ggtggagtgt ttatcgctgc tcacaccgcc cggcacgatc atgatcgtag aaccggcctt
      961 acgagaaacc tctcgcgcgc tgcatcaggt gcgcgaccga ttgctccaag agaagcgttg
     1021 caccatttat agtccatgcc ttcacgaggg gaactgtcca gcattagaga agcccgacga
     1081 ttggtgccac gaagagcgag cgtgggaacc gccggtttcg atccaggaaa tcgatgaaga
     1141 ggttggattc atcaaagacg cgctgaagtt ctcgtatcta ttgttgcgaa aagatggaaa
     1201 gaccatcgtc gatcggaagc cggatgtcta tcgcgtggtc agtgaattgc gagtcatgaa
     1261 gggggagaaa agggcttggc tctgcaatga acaaggccga caggaagtcg ggcgacagga
     1321 tcgcctggcg tctcctcaca acgaagcgtt cgatcattgg caccgcggcg cgatcgtgca
     1381 gattgaacgg atcattcgaa aagagcgagg aggaaaggtg tcagcgttag gaaggatcga
     1441 acacgacgct gccgtccata tcatgagatc ggtctgaata aagttcgaat aataagtaac
     1501 gaacgctgcg cggggctctt ccacctcgcc agagtaacgc aaatttgatc atccgaactc
     1561 attcgggcca ggaggccacg gtttgagga
//
LOCUS       DATMTI010000342         9266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_183011, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000342 DATMTI010000000
VERSION     DATMTI010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183011"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1058)
                     /locus_tag="VN039_05645"
     CDS             complement(33..1058)
                     /locus_tag="VN039_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45498.1"
                     /translation="MASGVTQIADVVVPELFTPYVQQLTEQKSRLVQSGAITRDSVLD
                     NDLMGGGLTFNEPSFKDLDNDADNIATDDPTAKSTPFKTGSATEIQVRLSRNGSWSSM
                     DLAGDLAGADPMMSIANRVAYYWTRREQAAFVATINGIFANNATATDTHHTQNDMTHD
                     ISGASFADGVTNFSAEAFIDATLTMGDSMDRLSMVMVNSVVYGRMQKNNLIDFIPDSE
                     GKINIPTFLGRIVIVDDGVPYTAGVFETWLFGAGAVRQGVGNPKTPTEVWRDPAAGNG
                     SGQSFLYNRKELIFHPVGHAYVGTAPKGGPDNTNAANMLAAATSWQRVFPERKQIRIA
                     RLKTREF"
     gene            complement(1203..2015)
                     /locus_tag="VN039_05650"
     CDS             complement(1203..2015)
                     /locus_tag="VN039_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45499.1"
                     /translation="MALKAVLDNLDGLPADVASEYVEKNGKFELQVEGMKTQGDIDRI
                     QGGLTKERADHKATKDRLSAWGELDPTEVLGKLGRFDELEAAAKGKLDDAGIQKIVDG
                     RLNSVKAPLERQVQQLTAKLGEATTAIEGFQTQNRVRTIGDAVRSAAVKTKVLESAVE
                     DVMMYGERLFEVTEDGQILTRDGVGVTPGVSAEVWLSDMATKKPHWFPGSSGGGAGGG
                     TGSAGGGNNPFSREHWNMTEQGKLLTSNRAKAEQMARAAGTTIGGPMPAPRK"
     gene            complement(2081..3682)
                     /locus_tag="VN039_05655"
     CDS             complement(2081..3682)
                     /locus_tag="VN039_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HWV45500.1"
                     /translation="MSAIDSRHPRYSEWMLVWQKLRDFYEGEERVKDQGELYLHPTEG
                     MILDGMKGQGEAKQLGLKRYEAYKARAVVPEYVETAVELLLGLLHQKPATIKLPPEME
                     PLRTICSANRESLDQFQRRINVEQLITGRFGIMCDIAKDATPTDLPYMALYSTERIIN
                     WDDNDEMAGPNALNLVVLDESKLVRVDFEWKFMNAKRVLQLGPILTNEANDAGFVYKQ
                     GYFSNADSSSVQYVESEMLAPMYRGRTLQEVPFVIGNTKDLLSKPDKPPLNSLCNTCI
                     AIYQQEADYRQNLHMQSQDTLVVIGGTRNPSTDPGQPDALRVGAGSRIDVDQGGDAKF
                     IGVSSLGLPEQRQAIENDRKRAIIKSGELIQSGTGSQQESGSALRTRFTAQTATLNQI
                     ALTGAEAIQYALRLIARWIGADETQVEVIPNLEFVDFGLDGLDFLNLMQAKTLGFPVS
                     YESLHQTAQDRGLTTMDFKSEISQIKTENTTIADIIKPPPPVPPPGAPAPSVPPGIDP
                     TKKTNVPPNPRENPGVPNSKPKGGQ"
     gene            complement(3695..5266)
                     /locus_tag="VN039_05660"
     CDS             complement(3695..5266)
                     /locus_tag="VN039_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45501.1"
                     /translation="MSLVEAPPRVVWSPLPGSQTLALCCPAHILLYHGTRGPGKTDSQ
                     LMRFRRRVGQGYGRHWRGVIFDREYKNLDDLISKSMRWFPEFKDGARFLSSKSDYRWV
                     WPSGEELLFRAIRRDSDYWAYHGQEFPFIGWNELTKYSSDTLFDMMMSCNRSSFLPEE
                     YRAWDDDGEPIPPPPPIPLEVFATCNPFGAGHNWVKKRFIDAGKMGEIVRNCVTVFNP
                     RTQRQEEVVKTQVHLFGSYRENKYLSPEYIAELVTMKDANKRKAWLMGSWDVLAGGMF
                     DDVWDNDLHKMRMFKIPIGWRIDRSFDYGQSKPFSVGWWAESDGSDVLLEDGRTWMST
                     VKGDLFRIAEWYGCEEGKSNVGLRMLASDIAAGIVEREIAMGIHNRVQPGAADNAIWD
                     VLNGNSIAMDMEKNVRVGGQVYRGVTWIRSDKSPGSRKRGWEQIRKRLVKSKGEQVTK
                     ADGTVVTVPRQEPGLFVFTNCKNSLNLMPFMSRDEDDPDDIDTNTEDHIADEWRYRVM
                     SANMGARTGKTTGGT"
     gene            complement(5263..5781)
                     /locus_tag="VN039_05665"
     CDS             complement(5263..5781)
                     /locus_tag="VN039_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HWV45502.1"
                     /translation="MSESTAQQSFWAADAMAPQLTASERVLRDKFIVEYLKDYDAFGA
                     SLRAGFMSTMAIEYAKRFLEEPYVQSEIARLERAAAEDPKAEEEQRKRFILASLTREA
                     NYRGPGSSHAARVAALAKLGNFLDMDGVQKTKNEHLHRGGVMAVPGIASLEDWEKAAK
                     DSQTQLVKDTQT"
     gene            complement(5848..6129)
                     /locus_tag="VN039_05670"
     CDS             complement(5848..6129)
                     /locus_tag="VN039_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45503.1"
                     /translation="MSRSHGLLTLLAVVMLGGCREATKPIVPQTITVTVTKYVPLDSA
                     LTEDCPISLPLNRTVSEAVRIARARRVALEDCTQRMRKIRELQPKADGQ"
     gene            complement(6105..6512)
                     /locus_tag="VN039_05675"
     CDS             complement(6105..6512)
                     /locus_tag="VN039_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45504.1"
                     /translation="MTLTSAWLWKLGLIFLISISLWGWGDHHGNDRGQATQAKADKVL
                     IDKANGERDTANTQIGVLAAKLETISSKSKADKAESDKLRQQAAAAVQDLAKEQSLRK
                     KDLDLWTKQLDALKIKPECGALKELLCPAVMDY"
     gene            complement(6509..6868)
                     /locus_tag="VN039_05680"
     CDS             complement(6509..6868)
                     /locus_tag="VN039_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45505.1"
                     /translation="MTKVFDWWHKHEAWIAFTVVMIAVFGAGYMLNDITNSDECDRRV
                     EDIRQQYERVISAKEQTIELLARSTAQTATQAATASAQAVGAATIATQAAQTAQQAAQ
                     AADKATDSNKKALGKLK"
     gene            complement(6865..7230)
                     /locus_tag="VN039_05685"
     CDS             complement(6865..7230)
                     /locus_tag="VN039_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45506.1"
                     /translation="MATAEALMIDDPSAVLPQIPASGWLGMVGAVIVGGVLWLRRFLS
                     NDSVINSANAAQTEIINLLRDQLAKESERADKAEAARNAAMQTIQQLEQKITDLTLKV
                     SSLEQTVKAQNPATAGVMT"
     gene            complement(7317..7724)
                     /locus_tag="VN039_05690"
     CDS             complement(7317..7724)
                     /locus_tag="VN039_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45507.1"
                     /translation="MAWETAQTFSPSIKNGAGSGATGLIQFMDATAKGLGTTIENLEL
                     LSAESQLYYVKKYFEPYAKRIKTRSDMYMAIFYPKYIGLPEDTVISSGGAVYRQNAGF
                     DLNRDGKITKAECTACIDKMHKMGMAVGCVRNL"
     gene            complement(7804..8052)
                     /locus_tag="VN039_05695"
     CDS             complement(7804..8052)
                     /locus_tag="VN039_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45508.1"
                     /translation="MRLLMGLGWRPDTLYCTAHGKKYRIRFLKHRAVIEYQSDGHWIW
                     WGGSSYAGLRRTEDGCIVAGSLVFGEGRAIGLGKQSKS"
     gene            complement(8067..8273)
                     /locus_tag="VN039_05700"
     CDS             complement(8067..8273)
                     /locus_tag="VN039_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45509.1"
                     /translation="MSLQAKVLELTSRSQAVQATFAGLNQLHMAAVLQHDSRRETELR
                     EEIHATVDALLDVQAEMIQVMEQQ"
     gene            complement(8270..8731)
                     /locus_tag="VN039_05705"
     CDS             complement(8270..8731)
                     /locus_tag="VN039_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45510.1"
                     /translation="MGINIRQKGAEGEREVARSLNSIINAVLKARSIPVPEKDVVQRN
                     QNQSAVGGGDLSNVFGLSVEVKRQEQLAVNTWWAQCEASANRNGEIPVLVYRVNRSKW
                     RVVMYAELVLFSATGTQRSMKVRCEVEWEPFLQWFYHWVDAKISAGETIRS"
     gene            complement(8733..8957)
                     /locus_tag="VN039_05710"
     CDS             complement(8733..8957)
                     /locus_tag="VN039_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45511.1"
                     /translation="MSFFTRSEKLEVVINKLKDAQRVLKEAADEAESADAHGVARRID
                     SHIVRLRTCEGHTRSAIGRLQRRASAKGEA"
     gene            complement(8954..9148)
                     /locus_tag="VN039_05715"
     CDS             complement(8954..9148)
                     /locus_tag="VN039_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45512.1"
                     /translation="MDAEAKKRAGMPKAQAMEIAAKSKDKETRKAYRIGITYAYMNDD
                     NRVPPAKMAELAKHACENPE"
BASE COUNT         1734 a         2819 c         2584 g         2129 t
ORIGIN      
        1 ctttcgaccc cgggcgttca tggaccggcg gtttagaact cgcgggtctt caggcgagcg
       61 atgcggatct gcttgcgctc cgggaacacg cgctgccagg aggtagccgc tgcaagcatg
      121 ttggcggcgt tcgtgttgtc cgggccaccc ttcggtgccg tgccgacata ggcatggccg
      181 accggatgga agatcagctc cttgcggttg tagaggaacg actggccgct gccgttgccc
      241 gccgccggat cacgccacac ttcagtcggg gtcttcgggt tgcccacgcc ctggcgcacc
      301 gcacccgcac cgaacagcca ggtctcgaac acgccggcag tgtacggaac gccgtcgtcc
      361 acgatgacga tgcgaccgag gaacgtcggg atgttgatct tgccttccga gtccgggatg
      421 aagtcgatca ggttgttctt ctgcatgcgg ccgtacacga ccgagttgac catcaccatc
      481 gacaggcggt ccatgctgtc acccatcgtc agggtggcgt cgatgaacgc ttcggcgctg
      541 aagttggtga cgccgtccgc gaacgatgca cccgagatgt cgtgggtcat gtcgttctgc
      601 gtgtggtgcg tgtcggtcgc cgtcgcgttg ttggcgaaga tgccgttgat cgtggcgacg
      661 aaggcggcct gctcgcgacg cgtccagtag tacgccacgc ggttggcgat ggacatcatc
      721 gggtcggcac ccgcgaggtc gcctgccagg tccatcgagc tccacgagcc gttgcggctc
      781 aggcggacct ggatctcggt cgcgctgccg gtcttgaacg gagtggactt cgcggtcgga
      841 tcgtcggtcg cgatgttgtc ggcgtcgttg tccagatcct tgaacgacgg ttcgttgaag
      901 gtcaggccgc cgcccatcag gtcgttgtcg agcacgctgt cgcgcgtgat cgctccggac
      961 tggaccaggc gggacttctg ttcggtgagc tgctggacgt acggcgtgaa gagttccgga
     1021 acgacgacgt cagcgatttg ggttacgcca ctggccatga ctggctcctt ttgactgatg
     1081 ggttcaagta gaaactcgaa gcctcagtcc catgacctgg cccggttgta tgtgatatgg
     1141 ctcatgccac acgccacgca atgtagcatg agccatatcg ttgcgcaagc gcaaatgttt
     1201 gattatttgc gcggtgcagg catcggcccg ccaatcgtgg tgccggcagc gcgtgccatc
     1261 tgctccgcct tggcacggtt ggaggtgagc agcttgccct gttcggtcat gttccagtgc
     1321 tcgcgactga acgggttgtt cccgccgccg gccgacccgg tgccgccacc cgcaccgccg
     1381 ccgctcgagc cggggaacca atgaggcttc ttggtcgcca tgtcggacag ccagacttcc
     1441 gcggaaacac ccggcgtcac accgacgcca tcgcgagtca gaatctgacc atcttcggtg
     1501 acttcgaaca ggcgttcgcc gtacatcatg acgtcttcga cagcgctctc cagcaccttc
     1561 gtcttgacgg cagcggaacg cactgcatcg ccgatcgtcc ggacacggtt ctgcgtctgg
     1621 aagccttcga tggcagtcgt cgcttcgccc agcttcgcgg tcagctgctg cacctggcgc
     1681 tcgagcggtg ccttgacgct gttcagacga ccgtcgacga tcttctgaat gccagcatcg
     1741 tccagcttgc ccttggccgc agcctcgagt tcatcgaaac ggccgagctt gcccagcacc
     1801 tccgttggat ccagttcgcc ccaggcggac agacgatcct tggtagcttt gtggtcagca
     1861 cgttccttgg tgagaccgcc ctggatgcga tcgatgtcgc cttgggtctt catgccttcg
     1921 acctggagtt cgaacttgcc gttcttctcc acgtattcgg atgcgacgtc tgcggggaga
     1981 ccgtccagat tgtcgagaac tgctttgagt gccatgactc ttcctcttct ttgttatgcg
     2041 gcgcatgccg cgttaatgac cacagtcatc tgtggccttg ttactgtcca cccttcggct
     2101 tgctgttcgg cacgccgggg ttctccctcg gattgggagg gacgttcgtt ttctttgtcg
     2161 gatcgatgcc aggcggaacg cttggagcgg gcgcacccgg cgggggaacg ggcggtggag
     2221 gtttgattat gtccgcgatc gtggtgttct ccgtcttgat ctgcgagatc tcgctcttga
     2281 agtccatcgt cgtcaaacct cggtcttgcg cggtctgatg gagactttcg tacgagaccg
     2341 ggaagcccag cgtcttggct tgcatcagat tgaggaagtc caggccgtcc agaccgaagt
     2401 ccacgaactc gaggttcgga atgacttcca cttgcgtctc gtctgcgccg atccaccgag
     2461 cgatcaggcg aagcgcgtat tggattgcct ccgcgccagt gagcgcgatt tgattcaggg
     2521 tcgcggtctg tgcagtgaag cgcgtgcgta gtgcgctacc gctttcctgc tgcgaccctg
     2581 tgccgctctg gatcagctcg ccgctcttga taattgcacg cttacgatcg ttctcgatcg
     2641 cttggcgctg ttccggcaag cccaacgaac tcacaccgat gaacttggcg tcgccccctt
     2701 gatccacatc aatacgcgaa cccgctccca cgcgcagtgc gtcaggctga ccggggtcgg
     2761 ttgagggatt gcgtgtgccg ccaatcacga caagcgtgtc ttgcgactgc atgtggagat
     2821 tctggcggta gtctgcctct tgctgataga tcgcgatgca ggtattgcac agcgagttga
     2881 gcggaggctt gtcgggtttg gagaggagat ccttcgtatt gccaataacg aagggaacct
     2941 cttgcagcgt cctgccacga tacatcgggg cgagcatttc gctctccacg tactgcacgg
     3001 aagacgaatc tgcgttggag aaatatcctt gcttgtagac gaaccctgcg tcgttggcct
     3061 cgttggtgag aatcggaccg agttggagaa cgcgctttgc gttcatgaac ttccattcga
     3121 agtccacacg caccagcttg ctttcatcca gtaccacgag gttgagggca ttcggacccg
     3181 ccatctcgtc gttgtcgtcc cagttgatga ttcgctccgt ggaatagagc gccatgtacg
     3241 gaagatcagt cggagttgcg tccttcgcga tgtcgcacat gatcccgaaa cgacccgtga
     3301 tcagctgctc cacattgata cgacgctgga actgatcgag ggattctcga ttggccgagc
     3361 agattgtgcg gaggggctcc atctccggag ggagtttgat cgttgcaggc ttctggtgaa
     3421 gcagaccgag cagaagctcc acagcggtct cgacgtattc gggaacaacg gcgcgcgcct
     3481 tgtaggcctc ataacgcttg agacccagct gcttggcttc tccttggccc ttcatgccat
     3541 cgaggatcat gccctccgtc ggatgcagat aaagttcacc ctgatctttg acgcgttcct
     3601 cgccctcgta gaaatcacga agcttctgcc aaacgagcat ccactcgctg tatcgaggat
     3661 ggcggctgtc aattgcagac atatctgttc tccgttaggt gccgccggtc gttttgccag
     3721 tacgagcacc catgttggcg gacattacac ggtatcgcca ttcgtccgca atatggtctt
     3781 ccgtgttcgt atcaatatca tctggatcat cctcatcgcg agacatgaac ggcatcagat
     3841 tgagcgaatt cttacagttt gtgaaaacga agaggcctgg ctcttgacgc ggcaccgtga
     3901 ccacagtgcc gtccgccttc gttacctgtt cgcccttcga cttcacaagg cgcttccgga
     3961 tctgctccca gcctcgcttt cgactaccgg gtgacttgtc agagcgaatc caagtcactc
     4021 cgcgatatac ctgcccaccg acacggacgt tcttctccat gtccatggcg atgctgtttc
     4081 cattcaggac gtcccagatc gcattgtctg ctgcgcccgg ctgcacgcgg ttgtgaatgc
     4141 ccatggcgat ctcgcgctcc acaatgcccg cagcaatatc gctcgcgagc atacgcagac
     4201 cgacgttact cttcccttcc tcgcagccgt accattctgc gatgcggaag agatcgccct
     4261 tgaccgtact catccatgtc cgcccgtcct caagcagaac gtcggaaccg tcgctctcag
     4321 cccaccagcc cacgctaaag ggcttgctct gaccgtagtc gaacgatcga tcgatgcgcc
     4381 atccaatcgg gatcttgaac atgcgcatct tgtggaggtc gttgtcccac acatcgtcga
     4441 acatacctcc cgcaagcacg tcccagctac ccatcaacca agccttgcgc ttgttggcgt
     4501 ctttcatggt gacgagctcg gcgatgtatt ccggactcag gtacttgttc tcgcggtacg
     4561 aaccgaacag atggacttga gtcttgacga cctcttcttg acgctgggtt cgcgggttga
     4621 acacagtcac gcagttccga acgatctcgc ccatcttacc agcatcgatg aatcgcttct
     4681 tgacccaatt gtgcccagcg ccgaacggat tgcaagtggc aaacacttcc agcggaatgg
     4741 gcgggggcgg aggaatgggt tcgccgtcgt catcccaggc tcgatactct tcgggaagga
     4801 aggaagaacg gttgcacgac atcatcatgt cgaacaaggt atccgacgaa tacttggtga
     4861 gctcgttcca tccgatgaat gggaactcct gaccgtgata agcccaataa tccgagtcac
     4921 gacgaatggc gcgaaacaga agctcttcgc ctgacggcca gacccagcgg taatcagact
     4981 tggagctcag gaagcgcgcg ccatccttga actctgggaa ccaccgcatc gacttggaaa
     5041 tcaagtcgtc aaggttcttg tattcgcgat cgaagatcac cccgcgccag tgccgtccgt
     5101 agccctggcc taccctgcgc cggaagcgca tgagctgaga gtcagtcttg cccgggccac
     5161 gggtgccgtg gtaaagcagg atatgagcgg ggcaacagag ggcaagcgtc tgcgagcccg
     5221 gaagcgggct ccatacaacc cgcggagggg cctccactag actcacgtct gagtgtcctt
     5281 gacgagctgc gtctggctat ccttggcagc cttctcccaa tcttcgagag acgcgatacc
     5341 cggcacggcc atcacacctc cgcgatgcag atgctcgttc ttggtcttct ggactccatc
     5401 catgtccaag aagttgccta gcttagccag ggccgccaca cgcgccgcat ggctgctacc
     5461 cgggccacgg tagttggcct cgcgcgtgag actcgccaga atgaaccgct tacgctgctc
     5521 ctcctcagcc ttcgggtctt ccgccgcagc tcgctccagg cgtgcaattt cactctggac
     5581 atacggctcc tccaggaagc gcttggcgta ctcgattgcc atcgtgctca tgaagccagc
     5641 acgcagcgaa gcgccgaagg cgtcgtaatc cttcagatac tccacgatga acttgtcgcg
     5701 aagcacgcgc tcagacgccg tgagctgcgg agccatcgcg tccgctgccc agaagctctg
     5761 ctgtgccgtg ctctcggaca ttgccgcagt cccgtgatgg atttaacgat ccgaagtata
     5821 cgggatcaac gaggactacg caatagatta ttgcccatct gcctttggtt gcagttcacg
     5881 gatctttcgc atgcgctgcg tgcaatcctc cagagctact cgccttgcgc gagcgatccg
     5941 aacagcttcg gatactgtcc gattgagagg aagcgagatc ggacagtcct ccgtcaacgc
     6001 cgagtccaga ggaacgtact tggtcacggt aaccgtgatc gtctgaggaa cgatcggttt
     6061 cgttgcctcg cgacaacctc ccagcatgac cacagcaagg agggtcaata gtccatgact
     6121 gcgggacata gcaattcctt cagtgcgcca cactcgggct tgattttcag agcgtccaat
     6181 tgcttggtcc acaggtcgag atctttcttt ctcagactct gttcctttgc aaggtcttga
     6241 acggcagcgg ccgcttgctg tcggagcttg tccgattcgg ctttgtctgc tttcgacttg
     6301 gaagagatcg tttcgagctt cgcagcaaga actccgatct gcgtgttggc agtatctcgt
     6361 tccccgttgg ccttgtcgat aagcacctta tcggctttgg cttgggtagc ttgaccgcga
     6421 tcgttaccgt ggtgatcgcc ccatccccac aaggaaatgg agataagaaa gatcaatccc
     6481 agcttccaga gccatgcgct agtcagggtc atttgagctt tcctagagcc ttcttgttgg
     6541 agtcggtggc tttgtcggcc gcctgtgctg cttgctgtgc cgtttgcgcg gcctgggtgg
     6601 ctatggtagc ggcccccacc gcttgcgcgc tggctgtagc ggcctgggta gccgtttgcg
     6661 cagtgctacg cgcgagcaat tcgatcgtct gctccttagc ggagatcacc ctctcatact
     6721 gctggcgaat gtcttccaca cgacgatcgc actcgtcgga attcgtgatg tcgttcagca
     6781 tgtagccggc gccgaacacc gcgatcatga caacggtgaa cgcgatccag gcttcgtgct
     6841 tgtgccacca gtcaaagact tttgtcacgt catgactcct gcggtagcag ggttctgcgc
     6901 cttcactgtc tgctcaaggc ttgacacctt cagcgtaaga tccgtgatct tctgctcgag
     6961 ctgttggatg gtctgcatcg ccgcattccg cgcagcctcc gccttgtctg cacgctcgct
     7021 ttctttcgca agctgatcac ggagaaggtt gatgatctcc gtctgcgctg cgttggcaga
     7081 attgatcacg ctgtcgttgg acaggaaccg ccggagccac agaacgcctc cgacaataac
     7141 agcgccgacc attccgagcc agccacttgc ggggatctgc ggcaagacag ctgaaggatc
     7201 gtcaatcatg agtgcttccg cagtggccat aatcttgtcc tgaagtttat tgcctcaggc
     7261 cctatgcagg acccatgccc tgtgatcgcg atagtaactg atcttcgcgc atcgcgctac
     7321 agatttctca cacatccgac agccataccc attttgtgca tcttgtcgat acacgctgtg
     7381 cattccgcct tggtgatctt cccgtcgcgg ttcagatcga aacccgcgtt ctggcgatat
     7441 accgcgccac cgcttgaaat cactgtgtct tcgggaaggc cgatgtattt cggatagaag
     7501 atggccatat acatatccga gcgcgtcttg atgcgcttcg catacggctc gaaatacttc
     7561 ttgacgtagt agagctggct ttcggcagac aagagttcga gattctcgat tgtcgtaccc
     7621 aaacccttgg cggtagcgtc catgaactgg ataagacccg tggccccgct acccgcaccg
     7681 ttcttgatgc tgggactgaa tgtctgtgca gtctcccacg ccatgcaatc catgaggtcg
     7741 ctcgcctggc gaggtgtttc ccatccgaac ccttgcgaga tagaaatcac ctgcgcgcag
     7801 aattcaagac ttactttgtt tccccaggcc aattgcacga ccttctccga agaccaagct
     7861 accggcaaca atacacccgt cctcggtgcg ccgcaaaccc gcatagctcg acccgcccca
     7921 ccagatccaa tggccgtcgg attgatactc gatcaccgct ctgtgtttca agaagcggat
     7981 ccgatacttc ttgccgtgag ccgtgcagta cagcgtatcg ggacgccaac caagacccat
     8041 cagaagtctc atgaaatcct cctgcactat tgctgctcca tcacttggat catctccgcc
     8101 tggacgtcaa gcagcgcatc gaccgtcgca tggatctcct cgcgaagttc ggtttcacgc
     8161 ctcgagtcgt gctggaggac cgctgccatg tgcaactggt tcaacccagc gaacgtggct
     8221 tggaccgcct ggcttcgcga ggtcagctcg aggactttgg cttgcaggct catgaccgta
     8281 tggtctcccc agcagagatc ttggcatcga cccaatgata gaaccattgg aggaacggtt
     8341 cccactccac ttcgcaacgg accttcatgc tgcgctgtgt acctgtggcg gagaagagga
     8401 cgagctccgc gtacatgacc acacgccact tcgagcggtt cacgcgatac acgaggacgg
     8461 gaatctcgcc gttgcggttc gccgatgctt cgcactgcgc ccaccacgta ttcactgcaa
     8521 gctgttcctg gcgcttgact tcaacgctca agccgaacac gttggacagg tctccaccgc
     8581 ctaccgcgct ctggttctgg ttgcgctgca cgacgtcctt ttcgggaacc gggatgctgc
     8641 gcgctttcaa gacggcgttg atgatactgt tgagcgaacg cgctacttct cgctcaccct
     8701 cggcaccctt ctgacggatg ttgattccca tcttacgctt ctccctttgc cgaagcgcgc
     8761 cgctgcaacc tgccgatcgc agaccgggtg tgcccttcgc aggtacgcaa gcgaacgatg
     8821 tgggaatcga ttcttcgcgc gacaccgtgc gcgtcagcag actctgcttc gtctgccgct
     8881 tccttcagaa cgcgttgcgc gtccttcagc ttgttgatga cgacttccag tttctctgat
     8941 ctcgtgaaaa aactcattcc gggttctcac aggcgtgttt ggccagctcc gccatctttg
     9001 caggcggaac gcggttgtcg tcgttcatgt acgcgtaagt aatgccgatc cgataagcct
     9061 tgcgcgtctc tttgtctttg ctcttcgccg cgatctccat cgcctgcgct ttaggcatcc
     9121 ctgcccgttt cttcgcttcc gcgtccatgg tgaaatgact tactgccata cagaagctca
     9181 tcacggtcgc gaacataaga atcctccaat ttgtccatgt agcgctccag gcgaatctcg
     9241 acccacaacg caatcagaag gaacag
//
LOCUS       DATMTI010000343         1893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_310618, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000343 DATMTI010000000
VERSION     DATMTI010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310618"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VN039_05720"
     CDS             <1..362
                     /locus_tag="VN039_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45513.1"
                     /translation="MKAVWYEAIDLMKSGTFPTKKALAEHYYKAESWAERLVREMVSQ
                     SVFTKEQIREIFPQASGAFPGEGQRSPKPVKPQVEKQQTKETVEETAKSDSSLPKSTE
                     KPRRIVYLRGSNKPLHL"
     gene            382..936
                     /locus_tag="VN039_05725"
     CDS             382..936
                     /locus_tag="VN039_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45514.1"
                     /translation="MSNQHRIELDPNTDWNREPTAEELANAPELSQAEMLAVMKSQEY
                     RNSKLVQKLVAASLAKGQGARLQGEEPMQTGDEIAAKQASLAALFRDPRYRTDAAYRY
                     EVSQKIKALTANDNAAISNDDIGKPNQSLSVGLSTSPYHGADLRVRKFARVQIEPTMT
                     GPTAPVKPVAKQSPAKQTPEEFVG"
BASE COUNT          529 a          519 c          523 g          322 t
ORIGIN      
        1 caatgaaggc ggtatggtac gaggcaatcg acctgatgaa gtcggggacg ttcccgacaa
       61 agaaagcctt agccgagcac tactacaagg cggaaagctg ggccgagcgc ttggtacggg
      121 agatggtcag ccagagtgtc tttaccaagg aacagatccg agagatcttt ccgcaagctt
      181 ccggagcctt cccaggcgaa ggccagcgaa gccctaagcc agtcaagccc caagtagaga
      241 agcagcaaac caaagagaca gttgaagaga cagccaagag cgacagctct ttgcccaaga
      301 gcaccgagaa gccaaggaga atagtctatc tccgtggctc caataaaccc ctacaccttt
      361 aacctataag gaattaccac tatgtccaac caacaccgaa ttgaactaga cccgaacacc
      421 gattggaacc gtgagccgac cgccgaagaa ttagccaatg cccccgaact ctctcaagcc
      481 gagatgctcg cagtgatgaa gagccaagag taccgcaata gtaagcttgt acaaaagctt
      541 gttgccgctt ccctggcgaa aggccaaggg gcacgacttc agggtgaaga gccgatgcaa
      601 acaggtgacg agatcgcggc aaagcaagcc tcactggccg cccttttccg agatccccgc
      661 taccgcacag atgcagccta ccgttatgaa gtgagccaga agatcaaggc gctcacggcg
      721 aacgacaacg cggcaatcag taacgacgat atcgggaaac cgaatcagtc cctctccgtg
      781 ggcctgagca ccagtcccta ccatggagca gacctgaggg ttcgcaaatt cgcgagggtg
      841 cagattgagc cgacgatgac ggggccgacc gctccagtga agccggtagc caaacaatcc
      901 cccgcgaagc agacgccgga agagtttgta ggttaagggg tggtacccat cgaagacgag
      961 catcacttga gatatgaggt gaaggtctac cgctgcatcc aaggccatgc tgtaccgact
     1021 cactcaaccg gtgagcatgg cgtctgtgtc tgcggatggg agttgtggtc aaggaacggt
     1081 agggctgaaa tccttgaagc ccatggccac catgttgcat tcgaacgccg gaagagagaa
     1141 gcagaacaaa ccatgaacca tgggctgcaa ctgaacgtaa ccggagatgc tgccacgtgt
     1201 tcgtattgca attggttcta ttcgtctacc tcgattgcca aaataatgaa tgagtttcat
     1261 caacaccaga aggacgtgag aaatgcagtg tagcaacaac aacaacgaaa gcccaggtga
     1321 gcaccgccac caccagagac ggagagaaca acaacaaggg atcgcttggg gttattggtc
     1381 cggtcctcag cggttcacgg tagagacctg ccccacttgc aagatcaaca agattctact
     1441 ggacggagag acctggcacc acgactgcca ggcaccaccc catatcattg tctggatcac
     1501 cccggttcat gcggcttgta ccggttgcga ttggcggcat ggggcgaagg gaagcatgag
     1561 ccggggcgga gtcagggaag tgtacaggaa ggctcacctc ccccttcagt aagaacacat
     1621 acaaccacag tctagctagg cagcgggtgc ccttaccggc aacgatgggg acagactcac
     1681 tctgctttca acacccgttg ccttaatcct tgaaagggat gtgaccgatg gtattacgta
     1741 aggaacagat caacactctc acaacccaag cgcaccagtg gcacatggcc accttggaac
     1801 gctacgggaa caagccgaac gaaatccagc gcaccattct gcgggaactc atgcggatgt
     1861 tcgcagggat ggccgctggg caaacggttg ggc
//
LOCUS       DATMTI010000344         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_183212, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000344 DATMTI010000000
VERSION     DATMTI010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183212"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VN039_05730"
     CDS             complement(<1..1199)
                     /locus_tag="VN039_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV45515.1"
                     /translation="MASSRQRVFIEAVPMVALTATICVLDVLTSLAWSVWLLYFIPLA
                     LVLHRSPNPVPYQFAALATFLTMLGGYLSPPDIHPDVALFNRCLGMAVMWGFTWYTVK
                     QKPVQASLDTETARSHAAPAATVAGRPKVEEQLLISRLRLESIIESAMDAIITVDEDQ
                     KIVLFNHAAEQMFGCSTREAIGRTLDRFLPERFREAHRHHIHTFGQSGVTSRKMGKLG
                     IVMGLRSNDEEFPIEAAISHIALDGKKYYTVILRDISERKRAEDLLQESQRQLTTLIG
                     NLPGFVYRCRNDRGWIFEYLSDGVSDLTGYPAEEYLVQRTISYGQNTHPDDRERVRHE
                     IQAALEQHRSFELTYRILTKAGEVKWVWERGEGIYAQDGTLSYLEGFVTDVTERKRAE
                     RLLRQSE"
     gene            complement(1204..2937)
                     /locus_tag="VN039_05735"
     CDS             complement(1204..2937)
                     /locus_tag="VN039_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45516.1"
                     /translation="MSLFRKPRAPDDDLPELCHSLMERAPAPMAELEGIEHILRYVNP
                     AFCRLVGKDRTALIGRSFAETAQDGDCCLSVLDRVYHTAEPETYTEVEGAESHPLYWS
                     YAVWPVVGVDQQPVGVMMQVTETTRFHQQTAAMNQELLRSAVRHHELTEVAEKLNDEL
                     ERRVSDRTQELEQSQGRLRALATELNLAEQRERKRLATDLHDYLAQLLVLGRMMLGQA
                     KRSGLPTRGEDFVKEAEEALDKALTYCRTLMAELKPPVLQERGFSAGLIWLGDHMKRQ
                     ELTVSIDIPEAFDLPLPEDRAVLLFQSVRELLINVAKHGTVKQASVRMTYENGLLKII
                     VQDENGFDLAAATTTTTTTTSDNTSLLSSKFGLFSIRERMKALGGDFEIQSAPGEGTT
                     ATLSLPVGTRTEDSGLKTESSEKPVGRQPSLISSPSDRSVLSTQSSALQKNAPIRVLL
                     VDDHAVLRQGLRSIVAAYAQLEVVGEAGDGIEAVTLTQQLGPDVVVMDINMPNMDGIE
                     ATRRIKENRPDTIVIALSVNQSADTERKMRAAGAFTYLRKESAADALRQAIEEAMASK
                     QETAAHNTAPD"
BASE COUNT          621 a          887 c          847 g          700 t
ORIGIN      
        1 tcttcgcttt gccgcagcaa gcgttcggct cgcttgcgtt cggtcacgtc ggtcacgaaa
       61 ccttccaagt aactgagggt gccgtcctgc gcatagattc cctcgcctct ctcccaaacc
      121 catttgacct cgccggcttt cgtaagaatg cgataggtca actcgaagga gcgatgttgc
      181 tccaacgcgg cctgaatttc atgtcggact cgttcacgat catccggatg ggtattttgt
      241 ccataagaaa tcgttcgttg tacgaggtat tcttctgcgg ggtaaccggt cagatccgac
      301 actccatcac tgaggtactc gaatatccaa ccgcggtcat ttcgacaacg atacacgaag
      361 cccggcagat tgccgattaa ggtagtgagt tgccgctggc tctcttgaag caagtcttca
      421 gcccgttttc gctcggagat gtctcgaaga atgacggtat agtatttctt cccgtccaga
      481 gcgatatgcg aaatggcggc ttcgatcgga aactcttcgt cgttcgagcg gagccccatg
      541 actattccca gtttacccat ctttcggctc gtcacgcccg attggccgaa ggtatgaatg
      601 tggtgacgat gggcctcgcg gaaccgctcc ggcaagaatc tgtcgagcgt ccgcccgatt
      661 gcttctcgag tggaacagcc gaacatctgc tccgccgcat ggttaaacaa cacgattttc
      721 tgatcttcat ccacggtgat gatcgcatcc atcgcggatt caataatgct ttccagcctg
      781 agccggctga tcaggagctg ctcttccacc ttcggacgcc cggctacggt agctgccgga
      841 gccgcatgcg atcgagccgt ttctgtgtca agggaagctt ggaccggctt ctgcttcacg
      901 gtgtaccaag tgaatcccca catgacggcc atccccaaac agcgattgaa tagggctacg
      961 tcgggatgaa tatcgggggg ggataggtac ccgccaagca tcgtgaggaa ggtcgcgagc
     1021 gccgcgaact gataagggac cggattcggc gaacggtgca gaacgagcgc gagtgggatg
     1081 aagtacaaca gccagacgga ccaggcgaga gaggtgagta cgtccagaac acaaatcgtg
     1141 gccgtgaggg cgaccatggg gacggcttcg atgaaaaccc gttgccggct cgatgccatc
     1201 acatcaatcc ggcgcagtgt tgtgcgcggc cgtttcctgc ttagaggcca ttgcctcttc
     1261 gatggcctga cgcagggcat cagccgcact ttcctttctc aggtacgtga aggcaccggc
     1321 agctctcatc ttccgctccg tgtcggccga ttgattcacc gagagggcga tgacgatggt
     1381 gtccggccgg ttttctttga tccgtctcgt cgcttcaatt ccgtccatgt tgggcatgtt
     1441 gatatccatc accacgacat cgggaccgag ctgctgggtg agtgtcacgg cttcgattcc
     1501 gtcgcccgct tcgccgacga cctctaattg agcataggcc gctacaatgc tccgcaagcc
     1561 ctgccgcaac accgcgtgat cgtccacgag aagcacgcga atgggcgcgt tcttctgaag
     1621 tgctgaggac tgagtactga ggactgagcg atcggaagga gagctgataa gtgatggctg
     1681 acgaccaaca ggtttttccg aggactcagt cttcagtcct gagtcctccg tcctcgttcc
     1741 caccggcaag ctgagcgtcg ccgtggtgcc ctctccgggc gccgattgga tctcaaagtc
     1801 accgcccagc gctttcatcc gttctcgtat actgaacagg ccgaacttcg aagagagcaa
     1861 tgaggtatta tcagaggtag tagtagtagt agtagtagta gcagcagcaa gatcaaagcc
     1921 attctcatcc tgcacgataa tcttcagcaa tccattttca taggtcatcc gcaccgacgc
     1981 ctgtttgacc gttccatgtt tcgccacatt gatcaacagc tctcggaccg attgaaacag
     2041 cagcaccgcg cggtcttcag gcaacggcag gtcgaaggct tctgggatgt cgatgctgac
     2101 cgtcagttcc tgccgtttca tatgatcgcc cagccagatg agcccggcgg agaaacctcg
     2161 ttcttgcaac accggaggtt tcagctcagc catcaacgtc cggcaatagg tcaaggcctt
     2221 gtctaaagcc tcttcggcct ctttcacgaa gtcttcgccg cgcgtgggca agccgctccg
     2281 cttcgcttgg ccaagcatca tccgaccgag gacgagcaat tgggccaggt agtcatggag
     2341 gtcggtcgcc aatctcttcc gttcgcgctg ctccgcaagg ttcagctccg tggccaatgc
     2401 gcggaggcga ccctgtgact gctccaactc ttgggtgcga tcggatacac gccgctccaa
     2461 ttcatcgttc agcttctccg cgacctcggt gagttcatga tggcgtaccg cggagcgcag
     2521 caactcctga ttcatcgcgg ctgtctgttg atggaaccgc gtcgtctcgg tgacttgcat
     2581 catgactccc accggctgtt gatcgacgcc gacaactggc caaacagcat acgaccaata
     2641 gagcggatga gactcggcac cttccacttc ggtataagtt tccggttcgg cggtgtggta
     2701 cacgcggtct agtacggaca ggcaacagtc tccatcctgg gcggtttcgg cgaaggacct
     2761 cccgatcaat gccgttcgat ctttgccgac cagacgacaa aaagccggat tgacatagcg
     2821 caggatgtgc tcaatgcctt ctaattccgc catcggcgcc ggcgcgcgtt ccataaggga
     2881 gtggcagagt tccggcagat cgtcgtccgg cgcgcgtggt tttctgaata aggacatttc
     2941 tatggctctt ccgatcggtc tggtcttttc cagtatgtcg ggatgccggt gatcagcgcg
     3001 cgatactcat tcaggcgcgt ctcggtcagg accactccct tcttggtgat ctcgt
//
LOCUS       DATMTI010000345         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_267186, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000345 DATMTI010000000
VERSION     DATMTI010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267186"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1716
                     /locus_tag="VN039_05740"
     CDS             166..1716
                     /locus_tag="VN039_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HWV45517.1"
                     /translation="MSKLAVIDIGTNSIHMVLAEILPDAGFKILDRFKDMTRLGNGVF
                     ATRLLSDEAMNRALEVLKTLVTLARNKGFERIVAVATSAVREAQNGGDFVALILEQTG
                     LRVRVISGTEEARLIFLGVKHSIALPDGPTLVVDIGGGSVELIVGNREGLIHCKSLKL
                     GAIRLAEQFLPKTPPSESMLQALDDAVLTHLREALGSFKMKKFHSLVATSGMAGNLGE
                     VVHLRQTGRPLPQHNLATVLSKDIRSLETELARSSVKARLAIPGLDPKRVDTLLPTAL
                     VLRRLLDLSGLSEVILCDKAIREGVIYDFIVRHREGLKAEHDIPDVRRRNVIGLARRC
                     QAPEVHSLHVADLALSLFDQTKKEHRLGQQERTWLEYAAILHDVGYLINLRQHHKHAY
                     YLIKHSDLGGLTAEEIDVIANIARYHRRALPSLKHETFNCLTPRLQRVVKILASLLRI
                     ADGLDRTHFSLVRAVNVKLGKQMTIEVRLTGDAEMELWAAKGRADLFEQVFRRRALFS
                     GVLETEPS"
     gene            1713..>1850
                     /locus_tag="VN039_05745"
     CDS             1713..>1850
                     /locus_tag="VN039_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45518.1"
                     /translation="MTEPHILKATPHPHPGKLIIVEGIDGSGKSTQLQLLHKWLESKG
                     HK"
BASE COUNT          417 a          526 c          505 g          402 t
ORIGIN      
        1 gagaaaccga agcgtccatt tcatcgttca ataatgctcg aatcctactc cgccctactc
       61 ccccggctgt caggtatatg tcacgcacag tttacatgct gattgttgac aggccgcccg
      121 tactttcatt aaggtccatt gattctccac tcctagggaa cttttgtgtc taaactcgcc
      181 gtcatcgaca tcggaacgaa ctctattcat atggtgctgg ctgaaatact tcccgatgcc
      241 ggattcaaga tactcgaccg ttttaaggat atgacgcgcc tgggcaacgg cgtattcgca
      301 accaggctgc tctcggacga ggccatgaac cgtgcgctgg aagtcctcaa gacgctggtc
      361 acgctcgccc gcaacaaagg attcgaacgt atcgtcgccg tcgcgaccag tgccgtgcgc
      421 gaagcccaaa acggcggtga tttcgtcgcc ttgatcctgg agcaaacggg gctgagggtg
      481 cgggtgatca gcggaacaga ggaagctcgg ctgatcttcc tgggcgtcaa gcacagtatc
      541 gccctcccgg acggaccgac cctggtcgtc gacatcgggg gcggctccgt ggagttgatc
      601 gtggggaatc gagaaggcct gatccactgc aagagcctca agcttggagc gatccgttta
      661 gccgagcaat tccttcctaa aactccaccg tctgagtcga tgctgcaggc cctggacgat
      721 gccgtgctca cccacctgcg cgaggcgctc ggatcattca agatgaagaa gtttcattca
      781 ctggtcgcga cctccggtat ggcaggaaac ctcggtgagg tcgtccacct ccgtcaaacc
      841 ggacgaccgt tgccgcagca caatcttgcg accgttttat cgaaggatat ccgcagcctc
      901 gaaacggagt tggctcggtc atcggtgaag gctcgtttgg cgattcccgg tctggacccc
      961 aagcgggtcg atacgctgtt gccgacggcc ctcgtcctcc gacgtctttt ggatctgtcc
     1021 ggtctcagcg aggtcatact ctgcgacaag gctattcgtg agggtgtcat ctacgatttt
     1081 atcgtacggc atcgagaggg tctgaaagcc gaacatgata ttcccgacgt tcgacgtcgc
     1141 aacgtgatcg gcctagcccg acgctgccag gcaccggagg ttcattcact ccatgtggcc
     1201 gacttggcac tgagtttgtt cgatcaaacc aagaaagaac atcgcttggg acaacaggag
     1261 cgaacctggc ttgagtatgc agccattctg cacgatgtcg gatatctcat caatctgaga
     1321 caacatcaca aacacgcgta ctacctcatc aagcatagcg acttgggcgg attgacggct
     1381 gaagaaatcg atgtgatcgc caacatcgcc cgctatcacc ggagagcgtt gccgtcgctg
     1441 aagcacgaaa cattcaactg tctgacaccg cgtttgcagc gtgtggtcaa gatccttgcc
     1501 tcattgctgc ggatcgccga cgggcttgat cggacccact tctcgctcgt tcgggccgtg
     1561 aacgtcaagt tggggaaaca gatgaccatt gaagttcgtt tgacagggga cgcggagatg
     1621 gaattatggg ctgcaaaggg cagagccgac ctgttcgaac aagttttccg tcgacgtgcc
     1681 ctattctccg gagtgctgga aaccgagcca tcatgaccga acctcacata ctcaaagcaa
     1741 cccctcaccc tcacccgggg aagctgatca ttgtcgaagg catcgatggg tcaggcaaga
     1801 gtactcaatt gcaactgctg cataaatggc ttgagtccaa gggacacaag
//
LOCUS       DATMTI010000346         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_480252, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000346 DATMTI010000000
VERSION     DATMTI010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480252"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VN039_05750"
     CDS             complement(<1..458)
                     /locus_tag="VN039_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWV45519.1"
                     /translation="METRSCLFCGAPLRRTFVDLGMHPLCESYVSEAKLNAMEPFYPL
                     HVYVCERCFLVQLHEYVSPADIFTEYAYFSSYADSWVQHAKRYTEMIEERLKLTAKSL
                     VVELASNDGYLLQHFVAKGIPVLGIEPAANVAEAARKKNVPTLVKFFGRNT"
     gene            complement(493..1695)
                     /locus_tag="VN039_05755"
     CDS             complement(493..1695)
                     /locus_tag="VN039_05755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWV45520.1"
                     /translation="MTKENIPFLDLVAVHQELQDEFVEVLKTALKSAGFIGGPMVQGF
                     EEDFSRFCETRFCVGVGSGTDALRFALIAAGVKSGDIVLTVPNTFIATTEAVSQAGAQ
                     PDFVDIDPKTYAMDPGKLREYLEQKCRWNTKTRQVIHNATGKPITAVVPVHLYGQMAD
                     MDPILDICAHYNLKVIEDACQAQGAEYFSRKDNRWHKAGSMGHAAAFSFYPGKNLGAC
                     GEAGAVTTNDDEIAQTCRLLRDHGQSKKYFHDIEGYNGRLDAIQAGFLRVKLHHLSKW
                     NEQRRQVAERYDKLFAGAEGIVTLPHQPSWARSVYHLYVVQVEDRARLQQQLSDVGVG
                     TGIHYPVALHLAKAYEGLGFQLGGFPIAEKSAAHILSLPMFPELTLDQQERVVKEVLK
                     SLSVNNES"
     gene            complement(1688..>2020)
                     /locus_tag="VN039_05760"
     CDS             complement(1688..>2020)
                     /locus_tag="VN039_05760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWV45521.1"
                     /translation="SSHTFICEGVTIEDEVFIGHNVTFVNDTFPRATTTTGSLQTEAD
                     WKVETTLVKRGASIGSGATILANVVIGEHALIGAGAVVTKDVPAHAVVAGNPGKVVRY
                     VKSGGSHD"
BASE COUNT          443 a          592 c          516 g          469 t
ORIGIN      
        1 gtgttccgac cgaagaactt gactaacgtc ggaacatttt ttttcctcgc cgcctcggcg
       61 acattggctg caggctcgat tcccaagacc ggaatgccct tggcgacaaa atgctgcaac
      121 aggtagccgt cattactggc aagttcgacc accaaactct tcgccgtcaa cttgagccgc
      181 tcttcgatca tttccgtata tcgctttgca tgctgcaccc aactgtccgc gtaagaagaa
      241 aagtatgcgt actccgtgaa aatgtccgcc ggactgacgt actcgtgcag ttgcacgaga
      301 aaacagcgtt cgcagacgta cacgtggaga gggtaaaagg gttccattgc gttcagtttg
      361 gcttcgctca cgtaactttc gcagagagga tgcatcccaa gatccacgaa ggttcttcgc
      421 aatggcgcgc cgcagaacaa acaacttctg gtttccatat agccgatcct tccttgcaga
      481 tggcacccgg atctatgact cgttgttgac agagagagac ttcagaactt ccttcactac
      541 cctctcctgc tggtccaagg tgagttcagg gaacatcgga agcgacagga tatgcgccgc
      601 tgatttctcg gcgatcggaa agcccccgag ctgaaatccg agcccttcgt aagcttttgc
      661 cagatgcagc gctaccggat aatgaatccc cgtcccgacg cccacgtcgc tcagctgctg
      721 ttgaagccga gcacggtcct cgacctggac aacatagagg tgatagaccg accgcgccca
      781 gctcggctga tgcggcaacg tcacgatccc ttcggctcca gcgaacaatt tgtcgtaacg
      841 ctccgccacc tgtcggcgct gttcgttcca tttcgacaaa tggtgtagtt tgacgcgcaa
      901 gaagccggcc tggatggcat caaggcggcc attatagcct tcaatatcgt ggaaatattt
      961 ctttgattgc ccatggtcgc gcaagagtcg gcatgtctgc gcgatctcgt cgtcattggt
     1021 cgtgaccgcc cccgcttcgc cgcaggcgcc gagatttttg ccgggataaa agctgaacgc
     1081 ggcggcgtgt cccatcgagc cggccttgtg ccagcgatta tccttccgtg agaagtattc
     1141 agctccctgc gcctgacatg cgtcttcaat gacctttagg ttatagtggg cgcaaatatc
     1201 caaaatcgga tccatgtcag ccatctgccc atagagatgg acgggaacga cggccgtaat
     1261 cggcttaccg gtcgcattgt gaatgacttg tcttgtcttc gtattccacc tgcacttctg
     1321 ctccagatac tcacgcaatt ttccgggatc cattgcatac gtttttggat cgatgtcgac
     1381 gaaatccggt tgagcacccg cttgggaaac ggcttcggtc gtggcaataa acgtgttggg
     1441 aaccgtgaga acgatgtcac cggacttcac accggcggca atcaaggcga acctaagagc
     1501 gtctgtaccg ctgccgacgc cgacacagaa tcgggtctcg cagaaccgag aaaagtcctc
     1561 ttcgaatcct tgcaccatcg gtccaccaat aaatcccgcg ctcttcaggg cagtcttcag
     1621 cacttcgacg aattcatctt gcaactcctg atgaaccgcg accagatcta gaaaggggat
     1681 gttctcttta gtcatggctg cctcctgact tgacatatcg caccaccttg cctggatttc
     1741 ccgcgacgac ggcatgtgca ggcacatcct tcgtgacgac cgccccggct ccgatcagcg
     1801 cgtgctcccc gatcaccaca ttggcaagaa ttgtcgcgcc ggacccgatc gatgcgccac
     1861 gcttcaccaa tgttgtttcg actttccaat ccgcctcggt ctgtagtgag ccagtcgtgg
     1921 tggtcgcgcg aggaaaggta tcgttcacga acgtgacgtt gtgtccgata aagacttcgt
     1981 cttcgatggt cacgccctca cagatgaagg tatggctcga
//
LOCUS       DATMTI010000347         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_437261, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000347 DATMTI010000000
VERSION     DATMTI010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437261"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VN039_05765"
     CDS             <1..1135
                     /locus_tag="VN039_05765"
                     /inference="COORDINATES: protein motif:HMM:NF015154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HWV45522.1"
                     /translation="GRFEIIRQRHTDNTCCQYDINSAYPAAIKDLPCLAHGRWTRVER
                     PTKPVRFGIYRIKANNISDDWTRPLPLWYRTRNGRVVYGNDVHNWYWTPEAEIALEYG
                     LEIEEGWGYNQHCDHKPFWFVPVLFDQRAELKRRKDGAHVGIKLGLNSLYGKLAQQVG
                     WGYNDDGTLRIPPYHCLEWAGYVTSSCRAQLWRAAQLAPDDVIAFETDAIFSRVPLNV
                     DIGSGLGQWEATDYTSLTYLMSGYYFASKPDGEQVEKSRGVNKGSVSRMDAILAIENF
                     AHIDAEQTRFITLGQALHQNFDLWQQWITSPRRLTARIEGKRIHALDDLHGWHESEWL
                     QTWPNPLLPMENGMSKEYDVEWLMNTDYDRSLTRRQDEDRDYE"
     gene            1480..2268
                     /locus_tag="VN039_05770"
     CDS             1480..2268
                     /locus_tag="VN039_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWV45523.1"
                     /translation="MNLTSSSIFAPMLNLFPRKGTAGNKLDIITWNVYVGHKPSDVKT
                     ALTDMAKEYNPHIFALQEATNLGANRIRRTKGRPRTEWGNGRQDPNRAIHLPGYRTYH
                     LPKDHPKGLYGEAADTALLIRNDVIIHGHQYNKMKEKWKGPDHGFIHEPRVYQTIWVS
                     SAITGEVWKIGAGHWPFGSALQETINYVHDWFAKTIKGRPTVWVGDLNAMPHDFKSIT
                     DLSDVKVVGHRIDQAITRNVKMATAKDIGAHGSDHDAVLIHLEA"
     gene            2268..>2478
                     /locus_tag="VN039_05775"
     CDS             2268..>2478
                     /locus_tag="VN039_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45524.1"
                     /translation="MGNYLCEDSSCEWHDKLNRQGACEYADNGATRCPSWRCDCFIET
                     HPDSPMGLHPEDFIVSLGDLSPIPHY"
BASE COUNT          678 a          672 c          618 g          510 t
ORIGIN      
        1 cggacgtttc gagattatcc gacaaagaca tactgacaat acgtgttgcc aatacgacat
       61 taacagcgcg taccctgccg caattaaaga cctcccttgc ctcgcgcatg gcaggtggac
      121 acgcgtggag cgaccaacca agcctgttcg attcggaatc taccgaataa aggctaacaa
      181 tatctccgac gattggacac gccctctccc gctttggtac cgcaccagaa acggaagagt
      241 cgtctacggc aatgacgtac ataattggta ctggactcct gaggcagaaa tagcattgga
      301 gtacggttta gagattgaag agggatgggg atacaaccag cactgtgacc acaaaccatt
      361 ctggtttgtg cccgtcctat tcgatcaaag agcagaactc aagcgccgga aagacggggc
      421 gcatgtagga attaagctcg gtcttaattc gctctatgga aagcttgccc aacaagtagg
      481 gtggggatac aacgatgatg ggaccttgcg aatccctccc tatcactgtc tcgaatgggc
      541 cggatatgtt acgtcgtcat gccgcgcgca actctggcgc gctgcacagc tagcacctga
      601 tgatgtgatt gcatttgaga ctgacgctat tttctcccgc gtccctctca atgtggatat
      661 cggctccggt ctagggcaat gggaagcgac ggactatacg tcacttacct atctcatgtc
      721 aggttactat ttcgcttcta agcctgacgg ggaacaggta gagaaatcgc ggggagtaaa
      781 caaaggctct gtctcgcgta tggacgcaat tctagctatc gagaatttcg cccatataga
      841 cgccgagcag acgcgcttta ttaccctcgg tcaagccctc caccaaaact ttgacctctg
      901 gcagcaatgg ataacgtccc cgcgccgact cacggcacgc attgagggaa agcgtattca
      961 cgcgctagat gaccttcacg gttggcacga atccgaatgg ctacagacgt ggccgaatcc
     1021 tctattgcca atggaaaatg gcatgtctaa ggaatacgat gttgagtggc ttatgaatac
     1081 cgactatgac cgttcgctaa cacgtcggca agatgaggat agagactatg agtaaaccta
     1141 agtatattcc acttgaccta gaatgcgggc ataggctctg gttccagcgt acacatatgc
     1201 cccgaaagca tgatcgtatt tggtgccgct cttgtcagga aaacgttcgg tctttacgat
     1261 cggttaaaga cgacggcatg accatatggg aggggtacta tacccttgcc aataagatcg
     1321 gagttatggt aggatgcaac catgaaaccg aatgcaattg gacagcacaa gctagaacct
     1381 tgcaacacgc cgaacagcta gcatttagcc acatcgcgcg cgaacacgga aaatccacgc
     1441 tactcaatac cgaaaccctt acgctaaagg aaatcccaag tgaacctgac ttctagcagc
     1501 atcttcgccc cgatgcttaa ccttttcccg cgcaagggca cagcgggtaa caagcttgat
     1561 atcattacgt ggaacgtcta cgtaggacac aagcctagcg acgtcaagac cgcgcttacc
     1621 gacatggcaa aggaatacaa cccccacatc ttcgcactac aggaagcgac aaacctcgga
     1681 gctaaccgca tccgtcgaac aaaaggtcga ccgcgtactg aatggggaaa tggaaggcaa
     1741 gatccaaacc gtgctatcca ccttcccgga taccgcacct accatcttcc caaggatcac
     1801 cccaagggac tatacgggga agcggcagac acggcactac tcatccgtaa tgacgtgatt
     1861 atccacggac accaatacaa caaaatgaaa gaaaagtgga agggacccga tcacggattt
     1921 atccatgagc caagggtcta ccaaaccatt tgggtatcca gcgccattac cggagaggta
     1981 tggaaaatcg gtgcaggaca ttggccgttc ggctctgccc tgcaagagac aatcaattac
     2041 gtacacgact ggttcgcaaa gaccattaag ggacgcccta ccgtatgggt aggggacctc
     2101 aacgcgatgc cccatgactt caagagcatt acggacctat cagacgtaaa ggtagtcggc
     2161 caccgaatcg accaagccat tacccgcaac gttaaaatgg cgacagccaa ggacattggc
     2221 gcgcatggct ccgaccatga cgccgtactc attcacttgg aagcataatg ggtaactacc
     2281 tctgtgaaga tagctcgtgt gaatggcacg acaaacttaa ccgtcaaggt gcgtgcgagt
     2341 acgccgacaa cggggcgact cgctgtccgt cgtggcgctg cgactgcttt atcgagacgc
     2401 accctgacag cccgatgggc ctgcacccgg aggacttcat cgtgtcgctc ggggatttgt
     2461 cacctatccc tcactacc
//
LOCUS       DATMTI010000348         6427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_691845, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000348 DATMTI010000000
VERSION     DATMTI010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691845"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /gene="atpD"
                     /locus_tag="VN039_05780"
     CDS             <1..1458
                     /gene="atpD"
                     /locus_tag="VN039_05780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWV45525.1"
                     /translation="GRSPTVSTGKVIQVIGTGVDVEFPPGRLPNIYNAIKVTQEENKA
                     AGTPAIRITLEVASHLGENRVRSIAMSTTDGLTRGMDVQDTGAPISVPVGRETLGRLI
                     NVLGEPVDEKGPLKTKKTYPIHRPAPKLEDQETKTEVLETGIKVVDLLEPYSKGGKVG
                     LFGGAGVGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQESKVIDPDDF
                     TKSKAALVYGQMNEPPGARLRVGLTGLTVAEYFRDEENQDVLLFVDNIFRFTQAGSEV
                     SALLGRMPSAVGYQPNLSTEMGALQERITSTKRGSITSVQAIYVPADDLTDPAPATAF
                     AHLDATTVLSRSLAELGIYPAVDPLDSTSRILDPQIIGEEHYKIARGVQSVLQRYKDL
                     QDIIAILGMDELSEDDKMVVARARKIQRFLSQPFHVAEAFTGAPGKYVKLKDTVRSFR
                     EILDGKYDHLPEQAFYMVGPIEEAVAKAEKMGVKV"
     gene            1588..2010
                     /locus_tag="VN039_05785"
     CDS             1588..2010
                     /locus_tag="VN039_05785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWV45526.1"
                     /translation="MAGKILLEVVTPERQLLSQQVDEVIAPGSEGEFGVLPGHCHFLS
                     TLRIGELRYRVDGQTHSMAVLWSFAEVTPTKVTVMAEVAEKAEDIDVERATAKVTEAE
                     RRLQAGGLPSEVKEAQISLEKARLRKKIAERSQKTGHA"
     gene            complement(2270..2878)
                     /locus_tag="VN039_05790"
     CDS             complement(2270..2878)
                     /locus_tag="VN039_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HWV45527.1"
                     /translation="MTFSQKMLWGAALLGLTIGGAIVLYPARTLPSTTSICLSMDKVA
                     DYIHAVIEADRDSYTRHVVERMQTKGIVVASENWEDMHTLPLPAQFLLESGRHLDKKG
                     IGIQYRLISLWPINKRNVATTPLQSIALGTIVTQPNHPFTGVTKVGDTRYYEAVYPDL
                     AVTQACIGCHNAHPNSPKRDFKVNDVMGAIVISIQLGPGFPG"
     gene            3352..3891
                     /locus_tag="VN039_05795"
     CDS             3352..3891
                     /locus_tag="VN039_05795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV45528.1"
                     /translation="MQFPLEAKLKDETPIELLPAGNRDRLALAKLYDRIVAEGTSYPH
                     EGPVTEDEFLDYWVRGKRTVVAYERPRKTDAKLLGAFYLKPNWPGRARHVANAGFIVM
                     PEWRNRGLGRLLGAVMLDYAEELGYRSVIFNLVFSENQGARHLWEKLGFRVLGAVPGA
                     VRTNDGKYQDALIMFRSLI"
     gene            3904..4689
                     /locus_tag="VN039_05800"
     CDS             3904..4689
                     /locus_tag="VN039_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV45529.1"
                     /translation="MTAQQQWNPQQYAEHARFVTDLGAPLIDMLALKPGERVLDVGCG
                     DGVLTKQLMDRGCDVIGVDASPEMVEAAKLCGVNAQIMDGHRLPFYEEFNAVFSNAAL
                     HWMVRPDEVLHSVRRALRPGGRFVAEFGGRGNLTKLLAGLHKILRAYGCDPAAVQPWY
                     FPTAEEYRAKLEQQGFQLVSIDVFPRPTELPGDITKWLEMFARPFLLLIPSASQAEFL
                     TRAREALRPILATPDGRWTVDYVRLRCHAVKPTAPRFSLSPEI"
     gene            4721..5707
                     /locus_tag="VN039_05805"
     CDS             4721..5707
                     /locus_tag="VN039_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV45530.1"
                     /translation="MTPLHLLVTLVLSAGAWPLDEPWLLQPVSSARTLKGDELLRIGE
                     IHDVQNHFSEALTYYDQARESFRATKQRQGEATALTKIGSVFERQGRREAAAAQLRDA
                     LALFSKVPASPAHGDALLTLGKVSAWLGSREEAGRLFVRAADRYSRSRNIQGAVSARI
                     QLGLLRIGDGLTQEGLQLLEQVLQDAHNRQDNDQTLAALFAVGDARWIMDEPDAAEQY
                     YARALALVEQRPQAGMEAALRYRMSAVYDAIGQQEKGIGSAKRAVTLYQSLRDPSGEA
                     ASWALLASLYEALGKHQEADEAGQRALAIFRRRQVIVHAAHPSANSLLSESR"
     gene            5810..>6427
                     /locus_tag="VN039_05810"
     CDS             5810..>6427
                     /locus_tag="VN039_05810"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWV45531.1"
                     /translation="MITEFVVTSGEQPKRLDLFLVNRERDISRSALQRLIELGRIRIN
                     DQVVKASHKIKPGDRITMDVPKPEPLSIKGEAIPLDILVEDDALLVLNKPSGIVVHPA
                     PGNWTGTLVNALLHHFQTSGGTISTIGGKERPGLVHRLDKETSGIMVITKTDAAHRVL
                     AAQFKLHTIIRVYEALVWGVPKKGRGLIELAIGRDVKDRKKFSSRT"
BASE COUNT         1426 a         1789 c         1874 g         1338 t
ORIGIN      
        1 ggaaggagtc ctacagtgag tacaggcaag gtcattcaag tcatcggaac gggcgtggac
       61 gtggaattcc ctcccggccg gctacccaac atctacaatg cgatcaaggt gacgcaggaa
      121 gagaataagg cggcgggtac acctgccatt cggatcactc ttgaagttgc ttcgcacctg
      181 ggcgaaaacc gagtccgcag catcgcgatg tccacgaccg atggtctgac gcgcgggatg
      241 gatgtgcagg ataccggagc cccgatttcg gtgccggtcg gacgcgaaac cctcggccgc
      301 ctcatcaatg tactcggtga gccggttgac gaaaaggggc ctctcaagac caagaagacc
      361 tatccgatcc accgccctgc tccaaagctg gaagatcaag aaaccaagac cgaggtgctg
      421 gaaaccggta tcaaagtcgt cgatttactc gaaccgtata gtaagggcgg caaggtcgga
      481 ctgttcggcg gcgccggcgt cggtaagacc gtcatcatca tggagctcat caataacatc
      541 gcgttgcacc acggtggatt ttccgtattc gccggcgtcg gcgaacgcac ccgagaaggg
      601 aacgacctgt ggcacgagat gcaggaatcg aaggtcatcg atccggacga tttcaccaag
      661 tccaaggccg cgttggtcta tggtcagatg aatgagccgc ccggcgcgcg acttcgcgtg
      721 ggactgaccg gcctcacggt cgcggaatat ttccgtgacg aagagaatca ggacgtattg
      781 ctcttcgtgg acaatatttt ccggtttact caggcgggtt cggaagtgtc cgcattgctg
      841 ggccgcatgc cttccgccgt cggctatcag cccaacctct cgaccgaaat gggtgccctg
      901 caggaacgca ttacatcgac caaacgaggt tcgatcacgt ccgtgcaggc catctacgtg
      961 ccggccgatg acttgaccga cccggctccg gcgacagcgt tcgcacactt ggatgcaacc
     1021 accgtgttgt ctcggtcgct cgccgagttg ggcatctacc cggcggttga tcctctggac
     1081 tcgacttcac gtattctcga tccacagatc atcggtgagg agcattacaa gattgcgcgc
     1141 ggcgtgcagt ccgtccttca gcggtacaag gacctccaag acattatcgc gattcttggt
     1201 atggacgagt tgtcggaaga tgacaaaatg gtcgtggctc gcgcccggaa gatccagcgc
     1261 ttcctctcgc agcccttcca cgtcgccgaa gcatttaccg gcgcccccgg caagtacgtc
     1321 aaactcaaag atacggtccg cagcttcaga gagatcctcg acggcaagta cgatcacctg
     1381 ccggagcagg cgttctatat ggtcggcccg atcgaagaag cggtggcgaa agccgagaaa
     1441 atgggagtga aggtgtagcg gagcgagcag gatgtcctct gtgctcgcgc accccccacg
     1501 atgaagctgt ggtcggtgca tgcgcgcaat tgacgacatc ctgctcactc cgcttgataa
     1561 agtgtagagg gagagaaaag aaggaaaatg gcggggaaga ttctgttaga agtcgtgacg
     1621 ccggagaggc agctgctgag tcagcaggtg gatgaagtca tcgcccctgg gtctgaggga
     1681 gagttcggcg tgctgcctgg gcactgccat ttcctctcaa ctcttcgcat cggcgaactt
     1741 cgttatcgtg tcgatggtca gacccattcc atggccgtac tgtggagctt tgccgaagtc
     1801 acaccgacca aagtcaccgt catggcggaa gttgccgaaa aagctgaaga cattgatgtg
     1861 gaacgggcga ctgccaaggt cactgaggcg gagcgacgcc ttcaagccgg cggcttgccg
     1921 tccgaagtca aagaagccca gatcagtctc gaaaaggctc gtcttcgcaa gaaaattgcc
     1981 gaacgttctc aaaaaaccgg ccacgcctaa accatcaatt ctcttactgc atgacttaca
     2041 tcctaagtta tgcaatctcc caacgcacag tctattccgg catcctattg cgcctgaccg
     2101 acagagctga gatcactccg ccggtcaaga atgctggtta ctgtgttgtg cgatttctca
     2161 agggcccata ggcttcagtg gccgctgccg ctcgatgctg ctggacggct actgtcccaa
     2221 ctgaatactg atcacgatca cacctatgac tggatggcgg tctgatcctc tatccaggga
     2281 aaccgggtcc caactgaata ctgatcacga tcgcacccat gacatcatta accttgaagt
     2341 ctcgcttcgg gctgttggga tgggcattat gacagccgat acaggcctga gtgacggcca
     2401 gatcgggata caccgcttca taataacggg tgtcgcctac cttcgtgacc ccggtgaagg
     2461 gatggttggg ttgagtcaca atagtaccga gcgcaatact ttggagggga gttgtcgcga
     2521 catttcgctt gttgatcggc cacaagctga tcagccgata ctgtatgcct atgcccttct
     2581 tatcgaggtg ccgaccggat tccaatagaa attgggctgg caggggcaag gtgtgcatgt
     2641 cttcccaatt ttccgaggcg acgacgatcc ctttcgtttg catgcgttct acgacatgtc
     2701 tggtatacga atctcggtca gcctcgatga cggcatggat gtagtcggct accttgtcca
     2761 tcgacagaca gatcgatgtg gtactaggca aggtgcgagc cgggtacagg acaatcgcgc
     2821 ctcctattgt caatcccaac agagcagctc cccacagcat tttttgggag aacgtcatag
     2881 atacctcctt gttgtgcgcg cggattgatc ggctgtccgg tcacatcccg cgagcaggtg
     2941 aatggcgctg gtgtcaaaaa tgagaaatcg ttgtgtcagt ggacggacca atccccgtgt
     3001 cggatcagtc cgagaaataa tatgagcgga tcggaagggt ctgatgaaac tgtacggatc
     3061 aacaggtaca ccggtgttac cggcgggttc cctgaggggc aaacaatcgt gggaaggtac
     3121 caaagacatg gccccgttcc cttactcgca tgacctgacg cgcccacaca accatacgac
     3181 acagcagcat actctctcat ctatgccttg ttcaatcgca gcttccgcat ctcatggagc
     3241 acgggacaac aaatgatatt gcttcatgtc cattgttcga gtacaagtac atcgcgtctg
     3301 gtaaccaagt gcttccattg tctcatctcc cgacttgacc gctgatcatc catgcagttt
     3361 cctcttgagg ccaagctgaa ggacgagacg ccgattgagc tgctgccggc aggaaaccgg
     3421 gaccggctcg cgctggcaaa attatatgac cggatcgtgg cagagggcac gtcgtatcca
     3481 catgagggtc cggttacaga ggacgaattt ctggattatt gggtccgggg caagaggacg
     3541 gtggtggcgt atgaacggcc ccgaaagacg gatgcgaagt tgctcggcgc gttctatctc
     3601 aaaccgaact ggccaggccg cgcacgacat gtggcgaatg cgggattcat cgtcatgccg
     3661 gaatggcgga atcgcgggtt gggccggctc cttggcgccg tgatgctgga ttatgccgag
     3721 gagctgggtt atcgcagtgt gatcttcaac ctggtctttt ccgagaatca gggcgcgcgc
     3781 catctgtggg agaaacttgg cttccgtgtg ctcggcgccg ttcccggcgc ggttcgcacg
     3841 aacgacggta aataccaaga cgccctcatt atgtttcgct cgttgatcta agtcgagtgt
     3901 cccgtgactg cccaacaaca atggaatccc caacagtacg cagagcatgc gaggtttgtg
     3961 acggacctcg gcgcgccgct gatcgacatg ctggctctca aaccgggcga acgtgtcttg
     4021 gatgtgggtt gcggcgacgg agtgttgacc aagcagctca tggaccgagg ctgcgacgtg
     4081 atcggagtgg atgcgagtcc ggagatggtc gaggcggcca agctttgtgg cgtcaacgca
     4141 cagatcatgg atgggcaccg gcttcccttc tacgaagaat tcaacgcggt cttcagcaac
     4201 gcggcgttgc attggatggt gagaccagat gaagtcctgc acagcgtccg gcgggcgtta
     4261 cgaccaggcg ggcgtttcgt tgcagagttc ggcgggagag gcaacctcac caaactattg
     4321 gccgggcttc acaagattct ccgggcctac ggctgcgatc ccgctgccgt tcaaccctgg
     4381 tacttcccga ccgcagagga gtatcgtgcg aagctggaac agcaaggctt tcagctcgtc
     4441 tccatcgacg tatttcctcg tcctaccgag ttacccggcg atatcacaaa gtggttggag
     4501 atgttcgccc ggccgtttct tcttctcatc cccagcgcga gtcaggcgga attcctcacg
     4561 cgagcgcgtg aagcgctgcg accgatcttg gcgactcccg atggccgttg gacggttgat
     4621 tatgtccgcc tgcgctgcca cgccgtcaag ccgaccgcgc ctaggttctc tctctctcct
     4681 gagatttgac cgatgagacg cgggagcggt agggtatgcc gtgaccccgc ttcatctgct
     4741 cgtgacgctc gttctttccg ccggcgcgtg gccccttgat gagccgtggc tccttcagcc
     4801 ggtatcttcg gccaggacat tgaagggtga cgagttgctt cggatcggcg aaatccacga
     4861 cgtgcagaac cacttctcgg aagccctcac gtactatgat caagcgcgcg aatcgttccg
     4921 cgcgaccaaa cagcggcaag gcgaagcgac agccctgaca aaaatcggtt cggttttcga
     4981 acgacagggc cggagagaag ccgccgccgc gcagcttcgt gatgccttgg cgctcttctc
     5041 aaaagttccc gccagcccgg ctcatggaga tgcgctgttg acgctcggca aagtctctgc
     5101 ctggttggga tcgcgagaag aagccggccg tttgtttgta cgagcggcgg accgatacag
     5161 ccggtcgcgc aacattcaag gagcggtttc ggcaagaata caactcgggc tcctacggat
     5221 cggcgatgga ttgactcaag aaggactcca actcctcgaa caggtgctcc aggacgccca
     5281 caaccgtcaa gacaacgatc agacactcgc ggcattgttt gcagtcggtg atgcccgttg
     5341 gatcatggat gagccggacg cggcggagca gtattacgca cgggcgcttg cgctggtcga
     5401 gcaaagacct caagccggca tggaagcggc gttgcgttac cgcatgtccg ctgtctatga
     5461 cgccatcggt caacaggaga aaggaatagg atcggcgaag cgcgcggtga ctctctatca
     5521 atcgttacgc gatccgtccg gtgaagcggc gtcttgggcg ctgctggctt ccctctatga
     5581 agcgctcgga aagcatcaag aggcggacga ggccggacag cgcgcgcttg cgattttccg
     5641 ccggcgacag gttatcgtgc atgccgccca cccatcggcg aattccctcc tctcggagtc
     5701 gagatgaaac gatagagcca ttgccgttcc gaagcagacc tacgaaagaa gcaaaccaac
     5761 gatatctcca attgcatttg gtcgatgctc tttagtagcg tacccgccca tgattaccga
     5821 gttcgtcgtg acgtcgggag aacagcccaa acggctggat ctcttccttg tcaatcgaga
     5881 gcgagacatc tcgaggtcgg cgttgcagcg gttgatcgag ctgggtcgca ttcgaataaa
     5941 cgatcaggtc gtgaaggcca gtcacaagat caaacccggt gataggatca ccatggacgt
     6001 gccgaagccg gagccgctct cgatcaaagg agaggcgatt ccgctggaca tcctcgttga
     6061 agatgacgct cttctcgtct tgaataagcc gtcagggatc gtcgtccatc ctgccccagg
     6121 caactggacc ggtacgcttg tcaatgcatt actgcatcac tttcaaacgt caggaggaac
     6181 catttcaacc attgggggga aagagcggcc cggcctcgtc catcgactgg acaaagaaac
     6241 atccggtatc atggtgatta caaaaacgga tgcggcacat cgtgtccttg cagcacagtt
     6301 caagctccat accatcatcc gggtctatga ggcactggtc tggggcgtgc ccaagaaagg
     6361 cagaggattg atcgaactgg ctattggtcg ggacgtcaaa gaccggaaga aattttcctc
     6421 gagaacg
//
LOCUS       DATMTI010000349         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_564791, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000349 DATMTI010000000
VERSION     DATMTI010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564791"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2145)
                     /gene="mrdA"
                     /locus_tag="VN039_05815"
     CDS             complement(<1..2145)
                     /gene="mrdA"
                     /locus_tag="VN039_05815"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWV45532.1"
                     /translation="MSRRRFSSRLRIQFLGVFMLLGVGSLGVRLWWIQVAHGQEWTAQ
                     IRGSSQATVRIPSVRGEIKDRNGVTLVQNRASYEVDFYLPEMVKGYRERFGSPPTSEY
                     RAIISGMPKDMKEPDIIRIVNNGIVPRLNELDLARDYNANKLQRHFRNDTEVPFQYIK
                     DIDFPTMAKFAEHDVGLPGVDLAIKPVRSYVYGALGSHILGYVGAPDDIDKEEAKKFT
                     FYQADVEGKSNIEKSMDEYLRGKPGVRYLRRNAKGAIDGVLREDPPQQGANVFLTIDA
                     RIQAITEEALRSVGRGAAVVVDPNNGEILAMASVPSFDPNTFIPSIKAKDWAVLQKDE
                     ANPLINRAVSGFPPGSTFKLITSLAGLRRGLAKQRFTCSGGVQYGDHYFQCWIAEKHG
                     SHGSLSLPDAIKVSCNAFFYQYGNAAGIESIDRVGELLGLGKPSGLNLTGEQPGVLPG
                     PDWMRINYPQERWSQAYTANVSIGQGYDLATPLQMAMAYAALANRGTSYYPRLVKKVL
                     NQDGTPVLDEDGKIAVPDQPKVHADFRSEISPADIELERRGFWNVVNADGGTGGRARI
                     NGVQMAGKTGTAQAKYKGREDTISWFVCWAPFDNPKYTVAVMVQGGEHGGTVACPIAA
                     RIMERSLAMDEGKLDAQVAWLPPAHKPNPFQMIKDVSFKDSGINGGGNDDENANGSQG
                     SDVQMASAGGDPDVEPEADSQGKVARRASAPVA"
     gene            complement(2142..>2557)
                     /locus_tag="VN039_05820"
     CDS             complement(2142..>2557)
                     /locus_tag="VN039_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45533.1"
                     /translation="MALPFPLMLALALFAGVLLDALTVQVIGGKVEISAGSSILIYGA
                     LAGIMHGLRPLFARGRWEVHCILSGVFTSLIVLAQYLMITFRRGSLVFTREIWWQIGG
                     PGLIAMAIAPIFFWGLQWLAQITAYSYGPQRGQIE"
BASE COUNT          570 a          768 c          649 g          570 t
ORIGIN      
        1 agctaccgga gctgaagctc gtcgggctac tttcccctga gaatctgctt cgggctcgac
       61 gtcgggatcc ccgcccgcgc tcgccatctg tacgtctgag ccttgcgaac cgttggcatt
      121 ctcgtcgtca ttaccgccac cattgatacc cgaatccttg aaactcacat ccttgatcat
      181 ttgaaacgga ttgggcttat gcgcaggagg aagccatgcg acctgggcgt ccaattttcc
      241 ctcgtccatc gccaatgaac gttccatgat ccgcgccgcg attgggcacg cgaccgtgcc
      301 gccgtgttcc ccgccctgca ccatcactgc aacggtgtat ttgggattgt cgaaaggcgc
      361 ccagcacaca aaccacgaaa ttgtgtcttc tctgcctttg tatttcgcct gagcggtacc
      421 cgtttttcct gccatctgga caccgtttat gcgggctcgt ccgccggttc caccgtctgc
      481 attcaccacg ttccagaatc cacgtcgttc caattcgatg tcggcaggtg aaatctctga
      541 gcggaaatcg gcatgcactt tcggctggtc aggaacggcg atctttccgt cctcgtccaa
      601 taccggtgtg ccatcctgat tgagcacctt tttcacgaga cgcgggtaat aagaagttcc
      661 gcgattagcg agggctgcat aggccatcgc catctgcaga ggcgtggcca gatcgtatcc
      721 ctggccgatg gaaacgttcg ccgtgtacgc ttgcgaccat ctttcctggg gatagttaat
      781 cctcatccag tctggcccag gcaacacacc tggctgttcg ccagtcaggt tcagtccaga
      841 aggttttcct aaaccaagaa gctcaccgac acgatcgatc gactcgatgc ctgccgcgtt
      901 accgtactga taaaagaaag cattacacga caccttgatg gcgtccggca aggagagtga
      961 accatgcgag ccatgtttct ccgctatcca gcattgaaaa tagtgatcgc cgtactgaac
     1021 acctccgctg caggtgaacc tctgcttggc caaaccacgg cgcagtccag cgagcgaagt
     1081 gattagcttg aacgttgaac caggtgggaa cccgctcacc gcacgattga tcagcgggtt
     1141 ggcttcatcc ttctgaagca cggcccaatc cttggccttg atgctcggga taaatgtatt
     1201 tggatcaaac gagggcaccg atgccatggc gagtatttcg ccgttatttg gatcgacaac
     1261 caccgcagcg ccacggccaa cggatcgcag tgcttcctct gtaatcgcct ggatacgcgc
     1321 gtcgatcgtc aggaaaacgt tcgcgccctg ttgcggtgga tcttcacgca aaaccccgtc
     1381 aatcgcacct ttagcattac gtcgcagata ccgcacgccg ggtttacccc gcaggtattc
     1441 gtccatcgac ttttcaatgt tcgattttcc ttcgacgtcg gcctgataga acgtgaactt
     1501 cttcgcttcc tccttatcga tgtcatccgg cgcaccgacg taaccaagaa tatgcgatcc
     1561 gagcgcgccg tacacgtacg agcggaccgg cttaatcgcc aaatcaacac cgggcagccc
     1621 gacgtcgtgc tcggcgaact tcgccatggt aggaaaatcg atatccttga tgtactggaa
     1681 aggaacctca gtatcgttgc gaaaatgtcg ctgcaacttg ttggcattgt aatcgcgcgc
     1741 gagatccagc tcgttcaaac gggggacaat cccattgttc acgatcctga tgatatcggg
     1801 ctctttcatg tctttcggca ttccgctgat gatcgcccga tattcactgg tcggcggtga
     1861 accgaaccgt tcgcggtagc ccttgaccat ttccggcaga taaaaatcca cctcgtagct
     1921 ggcgcggttt tggacaagtg tgacaccgtt tcggtctttg atttcgccgc gcaccgacgg
     1981 aatccgcacg gttgcctgcg agcttccgcg aatctgcgcc gtccattcct gaccgtgcgc
     2041 cacttgtatc caccagagtc ggactccaag tgagccgacg cccaacaaca tgaacacccc
     2101 gagaaattgt atccgcagac gggaactaaa gcgtcgccta ctcattcgat ttgtcccctt
     2161 tgcggaccgt atgaatacgc ggtgatctga gcaagccact gcaatcccca aaagaaaatg
     2221 ggagcgatcg ccatcgcgat aagtccgggt ccgccaatct gccaccagat ttcacgcgta
     2281 aaaacaagag agccgcgccg aaacgtaatc atgaggtatt gggccagaac tattaacgag
     2341 gtaaaaacgc cactcaggat acaatgcact tcccagcggc cgcgggcaaa gagcggtcgc
     2401 aagccgtgca ttattcccgc cagcgcaccg tagatgagta tggatgatcc agccgagatt
     2461 tcaaccttac ctccgatcac ctggacggtt agcgcatcca gtagcacccc ggcgaacaac
     2521 gcgagagcga gcatcagggg aaagggcaat gccatcg
//
LOCUS       DATMTI010000350         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_691906, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000350 DATMTI010000000
VERSION     DATMTI010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691906"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="ispE"
                     /locus_tag="VN039_05825"
     CDS             complement(<1..624)
                     /gene="ispE"
                     /locus_tag="VN039_05825"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWV45534.1"
                     /translation="MTNPASRSTSSPPFITVFAPAKINLVLRILDRRFDGYHNLWSLM
                     QTVQLEDELALSLSDNHSTITLRCDEPSLKTDPSNLVYRAAAAVLESSGRTVGLDMIL
                     TKRIPMGAGLGGGSSDAAATIVGLNRLLNLGWSRERMVHIGQTLGSDVPFFFFAPSAI
                     VEGRGETVAPVQIKGSRWVVLVNPGFPVETKWAYQQLSVSRTGVQPFS"
     gene            complement(617..2002)
                     /locus_tag="VN039_05830"
     CDS             complement(617..2002)
                     /locus_tag="VN039_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV45535.1"
                     /translation="MEKILIVDDEQSLRDVLSIMLKRAGYVVTSAMDGEEAIELLNKE
                     IFDLVITDLRMPKIDGMEVLRAVKSASPETVVLIITAFATADSAVDAMKQGAYDYLTK
                     PFQVDEVQLIIRNALEKRRLTTENMLLKREMASQSSFAQLVGQSEAMQKVFDVVRKVA
                     DSKSNVLICGESGTGKELVARAIHYNSARSALPFVAVNCSAVPETLLESELFGHMKGS
                     FTGAISNKAGLFEIADGGTIFLDEIGDTTPTIQVKLLRVIQEREFRRVGGNQDIKVDV
                     RVVAATNKELERAVAEGSFREDLYYRLDVIPIRLPPLRMRTGDIPLLVNHFLERFSKE
                     SGKPKPVISPEAMHVLLGHEWRGNVRELENLIERVVAFSTEGPVTDAEVRGWLHRPAT
                     QSQQPAMPLDLTDEGLDLEGLINGIEKDLLLKALERSKWVKKKAARMLRLNTRSFRYR
                     LEKYAIKGGRD"
BASE COUNT          473 a          654 c          525 g          501 t
ORIGIN      
        1 cgaaaacggc tgtactcctg tgcggcttac agaaagctgc tggtacgccc actttgtttc
       61 gaccgggaac cccggattaa ccagcacaac ccaccgactc cccttgattt gcacaggtgc
      121 taccgtttcg ccacgtcctt ccacgatcgc agaaggagcg aaaaaaaaga acgggacatc
      181 gcttccgaga gtttgaccga tgtggaccat cctctctctc gaccatccca aattcaatag
      241 ccggttcaga ccgacgatcg tcgctgccgc atcgctgctt ccgcctccca acccggctcc
      301 cattggaatt cgctttgtga ggatcatgtc caatccaaca gttcggccgc tgctttcgag
      361 taccgccgct gcagcgcgat acaccaggtt ggaagggtcc gtcttcaagg aaggttcatc
      421 gcatcgcaag gtgatcgtcg aatggttgtc gctaagggaa agtgcgagct cgtcctccaa
      481 ctgcacggtc tgcatcagag accagaggtt gtgataaccg tcaaaccgac ggtcgagaat
      541 ccgaaggaca aggttgatct tggcgggtgc gaaaacggta atgaaaggag gtgagcttgt
      601 cgatcgggag gcaggattag tcacgacctc cttttatagc atacttctcc aaacgatacc
      661 gaaacgatct ggtattcaga cggagcatcc gtgcggcttt ctttttgacc cacttcgatc
      721 gttcaagcgc cttcagcaat agatcctttt cgattccgtt gatgagccct tcgaggtcca
      781 agccctcgtc agtcagatcc agcggcatcg ccggttgttg agactgtgtc gcaggtcgat
      841 gaagccatcc tcgcacttcg gcgtcggtca ccggcccttc cgtggagaat gcgacgaccc
      901 gttcgatcag attctccaac tcacggacat tgccgcgcca ttcgtgcccc agcaaaacat
      961 gcatcgcctc cggactgatc acgggtttgg gcttaccgct ctccttcgaa aaccgttcca
     1021 gaaagtgatt gaccagcaat ggaatatcac ccgtgcgcat acgtaacggc ggaagccgaa
     1081 tggggatcac atccaaacga tagtagagat cttctcgaaa tgaaccttcc gcgactgctc
     1141 tctcaagttc cttattggtg gcggccacca cacgtacgtc gactttgata tcttgattgc
     1201 cgcccactcg ccgaaactcc cgttcttgga tgacacgcag caacttcact tgaatcgtcg
     1261 gggtcgtatc accgatctca tcgagaaaaa tggtccctcc gtcggcgatc tcgaacaacc
     1321 ccgctttgtt tgagatcgct ccggtgaacg atcccttcat gtggccaaat agctcgctct
     1381 ccaacaaagt ttccggcacc gcgctgcaat tcacggccac aaatgggagg gcacttctgg
     1441 cgctgttgta atgaatcgcg cgcgcgacca actcttttcc cgttccgctt tcgccgcaga
     1501 taaggacatt gcttttcgag tccgcgactt tccgtacaac gtcgaacacc ttctgcatcg
     1561 cctcgctttg gccgaccaac tgcgcgaatg atgactggct cgccatctcc cgcttgagaa
     1621 gcatgttctc ggtcgtgagg cgccgctttt ccagcgcatt ccgaatgatc aactggactt
     1681 catcgacttg gaacggcttc gtcagatagt cgtacgcgcc ctgtttcatg gcatcaacgg
     1741 cggaatccgc ggtggcaaag gccgtgatga tcagcacgac tgtttccgga gaggcggact
     1801 tgacggccct aaggacctcc attccatcga tcttgggcat ccgcaaatcg gtgatgacca
     1861 gatcaaagat ttccttgttc agaagctcga tggcttcttc gccgtccatc gcactcgtca
     1921 caacatatcc cgcccgtttg agcatgatgc tcaatacgtc gcgcaaactt tgttcgtcat
     1981 caacgattag gatcttttcc acggttctct accttcgtgc cagagtcgca ctccagaatc
     2041 caccgtacga ggcaagcaga ctccaaatcg tgtcccttgc ccctcctggc tttccacctt
     2101 gatccaccct ccgtgaagat cgacgatgcg atggactgcg gccaaaccca atc
//
LOCUS       DATMTI010000351         1959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_13563, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000351 DATMTI010000000
VERSION     DATMTI010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13563"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..644)
                     /locus_tag="VN039_05835"
     CDS             complement(84..644)
                     /locus_tag="VN039_05835"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HWV45536.1"
                     /translation="MNTIKSFCLLFVIVMLSGCITEATLELTKAPFDATTALTNGTSK
                     AVGELLEPTQKFLSSTTPGTAAIERFTRAREKTELFVGYSSEHLRAESARGSGEHLAS
                     LAVLAGIPADRRLQFQEHMRETYSTMFDESLLPREVRLRIVNAAWSEGYGRLESETIK
                     HQETGISDIDLKIGKARHSTAFMSVR"
     gene            complement(1279..1354)
                     /locus_tag="VN039_05840"
     tRNA            complement(1279..1354)
                     /locus_tag="VN039_05840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1319..1321),aa:Arg,seq:acg)
     gene            complement(1546..1872)
                     /locus_tag="VN039_05845"
     CDS             complement(1546..1872)
                     /locus_tag="VN039_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWV45537.1"
                     /translation="MTNYHRWFLICLSLLITMACSSTSPTPTGETATLTWDANSEPDL
                     QGYKIYLATASGGYGAPIATLPIDVTSYTVTGLESGTTYFFSVTAYNSSGAESSFSNE
                     VSKTLL"
BASE COUNT          465 a          500 c          554 g          440 t
ORIGIN      
        1 cggaatgtca tgcgtgccat gaaggccacc ggcagacatc gcgacatcct ccatacgtca
       61 tgccactccc ctcacagacg ggctcatcgc acagacatga acgcagtcga gtgccgagcc
      121 ttgccgatct tcaagtcaat atcagagatc cccgtctcct ggtgtttaat tgtctcggat
      181 tcgagtctgc catatccttc cgaccatgcg gcattcacaa tgcgcaacct cacttcacga
      241 ggcaggaggg attcgtcaaa catcgtcgaa taggtctcgc gcatgtgctc ctgaaattga
      301 agacggcgat ccgcaggaat gccggcaagg acagcgagag aagccaggtg ttctcctgat
      361 cctcgtgcgc tttcggcccg caagtgctca gaggaatagc ccacaaagag ctccgttttc
      421 tccctggcac gcgtgaagcg ctcaatcgcg gctgttcccg gcgtcgtgct ggagaggaac
      481 ttttgcgtgg gctcaagcaa ttcgcctacg gctttcgatg tgccattggt caacgccgta
      541 gtcgcatcga agggggcctt cgtcagttcc agcgtcgctt ccgtgatgca gccagacaac
      601 attacaatta caaacagtaa gcaaaacgat ttgatagtgt tcataatata ccccctagtt
      661 atctttcacg tagctattct attgacgcag tgtaacaaac catattcttt gttgctccta
      721 ggagaaacga taggtgagga cagttcgatg agctgccaag aatgtcgttc catcgcatca
      781 acacggcgat agagacagac gagaagagca gtttaggttg aaatagggcc aggttgacct
      841 tgaaaacggc gttctcacgc ccaagggcgg gaagactcgc cacgtgccct tgagcgacga
      901 ggcaaaggct attctgcggt cgtttgactc gtttctccaa tcggcctggg tctttccggg
      961 gatgcgggat gaaactcaac cgatggatag ccgcgccttt ttgcgccgat catttgagcc
     1021 gggtctacgg aaggcaggta tcgctggggt ctgctggcat acactccgac acacggcagc
     1081 aagccgacga gtaatggccg gagtcgatct tgtcgcagtg aaggaaattc tcggacaccg
     1141 tgatattgag acgacgctga gatatgccca ccttgcacca ggacaccttc gggatgctgt
     1201 gaatcgaggg agtcttaggt ggaaccgtga ccactcagga gaggggagga gcggaagagg
     1261 agcaacccat tgattctatg gtgcgcccga caggacttga acctgtaacc cccagatccg
     1321 tagtctggtg ctctatccat tgagctacgg gcgcgtgccg aaccgatgct gaggtgtgag
     1381 ttctgagtta aagggctagc ttgaggacga tgggtcagga aggagtttcg tctcaggact
     1441 tagcactttc gtctcagcac ttcccggagt cagtggttat acagttctcg gagagagagg
     1501 gtcaagagac gaacactgga aaagtccgga gtcggcgaag gttagttaga ggagagtctt
     1561 gctgacttcg tttgagaagg aactttcggc gccactcgaa ttgtacgcgg tgaccgagaa
     1621 gaaataggtg gtaccgcttt ccagaccggt cacggtgtag ctggtaacgt ctatgggcaa
     1681 cgtggcaatc ggcgcaccat agcctcctga ggccgttgcc agataaatct tgtatccctg
     1741 aaggtccggc tcgctattgg catcccatgt aagagtggcc gtttcaccgg ttggcgtggg
     1801 gctggtcgaa gagcacgcca tggtaatcag cagggacaaa cagatcagaa accaacgatg
     1861 ataattggtc attgatctcg ataagtccat atcacactct taccgcgtga tggtccattc
     1921 ggaatctata cacgagcaaa gtttgttccg gtttcagcg
//
LOCUS       DATMTI010000352         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_777129, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000352 DATMTI010000000
VERSION     DATMTI010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777129"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VN039_05850"
     CDS             <1..212
                     /locus_tag="VN039_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWV45538.1"
                     /translation="RYANMKELRFHAADGVWRVAFAFDPRRHAILLVAGDKSGGSERR
                     FYEALIRKADKRFYDHLGRLKQKRK"
     gene            215..610
                     /locus_tag="VN039_05855"
     CDS             215..610
                     /locus_tag="VN039_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWV45539.1"
                     /translation="MATHVRDIMKGLSPSRRKKIETRASELIAEEMSLRDLRQALTLT
                     QQRMAKTLGIGQEGVSRLEQRSDLLLSTLRSYIEAMGGQLSLIAEFPNREPVALSGLC
                     AMETTPRRTPRPSRRMRMRPHAAQSKGVY"
     gene            652..1185
                     /locus_tag="VN039_05860"
     CDS             652..1185
                     /locus_tag="VN039_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWV45540.1"
                     /translation="MDCILVRHGIAVEPDEWEGTEENRPLTEKGKRRARQAAEGLAAL
                     DCKPTHLFTSPFVRAYDTARLLRAVVCPTLKVETREELVVGAKPEQVVALLHTLPSDS
                     AVVCVGHEPLLGEVVSLLLCGKALPNFPLKKAGAACIHADEVIRPGHGRLRWWLQPMQ
                     LRTLGKRAQNKKEVKRV"
     gene            complement(1182..1904)
                     /locus_tag="VN039_05865"
     CDS             complement(1182..1904)
                     /locus_tag="VN039_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45541.1"
                     /translation="MADARFFGDGLKYLDPSDLKGKLIAIEGTDGVGRSTHIEMLQEW
                     LEVQGYGVMTTGWTRSNLMSKTIEMAKAGNIVDRWSLSLLYATDFADRLEHQIIPALR
                     SGFVVLTDRYIYTAFARDFVRSADRTWIRDVFGFALIPDLVCYLRIDVETLALRVIET
                     TGMNYWESGMDLRLGGDLYDSFKKYQSLLIEEFDKMAIEFRFDVVDARKSPEEIQDEL
                     RGHILPVLQNNKPTIDTETASS"
     gene            complement(1852..>2296)
                     /locus_tag="VN039_05870"
     CDS             complement(1852..>2296)
                     /locus_tag="VN039_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45542.1"
                     /translation="LADRYMYTAFARDVVRGVANEWVRKLYAFSIKPDMAFYFKVPIE
                     VAISRLSRGTRGHFKYYEAGMDMDLSPDITESFRLFQSRILAEYDKIVDECGLLTMDA
                     TQDIETQQEHMRALVEEALHGYKPKRGTYGRRTLFWRRFEVSRSE"
BASE COUNT          513 a          623 c          646 g          514 t
ORIGIN      
        1 cgcgctacgc gaacatgaag gaacttcgct ttcatgctgc tgatggagtc tggcgtgtcg
       61 ctttcgcgtt tgatccccga cggcatgcca tcctgcttgt cgccggggat aaatcagggg
      121 gaagcgaaag acgtttctac gaagcactca tccggaaggc cgacaaacgc ttctatgacc
      181 atctcgggcg attgaaacag aaaaggaaat agccgtggca acccatgtac gagacatcat
      241 gaagggcttg agccccagcc gccgcaaaaa gattgagacg cgcgccagcg agctgatcgc
      301 agaggaaatg tccctaaggg atttacggca ggccttaact ctcacgcagc aacgtatggc
      361 taagaccctt ggcattggcc aggaaggtgt atcgcgtctc gaacaacgca gcgatttgct
      421 gttgtccact ctgcgaagct acattgaagc catgggtggt caactctcac tgattgccga
      481 atttcctaac cgcgagcccg tcgccctctc agggctatgt gctatggaaa cgactcctcg
      541 ccgtaccccg cgcccaagcc gcagaatgcg catgcgaccc catgccgctc aatctaaggg
      601 cgtctactga ctccctgttc tgcgtgagtc tcactatgtg gagaggtgat tatggactgt
      661 attctggttc gtcacgggat cgctgtagag cctgatgagt gggaagggac ggaagagaat
      721 cgtcctctca cggagaaagg gaaaaggcgt gcgcggcagg ctgccgaagg gttggccgca
      781 cttgattgta agccgaccca tctattcacg agtccgttcg tgcgggccta tgatacggca
      841 agactactgc gtgcggtcgt ctgtccgact ttgaaggtcg aaacacgcga ggagctggtg
      901 gtcggagcga aacccgaaca agtggtagcg cttcttcata cgctcccgtc agactcagcg
      961 gttgtgtgtg tgggacatga gccgctgttg ggtgaagtcg tgagtttatt gctttgcgga
     1021 aaagcccttc ccaattttcc gcttaagaag gccggagccg cgtgtattca tgcggacgag
     1081 gttatcaggc ccggtcacgg gcggctgcgc tggtggcttc agccgatgca gctccggact
     1141 ttagggaagc gcgctcagaa caagaaagag gtcaaacggg tttaagaaga ggctgtctct
     1201 gtgtcgatcg taggcttgtt gttctgtagt acagggagga tatgtcctcg gagctcgtct
     1261 tggatttctt cgggcgactt tctcgcatcg acgacatcga agcgaaattc aatcgccatc
     1321 ttatcgaatt cttcgatcaa cagcgattgg tattttttga aactatcata gagatcgcct
     1381 ccaagacgga gatccatgcc ggattcccag taattcattc cggtggtctc gatgactcga
     1441 agcgccaggg tttccacatc gatccgtaag tagcagacca agtcggggat caacgcaaac
     1501 ccaaacacgt cacggatcca tgttcggtcg gcgctccgca caaagtcgcg cgcgaacgcc
     1561 gtgtagatgt aacgatccgt cagcacgacg aatccggatc ggagagccgg aatgatctga
     1621 tgctcgaggc gatcggcgaa atccgtcgca tagaggagac tgagcgacca acgatcgacg
     1681 atattgccgg ccttcgccat ttcgatggtt ttggacatga gattggaacg ggtccacccg
     1741 gtcgtcatca cgccgtaccc ctggacctcc agccactctt gcagcatctc gatgtgggtc
     1801 gatcggccga ctccgtcggt tccctcaatg gcgatcaact tgcctttcag gtcactcgga
     1861 tctagatact tcaaaccgtc gccaaaaaag cgtgcgtcgg ccataggtgc ctcgttttgg
     1921 tttatagcca tggagcgctt cttccaccag cgcccgcatg tgttcctgtt gcgtctcgat
     1981 gtcttgggtc gcatccatcg tcaagagacc gcattcatcg acgatcttgt cgtattccgc
     2041 gagaatccgt gattgaaaga gccggaaact ttcggtgatg tccggactca gatccatgtc
     2101 catgcccgct tcatagtatt tgaaatggcc gcgagtccct cgcgagagcc gggagatggc
     2161 gacttcgatc gggactttga aataaaacgc catatcgggc ttgatcgaaa acgcgtagag
     2221 tttccgcacc cactcgttcg ccacgcctcg caccacatcg cgcgcaaacg ccgtatacat
     2281 atatcgatcg gcgagg
//
LOCUS       DATMTI010000353         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_395777, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000353 DATMTI010000000
VERSION     DATMTI010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395777"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VN039_05875"
     rRNA            <1..993
                     /locus_tag="VN039_05875"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1123..1199
                     /locus_tag="VN039_05880"
     tRNA            1123..1199
                     /locus_tag="VN039_05880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1157..1159,aa:Ile,seq:gat)
     gene            1218..1293
                     /locus_tag="VN039_05885"
     tRNA            1218..1293
                     /locus_tag="VN039_05885"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1251..1253,aa:Ala,seq:tgc)
     gene            1379..1600
                     /locus_tag="VN039_05890"
     CDS             1379..1600
                     /locus_tag="VN039_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45543.1"
                     /translation="MLIELQSAALKAVRSLTIREGKGAFGLDESPDAQRVELYLARAR
                     EAQKARAWVGANTPITVALLKLTSRDELF"
     gene            1648..>2473
                     /locus_tag="VN039_05895"
     rRNA            1648..>2473
                     /locus_tag="VN039_05895"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          596 a          623 c          809 g          445 t
ORIGIN      
        1 agcgttaatc ggaattactg ggcgtaaagc gtgcgcaggc ggttcgttgt gtcggcggtg
       61 aaagccccgg gctcaacctg ggaatggccg ttgaaactgg cgaactggag tgtggcagag
      121 gggggtggaa ttccgcgtgt agcagtgaaa tgcgtagaga tgcggaggaa caccgatggc
      181 gaaggcagcc ccctgggctg acactgacgc tcaggcacga aagcgtgggg agcaaacagg
      241 attagatacc ctggtagtcc acgccctaaa cgatgtcgac tgggtgttgg ggaagcgatt
      301 tcccagtacc gtagctaacg cgtgaagtcg accgcctggg gagtacggcc gcaaggttaa
      361 aactcaaagg aattgacggg gacccgcaca agcggtggat gatgtggatt aattcgatgc
      421 aacgcgaaca accttaccta cccttgacat gtccggaagc ccggtgagag ctgggggtgc
      481 ccgaaaggga accggaacac aggtgctgca tggctgtcgt cagctcgtgt cgtgagatgt
      541 tgggttaagt cccgcaacga gcgcaaccct tgtccctagt tgctacgcaa gggcactcta
      601 gggagactgc cggtgacaaa ccggaggaag gtggggatga cgtcaagtcc tcatggccct
      661 tatgggtagg gcttcacacg tcatacaatg gccggtacag agggttgcca aagcgcgagc
      721 tggagccaat ccccgtaaaa gccggtcgta gtccggattg cagtctgcaa ctcgactgca
      781 tgaagtcgga atcgctagta atcgcggatc agcatgtcgc ggtgaatacg ttcccgggtc
      841 ttgtacacac cgcccgtcac accatgggag tgggttctac cagaagtagg taggctaacc
      901 gcaaggaggc cgcttaccac ggtaggattc atgactgggg tgaagtcgta acaaggtagc
      961 cgtatcggaa ggtgcggctg gatcacctcc tttctcggaa gcagggaaca gaggtcaggc
     1021 aacagggaac agcgatggac ccgggagcct gcactgtcct ccctcacacg ctttcagtac
     1081 ccacaaccta tcgattgcga cacgaccgca cggctttggc acgggcctgt agctcagctg
     1141 gttagagcac cgtcttgata aggcgggggt cgatggttcg aatccatcca gacccaccag
     1201 gaacacggca ggacgagggg gctgtagctc agctgggaga gcacctgctt tgcaagcagg
     1261 gggtcgtcgg ttcgatcccg atcagctcca ccaggtcagg caacggaggt caggaaacag
     1321 gtaacagacg aacgacctcg gcaacagcta tcgattgcgg caaggacagt gttcagccgt
     1381 gctgattgag cttcagtcgg cagcgctgaa agctgtgcgc tctttaacaa ttcgggaagg
     1441 taagggtgca ttcggacttg atgagagtcc ggatgcgcaa agggttgagt tgtatcttgc
     1501 ccgtgcgcgc gaagcacaaa aggcgcgtgc atgggttggc gcaaacacac cgataacggt
     1561 ggctttgctg aagctcacgt cgcgggacga gttgttctga cttctgtcct ctgacttctg
     1621 acttctgacg aggcgctgcc gttatggggt caagcgacta agtgcatgtg atggatgcct
     1681 tggcgattac aggcgatgaa ggacgtggca acctgcgaaa agctccgggg agctggtaaa
     1741 cgagcatcga tccggagatg tccgaatggg gaaacccggc ccaccgttaa gcggctttcg
     1801 gcgcgcgcca gcgcgctcaa agccactcgc ttaagcgagc gaggaggctg cttaacggtg
     1861 ggtcactcct gactgaatcc ataggtcggg tagagcaaac ccggtgaact gaaacatctc
     1921 agtagccgga ggaacagaaa tcaaccgaga ttccgctagt agtggcgagc gaacgcggag
     1981 gagccggcaa gttttagcga ttcgtctagc agaacgctct ggaaagggcg gccgcagtgg
     2041 gtgacagccc cgtatgcgaa agatggatcg tggaaccaag cttgcgacaa gtagggcggg
     2101 acacgtgaaa tcctgtctga agatgggggg accatcctcc aaggctaaat actcgtaatc
     2161 gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag aaccccggaa ggggagtgaa
     2221 acagatcctg aaatcgcatg catacaaaca gtgggagccc cagctcaatc ggccttcttt
     2281 gacgcgcgcg cggcagcatc gcgcgggcgg caaaagcccc gccgtcgaaa agagatcctg
     2341 agacgggatg ctcgcggcgg ggattttgaa cgcgcgagcg attcgacgcg cgggcggcaa
     2401 aagacctcca ggatgaaaca gatccatgcg caagacgcac gtggatcgga gggccgattg
     2461 agctggggtg act
//
LOCUS       DATMTI010000354         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_565017, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000354 DATMTI010000000
VERSION     DATMTI010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565017"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2708)
                     /locus_tag="VN039_05900"
     CDS             complement(<1..2708)
                     /locus_tag="VN039_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HWV45544.1"
                     /translation="MTIPGFPRRQGLYDPQHEKDSCGIGFVVNIKGKKSHDIVRKGLQ
                     VLENLTHRGAQGSDPYTGDGAGILLQVPHTFLKRVAGDAGLSLPEVGEYGVGQLFLPP
                     NADSRRLCEKLFTEILVEEGLRLLGWRDVPVKSDQIGAQARTTEPFMRQIFIARDALN
                     EARFERKLYVIRKRVEKAVAESAIQGREHFYVSSLSAYTIVYKGLLLPHQMSAYYQDL
                     TDERMVSALALVHSRFSTNTFPTWPRAHPYRYVCHNGEINTLKGNVNWMKARQGRLHS
                     ELFGKDMEKLFPIVSENQSDSACLDNTLEFLLLGGRSLPHAMMMLIPEPWVANAQMDL
                     DRRGFYQYHAAMMEPWDGPAAVCFTDGKMIGATLDRNGLRPCRYQVTTDGLVVLASEA
                     GVLPADAKDIRMKGRLQPGRMFLVDTVQGRIIDDEEIKADIVGRKPYRSWVTQYGVSL
                     DELPDPLNVPQPDHPTIRQRQQAFGYTVEELKMVITPMIVTGEEAISSMGTDTPLAVL
                     SDRPQLLFKYFKQLFAQVTNPPIDPIREQLVMSLVTNIGPKPNLMDESPESCRRIKVQ
                     QPILTNADLQKIRGISDPHFKSKTLRMLFRVAEGPDGLGEAVDELCRQASQAIKEGYT
                     FLILSDRGVDEQWAPIPSLLGISAVHHHLVRECTRTEAGLILETGEPRDVHQFACLIG
                     YGAGTINPYLVFESLVDMERDNYLPEGLDAQTAEGKFIKAINKGLLKIFSKMGISTVQ
                     SYCGAQIFEAIGLNRELIDRYFTGTPSRVEGVGIRDIGEETLRRHSVAYEPSAIRQLD
                     FGGEIHYRIQGEHHNWNPETIYKLQHASRSNDPKTYAEFSQIVNDESKQRSNLRGLLD
                     FKFLPRPIPIEEVESAKEIVKRFNTGAMSFGSISKEAHETLAIAMNR"
     gene            2928..>3573
                     /locus_tag="VN039_05905"
     CDS             2928..>3573
                     /locus_tag="VN039_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWV45545.1"
                     /translation="MAQGPAIATLHSMSDVWDAYRGELDGVEDRVKKNLDSSVTLVNT
                     VAAHILSGGGKRIRPLLLLLSARLCGYPGTEHHQLGSIVEFIHTATLLHDDVVDDADL
                     RRGRRTARKVWGNQISILVGDYLYTKAMCKVVEFQSQGINEVLAEACKKMAEGEVLQL
                     YYNGNPSMPEIDYIKIVEHKTAGLIAAACRMGAILAEASETQQMALFRFGQYLGI"
BASE COUNT          744 a         1039 c         1002 g          788 t
ORIGIN      
        1 cggttcatcg cgatcgcgag cgtttcgtgc gcttccttgc tgatcgaacc gaaggacatc
       61 gcaccggtgt tgaatcgttt gacgatttct ttggccgatt ccacttcttc gatcggaatc
      121 ggccggggaa ggaacttgaa atccagcaaa ccgcgcaagt tcgaccgctg cttactctcg
      181 tcgttcacaa tctgcgaaaa ttcggcgtag gtcttcgggt cgttcgagcg gctggcgtgc
      241 tgcagcttgt agatcgtctc cggattccag ttatggtgct caccctgtat gcggtagtgg
      301 atctcgccgc cgaaatcgag ttggcgaatc gccgacggct cataggccac gctgtgacgg
      361 cgcaaggttt cctcgccgat gtcgcggatg ccgactcctt ccacgcgcga cggggtcccc
      421 gtgaagtagc ggtcgatcag ttcgcgattc aacccaatcg cctcgaagat ctgcgcaccg
      481 caataggact gcaccgtgga gatccccatt ttcgagaaga tcttgagcag acccttgttg
      541 atggccttga tgaattttcc ttcggccgtc tgtgcgtcga gtccttccgg cagatagttg
      601 tcgcgctcca tgtcgacgag cgactcgaac accagatacg gattgattgt cccggcgcca
      661 tacccgatca aacaggcgaa ctgatgcaca tcgcgcggct cgccggtttc gaggatcaag
      721 cccgcttcgg ttctcgtaca ctcacgaacc aggtgatggt ggactgcgga gataccgagc
      781 aaacttggga tcggagccca ctgttcatcg acgccacgat cgctcaaaat gaggaaggtg
      841 tagccttcct tgatcgcctg cgaggcctgc cggcacagct cgtcgaccgc ctcgccaagc
      901 ccatccggac cttcggcgac gcggaacaac atgcggagcg tcttgctctt gaagtgcgga
      961 tcggaaatgc cgcggatctt ttgcaaatcg gcattcgtga gaatcggctg ctgcaccttg
     1021 atccggcggc acgattccgg cgattcgtcc atcaagttcg gcttcgggcc gatgttggtc
     1081 acgagcgaca tcacaagctg ttcgcgaatc gggtcgatgg gaggattcgt cacctgcgcg
     1141 aagagctgct tgaagtactt gaacagaagc tgtggccgat cggacaggac cgccaatggg
     1201 gtgtcggtgc ccatcgagga gatcgcttct tcgcccgtca cgatcatcgg cgtgatgacc
     1261 atcttgagtt cttcgaccgt gtaaccgaag gcttgctgtc gctgacggat cgtcgggtgg
     1321 tcgggctgcg gcacattcag cggatccggc aactcatcca gcgagacacc atactgcgtc
     1381 acccaactgc gatagggctt gcggccgacg atatcggcct taatctcctc gtcatcgatg
     1441 atacggccct gcaccgtgtc gacgaggaac attcgacccg gttggagtcg gcctttcatg
     1501 cggatatctt tcgcgtcagc cggtaagacc cccgcctcgg atgccaggac cacgagtccg
     1561 tcggtcgtca cttgatagcg gcagggacgg agtccgttcc ggtccaacgt cgcgccgatc
     1621 atcttgccat cggtgaaaca gacggcggcc ggtccgtccc acggctccat catcgccgcg
     1681 tgatactgat agaagccccg gcgatccaaa tccatctgcg catttgccac ccatggttct
     1741 ggaatcagca tcatcatggc atgcggcagc gaacggcccc cgagcaacag aaattcgagg
     1801 gtgttgtcca gacaagccga gtcgctttga ttctcggaga caatcgggaa cagcttctcc
     1861 atgtccttcc cgaacagctc ggaatgaaga cggccttggc gggccttcat ccaattgaca
     1921 ttccccttca gtgtattgat ctctccgttg tggcacacat accgataggg atgggcccgc
     1981 ggccaggtgg gaaaggtatt cgtgctgaag cgtgagtgca ccaaggccag tgcgctcacc
     2041 atccgttcat cggtgaggtc ttggtagtaa gccgacattt gatgcggcaa cagcagtccc
     2101 ttgtagacaa tggtataggc cgacaagctg gatacgtaga agtgttctcg cccttgaatg
     2161 gccgactctg ccacggcctt ttcaacccgt ttgcggatca catacagctt tcgctcgaac
     2221 cgggcctcat tcaaggcatc gcgagcgatg aagatttgcc gcatgaacgg ttcggtcgtt
     2281 cgcgcttggg caccgatctg atcactcttg accggcacgt cgcgccagcc gagcaagcgc
     2341 aggccttcct ccacaaggat ctcggtgaac agcttctcgc acagccgtct ggagtctgcg
     2401 ttgggcggca aaaacaactg ccctactccg tactcgccga cttccggcaa tgacaggccg
     2461 gcatcccctg cgacccgctt gaggaatgtg tgcgggacct gcaaaagaat tccggctcca
     2521 tctcccgtgt aaggatcact gccttgcgca cctcgatggg taagattctc caatacttga
     2581 agtcctttgc ggacaatatc atgggatttc ttccctttaa tattgacgac aaagccgatc
     2641 ccgcaagagt ccttctcgtg ttgaggatcg tagagccctt gtcgacgggg aaacccagga
     2701 atagtcatgt acctatctcg ttcaaagaat gagagtttac ctagacatgg ggggatagag
     2761 ccagtgagaa ccttgggtgg attttccccc atagccctca gggtgaacct cacccgattg
     2821 ttaggatgga aacttactgg atgggtcatt cttctgtcaa ggttacgagg cttataccgc
     2881 tttcaattgc ttgactttat ttcctcggct gccataccat ccgccgcatg gcacaaggtc
     2941 cagcgatcgc aaccctgcac agcatgtcgg atgtgtggga cgcctaccgt ggtgaactgg
     3001 acggagtgga agatcgagtc aagaagaacc tcgactccag cgtgaccctg gtcaatacgg
     3061 tcgccgccca catcttgagc ggtggcggca aacgcatcag acccctgctc ctcctcttgt
     3121 cagctcgtct ctgcggctat cctgggaccg agcaccatca gctcggcagt atcgtggaat
     3181 tcatccatac cgccactctg ttacacgatg atgtcgtcga tgacgccgat ctccggagag
     3241 gcagaagaac cgcgcgcaag gtttggggaa accagatcag cattctggtc ggcgactacc
     3301 tctataccaa ggccatgtgc aaagtcgtcg agttccagag tcaaggcatc aatgaagtcc
     3361 tagccgaggc ctgcaagaaa atggcggaag gggaggtcct tcagctgtac tataatggga
     3421 atccttcaat gccggagatc gattatatca agatcgtcga gcacaagacc gccggcttga
     3481 ttgccgcggc ctgccgcatg ggggccattc tggccgaggc ttctgaaacg caacagatgg
     3541 cgctgttccg cttcggccag tacctcggca tcg
//
LOCUS       DATMTI010000355         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_98146, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000355 DATMTI010000000
VERSION     DATMTI010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98146"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..477
                     /locus_tag="VN039_05910"
     CDS             127..477
                     /locus_tag="VN039_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWV45546.1"
                     /translation="MGTEPRLGSKVFYKIGEVSQLTKLPAYVLRFWETQFTFLRPKKS
                     RGNQRLYVQRDVETVLEIKRMLYEEGHTLEGVKRYWVRRGRAASRRLRPKEVAKKLRG
                     DLQVILKILDAHSS"
     gene            551..627
                     /locus_tag="VN039_05915"
     tRNA            551..627
                     /locus_tag="VN039_05915"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:585..587,aa:Pro,seq:ggg)
     gene            738..1550
                     /gene="surE"
                     /locus_tag="VN039_05920"
     CDS             738..1550
                     /gene="surE"
                     /locus_tag="VN039_05920"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWV45547.1"
                     /translation="MRILVTNDDGIHSPGITALSKALAAIGEVWIVAPDRERTAVAHA
                     VTLHKPLRLHQLGPRTYAVNGTPVDCVNLALLKIMPKPPVIVVSGINKGVNLGDDVMY
                     SGTVSAAMEGTILGIPSMAVSQEGMDTFRFDVGASYAVRVARLVLAHGLPEETLVNVN
                     VPNRPRREMKGVRVTCLSRRRFHNPIIEKLDPRGRKYYWIAGERISWSRSKDADHEAI
                     EEGFVSITPIRLDTTHYGVLDQFRAWEPIMNRGIRKSSPARPTIGRTGKLGA"
     gene            1550..2188
                     /locus_tag="VN039_05925"
     CDS             1550..2188
                     /locus_tag="VN039_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWV45548.1"
                     /translation="MGGLIEAVISELSRFVIACISRFGYGGILFTMAVESACIPLPSE
                     IIMPFSGYLVMTGQFTMLGVTLAGAVGNVLGSIAAYYAGVWGGRPFAERYGPYFLVSH
                     RDLDIADRWFAKYGEAAVFFSRMLPVVRTFISLPAGIAKMNFPRFVVFTFVGALPWCY
                     LLAYIGLRMGEQWEHLRDYFHQFDIVIGLVLAAAVGYFLWSHWPKRRTSAGA"
     gene            2193..>2615
                     /locus_tag="VN039_05930"
     CDS             2193..>2615
                     /locus_tag="VN039_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWV45549.1"
                     /translation="MLKLFNTLTGRQEGFEPIEPKKVRMYVCGVTVYDYCHIGHARSA
                     LVFDMLRRYLEYSGYAVTFVKNFTDVDDKIIKRANEQGVSCDAVTAKYIQAYHEDMGK
                     LRIRAATGEPKATEHIADIVQLTERLIKKGLAYVVDGDV"
BASE COUNT          571 a          652 c          794 g          598 t
ORIGIN      
        1 catcgtgccg tgagtcctcg gcggccttgc gggcggtctt tttgatcatc ctgcgtaata
       61 aagcagacgg gtcaacagcc agctcggctt cgtcgacaga ctacaaggca caacgtgagg
      121 ccggtcatgg gaactgaacc caggctgggg agcaaggtct tctataaaat cggcgaagtg
      181 agtcaattga cgaagcttcc cgcgtatgtg ctccgtttct gggaaactca attcactttt
      241 ttaagaccca aaaagagtcg aggaaaccaa cgcctctatg tacaacgaga tgtcgaaacc
      301 gtattggaaa tcaagcgtat gctgtatgag gaaggacata cccttgaagg cgtcaagcga
      361 tactgggtac gccgtgggcg ggctgcgtca cgtcggctgc gcccgaagga agtggccaaa
      421 aagttgagag gggatctcca agttattctc aagattctcg acgcgcattc atcatgaacg
      481 aagagtaggt ttaccgaagg aagtcctctg ctcatcggca agatgggtat ccgcggagca
      541 atgaacgtgt cggggcgtag cgcagcccgg tagcgcactc gcttggggtg cgagtggtcg
      601 tgggttcaaa tcccgccgcc ccgaccaatc tccaaagtgt tgagtgagga gggactgagg
      661 actgagcggc tggacctacc ggctcagcct tgatccaggg agctgcctcg agcagctctt
      721 tttgttgaag gtcgattatg cgcattctcg tgaccaacga cgacggtatc cactcgccgg
      781 gaatcacagc cttgtcgaag gcgcttgctg cgatcggtga agtctggatt gtcgctccgg
      841 atcgggagcg tacggcagtc gctcacgccg tgacattgca caagccattg cgtcttcatc
      901 aactgggccc acggacgtat gcggtgaatg gaaccccggt ggattgcgtg aatcttgcgc
      961 tgcttaaaat catgcctaaa ccgcccgtca tcgtcgtgtc cggcattaat aaaggggtca
     1021 atcttggcga cgatgtgatg tactcaggca ccgtgtcggc ggcgatggaa ggaaccattc
     1081 ttggcatacc gtccatggcg gtatcccaag aagggatgga cacatttcgc tttgatgttg
     1141 gtgcaagcta tgcggtacgc gtcgctcggc ttgtccttgc tcacggtctg cccgaggaga
     1201 cgctcgtgaa cgtgaatgtt cccaatcggc cgcgacgtga aatgaagggt gtgcgggtca
     1261 cctgtctcag ccgaaggcgg tttcataatc cgatcatcga aaagcttgat ccgcgcggac
     1321 ggaagtatta ttggattgcc ggcgagcgga tatcgtggag ccgcagtaag gacgctgatc
     1381 acgaggcgat cgaagaaggg tttgtctcca tcacgccgat ccgtctggat acaacgcact
     1441 atggagttct ggatcaattc cgtgcgtggg agccgattat gaaccggggc atcaggaagt
     1501 cctcacccgc tcggccaacg atcggacgta cagggaagtt aggggcgtga tgggaggcct
     1561 gatcgaagcc gtcatcagtg agttgagtcg atttgtcatc gcgtgtatct ctagatttgg
     1621 ctatgggggg atcctcttca cgatggctgt cgaaagcgcc tgcattccgc ttccaagtga
     1681 aatcatcatg ccgttttcag gctatctggt catgaccgga cagttcacga tgctcggggt
     1741 cacgctggcc ggcgcggtcg gcaatgtgct cggttcgatc gcggcctatt atgcgggcgt
     1801 ctggggtgga cgaccatttg cggagcgcta tggaccgtat tttctggtct cgcaccgcga
     1861 tttggatatc gccgatcgct ggttcgcaaa atacggagag gcggccgtct ttttcagcag
     1921 aatgctcccg gtggtacgca cgttcatttc gcttccggcc ggcattgcga agatgaattt
     1981 cccacggttt gtcgtcttca catttgtagg ggcgctgccc tggtgctatt tgttggccta
     2041 tatcggcctt cgtatgggcg aacagtggga acatcttcgg gattactttc atcaattcga
     2101 catcgtcatc ggacttgtcc tggctgcagc cgttggatac ttcctctggt ctcactggcc
     2161 aaaacgccga acgagcgcgg gagcttaaac acatgctcaa actgttcaat acgctgaccg
     2221 ggcggcaaga agggtttgag ccgattgaac cgaagaaagt acgcatgtac gtctgtgggg
     2281 tgacggtcta tgactattgc catatcggtc atgcgcgcag tgcattggta ttcgacatgc
     2341 tccggcggta cttggaatac agtggctacg ccgtgacgtt cgtcaagaac ttcacggatg
     2401 tggacgacaa gatcatcaag cgggcgaatg aacaaggcgt ttcttgtgat gccgtgacgg
     2461 ctaagtacat tcaggcctac catgaggata tgggcaaact gagaatccga gctgcgacag
     2521 gagagcccaa ggccacggag catatagccg acatcgttca gctgacggaa cgattgatca
     2581 aaaaggggct ggcgtacgtg gtggatgggg atgtg
//
LOCUS       DATMTI010000356         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_140815, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000356 DATMTI010000000
VERSION     DATMTI010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140815"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VN039_05935"
     rRNA            complement(<1..1493)
                     /locus_tag="VN039_05935"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1942..2017)
                     /locus_tag="VN039_05940"
     tRNA            complement(1942..2017)
                     /locus_tag="VN039_05940"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1982..1984),aa:Ala,seq:tgc)
     gene            complement(2043..2119)
                     /locus_tag="VN039_05945"
     tRNA            complement(2043..2119)
                     /locus_tag="VN039_05945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2083..2085),aa:Ile,seq:gat)
     gene            complement(2326..>2584)
                     /locus_tag="VN039_05950"
     rRNA            complement(2326..>2584)
                     /locus_tag="VN039_05950"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          569 a          768 c          596 g          651 t
ORIGIN      
        1 gcccctgcat ggacaatcca ataagcccat acaagttatg ccatccgtca cttcacactg
       61 gctccggaat atttaccgga ttcccatcga ctacgccttt cggcctcgcc ttaggggctg
      121 gctaaccctg cgcagattag ctttacgcag gaacccttgg tctttcggag agagtgtctc
      181 tcacactctt tatcgctact catgtcagca ttctcacttc cgatacctcc agccaacctc
      241 gcgattgacc ttcacaggct tacggaacgc tccgctaccg ctcaactcgc gttgaaccca
      301 tatcttcggc gcatggcttg agccccgtta cattttccgc gcaggatcgc ttgatcagtg
      361 agctgttacg ctttctttaa aggatggctg cttctaagcc aacctcctga ttgtcaaagc
      421 aatcccacat cgtttcccac ttagccataa cttgggggcc ttagatgatg gttagggttg
      481 tttccctttt cacgacggac gttagcaccc gccgtgtgtc tgccggacag ttctcctggg
      541 tattcggagt ttggttagaa ttggtacagc tcgcgccgcc cgcatccatc cagtgctcta
      601 ccccccaggg aatacatccg acgctctacc taaatagatt tcgcggagaa ccagctatgt
      661 ccaggtttga ttggcctttc acccctatcc acaagtcatc cgaatctttt tcaacagata
      721 tcggttcgga cctccagtac atgttactgt accttcatcc tgctcatgga tagatcacct
      781 ggtttcgggt cgtcatacaa cgaactgaac gccctgttca gactcgcttt cgctgcgcct
      841 acacctatcg gcttaagctt gctcgttaca tgaagtcgct gacccattat acaaaaggta
      901 cgccgtcaca ccgcgtgggt gctccgactg cttgtaggct tccgatttca ggatctgttt
      961 cactcccctt gtcggggtgc ttttcacctt tccctcacgg tacttgttca ctatcggtcg
     1021 tagaggagta cttaggcttg gagggtggtc cccccatgtt cagacaggat tgcacgtgtc
     1081 ccgccctact cgagtctctt gctatttgac gcctacggga ctgtcacccg ctatggtcag
     1141 cctttccaga ctgttcagct ttatatcaca agagcactgg cctggtccca tttcgctcgc
     1201 cactactttg ggagtctcgg ttgatgtcct ttcctccggg tactgagatg tttcagttcc
     1261 ccgggtttgc ttaataagcc tatgtattca gcttattata ccttcataac cgccaactca
     1321 tcggcactcc cctaaaggag atagatgaga gagcagtcaa aggtgggttt ccccattcgg
     1381 agattaccgg atcaaagggt gctagcgcct ccccggtact tatcgcagct tgccacgtcc
     1441 ttcttcgcct ctctacgcca aggcatccgt cagaagccct ttaacgcttg atcgttctca
     1501 acaaaactca tgttgtacag gacaattcaa actcaatcgc tttgtctaaa gctccaccct
     1561 agggaggtgg tgagtcgaac tgtccaacat aagtcgttct tgatgcttca gcctatgcgc
     1621 actggaaaga gatgcgcgca taaacctgtt cgaagcatcg ttttgtcaga caatgtcttc
     1681 tcggataacc cctaaagcta tgaacgtgcc ggggataccc ttcatttaca ataacactgg
     1741 gccaattggc ccagacccgt ttgaccagag gttaccctca gaccaaacaa acttgttcgc
     1801 atcgtaagct gtcatgccat tcgccgctca accaaacatg ggagcaaagc gacctagcga
     1861 ccgacgggag cgcaggctgc gcgccgccaa gaggcgaagc ctctgagcgc ccagcacaaa
     1921 accatcttcg ctccgcgaag atggtggagc cagacggagt cgaaccgacg acatcctgct
     1981 tgcaaagcag gcactctacc aactgagtta tggccccatc cccaaggacg gtcgcttaac
     2041 tctggttggc ctggacagac tcgaactgtc gaccttacgc ttatcaggcg tacgctctaa
     2101 ccacctgagc tacaggccat cagggtcaag cgttcaccca cgctttaaag cgcttccggg
     2161 cttacgatgc acaatatcct gattgccgaa accctatgtc ccgaccggat attgtgtatt
     2221 ggaaagagaa acgaagacgg cggcgtcccg catttttgaa gctcaaggct tctgataagt
     2281 gacaatcgaa taagcgtcgt gaaacgcctg gacgattatc cttagaaagg aggtgatcca
     2341 gccgcaggtt cccctacggc taccttgtta cgacttcacc ccagtcgctg accctaccgt
     2401 ggtcgcctgc ctccttttgg gttagcacag cgccttcggg taaaaccaac tcccatggtg
     2461 tgacgggcgg tgtgtacaag gcccgggaac gtattcaccg cggcatgctg atccgcgatt
     2521 actagcgatt ccaacttcac gccctcgagt tgcagaggac gatccgaact gagacgactt
     2581 ttag
//
LOCUS       DATMTI010000357         5747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_311171, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000357 DATMTI010000000
VERSION     DATMTI010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311171"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..560
                     /locus_tag="VN039_05955"
     CDS             348..560
                     /locus_tag="VN039_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45550.1"
                     /translation="MVHDKVAKSKSYTDKNAYAYTYPHGDPYTYADAYRNSHTYADAY
                     ADPHPNDNYSIANTDDNTAHGRLFRL"
     gene            619..1440
                     /locus_tag="VN039_05960"
     CDS             619..1440
                     /locus_tag="VN039_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45551.1"
                     /translation="MRNYLSSIGRNFPQVNGLGTNKWGMVYGVGTASDPVYKLTGSMP
                     ANVANQLKNVGFHAPASFIDKLTGTSDSPFVVVDEATGISVWGAGASKVDSTTINVTS
                     AGYFTHGTNGLDTNSIGNHSDCAAKGAVCTVSRGRIPEAFLITAAQFETAKVNHTGIG
                     HVLEFFWPETDSSGCLFPMAGCEGGNAGWGFEGQRVALDPTLDIVDRAGCDDSDDVLV
                     RTLQQNGGYIGDNAGGTSWVIKAEQGATFTGLTQNGLSGCVTSNDFVATDNGPFN"
     gene            3403..>5747
                     /locus_tag="VN039_05965"
     CDS             3403..>5747
                     /locus_tag="VN039_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWV45552.1"
                     /translation="MRTYLYIDAAPADNVRIMSAAASVGGLAGSVQLTTGRVLRIVDH
                     NGSVVATGSTQVGIGNWVRVELDIFGDASAGTMSARLYLDPDSTTPSETISASSLNTG
                     GSITQLWFGISSTTSASVTYWLGEMAAADTGAVDPTILNPEFLVIVADMQPGAAVNPP
                     PSVVAGKITALSPRPDYVLMPGDLANDGTATQYGFFQTMYGSLMDVIYPVPGNHDWVP
                     GNLTAYDGEFTAEGHSPQHYYSFNTVGGWHIIGMESDAANVGAPNIGTAQYNWLVSDL
                     AANTGKPIIAFWHHPRWGEGTNSTDPGGTGDSVVMQDVWNLLRDYNADLVFTGHIHSY
                     QRFPKFGKTGTADANGIREFVVGTGGSGLFPLKSSGRRTEVDSYQATAYDQLNSQWFG
                     YLKLWTTGTQYSWQFISQNAGVLDSGGPVAVNNAPAVPQAITGSESPAISIAEASSVF
                     VTIASTDTAAISVAESRAISSTLNRTDSVALSIVESRTLALTIISSDSAAVSVTEASN
                     VFNNLTGNESIALSINEARAIAVTQQTTDAVALSISDSSNSFTTVGATDASSLAISET
                     SSLLVIRSTSDTSAISIADASLSNLTLSRTDSTSLGIAEGISTVVNSFSSTDTASVSI
                     TEASAFSSTVVGSETTAINIADSSSVSITNAVSASDTSAVSLTDDSASVIFSGRTDSS
                     SISVSETSLVAVTQPTNDTSTISIADSSALAVITFVSAADTAAISISDNRAVQVTRTA
                     SDSAAITIVDVSQPFTNFPGVQPVAISVTETSTITVTQPAT"
BASE COUNT         1483 a         1517 c         1449 g         1298 t
ORIGIN      
        1 atgtgcactg ggggaagtat aggagattaa atgaaaatca aagcggttct agcaaccatt
       61 ttggtcgtgt gtttgattgt tctcggcttt gaggccatgc aacagtctgc ctcagcgact
      121 aatacaaacc caccgacatc atgctctgcc aagtggcagg gcggtacagt agattatgtc
      181 tattggaccc gtggaaccag tccatcatat tggagaattc aaaaggttgg cgggggactg
      241 gacatccagg tacccggtgt catgcgtgag tacaccgatt actatcgggg aggaaaagac
      301 gtacgttatc gagtagcgtc cataaataac gaaggcgtgc aaagcacatg gtgcacgaca
      361 aagttgccaa gtccaagtcc tacaccgaca aaaacgccta cgcctacacc tacccccacg
      421 gtgaccccta cacctacgcc gacgcctacc gtaactccca cacctacgcc gacgcctacg
      481 ccgaccccca ccccaacgac aattactcca tcgccaacac agacgacaac accgcccacg
      541 ggcgactgtt tcgactatga ggccggggca tacttgtgtt ctgggccagg agcggtagac
      601 acacagaaaa ctacggcaat gcgaaactat ctgtcaagca ttggcagaaa ctttccacag
      661 gtaaatggcc ttggaacgaa caagtgggga atggtttatg gtgtcggtac agcgagtgac
      721 ccggtataca agttgactgg aagcatgcca gccaatgtgg caaaccagtt gaagaatgtc
      781 ggcttccatg caccagccag tttcattgac aaattgaccg gaaccagcga ttcgccattc
      841 gtggttgtag acgaggcgac cggtatatcg gtttggggag caggagcctc taaggtcgat
      901 tcaacaacga taaatgttac gtctgccgga tacttcacac atggtacaaa tggattggac
      961 accaattcca ttggaaatca ctctgattgt gcagccaagg gcgcggtatg tacagtgtct
     1021 cgcgggcgaa ttcctgaggc atttctcatt accgccgcac aattcgaaac tgctaaagtc
     1081 aaccatactg gtattggtca tgtgctagag ttcttctggc cagagaccga ctcaagtgga
     1141 tgcctgttcc cgatggccgg atgcgaaggc ggtaacgcgg gatggggatt cgaggggcag
     1201 cgcgttgctc tggacccaac tctagacatt gttgaccgag caggatgcga cgattcagac
     1261 gatgtgctgg ttcgtacttt gcagcagaac ggcgggtaca taggtgacaa cgcgggcggt
     1321 acatcttggg taatcaaggc agagcaggga gcaacattca ccggccttac ccagaacgga
     1381 ctatccggtt gtgtaacatc aaacgacttc gttgccacag ataatggtcc attcaactga
     1441 gttgacattt tgaacaactg aatatataat ccgagtatct cgttttgagg taggaggatt
     1501 tctaaccgcc ccagagggac gttaattcgt ctgctctggg gcggttttat ttatgtctag
     1561 gttaggaatg gagtaatggc gggagtaatc tcagcagtcg taactgctag ttcatcgaat
     1621 actagttcgt atacttctgg ctccttcacg cccgtagcgg gcgacctgtt ggttgtcgcc
     1681 gttttggcct cggctacaac gggggctgca tcaacatgta caaccacatc aaacggtttg
     1741 acgttcacgc gcgtgttcca agtacttgga gacagcgggg tagaccgcgt ggcctggtat
     1801 gtgtcagacc agttggtccc ttccagcccg acagcaatga ccgtggtggt ctccttcccc
     1861 acagacagtc cgaccggttc tgtcatcact gtctctcttg tcagtggaat gtcaaagacg
     1921 ggtgcaactg ctgtattaca aactgccggt acagacaaca acctgacaat cagtcagact
     1981 cctagtgtaa cgttcggttc tgcaccaggc tcttcaaatg ttgtctttgg tatggttaat
     2041 aacaatacca acccggccgg agtgacacca ccaacaggat ttaccgagat tactaatacg
     2101 ggattcaaca gtccggttaa tggtgtagag ttagcataca aaacgtctgc ggcaccacag
     2161 accatcactt gggggagcac gaccccatcc cgtggagccg tgaccgccat cgagttggac
     2221 gcctcatctc ctggaacacc taagtctggt acagagactc ctgcaatatc cgtaagcgaa
     2281 acaagttcta ttttcgtcac aaatgtaccc ggcggtggtg gtgccacacc agacctttca
     2341 aactcggcag aaggcacgag cgggacaaca gtatcaacat ctaacagtgg tgggccaaac
     2401 caatttgacg cactgaccgg tacgccagcc tacgacaata ctcatgctgc tcatggttcc
     2461 acatcaatga agttcggccc gagccagata agttatgtta tttggcaagg gtcgatagca
     2521 agcccacagg ctaatttcta tacccgtgcc tacctatgga tggatgctct acccgctgcc
     2581 aactctctgg ttataaactt ggtcacgtcc gcaggcgcgg cagtgggaaa gattcagata
     2641 acaactgccg gtgcctggcg cattgtcaat ggtgctggtg gaataactgg tactggtgcc
     2701 gttacggtac caactggaca atgggtaaga ttcgaactcg acgcgaccgg tatatcggga
     2761 actaccggaa cactgactgg tcgtatgtat accacgccag acggcaccac ttctgccgac
     2821 acgattagtg ctggtgcgca aacagtaaac gacctagtgt ctcgcgtatg gttcggaccc
     2881 aacgcatcaa atgcgaatgc aatgtggatt gacgatgttg ccgtctcata caccggtccc
     2941 gttggtccag cggtagccac aggaacactt ggcaccgatg ctgcctcgat ttctgtgtcc
     3001 gagacatctg cggtcgtaga cctgtctgca agcactccgg tcagtagttc ggacacagca
     3061 gcgatttcgg tcagcgatgc ctcatcaata tcagtcataa cggccaagag tgctacagac
     3121 acttctgcaa tatctttgac agagacatcg agtcttaatt cgaccgctcc gcctcttcca
     3181 attgtctcaa acgtcggtgc gggaacgagc ggtacagatg taagcacatt aaacacaggt
     3241 ggcccaaatc agttcgactc gatagtaaag agtggcaccg gtacagaaca attcgaccaa
     3301 ctatatgcac cacactctag cgcatccata aagtatactt ctggaacggg tacggcatac
     3361 tctgtttgga gcaccgcatg gggaacgcac agtaacgcat atatgagaac gtacctgtac
     3421 attgatgcag caccggcaga caatgtgaga ataatgagcg ctgcggctag cgttggaggg
     3481 ctcgctggct ctgtccaact tactaccgga agagttttgc gtattgtaga ccacaatgga
     3541 agcgttgtcg ccaccggaag cactcaggtc ggcataggaa actgggtacg agtagaactc
     3601 gatattttcg gagacgcatc ggccggtaca atgtcggcaa gactctatct cgacccagat
     3661 tctacaacac catcagagac gatatctgct tcatcattga ataccggggg ctcaatcaca
     3721 caactatggt tcggtatatc ctcaacgacg tctgccagcg tcacctactg gctgggagag
     3781 atggcagcag cagacacggg cgcggtggac ccgacaattc tcaatcctga gttcttggtc
     3841 atcgtggcag atatgcagcc gggagcagcc gtcaatccgc caccatctgt tgtcgcaggc
     3901 aagataacag cattgtctcc gagaccagac tatgtgctca tgcctggcga cctggcgaac
     3961 gacggcacgg caacgcagta cggcttcttc cagacgatgt atgggtcatt gatggatgta
     4021 atctatccgg taccgggaaa ccacgactgg gtaccaggaa accttactgc atacgatggt
     4081 gagttcacgg ccgagggaca ctcgccacaa cactattatt cattcaatac tgttggcggc
     4141 tggcatatca taggtatgga gtctgacgca gcaaacgtcg gagcaccgaa catcggtacc
     4201 gcccaataca actggctggt ttcagacctg gcagcgaaca ccggtaagcc gattatcgcg
     4261 ttctggcacc accccaggtg gggagaggga acaaactcta ccgacccggg cggtaccgga
     4321 gactctgttg tcatgcaaga cgtctggaac ctattgaggg actataatgc agacttagta
     4381 ttcacaggac acattcactc ttaccagaga ttccccaagt tcggaaagac aggcacagca
     4441 gacgcgaacg gtatacgaga attcgttgta ggtaccggag gctctggact gttcccacta
     4501 aagtccagcg gtagacgaac agaggtcgat tcgtaccagg caactgcata cgaccagttg
     4561 aactctcaat ggttcgggta cctgaaacta tggaccaccg gcactcagta ctcttggcag
     4621 ttcataagcc agaatgctgg cgtgctcgac tctggcgggc cggtagcggt aaacaatgca
     4681 cctgctgttc cccaagcaat cactggtagt gaaagtccag caatatcaat tgcagaggca
     4741 tcttctgtgt ttgtgacgat agcatctaca gacacggctg ccataagcgt agccgagtcg
     4801 cgtgcgattt catcaacgct gaacaggaca gattcagtgg ccttgtcaat tgtcgagtca
     4861 cgcacgctcg ccttgacaat tatcagtagc gactctgctg cggtcagcgt gacagaggca
     4921 tctaatgtat tcaacaatct gactggcaac gagtctatag cgctgagcat caacgaggcc
     4981 agagccatag cggtaacaca acagacaacg gatgcagtag cattgagcat ttcagactct
     5041 tcaaattcat tcacgacagt aggggcaaca gacgcatcat ctttggcaat cagtgagact
     5101 agttctctgc tggtgattcg ttctacatcc gatacctctg ccatctccat tgcagacgca
     5161 tcattgtcca acttgactct cagccgtaca gactcaacgt ctctcggcat tgctgagggc
     5221 ataagcacag ttgtaaattc attctcttcc acagacacgg cgtcagtcag catcaccgag
     5281 gcgagcgctt tcagcagcac tgtggtcggt tctgagacca cagcaattaa tattgcagac
     5341 tcctcctcag tatcaattac aaacgctgtc agcgcctctg acacgtcggc agtgtctctt
     5401 acagatgatt ccgcatctgt aatcttttct ggacgtacag actcttcgtc catctctgtt
     5461 tccgagacat cccttgtggc agtcactcaa ccaacgaacg atacttctac aattagcatt
     5521 gctgactcaa gtgctctggc agtaataact tttgtttctg ctgccgatac cgccgccatc
     5581 tccattagtg acaatcgagc ggtacaggtg acacgaacag catcagactc cgctgcgatt
     5641 actatagttg atgtatctca accattcaca aatttcccag gagttcagcc cgtagcaatc
     5701 tcggtcacag agacatcgac aatcacggtc actcagccag ccacaga
//
LOCUS       DATMTI010000358         1539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_565189, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000358 DATMTI010000000
VERSION     DATMTI010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565189"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="VN039_05970"
     rRNA            <1..1503
                     /locus_tag="VN039_05970"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          363 a          361 c          486 g          329 t
ORIGIN      
        1 ggtgaatatt cctgtaccgg ttcttgatgc gatgggggga cggagaaggt taggccagcc
       61 gggtgttgga cgtcccggtt caagcgtgta gacgtgcctg gtaggcaaat ccgccgggct
      121 tagtcgaggc gtgtcaacga gggctggctt gccagcccga agtggcggat accctgctcc
      181 caggaaaagc ctctaagctt cagtcgttgg acgaccgtac cgcaaaccga cacaggtggg
      241 cgagctgaat atgctcaggc gcttgagaga actcaggaga aggaactcgg caaattgaca
      301 ccgtaacttc gggataaggt gtgccctcat taggtgatgc cccttgcggg tggagccgag
      361 gagggcagca gagaatcggt ggctgcgact gtttaacaaa aacacaggtc tctgctaagt
      421 cgaaaacgac gtataggggc tgacgcctgc ccggtgccgg aaggttaaaa ggagaggtca
      481 gccgcaaggc gaagctttga attgaagccc cggtgaacgg cggccgtaac tataacggtc
      541 ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc gtaacgatct
      601 gacgactgtc ggcgcgctcc gcccggcgaa cttgtggttc cggtgaagac gccggatgcc
      661 cgccactaga cggaaagacc ccgtgcacct ttactatacc tttgcaatga actatggtgc
      721 ggtctgcgca gcataggtgg gaggctttga actcaggctc ttgggcttga gggagccaac
      781 ggtgagatac caccctgatc gtactgtggt tctaacttac tcccgtaatc cgggagaagg
      841 acattgcaag gcgggtagtt tgactggggc ggtcgcctcc taaattgtaa cggaggcgct
      901 cgaagcttgg ttcacgctgt ttggaaatca gcggtcgagt gcaaaggcat aaaccaggtt
      961 gactgcgaga cctacaagtc aagcaggtgg gaaaccagga cttagtgatc cggtggttct
     1021 gtatggaagg gccatcgctc aacggataaa aggtacgccg gggataacag gctgatcgga
     1081 gtcaagagtt catatcgacg ctccggtttg gcacctcgat gtcggttcat cgcatcctgg
     1141 gggtgcagaa gctcccaagg gttaggctgt tcgcctatta aagcggtacg tgaactgggt
     1201 tcagaacgtc gcgagacagt tcggtcccta tctgtggtgg gcgcaggaga tttgaggggg
     1261 cttgccctta gtacgagagg accggggtga acagacctct tgtgattcag ttgtcacacc
     1321 cgtggcacag ctgagtagcg aagtctggtc aggataagtg ctgaatgcat ataagtacga
     1381 aaccttccca aagatgagat ctccctgaag agccgtgaaa gacgatcacg ttgataggac
     1441 ggatgtgtaa gcgcagtaat gcgttgagct gaccgttact aatagctcgt tcggcttgac
     1501 cataaaatta actctgcggt ttgagagttg cccgtcaat
//
LOCUS       DATMTI010000359         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_353509, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000359 DATMTI010000000
VERSION     DATMTI010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353509"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VN039_05975"
     CDS             complement(<1..444)
                     /locus_tag="VN039_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWV45553.1"
                     /translation="MSHYILFTDLDGCLLDNDTYSFDEARPALAALRSEGIPVILVSG
                     KTRAEIEPLRERLDHHHPFIVENGAAVFVPLNTFDFPLERSRRRSNYHIIELGTPYAL
                     LRDVLRQIEEAVGTPLQGFGDLSVDEVMANTGLSREDALRAMLREF"
     gene            complement(449..1156)
                     /locus_tag="VN039_05980"
     CDS             complement(449..1156)
                     /locus_tag="VN039_05980"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45554.1"
                     /translation="MTSSWEHTLLGPVAALGERVFAILPNVLAMMILLLVGLGAAWGM
                     GHLTERLLRVIGLDRLCDRIGIAAALLRGGIKADPSYIIGRITYWLIVIFATTASLGA
                     LDVAPINQAAQSLLSYIPHLVTAAVIGIIGYLVSNFVSQAVLIAAVNAGLPPARWIAA
                     GTRWGIQLLTVAMALEQLGIAQHIVVVGFGITWGGLVLAAALALGLGGKDLAKDFLER
                     RLAGHSRSQINEDLRHL"
     gene            complement(1169..2479)
                     /locus_tag="VN039_05985"
     CDS             complement(1169..2479)
                     /locus_tag="VN039_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HWV45555.1"
                     /translation="MNTGLIPLTPETEDLLAAVRSADILVGIPSFNNAGTVGHVVRAV
                     GAGLAKYFHGHRAVLVNADGGSSDDTSAIVAHTMVDLEPLFISDRQSTLHRIVTPYHG
                     IPGKGSAFRTIFEIARRLKVKACAVVDSDVRSITPEWMELLLHPIIKEGYDYVAPYYR
                     RHKYDGTITNSIAYPLTRALYGQEVRQPIGGDFGFTGELAQHYLEKHVWESDVARFGI
                     DIWMTTEAITSGAKVCQSFLGAKIHDTKDPAADLSSMLRQVVGALFALMEAHAPSWLP
                     VTDSRKVPLFGFQYEVGVEPVNVNVERMVDLFHLGLTDLHDIWARILSEDALDRLSRL
                     RGVSIRDFRIPDSLWAQIIYDAALAHHQNVLPQEHLLKALTPLYLGKTASFVLDSQGL
                     TSAEAEHLIEALCRTFEKQKEYLVTRWPQSQDAREVIGAAPREA"
     gene            complement(2476..>3316)
                     /locus_tag="VN039_05990"
     CDS             complement(2476..>3316)
                     /locus_tag="VN039_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HWV45556.1"
                     /translation="LARGQSQVIALHDCDIVSYDRQYLARLCYPVANPNLAYEFCKGY
                     YSRVTDRMHGRVTRLFITPLIRSLQLLVGPHPLLSFLDSFRYPLAGEFAMVRDLAWIN
                     RIPGDWGLEVGMLAEVYRNCALRRICQADIADAYEHKHQTLSTHNPDAGLLKMCVDIT
                     KSLFRNLASEGLVLSESTLKTLRATYLQAAQEAISRYENDAAINSLRFDRHEERRAVE
                     VFLRGMTLATDSFLGDPLGVPMISNWSRVAHAVPDIFHRLMDAVEQDHAWDPAAETRQ
                     PVS"
BASE COUNT          726 a          956 c          988 g          646 t
ORIGIN      
        1 gaactcccgc agcatagccc gcaacgcgtc ttctcgggag agccctgtgt tcgccatgac
       61 ttcgtcgacc gacaggtcgc cgaatccttg gagcggtgtg cccaccgctt cttcgatttg
      121 ccggagtaca tcccggagga gcgcataggg ggtcccgagt tcgatgatat gataattgga
      181 gcggcgtcgc gaccgctcca gggggaagtc aaaggtattg aggggcacaa acaccgccgc
      241 gccattctcg acgatgaagg gatggtggtg atcaaggcgc tctcgaagcg gttcgatttc
      301 cgcacgggtc tttccggaga cgagaataac cggaatgccc tccgaacgaa gggccgcgag
      361 cgccggtctc gcctcgtcaa aggaatacgt gtcgttgtcc aagagacaac cgtccaggtc
      421 tgtaaatagg atatagtgtg acatgggttt agagatgccg taggtcctcg ttgatctgcg
      481 accgggaatg tccggccaac cgtcgttcca ggaaatcctt cgccagatcc ttaccgccca
      541 accccagtgc cagcgcggcg gctaggacga ggcctcccca ggtaatcccg aatcccacga
      601 cgacgatgtg ctgggcaatc cccaactgct caagcgccat ggccaccgtc aagagctgaa
      661 tgccccaccg tgtcccggct gcgatccagc gggccggagg caaccccgcg ttcacggcgg
      721 cgatgaggac ggcctgcgac acaaaattcg agacgagata gccgatgatc ccgatcacgg
      781 ccgctgtgac caaatgggga atatacgaca agagcgactg agccgcttga ttgatcggag
      841 caacgtcgag cgcgcccaac gatgccgtgg tggcgaagat cacgatcaac cagtatgtaa
      901 tgcgaccgat gatgtaggac ggatcggctt tgatgcctcc cctcagcagg gccgcggcga
      961 taccgatccg atcgcagagt cgatccaggc caatcacgcg aagcagccgt tccgtcaaat
     1021 gccccatgcc ccaggcggcg cccagaccga ccagcaaaag aatcatcatg gccaggacat
     1081 tgggaagaat ggcgaagact cgttcaccga gagcagcgac tggtccgagt aacgtatgtt
     1141 cccagcttga agtcatgact gactcctttc aggcctcgcg tggggcagca ccgatgactt
     1201 cccgcgcatc ttgagactga ggccaacgag taacaagata ttctttctgt ttttcgaacg
     1261 tccggcagag cgcttcgatg aggtgttcgg cttccgccga ggtcaacccc tgactatcca
     1321 gcacgaatga cgccgtcttt cccaaataga gaggggtcag ggctttcagg agatgttctt
     1381 gtgggagcac attctgatga tgcgcaaggg cggcatcata aataatttgg gcccacagcg
     1441 aatcgggaat gcgaaaatcc cgtatggaga cgccccgaag acgggacagt cgatccagtg
     1501 cgtcttcaga gagaatgcgc gcccaaatat cgtgcaggtc cgtaagcccg aggtgaaaca
     1561 agtccaccat ccgttccaca ttgacattca ccggttcgac tcccacctca tactggaagc
     1621 cgaagagagg tacctttcgc gagtctgtga caggcaacca gctgggggca tgggcttcca
     1681 tcaaggcgaa cagggcaccc accacctgtc gcaacatgga ggaaaggtcg gcggcagggt
     1741 ccttggtatc atgaatcttg gccccgagaa aactctgaca caccttggct ccgctcgtaa
     1801 tggcttcggt cgtcatccaa atgtcgatcc cgaagcgcgc cacgtcggac tcccagacat
     1861 gcttctcgag gtagtgttga gccagttctc cggtgaatcc gaaatctccg ccgatcggct
     1921 gacgaacctc ctgtccgtac agggctcgag taagggggta cgcgatgctg ttggtgatgg
     1981 tcccgtcgta cttgtggcgt cgatagtacg gggccacata gtcgtagcct tctttgatga
     2041 tcggatgaag gagcaattcc atccactcgg gtgtgatgct gcggacgtcc gaatcgacga
     2101 cggcgcaggc tttgactttg agccgccgag cgatctcgaa aatcgtgcga aacgcgctgc
     2161 ccttgcccgg gattccgtga tagggcgtga cgatccggtg gagcgtgctc tgccgatcgc
     2221 tgataaagag cggttccaga tcgaccatgg tatgggcgac gatggccgag gtatcgtcgg
     2281 aagagccccc atcggcattc accaggacgg cacgatgccc gtgaaagtat tttgccaggc
     2341 ccgctcccac ggcgcgaacg acatgcccga cggtgccggc attgttgaag ctgggaatgc
     2401 cgaccagaat atcggcactg cgcactgctg ccaacagatc ttcggtttca ggagtgagtg
     2461 ggatcaggcc ggtattcatg agactggttg ccttgtttcc gctgcggggt cccaggcatg
     2521 gtcttgttcc acggcgtcca tgagccgatg aaaaatatcg ggtacggcgt gggcgacgcg
     2581 gctccagttc gagatcatgg ggacgcccaa cgggtctccg aggaaactgt ccgtcgccaa
     2641 cgtcatgccg cgcagaaaca cttcgacggc gcgccgttct tcatggcggt caaagcgcaa
     2701 actattgatc gccgcgtcat tttcataccg actgatggct tcttgcgcgg cttggagata
     2761 cgtggcccgc aacgtcttga gggtgctttc cgacaggacc agcccttcgc tcgccaggtt
     2821 gcggaatagt gacttggtga tgtccacgca cattttcagc aagcctgcat ccggattgtg
     2881 cgtcgacagc gtttgatgct tgtgctcata ggcatcggcg atatccgcct ggcagatccg
     2941 tcggagcgcg caattgcggt acacttccgc cagcatcccg acttccaacc cccagtcacc
     3001 cggaatacgg ttgatccagg ccaggtcccg caccatcgcg aattcaccgg ccagcgggta
     3061 ccgaaaactg tccagaaatg aaagcagggg atgcggaccg accagcagtt gcaggctgcg
     3121 gatcagcgga gtgatgaaga ggcgtgtcac acgtccatgc atgcgatccg tcacgcggct
     3181 gtaatagccc ttacaaaatt cgtatgcgag gttcgggttg gcgaccgggt aacagaggcg
     3241 agcgaggtac tgacggtcgt agctcacaat gtcgcagtca tgaagagcga tcacctggct
     3301 ctggcctcga gccaac
//
LOCUS       DATMTI010000360         6057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_183915, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000360 DATMTI010000000
VERSION     DATMTI010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183915"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..448)
                     /locus_tag="VN039_05995"
     CDS             complement(65..448)
                     /locus_tag="VN039_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWV45557.1"
                     /translation="MDQETRHTLAVTFRQNAGVTFVKMQGSLSATTAEQGIQEMKKIV
                     DSGAKKVVINLADVDYISSGGIRVLILACKQLNNVQGEMKIAAAKGMVKEALETGGFN
                     LLSRVYGENNQLCNTEEEAVAAFKA"
     gene            complement(644..3385)
                     /locus_tag="VN039_06000"
     CDS             complement(644..3385)
                     /locus_tag="VN039_06000"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HWV45558.1"
                     /translation="MLLGGAAAYVILSIAERMRAPDQESVKMRWLTIGAVAAGLEIWA
                     MHYIGNLAFCPPVPAPQDSALAVLSLLSAAVTGAVAVYLISSHVHSRMRLVSGGMLMG
                     ACMNLTHFSSMLAVHQPIDLQNGVLLFVLSVVGIVVLSIIVLAVGAWNISYGNWRVTE
                     KASAMGLALSGSHLIGMISTYSIPNVTTSAPPPGIEVDLLAVVAVSLSTLLAIIDRQV
                     TAASRLARDSHARMTEAIESVPQWFALFDPDDRLVICNRKYREVVSGRGAEVQLGDSF
                     ESIIRRIAERGDIPAAIGNVESWIQKRLDMHHNPQGLYIQYRSSGEWIQIHERRTHDG
                     GIVFIATDITALKRAEQAAEDAKTRLADSLAVVEAAKARMQEELNVGRDIQQSMLPRV
                     FPAFPDRKELELYAVLEPALEIGGDLYDFFLLDDHRLCFVIGDVSGNGVPAAMFMAMT
                     KIMVKTRASSDPSPASIVTHVNDALSAENDSCMFVTLYLGILNLRDGTLLATNAGHNP
                     PLLKRRDGQFDWLTAQDGPLVGPMPGIAFKESLIQLRPGDELFLYTDGVTEADNRRRE
                     LFGKDRLKAVLDRSRAVSVVDRIGDVMKAVKTFAGDAPQADDITILGIRYLGVAPSDV
                     SVSVFRQTMLNQLAAIPMVQTAFERYVAQWERAKPLIPTFNMALDDLLNNVVQYAFPN
                     DPTEHTIEVEGEVRDECAVLTITDDGIPFNPLTVATPDLSLLLHEREIGGLGIHLVRS
                     MFDEVTYHRNVGRNVLTVKKKFVSEPPASLKRSDQTGIRAVEVGEKSLQPGQDSTSMA
                     MSVTSHRNGDVMIVTPQNRFDTNSAPEIERILTEHIERGKRRIVLDLSRISYISSIGL
                     RVILKAVMTMTRAGGRVVLSGGNDHVGTVLQLSGTLMMSLHASTLEDAVAKVRETGS"
     gene            complement(3541..4602)
                     /gene="lepB"
                     /locus_tag="VN039_06005"
     CDS             complement(3541..4602)
                     /gene="lepB"
                     /locus_tag="VN039_06005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWV45559.1"
                     /translation="MRYPPRIHEREEYRLNGKILSRLSRILDRSSNRVAICLLVTCNG
                     FWAGCGTPPIVQSDRDSESQAVFHQLAGLEMASDRLYESIADQADMAGFSHQLEVGSD
                     LASPFTSSEKERIAQSFVRSLKVMLFDLAPTNFWEDHLVQYYASTLPAPEARRLVEGY
                     GSEILIPTSKMQELRRDFVTDRLPDLLPAVRAQSHLFHISNEAMVPALLPGDHVITHR
                     TAYHAAEPQRGDVVVYRYPDEDGTLFLHRVIGVPGDRIAIRHQVVSVNEDTVTEPYVQ
                     HTDRSNMVGNVRDNFGPVTVPPDAYFLLGDNREESMDSRFLGPISKGYILGQARFIYW
                     SVDPVTRTPRWDRLNQPVH"
     gene            complement(4730..5923)
                     /locus_tag="VN039_06010"
     CDS             complement(4730..5923)
                     /locus_tag="VN039_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HWV45560.1"
                     /translation="MNRIYDVIVVGSGPAGSCAAWRLAQAGVAVAVLEKAVLPRYKTC
                     GGGLVGRALRALPVDVRHAIEQECHTAQLNFLPAGLSFTTHRQTPIVSMTMRSQFDFA
                     ILSAAQAAGAAVHEQCAVENVSFHDDFVTLVTSAGSMRAKFIVAADGALSTVARIIGP
                     ADGRVLIPAIEYEVTVPHDRLDRFHGTARFDFGLLPQGYAWAFPKNDHLSIGVLSTAQ
                     RGSDLKQAVAHYLDLIGCGSVTKVERHGFVIPIRPRRGPFIDKRILLVGDAAGFADPV
                     TGEGISFAIQSGLLAAQALIDGKLEEESIRNTYSGSVAETILPELQRGRVLARLLYDF
                     PRMRSWAFAQQGQRLCEAVTDIMAGKRQYRDLRFKPQTLLRLLSPKWFRNSVHRLARP
                     RDDQT"
BASE COUNT         1322 a         1799 c         1665 g         1271 t
ORIGIN      
        1 aaccgatttt gaggcagaga ttctttttcg gcaccgaggc cgaatgagag gtggatcagg
       61 cgctctaggc tttaaaagcg gccacagcct cttcttccgt attgcacaat tgattatttt
      121 ctccatatac gcgggacagc agattaaatc caccggtttc aagagcttcc ttgaccatcc
      181 ccttcgctgc ggcaatcttc atctcccctt gaacgttatt aagctgcttg caggccagaa
      241 tcaggacgcg gataccgccg ctgctgatgt agtccacatc agccaggttg atgacgacct
      301 ttttcgcacc tgaatccacg atttttttca tctcctggat tccttgttcg gccgtggtgg
      361 cggaaaggct accctgcatc ttgacgaagg tcactccggc gttctggcga aaggtcaccg
      421 ctaaggtgtg tcgggtctct tggtccatgg ttacactcct ttttcttcgc ttcttcctca
      481 atcatctcgt tcacaacggc tgtcctcttc gcaccgggtt cgccgctccc acagtgcgcc
      541 ccatcataat accatttata cattccctcc ttccatccgg acgatcctaa agcgggcgtc
      601 aatgagtcac cgaggggctc gcacatcggt caatcgaaag gtgtcatgag cctgtctctc
      661 ggaccttcgc gacggcgtct tccagcgtgg aggcatggag gctcatcatc aaggtcccac
      721 tcaattggag cactgttccg acgtggtcgt tccctccact gagtacgact cttccacctg
      781 ctcgggtcat ggtcatcacg gctttcaaga tcacccgcag tccaatggat gaaatgtacg
      841 aaattcgaga gagatccagt acgatccgcc gtttcccacg ctcgatgtgc tccgtcagga
      901 tgcgctcgat ctccggcgcg ctgttcgtgt cgaaacggtt ctgcggggtg acgatcatca
      961 catcaccgtt tcgatgtgat gtgacgctca tggccatgct ggttgagtct tggcctggtt
     1021 gtagcgactt ctctcctact tcgaccgccc tgatcccggt ctgatcggaa cgtttaagag
     1081 aggcgggcgg ctcggagacg aacttctttt tcaccgtcaa gacgttgcgc cccacgttgc
     1141 gatggtacgt cacctcgtca aacatggagc gcaccaagtg aattcccaaa ccgccgatct
     1201 cgcgttcgtg caggagcagc gacagatccg gcgtggcgac cgttaatggg ttgaagggaa
     1261 tcccgtcatc cgtaatggtc aggaccgcgc actcatcgcg cacttcgcct tccacctcaa
     1321 tggtgtgctc ggtcggatca tttgggaagg catactgcac gacattgttg agcagatcat
     1381 ccagcgccat gttgaatgtg gggatcaacg gcttggctcg ttcccattgc gccacatatc
     1441 gctcaaacgc cgtctgtacc atgggaatgg ccgccaattg gttgagcatg gtctgacgaa
     1501 acacgctcac cgacacatcg gacggggcga cgccgagata ccgtatcccc aatatggtga
     1561 tatcgtccgc ttgcggtgcg tcaccggcga aggtcttcac cgccttcatg acatccccga
     1621 tgcgatcgac caccgagacg gctctggatc ggtccagtac cgctttcaat cggtctttgc
     1681 cgaacaattc gcgccgccta ttgtctgctt cggtgactcc atcggtgtac aagaagagct
     1741 catcccccgg tctcaattgg atgaggctct ccttgaaggc aatgccgggc atcggaccga
     1801 cgagaggccc gtcttgagcc gtcaaccagt caaactgtcc atcccgccgc ttcaacagag
     1861 gaggattatg gccggcattg gtcgcgagga gcgtgccgtc gcggagattc aagatgccca
     1921 gatacagggt cacgaacata caggaatcgt tctccgcgct gagcgcatca ttcacatggg
     1981 tgacgatact ggcgggcgag ggatccgacg acgcccgagt cttcaccata attttggtca
     2041 tggccatgaa catcgcagca gggaccccgt tgcccgacac atcgccgatg acgaaacaga
     2101 gccgatggtc atcgagcagg aagaaatcat acaaatcgcc tccgatttcc agggccggtt
     2161 caaggactgc gtagagctcc agttccttcc gatccggaaa agccgggaat acccgtggga
     2221 gcatactttg ttgaatatct ctgccgacat tcagttcttc ctgcatgcgg gctttcgcgg
     2281 cctccactac cgccaatgaa tccgccaacc gcgtcttggc atcttcggca gcctgttccg
     2341 cacgtttcaa cgccgtaatg tcggtggcaa tgaagacaat gcctccgtcg tgcgtcctcc
     2401 gctcgtgaat ctggatccac tcgccgctgg aacggtattg aatgtagagc ccttgagggt
     2461 tgtggtgcat gtcgaggcgc ttctgtatcc aagattcgac attcccgatc gcagcgggaa
     2521 tgtcaccccg ttcggcaata cggcgaatga tcgattcgaa cgaatcgccg agctggacct
     2581 ccgcccctct ccctgacacc acttcgcgat acttgcgatt acagatgacc agacggtcgt
     2641 ccgggtcaaa cagagcaaac cattggggaa cgctctcgat agcctcggtc attcgggcgt
     2701 ggctatcacg cgccagtctt gacgccgccg tcacttgccg atcgatgatg gccaggagcg
     2761 tcgagagcga tacggcgaca accgcaagga gatctacctc gattcccggc ggcggagcac
     2821 tggtcgtcac gttgggaata ctgtaagtcg aaatcatccc gatgaggtgg gacccggaaa
     2881 gagcaagccc catagcgctc gccttttccg tcacccgcca gtttccatac gagatattcc
     2941 aggctccgac cgcgaggacg atgatgctca gtaccacgat ccccacaacc gacagcacga
     3001 agagcaacac gccgttttgg agatcgatcg gctggtggac cgccagcatg ctggagaaat
     3061 gggtcaggtt catgcaggca cccatgagca ttcccccgga gacgagtcgc attcggctgt
     3121 ggacgtggct gctaatcagg tacacggcca cggctcccgt caccgcggcg gacagaaggg
     3181 aaagaacggc gagcgcggag tcctggggtg ccgggaccgg gggacagaac gccagattcc
     3241 cgatgtagtg catcgcccat atttcaaggc cggctgcgac ggcaccgatc gtgagccatc
     3301 gcattttgac ggattcttgg tcgggcgcgc gcatccgttc ggcgatgctg agaatgacat
     3361 aggcggccgc gccgcccagc agcatcgagg aaacggcaag taagggatcg cagattccaa
     3421 gcacggtgaa cccactcagg taacgtcaat gagcatcgta gacatgatgg caagtgccgg
     3481 gattttggac cattgcggca aggcgtcgtc gaacctgatc cggatatcat ctaccatggg
     3541 tcaatgcacc ggttgattga gccgatccca gcgaggagtc cttgtgacgg gatcaaccga
     3601 ccagtagatg aaccgcgcct gccccagaat gtatcccttg ctgatcggtc ccaagaagcg
     3661 gctatccatg ctctcttccc ggttgtcacc caagaggaaa taggcgtcgg gaggcacggt
     3721 cacaggcccg aagttatcac ggacgtttcc gaccatattg gatcggtcag tatgttgcac
     3781 atagggttcg gtcacggtgt cttcattcac ggagaccact tgatgacgaa tggcgatccg
     3841 atcgccggga accccgatca ctcggtgaag aaagagtgtt ccatcctcgt ccggatagcg
     3901 atagacgacg acatcaccgc gttgaggttc ggctgcgtga tacgcagtcc tgtgcgtaat
     3961 cacatggtct cccggcaaaa gcgccgggac catcgcctcg tttgagatat ggaacagatg
     4021 cgactgcgct cgtaccgccg gcaacaggtc cggcagacgg tcagtcacaa aatcccggcg
     4081 gagttcctgc atcttcgacg tcgggatgag gatctcgctg ccgtacccct cgaccaaccg
     4141 ccgggcctcc ggcgccggca acgttgaagc atagtattgc acgaggtggt cctcccagaa
     4201 attcgtcgga gccagatcga acagcatgac tttgagcgag cgcacaaacg actgtgcgat
     4261 gcgctccttc tccgacgaag tgaacggact ggcgagatca gacccgacct ccaactgatg
     4321 gctgaaccca gccatgtcgg cctggtccgc aatcgactcg tacaaacgat ccgacgccat
     4381 ttcgaggccg gccaactgat gaaaaacagc ttgcgactcg ctgtcacggt cggactgaac
     4441 tataggagga gtgccacacc cggcccaaaa tccattacat gtcacaagca gacaaatggc
     4501 gacgcgattc gaagatctat caagaatgcg ggatagccgg gagagaatct tgccgttcag
     4561 ccgatactcc tctcgttcat gtatccgggg aggataccgc accgggcctc gataggaaag
     4621 gccactcggg cagaccgtac gcgacgcgta tttcaacaac acgttcgaaa aattgaatag
     4681 tgccggccaa gatgctgcag caagactccc catccctgct cggcctgtat caggtctgat
     4741 catcgcgcgg ccgagcaagc ctgtggaccg agtttctgaa ccatttcggc gatagcagcc
     4801 ggagcagggt ttgtggcttg aacctcaggt ctcgatactg ccgtttccct gccatgatgt
     4861 ccgtcaccgc ttcacagaga cgctgtccct gttgtgcaaa tgcccatgac ctcatgcgtg
     4921 gaaagtcgta cagcagtctg gccaacactc gccctctttg caactcagga aggatcgtct
     4981 cagccactga tccgctgtag gtgtttcgta tggactcctc ctccagtttc ccatcaatca
     5041 aggcctgtgc cgccagcagg ccgctttgaa tggcaaacga aatgccttcc ccggtcacgg
     5101 gatcggcaaa gccggcggca tcaccgacca acaggatgcg cttgtcaatg aacggtcctc
     5161 gtcgcggtcg gatgggaatg acaaagccat ggcgctcgac tttcgtgacg gaaccacagc
     5221 cgatcaggtc gagatagtgc gccaccgcct gtttgaggtc gcttcccctc tgcgccgtcg
     5281 acagaacacc gatcgacaga tgatcatttt tgggaaaggc ccaggcatac ccctggggaa
     5341 gtaaaccaaa gtcaaatctc gcggtgccgt gaaatctgtc cagccggtca tgcggaaccg
     5401 tcacttcgta ctcgatcgcc ggaatgagga cacgcccgtc tgccggacct attattcgcg
     5461 cgactgtgct gagcgctcca tcggctgcca caatgaactt ggcgcgcatt gagcctgcac
     5521 tcgtgactag cgtgacgaaa tcgtcatgga acgagacatt ttctacagcg cactgttcgt
     5581 gaacggcagc tccggctgct tgagcggctg aaaggatggc aaagtcaaac tgagatcgca
     5641 tcgtcatgga gacgatggga gtctgtcgat gcgtcgtgaa ggacaatcct gcaggcagaa
     5701 aatttagctg cgcagtatga cattcctgtt caatcgcgtg gcgcacatcg accggcagcg
     5761 cccgcagcgc tcgtccaacg agaccgccgc cacaggtctt gtaccgcggc agcacggcct
     5821 tctcgaggac ggcaaccgcc actcccgcct gagccagacg ccacgcggca caagacccgg
     5881 ccggtccgct gccgaccaca atgacgtcat agatgcggtt catgcttcgc gcatatggcc
     5941 atttcaagga aggtccggcc taccttttct acctctagaa cccatctgac gcaatgtggc
     6001 tattggagat cgagcataat ccagagaggg ggcgtcagat aataggcagc gacagac
//
LOCUS       DATMTI010000361         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_820510, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000361 DATMTI010000000
VERSION     DATMTI010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820510"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1730)
                     /locus_tag="VN039_06015"
     CDS             complement(<1..1730)
                     /locus_tag="VN039_06015"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HWV45561.1"
                     /translation="MPQTTNMEDVMGDLWLRRRQGRTVVSAVVLSVMLVVVAGCGGGD
                     SGGGTPAPGTPTAGTVVGQVSSLANGAPVSGATVKTDAGTTTTAADGKFSVPAPAGDR
                     TLLHVEANGFAEAFPVTQVRSGQSTNLGVKLVPTGATATVSVATGDTVSVPNSTARVT
                     IPADGLVPKTGGMPAGSVTVSLTPINPSLDTNLMPGGFNGISTGGGTAQPIESFGAML
                     VDIRDNGGNRYTLAQGKTSTIRIPLGTKSANPPATVPLWFFDETTGVWKEEGMATLQG
                     TAPNQYYEGTVAHFSYWNADIVTERIFVTGCVKDANGQPVANALVQTEGLDYTGTAVD
                     VTAADGTFSVAMRKSSRAKLGLFEFDLQTFNVVPISNTVTVGPSATDIHLSNCLVNRP
                     GQLTITTTGLPGGNVGAAYNQTLAAAGGVPGYVWSLNSGSNPLPAGLELNPSGVISGT
                     PTAAGTTTITVKVTDSAGGTATKTFTLTIAAPNMQPLSITTAALLAGTVGTAYNAILT
                     ATGGTGAKTWSISSGILPAGLSLNAASGGISGTPTTAGTSTFTVQVQDSGTPQQSAQQ
                     QLALAINPSGG"
BASE COUNT          398 a          603 c          530 g          411 t
ORIGIN      
        1 cctccgctcg gattgatcgc gagagcaagc tgttgctggg ccgactgctg cggtgtgcca
       61 ctgtcctgca cctggactgt gaatgtggac gtaccagccg tcgtcggcgt gccgctgatg
      121 cctccgctgg cagcattcaa cgacaatcct gccggcaaaa tcccggagct aatgctccag
      181 gtctttgcgc cggtgcctcc cgtcgctgtc aatatggcgt tgtaggctgt gcccacagtt
      241 ccggcgagaa gtgccgcagt cgtgatcgac agcggttgca tgttcggcgc agcgatggtc
      301 agagtgaatg tcttggtcgc agtcccaccc gctgaatcgg tcaccttgac ggtgatcgtc
      361 gtggtgccgg ccgccgttgg agtgccggaa ataacacccg agggattcaa ttccagtcca
      421 gccggcaagg gattcgaccc gctgttcaaa ctccacacat aaccaggcac cccgccggcc
      481 gcagctaggg tttgattgta cgccgctccg acattgcctc cgggaaggcc cgtcgtcgtg
      541 atcgtcagtt gaccaggcct attcacgagg caattggaca gatgaatgtc tgttgccgat
      601 ggtccgacag tcaccgtgtt cgagatcggc acaacattga aggtctgcag atcgaattca
      661 aagagaccga gcttcgcccg gctgcttttg cgcatggcca ctgagaaagt accatcggct
      721 gcggtcacgt cgacggccgt gcctgtataa tccagaccct ccgtttgcac gagggcattg
      781 gcgaccggtt gcccattggc atccttcaca cagccggtga caaagatccg ctccgtaacg
      841 atgtcggcat tccaatagct gaagtgcgcg accgttcctt cgtaatactg attcggagcc
      901 gtaccctgta aggtcgccat cccctcttcc ttccacaccc ccgtcgtttc atcgaagaac
      961 cagagtggaa cggtggcggg agggttggcg gacttcgtcc cgagggggat acggatcgtt
     1021 gaggtctttc cttgcgccaa agtgtagcgg ttccccccat tatctctgat gtcgaccagc
     1081 atcgccccga agctttcgat cggctgagcc gtcccaccgc cggtggagat cccattgaac
     1141 ccaccaggca ttagatttgt gtccagggac ggatttatgg gagtcagtga aacagtgaca
     1201 gacccagccg gcatacctcc ggtcttcggc accagtccat cggcagggat tgtgacccga
     1261 gccgttgaat tgggcactga gacggtgtca cctgtcgcca ccgacaccgt tgctgtcgcg
     1321 ccagtcggca caagcttcac acccagattg gtcgattgtc ccgatctgac ctgtgtgacc
     1381 ggaaacgcct cggcgaatcc attcgcttcc acatgaagaa gtgtgcggtc tccggcagga
     1441 gcagggacgc tgaatttccc atcggccgca gtggttgtag tcccggcgtc tgtcttgacg
     1501 gttgccccac ttacgggtgc accgttcgct aacgaggaaa cctgcccgac caccgttccc
     1561 gctgtcggtg ttcccggagc cggcgtgcct ccaccggagt cgccacctcc acacccagcc
     1621 acaaccacca gcatgacgct caggacgacc gcgctaacaa cagttcgacc ttgtctcctc
     1681 cttaaccaga gatcgcccat cacatcctcc atgtttgtcg tttgcggcat cacctccgtg
     1741 acgaaccttc atctactgaa aagacagaaa gcaccagccc gactggcaca cacagtgttc
     1801 gacttaatcc atacagcaaa aatatcctga cctcccggct acagatatgc cgacagatgc
     1861 tctccaccac agcgaaacta tttctggtat tcggttgtgc aaggcgaact gatgaggaga
     1921 actagcggcc ttaaagaaaa ga
//
LOCUS       DATMTI010000362         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_820538, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000362 DATMTI010000000
VERSION     DATMTI010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820538"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..2355
                     /locus_tag="VN039_06020"
     CDS             310..2355
                     /locus_tag="VN039_06020"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWV45562.1"
                     /translation="MPGLTPLAAPSLIPIPSPQLPKPVIGVSSPSFSFSARQGEGSPT
                     MQTLTVTNIGQGTLNWNATATAAWLTLGPASGKDTGAITVTATAGSLPIGIYNATITL
                     SAAGATAVTIPVNLTVESAPIPPTIGTSPATLSFTAVQGGSISSSQTVSISNTGGGLL
                     SWLASEDASWLGISPTSGTGNGSVTVTTTPGSLTAGTYNTLVTINAANAAPVSIPISL
                     TVTAPLATVALSPSSLTFSGVQGGANPAGQTVVVNSNGNWAASSSATWLTLSPASGNG
                     NGSIAANVALSSAVVGTNNATITVTSGGVARTISVTLTMSVASLTASPNSVTYTATQG
                     AANPSAQTIAIGSNGAWTVSDNVSWLSISATSGTGNGSITAAALTGSLTAGTYNALIT
                     VSSTSATPVSIPVTFTVRAPSPAITVSPTSLTLTGIQGGTNPASQTVVVNSNGNWAAS
                     SSATWLTLSPASGNGNGSIAANVALSSAVVGTNNATITVTSGGVARTISVTLTMSVAS
                     LTASPNSVTYTATQGAANPSAQTIAINANGMWSASDNASWLSLSPMSGSNNGTITASV
                     NTATAFQGNNATTITVKSGGITRTVNVTLTLNAPSSSSVTLTWNANTESDLAGYRVYR
                     ATSSGTYGAPITTIQGNTTSYIATGLQFGTTYFFVVTAFDIAGNESAYSNEVSKSIF"
BASE COUNT          583 a          753 c          621 g          529 t
ORIGIN      
        1 ttcttccatt tgcaaagggc atctaagttg ctcattgctt cctagccttg tgcaaaacca
       61 acaggtcgta atgtgctttg gaggacttcg atcatgcgcc aaaagattca gcgattgttg
      121 ttcctctcgg ccggtacgag catgttgttg ttggtttgtg agcccatgat tccatcgcta
      181 cagccagcat tagcagcgac tagtccggat acggtcacag ttcagcagaa caactctcaa
      241 ggaaaatctc agatagccag tccgatgcaa acccgaccaa cagttccaat gcgcaggctc
      301 atgtctgaaa tgccgggact aacaccactc gctgcgccgt cgctcatccc tattccgtct
      361 ccacaattgc caaagcctgt tataggagtg agttccccaa gcttttcttt cagtgctcga
      421 caaggagagg gcagtccgac aatgcaaact cttaccgtta caaacatagg ccagggaaca
      481 ttgaactgga atgcgacggc taccgcagca tggttgactc taggtccagc ctccggcaag
      541 gacactggtg caatcacggt aacggccacg gctggctccc tccccatcgg catttacaat
      601 gcaacaatta ccctgagcgc tgctggtgct acagctgtca ccattccagt caacctaacc
      661 gtagagtctg cccctatacc ccctaccatc gggacaagcc cagccactct ttcatttaca
      721 gccgtgcagg ggggtagcat ttctagctcg cagactgtca gcatttccaa caccggtggc
      781 ggactcctta gctggttggc cagcgaggac gcttcttggc tgggcatctc cccaacctcg
      841 gggaccggca atggttcggt cactgtaacc actactcccg gctcattgac ggcaggcact
      901 tacaataccc tggttaccat caatgcagcc aacgcagccc ctgtttccat ccccatctct
      961 cttacagtca ctgccccctt ggcgactgtc gcgcttagtc cgtcatcgtt gacgttctcg
     1021 ggggttcagg gaggggccaa tccggcaggc caaaccgtgg tggtcaattc gaacggcaac
     1081 tgggcagcca gtagcagcgc cacctggctt actctcagtc cagcgtcagg caacggcaac
     1141 gggtccatcg cagccaacgt tgccctatcc tccgcggttg tcggcacgaa caacgccacg
     1201 atcaccgtaa cgagtggtgg cgtggcgaga acgatttccg ttacgctaac catgtcagtc
     1261 gcctcattga cggcttcccc caacagcgtg acctacaccg ctacgcaggg agcggcaaac
     1321 ccttccgccc agaccatcgc catcggttca aatggcgcgt ggacggtaag tgacaatgtt
     1381 tcctggttaa gcatctcggc aacctcagga accgggaatg gttcgatcac tgctgccgcc
     1441 ctcaccgggt cactgactgc agggacttac aacgctctga ttaccgtcag ctcgaccagc
     1501 gccactccag tctctatccc cgtaaccttt accgttaggg cgccttcacc agccattact
     1561 gtcagcccaa catcgttgac actcactggt attcagggag ggacgaaccc agcgagccaa
     1621 accgtggtgg tcaattcgaa cggcaactgg gcagccagta gcagcgccac ctggcttact
     1681 ctcagtccgg cgtcaggcaa cggcaacggg tccatcgcag ccaacgttgc cctatcatcc
     1741 gccgttgtcg gcacgaacaa cgccacgatc accgtaacga gcggtggcgt ggcgagaacg
     1801 atttccgtta cgctaaccat gtcagtcgcc tcattgacgg cttcccccaa cagcgtgacc
     1861 tacaccgcta cgcagggagc ggcaaaccct tccgcccaga ccatcgccat caatgcaaat
     1921 gggatgtgga gcgcgagcga taatgcttcc tggctatcgc tcagtcctat gtcgggctcc
     1981 aataatggaa ccatcacggc gagcgtcaac accgctaccg cgttccaggg gaataatgcg
     2041 accaccatca ctgtaaagag tggcggcata accagaaccg tgaatgtcac cctgacgctg
     2101 aacgctccat cgagttcatc cgtcacattg acttggaatg ctaatactga aagcgatcta
     2161 gcgggatata gagtgtatcg agcaacttca tcaggaacct acggagcccc cattacgacg
     2221 atccaaggta atacgaccag ctacattgca acaggactcc aattcggcac gacgtatttc
     2281 tttgtcgtga cggcctttga cattgcgggg aatgaaagcg cctattcaaa cgaagtcagc
     2341 aagagcattt tttgattcac gcgagtgatg acgggaattg gtacgcttcc cacaaacaag
     2401 gtatcggtaa ggagagcgtt ctgagagatg acgacggctt ctttcgaagc agcgcgtaac
     2461 tagcaggatg ttgagttgaa aggttg
//
LOCUS       DATMTI010000363         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_607754, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000363 DATMTI010000000
VERSION     DATMTI010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607754"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1203
                     /locus_tag="VN039_06025"
     CDS             58..1203
                     /locus_tag="VN039_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45563.1"
                     /translation="MPSPIFVVDSSPAVRRLVEQISTPEGFEVVGFQDGPAALEAARR
                     NSPSLIIADYHLDNMTFSGFCKEINKLDNLTETYLISLVNPADRPDENLLRTLGVKAF
                     LKKPFQSEDLLGVLKSLQQKQTDVSNGKSLKRRVWPPTSTSTDSDDEAIDHSTSGEGQ
                     EEGMGQPGNSQSTSPTPPIAAAAPEDAMKGLFDHLLQSMTTRSQQSLAELLPRMIDEK
                     LATHVRPIVQKEVQAQLGSILSQEYLTTIIQPVVSQALPALIRKELEANEPIIRQTVA
                     EMAKVAIGESIDRLVKDQVESGIHQHLPTVVREQVGMVDQLVKDELQRAVVKQAPLLA
                     DDLVRAAIGQTIEQAIQRIVPDVAEQHIQAELRRLIETEEASQSSQT"
     gene            1464..>2232
                     /locus_tag="VN039_06030"
     CDS             1464..>2232
                     /locus_tag="VN039_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWV45564.1"
                     /translation="MIRRQLDKTYDPKAVEDRWSQEWLSRGYFHASPEQPGQPYCIVI
                     PPPNVTGSLHVGHALNHSLQDILIRWRRMQGRNTLWLPGTDHAGIATQNVVEKQLMAE
                     GLSRESLGRERFVERVWQWKATSGNTIVNQQKQLGESCDWDRLRFTMDEGLSKAVLEV
                     FVRLYEDGLIYRGERLINWCPRCLTALSDIEVEHEEVKGKLYHLRYPLADDPNTGLIV
                     ATTRPETMLGDTAVAVHPDDPRYRHLIGKKVRLPLTTR"
BASE COUNT          536 a          636 c          594 g          466 t
ORIGIN      
        1 tgacaaagaa tacccccctt gctagcttcg aagaaactaa aaaaatggag ttttttcatg
       61 ccttctccga tcttcgttgt ggacagcagc cccgcagtga gacggctggt ggaacagatc
      121 tcaaccccgg aagggtttga ggtcgttgga tttcaggacg gaccagccgc ccttgaggcc
      181 gctcggcgaa actctccatc gctgatcatt gcggactacc atctcgacaa catgacgttt
      241 tctgggtttt gcaaggagat caacaagctg gacaacctga cagaaaccta tctcatttcg
      301 ttggtgaatc cggcggatcg accggacgaa aacctccttc gcacgttggg tgtcaaagct
      361 ttcctgaaga aaccgtttca atctgaggat ttgctcggcg tgctgaaatc tcttcaacag
      421 aaacaaactg atgtctcgaa cggcaagagt ctgaaacggc gcgtctggcc gccgacttcg
      481 acttcaaccg actccgatga tgaagcgatc gaccactcga caagtggcga aggccaagag
      541 gaaggcatgg gccagcctgg caactcacag agtacatcac caacaccacc aatcgccgct
      601 gcggcccccg aagatgctat gaagggcctc ttcgaccacc tcttgcaatc gatgaccacg
      661 cgtagccagc agagtcttgc cgagctgctt cctcgtatga tcgatgagaa attggcaacc
      721 cacgtccgac caatcgttca gaaagaggtg caagcacagc tcgggagcat cctctctcag
      781 gaatatctca caacgatcat tcagcccgtg gtctctcaag ccttgcccgc actcatcaga
      841 aaagaactcg aggccaacga gcccatcatc cggcaaactg tggccgagat ggccaaagtt
      901 gccatcggag agagcatcga tcgactggtc aaggatcagg tcgaatccgg aatccatcaa
      961 cacttgccca cggtcgtgcg ggaacaggta ggaatggtcg atcagttggt caaagatgag
     1021 cttcaacgtg cggtcgtaaa acaggccccg ctactggcag acgacttggt gagagccgca
     1081 attggacaaa ccatcgaaca ggccattcag cgaatcgtgc ctgatgtcgc cgagcagcac
     1141 atccaggccg aactcagacg tctgatcgag actgaagaag cgtcacagtc ctctcagacc
     1201 taacttttct tgccccgttt ccctcgtcga gccttggcca ggcctttcat ccgtttgaca
     1261 ttcttccggt gtttctttcg cgtcttccga tccttctcac gacctcgtgc catggtcctc
     1321 ctaccgcgat gtgtttaaag atctctgtgt gggcgactgt atagcacatg ctttataagg
     1381 ccacaagcag agactcctgc cattttctgt gcgagacgga cgtgctaaga tgccggacct
     1441 aacgcttaga gtccggtgga gcaatgatac ggcgtcaact tgataaaacg tatgatccga
     1501 aagccgttga agaccgatgg tctcaggagt ggctctcgcg cggatacttc cacgcctcac
     1561 cggaacaacc gggccaaccg tattgtatcg tcatcccgcc accgaacgtc acgggatcgc
     1621 tccatgtcgg ccatgcgctc aaccattcgc tacaagacat cctgattcgg tggcggcgca
     1681 tgcagggtcg aaataccctc tggcttcccg gaaccgatca tgccggcatc gccacgcaaa
     1741 acgtggtgga aaagcagctg atggccgaag gtctgtcacg ggagtcactg gggcgggagc
     1801 gattcgtcga acgggtctgg caatggaaag caacgtcggg taacacgatc gtcaatcaac
     1861 aaaagcagct gggagaatcc tgcgactggg accgtttgcg atttacgatg gacgaaggtt
     1921 tgtccaaggc cgtccttgaa gttttcgtgc ggctgtatga agacgggttg atctaccgtg
     1981 gcgagcggct catcaattgg tgtccgcgct gcctgacggc cctctcggac attgaagtgg
     2041 aacatgagga ggtgaaaggc aaactctatc atctccgcta tccgttagca gacgacccga
     2101 acaccggttt gatcgtggcc accacgcggc ctgaaacgat gctgggggac acggccgtcg
     2161 ccgtgcatcc ggacgatcct cgatatcgcc atctgatcgg caagaaagtc cgcctgccgc
     2221 tgactacacg tg
//
LOCUS       DATMTI010000364         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_396238, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000364 DATMTI010000000
VERSION     DATMTI010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396238"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VN039_06035"
     CDS             complement(<1..835)
                     /locus_tag="VN039_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45565.1"
                     /translation="MADPTRPSKIPDEQNFRAVHDWCQLAVQEGNAFLQSQSGYDQVD
                     DIIKCIMGTVYEDKLRPNSISQLNLNHMGKVGLDLASSLTDIKPFWEYQVSNKRFEQQ
                     AEMGQKLSSSWWTRRNIDLKFCDVIKYCIAAGSGIAHLTYNSDTQDLDLLAEDPRDVL
                     PIRPSDMISIQNSFGVVVRKERTVNYLRHMYPEFANRITPDRDGSYAALARKANLQAK
                     TAGLNLVSGFMSNLYASLGGRPNAAPLTVPVCDVYTIYVHDDSVNMTGSERIMGDASA
                     NW"
     gene            complement(835..1215)
                     /locus_tag="VN039_06040"
     CDS             complement(835..1215)
                     /locus_tag="VN039_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45566.1"
                     /translation="MKRMVDGRCGTCGNEQENLYLDEDEVSTCASCGGVLEQIWWRRP
                     RGPAQWDDRTSVMVHVNPATGDVRYPGRHDAKLKDGYERQYLRSLPDVNRFEREHKVV
                     NHTMHYDSNGRALDDTHNGQRMTH"
     gene            complement(1212..1436)
                     /locus_tag="VN039_06045"
     CDS             complement(1212..1436)
                     /locus_tag="VN039_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45567.1"
                     /translation="MAKIMTIADLFAPVSTKAETFEQAQNDMDRESWRGRASLLGTPG
                     SGNEGVGREDGGTRTIGPESFVGRKGNVSK"
     gene            complement(1436..1849)
                     /locus_tag="VN039_06050"
     CDS             complement(1436..1849)
                     /locus_tag="VN039_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45568.1"
                     /translation="MAITPVRNVPTVKVSNLRLGEDVFDTFSERAIKKGHEVETEIRR
                     HLEATKAYNASAPIYLDDETRNTLSQLAGRSIASPADLVAWARRLASLQIGEVHIELD
                     ERLATRLDTRRFGKAWPDFLRRIVTESLEEKVGMR"
BASE COUNT          378 a          552 c          605 g          492 t
ORIGIN      
        1 accagttggc ggaggcatcg cccatgatcc gctccgatcc ggtcatgttc acgctgtcat
       61 cgtggacgta gattgtgtaa acgtcgcaaa cagggacagt aagaggggcg gcattcgggc
      121 ggcctccgag tgaggcatag aggttggaca tgaagccgga gacgaggttc aggccagcgg
      181 tcttggcttg caagttggcc ttccgggcaa gggctgcgta cgaaccgtca cggtctggcg
      241 tgatgcggtt ggcaaattcg ggatacatgt gacgcagata gttgaccgta cgttccttgc
      301 gaaccacgac gccgaatgaa ttctggatcg agatcatgtc cgacgggcgg atgggcagaa
      361 cgtcgcgggg gtcttcggct agaaggtcta ggtcttgcgt gtcggagttg tacgtcagat
      421 gggcaattcc cgatccggcg gcgatgcagt acttaatcac gtcgcagaat ttgaggtcta
      481 tgttacggcg tgtccaccag cttgatgaga gcttctgtcc catctccgcc tgctgctcga
      541 agcgtttgtt cgagacctga tattcccaga acggcttgat gtcggtgagg gaggatgcaa
      601 ggtcaaggcc gaccttgccc atgtggttca ggttgagctg gctgatcgag ttgggacgga
      661 gtttgtcttc gtaaaccgtc cccatgatgc acttgataat gtcgtcaacc tggtcgtatc
      721 ccgattgtga ttgaagaaaa gcgttccctt cctgcaccgc gagctggcac cagtcgtgga
      781 cagcacggaa gttttgctcg tcaggaatct tggatggtcg tgtcgggtcg gccatcagtg
      841 cgtcatccgt tggccattgt gcgtatcgtc aagcgcacgt ccgttagagt cataatgcat
      901 cgtatggttc acaaccttgt gttcacgctc aaaacgattg acatccggca gggaacgtaa
      961 atattggcgt tcatagccat cttttaactt tgcgtcgtgg cgaccggggt agcgaacatc
     1021 gcccgtggcg ggattcacat gcaccatcac gctcgttcgg tcatcccatt gtgccgggcc
     1081 acggggacga cgccaccaga tttgttcaag cacaccacca cacgaggcgc acgtactgac
     1141 ctcgtcctcg tcgaggtaaa gattctcctg ctcgttcccg cacgttccgc atcgtccatc
     1201 caccattctc ttcacttgct cacgttccct ttcctgccta cgaagctctc cgggccgatt
     1261 gtgcgcgtac ctccgtcctc gcgtccgacg ccttcattcc ctgatccggg agttccgagc
     1321 agtgacgcac gtccccgcca cgactcacga tccatatcgt tctgagcttg ctcgaacgtt
     1381 tccgccttcg tgctgacggg ggcgaacaga tcggcgattg tcataatctt tgccattagc
     1441 gcattcctac cttttcttcc agggattccg tcacgatacg acggagaaag tcgggccacg
     1501 ccttgccgaa acgccgggtg tcgaggcgtg tggcaaggcg ttcgtcgagt tctatgtgca
     1561 cctcgccaat ctggagtgat gcgaggcgtc gggcccaagc cacgagatca gcgggcgatg
     1621 cgatcgaacg tcctgcgagc tggctgaggg tgtttcgtgt ttcgtcgtca agatagatcg
     1681 gggcggaagc gttgtatgcc ttcgttgctt caaggtgacg gcggatttcc gtctctacct
     1741 cgtgaccttt cttgatcgcc cgttcggaga aggtatcgaa tacatcttcg cccaggcgca
     1801 ggttgctgac ctttacggtt ggaacgttac gtacaggtgt gatcgccatc gtcgtgaatc
     1861 ctcttgggga gagtttcagg ctggcaggtg gggcgtgtca atggggggac ggtgcggcgg
     1921 ggctgggcgt gtcgtggcat gctcaccaat tgctgaacat ctcattccac tgcgtcatca
     1981 tctcgtcata ggtcatgtcc gaatgctgtg cgtcgggcac gttcacc
//
LOCUS       DATMTI010000365         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_396284, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000365 DATMTI010000000
VERSION     DATMTI010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396284"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VN039_06055"
     CDS             <1..1247
                     /locus_tag="VN039_06055"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HWV45569.1"
                     /translation="YSTGGTRPLSIDTLISDGRLRSLLDVRDTTVPDLEASFDRLSAT
                     LANAVNGIHRQGYGLDGSTGLDFFGPPSVSTRAMIANQGAAAINGSAITANSLLTFHD
                     YEIRFSSGSAYSIVDTTTGAVVRGNYTGTAITSPSSDNPLSIVTGTNDTLTVTVDGVA
                     SGTIALGGAASPGLAYTSGTALAQEVQDKINADATLQAAGKTVAVTYDTTTNRLVITS
                     NDTTASSAVNVTGGTARASLGLSAGTSTPATGTYTSPATLTFDGLSVTLTGAAAAGDV
                     FKVDSYSHAARDMTVSLTNPASVAASSTRAGIPGNNTNLLSMVALQQQRFASLDDGTL
                     NDAYRAVAGKIGIAAQTADRENEAKETLKDQIETLRAQVSGVSMDEELVNLMKFQRGF
                     EAASRLVKLTDEMFQTLLSIKP"
     gene            1261..>1758
                     /gene="flgL"
                     /locus_tag="VN039_06060"
     CDS             1261..>1758
                     /gene="flgL"
                     /locus_tag="VN039_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HWV45570.1"
                     /translation="MRVTELQTFGVLANDLARARARALELQEQVSTGKLVRQPSDDPT
                     AFNHIALDKASLAAIDQWQRNIGFGQTRLNLSDNMLSQASETLSRVQELAVQFRNDTN
                     GAADRTAGAKEVRQLFAQLQQSANTELNGQPIFTGTSTHGRATGLAITTPMTLTNGSN
                     DTLMVT"
BASE COUNT          388 a          512 c          499 g          359 t
ORIGIN      
        1 gctattctac ggggggtacg cgtcccttaa gcatcgacac actgatctcg gatggccgct
       61 tacgcagcct cttggatgtt cgtgacacta cagtcccaga tttggaagcc tcgttcgacc
      121 gactgtcagc aacgctggct aacgccgtga atggtattca tcgacaaggc tatggactcg
      181 acggatcgac cgggctcgat ttttttggtc ctccgtcggt atccacgcgc gccatgatcg
      241 ccaatcaagg agccgctgcg atcaatggca gtgcgattac tgcgaacagt ctactcacgt
      301 ttcatgacta cgagatccgc ttttcctccg ggtccgccta ttccatcgtg gatacgacga
      361 ccggcgccgt tgtcagaggg aactataccg gcacggcgat cacgtcacct tcgtcggaca
      421 atccgctgtc tatcgtgacg ggtacgaacg atacactcac ggtcaccgtc gatggcgtgg
      481 cgtccggcac gatcgctctg gggggcgctg caagtccggg tttggcatac acctccggta
      541 cggctctcgc gcaagaagtg caggacaaga tcaatgcaga cgccacgcta caggcggccg
      601 ggaaaaccgt ggcagtgacc tacgacacca cgacgaaccg tctggtcatc acgtcgaatg
      661 atacgacggc ctcatcggcc gtcaatgtga caggcggcac ggctcgagcg tcgctcgggt
      721 tgagtgctgg caccagtacc ccggcgaccg gtacatatac aagtccggcg actctgacct
      781 tcgacggcct cagtgtgaca ttgaccggcg cggcagcggc cggagacgta tttaaggtgg
      841 actcgtacag tcacgctgct cgcgatatga cggtttcact cacgaatccg gcatccgttg
      901 ccgcctcttc cacacgagcc ggtattcccg ggaacaatac caatctcctt tcgatggtcg
      961 cacttcagca gcaacgattc gcgagcctgg atgacggcac cttgaatgat gcttatcggg
     1021 ccgtcgcggg gaagattggc attgccgcac agacggccga ccgcgaaaat gaagcgaagg
     1081 agacgttgaa ggaccagatc gagacgttgc gtgctcaggt gtccggcgtc tctatggacg
     1141 aggaactggt gaatctgatg aagttccaac gggggttcga agccgcgtct cgtctggtaa
     1201 agctcaccga cgaaatgttc caaacgctgt tgtcgatcaa accttagcgt cgaggcttct
     1261 atgcgggtga cagagctaca aaccttcggt gtgcttgcaa atgacttggc tcgggcgcgc
     1321 gcccgagccc tggaactcca agagcaagtg tcgaccggta aattggtgcg ccagccgtcc
     1381 gatgacccca ctgcattcaa tcacatcgcg ttggacaagg cctcgctcgc tgcgatcgat
     1441 caatggcaac gtaatattgg ttttgggcag accaggctta acctctccga caacatgctg
     1501 agtcaggctt cggaaacctt gtcccgcgtg caagagcttg ccgtgcaatt tcgtaatgat
     1561 acgaacggcg ccgcagatcg gacggccggt gctaaagagg tgcggcaatt gtttgcacaa
     1621 ctccaacagt ctgccaatac agaactgaat ggtcagccga tttttaccgg cacgagcaca
     1681 cacggacggg ccacaggact tgcaatcacg acgcccatga ctctgacgaa cggcagcaat
     1741 gataccctca tggtgact
//
LOCUS       DATMTI010000366         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_56189, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000366 DATMTI010000000
VERSION     DATMTI010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56189"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VN039_06065"
     CDS             <1..617
                     /locus_tag="VN039_06065"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWV45571.1"
                     /translation="PSPVVDDKLRREIGRVAVEAVKAAHYRNVGTVEFLLDKDRQFYF
                     MEVNTRIQVEHPITEMVTGIDLIKEQIRLAAGHPLSIRQQDVILTGHSLECRINAEDP
                     EKFTPSPGVITKYSPPGGFGIRVDSAMESGSIVVPHYDSMIAKLITHGRDRQESAARM
                     KRALGEFIIEGIKTTIPLHRRILDDPDFQKGHVSTTFLERFLTS"
     gene            779..2059
                     /locus_tag="VN039_06070"
     CDS             779..2059
                     /locus_tag="VN039_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV45572.1"
                     /translation="MLDVSSTQRMRRLGFIISIVAIGIAIGGYVFFNGERKPPIRYRT
                     AAVERGQVVSVVSATGTINPVVSVQVGTQVSGMIKSLHADFNSRVKAGDTVAIIDPEP
                     FKARREQAAGNLEMARSNVARSKADLAQRKRELDRVQSLLPQQFVSQNDVDVALTNFQ
                     SAEAQLRVAGAQVKQAEAALNAAELELKYTVIRSPVDGIVVARNIEVGQTVASSFATP
                     NLFLIALDLTNMQVDTNVSESDIGGMTEGKEAVFTVDAYPGVSFAGTIKQVRLAPINV
                     QNVVTYNVVISVDNKELRLKPGMTANVAIVVAQRDQVLKVPNAALRFMPPKAEGDRRG
                     LEGQATKAEGGRPIHAGGAAGQSRTVWTQVESGDLVSLSVQTGISDGIATEVLSGGLT
                     EDDSVVVGIEQPFGERKSSELPPGFGNQRRPRSR"
     gene            2174..>2412
                     /locus_tag="VN039_06075"
     CDS             2174..>2412
                     /locus_tag="VN039_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV45573.1"
                     /translation="MNALIICEDIWKVYRVGDVEVQALRGVNLTIRQGEFVAIMGSSG
                     SGKSTLMNILGCLDQPTKGQYRLNSIDVGHLRPDR"
BASE COUNT          564 a          615 c          686 g          547 t
ORIGIN      
        1 ctccctcccc tgtcgtcgat gacaaattgc gtcgggaaat cggtcgcgta gctgtggagg
       61 cggtgaaggc tgcgcattac cgaaatgtgg gcaccgtaga atttttactc gacaaagacc
      121 gtcagtttta tttcatggaa gtcaataccc gcatccaggt ggaacatcca attaccgaaa
      181 tggtgacggg catagatctg atcaaggaac agatccgttt ggccgccggt catccgcttt
      241 ctattcggca acaagatgtg atccttaccg gacacagcct ggagtgtcgt attaatgccg
      301 aagatcctga gaagtttaca ccgtcccccg gcgtgatcac caaatacagc ccgccgggag
      361 ggttcggaat ccgtgtcgac tccgcgatgg agtcaggttc gatcgtcgtt cctcactatg
      421 attcgatgat tgcaaagctg atcacccatg gccgtgaccg acaggagtca gcggctcgca
      481 tgaaacgcgc gcttggcgag tttatcatcg agggcattaa gaccaccatt ccgcttcacc
      541 gtcgaatcct cgacgacccc gattttcaaa aaggccacgt gtcgacgacg ttcttggaac
      601 gattcttgac aagctaagcc cgcaagttct ctcttcgaaa gaacagtctt ttcggtgttt
      661 ctccaccaag tgatctcatt tcttggtaag cgtagagtct catggtaagc tcatcaaagt
      721 gctccccgct tcaagcggga aggtgcatca cctattggtt tcctgatagg atacaccggt
      781 gctcgatgtg tcgagcaccc aacgtatgcg acgtctcggc tttattatta gtatcgtggc
      841 tatcggaatt gcgatcggtg gctacgtgtt tttcaacgga gaacggaagc cccccattcg
      901 ataccggacc gcagcagtcg aacgtggtca ggtcgtttct gtcgtcagcg ctacaggcac
      961 catcaatccg gttgtatccg tccaggtcgg cacgcaagtg tcagggatga tcaaaagtct
     1021 tcacgccgat ttcaactcac gcgtgaaggc cggggatacg gttgcgatca tcgaccccga
     1081 accgttcaag gctcgtcgag aacaggctgc cggcaatctg gaaatggcgc gatcgaacgt
     1141 tgcacggtcc aaagccgacc tggcgcagcg aaaacgggag ctcgaccgtg ttcaatcgct
     1201 gttgcctcag cagtttgttt cacaaaacga cgtcgatgtc gcactcacaa acttccaaag
     1261 cgcggaagca cagttgcggg tcgctggggc gcaggtcaag caagcggaag cggcattgaa
     1321 tgcggcggag ctggaattga aatataccgt cattcggtcg cctgtggacg gtattgtcgt
     1381 tgcgcggaat atagaggtcg gtcaaacggt cgcctccagt tttgccacgc ccaacttatt
     1441 tctgattgcc ctcgatctga ccaatatgca agtcgatacc aacgtgagtg agtcggatat
     1501 aggcgggatg acggaaggga aagaagcggt gttcacagtc gatgcctatc cgggagtgtc
     1561 cttcgctggt accattaagc aggtgcgcct cgcacccatc aatgtccaaa atgtcgtgac
     1621 ctataatgtg gtgatcagcg tagataataa agagttacgt ctaaagcctg gaatgactgc
     1681 gaacgtagcg attgtggtgg ctcaaagaga ccaggtactc aaagttccta acgcggctct
     1741 acgcttcatg cctccgaagg ctgaaggaga ccgccgagga ttggaaggac aagcgacaaa
     1801 agcggagggg ggacgcccga tccacgcagg aggagcagcg gggcagtcga ggactgtttg
     1861 gacacaggtg gaaagcggtg acctcgtttc cctgtcggtc caaacgggta tctcggatgg
     1921 catcgccacc gaagtcctgt ccggcggtct cacggaagat gattcggtcg ttgtcggtat
     1981 cgagcagcct tttggggaaa gaaagagcag cgagctgccg cccggattcg gcaaccagcg
     2041 gcgccctcgt tctcggtgag ccatcggatt gtgtgagaca tacggtgtat gcgatttggc
     2101 ttgccgaacg taatctctta tcactgtccg ccggtcattc atcttcggat aacatctctt
     2161 acaggatagg tttgtgaacg cgctgatcat ttgtgaagat atttggaagg tctatcgagt
     2221 cggcgacgtt gaagtgcagg cgctgagagg ggtgaatctc accatccggc aaggtgagtt
     2281 cgttgcgatt atgggatcga gcggatcagg caaatccaca ctgatgaaca ttctcgggtg
     2341 ccttgatcaa ccgaccaagg gacagtatcg gctgaacagc attgatgtcg gacatttgcg
     2401 gccagaccgg tt
//
LOCUS       DATMTI010000367         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_820807, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000367 DATMTI010000000
VERSION     DATMTI010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820807"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1635
                     /locus_tag="VN039_06080"
     CDS             <1..>1635
                     /locus_tag="VN039_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45574.1"
                     /translation="LISEQYDQGLITEDERYNLTVAAWRGVDSQVTDFLQSKLKDMDT
                     SISVMVNSGARGDISNVKLASAMVGIMVDATNREIELPIRSNYKQGLSSLEQFVATRG
                     ARKGLIDTALKTADSGYLTRRLVDVSQDVFTVEDTAGDDDGFAIYRNETELTMIDFGN
                     RLYGRFTAEEIPGHVGKDELITRQIADAIEADSDVTVVKIQSVLSTKNLRGIPQKSYG
                     IDMATGSLVAKAQPVGVIAAQSVGEPGTQLTLKTFHAGGVAGGDITQGLPRVEELFEA
                     RTPKGQAFMTEVTGLVDVWEDGKKYIVQVTPEAGHVERIPLEGRTVTVKAGSNVKAGD
                     VLATAEGEAKPLVAPFDGVVEVAEDTLVVAANAGAPVRYEIPGNSQLVVKANDVVTAG
                     DRLTIGSLNLHDLMRLKGVEATQRYIINEVLRIYAAQGQDVADKHLEIIVRQMFSRVQ
                     VEDPGDSAFVTGDIVSKAAVVEANAELARDGKELVQYTQLLLGITKVSIWIDSWLSAA
                     SFQDTTRVLISAATSGRADYLHGLKENVIIGRKIPVG"
BASE COUNT          380 a          413 c          477 g          365 t
ORIGIN      
        1 ctcatctctg agcaatacga ccaaggtctg atcactgaag acgaacgata caacctgacc
       61 gttgccgcat ggcgcggtgt cgacagccag gtgactgact tcctgcagtc aaagctcaag
      121 gacatggaca ctagcatatc cgtgatggtt aactctggtg cccgtggtga tatctcgaat
      181 gtcaagcttg caagcgcgat ggtcggtatc atggtggatg ccaccaaccg agaaatcgag
      241 cttccgatcc gatcgaacta caaacaaggt ctcagttcac tggagcaatt cgtggcaaca
      301 cgaggtgccc gtaagggtct gatcgacacc gctcttaaga cagccgactc aggttacctg
      361 acacgtcgtc tggttgatgt gtcacaggac gtgtttacag ttgaagatac tgctggtgac
      421 gatgatggct tcgctatcta ccgcaacgag accgaactga ctatgatcga cttcggcaac
      481 cgtttgtacg gtcgcttcac cgccgaagag attccgggtc acgtcggcaa agacgagctg
      541 ataacccgtc aaatcgctga tgcgatcgaa gccgattcag atgtaacggt agtcaagatc
      601 cagtcagtcc tcagtacgaa gaatctgcgc ggtattccgc aaaagagtta cggtatcgat
      661 atggcgactg gcagtctggt cgcgaaagcg cagccggtcg gtgtgattgc tgctcagtcc
      721 gtcggtgagc caggtacgca gctgactcta aagaccttcc acgctggtgg tgtggctggt
      781 ggcgacatca cacagggtct gcctcgtgtc gaagagttgt tcgaagcccg caccccgaag
      841 ggtcaggcct tcatgaccga agtgactggt ctggtggatg tctgggaaga cggcaagaag
      901 tacatcgttc aggtgacgcc ggaagctggt catgtcgagc gtattccgct tgaaggccgt
      961 accgtaacgg tcaaggctgg atcaaatgtc aaagctggcg atgtgctggc aactgccgaa
     1021 ggcgaagcaa aaccattggt cgctccgttc gatggcgtgg ttgaagtagc cgaagatact
     1081 ctggttgtcg ctgcaaatgc gggcgcgcca gttcgttacg agattccagg caactcacag
     1141 ctggtcgtaa aggcgaacga tgtcgttacg gcaggtgatc gtctgactat cggttcattg
     1201 aatctgcacg acctcatgcg tcttaagggc gttgaggcga cacaacgcta tatcatcaac
     1261 gaagtgctgc gtatctacgc cgcacagggt caggatgtgg ccgacaagca cctggagatc
     1321 attgttcgcc agatgttcag tcgtgtccaa gtcgaggatc cgggtgatag cgcattcgtg
     1381 acgggtgata ttgtttcgaa agccgctgtc gtcgaagcga atgcagaact tgctcgtgat
     1441 ggcaaggaac tggttcagta cacccaactg ctgcttggta tcacgaaggt atcgatctgg
     1501 atcgatagct ggctgtccgc cgcatccttc caggatacga ctcgcgttct catcagcgcc
     1561 gctaccagtg ggcgtgcgga ttacctgcac ggtctgaagg aaaatgtgat catcggtcgc
     1621 aagattccgg tcggt
//
LOCUS       DATMTI010000368         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_735457, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000368 DATMTI010000000
VERSION     DATMTI010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735457"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..774)
                     /locus_tag="VN039_06085"
     CDS             complement(286..774)
                     /locus_tag="VN039_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HWV45575.1"
                     /translation="MTVVIPPNHNNDVFNRVANACSRMFGTAGAFWIACGVVAIWALT
                     GPLFNFSDTWQLVINTGTTIVTFLMVFLIQNTQNRDSAALQLKLAEIIRSTQGAHNAM
                     IDLEKLSQVDLDKIRSLYEHLAERARSTEGQGDSDTGTPAVPEEQTETRVIIEAAGKA
                     HC"
     gene            complement(991..1422)
                     /locus_tag="VN039_06090"
     CDS             complement(991..1422)
                     /locus_tag="VN039_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWV45576.1"
                     /translation="MSPISIKELAHKYAEALNAGEIDRFDDFIADDYINHNPYVKPGL
                     RGIKEFFEGWMRSFPDTEVTVEDVLAEGDMLVGRFTYRATHQGEFLGLPATGRSIRMR
                     SIDIWRVRDGRFVEHWDELNLLEVMHRLGAVSAKGYSTVEI"
BASE COUNT          477 a          575 c          552 g          494 t
ORIGIN      
        1 gcatctacgc cgtctcgaac gcaaagcatt gttaaccgca tttgttcgct catcgtgctg
       61 tcgtagcaag atcgagttag ggtagtatcc ggccatttgt cctccagtca aacacgatca
      121 ttcggcgatc caactcgcag aatccctagc ggccgagagg gtcggggagg tgttcttgca
      181 aagcgacatc aaaacaggtg atggagagaa aaatacgtga agcggtgtaa ttctatggaa
      241 gttgtcgtgt gtggcctcgg tgcagttccg gaaagaagcg aatcatcagc aatgagcctt
      301 cccggctgcc tcaatgatga cgcgcgtttc agtttgttcc tcgggaaccg ccggtgtgcc
      361 cgtatccgag tcaccctgtc cttcggtcga tcgagccctt tccgccaagt gctcgtataa
      421 ggatcgaatt ttatccaagt cgacctggga cagcttttcc agatcgatca tcgcgttgtg
      481 cgctccctgg gtcgagcgta ttatctcggc gagctttaat tgaagggccg cgctatcgcg
      541 attctgcgtg ttttgaatga ggaaaaccat caggaaggtc acgatggtcg tgccggtatt
      601 gataacgagc tgccaagtat cgctgaagtt gaacagcgga cccgtcaatg cccagattgc
      661 cacgaccccg caagcgatcc agaatgctcc ggcggtgccg aacatccgag agcaggcgtt
      721 cgcgactctg ttgaagacat cattattatg attaggtgga atcacgaccg tcattatttg
      781 cggacaggcc aaacatacca actaggtaga tcccttaaaa gcctcgtagt tcggcaatag
      841 aaggctggac gttgttcaat tattcggaag tttttgagga aggtcgcatc gaacgcttgt
      901 ttcaaaaagt cgatcaaccg ctccgcctcc atgcgggctc aatcgccgac ttcaaccaca
      961 tgtcgtttcg cccctgaaag ccggtcggaa ctaaatctcc actgtgctat accccttcgc
     1021 cgagaccgcg cccaatcggt gcatgacctc gagcagattt aactcgtccc aatgctcgac
     1081 gaacctgccg tcgcgcacgc gccaaatgtc gatgctgcgc atccgtattg aacggccggt
     1141 cgcggggagg ccgagaaatt ccccctggtg cgtagcgcga tacgtgaacc gaccgacgag
     1201 catatctccc tcggcgagca cgtcctcgac cgtcacctcg gtgtcgggaa aggaccgcat
     1261 ccagccctcg aagaactctt tgatgccacg gagtcccggt ttcacatatg gattgtgatt
     1321 gatgtaatcg tccgcaatga aatcgtcgaa gcggtctatc tcccctgcgt tgagcgcctc
     1381 ggcgtacttg tgagccaact ccttaatact gattggtgac atgtctcatg ctcctttcag
     1441 tcgagcaagg ggctctgccg gacgggcgag gttttggcat gtttccatca tcgtccgcgg
     1501 accttggtaa ttcggcgaca tgccgtactt cttgcaacgg tgagacgaaa atttttgctg
     1561 agtcaaacag tttctgagtg acgatacaac taggtaaccc tctagaactc ataggtaccg
     1621 tcggcggtca tcccttaaat aaccggatga tttgtccttc ctaaaacaat ttgtcctcat
     1681 agggagccgg gttccctccc ggcatggcaa actcgacgtg gaaaactaga gatgccgcgt
     1741 cgacaacctg cacgcattta gccgcgcgaa gctcgcggcc cacgttggtc gcagcggcgt
     1801 atcggcggtt gcaatagagt gctcaagagt gttcatgaat aagtgggtta ggcccgtatc
     1861 ttgcgatctc ccgtatcttg cggtcagttg actctgttgg tcttccaacg tgggctgcac
     1921 aactaaattc agcaaaaaag gagagactat gtaccggacg agccgcacga gaatgacatc
     1981 tacctttgct gtcattttat ccatgctatc catggcactg ctaggaagcg cctgcactca
     2041 cgccaaaagt cagagtgatc gccgagtcac cgggtcgccg gcctctcagc atcagctc
//
LOCUS       DATMTI010000369         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_227726, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000369 DATMTI010000000
VERSION     DATMTI010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227726"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VN039_06095"
     CDS             complement(<1..322)
                     /locus_tag="VN039_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWV45577.1"
                     /translation="MAGKTLFDKIWESHVVRSEPDGTTLLYIDRQLVHEVTSPQAFEG
                     LKLAGRRPRRPAATLAVPDHNVPTTDRRLGIADHVSALQIQTLEDNCKDFGIALFNMR
                     DIRQG"
     gene            399..905
                     /locus_tag="VN039_06100"
     CDS             399..905
                     /locus_tag="VN039_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HWV45578.1"
                     /translation="MPRLVRRVTTTFVEMSPWNEPRIVEWSRLLLDSHRHWIGKDLIA
                     RIFDTKQQARLLFEASFVVVSHGGEPDPILNYGNQAALDLWELSWDQFIKTPSRLTAE
                     ADDRAERGRMLEQAKLNGYFDGYRGVRISSTGRRFLVEHALIWNVIDSAGNPVGQAAT
                     FSQWTNVF"
     gene            complement(1000..1614)
                     /locus_tag="VN039_06105"
     CDS             complement(1000..1614)
                     /locus_tag="VN039_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV45579.1"
                     /translation="MPIILAVNGIVETYPIKPVGSRNGRAESVGEREHRGQHSPSLDR
                     SALAAQTAYQQQSNDRPYSKPAVLARDLMSAPVFSLPSDSTLLDAWASMSHKGFHHIP
                     VTTMHDTLVGMVSYRDLLHHVPELITANDKRQASGRRLAEIMTSRVISATPATEIREI
                     ARVMLDERIHAVPILDHNRRVVGILAIRDLLQGIANHGPLELWT"
     gene            1982..>2419
                     /locus_tag="VN039_06110"
     CDS             1982..>2419
                     /locus_tag="VN039_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45580.1"
                     /translation="MDKTSLAVLFGLGMVLVGVSGSYALQAGGVETGDLETGTVVGQP
                     FKELTVDAAFYSIDIGNMKTWYPPTTVIDFKTRPGAPVLIKVTNNSTAEHGFQLSAAV
                     NQSGPFVLDTKLVLKPGETRYIGVPTSDLFYAAGNVLEYRCHLH"
BASE COUNT          518 a          608 c          708 g          585 t
ORIGIN      
        1 taccctggcg gatgtcgcgc atgttgaaaa gggcgatgcc gaaatccttg cagttgtctt
       61 ccagtgtttg aatctgcagg gcactcacgt gatcggcgat ccccaaccgg cggtctgtgg
      121 tggggacatt atgatccggc acggcgagtg ttgcggcagg tcgccgtggg cggcgtcccg
      181 cgagctttaa cccttcgaag gcctgagggg acgtcacttc atgaaccaac tgacgatcta
      241 tgtacagcag tgtcgttcca tccggttctg agcgtacgac atgcgactcc caaatcttgt
      301 cgaacaaagt cttgccggcc atctgtgctc ctgtctcgtt aaacgagggc ctcattatac
      361 agaagagtca cagggtcttg acaaggtgtc tgtgaggctt gccccgcctt gtcagacgtg
      421 ttaccaccac gttcgtggaa atgtcgccgt ggaatgaacc gcgtattgtg gagtggagtc
      481 ggctgttgct ggacagccat cgtcattgga taggaaaaga tctgatcgcg cggatattcg
      541 acacaaaaca gcaggcacgt ctgttgttcg aagcatcctt tgtcgtcgtg tcgcacggtg
      601 gggagccgga cccgatcttg aactatggta atcaggccgc tttggacctc tgggaactgt
      661 cctgggatca attcatcaag acgccgtcgc gactcacggc cgaggcggat gaccgtgctg
      721 agcggggaag gatgttggag caagcaaagc tcaacggcta cttcgacgga tatcggggag
      781 tgagaatctc ttcaacgggg aggcgatttc ttgtcgagca tgccttgatc tggaacgtga
      841 tcgattctgc cggaaaccct gtcggtcaag cagcgacgtt ctcacaatgg accaacgtgt
      901 tctgatccag aagggagcat agatttcgat actgccggat tgatcaaggc aacggtcatt
      961 caagggagcc ggacagtcct ccggctgtga aggggggagt cacgtccaga gttcgagggg
     1021 gccgtggttc gcaataccct gcagcagatc tcggatagca aggatgccta cgacacggcg
     1081 attgtgatcc aagatcggaa cagcatgaat ccgttcatca agcatgacgc gggcaatttc
     1141 ccggatctct gtcgcgggtg ttgcagaaat aactcgggaa gtcatgattt cagccaatcg
     1201 ccgccctgag gcctgtcgct tgtcgttcgc cgtgatcagt tccggaacat ggtggaggag
     1261 gtcacgatac gacaccatcc cgacgagggt gtcatgcatc gtcgtgacgg ggatatggtg
     1321 gaatcctttg tgtgacatgc tcgcccaggc gtccagcaag gtgctgtcgg atggaagaga
     1381 gaaaaccgga gcactcatga ggtcccgcgc caggaccgca ggtttcgagt atgggcggtc
     1441 gttgctctgc tgttgataag ccgtttgtgc cgccaaagcg gagcgatcca atgaaggtga
     1501 gtgttggcca cgatgctctc gctctccaac ggattcggcg cggccatttc gtgagccaac
     1561 cggcttgatc ggatacgtct ccacgattcc attgaccgcc aaaatgatgg gcatgcgcag
     1621 ctcctcaact gccgtatcgg aatgttcggg aaatacttaa gctgagaatg ttgccatagc
     1681 cctccagcgt tgttcttgcg gggcattgtc gcctctcgat ctctacagca ttggctgtcc
     1741 gttttgtcct cttcatgcca gattattgct actatttccg tgttccaaat cggttgcttc
     1801 ggttttgagc cctatgctat aaggatcgtc gtgtgatgcg gtgagtgccg acagtcgaca
     1861 aatggtactg agacaataga cgaaaggcgt acgctaccaa aagcctgcac gtcctcaaag
     1921 tttgtcatct aatatttcac aggcctcatt tatctcttct tttttcgcaa ggggggtagt
     1981 catggacaag acgtcgttgg cggtcctctt cggccttggg atggtattgg ttggagtttc
     2041 gggttcctat gcacttcaag caggtggtgt tgagaccggt gatttggaaa ccggcactgt
     2101 cgtcggacag ccctttaagg agctgaccgt ggatgcggcg ttctactcga tcgacatcgg
     2161 caacatgaag acgtggtatc cccctacgac ggtcatcgac tttaagacgc gtccaggcgc
     2221 gccggtgctg ataaaagtga cgaataattc cactgctgaa catggctttc agctgagtgc
     2281 tgcggtcaat caatcgggac cgtttgtctt ggataccaaa ttagtgctca agccggggga
     2341 aacgcgttac atcggtgttc caaccagcga tttgttttat gcagcgggaa acgtccttga
     2401 atatcgttgt cacttgcac
//
LOCUS       DATMTI010000370         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_353985, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000370 DATMTI010000000
VERSION     DATMTI010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353985"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..>1664
                     /locus_tag="VN039_06115"
     CDS             229..>1664
                     /locus_tag="VN039_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HWV45581.1"
                     /translation="MHNGAERHRLLWSGFLRSSAQFPNRAAIDLGGHELTYEQLADRS
                     KSLAATLQANAVCEGVPLTAVLVYRSATAYAAVLGALMAGHGYVPLNRTFPVHRSRLM
                     LQKSMCRSIIVDAASESQLESLLCCIRQSLLIICPERADVTEMAAKFPMHRFIGADQL
                     GKPDQWHPTEVAPDSIAYLLFTSGSTGEPKGVMVSHANVLHYVAYVTNRYRFTFQDRF
                     SQTFDLTFDLSAHDMFVAWESGACLCCPTQKQTIKPDAYLKEAQLTVWFSVPSTAMFM
                     RRLGVLKPNMYPGLRVSLFCGEALPTQTARQWALAAPNSIIENLYGPTELTIACTQYR
                     WDNARLPAECEHAIVPIGQPFEGMDAVVVDEHLHEVPHGISGELVVTGPQLSLGYWQN
                     EEKTRAAFVRIPGKAATYYRTGDRVVRRAADGVLVYLGRRDNQIKILGHRVELGEVEG
                     VLREVSGVEGVVALGWPVTEGRAEAIEV"
BASE COUNT          376 a          425 c          478 g          385 t
ORIGIN      
        1 ttcgctggaa ggaaatcagt cgagtggaac cgcgctacaa tccagaaatt cgacgtcgtg
       61 ttaatcgcca cgaatcattc gtgtgtgaat taccgggaac ttgccaattg ggcatcgtgc
      121 atagtggata ctcggaatgc catggctgcc gtgccagtta attccggtca ggtatggaaa
      181 gcctagctcg gcggcttaac gattctccgc ccatacgcca aaacccaaat gcataacggg
      241 gccgaacgtc acaggttgct gtggagtggc tttcttcgat cgtctgcaca atttcctaat
      301 cgtgcagcga ttgacctggg tggccatgag ttaacgtacg agcaactcgc tgaccgcagt
      361 aagtcattgg cggccactct tcaggcaaac gcagtctgtg aaggagtacc gttaacggcg
      421 gtgctcgtct atcgttccgc gaccgcgtat gcggcggtac tgggagcttt gatggccggt
      481 cacggctatg ttccgctgaa tcggacgttt cctgttcatc ggagtcggct gatgctgcag
      541 aagtcaatgt gccgttcgat tatcgtcgat gcggcatcag agtcgcaatt ggagagtctc
      601 ttgtgctgca tacgccaatc actgctaatt atctgccccg agcgcgccga tgttaccgaa
      661 atggctgcaa aatttcccat gcaccggttc ataggagcgg atcaactggg gaagcccgac
      721 caatggcatc ccaccgaagt tgctccagac tcgattgctt accttctgtt cacatcagga
      781 agcactggtg aaccaaaagg cgtcatggtc tctcacgcga atgtccttca ctatgtagct
      841 tacgtgacga accgatacag gttcacgttt caagatcggt tttcgcaaac gttcgatctt
      901 acattcgact tatcggccca cgacatgttt gtagcatggg agtctggagc ttgcctctgt
      961 tgcccaaccc agaagcagac aatcaagccc gacgcatacc tgaaggaagc gcagttaacg
     1021 gtctggttct cagtaccttc gacggcaatg ttcatgcggc gattgggagt tctcaaacca
     1081 aacatgtatc ctggattgcg cgtgagtttg ttttgcggcg aggcgttacc cacccagact
     1141 gctcggcagt gggcgcttgc cgcccctaat tccatcattg aaaatctcta cggccccact
     1201 gaactgacga tcgcttgtac ccagtaccga tgggataatg cgagattgcc ggctgaatgc
     1261 gagcacgcga ttgtacccat cgggcagcca ttcgaaggaa tggacgccgt ggtggtcgac
     1321 gagcatctgc atgaagtgcc gcatggcatc agcggcgaat tggttgtcac tggtccgcaa
     1381 ttaagtctcg ggtactggca gaatgaagag aagacccgag cagcctttgt ccgaattccc
     1441 ggaaaagcag ctacttacta cagaactggg gatcgcgtag tacgtcgcgc cgcagacggc
     1501 gtgctcgttt atctcggacg acgcgacaac cagatcaaga ttttggggca ccgcgttgaa
     1561 ctgggggagg tagagggcgt attaagagag gtgtcgggcg tggaaggtgt agtcgcgttg
     1621 ggatggccgg tgacggaggg cagggcagag gcgatagagg tttt
//
LOCUS       DATMTI010000371         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_227738, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000371 DATMTI010000000
VERSION     DATMTI010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227738"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VN039_06120"
     CDS             complement(<1..246)
                     /locus_tag="VN039_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45582.1"
                     /translation="MSFQEHRGHVFFGEQRFGASLRLINNRFRLEVSDPEGSFEGGIP
                     EQVDLLHFRTNSSYFSLLHLRRISSKTRLGVSGVSTFS"
     gene            complement(279..1556)
                     /locus_tag="VN039_06125"
     CDS             complement(279..1556)
                     /locus_tag="VN039_06125"
                     /inference="COORDINATES: protein motif:HMM:NF039631.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6035 family protein"
                     /protein_id="HWV45583.1"
                     /translation="MAEKQFKRTLKRAIDLETMLTISSDALMGMPKDDYQVVRRLATR
                     ARLDRRPRYVCGLCGHAVYAPREGRTGQPYWKHHPGAPEDCSWWTGTPSGVDAVSGRQ
                     FHGAQESPLHAKIKNIVGELLTADRRTKPESVVVDAYLITEGGRRRPDVRAVYDEAPL
                     VVEVQLATTQIPIIIQREDFYDSEAYRLLWLTWNFEPPATNGRLLSSFEDIFYSHNKN
                     LFSMDNETVQLSRQRNEVIFRAFWVQENLWQSKLVGLHELNWLSSGRAFAVAPEPPWH
                     EDFLARWLAATGEHGTLRPERGTLLAELADKLSLLDVDDRTLEEVDIDSLINCLLSLL
                     GGHPVGSRQKNLVEVLNTFLHVERRHRFARLIRRFAELAGRKEILATKSVQSKLAVAL
                     KVEQDDPQSSTGRIALALFPEAFALSHKTMERR"
     gene            complement(1717..>2204)
                     /locus_tag="VN039_06130"
     CDS             complement(1717..>2204)
                     /locus_tag="VN039_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45584.1"
                     /translation="DGIERGRRSALPMETFREDVRHPDDLRNVISDPTVLEEVTRLVF
                     NLVCYLNYPERDQEKRLNDPRAHEKVRRAKGKTRKIAEARARQDGCRWINFCGYRTSF
                     DRRAMLGGTAIGHWRRGHWRNQRFGKALTETRLLWIRPTMVGADKDIIDNRPTVYGTS
                     Q"
BASE COUNT          486 a          655 c          569 g          494 t
ORIGIN      
        1 gctgaaggtg ctcaccccgc taacgccgag cctggttttc gagcttattc ggcggagatg
       61 taacagagaa aaatagctgc tattggttcg aaaatgcagc agatccacct gttcggggat
      121 gccgccttcg aaacttcctt ccggatcgga gacttcaaga cgaaagcggt tgttgattag
      181 ccggagtgat gcaccgaaac gttgttcgcc gaaaaagaca tgaccacgat gctcttggaa
      241 agacacttcg atactctctt ttgtttatag aatcgaagtc acctccgctc catcgtttta
      301 tggctcaaag cgaatgcctc tggaaagagc gcaagagcaa tacgtcccgt cgagctctgt
      361 ggatcatcct gctccacctt gagcgccacc gccagcttgc tctggacgct ctttgttgcc
      421 agaatttctt ttctgcccgc gagctcggca aatcgacgaa tgaggcgcgc aaagcggtgc
      481 cgtcgctcta cgtggagaaa agtgttcaac acctctacaa ggttcttctg acgagaccct
      541 accgggtggc caccaagcag ggaaagcaag caattaatca acgagtcgat atcaacctcc
      601 tcgagcgtgc ggtcgtcgac atcgagtagc gatagcttgt cagccagttc cgcaagaagc
      661 gtccctcgtt ccggccgcag cgtaccatgc tcaccagttg cggcaagcca acgagccagg
      721 aagtcctcgt gccaaggagg ctcaggcgcc acagcaaaag cgcgaccgct cgaaagccaa
      781 ttcagttcat gaagaccaac gagcttggat tgccacaaat tctcttggac ccagaaagcg
      841 cgaaaaatga cctcgttccg ttgccgtgag agctgaacag tctcgttatc catcgagaac
      901 aggttcttgt tgtgcgaata aaaaatgtct tcgaatgaac tgagaagccg tccgtttgtc
      961 gccggtggct cgaaattcca cgtcagccac aggagccgat aagcctcgct gtcatagaaa
     1021 tcttcgcgct ggatgatgat tggaatctgg gtcgtcgcga gctgaacctc gacgacaagc
     1081 ggcgcttcat cgtaaactgc gcgcacgtcg gggcgccggc ggcctccttc ggtgatgaga
     1141 tatgcatcga cgactacgct ttccggtttg gtccggcgat ccgccgtgag gagctcccca
     1201 acgatgtttt tgatcttcgc atgcaacgga ctctcttgag caccgtgaaa ttgtcgccca
     1261 ctgacggcat ccacgccgga tggcgttccg gtccaccatg agcagtcttc aggagcgccc
     1321 ggatgatgct tccaataggg ctgtcctgtt cgaccttcac gtggcgcgta gaccgcatgt
     1381 ccgcatagac cgcaaacgta acgcggtcga cgatccagcc gggctcttgt cgcaagccgc
     1441 ctgacaacct ggtaatcgtc tttgggcatc cccatcagag catcgcttga aatcgtaagc
     1501 atcgtctcca ggtcgatggc tctcttaagt gtcctcttga attgtttctc tgccatcgcg
     1561 acacaccatc cccatcgacc gcattctaac tatgtcgccc cagtgcaacc cggtggacca
     1621 acaacctttc tctgaggctt gctatggcag tgcgaacggt tccggacctg cttcgcctca
     1681 cgcaccaaag caaccgctca gacccaaggc ctctggctac tgagacgtcc cgtaaaccgt
     1741 cggacggttg tcaatgatat ccttgtcggc accgaccatg gttggccgaa tccaaagcaa
     1801 cctggtctca gtcagcgcct tcccaaatcg ttgatttcgc caatgtccac ggcgccagtg
     1861 accaatagcc gtcccgccga gcatcgctcg cctatcgaat gacgttcgat agccgcaaaa
     1921 attgatccaa cggcagccgt cctgacgtgc ccgcgcctcg gcaatctttc gagttttgcc
     1981 tttggcccgc ctaacctttt catgtgcccg cgggtcgttt agccgtttct cctgatcgcg
     2041 ttcggggtag ttcaaatagc agacgaggtt gaacaccagt cgcgtgacct cctccagaac
     2101 cgttggatcg gaaattacgt tgcggagatc atctggatgc ctgacatcct ctctgaaggt
     2161 ctccattgga agcgccgatc gtcggccgcg ttcgatgcca tcgg
//
LOCUS       DATMTI010000372         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_820906, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000372 DATMTI010000000
VERSION     DATMTI010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820906"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VN039_06135"
     CDS             complement(<1..203)
                     /locus_tag="VN039_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45585.1"
                     /translation="MVSTNIDQFDFIKIGIASPERVLNWSHGEVMKPETINYRTLKPE
                     RDGLFCERIFGPTKDWECYCGKY"
     gene            complement(216..>2354)
                     /locus_tag="VN039_06140"
     CDS             complement(216..>2354)
                     /locus_tag="VN039_06140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWV45586.1"
                     /translation="LTHKRRLSALGPGGLTRERAGFAVRDIHPSHYGRICPVETPEGP
                     NAGLIGSLSTYARVNEYGFLETPYWEVKDGVLTGEIHFLSADEEDKFRVAPGDAPRSS
                     EGVLLGSHVPVRYRNEFTISDPDMVDYVGVSPIQIVSVGTALIPFLEHDDANRALMGS
                     NMQRQSVPLVQAERPMVGTGLERRVAMDSGMSLAAEVDGTVERVTGENIEIRGKDGKI
                     VTHQMIKYLRSNQDTCLNHRPIVRPGDKVEIGQVIADGAATQLGELALGRNIMVAFMP
                     WEGYNFEDAILISERLIHADVYTSIHIEKLEVDARTTKLGPEEITREIPNVGEDALRN
                     LDERGIVRVGARVFAGDILVGKITPKGESEHPPEEKLLRAIFAEKARDVRDNSLRVPH
                     GEGGRVVDVKVFDREKGDELPPGANTVVRVYLAQKRKISVGDKMAGRHGNKGIVSKIL
                     PIEDMPFMPDGTPIDIVLNPLGVPSRMNVGQTYETLLGLAAHLTGLHYEVPPFDEMYG
                     EEASRNAVTKELQRAVDEAGIDWIKNDGKAILYDGRSGEPFGNRVAIGRMYMMKLVHL
                     VDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTIKSD
                     DVVGRSRAYEAIVKGENLNRPSIPESFKVLVREIHSIGLDITVKKRLKDGREVEIDLM
                     ADEAARAPKREFASAGNELLNFFSANSDSI"
BASE COUNT          473 a          681 c          673 g          527 t
ORIGIN      
        1 ttgtacttgc cgcagtagca ctcccagtcc ttcgtcggac cgaagatgcg ctcgcagaag
       61 aggccgtccc gctcgggttt gagggttcgg tagttgatgg tttcaggctt catgacctcg
      121 ccgtgagacc aattaaggac acgctcgggt gaggcaatcc cgattttgat gaaatcaaac
      181 tggtcaatat ttgtgctaac cacagtggga ctcctttaga ttgaatcgga attggctgag
      241 aagaagttga gcagctcgtt gccggcggag gcaaactcgc gcttgggagc gcgggcggct
      301 tcatcggcca tcaggtcgat ttcaacttcc cttccgtctt tcaaacgttt cttgaccgta
      361 atatccagac cgatactgtg gatctcgcga accagtacct tgaaggattc cgggatgctc
      421 gggcggttca ggttttcacc tttcacaatc gcctcgtaag cacgggaacg gccgaccacg
      481 tcgtcagact tgatggtgag catttcttgc agggtgtaag cggcgccata agcttccagc
      541 gcccagactt ccatctcccc gaagcgctga ccaccgaact gagccttacc acccaacggc
      601 tgctgggtaa ccaagctgta ggggccggtg ctccgagcgt ggatcttatc atccaccaag
      661 tgaaccagct tcatcatgta catacgaccg atggccaccc ggttaccgaa cggttcgcca
      721 ctgcgcccat cgtacagaat cgctttgccg tcatttttaa tccagtcgat accggcttcg
      781 tccacggcgc gttgcagttc tttggttact gcgttgcggg acgcttcttc accgtacatt
      841 tcatcgaacg gaggcacttc gtagtgcagg ccggtcaagt gggctgccag acccaacagg
      901 gtctcatagg tttggcccac gttcatccgg gaaggtacgc ccagcgggtt caacacgatg
      961 tcgatcggag tgccatccgg catgaatggc atatcttcga tgggcaggat tttggacacg
     1021 atccctttgt taccgtgacg tccagccatc ttgtcgccga cgctgatctt ccgcttttgg
     1081 gcgagataca cgcgaaccac cgtgtttgcg ccaggaggca gctcgtcgcc tttctcgcgg
     1141 tcgaacactt tcacgtcaac cacgcgaccg ccttcaccgt gaggaacgcg cagcgagttg
     1201 tcacgcacgt cgcgggcttt ttccgcgaag atggcgcgca acagcttctc ttccggcgga
     1261 tgctcggatt cgcctttggg cgtgatcttg ccgaccagga tatcgccagc gaatacacgg
     1321 gcgccaacgc ggacgatacc acgttcatcc aggttgcgca gggcgtcttc accaacgttg
     1381 gggatctcac gagtgatttc ttccgggccc agcttggtgg tacgggcatc cacttctagt
     1441 ttctcaatgt ggatactggt gtagacatcc gcgtggatca ggcgttcgct gatgaggata
     1501 gcatcctcaa agttgtagcc ttcccagggc atgaacgcga ccatgatgtt gcggcccagc
     1561 gccagttcac ccagttgggt agcggcgccg tcagcgatca cctggccgat ttccacttta
     1621 tcgccgggac ggacgatcgg acggtggttc aagcaagtgt cctggttgct gcgcaggtat
     1681 ttgatcatct ggtgggtgac gattttgccg tctttaccac ggatctcgat gttttcaccg
     1741 gttacgcgct caacggtacc atccacttcg gcagccaacg acataccgga gtccatggcg
     1801 acccggcgct ccaggcctgt tccgaccatc ggacgctcag cctgcaccag cggtacggac
     1861 tgacgttgca tgttggaacc catcagcgca cggttggcgt catcgtgctc caggaacgga
     1921 atcaacgctg tcccgacgga cacgatctga atgggggata caccaacgta gtcaaccata
     1981 tcgggatcgg agatggtaaa ttcgttgcgg taacgtaccg gaacgtggct gcccagcagg
     2041 acgccttcgg aggaacgcgg tgcatcgccg ggagccacac ggaacttatc ctcttcatca
     2101 gcggacaaga agtggatctc tccggtcaag acgccatctt taacttccca gtaaggggtt
     2161 tccaggaagc catattcgtt aacgcgggcg taggtactca aggagccaat caagccggcg
     2221 ttcgggcctt ccggggtttc taccgggcag atccgaccgt agtggctggg gtgaatgtcg
     2281 cgaaccgcaa agccagcgcg ctcacgggtc aagccgccag gcccgagggc tgacaggcgg
     2341 cgtttgtgcg tcaa
//
LOCUS       DATMTI010000373         1685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_523728, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000373 DATMTI010000000
VERSION     DATMTI010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523728"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /gene="uvrA"
                     /locus_tag="VN039_06145"
     CDS             <1..1348
                     /gene="uvrA"
                     /locus_tag="VN039_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWV45587.1"
                     /translation="GQRIRLATQIGSGLVGVLYILDEPSIGLHQRDNRRLLQTLLKLR
                     DLGNTVVVVEHDAETMQAADHLLDMGPGAGSHGGHVIAQGTPQQIMGDPNSLTGQYLR
                     GAQTVSVPQRQRKERERLSVIGAQKHNLKNVTARIPLGFFTCVTGVSGSGKSTLVLEV
                     LFHSLSQLLYHKKPKIDGCKELKGVDALDKVIDIDQSPIGRTPRSNPATYTGLFGYIR
                     DLYSNLPESRVRGYKPGRYSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCEVCKGQR
                     YNRETLEILHKGKSIADVLNMTVDDALEFFEHIPLIKAKLQTLHDVGLHYVKLGQSAT
                     TLSGGEAQRVKLSRELSKRATGRTMYILDEPTTGLHFADVQRLLDVLDRLVEAGNTVL
                     VIEHNLDVIKNADWIIDLGPEGGDRGGEIVAEGPPKDIAKVKRSYTGQVLREAGVG"
BASE COUNT          393 a          431 c          515 g          346 t
ORIGIN      
        1 ggggcaacgg attcgtttag cgacgcagat cgggtccggc ttggtcggcg tgttatacat
       61 cctggacgaa ccctcgatcg gacttcatca gcgcgacaat cgccggttgt tgcagacgtt
      121 gctgaaacta cgagatcttg gcaacacggt ggtggttgtc gagcatgatg ccgagacgat
      181 gcaggctgcc gatcatcttc tcgacatggg acccggcgcc ggctcgcatg gtgggcatgt
      241 gatcgcgcag gggacgcctc aacagatcat gggcgatccc aattcgctga cgggccaata
      301 cctgcgcggc gcccagactg tgtccgtgcc gcagcggcag cggaaggagc gggaaaggct
      361 gtcggtcatt ggagcgcaga agcacaattt gaaaaatgtg actgcgcgca ttcccctggg
      421 attcttcacc tgtgtgaccg gggtgtccgg atcaggcaag agcaccctcg tgctggaggt
      481 cctgttccac tctctttcgc agctgctcta ccacaagaaa ccgaagatcg atggatgtaa
      541 agaattgaaa ggcgtcgatg cgctcgacaa agtcatcgac attgatcaat cgccgatcgg
      601 ccgtacgcct cggtctaatc ccgccaccta tacgggcttg ttcggttaca tccgtgatct
      661 ctattccaat ctcccggaat cacgagttcg tggctataag ccggggcgtt atagcttcaa
      721 cgtaaaaggg gggcggtgcg aggcttgtca gggcgatgga ctcatcaaga tcgagatgca
      781 cttcctgccg gatgtctacg tgacctgcga ggtctgcaag ggacaacgct acaaccgcga
      841 aacactcgaa atcctccata aggggaagag catcgccgat gtgttgaata tgacggtgga
      901 cgacgcgttg gagtttttcg agcacattcc attgatcaag gcgaaacttc agaccctgca
      961 tgatgtcggc ctgcactatg tgaagctcgg tcaatcagcg acgacgctct ccggcggcga
     1021 agcgcaaaga gtaaagctgt cccgcgagct ctctaaacgc gcaaccggac gcaccatgta
     1081 tatcctcgat gaaccgacaa cgggtctgca tttcgccgat gtgcagcgat tgctggatgt
     1141 gttggatcgg ctggtcgaag ccggaaatac cgtgctggtg atcgaacaca atctggatgt
     1201 catcaagaac gcggattgga tcatcgattt ggggcctgaa ggtggagatc gaggtggcga
     1261 gattgtggcc gaaggaccgc cgaaggacat tgcgaaggtc aagcggtcgt acacggggca
     1321 agtgttaaga gaggcgggcg tcgggtgaag ggcgcggtca cggaaggatg agcattccgg
     1381 gttcgccata atgcgaccga acttcgacag caacttgtcc acttacccct tggctatgcg
     1441 ccttaccgga ctgactgaca gaacggcgcc cagcaccgtt actgccaatg gatggcaata
     1501 gcgctacggc gaagccttca tgctcatccc ttccgcccgc cttgaggaag gaaggggaag
     1561 tggtggcgaa tagcgctaac agctgtcagc tacatcagaa gtggacaaat gacgagtgtg
     1621 ggcgaagctg aaaagagatt tgaaaaatat cgtgataatt tgagaagaga ggttggtctt
     1681 ctcgc
//
LOCUS       DATMTI010000374         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_820985, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000374 DATMTI010000000
VERSION     DATMTI010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820985"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..764
                     /locus_tag="VN039_06150"
     CDS             351..764
                     /locus_tag="VN039_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45588.1"
                     /translation="MSAEAAQAEGFFDRMLDRTVESAERKARDRANQRIDQSIDKVIN
                     KTEETVQCVATDQECLKRAKDAGKQVSIVNQPTLSDSAKCVVTDTACLKQAKAQGKKV
                     EIVEEAELDTLRCSVADAECLKRAKTMGKKVEITD"
     gene            819..1070
                     /locus_tag="VN039_06155"
     CDS             819..1070
                     /locus_tag="VN039_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45589.1"
                     /translation="MTAIPKHSTVCQNRDSMGRNKKLRVRIASLGQRITDHQIKIAIE
                     RQRATPNVSLIRHWAVEITAWEQTVGQLTRRLKKGKRHD"
     gene            1063..1335
                     /locus_tag="VN039_06160"
     CDS             1063..1335
                     /locus_tag="VN039_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45590.1"
                     /translation="MIKKRGRPKISDTGTLLVPSSALLDNHFEELLEAIEEIRGLTAL
                     LKSISSRDEKREEYEGRLYAALTHLDHHVKPAIKEWDRVVDRMADA"
     gene            complement(1347..>2202)
                     /locus_tag="VN039_06165"
     CDS             complement(1347..>2202)
                     /locus_tag="VN039_06165"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HWV45591.1"
                     /translation="LPSKWSVKDLYLAHFAVTDITGKRFHFSEKFSREGLGKAGADES
                     RLRVWIDDWRAEASTEPSASHALVAHDETHALALTLQPAKPLVTHGAAGISRKGKDVG
                     QASHYYSFTRLETSGKLTIDGESFDVTGTSWMDHEFGSTELGADQVGWDWFSIQLEDD
                     TELMLYRMRRKDGSSDLASSGTAVSPDGGARHLEVTDFQIESTGTWTSPESKATYPSK
                     WRLKFPSLDLVLDVTPLVADQELRTSRSTKVSYWEGAVAVTGTKQGRPIKGQGYVELT
                     GYAERLKM"
BASE COUNT          516 a          619 c          603 g          464 t
ORIGIN      
        1 cagctttgtg aatcccttaa ctgacgacac gccctaaccc atcgagacca acgcatgcaa
       61 acggcgcggc tcaaaacgct agacaccccc tgatccatta tgagcatcta tgcgacactc
      121 tggagtctta agtttccgaa gtatagggac gcccacactg gatgcgaatg ggctgaggtg
      181 acggcacaag cagtgccacc gcatgttggc tcttcgacgc cgggatgctc caatcgggct
      241 ccattcttgg tctacccttt atgctgtgct agagtgctgc tgtgcatatt cctaaggagg
      301 gcattgcgat gaagacacac tggatagtcg gtctctcggt tgtcatagta ttgagcgccg
      361 aagcagctca ggccgaaggt ttctttgacc gcatgcttga ccgcacggtg gaaagcgccg
      421 aacgtaaggc tcgcgaccgg gcgaaccagc gcatcgatca atccatcgac aaggtgatca
      481 acaaaactga agagaccgtg cagtgcgtcg ccaccgatca ggagtgtctg aagcgggcca
      541 aggacgcagg caagcaggtc tcgattgtca accaacctac cctatctgat tcagcgaagt
      601 gcgtcgtgac ggatactgct tgcctcaaac aggccaaggc gcaagggaag aaggttgaga
      661 tcgttgaaga agctgaactc gacacgctgc gttgctcggt ggccgatgcc gaatgcctta
      721 aacgtgcaaa aaccatggga aagaaagtcg aaatcaccga ctgacgggtt cgatagtcct
      781 cgtcctcact cccttccctc ctacttacac ggagccagtt gacagctata ccgaagcaca
      841 gtacggtttg tcagaatcgg gacagtatgg ggcgcaataa gaagctgcga gttcggattg
      901 cgagcttagg acaacgaatc acagaccatc agattaagat cgcgattgaa cgtcagcgcg
      961 cgactccgaa cgtgagcctc attcggcatt gggcagtcga gattacggcg tgggaacaga
     1021 ccgtcggcca attgacccgc agattgaaga aaggcaaacg ccatgattaa gaagagaggg
     1081 cggccgaaaa tatccgacac agggactctg cttgtgccgt cgagtgcgct cttggacaat
     1141 cactttgagg agctgcttga ggcgatcgaa gagattcgtg ggctcaccgc gctcttgaaa
     1201 tccatttctt cgagagacga gaaacgagag gaatatgaag gccggttgta tgcagccctc
     1261 acccatcttg accatcacgt taaaccagcc atcaaggaat gggatcgcgt ggtggatcga
     1321 atggcagacg cctaatccat tctcccttac attttgagac gctcggcata gccggtcagc
     1381 tcgacatagc cctggccttt gataggccgg ccttgtttgg tgcctgtcac ggccaccgct
     1441 ccttcccaat aggaaacctt ggtgctgcgc gaggtgcgca attcctgatc cgccaccaac
     1501 ggagtgacgt ccagcaccaa atcgagcgac gggaacttca gccgccattt gctggggtac
     1561 gtggccttgc tttcggggct ggtccaggtt ccggtcgatt cgatttgaaa gtccgtcacc
     1621 tccaggtgtc gagcgcctcc atccggggac acggcagttc cgctcgacgc aagatcggac
     1681 gatccatctt tccgacgcat ccgatacagc atgagttcgg tatcgtcctc taactggata
     1741 ctgaaccaat cccatccgac ttgatccgct cctagttcgg tcgatccgaa ttcatggtcc
     1801 atccagctgg tgccggtcac atcgaatgac tcaccgtcga tcgtgagctt cccgctcgtc
     1861 tcaagcctcg tgaacgaata gtagtgagag gcttgaccca catctttccc ttttcgactg
     1921 atgccggcag cgccatgagt aaccagaggc ttggccggtt ggagtgtgag ggcaagggca
     1981 tgcgtctcgt cgtgggctac caaagcgtgt gaagccgatg gttccgtcga tgcttcggcc
     2041 cgccaatcgt cgatccagac acggagccgc gactcatcgg ccccggcctt tcccaacccc
     2101 tcacggctga acttttccga gaaatgaaat cgcttcccgg tgatgtcggt cacagcgaaa
     2161 tgagccagat acagatcctt caccgaccat tttgacggga gt
//
LOCUS       DATMTI010000375         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_608218, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000375 DATMTI010000000
VERSION     DATMTI010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608218"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VN039_06170"
     CDS             <1..281
                     /locus_tag="VN039_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45592.1"
                     /translation="KKLVASLLSIFVLLLGIFLSLGSSDSPSGLKIHTTQWIILNYAC
                     LLVWLFVWMIDQARVRGKNVWLWLLPFVLAPLPTLMLFVLFLQRRLSS"
     gene            complement(276..1568)
                     /locus_tag="VN039_06175"
     CDS             complement(276..1568)
                     /locus_tag="VN039_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV45593.1"
                     /translation="MKQGAFDLTGLMNRRILVMLPLGFASGLPLALTSGTLQAWLTVE
                     GVDLKTIGIFTMVGLPYTLKFLWAPVMDRVIPPWLGRRRGWMVLTLLCVAIGLVLMAA
                     TNPRVHPGWMAAYALLVAFLAASLDIVFDAYRTETLRSHERGLGAAVWVNGYRIALLA
                     SGAGALVLADHVGWQMTYLAMAAVMLAGLFMVLISPDPTFAAEAPKSMREAVGAPLAE
                     FFGRPHALGFLAVIILYKLGDAFASTLQTAFLIGGLGFSATDVGAVKGLGVFATLFGA
                     FFGGLLMTKSGLVRSLLIFGVLQAVSNLGFVALAVAGKNATALTAVIVVENVTGGMGT
                     AAFVALVMSLCDPRYTATQFALLSSLEALGRVFAGRPSADVVTLVGWTQFFVLTVVVA
                     LPGLWAVWRVRRFLEPEQRTVVRTSLSEPPVTPVSERL"
     gene            complement(1715..2059)
                     /locus_tag="VN039_06180"
     CDS             complement(1715..2059)
                     /locus_tag="VN039_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45594.1"
                     /translation="MGWSTDSLPAEPDLNTRVDELYDHESRLFIMLYSLSGNGKVDYV
                     TARLVQEHSRSTYGNPIYQTEAQPLFYWWNHIMWNDPEQDGVNGNEKIYQENTDFDIS
                     RYKPCLFNGQPC"
     gene            2259..>3154
                     /locus_tag="VN039_06185"
     CDS             2259..>3154
                     /locus_tag="VN039_06185"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HWV45595.1"
                     /translation="MVTTRASLHTLGCRLNQAETSVLGEGLRRKGFELVEFGQPTDLL
                     VLNTCAVTEDAERTSRYLIRKTLKHSPHAFIAVTGCYAQTGMDRLNQQTGIDLIVGHQ
                     FKLDLPAYLPPANELRKRSAIEIRHTKTIMRGDFELPHFAESDSTRALLKVQDGCSAM
                     CSFCIIPFARGHERSRTFEDIQREVEALAARGYREVVLTGVNIGQYAYQGRDFYSLLR
                     WLDQAADFERIRISSIEPTTVGEELLDLIASSKKLCPYLHIPLQSGDDQILQAMNRRH
                     TIKSYIKLIEKALITIPRLGIGT"
BASE COUNT          789 a          922 c          811 g          632 t
ORIGIN      
        1 gcaagaaact cgtcgccagc ctattgagca tattcgttct attgttggga atcttccttt
       61 ccctaggttc ttccgacagt ccgagcggtc tgaagattca cacgacccag tggatcatat
      121 tgaattatgc atgtctatta gtctggcttt ttgtctggat gatcgaccaa gcgcgagtcc
      181 gcggcaagaa tgtctggtta tggttgttgc cgtttgtgct ggctccatta cctaccttga
      241 tgctcttcgt gctcttcctt caacggcgac tgtcctcata gccgctcgga caccggtgtg
      301 accggaggct ctgagagaga ggtgcgaacg accgtcctct gttctggttc gagaaagcgc
      361 cggacccgcc aaacagccca aagaccaggc aaagccacca ccacggtcaa gacgaaaaat
      421 tgtgtccatc cgaccagggt cacaacatct gccgatgggc gaccggcaaa gacccttcct
      481 aacgcctcta atgaagacag cagcgcgaac tgagtggcgg tataccgagg atcgcagagc
      541 gacatcacca acgctacgaa cgcagcggtg cccattcctc ccgtcacatt ttcaaccacg
      601 atcaccgccg taagggcagt tgcattcttg ccggctacgg ccaatgcgac aaatcccagg
      661 tttgagaccg cttgcaagac accgaagatc aatagggagc gcactagtcc ggacttggtc
      721 atcaaaagtc caccaaaaaa tgcgccgaac aatgttgcaa aaacccctaa ccccttcaca
      781 gcacccacat ccgtggcgga aaagccgagt cctccgatca gaaaggccgt ctgaagcgtt
      841 gacgcaaagg cgtctcccag tttatagaga atgatcacgg ccaagaagcc caacgcgtga
      901 ggccgtccaa agaactccgc cagtggtgca ccgactgctt ctctcatact cttcggagct
      961 tcggcggcga atgtagggtc cgggctgatt aggaccatga acaaccccgc cagcataacg
     1021 gctgccatcg ccagatacgt catctgccat cccacatgat cggcaaggac caacgcgccg
     1081 gctccggagg ccaaaagagc gattcgatat ccgttcaccc acacggcggc acccaatccg
     1141 cgctcgtgag aacggagcgt ttccgttcga taggcatcaa aaacaatatc caaagaagcg
     1201 gcaagaaacg cgacgagcag ggcataagcc gccatccatc caggatgaac ccgcggattg
     1261 gtcgccgcca taagcaccaa cccgatcgca acacagagta gtgtcaggac catccagccg
     1321 cgccgccggc ctaaccaggg agggatcaca cgatccatca cgggagccca aaggaacttg
     1381 agggtatagg gaaggccgac catcgtgaaa atgccgatgg tcttgagatc gactccttcg
     1441 accgtaagcc aggcctgtaa ggtcccggac gtgagcgcaa gggggagacc ggacgcaaaa
     1501 cccaacggca gcatcacgag gatgcgtcga ttcatgagac cggtaagatc gaaggctcct
     1561 tgtttcacag agactctcgt acgagcgtga gccgaatcag tcagacctgc atactggagc
     1621 gattttccgc cgcaaggcag aataccatcc cctcccaggg ctgttcagca gaatcacaaa
     1681 acatgcggga acggcctcac cggccggtgt agaactaaca gggctggccg ttgaacagac
     1741 atggcttgta tcgcgagata tcgaagtcgg tattttcctg atagatcttt tcgttgccgt
     1801 tgaccccgtc ctgctccgga tcgttccaca tgatgtggtt ccaccaatag aacagcggtt
     1861 gggcttccgt ctggtagatg ggattaccat aagtactgcg agaatgctcc tgaaccagcc
     1921 tggccgtcac atagtcgact ttgccgtttc cgctaaggga atacagcatg ataaagagac
     1981 gggattcatg atcatacagc tcatcgacgc gagtattgag atcgggctcg gcaggaagcg
     2041 aatccgtgct ccatcccatc acaggatggc tccagagcaa ccctgtaaaa acaaaaacaa
     2101 tgagagcacg cagatgcacg atctttacgc tctccccgac gactcggatg tgatcccagc
     2161 atgggtcttt ccggcaatta gccgacgttc accgcaacct ctcggccgca acaggcaacc
     2221 ttgccggaac aggcatgatc ttcctataat gccaccctat ggtgacgacc cgtgcatctc
     2281 ttcatacgct cggctgccga ctgaatcaag ccgaaacctc ggtccttggg gaggggctca
     2341 ggcgaaaggg gtttgagctc gtcgagttcg gccaaccgac ggatctcctt gtgctcaata
     2401 cctgtgcggt tacggaagac gccgaacgga catcgcgata tctgatccgg aagaccttaa
     2461 aacactctcc ccatgctttt atcgcggtga cagggtgcta tgcccaaacg ggaatggacc
     2521 gcctcaacca gcaaacgggg atcgatctca tcgtgggcca ccaattcaag ctcgatctgc
     2581 cggcttatct tcctcccgcc aacgagttac ggaaacgatc ggccatagag attcgtcata
     2641 ccaaaacgat catgcgcgga gacttcgagc tgccgcattt cgcggaatcg gactccaccc
     2701 gtgcattgct caaagtacag gacggctgca gcgcgatgtg cagtttctgc attattccgt
     2761 ttgcacgagg tcatgagcga agcagaactt ttgaggacat tcaacgtgaa gttgaagcct
     2821 tagccgctcg aggatatcgg gaagtcgtac tgaccggagt gaacatcggc cagtatgcgt
     2881 atcagggccg agacttttat tcgttgcttc gttggctcga ccaagcggcc gacttcgaac
     2941 gcatccgcat ctcttcaatc gaacccacaa cggtcggcga agaactgctt gatctgatcg
     3001 cctcgtcgaa gaaactctgt ccctatcttc acatcccctt gcaaagcggg gacgatcaga
     3061 ttttgcaggc catgaaccgc cgtcatacga tcaagagcta tatcaaactg atcgaaaagg
     3121 ctctcataac catcccccgc ctcggcattg gaac
//
LOCUS       DATMTI010000376         1568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_56529, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000376 DATMTI010000000
VERSION     DATMTI010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56529"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VN039_06190"
     CDS             complement(<1..301)
                     /locus_tag="VN039_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45596.1"
                     /translation="MRSFLPLCSFRLREGILVVAAMVSALLSGCGVGYTIVKSEAKLD
                     RLSVPMTKAQVLQEIGRPDRIIRDDGRMLVWEYSLTSRKQWLYEIAYCPISILFGG"
     gene            complement(381..941)
                     /locus_tag="VN039_06195"
     CDS             complement(381..941)
                     /locus_tag="VN039_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HWV45597.1"
                     /translation="MSTELRKGIFLIAAPSLRDPNFRQTVVLLCEHGSEGALGVIVNR
                     PTAVSISEALPQVPVIEGSGHVLYAGGPVQTNQVMLLYRGDQFPDNAHHVFDGVCLGG
                     DVGMVERILTGVGTKESFRAYLGYSGWGPGQLENEMKTGSWITLPADSQAVFEKDPTR
                     VWGDILFTLGEAYQPYADMPFDPSCN"
     gene            complement(1015..>1568)
                     /locus_tag="VN039_06200"
     CDS             complement(1015..>1568)
                     /locus_tag="VN039_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWV45598.1"
                     /translation="AGLPEESGGCCYNSAVVVGPSGFLGCYRKTHLFYEETQCFTPGD
                     SGFLVWNIGSARIGIMICFDWYYPEAARTLAVQGADIICHPSNLILPNCPDSMPVRCL
                     ENRVFAVTCNRIGSEARDGKERLTYIGQSEVVTPKGIIQHRASCDQEELTVVEIDPAQ
                     ARNKSLNRYNDLLRDRRTSLYKM"
BASE COUNT          381 a          465 c          403 g          319 t
ORIGIN      
        1 acccgccgaa caatatcgaa atcgggcaat aggcgatctc gtacagccat tgctttcttg
       61 acgtcagaga atattcccag accagcatcc gtccatcatc acgaataatt cggtccggtc
      121 tccctatttc ctggaggacc tgcgctttgg tcatgggaac cgacaatcga tccagttttg
      181 cctctgattt gacgatcgta taaccgacgc cacagcctga taagagtgcc gacaccatag
      241 ctgcgacgac caatatgcct tcacggagac ggaaagagca gaggggaagg aaggatcgca
      301 tcgggcatga accctaccat cctgcgaggc tagcttcaac atgacatggt gggttctgcc
      361 gcagaaacgg cccggagcgt tcagttacag gatggatcga agggcatgtc ggcgtatggt
      421 tgataggctt cacccagcgt gaaaaggata tccccccaga cacgcgtcgg atctttttca
      481 aacaccgcct gcgaatccgc gggcaaggtg atccacgagc cggtcttcat ctcattttcc
      541 agttgtccag gcccccatcc tgaataccca agataggccc gaaaagattc tttcgtcccc
      601 acgccggtga gaatgcgttc gaccataccg acgtccccgc caagacagac gccgtcgaac
      661 acgtggtgcg cattatcggg aaactgatcg ccacgataga gcaacatcac ttgattggtc
      721 tgcacaggac caccggcata gagcacatga cctgatccct cgatcaccgg aacctggggt
      781 agggcttcag aaatggacac ggccgtcgga cgattcacga tgacgccgag cgctccttcc
      841 gacccatgtt cgcacaacaa cacgacggtc tggcgaaagt tcgggtcacg caggctcggc
      901 gcagcgatca aaaaaatgcc tttgcggagt tcagtgctca tgaacaaaat cctaccctgc
      961 ccacgcaact tgcgcaagca ctgggcttct ctcgacagga tatgaaactt gggattacat
     1021 cttgtacaac gacgtgcggc ggtcgcgaag cagatcattg tagcgattca agcttttgtt
     1081 gcgcgcttgc gccggatcaa tctcaacgac ggtcagctct tcttggtcac aagacgcccg
     1141 atgttgaatg atccctttgg gtgtcaccac ttcgctctgg ccgatatagg tcagccgctc
     1201 tttgccatcc cgcgcttcgc tgccgatacg attgcacgtc acggcgaaca cccgattttc
     1261 aagacaccgg accggcatgg aatccgggca gttcggcaag atcaagttgg atggatggca
     1321 aatgatgtct gccccttgta ccgctaaggt ccgcgccgcc tccggatagt accaatcgaa
     1381 acaaatcatg atgccgatcc tcgccgaccc gatattccac acaaggaacc ctgagtcacc
     1441 gggagtgaag cattgggtct cttcgtaaaa gagatgggtc ttccgatagc acccaagaaa
     1501 acccgacggc cccacgacca cggcggaatt gtaacagcag cccccggact cttcggggag
     1561 tcctgcca
//
LOCUS       DATMTI010000377         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_821252, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000377 DATMTI010000000
VERSION     DATMTI010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821252"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VN039_06205"
     CDS             <1..180
                     /locus_tag="VN039_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45599.1"
                     /translation="ITLQASELRPQNVLSSIAGAQGGNGSGDPLSKGGEGGKITIINI
                     NIEPSAIVNSSPGHP"
     gene            581..1687
                     /locus_tag="VN039_06210"
     CDS             581..1687
                     /locus_tag="VN039_06210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV45600.1"
                     /translation="MTTLSLSMIVKNEEALIERVLACAKTFCDEMIVVDTGSTDRTVE
                     LAEKMGAKVYHFEWIEDFAAARNYSLAQCTGDWVMWLDADDIITPECQQKFKEIKETV
                     LNDSLNGVVVPYYYLFDAEDNCTCINRRERMFRRAAGFQWEHPIHEVIYLTGETITRE
                     DVWIEHRFLPGKDSPKRNIRVLAKAMQKEEHYNNQRMVYIAAKELRLQNMFDKSIEYF
                     ERFLELNPSRRGWQAYDVYMCMGLCYKRMDNRLDDARRCFTSAMHADSRRAEAYDELA
                     FLHYEAGEWQYAIPYFETALRCVPPLGEDLIALEHYGWLPNEYLSRCYANVGNYYKAI
                     EVGMKGLPEHPNKANMLATITWYVSELSRQLGIE"
BASE COUNT          417 a          454 c          512 g          428 t
ORIGIN      
        1 atcacgcttc aagcgtcaga gctgcggcct caaaatgtcc tctcttctat tgctggggca
       61 caaggcggca atggtagtgg tgatccgctt tccaaagggg gtgaaggcgg taaaatcacg
      121 attatcaata tcaatatcga gcccagcgct atcgttaact catccccggg gcatccctaa
      181 cagtggtagt ctgcctgatt cggggcatta tttcttcgat gctgactccg tttcaggttg
      241 agaaaaactg atgttttaat gcagaacaaa cggcttctca ccgatgaggt aagaggccgt
      301 ttgcctggca aatttattaa ttccccaatg gggataggga agtttaatca tacgatttat
      361 cccccattgg gaggactgat tcgggaatcc aggagggcta tactgatcgt ggtctcctgt
      421 ggctatccgt tcgggtgggc gtgtgtgact gtttctcgca gtgagccggt tgattggtaa
      481 agaccagccc gtatctcccc tgaggcaaac ccaagctgct tccatgcgaa ttcattgtgg
      541 ctttcccatg gactttggct gattttatgg agtgaagact ttgacgacgc tatctctaag
      601 catgattgtg aagaatgagg aagccctcat tgagcgggtg ctggcctgcg cgaaaacctt
      661 ttgcgatgaa atgatcgtgg tggatacggg ttctaccgat cgcaccgtgg aactggccga
      721 gaaaatgggc gctaaagtgt atcactttga gtggatcgag gattttgcag cggcccggaa
      781 ttactcgctg gcccaatgta ccggcgattg ggtcatgtgg ctggatgccg atgacatcat
      841 cacgccggag tgccagcaga agtttaaaga gatcaaggag accgtgctga acgactccct
      901 gaacggcgtg gttgtgcctt actattacct gttcgatgcg gaagacaact gcacctgcat
      961 caaccgccgg gagcgcatgt tccgtcgtgc ggcgggcttc cagtgggagc acccgatcca
     1021 cgaggtcatc tatctcaccg gtgagacgat cacgcgggaa gacgtgtgga tcgagcaccg
     1081 cttcctgccg gggaaagata gccccaagcg caacatccgt gtgctggcca aggccatgca
     1141 gaaggaagag cactacaaca accagcgcat ggtctacatc gccgccaaag aattacgctt
     1201 gcagaacatg ttcgataaat ccatcgaata cttcgagcgc ttcctggagt tgaatccctc
     1261 ccggcgcggc tggcaggcgt atgacgtgta catgtgcatg ggcctctgct ataagcgcat
     1321 ggataacagg ttggacgatg cgcgccgttg ctttacgagc gccatgcacg ccgacagtcg
     1381 tcgcgcggaa gcctacgatg aactggcctt cctgcattat gaagccggtg agtggcagta
     1441 tgcgattccc tactttgaga cggccctgcg ttgcgtaccg ccgctgggtg aagacttgat
     1501 cgcgctggag cattatggct ggctgccgaa tgagtacctc agccgttgct acgcgaatgt
     1561 gggcaattac tacaaggcca ttgaagtggg catgaaaggc ttgccggagc atcccaacaa
     1621 ggccaacatg ctggcgacga ttacctggta tgtgagcgag ctatcccgtc agctgggcat
     1681 tgaataaggc caattcaaag aaaaagtttg agcgtataag tcttatggat aaaaagcccc
     1741 tgctatcact agcaggggct ttttgcgatt cgctgttcct gtctcgctac acgccgaccg
     1801 gcactttttc c
//
LOCUS       DATMTI010000378         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_608405, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000378 DATMTI010000000
VERSION     DATMTI010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608405"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..478
                     /locus_tag="VN039_06215"
     CDS             95..478
                     /locus_tag="VN039_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV45601.1"
                     /translation="MVTVGNLMQKEPVTVDAGTSVIEAAKLMKACNVESVLVTRQARI
                     IGILTESDVVKKFVGAEKVSYFVPVEDIMSSPLPGIEERRPLTEAADLMDKHRTLHLG
                     VTKGGTFIGLVSVRDFLRPVSIDDF"
     gene            623..2107
                     /locus_tag="VN039_06220"
     CDS             623..2107
                     /locus_tag="VN039_06220"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HWV45602.1"
                     /translation="MTRLDTHLAPHSRFSAISSQPERELKLAVAPNFHLPRLPGTPLP
                     RRQLISTYYDTASYDLAHARITLRHRIERGKKSWQLKIPLGIDRQEIEVTDEDAGPPA
                     SVRRLLTLHLGHRKLLPVVTLCVWRRGFLVRHGRVPIAEIALDSVSVDKNGRTTRCFR
                     ELEIEQRQGDEASLRSLEQRMREAGASDHDGRPKLFRALSLSAPSQPMQPEAKAPTVE
                     YIKWALAQHVGWLLAHDPGTRLGTDRESLHQMRVATRRLRAVLRTARPILLPAWLASL
                     QQELKWLSELLGPARDLDVQIAYFTEESSALNARDRKLLTAFISHLRTQREAVQQMVL
                     SELTSARYFELIRRLQQAAQAPSVVESSFTVRRLAKRAFKKLRKAINRLAPSPSDTQL
                     HKVRIKAKRARYAAELARRSVGKPAGRFIKSARVVQDLLGIHQDAIQAERYIRQFLKY
                     STSLRAGFVAGRMVERQRQRRHQVRKDMKPLFKALLKRGKQAWG"
BASE COUNT          606 a          845 c          735 g          532 t
ORIGIN      
        1 acattttact ggcccgtaat atctacctaa ccccgtcgca acagcacatt ggtagcctgg
       61 agccaataca aacataataa aaaggaggat cagtatggtt acggtcggca atttaatgca
      121 gaaagaacca gtgacggtgg atgcgggcac ctcggtcatc gaggcggcga aattgatgaa
      181 agcctgcaac gtcgaaagtg tgttggtgac ccgtcaggcg aggatcatcg gcatcctgac
      241 ggaatcggac gtcgtcaaaa agtttgtggg ggccgagaag gtgtcctact tcgtgccggt
      301 ggaggacatc atgagcagtc cgctgcccgg catcgaagag cgccggccgt tgacggaagc
      361 cgccgacctc atggacaagc accgcacgct ccacctcggc gtgaccaaag gagggacatt
      421 catcggcctg gtgtcggtgc gggactttct ccgaccggtg tccatcgacg acttttaacg
      481 accctacctt tcttgacccc gctgcctact tggctaggca gcctcggcga ccttccctgg
      541 tattccccga cgcccctgag ttgcatttcc tctctcctgc gttgacactg aagggagccc
      601 ggctctgccc gagggttcca cgatgactcg gttggacaca catctcgctc ctcatagccg
      661 cttctccgca atctcatcgc aaccggaacg cgaactgaaa ctagccgttg ctccaaattt
      721 ccacctgcct cggcttcccg gcacgccgct tccccgacgg caactgatct ccacttatta
      781 cgacaccgcc tcctacgact tggctcatgc gaggattacc cttcgtcatc gcatcgaacg
      841 ggggaagaag tcctggcagc tgaaaatccc attgggcatt gatcgacagg agatagaagt
      901 gaccgacgag gatgccggtc cgccagcctc ggtgcgtcgt ctactgactc tccatctggg
      961 acatcggaaa ctgttgccgg tcgtgacgct gtgcgtctgg cgaagaggtt tcctggtgcg
     1021 tcacggtcga gtacccatcg ctgagatcgc cctcgactcg gtgtcagtcg acaagaacgg
     1081 ccgtaccacc cggtgctttc gcgaactcga aatcgaacaa cggcagggcg acgaggcctc
     1141 actccgttcc ctcgaacagc ggatgcgaga agcgggtgca tccgatcacg acggacgacc
     1201 caagttattc cgtgccctct cgctctccgc cccttctcaa cctatgcagc ctgaggcgaa
     1261 ggcgccgacg gtcgaatata taaagtgggc cctcgctcag cacgtcgggt ggctcctcgc
     1321 ccatgatccc ggcacgaggc tcggaaccga tcgtgagagc ctgcatcaaa tgcgtgtcgc
     1381 gacgagacgc ctgcgcgccg tgctccgcac cgcgcgaccg attctcctcc ccgcgtggct
     1441 cgcgtcactg caacaggagc tcaagtggct cagcgagctg ctgggaccgg cccgcgacct
     1501 cgacgtccag atcgcttatt tcacggagga atcttccgca ttgaacgcac gagatcgcaa
     1561 gctgttgaca gcattcatct cccatctgcg cactcaacga gaggccgttc agcagatggt
     1621 gctcagcgaa ctcacgagtg ctcgatactt cgaactcatc cggcggcttc aacaagcggc
     1681 gcaggccccc tcggtcgtgg agtcctcctt caccgtccgc cggcttgcca aacgagcatt
     1741 caagaaactg cgcaaggcga tcaaccggct cgccccttct ccatcggaca ctcaattgca
     1801 caaggtccgc atcaaggcca aacgcgcccg ctatgcggcg gaactcgctc gtcgctcggt
     1861 ggggaaaccc gccggccggt ttatcaagtc cgcgcgtgtc gtccaggatc tactgggcat
     1921 ccatcaggat gcgatccaag cggaacgtta catcagacag ttcctcaaat actcgacgag
     1981 cctccgagct ggatttgtcg ccggtcgcat ggtggaacgc cagcgccaac gacgccacca
     2041 agtccgcaaa gacatgaagc cgctgttcaa ggctttgctg aaacggggga agcaggcgtg
     2101 gggatgaaaa tggtcaatgc ccccatcaca attatcggtg aacgatccag gacaagccta
     2161 caatagcccc acaacttcac caagctgagg ttcatgcctg acacacacca ccaccgcatc
     2221 caacgggagt gtacggagca atccaaccag ctgctcaggc ttcgctccga ccgccgattc
     2281 ttcccgtgtc tcgaccttga gagtcgaaca gatgaccgtg cgtagcagcc tggccgtatc
     2341 atagactcta ccgcgatgcc atggtgaacc agaatgcagt gcataaccac tcctccacat
     2401 agcagggcac aagcctaccg cagagggaca atgcagttaa cacaggtttt acctgttcgt
     2461 gaccgctctg gaacgatggt ccgatatcct cctgtgtagc tggaacggaa aagaaaggat
     2521 gccatgtcag cactcgaagc ggctcgtctg tagttcgcgt cacggctgtt cgatggagtc
     2581 cggcactgag aacagaacct gacgatgctg tgcgttttga cctataacaa acaagaggaa
     2641 ggtgctcaat gacccaaccg cttgcgacta aattattggc gcttgttcaa gagcggcgat
     2701 gtattacgct tgaagaac
//
LOCUS       DATMTI010000379         1977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_438599, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000379 DATMTI010000000
VERSION     DATMTI010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438599"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VN039_06225"
     CDS             <1..508
                     /locus_tag="VN039_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45603.1"
                     /translation="LRTEAEYHRAVAMLDEIIDEIGEQEIHPLADLVEILALTVSAYE
                     EIHVPVPVSSGPEILCALMEDHGLSPSDLPEVGSQGVVSEVFSGKRDLNVRQSFGSPL
                     DSGSRLRCSCRYRPARITRHEESEFAYREDDKTWLLLPAAGSIIVVPDALASVGGGEH
                     VDTPQGLC"
     gene            567..857
                     /locus_tag="VN039_06230"
     CDS             567..857
                     /locus_tag="VN039_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45604.1"
                     /translation="MATIQIKTPSPDQTIPLVKDALAMETKLTRDSLRTTDDRITVLT
                     RQLDVTPEQVLAGSVRRTEDNEALLLDLEGELELRRVLQDTLHHLEQLEVCP"
     gene            848..1237
                     /locus_tag="VN039_06235"
     CDS             848..1237
                     /locus_tag="VN039_06235"
                     /inference="COORDINATES: protein motif:HMM:NF039803.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HWV45605.1"
                     /translation="MSVADYVADIRRHILDTPHIVSHSLGYEERPPLGAIVNGSVTFA
                     DGSRVHFKEFLQFRPVTARLKYAYHYISSTHALIFRYDNARDPAARHLSTYPDHLHTP
                     DGLFPSFSPTFASVLREAATHVRRSSR"
     gene            complement(1322..>1977)
                     /locus_tag="VN039_06240"
     CDS             complement(1322..>1977)
                     /locus_tag="VN039_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWV45606.1"
                     /translation="AIVLRMPRTNYYEVLGVSREASDEDIKKAYRKLAFEHHPDRNPH
                     RKDADERIREINIAYEIVGDPEKRRSYDRLSWGDEPRAERVDPGIILDEMERKLFDEG
                     RKELFAILMKDVKRVKAELALVREQVVAEQGYDSFLEHVVIARASEIIGDFVTEEMDG
                     RKQRLVEVAVEMMVSQGVTKRGDEGGIRALRNRLETAFRNGRVHGIASALELFYERR"
BASE COUNT          401 a          646 c          450 g          480 t
ORIGIN      
        1 gttgcggaca gaggccgaat atcaccgagc ggtcgctatg ctcgatgaga tcatcgacga
       61 aatcggcgaa caagagattc atcctctggc tgatctggtt gaaatcttgg cgcttaccgt
      121 ctccgcatac gaagagattc atgtgccggt accggtctcc tctggtcccg aaatcctctg
      181 cgcactcatg gaggaccatg gattgtctcc atcggatctt cccgaggtcg gcagtcaggg
      241 tgtcgtctcg gaagttttct ccggcaagcg cgacctgaat gtccgtcaat cgttcggctc
      301 gccgctagat tctgggtctc gcctgcggtg ttcttgcaga taccgccccg cgcgcatcac
      361 tcgtcacgaa gaaagcgagt tcgcctatcg agaggacgat aagacatggc tccttttgcc
      421 tgcagcgggc agcatcatcg tcgtgccgga tgctcttgca tctgtcggcg gcggagaaca
      481 tgttgacact ccgcaaggcc tctgctagcg tcagtacaat tcgcatttga tcgatcgaat
      541 ggctattccg atgaaggaat cctcgcatgg ccacgattca aattaaaact ccctctcccg
      601 atcagaccat tccgttggtc aaagacgccc tggcgatgga aaccaaactt acgcgcgaca
      661 gcctgcgcac caccgacgat cgcatcaccg tattgacccg acaactcgat gtcactccgg
      721 agcaagtcct cgcaggctct gtccgtcgga cagaggacaa cgaggcgctt cttctcgatc
      781 ttgaaggcga gctggagctc cgtcgggtgc tgcaggacac ccttcaccat ctcgaacaac
      841 tcgaagtatg tccgtagccg actacgttgc ggacattcgc cgccatatcc tcgacacccc
      901 gcacattgtc tctcactcgc tcggatatga agagcgccct ccgctcggcg caattgtgaa
      961 tggctccgtt accttcgcgg acggatcgcg cgtccacttc aaagaatttc ttcagtttcg
     1021 accggtcacc gccagactca agtacgcgta ccactacatc tcttcgactc acgccctgat
     1081 cttccgatac gacaatgccc gcgaccccgc ggctcggcac ctctccacat accccgacca
     1141 tcttcatact cccgacggtc tctttccatc gttcagccct acgttcgcct ctgtcctgcg
     1201 cgaggcggcc acacacgtaa ggcgttcttc tcgatagttc gatcgcccac caccagctct
     1261 acgatttttc cttttactct cacctcatac cgtttcacgt ttcgcctttt acttttcacg
     1321 cctatcgccg ttcataaaac aattccagcg ccgacgcgat gccgtgcacg cgaccgttgc
     1381 gaaaggccgt ttcaagacga ttccgcagtg ctcgaatccc gccctcatcg ccgcgtttcg
     1441 tcaccccctg cgacaccatc atctccaccg ccacttcaac cagacgttgt tttcgtccgt
     1501 ccatttcttc tgtcacaaag tcacctataa tttctgacgc tcgcgcgata accacatgct
     1561 ccagaaatga atcatagccc tgctctgcca caacctgttc tcggacgaga gctagttccg
     1621 ccttcacccg cttcacatct ttcatcaaga tcgcgaacaa ttcttttctg ccttcatcga
     1681 agagcttccg ctccatttca tcgaggatga tgccgggatc gaccctttca gcacgcggct
     1741 catctcccca agacaaccgg tcgtagcttc tccgtttttc agggtctccg acgatctcat
     1801 acgcgatatt gatctctctg atccgctcgt ccgcatcctt tctgtgagga ttccggtcgg
     1861 gatggtgctc aaaggccagc tttcgatagg ccttcttaat atcttcgtcg gacgcctcac
     1921 gtgagacgcc aagtacctcg tagtaattcg tccgtggcat gcgaaggact atagcat
//
LOCUS       DATMTI010000380         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_56609, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000380 DATMTI010000000
VERSION     DATMTI010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56609"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1552)
                     /locus_tag="VN039_06245"
     rRNA            complement(<1..>1552)
                     /locus_tag="VN039_06245"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          295 a          488 c          364 g          405 t
ORIGIN      
        1 ggcacccctt ctcccgaagt tacggggtta aattgcaaag ttccttagga agtgttctct
       61 cacgcccctt ggtgtattcc acccacctac ctgtgtcggt ttgcggtacg gacacaatga
      121 tgactcgcta cgaggctttt ctcggcagca tgggatcagc ccgtttacgg ccttgcggcc
      181 tccccatcac gtctcggcga taacggcctt gcggatttgc cggcaagacc cgcctacgcg
      241 cttagaccgg gtattccagg gacccggcgg tgcctaccct tctgcacccc cccttcgctg
      301 ataacgccac cactgtggta caggaatatt gacctgtttg ccatcgccta cgcctctcgg
      361 cctcggctta ggatccgact caccctgacc tgacgaacat aggccaggaa accttaggtt
      421 tacggggttg atgattctca catcaattat cgctacttat gcctgcataa tctcttctct
      481 gcgctccagc tgtccttgtc ggtcaacctt cgctgcacag agaatgctcc cctaccactc
      541 acctcttgcg aggaaagtcc gtagcttcgg tggcaaactt gagccccgat aaattttcgg
      601 cgcaccatcg ctcgaccagt gagctattac gcactcttta aaggatggct gcttctaagc
      661 caacctcctg gctgtctgag cgacgttaca acctttccca cttagcttgc gcttagggac
      721 cttagctgac ggtctgggct atttcccttt tgacaacgga tcttagcact cgtagtctca
      781 ctcccgtgcg tccagtaacg gcattcggag tttgattggg ttcagtagcg ttggaacgcc
      841 cctagcccat tcagtgctct acctccgtta cggctgacac gaggctgtcc ctctagacat
      901 ttcggggaga accagcaatc acgaagtttg tttagccttt cacccctacc cacagctcat
      961 ccgagctttt tgcaacaaac atcagttcag gcctcctccg cctgttacgg cggattcgcc
     1021 ttggccatgg gtagatcact tcgcttcggg tctatcctac gcaactaaat gcccaattcg
     1081 gactcgcttt cgctacggct ccggctttcc gccttaacct tgctacgcag gataactcgc
     1141 aggctcatta agcaaaaggc acgcgatcag gcattccctt gcgggcatag ccctttcgct
     1201 gcttgtaggt gtacggtttc aggttctatt tcactcccct caccggggtt cttttcacct
     1261 ttccctcacg gtactggtgc gctatcggtc atcagcgagt atttagcctt ggaacgtggt
     1321 cgtcccggat tcccacaagg tttctcgggc cccgtggtac tcaggacctc tatccaacga
     1381 gtcagggtct tgtcgcttac ggggctgtca cccgctttgg ccggactttc cagaccgttc
     1441 agctaagatc ctgattgatc actcgtcgac ggggccgtat ccccgtccga caaaggcctt
     1501 caacccctta tctacaacgc acacgggctt gacatagata aggtttaggc tg
//
LOCUS       DATMTI010000381         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_651227, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000381 DATMTI010000000
VERSION     DATMTI010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651227"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /gene="lipB"
                     /locus_tag="VN039_06250"
     CDS             <1..703
                     /gene="lipB"
                     /locus_tag="VN039_06250"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWV45607.1"
                     /translation="NTLTSPDLSSSSIHAGAVRIFSDPVPYLIAWEIQSRLHEERLLD
                     IQPDTVLILEHPPVYTLGRRTSLSHWGGSESALCADGAELHRVNRGGSVTFHGPGQIV
                     VYPVLKLERHAAGPRRLIWLLEEVIIRVLDHWNITGSRVVGKPGVWIMTPEPQKIAFL
                     GIRIEHGVTLHGFALNVDLALTPFQRIHPCGFPDCRVTSMAAVRQTAVPIGVVKQELA
                     QTFAAVFDLNWSTVA"
     gene            749..1999
                     /locus_tag="VN039_06255"
     CDS             749..1999
                     /locus_tag="VN039_06255"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWV45608.1"
                     /translation="MRELDTPTFRLAVAQFDQAAEAMGLDQTLRERLKLPQRSLVVSL
                     PVRMDDGHVEVFTGYRIQHDSSRGPSKGGVRYHPDVNLGEVAALAMWMTWKCALAGLP
                     YGGAKGGVAVAPKQLSSAELQRLTRRYAAEIFPLIGPDKDVPAPDVGTDAQIMAWMMD
                     TYSQQVGYAVPGVVTGKPLSIGGSLGREEATGRGVVYVTQEVLRHLKLSIENATVAIQ
                     GFGNVGSHTARIMQQQGARVIAVSDVHGGIYNPKGLDVMELLRRDPSQPLHNAKLGDA
                     VTNEELLQLECTVLVPAALSEQITSKNANSLRCRILSEGANGPTTLEADRILAEKGIF
                     VIPDILANSGGVIVSYFEWVQDLQRFFWSATDIQNRLQGIITSAFQRTLHFSTERRVS
                     MRMAALMSGIDQVAQAHLQRGLYP"
     gene            2128..2409
                     /locus_tag="VN039_06260"
     CDS             2128..2409
                     /locus_tag="VN039_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45609.1"
                     /translation="MIVTAWEQHCADGWPQFSSPDQGQLMTIDTVISGCVVYFLDSSE
                     GLDEQRVAIVKDCLGDLDELTEALDTGSQAYFYRLRKLGAMLLGDEPQS"
     gene            2583..>2902
                     /locus_tag="VN039_06265"
     CDS             2583..>2902
                     /locus_tag="VN039_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45610.1"
                     /translation="MTNARVIRMAIILLASILATVGSAHAEPYELSKNDVMEPKVISS
                     AEISLFGVKLGDSESKAVETLVNEKISGIKAEQEATFIFLLDQRKPIGPMAGVRIQDG
                     KVDLL"
BASE COUNT          653 a          783 c          810 g          656 t
ORIGIN      
        1 caataccctg acgtctccgg atttgagctc ttcctctatt catgcgggcg cggttcgcat
       61 cttttcagat ccggtcccct atctcattgc gtgggaaata cagtctcgct tgcatgagga
      121 gcgtctcctc gatatccaac cggatactgt tctgatcctt gaacatccac ccgtctatac
      181 gctcggacgc aggaccagtt tgtcgcattg gggaggcagt gaatccgcgt tatgcgcgga
      241 tggcgcggaa ctgcatcgcg tcaatcgagg aggctctgtg acctttcacg gcccaggaca
      301 gattgtggtc taccccgtgc tcaagcttga acgacatgca gcgggaccca ggcgattgat
      361 ctggttactg gaagaagtga tcattcgggt actcgatcac tggaacatta ccggctcccg
      421 tgtcgtaggt aaaccaggtg tgtggatcat gactccggag ccacagaaaa tcgcattcct
      481 gggcatacga attgagcacg gcgtgacatt gcacggattt gctctgaatg ttgatttggc
      541 cctgacgccg ttccaacgga tccatccctg tggatttcca gattgccgag tgacatccat
      601 ggcggcagta cgccaaacag ccgtcccgat tggcgtcgtc aagcaggaac ttgctcagac
      661 cttcgcggcg gtatttgacc tcaactggtc tactgttgca tgaccagccg acgtacgaaa
      721 caggctggtg tttgcgtaga ggagtcctat gcgggaactt gataccccga cctttcgcct
      781 ggccgtcgcg cagttcgatc aggcggcgga agcgatggga cttgaccaaa ccctccgtga
      841 acggctgaag cttccacagc ggtctctcgt cgtcagcctt ccggttcgga tggacgacgg
      901 gcacgtggag gtttttaccg gctaccgcat tcaacatgat tcgtcacggg gcccgtccaa
      961 gggcggcgtg cgttaccatc ccgacgtgaa cctgggtgag gtggccgctc ttgcgatgtg
     1021 gatgacgtgg aaatgcgcgt tggccgggtt gccgtacgga ggcgcaaaag gcggagtcgc
     1081 agtagctccg aaacaactgt cctctgccga gttacagcgg ttgacacgac gatacgccgc
     1141 agagattttc ccgctgattg gcccggacaa ggatgtaccg gccccagacg tgggaaccga
     1201 tgctcagatc atggcatgga tgatggacac atacagtcaa caagtcggct acgccgttcc
     1261 tggagtggtg accggcaaac cgctctcgat cggagggagc ttgggccgtg aagaagcgac
     1321 gggacgcggg gtcgtctatg tgacgcaaga ggtgcttcgt caccttaagt tgtccattga
     1381 aaatgccacc gtggcaattc aaggattcgg caatgtcggt tcacacaccg cccgcatcat
     1441 gcagcaacaa ggtgcacgcg tcatcgccgt cagcgatgta catggtggaa tttacaaccc
     1501 caaggggttg gatgtcatgg agttgcttcg tcgcgatcct agccaaccgc tgcataacgc
     1561 caaactggga gatgcggtca caaatgagga acttctgcaa ctagaatgta ccgttctcgt
     1621 acccgccgca ctctcggaac agattacaag taaaaatgcc aattctttga ggtgtcggat
     1681 tttgtcggaa ggagcgaacg gtccgacgac gctggaggcc gaccgcatcc ttgcagagaa
     1741 aggcatcttc gtaatccccg atattctcgc caactccggc ggcgtcattg tctcatactt
     1801 cgaatgggtg caggaccttc agcgattttt ctggagtgca acagacatcc agaatcggct
     1861 gcaaggcatc atcacgtccg cctttcagcg gacacttcat ttttcgacgg aacgccgagt
     1921 atcgatgcgt atggcagccc tcatgagtgg gattgatcaa gttgctcaag cacatctcca
     1981 acgtggactg tatccctgat cctgcaattc ctccaggccg tatcatcgga atatcctggc
     2041 tgatacttgt ccctctgcta ccggatctat ttgggggctg gcctgtcaca tggcggagag
     2101 ggaaaggagt acctcatgga tcgaagcatg attgtaacag cgtgggagca acattgtgcc
     2161 gacggttggc ctcagttttc gagcccggat caagggcaat tgatgacgat cgacacagtc
     2221 attagcggct gtgtggtcta ttttctcgat agttccgaag gcctcgacga gcaacgggtt
     2281 gccatcgtga aagactgttt gggagatctc gatgaactga cggaagcatt ggacaccggg
     2341 tcccaagcct acttttaccg gcttcgtaaa ctaggggcga tgttgctggg cgatgagcca
     2401 cagtcatgat gcgaggatac cgtgtccacc cgacgagtcc cttatccaat ggccgctcgc
     2461 gcatcccgtt ccttgcacgt ctagcggctc ttggcataca atcgtcacat gttcatctcg
     2521 attctatcga ttcacccatc ctgatcaagt cacgaaccca tacgaaagaa acgctaaata
     2581 ccatgacgaa cgcgcgtgtg atacggatgg caattattct actggcgagc atcctagcaa
     2641 cggtcggctc agctcatgcc gagccgtatg aattaagcaa gaacgatgtc atggaaccaa
     2701 aggtcatttc gagcgctgag atttcgctgt tcggcgtcaa actcggcgac tcggaatcca
     2761 aagccgtcga aacactcgtg aacgaaaaga tttccggaat caaggctgag caggaagcga
     2821 cgttcatctt cctgcttgat caacgaaaac cgatcggtcc aatggccggg gtgcgtattc
     2881 aggacggtaa ggttgacctc ct
//
LOCUS       DATMTI010000382         1545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_438739, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000382 DATMTI010000000
VERSION     DATMTI010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438739"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN039_06270"
     CDS             <1..322
                     /locus_tag="VN039_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45611.1"
                     /translation="MTRTDDAAQIPSVRSILPRCRFNNTPSVKRGLECLRHYHNKAKV
                     EGGKTSWSPRPVHDWSSHGCKAFATGAYFAPELRAGVQPPKQPAKDLFANYQHSYEVG
                     GWMR"
     gene            405..>1545
                     /locus_tag="VN039_06275"
     CDS             405..>1545
                     /locus_tag="VN039_06275"
                     /inference="COORDINATES: protein motif:HMM:NF027825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HWV45612.1"
                     /translation="MQEEDGTYSADSTASSPSDDSEQADHSELIKLAVDRFKQAYDAH
                     KDDFEQCERVQEFISGDQWPTAIKAERERLGRPCLTLDLLTQYVRHVINTGLLRYRDI
                     RVLAMDGMADDEVADIIAGIIRQITQTSTAKVAYETGLRHSCSVGFGYWRVRVQNVAG
                     SPLEEIVIHKIREPRMVFLDPHCLYPDGRDARYAFVLTKMQRSEFKEQYPGHENATSW
                     HDVHEQSILPWTGDDVIVLAEYYYTKTYTDEMGQEKSGLFFTIMTPEYVLEEGKHHGD
                     AMPIIRVVGDEYEGKGKERKRGMINASSMDAQRTFNYANSAFIENVALAPIAPWVAAA
                     KQVDQHVKEWEEAHRVPRSVLTYDPIIVNGTLLPPPTRSMPAGIPQ"
BASE COUNT          366 a          426 c          425 g          328 t
ORIGIN      
        1 catgacccga accgatgatg cagctcaaat cccaagtgtc cgctctatcc tcccgcgctg
       61 tcgatttaac aatacgccaa gcgttaagcg tgggcttgag tgtctacgtc attaccataa
      121 caaggcgaaa gtcgaagggg gcaaaacctc gtggagccct cgtcctgtcc acgattggtc
      181 gtcacatgga tgtaaagcgt ttgctacggg ggcctacttc gcgcccgagc tgagggctgg
      241 cgtccagccc cccaagcaac cggccaagga tctctttgcc aactatcagc atagctatga
      301 agtgggcgga tggatgcgct aatgctacct ttcggtaaca cctagttgac agaagtgtgt
      361 cgttacggta acatcctgca tcttacgtta cgaaaaggta acgaatgcag gaagaagacg
      421 ggacgtattc cgctgattca accgcctcct ctccctctga cgattcagag caagccgatc
      481 attccgaact cataaagctc gcagtcgacc gcttcaagca agcctacgac gctcataaag
      541 atgatttcga acaatgcgag cgcgtccaag agttcatctc gggcgatcaa tggccgactg
      601 cgatcaaggc cgagcgtgaa cgcttaggtc gtccctgcct caccctcgat ctcctgaccc
      661 agtatgtgcg ccatgtgatt aataccggcc tcttgcggta tcgcgatatt cgtgtgctcg
      721 cgatggatgg catggccgac gatgaagtgg ccgacattat tgcggggatt attcgccaga
      781 ttacgcaaac ctcgaccgcg aaggtcgcgt atgaaaccgg actccggcac agctgctcag
      841 taggttttgg ctattggcgg gtgcgtgtcc aaaacgtcgc aggctcgccg ctcgaagaaa
      901 tcgtcattca caagatccgt gaaccgcgca tggtgtttct cgatccgcat tgtctctatc
      961 cggatgggcg ggacgcccgc tacgcctttg tactcacgaa gatgcagcgc agtgaattca
     1021 aggaacagta tccagggcat gagaacgcca caagctggca cgatgtgcac gagcaatcga
     1081 ttcttccatg gacaggcgac gatgtgattg tcctggccga gtattactac accaagacct
     1141 atactgacga gatgggccaa gaaaagagcg gtctcttctt cacgatcatg accccggaat
     1201 atgtgctgga agaagggaag caccatggcg atgccatgcc gatcattcgg gtcgtcgggg
     1261 atgaatatga aggcaaaggc aaagagcgga agcgcgggat gatcaacgcc tcgtccatgg
     1321 atgcgcagcg cacatttaat tatgccaatt cggcctttat cgagaatgtg gccctcgcgc
     1381 cgattgctcc atgggtcgct gcggccaagc aggtcgacca gcatgtgaag gaatgggaag
     1441 aggcgcaccg ggtgcctcga tcggtgctga cctatgatcc gataatcgtc aatgggacgc
     1501 tacttccgcc tccgacccgt tccatgcctg ccgggattcc gcaag
//
LOCUS       DATMTI010000383         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_693221, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000383 DATMTI010000000
VERSION     DATMTI010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693221"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1249
                     /locus_tag="VN039_06280"
     CDS             326..1249
                     /locus_tag="VN039_06280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWV45613.1"
                     /translation="MKAIRLHQRGGPEALLYEETPRPIQKDNQVLVQVYATAITPTEF
                     DWYPTFHTPEGGTRPFPIILGHEFSGVVEAVGSDCLGIQVGDPIYGLSDWFIDGAQAE
                     YCLTVPANIAPKPVTLEHIQAAAVPISALTAWQALIDRAHLSSGQRVLIHGAAGGVGS
                     FAVQLARHQCAHVIATASAANIDFVKALGADEVIDYQITSFETVVGDVNVVLDTVGGD
                     TRDCSWGVLKNSGRLITIASDAEGAKEQRVRDAFFIVEPNRNQLIDISRLIDTGVLRP
                     AVGVVYSMENFRQAYEQKPGRGKNVLRIAEK"
     gene            1285..1728
                     /locus_tag="VN039_06285"
     CDS             1285..1728
                     /locus_tag="VN039_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV45614.1"
                     /translation="MMTIAPIRTVTRQTLQWMALAGATLLLGQTALAEDSVSVLIRQR
                     LADMAGKEGTVITVDYAPGAASSQHFHPGSVFAYVLEGAVVSQLGGEQPVTYTKGQSW
                     YESPKEPHVVSKNASKTEPAKLLVFLLSQESESLVVPMKSLGSGK"
     gene            1965..2468
                     /gene="msrA"
                     /locus_tag="VN039_06290"
     CDS             1965..2468
                     /gene="msrA"
                     /locus_tag="VN039_06290"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWV45615.1"
                     /translation="MSRTETAILAGGCFWGMQDLIRKLPGVLSTRVGYSGGDVPNATY
                     RNHGTHAEAIEVVFDPDMLEFRGLLEVFFQIHDPTTVNRQGNDQGTSYRSGIYYTSEA
                     QRMVAEETITDVNASGLWPGKVVTEVRPAGPFWQAEPEHQDYLERYPNGYTCHYARPN
                     WKLPRRA"
BASE COUNT          586 a          653 c          755 g          533 t
ORIGIN      
        1 tgatgtgtct tgggagaaag ggctagagaa tgaggtcgag taaatatgta ctagggacgt
       61 tgctagtact ttggtgtatc tactcgcacc atactcggag cccattgtcg gtcaatcacc
      121 atggctcagg atcgaacacg aaccgcccca cgcggtgcgt gccttcaagg ttgttgtcaa
      181 agcagcagac gcaattctca tcgtgacccc gggtgatcag aactcgggac ggacgaattg
      241 tcgaggagcg tgtcaatcca cgcgaatcct ttccgaccgc cttgctgcac ccccatgaag
      301 taagcgacgc gatgaggctc ttcccatgaa agcgatacgg ttacatcaac gtggtggccc
      361 cgaggcgctt ctctatgagg agacaccgag accgattcag aaggacaatc aagtgctcgt
      421 tcaggtctat gccacggcaa tcacaccaac ggagtttgac tggtatccaa cattccatac
      481 tcctgaaggt gggacgagac cctttccgat tattctgggt catgaattct cgggtgttgt
      541 cgaagcagtc gggtcggatt gcctgggtat tcaggtgggg gatcctattt acggactgag
      601 cgattggttc atagacggtg cgcaggcgga atattgtttg accgttcctg ccaacattgc
      661 accgaagcct gtcacgcttg aacatattca agccgcagcg gtcccgatct cagcactcac
      721 cgcttggcag gcgcttatcg atcgcgcaca tctctcgtca ggacaacggg tgttgatcca
      781 tggtgcggcc ggtggtgtgg gcagctttgc ggtgcagctg gctcggcatc agtgcgcaca
      841 cgtgatcgca acggcttcgg cagcgaacat cgattttgtc aaggcgctcg gcgccgatga
      901 agtcatagat tatcagataa cgtcgtttga aacagtcgtg ggagacgtga atgtggtgct
      961 tgatactgtc ggaggagaca cgagagactg ttcgtgggga gttcttaaaa atagtggtcg
     1021 cttaatcacc attgcgtcag atgcagaagg ggcgaaggag cagcgggtgc gcgatgcctt
     1081 cttcatcgtc gagccgaacc ggaatcaact gatagacatt tcgcgtctga tcgataccgg
     1141 cgtcctccga ccagccgtgg gtgtcgtgta ctcgatggag aactttcgac aggcctacga
     1201 acagaaacct gggcgcggca agaatgttct ccgcatcgcg gagaagtgat ggatggatca
     1261 taatgaacaa gaaggagcac gccaatgatg accattgcac ctatccgcac tgtgacaaga
     1321 cagacacttc agtggatggc cctggcaggg gcgacgctgc ttctgggaca gacagcgctg
     1381 gcagaagata gcgtgtcggt gctgatacgg cagcggttgg cggatatggc cggcaaagaa
     1441 ggcactgtga ttaccgtgga ctatgcaccg ggcgccgcat ccagccagca cttccatccg
     1501 ggctcggtgt tcgcctatgt cttggaggga gccgtggtct ctcaattagg aggagagcag
     1561 cccgtgacct atacgaaagg tcagagctgg tacgagtctc ccaaggaacc gcatgtagtg
     1621 tccaagaacg cgagcaagac cgaaccggcc aagttgctgg tgtttctgct gtcgcaggag
     1681 agcgagtcgc tcgtcgtgcc gatgaaatca ctcggcagcg ggaagtgaac caagctatgg
     1741 tacctgaagc acgtgtctca gctgactact gaacgggggt ttcacccaac ccgtggcccc
     1801 gagcgtgatc gcctatcatc aggacgacag tcatggtatg cgccggatcg agacaacgtg
     1861 caatgtgtgc gatgctcatc ttggccatgt gtttccaagg ggtgatccgc cgaaatagac
     1921 ggcttccgac gcagggacag gcacacgacg aaaggagtat ttccgtgagc cgaactgaaa
     1981 cagcgatatt ggccggcggt tgtttctggg gcatgcagga tcttatccgc aagctccccg
     2041 gtgtgctttc tacgcgcgtt ggctacagcg gtggtgacgt gccgaatgcc acctatcgta
     2101 accatgggac gcatgcggaa gccatcgagg tcgtgttcga cccggacatg ctggagttcc
     2161 gtggcctact ggaagtgttt ttccaaattc acgatcctac gacagtgaac cgccagggta
     2221 acgatcaagg aacatcatac cgttcgggaa tctattacac ctcggaagcc caacgcatgg
     2281 tggcagaaga gacgatcacc gacgttaacg cgtctgggct gtggccggga aaagtagtca
     2341 cagaagtccg gccagccggt ccgttctggc aggcggaacc ggaacatcag gattatcttg
     2401 aacgatatcc caacggatat acctgtcact acgcgagacc caactggaag ttaccgcggc
     2461 gggcctgagg tacaacgcct ttgctgcacg cgctactcta cgagctgcac gaggggagaa
     2521 taaatct
//
LOCUS       DATMTI010000384         1957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_141995, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000384 DATMTI010000000
VERSION     DATMTI010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141995"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..>1957)
                     /locus_tag="VN039_06295"
     CDS             complement(48..>1957)
                     /locus_tag="VN039_06295"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWV45616.1"
                     /translation="TPPPYSIPKGSPMAGLAASPELLSALHNKATQLRINSVRATSEA
                     GSGHPSSCASAADIVAALFFSVMRYDPHNPKAPNSDRFILSKGHAAPLLYAAWAEAGL
                     FPQNDLLQLRTLTSDLEGHPTPRLPFVDMATGSLGQGLPVGIGIALNAKFVDRLDYRT
                     YVLMGDGESVEGSVWEAAEIGRQYGLDNLCAIVDVNRLGQSDPTMLQHDMDAYRSRWA
                     GFGWHAIVVDGHDFTAILKAFDEAARTKGTPTVLLARTYKGKGISFIENKDGWHGKPL
                     KKGEETQKALDELTKQLSPNGVVIQIPKPSAPDTAPTAIRPMPPSPYKIGDSVATREA
                     FGVALEALGSVNPSIVALDADVKNSTYTDKFGKKFSNRFFESFIAEQNMIGAAAGLAA
                     CGKIPFAATFACFFSRAYDFIRMAAISGSNIKLVGTHVGVSIGEDGPSQMGLEDIAMM
                     AAQPNVTVLYPSDGNSTYQLIEEAAKHKGMVYIRAGRPKTPVLYGPEERFRIGGSKIL
                     RQSAADVLTITAAGVTLFEALKAYDQLKSSGIAVRVIDLYSIVPIDRNSLLESGRATQ
                     RRILTVEDHYAHGGLGDAVLSAVATEGMKVFKLAVREIPHSGKPEELVDHYGIGVRSI
                     VEATKRIVREP"
BASE COUNT          414 a          527 c          555 g          461 t
ORIGIN      
        1 gtttctcagg cggacggttt ttgttcgggc cgttccatga tgcaatgtta tggttctcgg
       61 acgattcgtt tggttgcctc aacgatggac cgcactccaa tcccatagtg atcgacgagt
      121 tcttcaggtt ttccactatg cgggatttca cgaacggcga gtttaaagac tttcatgcct
      181 tccgttgcga cagcgctaag aacggcatcg cccagacctc cgtgggcgta gtgatcctca
      241 accgtgagga ttcgacgctg cgttgcgcga ccgctctcca gcaagctatt tctatcgatg
      301 gggacgatgc tgtaaagatc gattacacgg acggcaatgc ccgatgactt cagctggtca
      361 taggctttca aggcttcaaa cagtgtcact cccgccgccg taattgtgag cacatccgct
      421 gcgctctgcc ggaggatttt gctgccgccg atgcggaacc gttcctctgg tccgtagagg
      481 accggagtct ttggccttcc agcacggata tagaccattc ccttatgttt cgcagcttcc
      541 tcgattaatt gatacgttga gttgccgtct gaaggataga gcaccgtcac gtttggttgt
      601 gccgccatca ttgcgatatc ttccaagccc atttgcgacg gcccatcctc gccgatgctt
      661 acgccgacat gagttccgac gagcttaata ttcgagccac tgatcgcggc catacggatg
      721 aagtcatagg cgcgactgaa gaaacaggca aaggtcgctg caaacgggat tttgccgcag
      781 gcagccagac cggcggccgc accgatcata ttttgttcgg cgatgaaact ctcaaagaat
      841 cgattggaaa acttcttgcc gaatttgtcg gtgtacgtgg aattttttac gtcggcgtct
      901 aatgcgacaa tcgaggggtt cacggacccc aaggcttcca atgccacacc gaaggcttca
      961 cgggtggcga cggagtcacc gattttgtaa ggtgagggag gcatcgggcg gatggctgtg
     1021 ggagcggtat cgggagccga tggctttggg atctgaatca cgacgccgtt tgggctcaat
     1081 tgcttcgtga gctcgtcgag ggccttctga gtctcctcac ctttcttaag cggtttgccg
     1141 tgccagccgt ctttgttctc gatgaacgaa atgcctttgc ctttgtacgt tcgggccagc
     1201 aacacggtcg gcgttccttt tgtccgggca gcctcatcga atgccttgag gatggctgtg
     1261 aaatcatgac cgtccacgac gattgcatgc caaccgaatc cggcccatcg ggaacggtaa
     1321 gcgtccatgt cgtgctgcag catggtggga tcactctgcc ccagtctgtt cacatcaaca
     1381 atggcgcaca aattatccaa gccgtattgg cgaccgatct cagcggcttc ccaaaccgat
     1441 ccttcgaccg attctccatc ccccatcaac acgtaggttc ggtaatcgag cctgtcgacg
     1501 aacttcgcat tcaacgcgat gccgatccca acgggaagtc cttgccccag tgaaccagtg
     1561 gccatatcga caaagggcaa gcgaggagtc ggatgtcctt ccaggtcgga cgtcaaggtg
     1621 cgcaattgta agaggtcgtt ctgtgggaag agacccgctt ccgcccacgc cgcatagagc
     1681 agcggagccg catgtccttt tgacaggata aaacggtcgc tgttgggggc ctttggatta
     1741 tggggatcat aacgcatgac ggaaaaaaac aatgcggcga cgatgtcagc ggcggaggca
     1801 cagctcgacg gatggccgct cccggcctcg ctggtggccc gcacactgtt gatacggagt
     1861 tgggttgcct tattgtgcaa ggcactgagt agttcaggcg aagcagctaa accggccatg
     1921 ggagatcctt tcgggataga gtaaggaggt ggagtat
//
LOCUS       DATMTI010000385         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_14910, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000385 DATMTI010000000
VERSION     DATMTI010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14910"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VN039_06300"
     CDS             <1..458
                     /locus_tag="VN039_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45617.1"
                     /translation="NDNKRGIAFGVAGGALASVFLVAILTVIPGLNRYAIAPPQELAY
                     AAGLNLHPTDQLIAFGSTRPSIAFYARRTVTFIPAGEIDRLRTALSKEGRTMILLPEY
                     FQDALPREAAEFQPILKRYGYVLLGNQQIITMPPGADVGALPPAKTLGH"
     gene            complement(533..1621)
                     /locus_tag="VN039_06305"
     CDS             complement(533..1621)
                     /locus_tag="VN039_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWV45618.1"
                     /translation="MSALSRSSPNTILSIAAAGCSCAALAVSFLGLAQFDLPITKYVR
                     SVTIHLPWDQLTIPWMAFTSDTGDWIGQGWRLAALSLLLLLGAWALEKPTIKIVAIQS
                     LIAHGIAALLANGLKHLVGRPRPKFVHSGDWQMTLTWASGLDSFPSGHSTATFAVATV
                     LAKRFPLLGPLCIAIALFVALSRVLRGSHFPTDVLGGAVIGILSGFIATAPVRQWRTS
                     MQDGLRHAAMGMSALFALLWVLSRRMEDGMVGILFLTLGAAAVAGGLWIRRTYWVRRG
                     GIGVSRLSDISALLMAYGLAAMTISPLVISSVGFVCMASWLHALGRNEDHGEESLSRF
                     VMRESVLIGGLVLAILILIDGRGVLPFQ"
     gene            complement(1697..2821)
                     /locus_tag="VN039_06310"
     CDS             complement(1697..2821)
                     /locus_tag="VN039_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45619.1"
                     /translation="MQRRKTERMWLWRLCALMSIPWFAMAADQPNEPVTLAPSTERHL
                     ANIRQLTFGRQNAEAYFSFNGTKLIFQSTNNWMKGSSPSARKPAESGLGCYQMYVMDV
                     ENETVRLVSTGKGATTCGYFFPGDRRVLYSSTHAAGPDCPPKPKRDGAYRWALDDYDI
                     YSANLDGQYIQRLTASPGYDAEATISPDGKAVVWTSVKDGDLDLYTMNLDGSNVRRLT
                     DDVGYDGGAFFSPDSKKLVYRAAHPTDPSEVAKYKELLAQRLVEPGQLEIFVMNADGS
                     GKKQVTTTGASNFSPYFHPDGTRIIFSSNIETRGEGGRPSFHLYLVRDDGTGLERLTM
                     EGRFNSFPMFSPDGKRLVWVSDRNAKEPGEFNVFLADWAP"
     gene            complement(2808..>3067)
                     /locus_tag="VN039_06315"
     CDS             complement(2808..>3067)
                     /locus_tag="VN039_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45620.1"
                     /translation="GGKIDGQAYDGPKMAVLFSCHRANVPGSVITVLYGVTSGAVEKM
                     SRFLFYYGWHSYVIFQDGAVTKRGVWQGTSDVKEVRIDATS"
BASE COUNT          713 a          925 c          787 g          642 t
ORIGIN      
        1 taaacgacaa caaacgtggt atcgcctttg gagtggccgg cggcgccctg gcgagtgttt
       61 ttcttgtcgc catcctgacg gttattccag ggctcaaccg atatgcgatt gcgccgccgc
      121 aggaattggc ctatgcggca gggctcaacc tgcatccgac cgaccaactg atcgcgttcg
      181 gctcaaccag gccatccatc gccttctacg cacggcgaac ggtgaccttt atcccagccg
      241 gagaaatcga ccggttacgt acggctttga gcaaggaagg tcggacgatg atcctattac
      301 ccgaatactt tcaggatgcc ctgccaaggg aagcggctga atttcagcct attctcaagc
      361 gctacggcta tgtcttgttg ggcaatcaac agataatcac catgcctcct ggcgcagacg
      421 ttggtgccct accgcctgct aagactcttg gtcactgagt tccactcgac tccgcttttt
      481 agggttctct ttctgaatgg aatactgaac gtagctcttt cgcaatgatt cgttactgaa
      541 agggcagaac ccctcgccca tcaatgagaa tcagaatggc aaggaccagg cccccgatta
      601 gaacgctttc ccgcatcacg aatcggctaa gcgattcctc accgtggtct tcgttccgac
      661 ctagcgcgtg gagccatgac gccatgcaaa cgaatccaac cgacgagatg acaaggggtg
      721 agatcgtcat ggcagccagg ccgtacgcca tcaacagcgc tgaaatatcc gatagccggc
      781 tcactcctat tccccccctg cgaacccaat aagttcgccg gatccataaa ccgcccgcta
      841 ccgcagcagc ccctagcgtc aagaacagga tccccaccat tccatcctcc attcgacggg
      901 atagaaccca gagcaaggca aacaatgccg acatccccat cgccgcatgt cgtagtccat
      961 cctgcattga agtgcgccac tgtcttaccg gggctgtagc gatgaatccg ctgaggatgc
     1021 cgatcaccgc ccctccaagg acatcggtcg ggaaatgcga tcctcgaagg acacggctga
     1081 gtgctacgaa aagagcaatc gcgatacaga gtggtccgag gagagggaac cgtttagcca
     1141 gcaccgtcgc gacggcaaaa gtcgccgtgc tatgtcctga tggaaaagag tccagccccg
     1201 atgcccaggt aagggtcatc tgccagtctc ccgagtggac gaacttcggt cggggccttc
     1261 cgacgaggtg tttcagcccg ttggcaagca atgcggcgat gccatgggcg atcagggact
     1321 ggatagcaac tatcttgata gtcggctttt caagggccca cgcgccgagg agcagtagaa
     1381 ggctcaaggc tgccagccgc cagccttggc cgatccagtc ccccgtatcg ctggtgaacg
     1441 ccatccacgg aatggtgagt tggtcccagg gtagatgaat ggtcaccgat cgcacatact
     1501 tggtgatcgg cagatcaaac tgagccaagc cgagaaagct gacggccaac gcggcgcagg
     1561 aacaaccggc tgccgcgatc gacaagatcg tattcgggga tgaccgagac agggcactca
     1621 tcaagccacg atctgtaacg agggtccctg gacttccgga actgatcacc gatcgctcat
     1681 cgccgattac cgcgcctcac ggcgcccaat cggccaggaa tacgttgaat tctcccggtt
     1741 ccttggcatt tcgatctgat acccagacga gccgtttgcc gtccggggaa aacatgggaa
     1801 aactattaaa gcgtccttcc atggtcaaac gttctaaccc ggttccgtcg tctcgcacca
     1861 gatacagatg aaaactgggg cgcccccctt cccctctcgt ttctatgttg gaggaaaaga
     1921 tgatgcgtgt gccgtcagga tgaaaatacg gagaaaaatt cgacgcgccg gtcgtggtga
     1981 cctgtttctt gcccgaccca tcggcattca tgacgaaaat ttcaagctga ccgggctcga
     2041 ccagtcgttg tgccagcaac tccttgtatt tggcaacttc ggacggatcg gttggatgcg
     2101 cggcacgata gacgagcttt ttgctgtccg gggaaaagaa cgctccgccg tcgtatccga
     2161 catcgtccgt caggcgacga acgttcgagc cgtccagatt catcgtatac aggtcaagat
     2221 ctccatcctt caccgatgtc cagacaacag ccttcccgtc aggtgagatc gtggcctcgg
     2281 catcatatcc tggcgatgcc gtcagcctct gtatgtactg tccatcaaga ttcgctgagt
     2341 agatgtcata atcgtcgagc gcccatcgat aggcaccatc tcgtttgggt tttggcggac
     2401 aatcgggccc ggcggcatgt gtggatgagt acagcaccct gcggtcgcct gggaaaaaat
     2461 agccgcatgt cgtggcgcct ttcccggtac tcaccaaacg gacggtctcg ttttccacat
     2521 ccatcacgta catttgataa caccccagtc ccgactcggc tggtttccgt gctgaaggcg
     2581 acgaaccctt catccagttg ttcgtcgatt gaaaaatcag cttggtccca ttaaacgaaa
     2641 aataggcctc cgcattctgg cgaccaaaag tcagctggcg gatattggcc agatgccgtt
     2701 ccgtagaagg agccaatgtc acaggctcat tcggctgatc agcggccatg gcaaaccagg
     2761 gaatgctcat caatgcgcac aacctccaca gccacatcct ctccgtctta cgacgttgca
     2821 tcaatccgta cctccttcac atccgacgtc ccttgccata ctccgcgttt cgtcacggct
     2881 ccgtcttgaa aaatgacgta actatgccac ccataataaa acagaaaacg cgacattttc
     2941 tcgaccgcac ctgaggtcac gccatagaga accgtgatga cgctgcccgg cacattcgcc
     3001 cgatggcagg aaaatagcac tgccatcttc ggcccatcat aggcctgacc atcaatcttt
     3061 cctcccc
//
LOCUS       DATMTI010000386         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_481961, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000386 DATMTI010000000
VERSION     DATMTI010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481961"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(711..983)
                     /locus_tag="VN039_06320"
     CDS             complement(711..983)
                     /locus_tag="VN039_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45621.1"
                     /translation="MARHNTFALDSETAAHAERMRDVLKTADSAGRGVSFSVQGRDSG
                     TVTYRTGTVIGFSGDYGMSTETVLVDTDKGPRSFNVWLIRSIQIGE"
     gene            complement(1137..1358)
                     /locus_tag="VN039_06325"
     CDS             complement(1137..1358)
                     /locus_tag="VN039_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45622.1"
                     /translation="MYLTDETMDELAQWQHDTAWETPESLDPEVIHESDIADCVCDDG
                     RLHEYADKAGVFFKVWECNDCGKLVEVKA"
     gene            2038..2214
                     /locus_tag="VN039_06330"
     CDS             2038..2214
                     /locus_tag="VN039_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45623.1"
                     /translation="MTKKLSSRSIAVAEAIRDREPFETYGTLSATRHMGYAQPGRLSG
                     PDLDAFNRDQGSFS"
     gene            2224..2358
                     /locus_tag="VN039_06335"
     CDS             2224..2358
                     /locus_tag="VN039_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45624.1"
                     /translation="MEQKVWYYNSILRILRRSNCTEERAAELMNIHTLPYLIYFVEEV
                     "
     gene            2474..2743
                     /locus_tag="VN039_06340"
     CDS             2474..2743
                     /locus_tag="VN039_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45625.1"
                     /translation="MTFTPANLVANRTARLVAEATTPVYSTDTAGAAAKERLRKLEWA
                     EEFTHSEDDRQPWCHVCSRPTDHFGEHSDEQLLAFYNGQGRKFRR"
     gene            2944..3138
                     /locus_tag="VN039_06345"
     CDS             2944..3138
                     /locus_tag="VN039_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45626.1"
                     /translation="MTRKHYIATAAMFADMMDGMSIIGSNFIAGYARAIVDAANNMAD
                     IFEADNPRFDRDKFIKACGL"
     gene            3201..3554
                     /locus_tag="VN039_06350"
     CDS             3201..3554
                     /locus_tag="VN039_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45627.1"
                     /translation="MKTLILAIVMVFALACPASADQTPGVVTVHEFNHAQKGWTRAQV
                     TKSFGTHGHAIGGNWIQGRFILTVKYHGINGPTFANFVDRGTTGDNFHLRNLYRFTKT
                     KYCVPFHCVILRDKV"
     gene            3557..3751
                     /locus_tag="VN039_06355"
     CDS             3557..3751
                     /locus_tag="VN039_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45628.1"
                     /translation="MNQHMTITRKHVNKYANQDSCFRHGIGISKCRVCLKSDKVGDLS
                     RGAKHRKMMETLTGYPRMGL"
     gene            3826..>4027
                     /locus_tag="VN039_06360"
     CDS             3826..>4027
                     /locus_tag="VN039_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45629.1"
                     /translation="MSDYQNEAGEWLMDGAAMRFEQQLDMEAQADYYADTFYEDAYDE
                     QPDDGYDDEPEFYQESEDFWLDG"
BASE COUNT          913 a         1167 c         1019 g          928 t
ORIGIN      
        1 atacgcccta tagcaattcg aacacctgtt cgatagtgcg cacatccata ccaatgtgac
       61 cacatggggg aatcgaacgt ctgttcgata caggggatat gcgtctcatg aggtagacgg
      121 tactaccctc cccagacatg cgcctagcct gccatgctca cacactgtca ggcttgcgag
      181 aatcgcttgt aagcctcccg ataccctccc ccgggggaat cccccggggg atgcttccta
      241 gcccgttaaa agccatccta ggaagcctag gcgtatcgtg aacgcgtctc cactgtcggc
      301 cgacgcgacc accatgcccg agcatccgcc acactcggca cacgcacgcc ccgggatgcg
      361 ttggccacat ctagcgcgta tcgggatgcg tgtgtccagt cgcgtcccac tgattgcaat
      421 atgcctcgcc cctgattgca tccacggcac agataaacga cattcccagg gcggtagtca
      481 ttagcgggaa tgcacctatc tacctcggcc gtatctaggt cgagtgacgc cgaacatgcc
      541 ggacattcta aacgaccagc cgttaagacc atcgtgacaa gataagcgta atagttgcgt
      601 tgaatacgcc tagcgcttcc ccatgcctta gatgacttag accatatggc aattgtggaa
      661 ggtgcacccg caataggtgc accttccatg gcagacagtc tccccatggc tcattcacca
      721 atctgaatcg agcggataag ccagacattg aaactacgcg gtcccttgtc agtatcgact
      781 agaacggttt cggtagacat gccgtagtcg ccactgaatc cgataaccgt accggtccga
      841 taggtgaccg tgcccgagtc cctaccctgt actgagaagg aaacacccct gcccgcacta
      901 tcggcagtct tgagaacatc ccgcatacgc tcggcatgcg cggcagtctc agagtcaagt
      961 gcaaacgtat tgtgtcgtgc catgatgcta cttcctatct tgtgtgctcc cccgggggat
     1021 tgttcccccg ggggatattg ggctgaacta ttcagaatct tccaattccc aatcggcttg
     1081 ttccacgttc ttgtaatggt cgtattcttg cgaccacaat tccgccggaa tctcgctcat
     1141 gctttaacct ccactagttt gccgcaatcg ttgcattccc agaccttgaa gaaaacacct
     1201 gccttgtcgg catactcgtg cagcctgcca tcgtcgcaga cacaatcggc gatatccgac
     1261 tcgtgaataa cttccgggtc gagtgactcc ggagtctccc atgcagtgtc gtgctgccat
     1321 tgtgcgagtt catccattgt ttcgtcggta aggtacattg tcatgccttc cattgttgtt
     1381 tgtttttctg gattcttttc ccgtaccgtc tcggtatatt cacgacgtta gcgaacctaa
     1441 cgcttactta cctagattcc gtctactcgt taatctcacg gattaggctc cgatatcact
     1501 ccgggtccct attgagttgt gtggtgcagc acgaacgtta ccctacctat gggagcgtgg
     1561 gcacgttttt gccaactcca ccgcacaccc gtatggtgaa tccgtgcgtt tgcgcaggtc
     1621 acaggcttgt gaaagttttt tcgattccca tactgggatg atggacaggt tcggcgattc
     1681 agccacgttt ccggccgatt ctggcacgct cggaccacag acatatgcgt cggatatcgg
     1741 catattgaat agccgttcaa tggaccagca gcccaacaaa ctaaagtcaa tccgtctact
     1801 tggctttggc ggcacatctc acatgtgaga tgtccagcat gtgaaacgtc tgtccagcat
     1861 gtggacaacg gcgtcgttcg aacacctgtt cgaacgaccc ccgggggttg gcgggtcgga
     1921 aaaccctttt acgaccaggg gcgaaatcgc agggattctc acgcgcgatt acttaaaacc
     1981 cggggatttt tcactagaat gcttgacaga ttgccagtaa tggcctagat tgtactcatg
     2041 accaagaaac tgagttcccg ttccatcgct gttgccgagg ccatccgtga ccgtgagcca
     2101 ttcgagacat acgggactct cagcgctacc cgccacatgg gctacgccca accgggtcgc
     2161 ctgtcgggtc ccgaccttga cgccttcaat cgtgaccaag gcagtttttc gtaaggaaaa
     2221 atcatggaac agaaagtttg gtactacaac tcgatacttc gcatacttcg acggtcaaat
     2281 tgcaccgaag aaagggctgc ggaactcatg aatatccaca cgcttcctta cttgatttac
     2341 ttcgtcgaag aggtctaaga agccctcatg tcaaccctgg gtatgagcca gggttgaccc
     2401 ccggggggca tattcgcaat attcattacg atagatgctt gacatcgggg aactttaggc
     2461 ttatctttaa tacatgacct tcacgcctgc aaacctcgtc gccaaccgta ccgctcgcct
     2521 cgtggccgag gccaccacgc ccgtctactc gaccgacacg gctggcgcgg cagccaagga
     2581 gcgtctgcgc aagttagagt gggccgagga gttcacgcac agcgaggacg accgccagcc
     2641 ttggtgtcac gtgtgcagtc gtccgacaga ccacttcggt gagcacagcg acgagcaatt
     2701 gctcgcattc tacaacggcc agggaaggaa gttccgacga tgagcgaacg agtctggcta
     2761 gtcatctacg cccttgtgat tgcggccttc gcaatctata tcctcttcta cgctgcctca
     2821 gtctgaggca gcacccccgg ggggcggtaa cgacaagatg attaagttaa gtccaaccag
     2881 aatttcgact gaaaaatcga ccagtttctt gtttcgacca gaaaatcgtg ttaggattgg
     2941 ctcatgacca gaaaacacta catcgctacc gctgccatgt tcgctgacat gatggatggc
     3001 atgtccatca taggctcgaa cttcattgcc gggtacgccc gagccattgt cgatgctgcc
     3061 aacaacatgg cagacatctt cgaggcagac aatcctcgtt tcgaccgtga caagttcatc
     3121 aaggcgtgcg ggctctgaac ccgcacgacc cccggggggc gggtattgac aggcattaag
     3181 atgtacggta cgattcttcc atgaagactc tcattctcgc aatcgttatg gtgttcgccc
     3241 tggcctgccc ggcgtcagcc gaccagacgc caggagtggt cacggttcac gagttcaatc
     3301 atgcccagaa gggatggact cgcgcgcagg tcaccaagtc ttttggcaca cacggccatg
     3361 ccatcggagg caattggatt cagggacgat tcatcctgac cgtcaagtat cacggcatca
     3421 atggccccac tttcgccaat tttgtcgacc gtggcaccac tggcgacaat ttccaccttc
     3481 ggaatcttta ccgattcacc aaaacaaagt actgcgttcc cttccattgt gttatcttgc
     3541 gtgataaggt gtagccatga accagcacat gaccatcact cgcaagcacg tcaacaagta
     3601 tgccaaccag gacagttgtt ttcggcatgg catcggcatc tccaagtgtc gtgtctgttt
     3661 gaagtctgac aaggttggcg acctctcgcg tggtgccaag catcggaaga tgatggaaac
     3721 tctcaccggc tacccgcgca tgggcctcta gcccgtcgcc cttcggggcg acccccgggg
     3781 ggcaaaacac ttgacaacct gactgtttac aattactatt gctctatgag cgattaccag
     3841 aacgaggccg gtgagtggct gatggatggc gcagccatgc gtttcgagca gcagttggac
     3901 atggaggctc aggctgacta ctatgccgac accttctacg aggacgccta cgacgagcag
     3961 cccgacgacg gctacgacga tgagcccgag ttctaccagg agtctgagga cttctggctg
     4021 gacggct
//
LOCUS       DATMTI010000387         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_524331, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000387 DATMTI010000000
VERSION     DATMTI010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524331"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VN039_06365"
     CDS             <1..426
                     /locus_tag="VN039_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWV45630.1"
                     /translation="GSMGNAPAIALGVALAKPNKQVVTFDGDGNVLMGMGTLATVGAL
                     KPKNFIHVVFDNEVYGTTGNQPTISNVVPLEKVAKSAGYVNVERVLDRDDLVYEFKDM
                     LKKDGPSMLLIKVNEFVEDAGRVLHDPPDITRRFMKAIA"
     gene            528..1610
                     /locus_tag="VN039_06370"
     CDS             528..1610
                     /locus_tag="VN039_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWV45631.1"
                     /translation="MILLNPGPVNVSERVRQALLKPDICHREDEFSELLHRIQAKLLK
                     AFVPGAESEYVAVVMTGSGTAAVEAALMSSLPHGRRMLILNNGVYGERMSQIVGLNRL
                     GVSELKYEWTVRPDPEKLLLALRQHQEVHAVGMVHHETTTGLLNPVHEIAEVVDSQNR
                     VFVLDAVSALAGETIDIGRSHIYMVAGTAGKCIQGFPGVSFVLVRKGFVEKMRSYPKR
                     SWYLHLTHYIDNEGRGTIPFTPAVQVYYAFDEALNELLEEGVTTRIQRYKKMATLIRE
                     RMAKLGVKALLPVDRQSNTITAYHLPNGVSYHALHDRLKQQGYVIYAGQGNLENKIFR
                     VANMGALSKAQFDGFLDAFEQVCKST"
     gene            1607..2326
                     /locus_tag="VN039_06375"
     CDS             1607..2326
                     /locus_tag="VN039_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HWV45632.1"
                     /translation="MKAVILAAGVGKRLWEVTQHRPKCLIEIGGQSLLHRYLESLASV
                     GIRRAEIVVGYKQEMIRAAVEQDACGVNVKFLVNEQFHRGSISSLWIARTALDDDTIV
                     MDADVLFHQEILRRLVSSPAGNALLMDETVKQTGEECMVVVAGERVVALTKNMPEQYD
                     YAGEGVGFLRVRHADTPRVVSSLRGYIDRGSWDMEYEDALRQYFQDVRVGYERIGGLP
                     WTEIDFIEDVKKAELEVLPRL"
     gene            2414..>3456
                     /locus_tag="VN039_06380"
     CDS             2414..>3456
                     /locus_tag="VN039_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWV45633.1"
                     /translation="MSKSILQRRAEIQGLATAILLPSVGIFGDPIGSQVGDVGPLTSV
                     VGIGLFQRTVLTLQRAGIRQLIVLSGSEEEQLKQVLGKGPRVTIPVRWMPVREFPLDD
                     PRTWEALAAEVHGFALVASVNSVFSRGLIERLRRDVQDGQAIVVSQTPQRQVDQLVLR
                     DVRSKVLSGRSAIYTAPRFDDPALRVAELVVVPASLMNTPNQSASEKGSPPIRQWLER
                     AAADGRVHVVSAEERRGNWYQPVRTLADVQTAEKKLFNSLKGEFEGFVDRYFNRKVSR
                     WFTRLFLAIGLSPNSITVLAGLIGLVAAVGFGMGTYGAGIVAALLFQLAAVIDCCDGE
                     VARLTFTESPFGA"
BASE COUNT          746 a          897 c         1060 g          753 t
ORIGIN      
        1 ggctccatgg ggaatgcccc ggcgattgca ctcggtgtcg cactcgcaaa gccgaataag
       61 caggtggtta ccttcgatgg cgacgggaat gtattgatgg gaatggggac actcgcaacg
      121 gtcggcgcgt tgaaaccgaa aaactttatt cacgtcgtat tcgacaacga agtgtacgga
      181 acgaccggga accagcccac gatctccaac gtcgtgccgc tggagaaagt ggcgaagtcg
      241 gcgggctacg tgaatgtgga acgggtactt gatcgcgacg atcttgtcta cgaattcaaa
      301 gacatgctga agaaggatgg gcccagcatg ttgctcatca aggtgaacga atttgtggaa
      361 gatgccggcc gcgtcttaca cgatccgccg gatatcaccc gccggttcat gaaagcgatt
      421 gcgtaggact gagcaatgag gactgagggc cgaaaaggaa agatgcgaat cacggcagcg
      481 ccgctcttca gtcctcagtc ctcagtcctc agtcctggag ctagcgcatg attctcctca
      541 atcctgggcc ggtcaacgtt tccgagcggg tacggcaggc gctgctgaaa cccgatatct
      601 gccatcgaga agatgaattt tccgaactgc ttcaccgcat ccaagccaag ctgctcaagg
      661 cctttgtccc cggagctgag tcggaatacg tcgccgtcgt gatgactgga tcaggcacgg
      721 ctgccgtcga agctgccttg atgtcgtcgc tccctcacgg ccgccgtatg ctcatcctca
      781 acaacggcgt ctatggtgaa cggatgtctc aaatcgtggg cctcaatcgc ctgggggtaa
      841 gcgagttgaa atatgaatgg acggttcggc ccgatccgga aaaattgctt cttgccttgc
      901 gacagcatca ggaggtgcat gccgtcggca tggtccacca tgagaccacc accggactcc
      961 tcaatcctgt ccacgagatc gccgaggtcg tcgacagtca gaatcgggtg tttgtgctcg
     1021 atgctgtcag cgctctggcg ggcgagacga tcgacattgg gcggtcgcac atttatatgg
     1081 tggcggggac ggcgggcaag tgtattcagg ggttcccagg cgtctcgttc gtactggtgc
     1141 gaaaggggtt cgtcgagaaa atgcgctcgt acccgaagcg ctcgtggtat ctccatctga
     1201 cccattacat cgacaacgag gggcgaggca cgattccctt cacgcctgcc gtgcaggtct
     1261 actatgcctt cgacgaagca ctcaacgaac tactcgaaga aggcgtgacc actcgcatcc
     1321 aacggtacaa gaagatggcg accctgatcc gtgagcgaat ggccaagctc ggtgtgaagg
     1381 cgctcctccc ggtggaccgg caatcgaaca ctatcacggc ttatcacttg ccgaatggtg
     1441 tttcctacca cgcgttgcat gatcgtctga agcaacaagg ctatgtcatt tatgccggtc
     1501 aaggcaactt ggaaaacaag attttccgcg tggccaatat gggagcattg tccaaggcac
     1561 aattcgacgg gtttctggac gccttcgagc aagtgtgtaa atcgacatga aggcggtcat
     1621 ccttgcagcg ggagtgggaa aacgtctgtg ggaagtgacc caacatcgtc caaaatgcct
     1681 catcgagatc ggaggccagt cgctcctaca tcgctactta gagtcactgg cttccgtcgg
     1741 gattcgccgg gcggagatcg tcgtcgggta caagcaggag atgattcgtg cggcggtcga
     1801 gcaggatgcg tgcggcgtca acgttaaatt cctggtcaac gagcagtttc acagaggcag
     1861 tatttcctct ctatggatcg cacgaaccgc gctcgacgac gatacgatcg tgatggatgc
     1921 cgacgtgctg ttccatcagg aaatcctacg gcgtctggtg tcgtcacccg ctgggaatgc
     1981 gctgttgatg gacgagacgg tgaagcagac cggagaagaa tgcatggtgg tcgtggcggg
     2041 ggagagagtt gtcgcgctga caaagaacat gccggaacag tacgactacg ccggtgaagg
     2101 ggttggattt cttagagttc ggcatgccga cacacctcgc gtcgtctcct ccctccgtgg
     2161 ctacatcgac agaggctcgt gggatatgga gtacgaagac gcgctgcggc agtattttca
     2221 agacgtccgg gtcggttatg aacggatcgg aggactgccc tggaccgaga tcgattttat
     2281 cgaagatgta aaaaaagctg aattggaagt tttgccaaga ttatgagaaa gtcttcacca
     2341 tggtagaatt tccgatgggg gaaggccctg cgcacggaat caccggcaag cagacgcggt
     2401 tgtgtatgga accatgagta agagcatcct tcaaagaaga gcagaaatcc aaggactggc
     2461 gacggcgatt ctgctgccgt ctgtcgggat attcggcgat ccgatcggat cgcaggttgg
     2521 cgacgtggga cctctgacga gcgtcgtggg gatcgggctc tttcaacgga ccgtgcttac
     2581 cttgcaacgg gcggggatcc gtcaattaat cgtcctttcc ggttctgaag aggagcagtt
     2641 gaaacaggtg ctcggcaaag gaccgcgcgt cacgattccc gtccgatgga tgccggtccg
     2701 tgagtttcct ctcgacgatc ctcggacatg ggaagcgttg gcggccgaag tgcatggctt
     2761 tgcgttggtc gcgagcgtca atagtgtgtt ctctcgaggg ttgatcgaac ggttgcggcg
     2821 tgatgtacag gacggtcagg ccatcgtcgt ctctcaaacc ccacagagac aggtcgatca
     2881 gctcgtgttg cgtgacgtga gatcgaaggt cctgtccggt cgctccgcga tctacaccgc
     2941 tcctcgtttc gatgatccgg ccttgcgtgt cgccgagctg gtggtcgtgc cggcgagtct
     3001 gatgaacacc cccaatcaat cggcgagtga gaagggaagc cctccgatcc gacagtggct
     3061 tgagcgggcc gctgcggatg gtcgagtgca tgtcgtctcg gctgaagaga ggcggggtaa
     3121 ttggtatcaa ccagtccgaa ccctggctga cgtccaaaca gccgaaaaga agttgttcaa
     3181 ttcgctcaaa ggggaatttg aagggttcgt cgatcgctat ttcaatcgca aagtctctcg
     3241 ctggttcaca cgcctgtttc tcgccatagg gctgtcgccc aattccatca cggtccttgc
     3301 cggattaatc ggtctggttg ctgcggtggg gtttgggatg ggcacgtacg gtgccggtat
     3361 tgtggcggcc ctcttgtttc aattggcagc cgtgattgat tgttgcgacg gcgaagtcgc
     3421 gcggttgacc tttaccgagt cgccctttgg tgcctg
//
LOCUS       DATMTI010000388         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_438922, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000388 DATMTI010000000
VERSION     DATMTI010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438922"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VN039_06385"
     CDS             complement(<1..276)
                     /locus_tag="VN039_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45634.1"
                     /translation="MSDERGRAMNRHPTQLVSTIVGWGLILGLAGCDYWPPALQAEIE
                     QLRSETQTLTMEKTQLQAQVTDLSKTKQELQGQIDELSYINRGKTEMI"
     gene            complement(497..1771)
                     /gene="purD"
                     /locus_tag="VN039_06390"
     CDS             complement(497..1771)
                     /gene="purD"
                     /locus_tag="VN039_06390"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWV45635.1"
                     /translation="MKILVVGSGGREHAMVWKLAQSPRKPLLYCAPGNAGIASLAECI
                     PIKSDDVAGLKDFALREKVDVTVIGPEAPLALGIVDEFRRARLRVFGPTKNAARLEAS
                     KIFSKEVMARAKIRTAQAKSFEKIADALAYVERQELPIVIKADGLAQGKGVIIATARQ
                     QAQQAIVDSMEKAVFGQAGKQVLIEEFLDGEELTIMAFTDGRTVVPMPPAQDHKRVGD
                     GDTGLNTGGMGAYCPAPLGTPELQDRVRREVLQPMVDAMARLGSPFQGVLYAGLMVVN
                     GMPYVLEFNARMGDPETQVVLPLLKTDFLDVIESVVEHRLDQLPVEWHPDATVCVVMA
                     SGGYPGSYRQGMPLSGLPPATISSDSSMVFHAGTAQKGNEIVTAGGRVLGILGRGRTL
                     SEAQREAYRAVKTISFEGCHFRTDIAHRALKT"
     gene            complement(1923..3476)
                     /gene="purH"
                     /locus_tag="VN039_06395"
     CDS             complement(1923..3476)
                     /gene="purH"
                     /locus_tag="VN039_06395"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWV45636.1"
                     /translation="MAGIKRALISVSDKTGVIEMAKALEALGAEILSTGGTAKALRDA
                     GVKVTDVAAYTGSPEILDGRVKTLHPKIHGGLLGRRSLPAHVDQMNQHGIGPIDVVVV
                     NLYPFEATIAKPDCRFDEAIENIDIGGPSMLRSAAKNHDDVLVVVDPADYSRVLEAVT
                     GNIVTPALRRELAMKVFQHTSRYDGLIAGYLEKHANGGEIKFPKVLSLQFELAESLRY
                     GENPHQQGAFYRELDSKEPSVSRGKILHGKAMSYNNFLDANSALELIKEYGETAVAII
                     KHNNPCGVALGETPTEAYVKARETDPVSAFGGVIAFNRPVDMTAAKEITSTFVEVVIA
                     PGFAEDALAELKRKKDLRLLDVGPLMKVKQEGFDLKKLVGGLIVQDRDLGVLSDLRTL
                     AVPTIRKPTDEEYAACAFAWKVCKHVKSNAIIYAKPGQTVGIGAGQMSRVDSVKLAAM
                     KAQMPVKGCVMASDAFFPFRDGLDAAAEVGITAVIQPGGSIRDAEVVKAADEHGMAMI
                     LTGMRHFRH"
BASE COUNT          765 a         1062 c          921 g          804 t
ORIGIN      
        1 aatcatttcg gtttttcccc ggttgatgta gctcagttca tcgatctgtc cctgtagctc
       61 ctgtttggtc ttggacaagt cggtgacctg tgcctgaagc tgagtcttct ccatggtcaa
      121 ggtttgggtc tctgaccgca attgttcgat ctcagcttgc aaggccggcg gccaataatc
      181 acatcccgca aggccaagta tgaggcccca accaactatc gtactgacta actgcgtcgg
      241 gtggcggttc atggcccttc ccctttcatc agacatgatg gttccacacc agtgtttgta
      301 tgggcttcct gtgtcttcgc catgtgggta cactgaccgt gatggaaaag tattctaaca
      361 gatcgcacga atcggctaaa aagaagatcg gccccggcta ttcggatcta ggcctgcttc
      421 tgtaggtaga tctatctcag cagcagacgg gatatgatcg acatcattgt cgaatcggtg
      481 gctcgaggat ggctgactat gtcttcagag cccggtgcgc aatgtccgtc cggaagtggc
      541 aaccctcaaa ggagatcgtc ttcacggctc gataggcttc gcgttgtgcc tcggataacg
      601 tgcgtcctcg accgagaatg ccgaggactc gaccgccggc tgtcacgatc tcattgccct
      661 tctgtgcagt tcccgcatga aagaccatcg aggagtccga cgaaatggtt gctggaggga
      721 gtccagaaag cggcatcccc tgtcgatagg agccgggata gcctcccgat gccatcacca
      781 cgcagaccgt cgcgtctgga tgccactcga cgggaagttg atctagccga tgttcgacca
      841 ccgattcgat gacgtcgaga aaatcggtct tgagcaaggg cagcaccact tgtgtttctg
      901 gatctcccat acgagcgttg aattcgagca cgtatggcat tccattcacc accatcagtc
      961 ctgcatatag gacaccttgg aatggtgaac cgagccgtgc catggcatct accatcggct
     1021 gtaagacctc gcgacggacc cgatcttgga gctcgggtgt tcccaacggt gccgggcaat
     1081 aagcgcccat tccaccggta ttcaagcctg tgtcgccatc accgacccgc ttatgatcct
     1141 gcgccggcgg catcggcacc acggtcctgc catcggtaaa ggccatgatc gtcagctcct
     1201 cgccgtcaag gaactcctca atcaacacct gctttccggc ttgaccaaac acggcctttt
     1261 ccatcgaatc cacgatcgcc tgctgtgctt gctgacgagc ggtggcaatg atgacgcctt
     1321 ttccctgagc cagcccgtcc gctttgatca cgatcggcaa ctcctgccgt tcgacatagg
     1381 ccagggcgtc agcgatcttt tcgaaactct tggcttgagc ggtccggatc tttgctcgtg
     1441 ccatgacttc cttcgagaag atcttgctgg cttccaaacg agcagcgttt ttggtcggcc
     1501 caaacactct gagccgagct ctgcggaatt cgtcaacgat gcccaacgcg agaggcgctt
     1561 caggcccgat caccgtcaca tcaacctttt ctcgaagagc gaagtctttc agaccggcca
     1621 cgtcgtccga cttaattggg atgcattccg ccagtgacgc aatgcctgca ttgccggggg
     1681 cacaatacag taggggcttg cgtggactct gcgcgagctt ccacaccatc gcatgctcgc
     1741 gtcccccact gccgaccacg agaattttca tagattttct tcaggtagga aagacaaaga
     1801 ggctgcccaa aatggcctgt cgcaaggccg caggaagctc ggacaccgag gcgtacgttt
     1861 tccggtacgt tgaggtggac gggcgaccga gaacgcagcg agaggccatt ttcagctgcc
     1921 gatcaatggc ggaaatgccg catgcctgtg agaatcatcg ccatcccatg ttcatccgcc
     1981 gctttgacga cttctgcatc ccggattgat ccgcctggtt gaatgacggc cgtaataccg
     2041 acttcggctg cggcatcgag gccatcgcgg aacggaaaga aggcgtccga agccatgaca
     2101 caacctttga ccggcatttg cgctttcatg gccgccaact tcacggaatc cacacggctc
     2161 atctgccctg ccccgatgcc gacggtctgg ccgggcttgg cataaatgat ggcgttggac
     2221 ttaacgtgtt tgcagacttt ccacgcaaac gcacaagcag cgtattcttc atccgtcggc
     2281 ttgcgaatgg tcggcacggc aagcgtccga agatccgata agacaccgag atcgcgatcc
     2341 tgtacgatga gcccgccgac aagtttcttc agatcgaacc cttcctgctt caccttcatc
     2401 aaggggccca cgtccaataa acgcagatcc ttcttccgct tcagttcggc taatgcatct
     2461 tcagcaaacc ctggagcgat cacgacctca acaaacgtgg acgtgatttc cttggcagcc
     2521 gtcatatcca ccggccggtt gaaggcaatc acaccgccga aggcggacac gggatccgtc
     2581 tctctcgcct tgacgtacgc ctccgtgggc gtttcaccga gcgccacccc acagggattg
     2641 ttgtgtttga tgatcgcaac ggccgtctct ccatattctt ttatcagctc gagcgcggag
     2701 ttcgcatcga ggaagttatt gtaagacatc gctttgccgt gcaagatctt cccacgagaa
     2761 accgatggct ccttgctgtc caattcacga tagaacgcgc cttgctggtg aggattctct
     2821 ccgtagcgga gggattccgc caactcaaac tgaagcgaca atactttcgg gaacttgatc
     2881 tccccgccgt tcgcatgctt ctccaaatag cccgcgatca atccgtcata acgcgacgta
     2941 tgctgaaaaa ccttcatcgc caattcacgg cgcagcgccg gtgtcactat attgccggtc
     3001 acggcttcca gcacccgcga gtaatcggcc ggatcgacta cgaccaacac atcgtcatga
     3061 ttcttggccg ccgaccgcag catggacgga ccaccgatat cgatattttc aatggcctca
     3121 tcgaaacggc agtcgggttt ggcgatcgtg gcttcaaagg gatagaggtt gaccacgact
     3181 acatcgattg gaccgatccc atgttgattc atctgatcga catgcgccgg aagcgaccgg
     3241 cgccccagca agccgccatg aattttgggg tgtaacgttt tcactcgacc gtcgagaatc
     3301 tccggcgatc ccgtataggc ggcgacatcc gtgactttca cccccgcatc gcgcaacgct
     3361 ttagccgttc ctcccgtgga caaaatttcc gcgccaagcg cttcaagtgc cttcgccatc
     3421 tcgatgactc cggtcttatc cgaaacactg atcaacgccc gcttaatgcc ggccatgtga
     3481 gtactcctta ggattgtgtg cgatgtggaa gagatacgtc gactacgaaa ggcatgcgaa
     3541 gaatagcaaa tg
//
LOCUS       DATMTI010000389         1815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_524376, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000389 DATMTI010000000
VERSION     DATMTI010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524376"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN039_06400"
     CDS             <1..322
                     /locus_tag="VN039_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45637.1"
                     /translation="APWDRYNGFVVDTDASLRLASLQAWLRHGSLDGDLLTWIAKAGQ
                     NFYDPYTGLPMLVNLKKGLLYSVGHDGKDQDADPLSDVVVEIPAVQTSAAQAKSSAVS
                     SKSR"
     gene            423..>1815
                     /locus_tag="VN039_06405"
     CDS             423..>1815
                     /locus_tag="VN039_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV45638.1"
                     /translation="MGHSPRPFFEDLSVRKGATFPTAVPTQIVSNEEFFPICQTAEQV
                     CLERVTRELVEKGAAQRGLTRRDFLKTTGGTAAALLAMNDAFGRFFNIGEIELFETAA
                     FAEQQGDPYFIFDAQTHYVSSHYDPSEAESGRKGAVSKQALLSLRKHIREAGLNPKLA
                     EDRGTLDDLSWKNFVKEVFFDSETTVGLISTPPGPYPQEAVVPPKEMAHIRDEINRLA
                     GSQRMLAHGLATPQLGAADLEFMAMQAETLKVDAWKCYTGSCPKGFDRGWRMDDERLA
                     YPMLEQARKLNIKRICVHKGLPLGPVPDYSHPRDLIKAAKDFPDLNFLVYHSGFRGAA
                     SIDQIFAKTGEIPWTTEFCRMKEAEPSISNIYMELGSTFAQLVTTYPLICAHLLGQII
                     RSFGVDHVLWGTDSIWYGTPQWQIEAFRRFQIPEELIEKHQYQPLTRRVKERVFGLNS
                     AGVFGVDVEAKRRE"
BASE COUNT          416 a          479 c          527 g          393 t
ORIGIN      
        1 tgctccatgg gaccggtaca acggatttgt ggtcgatacg gatgcctctc tccggcttgc
       61 cagcttgcaa gcctggctca gacatggttc cctcgatgga gatctcctca catggatcgc
      121 gaaagcaggg caaaatttct atgaccccta tacgggactg ccgatgttgg tgaacttgaa
      181 aaaggggctc ctctacagtg ttggacacga cgggaaagat caagatgccg accctctgtc
      241 ggatgtggtg gtagagattc ctgccgtaca aacgtctgcc gctcaggcaa aatcatcagc
      301 cgtttcatcc aagtccagat aagcctttcc ccgctacttc aggcctgctc cggcatttgc
      361 ttgacagccg cttggagtat ttcacacaat cagtgagctt tgcatagatg aagaggtctg
      421 tcatgggaca ctcaccaaga ccattctttg aggatctctc cgtgaggaag ggtgcgacgt
      481 ttccaaccgc tgtgccgacg cagatcgtat ccaacgagga gtttttcccg atttgccaaa
      541 cagccgaaca ggtttgcctc gagcgggtaa caagagaatt ggtggaaaag ggagcagctc
      601 aacgtggatt gacgcgtcga gacttcctaa agaccaccgg cggtacggcg gctgcgttgc
      661 tggccatgaa cgacgcgttc ggtcgatttt tcaacatcgg agaaatcgag ctgttcgaaa
      721 cagcggcgtt tgccgagcag cagggagatc cgtattttat cttcgatgcc caaacccact
      781 acgtcagttc ccactacgat ccctctgagg ccgaatccgg tcggaagggc gccgtttcca
      841 aacaggcact gctgtcgctg agaaagcaca ttcgtgaggc gggactcaac ccgaagctcg
      901 cagaggatcg aggcactctc gatgatctct cgtggaagaa ttttgtaaaa gaagtgtttt
      961 tcgacagcga gaccaccgtc ggcttgatca gcacgccgcc cgggccctat ccgcaagaag
     1021 ccgtcgtccc gccgaaggag atggcccaca tccgcgatga gatcaatcgg ttggccggtt
     1081 cgcaacggat gctggctcat ggtctggcga cgccgcagct gggagcggca gatttggaat
     1141 tcatggcgat gcaggccgaa actctcaagg tcgacgcctg gaaatgctac accggttcct
     1201 gcccgaaggg gttcgaccgg ggctggcgga tggatgatga gcgcctcgcc tatccgatgt
     1261 tggaacaggc gcgaaagctc aatataaaac ggatatgcgt tcacaaggga ctgcccctcg
     1321 ggcctgtgcc ggactacagc catcccagag atctgatcaa ggcggccaag gacttcccgg
     1381 atctcaactt tttggtgtat cactccgggt tccgaggcgc ggcatcgatc gatcagatat
     1441 ttgcgaagac cggcgagatt ccgtggacca ccgagttctg ccggatgaag gaagcggagc
     1501 cgagtatctc caacatttat atggagttgg gttcgacctt cgcgcaattg gtgacgacct
     1561 atccgttgat ctgcgcgcat ctgctggggc agattatccg ctcgttcggc gttgatcacg
     1621 tactgtgggg gacagactcc atctggtatg gaacgccgca atggcagatc gaagcgtttc
     1681 ggcgattcca aattcccgag gaattgatcg agaaacatca gtatcagcct ctgacgagac
     1741 gggtgaagga acgggttttt ggattaaact cggccggagt gtttggagtc gatgtcgaag
     1801 caaaacgacg cgagg
//
LOCUS       DATMTI010000390         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_15162, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000390 DATMTI010000000
VERSION     DATMTI010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15162"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VN039_06410"
     CDS             complement(<1..110)
                     /locus_tag="VN039_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWV45639.1"
                     /translation="MATGELLYEGKAKKIFLTGNPDEVVQYFKDDATAFNA"
     gene            complement(133..930)
                     /locus_tag="VN039_06415"
     CDS             complement(133..930)
                     /locus_tag="VN039_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HWV45640.1"
                     /translation="MTRSLVTGLMLIGFMVGPWASNTQAATDREKLLTEPGVYGTFAV
                     FALNNEEWVRQDPATRIVRLTSLKGVVEQHREHVAIDLYLLRGLSDHGDLLFRIHAAE
                     LRETQEFLVDLKSSQFGKHLKTVGIMHGLTKKPTYVPGFPDQMKDDLRTISDPGQKPY
                     AVVIPIRKSAEWWGLDQEKRAAMMQEHTAATLPYLKTVKRKLYHSSGLDDSDFITYFE
                     TSKLEDFHSLVQSLEKVQEFRYTRRFGHPIILGTVKSLDEIVELLAQ"
     gene            complement(927..2267)
                     /gene="purB"
                     /locus_tag="VN039_06420"
     CDS             complement(927..2267)
                     /gene="purB"
                     /locus_tag="VN039_06420"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWV45641.1"
                     /translation="MIERYTRPRMKAIWDLKHKYEIWLEVELQACAAFEQAGQAPRGT
                     AAKIRKKAKIDVERIAEIEKITKHDVIAFLESLMDTVGPEHRFLHMGLTSSDIVDTSL
                     AIQMTEALDLILGGVDELLVVLKQQAFRYKNQVMVGRSHGIHGEPISFGLKMALWYEE
                     VRRHLERLRHVRSEIAVGKLSGAMGTFAHQGPDIEDYVCAKLGLKIDPVSNQVVQRDR
                     HASYSTALALLAASIEKFATEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPIVSEN
                     LCGLARVVRANSIAAMENVALWHERDISHSSVERVIMPDSTILIDYMLAKVTDLIKHL
                     VVYPDRMRQNLELTGGLIYSQRLLLTLVEKGAQRKESYEAVQRNAMASWRGGGGLQEL
                     AGKDPFISEHLTKSEIAACFNPTHYLRHLDQIYRRVFGRDRQRLPGIRAKGVRS"
     gene            complement(2260..>2461)
                     /locus_tag="VN039_06425"
     CDS             complement(2260..>2461)
                     /locus_tag="VN039_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWV45642.1"
                     /translation="YELLWLDEVPAKVTMDEAIELAKSFGDNDASKFVNAVLDKALAT
                     ELRLAAKRADPDRGIWRRADSD"
BASE COUNT          514 a          743 c          608 g          596 t
ORIGIN      
        1 gcgttgaagg cggtcgcatc gtccttgaaa tactgcacga cctcatccgg atttcccgtc
       61 aaaaaaatct tcttcgcttt gccttcgtaa agaagctcac ccgtcgccat gatcggacct
      121 caatggttcg gcttactgcg ccaacagttc tacaatctcg tccaatgatt tcactgtacc
      181 gagtatgatc ggatgtccga accgccgcgt atagcggaat tcctgcactt tttcgagcga
      241 ctgcaccagg ctgtgaaagt cctccagctt ggaggtttca aaataggtaa tgaagtccga
      301 atcgtccagt ccgctggagt gatagagctt ccgtttgacc gtctttaagt aaggcagcgt
      361 ggcagcggta tgttcttgca tcatcgcagc ccgtttctcc tgatccagcc cccaccactc
      421 ggccgatttc cggatcggaa tcacgacggc atatggtttc tggccggggt cactgatggt
      481 gcgtaagtca tccttcatct gatccggaaa tcccggcaca taagtcggtt ttttggtgag
      541 cccgtgcatg atcccgaccg ttttgaggtg tttcccgaat tggctgctct ttagatccac
      601 gagaaactcc tgcgtctcgc gcaattctgc tgcatgaatc ctgaacaaga ggtcgccgtg
      661 atcggaaagc cctcgaagga ggtagagatc gattgccacg tgttctcgat gctgttccac
      721 cacacctttc agcgacgtca gacggacgat ccgcgtcgct gggtcttgcc tcacccactc
      781 ctcattattg agcgcgaaga cggcaaacgt gccgtagacc cccggttcag tgagaagctt
      841 ctctcgatcc gttgccgcct gagtgttcga tgcccaagga ccaaccatga acccaataag
      901 catcagcccc gtgacgagtg atcgagtcat gatctcactc ccttcgccct gatgccgggc
      961 aaacgttgcc tgtcgcgtcc gaacacccgc cgatagatct gatcgaggtg gcgcagatag
     1021 tgtgttgggt tgaaacaggc ggcaatttca ctctttgtga gatgctctga aataaaagga
     1081 tctttgccgg ccaattcttg taatccacct cctcccctcc aggaggccat ggcattgcgc
     1141 tgaaccgctt cgtatgactc ttttcgttgc gcccccttct caaccagcgt caacagcaac
     1201 cgctgcgaat aaatgagtcc gcccgtcagt tcgagattct gtctcatccg atccgggtag
     1261 acgaccaagt gcttgatcag atccgtcacc ttggcaagca tgtagtcgat caaaattgtg
     1321 ctgtccggca tgatgacccg ctcgacggac gaatggctga tgtcgcgttc atgccacagt
     1381 gcgacgtttt ccatcgccgc tatgctgttg gcccgcacca cccgagccaa gccgcagagg
     1441 ttctcggaga caatcgggtt ccgtttgtga ggcatcgccg acgatccttt ttgtccttcg
     1501 gagaagaact cttccgcttc gagcacttca gtacgctgaa gatgacgaat ctcggtggcg
     1561 aacttctcga tgcttgcggc aagcaaggcg agcgctgttg aataggacgc atggcgatcg
     1621 cgttggacta cctgattaga gactggatcg atcttcaatc caagcttggc acagacatag
     1681 tcttcaatgt ccggcccctg gtgcgcgaag gtccccatcg cgccggacaa cttgccgacc
     1741 gcaatctcgc ttctcacatg ccggagccgc tccagatggc ggcggacttc ttcataccac
     1801 agggccatct tcagaccaaa tgagattggc tccccgtgaa tgccgtgtga ccgtcccacc
     1861 atcacctgat tcttgtagcg gaacgcctgc tgtttcagca cgacgaggag ctcatcaact
     1921 ccccccaaga tcagatcgag cgcctcggtc atttgtatgg cgagtgacgt atcgactatg
     1981 tccgaagatg tgagtcccat atggagaaag cgatgttccg gtcctaccgt gtccatgagc
     2041 gattcgagaa aggcgatcac atcgtgtttc gtaatctttt cgatctcggc aatccgttcg
     2101 acatcgatct tggccttctt cctgattttc gccgccgttc cacgcggcgc ctgcccggcc
     2161 tgctcaaagg ccgcacaggc ctgcagttcc acctccagcc agatctcata tttatgcttc
     2221 agatcccaga tcgctttcat ccggggacgt gtatagcgtt caatcactat ccgctctcct
     2281 ccagatcccc cgatccgggt ccgcccgctt cgcagccaac cgcaactcgg tggccaatgc
     2341 cttatccaga accgcgttca cgaacttcga ggcatcgtta tcgccgaaac tcttcgcgag
     2401 ttcgatggct tcgtccatcg taaccttcgc aggcacttca tccagccaca acaattcata
     2461 c
//
LOCUS       DATMTI010000391         5532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_439138, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000391 DATMTI010000000
VERSION     DATMTI010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439138"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VN039_06430"
     CDS             <1..222
                     /locus_tag="VN039_06430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWV45643.1"
                     /translation="IVLLSGGTEAADPVNRSLRSGLHDAITTDNTLAIGKILFSTYLF
                     PFEVASLVLLVAMIGAIVLAKRDIGESKT"
     gene            295..597
                     /gene="nuoK"
                     /locus_tag="VN039_06435"
     CDS             295..597
                     /gene="nuoK"
                     /locus_tag="VN039_06435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWV45644.1"
                     /translation="MIPISYYLILSAIVFLTGVVGVLIRRNIIAILLSVELMLNATNI
                     NFVAFSDHLQDLGGQVFVFFALTVAAAEVAVGLAIIIALHRSRSTINVEEFNLLKW"
     gene            691..2592
                     /gene="nuoL"
                     /locus_tag="VN039_06440"
     CDS             691..2592
                     /gene="nuoL"
                     /locus_tag="VN039_06440"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWV45645.1"
                     /translation="MLYALIPLLPLAAFLVLGLGGWHIKDRAHLVAVPAVVLSLVLSV
                     AAFLEVAVSGSVISVPLYTWLTSGHLDIHIGLHIDRLAAVMLLLVTGVSSLVHVYTIG
                     YMHGEPGYARFFSYIALFTFSMLMLVLADNLLQLFVFWEAVGLCSYLLIGHWYERASA
                     CAAATKAFVVNRVGDFGFMLGLLLVWYSFGSFNYHEIFPAAHEAGEFTMNLLGPFGGT
                     WEVSVFTVIGLLLFAGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVA
                     RLAPIYNLSPTAMSVVAVTGAATMVLGATIALTQTDIKRVVAYSTVSQLGYMIMACGL
                     GAYASGMYHLLTHGAFKALLFLGCGSVIIALHHEQDMRHMGGLKNKLPITYWTFVIGS
                     LALAGFPLTAGFFSKDDLLVSAWSSGDLGQILTLFGLLTALLTAFYSFRLVFVIFWGT
                     SHIDPHHAEHLHEPPQTITTPLIILACFSILTGYLGIPSFLEPVFSTGSEAAAHHGSA
                     GLAIMITATVMGLIGIAAAYYVYVLNPDLPERLARQLGSLYRGSLNKWYVDEAYDRLF
                     VRPTFAAASELWKRVDVNLIDGAVNGIARGVAWGGWLLRLVQSGQTQHYALAMAAGIV
                     VIVTIFLIS"
     gene            2754..4328
                     /locus_tag="VN039_06445"
     CDS             2754..4328
                     /locus_tag="VN039_06445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWV45646.1"
                     /translation="MTHSFPWLTVLILLPLIGAAAVFFVKDPSARLVALAVAVADLLI
                     SLPLWWLFDASSGNMQFMESALWIASPPIHYRLGLDGISLPLVLMTTVLMPLCILISW
                     HSIESKVQSFMAMLLIMEGAMIGVFSALDFVLFYVFWEAMLIPMYLLIGVWGGPNRLY
                     AAIKFFLYTLTGSVLLLVAILVLYFQGGHTFDILQLSQGTYSKSLQFWLFLAFFAAFA
                     VKVPMFPFHTWLPDAHVEAPTAGSVILASILLKMGTYGFVRFSLPMLPDASQAFTPLM
                     VVLSIIAIIYGAYMALAQPDLKKLIAYSSVSHMGFVTLGLFMFNIQGIEGAVMQMVNH
                     GITTGGLFLCVGIIYERTHSRQIADNIGLTKPMPRYATFLVIFALSSLGLPGTNSFVG
                     EFMVLIGTFLWSKIATAFASLGIILAAAYFLWMVQRVAFGVPAPHMLPKLRDVDLREM
                     VTLVPLVVLIFAIGLFPNPILTRMHPSVEKVIARVFPPVVEQISTVSQGFHNDAGSPH
                     GEAKGFTSTEAEGRQP"
     gene            4325..>5532
                     /locus_tag="VN039_06450"
     CDS             4325..>5532
                     /locus_tag="VN039_06450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWV45647.1"
                     /translation="MTFSVGDLFLILPELLVITAACIVFVLDPVLRPSDKDGLVWLSL
                     GTLAICMGLTASQMRAPVEAFSGLVVIDTYGAFWKLLLYFVTGLTILLSHSYLKEERL
                     YFGEYYGFVLLALSGMMVMVSAADLLTIYLGTELMSLSLYVMAGLKRSEPRSLEASAK
                     YFVLGAFSSGILLYGISLLYGATGSTRLPAIAAAIAGQSLDDPLLMFATILLAVGFGF
                     KLAVVPFHMWTPDVYQGAPTSVTAFMAVASKAASFGAFLRVFIEGLGGLKANWSAIFL
                     LLCLATLVLGNVVALVQTNIKRMLAYSSIAHAGYALIGVVAAGRLANSSGISSVLLYL
                     ALYTFMTFGAFAIVAMLRKDGIEGNEIEDFTGLSKRHPVAALLMLIFMVSLAGIPPTA
                     GFIGKFYVFMS"
BASE COUNT          985 a         1640 c         1485 g         1422 t
ORIGIN      
        1 atcgtgctgc tctccggcgg aaccgaagcg gccgatccag tcaatcgatc gctccggtcc
       61 gggctgcatg atgccatcac caccgacaat acgttggcca tcggcaagat acttttttca
      121 acctacttat tccctttcga agtggcctcc ttggttctct tggtggccat gatcggcgcc
      181 atcgtcctcg cgaaacgcga catcggagag agcaaaacgt gaagagtcaa tggttcatga
      241 tgaaacccat gccatctttt tcccatcaac cattcaccgt gaaccattga acccgtgatt
      301 cccatttctt actacctcat cttgagcgcc atcgtcttcc tgacaggcgt tgtgggcgtc
      361 ctcatccggc gcaatatcat cgcgattctg ctgtcggtgg agctgatgct gaacgcaacc
      421 aacattaact tcgtcgcatt ctccgaccac cttcaggatc tcggcggtca agtattcgtc
      481 ttttttgctc tcactgtggc cgctgcggag gttgccgtcg gacttgcaat tatcatcgcc
      541 ttgcaccgat ccagatccac aatcaacgtg gaggagttca acctgttgaa atggtagcta
      601 attcgcagat ggcttatagc gtatggcgaa tggcccgaac gagagtccga ggttcgtgcc
      661 atcagctatc agccatcagc tcctgatcct atgctgtacg ccctcatccc actccttcca
      721 ttggccgcct ttctcgtcct aggattaggc ggttggcaca tcaaagaccg cgcccacctt
      781 gtcgcagttc cggcggtcgt gttgtcgctc gtcttgtcgg tggcggcatt tttagaagtg
      841 gcggtgtccg gctccgtcat ttcggttccg ctgtacacct ggctgacatc cgggcatctc
      901 gacattcaca tcggtcttca tatcgaccgg ctggccgccg tcatgctcct gctggtcacc
      961 ggtgtcagct cgctcgtaca cgtctatacg atcggctaca tgcacggcga gccgggctat
     1021 gcccgtttct tcagctacat cgccctgttc acgttttcca tgttgatgtt ggtattggcc
     1081 gacaacttat tacagctctt cgtgttctgg gaagccgtgg gactgtgctc ctatctgctg
     1141 atcggccatt ggtacgaacg tgcttccgcc tgtgcagcgg cgaccaaagc ctttgtcgtg
     1201 aatcgcgtgg gcgatttcgg attcatgctg ggccttctgc tcgtctggta cagctttggg
     1261 tcgttcaact atcacgagat cttccccgcc gcacatgagg cgggagagtt cacgatgaac
     1321 ctccttggcc ctttcggggg aacatgggaa gtttccgtgt tcacggtcat cggactcttg
     1381 ctgttcgccg gcgcggtggg taaatcggca caagttccgc ttcacgtctg gctgcctgac
     1441 gcaatggaag gacctacgcc gatttcggcc ctcattcacg ccgccacgat ggtcacagcc
     1501 ggcgtcttca tggtggcgcg tctcgccccg atctataacc tttctccgac cgcgatgagc
     1561 gtggtcgctg ttacaggcgc ggcaacgatg gtcctcggcg ccacgattgc gttgactcaa
     1621 accgacatca aacgggtcgt cgcgtattcg acggtcagcc agctcggtta tatgatcatg
     1681 gcttgcgggc tcggagccta cgcctccggc atgtaccatc tactcaccca tggcgcattc
     1741 aaagccctgc tgttccttgg ctgcggctcg gtcattatcg cgctgcatca tgaacaggac
     1801 atgagacaca tgggcggcct caaaaataag ttgccgatta cctactggac gtttgtgatc
     1861 gggtccctgg cgctcgccgg cttccctctg acggcaggat tcttcagcaa ggatgacctt
     1921 ctcgtgtccg cctggtcgtc gggagacctt ggtcagatac tgacgctctt cggtctgctg
     1981 acggctctcc tgaccgcctt ctacagcttc aggctcgtgt tcgtaatctt ctgggggacc
     2041 tcccatattg atccgcacca cgcggaacat ctccacgaac ccccgcaaac gatcaccaca
     2101 ccactcatca ttctggcatg cttcagtatt ttgaccggtt atctcggtat cccgtcattt
     2161 ctggagccgg tcttttcaac cgggagtgaa gctgctgccc accatggttc agctggtctt
     2221 gccattatga ttacggcgac tgtgatgggt ctgatcggca tcgccgcggc ctactatgtc
     2281 tatgtactca acccggatct ccctgagcgc ttggcccgac agttaggcag cctgtatcgc
     2341 ggttcgttga ataaatggta tgtcgacgaa gcctatgacc gcctgttcgt tagaccgaca
     2401 ttcgcagccg cgtccgagct ctggaagcga gtcgacgtca atctcatcga cggagcggtc
     2461 aacggcatcg cgcgcggtgt tgcgtggggc ggatggttgt tgaggctggt ccagagcggg
     2521 cagactcaac attacgcatt ggcgatggcg gccgggattg tcgtcatcgt gacgatcttt
     2581 ctgatttcgt aggagaaagt caccggtcac cgatcattag tcgggtgtca tgggagagcg
     2641 cgatggcgct gctctttcgt tccatcgtcg atcgactagt gaccggtaac cattaaagat
     2701 ggaccattga ctaacgaccc gcccgtggcg aggtctcaga cggctattga ccgatgaccc
     2761 attcctttcc ttggcttacg gtactgattc ttctcccgct gatcggagca gctgcggtct
     2821 ttttcgtgaa ggacccctcg gctcggcttg tcgccctcgc cgtcgccgtc gctgacttac
     2881 tgatctcact cccgctctgg tggctgttcg atgcctcctc cggcaacatg cagttcatgg
     2941 aatctgccct gtggattgcg tcgccgccga ttcactatcg gctgggtctc gacggcatca
     3001 gtctgccgct ggtcctcatg acgacggtcc taatgccgct ctgcatcctg atttcttggc
     3061 actccatcga atcgaaggtc caaagcttca tggccatgct gctgatcatg gaaggcgcca
     3121 tgatcggcgt attctcagcc ttggattttg tgctgttcta tgtgttttgg gaagcgatgc
     3181 tgatcccgat gtatctcttg atcggagtct ggggcggacc gaatcgtctc tatgccgcga
     3241 ttaaattctt tctctatacc ctgaccggca gcgtcctgct gctggtcgcg attttggtgc
     3301 tgtatttcca gggtggccat acgttcgaca tccttcaatt gagccaaggc acctattcca
     3361 agtctctgca attctggctg ttcctggcgt tcttcgccgc attcgccgtc aaggtcccca
     3421 tgttcccctt ccatacctgg ttgccggacg cgcacgtgga agctccgacg gcaggcagcg
     3481 tgattctcgc cagcatcctg ctcaagatgg gcacctatgg attcgtgcgc ttcagtttgc
     3541 ccatgttgcc ggacgcctct caagcgttta cgcccttgat ggtggtcctc tcgattatcg
     3601 ccatcatcta tggcgcatat atggcgttgg cgcagcccga cctgaaaaag ctcatcgcct
     3661 attcgagcgt gagccacatg gggttcgtca cgctcggcct attcatgttt aacattcaag
     3721 gaatcgaagg cgccgtgatg cagatggtca atcacggcat caccacgggg ggactctttc
     3781 tctgcgtggg gataatttac gaacgcaccc acagccgaca gatcgcggac aatatcgggc
     3841 tgaccaaacc gatgccacgg tatgcgacgt tcctggtcat ttttgcattg tcttcgttgg
     3901 gattgcccgg cacgaacagt ttcgtcgggg agtttatggt tctgataggc accttcctct
     3961 ggagcaagat cgccacagcc ttcgcctcat tgggaatcat cctcgccgcc gcctacttcc
     4021 tctggatggt tcaacgtgtc gcattcggcg tgccggcccc gcacatgctt ccgaaactac
     4081 gtgatgtcga tctgcgtgaa atggtgaccc tggtgccgct cgtcgtattg atctttgcga
     4141 tcgggttgtt cccgaacccc atcctgaccc gcatgcaccc aagcgtggag aaagtcatcg
     4201 cgcgcgtctt cccgccggtc gttgagcaga tttcaaccgt aagccaaggg tttcacaacg
     4261 atgcgggctc gcctcacggc gaagccaagg gcttcacgag caccgaggcg gagggaagac
     4321 agccatgacc ttctcggtcg gagatctatt cctcattctg ccggagctgt tggtcattac
     4381 cgccgcctgc atcgtgtttg tgctcgatcc ggtactgcgc ccatccgaca aagatgggct
     4441 ggtgtggctg agtttgggaa ccctcgccat ttgcatgggc ttgacggcct cgcagatgag
     4501 agctccggtc gaggcgttca gcgggctcgt ggtcatcgac acctatggag ccttttggaa
     4561 actgctgctc tactttgtca ccggtctcac gattctcctt tcgcattcgt acctcaagga
     4621 agagcggctg tacttcggcg aatactatgg ctttgtcctg ctcgccctct cggggatgat
     4681 ggtcatggta tcggcagccg acttgctgac gatttatctc ggtactgagc tcatgtccct
     4741 ttcgctctac gtgatggcgg ggctgaagcg atctgaacct cgctcgctcg aagcgtcggc
     4801 aaaatacttc gtactgggcg cattttcatc cggtattctc ttgtacggca tctcactcct
     4861 ctatggcgcg accggaagca ccaggcttcc ggccattgcc gcagcgatcg cgggacagag
     4921 cctggacgat cccttgctaa tgttcgcgac aatcctattg gcagtcggat tcgggttcaa
     4981 actcgccgtc gtaccgtttc atatgtggac accggacgtc tatcaaggcg ccccaacttc
     5041 agtcaccgcc ttcatggccg tggcatccaa agccgccagc ttcggcgcgt tcctccgggt
     5101 atttatcgag ggactcggcg gcttgaaggc caactggtcc gccatcttcc tcctgctctg
     5161 cctcgccacc ctggtgttgg ggaatgtcgt ggcattggtg cagacgaata tcaagcggat
     5221 gctcgcctat tcgagtattg cccatgccgg ctacgccttg atcggcgtgg tggcggccgg
     5281 acggttggca aactcttcgg gcatctcaag cgtattgctt tatctcgcgc tctacacatt
     5341 catgaccttc ggcgcattcg ccatcgtcgc catgcttcgc aaagatggaa tcgaaggaaa
     5401 tgagatcgag gacttcacgg gtctgtccaa acgccatcca gtggcggcgc tgctcatgtt
     5461 gattttcatg gtctccttgg cgggtattcc tcccactgcg gggtttatcg gaaaattcta
     5521 cgtgtttatg tc
//
LOCUS       DATMTI010000392         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_736338, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000392 DATMTI010000000
VERSION     DATMTI010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736338"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..853)
                     /locus_tag="VN039_06455"
     CDS             complement(188..853)
                     /locus_tag="VN039_06455"
                     /inference="COORDINATES: protein motif:HMM:NF018691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWV45648.1"
                     /translation="MAVVSACSTKGGVGKTTTVICLADTFARGGGSVAILDADPNGHV
                     RTWRERAPADCKVDVVADLTENNLLDRIAEAAETYQLVLVDLEGAASQSVTYAIAQSD
                     LVLIPTKVSGMDLQEVFRTYEVVQRAEKMLRRPIPARAIFTQMSPLMSRVALHARNEI
                     TTAGVPVITTEIIHRAAYQAIHFSGETPASPNGDPKAAREMNSAMVEILEILASPHIA
                     AAE"
BASE COUNT          514 a          421 c          568 g          313 t
ORIGIN      
        1 cgtcttatcg ggggcggacg ccccctcccc taccgggctg gtcgaggcgc gcttgaagcc
       61 gaggtcggca tttgcgtcga cgagcgcctt tgcagtctcg gcgttgagga ggcctgaagc
      121 gaggccgcgg gggcggggcg caggcataag gtttggattg ctggtcatga ttacggccct
      181 cctttgtcta ttccgcagcc gcgatgtgcg gggacgctaa aatctcaagt atctcgacca
      241 tcgcggaatt catttcacgg gcggccttgg gatctccatt aggcgaagcg ggcgtttctc
      301 ctgaaaaatg aatggcttga taggcggcgc gatgaatgat ttcagtcgtt atgaccggaa
      361 cgcccgccgt cgtgatctcg ttgcgggcat gcagcgccac acgcgacatc agaggcgaca
      421 tctgggtgaa gatcgcgcgg gcgggtatcg gacgacgcag cattttctcg gcgcgttgaa
      481 caacctcgta ggtacggaag acctcctgaa ggtccatgcc ggagaccttt gtcgggataa
      541 ggaccagatc gctttgagca atggcgtagg tcacggactg ggaggccgcg ccttccaggt
      601 caacaaggac gagctgatag gtttccgccg cctcggcaat gcgatcgaga agattgtttt
      661 cagtcaggtc ggcgaccaca tcgactttac agtcggccgg cgcgcgttcg cgccaggtcc
      721 tcacgtggcc attgggatcg gcatccagaa tcgcgacgga gccgccgccg cgtgcaaagg
      781 tgtcggcaag acagatgacc gtggtcgtct tgccgacgcc gcctttggtt gaacatgcgc
      841 ttacaacagc cataagaaat atctcccttt gggcacatgc ctgttctgac tgaagcggca
      901 gaaaggcaaa atggaggaac tgcttaatgc cgcttagggt gatttggtaa aaagcgcatt
      961 aaatatattc gccaaaatgc ctgaaacgac aaaaatataa gtgggaaaag gggaaaaggg
     1021 gaaaaagagg aaagcaggaa aacggaagag tgtataaaag ggcaaaaagg aaaacgtgca
     1081 aaggtgaaaa caggcaaacg ctcatgatgg gtggtcggga gaaagaatgc taaaaattca
     1141 gacgataacc agtcagtcga gggtttcgac aggcgcttaa gtcggaaagg tgtcagaggg
     1201 caatgtggca aaagggcaaa ccggaaaaac gtaatcacgc aataacggcg tttaggtggt
     1261 agcgcacaaa cggcgaaaag gctaaaaggc aaacggagaa caaggcaata aaggaaagag
     1321 gcaagtcggt gaatagccat gatgtgcaaa aaagcaaaag ggcaaacagg caaaccgaca
     1381 ttattggtat gaggtgaaat ctggcatcaa ggcattgggg agacgtggcc aatgtacaag
     1441 aggagaaaca ggacagggga gggactgctg ggccggacaa accggcaaga cggaatcgtg
     1501 ccttaaaggc agggcgatga cggcatcctg caagcaatag cggcgctgca atgggccgaa
     1561 aaccactggc tggggggcac atgaccgaag acggaccgac cgcaccaggc ctcgggctat
     1621 ccaaggtcga ggagaagacc tgggcaaact ggaaaaaggg caaataggca tcgtggcaaa
     1681 acggcatact tccaatgcta cgaataccca aatgtccaaa tgccgcaaaa ggcattacgg
     1741 caaatgtgaa aaagggcaaa cagccaacac tgacaggtca ccaaagggta atgtgggaga
     1801 agatcatgca atacct
//
LOCUS       DATMTI010000393         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_736347, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000393 DATMTI010000000
VERSION     DATMTI010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736347"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2088)
                     /locus_tag="VN039_06460"
     CDS             complement(<1..>2088)
                     /locus_tag="VN039_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45649.1"
                     /translation="WPRLLEILARHAQSGMGAALCRSLSLSSDLAEARLRQQETTEMV
                     SLLEGSDPMPTLAFPDIREQLTRSRKGGALEAGELRDCAVVLALMAEVERYAESHTVE
                     SQALARVLAPLHEAKSMRGILKAIEEAIQSDGFMKDTASPELRRLTHQAQGLKQEMRQ
                     HLKQLLHSQRYEDVLQESYFAQREGRYVVPVKADMRGRIPGIVHDVSASGATVFLEPR
                     ELVELNNSIKVTDLEIEREVQRILRELTALVASNADTIEQGIKVLAECDVIRARAEMS
                     RRLRCNPVVLNEQGRVVLKQARHPLLLIAKDQVVANDIHMDETVRILVISGPNTGGKT
                     VTLKIVGLFALMVRAGLHLPCAPESEMALFTHLYADIGDAQDLSRDLSSFSAHMMYMI
                     RLLSESISVPRSLIPLTPCALVLLDEPVTSTDPQEGAALAEALLCRLAELNMKVVATT
                     HYGALKELAQTTPGFVNASVEFDVERLAPTYRLFMGIPGGSSALEIAGRLGMDHSILN
                     DARKRLHHDNQRLDELMADLQRKQRQLAEDNEKAQSARQEAEQAAREAQMLRAQLQEA
                     EQEARRGLKKKLGEQFQRARAEVQATVDSLKREQKLIKAKETKQRLHELETKVRQDLV
                     PAGKTISLEQLGIGDTVEIVGLGMTGSLLETTQGKKRVRVKVGDGEILATVSNLVGLA
                     HEASTEIAAPTSAS"
BASE COUNT          431 a          642 c          528 g          487 t
ORIGIN      
        1 gacgctgaag tcggtgctgc tatttctgta ctggcttcat gcgcgagacc gacgaggttc
       61 gaaactgtag ccaaaatttc gccatctccc accttgaccc gcacgcgttt ctttccctgt
      121 gtcgtttcca acaaactccc cgtcatgccc aacccgacga tttccaccgt atcgccgata
      181 ccaagttgct caagtgagat cgtcttgccg gccggtacaa gatcctgccg gactttcgtc
      241 tccaactcat gcaatcgctg ttttgtctcc ttggccttga tcagcttctg ttcgcgctta
      301 agggagtcga cggtggcctg gacctcggcc cgagcccgtt gaaattgttc gccgagtttt
      361 ttcttgagcc cacgccgggc ctcctgctct gcttcttgca gttgcgctcg aagcatttgt
      421 gcttcccgag ccgcttgttc ggcttcttgt ctagcgctct gagccttctc attatcttca
      481 gccaattggc gttgtttccg ctgtaaatcg gccattaact catcgagtcg ttgattgtcg
      541 tgatgcaacc gctttcgcgc atcgttcaaa atgctgtgat ccatacccag acgaccggcg
      601 atctccaagg cggatgaacc gccgggaata cccatgaaca gtcggtaggt cggcgccaac
      661 cgctccacat cgaactccac gctggcattc acaaaaccag gtgtcgtttg cgccagttcc
      721 ttgagtgcgc cgtaatgcgt ggtcgccacc actttcatgt tcagttcggc tagacggcag
      781 agaagtgcct ctgccagtgc cgccccttct tgcggatcgg tcgaggtcac aggctcatca
      841 aggagcacga gtgcgcacgg tgttaagggt atgagtgatc tagggacgga gatgctctcg
      901 gaaaggagtc ggatcatata catcatgtga gcggaaaagc tcgacaaatc acggctcaaa
      961 tcctgagcat ccccgatgtc tgcatagaga tgggtaaaaa gcgccatctc tgactccgga
     1021 gcacaaggga gatgcagtcc ggcccgcacc atgagcgcga acaacccgac gattttgagc
     1081 gtgacggtct tcccgcccgt attcggcccg gagatgacaa ggatacggac cgtttcatcc
     1141 atgtggatgt cgttcgcgac aacctgatct ttcgcgatga gcaacagcgg atgccgcgcc
     1201 tgcttaagga ccacacgacc ttgctcattc aacacgacag ggttgcacct gagccgacga
     1261 ctcatctcgg cccttgctct gatcacatca cattcggcca gcaccttgat cccttgctca
     1321 atcgtatccg cattggaggc cactagcgca gtgagttccc gtaagatgcg ctggacctca
     1381 cgctcaatct ccagatccgt caccttgatg gaattattca gttcgaccaa ctctcgcggc
     1441 tccagaaaga cggtcgcccc actggccgac acatcgtgga cgatcccggg gattctcccc
     1501 cgcatgtccg ccttcaccgg cacgacatag cgtccttctc gctgggcaaa atacgactct
     1561 tgaagtacat cctcatagcg ctgagaatga agaagctgtt tcagatgctg ccgcatctcc
     1621 tgtttcagtc cctgagcctg gtgagtaagg cgtctcagct ccggcgaagc cgtgtctttc
     1681 atgaaaccgt cagattggat cgcttcttcg atagccttca ggatcccgcg catacttttt
     1741 gcctcatgaa gtggagccaa gactcgagcc agggcctgac tctcgactgt atgagattca
     1801 gcatatcgct ctacttctgc catgagggcc aacacaaccg cacagtctcg taattcaccg
     1861 gcctcaagcg ctcctccctt cctagatcga gtcagctgtt cgcgaatatc gggaaaggcg
     1921 agcgtcggca tcggatcgct tccctccagc aaactcacca tctcagtcgt ctcttgttga
     1981 cgcaggcggg cctccgcgag gtcgctggac aaggaaagcg accgacatag agcggctccc
     2041 atccccgatt gcgcatggcg cgcgagaatt tccaataacc ggggccac
//
LOCUS       DATMTI010000394         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_524538, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000394 DATMTI010000000
VERSION     DATMTI010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524538"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VN039_06465"
     CDS             <1..564
                     /locus_tag="VN039_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWV45650.1"
                     /translation="LFLAAIACSGCQTELPPLPYQVGPYTTVRLSPGDTIKVAFAENS
                     DLDQTEKIRRDGKITLPMIGEVQAAGRTVMQLQNILISRYSDSLDNSEVLVTLENGNA
                     TAIVAGFANNPGKVEFDRPTTVYQAVMQAGGVSDYGSTSNIHLTRIINGQQLSQTVNL
                     RPTIHGEPTRPTYVQDGDVIYVGRSWF"
     gene            909..>1742
                     /locus_tag="VN039_06470"
     CDS             909..>1742
                     /locus_tag="VN039_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45651.1"
                     /translation="MEQTDIRAIGVAHFSQFLDVGTDDKAGNLTLAMTHNFSQRLTVS
                     DDLFASYQTEPDFAADVGPENVRSPHYFITNVAAITYHWLPRLAFVTSYNFQRVKYVE
                     NSAQTDFQNRFDNLLSEEAVFNLTRRTILLGEYRFEDTEYDTAPLNSTTHYAIAGVDH
                     HLTEHFDARLLGGATFRVFENGGERTDPYAEADFLYQGGNHTLGWRTSYRVEQPSVQP
                     AVARQTIRTGLTLTYNLTSRLSSTSAFFFHHDENEGASQNTTTSTQDSLELVLGFRYT
                     IN"
BASE COUNT          409 a          485 c          464 g          384 t
ORIGIN      
        1 ctgtttttgg ccgcaatcgc ctgcagcgga tgccagactg agttgccgcc attgccgtat
       61 caggttgggc cgtacacgac tgtgagactt tccccaggag acacgataaa ggtcgccttt
      121 gccgagaaca gcgatctcga tcagacggag aagattcgcc gagacggcaa gatcacgctg
      181 ccgatgattg gagaggtgca ggctgccggc aggacggtga tgcagcttca aaacatcctg
      241 atcagccgct acagcgattc tctcgacaat tcggaagtgt tagtcacact ggagaacggc
      301 aatgctacag cgatcgtggc cggctttgcg aacaatccag gcaaagtgga gttcgaccga
      361 cccacaacgg tgtatcaggc ggtgatgcag gccggggggg taagcgatta tggaagtacc
      421 agcaacattc atctaactcg gatcatcaat gggcaacagc tctcccagac cgtgaacctg
      481 cggccgacca tccatggtga accgaccaga ccaacttatg tccaagacgg agacgtgatt
      541 tacgtggggc gcagctggtt ttgaactgca ctgccggtcc gcgcgattta ccggccatga
      601 gtctgccatg tcgaactcgc caatttcgaa accgtatccg ctctctctgc gtcgttttgc
      661 tgtgtctcct ttggttggga gctgctcttg atcgagcatt agcggccggt caatacagct
      721 cgggtgcgca accgggcgcc acgccgcaat cggccacgac agcgaaagcg caacgtattc
      781 cgcttgaaat cacagccggc gtagacctcg gttacgatga caacgtcatc ggaaccaatt
      841 ccgttaactc gaacggtgga ggttcgtttt ttacccggga aagtttagtc ctctcgtacg
      901 atcgaccgat ggagcagacc gatattcgtg caattggggt cgctcatttc agccagtttt
      961 tggatgtggg cacggatgat aaggccggaa acctgacgct ggcgatgacg cataactttt
     1021 cgcagcgcct tacggtgagc gatgacttat tcgccagcta tcagaccgag ccggacttcg
     1081 ccgccgatgt tggtccggag aacgttcgtt cgcctcacta cttcatcact aacgtcgccg
     1141 cgatcactta tcattggctg ccccggctcg cgttcgtaac cagctacaac ttccaaagag
     1201 tgaagtacgt cgagaactcg gcccagaccg atttccaaaa ccgcttcgat aacctgcttt
     1261 ccgaggaagc tgttttcaat ctcacccgca gaacgatact acttggagag tatcggttcg
     1321 aagacacgga gtatgacact gcgcctctga attcgacaac acattatgcc atagccggtg
     1381 tcgatcatca tttaaccgag cacttcgatg ctcgcctgct tgggggagca acatttcgtg
     1441 tttttgaaaa cggtggggag aggaccgacc cgtacgccga agcggacttt ctataccagg
     1501 gcggcaacca tacgttgggt tggagaacga gctatcgagt tgaacagccc agtgtgcaac
     1561 cggcagtcgc gcgacagaca attcgcaccg gtttaacgct cacgtacaat ctaacttccc
     1621 gtctcagctc aacgagcgcg ttctttttcc accatgacga aaacgaagga gcttcccaga
     1681 acacgacgac cagtactcaa gactctttgg agcttgttct cggtttcaga tacaccatca
     1741 ac
//
LOCUS       DATMTI010000395         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_228604, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000395 DATMTI010000000
VERSION     DATMTI010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228604"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1337
                     /locus_tag="VN039_06475"
     CDS             321..1337
                     /locus_tag="VN039_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HWV45652.1"
                     /translation="MPHMTAVQLRSPGAAFEVIKREIPIPGANQVRIKVQACGICHSD
                     LFVKEGYWPGLLYPRVTGHEVAGLVDEVGTGVTTWTRGQRVGVGWHGGHCGQCDPCRR
                     GDFMGCRNFRVTGFHDDGGYAQYMIARSEVLAAIPDSISPAEAAPLLCAGVTTFNSLR
                     HSGAVAGDLVAVQGLGGLGHLGIQFASKMGFRTVAIGRGKDKESLALKLGAVRYLDTD
                     AVDVTKELASLGGASVILATAPDSKAMSALIDGLGGGGKLLVVGASTDPLSVTPVQLI
                     MSRRSITGWPSGTARDSEDTLNFCALTGIRPMVETLPLEQAATGYERMLSGKMRFRMV
                     LTMN"
     gene            complement(1415..1660)
                     /locus_tag="VN039_06480"
     CDS             complement(1415..1660)
                     /locus_tag="VN039_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45653.1"
                     /translation="MDFVYTHGMSDRSDSFVFFSTESRRIFYEDHRSLALFMILVVFA
                     APFAGLYVAGLLGVVVGVLVSAAAYVLTPCLWKWLGG"
     gene            complement(1715..>2337)
                     /locus_tag="VN039_06485"
     CDS             complement(1715..>2337)
                     /locus_tag="VN039_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HWV45654.1"
                     /translation="ALIQPSEWMPLIFNDQDAGYETQDEAERVLQAMMALYNDCIRER
                     TVGGVSLPPGCEVRPWAMDNLDVEAPLSQWAQGFGIGYDYLVEVWNEYTPDELDEELG
                     AVLMTLTFFTSPHLAQAYHQETKGKGSLDQLSQSVMEIFPEAMREYAHLGRSIYQARL
                     EVGNLNLEHSAHIKIGRNDPCPCGSGKKYKKCCGLTKGTSSGQVFH"
BASE COUNT          527 a          663 c          652 g          495 t
ORIGIN      
        1 agtggaagtc ccagttctat tccgttcaca ccgcagccta acgtcactct gctaacgaca
       61 attccactca tcactaattt cgcgaccgcg ccgacccgag cgttgtcggt caagacagca
      121 atagatattc accaattcag cttcgttatg ccgactacga agcgatgcct cgcgagaaat
      181 tggcaacgga atcgaaccgg tccttttcaa caccgaggtt tggaccgaga tttggcgcgt
      241 atgccaatgg tctggtacag ttcacaacgg ttgacaagcc ggtggacgat acctaattac
      301 aagcctggaa ggaggccgac atgccacaca tgaccgcggt tcaacttcgc tctccgggcg
      361 cagcattcga agtcatcaag cgagagatcc cgataccagg agcgaaccaa gtccgcatta
      421 aagtacaggc ttgtgggatt tgtcatagtg acctgtttgt gaaggaaggc tactggcccg
      481 gcctgctcta cccacgggtc acaggccacg aggtagccgg gcttgtcgac gaggttggaa
      541 ccggcgtgac gacatggaca agagggcagc gagtgggtgt gggctggcat ggtggccatt
      601 gtggtcaatg tgatccctgt cggcgtggcg acttcatggg atgccggaat ttccgtgtca
      661 caggattcca tgatgacgga ggatatgctc agtatatgat cgctcggagt gaagttctgg
      721 ccgccattcc ggattcgatc tctcctgccg aggcagcgcc gctcctgtgt gcgggcgtga
      781 cgacgttcaa tagcttgcga catagtgggg cggtcgcagg agatctcgtg gccgtgcaag
      841 gcttgggcgg acttggccac ctggggattc agtttgcgag caagatggga tttcgtactg
      901 tcgcaatagg tcgaggcaaa gacaaggaat cccttgcact gaagctcgga gccgtccgat
      961 atctggacac agatgcggtc gacgtcacaa aagaactcgc aagcctcggt ggcgcttctg
     1021 tcattcttgc gacggctccg gacagcaagg ccatgtcagc gcttattgat gggcttggcg
     1081 gtggagggaa actgctcgtg gtcggggcct cgaccgatcc cctcagcgta acgcccgttc
     1141 agcttattat gagccgccgg tccattaccg ggtggccatc agggaccgct cgagactctg
     1201 aagacacgct gaacttttgc gctctcacgg ggattcggcc tatggtcgaa acgctcccgc
     1261 tcgaacaagc cgctaccggc tatgagcgta tgttgagcgg aaaaatgcga ttccgcatgg
     1321 tgctcacaat gaactagtca atgaggcctt cagggggtcg cgacttcacc ggctgcgagc
     1381 cgatggactt cgacatgtcg gtgtcggaga ctgatcagcc gccgagccac ttccagagac
     1441 agggcgtgag tacataggcc gcagccgaga ccaacacccc caccaccacg cccaaaagtc
     1501 ctgccacata gagtcctgca aacggtgctg caaacacaac caggatcatg aacagggcca
     1561 gcgaccgatg atcttcgtag aatatgcggc gagactcagt tgaaaagaaa acgaaggagt
     1621 cggatcgatc agacatgccg tgagtataga cgaaatccaa cgccctgtct gtccggaagt
     1681 ccccgaggat gagcaccttc agcagtacag tgtgtcagtg gaagacctgg ccggaactcg
     1741 ttcctttcgt gagtccacag cacttcttgt acttcttccc gctgccgcag gggcagggat
     1801 catttcgtcc gatcttaatg tgagctgagt gctctagatt gaggttgcct acttcaagcc
     1861 gcgcctgata gatcgatcgt cccagatgag catactcgcg catcgcctca ggaaagatct
     1921 ccatcaccga ttgggacaac tggtccagac tccccttccc cttcgtttcc tgatggtatg
     1981 cctgggccag gtgcggcgac gtaaagaacg tgagcgtcat cagcactgct ccaagttctt
     2041 catcgagttc atccggcgta tactcattcc acacctccac aagatagtca tagccgattc
     2101 caaacccttg cgcccactga ctcagcggtg cttcgacatc cagattgtcc atcgcccacg
     2161 gtctcacctc gcaccctggc ggaagagaaa cacctccaac ggttcgttca cgaatgcaat
     2221 cgttgtagag ggccatcatc gcctggagca cccgctccgc ttcatcttgc gtctcatagc
     2281 cggcatcctg atcgttgaag atcagcggca tccattcgga aggttgaatc aacgccg
//
LOCUS       DATMTI010000396         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_99692, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000396 DATMTI010000000
VERSION     DATMTI010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99692"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VN039_06490"
     CDS             complement(<1..192)
                     /locus_tag="VN039_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45655.1"
                     /translation="MDPNKIERVYTSYAGFYDKVFGKVFHEGRESAIRNLNVQPDERI
                     LEVGVGTGLALPMYPRHCRI"
     gene            476..1093
                     /locus_tag="VN039_06495"
     CDS             476..1093
                     /locus_tag="VN039_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45656.1"
                     /translation="MILAGFFAGILFIGLIVGDWLYFIRLTPDAIRYGCRVANNPDQW
                     PGTALTTLGDRFTPEGVLMLPHGVAWYYPQLSQIAIRPQYRLFSRRFRTAWPVKGLIH
                     LSPHDQSLNTLCVKRMPWSSALMTLLWFALVLIGSLTFMVQYVMDGGFASLGGTLTGV
                     AVIALAALVLAFGLVTVALAYRLEDSRLMKVYGELRDAVALTTSA"
     gene            complement(1090..1458)
                     /locus_tag="VN039_06500"
     CDS             complement(1090..1458)
                     /locus_tag="VN039_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45657.1"
                     /translation="MSEDSLTTPDPLQTLKAEFRHHLETFYAGLQLAPPYESVEKAIR
                     TLTTMIHGLPIQEQTRILADPGLRWQQFRLAFDASGLAKKHRGIIAGLARHRSTLSLP
                     AEYDHFLNLFSDRKEHHGLG"
     gene            1482..2006
                     /locus_tag="VN039_06505"
     CDS             1482..2006
                     /locus_tag="VN039_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45658.1"
                     /translation="MTKPRDNFSMEECMNRLFTGVCALMGILILAGCSSYRHHHGMMS
                     GGKSEAYWQKGQQDMEGLINRTVKEPDRAKQVNVIAGEIIKEIKTSRERERAYHRDLY
                     TLNASYTAMPDEFSKILNEANIQRMQSSAKVLSLRFKMKDLMTADEWKALTDKMLSYS
                     SRYQHGESGAKTGY"
BASE COUNT          478 a          549 c          568 g          482 t
ORIGIN      
        1 aatccggcaa tgtcgcggat acatcggaag ggcgagcccc gttcccaccc ccacctcaag
       61 aattcgttcg tcaggttgca cattcagatt ccggatagcc gattcacgcc cttcatggaa
      121 gactttccca aaaaccttat catagaaccc cgcgtaagag gtgtacacac gctcaatctt
      181 gttgggatcc atgctctcct ccaaacctca cacgatccgg cccctgcctt cctcaccctc
      241 tcagaggcag cagaccgcta tggcaacgcg gggaaaaatt gaggcgggaa attcatgtag
      301 tgcaaggccc gcccggaacg tagtccggcg tactctagcc aatctcatct tcatgagtca
      361 acagattctt ggagggttcg ggacggaaat accgcctaaa gccgcacagg cagaacggtg
      421 atttcgtgcc ttgcctcctt gattcatgcc tcattcctat gtgatagcta cgtttatgat
      481 actggccggc ttcttcgcag ggatcttatt tatcggacta atcgtcggtg attggctgta
      541 tttcatacgg ctgaccccgg acgccatcag gtacggttgc cgtgtcgcga acaacccgga
      601 tcagtggccc ggaaccgcgc tcacaaccct aggcgatcgg ttcactccgg agggcgtgct
      661 gatgcttcca catggagtgg cctggtatta tccacaactc tcccagattg cgattcgtcc
      721 acagtatcga ttgttctcca ggcggttccg gaccgcctgg ccggtgaaag ggctgattca
      781 tctttcgccg catgaccaat cgctgaacac gctgtgtgtg aagcgtatgc catggtcatc
      841 ggccctcatg acgctcctgt ggtttgcgct cgtcttgatc gggtcgctga ccttcatggt
      901 gcagtatgtc atggatggag gatttgcttc actaggagga acccttacgg gcgtggcggt
      961 catcgccctc gccgcccttg tgctggcctt cggactcgtc acggtggccc tcgcctatcg
     1021 gctcgaagac agccgcctca tgaaagtcta tggcgagttg cgagatgccg ttgcgctcac
     1081 gacttccgcc taacctaacc cgtggtgttc tttcctatcc gaaaacaagt ttaagaaatg
     1141 atcgtattcc gccggcaagc tcaacgtcga tcggtgacga gctaggccgg cgatgatccc
     1201 acgatgcttt tttgcaagac ctgacgcgtc aaaggccaag cggaactgct gccaccgcaa
     1261 gccgggatcg gccaggattc gggtttgttc ttggatcggg agtccatgta tcatggtcgt
     1321 caatgtgcgg atggccttct ccacgctttc atacggagga gcgagttgaa gtccggcata
     1381 aaacgtttcg aggtgatgac ggaattcggc tttcagcgtc tggagtggat cgggtgttgt
     1441 caaagaatct tcggacataa tattacagac gatgatacgg catgacgaaa ccacgtgaca
     1501 acttttccat ggaggaatgt atgaatcggc tgtttactgg ggtgtgtgcg ctgatgggaa
     1561 tactcatact ggctgggtgc tcctcatatc gtcaccatca cggcatgatg agtggtggca
     1621 agagcgaggc ttattggcag aagggccagc aagacatgga agggctgatc aatcgaaccg
     1681 tcaaagaacc cgatagggcg aagcaggtca atgtgatcgc cggcgagatc atcaaggaga
     1741 tcaagacaag ccgtgaacgg gaacgggcct atcaccggga cctttacacc ctgaacgcca
     1801 gctatactgc gatgcccgat gaattttcaa aaattctcaa cgaggcgaac attcagcgaa
     1861 tgcagagttc ggccaaggta ctgagcctcc ggttcaaaat gaaggatctg atgaccgccg
     1921 atgagtggaa ggccctcacc gataaaatgc tgtcctacag cagtcggtat cagcatggcg
     1981 agagtggagc aaaaacaggt tactaaacaa ggttgaggct gagattaagg ttgagttcga
     2041 gggaaattgt agtttttttg ctcagcttaa ccttgac
//
LOCUS       DATMTI010000397         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_651762, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000397 DATMTI010000000
VERSION     DATMTI010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651762"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VN039_06510"
     CDS             complement(<1..1064)
                     /locus_tag="VN039_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWV45659.1"
                     /translation="MIAVVVLLVAPLLAGGLSLVVHRSALLHGINLASMGALVTAEIV
                     ITRTILRDGPFTALDQLVYLDALSTFILFIIGAVGLACSFYMRSYMDDQVAQGVIVAS
                     RLNLFFFLFHMFLLAMVIATIANSVGVQWVAIEATTLATTFLIAFWRRRESLEAGWKY
                     LILCSVGISLALFGVVLLYYSSLHVLSDVSQGLNVTQLLPVADRLNPHVLKLAFIFIL
                     VGYGTKVGLVPMHTWLPDAYMEAPAPVVAMLAGVLEVVAAYAILRVRMIVDHAVPFAF
                     AGGLLALLGFASFVTAAFFLLIQHNYKRLFAYSSIEHMGIAMIGFGVGGPLGTFGGLF
                     HLLNHAFAKSMAFFAAGNIHR"
     gene            complement(1079..1744)
                     /locus_tag="VN039_06515"
     CDS             complement(1079..1744)
                     /locus_tag="VN039_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HWV45660.1"
                     /translation="MQALPDMGSQLVDLCSALLLLTCFAIVAQRRLSACVDLFALQSA
                     FLVVTAALVAYLTGNQHIYIAAALTGVIKVIIIPRVLKKVIDRLNVKRELVMYINVPT
                     GLLICGGLVMLAFFIAQPIIPLGSLLTRDALAIALAIVLIGLFTMIARQKAVTQLVGF
                     LVLENGLFLGATAATYGMPLIVELGVFFDVLIATLIAGIYTNRLQDAFDSVDTDRLTV
                     LKE"
     gene            complement(1749..2699)
                     /locus_tag="VN039_06520"
     CDS             complement(1749..2699)
                     /locus_tag="VN039_06520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HWV45661.1"
                     /translation="MLHALILIVAQTTVLLAISPFLVGVIRKVKARFQLRRGASVLQP
                     YADLAKLFQKQPVISTTTSWIFTATPYIVFASILTAGLLVPTFTSQTPMNFAGNIIAL
                     VYLLALSTFFLILAGLDAGSAFGGMGSSREAIVASLTEPAMILAIFAIALTNGSTNVS
                     TIVHKTALLEDVVTDPSPHLMALAALFIVALAETGRVPVDNPATHLELTMIHEAMVLE
                     YSGRYLALLEWAAGLKLAVFLSLIANVFAPWGIATTITPAAMAFGLLVYLAKTAALAM
                     LIGVIECMFAKLRLFRVTDLLGVAFILALLGLLFFYILRS"
     gene            complement(2703..>2927)
                     /locus_tag="VN039_06525"
     CDS             complement(2703..>2927)
                     /locus_tag="VN039_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45662.1"
                     /translation="VKLETEFLDESRYFARRRHFEFHIEPIFEKYLYDPVVAFFASLA
                     DRLKVIQAGSLHLYLTYIFVALIGLLLLAV"
BASE COUNT          779 a          818 c          808 g          522 t
ORIGIN      
        1 cggtgaatgt tccctgcagc aaaaaacgcc atcgatttcg cgaacgcatg attcaagaga
       61 tgaaacaggc caccgaaggt ccccagcggc cccccgacac cgaatccaat catggcaatc
      121 cccatgtgct cgatgctgga ataggcaaac agccgcttat agttgtgctg gatgaggaga
      181 aagaacgccg ccgtcacgaa tgaggcaaag ccaagcaatg ccaacaatcc accagcgaag
      241 gcaaacggaa cggcgtgatc aacgatcatc ctcaccctca ggatcgcata ggccgcgacc
      301 acttcgagga cacctgcgag catggcgact accggagccg gggcctccat atacgcatcc
      361 ggcaaccagg tgtgcatcgg gacgagcccc accttggtgc cataccccac caggatgaag
      421 atgaaggcca acttgaggac atgcggattc aaccgatcgg ccacgggtag cagctgtgtc
      481 acgttcaatc cttgactgac atcgctcagt acatgcagcg acgagtaata cagcagtacg
      541 acaccgaaca atgcgagcga gatgccgacg gagcacaaaa tgaggtattt ccacccggcc
      601 tccagcgact ctcgtcgtct ccaaaaagcg atcagaaagg tggtagcgag cgtcgttgcc
      661 tcgatggcca cccactgcac cccgacactg ttcgcaatgg tcgcaatcac catggcaagc
      721 aaaaacatat ggaaaagaaa aaagaacagg ttgaggcgtg aggccacaat cacgccttgc
      781 gcgacttggt cgtccatgta cgaacgcata taaaaggagc aggccagtcc gaccgcaccg
      841 ataatgaaca aaatgaaggt cgacaatgca tcgaggtaca ccaactggtc caaagccgtg
      901 aaggggccgt ctctcagtat agtgcgggtg atcacgattt ctgcggtgac gagcgcgccc
      961 atactcgcga ggttgatccc atggagcaat gccgagcggt ggacgaccag gctcagccct
     1021 cccgcgagaa gcggtgcaac caacaagaca acgactgcga tcatcaaggg gccctcgact
     1081 attctttcag cacggtgagt cgatctgtat ccacactgtc gaatgcgtct tgtaaccgat
     1141 tcgtgtagat tcccgcgatc aatgttgcga taaggacgtc gaagaacaca cccaattcga
     1201 cgatcaacgg cattccatat gtggcggcgg tggcacccaa aaacaggccg ttttccagca
     1261 ccagaaagcc caccagttgc gtgacggcct tttgtctcgc gatcatcgta aacagtccga
     1321 tgagcacaat cgcgagggcg atggctaacg catctcgcgt gagcaaagag cccaacggaa
     1381 tgatcggttg ggcgatgaag aaagccagca tgaccaatcc gccgcaaatc aacagtccgg
     1441 tcggcacgtt gatgtacatc accagctctc gcttgacgtt gaggcggtca atgactttct
     1501 ttaggacccg cggaatgatg atcactttaa tgactccggt cagcgctgca gcgatataga
     1561 tgtgctggtt tccggtcaaa taggccacca gggccgcagt cacgaccaga aaggccgact
     1621 gcagcgcgaa caggtcgacg caggccgaga gccgccgttg cgccacgatc gcgaaacagg
     1681 tcaacaagag caaggccgag cacaagtcga cgagttgcga tcccatgtca gggagagcct
     1741 gcattcgttc agctccgaag aatgtaaaag aacagcaacc cgagcagtgc gaggataaac
     1801 gccacgccca gcagatccgt gacccggaac aaccgcaatt ttgcgaacat acattcgatg
     1861 accccgatca acatcgcgag tgcggcggtc ttcgccaagt acaccaggag cccgaacgcc
     1921 atggcggcgg gtgttatcgt cgtcgcgatg ccccatggtg cgaagacgtt ggcaatcaaa
     1981 gagaggaaca ccgctagttt cagaccggcc gcccattcga gtagcgcgag atagcgcccc
     2041 gagtattcaa ggaccatggc ttcatgaatc atcgtgagtt cgagatgtgt cgctggatta
     2101 tcgaccggca cccgccctgt ctctgcgagc gccacgatga agagggctgc caacgccatc
     2161 aggtgcggtg acgggtccgt gacaacgtct tctagcagcg cggtcttatg gacaatggtg
     2221 ctcacattcg ttgatccatt ggtcagggcg attgcaaaga tcgcgagaat catggccggc
     2281 tcggtcagcg atgccacgat cgcttctcgg ctgctgccca ttccgccaaa ggctgatccg
     2341 gcatcgagtc cggcgagaat caagaaaaac gtgctcaacg ccaagagata caccagcgca
     2401 atgatgtttc ccgcaaaatt catgggtgtc tgcgaggtga atgtggggac taggaggccc
     2461 gccgtcaaaa tagaggcaaa tacgatataa ggcgtcgcgg tgaagatcca agaagtggtg
     2521 gtcgaaatca ccggttgctt ttggaagagc ttggccagat cagcataggg ttggaggacg
     2581 ctcgcgcccc gtcgcagttg aaagcgcgcc ttgaccttcc ggatcacgcc caccagaaac
     2641 ggcgagatgg ccagtaagac cgtcgtttgg gccacaatga gaatgagcgc gtggagcatc
     2701 ggttacaccg cgagcaacag caagccaatc agtgcaacga aaatataggt caggtacaga
     2761 tgcagactgc ccgcctggat tactttcaaa cggtcggcca gagacgcaaa gaacgctacc
     2821 acaggatcgt agagatactt ctcaaatatc ggttcaatat gaaattcaaa gtgtcgtcgt
     2881 ctcgcgaagt atcgtgactc atcgagaaac tccgtctcga gtttaac
//
LOCUS       DATMTI010000398         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_651806, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000398 DATMTI010000000
VERSION     DATMTI010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651806"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..971)
                     /locus_tag="VN039_06530"
     CDS             complement(33..971)
                     /locus_tag="VN039_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45663.1"
                     /translation="MSVETEDAFQKELVELFVQEAQEWLQQIHVALDELQQAPPFERH
                     RVLAQTLKVGITNLGGSAATLNLSDVERASFAALPFVEAVQDPSAQISADDFIALCKQ
                     LGHVHRTLTRATGVAFDEDHAPAPDEDPPPMIATKALLALLRELQDQRTASNPFHRNV
                     VQTMLVQAEGLMERGVEQCNVASIQEFLDRSAEGDQGFLQVVQQELPRLTAVLQTLKN
                     DGVVPGPPSSDLHNAAERVAQLWSAAQQVNASHATVFFMGLHSFLTVVLQHRMVVSAK
                     NYDAIQSRLAQSAKGIEEWAESGRMERMAIQSVLPH"
     gene            complement(1026..2216)
                     /locus_tag="VN039_06535"
     CDS             complement(1026..2216)
                     /locus_tag="VN039_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45664.1"
                     /translation="MPKILIADDSIAVRKVAERLLTEAGLVVTLAANGEEALAYLAKE
                     RPDVVVSDVIMPDKSGYEVCAFIRGNATLAATPVLLISGIVNDEVTRQAESCRADGVL
                     KKPFQGTSLKDRVLELIAKRQEPESAAVDGPASVPAATPADKMMRVSDQPSLGLSQEA
                     GKLKEVEEQLREERGRCKELSTRLSESTEQLARAKESEAQLATERQRADDLQETLAKV
                     EQQASRIPELESALKTEQEAVDAFKQEAANWQKASGRIAELESALHAERAAAEQLVQQ
                     LAELEQVAIRSRDMEARLASAEQQGVELHQKLQDLEAELLTERRKGEKSIARLHELEQ
                     AATRVQEMERLLTAEQDRNGMLARRVMEAEQGAENATKRLEEMARKLSEIAGLASQLG
                     HGKG"
     gene            complement(2256..>2445)
                     /locus_tag="VN039_06540"
     CDS             complement(2256..>2445)
                     /locus_tag="VN039_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45665.1"
                     /translation="LRVKHPLGEMAICIDEEIPVLHTFDPAAIQTYHGKDLPAEGSYT
                     NGLDEIPILSVPRLGSTV"
BASE COUNT          483 a          704 c          632 g          626 t
ORIGIN      
        1 cggtcagatc gagcttcggg tacgggactt attcaatgag gcaatacgct ttgtatagcc
       61 atgcgctcca tccgtccgct ttccgcccac tcctcaattc ccttcgcgct ctgggccaga
      121 cgtgattgga tcgcgtcata gtttttggca ctcactacca tacggtgctg cagcacaacc
      181 gtaagaaaac tatgcaatcc cataaaaaac accgtcgcat gggaggcatt gacctgctga
      241 gcggcagacc acagctgcgc aacacgttca gcagcattat gcaggtcgga ggatggtggg
      301 ccggggacga cgccatcgtt cttaagagtt tgaagaacag ccgtcagacg aggaagctct
      361 tgttgaacga cttggagaaa accttgatct ccttcggctg accgatcgag aaactcctgg
      421 atggaagcga cattgcattg ttcgaccccg cgctccatca acccctccgc ctggaccagc
      481 atcgtctgaa cgacattgcg atggaatggg ttggatgccg tacgttgatc ttgaagttca
      541 cggagtaacg ctaaaagcgc tttcgtcgcg atcatcggtg gtgggtcttc gtcgggagca
      601 ggtgcatggt cctcatcaaa tgccacacct gtcgctctag tcagagttct atgaacgtgc
      661 cccagctgct tgcagagtgc gatgaagtca tcggcggaaa tttgcgcaga aggatcttga
      721 acggcttcaa caaatgggag agctgcaaaa ctagcccgtt cgacatcgct caggttgagc
      781 gtggctgcgg atccgccgag gttcgtaatg ccgaccttga gcgtttgggc caagacacga
      841 tggcgttcaa aagggggagc ttgctgaagt tcatcgagag cgacgtgaat ttgctggagc
      901 cattcctgtg cctcttgaac gaacagctca accagctctt tctggaacgc atcttcggtt
      961 tcaacggaca tcggcttctg cccttcaact agaccatcga ggtgctcaga ttgaccgcgt
     1021 caggattatc cctttccatg tccaagttgg gaggccaatc ctgcaatttc acttaatttg
     1081 cgtgccattt cctcaagccg tttggtggcg ttctcggcgc cttgttcggc ttccattact
     1141 cgtcgagcga gcattccgtt gcgatcctgt tccgcagtca ggagccgttc catttcttga
     1201 actctcgtcg ccgcttgctc caattcgtga agtcgcgcta tgctcttctc accctttcgc
     1261 cgttccgtca aaagttcggc ttcgaggtcc tgaagttttt ggtgaagttc gactccctgc
     1321 tgttccgcac tcgcgagcct tgcttccatg tctcttgatc tgattgccac ctgctccaac
     1381 tccgccagtt gttgcacgag ttgttctgcc gccgcccgtt cagcgtgcag agcggactcc
     1441 agttctgcaa ttcgaccgga agccttttgc cagtttgcgg cttcctgttt gaatgcatcg
     1501 actgcttcct gttccgtctt cagtgccgat tccaactcag ggattcttga cgcttgttgc
     1561 tctaccttgg cgagtgtctc ctgaagatcg tcggcacgtt ggcgctcggt tgccaactgt
     1621 gcttcgcttt ccttggctct ggcgagctgc tccgtagact cagacagtcg cgtgcttagt
     1681 tctttacagc gacctcgttc ctcccgtaac tgttcttcaa cctctttcag tttgccggcc
     1741 tcctggctca gacccagtga cggttggtct gataccctca tcattttgtc ggcgggagtc
     1801 gccgcaggaa cggaagcagg tccgtcgacc gcggccgatt ccggttcctg ccgttttgct
     1861 atcagctcga ggactcgatc ctttagggac gtaccttgaa acggcttctt caataccccg
     1921 tccgcgcggc aggactcggc ctgtctggtc acttcatcgt tcacgattcc ggaaatgagc
     1981 agcacgggcg tcgctgcaag ggttgcgttt ccacggataa atgcacagac ttcatatcca
     2041 ctcttgtccg gcatgatgac gtcggacaca acaacatcgg gccgttcttt ggccagatag
     2101 gccaatgctt cctctccgtt tgccgcgaga gtgacgacga ggccggcctc ggtcaatagt
     2161 cgctcggcaa ccttgcgaac cgcgatgctg tcatcagcta ttaagatctt cggcatgcct
     2221 gcacctttcg tccatgctga cacaaccggg aatcgtcaca cagtcgaccc aagtcgtggt
     2281 acagagagaa tcgggatctc gtccaggcca ttggtataac tgccttcggc cggcaaatct
     2341 tttccatggt acgtctgaat cgcggcagga tcaaaggtat gaagaaccgg aatctcttca
     2401 tcaatacata tcgccatttc tccgagagga tgcttcactc tcaga
//
LOCUS       DATMTI010000399         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_312756, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000399 DATMTI010000000
VERSION     DATMTI010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312756"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(489..791)
                     /locus_tag="VN039_06545"
     CDS             complement(489..791)
                     /locus_tag="VN039_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45666.1"
                     /translation="MSSRVSRTYQVHNLVYFEQHEDMRQAVQREKQIKKWNRAWKIEF
                     NREDQLNMAGLMAEHLLTTWIPAVDHGPSRHSRMLQAGIQKVRGLDARFRVHDTLS"
     gene            complement(805..912)
                     /locus_tag="VN039_06550"
     CDS             complement(805..912)
                     /locus_tag="VN039_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWV45667.1"
                     /translation="MGIDTVTKQPAVYILASRRNGTLYVGVTSNLVKRV"
     gene            complement(1089..1994)
                     /locus_tag="VN039_06555"
     CDS             complement(1089..1994)
                     /locus_tag="VN039_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45668.1"
                     /translation="MKTRISAVICLLCVLGFSDLTRAEDPYSQRVADPYALSPDPPTM
                     SSGTPELYLSFMAGVALPLSEDATFTDGTTPTVIPKVDYRNNYSIGGNAGIWFPTRNK
                     LAGFDLGIELTGFVWYPDVTCCRDFFNNDPTGSKNSQGIANNGTTTEMQGIYIGPNFL
                     LRYPMAISDAYPNGRWFPYAGIGVGMHQMSFRPGGDRGVTSCCIPGGNLNTNPQTDQR
                     DTTVGFMGIGGVKAHLFKYVAAFVEAKYLHAHHNGLGSDRFGNSSCCSLGVPTGNDVV
                     LNTYSSSINTIFVHAGLSIHFDMKP"
     gene            complement(2383..3255)
                     /gene="sucD"
                     /locus_tag="VN039_06560"
     CDS             complement(2383..3255)
                     /gene="sucD"
                     /locus_tag="VN039_06560"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWV45669.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTKVVAGVTPGKA
                     GQEVEGIPVFNTVADAVKKTECDTSLIFVPPPFCADAILEAADAGVKLIICITEGIPV
                     NDMVKVKRALRGREVRLIGPNCPGVITVDEAKIGIMPGFIHKKGVVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFEKDPETQAIVMIGEIGGDAEEKA
                     ADFIKKNVKKPVISFIAGITAPPGRRMGHAGAIISGGKGTAAEKMKTLEAAGVKVVKN
                     PAEIGAAVKQALGR"
BASE COUNT          768 a          991 c          890 g          738 t
ORIGIN      
        1 tgagatcgga gtagcccaag acacagagca ggcaaacaat cgccgacatt ctcgtgttca
       61 ttcatcgccc tcccacggta cagacaccat gttcttcgac gaattggggt cgcgcaatct
      121 gagatccgtc agtctctcgt cgatggtcag tgaggcgaga tgagacgcgt acatctgtgt
      181 gcgactcgaa tcgccctccg gttcggctgt tgcggttatt ggcataagcg tccatctctc
      241 cgcctccaga gcgatcagga ggggatactc ttcttgcacc gccggataag gacggcgccc
      301 acagtagcaa tcccacgttc agttttgaaa atagataaaa cggattggag ctatcggtaa
      361 atccaatacc ccaggatgaa cagagcaccg tactccctaa cccgcattat ccaggatgcc
      421 gcagctgagc agttgccatc acgagggagc gattggactc cctggcaaga cggaaagatg
      481 gcgcggcctc aagaaagagt gtcatgcacg cgaaagcggg catccagtcc ccggactttc
      541 tggattcccg cttgaagcat gcgggaatga cgagaagggc cgtggtcaac tgcgggaatc
      601 caggtagtca gcaaatgctc ggccataagt cctgccatgt tgagttggtc ttctcgattg
      661 aactcaattt tccatgcacg attccacttc ttgatttgct tctcgcgctg gacggcttgg
      721 cgcatgtcct catgctgctc aaagtacaca agattgtgca cctggtacgt ccgggaaacc
      781 cgcgacgaca ttctgtttat gttcctacac acgcttgacc aggttcgagg tcacaccgac
      841 atacagcgtc ccgtttcgcc tactcgcgag aatgtagact gcaggctgtt ttgtcacggt
      901 atcaataccc atggattccc gcgccccgct taaaacatgc gggcacaagc ttcgcggaat
      961 gacggagcgt cgagaacagt gggacgccct gctcgatcag tcgcgcgtct cggcgtcgga
     1021 tgagaagaac aagcagcgcc gctaccccac cgattcgcag tgggatagcg gtgagacact
     1081 gaatgcggtt acggcttcat gtcaaagtga atcgacaagc ccgcatgtac aaagatcgta
     1141 ttgatcgagg atgaataggt attaagaact acatcgttcc ccgtcggcac gcctaatgag
     1201 cagcatgagg aattcccgaa ccgatccgac cccagcccgt tgtgatgggc atggaggtac
     1261 ttcgcctcca caaacgccgc cacgtacttg aagagatgcg ccttgactcc tcctattccc
     1321 ataaacccga ccgtcgtatc ccgttggtcg gtctgggggt tcgtattcaa attaccccca
     1381 ggaatacaac acgaagtaac tccgcggtct ccaccgggcc taaatgacat ctgatgcatg
     1441 cccacgccga tcccggcgta cggaaaccac cgcccgttgg gataggcgtc ggaaattgcc
     1501 atcggatagc gaagcaggaa attggggccg atgtagatgc cttgcatttc agtcgtggtg
     1561 ccgttattgg cgatcccttg actattctta ctccccgtgg ggtcattatt aaaaaagtcc
     1621 cgacagcagg tcacgtccgg ataccacaca aagccggtaa gttcaatgcc gagatcgaat
     1681 cccgccaact tgtttctggt tggaaaccag attcccgcgt tgcccccgat cgaatagtta
     1741 ttcctatagt ccaccttcgg gatcaccgta ggcgttgtcc cgtccgtaaa cgtggcgtcc
     1801 tcactgagcg gcagcgccac gcccgccata aaagaaagat agagctcggg cgtcccggag
     1861 gacatggtcg ggggatcagg cgagagggca tagggatcgg cgacccgttg gctgtacgga
     1921 tcttccgccc tggtgagatc cgaaaagccg aggacacaga ggagacaaat caccgccgaa
     1981 attctcgttt tcattcatcg tcctcccacg gtaaagaaac catgttcttc gacgaatttg
     2041 ggtcgcgaaa tctgctggcc atctgtcgat cgtcgatgat caatgcggcg agacgagacg
     2101 agtgcgcctt cgtgagactc gaatcgaccc ccggttcggc tggtgcggtt cttgacataa
     2161 gagtccaccg atccacctcc agagcgatca ggaggggata ctcttctcgc atcgtagggg
     2221 cggacggcgc ccacagtagc aaccccgcgt tcagttttga aaatagataa aacggattgg
     2281 agctatcggt aaatccaata ccccatcgga caggactgtg gcgatgacac cgacgaagtt
     2341 gtgacagaga atgatgccga ccggaagggc aacctaggag tactaccgtc ccaacgcttg
     2401 tttgaccgcc gcaccgatct cagctggatt cttgacgact ttgaccccgg ctgcctccag
     2461 ggttttcatc ttttcggctg cggtcccctt gccgccggag ataatggcac cggcgtggcc
     2521 catgcggcgt cctggaggcg ccgtgatgcc ggcgatgaag ctgatcaccg gcttcttcac
     2581 atttttcttt atgaagtccg ccgctttttc ctcggcgtcg ccgccgattt caccgatcat
     2641 gacgatcgcc tgtgtttcgg gatctttctc gaacaacggc aggacatcga caaaacccgt
     2701 tccattaacc ggatctccgc cgatcccgac gcaagtcgtc tcgcctaacc ccaaagtcga
     2761 gagttgatgg acggcttcat acgtgagggt gccgctgcga gagaccacgc cgaccacacc
     2821 cttcttgtga atgaagcccg gcatgatgcc gatcttcgct tcgtccaccg tgatgacgcc
     2881 tgggcaattc ggtccgatca accgcacctc gcgcccacga agggcccgtt ttactttcac
     2941 catgtcgttg acaggaatcc cctcggtaat gcagatgatc aatttcacac cggcatctgc
     3001 cgcttcgaga atcgcgtcag cgcaaaacgg cggcggcacg aagatgagtg aggtatcgca
     3061 ctcggttttc ttcaccgcat cagccaccgt gttgaagacg ggaatgcctt cgacttcctg
     3121 cccggccttg cccggggtga ccccggccac caccttcgtc ccataggcct tgcattgcgt
     3181 cgcatggaac gagccttcct tgcccgtaat tccttgcacc accacccgcg tatttttatt
     3241 gacgagaatg ctcatgacga tcctttctac agcagtcaat acacttcttc gtgagtgccg
     3301 acttcaatca agagaatgtc atcctctttg ccggagcctt tgacaaactc aaaaacgaga
     3361 cgcagatcat agccgatgct acaggcc
//
LOCUS       DATMTI010000400         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_439516, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000400 DATMTI010000000
VERSION     DATMTI010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439516"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VN039_06565"
     CDS             <1..1327
                     /locus_tag="VN039_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HWV45670.1"
                     /translation="GATKPLISLAHFSFHVPLHGLFLKPTHVGTVHVSGMAINIPPRQ
                     MRQQASGPKRKLGKIKIVVDEIVVDDSRLVIGTNKPNKDPKVFELKQIVMHNVGPNHP
                     WKYDATLVNAVPRGDIHAVGTFGPWNTESPGDSSVTGHYTFDHADLNTIKGISGMLSS
                     VGDFKGQLDRIEVEGTTETPDFALDTANHPMPLHTKYHAIVDGTSGDTYLQPVEAKLG
                     ESEFSCSGAIVNVKGKGHIIDLDVDVPNGRIQDFLQLAVKTEPAVMTGQVAIKAKLHI
                     RPGKESVSQKLGLKGAFTLHRIHFTNPEVEDKVDMLSLRAQGDPKEAKPGAADVHSQM
                     QGKFVMGDGRLRFSNLLYELPGGTVQLSGVYSLDGKEFNFTGTVRTKAKLSQMVASKW
                     KSWLLKPVDPFFSKNGAGTQVPIKVTGTKGAPKFGLNFGHKDKAGKDKP"
     gene            complement(1338..>1604)
                     /locus_tag="VN039_06570"
     CDS             complement(1338..>1604)
                     /locus_tag="VN039_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45671.1"
                     /translation="DKRGRVMSYYTMAFVGMAPFGSLLAGYLAHRVGAPHAVMITGTF
                     CIIGAAWFTTQLKSIRRIMRPIYIDMGIMQNPMEPAMEDHAGNN"
BASE COUNT          385 a          393 c          504 g          322 t
ORIGIN      
        1 aggcgcgacg aagccgctca tctcgctggc gcacttttca tttcatgttc cgttacatgg
       61 actttttttg aagccgaccc atgtcggcac cgtccatgtg agcggaatgg cgatcaatat
      121 tcctccgcgt cagatgcggc agcaggccag tggaccgaag cgaaagctgg gcaagatcaa
      181 aattgttgtc gacgagattg tcgtcgatga ttcgcggctg gtgatcggaa cgaacaagcc
      241 gaataaagat ccgaaggtct ttgaactgaa gcagattgtg atgcacaacg taggcccgaa
      301 ccatccctgg aagtacgatg caaccctggt gaacgcggtg cccaggggcg acattcacgc
      361 ggttggaacc ttcggcccgt ggaacacgga gagtcccggc gattcctcgg tgaccggaca
      421 ctatacgttc gatcatgccg acctgaatac gatcaagggc atcagcggaa tgctctcatc
      481 cgtaggcgat ttcaaagggc agctggaccg cattgaagtc gagggaacga cggagactcc
      541 cgatttcgca ctggatacgg cgaatcatcc gatgcctctg catacgaagt atcacgcgat
      601 tgtggatggc accagcggtg acacctatct gcaaccagtc gaggccaagc tgggcgagtc
      661 ggagttttcg tgcagcggtg caatcgtgaa cgtgaaaggc aaggggcaca tcatcgatct
      721 cgatgtggat gtgcccaacg gccgcatcca ggacttctta cagcttgccg tgaagacaga
      781 accggcagtg atgacggggc aggtggcgat caaagcgaaa ctgcatattc gtccgggcaa
      841 ggagagcgtc tcgcagaagc tggggctgaa gggagcattt acgttacatc ggattcactt
      901 caccaatccg gaggtcgagg acaaggtgga catgctgagt ctgcgggcgc aaggcgatcc
      961 caaagaggcg aagcccggag cggcggacgt tcattcgcag atgcagggga agtttgtgat
     1021 gggtgacgga agactgcgct tttcaaacct gctctatgag ctgccgggcg gaacagtaca
     1081 gttgagtggc gtgtactcgt tggacgggaa agaatttaac ttcacgggaa ctgtgaggac
     1141 gaaagcaaag ttatcccaga tggtggcctc gaagtggaag tcatggctgc tgaagccggt
     1201 cgatcccttc tttagcaaga atggtgctgg cactcaggtc cctatcaagg tcacaggaac
     1261 aaagggagcg ccgaagttcg gattgaactt tggacataaa gataaggcgg ggaaagataa
     1321 gccttagcgc cttgtagcta gttgttgccg gcgtggtctt ccatcgcagg ctccatcgga
     1381 ttttgcatga tgcccatgtc gatgtaaatg gggcgcatga tccggcggat ggacttcagc
     1441 tgggtggtga accaggccgc tccgatgatg cagaaagtac ctgtaatcat gacagcatga
     1501 ggtgcgccga cacggtgggc aaggtatccg gcaagcaggc tgccgaaggg cgccatgccg
     1561 acgaaggcca tggtgtagta gctcatgacg cggccgcgct tgtc
//
LOCUS       DATMTI010000401         1930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_736683, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000401 DATMTI010000000
VERSION     DATMTI010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736683"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1481)
                     /locus_tag="VN039_06575"
     CDS             complement(63..1481)
                     /locus_tag="VN039_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HWV45672.1"
                     /translation="MAITSGVSLFRSREEILDDMLADLQTLIPDAYVGQDGVIYILFS
                     IFAGVMESLFVNTELLRNDVFVETASEDALDRRGTEKGVPRLPGVQATGNLLFSGDGG
                     VPIDIETEVAYDPGTGADFLYFTTVSAATIPNPGAPTAPDVALNATAGNLTGSFEYVI
                     TFLTAAGETLAGAESAAIVCAAQQVDLSTIPLGGTGTTGRRIYRQKDGSGAYNFVDEI
                     ADNVTTTYTDNIADGALGGNPPAVSTAERVTVPAQAEEAGSDYNVAAGTITILTNAPD
                     GVVAVTNPAPFSGATDPEGFEDFRVRLGEAVKSPQTGAPGDIQVWAEEVDGVESATVF
                     ANDNEGVATNGHTTVRIAGPNGTVPDAGVISAVQTALDTRDLANVTIHVTTFVPTSTD
                     VGVTLTLAAGYTHADVDSSVISAITNYINSLQVGETFRTAGVYSAVFGLAGVTDLTVD
                     TPATNLATGSTAKRTPGTITVS"
     gene            complement(1494..1904)
                     /locus_tag="VN039_06580"
     CDS             complement(1494..1904)
                     /locus_tag="VN039_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45673.1"
                     /translation="MSWDLALEGESGDLIFSPTLDLLGATGDGLTKQRVLIRCRIPRG
                     SWTYDLTGELGSRLATISRNPGPIQLANAPAMVKEALAPMEDITVDDVQATVDENNRL
                     VVSVSYTPVLTQDEADAIGGVSADIPVIDATVTI"
BASE COUNT          418 a          571 c          553 g          388 t
ORIGIN      
        1 gttcgatttc agtgagagcg acttcagccg gaagcgaggg ttgagcgtta gggaacggca
       61 tttcaactca ccgtgatcgt tcccggcgtc cgctttgcag tggagccggt agcgaggttg
      121 gttgcagggg tgtcaaccgt caggtcagtg acaccagcaa gaccgaacac cgccgaatag
      181 acaccggcag tgcggaaggt ctcgccgacc tgaagcgagt tgatgtaatt ggtgatcgct
      241 gaaatcacac ttgagtcaac gtctgcgtgt gtgtaaccag cggcaagcgt cagcgtcacg
      301 ccgacatcgg tcgatgtcgg tacgaaggtg gtcacgtgaa tcgtgacatt cgcaaggtct
      361 cgagtatcca gcgccgtctg aaccgctgaa atcacaccag cgtcagggac ggttccattc
      421 gggccagcga tgcgaacggt cgtgtggccg ttggtcgcaa caccttcgtt gtcgtttgca
      481 aagaccgtgg ccgactctac gccatccact tcctctgccc acacttggat gtcccccggt
      541 gcgccggtct gaggactctt cacagcctct ccaagcctca ccctgaagtc ctcgaacccc
      601 tcggggtctg tagcccctga gaacggcgca ggattcgtca cagccaccac gccatcggga
      661 gcattcgtga gaatcgtgat cgtaccggca gcgacgttgt aatcactacc agcttcttca
      721 gcctgggcag gaaccgtgac gcgctccgcg gtcgagacag ccgggggatt tccaccaagg
      781 gcaccgtcag cgatgttgtc agtgtaggtg gtcgtcacgt tgtcggcaat ttcatccacg
      841 aaattgtagg cacccgaccc atccttctgc cggtagatgc ggcgacccgt tgtgccggtg
      901 ccaccgagag ggatggtgga aagatcgacc tgttgagcgg cgcagacgat ggcagcggat
      961 tcagcacccg ccagcgtttc accggccgcg gtcaggaatg taatcacgta ctcgaacgag
     1021 ccggtgagat tccctgctgt ggcattgagc gcaacatcgg gagcagtcgg tgccccaggg
     1081 ttcgggatcg ttgcggcgct cacggtggtg aagtagagga aatcagcacc agtcccagga
     1141 tcgtaagcaa cctcggtctc tatgtcgatg ggcaccccac cgtcacctga aaacaggaga
     1201 tttcccgtcg cctgaacgcc gggcagtctc ggtacaccct tttcagttcc gcgccggtcg
     1261 agcgcgtctt cacttgcggt ctccacgaag acgtcgtttc gaagaagctc agtgttcacg
     1321 aacagcgact ccatcacacc ggcaaagatt gaaaacaaaa tgtagatgac gccatcctgt
     1381 ccgacgtagg catccgggat gagtgtctga agatcagcca acatgtcatc caggatttcc
     1441 tcgcgagacc tgaagagcga cacacctgaa gtaattgcca tttcaccgcc ctcctagatc
     1501 gtcacggtcg cgtcgattac agggatgtcg gcgctcacgc cgccaatggc atcagcctcg
     1561 tcctgtgtaa gaacaggcgt gtaggaaacc gaaaccacaa gtctgttgtt ttcatcaacc
     1621 gtcgcctgaa catcatcgac ggtgatgtct tccatcggtg ccagtgcttc cttgaccatc
     1681 gcaggagcgt tggcaagctg gatcggccca gggttccgtg aaatcgtggc aaggcgagac
     1741 ccaagctcgc cggtcaggtc atacgtccac gaaccacgag gaatgcggca tcgaatgaga
     1801 acccgctgct ttgtcaagcc gtccccggtg gcaccgagaa ggtcaagtgt ggggctgaag
     1861 atcagatcgc cactctcgcc ttcaagcgcc aagtcccaac tcatagctct tcaccccctg
     1921 cacgaacata
//
LOCUS       DATMTI010000402         1834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_228938, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000402 DATMTI010000000
VERSION     DATMTI010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228938"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VN039_06585"
     CDS             <1..604
                     /locus_tag="VN039_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45674.1"
                     /translation="IDYAERFDAVPFQFHGLEKELSKNVPLALSNGLSWFVNDRKLFR
                     FRGGRLLSNVFPNCSTEFATTLADLVRGGSDMEAEFALATLQNYNGVISAHVVLKEIV
                     ARFPDDQRKLNEVRVCIDNTGVVRGEFGFAEALQARKESMAEWLRDERPAVQAFARKH
                     ISELDRMIALERQRAESRREMRQRDYEEEDGGSDEGEISN"
     gene            complement(1137..>1834)
                     /gene="nth"
                     /locus_tag="VN039_06590"
     CDS             complement(1137..>1834)
                     /gene="nth"
                     /locus_tag="VN039_06590"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWV45675.1"
                     /translation="SLLIQPLIVRVQKQPFEITTAFRKIRKTIKPFPKAAMFQLADEG
                     FISTFEQLVACLISVRTRDETTVPTARRLFSIARTPAHVAKLSVERIEELIHGTSFRE
                     VKAGHIREIAIRTVQNYGGMLPCEEAVLLSFAGVGPKCAHLVLGIACGKSAISVDTHV
                     HRVTNRWGFISASTPEQTMRHLETKLPKRLWIEINRLLVPFGKHICTPTLPKCSACPV
                     RNMCRQVGVTKHR"
BASE COUNT          440 a          460 c          485 g          449 t
ORIGIN      
        1 aatcgactat gcagagcgtt tcgatgccgt accgtttcag ttccatggtc tggagaaaga
       61 gctctcgaag aatgtgcccc tagcactcag caacggacta tcgtggttcg ttaatgatcg
      121 aaaacttttc cggttccgag gggggcgcct gcttagcaat gtattcccca actgtagtac
      181 ggagttcgct acgacccttg ccgatttggt aagaggcggc agtgatatgg aggcagaatt
      241 tgcactggcg actctccaga actataacgg tgtgatatcg gcccatgttg tgttgaagga
      301 aattgtggcg cggtttccgg atgaccagcg caaattgaac gaggtccggg tctgtatcga
      361 caatactggt gtagtcaggg gagaatttgg ctttgcagag gcattgcaag ccaggaaaga
      421 atccatggcg gaatggctgc gagacgaacg gcctgcggtc caagcgttcg cccgtaaaca
      481 tatatcagaa ctcgaccgca tgattgcatt agaacggcaa cgcgctgagt cgagaaggga
      541 aatgcgacag agggattatg aggaggagga cgggggatca gacgaaggcg agatcagcaa
      601 ttgatcggtt ccactgatgt aagatgtgaa gatcacagac attctttcaa ttctacgagc
      661 cgttgatcgg ttgcaaactg acgcaccttg gctcgttcct gtttaaggtg gtattgccct
      721 tccacctcct cttggcatgg cgtcatctcg agaagttcac agcagcgttc cattggtgtc
      781 ttgccgacga gaccgtacca ctcacctcac cgcgttcttg atcagcatcg cattgttcta
      841 attctggggg ggtacgcgat gagtgtaagc ttggttccaa acgcatccaa gccaaattac
      901 tatgccatca tgcgattcgt ttgtccaccg caacgatctg gaaggtcccg catccttggt
      961 tgaggcatag agccgaaccc agatctgtcg gcggcgaagc tcttcgtgat taccacgcac
     1021 ggaacagaac tatacattag ctcattgaaa ggatgtgtgt gatcctgaca ggtgtatcaa
     1081 catcttcgct tttccgtcgc gtgggtcaga tagaccgact tgcttcgcaa cccatttcaa
     1141 cggtgtttgg tgacaccgac ttgccgacac atgttacgta ccgggcatgc ggaacacttc
     1201 ggcaaggtcg gggtgcagat gtgtttacca aatggaacaa ggagacgatt gatctcaatc
     1261 cacaaccttt tcggcaattt ggtctcaagg tgccgcatgg tctgttcggg cgtcgatgct
     1321 gaaataaatc cccatcgatt cgtcacccga tggacatggg tatccacact gatcgcagat
     1381 ttcccgcacg cgatacctaa cacgagatgg gcgcattttg gacctactcc tgcgaaagac
     1441 agtagaacgg cttcctcaca cggcagcatc ccgccataat tttgtaccgt acgtatcgcg
     1501 atttcccgta tatgtccggc tttgacctct cgaaacgacg ttccatgaat cagctcttcg
     1561 atccgttcaa cggaaagctt cgcaacatga gcaggtgtcc tcgctattga gaacaatcgc
     1621 cgggccgtgg ggaccgtggt ttcatcccga gttcggaccg aaatcaaaca cgccaccaat
     1681 tgctcgaacg tcgaaatgaa cccttcatca gccagctgaa acatggccgc ctttggaaat
     1741 ggttttatgg tcttcctaat ctttcggaag gcagtggtga tttcaaacgg ttgtttttgc
     1801 acacgcacga tcagtggttg gatcaacagg ctct
//
LOCUS       DATMTI010000403         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_313042, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000403 DATMTI010000000
VERSION     DATMTI010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313042"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VN039_06595"
     CDS             <1..555
                     /locus_tag="VN039_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45676.1"
                     /translation="KEAPKHIVAITQPGYINDVVFHSISLVYQDCYTGCMASYLTALR
                     AVASVYAKRIVRFAGFVALSFFVVALVLTWFLAAEFSSYWWLALIFILPMGFIGLVAF
                     LLVWRLIAAVYHPSLNATQRRTVGSYVDKLQAVMETAQLSFWFILVMIGKDLLLHRDL
                     RSLEQFIGDSTSLKRDLDDLRTKL"
     gene            complement(550..>1864)
                     /gene="aspS"
                     /locus_tag="VN039_06600"
     CDS             complement(550..>1864)
                     /gene="aspS"
                     /locus_tag="VN039_06600"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWV45677.1"
                     /translation="SEDFTEVTTPILANSSPEGARDFLIPSRLHAGKFYALPQAPQQF
                     KQLLMVGGVPRYYQLAACFRDEDPRADRLYGEFYQLDTEMSFVEDGEVVRGMFDPLIK
                     KLSTEFAGKRLLSEDIPRIPYVEAMEKYGSDKPDLRFGMELVELTDVFVNTEFGVFKS
                     AEAVKAIRVKNGVSLSRKQIDEFTETAKQEGAGGLAYIKYSKNDAGNLTAESPIVKFL
                     NDAELQELENKMDVEDGDVIFFGADKRVVVNKVLGRLRNEFANHFGLKDPNVIALCWI
                     VDFPFYEWDEGRKRVDFGHNPFTMPRGGVEALDVATDDEKLAIVADQYDMVMNGYEIC
                     SGGVRNHSPEVLYKAFGLLGYDEAYVDEKFGAMINAFKYGAPPHAGCAFGVDRMFMEL
                     MDESNIREVIAFPKNGSGVDVMMGSPSVVDQSQLDELSIAVKDSL"
BASE COUNT          420 a          519 c          445 g          480 t
ORIGIN      
        1 aaagaagctc cgaaacacat cgtagccatc acccagcccg gatatataaa tgatgtagtc
       61 tttcatagta tctctttagt ataccaagat tgctatactg gatgtatggc ttcttatctt
      121 accgcactgc gcgcagtcgc atccgtgtac gcgaaacgca tcgtacgttt cgccggcttc
      181 gtggcattga gcttctttgt tgtcgctctt gtccttacgt ggttcttagc agctgagttc
      241 agcagctact ggtggctggc gcttatcttc atcctgccga tgggattcat cggacttgta
      301 gcattcttgt tagtttggcg actgattgct gccgtatacc atccgtcgtt aaatgcgaca
      361 caacgtcgta ccgtcggaag ttatgtcgac aagttgcagg cagtcatgga aactgcccag
      421 ttgtcattct ggttcatcct tgtgatgatc ggcaaagatt tgttacttca ccgtgatctg
      481 cgctcactcg agcaatttat cggtgattcg acatctttaa agcgtgatct cgacgatctg
      541 agaacaaaat tataaggagt ctttgactgc aatcgatagc tcgtcgagct gcgactgatc
      601 aacgacgctc ggtgagccca tcatgacatc cacaccggag ccgttctttg gaaaggcgat
      661 gacctcgcgg atgttactct catccatcag ctccatgaac atccggtcga caccaaacgc
      721 acagccggca tgtggcggcg cgccgtattt gaacgcgtta atcatggcgc cgaacttctc
      781 atcgacgtat gcttcgtcgt aacccaggag accgaaggcc ttatagagta cctcggggct
      841 gtgattacgc acgccaccgc tacagatctc gtaaccgttc atcaccatgt catattgatc
      901 ggccacaatc gccagcttct catcatcggt cgcgacatcc agcgcttcga cgccaccacg
      961 cggcattgtg aacgggttgt ggccaaaatc gacgcgtttc cggccctcat cccactcata
     1021 aaaaggaaag tcgacgatcc agcagagcgc tatcacgttc gggtctttaa ggccgaaatg
     1081 gttcgcgaat tcgttgcgca accggccaag tactttgttg acgaccactc gtttgtcggc
     1141 accgaagaaa ataacgtcgc cgtcttcgac gtccatctta ttctctagtt cctgcaattc
     1201 ggcatcattc aggaatttca cgattggtga ctcggccgtc agatttcctg cgtcgttctt
     1261 ggaatatttg atgtatgcca agccgcccgc accctcttgc ttggcagtct cggtgaattc
     1321 atcgatctgc ttgcggctca gactgacgcc attcttcacg cgaatcgcct tgacagcttc
     1381 ggcgctcttg aacacgccga attcggtatt cacgaataca tcggtcagct cgaccagctc
     1441 cataccaaaa cgtagatccg gtttgtcaga gccgtatttc tccattgctt cgacatacgg
     1501 aatgcgcggg atatcttccg acaggagtct cttgccggca aactcagtcg agagcttctt
     1561 gataagcgga tcgaacatac cgcggacgac ttcgccatct tcgacgaaac tcatttcggt
     1621 atcgagttgg taaaactcgc catacagacg atcggctcgc ggatcttcat cacggaaaca
     1681 tgccgccaac tgataatatc gtggtactcc accgaccatc agcaactgct tgaactgctg
     1741 cggcgcttgc ggtagcgcat agaacttgcc cgcgtggagg cgtgacggaa tcaggaaatc
     1801 tcgggcacct tccggactag agttcgccaa gatcggcgtt gttacctctg tgaaatcctc
     1861 cgac
//
LOCUS       DATMTI010000404         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_736848, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000404 DATMTI010000000
VERSION     DATMTI010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736848"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VN039_06605"
     CDS             complement(<1..446)
                     /locus_tag="VN039_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45678.1"
                     /translation="MMRVRATWLIGFGLVGLLSCSGEQDNALQQTTPTSPALPANVFS
                     RMFPESPPFAPPTDEAREQAKKLGMKGGLLDALDDTPRNNPDNPNMTEGVTFFGQFLD
                     HDLTLAMKAQLLAQTDPGQTTNFRTAKFDLDSVYGGGPDGSPELYT"
     gene            complement(917..1282)
                     /locus_tag="VN039_06610"
     CDS             complement(917..1282)
                     /locus_tag="VN039_06610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV45679.1"
                     /translation="MQFRCSPRKYVRYPANVQTDNGAAEGMLFDLSATGCRMQSNAVL
                     TPGSYLALHIDASETASPLAVEVSIVRWHKDNQFGIEFLRYAQGDRERVTDLVEGREA
                     PAISNHVEHEELTLSTAAA"
     gene            complement(1311..1790)
                     /locus_tag="VN039_06615"
     CDS             complement(1311..1790)
                     /locus_tag="VN039_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45680.1"
                     /translation="MKPKTLLTVLALVFILTGFTYTYNSVQFWGFDGVSRAKEWVSPD
                     GRMSLQAQLPDQSTAIDMWPTSGMTPTEHSLAELTLNRYQWDNPAMERISLSAMVTQS
                     REGAYRIGIERDGTGQYRPLIFCFEATSPGVATCPLKITENGVFVKQGAGYTQIGWN"
BASE COUNT          417 a          546 c          572 g          488 t
ORIGIN      
        1 ctggtataca gttcaggcga cccgtcggga ccccctccgt acacgctgtc aagatcaaac
       61 ttggcggtac gaaaattcgt cgtctgcccg ggatccgttt gcgcgagcag ctgcgccttc
      121 atggcgagtg tgagatcgtg atccagaaac tgtccgaaga aggtgacacc ctcggtcata
      181 ttaggattgt cgggattgtt ccggggagta tcgtccaggg catcgagaag gcctcctttc
      241 atgccgagct ttttagcctg ttctcgggct tcatcggtcg gcggcgcaaa aggtggcgac
      301 tctggaaaca tgcgactgaa gacattggcc ggcaaggcag ggctcgtggg tgtggtctgc
      361 tgcagggcgt tgtcctgttc gccgctacag gataataggc cgacaagacc gaacccgata
      421 agccatgttg ctcgaacacg catcatgctc ctccgagtgc gttactgaac tagctcaatt
      481 ggcacttgat agggtcgtta ctcgccatat acgaaggact cagtaatgtc ttgcagacct
      541 tgatgaccat atcgccatcg ggtttggtgg cgcggacagt aacaattcaa gtcggaatgg
      601 aacaattcct cttccgtagg aggaggccgg cctagccctc aatccgatcg aagccgtccg
      661 gccaaattgc cgatcaccga gcagtacgca gcttcttgac ttggcttctt ggcctgcgcg
      721 aggcgttcag cagcgcggcg cgcgcagcag tcggcacaag ccgcggcgat cctttgccag
      781 accgatggag caatttggac agccgtatcg gaaggtacga ttcgaacgct gtgaagaata
      841 tccgtaaatg tccgtcgcct attaagcgga ctcgatcttc acgaatgtgt tgggaaatga
      901 ttcatgggag aagggcttac gcggcggcgg tactgagagt cagttcttca tgctcaacgt
      961 gattggaaat ggcaggggct tcgcgacctt cgaccagatc ggtgactcgc tcgcgatcgc
     1021 cttgggcgta ccgaagaaac tcgatgccga actggttgtc tttgtgccac cgcacgatgg
     1081 agacctcgac agctagcgga gatgctgttt cggaagcatc gatgtgcagc gccaggtagc
     1141 ttcccggcgt caggacggcg ttgctttgca ttctgcagcc tgttgcggag agatcgaaga
     1201 gcatgccttc tgctgcgccg ttgtcggtct ggacgttggc ggggtaccgc acatattttc
     1261 gtgggctgca tcggaactgc atatcgatct cactggtgat gggtactccc ttagttccaa
     1321 ccgatctgag tgtacccagc tccctgtttc acgaagaccc cattctcagt aatcttcaag
     1381 gggcaggtag ccacgcccgg gctggttgcc tcaaagcaga aaatcagtgg ccggtactgc
     1441 cccgttccgt cacgctcgat accaatgcgg tacgcccctt cccgcgactg ggtgaccatc
     1501 gctgacagac tgatgcgctc catcgcgggg ttatcccact gatatcggtt caacgttaac
     1561 tcagccaacg agtgctcggt cggtgtcatc ccgctcgtcg gccacatgtc gatggcggtg
     1621 ctttggtctg ggagctgtgc ctgaaggctc attcgtccgt caggtgacac ccattccttt
     1681 gctctggaga ctccatcgaa gccccaaaac tgaacgctgt tgtatgtgta tgtaaatcct
     1741 gtcaagatga aaaccagtgc taatacggta agtaatgttt taggtttcat ggttccttta
     1801 cttcgtaatc gtcttgtcaa atctctgcaa tcaagcgaca caagcggcgg ctgcctgggc
     1861 tatctcctcg agtgagttcg ccgagttttt cgccaaggcc tgtatcggca gtgccgagaa
     1921 aaacttgaca ggctagtcag gttgcctttc tctcttcggc tcctctaaac ttcatttgtt
     1981 ccgtgctcgg atcgtgcgtt gaatctctat aggagaatcg aac
//
LOCUS       DATMTI010000405         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_609466, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000405 DATMTI010000000
VERSION     DATMTI010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609466"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1373
                     /locus_tag="VN039_06620"
     CDS             336..1373
                     /locus_tag="VN039_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV45681.1"
                     /translation="MTDFHNIFSIRRRVLLKALGLGTLIGGVGGCDAVGGVFGRMFAV
                     PPRETTYFTPNSKFYIVNYADGAVSASRELNIEQWKLHVKGSVKNPMSLGWRDILNRD
                     SHDQISTLMCIDTLPGGDSIGTATWRGISLKKLLIDCGADTDTARDVVFRGIDGYDDS
                     IPFTRAMQDDVMLAFLMNGEKLPREHGFPLRLLVPGLYGIKNVKWIVEIEVYPGDYKG
                     YWQRKGWTDDGTIKIFSRIDSPGHYQTLRGAEHTFRGIAFGGPNSIGKVEISFDAGRT
                     WNDCRIEPTMSPYSWVIWTYTWRPPKPGKFQTVVRATDTKGQLQIAEIVRPQPAGASG
                     YHTIISEVTEL"
     gene            1624..2448
                     /locus_tag="VN039_06625"
     CDS             1624..2448
                     /locus_tag="VN039_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45682.1"
                     /translation="MTDPPLQNPLARFDSQQRPLEISTVQAADDILKQADALTRHDSH
                     ADDMQAAHYYLTAAHFLENQDPLRSVHAYHNAGQQLHWLEQFTQAGRTFSNAGRVAEQ
                     AAAAMPPGPAQCHLQHLAVRAYSRANNSFAEAGELNASEAEYLNERNARLVWSKMQGK
                     HPLALSTWKATSNFGTSIPRWIAWIGGVLAVFSLLYELFFRLAWLAPMGNTTPSAWIP
                     LWSGFYYAINVTSSLALVEYQPTHPICQAVVIFSVIVGYLFLGIGIGIVGRMMQTR"
BASE COUNT          589 a          715 c          630 g          539 t
ORIGIN      
        1 gttcgttctg agccaggatc aaactctcaa ataggtgttc ttagaatttg gaccaagggc
       61 caccacttca atacacctta caccttgctc aacgtcctct attcagtttt caaagaacga
      121 tcacgttcat cacgcgagcc gcgcactata caatgtgcag cgtagcgtgt caagggaccc
      181 gctcgaaata aattcgggta gtttcccttt ggcaccgcac cgtgactcgc gtcacgcgcg
      241 gtcatcaggc aggaacacag taccaacgaa caagcgggaa gataacaagg tattccgcaa
      301 gagtcaagag tccggtcagt gtataatcag aagagatgac agactttcat aacatatttt
      361 cgatccgtcg ccgcgtactc ctgaaggcgt taggacttgg aacgttgatc ggcggcgtcg
      421 gcgggtgcga tgccgttgga ggcgtgttcg gccgtatgtt cgccgtccct ccgcgtgaga
      481 caacctactt cacgcccaac tcaaaattct acatagtgaa ctacgccgat ggagccgtct
      541 ccgcatcgcg cgaactgaac atcgagcagt ggaagctcca tgtcaaaggc tcggtaaaaa
      601 acccgatgtc gcttggttgg cgcgatattt tgaaccgcga ttcccacgat cagatttcca
      661 ccttgatgtg catcgataca ctgcccggtg gagacagcat cggcaccgcc acatggagag
      721 gtatttcact taagaaactg ctcatcgact gcggagcgga caccgacacc gcacgcgatg
      781 tcgtctttcg cggcattgac ggctacgacg acagcattcc gttcacacgc gcgatgcagg
      841 atgacgtaat gctggcattt ctgatgaacg gcgagaaact acccagggaa cacggattcc
      901 cgctccgcct tttggtgcca ggtctctacg gcatcaagaa cgtgaaatgg atcgtcgaga
      961 tcgaagtcta tcccggcgac tacaagggct actggcagcg caagggctgg accgacgacg
     1021 gcaccatcaa gattttctca cgcatcgatt cgcccggaca ttaccaaacg ctgcgcggag
     1081 cggaacatac gtttcgaggc atcgccttcg gcggaccaaa cagtataggc aaagtagaaa
     1141 tcagcttcga tgcaggccgc acctggaacg actgcaggat cgaaccgact atgtcgccct
     1201 attcttgggt gatttggacg tacacctggc ggccgccaaa gccggggaag tttcaaaccg
     1261 tcgtccgcgc caccgacaca aagggacaac ttcaaatcgc cgagattgtg cgcccgcagc
     1321 cagccggtgc cagcgggtat catacgatta tttcggaagt gacagaactc tgaaacaggc
     1381 caccagcgtc gctctcggtc gtttatccac cccaacgtct tcgcttccta cgcttcggta
     1441 cacacaccct agctaaacaa cttgcttgag atttctgtgt gagatgaacc atcagcaaaa
     1501 cacactttgc caagccacca tgccgtctgc actccacaaa ctggaatcaa agtcaccgta
     1561 tgtactgtga atcttccttg ctgacctgaa attggagtac catggcgccc ttggaggata
     1621 ctgatgactg atccgccgct tcaaaatccc cttgcgcgat tcgattctca gcagcgccca
     1681 cttgagattt cgacggttca agcggctgac gacatcctta agcaagccga cgcccttact
     1741 cggcacgact cgcacgcaga tgacatgcag gcagcccatt actatctcac tgccgcccat
     1801 tttctggaaa accaagaccc tctcaggtcg gtacatgcct accacaatgc tggtcagcaa
     1861 ctccattggc tagagcaatt tacgcaagcc gggcgaactt tttccaatgc cgggagggtg
     1921 gcggagcaag cggcagcggc gatgccccct gggccggccc aatgtcacct gcaacatctc
     1981 gccgtgcggg cctattcgcg ggccaataat tcgttcgccg aagccggaga gttgaacgca
     2041 tcagaagcgg aatatctgaa cgagcgaaac gcgcgacttg tctggtctaa gatgcagggt
     2101 aaacatccgc tggctctctc gacatggaag gcaacaagca atttcggcac gagcatcccg
     2161 cgctggatag catggatcgg tggagtactc gcagtcttca gcttgctgta tgagctgttc
     2221 ttccgtcttg cttggctcgc cccgatgggc aatacgacgc cctcagcctg gattcccttg
     2281 tggtccggat tttattacgc aatcaacgtg acttcttcgc tggcgctcgt ggaataccaa
     2341 ccgacacatc ccatttgcca ggctgtggtc atattcagcg taatcgtcgg ctacctcttc
     2401 ctcggtatcg gcattggtat agtagggcgc atgatgcaga cgcgctagtt ttctgatttt
     2461 cccgcgataa cta
//
LOCUS       DATMTI010000406         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_779298, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000406 DATMTI010000000
VERSION     DATMTI010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779298"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /gene="secG"
                     /locus_tag="VN039_06630"
     CDS             complement(<1..110)
                     /gene="secG"
                     /locus_tag="VN039_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWV45683.1"
                     /translation="MLYTLIVVIHVFICFLMIGAILLQSGKGAEIGAAFGG"
     gene            complement(117..899)
                     /gene="tpiA"
                     /locus_tag="VN039_06635"
     CDS             complement(117..899)
                     /gene="tpiA"
                     /locus_tag="VN039_06635"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWV45684.1"
                     /translation="MRTVLIVGNWKMNKTASEAVAFVRELKERLPTPSATVELVVAPP
                     FTALESVRIALGPSSPIQLGAQNMFWEESGAYTGEVSAPMLKDLGCRYVILGHSERRT
                     LFGEQSDGIQKKIRAALEHELRPILCIGESLAQRDNGSTDEVLTQQLRESLSGFTGEA
                     MTGVTVAYEPIWAIGTGKSATTEQAVAAHRTIRQVLASTASPAIADRMRILYGGSVTP
                     ENIESLLTSDQIDGALIGGACLQVESFATIAKVASAARSIGV"
     gene            complement(945..>1695)
                     /gene="pgk"
                     /locus_tag="VN039_06640"
     CDS             complement(945..>1695)
                     /gene="pgk"
                     /locus_tag="VN039_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWV45685.1"
                     /translation="AAHRAHASTVGITRYIKDAAAGALLKKEIEYLEGAVANPVRPFA
                     AILGGAKVSGKIGVIENLGKKVDKVIIGGGMAFTFLKAKGMEIGNSLCEMDMLDFARG
                     IEDHALSRGVKFYLPVDCVVATSREVGAETKIVPVQEIPKGWYALDIGPASVKLFNEA
                     VQNAKTILWNGPMGVFEIDAYARGTLAMAHAIADAYALTIVGGGETSLAVHRAGVSEN
                     MSFISTGGGAALELLEGKTLPGLAALPDRLN"
BASE COUNT          354 a          480 c          483 g          378 t
ORIGIN      
        1 ccgccgaaag cggcgccgat ttccgcgccc ttccctgatt ggagaagaat cgccccaatc
       61 atcagaaagc aaataaagac atggataaca acaatcaacg tatatagcat gaaatcttag
      121 actccgattg atcgggcagc ggaagcgacc tttgcaattg tagcaaagga ctcgacttgg
      181 agacaagcgc cgccgataag tgcgccgtcg atctggtccg aggtaagtag cgattctata
      241 ttctcgggtg tgacgctccc gccatagaga atcctcatgc ggtcggcgat cgccggagag
      301 gctgtggagg caaggacttg ccgaatagtc cggtgggcgg cgacggcttg ctcggttgtt
      361 gccgacttac ctgtgccgat ggcccatatc ggttcatagg cgacggtgac accagtcatt
      421 gcctcgccgg taaagccgga caagctctcg cgcagttgtt gcgtgagcac ctcgtcagtt
      481 gaaccgttgt ctcgctgggc gagcgactcc ccgatgcaga ggattggacg gagttcgtgt
      541 tcgagcgccg ctcggatttt cttctggatg ccgtcgctct gttcgccaaa gagggttcgc
      601 cgttccgaat gccccagaat cacatagcga caaccgaggt ctttaagcat cggtgcggag
      661 acttctcccg tataggcgcc gctctcttcc caaaacatat tctgcgcccc gagctgaatc
      721 ggagacgaag ggcccaaggc gatgcggact gattccaatg cggtgaatgg gggagcaacg
      781 accagttcga cggttgcaga cggagtagga aggcgctctt tcagctcacg aacgaaggcg
      841 actgcttcgg acgcggtctt attcattttc cagttgccga cgattaggac tgtacgcacg
      901 gtgcttctgc tttcaggagt tgaggtttcg ttgaggaatc gtgatcagtt taggcggtca
      961 ggcagcgctg cgagaccagg aagcgttttg ccttcgagta attcaagggc cgcgccgcct
     1021 ccggtcgaga tgaacgacat gttctccgag acgccggccc gatgaacggc caatgacgtc
     1081 tcaccgccgc cgacgatcgt gagcgcatac gcatcggcga ttgcgtgagc cattgccaag
     1141 gtgcccctgg cgtaggcgtc gatttcaaac acacccattg gtccgttcca caggatggtt
     1201 ttcgcatttt ggaccgcctc attgaacagc ttgacggagg ccggtccgat atcgagggca
     1261 taccatccct tgggaatctc ctgtaccggg acaatcttgg tttcagcgcc cacctcccgg
     1321 ctggttgcca ccacgcaatc gacaggcagg taaaatttga cccctcgtga gagggcgtgg
     1381 tcttcgattc ctctggcaaa atccaacatg tccatttcgc agagagaatt accgatctcc
     1441 atgcctttcg ccttcaagaa cgtaaacgcc atgccgccgc cgatgatgac tttatcgact
     1501 ttcttaccca ggttttcgat cacaccgatc ttgccggaca ccttggctcc ccctagaatt
     1561 gcggcaaacg gtcgaacggg attggcgacg gcaccttcga ggtattcgat ctctttcttg
     1621 agcagtgcgc ctgccgccgc atcttttatg tatcgggtga tgccgaccgt cgatgcatgg
     1681 gcccggtggg ccgcc
//
LOCUS       DATMTI010000407         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_525139, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000407 DATMTI010000000
VERSION     DATMTI010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525139"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VN039_06645"
     CDS             <1..181
                     /locus_tag="VN039_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45686.1"
                     /translation="KRHPRSRFDQDTYCNKRLELAIGMLRLHRMHGRANDTVIRFTVL
                     QFSSPDRMDSSVNQA"
     gene            complement(205..915)
                     /locus_tag="VN039_06650"
     CDS             complement(205..915)
                     /locus_tag="VN039_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HWV45687.1"
                     /translation="MNEQTEQQETPQPLYVSLILDESGSMQSCKRAAIAGFNQYVKSL
                     QGEPAPTWFTLTLFNARRTEVRHRGVPVGTVPELDVETYRPQDTTPLYDAIGHTLAAA
                     RQEAPNGTKKLCVILTDGLENSSKEFTRKQIYALIKGCEQEGWRFVYLGADHDVWAAG
                     EDLGVAQECRVAFSKDHLAEAFTDVSSMNARYRRGMEGVAEASDAASGKSGEAAARIA
                     FRRAIRPLWRAVFAGFGQ"
     gene            complement(1124..1633)
                     /locus_tag="VN039_06655"
     CDS             complement(1124..1633)
                     /locus_tag="VN039_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWV45688.1"
                     /translation="MNLDDIPGGSLCVVDTNVLLYAEQGVSTQAQRLLRRIERRELIG
                     VVPQPVWQELTHKLMLAEAMMLGQISGGNPARQLAAKPDVIKRLTTYREKVSALITLG
                     LTFEGCTKTDLLDKALPLQERYGLMTNDSVIAAVAIRLEADALVSADTRFKVVKNLHV
                     YAPSDVKMA"
     gene            complement(1630..1872)
                     /locus_tag="VN039_06660"
     CDS             complement(1630..1872)
                     /locus_tag="VN039_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45689.1"
                     /translation="MKLKIGTQIEEELMKLAKRKAAEEGRSISDLIQDALVQYLSSGA
                     ASPKEREMAYQSFCERPLRLKPEEFRQVLEEDTWDR"
BASE COUNT          396 a          581 c          533 g          436 t
ORIGIN      
        1 gaaacgtcac cctcgctcgc gcttcgatca agacacgtat tgcaacaagc ggctagaatt
       61 agcaattggg atgctgagac tgcatagaat gcacgggcga gccaacgaca ccgtcattcg
      121 atttaccgtc cttcaatttt ccagcccgga ccgtatggat tcgtcggtga atcaggcttg
      181 aaccggctgc ccgcgccatg cgggttattg accgaatcct gcgaaaacgg cgcgccatag
      241 cggccgaatc gccctcctga atgctattcg ggctgctgct tctccggatt taccggacgc
      301 ggcgtcggac gcttccgcca ccccttccat ccctctccga taccgcgcat tcatcgacga
      361 cacatcggtg aaggcctccg ccaggtggtc cttcgagaac gcgacgcggc attcctgggc
      421 gacacccagg tcttcgccgg ctgcccagac gtcgtggtcg gcgcccagat acacaaacct
      481 ccagccttcc tgctcgcagc ctttaatcag ggcatagatc tgctttcgcg tgaattcctt
      541 ggaggagttc tccagcccat cggtgagaat cacacacagc ttcttggtgc catttggcgc
      601 ttcctgtcgc gcggccgcca gggtgtggcc gatggcgtcg tagagcgggg tcgtgtcctg
      661 gggccggtag gtttcgacgt ccaactccgg cacagtaccg accggcacgc cgcggtggcg
      721 gacctccgtt cggcgggcat taaacaacgt gagcgtgaac caggtcggcg ccggctcgcc
      781 ctggagcgac ttcacgtact gattgaagcc cgcgatcgcc gcccgcttgc acgattgcat
      841 cgacccgctc tcatcgagga tcaaggacac gtataacggc tgaggagtct cctgttgctc
      901 ggtctgttcg ttcatggtca cctccgcttg ggttattaga tggccatgat cataatggta
      961 gggcgtgaca gggctggtcc caaagacaac gacaaggacg aatgactaag acctctggat
     1021 aaaacgcgac ttagaaagaa cggcagggga gaaccaatag gaaaggtgtg acatccaagg
     1081 tcactgcgcg agcagccttc gcgcatcttc tttgacggcg ttcttaagcc atcttcacat
     1141 ccgagggagc atagacatgc agattcttta cgactttgaa tcttgtatcc gctgaaacca
     1201 aggcatctgc ttcgaggcgg atggctaccg cagcgataac cgagtcgttg gtcatcagac
     1261 catagcgctc ttgtaatggc agggccttgt cgagcaagtc ggttttggta cagccctcga
     1321 acgttaatcc caatgtgatg agagcggaca ccttctcgcg ataggtcgtg aggcgcttga
     1381 tcacatcggg cttggccgcg agctgtctcg caggattccc acctgaaatc tgtcctaaca
     1441 tcatcgcttc agccaacatg agtttgtgag tcaactcttg ccataccggc tgcgggacca
     1501 cgccaatcag ttcccgacgc tcgattcgtc gcagcagccg ttgagcttgt gttgacaccc
     1561 cttgctcagc gtagagcaac acgttcgtgt cgacgacgca caggctgccg ccgggaatat
     1621 catcgagatt catcgatccc acgtatcttc ttcgagcact tgccggaatt cctcgggctt
     1681 gagcctgagc ggtcgctcac aaaatgactg ataggccatc tcgcgctctt tcgggctcgc
     1741 ggcaccggag ctgaggtatt ggaccaaggc atcctggata agatcactga tggagcgacc
     1801 ctcctctgcg gccttgcgct tagccagctt catgagctct tcttctattt gcgtgccgat
     1861 ttttagtttc atcgggttac tcctttggcc cgagcaccaa tcagggtgtg agcatctcca
     1921 tcacgctgag gccaccatat cggtac
//
LOCUS       DATMTI010000408         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_269744, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000408 DATMTI010000000
VERSION     DATMTI010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269744"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VN039_06665"
     CDS             <1..726
                     /locus_tag="VN039_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV45690.1"
                     /translation="QGVSLRYENQIATALTDVDLTIKPGEVIALVGNSGSGKTTLVGL
                     LPRFYEPTVGVIYIDGIPLTSYELKSLRAHIGIVSQEIVLFDDTVRSNIAFGRTGAGQ
                     ADVEQAAKSAYAHDFILRLPEGYDTIIGERGLKLSGGERQRLAIARAILRDPPLLILD
                     EATSALDTESERIVQLALANLMKNRTTLVIAHRLSTIQNADRIIVLDRGAIVEMGSHD
                     ELLRRGGVYRRLHALQFQDVTSV"
     gene            719..1357
                     /locus_tag="VN039_06670"
     CDS             719..1357
                     /locus_tag="VN039_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWV45691.1"
                     /translation="MSDLIKSCARWIKCSLLPPLGAAAIRSVARTMHCETGGHEAVDG
                     LYREGRNIILAFWHAQQLMIPIGYRGTGSHVLISQHGDGEIIARIIARFGHDAVRGSS
                     TRGGAGALRAMIKLGRSGRDVVVTPDGPKGPCQVAKLGVIHLAKATGLPIVPLAFACS
                     KKNSLRAGIVIWSRTRFPEGYFSTGIRFGSRAKPMKPRSRRLAWSLRRSSID"
     gene            1470..2846
                     /locus_tag="VN039_06675"
     CDS             1470..2846
                     /locus_tag="VN039_06675"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HWV45692.1"
                     /translation="MWKLLYNIGFLLATPVIVGVLLAKPRCRPGLAQRLQLEECLSGL
                     SGRRDRRDRPNRPDRPDQPVIWIHAVSLGEVVAAAPLVKALHERHPEFRYIVTTVTET
                     GREAVEQRLGGIAEHRYAPLDFPWAVARMVRHLRPMLYVFVETEIWPNLLWTLREQGV
                     PSVLVNGRLSSRSFRRQNVPMIRSFYRSVLRTVTLCLMQSERDGDRLVTLGADPTRVH
                     VTGNIKFDQPLPDVRSDESLRRSFGIDEHEQLILAGSTHAGEEELLVSAYRHIVKTYP
                     ATVLMLAPRHIERAERVEATLREAGLVVQRRSRIREKQAGPRVVILDSRGELSRAYRE
                     AVVAFVGGTLVPVGGHNLLEPAVWGTPVMFGPYTDHCAEVATLLSDSGGGRRVTGEED
                     LVASLDEWLGRPETRYRVGQAARQVVLDNQGALTRSLELIETCLRAAPSYSSSCVTTG
                     PGPLMVKP"
     gene            2848..>3240
                     /gene="lpxK"
                     /locus_tag="VN039_06680"
     CDS             2848..>3240
                     /gene="lpxK"
                     /locus_tag="VN039_06680"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWV45693.1"
                     /translation="MPFLHPGQPVRKGLYWVAGLYGLIIRFRLWCYRKGWLTTTRLPC
                     RVVSVGNLTVGGTGKTPIVILLTEWLSAKGKKVAILSRGYKRMSKEPRLLVSDGSRIL
                     VGPVEAGDEPFLMAQRCPQAIVAVGADRV"
BASE COUNT          644 a          854 c         1011 g          731 t
ORIGIN      
        1 cagggcgtct ccctacggta tgagaatcag atcgctacgg ctctgaccga tgtggatctc
       61 acgatcaagc ccggcgaagt gattgcgctc gtcgggaaca gcggaagcgg aaagaccaca
      121 ttggtcggct tgctgccgcg tttctatgaa ccgacggtgg gcgtcattta catagacggc
      181 atccccttga cctcgtacga gttgaaatcg ctacgggccc atatcggaat cgtctcgcaa
      241 gagatcgtct tgttcgatga taccgtccga agcaacatcg cgttcggacg gaccggtgcc
      301 ggccaggccg acgtcgagca ggcagcgaaa tcggcctatg cccatgactt tattctccgc
      361 ctgccggaag gatacgatac gatcatcggg gaacgcggcc tcaagctatc gggcggcgaa
      421 cggcaacgcc tggcgattgc ccgagccatt cttcgtgatc ctccgcttct gatcctcgat
      481 gaagcgacat cggcactcga caccgagtct gaacggatcg tacagttggc cttggccaat
      541 ttgatgaaga atcgaacgac cttggtgatt gcacatcggc tctcgaccat tcaaaacgcg
      601 gatcgaatca tcgtcttgga tcgaggagcc attgttgaaa tgggctctca tgacgaactg
      661 cttcggcggg gaggcgtcta tcgccgtctg catgccctgc aatttcagga tgtgaccagt
      721 gtctgatctc atcaagagct gcgctcgttg gatcaaatgc tcactcttgc ctccgctcgg
      781 agccgccgcc attcgcagcg tcgcgcgcac gatgcactgt gaaaccggag gccacgaggc
      841 agtcgatggg ttgtatcggg aaggacgcaa catcattctc gcgttttggc atgcccagca
      901 gttgatgatt ccgatcggtt accgaggaac cggttcccat gtgctgatca gccagcatgg
      961 cgatggggaa atcatcgcac ggatcatcgc gcggttcggc cacgacgcgg tacgtggatc
     1021 gagtacgcgt ggaggtgccg gtgcgcttcg ggccatgatc aagctgggac ggtcgggtcg
     1081 ggatgtcgtg gtgacacccg acggccccaa ggggccgtgt caggtcgcaa agcttggcgt
     1141 cattcattta gccaaggcga caggtcttcc cattgtgccg ctggcttttg cctgttcaaa
     1201 aaaaaactct ttgcgagctg ggatcgttat atggtcccgt acccgttttc cagagggcta
     1261 tttctctacg ggaatccgct ttgggtctcg cgcgaagccg atgaagcctc gctcgagacg
     1321 actcgcctgg agcttgagac ggtcctcaat cgactgacgg atcaggcgga acaaaacgtt
     1381 agtcgttaat cgtcaagcgc ggtcccggcg cgaatgcttt cggtcattct acgtttgacg
     1441 tatgacgagc ggcggtcggt ggtgtttcca tgtggaaact tctctataat atcggctttc
     1501 ttctcgctac ccctgtgatc gtaggcgttc tcctggccaa accacgttgt cgaccggggt
     1561 tggcgcagag acttcaattg gaagaatgtc tatctggtct gtccggtcga cgagatagac
     1621 gagacagacc aaatagacca gacagaccag atcaaccagt catctggatt catgcggtgt
     1681 cgctcggtga agtggtggcg gcagcgccgc tcgtgaaggc gctgcacgag cgtcatccgg
     1741 agtttcgcta catcgtgacg acggtaaccg agaccggacg tgaggcggtc gaacaacgct
     1801 tagggggcat cgccgagcat cgatacgctc cgcttgattt tccctgggcc gtggcccgta
     1861 tggttcggca tctgcgtcca atgctctatg tgttcgtgga gaccgaaata tggccgaact
     1921 tactatggac gttacgagag cagggtgtgc cctccgtgct ggtgaatggg cggttgtctt
     1981 cccggtcgtt tcgacgtcag aacgtgccca tgattcggtc attctatcgc tccgtgctcc
     2041 ggacagtgac gctctgtctg atgcaatccg agagggatgg agatcgtttg gttacgttgg
     2101 gggccgatcc gacacgcgtt cacgtgaccg gcaatatcaa gtttgatcag ccgctgccgg
     2161 atgttcgatc ggatgagtct ctccgccgaa gcttcgggat cgatgagcac gaacagttga
     2221 ttctcgccgg cagtacgcat gccggcgaag aggaactgtt ggtctcggcc tatcgccaca
     2281 tcgtgaagac ctatcccgca acggtgttga tgttggcgcc gcgccatatc gagcgcgcgg
     2341 aacgagtcga agcgacgctt cgagaagcag gacttgtcgt ccagcggagg agtcggattc
     2401 gagagaagca agccggccca cgcgtagtca tattggatag caggggggag ctgtctcgtg
     2461 cgtatcgaga agccgtcgtg gcattcgttg ggggaacgtt ggttcccgtg ggtgggcaca
     2521 atttgttgga accggctgtg tggggcacac ctgtcatgtt tgggccctat acggaccatt
     2581 gtgctgaggt cgcgacgctc ttatccgatt ccggaggagg ccgccgtgtg acgggagagg
     2641 aggatctggt tgcctccctt gacgagtggt tggggcggcc tgaaactcga tatcgggtgg
     2701 gacaggctgc aagacaggtg gtgttagaca atcaaggcgc gctcacacgg agtctggagc
     2761 tcattgaaac ctgtctgcgt gcggctcctt cgtattctag ttcatgtgta acaacggggc
     2821 cgggacctct tatggtcaaa ccctaacatg ccattcttgc atcctggaca accggtccgt
     2881 aaagggcttt actgggttgc cgggttgtac ggtctcatta tccgatttcg gctgtggtgc
     2941 tatcggaaag gttggcttac gaccacacgg ctgccctgtc gagtagtgag cgtgggtaat
     3001 cttacggtcg gggggaccgg aaaaactccc atcgtcatcc tcctgacaga atggctgtcg
     3061 gctaaaggga agaaggtggc gatcttgagc cggggataca aacggatgag caaggaacct
     3121 cgccttctcg tgtccgatgg gtcgcggatt ttggttgggc cggtagaagc gggggacgag
     3181 ccgtttctca tggcacaacg ttgtccacag gcgattgtcg cggtgggagc cgatcgggtt
//
LOCUS       DATMTI010000409         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_779365, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000409 DATMTI010000000
VERSION     DATMTI010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779365"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VN039_06685"
     CDS             <1..305
                     /locus_tag="VN039_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:1990904 - ribonucleoprotein complex
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWV45694.1"
                     /translation="ITPRATEKAYAQSQENVYVFNVPMTANKQQITAAVEAQYDVTVV
                     NVKTLVQSGKAVRFSRGKNRYPGTTTRKDTKKAYVRLADGNSIKVFDVPAEAEEKK"
     gene            305..1144
                     /gene="rplB"
                     /locus_tag="VN039_06690"
     CDS             305..1144
                     /gene="rplB"
                     /locus_tag="VN039_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWV45695.1"
                     /translation="MPIKAYNPTTPARRGMTTQDLSEITTRKPLKSLIKSKKQNAGRN
                     NTGRITVRHRGGGVKRHYRLMNHNLADGLKLTIEEIEYDPNRSARIARVKDQHGLYHY
                     ILADTSMTKGKVIQSGVDAPIESSNRMPLANIPVGTQIYAIEIHSGKGAQMVRSAGAK
                     AQLMAKEGDYAQIRMPSGEVRKFRLECTAAIGVVGNVQHQNVKIGSAGRNRRKGIRPG
                     VRGVVMNAADHPHGGGDGGRHGSGKAPRTPWGQLTLGYRTRRRKDTNKLIVRSRHNAK
                     RKR"
     gene            1148..1408
                     /gene="rpsS"
                     /locus_tag="VN039_06695"
     CDS             1148..1408
                     /gene="rpsS"
                     /locus_tag="VN039_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWV45696.1"
                     /translation="MSRSLKKGPYVDAKLAKKVAALDTNDRTIIKTWARASTITPDMV
                     GRTIAVHNGRMHVPVLVSENMVGHKLGEFSPTRKFRKHGGKK"
BASE COUNT          416 a          388 c          412 g          315 t
ORIGIN      
        1 tcatcacacc ccgagcgact gagaaggcat atgcgcaatc tcaggaaaac gtctatgtct
       61 tcaacgtgcc gatgactgcc aacaagcagc agatcaccgc agccgtagaa gcacaatacg
      121 atgtaacagt tgttaacgta aaaacacttg tccagagtgg taaggccgta cggttctctc
      181 gtggaaagaa ccgctaccct ggcacgacca cccgaaaaga tactaagaag gcgtatgtac
      241 gtctggctga cggcaatagc atcaaagtat tcgacgtccc agccgaagca gaggagaaga
      301 agtaatgccg attaaagcat ataatccaac tactccagcc cgacgtggta tgacgactca
      361 ggatttgagc gagattacga cacgtaagcc actcaagagc ttgatcaaga gtaagaagca
      421 gaatgcggga cggaacaata ccggacgcat cacggttcgt catcgtggtg gtggtgtcaa
      481 gcggcactac cgtctgatga accacaattt ggcagacggt ttgaaattga cgattgaaga
      541 gattgaatac gaccctaacc gctcagcccg tatcgcacgc gtgaaggatc agcacggtct
      601 gtatcattac attctggcag ataccagcat gaccaagggt aaggtcatcc aaagcggcgt
      661 tgatgctccg atcgagagca gtaaccgtat gccgctcgct aatatccccg tcggtacgca
      721 aatctacgct atcgagatcc attctggcaa gggtgctcag atggttcgtt cagctggcgc
      781 caaggcgcaa ctgatggcca aagaaggtga ctacgcacag atccgtatgc catcgggtga
      841 agtccgtaaa ttccgtctgg agtgtacggc agccatcggt gtcgtcggta acgtccagca
      901 tcagaacgtc aaaatcggtt ctgccggccg taatcgccgc aaaggtatcc gacctggcgt
      961 ccgcggtgtc gttatgaacg ctgccgatca cccgcatggt ggtggtgacg gtggtcgtca
     1021 cggtagtggt aaagcaccac gaaccccatg gggtcaacta acactcggtt atcgtactcg
     1081 tcgtcgtaaa gacacgaaca agttgatcgt acgtagccgc cataatgcga agaggaagag
     1141 gtaagatatg agtcgatcac tcaaaaaggg tccttatgtt gatgccaagc ttgctaagaa
     1201 agtggcggcc ttggacacca acgaccgaac tatcatcaag acatgggcgc gagccagcac
     1261 gattactcca gatatggttg gtcgtacgat cgcagtccat aacggccgca tgcacgttcc
     1321 cgtgctggtc tcggagaata tggtcggaca caagctcggc gagtttagtc caacacgaaa
     1381 gttccgcaaa cacggaggta agaagtaatg gctactgagt ttaccgtccg ttcatatgca
     1441 aagggcgttg atcagacgcc acgtaaggtc ggtatcgttg caactcttgt tcgtaaccgt
     1501 accgtcgccg atgcgctggt tatcctggag c
//
LOCUS       DATMTI010000410         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_439846, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000410 DATMTI010000000
VERSION     DATMTI010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439846"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VN039_06700"
     CDS             <1..688
                     /locus_tag="VN039_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWV45697.1"
                     /translation="IIRDGFKVALVGEPNAGKSTLLNALSGRDVAIVTDIAGTTRDVL
                     SVDINLEGYLVRIFDTAGIRETQDVVELEGVRRAVLTAETADLILLLQDNDSTPKQSL
                     ELFKNRSYLRVRTKAALHPTTLDDQYDLSISAKEGIGLTELRSVLKQEIEKRVGAGQT
                     LVPARARHKKRLEETLNYVSDALDSATPDLAIRSEYLRLAATSLGRITGRVDVEDLLG
                     VIFSEFCIGK"
     gene            752..>1615
                     /locus_tag="VN039_06705"
     CDS             752..>1615
                     /locus_tag="VN039_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV45698.1"
                     /translation="MHQSFDVIVIGGGHAGSEAAAAAARHGAKTALVTHKRETIGVMS
                     CNPAIGGLGKGHLVREIDALDGLMGRVADAAGIQFRMLNRKKGPAVRGPRTQADRRLY
                     REAMQREIDAIGNLTVIEGDAFDIEMADNRVAAVIMKNGSRIPCGAVVLTSGTFLRGL
                     IHIGSEKIPAGRVGEMPSLGLSETLSRLGLVMGRLKTGTPARLDGRTIDWNAVDRQAA
                     DEDSVPFSFMTDHIVNPQIECGVTRTTPAGHKIIQENIHLSAMYSGQIEGVGPRYCPS
                     IEDKITRFGERD"
BASE COUNT          373 a          409 c          468 g          365 t
ORIGIN      
        1 aatcattcgt gacggtttca aggttgcgct cgttggcgaa ccgaacgctg gcaaatccac
       61 cctcttgaat gccctcagcg gccgagacgt tgcgatcgtc accgacattg ccggcacgac
      121 gagagatgtc ttgagtgtcg acatcaatct ggaaggttat ctggtccgta tcttcgatac
      181 ggcaggaata cgggaaacac aggatgtcgt ggaactggaa ggcgttcggc gtgcggttct
      241 gacggcggaa acggcggatc ttattctact gctgcaagat aatgattcga ccccgaaaca
      301 atctctcgaa ttattcaaaa atcgaagcta tctccgggtt agaacaaaag ctgcacttca
      361 tcccactaca ttggacgatc aatatgattt gtctatctca gccaaagagg gaataggcct
      421 gacagaattg cgttcggtac tcaagcagga gatagaaaaa cgcgtcggcg ccggccagac
      481 tttggttccc gcccgtgccc gtcataaaaa acgacttgaa gagacgctga actatgttag
      541 tgatgcgctc gattccgcga cccctgacct tgctataagg tcggaatatt taaggctcgc
      601 cgcgacatcg ctcggacgga tcactggccg tgttgatgtg gaagatttgc tgggcgtgat
      661 cttctcggaa ttctgcatag gaaaatgatt cacgtgaaac atcggcggct gagaccgtga
      721 tgttgatgct gacggaacag ggcgcaagga tatgcaccag tcattcgatg tcattgtaat
      781 tggtggtggt cacgcgggca gcgaagccgc agccgcagct gcaaggcatg gcgcaaaaac
      841 cgcccttgtg acccacaagc gcgagacgat tggcgtcatg tcctgtaacc ccgctattgg
      901 cggcctcggc aaggggcatc tcgtccgtga gatcgatgcg ctggatggtt tgatgggccg
      961 cgttgccgac gcggcaggta ttcagttccg catgctcaat cgcaagaaag gtcccgctgt
     1021 tcgcgggccg cgtacacagg ccgatcgacg gctttatcgc gaggcgatgc agcgtgagat
     1081 cgatgcgatt ggaaatctga ccgttattga gggcgatgct ttcgatattg aaatggccga
     1141 taatcgggtc gccgcggtca tcatgaaaaa cggtagccgg attccttgcg gcgccgtcgt
     1201 gctgacgagc ggcaccttcc tgcgtggtct cattcatatc ggttccgaaa agatcccggc
     1261 aggacgcgtt ggagaaatgc cgtcccttgg tctctcggaa acactgtcgc gactgggtct
     1321 tgttatggga cgcctgaaaa caggaacgcc ggcccgactg gacgggcgta cgattgactg
     1381 gaatgcggtg gatcgtcagg cggctgatga ggattctgtc ccgttttctt tcatgacgga
     1441 tcatattgtt aatccccaga tcgaatgtgg cgtcactcgc accacgcctg caggtcacaa
     1501 gatcattcaa gagaatattc atctatcggc gatgtattcc ggacagatcg aaggggttgg
     1561 tccacgttat tgcccatcaa tcgaggacaa aatcacccgt ttcggcgagc gggac
//
LOCUS       DATMTI010000411         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_694216, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000411 DATMTI010000000
VERSION     DATMTI010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694216"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2204
                     /locus_tag="VN039_06710"
     CDS             <1..2204
                     /locus_tag="VN039_06710"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWV45699.1"
                     /translation="LAIVLGVIAYGVIPLVDSLELKWFVRDLDMRSKLMVNTMEGPLT
                     DLLVSDSKDKILAYFTRIRQDERLYALGFCDLDHHLLYETQAYPQDVTCKDTLDLASN
                     SSTVRSFSSGPLHITSASIESNGRRLGRLLLIHDMSFIQQRSSDTKWYVFYLFAGLTA
                     VISLVTVLVAHFSRKEWVAGVRAMLKGERLLTPLTHEEHAPELQPLAKDLRSLVQALE
                     SDRRMRDESQISWTPVSLKTLLHEQLAGDQVLIVSNRQPYSHYWQDRNIVVQMPASGL
                     VSALEPVMRACSGIWVAHGSGSADREVVDGRDHVGVPPSHPMYEIRRVWLTEEEEAGY
                     YYGFANEGLWPLCHIAHVRPTFRSSDWNHYVAVNERFAQAVYEEATTDNPVVLVQDYH
                     FALLPKLIRDKLPTATIIMFWHIPWPNAESFGICPWREEILEGLLGSSILGFHTRVHC
                     NNFIDSVDRILEARIDRNSSTVSYGGKMTAVNPYPISIEWPMQWLHDQRSVPECRTRL
                     RELYGMSPDRLVGLGVERLDYTKGILERFMAVERLLELQPEWIGKFTFLQIAAPSRST
                     IEQYHHFTGQVSALAEQINKRFGRDGYEPICLRIQHHGASQVCECYRGADLCVVSSLH
                     DGMNLVAKEFVGARDDEQGVLILSQFTGAARELTEAVVINPYDIDQFAAALHLGLTMP
                     KVEQRARMQSMRGLIQEFNVYRWAGRMLIDAARMRQKERVMKQVRRPSRLN"
     gene            2215..>2988
                     /gene="otsB"
                     /locus_tag="VN039_06715"
     CDS             2215..>2988
                     /gene="otsB"
                     /locus_tag="VN039_06715"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWV45700.1"
                     /translation="MDYLLTETGRTDLEALLKVRSLYAFDFDGTLAKIVRDHHAARLA
                     RPIRLRLEALAERAPTAIISGRSVEDLRSRVGTAVPHLIGNHGSEGPHTHPEDLRQVR
                     ETSLGWLQLINGRFQDELTRRGVVVEGKSFSLSFHYRTVDRRDEARALISRIVAELSP
                     PPRIVLGKSVVNVMPPTASHKGTALLEHMRRLDCSTALYVGDDETDEDVFALRDARIL
                     TVRIGKKNGSSARYFLKRQAEIAEVLRLLVEVGDQSVHTW"
BASE COUNT          628 a          809 c          882 g          669 t
ORIGIN      
        1 cattggcgat tgtcttggga gtgatcgcct atggcgtgat cccgctcgtc gactcgttgg
       61 aattgaaatg gttcgttcgc gatctcgaca tgcggtccaa actcatggtc aatacgatgg
      121 aggggcctct tacggacctt ctggtatccg attcaaagga caagatcctg gcctatttta
      181 cgcgcattcg tcaggacgaa cgattgtacg ccctgggatt ttgtgatctc gaccaccatc
      241 ttctctatga aactcaggca tacccacagg acgtcacctg taaagataca ctcgaccttg
      301 catccaattc gtccaccgtt cggtcattca gcagcgggcc gctccatatc acgtcagcct
      361 cgatcgaatc aaacgggcgc cggctgggcc gtcttctgct catccacgac atgagtttta
      421 tccaacaacg gagcagcgat acgaaatggt acgtcttcta tctgttcgca gggctgacgg
      481 ccgtcatttc gctggtcacc gtgttggtgg cacatttcag tcggaaagaa tgggtggccg
      541 gagttcgagc catgttgaag ggagaaaggc ttctgacccc gctgacccat gaagagcatg
      601 ccccggaact ccagccgttg gcgaaagact tgcgttcgtt ggtgcaggct ctagaatctg
      661 atcgccgtat gcgcgatgag agccagattt cgtggacccc ggtcagcctc aaaacgctcc
      721 ttcacgagca gcttgccggc gatcaggtgt tgattgtgtc caatcgacag ccctatagcc
      781 actattggca ggatcggaac atcgtcgtgc agatgccggc gagcgggctt gtgtcggcgc
      841 tcgaaccggt gatgcgcgcc tgctccggta tatgggtcgc gcatgggagc ggatcggcgg
      901 accgagaagt ggtggatgga cgggaccatg tcggtgtgcc gccgtcgcat ccgatgtatg
      961 agattcgccg cgtgtggctc accgaggaag aagaggcagg ctactattac gggtttgcca
     1021 acgagggatt gtggcctctc tgtcatatcg cccatgtccg gcccacgttt cgttcatcgg
     1081 attggaatca ctatgtggcc gtcaacgagc gatttgctca ggctgtgtat gaggaggcca
     1141 cgaccgacaa tccggtcgtc ctggttcagg attatcattt tgccctgctt ccgaagctga
     1201 ttcgagataa attgccgact gcgacgatca tcatgttctg gcacattcca tggccgaacg
     1261 cggaaagttt cggcatctgt ccctggcgcg aagaaattct tgaggggctg cttggaagca
     1321 gtattctggg gtttcacacc cgcgtgcact gtaacaactt catcgacagt gtcgatcgga
     1381 ttctcgaggc gaggatcgat cgaaacagtt caacggtctc ctatgggggc aagatgacgg
     1441 cggtcaaccc ctatccgatt tccatcgagt ggcccatgca atggctccat gaccaacggt
     1501 cggttccgga atgccggacc agacttcgag agttatacgg gatgtctcct gatcgcctgg
     1561 tcggccttgg ggtggaacgc ctggattata cgaaggggat cctggaacgg ttcatggcgg
     1621 tggaacggtt gctggaactc cagccggaat ggatcgggaa gtttacattt ctgcagatcg
     1681 ccgctcccag ccgttcaacc atcgagcaat accaccattt taccggccag gtctctgcac
     1741 tagccgagca gatcaacaag cggtttggtc gggacggcta tgaaccgatc tgcctgcgga
     1801 ttcaacatca tggggcgtct caggtctgcg aatgttaccg tggagcggac ctgtgcgtgg
     1861 tcagcagtct tcatgacggc atgaatttgg tcgccaaaga gttcgtcggc gccagggacg
     1921 acgagcaggg agtattgatt ctgagtcaat tcacgggagc cgctcgtgaa ctgacggagg
     1981 cggtcgtaat caatccctac gacatcgatc aatttgcggc tgcgctccac cttggcttga
     2041 cgatgccgaa ggtggagcag cgggccagga tgcagagcat gcgaggactg atccaggaat
     2101 tcaatgtgta tcgctgggcc ggtcgtatgc tcatcgatgc agcccgcatg agacaaaagg
     2161 aacgagtcat gaagcaggtg cggcggccga gccggctgaa ttgatggacg gacaatggat
     2221 tatttattga cggaaacagg aagaaccgac ctcgaggccc tcctgaaggt ccgttctctg
     2281 tatgcgtttg atttcgacgg gactttagcc aaaatcgtcc gagatcatca tgccgccagg
     2341 ttggcgcgtc ccatccgcct ccggctcgag gcactcgcgg agcgcgctcc caccgccatc
     2401 atctcgggac gctccgtgga ggacctgcgt tcgcgtgtcg ggactgccgt gccccacctg
     2461 atcgggaacc atggttcaga aggccctcat acgcacccag aggatctgcg gcaagtccga
     2521 gagaccagtt tgggatggct gcaattgatc aacggacgat ttcaggacga gctgactcga
     2581 cgcggcgtag tcgtcgaagg taagtccttt tcactctcat tccactatcg aaccgtcgat
     2641 cggcgagacg aggcgcgggc gctgatctct cgaatcgttg ccgaattgtc tcctcctcct
     2701 cgcattgtcc ttgggaaatc cgtcgtcaat gtgatgccgc cgacggcgtc gcacaagggt
     2761 acggcattgc tggagcacat gcgccgactt gactgcagca cggctctcta cgtgggagac
     2821 gacgaaaccg atgaagatgt ctttgcgcta cgagatgccc gcattctcac tgtgaggatc
     2881 ggcaagaaga acggttcctc tgcccggtat ttcctaaaaa gacaggcgga aatcgccgag
     2941 gtgcttcgac tgcttgtcga ggtcggcgac caaagtgttc atacctgg
//
LOCUS       DATMTI010000412         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_185989, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000412 DATMTI010000000
VERSION     DATMTI010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185989"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="tgt"
                     /locus_tag="VN039_06720"
     CDS             complement(<1..939)
                     /gene="tgt"
                     /locus_tag="VN039_06720"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWV45701.1"
                     /translation="MMQYHVQHFDRHSKGRIGLLTTGHAAVNTPAFMPVGSLGPVKGL
                     EPEDLQSLGFRLILNNAYHLYLRPGHKLVADMGGLHAFTGWPGAILTDSGGFQIFSLA
                     KLCEITDAGVTFQSHIDGSTHFITPEKTIEIEEALGADIVMVLDQCAALPAGREIIEE
                     SVRRTKLWAERCQASRRRTDQALFGIVQGGLETDLRVASARELVKLGFEGYAIGGLSV
                     GEDKSDMYAMLDVTVPELPEKKPRYLMGVGHPEDLLEGVARGIDLFDCVVPSRHGRTG
                     SLFTATGRVVIKQAQYAEDERPIDPACTCPVCRRYSR"
     gene            complement(962..2686)
                     /gene="argS"
                     /locus_tag="VN039_06725"
     CDS             complement(962..2686)
                     /gene="argS"
                     /locus_tag="VN039_06725"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWV45702.1"
                     /translation="MSQGLVQERVTTALLGALNDARQKGQLKTTAWPTLSLDAPKRPE
                     WGDLASTVAMSLASTEHKAPHDIAQIIIENLPQREQLFDRVEIVRPGFLNLTVKPALW
                     QEVLREIEFQGARYGRAAVGTGRRVLVEYVSANPTGPLHVGHGRGAAVGDAVVRLLNA
                     IGYDAVGEYYINDAGRQMKLLGASVYARYQELSERTVEFPEEGYHGSYITAVAQQIKE
                     QLDRVADGLTPADLEARCRTLAYQILLGLIRDDLQSFGIEIQSWFSEASLLESKAIER
                     ALAELKSRDLLFQQEGAWWFRASIFGDEKDRVVKKQDAEYTYLASDIAYHHDKLRRGY
                     DLLIDVFGADHHGYIPRMQAVMQAYGHPKDRLHVVLVQLVKLLRDGIEVKMSKRTGEF
                     ITMREVIDEVGADAAKFYFLMRDSKTHLEFDLELAKQRSADNPVYYVQYAHARICSLW
                     RVASERGMTRPPALETDLTVLTDPDELGLIKKLSAYPEIIQASALAFEPHRVTYYLQQ
                     LAALLHTFYNKHRVLPPAADQEHDESASAETLTPKRTAARLVLMGAVQQVLRNGLEVL
                     GISAPEQM"
BASE COUNT          660 a          871 c          769 g          661 t
ORIGIN      
        1 ccgtgagtat cgacggcaga ccggacacgt gcaagcagga tcgatcggtc gttcatcctc
       61 agcatactga gcttgcttga taacgacccg ccctgttgcc gtaaacagcg aacctgttct
      121 gccatggcgc gagggaacga cgcaatcgaa gagatcgatg ccgcgggcca ccccttcaag
      181 cagatcttcc ggatgtccga cccccatgag ataccgaggc tttttctccg gcaactctgg
      241 aaccgtcacg tcgagcatcg catacatgtc ggacttatcc tccccgaccg acagcccgcc
      301 gatcgcatag ccctcgaacc ccagtttcac caattccctc gccgaggcta cgcgcaagtc
      361 cgtttccaga ccgccctgta cgatgccaaa caaggcctga tccgttcttc gcctgctggc
      421 ctgacaacgc tcggcccaaa gcttggttcg acgcacactt tcctcgatga tttctcgacc
      481 ggcaggcaac gccgcgcatt gatccagcac catgacgata tcggctccca atgcttcttc
      541 gatttctatc gtcttttcgg gcgtgatgaa gtgcgtcgac ccatcgatgt gcgattgaaa
      601 tgtgacgcct gcatcggtga tttcgcacaa cttcgctaaa ctgaagatct gaaacccacc
      661 gctgtcggtg aggatcgctc ccggccatcc ggtgaaggca tgaagcccac ccatatcggc
      721 aacgagcttg tgaccagggc gcaaatacaa atgataggca ttattgagga tgagtcgaaa
      781 ccccagactc tgcaggtcct ccggttcgag acccttgact ggtcccagcg agcctaccgg
      841 catgaaagcc ggtgtgttga ccgctgcatg accggtcgtg agcaggccga tgcggccttt
      901 ggagtgccgg tcgaaatgtt ggacgtggta ttgcatcatc tattatggga ggtctgaccc
      961 gctacatttg ctcaggcgct gaaatgccga gcacctcaag tccatttcta agaacctgct
     1021 ggacagcccc catcaagacc agacgcgccg cagttcgttt cggcgtgagg gtttcggcgg
     1081 acgccgactc gtcatgctcc tgatcggccg caggaggcaa cacacggtgt ttgttataaa
     1141 acgtatggag aagcgccgcc aattgctgga gataatacgt cactcgatgc ggctcaaagg
     1201 ccagcgcgct ggcttggata atttcagggt aggcggaaag ttttttgatc agccccaatt
     1261 catcggggtc ggtcaaaaca gtcaggtccg tctccaatgc gggcggtcgg gtcattcctc
     1321 tttcggatgc cacacgccag aggctgcaga tcctcgcatg ggcatattga acgtagtaca
     1381 ccggattgtc ggcggaccgt tgcttcgcca actccagatc gaattcgaga tgagttttgg
     1441 agtcccgcat taagaaatag aatttcgcag cgtcggctcc gacctcatcg atgacttccc
     1501 gcatcgtaat gaattccccg gtccgcttag acatcttcac ttcgattcca tcccgtagca
     1561 gcttcaccaa ctgaaccagc acgacatgga gacgatcttt tggatgtcca taggcctgca
     1621 tgacggcttg catgcgcggg atatacccat ggtggtcggc gccaaagaca tcaatcagca
     1681 gatcgtagcc acgcctcagc ttgtcatggt gataggcgat atcggacgct aagtatgtat
     1741 actcagcgtc ctgcttcttg acgacacggt ctttttcgtc gccgaatatg gatgctcgaa
     1801 accaccatgc cccttcctgc tggaacagga ggtcgcggga ttttaattca gccagagctc
     1861 gttcgatggc tttggactct agaagcgaag cttcgctgaa ccaggattgg atttcgatac
     1921 caaaagactg aaggtcatca cgaatgagtc ctaacaggat ctgataagca agagttcggc
     1981 agcgagcctc aagatcggcg ggagtcagtc catcagcgac gcggtcgagt tgctccttga
     2041 tctgctgtgc cacagcggtg atgtacgagc catgataacc ttcctcagga aactcgacgg
     2101 tccgctccga caactcttga taccgagcat agacagatgc gcccaacaat ttcatctgtc
     2161 gtcccgcgtc gttgatatag tactcgccca cggcatcgta tccgatcgca ttcagtagcc
     2221 ggacaactgc gtccccgacc gccgcccccc gtccatggcc gacatgcaat ggaccggtcg
     2281 gattggcgct cacatactca accagcactc gacgaccggt tccgacagct gcccggccat
     2341 agcgagcccc ttggaactcg atttcacgga gaacctcctg ccagagggcc ggtttcaccg
     2401 taagattcaa gaaaccggga cgaacgattt ccacacgatc gaacagctgc tctctctgag
     2461 gaagattttc tatgatgatt tgggcgatat cgtggggtgc cttgtgttca gtggaggcca
     2521 aagacatcgc caccgtcgaa gcgagatcgc cccactccgg ccgtttgggc gcgtcaaggc
     2581 taagtgtggg ccaagccgtc gtcttcaact gccctttttg cctcgcgtca ttgagggcgc
     2641 caagcagggc cgttgttacc cgctcttgca cgagtccttg agacacaaca attctctcta
     2701 agcccttacc aaagaaagga aattaacgcg ccactttatc acatgtgata ggtagagaca
     2761 aggcggttca tgtggaatta cgagggtttg tgctgctcct tccccctctc aatgctgtca
     2821 gaatctcttc tatcggcact tgaagacaag gtttctccgc acactttctc actgtttccc
     2881 acgactcaca gcgacaggat gacccacgca ggatcgtcac atgactacca tatggggccc
     2941 atcgctgtgg gtctgtagga c
//
LOCUS       DATMTI010000413         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_737088, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000413 DATMTI010000000
VERSION     DATMTI010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737088"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1571
                     /gene="sthA"
                     /locus_tag="VN039_06730"
     CDS             159..1571
                     /gene="sthA"
                     /locus_tag="VN039_06730"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HWV45703.1"
                     /translation="MRSYDLVVVGSGPAGQKAAVQAAKLSKRVAIIEKAPQLGGTSLN
                     TGTLPSKTLKDTIEYVHGLGRRGLCQLGAELTKQLTLPDLMTRKNQVIETEVAVITNQ
                     LRRNGIEIIQGTAGFVDPHTLSVVRSDGHVDHVHASIIILATGSRPRRPAEIPFDDLI
                     ICDSDSFLRTTKNPAGIIVIGGGVIGTEYASMLAAFGIRVTLIDRRTQLLRFLDQEIA
                     QALDSQIQQNGVMVRLGQDQLDISMNEAGYPVVRFQDGETVTADMVLYTMGRVGNTEA
                     LNLPVIGLTTDQQGQLVVNAQYQTAVPHIYATGDVIGFPALAATAMEQGRLAACHAFH
                     VSEAHDIKVIPYGIYSIPEVSMVGKTEEELTVADVPHATGRAFFREMARGHISGDLHG
                     LLKVIFHRETHALLGVHIIGPSATELIHIGQSVLTYGGTVEYFVHNVFNYPTMAECYR
                     TAALDGLNRLHHHPRHVEPT"
     gene            1824..2306
                     /locus_tag="VN039_06735"
     CDS             1824..2306
                     /locus_tag="VN039_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWV45704.1"
                     /translation="MPIPWTLKSHLDREHVHYDVLPHSQTFRALAVARTLHLPIQEMA
                     KVVIVKVKEQFVTTVLPATAKVDVPRLRKLFGTHHVRLATEEEIAQLFPDCELGAMPP
                     LGTLYGLPVYVDRSLTGDEAIVFEGGTHSEAIRMRYWDFAALVFPVVAEFHRPPVATC
                     "
     gene            2473..3486
                     /locus_tag="VN039_06740"
     CDS             2473..3486
                     /locus_tag="VN039_06740"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HWV45705.1"
                     /translation="MVKRPHLIAVFLIASLIGLPTLSFAKARGGGGFSSGARGGNSSM
                     GIGSRGSRTYQDNGAKPIEQSTTQKPSATPPPNASGAPPMQPAPATTSSWWQRNPLLA
                     GIAGGLAGTWIGHMLFGATESSAKTTDAEPGAASNSASNSFGLVILLMLIGAGALYYF
                     KKVRQNPVPVFTGLSRGNTARGSLLDISSNSTDRPTTDTAYTVTTEDKTAFQQLLTDI
                     QSAWSAQDVAALRPFVTPEMLSYFSTALAEDSSRGVQNHVEGVELLKGDVREAWSEDD
                     QDYATVDLRWNARDYTVSTTIPRGEPGYLIEGSEETPTESYEVWTFMRVRDGRWLLSA
                     IQQ"
BASE COUNT          809 a         1096 c          986 g          753 t
ORIGIN      
        1 aattacaact catccgtcgc ctattcaagc gactatgccg gtgtgacagc ctgtctgtga
       61 acttgcggat tcagacttca tctatttcct tggtaggcgg gactcaccac gatatggtag
      121 gcactacgta tcgacgaaac agatcaccga gatccgctat gcgctcctat gacctggtag
      181 tggtcggcag tgggccagcc ggccagaaag cggccgtcca agcggcgaaa ctatccaagc
      241 gcgtggctat catcgagaag gcgccacagc tgggcggaac gtctctcaat accggaaccc
      301 tccccagcaa aaccctgaaa gacacgatcg agtacgttca tggattggga cggcgaggat
      361 tgtgccaact gggcgccgaa ctcaccaagc agctgacgct cccggacctg atgacgcgca
      421 aaaaccaggt catcgagacc gaagtcgccg tcatcaccaa tcagctgcgg cgtaacggca
      481 tcgagatcat ccagggcacg gccggctttg tcgacccgca cacgttgagt gtggtgagat
      541 cagatgggca cgtcgatcat gtccatgcct cgattatcat cttagccaca gggtcacgcc
      601 cacgccgtcc agcggagatc cccttcgatg acctgatcat atgcgattcc gactcgtttc
      661 tccgtacgac caagaacccc gccggcatca tcgtcatcgg tgggggtgtc atcggtacag
      721 aatatgcctc gatgttggcc gccttcggga tcagagtcac cctcatcgac cggcggaccc
      781 agttattgcg gttcttggac caggaaatcg cccaagctct cgattcccaa atacaacaga
      841 atggggtcat ggtgcggctc gggcaggacc aactcgacat ctccatgaat gaagccggat
      901 accccgtggt ccggttccaa gacggcgaaa cagtgacggc cgacatggtg ctctatacga
      961 tggggcgagt cggcaacact gaggcgttga atctccccgt catcggcctg actaccgatc
     1021 agcaggggca actcgtggtc aacgcccagt accaaacagc cgttcctcat atctacgcga
     1081 caggcgatgt catcggcttt cccgcactcg ccgcaacggc gatggaacaa ggccgcctcg
     1141 ccgcctgtca tgcgtttcac gtctccgagg cgcacgacat caaggtgatt ccctacggta
     1201 tctacagcat tcccgaggtc tcgatggtgg gaaaaaccga agaggaactc accgttgccg
     1261 acgtcccgca cgccaccggg agggcctttt tccgagaaat ggcgcgtggc cacatcagcg
     1321 gcgacctcca tggcctccta aaggtcattt ttcatcgtga gacgcacgcg ctgctgggtg
     1381 tccatattat cggaccgagc gccaccgaat tgattcacat cggccaatcg gtcttgacct
     1441 atggaggaac cgtggaatac ttcgtccaca acgtgttcaa ctaccccacg atggccgaat
     1501 gctaccgcac cgcagcactc gacggattga atcggctgca ccaccacccc cgtcacgttg
     1561 agccaaccta ggaccgcttc acacagccgt aggctttgcc catccggcgg aaagtttatg
     1621 gcatcgacac aatacggctt gcacaaacgc acaaaaaagc atataagcaa ccgatactga
     1681 cggtcatcat gacctacgtg gagtcgtagt catgagcatc atctcgacca tcgtcttctc
     1741 atccccattg cagttgcagt cccctgtctt tatcacccac caacgatttc cgtttggggt
     1801 cggccgccac aggaggtatc atcatgccca ttccatggac gcttaaaagt catctcgatc
     1861 gtgaacatgt tcattacgat gtcttgccgc actcacagac gttccgtgct cttgccgtcg
     1921 cacggacact ccacctcccg atacaggaga tggcgaaggt cgtcatcgtg aaagtgaagg
     1981 agcaattcgt tacgacagtg ctgccggcaa cggcgaaagt ggacgtcccg cgcctgcgaa
     2041 agctcttcgg aacacaccac gtgcggctgg cgacggagga agagattgcg cagctctttc
     2101 cggattgcga attgggggcc atgccgcccc tcgggacact ctatggactt ccggtgtatg
     2161 tcgatcggtc gctcactggt gatgaagcga ttgtctttga agggggtacc cactcagagg
     2221 caatccgcat gcggtactgg gattttgccg cgttggtgtt tcccgtcgtg gccgagttcc
     2281 accgtccgcc cgtggcgacc tgttgacctg gcttgggtct ctgcccgaca catcttgtaa
     2341 aaaactgatg cagatcagtc agtgctcgct gtgttcatcc ccgcagtgaa tggtaggttt
     2401 tcttcgcaca atcctgttag gatgaagcct gtgcctatca aaagtcccat ccttcttgtg
     2461 aaaggaaacg tcatggtgaa acgtccacat ctcatcgcgg tctttctcat tgcttcgctc
     2521 atcggcctac cgaccctctc gtttgcgaag gcgcgagggg gaggagggtt ttcaagcggg
     2581 gctcgcggcg gaaactcatc gatggggatc gggagccggg gatcccgtac ctaccaagac
     2641 aacggcgcca agccgattga acagtccacc acccaaaagc cgtcggcgac gccaccaccg
     2701 aatgcatcgg gcgctccgcc aatgcaaccg gctccagcca cgacgtcatc ctggtggcaa
     2761 cgcaatcccc tgctcgccgg aatcgccggt ggtctcgccg gaacctggat cggtcatatg
     2821 ctctttggcg cgacggagag cagcgctaag actacggacg cggagccggg tgcagcgtcc
     2881 aactccgcat ccaactcctt tgggctggtc atcctgttga tgttgatagg agccggtgcg
     2941 ctctattatt tcaaaaaagt ccgtcagaac cccgttccgg tgttcaccgg actctcacgt
     3001 ggcaacaccg cgagggggag tctgctcgat atctcgtcca attccaccga caggccgaca
     3061 actgatactg cctatacagt gaccaccgaa gataagacag cctttcagca actgctgacc
     3121 gacattcaat ccgcctggag cgcccaagat gtggcggcct tgagaccatt tgtgacgccg
     3181 gaaatgttga gctatttcag tactgccctg gccgaggaca gcagtcgagg cgtgcaaaat
     3241 cacgtggaag gggtggagtt gctcaaggga gatgtgcggg aagcctggtc cgaagacgac
     3301 caggactatg caaccgtgga cctacgctgg aatgccaggg actacacggt ctccacgacg
     3361 atcccacgcg gagagccagg ctatctgatt gagggcagtg aagaaacgcc gaccgaatct
     3421 tacgaagtct ggaccttcat gcgcgtgcgg gatggacgtt ggctcctatc ggccatccaa
     3481 caatagtcca cataagaggg cagctgacct tgtgagtcag ccgccctctt ggctcatcgg
     3541 aggaggatga gcctccccac caataccgtt tctcagccga tctcttcttt gtgacaccca
     3601 ttcttcattt gaactagtgg gagtcccagt tctattccgt tcat
//
LOCUS       DATMTI010000414         4897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_609796, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000414 DATMTI010000000
VERSION     DATMTI010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609796"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VN039_06745"
     CDS             complement(<1..296)
                     /locus_tag="VN039_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HWV45706.1"
                     /translation="MMSAGPMKQRIFGLENEYGLIFSPNGRIYLPMEKVLGYIFEGLI
                     PNSWPSNAFLVNGARFYQDTGCHPEYSTPECDNILDLVVHDKAGERLLEACLPAA"
     gene            complement(369..1055)
                     /gene="prcA"
                     /locus_tag="VN039_06750"
     CDS             complement(369..1055)
                     /gene="prcA"
                     /locus_tag="VN039_06750"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HWV45707.1"
                     /translation="MPMPYYVSPEQMMQDKAEYAKKGIAKGRSIIAMEYIDGILLTAD
                     NPSASLHKVSEIYDNIAFAGAGKYSEFENLRKAGIRHADLRGFMYSREDVTGRSLANG
                     YSQSLGTVFSQEMKPLEVEILVVQAGTNHHPNEIYRISFDGSIIDEKNFAVIGGRSEA
                     VQTVLKEKSPGQVPPLDAALKLCVTALEQTANQKLQPEGLEVAVLDRTRTGRKFRRLS
                     ITDVRNILSS"
     gene            complement(1058..1897)
                     /gene="prcB"
                     /locus_tag="VN039_06755"
     CDS             complement(1058..1897)
                     /gene="prcB"
                     /locus_tag="VN039_06755"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HWV45708.1"
                     /translation="MNGGSTIAMKLPYLPDHDGSSFFDFVSTHHPELALRSRGSAAGL
                     DQMRTVGPVAVPSATTVLALKYQQGVVIAGDRRATEGFQIADRRIEKVFKIDDWSAMA
                     IAGAAGPCIEMARLFQTELEHYEKLEGMPLSCEGKANKLGQMVKANLPMVFQGLVVMP
                     LYVGYDVKRSEGRIFKYDITGGRYEESDYHAIGSGGKDARNTMREHFQRHLPEGDAIK
                     LALLALYNAADDDVGTGGPDLVRGIYPTAKVVTAQGISDVSDDTLRTVCNDVMAARRS
                     RET"
     gene            complement(1897..3369)
                     /gene="dop"
                     /locus_tag="VN039_06760"
     CDS             complement(1897..3369)
                     /gene="dop"
                     /locus_tag="VN039_06760"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HWV45709.1"
                     /translation="MRVLGTETEFGIAAKDPSGVDPVSGSFAVIGHYPRLPAPGALWD
                     YENENPLLDARGFEVDGERERPNPDYNRQLNKVLANGGRLYVDGAHPEYSTPECSNAR
                     EIVAFERVGERILAACLQEMARVRGSEQYVLYKNNSDGKGNSYGYHENYLVSRSVPFD
                     RIARVLTPFLVTRPIFSGAGKVGAENQTSPTEYQISQRADFFECLMDLNTMVKRPIIN
                     TRDEPHADAARFRRLHVITGDANMAELSTYLKVGTLDIVLDLLESGADLPQFELDEPV
                     RAFKQVSRDLDVKETVKLAGGRPTTALAVQRAYLSAAKTFYASQDHSPIIHDVLLRWE
                     DVLNQLDRDPRLLVNALDWVAKRHMIESYMERKGCGWDDPRMKLMDLQYHDVRPDKGL
                     FYTLERSRLIERIVEEEDIQRAELNPPTGTRAYFRGRCVSKFAKVLYGASWTSVLFDV
                     GNTTIKKVPLLDPYRGTQALTRELLDTADSAGALIEKLKV"
     gene            complement(3404..>4897)
                     /gene="arc"
                     /locus_tag="VN039_06765"
     CDS             complement(3404..>4897)
                     /gene="arc"
                     /locus_tag="VN039_06765"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HWV45710.1"
                     /translation="TEVEKLTAPPSAYAIFSSMNADGTGNIYVSGRKMKVSLHPSIKR
                     NELRKGQEVVLNEALNVIEVKGFDSQGEVVRLKDVLEGGRALVTLHFDEEKVAELGEP
                     LLAERLSVGDHLLYDPRSGYVIEKLPKSEAEELVLEEVPDVDYEHIGGLQKELEQVRD
                     AVELPFLHPQIFSEYKLSAPKGVLLYGPPGCGKTLIAKAVANSIAKKLGHRSGKEIRS
                     YFLHVKGPELLNKYVGESERQVREVFKKAKERAEDGHPVIVFFDEMDALFRTRGTGIS
                     SDIESTIVPQFLSEIDGVERLRNVIVIGASNRQDLIDPAVLRAGRLDVKVKVGRPDAT
                     AARDIFSKYISTDLPFAAEDLERHGGDRQALVDRLTALTVETMYAASEENKFIEVTYA
                     NGEKEILYFRDFASGALIEGIVSRAKKFAVKRVIAQEGVGLHSDDLIRAIREEFKEHE
                     DLPNTTNPDDWAKVAGKKGEKIIHIRTISGGPAEHRQIETISTGHYL"
BASE COUNT         1036 a         1464 c         1267 g         1130 t
ORIGIN      
        1 gctgcaggca aacaggcttc caacaagcgc tcgcccgctt tatcgtggac cacgaggtca
       61 aggatgttgt cgcattccgg agtcgagtat tcgggatgac agccggtgtc ttgatagaac
      121 cgcgccccgt tgaccaagaa agcgttcgac ggccagctgt tcggaatcaa tccttcaaag
      181 atatacccca ggaccttttc catggggaga tagatacggc cgttggggga aaaaatgagg
      241 ccgtattcgt tctcgaggcc gaatatccgt tgtttcatgg gaccggcaga catcatgaat
      301 gaccttcaaa cagattatac gccgcttttc ggttcttcaa caaaggaagc ctctacggag
      361 ggcagaggtc aggaagagag gatgttccgg acgtcagtga tcgataagcg gcgaaacttt
      421 cggccggtac gggttcggtc caagaccgcg acttccaacc cttccggctg cagcttttga
      481 ttggccgtct gctccagcgc cgtgacacac agcttcaatg cagcatccaa gggaggaacc
      541 tggccgggac ttttttcttt cagcacggtt tgcaccgcct cagaccgtcc gccgatcacc
      601 gcaaagttct tctcatcgat gatgctgccg tcgaacgaaa tacgatagat ttcgttcggg
      661 tgatgattgg tcccggcttg caccacgagg atttcaacct caagcggctt catttcctga
      721 ctgaacacgg tccccaggct ttgcgaatac ccgttcgcaa gcgagcggcc ggtgacatct
      781 tcccggctat acatgaatcc tctgagatcg gcgtggcgaa tccctgcctt tcggagattt
      841 tcaaactcgc tatacttacc ggcacccgca aacgcgatgt tgtcatagat ttccgacacc
      901 ttgtgcagcg aggcgcttgg attatccgcc gtcaacagaa tgccgtcgat gtactccatc
      961 gcgatgatcg agcggccttt ggcgatcccc ttcttggcat actcagcctt atcctgcatc
     1021 atctgttcag gggacacgta atagggcatc ggcatgatta cgtctccctt gaacgacgtg
     1081 cagccatcac atcgttgcac acggtacgca gtgtgtcgtc ggatacgtcg gaaatgcctt
     1141 gggcggtcac gaccttcgcc gtggggtaaa tgcctcgcac gagatccggt ccgccggtcc
     1201 cgacgtcgtc gtccgcggca ttgtagagcg ccaacaaggc taatttgatc gcatccccct
     1261 cggggagatg ccgctgaaaa tgttcccgca tggtgttgcg ggcatctttc ccaccggaac
     1321 cgattgcatg gtaatcggac tcttcgtacc gcccgccggt gatgtcgtac ttgaagatgc
     1381 ggccctcact gcgtttcacg tcatatccga cgtacaacgg catgacgacc aggccctgaa
     1441 ataccatcgg aagattggcc ttgaccatct ggcccagttt attggccttc ccttcgcagg
     1501 agagcggcat gccctccagt ttttcgtaat gctccaattc ggtctgaaat aacctggcca
     1561 tctcgatgca gggaccggcc gcccccgcga tcgccatggc cgaccagtcg tcgatcttga
     1621 agaccttttc gattcggcga tccgcaatct gaaacccttc cgtggctcgg cggtctcccg
     1681 caatcacgac accctgctga tacttgagcg cgagcacggt cgtggcggag ggcacggcga
     1741 ccggcccgac ggtgcgcatc tggtcgagac ccgccgctga tccgcggctt cgcaatgcca
     1801 actcaggatg atgtgtggag acgaagtcaa aaaaactgga gccgtcgtgg tcagggaggt
     1861 agggcagctt catcgcgatc gtcgaaccac cgttcatcaa accttgagtt tctcgatcag
     1921 ggcaccggct gaatcggccg tatcgagcag ctctcgtgtc aatgcctgcg tccctcgata
     1981 gggatccaac aggggcactt tcttgatcgt cgtattgccg acgtcgaaca gcaccgacgt
     2041 ccaactggcc ccgtacaaca ccttcgcaaa cttactgaca caacgcccac gaaaataggc
     2101 cctggtgccc gtcggcggat tgagctcagc ccgttgaata tcttcttcct cgacgatccg
     2161 ctcgatgagg cgactgcgct ccagcgtata gaacaacccc ttgtccggac ggacatcgtg
     2221 atattggagg tccatcagct tcattcgagg atcatcccat ccacaacctt tacgttccat
     2281 atacgactca atcatgtgcc gcttggcgac ccaatccaac gcgttcacca acaaccgcgg
     2341 atcgcgatcg agctgattca acacatcctc ccaacggagc aacacatcgt gaatgatagg
     2401 actgtgatcc tgcgaggcat aaaaggtctt ggcggcgctc agatacgccc gctggacggc
     2461 aagagccgtg gtgggcctcc ccccggccaa tttcacggtc tccttcacat ccaagtcccg
     2521 ggacacttgc ttgaacgcac gaaccggctc gtccaattcg aactgaggca gatcggctcc
     2581 tgactcaagc aaatccaaga caatgtccaa cgttcccacc ttcagatagg tggagagttc
     2641 cgccatgttg gcgtctcccg tgatgacatg gagcctgcga aaccttgccg catcggcatg
     2701 cggctcgtcg cgcgtgttga tgatcggccg tttgaccatc gtgttgagat ccatgagaca
     2761 ttcgaaaaag tcggcgcgct gcgagatctg atattccgtc ggactggtct gattttcagc
     2821 cccgactttt cctgctccgg agaaaatcgg tcgtgtgaca aggaacggcg tcaacacccg
     2881 agcgatcctg tcgaacggca cggatctcga gactaaatag ttttcgtggt atccatagct
     2941 gttgcccttg ccgtcggagt tgttcttgta caagacatac tgttcgcttc ccctcacccg
     3001 agccatttcc tgaaggcatg ccgcgagaat gcgctccccg actcgttcga acgccacaat
     3061 ctccctggcg ttcgaacatt ccggcgtcga gtattctgga tgagctccat cgacatataa
     3121 acgtccgccg ttcgccaaca ccttgttcag ctgccggtta tagtcgggat tgggacgttc
     3181 tcgctcgccg tcgacttcaa atccccgggc atctaaaagt ggattttcgt tttcgtaatc
     3241 ccagagagct cccggagccg ggagtctcgg gtaatgtccg atcacggcga atgagcccga
     3301 cacagggtct accccagatg gatcttttgc cgcgatgccg aactcggttt cagtgccaag
     3361 cacgcgcatg cgattcgtgg atgtatgatc tggcatagga tagttagaga tagtggccgg
     3421 tactgatggt ttcaatctgc cgatgttcgg cgggcccacc actgatggtc cgaatatgaa
     3481 tgattttctc acccttcttt ccggcgactt tcgcccaatc gtcaggattg gtcgtattgg
     3541 ggagatcctc gtgttccttg aactcttcac ggatggcgcg gatcaagtcg tccgagtgca
     3601 gaccgactcc ctcttgcgcg atcacgcgct tcacggcgaa ttttttcgca cgagaaacaa
     3661 tcccttcgat caaggctccg ctggcgaagt ccctgaagta caggatttcc ttttcgccgt
     3721 tggcgtaggt tacttcgatg aacttatttt cttcactggc cgcatacatc gtctcgaccg
     3781 tcagagccgt caatcgatcg accagggcct gtcgatcgcc tccatgccgt tccaagtcct
     3841 cggcggcaaa aggaagatcc gtcgagatgt acttcgagaa aatgtcacgg gcagctgtgg
     3901 catccggtcg ccccactttg accttgacgt ccaaccggcc ggcacgaagc accgccggat
     3961 cgatcaaatc ttggcggttg ctggccccaa taacgatgac gttgcgcaat cgttcgaccc
     4021 cgtcgatctc tgaaagaaac tggggaacga tcgtcgactc gatatcggag gatatgcctg
     4081 tcccacgggt tctgaagagt gcatccatct catcgaaaaa cacgatcacc ggatgaccgt
     4141 cttcggcccg ttccttcgcc ttcttaaaca cctcgcgtac ctgacgctcc gattccccga
     4201 catacttatt gagcaactcg gggcccttca catgaagaaa gtagctccgg atttcttttc
     4261 ccgaccgatg gcctagtttc ttggcgatcg aattggcgac ggctttggcg atcagcgtct
     4321 tcccacagcc gggcgggcca tagagcagaa cccctttcgg cgcactgagc ttatactcgg
     4381 agaatatctg cggatgcagg aacggaagtt cgacggcatc gcggacttgc tccaattcct
     4441 tctggaggcc cccgatgtgc tcatagtcga catccgggac ctcttccaat acgagctctt
     4501 ccgcctcgga cttcggtaac ttctcaatga catagccgga acgagggtca tacaacagat
     4561 ggtcacccac actcagccgt tcagcgagca gtggctctcc gagttcggcc accttctcct
     4621 catcgaaatg gagcgtgacc aatgcccgtc ctccttccag cacgtctttg agtcgaacga
     4681 cctccccttg actgtcgaac cctttgactt cgatcacgtt gagcgcctca ttgagaacga
     4741 cttcctgtcc tttccgcaac tcattgcgct taatcgaagg gtgaaggctc accttcattt
     4801 tccgtcccga cacatagata tttccggtac cgtcagcatt catgctggaa aaaatggcat
     4861 aggccgacgg cggcgccgtc aatttttcga cttcggt
//
LOCUS       DATMTI010000415         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_652480, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000415 DATMTI010000000
VERSION     DATMTI010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652480"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1418
                     /locus_tag="VN039_06770"
     CDS             84..1418
                     /locus_tag="VN039_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWV45711.1"
                     /translation="MSRSSRISQFTKQDLWAGDLTTMSLFQRLGTQALRLATAVGLEF
                     RHRLLDARAASLVYTTLLSLVPFLAVMFSVLKGFGVHHVIEPFLAQALDPLGPKSKEI
                     TATIIGFVDNIKIGVLGVFGIGGLFYTTYSLIDKIEQALNAIWQVKQGRTWERKFADY
                     LSAVLVGPVLIFSAFGLLASLQSNTLVQHLAAMEPFGTLLVWSGEVVPLLMLCGVFTF
                     FYKVIPNTHVQIRSAAVGGVSAAILWIIVGEAFAKFVAASANYSAIYSSFAVLMLFLL
                     WLYTGWMIVLIGAQFSFFHQYPTAYLSRLLWEQGTDVFREQLALKVLRVLGYHYLKGD
                     RPLKLPELSSELNMPLSLVEEEVERLIENGFVGRLQEPQGVSLIKSPDLIFVKEVLDS
                     VHNGTPPWVLPHLGTNDPVSALLHRRDMAVERVLAGETVQSLLQDQPPSPIH"
     gene            complement(1550..1909)
                     /locus_tag="VN039_06775"
     CDS             complement(1550..1909)
                     /locus_tag="VN039_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWV45712.1"
                     /translation="MGADEVVLKPRVPADQIEQAKTWKTPLQQTQETIEKGKQLFHGK
                     AFCITCHRRDGKGLGDDIEPGTLKGPLPRDFTDKDWQAARTDGELFWILKNGSKGTAM
                     APFILTEEEAWQVVLYV"
     gene            complement(2072..2590)
                     /locus_tag="VN039_06780"
     CDS             complement(2072..2590)
                     /locus_tag="VN039_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HWV45713.1"
                     /translation="MSKSLSAFVAMAAVMCSGIAVQKALGGEPTIPAIGYDIHVQAPH
                     MMPDGTQGGPYHHYCKGISDKILQCLLFESTDPKAPLVGIEYFVAKDLTRKLPAIQWH
                     RHFHDHKVEIATGRVQVLDMPPDQAAKVAEAAAGTDGVIYHLWQHGLEFPDGTVSFPQ
                     SLGHKFPGYSDK"
     gene            complement(2745..3509)
                     /gene="ricT"
                     /locus_tag="VN039_06785"
     CDS             complement(2745..3509)
                     /gene="ricT"
                     /locus_tag="VN039_06785"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HWV45714.1"
                     /translation="MEPTLTEEFSHAYPESVRLVGVKVRNRGEVKKLDSGGMPLRTGD
                     PVLIEVEGEVTYGIVYLEPYSTPFIPPMRAMKSILRKPNTEETALIARLERLSLEAAT
                     YCRTKAMELGIHLKLVEVYGAMDRRQLTFVYTADDRIDFRELVRDLARRFGGRIEMRQ
                     VGVREEARRIGGIDSCGLVLCCASFLTDVKPISAKQAKKLDLPTDDPRLLGVCGRLKC
                     CLMFEMMDAQGAIAPQAQQLITPTKPDSPSSSTLPA"
     gene            3707..3976
                     /locus_tag="VN039_06790"
     CDS             3707..3976
                     /locus_tag="VN039_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HWV45715.1"
                     /translation="MVRERKKMLRIPNQVVLPFGYRISVRQLSDAEMDKRDANADGIW
                     DDDTKTIYVRKRLPVTRRRYILAHELGHAWLDWQHRYMDDGKAST"
     gene            complement(3995..>4208)
                     /locus_tag="VN039_06795"
     CDS             complement(3995..>4208)
                     /locus_tag="VN039_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWV45716.1"
                     /translation="NTHYDPESKPSAPRWDMVDIRYVRKFARPLTLDELRKENKLKGM
                     VLLKKGSRLSVQPVTALEWKHTISLV"
BASE COUNT          908 a         1121 c         1145 g         1034 t
ORIGIN      
        1 cctgacgact tcttaacaac ttgctagcgt gttttggaca gtcacgaagt tttaaataac
       61 cctgcttagg gagagcaggc cccatgagcc gctcctcacg catctcccag tttacgaagc
      121 aggacctttg ggctggggat ctgacgacca tgtcattgtt ccaacggttg ggcacccaag
      181 cgcttcggct ggccactgcc gtcggcctgg agttccgtca tcgcctgctt gatgcacggg
      241 cggccagtct ggtctacacg acgttgttgt cgttggtacc gtttctggcc gtgatgttct
      301 cggtgctgaa aggcttcggt gtccatcatg tgatcgaacc tttcttggcc caggccctgg
      361 atccgctggg cccaaagagc aaggagatca ccgccaccat catcggcttc gtggacaata
      421 tcaagatcgg cgtgctaggt gtgttcggga ttggcggcct cttttatacg acctactcgc
      481 tgatcgataa gatcgagcag gccttgaatg cgatctggca ggtgaaacag ggacgcacct
      541 gggaacggaa gtttgccgac tatttgagcg ccgtcctggt gggaccggta ctgatcttca
      601 gcgcgttcgg cctgttggcc tcgctccaaa gcaacactct ggtccagcac cttgcggcaa
      661 tggagccgtt tggcacgctg ttggtgtgga gcggcgaagt ggttcccctc ttgatgctct
      721 gtggcgtgtt cacctttttc tacaaggtga ttcctaatac ccatgtccag attcgctcgg
      781 cggccgttgg tggcgtgtca gcggccatcc tctggatcat cgtcggggaa gcctttgcta
      841 aattcgtggc ggcttcagcc aattacagcg ccatctattc gagtttcgcc gtgctcatgc
      901 tgttcctgct gtggctctat accggatgga tgatcgtatt gattggggcg cagttctcct
      961 tttttcatca atatcccacg gcctatttgt cacgcctgct gtgggagcag ggcaccgacg
     1021 tgtttcggga gcaactggct ctcaaagtat tgcgagtttt ggggtatcac tacctcaagg
     1081 gcgatcgtcc attaaagttg ccggaactat cgagcgaact gaacatgccg ttgtctctgg
     1141 tggaagaaga agtggaacgc ctcattgaaa acgggtttgt cggccgactt caggagccgc
     1201 aaggggtgag tctcatcaag tcaccggact tgatctttgt gaaggaggtt ctggattcgg
     1261 ttcacaacgg aaccccgccg tgggtcctgc cccacctcgg taccaatgat ccggtgtctg
     1321 ccctcttaca ccgtcgggat atggcggtgg aacgtgtgtt ggcgggtgaa accgttcaat
     1381 cgctgctaca agaccagccg ccgtccccca tccactgagc cttgatcgta tatgagatag
     1441 ttgtgtcacc gccggccgtt ttaagctgcc atttaactcc gtctttaggt cagaccgaaa
     1501 ccccttacgg ctgcctatct gttttcttgt cctgatcctg gccgaacgat cacacgtaga
     1561 gcacgacctg ccaagcttct tcttcagtga gaatgaacgg tgccatcgct gtgcccttgc
     1621 tgccgttttt caaaatccaa aagagctcac catccgttct cgcagcctgc caatccttat
     1681 cggtgaaatc gcgcggcaag ggacctttga gagtgcccgg ttctatatcg tctcccaatc
     1741 ccttgccgtc tcttctgtgg caggttatac aaaaggcctt cccatgaaag agctgtttcc
     1801 ccttttcgat tgtctcctga gtctgctgta ggggagtctt ccaggttttg gcttgctcga
     1861 tttggtcggc cggcacccgg ggtttgagta cgacttcatc tgctcccaac gcagaaagcc
     1921 tccctgaggc gagaattatc gctgtcaata gcgctggata gcgatcggga cagtccgctt
     1981 cctctcatgt cggccttgta cagtcatggg ggctgaccgt aatccccgca gctctatcgg
     2041 gtgaagacac gacgacttcc ccactgtcag cttacttgtc ggaataccct gggaacttgt
     2101 gaccgaggga ttgtgggaag ctcaccgtgc catcggggaa ttccagccca tgctgccaca
     2161 gatgatagat cacaccgtcc gtccctgctg cggcttccgc aaccttggcg gcttggtcag
     2221 gaggcatatc aaggacttgg actcggcctg tcgcgatctc tactttatga tcgtgaaaat
     2281 ggcggtgcca ctgaatggcg ggaagcttcc tggtgaggtc tttggcgacg aaatactcga
     2341 tcccgaccaa gggtgctttt ggatcggtcg attcgaagag cagacattga agaatcttgt
     2401 cggaaatgcc tttgcagtaa tgatggtagg ggccgccctg tgttccgtcc ggcatcatgt
     2461 gaggcgcttg gacatgaatg tcgtatccga tggctggaat tgtcggttcc ccaccgagcg
     2521 ccttttgaac ggcaattcca ctgcacatga ccgcagccat cgcgacaaat gctgacaaac
     2581 tcttgctcat tgcaacaaac ctccttggcc tgaggagttc tcgtctagcc tatctaggta
     2641 gagaatgtct ccacattcga ttgaccatta gagatgcctc agtaacaaag gattcgaata
     2701 ttctctgtaa gctcggataa cagtaaaaag ctgcgcggct acggttaagc gggtagagtc
     2761 gacgaggatg gagaatcggg tttggtcggt gtaatgagtt gttgggcttg aggggcgatc
     2821 gccccttggg catccatcat ttcaaacatc aaacagcact tcagccttcc gcagacgccc
     2881 agcaagcgag gatcatcggt cggcaggtcg agcttttttg cctgcttggc cgagataggt
     2941 ttcacatcgg tcagaaaact cgcacagcac agcacgaggc cgcaggaatc gatcccgcca
     3001 atcctccttg cctcttcgcg caccccgact tgacgcattt cgatgcggcc accgaaacga
     3061 cgtgccaggt ctcggaccag ttctcgaaaa tcgatccggt catcagccgt gtaaacaaag
     3121 gtcagttgtc gacgatccat ggccccatag acctcaacca gcttgagatg gatgccaagt
     3181 tccatcgctt tcgttcggca ataggtggca gcctccaggg agagccgctc aagcctggcg
     3241 attaacgcag tttcttcagt attaggcttg cgcaatattg acttcatggc ccgcatgggc
     3301 ggaataaagg gtgttgagta gggctctagg tagacaatcc catacgtgac ttcaccttcc
     3361 acttcgatca agaccgggtc accggtgcgc aatggcatac cgcctgagtc caatttcttg
     3421 acctctcccc ggtttctgac cttcactcca actaggcgaa ccgactcggg ataagcatgg
     3481 gagaactctt cggtaagggt aggttccata ggtcgatcat acacaaattt atgcgatagg
     3541 ggaacagttg ctaaagggac aacgatcgaa gtgcccgaac ctattgcaag agacgagaaa
     3601 cccataaagc atggataaaa catgagaaaa tggtcaacac tcgacctgcc ttgttatggt
     3661 aggctaagtc agcggtggga ggaagatggg gtgaacgatg gggacaatgg tcagagaacg
     3721 aaaaaagatg ttacgcattc cgaatcaggt tgtccttccg ttcggctacc ggatttcggt
     3781 tcgacagtta tccgatgcgg aaatggataa gcgagatgca aatgccgatg ggatttggga
     3841 tgatgacacg aagaccatct acgtacggaa gcgacttcct gttactcgcc gtcgatatat
     3901 tctcgcgcat gaactgggtc acgcctggct ggactggcaa catcggtata tggacgatgg
     3961 aaaggccagt acataaccgg taagcaacta ggccctagac taggctgata gtgtgcttcc
     4021 actccagggc ggtgacgggc tgaactgaga gacgagatcc cttcttcaac aacaccattc
     4081 ctttcagctt gttttccttc cgcaattcgt ccaaggtcaa tggacgagca aatttccgaa
     4141 catacctgat atcgaccatg tcccacctgg gagcggatgg tttgctctcg gggtcgtagt
     4201 gtgtattc
//
LOCUS       DATMTI010000416         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_313623, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000416 DATMTI010000000
VERSION     DATMTI010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313623"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VN039_06800"
     rRNA            complement(<1..1272)
                     /locus_tag="VN039_06800"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1627..>1892)
                     /locus_tag="VN039_06805"
     rRNA            complement(1627..>1892)
                     /locus_tag="VN039_06805"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          370 a          623 c          455 g          444 t
ORIGIN      
        1 tggccgggca gatgtgtcaa tgccacagct tcggtggtgt gcttgagccc cgctacattg
       61 tcggcgcgga atcacttgac cagtgagcta ttacgcactc tttaaagggt ggctgcttct
      121 aagccaacct cctggttgtc aatgcaactc cacatccttt cccacttagc acacgcttag
      181 ggaccttagc tggtggtctg ggctgtttcc ctctcgacta cggagcttat cccccgcagt
      241 ctcactgcca cgctctcact taccggcatt cggagtttgg ctaacgtcag taacctggtg
      301 aggcccatta gctatccagt agctctacct ccggtaagaa acacgtgacg ctgcacctaa
      361 atgcatttcg gggagaacca gctatcacgg agtttgattg gcctttcacc cctacccaca
      421 gctcatcccc tcagttttca acctaagtgg gttcggtcct ccacgacgtc ttaccgtcgc
      481 ttcaacctgg ccatgggtag atcactccgc ttcgggtcta gacccagcga ctaaatcgcc
      541 ctgttcggac tcgctttcgc tacggcttcc ccacacgggt taacctcgcc actgagcact
      601 aactcgcagg ctcattcttc aaaaggcacg ccgtcacccc acaaggaggc tccgacggat
      661 tgtaggcaca cggtttcagg atctatttca ctcccctccc ggggtacttt tcacctttcc
      721 ctcacggtac ttgtccgcta tcggtcacta gggagtattt aggcttagca ggtggtcctg
      781 ccagattcat acgggatttc tcgggccccg tgctacttgg gatactgctc aggagttcac
      841 gccatttcgt ctacgggggt agcaccctct gtgccgggcc tttcaatgcc cttcgactat
      901 aacgcgaatt tttgactccc tgccggcctg tcagaaccag ctgagcagtc ccacgacccc
      961 ttacctgcaa cgcctgacag ctatcacaca gataaggttt agcctcttcc gctttcgctc
     1021 gccactactc acggaatcgc ggttgctttc tcttcctgtg ggtactgaga tgtttcactt
     1081 ccccacgttc cctctactca ccctatgtgt tcagatgaga gtgactggac ttgcctccag
     1141 ccgggtttcc ccattcggaa atcctcggat cacagtctgg ttatcgactc cccgaggctt
     1201 atcgcagatt cctacgtcct tcttcggttc ctggtgccaa ggcatccacc gtgtgccctt
     1261 aaaaacttga gccacaaaga tgctcgcgtc cactgtgtag ttctcaagaa acgggcggcc
     1321 ccctgtcgaa cccggtgcct accagccaac aggccagcgg ttcatccgcg acaagggtcc
     1381 aagccagtcc aagaccggcc cgaaggtcaa gctctcgccc gagccttcag gacccaacaa
     1441 cgtgccaggc accaccacca cccgcaccgc gttccactgc caggccccgc gaacaggacc
     1501 ggcttgtact aacgagcacg aacagtgcca gcgccaacta atcgatgttc cacccttgag
     1561 cacccgctgc cccacactcg gggacagacc gggctctgga tcgcttgcgc gaccagtgct
     1621 ccttagaaag gaggtgatcc agccgcacct tccggtacgg ctaccttgtt acgacttagt
     1681 cccaatcgcc agtcccacct tcgacagctc cctcccacaa ggggttgggc caccggcttc
     1741 gggtgttacc gactttcgtg acttgacggg cggtgtgtac aaggcccggg aacgtattca
     1801 ccgcagcgtt gctgatctgc gattactagc gactccgact tcatggggtc gagttgcaga
     1861 ccccaatccg aactgagacc ggttttttgg ga
//
LOCUS       DATMTI010000417         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_483253, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000417 DATMTI010000000
VERSION     DATMTI010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483253"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..848)
                     /locus_tag="VN039_06810"
     CDS             complement(3..848)
                     /locus_tag="VN039_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45717.1"
                     /translation="MARWRPRRTVRVHSRWHDIPSLAVVWKRHLPQEPSRQLSDNWSL
                     LHGHCADHSSHLRLGRLLHRLDHHDLGVQESALAELELATQLIRVGLQVTFLPESQAR
                     TADLECRVGSERFFVEVTAMVGSAERRRLPMRGIMRDEEPEEETDRSVILTHRVLARI
                     SQKAKQLVDYCDPVILSISIPRADLRGGRQGRREQIRMDLKALAGSITVDLMRLRHLS
                     AVLISLWDVEPLPAKAGTRLANVDLVERTRHQRTHPRVRMFIQNPAAAASLSERQADI
                     VQRIL"
     gene            complement(902..1234)
                     /locus_tag="VN039_06815"
     CDS             complement(902..1234)
                     /locus_tag="VN039_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45718.1"
                     /translation="MNDHELHRVVQYVTASTSYARDTVSDILHTGLGELSSLAAHSTR
                     PFEREALLEYVSQWTIKRTGQPEPLVREVLGCAGRWLDEVYDECHAPPPEVLGESPDG
                     DEGAEPVP"
     gene            1389..>2229
                     /locus_tag="VN039_06820"
     CDS             1389..>2229
                     /locus_tag="VN039_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HWV45719.1"
                     /translation="MIARRPVRHKTSSPLMSDRWDLTHLVKNPLNDLERYLKALDAQV
                     VQVESARPQLQATMANSDFRSILALTESIVESSARLGAFAYLWFSEDTQNTKARSFKS
                     QVEERLTAVHNRLLFLDLWWQGVDNENAARLMADAGDLRYYLETIRRYTPHTLSESEE
                     KIINVKNVTGRSAVNTLYDVMTNGLTFTMKIGGKARTMNREELMSHVRNPKASIREAA
                     YRELYRVFSAQRDLLGEMYRTLVNDWKAENVGLRGFASPIATRNLGNDVPDQAVDVLL
                     ATCA"
BASE COUNT          488 a          673 c          599 g          469 t
ORIGIN      
        1 catcagagaa tccgctgaac gatatccgct tgccgctcgc tcagcgacgc agcggcggcg
       61 gggttctgga tgaacatgcg cacacgcggg tgtgttcgct gatgcctggt tcgctccacg
      121 agatccacat ttgccagcct agttccggcc ttcgccggca acggctcgac atcccagagc
      181 gaaatcaaca cggcgctgag gtgccggagt ctcatcagat cgaccgttat ggaaccagcc
      241 aacgccttca aatccattcg aatctgctct cgacgcccct gccttcctcc ccggaggtcg
      301 gctcgcggta tcgagatgct caagatgacc ggatcgcaat agtcgacaag ctgctttgcc
      361 ttttggctga tgcgcgccaa gactcggtga gtcaagatca cactgcgatc cgtttcttct
      421 tcgggctcct catctctcat gatcccgcgc ataggcagcc gccgtcgctc agccgaacct
      481 accatcgccg tcacttcgac aaagaaccgt tccgatccca cacgacattc caaatcggcc
      541 gttctggctt gcgactcggg tagaaacgtg acctgaagtc cgacgcgaat cagctgcgtg
      601 gccagctcca gttccgccag cgcagattcc tgcacaccga gatcatgatg gtccaagcgg
      661 tgaagaagcc gtccgagccg gagatggctt gagtgatcgg cacagtgccc atgcaggaga
      721 ctccaattat cactgagttg gcgtgaaggc tcctgtggta aatgacgctt ccaaacaacc
      781 gcgagactgg ggatatcgtg ccagcgggaa tggactctca ctgtgcgtcg tggccgccag
      841 cgtgccatca ctttctccat tccggctcat gaagcgtcgc ttcacgagcg acccaagtca
      901 tttatgggac cggctcggcg ccttcgtcgc catccggtga ttcgcccaag acttccggtg
      961 gtggagcgtg acattcatcg tacacttcat ccagccaccg ccccgcacag cccaggactt
     1021 cccgcaccaa cggctccggc tggcctgttc gtttgattgt ccattgggaa acatattcga
     1081 gaagcgcttc ccgctcgaag ggtctggtcg aatgcgcggc cagggaagac agctctccga
     1141 gacctgtgtg aagaatgtcc gacaccgtgt cccgagcata cgatgtactc gcggtcacat
     1201 attgaacaac gcgatggagt tcgtggtcgt tcatgttgca gtccgtgaaa ataatcttta
     1261 caccccaatg actgactgtc aatgcggccg gacttccttt gattccgatg attttctcca
     1321 atcacagcat agcctaatga taagatgcat ctgctcaccg tttggctacc acgcaaagga
     1381 gattcggcat gatcgcgaga cgtccggttc ggcacaagac ttcatccccg ttgatgtctg
     1441 atcggtggga cctcacccat ctcgtcaaga accccctgaa tgacttggag cggtatttga
     1501 aagcgcttga tgcacaagtc gtacaggttg agtcggctcg tcctcagctc caggcaacaa
     1561 tggcaaactc tgatttccga tcgatcctgg cgctcaccga gtcgatcgtg gagagctctg
     1621 cccggcttgg cgcattcgcc tacctctggt tttcggaaga tacccaaaac accaaggcgc
     1681 gctcgtttaa gtcgcaggtg gaggagcggc tcaccgccgt acacaatcgc ttgctctttc
     1741 tcgacctctg gtggcagggg gtagataacg aaaatgcggc gaggctgatg gccgacgccg
     1801 gcgaccttcg gtactacctg gagaccatca gacgttacac acctcataca ctttcggaat
     1861 cggaagagaa gatcatcaat gtgaagaatg tgaccggccg gagcgccgtc aacacactct
     1921 atgacgtcat gaccaacgga ctgacattca cgatgaagat cggcgggaag gcacgcacca
     1981 tgaaccgcga agaactcatg agccatgtgc ggaacccaaa ggcatccatt cgcgaggctg
     2041 cctacagaga actctatcgc gtgttctcag cccaacgtga cctgctcgga gagatgtatc
     2101 ggacactcgt gaacgactgg aaggctgaga acgtcggact ccgggggttt gcttctccca
     2161 tcgcaacccg caacctcggc aatgatgtgc cggaccaagc cgtcgatgtg ctgttggcga
     2221 cctgtgcca
//
LOCUS       DATMTI010000418         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_143420, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000418 DATMTI010000000
VERSION     DATMTI010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143420"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VN039_06825"
     CDS             <1..363
                     /locus_tag="VN039_06825"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWV45720.1"
                     /translation="SEEARHAMWAERHREKTGILAALKSQGLLPPGVSENPAQTPTMT
                     TGLMEGIHQYLARTPAWIVLANIEDVIGTRCQANLPGTVDQHPNWCRKLGLTVEDLIQ
                     DSRFERLASLLRLTRPLV"
     gene            443..1615
                     /locus_tag="VN039_06830"
     CDS             443..1615
                     /locus_tag="VN039_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV45721.1"
                     /translation="MTAITHRDRLVLVIAASCFLIILVIEEFAPVNVVGAYGYVLPIL
                     LVTTLRNRTIMLVTVLACVVATYSGLLQPTKPGRFQTAVINRSVVVGVLLLVAYLGVS
                     WEERKAREEATRAALARETENLLKANTQLVQAKDQLNRSERLAAVGQLVASVAHEVGT
                     PLHSIAWHVQALAEEPGATPEMRKRVAIIDDQITRVVRIIQDLLSSTRPRQPEPQWLP
                     VEEVVGPSAILMESAFHEKGIVLAVDIPKDLPLIWADKEKIHQVLVNVLTNALAATPS
                     QGKVTIMAGSRKASSDELDRGRLVAHGMSPVVITIEVQDTGCGMPEEDARKAFEPFFT
                     TKAVGKGTGLGLFLSRESVVAHGGSLALTSESGCGTAVTMTLPAMRREAIQTREEA"
     gene            1615..>2873
                     /locus_tag="VN039_06835"
     CDS             1615..>2873
                     /locus_tag="VN039_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV45722.1"
                     /translation="MQPTTILVADDDAVARELLAEALRKEGYHVEAFAGGEEVIARGR
                     QGRVDLVLTDIRMGAVDGLTVLREFKRVSPNTAVVVLTAFGSLEGAIEAIKQGAYDYL
                     AKPFKKEDIKLVVKRGLDHCRLLQENARFREELKNKGEWSPLVGSSTAMLEVYKLVAR
                     VAESKSTVLLQGESGTGKELIARAIHTNGSRRDKPFIPVNCGALPDTLLESEMFGYEK
                     GAFTGAAGPKVGLFESANGGTLFLDEIGELGQALQVKLLRVMQDQEVRRVGGTTSTRV
                     DVRIIAATNRDLEQLVKEGKFRDDLFYRLKVVPITLPSLLDRREDIPMLVHHFLQKCA
                     GGTDHAVRGVLPETMALLIQYRWPGNVRELENAIERAVSLSHGPLLTPEDLPEVIRQN
                     ATGEVDARSAQVDQLDEVCLTLEEVEKR"
BASE COUNT          628 a          696 c          933 g          616 t
ORIGIN      
        1 tcggaggagg ctcgacatgc gatgtgggcg gagcggcatc gagaaaagac agggattctc
       61 gccgcattga agtcccaagg gctcttgccg cctggtgtct cggagaatcc ggcccagacg
      121 ccgacgatga cgactggatt gatggagggg attcatcagt atctggcccg tacccccgca
      181 tggattgtct tggccaatat cgaagatgtc atcggtaccc gctgccaggc caatctaccg
      241 ggaacggtgg accagcaccc caattggtgc agaaaattgg gtctgacggt ggaggatttg
      301 atccaagact cccgatttga acgccttgcg tctctgttgc gtttgacccg ccctcttgtg
      361 taagtacttt cgtgaaggaa cccactcatt atcgatgttc ttgattcaga agttgtgacc
      421 ctcggccggg cggagcgagt cgatgactgc cataacgcat cgtgaccgcc tcgtgcttgt
      481 catcgcagct tcctgttttc tcatcatctt ggtcattgaa gagttcgccc cggtcaatgt
      541 cgttggtgcg tatgggtatg tgttgcccat cttgttggtg accactcttc ggaacagaac
      601 tatcatgttg gtgacggtgc tggcctgtgt cgttgcgacc tattcaggtc tactccaacc
      661 gacgaagccg ggccgatttc aaaccgccgt catcaaccga agtgtcgtcg tcggcgtctt
      721 gctgttggtg gcatatctgg gtgtgagctg ggaagagcgg aaggcgcgag aggaggcgac
      781 gagggcggcg ctggctcgag aaaccgagaa tctacttaaa gccaacacac aactggtcca
      841 ggcgaaagat cagctgaacc ggtcggaacg gttggctgcg gtgggacagc tcgtggcctc
      901 cgtggcgcac gaggtgggca cgccgcttca ctcgatcgcg tggcatgtcc aggctctggc
      961 cgaggagcct ggtgccacgc cggagatgag gaagcgggtg gctatcatcg atgatcagat
     1021 cacgcgtgta gttcgaatta ttcaggattt attgtcttct acacgacctc gccaacctga
     1081 gccgcaatgg cttccagtgg aggaagtggt cggcccctct gcaatcctga tggagtcggc
     1141 gttccatgag aaagggatcg ttctggcggt tgacattcca aaggaccttc cgctgatttg
     1201 ggcggacaag gaaaaaatcc atcaagtatt ggtgaacgtc ctgaccaatg cgcttgctgc
     1261 aacgccctca caggggaaag tcactatcat ggcaggaagt cgcaaggcgt cgtcggacga
     1321 actggaccgc ggtcggctgg tcgcacacgg catgtcgccg gtggtaatca cgatcgaggt
     1381 gcaggacaca ggatgcggta tgccggagga agacgcacgg aaagcctttg aaccgttttt
     1441 tacgacgaag gcggttggca aggggaccgg cttggggcta ttcttgagcc gagaatcggt
     1501 ggtagctcat ggtggcagcc tggcgctgac gagtgagagc gggtgtggca cggccgtgac
     1561 gatgacatta ccggcgatgc gacgtgaagc cattcagacg agagaggagg cgtaatgcaa
     1621 ccaacgacga ttctggtggc cgacgatgat gcggttgccc gtgagctgtt ggcagaggct
     1681 ctcaggaagg aaggatatca tgtcgaggcc tttgccggtg gagaagaggt gatcgcgcgt
     1741 ggacgccagg ggcgggtgga tttggtgttg acggacatcc gcatgggcgc ggtggatggg
     1801 ctgacggtct tgcgggagtt caaacgagtg agtccgaata cggccgtggt ggtgctcacc
     1861 gcgttcggct ctctggaagg cgcgatcgaa gcgattaagc agggcgccta tgattatctg
     1921 gcgaagccgt tcaagaaaga agacatcaag ctggtggtca aacgggggct ggatcactgt
     1981 cgattgctcc aagagaatgc gcgatttcga gaagagttga agaataaagg cgagtggtct
     2041 ccattggttg gaagcagcac ggcgatgttg gaggtgtaca agttggtcgc gcgtgtggcc
     2101 gaaagcaaaa gcacggttct gctccaggga gaaagcggta ccggcaaaga gctcattgcg
     2161 cgggccattc ataccaatgg ctcgcgccgg gataagccgt ttatcccggt caattgcggt
     2221 gcgttgcccg ataccttgtt ggaatcagaa atgttcggat atgagaaagg cgctttcacc
     2281 ggcgcagcgg ggcccaaggt cgggctcttt gaatcagcca atggagggac gctgtttctc
     2341 gacgagatcg gtgagcttgg acaggcgtta caagtgaagt tgttgcgggt catgcaagat
     2401 caggaagtcc gccgggtcgg gggcacgaca tccaccagag tcgatgtccg gatcattgcc
     2461 gcgaccaacc gagatctgga gcaactggtg aaggaaggaa agtttcggga cgacctcttc
     2521 taccggttga aagtcgttcc aatcacgtta ccgtcgttgc tcgatcggcg agaagatatt
     2581 ccgatgctgg tgcatcactt cttgcaaaaa tgcgcgggag gcacggacca tgcggtacgg
     2641 ggagtcttgc cggaaactat ggcattgttg atccaatatc ggtggccagg caacgttcga
     2701 gagcttgaaa atgcgattga gcgcgccgtg tcattgagcc atggccctct cctgactcct
     2761 gaggacttac cggaagtgat tcgccaaaat gcgactggcg aagtcgatgc acgatctgct
     2821 caagtcgatc aacttgatga agtgtgtctg accttggaag aagtggaaaa acg
//
LOCUS       DATMTI010000419         1721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_16291, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000419 DATMTI010000000
VERSION     DATMTI010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16291"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VN039_06840"
     CDS             complement(<1..330)
                     /locus_tag="VN039_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV45723.1"
                     /translation="MNLVRLAIAGSLVVLQYACVAVNPPLSAPPESNATAAQHNSDGI
                     VHYEMGHWSIAKDHFASAVEADPNLAEAHYNLGLALDKLNLHSDATTHFTKAAELAPA
                     NSAIGESS"
     gene            complement(448..>1721)
                     /locus_tag="VN039_06845"
     CDS             complement(448..>1721)
                     /locus_tag="VN039_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV45724.1"
                     /translation="PQASIHTANTVSEGVALWSKISPDLIFGEGNAHTLAPLLEHARQ
                     LPVCPPLLVIGDSHSVKDAVEIMRAGATDYLPRPICIEDLQTAVTRAFGHPTSAPPSS
                     PQDPFTSIISISAQMNLMKRLAKEVAITDATVLITGESGTGKELFAEAIHRCSLRANG
                     PLITLNCAGIPENLLEAELFGYESGAFTDAKRSKPGRFQMAEGGTIFLDEIGEMSPTA
                     QAKLLRVLENHTIDPLGDIRSRKVNIRIIAATNEDLLAHIKAGRFRLDLYYRLNVYQL
                     RIPPLRERLEDIEPILLIFLERAKQERGCRIRTIAPAALAVLQSHDWPGNVRELHNVV
                     EWLTITCKAEIIQPAHLPASVKTAPPTNCLSQSSNPSLLAFGLSFQDMEKQMLEEALR
                     KSSGNVSEASRLLKMTRNTLRYRMAKYHLHE"
BASE COUNT          377 a          419 c          520 g          405 t
ORIGIN      
        1 gcttgactcg ccgattgcgc tgttggcggg tgccagttcg gccgcttttg tgaaatgggt
       61 tgtggcatcg gagtgaagat tcagtttatc gagtgcgaga ccaagattgt aatgagcctc
      121 ggcaagattc ggatcggctt cgacagcgga cgcgaaatga tccttggcga tcgaccaatg
      181 gcccatctca tagtggacaa tgccgtcgga attgtgctga gcggcggtgg cattgctctc
      241 cggcggggca gataagggcg gattcacggc gacacaagcg tattgcagga ctacaaggct
      301 tcctgctatg gccagtcgga cgagattcat ggttcacctc cagagtaaga atccatgcct
      361 gcgatggccg ggaagaccgg cggacgcaga ctgtgagtcc gaagcaaagg gaagcggccg
      421 catgcaaaat aactagacac ggttcctcta ttcgtggagg tggtactttg ccatacggta
      481 gcgcagggtg ttcctggtca ttttgaggag tcgactggct tccgagacat tccctgacga
      541 ctttcgcaac gcttcttcaa gcatctgttt ttccatgtcc tgaaacgaca gcccaaaggc
      601 cagcaacgac ggattggaac tctgactcag gcaattggtc gggggagcgg tcttcacgga
      661 tgccggtaaa tgagcaggct gaataatctc tgctttgcac gtgatggtca gccattcgac
      721 gacattgtgg agctcgcgta catttccagg ccaatcgtgg ctttgaagga ccgcaagagc
      781 tgccggggcg atagtcctaa tacggcatcc gcgttcctgt ttggcccgct ccaaaaaaat
      841 caagagaatc ggctcgatat cttcaagtcg ttcacgcaag ggtggaatgc ggagctggta
      901 gacattcaac cgatagtaca gatcgagacg gaaacgaccg gccttgatgt gggcaagcag
      961 atcctcattc gtggcggcaa tgatgcggat gttgacctta cggctgcgga tatctcccaa
     1021 gggatcgatg gtgtggtttt ctagtacgcg taggagtttg gcttgagcgg tcggactcat
     1081 ttctccgatt tcatcgagaa agatcgttcc tccctcagcc atttggaatc ggcctggttt
     1141 tgaccgcttg gcgtcggtga aggcacccga ttcataaccg aataactcag cctccaagag
     1201 attttctgga atccccgcgc aattgagcgt gatcagcggg ccgttcgctc gtaggctgca
     1261 tcggtggatg gcctctgcaa acaattcctt tccggttccg ctttcaccgg taatgaggac
     1321 ggttgcgtct gttatggcca cctctttggc cagccgtttc atcagattca tttgtgctga
     1381 gatgctgatg atggaggtaa atggatcctg tggagaagag ggtggagccg aggtaggatg
     1441 cccgaatgct cgagtgacgg cagtttgcag atcctcgatg cagatcggtc tgggtagata
     1501 atcggtggcg ccggccctca tgatctcgac agcgtctttg acggaatggc tgtccccaat
     1561 cacaagcaag ggaggacaga cgggtaattg tcgggcatgt tcaagcaggg gagccagagt
     1621 gtgggcattc ccttcgccga agatcagatc cggggaaatc ttcgaccata gagcgacgcc
     1681 ttccgatacc gtgttcgcgg tatgaatact ggcttgaggt g
//
LOCUS       DATMTI010000420         1915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_525661, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000420 DATMTI010000000
VERSION     DATMTI010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525661"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /gene="hisB"
                     /locus_tag="VN039_06850"
     CDS             <1..214
                     /gene="hisB"
                     /locus_tag="VN039_06850"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="catalyzes the dehydration of
                     D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to
                     3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HWV45725.1"
                     /translation="IKAFDLGLFEDFFQAFVTHGGLNLHVNLLYGRNPHHIMEAIFKA
                     LAKALDQATLPEERLAGKVLSTKGML"
     gene            complement(486..700)
                     /locus_tag="VN039_06855"
                     /pseudo
     CDS             complement(486..700)
                     /locus_tag="VN039_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943399.1,RefSeq:WP_011813231.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1104..1712
                     /gene="hisH"
                     /locus_tag="VN039_06860"
     CDS             1104..1712
                     /gene="hisH"
                     /locus_tag="VN039_06860"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWV45726.1"
                     /translation="MIAIIDYGMGNLRSVSKAFEAVGHQAIVTRDAATIQNASHVVLP
                     GVGAFGDCMINLQQYGLIEPIKTAIQSGKPFLGICLGFQLLFTESEEFGKHEGLDIFP
                     GNVRAFSKDQVLKVPHMGWNQVNIQSHCPVFKGIADGSHCYFVHSFFVDPRDKQITDT
                     TTTYDVPFTSSIWKDNVVACQFHPEKSQAVGLQLIKNFGFWK"
BASE COUNT          425 a          517 c          519 g          454 t
ORIGIN      
        1 gatcaaagcg tttgatctcg gcctattcga agatttcttc caagcctttg tcacccatgg
       61 cggtctgaac ttgcacgtga atctactcta tggccgtaat ccccaccaca tcatggaagc
      121 catcttcaaa gcgctggcca aggcactcga tcaagcgacg ttgccggaag aacggctagc
      181 cgggaaggtg ctctcgacga aggggatgct gtagttcgaa gacgctgtga ggaacaaggg
      241 ttaccaaaat ctctgttaag ctgaccacag agactgactc cgagcatctc attgatagca
      301 tctcagcggt ctatttttat cagaattcta acttgatttt ccccatgaac aagtggtccg
      361 gccgcatccg ggaccttcga gttgaacttc tcctccgagc atccataacg cctgtttgcg
      421 ccggggtggt ggctcatggt ggagcactcg tacgggccga tgccagtgca ctagggcgtc
      481 gggactcatg tcgccaccgt caccagattc cggatgcacc aggttatacg ccgccgcggc
      541 gaaagtgaaa cgccagccga cacgcggcac accgcggagc ttgactttgc ggagcagtcc
      601 tacggtcttc aaccagccgc agatctcttc gacccgtttg cgtgtctgtt cactgatcga
      661 cagagcccgg gtgtcgcgtg gtccgaccgt caatggtact cgtccaccgc gtcgggtacc
      721 gggcggcatg cgggtcacct gtcgctcccg caatgcgtcc acgaacgtga cgatggcatc
      781 gtgtttacag cggcagtcgg ataccgcccc acactgtgta atgcctttag gatctcctgt
      841 cgcatctcat tgaccccccg catagccgaa atagacctcg attttcaaac tctcggtggg
      901 gccctctcgt cctggccgac caagcatatg ctgccggtga gcgcctacat cagcgagaca
      961 atcggcgcac aacacagatt taccggattg acatcgctct gggtcaaatc gaatcgggca
     1021 gaccggaaaa ggctgatgtg agattgccag tggcaggaac cttccagttg cggtattgtt
     1081 acgactacct aagtggggac ggcatgatcg caattatcga ttacggaatg gggaatcttc
     1141 gtagtgtctc caaggccttt gaggcagtag gacaccaggc gattgtcacg cgcgatgcag
     1201 ccaccattca aaacgccagt catgtcgttc tgccgggcgt cggggcgttc ggtgattgca
     1261 tgataaatct gcagcagtat ggattgatcg agcccatcaa gaccgcgatt cagtcgggaa
     1321 agccgttctt ggggatctgt ctggggttcc agctgttgtt tacggaaagt gaagagttcg
     1381 gaaagcatga aggtctcgac atttttccgg gtaacgttcg agccttctca aaggaccagg
     1441 tcctcaaggt tccacacatg ggctggaatc aggtcaacat ccaaagccac tgtccggtgt
     1501 tcaaaggcat agccgacggg tcccattgct attttgtgca ttcctttttt gtggatccac
     1561 gggacaagca gattacagac acgaccacga cgtatgacgt cccctttacg tccagtatct
     1621 ggaaggacaa tgtggtggcg tgccagttcc atccggagaa gagtcaggcg gttgggttac
     1681 aattgatcaa gaatttcgga ttctggaagt gattatggtc gccgttcact cgcgtatgag
     1741 ctcgatgatg acctgccttc tattggcaat cgttgtcggc tctggttgca gtggaccaaa
     1801 ggtagcgacg aagtcgtcca ttgaactttc tcgatacaag atccgatcca ttgccttgat
     1861 gccgttcacg tcgatttcta cgccccaagc acgtgatcaa gacgaattct ttttt
//
LOCUS       DATMTI010000421         1627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_652707, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000421 DATMTI010000000
VERSION     DATMTI010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652707"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VN039_06865"
     CDS             complement(<1..182)
                     /locus_tag="VN039_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45727.1"
                     /translation="MGATTSGKRRETRRAPPPSSHIHREVIGVILIALSLLMLLSLLS
                     FVPGEARIVADGASGT"
     gene            complement(274..945)
                     /gene="phoU"
                     /locus_tag="VN039_06870"
     CDS             complement(274..945)
                     /gene="phoU"
                     /locus_tag="VN039_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWV45728.1"
                     /translation="MIQRHFDEELAELKTKLARMVGLAEDQIDKALAALVTRDSALAC
                     RVIERDHKVNALDVEIDEACIELLALHQPAARDLRLVTTAMKISTELERISDLSENIS
                     ERAIELNEEPQLKPYIDIPRMGNLARVMVKESIDAFVKEDSKLARKVLVDDDLVDDLM
                     EQLFRELLSFMMENPHTISRAIRLSFVAKYLERMADHATNIAELVVYLVEGKIIRHTS
                     PPVPL"
     gene            complement(950..>1627)
                     /gene="pstB"
                     /locus_tag="VN039_06875"
     CDS             complement(950..>1627)
                     /gene="pstB"
                     /locus_tag="VN039_06875"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWV45729.1"
                     /translation="EYKITALIGPSGCGKSTFLRCFNRMHDLYPGTCYEGEILLYPDG
                     RNILAREVDPIEVRMRLAMVFQKPNPFPKSVYENVAYGLKVRGVMDRRILDEKIEQAL
                     RSAALWEEVKDRLSAQATSLSGGQQQRLCMARALATDPELLLLDEPTSALDPIATANI
                     EELLLDLKRRVTILIVTHNMQQAARVSDWTAFMYLGELVEFGLTKQLFTNPSRKQTED
                     YITGRFG"
BASE COUNT          364 a          445 c          440 g          378 t
ORIGIN      
        1 ttggtgcccg atgcaccgtc cgccacaatt ctcgcctccc cgggcacaaa cgacagaagg
       61 ctcaagagca tgagtaagct cagtgcgatc aagatcacgc cgatcacttc acgatgaata
      121 tgagaagagg gaggtggggc acggcgggtt tctctccgct tacccgaggt ggtggcgccc
      181 atgcagtgga tgctagcaca gggggagggt catttcaaac aaatgggcat catgctgcgg
      241 tcgtgtgcgc gcctgaaaaa atgaatcgtg gcgttacaac ggaaccgggg gtgatgtgtg
      301 tcgaatgatc ttgccttcca ccagatagac gaccaactcg gcgatgttgg tcgcatgatc
      361 ggccatccgc tcaagatact tggcgacgaa gctcaaacga atagcacgtg aaatggtatg
      421 cgggttttcc atcataaagg aaagcagctc gcgaaataac tgttccatca gatcatcgac
      481 cagatcgtca tccacgagca ctttccgagc gagcttggaa tcttccttga cgaaggcgtc
      541 gatgctttcc ttcaccatca ctcttgcgag gtttcccatc ctcggaatat cgatgtacgg
      601 cttgagctgc ggttcttcat tcagctcgat cgcgcgctcc gagatattct ccgagagatc
      661 gctgatccgc tcaagctcgg tggagatttt catggccgtc gtgacgagcc gtaaatcgcg
      721 cgcggccggt tggtggagcg ccaacaactc gatacaggcc tcgtcgatct ccacgtcgag
      781 tgcattcacc ttgtggtctc gttcgatcac gcgacaggcg agggccgaat cgcgagtaac
      841 caaggcagcc aatgccttat cgatttggtc ctctgccaga cccaccattc gtgcgagctt
      901 ggtttttaat tcggcaagtt cttcgtcgaa gtgtcgctga atcataagcc taaccgaatc
      961 gtccggtaat atagtcttcg gtctgtttcc ttgatggatt cgtgaacagc tgcttcgtga
     1021 ggccgaactc gacgagttca ccaagataca tgaaagccgt ccaatccgac acccgcgccg
     1081 cttgctgcat gttatgcgtc acgatcagaa tggtcacgcg tcgcttgaga tcgagcaaca
     1141 gttcttcgat gttggccgtg gcaattgggt cgagcgccga cgtcggctca tccaggagca
     1201 gcagttccgg gtcggtcgcc agggcgcggg ccatacagag acgttgctgc tggccgccgg
     1261 atagggatgt tgcttgtgcc gacaatcgat ccttgacctc ttcccataat gccgcacttc
     1321 gcaacgcctg ttcgatcttc tcatccaaga ttcgccgatc cataactccg cgaaccttta
     1381 acccataagc aacattttca tagacggatt tggggaaagg attgggtttt tgaaatacca
     1441 tggccaaccg catccgtacc tcaattgggt ccacctcgcg agccaggatg ttgcggccat
     1501 ccgggtagag caagatttct ccctcgtaac aggtccccgg gtagagatcg tgcatccggt
     1561 taaaacaacg gaggaaggtg gattttccgc aaccagacgg tccaatcaat gccgtgattt
     1621 tgtattc
//
LOCUS       DATMTI010000422         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_440375, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000422 DATMTI010000000
VERSION     DATMTI010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440375"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..822)
                     /gene="trpA"
                     /locus_tag="VN039_06880"
     CDS             complement(19..822)
                     /gene="trpA"
                     /locus_tag="VN039_06880"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWV45730.1"
                     /translation="MSGRLESTFDGLRQRGQKALIAYLMAGDPGLAETEQLVLALEQA
                     GADIIELGVPFSDPIADGPVIQQAAERALRNGTSLRKVLASVTSLRQRTNIPIILMLY
                     YNSIHAMGCEEFCHAAKAAGVDGLIVPDMPPDEAGPLKMPADAAGLSLIFLLAPTSTN
                     DRRKLVAKESHGFVYYVSLTGITGAKINNIGDIQYNIGKLRKVSAAPVAVGFGVATPE
                     DASRVSKMADGVIVGSAIVKRIASHQQDPAMVGHVAEFVRSLKAAMAPA"
     gene            complement(819..2012)
                     /gene="trpB"
                     /locus_tag="VN039_06885"
     CDS             complement(819..2012)
                     /gene="trpB"
                     /locus_tag="VN039_06885"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWV45731.1"
                     /translation="MPMLPDSHGRFGSYGGRYVPETLMPALLELEEEYAEARRDRRFQ
                     TDLAYYLKQYVGRPTSLYRADRLTKKLGGAKIYLKREDLCHTGAHKINNAIGQVLLAL
                     RMKKRRIIAETGAGQHGVATATAAAMFGLQCEIYMGTEDMQRQALNVFRMRLLGATVT
                     GVDAGSRTLKDAISEAMRDWTTNVQTTHYVLGSVLGAHPYPMMIRDFQAIIGREARKQ
                     ILSAEKRLPDYLVACVGGGSNSIGLFHAFLRDPKVKMVGVEAGGLGVESGKHAARFSG
                     GKPGVLQGTMTYLLQDENGQINLTHSVSAGLDYAAVGPEHSLYHDQGRIEYTYATDSE
                     ALAAFDLLAREEGIVPALESAHAIAHVVKLAPKLKKSQLIIANLSGRGDKDVQQVARM
                     RGVSL"
     gene            complement(2060..2686)
                     /locus_tag="VN039_06890"
     CDS             complement(2060..2686)
                     /locus_tag="VN039_06890"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWV45732.1"
                     /translation="MKIKICGITNVKDANVAVKAGADALGFVMYRKSPRWVEPAVARS
                     IIAGLPPFVLAVGVFVNEEAEKVRTLMDECGFVLAQLHGDESALYCQNLGRPALKALR
                     LKDRGTFLALAEFQGRANVRGVLIDAFSDQTYGGTGQTVDWTLAQEAARSTPVILAGG
                     LNPQNVAGAIQIVRPYGVDVSSGVELSPGKKDHHKLKAFIEAARLVSV"
     gene            complement(2697..>3341)
                     /locus_tag="VN039_06895"
     CDS             complement(2697..>3341)
                     /locus_tag="VN039_06895"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWV45733.1"
                     /translation="SPSLGLLREEFSEQFDYLGLARTYHEHGASAISVLTDKDFFQGD
                     LRYLKEIKHALPIPALNKEFMVGDVQFYEARAHGADAVLLIVAALERRQLMDFHALAS
                     ELGMDSLFEAHHEKELDTVLEWIPNARMIGINNRDLNTFTTDLNVTFRLAKRIPSDKL
                     IISESGIHDRDAVTRLTEAGVHAMLIGESLIRAEHTADKVRELLGQAAGRKGAS"
BASE COUNT          700 a         1023 c          886 g          732 t
ORIGIN      
        1 gtgtgaccga acagctagct aggccggcgc cattgcagcc ttcaaagatc ggacgaactc
       61 ggcgacgtgt ccgaccatgg cgggatcctg ttggtgggac gcgatacgtt tgacgatggc
      121 acttccgacg atcaccccgt ccgccatttt cgatacccgc gaagcatcct cgggtgtcgc
      181 cacgccaaag ccgacagcga cgggggcggc ggaaaccttt cgcaatttgc cgatattgta
      241 ttgtatgtcc ccaatgttgt tgatcttcgc ccctgtaata cccgtcagtg agacataata
      301 gacaaacccg tgcgactctt tcgccacaag ctttcgccgg tcattggtgc tggtcggcgc
      361 gagcaagaag atcagcgaga gtccggctgc gtctgccggc atcttgagcg gtcccgcttc
      421 atccggcggc atgtcgggca cgatcaggcc gtccactccg gcagccttcg ccgcatggca
      481 aaactcttca cagcccatgg cgtggatgga attgtaatac aacatcagga tgatcgggat
      541 attcgtccgt tgcctgagcg atgtgacgga ggccaggact ttccgaagcg acgtgccgtt
      601 ccggagcgca cgctccgccg cctgctgaat caccggtccg tccgcgatcg gatccgaaaa
      661 gggcacgccc agctcaatga tatcggcccc cgcctgttcc aacgccaaga ccagttgttc
      721 tgtttcagcg agtcccggat ctcccgccat caggtaggca atgagcgcct tctgccctct
      781 ctgacgcaga ccatcaaacg tggattccag ccgtccgctc acaatgacac acctcgcatt
      841 cgcgcgactt gctgtacgtc cttgtcaccg cgacccgaaa ggttggcgat aatgagctgc
      901 gacttcttga gcttgggagc cagcttgacg acatgcgcga tggcatgggc gctctcgagt
      961 gccggaacaa tgccttcttc acgcgcgagg agatcaaaag ccgccagcgc ctcgctgtct
     1021 gtcgcatagg tatattcgat gcggccttga tcatggtaga gactgtgttc cggtccaaca
     1081 gccgcataat ccagaccggc cgacaccgaa tgggtcaagt tgatctgtcc gttttcgtcc
     1141 tgaagcaggt aggtcatggt gccttgcaag acccccggct tgccgcctga aaatcgagcc
     1201 gcatgttttc cactctcgac cccaagtcct ccggcttcca cgccgaccat cttgaccttg
     1261 gggtcgcgga ggaaggcatg gaaaaggccg atcgagttgc tgcctccccc gacacaggcc
     1321 acgagataat ccggcaaccg tttttccgcg gaaagaatct gcttccgcgc ttcccgtccg
     1381 atgatcgcct gaaaatcgcg gatcatcatg ggatacggat gggctcccag gaccgacccc
     1441 aacacatagt gcgtggtttg tacgttggtc gtccagtcgc gcatggcctc gctgatggca
     1501 tccttgagag tgcggctgcc ggcatcgacg ccggtcaccg tcgcacccag cagacgcatg
     1561 cggaagacgt tcagcgcctg ccgctgcata tcttccgtac ccatgtagat ctcgcattgc
     1621 aaaccgaaca tggcagccgc cgttgcagtc gccacaccgt gctgaccggc ccccgtttcg
     1681 gcgatgattc gccgtttctt cattcgcaag gccaggagga cttggccgat cgcattgttg
     1741 atcttgtgcg cgccggtatg gcagaggtct tcccgcttca gataaatctt ggcgccaccc
     1801 aattttttgg tcagccgatc ggcacgatac agactcgtgg gccgtcctac atattgtttg
     1861 aggtagtaag cgaggtctgt ttgaaatcgg cggtcccgtc tcgcttcggc atattcttct
     1921 tccaactcca agagcgccgg catgagggtt tcgggcacat accggccacc ataggacccg
     1981 aatcggccat ggctgtctgg aagcatgggc atctgatcaa ctcctcctca acggatcgcc
     2041 gggagtatag ccatggcgat cagacggaca caagtctggc cgcttcaata aacgccttaa
     2101 gcttatggtg atctttctta ccagggctca attcaacccc gctgctgaca tcgaccccgt
     2161 aggggcgcac tatctggatc gctccagcca cgttttgggg gttcaaaccg ccagccaaaa
     2221 tgaccggggt agaccgtgcc gcttcctgag ctaacgtcca atcgaccgtc tgtcccgtgc
     2281 ctccatatgt ctgatctgaa aaggcatcga tgagaacccc tcgcacgttc gctcgtccct
     2341 ggaattcagc cagggcgaga aaggtaccac ggtccttcag ccgaagggcc ttcagagccg
     2401 gacggccgag gttctgacaa tacagagctg attcatcccc atgcaattgg gccaagacaa
     2461 agccgcattc atccatgagt gttcgaacct tctcagcctc ttcattgacg aacacaccga
     2521 ccgccaatac aaaaggcgga aggccggcta tgatggatct cgctactgcc ggttccaccc
     2581 aacgtggact ttttctgtac atgacaaagc ccaaggcatc tgccccggcc tttaccgcaa
     2641 cgttcgcgtc tttcacattg gtaataccgc agattttgat cttcatctcg agcgatttag
     2701 gaggcccctt tcctgcctgc agcctgaccg agcaattctc ggactttgtc cgctgtgtgc
     2761 tcggcacgaa tgagcgactc tccgatcaac atggcgtgaa cacctgcttc ggtcagcctg
     2821 gtcacggcat cgcgatcgtg aatgccgctc tcactgatga tcagcttgtc cgaaggaatt
     2881 cttttcgcca accggaaggt cacattaaga tcggtcgtga acgtgttcag atcccgattg
     2941 ttgatcccga tcatcctcgc attgggaatc cactccagga ccgtatccaa ttccttctcg
     3001 tgatgagctt cgaacaggct gtccatcccc agttcagagg ccaatgcatg gaaatccatg
     3061 agttggcgcc gttccaaagc ggctacgatc agcaagaccg catcggcgcc atgagcccgc
     3121 gcttcataaa attgcacgtc gccgaccatg aattccttat tgagcgccgg gatcgggagc
     3181 gcatgcttga tctctttaag ataccgaagg tctccttgga aaaagtcctt atcggtcaag
     3241 acggaaatgg ccgatgcccc atgctcatgg taggtgcgcg caagcccgag ataatcgaac
     3301 tgctccgaaa actcctctcg taaaagccca aggctcggtg a
//
LOCUS       DATMTI010000423         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_313806, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000423 DATMTI010000000
VERSION     DATMTI010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313806"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..455)
                     /locus_tag="VN039_06900"
     CDS             complement(72..455)
                     /locus_tag="VN039_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45734.1"
                     /translation="MGNRTTVTVLIVEDDREMRSLLCDEFFGMGYQLREARNGDEAFL
                     AVLQSVPDLILTDLRMPAGGDDYISRLRTVAPSCPIVVITAFGDATLKAQVVKAGANA
                     YFDKPVRIADLKNCVQELLDHRTDS"
     gene            complement(722..>2030)
                     /locus_tag="VN039_06905"
     CDS             complement(722..>2030)
                     /locus_tag="VN039_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV45735.1"
                     /translation="TISYGDNIHPGDGERVRHEIQAALEQHRLFELTYRILTKSGEVK
                     WVWERGEGIYAQDGTLSYLEGFVTDVTERKRAERLLRQSEERYRRLIAVSPYAILVSR
                     GDRIIFANDQAIKLFGAVKAEEILEQSPMDLFHADDHDLVQERIHELFEGRAQGPMLE
                     ETIETLDGRTVDVEVSGARFVDEEGPAILIMLRDASERKRLQEQLRRTERVAELGTLA
                     SGMAHEIGTPMNVILGRAEYLMDRVTEEPIKRGLRTIITQVERITRVMNQLLSFARRK
                     APQRVPLDLRNVIEDGMEMFQERLARNHIRVEMGMADPCPMVLADADQMSQVLLNLIM
                     NALHAMPDGGTLRVGLEPEQSMVKLTIADTGHGIPPEVIRKIYDPFFTTKEFGKGTGL
                     GLTVVKGIIEEHQGSIAVESEQGKGTRFTILLPRSEELMADGV"
BASE COUNT          436 a          599 c          515 g          480 t
ORIGIN      
        1 aaaaacgagg gtaacagcca aaatcatcgg aggtttcttc tttcccgtca cccgtcataa
       61 gccatatgcg atcagctatc agttctgtgg tcgagcaatt cttgcacaca gttcttgagg
      121 tcggcaatac gaaccggctt gtcgaagtag gcgttcgcac cggctttcac cacttgcgcc
      181 ttcaatgtcg cgtcgccgaa cgccgtgatc acgacaattg ggcagctggg tgccacggtc
      241 cgcaatcggc tgatataatc atcccctccg gcgggcatcc gaaggtccgt caaaatcaga
      301 tccggcacgg actgaagcac cgccaggaat gcttcgtcac cattcctcgc ctcgcgcagc
      361 tgatacccca ttccgaaaaa ttcgtcgcac agcagactgc gcatctctcg gtcatcttcg
      421 acgatcagca cagtcactgt ggttctattt cccaccaacg catcctctgt acataactcc
      481 agcatgttct gaggatggaa gcaaagagga tgccgataag cggtcaacaa agaggcgcgt
      541 gaattcaagg gatcatgata atgatggatt gagacacaca tccagtggcg aaaaacacca
      601 cgattggaag ggagccggtc ggtgcaagat gccacagctg tagaaggtag aggagcagat
      661 ggcggtatgg cttatagcag gagcgggata gggattggtc gctccttatt tccttcttct
      721 gctatacgcc atcggccatc agctcttcgc tcctcggcaa aagaatcgtg aacctggttc
      781 ccttcccttg ctcgctctcc acggcaatgg aaccttgatg ttcttcaatg atccctttca
      841 ccacagtcaa tcccaatccg gttcctttcc cgaactcctt tgtcgtaaag aacggatcgt
      901 agatcttcct gatgacctcc ggtgggattc cgtgaccggt atcggcgatg gtgagtttca
      961 ccatcgattg ctccggctcc aagccgacac gaagcgtacc gccgtccggc atcgcatgaa
     1021 gggcattcat gatgaggttg agcaggactt ggctcatctg atccgcgtcg gccagcacca
     1081 tggggcaagg gtcggccatc cccatttcga ctcggatgtg atttcttgcg agacgctcct
     1141 gaaacatctc catgccgtct tcgatcacgt ttcgtaggtc aagtgggaca cgctgcggag
     1201 ctttccgtcg tgcaaacgag agcagctgat tcatcaccct tgtaatccgc tcaacctgcg
     1261 taatgatcgt ccggaggcct cttttgatag gctcctccgt cacacgatcc atcaaatatt
     1321 cagcccggcc aagaatcacg ttcatcggtg tcccgatttc gtgcgccatg ccggacgcca
     1381 gcgtccccag ttcagcgacc cgttcggtcc tacgcaattg ctcttgaagc cgcttccgct
     1441 cgctggcatc gcggagcatg atcaaaatag ccggtccctc ttcgtcgaca aatcgggcgc
     1501 cgctgacttc gacatctacg gtccttccgt cgagcgtctc gattgtctcc tcgagcatgg
     1561 gcccttgtgc cctgccttca aacagttcat ggattcgctc ttgaacgaga tcatggtcat
     1621 cggcatggaa caggtccatg ggtgactgtt cgaggatctc ctcggccttg actgccccga
     1681 acagcttgat ggcctggtca ttggcaaaga taatgcggtc ccctcgactc actaaaatcg
     1741 catacggcga aacggcgatc aggcgccgat accgttcttc gctttgccgc agcaagcgtt
     1801 cggctcgctt gcgttcggtc acgtcggtca cgaaaccttc caggtaactg agggtgccgt
     1861 cctgcgcata gattccctcg cctctttccc aaacccattt gacctcgccg gattttgtaa
     1921 gaattcggta ggtcaactcg aagaggcgat gttgctccaa cgcggcctga atttcatgtc
     1981 ggacacgctc accgtcgcct ggatggatat tgtcgccata agagatggtt
//
LOCUS       DATMTI010000424         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_567754, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000424 DATMTI010000000
VERSION     DATMTI010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567754"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VN039_06910"
     CDS             complement(<1..424)
                     /locus_tag="VN039_06910"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HWV45736.1"
                     /translation="MATQTGHLRLTYEDYRLIPDDGKRHELIDGEHVLSPSPNTKHQR
                     IARVLLTALTNFLREHPLGEIFIAPYDVVLSDFDVVQPDLIFVATAQAAIVTEANIRG
                     IPTLVVEILSEGNRKLDETIKRQRYEHYGIPEYWIIDPA"
     gene            complement(644..1066)
                     /locus_tag="VN039_06915"
     CDS             complement(644..1066)
                     /locus_tag="VN039_06915"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HWV45737.1"
                     /translation="MLLYRVGLWILFTVLIQAHGPVRAEWVAIDARYQSHPLQTAYID
                     PDTIRREDHLVVVSALVDWKAMQGGRSPTRFYSTKLTKQFDCAERHVRTLAATDFDGH
                     MGMGEVIGGSGYTSEGHWAAIEPGTLNQGLWEAACRKG"
     gene            complement(1176..>2008)
                     /locus_tag="VN039_06920"
     CDS             complement(1176..>2008)
                     /locus_tag="VN039_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWV45738.1"
                     /translation="DDDLLSDPAYTRELCEAMKPLGKKWMSQMSATYVAHHPELLKTL
                     KEAGCSAMFMGIESINQDSLKSVDKQNQAKLYEEMIKRIHDQGIDIHAGFICGLDHED
                     VFDFERTAEWATKMGIAGALWRIMTPYPGTKQFAELKAAGRILTENWTAYTGEHVVYK
                     PAKMTVEQLYWGHKWAKGQFYSYKSIGQRAIQRASQNGFGELLNITGCGLGYRSMFHL
                     AADSAPVNVYRDMNHLPPQSEPIAYRFPYPPKKRFSFVTDRLDQLRSKIQPRPRINKC
                     "
BASE COUNT          429 a          552 c          546 g          481 t
ORIGIN      
        1 gtgccgggtc aatgatccaa tattcgggga tgccatagtg ctcgtaccgt tggcgtttga
       61 tcgtctcgtc tagctttcgg ttgccctcgg acagaatttc gacgaccaag gttggaatcc
      121 cacgaatgtt cgcctcggtc acgattgcag cttgtgccgt ggcgacaaaa atcagatcgg
      181 gctgtacgac atcgaagtcg gacaggacga catcataggg cgcgatgaag atctcgccca
      241 agggatgctc gcgaagaaag ttcgtcaagg cggtcaagag cactcgagct atgcgttgat
      301 gcttggtatt gggcgacggc gataggacat gttctccatc gatcagctca tggcgtttcc
      361 catcgtccgg gataagccgg tagtcttcgt atgtcagtct cagatgaccg gtttgggtcg
      421 ccatatgcac ctcacgccct gttcaactcg cgcgtgtggc caccttaaca aggacaaggc
      481 caaatcgcaa gattcgcaaa gaataagcac caggaaattg caaatgtcgg cttgggagag
      541 atgataatgg ttttcagccc ggtgcagagc ttcttggagt tcaacaaaca aggctggatt
      601 ggtagtttcg ccttcggtag tgggtgtgat gccaatccat tatctacccc ttcctacacg
      661 ccgcttccca caagccttga ttgagtgttc ccggctcaat ggcagcccag tggccttcgc
      721 tcgtgtatcc acttccgcca atcacttcac ccattcccat atgtccatcg aaatcagtgg
      781 ccgccagcgt ccgaacatgt ctttcagcgc aatcgaattg cttggtgagt ttggtggagt
      841 aaaatcgagt gggggaacgg cctccctgca tcgctttcca gtcgaccaag gccgatacca
      901 cgaccaagtg atcctctcgg cggatggtgt cggggtcgat gtacgctgtt tgcaatggat
      961 gtgattgata ccgtgcatca atggccaccc attcggcccg tacaggtccg tgagcctgga
     1021 tcagcacggt gaacaagatc caaaggccta cccgatacaa aagcatggct gtctctccgg
     1081 gaggagctta tcgtaccagc cgcactcttg cgcgtggagc ggacgcacct aggcgattcg
     1141 gggtgtcgat caccatgaag cagcctgcag ttctcctaac atttattgat cctcggtctc
     1201 ggctgtatct tggaacggag ctggtccaat cgatcagtga caaagctgaa ccgtttcttc
     1261 ggcggatagg ggaaccgata ggcgatcggc tccgattgcg gcggcaagtg gttcatgtcg
     1321 cgataaacat tgaccggcgc cgaatctgcc gccaggtgaa acatggagcg gtagcccagc
     1381 ccacagccgg tgatgttgag cagctcaccg aacccatttt gagaggcccg ctggatcgcc
     1441 cgttgtccga tcgacttata cgaataaaat tgccccttgg cccacttgtg tccccagtac
     1501 aactgctcga cggtcatctt ggccggcttg tacacgacat gctcgccggt ataggcggtc
     1561 caattctccg tgaggatccg tccggctgcc ttgagttccg caaactgctt ggtgccggga
     1621 tagggggtca taatgcgcca gagcgctcct gctatgccca tcttcgtcgc ccattcggca
     1681 gtgcgttcga agtcaaagac atcttcatgg tccaagccgc agataaatcc tgcatgaata
     1741 tcgattcctt ggtcgtgaat gcgcttgatc atctcctcat acagcttcgc ctgattttgt
     1801 ttgtcgacgg acttaagcga atcttgattg atcgattcga tacccatgaa catggccgaa
     1861 cagccggctt ctttcaatgt cttgagcaat tccggatggt gcgcgacgta ggtggcgctc
     1921 atctggctca tccacttctt gccgagtggc ttcatggcct cacagagttc ccgcgtatag
     1981 gccgggtcgg acaacaaatc gtcgtccc
//
LOCUS       DATMTI010000425         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_823168, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000425 DATMTI010000000
VERSION     DATMTI010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823168"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /gene="pilM"
                     /locus_tag="VN039_06925"
     CDS             <1..1248
                     /gene="pilM"
                     /locus_tag="VN039_06925"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HWV45739.1"
                     /translation="DLAVGSLVLPTGQTTEGTPRESKGSEVLVTAAPRDKVAEHLRFL
                     AQADVEPSAINVDAMALFSVTQYLQEEGATVPQDLAIIDVGASKTTLCLVQGGRPMVL
                     RTILWGGNHLTHALAVRHVCSFADAERRKRTMAVDQVDGLLEPILKELRITLQAHQGN
                     ERSRLTHCWVCGGGAKLQEIGGYMARQLGLYPVGPRQGFGAEAPRAFSIAFGLAIHPK
                     IIRPRWRFKSTPMELALDLKDVAEAASPDTATTKQDRRLAIAAGLIIAVLAIVDFSIH
                     FMLHDQRVARLKTTLHSRYEQVFGPGAAPGEELDQAQYRLGVLDKALAVTDTADGNVL
                     HTLSAFVKQLPPGTMVKVRELTVDGAAILMEGETTSFDAVERLKQTFASSPQLRDVAV
                     TETRVGAGPNQVVFRITVTVEKP"
     gene            1245..1793
                     /gene="gspM"
                     /locus_tag="VN039_06930"
     CDS             1245..1793
                     /gene="gspM"
                     /locus_tag="VN039_06930"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HWV45740.1"
                     /translation="MTQSFRERWRQMSQRERTLVSAGGIVLGLSLFFVLLVDPLLDTL
                     DRLDRQEVRKQKDLAELAVLGQTYLAKRDRLALTESRMPGADSHFSLLTFMEEAATAA
                     HVRERITGMQPQVQTLAQGYEETAVDLRLEGIQLPDLLALLVAIDQAPYDLHVRHLQI
                     RPKFDNPVNLDATVRVLSYAKS"
     gene            1780..2724
                     /gene="gspK"
                     /locus_tag="VN039_06935"
     CDS             1780..2724
                     /gene="gspK"
                     /locus_tag="VN039_06935"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HWV45741.1"
                     /translation="MPRADERGIALLMALLVLTILTALVLEFDAEARREYRAAAAFRD
                     DYKAGMLTRAAVQAARAVLQQDLFREKMTGQKYDGPTDIWAMPIKNYAIGDGFLTAQI
                     QDETGKFNLNDLASTSGGDIAQKKKILRAKRLFELLRVNPNLVDALIDWIDQDEVPQP
                     AGAESLYYQSLRPPYQSANGPLPGLGDLRLIKGFTPEIIERITPYVTVFPQEGGAMMN
                     VNTADPIVLQTLDPSVTQSVALEIVQGRPYKTKVELDRVGSFQEIGKTLRSDYDIKSD
                     YFSARLAVTVNETTKTAWALLKRDAGKGESTVEYLRLL"
BASE COUNT          621 a          837 c          835 g          543 t
ORIGIN      
        1 gatcttgcgg tcggaagcct ggtgttgccg acgggacaga cgacggaagg gacgccccgc
       61 gagagcaagg gttcggaagt cctggtcacg gccgccccga gagacaaggt cgcggagcat
      121 ctccggttcc tggcccaagc cgatgtggaa ccctcggcga tcaatgtcga cgcgatggcc
      181 ctcttctccg tgacccagta tcttcaagaa gaaggggcga ctgtcccgca agacctggcc
      241 atcatcgacg tcggggcctc gaagacgacg ctttgcctcg tacagggagg acgccccatg
      301 gtcctccgga cgattctgtg gggcggcaac catctgacac atgccctggc cgttcgacac
      361 gtctgtagtt tcgccgatgc agaacgtcga aaacggacga tggccgtgga tcaagtggac
      421 ggtctgttgg agccgatcct gaaagagctt cgcatcacct tgcaggccca tcagggaaac
      481 gaacgcagcc gcttgacgca ctgttgggta tgcggagggg gagccaagct acaagagatc
      541 ggcggctata tggcccgaca attgggactc tatccggtcg gaccgcgaca ggggttcgga
      601 gccgaagccc cacgggcatt ttccattgcg ttcggcttgg cgatccatcc gaagatcatc
      661 cgtccgcgat ggcgattcaa gtcgactccc atggagctcg cgctcgatct caaagacgtt
      721 gccgaggccg cctcgccgga taccgccacg accaaacagg accgccgtct ggcaattgcc
      781 gccggactga tcattgccgt tctggcgatc gtggatttct cgattcactt catgctgcac
      841 gaccagcggg tcgctcggtt aaaaactacg cttcatagcc ggtatgagca agtttttggg
      901 cccggcgccg ctccgggcga agagctcgat caagcccagt accgcctcgg agttctggac
      961 aaggcgctcg cggtcaccga taccgcggac ggcaacgtgt tgcacacgct ctcggcgttc
     1021 gtgaaacaac tcccgcctgg gacgatggtc aaggtgcgcg agttgaccgt ggatggtgcg
     1081 gccatcctca tggagggtga gaccacctcc ttcgacgccg tggaacgact caagcaaacc
     1141 ttcgcatcga gccctcaatt gagggatgtc gcggtgacgg aaaccagagt gggcgcaggt
     1201 cccaatcaag tcgtatttcg aatcaccgtc acggtggaga agccatgacg cagagtttcc
     1261 gagaacgctg gcgccagatg tcgcagcgtg aacgcactct cgtgtcggca ggcgggattg
     1321 tgcttgggct cagcctcttc ttcgtcctcc tcgtcgaccc tttgctggac acactggatc
     1381 gtctggatcg ccaggaggta cggaagcaaa aagacctggc tgaattggcg gtgctcggcc
     1441 aaacctatct cgccaaacgg gaccgcttgg ccttgaccga aagccggatg ccgggagccg
     1501 acagtcattt ctccctactg acgttcatgg aggaagcggc caccgccgcc catgtgcgtg
     1561 aacgcatcac cggcatgcaa ccgcaagtac aaacgctggc gcaaggctac gaggaaacag
     1621 ccgtcgatct ccgattggaa ggcatccaat tgcccgactt attggcatta ctcgtcgcca
     1681 tcgatcaagc cccttatgat cttcacgtcc gccatctgca aatacgaccg aaattcgata
     1741 atccggtcaa tctcgatgca accgttcggg tcttgagcta tgccaagagc tgacgaacga
     1801 ggcatcgcgc tgctcatggc gctgttggtg ctgacgatcc tcaccgccct tgtccttgag
     1861 tttgacgcgg aagcgcgccg ggaatatcga gccgccgccg cctttcggga cgattataag
     1921 gcaggcatgc tgacccgagc cgccgtgcag gctgcgcgag ccgtgctgca gcaagatctc
     1981 tttcgtgaaa agatgacggg gcagaaatac gatggtccga ccgatatctg ggccatgccg
     2041 atcaagaatt atgcgatcgg ggacggattt ctgaccgcgc agatccagga tgagacgggg
     2101 aaattcaact tgaacgacct cgcctccact tcgggaggcg acatcgcgca gaagaagaag
     2161 atcctccgag ccaagcggtt attcgaactg ctcagggtca accccaacct ggtcgatgcc
     2221 ctcatcgatt ggattgatca ggacgaagtg ccccagccgg ccggtgccga gagtctctac
     2281 taccaatcgt tgaggccgcc ctatcagtct gcgaatggtc cgttaccggg attgggagat
     2341 ctccggctca tcaaaggatt cacgccggag atcatcgagc ggatcactcc ctacgtgacg
     2401 gtcttcccgc aggagggagg agcgatgatg aacgtcaaca cggctgatcc catcgtcctt
     2461 cagactctgg atccaagcgt cacccaatcc gtcgcgctgg agatcgtcca aggacgcccc
     2521 tataagacga aagtggaact tgatcgagtc gggagctttc aggagatcgg caagacactg
     2581 agaagcgact acgatatcaa atcagactat ttctccgcgc gcctcgccgt cacggtgaat
     2641 gaaacgacca agaccgcatg ggccttactg aagcgagacg ccggcaaggg ggagagcacg
     2701 gtcgaatacc tgcggttgct ctaatcagct ggtgaaaaac cattcgaaca ctcgcgaatt
     2761 ttgaggctca acgtacgaga caaagtacgc ttcgcctctt cgctcgctgc ggcctcgctg
     2821 gaaggacttt ttgaac
//
LOCUS       DATMTI010000426         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_652884, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000426 DATMTI010000000
VERSION     DATMTI010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652884"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..351)
                     /locus_tag="VN039_06940"
                     /pseudo
     CDS             complement(232..351)
                     /locus_tag="VN039_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(404..670)
                     /locus_tag="VN039_06945"
                     /pseudo
     CDS             complement(404..670)
                     /locus_tag="VN039_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611153.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(803..1869)
                     /locus_tag="VN039_06950"
                     /pseudo
     CDS             complement(803..1869)
                     /locus_tag="VN039_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612267.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1985..2245
                     /locus_tag="VN039_06955"
                     /pseudo
     CDS             1985..2245
                     /locus_tag="VN039_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          451 a          693 c          635 g          480 t
ORIGIN      
        1 ctctatctat agccttacag cgtttgatgc ggcgtgagct gtgatttcga cgcgctgtcg
       61 cctgggacac caagactgcc tgtcttgcct gcgcacgtga gggtaaaacg tggcggtcgg
      121 tgcctcagca tcctgaactt cccccatttg ttacaccacg gccaacgaag ttgactcctg
      181 cgctgtctgg gcaggttgtg atggtggttg atgaactcca ctccataggt gtcagattcc
      241 gatggcactg tgcgtccgct tttcattgta ctctcgtcgc cacgcttcaa tcacgagctg
      301 cgcttcctgc agtgtcagaa accagtgttc gtttaaacag agttggctcc ggttgattcg
      361 acagtgtaga ccattcctta agtggcgcct ggcggattgc tgactacgcg gctgtccgcc
      421 gtgtcgtcag atggtcatag cacacctggt caggcgtccg gccgtcaagc gcctgatgcc
      481 gtcgggtctg gttgtagaac atcagatagc gctccaagct ctgatgcgca accccaaccg
      541 tctcgtaggc atgcaaatac acctactcgt atttgatact tttccagagt cgttccacaa
      601 acacgttatc ccgccagcag ccttttccgt ccatgctgat ctgaatgccg tgatggatca
      661 ggagccccgt gtgtaagctt ccccgtcaag gagacacttc taaaagagaa tttccggctt
      721 ctgacgttgg ccccgttctg agacgattct taaccagagc aggtacttct tcgacggtcg
      781 ctatggcctg acgttggtca atgaactccg tcggggtcag gtgcccgagt gagctgtgcg
      841 ggcggtggtg attgtaatcc atccgccacg cttcaatgat ggcctgtgcc tcggcgagcg
      901 aggcgaactg gtgcatgttc aaacactcat cgcgtaggcg cccgttaaac gactcgatga
      961 aggcattttc caccggtttg cctgggcgaa tgaagtcgag ttggacgccc cgccgatagg
     1021 cccaatcctc caaggcgcgc gattggaatt ccgttccatg atccaccgtg atggagcgtg
     1081 gtcctggccg gtcaccgagc acccgatcaa ggacgcgacc gacgatgtca ccggacatcc
     1141 gaaatcccgc ctctaggacc ggactggagc gactccaatt atccaccacg gtcagaatcc
     1201 gatagggccg tccctcactc aaggtatcat gcacgaaatc catactccac cgttcctggg
     1261 gaccggctgg caccggggct gggcctcgat gcagcgcgat atgtttgcgt cgccgcacgc
     1321 gcatgcgcaa ctggagcccc tccaggcggt accagcgccg cacccgcttg cggttgacgg
     1381 cccagccttc gcggcgaagc aacacccaga tgcgcagata cccaaaccga ggtcgagcat
     1441 gggcgaggtc gcgaatgcgc agccgcaagg ccgtctgatc cttcgcctga ctcttccgat
     1501 accaggaggc ccgaccaaac tgggccaacc gacaggcccg cgcaccactc acctggaacg
     1561 tcccgtgaaa ccactgggcc agttcccggc gccgggcggg cctcagactt tttttcgcag
     1621 cgcctccgac agcatgtgct tatcgagcga gagatccgcc accagccgtt tgagtcgggc
     1681 gttttcctct tccacttgcc gcaaccgccg gagttcgctc acccccagat gggcatactt
     1741 cttcttccag gtatagaagg tggcctcgct gacgccgagc tgtcggcaga ggtcgccact
     1801 cggagtccca ctctcagctt gccggatggc atagacgatc tgctcttcgg aaaatcgtga
     1861 ccgcttcatc gatccactcc tttcgtcgag gaccgccgac gccgccatct tactctcgtt
     1921 tagaactgcc gtcgttttcc ggggggacgt cacacaatga tcccccttta gccaggaggt
     1981 tctcatgcgg cagtcgaagt tcaccgagac gcagattgtg acgatcttga aggaggctga
     2041 tgcgggccgg cccgtcaagg agatctggcg ccaggacggg atcagctccg ccacctatta
     2101 caagtggaag gccaaatacg gggggctgga ggcctccgac gtgaaacggc tgaaggagct
     2161 ggaacacgag aatagtcgcc tgaagcggat gtacgccgag ttgtccttag agaatcaggg
     2221 tctcaaggac gtcatcgcaa aaaagctctg acgcccgcc
//
LOCUS       DATMTI010000427         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_313948, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000427 DATMTI010000000
VERSION     DATMTI010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313948"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..>2778)
                     /locus_tag="VN039_06960"
     CDS             complement(81..>2778)
                     /locus_tag="VN039_06960"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWV45742.1"
                     /translation="EEQKVASMVTGSKICAQRLNAVLKACHTVDGEGALRLDIDPKTN
                     LILREALTSARRDMVPEAYIQRMFSYAQQGFTHFMFHEYDTNWDGKAYQTVSGQNSNN
                     SVRIPNEFFAALETDGDWQLKRRTNGKVCKTVKARGLWDRIAWAAWICADPGTQYDTI
                     INEWHTCPEDGRINASNPCSEYMFLDDTACNLASLNLTKFYTADGQFELEHFRHAVRL
                     WTIALEISVLMASFPSRSIAEKSYQFRTLGLGYANLGTVLMRQGIPYDSPKALAICGA
                     ITAIMTGEAYGTSAEMAAELSPFPGYAKNREHMLRVIRNHRRAAYQVPHAEYEGLTIT
                     PAGIRPEHCPPDMLLAARRAWDHALELGTAYGYRNAQVTVIAPTGTIGLVMDCDTTGI
                     EPDFALVKFKKLAGGGYFKIINQSLPMALANLGYTDQQTQDVIRYCVGAQTLKGAPFI
                     NHDTLRQKGFDDAALGRLESSLGQAFEIQFAFNKFTLGEVFCVEKLGLTEAQLNGANF
                     NMLKTLGFTQEEIAAANDFCCGTMTVEGAPYLKAEDLAVFDCANRCGRIGQRSIAVDA
                     HIRMMAAAQPFISGAISKTINMPADATLEDVKAAYLLAWKSMVKAVALYRDGSKLSQP
                     LSASTDSGKAVETATGVMELAEKVTERVLVRYLAKRRPLPSRRSGYTQKAIVGGHKLY
                     LRTGEYEDGTVGEIFLDMHKEGAAFRSLMNCFAIAISLGLQHGVPLEEFVEAFVFTRF
                     EPNGPVKLNDRIKMSTSIIDYIFRELAVTYLDRYDLAQVNEEDLRMDSVKKDHMDPEC
                     FDEEADLDALAKSSVLTEHLPIRRNGGKGNGHGNGHSVARQVEIMRETLTLTAVQNAK
                     NAKEAKVKGYEGDPCQECKQFTMVRNGTCLKCVTCGATSGCS"
BASE COUNT          597 a          815 c          704 g          662 t
ORIGIN      
        1 ccgatcgaat tcgcagaaac cgccgcttct tacaccggag acacgatgac ggccgtttgc
       61 cctgcatcct caatctcctc ttatgaacat ccactcgttg cgccgcatgt cacacatttg
      121 agacaggtac cgttacgaac catggtgaat tgcttacatt cttgacaggg gtcgccttca
      181 tagcccttca ccttggcctc tttagcattt ttagcgtttt ggaccgccgt cagcgtgagc
      241 gtttcacgca tgatctccac ttgtcgggcg acactgtgtc cgtttccgtg gccatttcct
      301 tttccgccgt ttcggcgaat gggaagatgc tccgtaagca cggaagattt agccagtgcg
      361 tccagatccg cttcctcatc gaagcattcg ggatccatat gatccttctt caccgaatcc
      421 atgcgcaggt cttcttcgtt cacctgcgcg aggtcatacc gatcgagata cgtaacggcc
      481 aattcacgga agatgtaatc gatgatcgaa gttgacattt tgattcgatc gttcaatttg
      541 acgggaccgt tcggctcaaa ccgggtaaag acaaacgcct caacgaattc ttccagcggc
      601 acgccgtgct gaagtccgag agagatggcg atcgcaaaac aattcatgag gcttctgaac
      661 gccgccccct ccttgtgcat atccaaaaag atttccccga ccgtcccatc ctcatattca
      721 ccggtgcgca ggtagagctt atgtcccccg acaattgcct tttgagtata tccgcttcgt
      781 cggctcggta aggggcgccg cttggcgaga taccgaacaa gcacccgttc ggtgactttc
      841 tccgccagct ccatcacgcc ggtggccgtc tccacggcct ttccactgtc tgtcgaagca
      901 ctcaacggct gactcagctt ggatccgtca cgatacagtg cgaccgcttt gaccatgctc
      961 ttccaggcaa gcaaataagc cgccttgacg tcttcaagcg tggcatcggc cggcatatta
     1021 atggtcttgc tgatcgcgcc gctgatgaac ggctgtgccg cggccatcat gcggatatgc
     1081 gcatcgacgg cgatggagcg ttgtccgatt cgaccgcacc gattggcaca gtcgaatacc
     1141 gcgaggtctt cggccttcaa atacggcgcg ccttccaccg tcatcgtccc gcaacagaaa
     1201 tcgtttgccg cggcgatctc ttcttgcgta aaaccgagcg tcttcagcat gttgaagttc
     1261 gctccattca gttgagcctc ggtcaaacca agtttttcga cgcagaacac ctctcccaat
     1321 gtaaacttgt tgaaggcgaa ttgaatttcg aacgcttgcc ccaaactgct ctccaaacga
     1381 cccagggccg catcgtcgaa tcccttttga cgcagcgtgt cgtgattgat gaacggtgcg
     1441 cccttaagcg tctgagcacc gacacagtac cggatgacat cctgggtttg ctggtccgta
     1501 tagccgaggt tggccagcgc catcggcaag ctttgattga tgatcttgaa atatcctcca
     1561 ccagccagtt tcttaaactt caccagagca aagtcgggtt caatccccgt agtatcacag
     1621 tccatgacca acccaattgt gccggtcggc gcgatgacgg tcacttgtgc attgcggtac
     1681 ccataggctg ttcctaattc aagcgcatga tcccacgctc gtctggcagc aagaagcatg
     1741 tcgggaggac aatgctcggg gcggattccc gccggagtaa tcgtcagacc ttcatattcc
     1801 gcatgaggta cctggtaagc tgcccgacgg tgattacgga tgacacgcag catgtgctct
     1861 cgattttttg cataccccgg gaagggcgac aattcggcag ccatttcagc cgacgtcccg
     1921 tatgcttccc cggtcatgat ggccgtgatg gctccacaaa tcgccagggc cttgggtgaa
     1981 tcataaggaa ttccttgtcg catgagcacg gtccccaaat ttgcataacc caacccgagc
     2041 gtccggaact gatagctctt ttcagcgatg gaacggctcg ggaaggaagc catcaacacg
     2101 ctgatctcta acgcgatcgt ccacagtcga acggcatggc ggaaatgttc cagttcgaac
     2161 tgcccgtcgg cggtgtaaaa cttcgtgaga ttgagagagg cgagattaca ggccgtatca
     2221 tccaaaaaca tgtattcgga gcaggggttc gacgcgttga tgcggccgtc ttccgggcac
     2281 gtatgccact cgttgatgat ggtatcgtac tgagtccccg gatcggcaca aatccaggcc
     2341 gcccaagcga ttcggtccca cagtccccgc gccttgacgg tcttacaaac cttgccgttc
     2401 gttcggcgtt tgagctgcca atcgccgtct gtttcaagcg cggcaaaaaa ctcattggga
     2461 atcctgacgc tgttgttcga attttggccg gagacggtct ggtaggcctt accgtcccaa
     2521 ttcgtgtcat actcgtgaaa cataaagtgc gtgaagcctt gctgcgcata ggaaaacatt
     2581 cgttggatat acgcctcggg caccatatcc cgacgggccg atgtcagggc ctctcgcaag
     2641 atcagattgg tcttcggatc gatgtccagc ctgagagccc cctcgccatc gaccgtatga
     2701 caagctttca gcacggcgtt gaggcgctgc gcacagatct ttgaacccgt caccatcgac
     2761 gcgacttttt gttcttcg
//
LOCUS       DATMTI010000428         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_229903, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000428 DATMTI010000000
VERSION     DATMTI010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229903"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..449
                     /locus_tag="VN039_06965"
     CDS             252..449
                     /locus_tag="VN039_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45743.1"
                     /translation="MRRQFRFRVSGHMRVGEDLVAFTRVVRASSQERAIQAVKFFVPR
                     EPRYEKFRVVFLDDTIKAQLS"
     gene            464..709
                     /locus_tag="VN039_06970"
     CDS             464..709
                     /locus_tag="VN039_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45744.1"
                     /translation="MSVNTTFVLSDESNPKGTKLDVSSNIFENNGKRNACFTVARGNG
                     SVTVITDNPQDLWTIAAELDSLARDLYERNIDPTNGS"
     gene            720..1175
                     /locus_tag="VN039_06975"
     CDS             720..1175
                     /locus_tag="VN039_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45745.1"
                     /translation="MAHLPRICGCTTEELPPPNATAPMAAVSVACEHGQGDWTAFSDI
                     NPGFQSLPLATIVAGTVQRMTVQMETNGKDWFPHTEFYLFHYAEVEPCTNFCAALHVR
                     HSGHLDMVWDPCPKRPLTFHSRMDAGEWALREFTDAKNGSVDFTFVVID"
     gene            1301..1648
                     /locus_tag="VN039_06980"
     CDS             1301..1648
                     /locus_tag="VN039_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45746.1"
                     /translation="MAFEILDDIDFEAKPREYKGGAKGPRPRTEEQKPWDEAFDNAWK
                     SEKKVLAAQVTPDMAEDAKKHVLSSARYYGLAVTEGKAIPGKVEGTVILRWKIREPKK
                     RVASRTDVNTSDE"
BASE COUNT          411 a          513 c          487 g          347 t
ORIGIN      
        1 caaatggcaa gtctccaacg acaacaagtt cgggaagaaa gcttttaccg ccatcgctgt
       61 gcgacacgca gactcgcgtc ttcactctgt tgaagttcat cacgacggac aagtcgacgt
      121 tatcaaaccg caatctttca aggatgttcc gctgattgac gacttgctac ttccatgaaa
      181 gtgttgtcct actgcttgag agcagagcac cttgataact caacagagtg ttaccggaca
      241 agggagggaa catgcgtcgc cagtttcgat tccgcgtttc tggtcacatg cgagtaggcg
      301 aagacctcgt agccttcacg cgtgtcgttc gcgcatcaag tcaagagcgt gccattcagg
      361 cagtaaagtt ctttgttccg cgcgaacctc ggtatgagaa gttccgcgtc gtctttctcg
      421 acgacacgat caaggctcag ttgagctgaa agggtgggga gtcatgagcg tcaacaccac
      481 gttcgtcctg tcggatgaaa gcaatcccaa gggaaccaag ctcgacgtca gcagtaacat
      541 cttcgagaac aacggtaagc gaaacgcatg cttcaccgtt gcgcgcggta acggcagtgt
      601 taccgtgatc accgacaacc ctcaggactt gtggacgatt gccgcagagc ttgactccct
      661 cgcccgtgac ctgtacgaac gcaacatcga cccgacaaac ggaagctgag agaaaagcca
      721 tggcgcacct cccccgcata tgcggctgca ccacagaaga actgcccccg cccaacgcta
      781 ccgcccccat ggctgcggtt tccgtcgcct gtgaacacgg gcaaggtgac tggactgcat
      841 tctcggacat caatcctgga ttccagtcac tcccactcgc taccatcgtc gccggaaccg
      901 tccagagaat gacggtgcag atggagacca acggtaagga ctggttccct cacactgagt
      961 tctatctctt ccactacgcg gaagtggagc cgtgcaccaa cttctgcgca gctctccacg
     1021 tccgtcacag tggtcacctg gacatggtgt gggacccttg ccccaagagg ccgttgacct
     1081 tccattcccg catggacgct ggtgagtggg cattgcgtga attcacggac gccaagaacg
     1141 ggtctgtcga cttcacgttc gtggtgatag actgacctag accctttcgg ggtcgcacag
     1201 gaaaggttgc cgacagtgac agggatcact ccaagggttg aagtgtcgcc cacgagtgag
     1261 ggagtaatcc ctgtcactgt cccaagaaag ggttgaaaca atggcatttg aaattctcga
     1321 cgacatcgat ttcgaggcaa agcctcgcga gtacaagggt ggcgctaagg gtcctcgccc
     1381 gcgcactgag gagcagaagc cgtgggatga ggctttcgac aacgcgtgga agagcgagaa
     1441 gaaggtcctt gccgcacagg tgaccccgga catggccgaa gatgccaaga agcacgtcct
     1501 gtccagtgcc cgctactacg gccttgccgt gactgagggc aaggccattc ccggcaaggt
     1561 cgaaggcacc gtcattctcc gctggaagat ccgtgagccc aagaagcgtg tcgcttcccg
     1621 cacggacgtc aatacctctg acgagtagtt ccgcccccac cccatgatgc gcgggaacgg
     1681 tgtgcgtcgc ctagtacccg tcatgtagga accactcgcc agagtgcgcg acaagacctg
     1741 atgtcgcgct gaatgggc
//
LOCUS       DATMTI010000429         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_823303, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000429 DATMTI010000000
VERSION     DATMTI010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823303"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="tilS"
                     /locus_tag="VN039_06985"
     CDS             complement(<1..724)
                     /gene="tilS"
                     /locus_tag="VN039_06985"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWV45747.1"
                     /translation="MKQSEGDESLQPTLNRPIDKMAWPPLVHRVVRTVRLRSLFQRGQ
                     HIVVAISGGPDSVALLSILHLLRSSWKLSLSAVHCNYGLRGAESDEDQKFVEAFCQVL
                     GMPLHVRRVEFHTGGPKTSLQAEARDLRYRLMQEIAEKCGADRIAVGHTADDQAETIL
                     LWMLRGAGLTGLSGMPAFRDNKIIRHLYDIKRQEVLAYLRTAGLSFREYSSNFQMHYL
                     RNRVRREVIPILNRLVPSCVDAL"
     gene            complement(702..1658)
                     /locus_tag="VN039_06990"
     CDS             complement(702..1658)
                     /locus_tag="VN039_06990"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWV45748.1"
                     /translation="MKVTRGYSDEVVRPYPVGTIGNFDGHHLGHHALLKRVVERARRV
                     QGTAVVLTFDPHPVKILAPHVDLRFLTSWDEKRARFEQAGIDEVISLEFTQVFASLSP
                     EMFAEQVLSKGLGLKEIFVGQHFAFGHKRVGQIDDLTALGERFGFIVHPTPPVMINGG
                     VVSSTSIRQLIVAGHVDQAAVLLGRHYALSGVVSPGEGRGRTLGCPTANLRLPPDRVA
                     PADGIYASVVAIDQERHDSVAYIGTRPTFATFDGAGRGLEVSILDGIHELYGRTLTVE
                     FIDRIRGDAQFDSAEALRRQITADVDSARGILRRYHETIGGR"
     gene            complement(1665..2636)
                     /gene="hemB"
                     /locus_tag="VN039_06995"
     CDS             complement(1665..2636)
                     /gene="hemB"
                     /locus_tag="VN039_06995"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWV45749.1"
                     /translation="MGFPIQRLRRLRQHESLRRMVRETALSPSDFIYPLFVIEGQDRR
                     EKIASMPGQFRLSVDLLVKEAGEIQALGIPAIILFGIPAHKDEHGSSGWDPNGIVQRA
                     IRAVKQQVPGLLVITDVCIDEYTSHGHCGIVKDGKVLNDETLECLTVMARTHAEAGAD
                     MVAPSDMMDGRVAAIRRELDRSGFSDLPILAYAAKFSSCFYAPFRDAAFSSPQFGDRQ
                     SYQMDPANAREALREIDLDIDEGADIVMVKPALPYLDIIAMARARTLLPLAAYQVSGE
                     YSMIKAAARAGWLDESRAMMESLLAIKRAGADLILSYFAKDAVKLLH"
BASE COUNT          634 a          806 c          693 g          564 t
ORIGIN      
        1 agagtgcatc gacgcaggaa gggaccagcc gattcagaat cggaataacc tctctcctta
       61 ctcgattccg caagtaatgc atctggaaat tgctggagta ctcccgaaac gacaatcctg
      121 cagtgcggag atacgccagg acttcttgtc gcttgatgtc gtacaaatgc cgaataatct
      181 tgttatcgcg gaacgccggc ataccggaaa gaccggtcag tcccgcacca cggagcatcc
      241 acagcaagat cgtctctgct tgatcgtccg ccgtatgacc tacggcgata cgatccgccc
      301 cgcacttctc ggcaatctcc tgcatcagcc gatagcggag gtcacgggcc tcagcctgca
      361 gcgaggtctt cggcccaccg gtgtgaaatt caacccgtcg cacatggaga ggcatcccaa
      421 gcacttggca aaaggcttcc acaaattttt ggtcttcgtc ggactctgcc cctcgcagtc
      481 catagttgca atgaacggct gataggctca gcttccaact cgaccggagg agatgcagga
      541 tcgataacag tgcgacggaa tccgggccgc ccgagatggc gaccactatg tgctgtcctc
      601 gttgaaagag gcttctgagt cgcaccgttc gaacgacacg atgcaccagc gggggccagg
      661 ccattttatc gataggtctg tttagggtcg gttgcaaaga ctcatcgccc tccgattgtt
      721 tcatgatatc gacggagaat gcctctcgca gaatccacgt cggccgtgat ttgccgcctt
      781 agagcttctg cgctatcaaa ctgcgcgtcg cctcgtatac gatcgataaa ttcaaccgtg
      841 agcgttcgtc catacaactc gtgtatccca tccaggatgg atacttccaa tccccgtccc
      901 gccccatcga aggtcgcaaa ggtcggtctc gtaccgatat aggcgaccga atcatgtcgc
      961 tcctgatcga tggccacgac cgaggcataa attccatcgg caggagccac gcggtctgga
     1021 ggcagtcgaa gatttgcggt aggacatcct aatgttcgcc ccctcccctc gccaggagat
     1081 acgaccccac tcagagcata gtgtcggccg agcaagacag ccgcttgatc gacatgccct
     1141 gcgacaatga gttgcctgat actcgtcgag ctgactaccc ctccattgat catcaccgga
     1201 ggagtcggat gaacgatgaa accgaatcgt tcgccgagcg cggtcaaatc gtcgatctgt
     1261 ccgactcgct tgtgcccaaa ggcaaaatgt tgccccacaa aaatctcttt gagtccaagc
     1321 cctttagaca gaacctgctc agcgaacatc tctggggaaa gtgacgcaaa tacttgagtg
     1381 aactccaggg aaatcacttc atcgattccg gcttgttcga aacgggcacg tttctcgtcc
     1441 cagctcgtca ggaaccgcag atcaacgtga ggggcaagaa ttttcaccgg gtgagggtcg
     1501 aatgtcagga cgacggccgt tccctggacg cggcgtgccc tctccaccac ccgtttcaaa
     1561 agcgcatgat gaccgagatg atggccatcg aaattcccga tggtgcccac cggatatggc
     1621 cgtacgacct cgtccgaata tccgcgggtc actttcatcg tcgatcaatg aagcagctta
     1681 acggcgtctt tcgcgaaata cgacaggatc agatcggctc ccgcccgctt gatcgccaac
     1741 agcgattcca tcatggcacg tgattcatcg agccaccctg ctctcgctgc cgccttaatc
     1801 atactgtact cgccgctcac ctgatacgcc gccagcggga gaagcgtccg agcacgggcc
     1861 atcgcgataa tgtccaaata aggcagcgcc ggcttgacca tcacaatatc ggcaccttcg
     1921 tcgatatcga gatcgatctc gcgcagcgct tcacgcgcat tagccggatc catctgatag
     1981 gactgtcggt caccgaattg aggactggaa aaggccgcgt cgcgaaaagg tgcataaaaa
     2041 caggaggaga atttggcggc atacgccagg atcggcagat cggagaaccc ggagcgatcc
     2101 agttcacgtc tgattgcagc gacacgacca tccatcatat ccgacggtgc gaccatatcg
     2161 gctccggcct cggcatgagt acgtgccatt accgtgaggc attcgagcgt ttcatcattg
     2221 agaacttttc catccttgac gatcccgcaa tggccgtggc tggtatactc gtcgatacag
     2281 acatccgtga tcacaagtaa tccgggcact tgttgtttga cagctctgat cgcccgctgc
     2341 acaatgccat tgggatccca ccccgagcta ccatgctcgt ctttgtgcgc cggtatccca
     2401 aagagaatga tggctggaat acctaaggcc tgaatctctc ccgcctcttt gaccagcaga
     2461 tcgacggaga gccgaaattg gcccggcatc gaagcaatct tttcacggcg atcttgtccc
     2521 tcgatcacaa acagcggata gataaaatcc gacggggaca gcgccgtttc ccgtaccatc
     2581 cgacgcaacg attcatgttg tctcagtcgt cggagccgct ggatcggaaa ccccatcgcc
     2641 cgcctacttt cgtggaaggt tcgtcaagaa cacaacgagg tcgcgaacgg aaataac
//
LOCUS       DATMTI010000430         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_398651, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000430 DATMTI010000000
VERSION     DATMTI010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398651"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1525
                     /gene="dnaG"
                     /locus_tag="VN039_07000"
     CDS             <1..>1525
                     /gene="dnaG"
                     /locus_tag="VN039_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWV45750.1"
                     /translation="NLVGLCPFHQEKSPSFSVSPSKQMFYCFGCKAGGDVFAFLTKIT
                     GATFPEVLKELGDKVGIMVEESLAERVHRGQTHRLEEINRVAMAWFQANLRDPQIGAT
                     AREYLNGRGIQQSTVDSFQIGVSSPEWDGLLKFLSRKGFSHSEIMTAGLGSPRPNGNG
                     YYDKFHGRLMFTITDLRRRVVGFGGRVLGDRMPKYLNSPDTLLFKKGQTLFAFDQARE
                     AIVRTKTVIIVEGYFDAIALHQAGLTHTVATLGTALTAEHVQALRRFADQVVLLFDPD
                     AAGVRAALRGLDLFVNSGLGVKVVTLPVDEDPDTFVRKKGAVTFAQLEAAAPSLLDYA
                     LNHTVKQADDGSLESRIRSVDEVLRILQKSEHPIERQERIRIVSERLGISETLLIERY
                     PALLAQPKRGGEGPRAQATQGISFNALFKGIPEERDLLLLLLHGKLSPADVSRLRPES
                     FTVAPCRKLVEIALAHVDREGCISLRSVLDESVDDPDCGALATELSMREDHFDDVQAH
                     "
BASE COUNT          325 a          398 c          464 g          338 t
ORIGIN      
        1 gaaccttgtg gggttgtgcc catttcatca ggagaagagc ccctcattct cggtcagtcc
       61 ttccaaacaa atgttttact gcttcggctg taaggccgga ggcgatgtct tcgcctttct
      121 gaccaaaatt acgggggcga cctttcctga agtgttgaag gaacttggag acaaggtggg
      181 cattatggtg gaggagtcgc ttgcggaacg agtgcaccgt gggcagaccc atcgtcttga
      241 agaaatcaac cgcgtcgcga tggcctggtt ccaagccaat ctgcgggatc cgcaaatcgg
      301 tgctacggct cgtgagtatc tgaacggacg aggtatccaa caatcgacag tggattcgtt
      361 ccaaattgga gtgtcgtccc cagagtggga tgggttgttg aaattccttt ctcgaaaagg
      421 attctctcac agcgagatta tgacggctgg gctcggaagc ccaagaccaa acggtaatgg
      481 atactacgac aagttccatg ggcggctcat gttcacgatc accgatctgc gcaggcgcgt
      541 ggtgggattc ggcggacgtg tgttgggcga ccgcatgcct aagtatctca attcaccaga
      601 tacattgttg tttaaaaagg gccagaccct tttcgccttc gatcaggcac gcgaggcgat
      661 cgtgcggacc aagacggtga tcatcgtcga aggctatttt gacgcgatcg cgctccacca
      721 ggcggggctt acccataccg tcgccacgct ggggacggcc ctgacggctg aacatgttca
      781 ggccttgcgg aggtttgccg accaggtcgt gttgctcttt gaccctgatg cggccggtgt
      841 gcgcgcggca ttgagaggat tggatctttt cgtcaacagc ggattgggcg tcaaagtcgt
      901 aactcttccg gttgacgagg atcctgatac gtttgtgcgg aagaaaggtg ccgtgacgtt
      961 tgcccagctg gaagcggctg ctccgagtct cttggactac gcactgaatc acacggtcaa
     1021 gcaggccgac gacggctcac ttgaaagtcg catccgaagc gtcgatgagg tgctgcgaat
     1081 tctgcagaaa agcgagcatc cgatcgagcg gcaggagcgc atcagaatcg tgtcggaacg
     1141 gctgggaatc agcgagaccc tgctgatcga acgctatccc gcgcttctgg ctcagccgaa
     1201 acgaggcggc gaagggccgc gcgcgcaggc cacacaggga atctcgttca atgccctttt
     1261 caaagggatc ccggaagagc gggatctcct cttactgctg ctgcacggaa aattgtcgcc
     1321 tgccgacgtc agccgcctgc gaccggaatc ctttaccgtc gcgccatgcc gcaagcttgt
     1381 cgagattgca ctcgcccatg tcgatcgaga ggggtgtatc agtctcagat cagttttgga
     1441 tgaatcggtg gatgatcccg actgtggtgc tctggcgacg gaattgtcga tgcgtgagga
     1501 tcatttcgac gatgtgcaag cgcac
//
LOCUS       DATMTI010000431         1889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_483610, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000431 DATMTI010000000
VERSION     DATMTI010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483610"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="VN039_07005"
     CDS             <1..1445
                     /locus_tag="VN039_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HWV45751.1"
                     /translation="SDRPAGEYGRYAAVRRDREHPGRVSAGRPAGDRPAEPEREFTVI
                     VKGGSRKSGAFFATHLMKTEENERVSIAEMKGLYAEDVPEAFRELKALAAGTRAENYF
                     YHASINPREDELLTSEQWEFAVDRLEHNLGLDGHARFQVEHEKEGRTHRHIVWSRVDP
                     DTMTITSDSFTYLAHDHTRAELEQAFHHEPTPPTPQPSQRRSREIHEWENFRAQESGI
                     DAKEVKAEVTALWQHSDSGSAFVAALEDSGYTLCQGDRGYCIIDSYGDTHSLVRRLDG
                     VKTAELREYLSGMPLASLPTVAEASAAVRARNEAEEEGSTGGATLLVTEEQAQREPHV
                     AELGDTNPKLRVLNQLAEEHRAQEAPDPRAAWFAENLPDTYPVAANARAYAELVEADM
                     RANDCELPFTQGRTWEMWADNAMQYFRERAESLSEQVHDLPPELQERLEHLKDTVTDV
                     KDRLSTFWQDFVKEGREREPPEIQEPELER"
     gene            1450..>1889
                     /locus_tag="VN039_07010"
     CDS             1450..>1889
                     /locus_tag="VN039_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45752.1"
                     /translation="MSQDEDEDDAVLQLWGLGQAEPPKLVVNNSTPPYVALDTQDRLL
                     GFTLHCHSVKLAYTFFYPHLLSITIHEPEGDFVVVTTSTSIIRIYGHNLQPIVSALNS
                     HTCKALWEFSKELYLPPPGDDGKPFIEKIDVALIKGGESMKQRV"
BASE COUNT          449 a          532 c          567 g          341 t
ORIGIN      
        1 aatcagaccg cccggcgggt gaatatgggc gatacgccgc tgtccggaga gatcgcgagc
       61 accctggccg cgtgtcggca ggtcgcccgg caggtgaccg acctgctgaa ccggaacggg
      121 agttcacggt gatcgtcaaa ggcgggagcc ggaaatccgg ggcgtttttc gccacccacc
      181 tgatgaaaac cgaggagaat gagcgggtga gcatcgccga gatgaagggg ctgtacgctg
      241 aggatgtgcc ggaagccttc cgtgaactca aagccctcgc cgccggcaca cgcgccgaga
      301 actattttta ccacgccagc atcaaccctc gagaagatga acttctgact tcggagcaat
      361 gggaatttgc cgttgaccgc ttggagcata atttagggtt ggacggccat gcccgcttcc
      421 aagtcgaaca tgagaaggaa gggagaaccc accgccatat tgtctggagc cgggtcgatc
      481 ctgacaccat gacaatcacc tctgacagct tcacctatct ggcgcacgac cacacccgcg
      541 ccgagctgga gcaggcattt catcacgagc caaccccgcc gactccgcag ccgagccagc
      601 ggaggagccg tgagattcac gagtgggaaa acttccgggc gcaggaaagc ggtatcgatg
      661 cgaaggaggt gaaagccgaa gtgacggcac tgtggcaaca cagcgattct ggttcggcct
      721 tcgtcgccgc actggaagat tccggttata ccctctgtca gggcgaccga gggtactgca
      781 tcattgacag ttacggcgat acccattctt tagtaagacg gctggacggc gtgaaaactg
      841 ccgaactccg tgagtatctt tccggcatgc cgctggcgag tctcccgacc gtggccgaag
      901 ccagcgccgc cgtgcgggca aggaatgaag cggaggaaga ggggagcacg ggcggagcta
      961 cactcttagt cacagaagaa caggcgcagc gtgagccgca cgttgcggaa ttgggtgata
     1021 ccaatcccaa actgcgtgta ctcaatcaat tggccgaaga acaccgagcg caggaagcac
     1081 ctgacccccg tgccgcctgg tttgcggaga atctgccgga tacgtatccc gtcgccgcta
     1141 atgcccgcgc ctatgccgaa ctagtggaag ccgacatgcg agcgaatgat tgcgaactac
     1201 cgtttaccca aggccggaca tgggagatgt gggcagacaa cgcgatgcag tattttcgag
     1261 aacgggcgga gagtctttcc gagcaagtgc acgatctccc accggagctc caggagcggc
     1321 ttgagcatct gaaagacacc gtgactgacg tgaaggacag gctcagcacc ttctggcagg
     1381 acttcgtaaa agagggacgt gagcgcgagc cgcctgagat tcaagaaccc gaactggaga
     1441 ggtagtgcga tgagccagga cgaagatgag gatgatgctg tcctgcaatt gtgggggctc
     1501 ggccaagctg aaccgccaaa actggtggtg aacaattcca caccgcccta cgtggctttg
     1561 gacactcagg atcggttact cggctttacg ctgcactgtc actcagtcaa actcgcctat
     1621 accttcttct atccgcatct tctcagcatc accatccatg agccggaggg tgattttgtg
     1681 gtggtgacga cttccaccag tatcatccgc atttacggcc ataacctgca gcccatcgtc
     1741 agcgcgctca actcccacac ttgtaaagcc ctgtgggaat tttccaaaga gctgtacctg
     1801 ccgccgccgg gcgatgacgg caagcccttt atcgaaaaaa ttgacgtggc gctgatcaag
     1861 ggcggagaga gtatgaagca gagagtgaa
//
LOCUS       DATMTI010000432         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_101029, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000432 DATMTI010000000
VERSION     DATMTI010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101029"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..298
                     /locus_tag="VN039_07015"
     CDS             89..298
                     /locus_tag="VN039_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45753.1"
                     /translation="MSPDLTSKITLWRAKAVEGTLTLEEMKEAIQLLRGERVSAAQAS
                     DKARRTKAIKAIPTADDLLKELGEL"
     gene            301..501
                     /locus_tag="VN039_07020"
     CDS             301..501
                     /locus_tag="VN039_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45754.1"
                     /translation="MATVDKKFADRIIAGEWPEDEAQRIVKYTNAWGGESYGVTFPGD
                     PPDKYLIESEYIQNPTIYWEAP"
     gene            498..1412
                     /locus_tag="VN039_07025"
     CDS             498..1412
                     /locus_tag="VN039_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWV45755.1"
                     /translation="MKVKPPFPSVLDASLISTFRACPQKAFLEYVQHWKPRSPSVHLH
                     AGAAYASALEATRRAFYLEGKSSSEAAEIGMATLLREYGDFPCPEDSAKSASRMAGAL
                     EFYLHSYPLESDAAVPVTLPDGSKGIEVGFAEPLPINHPETGDPLIYAGRLDMMVNYA
                     GAIYGLDDKTSSSLGASWSKQWEMRSQFTGYCWGAARLGFPLSGFLVRGVSILKTKYD
                     TQQALTYRPQWMIDRWYEQLLRDIHDMLECWESGIWDWNLDASCEAYGGCPFKKVCLA
                     AEPDAWLSTGFERRSWDPITRKESLLES"
     gene            1409..>1719
                     /locus_tag="VN039_07030"
     CDS             1409..>1719
                     /locus_tag="VN039_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45756.1"
                     /translation="MKQSFFVRGHFLGSREIPTYYKSYDGQLKITNSYVLFCPTCAEI
                     WGRLMHEHWNAHCQILPRYCREHARFPDDGFFSLRGYEDKPACLPGMVLESDWPLEAI
                     R"
BASE COUNT          432 a          512 c          394 g          381 t
ORIGIN      
        1 tcgcagatgc cttagttgaa actgccaagc tagtcggcaa gacaatgacg accgaaatcc
       61 tcgaacaaac caaagccctt ccaaaatcat gagccccgac ctcacctcca aaattaccct
      121 ttggcgtgcg aaggctgtcg agggaactct caccctggaa gaaatgaagg aagcaattca
      181 gcttcttcgc ggggaacgag tctccgctgc ccaagcatcc gacaaggcgc gcagaaccaa
      241 agcgatcaaa gcaatcccca cggcggatga tcttctcaaa gaacttggag aactttaatc
      301 atggctactg tagataaaaa attcgcagat cgtatcatag cgggcgaatg gccggaagac
      361 gaagcccaac gcatcgtgaa atatacgaac gcttggggcg gagaatccta tggtgtaacc
      421 ttccccggcg accctcctga taaatacctc atcgaatccg agtacatcca aaaccctacc
      481 atatattggg aggctccatg aaagtaaagc cacccttccc ttctgttcta gatgcatccc
      541 tcatctcgac cttccgtgcc tgcccacaaa aggccttcct cgaatacgtc caacattgga
      601 agccgagaag cccttccgtc caccttcatg ccggggcggc ctatgcttct gccctcgaag
      661 ctacgcgtcg agccttctac ctagaaggca aatcatcctc cgaagcagcc gaaattggca
      721 tggccaccct tctccgcgaa tacggagact tcccatgccc cgaggactcc gcgaagtccg
      781 cttcccgaat ggctggagcg ctggagttct atctccactc ctacccactc gaatctgacg
      841 cggcagtccc cgtgaccctc cctgacggca gcaagggaat cgaagtagga ttcgcagaac
      901 cacttcctat caatcatccc gaaactggcg accctctcat ctatgctgga cgcctagata
      961 tgatggtgaa ttatgcagga gctatctacg gtcttgacga taagacaagt tcctcccttg
     1021 gcgcctcgtg gtcaaagcag tgggagatgc ggagtcaatt caccgggtat tgctggggcg
     1081 ctgcccgtct cggattcccc ctatccggtt tcctcgtccg cggtgtttcc atcctcaaga
     1141 caaaatacga tacccaacaa gccttgacat atcgtcctca gtggatgatt gacaggtggt
     1201 acgagcaact cctccgcgac attcacgaca tgctggaatg ctgggaatct ggcatctggg
     1261 attggaacct tgatgcctca tgtgaagcct atggaggctg cccgttcaaa aaggtatgtc
     1321 ttgcggctga gccggatgct tggctttcta caggattcga gcgccgatca tgggacccta
     1381 taacgcggaa ggaatccctc ctcgaatcat gaaacaatct ttctttgtca gggggcattt
     1441 ccttggctct agagaaattc ccacctatta caaatcctac gatggccaac ttaaaatcac
     1501 caattcctat gtcctattct gccccacttg cgccgaaatc tggggaagac tcatgcatga
     1561 acactggaat gctcactgcc aaattctccc tcgttactgc cgagaacacg ccagattccc
     1621 tgatgacgga ttcttctctc tccgaggata cgaagacaaa cctgcctgcc tccccggaat
     1681 ggtactcgaa agtgactggc cacttgaagc tatccgttt
//
LOCUS       DATMTI010000433         4589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_737734, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000433 DATMTI010000000
VERSION     DATMTI010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737734"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VN039_07035"
     CDS             complement(<1..580)
                     /locus_tag="VN039_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HWV45757.1"
                     /translation="MNVREILEFAKKHRVQMVDLKFVDLPGVWQHMTIPVGELTEGLF
                     RDGSGLDGSSIRGWKAINNSDLLVVPDPATAFLDPFTAVPTLSLTGNVVDPISRENYE
                     RDPRFIAQKAEKYLQSTKIGDNSFWGPEAEFFIFDHARYDQTSHSGFYYIDSEEGAWN
                     MGQEGINLGGKIRHKQGYFPVAPADTQQDIRSE"
     gene            complement(640..978)
                     /locus_tag="VN039_07040"
     CDS             complement(640..978)
                     /locus_tag="VN039_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWV45758.1"
                     /translation="MKLVEAIVKPFKLEEIKDALLEIGIQGMTVSEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEVAVTDAQVSRVIEAITNAAKTGSIGDGKIFVRELNEVVRI
                     RTGETGETAL"
     gene            complement(1337..3121)
                     /locus_tag="VN039_07045"
     CDS             complement(1337..3121)
                     /locus_tag="VN039_07045"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWV45759.1"
                     /translation="MRTLRIAMAQMNPTVGELIGNIRRITTWLRDAKEAKADLVVFPE
                     LAITGYPPEDLLLKAQFVADNLRALNEITQACRGLVAVVGYVGQSDQRDTHSSRPSIV
                     SACQHTLYNAAALIADRKLVGSYSKWYLPNYGVFDESRYFRPGRRLPLLLVNGTTIGV
                     TICEDIWLPEGPTRVQAAAGAEVIVNINASPFHVGKSRLREQMLATRARENGVIITYT
                     NMVGGQDELIFDGNSLILDQSGNVIARGHAFREELLIADLNVDAASHGRRPYGRKAAL
                     TGKAVSAVDRLVVNETAINKKKRTRIVPGVAKPLDEIEEVYRALVLAVKDYVRKNGFT
                     RVVIGLSGGVDSAVTAAIAADALGAKHVLGVFMPSPYTSHESGEDVTELARCLGIELS
                     TIPITPTFEAYRQSLAPSFVDCSEDATEENIQARIRGNLLMALSNKFGHLVLATGNKS
                     ELSVGYSTLYGDMAGGFAVIKDVPKTMVYGLARFRNELGPSPVIPKRILDRPPTAELR
                     PNQKDEDSLPPYAVLDPILRAYVEEDRSLDEIVAAGFDRATVSRVVRLVDSSEFKRRQ
                     APIGVKITHRAFGKDRRMPITNGYRIKP"
     gene            complement(3220..4329)
                     /gene="aroB"
                     /locus_tag="VN039_07050"
     CDS             complement(3220..4329)
                     /gene="aroB"
                     /locus_tag="VN039_07050"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWV45760.1"
                     /translation="MTPTSTVKVSLPERSYEIIIRAGLLERLGRELKRYGVKGKVGVV
                     TDRHVARHYLKQISESVRRYEIEPVPIILAPGERSKTLGTVEHILDMLARHRFERSSM
                     LLALGGGVVGDMAGFAASIYQRGISYIQVPTTLVAQVDSSVGGKTGVDHRLGKNLIGS
                     FYQPCAVWVDPLTLHTLPPRELVAGLAEVIKYGIIADEPFFAYLQRHMSELRRQAPGI
                     VATVVKRSCEIKAEVVAADERESDRRRILNYGHTIGHALEALGGYKAFVHGEAVGIGL
                     VQEAELACFQGICARTVVEEIRRTVNEAGLSDRMPRWAPQRIWKAMLHDKKVSEGRVI
                     GVWPERIGQVRIGPIEKHVFEQWYSVARVSPHGHA"
BASE COUNT         1029 a         1399 c         1097 g         1064 t
ORIGIN      
        1 tctcgctccg gatatcttgc tgggtgtcgg ccggagccac ggggaaatac ccttgcttat
       61 gccgaatctt cccccccagg ttgatcccct cttgccccat gttccaggcg ccttcctccg
      121 agtcgatata atagaagccg ctgtggctgg tttggtcgta acgggcatga tcgaagatga
      181 aaaattcggc ttccggtccc cagaatgaat tgtcgccgat cttggtactc tggagatatt
      241 tctcagcctt ttgcgcgatg aacctgggat cacgttcgta attttcccga gaaatcggat
      301 ccacgacatt tccggtcagg ctgagcgtcg ggaccgcggt gaagggatcc agaaaggccg
      361 tggctgggtc cggcaccacc aacaagtcac tgttgttgat agccttccag ccgcgaatcg
      421 acgatccatc aaggccggac ccgtccctga acagcccttc cgtcagctcg cccacaggga
      481 tggtcatatg ctgccacaca cccggcagat cgacgaactt cagatcgacc atctggaccc
      541 tgtgcttctt ggcgaactcc aatatctcac gcacgttcat gcccgtcctc ctttgacaga
      601 tcctccgcga tcatgttcca ccgcttgaag cctttaccgt catagcgcgg tttctccggt
      661 ttctcccgtc cgaatgcgta cgacctcgtt caactctcga acaaaaattt tcccatcccc
      721 gatgctaccg gtcttcgccg cgttggtgat ggcctcaatc acccgagaca cctgggcatc
      781 cgtcacagct acctcgattt tcactttagg gacaaattcg atcgtatact cttggcctcg
      841 ataggtttcc ttatggcctt tctgacgccc aaaccctttg acttccgata cagtcatccc
      901 ctggatgccg atttccaata gggcatcttt gatctcttcc agtttgaacg gtttgacgat
      961 ggcctcaacc agcttcatat cgatgcctcc ttgcccgacc agctcaggaa tccccgacgg
     1021 ctctcagatg cccatgaagg agccgtgcag agctaattga tgaaccatgc ctctcctcgc
     1081 ggaaaagcca tggcccctcc ggctttccct cagctttggg gcagccatca tagcccatcc
     1141 tttcctctca ctcaacgtgg aaatcctgta aatccttcaa aattgctcga cctctttatg
     1201 tcccacgctc tgtgcattga gcggttacag caatgcgatc caagggcgaa gcagccttgg
     1261 caattcattt tcatccggaa tccaggataa atgtccgaac agtaatgatg tgcttggaat
     1321 gcagaaccag tggaggttat ggcttgattc ggtagccatt cgtaatcggc atccgacgat
     1381 ccttgccgaa ggcgcggtga gtgatcttga cgccaatagg tgcttgacgt cgcttgaact
     1441 cgctgctgtc gaccagccgg accacgcggg agacggttgc gcgatcgaaa cctgccgcga
     1501 cgatttcgtc gagcgagcga tcttcttcga catacgcccg aagaatggga tcgagcacag
     1561 cgtacggtgg aagggaatct tcatcttttt gattcggtcg caactcggcg gtcggcggtc
     1621 ggtccaagat acgtttggga atcactggcg atggaccgag ttcattacgg aacctcgcca
     1681 ggccgtagac catcgtcttc ggcacatcct taatgacggc gaacccaccg gccatatcgc
     1741 catacagggt ggaatatccc acactcagct cgctcttgtt cccagtggcc aaaaccagat
     1801 gcccgaactt attggataga gccatgagca gattgcctcg aatacgagcc tggatgtttt
     1861 cctcggtcgc gtcctccgaa cagtcgacaa atgacggtgc cagcgactgt cgataggctt
     1921 caaatgtcgg agtaatcggg atcgtgctca actcgatgcc gaggcaccgc gcgagttctg
     1981 taacatcctc tccgctttca tgtgaggtgt atggagacgg catgaagacg ccgagcacgt
     2041 gcttggcacc aagcgcatcc gcggcaattg ccgccgtcac tgccgaatcg acgccgccgc
     2101 tcaacccaat cactactcgc gtaaatccat tctttcggac ataatctttc acggccagta
     2161 ccaaagcgcg gtacacctct tcgatctcat ccaacggctt tgctacccct ggcacgattc
     2221 gagtccgctt ctttttgttt attgccgtct cgtttaccac gagacgatcg acggccgaaa
     2281 cagcttttcc ggtcaatgcc gcctttcgcc cataaggcct acgtccatgt gaagccgcat
     2341 ccacgttcaa atcagctata agcagctctt cccgaaacgc gtgaccacgt gcgatcacat
     2401 ttccggattg atccagaatc aaactgttcc cgtcgaagat cagctcgtcc tggcctccaa
     2461 ccatgttcgt atacgtgata atgactccgt tttcccgcgc tctcgtggcc agcatctgct
     2521 ccctcaaacg acttttgccg acatgaaagg gagaggcatt gatattgacg atcacctcgg
     2581 ccccagccgc cgcttgtacg cgagtaggcc cttccggcaa ccaaatatct tcgcagatgg
     2641 tcacgccgat tgtcgtgccg ttgacgagga gcaacgggag tctccgtccg ggacgaaaat
     2701 aacggctttc atcgaatact ccgtagttcg gcaaatacca tttgctataa ctaccgacta
     2761 acttgcgatc ggcaatcaac gctgccgcat tgtaaagcgt atgctgacag gctgagacga
     2821 tcgacggtcg agaggaatga gtgtcgcgtt ggtcgctctg ccccacgtac cctaccacgg
     2881 caaccaaccc gcgacaggct tgggtgatct cgttcagggc ccgtaagtta tctgccacaa
     2941 attgcgcctt gagaagtagg tcttcgggag gataaccggt aatggccagt tcaggaaata
     3001 caaccaggtc ggctttggcc tccttcgcat ctcgaagcca ggtcgtgata cggcggatgt
     3061 tgccgatcag ctctccgaca gttgggttca tctgtgccat cgcgattcgc aacgtccgca
     3121 tagcgctccg gtaagcctgt cctatccccg atggtccagt gcactctaca ccgaagatag
     3181 gttcatatcg acttcgtccc ttggacagga gctgcgtaat taggcgtgac catgaggaga
     3241 gactcgtgca accgaatacc actgctcaaa cacatgtttt tcaatcggcc ctatccgcac
     3301 ctgtccgata cgttccggcc acaccccaat gactcgcccc tctgacacct tcttatcatg
     3361 cagcatggcc ttccatatcc tctgcggtgc ccaccgtggc atacggtcac tcagtcccgc
     3421 ctcgttcacc gtgcgccgaa tctcttcgac cactgtacga gcacagattc cttgaaaaca
     3481 ggccagttcg gcttcctgta ctaatccgat ccccaccgct tctccgtgga cgaacgcctt
     3541 atatcccccc aacgcctcaa gagcatgtcc gatggtatga ccgtagttaa ggattcgcct
     3601 gcgatcggat tctcgctcat ccgcggcgac cacctcggcc tttatctcac aggatcgctt
     3661 caccactgtc gcaacgatgc caggcgcctg cctcctcagt tcggacatgt gccgctgcag
     3721 ataggcaaag aacggctcat ccgcaatgat cccgtactta atgacctcgg caagcccggc
     3781 gaccaattcc cgagggggca aggtatgaag cgtcaatgga tctacccata cggcgcatgg
     3841 ttgataaaac gatccgatca ggttcttccc gagccgatga tcgacacccg tctttcctcc
     3901 gacgctggaa tcgacctgtg caacaagagt cgtcggcacc tgaatgtagg aaataccacg
     3961 ctggtagatc gaagcagcaa acccggccat atcgcccacg acaccaccgc cgagcgccaa
     4021 gagcatagac gaccgctcaa atcgatgacg cgccaacatg tcgaggatat gttccactgt
     4081 ccctaacgtt ttcgatcgtt cccccggtgc aagaatgatc ggcaccggct caatctcata
     4141 tcgcctaacc gactcagaaa tctgttttag ataatgccga gcgacatgtc gatccgtcac
     4201 aactccgacc ttccctttta ctccatatct cttcaattcc cttccgagcc tctctaaaag
     4261 tccggctcgg atgataatct cgtagctcct ttcaggtagg gaaaccttaa ccgttgaggt
     4321 tggagtcatt tcgtaaccca atcgctgacc gaacctgata atttcgtgtc atgtccattc
     4381 gggagccaca catgaaacat cgaagggaga agcatcgtaa cacagccccg atgaactaaa
     4441 aagacggtca cgtttcggga cgagtgccac aactctgggc ggtattccca tgatcaccgg
     4501 aaaacaaaaa agcaaaatcc cagtgatgct gaagcgcgtc accaggcttt tgctgatcac
     4561 ggagatgaaa caggtcctag actttaata
//
LOCUS       DATMTI010000434         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_568061, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000434 DATMTI010000000
VERSION     DATMTI010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568061"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VN039_07055"
     CDS             complement(<1..1230)
                     /locus_tag="VN039_07055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV45761.1"
                     /translation="MESGNGPDFSKGVSTDRLSDGGMLLGHIGQEDVILARRGGEFFA
                     VGATCSHYSGPLVEGLMLNDELRCPWHHACFSLRTGEALRAPAFDAIPCWRVERIGET
                     VFVREKLSAPGRARTSVSPQKAPASVMIVGGGAAGFAAAEMLRCEAYEGRVTIISADD
                     AAPYDRPNLSKDYLAGTAQKEWIPLRPPDFYGEHRIDLLLNSRVASLDAKRKSLRLDD
                     GRSFEFDALLLATGAEPVKLSIPGASDAQLHYLRTFADSRTLSEKAASAKQVLIIGAS
                     FIGLEVAASLRARGVAVHVVAPEQQPLEQVMGGEVGRFIRGLHEANGVVFHLGETVTR
                     VDGRKATLSGGGALETDLIIVGVGVRPSIALAEQAGLKTDRGVVVDEYLQTSVPGIFA
                     AGDIARWPDPHSGGLVRI"
     gene            complement(1409..>2213)
                     /locus_tag="VN039_07060"
     CDS             complement(1409..>2213)
                     /locus_tag="VN039_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWV45762.1"
                     /translation="IIARMAKATLGNRSRVDWAALEADYDRIRDHISRVIPGFERFNE
                     RIRKDIFYLPNSARDRKFITKTGKANFTVHTIPQWDLAPGELLMMTIRSHDQYNTTIY
                     GLDDRYRGIYGGRRVIFLNQEDMDDRGLKAGQLVDITSHFEAEERVVRRFQVVPYNIP
                     RRCAAAYYPETNVLISVRSVDGRSDQPAFKSVRITLARSSSKGYNPVAQILDQERVTV
                     IENLQKSSASLSRKTCFIEFLPRHDRPPYSRKQSSMRTCLNSSRILSAQ"
BASE COUNT          466 a          629 c          639 g          479 t
ORIGIN      
        1 gatacgaacg agccctccgg aatgtgggtc gggccaacga gcgatgtcgc ccgcggcaaa
       61 gatgcccggc acgctggttt ggagatattc gtcgaccaca acgccgcgat ccgttttcaa
      121 tccggcttgc tcggcaagcg caatcgacgg ccgtacgccg actcccacga taatcagatc
      181 tgtttcgagc gcgccgccgc cactcagtgt tgctttgcgt ccgtcgaccc gagtgaccgt
      241 ttcgcccaag tggaagacca cgccgttcgc ctcgtgaagg ccgcgaataa accggccgac
      301 ctccccgccc atcacctgct cgagcggctg ctgctccggg gcgaccacgt ggacggccac
      361 gcctcgtgcg cgcagggatg cggcgacttc gagtccgata aaactcgcgc cgatgatgag
      421 gacttgtttc gcggacgcgg ccttttcgga caaggtccgg ctgtcggcaa acgtgcgaag
      481 gtaatggagc tgagcgtcgg atgcgccggg aatggataat ttgacaggtt cggctccggt
      541 cgcgagtaaa agagcgtcga actcgaagga cctgccgtcg tcgagccgaa gagatttgcg
      601 ttttgcgtcc agtgatgcga cgcgtgaatt gagaaggaga tcgatgcggt gctcgccgta
      661 aaaatccggc ggtcgcaatg gaatccactc cttctgggcg gtaccggcga gatagtcttt
      721 ggacagattc gggcggtcgt acggagccgc atcgtccgca cttatgatgg tgacacgccc
      781 ttcgtacgct tcacaccgga gcatctctgc cgccgcgaag ccggcggcgc ctccaccgac
      841 gatcatgacc gaggcgggcg ctttttgcgg ggaaacggat gtccgcgccc gaccgggtgc
      901 agacagcttt tcccgcacga atacggtctc gccgattcgt tcgacacgcc aacatggaat
      961 ggcgtcgaac gccggggcac gcagcgcctc tcccgtacga agactgaagc aggcgtgatg
     1021 ccatggacag cgcaactcgt cgttgagcat caatccttca acgagcggcc cgctgtagtg
     1081 cgagcacgtg gctccgactg cgaagaattc gcctccgcgg cgcgcgagga tcacgtcctc
     1141 ctggccgatg tgtcccagaa gcattcctcc atcggaaaga cgatctgtcg agactccctt
     1201 gctgaaatcc ggtccgttcc cgctttccat atttcacctg tcagaatgtt gaatacccag
     1261 ctcggttcac ttaactttta ctgtaatgtt gtcggtcacg gcaagatcgt tgtggtcttt
     1321 tggtgagaga tacgaattgt attggagcgt gagccggggg taactaggca ttcccccaaa
     1381 gcgatgttat gttcagacat acatgagctt attgggccga caatatgcgt gaagaattca
     1441 agcacgtgcg catagatgac tgcttccgcg aataaggagg ccgatcgtgg cgaggaagga
     1501 actcgatgaa gcaggtcttt cgtgacagac ttgcagaaga cttctgcaag ttctcgataa
     1561 cagtaacgcg ttcctgatca agtatctggg ccacagggtt gtatcccttc gacgatgacc
     1621 gagctagagt aattcgaacc gacttaaacg caggttgatc gctccggccg tcaacgctcc
     1681 ggactgatat gaggacgttg gtttccgggt aataggctgc cgcgcaccgc ctgggaatgt
     1741 tgtacggcac aacttgaaat cggcgtacca cgcgttcctc ggcttcgaaa tggcttgtga
     1801 tgtccaccaa ctgccccgcc ttcaaccctc tgtcatccat atcttcctgg tttaggaaaa
     1861 tgacccgacg accgccgtag attccgcgat accggtcatc caacccatag atcgtcgtgt
     1921 tgtattgatc gtgactgcgg atcgtcatca tgagtaactc tcctggcgcg agatcccact
     1981 ggggaatggt gtggacggtg aaattggcct ttcctgtttt ggtgatgaac ttgcgatctc
     2041 gtgccgagtt cggcaaataa aaaatatcct tgcggatgcg ctcgttgaac ctttcgaaac
     2101 cgggaatgac cctggagata tggtcacgaa tgcgatcgta gtcggcctca agggctgccc
     2161 aatccaccct gctgcgatta cccaaggtgg ccttggccat ccgtgcaata ata
//
LOCUS       DATMTI010000435         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_230109, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000435 DATMTI010000000
VERSION     DATMTI010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230109"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..992
                     /locus_tag="VN039_07065"
     CDS             183..992
                     /locus_tag="VN039_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45763.1"
                     /translation="MIKEHPILTAHGSFADHVKLGRKPKREDHRTLELAKYLKKDVVL
                     PTIPASCDYTKDVTEWPMYGNDSMGDCTCAAAGHMIQGWSAAAGAAKTPADNDVITLY
                     AETGDPPSSTITPGSPQDDGRDEMSVLNFWRQNGLGTDKIEAYVSVDPSDEQMVKAAI
                     YLFGGVYTGIALPITAQSQSVWDVVDAPPDQNEPGTWGGHAVPYLAFDGTSYTCITWG
                     HPLQLTKAFHAEYTDEVYAVLSSDFLAANSLSPDGFDLNALRADLLEVSSN"
     gene            992..>2081
                     /locus_tag="VN039_07070"
     CDS             992..>2081
                     /locus_tag="VN039_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45764.1"
                     /translation="MAPVGRPRVTTRQGTRAPTTEIGVPDLGLGGWGVVGAPSPLQGG
                     NGPWRMYVDEWEYVPELRWPLSVRLFDQMRTDEQLAGLLTAVMWGICQLRFVVDPNGA
                     RDSLVKEIAQDLNLPILGKDPEPIGRMKKRFSHSRFVTQAMLSTIYGHTYFEQVGEIV
                     DGKWRLRKLAPRMPQTIRQINVAEDGGLVSIVQWAPIGWNFQAQQQQPGYFQGPEIPV
                     DNLVAFIFQQEGMSWTGRSMMRDCYRPWIIKDRDLRIEAVNHERAGGVPYAEGAMGMT
                     DDELKDLNDLMSQFRIGENSGLAVPSGTKVNIARGTGSDVDKTIKRLDEAMARRFLLQ
                     LVNLAQGGSHVGSYSLSETFEDFFLVGQR"
BASE COUNT          424 a          583 c          634 g          440 t
ORIGIN      
        1 atttcaacgc ctggctagcg ggaggactgc tcgcctatgg gctgagcatt ctcgtgccgg
       61 tatttcaaac tacgagtctg gaggtcaatc gtggctggta aggcaggtct caccaagccg
      121 acgggcaaga cgtccgtcac caacatgaag aacgttccga ctgctccgag gatgactcac
      181 aagtgatcaa agagcatccc attctcacgg cacacggctc tttcgccgac cacgtcaagc
      241 tcggccggaa gccgaagcgt gaagaccacc gcacgcttga gcttgccaag tatctgaaga
      301 aggacgtcgt tcttcccacg atccccgcga gttgtgacta caccaaggac gttactgagt
      361 ggccgatgta cggcaacgat tccatgggcg attgcacctg tgctgcggca gggcacatga
      421 tccagggctg gtcggccgct gcgggtgcgg ccaagacgcc agcagacaat gacgtcatca
      481 cgctctacgc ggagacgggt gatccgcctt catccacgat cacgccgggt agtccgcagg
      541 acgacggtcg cgatgagatg agcgtcctca atttctggcg gcagaatggg ctgggaacgg
      601 ataagatcga ggcttatgtc tctgtcgatc cttctgacga gcagatggtc aaggctgcga
      661 tctacctgtt cggtggcgtc tacacgggca tcgcactgcc catcacggcg cagtcgcagt
      721 ctgtctggga cgttgtagac gctccgcccg accagaatga gcccggtacc tggggcgggc
      781 atgctgtgcc gtatctggcg tttgacggca ccagctatac ctgcatcacc tggggtcatc
      841 ctctgcagct gaccaaggca ttccacgcag agtatacgga tgaggtctac gccgtgctct
      901 cctcagactt ccttgctgca aacagcctct ctccggacgg atttgatctc aacgcgctac
      961 gggcagattt gctggaggtc agtagcaact aatggctccg gtcggtcgtc ctcgggtcac
     1021 aacgcgccag ggcactcgtg ctccgacgac tgagatcggt gtccccgatc ttggcctcgg
     1081 agggtggggc gttgtcggtg cgccatctcc gctgcaggga ggcaatggac cttggcgcat
     1141 gtacgtggac gaatgggagt acgttccgga gcttcgttgg ccgttgagcg ttcggctctt
     1201 cgaccagatg cgtacggacg aacagctggc aggtcttctc actgccgtga tgtggggaat
     1261 ctgtcagttg cggttcgtcg tcgatccgaa tggtgctcgg gattctctcg tcaaggagat
     1321 tgcccaggat ctcaatttgc cgatccttgg gaaagatcct gagccaatcg gacgcatgaa
     1381 gaagaggttc tcgcacagtc gcttcgtgac gcaggccatg ctctccacaa tctatggtca
     1441 cacctacttc gagcaggtgg gcgagatcgt ggacggaaag tggcgcctca ggaagctagc
     1501 cccgcgcatg ccacagacga tccgccagat caacgtagcc gaggatggcg ggttggtgag
     1561 catagttcag tgggcaccaa ttggctggaa ctttcaggcc cagcaacagc agcccggcta
     1621 cttccagggt cctgagattc cggttgacaa tctcgttgca ttcattttcc agcaggaggg
     1681 aatgtcctgg acgggtcgca gcatgatgcg cgactgttac cgtccctgga tcatcaagga
     1741 tcgtgacctt cggattgagg cagtgaacca tgaacgcgcc ggtggcgttc catatgccga
     1801 gggtgcgatg ggcatgacgg atgatgagct aaaagatctc aacgatctta tgtcccaatt
     1861 ccgcatcggc gagaattcag gtctcgcggt accgtccggc acgaaggtca atatcgcacg
     1921 cggtacgggc agcgacgtgg acaagacgat caagcgtctt gacgaagcta tggcccgtcg
     1981 cttccttctg cagctagtca atctggcgca gggagggtct cacgtcgggt cgtactcgct
     2041 gagtgagacg tttgaagatt tcttcctggt tggtcaacgc c
//
LOCUS       DATMTI010000436         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_398839, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000436 DATMTI010000000
VERSION     DATMTI010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398839"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VN039_07075"
     CDS             <1..309
                     /locus_tag="VN039_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45765.1"
                     /translation="DPMDADTLLETYLYQFHMDPTALHESHPHFVWNTDEATARFHHL
                     ARVESLKQEFQYLDPNGLLDQIKQHHLNTFSPSRHSQRVELAASLRSKRLSDLAWNNR
                     "
     gene            306..1532
                     /locus_tag="VN039_07080"
     CDS             306..1532
                     /locus_tag="VN039_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45766.1"
                     /translation="MTTFTSRWGFPQIVISPTSDNVDVVTDFNTPWATLDTKLYGVCT
                     SSTRPSAPVQGQHTFETDTGFTRVWTGSAWKTAGNAIGTSGARPASPIQGDEFYETDT
                     GYSRSRGSAAWNGFLPVCTVSTLPANPIAGDEVYLSDKECTARYTGTQWHTIGPVVCT
                     SSTHPTSSGITLYSGMTIYETDTSRFLIYNGTTFIVQTSANFVCTSTTHPANPFVGLE
                     IYETDTGLSAVYTGSAYYYPPQLWQSQTVVGTTTGTLTFNNIPQVGKTLKVVMRVKTA
                     AATTFDTILYTINNTTSANYNFFIQSITNAGAPTNQNATSQTGGTCAIAWGNGTATNG
                     AGLNEVLFPDYSIAGFAKGCRFSGFASDGNTSGESWQGGSMLKSTITTAAITRLDFTT
                     GSGNYTAGSTIDVYIM"
     gene            1544..1840
                     /locus_tag="VN039_07085"
     CDS             1544..1840
                     /locus_tag="VN039_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45767.1"
                     /translation="MQIVITADDIFGEYKKHVSDLTHELIMEKAKVAKLLAIVEENEK
                     LAATRALPMRQLGQPAAAPQTAGNMPEPARLPESVPEPPPLPPGPDSGPSRPGT"
     gene            1865..2206
                     /locus_tag="VN039_07090"
     CDS             1865..2206
                     /locus_tag="VN039_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45768.1"
                     /translation="MKVFAGISVLQGGAAVLLALGVYLILTGKLVWYKNAQKQLDQVT
                     EICNERVKQANEETGRWRTAYEFSESARQVQTQHTNELLQIGRTTNRVIAALSPSPEG
                     EPDATASVVER"
     gene            2178..2444
                     /locus_tag="VN039_07095"
     CDS             2178..2444
                     /locus_tag="VN039_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45769.1"
                     /translation="MRLHLLWKDRRRKNGGEEQPDTHRGIAEADAAVRRAEQKYEEAK
                     SQKGEVDSVVRQLKERQRVNHFRDAVVRSLQEGYGPQDRGHQHG"
BASE COUNT          605 a          731 c          694 g          469 t
ORIGIN      
        1 gatccgatgg atgcagatac gttgcttgaa acctatctgt accaattcca tatggacccg
       61 acggcgctac atgaaagcca ccctcacttc gtatggaaca ccgatgaagc cactgctcgg
      121 tttcaccacc tggctagagt agaaagtcta aagcaggaat ttcagtatct tgacccgaat
      181 gggctgctcg atcagataaa gcagcaccat ttgaacacct tcagtcccag taggcattcg
      241 cagcgtgttg agctagctgc aagcttaagg tcgaaaagac tgtccgatct cgcttggaat
      301 aacagatgac tacatttact tcccgatggg gctttccaca gattgtcatc agtcccacgt
      361 cggacaatgt cgatgtggtc actgacttca acactccttg ggccacactg gacaccaagc
      421 tctacggcgt ctgtaccagt tctacacggc ccagcgctcc ggtacagggt cagcacactt
      481 tcgaaacgga caccggtttc actcgcgtct ggactggctc agcgtggaag acggcgggga
      541 acgcgatagg aacttccgga gcgcgcccgg ccagtccgat ccagggtgac gagttctacg
      601 agactgacac cggttactca cgctctcgtg gttctgccgc atggaacggt ttccttccgg
      661 tctgcacggt gagtacgctc cctgcgaacc ccatcgcggg cgacgaggtc tacctctccg
      721 acaaggaatg caccgctcgc tataccggca cacagtggca cacgatcggg ccggtagtct
      781 gtaccagcag cacccacccc acgtccagcg gcatcacctt gtacagcggc atgacaatct
      841 acgaaacgga cacttcccgt ttcttgattt acaacggcac cacattcatc gtgcagacat
      901 cggcgaactt cgtttgcacc agcacgacac acccggccaa cccgttcgtc ggtttggaaa
      961 tatacgagac ggacaccggt ctctctgcgg tctacaccgg aagcgcgtat tactacccac
     1021 cgcagctctg gcagtcccag accgttgtcg gcacgactac gggaacgctt accttcaaca
     1081 acatcccgca ggtcggtaaa acactgaaag tggtcatgcg ggtgaagacg gcggcggcga
     1141 ccaccttcga caccatttta tatacaatca acaacaccac gtctgcgaac tacaacttct
     1201 ttattcagtc gatcacgaat gccggtgccc ccactaacca gaacgcgacc tcgcagaccg
     1261 gtggtacctg cgcgatagca tgggggaacg gcacagcgac caacggtgcc ggtttgaatg
     1321 aagtgctctt ccccgattac agcatcgctg gtttcgccaa gggctgccgc ttctccgggt
     1381 tcgccagcga tggcaatact tccggggagt cgtggcaggg tggctcaatg ctgaagtcca
     1441 ccataactac ggccgcaata acccgtcttg acttcaccac cgggagcggg aactatactg
     1501 ctggttccac catcgacgtt tacatcatgt gagagaaaag ctaatgcaga tcgtgatcac
     1561 ggcggacgac attttcggcg agtacaagaa gcatgtttcc gatctcaccc atgaactgat
     1621 catggagaag gcgaaagtag caaaacttct ggccatcgtg gaagaaaacg aaaagctggc
     1681 agccacgcga gcgcttccca tgcggcagct cggtcagccc gcagcagccc cgcagacggc
     1741 cgggaacatg ccggagccag caagactgcc cgagagcgtt cccgagccgc caccgttgcc
     1801 cccagggccg gacagcgggc cttccaggcc gggcacctag cagtaaggcg acagttcaga
     1861 aggggtgaag gtgttcgcag gcatttcagt cctccagggt ggtgcggcag tcctgttggc
     1921 gctgggggtt tacctgatcc tcaccgggaa actcgtttgg tacaagaacg cgcagaagca
     1981 gctcgatcag gtcactgaaa tatgcaacga gcgcgtcaag caggcgaacg aagagactgg
     2041 aaggtggcgt actgcttatg agttctcaga aagtgcacgc caggtccaga ctcaacatac
     2101 caacgagctt cttcagatcg gtcgtactac caaccgggtc atcgccgcac tatccccatc
     2161 ccccgaggga gaaccagatg cgactgcatc tgttgtggaa agatagaagg cgtaagaacg
     2221 gcggcgaaga gcagccagac acgcaccggg gcatcgccga agccgacgca gcagtgcggc
     2281 gggccgaaca aaagtacgaa gaggcgaagt cccagaaggg ggaggtagac agtgtggtca
     2341 gacagctcaa ggaacgacag cgggtgaatc acttccgcga tgcagtggtg cggtcactac
     2401 aggaggggta cggtccgcag gatagagggc accagcatgg ataacgccgc cattgaagca
     2461 tcagtgtggt tcgcgttcgc gggatcagtg tggttcatc
//
LOCUS       DATMTI010000437         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_823707, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000437 DATMTI010000000
VERSION     DATMTI010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823707"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..>1618)
                     /gene="nuoD"
                     /locus_tag="VN039_07100"
     CDS             complement(130..>1618)
                     /gene="nuoD"
                     /locus_tag="VN039_07100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HWV45770.1"
                     /translation="ARVTEDNPSLDSVTSVWRGATFLEREVYDMMGIRFSGHPDLRRI
                     LLPEDYAEGYPLRKDFPTEGRGWRSRFDFIPRLDEPPVEHIESEVPEAEKKLFRSEPT
                     GSSSNRREELLLNMGPQHPSTHGVVRVILELDGERIVKATPDLGYLHRGVEKLAEGLA
                     YMQIIPHTDRLDYVCAMANNYAYVRAVEKLLGITVPERAEYIRTIVAEMQRMLGHLFW
                     LGAQALDIGAMTIFFWTFRERETLLDMFERLCGARLTLNYYRIGGVDSDFTPELVAHL
                     KTFLETFPQKVNEYDSLLAGNRIWLGRTKQVAVISGEDAINFGMTGPALRGSGVSYDV
                     RKLEPYGVYHKVDWEVPVGKNGDTYDRYWIRVEEMRQSARIISQCLDQMPPGPIIADM
                     PQYIPPPKQQVMRDMESLIHHFIIFTQGFKPPKGETYCATEAPKGELGFFIISDGSPR
                     PYRLKIRSPSFIHMGAFDHMARGYLISDIITIFGTYDVVMGECDR"
BASE COUNT          342 a          478 c          446 g          352 t
ORIGIN      
        1 ccaggatgtt cccaactgcg cgcattgacc gagcacgtcg tttataccca cccgacccgc
       61 gtcgccaaga ctccgcgtca cccgagcttg cgcgggcagc gagcacggga acatcctggc
      121 cgtcacgcct catctatcgc actcccccat gaccacatcg taggtcccaa aaatcgtgat
      181 gatgtcggaa atcaaatagc cgcgcgccat atgatcgaac gcgcccatgt ggatgaagga
      241 gggcgaacga attttcaacc gataagggcg tggagatcca tcactaataa taaaaaatcc
      301 caattccccc ttgggcgctt cagtcgcgca ataggtttca cccttcggcg gcttgaaccc
      361 ttgggtgaag atgatgaaat ggtgaatcaa gctctccata tcgcgcatga cttgctgttt
      421 cggcggcgga atgtattgcg gcatatcggc gatgatcggt ccgggcggca tctggtcgag
      481 acattgtgag atgatccgtg cgctctgacg catctcttcg acgcgaatcc aatagcgatc
      541 gtaggtgtcg ccgtttttgc cgaccggcac ttcccagtct actttatgat agacgccgta
      601 aggttcgagt ttgcgtacat cataggacac gccggatcct cgcaaggcag ggccggtcat
      661 gccgaaattg attgcatctt ccccggagat gacggcgacc tgtttcgtcc gtcccaacca
      721 aatccggttg cctgcgagca gggaatcata ctcgttcact ttttggggaa atgtttccag
      781 gaaggttttg agatgggcta ccaattcggg agtaaaatcg ctgtccacgc cgccgattcg
      841 ataataattc aaggtcagcc tggctccgca cagccgttcg aacatgtcga gcaaggtttc
      901 ccgctcgcga aatgtccaga agaagatcgt catggccccg atatccagcg cctgagcgcc
      961 cagccagaac agatgcccca gcatgcgctg catctccgcg acaatggtgc ggatatattc
     1021 ggctcgttcg ggcacggtga taccgaggag cttctcaacg gcccgcacat acgcgtaatt
     1081 gttggccatc gcgcagacat agtcgagccg gtcggtatgg ggaatgatct gcatgtaggc
     1141 gagaccctca gccaatttct cgacgcctcg atggaggtat cccagatccg gcgtcgcctt
     1201 gacgattcgt tctccgtcca gctcgagaat gacccgcacc acgccgtgcg tgctcggatg
     1261 ttgtgggccc atattcagca acagctcttc ccgccggttc gatgaggacc cggtcggctc
     1321 ggaacggaac aacttcttct cagcctcggg aacttcgctt tcgatgtgct cgaccggcgg
     1381 ttcatcgagg cgcgggataa aatcgaaccg acttcgccac ccgcggcctt ccgtcgggaa
     1441 gtccttccgc agcggatatc cttccgcata gtcttccggc aggaggatgc ggcgtaggtc
     1501 ggggtgacct gaaaaccgga ttcccatcat gtcgtagact tcgcgttcta agaatgtcgc
     1561 gccgcgccag acgctcgtca ctgaatcgag cgacgggttg tcttcggtga cccgcgct
//
LOCUS       DATMTI010000438         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_101419, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000438 DATMTI010000000
VERSION     DATMTI010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101419"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..159
                     /locus_tag="VN039_07105"
     CDS             1..159
                     /locus_tag="VN039_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWV45771.1"
                     /translation="MAHCATVGDFTTRHLLEHMIEDSEEHVDWFETRLRTIKQVGLEN
                     YLSEQIKK"
     gene            complement(526..>1689)
                     /locus_tag="VN039_07110"
     CDS             complement(526..>1689)
                     /locus_tag="VN039_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45772.1"
                     /translation="WLPGEKAQAIAHIAKVVREDDGSFDLTDTGLSYRILFSGTNPDI
                     PEELRMPPQFRAFNGSATDQEIFDAVVKVVTAYVNGLRFSKTDDNGVPIRSPFDVFLA
                     INGLPQQPDPNESPLSYSQRLRALINAPGFSPQFVTSNPNRPDGQFQFHTQAFTFGAT
                     ELAGLRMFLSEPATLPASPAELTAGKIGNCIACHAAPDFTDFKLHNTGTTQSEYDSIH
                     GANAFGTLVIPNLAARNGNYDAFLPATEHHPNASERFRAIPTIADPTLVDLGVWNVFA
                     NPDIPNPQAKIRAILCDDQVPCPLSDSILLDRAIARFKTPGLRDPDHSAPFMHNGRLD
                     TLNDVINSYINRANEARAGALRNGDSRLQGIALKTNDIASLVPFLKSLNEDYQ"
BASE COUNT          368 a          397 c          530 g          394 t
ORIGIN      
        1 attgctcact gcgctaccgt cggtgatttc acgacgcgtc atctattgga acatatgatc
       61 gaagattctg aagagcatgt cgactggttc gagacacgac tgcgaacgat caaacaggtg
      121 gggctggaga actatttgag cgagcagatc aagaagtagg tgcctgtcct gtctgtttcg
      181 gctatacgat cggatgtctt ctccggctca cgggaggtga gccgggagac tccgggttac
      241 catgctgtcc gcatggcgaa aagccgcggt ctgagaagct atggattagg gaaaagtttt
      301 cagccatgct ttgagccgtc gcgttcggag cgatttcgcg agcccgcgtg cttgcccttg
      361 ctgacgattg aatagccagc agtttttgca ccagaggcgg gaactctggt gtcccgtcgg
      421 tctgttgcaa cggttgcaga tcagtgtctc agaatcggag cgggatgtgc gggattctgc
      481 ttgttgcatg ggaaaacagt tgttatctcg ataccagccg gttgattatt gataatcctc
      541 gttcagcgac ttgagaaatg gcaccaggga tgcgatgtcg ttggtcttga gcgcgatgcc
      601 ctgaagccgg ctatccccat tgcgcagcgc gcctgcgcga gcttcgtttg ctctgttgat
      661 ataggaattg atcacgtcgt tcaacgtatc caatcgaccg ttgtgcatga aaggagcgga
      721 atggtcagga tcgcgaaggc ctggcgtttt gaatctggcg atggcacgat ccagtaagat
      781 cgaatcggat aaagggcacg gcacctggtc atcgcacaag atggctctga ttttggcctg
      841 gggattcgga atgtcgggat tggcgaagac attccacacg cccagatcga ccaaggtggg
      901 gtcggcaatc gtcgggatgg ctcggaaacg ttctgaggcg ttgggatggt gctccgtggc
      961 agggaggaag gcatcgtagt tcccattacg agcggcaagg tttgggatta cgagagtgcc
     1021 gaatgcattg gcaccatgaa tgctgtcgta ctcactttgt gtcgtgccgg tgttgtgcag
     1081 cttgaaatcc gtaaagtcgg gtgcggcatg gcaagcaatg caattgccga tcttgcccgc
     1141 ggtaagctcg gccggtgaag cgggcagcgt agccggctcg gagaggaaca tcctcaagcc
     1201 ggccagttct gtcgcaccga acgtgaatgc ttgagtgtgg aactggaact gtccatccgg
     1261 tcggttgggg ttggatgtca cgaattgcgg ggagaagccc ggcgcattga tcagtgcccg
     1321 gagccgttgg ctataggaga gtggagattc gttcggatct ggctgctgag gtaagccgtt
     1381 gattgccagg aacacatcaa acggcgagcg aatcgggacc ccattgtcgt cggtcttaga
     1441 aaacctgaga ccgttcacat aggccgtgac taccttcacg actgcatcga agatttcctg
     1501 gtcggtggcc gatccgttaa aggcgcgaaa ttgaggaggc atgcgcagct cttcaggaat
     1561 atccggattg gtgccggaaa ataaaattct gtaagacaag ccagtgtcgg tcaaatcaaa
     1621 agaaccatcg tcttcccgaa ccacctttgc gatgtgtgcg atggcctggg ctttttcgcc
     1681 gggaagcca
//
LOCUS       DATMTI010000439         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_17057, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000439 DATMTI010000000
VERSION     DATMTI010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17057"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..>1632)
                     /locus_tag="VN039_07115"
     CDS             complement(79..>1632)
                     /locus_tag="VN039_07115"
                     /inference="COORDINATES: protein motif:HMM:NF009488.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HWV45773.1"
                     /translation="GPKGNNVVIAKGYGGPTVTHDGVTVAESVELPDTDDETLGYKVG
                     AELIKQAAKNLNKAAGDGTTTVTVLTYNILKEANRLIAAGHNPQELRKGIEAAGAEVV
                     KALDGLAESIEGKSDRVAEVATISAGDETIGKLIADVIEKVGKEGVVTVEAGQGLEME
                     AEVVEGFSFDKGWASPFFVTDTGRQEAVYEKPAIVLTDKKISTAQELLPLIEKLAQAG
                     KKDIVLIADEVEGEALSILVLNKLKGVLNTVVVKAPSFGDRRKDILADIAVLTGATVI
                     SEDQGLTFENVQLDVVGSARKVIASKDDTTIIEGGGRDADVKSRITQIAAQADNASSE
                     YDKEQYDKRAAALSGKVAVIKVGGATETEIDEKKFRVDDAVAATKAALAEGIVAGGGV
                     TLVNLAATITADGADSVSAGRQILKNALRTPFLQITANAGLNSEALLAQVEAAKSGFG
                     VNVMQPEDGLVDVVKAGVIDPARVTKQAVQNAVSIASTAATMGALVVDVPEKETPDMG
                     AMGGGMGMM"
BASE COUNT          321 a          499 c          403 g          409 t
ORIGIN      
        1 ttcatactga ccatcacgac gaacgcaaaa gacccgccgg tctcaccgac tcgcggggct
       61 cgtcctattt tgggcttctt acatcatacc catgccgccg cccatggccc ccatgtctgg
      121 agtttccttt tcaggaacat cgacgacaag agcacccatg gtggctgcgg tcgatgcgat
      181 ggaaacggcg ttttgtacag cctgcttcgt cacgcgagcc ggatcgatga caccggcttt
      241 gacaacatca accagaccat cttctggctg catgacattg acaccgaagc cagatttagc
      301 tgcctctact tgagctaaaa gcgcttcact gttcaagcca gcattggcag tgatttgaag
      361 gaacggtgtc cgaagagcat tcttcagaat ctgacgaccg gccgaaacgc tgtccgcgcc
      421 atcagccgta atcgtggccg ccaaattgac gagcgtcaca ccaccaccgg cgacgatacc
      481 ttctgcaagt gcggctttcg tcgcagccac ggcgtcatcg acacggaatt ttttctcatc
      541 gatttctgtt tccgtcgcgc caccgacttt gatgacggct acttttccag acagcgccgc
      601 cgcgcgctta tcgtactgct ctttgtcata ttcgctcgat gcgttgtcgg cttgagccgc
      661 gatctgtgtg atgcgactct ttacatcggc gtcacgacca ccgccttcga taatcgtagt
      721 gtcatctttt gaggcgataa ccttgcgcgc cgaaccgacc acgtcaagct gcacgttctc
      781 gaatgtcaga ccttggtctt cggaaataac agttgcaccg gttagcaccg cgatatcggc
      841 cagaatgtcc ttacggcgat cgccgaagct tggcgctttg acgacgacgg tgttaagcac
      901 gcccttaagc ttgttgagta ccaggatact gagcgcctca ccctcgactt catcggcgat
      961 caggacaata tccttcttgc ctgcctgagc cagcttctcg atcagcggca acagttcctg
     1021 tgccgtagag attttcttat cggtcagtac gatggcaggt ttctcgtaga ccgcttcctg
     1081 ccgaccagta tcggtcacga agaacggact agcccaacct ttgtcaaagc tgaaaccttc
     1141 gaccacttcg gcttccatct caagaccctg gccggcttcc accgttacga cgccctcttt
     1201 gccgactttc tcgatcacat cggcaatcag cttgccgatc gtttcatcac cggcggagat
     1261 agtcgcgact tccgccacgc gatcgctctt gccttcgata ctttcagcca gaccatcaag
     1321 cgccttcacg acttcggcgc cagcggcttc gattcctttg cgaagttcct gcggattgtg
     1381 gccggcagcg atcagacgat tggcttcttt caggatgttg tatgtcagga cagtcacggt
     1441 ggttgtaccg tcgccggctg ctttgttcag atttttggct gcctgtttga taagttcggc
     1501 gccgaccttg taaccgagcg tttcgtcatc cgtatcgggc aattcaacac tttcggcaac
     1561 cgttacaccg tcatgtgtga cggtcggtcc gccatagccc ttggcgatga ccacgttgtt
     1621 gcctttcggg cc
//
LOCUS       DATMTI010000440         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_399141, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000440 DATMTI010000000
VERSION     DATMTI010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399141"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /gene="gatB"
                     /locus_tag="VN039_07120"
     CDS             complement(<1..1122)
                     /gene="gatB"
                     /locus_tag="VN039_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWV45774.1"
                     /translation="MTYETVIGVEVHAQLRTKSKMFCGCGTTFGLSANSQTCPICLGL
                     PGSLPVINRTAVDMAVRAGLALNCTIAANNQFARKNYFYPDLPKGYQISQYESPICQH
                     GWIEIHDSSGKKRIRIRRAHLEEDAGKNIHETGTGGSRVDLNRAGTPLLEIVTEPDMG
                     SADEVVACLKGLREILMYLDVCDGNMEEGSFRCEPNLSLRPSGQKEFGTKVELKNINS
                     FKYVKDAVEYEIRRQTKVLNEGGTIRQETRLWNIERSETAVMRSKEEAHDYRYFPDPD
                     LVPLKLDDEWIEGFRADLPELPAVRTKRFVSDYGLPEYDATILTASKGMADYFEVCAK
                     QFTHPKTVSNWVMGELMRELNNSGTDISASPVTPERLVSL"
     gene            complement(1119..1442)
                     /locus_tag="VN039_07125"
     CDS             complement(1119..1442)
                     /locus_tag="VN039_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWV45775.1"
                     /translation="MADDRGTSAMVFLAFLSGATMGAVAALLLAPQSGDESRDQIRRY
                     ARRAENDLRDLAGRAGEAFEEVVDQGKEFVETKRSVLRDAFDAGREAVKRERGRIHDE
                     GPERG"
     gene            complement(1432..1839)
                     /locus_tag="VN039_07130"
     CDS             complement(1432..1839)
                     /locus_tag="VN039_07130"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HWV45776.1"
                     /translation="MTIVEIAALLVAVAFAVLVGYLVPVLMQVRKTVAESEQLLSKMN
                     AEVPALVAELRAMSQNLNDVTNQAREGVEHAAVLLHAVGEVGESVQQVHNVVRGSGGT
                     LLNRVASMIAGFRAASRVVRERMKQEGGTHNGG"
     gene            complement(1891..>3261)
                     /gene="gatA"
                     /locus_tag="VN039_07135"
     CDS             complement(1891..>3261)
                     /gene="gatA"
                     /locus_tag="VN039_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWV45777.1"
                     /translation="ISQVEPKVKAFVTQAKESASLQAEALDQKLKAWRKIAPLTGMPL
                     AIKDNICTNGVHTSCSSRMLQNFIPPYDATVVAKLRAQEYLLVGKTNLDEFAMGSSTE
                     NSAFGPSRNPWNLHCVPGGSSGGSAAAVAAEECVAALGSDTGGSIRQPAAFCGVVGLK
                     PTYGRVSRYGLVAFASSLDQIGPITRDVPDAAFLLQAIAGHDPMDSTSVDRSVPDYMK
                     SLQKRDLKTLKVGVPVEFFTEGLDSDVEQAVRAAIGELKNLGAEIREIRLPSTGAAVA
                     VYYVIATAEASSNLARFDGVKFGFRTKETKDLLELYMKTRQEGFGPEVKRRIMLGTYV
                     LSAGYYDAYYGKAQAVRTLVCQDFAAAFKEVDLIVTPATPTPAFKLGEKSEDPLQMYL
                     SDIFTISVNLAGLPAIALPCGFSKTGLPIGLQLIGRAFEEETVLRAAHAYEQSTHWHL
                     KKPMIR"
BASE COUNT          674 a         1044 c          795 g          748 t
ORIGIN      
        1 aagactcacc agccgttcag gcgtgacagg tgacgctgaa atatccgtcc cggagttgtt
       61 caactctctc atcaactctc ccatcaccca attgctcacg gtcttggggt gagtgaactg
      121 ctttgcgcaa acttcaaagt agtccgccat gcccttcgaa gccgtcagaa tggtggcgtc
      181 atattcaggc aacccatagt ccgacacaaa tcgcttcgtc cgcacggccg gcagttcggg
      241 caagtcggca cggaatcctt caatccactc atcgtccaac ttcaacggca ccagatcagg
      301 gtcggggaag taccgatagt catgggcttc ttctttcgaa cgcatgaccg ccgtttcact
      361 acgttcgatg ttccagagcc tcgtttcctg acgaatcgtg cctccctcat tcaacacttt
      421 cgtctgccgc ctgatctcat actcgaccgc atccttcaca tacttgaagg agttgatgtt
      481 cttcagctcg actttcgtcc caaactcctt ctgccccgac ggacgcagcg acagattcgg
      541 ctcacaccgg aagcttcctt cctccatgtt gccgtcgcag acgtcaaggt acattaagat
      601 ctcccgcagc cctttaaggc aagcgaccac ctcgtcagca gaacccatat ccggctccgt
      661 cacgatctcc aacagcggtg taccggcgcg gttcagatct acccggctcc caccggtgcc
      721 ggtctcatgg atatttttcc ccgcatcctc ttccaaatgc gcgcgacgga tacggattcg
      781 ttttttcccg ctactatcgt gaatctcaat ccacccatgc tggcaaatcg gggactcata
      841 ttgggaaatc tgataccctt tcggtaaatc cgggtagaaa tagttctttc tcgcgaattg
      901 gttgttcgcc gcgatcgtac agttcagtgc taaacccgca cgaaccgcca tgtcgaccgc
      961 cgttcgattg atcacgggca aactgcccgg caggccgaga caaattggac aggtctggct
     1021 gttggccgac agaccaaatg ttgtgccgca cccgcagaac attttggact tcgtccgcag
     1081 ttgtgcatgc acttccaccc caatgaccgt ttcgtaggtc atccgcgctc cggaccttcg
     1141 tcatgaatac gaccgcgttc gcgcttcacg gcttcgcgcc cggcatcgaa tgcatctcgc
     1201 aaaaccgacc gcttcgtctc aacaaattcc ttgccctgat caacgacttc ttcaaatgcc
     1261 tcaccggccc gtccggcaag gtcccgcaga tcgttttccg cgcgacgggc atatcttcga
     1321 atctggtcgc gtgattcgtc ccccgattgc ggcgccagca agagcgccgc gacggcgccc
     1381 atcgtcgctc cgcttaaaaa tgccaagaaa accatcgctg atgttcctcg atcatccgcc
     1441 attgtgtgtc cctccttctt gtttcatgcg ttcccgcacg acgcgagagg ccgccctgaa
     1501 acccgcaatc atgctcgcca ctctgtttaa gagcgtccct cccgatccgc gaacgacgtt
     1561 atggacctgt tgcacagact caccgacttc acccactgcg tgaagcaaga ccgccgcgtg
     1621 ctcgaccccc tcgcgcgcct ggttcgtcac atcgttcaaa ttctggctca tggcccgcag
     1681 ttcagccacg agagccggca cctcggcgtt catcttcgag aggagctgtt cagactcggc
     1741 caccgtttta cggacttgca tcaataccgg cacgagatac ccgaccagca cagcaaatgc
     1801 caccgccacg agaagcgcgg cgatttcgac gatcgtcata gttgccaatc ctcccggtct
     1861 ttcatctcga atgttcttcc tcggctgcct ctaccgtatc atcggttttt tgagatgcca
     1921 gtgtgtcgat tgctcatagg catgtgcggc ccgaagcacc gtctcttcct caaaagctcg
     1981 cccgatcagc tgtaagccaa tgggaagccc tgttttgctg aaaccgcacg gcagggcgat
     2041 cgccggcaat cctgccaggt tgaccgagat cgtaaaaata tcggagagat acatctgtaa
     2101 gggatcttcg ctcttttcac caagcttgaa ggcgggcgtc ggcgtggccg gagtcacgat
     2161 caaatccact tctttgaacg cggccgcgaa atcttggcag accaacgttc gcacagcttg
     2221 ggccttccca taataggcat cgtaataccc ggcgctgagc acatacgtcc ccagcatgat
     2281 ccgacgcttc acttccggtc caaacccttc ctgccgtgtc ttcatgtaga gttcgagaag
     2341 atctttggtt tccttggtac gaaaaccgaa cttcacgccg tcgaagcggg cgagattcga
     2401 gctggcttcc gccgtcgcga tcacatagta cacagccaca gccgcgccgg tgctcggcag
     2461 acggatttcc ctgatttcag cgccaagatt cttcaactcc ccaatggccg ctctgactgc
     2521 ctgttcgacg tccgagtcga gtccttcggt aaaaaattcg accggtacgc cgaccttcag
     2581 agttttgaga tcccgttttt gcagcgattt catgtaatcc ggcacggaac ggtcgaccga
     2641 ggtggaatcc atcggatcat gaccggcaat ggcctgcaga agaaatgccg cgtcaggcac
     2701 gtctctcgta atcggcccga tttgatccag cgaggaagcg aaggcaacca acccatatct
     2761 tgagacccgt ccgtaagtcg gcttcagtcc caccacgccg cagaaggctg ccggctgcct
     2821 gatggacccg cctgtgtctg agccaagtgc cgccacacat tcctccgccg ccaccgccgc
     2881 cgcagatcct ccgcttgatc ctcccggcac acagtgcaag ttccagggat ttcggctggg
     2941 accgaatgcg gagttttccg ttgacgatcc catcgcgaat tcatctaaat tcgtcttacc
     3001 caccaatagg tactcttgcg cacgcaactt ggcgacgacc gtcgcatcgt aaggcggtat
     3061 gaagttctgc aacattcgag agctgcagct cgtgtgaacc ccgttcgtac agatattatc
     3121 cttgatggca agaggcattc cggtaagcgg ggcgatcttt ctccaggcct tgagcttctg
     3181 gtccaaggcc tccgcctgta gggaggccga ctctttggct tgggtgacga aggccttcac
     3241 cttgggctct acttggctga t
//
LOCUS       DATMTI010000441         4661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_271034, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000441 DATMTI010000000
VERSION     DATMTI010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271034"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /gene="eno"
                     /locus_tag="VN039_07140"
     CDS             <1..624
                     /gene="eno"
                     /locus_tag="VN039_07140"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249434.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWV45778.1"
                     /translation="ISQAIEEASYKVGKDIALALDCAASEFYDKGRYVLEAEKNPERS
                     SDEMISYYGKLLDRYPILSIEDGLSELDWKGWKILTEKLGKRVQLVGDDIFVTNVEIF
                     SKGIKEGIGNSILIKLNQIGTLTETLDAIELAKRSGYTAIISHRSGETEDTTIADVAV
                     ATNSGLIKTGSLSRTDRVAKYNQLLRIEEELGAVALYRGREAVTSRA"
     gene            653..1012
                     /locus_tag="VN039_07145"
     CDS             653..1012
                     /locus_tag="VN039_07145"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWV45779.1"
                     /translation="MIIKQNRGRQWLKWRQRILIVMQAIGAGGCVWLFVALFSGEMGL
                     TRYLSMRDHASNLEQELSALRRENTILQRDITRLQHDPAKIEQLAREQLGYVRKGETV
                     YQLAPDPKKKPEPFSKP"
     gene            1009..1893
                     /gene="era"
                     /locus_tag="VN039_07150"
     CDS             1009..1893
                     /gene="era"
                     /locus_tag="VN039_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWV45780.1"
                     /translation="MKFGTVAIIGRSNVGKSTLLNRLLGEKISIVSSKPQTTRTRIMG
                     VAHVEGAQIAFLDTPGLHKPEHLLNRRMVRTAVETMEEADLFYMLMDATSLPGPGDLT
                     AVKHMKEALAKQPRPVILVVTKIDLVNKQKLLPILDRYGKLFAWTEVVPVSAQVDDNV
                     DRLLAVTVPYLPSGEGAYGDDVVTDQTIRTLAAEIIREKVLQETEEEVPYAVAVEIDE
                     FVEQGKLAKIRASILVERETQKGILIGKQGERLKAIGTQARLDMERLFDVKVFLELWV
                     KVKKAWREDEQTLVQLGY"
     gene            1904..3361
                     /gene="mgtE"
                     /locus_tag="VN039_07155"
     CDS             1904..3361
                     /gene="mgtE"
                     /locus_tag="VN039_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWV45781.1"
                     /translation="MQAERPIEPRVPDQRPRQSERDVLREILRDQTERGQTKSDIVIQ
                     SVQKLLRRGAITNLSKMLGRMHPADIAKVVTHLSSPKEKREIFELVRGEGKRGQALSE
                     LDGESIQQVLADLLHADIAWLLKDLGPDDVAYILGCLPEERSKEILALMKTEDSTEVA
                     DILKYPKDTAGAIMTTEFFSLPEDATAQEAIRRLQQATDAEMVFYIYVTDKDDRLVGV
                     LSLRQLITVPPTTPLKNIMTREVMSVAIDMDQEEVARQVASYNLLAIPVVERDGKLVG
                     IITVDDVVDVIREEATEDMLKMAGAIEEETGSKSSSFGSAKLRLPWLFTNLVGSLLSG
                     AILWYFRYTIQEVVAIVSFIPVIAAMGGNVGLQSSTLIIRGLAIGSVELTHVWPVFFR
                     EAKIGLVMGVACGVTLTLVAWVLHQGFLGMVVGASLIIAFLVSTSMATIMPILLKRVG
                     VDPAVAAGPFVTTANDITGISIYLTLATLFLEYLR"
     gene            3412..4170
                     /gene="recO"
                     /locus_tag="VN039_07160"
     CDS             3412..4170
                     /gene="recO"
                     /locus_tag="VN039_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWV45782.1"
                     /translation="MPLIKTTAIILRSRKWGEADRIVTLYSKDLGKIRGVARGARRQK
                     SRFGGAIEPFSVCCLNLFEKTGDSLFRISHVDLVRSSQVLREDLRLMASAARMVNVVA
                     AITPDGDPDPLLFDTLEQGLASLHESEDPTFMALLVQIRLLGLTGFRPQTDQCAACGK
                     RHFVGEPQFSPIAGGLVCLVCAARQRVRCVAFSRGSLLFLQQAIRLAPAVLTRLRAAG
                     QVRNEVEEAIEGYVTVVAGKRLPPVDFLSLALPG"
     gene            4226..4552
                     /locus_tag="VN039_07165"
     CDS             4226..4552
                     /locus_tag="VN039_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWV45783.1"
                     /translation="MEWLDKGVLGIQWSDGHKGIYPVRYLRQHCPCAACVDEWTGERR
                     LKSEDVPILIMLQDIESVGRYAFQFKWSDGHDTGIYSYSLLRKLCQCDICQPVKPVES
                     KSRRLM"
BASE COUNT         1062 a         1100 c         1437 g         1062 t
ORIGIN      
        1 atctctcagg cgattgagga ggcaagctat aaggtcggga aggatatcgc cttggcgttg
       61 gactgcgcgg cgagtgagtt ctatgacaag ggacggtatg ttcttgaagc ggaaaagaat
      121 ccggaacgtt cgtcggatga gatgatcagc tactatggaa agttgctgga tcgttatccg
      181 atcctctcga ttgaggacgg gttgagcgaa ttggattgga aggggtggaa gattctcacg
      241 gagaagctgg gcaaaagggt gcagcttgtc ggcgacgaca tcttcgtcac caacgtggaa
      301 atcttttcaa agggcatcaa ggaaggaatc ggaaattcaa ttctgatcaa gctcaatcag
      361 atcgggaccc tgacggaaac cttggatgcg atcgaacttg cgaagcgatc gggttatacg
      421 gcgatcattt cgcaccggtc cggtgagact gaagatacca cgatcgcgga cgtggcggtc
      481 gcaacgaaca gtggattgat caagacgggg tccttgtctc gaacggatcg cgtggcgaaa
      541 tataaccaat tgctccgaat cgaagaagag ctgggagccg tggcgcttta tcgtggtcga
      601 gaggcagtga cgtccagggc ctagctggta cgtagtcggt ggggtggaga gaatgattat
      661 caagcaaaat cgtggacggc agtggctgaa atggcgacag cgcatactga tcgtcatgca
      721 ggcgatcggc gctggcggct gtgtgtggct gttcgtggcc ttgttttccg gagaaatggg
      781 cctgactcgg tatctctcga tgcgtgatca cgccagcaat ttagagcaag aactctcggc
      841 tttacgtcga gaaaatacca tattgcagcg agatattacc cgtctccagc atgaccccgc
      901 caagatcgag cagttggcac gagaacagtt gggctatgtt cgcaagggcg aaaccgtgta
      961 tcaattagcg ccggatccta agaaaaaacc ggagcccttc tcgaaaccat gaagttcgga
     1021 acggtggcca tcattggccg gtccaacgtc ggcaagtcga cgctgctcaa tcgcctgctt
     1081 ggcgaaaaaa tttcgattgt gtcgagtaag ccgcagacca caaggacccg catcatggga
     1141 gtcgcgcatg tggaaggagc acaaattgcc tttctcgaca caccggggct tcataaaccc
     1201 gagcacctgc tgaatcgtcg aatggtgcgc actgccgtgg agaccatgga agaggcggat
     1261 ctgttctaca tgctcatgga tgcgacgagt ctgccgggtc ccggagatct gaccgcagtg
     1321 aagcatatga aggaggcgtt ggccaagcag ccgcgcccgg tcattcttgt cgtcacgaaa
     1381 atcgatctcg tcaacaagca gaagttgctc ccgattcttg atcgttacgg aaaactcttt
     1441 gcgtggacgg aggtcgtgcc ggtttccgcc caggttgacg acaatgtgga ccgattattg
     1501 gccgtgacgg ttccttatct tccgtccgga gaaggagcct acggcgacga tgttgtgaca
     1561 gatcagacaa tcagaacgct ggcggctgag ataattcgtg agaaggtgtt gcaagagacg
     1621 gaggaagaag tgccgtatgc ggtcgcggtc gaaatcgatg agttcgtcga gcagggcaaa
     1681 ttggcgaaga tccgggcgtc gattttggtg gagcgagaaa cgcagaaggg catcctcata
     1741 ggtaagcaag gagagcgttt gaaagcgatc ggtacgcaag ccagactgga tatggaacgg
     1801 ctattcgacg tgaaagtgtt ccttgaactg tgggtaaagg tgaagaaggc gtggcgtgag
     1861 gacgagcaga ccttggtgca gttggggtat tgacggaacg gatatgcagg cggaacgacc
     1921 gatagagcca cgggtaccgg accagcgacc tcgccagtcc gaacgcgacg tgttgcggga
     1981 aatcttgcgc gatcagaccg aacgcggtca gacgaagtcg gacattgtaa tccagtcggt
     2041 gcagaagtta ctgcgacgcg gagccatcac caacctctct aagatgttgg ggcgtatgca
     2101 tcccgcggac atcgccaaag tcgtgaccca tctctcttct ccgaaggaaa agcgtgaaat
     2161 tttcgagttg gtccgcggcg aaggcaagcg cggacaagcg ctcagtgaac tcgacggcga
     2221 aagtattcaa caagtactcg ccgatctgtt gcatgccgac atcgcgtggc tcttgaaaga
     2281 tctcggtccc gatgatgtgg cgtacatcct cggatgcctt cccgaagagc gcagcaaaga
     2341 aattctcgct ttgatgaaaa ccgaggattc gaccgaggtt gccgatatct tgaagtaccc
     2401 gaaggatacg gcaggcgcga ttatgaccac ggagttcttt tccttgccgg aggatgccac
     2461 ggcacaggaa gcgattcgtc gattacagca agccaccgat gcggaaatgg tgttctacat
     2521 ttatgtaacg gacaaggatg atcgcctggt cggtgtcctc tcgcttcggc agctgatcac
     2581 cgtccctccg acgactccgc tgaaaaatat catgacccgg gaggtcatga gtgtggcgat
     2641 cgacatggat caagaagagg tcgcgcgcca ggtggccagc tataatttgc tggctattcc
     2701 ggttgtggaa cgggacggaa aactcgtggg catcatcacg gttgatgacg tagtcgatgt
     2761 gatcagggaa gaagcgaccg aagacatgtt gaagatggcc ggggcaattg aagaagaaac
     2821 gggctcgaag tcttcgagct tcggctcggc aaaactgcgg ttgccatggc tcttcaccaa
     2881 cctagtcggc agtctcctgt ccggtgcgat cctttggtat ttccgttata cgattcagga
     2941 agttgtggcg atcgtgagtt ttattcctgt gattgcggcg atgggtggca atgtggggct
     3001 acaatcctcg acgttgatta ttcgagggtt ggccatcggc tctgtggaac tcacccatgt
     3061 atggcctgtc ttttttcgtg aagcgaagat cggcttggtt atgggggtgg cctgcggtgt
     3121 gacgctgaca ctggtcgcat gggttttaca ccaagggttt ttgggcatgg tcgtcggagc
     3181 ttcgctgatc attgcctttc tggtgtcgac cagcatggcg acgattatgc cgattcttct
     3241 caaacgggtg ggggtcgatc cggccgttgc ggctggtcct tttgtcacga cggctaatga
     3301 catcaccggt atttcaatct acctgacttt ggccactctt ttcttggagt atctccgatg
     3361 atacggacag gtacgtcgcc acgcttccgt cgcggtcggg agtagagagc cgtgccgctg
     3421 ataaagacga cggcgatcat tttgcggagc cgcaagtggg gcgaagccga tcgaatcgtc
     3481 acgttgtatt ccaaagatct tggtaaaatc cgaggcgtag ctcgtggtgc ccgtcgtcag
     3541 aaaagccgct tcggaggtgc aatcgaaccg ttcagcgtgt gttgcctgaa cctatttgaa
     3601 aaaacagggg attcactgtt tcgtatttcg catgttgatc tcgtgcgttc gtctcaagtg
     3661 ctgcgggaag accttcgttt gatggcatcg gcggcgcgaa tggtcaatgt cgtagcagcg
     3721 atcactcctg atggagatcc ggacccgctc ttgtttgata ccctggagca aggcctcgcc
     3781 tcgctccacg aaagtgaaga cccgaccttt atggcgctcc ttgttcagat cagactgctg
     3841 ggattgacag ggtttcgtcc gcagacggat cagtgcgccg cctgtgggaa gaggcatttc
     3901 gtcggggagc ctcaattttc tccgatcgcc ggaggcctcg tgtgcctggt ctgtgccgca
     3961 cgtcaacgag ttcgatgtgt cgcattctca cggggcagcc ttttgttcct ccaacaagcc
     4021 attcggctgg ctccggcagt actcacacga ttgcgagctg cggggcaagt acggaatgag
     4081 gtggaagaag cgattgaagg atacgtaacg gttgttgcag gaaagcggct accgccggtt
     4141 gacttcctat cgctcgcatt gccaggatga ggcagacacg gtgagaggtg aaaacatggc
     4201 agccagcgct ttggagcctc gagatatgga gtggcttgac aagggagtgc tgggcatcca
     4261 gtggagcgat ggccacaagg gtatctatcc agttcgttat ctgcggcagc actgtccctg
     4321 cgcggcttgt gtcgatgagt ggactggcga gcggcgtctt aagtcagagg atgtgccgat
     4381 cttgattatg ctccaggata tcgagtcggt gggacgctac gcgtttcagt ttaaatggag
     4441 cgacggccac gatactggta tctattctta ttcactgcta agaaaattgt gtcagtgcga
     4501 catctgtcag ccggtgaaac cagttgagtc aaagtctcga cggctcatgt gaggctagga
     4561 tacgacgtga ccaatatttc tgttgccgcg ataaaggcgc tctggtgttt aagtggactt
     4621 ttggcattcg tattgagcgt aggttgcaag agcatgccga c
//
LOCUS       DATMTI010000442         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_526521, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000442 DATMTI010000000
VERSION     DATMTI010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526521"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..638)
                     /locus_tag="VN039_07170"
     CDS             complement(117..638)
                     /locus_tag="VN039_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWV45784.1"
                     /translation="MTRRMNASLVILLVLSVSIGVFAFARDRSLPLQGLYASGRLFES
                     VWVELLLGFLIAGFIDVLISPAQITAWMGTESSTRGIFVGWMAGLVIPGGPYLLFPLA
                     ASLWKTGVPPGALIALITAKTLVSPIRILTYEAPLLGWRLTIARCVPALLVPPIMGIL
                     GQWIFNLFVRKSS"
     gene            complement(635..1729)
                     /gene="pqqE"
                     /locus_tag="VN039_07175"
     CDS             complement(635..1729)
                     /gene="pqqE"
                     /locus_tag="VN039_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HWV45785.1"
                     /translation="MHRPYTLIAELTYRCPLRCPYCSNPWDLAEHGNEIDTDTWLRIF
                     HEAEELGVVQLNLTGGEPLLRDDLELLIEGAGKLDLYTNLITSGMPLTSERLSRLRTL
                     GLDSVQISIQSTRSSLSDRIAGAPSFSRKLAAMQWVTSLGLPLTMNAVLHRENISEIQ
                     EIIALAECVSADRLELANTQYLGWALKNRAALLPTREQIERARTVAAEAKARLRGKME
                     VLFVTPDYYTEYPKSCMEGWGRRFIVVNPEGLALPCHLAHTIPGLYFEHVTQRRLADI
                     WHHSAGFNRFRGEDWLPDPCRTCERRAVDFGGCRCQAFHVVGNAGMTDPACSLAPGHN
                     HIESARAQADRTTGFPVLFEYRTGRGCSSR"
     gene            complement(1735..2016)
                     /gene="pqqD"
                     /locus_tag="VN039_07180"
     CDS             complement(1735..2016)
                     /gene="pqqD"
                     /locus_tag="VN039_07180"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HWV45786.1"
                     /translation="MILTTVRPRLSPKVRLRLDRSTGRHLLLYPERGLELNDTATVIA
                     RLCTGEWTIDEMVDHLTRAYDDVSSNEIRRGVCEFLDVLVDRGLLHTMS"
     gene            complement(1985..2752)
                     /gene="pqqC"
                     /locus_tag="VN039_07185"
     CDS             complement(1985..2752)
                     /gene="pqqC"
                     /locus_tag="VN039_07185"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HWV45787.1"
                     /translation="MMKEDHHRDRLSQDAFIEWLKREGSRRYHDRHPFHERMHNGNLT
                     KTQLQQWVLNRYYYQTRIPIKDALIVSKSEDPTFRRMWLRRVRDHDGEQEGEGGLALW
                     LELARGVGLDAEEVRSLRYVLPGVRLACDDYVRFVREAPLLDAVASSLTELFAPSLMA
                     RRLEAWKQHYPWVCSESLEYFQVRISRAVLDSNQAIEFVVRHATTYSDQARCVRALVK
                     KADILWTMLDHLSMAYVETGTEGKQTAHDSHHSQTSA"
     gene            complement(2752..3675)
                     /gene="pqqB"
                     /locus_tag="VN039_07190"
     CDS             complement(2752..3675)
                     /gene="pqqB"
                     /locus_tag="VN039_07190"
                     /inference="COORDINATES: protein motif:HMM:NF003523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HWV45788.1"
                     /translation="MLIRVLGSAAGGGFPQWNCACVNCKGMRKGLIAASPRTQESISF
                     STGDGNWFLINASPDIRAQIESFGSLHPRQSRSTPIQAVFLTNGDLDHCLGLLSLREN
                     HRLVLYATDSVRRGFTEGNVLYRTLQRFAGQVTWRTLKLDVEEPVLLADGRPSGLTVT
                     AVAVPGKPPIHLEGLVSSSETDVNVGLRFRQSINGGVLAYFPAVGRITSSVPNALEGA
                     GCVMFDGTFWSSDELSVPGFLAKSAEDLAHCPVGGSEGSLAMLAKIAASRRVFIHINN
                     TNPMLREDSHERKLVEAAGWEIAWDGMEILL"
BASE COUNT          878 a         1083 c         1058 g          780 t
ORIGIN      
        1 atcgagagga agagaggcgg cttcgatccg gttcccgaag aagaacagca tcagcgaagc
       61 gcaaaccggt ccgacaatgc gattcatgac tgtgcttctt cttacgccgt ttatgttcat
      121 gatgacttcc tcacgaatag attaaagatc cactgtccca gtatccccat aatcggagga
      181 accaagagag caggcacaca ccgagcaatg gtcaaccgcc accccagcag aggagcttca
      241 taggtcagga ttctaatggg gctgaccaaa gttttggcgg taatcagagc gatcaaggcg
      301 ccgggaggca ccccggtttt ccagaggctt gctgccaggg gaaacaagag atagggtcct
      361 ccaggaatca ctaagccggc catccacccc acaaaaattc cgcgtgtcga ggattcggtt
      421 cccatccacg cggtgatttg agccggggaa ataagcacat cgataaaccc ggcaataaga
      481 aagcccaaca gcaattcgac ccagacgctc tcgaatagcc ggccgctcgc atagagcccc
      541 tgcagcggca acgagcgatc tcttgcaaat gcgaacacac cgatagacac ggacagcaca
      601 agcaaaatga ctagactggc attcatgcgt cgggtcatcg cgaagagcac cctcgaccgg
      661 tccgatattc gaacaggacg gggaagccgg tcgtgcgatc ggcctgagct cttgccgact
      721 caatgtggtt atggccggga gcgagagagc aggcggggtc ggtcatgccg gcatttccca
      781 cgacatggaa agcttgacag cgacagcccc cgaagtctac agcgcggcgc tcacaggttc
      841 tacaaggatc gggcagccaa tcttcgccac ggaatcggtt gaatcctgct gaatgatgcc
      901 agatatccgc gagccggcgc tgcgtgacat gctcgaaata caatcccggt atcgtatgcg
      961 ccagatggca gggcaaggcg agcccttccg gattgaccac gatgaatcgg cgcccccatc
     1021 cttccatgca ggatttgggg tactcggtgt aatagtcggg cgtgacgaag agcacctcca
     1081 tttttccacg aagacgtgct ttggcctcag ccgcaacggt tcgcgcccgc tcgatttgct
     1141 cccgagtggg taggagcgcg gcgcgattct tcaatgccca tccaagatac tgcgtattcg
     1201 ccaactcgag ccgatcggcg gaaacacact cagccagagc gatgatttct tggatttcag
     1261 agatattctc tcgatggagc acggcgttca tggttaatgg aagcccgagt gatgtgaccc
     1321 actgcatggc ggcgagcttt cggctgaacg atggtgctcc ggcgattcgg tccgataggg
     1381 acgatctcgt actttggatg gatatctgca cgctatccag tcccagtgtg cgaagtcgag
     1441 agagccgctc ggacgtgagc ggcataccgc tcgtgatgag gttcgtatac aggtcgagct
     1501 tgccggcacc ctcgataagc agttccagat catctctcaa caacggctcg cctccggtga
     1561 ggttgagttg aaccacgccg agttcttcgg cctcatgaaa gatccgaagc catgtgtcgg
     1621 tgtcgatttc gttcccatgt tccgccaggt cccacggatt cgaacagtag ggacagcgaa
     1681 gcggacaccg ataggtgagt tcggcaatca atgtatacgg acgatgcacg ttgttcatga
     1741 catagtgtga agcaggccgc gatcgacaag gacatccaga aactcgcaca cccctcgcct
     1801 gatctcgtta gaagacacgt catcataggc tcgggtgaga tgatccacca tctcatcgat
     1861 cgtccattca cccgtgcaca gacgcgcaat caccgtggcc gtgtcgttca gttcgagtcc
     1921 cctttcgggg tacaagagca ggtggcgacc ggtactccgg tcgagccgaa ggcgaacctt
     1981 gggactaagc cgaggtctga ctgtggtgag aatcatgagc agtttgcttt ccttctgttc
     2041 ccgtttcgac atatgccata gagaggtgat cgagcatggt ccacaaaatg tcggccttct
     2101 tgaccaaggc cctcacacat cttgcttggt cggaataggt ggtcgcgtgc cgaaccacga
     2161 attctatggc ctggttcgaa tcgagaacgg cacgagaaat acgcacctga aaatactcca
     2221 gggactccga gcacacccag ggataatgtt gtttccacgc ttcgaggcgc cgcgccataa
     2281 gggaaggcgc aaacaattcc gtgagcgagg aggccacggc atcgagaagc ggagcctctc
     2341 ggacgaaccg gacatagtcg tcgcacgcga gcctgactcc cgggagtacg taccggagac
     2401 tcctcacttc ctctgcgtcg agaccgacgc ctcgcgccaa ctcaagccat agggcgagtc
     2461 cgccttctcc ttcctgttcg ccgtcatggt cccggacgcg acggagccac atccgccgaa
     2521 acgtgggatc ctcggatttc gaaacaatga gggcatcctt gatgggaatt cgcgtttgat
     2581 agtagtagcg gttcaatacc cactgttgga gctgtgtctt cgtcaaattt ccgttgtgca
     2641 tacgctcatg gaaaggatgg cggtcatgat agcgacgaga accttcccgc ttgagccatt
     2701 cgataaaggc atcctgtgag agccgatccc gatggtgatc ctccttcatc atcacaacag
     2761 gatctccatt ccgtcccatg cgatctccca tcctgccgcc tcgacaagtt ttcgttcgtg
     2821 agaatcctct cgtaacatcg gattggtgtt gttgatgtgg ataaagaccc ggcgagacgc
     2881 ggcaatcttc gcaagcattg caaggcttcc ctcggatcca ccgacagggc aatgcgcgag
     2941 atcttccgcc gattttgcaa ggaagcccgg gacggacagt tcatcgctcg accaaaacgt
     3001 cccgtcgaac atgacgcaac cggctccttc gagcgcgttg ggaactgagg acgtaatccg
     3061 gccgacggcg gggaaatagg ccagcactcc gccgttgatc gattggcgaa atcgaagtcc
     3121 cacattcacg tccgtttcac tggatgaaac gagtccttcc aaatggatcg gaggcttgcc
     3181 tggaacggcc acggctgtca ccgtcagtcc cgacggcctt ccatccgcaa gcaacaccgg
     3241 ttcctcaaca tcgagtttca gtgtccgcca tgtgacctgt ccggcgaaac gctgcagtgt
     3301 ccgatacaag acgttgcctt cggtgaatcc gcgacgcacg gagtcggttg catacaggac
     3361 gagccggtga ttctcccgga gcgagaggag ccccaagcag tggtccagat ctccattggt
     3421 caaaaagacg gcttggatcg gtgtagaacg cgattgacgg ggatggaggg agccaaagct
     3481 ttcgatctga gcacgaatgt ccggagaggc gttgatgaga aaccagttac catcacccgt
     3541 gctgaaactg atggattcct gggtccgagg gctcgcggcg atcagccctt tccgcatgcc
     3601 tttgcaattc acacaggcac agttccactg tgggaatcca cctcctgcag ccgaccctag
     3661 aacccgaatg agcatcacat ccatccattg ctattcttga cgagtggtga taaccggtgt
     3721 ctccgtcgat gggtccttcg cgtcgggacc gtctcgaaaa tcatcctggt acgagttgat
     3781 ctcggcgtcc attttgatt
//
LOCUS       DATMTI010000443         1735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_101651, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000443 DATMTI010000000
VERSION     DATMTI010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101651"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VN039_07195"
     CDS             complement(<1..184)
                     /locus_tag="VN039_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45789.1"
                     /translation="MSETLSEHAAQALEWPRLLEILALHAQSGMGAAQCRSISLSSDL
                     ADARLRQQETTEMVSVL"
     gene            330..1343
                     /gene="gap"
                     /locus_tag="VN039_07200"
     CDS             330..1343
                     /gene="gap"
                     /locus_tag="VN039_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWV45790.1"
                     /translation="MAIRVGINGFGRIGRNVLRASMGDKDLQIVAINDLTDAKTLAYL
                     LKYDSVHGTLPANVEAKEDQILVDGNPIKVLAIKDPKELPWKALNVDVVIESTGRFTD
                     REAAGKHLSAGAKHVIISAPAKDPDVTIVLGVNEDKFDPKSHHIVSNASCTTNCLAPV
                     AKVLLETFGIKHGIMTTIHSYTNDQQLLDLPHKDLRRARAAGVSMIPTSTGAAKALHL
                     VIPELKGKLDGLAIRVPTPNVSLVDLTVETEKDCDIASVNAAFKKAADGPLKGILKYS
                     EDPIVSIDQKGDPHSATVDAPLTNVVDKRLVKVTAWYDNEWGYSCRVRDLVKVLAGKS
                     TTH"
     gene            1440..>1735
                     /gene="pgk"
                     /locus_tag="VN039_07205"
     CDS             1440..>1735
                     /gene="pgk"
                     /locus_tag="VN039_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWV45791.1"
                     /translation="MHKKTIDDVQLRGKRVIIRADFNVPLDESLQITDDTRIRSTLPT
                     INRVVDEGAKVILCSHLGRPKGTFDPKYSLAPVAKRLGRLLGKDVVFAPDCIGPA"
BASE COUNT          419 a          491 c          448 g          377 t
ORIGIN      
        1 ccagcacact caccatctcg gtcgtctctt gttgacgaag gcgggcatcc gcaaggtcgc
       61 tcgacaagga aatcgatcgg cattgagcag ctcccatccc cgattgtgca tgcagtgcga
      121 gaatttccaa taaccggggc cactccaacg cctgtgccgc atgttcagat aatgtctcgc
      181 tcacatgagc ctcctaaccc gtctgacttc attttggacg ctagccaagt gtcttgacaa
      241 ggaggagagg gcatcgatac tatcgctgtt cgtgctgtgc cattcgagtg tattcaacca
      301 aaatatattt gcatgtagag gatcacgcta tggccattcg tgttggaatc aatggattcg
      361 gacgtatcgg gcgcaacgtg ttgcgcgctt cgatgggtga caaagatctc caaatcgtcg
      421 ccatcaacga tctcacggac gcgaagaccc tcgcttacct gctcaagtat gactcggtac
      481 acggcacgct cccggccaac gtcgaagcca aagaagacca gattctggtt gatgggaacc
      541 ccatcaaggt gctcgcgatc aaagatccaa aggaattgcc ttggaaggct ctgaacgtcg
      601 atgtagtgat tgaatcgacg ggccgattta ccgatcggga agcggcaggc aagcatctgt
      661 ctgcaggcgc caagcatgtg attatttccg caccggcgaa agatcccgat gtgaccattg
      721 tcctgggtgt gaatgaggat aagttcgatc cgaaatcgca tcatattgtg tccaatgcct
      781 cttgcacgac caactgtctg gcgcctgtcg ccaaagtctt gttggaaacg ttcggtatca
      841 agcatgggat catgaccacg atccattcct ataccaacga tcagcagctg ctcgaccttc
      901 ctcacaaaga ccttcgacgc gcccgcgcag ccggcgtgtc gatgattccc acgagcaccg
      961 gcgcggccaa agcgctgcat ctcgtcattc ctgaactcaa gggcaagttg gatgggcttg
     1021 ccattcgggt gccgacaccg aacgtatcgc tggtggatct caccgtcgaa accgagaaag
     1081 attgtgacat agcatccgtc aacgccgcct tcaagaaggc ggcggatggc cccctgaagg
     1141 gcattctcaa gtactctgaa gaccccatcg tctcgatcga tcaaaaaggg gaccctcact
     1201 cggccacggt cgatgccccg ctgaccaacg tcgtggataa gcgtctcgtc aaagtcacgg
     1261 cttggtacga caatgaatgg ggctattcat gccgagtccg cgacctggtc aaggttctgg
     1321 ccggaaaatc caccactcac tgatgaccac gtcgaagatg atcgggcagg tttcagacaa
     1381 acccatctca atcgctttat ttcacaggtc ataggatact ggaggaacgc ctcatgaatt
     1441 tgcacaagaa aacgattgat gatgtgcaac ttcgtggtaa gcgggtcatc atccgcgccg
     1501 atttcaacgt tccgctcgac gaatcgctgc aaattaccga cgacacccgt attcgctcaa
     1561 ccctgccgac gatcaatcgc gtcgtcgacg aaggagcgaa agtcattctc tgctctcacc
     1621 tcggtcggcc caagggaacc ttcgatccaa aatatagcct ggcacctgtt gcaaaacgac
     1681 tgggacgatt gctcgggaaa gacgtggtct tcgctccgga ctgtataggt ccggc
//
LOCUS       DATMTI010000444         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_144490, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000444 DATMTI010000000
VERSION     DATMTI010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144490"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VN039_07210"
     CDS             <1..624
                     /locus_tag="VN039_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45792.1"
                     /translation="TSGLIAPGTVITAYGTGIGGLGTYLVNNSQAVASEAMTSSAQPL
                     YGYCNAWALPSDFRRFVRPHKRPQNRFAYSWLWSPEGWGWYSRSDPPFWPCGWPYVVE
                     TLAGDGNKYVLTDYNGSLGSAMINYIRLITDYTQLMPGFVNCLANRIAMELCIGITED
                     KSKFQLMEERYKESLNSAEAQNETMDFQHDEAGSESWERAGRFVRGW"
     gene            625..>3186
                     /locus_tag="VN039_07215"
     CDS             625..>3186
                     /locus_tag="VN039_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45793.1"
                     /translation="MPKTYPVRNSFNAGEVSDLVSFRDDVTKYHSACLTLENALPLVE
                     GGAKKMPGTYFAGAAGSSGATFTGSITGNALSVTAVAAGTIRIGQTITGQGVAPGTVI
                     TGFTPTSTPFFTYYNVSNNAVGLHGSGDWQYVGNQFRTISQPVTTNLADLNFSNFGFN
                     IPAGATILGVTVSAVLVSQFTTSSVLSQVSLWNSSGLLGSIKTPNTPFVPSLQTEAYG
                     NSTDLWGATLTPAIVNDPSFGFAMAVNTPDTTRVFIGQAFQMTVYYEIETIIVSGQPG
                     GVGNYTVSVSQSVTSETMYTESNGKSRLVPFQFSTEQGAVLEFSAGIVRIWEGATAGS
                     WSLGLALQTPPTGANYNPATAYVAGNIALVGPYAPALFYTSTSSGWVPNPSLGVLTFA
                     APYGTSFANIVPITFTVNSTDALSVTVTGSSPNQGINIALAKTTRALNAATAIQAAIR
                     ALGTLNAPGNNYVDLSGWTVTPDPLYFATPWITAPTTAPGSQVSVGFTASFIAQCVSA
                     NSGDQFPVIYTGAFDATYWAVYNATAQLPIELTTPYTEADLFSLDCSTQSADVLWIFH
                     SNYPPAVIERLGANSWVYSPSLPGQQPGQPAYRGTLGVVKTGYSALGQSITGITKANP
                     CVVTVAATNTAFLDGDRVYINLVSGMVELNQGEFIVANSTSGSGTFSFSLIDPNTGLA
                     VDSTGYLAYVSGGFAVKVFPLFSAPGDYPACGALYQERLCVGGSDNNPTQINGSVEGD
                     YPDFICDPNQDDFAIQFTLVSKQVNRLLNMIGTPNALLIGTSGGVWVVEGSNGLSLSQ
                     TNVTASLQTSLGVSALQPQMVNGSAIFVSRSARIVTFLVFNFASNQWEANDLTRLN"
BASE COUNT          649 a         1090 c          799 g          648 t
ORIGIN      
        1 acctccggcc tgattgcgcc cggtacggtc atcacagcct acggaacggg cattggtggt
       61 ctcggaacct atctcgtcaa caattcccaa gccgtagcga gcgaagccat gacctcttcc
      121 gcccaacccc tctatggcta ctgcaacgcc tgggcactcc cctccgactt ccgccgcttc
      181 gttcgcccgc acaagcgccc gcagaataga ttcgcctact catggctgtg gtcgccggaa
      241 ggctggggat ggtatagccg aagcgacccc ccattctggc cttgcggttg gccctatgtg
      301 gtcgagacct tagccggaga cggtaacaaa tacgtcctca ccgattacaa cggttcgctc
      361 gggtcggcga tgattaacta catccgcctt atcaccgact acacccagtt gatgcccgga
      421 ttcgtcaact gcctcgccaa ccggattgcg atggagttgt gcatcggcat caccgaggac
      481 aagagcaaat ttcaactgat ggaggagcgt tataaggaat cgctcaactc tgccgaagcc
      541 caaaacgaga cgatggactt tcagcacgat gaggccgggt cggaatcgtg ggagcgtgcc
      601 ggtcgattcg tgaggggatg gtagatgccc aagacctatc ccgtccgcaa ttcgttcaac
      661 gccggtgagg ttagcgatct cgtatccttc cgcgacgacg ttaccaaata ccacagcgcc
      721 tgcttgacac tcgaaaacgc ccttcccttg gtcgaaggcg gcgcgaaaaa gatgccggga
      781 acgtacttcg ccggggctgc ggggtcgagc ggtgcgacct tcactggatc aatcaccggc
      841 aatgctctct cagtcacagc ggtcgccgcc ggaactatcc gcatcgggca gaccatcacc
      901 ggccaaggtg ttgcccctgg caccgtcatc accggcttta ccccaaccag caccccattc
      961 ttcacttact acaacgtctc gaacaacgcc gtgggactgc acggctccgg tgactggcaa
     1021 tatgtcggga atcagttccg cactatctcc caaccggtca cgaccaacct tgccgacctg
     1081 aacttctcca acttcggctt caacattcct gccggagcga ccatcctagg cgtcaccgtc
     1141 agcgctgttt tggtgtccca gttcaccaca tccagcgtgc tctctcaggt ctcgttgtgg
     1201 aactcttcag gactgcttgg gtcgatcaaa acccccaaca ctccctttgt tccttcgctc
     1261 caaactgaag cctacggaaa ttccaccgac ctttggggcg ctaccctaac ccccgccatc
     1321 gtcaatgacc ccagtttcgg gtttgcaatg gccgtcaaca ctcccgacac gacgcgagtg
     1381 ttcatcgggc aagcatttca gatgacggtg tactacgaaa tcgaaacgat catcgtcagc
     1441 ggccagcctg gcggcgttgg caactacacg gtcagcgttt cgcagtcggt gacgagcgag
     1501 acgatgtaca ccgagtcaaa cgggaagagt cgccttgtgc cgtttcagtt ctcgaccgag
     1561 caaggagccg tcttagaatt ctctgctggt atcgtccgta tctgggaagg ggcgacagca
     1621 ggatcgtggt ctcttggact agccctccaa acccctccaa ccggagccaa ttacaacccc
     1681 gcgaccgcct acgtcgccgg aaacatcgcg ctcgtcggcc cttacgctcc ggcgcttttc
     1741 tacacctcga ccagctccgg gtgggttccc aacccatccc tcggcgttct cacctttgcc
     1801 gcgccttacg ggacgagctt cgccaacatc gttcccatca ccttcaccgt caacagcacc
     1861 gacgcgctca gcgtgacggt aacgggaagc tcgccgaatc agggcatcaa catcgcgctg
     1921 gccaagacta ctcgtgccct caacgctgcc actgctatcc aagcagccat ccgtgcgcta
     1981 ggcacgctca acgcccccgg caataattac gttgacctga gtggatggac ggtcacgcct
     2041 gaccccctat acttcgcaac gccgtggatt accgcaccca ccactgcacc ggggtctcag
     2101 gttagtgtcg gattcaccgc ctcgttcatc gcgcagtgcg tttcggctaa ttccggcgac
     2161 caattcccag tcatctacac cggcgcgttc gacgcgacct actgggcagt ctataacgcc
     2221 acggcacaac ttccgattga actcaccacc ccttacaccg aagctgacct cttctcgctc
     2281 gactgctcga cccaaagcgc ggacgtgttg tggatttttc attccaacta ccccccggcg
     2341 gtcattgaac gcctcggggc gaactcgtgg gtttacagcc cttcccttcc cggccagcaa
     2401 cccggacagc ccgcctatcg cggaacgctg ggcgtggtca agacaggata ctccgctctc
     2461 ggccaatcca tcaccggaat caccaaagcc aacccctgtg tagtcaccgt agccgcgacc
     2521 aacaccgctt tcctcgacgg cgaccgtgtt tacatcaacc tcgtctctgg gatggtagag
     2581 ttgaaccaag gcgagttcat cgtggccaac tctacctccg gttcaggcac gttctccttc
     2641 agcctcatcg acccgaacac cggcctcgcc gtagactcca caggctatct cgcctacgtc
     2701 agtggcggat ttgcggtcaa ggtcttcccg ctattctccg cccccggcga ctaccccgcg
     2761 tgcggcgcgc tctaccagga acgcctctgc gttggcggca gcgacaacaa cccgacccag
     2821 atcaacggct cggtcgaggg cgactacccg gacttcatct gcgaccccaa tcaggacgat
     2881 ttcgccatcc aattcaccct cgtctcaaag caggtcaacc gcttgttgaa tatgatcggc
     2941 acccccaacg cgctgctaat cggcacctcc ggcggtgtgt gggttgtgga gggaagcaat
     3001 ggcctctctc tgagccaaac caacgtcacc gcgtcccttc aaaccagcct tggggtcagc
     3061 gcccttcagc cgcagatggt aaacgggtcg gcgatcttcg tctctcgttc cgcgcgcatc
     3121 gttactttcc tcgtcttcaa cttcgcgtcg aaccagtggg aagcgaacga cctcacccgg
     3181 ctcaac
//
LOCUS       DATMTI010000445         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_187510, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000445 DATMTI010000000
VERSION     DATMTI010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187510"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1033)
                     /locus_tag="VN039_07220"
     CDS             complement(101..1033)
                     /locus_tag="VN039_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45794.1"
                     /translation="MVVRAKQVWRRLGRVIEPQPAGGWWLSHASYPTALVQKDGHVTV
                     FFSVRDEANKSSLASVELEIYGDRFVAGPVRGPLFTPGPRGAFDADGVTVTSFVRQGA
                     RLLAYYLGWTKGVSVPFTNFIGLAEAQPDGTFKRLSPAPIVGRSPENPFTLGYPWVVK
                     TSGELRMWFGSHLFWGDSGLEMNHVIKQASSRDGVLWTQMPGVAVPLAGEADVAEFAV
                     SRPVVIEEPDDSLSMWYARRNPQYSIGYAVSIDRGLSWQRKDELFTFEGEPEAWELQE
                     TTYPCVFDFNGSRYMLYNGDGYGRTGFGLAKLEI"
     gene            complement(1036..>1531)
                     /locus_tag="VN039_07225"
     CDS             complement(1036..>1531)
                     /locus_tag="VN039_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HWV45795.1"
                     /translation="QFPPAEYDAFVALSYNRMNVLRAQRVDAMRALGYTMTNYISPNA
                     TVLTDKIGDNCLIFEDNTLQPFCRIGNNVTLWSGNHIGHHSVIEDNVFISSHVVISGG
                     VTVGHNSFIGVNSTIVDHIEIAPFTLLGAGCLVQQSTEREGVYAPTSTIEKRKVPSTR
                     LRNI"
BASE COUNT          379 a          440 c          384 g          328 t
ORIGIN      
        1 acctatgact tattcgccgc ttgaggcgtg gctcacggct tccgcaaaag atagtgctgg
       61 tggcccttct atctggccgt ttgcccggcg gggcgcgaac tcagatttcg agtttggcca
      121 agccaaatcc cgttcggccg taaccatccc cattataaag catgtatcgg cttccattga
      181 aatcaaacac gcatggataa gtggtttcct ggagctccca agcctctggc tcaccctcaa
      241 acgtaaacag ctcgtccttg cgctgccaac tcaatccccg gtcaatggag acagcataac
      301 cgatagaata ttgtggatta cggcgtgcgt accacatgga cagactgtca tccggttcct
      361 cgatgaccac cgggcgggaa acggcaaatt cggcaacatc cgcttctccc gccaagggca
      421 cggctacgcc aggcatctga gtccaaagca cgccatctct tgaacttgcc tgctttatga
      481 cgtggttcat ttccaggccg ctatcgcccc agaacagatg gcttccgaac cacatacgaa
      541 gttccccgga agttttcacc acccatgggt agcccagagt aaaaggattc tcagggcttc
      601 ttccaacaat cggagcagga ctcaatcgct tgaatgtccc gtcgggttga gcttcagcaa
      661 ggcctataaa attagtaaaa ggaaccgaca ctccttttgt ccagccaaga taataggcca
      721 acaatctcgc gccctgccga acaaaagatg tcaccgtgac gccatcagca tcgaacgcgc
      781 cgcgtgggcc gggcgtaaac aaagggccgc ggacaggacc agccacgaag cgatccccat
      841 atatctcgag ttcgacggat gccagagacg acttgttggc ttcatccctt acagaaaaaa
      901 agacggtgac gtgcccatcc ttctgcacga gcgcggtggg atagctggca tgacttagcc
      961 accacccacc ggcaggctga ggctcaatca ctcgaccaag acgacgccaa acctgtttcg
     1021 cccgaaccac cattgtcaaa tattacgcaa acgcgtggac ggaaccttac gtttttcgat
     1081 tgttgacgtt ggagcataca ccccttcccg ttcggtgctt tgctgcacga ggcatcctgc
     1141 accaagcaag gtgaatgggg cgatttctat gtgatcgacg atagtcgagt tcacaccgat
     1201 gaagctgttg tggccaaccg tcaccccgcc cgaaatgaca acatgcgacg atataaagac
     1261 gttatcctca ataacagagt ggtgcccgat gtgattacca ctccaaagcg tgacattatt
     1321 gccgatccgg caaaatggct gcagggtatt gtcttcgaag ataagacaat tatcgccgat
     1381 cttatcggtt aagacggttg cgttcgggct gatgtagttg gtcatggtgt agcccaatgc
     1441 gcgcatggca tccaccctct gcgcgcggag cacattcatg cgattatagg acaaggccac
     1501 gaaggcatca tactcggccg gcgggaattg g
//
LOCUS       DATMTI010000446         1817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_568745, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000446 DATMTI010000000
VERSION     DATMTI010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568745"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..526)
                     /locus_tag="VN039_07230"
     CDS             complement(359..526)
                     /locus_tag="VN039_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HWV45796.1"
                     /translation="MLSWAITFLIVAIIAGVLGLSGIAGTATNIAYILFVIFLIVAVI
                     GLVMGRRPPVT"
     gene            complement(572..1357)
                     /locus_tag="VN039_07235"
     CDS             complement(572..1357)
                     /locus_tag="VN039_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV45797.1"
                     /translation="MKLVLVFAPILITLLVLFDRMPSSAQITLWETYQSAGHQAIEDR
                     RVADAERLLLAAAKYIETVSPDDPRLANTLNDLGVLYGMQNRDIEAEPLFEKALAINE
                     KAFGRQHPSVVLALQNLSVIYASRNKFSEAHRAARESLEISLQLFGMYHPRIGSTCRT
                     LATVYALEGKYEEAERFAEKSITVFENTLGERHPETAQSLEMMVRLMWTTHREREAQR
                     LEARLNTIRQSSGSESESAEDTDRQGIKNPNTLREDSRAARRE"
     gene            complement(1421..>1817)
                     /locus_tag="VN039_07240"
     CDS             complement(1421..>1817)
                     /locus_tag="VN039_07240"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HWV45798.1"
                     /translation="RRINLSYEEVDIKLSYELSNWGRLYGGGGYLVHRDPVDVKPVTT
                     QWGLELASPISFFDGSIRPICYADFQVNERANWSVAQSVMAGLRFENARIGNRQLQLL
                     AEYFAGPSPNGQFYVRHEHWFGIGLHFYF"
BASE COUNT          439 a          478 c          474 g          426 t
ORIGIN      
        1 ggttgctcaa aaccgacctt ggtaaatctg tattctttgc gatggtcagg ccggagttat
       61 tcagacggtt gtcactgggg caacgtgtga caatcggaat gaatgaacaa ggccaggccg
      121 tgaaggtcat ggaaattcca ccaagcgaac ttccgattcc gccatcagct gcgcagtagc
      181 tattctcaat ctcacaagga agactggatt cacgattcac acgtgtaaga tttgcgatga
      241 caaacgtcgc aaaggaatgt gatcgggcga aagagccgga cggcttggat catctcctca
      301 cgcgtttacc cgatgtgttc aattaccttc gggtttcggg taaacgcgcc ggaaatcttt
      361 acgtaaccgg cggtcgtcgg cccatcacca ggcctatgac agcaacgatt agaaagatga
      421 caaaaagaat ataggcgatg ttagtggccg tacccgcaat tcccgagagg cctaaaacac
      481 cggcaatgat cgcgacgatc aaaaacgtta tagcccagct cagcatggta cacctcctca
      541 aaagtaagaa ccggtcaaac gacggtatca ctcactctct acgggcggca cgtgagtcct
      601 cgcgcaaggt gtttggattc ttgatgcctt ggcgatcggt atcctcagcc gattcagatt
      661 cggagcccga gctctggcgg atggtattga ggcgggcctc gagtctctga gcctcgcgtt
      721 ctcgatgggt cgtccacatg agccggacca tcatttccaa gctctgagca gtttccgggt
      781 ggcgttcgcc caacgtgttt tcaaagacag tgatcgattt ttctgcaaag cgttcagcct
      841 cttcatactt gccttccagt gcatagaccg tggccagtgt tcggcaggtc gatccaatac
      901 ggggatggta cattccaaat agttgaagac ttatttctaa actttcgcgc gccgccctgt
      961 gtgcctcgga gaacttattc cgcgaggcgt aaatcacact gagattttgc aacgccaaaa
     1021 cgacggaagg atgttgccgt ccgaatgcct tttcattgat ggccaatgcc ttctcaaaga
     1081 gcggctcggc ttcaatgtct cgattttgca tgccatacaa tacgcccagg tcgttgagcg
     1141 tattggccag ccggggatcg tcaggactga cggtctctat gtatttggcc gcggcgagca
     1201 gtaagcgttc cgcatccgcg acacgtcgat cttctatggc ttggtgccct gccgactgat
     1261 acgtctccca caatgttatc tgtgcactgc tcggcatgcg gtcgaacagc acgagcagtg
     1321 tgatcaatat cggtgcaaat acgagtacga gcttcatact gataccctat cgttccaggc
     1381 ccacgcacga tactcggaag ttcccttgat aaagacgcgg tcagaaatag aagtgaagac
     1441 cgatgccgaa ccagtgctca tgtcggacgt agaactggcc gttgggcgag ggacctgcga
     1501 aatattcagc caagagttgt aattgacggt ttccgattcg tgcgttttca aacctcaggc
     1561 ccgccatgac ggattgagcg accgaccaat tcgcacgctc gttgacctgg aagtccgcat
     1621 agcagatcgg cctaatcgac ccgtcaaaga aggagatggg actggccaac tcaagacccc
     1681 attgtgtcgt aacaggtttt acgtcgaccg gatcgcgatg aactaaatat ccgccgcctc
     1741 catacaggcg gccccagttg gacaattcat aggagagttt tatgtcgact tcttcataac
     1801 tcagattgat ccgtcgg
//
LOCUS       DATMTI010000447         1602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_230674, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000447 DATMTI010000000
VERSION     DATMTI010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230674"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VN039_07245"
     CDS             <1..597
                     /locus_tag="VN039_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45799.1"
                     /translation="LHLLPGNVCRADAVPTGGRVLFCQDLQSAPGALIRAGLRYALPA
                     DRAVRPMSWRLRADITPIALQMNEGLVIQPLAFLADDEIVAAACLRKVVGGALFAGVM
                     IRNKDGLFRERIKGGEKGKIAIETAARWEVELLRIGTRQTTVVLRLNDEIVVRINGDT
                     TSVEPDAVGVGVVHKHSGLQITLHVDQLRLTEASRKSG"
     gene            complement(608..>1602)
                     /locus_tag="VN039_07250"
     CDS             complement(608..>1602)
                     /locus_tag="VN039_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV45800.1"
                     /translation="SLFSFHGLSWQDRWKLFSHVEQIWEQAQTLPADLENRTADEWLT
                     TAGQSPEARERIWDPLVQWLAGNALARLSAATFVHLLSTVFLRDASDARLTLLAGSID
                     HRFIIPMKQTLHRAHVRIVSLTHRPTLRFGQTGVSEVRLHDDTRLRADRYVIALSYHH
                     LLQLLPERLLTRYAYFAQITELKSLSEASIQLTCPIPKERPRLLLLSGRPFHQLISTS
                     LDSGEIEYRLSAVGNALAELNNDQLVDAAQTEICELFSGTARADVIVRAVFRERHAAL
                     LLTPGVSRLRPLQQTPIQNLFVTGAWTDTGWPANLESTLASARRCADSVAGRPA"
BASE COUNT          345 a          426 c          511 g          320 t
ORIGIN      
        1 ttgcatctcc tacccggcaa cgtctgccga gcggacgcag ttcccacagg cggccgcgtt
       61 ttgttctgtc aggaccttca aagtgcgcca ggcgcattga ttcgcgcagg gctgcgctac
      121 gccttgcctg ccgaccgtgc cgtccgccct atgtcttggc ggctgagggc cgatatcacg
      181 ccgatagcat tgcaaatgaa cgagggactg gtgatccagc cattggcctt cttggcggac
      241 gacgagatag tcgccgcagc ctgcctgcga aaagtcgtcg gtggagcact cttcgcgggc
      301 gtcatgatcc gcaacaagga tggactattc agagaacgaa tcaagggggg cgagaaaggg
      361 aaaattgcca tagaaacagc ggccaggtgg gaagttgagc ttttgagaat aggaaccaga
      421 caaacgaccg tggtcttgcg attgaacgac gagatcgtgg tccgcatcaa cggtgacacc
      481 acgagcgtcg agcctgatgc cgtcggcgta ggggttgttc ataagcacag cggactgcag
      541 atcacattgc atgtcgatca attgcgcctg acggaggcgt cgcgaaagtc cgggtaagtg
      601 aaccgtttca agcaggacgg ccggcgacgc tgtcggcaca acgacgggcg ctggcgagtg
      661 tgctttccag attggccggc catccggtgt cggtccatgc tccggtgacg aacaaattct
      721 ggatgggagt ctgttgaaga ggccgcagcc gcgagacacc tggtgtaagc aacagggcgg
      781 cgtggcgctc gcgaaagacc gctcggacga tcacatccgc cctggccgtt cccgagaaca
      841 actcgcatat ctcggtctgc gctgcatcga ccaattgatc attgttcaac tcggcgagcg
      901 cgttgccgac ggcagacagc cgatattcga tctcgccgga gtcgagcgat gtgctgatga
      961 gctggtggaa tggtcgacct gagaggagga gcaggcgagg cctttccttt ggaatcgggc
     1021 aggtcaattg aatgctcgcc tcgctcaagc ttttgagttc ggttatttgt gcgaagtagg
     1081 catagcgagt cagcagccgt tccggcagta gctgcaatag gtggtgatag gacagcgcaa
     1141 tgacgtaccg gtcggcccgt aacctggtgt cgtcgtgaag ccgaacttcg ctcacgccgg
     1201 tttgtccaaa ccgaagggta ggccgatggg tcagggagac gatccgcaca tgagcccggt
     1261 ggagtgtctg tttcatggga atgatgaatc gatggtcgat ggaaccggcc agaagtgtga
     1321 gcctcgcgtc cgatgcatct cgaagaaaca cggttgaaag cagatggacg aaggttgcgg
     1381 cggaaagccg tgccagtgcg ttgcctgcca accattgaac gagcggatcc caaatccgtt
     1441 ctctggcttc gggactttgc ccggcagtcg tcagccattc atccgcagtc cggttctcca
     1501 ggtccgccgg gagggtttga gcttgctccc aaatttgctc gacatgggag aagagtttcc
     1561 accgatcctg ccaggaaagt ccgtgaaaac tgaagaggct ca
//
LOCUS       DATMTI010000448         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_653795, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000448 DATMTI010000000
VERSION     DATMTI010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653795"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..841)
                     /locus_tag="VN039_07255"
     CDS             complement(134..841)
                     /locus_tag="VN039_07255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWV45801.1"
                     /translation="MAESIRETTVQYPSGKVVTMRAFVAAPHTKDKRPAVIIVQEWWG
                     LTDHIKDIARRYSGEGYVAIAPDLYSRLGHPLTMDAGEAGKLMNTLKQEDGLNDLNET
                     VHYLKSVPEVDATKIGVTGFCMGGSYALMLPCVNPEIKVAVPFYGQVPNPDTPIQKLA
                     CPVLYLYGEDDGWITKADVQRLATALKKYGKAGEIKTYPGAPHAFFRDTDPSVYRPDA
                     AQDAWARAKAFFKLHLG"
     gene            complement(950..2326)
                     /locus_tag="VN039_07260"
     CDS             complement(950..2326)
                     /locus_tag="VN039_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWV45802.1"
                     /translation="MLQKYTTPDAANGYAELAADVLAHARACGATEADIVVADGETLS
                     VQVRVGTVDRLTKAREKRLGLRVFIGKRSATTSTSDFSQESLERLVGDTCTLARAVVE
                     DDVSGLPDAAQMAKEQPNLDLYDETVLDTETQIDWAKRGEAAAFAMDPRITNSEGAEF
                     DSSSGRVVLANSHGFVGSYKSSNFSLSVSPIATESGTGAMQRDAWYDVQRKFARLASA
                     ESIGQEAARRAVRRLGARKVSTKRVPVVFDQETAGSLLANLCSAVSGYGLYKRASFLL
                     DKLGQVVASDLMTLYDDGRMVGGLGSRPFDGEGLATRKNTIVERGVLKSYLLDTYSGM
                     KLGLPSTGNASRSVGESPSVGPTNFYLVPGLKSPEEIISSVKEGLYVTELIGFGINMV
                     TGDYSRGASGFWIENGELAYPVEEITIAGNLKHMFKDIEIIGSDLVFRGRIASPTLKI
                     SEMMVAGN"
     gene            complement(2421..>3084)
                     /locus_tag="VN039_07265"
     CDS             complement(2421..>3084)
                     /locus_tag="VN039_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWV45803.1"
                     /translation="EAIGHGLEADFNRKKTSAFSNLIGKRVASDVCTIVDDGTLPFRR
                     GSLNIDDEGTPTSCTMLIERGILRRYITDKLNARLMGISLTGNGRRENYQSVVLPRMT
                     NTFMLAGESDPQDIIRSVKKGLYAVSFGGGQVDITNGKFVFSASEAYLIEDGRITTPV
                     KGATLIGNGPEILTKVSMVGHDLKLDNGIGTCGKDGQSVPVGVGLPTIKIDEITVGGT
                     QR"
BASE COUNT          639 a          930 c          790 g          725 t
ORIGIN      
        1 ccgggagtga gcgcaatcgc caaaagaatg ctgctccatg tcagcctcat acctcgtcct
       61 cactctccca ggatattgaa aacggttcgc cggctgtatt ctctgtcgcg ccctgcattc
      121 tctccatcac aagctagccg agatgcagct tgaagaaggc cttggctctg gcccaggcgt
      181 cctgagctgc gtcgggccga tacacggatg gatcggtgtc tcggaaaaat gcatgaggag
      241 caccagggta agttttaatt tcaccggctt tcccatactt cttcaatgcc gtggccagtc
      301 gttggacatc cgccttggta atccagccat cgtcctcgcc gtaaagatac aacaccgggc
      361 aggccagctt ctgaatcggt gtatcgggat tgggcacttg gccgtagaac ggcactgcga
      421 ctttgatttc tggattgacg catggcagca tcagcgcata ggagcccccc atgcagaatc
      481 ctgtgacacc gatctttgtc gcgtcgactt ccgggactga tttcagataa tgaacggtct
      541 cattcagatc gttgagtccg tcctcctgtt tcagggtatt catgagcttg ccggcttcgc
      601 ccgcatccat agtaagcggg tgtcccaggc gcgaatagag gtccggagca atcgcaacgt
      661 agccctctcc ggaatatcgt ctcgcgatat ctttgatgtg atccgtaagg ccccaccatt
      721 cctggacgat gatgacagcg ggtcgtttat ccttggtgtg gggggcggcc acgaatgccc
      781 gcattgtcac cacttttcca ctcgggtact gaacggtcgt ctctcggatg gattcagcca
      841 tgagtcacct cggataagag cgtatggctg atagcttatg gcgaatagca aaacctcttt
      901 ccggtctcat ggttcttgcc atcagccatc ggctatacgc catatgctcc taatttcctg
      961 ccaccatcat ctcggagatt ttcaatgtgg ggctcgcaat acgtcctcga aatacaagat
     1021 cgcttccgat tatttcgata tccttgaaca tgtgtttcag attgccggcg atggtgattt
     1081 cttcgacggg gtaggctaac tctccgtttt cgatccagaa cccgctggcg ccgcgcgaat
     1141 aatcgccggt gaccatatta atcccaaacc cgatcagctc ggtgacatag agcccttcct
     1201 tgactgaact aatgatctcc tcagggctct tcaacccggg gacgagataa aaattcgtcg
     1261 ggccgacgga aggactctcg cccacgctcc gtgacgcgtt gccggtcgac ggcaaaccca
     1321 gtttcattcc ggaataggta tcgagcaggt aactcttcag tacgccgcgt tcgacgatcg
     1381 tattcttccg tgtggccaac ccttcgccgt caaacggacg agatccaagg ccgccgacca
     1441 tccggccgtc gtcgtacagg gtcatcaagt cggatgcaac cacctggccc agtttgtcga
     1501 gaagaaacga tgcccgcttg tagagcccat agcccgacac ggcactgcat aagtttgcca
     1561 gcaggcttcc ggccgtttct tgatcgaaca caaccggcac tcgtttcgtc gacaccttgc
     1621 gggcgcctag gcggcgaacg gctcgcctgg ccgcctcttg cccgatcgat tctgcggatg
     1681 ccaaccgtgc aaacttgcgt tggacatcgt accacgcatc tcgctgcatg gccccggtgc
     1741 cggattcggt cgcaatcggg gagacggaca gtgaaaaatt tgaactcttg taggacccga
     1801 cgaatccgtg gctgttcgcc agcaccacac gccctgacga cgaatcgaac tccgcacctt
     1861 ccgagttcgt gatgcgcggg tccatggcaa aggccgccgc ttctccgcgc ttggcccaat
     1921 cgatttgcgt ttccgtatca agcaccgtct catcatagag atcgagattc ggctgttctt
     1981 tggccatctg agccgcatcc ggtaaccctg atacgtcatc ctcaaccacc gccctggcca
     2041 aggtacaggt gtctccaaca agccgttcga gggattcttg cgagaagtcg gatgtggaag
     2101 tcgttgccga tcgctttcca atgaagacac gcagccctaa ccgcttctct ctcgccttcg
     2161 tcaatcgatc gaccgtgccg acccgcactt ggaccgaaag ggtctcacca tcggctacca
     2221 cgatatcggc ctccgtcgcg ccgcaagctc tcgcatgagc caggacgtcg gcagccagct
     2281 cggcataccc attggcggca tctggagtcg tatatttctg gagcatcgct gggcttcgtc
     2341 tatttcttca tcagtcaatt catcagtcaa ccatcttgct tgcgatcgat tgacgaacaa
     2401 acattgatga tgaacgcctt ctatctctgt gtcccgccga ccgtaatttc atcgattttg
     2461 atggtcggca agccgacccc aaccggcacc gattgaccgt ccttgccgca cgtgccgatg
     2521 ccattgtcga gtttcagatc gtggcccacc atcgatacct tggtgaggat ctccgggcca
     2581 ttcccgatca atgtggctcc cttcaccggc gtcgtgatcc gcccgtcctc gatgaggtag
     2641 gcctcgctgg cagaaaacac gaacttcccg ttggtgatat caacctgccc accgccgaac
     2701 gagacggcat acagcccttt tttcaccgat cggatgatgt cttgaggatc cgactcaccg
     2761 gccaacatga aggtattcgt cattcgagga agcactacgc tttgataatt ctcccgccga
     2821 ccgttaccgg tcagggaaat ccccatgagg cgggcgttga gcttatcggt gatatagcgt
     2881 cgaaggatgc ccctctcgat aagcatggtg cagctggtcg gcgttccctc atcatcgata
     2941 ttcaacgagc cgcggcgaaa tggaagagtt ccatcatcga cgatcgtaca gacgtccgac
     3001 gccacgcgtt tcccgatcag attcgagaag gccgatgttt ttttcctgtt gaaatcggct
     3061 tccaagccat gtccgatcgc ttcg
//
LOCUS       DATMTI010000449         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_230747, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000449 DATMTI010000000
VERSION     DATMTI010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230747"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..984)
                     /gene="pstA"
                     /locus_tag="VN039_07270"
     CDS             complement(94..984)
                     /gene="pstA"
                     /locus_tag="VN039_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWV45804.1"
                     /translation="MLRRRKLAEALFKTVGICSLALGIGTLVLLFGALIVQGAGRLDW
                     QFLTSFPSRRATQAGILPALVGSSLIMLVTAVVGIPLGVAAAVYLEEYARRTWLTELI
                     EVTVTNLAGVPSIIFGLLALGFFVYILGLGASILTAGLTLALLILPVVIVTTREAIRA
                     IPVQIREAAFALGATKWQTVSDHVLPYSSAGILTGIILALSRAIGETAPIVTIGALTF
                     IAFLPPAPFATEPPFISFEWLMSPFTVMPIQMFAWVSRPGEDFASNAAAAGLLLVCMT
                     LGMNGLAIYVRYRMRKRIAW"
     gene            complement(984..1940)
                     /gene="pstC"
                     /locus_tag="VN039_07275"
     CDS             complement(984..1940)
                     /gene="pstC"
                     /locus_tag="VN039_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWV45805.1"
                     /translation="MNKSLAAVREVIRISEPVIVEATARRRTQHRREQVIESGLFLAA
                     MMSVAATVAILTVLLWESLGFFQAVSLTDFLTDTLWTPLFDDAHYGILPLLAGTVVTS
                     LVGLLVAIPVGTVSAIYLSEFASSRLRELIKPVLELLGAVPTVVYGYFALLVVTPALQ
                     KLWPDLPGFNMLGPGIVIGIMILPLVISLSEDAMRAVPMVLREGSYATGATRLQTAWR
                     VVVPAATSGLVAAYVLGVSRAVGETMVVAIAAGQNPNLTWNPMEPAATITAYIVQVAL
                     GDLPHGSIGYQSIFAAGLILAAMTFTFNMLGHVMRKRFREVY"
     gene            complement(2004..>2989)
                     /locus_tag="VN039_07280"
     CDS             complement(2004..>2989)
                     /locus_tag="VN039_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWV45806.1"
                     /translation="MIAAGVWATFVLASHPAQADQSAMIKVDGSSTVYPLTEAVAEEF
                     QNANRGKIRVTVGIAGTGGGFKKFCRGEIDIADASRPILKEEMALCQKNGITYYELPI
                     AFDALTVAVSPLNTWVTSMTIEELKRIWEPAAQGKITKWNQVRSDWPDVQLVLFGAGS
                     DSGTFDYFTDAVVGKAKSSRGDYTASEDDNVLVQGIERNKNALGYIPFAYYAAQMKKL
                     KAVAVTGKNGAILPSAESVVNGSYQPLSRPLFIYVSESSAKRPEVKQFIEYYLAEGPK
                     LIAEVRYIPLADQAYGMARERFQKSIVGTGFGGEPEVGLPVEEIMKRSPKQ"
BASE COUNT          738 a          886 c          777 g          588 t
ORIGIN      
        1 gaaaaactca agctgcgggc ctcagccttg agaggcttag gcaaaacccg atcctcgggt
       61 aattcgatac gtggtaactc catgaggtcc atattaccaa gcgatacgct tgcgcatgcg
      121 ataacgtacg taaatggcca ggccgttcat tccgagagtc atgcagacca gcaacaatcc
      181 tgcagccgcc gcattgctcg cgaagtcttc gcccggcctt gaaacccatg cgaacatctg
      241 aatgggcatg accgtgaagg gagacatcaa ccactcgaac gaaataaacg gaggttccgt
      301 cgcgaatggg gccgggggta gaaaggcgat aaaggtcagg gcgccgatcg tcacgatggg
      361 tgccgtctct ccgatcgccc ggctcaaggc caagatgatc ccggtcagga tcccagccga
      421 ggaatagggc aacacgtgat cggagaccgt ctgccatttg gtcgcaccca aagcaaaggc
      481 cgcttcgcgg atctgcaccg gaatcgcgcg aatagcctct cgtgtcgtca cgatcacgac
      541 gggaagaatg agcagggcca aggtgagacc agccgtcaga atgctcgccc caagacctag
      601 aatatagacg aaaaacccta gcgccagtag gccaaatata atcgatggca cgccggccag
      661 attcgtaacc gtcacctcga tcaactccgt cagccacgtt cggcgggcat attcctccaa
      721 gtaaaccgcc gcagcgaccc ccaaggggat gcccaccacg gccgtgacca gcatgatcaa
      781 actcgagccg accaacgcag gaagaatacc cgcctgtgtg gcgcgacgag acggaaagga
      841 tgtgagaaat tgccagtcga gtcggccggc cccttgaacg atgagcgcgc cgaacaacaa
      901 caccagcgtt ccgatcccga gggcgaggga acagatcccc acggtcttga acaacgcttc
      961 cgcaagctta cggcgtctca acatcaatag acctcacgga accgtttccg catgacatgg
     1021 cccagcatgt tgaaggtaaa cgtcatcgcc gcaaggatga gcccggcagc gaaaatgctc
     1081 tggtagccga tactgccatg agggagatca ccaagggcca cttgcacgat ataagcagta
     1141 atggtggctg caggctccat gggattccac gtgagatttg gattttgccc ggctgcgatc
     1201 gctaccacca tcgtttcacc gacggcacgc gagactccga gcacataggc cgccacaagg
     1261 ccggaagtgg ctgccgggac taccacacgc caagctgtct gaagacgtgt cgctcccgtc
     1321 gcataggatc cttcccgcaa caccatgggc acagcccgca tcgcatcctc actcagagaa
     1381 ataacgagag gcaatatcat gatgccgatg acgattccag ggcccagcat attgaagcca
     1441 gggaggtccg gccaaagctt ctgcagagcg ggcgtgacca caagaagcgc aaaataccca
     1501 tacacgactg tcggtaccgc tccgagcagt tccaacaccg gcttgatgag ttctcgcaag
     1561 cgagacgagg caaattccga gagatagatc gccgacaccg tccccacggg aatcgcaacg
     1621 agcagaccga ccaggctggt gactaccgtc cctgcgagca ggggcaggat tccatagtgg
     1681 gcatcatcaa agagcggtgt ccacaatgta tcggtcaaaa agtccgtgag cgacacggcc
     1741 tggaagaacc cgagcgattc ccacaagagc acagtgagaa tcgcaaccgt ggcggctacc
     1801 gacatcatcg cggcaaggaa aagcccgctt tcgatcactt gttctcgccg gtgttgagtc
     1861 cgacgccgag cggttgcttc tactatgacc ggttcgctga tacgaatcac ttcccgcaca
     1921 gccgctagag acttattcat gaacagccaa cctttttctg tatcctcctg tatccctcga
     1981 tgcaagtcaa acgtgggcgc tacctattgc tttggggagc gcttcataat ctcttcaacc
     2041 ggcagtccga cttcgggttc accgccaaag ccggttccta caatactttt ttgaaatcgc
     2101 tcacgggcca tcccatacgc ctggtcagcc aatggaatat atcggacttc tgcgatcagc
     2161 ttgggacctt cggcgagata gtactcgatg aattgtttca cttccggacg tttagccgaa
     2221 ctctcactga catagatgaa gagaggacgt gaaagcggct gataactgcc gttcaccaca
     2281 ctttccgcac taggcaatat cgctccattc ttgcctgtca cggcgactgc cttgagcttt
     2341 ttcatctgcg cggcatagta ggcaaacggg atatatccta atgcattctt gttcctttca
     2401 atcccttgga ccaacacgtt gtcgtcctcg ctggcggtgt aatcaccgcg gctggacttg
     2461 gctttcccca caaccgcatc ggtaaagtag tcaaaggttc cggagtccga accggcccca
     2521 aaaagaacga gctggacgtc gggccaatca gaacgaactt ggttccattt ggtgattttc
     2581 ccttgtgccg ccggttccca gatccttttc aactcttcga tcgtcatcga agtcacccag
     2641 gtattcaggg gactgactgc gaccgttaac gcatcaaatg caatgggcaa ctcatagtag
     2701 gtgataccgt tcttctggca gagagccatt tcttccttca aaatggggcg ggatgcatcg
     2761 gcgatatcga tctcgcctcg acaaaacttc ttgaacccgc cgcctgtccc cgcaataccg
     2821 acggtcactc gaatcttccc gcgattggca ttctgaaatt cttccgccac cgcttcggtt
     2881 aagggataca cggtactgga gccatcaacc ttgatcatgg ccgattgatc ggcctgagct
     2941 ggatgacttg ctaaaacaaa cgtcgcccat acgccggccg cgatcatac
//
LOCUS       DATMTI010000450         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_230749, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000450 DATMTI010000000
VERSION     DATMTI010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230749"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VN039_07285"
     CDS             complement(<1..186)
                     /locus_tag="VN039_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWV45807.1"
                     /translation="MLSDTERRELEEALKHYPDKRSGTIDALLIVQRRRGWISDDSLL
                     DIAQFLEMTSEDVDSVAT"
     gene            complement(262..2058)
                     /gene="nuoC"
                     /locus_tag="VN039_07290"
     CDS             complement(262..2058)
                     /gene="nuoC"
                     /locus_tag="VN039_07290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF008728.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HWV45808.1"
                     /translation="MTPRSSTIREASTGERTGVVAELESRFRSEGWLRAVQHTKDEIP
                     TIWVEKHRLRETLRYVKSDIPRPFPMLFDLSATDERLRRHHDGLPIGAFTVFYHLFSF
                     DRNQSLRIKVPLGGEDLSLPSIVDLWPNADWYEREIYDMFGIRFEGHPFLRRLLMPSW
                     WNGHPLRKDHPSRATEMGQFQLPPEKLVMTQEALQFKPEDWGLSRTHQDPDFDYMFIN
                     LGPAHTGTHGVLRLVAQLAGEEIVNIVPDIGFHHRSQEKLAERQTWHTFIPYTDRVDY
                     LQGVLNEMPYCLAVERLADIEVPQRAQVIRVMMSEFYRLASHLVWYGTFAQDIGALSP
                     VFYMFNDRERILAISEAICGGRMHPNWLRIGGVARDLPIGWDQLVRQFIDWFPSRLDE
                     YERLVMDNPIFKARTVGIGRLSLEDAIAWGATGPMLRACGLPWDLRKTRPYSGYDQFD
                     FDVPVATNGDCYDRGVVHVLEMRQSLRIIRQCLDSMPSGPYKSLDPLATPPMKEHTMH
                     DIETLIHHFVGVSWGPVLPPGEAEVPTESSKGQTSYYVTSDGSQVSYRTRIRTPSFAH
                     IQMVPFIARGELLSDLLAVLGSVDYVLSDVDR"
     gene            complement(2055..>2430)
                     /locus_tag="VN039_07295"
     CDS             complement(2055..>2430)
                     /locus_tag="VN039_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45809.1"
                     /translation="EPRWVISMGSCSNSGGMFDAYSVVQGVDKFLPVDVYMPGCPPPP
                     HAFMECLLLLQNMVGSERRPLSWWFGPQEVQKPIMPSMRDAKRAERMSQREYPGIDVL
                     PRTDLLSPHFAGQPNGLSKAKL"
BASE COUNT          566 a          673 c          674 g          517 t
ORIGIN      
        1 ggttgcgaca ctatctacat cctcgctcgt catctccaga aattgcgcaa tatcaagaag
       61 cgagtcatct gaaatccacc cgcgtcggcg ctgtacgatc aacagcgcat cgatggtccc
      121 cgatcgctta tcagggtagt gtttcagtgc ctcttcaagc tcacggcgtt cagtgtctga
      181 caacataagt gctgagttct gagtttcgga acccattgac ttcgtgctca gcattcagca
      241 ctgagcactc tgcattgttt ttcaccggtc tacatccgac aacacgtagt ccacacttcc
      301 aagcacggct aaaaggtccg acaacagttc gccgcgtgcg atgaacggga ccatctgaat
      361 atgggcaaat gagggggtgc ggattcgcgt tcggtaggag acctgcgatc catcactcgt
      421 cacgtaataa ctcgtctggc cttttgacga ttccgtagga acctccgcct caccgggtgg
      481 aagcaccggt ccccaactca ccccgacaaa atgatggatg agtgtttcga tgtcgtgcat
      541 cgtgtgttcc ttcattggtg gagttgcgag cggatcgagc gacttatagg ggccggaggg
      601 catcgagtcg agacactggc ggataatccg cagcgactgg cgcatttcca gcacatgcac
      661 cactcctcga tcatagcaat cgccgtttgt cgcgaccggg acatcaaagt caaactgatc
      721 atacccggag taaggacgag tcttgcgcaa atcccagggt aagccgcagg cacgcaacat
      781 tggaccggtc gcaccccagg cgatggcgtc ttcgagtgat aatcgaccga tcccgacagt
      841 gcgcgctttg aaaattgggt tgtccatcac gaggcgttca tactcatcga gtcggcttgg
      901 aaaccaatcg atgaactgac gaaccagttg atcccatcca atcggaaggt cccgtgcgac
      961 tccaccgatg cggagccaat tcgggtgcat acggccgcca caaatggctt ccgagatcgc
     1021 aagaattcgc tcgcgatcat tgaacatata aaagaccggc gacaaggcgc cgatgtcctg
     1081 tgcaaaggtt ccgtaccaga cgagatggct ggcgagccga tagaattcgg acatcatgac
     1141 gcggatgact tgtgctcgtt ggggtacctc aatgtctgcg agtcgttcga ctgcgaggca
     1201 gtacggcatt tcattcaaca caccctggag atagtccact cgatcggtgt agggaatgaa
     1261 ggtatgccag gtctgccgtt cggcgagttt ttcctgggac cgatggtgaa atccgatatc
     1321 aggcacgatg ttgacaattt cctcacccgc gagttgcgcc acgagccgca gcacgccatg
     1381 cgtaccggta tgggcagggc cgagattgat gaacatatag tcgaaatccg ggtcctgatg
     1441 ggtgcgggat aagccccaat cctccggctt gaattgcaag gcctcttggg tcatcacgag
     1501 tttctcaggc ggtaattgaa actgccccat ctctgttgca cgcgagggat gatccttgcg
     1561 aagcggatga ccgttccacc acgaaggcat gagaagacgc cggagaaatg ggtgcccctc
     1621 gaatcgaata ccgaacatgt cgtagatttc tctttcgtac caatcggcgt tcggccacag
     1681 atcgacgatc gacggcagtg agagatcttc accccccagc ggaaccttga tacgaagcga
     1741 ttggttacga tcaaaagaaa agaggtgata gaagacggta aaggcaccga tcggcaatcc
     1801 atcgtggtgt cggcgtaatc gttcatccgt cgcactcaag tcgaacagca tgggaaacgg
     1861 gcgaggaatg tccgacttga catagcggag cgtctcacga agccggtgct tctcgaccca
     1921 aatcgtcggg atctcgtcct tagtgtgttg tacggccctc agccagcctt ctgaccggaa
     1981 gcgactctcg agctctgcca ccactcccgt ccgttcacca gtcgacgcct cccgaatggt
     2041 cgatgatcgt ggagtcatag cttcgctttt gagaggccgt ttggttggcc tgcaaagtgg
     2101 ggggagagca gatctgtccg aggaagcaca tcgatgccgg gatactcgcg ttgactcatc
     2161 cgctctgctc gtttcgcatc ccgcatcgaa ggcatgatgg gtttctgaac ttcttgcggg
     2221 ccgaaccacc agctcaaggg ccgacgctcg gatccgacca tgttctggag gagcagcagg
     2281 cattccataa aggcgtgcgg aggcggggga catcccggca tatagacgtc aaccggcagg
     2341 aatttatcga caccttgtac gacagaatag gcgtcgaaca tccctccgga attcgagcag
     2401 gagcccatcg agatcaccca acgaggctcc
//
LOCUS       DATMTI010000451         5006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_271434, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000451 DATMTI010000000
VERSION     DATMTI010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271434"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /gene="metG"
                     /locus_tag="VN039_07300"
     CDS             <1..795
                     /gene="metG"
                     /locus_tag="VN039_07300"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HWV45810.1"
                     /translation="TLSLLNEVIPRHFDQLEFNRVLEAIWHVVQLANQYVDKTAPWNL
                     AKNQSDAEQLRTVLYTMAETLRCISLAAYPVIPKSAQSICSQLGISAEFTSPLLRSKI
                     EWGGLKPGTLIHKGPSLFPRIELKPGARPVTESSVPSQPTPTAPTATAVPAPPAPPQI
                     TIDEFMKIQLKTAKVLSAERVPKSEKLIKLQVSLGTEQRQIVAGIGKKYEPDALIGKT
                     IVIVANLKPAKLMGIESQGMVLAAGDTDVQGLLTIQEEVDSGTKVK"
     gene            792..1646
                     /locus_tag="VN039_07305"
     CDS             792..1646
                     /locus_tag="VN039_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45811.1"
                     /translation="MTRPSFAPLGLIFSLTLLLCFPTPATSQALADSSLNEPQPTTVL
                     VRVVAHGAMVLGREVGGARVTITDVASGHILATGLQQGESGDQNQIMRTPHLMEEPIY
                     SARPSAAFTTTLELQKPTVVEISAEGPLAYPAALQRASKTLLLVPGQDLTNDGIVLHL
                     YGYLVQIERPKPREALIAKDDVKLRASVRTLSGSLVRPHGDWDSRKIRIYGEVLIGDR
                     IIERLQLFYDEGSRAFEAPFFVPPSKEVPDGIKLRVIATDLATGNSGIDQANYPVLSE
                     RLPPKSVR"
     gene            1721..2656
                     /locus_tag="VN039_07310"
     CDS             1721..2656
                     /locus_tag="VN039_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWV45812.1"
                     /translation="MTAVASYAHLRSRLQLALAINAVIIAAEFVGGWLLNSIGLMSDA
                     GHNLVDQGSLFLALYAHLLTARPASESRTFGYHRAGIIAAFLNSFILLLTALGITLVG
                     VKRLLQPVPIDGEWVMAVAAVSFVANLGIALLLQHGAEDDLNIRSAFWHMVGDAWVSL
                     GVIVSGAAIFLTGWTVLDPLVSLLVVGAIVRGAWPIFKESMEVLLESTPPGISASHVA
                     ATMEAIPGVRNVHDLHIWAVEPRLVMLTSHVMIEHHRTPLELLQLIQNRITTDFGIKH
                     MTIQLETECCDPDDIHCDLRKLTEHHEADALAHHH"
     gene            2796..3572
                     /locus_tag="VN039_07315"
     CDS             2796..3572
                     /locus_tag="VN039_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV45813.1"
                     /translation="MSGGVEREIVLRAQPMNTPQPLVLIFHGGGGSAQFMARRSSSFA
                     GLLLRQGYAVAFMNGSSRRDGQNLRTWNGGHCCAYAMTTKIDEADYIDQAVTVIAAQA
                     PIDRSRIFLMGHSNGGMIAYRWPTALHTKVRGIVVISSAMFADQPVMPHGTSAFLTHT
                     LDDDNVAFDASFAPKQKRAWNAPPLPFPEAEARMAEMLGCGSPKEQKVTDGVTRRDHA
                     CAKGTELTVIVSTRGGHAWPKSIPGFSLEEAILTFLDRQR"
     gene            3996..4283
                     /locus_tag="VN039_07320"
     CDS             3996..4283
                     /locus_tag="VN039_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45814.1"
                     /translation="MKFITVRDLRGRSGQVWTKLAREREIILTSNGKPIAILSAVSED
                     TLEESLTAVRRARAVAAVEKMQRQSVQFGTDKISPREIAAEIKAVRKAHRR"
     gene            4280..4687
                     /locus_tag="VN039_07325"
     CDS             4280..4687
                     /locus_tag="VN039_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HWV45815.1"
                     /translation="MNVVVDTNVLIAGLLSPFGPPGEIVRMMATGTLRVCYDARILTE
                     YGEVLARPMFRFHPEQIQALLDQIKAEGLSVAGHLLPARLPDHTDEPFLEIALAGKAD
                     CLITGNAKHFPFGKRQGIAVLSPREFLERYRKR"
BASE COUNT         1140 a         1491 c         1314 g         1061 t
ORIGIN      
        1 acgctgtctc tcttgaacga agtgatacca cgccacttcg atcagttgga atttaatcgg
       61 gtgctcgaag ccatatggca cgtcgttcag cttgccaatc agtatgtcga taagaccgcc
      121 ccgtggaatc tggcaaagaa ccaaagtgac gcagaacaac tgaggaccgt cctctatacc
      181 atggctgaga cgctccgctg catcagcttg gccgcgtatc ctgtgatccc gaagagtgcg
      241 cagtcgatct gttctcaact tggcatctca gctgaattca caagcccttt gctgagaagc
      301 aagatcgaat ggggtggcct gaagcccggc accctcatcc ataaaggacc gtcgctgttc
      361 ccccgcatcg aattaaaacc aggagcaaga ccagtgaccg aatcatctgt tccttcacaa
      421 ccaaccccaa cagcacccac cgccacagcc gttccggcac ctccggctcc gccgcaaatc
      481 acgatcgacg aattcatgaa gatacagctc aagacggcga aggtactctc cgcagaacgt
      541 gtgccgaaat cggagaagct gatcaagctt caagtatccc tcggcaccga acaacgtcaa
      601 atcgtggccg gcatcggcaa gaagtacgag ccggacgcgc tgatcggcaa aaccatcgtg
      661 atcgtggcca acttgaagcc ggccaagctc atgggcattg aatcgcaggg tatggtgctc
      721 gcggcaggtg ataccgatgt acaggggttg ctcaccattc aggaagaagt ggattccggc
      781 accaaggtga aatgacgcga ccttcgtttg ccccgcttgg actgattttc tcgctcacct
      841 tgctcctttg ctttccgact cccgccacaa gccaagccct cgctgattca tcgctcaacg
      901 agcctcaacc gaccacggtg ctcgtgcggg tcgtcgcgca tggagcaatg gtgttgggcc
      961 gtgaagtcgg tggagcgcgc gtgaccatta ctgatgtcgc gagcggccac attctggcaa
     1021 ccggcctgca acagggcgaa tcaggagatc agaatcaaat catgcgtacc ccccatctga
     1081 tggaagagcc gatctacagc gctcgtcctt ctgccgcctt caccaccacg ttggagctgc
     1141 agaaaccgac agtagtagag atctccgcgg aagggccgct cgcctatccg gctgcattac
     1201 agagggccag caaaaccttg ctgttggtcc cgggacagga cctgacgaac gacggcattg
     1261 tcctgcatct ctatggctac ctggttcaga tcgagcggcc caagccacgt gaggcattaa
     1321 tcgcaaagga cgatgtgaaa ctccgagcct cagtccgaac cctgtccggt tcattggtcc
     1381 gcccccatgg agattgggac tcgcggaaga tccgtattta tggagaggta ttgatcggag
     1441 accgaatcat cgagcgacta caactgtttt atgacgaagg gagccgcgcc tttgaagcgc
     1501 cgttctttgt gccgccgtcg aaggaggttc ccgatggcat caagcttcga gtgattgcga
     1561 cggacctggc tacaggcaat tcaggaatag atcaagccaa ctatcctgtg ctaagcgagc
     1621 gtctcccgcc caaatccgtt cggtagccgg ttgcaacttc cgtgatagaa accttcggca
     1681 aggcttgctg cctcgcgttc tccgtgcgaa actatcattc atgacggcag tggcttccta
     1741 tgcacacctc cgctctcggc tccagctggc cttggccatc aacgccgtaa tcatcgcggc
     1801 tgagttcgtg ggcggctggc tcctcaacag catcgggctc atgagcgacg ccggtcacaa
     1861 tctggtcgat caagggtcgc tctttctggc actgtatgcg cacctcctca ccgcgcgccc
     1921 ggcgtcggag agccgaacct tcggctacca tcgtgccggc atcatcgcgg cttttctcaa
     1981 cagttttatt ctactattaa ccgcactcgg catcacgctc gtcggcgtga agcgactcct
     2041 gcaaccggtt cccatcgacg gtgaatgggt gatggctgtc gcggccgtca gtttcgtggc
     2101 caatctcggg attgctctgt tgctccagca cggagcggaa gacgatctga atattcggag
     2161 cgcattctgg catatggtgg gagatgcctg ggtgtcgctc ggcgtcattg tcagcggtgc
     2221 agccattttc ctgacaggct ggacggtcct ggatccgctc gtgagcttgc tggtggtcgg
     2281 ggcaatcgtc cgaggagcct ggccgatctt caaagaatcg atggaggtct tactggaatc
     2341 gacaccgccg ggcatcagtg catcccatgt cgcagccacg atggaagcca tccccggcgt
     2401 gaggaacgtc catgatctgc acatttgggc ggtcgagccc cggctggtca tgctcaccag
     2461 ccacgtcatg atcgaacatc atcgcacgcc gctggagctg ctgcagttga tccagaaccg
     2521 gatcacgacg gatttcggca tcaaacacat gaccattcaa ttggaaaccg agtgctgcga
     2581 ccccgacgac atccactgcg accttcggaa gctgaccgag catcacgaag cagacgcgct
     2641 tgcacaccat cactgatctc aaattcagtt tcgattcgac tgacacaaat cgtgatgaca
     2701 agagttctca tcacaatcgg cctcctcgct cttgccacga gcaccgttgc gcatcggcgg
     2761 cggatcgctt gtcttccgat accgtgcttc cagtcatgtc cggcggcgtt gagcgtgaga
     2821 tcgtcttacg tgcacagccc atgaacacac cgcaaccgct ggttctgatc tttcatggtg
     2881 gtggcggcag cgcccagttc atggcccgac ggtccagttc ctttgctggc ctgctcctac
     2941 gtcagggcta tgccgtcgcg ttcatgaacg gttcctctcg ccgcgatggt caaaacctgc
     3001 gcacctggaa cggtggacat tgctgcgcct atgccatgac caccaaaatc gacgaagccg
     3061 actacatcga ccaagccgtc accgtcatcg ccgcacaagc gcctatagac cgttcacgaa
     3121 tcttcctgat gggccattcc aatggcggta tgatcgccta tcgatggccg accgctctac
     3181 acacgaaggt acgtggtatc gtggtgatct cgtcggccat gttcgccgac caaccggtca
     3241 tgccccacgg cacctcagct tttctgaccc acaccctgga cgacgacaat gtcgccttcg
     3301 atgcctcctt cgcaccaaaa cagaagcgag cctggaatgc gccgcccctt ccgtttcccg
     3361 aggcagaagc tcgtatggct gaaatgttag gctgcggcag cccgaaagag cagaaggtga
     3421 ctgatggtgt gactcggcgg gatcacgctt gcgctaaggg tacagaattg actgtcatcg
     3481 tatcaacgag gggcggtcat gcatggccca agtccatacc gggcttcagc ctggaagagg
     3541 ccatcctgac gtttctcgat cgccaacggt agatgtacga acgttactga atccactttc
     3601 cattcatatc gcgtaacgca gctcaaacac tacccggtag ggcctgcatt agacggtctg
     3661 tgggcgatcg gagcaaagag gagtacgagc agtagaaccg tgagtgcaca ttcacgcgcc
     3721 ggttattttc agaccattcc agcttcgaac catcgcaggt ttttaataag tcactactat
     3781 gccagacttc atttctgata cgaatgctgc gacgcggacg acattcacta cgaccttcgg
     3841 aagctcaccg gacatcacca cgaaacacac accttcgcgc accatcattg acgataagtc
     3901 aactctgacc ctcagcatga tgtgaagggg aagagacgct tgtttgacga tcggctctcg
     3961 atacggtact ctattgtcac tcaaaggcag gagacatgaa attcattacc gtacgagact
     4021 tgcggggtcg ttctggccaa gtatggacca agcttgctcg tgaacgagag atcatcctca
     4081 catcgaacgg caagccgatc gccattctct ctgccgtctc ggaagacact cttgaggagt
     4141 cgctgacagc cgtcagacgt gcgagagccg tcgctgctgt ggagaagatg cagcgacagt
     4201 ccgtccaatt cggcacggac aaaatttctc cgagagaaat cgccgctgag attaaggccg
     4261 ttcggaaggc tcaccgccgg tgaacgtggt ggtcgatacg aatgtcctta ttgcagggct
     4321 tctatcgccg tttggaccac ccggtgaaat tgtcagaatg atggccacgg gtactttacg
     4381 cgtgtgctac gatgccagaa tacttacgga atatggcgag gttcttgcca gaccaatgtt
     4441 cagattccat cctgagcaaa ttcaggcgtt gctcgatcaa attaaagcag agggattgag
     4501 tgtagcagga catctattgc cagccagact tccggatcac accgatgaac cctttctgga
     4561 aatcgctctc gccggcaaag ccgactgcct gattacagga aacgcaaaac attttccatt
     4621 cggcaagcgt caagggatcg ccgtgctttc cccaagagaa tttctcgagc gttaccgtaa
     4681 gcgatagagg aaaggtgaag acctgacata gagcaaattc aggttcggca tcaaacacat
     4741 gaccattcaa ttggaaaccg agtgctgcga ccccgacgac atccactgcg accttcggaa
     4801 ctgaccgggc accaccacga aacacagacc ttcgcgcacc atcattaagt ggtacagagc
     4861 gctcagactg ccggctcgtt ctcacttcat catcatggtc cttgtcactc agtcctcgca
     4921 cgatccaccc gcacggcctc cacaatctct ttcccactca gcgcagcact gcgctttcgc
     4981 aaacgccgca caacatccgt ggcatg
//
LOCUS       DATMTI010000452         6250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_484488, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000452 DATMTI010000000
VERSION     DATMTI010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484488"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3895
                     /locus_tag="VN039_07330"
     CDS             <1..3895
                     /locus_tag="VN039_07330"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HWV45816.1"
                     /translation="RDLIPPVVRRFTSPHEATPRTHLLSNGRYAVMLTTSGSGYSRWR
                     DIAVTRWREDATRDCWGSYIFLRDIQTGAVWSAGYQPSGVEADDYEVSFTEERTEISR
                     RDGDWNTTLEVVVSSEDDAEVRRISVTNMGTGARELQVTSYAELSLAPQAADVAHPAF
                     ANLFVQTEFVPEVGALLATRRRRSDEETPVWAAQVVVVEGEVIGDLQYETDRARFLGR
                     GHQARTAVSMSDGRPLANTVGSVLDPVMSLRRTVRLPPGATVRVVFSTIVGPARDYVL
                     ELADKYSDPRVFERALSLAWTQARVQLHHLGIENDEAQLFQQLANAVLYSDVSLRPSS
                     ETLSQGTVDRAALWAHGISGDLPIVLACIDKADDVDIVRQLLRAHEYWRMRQLSTDVI
                     ILNEKASSYEQGLQGSLEGLVRGSRLRLCPDAGGARGGIYLLRTDLLSEQDRLLLQQV
                     ARVILLGRRGTLVEQVTRLQRKHRVAPLNLPMRRAATRLDVPVPERELEFFNGLGGFA
                     DDGREYVTILDEGLRTPRPWINVVANPSFGFLVSESGSGFTWSLNSHENQLTPWSNDP
                     VSDPSGEVLYIRDDDTGEVWGPTALPIRDDPAPYVACHGQGYSRFHHGSHGVLAELLQ
                     FVPPEDPIKISRLTLQNMSGRSRRLSVTAYVEWVLGSSRSESAPYIITEIDPGSRALF
                     ARSMLGGEFAGRIAFTDLAGTHTSWTGDRTEFLGRNGTFDRPLALQAGGELSRKVGAG
                     LDPCGALQLSIELDAGERAEIVWFLGQTAGREQARLLINRYRGADVDALLREVTCGWD
                     DRLGGVQVHTPERAMDIFLNRWVLYQTLVCRVWARAAFYQLSGAYGFRDQLQDVMALS
                     VSARDITREHLLRAAGRQFVEGDVQHWWHPPSGRGVRTRISDDLLWLPYAVIQFLEVT
                     GDMTVLDEIVSFLEGPALAEGQHESYFEPQVSQTRATLFEHCARALDRSLVVGSDGLP
                     LMGTGDWNDAMNRVGRQGKGESVWLGWFLHSVLWEFAKVAAQRGERVRAETWRLHVSA
                     LKAAIERNGWDGEWYRRAYFDDGTPLGSAADPECRIDSIAQSWGVISGAADPDRGARA
                     MAAVERYLVNRRDGLIRLLAPPFDQMSRDPGYIKGYVPGVRENGGQYTHAAVWTVLAF
                     AALGEGDKAGELFRMLNPVHRVSSRASVQRYKVEPYVVAGDVYAEPPHVGRGGWTWYS
                     GAAGWLYRAGVEWMLGFRLRGTILSIDPCIPRQWLGYSIRFRYHSAVYDIAVENPRHV
                     SRGVTLAELDGKPLISRDNIALVKDGEHHLRIVLG"
     gene            3950..4408
                     /locus_tag="VN039_07335"
     CDS             3950..4408
                     /locus_tag="VN039_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV45817.1"
                     /translation="MMIEEKNKTTKWEIPGVPAGGLRRVGQIRGTNTLILHAGQNGML
                     VALELLSTHTPGAPVVDDRNEFIGFISEFDVLRALEAGKDLSKLTAEDIMVHDQISVT
                     AETTIEEAVKTMEEKRLHNLPVKKDGKIAYSVTRHDLLRAWIGLGTSGED"
     gene            4758..5255
                     /locus_tag="VN039_07340"
     CDS             4758..5255
                     /locus_tag="VN039_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HWV45818.1"
                     /translation="MSTPHHPRDKKDRSVQARKHDRYKAKGKLQEPSVCRECQAVYHK
                     GRWVWGPIPSDSHEVMCPACERIRDRAPSGVLLLTGEFVAKHRDEVLGLARNEEVRLK
                     AEHPLARIIKIEDQTEAPEGVVITTTDPHLARRIGEALHHAHRGTFACRYEENEDLFR
                     ANWEC"
     gene            5370..5546
                     /locus_tag="VN039_07345"
     CDS             5370..5546
                     /locus_tag="VN039_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45819.1"
                     /translation="MQLTLAPQEQVVLSWAVESAISDLGTEIGHTDNQAMRQDLQERK
                     GILLTILGRLRVTT"
     gene            5804..6205
                     /locus_tag="VN039_07350"
     CDS             5804..6205
                     /locus_tag="VN039_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45820.1"
                     /translation="MFVYLLVFAIVIGFPLSGFAQFVDKGEYVEVAQGTKLCPDVILE
                     KSKGRLDPENVYWRVTSKVRANGTQTVAAFVLETLYDRKSGETTIKERRYDEDYAMLF
                     KRSPEHKGMLLMISPKDGRVEATVEICRKKK"
BASE COUNT         1262 a         1640 c         2013 g         1335 t
ORIGIN      
        1 ccgcgacctc ataccgccgg tcgtgcggcg atttacctcg ccgcatgagg caactccgcg
       61 gacacatctg ctgtccaacg ggcgatatgc ggtcatgctg acgacatccg ggtccggata
      121 cagccggtgg cgggatatcg cagtgacgcg ctggcgcgag gatgcgacgc gggactgctg
      181 gggctcttat atttttctgc gcgatatcca aacaggggct gtatggtcgg ccggttatca
      241 gcccagtggc gtcgaggccg acgactacga ggtgtctttt accgaagagc ggaccgaaat
      301 ttctcggcgg gacggtgact ggaacacgac gcttgaggtc gtggtgtcgt ccgaggacga
      361 tgccgaagtg cggcgcatct ccgtgaccaa tatgggaacc ggcgcgcgcg agctacaagt
      421 gacttcctat gccgagctgt ctctggctcc acaggccgcc gatgtagccc atccggcttt
      481 tgcaaatctg ttcgtgcaaa cggaattcgt gccggaggtc ggcgcattac tcgccacgcg
      541 tcgcaggcgg tcggatgaag aaacgccggt gtgggcggcc caggtggtag tggtcgaagg
      601 cgaagtcata ggcgatctgc aatatgaaac ggatcgtgcc cggtttctcg gacgcggaca
      661 ccaggctcgt acggccgtgt cgatgagcga tgggcggcca cttgcgaata ccgtcggatc
      721 ggtgcttgat ccggtgatga gcttgcgccg cacggtgcgg ctgcctccag gcgcgacggt
      781 gagggtcgtg ttttcgacca ttgtcggccc agcccgcgat tacgtgttgg agttagccga
      841 caaatacagc gacccgagag tatttgagag ggccctgtcg ctggcctgga cacaagcgcg
      901 agtgcaactg catcacctcg gtatcgagaa cgacgaggcg caactattcc agcaactggc
      961 caacgcggtg ctgtactccg atgtctcgct gcggccgtct tccgaaacac tgagccaggg
     1021 cacggtggac cgtgcggcgc tgtgggcgca tggcatctca ggcgatttgc ccatcgtgtt
     1081 ggcctgcatc gacaaagcgg acgatgtcga catcgtccgg caattgctcc gagcgcatga
     1141 atactggcgc atgaggcaat tatcgaccga tgtgatcatc ctgaatgaga aggcctcgtc
     1201 gtatgagcag gggttgcaag gttcgttgga agggcttgtg cgcggcagtc ggctgcgcct
     1261 ctgcccggat gccggcgggg cgcgaggcgg tatttatctt ctgcggaccg acctgctctc
     1321 agaacaggat cggttgctgc tgcagcaggt cgctcgggtc atcctactcg gccgacgtgg
     1381 cacgttggtg gagcaggtta ctcgcttaca gcgtaagcac cgtgtggcgc cgttgaacct
     1441 accaatgcgg cgcgccgcca cacgtctgga cgtgccggtg cctgaacgtg agctcgaatt
     1501 ctttaacggc ttgggcggct tcgcagacga tgggcgcgaa tatgtcacga ttctcgatga
     1561 gggtctgcgg acgccacgac cctggattaa tgtcgttgcg aatccgtcgt tcggattcct
     1621 cgtgtcggag tccggatccg ggtttacttg gtcattgaac agccacgaga atcagctgac
     1681 accctggtcg aacgatccgg tgtctgatcc gtcgggcgag gtgctctata tccgtgatga
     1741 cgacaccgga gaggtctggg gtccgacggc gctgccgatt cgcgacgatc cggcaccgta
     1801 cgtcgcctgt cacggacagg gctatagtcg cttccatcat gggtcgcacg gtgttttggc
     1861 cgaattgctg caatttgtac cgcctgaaga tccgatcaag atctctcgat tgacactgca
     1921 gaatatgtcc ggccgatcga gacgcctttc agtcacggcc tatgttgagt gggtgctcgg
     1981 gagttctcgc agtgagtccg caccgtacat catcacggag atcgatccgg ggagcagggc
     2041 gctttttgcg cgcagcatgc tgggcggcga gttcgcagga cgcatcgcgt ttaccgatct
     2101 tgccggaacc catacgtcgt ggaccggtga tcgtacggaa tttctcggtc gtaacgggac
     2161 tttcgatcgt cccttggcgc tgcaagcagg aggggaattg tctcgaaagg tcggcgcggg
     2221 tctcgatcct tgtggggccc ttcaactgtc gattgagttg gacgcaggcg aacgcgccga
     2281 aatcgtctgg tttcttggtc agacagcggg gagagaacag gctcggctcc tgattaaccg
     2341 ttaccgtggg gcggacgtag atgcgcttct gcgcgaggtg acttgcggat gggacgaccg
     2401 attgggtggc gtgcaggttc atacgccgga gcgcgctatg gatatcttct tgaatcgctg
     2461 ggtgctctat cagacgctgg tttgccgggt ctgggcacgt gcggcctttt accagttgag
     2521 cggggcgtat gggtttcgtg atcaacttca agatgtcatg gccctgagcg tatcggcacg
     2581 cgatatcaca cgtgagcatc tgctgcgggc tgctgggcgt cagttcgtcg agggggacgt
     2641 ccaacattgg tggcatcctc cgtccggacg cggtgtgcgg actcgcattt ccgatgatct
     2701 actctggttg ccttacgcgg tgatccaatt tcttgaagtg accggcgaca tgactgtgct
     2761 ggacgaaata gtgtcctttc tggaaggccc ggcattggct gaagggcaac atgaatccta
     2821 ttttgaacca caggtgtccc agacacgcgc taccctcttt gaacattgtg cgcgtgcgtt
     2881 ggaccggagc cttgtcgtcg gcagtgatgg ccttccgctc atgggtaccg gagactggaa
     2941 tgacgcgatg aaccgagtcg gccggcaggg caaaggggaa agcgtctggc tcggctggtt
     3001 tctgcattcg gtgctctggg agttcgccaa ggtggcggct cagcgtggtg agcgtgttcg
     3061 tgctgaaacg tggcggctcc atgtgagtgc gttgaaggct gcgatcgagc ggaacgggtg
     3121 ggacggggaa tggtaccgcc gcgcgtactt cgatgatggg acgccactcg gttccgctgc
     3181 agatccggaa tgccgcatcg attctattgc ccagtcctgg ggagtgatca gcggcgcggc
     3241 agatccggac cgtggcgcac gcgccatggc cgcggtggaa cggtatcttg tgaatcggcg
     3301 agacgggcta atccgtctgc tggcaccacc ctttgatcaa atgtcgcggg atcctggcta
     3361 tatcaaaggc tatgtccctg gcgtgcgaga aaacggcggt caatacaccc acgcggcggt
     3421 ctggaccgtg ctggccttcg cggcgttggg tgagggtgac aaggccggcg agttattccg
     3481 catgttgaat cctgttcatc gggtctcctc gcgagcgagc gtccagcgct acaaagtcga
     3541 gccctacgtc gtagccggcg acgtgtatgc cgagccgccg catgttggac gcgggggatg
     3601 gacttggtac agcggcgcgg cgggttggct ctatcgggcc ggtgtggaat ggatgctcgg
     3661 tttccgcttg cgtggtacca tactctccat tgacccttgt attccgcgcc aatggctggg
     3721 gtactcgatt cgtttccggt atcattcggc ggtctatgac atcgcggtgg aaaatccgcg
     3781 ccatgtgagt cgcggcgtga cgctggcgga gcttgatggc aagccattga tcagccgcga
     3841 caatattgcc ctcgtcaaag acggtgaaca ccaccttcgc atcgtgctcg gttgatacgg
     3901 gggtgggata atcgccaatc ctatgttgta gagcaaccta aaggggggaa tgatgattga
     3961 agaaaagaat aagacgacaa agtgggaaat acccggggtt ccggccggag gattacgaag
     4021 ggtggggcag atcagaggga ccaatactct cattttacac gccggccaaa atgggatgct
     4081 cgtggctttg gaactactgt ccactcatac tcccggcgcg ccggtggtcg atgatcgcaa
     4141 cgagttcatc ggattcatca gcgaatttga tgttctccgc gcgttggaag ccggcaagga
     4201 tctttcgaag ctgaccgcgg aagacatcat ggtccacgat caaatttccg tcacggccga
     4261 gaccacgatc gaagaagcgg tgaagacgat ggaggagaag cgcctgcata atcttcctgt
     4321 gaagaaggat gggaagattg cctactctgt gacgcggcat gaccttctgc gagcctggat
     4381 tggcctcggc acttcgggcg aagattgaga agagtcgcct tcgcgatggg cggaggggca
     4441 atcgaaagcg gagtgcgctg gggcttagag tggggcatgg cagaaactta gtcgtatggg
     4501 ggacgaggat gtgaaggatc gttcgtaggc acaggcgatc gcacagcaga gcacggactc
     4561 tcctcgtcgt ctcatggttt ccacggattt gagcatgtcg cacatccgtt gcagaatggc
     4621 attccacatg cagattcacg attggtatga caatgaggac ttacgaaacc ggcctgtctg
     4681 aatcaggaac gatacgctgg gggatctctg tctagccgga ttcggcggat atctctcacg
     4741 cgatggagtg atggaacatg tcgacaccac atcatcctcg agacaagaaa gatcgatcgg
     4801 ttcaagctcg aaagcacgac cggtacaaag ccaagggcaa acttcaggag ccgtcggtgt
     4861 gtcgtgagtg ccaggcggtg tatcacaagg ggcggtgggt gtggggccct atcccatcgg
     4921 acagtcacga ggtgatgtgt cctgcctgtg aacggatccg ggaccgtgcg ccgagcgggg
     4981 tgctgctgct gaccggtgag tttgtcgcca aacatcggga cgaggtgctt gggctcgccc
     5041 gcaatgaaga agttcgtctc aaagccgaac atcctctagc tcgaatcatc aagattgagg
     5101 accaaaccga agcacccgaa ggggtggtga tcaccacgac cgatccccat cttgcgcggc
     5161 gcatcggcga ggcgttgcat cacgcccatc gtggcacatt cgcctgtcgt tatgaggaga
     5221 acgaggatct gttccgggcg aactgggagt gttaagcgga gggacaagag tcggaatctc
     5281 cgccgatcga atggggcgaa cgcgccaccg gattttctac tttccgggcg atgcccgctc
     5341 aaggagtgca gctcaagaag gaggatcata tgcagctgac tttggcacct caggagcagg
     5401 tggtattgtc ctgggcggtc gagagtgcca tctcggacct cggtaccgag atcggtcaca
     5461 ccgacaacca ggccatgcgg caagacttgc aagagcgaaa gggaatcctc ttaacgattc
     5521 ttggccggct cagagtcact acctgagagg gtctcctctc cgcacgcgag cgttctagac
     5581 ttcaggatga ggagatgctg cagtgatgaa ccgcgtaccg tcgtccctcg gcaatgatcg
     5641 atccagaagg taagcggaac gtccgcccgt gatcgattgt ccggtggatt atagaaaaat
     5701 cttcgaatga ccgagtgtct gaagcgtgtg tcttaggccg ttctctttaa gcctgtctgg
     5761 atcgcgtaca atgccttcac gccctttcat gaaggagaat cacatgttcg tgtacctgct
     5821 ggtttttgca atcgtcatcg gattcccttt gtcaggcttc gctcaattcg tggataaagg
     5881 cgagtatgtc gaggtggcgc aaggcacgaa gctttgtcct gacgtcatcc tggaaaaatc
     5941 gaaagggagg cttgatccag agaacgtcta ttggcgagtc accagtaagg tacgagcgaa
     6001 tggcacacag accgtcgccg catttgtcct tgaaaccctg tacgaccgca aaagcggtga
     6061 gaccacgatc aaggaacgtc gatatgacga ggactatgcc atgctcttta agagatcccc
     6121 ggagcacaaa gggatgcttc tcatgatcag tccgaaagac ggtcgagtgg aagcgacggt
     6181 ggagatttgc aggaagaaga aatagcgtgt ctgccctcga cgcctcttat cgatcagagc
     6241 gctccgtaca
//
LOCUS       DATMTI010000453         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_271470, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000453 DATMTI010000000
VERSION     DATMTI010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271470"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /gene="pheA"
                     /locus_tag="VN039_07355"
     CDS             <1..920
                     /gene="pheA"
                     /locus_tag="VN039_07355"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWV45821.1"
                     /translation="EIIERLTKLSPGPFPSEAIRSVYREIMSASLSLESPQKVAYLGP
                     RATFTHMACMQKFGSSVQYVPVHSIKEVFSEVERSRANFGVVPIENTTEGVVNHTLDM
                     FIDSNLLIYGEILQEVSHHLLSKSGLIEDVKKIHSHPHALAQCRNWLETNLPHVPAVE
                     VASTARAAELCIDEPPSAAIASELAGQLYGLKVIKGRIEDNLNNFTRFLILSQKPSER
                     TGKDKTSLMLSVKDKVGALYDLLRPFASHGINMTKIESRPSQRKAWEYIFFVDVEGHI
                     HEERVNRAVEEVKGRCLFMKILGSYPIHS"
     gene            923..2008
                     /gene="hisC"
                     /locus_tag="VN039_07360"
     CDS             923..2008
                     /gene="hisC"
                     /locus_tag="VN039_07360"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWV45822.1"
                     /translation="MALQVHPDILSLSPYIPGKPIDELQRELGLSRVIKLASNENPLG
                     PSPKALAALSGAQDMLHRYPDGSAYRLRQAIADRWKVAEGQVILGNGSDEILGLLAKT
                     FLTPGDKAIMADHTFVIYKMEVTAVHGKPVVVPLVNWTHDLESMVRAVTPRTRLLFLC
                     NPNNPTGTIVSAEAVDRLMAKVPEDVIVVFDEAYFEYVRNPRFPDAMAYVKQRRNAIV
                     LRTFSKIYGLAGLRIGYGISTEEIIDYLNRVRPPFNANSLAQKAALAALGDDEHVAKS
                     RTVNASGMEQIEEGLRVLGVTSIPSETNFLYFDAKRNGRLVFEALLREGIIVRHIDGT
                     MMRVTIGQPDENAAFLQALKKVLDGSR"
     gene            2026..>2890
                     /gene="aroF"
                     /locus_tag="VN039_07365"
     CDS             2026..>2890
                     /gene="aroF"
                     /locus_tag="VN039_07365"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWV45823.1"
                     /translation="MIIVLKPEASESEVDHIIDRLRDLGLKSQISTGQERTIIGVIGD
                     DRILYNQPLTALPGVESVLPILAPWKLVSREFKKEATIIDVGGAKIGAKKLAIMAGPC
                     AVERLELTVGIAHEVKAAGASVLRGGAYKPRTSPYSFQGLGREGLDYLLEAKKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNQNVMLCERGIRTFETQYRNTLDLAAIPTLKELSHLPVIVDPSHATGK
                     WDLVAPMSKAAV"
BASE COUNT          708 a          741 c          788 g          653 t
ORIGIN      
        1 ctgagatcat cgaacggctc acgaaactca gcccaggccc tttccccagc gaggctatcc
       61 gatcggtcta tcgagaaatc atgtcggctt ccttgtcgct cgagtctccg cagaaggtag
      121 cgtatctggg acctcgggcc acctttaccc acatggcctg catgcagaaa ttcgggtcgt
      181 ccgtccagta tgtgcctgtt cacagtatca aagaggtatt cagcgaagtt gaacgaagcc
      241 gagccaattt cggggtggta ccgattgaaa acaccaccga gggtgtcgtg aatcacactc
      301 tcgacatgtt tatcgattcc aacttgctga tctatggaga gattcttcaa gaggtctcac
      361 atcatctctt gtcaaaatcg ggacttatcg aagacgtaaa gaaaattcac tctcatcctc
      421 atgctcttgc gcaatgccgg aactggctag agaccaatct tccgcatgtc cccgcagttg
      481 aggtagccag cacggctcgt gccgccgaac tgtgcatcga tgaaccacct tcggctgcga
      541 ttgcgtcgga attggccggg caactgtacg ggctcaaagt aattaagggc cgtatcgaag
      601 ataatctcaa caattttacg cgtttcctga ttctgtcgca gaagccgtcg gagcgcacag
      661 ggaaagataa aacgtcgttg atgttgtcgg tgaaggataa agtcggcgca ctgtatgatc
      721 tgcttcgtcc ctttgcctct cacgggatca acatgaccaa gatcgaatct cgcccttccc
      781 agcgaaaggc gtgggaatac atttttttcg tggatgtcga aggccatatc catgaggagc
      841 gggtcaatag agcagtggaa gaagtaaagg gccgctgcct cttcatgaag attttgggct
      901 cctacccgat tcatagttga ccatggcact acaggttcac ccagatattc tgtcactcag
      961 tccatatatc cccggtaaac cgatcgacga gctacagcga gagctcggcc tctcccgtgt
     1021 cataaagctt gcctccaacg aaaatccgct tgggccttca ccgaaagcgt tggcagcact
     1081 gagcggcgca caggatatgc tgcatcgata tccggacgga agtgcgtatc ggctcaggca
     1141 ggcgatcgct gatcgttgga aagtggcgga gggacaggtc attcttggaa atggatcaga
     1201 tgagattcta ggcttgttgg ccaagacgtt cttgactccc ggtgataaag ccatcatggc
     1261 cgatcacacg ttcgtcatct ataagatgga ggtcactgct gttcacggca agccggtggt
     1321 cgtaccgctc gtcaactgga cacacgatct cgagtcaatg gtacgtgccg ttacacctcg
     1381 aaccagattg ctctttctct gcaacccaaa taatccgaca gggaccatcg tatcagcgga
     1441 agcggtcgat cgcctcatgg caaaagtgcc ggaagatgtc attgtcgtgt ttgacgaagc
     1501 gtactttgag tatgtccgca atccccgatt tcccgatgcg atggcatatg taaaacagag
     1561 gcggaacgcg atcgttttaa ggacattttc caagatctat gggctggcgg gattgcgaat
     1621 cggatacggc atcagcacag aggagattat cgactacttg aatagagttc ggcctccgtt
     1681 caatgccaac agccttgccc agaaagccgc attagccgca ttgggagatg acgaacatgt
     1741 ggccaagagt cggacggtta atgcgtctgg aatggaacag atcgaagagg ggctgcgcgt
     1801 gttgggcgtg acttctattc cgagcgagac gaattttctc tattttgatg cgaaacggaa
     1861 tgggcgactg gtgttcgaag cattgctgcg agaaggaatt attgtacggc atatcgatgg
     1921 cactatgatg cgtgtcacca tcggccagcc tgatgaaaat gctgccttcc tccaagctct
     1981 caagaaagtc ttggatggaa gtcggtaaga tatagtgagg gcattatgat catcgtgttg
     2041 aaacctgaag cctccgaaag cgaggtggac cacattatag accgactgag agatctgggt
     2101 ttaaaatcgc aaatctccac cggtcaggaa cgcacgatca tcggcgttat cggagacgac
     2161 cggatcttgt acaatcagcc cttgacggcg cttcccggcg tggaaagtgt cctgccgatc
     2221 cttgcgccct ggaaactggt cagtcgtgag tttaagaagg aagcgaccat catcgacgtc
     2281 ggtggtgcaa aaatcggtgc caagaagttg gccatcatgg ccgggccctg tgcggtcgaa
     2341 cggcttgagc tcacggtagg gatcgcacat gaggtgaagg ccgccggagc ctcggttcta
     2401 cgtggcggag cctacaagcc aagaacatcg ccgtattcct ttcaagggtt gggtcgtgaa
     2461 gggcttgact acttgcttga agcaaaaaaa caaaccgggc taccggtcgt cagtgaaatc
     2521 ttggacacca gagacatcga actctttctc gagaaggcgg acatcatcca gatcggcgct
     2581 cggaacatgc agaacttcga actcctgaag gaggtcggcg cctacgacaa gccggtgctc
     2641 ctgaaacgag gcctgtcggc aacgatcaaa gagtttctcc tgtcggctga gtacatcatg
     2701 tcacgcggaa accagaacgt catgctgtgc gagcgtggca ttcgcacgtt cgaaacgcaa
     2761 tatcgcaata cgctggatct tgcggccatt cccacgttga aagagctttc acatctgccg
     2821 gtcatcgtcg accccagcca tgccaccggg aagtgggacc ttgtcgctcc gatgtccaaa
     2881 gctgccgtgg
//
LOCUS       DATMTI010000454         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_230866, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000454 DATMTI010000000
VERSION     DATMTI010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230866"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1078
                     /locus_tag="VN039_07370"
     CDS             308..1078
                     /locus_tag="VN039_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWV45824.1"
                     /translation="MNRGIYPILSGALAHERRMQVFANNMANANTAGFKQDEQAFKTI
                     FPRYHAVIPVGSPTMGLAQQIMARPLGVTERAFVAPHTMKTTFEVGRIRLTGNALDIA
                     IQGPGFFEVNTPQGTRYSRNGMFSLDRERRLVNGLGYPVMGTKGEIKIPPGKLEITTQ
                     GDIKVDDKSVATIKVMEFHEGEMPQKSSDGLFSSDKGKLAKTPQLQVGHIEESNVNSI
                     GEMVKMIQGMRNYESAQKLIQTLDHMADVAIQDVGRVL"
     gene            1194..>1664
                     /locus_tag="VN039_07375"
     CDS             1194..>1664
                     /locus_tag="VN039_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWV45825.1"
                     /translation="MIRAMWTAATGMTAQQINVDTIANNLANVNTNSFKRSRAEFADL
                     LYQIQRLPGTSASTIGVFPVGVQVGAGVRPTTVAKEWLQGNMRQTNNDLDLAVDGVGF
                     FQVTRPDGTIMYTRNGAFKRDNLGNVVTGDGDLLNPVITIPSGALKVDIGMDGTV"
BASE COUNT          411 a          427 c          437 g          389 t
ORIGIN      
        1 ctatgatcct cacgaccagt cgtcgatgat tcgaactgtt ctgcttccgt gctctcctgg
       61 cgcgttgttt tgtaaaccca ctaggaatat ttttctctgg gcctggtagg aatggcctag
      121 cgctatcctc ccaagcaatg gcgaggtccc tcattcttta ttctatgggc attcagtccc
      181 ctcagtctct taaggaattg gctcatttcc aatctcccct tagtacttat ccgatcagta
      241 tctcgtcggg cgaacctggc atgagggttg ctgctcttcc tttctcgaaa gcaaaggagt
      301 cggcagcatg aatcgaggaa tctatcctat cttgtcagga gcactggccc atgagcggcg
      361 gatgcaagta tttgccaata acatggcaaa cgcgaatacc gccggcttca agcaggacga
      421 gcaggccttc aaaaccatct ttcctcgata ccatgcagtg attccggtcg gcagtccaac
      481 catgggattg gcccaacaga tcatggccag acccttaggg gttacggaac gtgcgtttgt
      541 ggccccacac acgatgaaga cgacatttga agtgggtcgg atcagactca caggcaatgc
      601 cttggacatt gccattcaag gccccggatt ttttgaagtg aacactccac aaggaactcg
      661 ctacagccgc aacggtatgt tttcgctcga tcgtgagcgt cggttagtga acggactcgg
      721 ctatccggtg atggggacga agggtgagat caagatccct cccggtaaat tggagattac
      781 gacacaggga gacatcaaag tcgatgataa atctgtggcg acgatcaaag tcatggaatt
      841 tcacgaaggc gagatgccgc aaaaatcatc ggatggttta ttttcctccg acaaaggcaa
      901 actcgccaaa accccacagc ttcaggtcgg acacatcgaa gagtccaacg tcaattccat
      961 cggcgagatg gtgaaaatga ttcagggcat gcgcaactat gaatcggcgc aaaagctgat
     1021 tcagacgctt gatcatatgg ctgatgtcgc aattcaagac gtagggcggg tgctatgaga
     1081 aggaatgtgt atcgcagacg gccactaagt ggtggcacgg cctggatatc atctgatgcg
     1141 aaatctcttg ttctcagcta tctgcgatcg gttcttcagc tctggaggat tctatgatcc
     1201 gtgccatgtg gacagcggcg actggaatga cggctcagca gattaatgtc gacaccatcg
     1261 ccaataacct tgccaacgtc aataccaact ctttcaaacg cagccgcgct gagtttgctg
     1321 acttgctcta tcaaattcaa cggcttcccg gcacgagcgc atccaccatc ggagtctttc
     1381 cagtcggcgt tcaggtaggc gccggggtgc gtccaacgac agtggcaaaa gaatggctgc
     1441 aaggcaatat gcggcaaacg aacaacgact tggacctggc cgtcgatgga gtcgggttct
     1501 ttcaagtaac tcgtcccgac gggacgatca tgtatacaag aaacggggca tttaagcgcg
     1561 acaatttggg caatgtcgtg actggcgatg gcgatctcct gaatcccgtg attacgatac
     1621 cctccggtgc actgaaggtg gatatcggta tggacggcac cgtc
//
LOCUS       DATMTI010000455         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_484551, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000455 DATMTI010000000
VERSION     DATMTI010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484551"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..533)
                     /locus_tag="VN039_07380"
     CDS             complement(30..533)
                     /locus_tag="VN039_07380"
                     /inference="COORDINATES: protein motif:HMM:NF023170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HWV45826.1"
                     /translation="MKREAKNVRIQAIAMAPRVYCGWVAALSAFAVMALPTQAHASLF
                     HGETLDSIANGISWVVLIVAPIIGIAAFWLVHILPEKIAHKRKHPQTKAIQCLCLLSL
                     CFGGLLWPIAWLWAYTKPVLHKMAYGTDVDESLSHVGHEVTEEDKEIEKLRTRIAELE
                     AKRSGVS"
     gene            complement(683..1123)
                     /locus_tag="VN039_07385"
     CDS             complement(683..1123)
                     /locus_tag="VN039_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWV45827.1"
                     /translation="MNETNSLPQEEIVWRGTSSQWKNFGVYVLCGLLCWLIVPIVFAL
                     IYYFKTKCKVFELTTQRLKITSGVFTKVTETLELYRVKDIETRQPFFSRLVGIENVQM
                     TTTDVSSPIVLIEAVPSSVGFADKLRHQVEIIRQQKRVREIDIE"
     gene            complement(1197..>1501)
                     /locus_tag="VN039_07390"
     CDS             complement(1197..>1501)
                     /locus_tag="VN039_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HWV45828.1"
                     /translation="QKQRFLDRIDDPLKNWKFSSNDASERDYWDDYMKAYEEMIQETS
                     TKYAPWYVVPADNKWFTRVIVASAVIETLDSLDLAYPEVDESKLKELAAAKKKLLS"
BASE COUNT          340 a          397 c          375 g          389 t
ORIGIN      
        1 atgatcagga gaatgagttc cattacgcct taactcactc cgctgcgttt cgcttcgagt
       61 tccgcaattc gagttcggag tttttcgatt tctttgtcct cctccgtcac ctcgtggcca
      121 acgtggctga gcgattcgtc cacatcggta ccgtaagcca ttttgtgcag gaccggcttg
      181 gtgtaagccc aaagccacgc aattggccag agcagcccgc caaagcacag cgagagcagg
      241 cacaggcatt gaatcgcttt cgtctgtgga tgttttctct tatgggcaat tttttccggc
      301 agaatgtgaa caagccagaa agcggcgatg ccaataatgg gcgcaacgat gagcacgacc
      361 caggagatgc cgttggcgat ggagtcgagc gtttctccgt ggaaaagcga ggcatgagcc
      421 tgagttggca acgccattac ggcaaacgcg ctcaacgcag cgacccaccc gcagtaaacg
      481 cgtggcgcca ttgcgatggc ctgaatcctt acatttttcg cttcacgctt cattctcttt
      541 gatccgctct ataagatgcc gatttcgttt tgtcgagact cgaagcgcca agctcattcc
      601 ccgtttctac tcggcgacca atttggttgg aagcggtccg gttgcgtatt ggactttaga
      661 cctacgtcgg taaagcagat ctctactcga tgtcgatctc acgcactcgc ttttgctggc
      721 gaatgatctc gacctgatgg cgtagcttgt ccgcgaatcc aacgctcgac ggaacagctt
      781 cgatcagaac aatcggcgaa gacacgtctg tcgtggtcat ctggacattc tcaatgccga
      841 ccagtcgtga gaaaaacggt tgccgggttt cgatgtcttt tacccgatac aattcgagcg
      901 tctcggtgac tttcgtaaac acgcctgaag taatttttaa gcgctgagtc gttagctcga
      961 acactttgca tttcgttttg aaataataaa tcagcgcgaa aacgattggt acgatgagcc
     1021 agcaaagaag cccgcataag acatagacac caaagttttt ccactgcgag gatgtgccgc
     1081 gccacacaat ctcttcctgc ggcaatgagt tggtctcgtt catcggatgt ccttagtaat
     1141 gagctccgtt cacagcaagg cgggctaaaa gggcgcgtcc acaatggcga gcgctattag
     1201 ctcaacaatt tctttttcgc cgccgccaac tccttgagtt tcgattcgtc cacctctgga
     1261 taagcaagat cgagtgagtc gagagtttcg atgactgccg acgcgacgat gacgcgcgta
     1321 aaccatttgt tatccgctgg cacgacatac cagggcgcgt acttcgttga ggtttcctgg
     1381 atcatctctt cgtacgcttt catgtaatca tcccaatagt cacgttcact ggcgtcattg
     1441 gacgagaatt tccagttttt cagcggatcg tcgattcgat cgaggaacct ttgcttctgt
     1501 t
//
LOCUS       DATMTI010000456         1944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_569122, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000456 DATMTI010000000
VERSION     DATMTI010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569122"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1626)
                     /gene="fliF"
                     /locus_tag="VN039_07395"
     CDS             complement(61..1626)
                     /gene="fliF"
                     /locus_tag="VN039_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HWV45829.1"
                     /translation="MFSKFSVNQRMIILIALAGSVAGLIALALWTQQPDMQVLFTNLS
                     SEDAAAIIDKLKETKVPYETTGGGSTVLVPSAQVHDLRLQLATQGLPHGGGVGFEIFD
                     RTSIGMSEFVQKLNYRRALQGELARTIAQLPEVERARVHLAIPERRLFAHEQEKARAS
                     VIVSLRNGQQLTQSQVQGVIHLVSSSVEGLQARDVTVVDGHGRMLSATMTDETSGLTN
                     SQLDYQHSIEKDVETRIRTMLERIVGTNKAVVRVSSVVNFRKVETTEERYDPNSQVVR
                     SEQRGQEKANGTNGVGGGVPGVQSNVPPGTDQEPAQTSSSNSQTKNETVNYEISRTVS
                     KIVEPVGVIKQLSVAVLVDGTYETPKAGDGQSADAPAAARKYIPRSEEDLKRIEEIVK
                     KAMGYSDERQDQVQVVNVQFGTESEEPQSVTAEAVTEGPKPWLPYLRYAIGILLFTVI
                     LLFVVRPLLTMLGSTAAQTPAESSISAMPGAVGTAEASLAGGPDRTQIIDMARKNPDT
                     TAVVVKQWLKNPT"
     gene            complement(1659..>1944)
                     /locus_tag="VN039_07400"
     CDS             complement(1659..>1944)
                     /locus_tag="VN039_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45830.1"
                     /translation="AKSSSGRVGEKEYASPLRHWALTDSRPSADVTLIILRVADLAAA
                     LPVEKRVLARRGRAGEISSLFEHPEVILALAPYGRFQPYYSHQPSSSAVS"
BASE COUNT          372 a          604 c          521 g          447 t
ORIGIN      
        1 ggcacgaaga agaatggccg ccttctgttc acccgtcaga ttcttcgcca tggcgatatg
       61 ctatgtgggg ttttttagcc actgcttcac gaccacagcc gtcgtgtcgg gattctttct
      121 cgccatgtca atgatctgcg tccggtccgg tccgccggcc aatgaggcct ccgccgtgcc
      181 aaccgcgccg ggcatcgcag atatcgatga ctcggccggc gtctgcgccg ccgtcgaacc
      241 caacatggtc aacaacggac ggacaacgaa caagagaatc actgtaaaca aaaggattcc
      301 gatcgcatag cggagatagg gcagccatgg cttcggaccc tccgttacgg cttctgcggt
      361 cacactctgg ggctcttccg attccgtccc gaattgaacg ttgacgactt gcacttgatc
      421 ttgccgctcg tccgaatagc ccatcgcttt cttgacgatc tcctcgatcc ttttcagatc
      481 ttcctccgaa cgtggaatgt atttgcgtgc ggcagccggg gcatccgcag actgtccgtc
      541 accggctttg ggcgtttcat aggttccatc gaccagcaca gccacggaca attgtttaat
      601 gacgcccacc ggctcgacga tttttgaaac ggttcgacta atctcgtagt tgaccgtctc
      661 atttttcgtc tgactgttgc tcgaactggt ttgcgctggt tcttgatccg ttcccggtgg
      721 gacgttcgac tgcacgcccg gcacgccgcc cccgacaccg ttggtgccgt tggccttttc
      781 ctgcccccgc tgttcgctcc tcacgacctg gctgttcgga tcataccgct cctccgtcgt
      841 ctcaaccttt cgaaaattca ccacgctgga gacacgcacc acggctttat tggttccgac
      901 gatccgctcc aacatcgttc ggatacgcgt ttccacgtct ttttcgatgc tgtgctgata
      961 gtcgagctga gaattggtca aaccggaggt ttcatccgtc atcgtggccg acaacatacg
     1021 gccgtgccca tccaccaccg tcacatcgcg agcctgcagt ccttccacac tgctcgacac
     1081 cagatggatg accccttgca cctgcgactg agtcaactgt tgtccgttgc gaagcgacac
     1141 gatcacggac gcccgcgctt tctcctgttc gtgggcgaag aggcgccgtt ccggaatcgc
     1201 cagatgaacc cgtgctcgtt cgacttccgg cagctgcgca atggttctcg ccaactcgcc
     1261 ctgtaaggca cgacgatagt tgagtttctg gacgaattcg gacatgccga tcgaggtacg
     1321 atcaaagatc tcgaacccga cccctcctcc gtggggaagc ccttgggtcg ccagttgcag
     1381 tcgtaggtca tggacttggg cgctcgggac caacaccgtc gatccaccgc cggttgtttc
     1441 gtacggcacc ttcgtctctt tgagcttatc gatgatggcg gcggcgtctt cgctgctgag
     1501 attcgtgaac aagacctgca tgtcgggttg ctgggtccat agcgcgagag cgatgaggcc
     1561 cgccaccgat ccggccagag cgatgaggat gatcatccgc tgattgacgg agaactttga
     1621 aaacattcta gccgctcctc ttcaccgcgc ctgaccgatt aggagactgc agaagaactg
     1681 ggttgatgtg agtagtatgg ttgaaatctt ccgtacggtg ctaatgccag aataacctca
     1741 ggatgctcaa aaaggctgct gatctcaccc gcccgacccc ggcgcgccaa gacgcgcttt
     1801 tccacaggca aggccgcagc gaggtccgcg acgcgaagaa taatgagcgt cacgtctgcg
     1861 gacgggcgcg agtcggtgag cgcccagtgt cttaaaggcg aggcgtattc cttttcaccc
     1921 acccgccccg agctgctctt agca
//
LOCUS       DATMTI010000457         1922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_654123, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000457 DATMTI010000000
VERSION     DATMTI010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654123"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1205
                     /locus_tag="VN039_07405"
     CDS             147..1205
                     /locus_tag="VN039_07405"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HWV45831.1"
                     /translation="MMKAQCQLRLSRTHIKACLVLKTIVLACLWTSFLDTAFSGVRTA
                     EEEQNFQRDREAIRALAGDYHVQFSFRETVSFSKEYRLAAPYEPDGYEIIRVIRDDGA
                     LISLQHILVGHDLWDEPGPTKHWRQDWIFEPEQLFEYVGRDTWHIRRLDDSERRGKWA
                     QLIYQVDDSPRYAALAEWTHAEGVSSWTSPPTWRPLPRREVTKRRDYDVLVSSNRHAL
                     TPNGWVHEQDNSKLILGDRPRLLVREHGVNTYATSQAFDIQVAERYWKDTHAYWDEVR
                     AVWRDLLSRPGSLNVQRGTESGRLDTRLLTLADDVRHTRLDVQTAIARARDIIRASTT
                     SHAVKFDDQDIDTKRRLE"
BASE COUNT          451 a          452 c          594 g          425 t
ORIGIN      
        1 aaataaatgc ttgcgcggcg gtgcaagatc ccgccgttca ctaacggtca cggggacgag
       61 gccagaggtc gttgaagatc ggagcccaag gtgtcggaag acgccttggg agtttttgtt
      121 gaggccgatg aatttggagg aggtccatga tgaaggcgca atgtcaactc aggctgagcc
      181 gcacacatat caaagcctgt cttgtgctga aaacgatcgt gcttgcgtgc ttgtggacat
      241 cgttccttga tactgctttc tcgggagttc ggacggcgga agaagagcag aattttcaac
      301 gtgaccggga agccatccgt gcacttgctg gtgactacca tgtgcagttc agtttcaggg
      361 aaacggtttc attctccaag gaatataggc ttgccgcccc ctatgaaccg gacggctacg
      421 aaatcattcg tgtgattcgt gatgatggcg cgctcattag tttacagcac attctcgtgg
      481 ggcacgatct gtgggacgag cccggcccga cgaaacattg gcggcaggat tggatttttg
      541 aaccggagca gcttttcgaa tacgtcggcc gcgacacctg gcatatccgc cggctggacg
      601 attcagaacg tcgggggaaa tgggcgcaac ttatttatca agtggatgat agtccgcgtt
      661 atgcagcgct cgcagagtgg acgcatgcgg agggcgtatc gtcctggacc tcgccaccga
      721 cttggcgacc tctcccgcgt cgtgaagtca ccaaacgtcg cgactacgac gtgctggtgt
      781 cctcgaatcg tcacgcgctg acaccgaacg gatgggtgca tgagcaggac aattccaagc
      841 tcatcctcgg cgatcgtccg cgattgctgg ttcgagagca tggtgtgaat acctatgcaa
      901 cctcgcaggc cttcgacatt caggttgctg agcggtattg gaaggacacg cacgcctatt
      961 gggatgaagt ccgcgccgta tggagggact tgctcagtcg acccggttca ttgaatgtgc
     1021 aacgtggaac tgaatcggga cggcttgata cgaggcttct aacgttggcc gacgacgtga
     1081 gacatacacg cctcgatgtg caaaccgcaa tcgcgcgtgc ccgcgatatc atccgggcat
     1141 cgacgacaag ccatgcggtc aagtttgatg atcaggacat tgatacaaag agaagattgg
     1201 agtagagggc actgatcgga gggcgaggac acagaacatc atgtgagaat tgtcaaagga
     1261 gggaagcatg aaatcaatct gcggaaaatg cgggggcttc gtggtccagg agcaagtgct
     1321 cgattactat caagcgagtc gtaggaggtg cgtgaactgc ggttggtgtc acagagagat
     1381 tgaagtatcg tccagtcgag ccgtcagatt gatgaatcgt ggcgcctgca gataataccg
     1441 accattgatc gacgaaaggg gaaagcactg tgaaaagtat gttatcaacc gatggggaga
     1501 tgaagctggt cctttgcggg tgcggcaagc tccatgtaac gttaggttcc atcacgctcc
     1561 acttcacgcg tgacgagttt cagctgtttg ccgaaacaat acggcggctt gcggcgatcg
     1621 tggcacaacc atcgttgggg caagcttcgt tgacaccacg atccagttcg agtgaggtct
     1681 gtcactaggg ggtgagaggc atcttgccgg gttgagctgg tcactattga gacaggggca
     1741 gatggaagat tgggcatgag taaacggtac gtgcactctc gagatgatct tgccatagtc
     1801 atcagtttgg cagcggcggt cggcatcctc gtgtcagctg ctgtggcgat gtgggtctgg
     1861 tcaagtttct cggagaacat ggtaacgttc taggaataag gagttgacaa cagcaaccat
     1921 cg
//
LOCUS       DATMTI010000458         1889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_231148, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000458 DATMTI010000000
VERSION     DATMTI010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231148"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /gene="waaF"
                     /locus_tag="VN039_07410"
     CDS             complement(<1..1270)
                     /gene="waaF"
                     /locus_tag="VN039_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWV45832.1"
                     /translation="MGSELVRRNLPRRILVRAPNWIGDAVMCEPAVGGLRSLFPEAEL
                     TMLAKPAIAELFIAHPALHCMLVYEDKGAHAGISGKWSLAGTLRRHRFDLAVLFQNAF
                     EAAFLAWLAGIPRRYGYATDGRVFLLTEPVAVPKDPVPGHQVEYYWNLLRPLGLADGP
                     TLPTLLVSADEDRMADVRLASAGIDSSDLIIGINPGSTYGSAKRWMSDRFAEVAKRLV
                     RRVEQSESVHVAVVILGAKGEEWLGENIAAQLGGRSVVLSGATTIRELMAVLKRCRLL
                     ITNDTGPMHIAAAFGVPVVAVFGPTDWRTTAPYGQERSIVREAVDCAPCLFRECPIDH
                     RCMTRVSVERVYEAGLSRLSGPSGLSGPDKTDQIDRTDQTDKIDQTDRKILSGYTIFL
                     DRDGTLNPDPGYINSSDQFELFPGVPEALAR"
     gene            complement(1283..>1889)
                     /gene="lpxK"
                     /locus_tag="VN039_07415"
     CDS             complement(1283..>1889)
                     /gene="lpxK"
                     /locus_tag="VN039_07415"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWV45833.1"
                     /translation="FQHRSLHRDVDLVLLDASDAAGLDALLPAGRLREPLTELCRASA
                     IMITRADSRQDVDAIRSRLQAVSCSSEDAVEVVFRPESVMPIVSGERQPVEWCRRKKA
                     WLVSGIGNSRSFHRSAESMGVEILGETAFEDHHDYRHDDIEKIRTNLQATGGEIVLTT
                     EKDGGKLLPLLMPSDSWWMLRLGAEVMRGKERLYRLIDGSR"
BASE COUNT          417 a          589 c          504 g          379 t
ORIGIN      
        1 gcctggcgag cgcctcaggc acccctggaa acagctcaaa ctgatcggag gagttgatgt
       61 agccgggatc agggttgagc gttccatctc gatcgagaaa gatggtgtaa ccggaaagaa
      121 ttttccggtc tgtctggtct attttgtctg tctggtctgt tcggtctatc tggtctgtct
      181 tgtctggacc agatagacca gaaggaccag acagacgaga cagaccggct tcatagactc
      241 tttccaccga aacccttgtc atgcaacggt gatcgatcgg gcattctctg aagaggcagg
      301 gcgcacagtc tacggcttcc cgcacgatcg accgttcttg cccgtaggga gcggtggtgc
      361 gccagtccgt gggaccgaac accgcaacca ccggcacacc gaacgccgcc gcaatatgca
      421 tcggtcctgt atcgttcgta atcagcaagc gacaacgttt cagcaccgcc atgagctccc
      481 gaatggtcgt cgcgccggat aagacgaccg atcgcccgcc gagctgagcc gcaatgtttt
      541 ctcccagcca ttcctctccc ttcgctccca ggatgaccac tgcgacatgc acgctttcag
      601 actgttccac tcgccgcaca agtcgcttcg cgacttcggc aaatcggtcg gacatccaac
      661 gttttgcgct gccgtaggtc gatccgggat tgatgccgat aatgagatcc gacgaatcga
      721 taccggcgga ggcaagccgt acgtcggcca ttcggtcctc gtccgccgaa acgagcagcg
      781 tggggagagt ggggccatca gccagtccca atggtctcaa taaattccaa tagtactcga
      841 cttgatgccc cggcacaggg tccttgggaa cagcgaccgg ctcggtgagc aagaagactc
      901 ttccgtccgt ggcataccca tatctccgtg ggataccggc gagccacgca agaaatgcgg
      961 cttcgaacgc gttctgaaac agcaccgcca aatcgaagcg gtgccgtcgc aacgtaccgg
     1021 cgagcgacca ctttcccgat atccccgcat gagccccctt gtcctcatac accagcatgc
     1081 aatgaagagc cggatgggcg atgaatagct ccgcgatggc gggtttggcg agcatcgtca
     1141 actccgcctc ggggaacaga gaccgcaggc caccgaccgc cggttcgcac atgacggcat
     1201 cgccgatcca attcggcgca cgcaccagaa ttctgcgggg caaattccta cgcacgagct
     1261 ctgaccccat gtctctcacc gattagcgcg acccatcgat caatctgtac agccgctctt
     1321 tcccccgcat gacttccgcc ccaagacgaa gcatccacca ggagtcgctt ggcatgagca
     1381 agggaagcag cttcccaccg tccttttcag tcgtcagaac gatctcgccg ccggtggcct
     1441 gtagattggt gcgaatcttc tcgatatcat cgtggcgata gtcgtgatgg tcttcaaagg
     1501 cggtctcacc caggatctca acccccatgg attcggctga tcgatggaat gatcggctat
     1561 tcccgatgcc gctgaccaac caagcctttt tccttcgaca ccattcaacc ggttgccgct
     1621 ctcccgaaac gatcggcata acggactccg gtcggaaaac aacttcaacc gcgtcttctg
     1681 atgaacatga aacggcttgc aatcggctac gaatcgcgtc cacatcctga cgagaatcgg
     1741 cacgggtgat catgattgca ctcgctcgac acagctcggt caagggttcc cgtagtcttc
     1801 cggcaggaag cagcgcgtca aggcctgcgg catcgctggc atccagcagc acaagatcga
     1861 catcccgatg aagcgatcga tgttgaaat
//
LOCUS       DATMTI010000459         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_484863, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000459 DATMTI010000000
VERSION     DATMTI010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484863"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            574..1212
                     /locus_tag="VN039_07420"
     CDS             574..1212
                     /locus_tag="VN039_07420"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWV45834.1"
                     /translation="MDNDASYGMWWLVLINSIFFIVFALSFTRPRTARDWRSLGVFSA
                     FIVALFAEMYGFHLSIYLISGWLASHYPEVDPFAHDTGHFWHTLLGRQGPAHSDPLHI
                     LSEVLVFIGLILLAVSWRVLYRAQREQTLATSGPYALIRHPQYAAFIIIMLGFLLQWP
                     TLPTLLMFPVLVGMYILLAHEEEAEAHAQFGEVYDGYAAATPAFFPSWRKRV"
     gene            1345..1623
                     /locus_tag="VN039_07425"
     CDS             1345..1623
                     /locus_tag="VN039_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45835.1"
                     /translation="MIFRRPELEKDNPSAPQPYVGPRRRLALVRAAASRAFTEENDFK
                     KTLQDLTLAASRYVDRVPKTRKTGKSLKVLRDAIAEAERILAAEGAKS"
     gene            1654..2529
                     /locus_tag="VN039_07430"
     CDS             1654..2529
                     /locus_tag="VN039_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45836.1"
                     /translation="MTTSYGRFMVCGLGVLAWCWCTAGDTAAHEPSGTSQSDSSDSVQ
                     HRGDIAWDAIEWTERRYKIEALGFKARDETGNSWLGSDEVMIRTDDAKGWTVSGQIGG
                     IDSGDAHHLDSAKSCIVPIRPGIVVLGRTSLCDDSGEAAPLSFGVEFWEKDWFGGFGR
                     DFCSPGSPGPGMHAGPHCANDNAGDDFIGRAHIDLSIQDLEAAMPNVGDEYIETVVLE
                     QCDSGNSCDATYGPDYSFTYRITRMRDTRVGLRPMLDKAMSMVGTYSELDAIAAGLRS
                     LRAPSPRQIEPGMGK"
BASE COUNT          614 a          679 c          805 g          573 t
ORIGIN      
        1 gacagaactc ccggagcctg aaggcctcaa agctcacaca ccgacgtacc accaattgca
       61 ctaacgcgat gagaaaggat tacgcttgag gaggatgaaa gaccgaggtc acgaagactg
      121 aaagatggag tacgggctcg cccttctcgt gaagaccgga gcgacttgcc gattctggtt
      181 cggggcttat cggaggaatc gaaaaatcct cagagactga ttccactggt atatccgaag
      241 tgaggagccc gataagcgtc aggggtgttc ccagcatttg cgacatcacg cagatacaga
      301 gacatacgat caggaatgcc aagcctgttt cacgagacga acagaaaaat gaagtgggac
      361 cgatccgacg cataaaatca ctatactccc ccaagggaag agtgggcaag cgagctcaga
      421 tccccttgta gtccaggcaa cgtcggaagg aaaaccactg ttctctacga gggccgactg
      481 actactccgg ttgtatacgg tagggtgtgg cagttgaagg aaccgccgcc tttctgtttc
      541 ggcatatgtc acacgacggc agagagagga catatggata acgatgcaag ctatgggatg
      601 tggtggctcg tcctcatcaa ttctattttc tttatcgtct tcgcgctgag cttcacgcgt
      661 ccacggacgg cgcgcgattg gcgatctctt ggagtcttct ctgcgtttat cgtggccctg
      721 tttgcggaga tgtatggatt ccacctaagc atctatctga tctcgggatg gttggcgagc
      781 cactatcccg aggtcgatcc gtttgctcac gacacgggcc atttctggca caccttactt
      841 ggcaggcaag gccctgcaca ttctgaccct cttcatattc tgagcgaggt gttggtcttc
      901 atagggctga tcctgctggc ggtgtcctgg cgcgtcttgt atcgggcgca gcgggagcag
      961 accctggcca caagcggacc ctatgccttg atcagacatc cgcagtatgc cgccttcatc
     1021 atcattatgc tgggttttct tctccagtgg ccgacccttc cgaccctgct catgttccct
     1081 gtgctcgtgg ggatgtatat ccttcttgcg cacgaagagg aggcagaggc ccatgcgcaa
     1141 ttcggtgaag tctatgatgg ttatgcggca gcaacgccgg cctttttccc ttcatggaga
     1201 aaacgggtat gagaacccga gagttgaact caggaaagac gagtatatgg ataggatcag
     1261 actcgtgagg cggaatgaca cgatgggtgc tctataaacc gggctcgtgt tatggtacgg
     1321 tgcctacttc ctggaggaaa tacaatgata ttcagacggc cggagctcga gaaagataat
     1381 ccgagcgctc cccaaccgta tgtgggtccg cgtaggaggc tggcgctggt gcgagctgca
     1441 gcatcgaggg cattcacgga agagaatgat ttcaaaaaga cgttgcagga tttgactcta
     1501 gcggcatccc gctatgtgga tcgtgtgccg aaaacaagga aaacgggaaa gagcctgaag
     1561 gtattgcggg atgcgattgc agaagccgag cgaatactcg ctgctgaagg cgctaaatcc
     1621 tgatttgaaa tgacttcaag caggaggaga gcaatgacga cctcgtatgg gcgtttcatg
     1681 gtatgcggct tgggtgtgct ggcatggtgt tggtgcaccg ccggcgatac ggccgcgcat
     1741 gagccctcgg ggacgtctca gtccgacagt tccgattcgg tacagcatcg cggcgatatc
     1801 gcgtgggatg ctatcgagtg gacagagcgg cgctataaga tcgaggcgct gggtttcaag
     1861 gcacgagacg aaaccggcaa cagctggttg ggcagcgacg aggtgatgat tcgcacggat
     1921 gatgccaaag gctggacggt ctccggccag atcggcggca tcgactcggg cgatgctcac
     1981 cacctcgact cggcaaaaag ctgcattgtg ccgatacgtc ccggcatcgt cgtgcttggc
     2041 aggacctccc tctgtgacga tagcggcgaa gccgcaccgc tgagtttcgg ggtcgagttc
     2101 tgggagaagg attggttcgg cggatttggc cgtgattttt gcagcccggg ctccccgggg
     2161 ccgggcatgc atgcagggcc gcactgtgcg aacgacaatg ccggtgacga tttcatcggt
     2221 cgggcccata tcgatctgtc gatacaggac ctggaagccg ctatgcccaa cgtcggcgat
     2281 gaatatatcg aaaccgtcgt actagagcag tgtgacagtg gaaacagttg tgatgcgacc
     2341 tatggaccgg actatagctt tacctatcgc atcacgcgca tgcgcgacac gcgggtcgga
     2401 ttgcgcccga tgctcgataa ggcaatgagc atggtcggta cgtattcaga actcgatgcc
     2461 atcgctgccg gtctacggtc cttgcgcgca ccaagtccgc gccaaatcga gccgggaatg
     2521 ggcaagtagg attgcgagag tggagggcac tctacaggca gactgcggat cttgtcacga
     2581 ggcagcccat cgcaagctca gtgcaagcct gaccatggtg cttcattagc attgacgcaa
     2641 cgaaatatgg atagtctcaa tttgaagtaa g
//
LOCUS       DATMTI010000460         1691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_654321, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000460 DATMTI010000000
VERSION     DATMTI010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654321"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VN039_07435"
     CDS             <1..586
                     /locus_tag="VN039_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45837.1"
                     /translation="GIAKPEQHWSFNWVECCDGPCDVQCGNRHPCPEYLRSIPGAWDQ
                     YVKDWTAAKQDELQRMGKAPAPAPGIDWSKFHKPVPIPALSKTDVNKNDTAPGIQSSE
                     GTDFVFISDVLEPLEDVDRLAVGDRSSAKTGPKIAKGERFLAAWLFKAISGPDKDCYF
                     ALTPAPFWTRVNYRDAEGKMYVKRVADAPLKGTP"
     gene            586..810
                     /locus_tag="VN039_07440"
     CDS             586..810
                     /locus_tag="VN039_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45838.1"
                     /translation="MADERYVHRTSRESVMARQVTHHAEIVRNYWGEEESAEIGDYVI
                     YHPFHERVDEETFHEEYIPAQNYPATQSGR"
     gene            758..1042
                     /locus_tag="VN039_07445"
     CDS             758..1042
                     /locus_tag="VN039_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45839.1"
                     /translation="MKSTFLRRITPQLKAAAKRIAIATALAVVPLIIQEVNGAKVDPQ
                     YAIYILIASRLLEGVYDSLRDKYGDVHASDVTPNTPVVPAAPQTRPKIIK"
     gene            1052..1342
                     /locus_tag="VN039_07450"
     CDS             1052..1342
                     /locus_tag="VN039_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45840.1"
                     /translation="MDHYTPEARAKRLKYIDITFQHGPIQDVGVNGAHIEDVLDILIS
                     RLRAFNQEDSPTRCRENSLAITHLEEAKNWLVSRTLKRVEQGVEGYQIPHRE"
     gene            complement(1384..>1691)
                     /locus_tag="VN039_07455"
     CDS             complement(1384..>1691)
                     /locus_tag="VN039_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45841.1"
                     /translation="VKIVVDQAEIDYFFTGSPRQTQKVKVPVMDPSFTEAYLRDRLRP
                     FLNYETAGVLPPILPSTYVQKRNGTTEIKRHWMCNRCPVRALCDALDEAERPEDDEE"
BASE COUNT          368 a          518 c          498 g          307 t
ORIGIN      
        1 ggggatcgcc aaaccggaac agcactggtc attcaactgg gtggagtgct gcgatggtcc
       61 ctgcgacgtg caatgtggca atcgccaccc ctgcccggaa tatctccgca gcatcccggg
      121 cgcgtgggac cagtatgtga aggactggac tgcggccaaa caggatgaat tgcaacgcat
      181 gggaaaggca ccggcaccag ccccgggtat cgactggtcg aagttccaca agccggttcc
      241 gatcccggcg ctctccaaga cggacgtgaa caagaacgac accgccccag gcatccagtc
      301 cagtgagggg acggacttcg tgttcatctc cgacgttctg gagccgctgg aggatgtgga
      361 ccgactggcg gtgggtgatc gctccagcgc gaaaacggga cccaaaatcg ccaagggtga
      421 gcgcttcctt gccgcgtggc tcttcaaggc gatcagtggc ccggacaagg attgctactt
      481 tgccctgacc ccggcgccct tctggacccg cgtcaactac cgggacgcgg agggcaagat
      541 gtacgtcaag cgggtcgcgg atgcgccact caaaggaacc ccgtaatggc cgatgagcgc
      601 tatgtgcatc gcaccagccg ggagtccgtc atggcccggc aggtcacgca ccacgcggag
      661 atcgtgcgca actactgggg ggaagaggag tcggcggaga tcggtgacta cgtgatttac
      721 cacccgttcc atgagcgggt tgatgaggaa accttccatg aagagtacat tcctgcgcag
      781 aattaccccg caactcaaag cggccgctaa gcgcatcgcg attgcgaccg cgcttgcggt
      841 ggtgcccctg atcattcagg aggtcaatgg cgccaaggtg gacccgcagt atgccatcta
      901 catcctgatc gcctcccggc tcttggaggg cgtctacgac tccctacggg acaagtatgg
      961 ggacgtccac gccagcgacg tgacccccaa caccccggtg gttccggccg caccgcagac
     1021 ccggccgaag attatcaagt gaggaccact cttggaccac tacaccccag aagcgcgagc
     1081 caagcggttg aagtacatcg acattacctt ccaacatggc cccatccaag atgttggcgt
     1141 caatggcgcc cacatcgaag atgtgcttga catactgatc tcccgactcc gggcattcaa
     1201 ccaggaggac agtcccaccc ggtgccggga gaacagcctt gcgatcaccc atctggaaga
     1261 ggcaaaaaac tggttggtca gtcgcaccct gaaacgggtt gaacagggcg tggaggggta
     1321 ccaaatccct cacagggagt aaccatggta gccgacattg ttctggcatt tgtcctgatt
     1381 atcctattcc tcatcatcct ccgggcgttc ggcctcatct agggcgtcac agagtgctct
     1441 caccggacac cggttgcaca tccaatgacg cttgatctcg gtggttccgt tgcgcttctg
     1501 gacatacgta gacggcagga tcggtggcaa cacccctgcc gtctcgtagt tcaggaacgg
     1561 tcgcagacga tcccgaagat acgcctcggt gaaggagggg tccatcaccg gcaccttgac
     1621 cttctgagtc tggcgcggtg acccggtgaa gaagtagtcg atctcagcct gatccaccac
     1681 gatcttcacc c
//
LOCUS       DATMTI010000461         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_188159, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000461 DATMTI010000000
VERSION     DATMTI010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188159"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..522
                     /locus_tag="VN039_07460"
     CDS             64..522
                     /locus_tag="VN039_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45842.1"
                     /translation="MSTMSFCLVLLANALALISLGGGFYEFLVVDPFWPKRPELIQPS
                     RGGISRRRFWIPAHTLFELALLSALVMVWSQSVLRQWLLIALASHAIMRLWSAFDFIP
                     HALAFERADPASVTETAARRWTSRSLGRFPLDLVTCGAMMAAFATAVELR"
     gene            543..1088
                     /locus_tag="VN039_07465"
     CDS             543..1088
                     /locus_tag="VN039_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWV45843.1"
                     /translation="MKRTPTPGRAGTPTSKAQMVARVGIQVRQMGAQSVLTSQVIAGR
                     FDLHTTDLECLDLIYLRQNPSAGELAKATGLTSGAVTALIDRLEEAGFVKRVADPTDR
                     RRLHVQIRPTAIAPIKAVYEPMQARMFDLWSSYSIQELAVILDFLSRSTNLAVECTEA
                     IRHKGSSKLSRSRAKRMNRKE"
     gene            1261..1683
                     /locus_tag="VN039_07470"
     CDS             1261..1683
                     /locus_tag="VN039_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV45844.1"
                     /translation="MKPRITLITTGVDDLEQSLRFYRNGLGLKTEGIVGTQFEHGAVV
                     FFELQVGLRLALWPRTSIAHDSGLPVGRPSHTEFTIAHNVASKAEVDAVMEQARCAGA
                     VIVKPARDAFWGGYTGYFQDPDQHLWEIAWNPEWVAQE"
     gene            2008..2607
                     /locus_tag="VN039_07475"
     CDS             2008..2607
                     /locus_tag="VN039_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HWV45845.1"
                     /translation="MKWNPCTTGLDLELERLRLEWAIRWHPVPKVLDWGADDEAQWLV
                     TAALPGEGAVMEPWRMRPLDAVRAIARGLRMLHDTLPVADCPFDWSVEARRSRCIHGE
                     RLLVPSIDRLVVCHGDACSPNTIIASDGSTAGHVDLGSLGVADRWADLAVASRSLEYN
                     FGPGWEGEFFKVYGIERDEERISFYRLLWDSEGMIGLAS"
BASE COUNT          577 a          728 c          811 g          654 t
ORIGIN      
        1 atatttaata attaaactat accaacgttg gtccgtccta taatgaattg acgcggaggt
       61 tatatgtcga cgatgtcatt ttgcctcgtc cttcttgcca atgccctggc cttgatcagc
      121 ctgggcggag gtttctacga gtttctggtg gtggacccct tttggccgaa acggccggaa
      181 ctcattcaac cctctcgcgg gggaatctcg cgccggcggt tttggattcc tgctcatacc
      241 ctgtttgagc tcgcgctgct tagcgcactc gtcatggtct ggtcgcagtc agttcttcga
      301 cagtggctgt tgatcgctct agcgagccat gcgattatgc gtctctggtc ggcgttcgat
      361 tttattcctc atgcgctggc cttcgaacga gcggatcctg cttcggtcac agaaaccgca
      421 gcgcgccggt ggacgtcccg cagtctgggc cggtttccgc tcgatctcgt cacatgtggg
      481 gcaatgatgg ccgcttttgc cacggcagtt gagcttcgct aacgcaggtc cagggaaatc
      541 ttatgaagcg gactccgaca cccggtagag ccggtacgcc gacatccaaa gcccaaatgg
      601 tggcccgtgt ggggattcaa gtgcggcaaa tgggcgcgca aagcgtcttg accagccagg
      661 tcattgccgg ccggttcgat ctgcacacca cagatctgga gtgtctcgat ctcatttatt
      721 tgcgtcagaa tccttcggca ggagagttag ccaaggccac gggcctgaca tcgggcgcag
      781 tgaccgcatt gatcgaccgt cttgaggaag ccggattcgt caaacgtgtg gcggatccta
      841 cggatcgccg acgtctgcac gtgcagattc gcccgaccgc tattgcgccg attaaagcgg
      901 tatatgagcc gatgcaggcc cgcatgttcg atctctggtc ctcttattcg attcaagagt
      961 tggcagtcat tcttgatttc ctatcccgta gtaccaacct cgcagtcgaa tgcactgaag
     1021 cgattcgaca taaagggtct tcaaagctct cacgaagccg cgccaaaaga atgaatcgta
     1081 aggagtgaag tctcagcgct ttatggggtt ggttgggtca gttagatgct aatgagaaag
     1141 tagcgtttgg tcggcgggac taacgtgcag gggcaagcca tgctatcgct gccgcgcatc
     1201 tcgtgactga aaatggtggc ctgcatatct gtttcagctt gccgccgaaa ggatcttcac
     1261 atgaagccgc gtattacgct catcacgacc ggtgttgatg atttggaaca atctctccgg
     1321 ttctatcgca acggactagg gcttaagacc gaaggcatcg tcggtaccca gtttgaacat
     1381 ggagcggtgg tgttctttga gttacaagtc ggcttacggc ttgcgctctg gcctcgcaca
     1441 agcatcgctc acgattctgg tctacctgtg ggtcgcccga gccacacgga attcactatc
     1501 gcgcacaacg tggcctcaaa ggccgaggtc gatgccgtca tggagcaagc caggtgtgca
     1561 ggcgcagtga tcgtcaagcc ggctcgtgat gcattttggg gtgggtacac aggatatttc
     1621 caagacccag accagcatct gtgggaaatt gcatggaatc cggaatgggt tgcacaagag
     1681 tgagatccag cgatcccatg ccggttactc gtgtacttgt ccagtatgcg cccaagtaaa
     1741 gttcctaggt ttcagaatcg attgagaaca tcgttttgaa gagggcttgc ctctttaata
     1801 tgccgatctc acaggctgat tgacgtgtac cagcgccgtc agtacccagc aaaggtacgc
     1861 tagcatgagc tgttactgct gatgccaacg accatgacaa ctctatctat tcctttggtt
     1921 gtccgccgac tctccggcaa ccacgaacca cagttggtat ggacaaacga acgcggtggc
     1981 ctaacctttc aggccgggaa tcgtttcgtg aaatggaatc cgtgcaccac aggcctcgat
     2041 cttgaactag agcggctcag gctggagtgg gcgatccgtt ggcatccggt accgaaggtg
     2101 ctcgactggg gagccgatga tgaggcgcaa tggctggtta cggccgcgct gccaggtgaa
     2161 ggagcggtca tggaaccatg gcgcatgcga ccgctcgatg cggttcgcgc gattgcgcgg
     2221 gggcttcgca tgttgcatga cacgctccct gttgccgatt gccctttcga ttggtcggtt
     2281 gaagctcgaa gaagccgctg catccacggc gaacgtctgc ttgtgccgtc cattgaccgc
     2341 ctcgtggttt gtcatggcga cgcctgttca ccaaatacga tcattgcatc ggatggtagc
     2401 acggccggcc acgtcgatct cggctcgctt ggtgtcgcgg accgttgggc cgacttggct
     2461 gttgcaagcc ggagcctcga gtacaacttc ggaccaggtt gggaaggtga gttcttcaag
     2521 gtgtacggca tcgagcgcga cgaggagcgg atctctttct accgcctcct ctgggacagc
     2581 gagggtatga ttggtcttgc atcataatat gaacggagac ggaagagctc aaagggtagt
     2641 tggacttctg gacaccaggc tggtgaactt cgttagtgag ccaaagggat gcgtatgcag
     2701 agcgatgaag aggaaatccg gcaactcgtt tccacctgga tggcggcaag caaagccggc
     2761 gatgttgaaa
//
LOCUS       DATMTI010000462         3271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_484932, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000462 DATMTI010000000
VERSION     DATMTI010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484932"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VN039_07480"
     CDS             <1..1294
                     /locus_tag="VN039_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV45846.1"
                     /translation="ITVTLKPEAQWRSDMTWDKLLDEMDAKLRIPGFPNIWWMPIQTR
                     TEMITTGVRSPVGIKVLGPDLKTIKRIGVEIEQALATVPGTKSAFAERLNEGYYLDVI
                     VNRREAARYSLTVGDVQAVITTAIGGETVTTMVEGRERYPVNVRYKRELRDDPDRLKR
                     VLIPTPTGAQIPLGQIAEMVITQGPPSIADEAGALAGLVSVSVGGRDLRGYVQDAQRA
                     VRDRVTVPSGYRLIWTGQYEHLVRAEERLKLVIPVTLAVILLLLYLNFRSLAKSLIVL
                     LSVPFAVIGAIWYLHYLGYNLSVAVWVGIIALAGVAAETGVVMLVYLDEAYERRVREG
                     RMATAQDLRESIMEGAVQRVRPKMMTVAAIMGGLLPIMWTTGTGADVMKRISAPMIGG
                     MVSSTVLTLIVIPVLYSMWRRVQLRSAMSSPASKDLFA"
     gene            complement(1395..1955)
                     /locus_tag="VN039_07485"
     CDS             complement(1395..1955)
                     /locus_tag="VN039_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWV45847.1"
                     /translation="MRNILTVGLITVLGTGILWAGGPADRQERTHGYGLGRLATDQEI
                     RVWNIDVAPTGEGLPAGQGTAKQGAAIFAARCATCHGPTGQEGPMDRLVGGAGTLASQ
                     HPIKTIGSYWPFATTLYDYVHRAMPFPAPQSLSPDEVYSVVAWLLYRNGIIAEDVVLD
                     ARSLPGISMPNRQGFLPDPQPDVPKP"
     gene            complement(1948..3222)
                     /gene="soxC"
                     /locus_tag="VN039_07490"
     CDS             complement(1948..3222)
                     /gene="soxC"
                     /locus_tag="VN039_07490"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HWV45848.1"
                     /translation="MMNRFQEEGPSGGDVRPDAQDESLLNRRSLLAGTAGLVGAMLME
                     SMSPSAEATTEGSPEDPTRVPGAVPTAYGQRSAFEKSVRIPSSWSSSLTPLQDSHGIL
                     TPSALHFERHHNGVPTIHPAHHRLLIHGMVDQPWTFTMDDLKRFPSVSRLAFIECSGN
                     SGSEWRGPTGQTVQHTHGLTSTSEWTGVALKTVLQEVGVNPDASWMLAEGSDAAAMTR
                     SLPLASILEEAILCYAQNGEPLRPEQGYPLRLLIPGWEGNTCIKWLRRLKLGKAPFMT
                     REETSHYTDLMPDGTARQFTMVMEAKSVITSPSGGQRIQPGFLEIRGLAWSGRGRIAM
                     VEVSTDGGRSWRQAALQEPILPKCHTRFRLPWRWNGEDVILQSRCLDDTGYRQPDHVA
                     LVAIRGVNSVYHYNAIQSWHIAADGKVSNVHA"
BASE COUNT          621 a          947 c         1041 g          662 t
ORIGIN      
        1 gatcactgtc accttgaaac cagaagcgca atggcggtca gacatgactt gggacaagtt
       61 gttggatgag atggatgcga agctgcgcat cccaggattt ccaaacatct ggtggatgcc
      121 gatccagaca cgcacggaga tgatcacgac cggtgtccgg agcccggtcg ggatcaaggt
      181 gctggggccg gatttaaaga cgatcaagcg aatcggcgta gaaatcgaac aggccttggc
      241 gaccgtgccc ggcacgaaaa gcgccttcgc cgaacggctc aatgagggct attacttgga
      301 tgtaatcgtg aaccggcgtg aggcggcccg ttatagcttg acggtgggag acgtgcaagc
      361 ggtaattacc actgccatcg gaggggaaac ggtgaccacc atggtggagg gacgggaacg
      421 atatccggtg aacgttcgct acaagcgcga gttacgcgac gatccggacc ggctcaaacg
      481 agtgctgatc cccacaccga ccggcgcgca gatccctctt ggacaaatcg cagagatggt
      541 gatcacgcag gggccgccgt cgatcgcgga tgaagcaggg gcgctggccg gtctggtgtc
      601 ggtgtcggtc ggtggccgcg acttgcgtgg ctatgtccag gatgcgcaac gtgcggtgcg
      661 agaccgagtg acagtaccgt ccggctaccg gctgatttgg accggtcaat acgagcatct
      721 ggtgcgggcg gaagagcgat taaaactggt gatcccggtg actctcgccg tgattctatt
      781 gctcctgtat ctcaatttcc gatcgctcgc gaaatcgctg atcgttcttc tctctgtccc
      841 ctttgccgtg atcggagcca tctggtacct ccattaccta ggctacaacc tcagcgtagc
      901 tgtgtgggtg ggaatcattg cattggccgg cgtggcggcg gagacaggcg tggtcatgct
      961 cgtctatctc gacgaagcgt atgagcggcg tgtgcgcgaa ggtcgcatgg ccacggcgca
     1021 ggacctccgc gagtccatca tggagggtgc ggttcaacga gtgagaccga agatgatgac
     1081 ggtggcggcg atcatgggcg gcttgttgcc gatcatgtgg acgactggca ccggagccga
     1141 cgtgatgaag cggatttccg cccctatgat cggcggcatg gtcagctcga cagtgctcac
     1201 gttgatcgtc attcccgtcc tttattccat gtggcgacgt gtgcagctcc gttctgcgat
     1261 gtcgagtcct gcctcgaaag atcttttcgc ttgagggcct tcatccggat gaagcgcata
     1321 gcgagattcc cgaagaacgg tgctagacct ggagcgaaca atcgtgagcc gcggcgcggc
     1381 gtctgaccca ggcgtcaggg tttaggcacg tccggttgag gatcaggcag gaaaccctgg
     1441 cggttcggca tggagatgcc gggcaacgat ctggcatcga gcacgacatc ctcggcaata
     1501 atgccgttcc ggtaaagcaa ccaggccacg accgagtaga cttcgtccgg cgagaggctt
     1561 tgcggagcgg gaaacggcat ggcgcgatgc acgtaatcgt acagcgtcgt cgcgaagggc
     1621 cagtaactgc cgattgtttt gatcgggtgt tgactcgcca atgtgccggc gccgccgact
     1681 aagcgatcca tcgggccttc ctgcccagtc ggtccatgac aagtcgcgca acgtgcagca
     1741 aagatggccg ccccttgttt ggcggtccct tggccggcag gcaatccctc gccggtcggc
     1801 gccacatcga tgttccatac ccgaatttcc tggtctgtgg cgagccgtcc caatccatag
     1861 ccatgggtcc gttcctgtcg gtccgccggg ccgccggccc ataagatccc tgtgccaagg
     1921 acggtgatga gccccaccgt cagaatgtta cgcatggaca ttgctcacct tcccgtctgc
     1981 ggcaatgtgc cagctttgga tcgcattgta gtggtagacc gagttcactc cacgtatcgc
     2041 caccaaggcc acgtggtccg gttgccggta tcctgtgtcg tccaggcagc ggctctgcag
     2101 aatcacgtcc tctccgttcc accgccaagg caagcgaaac ctggtatggc acttgggcag
     2161 aatcggctcc tgtaaggccg cctgccgcca ggatcgcccc ccgtccgtgc tcacctcaac
     2221 catcgcgatg cggcccctgc cgctccatgc caacccacgg atctccagga aaccaggctg
     2281 gatacgttgt ccacccgagg gagatgtaat gacggatttg gcttccatga ccatggtgaa
     2341 ttggcgggcc gtgccgtccg gcatcagatc cgtatagtgg gatgtctctt cgcgggtcat
     2401 gaagggcgcc ttgccgagct tcagccgccg cagccatttg atgcaggtgt tgccttccca
     2461 gccggggatg agcaggcgga gcggatagcc ctgttctggg cggagcggct cgccgttttg
     2521 ggcgtagcag aggatcgcct cctccagaat ggacgccagt ggaaggctgc gcgtcatagc
     2581 cgccgcatcg ctgccttcgg ccaacatcca ggatgcgtcg ggattcactc cgacttcttg
     2641 caggacggtc ttcaaggcca cgccggtcca ttcactcgtg ctcgtcaggc cgtgggtatg
     2701 ctgcacggtc tgcccggtcg gtccgcgcca ttcgctcccc gaattgcccg aacattcgat
     2761 gaaggccagc cgtgaaacgg aaggaaatcg ctttaaatcg tccatggtga aggtccatgg
     2821 ctggtccacc atgccgtgga tgaggagccg gtggtgtgcc gggtggatgg tcggcacgcc
     2881 gttgtggtgc cgttcgaagt gcaaggcgga aggagtcagg atcccatgcg agtcttgtag
     2941 gggtgtcagc gacgacgacc aggaacttgg gatacggacg gatttttcga aggcggatcg
     3001 ctgtccatag gcagtcggca ccgcaccggg cacccgcgtg ggatcttccg gcgaaccctc
     3061 cgtggtcgct tcggcggatg gactcatcga ttccatgagc atcgcgccca ctaagccagc
     3121 cgttccggcc aggaggctcc ggcgattcag aagggattca tcttgagcat cgggcctgac
     3181 gtcgcctcct gatggtccct cttcctgaaa gcggttcatc atggaatgct cctctgttac
     3241 tgggcggcgc agaggtcaca tgcggatgcc c
//
LOCUS       DATMTI010000463         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_781631, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000463 DATMTI010000000
VERSION     DATMTI010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781631"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VN039_07495"
     CDS             complement(<1..463)
                     /locus_tag="VN039_07495"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV45849.1"
                     /translation="MNMQPFELIGADGKRLIGDCAAGADRQILFITGFLSKRWGNKSK
                     ALAQWCQEKGWGFCCYDVRGFGDSEGQFTDYTLSDWIADAQAVLESLESGAPITIVGN
                     SLGSWIAWLVAQEFPIVEELVLIAPAFNMMGERAKTIHKERLHDWHTAGWMP"
     gene            complement(460..1182)
                     /locus_tag="VN039_07500"
     CDS             complement(460..1182)
                     /locus_tag="VN039_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45850.1"
                     /translation="MREVEVIVGGLKIRLAGGSDGHGGGDGPVVMLLHGFGASGDDLV
                     PLADVIQVPTGTRWIFPEAPLALNMGFGDSRAWWIIDFARIQEDRAAGRIRDLSVEIP
                     QGLALAREGFLAFLKELHRQLPVDYKRTIIGGFSQGAMLTCDAVLHTAYPFAGLVQLS
                     GNLLAQAVWDPLMSKRKGLPVFQSHGTSDDILPHIGAERLRDALIQSDLVVEWRSFRG
                     GHEIPEAVLQRLSGFITKAFKI"
     gene            complement(1277..2752)
                     /locus_tag="VN039_07505"
     CDS             complement(1277..2752)
                     /locus_tag="VN039_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HWV45851.1"
                     /translation="MTRLTLETLRFDNTYARLPQAFYARLNPTPFNAPPYLVHANRGA
                     AELIDLDPAQLTRPEFAALFGGSVLAPGMEPLAMLYSGHQFGVYVPQLGDGRAILLGE
                     AKNDQGERWDLHLKGAGMTPFSRDGDGRAVLRSTIREYLCCAAMQGLSIPTTQALCLV
                     GSDDKVYREQVETGAMLVRMAPSHVRFGTFEVFYYRKQHEFLKVLADYVIDQHFPHLR
                     EAGDTYPRFFTEVVERTAKLIAQWQAVGWAHGVMNTDNMSILGLTLDYGPYGFMDDYD
                     AGFICNHSDHNGRYAFNQQPYIGLWNLSCLAQALLPLAEKEALKAGLETYQLLFEREY
                     LTLMRTKFGLVVERAEDDELIRDFLGLLQGSHADYTIVLRELSRFSTADGATNETLRE
                     HFLNRDRFDEWAARYRDRLRNERSCDDERCDRMNRVNPKYVLRNYLAQTAIEKAHNKD
                     FSEIDRLFTLLQDPFTEQPGMDAYALPPPNWGKHLAVSCSS"
     gene            complement(2749..3111)
                     /locus_tag="VN039_07510"
     CDS             complement(2749..3111)
                     /locus_tag="VN039_07510"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HWV45852.1"
                     /translation="MENSIRNAIIKFEQEFLGRGPDEVRALVARDLVVVRLKGVLTPA
                     ERQLAKTAEGVEMVKRLRQNLIAQGRDKLCEQVSEITGAKILGLFTDIDVQLGERVFV
                     FTMDRDIQDRQPVKYNLP"
     gene            3492..>4376
                     /locus_tag="VN039_07515"
     CDS             3492..>4376
                     /locus_tag="VN039_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWV45853.1"
                     /translation="MMSAVLLVPLLPLIAALIVGVGRETSRHARAKIAAWPIGAAFCG
                     AIATLYVVATEGPITLRLYDPVASTVLAVPIGLYIDRLSAVMMVLISGIGTIIYTYSV
                     EYMYQDLHERRYLSLIGLTVFVLLCMVSSANLLMLFVLWQLLSYLLYLLVHNHSHQET
                     LKSAFRTFMLLRVGDVAFLGGTVLAYSLYGTLEFPDLFAAAAQSTATVSLIPGVEFNG
                     ATAVTLLLLVGGMSKSAQFPFYGWLPRYLYAPTSVTALLHAGIINAAGFLINRLAPLF
                     GMSSTTLHIALVIGTLTAI"
BASE COUNT          907 a         1285 c         1189 g          995 t
ORIGIN      
        1 atggcatcca tccggcggta tgccaatcgt ggagccgttc cttgtgaata gtttttgctc
       61 gttctcccat catattaaac gccggtgcga tcaggacgag ttcttccacg atggggaact
      121 cctgagctac cagccaagca atccaacttc cgagtgaatt gccgactatg gtaataggtg
      181 ctccagattc aagggattcc aagactgctt gtgcatcggc gatccaatcc gagagggtgt
      241 agtctgtgaa ctgtccctca gagtcaccga acccgcgcac gtcgtaacaa cagaaccccc
      301 aacccttctc ctgacaccat tgcgcgagcg ccttgctctt attgccccag cgcttggaaa
      361 gaaagccggt gatgaagagg atctgccgat cggctccagc ggcgcaatcc ccgatgagac
      421 gttttccatc ggcgccgatg agttcgaatg gctgcatgtt catattttga atgccttcgt
      481 gatgaacccg cttagtcgct gtagcacggc ttccggaatt tcatgcccgc ctcggaagct
      541 ccgccactcg acgacgagat ccgattgaat cagtgcgtct cgcagacgtt ccgcaccgat
      601 atgtggaaga atgtcgtccg acgtgccgtg gctttgaaag accggcagtc ctttgcgttt
      661 cgacatgagc gggtcccaca ccgcctgagc caggagattc ccggagagct gcacgagacc
      721 ggcaaatgga taggctgtat gcaagacggc atcgcaggtg agcatcgccc cttgtgaaaa
      781 tccaccgatg atcgttctct tgtagtcgac cgggagctgt cggtgtaatt ccttgagaaa
      841 tgccagaaat ccttctcgag ccaaggccag gccctgcgga atctccaccg acagatcacg
      901 aatgcgaccg gcggcgcgat cctcctggat acgcgcgaag tcaatgatcc accatgcgcg
      961 agaatcgccg aagcccatat tgagcgcgag cggagcttct ggaaagatcc agcgcgtccc
     1021 ggtgggaact tggatcacat ctgcgagcgg gaccagatcg tcacccgagg cgccgaagcc
     1081 atggagcagc atgacgaccg ggccgtcccc gccgccatga ccgtctgagc cgccggcgag
     1141 tcgaatcttt aatccgccca ctataacttc aacttcccgc atgtcactcc ctagctacga
     1201 agagatgctg tcaaaaggac ccgcgcgcga cactctacaa tagaaggtcc agagagtgaa
     1261 ggaggtcgag gttaacttac gatgaacaac tcacggccaa atgcttgccc cagttcggtg
     1321 gaggaagcgc ataggcgtcc atgccgggct gctcggtaaa ggggtcttgg agcaaagtga
     1381 agagtcggtc gatttcggag aagtctttgt tatgggcctt ctcaatcgcc gtctgagcga
     1441 gatagttacg cagtacgtac ttgggattca cacgattcat acggtcacat cgctcatcgt
     1501 cgcagctgcg ttcattccgc agtcgatccc gatagcgcgc cgcccattcg tcgaaacggt
     1561 cacggttgag gaagtgttcg cgcaacgttt cgttggttgc gccgtcagcc gtggagaacc
     1621 tactcaactc tctcaagaca atcgtataat ccgcatggct gccctggagg agacctaaaa
     1681 agtcgcgaat gagttcgtcg tcttctgctc gctcaacgac caatccgaat ttcgtccgca
     1741 tgagcgtcaa atactcccgc tcgaatagga gttgataggt ctcaaggccg gccttcaacg
     1801 cctccttttc cgccaacggc aacaaggctt gtgccaagca gctcagattc caaagaccga
     1861 tgtagggctg ctggttgaaa gcatagcggc cgttgtgatc cgagtgattg cagatgaatc
     1921 ccgcatcgta atcgtccatg aagccatacg gaccatagtc cagcgtcagt cccagaatcg
     1981 acatgttatc cgtgttcatc accccgtgcg cccagccgac ggcctgccat tgggcgatca
     2041 gcttcgccgt gcgctcaacg acctctgtga agaagcgcgg gtatgtgtct ccggcctcac
     2101 gaagatgcgg gaagtgttga tcaatcacgt agtccgcgag aaccttcaag aattcatgct
     2161 gtttccggta atagaagacc tcgaacgtgc cgaaacggac atgtgacggc gccatacgga
     2221 ccaacatcgc gccggtttcg acctgttcgc gatagacctt gtcgtcgctg ccgacgaggc
     2281 agagcgcctg tgtagttgga atactgagac cctgcatggc ggcgcagcaa agatactcac
     2341 ggatcgtcga gcgcagcacc gcccggccgt ctccatcacg cgaaaatggg gtcattcctg
     2401 cgcccttgag atgcagatcc caccgttccc cttggtcgtt cttcgcttcg ccgagaagaa
     2461 tggcccgtcc atcgccgagt tgcggcacgt agacaccgaa ttgatggccg gagtacagca
     2521 tggcgagagg ctccatcccc ggcgccaata cactcccgcc gaacagtgcc gcgaactccg
     2581 gccgcgtaag ttgcgccggg tcaagatcga tcagctcagc cgctcctcga ttggcatgca
     2641 ccagataggg aggagcgtta aacggagtcg gattcaaccg cgcgtaaaag gcttgaggaa
     2701 gacgggcgta ggtattgtcg aagcgtagcg tttccagtgt gagccgtgtc atggaaggtt
     2761 atattttacc ggctgccggt cctggatatc gcgatccatt gtaaagacaa agactcgctc
     2821 gccgagctgc acatcaatat ccgtgaagag tcccaagatt ttggcaccgg tgatctcgct
     2881 gacttgttcg cagagcttgt cgcggccctg cgcgattaaa ttttgccgga gccgcttcac
     2941 catctccaca ccttctgcgg tcttcgccaa ctgccgctcg gccggcgtca gaacgccttt
     3001 gagccggacg accacgagat cccgggccac taatgcacgg acttcatcag ggcctcgtcc
     3061 cagaaattcc tgttcgaact tgatgatcgc gttgcgtatc gaattttcca tagccgagct
     3121 tcacatatca tcctctagga ttgcatgaca agaagatgcg cgtcgcactg ggccttttgt
     3181 cttaatcaat cactgatcat gggcctttcg ggccaagaat cttcggttga gagacccggt
     3241 cgttcgattc gttcttgacg ctgctcgctc atccatatta tgcatggacc gttttcacca
     3301 aacggcgatg ctgccggtcc tggtagtgta acaccgtcac ggatcggtgg gatcccgagt
     3361 tcccgcgcga taggtcgtag ggtgaccggc agttcatgcg agagaggccg gtcgcagtac
     3421 aagccaaccg agctatatcg acaggtgtcg agcataccgg ttggcttttg tgttttcgag
     3481 actttgaggt gatgatgtcc gcggtcttgc ttgtgccgct gcttccattg atcgccgctc
     3541 ttatcgtggg cgtcggtcgt gagacttcgc gccacgctcg cgcgaagatc gccgcctggc
     3601 cgatcggtgc ggccttctgc ggcgcaatcg ccaccctcta cgtcgtcgct acggaaggcc
     3661 cgattacgct tcgtctgtac gatccggtcg cgtccactgt gttggccgtt ccgatcggcc
     3721 tgtacatcga tcggctcagc gcggtcatga tggtcttgat ctccggcatc ggtacgatta
     3781 tttacaccta ctccgtcgag tacatgtacc aggatttgca cgagcgccgg tatctctcct
     3841 tgatcggact gaccgtgttt gtccttctct gcatggtgtc cagcgcaaac ttgctgatgc
     3901 tcttcgtgtt gtggcaattg ctgagttacc ttctctacct cctggtccac aatcatagcc
     3961 accaagagac actgaaaagc gcatttcgca ccttcatgct tttgcgggtc ggcgatgtcg
     4021 cctttttagg tggaaccgtg ctggcttatt cactgtacgg gacattggaa tttcctgatc
     4081 tgtttgccgc cgctgcgcaa tcgacggcga ccgtgtccct gatccccggc gttgaattca
     4141 acggcgcgac agcggtcacc ttgctgctcc ttgtcggcgg aatgagtaaa tccgcccaat
     4201 ttccatttta cggctggctt ccccggtatc tctatgcccc gacgtccgtg actgccttac
     4261 tgcacgcagg cattatcaat gccgccggct ttctcattaa ccgcctggca ccgctcttcg
     4321 ggatgagctc gaccactttg cacatcgccc tcgtgatcgg aacgctgacg gccatc
//
LOCUS       DATMTI010000464         1595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_484957, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000464 DATMTI010000000
VERSION     DATMTI010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484957"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VN039_07520"
     CDS             <1..1310
                     /locus_tag="VN039_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWV45854.1"
                     /translation="QNGKVPSGCCIAFVCLLALVGLNGQHDDRAIAQELNGQASSDWV
                     AEIEKVFIRSEDCKQCHERHYEEWKGAREQTTDLKTFGRVDAALLHGTSLESPVFRTV
                     LGLWKQTNPTPDEQRRCLLCHVPSASAFPQHAEKIAAQVMAGTPKIEGIGCAACHLMN
                     GLEKRLHSPPTFTLQPGGTFYGPYSNPEENLVHPSALSDHFRAASFCASCHFDKVKDV
                     AQKELPGEILQGTICQDCHMESSTGSSTSRRGAMTRAIGRHWFRGVVASGTMLKNRNL
                     QAEWMPRIDIEVAKIGPVVEGTGVVKVGSLPHSFPDGDPVLKQFFLTVRVKDGAGNTL
                     TEETKQFGLPFDKILRGPIPDPFIKGGNTRKVPFSLTLPAGTAAASIEAVLTYSLLPT
                     PEPFLQERYLATLESETGREEARRIIQEYTQRRFLTYRVKSLS"
BASE COUNT          346 a          426 c          462 g          361 t
ORIGIN      
        1 gacagaacgg taaggtgcca tcaggctgct gcatagcatt cgtctgtctc ttggcactcg
       61 tcggtctgaa cggtcagcat gacgaccggg caattgctca agagttgaat ggtcaggctt
      121 cgtctgactg ggtggcggaa atcgaaaaag tcttcatccg ttctgaagac tgtaagcagt
      181 gtcatgagcg tcattacgaa gagtggaagg gcgccagaga gcagacgacg gacttgaaga
      241 ccttcggtcg tgtggatgcc gctcttcttc acggcacgtc gttggagtca cccgtctttc
      301 gaaccgtgtt gggtctttgg aaacagacga atccgacgcc cgatgagcag cgccgctgtc
      361 tattgtgcca tgtgccttcc gccagcgctt ttccccagca tgccgagaaa attgcggcgc
      421 aagtaatggc cggcacgcca aaaattgaag ggatcggctg tgccgcctgc catttgatga
      481 atgggctgga aaagaggttg cactcaccgc cgacattcac attgcagccc ggcggcacgt
      541 tctatggtcc ttattccaat cctgaggaga acctcgtcca tccctccgcg ctgtcggatc
      601 acttccgagc ggccagtttt tgtgcctctt gtcatttcga taaagtgaaa gatgtggccc
      661 agaaggaact gccgggtgag attcttcagg gcacgatttg tcaggattgc catatggagt
      721 catcgacagg cagttccaca tctagacgcg gggcaatgac cagagccatc ggacggcatt
      781 ggtttcgtgg cgtcgtcgct tccggtacca tgctcaagaa tcggaactta caggctgagt
      841 ggatgcctcg gattgatatt gaggtggcga agatcggccc agtggtcgag ggaaccgggg
      901 tcgtgaaggt cggcagtctc ccgcacagtt tccccgatgg cgatccggta ctgaaacaat
      961 tttttctgac cgtcagggtg aaggacgggg cgggcaacac attaacggaa gagacaaaac
     1021 agtttgggtt gccgttcgac aaaatccttc gcggtccgat tcctgatccc ttcatcaagg
     1081 gcggcaacac gaggaaggtc ccattttcgc tgacgctccc ggccggtact gccgctgcat
     1141 cgatagaggc tgtgctgacc tattcgcttc ttcctacgcc cgaacctttc ttgcaggaac
     1201 gataccttgc aactctggaa agcgaaacag gacgggagga ggcgaggagg atcattcagg
     1261 aatatacgca gcggcgcttt ctgacttatc gtgtcaaatc actttcctag cagaatgttg
     1321 aaaaagtcct ccagcatcgt tctcgcatcg ctcagaggct caacgtaccg aagcgtacgc
     1381 ctcgcctcta agacactgga cgctcaccaa ctcgcgcccg tccgcaaacg tgaggctcct
     1441 tcttcgtcgc ctcgcggacc tcgctgggcc ttgcctgtgg aaaggcgcgt cttggcgcgc
     1501 cggggcaggg cgggtgagaa cggaagccct tttgaacatt ctgcgggcga ttctgatttg
     1561 gtgcgtgatc tctctaacag tttgagagtg aaaca
//
LOCUS       DATMTI010000465         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_569407, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000465 DATMTI010000000
VERSION     DATMTI010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569407"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VN039_07525"
     CDS             <1..316
                     /locus_tag="VN039_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45855.1"
                     /translation="GYRKPVAVEGDAKDTLENLRRRADDKPGAIFNPAYDVGARFWFR
                     KAADVIESQAAASARVRQWDRWRDARLFGDEGTPYSNGARDAHKAIVAILDASPEKGG
                     QD"
     gene            659..931
                     /locus_tag="VN039_07530"
     CDS             659..931
                     /locus_tag="VN039_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45856.1"
                     /translation="MTVQQRTMYVAQCDYPRCEVDTSDFDKGNNILYDKPEDLTAAFG
                     YWEGGLDDWGWLLVEGRHYCGDHTVWDDEADKRVPKTAAVVGGESE"
     gene            928..1197
                     /locus_tag="VN039_07535"
     CDS             928..1197
                     /locus_tag="VN039_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45857.1"
                     /translation="MSTQRERVAQIAASALDDDGYHLGVSPAQSMEYWSDDVYRIADA
                     LLPLIREAQAEALIDSRHLVIGTPGFKALTERAAAYRVGEEADHD"
     gene            1437..1610
                     /locus_tag="VN039_07540"
     CDS             1437..1610
                     /locus_tag="VN039_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45858.1"
                     /translation="MGEHEPCEVEYETVEDIGGLLVTTGKRVEVRCGCTETYRYAYGH
                     EDGVPLMWEGVAE"
     gene            1607..2317
                     /locus_tag="VN039_07545"
     CDS             1607..2317
                     /locus_tag="VN039_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45859.1"
                     /translation="MSDLLDAVDLLTKDRFIGTWVPDAAGELKVHTEKHPPLLRLLID
                     GTGITRSAGGSDIKLPIDADALEMVSQIRDRLRLWCRALHVAFDGDELLSTLRRWYLH
                     HVNRVRSGQVSDEVDANITRTVQSWVSQIEGKFEREEKREWQHACVNEIVAPDDDGAL
                     RLRKCGARRIELNGVETFAIELNVTKLTATCRACGGVWAGRQGLAELRFLTNWDQAVR
                     SGEPIDPVAYSLLDTLKG"
BASE COUNT          507 a          571 c          897 g          511 t
ORIGIN      
        1 gggttaccgg aaaccggtgg ccgtggaagg cgacgcaaag gacacgctgg agaatctgcg
       61 tcgacgggcg gacgacaagc caggcgcgat attcaatccc gcgtacgacg ttggggcacg
      121 attctggttc cgcaaggccg ctgacgtgat cgagtcgcaa gccgccgcaa gcgcacgagt
      181 gcggcagtgg gatcgctggc gggatgctcg actgtttggc gacgagggga ctccgtactc
      241 gaacggtgca cgcgacgcac acaaggcgat tgtcgccatt ctcgatgcgt ccccggagaa
      301 gggcgggcag gactgatggc tgaacgaatc ggcatcaggg tgactggtca atctacagac
      361 ccggacggtt ggggtaaggg atatctagtt gaaggcgtcg agtctcccga ttatgagggt
      421 cttgcgccag ttggagatgc ccttatccag atgatcactc aatcggagaa gggcgggcaa
      481 tcatgagcga gtgcacctgc tgggtcaccg acgagaagta ctggtttcag tacggcagtg
      541 cggtcgagcc gggaagtcag atggagtgga acccggattg cccagtgcat ttggcggcgg
      601 ttcctgtctc taccgggagg acagaagaca cgacggcaga ttcagagagg ggtgagtgat
      661 gaccgtgcag cagcggacga tgtacgtggc gcagtgcgac tatcccagat gcgaggtcga
      721 tacgagcgac ttcgacaaag ggaacaacat cttgtacgac aaacctgaag atctgaccgc
      781 agccttcggc tattgggagg gtggccttga tgactggggt tggctgttgg tcgaaggtcg
      841 gcactattgc ggcgaccaca cggtctggga cgacgaagca gataagcgcg tgccgaagac
      901 ggcggcggtt gtgggtggtg agagcgaatg agcacccaac gcgagcgagt ggcgcagatt
      961 gcggcaagcg ccttggatga tgacgggtat cacctggggg tttctccggc tcagagcatg
     1021 gagtactgga gtgacgacgt ttaccggatc gctgacgcct tattgccgct gatccgggaa
     1081 gcgcaggcgg aagcgttgat cgactctagg catctagtga tcggcacgcc gggattcaaa
     1141 gcactgaccg agcgggcggc ggcgtaccgg gttggggaag aagcagacca tgactagcgc
     1201 ccgttgcagc acgttcgttg gccctgaccg gtgtgaacta catgctgggc atggtggggc
     1261 acatcacacc atgtggggga cagtacgaat gacatgggac ggcggcgagg aatggtccca
     1321 cgagctacac acgggcgctg tgcttgccga aacgccgaat gtgggtgtcg atgggggcgg
     1381 acaccagatc gaagctcaga tcggctcaca aggcgttacg gacgaaagcg tgtctgatgg
     1441 gtgagcacga accgtgcgaa gtggaatacg agaccgtcga ggatatcggc ggtcttctcg
     1501 ttactacggg gaaacgtgtc gaggtgcggt gtgggtgtac ggaaacgtac cggtatgcgt
     1561 acgggcatga ggatggtgtg ccgttgatgt gggaaggggt agccgaatga gtgacctgtt
     1621 ggatgctgtg gacttgctca ctaaggaccg gttcattggt acgtgggtgc ctgacgcagc
     1681 cggcgaactg aaagtgcata cggagaagca tccgccgtta ctgcgcctgc tgatcgacgg
     1741 gaccgggatt acacgttctg ctggtggttc ggatatcaaa cttccgattg atgctgatgc
     1801 gctcgagatg gtgtcgcaga ttcgggaccg gctcaggctg tggtgccgtg cgttgcatgt
     1861 ggcgtttgat ggtgacgagt tgttgtccac gctcagacgc tggtacttgc atcatgtgaa
     1921 ccgtgtcaga tctgggcaag tgtcggatga ggttgatgcg aacattactc gcacggttca
     1981 gtcgtgggtg tcacagattg agggcaagtt cgaacgggaa gagaaacgtg aatggcagca
     2041 tgcttgcgtg aatgagattg ttgcgccgga tgatgacggg gcgttgcggt tgcggaagtg
     2101 tggtgcccgc cgtatcgagt tgaacggtgt ggagacgttc gctatcgagc tgaatgtgac
     2161 caagttgacg gcgacgtgtc gtgcgtgtgg tggcgtgtgg gcgggtcggc aggggttggc
     2221 tgagttgagg tttctgacga attgggatca ggccgtccgg tctggggagc caatcgaccc
     2281 ggttgcttac agtttgctgg acacgctgaa gggatgattt gcgttttgtg cgaaagtgtg
     2341 attgaatagg ggaagttgga cagaagtgtg cccggattcg ggtcttccaa ctcggcatcg
     2401 cacctgatcg tttcagattg ggtgcatttt ttgtgccctg atgctcccgg cccggctacc
     2461 cgccgtgtcg gtgagggtgg cgtcga
//
LOCUS       DATMTI010000466         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_17996, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000466 DATMTI010000000
VERSION     DATMTI010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17996"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="murE"
                     /locus_tag="VN039_07550"
     CDS             <1..724
                     /gene="murE"
                     /locus_tag="VN039_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HWV45860.1"
                     /translation="TYALKTKADLRAEAVRLSLQGTTFTAATPVGSFSIESHLVGEHN
                     VYNLLAAIGVALHEGATPAQICEAVARIMNVPGRFERVIAGQPFTVAVDYAHTEDALV
                     RLLMAAQAIKTGRIITVFGCGGDRDRGKRPKMGESAVRYSDVVILTSDNPRTEDPLSI
                     LEQVEVGVIEALRQRPHVQYRKVPDRREAIEEAVREAQSADMVLIAGKGHEDYQIIGT
                     KKVHFDDREVARNAIERLGTRV"
     gene            765..2213
                     /gene="murF"
                     /locus_tag="VN039_07555"
     CDS             765..2213
                     /gene="murF"
                     /locus_tag="VN039_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWV45861.1"
                     /translation="MTLFTVEELREVVGAKILAGEGTGWTRHCIRRISLDTRSLRPGD
                     LFLALRGDRFDGHDFVTTALSRGAVGAIVLDSYDVSRLALKRGSKRTQPFIFGVNDPL
                     YAYQQLAAYHRSRFQIPVVAVTGSNGKTTTKEMVAKVMAHRWKILKTEGNLNNRVGVP
                     QTVLHLNDRHKGAVIEMGVDNLGQTARLCEIARPSIGIITNIGPDHLEFFGTMEVSAQ
                     AKAELLDLLPHDGTAILNADDSYYDYLTARARCRVVSFGFSSKADVRAMDVKSDGRNG
                     TIFRLLLPGTVRHALVHIRVQGDHNVANALAAGAVGSILGLPGGVIAQGLSRFRPAAM
                     RSQVLVNQGVKLIIDCYNANPASMKAAVQLLTQMGAKGKKIAVLGDMLELGPNADQMH
                     EEVGRFLARQGVDLLVACGVLGRSLAKGAKQAGLDPSHIIDAPDARTAAAAMETVVKP
                     GDAVLIKASRGMKLELVADALRGAIRTTKKAS"
     gene            2228..>2556
                     /gene="mraY"
                     /locus_tag="VN039_07560"
     CDS             2228..>2556
                     /gene="mraY"
                     /locus_tag="VN039_07560"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWV45862.1"
                     /translation="MLYIWLYPLHTEFSFLNVFRYQSFRIIYAAVTAFLIAFVLAPWV
                     IRKLQEIKLGQQIRDDGPKRHLAKSGTPTMGGILIIFAVTLSTLLWADISRPHIWLVL
                     AATLGFCA"
BASE COUNT          572 a          681 c          764 g          539 t
ORIGIN      
        1 gacctatgca ttaaagacca aggcggatct gcgtgctgaa gcggtgcggt tgtctcttca
       61 aggaacaacc tttaccgcgg cgactccggt cggaagtttc tccatcgaga gtcatctcgt
      121 gggagagcac aatgtctata acttactcgc tgccatcggg gtcgcgcttc acgaaggagc
      181 cacacctgca cagatttgtg aggctgtggc aaggatcatg aacgtgccgg gacggttcga
      241 acgtgtgata gcgggccagc cgtttacggt ggccgtggat tacgcccaca ccgaggacgc
      301 gctagtccga ttgctaatgg cggcacaagc gataaaaacc ggacgcatca tcacggtctt
      361 tggctgtggt ggagaccgtg accggggaaa gaggccgaag atgggagaat cggccgtgcg
      421 ctacagcgat gtcgtgatat tgacctcgga caaccccaga actgaggacc ccctctcaat
      481 tctggaacaa gtcgaagtcg gggtgatcga agcgctgcgg caacggccac acgtacagta
      541 cagaaaagtg cctgatcggc gagaagccat cgaggaggcg gtgcgggagg ctcagagtgc
      601 ggatatggtt ttgattgccg gtaaagggca cgaagattac cagatcatcg gcacgaaaaa
      661 ggttcatttc gacgatcgcg aggtagcgcg caacgctatc gaacgactcg gaacccgcgt
      721 ctagtccacg ggtgcagggg ccggtcggtg cgcggaagca tcagatgact ctgtttaccg
      781 tcgaagaatt gcgggaagta gtcggcgcga agatcttggc cggtgaaggg accggttgga
      841 caaggcactg tatccggcgg atcagcctag atactcggtc tcttcgacct ggagatctct
      901 ttctcgcact gcgaggagat cgattcgacg gccatgactt tgtgaccaca gcgctatcgc
      961 gcggggcggt gggagcgatc gtgctcgatt cctacgatgt gtcacggctc gccttgaagc
     1021 gtggctcgaa gagaacccag ccatttatct tcggtgtcaa cgatccactc tatgcctacc
     1081 aacagctggc cgcgtaccac cgaagccgat ttcaaatccc tgtcgttgct gtcacgggaa
     1141 gcaacggcaa gacgacgacg aaggaaatgg tcgccaaggt catggcccat cgctggaaga
     1201 tcctcaagac cgaaggcaac ctgaacaacc gtgtgggcgt tccgcagacc gtgctccatc
     1261 tcaacgatcg acacaaagga gcggtcatcg agatgggagt cgataatctc ggccagaccg
     1321 ccaggctgtg tgaaatagcc cgaccctcga tcgggatcat tacaaacatc ggccccgacc
     1381 acttggagtt tttcgggacg atggaggtat cagctcaggc gaaggcggag ttgctcgatc
     1441 ttcttcctca tgatgggacc gcaatcctga atgcagatga ttcgtattac gactatctca
     1501 ccgcgcgagc tcgctgtcgt gtggtatcgt tcggcttttc gtcaaaggcc gatgtccggg
     1561 caatggatgt gaaatccgac ggccgcaatg gaacgatctt tcgtcttctg ctaccaggaa
     1621 cagtacgcca cgcccttgtc catattcgag ttcagggtga tcacaacgtc gcgaatgcct
     1681 tggcagctgg agcggtcggt tctatcctgg gtttacccgg tggggtgatc gctcagggac
     1741 tgtcccgctt ccggcccgct gcgatgcggt cgcaggtatt ggtcaatcag ggcgtgaagt
     1801 tgatcatcga ctgttacaat gctaatcctg cttccatgaa agcggcggtg caattgctga
     1861 cgcaaatggg ggcgaaagga aagaaaattg ccgttcttgg agacatgctg gaactcggcc
     1921 cgaatgccga tcagatgcac gaagaagtcg gcagattctt ggcacgccaa ggcgtcgatc
     1981 tgcttgtggc ttgcggcgta ttagggcgaa gtctcgccaa aggagcgaaa caggcagggc
     2041 tggatccatc ccatattatc gatgcgccgg atgcacgcac cgccgcggcc gccatggaaa
     2101 cggtcgtcaa accgggtgat gctgtgttga tcaaagcctc acgcggcatg aagttagagc
     2161 tggtcgctga cgcactccga ggagcaatac gtacgacgaa aaaggcttcg taacggtcgc
     2221 ccaggctatg ctgtatatct ggctctatcc gctccatacg gagttctcgt ttctgaatgt
     2281 ttttcgttac cagagctttc gcattattta cgccgcggtc acggcgttcc tgatcgcatt
     2341 cgtgcttgcg ccgtgggtga tccgcaagct tcaagaaatc aagttgggac aacagatacg
     2401 tgacgacgga ccaaaacgac atctggccaa aagtggaaca cccacgatgg gcggcatcct
     2461 cattatcttc gcagtcacgc tgtcgacgct gctctgggcc gatatctcac gcccccatat
     2521 ttggctggtt cttgccgcga cgctggggtt ttgcgc
//
LOCUS       DATMTI010000467         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_527350, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000467 DATMTI010000000
VERSION     DATMTI010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527350"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VN039_07565"
     CDS             <1..1557
                     /locus_tag="VN039_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV45863.1"
                     /translation="DTGALMAIMRHDTSGSESVWTASMSVLPSRPLTQSLETDVCIVG
                     AGIAGLSTAYCLSGEGKSVVVLDDGPIGGGMTERTTAHLSNVIDDGYVEIERLHGEPG
                     ARLAAESHAAAIDRIETIVTTERIACDFERVDGYLFHAPEHSEEQLEQERLAALRAGL
                     VGVVRVENPSVPALEPGTCLRFPRQAQFHPLKYLTGLAQAIERHGGQIFTGCHVEAVK
                     GGKPSRIETRSGHVIMAQSVVVATNTPINDMVVVHTKQAPYTTYVIGASVPKHSVPTA
                     LYWDQADPYHYVRIHKGPAADSQDMLIVGGEDHKTGQADDGEDRHLQLEQWARTRFPM
                     IKHIEYRWSGQVMEPIDGLGLIGRNPSDASNVYIATGDSGMGMTHGTIAAILITDLIL
                     GRTNAWTTLYDPSRKSLKAAGTFVQENLNVAAQYGDWLSSGDVESEDHIPKGTGAILR
                     HGIQKIAVYRDEGGELHRCSAVCPHLNCIVAWDSIEHSWNCPCHGSRFNAYGKVLNGP
                     AKADLSPLET"
BASE COUNT          403 a          457 c          482 g          375 t
ORIGIN      
        1 gacacaggag cattaatggc aatcatgaga cacgatacca gcgggagcga atctgtctgg
       61 acggcttcta tgtccgtgct tccctcgcgt ccactgactc aatccttaga aacggatgtc
      121 tgtattgtgg gagccggaat agccggactg tccaccgcct attgtctgtc tggtgaaggc
      181 aaatcggtgg tggtccttga cgatggacca attggtggag ggatgactga acggacgacg
      241 gcccatttgt cgaatgtcat tgacgacggc tatgtggaaa tcgaacgcct gcatggcgag
      301 ccaggcgctc gcctggcagc cgagagccat gctgccgcga tcgatcgtat cgaaacgatt
      361 gtgaccacgg aacgtattgc gtgcgatttt gagcgagtcg acggttacct ctttcacgcg
      421 cctgaacact cagaggaaca actagagcaa gaacgattgg cggcacttcg cgcaggcctc
      481 gtcggtgtcg tgcgggtcga gaatccgtcg gttcccgctc tggaacctgg aacgtgcctt
      541 cgattcccac ggcaggccca attccatccc ttaaagtacc tgaccggtct ggcgcaggcg
      601 attgaacggc acggtggaca gatcttcaca ggctgccatg tcgaggctgt gaaggggggg
      661 aaaccatcgc gaattgaaac tcgatcaggt cacgttatca tggctcagtc ggttgtcgtg
      721 gccacgaaca cccccatcaa tgatatggtc gtggtgcaca ccaaacaggc accctatact
      781 acctatgtca tcggagcgag tgtgccgaaa cattctgtgc caaccgccct gtactgggat
      841 caggctgacc cgtaccacta cgtcagaatc cacaagggac cagcagccga tagccaggat
      901 atgttgatcg tcggtggtga agatcacaag acaggtcagg cggacgatgg agaagaccgc
      961 catctgcaat tggaacagtg ggcccggaca cggtttccta tgatcaagca catcgagtac
     1021 cgatggtcgg gacaggtcat ggaaccgata gatggcctag gattaatcgg tcggaatccc
     1081 agtgatgcat ccaatgtcta catagccacc ggtgattccg gcatgggcat gacgcatggc
     1141 acgatcgccg ccatcctcat caccgatctc attctgggtc gtacgaatgc ctggaccact
     1201 ttgtacgatc cgtctcggaa gtcgctgaaa gcggcgggaa cctttgttca agaaaacctg
     1261 aatgtggccg cgcagtatgg agactggttg tcgtcagggg acgtcgaatc tgaagatcac
     1321 attccgaagg gaacgggggc gatacttcga catgggatcc agaagatagc cgtctatcgg
     1381 gatgaagggg gagagttaca tcgctgctca gccgtgtgcc cgcatctcaa ctgcattgtc
     1441 gcgtgggatt ccatagaaca cagctggaat tgtccctgtc atgggtctcg atttaatgca
     1501 tatggcaaag tactcaatgg accggcgaag gccgatcttt ctccgcttga gacatgactg
     1561 tcggactgat tctctaaagg tggccccccc ctcagagctt ccttaagtat gtacggcttc
     1621 cggttttcac tgaattgcag caaaatccta ccttctagca cgtcctcata tttcaagtgc
     1681 cacatcttcc ctgacagacc gccgaatgac tctagaa
//
LOCUS       DATMTI010000468         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_611714, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000468 DATMTI010000000
VERSION     DATMTI010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611714"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VN039_07570"
     CDS             <1..207
                     /locus_tag="VN039_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45864.1"
                     /translation="WALTDSRPSADVTLIILRVANLAAALPVERRVLACRDGRLRKVA
                     FLTSLRVIVKWIGLILRGWLGQPR"
     gene            complement(190..894)
                     /locus_tag="VN039_07575"
     CDS             complement(190..894)
                     /locus_tag="VN039_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV45865.1"
                     /translation="MADETPKLIQIAPKGGEKKDGFNLVTERVVAVNPESKQLEVELL
                     AYDGKTVVLDVDEEALEDLKKIKVGDGATIRVVEENGKRVAKSFKIRPKDPNAARADA
                     MLLDLRDTHWLNRKYAAEVLGELKDSRAVDPLVAALTDEVGDVRQRAYDSLIKLGGPS
                     VPSLIPLLVSEEDEIRQSATEILRKIGKPAVEPLATALTDADDRLKTRIMKVLDRMGY
                     KPKTKEQPKAELPRLA"
     gene            complement(961..1728)
                     /locus_tag="VN039_07580"
     CDS             complement(961..1728)
                     /locus_tag="VN039_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV45866.1"
                     /translation="MTEPGASDRIEQLIQALHDENEALREHAIASLGQTGPQALSRLI
                     DLMADDDAVIREAASSAVVRMGPSVVDPMIDALQDDSWAIREQAASALGKLRDRRAVE
                     SLVRAIKDRDGAVRTAAVWALERIGDSQAVPGLIEALMDSTLREDAARVLKKIGDVRA
                     VEALIDGLLGSNWMVRRHAAEALGKIGDRRGVTPLIESLKDEDWLVRRNAAESLARLG
                     AKEAIQPLLMLREDENTMVQETVEAVLASLGWTPEPQ"
     gene            complement(1721..2821)
                     /locus_tag="VN039_07585"
     CDS             complement(1721..2821)
                     /locus_tag="VN039_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV45867.1"
                     /translation="MPKETIETLVSELIHEEDWRRMRATAACVAGGPRSVQALIEALQ
                     SGPTELKKEAAAMLARIKDPQAGVALVKLLEHDEEVVRKAGAAALEQMAGVLDTDTAR
                     ALVSLLPKTSEDPERQTLTHLIGAIPTSVLPLCEMLKHRDEDAQVAAALMLDQLLDPR
                     SIDGFIDAMGQPALRDIAVGTLKKLSAIRERIDETFNALREVEGASEREEARMATVID
                     LLGIGRPSVEILIEYLEDDDWLVREAAADLLGKIGDVRAVEPLMKRLEQDKDTGVKEL
                     AIKALGLIGDARPTQLYLAAIPIRPLRVYAMEALAKIKDVGVLRPHKELFDRLRTDRD
                     GLVAYNAGLIADKLEAITAMESQSREEDHEND"
     gene            complement(2856..3956)
                     /locus_tag="VN039_07590"
     CDS             complement(2856..3956)
                     /locus_tag="VN039_07590"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV45868.1"
                     /translation="MTDAVAEQIAALRDEDWAIRGEAAGLLGTFKDPRAVVPLVSLLR
                     DQDRSVREAAIEALRTIGAPAVETVGICLTDPDLSVQESASAILAAIADERVLAPLIR
                     VLSSDDWIVRMHTAKALGRMRNADAVEPLIPLLQDKVKAVREEAAAALAAIGDAAIPS
                     LLKALQHEDWLVRLHAVESLGKAKLERAVEPLLSVLFNDQDSAVREDAVRALGAIGDS
                     QAVEYLFTAMREPGLRTLAVEALGLIGDPRAVPVLIDVLSGTSTPQATRTVAGCGDQW
                     NEEAMTQGAAARALGAIGDDRAIPTLVAALNQTFTRAEAAAALGKFGSKVVPFLLPLL
                     NEPRDENLLFHVRETLASAGWRTGRRSWAGTK"
BASE COUNT          742 a         1301 c         1113 g          890 t
ORIGIN      
        1 tgggcgctca ccgactcgcg cccgtccgca gacgtgacgc tcattattct tcgcgtcgcg
       61 aaccttgctg cggccttgcc tgtggaaagg cgcgtcttgg cgtgccggga cgggcggctg
      121 agaaaggtgg cctttttgac cagcctgcgg gttattgtga aatggattgg tcttatccta
      181 cgaggttggc taggccagcc tcggtaactc ggccttgggt tgctccttgg ttttcggctt
      241 gtaccccatt cgatccagca ccttcatgat tcgggtcttc aaccgatcat cggcatcggt
      301 caacgccgtg gccagcggtt cgaccgccgg tttgccgatc ttccggagaa tctcggttgc
      361 ggattgacga atttcgtcct cttcggacac caggagcgga ataagcgatg gcacggaggg
      421 gccgccgagt ttgatcaacg aatcataggc ccgctgccgc acgtcaccga cttcgtctgt
      481 caatgccgcc accaagggat cgacggcccg cgaatctttt agttcaccca agacctccgc
      541 cgcgtacttc cgattcagcc agtgtgtatc tctcaggtcg agcaacatgg cgtcggccct
      601 ggcggcgttc ggatctttgg ggcggatctt aaagctcttc gcgacccgct taccgttttc
      661 ttccaccact cggatcgtgg cgccgtctcc caccttgatt tttttgaggt cctccagcgc
      721 ctcctcgtcc acgtccagca cgaccgtctt gccgtcgtaa gccaggagtt cgacctcgag
      781 ctgtttgctc tcaggattga ccgccaccac acgttctgtc accaagttga aaccgtcttt
      841 cttctctcca cctttcggag cgatctgaat cagcttcgga gtttcatcag ccatgggcat
      901 gatcctctat tgggttaaat tgaggctcgt cgtttggagg gattttgcac catcctcaaa
      961 ttattgcggt tcaggtgtcc agccaagact ggccagcaca gcctcaacgg tttcctggac
     1021 catggtgttc tcgtcctcgc gcagcatcag caagggctga atcgcttctt ttgcgccgag
     1081 ccgtgcgagt gattcggcgg cattgcgtct gaccagccag tcctcatctt tcagcgactc
     1141 gatcaacggg gtaactcccc ggcgatcgcc gatcttgccc agcgcctctg ccgcatgacg
     1201 gcgaaccatc caattggagc ctaggagacc atcgatcagc gcctcgaccg cccgcacgtc
     1261 accgatcttc ttcaacaccc gagccgcgtc ctcgcgcagg gtgctgtcca tgagggcctc
     1321 gatgagcccg ggcactgctt gagaatcacc gattcgctcg agcgcccata cagccgccgt
     1381 tcgcaccgca ccgtctcgat ccttaatcgc ccggacgaga gactcgacgg cgcgcctgtc
     1441 ccgcaacttg cccaatgccg aggccgcctg ttctcggatc gcccaggagt catcttgcag
     1501 cgcgtcgatc atcgggtcga cgacggaggg gcccatccgc acgaccgcac tggacgccgc
     1561 ctcccgaatc accgcatcgt catcggccat caggtcgatc aaccgggaaa gtgcctgtgg
     1621 gccggtctgg ccgaggctcg cgatcgcgtg ctcgcgcaat gcttcgtttt cgtcgtgcaa
     1681 ggcttgtatg agttgctcga ttcgatcaga tgcacccggt tcagtcattc tcgtggtcct
     1741 cttcgcggga ttggctttcc atggctgtga tggcctcgag cttgtcggcg atcaagccgg
     1801 cattataggc cacaaggccg tcacgatccg ttcggagtcg atcaaaaagt tccttgtgcg
     1861 ggcgcaagac tcccacatcc ttgattttgg ccaaggcctc catggcatac acccgcagtg
     1921 ggcggatggg aatagccgca agatagagct gagtcgggcg cgcatcgccg atcaatccaa
     1981 gggccttgat cgccaactcc ttgaccccgg tatccttgtc ctgttccagc cgcttcatca
     2041 atggttctac ggcccgcaca tccccgattt tccccagtaa gtcggccgcc gcctcgcgca
     2101 cgagccagtc gtcgtcttcg agatactcga tgaggatttc cacgctgggc cgtccgatcc
     2161 caagaaggtc gatgaccgtc gccatgcgcg cttcctcgcg ttcgctggct ccttcaactt
     2221 cccgcaacgc attgaatgtc tcatcgattc gttcacggat tgcactcagc ttcttcaatg
     2281 tcccgacggc gatatcccgc agggccggct ggcccatggc atcgatgaat ccatcgatgg
     2341 aacgaggatc caacaattga tccaacatca atgcggctgc aacctgcgca tcctcgtctc
     2401 gatgtttgag catttcgcat aggggaagga cggatgtggg aatcgccccg atcaggtggg
     2461 tcagggtctg ccgttcagga tcttccgaag ttttcggcag caatgaaacc aatgccctgg
     2521 ccgtatccgt gtcgagcaca cccgccatct gttcgagggc cgctgcgccc gcttttcgga
     2581 cgacctcctc atcatgttcg aggagtttga cgagggcaac tccagcctgt ggatctttga
     2641 ttcgagccag catcgccgcc gcttcttttt ttagttcggt aggcccggac tgcagggcct
     2701 cgatgagcgc ctgaaccgat cgaggaccgc cggccacaca ggcggccgtc gctcgcatac
     2761 ggcgccaatc ctcttcatgg atcagttcgg agaccagtgt ctcaatcgtt tctttcggca
     2821 tgctactcag tagcggaacg gctcatatcc tgagactatt ttgtaccggc ccatgaccgc
     2881 ctgccggttc tccatcctgc cgacgccaag gtctcacgga cgtgaaacag gagattttca
     2941 tcgcgaggct cgttcagcag tggaagcagg aatggaacga ccttcgaccc gaatttgccc
     3001 agtgccgccg ccgcttcagc tctcgtaaag gtttggttca gtgctgcgac gagcgtaggg
     3061 atcgccctgt cgtcaccgat ggcgcccaag gcccgtgccg ctgcgccttg cgtcatggct
     3121 tcttcattcc attggtcccc gcagccggcc accgtcctcg ttgcctgcgg ggtgctggtt
     3181 ccggatagga catcgatcaa cactggaact gcgcgtggat caccgatcag accgagcgct
     3241 tcgaccgcca atgtccgcaa tccaggctca cgcattgccg tgaacaaata ctcgaccgcc
     3301 tgcgaatcac cgatggcgcc caaggcccgt actgcatctt cacgaaccgc agaatcctga
     3361 tcgttgaaca acaccgacag tagcggttcc acagcccgtt ccaatttagc cttccccaac
     3421 gattccaccg catggagacg aacgagccag tcctcatgct gcaacgcttt caagagggac
     3481 ggaattgccg cgtcgccgat ggcggccagg gcggcagccg cttcctcacg aaccgccttg
     3541 accttgtctt gaagcagtgg aatgagcggc tcaactgcgt cggcgttccg catccgcccc
     3601 agcgccttgg ctgtgtgcat ccgaacgatc cagtcgtcgc tggacagtac cctgatgagc
     3661 ggagcaagca cccgctcatc agcaatggcg gccaagattg ccgacgctga ttcctggacc
     3721 gagagatcag gatccgtgag gcagattccc accgtctcga cggccggcgc tccgatcgta
     3781 cgcaacgctt caatcgccgc ctctcgcacg gaacggtcct ggtcccgaag gagagaaacg
     3841 agaggaacca cagcacgggg gtctttgaat gtaccgagca ggccggccgc ttcaccacgg
     3901 atcgcccaat cctcgtccct gagtgccgcg atctgctctg cgacggcatc ggtcatgctg
     3961 ttgtccattc ttgcaggatt cagcggctca cttcagaata aatgtcttca cggcgaccgg
     4021 acggcggctg tgaacctagc tgcagg
//
LOCUS       DATMTI010000469         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_59983, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000469 DATMTI010000000
VERSION     DATMTI010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59983"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1974
                     /gene="asnB"
                     /locus_tag="VN039_07595"
     CDS             178..1974
                     /gene="asnB"
                     /locus_tag="VN039_07595"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWV45869.1"
                     /translation="MCGIAGYCSFEAGDLLGKEIQQKILDLMKTRGPDGSGVWTDAGD
                     RVTLLHRRLAIQDLSETGAQPMLSDDGRLAITFNGEIYNVAELRRWVPDYPFKGTSDT
                     EVLLAIYQKFGQQMLKYLRGMYAFAIWDDRNQGLFLARDAYGIKPLYLATTPHGLWFC
                     SQVKPLLEVPGVDLSPEPAAHAGFFLWGSVPEPYTLYRGIRSLRSGHSLWLQRGGEPK
                     DIQFASIAELMAQSAPLSPGQSESERLQDALRESVRAHFVADTPVAVFLSAGLDSSTI
                     LGVAAGSFCPANLNCLTLGFDAFQNTGCDERAEATHIASHYGVPQHIQVVTQADFEAQ
                     STHFLEAMDQPTIDGINTYFVARMARDAGFKVALSGIGGDELFGGYGSFRQIPQMMNL
                     ARAMGGSRRAGRFFREHTEPLFQRVTSPKYAGVLEYGRRIEGAYLLRRSLFMPWELSK
                     VLDADLADAGLAELERAECEDAELEKLKGLPLHLQISFLESTRYMRNQLLRDADWAGM
                     AHSLEIRTPLVDQFLLKQLSPLLSSKHPPTKLEMAATPNKPLPQSVLHRKKTGFSVPV
                     REWLTSSLADKPERGLRSWAKFVYRHQYPKAS"
     gene            2125..>2346
                     /locus_tag="VN039_07600"
     CDS             2125..>2346
                     /locus_tag="VN039_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45870.1"
                     /translation="MRMTIGLLALATFAATPVFAKPSNKLVVHIKTSAGQDAGTATFR
                     QLKDGKLSIKLNLKNLPFGEHAVHIHEHPV"
BASE COUNT          519 a          678 c          654 g          495 t
ORIGIN      
        1 ctgcgaaggt agatgaacaa tctaacgatc ttgatacaga tgagtcttaa taattgctta
       61 aggtatgagg acaacaatca agtcgaaatc ggccccccgg agaacagagg agaagaggcg
      121 atccggcacc tggatgactt acccgctcaa cgggagcatc cctcacgata ggcatatatg
      181 tgcggcatag ccggctattg cagttttgaa gcaggagacc tcttaggcaa agaaatacag
      241 caaaagatcc tcgacttgat gaaaaccaga ggtcccgacg gaagcggcgt ctggacggat
      301 gcgggagacc gagtcacgct gctgcatcgg cgtttggcca tacaggactt gagcgagacg
      361 ggagcccaac ccatgctctc ggacgacggt cgcctggcca ttacgttcaa cggcgaaatc
      421 tacaatgtcg ccgagctgcg acggtgggtt cctgattatc cgttcaaggg aacctccgac
      481 accgaagtgc tccttgcgat ttatcaaaaa tttgggcagc agatgctcaa gtacctgcgt
      541 ggcatgtacg cctttgccat ctgggatgat cgtaaccagg ggctcttttt agctcgcgac
      601 gcttacggca tcaagcctct gtatctggcg acgacgcccc acggattgtg gttttgttcg
      661 caggtgaagc ctctgctcga agttcccgga gtggatctct cgcctgaacc ggccgcgcac
      721 gccggttttt ttctatgggg cagcgttccg gaaccgtata cgctttatcg cggcattcga
      781 tcgcttcgca gcggccattc gctctggctg caacgtggag gcgaaccgaa ggacatccag
      841 tttgcctcca tcgccgaact tatggctcaa agcgcgccat tgtcaccggg ccaatccgag
      901 tccgaacgac ttcaggacgc gcttcgcgaa agcgttcgcg cgcacttcgt cgccgacacg
      961 ccagttgctg tctttttatc ggctggactg gactcatcga cgatcctggg agttgcagcc
     1021 ggaagttttt gcccggcaaa cctgaattgc ctgaccctcg gcttcgacgc ctttcaaaat
     1081 accggatgcg atgaaagagc cgaggccact cacatcgcga gccactatgg agttccgcaa
     1141 cacattcaag tggtgacgca ggccgacttc gaggcacaat ccacccattt ccttgaagct
     1201 atggaccagc caacgattga cggaatcaac acctacttcg tcgccaggat ggctcgggac
     1261 gcgggattca aggttgcgct ctcggggatt ggcggcgatg agctctttgg gggatacggc
     1321 agctttcggc agattcccca gatgatgaac ctcgcgcgcg ccatgggagg atctcgcagg
     1381 gctggcaggt tcttccgcga gcacacggaa cctctttttc agagggtcac ctctccgaaa
     1441 tatgccggcg ttctagagta cggccgtcgc atcgagggag cgtacctgct ccgcagaagc
     1501 ctgtttatgc cgtgggagct ttcgaaggtt cttgatgcgg accttgcgga tgcgggcctc
     1561 gcagagttgg agcgcgcgga gtgcgaagat gcagaactcg aaaagttgaa gggccttccc
     1621 ctgcatcttc aaatctcttt cctcgagagc acacgctaca tgcgcaacca gcttctccgc
     1681 gacgccgatt gggcaggcat ggcgcattcg ctcgagatca gaacgcccct ggtcgatcag
     1741 tttctgttga agcaactgtc gccgctgctg tcttcgaaac atcctccgac gaagttggag
     1801 atggcagcaa ctcccaacaa gccattgcct cagtcagtcc ttcatcgcaa aaagactgga
     1861 tttagcgtgc cggttcggga atggctcacc tcttctcttg ccgacaagcc ggaacgaggt
     1921 ctgcgctcgt gggcaaagtt tgtctatcgt caccaatatc cgaaggccag ttgagaacgc
     1981 tgaatgccgt cctcgcagag atggacgaga ggagttcccg gttgatgaca cttgccggtg
     2041 ttgcctgttg cgagaacgag ggataccttt ctacttgcag gagtttccag cccgcctgaa
     2101 agctacctta agggagacag caccatgcgc atgaccatcg gcctactggc cttagctacc
     2161 tttgccgcca cacctgtatt cgccaagccc agcaacaagc tggtcgtgca catcaagacc
     2221 agcgcggggc aggatgccgg aacggccacc ttccgccagc tcaaggacgg caagctttcg
     2281 atcaagctga acctcaagaa cctccccttc ggcgagcacg cggtccacat ccacgaacac
     2341 ccggtc
//
LOCUS       DATMTI010000470         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_59989, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000470 DATMTI010000000
VERSION     DATMTI010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59989"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VN039_07605"
     CDS             <1..340
                     /locus_tag="VN039_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV45871.1"
                     /translation="WNLIGNAIKFTPPGGRVSVEFGESPRGVVQTCVADTGCGIDASH
                     VPNIFDEFSRVPSPMPAAQGAQLGLCITKTLVTMHHGRIWVESQSEVGSRFYFTLPVA
                     GSQDEPGRTM"
     gene            405..1850
                     /locus_tag="VN039_07610"
     CDS             405..1850
                     /locus_tag="VN039_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV45872.1"
                     /translation="MRAKILVVDDDPDILLSLQNRVSFMGHEPLTATNGKDALRLIGE
                     EAPDLVLLDLKIPDITGLEVLKQVAAASIPSQLKGEETTQTTPYTTPLIVILTAYGTI
                     ELAVQAMQLGAFDFVPKPFTADHLTVVINKALDTVALHRQVETLRKEVDAQFEPIVST
                     SQRMSDQLTVAKQAAASSATVLLLGETGTGKEVVARAIHRWSPRSAQPFIAVNCAAFP
                     ENLLENELFGHEKGAFTGAAKREPGKIEIAEGGTLFLDEIGDMPLTMQSHLLRVLQDR
                     TFYRVGGTQEVRTDVRFIAATNKDLNQAIRQGTFREDLYFRLAVITIALPPLRERMED
                     LSVLVAHVLNRPGNVGLYKCLTLSDEAFHALQRYPWPGNVRELENVLTRALILCPGDT
                     IGPEHLSLPALTTPLTSPPSGTQEPNTDILFFSYHQSMDVYSQKLIEAALRRNGWNQT
                     KAAAELGLQRTYLTKLLRQKQIPSKPLTPPE"
BASE COUNT          489 a          618 c          566 g          447 t
ORIGIN      
        1 atggaatctc atcgggaatg ccatcaaatt cacgccaccg ggaggtcgag tgagcgtgga
       61 gtttggcgaa tcgccgcgtg gagtcgtcca aacttgcgtt gccgataccg gatgcgggat
      121 tgatgcgtcc catgttccaa atatttttga tgagttttcc agagtaccgt cgcccatgcc
      181 ggcagcacaa ggcgctcagc ttgggctctg catcaccaag acactcgtca ccatgcacca
      241 tggccggata tgggtggaaa gccaatcaga agttggatca cgcttttact tcacgctgcc
      301 ggttgccggg tcacaggatg aaccgggtcg gacaatgtaa cgagtttgcc gcttcaagat
      361 aggtttgcga gaagagttgg atattcggaa aggagagaca ttctatgcgc gctaaaattc
      421 ttgttgtcga tgacgacccc gatatcctcc tgagcttgca gaatcgtgtc agtttcatgg
      481 gacacgaacc cctgacggcg acgaacggca aggatgcctt gcgcttgatc ggggaagagg
      541 caccggatct tgtcttgctg gatttgaaaa tcccggatat caccgggctt gaggtgttaa
      601 agcaagtcgc tgcggcatca attccgagcc aacttaaagg tgaggagaca acccagacaa
      661 ctccttacac cactccgctg atcgtcatac tcaccgccta tggaacgatc gaactggcgg
      721 tgcaggccat gcaactcggc gccttcgatt ttgtgccgaa gccgtttact gcggaccact
      781 tgaccgtcgt gatcaacaag gccttggaca ccgtcgccct ccaccgtcag gtcgagacgc
      841 tccgcaaaga agtcgatgcg cagttcgagc cgatcgtctc caccagtcag cggatgagcg
      901 accaactgac tgtggccaaa caggccgcgg catcctccgc gaccgtctta ttgctcggcg
      961 aaaccgggac ggggaaagaa gtggttgccc gcgccattca tcgttggagc cctcgctcgg
     1021 cgcaaccctt tattgccgtc aattgcgccg ccttccctga aaatctattg gagaacgaac
     1081 tctttggaca cgaaaagggc gcgttcaccg gagccgccaa acgggagccg ggcaagatcg
     1141 agatcgccga gggaggcacg ctgtttctgg acgagatcgg cgacatgccg ctcaccatgc
     1201 aaagccatct cttgcgggtc cttcaagacc ggaccttcta ccgtgtcggc ggcactcaag
     1261 aagtacggac cgacgtacgc tttatcgccg ccacgaacaa agatctgaac caggcgattc
     1321 gtcaaggcac gttccgcgaa gacctgtact ttcgtctcgc cgtgattacc atcgccctgc
     1381 ccccgttgcg tgaacggatg gaggatctgt ccgtcctcgt ggcacatgtc ctcaaccggc
     1441 ccggcaacgt cggcctgtac aaatgcttga cccttagcga cgaagccttc cacgcactgc
     1501 aacgctaccc ctggccgggc aacgtgcggg aattggaaaa cgtgctcact cgagccttga
     1561 tcttatgccc cggtgatacg atcggaccgg aacacctctc actgcccgct ctcacgactc
     1621 cactgacctc ccctccctcg ggtactcagg agcccaacac cgacatactg tttttctcgt
     1681 atcatcagag catggacgtc tatagccaga agctgatcga ggccgcttta cgtcggaatg
     1741 ggtggaatca gaccaaagcg gcagccgaac tcggtctgca acggacctac ttgaccaaat
     1801 tgctccggca gaagcagatt ccaagcaagc cccttactcc cccggagtga aggaatgggc
     1861 ggcactcttc aaaaggtttt gacctcgtag ttcggcctct ccaaatagcc gcaaacggca
     1921 tcggccgtcc ttttcccgaa ttgatccgca tccaattaga tccagcctgg aatccattct
     1981 gattcagaag caagccgcca cactatgagt acatctgcgt aggtgctgaa aataccatga
     2041 gttagatttg gatcctgtgt ggcttaacaa atgcatagtg cttgtcatga aggaaggctg
     2101 gaggatcctc gatgcagaac
//
LOCUS       DATMTI010000471         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_485162, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000471 DATMTI010000000
VERSION     DATMTI010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485162"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..385)
                     /locus_tag="VN039_07615"
     CDS             complement(38..385)
                     /locus_tag="VN039_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWV45873.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAETKGNHVGDGQGI
                     GDNIYVIAHDRCTECVGHFDEPQCAAVCPVDNCCISDPLYPETTDVLLERAKTLNPDK
                     AIDAAKVWSGVRN"
     gene            612..1112
                     /locus_tag="VN039_07620"
     CDS             612..1112
                     /locus_tag="VN039_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWV45874.1"
                     /translation="MASDQAQASNREQRKKRIIMMILLAFLLMSASVFLVERKESSII
                     VITFPSGIELEAEVADTPEKLLFGLAFRDALPPNGGMLYIFEQNGMHRVTTKEYRFPI
                     DILWVDESHHVVHMLEHAEPCAQDPCPLLGPPLEAVRYVIQAESGFIKKAGVAKSDEL
                     KYALRM"
     gene            1144..1461
                     /locus_tag="VN039_07625"
     CDS             1144..1461
                     /locus_tag="VN039_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWV45875.1"
                     /translation="MDEFQKVAQLDEVPPGKSKIVKINDRSIALFNVEGKLYAIHNSC
                     PHEGAPLIEGRLKGYVVACPWHDLAFDIRNGQGIDGGGYCVGSYEVRVDGNDILIGPR
                     RKL"
     gene            1515..1853
                     /locus_tag="VN039_07630"
     CDS             1515..1853
                     /locus_tag="VN039_07630"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HWV45876.1"
                     /translation="MSISRADHNPAMDGSDCRKIMVDQSFFIHLWEDRTRGEQWVPSY
                     DTKGLALLSDEFLRVASNNAVENGQRIFEFKAVQSGVHQLIFEKRMGWKFTAEDRRLF
                     RIEAEPPSRN"
     gene            1858..>2665
                     /gene="dat"
                     /locus_tag="VN039_07635"
     CDS             1858..>2665
                     /gene="dat"
                     /locus_tag="VN039_07635"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HWV45877.1"
                     /translation="MPDIAFINGRFLSWEEATVSIDDRGFQFGDAVYEVIRTYRGSPF
                     ELAAHLARLDRSARELSLRQPYTSVQWTRWIQQGLSLAGYQDAKVYIQLTRGVAPREH
                     SFPSDILPTVVMTIRRFHPLAPEVRLAGVSACTREDLRWGRCDIKSVNLLANVLAREE
                     AKKAGVFETILVRDGFVMEGALSNVMAVQDGVVITAPEGSRILSGVTRTVVLELAKKD
                     DIVIEERFIPVDLLYRADEIFLTGTTLEVLGVVQIDGKTIGSGQPGLITKM"
BASE COUNT          656 a          666 c          728 g          615 t
ORIGIN      
        1 gacagaacca gggcccttaa agaagtgcac tagagaatca gtttcttacc ccactccata
       61 ctttggcggc atcgatcgct ttatcgggat tcaaagtctt cgccctttcc agaagcacat
      121 ccgtcgtttc aggatagaga gggtctgaaa tacagcaatt atcaacgggg caaaccgcag
      181 cacactgagg ttcatcaaag tggccgacac actcggtgca gcggtcatgc gcgattacat
      241 aaatattatc gccgatgccc tgcccatcgc cgacgtgatt gcctttggtc tcagcatcgc
      301 ttctggtttc aaagattgct tcattcggac attccggcag gcatgctcca caagagatgc
      361 attcatccgt aatcaataaa gccatgacct aagcctcctt atcacgtcaa aagactataa
      421 gaacattgac aagtccacgc ggctcacacc atagtgcctc ggtggcttat ggcgagtaac
      481 aacgcggaaa tgcattctat tctgtgattc ttttctaagt caactgaacg gaatccggtc
      541 agaagaccta gaccattatt gtgtatgggc cattgggccc aacagagaag aaagcgccgg
      601 tcgtcatcag catggcatct gatcaagctc aggcgagtaa tagagaacag cggaaaaagc
      661 ggatcatcat gatgatcctg ctcgcctttc tcctgatgag tgcttcggtt ttcctcgtcg
      721 aacgcaagga atcgagcatc atcgtcatta cgttcccgag cggcattgag ctggaagccg
      781 aagtggcgga cacgccggag aagctgctct ttgggcttgc ctttcgcgat gcattgcctc
      841 caaacggcgg catgctttat atctttgagc aaaacgggat gcatcgcgtg acgaccaagg
      901 aatatcgatt ccccatcgac attctgtggg tggatgagag tcatcatgtg gtgcatatgc
      961 tggaacatgc agagccatgt gctcaagatc cttgccccct tttggggcca ccactcgaag
     1021 ctgttcggta tgtgatacag gcagaatcgg ggttcattaa aaaagcggga gttgcaaaaa
     1081 gcgacgagct caagtatgcc cttcgcatgt agaggttggg aaacagcaag aggaatacgc
     1141 acgatggatg agtttcaaaa ggttgcacag ctcgatgagg taccaccggg aaagtcaaag
     1201 atcgtcaaga tcaatgaccg ctcgatcgcg ttgttcaacg tcgagggaaa gttgtatgcc
     1261 atccataata gctgccctca cgagggtgct ccgcttattg aaggaagact caaagggtat
     1321 gtcgtggcct gtccctggca tgacctggcg ttcgatatca gaaatgggca aggtatcgac
     1381 ggcggcgggt attgtgtagg aagttacgaa gtccgggtgg acgggaacga tattctgatc
     1441 ggccctcggc ggaaactata aaaagggaga tccggctgaa cggttgtctt cacgacgcgg
     1501 atcagggtat cgacgtgagt atttccaggg ccgatcacaa ccctgctatg gacggcagcg
     1561 actgtcgcaa gatcatggtc gaccagtcat tcttcatcca tttatgggaa gatcgcacca
     1621 gaggtgagca gtgggtgccg tcttacgaca ccaaaggact tgcgcttctc agtgatgagt
     1681 tcctccgtgt cgcgagcaat aacgccgtgg aaaacgggca gcgcatcttc gagtttaagg
     1741 ccgtgcagtc aggcgttcat cagctgattt tcgaaaaacg catgggatgg aaatttaccg
     1801 ctgaagatcg gcgcttgttc agaattgaag cagagccacc atccaggaat tgatctcatg
     1861 cccgacattg cgttcatcaa cggccgcttt ctttcttggg aggaggcgac cgtttctatc
     1921 gatgatcgag ggtttcaatt tggagatgcc gtctacgaag tcattcggac ctatcgaggg
     1981 agcccgttcg agcttgccgc gcatcttgcc cggttggatc ggagtgcaag agagctctcg
     2041 cttcgccaac cctatacgag cgttcagtgg acgcggtgga ttcaacaagg actcagtctg
     2101 gccggatatc aggatgccaa ggtctatatc cagcttacca gaggggtggc tccccgtgag
     2161 catagtttcc cctccgatat tctcccgacg gttgtcatga ccatcagaag gtttcatccc
     2221 ttggcgccag aagtccgcct ggccggcgtc agcgcatgta ccagggagga tcttcgatgg
     2281 ggacgctgtg atatcaaaag tgtcaatctc ctggccaacg tccttgcccg tgaagaagcg
     2341 aagaaggccg gtgtcttcga aaccattttg gtcagagacg gcttcgtcat ggaaggtgcg
     2401 ctgagcaatg tcatggctgt tcaagatgga gtcgtcatca cggctcccga aggttctcga
     2461 atcctatccg gcgtcacacg gactgtggtt ttagaactgg cgaagaagga tgacatcgtg
     2521 atcgaggaac gattcatacc ggtcgacctc ctgtaccgcg cggacgagat atttttgacc
     2581 ggaaccacgc ttgaagtact cggggttgtc cagatcgatg ggaagaccat cggctccggc
     2641 caaccaggtc ttatcacaaa aatgc
//
LOCUS       DATMTI010000472         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_145448, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000472 DATMTI010000000
VERSION     DATMTI010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145448"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /gene="ccmI"
                     /locus_tag="VN039_07640"
     CDS             complement(<1..761)
                     /gene="ccmI"
                     /locus_tag="VN039_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HWV45878.1"
                     /translation="MTATFWLIVSAMTLCVLGLLLRPLLKRPSAASSDQEKTLPVYRQ
                     QFSELEQDRANGLLTEEQYRTARSELERRVLEEAGTPEAQTSVVGGLVNLRFVALSLA
                     MIIPAASGVLYWTLGNPAAMTHPNVSSVASQGGPGDDSQMADSLSTLIEQLRKRLEQN
                     PNDAVGWGLLARSYMAMERYADAVPIFEKATKLNPNNASLLADYADALGVHQGRKLDG
                     KPETLIQKALKLDPHNVKALMLSGTIAYNRKDFARA"
     gene            complement(758..1219)
                     /locus_tag="VN039_07645"
     CDS             complement(758..1219)
                     /locus_tag="VN039_07645"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HWV45879.1"
                     /translation="MNWLILIALLWSGPAWAGEARPLADNPAAEARLKHLAVELRCLV
                     CQNQTLADSNAPLAEDLRREVREMIAKDMSDKEIIDFLVARYGDFVLYRPPLKATTTL
                     LWVGPFVLLTIGATALIITLRRRAHKVVDVPVTDEEHRRVEQLLAEGGKRS"
     gene            complement(1216..1743)
                     /locus_tag="VN039_07650"
     CDS             complement(1216..1743)
                     /locus_tag="VN039_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HWV45880.1"
                     /translation="MNRFLLPLSIFIVVVGFLGVGLKLNPREIPSPLVGKAAPDFSQP
                     QLYDPANMFSTADLKGKVWLLNFWASWCSGCRTEHPVLMELAKSGEVPIYGMDYKDQH
                     DEAMAWLKRWGNPYPVVGVDDAGRVGINYGVYGVPETYVIDKQGVIRYKQIGPLDPDT
                     VAKKILPLVKQLESQ"
     gene            complement(1740..>2070)
                     /locus_tag="VN039_07655"
     CDS             complement(1740..>2070)
                     /locus_tag="VN039_07655"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HWV45881.1"
                     /translation="FRVSLDGRETTTLYPEKRRYAVQNQVMTEAAIDPGLLRDLYVSL
                     GEPLNDGAWSVRLYHKPFVDWIWGGCFIMALGGALAISDRRYRIAWRKQEPAVPASTR
                     TARRKVA"
BASE COUNT          462 a          641 c          526 g          441 t
ORIGIN      
        1 gcacgcgcaa aatcttttcg gttgtacgcg atcgtccctg acaacatgag cgccttcacg
       61 ttgtgtggat cgagctttaa ggccttttgg atcagcgtct cgggctttcc atccaacttg
      121 cgcccctgat gcacaccgag agcatccgca tagtcggcaa ggaggctcgc attgttggga
      181 ttgagcttgg tggccttctc gaaaatcggc accgcatcgg cataccgctc catggccata
      241 taggatcgag ccaagagtcc ccacccgact gcatcgttgg gattctgctc gagccttttc
      301 ctcaactgtt cgatcaaggt gctcaggctg tccgccattt gagaatcatc tccgggaccg
      361 ccctgagagg caaccgatga tacattcggg tgcgtcatcg cagccggatt tcccaatgtc
      421 cagtagagca caccactggc agccggtatg atcatggcca aggaaagggc aacgaaccgg
      481 agattcacca gccctccgac aactgaggtc tgcgcctccg gcgtccccgc ttcctccaac
      541 acacggcgct ccaactcgct ccgagccgtt cggtactgtt cctccgtcag caatccattg
      601 gcacgatctt gttccagttc cgaaaactgc tgtcgataga ccggcagcgt tttctcctga
      661 tcgctcgagg cggctgacgg gcgcttcaac agaggtctca acagaagacc gaggacacac
      721 agcgtcatgg ccgaaacgat caaccaaaat gtagcggtca tgagcgcttg cctccttctg
      781 ccaatagttg ttcgactcgt cgatgctctt catccgtcac cggcacatcg acgactttgt
      841 gtgcccgacg ccgcaacgta atgatcagcg ccgtggctcc gatggtcaat aggacaaacg
      901 gacccaccca cagcaatgtg gtcgtggcct ttaacggcgg acgatacagc acgaaatcgc
      961 cgtagcgcgc gacaagaaaa tcgatgattt ccttgtcgct catgtctttg gcgatcattt
     1021 cccgaacttc tcgacgcaag tcctctgcca gtggggcgtt ggaatcggcc aatgtttgat
     1081 tctgacagac cagacatcgc agctcaacgg cgaggtgctt gagtcgcgcc tcagcggcag
     1141 gattatctgc caacggcctg gcttccccag cccaagccgg tccggaccag agcagcgcta
     1201 tgagtatcag ccaattcatt gtgattcgag ctgctttaca aggggaagaa tcttcttggc
     1261 gacggtgtcg ggatccaatg gcccgatctg tttataacgg atcacgccct gcttgtcgat
     1321 gacgtaggtt tcagggactc cataaacccc ataattgatg ccgacccggc cggcatcatc
     1381 caccccgact actggatagg gattgcccca ccgtttcaac catgccatgg cttcgtcatg
     1441 ctgatccttg taatccatcc catagatcgg cacctcgccg gatttggcca actccatcag
     1501 caccggatgt tcagtccgac atccgctgca ccaggaggcc caaaaattga gcaaccacac
     1561 tttccccttg aggtctgccg tcgaaaacat gtttgctgga tcatagagct gcggctggga
     1621 aaaatcaggg gcggcctttc cgaccaacgg agatgggatt tcccgaggat tgagcttaag
     1681 ccccaccccc agaaaaccca cgacaacgat gaaaatcgac agcggcagaa gaaaccgatt
     1741 catgccactt ttcgcctcgc ggttcgtgtt gacgcaggca cggccggttc ttgcttgcgc
     1801 cacgcgatcc gatacctgcg atcgctgatg gccagtgcgc cgcccagcgc catgatgaaa
     1861 cacccgcccc aaatccaatc cacaaatggc ttgtgataaa gccgcacact ccacgctcca
     1921 tcgttcagcg gttcccccaa cgacacatac aggtcgcgca agagccccgg atcaatggcg
     1981 gcttcggtca tgacttgatt ctgaacggca taacgtcgct tctcaggata caaggtcgtc
     2041 gtttcacgac cgtccaggct cacacgaaag
//
LOCUS       DATMTI010000473         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_739266, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000473 DATMTI010000000
VERSION     DATMTI010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739266"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..464
                     /locus_tag="VN039_07660"
     CDS             231..464
                     /locus_tag="VN039_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45882.1"
                     /translation="MDTKIFEFNDLQVWDRGGRYFVRYDAGAHQVVVREDEISEAEAR
                     EAAKGKDEALKMLFALRKRLIQSRVDPYVPNIE"
     gene            617..766
                     /locus_tag="VN039_07665"
                     /pseudo
     CDS             617..766
                     /locus_tag="VN039_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(833..1273)
                     /locus_tag="VN039_07670"
     CDS             complement(833..1273)
                     /locus_tag="VN039_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45883.1"
                     /translation="MRWKFIGLITAAILAMALALYGATLWKLHVRRASNPPPHLTPLL
                     TGYAASTNLPVDPILHCVMSQKDLRMADKPLSKGFVDHLMQGNADWTAISERMQAGDV
                     IYAYSHEAQVPPGMSRPDAFTGISSGYVVLRGRCLIGMIGTMVE"
     gene            complement(1358..>1624)
                     /locus_tag="VN039_07675"
     CDS             complement(1358..>1624)
                     /locus_tag="VN039_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45884.1"
                     /translation="ISGYNSGERPSDDFSPFIMKRLTMRGFLVLDYTKTREAVGAITG
                     WIRQGKLKTEETVADGLENAPVVLNRLFDGSHRGKLVLRVDAAT"
BASE COUNT          350 a          433 c          448 g          393 t
ORIGIN      
        1 caagcgccaa gcggggtggc ctttctcttt ggttatcttt ctctttggcc acgcaaagag
       61 aaagtaactc gcgcgccgaa ggcgcacgaa agctcttgct tctgatttct catgcggtaa
      121 ggctaatcta tcagtgggaa ctgagcctga ccccgatatc gtcctggaca tcgatatcct
      181 gtgccgccta gcgcttatac agtcacccca gtttttccgt gaggaatttt atggatacga
      241 agatttttga gttcaatgac cttcaggttt gggatagggg tggccgatac ttcgtgcgtt
      301 acgacgccgg ggcacatcag gttgtggtgc gagaggacga gatctcagaa gctgaggcta
      361 gggaggcggc aaaggggaag gacgaagcct taaagatgct gtttgcattg cgaaagcgtt
      421 tgatccaatc tcgcgtcgac ccatacgttc caaacattga atgatgaaat aagcggtgcc
      481 caccatgtgc gggcgtttct ttgttccaaa cagagagcag catgcggtca gatatagctt
      541 cgtagcattg ctggagtagc atccaacgta tataactcga ccctttcgcc tcgaattcgg
      601 tggtgcactc taccatgtga cggcgcgtgg agatcgccgc ggagtgatct atgaagatga
      661 cgtggatcgc gagcattttc ttgacgtgct tggtcaggtg gtcgaacggt tcaattggcg
      721 ttgccatgcg tactgcctga tgaccaatca ctaccacttg gtgatcgcga cgccggatgg
      781 caatttgtcg aaaggaacga aaaaggcgcc agcgacaatt tctggatatc ggtcattcga
      841 ccatggtgcc gatcatgccg atcaggcagc gccccctcag caccacatag ccactgctga
      901 ttccggtgaa agcgtcggga cggctcattc ctggcggtac ctgtgcctcg tggctgtacg
      961 cataaatcac gtcgccggct tgcatgcgct cgcttatggc ggtccagtct gcatttcctt
     1021 gcatgaggtg atccacaaag cctttgctca aaggcttgtc tgccatccgc agatcctttt
     1081 gtgacatcac gcagtggagg atcgggtcga ccggcaaatt agtgcttgcc gcatatcccg
     1141 tgaggagtgg cgtcaagtgt ggtggtggat tcgacgctcg acgaacatgc aacttccaca
     1201 gggttgcgcc gtacaaagca agcgccatcg caaggatggc tgctgtaatc aacccgatga
     1261 atttccatct catgactgtg taatttcctg gcttgacggt agcctcgcac gacatgttgc
     1321 gagatcgact ggaatgcgta ttcaagttac ttgaatctca tgtcgccgca tccacccgca
     1381 acaccagctt ccctcgatga ctgccgtcga acaaccggtt gagcaccacc ggtgcattct
     1441 ccaacccatc ggccacggtc tcctcggtct tcagtttccc ctggcgaatc cacccggtga
     1501 tggcacccac cgcttcgcga gtcttcgtgt aatccagcac cagaaatccg cgcatggtca
     1561 gtcgtttcat gatgaagggg ctgaagtcgt cgctggggcg ttcgccgctg ttgtagccgg
     1621 agat
//
LOCUS       DATMTI010000474         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_315578, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000474 DATMTI010000000
VERSION     DATMTI010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315578"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VN039_07680"
     CDS             <1..969
                     /locus_tag="VN039_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWV45885.1"
                     /translation="IAVMSSTTFSATDYPTAVKANSPAGYYRLGETSGTIAASEVNSP
                     TVDGTYTITTGQLNYESGISDSTNGSFYCGTGDFVIAPNNSLFNFGDTFSLEAWYKPT
                     GIFSGQFRVIATKGGTSWQFGTNTLGQVRLIGGPSQSTLVATSTASLTVGVWHHIVVT
                     KSGSTTKIYINGIEGTTAGTNVTFVNNTNGIEIGGSSLQAIDEVAFYPTALSASDVQA
                     HYKAGSVPGAPSNKRAPKLEGTTVVGDQVRCLTGRWLAAPTSFSFQWQSAATSGGTYA
                     NITGATSSTYTLTSGESTKYVRCQVTASTVGGTSSAVSSNVLGPVS"
     gene            1129..>1911
                     /locus_tag="VN039_07685"
     CDS             1129..>1911
                     /locus_tag="VN039_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45886.1"
                     /translation="MALTAGFNPAGAVSGNSILATDAGDATAWNSVSTVSGWPKYSNS
                     IALFGKTLSARVATAGTAPSLSWSTSYASPAEDYGRLYLYINTLPSAAGAPLVFGNVI
                     NGFSIVLTSAGKITINKSTGAAQATSATTLSALQWYRIEYHYATGASTPSVSIYTSPD
                     SASAAETLTASSWTVVAAPSILFGGDGNTKTFDLNVAQIAVLGTAAIGPYPVNSIAPA
                     VSGSAPAGSTLTAANGTWNGTFNYTYQWTSNGSNIAAATSSTY"
BASE COUNT          402 a          580 c          520 g          409 t
ORIGIN      
        1 atagcagtta tgtcctcaac gacatttagt gcgactgatt atccgaccgc agtcaaggca
       61 aactctcccg ccgggtacta ccgtctcggg gaaacctctg ggactatcgc agcctcagag
      121 gtcaactctc ccaccgttga cggcacctac acgatcacaa cgggacagtt gaactatgag
      181 tcggggatca gcgatagcac gaacgggtcg ttctactgcg gtactgggga ctttgtaatt
      241 gcccccaata actccctctt taacttcggc gacacgtttt ctctggaggc ttggtacaag
      301 ccgaccggga tcttctcggg gcagtttcgg gtgattgcca ccaagggtgg tacgtcatgg
      361 cagtttggta cgaacacatt gggccaggtt aggttgatcg gtggccctag tcagagcacg
      421 ctggtcgcca cctcaacagc ttcgctgacg gttggtgtgt ggcatcacat cgtagttacc
      481 aaatcaggat cgacgaccaa gatttacatc aacggaattg aaggaacaac cgccggcacc
      541 aatgtgacgt tcgtgaacaa tacgaacggt attgaaattg gcggttcctc gcttcaggcg
      601 atcgacgagg ttgctttcta cccgaccgcg ctgtctgcct ccgatgtcca ggcgcactac
      661 aaggctggtt cggttcctgg agctccctcc aataagaggg caccgaagct tgaaggaaca
      721 acggtcgttg gcgatcaggt tcggtgcctt accggacggt ggcttgccgc tccgacttca
      781 ttcagtttcc agtggcagtc ggcagcgaca tcgggcggga cgtacgcgaa catcaccgga
      841 gcgacgtctt cgacgtacac gctgacctcg ggtgagtcta cgaagtacgt gcgctgccag
      901 gtgaccgcct caacggtcgg aggaacgtcc tcggccgtca gtagcaacgt gcttggcccc
      961 gtaagctagc tctcgcaggg tctcatcggc gagatcggcg acgttccgca gtaacacata
     1021 agtgtcgtcg ctccagaagc attccacgac attctccggc ccgaacttgt cgaatacgcg
     1081 cctcagtcgc ggctgcttct tcctaaacac gaccacggag tctaacactt ggcgctaaca
     1141 gcaggcttca atccagcagg ggcagtaagc ggtaactcga ttctcgccac cgacgccggt
     1201 gacgcgacag cgtggaacag cgtcagcacg gtttccggct ggcccaagta ttcaaacagc
     1261 atcgccctct tcggcaagac gctttccgcg agggtcgcta ccgcaggcac cgccccgtcc
     1321 ctgagctggt cgacctcata tgcctcccca gcggaggatt acggtcgtct gtacctgtat
     1381 atcaacactc ttcctagcgc ggcgggtgcc ccgcttgtct tcggtaacgt gataaacggc
     1441 ttctcgatcg tcctcacgtc tgccgggaag atcacgatca ataagtcaac cggagccgcc
     1501 caggccacct ctgcgacgac tctttctgcc cttcagtggt acagaatcga gtaccactac
     1561 gcgacaggtg catcaacgcc aagcgtttcg atctacacga gccctgattc tgcgtcggcg
     1621 gctgagacgt tgaccgcgtc gtcgtggact gttgtcgctg ctccctcaat cctattcggc
     1681 ggtgatggta acaccaagac gtttgacctg aacgtggctc agattgccgt ccttggaacc
     1741 gcggcgatcg gcccataccc ggtcaacagt atcgccccgg ccgtgtcggg ttcagctccc
     1801 gctgggtcta ctctgacagc agcgaacgga acctggaacg gaacgttcaa ctatacgtat
     1861 cagtggactt cgaacggttc caatattgcc gccgcaacat cctcaaccta c
//
LOCUS       DATMTI010000475         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_60130, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000475 DATMTI010000000
VERSION     DATMTI010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60130"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..108)
                     /locus_tag="VN039_07690"
     tRNA            complement(33..108)
                     /locus_tag="VN039_07690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Val,seq:tac)
     gene            complement(207..1220)
                     /gene="xerD"
                     /locus_tag="VN039_07695"
     CDS             complement(207..1220)
                     /gene="xerD"
                     /locus_tag="VN039_07695"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HWV45887.1"
                     /translation="MPVSFVRMKGVRYSRHPTAMAEPTVPLLDPLLERYLSELRVEGG
                     LAINTLESYRRDLFRLQRYLRQHQLSMGDSVPPHTIRSFLASLKQEALAPSSIARLLS
                     AMRGWYRFLVRENLVEINPLRDMTTVRRPVRLPKTLASQEVTALLELPVRNRAEDQRD
                     RAMLELLYAAGLRVSELVGLNVSQIDVNRGCVRVIGKGAKERVVPIGQPACQMVVEYL
                     ERIRPTLLKGRSSRVLFISRRGRGLTRQAFWKLLLQRARRAGISKPISPHMLRHSFAT
                     HLLEGGADLRVVQSMLGHADIATTQIYTHVESSRLRHIHRRYFPRQPGRRRTRAENSM
                     KRS"
     gene            1289..>2718
                     /locus_tag="VN039_07700"
     CDS             1289..>2718
                     /locus_tag="VN039_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HWV45888.1"
                     /translation="MLAHAHNPSSTSAWPPLMVAITLALVLGIGLIPAQSGQAEAADP
                     VKILPPNPPVLNQAKHLIEKGDPESAATVLRRFLTTAPPPEHLDDTYLLLGAALYGMK
                     DYGEALHYLNQLQTEFPESDLVDRGKLMSARIHAAMGNIDLALPLLAQVRTTALDDAT
                     KREALQLTAEALAQKKDYVRAIHILLEGMAGSSDAQMAETREQIRQFITEKLDKKGLT
                     RVRDAYPRSYPGDLASLRLIDYYIGRGEDHLAERETRHFLAAFPAHPSVPRASESLEL
                     IKSRLKANQYFIAAVLPLSGHLSAFANDVLEGIQLAVERAREQPGTSSIGLIVKDHDA
                     DRQGFLDDLSTLLNDDRPLVVIGPMLSKNLPVMAEMAQKTRVPLITPAATLPNVRRLG
                     NYLFSTSLTYAMQAERIATYAAKEQGYRRFCILHPDTVYGRELARLFTQEVRRLDGEI
                     ISTEMFKEGETDFGPQIQRLKAEDLK"
BASE COUNT          616 a          815 c          716 g          571 t
ORIGIN      
        1 cttcctgttt ctgcgtacca agtagttccg aatggtaggc ggaacaggga ttgaacctgt
       61 gacctctgcc ttgtaagggc agcgctcttc caactgagct atccgcccga ttttcttagc
      121 aaccgcagcg gagcgtatca agtccatact tccttgtcaa cggtcagcgt actgtttgtc
      181 cggtttcgca atatcccgat tcctaactat gaccgcttca tcgaattttc tgcgcgtgtc
      241 cgccgccgac caggctggcg tgggaaatat cggcgatgga tgtgtcgcaa ccggctgctt
      301 tccacgtgcg tatagatttg agtcgtcgcg atatctgcat gtcccaacat ggattgaacg
      361 actcgcagat cggctccacc ttccagtaga tgggtggcaa aggagtgccg cagcatgtgc
      421 ggcgagatcg gcttggaaat accggcgcgc cgtgcccgct gcagaagcag cttccaaaat
      481 gcctgcctcg taagtcctcg tcctcgccgg ctgatgaaca gaacgcggga cgatcgccct
      541 ttgagtagag tgggtcttat gcgttctaaa tactcaacca ccatctggca tgcaggctgt
      601 ccgatcggga cgactcgctc cttagcacct ttccctatga ctcgcacaca gcctcgattc
      661 acatcgatct gcgatacgtt gagcccgaca agctccgaca cacgaagacc cgccgcatag
      721 agcaattcca gcatcgcgcg gtcgcgttga tcctcggcac ggttacgaac aggcagctcc
      781 agcaatgccg tcacttcctg cgaagccagg gtcttcggca atcggaccgg ccgccgcacc
      841 gtcgtcatat cacggagagg attgatttcc acgagatttt cacgaaccag aaagcgatac
      901 caccctcgca tcgccgagag taggcgggca atcgacgaag gcgcgagagc ctcctgttta
      961 agcgatgcaa gaaacgaccg tatcgtgtgc ggtggaacgg agtctcccat actgagctga
     1021 tgctgtctca agtatcgctg cagacgaaag aggtcgcgcc ggtaggactc gagcgtattg
     1081 atcgccagtc caccttcaac ccgcaactca ctgagatagc gttcgagaag agggtccagc
     1141 aacggtaccg ttggttcagc catggccgtt ggatgacgac tatagcgaac tcccttcatc
     1201 cgtacaaatg ataccggcat agttctcctt gacaggtcgc aacctttccg atactagtct
     1261 gcctacggcc taccaccggt cgaacgtaat gctcgcccac gctcataatc cttcttccac
     1321 ctctgcatgg ccgccgctga tggtcgccat tacccttgca ctggtgctcg gcatcggcct
     1381 cattccggct caatcgggcc aggcagaggc ggcggatccc gtaaagatcc ttcctccaaa
     1441 tccgccggtc ttgaatcagg ccaaacatct gatcgagaaa ggcgatccgg aatccgcagc
     1501 cacggtcttg cgtcgattct tgacgaccgc tccgccaccg gaacacctgg acgataccta
     1561 cctgctgctg ggcgccgcgc tctacggaat gaaggactat ggcgaggccc tccactacct
     1621 gaaccaactc cagacggagt ttcccgaatc cgacctcgtg gaccgaggca aactcatgtc
     1681 agcccgcatc catgccgcca tggggaatat cgacctggcg ctgccgttgc tggcgcaagt
     1741 acggacaacg gcactcgacg atgcaaccaa acgtgaggca ctgcagttga ctgccgaggc
     1801 attggctcaa aaaaaagact atgtccgagc catccatatc ttgctggagg gaatggccgg
     1861 cagctccgac gcccagatgg cggagacacg tgaacagatt cgtcaattca tcacggaaaa
     1921 gctggataaa aaagggttga cccgtgtgcg ggatgcctat cctcgttcat accccggcga
     1981 cctcgcgtct ctacgactga tcgattacta catcggacga ggcgaagacc acttggcaga
     2041 acgggagacc cggcattttc tcgctgcgtt tcctgctcat ccctccgtcc cgagggcaag
     2101 cgaatcgctg gaactcatca aatcgaggtt gaaagccaac caatatttta tcgcggccgt
     2161 tcttccgttg tcgggacact tatccgcctt cgccaatgac gtcctggaag gaattcagtt
     2221 ggccgtggaa cgggctcgtg aacagcctgg aacctcatcc atcgggttga tcgtaaagga
     2281 tcatgatgcc gaccggcagg gctttttaga cgatctctcg acgttgctca atgacgaccg
     2341 tccgctcgtc gtcatcgggc cgatgctgtc aaaaaatctt ccggtcatgg cggaaatggc
     2401 acaaaagacc agggtcccgt tgattacacc agcggcgaca ctccccaatg tccgccggct
     2461 gggcaactat cttttcagca cgtcgttaac gtatgccatg caagccgagc gcatcgcgac
     2521 ctatgcggca aaagaacagg gctatcgacg gttctgtatt ctgcaccctg acaccgtcta
     2581 tggacgagaa ctcgcccggc tcttcacgca ggaggtgcgc cgactagacg gtgaaatcat
     2641 ctccactgaa atgttcaagg aaggggaaac tgattttggt ccacagatcc aacggctcaa
     2701 ggctgaagac ctgaaaaa
//
LOCUS       DATMTI010000476         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_611923, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000476 DATMTI010000000
VERSION     DATMTI010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611923"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VN039_07705"
     CDS             <1..947
                     /locus_tag="VN039_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HWV45889.1"
                     /translation="SLVYLALLRIVAVGAQLVMLLFEPGYSLRQLASVASLPDSMYHP
                     LPALQLFLLPFGSDARPSLGCLTVVYWITAVAGIGGLVGWRSRFSLFIFAAGNTFLQA
                     FAYSFGDLHHREALMIITLWLLALSPSGNALSLDSHLAHRNSARPKTVQKRSMFAAWP
                     LLLVQNLFGLVYLDAALRKLYAGGVEWMNGYTLQYYLYSDASRRDSAFGLWLARQHTA
                     ACVLSWVTILWEGTFFLVLIFPRLIWIYLPLGIGLHAGMALAGIASFPQFIALYAVFI
                     PLLWKYGGRSLAIKCGVLRRMLAAETPMTNPPVQQPVT"
     gene            1006..>1543
                     /locus_tag="VN039_07710"
     CDS             1006..>1543
                     /locus_tag="VN039_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HWV45890.1"
                     /translation="MSYAHLTMVGRESAKPGIRMSMNVPGKKRISSGLLVRLAISLAI
                     VAGIVWWLGGPGKIVTIVKRIDVGLAALVILVFMLDRALMTFKWLLLLRARGIHSQFF
                     AAMKIYCASMVWGMFLPSTIGSDTIRALSIARRGINTNEVVASIVIERVFGFFATLFV
                     GLCSLLLLSLTGELEKRFI"
BASE COUNT          300 a          386 c          416 g          441 t
ORIGIN      
        1 gctctctcgt ttatctggct ctgcttagga tcgttgctgt tggggctcaa ctggtcatgt
       61 tattgtttga gcctgggtac agccttcgac aactcgccag cgtggcgtcg cttcccgatt
      121 cgatgtacca cccactaccc gcgttgcagc tgttcctttt acccttcgga tcagatgcac
      181 gcccctccct gggatgccta accgtcgttt actggataac ggcagtagcc ggcattggag
      241 ggctggtggg atggaggtcg cgtttcagtt tgttcatttt cgctgccggg aacacattcc
      301 tgcaagcttt tgcctattca ttcggcgatc tccaccaccg ggaagcgttg atgatcatta
      361 cgctgtggct gttggctctc agtccctccg gtaatgcgtt gtcactggac agtcacttgg
      421 ctcaccgaaa ttctgcgcgc ccgaaaacgg tgcagaaacg gagcatgttt gctgcgtggc
      481 cgctgctact cgttcaaaac ttgtttggtc ttgtgtactt ggatgcagct ctccgcaaac
      541 tatacgccgg cggagtcgag tggatgaatg gttacacact tcagtattac ctgtacagtg
      601 atgcttcacg aagggatagc gcgtttgggt tgtggcttgc cagacagcat acagcagcgt
      661 gcgttctttc ttgggtaacc atcctttggg aaggcacgtt cttcctagtg cttatcttcc
      721 ctcgtcttat ctggatatac ctgccacttg gaatcggctt gcatgccggc atggctttgg
      781 caggcatagc ttcgttcccc cagttcatcg cactctatgc tgtgtttatt ccgcttctat
      841 ggaaatatgg cggccgctcg ctagcaataa aatgcggggt cttgcgtcgc atgttggcgg
      901 ccgaaacacc aatgacaaac cctccggttc aacaacccgt aacataagag cattagcccc
      961 ggtctgcgtc ttggttggat gcagttggta ttatgtcgct ggtatatgtc atatgcgcac
     1021 ctgacgatgg tcggtcgcga atcggcgaaa cctgggattc gcatgtcgat gaacgttcct
     1081 ggtaaaaagc gaatatcaag tggactgctt gtgaggttgg cgatcagcct cgcgatagtc
     1141 gccggtatcg tttggtggct tggcggcccg ggaaaaattg ttacgatcgt caaacgcatc
     1201 gatgtcggtt tagccgcgct cgtcatactc gtgttcatgc tcgatcgagc cttgatgacg
     1261 ttcaaatggc tactactgct aagagccaga ggcattcatt ctcaattttt cgcagcgatg
     1321 aaaatttact gcgcctcaat ggtttgggga atgttcctgc cttcaacaat tgggtccgac
     1381 actattcgcg cactgagcat cgcaaggagg gggatcaaca cgaatgaagt tgtggcttcg
     1441 atcgttattg agcgagtttt cggatttttt gcaacgttgt tcgtgggctt gtgtagtctg
     1501 ttgttactct ccttgaccgg agaattggag aagcgattca ttc
//
LOCUS       DATMTI010000477         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_527721, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000477 DATMTI010000000
VERSION     DATMTI010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527721"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1537
                     /locus_tag="VN039_07715"
     CDS             <1..>1537
                     /locus_tag="VN039_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWV45891.1"
                     /translation="TTANGITPSSSSRDQAERRQLTVMFCDLVGSTALARRLDPEDVQ
                     AAIRRFLDACSEAVGRFNGYIAKYMGDGLLVYFGYPHAHEHDAERAVHAGLTVLEVVK
                     ALPHDSRPNEDNEIAARIGIATGPVIVGELMGQDTAKERSVFGETPNLAARLQSLAQP
                     NQLIIDPVTKRLVGHEFEFADHGAVSLKGFDTPVQAWQVLSIRLSASRFESYRASHLT
                     NFVGREQEIALLLGRWREAVDGEGQVVLLCGEAGIGKSRMTRSFCDRLAEERYQAIQL
                     QCSPYHTNTAFYPAITWLRQAAGLTSQDSAHVQLQKLDVMASESGIENQDTVSLFADL
                     LSIQGHHRDPPLTVSPETRKDMTLEALAQYLARLADRNPVLFIVEDGHWLDPTTLDLM
                     TRIIGRIRQMRVLMLIIFRPDFKPVWADYSHVTSLTLSRLPRRQSAELVAAMTGGKVL
                     PPEVQQAILAKADGVPLYIETLTENVLESGLLTEGSDSFTLKGLLKGLPIPDSLHALL
                     MERV"
BASE COUNT          335 a          430 c          453 g          319 t
ORIGIN      
        1 accaccgcga atggaatcac gccgtcctct tccagtcgag atcaagcgga acgacggcaa
       61 ttgaccgtca tgttttgtga tctggttggt tcgacggctt tagctcggcg gttggaccct
      121 gaagatgtcc aggcggctat ccgacgtttt ctcgatgcct gtagtgaagc ggtcggccga
      181 ttcaacggct atatcgccaa atatatggga gacgggctgc tggtgtattt tggatacccc
      241 catgcccatg agcacgatgc agaaagggct gtccatgcgg gactgaccgt gctggaagtg
      301 gtgaaggctc ttccccacga ttctcggccg aacgaagaca atgagatcgc agcccgaatt
      361 gggattgcaa ccggtcccgt catcgtgggc gaactcatgg ggcaggacac cgccaaggaa
      421 cggtcggtgt tcggtgaaac tccgaatctg gccgcccgcc tccaatccct tgcacagccc
      481 aatcaactga tcatcgaccc ggtgacgaaa cgtcttgtcg gacatgaatt cgaattcgcg
      541 gatcatggcg ccgtgtcgct gaaagggttt gacacccccg ttcaagcctg gcaggttctg
      601 agtatcaggc tgtcggccag ccggtttgag tcctatcgag ccagccactt aaccaatttc
      661 gtcggcagag aacaggagat tgcactcctg ctgggccgct ggcgcgaagc cgtcgacggc
      721 gaaggacagg tcgtgctcct ctgtggtgag gccggcatcg gcaaatcgag aatgacccga
      781 agtttctgtg accgcctcgc cgaggagcgg taccaggcga ttcaattgca atgttcaccg
      841 taccatacca atacggcgtt ctatccggcg atcacttggc tacggcaggc cgcgggactg
      901 accagtcagg atagtgccca cgtgcaactg cagaagctcg acgtcatggc gagcgaaagc
      961 ggcatcgaga accaggacac ggtgtcctta tttgcggatc tgctttcgat ccaaggacat
     1021 caccgggacc cgccgctcac tgtgtcgccc gaaacacgca aggacatgac gctggaagcc
     1081 ctcgcgcagt atctagcacg actggccgat cgcaaccccg tgttgttcat tgtggaagat
     1141 ggccattggc tcgatccaac aacgctggac ctcatgacgc gaatcatcgg ccgcattagg
     1201 cagatgcgcg tgttgatgct gatcattttt cgaccagact tcaagccggt gtgggcggac
     1261 tacagtcacg taacatccct gacgctcagt cggcttccac gtcgacagag cgccgaactc
     1321 gtggcggcga tgacaggagg aaaagtgctt ccgcctgagg tgcagcaggc gattctggcg
     1381 aaggccgatg gcgtccccct ctatatcgaa acgttgacgg aaaatgtgct tgagtccgga
     1441 ctgctgactg aggggagcga ttcatttacc ttgaagggcc tattaaaggg attgcccatt
     1501 ccggatagtc tgcacgcgtt actcatggaa cgggtag
//
LOCUS       DATMTI010000478         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_400608, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000478 DATMTI010000000
VERSION     DATMTI010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400608"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VN039_07720"
     CDS             <1..717
                     /locus_tag="VN039_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWV45892.1"
                     /translation="LDNTAGSLDFAWDDSFFVMDALDQSKSRIEAAVPGATIEIVQNG
                     SPSQQHSLLIDNDHAVPVARFLRVDPALCLDFCSNVTGVDWLDRVVKKTTKVKKLVDG
                     QEKEIVETSEEKIPGYLEAVYHLYSMTHKHGPVIVRMRTNDRADGNHLPSLTPIWRSA
                     EFQEREIFDLYGIRFEGHPDLRRILMWDEFEDHPMRKDYREPDDYEWEPTPHDEVLER
                     AKQHYPARPTEEDLNHPDLS"
     gene            830..>1528
                     /locus_tag="VN039_07725"
     CDS             830..>1528
                     /locus_tag="VN039_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWV45893.1"
                     /translation="MGSRLEGELLEVSMGPQHPSTHGVFRMNVALDGEIVRKLKPVFG
                     YLHRNHEKIGENTSYLGSMPYTDRLDYFCSMTNNWAYALSVEKLAGIEVPERAEYLRI
                     ILAELTRLQNHASLLGFLLSDMGAWGTPLMYAFREREKILDLFESLAGSRMMCDYMRF
                     GGCRVDANDEWLARAQQIVDRFPKFLDEFEELILGNEIVIGRTQNVGNLSAELAINAG
                     ITGPMLRACGVNYDL"
BASE COUNT          350 a          400 c          437 g          341 t
ORIGIN      
        1 ctagacaata ctgcgggatc cctcgacttc gcttgggatg acagcttttt cgtcatggat
       61 gctctcgacc aaagcaaatc ccgcatcgaa gctgctgtac ctggcgccac gatcgagatt
      121 gtgcagaacg gcagcccgtc ccagcagcat tcgttgttaa tcgataacga ccatgcggtc
      181 ccggtggcga gatttttgcg ggtcgatcct gcgctgtgcc tggatttttg ctccaatgtc
      241 actggcgttg attggctcga tcgcgtcgtc aagaaaacaa cgaaggtaaa gaaactcgtc
      301 gacggtcagg agaaagagat cgttgaaacc agcgaagaaa aaattcccgg atatctcgag
      361 gcggtttatc acctttactc gatgactcat aaacacggtc ccgtgatcgt tcgtatgcgc
      421 accaacgatc gcgctgacgg taatcacttg ccgtcgctca ccccgatctg gcgtagtgcg
      481 gagtttcagg aacgcgaaat tttcgatctt tacggcatcc ggttcgaagg tcatcccgat
      541 ttgcggcgca ttttgatgtg ggacgagttt gaggatcatc cgatgcgaaa ggattaccgt
      601 gagccggatg attatgaatg ggaaccgacg ccgcacgatg aagtgttgga aagggcgaag
      661 cagcattatc ctgcgaggcc gaccgaggag gacctaaacc atcctgatct gtcatgaagt
      721 ctaggaatga atccagagat ccttcatcgt ctgcgcgact caggatggca atctgaagat
      781 gagcggagtg gttgaaacaa tcgaagcaat ggagcgaccg ccgttgacca tgggttcgcg
      841 gctggaaggc gaactgctcg aagtctcgat gggaccgcag catccatcca cgcacggcgt
      901 gtttcgaatg aatgtggcgc tggatggcga gatcgttcgt aagctcaaac cggtcttcgg
      961 ttacttgcat cggaaccatg aaaaaattgg cgagaacacc agttacctcg gttcgatgcc
     1021 gtacaccgac cggctcgatt acttttgctc gatgactaac aactgggcgt acgccctcag
     1081 tgttgagaag ctcgccggga tcgaagttcc ggaacgcgcc gaatatctgc gcatcatact
     1141 ggccgagctt acgcggttgc aaaatcacgc ttcgctactc ggctttctgc tcagcgacat
     1201 gggcgcttgg ggcacaccac tgatgtacgc ctttcgggag cgcgaaaaaa ttctcgacct
     1261 gttcgagtcg ttagccggct cgcgcatgat gtgcgattac atgcgcttcg gcgggtgccg
     1321 cgtggacgcg aacgatgagt ggctcgcccg ggcgcaacag attgtggatc ggttcccgaa
     1381 gttcctcgac gaattcgagg aactcatcct cggtaatgag atcgtcatcg gccggactca
     1441 aaacgtcggg aacctctctg ccgaactggc gatcaacgcc ggcatcacag gtccgatgtt
     1501 gcgcgcttgc ggtgtgaatt acgacctg
//
LOCUS       DATMTI010000479         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_485416, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000479 DATMTI010000000
VERSION     DATMTI010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485416"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /gene="dnaJ"
                     /locus_tag="VN039_07730"
     CDS             <1..999
                     /gene="dnaJ"
                     /locus_tag="VN039_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWV45894.1"
                     /translation="KKSAEEKFKEINEAYETLSDQERRKRYDMFGHAGAQQGGSGFDG
                     FDFGRGGFGDVFNDIFEDFFGQARGGGATRAERGNDLQYNLEITFEEAVYGKEAKLKI
                     PRWEICVDCKGTGAKSAAAIKPCASCKGTGQLRFQQGFFSVSRPCGQCEGTGHFVTEP
                     CSTCQGRQRVYKERTIAVHIPAGIETGMRLRLSNEGEHGPNGGPPGDLYVAVTVRPHQ
                     VFHRKGLDIACDVPVNLVTAVLGGKVEVPTLTGATVIKVPPGTQHDKVLRIKGLGIPS
                     LKGGSTGDQVYTIKVQIPTKLTAKQKELLMEYAKESGMSMEANGDGFFDKMKTFFE"
     gene            1036..1776
                     /locus_tag="VN039_07735"
     CDS             1036..1776
                     /locus_tag="VN039_07735"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HWV45895.1"
                     /translation="MPVFFVPSQCITPPTISITGDLLSHLRDSLRVNIDASLWFGNGQ
                     STRYHAEITDVSKRAAIGRILETVQEPLRRTPRLILGQSLLKGEKMDWVIQKATELGV
                     AELVPIESRHSVVQLKADRVDHQLARWQRIALEAAQQSEQWRVPTIAPPQHLAALLTA
                     CATGTITLMLAERREGKSLQSVELPQDATSSLLVLIGPEGGWSQEEMQMAEQAGVQSI
                     TLGQQILRAETAAIATISILQSRLGGLG"
     gene            1912..>2123
                     /locus_tag="VN039_07740"
     CDS             1912..>2123
                     /locus_tag="VN039_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45896.1"
                     /translation="MAVNEYNFRWMYLQNFRTAVDYFAKILSEKVIPGFEGLPEEADA
                     LAQEVYKKLGMSFDPDHDDPADSAEAA"
BASE COUNT          527 a          588 c          587 g          421 t
ORIGIN      
        1 aagaaatccg ccgaagagaa gtttaaggaa atcaacgaag cctatgaaac actgagcgat
       61 caggaaagac ggaaacgcta cgacatgttc ggccatgccg gggcacagca gggcgggtca
      121 ggctttgacg ggttcgattt cggccgtggc ggattcggag acgtgtttaa cgacatcttc
      181 gaggattttt tcggccaagc gcgcggaggc ggggcaactc gggctgaacg gggcaacgat
      241 ctccaataca accttgaaat cacgtttgag gaagccgtct acgggaaaga agccaaactc
      301 aagatcccgc gctgggaaat ctgtgtcgac tgcaaaggaa ccggggcgaa gtcggcggca
      361 gccatcaagc catgtgccag ctgcaaaggt acggggcaac ttcggtttca gcagggcttc
      421 ttcagtgtca gccggccctg tggtcaatgt gaaggcaccg gtcactttgt cacggaaccc
      481 tgctctacct gccagggacg tcaacgggtt tataaagagc gcaccatcgc cgtccatatt
      541 ccggcgggta ttgagaccgg catgcgactt cgtctctcca atgaaggaga gcatggtccc
      601 aatggcggcc ctcccggcga cctctacgta gctgttaccg tcagacccca tcaagtcttt
      661 catcgcaagg gcctggatat cgcctgtgat gtgccggtca acctcgtcac agccgtgctc
      721 ggtggaaaag tggaagtccc aaccctcaca ggagcaaccg tcattaaggt gccgccgggc
      781 acacaacatg acaaggtcct tcggattaaa gggttaggga tccccagcct gaagggaggc
      841 tcgaccggtg atcaggtcta tacgatcaag gtccaaattc ccaccaaatt gacggccaag
      901 caaaaagaac tgctgatgga gtacgcgaaa gagagcggca tgtccatgga agccaacggc
      961 gacggcttct tcgataagat gaaaaccttc ttcgaataat cttccctacc ctcgttacgt
     1021 cctgcagagt cactcatgcc cgtgtttttc gttccctctc agtgcattac accaccgacc
     1081 atttccatca ctggcgatct gctgagtcac ctccgagaca gtttgcgcgt gaacatcgat
     1141 gcgtccctat ggttcggtaa cggacagagc accaggtacc atgccgaaat caccgacgtc
     1201 tccaaacgag cggccatcgg acggattctc gaaacggtcc aagaaccgct tcgtcgcacg
     1261 cctcggctga tcctcggcca atccttactc aagggcgaaa agatggattg ggtgattcag
     1321 aaagccaccg aactgggcgt ggctgaactc gtgccgattg aaagccgaca cagtgtggtg
     1381 caactcaaag ccgaccgtgt ggaccaccaa cttgcccgtt ggcaacgcat cgcgttagaa
     1441 gccgcccagc agtccgaaca atggcgcgtg ccgacgatcg ccccgccaca acacctcgca
     1501 gcacttttga ccgcctgtgc aaccggcaca atcacactca tgcttgccga gcggcgggag
     1561 ggaaaaagtt tgcaatcagt cgaactccca caagatgcga ccagctcctt gctggtcttg
     1621 atcggaccgg aaggaggctg gagccaagaa gaaatgcaga tggccgagca ggcaggagtc
     1681 caatctatca cccttggtca gcaaatcttg agagctgaaa cagccgccat cgcgaccatc
     1741 agcattctcc aatcgcgcct cggcgggctg ggatgaccga accgatgcta gtggagggac
     1801 cgctgttgct tttatctcgc cgtggcggct agagctagtg ggtcagaaac gcattcccgt
     1861 gaatgcatga aggccagacc ttcgttttca acgtgtaaat gggctatcta tatggccgtt
     1921 aatgagtaca acttccgctg gatgtaccta cagaactttc gaacagcagt ggattatttc
     1981 gccaagattt tgagcgaaaa agtcatccct ggattcgagg gacttccaga agaggctgat
     2041 gcactagcgc aagaagtcta caagaagttg gggatgtcgt ttgatccaga ccatgatgat
     2101 ccggctgact cagcggaagc tgc
//
LOCUS       DATMTI010000480         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_272396, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000480 DATMTI010000000
VERSION     DATMTI010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272396"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1666
                     /locus_tag="VN039_07745"
     rRNA            126..1666
                     /locus_tag="VN039_07745"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1819..1894
                     /locus_tag="VN039_07750"
     tRNA            1819..1894
                     /locus_tag="VN039_07750"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1852..1854,aa:Ala,seq:tgc)
BASE COUNT          455 a          448 c          642 g          401 t
ORIGIN      
        1 agaagatgag agccttgcga gctcgggatt caggtctcga gtaagcagac gtaaccaaaa
       61 gataagaacc gagccggtag cggatgcccg agagggtatt tgcgaaggat cgttctgtag
      121 ggattaaaca tgagagtttg atcctggctc agaatgaacg ctggcggcgt gcctaacaca
      181 tgcaagtcgg atgtgcccgc aagggtgcat ggcagacggg tgagtaacgc gtggggaatc
      241 taccttttag tggggaataa cgctccgaaa ggagtggtaa taccgcatgt gaccatcggc
      301 tgggatgctg gtggtgaaac cctgggacct tcgggccagg ggctagaaga ggatcctgcg
      361 tctgattagc tagttggtgg ggtaacggcc taccaaggcg acgatcagta gccggcctga
      421 gagggtgatc ggccacactg gaactgagac acggtccaga ctcctacggg aggcagcagt
      481 ggggaatttt gcgcaatgga cgaaagtctg acgcagcaac gccgcgtggg tgatgaaggt
      541 cttcggattg taaaaccctg tcgccaggga cgaagggtgg ggcgttaata gcgctttact
      601 ttgacggtac ctggaaagga agccccggct aactctgtgc cagcagccgc ggtaatacag
      661 agggggcaag cgttattcgg aattattggg cgtaaagggc gcgtaggcgg ttttttaagt
      721 cggatgtgca atccctgggc tcaacccagg aactgcatcc gagactggaa ggctagagta
      781 ctggagaggg tggtggaatt cctcgtgtag cggtgaaatg cgtagagatg aggaggaaca
      841 ccagtggcga aggcggccac ctggacagta actgacgctg aggcgcgaaa gcgtgggtag
      901 caaacaggat tagataccct ggtagtccac gctgtaaacg atgaacactt ggtgtgccgg
      961 gagttgaccc ccggcgtgcc ggagctaacg cgttaagtgt tccgcctggg gagtacggtc
     1021 gcaaggctga aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt
     1081 aattcgacgc aacgcgaaga accttacctg ggcttgaact gcagtggacc ggtgcagaga
     1141 tgtgcctttt cgcaagaact gctgcagagg tgctgcatgg ctgtcgtcag ctcgtgtcgt
     1201 gagatgttgg gttaagtccc gcaacgagcg caacccttac ctctagttgc cagcggttcg
     1261 gccgggaact ctagagggac tgcccgggtt aaccgggagg aaggtgggga tgatgtcaag
     1321 tcctcatggc ccttatgtcc agggctacac acgtgctaca atgggcgata caaaccgttg
     1381 caaagtcgcg agatggagcc aatcggagaa agtcgccctc agttcggatt gcagtctgca
     1441 actcgactgc atgaaggtgg aatcgctagt aatcgtgtat cagaatgata cggtgaatac
     1501 gttcccgggc cttgtacaca ccgcccgtca catcacgaaa gccgggagca cttgaagagg
     1561 ctgtggtaac ccgcaagggg gctaggtctc gatggtgaac ttggtgattg gggtgaagtc
     1621 gtaacaaggt agctgtagga gaacctgtgg ctggatcacc tcctttctaa ggagaagcgt
     1681 gacccttcgg ggtgagcgct acggtcaacc tctcatttgc tcaagcctag gcttgagcta
     1741 tccatccggt tttcaaagac gcctgaagcg tctttgccga tatgtctttg gaagaagaga
     1801 agtagggttc cgagtcatgg gcccgtagct cagctgggag agcgcctggt ttgcatccag
     1861 gaggtcgtcg gttcgaaccc ggtcgggtcc accaccacgg tcgatggatg cttcaccctg
     1921 ttcattcagg ccaggttgcg aatgcg
//
LOCUS       DATMTI010000481         7688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_231960, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000481 DATMTI010000000
VERSION     DATMTI010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231960"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2410
                     /gene="ligD"
                     /locus_tag="VN039_07755"
     CDS             <1..2410
                     /gene="ligD"
                     /locus_tag="VN039_07755"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HWV45897.1"
                     /translation="EGTIPSRQYGAGTVMVWDRGQWIPDGDPQAAYEKGHLKFRLAGQ
                     KLQGRWTLVRIGGARNREGNNWLLIKEDDEAARKAMAIDSTPTLSNSAKSGMSMDEIS
                     TGRAAEWHSDRATDRHTGAVRTEVDRTIHPRGDRQSRTRVKRGTERTSCPEWMEPQLA
                     TLVDEVPKDEGWLYELKYDGYRMLCRISNGVAKLFSRSGREWTAKLSEHAKIAADLRV
                     ANAWLDGEIVALAADGTMSFQALQNAFDRVKTGRLVYFVFDLLFLDGNDLRGMPLQER
                     KRRLSALLAGQPDDGPLRYSDHIREEGRTVFEAACRQGLEGLIAKQADSKYLPGRNRN
                     WIKLKCRRRQEFVIGGFTDPSGSRRGFGALLLGVYEGKGNLTYVGRVGTGFSGERLSR
                     LHRTLRSLQRPLPAFVKPPTGADAKGVHWVKPQMVAEVRFAEWTQEGLLRQASFVGIR
                     HDKPAAVIVRESPTHVPASQLTSDRRDEGAVRNPKQSSSRANRVRSSNQGQKRSGEKT
                     VAGVRISNPDRVLFPHDGITKLQLAQFYERIAGWILPQLRGRPLTLMRCPDGEGTGCF
                     YQKHVTDQVPKAIDRVEVEEADGSACYMMANTPAALVAFVQLGVLEFHTWGATQDRLD
                     RPDRMILDLDPAPDVPWPQVVEAALLIRTLLDELSLASFIKTTGGKGLHVVVPLERRH
                     SWDEVKQFAKSLAGHMVRIVPGHFTDNMSKRARKGKVYLDYLRNGRGATAVAAYSTRA
                     KPRAPISVPLTWDECSPELRSDRFTLHNIEERLSRLAKDPWAEYAKVRQHLTESMKRR
                     FADR"
     gene            2535..6209
                     /locus_tag="VN039_07760"
     CDS             2535..6209
                     /locus_tag="VN039_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV45898.1"
                     /translation="MDHPPLSDLLKEGPKGPPSDPAFLSGGGEMGERMRAFDWSETAL
                     GAIENWSPALRMMTSFLLSNRFPLLLWWGPQYVSIYNDAYIPVLGTKHPWALGQSVSE
                     CWKEIWHILQPLIDTPFNGGPATWNDDITLEINRHGFVEETHFTIAYSPVPDETVPSG
                     IGGVLATVHEITQKVIGERRIALLRDLGARLAEGRTAEDVCRIAAKIIGQHPKDIPFA
                     LFYLIDKDEKHARLVGSCGAIGKEESPSIITLEKDSAESIWPLAAVLREETPQLVGDL
                     SARLSVIPSGPWSDPPQNAIVVPVASCMAHPYTGMLVLGVSARLRFDNVYRGFVELLA
                     AQVAMAIVNARAYEEERKRAEALAELDRAKTGTAHLPEDRIGTPGRHTSPARKVSPFV
                     EEALRWLPSEDRTEDSGLRTEVIETALPLLGPQHSVLSTEVARPRVLVADDNADMREY
                     VLRLLSSQGYEVEAVTDGQAALDRAQANPPDLVLTDVMMPRLDGFGLLHALRNDPATK
                     TIPVILLSARAGEAFRVEGLEHGADDYLIKPFSARELLARVETHLRMASMRRDAEAAL
                     RESEARSREMIDALPAAIYTTDAHGRLTHFNPAAVEFSGRVPELGTDEWCVSWKLYYP
                     DGTPMPHDQCPMAIALKEGRTVRGAEAIAERPDGTRVWFMPYPTPLRDAEGNIAGGIN
                     MLVDITERKRAEEAKVRLSAIVESSDDAIISKDLQGIITSWNKGAEKVFGYTADEAIG
                     KPITMLFPPDCTDEEPRILKRIARGETLEHYETVRRRKDGTDIHISLTVSPIRDEAGT
                     IKGASKIARDITRRKRVEEALRRLNDQLEQRVAERTYELAQSEDHLRTLATELNLVEQ
                     RERKRLATELHDHLQQMLVLGRLTIGQGKRVAAGVPAYEQVLKKVDDIFSDALTYSRT
                     LVSDLSPTVLRDHGLTAALQWLGTYMQKHNQTVIVTVPDDGDLKLPEDQVILLFQSVR
                     ELLINSSKHAGTGEATLRMEQHDGLLRIEVRDEGAGFDLAAAVAGTPSGGISSKFGLL
                     SIRERMRALEGSFSIQSAPGEGTTATLTLPLRSSAEVRDATNKERHVNGRRRDGEEAV
                     LLPATQSSALQQPAKIRVLLVDDHPTMRQGLRSIVTAYDHLEVVGEASNGAEAVELAQ
                     GLDPDVVVMDINMPKMDGIEATQQIKANQPTTVIIGLSVNQSADTEKKMKAAGVSTYL
                     TKESAVDVLCHAIEQAVSYK"
     gene            complement(6291..7409)
                     /locus_tag="VN039_07765"
     CDS             complement(6291..7409)
                     /locus_tag="VN039_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWV45899.1"
                     /translation="MKIAQVAPLWESIPPHRYGGTERIVSCLTEELVRLGHDVTLFAA
                     GNSVTSATLRAICPSSLRSTPQMFPDASFSLLLERAFGTYAGHFDLIHSHIDLLGFPL
                     ARRCRTPVLTTVHTRLDLPELIPVFEQFPELPLVSISDAQRCSMPGANWQSTIHHGLS
                     RNQYSLHRNLGTYLAFLGRISPGRGVEQAIELAKRTEMPIRIAARVDATDRAYFEHIA
                     QLLEHPLVEYLGEITDEEKNDFLGDAYALIFPVDRPEPFSLVLIEALACGTPVLAYRR
                     GAVPEIVDHGSTGYLCDTLEEMTHTVAWVPNLDRRRCREAFEERFTVERMVQDYLLVY
                     QRSVHKENDRSADQGSYEVMRQFPPSTTMEDARVVEAA"
BASE COUNT         1746 a         2102 c         2320 g         1520 t
ORIGIN      
        1 cgaaggcacc attccctctc gacagtatgg ggccggcacc gtcatggtct gggatcgagg
       61 acagtggatt cccgacggag acccacaggc ggcctatgag aaaggacacc ttaaatttag
      121 actggcggga caaaaactgc agggacgctg gaccttggtg cggattggcg gagcgcgcaa
      181 tcgggaagga aacaactggc tgctcatcaa ggaagatgat gaagccgccc gtaaggcaat
      241 ggccatcgat tcaacgccca cgttgtcaaa cagcgcgaaa agtggaatga gcatggacga
      301 aatctccact ggccgggcgg cggaatggca ttcggatcga gcgacagacc ggcataccgg
      361 agcggttcgc acagaggtcg atcgaacgat ccatccgagg ggcgatcgac agtctcggac
      421 cagagtcaag cgaggaactg aacggacatc ctgcccggaa tggatggagc cgcaactggc
      481 cacactggtg gatgaagtcc ccaaggacga aggctggctg tatgagctga agtacgacgg
      541 ctaccgcatg ctctgtcgga tcagtaacgg cgtcgcgaaa ctattttcaa gaagcggccg
      601 ggaatggacg gcgaagttgt cggaacacgc aaagatcgca gccgatttgc gcgtcgccaa
      661 tgcctggctc gacggcgaaa tcgtggcgct tgctgcggat ggaacgatga gtttccaagc
      721 gctgcaaaat gcattcgatc gtgtgaagac cggccgtctt gtctatttcg tgttcgacct
      781 cctctttctc gatgggaacg atcttcgcgg gatgccgctg caggaacgta agcggaggct
      841 gtcggcgctg ttggccgggc aaccggacga cggtcccttg cgttacagcg accatattcg
      901 agaggaaggc cgcaccgtct tcgaagcagc ctgccgtcag ggtctggaag ggctgatcgc
      961 caagcaagcg gacagtaagt atcttccggg gcgcaaccgc aattggatca agctgaaatg
     1021 ccggcgtcgt caggaatttg tgatcggcgg attcaccgac ccgtccggtt cgcggcgtgg
     1081 cttcggcgca ttactgcttg gtgtgtacga gggaaagggg aatctgacct atgtggggcg
     1141 agtcggaacc ggattttccg gtgaacggct ctctcgattg catcgtacct tgcgatcgct
     1201 gcaacgtccg ctgccggcat tcgtcaagcc cccgaccggc gcggatgcca agggagtaca
     1261 ctgggtgaaa ccgcaaatgg tggccgaagt gagatttgca gaatggacgc aggaagggct
     1321 tctccgtcaa gcttcatttg tgggcatacg tcatgacaag ccggccgcag tcatcgtgcg
     1381 tgaatcaccg acacatgtgc cggcgagtca gcttacgtcc gaccggcgag acgaaggagc
     1441 ggtccgtaat ccgaagcagt cttcctcccg tgccaatcga gtccggtctt ccaaccaagg
     1501 tcaaaagcgc tcgggggaaa agacagtggc aggtgtcagg atttcgaatc ccgatcgcgt
     1561 cttgttccct cacgacggca tcaccaaact gcaactcgcg caattctacg agcgtatagc
     1621 gggctggatc ctgccgcagc tgcgagggcg tccattgacg ctgatgcgtt gtccggatgg
     1681 ggagggcacc gggtgttttt atcagaaaca cgtgacggat caggttccta aggcgattga
     1741 tcgcgttgaa gtcgaggaag cggacggatc cgcttgctac atgatggcca atacgcccgc
     1801 tgccttggtc gcgtttgtgc agctcggtgt tctggagttc catacgtggg gtgccacgca
     1861 agatcggctg gatcggccgg atcgtatgat cctcgatcta gatcccgcgc cggatgtgcc
     1921 gtggcctcaa gtggttgagg cggcactgct cataagaaca ttgcttgatg aattgagcct
     1981 ggcatctttt atcaagacca ccggcggaaa agggttgcac gtcgtggttc cattggaacg
     2041 ccggcactcg tgggatgagg tcaaacaatt cgccaagtcc ttggccggac atatggtacg
     2101 aatcgttcca ggacacttta ccgataacat gtcgaagcgc gcgcgcaagg gaaaggtgta
     2161 cctggattat ttgagaaacg ggcgaggcgc gactgcggtg gccgcctatt cgacgcgcgc
     2221 caaaccgcga gctcccattt cagttccgct gacttgggat gagtgttctc ctgagctacg
     2281 ttcggaccgg tttacactgc acaacatcga agaacggttg tcacgacttg ccaaggaccc
     2341 ttgggccgag tatgccaagg tacgtcaaca tttgacggag agtatgaaga gacgtttcgc
     2401 agaccgttga aaaagatctc gcgcccatgg ccgatctagg atttccccta gtttgtactg
     2461 gcgacgatat gttttactaa taccagcggt ccaatccatc attgtcattt atctaccaag
     2521 tagacgaagg ttccgtggat cacccgccat tatccgatct gctaaaggaa ggacccaaag
     2581 ggccgccatc cgatccggcg ttcctctcgg gcggcggcga gatgggcgag cggatgcgcg
     2641 ccttcgactg gtccgaaacg gcactggggg cgatagaaaa ctggtccccc gcactccgaa
     2701 tgatgacgag tttcctgctt tccaaccgct ttccattgct cttgtggtgg ggacctcagt
     2761 atgtatcgat ctacaacgat gcctatattc cagtgcttgg gacgaaacat ccatgggcac
     2821 tgggtcagtc tgtcagcgag tgttggaaag aaatctggca catccttcag ccgctgatcg
     2881 atacgccgtt caatggcggt ccggcgactt ggaacgacga catcactctt gaaatcaatc
     2941 gccacggctt tgtcgaagag acacacttta ccattgccta cagcccggtg ccggatgaaa
     3001 ccgtcccaag cggcattggg ggcgtactcg ccaccgtgca cgaaattact caaaaggtta
     3061 taggagaacg ccgcatcgcg ttgctcaggg atctcggtgc ccgtttggcc gaaggtagaa
     3121 ccgcggagga cgtctgtcga atcgcagcca aaatcattgg acagcatccg aaagatatcc
     3181 ctttcgcact gttttatttg atcgacaagg atgagaaaca tgcgcgcttg gtaggatcct
     3241 gcggcgccat aggaaaagaa gagagtcctt cgataatcac acttgagaag gattcagctg
     3301 aatctatctg gccgttggct gccgttttac gcgaagaaac tccgcaactc gtcggcgatt
     3361 tatcggctcg gttatcggtg attccttcgg gaccgtggtc cgaccccccg caaaatgcga
     3421 ttgtcgttcc ggtcgcttcg tgtatggcac acccgtatac cgggatgctt gtgctcggtg
     3481 tcagtgcccg ccttcgattc gataatgtct atcgcgggtt tgtcgaacta ctggccgcac
     3541 aggtggccat ggctatcgtt aatgctcgcg catatgagga agaacgcaag cgggccgaag
     3601 cactggctga actggaccgc gccaagaccg gaaccgcgca ccttccggaa gatcgcattg
     3661 gcaccccagg ccgacatacc tccccggcga ggaaagtgag cccgttcgtc gaagaagcgt
     3721 tgcgctggct gccatccgaa gacaggactg aggactcagg actgaggact gaggtcatag
     3781 aaactgcatt gcctttactc ggtcctcagc actcagtcct cagcactgag gtcgcgcgtc
     3841 cgcgcgtgct ggtcgccgac gacaatgccg acatgcggga gtatgtgctg cggctgcttt
     3901 ccagccaggg ctatgaggtc gaagcagtca cggatggcca agccgcgctc gaccgcgctc
     3961 aagcgaatcc acccgacctc gtgctgaccg acgtcatgat gccacggctg gacggtttcg
     4021 gattgctcca cgcgttgcgc aatgatcctg cgaccaagac cattcccgtg attctgctgt
     4081 cggcaagagc cggggaagca ttccgcgtgg agggtttaga acacggcgcc gacgattatc
     4141 tcatcaagcc cttcagcgcc cgcgaattgc tggctcgcgt cgagacgcac ctgaggatgg
     4201 ccagcatgcg acgcgacgcc gaggctgcgc tgcgcgagag cgaagctcgc tctcgtgaga
     4261 tgatcgatgc gttgccggcg gccatctaca ccaccgacgc gcacggtcgc ctgacgcatt
     4321 tcaacccggc cgccgtggag ttctccggcc gcgtgccgga gctaggcacg gatgagtggt
     4381 gcgtgagttg gaagctctac tatcccgacg gtacgccgat gccgcacgac caatgtccga
     4441 tggccatcgc actgaaagag gggcgcacgg tccgcggggc tgaagcgatc gccgagcgcc
     4501 ccgacggcac gcgcgtctgg ttcatgccct acccgacacc gttacgcgat gccgagggca
     4561 acatcgcggg cggcatcaat atgctggtag acatcaccga gcggaagcgg gccgaggaag
     4621 cgaaggtcag gctcagtgcc atcgtcgagt cctcggacga tgccatcatc agcaaggatt
     4681 tgcagggcat tatcacgagt tggaacaaag gcgcagaaaa ggtgttcggg tatacggcgg
     4741 acgaagccat cggcaagccg atcacgatgc tgtttcctcc ggattgcacc gacgaagaac
     4801 cacggattct caaacgtatc gcgcgcggtg aaacgttgga acattacgaa accgtgcgcc
     4861 ggcgcaaaga cggaaccgac atccatattt cgcttaccgt atcaccgatt cgggacgagg
     4921 ccggaacgat caagggggca tcaaaaatcg cgcgcgacat caccaggcgt aagcgggtgg
     4981 aagaggctct gcgccgattg aacgaccagt tggaacaacg cgtcgccgaa cgcacgtatg
     5041 aacttgctca gtcggaagat catcttcgca ccttggccac ggaactcaac ctggtcgagc
     5101 agcgggagcg gaaacggttg gcgaccgagc tgcacgacca tctgcaacag atgctggtgc
     5161 tgggcaggct cacgatcgga cagggaaagc gcgtagcggc cggcgtaccg gcctatgagc
     5221 aagttctcaa gaaagtggat gacatcttct ccgacgcgtt gacctatagc cgtaccctcg
     5281 tctccgacct cagccccacc gtcctgcggg atcatggcct gacggcggcg ctgcaatggc
     5341 tcggcaccta catgcagaaa cacaatcaga ccgtcatcgt gactgtgccg gacgacggcg
     5401 atctgaaatt gccggaagat caagtcatct tgttgttcca atccgtgcgg gaacttctga
     5461 tcaattcctc caagcatgcg ggaaccggtg aagccactct ccggatggaa cagcacgacg
     5521 gcctgctgag aatcgaagtt cgggatgagg gcgcgggctt tgatcttgct gctgctgttg
     5581 ctgggacacc cagcggaggc atttcctcga agttcggcct cctcagtatc cgagagcgta
     5641 tgcgggcgct ggaggggagt ttcagcatac aatcggcgcc tggagagggg accacggcga
     5701 cactgacgtt gccgctccga agcagtgctg aggtccgcga cgcgacgaat aaggagcgtc
     5761 acgttaacgg acgccgccga gatggtgagg aggcagtgct ccttcccgcg actcagtcct
     5821 cagcactcca gcagcctgcg aaaatccgcg tgctgctggt ggacgatcac cccacgatgc
     5881 ggcaaggctt gcggagcatc gtgacggcgt atgaccatct cgaagtcgtc ggcgaggcga
     5941 gcaatggagc ggaagcggtc gaactggcgc agggcctcga tcctgatgtg gtggtgatgg
     6001 acatcaatat gccgaagatg gatgggatcg aagcgacgca gcagatcaaa gcgaatcagc
     6061 cgaccaccgt catcatcggc ctgtcggtga atcaatcagc cgatacggag aaaaaaatga
     6121 aagcggcggg cgtgtccacc tatttgacca aagagagcgc ggttgacgtg ttgtgccacg
     6181 ccattgaaca ggccgtgtca tacaaataac cccacggttg ctcgtccagt ccattaacca
     6241 tagccggtcg gattcttagt cggattctta ggatggtcga ttgttctcta ttacgctgct
     6301 tccacgactc gtgcatcctc catggtcgtt gaagggggga actgccgcat gacttcatag
     6361 gacccttgat cagccgaccg gtcgttctct ttgtgcactg atcgctgata caccaagaga
     6421 tagtcctgca ccatgcgctc gacggtaaac cgttcctcga atgcttcccg gcatcggcgt
     6481 cgatcgagat tgggtaccca tgccactgta tgggtcatct cctccaaggt gtcgcagaga
     6541 tatcccgtag atccgtgatc cacgatttcc gggacggctc cacgccgata ggccaacacg
     6601 ggcgtaccac acgcgagggc ttcgatcaag accaagctga agggttccgg ccgatcgacc
     6661 gggaagatca acgcatacgc gtcgccgaga aaatcgttct tctcctcatc ggtaatttcg
     6721 ccaaggtatt cgactaatgg gtgttccaat agttgagcga tatgctcgaa atacgcgcga
     6781 tccgtcgcat cgaccctcgc ggcaatccgg atcggcatct cagttcgctt ggccagctcg
     6841 atggcctgct ccacccctct gccgggcgag atccggccca agaaggcgag gtatgtgccg
     6901 agatttcggt gcaaggaata ctgatttcga gacaatccat gatggatggt gctctgccaa
     6961 ttggccccag gcatcgaaca tcgctgtgcg tccgatatcg agaccaacgg tagctcgggg
     7021 aactgttcaa acaccgggat cagttcaggc agatcgagcc gtgtatggac ggtggtaagc
     7081 accggcgtgc ggcatcggcg ggcaaggggg aagcccaaga gatcaatatg tgaatggatc
     7141 aggtcgaaat gtcccgcata cgttccgaat gcacgttcaa ggagaagtga aaacgaagcg
     7201 tctgggaaca tttgcggggt tgaacgcaag gacgatgggc agattgcgcg aagagttgcc
     7261 gatgtaaccg aattaccggc ggcgaagagg gtgacgtcat gaccaaggcg aacgagttct
     7321 tcagttaaac atgaaacgat acgttcagtg cctccatagc ggtgcggagg aatgctttcc
     7381 cacaacggcg caacttgagc gatcttcatc ttgatccttc ctgatgatat agataatgac
     7441 acccccagcc gtcacaccga tcgttcgaga cgggaacggt ttgcacgatc cggtggaaag
     7501 agtatgcgaa tggtatatgt aataaaacga gggagatccc tagatttgag gagttcagtg
     7561 ctcggacgag agaataataa tggaatgaat gcactaatcc tgtttccgat cgaaccaggg
     7621 aaagagaacc gggaggacaa gcagtgtcaa tgtggtcgat gtcaggaggc cgctgatgac
     7681 gacgacgg
//
LOCUS       DATMTI010000482         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_102939, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000482 DATMTI010000000
VERSION     DATMTI010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102939"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..918
                     /locus_tag="VN039_07770"
     CDS             142..918
                     /locus_tag="VN039_07770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV45900.1"
                     /translation="MVKTDHRPLAKDSQAPAKVVSTWSGQAREDAVQRMFTAIARVYD
                     LNNTLLSFGLHYYWKKITASFITPVGRGAALDVGSGTADLALLVESRMGPQGRVIASD
                     LNHAMLAEGFKKIGGKGLTDKITCLQASAEHLGFGDNTFDAVTTGFCMRNVGDLPGAV
                     REIRRVMKPGGRFVCLEFSRPVFGWLRTFYDWYSFKLLPWIGTMVARDRTGVYEYLPA
                     SIRTFPDQERLCQILRDAGFRQVSYKNLSGGIVAVHIATK"
     gene            1004..>2238
                     /locus_tag="VN039_07775"
     CDS             1004..>2238
                     /locus_tag="VN039_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWV45901.1"
                     /translation="MNSILYIFLPCKKVYPIGITYLADFIHRRKPEVRQRILDLSLFP
                     VGQQSQAIRDAATEVKPDLVCFSWRDIQIFSPHEGDTSLEHAFNFYFASNPLKRIAAS
                     VEGVKQLYRYYNHIYTILSYPALIRKEFPKAQIMIGGGAFTAFADQLIERLPEGTIGI
                     LGEGEDAILKVIEGRSIEDERYIVKEGNQIRKGRQGSPALLDALTVDLPYLTSIFPQY
                     GEYIGESIGVQSKRGCPYDCAFCLYPYIEGKRVRYRPPDMVVKDIAQHYHQWGARRFW
                     FTDAQFITGKEAYPQCTEILERIVAEKLEIEWSGYIRTSLITPELAKLMVRSGVGDLE
                     VAITSGSQEVLNNLHMGFKLERLYDGCRYLAEAGFKGKVILNYSLNSPKETEESLLQS
                     VEAYKKVASILGEERVFPL"
BASE COUNT          501 a          645 c          601 g          491 t
ORIGIN      
        1 gttcaccgtc catgcaggca ggcattgcgg accaatgttt ggagcctgga agagctggcg
       61 ggattaagaa attgaaactg acccactacc gacctacccc tcaccttgac aaagcttgaa
      121 accgggtgct accaccctca catggtgaaa acagaccatc gtccattggc caaagactcc
      181 caggctcccg ccaaggtcgt ctcgacgtgg tccggccagg cccgcgagga tgccgtgcag
      241 cggatgttca ccgccattgc acgcgtctac gatctgaaca acacgctgtt gagcttcggc
      301 ctgcattact attggaaaaa gattaccgct tccttcatta ctccggtcgg acggggggcg
      361 gcgctcgatg tcggatccgg tacagccgac ctcgcgctcc ttgtcgaatc tcgaatggga
      421 ccccaggggc gcgtgatcgc atcagacctg aaccacgcga tgctggccga gggtttcaag
      481 aagatcgggg gtaagggcct caccgacaag atcacttgcc tccaggcgag cgcggaacat
      541 ctgggatttg gagacaatac cttcgatgcg gtcaccaccg gtttctgcat gcggaacgtc
      601 ggagatttgc cgggagccgt gagagagatc cgtcgcgtga tgaaacccgg cggccgcttc
      661 gtgtgcctgg agttttcacg tccggtattc ggttggttgc gaacgttcta cgattggtat
      721 tcattcaaac tgctgccctg gatcggcacc atggtcgccc gcgaccgcac cggcgtctat
      781 gagtaccttc cggcatccat ccggacgttt cccgaccaag agcgattgtg ccagatcctt
      841 cgcgatgccg ggtttcgtca ggtgtcatac aagaacctca gcggcggtat tgtcgccgtt
      901 catatagcga ccaaatgaag agcttctagc ttatggccta tagcacaagt ccgaagaggc
      961 ggatcctttt tctgctatac gccattagcc atcggctcag actatgaatt ccatcctcta
     1021 cattttcctt ccctgcaaga aggtctaccc catcgggatc acctacttgg cggactttat
     1081 tcatcggcgc aagccggagg tccgtcagcg cattctcgat ctctcattgt ttccagtggg
     1141 gcagcaaagc caggccatcc gcgatgcagc cacggaggtc aagccggacc tggtctgctt
     1201 ttcttggcgc gatattcaga ttttttcgcc ccatgaaggc gacacctcgc tggagcatgc
     1261 gttcaatttt tattttgcca gtaaccccct caagcgcatc gcggcgtcag tcgagggtgt
     1321 gaagcagctc taccgctact acaaccacat ctatacgatc ctgtcgtacc ccgcattgat
     1381 ccgcaaagag ttccccaagg ctcagatcat gatcggaggg ggggcgttta cggccttcgc
     1441 cgatcaattg atcgaaagac tcccggaagg aaccatcggc atcctcggtg aaggagaaga
     1501 cgccatactc aaagttatcg aaggccgatc catcgaggat gagcggtaca tcgtcaaaga
     1561 aggcaaccag attcggaaag gccgacaggg ttcgccggcg ctgctcgacg cgctgaccgt
     1621 cgatttaccc tacctcacgt cgatctttcc tcagtatggt gaatatatcg gcgaatccat
     1681 cggggtacag tcaaaacgag gctgccccta cgactgcgcc ttctgcctct atccctatat
     1741 tgaaggcaag cgagtccgct atcggccccc tgacatggtc gtcaaggaca ttgcccaaca
     1801 ttatcaccag tggggtgccc gtcgattctg gtttaccgac gcccaattca tcactgggaa
     1861 ggaagcctat cctcaatgca cggagatcct cgagcggatc gttgccgaga aacttgagat
     1921 cgagtggtcc ggctacatca gaacctcgct gattaccccg gaactcgcga agctgatggt
     1981 ccggtcaggc gtgggcgatc tcgaagtagc cattacgtca ggctcgcaag aggtgctgaa
     2041 caatctgcat atgggattca agctggaacg cctctatgac ggctgtcgtt atctggccga
     2101 agcaggcttt aagggcaagg tgatcctgaa ttactccctc aatagtccaa aagaaacgga
     2161 agagagcctt ctccagagcg tggaggccta caagaaggtc gcctcaattc ttggagaaga
     2221 acgcgtattc cccttgat
//
LOCUS       DATMTI010000483         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_527983, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000483 DATMTI010000000
VERSION     DATMTI010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527983"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..623
                     /locus_tag="VN039_07780"
     CDS             123..623
                     /locus_tag="VN039_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45902.1"
                     /translation="MSYKCGYCKGRHQKLETVKRCALEAYAERAAHQVDEEPEPLKVG
                     PASRSLAILPAGWYSIWTGSEPTVVVEIRKPASGKWKGRYFVNCTQNGKTEAIFDKAD
                     RDKVISDLLAVDFGMYMMLYGKQTGLCPICHEPLSDTEKDIGVHLGTADEKVNTTRDC
                     YKAITE"
     gene            633..872
                     /locus_tag="VN039_07785"
     CDS             633..872
                     /locus_tag="VN039_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45903.1"
                     /translation="MATTAAKVKPVKKAAAKSAEPQRPVDVDLVFEIERTTSNTYRFK
                     EVAEPGEEVIGPLYVKKHHFGEFVPSSLTVTVRVD"
     gene            899..967
                     /locus_tag="VN039_07790"
     tRNA            899..967
                     /locus_tag="VN039_07790"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1195..1728
                     /locus_tag="VN039_07795"
     CDS             1195..1728
                     /locus_tag="VN039_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45904.1"
                     /translation="MPKQVRLRVPSMTPERTVQIFLEALAEGTFDTHLDILFQAIDGR
                     IQESQQEPGDAVEPRDRVPGYIPVDLVVGKHYGVLGEKYSGVAVMYIEDLEPRDGDGG
                     IPKVIVECITGNENVISGKRYKLPVSALTEIVEDIKPVPHRPPYGYARGNCRTCGGPV
                     EYNGKGRPPSVCAACRN"
     gene            1743..2741
                     /locus_tag="VN039_07800"
     CDS             1743..2741
                     /locus_tag="VN039_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV45905.1"
                     /translation="MASNGQVLRVAKPLAKVRVTEPVVAMEATEDLSQRHAFIPDPKY
                     AKNYVHRKVNGVQDFDLLDYAMSKGHNILLMGDTGAGKTMLPMAYAAQKGLAYYSVPC
                     DVSIDPSALFGKMMPGTEIGKFVWVDGPVTEMVRNGGVLNISEVNFMPARIASSLYPL
                     LDHRRSIQLLGHQGESIRAHPDLLIVADMNPKYRGTQELNLAFANRFPIKVVWGYDPV
                     VEEKLVPSKALLDMVRKIRNEAGIQTPIGTNAMLEFVELFGSLGFKFAKSNFTNGFRD
                     TERGAVDKILDASSVNISNELSKMHQDIDKEIEELNDKGWFDPKSSNPEDFDMEEV"
     gene            2745..>3139
                     /locus_tag="VN039_07805"
     CDS             2745..>3139
                     /locus_tag="VN039_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45906.1"
                     /translation="MGSVPVSKALTSREDELKTRLEALRSTLQLTDRVLTGDSRVTLR
                     FCYGDLRGSNCPAMSVGPAITVDIDQVPNPHAKSGLVHILGLNYHEVGHVRYGIDRRQ
                     LRNRVQGRLHQRFDEAYEILEEGRIETLLG"
BASE COUNT          725 a          798 c         1014 g          602 t
ORIGIN      
        1 gtgagatggt ggataccctg aatcttcttc agaggaagat cgcggaacga aagaactgaa
       61 cagcttgcgg gctgggcagg gtgataccct gttcggtctg ctggcctttc agggaggatg
      121 tgatgagcta caagtgcggc tactgcaagg gccgacacca gaagttggaa accgtgaagc
      181 gttgtgcgct tgaagcgtac gcggagagag cagcacacca agtggatgaa gaacctgagc
      241 cgctgaaggt cggaccggct tctaggtcgt tggcgatcct tcccgcaggt tggtactcca
      301 tttggaccgg ctctgagccg actgtggtgg ttgagattcg caaaccggct tccggaaagt
      361 ggaaggggcg gtacttcgtc aactgcacac agaacgggaa gactgaagcc atcttcgata
      421 aggcggaccg agacaaggta atctcggatc tgctcgcggt tgatttcgga atgtacatga
      481 tgctctacgg aaagcagacc gggctgtgcc cgatttgcca tgagccgctg agcgacacgg
      541 agaaggacat cggcgtgcat ctcggcacgg cggatgaaaa agtaaacaca acccgtgatt
      601 gctacaaggc aatcaccgag taggaggaag aaatggcaac gacggcagcg aaggtgaagc
      661 cggtgaagaa ggctgcggcg aagtctgcgg aaccgcagcg gccggtggat gtggacctgg
      721 tgttcgagat cgagcgcacc acgtccaaca cctaccgctt caaggaagtc gcagagccgg
      781 gcgaggaagt catcggcccg ctctacgtca agaagcacca cttcggcgag ttcgttccgt
      841 ccagcttgac tgtcacggtc cgggtggact aagcacggcg gggaggaaac tcccctatgg
      901 gcgcgtagct caattggcag agcgcgtgag gctagcgagg ttgcaggttc gaaccctgcc
      961 gtgcccactg gataacttag acgaagggaa aatgatgagc ccggaacttc ctaagccgaa
     1021 tgagtgcact ccgatcgtaa gctggaaaat ccagtggatc agtaagcagg ggtggatgaa
     1081 cgcgccgatc ttccgcaggg atcaggcgga cgcgatcgac ctggctcgac tccagcgcgc
     1141 tgcgggtttc aagaccaagc tgttccgcgt tgaggaaacg gagatcgact tctgatgccg
     1201 aagcaggtaa ggctgcgagt tccttcgatg acgccggaaa ggacggtgca gatatttctg
     1261 gaagctctgg cggaaggcac gttcgacacg catctggaca tcctctttca ggcaatcgac
     1321 ggtcgcatcc aggagtcgca gcaagagcca ggggatgccg tagagccgcg tgaccgtgtg
     1381 ccgggttaca ttccggtgga tttggtcgtg ggcaagcatt atggggttct tggggagaag
     1441 tactccggtg tcgcagtgat gtacatcgaa gacctggagc cgcgtgacgg tgacggcggc
     1501 atcccgaaag tcatagtcga gtgcatcacg gggaacgaaa atgttattag cggcaagcgg
     1561 tacaagcttc cggtttccgc gttgactgag attgttgaag acattaagcc ggttcctcat
     1621 aggccaccct acggatacgc cagaggaaac tgccgtacgt gcggcggacc ggtcgagtac
     1681 aacggcaagg gtcggccgcc gtcggtctgc gccgcttgta ggaactagac ggaaagaagg
     1741 atatggctag caacgggcaa gttctgcgag tggccaagcc gctggcgaag gtgcgggtaa
     1801 ctgaaccggt tgtcgcgatg gaggcgacgg aggatctgag tcagcggcac gcgttcattc
     1861 cagatccgaa gtatgcgaag aactacgttc atcggaaggt gaatggagta caggacttcg
     1921 atctgctcga ctacgccatg agcaaggggc acaacatcct gctcatggga gacacgggcg
     1981 cgggaaagac aatgctcccc atggcctacg cggctcagaa gggactggct tactattcag
     2041 tcccctgtga tgtctcgatc gatccctccg cgttgttcgg caagatgatg ccgggaacag
     2101 agatcgggaa gttcgtgtgg gtggacggac cggtcacgga gatggtccgc aacggcgggg
     2161 tgctgaacat ctcggaagtg aacttcatgc cagcgaggat cgcctcttcg ctctatccgc
     2221 tgttggacca tcgccgttcg atccagcttc tcggacacca gggtgaaagc atccgggcgc
     2281 atcctgatct gctcatcgtg gcggacatga acccgaagta ccgggggaca caggagctca
     2341 acctcgcttt cgccaaccgg ttccccatca aggtggtgtg gggatatgac ccggttgtgg
     2401 aagagaagct ggttccttct aaggcgttgc tggacatggt tcggaagatc cgcaatgaag
     2461 cgggaatcca gacgccgatc ggcaccaacg cgatgcttga attcgtcgag ctgttcgggt
     2521 cactgggctt caagttcgcg aagtcgaact tcacgaacgg attccgtgac actgagcgtg
     2581 gcgcggtgga caagatcctg gacgcttctt cggtgaacat ctcgaacgag ctgtccaaga
     2641 tgcatcagga catagacaag gaaatcgagg agctgaacga caagggctgg ttcgatccga
     2701 agtcgtcgaa cccggaagac ttcgacatgg aggaggtgta actcttgggc tcagtgccgg
     2761 tcagcaaggc gcttaccagt cgcgaagacg agttgaagac gcggctcgaa gcgctgcgtt
     2821 ccacactcca gctcactgac cgagttctta ccggagactc cagggtgacg ttgaggttct
     2881 gctacgggga cttgcggggt tcaaactgtc cggccatgtc ggtaggaccg gcgatcacgg
     2941 tggacatcga tcaggttcct aacccgcacg ctaagtccgg tttggttcac atccttggac
     3001 tgaactacca cgaagtaggc cacgtccgtt acggcatcga tcgccgtcag ttgcggaaca
     3061 gagtgcaggg gagactgcac caacgtttcg atgaggctta cgagatcctt gaagaaggcc
     3121 ggatcgaaac ccttctggg
//
LOCUS       DATMTI010000484         1815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_60482, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000484 DATMTI010000000
VERSION     DATMTI010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60482"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..585
                     /gene="gcvH"
                     /locus_tag="VN039_07810"
     CDS             196..585
                     /gene="gcvH"
                     /locus_tag="VN039_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWV45907.1"
                     /translation="MIPSDLRFHKEHEWIRLDGKRATVGISDFAQDALGDIVFLELPK
                     VGVSVKVGQQIGEVESTKTTSTIYTPVSGTVSQINTALKDNPEVVNSDPYGKGWMAVI
                     ELADSGEVDRLMTASQYEAFLASQKKG"
     gene            836..1291
                     /locus_tag="VN039_07815"
     CDS             836..1291
                     /locus_tag="VN039_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWV45908.1"
                     /translation="MIKSLFLKLGMLSLTIGLVIWVRWTPQPPAQGIPSAPEQPIIAS
                     QATELEERNSRTVGSSSQNVQTPNMKAVALTEPSKTAVHSRLDLNRASAGELESLPGI
                     GAVLAQRVIAFRESAGGFRTVEDLRDVKGIGAKTFDRLKSLVTVSMAKS"
     gene            1315..>1815
                     /gene="tyrS"
                     /locus_tag="VN039_07820"
     CDS             1315..>1815
                     /gene="tyrS"
                     /locus_tag="VN039_07820"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWV45909.1"
                     /translation="MKAINEQLELIQRGAVEIIQQVELETKLKRAVSEHRPLRVKAGF
                     DPTAPDLHLGHTVLIHKLKHFQDLGHQVIFLIGDFTGMIGDPTGVSETRKALTKEQVL
                     DNAKTYQRQIFKILDPAKTLIEFNSRWMKEMSAEGLIQLAAHYRVARIMERDDFHKRH
                     QEQKPIS"
BASE COUNT          451 a          463 c          485 g          416 t
ORIGIN      
        1 attttcagct gaccgtcctg tgttgaagcg cgaggatctc ctcgcgctct ccacctctga
       61 ccggtacgat cgttccttga caacgccggt ccgccccgac taccatccgg aagaatcttt
      121 caggttgagg ttgagcagta ggttcccttt ccttgacctc aacctctatc actgatttcc
      181 ccaaacggag ccgacatgat tccgtccgat ttacgattcc acaaagagca tgaatggatt
      241 cgcctagatg ggaaacgagc gactgtgggt atcagcgact ttgcgcagga tgctttaggc
      301 gatatcgttt ttctggaatt gcctaaagtg ggcgtttcgg tcaaagtcgg tcaacagatc
      361 ggcgaggtcg aatcgacaaa aacgacctct acgatttata cgccggtaag cggaacggtg
      421 agccaaatca atactgccct taaagataat cctgaagtgg tgaactccga cccctacggc
      481 aaaggttgga tggccgtgat cgagcttgcc gattctgggg aagtcgatcg actgatgacg
      541 gcttcgcaat atgaggcctt ccttgccagc cagaagaaag gttaaggctt aggtcgaggt
      601 taagcgccgg ataatctctt ttatacttta taacctcgac ctgcgcgcct cacccttgct
      661 atgtccgatc aacccacatc ctgattctct gtgctggacc cggcggatat gtcgcggctc
      721 aagtggctgg gatgatccat gtgcatccgg cgcttgctga aggatatgat ggagaccatg
      781 gaggatgtcg gggagctccg ttatacacct tattcgttcg aaagaaggca agtgcatgat
      841 taagtcccta tttctgaaac tcggtatgtt gtccttgaca atcggtttgg ttatctgggt
      901 ccgatggact ccacaaccgc ccgctcaagg catcccatca gcgccagagc aaccgatcat
      961 cgcttcccag gctacggagt tagaagagcg gaacagcagg actgtcggct catctagtca
     1021 gaatgtacag actccgaaca tgaaggccgt tgcactgacg gagccatcaa aaacggcggt
     1081 tcattcgcga ctcgacctga atcgggcaag tgccggtgag ctcgaatcgt tgccaggaat
     1141 tggagcagta ctggctcaac gtgtgatagc ctttcgcgaa tcggccggag gctttcgtac
     1201 ggtcgaagat ctccgtgacg ttaaagggat tggggcaaaa acattcgatc gcctcaaatc
     1261 cttagtgacg gtctcgatgg cgaaatccta agagtgacgg agcaaacaaa gacgatgaag
     1321 gcgataaacg agcagctgga gctgatccag cgcggcgctg tagagattat tcaacaggtc
     1381 gagctggaga caaaactcaa gcgggctgtt tctgagcatc gtcccttgcg cgtcaaggcg
     1441 ggattcgatc cgacggcgcc ggatcttcat cttggacata cggttctgat tcataaattg
     1501 aagcacttcc aagatctcgg ccaccaagtc atcttcctca tcggtgattt caccggcatg
     1561 atcggtgatc caaccggcgt atccgagact cgtaaagcat tgaccaagga acaggtgctg
     1621 gacaatgcca agacctatca gagacaaatc ttcaagattc tcgatccagc aaagactctg
     1681 atcgaattca atagtcgatg gatgaaggaa atgtcggcgg aagggctcat tcagctggcc
     1741 gcgcattatc gagtggctcg cataatggag cgtgacgact tccataagcg ccatcaggag
     1801 cagaagccga tcagt
//
LOCUS       DATMTI010000485         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_782329, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000485 DATMTI010000000
VERSION     DATMTI010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782329"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..363)
                     /locus_tag="VN039_07825"
     CDS             complement(10..363)
                     /locus_tag="VN039_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45910.1"
                     /translation="MNAELNIKALDLQAEVTAQNFGAEGEMVFFTLTTVTGNAVTVRA
                     KRREMKTFLSDAGVWMGLALEPSVDTIPLIAAEHALRVFDGLPDNWTPSPSAVMHMHE
                     ALIAVLPALTRPSKD"
     gene            complement(353..700)
                     /locus_tag="VN039_07830"
     CDS             complement(353..700)
                     /locus_tag="VN039_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45911.1"
                     /translation="MKLKWKVQPEPTGKYRAFEKRGWPYCEDENGRTIAFIQCDDSYS
                     IRKAAGQDHSPLTVLIRCGATSPEERKLHGAFRWRKFKTQFSSLTLAKFHFEQFVKNN
                     PNHCWDYEDIRER"
     gene            complement(936..1139)
                     /locus_tag="VN039_07835"
     CDS             complement(936..1139)
                     /locus_tag="VN039_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45912.1"
                     /translation="MAYTREGKAQRLIARFHDAIVEKEIAQAKYDHMPTTVHHEAKSK
                     AIARFNMLRGTLYRIVVSSSEEL"
     gene            complement(1139..1390)
                     /locus_tag="VN039_07840"
     CDS             complement(1139..1390)
                     /locus_tag="VN039_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45913.1"
                     /translation="MSNMSYCRFHNTYHDFLDCASVLEEMADGEGHPLSEDELDAAVA
                     MLTRMEEIMTMLNEKRENRELDTMRELLESLNDNLKEGR"
     gene            complement(1387..1608)
                     /locus_tag="VN039_07845"
     CDS             complement(1387..1608)
                     /locus_tag="VN039_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45914.1"
                     /translation="MNIHAYGLVCYVSVPVDDAAAKMRLGKFAGERVLCTVEQIVLNN
                     GLGFSVYAVKLSGQYAEINRDREALENLS"
     gene            complement(1605..1841)
                     /locus_tag="VN039_07850"
     CDS             complement(1605..1841)
                     /locus_tag="VN039_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45915.1"
                     /translation="MESTYSLNVTQRNPSRYSIRGYILEDGIQIGWFRRPASPGFIPE
                     MQFKFFTEHAEARFQGFCDSLSMEETVAAMAGGV"
BASE COUNT          445 a          565 c          591 g          474 t
ORIGIN      
        1 gcatatcaat taatccttcg aagggcgcgt aagcgcgggt agaaccgcaa tcaatgcttc
       61 gtgcatgtgc atcaccgcag acggagaggg tgtccaattg tccggcagac catcaaatac
      121 acgcagcgca tgctctgccg caatcaatgg aatggtgtcg acagacggct ccaacgcgag
      181 tcccatccat acacccgcgt cagacagaaa cgtcttcatc tcgcgacgct ttgcgcgcac
      241 ggtgactgca ttgcctgtga ctgtggtcaa ggtgaagaag accatttcgc cttccgcgcc
      301 gaagttctga gcagtcactt cagcttggag gtccaaagct ttgatgttga gttcagcgtt
      361 cacgtatgtc ctcgtaatcc cagcaatggt tcggattgtt cttcacaaat tgttcgaaat
      421 ggaacttggc gagcgtcaag ctcgagaatt gtgtcttgaa cttacgccaa cggaacgcac
      481 cgtgcagctt tctttcctcg ggagaggttg caccacagcg aatcagaacc gtcagagggc
      541 tgtgatcttg tcccgcagct ttccggatcg agtacgagtc gtcgcactgg atgaatgcga
      601 tcgttcgacc gttctcgtct tcgcagtagg gccaaccgcg cttctcgaat gcgcgatact
      661 tgcccgtggg ctcaggctga actttccact tgagcttcac cgcggaaacc tctggccggc
      721 gccgtaccat ttcaagttca gattcaagga gttgagcttg aagctgaagt gatagtacag
      781 cagtcgttcc tcgcgagagc agaccctgtt caggttctcc cgcgattcct gcgagtacga
      841 ccagaacccg acccactcca gacggggctt cggcatgggg tgcgttatgt tgaacattgc
      901 ccggccgaac ctctgaagag cgacattcgc attcatcaca gctcctccga gctactgacc
      961 acgatgcggt agagcgttcc acgcagcatg ttgaagcgcg cgatcgcttt cgacttggct
     1021 tcgtgatgaa cggtcgttgg catgtggtca tacttggcct gtgcgatctc cttctcgaca
     1081 atggcgtcgt ggaagcgcgc aatcaggcgc tgggcttttc cttcgcgcgt gtaggccatc
     1141 acctgccctc cttcagattg tcattgagcg attcgagcag ttcgcgcatg gtgtcgagct
     1201 cacggttctc gcgcttctcg ttgagcatcg tcatgatctc ctccatgcgc gtgagcatcg
     1261 cgaccgccgc atcgagttcg tcttcagaca gagggtgtcc ttcgccgtcg gccatctctt
     1321 cgagaaccga agcgcagtcg agaaagtcgt gataggtgtt gtgaaagcgg caatacgaca
     1381 tattgctcat gacaggttct ccagggcttc gcgatcacgg ttgatctccg catactggcc
     1441 ggacagcttg accgcgtaca cggagaaccc gagaccattg ttcaggacga tctgctccac
     1501 ggtgcacagc acgcgctcac ccgcgaactt tccgaggcgc attttcgcag cggcgtcatc
     1561 aacgggtacg gacacgtagc agacgaggcc ataggcgtgg atattcatac gccacccgcc
     1621 attgctgcga ccgtttcttc catcgacagc gaatcgcaga aaccctggaa gcgggcttcc
     1681 gcgtgttcgg tgaagaactt gaattgcatc tcgggaatga accctggact ggcgggacga
     1741 cggaaccaac caatctgaat cccgtcctcc aaaatgtatc cccgaatcga ataccgagag
     1801 ggattgcgct gagtgacgtt gagcgagtat gtggattcca tgtttgagac ccttgttgac
     1861 cacagtgagg aggcggagtg cagcggttgc gcatggtacg tggaacggta caggtagcgg
     1921 gcgcattggg cggcgcattg cgtactgtgg agctactata acaggcgtta ggcatgggcg
     1981 caatgacttt ttgctggtac gtgcaaagct catcgggagg ggcgaaaaga actgagtttc
     2041 gaatttgaga ggcgttttta cagggtagga tagag
//
LOCUS       DATMTI010000486         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_442719, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000486 DATMTI010000000
VERSION     DATMTI010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442719"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..2194)
                     /gene="kdpB"
                     /locus_tag="VN039_07855"
     CDS             complement(74..2194)
                     /gene="kdpB"
                     /locus_tag="VN039_07855"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HWV45916.1"
                     /translation="MSMPTKAHKSLFDAAVVRLACRDAVVKLDPRHQVKNPVMFVVWV
                     GSVLTTLLFLQAMAGAGEAPTWFVFSIALWLWFTVLFANFAEAMAEGRGKAQADSLRR
                     ARRELTAKRLGAVDPGSEHYAVWNRQFRRRDTFSVVSANQLKQGDVFLVEAGDFIPAD
                     GEIIEGVASVNESAITGESAPVIRESGGDRSAVTGGTKVLSDRLIVRVTASPGESFLD
                     RMIAMVEGAKRQKTPNEIALTILLAALTIIFLLATVTLLPFSLFAVQASGQGTPVAVT
                     VLVALLVCLIPTTIGALLSAVGIAGMDRMVQANVIAMSGKAVEAAGDVDVLLLDKTGT
                     ITLGNRQATAFLPEEGIDIQTLADAAQLSSLADETPEGRSIVVLAKEKYGLRARDIRE
                     IGAKFIPFTAQTRMSGVDVDGREIRKGSDDAVEAYVTSKGGHFSQFVNSLVETIAKQG
                     GTPLVVAENVKVLGVISLQDVVKGGIKERFGELRRMGIKTVMITGDNPLTAAAVAAEA
                     GVDDFLAQATPETKLKLIRDMQAEGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAK
                     EAGNLVDLDSNPTKLIEIVEIGKQLLMTRGALTTFSIANDVAKYFAIIPAAFATTYPV
                     LNILNVMGLATPQSAVLSAVIFNALIIVALIPLALRGITYRPIGAELLLRRHLLIYGL
                     GGVAMPFVGIKVIDLILVALRVAG"
     gene            complement(2209..>4008)
                     /gene="kdpA"
                     /locus_tag="VN039_07860"
     CDS             complement(2209..>4008)
                     /gene="kdpA"
                     /locus_tag="VN039_07860"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWV45917.1"
                     /translation="CRSADLSHGRVAEGGVVLMTMNAILQVLVFFVVLLALAKPLGWY
                     MARVYEGKPCGLDRFLGPVERIMYRLCGVRPTDEMDWKNYGLAMLLFNAAGFLFLYGL
                     QRLQGLLPLNPANLGAVPPDLAFNTAASFVTNTNWQAYGGEATLSYLTQMLGLTVQNF
                     VSAATGMAVLIALIRSLRRSTSRALGNFWSDLVRSTLYILLPLALILSFLLVSQGVVQ
                     TVASYHSATPLQQSKPDTTPATEIGRLLAVGPAASQIAIKQLGTNGGGFFNANSAHPF
                     ENPTPLSNFLEVLALVLIPAAFCYTFGKMVGDTRQGWVILAAMTILLLCFVPLGLWAE
                     QSGNPLLSDIGVDQQSQAGQAGGNMEGKETRFGITGSALWSAVTTAASNGAVNSMHDS
                     YTPMGGLVPMFLMQFGEVVFGGVGSGLYGMIVFAIVAVFVAGLMVGRTPEYLGKKIEP
                     YEMKMAALLILIMPIVVLGFTALAVGTETGRSSILNPGPHGFSEILYAYTSQGNNNGS
                     AFAGLNANTPFYNLTGGIAMLISRFWLAIPILALAGALARKKLVPAGPGTLPTHTPLF
                     VVLLIGVVVMVGALTFLPALALGPIVEELMMRG"
BASE COUNT          908 a         1190 c         1107 g          803 t
ORIGIN      
        1 ggctaagatt gaggttacgg tcgagaaaga gaaagctcct ccttctgatc aaccttaacc
       61 tcaacctgct tcatcaaccc gcaacgcgca gggcgacgag aatcaggtcg ataaccttaa
      121 tgccgacaaa tggcattgcc acaccgccca atccgtaaat caggagatgc cgccgcagca
      181 gcaattctgc tccaatgggt cgatacgtga tgcctcgtag cgcaagcgga atgagcgcaa
      241 cgatgatcag cgcgttgaaa atgacagccg acaggaccgc gctttgaggc gtggccagcc
      301 ccatcacgtt caagatgttg agcaccggat aggtcgtggc gaaggccgca ggaatgatgg
      361 caaagtattt ggccacatcg ttggcgatac tgaaggtcgt gagcgcgccg cgtgtcatga
      421 gcaattgttt gccgatctcc acgatttcga tgagcttagt gggattggaa tccagatcga
      481 ccagattgcc cgcttctttg gcggcctgcg ttccggtgtt catcgcaacc gcaacatcag
      541 cttgagccaa cgcaggcgca tcgttcgtac catctcccgt catggccacg aggcggcctt
      601 cagcctgcat atcgcggatc aatttcagtt tggtctccgg agtggcttgc gcaagaaaat
      661 catccacccc ggcttccgcc gctacggccg ccgccgtcaa gggattgtcg ccggtgatca
      721 tgaccgtttt gatgcccatg cgccgcagct cgccgaaccg ctctttgatc ccgcccttga
      781 cgacatcttg tagactgatc actccgagca ctttgacatt ctctgccacg acgagaggag
      841 tcccgccttg cttcgcgatt gtctccacaa gagaattcac gaattgcgag aagtgcccgc
      901 ctttcgatgt gacataagct tcaacggcat cgtccgaacc cttgcggatt tcccgtccgt
      961 ctacatcgac tccactcatg cgggtttgtg ccgtaaatgg aatgaatttg gcgcctatct
     1021 cacggatatc ccgcgcgcgt aatccatact tctctttggc caggacgacg atgctgcggc
     1081 cctccggcgt ctcgtccgcc aacgatgaga gttgggctgc gtcggccagc gtctggatgt
     1141 ctattccttc ctccggaagg aaggcggtcg cctgacggtt tcccagcgtg atcgtgccgg
     1201 tcttatccag cagcaggacg tccacatccc ctgccgcttc aacggccttt cccgacatcg
     1261 cgatgacgtt ggcctgcacc atgcggtcca tgccggcaat tccaacggcc gagagaagag
     1321 caccaatggt tgtcgggatc aggcagacca agagtgccac caacactgtg accgccaccg
     1381 gcgtcccctg tcccgaggcc tgcacggcga atagagagaa cggcaacagg gtcactgttg
     1441 ccaagagaaa gatgatggtc agggctgcga ggagaatggt gagggcgatt tcattcggcg
     1501 ttttctgtcg tttggctcct tccaccatgg cgatcatccg atccagaaag ctttctcctg
     1561 gactggccgt gacacgaacg atgagccggt cggatagcac ttttgttcca cctgtgacgg
     1621 cgctgcggtc gccgccgctt tctcgaatga cgggcgcgct ctcgccggtg atggcgctct
     1681 cattcaccga cgccacgcct tctatgatct cgccatcggc ggggatgaag tctcccgcct
     1741 cgactaggaa tacatctcct tgcttgagtt gattcgccga caccacgctg aacgtatcgc
     1801 gccgccggaa ctgtcggttc cacacggcat aatgctcgct tccagggtcg acggcgccaa
     1861 gcctcttggc cgtgagttct cgacgcgcgc gccggagcga atcggcttgc gctttgcccc
     1921 gaccttccgc catggcctcc gcaaagttgg caaacaggac ggtgaaccag agccagagag
     1981 caatggaaaa aacaaaccaa gtcggcgcct cccccgctcc ggccatcgcc tgcagaaaca
     2041 gaagggtcgt caagacactt cctacccaca ccacgaacat gaccgggttc ttgacttgat
     2101 ggcgaggatc gagtttgaca acggcgtcgc ggcacgccag gcgcactact gcggcgtcga
     2161 aaagagactt gtgtgctttt gtcggcatac tcatcgtgtg attccttact accccctcat
     2221 catcagctcc tcgacgatcg gccccaaggc tagagcaggt agaaacgtga gtgccccgac
     2281 catcactacg acgccgatca agagcaccac gaacaatggc gtgtgagtcg gcaaagtccc
     2341 ggggccggcc ggcactagtt tctttcgcgc caaggcacct gcgagtgcca gaatcggaat
     2401 agccagccag aatcgggata tcagcatcgc gatcccaccg gtgaggttgt agaaaggagt
     2461 gttcgcattg agccccgcaa acgcgctgcc gttgttgttc ccctgggacg tataggcgta
     2521 aaggatctcg ctgaatccat gtggaccggg attcagaatg gaagatcgac ctgtttcggt
     2581 gccgacggcc aaagcggtga agcccaagac aacgatgggc atgatgagaa tgaggagcgc
     2641 ggccattttc atctcatagg gttcgatctt cttccccagg tactccggtg tccgtccaac
     2701 catgagtccg gcaacaaaca cggcgacaat cgcgaagacg atcatgccgt ataggcctga
     2761 gccgactccg ccgaagacta cttcgccgaa ctgcatcagg aacatcggca ccagtccgcc
     2821 catcggcgta tacgaatcgt gcatcgagtt caccgctcca ttggaggcgg cagtcgtgac
     2881 cgcagaccac aaggccgacc cggtgatgcc gaatcgtgtc tccttccctt ccatattgcc
     2941 gccggcttgg ccggcttgag actgctgatc cacaccgata tcggaaagca atggattgcc
     3001 gctttgttcg gcccatagtc cgagaggaac aaaacagagc agcagaatgg tcatggcagc
     3061 gagaatgacc cacccttgac gggtatctcc gaccattttg ccgaacgtat agcagaacgc
     3121 tgcgggaatc aggacgaggg ccaagacttc aagaaaattg gacagcggtg ttgggttctc
     3181 aaacggatgg gcggaattcg cattgaaaaa tccgccgccg ttcgtgccga gttgcttgat
     3241 ggcaatctgg gaagcagccg gacccaccgc aagcagccgc ccgatttccg tggcaggagt
     3301 tgtgtcaggt ttgctctgct gtaacggcgt cgcactgtga tacgacgcaa cggtctgaac
     3361 cacaccctgg gaaaccagga gaaaagataa gatcagagca agcggcaaaa gaatatacag
     3421 tgtgctgcga acgaggtcgc tccagaaatt tccaagcgcc cttgacgtgc tccgtctcaa
     3481 gctccggatc aatgcaatga gcactgccat tcccgtcgcg gcggagacga aattctggac
     3541 ggtcagacca agcatttgcg tgagataact cagggtggct tctccgccat acgcctgcca
     3601 gttcgtattg gtcacgaaac tcgctgccgt gttgaaggcc aggtccggtg ggacggcacc
     3661 aaggttggcg gggttgagag gcaaaagacc ttgcagccgc tgcaagccat agaggaacag
     3721 gaatccagct gcattgaaga gcagcatggc caacccgtaa ttcttccagt ccatttcatc
     3781 ggtcggtcga acgccgcaga gtcgatacat gatccgttcc actggaccaa ggaaacggtc
     3841 caagccacag ggcttgcctt catagactct cgccatgtac cagcctaaag gcttcgccag
     3901 agccagcagc acgacgaaaa acacaagtac ttggaggatg gcattcatcg tcatcagaac
     3961 cactccgcct tcagcaacgc gaccatgaga tagatcagca gacctaca
//
LOCUS       DATMTI010000487         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_188954, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000487 DATMTI010000000
VERSION     DATMTI010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188954"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1591
                     /locus_tag="VN039_07865"
     rRNA            44..1591
                     /locus_tag="VN039_07865"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          425 a          389 c          505 g          341 t
ORIGIN      
        1 aaactttggt cgagcggatg ctgatccgtt cgccctccaa gttttaacgg agagtttgat
       61 tctggctcag aacgaacgct ggcggcgtgg ataagacatg caagtcgaac gagacttttc
      121 ctggtagcaa taccgggaaa agtttagtgg cgaacgggtg cgtaacacgt gggcaatctg
      181 ccttaaagtg ggggatagct cggcgaaagc cgaattaata ccgcatgtga ttaccggaga
      241 catcttcgga acatcaaagt tggtgcaagc taacgctttt agaggagccc gcggcctatc
      301 agctagttgg tggggtaacg gcctaccaag gcggtgacgg gtagctggtc tgagaggacg
      361 accagccaca ctggaactga gacacggtcc agacacctac gggtggcagc agtcgagaat
      421 ttttctcaat gggcgaaagc ctgaaggagc gacgccgcgt gggggatgaa tggcttcggc
      481 ccgtaaaccc ctgtcattcg cgatcaaacc ttattattta aaagatgatg agctgatagt
      541 agcggaagag gaagggacgg ctaactctgt gccagcagcc gcggtaatac agaggtccca
      601 agcgttgttc ggattcactg ggcgtaaagg gtgcgtaggc ggtcgggtaa gtctgatgtg
      661 aaatcacaca gcttaactgt gaaactgcat tggatactct ccggctagag gaatggaggg
      721 gagactggaa tacttggtgt agcagtgaaa tgcgtagata tcaagtggaa caccagtggc
      781 gaaggcgagt ctctggacat ttcctgacgc tgaggcacga aagccagggg agcaaacggg
      841 attagatacc ccggtagtcc tggccctaaa cggtgcgcgt ttgctgtaag tggaatcgac
      901 cccacttgtg gcgtagttaa cacgttaaac gcgccgcctg ggaagtacgg ccgcaaggtt
      961 aaaactcaaa gaaattgacg ggggcctgca caagcggtgg agtatgtggc ttaattcgat
     1021 gcaacgcgaa gaaccttacc tggccttgac atgcaagtgg tagaaccatg aaagtgggac
     1081 gaccccgcaa ggggagcttg cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga
     1141 tgttgggtta agtcccgcaa cgagcgcaac ccctgtgtcc tgttgccacc ccttaactgg
     1201 gagcactctg gacagactgc ctcgcttcaa cgaggaggaa ggtggggatg acgtcaagtc
     1261 aggatggccc ttacggccag ggctgcacac gtactacaat gcccggtaca aagggaagca
     1321 agaccgcaag gtggagcaaa tctcctaaaa ccgggcccag ttcagattgt cgtctgcaac
     1381 tcgacggcat gaagctggaa tcgctagtaa tggcgcatca gctacggcgc cgtgaatacg
     1441 ttcccaggcc ttgtacacac cgcccgtcac atcatgaaag ccgtttgtac ccgaagtcgc
     1501 ccctcaaaag gtgccgaagg tatgactggt gattgggatg aagtcgtaac aaggtagccg
     1561 taggggaacc tgcggctgga tcacctcctt tctaaggagt aattgcagag atttagaatc
     1621 gagaccgcaa ggtcgacatt caaattctct tgcgataggt
//
LOCUS       DATMTI010000488         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_103135, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000488 DATMTI010000000
VERSION     DATMTI010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103135"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VN039_07870"
     CDS             <1..760
                     /locus_tag="VN039_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV45918.1"
                     /translation="RVAELERQMVQKENQLSTLLGRNPGQIARGRSLTDQVLPPNVPA
                     GLPSELLQRRPDLVQAEQDLAAVTARIGAAKADRFPKLTITGILGVASPQFSRLIANE
                     TSFGVAGPSLAGPLLNAQILGFQQEAVEAQARQALAQYEQSILVAFREVENALVAVRT
                     TREQRDAQAEQVESLQSAFRQATLRYKSGLAHYLDVLIAQRNLFEAELSLTNTHRLHL
                     VSIVQLYKALGGGWSPNDSDQQMPAAKVVKALKG"
     gene            895..1653
                     /locus_tag="VN039_07875"
     CDS             895..1653
                     /locus_tag="VN039_07875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV45919.1"
                     /translation="MRLEHKTAIVTGGGTGIGEAIAKVFAREGAKVAITGRRKDELER
                     VVGDIERKGGRALAIPGSVTNEQDVQEAVAATVRAFGRLDILVNNAGNLFHAARLHET
                     TDQIWDETFDVFMKGTFRFIRAAIPRMLQQGGGSILNISTVGGLKAIRGFEAHAYQAA
                     KAGVIMLTKTVAVHYAKQNIRCNCICPAGVITPPIAGMLKDPQIKTWFEGMHPTGRLG
                     QPEEVAEATVYFASDESRWTTGSILSVDGGVMAE"
     gene            1788..>1991
                     /locus_tag="VN039_07880"
     CDS             1788..>1991
                     /locus_tag="VN039_07880"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HWV45920.1"
                     /translation="MMIDLLLSAFHFLLVFTLVAILAAQSAVIRPGITASSLRLAADL
                     DRGYGASALLLLGVGFSRVYWGAK"
BASE COUNT          447 a          551 c          605 g          388 t
ORIGIN      
        1 acgagtggcg gagttggagc ggcaaatggt acagaaagaa aatcagctga gcacgctcct
       61 aggacgtaac cccggtcaaa tcgcccgcgg ccggtccctg acggatcaag tgctgccgcc
      121 caacgtacca gccggactcc catcggagtt gctgcaacgg aggcccgacc tcgtacaagc
      181 cgaacaggac ttggcggcgg tgacggcgag aatcggtgca gccaaggcgg accggtttcc
      241 aaaactcacc attaccggca ttttgggcgt cgccagtccg cagttttccc gattgatcgc
      301 gaacgaaact tcgttcggcg tcgccggtcc cagtctggcc ggtcccttgc tgaatgcaca
      361 aattctcgga ttccaacagg aagccgtcga agcgcaagca aggcaagccc tggcccagta
      421 cgagcagtca atcctcgtcg cgttcagaga agttgagaat gccttggtcg ccgtacggac
      481 cacccgagag cagcgggatg cgcaagccga acaggtcgag tcgcttcaat ccgcctttcg
      541 acaagcgacg cttcgctata agagcggact cgcccattat ctggacgtgt tgatcgccca
      601 gcgcaacctg ttcgaggccg aactgtcgtt gacgaacaca caccgtctcc atctcgtttc
      661 gattgtgcaa ctgtacaaag ccctgggagg gggctggtcg ccaaacgatt cagatcaaca
      721 gatgccggcg gccaaagtcg tcaaagcact gaagggttga gggaactgaa aggcagagta
      781 tttcgcaccg gtgcgaatca gtgggacgct catcctggat ttcgatgacc acgtcgtcat
      841 gcgagaggtt ctagtcattg tatcaacggt tagccagacc gcttaggagg acacatgaga
      901 ctcgaacata agacagcaat cgtcaccggt ggaggaactg ggatcggcga ggcgattgcg
      961 aaggtttttg ctcgggaagg cgcgaaggtc gcgatcaccg gccggcgaaa ggatgaattg
     1021 gagcgcgtcg tcggagatat tgaacgaaag ggcggacggg ctttggcgat tcccggcagc
     1081 gtgaccaacg aacaggacgt gcaggaagcg gtggcagcaa cggtccgagc cttcggccgt
     1141 ctcgacatcc tggtcaataa cgcgggcaac ttattccacg ctgcgcggct ccacgagacg
     1201 actgatcaga tttgggatga gacctttgat gtgttcatga aggggacgtt tcgattcatt
     1261 cgcgcggcaa ttcctcggat gctgcagcaa ggcggcggct ccatcctgaa tatctccaca
     1321 gtcggcgggc tcaaggccat ccgtggtttt gaggctcatg cctatcaagc ggccaaggca
     1381 ggagtcatca tgctcaccaa gacggtggcg gtgcactacg cgaagcagaa catccgatgc
     1441 aactgcatct gcccggcagg cgttattacc ccacccatcg caggcatgtt gaaagatcca
     1501 cagatcaaaa cttggttcga aggcatgcat ccgacaggtc gactcggaca gccggaggag
     1561 gttgctgaag cgacggtgta cttcgcatcc gacgagtccc gctggacgac cgggagcatc
     1621 ctgtcggtgg acggcggcgt gatggccgag tgagcgatca gagatcaaag aaagcgagcg
     1681 acggtggaag tcaagcgctt ctctgaaccg cgcccccgcc gtcaatgtct aaatagtttg
     1741 tgatccattg gtccagcgtt tcacgatgtc cggctggaaa agcgcgaatg atgatcgact
     1801 tactcctttc tgcctttcac ttcttgctgg tctttacgct ggttgccatc ctcgccgcac
     1861 agagtgccgt gatcagaccg gggataaccg cttcgagcct gcgtctagct gccgacttgg
     1921 atcgaggcta cggtgcaagc gcgttgttgc tgctcggagt gggattcagc cgggtctatt
     1981 ggggcgcaaa g
//
LOCUS       DATMTI010000489         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_189046, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000489 DATMTI010000000
VERSION     DATMTI010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189046"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VN039_07885"
     CDS             <1..1133
                     /locus_tag="VN039_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45921.1"
                     /translation="RNEANYFSGSVRSPTALVLDIMLHRMALRVLPSLSLAVTEETVR
                     KQKRIVMFVPGLVAFGVYRLVKHLVPISDPLVLLAVSSLVAMVASVLAYRIGRSAPWS
                     VIAREDGARLLSWLAGWIGAVYGIQLSLLVLTLLWIMNYDYLQHPDGPAMMAIIISCT
                     AVARDAFEIGHVRKLSLSGRPFLTFPDGERLRALVQRRAGQLGPWVVVGLGMGAITSL
                     SGMIIADVQGSALAQLLSVTVLGGGLALCAYFGGLHPSMSWAHTLRQTAPSELLKYWW
                     WPGMAFASTYYLVTMGLLLFVVRQPTITPGSAAVMGALVTALMGVYGYYLGDRRHVED
                     EQAPQLSSGMLRCPFVMGILGKTVRAEGTAGEMVLDKIGTKG"
     gene            1157..3013
                     /locus_tag="VN039_07890"
     CDS             1157..3013
                     /locus_tag="VN039_07890"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWV45922.1"
                     /translation="MKRSAISIAICIAMVAIAIGCGWNGPDLSFAESSTDVYFGTEGT
                     PQGPAAPTPHETVYPRVGSLDSRLLVWFVTQQHTYFGGFVLALPLFCALLEFLGLITH
                     KPALALRYDGLARDLAKVAVLALSVTALIGSVMLTMFITLYPSFMTYMGGTFKGFMPV
                     YAAVFLGETLLLIVYYYGWNRMTDRGMKWIHAAIGILTNIVGTLLLVMVNAWSAFMMS
                     PAGVDAQGRFLGNAWHLLHSALWNPLNVHRFLADIMSGGAVVLAYACYRFFTSKTDEE
                     RAYFDWVGYVFLFVTVCALIPMPIAGYWLMKSVFMFRQTMGVTMMGGLLTWLFVVQAL
                     LIGVLFLGINYYLWQSMARIKGGERYQPYYQLLLGVLMLALFIWLTPHTLLTSASEVK
                     AMGGAQHPVIGNYGVMSAKNGAVNILILVTALSFLYYRRANRTMTISWVKTGNTLITV
                     LYVVGALNIVWLSVYGFYLPAKVRVGLSFPQAVTTLTVIVVGVVINRLMLRGAVVRGP
                     IQWGRIPLRGMVGLFGLAASFSWVMGLMGYIRSSGRLSWHVSELMADVSPWAFTPDLQ
                     FATKMVTLNMVVFWAAVLALFWMCEHGQQPVMGEEFRDKQAPLLTPVPSQET"
BASE COUNT          562 a          781 c          996 g          840 t
ORIGIN      
        1 gaagaaatga agcgaattat ttttccggct cagtccgcag tcctactgcc ttagtcctgg
       61 acattatgtt acatcggatg gccttgcggg ttcttccgag tttaagtttg gcagttacgg
      121 aagagaccgt gcgcaagcag aaacggattg tgatgtttgt ccccggactg gtcgcgttcg
      181 gggtgtatcg tctcgtcaag catctcgttc cgatctcaga cccccttgtc ttgttggcgg
      241 tcagtagtct ggtggcaatg gtcgcctcgg tcctggccta tcgaattggc cggtctgccc
      301 catggtccgt cattgcccgc gaagatgggg cgcgtctcct gagctggctt gccggatgga
      361 tcggagccgt gtacggcatt cagctctcgt tgttggtact gaccctgtta tggatcatga
      421 attacgatta tctccaacat cctgatgggc cggcgatgat ggcgatcatc atctcatgca
      481 cggctgtcgc ccgcgatgcg tttgagatcg gacatgtgcg taagctctcg ttgtcgggac
      541 ggccatttct cacgttccct gatggcgagc gacttcgtgc cttggtgcag cgtagggctg
      601 ggcaattggg accttgggtc gtggtgggcc ttggtatggg tgcgattaca tcgttgtcgg
      661 ggatgatcat cgcggatgtt caaggatccg cgttggctca attattgagt gtgacggtcc
      721 ttggcggtgg cctggccctc tgtgcgtatt ttggcggact ccatccatct atgtcatggg
      781 cccatacgct ccgtcagacc gcgccgagtg agttgttgaa atattggtgg tggccgggaa
      841 tggcctttgc ctcgacctat tatctcgtga cgatgggact gttgctgttt gtcgtgaggc
      901 aaccgacgat tacgccgggc tcagccgccg tgatgggcgc gctggtgacg gcgctgatgg
      961 gtgtgtacgg atactatctg ggcgatcgcc gtcatgtgga agatgagcag gccccacagc
     1021 tatcgagtgg aatgttacgt tgcccgtttg tcatggggat tctcggaaag acggtcaggg
     1081 ctgagggtac ggccggggaa atggtgctcg acaagattgg aacgaagggg tagatgttat
     1141 gggtcaagga ttcaggttga agcgttccgc gatcagtatt gcgatctgca tcgcgatggt
     1201 cgcgatcgcc attggatgcg gatggaatgg accagatctc tcatttgcag aatcatcgac
     1261 cgatgtgtat ttcggcactg aggggactcc gcagggaccg gcggctccca cccctcatga
     1321 aacggtctat ccgcgcgtcg ggtcgttaga cagtcggctg ctggtgtggt ttgtgacgca
     1381 gcaacatacc tattttggag gattcgtcct cgccctgccc ttgttttgcg ccttgctcga
     1441 gttccttggg ttgatcaccc acaagccggc gttggcgctt cgctacgatg gtctggcgag
     1501 agacctcgcg aaggtggcgg ttttggcgct gtcggtcacg gcactcatcg gcagcgtgat
     1561 gctgacgatg ttcatcacgc tgtaccccag tttcatgacc tatatgggcg ggacgtttaa
     1621 gggcttcatg ccggtctacg ccgccgtgtt cctcggagag acgctgctgc tgatcgtcta
     1681 ttactacggt tggaatcgga tgacggaccg gggcatgaag tggatccacg ccgcgatcgg
     1741 cattctgacg aacattgtgg ggaccctgtt attggtgatg gtgaatgcct ggtcggcctt
     1801 catgatgtca cctgccggag tggatgcgca gggacgcttc ttggggaatg cctggcatct
     1861 gctgcactct gcgctctgga accctctgaa cgtccatcgt ttcctcgcag atatcatgtc
     1921 cggtggggcg gtggtgctgg cctatgcctg ttatcgattt tttacgagca agaccgatga
     1981 ggagcgagcc tatttcgatt gggtcggata tgtcttcttg tttgtcacgg tctgcgcgct
     2041 cataccgatg cccattgccg gctactggct gatgaagtcg gtgttcatgt ttcgccagac
     2101 tatgggcgtg acgatgatgg gtgggttgct cacgtggctg tttgtggtcc aggcactctt
     2161 gatcggtgtc ttattcctgg ggatcaacta ctacctctgg cagagtatgg ctcgaatcaa
     2221 agggggcgaa cgctatcagc cctactatca gctcttgttg ggtgtgttaa tgctggcact
     2281 ctttatttgg ctgacgccgc atactttgtt gacgtctgcc agtgaagtaa aggcgatggg
     2341 aggcgctcag catcccgtga tcggcaacta cggagtcatg tcggccaaga atggtgccgt
     2401 caatatcttg attctcgtca cggctctcag ctttttgtac tatcgccgag cgaatcgaac
     2461 catgaccatc tcatgggtca agactggaaa cacgctcatc acggtactgt atgtggtggg
     2521 tgcattgaat atcgtctggc tgtcggtcta cgggttttat ttgccggcaa aggttcgggt
     2581 cggcctttca ttccctcaag ccgttacgac gctcacagtg atcgtggtcg gtgtagtgat
     2641 caatcgcctc atgcttaggg gagcggtggt gcgtggccca attcagtggg ggagaattcc
     2701 gctgcgcggc atggtcggat tgttcgggtt agcggcgtcc tttagctggg tgatgggctt
     2761 gatggggtac attcgttcgt cgggacgctt gtcttggcat gtcagtgaac tgatggccga
     2821 cgtctcacca tgggccttca ccccggacct ccaatttgcc acgaaaatgg tgacgttgaa
     2881 catggtggta ttttgggcgg ctgtcctcgc gctgttctgg atgtgcgagc acgggcagca
     2941 accggtgatg ggtgaggaat tccgcgacaa acaagctccc ttgctcacgc cggttccgtc
     3001 gcaagaaaca tagaaaggga atgacggaca tgaagcgtcg ggttctgcgt tatgcagcaa
     3061 tcgttctgag cctgcctatg ggcattgtta tgtggcaacc atccgtgttt gcccaagggc
     3121 aaccattggt cctggaagac tttcaagcga tggaagcgga tggatttcct tcgcagtgg
//
LOCUS       DATMTI010000490         1941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_442822, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000490 DATMTI010000000
VERSION     DATMTI010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442822"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="VN039_07895"
     CDS             <1..1537
                     /locus_tag="VN039_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HWV45923.1"
                     /translation="LKTYRLQGGHADLLRVGSRGATERVRPYQARSLRDRYRVRFENG
                     LKARVSHAASAAGISRQFVHDVVNVDLKLGSLQRALQLAGAASTADTARDERRRSATR
                     KRTLTRAFAREAAQLLNRELQEDDLAVASLVEEAYSAVLLAHPKVPRGDLAKVRQLVS
                     LANEWKETLSSGHRNFDEFLAKTRRVVAGTCVGLGQSQIRLEQGTFDWVIVDEAARCT
                     SGELAVPLQLGSRVVLVGDQRQLRPMVERAVQKALQDEFSAAGKALAQSDFERAFQSA
                     YGRRNAQVLDEQYRMMPVISDLVSDTFYGPHGVKLKPSADRKADPAFADLKQDLATPV
                     VWFDTSQMAAAKERSGNDGRDIWNDAEIATVISILHRLSKEETLVGAFAKRAEPAIGV
                     ICMYSEQKRRLEKEWSQQPFAEGFRRSVVIDTVDAYQGKENEIVIVTLVRSNELKTPG
                     HVGRENRCNVAISRAKERLYIVGDTTMWSSPKCHSPMSTVLSRLRDMEKGTGEVRPAG
                     DIQ"
     gene            1534..>1941
                     /locus_tag="VN039_07900"
     CDS             1534..>1941
                     /locus_tag="VN039_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45924.1"
                     /translation="MSASSERIEIDEVDFQISCRRFTIRATITRDRQLPVVDEFVLRL
                     LSVLDQMPVARMRAWFGFTESEMQTVLVDMGRRSLVELTGDDLRLAAAGRELFRSISR
                     DGVPHIVEVAPLLESVWFDLVSRNMVPRSRARPT"
BASE COUNT          435 a          519 c          566 g          421 t
ORIGIN      
        1 tttgaagacg tatcgattac agggcggcca tgccgacttg cttagggttg gatcaagggg
       61 agcaactgaa cgtgtccggc cttaccaagc gcgttcattg agggaccggt accgagtgcg
      121 gtttgaaaac ggcctgaagg cacgcgtctc ccacgccgct tcagccgctg gcatctcaag
      181 gcaattcgtg cacgacgtag tcaatgtcga tttgaaacta gggtcacttc agcgcgcact
      241 ccagctggcg ggagccgcat ccaccgcgga caccgcccgc gacgaacggc ggcggtctgc
      301 cacccgcaag aggacactca cacgagcatt cgctcgcgag gccgcgcagc ttcttaacag
      361 ggagctgcag gaggacgatt tggccgtcgc ttctctagtg gaagaggctt attccgcagt
      421 tctcttggct catccaaaag tgccgcgtgg cgatcttgcg aaggtgcggc agttagtgtc
      481 ccttgcaaat gagtggaagg agacgctgtc ttccgggcat cggaatttcg atgagtttct
      541 ggcaaagact agacgtgtgg ttgccggcac atgcgttggc ctcggacaat cccaaattcg
      601 tctggagcaa ggaacgtttg actgggtcat agtcgatgag gccgcaagat gcacaagcgg
      661 cgagctagcg gtacccctgc aactcggcag cagggtggtg ctggtcggag accagcgaca
      721 gcttcgcccc atggttgagc gagcggtgca aaaagcactt caagacgagt ttagtgctgc
      781 tggaaaagcg cttgcgcagt ctgacttcga gcgcgcattc cagtccgctt acggtcgtcg
      841 caacgcccag gttctagatg agcagtatcg aatgatgccc gtcattagtg atctcgtttc
      901 ggacacattt tacggtcctc atggcgtcaa gttaaagccg tctgcagacc gcaaggctga
      961 tcccgctttc gcagacctaa agcaggacct cgctacgccg gtagtatggt ttgacacgtc
     1021 tcaaatggca gctgccaaag agcgttccgg taatgacggc cgggacatct ggaatgatgc
     1081 ggagattgcg acggttatat cgatcctcca ccgtctttcc aaagaagaga ccctggtcgg
     1141 tgctttcgcc aagagagccg aacccgctat cggtgtcatc tgcatgtaca gcgagcagaa
     1201 acggcgtctt gagaaagaat ggtcccagca accatttgct gagggctttc gtcgctccgt
     1261 agttattgac accgtagacg cctatcaagg gaaagaaaac gagatcgtga tcgtcacttt
     1321 ggttcgctca aacgagctta agacacctgg tcacgtcggg cgcgagaaca gatgcaatgt
     1381 tgccatttca cgagcgaagg aacgcctcta catagtgggc gacactacga tgtggtccag
     1441 ccccaagtgt cactcgccta tgagcacggt actttctcgg ctgagggaca tggaaaaggg
     1501 caccggagag gttcgaccag cgggagatat acaatgagtg catcatccga aagaatcgaa
     1561 atcgacgaag tcgactttca gatttcctgt cgtcgtttca cgatccgcgc caccataacg
     1621 cgtgaccgtc agcttcctgt agtagatgag ttcgtgcttc ggttgctctc agtcctcgat
     1681 caaatgcccg tcgcgaggat gcgggcatgg ttcggcttta cagaaagcga gatgcagacg
     1741 gttctcgtgg acatgggaag gcgcagccta gtcgaattaa ccggcgacga tcttcgattg
     1801 gccgccgcag gacgagagct gtttcgatcg ataagccgtg acggtgttcc tcatattgtg
     1861 gaggtcgcac cccttctcga aagtgtttgg ttcgacctcg ttagtcgtaa catggttcca
     1921 cgttcccgcg cacgacctac t
//
LOCUS       DATMTI010000491         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_358744, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000491 DATMTI010000000
VERSION     DATMTI010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358744"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VN039_07905"
     CDS             <1..581
                     /locus_tag="VN039_07905"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV45925.1"
                     /translation="YCPVFGVVGIDMSGNDQSGRSSDTGRLGRRKVHTDRATKIQRAI
                     EINRPPNEIYRFWRSLDNLPRIMNHLDSVQVINDRLSHWVVKAMPGVPQVEWDAEIIN
                     DVENRRIGWRSLEGADVDNAGSVRFKATGGGRRTWLTVTLQYKSPGGELGDAVAKWFG
                     EDPNTTIAKDLQRFKEEMETGVFSQAGAEGEA"
     gene            587..1132
                     /locus_tag="VN039_07910"
     CDS             587..1132
                     /locus_tag="VN039_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWV45926.1"
                     /translation="MPRKARSRCDPYDLIVLPDDHIVGSGTASLTVITYCDFECPYCR
                     QACFLIGGLRTRFTDRVRFVYRHFPLIKKHPHAQLAAEASEAAALQDRFWPMHDLLFQ
                     NQAHLEIDDLYVYAAMAGLDVPHFKDDLHAGAGVLKIRRDVLSGHRCGVQGTPTFFVN
                     AVLVSDSDELERTVLLQVKKQ"
     gene            1437..1994
                     /locus_tag="VN039_07915"
     CDS             1437..1994
                     /locus_tag="VN039_07915"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWV45927.1"
                     /translation="MRRTLFVGLFMTVTLSFFTGMVGAQQSVQSDPKGEQLDPKADTQ
                     TFLTIAAEEQQVAIALGQLAAQRAKNEKVKEFGSNMVEAHKKVRREAEQLASKHQVPL
                     SAKLTLEDKKKVDELSQMSGHEFDRAYMDFTLHNHETTLEEYQQHISTMRYPDLREWF
                     TSTLPVLKAHRDQARLVKNSLQTSP"
BASE COUNT          530 a          518 c          609 g          457 t
ORIGIN      
        1 gctattgccc tgtgttcggg gtcgtgggta tcgatatgag cggcaacgac cagtcgggtc
       61 gatcaagcga caccggtcga ttgggccggc gcaaggttca tacagaccgc gcaacgaaaa
      121 ttcaaagggc gatcgagatc aatcgtccac cgaatgaaat ttatcgcttt tggcgttcat
      181 tggataattt gccccgtatc atgaaccatc ttgattcggt acaggtcatc aatgatcgac
      241 tgtcccactg ggtggtgaag gcgatgcccg gtgtccctca ggtagagtgg gatgccgaaa
      301 tcatcaacga tgtggagaat cgacgcatcg gatggaggtc gctggaaggc gccgatgtgg
      361 acaatgccgg ttcggtgagg ttcaaggcga cgggtggcgg acggcggact tggttgaccg
      421 tgacgctgca gtataaatca ccgggcggtg agctcggcga cgctgtggcg aaatggttcg
      481 gggaagatcc gaatacgaca attgctaaag atctgcagcg attcaaagaa gagatggaga
      541 cgggagtctt ctctcaggcc ggcgctgagg gggaagcgta ggtattatgc ccaggaaggc
      601 tcgcagcagg tgtgatccat atgacctcat cgtattgcct gacgatcata tagtggggag
      661 cgggacggcc tcgcttaccg ttattaccta ctgcgatttc gaatgtccct actgccgaca
      721 agcatgcttt ctcattggtg ggttgcgcac tcgattcacc gaccgggtgc ggtttgtgta
      781 ccggcatttc cccttgatca agaaacatcc gcatgcacag ctcgcagcgg aagcctctga
      841 agccgccgct ctccaagacc gcttctggcc catgcatgat ttgctgttcc aaaaccaagc
      901 gcatctcgaa atagatgatc tctatgtcta tgccgcgatg gccggattgg atgtcccaca
      961 ctttaaagat gatctacatg cgggagccgg ggtattgaaa atacggcgcg atgtgttgtc
     1021 cggccatcgg tgtggagtgc aggggacgcc cacattcttc gtgaatgccg tgttggtgtc
     1081 ggacagtgat gaactggagc gaacggtatt gctgcaagta aagaagcaat aattgtaggc
     1141 cagatcagaa tggtggaaaa actcagttga tgccctaaag aaggtctgtc cgctacggga
     1201 catcgtgtcc gttgaaagca ttcaaggcga tcacgtaccg gccggattca tttgatgagc
     1261 gattggcgtg accgagccac ctaccagtaa aattacgagg cttcaacaca tggctccgac
     1321 ggttcaggtt aagccgaact caggcccatg ttttgcatca gaggggaatg cccaggtact
     1381 gggccggagg aaatagatct aaatcacagc tactcaccct atccaaggag catcccatgc
     1441 gtcgcacgct attcgtaggt cttttcatga ccgtcacgct gagtttcttt acaggaatgg
     1501 ttggagcgca acagagtgtg caatcagatc ccaaaggaga acaattagat cctaaagcgg
     1561 atacgcaaac gttcctcaca atcgccgccg aagagcagca agtcgcgatc gctctcggtc
     1621 aacttgcagc acaacgagcg aagaatgaga aggtaaagga attcggctca aatatggttg
     1681 aagcgcataa gaaagtccgc cgggaagcgg aacagctggc atcgaaacac caggtgccgt
     1741 tgtctgcgaa actcactcta gaggataaga aaaaagtaga cgagctgtcg caaatgtccg
     1801 ggcatgagtt cgatcgcgcc tacatggatt tcactctgca caatcatgag acgacgttgg
     1861 aggaatacca gcagcatatc agtacaatgc gatatccgga ccttcgggaa tggtttacct
     1921 ccaccttgcc ggtcctgaag gcccatcgtg atcaggcacg gctggtcaaa aactctttgc
     1981 aaacgagtcc gtgactcgtc aagggtgtga taagaattac cgctaaacaa gacggctact
     2041 attccgagct gccgggggcg actcccttga gaatggcgct tcggatcagc gcaacggggg
     2101 tcgcactgcg ctac
//
LOCUS       DATMTI010000492         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_442854, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000492 DATMTI010000000
VERSION     DATMTI010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442854"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VN039_07920"
     CDS             <1..249
                     /locus_tag="VN039_07920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWV45928.1"
                     /translation="FYSTKSHKYLALKVTAVNEHSPSLMPVWPAADWHERETYDLLGI
                     HYDGHPNLTRILMPTDWLGHPLRKDYKVNDPRLVWNER"
     gene            264..1514
                     /locus_tag="VN039_07925"
     CDS             264..1514
                     /locus_tag="VN039_07925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWV45929.1"
                     /translation="MSIKDIPGVFTEQGLKTEDLVVNIGPQHPSTHGVLRLITTLNGE
                     VVKDMVPVIGYLHRSKEKMAESRSYFQYQPTLDRVEYLSSFFDHYVYVSAIEKIANLK
                     VPKRVQYIRMITMELNRITSHLLWFGTFLLDLGATSPLFYAFREREEIFKLFEDVTGA
                     RMMYNYYRFGGVNNDLPPGWIEKCRQFIRTMPQKINEYESIVTKNPIVLDRTVGIGHL
                     DYETCMKYGVTGASLRANGYDQDLRKTNPYCCYDEVQFDVPLGKHGDVYDRYLVRMAE
                     MRESCKIIEQCLDKIPGGDTQNIRNKQAEVEAAKAAAKERGEDPKSVPDIDPDWLTQG
                     QKINLMTYKPPAGEVFSACESPRGMLGCYMISDGTPKAYRVKWRGASFSNLSVLPEMM
                     IGSLYPDLMAIFGSLDVILPEVDR"
     gene            1515..2819
                     /gene="nuoH"
                     /locus_tag="VN039_07930"
     CDS             1515..2819
                     /gene="nuoH"
                     /locus_tag="VN039_07930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWV45930.1"
                     /translation="MNQPVFDFPNWLPHLLEQLVGRFGVSINWVDLAPWLTVLWAIVV
                     FTLIAVVAQASVIFMVLMERKVLAWMTQRKGPNRVGPWGALQTLADGVKLLFKEDIIP
                     KGANKFLFTIAPALFFLPTFVFFGLIPFTNELLAVALPAGALFVFAISSLSVVGLVLA
                     GWASNNKYSLLGGMRSAAQAISYEIPLVMAVLGVVLFTGSMNIADIVKAQAGPLWNWY
                     GVLLTPVSLAIFFISTLAEVNRVPFDLPEAESELVSGYNTEYTGMKFALFFLAEYAAL
                     FAMSSFTVAFFFGGYFSPFGSFILELATHTAADKMTGVMQLGLQVEMLFWFMMKTYFF
                     IFLAMWVRATLPRLKPDQLMGFSWKFLIPLSLINIFVIAIEKYMVLPNGKPVQTALPV
                     LSPTTVAPWAIWLVIAVVVFGGFFVLMSRLLSEKLEARLARR"
     gene            2816..3316
                     /locus_tag="VN039_07935"
     CDS             2816..3316
                     /locus_tag="VN039_07935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWV45931.1"
                     /translation="MSTKHDDIKENRVIEGLKAIGEGMATVLNHYFRPPVTLEYPEVM
                     PDLSDRFHGRLCLLTKSDGTDLCIGCQACARVCPCNDLIQIEMHRDANKKPVIDMFTI
                     DMGRCIFCGNCTEVCPVDCIKMLPDFELSDYSREALVLDKKDLTLSGEEADHWRLVRG
                     MEPLVE"
BASE COUNT          693 a          918 c          906 g          818 t
ORIGIN      
        1 ttctattcca ccaaatccca caagtacctg gccctcaaag tgacggctgt caacgagcat
       61 tcgcctagcc tgatgccagt gtggcccgcc gctgactggc acgagcgtga aacctacgac
      121 ctgctcggga ttcactacga tggccacccc aacctcaccc gtattttgat gccgactgac
      181 tggttaggac atccgctgcg taaggattac aaagtgaatg atcctcgttt ggtctggaat
      241 gaacgttaag gacggcagaa ccaatgagta ttaaagatat tccgggcgtt tttaccgagc
      301 aaggtctcaa aaccgaagac ttggtggtca acattggtcc ccagcacccg agtacccacg
      361 gcgtattgcg tttaatcacc accctgaacg gcgaagtcgt gaaggatatg gttccggtga
      421 tcggctactt gcaccgcagc aaggaaaaaa tggccgagtc ccgttcctac ttccagtacc
      481 agcccacctt ggaccgggtt gagtacctct cttccttctt tgaccactac gtgtacgttt
      541 ccgccatcga aaagatcgcc aacctgaaag tgcccaagcg cgttcagtac attcgaatga
      601 tcacgatgga gctgaaccgg attacctccc acttgctgtg gtttggtacg ttcctcctgg
      661 acttgggcgc taccagcccc ctgttctacg ccttccgcga gcgtgaagag attttcaagc
      721 tgtttgaaga tgtcactggc gcgcggatga tgtacaacta ctaccgtttt ggcggcgtga
      781 acaacgatct gccgcccggc tggattgaaa agtgccgtca gttcatccgt accatgccac
      841 agaagattaa tgagtatgaa tccattgtga cgaagaaccc catcgtcctg gatcgaactg
      901 tgggcatcgg ccatctggat tatgaaacct gcatgaagta cggtgtgacc ggcgcgagcc
      961 ttcgtgctaa tggctacgac caggatctcc gaaagaccaa cccgtactgc tgctacgatg
     1021 aagtgcaatt cgacgtgccg ctgggcaagc atggcgatgt ctacgatcgt tacctggttc
     1081 gtatggccga aatgcgcgaa tcctgcaaga tcatcgagca gtgcctggac aaaatccctg
     1141 gcggggatac ccagaatatc cgcaacaagc aggctgaagt ggaagcggcc aaggctgcgg
     1201 ccaaagagcg cggtgaagat cccaaatccg tgccggatat cgatcctgac tggctgacgc
     1261 aaggtcaaaa gattaacctg atgacctaca agccaccggc aggtgaagtg ttcagcgctt
     1321 gcgaatcccc tcgcggaatg ctgggttgct acatgatcag tgatggaacg cccaaggctt
     1381 accgtgtaaa gtggcgtggt gcctccttct ctaacctgtc cgtcctgccc gagatgatga
     1441 ttggcagtct ctatcctgac ttgatggcta ttttcggtag cctggatgta attttgccgg
     1501 aggttgatcg ctaaatgaac caacccgtct ttgatttccc caattggctt ccccacctgc
     1561 tggagcagtt ggttggccgt ttcggcgtct ccatcaactg ggttgatctt gctccctggc
     1621 tgaccgtact gtgggcgatc gtcgtcttta ccttgattgc cgtcgtggct caggcctcgg
     1681 ttatctttat ggtcttgatg gaacgtaaag tgctggcctg gatgacccaa cggaaaggtc
     1741 ccaaccgggt tggcccttgg ggtgccctgc aaacgctggc ggatggcgtg aagttgctct
     1801 ttaaagagga catcatcccg aaaggcgcca acaaattcct gttcacgatt gccccggcgc
     1861 tcttcttcct gccgactttc gtgttctttg gcctgattcc ctttaccaat gaactgctgg
     1921 ctgtggcgtt gcctgccggt gccctgtttg tcttcgccat ttcctccctc tcggtggtgg
     1981 gcctggtgct ggccggttgg gcgtccaaca acaaatactc cctgttgggc gggatgcgta
     2041 gcgcggcgca ggccatcagc tacgagatcc ccctggtgat ggccgtgttg ggtgtggtgc
     2101 tgttcaccgg ctccatgaac attgccgata tcgtaaaggc ccaggccggt cctctgtgga
     2161 actggtacgg cgtcctgttg accccggtca gtctggcgat cttctttatt tccaccctgg
     2221 cggaagtcaa ccgcgtgcct ttcgacttgc cggaagcgga aagcgaattg gtcagcggtt
     2281 acaacacgga atacaccggt atgaagttcg ccttgttctt cctggcggaa tacgcggccc
     2341 tgtttgcgat gagttccttt acggtggcct tcttcttcgg cggctacttc agcccgttcg
     2401 gttcgtttat tctggaactg gcgacccaca ccgccgccga taaaatgacc ggcgtgatgc
     2461 agttgggtct gcaagtggaa atgctcttct ggtttatgat gaagacctac ttctttatct
     2521 tcctggccat gtgggttcgg gcgaccctgc cccgtctcaa gccggaccag ttgatgggtt
     2581 tcagctggaa gttcctgatt cccctctccc tcatcaatat ttttgtgatt gccattgaga
     2641 agtacatggt gctgccgaac ggtaagccgg tgcaaaccgc cctgcccgtt ctgtctccca
     2701 cgactgtagc tccttgggca atctggttag tgattgcagt ggtcgtcttc ggtggtttct
     2761 ttgtcctgat gagccgactg ctgagcgaaa aactggaagc gaggttagcg cgccgatgag
     2821 caccaaacac gatgacatta aagaaaaccg ggtcattgaa ggcctgaagg caatcggcga
     2881 aggcatggct accgtgctga accactactt ccgcccgccg gttaccctgg aataccctga
     2941 agtaatgccc gatctcagcg atcggttcca tggacgcctg tgtctgttga ccaagtccga
     3001 tggcacggat ctctgcatcg gctgccaggc ctgcgcgcgg gtttgtccct gcaacgacct
     3061 gattcagatc gaaatgcacc gggatgccaa caagaagccg gtcatcgata tgttcaccat
     3121 cgatatgggg cgttgcatct tctgcggcaa ctgcaccgag gtctgcccgg tggactgcat
     3181 caagatgttg cctgactttg agttgtcaga ctactcccgt gaagctctgg tactggacaa
     3241 aaaggacttg accctttcgg gggaagaggc cgatcactgg cgtctggtcc gtggtatgga
     3301 acccctggtt gaataatgaa tcatttataa accgg
//
LOCUS       DATMTI010000493         1683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_358839, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000493 DATMTI010000000
VERSION     DATMTI010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358839"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VN039_07940"
     CDS             <1..1386
                     /locus_tag="VN039_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWV45932.1"
                     /translation="DSRGAGPAFTELNVYVRTTRLGAEDLKELRARNLKPIPNGLQIL
                     VSGTTGHVVEPVLLFMREKCIRRLLPGLPNELKKSPNTRKTIAYDLKDFFDFLDAESL
                     TIDAIDDAVLNSYVSSMRTNLSPVTGCPYKDNTVIRRLSTTKMFCAWAQDRGLLRHRF
                     SIKKVESKRPTDRKFLPHLQTHSTRENFAAEVDAPRTPDLGEGVTTLSPEEVQGILDA
                     LGPTVPIASLGEPEGSFEGGSVRDRLMASCGLYVGLRRAEVCDLELSAVNAVHITDET
                     SPTTMQLIRVHGKGDKWRNVNVPTWLILAMRYYIATERADVIAKARAKDPGYVPPNRV
                     FLNRPNARTGLGAEVAPKTLGQVFHDAQLRRNSSCLSQAPTAPGVSKRYGYHVLRHTY
                     AVWTYICRKHDGDTDPIKYIQAQLGHAHYSTTADTYLKYSASAETRLFDAFFGARKAM
                     MADRFGASEHG"
     gene            1379..>1683
                     /locus_tag="VN039_07945"
     CDS             1379..>1683
                     /locus_tag="VN039_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45933.1"
                     /translation="MAKSGAAKTRHKHQDTTAARPVGDDGSRRQFDEAILYNEATRAL
                     DVRLVGQKGNISITVDCSSWLVAPGLAFVFARASLEACKGLMLRTKEAHFDCLTYGI"
BASE COUNT          392 a          487 c          476 g          328 t
ORIGIN      
        1 gattcaagag gggcggggcc ggccttcacg gaactgaatg tgtacgtgcg caccacgcgc
       61 ctcggggcgg aggatctaaa ggaactcagg gcgcgcaacc tcaagcccat ccccaatggc
      121 ttgcagatcc tcgtctcggg aacgaccggc catgtcgtgg agccggttct cctcttcatg
      181 cgcgaaaaat gcattcgacg actgctgcca ggtctgccga acgaactgaa gaaaagccct
      241 aacacccgga agacaatcgc atacgacctg aaggacttct tcgactttct ggatgcggag
      301 agcctaacta tcgacgctat cgacgatgcg gtgctgaaca gctatgtctc atcaatgcga
      361 accaatttgt cgccggtaac tggctgcccg tacaaagata acaccgtcat tcggcgcctc
      421 tcgaccacga agatgttctg cgcgtgggcc caggataggg ggctactccg tcatcggttc
      481 agtatcaaga aagtggaaag caagcgaccc accgacagaa aattccttcc gcaccttcag
      541 actcattcaa cgagggaaaa cttcgccgcc gaggtcgacg cacctcgaac gcctgatttg
      601 ggtgagggcg tcacaacgct ttcgccggaa gaggttcagg gaattctcga tgcgcttggc
      661 ccgaccgtac cgatcgcgag cctgggagaa cccgaagggt cgttcgaagg cggctcagtc
      721 cgcgaccgac tcatggcgtc atgcggactc tacgtcggat tgcgtcgcgc ggaagtctgc
      781 gatcttgaac tgagcgcggt caacgcggtc catatcaccg acgagacgtc ccccacaaca
      841 atgcagctga tccgtgttca cggcaagggc gacaagtggc gcaatgtcaa cgtgccaaca
      901 tggctgatat tggcaatgcg ctattacatt gcaactgagc gagctgacgt tatcgcaaag
      961 gcgcgagcga aggatcccgg ctacgtcccg cccaatcgag tgttcttgaa tcggccgaac
     1021 gcacgaactg gacttggcgc agaggtcgca ccgaagacac tcggtcaagt atttcacgat
     1081 gcgcagcttc gacgaaactc aagttgcctc tctcaagctc ccaccgcgcc aggtgtcagc
     1141 aagcgctatg gatatcacgt gcttcggcat acctacgcgg tgtggactta catctgccgc
     1201 aagcacgacg gcgatactga tccaatcaag tacatccaag ctcagttggg tcatgcccac
     1261 tattcgacga ccgccgacac gtacctgaaa tactcggcat cagccgaaac aagacttttc
     1321 gacgcgttct tcggtgcgag gaaggcgatg atggcggaca ggttcggggc cagtgagcat
     1381 ggctaagagc ggcgccgcca aaactcgtca taagcaccaa gacaccactg ctgcgcgccc
     1441 agtcggcgac gatggatcac ggaggcaatt cgatgaagcc atcttgtaca acgaagcgac
     1501 tagggcgctg gacgttcggc tggtaggtca gaagggcaac atcagtatta cagtcgactg
     1561 tagttcgtgg ttggtcgccc ctggcttggc ttttgtgttt gcgcgggcat cactcgaggc
     1621 ctgcaagggt ctaatgctcc gtaccaagga ggcccacttt gactgtctca cctacggtat
     1681 ctt
//
LOCUS       DATMTI010000494         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_528547, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000494 DATMTI010000000
VERSION     DATMTI010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528547"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VN039_07950"
     CDS             <1..327
                     /locus_tag="VN039_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HWV45934.1"
                     /translation="EAVDALSFITHRERSYHRGRQMAEKMKELIPRQMFEIAIQAAIG
                     NKIVARETIGAMKKNVTAKCYGGDITRKRKLLEKQKEGKKRMKAVGSVEVPQEAFLAI
                     LRVGEE"
     gene            324..1094
                     /gene="lepB"
                     /locus_tag="VN039_07955"
     CDS             324..1094
                     /gene="lepB"
                     /locus_tag="VN039_07955"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWV45935.1"
                     /translation="MSLDQNQRNVDKLPGSPRGGEQASLPGAKLADNMDQSGRKSIVR
                     EYAEAIIVAMLLAFAIRVFVVQAFKIPSGSMIPTLLIGDHILVSKLSYGLQWPTDCQM
                     QWTFPPVNCYTSKTVVAFGKPQRGDIIVFRFPEDEEKDFIKRIVGLPGDTVQLRNKVV
                     LVNGRPLDDKAFTQRIDPGVIDGTVNPRDNFGPVTVPEGSYFVMGDNRDQSLDSRFWG
                     YVREEKIRGKAFRIYWSWNGQGNWTEWVRWERFAKAIQ"
     gene            1064..2131
                     /gene="rfaE1"
                     /locus_tag="VN039_07960"
     CDS             1064..2131
                     /gene="rfaE1"
                     /locus_tag="VN039_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HWV45936.1"
                     /translation="MGTIRQGDSVKKSSRASLPREESNGKSAVPSPKTLRQYLQRFPQ
                     ASVLVVGDLILDHYVMGRVSRISPEAPVPVVHVESESLRLGGAANVFNNILALGGKAD
                     LCGVIGSDESGRLLMKELGDKRSNRGGVVIDHDRPTTRKSRVIAHNQQIVRYDIEGRN
                     ELKAALRQKILRYVESRIRELSCVVVSDYAKGVVSPALMSEITRLAALRKVPIIVDPK
                     VEHFSYYKGVTVLTPNHLEAAQASGLHGDGDQTIDEAGAMIRQRLGCRSVLITRGEKG
                     MSLYEDDGASWHLPTKARQVYDVTGAGDTVIGTLALALSAGATIKTGAIMANYAAGIV
                     VGMVGTATVSPKQLSEAFGDV"
     gene            2124..>2715
                     /gene="kdsB"
                     /locus_tag="VN039_07965"
     CDS             2124..>2715
                     /gene="kdsB"
                     /locus_tag="VN039_07965"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HWV45937.1"
                     /translation="MSKTSRSVTVVIPARYGSSRFPGKPLVELNGKPMIQHVYERAVA
                     CRAVSDVLVATDDERIKQAVERFGGRVIMVTGEYRTGTDRVAAVARMFAGEYFLDLQG
                     DEIPLNPELLTDLIEPFLESGVSIGTLKRVMDPTEDLLNPAVVKVVTDARGDALYFSR
                     APIPFVRDDPGKQVIGGLHYIHLGLYLYKKETLLRFA"
BASE COUNT          610 a          702 c          844 g          559 t
ORIGIN      
        1 gaggcggtgg atgccctgtc tttcattacg catcgcgagc gttcgtacca tcgcggtcgc
       61 caaatggctg agaagatgaa agagttgatc ccccggcaga tgtttgaaat cgccattcaa
      121 gcggcgatcg ggaacaagat cgttgctcgg gaaaccatcg gggcgatgaa aaagaacgtg
      181 acggccaagt gttacggcgg cgacatcacg cggaaacgga agttgctgga aaaacaaaaa
      241 gaaggaaaga aacggatgaa ggcggtcggc agtgtcgaag ttccacagga agcgttcttg
      301 gccatcttga gagtcggaga agaatgagcc tcgatcaaaa tcagagaaat gtggacaagc
      361 tgcccggttc tccgcgggga ggagagcagg cctcgcttcc gggagccaag cttgcggaca
      421 acatggacca atcgggacgg aaatccatcg tccgggagta tgcggaagcg atcatcgtgg
      481 cgatgctgct agcattcgcc attcgcgtgt tcgtcgtgca ggcatttaag attccatcag
      541 gctcgatgat tcccaccttg ctgatcggcg atcacatttt ggtcagcaaa ctttcctatg
      601 gtcttcagtg gccgacggat tgccagatgc aatggacgtt tcccccggtc aattgctaca
      661 cctctaagac ggtggtggca ttcggcaagc cgcagcgggg cgacattatc gtgtttcggt
      721 ttcccgagga tgaagaaaaa gatttcatca agcgcatcgt cggactgccg ggggataccg
      781 tgcagcttcg gaataaggtg gtgctcgtga acggccggcc gctcgacgac aaggctttta
      841 cacaacggat cgaccccggc gtgatcgacg gcacggtcaa tcctcgcgat aatttcggac
      901 cggtgaccgt ccccgaggga tcctacttcg tcatgggcga caatcgcgac cagagtctgg
      961 acagtcggtt ctggggatac gtgcgcgaag aaaaaatccg cgggaaggcg ttccgtatct
     1021 attggtcctg gaatggacaa ggaaactgga cggagtgggt tcggtgggaa cgattcgcca
     1081 aggcgattca gtaaagaaaa gcagccgcgc gtctcttccg agagaggagt cgaatgggaa
     1141 gagtgcggtt ccgtcgccca agactctccg gcagtatctt caacgatttc cacaagcatc
     1201 agtcctcgtc gtcggcgacc tcattctcga tcactatgtg atggggcgag tcagtcggat
     1261 ctctccggaa gcgccggttc cggtagtcca tgtcgagtcg gaatcgctcc ggctcggtgg
     1321 agcggccaac gtgttcaaca atatcttggc gctgggggga aaggccgatc tctgcggtgt
     1381 gatcggatcg gacgaaagcg gacgcctgct gatgaaggag ctgggcgaca aacggtcaaa
     1441 ccgcgggggt gtggtgatcg atcatgatcg cccgacgacc agaaaaagtc gggtcatcgc
     1501 gcataatcag caaattgtgc ggtatgacat cgagggacgg aacgaactga aagcagcgct
     1561 gcggcaaaag attctccggt atgtggagtc tcgaattcgt gagttgtcct gcgtggtcgt
     1621 ctcagactat gccaagggcg tcgtgtcccc ggcactcatg tccgaaataa cccgactcgc
     1681 tgccttgcgg aaggttccaa tcatcgtcga tcccaaggtt gaacatttca gctactacaa
     1741 aggtgtcacc gtgctcacac cgaatcatct tgaggcggct caggcctccg gtttacacgg
     1801 cgacggcgac cagacgatcg acgaagccgg agcgatgatt cggcagcgtc ttgggtgccg
     1861 gtccgtcctg attacgcgcg gtgagaaagg catgagcttg tacgaggacg acggcgcctc
     1921 gtggcatttg cccacgaagg ctcggcaggt ctacgacgtc accggggcag gagatacggt
     1981 cattggaacg ctggcgctgg ccctgtcggc gggggcaacc atcaagaccg gagcgatcat
     2041 ggccaactac gctgccggga tcgtagtggg aatggtcggc accgcaactg tctcgcccaa
     2101 acaattgtcc gaggcgtttg gagatgtcta aaacctcacg gtcagtcacc gttgtgattc
     2161 cagccagata tggctcgtca cggttcccgg gcaagccgct cgtcgaactg aacggcaagc
     2221 cgatgattca acatgtgtat gagcgggccg tggcctgccg tgccgtttcc gatgtcttgg
     2281 tcgcaaccga cgacgagcgt atcaaacaag ctgtcgagcg atttggtgga cgcgtgatca
     2341 tggtcaccgg tgagtatcgg accgggactg atcgggttgc cgccgtggcc cgtatgtttg
     2401 cgggggaata cttcttggac ttacagggcg acgaaattcc cttgaatccc gagttactga
     2461 ccgacctgat cgagccgttt cttgaaagcg gcgtaagtat agggacgttg aagcgggtga
     2521 tggaccctac cgaggatctt ctgaatcccg cagtggtcaa agtcgtcacc gatgctcggg
     2581 gcgacgctct ctatttttca cgagccccga tcccgttcgt tcgcgatgat ccgggaaagc
     2641 aggtgatcgg cgggctccac tatatccatc tgggattgta cctgtacaag aaggagacgt
     2701 tgcttcgatt cgcag
//
LOCUS       DATMTI010000495         9214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_272883, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000495 DATMTI010000000
VERSION     DATMTI010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272883"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1525)
                     /locus_tag="VN039_07970"
     CDS             complement(137..1525)
                     /locus_tag="VN039_07970"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV45938.1"
                     /translation="MVLRDGEPFLQLRDTGGNAISPEGNMMAAHECPRIVIVGAGFGG
                     LYAARAFNHAAVQLTVIDRQNYHLFQPLLYQVATAGLSPSDIAYPIRSVLRKQNNARV
                     VQAQILAIKPREHRVMMADGVIDYDYLIVATGAGHSYFGHSEWEKFAPGLKSVTDALA
                     IRRRILGAFEQAEREPDAARRRAWLTFVIVGGGSTGLELAGAVAELAFKVMVEDFRRI
                     DSREARVILLQAMQRVLPTYPEALSAEAERALRGLGVEVRTNTAVTAIQPGWVAVGEE
                     RIPTATVLWAAGVEPSPLAHSLGVPLDHAGRVLVEPDLSIPGHPEVFVIGDLAACVDQ
                     TGHPLPGLAAVAVQQGHYVARHILNLIQGRPGDPFRYVDRGTLATIGRGKAVADFRVF
                     RMSGFWAWVAWLAVHLFLLIGFRNRFLVLFKWTWALMTSQHGARIMIDGTEILPAAHT
                     GVEEGVAGTGVS"
     gene            complement(1777..4170)
                     /locus_tag="VN039_07975"
     CDS             complement(1777..4170)
                     /locus_tag="VN039_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWV45939.1"
                     /translation="MNPTLVKMAFGHPGIEPRWTHGGKDGVGTAYATSSRIWFTMWNG
                     IVTEVYYPTVDRPQLRDWQYLISDGISFVQEEKRHLTSSCERLSDYALGYRCTNADPA
                     GRYAIVKEIITAPHLPCVLQRTQLTGDDAFLSALHLYVLCAPHLEIGGWGNNGSVIEA
                     AGHKILMAEKEGTWLALMATVPFARVSCGYVGYSDGWTDLADNFHMDWEFDHAPDGNI
                     ALTGELDLEGRREFTLGLAFGRTQHQALTTVFQALSLPFHDHLTRYTDQWHRAAAHRH
                     PLERASSDEGRLYHGSVGVLLAHEDKSYPGAFIASLSIPWGDAKGDGDQGGYHLVWPR
                     DMVHSATALLAAGHRETPLRALIYLASAQHQDGGFPQNFWIDGDPYWRGIQLDEVACS
                     IVLAWRLKQHEALQGFDPYPMVLQAAAYLVRHGPVTQQERWEEVSGYSPSTLASNIAA
                     LIGAAGFSRDRGDDATATFLEEYADFLEAHLEAWTVTTEGSLVPGISRHYIRILPEAV
                     DNAHPEEDPNRGELSIANRPPEAQRVFAARDIVDAGFVELVRYGIRPPHDPLIVDSLA
                     VVDGLLKVNTPFGPCWHRYNHDGYGQRDDSGPFVGWGTGRAWPLLTGERGHYELAAGR
                     DATPFIRAMEGFAAPTGLLPEQVWDKSDRPRHYLSCGRPTGSAMPLVWAHAEYIKLLR
                     SVVDGNVFDLIPDVATRYLGARPDRRSLEVWKPNRQVRRVKRGETLRIQVPASFRLHW
                     SADEWRTVEETPSIVTRLKIEFVDIPIRPTQRAPIRFTFFWTATDQWEGHDYAVAIE"
     gene            complement(4167..4916)
                     /gene="pgl"
                     /locus_tag="VN039_07980"
     CDS             complement(4167..4916)
                     /gene="pgl"
                     /locus_tag="VN039_07980"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWV45940.1"
                     /translation="MKPDVRVFRDVNELSRQLAEATVRIINETVQTNGSVSLALSGGN
                     TPRTLYRILSSQFRDQIPWKHVHVFWGDERCVPLDDPHSNYRMAWETLLDAVPCPPEH
                     VHPMRTQLWAPDVAAWDYEKTLRNHFSKDWPRFNLVFLGLGEEGHTASLFPESPALAE
                     TKRWVVAVDAPVEPHLRLTLTLPALTHAANAYFLVTGSNKAQALPHILLGSPDPKHYP
                     ASGVRLAQGTVIWWLDREAAALVVNRGGDTA"
     gene            complement(4951..5184)
                     /locus_tag="VN039_07985"
     CDS             complement(4951..5184)
                     /locus_tag="VN039_07985"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45941.1"
                     /translation="MMNKNMPQSDAALLKQMDAYWRATNYLSVGQVYLLDNPLLKQPL
                     KLEHLSDDDLFGSSPQKWIPRLSDHETEVICGG"
     gene            complement(5196..6407)
                     /locus_tag="VN039_07990"
     CDS             complement(5196..6407)
                     /locus_tag="VN039_07990"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HWV45942.1"
                     /translation="MNQQEPSSFILTINGGSSSIKYALFRDADYSAPVARGMVDRIGL
                     PNSEMLVTDAGTQQPSRRAVRAPDHRTCVGVITEWLEQQVGIGRLCAVGHRVVHGGKT
                     YTRPERVTPELLTELRRLCHYSPEHLPAEISLIESFHRYDSTLPQVACFDTAFHRDMP
                     LVARLLPIPRRYEKLGVQRYGFHGLSYAFLMRELKRVGAPGEAEGRIILAHLGNGASM
                     AAVKDGQAMDTTMGFTPASGLPMSRRAGDLDPGLVSYLARTEGMTVERFHEMVNTESG
                     LLGLSEVSSDMRDLLKQEQSDPRAAEAVGLFCYQARKWIGALTAVLGGLDTLVFSAGI
                     GEHAPAIRARICAGLEFLGIVINKPANEANQPVISSEASRVRVHVIPTDEEREIAESM
                     ALLRSTGKMDR"
     gene            complement(6574..7950)
                     /gene="zwf"
                     /locus_tag="VN039_07995"
     CDS             complement(6574..7950)
                     /gene="zwf"
                     /locus_tag="VN039_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWV45943.1"
                     /translation="MNGPHSDALVFFGATGDLAYKKIFPSLQAMVKHGSLDVPVIGVA
                     KNGWTIEQLRARARDSLQQHGGVDPAAFDRLCGLLRYVDGDYNDPATYQLVRKELGSA
                     QRPAHYLAIPPMLFGSVVEQLADAGCTHGARVIVEKPFGHDLSSAKKLNEILLDSFDE
                     AAIFRIDHYLGKRPIHNMLFFRFTNAFLEPFWNRDHIESVQMTMAETFGVQGRGAFYD
                     QTGAIRDVVQNHLFQVLTLLAMEPPVRTDSESIRDEKLKVLRAIPSLDETALVRGQFR
                     GYRTEQGVAPDSQVETFVALRLAVNSWRWQGVPFYIRAGKCLPVTCTEVVIRLRRPPT
                     IFPSCSAPPNYFRFRVNPEVTLALGTIVMDREEKMIGQSVEMLASTRPTADELDVYER
                     LLGDAMTGDRTLFAREHYVEEAWRIVDPVLKQGTPVHEYEPNTWGPSHVTQSLSPPGG
                     WHNPIVTG"
     gene            complement(7961..8977)
                     /gene="gnd"
                     /locus_tag="VN039_08000"
     CDS             complement(7961..8977)
                     /gene="gnd"
                     /locus_tag="VN039_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWV45944.1"
                     /translation="MQLGMIGLGRMGANMVRRLLKGGHQCVVFDRSPNAVQELVKEHA
                     VGVASLPDLAKKLEKPRAIWLMVPAAGVDQAIAHLLPHLEAEDILIDGGNSYYVDDLR
                     RAKELMPKRIHYVDVGTSGGVWGLERGYCMMIGGEEPVVKHLDPLFARLAPGRDAAPR
                     LAGREKIDGTAEQGYLHCGPNGAGHFVKMVHNGIEYGLMAAYAEGLGVLRAANIGKRT
                     QTIDAETTPLRDPAHYQYDLNLPDIAEVWRRGSVIASWLLDLTASALLKNPALSEFTG
                     RVSDSGEGRWTIKAAIDEAVPVPVLTAALYERFSSRGESDFQDKLLSAMRYEFGGHVE
                     QSTK"
BASE COUNT         1875 a         2876 c         2614 g         1849 t
ORIGIN      
        1 cttgactcgg tcgctacatt cagcagccgt taacaaactc gttgcgcgaa aaatccttct
       61 gacgccgtcc tcacggagga gtcctgtcat tcttcatgga tgccacgact ctcttgcttt
      121 tctcaagaag agggagttag ctgacccctg tcccggcaac tccttcttcg acgcccgtgt
      181 gtgccgccgg caatatttcc gtgccgtcga tcatgatgcg cgcgccatgc tgcgacgtca
      241 tcaacgccca tgtccatttg aacaggacga gaaagcgatt gcgaaagccg atcagaagga
      301 aaagatgcac ggccaaccac gccacccacg cccagaaacc cgacatccga aaaacacgaa
      361 aatccgccac tgcctttcct cgtccgatcg tagccagcgt ccccctatcc acatagcgga
      421 atgggtcgcc gggccgccct tggatcaggt tcagaatatg gcgtgccacg tagtgacctt
      481 gctgaacggc cactgcagca agcccgggca atggatggcc ggtctggtcc acacacgcgg
      541 caagatcacc gatcacgaat acctccggat ggccggggat gctgagatcc ggttccacca
      601 acacccgacc ggcatgatcc agcggcacgc cgagcgagtg cgccaagggt gacggctcca
      661 cgccggcggc ccacagcacc gtggcggtcg ggattcgttc ttcgccgacg gcgacccagc
      721 caggttggat cgcggtcacc gcggtattcg tgcgaacctc tacgcccagc cctctcagcg
      781 cccgctcggc ttcggcggac aacgcttcag ggtacgtcgg caagacccgc tgcattgctt
      841 gcagtaagat cacgcgcgcc tctcgcgaat cgatgcgccg aaaatcctcc accatcactt
      901 tgaacgcgag ttcagcgacc gcgccggcca attccaggcc ggtcgagcct ccgccgacga
      961 tcacaaacgt gagccatgcc cgtcgccgtg cggcatccgg ttcccgttcg gcctgttcga
     1021 acgcccctaa gatacggcgt ctgatcgcca atgcatccgt cacactcttc agcccagggg
     1081 cgaacttctc ccactcggaa tgcccaaagt acgaatggcc cgcgccggtc gccacaatca
     1141 agtaatcgta gtcgatgacg ccatccgcca tcatcaccct gtgctcgcga ggcttgatcg
     1201 ctaagatctg cgcctggacc acccgagcgt tgttctgttt gcgtaacacg ctgcggatgg
     1261 gatatgcgat atcgctgggc gaaagccctg ccgtcgcgac ttgatacaat aacggctgaa
     1321 acaaatggta attttggcga tcgatcacgg tgagctgaac agccgcatgg ttgaaggctc
     1381 gcgcggcata caacccgccg aatcctgcgc cgacaatcac gatacggggg cattcatgag
     1441 cagccatcat gttcccttcc ggagaaatgg cgttcccacc cgtatcacgt agctgaagaa
     1501 acggttctcc atctcgcagg accacgagtt tcgctcgatc cgtcccgtcg gtgtctcata
     1561 gactgatcga gagctgccct tcgggaagcc tgctgattca ataattccac gcccgtccga
     1621 tagatcaatg accatcgcaa taaccttcat ctgaggcaaa tttcagaccc atattggatc
     1681 acgcaccaac acgattagag ccaggcgaat acacgaggac gactactcag accaggcacc
     1741 ggcagttcat gggccatccg ttgaacgatt gatcgttcat tcgatggcca cagcataatc
     1801 gtgcccttcc cactggtcgg ttgccgtcca gaaaaaggta aagcgaatcg gcgccctctg
     1861 cgtgggacgg atcgggatgt ccacaaactc aatcttaagt ctcgtgacga tcgagggcgt
     1921 ctcctccacg gtgcgccatt cgtcggctga ccaatgcaat cggaaggagg ccgggacctg
     1981 aatgcggagg gtctcccctc gcttcacgcg gcgcacctgc cggttcggct tccagacttc
     2041 gaggctgcgc cgatctggac gtgccccgag atagcgggtc gcgacgtcag ggatgaggtc
     2101 gaagacgttg ccatcgacga cggaccggag gagcttgatg tactccgcat gcgcccacac
     2161 gagcggcatc gcggagccgg tcggccgacc gcacgatagg taatgccttg gccgatccga
     2221 cttatcccac acttgttcgg gtaacagacc ggtgggtgcg gcgaacccct ccatggctct
     2281 gatgaaggga gtggcatctc gtccagccgc caactcgtaa tgcccccgtt cgcccgtgag
     2341 caacggccac gcgcggcccg tgccccatcc cacgaacggt ccgctatcgt ctcgttgtcc
     2401 gtacccatcg tggttgtacc gatgccagca cggcccgaag ggggtgttca ctttgagcag
     2461 cccatccacc actgcgagag aatccacaat gaggggatca tgcggcggtc ggatgccgta
     2521 tcgcaccagc tcaacaaacc cggcatccac aatgtctctg gccgcaaaga cacgttgagc
     2581 ctcaggtgga cggttcgcaa tactgagctc gccccggttg gggtcttctt cagggtgggc
     2641 attgtcgaca gcctccggta gaatccgtat atagtgtcgg ctgatgccgg ggacaagaga
     2701 tccctccgtg gtcacggtcc acgcctcaag atgcgcttca agaaaatcgg cgtattcttc
     2761 taaaaaggtg gccgtggcat cgtcgcctcg gtcgcggcta aatcctgccg cgccgatcaa
     2821 tgcggcaatg ttggaggcga gggtggacgg cgaatacccg ctcacttcct cccaacgttc
     2881 ttgctgtgtc acgggtccat ggcggaccag atatgcggcc gcctgaagga ccatgggata
     2941 aggatcaaag ccttgtagag cctcgtgttg tttgagccgc cacgcgagca cgatcgaaca
     3001 ggccacttcg tccagttgaa tgccacgcca gtaggggtct ccatcgatcc agaaattctg
     3061 agggaaacca ccgtcctgat gctgggcgct cgcgagatag atgagcgcgc gcaggggagt
     3121 ttcacgatgt ccggcggcaa gaagggcggt ggcgctgtgg accatatcgc gcggccatac
     3181 caagtgatat cccccttgat cgccatcacc tttcgcgtct ccccaaggaa tcgacaggga
     3241 cgcgataaac gcaccaggat aggatttgtc ttcgtgcgcg agcagcacac cgacacttcc
     3301 atgatagagt cttccctcgt cactggacgc gcgctctaac gggtgtcggt gagccgccgc
     3361 acggtgccat tgatctgtgt atcgcgtgag gtggtcgtgg aaggggaggc tcaacgcctg
     3421 gaacacggtc gtcaatgcct ggtgctgggt gcgaccaaag gccaggccca gggtgaactc
     3481 ccggcgacct tccaggtcga gttctcctgt gagcgcgata ttgccgtccg gggcatggtc
     3541 gaactcccag tccatgtgga agttgtcggc caagtctgtc cagccgtcac tgtagcccac
     3601 atacccacag gacacacgag caaaggggac cgtcgccatc agcgccagcc aggttccctc
     3661 tttctcagcc atcaagattt tgtggccggc ggcttcgatc accgaaccgt tgtttcccca
     3721 cccgccgatc tcgagatgcg gcgcacacag aacatagaga tgcagcgccg agagaaaggc
     3781 gtcatcaccc gtgagttgcg tccgctggag cacacagggg aggtgcgggg ccgtgatgat
     3841 ctccttcaca atggcgtaac ggccggcggg gtcggcgttc gtacagcggt accccaaggc
     3901 atagtccgac agccgttcgc aggaggacgt gaggtggcgt ttttcttcct ggacaaaact
     3961 gattccgtcg ctgatgagat attgccaatc tcgaagttga ggtcgatcga cggtcggata
     4021 atagacctcg gtcacgatac cgttccacat agtgaaccag atgcggctgg acgtggcata
     4081 agccgtccca accccatctt tgcctccatg tgtccatcga ggctcgatac cgggatgacc
     4141 aaacgccatc ttgacgagtg tagggttcat gctgtgtctc ctccccggtt aacgacgagg
     4201 gcggccgctt ctcgatccag ccaccagatc accgttccct gagcgagacg aaccccggac
     4261 gccgggtagt gcttcgggtc gggtgaccca aggaggatgt gcggcagggc ttgtgccttg
     4321 ttcgatcctg tcaccagaaa gtaggcgttg gcagcgtgag tgagtgcggg caacgtcaac
     4381 gttaggcgca ggtgtggctc gactggggcg tccacggcga ccacccagcg tttcgtctcc
     4441 gcgagggcag gtgattcagg gaaaagcgat gccgtatgcc cctcttctcc gagcccgaga
     4501 aaaaccaggt tgaaacgcgg ccagtctttg gagaaatggt tccgtagcgt tttctcgtag
     4561 tcccatgccg ctacgtcggg ggcccacagt tgtgtccgca taggatgcac atgctcaggc
     4621 gggcacggaa cggcatcgag caatgtctcc cacgccatgc ggtagttact gtgaggatcg
     4681 tcaagtggca cgcaacgctc gtctccccaa aacacatgca cgtgcttcca tgggatctgg
     4741 tctcgaaact gcgaagaaag tatgcggtag agggtgcgcg gcgtgttccc accggagaga
     4801 gcaagagaaa cgctgccatt ggtttgcacg gtctcattga tgattctgac cgtcgcctcg
     4861 gccagttgac ggctgagctc attcacatcc ctaaagacac gtacatccgg tttcatgtcc
     4921 tctcctcttg attcctcacg gctgaccaac tcagcctcca cagatcactt ctgtctcgtg
     4981 atccgacaat cgggggatcc acttctgtgg tgacgacccg aacaagtcgt catcggatag
     5041 gtgctcgagc ttcaagggct gcttcagcag cggattgtcc agcagataga cctgtcctac
     5101 cgagagataa ttggtggccc gccaataggc gtccatctgt ttcagtaagg ctgcgtccga
     5161 ttgaggcatg tttttgttca tcatcaatga ccccctcagc gatccatctt ccccgtgctc
     5221 cgcaacagag ccatggactc cgcgatctcg cgctcttcat cagtgggaat cacgtgcact
     5281 ctcacgcggc ttgcttcgga tgaaataacc ggttgattag cctcattggc cggtttattg
     5341 atgacgatgc cgagaaattc taatccggcg cagatgcgcg ctcgaatcgc aggtgcatgc
     5401 tcaccgatcc cggcgctgaa gaccaatgtg tccaatccac ccaacaccgc cgtcaacgca
     5461 ccgatccatt ttcgagcctg atagcagaac aatccaaccg cctcggctgc ccgcggatca
     5521 ctctgctctt gcttgaggag gtctcgcatg tccgaactca cctcggacag gccgagcaat
     5581 cctgattcgg tattgaccat ctcgtggaac cgctccacgg tcatcccttc tgtgcgagcc
     5641 aggtaggaaa cgagtcctgg atcgaggtcg cctgctcggc ggctcatggg caagccggaa
     5701 gcgggggtga aacccatcgt cgtatccatc gcctgcccgt ccttgaccgc cgccatgctg
     5761 gcgccgtttc cgagatgggc aagaatgata cggccctctg cctcgccagg cgcacccact
     5821 cgtttcagct ctcgcataag aaacgcatac gacaacccgt gaaacccata acgctgaaca
     5881 cccagcttct catatcgcct cggaatcggc agcagtcgcg caaccagcgg catgtcccgg
     5941 tgaaatgccg tatcgaaaca ggcgacttgg ggaagagtcg aatcataccg atggaacgat
     6001 tcgatcaagg agatttccgc gggcagatgc tccgggctgt agtggcatag tcgtcgaagc
     6061 tctgtcaaca actcgggcgt cactcgttcg ggtctggtgt aggtcttccc gccgtgaacg
     6121 acacgatgtc cgacagcaca gagcctccca atgccgacct gttgctccaa ccattcggtg
     6181 ataaccccca cgcaggtccg atggtccggc gcgcgcacgg cccgtcgact cggttgttgc
     6241 gtgcccgcgt cagtgacgag catctcactg ttcggcaggc caatccggtc gaccatgccg
     6301 cgtgccaccg gcgcggagta atccgcatct cgaaacagag catacttgat gctggacgat
     6361 ccgccgttga tcgtgagaat gaacgagctc ggttcctgtt gattcatgtt gtcagccatc
     6421 ttggcagacg tctaaagttc agaagcaccc gcctccattc actcgcgggc gaaggagtgt
     6481 atcgatgcat gactggggta cctatgcgca caatgcctat catcaagcac atggttcgct
     6541 cacacgtgcc gttagattca ggttcaagac aactcaccca gtcacgatcg gattatgcca
     6601 gcctccagga ggggacaggc tctgggtgac gtgactcggg ccccaggtat tcggttcgta
     6661 ttcgtggacc ggagtgccct gcttcagcac cggatcgacg atgcgccacg cctcttctac
     6721 atagtgttcc cgcgcgaaca gggtccgatc acccgtcatg gcatctccga gcaaccgctc
     6781 atagacatcc agctcatcgg cggtgggccg ggtgctggcc agcatttcca ccgattgacc
     6841 gatcatcttt tcctcacggt ccatcacgat cgtgccgagc gcgagggtca cttcggggtt
     6901 gactcggaag cggaagtagt tcggcggggc cgagcatgaa gggaaaatgg tcggcggccg
     6961 ccgcaagcgg atcaccacct ccgtgcaggt cacaggtaga cactttccgg cccggatgta
     7021 gaatgggacg ccttgccagc gccacgaatt caccgctaac cgaagcgcga cgaacgtttc
     7081 aacctgcgag tccggcgcga cgccctgctc cgttcgatag cctcggaatt gtcctcggac
     7141 cagggcggtc tcatcgagtg acggaatcgc cctcagaacc ttgagctttt catcacggat
     7201 cgattcgctg tccgttctaa ccggaggctc catcgccagg agggtcagga cctggaacaa
     7261 atggttctgc accacgtcgc ggatggcgcc cgtctggtcg tagaacgccc ctcgtccctg
     7321 gacgccgaag gtttccgcca tggtcatctg cacgctctcg atatggtcgc ggttccagaa
     7381 cggttccagg aacgcattcg tgaaccggaa gaagagcatg ttgtgtatgg gccgcttccc
     7441 gaggtaatgg tcgatgcgga agatcgcggc ctcgtcaaag ctgtcgagca gaatctcgtt
     7501 cagtttcttc gctgagctga ggtcgtgacc gaatggtttt tcaacgatga cgcgagcgcc
     7561 gtgggtgcag cctgcgtcgg cgagttgttc cacgacagac ccgaacaaca taggcgggat
     7621 ggcgagatag tgcgccggcc gctgtgcgga gccgagctct ttgcgaacca gctgataggt
     7681 cgcgggatcg ttgtagtcgc cgtcgacata tcgcagcaac ccgcacagcc tgtcgaacgc
     7741 cgcgggatcg actccgccgt gttgctgaag gctgtcacgc gcccgcgccc gaagctgctc
     7801 gatggtccac ccgtttttgg ccacgccgat aaccgggaca tcaagggagc cgtgtttcac
     7861 catggcctgc agggacggaa aaatcttctt gtaagccaga tcgcctgtgg cgccgaaaaa
     7921 gaccagcgcg tccgaatggg gtccgttcat ggtgtcgtcc tcatttcgta gattgttcca
     7981 cgtgtccccc gaactcatag cgcatcgccg agagcaactt gtcctggaaa tccgattcac
     8041 cgcgtgaact gaatcgttcg tagagcgcgg ccgtgagcac cggcaccgga acagcctcat
     8101 cgatggccgc cttgatcgtc cagcgtcctt cgcctgaatc cgatactcga ccggtgaatt
     8161 ccgagagggc ggggttcttg agtaacgcgg atgccgtcag gtccagcagc catgaggcga
     8221 tcacgctgcc gcgccgccac acctctgcga tgtccggcag attgaggtcg tactgatagt
     8281 gcgcagggtc acgcaaggga gttgtctcgg cgtcgatcgt ctgtgtcctc ttgccgatat
     8341 tggccgctcg caggacgccc aacccctcgg cataggcggc catgagcccg tactcgatgc
     8401 cgttgtgcac catcttgaca aaatggccgg cgccgtttgg accgcagtgc agatagccct
     8461 gctcggcggt gccgtcgatc ttctcccgtc ctgctagccg cggcgccgcg tcccgccccg
     8521 gcgccagccg cgcaaagagc ggatccaggt gcttgacgac cggttcctcg cccccgatca
     8581 tcatgcagta acctcgctcc agcccccaga ccccaccgct ggtccccaca tcgacgtagt
     8641 ggatccgctt cggcatgagt tccttggcgc gccggaggtc gtcgacgtag taggaattgc
     8701 cgccgtcgat gagaatgtcc tccgcctcaa gatggggcag aaggtgagcg atggcttggt
     8761 ccacaccagc cgccgggacc atcaaccaga tcgcgcgagg cttttcgagt ttcttcgcaa
     8821 gatccgggag cgaagcaacc cccacggcgt gttccttgac caactcctgc accgcattcg
     8881 gcgaccgatc gaagaccacg cactgatggc ctcctttcag cagccgtcgc accatgttcg
     8941 ctcccatccg tcccagaccg atcattccaa gctgcataga tcacctcctt acagatgtcg
     9001 tcactcgact ggttgaagtt gtttccacaa gccacatctt tgctttcatt aaaaatcgct
     9061 gttccggcca ctagaacatg agctccagcg gcaaccgcca acggagcagt ttccgcattg
     9121 atgccacctc cacctcgtag ccttgcgtgc gctcatcaat catctcgcgc acacgcccac
     9181 gtcgatgtgg atgcgatcgg cgccggcctg ctcc
//
LOCUS       DATMTI010000496         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_485963, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000496 DATMTI010000000
VERSION     DATMTI010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485963"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VN039_08005"
     CDS             <1..193
                     /locus_tag="VN039_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45945.1"
                     /translation="LTSFHGHEWTPMIFESDVHVPFFVILSEVENRAAGEDARRTARP
                     KAERVGSERIKSLEIFGRL"
     gene            190..>1647
                     /gene="thiC"
                     /locus_tag="VN039_08010"
     CDS             190..>1647
                     /gene="thiC"
                     /locus_tag="VN039_08010"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HWV45946.1"
                     /translation="MNTNNGSEESKAAEVSLPNSTKIYVAGTLHKDIRVPFREISLAP
                     TNSTNGELEINEPVRVYDTSGPWGDPDFHGDVTQGLPPLRGKWIRERGDVETVVGRPI
                     KPADDGWLSEAHAAHSSRKRPTPKLQLSTSNSEGRDVQSDNSAFGAPRKILRAKSRAI
                     TQLWYARQGIITPEMEFIAIRENGRQIENRESRIGENGSSNAQFTNHDSRNHLRFAHR
                     GESFGANIPAEITPEFVRAEIARGRAIIPANINHPESEPMIIGRNFLVKINANIGNSA
                     VASSIEEEVEKMRWATKWGADTVMDLSTGKNIHATREWIIRNSPVPIGTVPIYQALEK
                     VGGRAEELTWEIYRDTLIEQAEQGVDYFTVHAGVLLRFIPLTARRATGIVSRGGSIMA
                     KWCLAHHQESFLYTHWDEICEIMAAYDISFSIGDGLRPGSIADANDEAQFAELYTQGE
                     LTRRAWAQHVQVMNEGPGHIPMHMIKENMEKQLEWC"
BASE COUNT          417 a          426 c          472 g          332 t
ORIGIN      
        1 gctgaccagt ttccatggac acgaatggac accaatgatt tttgaatccg acgttcacgt
       61 gccgtttttt gtcatcctga gcgaagtcga aaatcgagcg gctggggaag acgcgagacg
      121 gacggcaagg ccgaaggctg aacgagtggg aagcgagcga atcaaatctc tggaaatttt
      181 tgggcgccta tgaatacgaa caacggatcc gaagaatcga aggcggctga ggtgtcgctc
      241 ccaaactcca cgaagattta cgtcgcgggc acactgcaca aagacattcg cgttccgttc
      301 cgcgaaatct cgctcgcacc tactaactcc accaatggtg aactggagat caatgaaccg
      361 gtgcgcgtgt acgacaccag cggtccatgg ggcgatccgg attttcacgg agacgtcaca
      421 caaggacttc cgccgttgcg cggaaaatgg attcgcgagc gcggagatgt agagaccgtt
      481 gtgggtcgac ccataaagcc ggcagatgat ggctggttat ccgaagcgca cgctgcacat
      541 tcgagcagaa aacgcccaac gccgaagctc caactctcaa cgtccaattc agaaggccga
      601 gacgtgcaaa gtgataattc ggcgttcggc gcgccccgga aaattcttcg cgcgaaatcg
      661 cgtgctatta cgcagctttg gtacgcgcgt cagggaatca tcacgccgga gatggaattc
      721 atcgccattc gggagaatgg ccgtcaaatc gagaatcgag aatcgagaat cggggaaaac
      781 ggttcttcga acgcacaatt cacgaatcac gattcacgaa atcaccttcg cttcgcccac
      841 cgcggtgaat cattcggcgc gaacattcct gccgagatca cgccagaatt tgttcgggcc
      901 gaaatcgcac gcggtcgcgc tatcattcca gcaaacatta atcatcccga atccgagccg
      961 atgatcatcg gacgtaattt tttggtgaag atcaacgcga acatcggcaa tagcgcggtg
     1021 gcctcgtcga tcgaagaaga agtcgagaaa atgcgttggg cgacaaaatg gggcgcggac
     1081 acggtgatgg atttgagcac gggaaagaac attcatgcca cgcgcgaatg gatcattcgc
     1141 aacagtcctg tgcctattgg aaccgtcccg atttaccagg cgctcgagaa agttggcggt
     1201 cgcgcggagg aactgacctg ggagatttat cgcgacacgt taattgaaca ggctgagcag
     1261 ggtgtcgatt attttacggt gcatgcgggc gtgcttttac gcttcattcc gctcaccgcg
     1321 cggcgtgcta ccgggatcgt ttcacgcggc ggttccatta tggccaaatg gtgtctcgcg
     1381 catcatcagg aaagctttct ctacacgcat tgggatgaga tatgcgagat catggctgcg
     1441 tacgatatca gcttttccat tggtgacggt ttgcggcctg gctcaatcgc cgacgcgaat
     1501 gacgaagcac agttcgccga actttacaca cagggcgagc tgacgaggcg ggcctgggcg
     1561 caacatgtgc aagtgatgaa cgaaggcccg ggtcacatcc ccatgcatat gatcaaggag
     1621 aacatggaaa agcagctcga gtggtgc
//
LOCUS       DATMTI010000497         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_825746, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000497 DATMTI010000000
VERSION     DATMTI010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825746"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1142)
                     /locus_tag="VN039_08015"
     CDS             complement(72..1142)
                     /locus_tag="VN039_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV45947.1"
                     /translation="MYKTALYLPGRPSRFAERPYLPGRTRMEVLSEVLKAVKLNGALF
                     YNAEFSAPWCAQSVDAHSVTSHLSPKSEHVIIFHLLTEGRGYANLKDDDRLVPLNAGD
                     ILIVPNGDPHILGNGPPVRPVERGQVLDQVLSQGLKVSRMGGGGELTKFVCGYMTCEP
                     QMGQVFLGGLPPMLKVSIRDDASGHWLEQSIRYSVDTVDPARPGGETVLAKLAEVLFV
                     ETLRRYIALLPPGQTGWLAGVRDQEVGKALALLHRNPADPWTIASLAKGVGLSRSVLA
                     ERFQRYLSETPIAYLTRWRLQLGAQMLTSTSSSVAQIAAEVGYESEASFNRAFKREFR
                     LPPARYRSQSRASRNTPSAMHQ"
     gene            complement(1692..>1884)
                     /locus_tag="VN039_08020"
     CDS             complement(1692..>1884)
                     /locus_tag="VN039_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45948.1"
                     /translation="SLTMALKEEQLLIAIEGARSGSNGSKSEDRPAASCNIYFYMQVG
                     ASVLQDKVVRHKQALILKY"
BASE COUNT          414 a          503 c          555 g          412 t
ORIGIN      
        1 gttatagtgc tcggaggtcg gcctcatttt cagcatggcg gattcctcta tcgtttcgtt
       61 gcgtcggctg ctcattggtg catggctgac ggggtgttgc gggatgctct cgattggctg
      121 cggtaccggg ccggcgggag gcggaactcc cgtttgaagg cacggttaaa ggacgcctcc
      181 gattcatagc cgacctcagc ggcgatctgt gcgacgctgc tgctcgtgga tgtcagcatc
      241 tgcgcaccaa gctgaagtcg ccagcgcgtg agataggcga taggggtttc cgagagataa
      301 cgctggaacc gctcagccag gacggaacgg gacaggccga ctcctttcgc gagtgaagcg
      361 atcgtccaag gatcagccgg gttgcgatgc agcagagcca aggctttccc gacttcctga
      421 tcgcggacac cggccagcca accagtctgt cccggtggga gcagcgcaat gtagcgtcgc
      481 agcgtttcca cgaacaacac ttcagccaat ttagccaaca cagtctcacc gcccggtctc
      541 gccgggtcaa cggtatctac cgaatagcgg atggattgtt ccagccagtg gcctgacgcg
      601 tcgtcgcgaa tgctgacctt caacatgggt ggcagcccgc caaggaacac ttggcccatc
      661 tgtggctcgc aggtcatgta gccgcagacg aatttcgtca gctccccacc tccgcccatg
      721 cgggacactt taagaccttg agagagtact tggtccagca cttgtccacg ttccacaggt
      781 ctgacgggtg gaccgtttcc taaaatgtgt ggatcgccat ttggaacgat gaggatgtcc
      841 cctgcgttca gtggaaccaa ccgatcatcg tctttgagat tggcatagcc gcggccctcg
      901 gtcagaaggt gaaagatgat cacgtgctcg gacttcggcg aaagatgcga agtgacactg
      961 tgcgcgtcga cggactgagc gcaccagggt gctgaaaact ccgcattgta aaacagggct
     1021 ccgttgagct tcacggcctt gaggacttcg gagaggactt ccatgcgtgt tcttcccggc
     1081 aggtacggac gttcggcaaa acgcgacgga cgcccaggca agtatagtgc agtcttatac
     1141 atctgagtca agagaaccag actcctggac aagagcccac ccgaagactc gccttatagt
     1201 gcgacacgta gttgaagcgg tgctgccaac ccgctgtcga ttcaatgacg caaaggagga
     1261 acacatgtcg gtaagagctc tcgtatccga gtagagaggg tatgagggtt ttggccgcat
     1321 gtctggaagt catcggcgtt cgagcagtga atatctatcg taagaacggc tgggcatttc
     1381 agtgaagagc aaatgaggga catggcgctt cttttcacca actcacaaag gcacagagca
     1441 tgaacataga ttggcgaaaa cataaaacgg tcgcagttgt cacgtgtcga ctgatccccc
     1501 ccaagatcgc agtcgcagtc ttggggggca tatggctcct tcaggtttca gtcgttgagt
     1561 ccttgccctc tgctgcgata accgttgtag tggaacctgg cacagatggt gtgcaacggg
     1621 caacgatcgt gatgacgagt tattcctaca ccccggacca tctggtgttc catgtgggca
     1681 gtccggttga gttaatactt gagaatcaga gcttgcttgt gccgcacaac tttgtcttga
     1741 aggacgctgg cgccgacttg catgtagaag tagatgttgc aggaggcggc agggcggtcc
     1801 tccgatttac tcccattcga tccggaacgg gcaccttcta ttgcgataag cagctgttct
     1861 tctttaagag ccatcgtcaa cgag
//
LOCUS       DATMTI010000498         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_697275, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000498 DATMTI010000000
VERSION     DATMTI010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697275"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..503)
                     /locus_tag="VN039_08025"
     CDS             complement(63..503)
                     /locus_tag="VN039_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45949.1"
                     /translation="MITSLADTKVVLPHSQRFADTGRDFLLQTTIPGDFKTLEGVVWG
                     LLALSSQCPNKDFSAPFFPDTISSTSQHEGAQPLYMYYRGLRRNGRKVIVRFTGDAYR
                     YLNNTAAIQELVKGALEATIRLHTNKSTTVDYEIDGEIVDEWDA"
     gene            complement(597..1025)
                     /locus_tag="VN039_08030"
     CDS             complement(597..1025)
                     /locus_tag="VN039_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45950.1"
                     /translation="MKLHRLIPALFALTAVLTPQLPGTATAQSDKPKYGAKLISPRPG
                     QTLQPGQVVRIQWTANFPNVDLSMCETEVLLSLDGGKTIYTMISEQRNPRIQYFDWTV
                     PNLPTRQAVLDIRFGCLNIYPETSSLQVQAPFVIGSAPAL"
     gene            1262..>1592
                     /locus_tag="VN039_08035"
     CDS             1262..>1592
                     /locus_tag="VN039_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45951.1"
                     /translation="MAIIESCQVHRARVAAQGSFAAQIKVNVEVTHRQLAQGAVNRFA
                     ITATREIGFCHRAPVAAHFEDRNDMIGVLFCFQIKDQWWEPDYAQRGRSENSTIETGR
                     DPVTQNFF"
BASE COUNT          379 a          401 c          425 g          387 t
ORIGIN      
        1 gttcggatac cgatcagcta cgaatgagat gacttatatc cggttcgaat ccgggcggtt
       61 tatcatgcat cccattcatc gacgatctcg ccgtcgatct cataatcaac agtcgtcgat
      121 ttgttagtat gcagtctgat ggtcgcctcc agagcgcctt tcacgagttc ttgaatggca
      181 gcggtattgt tgaggtagcg ataagcgtca ccggtgaaac gaacaattac ctttcggcca
      241 tttcgccgca aaccgcgata atacatgtag agaggctgcg caccttcgtg ttgcgacgta
      301 gaactgatgg tatcgggaaa aaacggcgct gaaaaatctt tgttcgggca ttgcgacgac
      361 aacgcaagta atccccagac gacgccttcc agcgttttga agtcgccggg gatggttgtt
      421 tggagaagaa aatctctacc tgtatcagcg aaccgttgag agtgcggtaa aacgaccttc
      481 gtatctgcca gcgaggtaat cactggacaa atgacaatca atatggctgc ggctatgcga
      541 gtgttcatag acctaaccaa caacctaacc agttcgtcgc caatttccgg aggagactat
      601 aaggctggcg ccgagccgat cacaaaagga gcttgaacct gaagactgga agtctcggga
      661 tagatgttta aacagccgaa ccgaatgtcc agtaccgcct gcctggtggg cagattcggg
      721 acagtccaat cgaaatattg aatcctcgga ttacgctgct cactaatcat ggtgtagatc
      781 gttttccccc cgtcgagcga caggagaacc tccgtctcgc acatcgagag gtccacgttt
      841 gggaagtttg ccgtccattg aattctcacg acctggccag gctgcaaagt ctggcccggt
      901 cttggcgaaa tcagttttgc tccatatttc ggtttgtcgc tctgcgccgt tgcggttcca
      961 ggcagttgtg gagttaaaac agctgtcagc gcgaacaggg ctggaattag tcggtggagc
     1021 ttcattctct tagtccatcc gagtggcggt tgattgtcaa tcacagtttg cgccgcaaga
     1081 acttgctaat ttgtcatttc ttacgccggc gacttgttct gtgtccaacc atttccccgc
     1141 gcatcgttcg ccgatcgact cgcgtgagat tttttgccat tgcgggtatt aataatgaaa
     1201 tgtgcggaat gtcgcagaga tgtgcagcga taaaccagat gggtttgtcc gtggtcatcc
     1261 aatggcgatc atcgaatcgt gtcaggttca ccgggcgcga gtagcggcgc aaggttcgtt
     1321 cgccgcgcag attaaagtaa atgtggaagt aactcatcgc cagctcgcgc agggtgcggt
     1381 aaacaggttc gcgataacgg caacccgtga aattggattt tgccaccgcg ccccagtggc
     1441 cgcgcacttt gaagatcgca acgacatgat cggtgtcctg ttctgcttcc agatcaaaga
     1501 tcaatggtgg gaacccgatt atgcgcaacg cggccgcagc gaaaattcca ccatcgagac
     1561 aggacgcgac ccggtcacgc agaacttttt tc
//
LOCUS       DATMTI010000499         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_612659, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000499 DATMTI010000000
VERSION     DATMTI010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612659"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1048
                     /locus_tag="VN039_08040"
     CDS             320..1048
                     /locus_tag="VN039_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45952.1"
                     /translation="MATRCEAGARTGRAGATDGKVSADGIRYMVRTPSNYDATFAHPL
                     LMVYAPAGQSRWTSERLTGLTTAATGAGFVVIYADHRQLNISAIEQLGTIPGLVAKEW
                     CVDEHRVYVTGHSDGGTASLMLSVLEKTKKVPAAIAPSAAGVTGKDLDAFQCPAPIPV
                     MIMHSKDDNLFPGWGSQTSAWWAGCNHCDVTKTKPVEGGCRAYQGCTSGGATLYCEGT
                     GSHRDWPNLNRVILVFFTHPEKFQ"
     gene            1098..1706
                     /gene="plsY"
                     /locus_tag="VN039_08045"
     CDS             1098..1706
                     /gene="plsY"
                     /locus_tag="VN039_08045"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HWV45953.1"
                     /translation="MDQTGLIIGLVICGYLLGAVPFGVVISKAMGLPDPRTVGSKNVG
                     FTNVLRVSGKKAGILTLIGDMGKGWVMGFAATQLLQDEWSILIVAVAPFLGHLFSPFL
                     GFKGGKGVATALGSVLGVAPLIGLVLLLAWIGAVAIWRYSSGGALTAFGLFPILAASL
                     RPTAPFALFSMLVTALIVIKHKGNIERLWKGTESKMGQGRPG"
BASE COUNT          405 a          536 c          570 g          443 t
ORIGIN      
        1 ggacactggg cgctcaccaa cgcgcgcccg tccgtaaaca tgacgctcat tattcttcgc
       61 gtcgcggacc tcgctgcggc ctcgctggat gtctagtttg aacatcctgt agtgcgttgg
      121 attggactcc tcgccaactc ccttcagcat tgactatcat caggacgaac ctgacatggg
      181 tttgaaatgc caggccgtcc gccgaacgcc actcctacag cagatcgttg tctgtagctg
      241 tcttttcttc ctagtcgcag cctgtacaga gagtggaacg cctcctcaat tagaagccta
      301 cgcctattca gatgctccag tggcgacccg atgtgaagcg ggcgcacgta ctggtcgcgc
      361 aggcgcaacc gatggaaaag tctcggctga cggcatccgg tatatggtcc gcacaccatc
      421 gaactatgac gcaacatttg ctcaccccct cttgatggtc tatgctccgg cgggtcagag
      481 ccgttggaca tcggagcgac tcacaggtct cacgactgca gcgaccggcg ccgggtttgt
      541 cgtaatctat gctgatcatc ggcagttgaa tatctctgct atcgaacaac ttgggaccat
      601 tcctggtctg gtggcaaagg agtggtgtgt ggatgagcac agagtgtatg tcaccggcca
      661 ttcagacggg ggcaccgcat ccttgatgct atccgtgctc gaaaagacga agaaggtacc
      721 ggccgcgatc gcaccgagtg ccgctggggt gaccggcaag gatctcgatg cttttcaatg
      781 tccggctccc ataccggtca tgattatgca cagtaaggac gacaatctct ttcccggatg
      841 gggaagccaa acgtcagcct ggtgggcagg ctgcaatcac tgcgatgtca cgaagacaaa
      901 accagtggaa ggcggatgtc gcgcgtatca aggatgtacc tcaggcggcg cgacgctcta
      961 ctgcgaagga accgggagtc accgggattg gcctaatctc aatcgggtga tactcgtttt
     1021 tttcacacac ccggaaaaat ttcagtaatc gtcgacagca tcgctcctat ctaaatgctg
     1081 atagcgtcga gagttgaatg gatcagacag gactaatcat cgggctcgtg atctgtggat
     1141 acctgctggg tgcagtcccg tttggagtcg tcatctcaaa agcgatgggg cttccggatc
     1201 cccgcacagt cgggagtaag aacgtcggct ttaccaatgt cctccgtgtg tcggggaaga
     1261 aagccggcat cctgactctg atcggcgata tgggcaaagg gtgggtgatg ggcttcgcag
     1321 cgacgcagct gctgcaagac gaatggtcta ttctcatcgt tgcggtcgct ccgtttctag
     1381 gccatctctt ttcgccgttc ctaggattca aaggagggaa aggtgtcgcg accgcgctcg
     1441 gctccgtcct cggtgtcgca ccgctgatcg gcctggtgct ccttctggcg tggatcggag
     1501 cagtagcgat ctggcgctac tcctccggcg gcgcactgac ggcgttcgga ctctttccca
     1561 tccttgctgc atctcttcga ccgacggccc cctttgcttt gttctctatg ctggtgactg
     1621 cacttattgt gatcaagcac aaggggaaca ttgaacggtt gtggaagggg acggagagta
     1681 agatggggca agggcggccc ggttgatcca tgtgctcgcg gaacccccac gtctgctgat
     1741 catatttcgc tgaaggagaa cgacatggtc gttccatgcg cgcaattctg gattcaaccg
     1801 ggccgccctt gtgaagaggg aagtgaaggc tgtgatccac agccgcaaga cgatagatgc
     1861 aggtgttagt ccgaacagag agggttaacg ctaagctcag ccggcgctgt acgcgtccgc
     1921 tgcggctccg gattaggcac cgatgatttc attc
//
LOCUS       DATMTI010000500         8210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_825886, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000500 DATMTI010000000
VERSION     DATMTI010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825886"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /gene="ettA"
                     /locus_tag="VN039_08050"
     CDS             <1..1104
                     /gene="ettA"
                     /locus_tag="VN039_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HWV45954.1"
                     /translation="EPTNHLDAESVQWLEQHLQQYKGTVIAVTHDRYFLDNVAGWILE
                     LDRGHGIPFQGNYSSWLEQKKDRLEKEEKAESKRKKTLEHELEWIRMSPKARQSKGKA
                     RLNRYEELVNQKQDQVTEDLEIYIPPGPRLGDVVVEANGISKAYGDNVLYESVNFSLP
                     KGGIVGVIGPNGAGKTTMFKMIIGKEKPDAGSIKVGETVKLGYVDQDRSLDGNKSVYE
                     VISDGQDTVKLGKTEVNARGYCARFNFAGTDQQKKVKDLSGGERNRVHLARMLKEGAN
                     LIILDEPTNDLDVNTLRSLEEGLDNFAGCAVISSHDRWFLDRIATHILAFEGDSKVVW
                     FEGNYSEYEADRKKRLGKEADQPHRIRYRKLTR"
     gene            1355..5122
                     /locus_tag="VN039_08055"
     CDS             1355..5122
                     /locus_tag="VN039_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV45955.1"
                     /translation="MSNKRSDKAATRMPPIDHDCWTFAFKGSGIGLWDWNVAINKVIF
                     SDGWKDLFGYAQDEVSDNFNEWSSLIHPEDRSTVIANIQRYIQGEIPVLTSEHRIRCK
                     DGSYKWVFERGKIVSRTKDGNPLRVLGIHTDITERKQLARRLAIQHEVANVLAGASGL
                     DNAISAILQPVCQTLGWNEGLLWVVDDSTQRLRCHSIWTASGTASERYGAASRELTFQ
                     CGVGLPGRVWADAKPVWISDVTRDENFPRAALAEQVGFHTAAAFPVRLADRVYAILEF
                     FHHEILPADSPLLTTFQAVADQLSQFCARKQAEERLRQTQFAMDQAVDAIYWVDPQAR
                     ILYANEAASFMVGYSREELLGMTVHDLNPDFPVSRWPGFWEEMRRNKTMSIETHHRAK
                     DGRLVPIDIRVSFLAYEGQEFHCAFVRDITERKRANEALEQIMHRYKDLINSINGIVW
                     EADASTTQFTFMSPQAETILGYPVEQWLSSPNFWVDHMHPEDRDWAPQYCLEEVRKHR
                     AHTFEYRMLAADGRIVWIRDLVSVLIENGRVTKLRGIMEDITDRKRIEDSLRDSEERF
                     RTFLDHAPNAMFMKTNDGHYFFTNQCFEQLCHSDREHVVGKTDVELFPRETAEQFRAN
                     DRQVIETGKAIEFEETNDLHTNIVVKFPVRDRLGQIYATGGIVTDITARKQMEFKLQQ
                     FAAEMERQNLELAEAHMQALAATKAKSEFLASMSHEIRTPMNGVIGMTGLLLDTPLTL
                     EQREYAETVRLSGEHLLDIINEILDFSKIEAGKLDLEELNFDLHATMEEAVGLLGERA
                     YAKGLELTYLVQAGVPTALRWDPGRLRQILVNLIGNAIKFTEQGEVVVTVSMEGNSSE
                     TTAASASSSYRTLRFEVSDTGVGITPEQQAKLFQPFTQADGSSTRKYGGTGLGLAICK
                     QLVEMMGGRIGVDSKVGEGSVFWLTVRFPLQPEGAQPVATIPASLRGRRILIVDDHAT
                     NRRVLEQSLRGLGVVYESAENGYQALECLRNAAGRQNHFDVAILDMQMPGMDGLELAH
                     RIKSESAISATRLVLLTSAGQRGDAKAAQSAGIAAYLTKPIRQSLLYECLGLVLGSVP
                     NSVAPTPQTTATIITRHSLSEAQTRSRPLVLLVEDNPVNQKVAANMIEKLGYRVNVAA
                     TGREAVDSLACIPYALVFMDCQMPEMDGFEATRVIRKQEESLRQVGGRQSHLPIIAMT
                     ANAMQEDRDRCLAVGMDDFLSKPVTSKSLAAVLDRWLPHDQAPAETESKAA"
     gene            5301..6182
                     /locus_tag="VN039_08060"
     CDS             5301..6182
                     /locus_tag="VN039_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV45956.1"
                     /translation="MKPRVIVTGAAGLIGQYCVKSASRWAPDWDVRGLSRTDLDLTDR
                     AAAEQAWRHLKPDAVIHYAALSRTKDCEQNPQLARRININATADLARLSKDIPFIFLS
                     SGEVFDGERAWYREGDAPNPINFYGKTKLEAERLVLQNPRHTVVRIVLTAGTSQNGDR
                     SFVEDMSRAARSGKSVTLYADEYRCPLPAGAIARAIWELVGKDESGLFHLGGHERLSR
                     WEVGQALLPWYPELQGRLVKGCAHDHPGAPRPADLSLNCEKIQACLSFPLPGFRSWLN
                     DRVHQGVDVWDYEHSLS"
     gene            6196..7029
                     /locus_tag="VN039_08065"
     CDS             6196..7029
                     /locus_tag="VN039_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HWV45957.1"
                     /translation="MEPENGIFASDPLPHVLAAYLFMTIVMGALWAVQRKTRNAAIGD
                     VGWCFGLILVVLWYATQASGGFARVFLPVMLVTLYAGRLGLHILFNRVIGKEEDARYR
                     RLREEWGESESAKMLAYFLLQALAVAVFSLPFLVLMWNPRPPSALVELIGLMIWGVAV
                     AGEARADRQLAHFRADPRNQGRVCREGLWKYSRHPNYFFEWLHWCSYVVMTLGAPGWV
                     FTWIGPIGMGLALLKLTGIPRTEAHALSSRGEEYKAYQATTNAFFPWFPRPRPDMATP
                     S"
     gene            complement(7103..7324)
                     /locus_tag="VN039_08070"
     CDS             complement(7103..7324)
                     /locus_tag="VN039_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45958.1"
                     /translation="MITARLVLCVFCLMLLGCSDQKAKELFETAAFEENQGNVPHAKQ
                     LYQELVNLYPSTKVAEIARARLADLDSRK"
     gene            7923..>8210
                     /locus_tag="VN039_08075"
     CDS             7923..>8210
                     /locus_tag="VN039_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWV45959.1"
                     /translation="MKFLAVHPGPLMYTKIYLRLEPLGLEMVAQACRQAGHDVRLIDL
                     QEDTWKDYEALIRTWKPDAVAFGCNYLANVPEIVDLAKLTKQELPNSFVFVG"
BASE COUNT         1866 a         2170 c         2420 g         1754 t
ORIGIN      
        1 gaaccgacaa accatctgga tgccgagtcg gtccaatggc ttgaacagca tctccaacaa
       61 tataaaggca cggtcattgc cgtgactcat gatcgatact ttttggacaa tgtcgcagga
      121 tggattctcg aattggaccg aggccacggt attccgttcc aaggcaatta cagttcctgg
      181 ttggagcaga agaaggaccg attggaaaaa gaagaaaagg ccgagtcgaa gcggaagaag
      241 acgctggaac atgaattgga atggatcagg atgtcgccca aggccaggca gtcgaagggc
      301 aaggcgcgat tgaaccgcta tgaagaactg gtcaaccaaa aacaggatca ggtgacggag
      361 gatctggaaa tttacattcc gccggggcca cggctgggcg atgtggttgt cgaggccaac
      421 ggaatcagca aggcctacgg cgacaatgtt ctgtatgaga gcgtgaactt cagtctgccg
      481 aaaggcggca ttgtcggtgt gatcggtcca aacggcgccg gtaagacgac catgtttaag
      541 atgatcatcg gcaaggaaaa gccggacgcc ggatcaatca aggtgggcga gaccgtcaag
      601 cttggctatg tcgatcagga tcggagcctc gacggtaaca agagcgtgta cgaggtgatt
      661 tcggacggcc aggacacggt caagctcggc aaaaccgaag tgaatgcgcg cggctactgc
      721 gcccgcttca actttgccgg aaccgatcaa cagaaaaagg tcaaagacct ctcgggcggt
      781 gaacggaatc gcgtccacct cgcccgcatg ctgaaggaag gcgccaatct cattattctg
      841 gacgagccga caaacgatct cgacgtgaat accttgcgat cactggaaga aggcttggac
      901 aatttcgccg gctgcgccgt gatcagcagc cacgaccgtt ggtttctcga ccggatcgcc
      961 acgcacatcc tcgcctttga aggcgacagc aaagtggtct ggttcgaagg gaactacagc
     1021 gagtacgaag cggatcgaaa gaagcgcttg ggcaaggagg ccgatcagcc gcacaggatc
     1081 agatatcgga agctgacacg ctaggagact cctcctcggt ccctcatatg gatgtcagcc
     1141 atgtccagaa ttgattgggg agcctgcatt tatggctctc tatgtctccc cttcgtccgt
     1201 tcactgcata ccgttgaatc cccaatccat ccggcttttc cgacaaggga aatggacctt
     1261 caattcccct caaggtattg agttaggaac cgatataagt cgagaggaag agaaagagct
     1321 atggggatct catcccgccg tgtgcggtgc tcccatgagc aataagcgtt ctgataaagc
     1381 tgccacacgg atgccgccga tcgaccacga ctgctggacg ttcgccttca agggcagcgg
     1441 tatcggtctg tgggattgga atgtcgccat aaacaaagtc attttctcag acggatggaa
     1501 agacctgttc ggatatgcac aggacgaagt cagcgacaat ttcaatgaat ggtcctctct
     1561 catccatccg gaggatcgtt caacggtcat agccaacatt cagcgataca ttcagggcga
     1621 gattccggta ttgacgagtg aacaccggat acgatgcaag gacgggtcgt acaaatgggt
     1681 gttcgaacga gggaagatcg tttcgcggac caaggacggc aaccccctcc gcgtcctcgg
     1741 cattcatacg gacattaccg agcgcaaaca gctggcacga cgactggcca tccagcatga
     1801 ggtggccaac gtgctggcag gcgcttccgg actggataat gcgatttctg cgatcctgca
     1861 accggtttgt caaaccttgg gctggaacga ggggttactg tgggtggtcg acgactcgac
     1921 tcaaagactg cgctgccatt cgatttggac tgcttcgggc accgcatcgg agcgctacgg
     1981 cgccgcgagc cgagagttga cgtttcagtg cggcgtcggg ctaccgggtc gggtttgggc
     2041 cgacgcaaag ccggtgtgga tttcagacgt cacccgtgat gagaattttc cacgagcggc
     2101 attggccgag caggtgggtt ttcatacggc tgcagccttt cctgtcagac tggccgatcg
     2161 tgtctatgcg atactggagt ttttccacca tgagatcctg ccggcagatt ccccgctcct
     2221 gaccaccttc caagccgtgg ccgaccagct cagccagttc tgcgcgcgta agcaggcgga
     2281 ggagcggttg cgtcaaaccc agttcgctat ggaccaggcg gtggatgcaa tttactgggt
     2341 cgatcctcaa gccaggatcc tctatgcaaa tgaggccgcg agcttcatgg tgggctactc
     2401 aagggaagaa ctcctcggca tgacggttca tgacctcaat ccggactttc ctgtttctag
     2461 gtggccgggc ttctgggagg agatgcggcg aaacaagacg atgtcgatcg aaacccacca
     2521 ccgcgccaag gatgggcggc tcgttccgat cgacattcga gtcagtttcc tcgcctatga
     2581 aggacaggaa ttccactgtg cctttgttcg tgacatcacc gaacgcaagc gggcgaatga
     2641 agctctcgaa caaatcatgc accgctacaa agacctgatc aattcgatca acggcatcgt
     2701 gtgggaagcc gacgcctcga cgacgcagtt taccttcatg agcccacaag ccgagaccat
     2761 cctcggctat cccgtcgaac aatggctctc ctcgcccaat ttttgggtgg accacatgca
     2821 tcctgaggac cgggattggg cgccgcagta ctgtcttgag gaggtccgca agcatcgcgc
     2881 ccacaccttc gaataccgca tgctggccgc agacggccgc atcgtctgga ttcgggatct
     2941 cgtatccgtg ctcattgaga atggacgggt taccaagctg cgtggcatca tggaagatat
     3001 caccgaccgc aagcggatcg aagattcctt acgtgacagc gaggagcggt ttcgtacgtt
     3061 cctcgatcat gcgccgaacg ccatgtttat gaaaacgaat gacgggcact atttcttcac
     3121 gaatcagtgc ttcgaacagc tatgtcacag cgatcgagag catgttgtag gtaaaacgga
     3181 tgtggagttg ttcccgaggg aaacggccga acagttccga gccaacgacc gccaggtaat
     3241 cgaaacagga aaagccattg aattcgagga gaccaacgac ctgcatacga atattgtagt
     3301 gaaattcccg gtgagagatc gcttaggaca gatctatgcg acgggtggca ttgttacgga
     3361 catcactgct cgcaagcaga tggagttcaa gctgcaacaa ttcgcggccg aaatggagcg
     3421 acagaacctt gagctggcag aggcacacat gcaagcgctg gcggccacga aggccaagag
     3481 cgagttcctc gcgtcgatga gtcatgagat tcggacgccg atgaacggcg tgattggtat
     3541 gaccggcctg ctgcttgata cgccgttgac cctggaacaa cgcgaatatg ctgaaaccgt
     3601 ccggctttcc ggcgagcatc tgttggacat catcaatgag atcctggact tctctaaaat
     3661 cgaggccggc aagcttgatc tggaagaact caatttcgac ttgcacgcga caatggagga
     3721 ggccgtcggc ctgctcggtg agcgggccta tgccaagggc cttgaactga cttatctcgt
     3781 gcaggcgggt gtgccgaccg cgctgcgatg ggaccctggc cgtctgcggc aaatcttggt
     3841 gaatttgatc ggcaatgcca tcaaattcac ggaacagggc gaagtcgtgg tcactgtgag
     3901 catggagggc aattccagcg aaacgacagc tgcaagcgcg agttcgtcat accggacgtt
     3961 acggtttgag gtgtcggaca ccggagtcgg catcacgccg gagcagcaag cgaaattgtt
     4021 tcagccattt actcaagccg acggctcctc gacgaggaag tatggaggca caggcctggg
     4081 cctcgccatc tgcaagcagc tcgtggagat gatgggaggg agaatcggtg tggacagcaa
     4141 ggtcggggaa ggcagcgtct tttggctcac ggtacggttt ccactccaac cggaaggggc
     4201 tcagccggtc gcgaccattc cggcatcact acggggtcgt agaattttga tcgtggacga
     4261 tcatgccacg aaccggcgag tgcttgagca gagtctacga gggctagggg tggtgtatga
     4321 aagcgcggag aatgggtatc aagcgctgga atgtttgcgc aacgcggccg gtcggcagaa
     4381 tcatttcgat gtggccattt tggatatgca gatgccgggc atggacggtc tggaattggc
     4441 ccaccggatc aagtcggaat cggcgatcag tgcgacacgt ctggtacttc tgacgtcggc
     4501 cggccagcgc ggcgatgcca aagcagctca aagcgccggg atcgccgcct atctgacgaa
     4561 accgatccgg cagtcgctct tatacgagtg tctcggcctt gttctgggaa gtgtccccaa
     4621 ttctgttgct ccaacccctc agaccactgc cacgatcatt acccgccata gcctgtcgga
     4681 agcgcagacc cgatccagac cgcttgtcct tctggttgag gacaatccgg ttaatcaaaa
     4741 ggttgcggcg aatatgatcg agaagcttgg gtatcgggtc aacgttgcgg ccaccgggcg
     4801 agaagccgtc gattcactcg cctgtattcc ctatgcgctg gtgttcatgg attgccaaat
     4861 gcctgaaatg gatgggtttg aagcgacaag agtgatcagg aaacaggagg agagtctgcg
     4921 acaggttggt ggcaggcagt cccacctgcc tatcatcgcc atgactgcca acgcaatgca
     4981 ggaggatcgt gaccggtgcc tggcagtcgg gatggatgac ttcctcagca agccggttac
     5041 gagcaaatcc ctggcagcgg tcctagatcg ttggctgccg cacgaccagg ctcctgcgga
     5101 gacagaatca aaagcagcct agtctcccct ccgagcatct tccaaccgac agtctgaagt
     5161 cgtacaatcc gtcttcgact tgagtctgtg cgtatcgatg gcacatcata aacaatagcc
     5221 gaccataaac agcgatcaac tctccagatg gcttgcttga tgcggttcca tatcaacgct
     5281 gtttccccat ttgaagcacg atgaagccac gtgtcatcgt caccggagcg gcgggactga
     5341 ttgggcaata ctgtgtgaag tccgcctcac gttgggctcc ggactgggac gtgcggggtc
     5401 tcagccgcac cgaccttgat ctcacggatc gcgccgctgc agaacaggct tggcggcatc
     5461 tcaaacccga cgcagtcatc cactacgcag ccttaagtcg gacgaaagat tgcgagcaga
     5521 atccccagct cgctcgccgt atcaatataa acgccacggc tgatctggct cgactctcca
     5581 aggacattcc ctttatcttt ttgtcgagcg gcgaagtctt cgatggcgaa cgagcctggt
     5641 atcgggaagg agacgcaccg aacccgatca atttctatgg caagaccaag cttgaggcag
     5701 agcgcctggt gttgcagaat cctcgtcaca ccgtggttcg catcgtcctg acggccggta
     5761 cgtcgcagaa tggcgatcgg agttttgtcg aagatatgtc ccgggcggcg aggagcggca
     5821 agagcgtgac tctctatgct gacgagtatc gttgtccgtt gccggccgga gccattgcgc
     5881 gtgcgatctg ggagttagtc ggcaaggacg agtccggtct ctttcacctt gggggacatg
     5941 aacggttatc acgctgggag gtgggccagg cgctgctgcc ctggtatccg gaattgcaag
     6001 gccgtctcgt gaaaggatgt gcccatgatc atcccggcgc acctcgaccc gccgatctct
     6061 cactgaactg tgagaaaatt caggcgtgct tgtcgtttcc cttaccgggt tttcgaagtt
     6121 ggttgaacga ccgggtgcat caaggtgtcg atgtgtggga ctatgagcac agtctgtcgt
     6181 gaggcataga agataatgga accagagaac gggatatttg catcggatcc gttgccgcat
     6241 gtcttggcgg cgtatctttt catgacgata gtcatggggg cattgtgggc cgtgcagcgg
     6301 aaaaccagga atgccgcgat tggagatgtc ggatggtgtt ttggacttat tctggtcgtg
     6361 ctgtggtatg ccacacaggc aagcggcggt ttcgcgcgag tctttctgcc cgtgatgctg
     6421 gtgacactct atgcgggacg gctgggtctc cacattctgt tcaatcgcgt gatcggcaaa
     6481 gaagaggatg cccggtatcg ccggctgcgg gaggaatggg gcgagtcgga gtcggccaaa
     6541 atgctggcgt actttctcct acaagcgctg gccgtggcgg tcttttcgct cccgtttctg
     6601 gtgctgatgt ggaatcctcg tcctccctcc gcgttggttg aactcattgg tttgatgatc
     6661 tggggggtgg ctgtggccgg tgaggccaga gccgacagac aacttgccca cttccgggcc
     6721 gacccaagaa atcagggccg cgtctgtcga gagggcctct ggaaatactc acgccatccc
     6781 aactattttt ttgaatggct gcactggtgc tcatatgttg tgatgacatt gggagcaccg
     6841 ggctgggtct ttacctggat cgggccgatc ggaatggggt tggcgctgct caaactgacg
     6901 ggtattccac gaacagaagc gcatgccctt tcgagccgcg gagaggagta taaagcctat
     6961 caagcgacaa ccaatgcctt tttcccttgg tttcctcggc caaggccgga catggctact
     7021 ccttcataac gcagacgacc agccggagcc cgcatgcaat cgtaagcgtc cttcacgact
     7081 gccggcatag agtcggctgc gtctatttcc tgctgtccag atcggcaagt cgtgcgcgag
     7141 cgatctcggc cactttggtg gatgggtaga gatttaccag ctcttggtag agctgcttgg
     7201 catgagggac attaccttgg ttttcttcga aggcggccgt ttcaaacagc tctttggcct
     7261 tctgatcgga gcagccgagc agcatcaaac agaagacaca gagtacaagt cgtgcggtta
     7321 tcatggtgat cctttccagg catctctccg tcgggacacc ttcgtgttcg catgagtaga
     7381 tcctgatcca agagaatcga ttccgtagcg tggggcggaa atcccttcgc ttcgacagtg
     7441 tgggcagcgg ctcggtcgtg tcaatcttgt gcggtcgcga aacataaatc cacaatccaa
     7501 acacatggat ggttcaagga taaaccgtcg tgaacggtcc cgagccaccg tttttaccac
     7561 atgagccaag tgttcctcca cctgtcgttc cggaataccg agcatccgag ccagttgatc
     7621 ggttgtgagt cgagtggtgg tcaaaagatc aatgatgcgc tgacggagcg tgcgttcagg
     7681 aggaagcatg cccgtatggt aaggactagg tcacagaaaa gccagatgat ctgggcgggt
     7741 cttctacgaa tcgtcttaag tcttgcatac ttaaaggttg caccgacacc tccaatctgt
     7801 caaaatccgt tcccgcatca cttgtcacat caccattaat gatccactgg gccggcctgt
     7861 cgctcgtatc cttgaaacac tagggttcta gcccagcgcg ctatagacgg aggtttgcgg
     7921 caatgaaatt cctcgcagtc catcccggtc cactcatgta caccaagatt tatctacgtc
     7981 tggaaccgct cggcctcgag atggtggcgc aggcgtgccg ccaagcggga cacgatgtcc
     8041 gtttgatcga cttgcaggag gatacgtgga aagactacga ggcgcttatc cggacctgga
     8101 agcccgatgc cgttgcgttc ggctgtaatt acctggcgaa tgtcccggag atcgtggatc
     8161 tggcgaagct gaccaagcag gaactgccga actcgttcgt ctttgtcgga
//
LOCUS       DATMTI010000501         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_528909, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000501 DATMTI010000000
VERSION     DATMTI010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528909"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1800
                     /gene="recJ"
                     /locus_tag="VN039_08080"
     CDS             100..1800
                     /gene="recJ"
                     /locus_tag="VN039_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HWV45960.1"
                     /translation="MKSKLWVVRQVDPVQRGLLSQALSISSATASLLLNRGVTNVDQA
                     TAWMSHIRTHDPFLIPDMESAVDRLYQAMQRREVVCFYGDYDVDGMSATSIYLSFFRG
                     LGAEIRAYVPHRLREGYGLNESAVRTLAKEGVSLLVTSDCGTTSHREISLAGQLGIDV
                     VVTDHHQSDEEMPPALAVMNPHRPEARYPFHGLCSGGLAYKVAQAYDRKYGSGSVPLE
                     SLLDLVALATIADVVPLQDENRLFVREGLARISRGARCGIRALKHVAGISRECTTETI
                     AFKLGPRLNAAGRLDEAIKGVRLLTTESEREAKELAEDLERLNRARRDLEAEILQEAL
                     AQVEAREWPGGIVLYARGWHLGVVGIVAARIMERFHRPTVVIAVNEQGIGKGSARTVP
                     GFDLYQALTACRDLLMAFGGHPSAAGITIEEARLPEFSDRFSAIAGTWIRETQSVPML
                     HVDSEVLLNEVTLQLIREIGTLHPFGAGNPEPTFAVKGLDVLNARVVGEKHLKMTVRQ
                     GGSVPFDSIGFGMNSLEGHGLSLKTPIDVAFTPELNHWNGYDRIQLRIRDIRAAGRES
                     "
     gene            1804..>3570
                     /locus_tag="VN039_08085"
     CDS             1804..>3570
                     /locus_tag="VN039_08085"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWV45961.1"
                     /translation="MYETVTSIDQLLDRLQDYQPDADLGMVRKAYEFSARAHEGQVRR
                     SGEPYVKHPVAVAGVLTSLKTDVTAVVAGLLHDTLEDTVATADELQREFGKEVVHLVD
                     GVTKIGKITFKSSEEKQAENFRKMVLSMADDIRVVIIKLADRLHNMRTLEHLSEKRRQ
                     EIAQETLEIYAPLANRIGIGWVKNELEDLCLKHLKPDVYETLRVCVAKRDEDRRQYIQ
                     EVQALVESALKENGLVGAVYGRPKHLYGIYQKMKKQSISFEEVYDLTALRIITDTKMN
                     CYALLGVIHSLWRPLPGRFKDYIAIPKSNLYQSLHTTVVGPKGEHVEFQIRTEEMHRV
                     AEYGIAAHWKYKEQGRVHDKDGKAFGWLRQFIEWQKDLPDNRQFMDSVKLELFHDVVY
                     VFTPKGIVKELPKGATPVDFAYAIHTEVGDHCVGAKVNGKIVPLKHEVSSGDTIEILT
                     SPNQTPHKDWLKFVRTSRAKTKIKHWIKAEEQKRSLEIGWRLLETEIRRHGMAPSQVL
                     KSDALLEMAKREGYATIDELAAAVGFGHLATAQLIGRLTAPISAGAPTQSQTASALKV
                     ASTRGEEPGVQVKGGRDLLMQLS"
BASE COUNT          827 a          874 c         1085 g          784 t
ORIGIN      
        1 tggtttgaca aagaagaagc gacttattac agtcttcgtc gttcctctaa gactcatggt
       61 ctaagttgtg acacggccca gtttcttaag gaggcggagg tgaagtctaa gctctgggtg
      121 gttcgccagg tcgatccagt ccaacgaggc ctcttatcac aagccttatc tatctcatcg
      181 gctactgcct cgttgttgct caatcggggt gtcacgaacg tcgatcaggc gacggcctgg
      241 atgtcccata tccgaaccca tgatcctttc ttgattcctg atatggaatc ggcggttgac
      301 cgtctgtatc aggccatgca acggcgcgag gtcgtatgtt tttatggcga ttacgatgtg
      361 gacggcatgt ccgccacgag tatttacctt tcattcttcc gtgggctcgg tgccgaaatc
      421 cgagcctatg ttcctcatcg cctgcgtgaa ggatatggac tcaatgagag tgctgttcgg
      481 accttggcga aggagggtgt ctccttgctg gtgacttccg actgcgggac cacgtcgcac
      541 cgtgagatca gccttgccgg tcaattgggg atcgatgtcg tcgtgaccga ccaccatcaa
      601 agcgacgagg agatgccgcc ggctttggcg gtgatgaatc cgcatcggcc cgaagctcgg
      661 tatccctttc acgggctttg ttccggtggg ctggcgtaca aggtcgctca ggcctatgac
      721 aggaagtacg gctccggttc ggtgccactg gagtcgctat tggatttagt ggcacttgca
      781 acaattgccg acgtggttcc gctacaagac gaaaatcgac tattcgttcg agaaggtctc
      841 gctcggattt cacgaggggc gcgctgcggc atacgtgcct tgaagcatgt cgccggtatc
      901 agtcgtgaat gcacgacgga aaccatcgcg tttaaactcg gacctcgact caatgccgca
      961 gggcgattag atgaggctat taaaggggtc agacttctga cgacggaatc tgaacgggaa
     1021 gccaaagagt tggctgagga tttggagcgg ttgaatcggg cccgccgtga tctcgaggct
     1081 gagatcctac aggaagcgct agctcaggtg gaagcacggg agtggccggg tggcatcgta
     1141 ctgtatgcac gaggatggca cctcggggtc gtcggtatcg tggccgcccg tatcatggag
     1201 cggttccatc gtccgacggt cgtcatcgct gtaaatgaac agggtatcgg aaaagggtct
     1261 gctcggaccg tgcccgggtt cgacttatat caggcattga cagcctgtcg agacctgctc
     1321 atggcattcg gtggccatcc cagcgcggcc gggattacaa ttgaggaggc gcggttgccg
     1381 gagttttccg accggttttc cgccatcgcc ggaacatgga ttcgagagac tcaaagcgtt
     1441 cccatgctgc atgtggattc agaagttctg ctgaacgagg tcacgttgca gctgattcgg
     1501 gaaatcggga cgttgcatcc attcggggct ggaaatccgg agccgacgtt cgccgtcaag
     1561 ggtctggacg tgctcaatgc tcgagtggtc ggagagaagc atctgaagat gacggtccgc
     1621 caaggagggt cggtaccgtt tgatagtatc ggattcggga tgaattcgtt ggagggccat
     1681 ggattgtctc tcaagacacc gatcgacgtc gcgtttacgc cggaactgaa tcactggaac
     1741 ggttatgatc ggattcaact gcgtatccga gatatacggg cggcaggccg tgagtcatag
     1801 aagatgtacg aaaccgtgac gagcatcgat caattgttgg atcgtctgca agactaccag
     1861 cctgacgctg atctcggcat ggtacggaag gcctatgagt tttccgcgag ggcgcatgag
     1921 ggacaagtgc gtcgatcagg agagccctat gtgaagcatc cggtggcggt ggccggagtg
     1981 ctgacgtctt tgaaaaccga cgtcaccgcg gttgtggccg ggcttttaca cgacacgctg
     2041 gaagatacgg tcgcgacggc cgatgagctt cagcgggagt tcgggaagga agtcgttcat
     2101 ctcgtcgatg gggtgacaaa gatcgggaag atcaccttta agagttcgga agaaaaacag
     2161 gctgaaaatt tccgcaagat ggtgctctcg atggcggacg atattcgcgt cgtgatcatc
     2221 aaattggccg atcggctcca caatatgcga actcttgaac atctcagtga gaaaagaagg
     2281 caagaaattg cacaagagac gttggaaatc tatgcgcctt tggcgaatcg catcggaatc
     2341 ggatgggtga agaacgaact ggaggatttg tgtctgaaac atctcaagcc cgatgtctat
     2401 gagaccttac gggtgtgcgt ggcgaagcgt gatgaagatc gccggcagta tatccaggag
     2461 gtgcaagcgc tcgtcgagag tgctctgaaa gagaatgggc ttgtcggagc agtgtacggg
     2521 aggccgaaac acctctacgg tatctatcaa aaaatgaaga agcaatcgat ctccttcgag
     2581 gaggtctacg atcttaccgc cttgcggatc attaccgata ccaagatgaa ctgctacgcg
     2641 ctgttgggcg tcatccattc gttgtggcga ccgctgccgg gccgcttcaa ggattacatc
     2701 gcgatcccca aatcgaatct ctatcaatct ctccatacaa ccgtagtcgg accgaagggt
     2761 gagcacgtag agtttcagat ccgcactgaa gagatgcatc gcgtggccga gtatgggatc
     2821 gccgcgcatt ggaagtataa agaacagggg agggtgcatg ataaagacgg caaggctttc
     2881 ggatggctga ggcagttcat cgaatggcaa aaagatctgc cggacaatcg gcaattcatg
     2941 gattccgtga aacttgagtt gtttcacgac gtggtctatg tctttacccc caagggaatt
     3001 gtcaaagaac tgcctaaagg agccacgccg gtagactttg cctatgcgat tcacacggag
     3061 gtcggcgacc attgcgtcgg ggcgaaagtc aacgggaaga tcgttccgct caagcatgag
     3121 gtgtcgagcg gcgacaccat cgaaattctg acatccccga accagacccc gcacaaagat
     3181 tggctcaagt tcgtacgcac atcacgggcg aaaacaaaga ttaaacactg gatcaaggcg
     3241 gaggaacaga aacgaagctt ggaaatcggt tggcgtttgc tcgaaacaga gattcgtcgc
     3301 catggcatgg ctccgtcgca agtcctcaag tcggatgccc tgctcgaaat ggccaagcgg
     3361 gaaggctatg caacgattga cgagcttgca gcggccgtgg gatttggtca cctcgcaacg
     3421 gctcaactca ttggacggtt gacggctccg atctcagccg gtgctccgac tcagtcccaa
     3481 actgccagtg ctctcaaagt cgccagcacg agaggagagg agccgggcgt ccaagtgaag
     3541 ggggggcgcg atctgttgat gcaactgtcg
//
LOCUS       DATMTI010000502         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_232711, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000502 DATMTI010000000
VERSION     DATMTI010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232711"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VN039_08090"
     CDS             <1..534
                     /locus_tag="VN039_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV45962.1"
                     /translation="FYFINLIFIGRFIEALPSIVYHALMSTGLFIQSWRLYKNQSIES
                     LSDAARIPTVVLEQIEADQTDPTTATIEALASALRVPPSWLFDDPRSFEYLFADSAES
                     EEPDRSQLDPVTDRILAGSHTDRSLYVLLTTLIQAADPKLLRAAEMSLRSLVKQARHA
                     TVPWQQRPSGHFEPPSD"
     gene            complement(573..>2969)
                     /locus_tag="VN039_08095"
     CDS             complement(573..>2969)
                     /locus_tag="VN039_08095"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWV45963.1"
                     /translation="GIDPADLSLDLGSLLMRIGTDGDALHLSNFDPNNVLGPRTIETF
                     RFADGTVLPYDQLVQRGFDLTGTDGNDSIAGTNLVDRITALAGDDTLAGGRGNDVLTG
                     GSGDDTYLFNIGDGSDTIDDTVLLGAGNRIRFGTGISQTDLIFTQDEAAHTLTIHVGT
                     GGTDRLVLADFDPRGMNGSLVVSALEFADGNVVNLIDLYPPNHAPMVTVPTADQTAAE
                     DSPFFFTIPSTTFTDPDQFHGDELSYEATLATGSPLPAWLGFDPITSTFSGTPGAGDA
                     GVLQLAVTATDQGHLSTTDFFTLSISGPLPQTLVGTLEDDRLTSGRGDDTLRGLAGND
                     QLNGGLGHDLLDGGSGTDTMRGGIGNDTYVVDVAGDVVTELVDEGTDTVQTSLLTYTL
                     GVNVENLALTGTGPSTGIGNALNNQLTGNNGANLLDGKAGADTMVGGMGNDVYVVDQT
                     DDVVTEATNEGLDTIFSSLTYSLGANVENLVLTGSAAIDGTGNDFNNTLTGNSAANVL
                     AGLEGNDTYVISGGDTVVEAADNGTDTVVSSRSYTLAANVENLTLVGFNTISGTGNSL
                     DNVLNGLLNLAGNTLVGEAGNDTYIIGSGDKVVEAAGEGTDTVQTSLSHVLGANVENL
                     TLTGVSAVNGTGNSLNNTLTGNGASNVLTGAGGNDMFRGSAGNDTLDGGNGNDTYLFD
                     RGDGQDFVRDNSGTADKLLYDSGIDPLDLVISHQANDLRLAIHGSTDSVTIRNWYTGS
                     SNRTEMIQAGNGQTLLNTQVNQLIQAMAAFTQQTGLTWDQAIDQRPQDVQTVLAASWQ
                     "
BASE COUNT          616 a          893 c          778 g          682 t
ORIGIN      
        1 ttttatttta taaatctcat tttcatcgga cgatttattg aagctctccc ctccattgtg
       61 tatcatgccc tcatgtcgac agggctattt atccagagct ggaggctcta caaaaaccaa
      121 tccatcgagt cgttatcaga cgcggcacga attcccactg tggtcctaga acagatcgaa
      181 gcggatcaga cggaccccac gacggctaca attgaggccc ttgcgtccgc acttcgcgtg
      241 cctccgtctt ggctgttcga cgacccccgg tccttcgaat atttgttcgc agactcagcg
      301 gagagtgagg agccggacag gtctcagctt gatccggtga cggatcgaat cttggccgga
      361 tctcacaccg atcgatcact ctatgtcctt ttgacgacgc tcatacaagc cgctgatcca
      421 aaactcttgc gggccgccga aatgagccta cgcagcttag tgaagcaagc tcgccacgcg
      481 accgtaccct ggcaacaacg cccctccggt cacttcgagc cccccagcga ttagaccggt
      541 cgtctgaacc ctgaaccggt cctgctcatc atctattgcc agctcgccgc taacacggtc
      601 tgcacatcct gcggccgctg atcgatcgct tgatcccagg tgagcccggt ctgctgtgta
      661 aaagccgcca tcgcttggat caactgattg acttgcgtgt tgagcaacgt ttgtccattg
      721 ccagcttgga tcatttccgt tcgatttgat gaaccggtat accagttccg gatcgtgacc
      781 gaatcggtcg agccatggat ggccaatcgc agatcgttcg cttggtgact gatcacgaga
      841 tcaaggggat cgatccccga gtcatagagc aacttgtcag ccgtgccgct attgtctcgt
      901 acgaaatctt gtccgtcgcc gcggtcgaaa agataggtat catttccatt gccgccgtcg
      961 agcgtgtcgt tgcccgcact accgcggaac atatcgttgc cacctgcccc agtcaaaaca
     1021 ttgctcgcac cgttgccggt gagcgtatta ttgagtgaat tgcccgttcc attgacggca
     1081 ctgactcctg tcaatgtgag attttccaca ttggcaccga ggacatggga caggctggtc
     1141 tgaactgtat ccgttccctc ccccgcagct tccaccacct tgtcgcccga accaatgatg
     1201 tacgtatcgt tgccggcttc cccgactaga gtattgcccg caagattgag cagcccattg
     1261 agcacattgt cgaggctgtt gccggttcca ctgatcgtgt tgaaaccgac tagggtgagg
     1321 ttttccacat tggccgcgag tgtgtaggaa cgactactga ccaccgtgtc cgtgccgttg
     1381 tccgccgctt cgacgaccgt atcaccgccg ctgatcacat aggtgtcgtt gccctctaac
     1441 cccgctaaca cgttggccgc actattgccc gtaagggtgt tgttgaaatc gtttccggtc
     1501 ccgtcaatcg ccgccgagcc cgtcaagacg agattttcta catttgcccc tagactatag
     1561 gtcagggagc tgaagatcgt gtcgaggcct tcgtttgtcg cttcagtcac gacatcatct
     1621 gtctgatcca ccacatacac atcattcccc ataccaccga ccatcgtgtc ggctccggcc
     1681 ttgccgtcca gcaagttcgc tccattgttg ccggtcaact gattgtttag agcattgccg
     1741 ataccggtgc tcggccccgt acctgtcagc gccaggttct cgacattcac acccaacgtg
     1801 taggtgagaa gcgaggtctg taccgtatcc gtaccttcgt caaccaattc agtcacgaca
     1861 tcgccggcga catccacgac gtaggtatcg ttgccgatcc ctccccgcat cgtgtctgtg
     1921 ccggatccgc catcgagcag atcgtggccc agtccgccat ttaactgatc gttccctgcc
     1981 agcccgcgca atgtatcgtc gcctcggcca ctagtcagcc tatcgtcttc cagcgtgccc
     2041 acaagtgttt gaggcagagg accggagata gagagtgtga agaaatcggt cgtgctgaga
     2101 tggccctggt ctgtggcggt gacggcgagc tgcagtacac cggcatcgcc ggcacccggc
     2161 gtaccgctga acgtgctggt gatcggatcg aaacccagcc atgccggcaa tgggctgccg
     2221 gtcgccagcg tcgcttcata ggataactca tcgccgtgaa actgatctgg atcggtgaag
     2281 gtggtactgg gaatcgtaaa aaagaacggc gagtcttcgg cggcggtctg gtctgccgtt
     2341 ggaacagtca ccatcggggc atgattcgga ggatagaggt caatcaggtt caccacgttc
     2401 ccgtcggcaa actccagtgc cgatactacc agagaaccgt tcatgccacg gggatcaaaa
     2461 tcggcaagca ccaggcgatc ggtcccgccg gtcccgacgt gaatggtcag ggtatgcgcg
     2521 gcctcgtcct gcgtgaagat caagtcggtt tgactgattc cggtcccgaa tcgaattcgg
     2581 ttccctgcgc cgagcagcac cgtatcatca atcgtgtcgc tcccgtctcc gatgttgaag
     2641 aggtaggtat cgtcgccgga accgcccgtc agtacatcgt tgccgcgccc accggctaat
     2701 gtatcgtccc ctgcgagagc cgtgattcga tcaactagat tcgtgcctgc gatagaatca
     2761 ttgccgtcgg tccctgtcag atcaaaccca cgttggacaa gttggtcata gggcaggacg
     2821 gtgccatcgg caaatcgaaa ggtttcaatc gtgcgcggcc ccaacacatt gtttggatcg
     2881 aaattggaca gatgaagagc atcgccgtcg gtaccgatac gcatcagcag cgaacccaaa
     2941 tccaggctga gatcagcagg atcgatccc
//
LOCUS       DATMTI010000503         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_826079, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000503 DATMTI010000000
VERSION     DATMTI010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826079"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VN039_08100"
     CDS             complement(<1..1156)
                     /locus_tag="VN039_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45964.1"
                     /translation="MTQPNNVVQLLNVGDTVPLGKILANIVLMGVATDPTTPNMFSAT
                     LEAFGDQGVLTVPVLQGPPGPQGQPQFALRFQADNLTGPSQLPNNLTNTTADIGKFWI
                     FATTDSNGNIVSTIAYIWYGTEYRQLPMGSQGPPGPYPVITPTVNLLDPDLTSYIEVS
                     GPTSNPSWELHLAVPAGPTGPAASLATAPDVNLTQPPQLGQVLGFNGQYTLEGAPIFQ
                     PMWVGDILPRPYTIPESAFQSYSGITSGSQTICTWAAPAQQWPWQPWVSGQIDVIGVD
                     LSLLPELSVEVRINDPGSGPIVAYGNSNSSGSITLTQHTSSKSNPSAAMSPTNGYAQV
                     AAGSSATLYVNLLNTGLATAYRFSSDNAQLSMLALPVGTERAFPTTYFGTL"
     gene            complement(1153..1416)
                     /locus_tag="VN039_08105"
     CDS             complement(1153..1416)
                     /locus_tag="VN039_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45965.1"
                     /translation="MTKDIKRVDLSKLTPEQKQAVAQFYRDCKQAAPHVKQMQDTMHT
                     LFQAWLQSPQWTVHEGTKPDGAHQDVWHVKPEINYVDRIEGVS"
BASE COUNT          306 a          418 c          479 g          321 t
ORIGIN      
        1 tgagtgtgcc gaaatatgtt gtcgggaaag ctcgctccgt acccaccggc aaagccagca
       61 tgctcagctg ggcgttgtct gaggaaaatc tgtaggccgt cgccagtccg gtgtttaaca
      121 agttcacgta cagggtggcg ctgctacctg ccgcgacttg cgcgtagccg ttggtcgggg
      181 acattgcggc ggacgggttc gacttactgg acgtgtgctg ggtaagggtg atcgaccctg
      241 acgagttcga gttcccgtac gccacaatgg gacccgaacc aggatcatta atcctgactt
      301 caaccgacaa ctcaggaaga agagatagat cgacgccgat gacgtcgatt tgtcctgaca
      361 cccatggctg ccacggccac tgctgcgccg gagccgccca cgtgcaaatc gtctgcgacc
      421 cactcgtgat acccgaatac gactgaaacg ccgactcggg aatcgtgtag ggcctgggga
      481 gaatatcccc gacccacatg ggttggaaga tcggcgcacc ctccaacgtg tactgcccgt
      541 tgaaccctaa aacctgaccc aactgtgggg gttgggtcag gttcacatcc ggtgccgtcg
      601 cgagggacgc tgcgggaccg gtggggccgg cggggaccgc cagatgcaac tcccacgacg
      661 ggttagacgt cggccccgac acctcaatat acgaagtcaa atcagggtcg agaagattca
      721 cggtcggggt gatcaccgga tacggcccag gaggaccctg ggaccccatc ggcagctggc
      781 ggtattcggt gccgtaccaa atgtaggcga tcgtggaaac aatgttgccg ttggagtcgg
      841 tggtggcgaa gatccagaac ttcccgatat ctgcggtggt gttcgtcagg ttgttcggca
      901 actgggatgg gccggtgagg ttgtcggcct gaaacctcaa agcgaactgc ggctgcccct
      961 gcggacccgg aggaccctgc aacacaggca cggtgaggac gccttggtcg ccgaacgctt
     1021 ccagagtcgc cgagaacatg ttcggggtgg tggggtcggt cgcgacaccc attagcacaa
     1081 tgttggcgag gatcttcccc aacggcacag tgtcgccgac gttgaggagc tgcacaacat
     1141 tgttgggttg tgtcacgaca ctccttcaat ccggtcgacg tagttgattt cgggtttcac
     1201 atgccacaca tcttggtgcg cgccatcggg tttcgtccct tcatgaacag tccattgcgg
     1261 ggattgcagc catgcctgga agagggtgtg catggtgtcc tgcatctgtt tcacgtgggg
     1321 tgcggcttgt ttgcagtccc gataaaactg agccacagct tgtttctgtt cgggggtgag
     1381 cttcgaaaga tcaacgcgct taatgtcttt ggtcaacggt ctacccgaat atcgtgtttt
     1441 cgccgaggaa cgcctggaac agcccgtaga acgcgttcaa cgaccggagg gttctggcga
     1501 tggggtcttg tttgtcccgg tcat
//
LOCUS       DATMTI010000504         6173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_359239, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000504 DATMTI010000000
VERSION     DATMTI010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359239"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VN039_08110"
     CDS             <1..747
                     /locus_tag="VN039_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWV45966.1"
                     /translation="SDRKRRKMPVKEELDHNQLLAHLPIDEKRALGTQLQLVSLRTKE
                     EITGVGGPLRHVHFPLTAAISLMNMQEGGQTVEVAVIGKEGCTCFHVLDGLKASPSRI
                     VVQIGGSAFRMLSAALDDIIKDLPVFAQLVRRYSAVLFRHAVISVGCSQFHSVEQRLA
                     RWLLAHNNRTGATTFPFTHEFLAEQLGSQRATITQGLADLQNKGLLTSGYGTVELLNI
                     PKLQIVACECFGLSALAIDEYLTDIEMYQR"
     gene            complement(1071..1193)
                     /locus_tag="VN039_08115"
     CDS             complement(1071..1193)
                     /locus_tag="VN039_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45967.1"
                     /translation="MIFKLWQTFVNAVAVVMEPQRPGMGWGKGWIGYGHTGGSR"
     gene            complement(1183..2331)
                     /locus_tag="VN039_08120"
     CDS             complement(1183..2331)
                     /locus_tag="VN039_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWV45968.1"
                     /translation="MHSILRLVRIGIPAVGILILLAGVTLVLSLMVPFEVWRTGELPT
                     EPIQVRPAEPFGTSPVRVWIDTDAACGKGRRTDPDDCLAILLLAQQDRIHLVGVSTIF
                     GNAPLDGTDRTTRDLIAFLETGRSHAIPVYRRASDPIKQEEMIESIRPREAHEALRKA
                     LQEEPLAIVALGPLTNVALALKDRPQLQANVTRLVAVMGRRKGHVFHPVEGGTAHSFL
                     GHGPIFRDFNLTEDEQAAAVVLAMGLPLTLIPYEAARDILLDASCLDKMEARGGPAAW
                     VAHHARPWLSYWQEDIGIDAFYPFDLVAASYVVQPSLLYCANVPIGIEDDTWLFGWLG
                     YRGLFVEPKHETGSRPLVSGSALYCPEVSKHLQTWLMDQLTNEHEGDL"
     gene            complement(2388..4022)
                     /locus_tag="VN039_08125"
     CDS             complement(2388..4022)
                     /locus_tag="VN039_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWV45969.1"
                     /translation="MICIKRIEGTVPINREVLIVGGGLNGLTTALLLAHHGVECLVVE
                     RHPNTSIQYKFAGISPRSMEIFRSLGIEEEIRAKRTGDQQAGGVARGKNLADPAIEWM
                     RPHAWPDINGLSPTPPATCDQHVLEPILRAHAERLGADVRFNTELVSFVQDGEVRALI
                     RNRETGEEQEVQAAYLIGADGANGTTREAVGIARNGPGVLQHWMNIIFDTDLPPAVQG
                     KRFTSCFLTEINGTFTPRENGRWLLALQYFPERDESPEDFDDDRCRVLVRRGAGRADV
                     KADLVDARSWEVAALIAGRFREGRVFLVGDSAHLMPPTGAFGGNTGIHDAQNLAWKLA
                     AVLQGRADPALLDSYDRERRPVAETSLEQALTRLQAWFKDPNKRLPPPVPLVDDYDVV
                     FGHLYHTGAVMTEGETAGRAFAKARELSGQPGSRAPHLVIEHYGRRMSTLDLFNKDFV
                     LLTGAAGTAWCEAALAIADRRPYNLQCYRIGSGGDLVDLENRWPVAYGVTESGAVLVR
                     PDGFVAWRSRDCPSQPSRVLRDVFARIGLRIPFETC"
     gene            complement(4019..4384)
                     /locus_tag="VN039_08130"
     CDS             complement(4019..4384)
                     /locus_tag="VN039_08130"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV45970.1"
                     /translation="MPANSPEEICRLFKEHMAEGDLDALLNLYDPQAVFLNQAGEVKN
                     GDGLKQELSTFAAMKTRFDFTIKQTVQTGDIALMHTQWTVSGPQPKKVYAIEVARRQL
                     DGTWRWLIGDPFTVMRAEP"
     gene            complement(4532..4795)
                     /locus_tag="VN039_08135"
     CDS             complement(4532..4795)
                     /locus_tag="VN039_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45971.1"
                     /translation="MWWTAGALLLMLAAHTVYWLMTHPINNFWVKDVATSGAGLAFFS
                     TFANTQTGDWTELRDAWERSHAVRACLHVLALIAIAMATSLFP"
     gene            complement(5048..5563)
                     /locus_tag="VN039_08140"
     CDS             complement(5048..5563)
                     /locus_tag="VN039_08140"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV45972.1"
                     /translation="MTKQPAKNTICLWYDRDAEEAARFYAKTFPDSSVGAVYRAPSDF
                     PSGKKGDVLMVEFTVAGISCIGLNGGPAFKHNEAFSFQIATDDQAETDRYWNAIVGNG
                     GQESECGWCKDKWGVSWQITPRVLMQAHAAGGEEARRAFEAMMTMQKIDVAAIEAARR
                     GSGVKAARAKA"
     gene            complement(5681..6061)
                     /locus_tag="VN039_08145"
     CDS             complement(5681..6061)
                     /locus_tag="VN039_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV45973.1"
                     /translation="MKNKVCFFEIPASDVNKAKTFYETVFDWKVEPEGNEGAMAFTTA
                     SDKEHHPTEPGDINGEFYKRKSKEDHPSVGVETDSIDNTLKAVEKAGGKVVTPKHPIG
                     EWGFMADFADSEGNVMALWEKPKK"
BASE COUNT         1307 a         1800 c         1681 g         1385 t
ORIGIN      
        1 tccgacagaa agcggcgaaa aatgcctgtg aaagaggaat tagatcataa tcaattgctc
       61 gcccatcttc ctatagatga aaaacgcgcg cttggtacac agttacagct cgtgtcccta
      121 cgcaccaaag aagaaatcac cggtgtggga ggccctcttc gtcatgtgca ttttccactg
      181 accgccgcga taagcctcat gaacatgcag gaaggcggcc aaacggtgga ggtcgcggtg
      241 atcgggaagg aaggctgtac gtgttttcac gtcttggatg gtctcaaagc atcgccgtcc
      301 cgcatcgtgg tgcaaatagg aggcagtgca tttcgtatgc tgtccgcagc gctcgatgat
      361 atcatcaaag accttccggt atttgcgcag ctcgtcagac gctatagcgc cgtgttgttt
      421 cgtcacgccg tcatttcagt gggatgcagt cagttccatt ccgtcgaaca acgactggcc
      481 cgctggcttc tcgcgcacaa taatcgaacc ggtgcaacga cattcccctt cacacatgag
      541 ttcctggcag aacagttagg gtctcaacgt gcgacgatca cgcagggact cgccgatcta
      601 caaaacaagg gtttattgac atcaggatat ggcactgttg agctgcttaa tattccgaag
      661 ctgcaaatag tcgcttgtga atgctttggc ctttccgctc tcgccatcga tgaatattta
      721 acggacatcg aaatgtatca gcgataaggc tttgcaggaa gccatgccgc ctgtccttcg
      781 ctcggtctat tcgtcagtga ccctttctac aacctaggca ttcatggaag gatcatttgg
      841 atatcatcaa aacagtcgtc gactactaaa tacgtgaaat ggtgtaaccg tatggaagtt
      901 attgtgtgca gcgttggtcg caggcgcagt agccctgatg gtggaaggcg ttatgatgaa
      961 acactgatcg gcacatcatt cgatcggatt gccgctcaga aggaagcgaa tggaatcata
     1021 ggcaatcagg cttcctagct gcctcaatga ttacgcgcgt tccattttgt tcatcgggaa
     1081 ccgccggtgt gtccgtatcc gatccaccct tttccccatc ccatgccggg acgctgaggt
     1141 tccatgacaa cggcgacggc gttcacgaag gtctgccaca gcttaaagat caccttcatg
     1201 ctcattcgtc aactgatcca tcagccaagt ttgaagatgt tttgacactt ctggacagta
     1261 tagggcagat cccgaaacca gtggtcgtga ccctgtttcg tgcttaggct cgacaaacaa
     1321 tccacgatac ccaagccagc cgaacaacca ggtgtcatcc tcgataccaa ttggaacatt
     1381 cgcacaatac agcagcgacg gttgaacaac ataggatgcc gcgacaagat cgaacggata
     1441 gaacgcgtcg atgccgatat cttcctgcca gtaggagagc caggggcggg catgatgggc
     1501 gacccatgca gcaggcccac ctcgtgcttc catcttgtcg aggcatgaag catccagaag
     1561 gatatccctc gccgcctcgt agggaataag tgtcaggggc aaacccatcg cgagaacgac
     1621 cgcggcggct tgctcgtctt ccgtcaagtt gaagtctctg aaaatcggac catgtcccaa
     1681 gaacgagtgt gccgtgcctc cttcgaccgg atggaacaca tgtccttttc gacggcccat
     1741 gacggcgacg agtcgcgtca cgttggcctg cagttgggga cggtccttta atgcgagcgc
     1801 gacgtttgtc agcgggccca gggcgacaat ggcgaggggt tcttcctgaa gagctttgcg
     1861 caacgcttca tgcgcctctc gtggccgaat gctttcgatc atttcttctt gcttgattgg
     1921 gtcggacgcc ctccggtata ccggaatcgc atgcgatctg ccggtttcga ggaaagcgat
     1981 cagatcgcgc gtcgtgcgat ccgttccatc gagaggggca ttcccaaaaa ttgtggacac
     2041 gcctacaagg tggatgcggt cttgctgcgc gagcagcaga atcgccaaac agtcgtccgg
     2101 atcggtccgt cgcccttttc cacaagcggc atcggtatcg atccagaccc tgaccggtga
     2161 ggtgccaaac ggctcggcgg gtcgcacttg aatcggctca gtaggaagtt ccccggtcct
     2221 ccacacttcg aacgggacca tcagcgaaag gactagggtt acgcctgcga ggagtatgag
     2281 aatgccgact gccgggattc caatgcgaac gagccgcaag atgctgtgca tgcccgatca
     2341 cctctgcttc tcgattctca tgaagtccca ctatcgatgc cttcttctca gcatgtctca
     2401 aacgggattc tcaggccgat tcgcgcgaat acgtcgcgca acactcgtga cggctgagaa
     2461 ggacagtcgc gcgaccgcca ggcgacgaaa ccgtccggac gtaccagcac tgcaccgctt
     2521 tctgtcacac cataggcaac gggccagcga ttctcgaggt caaccagatc gccgccggaa
     2581 ccgatacgat agcattgcaa gttatatggc ctgcgatcgg cgatcgccaa tgctgcttca
     2641 caccatgccg tgccggcggc tccggtcaac aacacaaaat ccttgttgaa gaggtcaagc
     2701 gtggacatcc gccggccata gtgctcaata accagatgtg gcgcgcgaga cccaggctgc
     2761 cctgacagtt cgcgtgcctt cgcaaacgca cgaccggcgg tctctccttc cgtcatcacc
     2821 gcaccggtgt gatagagatg accaaagacg acgtcatagt cgtcaacgag gggcacaggt
     2881 ggcggcaaac gcttattcgg atctttaaac caggcttgta gcctcgtcaa tgcttgttcg
     2941 agtgaagttt cagccaccgg ccggcgttca cggtcgtaac tgtcgagtag cgccgggtcg
     3001 gctctgccct gtagcacggc agccagtttc catgccaaat tctgggcgtc atgaatcccg
     3061 gtattgccgc cgaacgcgcc ggtgggcggc atcagatgag cggagtcacc cactaagaat
     3121 acgcggcctt cacggaaacg accggcgatc agggccgcga cttcccatga tcgcgcatcg
     3181 accaggtctg ctttgacatc cgctcgcccg gccccccggc ggacaagtac tcgacaacga
     3241 tcgtcgtcaa aatcttcggg gctctcatcg cgctcgggaa agtattgcaa cgccagcaac
     3301 cagcgtccgt tctcacgagg ggtaaaggtg ccattgatct cggtcaggaa gcaggaggtg
     3361 aagcgcttcc cctgcacagc cggcggcagg tcggtgtcaa agatgatatt catccagtgc
     3421 tgaagcactc cgggaccgtt acgtgcaatc cccacggctt cgcgagtggt tccgttcgct
     3481 ccgtcggcgc cgatcaggta cgcggcctga acctcctgct cctcgccggt ttcacggttg
     3541 cggatgagag cccgcacttc tccgtcctgc acgaacgaca ccagttcggt attgaagcgc
     3601 acatccgcac cgagacgctc cgcatgagcg cgcaagatcg gttcaagaac atgttgatca
     3661 caggtcgccg gcggcgtcgg gctcaggccg ttgatatccg gccaagcatg ggggcgcatc
     3721 cattcaatgg ccgggtcagc cagattcttt ccacgagcga cgccccctgc ctgttgatcc
     3781 cccgttcgtt ttgcgcgaat ttcttcttcg atgccgagcg aacggaaaat ctccatgctc
     3841 cgcggagaga tgccggcgaa tttgtattgg atggaggtgt tgggatgacg ttcgaccacc
     3901 aagcactcga ctccatggtg ggccagcagc aaggcggtcg tgaggccatt gagaccgccg
     3961 ccgacgatca gcacctctct attgatgggc accgtaccct cgatgcgttt gatgcagatc
     4021 aaggttctgc cctcatgacg gtaaacggat cgccgatgag ccaacgccat gtaccgtcaa
     4081 gctgacggcg ggcgacttca atcgcgtaca ctttcttagg ctgaggtccg gacaccgtcc
     4141 actgcgtgtg catcagagcg atgtcaccgg tctgcacggt ctgcttgatg gtgaaatcga
     4201 aacgagtctt catagccgca aaggtggaca actcttgttt caatccgtcg ccgtttttta
     4261 cttcccccgc ctgattgaga aagaccgctt gcggatcgta aaggttaagc agagcgtcaa
     4321 gatcgccttc agccatgtgc tctttgaaaa ggcggcagat ttcttcagga ctgtttgccg
     4381 gcatttctta ttcctccgct tgagaacatc aggcactgcg ttcatcatcg cttcttcgag
     4441 aggacgacat gcgtgacact cttgggcctg cgccgtaccc gtccagtgat atcgagaagc
     4501 tgtggacacg aagtttgctc atatgtcggg cctatggaaa gagcgatgtc gccatggcga
     4561 tcgctatcag ggccagcaca tgcaggcatg ctctgaccgc gtgcgagcgt tcccaagcgt
     4621 ctcgcagttc cgtccagtcg ccggtttgtg tgttcgcaaa tgtcgagaag aacgccaacc
     4681 cagcccccga tgtggccacg tccttgaccc aaaagttgtt gatcgggtgc gtcatcagcc
     4741 aatagaccgt gtgcgccgcc aacatcaaga gcagggctcc agccgtccac cacacggcgc
     4801 ttccccggcc attccagcgc atgcgccagc gaagtggcca tggtgacggc gacgagcagt
     4861 aaggagagaa ccgttaaaaa ccgcatggtc gttctctatc aatcactgtg gagttagaag
     4921 agaatgcgtt cgccgatcgg gatcaccggt gatgcgaggt tgcaagcact gcttcctcgg
     4981 tgtcgacgac cgacgttggg ctctcacgcg gaactgagca cttgttcctt cgtcggaacc
     5041 ccaaaagtca tgctttcgct cttgcagcct tcacgccgct tccccgccgc gctgcctcga
     5101 tggcggcgac gtcaatcttc tgcatggtca tcatcgcctc gaaggctcgt cttgcctcct
     5161 cgccgcctgc cgcgtgcgcc tgcatcaaga cgcgcggcgt gatctgccag gagacgcccc
     5221 acttgtcctt gcaccagccg cattcactct cctggccgcc attaccgaca atggcgttcc
     5281 agtagcggtc ggtctccgcc tgatcgtccg tcgcgatctg gaacgagaag gcctcgttgt
     5341 gcttgaaggc gggaccgccg ttgaggccga tgcaggagat accggcgacg gtgaattcca
     5401 ccatcagcac gtcgcctttc ttgcctgacg ggaagtcgct cggagcgcgg tagaccgcgc
     5461 cgacggaaga atcgggaaag gtcttggcgt aaaaccgcgc cgcctcctcg gcatcgcggt
     5521 cgtaccagag acaaattgtg ttcttcgcag gttgtttcgt catgtcattc ctccatcatg
     5581 accggcagtt gaagccatgg tcgatttcgc gcgaatacaa cccgaagcgc gccggttttg
     5641 aactcagcct aatcatcatg ttttggtttg cgtcagccgc ctatttcttc ggcttttccc
     5701 aaagagccat gacattgcct tccgaatccg cgaagtcggc catgaatccc cactcaccga
     5761 tgggatgttt cggcgtcacc actttcccgc ctgccttttc gacggccttg agcgtgttat
     5821 cgatcgaatc ggtttcgacg ccgaccgacg ggtggtcctc tttggatttg cgtttataga
     5881 actccccgtt gatgtcgcca ggctcggttg gatggtgctc tttatccgaa gccgtggtga
     5941 acgccatggc gccttcgttg ccctccggct caaccttcca gtcgaacacc gtttcataaa
     6001 atgtcttggc tttgttgaca tccgaagccg ggatctcaaa aaaacagact ttgtttttca
     6061 tgctcgatct cctttcttgg tctcaggttt cagcgagggt tttaattttg tcagcacttt
     6121 tttgtcttca tcacatgccg gccggttctt ctggtgaagt gattcacttc tca
//
LOCUS       DATMTI010000505         1730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_528941, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000505 DATMTI010000000
VERSION     DATMTI010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528941"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VN039_08150"
     CDS             complement(<1..357)
                     /locus_tag="VN039_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45974.1"
                     /translation="MDIMATPRQRQVVAKSPAISRFAKNPTFKQQVHDTDVQPVVHPL
                     VQKAHAAQTVRPTAPQQHHNAQTKKQSVVTKALAEAKPVKAKKTSLKKRFPRLISVAS
                     ASLAVLLIAGYLTYLNM"
     gene            676..>1730
                     /locus_tag="VN039_08155"
     CDS             676..>1730
                     /locus_tag="VN039_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HWV45975.1"
                     /translation="MYRSPSTFRHILTRVIVYLLMTVAVGCLVIGSLLYILGYRFDNK
                     NHRIERSGLVQYITKPSGAEIAVDSIKLSQRTPTNTTVSSGRHTFTFTRGGYQTWEKT
                     LTIKPGTLTWLNYARLIPNDIAIESVSALPQAAQALATPGRQYMAVLTRSDRPAIQVY
                     DLTGDTVRTSTMTASADEYDTTKTSAKHVFQMVEWDDNDRYLLVKHTHGKVVEWLVFD
                     RQTGKLISNVTKTMDVVIRSAHIIDQGGPVVVALIDDDIRKIDLNSETVSRPLVSRVD
                     DFSVFDDDAISYVSAPDETTKQRAVGLLANGGKPVVVYTSKKGETQPIHVVSGHYFDK
                     DYIAISDGKQVILQSGT"
BASE COUNT          372 a          428 c          466 g          464 t
ORIGIN      
        1 catattgaga tacgtcagat aaccggcgat cagcaggact gcaagactgg ccgaggcgac
       61 gctgataagg cgcggaaagc gcttcttcaa gctggttttc ttcgctttga ccggcttagc
      121 ttctgcgagg gccttggtga cgacgctctg cttttttgtc tgtgcgttat gatgttgttg
      181 tggtgcagta ggccgtacgg tttgcgcagc gtgcgctttt tgcaccagtg gatgtacgac
      241 gggctggacg tcggtatcgt ggacctgctg cttgaaggtt gggtttttag caaaacggct
      301 gatagcggga ctctttgcga ctacctgacg ctgacgcggt gtggccatga tgtccatact
      361 acgtcgtccc gtagtttttg gtgcagctgt ctttttctta acagtgcgac gattgagcgt
      421 cgtcgatttc tgctgagtag catggaccgt ctgagccgga accgtcgcgt gatcgcggac
      481 atgcgctttc gtcttctcgg cggcttcaag gacgtcgaca gggaggcccg tgtgtgggtc
      541 gtaccgtctt ccgtttatgc tgactatatg tgtgttgccc atcgttactg aatgttttcc
      601 cctactgtga ttactttata tagtagtgac tgcagcatct ttttgcaagt tattttgtta
      661 taataagcgg tagttatgta tcgttctcca tcgacgttcc gtcatattct cacccgtgtc
      721 atcgtgtatc ttctgatgac ggtagctgtt ggctgcctgg tgatcggctc gctgctctat
      781 attctgggtt atcggtttga caataagaac caccgcattg agcgcagcgg actggtgcag
      841 tatatcacta agccgtccgg cgccgagatt gccgttgaca gcataaaact cagccagcgg
      901 acgccgacga acacaaccgt cagttcgggc cgacatacct ttaccttcac gcgtggcggt
      961 taccaaacct gggaaaagac gctgacgatc aaaccgggga cattgacttg gcttaactat
     1021 gccaggctga tcccaaatga catagcgatc gaatccgttt ctgccctgcc ccaagccgct
     1081 caagcgcttg caactcccgg acgtcagtac atggcggtgc tgacacgctc cgatcggcca
     1141 gccatacaag tttatgacct aacaggagat accgttcgca ccagtaccat gactgcatcg
     1201 gcggatgaat atgacacaac taagacgagc gccaagcatg tctttcagat ggttgaatgg
     1261 gatgataacg accgttatct tttggtcaaa catacgcacg gtaaagtcgt cgaatggctc
     1321 gtttttgatc gccagaccgg caagctgatc agcaacgtga caaagacgat ggatgtcgtc
     1381 atacgttcgg cgcatattat cgaccagggc gggccggttg tcgtcgcttt gattgatgat
     1441 gacattcgta aaattgattt gaatagcgag accgtttcgc gtccgctcgt atcacgcgtc
     1501 gatgatttca gtgtctttga tgatgatgcg atcagctatg ttagcgcacc ggacgaaacg
     1561 accaagcaac gtgccgtcgg tcttctcgcc aatggcggta agcctgtcgt tgtctacacc
     1621 agcaaaaagg gtgagacgca gcctatccat gtcgtcagtg gccattattt cgacaaagat
     1681 tatattgcca tatccgatgg caaacaagtt atcttgcagt cgggtacctt
//
LOCUS       DATMTI010000506         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_401572, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000506 DATMTI010000000
VERSION     DATMTI010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401572"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VN039_08160"
     CDS             complement(<1..503)
                     /locus_tag="VN039_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWV45976.1"
                     /translation="MKRNLIDNSLWLLIGTAAFFSAVAPAQAQEKKPNILFIMGDDIG
                     MWNIGAYHRGMMAGRTPNLDKLAKEGMLFTDYYAEASCTAGRANFIMGELPIRTGMTT
                     VGQAGAKIGIPAEACTIATALKAQGYATGQFGKNHLGDLNEFLPTVHGFDEFFGYLYH
                     LDAMEDPA"
     gene            complement(759..>2132)
                     /locus_tag="VN039_08165"
     CDS             complement(759..>2132)
                     /locus_tag="VN039_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWV45977.1"
                     /translation="RPGTPCIAQFLYNLGYTTGQFGKNHVGDNPDSLPTAHGFQEFWG
                     YLYHLDAMQQVSFPDINKSPTEQAIVPPCKMVPIPGVPDVPGAIDPKDGTCMAAPRPV
                     LWMKSSDGTAKNQQGKDEGPLTLERSKTVDEEISSHVVDWLDRNDPKKTNKPFFCYYN
                     PARMHITTMFSPKYEAMLGVKGGKDWGINEVGMKQMDDNIGLVLKKLEDMGQLDNTIV
                     VFTTDNGAETQSFPDGGITPFKGTKLTTWEGGMRAPCVIRWPGHVKPGTVYKEMFAAL
                     DWVPTLVEIAGGPKGDELNKQIMSGKYKGIVKTKLDGFSQVDYLTGKSEKSARDTFFY
                     YMGHIPSAVRYKNWKFYYTMPAESASGGFLPPIPYKWTMIQNIMRDPFEQAVGAEQKT
                     LLSVGGAFAAASTAYIYDWNILPIGQLLWEKELMSYKEFPPLQEAASYNLDQILQSIK
                     EHSNPSQ"
BASE COUNT          414 a          585 c          599 g          534 t
ORIGIN      
        1 gccgggtctt ccatcgcgtc gaggtggtag agataaccga agaactcatc gaacccatgg
       61 accgtaggca ggaattcgtt gagatcgccg aggtggtttt tgccgaactg gcccgtggca
      121 taaccttgag ctttcagggc agtagcaatg gtgcaagcct ccgccgggat ccctattttg
      181 gcacctgcct ggccgaccgt cgtcatgcca gtgcgaatag gcagttcgcc catgataaag
      241 ttcgctcgac ccgccgtgca actggcctcg gcgtaatagt cggtgaacag catgccttcc
      301 ttggccagct tgtcgagatt cggtgtccgc ccggccatca tgccacgatg gtaggcgcca
      361 atattccaca tcccaatgtc atctcccatg atgaaaagga tgtttggttt tttttcttgg
      421 gcctgggctg gagcgacggc gctgaaaaac gcagcggtcc ctataagcag ccacaaactg
      481 ttatcaatta agtttcgttt cattgtctgt tcttctgttg ctgttttgat ttgatttcgt
      541 taagcgactc agaaaatttg ggctgtcgtt gcagctgtaa aatcgccctc tccgctaacc
      601 ccggcagtca ttaacgacgc caacttcatt accgcgacca atgatagggt tcttatgatt
      661 cttctccaat cgtatgtata tgttgatttg gtgagaggaa gcgggcggcc cgttttcggg
      721 ccgcccgacc tcgttatcaa tcctggaacg gcgatcgcct attggctcgg gttgctgtgc
      781 tccttaatcg actgcagaat ttgatcgagg ttgtagctcg cggcctcttg cagcggcggg
      841 aactccttgt atgacatcaa ttccttctcc cacagcagtt ggccgatagg cagaatgttc
      901 cagtcataga tgtacgccgt actcgccgcg gcgaacgcac cgccgacgga tagcagggtt
      961 ttctgttccg cgcccacggc ctgctcaaac ggatcacgca tgatgttttg gatcatagtc
     1021 catttgtatg ggatgggtgg caagaaacca ccgctggccg actcggcggg catagtgtag
     1081 tagaacttcc agttcttata ccgcactgcc gaaggtatgt gccccatgta gtagaagaac
     1141 gtgtcgcggg ccgacttctc agacttgccg gtgagatagt cgacctggct gaaaccgtcg
     1201 agcttggtct tgacgatgcc cttgtatttg ccggacatga tctgcttgtt caactcatca
     1261 cccttcgggc cgccggcaat ttctacgagt gtcggaaccc agtcgagcgc cgcaaacatc
     1321 tccttgtaca ccgtgccggg tttgacgtgg cccggccagc ggatgacgca gggagcgcgc
     1381 atgccgcctt cccaggtggt cagcttcgta cccttgaacg gcgtaatacc tccgtcagga
     1441 aaggattggg tctcagcgcc gttgtcggtg gtaaacacga cgatcgtgtt atccagttgg
     1501 cccatgtcct cgagcttctt aagcaccagt ccgatgttat cgtccatctg tttcatgccg
     1561 acttcgttga tgccccagtc cttaccaccc ttcacaccaa gcatggcttc gtacttcggc
     1621 gagaacatcg tcgtgatgtg catgcgcgcc ggattgtagt agcagaagaa cggcttgttg
     1681 gtcttcttgg gatcattacg atcgagccaa tcaacgacgt gggaagagat ttcctcatcc
     1741 accgtcttcg agcgctccag agtcagcgga ccttcgtcct tgccttgctg gtttttggcc
     1801 gtgccgtcgg aggatttcat ccagaggacc ggacgcggag cggccataca tgtgccgtct
     1861 ttcgggtcaa tggcacccgg gacgtccggc actccgggga tcggcaccat cttgcacggt
     1921 ggtacgatag cctgctcggt cgggctcttg ttgatgtccg ggaagcttac ctgctgcatt
     1981 gcgtccaagt ggtagagata gccccagaac tcttggaagc cgtgcgcggt cgggagcgag
     2041 tcgggattgt cgccgacatg gttcttgcca aactggcctg tggtgtagcc gaggttatac
     2101 aggaactgtg cgatgcaagg tgtacctggt cg
//
LOCUS       DATMTI010000507         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_103717, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000507 DATMTI010000000
VERSION     DATMTI010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103717"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VN039_08170"
     CDS             complement(<1..235)
                     /locus_tag="VN039_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV45978.1"
                     /translation="MLKLVGVTKTLGGQPVLQGIDLIVPEGKLTTIIGRSGEGKSVLL
                     KHMIGLLQPDSGQVWVDGIEISRLRGHALNEVRK"
     gene            complement(238..1005)
                     /locus_tag="VN039_08175"
     CDS             complement(238..1005)
                     /locus_tag="VN039_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HWV45979.1"
                     /translation="MTLLELIGRWALIYVAEMGRMLIFMVTSFAWLARPPLRGMQIVK
                     QLHFIGYKSTFVVVLTAAFTGMVLALQGYYTLRKFGSEGLLGSAVALSMIRELGPVLA
                     ALMVTARAGSAITAEIGIMRITEQIDALDTMAVNPFQYLIAPKLVAGLIGVPLLVAIF
                     DVVGIYGGHLVGVDLLGVNAGSYWNSIEAAVEWKDVYGGILKSISFGLIVSWVCCYKG
                     FYTKMSAEGLGTATTQAVVLSSVLILIWDYFLTSLLL"
     gene            complement(1008..1793)
                     /locus_tag="VN039_08180"
     CDS             complement(1008..1793)
                     /locus_tag="VN039_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45980.1"
                     /translation="MPPKRIAIFAATPWEMGAVRSAFPHGVERRIGRLPVFVRIVGNR
                     EYWLAQTGVGLEKAGRNAAHLLDNQSFSLIVSTGFACALNAAHIGTLLVGREVVHQGA
                     RSDEPSEVLDVPGDERDLVMTWVTSLVPPEHIGRFVSTDRVIGSAREKRRFALSTQAI
                     GLDMESSALAAQAQRARVPFVIIRSVSDLLDEDLPLDFNLFLRPTGWLKGIGAVLAAP
                     SCLLGLGRLRRQSLVAAEALTAFFRNYAEAMATGRRKKEFSPT"
     gene            complement(1832..4015)
                     /gene="shc"
                     /locus_tag="VN039_08185"
     CDS             complement(1832..4015)
                     /gene="shc"
                     /locus_tag="VN039_08185"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWV45981.1"
                     /translation="MNIIKALFNRLSDSLLATVQGRLDPATEFTPIAPLRLVSDKPIV
                     LPSLDSSAARRPIGHAVSQPDAVDDAIRRSQSWFFSRQHAEGYWVAELEADTTLTSEY
                     LMLRRFLDRVDPDREVKAVRYLKMMQLPDGGWPIYYGGPAEISASVKAYFALKLSGVS
                     ADEPCMVRAKERILAMGGVLQANVFTKITLALFDQYDWEGVPHMPVELMLLPKKFYFS
                     TYAISYWSRAVLIPLLIVFAHRPVCRIPCEQGIDELYPIPRAEVRYWKYPPFNKDQAW
                     FTPHNFFVALDAMLKLYDRMPMRMLREKALHKAACWMVDHVKGAGGLGAIYPAMANSI
                     MALECLGYDADDPLVVKALREIEELEVYDSTTIDGEIVPTLHLQPCFSPIWDTALLMN
                     ALVEAGVPQDHPMLQKAGRYLMSRQTKTVGDWIISSPNAEPGGWYFQFENELYPDVDD
                     SAVVIMALSKLKIPGREGELNESIRRGMRWVLAMQGSDGGWGAYDKDNNRIVFNYIPF
                     ADHKALLDPSTSDLAGRCLEMLGALGYDKTHPAAGPALAFLKKEQEEDGSWYGRWGIN
                     YIYGTWSVLAGLRAIGEDLSAPSIRRAVAWLESKQNSDGGWGESGLSYKDDPEHHGRG
                     ESTPSQTAWALMALMSAGAIDSFSVARGVQFLLRRQMKDGSWEEIAHTGTGFPRVFYL
                     RYHWYCQYFPLWALAMYRNLRSRGKMRADELRHHIQGTDSYRSEH"
     gene            4567..4779
                     /locus_tag="VN039_08190"
     CDS             4567..4779
                     /locus_tag="VN039_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45982.1"
                     /translation="MYHIGMFTFEVVRAPLTVASAQTYAETGLASWYGEETRRQPGGY
                     MTANGELFNPNALTAAHKHLSLPTHV"
BASE COUNT         1181 a         1410 c         1262 g          967 t
ORIGIN      
        1 gtttgcgcac ttcgttgagt gcatggccgc gaagccgaga aatctcgatt ccatcgaccc
       61 atacttgtcc ggaatcaggt tggaggagcc cgatcatatg cttcagcagc acgctcttgc
      121 cttcaccgct tcgaccgatg attgtcgtga gtttcccttc ggggacgatg agatcaatgc
      181 cctgcaagac cggctgacct cccaacgtct tcgtcacacc cacaagtttc agcatgatta
      241 caacaacaat gacgtcaaaa aatagtccca gataagaatc aagaccgatg acaatacgac
      301 cgcctgtgtc gtggcggtgc cgagcccttc ggcactcatc ttggtataaa agcccttata
      361 gcaacagacc cagctcacaa tcagaccaaa actaatggat ttgagaatgc cgccatagac
      421 gtccttccac tcgaccgcag cctcgatgga attccaatac gaaccggcgt tgacgccgag
      481 caggtccacg ccgacaaggt gcccgccgta gattcccacc acgtcgaaga tcgcgaccag
      541 caggggaacg ccgatcaaac cagccacgag cttcggcgca atcaggtatt ggaacggatt
      601 gacggccatg gtatcgagcg cgtcgatctg ttccgtgatc cgcatgatgc cgatctccgc
      661 tgttatcgcg gaaccggcac gcgccgtcac catgagcgcg gccaagacgg ggcccaactc
      721 acgaatcatg ctgagcgcca ctgcggagcc caacagccct tcagacccga actttcgcag
      781 cgtatagtag ccctgtagcg ccagcaccat cccggtgaac gccgccgtca gcacgacaac
      841 aaacgtcgac ttgtagccga tgaagtggag ttgcttgacg atttgcatgc cccgcagcgg
      901 cggacgcgcc aaccaggcga aggacgtgac catgaagatc agcatccgtc ccatttccgc
      961 cacatagata agcgcccatc ggcctatgag ttcgagtaac gtcatggtca agtcggcgag
     1021 aattccttct tccggcgtcc ggtcgccatc gcttccgcat agttccggaa aaaagccgtc
     1081 aaggcctccg ctgcgaccag actctgtcga cgaagccggc caagacccaa gagacatgaa
     1141 ggagcggcca aaacggcacc gatacctttg agccatccag tgggcctgag aaacagattg
     1201 aagtcaagcg gaagatcttc gtccaaaaga tctgaaacag atcggatgat cacaaaaggg
     1261 acccgtgccc gttgcgcttg tgcagccaaa gctgaacttt ccatatcgag gccgatggcc
     1321 tgagtgctca gcgcaaacct tcgcttttcc cgggcactgc cgatgactcg atcggtcgag
     1381 acaaatcgcc cgatgtgctc agggggcacc agactcgtga cccacgtcat gacgagatcc
     1441 cgctcatcac ccggtacatc gagaacctcc gacggctcgt cgctgcgcgc tccttggtgc
     1501 accacttcac ggcctaccaa gagtgtcccg atatgcgccg cgttgagcgc acaggcaaac
     1561 cccgtcgaca cgatgaggct gaatgactgg ttgtcgagca ggtgggcggc gtttctcccc
     1621 gctttttcaa gaccgacgcc tgtttgagca agccaatatt ccctgttgcc tacgatacgc
     1681 acgaacaccg ggagacgacc gattcgacgt tcaacgccgt gagggaacgc cgatcgcact
     1741 gcacccatct cccatggagt ggcggcaaaa atagcgattc gtttaggcgg cacagtacca
     1801 gatggacagg gggaaagaga accgacgggg gtcaatgctc ggaccgatac gagtcagttc
     1861 cttgaatgtg atgacgaagt tcgtcggccc gcatcttccc acgggaacga aggttacggt
     1921 acatcgccag agcccacagc gggaagtatt gacaatacca atgataccga agataaaaca
     1981 cgcgcgggaa tcctgtaccg gtatgagcaa tttcttccca cgatccatcc ttcatttgac
     2041 ggcgaaggag gaattgcact cctcgcgcga cactgaagga atcaatcgct cccgcagaca
     2101 tcagggccat caaggcccat gcggtctgtg agggggtgct ctcccctcta ccgtgatgct
     2161 caggatcgtc cttataggag agaccggact cgccccaccc accatcagaa ttctgtttcg
     2221 attccagcca agcaaccgcc cgtcgaatag aaggtgccga gagatcttcg ccgatggccc
     2281 tgagcccggc taaaaccgac caagtcccat agatgtaatt gatgccccat cgtccgtacc
     2341 aactgccgtc ttcctcctgc tctttcttca gaaaagccaa ggccggtccg gcggctggat
     2401 gggtcttgtc atatcctaat gcgccgagca tctccaggca tcgcccggcc agatcggagg
     2461 tactggggtc caggagagct ttatgatcgg caaaggggat atagttgaag acgattcgat
     2521 tgttgtcctt gtcataggcg ccccagccgc cgtcggatcc ctgcatcgcc aagacccatc
     2581 gcattccgcg acgaatggac tcgttcagct cgccctcccg acccggaatc ttcaatttgg
     2641 agagcgccat gatgaccacg gcggaatcgt cgacatccgg gtacaattca ttctcaaact
     2701 ggaagtacca cccccccggt tccgcgttcg gcgaggagat aatccagtca cccacggtct
     2761 tggtttgcct ggacatgaga taccggcccg ccttttggag cataggatga tcctgcggca
     2821 ctcccgcttc aaccagagcg ttcatgagca acgcggtgtc ccaaatagga gaaaaacagg
     2881 gctggagatg gagagtggga acgatctcac cgtcgatcgt cgtggaatcg tagacttcca
     2941 actcttcgat ctcgcgaagg gccttgacga ccagcgggtc atcggcgtca taccctaagc
     3001 attcaagcgc catgatggag ttggccatcg ccggatagat ggcaccgagt cccccggccc
     3061 ctttgacatg atcgaccatc caacaggctg ctttatgcag cgccttctcc cgcaacatcc
     3121 gcatgggcat ccggtcatac agtttcaaca tcgcatccag tgccacgaag aagttatgcg
     3181 gagtgaacca tgcctgatcc ttattgaacg gagggtattt ccaatagcga acctccgcgc
     3241 gcgggatcgg gtacaactcg tcgatacctt gttcgcatgg aatccggcag accggacggt
     3301 gcgcgaatac gatgagcaag ggaatgagca cggcccgtga ccaatacgag atcgcataag
     3361 tactgaagta gaacttcttc ggcaacaaca tcagctcgac gggcatgtgg ggaacacctt
     3421 cccaatcgta ctgatcgaac aaggccagcg tgattttcgt aaacacgttg gcctgcagga
     3481 caccgcccat cgccaagatc cgttctttgg ctcgaaccat acacggttcg tccgccgaca
     3541 caccgcttaa tttgagcgca aaatatgcct tgaccgatgc gctgatctcc gccggcccac
     3601 cgtaataaat cggccatccg ccgtcgggga gttgcatcat tttgaggtat cggacggcct
     3661 tcacctctcg atcaggatcc acacgatcga gaaaacggcg cagcatcaag tattcggagg
     3721 tcagcgtggt gtctgcttcc agctcagcga cccagtaccc ttcggcatgc tgacggctga
     3781 agaaccacga ttgactccgc ctgatcgcgt catccacggc atcgggttgg ctgacggcat
     3841 ggccgatggg ccggcgagca gcggaagaat ccaatgaagg caggacaatc ggtttgtccg
     3901 agaccaaccg aagcggagca ataggggtaa actccgtagc tgggtcgagt cgcccctgaa
     3961 cggtcgcgag caggctgtca gagaggcgat tgaataacgc ctttatgatg ttcatgaatg
     4021 gttaggactc aaaagagaca actgcggcag gcgaaagacc gacatcttac aatggtctcc
     4081 cttcgtaaat ctttccaaca ctccttacta atacacggaa ggcttataac aaacgattga
     4141 aggcgagtca agcgaaggag ctgagaagtg cctgaagtat ctatattttt tatcggtgta
     4201 ggcaatcgag aacaccacgt taacagcatt cgtagctgca cgatgcacca tcaatctccg
     4261 ctgtcaatca tgggtgtgat agaaccaagg tcatcaacca cctgcatccg tactttcctg
     4321 gtatttcaat gcctcagttc ggttcttcat ccgaaactca gataagaggc gttgacctaa
     4381 ggatgaacag gctcacgctt tcattgttgc tgcttgtcat caccatgacg ctcacctcct
     4441 gttcggtgat caggacaacc ttctcggtga tcaagacgac gtacaagacc atccaaaggg
     4501 acggtcaaag gcacggtatg ggttgttcga ggaacctatc aacttactaa ggagaccacc
     4561 agactcgtgt atcacatagg tatgtttacc tttgaggtcg tccgcgctcc attgacggtc
     4621 gccagcgcac agacctacgc agaaacgggc ttggcgtctt ggtatgggga agaaaccagg
     4681 aggcagccag gaggctatat gacggccaac ggcgaactat tcaatcccaa cgcattgacg
     4741 gcagcccata agcatctatc gcttcccact cacgtatagg tgacaaacct agaaaacgga
     4801 aggtcgatta ttgtccgagt
//
LOCUS       DATMTI010000508         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_826191, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000508 DATMTI010000000
VERSION     DATMTI010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826191"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VN039_08195"
     CDS             <1..904
                     /locus_tag="VN039_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWV45983.1"
                     /translation="MSLDYVKFSNGFEKFMPKEYRDMVEHGPFGKKVTVSQMGSFKEV
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTNRGAVLKMAPLGKKFEKMDMLQMAKVAGCAYVATVVPNNPRRVESVI
                     KKAVLIAREVGSTYIQAYTSCNIEYAIPTDKVMEDAKAVENDRYQFTEYVSEEAKQYL
                     TERYGYKEFLAKPAAPAAAIPGKA"
     gene            926..1642
                     /locus_tag="VN039_08200"
     CDS             926..1642
                     /locus_tag="VN039_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWV45984.1"
                     /translation="MAKRFNIRMAGVGGQGVVTGSHILSTAVINAGGESTIVPFYGSE
                     KRMAPVESYVRVSDEPIYEIGEITFPHIIIIFHPQVITHGKSYTMPFYFGLKEDGIAL
                     INNDGPMNLHRDQARELQERRAKLYYFPATKISLEVAGMDLATNMALMGCIGAITGLT
                     SMAGLDQAVKDRFLGKGFVVSGGTAALDSVVERKFKKKQELIEKNVAVMRAGWNYAVD
                     HGWASADVKRAEEPVAAATA"
     gene            1663..1986
                     /locus_tag="VN039_08205"
     CDS             1663..1986
                     /locus_tag="VN039_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HWV45985.1"
                     /translation="MYLVADISVEICAAKSCKLCTQYCPEANTIQYSDELGKDQGFKY
                     GSAYVAVDRCKGCAQCVWVCDNMAKNNAIKMIMIDQLPKAALTENITYREKSTTAVLA
                     SPVVG"
     gene            2008..2772
                     /locus_tag="VN039_08210"
     CDS             2008..2772
                     /locus_tag="VN039_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HWV45986.1"
                     /translation="MATTQDTRERIIVPGPAGFHPPSAAQLGVSLPDPGEGLFYGLLE
                     PNEEVVIEEMARKMLTSPNATLFPGPLLLWAWNDHAVEKAKATLEIAAQIPEVMIIPM
                     PDYRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCCT
                     MAICAEQGHEDAMLTIRDSDTAKLKRVAQIFKRVREEMGIKLPENGENVRFTGTQSKV
                     HDGKTHTNPIAFAPTPGGAGSAAIFGHSAEQMKREG"
     gene            2812..>4102
                     /locus_tag="VN039_08215"
     CDS             2812..>4102
                     /locus_tag="VN039_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWV45987.1"
                     /translation="MSETEVKTNPQGDPITSVAVPKSDGKKDPHGEAKRQQVVTPEYM
                     FLEAPRTKEFITGSEAAKEAVRRSNVDLAIAYPITPQSETMQLVGVLYGEGYVKEYYR
                     GEEEVGVMAAIAGGSRSGVRCYTATAGPGTLRGLEGIASWPGHRLPVVAMFTCRVVNA
                     PLAIQPDNIEVSYLLNCGMIVFHAENQQDMFDFTMAGFTISEKNDVTLPVGVCCDGFF
                     VTHARGYVRMQDRGIKLPPREAWRGAVPVLDAENPPARLSRDAPVQKSNFMAYNIHAV
                     WQQEVWAAVERSRKYINKYMGGLLTAENVEGAEAIIIASGSAAAQSREAVRLCKDKGL
                     NVGLIKVRSLRPFPTKELRELCKNTKLIVVPEFNYVGWLAKEVATAIYGYSKAKIIGG
                     PRVYGGQSMPVELILDEVESGLTGKKSTNVAMSQVMGG"
BASE COUNT          980 a         1031 c         1245 g          846 t
ORIGIN      
        1 gatgagtctt gattatgtca agttttccaa tggatttgaa aagtttatgc caaaggaata
       61 tcgggacatg gtggaacatg ggcctttcgg aaagaaggtc actgtttcac aaatgggaag
      121 ttttaaggaa gtgttggaag aacatcccat gtgcgccggc tgtgctatga cgctcttcat
      181 tcggctggcc atgattgcgt ttcctaatcc cgaagacacg attactgtcg ggacggctgg
      241 ttgcggtcgt ctggccatct ctcaggcggc gattcccttt gtctacggca actacggtga
      301 ccaaaacgga gtggcgagcg gattgtctcg tggcctccgt cttcgattcg gcgacaagcc
      361 gaaggacgtg gttgtgatgg cgggtgacgg cggtacggcg gacatcggct tccagcaggt
      421 ccttcactcc tggttccgca aagagcggtt cacgacgatc atgttggaca atgaagtcta
      481 cggaaatacc ggcggccagg agagcggcat gaccaaccgc ggcgccgtgc tgaagatggc
      541 accgctcggc aagaagtttg agaagatgga tatgttgcag atggcgaagg tcgctggttg
      601 cgcctatgtc gccacggtgg tgccgaacaa tccgcgccgt gtggaaagcg tgatcaagaa
      661 agcagtgttg attgcccgcg aagtcggatc gacctacatt caggcttaca cgtcctgcaa
      721 catcgaatac gccattccaa ccgacaaggt catggaagat gcgaaggcgg tcgagaatga
      781 tcggtatcag tttacggaat acgtcagcga agaagcgaaa caatacctca ctgagcgtta
      841 tggctataag gaattccttg ccaagccggc tgctccagcc gccgcgattc ccggcaaagc
      901 ttaggcgttc acgaggaggt ctatcatggc aaagcgattc aatattcgga tggcaggcgt
      961 cggaggtcag ggtgtcgtca ccggctcgca tattctcagt accgccgtca tcaatgccgg
     1021 cggcgaaagc accattgtgc cgttctatgg gtcggaaaaa cggatggcgc cggtcgaaag
     1081 ctatgtccgg gtatcggatg agccgattta cgagatcggt gaaatcacgt ttccacatat
     1141 catcatcatc ttccatccgc aggtcatcac ccacggcaag tcctatacga tgccgttcta
     1201 tttcggcttg aaagaagatg gaattgcgtt gatcaacaat gatgggccga tgaatctcca
     1261 ccgagatcag gcgcgtgaac tccaagaacg tcgcgcgaaa ctctattact ttccagcgac
     1321 gaagatttca ttggaagttg cgggtatgga cctcgccacc aacatggcgc tgatgggttg
     1381 tatcggagcg attacaggtc tgacgagtat ggcgggattg gatcaggccg tgaaggatcg
     1441 gtttctagga aaaggctttg tcgtgtccgg tggtacggct gcgctcgaca gcgttgtcga
     1501 gcggaaattc aagaagaaac aggaactgat cgagaaaaac gtggccgtca tgcgggccgg
     1561 atggaactat gccgtcgacc acggttgggc ctcggctgat gtcaaacggg cggaggagcc
     1621 agtggcagca gccaccgcgt aataggtcac aaaggagtct tcatgtacct tgtagctgat
     1681 atcagcgtcg aaatctgtgc cgcgaaaagc tgtaagctct gcacgcaata ttgtccggag
     1741 gccaatacta ttcagtacag cgacgagttg ggcaaggacc aaggattcaa gtatggttcg
     1801 gcttacgtag cggtggatcg ctgtaagggc tgtgcgcaat gtgtgtgggt ctgcgacaac
     1861 atggcaaaga acaatgcgat caaaatgatc atgatcgatc agctgcccaa ggcggcgctg
     1921 accgagaaca tcacctaccg cgaaaagagc acgacggccg ttttggccag tccggtcgtc
     1981 ggataagacc tgaggaaagg ggaaggcgtg gcaacaacac aggatactag agagcgcatc
     2041 atcgtaccgg gccccgcggg cttccatcct ccgtcagccg cgcagttggg ggtgtcgctg
     2101 ccggatccag gggagggatt attttacgga ttgctcgagc cgaacgagga agtggtgatt
     2161 gaagagatgg ctcgtaaaat gctgacgagc ccgaacgcga ctctcttccc aggacctctg
     2221 ttgctctggg cctggaacga tcatgcagtg gaaaaggcga aggcaacgtt ggaaatcgcc
     2281 gcgcagatac cggaagtcat gatcattccg atgccggact atcgcccgaa gtatccaaag
     2341 atcgacccgg aagaagtcat caatcccaat caccccaatc tcacaatctg gggcaataaa
     2401 attgaagcct gtatcttcat cggcgtacat tgtcattatg cgaatctgac cctcaagatg
     2461 atccgggcgg gaacgaattg ctgtacgatg gcgatttgtg ccgagcaggg gcatgaagac
     2521 gccatgctca ccatccgtga ctccgatact gccaagttga aacgtgttgc ccagatcttc
     2581 aagcgggtgc gcgaagaaat gggcatcaaa ctgccggaga acggcgagaa cgtgcgcttt
     2641 accgggacgc aatccaaagt gcatgatgga aagacccaca ccaatccgat agcgtttgca
     2701 ccaaccccag gaggggcagg gagtgcggcg atcttcggtc attcagcaga acagatgaaa
     2761 cgagaggggt aggttgagcg cgcagctcac catctttata gaggtgcaac tatgagcgaa
     2821 accgaagtca aaaccaatcc gcaaggcgat ccgatcacca gtgtcgcggt gccaaagtcg
     2881 gatggaaaaa aagatcccca cggggaagcc aaaagacagc aggtcgtgac tcccgagtac
     2941 atgttcctcg aagcgccgcg gacgaaggaa ttcatcactg ggagcgaggc ggcgaaggaa
     3001 gcggtccgcc gctcgaacgt ggatttggcc atcgcctatc caatcacccc gcagagcgaa
     3061 accatgcagc tggtcggcgt actctatggc gaaggctacg tgaaagagta ttatcgtggt
     3121 gaagaagaag tcggtgtcat ggcggcaatt gccggtggat cgcgctcggg tgtccgttgc
     3181 tatacggcca cggcaggacc tggtacgcta cgcggtctgg agggtatcgc ttcatggccc
     3241 ggccaccgtc tccctgtcgt tgccatgttt acctgtcggg tggtcaatgc gccgctcgcc
     3301 attcagccgg acaacatcga agtgtcttac ctgctgaact gcgggatgat cgtatttcat
     3361 gccgaaaacc agcaggacat gttcgatttc acgatggccg gcttcacgat cagtgaaaag
     3421 aacgatgtga ctttgccggt cggagtttgt tgcgatgggt tcttcgtcac tcatgcccgt
     3481 ggctatgtgc ggatgcagga tcgcggcatc aagttgccgc cgcgcgaagc ctggcgtggt
     3541 gcggtcccgg tgcttgatgc agagaatcct ccagcgcgcc tgtctcgcga cgcgccggtt
     3601 cagaagtcga acttcatggc atacaacatt cacgccgtgt ggcagcaaga ggtttgggcg
     3661 gcggttgaac gctcccgcaa gtacatcaat aagtatatgg gtggcttgct gacggcggaa
     3721 aacgtcgaag gtgcggaggc tattatcatc gcttccggca gcgcggcggc ccaatcgcgc
     3781 gaggcggtcc gcctttgcaa ggacaagggc ctcaacgtcg gtttgatcaa ggttcgctcg
     3841 ctccgtccgt tcccaacgaa ggagctgcgc gagctgtgca agaatacaaa gttgatcgtc
     3901 gtgccggagt tcaattatgt cggctggttg gccaaggaag tagcgaccgc catctacggg
     3961 tactccaaag ccaagattat cggagggcca cgtgtgtacg gcggccaatc gatgccggtg
     4021 gagttgatcc tggatgaggt cgaatccggc ctgaccggca agaagtccac gaacgttgcg
     4081 atgtcgcagg tcatgggggg ag
//
LOCUS       DATMTI010000509         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_61273, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000509 DATMTI010000000
VERSION     DATMTI010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61273"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2089)
                     /locus_tag="VN039_08220"
     CDS             complement(<1..2089)
                     /locus_tag="VN039_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HWV45988.1"
                     /translation="MSHFLDRLLFFRENLDTFSGKHGVVRREDRTWENAYRDRWNHDK
                     VVRSTHGVNCTGSCSWKMYVKNGLITWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     VYSAQRIKYPLVRARLMALWREARKTLEPVEAWASIVEDTDKREQYTSIRGQGGFVRA
                     TWDEVTELIVAANVYTIQRYGPDRIAGFSPIPAMSMVSFAAGSRYLSLIGGVIPSFYD
                     WYADLPPSSPQVWGEQTDVCEAADWYNSSYIVVWGSNIPQTRTPDAHFYTAARYKGAK
                     TVAVAPDFAEYVKFADLWLAPRRGTDAALAMAVGHVILKEFHLTARSPYFLDYCRQYS
                     DMPMLVFLRPAGDGYAPDRFVRASDFDGQLGETNNPEWKTVAVDEATGHLYAPQGSIG
                     FRWREKGRWNLDLIDATTGHRITPALSVLPQAEEVVSVAFPYFSDDHPQILRRLIPAR
                     RLSTVHGDQLMVTAYDLLLASYGLDRGLGDDQAAKDYDQDIPYTPAWAEKHTGVHRRQ
                     IIRLAREFAENAEKTRGRSMVILGNGINQWYHTDMIYRAIINVLVMCGCVGRSGGGWA
                     HYVGQEKVRPLAGWTTVAFALDWVRPPRHMNGTSFFYAHTDQWRYESLRPAEILSPTA
                     KDDPDADAAHIIDWNVRAERRGWLPSAPQLNRNPFDVVSEAEATGTDPVAYTVEQLRT
                     GKLAIASEDPDHPK"
     gene            complement(2548..>3043)
                     /locus_tag="VN039_08225"
     CDS             complement(2548..>3043)
                     /locus_tag="VN039_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HWV45989.1"
                     /translation="YFRMDADFLRTGISPRARTTVELPVKAHHSTAGHIQKFPLLRWD
                     QMGQVVARETLPEFLQAEAMIEATDVSGMRTFAVKVQDDSMEPLFSKEEMIFVNPDLE
                     WKHDDYVIAHHRDGDSEAVLLRQVKRIGTHCILHPLNRKYADIPLTSQDAVWGKVVRL
                     RKNL"
BASE COUNT          624 a          912 c          877 g          630 t
ORIGIN      
        1 tcttggggtg atcgggatct tccgaggcga tcgcgagctt gccggttctg agctgttcga
       61 cggtatacgc gaccggatct gttcccgtcg cctccgcttc gctcaccaca tcgaacggat
      121 ttctgttgag ttggggcgcg gaagggagcc aacctctgcg ttcggcccgc acgttccaat
      181 caatgatatg ggccgcatcc gcatccggat cgtctttggc cgtcggcgag agaatctcgg
      241 ccggccgcag gctctcatac cgccactgat cggtatgggc gtaaaagaaa gaggtgccgt
      301 tcatgtgccg aggtgggcgg acccaatcga gcgcaaacgc cacggtcgtc cagccggcaa
      361 gcggccgcac tttttcttgc ccgacataat gagcccagcc gccgcccgat cgcccgacgc
      421 atccgcacat gacgaggacg ttgatgattg cgcggtagat catgtcggtg tggtaccact
      481 ggttgatccc gtttccgagg atgaccatcg agcggcctcg tgtcttttca gcgttctcgg
      541 cgaactcacg ggccagacga atgatctgac ggcgatggac acccgtatgt ttttcggccc
      601 atgccggcgt atagggaatg tcctggtcgt agtccttcgc cgcctgatcg tctccaagcc
      661 ctcgatcgag gccgtaactg gccagcaaga gatcataggc ggtgaccatg agctggtcgc
      721 cgtgaacggt gctgagccgt cgggccggaa tcaggcggcg cagaatctgc gggtgatcgt
      781 ctgaaaaata ggggaaggcg accgacacga cttcctcggc ttgcggcagc acgcttagcg
      841 cgggcgtaat ccgatgtccg gtcgtcgcgt cgatcagatc gagattccag cgtcctttct
      901 cacgccaccg aaacccgata ctgccctgag gggcgtacag atggccggtg gcctcgtcaa
      961 cagccaccgt cttccattcc ggattgttgg tttcacccaa ttggccatcg aagtcggatg
     1021 ctcggacaaa gcggtccggt gcatatccgt cgccggcggg acgcaggaag accaacatgg
     1081 gcatatcgct gtactggcgg caataatcca agaagtaggg actgcgcgcg gtcagatgaa
     1141 actccttcag gatgacatgg ccgaccgcca tggccagcgc ggcatccgtg cctcgccggg
     1201 gcgccaacca gagatccgcg aatttcacgt actccgcaaa atccggagcc acggcgacgg
     1261 ttttggcgcc cttgtatcgc gcagctgtgt aaaaatgcgc atcgggagtc cgagtctgcg
     1321 gaatgttcga cccccagacg acaatgtaag aggagttgta ccaatcggca gcctcgcaca
     1381 cgtcggtctg ctcaccccag acctgcggcg aggacggagg caagtcggca taccagtcgt
     1441 aaaacgacgg gatgacgcct ccgatcaacg acagatatcg gctgcccgcc gcaaaactca
     1501 ccatcgacat cgctgggatg ggagagaacc cggcaatgcg gtcgggaccg taccgctgga
     1561 tcgtatagac attggccgcc acgattagtt ccgtgacctc gtcccacgtc gcgcgcacga
     1621 acccgccctg tcctcgtatc gacgtgtact gctcgcgctt gtccgtgtct tcgacaattg
     1681 atgcccacgc ctctaccggc tccaacgtct tccgagcttc tcgccacaag gccatcaacc
     1741 gtgcccgcac caaggggtac ttaatgcgtt gcgcactgta gacgtaccag gaatagctcg
     1801 cccccctggg acatccgcga ggttcatgat tcggcaggtc ggggcgcgtc cggggatagt
     1861 ccgtctgttg cgtctcccag gtaatcaatc cgttctttac atacattttc caactacatg
     1921 agccggtgca gttgacccca tgtgtggaac gcaccacctt gtcatgattc cagcgatcgc
     1981 gataagcatt ctcccaggtg cgatcctctc gcctgacgac cccgtgtttc cctgaaaacg
     2041 tgtcgaggtt ctcacggaaa aacaggagcc gatcgaggaa atgactcata gacccttccc
     2101 gcaatacgcc tcaggccgga tgttcctgat ccacgaagcg gacgaaaccc ttcgtccaac
     2161 tacgccaaca tggtcgaatg cggcgatgac gaacacgtcc gcctcgcgag aagccacgtg
     2221 ccgatggttg cctgttgagt gaaacaaaac tcatcactcg aacacaccta gcaaaacacc
     2281 tagcgcgcat tgttcacggc gggtcatctc tgaaaggaag cattgcgccg gtccatgttc
     2341 aaagaatcgg gaggctgttc ctagttccgc cggagttccc cctctggaag gacggagatc
     2401 agggcaaagt ccagcgcctt gtggtagacg agcgcagctt gaagtttggg ggtgctctgg
     2461 atctccctgt tgctgaagga cattggtcga gagtccctac accgacagaa tgacgcgtta
     2521 ctctctttct gacggattag cgtacattta gagattcttt ctgagccgga cgaccttccc
     2581 ccacacggcg tcctggctcg tcagggggat gtcggcatac ttccggttca acggatggag
     2641 aatgcagtgg gtgccgatgc gtttcacttg gcgaaggagc acggcttccg agtctccatc
     2701 ccgatggtgg gcgattacat agtcgtcatg tttccactcg aggtccggat tgacaaaaat
     2761 catttcctct ttgctgaaca ggggctccat cgaatcgtct tggactttca cggcgaaggt
     2821 gcgcatcccc gacacgtcgg tcgcctcgat catcgcctcg gcttggagaa actcggggag
     2881 ggtttccctt gctacgacct gacccatttg gtcccagcgc aggagcggaa atttttggat
     2941 gtgaccagcc gtggaatgat gagccttgac cggcaactca accgtcgttc gtgcgcgcgg
     3001 cgatatgccg gtgcgcaaga agtccgcgtc catccgaaaa tac
//
LOCUS       DATMTI010000510         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_316703, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000510 DATMTI010000000
VERSION     DATMTI010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316703"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VN039_08230"
     CDS             complement(<1..1210)
                     /locus_tag="VN039_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV45990.1"
                     /translation="MKSLLRVLLYLRPHRALASATLVCAACATAMELVPPWVIKIIID
                     DVIQAKQASLLPWAIGLLVGAHVLKNLFASLRIRLNNQLEQTVVHDLRRHIFSALQRL
                     SITYFENRSTGEIMSRVTNDTEHVERIFIDGLEGMLTASLTLIGITGLLFMLNWKLAA
                     LSLLPIPLLALSASWFTSKVHGYYRETRQSAAELNGYLQDALSGIRETMGFDRQGHEQ
                     ARFDRLSLTYSDKNLKAMVLWSMYSPGMILLGALGTVLILWYGAGEVMEGRLTLGELV
                     LFLSYLAMFYVPINQIHSVNHMLQHALAASERVFDVLDTIPEVADRPYAVVPVHRAHG
                     AVRFDEVQFHYRPDVPVLRRFSVAVPAGERVALVGMSGAGKSTLLKLLIRFYDVTEGA
                     IIIDEKDVRDL"
     gene            complement(1233..1778)
                     /gene="def"
                     /locus_tag="VN039_08235"
     CDS             complement(1233..1778)
                     /gene="def"
                     /locus_tag="VN039_08235"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWV45991.1"
                     /translation="MRLKSETMLKIAKLGNPILRKVAAPIDPREIKSSEIQRLIDDMF
                     ETMYDEPGIGLAAPQVSRSIQLVVMGCNGEGGFPETVLINPSIVYYGPDQVETWEGCL
                     SVDGLRGKVTRPSLVRVKGLDRKGKPLDFEATELYAICIQHELDHLIGKVFLDRMTDM
                     STLTQLDEFAQYWKKEPTNVI"
     gene            complement(1827..2621)
                     /locus_tag="VN039_08240"
     CDS             complement(1827..2621)
                     /locus_tag="VN039_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV45992.1"
                     /translation="MRQSFLFRLGMIWMLVLMGGAEPSYAWADQVQVRQVQMPYEEPP
                     KGQDAPDVAIPPNKNPLSPEELKRTEALLPLLEGKQEFWAMGEFVHLGEPAVPVLIQA
                     LTMPSPRIRYNAIETLLMMKGVAGVPALVTTAKEPGEIPRVREHALRVAVRLDSAKAS
                     EAIEAMAKDPNPSVRKAAAFEARYVRQKAVIPILIPMVSDDERFVALSALQSLWILTR
                     HETEFHDWDTSTKQDRATWSNEWIEWWDTNKEIFEIPEPQRPKRSS"
     gene            complement(2668..>3688)
                     /locus_tag="VN039_08245"
     CDS             complement(2668..>3688)
                     /locus_tag="VN039_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV45993.1"
                     /translation="EIESVMKTVFGVKDLGIFRLTGQPNLLIQVDREASARYGLRVSD
                     VNAVVQAAIGGQAVTQVYEGEKLFDLVVRFLPEYRQDIDSIGNILVSTPDGARIPLKQ
                     VAAITMQTGAFIIYRENNQRYIPIKFSVRDRDLQSTIEEAQEKLAQHVTLPDGYHMEW
                     AGQYDQLKEEQKRLATIVPVSFVIMLLLLYTTFDSLKNALLVLAAVPFALVGGVLSLV
                     ITHTHFSISAAVGMISTLGVAILGGVLLISRIEEFRRAGLELRQAVIRGVDVQMRPIL
                     MATLSAAIGLLPASLATGIGAQAQQPLARVVVGGMLTAAFLILVVLPVLYEIVHQRDN
                     LKEQE"
BASE COUNT          847 a         1100 c          947 g          794 t
ORIGIN      
        1 acaagtcccg aacatccttt tcatcgataa tgatcgcccc ttcagtgacg tcgtaaaacc
       61 gtatcaacag cttcaggagt gtgctctttc ccgccccgct catcccgacc aatgcaactc
      121 gttctcctgc cggtacggcc actgaaaatc gcctcagtac cggaacgtca ggtcgatagt
      181 ggaactggac ctcgtcgaat cgaactgctc catgggcccg atggaccgga acaactgcat
      241 agggccgatc ggcgacttcc ggaatcgtat ccagcacatc aaagacccgc tcactcgccg
      301 ccaacgcatg ctgcaacata tgattgaccg aatggatttg attgatgggg acgtagaaca
      361 tcgccagata ggacaggaac aacaccaact cgccgagtgt gagacggccc tccatgacct
      421 cccccgcccc gtaccagagg atcaagacgg tcccgagggc tccaagtaaa atcatccccg
      481 gcgaatacat cgaccacaga accatcgctt tgaggttctt gtcactatat gtcaggctca
      541 gccgatcgaa acgcgcttgt tcatgccctt gacggtcgaa tcccatcgtc tccctgattc
      601 cggacaaggc atcttgtaga tagccgttga gctcagcggc gctctggcgg gtctcccgat
      661 aatacccatg gaccttcgac gtaaaccaac tcgcggacaa agcaagcaat gggatcggca
      721 agagcgagag ggccgccagc ttccaattga gcatgaacaa gagtcccgtg atcccgataa
      781 gggtcagcga cgccgtcagc atcccttcga gcccatcgat gaagatccgc tcgacatgct
      841 ccgtgtcgtt tgtcacccgg gacatgatct cccccgtcga tcgattctca aaataggtga
      901 tggacagacg ttggagggcg gagaagatat gccggcgaag atcgtggacc acggtctgtt
      961 caagttgatt attcagccgg attctgagcg aggcaaacag gttcttgagc acatgggcgc
     1021 cgaccaagag accgatcgcc catggcagca atgatgcctg cttcgcctgg atcacatcgt
     1081 cgatgatgat cttgatgacc cagggaggca ccagctccat cgccgtcgcg caagcggcgc
     1141 agacgagcgt cgcgctcgca agagcgcggt ggggtcgtag atacagcaag acgcgaagaa
     1201 gggacttcac aaccggctcg acaaggcggg cattaaatta cgtttgtggg ctcttttttc
     1261 caatactgcg caaattcgtc aagctgagtg agggtggaca tatcggtcat ccgatcgaga
     1321 aagactttgc cgatcaagtg atcgagttcg tgctgaatac agatcgcgta cagttctgtc
     1381 gcctcgaaat ccagcggttt gcccttccga tccagcccct tgacccgtac cagtgagggt
     1441 ctggtcacct tgcccctcaa tccatcgacg ctcagacacc cttcccaggt ttccacctgg
     1501 tcgggcccgt aatacacgat cgaagggttg atgaggaccg tttcgggaaa tccgccttca
     1561 ccgttgcagc ccatcacaac caattgaatc gaccgtgaga cttggggagc ggccagcccg
     1621 atacccggtt cgtcatacat cgtttcaaac atgtcgtcga tcagccgttg gatctcggac
     1681 gatttgatct cacgagggtc gatcggagcg gcaactttac gaaggatagg attacccagc
     1741 ttggcgatct tcagcatcgt ttcgctcttt aatctcatgt tatatcattc gtaacatagg
     1801 aatatccgag ctcgcaagct ccccggctaa gaactccgct tcggccgttg cggttctgga
     1861 atttcaaaga tctccttgtt ggtgtcccac cactcgatcc attcgttcga ccacgtcgca
     1921 cgatcctgtt tggtcgatgt gtcccaatcg tggaactcgg tctcatggcg ggtcagaatc
     1981 cagagtgatt gcaatgccga tagcgccacg aaacgctcgt cgtcgctcac catcggaatc
     2041 aagatgggga tgacggcctt ctgccggaca taccgcgctt cgaatgcagc ggcctttctg
     2101 acggatggat tgggatcttt tgccatggct tcaatcgctt cggacgcttt cgccgaatcc
     2161 agacgaacgg cgacgcgcag ggcatgttcc cgcacccgag gaatttcgcc gggttccttt
     2221 gcagtggtga cgagagcggg cactccggcg actcccttca tcatcagtaa ggtttcaatg
     2281 gcgttgtacc gaatccgcgg gctcggcatg gtcagtgcct gaataagaac gggaacagca
     2341 ggctctccca aatgtacgaa ttcccccatc gcccagaatt cctgcttccc ttcaagcaac
     2401 ggcaacaagg cttccgtgcg cttcagctct tcaggactga gcgggttctt attgggagga
     2461 atcgcaacat ccggcgcatc ctgccccttc ggcggctctt catagggcat ttgaacttgc
     2521 cggacctgga cttgatccgc ccatgcgtac gatggttccg ctccacccat caggacaagc
     2581 atccagatca ttcccagtct gaataggaaa gattgcctca cgatgatggc gctcctttct
     2641 ttattgtcca atgcatccgt caactgccta ttcctgttcc ttaagattat cacgctgatg
     2701 cacgatttcg tagagaaccg gcaacaccac gagaatcaag aaagccgccg tgagcatccc
     2761 tccaaccacc acgcgtgcca acggctgctg tgcttgagcg ccgatgccgg tcgccagaga
     2821 agcgggcaat aggccgatag ccgcgctcaa ggttgccatc agaatgggac gcatctggac
     2881 atcgacgccg cgtatcacgg cctgccgcaa ctcaagcccc gcccttcgga actcttcaat
     2941 gcgcgagatc agcaatacgc cccccaatat cgcgacgccc aaggtcgaga tcattcccac
     3001 ggcggctgaa atactgaaat gggtatgagt gatcaccaac gacaggacgc cacccactaa
     3061 cgcaaagggc acagccgcca acaccagcaa cgcattcttc aatgagtcga atgttgtata
     3121 gagcaacaac agcatgatga cgaagctcac gggaacgatc gtggccagcc gcttctgttc
     3181 ttccttaagt tgatcgtact gcccggccca ttccatgtgg tagccgtcgg gcagcgtgac
     3241 gtgctgcgcc aatttctcct gagcttcctc tatcgtactc tgaagatcgc gatcccgcac
     3301 actgaatttg atggggatat accgctggtt gttttcgcgg tagatgatga aggctccggt
     3361 ctgcatggta atggccgcca cctgtttgag cggaatgcgc gccccatccg gagtgctcac
     3421 cagaatatta ccgatggaat cgatatcctg acgatattct ggaagaaacc tgacgaccag
     3481 atcgaacagc ttctctccct catacacctg ggtcaccgct tgaccgccga tggcggcctg
     3541 aacgaccgca ttcacgtcgg agacccgcaa tccataacgg gcgctcgctt cgcgatcgac
     3601 ttgaatcagc aaattcggtt gaccggtcag ccggaagatg cccaagtcct tcacgccgaa
     3661 aaccgttttc attaccgatt cgatctcg
//
LOCUS       DATMTI010000511         1845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_826242, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000511 DATMTI010000000
VERSION     DATMTI010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826242"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1845
                     /locus_tag="VN039_08250"
     CDS             <1..>1845
                     /locus_tag="VN039_08250"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45994.1"
                     /translation="CALLFGALAGGGANAQSIVQPPLANVIDANGVNLTTGKFNLPGL
                     DVGIGSGQSGLSRVTQGDSDNLSGILSTSTTTAQFVLPPATANDILFYVTASYGGVSH
                     RFLLAHYYPPSNTWNYSAYPLPELGGGSAVLTCDGAQSDVISNRSGSCTLILEDGTRV
                     LYDRSLSSGASFGPMTSVTKPNGEVITFNYYTVTTNGVTTAKAVNTVNSSLGWALKYT
                     VDANYAVTGVTAVNTSAVRCDAVVCSPGGSYPTVTKSVNGATTTLSRNGVNMLSYSVS
                     GGVTTLTSPNGVSKTINTYTSGSFNGRVSSVVAGGQTWGYAYSSDGNTGETTTTVTEP
                     NGKTHKIVISYNSTIKSTTDEAGRTTTYLYDSNFHLVKTISPDGNALTGGFTAIAYDS
                     IGRVTSQQVVAKGGASNGVPNASASLVTSYAYANCDGTNDSWCRKPTSVTNENGVTTS
                     YTYDNASGQVATMMLPAPSSGGARPKVTYSFTNYSPQNYDVPGYEGSLHTVLNPIRLV
                     TGISTCRTADNGNCVNGADEKQTLISYAGSNNLLATSTTVKIGDGSYPQTTTATYDNN
                     GHVIVADGPKSGAVDETYTFYDTLGRVQGAVGVDPDGASGSRHRQASH"
BASE COUNT          415 a          512 c          520 g          398 t
ORIGIN      
        1 tgtgcgccct gctgtttggc gccctggcgg gcgggggcgc aaacgctcaa tcaatcgtgc
       61 agcctcctct ggccaatgta attgatgcaa acggcgtcaa tcttaccacg ggtaaattta
      121 atcttcctgg cttagatgtc gggatcggca gtgggcaaag cggcctttca cgggtgacgc
      181 aaggcgacag cgataatttg tctgggatac tgtccacgtc cacgacgacc gctcaattcg
      241 tccttccccc ggcaaccgcc aacgatattt tattttacgt tacagccagt tatggcggcg
      301 tgtcgcatag attcttgctg gcacattatt atcctccctc caatacgtgg aattacagcg
      361 cctatccgtt acccgaactt ggcggcggtt cggctgtctt gacgtgtgac ggcgcgcaat
      421 ccgatgtgat ttcaaatcgg tctggttcgt gtacgctgat actcgaggac ggaacccggg
      481 ttctctatga ccgcagcctt tcgtcggggg cgtcgtttgg ccctatgact tctgtgacta
      541 agccgaatgg cgaggtcatt acgttcaact actataccgt gacaacgaat ggcgtgacga
      601 cggccaaggc cgtcaatact gtcaacagtt cattgggttg ggcgcttaaa tataccgtcg
      661 acgccaatta tgcggtgacc ggcgtcacgg cggtgaacac atccgctgtt cgttgtgacg
      721 ctgtcgtttg cagtccgggc ggcagctatc ctacggtgac gaagtctgtc aatggggcca
      781 caacgacgtt gtcgcgaaac ggcgtcaaca tgttgagcta ctcagtgtct ggaggtgtta
      841 cgaccctgac atcgcctaac ggcgtttcaa agacgatcaa cacttacaca tccggatcgt
      901 ttaatggtcg agtttcttcg gtcgtcgccg gggggcagac ctggggctat gcctatagct
      961 cagacggaaa tacaggtgaa acgaccacca cggtgacgga gccgaacggg aagacacata
     1021 aaatcgtcat ctcctacaac agtacgatta agtcgacgac ggacgaggcg ggccggacaa
     1081 ccacttacct gtacgacagt aacttccacc tcgtaaaaac gataagcccg gacggtaatg
     1141 cattgacggg cggctttacg gcgattgcct atgattccat cgggcgcgtt acaagccagc
     1201 aagtcgttgc aaaaggaggg gcaagcaacg gcgttcctaa tgccagcgcc tcacttgtca
     1261 ccagttatgc ttatgccaat tgcgacggga cgaacgacag ttggtgtcgc aagccgacct
     1321 ccgtcaccaa tgagaatggc gtgacgacaa gctacaccta cgataacgca agcgggcaag
     1381 tcgcgacgat gatgcttccg gcgccgagtt caggtggcgc gcgaccgaag gtcacgtaca
     1441 gctttaccaa ttattccccg caaaactacg atgtgcccgg ctatgagggc agtcttcaca
     1501 cggttctcaa tcccattcgt ctggtcacag gcatctcgac ctgtcggact gcggacaatg
     1561 gcaattgcgt caatggcgcc gacgagaagc agaccctgat cagctatgcg ggcagcaaca
     1621 acctgcttgc gacctcgacg acggtgaaga tcggcgacgg aagctatccg cagacgacga
     1681 ctgcgaccta tgacaataac gggcatgtca tcgtcgctga cgggcccaag tccggcgcgg
     1741 tcgacgagac ttacaccttc tatgatacgt taggacgggt tcagggtgcg gtcggcgttg
     1801 atccggacgg cgcttcgggt tcgcgccatc ggcaggccag tcaca
//
LOCUS       DATMTI010000512         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_443501, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000512 DATMTI010000000
VERSION     DATMTI010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443501"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VN039_08255"
     CDS             <1..523
                     /locus_tag="VN039_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45995.1"
                     /translation="AEKALKDAQESGAATYSADEYAKLEGTLSAIRKEVADQDGKFTL
                     FRDYSKAQQLSASAKTDSERIKAASSQKKEEAKAAALQAQQVAEEAVKATQDLVAKAP
                     VGKDRAAVEAIKNDVEGLKSLLKQVQVSIDKEDYPAAQTQAKAIHDMSQGVSTEIQSA
                     LAKVGKGKLARKK"
     gene            532..1665
                     /locus_tag="VN039_08260"
     CDS             532..1665
                     /locus_tag="VN039_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWV45996.1"
                     /translation="MALDQTHKTLAVILCVLCLTGCVQAVPPDLLVALEAIDQDLIAL
                     RAPEAAPEEYGQFVHQWVSLKARVQLDDDLIRWPWESNDLENELRRLYIMGERTVSQL
                     HERQASQHRVAQAKVVRLEEELHTITSRVESIDGRILLGDKVVQTDLLVKQARSFFEQ
                     KDFQRAMQAAEQADHALKAQASLLSQELGRYADERRIAYWQTLAKQTIAWSRIHQSTA
                     IVVSKADRELTLYKSGRKVLSYPVRLGFNGMLEKQFQGDGATPEGRYRVTDKRGQGQT
                     QFYRALDLDYPNAEDRRRFELAKRSGRIGPAKGIGGQIEIHGADNERLAQTLGCIMLD
                     NPNMAVLYEGVSVGTPVTIVGALTRENAVALALSELAQRKDKI"
     gene            1676..2332
                     /locus_tag="VN039_08265"
     CDS             1676..2332
                     /locus_tag="VN039_08265"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWV45997.1"
                     /translation="MRRIHLAVAAITLLLLLIVSALLVVSDPTPSRVASEDSQVVHQP
                     TAQDKAGLRTLQARYKVLSKQLSQLMPNQPYILVDTARNRLYVKHQEQVVLNAIASTG
                     SGTILDKPGEGHSQWIFDTPRGEFLVKSKLTNPTWIKPDWAFVEEGLAVPQKVADRAE
                     QGVLGEYALGFGKGYFIHGTLYTRLLGKNVTHGCIRLNDSDLKGVYKLARVGTPIMIF
                     "
     gene            2360..2998
                     /locus_tag="VN039_08270"
     CDS             2360..2998
                     /locus_tag="VN039_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45998.1"
                     /translation="MNLCAILLLLAFTTPSWGKDIEAFPDLNLNNPNELKEATRVLEE
                     ELKLAARPQTYLLIDLGGSTIHIKSRGIGLHQIPIIAWSAGSREDLKGIHRLVARPSV
                     ARRKIEPGAGAEQEPISLADMPADYVLSFRPSLSVTVVPSVAGDSLLQSAVLLGKVGW
                     RNVKDWTSRLLTDHSSDPTSHLRLTLSVDHAQSLAWSLVDGMALVICRPNDK"
     gene            3106..3447
                     /locus_tag="VN039_08275"
     CDS             3106..3447
                     /locus_tag="VN039_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV45999.1"
                     /translation="MRLNDKLPPSFQSIVKPAFETLSVQVVDRLWAKDRMTPDIPIPG
                     RPFSFGTLAKAQAAGDMESLSAHDRHAVRVQLGRDQAATVNAITAALAGTSPSRGRRV
                     TLKERPKRSQR"
     gene            3449..>4044
                     /gene="pgl"
                     /locus_tag="VN039_08280"
     CDS             3449..>4044
                     /gene="pgl"
                     /locus_tag="VN039_08280"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWV46000.1"
                     /translation="MPVTPEIYVSRNVQEWATGATAFLLSVSEHAIQSTGRCLVALSG
                     GSTPKTLYRTLREPQWSSRFNWSKISFLFGDERCTPPEHPESNFGMAQIELFQPLNIR
                     PEHIYRMKGEYEDPTAAAHGYEETIRRVTRCPAPDPPCLDLILLGLGEDGHTASLFPG
                     TSALQEQSKIVTVGHAPTGIRSRLSLTLGVLNHAAVVL"
BASE COUNT          996 a         1102 c         1099 g          847 t
ORIGIN      
        1 agcggaaaag gctctcaaag atgcacaaga gagcggcgct gccacttata gcgctgatga
       61 gtatgccaag ctagaaggga ctctttccgc aatacggaaa gaagtcgccg atcaggatgg
      121 aaagttcaca ctctttcgag actatagcaa ggcacaacaa ctctctgctt cggccaaaac
      181 cgatagtgaa cggatcaaag cggcctcgtc tcaaaaaaag gaagaagcca aggcggcggc
      241 tttgcaagct caacaagtcg ctgaagaggc ggtgaaggcc acgcaagatc tggtcgccaa
      301 ggcaccggtc ggaaaagatc gtgcggcggt ggaggccatc aagaacgacg tggaagggct
      361 gaagtcgtta ctgaaacagg ttcaggtgtc gatcgacaaa gaagattatc cggccgcgca
      421 aacccaggcg aaggccattc acgacatgag ccaaggcgtg tcgacagaaa tacagagcgc
      481 tctcgcgaag gtgggaaaag gaaaacttgc aaggaaaaag taatacacgg catggcgctt
      541 gatcagacac ataagacgct tgccgtgatt ctctgcgtcc tctgcttgac cggttgcgtt
      601 caagccgttc cgccggatct ccttgtagcc cttgaggcca ttgaccaaga tttgatcgca
      661 ttgcgtgctc cggaagccgc acctgaggag tacgggcagt tcgttcacca atgggtctct
      721 ctgaaagcgc gcgtccaatt ggacgatgac ctgatcagat ggccatggga atcgaacgat
      781 cttgagaatg aactccggcg gctatacatc atgggcgaac gtactgtctc ccagctgcac
      841 gagagacaag cctcacagca tcgcgtggca caggccaagg tggttcgcct tgaagaagag
      901 cttcatacga ttacctcgag ggtcgaatcg atcgatgggc gtatcctcct aggagacaaa
      961 gtcgttcaaa cggatctcct tgtcaaacag gcccgctcct ttttcgaaca aaaagacttc
     1021 caacgagcca tgcaagcggc agagcaagcg gatcatgccc tcaaggctca ggcttcatta
     1081 ctcagccaag aattggggcg atacgccgac gagaggcgga tcgcctattg gcagaccttg
     1141 gccaagcaga ctatcgcttg gtcgcgaatc catcaatcaa cggccatcgt ggtgagcaag
     1201 gccgaccgag agctgactct ctacaagagc gggcggaagg tgctatcata tcccgtcagg
     1261 ttggggttca atggcatgct ggaaaaacag ttccaggggg acggagcaac accggaaggt
     1321 cggtatcgtg tgacggacaa gcgcggccaa gggcagacgc aattctaccg agccctggat
     1381 ctggattatc ccaatgccga ggatcgtcgg cggttcgagc ttgcgaaaag gtcgggaagg
     1441 atcggtccgg ccaaaggcat tgggggccaa atcgagatcc atggagccga caatgaacgg
     1501 ttggcccaga cccttgggtg catcatgctc gataatccaa atatggctgt tctctacgag
     1561 ggggtttccg tcgggacacc ggtcacgatc gtcggtgcct tgacgagaga aaatgcggtg
     1621 gctctggcct tatcggagct cgcacagcgc aaagacaaaa tctgagcata aactgatgcg
     1681 ccgaatccat cttgcagtcg cagcgatcac tttactcctc ctactgatcg tgagcgctct
     1741 actggtcgtg agtgacccga cgcccagtcg cgtggcgtcc gaagattccc aagtcgttca
     1801 ccagcccact gcccaagaca aagccggtct tcgtaccttg caagccaggt ataaggtgct
     1861 ctccaagcag ctgtcccaac tcatgccgaa ccagccttac atcttggtcg atacggccag
     1921 gaacagactc tatgtgaagc accaagagca agtcgtcctc aatgcgatcg cttcgaccgg
     1981 tagtggaaca attctcgata agcccggcga aggtcacagc cagtggattt tcgatacgcc
     2041 acgaggagaa tttctcgtca agtcgaaact gaccaatccc acgtggatca agcccgattg
     2101 ggcgttcgtc gaagaaggac tcgcggtgcc gcaaaaagtc gccgaccgtg cggagcaggg
     2161 ggtcctcggc gaatatgcgc tgggattcgg caaaggctat ttcatccatg ggacactcta
     2221 tacccgtttg ttgggaaaaa acgtgacgca tggatgcatc cggctcaatg acagcgatct
     2281 taaaggtgtc tacaagctcg ctcgagtagg gacaccgatc atgatctttt gatccctcaa
     2341 gagcgcgcag atattaccca tgaacctctg cgccattctt ctcctgcttg ccttcaccac
     2401 accaagttgg ggaaaggata tcgaagcctt tcccgacctg aatctcaaca accccaacga
     2461 actcaaagag gcaactcgcg tccttgagga agaactcaag ctggctgctc gtccccaaac
     2521 ctatctcctg attgatttgg gcgggagtac gattcacatt aaaagtcgtg gaatcggcct
     2581 ccaccagatt cccatcattg cctggtccgc cggatcacgg gaggatctga aagggatcca
     2641 ccgattggtt gcacgacctt cggtcgccag acgaaagatt gaacctggtg caggagcaga
     2701 gcaggagccg atttcactcg ctgatatgcc ggccgactac gtgttgtctt tcagaccctc
     2761 cctgagcgtc accgtagttc catcggttgc cggtgacagt ctccttcaaa gcgccgttct
     2821 actggggaaa gtggggtggc gaaatgtgaa agactggaca agcaggctct taacggatca
     2881 ctcatctgat cctacgtctc atcttcggct caccctctcg gtcgaccatg cgcaatccct
     2941 ggcctggtct ctggtcgacg gcatggcgtt ggtcatttgt cgcccaaacg ataaatagga
     3001 aaacagagca tagagtccct gttgcctttt ctaaaccggc tcaataagat acgaatgagt
     3061 tacccaccaa ctctgagatt ccgttgaaga aggaggcccc tgtctatgcg gttgaatgac
     3121 aagcttccac cgtcgtttca atccatagtg aaaccggcct tcgaaacgct cagcgtgcag
     3181 gtcgttgacc ggttatgggc aaaagacagg atgacgcccg acataccgat tcccggcagg
     3241 ccattctcct ttggaacatt ggcaaaagca caggcagccg gtgacatgga atcgctcagc
     3301 gcacacgatc gccatgcagt tcgtgttcag ttaggccgtg accaagccgc cacggtgaat
     3361 gccatcacag cggcgttggc agggacttca ccatctcgtg gtcggcgtgt gacattgaag
     3421 gagcgtccta agcgctctca acgttgatat gcccgtcacc ccggaaatct acgtctcgcg
     3481 caacgtgcag gaatgggcga cgggtgcgac ggctttcctc ctgtccgtta gcgagcatgc
     3541 gattcaatcc accggccggt gtctagtcgc actctccgga ggatctactc cgaagactct
     3601 ctatcgaacc ttacgcgaac cgcagtggag ctcgcgtttc aattggtcga aaatctcttt
     3661 cctatttgga gatgaacggt gcacgccacc tgaacatccc gagagcaatt ttggaatggc
     3721 tcaaattgaa ctgtttcagc cattgaacat tcggcctgag catatttatc ggatgaaggg
     3781 tgagtacgaa gacccgacgg ctgcggctca tggatacgag gaaacgattc gaagggttac
     3841 tcgatgtcct gctccagatc ctccttgcct agacttgatc cttcttggcc tcggcgagga
     3901 cggccatacc gcatcgcttt ttcctggaac ctctgctcta caggaacaat ccaagattgt
     3961 cacggttggt catgctccca caggcatcag atctcgtctt agcttgaccc taggtgtgct
     4021 gaatcatgca gctgtggtac tgtt
//
LOCUS       DATMTI010000513         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_613174, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000513 DATMTI010000000
VERSION     DATMTI010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613174"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..1290
                     /locus_tag="VN039_08285"
     CDS             364..1290
                     /locus_tag="VN039_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV46001.1"
                     /translation="MSAIISVADLAKTYASGFAALKHVNLDIDDGEILALLGPNGAGK
                     TTLISIICGIVTASSGKVTVAGHDIVRDYRAARSLIGLVPQELTTDAFETVWATVSHS
                     RGLFGKSASPAHIESVLRDLTLWDKKDNKIVELSGGMKRRVMIAKALSHEPKILFLDE
                     PTAGVDVELRKEMWGLVRRLRDSGVTIILTTHYIAEAEEMADRIGVLLNGELILVEEK
                     AELMRKLGKKQLMLDLEDRLSELPAALRNRNLSLERDGAQLVYTYDTQSERAGIERLL
                     TDLGQLGIGFKDLQTKESSLEEIFVDLVREAQ"
     gene            1287..2048
                     /locus_tag="VN039_08290"
     CDS             1287..2048
                     /locus_tag="VN039_08290"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV46002.1"
                     /translation="MNLVAVGAIYKFEMARTFRTWMQSIIAPVISTSLYFVVFGSAIG
                     GRITQIDGVSYGAFIVPGLIMLMILTQSVSNGSFGIFFPKFLGTIYELLSAPVSPLEI
                     VIGYVGAAASKSIILGLIVLVTAGVFVPIRIDHPIWMVSFLVLTAVTFSLVGFIIGIW
                     AKGMEQLQVVPLLIITPLTFLGGTFYSVGVLPPLWQTVSLFNPVLYLISGFRWAFYGI
                     SDVNVGISLSATLGFLLICLGIVAWIFRTGYRLKA"
     gene            2219..>2551
                     /locus_tag="VN039_08295"
     CDS             2219..>2551
                     /locus_tag="VN039_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HWV46003.1"
                     /translation="MAARAADKGITYVAFTATPKGKTLELFGRVPDPTQPASQTNVPE
                     PFHVYSMRQAIEEGCILDVLQNYTPYSLAFKLASGGEELDDTEVERSEAVKSLMRWVR
                     LHDYNISQK"
BASE COUNT          514 a          743 c          723 g          571 t
ORIGIN      
        1 ggaaagtgtg taggcgggta accgtctcaa gggttcgaat ccctttctct ccgccaccta
       61 gtctattgat tttgcatgac aatttggcaa gaacgctatt ctgggacttg tccgagactt
      121 ggccccggtt agcatcgcaa cgaccgcacc ttgagagcgt ggttcaccgg cttttgagag
      181 cctaattcag cgcctttctc aaagaccgat ttgcgtggtt cccttccctg tcgggccgga
      241 gggccgtaca gctgagttcg tggggctccc gatacagtct ggttgggtgg tctgaagggc
      301 acgggcttga caagaaccca ccctaagccg acctgcggga ccgacgccat tcccgagaag
      361 cctatgtccg cgatcatatc cgtagccgat ctcgccaaaa cctacgcctc cgggtttgca
      421 gcgctaaagc acgtaaatct cgacattgac gacggcgaaa tcctagcttt gctcggtccg
      481 aacggcgccg gcaaaaccac gctaatctca atcatctgtg ggatagtcac ggcctcatcg
      541 ggcaaagtta ccgtagccgg ccacgatatc gtgcgtgatt atcgtgccgc ccgctcactg
      601 attggcctgg tgccccagga gctgaccact gacgcttttg agacggtgtg ggcgacggtt
      661 tctcatagcc gcggcctgtt cggtaagagc gccagcccag cccatatcga aagcgtcctg
      721 agggacctca ccctgtggga taagaaggac aacaaaatcg tcgaactctc tggcggcatg
      781 aagcgccgag tgatgatcgc caaagcgctc agtcatgagc ccaagatcct attcctcgac
      841 gagccgacgg caggcgtcga tgttgaactg cgcaaggaga tgtgggggtt ggtgcgccgg
      901 ctgcgggatt cgggggtgac catcatcctc acaacgcact acatcgcgga ggctgaggag
      961 atggcggacc gtattggggt cttgctcaat ggtgagctga tcctggtcga agagaaagcc
     1021 gaactcatgc gcaaactggg caagaagcag ttgatgctcg acctcgagga tcgcctatcc
     1081 gagttgccgg cggcgctcag gaaccgcaat ctcagcctag aacgcgatgg cgcgcaactg
     1141 gtctatacct atgacaccca gagcgagcgt gccggcatcg agagactgct gactgacctc
     1201 ggccaacttg gaatcggctt caaggacctg cagactaaag agagttcgct cgaagaaatc
     1261 ttcgtcgacc tggtgaggga agcacaatga acctcgtcgc tgtgggggcc atttacaaat
     1321 tcgaaatggc gcggacgttt cggacctgga tgcagtcgat catcgctcca gtcatttcga
     1381 cctcgctcta cttcgtcgtc ttcggctcag ccatcggggg acggatcaca caaattgatg
     1441 gtgtcagcta cggcgcgttc atcgttcccg gcctcatcat gctcatgatc ttgacccaga
     1501 gcgtctcgaa cgggtccttc ggcatattct tcccaaagtt cctcggtaca atttatgaac
     1561 tgctttcagc tccagtctcg cccctcgaaa ttgtcatcgg ttacgttgga gcagcggcca
     1621 gcaagtcgat catcctaggc ctcatcgtgc ttgtcaccgc cggcgtcttc gtgccgatcc
     1681 ggatcgatca tccgatctgg atggtgagct tcttggtgct gaccgcggtc accttctcgc
     1741 tcgttggttt catcattggc atttgggcca aaggtatgga gcagcttcaa gtcgtgccgt
     1801 tgctcatcat tacgccactc acattcctcg ggggtacctt ctattcggta ggcgtgctgc
     1861 cgcccctttg gcagacggtg tcattgttca accccgtact atatttgatc agcggcttcc
     1921 gctgggcgtt ttacggcatt tccgacgtga acgtcgggat cagcctcagc gcgacgctgg
     1981 gctttctgtt gatctgcctg ggcatcgtgg cttggatctt ccggacggga tatcggctaa
     2041 aggcttagcg gctggtgagc cggcattttg aaagctcacc tacacgctgc tctcgatcca
     2101 agcggttact gtttgtaacc gaattgagag ttcgcatcga ttattctccc ccacctagtc
     2161 tgctgatttc ggtagcgatg gcggcgaggt cagcaccgag gatctacttg cggctcagat
     2221 ggccgcccgt gcggccgaca agggcatcac ctatgtggcc ttcactgcaa ccccgaaagg
     2281 caagacgctg gagctgttcg ggcgcgttcc cgacccgacg cagcccgcca gccagacgaa
     2341 cgtccctgag cccttccacg tctactcgat gcggcaggcc attgaggagg gctgcatcct
     2401 cgacgtgctt cagaactaca cgccctatag cctcgccttc aaactcgcga gcggcggaga
     2461 ggagttggac gataccgagg ttgagcgctc cgaggccgtg aagtctctaa tgcgctgggt
     2521 tcgcctccac gactacaaca tcagccagaa g
//
LOCUS       DATMTI010000514         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_826267, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000514 DATMTI010000000
VERSION     DATMTI010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826267"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            559..906
                     /locus_tag="VN039_08300"
     CDS             559..906
                     /locus_tag="VN039_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46004.1"
                     /translation="MSLTALRQRLRFCLTIIHSRIVILATAIADMKEPYDLDVAPNGK
                     IFVSQCGWGRRFNNMDDALGYINKMERLDEEAGSGLARNMFGILGACIRRPFSRSSHP
                     LGVEPCSSGRVEA"
     gene            1529..1783
                     /locus_tag="VN039_08305"
     CDS             1529..1783
                     /locus_tag="VN039_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46005.1"
                     /translation="MTITINETDFELFEHLDFEHVTPCDGEGCGEEAKWRAVATCCGR
                     VILACEECHQTLVNAQNVFGVLIDYACVGEPVMRLSWYKL"
BASE COUNT          498 a          447 c          513 g          484 t
ORIGIN      
        1 cttagtgacc caccccgttg taacgctaca ccagatgtgg cccgggttaa cggtgggcaa
       61 tacaatagtc cattcggtca ccgactatgg cccgggtgga ctattttact cccctggcct
      121 tgaccgacca ggtaaattag ggtagagtta tcttgaaacg ggcaatagtg ccccactaac
      181 tcatgggaga aaatatgtca aacaagccat ctagaagggc tgttgttcga accggcgtct
      241 ggtcggtacc ggcggtagcg attgcgacag cagcaccggc tgccgcgaat cattcaggtc
      301 cagtgaccat tccattgtcg agcgcaatct attggaagtg cccgggcaag gctaggggtc
      361 gcgtgaccaa ctatccgaag accacaatct tcactcttgc tttcgggcag gtcgtcaata
      421 cgcttctggt gactgatatc gagttccgag gagtccagca caaggtgaag gacatagttc
      481 acgactgcga ccccaaggtc tggcacgtaa ttttcgagac cgaccaaaac tctcgtcatg
      541 gtgatggagt cctctcctat gtcgctgacg gcattgcggc aacgactccg gttctgcctg
      601 acaatcatcc attcccgcat cgttatcctt gctacggcga ttgctgacat gaaagagcca
      661 tatgaccttg atgtggcccc gaatggcaag attttcgttt cgcaatgtgg ctgggggcgt
      721 cgtttcaaca atatggatga cgccctcggt tacatcaaca agatggagag attagatgaa
      781 gaggctggca gcggtcttgc tcgcaatatg tttggcattc tcggcgcctg cattcgcaga
      841 ccattctccc ggagtagtca cccattggga gttgaaccat gttcatcggg gagagtcgaa
      901 gcatgatgta caggcaacct tcggcaccag gggtcgttgg atagccgaat ggtatggacc
      961 tcagggcaac cagataaaga cctatcgtta tacagacaaa tctcaactgg tagaggcgca
     1021 ttatcgtaag cgagcattcg agtcgaaggg ttggcacctg tttaaagcct acctgattcc
     1081 gaatgacagg cattgaccct tgacgagtac atgtcagcat ggtacgatgg ttgtcatgct
     1141 gacatgtacc agatgtggaa gaactcgcct cgaaaagtac atcgttctgc gtaagacata
     1201 ctctggtaat aaaaagtatc gttgtcgagc acgttcggtg tgctcgtcca tcgccaggag
     1261 gaagaatgac cgtcgtaatg ggtgaaactg atacagatat catcgaatta ctagacttca
     1321 agttcgagga aacatgtgaa agtgttctcg gttgtgacaa ggctgccgcc tgggcactga
     1381 cactgctctg ctgcgaagaa atattctttc tttgtgagaa gtgtacagaa gacgttcgaa
     1441 aattggaggg cattctctac aatccgcagt gtaaattgtg taaaacagtg ctaccacgtg
     1501 gtagaattat taaaaggtac gacagattat gaccataact atcaatgaaa cagacttcga
     1561 actcttcgag catcttgatt tcgagcatgt tactccttgt gatggtgaag gatgtggcga
     1621 agaagcaaag tggagagcag tggcaacgtg ctgcggcaga gtcatcctgg cctgtgaaga
     1681 gtgtcatcaa accctggtga atgcccaaaa tgtgttcgga gtactaattg actatgcatg
     1741 tgttggcgag cccgtcatgc gattatcctg gtataaatta tgacgacatc agttcttgaa
     1801 acagatattg aaattgaccc atctgacatc attggtatgc cccagccgtg tgacgggctg
     1861 ccattcgata ctctttgtga gaacgaggcg gtatacaggt gtgaagtctc ttgctgtcat
     1921 catattctat tctggtgtga ga
//
LOCUS       DATMTI010000515         1964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_697835, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000515 DATMTI010000000
VERSION     DATMTI010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697835"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VN039_08310"
     CDS             complement(<1..958)
                     /locus_tag="VN039_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWV46006.1"
                     /translation="MLLESGVASSEEAAELATNLNGGSWVSQVLDSGKVDEQRFLGAI
                     GKFFRVPIVSMDAKNIDRSTLSILPSRFVFQHHILPIESKENSVVLATYDLFNSIGRQ
                     LAGQLLKKPAEWVLVPRAQLLRAMKSLYGVGAETFDEILKTRGSMDVLEDTETAIELN
                     ADDPEASVVKFVNQIIREAIAERATDIHVEPLENDLRIRYRIDGILHEVAVPPQLRVL
                     QSAIISRLKVMSHMDIAERRLPQDGRMNLLAGNVEIDVRVSTIPTVNGESISLRLLSR
                     GEQQFNFERLDLSDRQEKIIRNLLAQPNGIILLTGPTGCGKST"
     gene            complement(1002..1412)
                     /locus_tag="VN039_08315"
     CDS             complement(1002..1412)
                     /locus_tag="VN039_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HWV46007.1"
                     /translation="MKRITLVLVVGLGAMVARGDEFDIVDYGIFRSVDPLAQRDEWGT
                     THQTPHVIATDFAPRYSWRWMYYFKGRDQLVLDPAYVGALQTTLRRLGYYCGPVDGVF
                     TAEVSYAIARLQKNYSMRVTGTLTTPVRRALHLP"
     gene            complement(1425..>1964)
                     /locus_tag="VN039_08320"
     CDS             complement(1425..>1964)
                     /locus_tag="VN039_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HWV46008.1"
                     /translation="VASVSSSIEYKKVVLQLEVVPLINSEKEVSLDILQKIDSLVPNG
                     SVNISGNLVPTIDTKYIRTNVAAPNGSTIILGGLIQDTKQKNYQGFPYLSRIPLIGAA
                     FRSTAAGKHRQELIILMCPEVTLTKLDLYRLRQKWENKNTHFGPELDQAECPDCPRVE
                     TGKQINLPPPDLPDDKEMH"
BASE COUNT          449 a          528 c          516 g          471 t
ORIGIN      
        1 aggtcgattt gccgcagccg gtaggacccg tcagaaggat aattccgttt ggttgagcaa
       61 ggaggttgcg aatgattttc tcctgcctgt cgcttagatc gagtcgttcg aagttgaact
      121 gctgttcgcc gcggctaagt aaacgtaaac tgattgattc gccgttcacg gttgggatcg
      181 tggaaacgcg gacatcgatc tcgacgttgc ccgcgagcaa attcatcctg ccgtcctggg
      241 gaagccgtcg ctcggcaatg tccatgtgcg acattacctt cagccgggag atgattgccg
      301 attgcaaaac gcgcaactgc ggtggcaccg cgacctcatg caaaatgccg tcaatccggt
      361 accgaatacg caaatcattt tccagcggtt cgacgtgaat gtcagtggca cgctcagcga
      421 tcgcttcacg gatgatctgg ttaacgaatt tgaccaccga cgcttccgga tcgtcggcgt
      481 taagctcgat cgcggtctcc gtgtcttcca acacgtccat cgagcctctg gtctttaaga
      541 tctcgtcgaa cgtttccgcg ccgacgccat acagactttt catggcgcgc aggagttgcg
      601 cgcgcggcac gagcacccat tcggcaggtt tcttcagcag ttgccctgca agctggcgac
      661 cgatcgaatt gaaaagatca taggtcgcga gcacgaccga attttctttc gactcaatcg
      721 gcagaatgtg gtgttgaaaa acaaaacgac tcggcagaat cgacaaagta gatcgatcga
      781 tgtttttcgc gtccatggag acgatcggca ctcggaagaa cttgccgatt gcgccgagaa
      841 aacgttgctc gtccactttg cccgaatcga gcacctggct tacccacgaa cctccattga
      901 ggttagtcgc caactcggct gcctcttcgc tcgaagccac accgctttcg agcagcaaat
      961 taatcagtga ctgactcgga gctgcattca ttcaacggga cttaaggcag gtgtagcgct
     1021 cgccgcacgg gagtggtcag ggtaccagtc acgcgcatcg agtaattctt ttgcaggcgc
     1081 gcgatggcgt agctcacttc tgctgtgaac acgccgtcca ctggtccgca atagtagccg
     1141 aggcgacgca acgtcgtttg cagggctcca acataagctg gatccaaaac aagttgatcg
     1201 cggcccttga agtaatacat ccatcgccac gaatacctgg gagcaaaatc agttgcgatc
     1261 acgtgcggtg tttgatgagt ggttccccat tcgtctcttt gagccagagg atcgaccgat
     1321 cggaagatcc cataatcgac aatatcaaac tcgtcgcccc gtgcgaccat cgcgcccaac
     1381 ccgacaacca gaacgagtgt tattcgtttc ataaatgaaa aggcttaatg catctcttta
     1441 tcgtccggaa gatccggcgg cggcaagttt atctgtttgc ctgtctccac tctggggcag
     1501 tctggacact cggcctggtc gagttccgga ccaaaatgtg tgttcttgtt ctcccatttt
     1561 tggcgcaatc tgtaaaggtc gagcttcgtc agggtgacct ccgggcacat cagaataatc
     1621 agttcttgac gatgcttgcc agccgcggtg gaacgaaacg cagcaccgat gagaggaatg
     1681 cgcgagagat aagggaaccc ctgatagttc ttctgctttg tgtcttgaat caagccaccg
     1741 aggatgatcg tcgatccgtt cggagcagca acgttcgttc gaatatattt tgtgtcgatt
     1801 gttggaacca aattcccact gatgtttacg cttccgtttg gaacgaggct gtcgatcttt
     1861 tgcaggatat cgagggagac ttccttctcg gaattgataa gcgggaccac ctcaagctgt
     1921 aacactacct tcttgtactc gatgctcgat gataccgaag ccac
//
LOCUS       DATMTI010000516         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_273446, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000516 DATMTI010000000
VERSION     DATMTI010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273446"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1042)
                     /gene="argF"
                     /locus_tag="VN039_08325"
     CDS             complement(65..1042)
                     /gene="argF"
                     /locus_tag="VN039_08325"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWV46009.1"
                     /translation="MTVPARQRIRDGRYAKDLLDVATMPREQVFDLLRLATSLKKKQR
                     QGTPHRLLHGKTLGLLFQKPSTRTRVSFEAGMNQLGGHALVLPMSDIQLSRGETVSDT
                     ARVLSRYLDGIVIRTYDHAIVEEWAAEATMPVINGLTDHSHPCQALSDLMTIQEIKGR
                     LKGLSLAYVGDGNNVANSLIEAGTKVGLRIVIGCPSGYQPDQRVIDRARMEGQATGAS
                     IEVLENPLIAVKEADVVYTDVWISMGREREQARRLRTLAPYQLNKRLLQRAKPDAIVM
                     HCLPAHRGEEIAAEVLDGTQSVIIDQAENRLHMQKAILTQLLSRKKHTG"
     gene            complement(1042..>2162)
                     /locus_tag="VN039_08330"
     CDS             complement(1042..>2162)
                     /locus_tag="VN039_08330"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HWV46010.1"
                     /translation="VYDLEGREYIDFVGGIAVNVLGHGHPDLVQAIQRQAVQLIHTSN
                     LYYTEPQVKLAKMLVDYSFADRVFFCNSGAEANEAAIKLARRYGHERHGPDRFEVITM
                     KNSFHGRTLATLTATGQEKVQKGFEPLMPGFAYAPFNDFGAIESMVSEKTAAIMLEPI
                     QGEGGVYEADRDYLRNLRDLCTRQDILLIFDEIQTGMGRTGTLFAHEQFGVKPDIMTL
                     AKGLGGGVPIGACLAKESVAAAFTPGSHASTFGGNPLACAAGLAVCRALLEGKVLDQA
                     RHMGEYLAKGLADCKDRHRIVKDIRGLGLLQGMELEADARAVVADALARGVLINAAGE
                     RVLRFVPPLIISQEDIDKLLDVLSAIFNQRGTDKDSHH"
BASE COUNT          427 a          660 c          577 g          498 t
ORIGIN      
        1 gtatccagtc cgccggaata ggccaggacg attttcttga tcggtgttgg tttcatggtg
       61 gactctaccc ggtatgtttc ttccggctga gcaactgggt caaaatcgcc ttttgcatgt
      121 gcaggcgatt ttccgcttga tcgatgatga cggactgtgt gccgtccagg acctccgccg
      181 caatctcttc tccgcgatgg gccggcaaac aatgcatgac aatggcatcg ggtttcgctc
      241 gttgcagcag ccgtttgttc aactggtagg gagcgagcgt tcgtaatcgt cgcgcctgct
      301 cccgctcgcg gcccatactg atccacacgt cggtatacac cacatccgct tctttcacgg
      361 cgataagggg attctccaac acctcgatgg acgcgccggt ggcttgtcct tccattctcg
      421 cacgatcgat cacccgctga tccggctgat agccggatgg gcatccgatc acgatgcgca
      481 aacccacctt cgtgcctgct tcgatgagcg agttggccac gttgtttccg tcaccgacgt
      541 aggcgaggct gagtcccttg agccggccct tgatctcctg aatcgtcatc aaatcagaca
      601 aggcctgaca aggatgacta tggtcggtca gcccgttaat aaccggcatc gtggcttctg
      661 ccgcccattc ctcgacgatc gcatggtcgt aggttcggat cacgatccca tccaggtaac
      721 gggacaatac gcgcgccgta tccgagaccg tctccccgcg cgagagttgg atatcgctca
      781 tggggaggac cagcgcatgg ccgccgagtt ggttcatccc cgcctcgaac gacacccgag
      841 tccttgtcga cggtttttga aacaggagtc ctaatgtctt cccatgcaac aaccgatgcg
      901 gcgtgccctg tcgctgcttc ttcttcagag atgtcgctaa gcgcaacaag tcgaagacct
      961 gctctcgcgg catcgtcgcg acgtcgagca agtccttggc atagcgccca tcgcggattc
     1021 tctgtcgagc tggtacggtc atcagtggtg tgagtctttg tctgtgcctc gttggttaaa
     1081 aatggcgctc agcacatcca gcagtttgtc gatgtcctcc tgcgaaatga tcaatggtgg
     1141 cacaaagcga agcacacgct cacctgccgc attgatcagc acgccgcggg cgagtgcatc
     1201 ggccaccacc gcccgggcgt cggcctctaa ctccatgcct tgcaagagac caaggcctcg
     1261 gatatccttc acgattcgat ggcggtcttt acagtcggcc aatccctttg ccaagtactc
     1321 gcccatgtgt cgcgcttggt ccagcacctt tccttcgagc agcgcgcggc agacggccag
     1381 ccccgcggca caggccaagg ggttgccccc gaacgtcgac gcgtgagagc cgggcgtaaa
     1441 tgctgcggcc accgattctt ttgccagaca ggctccgatc ggcactcctc cgccgagacc
     1501 tttagcaagg gtcatgatat cgggttttac gccgaattgc tcatgggcga ataaggtgcc
     1561 ggttctcccc atgcccgttt gaatttcatc aaaaatgagc aagatgtctt gtcgtgtaca
     1621 gaggtcacgc aggtttctga gataatcccg gtcggcctca tacacgccgc cttccccctg
     1681 aattggttcc agcatgatcg ccgcggtctt ctcgctaacc atggactcga tcgcgccgaa
     1741 atcattgaac ggtgcataag caaatcctgg catcagcggc tcgaaaccct tctgaacctt
     1801 ctcctgcccc gtagcggtga gcgttgccag cgttcggcca tggaacgaat tcttcatcgt
     1861 gatgacttca aagcggtcgg gtccatgccg ctcatgtccg taccgccgcg caagcttaat
     1921 cgccgcctca ttagcttcgg ctccgctgtt gcagaagaac acacggtctg caaacgaata
     1981 gtccaccaac attttcgcca gtttcacttg aggttcagtg tagtagagat tcgaggtatg
     2041 gatcagttgt acggcttggc gctggatggc ctgaaccagg tcaggatgac catgccccag
     2101 cacattgacg gctatcccac cgacaaaatc gatgtactcc cggccttcca gatcgtagac
     2161 tt
//
LOCUS       DATMTI010000517         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_61351, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000517 DATMTI010000000
VERSION     DATMTI010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61351"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2196
                     /locus_tag="VN039_08335"
     CDS             <1..>2196
                     /locus_tag="VN039_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46011.1"
                     /translation="FHGLNSAVTKVKNAAGQIMTLSTSTSGSTSVLTVTDPAGNNYVY
                     KSLPNANASFNLLPIDLGTVILPGSPATTVTYEYTSGKLSEVDYNGVAHDVTGYDSAG
                     RANMGAMADGTQQATILYGSNSLGPTATITNALGHVSVYQYNNSAELLSITGNASPHC
                     AAAFASTTYDTNGNIQSATDNDGNTTNYIYAATGQLQQTIEAAGTAYARTTNYVWDTT
                     ANTNRILSVTVVGYSKTSYTYNASNRLASVSVQNLSGVGTSQQTLTTTYGYTYYTNGT
                     VASMVVTPPSVAGKLTYAYDTFGNLTSVINVLGQATTFGNYTVLGQPGTSTTANGDVT
                     NYSYDARSRLVSISHTHAGVTNTGSLTYDPASGQVSDVVTYDNEHTHNTFDADFKLTS
                     STWSGPSGATITTSYTYDAMSNVTSVTRTLQGSSSPSFSRHWDYDELGRVIAVRGNHG
                     QQVTLAYDLNGNVAQTTDATGRSTFTQYDALNRLRAITDPNAGATTMAHDMGDHVSQL
                     IDPRGLITTFAYDGLGQLWTQTSPDSGISHLAYDTSGRLTTLTRNSGAVTTYAYGTLN
                     RLASVSAGGQMRSYTYDSCSNGAGKLCGYADSSGSTAFAYTQEGLVAQKSVTNGLGTN
                     SENYAYDAVGRLTQIVNSLATVTYTWTDDQISKVNAQFSGQNINVANNVTYDALRHVT
                     GWTYGNGLQRSQTFDSDGRASALAVVSGSAVLQNLGYGWDNADRITNLT"
BASE COUNT          555 a          620 c          551 g          470 t
ORIGIN      
        1 tttttcacgg acttaacagc gcagttacca aagtaaagaa tgccgccggt cagattatga
       61 cgctgagcac atctacatcc ggcagcacaa gtgttcttac ggtgactgat ccggcgggaa
      121 ataattatgt ttacaagtcc ctgccaaatg cgaatgcgtc gttcaatctc ctcccgattg
      181 accttggtac tgtcatttta cctggctctc ccgcaaccac ggttacctac gaatatacga
      241 gcggcaaact atcggaagtc gactacaatg gcgtcgcgca tgacgtcact ggttacgata
      301 gtgctggacg cgccaacatg ggcgccatgg ctgatggcac acaacaggca accattttgt
      361 atggctcgaa cagcttaggg ccaacagcga caatcactaa cgcgctaggt cacgtttcgg
      421 tatatcaata caacaacagt gcggagctgc tttccattac cggaaacgcg tcaccacatt
      481 gtgccgctgc cttcgcaagc acaacctatg acacaaatgg caacatccaa tctgcgacgg
      541 ataacgacgg taacactacg aattatatct atgctgcgac agggcagctc caacagacta
      601 ttgaagctgc cggtaccgcc tacgcgcgta cgaccaacta cgtttgggat accacagcca
      661 acacgaaccg tatactttcg gtgacggttg ttggttactc aaagacctct tacacctaca
      721 acgcaagcaa tcgactggcc tcagtgtcgg tccagaatct ctcgggggtt ggaacatcgc
      781 agcagacctt aacgaccact tacggctata cctactacac gaatggcact gtcgcgagca
      841 tggtggtgac acctccctcc gttgccggca agctcactta tgcctacgat acctttggca
      901 atctaaccag cgtaattaac gtcttggggc aagccaccac ctttggcaac tacactgttc
      961 ttgggcagcc tggaaccagc acgacagcca atggtgatgt caccaactac agctatgatg
     1021 cgcgcagtcg cttggtgtcg atttcacata cgcacgcggg tgtcacaaat accggaagct
     1081 tgacttatga cccggcatcg ggacaagtct ccgatgtggt gacctacgac aacgaacaca
     1141 cacacaatac gttcgacgcc gacttcaagc tcacctcgtc gacgtggtct gggccgtcgg
     1201 gcgcgaccat tacaacgtcg tatacctatg atgcaatgag caatgtcaca agcgtgaccc
     1261 gtacgttgca aggcagcagt tcgccttctt tctctcgcca ctgggactat gacgagttgg
     1321 ggcgcgtcat cgcggttcga ggtaatcatg gtcagcaggt gaccttggct tacgatttga
     1381 atgggaacgt cgcacagaca acagatgcca caggtcgaag cacgttcact caatacgatg
     1441 cgctgaatcg tctgcgggcg attacagatc ctaatgcggg agctacgacg atggcccatg
     1501 acatgggcga tcatgtcagc cagctcattg acccgcgcgg cctgatcacc acttttgcct
     1561 acgatggcct aggtcagctc tggactcaaa caagtccgga ttcgggaata tcccaccttg
     1621 cttatgacac gtccgggcgc ttgaccacgt tgacccgcaa cagtggtgcc gtaacaacct
     1681 acgcttacgg aacgttaaat cggctggcat cggtgagtgc ggggggacaa atgcgaagct
     1741 acacttatga ctcttgctcc aacggtgccg gaaagctctg cgggtacgcc gactccagtg
     1801 gaagcacagc attcgcctat acccaagaag ggctggtcgc tcaaaaatct gtgaccaacg
     1861 ggctaggcac aaacagcgag aactacgcgt acgacgcggt ggggcgcctc actcaaattg
     1921 tcaactcatt ggctaccgtg acttacactt ggacagatga tcaaattagc aaggttaacg
     1981 cgcaatttag cgggcaaaac atcaatgtcg ccaacaacgt aacctatgac gccttgcgtc
     2041 acgtcaccgg atggacctac ggcaacggct tgcagagaag ccagacgttt gattcggatg
     2101 gccgcgctag cgctctagcg gtggtttcgg gtagcgccgt cttgcaaaat ttaggctatg
     2161 gatgggacaa cgccgatcgc atcaccaact tgacta
//
LOCUS       DATMTI010000518         1544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_103852, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000518 DATMTI010000000
VERSION     DATMTI010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103852"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="VN039_08340"
     CDS             <1..1394
                     /locus_tag="VN039_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HWV46012.1"
                     /translation="QYQIIVYLMGVIVGKSLNLEDLDEPIQKPYRKLQVDDLPIIDVP
                     ETLDYRKLLADYEAQHGRPLRPIQRRANAKHRVPDSLTCPRCQAPSSYLYANNGGKGQ
                     YQCKVCQCRFNHRNRFTKQAVFRCPHCKKTLEKIKERKEYNIYKCKNNACPFYQANLR
                     RMTKKERQQFQQDPQAFKVRYLFREFLFDFLPLASSSLIKPKVDLSRLAASPHVLGLV
                     LTYYVNFGMSSRETAAAMKDIHGVSISHQTVLNYVNSVALWIKPFVDRFPYELSGSFC
                     GDETYIRVKGRWHYLFFMFDAVKKIVLSYRVSPNRDTLSAIKAIDDVLRKLASLPDDL
                     SFVVDGNPIYLLAQHFFAQHGISFDVRQVIGLTNEDPVSEEFRPLKQIIERFNRTFKG
                     NYRPTHGFGAEEGSVSFVTLFVAYFNFLRPHSALEGRVPVVIPELADLPHMPARWTKL
                     IAMAQAFLQQEAA"
BASE COUNT          334 a          450 c          400 g          360 t
ORIGIN      
        1 cccaatatca aatcattgtg tatttgatgg gtgtgatcgt gggaaaatcc ttgaatctcg
       61 aggacttgga cgaacccatc caaaagccgt accggaaact tcaggtcgat gaccttccga
      121 tcatcgatgt gccggaaacc cttgattacc gaaagctgtt ggcagactat gaagcccagc
      181 acggacgtcc tttacggccc atccagcgcc gcgcgaatgc gaaacaccgt gttccggatt
      241 cgttgacctg ccctcgctgc caggctccct cttcttacct gtacgccaat aacggcggaa
      301 aagggcaata ccaatgcaaa gtgtgccagt gccggttcaa ccaccgaaat cggttcacca
      361 agcaagcggt ctttcgctgc ccgcactgca aaaagacgct ggaaaaaatc aaggaacgca
      421 aagaatacaa catctataag tgcaagaaca acgcttgccc gttttaccag gccaaccttc
      481 gccggatgac gaagaaggag cgccaacagt ttcaacagga tcctcaagcc ttcaaggtgc
      541 ggtatttgtt tcgagagttc ttgtttgact ttctcccgtt ggcttcctcg tcgctcatca
      601 agccgaaggt cgatttgtcc cggctggctg cgtcgccgca cgtgttgggg ctcgtgttga
      661 cgtactacgt caactttggg atgtcctcga gggagaccgc cgccgccatg aaggacatcc
      721 atggcgtctc gatctcccat cagacggtgc tcaactacgt caacagcgtc gcgctttgga
      781 tcaagccctt tgtcgaccgg ttcccgtatg agctgtccgg ttcgttttgc ggggacgaga
      841 cgtatattcg cgtcaaaggg cgctggcatt acctgttctt tatgtttgac gccgtcaaaa
      901 agatcgtgct gtcgtatcgc gtctcgccca accgggacac gctctcggcc atcaaggcca
      961 tcgatgatgt cctcaggaag ctagcgtccc tcccagacga cttgtcgttc gtcgtggatg
     1021 gcaacccgat ttatctgctg gcgcaacact tcttcgccca acacgggatt tcatttgacg
     1081 tccgccaagt gatcgggctg accaatgagg atccggtctc ggaagagttt cgccctctca
     1141 agcagatcat cgagcgattc aaccggacct ttaaaggcaa ctaccgcccg acccatgggt
     1201 ttggtgcgga agaaggctcg gtgtcctttg tgacgctgtt tgtggcgtat ttcaactttc
     1261 tgcgcccaca cagcgcgctc gaaggccggg tgccggtcgt catcccggaa ctggcggatc
     1321 ttccgcatat gccggcccgg tggacgaagc tgatcgccat ggcacaagcg tttctccaac
     1381 aagaggcggc ctgacttttt tgtcctctgg agcccgttga acagaactcc tgccggattc
     1441 ggcgaagcga acccttgacc aaccgaacac cgccaaaggt aaaattcggt catggcaagg
     1501 gcggctttct tattgccttc ttttttgtcc ccgtcgccct tgac
//
LOCUS       DATMTI010000519         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_613269, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000519 DATMTI010000000
VERSION     DATMTI010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613269"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VN039_08345"
     CDS             complement(<1..1114)
                     /locus_tag="VN039_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46013.1"
                     /translation="MSTRPPKARHYVIVVHGMGVQRESETAYEVIHRFAEARKGLDPD
                     SHITYRNLLPTSLSSQTVLAGKGHGWAEFRGIPVDPAEDTGPFDGLPATVSSGRNFRF
                     VDLRWSQILAEDEEDYACKLKVWTEALREKLKSVLPAPLQVQWADLLLQQIQNSALTV
                     QAALTYYRPAWATLIFEDILGDIHLYGDYARTRGKAVRHFHLVLDEIFVRDFLDWCRQ
                     EGDAPYQRPRLTVIAHSLGTIMSFDALMYAFANTAIRDGTGLHPCPSLPFPGYIEKRR
                     DEDEIWQRLASELKTRSLHQRLQQISGTTVLPEVPAIPALFWRDCVKEFITLGSPIDK
                     FHTLWYQNYLHMGFPNVSNIPTTWAEGWLQDVKTQIT"
     gene            complement(1752..3188)
                     /locus_tag="VN039_08350"
     CDS             complement(1752..3188)
                     /locus_tag="VN039_08350"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWV46014.1"
                     /translation="MAEMRGVPVKNSRQVMQIAVWGIVSGLMAPVSSPAGSLQIQTAN
                     GHDLQSQVKGTANKVIPAVVSIASTVMVRDQAFSDEALPFGLFKEPPTRRQYGQGSGV
                     IVTPDGYIITNNHVVADAVDVEVILADRRQYKGKVVATDPKTDVAVVKIHASNLPIAT
                     WGDSSHLAVGDFVLAIGNPLGLSRTVTFGIVSAVGRADVGVADFEDFIQTDAPINPGN
                     SGGALVNIHGELVGINTAIASPTGGSVGVGFAIPSNMARAAMQSLIKTGRVVRGFLGA
                     STQDVSPPLGKIFRLPDVKGAIVTDVQSKGSAERAGLKRGDVVVRFDGREVMDSGQLR
                     NLVAQSPIGSKHRIDLVRDGKQYQADLIIQEAPRERTKKSQMASSAVSTVHPLAGVVF
                     DDVTPPLARQMDLPVNNGVVVTDIEEGSLAETSGLQPGDVVLEMNRQHVSSFAVLQRF
                     ADPLKPTDLVLLLVNRQGNVMYIPIQGE"
BASE COUNT          724 a          934 c          849 g          749 t
ORIGIN      
        1 gtgtgatttg cgtcttcacg tcctgaagcc acccttcggc ccaggttgtc ggaatattgg
       61 agacgttggg aaatcccatg tgcaggtagt tttggtacca gagcgtgtga aatttatcga
      121 tgggtgaacc aagcgtaata aattccttca cgcaatcacg ccagaacaag gctggaatag
      181 cggggacctc aggcaacacg gtcgttccgc taatttgttg cagtcgttgg tgcaagctcc
      241 tggtttttaa ttcgctggca agcctctgcc agatctcgtc ctcatcccga cgcttctcaa
      301 tgtagccagg aaacggcaac gacggacagg gatgcaagcc ggttccgtct cgaatggcgg
      361 tattggcgaa cgcatacatc agagcatcga aggacatgat ggtgccaagg ctatgcgcaa
      421 tcacggtcaa tcgtggccgt tgatagggag catcgccctc ttgtcgacac caatcgagaa
      481 aatctcgcac gaagatttcg tccaaaacca agtggaaatg gcggaccgcc ttgccccgcg
      541 tccgtgcata gtcgccatac agatgaatgt cccccaggat gtcttcgaat atcagtgtcg
      601 cccaggctgg tcgatagtag gtcagtgcgg cttggaccgt gagcgcgctg ttctggattt
      661 gctgcaaaag gaggtccgcc cattgaacct ggagcggcgc aggtagcacg cttttcaact
      721 tttctcgaag ggcttccgtc cacactttca gtttgcaggc atagtcctcc tcgtcttctg
      781 caagaatctg cgaccaacgc aaatccacaa aacgaaaatt tcttccggaa cttactgttg
      841 cgggaagccc atcgaatggt ccggtatctt ccgcaggatc gacgggaatt cctcggaatt
      901 ccgcccagcc atggcctttt ccagccagaa ccgtctggga ggagaggctc gtgggcaaga
      961 gatttctata cgtgatatgg gaatcgggat cgagaccctt tcgggcctca gcaaatcggt
     1021 gaatcacctc ataggcggtt tcactttctc gctgtacccc cataccgtgg acgacaatca
     1081 cgtagtgtcg agcttttgga ggacgagtgc tcattggtct tcctcacgcc cagaagccaa
     1141 tggcgctatc gaacatgttg agatgacaca gttcatcggc aacagagccg cgacgtatgt
     1201 aaaagcattg agaacagtgt ccgaacgttc tccactcaag accgcgatct tcagcaagcg
     1261 caatccgtac cacaatacca accatcattg ccagcattca ttccgacgtt ggaactttat
     1321 cgatagcgtt ttcacgtatc caccggcagg gtacctcttt gagtccgggt actcgattag
     1381 atacaccttg tatctaaata gataaatcat agataaatct atgtaatggt tccatggctc
     1441 aatggatcat agaagactac ccgcaatcgt aggtaagccg atacaatgat gttcaatgtg
     1501 tgatcaaaaa tactgtcgcc cttgtcgctg tccttggttg aatgtcggta attgatatcc
     1561 ggacaggaga gccggccttt gaaagtcagc gccaaccgtg tcaatgcacc gtcgcgatgt
     1621 gagtgctgtg tgaccaggca cttgaatcag atcggtttat ttctcggcag gaagagtggg
     1681 aatcctcagg ctctatgcat ctctccattg cacgagcaag caaggcactt aagcgttgtc
     1741 atctcttcct cttattcccc ctgaatcggg atatacatca cgttgccctg ccggttcacg
     1801 agcagtagca cgagatcggt aggtttcagt gggtcggcga agcgttggag aacggcaaaa
     1861 ctgcttacat gctgtcggtt catttccagc acgacgtcac ccggctgcaa accggaggtt
     1921 tcggctaggc tgccttcttc aatatctgtc acgactacgc cgttgttcac cggcaaatcc
     1981 atctgccgcg ccagaggggg cgtgacgtca tcgaagacga cgcctgcaag cggatggacc
     2041 gtcgatacag ccgatgatgc catctggctt ttcttcgtgc gttcacgcgg cgcctcctga
     2101 atgatcagat cggcctggta ttgtttcccg tcccggacaa ggtctatgcg gtgtttactg
     2161 ccgatcggcg attgtgccac gagattccgc aactggccgc tgtccataac ctcccgcccg
     2221 tcgaaacgca ccacgacatc gcctcgtttc agacccgctc gttcggcaga gcccttggat
     2281 tgcacatcgg tgacgatcgc gcctttgaca tccggcaaac gaaaaatctt ccccagtgga
     2341 ggactgacgt cttgcgttga ggctcccaga aatcctcgca ccacacgacc cgtcttaata
     2401 agactttgca tggcggcacg ggccatgttg ctgggaatcg cgaacccgac acccacgctg
     2461 ccgccggtcg gactcgcgat ggccgtgttg atcccgacca gctcgccatg aatattgacg
     2521 agcgctccgc ctgaattccc cgggttgatc ggcgcatccg tctggatgaa gtcttcgaag
     2581 tccgccactc cgacatccgc ccgaccgacc gcgctcacga tgccgaacgt gacggtgcga
     2641 ctcaatccca gcgggttgcc gatcgcaagc acgaaatcac cgacggccag atgactcgaa
     2701 tctccccagg tcgcgattgg aagattcgac gcatggatct tgacgaccgc cacgtcggtt
     2761 ttcggatcgg tggcgacgac cttccccttg tattggcgac gatccgccag gatcacttcc
     2821 acgtcgacgg catcggcgac cacatggtta ttggtgatga tgtagccgtc aggcgtgaca
     2881 atcacacccg acccctgtcc gtattgccgg cgggtcggcg gctctttgaa cagaccgaac
     2941 ggtaaggctt cgtcgctgaa agcctgatcg cgaaccatca cggtggatgc tatactgacc
     3001 accgccggaa tgaccttatt ggcggtgcct ttgacttggc tctgcaaatc gtggccgttg
     3061 gccgtctgga tctgcaggct gccggccggc gacgaaaccg gagccatcag accgctcacg
     3121 atgccccaga cagcgatctg catcacttgc ctactattct tcaccggtac acctcgcatc
     3181 tcggccatat gtcaaacttc tcatacgcac ttttccttcc tcactgtggg acaaggaacg
     3241 acggcattct acactc
//
LOCUS       DATMTI010000520         4435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_401757, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000520 DATMTI010000000
VERSION     DATMTI010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401757"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..613
                     /locus_tag="VN039_08355"
     CDS             83..613
                     /locus_tag="VN039_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWV46015.1"
                     /translation="MSGAATDKEFKDLRRHAASHMDGGQFLTFSLGEELYGVDILRVQ
                     EIKGYTAVTKIPNTPPHIKGVLNLRGTIVPIVELRTKFGMPTIDYTTFTVIIVVVVQD
                     KVMGLVVDAVSDVLDIEKKDIQSAPDFGNKIDVTFLNGIGKSGDKLISLLDIDRLLLD
                     SAAKDSSKDLPVAAAA"
     gene            944..3454
                     /locus_tag="VN039_08360"
     CDS             944..3454
                     /locus_tag="VN039_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV46016.1"
                     /translation="MTILHVSALAAFCLMLAALCRWISRRPTEAAPPEERFHRLFEYA
                     AVAMSIVDSDKRFVHVNRAYSELLGYDTTELLGRPFTMVAHPEDIPEHLVLWDEVFRR
                     TECSSAQDKRYIRKDGRVIWVRVTANLFKPSDGTAPLVMAVVENITERKQAEQVLRQS
                     EARLIEAIPQQVWTARPDGTLDYVNRRVLEYFDVPAERLLDWGWQSVLHPDDLPACMT
                     AWTASLQTGEPYEIEFRLRQAGAGTYRWHLTRALPMRDETGRIVKWIGTNTDITGRRQ
                     VEEALRESEERFRVLADTAPVLIWMSGRDNRISYFNTRWLEYTGRTMEQELGDGWIEG
                     VHSDDVDRCWNIYSEAFDRREPFDLEYRLRKADGQYGWLHESGAPRFLPGGTFAGYHG
                     ACIDITARKLAEAELRRVHDELERRVSERTAQLAMANRSLQDEIAERKHVEEAVRDSE
                     LRYKLLTEATFDGIAIHDQGILIEVNAGLGRIFGYGPGELIGRSLWDLVADESRDLVL
                     ANMQNGVNGPYEAMGRRKDGTTFPGEVVVRPCRYRGKEVRLVAGRDITERKHLEAERS
                     RHTEELEHQIAERTAEIAKLESQRAQTEKLAAMGRLAAGVAHEINNPIAGIKNAFTLV
                     KQAVDPAHPHAEFVGMIDREITRVASIVQNMYQLYRPESGKGEAVELRSMMNDIEALF
                     SKRLQQRRLELAIDVDSCIERLRVPRGDLLQVLLNLLNNAIDCSSDGGTVTLAICVEP
                     DAIRIAVSDQGPGISPDNLPHIFDPFFTTKTERDQKGMGLGLSISQSLVMAMGGKIEV
                     RTQPNGGSTFSVLLPRHTAGACAQGQSNIIKEVVTHVD"
     gene            3444..4202
                     /locus_tag="VN039_08365"
     CDS             3444..4202
                     /locus_tag="VN039_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46017.1"
                     /translation="MLTETPRILLADDEETFRRSTAKLLEQEGYRCDCAQDSHEAGRL
                     LTRQHDALITDIRMPGNMQFEFLRDVRTRFPSLPIVLVTGYPSVQTAVEALRLSFTDY
                     LLKPVDWPDLLRAVGEAVKRTSLLRMTTAVREEASRLVASLEQLQDNLPRLGSGANER
                     ELAWSLDAFLSQSFAQMAVLSSRIRSVLTSQLQGGAEEPTDVCRMMQCPRLDAYREAL
                     ESTVEVLANTKHASRSQDLGLLRNKIEQLLREDA"
BASE COUNT          943 a         1204 c         1329 g          959 t
ORIGIN      
        1 gtagaagtct ggacagacgc cggcttgtcg gatcaagaag gggtgccgac gagacgggag
       61 acgatcggga aaggagcttt ccatgtcagg agcggctacg gataaggaat tcaaagatct
      121 tcgccgacat gcggcatcgc acatggacgg cggtcagttt ttgacattca gtcttggaga
      181 agagctgtat ggtgtcgata tcctccgtgt gcaggagatc aagggatata ccgccgtgac
      241 caagatcccc aatacaccgc ctcatatcaa aggagtgttg aatctgcgcg gaaccatcgt
      301 gcccatcgtc gaactacgga ccaagttcgg tatgccgacc atcgactaca cgacgtttac
      361 cgtcatcatt gtggtggtgg tgcaggacaa agtgatgggg ttggtggttg atgccgtatc
      421 ggacgtgctt gacatcgaaa aaaaggatat tcagtcggcg ccggattttg ggaataagat
      481 cgacgtgacg ttcttaaatg ggattggaaa gtccggcgac aagctgatat cgctgttgga
      541 catcgaccgg ttgttacttg attcagccgc gaaagattcc tcaaaggatt taccggtggc
      601 ggctgcagcg taaggagtcg gaccgagcgc cgcatccggc catgaagccg tcggccatgc
      661 cctctccctc tccctctctt ctttttcgtc tctaccggat tatccattag ttgatttaag
      721 caggggcttg cgtagggcca tatggatttg acggatgatg catggcgcgt tatcttgccg
      781 ccccgtgacc catcacggtt gtcggacgac tactgaagcc aacgtcggtc cccatcaagg
      841 gtatagcctt ccatcttcaa gcattctcgg tccttcgacc ataggccttg tggcgtttct
      901 tggcttcaac ggcttctacc tggctatctc agggtccgct tccatgacga ttctccacgt
      961 atcggccctt gcagcctttt gtctcatgtt ggcggccctg tgccggtgga tcagcaggag
     1021 gccaaccgaa gctgccccac ctgaagagcg cttccaccgt ctcttcgaat atgccgctgt
     1081 cgccatgtcg attgtcgatt ccgacaaacg gttcgttcat gtgaatcgtg cctactctga
     1141 gctgctcgga tacgacacga ccgagcttct cggcagaccc ttcaccatgg tggcgcatcc
     1201 ggaagatata ccggaacatc ttgtattgtg ggatgaggtg tttcgtcgca ccgagtgttc
     1261 gagtgctcaa gacaagcggt acatccgtaa agacggccgt gtcatctggg tccgagtgac
     1321 ggccaacctg tttaaaccat cggatggtac ggcgcctctc gtcatggccg tcgtcgaaaa
     1381 catcactgaa cgcaagcagg cagaacaggt tctgcggcaa agcgaagcac gattgatcga
     1441 agccattcct cagcaggtct ggacggcccg tcctgacggc acattagatt acgtgaaccg
     1501 gcgggtgctg gagtatttcg atgttccggc agagcgactg ctcgattggg gatggcagtc
     1561 tgtgctccat cctgacgacc tgcctgcctg tatgacggca tggaccgctt ctttgcaaac
     1621 cggagaaccc tatgagattg agtttcgcct caggcaggct ggcgccggca cctatcgctg
     1681 gcacctgacc cgcgccctgc cgatgagaga cgaaacaggc cggatcgtca agtggatcgg
     1741 gacgaacacc gacatcaccg ggcgccgaca ggtggaagag gccctgcggg agagcgagga
     1801 acggtttcgg gtcctcgccg acacggcgcc ggttctgatc tggatgtcgg gccgggacaa
     1861 tcgcatcagc tatttcaaca cccgctggtt ggaatacacc ggccgaacta tggaacaaga
     1921 gctgggagac ggttggatcg agggggtaca ttccgacgac gttgatcgct gttggaacat
     1981 ctattcggaa gcgttcgatc gacgtgagcc ctttgatttg gaatatcgtt tacgaaaggc
     2041 cgacgggcag tacggctggt tgcatgagag cggtgcgcct cgtttcctcc cgggaggaac
     2101 gtttgccggc taccatggcg cctgcattga catcacggcg cgaaagctgg cggaagcgga
     2161 gctacgacga gtgcatgatg aattagagcg tcgagtcagt gagcggaccg cgcagctggc
     2221 gatggccaat cgatctcttc aggacgagat cgccgagcgg aaacatgtgg aggaggcggt
     2281 acgcgacagc gaattgcggt acaagctgct gacggaggcg acgttcgacg gcatcgccat
     2341 ccacgatcag gggatcctga tcgaggtgaa tgcgggtttg gggcggatat tcgggtacgg
     2401 accgggcgag ctcatcggcc gatccttgtg ggatctcgtc gccgacgaat cccgcgatct
     2461 ggtcctggcc aatatgcaga acggcgtgaa cgggccctac gaagccatgg ggcgtcgcaa
     2521 agacggcacc acttttcccg gtgaagtcgt agtccggccc tgtcgttatc gtgggaaaga
     2581 agttcgactt gtggcgggcc gtgacattac cgagcgcaag cacttggagg cggagcggtc
     2641 ccgtcatacg gaggagttgg agcatcagat cgccgaacgg accgccgaga tcgccaagtt
     2701 ggaatcacag cgtgcgcaga cagaaaaact ggcggcgatg ggccgcttag ccgccggcgt
     2761 cgcgcatgaa atcaataatc cgatcgccgg gatcaagaac gcctttacgc tcgtgaagca
     2821 agcggtagat cccgcccatc ctcacgccga attcgtcggc atgatcgatc gtgagatcac
     2881 tcgcgtcgca tccatcgtcc agaacatgta tcaactctat cggccggagt ccggcaaggg
     2941 ggaggcggtg gaactccgat cgatgatgaa cgacatcgag gcgcttttct ccaaacggct
     3001 gcaacagcgc cggctcgagc tggcgatcga tgtggattcc tgtatcgagc ggctccgcgt
     3061 accgcgaggg gatttgctgc aagtcctgtt gaatctgctg aataatgcca tcgattgttc
     3121 cagcgacgga ggtacggtca cactggccat ttgtgtggaa ccggatgcga tacgaattgc
     3181 cgtgtccgat caagggccag gcatttcccc ggacaatctg ccgcacatct tcgacccatt
     3241 ctttacgaca aagacggaga gagaccagaa gggcatgggg ctggggcttt ccatctctca
     3301 gagcctggtg atggcaatgg gcggcaagat cgaggtccgg actcagccga acggcgggtc
     3361 gacgttttca gtcctcttac cgcgtcacac ggctggtgca tgtgcgcaag gtcagtcgaa
     3421 tatcataaag gaggttgtca ctcatgttga ctgagacacc gcggattcta ctcgccgacg
     3481 atgaagaaac gttccgtcgc tcgacggcga agcttttgga gcaagaggga tatcgctgtg
     3541 attgcgcgca ggactctcat gaagccggta ggctcctgac gagacaacat gatgccctca
     3601 tcaccgatat tcgcatgccg ggcaatatgc aattcgaatt tctccgggat gtccgtaccc
     3661 ggttcccttc gcttccgatc gtgcttgtga ccggctatcc ctcggttcaa actgccgtgg
     3721 aagcactccg cctgtcgttt accgactacc tgctcaagcc ggtggactgg ccggatttgc
     3781 ttcgtgcggt gggcgaagcg gtgaagagga ccagtctttt gcgcatgacc acagcggttc
     3841 gagaagaagc gagtcgcctg gttgcttcct tggagcagtt acaagataac ttgccccgac
     3901 tgggtagcgg ggctaacgaa cgggaactgg catggtcgct ggatgcgttt ctttcccaga
     3961 gtttcgcaca gatggccgtc ttgtcttcca gaatccgatc cgttctgacg agtcaattgc
     4021 aaggcggcgc cgaggagcct acggatgttt gccgcatgat gcaatgtccg agactcgatg
     4081 cgtatcggga agcacttgaa agtacggttg aggtgctggc aaacaccaag cacgcatctc
     4141 gatctcagga cctgggactg ttacggaata aaatcgaaca gttactccgc gaggatgcat
     4201 gaattcataa tcgccgccaa gtccatccac cacagggccg acatcgtggc tggtccgggg
     4261 ctcgctcgcc aagggtcgat cctcacgaat gtagggatcg catcggcggt gttctttgcc
     4321 gaaccgatgg atcctcccga gcaacccttc ccgaagcact ctttcccatg gcctctccgt
     4381 ttttctgact gtgggtgtgt cttccgccgc cgcttaaggt ttcccattcc gaacc
//
LOCUS       DATMTI010000521         1960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_571062, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000521 DATMTI010000000
VERSION     DATMTI010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571062"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1316
                     /locus_tag="VN039_08370"
     CDS             354..1316
                     /locus_tag="VN039_08370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWV46018.1"
                     /translation="MNSALQAFGRHSISVVTPVYNGEASVAELCRRLGELLPRIATEY
                     EIILVNDGSRDGSWDVISELSSRFPTVRGLNLMRNYGQHNALLCGIRAAKYDVIVTMD
                     DDLQHPPEEIPRLLEQMEQGFDVVYGAPKEERHVFMRALASRITRLALGSAIGMDLAK
                     NVSAFRVFRTQLREAFADYQTPFVSIDVLLTWATTRFTAVPVAFQPRYSGTSTYTFTK
                     LVRHALDMMTGFSTAPLQLASLIGFACTLFGLAVFLYVFVRYCFEGSVPGFPFLASIV
                     AIFSGAQLFALGVIGEYLARIHFRTMNRPVYVVRSVSGESKDLP"
     gene            1359..>1960
                     /locus_tag="VN039_08375"
     CDS             1359..>1960
                     /locus_tag="VN039_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46019.1"
                     /translation="MKEALPRFTHRPLPHLTYLKPDQIDGFWLDDIAQDLNNGNSIAF
                     ASYHDDRINGLVLYGDSPWDAKVVGKSVAVIKYFVGSGEDDPRMLNQLLDEVLQRAGS
                     RGICLVTCKVQSLQFAAIHALERHGFLLMDTLVDFVFDFSHTSFDKITPPQRLSGLNV
                     RLANPEDLPELLALTEIAFAQHFGRYNSDPEMPAGTGSRV"
BASE COUNT          423 a          531 c          509 g          497 t
ORIGIN      
        1 gcagtgtaag ggctacttct gatcctgcgc gcgctgccga atttcgcttg tcatgttgag
       61 cgaagcgctg caacgaaatg cgaagcatga aggccaggct ttcaaacctc tttgatttct
      121 ttcctcccag agcaaatgca aatccagaga ttgaaagcct gggcgcgccc cgaaaggttt
      181 tcggggctgc atcgaagctt cgcttcgctc agaatgacac gttatcgaaa cggcgccggt
      241 catcaccgat tgtccactct catctgctac agctaacgga cgcaaggtta aattgcgtgt
      301 cagtgccgct ttgacgaaat cggcctcgtc tcatagcctg aggcttttct gacatgaact
      361 ccgcgttgca agctttcggg cggcattcca tttcggtcgt gacgccagtt tacaatggcg
      421 aggcttctgt tgcggaactc tgcaggcgtc ttggggaatt gttgccgcgg atcgcgacag
      481 agtacgagat catcctcgtc aacgacggca gtcgggacgg gagttgggac gtgatctcgg
      541 aactctcgtc acgctttcca actgtgcgcg gtttgaattt gatgcgcaat tatggccagc
      601 ataacgcgct gctctgcggg attcgcgctg caaaatacga cgtcatcgta acgatggacg
      661 acgatctgca gcatccgccc gaggaaatcc cgagactact cgaacaaatg gaacaaggat
      721 ttgatgttgt ttatggcgcg cccaaggaag aacggcatgt gtttatgcgc gcccttgctt
      781 cccgcattac caggctcgcc ctcggtagcg ccatcggaat ggatcttgca aaaaatgtaa
      841 gtgcattccg tgttttccgt acgcaattgc gcgaggcatt cgctgattat cagacaccat
      901 ttgtttccat cgacgtgtta ctgacatggg cgacaacacg gttcaccgca gtacccgtcg
      961 ccttccagcc ccgctactcg ggaacttcga cttacacttt cacaaaactg gttcgacacg
     1021 cgctcgatat gatgaccggg ttcagcacgg ctcccttgca gctcgccagc ctgattggat
     1081 ttgcctgcac gttattcggg ctcgcagttt tcctctacgt cttcgttcgc tattgctttg
     1141 aaggcagtgt gcctggattt ccatttctgg cttccatcgt cgcgatcttt tcgggcgcac
     1201 agctcttcgc tcttggcgtg ataggcgaat acctggcgcg cattcatttt cggacgatga
     1261 atcgtccggt ttacgttgtt cggagcgtta gcggcgaatc aaaagacttg ccataatccc
     1321 ggatgatcgc gccattcgat ctgacggcca ttgagagctt gaaagaggcg ctgccacgct
     1381 tcacgcatag acctctgcca cacttaacgt atttgaagcc agaccagatc gacgggtttt
     1441 ggctggacga catcgctcag gatttgaaca acggaaattc gatcgcattt gcatcatatc
     1501 acgatgaccg catcaacggt cttgtgcttt acggcgattc gccgtgggac gcgaaagtcg
     1561 tcggtaaatc agtcgccgtc ataaaatatt tcgtcggctc cggtgaggac gatccacgta
     1621 tgctcaatca attgctggat gaagtgttac agcgtgccgg cagtcgtgga atctgtcttg
     1681 tgacatgcaa agttcagtcc cttcaatttg ccgcgattca cgcgctggag cgacatggat
     1741 tcctattgat ggacacgctg gtcgattttg tctttgactt ctctcacacc tctttcgaca
     1801 aaatcactcc accccaacgg ctaagcggat tgaatgttcg gttggcgaat ccggaagacc
     1861 tgcctgaact gcttgcgctg actgaaatag cttttgctca acatttcggc agatacaact
     1921 cggatcccga aatgcccgcc ggcaccggca gcagggtata
//
LOCUS       DATMTI010000522         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_613313, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000522 DATMTI010000000
VERSION     DATMTI010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613313"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1827
                     /locus_tag="VN039_08380"
     rRNA            297..1827
                     /locus_tag="VN039_08380"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          405 a          470 c          699 g          471 t
ORIGIN      
        1 actgcactga gagctaaggc caggccggtg gggagaccca ctcgggtggc tggtaagctc
       61 gatgagtcgc tcggttccag ggcccgctaa gggtcgatga gccgggtaag ttagagtgag
      121 tcatcccgga tccaactgcc gcttcggtgg ttcgtgatgg tgtgtgtttg ttctttgaga
      181 actcaacagt gtgctgaatt tttgttagtg ccatgtttgt ttgtgttgtt gttgtgctag
      241 tgatcagtgc gatgtctgcc ctgtagtggg tgggtgttgt tttctgttga tgacattcaa
      301 tggagagttt gatcctggct caggacgaac gctggcggcg tgcttaacac atgcaagtcg
      361 agcggtaagg cccttcgggg tacacgagcg gcgaacgggt gagtaacacg tgggtgatct
      421 gccctgcact tcgggataag cttgggaaac tgggtctaat accggatagg accatggcat
      481 gcatgtgctg tggtggaaag cttttgcggt gtgggatggg cccgcggcct atcagcttgt
      541 tggtggggtg atggcctacc aaggcgacga cgggtagccg gcctgagagg gtgtccggcc
      601 acactgggac tgagatacgg cccagactcc tacgggaggc agcagtgggg aatattgcac
      661 aatgggcgca agcctgatgc agcgacgccg cgtgggggat gacggccttc gggttgtaaa
      721 cctctttcac catcggcgaa gccggatgct tttgtgtctg gtgacggtag gtggagaaga
      781 agcaccggcc aactacgtgc cagcagccgc ggtaatacgt agggtgcgag cgttgtccgg
      841 aattactggg cgtaaagagc tcgtaggtgg tttgtcgcat tgtccgtgaa attccctggc
      901 ttaactgggg gcgtgcgggc gatacgggca gactggagta ctgcagggga gactggaatt
      961 cctggtgtag cggtggaatg cgcagatatc aggaggaaca ccggtggcga aggcgggtct
     1021 ctgggcagta actgacgctg aggagcgaaa gcgtggggag cgaacaggat tagataccct
     1081 ggtagtccac gccgtaaacg gtgggtacta ggtgtgggtt tccttccttg ggatccgtgc
     1141 cgtagctaac gcattaagta ccccgcctgg ggagtacggc cgcaaggcta aaactcaaag
     1201 aaattgacgg gggcccgcac aagcggcgga gcatgtggat taattcgatg caacgcgaag
     1261 aaccttacct gggtttgaca tgcacaggac gccggtagag atatcggttc ccttgtggtc
     1321 tgtgtgcagg tggtgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc
     1381 gcaacgagcg caacccttgt ctcatgttgc cagcacgtta tggtggggac tcgtgagaga
     1441 ctgccggggt caactcggag gaaggtgggg atgacgtcaa gtcatcatgc cccttatgtc
     1501 cagggcttca cacatgctac aatggccggt acaaagggct gcgatgccgt gaggcttagc
     1561 gaatccttta aagccggtct cagttcggat cggggtctgc aactcgaccc cgtgaagtcg
     1621 gagtcgctag taatcgcaga tcagcaacgc tgcggtgaat acgttcccgg gccttgtaca
     1681 caccgcccgt cacgtcatga aagtcggtaa cacccgaagc cagtggccta acccttgtgg
     1741 ggggagctgt cgaaggtggg atcggcgatt gggacgaagt cgtaacaagg tagccgtacc
     1801 ggaaggtgcg gctggatcac ctcctttcta aggagcactt ggcagccggc gcaagcctgg
     1861 ttgtccattg agctgtttgt acggcgaatg tctgtacgca gctcagctca ctagtggaac
     1921 acaacgatct ttgctcatct gtgacagcca ttgtttggtc tgttgggggt gggggtgtgt
     1981 ggcacgctgt tgggtgtctg aggcatcaga cgatccctgc ctggtagctc ttgttgcttc
     2041 gtcgg
//
LOCUS       DATMTI010000523         3928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_19892, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000523 DATMTI010000000
VERSION     DATMTI010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19892"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VN039_08385"
     CDS             <1..1146
                     /locus_tag="VN039_08385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003333.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HWV46020.1"
                     /translation="NLLAFQSHEERNSTMATKQKQATAVEKKLIGLADIVVQAAERSK
                     DPTFQIPIRALSNVSFNERKGLIEMGSKKQERSFFNVGMAKKFMQTVLVADALSELQR
                     ADLTTSLREIYYRTKHTIKDSHENTFDTQDESDPVIEDLEVSLAALREELHVRAENSG
                     SIVGPVVFGDDGDRVDCSKLGKGGYSVPSIVEPEYLEIRRCTADFVLLVEKGTQWNRL
                     SEDKFWRRYNCVLLTGNGQPPRGVRRLARRLHEEHRLPVYVLVDNDPWGYYIYSVVKQ
                     GSINLAFESQRMAIPKAKFMGLSSADPEQYELPRNVGIKLNEKDIARAKELMNYQWFQ
                     KPAWQAEIKRMLTSGLKYELDALANKDFQYLTKKYLPKKLQERDWLD"
     gene            1179..2738
                     /locus_tag="VN039_08390"
     CDS             1179..2738
                     /locus_tag="VN039_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWV46021.1"
                     /translation="MKKLPAWTAPLRDWQRRALSVVCTHQTKDFLAMATPAAGKTRFA
                     LRVAHEFMAKQAAIRVLVVCPTNHLRTQWSDAAGKIGLQLDPTLTNEQAAEAPDYHGA
                     VVTYQQVCLAPASFQRVCKSKKTLLIFDELHHAGDGKNWGKALRSAFDPAVFRLILSG
                     TPFRSDNNPIPFIRYEHGESRADFAYGYTDAIRDSVCRPIVFPSYEGELSWLSDGREH
                     QATFEDGLTFNRQRERLKTALLQETWLGPVITDAHAQLSRLRKQEQPDAGGLIVCMEQ
                     EHARWVADLVGRIVGTKAAVAVSDDPMASRTIEEFADHKKQQWLVAVNMVSEGVDIPR
                     LRVGVYGTNVITEMYFRQVVGRFVRMQEGLPKPQRAWLYLPKDPVLVHYAKQIKAERD
                     HVLEDIMPAGQRDLFGHVTVSTNEYMPLMAVARMDSLIGEDEMRGEIDRNAVTEPAVS
                     LHEQKQDLRDLHRLLVAGVARNSGIDHRRLNAELIARTGSRVDQATTEQLRKRIQLLE
                     RWKERGYDGKR"
     gene            3184..3759
                     /locus_tag="VN039_08395"
     CDS             3184..3759
                     /locus_tag="VN039_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46022.1"
                     /translation="MTGVSMLGCTTVVTPLQPGYLPSMMKSSHGLVLGRMHLVWNGRE
                     QRTGARFPLHVRLRITEEESGAQVVIDRVPVDGPFVLDLPAASYRLTVVSLHNALGVW
                     QTSLPTTFSVWPQECTYLGTWELQMQTEFFSGAMTRQVFDQRDLAARDLHAIIGHDQA
                     PPPVVAQLATLMQSPLVLMFQTEGTQLTSPP"
BASE COUNT          890 a         1046 c         1184 g          808 t
ORIGIN      
        1 aacctactcg cgtttcaaag ccacgaggaa agaaatagca ccatggcgac gaaacagaaa
       61 caagcgactg ccgtcgaaaa gaagctgatc ggcctagcgg acattgtggt gcaagcggct
      121 gagcgatcga aagacccgac atttcagatc cccatccgtg ccctctcgaa tgtgtccttc
      181 aatgaacgga agggcctgat tgagatgggg agcaagaagc aggagcggtc gtttttcaac
      241 gtcggcatgg cgaagaaatt catgcaaaca gtgctggtcg ccgacgccct ctccgaattg
      301 cagcgggctg atctgaccac gtcgcttcga gaaatttact atcgtacaaa acatacgatc
      361 aaggactcgc acgagaatac gttcgatacg caggatgaat cggatccggt gatcgaagat
      421 ctggaagtct ccctcgccgc gcttcgggag gagctccatg tacgtgcgga gaacagcggc
      481 agcatcgtcg ggccggtcgt cttcggcgat gacggcgatc gtgttgattg ttcaaagctc
      541 gggaaaggcg gctactcggt gccgtcgatt gtggagccgg agtatttgga gatccggcgc
      601 tgcaccgccg atttcgtgtt gctcgtggaa aagggcacgc agtggaaccg gctgtcggag
      661 gacaagttct ggcgtcgcta taactgtgtg ttgcttacag ggaacggtca gcctccacgg
      721 ggtgtgcggc gcttggcgag acggctccat gaggagcatc ggttgccggt ctatgtcttg
      781 gtcgacaatg atccgtgggg atactacatc tattccgtcg tcaaacaggg ctcgatcaat
      841 ctggctttcg agagccagcg catggccatt cccaaagcca agttcatggg cttatcgagt
      901 gccgacccgg agcagtacga gctgccgcgc aatgtcggca tcaagctcaa cgagaaagac
      961 atcgcccgcg cgaaggaact gatgaactac cagtggttcc aaaagcccgc ctggcaggcg
     1021 gagatcaagc gtatgctgac gagcggattg aaatatgaac tcgacgcatt ggccaataaa
     1081 gacttccagt atctcaccaa gaaatacctg ccgaaaaaac ttcaggaacg ggattggctc
     1141 gactagcctc ctccgcgaac cggcacgacg gaccggccat gaagaaattg cctgcctgga
     1201 ccgcgcccct tcgtgactgg cagcgacgag ctctgtcggt ggtctgtacc catcagacca
     1261 aagactttct ggcaatggcc actcctgccg cgggaaagac gcgctttgcg ctgcgcgtcg
     1321 cgcacgagtt tatggcgaaa caggctgcga tccgggtgct ggtcgtctgt ccgacgaatc
     1381 atcttcgcac gcaatggtcg gatgccgcgg ggaagatcgg tttgcagctt gacccgaccc
     1441 tgaccaacga gcaggcggct gaagcgccgg actatcacgg cgctgtggtc acctaccaac
     1501 aggtctgtct ggctccggcg agcttccagc gggtctgcaa gagcaagaag acgttgctca
     1561 tcttcgacga gttgcaccac gcgggcgacg gcaagaactg ggggaaggcg ctgcgctcgg
     1621 cgttcgaccc ggcggtgttt cgattgattc tctccggtac gccgttccgg tcggacaaca
     1681 atccaatccc gtttattcga tatgaacatg gagaaagccg ggcggatttc gcctacggct
     1741 ataccgacgc gatccgcgac agcgtctgcc ggccgatcgt ctttccgagc tatgaaggtg
     1801 agttgagttg gttgtccgac ggccgcgagc atcaggcgac atttgaagac ggattgacgt
     1861 ttaatcggca gcgcgaacgg ctgaagaccg cgttgcttca ggaaacctgg cttgggccgg
     1921 taatcaccga tgcccatgcc caattgagcc gccttcggaa acaggaacaa ccggatgccg
     1981 gtgggttgat cgtgtgtatg gagcaggaac acgcacggtg ggtggcggat ctcgtcggtc
     2041 gtattgtcgg caccaaagca gccgttgcgg tgtcggacga tcccatggct tcccgcacga
     2101 ttgaggagtt tgccgatcac aaaaagcagc agtggctggt ggcggtcaac atggtgagtg
     2161 aaggcgtgga tattccgcgg ctccgtgtcg gcgtctatgg aacgaacgtg atcacggaaa
     2221 tgtacttccg acaagttgtc ggccgatttg ttcgaatgca ggaggggctg cctaagccgc
     2281 aacgggcatg gctctatctg ccgaaagatc cggtcttggt gcattatgcc aaacagatca
     2341 aggcagagcg cgatcatgtg ttggaagaca tcatgccggc aggccaacgg gatctcttcg
     2401 gtcacgtgac ggtctcgacg aacgagtaca tgccgttaat ggccgtggcc aggatggata
     2461 gcctgatcgg cgaagacgag atgcgagggg agatcgacag gaatgccgtt acagaaccgg
     2521 cggtctcact ccatgaacag aagcaagacc tgcgagatct tcaccggttg ctggttgccg
     2581 gcgtggcacg aaacagcgga atcgatcatc gccggctcaa cgcggaattg atcgcgcgta
     2641 ccgggagccg tgtcgatcag gcaaccactg agcagctgcg gaagcgcatt caactgctgg
     2701 agcgttggaa agagcgaggg tatgacggca aacggtgatc accacctgca caaggacccg
     2761 tgataaatag tcccgcagtc ttcactcatt gaaacgacga ctgctatggc gtattgttcc
     2821 cgaacacaag tggccggtta tacgggtagg aaatcgaaga tccggcgctt gtgcgggtcg
     2881 cctacgccct ctggtctggc gcaggtattg tcttaattgc gttgattgct tgggctctgt
     2941 acggccaaac tctcgaccta ccgggaatca taggtatgtc actcattgtc gccggcattg
     3001 ttattctcaa tctctcgtcc aataccgtct ctcactcatg agctggctca gctcgaagca
     3061 gaagagggtg tcatgagtat aaggggcgct ttcaaagagt ggctaccagc gtatgcgaga
     3121 ggagccgtaa aacgtgcaga cagcaaccgg cggggaaggt tccgctgtgg accactcctc
     3181 cttttgaccg gggtatcgat gcttggatgt accaccgtcg tcactccctt gcaaccaggc
     3241 tatctgcctt ccatgatgaa gtcgagtcat ggattggtgc ttggacggat gcatctcgtg
     3301 tggaacggaa gggagcaacg gacaggtgcg cggtttcccc tccatgtcag gttgcggatt
     3361 acggaggagg aatcaggggc tcaggtcgtg attgatcgtg ttccggtcga tggtcctttc
     3421 gtattggatt tgccggctgc ttcgtatcgc ctgacggttg tcagcctcca taacgcgttg
     3481 ggcgtctggc agacatccct cccgaccacg ttcagtgtct ggccccagga atgtacttat
     3541 ttggggacat gggagttaca gatgcagact gagtttttct caggggcaat gacgcggcag
     3601 gtgttcgacc agcgggatct cgccgcgcgg gatctccatg ccatcattgg acacgaccaa
     3661 gcgccgcccc cggtggtggc acagttggcg acactcatgc aaagtccgct cgtcttgatg
     3721 tttcagaccg agggcacgca gctgacatca ccaccatgac caaactcgag tccattcggg
     3781 aataattctc agcaagatat ttcaattcgt caaaaagcat aaaatgggcc tgttccaagg
     3841 gttgagaact gcccttcgat cccgacaaaa acgacacgcc caagaaagaa aggcaatccc
     3901 cagtcgaagg tagaagacgg tccgccca
//
LOCUS       DATMTI010000524         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_571263, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000524 DATMTI010000000
VERSION     DATMTI010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571263"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1301)
                     /locus_tag="VN039_08400"
     CDS             complement(111..1301)
                     /locus_tag="VN039_08400"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HWV46023.1"
                     /translation="MAYSSFKKALEAKLADIKSQGLYKAERRLLSPQGSDIRVAQGSV
                     LNLCANNYLGLANHPAIVQAATKGLETHGYGMASVRFICGTQDLHKQLEQAISEFLGT
                     EDSILYSSCFDANGGLFEVLLDEGDAVISDTLNHASLIDGIRLCKAKRFRYAHSDMAD
                     LEAQLQAAGGCRLRLIATDGVFSMDGDPAKLDRIVELAERYDATVVVDDSHATGVLGP
                     QGRGTAAHFGVADRIEMVTSTLGKTLGGATGGFTSGKTEVIELLRQRSRPYLFSNSLP
                     PPIAAGALRALDLVRQGDHLREQLRGNAAFFRAELTKLGFRLIPGEHPIIPVMLGDAA
                     LATSMAEALLKEGVYVVGFSYPVVPQGQARIRTQMSAAHTTTQLQQAVAAFAKVGRAL
                     GVIQ"
     gene            complement(1401..2435)
                     /gene="tdh"
                     /locus_tag="VN039_08405"
     CDS             complement(1401..2435)
                     /gene="tdh"
                     /locus_tag="VN039_08405"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HWV46024.1"
                     /translation="MQALVKTTAGSGLTLTDWVDPIPGPHDAVVKVAATSLCGTDVHI
                     YRWDEWAQRRIHPPRIIGHELCGHVVEIGREVSLVKVGDYVAAESHLTCGACFQCRTG
                     QAHVCKNYKILGIDRDGSYAEYVVLPEGVLWHTAPEIPPELACVQEPLGNAVDAALAE
                     DLTGHTVLITGCGPTGLFAAAVARTAGAATIIASDVSDYRLGLAKQVGADHVLNVRAE
                     SPDRVAAAILAMTAGEGVDAALEMSGDPTAMHQAFRAVKNGGRVTLFGIPTGPVCFDL
                     PNEVIFKGIRVYGITGRRLFGTWYRLAGLFKAGLDIRPVVTHSFPLGEFATGFELIRS
                     GQCGKVVLFP"
     gene            2679..>3130
                     /locus_tag="VN039_08410"
     CDS             2679..>3130
                     /locus_tag="VN039_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWV46025.1"
                     /translation="MKTRTNWMQAIVIASLLGYFPLGVEAETARWDIDPDHSIIEFRV
                     AHMVVSKTSGRFLNYRGFVDLDADTKAWKTIEATINAESINTNHEKRDTHLRSADFLD
                     VKQFPTITYKMKNYQKEGDTYKVVGNLALRGVTNEVTLIGTFNGITKDP"
BASE COUNT          710 a          931 c          859 g          630 t
ORIGIN      
        1 agcgtttctt gaagaatgac tttcctaccc ctactccttc acataataaa gtgcccgagg
       61 atactgagcc ggctgcgaaa tgtcaattct gaggggtagc ttgaggccaa ttattggatg
      121 acaccgagtg cccggcccac cttcgcgaaa gccgccacgg cttgctgcag ctgagtcgtg
      181 gtatgggctg ccgacatttg agtgcggatc cttgcctgcc cttgcggcac caccggatag
      241 ctgaatccga cgacgtagac tccttccttc agtaacgcct ccgccatgga ggtcgcaagg
      301 gcggcatcac ccaacatcac gggaatgatg ggatgttcgc ccgggataag ccggaagccg
      361 agcttggtca gctcagcccg gaagaaggcg gcattgcccc gaagctgctc tctgagatga
      421 tcgccttgtc tcacgagatc gagcgcgcgc aatgctccgg ccgcgatggg cggaggaagc
      481 gagttggaga agaggtaagg ccgcgatcgt tgacgcagca gctcgatcac ctcggtcttg
      541 ccggatgtaa acccgccggt cgcgccacca agcgttttcc ctaacgtgct cgttaccatc
      601 tcgattcggt cagccacacc gaaatgagcc gccgtccccc tgccctgagg acccagcacc
      661 cctgtcgcgt ggctgtcatc gaccacgacc gtcgcgtcgt accgttccgc caactccacg
      721 atccgatcca gtttggccgg atcgccatcc atcgagaaca ccccatcggt tgcaatcaga
      781 cgtagccggc acccaccggc tgcctggagc tgcgcctcga ggtccgccat gtcggaatgg
      841 gcgtatcgga atcgcttggc cttacagagc ctgatgccgt cgatcaagct ggcatggttc
      901 aaggtatcgc tgatgacggc atcaccctca tccaacagta cttcgaatag tcccccgttg
      961 gcatcgaagc aggagctgta caagattgag tcctcggtgc cgagaaactc actgatggcc
     1021 tgctccagtt gcttgtgcaa atcctgcgtg ccgcaaataa accgcacgga agccatgcca
     1081 tatccatgcg tttccaatcc ctttgtggcg gcctgcacga tggccggatg attcgcaagc
     1141 cctagataat tgtttgcaca gaggttcaga accgagcctt gcgcgactcg gatgtcggag
     1201 ccttgcgggc tcagcaatcg gcgttcggcc ttgtacagac cttgggattt gatatcggca
     1261 agcttagctt caagagcttt cttgaaggac gaataggcca tggttagaac aggactgaga
     1321 gatgaggact gagggccgag gactgaatga agggcaagcc ttctgttctc tcagtcctca
     1381 gtcctcgtac ctcagtccta ctacggaaac agcaccactt tgccgcactg ccctgatcgg
     1441 atcaactcaa acccggtggc aaattcgccc agcggaaagg aatgggtcac gaccggccga
     1501 atatcgagac ctgccttgaa gagaccggcc agccgatacc aggttccgaa caagcgccgc
     1561 ccggtgatcc catagacacg gatgccctta aaaataactt cgttcggcag atcaaagcaa
     1621 acgggaccgg tcgggatgcc gaatagggtt actcgtccgc cgttcttcac cgcgcgaaag
     1681 gcttgatgca tagccgtggg atcgcccgac atctccagtg ctgcatcgac cccttcacca
     1741 gcggtcatcg cgagaatggc cgccgccact cgatccggtg actcggctct cacgttgagg
     1801 acgtgatctg cgccaacctg cttcgccaag ccaagccgat agtcactgac atcggatgcg
     1861 atgatggtcg cagcgccggc ggtacgtgcg acagccgcgg caaataaacc ggtcgggccg
     1921 caccccgtga tcaacacagt atggccggtg agatcttcag cgagagcagc atcaacggca
     1981 ttaccaagcg gctcctgcac acaggccaac tccggaggaa tttccggagc cgtgtgccac
     2041 aacactccct ctggcaacac cacgtattca gcgtaagagc cgtctcgatc gatcccaaga
     2101 atcttgtagt ttttgcacac atgcgcctgt ccggtgcgac actgaaagca cgctccacag
     2161 gtcaagtgtg attcggcggc gacatagtcg ccgactttca ccagggatac ctcccgccca
     2221 atctccacga cgtggccgca caattcatgg ccgataattc gtggtggatg aattcgccgt
     2281 tgcgcccatt cgtcccaacg atagatatgg acgtcggttc cacataacga ggtcgcagcc
     2341 accttcacca cagcgtcatg tggccccggg attgggtcca cccaatcggt gagggttaaa
     2401 cccgaccctg ccgtggtttt cacgagtgct tgcatgaacc tattctatac caactcggtt
     2461 atttgacacc cattttcggt ggagtcccca cggctataca gtcatggtct cccatggttc
     2521 ttcggtgaca cctcgccgaa gtgcggcttc ctacgtaggc gggtgaaaga aacgggttgc
     2581 atgggattcc cagcgatgag taggatgccg ctcaagccgt ggcatcgccc aagaggtagg
     2641 tgtccgttca cacaagccga caggggaaag gtgatgatat gaaaacccgg acaaattgga
     2701 tgcaggcaat cgtcatagcg agtctattgg gatacttccc gctgggcgtc gaggcggaaa
     2761 cggctcgttg ggacatcgat cctgatcatt cgatcatcga gtttcgcgtg gcccacatgg
     2821 tggtgtcaaa aacgtcgggg cgtttcctga actatcgtgg atttgtcgat ctggacgcgg
     2881 acacgaaagc ctggaagacg attgaagcca cgatcaatgc tgaatcgatc aacacgaacc
     2941 acgagaaacg cgacacgcat ctgcgcagtg ccgacttctt ggacgtgaaa cagtttccga
     3001 cgatcaccta caaaatgaaa aactaccaga aagagggaga tacctataag gttgtcggca
     3061 acctggccct acgcggcgtg accaacgagg tcacgctcat cggcaccttc aatggcatca
     3121 ccaaggatcc
//
LOCUS       DATMTI010000525         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_190134, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000525 DATMTI010000000
VERSION     DATMTI010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190134"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VN039_08415"
     CDS             complement(<1..365)
                     /locus_tag="VN039_08415"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46026.1"
                     /translation="MGLDKLQITPERGQPIHALFNPERYTVNKAVQFAEIAIPGLDSP
                     VVQFVRGQAEKITMDLFFDTTQYGMVDDVKDVRELTGQVYQLTKVHSETHAPPRCVLL
                     WGKAGKLFSFGTKVSPWCVV"
     gene            complement(376..570)
                     /locus_tag="VN039_08420"
     CDS             complement(376..570)
                     /locus_tag="VN039_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46027.1"
                     /translation="MDGPDIRIGEVVTDIVVTEGVGTLSADDVKRIVALVVEHLRHEQ
                     ERITQRTRDTAIHNQAYQRS"
     gene            complement(572..1060)
                     /locus_tag="VN039_08425"
     CDS             complement(572..1060)
                     /locus_tag="VN039_08425"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWV46028.1"
                     /translation="MASGFRIDPLGVFNFYLTLIDSSNVVGTLITAALNYKVAGFSEC
                     SGLDATVEVFEYKEGGQNGFVHKFPTRASHANITLKHGVIYLYDDLWTWHYDFVQGKG
                     TRKDGLIVLLNEARKPVKVWKFKKGIPMKWVGPSLNAGQSNVAVESLEISHQGLLLEV
                     GA"
     gene            complement(1073..2104)
                     /locus_tag="VN039_08430"
     CDS             complement(1073..2104)
                     /locus_tag="VN039_08430"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HWV46029.1"
                     /translation="MTPSDLIGGTDDLRGLRLLEEIDEVSILCLPDAVPCFASRATIE
                     PPRPSSDPCDPGATPSTPLAKELPVDKTSRSPSWTSDQTTLIYRAMIDQCERLRDRVA
                     ILDYLPTRAVPTAAQLLAWRNQFITRFGAIYYPWFLVPDAANPAGPPRPLPPSGHVAG
                     IYARIDNQFGVQRPPGNEPFEFVTDVQQEITSLQQEQLNPYGINVIRSFPGRGIRIWG
                     VRSLAKAEADPVWQFVHVRRVMSMIEESVDESMQWTVFEPNDEALRKTVVHSLSVFLE
                     AIWQRGGLKGNVSSEGFYVKCDETNNPQSVIDAGQLVCEIGVAVAAPMEFLVFEIRRS
                     PSGAAVVEL"
BASE COUNT          506 a          589 c          602 g          543 t
ORIGIN      
        1 acgacacacc agggtgagac ctttgtgccg aaggaaaaca gtttgccggc tttcccccag
       61 agcagcacac accgtggtgg ggcgtgtgtt tcggagtgca ctttggtgag ttgatagact
      121 tgcccggtga gttctcgaac atcttttacg tcgtcaacca taccgtattg ggtggtatca
      181 aaaaagagat ccatcgtaat cttttccgcc tgcccgcgaa cgaactgcac tacaggagaa
      241 tcaaggccgg gaatggcgat ttcggcaaat tggacggctt tattgacggt gtatcgttcg
      301 gggttgaaca atgcatggat cggctggcca cgttcaggcg tgatctgtaa cttgtcgagt
      361 cccatgcggc ttcctttagc tcctctgata ggcttgattg tgtatcgccg tatcacgggt
      421 ccgttgcgtg atccgctctt gctcatggcg aaggtgctcg acaaccaagg ccacgatgcg
      481 cttgacatca tcagcactca gcgtaccgac tccttccgtg accacaatgt cggttacgac
      541 ctcaccgatt cgaatatctg gtccgtccat tttaagcccc cacctcgagc agcaatcctt
      601 gatgggaaat ctccaaggat tcgacggcta cattgctctg accggcattg agcgatgggc
      661 caacccactt catcggaatg ccttttttga atttccacac tttgacaggc ttgcgagctt
      721 cattgagcag aacgatcaat ccgtcctttc gtgtgccttt cccttgcaca aagtcatagt
      781 gccacgtcca gaggtcgtca tagagataga tcacgccatg ttttagggtg atgttggcgt
      841 gactggcgcg cgtgggaaac ttgtggacaa acccgttttg cccaccttcc ttgtactcaa
      901 acacttctac tgtggcgtcc aatccggaac attctgaaaa gccggcgacc ttgtaattca
      961 gcgcggccgt aatgagcgtc cctaccacat tcgaggaatc aatgagggta agatagaaat
     1021 taaagacgcc taaggggtcg attctgaatc ctgatgccat atgaatcgtt cctcaaagtt
     1081 cgacgactgc cgcaccgctc ggactgcgcc gaatttcgaa tacgagaaat tccatcgggg
     1141 ctgctacggc taccccgatc tcacatacca gctgcccagc gtcgatcacc gattgtgggt
     1201 tattcgtttc atcgcacttg acgtaaaatc cttctgacga cacgttgcct ttcaaaccgc
     1261 cgcgttgcca gatggcttcc aggaaaaccg acagcgaatg gaccactgtc tttctgaggg
     1321 cttcatcgtt cggctcgaac actgtccatt gcattgattc atccacggat tcttcgatca
     1381 tcgacatgac acgacgcacg tgaacgaatt gccataccgg atctgcttct gctttggcca
     1441 acgaccggac gccccagatc cgaatgccac gtccagggaa tgagcggatg acgttgatgc
     1501 cgtaaggatt caactgttcc tgctggaggg aagtgatctc ttgctgaacg tcggtgacga
     1561 actcaaatgg ctcattgcca ggtggacgtt ggacgccgaa ctggttatcg attcgggcat
     1621 agatgccggc tacatgcccc gatggtggaa gggggcgagg ggggcctgcc ggattggcgg
     1681 catcgggaac aaggaaccag ggataataga tggctccaaa gcgggtgatg aactggttgc
     1741 gccaagcgag cagttgtgcg gccgttggta ctgctctggt cggcagatag tcgagtatgg
     1801 ccactcgatc tctcaaacgc tcacactggt cgatcatcgc acgatatatc agcgtcgtct
     1861 ggtcagatgt ccaagacggt gacctcgacg ttttatcaac tggtagttcc ttggctagtg
     1921 gagttgatgg tgtcgcgcca ggatcgcacg ggtcgctaga tggtcgagga ggttctatag
     1981 ttgctctaga ggcaaaacat ggcactgcat cgggcaggca gaggatggat acttcatcaa
     2041 tctcctcgag caatcgaagt cctcgaagat cgtcggtacc tccaatgaga tcagatggag
     2101 tcacatcgtc cagaccgtct cgccctccct ggaggtattg agcctccagc ttccagatat
     2161 catcctgaac caagagaggt atggcagatg atgggatctc aatgtcagac gggtcctcaa
     2221 tttggccggt attatcggtg
//
LOCUS       DATMTI010000526         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_826713, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000526 DATMTI010000000
VERSION     DATMTI010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826713"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VN039_08435"
     CDS             <1..184
                     /locus_tag="VN039_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46030.1"
                     /translation="SWPVLMTSDITYSAVVAAKGANESGATISQTAGHGDFRLPTDFP
                     SRQVIIRLPSVSMRPR"
     gene            727..1296
                     /gene="pyrR"
                     /locus_tag="VN039_08440"
     CDS             727..1296
                     /gene="pyrR"
                     /locus_tag="VN039_08440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWV46031.1"
                     /translation="MMTTPLNKPVERKDEKLIMDAGDISRAMIRIAHEILERNKGVKD
                     LALVGIRTGGVHLAHRLVKRIHGIEGVQIPIGELDITLYRDDLALRKNQPILRKTSVP
                     FDMTNKVVVLVDDVLFTGRTIRAAMDGLMDLGRPEEIQLAVLIDRGHRQLPIKANYIG
                     KNLPTSRDEQVQVLLDEDGEDDRVVILKS"
     gene            1310..2230
                     /locus_tag="VN039_08445"
     CDS             1310..2230
                     /locus_tag="VN039_08445"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWV46032.1"
                     /translation="MSLKRKDLLSLAPLSVDEIRLVLDTADSFKEVTGREIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHSSAGAAETLANGVKSSVINAGDGWHEHPTQALLDLYTIRQRGMNFHGLRVA
                     IVGDVSHSRVARSNIYALIKLGAEVRLVGPPTMMPCGVEKLGVKVYSDMDEGLRGANV
                     IMMLRLQLERQGRALFPTIREYSRLYGLTAERVRLADAGAIVMHPGPINRGVEIAPDV
                     ADSLSSVILDQVANGVAVRMGILYLMSGAN"
BASE COUNT          534 a          594 c          674 g          536 t
ORIGIN      
        1 gagctggccg gtgttaatga ccagcgacat cacctacagc gccgtggtgg ccgccaaggg
       61 cgcgaacgag tcgggtgcca cgatttcgca gactgccggt cacggcgact tccgcctacc
      121 tactgacttt ccctctcgcc aggtgatcat tcgtttgccg agcgtgtcaa tgcgaccgcg
      181 atagccgagc cgctaaatcc ctgcctaaac gaggggcggc ttcacgtctg agagattcct
      241 tttctaagtg attgtcatga gggaaccttc cgtcggttcc cccatgacag agctccgacg
      301 agatctgtat atgtatgtga ttgacacgat cgtatgcagt cacctcgttc cttgaagcgt
      361 tgctcaagtt ttcaagataa gtaagtttca ctgccgtgtc cttgtcgccg tataaaagca
      421 gcgtcggcgg ttgtggggcc gtcaatgaac atggtgccct gcagactgac cgcaaacaga
      481 ttcctgaacg ttctggttcc taggtcacct cgtaaactat ttcattctgt cttcgaatac
      541 ttgacagttt tcaagtgttt cagtaggatg gcgaaacctt taatgctcat ccagtgaggt
      601 gagcaaggag ccaatcatga acttcggtcc ggccacggcc ggtgataatc gaccttctca
      661 caatcgcacg gtgaggaggt ttttttatgc cccgatttca caagaacttg ctcacatcgt
      721 tacttgatga tgacgactcc cctgaacaaa ccggtggaac ggaaagatga aaagctgatt
      781 atggatgccg gtgatatctc ccgtgccatg atacgtatcg cgcacgagat tttggagcgg
      841 aacaaagggg taaaggatct cgcactagtg ggaatacgga caggtggcgt gcatctcgcc
      901 catcgactcg tcaagcgtat ccacggtatc gagggcgtcc aaattccaat cggtgaattg
      961 gatatcacat tgtatcgcga tgatctggcg ttgcgcaaaa atcaaccgat tcttcgaaaa
     1021 acgtccgtgc cgttcgacat gacgaataaa gtcgtcgtgc tcgtcgacga cgttttgttc
     1081 accggaagga cgatacgagc ggcgatggat gggcttatgg acttgggacg tccggaggag
     1141 atccaactgg cagtgttgat cgatcgtggc catcggcaac tgccgatcaa agccaattac
     1201 atcgggaaaa atctaccgac ctctcgggat gagcaagtcc aagtgttgtt ggacgaagat
     1261 ggggaagatg accgggtcgt catcttgaaa tcctgaatcg gaggatccca tgagcctcaa
     1321 gcgcaaagat ctgctgagtc tcgccccatt atctgtggat gagattaggc ttgtcctgga
     1381 tacggcagat tccttcaagg aagtgacagg acgtgaaatc aaaaaagtgc cggctctccg
     1441 aggcaagacc gtcgtgaacc tcttttttga accgagcacc agaacccgca cgtcctttga
     1501 gctggcggcc aagcgtctga gcgccgacgt gatcaacttt tccccctcct ccagtagcgt
     1561 ggtgaaaggg gaaacgctcc ttgacacggc tcggaatatt gaagcgatgc aggccgacat
     1621 catcgtgctg cgccattctt ctgcgggggc cgcggaaacg ctggcgaacg gcgtcaaatc
     1681 gtcggtcatc aacgcaggcg atggatggca tgagcacccg acgcaagcgt tgctcgacct
     1741 atacacgatt cggcaacggg ggatgaattt ccatggattg cgcgtggcga tcgtaggaga
     1801 tgtgtcacat agccgggtgg cgcgctcgaa catttatgcg ctcatcaagc ttggtgccga
     1861 ggtacgtctg gtgggacctc ctacgatgat gccctgcggt gtggaaaaac tgggagtgaa
     1921 agtgtactcc gacatggatg aaggccttcg tggagcgaac gtcatcatga tgcttcggtt
     1981 gcaactggag cggcagggac gcgcgctgtt tccgacaatt cgggaatact cgcggctcta
     2041 tggcttaacc gctgagcgag tgcggctggc cgacgccggc gccattgtca tgcatccagg
     2101 gccgatcaat cgaggagtgg aaatcgcgcc ggatgttgcc gatagtttgt cctcggtgat
     2161 tctggatcaa gtggcgaacg gtgtcgcagt ccgtatggga attttgtacc ttatgtccgg
     2221 agcgaattga ggatgttgca aaaactctcc agcatcgttc tcgcatcgct caaggtctca
     2281 acgtaccaag cgggagagag ctgccccggc agctcggggt gggctgggtg ctaaagat
//
LOCUS       DATMTI010000527         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_529594, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000527 DATMTI010000000
VERSION     DATMTI010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529594"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2298)
                     /locus_tag="VN039_08450"
     CDS             complement(<1..2298)
                     /locus_tag="VN039_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWV46033.1"
                     /translation="MSERDAQFHYHVGRSTRNILSPLSRRRGAIHARAFTPARGASRP
                     VIRTSDATLFLILILTLALGLAGVGSLSPHSAHAVERNLTMSDSRADRGPGGAAKPDD
                     IDVATLRERRKQAQTDLNAVRESLASKSVTGMAREGQRERQALLEQIIRGYDEQLSDF
                     QRLTEARQRHTDIVLGSSEWKGFAEPSPYSIFLADQLWDTVHSLNLAAEGLQSQRELL
                     TLRFEQAREALTTAEERLRQASERFETAKDPGQADRERRNYELAALRARAASVLLEAA
                     QLSTRRVEEELDGTKAQLAVEERRLGAVLPHVAFSKTDLEQILIRLAEERVHLEAQLE
                     QTLSERQGLSQVVQEAERRLVLLRRKQPAGKEETPASARAKAEIELVQTRKDNLATQG
                     DLLQQLMNVVEGERQLWESRFVIAHASEPGKVREAYKRFAPLFDNFHFFRDYLRQQAG
                     IVSGQISELESRMSHTPASQHALLQDLVQAFHEREAVYTRTLHRMDEAARFMERWRAD
                     FKQQQKELPLFDRLQEWLTHVESLMNDAWGYELFSAEDTIEVDGKTITGRRGVTVGKT
                     ITALGIFLIGYAVCLYVARIIGGLSAARFGMTPDGANLVRQWTQAVLITFLIVLSFSW
                     AKIPLTIFAFLGGAVAIGVGFGAQTLLKNIISGILLLIERPMRVGDLIEADNVRGRVT
                     AIGLRSSTIRDAKGTETLIPNSSFLERALTNWTYSSRVGRFSLRVGAPYGTAAPQIMN
                     LLSALTLQHPKVMKQPAPQVLLDEFG"
     gene            2618..3886
                     /locus_tag="VN039_08455"
     CDS             2618..3886
                     /locus_tag="VN039_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV46034.1"
                     /translation="MMTDDVVTVAVVGAGAAGLTGAIVAAETLAKDGNPGRVLLLDVA
                     KQIGAKILVSGGGRCNVTHTVITPSDFFGNRNIIRNILAAFSVERTIAWFTSLGVDLK
                     RESTGKLFPTTDDARTVLAALLARARHAGVLIRPDHRVTKVSTSSQGFLVWHSHGRTQ
                     ASRVMLATGGQSLPRTGSDGFGYRLARALGHRVMPTVPALVPLVLDGSMFHSMLSGLS
                     HDVELTSLVDGREVDRRTGSLLWTHFGISGPVVMDASRFWTLATNRNAQVDLYGNFVP
                     GRTSEELKEWFVAQAVEHPRRSLTRTLSMLVPDRFADSLCRHCSCDSQELTAQVARRD
                     RERVLSTLTRFRFPIERDRGWNFAEVTAGGVPLEEINYRTMESKLVSGLYLIGEMLDC
                     DGRIGGFNFQWAWTTGWLAGQSAARSLPAA"
     gene            complement(3960..>4799)
                     /locus_tag="VN039_08460"
     CDS             complement(3960..>4799)
                     /locus_tag="VN039_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46035.1"
                     /translation="LDEVSMDWLKQCWKWFWDQSVFNKIVIIGLFAAFVPFVLPTVVA
                     WFRPTMVHVGVAFYTYERGIQPQGMNSLYFFERRNLVTDCSIRREEQSVPSGRAKVPD
                     PEAWVLIKLLLENVSDRDITNLRVGVRSPAINQTTQLLTAPTLAATGGKEAPPDVKPL
                     YVMSIATIPAASSVVVSLKTPMDENLREFIYVKRRPVTIQVPYVSADQFRQYPPIVSR
                     TNAVKMLNREGVLRTTDEAFADEKLQVTIVSPNGSNQNEREGAQRLLPRAKVCSEAEA
                     GVW"
BASE COUNT         1048 a         1370 c         1330 g         1051 t
ORIGIN      
        1 tccaaattcg tcgagcaaca cttgcggtgc aggttgtttc atgactttcg gatgctgtag
       61 ggtaagcgct gaaagcagat tcatgatctg tggggcggcc gtaccgtatg gagcaccgac
      121 gcgaagagaa aatcgtccga cacggcttga gtacgtccaa ttggtgagag cccgttcgag
      181 gaagctgctg ttcggtatca acgtttccgt tcctttggca tctctgatgg tagatgagcg
      241 gagaccgatc gcggtcaccc gacctcgaac attgtcggcc tcaataaggt cgccgacccg
      301 catcggccgt tcgatcaaca gcagaatccc gctgataatg tttttcaaga gtgtctgggc
      361 accgaatccc acaccgatcg caacggctcc gccgagaaac gcaaaaatcg tcaaagggat
      421 cttcgcccat gagaagctca gcacgatcaa gaaggtgatg agaactgctt gagtccactg
      481 gcgcaccaga tttgcgccat ccggagtcat gccgaacctc gctgcagaca gcccgccgat
      541 gatccgcgcg acatacagac aaacggcgta gccgatgaga aagattccta acgcggtgat
      601 cgttttgccg accgtcactc cacggcgacc ggtgatcgtt ttaccgtcca cttcaatagt
      661 atcttccgcc gagaaaagtt cataacccca cgcatcattc atcaggcttt cgacatgggt
      721 gagccactct tgaaggcgat cgaacagagg taattccttc tgttgttgtt tgaaatcagc
      781 ccgccatctc tccatgaacc tcgcagcttc atccattcgg tgcaacgtgc gcgtgtagac
      841 cgcttcccgc tcatgaaatg cttgtacgag gtcctgcaat agggcatgtt gtgacgccgg
      901 cgtatggctc atccgactct cgagttcact gatctggccg gacacgattc cggcctgctg
      961 gcgcaaataa tcccgaaaaa agtggaaatt gtcgaaaagc ggcgcgaacc gtttataggc
     1021 ttcccgtacc ttccccggct cggatgcatg agcgatgacg aagcggctct cccatagttg
     1081 gcgctcgccc tccacgacat tcatcaattg ctgcaagagg tcgccttgcg tcgccagatt
     1141 atccttacgg gtctggacca attcgatttc cgcctttgct cgagccgacg ccggtgtctc
     1201 ctcctttccc gccggctgct tgcggcggag aaggaccaat cgccgttcgg cttcctggac
     1261 aacctgagac agcccctgcc gctcggagag ggtttgctcc agttgtgctt ccagatgtac
     1321 acgttcttct gctaatcgga tcagaatctg ctcgaggtcg gttttactga aggcgacatg
     1381 tggcaagact gcgccaagcc tccgctcctc cacagcgagc tgcgccttgg tgccatccaa
     1441 ttcttcttcc actcgccgtg tcgaaagttg tgccgcttcc agcagcaccg aagcagcccg
     1501 agcgcgaaga gcggcgagtt cgtaattccg gcgttcacga tcggcttgtc cggggtcttt
     1561 ggctgtttcg aaccgttccg aggcctgccg cagccgctct tcggccgtcg tcagcgcttc
     1621 acgtgcttgc tcgaatcgca acgtaagtaa ttcacgttgg gattgcagac cttcggcggc
     1681 aaggttgaga gaatgtacgg tatcccacaa ttgatccgcc aggaagatcg aatagggtga
     1741 aggctcggca aaacctttcc actcactgct gccgaggacg atgtcggtgt gccgctgacg
     1801 cgcctcggta agccgctgaa aatcgctcaa ctgctcgtca tacccacgaa taatctgctc
     1861 cagcaatgcc tgccgttccc gctgcccctc tcgggccatc ccggtcacgc tcttggaagc
     1921 gagagattct ctcacggcat tcaggtccgt ttgcgcttgc ttgcgccgtt cgcggagcgt
     1981 cgccacatct atgtcgtccg gcttagcggc gcctcccggc cctcgatcgg cgcgagaatc
     2041 cgacatcgtg agattccgct cgacggcatg agcggaatgc ggggacaacg aacccactcc
     2101 agccaggccg agtgcgaggg tcaggataag aataagaaat agagtagcat cggaggtgcg
     2161 gatgactggc ctggaagctc cgcgtgccgg agtgaatgcg cgggcgtgta tagctcctcg
     2221 ccgccgactg agaggggaca aaatatttcg cgtcgaacga ccgacgtgat aatgaaattg
     2281 ggcatcacgt tcgctcatga acggcacaaa ccgatccgct aaatgctaca acgtcgacgt
     2341 ggacttaagc tggggctagt cgttgaggtg gcctagacta tgtcttattc taataccgtt
     2401 tcctcataat cacactaggg caacccctag ttactgattc aactccgata ggggcgacta
     2461 atctctttgt gactgagagg aggcactgaa agggcatgaa gggacaacat ccgagtaaca
     2521 agtagtatca aaatagtaac aaaaccgtat taccctacag aatagaagtt taggcaataa
     2581 tgagtccaac attcgccgga tagagcacga agatcagatg atgactgacg atgtggtgac
     2641 cgtggccgtc gtgggagccg gcgcggcagg actgaccggt gcgatcgtgg cggcggaaac
     2701 cctcgcaaaa gatggaaatc ctggacgtgt gctcttgctg gatgtggcca agcagatagg
     2761 agccaaaatt ctggtctcag gcggcgggcg ttgcaacgtc acgcacacgg tgatcactcc
     2821 aagtgatttc ttcgggaatc gaaacatcat ccgaaatatt ctcgctgcgt tttcagtcga
     2881 acggacgatc gcatggttta cctcgttagg tgttgaccta aagcgcgaat cgacaggcaa
     2941 gctgtttccc accaccgacg acgcacgaac cgtcctcgcc gcattgttgg ctcgtgcccg
     3001 acatgccggc gtcctcatac gtccggacca tcgtgtgacc aaggtatcga cgtcaagcca
     3061 aggatttctg gtgtggcaca gtcacggccg aacgcaagca tcccgcgtca tgctcgccac
     3121 aggcggtcaa tcattgccga ggaccggcag cgacggattc ggatatcgac tggctcgtgc
     3181 tcttggccat cgcgtgatgc cgacggtgcc ggccctggtc cccttggtgc tagatggttc
     3241 catgtttcac tcaatgctct caggcctatc gcatgacgtg gagttgacct ccctggtgga
     3301 tgggcgagaa gtcgatcgac gcaccgggag cctactctgg acccatttcg ggatcagcgg
     3361 ccccgtggtc atggacgcca gccgtttctg gaccttggcg acgaatcgaa atgcacaggt
     3421 tgacctttac ggaaactttg ttccagggag gacgtcggaa gaattgaagg agtggtttgt
     3481 cgcccaagcg gttgaacatc ccagacgctc attgacgcga acactgtcta tgctggtccc
     3541 ggatcgattt gccgactctc tctgccgcca ttgctcatgc gactcgcaag agctaacggc
     3601 tcaagtcgcg cgtcgtgatc gagagcgggt actgagcact ctgacgaggt tccgatttcc
     3661 catcgagcgg gaccgcggat ggaactttgc ggaagtcacg gccggcggcg tgccgttgga
     3721 agaaatcaac taccgcacca tggagtcgaa gctggtttca ggactctatc tgatcgggga
     3781 gatgctcgat tgcgacggtc gcatcggagg gttcaatttt cagtgggctt ggacgacggg
     3841 ctggctggcc ggtcagtcag cggcgaggag ccttccggcc gcgtaggctc cacctcactg
     3901 cacatgatcg acggcgtggc tcgttccttg gtttgattcc gactctcctg agacgaaact
     3961 caccacacgc ctgcttctgc ttctgaacaa acctttgctc tcggaagaag tcgttgtgct
     4021 ccctctcttt cattctgatt cgacccattg ggtgacacta tcgttacctg gagtttttcg
     4081 tcagcgaagg cctcgtcggt ggtccgcaac actccttcac gattgagcat cttgaccgcg
     4141 ttcgtgcgcg agacgatcgg tggatactgt ctgaattgat ctgctgagac atagggtacc
     4201 tgaatggtca cagggcggcg tttgacatag ataaactcac gcaagttttc atccatggga
     4261 gtcttcaaac tgacgaccac cgagctcgcc gccggaatgg tcgcgatgga catgacatac
     4321 aaaggcttca cgtcgggagg ggcctccttg ccgcccgtgg cggccaaggt tggagcggtc
     4381 aacagctgcg tcgtctgatt gatagcgggt gaccgcacac cgacccggag attcgtgata
     4441 tcgcgatcgg acacattttc cagcagcaac ttgatcaaca cccacgcttc cggatcaggc
     4501 accttcgcgc gaccactcgg gacggattgc tcctcacggc ggatactgca atccgtcacc
     4561 agattgcgcc tttcgaagaa atagagtgaa ttcatgccct gtggctggat accccgttca
     4621 tacgtgtaga aggccacgcc gacgtgaacc attgtcggcc tgaaccaagc cacgaccgtc
     4681 gggagaacaa atgggacaaa agctgcgaac agcccaatga tgacgatctt attgaagaca
     4741 ctctgatccc agaaccactt ccagcactgc ttcaaccaat ccatggacac ctcatcaag
//
LOCUS       DATMTI010000528         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_317237, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000528 DATMTI010000000
VERSION     DATMTI010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317237"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VN039_08465"
     CDS             <1..694
                     /locus_tag="VN039_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWV46036.1"
                     /translation="SEMNTLEDKLITIEDPVEYQLSGVVQIPVNEKKGVTFARGLRSI
                     LRHDPDKIMVGEIRDAETAQIAIQSALTGHLVLTTVHANNVFDVIGRFASMGIDTYNF
                     LAALNCVLAQRLVRILCPSCRTPVKGQQALIEESGLDYEQHKDTPFYEGRGCPQCHGT
                     GYRGRKCITEFLDLTDEIKEMIHAERPLAEIRYRAVTDGMITLRQSAMTKVLNGETSL
                     REINRVTFSEEG"
     gene            735..1694
                     /locus_tag="VN039_08470"
     CDS             735..1694
                     /locus_tag="VN039_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46037.1"
                     /translation="MKFGTDSIAWAETERNWRGQRRHTCIMSPLSDGIIKPSPTEENV
                     SDPSALADRIRVLTRSGSVHHAVGGAVLSDLPRRIGVLLPDTAVRATVLHLEQLPVRR
                     EERDALIRWRLGQEQLFPLSGAKLLSQVFHDPSGGKGPAYTVLTVAAQESMLKQYESL
                     CESVGLIPQEVGITSLRIFDLWRRALGGSHWRSRECLWVNVSDRALTTMICQRGRLLF
                     YRCKLLGVEAAEALTKTDMLNKILEECWASVEICQQQHPSAVVKELVICADGDISAFQ
                     ELVEAELRLSVEQLGWQSVEAIGRVTKRSHRGMASLAAMAGVL"
BASE COUNT          397 a          421 c          531 g          387 t
ORIGIN      
        1 atcggagatg aacacgctcg aagacaaatt aattacgatc gaagatcccg ttgaatacca
       61 actctcggga gtggtacaga ttcccgtcaa tgaaaaaaaa ggagtcacgt ttgctcgcgg
      121 ccttcggtcc atactccgtc atgatccgga caagattatg gtcggcgaaa ttcgagatgc
      181 cgaaacggcg cagatcgcta tccaatcggc cctgaccggc catcttgtgt tgacgacggt
      241 gcatgcgaac aatgtctttg acgtcatcgg gcggttcgcg tcgatgggga tcgacaccta
      301 taattttctc gccgctctca actgtgtgtt ggcgcagcgg ctggtccgaa tcctgtgccc
      361 gtcatgtcgg actccagtca aaggacagca ggcccttatt gaagaatcag gattggacta
      421 cgagcaacac aaggatacgc cgttctacga aggaaggggg tgcccgcaat gccatggtac
      481 cgggtaccga ggaagaaaat gcatcacgga attcctcgat ttgacggatg aaatcaagga
      541 aatgattcat gccgagcgac ctcttgcaga aatccggtat cgggccgtga ccgatggaat
      601 gattacgctg cggcagtcgg cgatgacaaa agtgttgaat ggggagacgt cgctgcgaga
      661 gatcaatcgt gtgacgttca gtgaggaagg gtaatgcgat gtgggaatgg gctagtagcc
      721 gaccacagcg ttgcttgaag ttcggaaccg actcgatcgc gtgggccgaa accgagcgaa
      781 attggcgtgg gcagcgtcgc catacgtgca tcatgtcccc gctttccgat ggcatcatca
      841 aaccctctcc gacggaggag aatgtatccg atccctcagc attggcggat cggattcgtg
      901 tcctgacgcg ttcaggctcg gttcaccatg ctgtcggggg agcagtcctc tctgatcttc
      961 ctcgacggat tggagtattg ctgcctgaca cggcggttag agcgacagtc ctgcatctcg
     1021 agcagctccc tgtcaggcgc gaggaacgcg atgcgctgat ccgctggagg cttggtcaag
     1081 agcaactgtt cccgctgagc ggcgcgaagc tattgtccca ggtatttcat gatccctcgg
     1141 gaggcaaagg gccggcctat actgtgctga cggtggcggc ccaggaatcg atgctgaagc
     1201 agtacgagtc tctgtgtgaa tctgtcgggt tgatccctca agaggtggga atcacgagcc
     1261 tacggatctt tgatctatgg agaagggctt taggtggatc tcattggcga agccgagagt
     1321 gtctatgggt caacgtatcg gatcgagcct tgacgaccat gatttgtcaa cgaggacgct
     1381 tgctgttcta ccgatgcaag cttctggggg tcgaggcggc tgaagcgctg acgaagaccg
     1441 acatgttaaa caagattctt gaggaatgct gggcatcggt ggagatctgt caacaacaac
     1501 atccttcagc ggtcgttaag gaactggtga tttgtgcaga cggggacatt tcggcctttc
     1561 aggaattggt tgaggcagag ctccgactgt ccgtcgaaca actaggttgg caatcggtcg
     1621 aagccattgg tcgggtgacc aaaaggagtc atcgagggat ggcatcgctg gctgcgatgg
     1681 ctggggtgct ctagcatggc gatcatgagt tctgcagtcg ctaggttctt cgaacc
//
LOCUS       DATMTI010000529         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_656537, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000529 DATMTI010000000
VERSION     DATMTI010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656537"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..726)
                     /locus_tag="VN039_08475"
     CDS             complement(274..726)
                     /locus_tag="VN039_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HWV46038.1"
                     /translation="MSVERDIVEGVAELIDAAGLATFHPSGVYADSETGIVFKVVPAK
                     PDRVVILTAVPMTDDISMPLGQVMLQVRTRGLPNNPLDVDDLAGPIFDLLQGRTYDTF
                     GDTTVIQMRRTTSIPNGQDAAERFERIDQFYLDCDVNPTVNRPFWGSW"
     gene            complement(723..1064)
                     /locus_tag="VN039_08480"
     CDS             complement(723..1064)
                     /locus_tag="VN039_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46039.1"
                     /translation="MSIEWDIDLHLEEISADVLARVPVALGKGLEFLRAEVTPKVPVE
                     TGNLAGSGAVSVQGSEGELLYPGPYAQVQHFDLSFHHNTGQALYLEQPLVQSAEAVVK
                     VIGDELGGALE"
     gene            complement(1073..1408)
                     /locus_tag="VN039_08485"
     CDS             complement(1073..1408)
                     /locus_tag="VN039_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46040.1"
                     /translation="MTGGVARWFVHTVVVETFEGAGAYGNVYAAPVTLAPPNGCWVDG
                     ARKLVRNSAGVEVVSETSLATFKANAPLFTPDSRVTINGVVSLVIKTNEADSGSLNLP
                     TDHVEVNLT"
     gene            complement(1405..1857)
                     /locus_tag="VN039_08490"
     CDS             complement(1405..1857)
                     /locus_tag="VN039_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46041.1"
                     /translation="MPAYYGNFVVPETLAAPEDLAAWTGTTAPSNATVLLRSATSLVL
                     EATRQAYYDVDPLTGLATDTQIGGALQNATVIQAAAWAALDYDPLTGGVITAGVESST
                     KMLSATIVFADASQAAQSRSDAVTGLVPEALRVLELNNLLIPNPWAFG"
     gene            complement(1869..2162)
                     /locus_tag="VN039_08495"
     CDS             complement(1869..2162)
                     /locus_tag="VN039_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46042.1"
                     /translation="MSKTVHILGEGGGIFEMSLPLSEPIEDRLLKGYLKRVNADGTPY
                     REKSERVQPAPYASKGEWVGWAVHVSAHTDSPLTPDDAEALTKQDLIEQYGVK"
     gene            complement(2162..>2416)
                     /locus_tag="VN039_08500"
     CDS             complement(2162..>2416)
                     /locus_tag="VN039_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46043.1"
                     /translation="ANVVLLRVSNASGGSGTVSVLGGVQPLAPSSGQGAATATVANGG
                     TSWLGPFESARFAQADGSYLVESSVIMTVTAFAVDGRRSA"
BASE COUNT          578 a          743 c          627 g          468 t
ORIGIN      
        1 cagtccacgt ctgcatcgtc ttctcgaacg acgcccaacc gtcagagtcg tagtcgtctg
       61 ctgactggtt cgttggggag atggacgggt tgaggtcgtt gacccctttg aaatcaatcc
      121 aggcggtgtt atccgcagac acttgcagct tgaaccttcg ggccaaggct gttgacatta
      181 tttgcctcct aaggcggtta aatgaaaaaa gacccgttag ggtcttcgtg ttgccgttac
      241 cggcacccgc ccggacaggc ggaggaataa gggctaccag gaaccccaga agggccggtt
      301 gacagtcggg ttcacgtcgc aatcgaggta gaactggtcg attctctcga accgttcagc
      361 ggcatcctga ccgttcggaa tcgacgttgt acgccgcatc tgaatgacgg tcgtgtcccc
      421 gaacgtgtca tacgtgcgac cctgcaacag gtcaaagatt gggccggcca aatcatcaac
      481 atcaagaggg ttgttcggca gaccgcgagt acgaacctgc aacatcacct gacccaacgg
      541 catcgaaatg tcatccgtca tcggcacagc ggtgaggatc acaacccggt caggtttcgc
      601 aggcacaacc ttgaacacaa tgcccgtttc cgaatctgcg tacactcccg aagggtggaa
      661 cgttgccaac cctgccgcgt cgatcagttc ggcaacacct tcaacaatgt ctcgttcgac
      721 actcattcga gcgcaccccc gagttcatcg ccaatgacct tcactaccgc ttccgcgctt
      781 tgcaccaacg gttgttccaa gtacaaggct tggccggtgt tgtgatggaa agacaggtcg
      841 aaatgttgga cctgagcgta cggaccgggg tacaacagtt cgccctcgga accttgaaca
      901 gacacagcac cggaccccgc aaggttcccg gtctcaacag gtactttcgg ggtgacctcg
      961 gcccgcagga actcgagacc cttaccaaga gcaacgggta ctcgcgctag cacgtcggct
     1021 gagatctctt ctaaatggag gtcgatatcc cattcgatcg acatgcggcc tcctaggtga
     1081 ggttcacttc aacatgatcc gtaggcaaat tcagtgaccc cgaatcggcc tcattcgttt
     1141 tgattaccaa cgacacaaca ccgttgatcg tcacccgtga atccggggtg aacaaaggtg
     1201 cgttcgcttt gaacgtggca agactggttt cggacacaac ctcaacaccg gcactgttac
     1261 gcaccagttt tcgggcacca tccacccaac aaccattcgg cggggccaac gtcacagggg
     1321 ctgcgtacac gttcccgtac gcaccagcac cctcaaacgt ttccacaaca actgtgtgca
     1381 caaaccagcg tgcgacaccc ccggtcatcc gaacgcccaa gggttaggga tcaacaagtt
     1441 attcagttca aggacgcgta aagcttccgg tacaagaccg gtcacagcat ccgaacgaga
     1501 ctgtgccgcc tgcgacgcgt cagcgaacac aatcgtggcg ctgagcattt tcgtagacga
     1561 ctcaacccca gcagtaatca caccaccagt caacgggtca taatccaatg ccgcccacgc
     1621 tgccgcctga atgaccgtcg cattctgtag cgcaccacca atttgcgtgt cagttgctaa
     1681 cccggtcaaa gggtccacgt cgtaatacgc ttgacgtgtc gcctccaaca caagagacgt
     1741 ggccgaccgc aacaacacag tcgcattcga cggtgccgta gtaccggtcc acgcggccaa
     1801 atcttcaggt gcagctaacg tttcgggaac aacaaaattg ccgtaatacg ctggcataag
     1861 atgcccctct acttgacgcc gtactgctca ataagatcct gtttcgtcaa cgcttcagcg
     1921 tcatccgggg tcagggggga atccgtgtga gcggacacgt gcacagccca accaacccat
     1981 tcacccttag aggcgtacgg ggcaggttgt acacgttcgg acttctcccg gtaaggggta
     2041 ccgtccgcgt ttacacgctt caggtacccc ttcaggagac ggtcctcaat cggttcggaa
     2101 aggggcaacg acatttcgaa aatgccgcca ccctccccaa gaatgtgaac cgtcttagac
     2161 atcaggcgga acgccgccca tcaaccgcga aagcggtaac ggtcatgatg accgaggact
     2221 ccacgaggta ggatccgtca gcctgcgcga aacgcgctga ctcgaaaggt ccaagccaag
     2281 aggttccgcc gtttgcgaca gtcgccgtag cagcaccctg gccactcgac ggtgcgagcg
     2341 gctgaacacc accgagtacg gaaaccgttc ccgacccacc ggacgcgtta gaaacacgca
     2401 gcagaacaac gttcgc
//
LOCUS       DATMTI010000530         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_784059, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000530 DATMTI010000000
VERSION     DATMTI010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784059"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2286)
                     /locus_tag="VN039_08505"
     CDS             complement(<1..2286)
                     /locus_tag="VN039_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWV46044.1"
                     /translation="MTLKETFERLDRLQSHVELRPSQLSILDEMDRRIDDRDLVLKMG
                     TGNGKTIVGLLYLQRHMQKSRSLVVYLVPTLQLIDQVIADGKSVGINVEAWTDRVAPD
                     RAIRGEAVLVCTYAKFFNGRSVFARSTMPLVPEAMVLDDVHAGIEAVRGNFSAKLSAE
                     TRIDLLELMGQELRAQVPGLMAGVEKKDDAALIEVPYWIIKNHLPRITELLAAAAEEE
                     DSDLLFAWPHLVDRLELARFFVTGTRAEVALDPPAVDLVRHYQQCKHRLFMSASIQDG
                     SALIRELGCAPTAANVPLEPTTDAGVGERLILTTSLVDPDLHRDSVVSLCKEMSRETN
                     VVVFVASEVAARPWVAAGATHPSTKEISETIHRLRNSHLGNFVVMVQRYDGIDLPDNA
                     CRLLVVDGIPTGDSLSDRVDLQLFGSVVGIRGKIANRLEQGLGRGVRSSSDYCAVLLV
                     GRDLGNFVSHRATIDHLSPQVAKQLEISREISGSAREGSTDSVQAIGAAIRQCLRRDS
                     GWKAYYAGEMRAVSSTAEQVTTQSTMVKVATLEREGLRKATSRNFREASKALEVAASE
                     LVDQPLSAGALLEHAARYMHHADHVAAQSLQRRAFEANQNVARPLQMHLRDVIKITSQ
                     AMNLAAWLKDFSDPNGPAIEIRSLRAALSFANPVRRVEEALRDLGRLLGANSTMPERE
                     FGRGPDVLWHFGEVTLVIEAKTDNRTKLHKTDAAQLLLGHTFVEEQLPRGQKFVAVIA
                     SNTEEVDRFEDWTFGAKLLSET"
     gene            complement(2429..2998)
                     /locus_tag="VN039_08510"
     CDS             complement(2429..2998)
                     /locus_tag="VN039_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWV46045.1"
                     /translation="MSEIVGYARVSSQGQSLAVQDEKLKAFGCTRIYAEKKSGRQQDN
                     RPQFQACLEFLREGDTLVISRLDRMARSVLDLAKIADVLKRKGVTLKVLDQSIDTGTS
                     EGKLMFSLLGAFAEFECDIRAERQSDGIAKAKASGVKFGRKKALTAEQVERIHVLRDE
                     EKLSVPDIAKRFNCGVATIYRNLSSASDQ"
     gene            3261..3590
                     /locus_tag="VN039_08515"
     CDS             3261..3590
                     /locus_tag="VN039_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV46046.1"
                     /translation="MSLDLSMKSAAESMGISASHLSGIEYGEKRLAQKHIDAAVAYFA
                     QSGASDTQLAVIQQASEKSKDVIHTTDLNPDARGLVAAFARRLQEGDAPTPEILSWLD
                     DPSRKRR"
     gene            3594..>4111
                     /locus_tag="VN039_08520"
     CDS             3594..>4111
                     /locus_tag="VN039_08520"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HWV46047.1"
                     /translation="MTTLGSSQSLRTPTGLKVAPMSYAKLEEVAEGLRHLLPSASGHG
                     GGPWKIDAWRILEKTLPQAGFGYHIAEIEDLKECAAFTIPEQRLVVLRQDIYEGVFKE
                     DVFARSTVVHELAHIVLNHAVTLNRGAAIGKHKFCEDSEWQAKALTAALMMPIEACKA
                     AHSATELAAMCGT"
BASE COUNT          962 a         1134 c         1093 g          922 t
ORIGIN      
        1 ggtctcagat agtagtttcg ctccaaaggt ccagtcttcg aagcgatcta cctcctcagt
       61 gttcgaggca ataacggcaa cgaatttctg acctctcgga agttgctctt cgacaaatgt
      121 atggcctagc agcagctgtg cagcgtctgt cttatgcagc ttcgtcctat tgtccgtttt
      181 ggcttcaatg acaagcgtga cttctccgaa gtgccagagc acgtctggtc cccgtccgaa
      241 ctctcgctct ggcattgtcg agttagcacc aagaagccta cctaggtctc ggagtgcctc
      301 ctctactcgt cgaaccgggt ttgcgaagga tagggcggct cggaggcttc tgatctcaat
      361 tgcagggcca tttgggtctg agaaatcctt aagccacgct gcgaggttca tggcctgaga
      421 agtaattttt atcacgtctc gcagatgcat ttgaagcggc ctagccacgt tctgattggc
      481 ctcgaacgct cgcctctgca gcgattgggc agcaacgtga tctgcgtggt gcatgtagcg
      541 agcagcatgt tccaacaatg ctccagccga cagtggttga tccacaagct cgcttgccgc
      601 gacttccagc gccttagagg cctcacgaaa atttcgacta gtcgcttttc taagtccctc
      661 tcgttcgaga gttgcaacct tgaccattgt cgattgcgtt gtgacctgct cagcagttga
      721 ggagaccgca cgcatctcgc cagcgtagta ggccttccag cctgaatcac ggcgaagaca
      781 ctggcgaata gcagcgccga ttgcttgaac tgagtcggtt gatccttccc gtgcactgcc
      841 ggaaatctcg cgagagatct caagctgctt agcaacctgt ggtgacaagt gatcaatcgt
      901 tgcgcgatgc gagacaaaat tgcccaaatc tcgtccaaca agcagaaccg cacaatagtc
      961 tgaactggag cggacgccgc gccccagtcc ttgctcaagc ctgttcgcaa ttttccctct
     1021 gatgcccacc actgagccaa agagctgaag atcgacgcga tcagacaggc tatcgcctgt
     1081 cggaatgcca tcaacgacaa gcagacgaca ggcgttgtcg ggcagatcaa tgccatcgta
     1141 tcgttgcacc atcacaacga agttcccaag gtggctgttg cgcaatcggt ggatcgtctc
     1201 cgagatttcc ttggtcgaag gatgcgtcgc gccagcggca acccatggcc gcgccgctac
     1261 ttccgaggca acgaaaacca cgacatttgt ttcacgactc atctccttgc acagcgaaac
     1321 cacgctatcc cgatgaagat cgggatctac aagcgatgtc gttaagatta ggcgttcacc
     1381 aacgccggca tcggtcgtgg gctcaagtgg aacattggcg gcggtcggcg cgcatccaag
     1441 ctcacggatt agagcgctcc cgtcttggat ggaagccgac atgaacaggc gatgcttgca
     1501 ctgttgatag tgacgaacga ggtcaactgc tggaggatct aacgcgacct ctgctctggt
     1561 gccggtcaca aagaagcggg ccagctctag gcggtctacg agatgtggcc atgcaaacag
     1621 gaggtcggag tcttcttcct ccgcggcagc agctaggagc tccgtgattc tagggagatg
     1681 atttttgatg atccaatatg ggacttcgat aagagcggcg tcgtccttct tctcaactcc
     1741 agccatcagg ccgggaacct gcgctcggag ctcttgtccc attagctcca gcagatctat
     1801 acgggtttcg gcggaaagtt tggctgagaa attgcctcgg acggcctcaa tacctgcgtg
     1861 cacatcgtcc aacaccatag cttcgggaac taaaggcata gttgagcggg cgaacactga
     1921 tctcccgttg aagaactttg catatgtgca gactaggaca gcctcgcctc tgattgcccg
     1981 atctggagcg acacgatctg tccaagcctc gacatttatg ccgacacttt tcccatcggc
     2041 aatgacttga tctatcagtt ggagagtggg cacaagatat acaactagcg agcggctctt
     2101 ctgcatgtgt ctctgcagat ataggagtcc aacgatggtc ttgccgttac ctgttcccat
     2161 cttcaggacc aaatcgcgat cgtctatgcg ccgatccatt tcgtccaaaa tgctcagctg
     2221 acttggtcga agctccacat ggctctgcag gcggtcgaga cgctcgaaag tctcctttag
     2281 tgtcaaaggt ggttcacctg ccttttgcct ttgcagctta ctgaaatcaa tcacgtcgcc
     2341 tccgatgcct cgtccaagac ctagcaaact gcgatagaac cgtccaaacc gcagactaac
     2401 gtagctttga ggcacggcca aatgatcgtc actggtcaga cgcagagctc aggtttcgat
     2461 agatcgtggc gacgccacag ttgaaccgct tggcgatatc cggaaccgac agcttttcct
     2521 catcccgcag gacgtggata cgttcgactt gctcagcggt aagagctttc ttccggccaa
     2581 acttcacccc tgaagctttc gccttggcga taccgtcact ttgacgctcc gcccggatgt
     2641 cacattcaaa ttccgcgaag gcaccaagca aggagaacat cagcttgcct tctgatgtgc
     2701 cagtgtcgat cgactgatcc agcaccttca gggtcacgcc tttgcgcttg agtacgtcgg
     2761 cgatcttggc caagtccaac acgctgcgcg ccatgcgatc gaggcgcgaa atgaccaacg
     2821 tgtcgccttc acggagaaat tctaggcagg cctgaaactg gggacgattg tcctgctgcc
     2881 gtcctgactt cttctcggca tagatccgag tgcacccgaa agccttgagc ttctcatctt
     2941 gaacagcaag agactgacct tggctgctca cacgtgcgta cccgacgatc tcactcatat
     3001 cgtatctcct aagaggttac gataatacgc ttttgagatg acttttgata gacctttccg
     3061 aggattccgg aaggtataca tatagagagg catccgccgc cgaggcctcc ccaagcacgg
     3121 gtatagtgcg cagaaagaga agatcttctc tttttggaga agcaggagta tagtgcgagg
     3181 tgaggatcga ccctcagcca atcaggacac gaattgctat gaaactgacg ccgttcggag
     3241 aagcagcacg gacactccgc atgagcctcg acctgtcgat gaaatctgcc gccgagtcca
     3301 tggggatcag cgcatcacat ctaagtggaa ttgagtacgg tgagaagcga ctcgcacaaa
     3361 agcatattga tgctgctgtc gcatattttg cacagagtgg agcaagcgac acacagcttg
     3421 cggtgattca acaagcatcg gagaagtcta aggacgtaat acacacgacc gatctgaatc
     3481 ctgacgcacg aggcttagtt gccgcgttcg ctcgaaggct acaagaaggt gatgctccga
     3541 cgccggagat cttaagttgg ctggacgatc catcacgaaa aaggaggtga tcaatgacca
     3601 cgctaggctc gtctcaatcg ctgcgcacgc ctactggctt gaaagtcgcg ccaatgagct
     3661 atgcgaagct cgaagaagta gccgaaggac ttcgccatct ccttccaagc gcttcgggcc
     3721 atggcggcgg gccttggaaa attgacgctt ggcggatcct tgagaaaacg ctcccgcagg
     3781 caggattcgg atatcacatt gcagagattg aagacctcaa ggaatgtgcc gcgtttacga
     3841 tcccggaaca acgactggtg gttttgcgac aagacatcta cgagggagtg ttcaaagaag
     3901 atgtttttgc gcgcagcacc gtagttcacg aattggccca cattgtcctc aatcacgccg
     3961 tcaccttaaa caggggcgcc gccatcggaa agcacaagtt ttgtgaggac tcggagtggc
     4021 aggcgaaggc gttgacagca gcgttaatga tgccgattga agcatgcaag gctgcgcatt
     4081 ccgcaactga gctggcggcc atgtgcggaa c
//
LOCUS       DATMTI010000531         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_233578, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000531 DATMTI010000000
VERSION     DATMTI010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233578"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VN039_08525"
     CDS             <1..398
                     /locus_tag="VN039_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46048.1"
                     /translation="RHLFEDPVEVVVCDGFVGNVILKTCESIAVAMFKWLKHELMRTP
                     MRKVGALLAKNAIGTIRDRTNYEEYGGSPLLGVNGICIIAHGASTPLAVKNALRVATE
                     SIEQQVNPHIVAEIRRYHETTAPLEPALS"
     gene            361..1383
                     /locus_tag="VN039_08530"
     CDS             361..1383
                     /locus_tag="VN039_08530"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWV46049.1"
                     /translation="MKRQPRSNPRSHKKLRTVSIIGTGSYVPEKVLTNEDLSRMVDTS
                     DEWITTRTGIKERRVAAKDEYTSDMAVKAALNAIEQAKISPKEIDLILLATATPDMLF
                     PATACFVQKKIGATNAACLDISAACAGFIFGLEIAQQFITSHTYDTVLVIGADKLTSI
                     TNWTDRNTCVLFGDGAGAAILRHRGSPHGIITTHIGSDGQYTNILFMPGGGSRCPITR
                     ENADMNLTTIHMTGKEVYKQAVIAMLNAAKKALEQAGLSVEDIACVIPHQANLRIMEA
                     IADRLGISRDKMFVNLDRYGNTSAAAVAIALDEANRSGRIKPGDYILMVVFGGGLTWA
                     STVIEW"
     gene            complement(1398..>1908)
                     /locus_tag="VN039_08535"
     CDS             complement(1398..>1908)
                     /locus_tag="VN039_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV46050.1"
                     /translation="GVPGIVRNRKDKLTEETIIMTTKTDTIRLHRVLRATPEKIYKAF
                     LDPDAMAKWLPPNGFTGKVHEIDAKVGGTYKMSFTNFTTGKSHAFGGTYIELTPHERI
                     KYTDKFDDPNLPGEMQTSIKLTKVSCGTELNVVQEGLPAVIPAEACYLGWQESLALLA
                     KLVEPEIPD"
BASE COUNT          430 a          516 c          537 g          425 t
ORIGIN      
        1 gccgtcacct ctttgaagat ccggtcgaag ttgtggtgtg cgacggattc gttggcaacg
       61 tgatcctgaa gacgtgcgaa tcaatcgcgg tagcgatgtt caagtggctc aaacacgaac
      121 tcatgcgcac acccatgcga aaagtgggcg ctctgctggc gaagaatgcc attgggacga
      181 tcagggacag gacgaattat gaagagtacg gcggcagtcc gcttctcggt gtgaatggga
      241 tttgtatcat cgcccacggc gcgagtacgc cgctcgcggt caagaatgcg ctgcgtgtgg
      301 ccaccgagtc cattgaacag caggtgaacc cgcatatcgt cgcggagatt cgccggtacc
      361 atgaaacgac agccccgctc gaacccgcgc tctcataaaa agctccggac ggtctcgatc
      421 attggtaccg gaagttacgt gcccgagaag gttctgacta acgaagacct ttcccgcatg
      481 gtggacacca gcgacgagtg gattaccact cgcaccggca tcaaggaacg tcgggtcgcg
      541 gcaaaggacg agtacacgag cgacatggcg gtgaaagcag ccttgaacgc gattgagcag
      601 gcgaagattt cgccgaagga aatcgatctc atccttttgg cgacggcaac gcccgacatg
      661 ctgttcccgg ccacagcctg cttcgttcag aaaaagatcg gcgcgacgaa cgccgcttgt
      721 ctggatattt ccgcggcgtg cgccggtttc attttcgggt tggaaatcgc ccagcagttc
      781 atcacgtcgc acacctacga caccgttctt gtgatcggag ccgacaaatt gacgtcaatt
      841 acgaattgga ccgatcgcaa cacctgtgtg ctgttcggcg atggtgctgg cgcggcgatt
      901 ttgcggcatc gtgggagtcc tcatggcatc atcaccaccc atattggaag cgacggccag
      961 tatacgaata tcctgttcat gccgggcggc ggatcgcggt gccccattac acgtgaaaac
     1021 gcggacatga acctgaccac tatccatatg accggcaagg aagtgtacaa acaggcggtc
     1081 atcgcgatgt tgaacgcagc caaaaaagca ctcgaacaag caggactttc ggtcgaggac
     1141 atcgcctgtg tgattccgca tcaggcaaac ctccggatta tggaggcgat cgccgatcgg
     1201 cttggcattt cgcgagataa aatgttcgta aacctcgatc gatacggcaa tacctccgca
     1261 gccgcggtag cgattgcgct cgatgaagcg aaccgcagcg gccgaatcaa accgggcgat
     1321 tacatcctga tggtcgtttt cggcggcggg ctgacgtggg ccagcacagt catcgagtgg
     1381 tgacgttgtc tgttgcctca gtcaggaatc tcgggctcga ccagcttcgc aagtaacgcc
     1441 aacgattcct gccagccgag gtaacaagct tcggcaggaa taacagcggg aagaccttcc
     1501 tgcaccacat tcagttcagt tccgcacgac accttcgtta acttgatgct cgtctgcatc
     1561 tcgcctggca gattcggatc atcgaatttg tcggtgtact tgatcctttc gtgcggagtc
     1621 aattcgatgt acgtcccgcc gaaggcatga cttttgccgg tcgtgaaatt cgtgaacgac
     1681 attttgtaag tgccaccaac tttggcgtct atctcgtgca cttttccggt gaatccattt
     1741 ggtggcagcc atttggccat cgcgtcagga tcgagaaacg ccttgtaaat cttttcaggc
     1801 gtcgctcgaa gaacgcggtg aagccggatt gtgtcggtct tggttgtcat gatgattgtc
     1861 tcttcagtta atttatcttt tcggtttcgg acgattcccg gaacgcct
//
LOCUS       DATMTI010000532         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_233619, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000532 DATMTI010000000
VERSION     DATMTI010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233619"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..380)
                     /locus_tag="VN039_08540"
     CDS             complement(201..380)
                     /locus_tag="VN039_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46051.1"
                     /translation="MLLLHNTFQHLGKGYGILAHAAPLEQAGPATGIAGGVLSFLAAS
                     GMSVVGIVWFLALGG"
     gene            669..1877
                     /locus_tag="VN039_08545"
     CDS             669..1877
                     /locus_tag="VN039_08545"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWV46052.1"
                     /translation="MATPFDSIENAIRDIKKGKCIILVDDEDRENEGDLVIAAEKVTP
                     HVINFMATHGRGLICLTLTPQRVEELQLPQQASENTATFGTAFTVSIDARKDITTGIS
                     AADRATTIHVAIDPKSKPSDLARPGHVFPLKAQTGGVLKRAGQTEGSVDLARLAGLCP
                     AGVICEIMNVDGTMARVPELTKFAKSHKLRMVTVKALIEYRMRRETFVRRMASARLPT
                     TFGEFEAVAFENQIDGVTHIALVKGPVDSGAPTLVRVHSGCLTADALGSLRCDCRDQL
                     HSAMEIIQKEGRGVLLYLNQEGRGIGLLNKIKAYGLQDQGSDTVEANLQLGFKADLRD
                     YGVGAQILANLGLHQIKLITNNPRKIVGIEGYGLKVVERVPIEIPPRAANVRYLRTKK
                     AKLGHLLKKV"
BASE COUNT          437 a          469 c          556 g          415 t
ORIGIN      
        1 ctcggcctca tctcactggg cgggttgggc gccgggagaa tgcgaaacct tcgcggacct
       61 caagcataaa acaaacatat cggggttcga ggagggggct tgatacctcg ttgagtatcc
      121 agcccgctct gtttgagcga cgagtaatcg tcgagcaatg ggtcaagcga tgtcagtggt
      181 gtacgcttcc ggatgatgcg tcaccctcct aatgcaagga accatactat ccccacgaca
      241 ctcatgccgg atgcggcgag gaacgagagc actcctcccg caattcccgt agccgggccg
      301 gcctgttcca gcggtgctgc gtgcgctaga atgccgtatc ccttccccag gtgctgaaag
      361 gtattgtgca ggagcagcat tgctcttgtt actacattca gaactctgcc gtttgacttg
      421 ctggtaggag tcacattgct gaaagcggat cgggaccgat tgtaaagtga tgaatgcgat
      481 aggataaggt atgtttcccg tggcacgttc cgaggtcggc gctgaggctt ccgatcggag
      541 gtcggtcgag ccgggccatt tttcgattga cagtcatgac agaatcaggg ttatgtgtca
      601 tcctcctaat tgctaaatct tcgatgttta atggtagatt gtcgtatcgt gaccgatagg
      661 acttagacat ggccactccg ttcgatagca tcgaaaatgc gattcgcgac atcaagaaag
      721 gaaagtgcat catcttggtg gatgatgagg atcgggaaaa tgagggtgac ctggtcattg
      781 ccgcggagaa agtcactccg cacgtgatca acttcatggc cacgcatggc cgaggcctga
      841 tttgtctgac tctgacgcct caacgggtgg aagaactaca gttgccacag caggcttccg
      901 agaataccgc gacgttcggg accgccttta ccgtctccat cgatgcacga aaagacatta
      961 cgacggggat ttcggccgcc gatcgggcca cgacgattca tgtggcgatc gaccctaaat
     1021 ctaagcccag tgatctcgcc agacccggtc acgtctttcc gctcaaggca cagacaggtg
     1081 gagtacttaa acgggccggg caaaccgaag gatctgtcga cctggctagg ttggcagggc
     1141 tatgtcctgc cggagtgatc tgcgagatca tgaatgtaga cggcactatg gcccgcgtac
     1201 ccgagctgac gaaattcgcc aagtcccata agctgaggat ggtgaccgtg aaggccttga
     1261 tcgaataccg catgcggagg gaaacctttg tcagacgcat ggcaagcgca cgattgccca
     1321 cgacatttgg agagtttgaa gccgtggcgt tcgagaatca gatcgacggg gtgacgcaca
     1381 ttgccttggt gaagggccct gtcgacagcg gagcgccgac actcgttcgt gtacactccg
     1441 gatgtttgac ggccgacgca ttaggatcgt tgcgttgcga ttgtcgcgat cagttgcact
     1501 ctgcgatgga gatcattcag aaggagggtc gaggggttct tttgtatctg aatcaagaag
     1561 gccgaggaat cgggttactg aataagatca aggcctatgg gttgcaggat caagggagtg
     1621 atacggttga ggccaatctg caactcggat tcaaggcgga tctccgcgac tatggagtcg
     1681 gcgcgcaaat tttggcgaat ctcggcctgc accagatcaa attgatcacg aataacccgc
     1741 ggaaaatcgt cgggatcgaa ggctatggtc ttaaggtcgt cgaacgtgtt cccattgaga
     1801 tccctccgcg cgccgcgaat gtccggtatc ttcggacaaa gaaggcgaag ctggggcacc
     1861 ttctcaagaa agtttga
//
LOCUS       DATMTI010000533         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_613874, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000533 DATMTI010000000
VERSION     DATMTI010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613874"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1450)
                     /locus_tag="VN039_08550"
     CDS             complement(227..1450)
                     /locus_tag="VN039_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV46053.1"
                     /translation="MITTSIRSRIIILAVIVCGLYLGYRMQEAKSDAALLHNKTLEEA
                     VPTVAVVSPKPLPGSETIVLPGNIVGWYEAPIYARVTGYVKMWHKDYGDQVKAGDLLA
                     EISTPDLDAEFQQAHADLESERVKYKLAEVTANRWIALRPNHAVSEQSITVQEQNMKA
                     QAAVVKAAEQKVRNIEAFIGFKKIIAPFDGIVIQRNINVGDLVSKEGNLNTPNAKTNL
                     FTVAVVDKLRLFVSVPEAFGPFLNPGLTADVTVPQLPHRHFNFQFLTVAKGFDIGTRT
                     ATTVFTIENEDRALWPGSYAQVHLTTPIDRQAFTMPSTALVFQERGTQVAVVTEDDHI
                     HLKPIKISKLMDQIVEVEEGLSAGDRIVNNPSAALLEGNKVRIVTPAVGYDLVTGEKP
                     APEAAAPKEGSSSPL"
     gene            complement(1447..>2841)
                     /locus_tag="VN039_08555"
     CDS             complement(1447..>2841)
                     /locus_tag="VN039_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV46054.1"
                     /translation="ATLVSSSIEELLPDQVENIVSNCGLPVGPHNLAFIPTPTVGSQD
                     CDLTILLKDEKSPVWEYRQIVRKGLKERFPGTEFTFQPSDLTAKILNFGAPAPIDVQV
                     NGPDIYASYEYARKLMDKYREISGATDVVIQQTMRTPTMMVEGDRTFGLGVNRTLSDM
                     ADNLLMTTAGSQQVDQVYWLDPSTGMSYLINVYTPQPFINSVNSLKTVPVDSSNDVTT
                     NADVQLLGNLTDLSVEGTPGVVTHGNIMPLFDIYVSAEGRDLGSVLGDVEKVTKSLEH
                     EKPRTAEIEIHGQAELMRDAYFELIFGLLAAIVLIYLLIVVNFQSWLDPFIIITALPG
                     ALAGIAWALFLTHTRLSEPALTGAIMCMGTGTANSILVVSYARERLQEHGNAILAAIE
                     AGTTRFRPVLMTAAAMIIGMVPMATGYSQNAPLGRAVIGGLLMATLFTLFFVPCVYAI
                     IYNRRTAAQQKEIS"
BASE COUNT          584 a          794 c          841 g          622 t
ORIGIN      
        1 ggtttcgcct ccacgaaggg gctggcccca tgccaggacg ccggcaccac atattgcggc
       61 ggctcatagg tcggcgccag attggcccgc ggcaaccaat ccccgcacgc cggcaaggtg
      121 agcgccagca acacccagcc gactcgccat gcgaagctgg accaatggta gggcattgcc
      181 tgttccgttc gcccgtgcgc acttctatcg gtcggccgta atgttgtcat aggggtgacg
      241 atgatccttc ctttggggca gcggcttctg gtgccggttt ctctcccgtg acaagatcat
      301 agccgacagc gggtgtgacg atgcgcacct tgttgccttc gagcagcgcg gcactcgggt
      361 tattaacgat acggtcgccg gcggaaagtc cctcttcaac ttcgacaatt tggtccatga
      421 gtttactgat cttaatgggc ttcaggtgga tgtggtcgtc ctctgttacc acagccactt
      481 gtgtgcctcg ctcctggaac accaacgcag tggagggcat cgtgaaggct tgtcgatcga
      541 ttggtgtcgt caggtggact tgggcgtacg agccgggcca cagcgcgcgg tcctcgttct
      601 cgatcgtgaa cacggtggtc gcggtgcgcg tgccgatatc gaaccccttg gcgacggtca
      661 gaaactggaa gttgaaatgc cgatgaggca attgcggcac ggtcacgtcc gccgtcaaac
      721 ccggattgag aaacggtccg aacgcctccg gcacgctgac aaagagacgc aacttatcga
      781 cgacggccac cgtaaacagg ttagttttcg cattgggagt gttaaggttg ccttccttgc
      841 tgaccaagtc gccgacattg atgttgcgct ggatgacgat cccgtcgaac ggcgcgatga
      901 tctttttaaa cccaatgaac gcctcgatgt tcctaacctt ttgctccgcg gctttcacca
      961 cggccgcctg ggctttcatg ttttgttcct gtaccgtgat cgactgttca gacaccgcgt
     1021 gattggggcg cagcgcgatc caacgattcg ccgtcacctc ggcgagttta tacttgacgc
     1081 gttcggattc gaggtccgca tgagcctgtt gaaattcggc atcgagatcc ggcgtgctga
     1141 tttcggcgag gaggtcgccg gccttcacct ggtccccgta gtccttgtgc cacatcttca
     1201 catagcccgt gaccctcgcg taaatcggag cttcgtacca accgacgata ttgccgggaa
     1261 gcacgatggt ctcggatcct ggaaggggct tgggagaaac gaccgcgacg gtgggcacgg
     1321 cctcttcgag cgtcttgttg tgcagcaagg cggcatcgct tttggcctct tgcatccgat
     1381 agccgagata gagcccacag acaatgacgg ctaaaattat gatacgtgat cgaatggatg
     1441 tcgttatcat gagatctcct tctgttgcgc agccgtacgc cggttgtaaa tgatggcata
     1501 cacgcagggc acgaaaaaca aggtgaagag cgtggccatg agcaagccgc cgatgacggc
     1561 gcgacccagc ggcgcattct gcgaataacc cgttgccatg gggaccatcc cgataatcat
     1621 ggccgcggcg gtcatgagca cgggacggaa gcgggtcgtc ccggcttcga tcgcggccag
     1681 gattgcgttg ccgtgctctt ggagtcgctc acgcgcatag gacaccacga ggatcgaatt
     1741 ggcggtccct gtgcccatgc acatgatggc gcccgtcagc gcgggttccg atagacgtgt
     1801 atgggtgagg aacagagccc aggcgatgcc cgccagtgcg ccgggtaagg cggtaatgat
     1861 aatgaaagga tcgagccagg attggaaatt gaccacgatc aatagataga tcaacacaat
     1921 cgcggcgagc agcccgaaga tcaactcgaa ataggcgtcc cgcatcagct cggcttgacc
     1981 gtggatttca atttcagcgg tgcggggctt ctcgtgctcc aggctcttgg taaccttttc
     2041 aacatccccc aggacggacc cgaggtcgcg tccctcggcc gagacataga tatcgaaaag
     2101 cggcatgata ttgccgtgcg tgaccacacc aggcgtaccc tccacggaca gatcggttaa
     2161 attgccgagg agttgcacat ctgcgttagt ggtaacatcg ttcgatgagt cgaccggaac
     2221 agttttgaga ctattgacac tattgatgaa gggttgcggc gtatagacat tgatcaaata
     2281 cgacataccg gttgagggat cgagccagta gacctggtcc acttgctggc tcccggcggt
     2341 cgtcatgagc agattgtcgg ccatgtccga cagagtccta ttgactccga gtccgaacgt
     2401 gcggtcgcct tcgaccatca tggtcggtgt gcgcatggtt tgctggatga ccacatccgt
     2461 ggccccggag atctcgcgat atttgtccat caatttccgg gcatactcat agctggcata
     2521 gatgtccggg ccgttcacct gcacgtcgat cggcgcgggc gcgccgaagt tgagaatttt
     2581 tgcggtcaga tcggacggct gaaacgtgaa ttcagtcccg ggaaatcgct ctttcaggcc
     2641 cttgcggacg atctgccgat attcccatac gggggatttt tcgtccttca aaaggatcgt
     2701 caaatcgcaa tcctgcgagc cgaccgtcgg cgtcggaatg aacgcgaggt tatgcggccc
     2761 gaccggcaga ccacaattgc tgacaatgtt ttcgacttga tcgggcaaca gctcttcgat
     2821 gctgctggac accaatgtgg c
//
LOCUS       DATMTI010000534         1693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_571608, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000534 DATMTI010000000
VERSION     DATMTI010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571608"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(569..1633)
                     /locus_tag="VN039_08560"
     CDS             complement(569..1633)
                     /locus_tag="VN039_08560"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWV46055.1"
                     /translation="MIGNRLKRAREAAGLSLRDLEAVINNEVSAQAIGKYERDEMMPS
                     SSVLLALAKALKVTAEYLLSTRELELCGVDFRKAPQAGVKEEKAVGAAVLDQVERYLE
                     LEELMPSIGPQWVELDKAEFMVDHIEAAERAADELRAMWKLGIDPIQNLAELLEDRGI
                     KVIALELPSNVSGSKAYVKISKHPDVPIIVVNGGHNGERQRFTMAHELAHLVLRPAGL
                     NDKDQEKAADRFAGAFLMAKEMLLKLLGGNRTSISLGEFVELKKIFKVSIACLVVRCS
                     QVGVISKALYGRLFGQIKSLGWNDLGSDEPGKLEPELPQRMERLCLRAISEGAISLSR
                     AAELLRISVRDLDLRLAQGA"
BASE COUNT          373 a          502 c          407 g          411 t
ORIGIN      
        1 aaattgtccg ctacagtaaa gcataatctg accgccactg aatccatagc cgtggctctg
       61 catctaagcc gttctaaccc catctgttaa gtcgagcgtt cacctcattt ttgggaaggc
      121 ggcagttttt atggtcagag atttgactca gtgcggcatg aagcgttgca ttcgtgaact
      181 tgtttgacgc ttcaatctga tcgagtgccc aaagtaggcc acaaaccctg gctccttgcg
      241 tttcagcttc ggtacgaaga gccccgtcgc ctgtcagcaa tatgtgatcg tcccggagcg
      301 cgcacaccaa ggcgaagcaa tctgccaaag agagtgtgtt gcgtcgggat ttcatcgtct
      361 gcaccaatga cagttcttct ggatttagcg ttagcacacc aagccctagt gctcgaaggt
      421 agggtccatt atgtggctca agctcgatct gatagagaac atccggcacc accagcacta
      481 acccacatgt gaacatgggc tccaagagtt gccctcgctc caggtcgatt agcactgagg
      541 tgtcggacac cagaatagcc atggcacgtt acgccccctg cgccagccta aggtcgagat
      601 cccgaacact aatcctgagc agctccgccg ccctcgatag cgaaattgcg ccctccgaaa
      661 tggcgcgtaa acagagacgc tccattcgtt gtggaagctc gggctccaac ttgcctggct
      721 catcagaacc caggtcgttc catccaagtg acttgatctg cccaaacaat cggccgtata
      781 gagccttgga tatcactccc acttgtgagc aacgtacgac gaggcacgca atactgacct
      841 taaaaatttt cttgagctct acgaattctc ctagtgagat agacgtacgg ttccccccga
      901 gcagcttcag caacatctct ttagccatga gaaacgcgcc agcgaatcga tcagcagcct
      961 tttcctgatc tttgtcgttg aggccagccg ggcgcaaaac caaatgggct agttcgtgcg
     1021 ccatcgtgaa tctttggcgc tcgccgttgt ggccaccgtt gacgacgatg atggggacat
     1081 cggggtgctt gctgatcttg acgtaagcct tggatcccga gacgttgctc ggcagttcca
     1141 gggcgatcac tttgatgccc ctgtcttcca gtagctctgc caaattctgg atcgggtcaa
     1201 tgcccagttt ccacatggct cgcagctcat ccgcagcacg ctcggctgct tcgatgtggt
     1261 cgaccatgaa ttccgcttta tcaagctcaa cccactgagg tccgatgctc ggcatcagct
     1321 cctccagttc cagatagcgc tcgacttgat caagaaccgc cgctcccacc gccttctctt
     1381 ctttgacacc cgcttggggc gccttgcgga agtccacccc gcagagttct aactcgcgcg
     1441 tactaagcaa gtactcagcg gtcaccttca gcgccttggc caaggccaag agtaccgacg
     1501 aactgggcat catctcatca cgttcatact taccgattgc ctgtgccgaa acctcattgt
     1561 ttatgactgc ctcgaggtct cggagtgaga gccctgcggc ttctctcgcc ctcttcagtc
     1621 tattgccaat catcttgtcc tcctcgttac gtggtttaca tattgacagt aaaaagtatc
     1681 gtttgtaaac ttc
//
LOCUS       DATMTI010000535         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_827025, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000535 DATMTI010000000
VERSION     DATMTI010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827025"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1866
                     /locus_tag="VN039_08565"
     CDS             148..1866
                     /locus_tag="VN039_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWV46056.1"
                     /translation="MPNSFPRGRRYVLLLLLLCGFGVVLFRLVTLQVLQAAELSVKAD
                     RQHQKTVSLEGARGTIVDRHGKLLAKNVEVPSVFGVPNALHSPLTTARQLSPILHMKS
                     DELERKLRQDRSFVWLARKLDPEQGRQLDRLSLDGIGVVMEGRRFYPKGSLLAHVLGF
                     SGMDGEGLEGVEHRYESYLRGEKRMMVLQRDALGRTVFPRGMTERSPTPGQSLTLTID
                     EVIQYLAERELDDAVGRAQAKSGTMIVLDPKTGAVLAMAVSPRFDPNAVSALSPDRWR
                     NRALTDAYEPGSTMKAVMAAAAIEERVVRPNTMVFGEHGHMTVANTVIHDHERLGWVS
                     FAQVIQKSSNIGAAKTGMALGEQRLHRYLLAFGFGQRTEIDLPGEGAGLVRNPRDWGR
                     RSVASISMGQEIGVTPLQMVSAVAAIANGGVLMKPYVVSEIRDADGHILRQTPPQARR
                     RVISPETARSVTRILEGVITDGTGTKAAIRGFRVAGKTGTAQKIDPRTGTYSASRFVA
                     SFAGYVPADNPQLAMIVVIDEPQGDAWGGAVAAPVFSRVGEQVLSYLGVPSYEPVTLA
                     MASRKS"
     gene            1885..>2541
                     /locus_tag="VN039_08570"
     CDS             1885..>2541
                     /locus_tag="VN039_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWV46057.1"
                     /translation="MTLATLLQVLEGQVRILDRHGNLDVSINAITDDSRAVSTDSLFV
                     AVKGEQVDGHEFIPAAVRGGMGALVSQQPVSGVSLPFVRVDDSRKALGLLGSRFYGDP
                     SSHIRMIGVTGTNGKTTTTYVCKTLLEALGHPVGLIGTVVYQIGERTMPATHTTPGSL
                     ELQQLLATMVAGRCTTTVMEVSSHALAQDRTSGCEYDVAVFSNLTQDHLDFHKTMEEY
                     F"
BASE COUNT          568 a          618 c          779 g          576 t
ORIGIN      
        1 attagcgacc gaaaaactcg gcatgaatct gcctcaacag gggcaggtca ttatagttca
       61 gtccaggcca agttatgtct atccttcaaa tgttgtgccg attgagctga aaatcgccaa
      121 gaattatccc gccagcagga ggctctagtg ccgaattcct ttccgcgcgg tcgtcggtat
      181 gtcctgttgc tgctgttgct atgtgggttt ggagtggttc tgttccgatt ggtcacattg
      241 caagtgttgc aagcggcgga actttcggtc aaagccgatc gacagcatca aaagacagta
      301 tcgctggaag gggcgcgcgg cacgattgtc gaccgacacg gcaagcttct ggccaagaat
      361 gtggaagtgc cgtccgtgtt cggagtccca aacgcattac acagtcccct caccaccgct
      421 cgacagttgt cgccgattct gcatatgaag agcgatgagt tggaacgaaa acttcgacag
      481 gaccggagtt tcgtgtggtt ggctcgaaag ttggatcctg agcaggggcg acagcttgat
      541 cggttgtcgc ttgatggaat cggggttgtg atggagggga ggcggttcta tcctaaaggg
      601 tcactcctgg ctcatgtgct gggtttttcg ggaatggacg gtgaaggtct ggaaggcgta
      661 gagcatcgct acgaatcgta cttgcgtggt gaaaagcgga tgatggtgct gcagcgtgac
      721 gccctaggac gtacagtgtt tcccagggga atgactgaac ggagtccgac acccggccaa
      781 agtctcacgc ttacgatcga tgaggtgatt cagtatcttg ctgaaagaga gcttgatgac
      841 gccgttggcc gtgcgcaggc aaagtcagga acaatgattg tgcttgatcc aaagacggga
      901 gcggtgttgg ctatggcggt gagtccgcga tttgacccta atgccgtgtc ggctctcagc
      961 cctgatcgct ggcgaaacag ggcacttacg gatgcctatg agcccgggtc caccatgaag
     1021 gcggtgatgg ccgcggcagc cattgaagaa cgagtggtaa ggccgaatac gatggtattc
     1081 ggcgagcatg gtcatatgac ggtcgcaaac accgtgattc atgatcatga gcgactgggc
     1141 tgggtgtcct ttgctcaggt gatacaaaag tccagcaaca tcggggcggc gaaaaccggt
     1201 atggcgctcg gagagcaacg actccatcga tatctcctgg cattcggttt cggacagcga
     1261 acggaaatcg accttcctgg ggaaggggcc ggattggtga gaaatccaag agactggggg
     1321 cgtcgttcgg ttgcctccat ttccatgggg caagagatcg gtgtgacgcc tcttcaaatg
     1381 gtatctgcag tagcggccat cgcaaatgga ggtgtgttga tgaaacctta cgtggtttca
     1441 gaaatacgag atgctgatgg ccacatcctt aggcagacac cccctcaagc caggcgacgg
     1501 gtcatttctc cggaaacggc ccgcagcgtg acgaggattc ttgaaggtgt catcacggac
     1561 ggaaccggaa ccaaggccgc tattcgggga ttccgagtgg ctggaaaaac cggtacggcc
     1621 caaaagatcg acccacgaac agggacctat tcggcatctc gatttgttgc gtctttcgca
     1681 gggtacgtgc ctgcagacaa cccgcagctt gccatgatcg tggttatcga tgagccccaa
     1741 ggtgatgcgt gggggggagc tgtcgccgcc cccgtgttta gtcgtgtcgg ggaacaggtg
     1801 ttaagctact taggcgtgcc gtcgtatgag cctgtcacct tggcaatggc ttcgcggaaa
     1861 tcgtgatcct atttggacat gtcgatgact ttggcaaccc tgcttcaggt tctcgaagga
     1921 caggtgagga ttcttgatcg tcatggaaat ttggacgtgt ccataaatgc gattaccgat
     1981 gattctcgag ccgtttcgac cgacagtctc tttgtagccg taaaaggtga gcaggtcgat
     2041 ggacatgaat ttattccggc ggcagtgagg ggaggcatgg gcgcattggt gtcgcaacag
     2101 ccggtgagcg gggtgtctct tccattcgtc cgcgttgacg actccagaaa ggcactcggt
     2161 ttgctgggga gccgttttta cggagacccc tcgtcgcata ttcgaatgat cggcgtgact
     2221 gggacgaacg gaaaaaccac caccacatat gtctgcaaaa cgcttctgga agccctgggc
     2281 caccccgtag ggttgattgg aacagtcgtc tatcagatcg gtgaacgcac gatgccggca
     2341 acgcacacga caccaggttc tcttgaactg caacagctgc ttgccacgat ggtcgccggt
     2401 aggtgcacga ccacagtgat ggaagtatcc tctcatgccc tggcgcagga tcgcacgagc
     2461 ggatgcgaat atgacgtggc ggtcttttcg aatctgaccc aggatcatct cgattttcac
     2521 aagaccatgg aggagtattt t
//
LOCUS       DATMTI010000536         1537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_360329, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000536 DATMTI010000000
VERSION     DATMTI010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360329"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..134
                     /gene="tatA"
                     /locus_tag="VN039_08575"
                     /pseudo
     CDS             9..134
                     /gene="tatA"
                     /locus_tag="VN039_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(927..1445)
                     /locus_tag="VN039_08580"
     CDS             complement(927..1445)
                     /locus_tag="VN039_08580"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV46058.1"
                     /translation="MTFPVVSSASHQGKSLAVDPGRETINVHDTSGQLLGSMSWGAII
                     ERVLVSGEDARFAHCRTLPRAPLALKVRYTTPEGKQFDSLTGGIGGGGLFIESGAPLS
                     PGTELTVEFALPDRPWDKLKAKAKVAWVRNKPERYLLFPGMGIQFVELDKKVQEQLVG
                     LVEALNRSRNPS"
BASE COUNT          363 a          476 c          377 g          321 t
ORIGIN      
        1 ggccgtacat gttcggcaca atgggtttct ccgaactcat tatcattttg gtcatcgtgc
       61 tgattatttt cggagcaggc cgtctgccgc agataggcga aggtgtcggc aaagcattga
      121 aagggtttag aaaagaagtc aacgaagggc cccaatccgc tccaggtcag gaccagcaac
      181 aaaccgagca gccacaagcc attcacattc caacgagcat cgcagtgaca ccgtctctga
      241 ccgcaggatc atcgccagca acggccaggc caaacactcc ccatgtccct ggtccggaac
      301 tcacgcccgg caccaccgcc tccttgttgt acaatacaag ccttcagact tcgcgagcat
      361 cggctccttc cccaaatcaa gcatcgacag ctgcctccaa cccggtgcta tcgatggagg
      421 aacgggctgc cgctccccct ccacgggctc atgcctcgta tccacctttg ccgaccggct
      481 cccaagcaaa agcgacggcc aaacgtcctt cagccatcgt caataaggac gcggttgccc
      541 gtgtgcaagc acaacaagct gccctcaaag ctaagcaggc accatcagtg gttggagtat
      601 cgcccgatga tctgcagcat ctcggtgaag gtctcggaga agtcctgcga acgttccggc
      661 aaaccgtgac cgatgttcgc aatagcgtgg atcctcaaat gtgtacgatt cagggcgaaa
      721 tggaggcggc caaaaaggaa gtccagcaat ccattgaagc agctaacgca ctaccaccgg
      781 tgcaagagga gcctccgaaa tctgcgtgat tctcatatca acggctctcc gcatttctct
      841 caaatccttg ccgaccgatg tgtgcccgct tcgcaccact gccatacagg ctttgttgtc
      901 gctataggta agcgagccta ttctgatcac gaggggtttc gacttcgatt caaagcctca
      961 accagcccga ccaattgctc ctgaaccttc ttatccaact ccacgaactg aatgcccatg
     1021 cctggaaaca aaagatatcg ctccggcttg ttgcggaccc aggcgacctt cgccttggct
     1081 ttcaacttgt cccacggtcg atccggaagc gcaaattcga ccgtcagctc ggtgcctgga
     1141 ctgagaggag caccactctc gataaagagc ccgccgccgc cgattcctcc ggttaggcta
     1201 tcaaactgct ttccttctgg cgtcgtatat cggaccttca gggccagcgg cgcacggggc
     1261 agcgtacgac aatgggcaaa tcgggcatct tcaccgctga caagtactcg ctcgataatt
     1321 gcgccccatg acatgctgcc taaaagctgg cccgaggtgt cgtgaacgtt gatcgtctct
     1381 cgcccaggat caacagcgag agatttcccc tgatgactcg cactggaaac gacgggaaac
     1441 gtcataaaac catccctttc ccttttgggg cgggcccaag tcatgaacgg aaactaggct
     1501 gcttgactca tgttctggat tgttttcact aactcgt
//
LOCUS       DATMTI010000537         1590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_317682, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000537 DATMTI010000000
VERSION     DATMTI010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317682"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1590)
                     /locus_tag="VN039_08585"
     CDS             complement(<1..>1590)
                     /locus_tag="VN039_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWV46059.1"
                     /translation="PIRVEFLGDQVESIRLFDASTQTSIEKLKDGWVLPAREFVRPPD
                     DPDAIMSIPADAEWRSPDLYTSMDTLFDYLTAAPFLALDQPDALRKACEAAWEKIDDG
                     YLRHVDRDATHPYPSPERLFLTWPGIQQRIETWPMLALEPLAAPDSTWNQTFSFSAQA
                     PGSIGLGIRGTAFSQTLSLLEGLRNEHRVVLVARSRGQVDRLLALLREHDLPADPWKP
                     TVWSGHRTGKLPFYVLHGDLSAGFLSGDLRLALLTEEELFAKGARHKPQPKSRTATFL
                     SSLEDLNVGDYVVHVQHGIAKYRGLKRLSIQDYESDFLILEFSGGDTLYVPLDRLNQV
                     QRYSGAESHVPRLDRLGGTSWAKTTARVKKDIEEMAHELIDLYANRELVKRNAYGTTT
                     TLYHEFEAAFEYEETVDQLRAIEDIGRDMESTKPMDRLVCGDVGYGKTEVAMRAAFKA
                     VEHDRQVAVLVPTTLLGHQHYENFAERFAPFPMRVALLSRFQSPRETKTILKDVAAGT
                     VDIVIGTHRLLQKDVEFRQLGL"
BASE COUNT          314 a          448 c          482 g          346 t
ORIGIN      
        1 gaggccgagt tgccggaatt ccacgtcttt ctggagcagc cggtgcgtgc cgatcacgat
       61 atcgacggtt ccggccgcca catctttgag aatcgttttg gtttccctgg gagattgaaa
      121 ccgtgagagc agcgcgacgc gcatcggaaa gggcgcgaat cgttcggcaa agttctcata
      181 gtgttgatgg ccgagcagag tggtcgggac cagcaccgcc acttggcggt catgctccac
      241 ggctttgaag gcggcgcgca tggccacttc cgtctttccg tatccgacgt cgccgcagac
      301 gagtcggtcc atgggctttg tcgactccat gtcgcggccg atgtcctcga tggccctgag
      361 ctgatccacc gtttcttcat actcaaaggc tgcttcgaat tcgtgataga gagtcgtagt
      421 cgtgccatac gcattccgtt tcaccagctc ccggtttgcg tagagatcga tcaattcatg
      481 ggccatctcc tcgatatcct ttttcacccg cgcggtagtc ttggcccaac tggtgccacc
      541 caagcgatcg agacgcggga catggctttc ggctccgctg taccgttgga cttggttcaa
      601 ccgatcgaga ggaacgtaaa gcgtatcacc accggagaac tcgagaatca ggaagtcgct
      661 ttcgtaatct tggatcgaga gtcgcttgag ccctcgatat ttggcgatac cgtgctgcac
      721 gtgcacgacg tagtcaccga cgttgaggtc ttccaaggaa gagaggaagg tcgccgttct
      781 gcttttcggc tgcggtttat ggcgggcacc cttcgcgaat aactcttctt ccgtcaggag
      841 ggcaagccga aggtctccgg agaggaaccc cgccgagaga tcgccatgca gaacataaaa
      901 cggcaacttg ccggtcctgt ggccggacca gaccgtcggt ttccatgggt cggcaggcaa
      961 atcgtgctct ctgaggaggg cgagcaaccg gtcgacctgc ccccggcttc gtgctaccaa
     1021 gacaacccga tgttcgtttc ggagtccttc taacaggctc agtgtttggc tgaaggcggt
     1081 gcccctgatc ccgagtccga tactcccagg tgcttgggcg gaaaacgaaa acgtctgatt
     1141 ccaggtcgag tccggcgccg ccagcggctc aagggccagc atgggccacg tctcgatccg
     1201 ctgttggatg cccggccagg tcaggaagag gcgttcgggc gagggatagg ggtgcgtggc
     1261 atcacggtct acatgacgga gatagccgtc gtcgatcttt tcccaggccg cctcacatgc
     1321 ttttcttagc gcatccggtt gatcgagggc gaggaaggga gcggcggtga gatagtcgaa
     1381 cagtgtgtcc atggatgtat acaggtcggg gcttcgccac tcagcatccg ctgggatcga
     1441 catgattgca tccgggtcat ctggagggcg aacgaactcc cgcgcgggca acacccagcc
     1501 atctttcaat ttttcgatgg aggtctgggt cgaagcgtcg aacaaacgaa tcgattcgac
     1561 ctggtctccg agaaattcca cacggatcgg
//
LOCUS       DATMTI010000538         1626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_190666, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000538 DATMTI010000000
VERSION     DATMTI010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190666"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VN039_08590"
     CDS             complement(<1..1434)
                     /locus_tag="VN039_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV46060.1"
                     /translation="MKNMEIAAPPFCVEVDYPKPSREYVKKKYMADFDKRVLALSAVL
                     DGASIQEAAKAHGVDRTTLSRMIEAVAKIAPDGKPFGYRVCIPWYRVTDTEVLEAEVP
                     LASGPGAFTQLLVALPELKALVSSYRGKLPSHNDPSPKFDLFFRRFVANLKGKGLECL
                     YPLNCWDSGRRALQNWIKRERKALLDAQVAQNEDVDLTLTRVDQLFKLQPLDRVEIDG
                     HIIDVEWHAQVPTKDGKWTAEQISGIWILVGIDVVSLAAFPCTLVIGAAYDRYDLLDA
                     FANTLRPWAPRDLIVPKLQYAFGAWMPPMADNAADVHRPASVAVDNALAHKAKMATEN
                     FGDHQLGVINLGFPHVPEGRPHIEAFFKRLEEAVLRLLAGGFRPAKERGAKKRKVSTK
                     SAKNHPVNIEALRDLMDVEISSYNVTPHPALQNRTPREVFEDHINDDMWATQSFLTAE
                     DAAELLVLHLTVTIRGDKRKGVLPYVKF"
     gene            complement(1431..>1626)
                     /locus_tag="VN039_08595"
     CDS             complement(1431..>1626)
                     /locus_tag="VN039_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46061.1"
                     /translation="LRARILDVAKRFDRVRISQIIGALDGFPPGDIRCVIADLIHAGQ
                     LSIDPKSQLTGHSIVQVRRA"
BASE COUNT          318 a          446 c          492 g          370 t
ORIGIN      
        1 aaatttcacg taagggagca cgcccttgcg cttgtcacct cggatggtca cagtcaggtg
       61 aagcacgagc agttcggcgg cgtcctcagc agtcaggaag gactgggtcg cccacatgtc
      121 gtcgttgata tggtcctcga agacctcccg cggggtgcgg ttttgcagcg ccgggtgcgg
      181 cgtcacgttg tacgacgaaa tttcgacatc catgaggtcc cggagcgcct caatattcac
      241 tgggtggttc ttggcggatt tggtggatac ctttctcttt ttggcaccgc gctccttcgc
      301 tggacgaaaa cccccagcta aaagcctcag taccgcttct tccagacgct tgaaaaaggc
      361 ctcgatgtgg ggacgtcctt cgggtacgtg tgggaatcca aggttgatga ctcccaactg
      421 atggtcaccg aagttttcgg tagccatctt ggctttgtgg gcgagggcgt tgtccacggc
      481 aaccgacgca gggcgatgca cgtcggccgc gttgtcggcc atggggggca tccatgctcc
      541 aaacgcgtat tggagtttgg ggacgataag gtccctcggc gcccaaggac gcagcgtgtt
      601 cgcgaatgca tcgagcaggt cgtaacggtc ataggcggcc ccgatgacca acgtgcatgg
      661 gaatgcggcc agcgagacaa cgtcgatccc taccaagatc catatgccgc ttatctgttc
      721 ggcggtccac ttgccatctt ttgtcgggac ttgagcatgc cactcgacgt caatgatgtg
      781 cccgtctatc tcgacgcgat caagtggctg cagtttgaaa agttgatcga cgcgggtcag
      841 cgtaagatca acgtcttcgt tttgtgcgac ttgagcgtcg agtagcgcct tacgctctcg
      901 cttgatccag ttctggagtg cccggcggcc actgtcccag cagttcagcg ggtagagaca
      961 ttcgagccct ttgcctttca gattcgcaac gaatctgcgg aagaagagat caaacttcgg
     1021 gctgggatcg ttatgcgatg ggagcttgcc cctatagctg cttacgagcg ccttgagttc
     1081 cggtagggcg acgagtaatt gagtaaatgc cccagggccc gatgccagag gaacctcggc
     1141 ttcgagcact tcagtatcgg tcactcggta ccacgggatg cacacccggt agccgaatgg
     1201 tttgccgtct ggggcgatct tcgcgacggc ttcgatcatg cgcgacaacg ttgtgcgatc
     1261 gacgccgtgt gctttagctg cctcttgaat ggacgctccg tcgaggaccg cgcttaaggc
     1321 gagcacacgt ttgtcgaaat cggccatgta ctttttcttg acgtattccc ggcttggctt
     1381 cgggtagtcg acctcaacgc agaagggtgg ggcagcgatt tccatgtttt tcatgcgcgc
     1441 ctcacttgga caatcgagtg tcctgtcaac tgcgacttcg ggtcgatcga cagctggccg
     1501 gcatgaatca agtcggcaat cacgcagcgg atgtcaccgg gtgggaaccc atcgagcgca
     1561 ccgatgattt gcgagatccg cacacgatca aagcgtttag cgacgtcgag gatgcgcgcg
     1621 cgaagg
//
LOCUS       DATMTI010000539         8640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_360374, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000539 DATMTI010000000
VERSION     DATMTI010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360374"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VN039_08600"
     CDS             <1..381
                     /locus_tag="VN039_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV46062.1"
                     /translation="VPEYHPSPDDTFENAVLLFDQHAVEFLYVLDNEGILQGVITRNE
                     LFEAFAQGKKPSTKVRDFMRNDPIAVTSKDTSLMASDLMNKHDIDRLPVVESKDNRRL
                     IGLVRSEKMLRWLVEQSHVDPPGS"
     gene            536..1159
                     /locus_tag="VN039_08605"
     CDS             536..1159
                     /locus_tag="VN039_08605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWV46063.1"
                     /translation="MLRSSDKIHIVIINGSVRPGNYTNMASALVLDELKKHTKVSTDV
                     IDPAALRLPPPGTDPQSKDAKQLQQKVGQATGIILATPEYYGSFSSVMKLVIESLGYP
                     SALSGKPVALLGVAGVTIGAVKSLEHLRSVVSHVGGIVLPLPISVANVRSVFDDEGKC
                     LDAEAEQLVRSVGTNLLTYIEHNVCPGVMLERLLSEGEKLPPKIVGI"
     gene            1356..2261
                     /locus_tag="VN039_08610"
     CDS             1356..2261
                     /locus_tag="VN039_08610"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV46064.1"
                     /translation="MESIGFIGLGKMGSAMDGNLLKAGFTLRVYNRTPEKASALGAQG
                     ARLVRQPGDAAESGGIVITMLANDRALEDIVGGDGGFVERLGPGGIHLSMSTVSPRLA
                     RRLAEQHRRYGVEYVAAPVFGRPEAAAARKLWICLSGSAMAKQRVRPILDSIGQGIFD
                     FGEEPVAAHVVKLAGNFLLASAIEALAEAFVLGEKNGIDRNSLADMLGSTLFACPAYQ
                     TYGRAIAQQRFRPAGFTVELGLKDIDLVLQTGADSTTPLPLAGLLHDRFLAAVAKGRG
                     ELDWSSVWLESAENAGLSVEYTHEE"
     gene            2468..3643
                     /locus_tag="VN039_08615"
     CDS             2468..3643
                     /locus_tag="VN039_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV46065.1"
                     /translation="MRLAIGVLFVVGAVGLFGCKGETASSAPRPIPEVQVVEVTSQTI
                     PDEPELIGQAEASSIVEIRPQVTGIIKQRFFPEGRDVKQGDRLYEIDPVPFRAAFVSA
                     KGRVAQAEARVVQAKQDLARVKPLLVEDAVSQKDVDDAVAEDLAARASLEAARGDLVK
                     AKFDLDNTLIVAPVDGLIERTRYYQGRLVTAQTDLLTVIHQVDPMYVIVSAPESFLLK
                     RRRDTLSKRIQHPGIYKLTGTIIFVDGTTYAHEGVLDFADIGLQVQTGSRQARVVFPN
                     PDRVLLPGQFVTVRFHGVSKPNAILVPQRAVQQGPKGAVVYVVSQGNQVEVRDVKATD
                     WQGDWWIIEEGLRAGERVMVEGFQRVVPGAQVKPVTVASMASNIPPSSDVGDAEQAK"
     gene            3640..6822
                     /locus_tag="VN039_08620"
     CDS             3640..6822
                     /locus_tag="VN039_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWV46066.1"
                     /translation="MNLGFFIDRPIFASVLSIVVVVMGLVAMLALPIAQFPEITPPVV
                     QIEADYPGASAEVVAESVARPIELQLPGIDHLLYFDSTSTNDGHMTIKLTFEIGTNVD
                     IAQVQAQNRQKLAEPQLPPEVIRQGVTVKKMSPDLLTVMVLNSDDPQQDALFLSNFAI
                     LRILDNIKRLPGVGDALVFGQQNYSMRLVLDPVRMAQLGLTPTDIANVVREQNRDFPA
                     GTVGREPMPISTELTLPLFADGRMSDVHEFENMIVRALPNGSMVRLKDVARVELGAQS
                     YVLEGRYNRKPTALLLTFLLPGANALDTVKGIRGEMDRLSKIFPPGVTYDIPYDTTRF
                     VDVSIKEVVKTLGEAMVLVVLVVYLFLQSWRATLIPVLAVPVSLVGTFAGMAALGFSI
                     NTLTLFGLVLAIGIVVDDAIVVVENVERHMEGGLSPKDAAKKAMGEVAGPVIAIVLVL
                     CAVFVPVGFLGGITGQLYKQFAITIAVSVAISGFVALTLSPALCALVLKPSHGLRKGF
                     FGLFNRLFEWVQIRYSTSVGFTLRHTVLSLALCGIVIGVAVKLFAGIPLAFLPEEDQG
                     YFITVVQLPDGASKKRTDAILDRIEQYYHTLPAIYGTQALSGQNFVFNTRGTNTATLF
                     APLKHWDERKSKQDHVKSLIGGAFKEFAKIPGALILAFNAPSIRGLGATGGFSVQLQD
                     PSGGDFRQFGAVAQEFVEKARQDPAIGAVSTSFRVSAPRVHAKINRERAKALGVPISE
                     VFDTLQAYFGNLYINDFVKFGRVYRVQTEAEPQYRSRPADISKIYVRALNGRGSTMIP
                     LDTVVSTTYSSGPDPVTHFNGFNTALVLGSAAPGYSSGQALDALERVAKEILTPQGFD
                     IDWSGISYQEHKAGSQSVVAFGFGLLMVFLVLAAQYESWTVPFAVILAVPFGVFGALS
                     AVWLSGMTNDIYFQIGLVTLIGLSAKNAILIVEFANQRYQHGLPLRDAASEAARLRFR
                     PILMTSLAFILGVVPLVAAGGAGAASRHSIGTGVFGGMIAATILSVLFVPLFFMLIRM
                     LGKPATGPIQRDRIGEDRTGLESVSPQGGR"
     gene            6824..8257
                     /locus_tag="VN039_08625"
     CDS             6824..8257
                     /locus_tag="VN039_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWV46067.1"
                     /translation="MRISAVIGLSWTLFACAVGPDYSRPAIPAGDSFRMAKEASDLPS
                     LANMPWWELYQDEELQKLIRIALEENKDLERAVATIEEFEARLFIARTDFIPQMTGTG
                     NFPFARSGGVRFRGFSNPFNYYLQGNLAWEIDVWGRIRRSNQAARGDLLAREENRRAI
                     VLQLVGGVAQAYFELRQLDLQSEIAERTLKAWDESVRISQARHRQGLIHGLDVEQFQA
                     ERENAAARIADLKRQMIQKENELSVLLGRNPGQIPRGQPLTDQVVPPIVPAGLPSELL
                     QRRPDIVQVEQQLAAATARIGAAKADRFPKLSLTGLLGIANPSLSRLFLPGGDFGIFG
                     PTLTGPLLNAVSLGFQQDAAEAQARQAVAQYKQTILVAFKEVEDALAGVTMTREQAAA
                     QERQVNALKAALRLANLRYKGGLANYLDVLIAQRNLFDAELALATTRRLYLTSVVQLY
                     KALGGGWSLSDHSREQPVTEALKVGNN"
     gene            8374..>8640
                     /locus_tag="VN039_08630"
     CDS             8374..>8640
                     /locus_tag="VN039_08630"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HWV46068.1"
                     /translation="MNAGCAAHCTRCGAELFRSRSFHIDTVLALTLGSLVVFVMANAF
                     PIVTLEMKGVHNGSTLFGAIVTLYREGMAIVSGLVLATTILFPLT"
BASE COUNT         1771 a         2427 c         2614 g         1828 t
ORIGIN      
        1 gttccagagt atcatccgtc cccagacgat acgtttgaaa atgccgtcct cctgtttgat
       61 cagcacgcgg tcgagttcct ctacgtcctc gacaacgagg gaatcttaca aggggtcatc
      121 acgagaaacg agttgttcga agcctttgcg caagggaaga aaccgtcaac caaggtgcgg
      181 gatttcatgc gtaatgatcc cattgcggtt acgtcgaagg atacgagttt gatggcgagc
      241 gacttgatga acaagcacga catcgaccgg cttcccgtgg tcgagagcaa ggacaatcga
      301 cgtctgatcg ggctcgttcg gtcggagaag atgctgcgct ggctggtgga acaatcccat
      361 gtcgacccgc ctggttctta atgcgtcgag gcaacctggc tcaggcgatg accgatcata
      421 aaacgggcag gatggggcac ctcgattgaa caggtggctg tctcgtgcgg gtgatgcggg
      481 tgttcaaaca gacggacgag acaagtggaa ataattctgg tgtcaggggg aacctatgtt
      541 gcgctcatcc gacaagatcc acatcgttat catcaacggc agcgtacgtc ctggcaacta
      601 cacgaacatg gcgtctgcac tggtcctgga cgaactgaag aagcacacca aggtctcgac
      661 cgatgtcatc gaccctgccg cgttgaggct tccaccacct gggacggacc cacagtccaa
      721 ggatgcgaag cagctccaac agaaagtggg acaggccacg gggatcattc ttgccacgcc
      781 ggaatattac ggcagcttca gcagcgtgat gaagctcgtc atcgaaagtc ttggctatcc
      841 gtctgccctg tccgggaagc cggtcgcgtt actgggtgtc gcaggtgtaa cgatcggtgc
      901 cgtgaaatcg ttagagcatc tgcgcagcgt ggtttcgcat gtcgggggca tcgtgttgcc
      961 gttaccgatt tcagtcgcga atgtccgcag cgtcttcgac gacgagggca aatgtctgga
     1021 tgctgaggcg gagcagctgg tccgcagcgt cggcacaaac cttctcacct acatcgagca
     1081 caatgtctgt cccggagtga tgctggagcg gctgctcagc gagggagaaa aactaccacc
     1141 aaagatcgtc gggatttagg cgatctacca tgatccgtct gaacacaaat ggaaactcgc
     1201 cttggccaaa gcggggaagg gatctgtgga ggtattttta ccgcgagaga cacggcggca
     1261 tggttgtgag cgacgagatc atcattaaga tcaacgccga gtgtagcaag gtttaggcga
     1321 atacatagta gagcggtaac cgaggagttc atgcgatgga atcgatcgga ttcatcggac
     1381 ttggcaagat gggcagcgct atggacggaa acctgctcaa agcgggcttc acgcttcggg
     1441 tctacaaccg caccccggag aaagcctccg ccttgggggc gcaaggggca agacttgtcc
     1501 gtcagcccgg cgacgcggcc gagtccggcg gtattgtgat caccatgttg gcgaacgatc
     1561 gggcgctgga ggacatcgtc ggtggcgacg gagggtttgt ggaacgcctc ggtcccggcg
     1621 gcatccatct ttcaatgagc acggtctccc cgcgtctggc acgccgctta gccgaacagc
     1681 accgacgtta cggggtcgag tacgtcgctg ccccggtgtt cgggcgcccg gaggcggcgg
     1741 ccgcacggaa gctatggatc tgcctgtccg gctccgcaat ggcgaagcag cgggtacgcc
     1801 ctatcctcga ttccatcggg caaggcatct ttgactttgg cgaggaaccg gttgcggcgc
     1861 atgtcgtcaa gttggcgggg aactttctgc tggcctcagc cattgaggct cttgccgaag
     1921 cgtttgtgct cggagaaaaa aacggcatcg atcgaaacag tttggcggat atgctgggca
     1981 gcactctgtt tgcctgccct gcctaccaga cctatggccg cgcgatcgcg cagcagcgtt
     2041 ttcgaccggc aggcttcacc gtggagttgg gattgaagga tatcgatctg gtgcttcaga
     2101 ccggagccga tagcacgacc ccgcttccat tggccggctt gctccatgat cgattccttg
     2161 ccgccgtcgc aaaagggcga ggggaacttg attggtcttc cgtctggctg gagtccgcag
     2221 agaatgcggg gttatcggtc gaatatacgc acgaggagtg agtcgtccag cgatcagaat
     2281 ctccggtagc gacacaactg aaaccgtaaa ggaagggtct catgaggatc gctgttacga
     2341 cgcctcaaga cattcttgga ctggaaagat cacagaggta tatacccttg acatctcata
     2401 cctaccgcgt agatcctcac tgcgtgagtt atgtcatgaa aactgttcag cgaacgaacc
     2461 gatgccgatg agacttgcca ttggcgtgct tttcgtcgtt ggagcagtcg gcctgttcgg
     2521 ctgcaagggc gaaaccgcct cctccgcgcc tcgtccgatc cccgaagtcc aagtcgtcga
     2581 agtcacctct cagactattc cggatgaacc ggagcttatt gggcaagccg aggcgtcgag
     2641 catcgttgag attcgtccgc aagtcaccgg aatcatcaag caacggtttt tccccgaggg
     2701 gcgcgacgtc aagcagggcg atcgtctcta cgagatcgat cctgtaccct tccgggcggc
     2761 attcgtgagc gcgaaaggac gagtcgccca ggccgaggcg cgggtcgttc aggccaagca
     2821 agatctggcg cgtgtcaagc cgttgctcgt cgaagatgcc gtcagccaga aagatgtcga
     2881 tgacgctgtc gcagaagatc ttgccgcgcg ggcctcgctg gaggcggcaa gaggagatct
     2941 ggtcaaggcg aagttcgatc tggataacac gctcatcgtc gccccggtcg acggcttgat
     3001 cgagcggacg cggtactacc agggccggtt ggttacggcg cagacggatc tcctgacggt
     3061 catccatcaa gtcgatccta tgtacgtgat cgtgagcgcg ccggagagtt ttctcctcaa
     3121 acgacggcgg gacacccttt cgaaacggat tcagcacccg gggatctaca agttgacggg
     3181 caccatcatc ttcgtagacg gaaccaccta tgcgcatgag ggcgtgttgg attttgcgga
     3241 tatcgggttg caggtccaga ccggttcacg gcaagcgcgg gtggtgttcc cgaaccccga
     3301 tcgggtattg ttacccggcc aattcgtgac cgtgcgtttt cacggagtgt cgaagcccaa
     3361 tgccatcctt gttccccagc gcgcggtcca gcaaggacca aagggagccg tcgtctatgt
     3421 cgtcagccag gggaaccaag tggaagtccg agacgtgaag gcgacggatt ggcaaggaga
     3481 ttggtggatc atcgaggaag ggcttcgggc cggggagcgg gtgatggtgg aaggattcca
     3541 acgagtcgta cccggagctc aggtcaagcc cgtcacggtt gcctcaatgg catccaatat
     3601 acctccatcg tcggacgtcg gtgatgcgga acaagcgaaa tgaacctcgg tttttttatc
     3661 gaccgtccga tttttgcctc ggtcctttcc atcgtcgtcg tcgtcatggg cctcgtggcg
     3721 atgctggccc tgcccatcgc tcaattccct gaaatcactc cccctgtggt gcagatcgaa
     3781 gccgactatc ccggcgcgag cgcggaagtc gttgcggaat cggtcgcccg ccccatcgag
     3841 ttgcagcttc ccgggatcga ccatcttctc tatttcgatt cgaccagtac gaacgacggt
     3901 cacatgacca tcaagctaac cttcgaaatc ggcacgaacg tcgatatcgc gcaagtccag
     3961 gcacagaatc gccagaagct tgcagagccg cagcttccgc cggaagtcat tcgtcaagga
     4021 gtgaccgtta aaaaaatgtc gccggatctg ctgacggtca tggtgctgaa ttccgacgat
     4081 ccgcagcaag atgcgctctt tctctcgaat ttcgcgatcc tgcgaatcct cgacaatatc
     4141 aagcgcttgc ccggcgtcgg cgatgcgctg gtcttcggac aacaaaacta cagcatgcgt
     4201 ctcgtcctcg atccagtgcg catggcgcag ttgggtctga caccgaccga catcgcgaat
     4261 gtcgtgcgcg aacagaaccg tgactttccc gccggaaccg tcggacggga gcccatgccg
     4321 atttccacgg aactcaccct cccgctcttt gccgacggac gcatgtccga cgtgcacgaa
     4381 ttcgagaaca tgatcgttcg agctctgccg aacggatcga tggttcgtct caaagatgtc
     4441 gcgcgggtcg agttgggcgc gcagtcttac gtgctcgaag gccgctataa ccgcaaaccc
     4501 accgcgctgc ttctcacctt tcttttgccc ggcgccaacg ccctagacac agtcaaggga
     4561 attcgcggtg agatggacag gctgagcaag atctttccac ccggcgtcac gtatgacatt
     4621 ccctacgaca cgacacgctt tgtcgatgtc tccatcaagg aagtggtcaa gaccttgggg
     4681 gaagccatgg tcttggttgt cttggtggtg tatctctttc ttcaaagctg gcgcgcaaca
     4741 ctgattcccg tcctagcggt tccggtctca ctggtcggca cctttgcagg gatggcggcc
     4801 ctcgggttct cgatcaatac actcaccctc ttcggcctgg tgctcgccat cgggattgtg
     4861 gtggacgatg ccattgtggt cgtggaaaac gtggagcgcc acatggaggg agggctgtcc
     4921 ccgaaggacg cggccaagaa ggccatgggg gaggtggccg gccctgtgat cgccattgtg
     4981 ctggtgctgt gcgcggtctt tgtgccggtg ggatttctcg gaggcatcac cggccaattg
     5041 tacaagcagt ttgcgatcac gatcgcggtc tctgtcgcga tctcggggtt tgtcgcactg
     5101 acgctcagtc cggcgctctg cgccctggtg ctgaagccga gccatggact acgcaaaggc
     5161 ttctttggac tgtttaaccg gctgttcgag tgggtccaaa ttcgatactc gacaagcgtg
     5221 ggtttcacgt tgagacacac ggtgctctca ctggcattgt gcgggatcgt gatcggcgtc
     5281 gcggtcaaat tgtttgccgg gattcccttg gccttcctgc cggaggaaga ccagggatat
     5341 tttattaccg tcgtccagct gccggatggg gcgtcgaaaa aacggacgga cgcaatcctc
     5401 gaccggattg agcagtacta ccataccctg cccgcgatct atggaacgca agcgctgtcg
     5461 ggacagaact ttgtcttcaa taccagagga accaatacag cgaccctctt cgcgccgctc
     5521 aagcattggg atgaacggaa gagcaagcag gaccatgtca aatctcttat cggaggggcg
     5581 ttcaaggagt ttgcgaagat ccccggtgcg ctgatcctgg cgtttaatgc tccctcgatc
     5641 cgaggcctgg gcgccaccgg cggtttttcc gtgcaactgc aagacccgag cggcggagat
     5701 tttaggcagt tcggcgcggt cgctcaggag ttcgtcgaga aggcaagaca ggacccggcg
     5761 atcggggcgg tcagtacaag ttttcgcgtg agcgctcccc gcgtgcacgc caagatcaat
     5821 cgagagaggg ccaaggcgct cggggtgccg atctccgaag tgttcgacac ccttcaggcc
     5881 tatttcggca acttgtacat caacgatttc gtgaaattcg gtcgtgtcta tcgggtccag
     5941 accgaagcgg agccccagta tcgatcgcgc cccgccgata tcagcaagat ctacgttcgg
     6001 gcattgaatg gccgaggctc aacgatgatc cccctggaca cagtcgtctc gacgacctat
     6061 tccagcgggc ccgatcccgt cacccatttc aatggattca ataccgccct tgtcctgggt
     6121 tcagcagcgc cgggctacag ttcaggtcag gcgctcgatg cattggagcg ggttgcgaaa
     6181 gagatcttga cccctcaggg gttcgatatc gattggagcg ggatttccta tcaggaacac
     6241 aaggcgggat ctcagtcggt tgtcgcgttc gggttcggct tgctcatggt gttcctggta
     6301 ctggccgccc agtatgagag ctggactgtt ccgtttgccg tgatcttggc cgtgccgttc
     6361 ggcgtgttcg gcgccttgtc ggcggtgtgg ctatcgggga tgaccaacga catttatttc
     6421 cagatcggcc tcgtcacgct catcgggctt tcggccaaga acgccattct catcgtggag
     6481 tttgcgaatc aacgatatca gcacggcctc ccgttgcggg atgcggcgag cgaggcggcg
     6541 cggcttcggt tccgccccat cctcatgacc tcattggcgt ttattctcgg cgtggtgcct
     6601 ttggtcgccg ccggcggagc aggcgcggcc agccggcatt cgatcgggac cggtgtgttc
     6661 ggaggaatga tcgccgcgac gattctttcc gtcttgtttg tccccttgtt cttcatgctg
     6721 atccgaatgt tgggaaagcc ggctaccgga cctatccaac gagacagaat cggagaagac
     6781 cgtaccggac tcgagtcggt gtccccgcaa ggaggacgtt gacatgcgca tttcagctgt
     6841 gatcggactt tcgtggacac tgttcgcctg cgcggtgggg cccgattatt cgcgccccgc
     6901 tattcctgcc ggagattcgt tccgcatggc caaggaagcg tccgacttgc cgtctctcgc
     6961 caatatgccg tggtgggaac tctaccagga tgaagaactt caaaagctca tccgtattgc
     7021 gttggaagag aacaaagatc tcgaacgcgc agtcgcgacg atcgaagaat ttgaagctcg
     7081 gttgttcatc gcgaggaccg acttcatccc ccagatgacg ggcaccggga actttccttt
     7141 cgcaaggtcg ggcggtgtcc gttttcgagg attctccaac cccttcaact actacctgca
     7201 ggggaacctc gcctgggaaa tcgacgtgtg gggacgaatc cgccggtcga accaagcggc
     7261 gcgcggcgac ctgctggctc gggaagagaa ccggcgcgcg attgtcttgc aattggtcgg
     7321 tggagtggcg caggcctatt tcgagcttcg gcagcttgat ctgcaatcgg agatcgccga
     7381 acgcacgttg aaagcatggg atgaatccgt acgaatcagc caagcacgcc accggcaagg
     7441 attgattcac ggattggatg ttgaacagtt ccaagccgag cgggaaaacg cggcggctcg
     7501 catcgccgat ctgaaacgcc aaatgattca gaaagaaaac gagttgagcg tgctgttagg
     7561 gaggaatccc ggtcagatcc ccagaggaca accgctgacc gaccaagtgg tgccgccgat
     7621 tgtgccggcc ggtctccctt ctgaattgct gcagcgacgg ccggatatcg tgcaggttga
     7681 gcagcagctg gctgccgcga cagccaggat cggggccgcc aaagccgatc gctttcccaa
     7741 actctccctc accgggcttc tcggcatcgc caatccaagc ttgtccagac tcttcttgcc
     7801 cggaggcgat ttcgggatct tcggtccgac cctgaccggt cctctgctga atgccgtgag
     7861 tctcgggttt caacaggatg ccgctgaagc tcaagccagg caagcggtcg cccaatacaa
     7921 gcagacgatc ctcgtggcat tcaaagaagt tgaagacgcg ctggcgggag tcacgatgac
     7981 acgtgagcag gcggctgctc aggagagaca ggtcaatgct ctcaaagcgg ctctgcgtct
     8041 ggcgaatcta cggtacaagg gcggccttgc gaactatttg gacgtcctga ttgcgcaacg
     8101 gaatctgttc gatgcggaat tggcgctcgc gacgactcgc cgcctgtacc tgacatcggt
     8161 ggtccagctg tacaaagcct tgggcggcgg atggtcgctc agcgaccaca gtcgggaaca
     8221 gccggttacc gaagctttga aagtcggaaa taactagcgc cgcgggcgcc ggatggccgg
     8281 tgccgagacc atctctgtcc aacccctctt agggacatga cgcaccgact gattgcttgc
     8341 gaagaatgcg atgtcctcca ccgtcggata gttttgaacg ccggatgtgc cgcgcactgt
     8401 acccgatgcg gcgcggagtt gttccgctct cgatcattcc acatcgatac ggttctggcg
     8461 ttgacactcg gcagcctcgt ggtgtttgtg atggcgaacg cattccccat cgtgaccctc
     8521 gaaatgaaag gagtgcacaa tgggtcgacg ctctttgggg cgattgtgac gctctaccgc
     8581 gaaggaatgg ctatcgtatc cgggctggta ttggcgacga cgatcctctt cccgctgacg
//
LOCUS       DATMTI010000540         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_571978, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000540 DATMTI010000000
VERSION     DATMTI010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571978"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VN039_08635"
     CDS             <1..368
                     /locus_tag="VN039_08635"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWV46069.1"
                     /translation="CDLWFITNEDSPKSVEIHSNEEVLVSIQSKRDEFVTLSGRAEVV
                     HDPQKVAELWRESFKVWFPQGVNDPSIALIHVAGRHGEYWDNSGLNKASFMLESFRAF
                     FTGTQPDVKEGSQHGKLSI"
     gene            542..1426
                     /locus_tag="VN039_08640"
     CDS             542..1426
                     /locus_tag="VN039_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWV46070.1"
                     /translation="MIAEHRRAEELVGRLDVLLNRLVSHWDPAGPELAEVQAVYAMLA
                     QDLHRHYALEEKALFPVLSQYRTMMLMEVEHDDLLALQQAFEDQLERFPLGQTATQAQ
                     AQEQTVEALETLRQRFEAYKTRLLAHMVEEERGIFPLANERLEPEEKLKVLRLTNELN
                     ESKRPAAYDLIRPEPGFILKKANLSASSNKPMDYQTLFEREHSVIQTLRLHAGQKQAF
                     HWAGQTQVMVVLLGELKFEARCGIGQTDGNDEIKAQALSVGDTVTLDSRLVFSLSAMT
                     EALLMVFKLWPHPHYTKS"
BASE COUNT          335 a          383 c          464 g          324 t
ORIGIN      
        1 actgcgacct ttggtttatc accaacgaag acagccccaa gagcgttgaa attcactcca
       61 acgaagaggt gctggtcagc attcagagca agcgggatga attcgtgacc ctgagcggac
      121 gcgccgaagt ggtgcacgat ccgcaaaaag tggccgagct ttggcgggaa tcctttaagg
      181 tctggttccc gcaaggggtg aatgatccca gcatcgcgct catccacgtg gcaggccgcc
      241 atggggagta ttgggataac tccgggctga acaaggcctc gttcatgctg gaatccttcc
      301 gggccttctt cacgggaact cagcccgatg tgaaggaagg ctcgcaacac gggaaactca
      361 gcatataggg gctcgctcgt gccctcatcc acaccaggaa tgaaaggcaa cccccaaggg
      421 gtaacctgga atgcttgcta ctgaaaagga tcgggccgat cgattcttta gctttttggt
      481 tttgcgtgaa aatcctgggt tgtccgggta agataggggc atgacggact gttgtgaact
      541 gttgattgcc gagcatcgcc gcgccgagga attggtgggg cggctggatg tgctgttgaa
      601 ccggctggtg agccattggg atcctgccgg gccggaactg gccgaagtcc aggcggtgta
      661 tgccatgctg gcccaggatc tgcatcgaca ttacgccttg gaggagaagg cgctattccc
      721 ggtgttaagc cagtaccgca ccatgatgct gatggaagtg gagcatgatg atttgctggc
      781 cttgcagcag gcttttgagg atcaactgga gcgttttccc ttggggcaga ctgccactca
      841 agcgcaagcc caagagcaaa ctgttgaagc attagaaacc ctccggcaac ggtttgaggc
      901 ctataagacc cgtctgctgg cccatatggt ggaagaggaa cggggcatct ttccgctggc
      961 caacgagcgc ctggagccgg aggaaaagct gaaggtgctt cggctcacca acgagctgaa
     1021 cgagtcgaaa cgaccggcgg cttatgactt aatccgcccg gagccgggct ttatcctgaa
     1081 aaaggccaac ctgtcggctt ccagtaataa gcccatggat taccagaccc tgtttgagcg
     1141 ggaacatagc gtcattcaga cccttcgcct ccatgcggga cagaagcagg ccttccactg
     1201 ggcaggccag acgcaggtca tggtcgtttt gctgggagag ctgaaatttg aagcccgctg
     1261 cggtattggg cagaccgatg ggaatgacga aatcaaagcg caggcgctca gcgtgggtga
     1321 tacggtgacg ctagattccc ggctggtgtt ctctctgtcg gccatgacgg aggccttgct
     1381 gatggtgttc aagctatggc cgcatccgca ttatacgaag tcttaaagca aagcggcctt
     1441 aagctggttt taaacgggct tgagccggtg gcctgacagt ggggtttgtt tctcttgtat
     1501 tgcctc
//
LOCUS       DATMTI010000541         1840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_274554, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000541 DATMTI010000000
VERSION     DATMTI010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274554"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VN039_08645"
     CDS             complement(<1..558)
                     /locus_tag="VN039_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HWV46071.1"
                     /translation="MKVAINLLWVLLAVLGAFALAHVTGAANPHEKVNGLWLVVAAAC
                     IYVLAYRFYGRWIARQVVGLNNQHVTPAVRLNDGVNFHPTNKVVLFGHHFAAIAGAGP
                     LLGPVLAAQFGFMPGFLWLVIGAVLAGAVQDFIILVASMRRNGRSLPEIAHDELGSTT
                     GTATAVAVLFIVVVALAGLGFAVVNA"
     gene            868..1530
                     /locus_tag="VN039_08650"
     CDS             868..1530
                     /locus_tag="VN039_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWV46072.1"
                     /translation="MLTLVQLPWSPFCITIRHILTRHRIPFRLHNIAYHDRAPIIKAT
                     KGQGHTVPCLIDGRHAICDLTDFGQEVAHYVDRRYRLNLFPKNLDGLQLILSRYIEND
                     VESVGFKVNDTYVIPIRPLIERTMLIRHKERKFGRGCLREWTTHRRYLCTQFVELLKP
                     IDDMLAASLFLLTDRPLFVDYNLYGVLGNYLFNGKTRLPSLKHLRRWYQVMDTRNYPD
                     LS"
     gene            complement(1565..>1840)
                     /locus_tag="VN039_08655"
     CDS             complement(1565..>1840)
                     /locus_tag="VN039_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46073.1"
                     /translation="GLKEHLAWKEQGAGENFVHREGLVECNADTPAADVDRPLDERFL
                     RLVALKLKADRQGDGDAIKLAPICRRRLRSWLVRWHGAEEYSKKQDE"
BASE COUNT          427 a          542 c          458 g          413 t
ORIGIN      
        1 agcattcacg acggcgaacc ccaaacccgc caacgcaacc acgacaataa agaggaccgc
       61 caccgctgtt gccgtgccgg tggtcgagcc cagttcatcg tgcgcaatct cagggagcga
      121 tcgcccattg cgtctcatgg aagccaccag gatgatgaaa tcctgcaccg ctccggccag
      181 caccgcccca attaccagcc agagaaagcc gggcatgaat ccgaattgcg cagccaacac
      241 cggcccgagc aagggtccgg cccctgcaat tgccgcgaag tgatggccga agagcacgac
      301 tttattcgta ggatggaaat tgacgccgtc attcagccgc accgccggtg tcacatgctg
      361 gttgttcagt ccgacgactt gtctggcgat ccatcggcca tagaaccgat aggccagcac
      421 atagatgcag gccgccgcca cgacgagcca aagaccgttt actttttcgt gtggattagc
      481 ggcgcccgta acgtgagcaa gcgcgaaggc cccgaggacc gcgagcagca cccagagcag
      541 attaatcgcc actttcatgc gcggcatcct aacacaaaaa gaaagcatgt agcctgcttg
      601 caggagtaac caaaagtaag gaattgttgt gggtatgcgc ggcctattga attaggattg
      661 cccgcattac ttttgatcca actggcctaa gccatgtttc ggcagtaagt gagatggcag
      721 aagatcagga accagcagct gatttggatc accggcggac tcagatgatc ctactggcct
      781 tgctcgtacg tttttcctga gcaccagcag ccattaacac acccatcgac gcttgcggtt
      841 tcgcttcgct tccttcatat aagctccatg ctcacgctcg ttcagcttcc ttggtccccc
      901 ttctgcatca cgatcaggca catcctgacg cgccatcgta ttccgtttcg gcttcacaat
      961 atcgcctatc atgatcgagc tccgatcatc aaagcgacaa aaggtcaggg tcacacggtg
     1021 ccctgcctga tcgacgggag gcacgccatc tgtgatttaa cggacttcgg acaggaggtt
     1081 gcgcattatg tggatcggcg ttaccggctt aacctctttc ccaaaaacct cgatgggcta
     1141 caactaattc tttcgcgcta tatcgagaac gatgtggagt cggtgggatt caaggtgaac
     1201 gatacgtatg tgattccaat ccgtccactc atcgaacgga cgatgctcat tcgccacaag
     1261 gagcggaaat tcggaagagg atgcctccga gaatggacca ctcaccgacg gtatctgtgc
     1321 actcagtttg tggaactctt gaagccgatc gacgatatgt tagcggcttc gctgtttctg
     1381 ctgaccgatc gcccattgtt cgtcgattac aatctatacg gcgtactcgg caattacctg
     1441 ttcaacggaa agacccgatt gcccagtctg aagcacctcc gacgctggta tcaggtgatg
     1501 gatacgagga actatcctga tttgtcttaa gacatctacg tacgcttttc aattacggct
     1561 cattctattc atcctgcttt ttgctatact cttccgcacc atgccatcgg acaagccagc
     1621 ttcgcagccg tcgcctgcag atcggcgcga gttttatcgc atcaccgtca ccttgcctat
     1681 ctgccttcag cttgagagcg accaggcgga ggaaacgctc atccagcggt ctgtcaacat
     1741 cagcggcggg ggtatcggcg ttacactcaa ccagaccttc tcggtgaacg aaattctctc
     1801 ctgcaccctg ctccttccag gccaggtgct ctttaagtcc
//
LOCUS       DATMTI010000542         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_530192, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000542 DATMTI010000000
VERSION     DATMTI010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530192"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..833)
                     /locus_tag="VN039_08660"
     CDS             complement(57..833)
                     /locus_tag="VN039_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV46074.1"
                     /translation="MEERFSFLDPHGHRVAAVLTVPNGETDKISILCHGFLSSKTSST
                     NNALTRMLIGQGIAAFRFDFFGQGESEGPFDQITVSLAVEQTQRAVELMRDRGYRHIG
                     LMGSSFGGLVSILTASQRTDLACLALKCPVVDFAEELRLEFGDVGMALWKATGTIPNI
                     MGGPDRITLHYAFYEDALRQIAYVPARSITAPTVIVQGDEDEHVPLHQSRQLYEALLV
                     KKHLEMLPGANHQFTKVADFTLMTQLIADWLTQHLSSAQS"
     gene            complement(893..2218)
                     /locus_tag="VN039_08665"
     CDS             complement(893..2218)
                     /locus_tag="VN039_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46075.1"
                     /translation="MFARTIPPVIFCITGFSWLVLASILGMATLIGLVHGTPLPSWVR
                     ALHVHATLLGGVAQIILGGFILMIPPPNSANRKEADSHPLAFWAMNSGLIGMLVGFWL
                     HRDLVVSVSGFVVIAAFLSVIYTVWIRASRTWKFSFKESWYYALSLFCLVAGSAFGEI
                     MAFGLIPESHGYVRLAHIHLVVIGFVVLAIIGMVHHILPTVWNRPLVSDTLARTGTIV
                     MPIGMAVLIGGFLNSSVPIELAGGAILFLGGILWTGSLFRTWLFSSHTGSAASDQLFV
                     SAFFLIFTVILGILVGANNLSSPSVLPYGKLHLVAYTHMAFVGFVMNAVMGAFSYLLP
                     IALAADRISSHKKRGAYLDRLTTIMNRWRTVQIATLNLGTMGLGILAALTWNVPLTSM
                     SIRVASWTSLSLLMTGLVLFSVKLTSIIAKKPDQLQTGQISTDELKLTA"
     gene            complement(2228..3028)
                     /gene="thiD"
                     /locus_tag="VN039_08670"
     CDS             complement(2228..3028)
                     /gene="thiD"
                     /locus_tag="VN039_08670"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWV46076.1"
                     /translation="MSNTLKQVLTIAGSDSGGGAGIQADIKAMSANGVFAMSVITAIT
                     AQNTEEVTDVLELPPAIIASQLDAIFDDFDVAAIKTGMLSSSAIVEVVVKLMKPQNVV
                     NLVVDPVMISKSGHSLLRPDAIEAVKTKLLPLALVVTPNVHEAQQLSGIEIASLADAR
                     RAAKVIHGFGCKHVLIKGGHLINERATDLLYDGRFFNVLKGEFIETRHTHGTGCTFAS
                     ALAAHLARGRSVLDSAQAAKSYVTEAIRHGLAIGHGQGPTDHFYFLER"
BASE COUNT          792 a          879 c         1001 g          719 t
ORIGIN      
        1 gtctaagata cgatgtgcag tgtcggccga accgtggatc gcttcaggca ggtcgattat
       61 gactgagcgc tcgacagatg ctgtgtcagc caatcggcga tgagctgtgt catcagtgta
      121 aagtcagcta ccttcgtaaa ctgatggttc gctcctggta gcatctcaag gtgtttcttg
      181 accagcagtg cttcgtagag ctgtcggctt tggtgaagcg ggacatgttc gtcctcgtcg
      241 ccttgcacaa tgacggtcgg agccgtgatc gatcgtgcgg ggacataggc aatctgccga
      301 agagcatctt cgtaaaaggc atagtgaagc gtgattcggt cggggccgcc catgatgttc
      361 ggaatcgtgc ccgttgcctt ccatagtgcc attccaacat ccccgaattc gagccgtagc
      421 tcctcggcga agtcgacgac cggacatttc aaggcgaggc aggccaagtc tgtccgttgc
      481 gaagcagtca aaatcgagac cagaccgccg aaacttgatc ccatcagtcc gatgtgccga
      541 tacccgcgat ctctcatgag ctcgaccgct ctctgtgttt gttcaaccgc caagctgacc
      601 gtgatctgat cgaacgggcc ctcgctttcc ccttggccga agaagtcgaa gcgaaaagcg
      661 gcgatgcctt gaccgatcag catgcgggtc agtgcattgt tcgtcgagct ggttttcgat
      721 gaaagaaacc cgtgacacag gatggaaatt ttgtccgttt ctccgttggg gacggtcaaa
      781 acggcggcta cccgatggcc gtgaggatcg agaaatgaaa accgttcttc cacggaagga
      841 ttgtatcaga gtcggtgtct gaggtcagga tgggagtcga acccgtcgga agtcaggccg
      901 tgagcttgag ttcgtccgtg gagatctgtc ctgtctgaag ctggtccggt ttcttggcga
      961 taatggaggt cagcttgacc gaaaagagaa ccaagccggt catgaggagg ctaaggctgg
     1021 tccagcttgc aacacggatg gacattgagg tcagcggcac attccaggtc agtgccgcca
     1081 agatgccgag tcccatggtt cctaggttga gggtggcgat ttgaacggtc ctccatcggt
     1141 tcatgatggt cgtgagccgg tcaagatagg ccccccgttt cttatgacta gaaatgcggt
     1201 cggctgcgag ggcgatcgga agtaggtagg agaatgcgcc catgacggca ttcatgacga
     1261 agccgacgaa tgccatatgc gtgtaggcta caaggtggag tttgccatac ggcaataccg
     1321 aggggctgga gagattattt gctcccacaa ggatgcctag aatgacggta aagatgagaa
     1381 agaaagcgct gacgaagagt tggtccgaag cggcgctgcc ggtatggctt gagaagagcc
     1441 atgttcgaaa caggctgccg gtccagagga tcccgcccag gaaaagtatg gcgccgccgg
     1501 ccagttcaat cgggacggat gagttcaaga atccaccgat taagacggcc atgcctatcg
     1561 gcatcacgat tgttcctgtc cgggcaagcg tgtcgctcac tagtggacgg ttccagaccg
     1621 tcggcaagat atgatgcacc atcccgatga tcgccaagac cacaaagccg atcaccacca
     1681 ggtgaatgtg cgcgagccgc acgtagccat gggattccgg gatgagcccg aatgccatga
     1741 tttctccgaa ggccgatccg gcgacgaggc agaagaggga aagcgcatag taccaagatt
     1801 ctttgaatga gaacttccaa gtcctgctcg cacggatcca aacggtgtag atgacggaaa
     1861 gaaacgcggc aatcaccacg aagccggaca cacttacgac taggtccctg tgcaaccaga
     1921 accccacgag catgccaatc aagccgctgt tcatcgccca aaatgcgagg ggatgggagt
     1981 ccgcttcttt tcggtttgcc gaattcggcg gagggatcat caagatgaat ccgccgagaa
     2041 tgatttgggc tacaccaccg agcaaggttg catggacatg gagcgctctg acccacgacg
     2101 gcaagggggt gccatgaacc agcccgatta gggttgccat gcccagaatc gacgccagca
     2161 ccagccaact gaaacccgtg atgcaaaata tgacgggggg aatggttcgt gcgaacatag
     2221 gtatgtgcta tcgctctaag aaataaaaat ggtccgtcgg gccttgcccg tgcccgatcg
     2281 ctaagccatg tcggatcgct tccgtgacat aggacttggc ggcttgcgca gaatctaaga
     2341 cggatctccc tcgtgcaaga tgagccgcca atgcggaggc gaacgtacag ccggtgccat
     2401 gagtatggcg ggtctcgatg aactctccct tcaacacatt gaaaaaacgt ccatcataga
     2461 gcagatcggt cgctcgttcg ttgatcaggt gcccgccttt gatcagaaca tgcttgcatc
     2521 cgaagccatg aatcactttg gctgctcgcc gggcatcggc caacgaggct atttcaatcc
     2581 ccgacagctg ctgcgcctca tgtacgttcg gtgtcacgac caaggcaagt gggagcaatt
     2641 tcgtcttgac ggcctcgatg gcgtccggcc gcaagaggga atgaccgctc ttggagatca
     2701 tcacggggtc cacaaccaga ttcacgacgt tttgaggttt catcaacttc acaaccacct
     2761 cgacgatggc cgatgaggac aacattccgg tcttgatggc ggcaacgtcg aagtcgtcaa
     2821 agattgcatc gagctgcgat gcaataatgg ccggcggtaa ttcaaggaca tccgtcactt
     2881 cttcggtatt ctgagccgtg atcgccgtga tgacggacat ggcgaacaca ccgttggcgg
     2941 acatggcttt aatgtccgcc tgaatgcctg cgcctccacc ggaatccgag ccggcgatcg
     3001 tgagcacctg tttcaatgta ttcgacatga atgatccttt acggagtggt ctgcgcaggt
     3061 tccgacggcg agccgatctt cagccaatcg ggctcctagt agtagcccgt cagccaattg
     3121 cttgaggctc tcctcatcac ttgccccggc acatgatgtc ggatgtgaga aggatgttgc
     3181 gaaaccgacg agcagtacca ggagtccgat catacgcatg aactgatgtc attatactca
     3241 gggaaggtct gctgtctagc gggccgtaaa aaatggtgcc gcgaaacagg tgttgaaggg
     3301 gcatggaata ggatggtcgc cgtctggttg agctttgacg tggactgctg ggatgcggtc
     3361 tttgtcccgg gaattggacg gctggaatat g
//
LOCUS       DATMTI010000543         4574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_104876, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000543 DATMTI010000000
VERSION     DATMTI010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104876"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..945)
                     /locus_tag="VN039_08675"
     CDS             complement(397..945)
                     /locus_tag="VN039_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46077.1"
                     /translation="MRVLDFDIETRRIGFHNGGRFNPDGCEVVILAAAWDGEPVQTWE
                     LRSRWTEKDARAIVSAFRTLYDEADMVTGHYIRKFDLPILNGACLEWGLPPLGRKLVL
                     DTKTDLIDVAGLSQSQENLSALKDLDASKFHMHDNNWRKVARLTPEGLALARERVVDD
                     VLQHQELRGELTGWLAPTVWNP"
     gene            complement(1462..1779)
                     /locus_tag="VN039_08680"
     CDS             complement(1462..1779)
                     /locus_tag="VN039_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWV46078.1"
                     /translation="MLLNKVGYSRKRVRYLSEGYRSLRHMKDTKPGRPLSLLVELVDL
                     DIAQQYLTDDEYVTWLMCGICGYTTREAAEFLGVSHMTVSRRYDRAIEVLKSVMNNDD
                     QAM"
     gene            complement(1766..2128)
                     /locus_tag="VN039_08685"
     CDS             complement(1766..2128)
                     /locus_tag="VN039_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HWV46079.1"
                     /translation="MPDAIASEAEAVAEHLPRVAHLARRYSGLEGAEFDDLYQEGSIT
                     VLLAVREERRPSNLEIERDMFNWIAKCRRWRRQESMSEDMLDESGIHSDPGRRKEHWT
                     DSNDVPQGGWSESFYAPK"
     gene            complement(2128..2418)
                     /locus_tag="VN039_08690"
     CDS             complement(2128..2418)
                     /locus_tag="VN039_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46080.1"
                     /translation="MNGKQVTDVVIRTAGSQKLVSITIWPEFKLTAEPKKGDFIAADG
                     SFSTYSYQAQDGSQRENLQLNPIHLVHLPCVEKEDTRQVVQSGVSGGSGTPF"
     gene            complement(2668..3024)
                     /locus_tag="VN039_08695"
     CDS             complement(2668..3024)
                     /locus_tag="VN039_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46081.1"
                     /translation="MADKPWKAWERKVAKDLGGTRTGPRGHDLPDSIDAPLIAPECKL
                     YEKFIFLEADMEQARNNAKNIPGNKIPAVFIKEKKRGGRAAVRMDYADFLRLHDLAML
                     GRWELEAASREVNESR"
     gene            complement(3014..3352)
                     /locus_tag="VN039_08700"
     CDS             complement(3014..3352)
                     /locus_tag="VN039_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46082.1"
                     /translation="MRKSGPDTDWRDKVSRALGDEEEEQQAIAYAPQHPSDLEIAFHI
                     DAGKMWVPHAVLIDSGYSLLEDGSIVFVNNRFYELQGYSYSRKMWWVEEFVVDGTFDD
                     ITPEDIINGG"
     gene            complement(3466..>4574)
                     /locus_tag="VN039_08705"
     CDS             complement(3466..>4574)
                     /locus_tag="VN039_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV46083.1"
                     /translation="SKTTVFLVEGETDTMRLWQELWDNNSSAGTTRGVVGLSGIETWR
                     DELAAAFTGQRVYVILDNDRDYNVQARVDTAWRNIRASLGGKARRVRLPGDVKDICEF
                     FDAGYILDHLFRAAESGTGQSRWHPLDLTQAPPPVPWLVENMIVRGATHTITGEPGIG
                     KSWLTMALCLGVIRGTDWLGQKVDAPGRVLYADEENAEVLIFDRLRRKLGMTDEEARQ
                     LRYLSNQGILLDRDPDALVDEALEFEPALIVLDSLTRFHMQDEDKAGPMARLYNMALK
                     PLARETGAALVVLHHAGKTESTSSYRRSRGSGEIVANVDVGIDARQSGINSMTLATFK
                     SRFRQAGDVLHVAIVDDHEGGVSLIGGVNPEPPF"
BASE COUNT          859 a         1434 c         1221 g         1060 t
ORIGIN      
        1 tcacttttct ctagacccct tgacagaagt tctatcgcac cctgaggttt atcgtgaccc
       61 caggtatgcg gatcaatccc ccgaaatgag taatcagcga tccccattcg gggggagtgc
      121 ctcacgatgg aagcactccc cgcctacgaa gctcagctcg taggttttgt tcccagcgac
      181 cccggcgttg cggtgaaata ggcaggacag caaccgtccc acccgctgag ttgtatacct
      241 ttgcgtgacc accacgccct gctttggcca cagtgaagcc attggcttcg atctctctga
      301 ctagcttacg gacttgcttg tgcaactcgt tgcactcctt gtgttcctgt agtagccaga
      361 tcagaccgtt acggtggtct tacgccccct agtgctctac ggattccaga cggttggcgc
      421 gagccatccc gtcagctccc cccgcagttc ctgatgctgg agcacatcgt ccacgacacg
      481 ctcgcgtgcc agtgccagcc cctcaggagt caaccgcgct accttgcgcc agttgttgtc
      541 gtgcatgtgg aacttcgacg catctagatc cttcagcgcc gagaggttct cctggctctg
      601 actcagacca gccacgtcga tcaggtcggt cttcgtgtct agaaccagct tccgacccag
      661 cggaggcaag ccccattcga ggcaggcacc gttgaggatc ggtagatcga acttccggat
      721 gtagtggcct gtcaccatgt cagcctcatc atagagggtg cggaaggcgg acacgattgc
      781 ccgcgcatcc ttctccgtcc accgagagcg tagctcccag gtctgtacag gttccccatc
      841 ccacgcggcg gcgaggatga caacctcaca tccatccgga ttgaagcggc ctccgttgtg
      901 gaagccaatg cgacgagtct cgatgtcgaa gtccaggact cgcatggtct tcgactcgat
      961 cagctcatgc actgattgcc ctcagcaggc cagcctcatc tagagcttcg gcaagactca
     1021 ggatcttgcc ctgggctaga gacacctcga accgctcctg cagcgtgggc aggatttcgt
     1081 tcttggcttc ggccttctgc tgagccgcct ctagattgag gcggtattct acctctctcc
     1141 ggatgcgatc ccaggcgact tcagcctctg tcttctgttt gtaacgggct atgtttcagc
     1201 tcctccttga tttccgctat gcgaagttct cgttcacgaa ctctgttctg aaggtgccag
     1261 atacgccact ttggactatg cctatatctg tcctttgcgg cctgcccctt agtggagttc
     1321 cagtagcgac gctgagagtc acgtcctttg tccgtctctc tataacgctg ttgcatagcc
     1381 ctataacgca gcttgcagcg ccactgtcct ccttggggct tcttcttatt caccgtccac
     1441 aacataggtt cgccatgaca cttacatagc ttggtcatca ttattcatga cggatttcag
     1501 cacttctatc gcgcggtcgt aacggcggga gacggtcatg tgactgacgc ctagaaactc
     1561 cgctgcctct ctcgttgtgt agccgcagat accacacatc aaccaggtga cgtactcgtc
     1621 atcggtcagg tactgctgtg cgatatctag atcgactagc tcaacgagca gggacagcgg
     1681 gcgtccaggc ttcgtgtcct tcatgtgccg caaggaacgg tatccctcgc tgagatagcg
     1741 aactctcttg cgggagtaac cgaccttatt taggagcata gaagctctct gaccaacctc
     1801 cctgaggcac gtcattcgag tcagtccagt gttccttccg tcgtccggga tcagaatgaa
     1861 tcccgctttc atcgagcatg tcctcactca tcgactcctg ccgtctccat cgacggcatt
     1921 tggcgatcca gttgaacata tctcgctcga tctctagatt ggatggtctg cgttcttccc
     1981 gcactgcgag cagaactgtg atggaacctt cctggtagag atcatcgaac tctgcgccct
     2041 ccaagccgct atacctgcga gctaggtggg cgacgcgcgg tagatgttcc gccacagcct
     2101 ccgcttcgga ggcaatggca tccggcatta gaagggagtt cccgaacctc cggacacgcc
     2161 gctttgcacg acctgtcggg tgtcctcctt ctccacgcag ggcaagtgga ctagatggat
     2221 cgggttgagc tgcaggttct cacgctggct accgtcctgc gcctggtagc tatacgtact
     2281 gaacgaaccg tcagcggcga tgaagtcgcc cttcttcggc tcagccgtga gcttgaactc
     2341 cggccagatc gtgatggaga ccagcttctg cgaacccgca gtgcggatca ctacgtccgt
     2401 gacctgcttg ccgttcacct cacgcggctt cggaccgaac tggatgatgc cggatgcacc
     2461 ggcatactgc ttgtcagcta tgacttacct ccttatagat cctgcgaccg atcaatacgg
     2521 tctaggctca gtatgtgtgt ctcaacgcgg cgaccttcgg gcaaccggat cacggcccaa
     2581 tctccatcta gagatacgac cgttcctcgg gtgcccctag ctagatcgcc gtgatctaga
     2641 gggatgcggt gtcgcgtctg cacgtaatca cctgctctca tttacctccc tacttgcggc
     2701 ttctagctcc cagcgcccga gcattgctag atcgtgcagc ctcaggaagt cggcatagtc
     2761 catcctcaca gccgctctac caccacgctt cttctctttg atgaagactg cggggatctt
     2821 gttgccgggg atgttcttcg cgttgttcct ggcctgctcc atgtcagctt ccaagaagat
     2881 gaacttctcg tatagcttgc actcaggagc gattagtgga gcgtcgatgc tgtcggggag
     2941 atcatgaccg cgtggccctg tgcgtgtccc gcccagatcc tttgcgacct ttctctccca
     3001 tgccttccat ggcttatccg ccattgatga tgtcctcggg tgtgatgtca tcgaaggtgc
     3061 catccaccac gaactcctcg acccaccaca tcttccgact gtaggagtaa ccctgcagct
     3121 cgtagaagcg gttgttgacg aagacgatgg agccatcctc taggagcgag tagcccgagt
     3181 cgatcaggac agcgtgcgga acccacatct tcccggcgtc tatatggaag gcgatctcca
     3241 gatcggaggg atgttgaggc gcataggcaa ttgcctgctg ctcctcttcc tcgtcaccta
     3301 gagcacgcga taccttgtca cgccagtctg tgtctgggcc tgacttccgc atcccccatg
     3361 tgatctgccc acagatcggg cacttctcca tgttcggggg atactggact gagcacatgg
     3421 tgcatcgacg tgacaggaag ctcccgtcgg tgccgaagat tggcactaga acggaggctc
     3481 cggattcacg ccacctatca gactgacccc tccttcgtgg tcatcgacaa tggctacatg
     3541 cagcacgtct cctgcctggc ggaagcgtga cttgaacgtg gctagtgtca tcgagttgat
     3601 tccggactgg cgagcatcga tcccaacatc gacgttggca acgatctcgc ctgagccgcg
     3661 tgagcggcgg taactgctag tcgattcggt cttgcctgcg tggtgcagca cgacgagtgc
     3721 cgcgcctgtc tcacgagcca gcggcttcag cgccatgttg tagagccgag ccatcggccc
     3781 agccttgtcc tcgtcctgca tgtggaaccg cgtcagtgag tcgagcacga tcagtgcagg
     3841 ctcgaactca agcgcctcgt ctacgagggc atcgggatcg cggtcgagga ggattccctg
     3901 attgctcagg tagcgcagct gcctagcttc ctcgtccgtc atgccgagct tccggcgtaa
     3961 acggtcgaag atcagtacct ctgcgttctc ctcatctgca tacaggacac gaccaggggc
     4021 atctaccttc tggcccagcc agtccgtccc acgaatgacg cctagacaca gcgccatcgt
     4081 gagccagctc ttgccaatcc caggctctcc cgtgatcgtg tgagtagctc cacgcacgat
     4141 catgttctcc actagccagg gcacaggcgg cggagcctgc gtcaggtcta gaggatgcca
     4201 gcgggactgt cccgtaccac tctcggcggc gcggaacaag tggtcgagga tgtagcccgc
     4261 gtcgaagaac tcgcagatgt cttttacgtc accagggagt cgcacacgac gcgccttgcc
     4321 tccgagcgag gcccggatgt tgcgccaagc cgtgtccacc ctggcttgca cgttgtagtc
     4381 ccggtcgttg tcgaggatga cgtagaccct ctgccccgta aaggcggcag ctagctcatc
     4441 gcgccatgtc tcgatgccgg acagacctac aacacctcgt gtggttccag ccgagctgtt
     4501 gttgtcccac agctcctgcc acagccgcat cgtgtctgtc tcgccctcga ccaggaacac
     4561 cgtcgtcttg ctgg
//
LOCUS       DATMTI010000544         1987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_698862, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000544 DATMTI010000000
VERSION     DATMTI010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698862"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..141
                     /locus_tag="VN039_08710"
     tRNA            66..141
                     /locus_tag="VN039_08710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Ala,seq:ggc)
     gene            446..>1987
                     /locus_tag="VN039_08715"
     CDS             446..>1987
                     /locus_tag="VN039_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWV46084.1"
                     /translation="MLQIESISKQYSTKVLLTEASAHLRPNSRVGLVGPNGAGKTTLF
                     RMVLGEESPDKGSIRKRPRLRIGYLPQELETITGKTVLDAAHRDEYPEHEAKRILMGL
                     GFTEVDFDRHVEKLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFEQFLLQ
                     SGMTLLIISHDTAFLDRVVTHIWELRHHKIEEYRGNYSLFRKLKAERDAQLHASAARQ
                     SKEIARVQKFVDRFRYQANKASQVQSRLKQLDKVKRIEIQRDPKRVKFRFPLPSASGR
                     QVLELSGASKRYGEKVVYKTLDFSVERGQRIALVGENGAGKSTLLKMLAGVLPLDTGT
                     RTVGHGVSLHYFAQHQAETLNPEHSILESLEEVTKHAEMNFLRGIAGAFLFSGQDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDVLTDALAEFPGTIIFIS
                     HDPTFLARIATRVVEIEEGLARNYIGDYEYYLWKKAQEFESIKETSEELEGAAQSTSS
                     GPTRAM"
BASE COUNT          447 a          556 c          538 g          446 t
ORIGIN      
        1 cattcgttgt ttgtgacggc cttgagttct tgtagttcac tcatcgttca tcattgactc
       61 gaagtggggc tgtggcgcag ttgggagcgc gcgtgaatgg cattcacgag gtcgccggtt
      121 caatcccggc cagctccacc aaaccaagct tcgcttgacc cggtcgctaa gtttcgtagt
      181 attcgtaatt ggcgcgatcg gaaagagtct tcaacccacg ttggcgttta aacgttccgc
      241 tgcgtgtgcc ggccgctctt ttgagatgta tcgtaatcgt cacggccgga tagccgactc
      301 acgcatctaa agattcggat tctagaatgc ttactgtgtt taatttcatc acattgcctc
      361 gcctcccaac ctcctagcca ttcacgttgt tcgcgtccat ctcgggtgac cgagtcatcc
      421 catttcacat ggaaggtttg tgccgatgct ccaaattgaa tccatcagca agcaatattc
      481 cacgaaggtg ctgctcactg aggcctccgc gcatcttcgc ccgaattcac gagtcggttt
      541 agtcggaccg aacggcgccg ggaaaacgac gctctttcgg atggtccttg gtgaagagtc
      601 accggacaaa ggctcgatcc gcaagcggcc tcggcttcgg atcggctacc tcccgcagga
      661 gctcgaaaca attactggga agaccgtcct cgatgccgcg catcgcgatg agtaccctga
      721 gcacgaggcc aagcgtatct tgatgggact ggggtttacc gaggtcgatt ttgatcgaca
      781 tgtcgagaaa ctgtccggag gctaccggat gcgggtcgcg ctggcacatc tgctcttatc
      841 gaaccccgat gtgttgatgc tcgacgagcc gaccaaccat ttggataaac cgacccagcg
      901 ttggttcgaa cagtttctgc tccaatccgg catgacgctc ctgatcatca gccacgatac
      961 cgcgtttctg gaccgcgtgg tcacccacat ctgggaattg cgtcatcaca agatcgaaga
     1021 ataccgaggc aactactcgc tcttccgaaa attgaaagcg gagagagatg cccaactaca
     1081 cgcctcggcg gcgcggcagt ccaaagagat tgcccgtgtt cagaagttcg tcgatcgctt
     1141 ccggtaccaa gccaacaagg ccagtcaggt tcaatcgcga ctgaagcagt tggacaaggt
     1201 caagcggatt gaaatccaac gagatccaaa acgagtcaag ttccggtttc cccttccttc
     1261 tgccagcggc cgtcaggtgc tcgagttgtc cggagcgagc aaacgctacg gtgaaaaggt
     1321 cgtctacaag acactcgatt tttccgtgga gcgcggccaa cgtatcgcgc tggtcggtga
     1381 aaatggggcg ggaaaaagca cgcttctgaa gatgctcgcc ggtgtgcttc ctcttgatac
     1441 cggcaccaga accgtggggc atggggtgag cttacactac ttcgctcagc atcaggccga
     1501 aaccttgaac cctgagcact cgattcttga atctcttgaa gaagtcacca aacatgcaga
     1561 gatgaatttt ctgcgcggga tcgcaggcgc gttcttgttt tccggccagg atcagaaaaa
     1621 accgatcaag gcgttgagcg gtggtgagcg gaaccgggtg gccttagccc gcatgctggt
     1681 cgaaccggcc aatactttgc ttctcgacga gccgacgaac catttggatc cggcctccgt
     1741 agatgtgctc accgatgcat tggctgaatt ccccgggacc atcattttca tctcccatga
     1801 cccgacgttt ctggcgcgca tcgccacccg ggtcgttgag atcgaagagg gactggcgcg
     1861 aaactacatc ggcgattatg agtactatct gtggaagaag gctcaggagt ttgaatcgat
     1921 caaagaaacg agcgaagaac tcgaaggagc cgcgcaatcc acttcctcag gccctacccg
     1981 cgcaatg
//
LOCUS       DATMTI010000545         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_20681, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000545 DATMTI010000000
VERSION     DATMTI010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20681"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1580
                     /locus_tag="VN039_08720"
     rRNA            <1..>1580
                     /locus_tag="VN039_08720"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          424 a          356 c          523 g          277 t
ORIGIN      
        1 acggcggaac acgtgaaatt ccgtcggaag ctgggaggac catctcccaa ggctaaatac
       61 tacctagtga ccgatagtga accagtaccg tgagggaaag gtgaaaagca ccccggaagg
      121 ggagtgaaag agaacctgaa accgtgtgcc tacaagtagt cagagcgcgt tcatgcgtga
      181 tggcgtgcct tttgtagaat gaaccggcga gtgacgatga cgtgcgaggt taagccgaag
      241 aggcggagcc gcagcgaaag cgagtctgaa cagggcgaaa agtacgtcgt cgtcgacccg
      301 aaaccaggtg atctacccat gtccagggtg aaggccgggt aacaccggct ggaggcccga
      361 acccacgcac gttgaaaagt gcggggatga ggtgtgggta ggggtgaaat gccaatcgaa
      421 cttggagata gctggttctc cccgaaatag ctttagggct agcctcggga tggagagtgt
      481 tggaggtaga gcactgattg ggtgcggggc ccgccaaggg ttaccaagtc cagtcaaact
      541 ccgaatgcca tcgacttata gccgggagtc agacagtgag tgctaagatc cattgtcaag
      601 agggaaacag cccagatcat cagctaaggt ccccaagtgt gtgttaagtg ggaaaggatg
      661 tggagttgca aagacaacca ggatgttggc ttagaagcag ccaccattta aagagtgcgt
      721 aatagctcac tggtcgagtg actctgcgcc gaaaatgtac cggggctaaa cgtgccgccg
      781 aagctgcggg atgaccgttg gtcatcggta ggggagcgtt ctaagggcgt tgaagccaga
      841 ccggaaggac tggtggagcg cttagaagtg agaatgccgg tatgagtagc gaaaacagag
      901 gtgagaatcc tctgcgccga aagcctaagg gttcctgagg aaggttcgtc cgctcagggt
      961 tagtcgggac ctaagccgag gccgaaaggc gtaggtgatg gacaacaggt tgagattcct
     1021 gtaccacctc cttcccgttt gagcgatggg gggacgcagg aggatagggc gagcaggcgg
     1081 ctggaagagc ctgtccaagc cgcaaggctg atccgtaggc aaatccgcgg attgtaaggc
     1141 caagcggtga tggcgacgga gtcatccgga agtccccgat ttcacactgc caagaaaagc
     1201 ctctagcgag ggaagaggtg cccgtaccgc aaaccgacac aggtaggcga ggagagaatc
     1261 ctaaggcgcg cgggagaact ctcgttaagg aactcggcaa aatgaccccg taacttcggg
     1321 agaaggggtg ctcgtttggg tgaagagccc gaacgagccg cagtgaaaag gcccaagcga
     1381 ctgtttatca aaaacacagg tttctgcaaa atcgtaagat gaagtatagg ggctgacgcc
     1441 tgcccggtgc tggaaggtta agaggagtgc ttagcgcaag cgaaggtgcg aattgaagcc
     1501 ccagtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
     1561 gttccgaccc gcacgaaagg
//
LOCUS       DATMTI010000546         1919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_190889, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000546 DATMTI010000000
VERSION     DATMTI010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190889"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /gene="dnaA"
                     /locus_tag="VN039_08725"
     CDS             <1..1105
                     /gene="dnaA"
                     /locus_tag="VN039_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWV46085.1"
                     /translation="REKRATLPGEPQAVVNGPRAVTQSKPRRGIQLNPKYIFKNFVVG
                     AGNQFAHAACMAVAEQPGHTYNPLFIYGGVGLGKTHLLNAIGNHVAEKSDLRIAYLTT
                     EQFTNEVINSIRYDKMMDLRKRYRHIDMLMIDDIQFLVGKERTQEEFFHTFNALYEGH
                     KQIVLSSDRFPKDMPDIEERLRSRFEWGLIADLQPPDVETRIAILRKKSEDEGVKLPE
                     DVIQFLSITMKSNIRELEGSLVRLGAYASLTGQVITLDLAKNVLRDLIGDKKKIVAMD
                     DIQEAVCAQFHVKMMELKSRRRSKTLVHPRQIAMYLCRELTDASYPEIGRQFGGKDHT
                     TIIHACRQVAKAKETDTMLQTTIETLKEQILRN"
     gene            1129..>1919
                     /gene="dnaN"
                     /locus_tag="VN039_08730"
     CDS             1129..>1919
                     /gene="dnaN"
                     /locus_tag="VN039_08730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWV46086.1"
                     /translation="MKVRIGRDELLTGLQRVQGVVEKRNTMPILSNILLEAKQDGVEI
                     VATDLEIGMRGLYKGTVLSPGGVTISARKLFEIVKELPPGEIELTATDNSWTTIQAGK
                     SQFKVVGLPSSEYPALPTIDREGLTPLSGEGLLELIRKTLFAAGDNDARYILNGLLVT
                     LAATDKKTSLRLVGTDGHRLAVAEQEVGKAGSKGLPQEIKAIIPKKAAHEIRHLLEEG
                     GDAEPLIGFSKNLMIFRKSGLLLTSRLMEGNYPNYQQVIPKENGK"
BASE COUNT          513 a          484 c          531 g          391 t
ORIGIN      
        1 ccgtgagaag agagctaccc tacccgggga gccccaagcc gttgtcaatg gacctcgtgc
       61 cgtaacgcag tccaagcctc gccggggaat ccagctgaac ccaaaatata tattcaagaa
      121 ttttgtcgtt ggtgctggga atcagttcgc tcatgcggcc tgcatggcgg tggccgagca
      181 gcccgggcat acgtataacc cgctctttat ttacggtgga gtgggactgg gaaagaccca
      241 cctcttaaat gccatcggaa atcatgtggc tgaaaagagc gatctgcgca tcgcatatct
      301 gaccacggag caatttacca acgaggtgat caactctatt cgctacgaca agatgatgga
      361 tttgcggaag cgctatcgac acatcgatat gctcatgatc gatgatattc agttcctagt
      421 cgggaaagaa cggacacagg aagagttctt ccatacattc aatgcgttgt atgaagggca
      481 taaacaaatt gtcctctcaa gcgatcgttt tcccaaagat atgccggata tcgaagagcg
      541 cctacgatct cgctttgaat ggggactgat tgcggacttg cagccgcccg atgtggaaac
      601 ccgcatcgcc atcctgagaa aaaagtcaga ggatgaggga gtcaaactgc ccgaagacgt
      661 catccagttc ctgtcgatca cgatgaagag caatatccgc gagttagaag gtagtctggt
      721 tcgattgggt gcgtatgcct cgttgaccgg gcaagtcata acccttgatc ttgccaagaa
      781 tgttctccgt gatctcatcg gggacaagaa aaagatcgtt gcgatggatg atattcaaga
      841 ggcggtctgt gcacagttcc atgtgaagat gatggagctg aaatcccgcc gccggagcaa
      901 aacactcgtg catcctcgac agatcgccat gtatctgtgt cgcgagctga ccgacgcatc
      961 gtaccctgaa atcgggcgcc aattcggggg gaaggaccat acgaccatca ttcatgcctg
     1021 tcgccaggtc gcaaaggcca aggagacgga cacgatgttg cagacgacga ttgaaacatt
     1081 aaaagagcaa attcttagaa actaagagct tggccgggag agatctcaat gaaagtacgt
     1141 attgggcgag atgaattgtt gacaggactc cagcgggtgc aaggtgtcgt ggagaaacga
     1201 aacacgatgc cgattctgtc gaacattctg ttggaagcca agcaggatgg ggtggagatc
     1261 gtagcgacgg accttgagat cggcatgcga gggctgtaca aagggaccgt tctttcgccc
     1321 ggcggtgtca ccatctcggc ccgaaaacta ttcgaaatcg tcaaagagtt acctcctggc
     1381 gagatcgaac tgacggcgac tgacaatagc tggaccacca tccaggccgg gaagagtcaa
     1441 ttcaaggtcg taggcctccc cagcagcgag taccccgcac tgccgacgat cgatcgtgag
     1501 ggattgacac cgctctcggg agaaggactt ctggaattga tccggaagac actctttgcc
     1561 gccggagata acgatgcccg ctatatcctg aacggcctcc tagtcaccct cgctgcgacc
     1621 gacaaaaaga cctcccttcg attagtcggc acagatggcc atcgtttggc ggtggcggaa
     1681 caggaggtcg gcaaggccgg cagtaaaggc ctgccgcaag aaataaaagc catcatacct
     1741 aaaaaggccg cgcacgaaat ccgccatctg ttggaggagg gcggagacgc cgaaccgctc
     1801 atcggttttt caaagaatct catgatcttc cgcaagagcg gcttgctgct gacgtcgcgg
     1861 ttgatggaag gcaactatcc taactaccaa caggtcattc caaaggaaaa cggcaagaa
//
LOCUS       DATMTI010000547         6193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_444660, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000547 DATMTI010000000
VERSION     DATMTI010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444660"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..547)
                     /gene="cysC"
                     /locus_tag="VN039_08735"
     CDS             complement(17..547)
                     /gene="cysC"
                     /locus_tag="VN039_08735"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWV46087.1"
                     /translation="MRDHSSFAIWLTGLPASGKSTIVAALKPQLEAMGLSVEVLESDA
                     VRRTLTPSPTYSQLERDLFYRALAFTGAKLVLHGVTVIFDATANRRAYRDFARCLITK
                     FIEVWVECPLELAMQRDYKGTYQRGQRGESSTVPGLQEPYEAPLNPEVRIDTTKMNAN
                     EAAEMIVKLVNERCLR"
     gene            complement(544..1632)
                     /locus_tag="VN039_08740"
     CDS             complement(544..1632)
                     /locus_tag="VN039_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWV46088.1"
                     /translation="MPKLSKQALEKYLRTRFGSHVKLRSYGIIGKESSKGEQKRYGYG
                     TPVKLTFQIGRRVQSAVLQTMKPGPFGHEHMADRAQAMLWDYDSYGRLSRHVKALDVG
                     AFDTNQTLFSLAEARELFVLNEWTDGVSYHGDLERLMKDGMLRKLDRQRTVALARYLA
                     QIHAKKRRDPDLYKRRLRELIGHGECIMGLTDSYPARCGFITGDLLRTVEEACNRWRW
                     RLHDKTNRLSQVHGDFHPYNVLFRTGTDFAVLDRSRGEWGEPADDITAMTINYLLNSL
                     IRWGKLKGPFEVLFRLFWDTYVEISGDKEVTETAAPFFAFRGLVVASPLWYPNLSIAI
                     RRSLFHFIENVLDVSCFEPERVNEYCQR"
     gene            1915..2565
                     /locus_tag="VN039_08745"
     CDS             1915..2565
                     /locus_tag="VN039_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV46089.1"
                     /translation="MPKKASISRVKRAAESPARNVFPQRIVSESRRHFFANGFRNVTM
                     DDLAHELAMSKKTLYACFPSKTSLVEAAIVDKFKNVDFDLSRITSTSSSDCLAALRLL
                     LECVQGHMEEIRPPFLRDIRRETPELFAVVEGRRRDLIQRHFGKVFQEGRRAGLIRHD
                     IPSKLAIEILLGAVQAVMNPPKLEELGLTPKSGFTSIMRVVLEGLTTEKARSHRPG"
     gene            2579..3577
                     /locus_tag="VN039_08750"
     CDS             2579..3577
                     /locus_tag="VN039_08750"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWV46090.1"
                     /translation="MKRRVLIIGLAVLAAIAGGYLYVGSFAERATDNTLRIAGNIEAH
                     ESVVGFKVPGRIVELPVQEGQYVNKGDLLARLDDEDYRQQVSVDEATIRTREAELKLA
                     LAGSRKQEIQAAKQSLIDAQSDRELKRAEFRRRQALLSEQAVSQEDVHSAETLMKRAE
                     ATYQRIKETYDQIVEGTRKEEIAVRRANLQLAQEHLEMAQVNLSYTVLTAPISGVVLV
                     RQAELGEVVAPGTPVVTIADVDRLWMRGYINETDLGRVRWDQPATVRTDTYPGKDYHG
                     RVSFIASQAEFTPKSVETYKERVTLVYRIKVDLENQNHELKPGMPAEATIDVPSKQ"
     gene            3581..4510
                     /locus_tag="VN039_08755"
     CDS             3581..4510
                     /locus_tag="VN039_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV46091.1"
                     /translation="MPPSVATSNLTKHFPGVTAVNRLTFEVHAGEIFGLVGPDGAGKT
                     TTLRMLAGVLSPDGGSATVAGADIAREPEAAKARLSYMPQRFGLYEDLTVAENIRFYA
                     DVFGVSRAERETRSRQLLDAAGMSPFTTRLAGKLSGGMKQKLGLVCALIHRPLVILLD
                     EPTNGVDPVSRRDFWRILYSLLNEGIAILMSTSYLDEAERCHRVALLHQGQMLYCETP
                     PGLKRRLDGAVVSIVGHEARRIRTALGGAAGVLNALLVGDGVHLVVDDAGRRIPELRA
                     ALRSAGLSFDRLEEVEPTIEDLFVAATSEERKP"
     gene            4507..5457
                     /locus_tag="VN039_08760"
     CDS             4507..5457
                     /locus_tag="VN039_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV46092.1"
                     /translation="MTDHDTIPVLAHDLVKRFGDFVAVDGINLEARTGEVVGFLGPNG
                     SGKSTTIRMLCGLLRPSAGQALVAGFDVAREPEQVRRRIGYMSQKFSLYNDLQVIENI
                     RFFAGMYDVPASVLHERVQWVLDMAGLAGRESTITGTLPSGWKQRLALGCAVLHRPSI
                     LFLDEPTSGVDPISRRRFWELIHAMAAEGVTVLVTTHYMDEAEYCNRLVLIFQGRIVA
                     AGSPTELKQQAMTGELLLIECDQLGLALDVLRQVPDARDVALFGNALHVVVPDGRTGI
                     PRLHDALSEHGVAVSRIEPIRASLEDVFVSLTARLSGQRV"
     gene            5457..>6193
                     /locus_tag="VN039_08765"
     CDS             5457..>6193
                     /locus_tag="VN039_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV46093.1"
                     /translation="MRLHRLIAIIRKETLQIRRDFASLLITVAMPLLLMLAFGYGVRF
                     DVKHIAVYVYDRAGNQQSQDFLKHFQASEYFRLVKVVDNYPALIEALDSGACRMAIVI
                     PVDFSERLGEGGPVAVQALVDATDNNTANISLNYGEAVAQTYNQRLHLDWVQRHGLNA
                     VQSPLSVDTRTWFNENLESTANIVPGVIAIIMAVIGSFLTSLTIAREWERGTMEQLVS
                     TPITPLELMVGKLVPYFVIGLLDTVLCT"
BASE COUNT         1340 a         1807 c         1742 g         1304 t
ORIGIN      
        1 tccaggagca gattgctcag cgcaaacatc gctcgttgac cagcttcaca atcatctccg
       61 ccgcttcatt ggcgttcatc tttgtcgtat caatcctcac ttcaggattc agcggcgctt
      121 cgtagggttc ctgcaagccg ggtaccgtag aagattctcc tcgttgtcca cgctggtacg
      181 tccctttgta gtcgcgctgc atggccagtt ctaacggaca ttctacccac acttcgatga
      241 acttcgtgat cagacatcgc gcaaaatcgc gataggcccg cctgttggcc gtcgcatcga
      301 aaatgaccgt gacaccatgc aacactaact tcgcgcccgt gaacgccaat gctcggtaaa
      361 aaagatcccg ctccagctgt gagtaggtcg gagacggcgt aagcgtacgc ctcaccgcat
      421 ccgattccaa cacttcaaca gataacccca tcgcctcaag ctgcggcttg agtgcggcaa
      481 cgatcgtgct tttccccgag gcgggcaaac ctgtgagcca aatggcaaaa gagctgtgat
      541 ccctcatcgc tgacaatact cattcactcg ttccggctcg aagcatgaca catcgaggac
      601 attctcgatg aaatgaaaca agcttcgtcg gatggcgatc gacaaattgg gataccagag
      661 gggactggcc acgaccagac cacgaaacgc gaagaacggt gcggccgttt cagtcacttc
      721 tttgtcgccg ctgatttcca catatgtatc ccagaacaac cggaacaaga cctcgaacgg
      781 acccttgagc ttaccccatc gaatcaatga gttgagcaaa tagttaatgg tcattgccgt
      841 gatatcgtcg gccggctcgc cccattctcc tcgcgaccga tccaacaccg caaaatccgt
      901 cccggtccgg aacagcacat tgtacggatg gaaatcccca tgaacctggg aaagacgatt
      961 ggtcttatca tgaaggcgcc accgccaccg attgcatgct tcctccactg ttcgcaacaa
     1021 atcaccggta ataaacccac agcgcgccgg atagctgtcg gtcagtccca tgatgcattc
     1081 gccatgaccg atcaattcac gtagacgacg cttgtataaa tccgggtcgc gccgcttttt
     1141 ggcatggatc tgggccagat aacgagccaa ggcgacggtg cgttgtcgat ccaacttacg
     1201 cagcataccg tctttcatca atctctcaag atctccgtga tagctcaccc cgtcggtcca
     1261 ttcattcagc acgaagagtt cgcgggcttc cgcgagggaa aagagggtct ggtttgtgtc
     1321 gaatgcacct acgtcgaggg ctttcacatg gcgggaaagg cgtccatagg aatcgtagtc
     1381 ccacaacatg gcttgagcac gatccgccat atgttcatgc ccgaatgggc cgggcttcat
     1441 cgtctgaagc acggcggact gaactcggcg cccgatctga aacgtcaatt tgaccggcgt
     1501 gccataccca taacgcttct gctctccttt ggaactctct ttgccgatga ttccatagga
     1561 tcgaagcttg acgtgagagc caaaccgcgt ccgtagatac ttttccagcg cttgtttgct
     1621 caacttcggc ataacgcgac attacgcaag tcatattcca gcttcaccag gcgtgagatg
     1681 actcattaat acaagatcga tcgcgcggac tgtcgcattg agcgccactg atcggcatat
     1741 cattgtggac aaatgcgcca tgcgcacacg ctgattacca gcggttcgaa agaacacggg
     1801 cggctcttac ctcatcccct cagctcgttc tcttgttaca aggaggagaa agcctgaacc
     1861 cttacctgtt tgcatttgac tggaaactga agatactagt atagtttcct cagtatgcca
     1921 aagaaagcgt ctatatcgcg cgtgaagcgg gcggctgaat cgcccgcaag aaacgtcttc
     1981 cctcaacgca tcgtcagtga atctcgacgg cacttcttcg ccaatgggtt ccggaacgtc
     2041 acaatggacg atctggccca tgagttggcc atgagcaaaa aaacgctcta cgcctgtttt
     2101 ccgagcaaga cgtcgcttgt ggaagcagcg atcgtggata agttcaagaa cgtcgatttc
     2161 gatttgagcc gcattacgtc tacgtcctcg tccgattgcc tcgcagcgct gcgtctcctc
     2221 ctcgaatgcg tgcagggaca catggaggaa atccggccgc ccttcttacg tgacatccgc
     2281 cgtgaaacgc cggagttatt cgcggtggtg gaaggccgga ggcgagactt gattcagcgg
     2341 cacttcggca aagtattcca ggaaggtcgc cgggcgggac tcatccgcca tgacattcca
     2401 tccaagctgg cgatcgaaat cctcctgggc gcggtgcagg ccgtgatgaa cccgcccaag
     2461 ttggaggaac tgggtctcac gcccaaaagc ggctttacct ccattatgcg ggtagtactg
     2521 gagggattga cgacggagaa agctcggtca catcgaccag gttgacattg gtacagaaat
     2581 gaagagacgg gtcttgatca tcggtcttgc agtcctagcg gcgatcgccg gtggatatct
     2641 ctacgtgggc tcattcgccg agcgggcgac cgacaatacg cttaggattg cgggcaacat
     2701 cgaagcgcat gagagtgtgg tgggctttaa agtaccgggc cgcatcgtgg agctgccggt
     2761 ccaagagggc caatatgtca acaagggcga tctgttggcg cgactggacg acgaagatta
     2821 ccgccagcaa gtgagcgtgg acgaggcgac gatacggaca cgagaggctg agctgaagtt
     2881 ggccttggcc gggagccgta aacaggagat ccaggcggct aaacaatctc tgatcgacgc
     2941 gcagtcggat cgcgaactca agcgtgccga attccgccga cgccaagccc ttctcagcga
     3001 gcaagccgtg tcccaagagg acgttcacag cgcggaaaca ttgatgaaac gagcagaggc
     3061 cacctatcaa cgcatcaagg aaacttatga ccaaatcgtc gagggcaccc gcaaggagga
     3121 aatcgccgtg cggcgtgcca atcttcagct ggcccaagag catctggaga tggcgcaagt
     3181 gaatctgtcc tacaccgtcc tcacggctcc gatctccgga gtggtcttgg tgcggcaggc
     3241 cgagctgggc gaggtcgtgg caccaggaac acctgtcgtc acgatcgccg atgtcgaccg
     3301 gctctggatg cgaggctaca tcaatgagac ggatctgggt cgagtgcggt gggatcaacc
     3361 ggctaccgtg cgcactgaca cgtacccagg caaggattac cacggtcgcg tttcgttcat
     3421 cgcgtcccag gccgagttca cgccgaagag cgtggaaact tataaggagc gtgtgacgct
     3481 cgtctatcgc atcaaggttg atctagaaaa ccaaaaccat gagctcaagc cggggatgcc
     3541 ggctgaagcg accatcgacg tgccgtcgaa gcaatgacct atgccgcctt ccgttgccac
     3601 atcgaacctg acgaagcact ttcccggagt cacggccgta aaccgcctga cgttcgaggt
     3661 acatgcaggt gagattttcg ggttggtggg tcctgatgga gcaggaaaga cgacgacgct
     3721 tcgcatgctg gccggcgttc tttcaccgga tgggggatcg gccaccgtcg cgggcgccga
     3781 tatcgcccgc gagcctgaag cggccaaagc ccgcctgagc tatatgccgc aacgtttcgg
     3841 actctacgaa gatctgaccg tcgcggagaa cattcgtttc tatgccgatg tgttcggcgt
     3901 gtcgcgcgcg gaacgagaga cgcggtctcg gcaattgctc gatgccgccg gcatgagtcc
     3961 ctttacgact cgattggccg gcaagctttc cggaggaatg aaacaaaaac ttggtctcgt
     4021 ctgtgccctg atccatcgcc ccctcgtaat cctgctcgat gaaccgacca acggcgtcga
     4081 tccggtctca cgacgagatt tctggcgcat cctgtactca ctgctgaacg aaggcattgc
     4141 cattttgatg tcgacttcct atctcgacga agccgaacgc tgccatcggg tggcgctgct
     4201 ccatcaggga cagatgctgt attgcgagac gcctcctgga ttgaagagac gcctcgacgg
     4261 cgcggtggtg tcgatcgtcg ggcatgaggc ccgtcgtatt cgaaccgccc tcggcggagc
     4321 agccggcgtg ctcaacgcct tgctcgtcgg cgatggggtc catctcgtgg tggacgatgc
     4381 tgggcgtcgc attcccgagc ttcgtgcggc actccggtcg gctggtctgt catttgaccg
     4441 cttggaagaa gttgagccga cgatcgaaga cctgttcgtc gccgccacct ccgaagagcg
     4501 gaaaccatga cggatcacga tacgataccg gtgctcgcgc acgatcttgt caaacgcttc
     4561 ggcgacttcg tcgcggtaga cggcatcaat ctggaagcgc gaaccggaga ggtcgtaggc
     4621 ttcttgggcc cgaacggctc cggcaaatct acgacgatcc gcatgctctg cggcctgctt
     4681 cgcccctccg ccggacaggc cttggtggcc ggctttgatg tcgccaggga gcccgaacag
     4741 gtccgccgcc ggatcggata tatgtcgcag aagttttcgc tctacaacga ccttcaagtg
     4801 atcgaaaaca tccggttctt tgccggcatg tacgacgtgc ctgcgtcggt ccttcatgaa
     4861 agggtgcagt gggtgctcga catggccggc ttggccggtc gcgaatcaac gatcaccggc
     4921 acgctcccca gcggctggaa acaacggctc gccttaggct gcgccgtgct ccatcgaccg
     4981 tcgattctct tcctggacga accgacatcc ggcgtcgatc cgatctcgcg tcgtcgattt
     5041 tgggagttga tccatgcaat ggcagctgaa ggagtgaccg tgctcgtcac cacgcactac
     5101 atggacgagg cggagtactg caatcgtctc gtgctgatct ttcaaggccg tatcgtggcc
     5161 gccggttcgc cgaccgagtt gaaacagcaa gccatgaccg gcgagctgct tttgattgaa
     5221 tgtgatcaac tcggcctggc gctcgatgtg ctgcggcagg ttccggacgc ccgagatgtc
     5281 gccttattcg gcaatgctct gcacgtcgtc gtgccggacg gacggaccgg tatcccgcgc
     5341 ctccacgacg ctctgtcgga acatggggtt gcagtgtcga ggatcgaacc tatccgcgca
     5401 agcttggagg atgtcttcgt ctcgttgacc gcgcgattga gcggtcagag ggtgtgatgc
     5461 gcctgcaccg actcatcgcc attatccgca aggaaacgct ccagattcgc cgcgacttcg
     5521 ccagcctgtt gattacggtg gcgatgccgc tgctcctcat gctggccttt ggctacggcg
     5581 ttcgtttcga cgtcaaacac atcgcggtgt atgtgtacga ccgagcgggc aaccagcaaa
     5641 gtcaggactt tctaaaacat tttcaagcgt ccgagtattt tcgcctcgtc aaggttgtgg
     5701 acaattatcc ggcgctgatc gaagcgcttg actcaggggc ctgccggatg gccatcgtca
     5761 tcccggtcga tttctccgag cggttgggcg agggcggccc cgtcgcggta caagcgctgg
     5821 tcgacgccac cgacaacaat accgccaata tcagtctcaa ctacggtgaa gccgtcgctc
     5881 aaacctacaa tcaacgcctc catctggact gggttcagcg gcatgggctg aacgcggtcc
     5941 aatctccgtt gagtgtcgat accaggacct ggttcaacga aaaccttgaa agcaccgcca
     6001 atatcgtccc gggggtcatc gccatcatca tggccgtcat cggttccttt ctcacttcac
     6061 tgacgatcgc gagagagtgg gagcggggca ccatggaaca gctcgtctcc acgccgatca
     6121 cgcccctcga actcatggtg gggaaactcg tgccgtattt tgtgatcggg ctgttggaca
     6181 cggttctctg cac
//
LOCUS       DATMTI010000548         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_190931, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000548 DATMTI010000000
VERSION     DATMTI010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190931"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VN039_08770"
     CDS             complement(<1..318)
                     /locus_tag="VN039_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV46094.1"
                     /translation="MTEPSSHTVLILGSGLAGLATAYHLHQRGYHITLLDHADWLDGF
                     RTDASQPAPIVLGCHHETRRILCALGREWPSEADRTIPLGFRLPNGRIFPYQATRLPG
                     ALQW"
     gene            complement(315..1175)
                     /gene="hpnD"
                     /locus_tag="VN039_08775"
     CDS             complement(315..1175)
                     /gene="hpnD"
                     /locus_tag="VN039_08775"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HWV46095.1"
                     /translation="MTVSEAQAYCTAYTKKSGSNFYYSFLFLPKAKRDAMYTVYAFCK
                     AVDSAVDEPAAGINPKDELKRWRDEVDAVYSATPTTPIMVSLAHHVKVLGIPKAYFEE
                     LIKGVEMDLLNNRYVTFDELSLYCYRVASVVGLICLHVFGVTSARAQDYAVALGMAFQ
                     LTNILRDVGTDAAESRIYLPLDDLRKWNHPEKAILNRSYSPEFRALMEYEASRAHHYY
                     KRADAALMGLSPQERRALTVAEIMRGIYSRILERIERSNYQVFGPRISLTTIQRMVIA
                     LRVWLRSRFS"
     gene            complement(1172..2113)
                     /locus_tag="VN039_08780"
     CDS             complement(1172..2113)
                     /locus_tag="VN039_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HWV46096.1"
                     /translation="MRICSLIPGATEVVAALDLADQLVGISHECDFPASVRHVPVMIE
                     PLVRKDGGSGKEIDSQVKELVASGQRLYRLNEDAFRRASPDLILTQDLCHVCSVTPDQ
                     LTRAIASLQHRPEVLTLSPTMLDDMIHDIERIAEAAGVPAKGLALTQELRRRVTRVRA
                     ETDRTASRPRVVCLEWLDPLYVAGHWVPEMVELAGGRNVLGSQDAPSHETTWQAVEAA
                     RPDVILVMPCGYSVERTVTELRRVGQTGDAWRRACEQRPDLYVVDAASFFSRPGPRLV
                     DGVELLAAILHPTPDHPIDSVKAIKLEATTLTAGRAS"
     gene            complement(2117..2809)
                     /locus_tag="VN039_08785"
     CDS             complement(2117..2809)
                     /locus_tag="VN039_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWV46097.1"
                     /translation="MRAVIFDFDGVIADTEPLHFESLRRTLAGIEIVLTETDYYADYL
                     GFDDRGCIQEALRINRRPISPSLVEELMAKKSAVYLTSIKDHLVIFPGVREFVENAAA
                     TCPIAIASGALRAEIELVLEQIGIRKAFRHITSAEDVTCGKPNPEPFLQALAGLNRRH
                     SAAPIPPDACLAIEDSRPGIRAARSAGMKVLAVANTHTVQDLHEADAISYSLGETRLK
                     DLHARLWPTEAI"
BASE COUNT          607 a          795 c          903 g          615 t
ORIGIN      
        1 ccactgcaag gctccgggga ggcgggtcgc ctgatagggg aagattcgcc cgttgggcag
       61 ccgaaatccc agtggaatcg ttcgatcggc ttccgaaggc cattctcgac cgagcgcaca
      121 aaggatccgc ctcgtctcat gatggcatcc cagaacaatc ggggcaggtt gtgatgcatc
      181 ggtccggaac ccatcgagcc agtcggcatg atcgagtaag gtgatgtggt agccccgttg
      241 atgaagatgg tacgcagtgg ccaggccggc cagtccggaa ccgagaatga ggacggtgtg
      301 tgaggatggc tcggtcacga gaatcgggag cggagccaaa cacgtagggc aataaccatt
      361 cgttggatgg tggtgagact aatacgcggt ccgaagacct gatagttcga ccgttcgatc
      421 cgttccaaaa tccgactgta aatgccacgc atgatttctg caaccgtcag cgcgcggcgt
      481 tcctgaggcg agagtcccat gagggccgca tcggctcgct tataatagtg atgcgctcgt
      541 gaggcctcat attccatcaa tgcacggaac tccggtgagt agcttcgatt gaggatggct
      601 ttttccggat gattccactt ccgtaagtcg tccagcggca gatagattcg actttcggcg
      661 gcatccgttc cgacatcacg gaggatgttg gtcagttgga aggccatgcc gagcgccact
      721 gcataatcct gcgcgcgcgc cgatgtgacg ccgaagacat gcaaacagat gagccccacc
      781 acggaggcga cgcgatagca atagagtgac agctcatcga atgtgacata gcgattgttg
      841 agcaaatcca tttcgacgcc tttgatcaat tcctcgaagt acgctttcgg aatacctaag
      901 acctttacat ggtgggcgag gctcaccatg atgggtgtgg taggggtagc cgaatagacc
      961 gcgtccacct cgtcacgcca acgcttgagt tcgtctttcg ggttgattcc ggcggccggt
     1021 tcatcgacgg cgctgtcgac ggccttgcaa aaggcataga ccgtgtacat ggcgtcgcgt
     1081 ttggctttag gaaggaacag gaacgagtag taaaaattgc tgccgctttt cttcgtgtag
     1141 gccgtgcaat aggcttgagc ttcagagacc gtcatgacgc gcggcctgcg gtgagggtcg
     1201 tggcctcaag tttgatcgct ttgactgagt cgataggatg gtcaggggtc gggtgcagaa
     1261 tggccgcgag cagctccacg ccgtcgacga gtcgaggacc agggcggctg aagaaggatg
     1321 ccgcatcgac gacgtaaagg tccggccgct gttcgcaagc ccgccgccat gcgtcaccgg
     1381 tttgtccgac gcgcctaagc tcggtgactg tgcgctcaac ggagtacccg cagggcatta
     1441 caaggatcac atccggccga gcggcttcca cggcttgcca cgtcgtttca tgcgatgggg
     1501 catcctgaga cccaaggaca ttgcgtccgc cagccagctc gaccatttcc gggacccaat
     1561 ggcctgcaac atagagaggg tcgagccatt caaggcacac cacgcgcggg cgcgatgcag
     1621 tcctgtcggt ttccgcacgc acgcgtgtca ctcggcggcg gagctcctgg gtgagtgcca
     1681 gtcccttcgc gggtacgcct gccgcctcgg cgatgcgctc gatatcatgg atcatgtcgt
     1741 ctagcatcgt cgggctgagt gtgaggacct cagggcgatg ctgaagtgac gcgatcgctc
     1801 gcgtgagttg gtcaggtgtg accgagcaga cgtgacagag gtcttgcgtc agaataaggt
     1861 ccggactggc tcggcgaaaa gcgtcctcat tgagtcggta tagccgctgt cctgatgcaa
     1921 ccagttcttt gacttggctg tcgatttctt taccggatcc tccgtctttt ctgactaggg
     1981 gttcgatcat gaccggaaca tgtcgaaccg aggcaggaaa gtcgcattcg tggctgatgc
     2041 cgaccagctg gtcggccaaa tcgagggcgg cgaccacttc tgttgcaccg gggatgagcg
     2101 agcagattct catctgttat atcgcttctg tcggccacaa gcgggcatgc aaatctttca
     2161 ggcgcgtttc gcccaaacta tagctgattg catcggcctc gtgaaggtcc tgcacggtat
     2221 gcgtattggc taccgccagg actttcattc ccgctgatcg tgcggcccgg atgccggggc
     2281 gggaatcttc gatagccaag caggcatcag gcgggatcgg agcagcggaa tgccgacggt
     2341 tcaatccagc cagggcctgc aggaacggtt ccggattcgg tttaccgcac gtcacatcct
     2401 ctgcgctggt aatgtgacga aacgcttttc ggatcccgat ttgttccagc accaactcga
     2461 tctccgcgcg caaggcaccc gaggcgatgg caatggggca agtggccgcg gcgttttcga
     2521 caaattctct gacacctgga aaaatcacga ggtgatcctt gatcgaggtc aaatagacgg
     2581 cggacttttt cgccatcagc tcttcgacga gcgagggaga gatcgggcgc cgattgattc
     2641 gtagtgcttc ctgaatacag ccacgatcat cgaagccgag atagtcggcg taatagtctg
     2701 tttcagtcaa aacgatctcg atgcctgcca gtgtcctgcg cagactttca aagtggagcg
     2761 gctcggtgtc ggcgatgaca ccgtcgaagt cgaagatcac ggcgcgcatg gattcctgag
     2821 ggtcgtgatg tctcacccct aatgatgggg gcgcaaactg gcggcattat agcacagcgg
     2881 gcggcaatcc gatcgccgcc cgctgtgcct gatgagtgcg
//
LOCUS       DATMTI010000549         1777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_20834, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000549 DATMTI010000000
VERSION     DATMTI010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20834"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VN039_08790"
     CDS             complement(<1..1001)
                     /locus_tag="VN039_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HWV46098.1"
                     /translation="MATTRIPVRDLQIGMYVARLDLSWFRSPLLRHSFLIEHPSQIDK
                     LVRAGVKVVDIDLDRGIASEPHQVSDITHSATEAAPTWNIKKQSKPLAQLNEEYVQAK
                     LAKQQMDQAVQSVFATITKTGTVNPQQAVEAVQEITIAMRTLTDSAIFMALSQNRAGD
                     STLSRHALATCTLSLVLGQAFQFNPLELQELATAALLHDIGLLQLRPAVVRQAHATSG
                     LSKTDQHELETHPRRAVLMLERQGRIESAILHLIANHHVYLDDSGYPKESRGQFTSDR
                     TRILMVVDRYDELITGFGGTPPLTPHHTFQCLYQEARQGKIDQRILSSFIARVGIYPV
                     "
BASE COUNT          406 a          402 c          560 g          409 t
ORIGIN      
        1 accggataga taccgactct cgcgatgaat gacgacagga ttcgttgatc gatcttgcct
       61 tgccgcgctt cttgatacaa gcattgaaat gtgtggtggg gagtaagcgg aggtgtcccg
      121 ccgaatccgg tgatcaattc atcgtatcga tccacgacca tcaggatgcg ggtgcggtcg
      181 gaggtaaact gtccccgaga ctctttcggg tagccgctgt catcgaggta gacatggtga
      241 ttcgcgatca gatggagtat ggcgctctca atcctccctt gccgttccag cataagaacc
      301 gcacggcggg gatgagtttc cagttcgtgt tggtcggtct tggaaaggcc cgatgtggcg
      361 tgagcttggc ggacaacggc aggccggagc tgtaagagcc cgatgtcatg taaaagcgcg
      421 gcagtcgcaa gctcctgcag ttccaagggg ttgaattgga aggcctggcc taagaccagt
      481 gacagcgtac aggtggccag tgcatggcgg ctgagagtgg aatcgccggc ccggttttga
      541 ctcaatgcca taaaaatagc ggagtcggtg agggttctca tagcgatcgt gatttcttga
      601 accgcctcga ccgcctgctg aggattgacc gtccctgttt tggtgatggt ggcaaaaacg
      661 gattgtacgg cttgatccat ctgttgcttg gccagtttcg cttggacata ttcttcgttc
      721 aactgagcca aaggcttgga ttgtttctta atattccacg taggtgctgc ctcggtcgca
      781 gaatgcgtga tatccgatac ctgatgcggc tcagaagcga ttccccggtc gaggtcgatg
      841 tcgaccacct tcacccctgc gcgtactaat ttgtcgatct gcgaaggatg ttcaatcaga
      901 aatgaatggc ggagtaaggg agagcgaaac cacgaaaggt ccagtcgggc gacgtacatt
      961 ccgatctgaa gatcacggac ggggatgcga gttgtcgcca tcggaggtcc ggattgcacg
     1021 tgaccagggt tgcagaaata gctccctcag gtatcggcac gaacggggga taactgtagc
     1081 tgagcgggtg atgcggtgag tggtcaggcc gagtgatgag gcgggtgaag gaatgagaga
     1141 gagacctcca tgcgaggatc cgctttgtcg acttgtttgt acagatgggt ttcgatgatg
     1201 cgattgtcgt ttaagcccag tccttgacag atcgcatctt gggcgatctt tagaccacca
     1261 tcgaggtcgc gtcgtaaggc ggaagcaaaa aagaatcgaa tagtgagcgc gagcggtcca
     1321 gattgaagac gatcccgcag ggagaatcta gaagaagact gggccaatgc cagccatact
     1381 tgctggccga cgtatgtttt ataggcacgt ccggcagccg atagcaaacg acgtccattg
     1441 actgtcgcat attgatggtt gatgctggga gggacgggca gtgtaatctt gaatgtatcc
     1501 gatgtggcga ccgagggatg cgatagtatg caaagagcgg tcttggagtt cgaaggatca
     1561 gattgtgacg gcagagccgt accgttgatc cgtggtagcc ttttcggtgt ggattggcag
     1621 atcggatccc cacgacgcaa tgttgtggaa aggcgaaatc tggatgaacg gtaattgtat
     1681 tgacggcggt cggacggcat ggagccgaac taaaggaggt tcaggatctt tgccatattc
     1741 tgctcaaagc gatcttccgt gcgggcaaga taccgac
//
LOCUS       DATMTI010000550         1933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_105132, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000550 DATMTI010000000
VERSION     DATMTI010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105132"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VN039_08795"
     CDS             complement(<1..790)
                     /locus_tag="VN039_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HWV46099.1"
                     /translation="MILLLNLWNLPSPVLEEQVIFSDFMAKLENGDVVKVTINGHHVS
                     GVLKDNSKLRTYSADYPDLVQVLREKQVQIEAKPPDESPWYISFLVTWGPFVLFLGLF
                     VFFMRRMQTGDAALSLVKSKARMLTDDRKKVMFSDVAGIDEVKEEVQETVEFLKDPRK
                     FQKLGGRIPKGVLVVGPPGTGKTLLAKAIAGEAGVPFFSISGSDFVELFVGVGASRVR
                     DMFEQAKKHAPCIIFIDEIDAVGRSRGAGLGGGNDEREQTLNQLL"
     gene            complement(1006..1833)
                     /gene="amoC"
                     /locus_tag="VN039_08800"
     CDS             complement(1006..1833)
                     /gene="amoC"
                     /locus_tag="VN039_08800"
                     /EC_number="1.14.99.39"
                     /inference="COORDINATES: protein motif:HMM:NF016767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial ammonia monooxygenase, subunit AmoC"
                     /protein_id="HWV46100.1"
                     /translation="MASSNQPNSDRGYDVSEWYDSKPVKIGWLAILGIGVFWVLYQRA
                     FGYSHGLDSMTPEFDSVWMGLWRFNILANAVFFAVTIGWIWMTRDRNLANLAPKLELK
                     RYFYWMGWLVCYMWGVYYAGSYTLEQDAAWHQVIIRDTSFTASHIVAFYGTFPLYITC
                     GVATYLYAQTRLPLYSQATSFALVAAVVGPMFILPNVGLNEWGHAFWFVDELFSAPLH
                     WGFVTLGWCGLFGAAGGVAAQIISRMSNLADVIWNNAPKGILDPFPTQVNPNAKAGY"
BASE COUNT          443 a          642 c          456 g          392 t
ORIGIN      
        1 ccagcaactg gttgagcgtt tgctcacgtt catcattccc gccgccaagc cccgctcccc
       61 tcgaccgtcc gacggcatcg atttcatcga tgaaaatgat acagggcgca tgtttttttg
      121 cctgctcgaa catatcccgc acgcgagagg ccccgacgcc cacgaacagt tcgacaaaat
      181 cagacccgct gatgctgaag aaaggcacgc ccgcctcacc ggcgatcgcc ttggccaaca
      241 atgtcttccc ggttcccggc ggccccacga ccaacacccc cttgggaatg cgtccgccga
      301 gtttctgaaa cttgcgcggg tccttcagaa actcaaccgt ttcctgtacc tcctctttca
      361 cttcatcaat tcccgccacg tcggaaaaca tcaccttttt ccggtcatcg gtcagcatgc
      421 gggccttgct cttcacgagg gacagggccg catccccagt ctgcatccgc cgcatgaaga
      481 acacaaacag gccgagaaag agcacgaacg gcccccaggt cacgagaaaa ctaatatacc
      541 aagggctttc atcgggtggt ttggcctcga tctgaacttg cttctctcgc agcacctgga
      601 cgagatcagg atagtccgcc gagtaagttc gaagcttgga gttatccttc aacacgccgc
      661 tgacatgatg tccattgatg gtcaccttta cgacatcgcc attctcgagc ttcgccatga
      721 aatcgctaaa aatgacctgc tcctctaata caggggacgg caggttccat aggttcaaaa
      781 gcagtatcat gaacaggcct aacgacatcc aaaacagtat ttttttggtt ttgatattca
      841 agggtagcct cttacgatga gtccgtagcg tcagatagcc tatctacggg aagctctttc
      901 cccggagtgc agtcggcggc ggaggcccgg cgctctgtac ctcggagcgc cgaacccctg
      961 atcccacccc aactgctaat tatttgggct gctcgccctt tctgcttagt accctgcctt
     1021 ggcgttgggg ttcacctggg tggggaacgg atccagaatg cctttcggcg cgttgttcca
     1081 gatcacgtcc gccagattcg acatccggct gatgatctgc gccgccacac cccccgccgc
     1141 cccgaacaac ccgcaccacc ccagcgtcac gaagccccag tgcaacggcg ccgagaacaa
     1201 ctcatccaca aaccagaacg catggcccca ctcgttcaac cccacgttcg gcagaatgaa
     1261 catcggcccc accaccgccg ccactaaggc aaatgaggtg gcctggctgt acaatggcag
     1321 ccgtgtctgc gcatacaggt aggtcgccac gccgcacgtg atgtacagcg ggaacgtccc
     1381 gtagaacgcc acgatgtggc tcgccgtgaa gctcgtgtcc cggatgatca cttggtgcca
     1441 cgccgcatcc tgctccaacg tgtagctccc cgcgtagtac acgccccaca tgtagcacac
     1501 cagccagccc atccaataaa agtaccgctt cagctccagt ttaggggcca aattggccaa
     1561 gttccgatcc cgcgtcatcc agatccaccc gatcgtcacg gcaaagaaca ccgcgttcgc
     1621 cagaatgtta aatcgccaca gccccatcca cacggagtcg aattcagggg tcatggagtc
     1681 taacccgtgc gagtacccaa acgcccgctg gtacagcacc caaaacaccc cgatccccaa
     1741 aatcgctaac cagccgatct tcaccggctt cgagtcatac cactcagaaa cgtcataccc
     1801 ccgatcactg tttggttgat ttgaggacgc catgattcag acctccctag tgagattgat
     1861 gaacactctt cttccagctc cgattgttca gcctgcataa cggtaggcgg ccgttaaagc
     1921 tccgaaggct aac
//
LOCUS       DATMTI010000551         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_105133, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000551 DATMTI010000000
VERSION     DATMTI010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105133"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="VN039_08805"
     CDS             complement(<1..1403)
                     /locus_tag="VN039_08805"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HWV46101.1"
                     /translation="MPDWQKSWRLFIASLPLQALGIGMLSTLLSATLWSAAPATLTAL
                     EYTVYDTWSRHRAAARLSPSLTIVARDAASEKRFGKGPWDRSILAQLIVTIHEAGAAV
                     IGVDHRLDYASPAQLGGAASDALFLEALRAVSPIVLIHNIDSLLQSDAAIYGHITVAS
                     DLDHVSRHIPSSIRIGTRTVPAFGSVLSEVFHRRNAVPVRFSSGNSDADVLISIVGDG
                     SLAALQPIPLSSVWNTVERHDEPQLASWFKDKVVVIRASPGGSETWLLPSGSMVDGIT
                     AHLHFLNALLNHERIDSFSWFGRFTVTLVLASLAAWSLLRFRGLMGLLLAGAVVVLYG
                     TLTVSMLNLVSLMMPIAGPLTASLFVFIGSTVWVNLTASQRLILLERDMIRIQRESAA
                     VREALVLHEDRAEALQEDLETANEAIAFSAGRQEELTRTTTLLRHQIAEAQSQEQDAR
                     RHLGELEQQLGDLRAAST"
     gene            complement(1408..>2140)
                     /locus_tag="VN039_08810"
     CDS             complement(1408..>2140)
                     /locus_tag="VN039_08810"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV46102.1"
                     /translation="SYRRTLDLHPHFPDAHYRLALLLRIAGRTREAVQHLETAAAGGV
                     SQARLFLGNAYQTGQGVEKNLGLAIFWWMQAADLGQQTAADSLSKMRRQALSSESTKQ
                     RRIELQSAFQIYRNTLWNNFPDISRPTQQQSLGKVLLEQNPVEDAVPVLLKECFALSE
                     EAHAELAALYETGWERSLKPYDKNILACFESTSADGFAPAKKILARIYAKGLGVDADI
                     PKAKTLLKGLPKQETQSLIEELRLP"
BASE COUNT          474 a          587 c          640 g          439 t
ORIGIN      
        1 gtgcttgccg cccggagatc gccgagttgt tgttccagtt ccccaaggtg tcgacgagca
       61 tcttgttcct gactctgcgc ttcagcgatt tggtgtcgca gtaaggttgt cgttcttgtc
      121 aactcttcct gtcggccggc cgaaaaagct attgcctcgt tcgctgtttc caaatcttct
      181 tgaagggctt ccgctcggtc ctcatgcaga acaagcgcct ctcttacggc agcagactct
      241 cgctggatgc ggatcatgtc gcgttcaagc agaatcaggc gttggcttgc cgtcagattc
      301 acccaaacag tggagccgat aaagacaaac aatgaggctg tgagagggcc ggcgatcggc
      361 atcatgagag ataccaggtt caacatcgag acggtcagcg tcccatacaa aaccacgacg
      421 gcaccggcga gcaagagtcc catcaacccg cgaaaccgta agaggctcca agcagccagc
      481 gaggccagca ccagggtaac ggtgaagcga ccgaaccagc tgaacgagtc gatccgttca
      541 tggttgagta aggcattcag gaaatggagg tgggcggtta tgccgtccac catcgagccg
      601 gacggtagga gccatgtctc cgagcctccg ggacttgctc ggatcacgac gactttgtcc
      661 ttgaaccaag aagccaactg cggctcgtcg tgacgttcga ctgtgttcca gacggatgac
      721 aatgggatcg gctgaagggc tgccaacgat ccatcgccga ctatgctgat cagtacgtcg
      781 gcatcggagt tgccggaaga gaaccgtacg ggcacggcgt ttcgtcggtg aaagacctcg
      841 gacaggaccg acccaaaagc tggaaccgtt cgagttccta tcctgatgga cgacggaatg
      901 tggcgggata cgtgatcgag atcgctcgcg accgtgatgt gtccatagat cgctgcatcg
      961 gactgaagga gtgaatcgat attgtgaatc aggacaatgg gtgataccgc acgaagcgct
     1021 tcaaggaaga gcgcatcgct tgcagctccg ccaagctgag cagggctggc atagtccagt
     1081 cggtggtcaa cgccgatgac ggcggcaccg gcctcatgga tcgtcacgat caactgagcg
     1141 aggatgctgc ggtcccaagg accctttccg aatcgtttct cactggccgc gtcgcgagcc
     1201 acgatggtca gcgaggggct gagccgggcg gcagcacggt ggcgagacca ggtgtcatag
     1261 acggtatatt ccagcgccgt caaggtagcg ggggcagcag accatagcgt cgccgagagg
     1321 agcgtggaca gcatgccgat gcccagcgcc tgcaaaggaa gcgacgcgat gaacaggcgc
     1381 caggatttct gccagtccgg catacaacta tggaagcctt agctcctcga tcaaggattg
     1441 tgtttcttgt ttcgggaggc ctttgaggag agtctttgct ttcggaatgt cggcgtcgac
     1501 accgagcccc ttggcataga tacgagcaag gattttcttt gccggggcga agccgtcggc
     1561 cgaggtcgat tcaaaacatg ccagaatatt cttgtcgtac ggcttcagag accgttccca
     1621 tccggtttca tagagggcgg ccagttcagc atgggcctct tcactcaacg cgaagcattc
     1681 cttcaaaagt accggaaccg catcttccac ggggttttgt tcgagaagga ccttccccag
     1741 cgattgctgc tgagtcgggc gactgatatc gggaaagttg ttccaaaggg tgttgcggta
     1801 gatctgaaaa gcgctctgca attcgatgcg ccgttgcttc gtcgattcgg aagaaagcgc
     1861 ctggcgccgc atcttggaca acgaatcagc ggcggtttgt tgcccaaggt cggccgcttg
     1921 catccaccaa aaaatcgcca aaccgaggtt tttctccacg ccctgcccgg tctggtaggc
     1981 attgccgagg aacaatcgtg cctgagacac cccaccggcc gccgccgttt ccagatgttg
     2041 cacggcttct cgcgtgcgtc ccgcaatcct caataacagc gcgagtcgat agtgtgcgtc
     2101 ggggaaatga ggatgcagat caagtgtccg tcgataagat
//
LOCUS       DATMTI010000552         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_657336, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000552 DATMTI010000000
VERSION     DATMTI010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657336"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..334
                     /locus_tag="VN039_08815"
     CDS             167..334
                     /locus_tag="VN039_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46103.1"
                     /translation="MLVLIHKECGGPALEESSVGEVCAIPVDRFPFPCFTCLEEILDE
                     SEVRLSEELGI"
     gene            complement(419..>1507)
                     /locus_tag="VN039_08820"
     CDS             complement(419..>1507)
                     /locus_tag="VN039_08820"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV46104.1"
                     /translation="DFRGDTLHPSTLEIMHELGLLERFLRLPHQKVQRINARFGDLEF
                     TVADFSHLSTQCRYVAFMPQWDFLNFLVEVGSRYPGFQVRMSAEVTGVIESGGSVVGL
                     RAETATGPMDVRAALVVGADGRHSTVRKRAGLSVEEFGAPMDVLWFGLSRNPDDPLDP
                     MGRFDAGRIFIMLNRGDYWQCGFVIAKGSLDEIRARGLPLFRDSVAKLAPFVTDRVHE
                     LQDWEPIKLLTVQVDRLRQWYKPGLLCIGDAAHAMSPVGGVGINLAIQDAVASANLLW
                     HPLRDGLVTEADLAKVQARRMWPTRLTQRAQLIIQHRVIRRVLNSKVSLSPPWAVGLL
                     ARFPFLRRIPARLIGLGIRPEHVRTPAG"
BASE COUNT          327 a          457 c          414 g          309 t
ORIGIN      
        1 agcaagatac cgttatttcc ccggatggca gccggcggtt cgccaatgag ctgcttcggc
       61 gcgatcccag atttccgctt gatcttcaac tgattcccgg cgaccaccga ctcagtagtc
      121 cccaacacct tgagcggttt cgccgactgg tgatgaggga agcttgatgc tggtgctaat
      181 ccacaaagaa tgcggcggcc cggcattgga agaatcgtcg gtgggcgaag tctgcgcgat
      241 tcctgtcgat cgcttcccct ttccttgttt cacctgcctg gaagaaattc tcgacgaatc
      301 cgaagtgcgg ttgtcggaag agttggggat ctgacaggtt atagtcccta caatcaccgg
      361 tacattcttt ctattctcga ccgaccatct gactctgctc caaaagcagg acgctggttt
      421 agccggccgg cgttcgtaca tgttcaggcc ggatcccaag cccgatcaac ctggcgggga
      481 tacggcgaag aaaggggaac cgggccagca gcccgacggc ccaaggaggt gacagtgaaa
      541 ccttgctgtt cagcacacgt ctgatgacac gatgctggat gatgagttgc gctcgttgag
      601 tcagccgggt aggccacata cggcgggctt gcaccttggc caaatccgct tcggtcacca
      661 gcccatcgcg caatgggtgc cataacagat ttgccgacgc caccgcatcc tgaatcgcca
      721 ggttgatccc aactcctcca accggcgaca tggcgtgggc tgcgtctccg atacagagca
      781 gcccaggctt ataccattgc cgtaaccgat cgacttgcac ggtcagcagt ttaatcggtt
      841 cccaatcttg cagttcatgg acgcgatctg tgacaaacgg ggccaacttc gccacgctgt
      901 cgcggaacag cgggaggcct cgtgctcgta tctcgtcgag tgatcctttg gcaatgacga
      961 agccgcactg ccagtagtca ccccggttca gcatgatgaa gattcgaccg gcatcgaaac
     1021 ggcccatcgg atcgagcggg tcatcgggat tgcgtgacaa tccgaaccac agcacatcca
     1081 tgggagcacc gaattcttct accgaaagcc ccgctctctt gcggacagta gagtgtctgc
     1141 cgtcggctcc cacgacaagt gcggcgcgaa catccatcgg ccctgttgcc gtttcagcgc
     1201 gaagccctac gacggaccct ccgctctcga tcacgcccgt cacctccgcg ctcattcgga
     1261 cctgaaatcc aggataccgg cttcctacct cgacgagaaa gttgagaaag tcccactgcg
     1321 gcatgaaggc aacgtaccgg cattgggtcg acagatgaga gaaatccgcc accgtaaatt
     1381 ccagatctcc gaaccgcgcg ttgatccgct gtactttctg gtgaggcaat cgaaggaacc
     1441 gttcgagcag ccccaactcg tgcatgattt ccaacgtcga ggggtgcaag gtgtcgccgc
     1501 gaaaatc
//
LOCUS       DATMTI010000553         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_827673, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000553 DATMTI010000000
VERSION     DATMTI010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827673"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VN039_08825"
     CDS             <1..500
                     /locus_tag="VN039_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWV46105.1"
                     /translation="VTAKVGQQIVAQVSDRLLYQSHPDSVVGAIDQMLADAISRVQEL
                     KQSLTGVDAQIRELKIETVHQSLLSLQRDLAIRSGGIERLTVLRGAAAAGQPLSALPQ
                     YSSAYVAAVLRGPFLLVPTDDLLFRPGDIVVVIGAQTELNQLVSWFTGQRPLTPIAHK
                     IGVRE"
     gene            535..1926
                     /locus_tag="VN039_08830"
     CDS             535..1926
                     /locus_tag="VN039_08830"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWV46106.1"
                     /translation="MLLVAFPSSRVLLCVVAILLSAAIGSDRAEAFSTDSPGIRMLEE
                     IQTVITELAEQAKPSVVNLFPVPSAGKLREGPGERTPNASGSGSGLIVDREGHIVTNN
                     HVIGDATEIEVRFSDKTKLIAHVIGKDPDTDLAVLKVTTDRPLPNARFGDSAGVKVGQ
                     WVLAVGNPFGLDRTVTLGVVSGIGRENINLSRYENFIQTDASINPGNSGGPLFNLRGE
                     VIGINTAIINFAQGIGFAIPSNMAKQVIEQLLAKGKVVRGWLGVGIQPLTAELAKKFG
                     VAEGEGVLVNEVFEKDPAALAGIKPGDVIVRIDGAVVDSPNKLSRLIATLTPGATSKI
                     EIVRDLKHVMMDVPLTERRDTTVTASIPQQEEKVGGRLGLDLQDLTAALAERFKLRES
                     KGVLIAKVEPNSLAQAEGLREGDLIKEVNRMDVSSVGEFTSAIARSRRGDTLLLRVLR
                     ENRAFYVVLKSSD"
     gene            complement(1932..>2238)
                     /gene="fusA"
                     /locus_tag="VN039_08835"
     CDS             complement(1932..>2238)
                     /gene="fusA"
                     /locus_tag="VN039_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWV46107.1"
                     /translation="LLEPVMTVEIDTPTDAVGAVMGDLSARRGRILTVNAQDHAEQIK
                     ALVPLAELLRYATTLNAMTGGRGSYVMDFAQYDEVPRELAARVIERHKTEGQAATAH"
BASE COUNT          472 a          640 c          622 g          504 t
ORIGIN      
        1 atgtgacggc caaggtcggc caacaaatcg tcgcgcaggt ttctgatcga cttctgtacc
       61 agagtcatcc cgattcagtt gtcggtgcga tcgatcaaat gcttgccgat gccatctctc
      121 gcgttcaaga gttgaagcaa tcgttgaccg gggttgatgc ccagattcgt gagctcaaaa
      181 tcgagacggt ccatcagagt ttgctctccc tccaacgtga tttggcgatt cgttctgggg
      241 gtattgaacg gttgaccgtc cttcgtggcg ctgccgcagc tggacaacca ctcagcgcgc
      301 ttccacagta ttcctcggca tacgtggccg cggtattacg aggacctttt ctgctggtcc
      361 cgaccgatga tctcctgttc cgccccggcg acatcgtggt cgtaatcggt gcccaaacgg
      421 aactcaatca actcgtctca tggtttaccg gccaacgtcc cctgacaccg attgctcata
      481 agatcggcgt aagagagtag gcacatcgac ggagatcaga tacaataacg ctcgatgtta
      541 ctcgtcgcat tcccctcatc acgtgttctg ctgtgtgttg ttgctatcct tctatccgcc
      601 gccataggaa gcgaccgagc cgaggcattc tcgaccgact cacccggcat tcggatgctc
      661 gaagagatcc aaaccgtcat tactgaactc gctgagcagg ccaagccttc tgtcgtcaac
      721 ctgtttcccg tccccagcgc gggtaagctt cgcgaaggac ccggcgagcg tacgccgaat
      781 gcgtccggct ccggttctgg cctgatcgtc gaccgtgaag gtcatatcgt cacgaacaac
      841 catgtgatcg gcgatgcgac tgaaattgaa gtgcggttct ccgataagac gaagcttatc
      901 gctcatgtga tcggcaaaga tccggacacc gatttagctg tactcaaagt gaccaccgat
      961 cgaccgctgc ccaatgctcg attcggtgat tccgccggcg tcaaggtcgg gcaatgggtg
     1021 ctggccgtcg ggaatccgtt cgggttggac cggaccgtga cgcttggggt ggtcagcggc
     1081 atcggtcgag agaacatcaa cctttcacgg tatgaaaact tcattcagac tgatgcctcg
     1141 atcaaccctg gaaattccgg aggcccgctc tttaacctcc ggggtgaggt gatcggcatc
     1201 aatacggcaa tcatcaattt tgcccaaggc atcggattcg ccattccctc gaacatggcc
     1261 aagcaggtca tcgaacagtt actggccaag ggtaaggtcg tacggggctg gctcggcgtc
     1321 gggattcagc cgttgaccgc tgagttggcg aaaaaattcg gagtggccga aggcgaagga
     1381 gtactcgtga atgaagtgtt cgagaaagat cctgcagcct tggccgggat caagcccggc
     1441 gatgtcatcg tgcggatcga cggtgccgtc gttgattcac cgaataagct gtctcgtctc
     1501 atcgcgacac tgacgccggg cgcgacctca aagatagaaa ttgttcggga tctgaaacac
     1561 gtgatgatgg acgttcccct caccgagcgt cgcgatacca ccgttaccgc atcgattcct
     1621 caacaagagg agaaggtggg gggcagactg ggtctcgacc tccaagacct gacggccgcc
     1681 ctagcggaga ggttcaagct ccgtgagtcg aaaggcgtgc tgatcgcaaa ggttgaaccc
     1741 aacagtcttg ctcaagccga aggtctccgg gaaggcgacc tcataaaaga ggtcaaccgc
     1801 atggatgtgt cctccgtcgg agaatttacg tcagccattg cgcggtccag acgcggtgac
     1861 acactgctgc tccgcgtgct tcgggaaaac cgtgcctttt atgtcgtgct caaatcctcc
     1921 gactgacgat ttcagtgtgc cgtggcggct tgtccttctg tcttatgccg ctcgatgacc
     1981 cttgcagcta actctcgtgg cacttcatca tactgtgcga aatccatgac atagctaccc
     2041 cgtcctccgg tcatggcgtt gagggtcgtc gcatatcgaa gcagttcagc cagcggcacc
     2101 agcgctttga tctgttcggc atggtcttgc gcgttaacgg tcaggattcg accccgtcgg
     2161 gcgctcaagt ctcccatgac agctccgacc gcatcggtcg gtgtgtcgat ttcgaccgtc
     2221 atgacaggct caagtaac
//
LOCUS       DATMTI010000554         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_148016, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000554 DATMTI010000000
VERSION     DATMTI010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148016"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VN039_08840"
     CDS             <1..212
                     /locus_tag="VN039_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46108.1"
                     /translation="RIVRQHGNVRRHLTLLPYLRAWQRLCRSLQERRETAGERGGKAL
                     LDQLYSPQLQVHSLGRHEEIQIVQG"
     gene            213..1349
                     /gene="waaF"
                     /locus_tag="VN039_08845"
     CDS             213..1349
                     /gene="waaF"
                     /locus_tag="VN039_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWV46109.1"
                     /translation="MIRVLPSSPISDERGLLEGCPVPFISPLDSRSASALENARILLI
                     KPSSLGDIVHAFPVVSAIKTQWPGSHITWLVKRQWADLVERAEGVDRVWPVEMRIRSW
                     IREGRALRAQRFDIAIDLQGLFRSAIVARFSGAPTRIGFANGREGSPWFYTQRIPVLS
                     PDIHAVDRYLSVAAALGSSLPGKPRFGLRLLEKDMATVRELCRRKGFSMDKPWVAMNI
                     GARWPTKRWPLASFAAVVDQLHETHRDPVVMIGSADERAYTNRLRALTDRPFVDLSGE
                     IPLGCLPALLSTATAMITNDSGPMHIAAALGVPVIAMFGPTSAIRTGPYGAGHHVLAG
                     RVSCSPCFSRVCRHDPELECLHLIKPTQVVDVIRPLLAAHVPCR"
     gene            1340..2365
                     /locus_tag="VN039_08850"
     CDS             1340..2365
                     /locus_tag="VN039_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWV46110.1"
                     /translation="MSMNVLIVRPDGIGDVLLSLPVATQLKRLVPGVTVGFLTSPTVA
                     PLLDRHPDVDYVRAIRFTDPFKKLRLAFSQRVEAAIFLKPFRRLMWAAWVAGVPIRVA
                     TRYRWYSPLANRHIYEHRSEFSKHESEYNVEMLKGLGLRPQPTRPPVLTLTEAERAAG
                     ASQWSGLPRPRVVIHPGGVSARRWRLEQYRDLVVTLTDRGYGVVLTGSDQERTEFGKS
                     LPMSTALPAGVVDLMGHLSLRELMSVIANAHVVVSGATGPAHLAAALGIPTLTLFDPR
                     RNNLPVRWKPLGLGALLRPDVPTCEKCIGEACPYWDCLDRFTVEKVIATIDKIVENPS
                     PLTVLHI"
BASE COUNT          506 a          705 c          740 g          595 t
ORIGIN      
        1 ctcgaatcgt acgtcagcac ggcaacgtgc gccgccactt gaccctgctg ccgtatctac
       61 gggcttggca gcgcctctgc cgatcacttc aagagaggcg cgagacggcg ggcgagcggg
      121 gcggcaaggc actgctcgac cagctctatt ctcctcagct gcaggttcat tccctagggc
      181 gccatgaaga gattcagatc gtacaaggat gaatgatacg cgttctacct tcgtctccaa
      241 tttctgacga gcggggcttg ctggaaggtt gtccggtgcc gtttatcagc cctctcgata
      301 gccgttccgc ctcggcgttg gagaacgccc gtatcctttt gatcaaaccc agttctcttg
      361 gagatatcgt tcatgcgttt ccagtcgtct cggcgatcaa aacacaatgg ccgggatcgc
      421 atattacgtg gctggtgaag cgccaatggg ctgaccttgt cgagcgggcg gaaggagtcg
      481 accgtgtctg gcccgtagag atgaggataa ggagttggat cagagaaggc cgggcgttgc
      541 gtgcacagcg gttcgacatc gcgatagatt tgcaagggct gtttcgcagt gccatcgtgg
      601 cacgcttcag cggcgcaccg acgcgtatcg gattcgcgaa tgggagagaa ggaagcccat
      661 ggttctatac tcaacgtata ccggttctca gccctgacat acacgccgtc gaccgatatc
      721 tttcagtggc cgcggcgttg ggatcatctt tgccgggaaa accgcgattt gggctcaggt
      781 tactagagaa ggacatggcg accgttcgag agctctgccg acgcaaaggc ttttcaatgg
      841 ataagccatg ggtggcgatg aacattggcg cacgctggcc gacgaaacgc tggccactgg
      901 cgtctttcgc cgccgttgtg gatcagctgc acgaaacaca tcgtgatccg gtcgtcatga
      961 taggaagtgc ggatgagcga gcatacacga ataggttgag ggctctgacg gaccgtccgt
     1021 tcgtcgatct gagtggtgaa atcccattag ggtgcttgcc cgccctcctc tcgaccgcaa
     1081 ccgccatgat cacaaacgat tccggaccga tgcacattgc tgccgcgctt ggagttccgg
     1141 tcatagcgat gtttggaccg accagcgcga ttcggaccgg tccgtatggg gccggacatc
     1201 atgtcttggc cggtcgagtg tcctgtagcc cttgcttcag tcgtgtctgc cgacacgatc
     1261 ctgaattgga atgccttcat ctcattaaac cgacccaagt ggtcgatgtt atccggccgc
     1321 tattggcggc tcatgtacca tgtcgatgaa tgttctgatc gttcgcccgg acggcatcgg
     1381 cgacgtcctg ctctcccttc ccgtggcgac tcaactcaag cggcttgtgc ccggggtcac
     1441 agtcgggttt ctcaccagtc ccaccgtcgc accccttctc gatcgccatc ccgacgtgga
     1501 ctatgtgcga gcgattcgct tcacagatcc attcaaaaaa ctccgtctcg ccttttcaca
     1561 gagggttgag gcggcgattt ttctgaagcc gtttcgtcgg cttatgtggg ccgcatgggt
     1621 ggccggtgtg ccgattcgag tggcaacgcg gtaccgttgg tatagcccgt tggccaatcg
     1681 ccatatctat gaacaccgca gtgaattttc gaaacatgaa tcagagtaca acgtcgaaat
     1741 gttgaagggc ttggggttga ggccgcagcc tacacgcccg ccggtgctca ctctgaccga
     1801 ggctgaacgc gccgccggtg cgtcccaatg gtcgggattg ccgcgtcctc gcgtcgtcat
     1861 tcacccggga ggagtttctg ctcgccgctg gcggctggag cagtaccgtg atctggtcgt
     1921 gacgttgaca gacagaggct atggggtcgt gcttacaggc agcgatcagg aacgaacgga
     1981 gttcggaaaa agtttaccga tgtctacggc gcttcccgca ggggtagtgg atcttatggg
     2041 tcatctgtcg ctgcgagaac tgatgtcggt gatcgccaat gctcatgtcg tcgtctcagg
     2101 agcgacagga ccggctcacc tggcggcagc cctgggtatc cccaccctca ccttgtttga
     2161 tcctcgacga aacaatctgc cggtgagatg gaaaccgctt gggctgggag cattgctgcg
     2221 ccccgacgtt cccacttgtg aaaaatgtat tggagaagcc tgtccttatt gggattgtct
     2281 tgatcggttc actgtcgaga aggtgatcgc cacaatcgac aagatcgtgg agaatccttc
     2341 tccactcacc gttcttcaca tataagtcag ggatcaagga taactctcct ggtccactct
     2401 tgactccaat tcgcttctgt gttcatattc tgaacgtttg gttctaacca tatgaatctc
     2461 caaggacaac gagatcttct cctgctgacc gaggtagagc gagatggtac ggttactcag
     2521 cggtctctcg cttcaaagtt aggggt
//
LOCUS       DATMTI010000555         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_699124, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000555 DATMTI010000000
VERSION     DATMTI010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699124"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1521)
                     /locus_tag="VN039_08855"
     CDS             complement(<1..>1521)
                     /locus_tag="VN039_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46111.1"
                     /translation="FEDWCEEYLDTKLFNHHLQWVDLLEGREPRNLHENQTYIPGEPE
                     FLLINTPPEHAKSTTITMNYVTYRICQDPNIRVIIVSQTQEMAKRFLRGIKDRLASEN
                     RNYQKLQVDFAPEGGFDDGAAAWTADSIYVSSATRDSGEKDPTVQALGIGGHIYGSRA
                     DLIILDDCVTGKNAHEFEKQMDWLQREVYNRLSYPGGRILLVGTRLAPIDLYGEIIKD
                     DYYGEESSPWTYLTQPAVLEFADDPKDWKTLWPRTNRPPVSVAGRQLVEQDEDGLWPM
                     WTGEALRKRRASMSPKNWALVYMQESVVEDAIFPMKAVTGCVDGMRAAGAMTPGAPGH
                     RTHGMNGLYVVGGFDPAMTGHSAAVILGVDRHSGERWILDLWSKGNLKPDDIFNKIKE
                     FTVKYGMNEWRIEKNAMNLMVTQNRDIKQFLGARGCLLREHFTGSNKWDADFGVASMS
                     VLFEGYENGSNLIHIPSRSQSEATKMLIEQLTTWEPLPPGVKTKKKTDLVMALWFA"
BASE COUNT          298 a          450 c          430 g          343 t
ORIGIN      
        1 tccgcgaacc acagggccat aacgaggtcg gtcttcttct tggtcttgac tcccggaggc
       61 agaggctccc aggttgtcag ctgctcgatg agcatcttgg tcgcctcgct ctgtgagcgg
      121 gacgggatgt ggatcaggtt gcttccgttc tcgtagccct cgaacaggac ggacatagag
      181 gcgacgccga agtcggcgtc ccacttgttc gatccggtga agtgctcacg aaggaggcag
      241 ccacgcgcac caaggaactg cttgatgtca cggttctggg tgaccatgag gttcatcgcg
      301 ttcttctcga tgcgccactc attcatcccg tacttgaccg tgaactcctt gatcttgttg
      361 aagatgtcgt caggcttcag gttccccttc gaccacaggt ccaggatcca cctctccccg
      421 gagtggcgat ccactcctag gatcacggca gccgagtggc ctgtcattgc cgggtcgaaa
      481 ccacccacga cgtacagccc attcatgcca tgcgtcctgt gaccaggggc accaggtgtc
      541 atcgcacctg cggctctcat cccatccaca catccagtta ccgccttcat agggaagatc
      601 gcgtcttcga cgacgctctc ctgcatgtac accagagccc agttcttcgg gctcatggag
      661 gcgcgacgct tacggagcgc ctctccggtc cacatgggcc agaggccatc ctcatcctgc
      721 tctacaagct gccgtccagc cacggagacc ggtggacggt tagtcctcgg ccaaagggtc
      781 ttccaatcct tggggtcatc agcgaactcc agaaccgctg gctgagtaag gtacgtccac
      841 ggagaagact cctcgccgta gtagtcgtcc ttgatgatct caccgtagag gtcgatggga
      901 gcaagacgag taccaaccag caggatgcgg ccaccagggt acgagagacg gttgtagacc
      961 tctcgctgga gccagtccat ctgcttctcg aactcatgag cattctttcc agtaacgcag
     1021 tcatcaagga tgatgaggtc tgctcgggat ccatagatgt gtccaccgat accgagagcc
     1081 tgaaccgtag ggtccttctc accggagtct cgggttgcgg agcttacgta aatggagtcg
     1141 gcggtccagg ccgccgctcc atcatcgaat ccgccctcag gggcgaagtc gacctgaagc
     1201 ttctggtagt tcctgttctc cgacgccaga cggtccttaa taccacggag gaacctcttt
     1261 gccatttcct gagtctggga gacgataatg actcggatgt tcgggtcctg gcagattcgg
     1321 tacgtcacat agttcatcgt gatcgtggtg gacttggcgt gctctggcgg agtgttgatg
     1381 agaaggaact caggctcacc tgggatgtag gtctggttct cgtgcaggtt ccttggctct
     1441 ctaccctcca gcaggtcaac ccactgaaga tggtgattga agagcttggt gtcgaggtac
     1501 tcctcgcacc agtcttcgaa t
//
LOCUS       DATMTI010000556         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_530522, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000556 DATMTI010000000
VERSION     DATMTI010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530522"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VN039_08860"
     CDS             <1..551
                     /locus_tag="VN039_08860"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV46112.1"
                     /translation="LSEGMLAKAKEKAEAHSLDHVQLHRMDAGAMDFTDDSFDTVVAA
                     YVVTAVPDYRKVVNEMIRVCRPGGRIIMLNHFSNGNKVIAAVEKVISPLTKHLGWRTD
                     LSLNTVLEGTTLEIARKQRVNPLRFWALVECVNGKGKQTNGNAVTHPIVGYMNGSGTA
                     GFANGTNGNGNANGHYSAEHAH"
     gene            complement(574..1392)
                     /locus_tag="VN039_08865"
     CDS             complement(574..1392)
                     /locus_tag="VN039_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46113.1"
                     /translation="MKHSLKNADDLRWLIGHTGGFRLGYVTDVHMSKRRLFDEGSGRE
                     VPAGTTVSVTIRYEVRGMVRVAKLTMTGVTDFSVFEQEGVDCSTLTAIQTEFSAGKLR
                     FWFDPQGELYVVCEEAHLDEITTPFVTADQAAEFARWTFQGRTAEGPPVSWLLHELDR
                     AGLPCAWREAKRAVAIHPTVQWEGDLIPIGDSTVNGNHVVHVMAYGLWERQGFGLMLR
                     VFGIQDRRMSRILEVLADQITRHFAGDCLVGTTIIPGREWEGWLIREHHARHDR"
     gene            complement(1985..>2468)
                     /locus_tag="VN039_08870"
     CDS             complement(1985..>2468)
                     /locus_tag="VN039_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWV46114.1"
                     /translation="HKKVPASKVREKPTVSPCLSKVPRHRLVELLWSFTPAYQRWSES
                     LLVEKGLSPQRLRILGSIHERGPRIMSELKEELGVTATNVTALVDSLEKDGLVKRKQH
                     PTDRRATVIELTDKVMTELSPRCTEYKEQVAELFSDLSERECKAFVTTLEKLWRRLQK
                     "
BASE COUNT          555 a          707 c          683 g          523 t
ORIGIN      
        1 atctgtccga aggcatgctg gcaaaagcta aggaaaaggc cgaagcccac agccttgatc
       61 atgtccagct tcatcgcatg gacgcaggtg ccatggactt cacggatgac agtttcgata
      121 cagtggttgc agcctatgtg gtgacggcgg ttcccgacta tcgcaaagtc gtcaatgaga
      181 tgattcgagt ctgtcgtccg ggtggccgca tcatcatgct gaatcacttc agcaacggca
      241 ataaggtcat tgccgccgtg gaaaaagtga tttctccgct gaccaaacac ctcggttggc
      301 gaacagactt gtcgctcaat accgttttgg agggaacgac tctggagatt gcccgaaaac
      361 agcgggtcaa cccattacgg ttttgggccc tggtggaatg tgttaacggc aagggcaaac
      421 agaccaacgg aaatgccgtg actcacccca tcgtggggta tatgaacggg agcggtacag
      481 ccggttttgc aaacgggaca aacggaaacg gcaacgccaa cggacactat tctgctgagc
      541 acgcgcactg atcagtttca cctttcattc ctcctatcga tcgtgtcgtg catgatgctc
      601 ccgaatcaac cagccctccc attcacggcc tgggatgatc gtcgtaccga cgaggcagtc
      661 acctgcaaaa tgtcgggtga tctgatcggc caggacttcg aggatccggc tcattcgccg
      721 atcttgaatc ccaaacaccc gcagcatcaa accgaaacct tgcctctccc ataggccata
      781 ggccatgaca tggaccacgt gattcccatt gaccgtggag tcgcctattg gaatcagatc
      841 gccctcccat tgcaccgtcg gatgaatcgc gacggcccgc ttcgcttcac gccaagcaca
      901 aggcaggccg gctcgatcca attcgtgtaa cagccacgac actgggggac cttcggcagt
      961 tcgaccttgg aacgtccatc gagcaaattc ggcggcctgg tcggccgtca caaacggcgt
     1021 ggtgatttca tccagatggg cttcctcgca caccacgtag agctctccct ggggatcaaa
     1081 ccagaagcga agttttcctg cgctgaattc cgtctgaatg gcggtaaggg tggaacaatc
     1141 gacaccttct tgctcaaaaa ccgaaaaatc cgtcacgccc gtcatggtga gtttggccac
     1201 tcgtaccatc ccacgaacct cataccggat ggtgaccgag acggtagtac cggccggcac
     1261 ctcccgtccg gaaccttcat cgaagaggcg gcgtttggac atatgcacgt cggtcacata
     1321 tcctaagcgg aagccgccgg tatgcccgat cagccaccga agatcatccg cgttcttgag
     1381 ggagtgtttc acgacaccaa ttctagcgca tggacggact cacggccagg cttgcgcgag
     1441 catccaacat gatgccaccg cgggaagccg ggagcgcagc gcggccttga gcgcacacga
     1501 gccgtgccgt gcgaggccga ccttctcaca ccaggcagct gcgatagctg cctcctttct
     1561 gccttggttg atcatgatga acaggccgtg gggttttagg ttccgggcga cctgcgagaa
     1621 cactgtgcgc ggtgccaaca cggaaagcgg catccgccaa gccagcacag gatcgggcgt
     1681 cacgaaggga taccatgccg tcacgacgtc agctggacga ttatagcagg catcgtcgcc
     1741 gacgaagaac tccgtgttcg gtaggtcttg gatataaccg ttcgcataat cgagagaatg
     1801 ttcggattct tccggagcca ggagccaagg tgatcgttct ttcgttcaat ccagacgagt
     1861 ccatcagcgt aagagacata catctggccg gtgccataac gcttcgtatc tgaacgtcag
     1921 ggataccatg ccgtcgatca cgtcacctgt tatcgctaag aatgtggatc ccgataggaa
     1981 gggtttactt ctgcaatcgg cgccagagct tctcaagcgt ggttacaaag gctttgcatt
     2041 cgcgctcgct gagatcggag aacagctcag cgacctgttc cttgtactcc gtgcagcgcg
     2101 gggaaagttc ggtcatgact ttgtcggtga gctcgatgac agtcgcgcgg cgatcggtcg
     2161 ggtgctgttt ccgtttgacc aatccgtctt tttcgagcga gtcgacgagc gccgttacat
     2221 tcgtcgcggt cacaccgagt tcttctttga gctcgctcat gatccgtggg ccgcgttcat
     2281 gaatcgagcc gagaatgcgc agccgctgag gagacaaccc tttttcgacc aataaagatt
     2341 ccgaccatcg ctgataggcc ggggtaaaac tccataacag ttcgaccagt cggtgccgcg
     2401 gcactttgga gaggcaggga gacacggtcg gtttctccct gaccttcgat gccgggactt
     2461 tcttgtga
//
LOCUS       DATMTI010000557         4484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_360793, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000557 DATMTI010000000
VERSION     DATMTI010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360793"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1389)
                     /locus_tag="VN039_08875"
     CDS             complement(145..1389)
                     /locus_tag="VN039_08875"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HWV46115.1"
                     /translation="MPSKEIPIVSQSAAAQNDSDDEPTFPPYTGPAFLSYGFRPFFLG
                     AALFGSLAILVWVVLFAGGVYAAFLYSPREWHVHEMLFGFIPALIAGFLLTAMPNWTD
                     RMPLRGMPLLVLFLLWLAGRLLVVAPWFGSAFSAVVDGAFLVLLAAYVWREIIAAGCW
                     DRAPIGMLVGFYACANIVFHVSALRGAPTDFPERFALAVMTLMLTIIGGRLTPTFTRE
                     FLAEKNMPRLPEIFSPIDGIAIILVLAGVITWVFQPESGWAGTMLLIAGAASMARLLS
                     WGGWRTWREPLVLILHVGYGWVGLFLIALGVAILGIGFSTENAVHLLTTGAMGAMTLA
                     VMTRASLGHTGRTRHADRLTVAIYLLVNVGALLRIFAPHSETPTTLTHTILGLSALCW
                     GGAYVLFALHYGPYLVRPSLDD"
     gene            1798..2109
                     /locus_tag="VN039_08880"
     CDS             1798..2109
                     /locus_tag="VN039_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46116.1"
                     /translation="MTCTRCSGLMVADHLLDMQESYLPMWMPALRCLACGNIVDSLIH
                     YHRAVRRTRRATRLAAQLAKRSTVRSNPAGGGVVGLSAVVSAGPSVKLKPATDQGLPV
                     I"
     gene            complement(2160..4010)
                     /locus_tag="VN039_08885"
     CDS             complement(2160..4010)
                     /locus_tag="VN039_08885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HWV46117.1"
                     /translation="MDNLTRQLNERFGFSTFRPGQEAVIRAVLAGRDAMAVMPTGQGK
                     SLCYQLPATLLPGLTLVISPLIALMQDQVTAMRQRKIAAAAFHSGLSGLEKSRVMQDL
                     QQRRVQLLYLAPERMQHEGFLRLLRSLWVSLLVVDEAHCISQWGHDFRPDYLKIGRLR
                     RELTNPPCLALTATATARVQTDLCQRLSLRDPFRLVTGFRRANLALSVHHCQSLREKL
                     GMVERLVCGTEKGTILIYCATRRTVEEVAGWLGQSHQAVGYYHAGLSDEQRRLVHDDF
                     RRGTLRILVATNAFGMGIDKPDVRLVLHFDIPGSLEAYYQEAGRAGRDGQPAACVLLF
                     HERDVATQEYFIAQAAKDSEGAERAERMTTLLQDFLGYVSASTCRQVAILDYFSDQAE
                     LALGLCGLCDRCVAHPSRMVSPETAASAKAIMNTVSWCGGRFGMGRIVDILRGSRSKA
                     LLAYGAEDCPTYGICRAQTKVSVTNLVKALIGSGYLQVQGTEYPTLDLTPKGREAVQG
                     LRTVAVEQTEDYPSNGSVKKPRRPHAIMAACQVSLPDRQLVERLRRLRAQLAEEEGVA
                     PFLIFHDRTLKAIAGYRPETSAALLEIPGIGEMKAERYGRRVLAVVNEYQ"
     gene            complement(4068..>4484)
                     /locus_tag="VN039_08890"
     CDS             complement(4068..>4484)
                     /locus_tag="VN039_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46118.1"
                     /translation="GRAVMGNWIPNDPTLVAQILKISAAYSPPPPEGFISPMTWGIES
                     HVVERFTGAGVPKERISFVRDTYTFNYPGTPAAFVDTFRRYYGPTMNAFDASEKNGRA
                     ADLQKELETLFGNQNQSANKQATSIPATYLRVTVVV"
BASE COUNT         1065 a         1339 c         1216 g          864 t
ORIGIN      
        1 tcgctcgctg cggccttgct ggacggccat tttgaacaac ctgccacgat gagacattaa
       61 tgccggcctg tccaactgtc tatagatact cataataccg cacaggtcaa tgggacgcta
      121 cttgtatgta ttgggtcgcc agtcctaatc atccaggctc ggacggacaa ggtatggccc
      181 atagtgaagt gcgaagagta catacgcacc accccaacag agtgccgata gaccaagtat
      241 tgtatgggtg agagtcgtgg gcgtctcgga atgcggggca aagattcgca ataacgcccc
      301 gacgttgacc agtagataga ttgccacggt gagccgatcc gcatgacgtg tgcgtcctgt
      361 gtgcccgaga cttgcgcggg tcatcaccgc caacgtcatc gcccccatcg ctccggtggt
      421 aagaagatga accgcattct cggtggaaaa tccaatccct agaattgcca cgccgagagc
      481 tatgagaaac aatcccaccc atccatagcc cacgtgcaga atcaagacga gcggctcacg
      541 ccacgtacgc catcctcccc aactcaacaa gcgcgccata ctcgccgctc cggcgataag
      601 cagcatggtt cctgcccacc cactttccgg ttggaaaacc cacgtgataa cgcccgcaag
      661 gaccaagatg atcgcgatgc cgtctattgg agaaaatatt tccggcagcc gtggcatgtt
      721 cttttcagcc aagaactcgc gagtaaaggt gggtgtgagg cgcccgccga tgatcgtcaa
      781 catcagcgtc atgaccgcca gggcaaagcg ttcggggaaa tcggtaggtg caccgcgcaa
      841 tgccgatacg tgaaagacaa tgttcgcaca ggcatagaaa ccgaccagca taccgatcgg
      901 cgccctgtcc caacagcctg ctgcgataat ctcccgccag acataggccg ccagcaagac
      961 caaaaaggct ccgtcgacca cagctgagaa cgcagaccca aaccacggag cgaccacaag
     1021 caatcgtcct gccagccaca gcagaaacag caccagcaac ggcatgcctc tcagcggcat
     1081 gcggtcggtc cagttaggca tggccgtcag cagaaatcct gcaatcaatg ccggtatgaa
     1141 cccaaacagc atctcatgga catgccactc gcgcggggaa taaagaaacg ccgcgtacac
     1201 accgcccgcg aacaggacaa cccagacgag gatggccaag ctgccaaaca gtgccgcgcc
     1261 cagaaagaac ggccggaatc catacgacaa gaacgccgga ccggtgtagg gaggaaacgt
     1321 gggttcgtca tcactgtcgt tctgagccgc agccgactgg ctgacgattg ggatttcttt
     1381 ggatggcata acggaatgat tctcggcggc gagttgagga cttgtcaacc aagagaatac
     1441 gttgcaaacg ggaaatgact tcagacagca agtaaagagg gaggataaca agaacgaggg
     1501 gtcgacactg agcatccacc agcggccaac cgaagttggg cattttctac gtaagtagcc
     1561 ctgtatcatt ttgtcttgtt gaacgtatca tttgagatac gatccccaca tggaatccat
     1621 actgaccgat gaagaaaccc atggaagggc gctcagatcc cgcattgatt gccgtcagtc
     1681 ctggcacgct atcgccgatt tcctggtggc accatcattg cttatgcact gaagcaacga
     1741 gtcgacggat cgcaaatagg cttggtgatg acgactgttt accgaggagg gcttcccatg
     1801 acgtgtacca gatgcagcgg gctcatggtc gcggatcatt tactggatat gcaagaaagt
     1861 tatctgccga tgtggatgcc ggcactccga tgtctggctt gcggcaatat cgtggactca
     1921 ttgattcact atcaccgcgc ggttcggcgc actcgcagag ccacgcggct cgcggcgcag
     1981 ttggcgaaac gttcgaccgt tcgaagcaac ccagccggtg gtggcgtcgt gggcttgagc
     2041 gctgtggttt cggctggacc ttctgtgaag ttgaagccgg caaccgacca agggctgccg
     2101 gtgatttgag aggtgacgta gaggtaggat tctttcacat cttgatgaaa gtcatggcgc
     2161 tattgatatt cattgaccac agccagcacc cgacggccat accgttcggc tttcatctcg
     2221 ccgatacccg gaatctccaa caaagctgcc gacgtctccg gtctgtaacc ggcaatggct
     2281 ttcaacgttc gatcgtgaaa aatgaggaat ggcgcgacgc cctcttcctc agcaagttga
     2341 gcgcgaagtc gcctgagtcg ttcgacaagt tgtcgatccg gcagtgaaac ctgacaagca
     2401 gccatgatgg catgcgggcg acgcggtttc ttcactgatc cattggacgg atagtcctcc
     2461 gtctgttcta cggctacagt gcgaagccct tgcacagcct ctcgtccttt gggcgtcaga
     2521 tcgagcgtgg gatactccgt accttggacc tggagatacc cggagccgat gagggccttc
     2581 acgagatttg tcaccgatac cttcgtctgg gcacgacaga tgccgtaagt cggacagtct
     2641 tctgcaccgt aggccagcag cgcttttgaa cggcttccgc gaagaatgtc gacgattcgc
     2701 cccataccga accgtcctcc gcaccaggac acagtattca tgattgcttt cgcagacgca
     2761 gcggtctcgg gagagaccat ccggctcgga tgcgcaacac agcgatcaca caggccgcaa
     2821 agtcccaggg ccagctcagc ttgatcgcta aagtagtcaa gaatcgcaac ctgtcggcaa
     2881 gtcgacgccg acacataccc caggaagtct tgcagcagtg tcgtcatgcg ttccgcacgt
     2941 tcggcgcctt cggaatcctt cgccgcctgt gcaatgaaat attcctgcgt ggccacatcg
     3001 cgttcatgaa acagcaacac acaggcagcg ggctgcccgt cacgacccgc gcgcccggcc
     3061 tcctggtaat acgcctccag actgcccgga atgtcgaaat ggaggaccag tcggacatcg
     3121 ggcttatcga tacccatgcc gaaggcattc gtcgcgacca ggattctcaa cgtccctcgt
     3181 cggaaatcgt catgcacgag tcgccgttgc tcatccgaga gaccggcatg gtagtagccg
     3241 accgcttggt gggattgtcc caaccagccc gcaacctctt ccaccgtccg gcgggtcgcg
     3301 caatagataa ggatcgtgcc cttctctgtc ccgcaaacca agcgctccac catccccagt
     3361 ttctctcgga ggctctgaca atgatggacg gacaaggcca gattcgcccg ccgaaaaccc
     3421 gtgactaatc ggaacggatc acgaagcgac aaccgctgac acagatctgt ctgcacacgc
     3481 gcggtggccg tggccgtcaa cgccaagcag ggtggattcg tgagttcccg acgcaggcga
     3541 ccgatcttga gatagtcggg cctgaaatca tgaccccact gggaaatgca gtgagcctcg
     3601 tcaaccacca gtagcgaaac ccatagggaa cgcaacagtc ggagaaaccc ttcatgctgc
     3661 atccgttccg gcgccagata gagcagctga actcgccgct gctggagatc ctgcatcacg
     3721 cggctctttt ccaaaccgct gagaccggag tgaaacgccg ctgcggcaat cttccgctgc
     3781 ctcatggccg tcacctgatc ttgcatcaac gcgatgagcg gagaaatcac cagcgtcagc
     3841 cctggcaaaa gggtcgccgg caattgatag cagagcgact tcccctgccc cgtcggcatg
     3901 accgccatcg catcgcgccc cgccaacacg gcgcgaatga cggcttcctg gcccggccgg
     3961 aacgtcgaaa acccgaaccg ttcgttgagt tgtcgagtca gattatccac tgggaagctg
     4021 aatcagaaag aaagcatgcc gaccacacgc gcttacacac caatcgatta tacgacaaca
     4081 gtcacacgca gataggtcgc tggaatagag gtggcctgct tatttgcgct ctggttctgg
     4141 ttcccgaaca gagtctcaag ctccttctgc agatcagccg cccgaccatt cttctctgag
     4201 gcatcgaacg cgttcatcgt gggtccatag tatcttctga aggtatcgac gaacgcagcc
     4261 ggcgtacccg gataattgaa cgtataggta tctcggacga atgagatccg ttctttggga
     4321 acccccgcac ccgtgaatcg ctcgaccaca tggctctcga taccccaggt catcgggctg
     4381 ataaagcctt ccggcggagg tggcgagtaa gcggcgctga tcttcaagat ctgcgcgacc
     4441 agcgtcgggt cattaggtat ccagttgccc atcaccgccc ggcc
//
LOCUS       DATMTI010000558         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_274974, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000558 DATMTI010000000
VERSION     DATMTI010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274974"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VN039_08895"
     CDS             complement(<1..444)
                     /locus_tag="VN039_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWV46119.1"
                     /translation="MSAWLESLGLDRYRELFEQNAITWDVLPELSDGDLASMGVLLGH
                     RKQLLRAMAQLSQRSEGDHASSLPLTTANGITPSSSSRDQAERRQLTVMFCDLVGSTA
                     LAQRLDPEDAQAAIRRFLDTCSEAIGRFNGYIAKYMGDGLLVYFGY"
     gene            complement(737..>2161)
                     /locus_tag="VN039_08900"
     CDS             complement(737..>2161)
                     /locus_tag="VN039_08900"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HWV46120.1"
                     /translation="TFSKLGQVLSTRPDLLPPEYIEELTMLQSHVPPMPESEVVRVSE
                     QELGVPWEDVFESIDPKPLAAGTIAQVHRATLDNGDRVVIKVQRPTARADIEQDLALL
                     EIFAQKVGQRPALNQVVNMEAVFKHLSTSLHRELDFHQEIGNIDRMRTVLADYDRLAV
                     PVVHHKLSTSRLLVMEEIQGVPIAQAPEGPERIETARQLLESYYKQIIVDGFFHADPH
                     PGNLMWWKERIYMLDFGMVGAVDDNVREHLLLLLMALWKEDVVFLGDVTLMMTGSLDR
                     SDLDVPRFQSEIGEVMAKYRKAALAEMQIGPLLQEMSAVGFRHGVPLPASLTLAAKAL
                     AQVQLATANLDPKLNPYDVAGKFLTRVMIKRMGASLDPKALVYQTQKLKVRAERVIEA
                     IEHLIGARPGQKLVVNFQADSLEEMVRRTGRRLTLGLTAAASILASGLTATSATVAGW
                     IPLTFGIVAGLLTVGLVLDLIRGR"
BASE COUNT          506 a          623 c          577 g          455 t
ORIGIN      
        1 gtatccaaag tacaccagaa gtccatctcc catgtatttg gcaatgtagc cgttgaatcg
       61 gccgatcgct tcactacagg tatcgagaaa acgtcggata gccgcttggg catcttcagg
      121 gtccaaccgt tgagccagtg ccgtcgaacc caccagatcg caaaacatga ccgtcaattg
      181 acgacgctct gcttgatctc gactggaaga ggacggcgtg attccattcg cggtggtcag
      241 gggtagagag gacgcatgat cgccttcact tctttgtgac agttgtgcca tggcgcgcaa
      301 gagttgcttt cgatggccca gcaaaacacc catcgacgcc aaatctccgt cactcagctc
      361 aggcaagaca tcccatgtga tggcgttctg ctcaaacagc tctcgatagc gatcaagacc
      421 aagactctcc agccatgctg acacgcgatc atccatgatc atctgaattg aagacgaatg
      481 gtcatgcacg gatcgatgac gcgcacgaga cggaccagtg ccctattgca gacagcgagt
      541 ccgtcattga taatgaccac atgatcaacc gcgcgatgaa gatgcttcat cagccccttg
      601 tacgaaaaat agagtaccgt ttcatctcca tcggaatgtc cgtcatcgca tacctactcg
      661 agagagtgat cctacgttcg attaagaaca gcgaagcgaa accctcatca cgacgcacgg
      721 cgaatggaag aagtccttaa cggccgcgta tcagatcaag gaccaatccg acagtgagta
      781 gcccggcgac gatcccgaac gtgagcggga tccaacctgc gacggtggcc gacgtagcgg
      841 taaggccgct cgcaagaata ctggccgcgg cagtcagacc cagcgtcaaa cgccgcccgg
      901 tacggcggac catctcctca agcgaatcag cctggaaatt gacgaccagc ttttggcctg
      961 gacgagcccc gatcagatgt tcgatggctt cgatgactcg ctcggctcgc accttcaact
     1021 tctgcgtttg atacacgagt gcttttggat cgagcgaagc ccccatgcgc ttgatcataa
     1081 cgcgcgtcaa aaacttccct gccacgtcat acggatttag ttttgggtcg agattggccg
     1141 tcgccagttg tacttgggca agtgccttgg cagcgagcgt caacgacgcc ggcagcggga
     1201 cgccgtggcg gaatcctacc gcgctcattt cttgaagcag cggcccgatc tgcatctcag
     1261 ccagagccgc cttgcggtat ttcgccatga cttcgccgat ctcactttga aaccggggca
     1321 catccagatc gctgcgatca agagaacccg tcatcatcaa tgtgacatcg ccgagaaaga
     1381 ccacgtcttc tttccacagt gccatcagca acaacaagag atgctcgcgc acattgtcat
     1441 cgacggcacc gaccattccg aaatcgagca tatagatgcg ctctttccac cacatgagat
     1501 tgcccgggtg gggatcggca tggaagaagc cgtcgacgat gatctgtttg tagtaactct
     1561 ccaagagctg gcgggccgtt tcgatgcgct ccggaccttc cggcgcctgc gcgatcggaa
     1621 caccttgaat ttcttccatc accaaaagtc gagaggtcga cagtttgtgg tgcacgaccg
     1681 gcacggccag acgatcatag tctgcgagaa ccgtccgcat ccggtcgatg ttgccgatct
     1741 cttgatggaa gtcgagttcc cgatggagcg acgtggagag gtgcttgaaa acagcctcca
     1801 tattcacgac ttgattgaga gcaggtcgtt gcccgacctt ttgagcaaat atttcgagca
     1861 aggcaagatc ctgttcgatg tcggctcggg ccgtgggccg ttgcactttg atgaccactc
     1921 gatcaccgtt gtcgagtgtg gcccggtgga cttgcgcgat ggtcccagct gcaagcggct
     1981 tggggtcaat cgactcaaac acatcttccc acggcacccc caactcttgc tcggagaccc
     2041 ggacgacctc actctcgggc ataggaggga catgactctg caacatggtc agttcttcga
     2101 tgtattccgg agggagaagg tccgggcgcg tcgacaatac ttgtccaagc ttcgagaagg
     2161 t
//
LOCUS       DATMTI010000559         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_234343, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000559 DATMTI010000000
VERSION     DATMTI010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234343"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1246)
                     /locus_tag="VN039_08905"
     CDS             complement(join(12..958,958..1246))
                     /locus_tag="VN039_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HWV46121.1"
                     /translation="MGNATRYSPEVRERAVRLVLEQVKDYSSQWAAIRSIAEKFGCSP
                     ETLREWVRKEERDHGDRAGLSTNEREELKRLQREVRELRRTNEILKAASMFFGEGGVR
                     PPTEMMVSFVDQNRTAWGVEPICDALPIAPSTYYEVKAGEADPSKLSQRRRRDSELVV
                     LIQKVWDENFQVYGPRKVWKQLVRERVAVGRGRIERLMRQMGLQGTRRGDKTKRTTFW
                     DPSIPTPEDLVQRQFHAIAPNQLWVADFTYVATWSGFVYVAFVIDAYAKTIVGWRVST
                     RMTAELTLDALEQALWARPVNAGLIHHSDHGSQYLSIRYSDRLAQAGVDASAGTVGDA
                     YDNALAETINGLFKTEVIWKRGPWKGREDVEFATLKWVYWYNNRRLLSSVGYVPPAEF
                     EAAYYTRESSRLVEQGLRQ"
     misc_feature    complement(852..968)
                     /locus_tag="VN039_08905"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1297..>1507
                     /locus_tag="VN039_08910"
     CDS             1297..>1507
                     /locus_tag="VN039_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46122.1"
                     /translation="MAGYDSLADYWKLTKDGKLQYDGQGYLRDANGLYINTDGSRSQN
                     PIAGKTVGADGVETGLLNILNGGGTS"
BASE COUNT          307 a          453 c          406 g          341 t
ORIGIN      
        1 gagcggagac attattgtct gagtccttgc tcgacgagcc gactcgattc cctagtatag
       61 tatgccgcct cgaactcagc gggcggaaca taaccaacag acgatagcag ccgccggttg
      121 ttgtaccagt agacccattt gagggttgcg aactcgacat cttcgcgacc cttccagggg
      181 ccacgtttcc agatcacttc ggtcttgaat agcccgttga tggtctcagc caaggcgttg
      241 tcgtaggcgt cgcccacggt accggcggag gcatcgactc cggcttgggc cagccgatcg
      301 ctgtagcgga tcgacaggta ctgacttcca tggtcgctgt gatggatcag ccccgcgttg
      361 accggacgag cccatagagc ctgctccagg gcgtccaggg tcaactcggc tgtcatcctg
      421 gtcgagacgc gccacccgac aatggtcttg gcataggcgt cgatgacgaa cgcgacgtac
      481 acgaagcctg accaggttgc cacataggtg aagtcggcca cccagagctg gttgggcgct
      541 atcgcgtgga actgacgctg caccagatcc tccggtgtcg ggatgctagg gtcccagaag
      601 gtggttcgct tggttttgtc accccgcctc gtgccttgaa gccccatttg gcgcatcagc
      661 cgctcgatcc ggcccctgcc aacggccacg cgctcacgca ccagttgctt ccagaccttc
      721 ctcggaccat aaacctggaa gttctcgtcc caaacctttt gaatcagcac taccagctcg
      781 ctgtcacgac gacgtcgctg tgatagcttg ctcggatccg cttcaccagc tttgacttcg
      841 tagtacgtcg atggagcaat cggcaaggca tcgcagatcg gttcgactcc ccatgcagtc
      901 cggttctggt cgacaaaact gaccatcatt tctgttggcg gtcgaactcc gccttcgcaa
      961 aaaacattga cgccgccttc agaatctcgt tggtacgtct gagctcccga acctcccttt
     1021 gaagccgctt cagctcttca cgctcgttgg tgctcagtcc cgcgcggtcg ccgtggtccc
     1081 gctcttcctt tcgaacccat tcccgcagcg tctccggtga acatccaaac ttctcggcga
     1141 ttgatcgtat cgccgcccat tgtgacgaat agtctttgac ttgttctaaa accaaccgga
     1201 cagctcgttc ccgcacttcc ggcgaatacc tcgttgcgtt ccccatgacc tcatcctctc
     1261 agaagatttg gtctccggca aaccccgggc gattcagtgg cggggtatga ttcgttggct
     1321 gactattgga aactaaccaa ggatgggaag cttcagtacg atggtcaagg gtatttgaga
     1381 gacgccaatg ggttgtacat caatacggat ggaagccgat cacagaatcc gatagctggt
     1441 aaaacagttg gagccgacgg agttgaaact gggttgctaa acatcttgaa cggtggtgga
     1501 accagct
//
LOCUS       DATMTI010000560         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_742077, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000560 DATMTI010000000
VERSION     DATMTI010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742077"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VN039_08915"
     CDS             <1..1133
                     /locus_tag="VN039_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46123.1"
                     /translation="LRPYWVLLKAPEIIDWDIDPFERLLYLKRKEITSGRNQRNARRV
                     YEKYTEWFPDRVDITYVDVTNAHEPKIVPSMGTTVPNRVGEIPIQIVRYERSKRYPYM
                     GNSFLRDFSNNNLQVMNLTSLIDEFNYKQCFNILAKEEDANALPLRDGNDGVVGVGNV
                     MEYPKGAKPPAYITPPADPAKFMQSERDYIIQQMFKRAAQDMMSELFNGEGASGFSQA
                     QSFAKTVPFISNRADTLEAAENALMTRTLKYLNKTWDGKIKYKDRYELTNITDALTQL
                     LMLFRDLQLPSETFVKEELKRLVHEMDGKLPTDTLNKVLHEIDELDFDEWQEVQKEAL
                     VGKPSSPGEQNQPKSTGTTAEAASEAKAQTASTSKIRNKNNK"
     gene            1661..>2229
                     /locus_tag="VN039_08920"
     CDS             1661..>2229
                     /locus_tag="VN039_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46124.1"
                     /translation="MPTEAEIQEQNTGTSAGKTDGKVVFDEAQQAKINEIIESRLGRE
                     SSKYKAELDSRNAKITQLESDLAAARDAAKHGKGSSKDDANDEVSRLRAEIEEVRRVN
                     STRESDYKSLQAQIAAEKKAAEDARSEALNTRKNAAIMSAADKVGFIDLDDVVSLTER
                     HVVWNDDHKQFVVLNPAGQPRLNASFEPM"
BASE COUNT          634 a          549 c          586 g          460 t
ORIGIN      
        1 agttacggcc ctactgggta ctacttaagg ctcctgagat tatcgactgg gacattgacc
       61 ctttcgagag attgctttac ctgaagcgca aggagattac ctccggtaga aaccagagaa
      121 atgcgcggcg ggtttacgaa aagtataccg agtggttccc agatcgcgtt gacatcacat
      181 acgttgatgt cacaaacgcc catgagccga aaatcgttcc gtcgatgggg actacggttc
      241 ctaatcgggt aggagagatc cccatacaga ttgtgcggta tgagcgttct aaacgctatc
      301 cgtacatggg caatagcttc cttcgtgact tctccaataa taatcttcag gtaatgaacc
      361 tgacgagcct tattgacgag ttcaactaca agcagtgttt caatattctg gctaaggaag
      421 aggatgctaa cgctctaccg cttcgggatg ggaatgacgg agtagttggc gtaggtaacg
      481 tgatggagta cccgaagggc gcaaaacccc cggcatacat cacaccgccc gccgatcccg
      541 ccaaattcat gcagagcgaa cgcgactaca tcattcagca gatgttcaag cgtgcggctc
      601 aggacatgat gagcgaactt tttaatggcg agggtgctag cggattcagt caagcccagt
      661 cgttcgctaa aacggtcccg ttcattagca accgcgccga taccctggaa gcggctgaaa
      721 atgcgctgat gacgcgcaca ctcaagtact taaataagac ttgggacggc aagatcaagt
      781 acaaggaccg ctacgagctt accaatatta cagacgcttt gacacagctt ttgatgctgt
      841 tcagggatct ccagttgccc agtgaaacct ttgtgaaaga ggaactgaag cgactggtgc
      901 atgaaatgga cggtaagcta cctaccgata cgctcaacaa ggtcttgcat gagatagatg
      961 agttggattt cgatgaatgg caggaagtac agaaggaagc actggtaggg aaaccctcca
     1021 gtcctggtga gcagaatcaa cccaagtcaa ccgggactac cgcagaggca gcgtccgaag
     1081 ctaaggccca gaccgcttct accagcaaga tccgcaacaa gaataacaag tgaggataat
     1141 taattgattt caagaggaac agtcgccgca ggccgcagaa gtcccacgaa tgtaatcggg
     1201 gatttcagcg cggaatctgc aaaggctgcg ggtgctcggt ctaaccagga aaagagtcag
     1261 actcaatccg ctaaattccg caatgtgccc gtagctaagg gcaaatataa cgaaggtgaa
     1321 gtcgttcgta tgcaggccaa tacaaaaggc acgggcgtag tcggcagacc ttcggttctg
     1381 ggtcaggcgg gacgtgattt cacgaagcct gctgtgcaga acccagatgg cgcatcgagc
     1441 cgccctggtt cacgccgcaa gtaactcagt tttgaattaa caccagggtc tacgcttctc
     1501 gggtattact ccgcttcacg cggtattctc gtaagtgggt agcttaaacg tgggctaaaa
     1561 cgtatccccg cctggtgtct aacaacttag gcccttacgg tgacctcgtc cggaagggcc
     1621 tttacatttc tacgagggct taaaaagaca aaacgaaaac atgcctactg aagcagaaat
     1681 tcaggaacaa aacacgggta cgtctgccgg taaaacagat ggaaaagtcg tattcgacga
     1741 agcacaacag gccaaaatca acgagatcat cgagtctcgt cttggacggg aatcttccaa
     1801 atataaagct gaactagaca gccgtaacgc gaagatcacc caactggaat cagaccttgc
     1861 cgcagcacgt gatgctgcca agcacggcaa aggttccagc aaagatgacg ccaacgatga
     1921 ggtatcgcgg cttcgcgctg agattgaaga ggtcaggcgc gtgaacagta cccgcgaatc
     1981 agattataag tccttgcaag cgcagatcgc ggcggaaaag aaagccgccg aagatgcccg
     2041 ctcggaagca ctcaacaccc gtaaaaacgc tgcaatcatg agtgcggcgg ataaggtcgg
     2101 ttttatcgac cttgatgatg ttgtttcgct aaccgaaaga cacgttgtat ggaacgatga
     2161 ccacaagcaa tttgtggttc tgaatcccgc tggtcaaccg agactcaacg cctcattcga
     2221 gccgatgag
//
LOCUS       DATMTI010000561         1776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_360878, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000561 DATMTI010000000
VERSION     DATMTI010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360878"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(481..1083)
                     /locus_tag="VN039_08925"
     CDS             complement(481..1083)
                     /locus_tag="VN039_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46125.1"
                     /translation="MHQRISTPIGPKTKKPLDIVVKFGLAVFAGSFALIWGGMYLSRP
                     DRSIPPYTVGAQSGHIVTTDVPRGTTDEEIESLVKRFRKVGHQTHDFARMKIYPTTPG
                     DPGGPYGQIMIYVFDDHGWTDPEVLAKYMAGDAVVIKDYERSMRGYYRLQDQDEEGGI
                     GPLTKNGRTTSDTRILFKGLVTDPLPVETEAEQGISISPL"
     gene            complement(1109..>1776)
                     /locus_tag="VN039_08930"
     CDS             complement(1109..>1776)
                     /locus_tag="VN039_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HWV46126.1"
                     /translation="EFREDRYIVMGTKKGIIKKTELSAFSNPRQGGIIALGLEGGDRL
                     IGVQLTDGQREILLGTRQGITIRFKEEEVRPMGRTAYGVKGITLEEGNEVIGMETITP
                     DSTTSVLTVTEGGYGKRTPVGEYRVQGRGGKGIISVKTTERNGLAVGFLQVRDGDEIM
                     LIAAQGKVLRCKVDDIREIGRNTQGVRILDLDGEGDRVVGVARLAEAVEREDVGSDEI
                     PEA"
BASE COUNT          332 a          541 c          470 g          433 t
ORIGIN      
        1 cccgacaccg acgccttgaa ctatttctcc gctattgcct atgatgccgc atgacgggcc
       61 ctggtacgct tgattccttc ggcagcatcc tttccggtct ttccaagcgg cttggtctcg
      121 aatcgagact ggtggaactt cgcttacagc accggtggcg cgacatcgtg ggtgagccca
      181 tggcttccca cacttggccg gcccacattc gcttcaaaaa gctctacctg atcgtcagaa
      241 attcggtctg gctgcaacag ttgacgttcc tcaaaccggc gctcttgacg aagttgcagg
      301 cagagtcggg accggggtct gtaacagaca ttgcgtttcg tgtcggagag tttcccggtg
      361 aaacggaggc ttctcccgca agcctcacca ccgacctcgg ggcgcctcag tcggagaaat
      421 cctcggctga gctgatatcg cataccgcca tgatccaaga ctcgtccatc cgtgagcggc
      481 ttagagaggt gatatcgaga taccctgttc agcttccgtc tcgaccggca agggatccgt
      541 cacaaggccc ttgaagagga tccgtgtgtc gctggtggtc cgcccattct tggtgagagg
      601 gcctattcct ccttcttcat cctgatcctg caatcgatag tatccgcgca tggatcgttc
      661 gtagtccttg atgaccactg catctcctgc catgtacttc gccagtactt ccggatcggt
      721 ccatccgtga tcgtcgaaca cgtaaatcat gatctgtccg tacggaccac cgggatcgcc
      781 cggagtggtg ggatagatct tcatgcgagc gaaatcgtgc gtttggtggc cgacctttcg
      841 aaaacgcttc accagcgatt caatctcctc atcggttgtt ccgcgaggaa cgtccgttgt
      901 gacgatatgt cctgattgcg caccgacggt ataaggagga atcgatcgat cgggacggct
      961 gagatacatg cctccccaga tcagggcgaa ggatcccgca aagacggcga gcccgaattt
     1021 gacgacgata tcgagcggct tcttggtctt gggcccaatg ggagtcgata tccgttggtg
     1081 cattgaacag ccgcgcggct cggtcgggct aggcttcggg gatctcgtcc gaacccacgt
     1141 cctctcgttc aaccgcttct gcgagtcttg cgacgcctac gactcggtcc ccttcgccat
     1201 cgaggtcgag aatgcgaacc ccttgggtat tccgaccgat ttcacggatg tcgtccacct
     1261 tgcaacgaag cactttcccc tgcgcggcga tcaacatgat ttcgtcgccg tctcgtactt
     1321 gcagaaatcc gacagccaag ccgtttcgct ctgttgtctt tacgctgatg atgcccttgc
     1381 cgccgcggcc ttgcacacga tattcgccca cgggggtccg tttgccgtag cccccttccg
     1441 tgacggtcaa aaccgaggtc gtggaatcgg gggtgatggt ttccatgccg atgacttcat
     1501 tgccttcttc gagcgtgatc cctttgacac catacgcggt ccggcccatc ggcctcactt
     1561 cttcttcttt aaaccgaatg gtaataccct gtcgggtccc cagaagaatt tcccgctgtc
     1621 catcggtcag ttgcacgccg atcagtctat cgccgccctc aagtcccaac gcgatgatac
     1681 caccttgccg tgggttgctg aaggcggaca gttccgtctt tttgatgatg cccttcttgg
     1741 tacccatcac gatatagcgg tcctctcgga actctt
//
LOCUS       DATMTI010000562         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_827847, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000562 DATMTI010000000
VERSION     DATMTI010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827847"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VN039_08935"
     CDS             <1..624
                     /locus_tag="VN039_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46127.1"
                     /translation="VYVFFAGRSLVEGGTGAISLVPYDGMPSGAKQLYSLVRLQEVLY
                     RLPIRRAILMFDVSLDPSPGAGLADIPSPAWESSVSEARKDVEMWMVSNRGLQEAHAY
                     DEGKHGLFTYHLLRGLQGAADVDRDGTVTAGELCTYARGEAARVAREQFGNKQDALCV
                     PGIGREGMVRIYPVARGNNPKPVPMQKQPNLPADSSGPAPNPMQVGP"
     gene            723..798
                     /locus_tag="VN039_08940"
     tRNA            723..798
                     /locus_tag="VN039_08940"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:756..758,aa:Thr,seq:cgt)
     gene            1157..1342
                     /locus_tag="VN039_08945"
     CDS             1157..1342
                     /locus_tag="VN039_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46128.1"
                     /translation="MTQLAQKLDKKLASWRPEVAAQVEQIVTDVIELADTDTLDLQPS
                     RTVVQEVFDTLDERQAR"
BASE COUNT          338 a          466 c          464 g          331 t
ORIGIN      
        1 gtctatgtgt tttttgccgg acgatcgctg gtagagggcg ggaccggcgc gatttcgctc
       61 gtgccgtatg atgggatgcc ctccggggcg aagcagctct attccctcgt ccggctacag
      121 gaagtactct atcgacttcc gatccgacgg gctattctga tgttcgacgt ctcgctggat
      181 ccttctccag gcgccggtct tgccgacatc ccctcgcctg cctgggaatc ttccgtgagc
      241 gaagccagga aagatgtgga aatgtggatg gtgagcaatc gaggcctgca ggaagcccat
      301 gcgtatgacg aaggcaagca cggcttgttt acgtaccatc ttctcagagg gttgcaaggc
      361 gcggccgatg tggatcgcga cgggacggtc accgccggcg aactctgcac ctatgctcgg
      421 ggtgaggcgg cgcgtgtggc gcgtgaacaa tttgggaaca agcaagatgc gctttgtgtt
      481 ccaggaatcg gacgggaagg catggtgcga atatatccgg tggcgagagg caataacccg
      541 aagccggttc cgatgcaaaa acaaccaaac cttcccgccg actcatcagg accggctccc
      601 aatccgatgc aggttgggcc ctagttaagt gaggcggcct gcttcattga cagtactcag
      661 cgatgaccgt tatcatgcca ggcacgacct cgcattccgt ctccctcctg tctcacctag
      721 gtgccggcgt agctcagttg gtagagcagc ggtttcgtaa accgcaggtc gctggttcga
      781 ttccagtcgc cggctccaga aaagaaaaat cgaaccagcg acctgagggg ttcgaagggg
      841 atatgtcccc ttcgtgggga ttatacaagg gggctggccc cctttgtagg aatcggtgag
      901 gcaagtctca gagccgaacc gatgacaggt tgctccgttc aatccgggtc gccggctcca
      961 aaccacactt cgtgtggccc gtgcgctatt catcgtagta tcgagcatga cgcacgcgga
     1021 taaacaccta cagtcctcgc cgaattcagg acgcacgact gggagcggag ggtctgacag
     1081 tcatcagtag cagagcatcc ccgatttcag aaagccaaac cttgcgaggt gaagagaaac
     1141 atgctaccgt ccgcttatga cacagctcgc ccagaaactc gataaaaagc ttgcctcctg
     1201 gcggcccgag gtggcggctc aagtggagca aatcgttacc gacgtgatcg agttggccga
     1261 caccgacacc ctggacctgc aaccttcccg aaccgtcgta caagaagtgt tcgatacgct
     1321 ggatgaacgc caagccaggt gatatctggc tggccgacct cggtttagct gccaagaccc
     1381 gccccgtgct catcgtctct cgtcatgatc cggatgcgcc ccgcgcactc atcacgtacg
     1441 ttccggggta gtcgctataa gatccctctc gggagccttc cctttttgca cgaagcgtct
     1501 ggccaacgtt caaggtatcg ggtctctgat cgaacccaga cttgaacgca aactaggccg
     1561 gctttccact gctgcctgga gagcctccgc ttaggcccc
//
LOCUS       DATMTI010000563         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_827853, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000563 DATMTI010000000
VERSION     DATMTI010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827853"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="VN039_08950"
     CDS             complement(<1..1809)
                     /locus_tag="VN039_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWV46129.1"
                     /translation="MTASSTLILPARTHAVARDLRALLGAEKVKDDVPTITAYAVDAS
                     IYRVPPQAVVLVESEEDIAATVAYAVSRGIPLTPRAAGTNLTGSAIGSGIILDVSRLN
                     RILEVNREERWARVQPGIILAELNKRLSSQGLLFGPDPSSGDMCKLGGMVANNSSGPH
                     TLRYGSVKDNVQSLRLCLTSSTWIEARSYDLDDPSLERLLTTAPALRDVLMMTQAHTT
                     LIAAKRPTVSKNSCGYNLFGLADGLARGSFDLPKLFVGSEGTLGVVSEARLCLVDKPK
                     ATLTALIHFRSLEEIGEAVPQLLTLQPSALEVMDANTLNLIGRGKHGIPADAAATLLV
                     ELDADSLEIDLCERAEAMATVCRPYELAAELTLAFDAERREQLWKARKALYPTLYRFD
                     PKKKPINFVDDVVVPAGRIGELIRYLEDFFEGQRVPVAIFGHIGNGNAHIVPLLDVND
                     SGDFEKMVSAYQEIHATVLNRFGGSICGEHGDGRVRAAFVKTMFGEELYSLFVRVKKA
                     FDPGNVLNPGIKLSAASFTEHIDYTRLSKSCATCAKCNSVCPVYDVFQSEDMSSRGWF
                     EIVTAKDYSYLNSKRVVEACLNCKSCRTICPAGVDVS"
     gene            1898..2854
                     /locus_tag="VN039_08955"
     CDS             1898..2854
                     /locus_tag="VN039_08955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWV46130.1"
                     /translation="MERPTLYITRKLPQSVLDAIPGQYRMPTEPTDQPPTEDELRRGF
                     AEAQAVICTLTDRIDASLLSHVTKLKVIANYAVGYNNIDLPAATQRGIIVTNTPDVLT
                     DATADLTWALLLALTRRVVEGDVWVRTGHWPGWTPTQMLGADVSGKTLGIIGMGRIGQ
                     AVAKRAAGFRMSVIYAGRQSIAGPPGSSWTWRPLDEVLVESDFLSLHVPLTEATHHLI
                     DRRKLALMKPTAFLINTSRGPVIDEDALVSALEGKTIAGAGLDVYEQEPVVPVRLMSL
                     PNVVLLPHLGSATLETRVRMGRICLENIAAVLGGRPAPNRVN"
     gene            complement(2882..>3862)
                     /locus_tag="VN039_08960"
     CDS             complement(2882..>3862)
                     /locus_tag="VN039_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV46131.1"
                     /translation="LAEAVRRIDERQFAGAEAILNQILTREPGNPQVCQLLARLHLQR
                     GDVQVALGEYRFLAGAALRAHDLALAESLIQEFLSADPNSVPLLELNGELHEEKGNHG
                     EAALQYAKAIELLLEHPEPGMESLHEELFEKVRSLSSDEVLLNRLAARIKRETSEEQS
                     EFARIAKDNGSRQTRVESDAVDPAVAPPANIEESAAPPDYEAHYALGVAYKNMGLYEE
                     AKEEFQVSMNSDSFYLDSALMTAVCLKEGRYIAQAIQGLEPVLADSRCRGAKAQAIRY
                     ELGLLYEAEEQWEKAVHAFESIPSFHDVPQRLAAIKGRQVEGEVGFRYAV"
BASE COUNT          807 a         1148 c         1070 g          837 t
ORIGIN      
        1 agatacgtcc actcccgctg gacagatggt acggcaggat ttgcagttca gacatgcttc
       61 caccacccgt ttggaattga gatagctgta atccttggcc gtcacgatct cgaaccagcc
      121 gcgtgaactc atgtcctccg attgaaagac atcgtatacg ggacagacag aattgcactt
      181 ggcacaggtc gcgcaggatt tggacaatct ggtgtaatcg atatgttcgg taaatgaggc
      241 cgcgctcagc ttgatgccgg gattgagcac attcccgggg tcgaacgcct tcttgacccg
      301 cacaaagaga ctgtagagtt cttcaccgaa catggtcttg acgaacgcag cacggacacg
      361 gccgtcgcca tgttcaccgc aaatcgagcc gccgaatcga ttcagcactg tggcatggat
      421 ttcctgatag gcgctcacca tcttctcgaa gtcgccggaa tcgtttacgt ccaacagggg
      481 gacgatgtgc gcatttccat ttccgatatg gccgaagatc gccaccggca cgcgctgccc
      541 ttcgaagaag tcctccaggt atcggatcag ctcgccgatg cgtcctgccg gcacgacgac
      601 gtcatcgaca aaattgatcg gcttcttctt cggatcgaat cgatagagcg tcggatagag
      661 cgcttttcgc gctttccaga gttgctcccg acgttcggca tcgaacgcca gcgtcagctc
      721 cgccgccaac tcgtaaggcc gacagacggt ggccatggct tcagcccgct cacagagatc
      781 gatctcgagg gagtcggcat ccaattcgac cagcaaggtc gccgccgcat ccgccggaat
      841 gccatgtttc cctcgaccga ttaagttgag ggtgttggca tccatcacct ccaaggcgct
      901 tggttgaagc gtcaagagct ggggcacggc ctcaccgatc tcctcgagac tccggaagtg
      961 aatgagtgcg gtcaacgtag ccttaggctt gtcgaccagg cagagcctgg cttcactcac
     1021 tacacccaac gtcccttcgc tgccgacaaa taacttaggg aggtcgaaag agccccgtgc
     1081 gagcccgtcg gccaacccaa agagattgta tccgcagctg ttcttgctga cagtcggtcg
     1141 ttttgcagcg atgagggtcg tatgggcttg tgtcatcatc aggacgtcac gcagggccgg
     1201 cgccgtcgtc aggagacgtt ccagcgatgg atcatcgagg tcataggatc gcgcttcgat
     1261 ccatgttgac gaggtgaggc agagccggag gctctgaaca ttgtctttta ccgatccgta
     1321 acgaagagtg tgcggtcccg aagaattatt ggcgaccatg ccgcccagct tgcacatgtc
     1381 cccgctcgac gggtccggcc caaacaacag cccctgcgag ctgagccgct tgttcaactc
     1441 cgccaaaatg atccctggtt ggaccctggc ccatctctct tcccgattca cttcgaggat
     1501 ccgattcagg cgtgatacgt cgaggatgat cccggagccg atggcggaac cggtcagatt
     1561 cgtcccggcg gcccgaggcg tgagtggaat acctcgtgat acagcatagg cgacagttgc
     1621 ggcgatgtcc tcttctgatt caacaaggac gaccgcctgt ggaggaacgc gatagatgct
     1681 ggcatcgacg gcataggctg tgatcgtcgg gacatcgtcc ttgactttct ccgctccaag
     1741 cagagcgcga agatcgcggg cgactgcgtg agttcttgcg ggcaagatca atgtggatga
     1801 agccgtcata gacaatcatg catcttagct cgcggactct tcgaagaacc agagtcgttg
     1861 tcggtcgcct acaaattctc ctacaatgga actgcccatg gagcgtccaa ctttatatat
     1921 tacccgtaag ttgcctcaat cggtgctaga cgcaatccct ggacagtatc ggatgccgac
     1981 cgaaccgaca gatcagccac caacggagga cgagctgcgt cgcggcttcg ccgaggcgca
     2041 ggcggtcatc tgtaccctga cggaccgtat cgatgcctcg ctgttgtccc acgtcacgaa
     2101 actgaaagtc atcgccaact atgcggtagg ctacaacaat atcgaccttc ctgccgcgac
     2161 tcagcgcggc atcatcgtga ccaacacacc ggacgtgttg accgatgcca ccgccgatct
     2221 gacttgggct ctgttgctcg cgctgacgag gagggtcgtt gaaggagatg tctgggttcg
     2281 aacaggtcac tggccgggat ggacaccgac acagatgctg ggcgccgacg tgtcagggaa
     2341 gaccctcggc atcatcggca tggggaggat cggacaggcg gtcgcgaaac gggcggccgg
     2401 gtttcggatg tcggtgatct atgccggacg tcagagtatt gccggtccgc ccggcagttc
     2461 gtggacctgg cgtcccctcg atgaagtact cgtggagtcg gattttcttt cgcttcatgt
     2521 cccactcacc gaggccactc atcatctcat cgatcgccgc aagctcgccc tgatgaagcc
     2581 gaccgccttt ctcatcaaca cgtcgcgcgg cccggttatc gacgaagatg ccttggtgtc
     2641 ggcacttgag ggaaaaacca tcgcaggcgc cggactggac gtctacgaac aagaaccggt
     2701 tgttccagtc cggttgatgt cgctgccgaa cgtggtcctt ctccctcacc tgggatcagc
     2761 cacgcttgaa acccgtgtcc gtatgggacg catctgtctc gagaatattg ccgcagtgtt
     2821 gggaggcaga ccggcaccga atcgggtgaa ctaagatatg agtgaaaaat gcggctgggc
     2881 cctacactgc ataccgaaaa ccgacttccc cttccacttg ccgccccttg atggctgcca
     2941 gtcgctgcgg gacgtcatga aatgaaggaa tagactcaaa tgcgtgaacg gccttctccc
     3001 actgttcctc cgcttcatag agtaagccca actcgtagcg aatagcttgc gccttcgcgc
     3061 ctcggcagcg tgaatcggcc aagacaggtt ccagcccctg gatcgcctgg gcgatgtacc
     3121 gcccttcttt gagacagact gcagtcatca gcgccgaatc gagataaaac gagtcgctgt
     3181 tcatcgacac ctgaaactct tcttttgcct cttcgtacaa tcccatgttc ttatatgcca
     3241 cccctaaggc gtagtgcgcc tcgtagtcgg gcggcgcagc tgattcctct atattcgccg
     3301 gcggagcaac ggcggggtca accgcatccg actcgactct ggtttgccgc gatccattgt
     3361 ccttggcgat ccgagcaaac tcgctctgtt cttctgaggt ctctctcttt attcttgccg
     3421 ccagtcgatt cagcaagact tcatcactgg acagcgatcg caccttttcg aagagctctt
     3481 catgcaggct ttccatgccg ggctcgggat gctccaacag gagttcgata gccttcgcat
     3541 actgcagtgc ggcttccccg tgattgcctt tttcttcgtg cagttccccg ttcagttcca
     3601 gcaagggaac ggagttcggg tcagcggata agaactcctg aatcagcgat tcggcaaggg
     3661 ccaggtcgtg cgcccgcaac gctgctccgg ccaagaagcg atactctccc aacgccactt
     3721 gcacatcccc tcgttgcaaa tgcaaccgag ccaagagttg gcagacttga gggttccctg
     3781 gttcccgtgt gagtatttgg ttcaggatcg cttccgcgcc ggcaaactgc ctttcgtcaa
     3841 tgcgacgaac ggcttcagcc ag
//
LOCUS       DATMTI010000564         1535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_445027, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000564 DATMTI010000000
VERSION     DATMTI010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445027"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="hemB"
                     /locus_tag="VN039_08965"
     CDS             <1..616
                     /gene="hemB"
                     /locus_tag="VN039_08965"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWV46132.1"
                     /translation="TTHGQDGLIDDTGYVMNEPTVEALIKMSLAQAEAGMDFVAPSDM
                     MDGRIGSIREALEDAGHIHTRILAYSAKYASAFYGPFRDAVGSSANLGKGNKHTYQMD
                     VGNSDEALREVELDLLEGADAVMVKPGMPYLDVLRRVKDSFGAPTFVYQVSGEYAMLK
                     AASQNGWLDEKSVVLESLTAFKRAGADAILTYYAIDAARWLRGE"
     gene            706..1065
                     /locus_tag="VN039_08970"
     CDS             706..1065
                     /locus_tag="VN039_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46133.1"
                     /translation="MLGWGVMVLRPEVASTGKPECLLARWTTGVSGLGWLEDLVKEGK
                     AIDLGGDGYPNRYPITLDLVLPILHRGLPSNDSPVVIGEDYLVPPGCNGKIEVNTAEL
                     VDCSNSESLVVEAWDQS"
     gene            complement(1131..1520)
                     /locus_tag="VN039_08975"
     CDS             complement(1131..1520)
                     /locus_tag="VN039_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46134.1"
                     /translation="MFSIISSLLIPMDRFATNTVKNLLNLSYVPPESLPAELVSYMVD
                     CTETARFRVKGRFAMRVDAIKTADVIVGNFILWLDSDGGRKSIGFHSVDSYLQETFEK
                     MVSPQRRAELFAHRVGVASDLVASARK"
BASE COUNT          328 a          408 c          455 g          344 t
ORIGIN      
        1 taccacccac ggccaggacg gcctgatcga cgacaccggc tacgtgatga acgagcccac
       61 cgtcgaggcg ctgatcaaga tgtcgctggc ccaggccgag gccggcatgg atttcgtggc
      121 gccgtcggac atgatggacg gccgcatcgg ttccatccgc gaggcgctgg aagacgccgg
      181 ccacatccac acccgcatcc tcgcctactc ggcgaaatac gcctccgcct tctacggccc
      241 gttccgcgat gcggtcggtt cgtcggccaa cctcggcaag ggcaacaagc acacctacca
      301 gatggacgtg ggcaacagcg acgaggcgct gcgcgaagtc gagctggacc tgctcgaagg
      361 cgctgacgcg gtgatggtga aacccggcat gccgtatctc gacgtgctgc gccgggtcaa
      421 ggacagcttc ggcgcgccga ccttcgtgta ccaagtgtcg ggcgagtacg cgatgctgaa
      481 ggccgcctcg cagaatggtt ggctggacga gaagtcggtg gtgctggagt cgctgaccgc
      541 gttcaagcgc gcgggggcgg atgcgattct tacctattac gcgattgatg ctgcgcggtg
      601 gttgcgcggt gaatagggca tccagttcag ccggcaatgg gttcttcttt gtttggaatt
      661 cggatccgtt gtctgtaggt ggatggatct gacggaggta actcaatgct cggttggggt
      721 gtcatggtac taaggccaga ggtcgcgtcc acaggcaagc cagaatgtct tctggcgcgc
      781 tggacaaccg gagtatccgg cttgggatgg cttgaagatc tcgtcaagga aggtaaggca
      841 atagaccttg gtggcgatgg atatccaaac cgatacccca tcaccttaga cctcgtgctg
      901 cccatccttc atcggggtct gccttccaat gacagtcctg ttgtgatcgg agaggactac
      961 cttgtgcctc cgggttgcaa cggaaaaatt gaagtgaata cggcggagct tgtcgattgt
     1021 tcaaatagcg agtcgttggt cgttgaggca tgggatcaga gctgagcttc gtgcgggttc
     1081 ctctataacg atcgtcgtca tccaaatttt ccatgatgat gcttatcacg ttatttacga
     1141 gcagaagcaa caaggtcgct agcaactcca actctgtgcg caaacagctc ggcgcgtcgt
     1201 tggggactaa ccattttttc aaaagtctcc tgtagatagc tgtccactga gtggaatccg
     1261 atggattttc tgcctccatc gctatcaagc caaagaatga aattgcccac aatcacatct
     1321 gcggtcttga tcgcatcgac gcgcatggca aatcgtcctt tgactcgaaa tcttgcagtt
     1381 tccgtgcagt ccaccatgta gcttacgagt tccgcgggta agctttctgg tggaacgtaa
     1441 gagaggttta gaagattttt taccgtattg gtcgcaaacc tatccatcgg gatcagcaac
     1501 gaagagatga tcgagaacat tgatgctcac ctgtg
//
LOCUS       DATMTI010000565         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_191337, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000565 DATMTI010000000
VERSION     DATMTI010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191337"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1522
                     /locus_tag="VN039_08980"
     CDS             155..1522
                     /locus_tag="VN039_08980"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWV46135.1"
                     /translation="MTIEVPVKLYIDKLLKECRKVSADLALLPGPVKSRVLHAVADRV
                     AAEQDAILAENAKDVETVGKSFEKTDTKDRMRAAVARVRLTADQMKEIVERLHFIADL
                     PDPVGAVTKRQERPDGLQVSRVRMPIGVIGVISERSPLVTVESIALCLKSGNLCVFRG
                     APEWKLTHQAIDARFREAAMENSILNGAWVLIDRPEKEVALELIRSGKHLDAIIVRGG
                     AGLRKTVAEQAKVPVLCDDGGLTHFYVDEDSDISVAQNLAINSKVQQAGASNALDTLL
                     VQQIVARQFLPPLINRLLDQFKVEVHACPKTVALMGQMAMTGHTAIIPATDADWQAQF
                     AGPTLAVKMVEDLDEALAHIRMHGPCLTAGIATTRYESAMRFAREVDAGAVLVNASTR
                     LHAGDSFGMGSDVGLSVGKLHAKGPIGLEQLTCERYVAFGSGQLRLPHPVPETYFDAI
                     MLKTP"
BASE COUNT          334 a          398 c          480 g          312 t
ORIGIN      
        1 catcgcctta ttcgccatct aattgtagca ggttgaaccg cgaggcttgc cggttattta
       61 ctcatgaaaa aaacacgtgg tatagtccga cgccctaaat gtgtgccatt gtctcaggga
      121 gctggatcga ttgcaatgaa tcaatggacc ccatatgacg attgaagttc cagtcaagtt
      181 atacatcgat aagttattga aggagtgcag gaaggtatcg gcggatctgg ccctgcttcc
      241 gggtcccgtg aaatcgaggg tgctgcacgc cgttgccgat cgagttgccg cggagcaaga
      301 cgccattctc gcggaaaacg ccaaagatgt cgagaccgtg gggaagtcct ttgagaagac
      361 ggatacgaaa gaccggatga gggccgccgt ggctcgcgtc cgcctgacgg ccgatcagat
      421 gaaggagatt gtggagcggc tccatttcat tgccgatctg ccggaccctg tcggggccgt
      481 gacgaagcga caggaacgtc cggatgggtt gcaagtctcc cgggtgagga tgccgatcgg
      541 agtgatcggc gtgatctccg aacgaagtcc gctcgtcacc gtggaatcga tcgccctctg
      601 tctgaaatcc ggcaacctct gtgtgtttcg cggggctccg gaatggaagt tgacgcatca
      661 ggcgattgat gcacgctttc gcgaagcggc aatggagaac agcatactca atggtgcgtg
      721 ggtgctcatc gatcgcccag agaaagaagt tgcgctcgaa ctgattcgat cgggcaaaca
      781 cctcgacgcg atcatcgtgc gaggcggagc cggcttacga aagacggttg cggagcaagc
      841 caaggtcccg gttctttgcg atgacggggg gctcacgcat ttttacgtcg atgaagactc
      901 tgatatttcg gtcgcccaaa acttggcgat caactcgaag gtgcagcagg cgggagcctc
      961 caacgcgctg gataccttgc tggtgcagca gattgtggcg cggcaattct tgcccccatt
     1021 gatcaaccgc ctgctggacc aattcaaggt agaagtgcat gcgtgcccga agactgtcgc
     1081 gctcatgggg caaatggcga tgacgggaca taccgcgatc attccggcga cggatgcgga
     1141 ttggcaggct cagttcgccg gtccgaccct ggctgtgaaa atggtcgaag acctcgatga
     1201 agcgctggcc catatcagaa tgcatggtcc ctgcctcacc gccgggatcg ccaccacccg
     1261 ctacgagtcc gccatgcgat tcgccaggga ggtcgatgcc ggagcggtgt tggtgaatgc
     1321 atcaaccaga ctccatgccg gcgatagttt cgggatgggg agcgacgtgg ggctaagcgt
     1381 gggaaaactg catgcaaagg gtccgatcgg tttggagcag ctgacctgtg agagatatgt
     1441 ggcgttcggg tcagggcagc ttcggctccc gcatccggtg ccggaaacgt actttgatgc
     1501 catcatgctg aagacaccct aggc
//
LOCUS       DATMTI010000566         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_105421, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000566 DATMTI010000000
VERSION     DATMTI010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105421"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1585
                     /locus_tag="VN039_08985"
     CDS             <1..>1585
                     /locus_tag="VN039_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HWV46136.1"
                     /translation="GLKHNQIRDEFKRLDKEIIALRGKAIAYECTRKASPPPGRNGAR
                     VDDRTEMVLLNYLMPQQRPRMPVRKILTRAGGSIQALKPCFMMGPQAVAQYLAPGVIK
                     FDLVIMDEASQLKPEEAIGSIARGGQLVVVGDPKQLPPTSFFSRMTQDVDGGDDHFTT
                     TDAESILDVCSSHFRPTRSLRWHYRSQHHSLIAFSNHSFYRGNLVIFPSPYGQGGKLG
                     VRAVYLADAIYENQTNLREAKRVVDAVVEHIATRPNESLGVVTLNIKQRDLIAELLEE
                     RLKSARGADSFRDHWATEGQPLFIKNLENVQGDERDAIIISTTFGKPPGSSAVRQNFG
                     PISRQGGWRRLNVLFTRAKKSIALYTSLRPEDIVMDGTTPDGTKALRNYLEYARTGSL
                     PTIEETDREPDSDFEISVMDMLKMRGYEVTPQLGVAGYRIDIAVKHPDAPGSYLAAIE
                     CDGATYHSALSVRDRDRIRQEILESLGWRGRIWRIWSTDWFRTPRQETEKLINFLEYL
                     RESWKPEHTSGDAWIEEGQG"
BASE COUNT          361 a          465 c          429 g          330 t
ORIGIN      
        1 ctggcctcaa gcataaccag atccgcgacg agttcaagcg gttggacaag gagatcattg
       61 cactgcgtgg aaaggcaatc gcttacgaat gcactcgcaa ggcttctcca ccacccggca
      121 ggaacggtgc ccgcgtcgac gaccgtacgg agatggttct cttgaactac ctcatgcctc
      181 agcaacgccc acgcatgcct gttcggaaaa tccttacccg agcaggcggg tcgattcagg
      241 cgctcaagcc ctgcttcatg atgggaccac aagccgtggc gcaatatctg gctccaggag
      301 tcatcaagtt tgacctcgtc atcatggacg aagcatccca gttgaagccc gaggaggcta
      361 tcggctcaat tgcccgtggt ggccaattgg tggtggttgg tgatccgaag cagttgcccc
      421 cgacgtcatt cttctcccgg atgacgcagg acgttgacgg tggtgacgat cacttcacca
      481 ccacggatgc tgagagcatt ctggatgtct gctcatcgca tttcaggccg actcgctccc
      541 tgcgctggca ttatcgttca cagcatcatt cgttgatcgc tttttcaaac cacagcttct
      601 accgtggcaa tttggtgatc ttcccttcac cgtatggtca gggcggcaag ctcggtgtgc
      661 gtgccgtcta tctggcagat gccatctacg aaaaccaaac caatcttcgc gaagccaagc
      721 gagtcgtgga tgcagttgta gagcacatcg caactcgccc caacgagtct cttggcgtcg
      781 tcacgctgaa catcaagcag cgtgatttga tcgctgaact gctggaagag cgactgaagt
      841 cggctcgcgg cgctgattcc ttccgagacc attgggcaac agaagggcaa cccttgttca
      901 tcaagaacct tgagaacgtc caaggtgatg aacgcgacgc catcatcatc tcaacgactt
      961 ttggcaagcc accagggtct agcgctgttc gtcagaactt tggccccatc agtcggcaag
     1021 gcggctggcg acgactgaac gtgctgttca cccgcgccaa gaagtcaatc gccctgtaca
     1081 catcccttcg tcccgaagac atcgtgatgg atggcactac tccggacggc accaaggcgc
     1141 tacgcaatta cctcgagtac gcccgcacag gctcattgcc gaccatcgag gaaacagacc
     1201 gcgagccaga cagtgatttc gagatttccg tcatggacat gctgaagatg cgtggctatg
     1261 aagtcacccc tcagctcggt gtggcgggat accgcattga catcgccgtc aagcatccag
     1321 acgcaccagg atcgtatctg gcagcaatcg aatgcgacgg ggcaacttac cactcggcct
     1381 tgtcagttcg cgatcgtgac cgcatccgtc aagagatcct agagtctctt ggctggcggg
     1441 ggcgcatttg gcgtatctgg tctacggact ggttccgcac cccacgacaa gaaaccgaga
     1501 agctcatcaa cttccttgag tatttgcgcg agagctggaa gcccgaacac acatcaggag
     1561 atgcctggat cgaagaaggg caagg
//
LOCUS       DATMTI010000567         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_572538, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000567 DATMTI010000000
VERSION     DATMTI010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572538"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..514)
                     /locus_tag="VN039_08990"
     CDS             complement(26..514)
                     /locus_tag="VN039_08990"
                     /inference="COORDINATES: protein motif:HMM:NF028205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HWV46137.1"
                     /translation="MEPVFRDVTFHEGLAYQNVVSSRAEFHYTETAEYLDRFASRISA
                     KGYDPKGPLFYSLNNVPMDEMVDIEMFLPIHQSTFTAEEGLWFHSYFEVSPLLRGVVK
                     GDFENQTERVYAELLATLEANELRINSPFYHLFRREIPPYALVLVGYVDPKEADESLI
                     PA"
     gene            complement(514..957)
                     /locus_tag="VN039_08995"
     CDS             complement(514..957)
                     /locus_tag="VN039_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46138.1"
                     /translation="MKLKPRTLSLNDVVSYRLDIQDGDELLEIIDGTVDYFRHVLVHN
                     GYYTTGPMVFRALPDSTEFTIMTTLGNQVNLVGGSDTGFEFREHVEVETDYFYRHFDV
                     EEPVPYAEIERAVAEAGSRVRNIYHVILDVYGDTLLDLYVEAETR"
     gene            complement(1110..>1812)
                     /locus_tag="VN039_09000"
     CDS             complement(1110..>1812)
                     /locus_tag="VN039_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46139.1"
                     /translation="YKGYEVLSYVTAANVVVIWISAVVLVLSLFKKLIYRFQVFFSSV
                     MAVMAVGLVYTMCSLSLPIATLTRARGELFYAAFFNIAGLVTVGLLAGATVVHVLLLR
                     RRLRVGHSEKRTIGNYVAVSRANISKMLWINFGVVALVVPNVLTSGKFLLNFIGIIAL
                     IVFACVMTSLPVEFAYLAYLKSKDRVYWETPPRGRSKQERLRVAKKAGLWVFGVAAAL
                     TLFWVLAKYLPVWLG"
BASE COUNT          425 a          561 c          479 g          347 t
ORIGIN      
        1 gtcccacccg ccgcggactg accactcagg cggggatgag ggactcgtcc gcttctttcg
       61 gatcgacgta gcccacaaga acgagggcat atggggggat ctcgcgtcga aacagatggt
      121 agaagggaga gttgatccgc agctcgtttg cttcgagggt tgccagaagc tccgcataga
      181 cgcgctcggt ctggttctcg aagtcgcctt tcacgacgcc tctcagcagc ggggagacct
      241 cgaagtagga gtggaaccag agcccctcct cagcggtgaa ggtgctctga tggatgggga
      301 ggaacatctc gatgtcgacc atctcgtcca tcggcacgtt gttgaggctg tagaagagcg
      361 gccctttggg gtcgtagccc ttcgctgaaa tccgtgaggc gaatcggtcg aggtactcgg
      421 ctgtctcggt gtagtggaat tcggcacgcg aactcaccac attctggtag gccagcccct
      481 cgtggaacgt gacgtcacgg aacaccggct ccatcagcgt gtctccgctt cgacgtagag
      541 atcgagcagg gtgtcgccat acacgtcaag gatcacgtga tagatgtttc gcactctcga
      601 ccctgcttca gcgaccgctc tctcgatctc tgcataggga acgggctcct cgacgtcgaa
      661 atgcctgtag aagtaatccg tctccacctc gacatgctcg cgaaattcaa accctgtgtc
      721 agagcctccg accaggttca cctggttgcc gagcgtggtc atgatcgtga actctgttga
      781 atcaggaagc gcacgaaaga ccatcggacc ggtcgtgtag tagccgttgt gcacgagcac
      841 gtgccggaag tagtcgacag ttccgtcgat gatctcaaga agctcatcac catcctgaat
      901 atccagccgg taactcacca cgtcgttcaa gctcagcgtt cgaggtttca gtttcatagc
      961 caaggtcctc tcaccttcat gtcaaagttc ttccctttgt tcgacaagcg acacgcctac
     1021 cgcatcgccg cccaggcgca cgggcccgac atccccccag ccacaggatc gtcacgtgac
     1081 tagtgacgcc gaccgtgcgc gcttttttct cagcccaacc acaccggcag gtacttcgcc
     1141 agaacccaga acagcgtcaa agccgcggcg actccgaaca cccacaagcc ggccttctta
     1201 gccacccgca gccgctcttg cttcgaacgc ccgcgaggag gggtctccca gtagacgcgg
     1261 tctttcgact tcaggtaggc cagatacgcg aactccaccg gaagactcgt catcacacag
     1321 gcaaagacaa tcagcgcgat gatgccgatg aagttcagga gaaacttgcc ggacgtcaac
     1381 acgttcggca ccaccagcgc caccacaccg aagttgatcc acaacatctt ggatatattc
     1441 gcccgggaaa ccgctacata gttgccgatc gtgcgcttct ccgaatgccc cacccgcaac
     1501 ctgcgccgca acaacaacac atgcaccaca gtcgcacccg ccaacaagcc cacagtgacc
     1561 aaaccggcga tgttgaagaa ggctgcatag aagagttctc ctcgtgcccg agtgagtgtc
     1621 gctatgggca gcgaaagcga acacatggtg tagaccaagc cgaccgccat gactgccatg
     1681 acactcgaga agaacacctg aaaccggtag atcaacttct tgaacagcga caggactaag
     1741 acgactgcac ttatccagat gaccacaacg ttcgcagcag tgacatagga caaaacctcg
     1801 taacctttgt at
//
LOCUS       DATMTI010000568         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_488365, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000568 DATMTI010000000
VERSION     DATMTI010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488365"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /gene="pgk"
                     /locus_tag="VN039_09005"
     CDS             <1..686
                     /gene="pgk"
                     /locus_tag="VN039_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWV46140.1"
                     /translation="TITAAMKAPKHPLVAVMGGAKVSDKISVIHELVKVADRMIIGGA
                     MANTFLAYKGKNVGASKVEPDQTAVLDEIYAAAAKKVSHDKVDDFIVLPSDLAVAKTF
                     ANEPRRVVSVDAVAPDDLALDDGDATIERLAGVIADARTVIWNGTLGVAEFPNFAHGS
                     ARLALALATHPDITSIIGGGDTADFVLNWDAKKGGSFTLVSTGGGASLELMAGEKLPG
                     VESLLDARR"
     gene            731..1507
                     /gene="tpiA"
                     /locus_tag="VN039_09010"
     CDS             731..1507
                     /gene="tpiA"
                     /locus_tag="VN039_09010"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWV46141.1"
                     /translation="MRDKKLIAGNWKMNLTMQEASLYLHELSNRVKSHRNVEVVLAPT
                     MLTLQSLSLQVDHRQFKLAAQNLYWRDNGAFTGEVSARQLHGIVQYAIIGHSERRHVF
                     HESDKEVRSKVQAAIRNDIKPILCVGETASERADGETHDVLHDQIVGGLANVTSEEFD
                     RITIAYEPVWAIGTGNNASPRDVKAAEKAIRSQIRHLYGESASESFRLLYGGSVTKES
                     AADYLAIDGIDGLLIGGASLDAEAFSTIVEKAHEMADKKG"
     gene            1802..>2587
                     /locus_tag="VN039_09015"
     CDS             1802..>2587
                     /locus_tag="VN039_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46142.1"
                     /translation="MTIALPASPVNDPTSSTASQPAAEVAQTVEEKSADEAGWPDPLG
                     LQSPAEPTEDKEGESPADSVADTVTSGASSEPLSSPFLPDAKVDKRPLGGPPDINSKL
                     ADEMTRLNDDASHADGTDKEADAVPANEPTDQPTDKPAVLVADKSEAADEPVPASPLP
                     AELDSDLVAIEAGDATTRVIGPDSKEAVIADETVKTDDPSPVPEPPKDLKVEAPSEPQ
                     KTTSVVGSIPQQYKEVSSSNHEVHASIYDEQAEIVPLEHLTKKK"
BASE COUNT          663 a          693 c          741 g          490 t
ORIGIN      
        1 ctacgatcac ggcagccatg aaagcgccaa aacaccctct cgtggctgtt atgggcggcg
       61 caaaagtcag cgacaagata tcggttatcc atgaactggt caaagtcgcc gacaggatga
      121 taatcggtgg ggcgatggcc aacacgttcc ttgcctataa gggcaaaaat gtgggtgcga
      181 gcaaagtcga gcctgatcag acggcggtac ttgacgagat ttatgccgcc gctgcaaaga
      241 aagtcagtca cgacaaggtg gacgacttca tcgttcttcc cagtgatctt gcggtggcca
      301 aaacgtttgc caacgagccg cggcgggtcg tctcggttga tgctgtcgcg ccagacgatc
      361 tggcgcttga tgacggtgac gcgacgatcg aacgcttggc cggtgttatt gccgatgccc
      421 ggacggtcat ctggaacggg acgctcgggg tggcggagtt tccgaatttc gcccatggat
      481 ctgcccggtt ggcgcttgct ctggcgaccc atccggacat cacctcgatc atcggcggcg
      541 gagatacggc cgacttcgtc ctgaactggg acgccaagaa aggcggcagt ttcacactcg
      601 tctcgaccgg aggcggcgcc agcctggaac tgatggcggg agaaaaacta ccgggcgtcg
      661 agagcctgct agacgctcga cggtaaataa gctacactaa aggaaaataa gcataaacga
      721 atttaaacgg atgcgagaca agaaactaat tgcgggtaac tggaaaatga acctcacaat
      781 gcaagaggca agcttgtatt tgcatgagct ttcgaaccgg gtaaagagcc accggaatgt
      841 tgaagtcgtg ttggcaccga ccatgctgac gttgcagtcg ctcagcctgc aggttgacca
      901 tcgccaattc aaattggcgg cgcagaacct gtattggcgt gacaatggcg ctttcacagg
      961 agaagtctcg gcgcgccagc tccatggtat cgtgcagtat gcgatcatcg gccattctga
     1021 acgccgacat gtctttcatg agtccgacaa ggaagtgcgc agcaaggtcc aggcggctat
     1081 ccgaaatgat atcaaaccga tcttgtgcgt tggcgaaacg gccagtgagc gggcagatgg
     1141 cgaaacgcac gacgtcctgc atgatcagat cgtcggcggt ctggccaatg tcaccagcga
     1201 agaattcgac cgcatcacga tcgcctatga accggtctgg gctatcggga ccggtaataa
     1261 tgcgtcaccg cgcgatgtaa aagcggccga aaaagccatt cgtagccaga tccgacatct
     1321 ttacggtgaa agtgccagtg aatcattcag acttctgtat ggtggtagcg tcacgaaaga
     1381 aagtgcagcc gactatctcg cgattgacgg cattgacgga ctgcttatcg gcggtgccag
     1441 tcttgatgcc gaggcgttca gtactattgt agagaaggcg cacgagatgg cggataagaa
     1501 agggtagggc cgcactatgt cagatattga ttttgatgaa ctagacaaag ccgtgagctc
     1561 catcatgtcc tccaagaccg tgggatctac gagcgtggcg gatgcaccgg tcgaagaacc
     1621 accggtgcag gaaacggctc cagagacagc agtcgcatcc gaatctccgg cagccgaaag
     1681 cgaggcgaga ccgtcatctg cggcaccgct cgccaaacgg cgtggtcgtt ttatggatgt
     1741 cgtccatcca tcctcggata tgaaatcggc cacaccgaca aaaccgcaat ctcgcgaagg
     1801 tgtgacgatc gctctgccag cctcacctgt caacgatccg acatcaagta cggcgagcca
     1861 gccagctgcc gaggttgctc agaccgtcga agaaaaatct gctgacgagg ctggatggcc
     1921 ggatccactt gggctccaat cacctgccga gccgactgaa gacaaggagg gcgaatctcc
     1981 tgcagactcc gtagctgata ccgtcacctc tggagcctca tctgagccac tgagttcacc
     2041 attcttaccc gatgcgaaag tggacaagcg tccacttggc ggcccacccg acattaacag
     2101 caaacttgcc gacgagatga cccgtctcaa tgatgatgca tctcatgctg atggaacgga
     2161 caaagaagcc gacgccgtcc cggcgaacga accaactgac caacctactg ataagccggc
     2221 cgtactagtc gccgataagt ccgaagcggc tgatgaaccg gtcccggcat cgccgttgcc
     2281 tgccgagctc gacagcgacc tggtggcgat tgaagcgggt gatgccacaa cccgggtaat
     2341 aggaccagac agcaaagagg ctgtaatagc tgatgaaaca gtaaagaccg acgatccgag
     2401 tccggtgccg gaaccaccga aggatctgaa ggttgaagca ccgagcgagc cccaaaaaac
     2461 aacatcagta gtgggttcta ttccgcagca gtacaaagaa gtctcgagtt ccaatcacga
     2521 agtgcatgct tcgatttatg acgaacaggc tgagatcgtg ccgcttgagc atcttacaaa
     2581 aaagaaa
//
LOCUS       DATMTI010000569         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_275198, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000569 DATMTI010000000
VERSION     DATMTI010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275198"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VN039_09020"
     CDS             complement(<1..1247)
                     /locus_tag="VN039_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV46143.1"
                     /translation="MSTEQRPNAEQTSRYRVDVQHTPRTKHRQQSPHDRLEMLKTCKA
                     NADGDPVLQSAQPPEEDVQARRGTASHAAAGIPAVLKTMEFGWHQMGVVRSLRTYLNI
                     NQKDGFDCPSCAWASPDGKRHRAEFCENGAKAVASEATTRRVTPEFFRRWSVEQLAAQ
                     SDYWLNQQGRLTHPMVLRRGRRHYEPIEWTDAFTLIAQELNRLGSPDEASFYTSGKIT
                     NEPAFLYQLFARQFGTNNLPDCSNMCQEGSGTALSETIGVGKGTVTLKDFELADTILL
                     IGMNPGTNHPRMMTSLWHAKRKGAKMIAINPLPEIGVLRFKNPNPQEYPNPLAMVPAL
                     LGEGAALSDLLLQVRINGDMAVLKGIMKQMLDEEDRHPGQVFDREFINTYTAGFDAVV
                     AELRRISWNEIVEGSGVPLEQIRA"
     gene            complement(1598..2833)
                     /locus_tag="VN039_09025"
     CDS             complement(1598..2833)
                     /locus_tag="VN039_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWV46144.1"
                     /translation="MSLSEWTGRAATVAALAVAALGSYDSFSQVLPADSVKRGEYLAR
                     AGDCISCHTAKGGLPFAGGRRIDTPFGYMLSSNITPDLETGIGNWSADDFYRAMHDGV
                     NRQGQDMYPVMPYDFYTKVTREDVDAIFAYLKTVKPALNTVDTNHLHFPFSLRKTMAV
                     WRELYFTEGTFKPVPEKSEAWNRGAYLVEGFGHCSDCHSPRNLLGGIEKSRDFSGAVV
                     DGWFALDLSSDIATGLGSWSVDDIASYLKTGAYKGKTSTFGPMAEVVQNSTSRMSDAD
                     LKAMAEYLKSIPPETRLRTGRRVPDATRQKGATLYSDNCSSCHQSLGRGMPGIFPPLA
                     GNGAVVASDPADIFNVVLGGIPTQSKYMPMPPFAGQLTDQDIADVTNYIRTSWGNVAQ
                     PNATAAAVAKIRATMPKAK"
BASE COUNT          581 a          865 c          809 g          672 t
ORIGIN      
        1 gcacggatct gctcgagagg aacgccgctt ccctccacga tttcattcca gctaatgcgt
       61 ctgagttcgg ccacgacagc atcaaatccg gccgtatagg tgttgatgaa ctcccgatcg
      121 aagacttgcc ctggatgccg atcctcttcg tccaacatct gtttcatgat gcccttaagc
      181 accgccatgt caccgttgat gcgcacctgc agcaaaagat ccgaaagggc cgcaccttcg
      241 ccaagaaggg cgggaaccat cgccagcggg ttggggtact cctgtgggtt cggattctta
      301 aaacgcaaca ctcctatttc cgggagcgga ttaattgcga tcatctttgc tcccttccgc
      361 ttggcatgcc agagtgaggt catcatgcgg gggtgattgg tgccaggatt catgccgatc
      421 aggaggatcg tatccgcgag ctcgaagtcc ttcagtgtga ctgtcccttt gccaacgcca
      481 atagtttcgg acaaggcagt gccggaccct tcctgacaca tgttcgagca atcaggcaga
      541 ttatttgtgc cgaactggcg ggcgaacaat tggtagagga aagccggctc attggtaatt
      601 ttgccggacg tataaaatga ggcttcatcc ggagagccaa gccggttcaa ttcctgggcg
      661 atgagcgtaa acgcgtctgt ccattcgatc ggctcataat gacgccggcc ccgtctcaga
      721 accatggggt gggtcaggcg accttgctga ttgagccagt agtccgattg cgcagcgagt
      781 tgttcaacgg accaacgtcg gaagaactca ggggtcaccc gacgggtcgt cgcttccgac
      841 gccacagcct tggccccgtt ctcacaaaac tccgcgcgat gccgtttccc gtctgggctc
      901 gcccacgcgc aactcggaca gtcgaaacca tccttttggt ttatgttgag gtacgtcctg
      961 aggctacgca cgacccccat ctgatgccat ccaaactcca tcgttttaag aaccgccggt
     1021 attcccgctg cagcatggga cgctgtcccg cgtctggcct gaacgtcttc ttcagggggt
     1081 tgtgcagact gtaaaacagg atcaccatcg gcattggcct tacatgtttt gagcatttcc
     1141 agccggtcat gaggactctg ctgccggtgc ttggtcctgg gcgtatgttg cacatcaaca
     1201 cgatatcggc tcgtctgctc cgcgttcggg cgctgttctg tactcatggg ttatcctctt
     1261 tcacctacaa tctacgttga tatcgaccca gttggtcaca gattcgttga gtcgcgttgc
     1321 acacgttgaa ccgtctgtcc ttcatgaaac cgaccaacgt catattggcc tccctcacga
     1381 ggtctaccgc aaggctcgac ggggcgacgg gcggaccatt tactggtcac atcaacgctc
     1441 ttggcaccga tatatcctct tctgaagcca gccaggtggc gaaccgccca cacgctcacc
     1501 aaacgaacgg tccgactcat cacgagcctt attcctcacg cttctgccca aacacacatt
     1561 caccacacga tgtgcattct ccacaagtac ctccggatca cttcgccttc ggcattgtcg
     1621 cgcgtatttt ggccactgct gctgcggtgg cgttcggctg cgcgacgttg ccccagctcg
     1681 tccggatgta attagtgacg tcggcgatgt cctgatcggt tagctggcct gcgaacggcg
     1741 gcatcggcat atacttgctt tgcgtcggta tgccgcccag cacgacattg aagatatccg
     1801 ctggatcgga cgcgaccacg gcgccgttac cagcaagcgg cggaaagatg cccggcatac
     1861 cgcgcccaag cgattgatgg caggagctgc aattatccga atacagtgtg gctccctttt
     1921 gccgcgttgc gtccgggacc cgtcgtccag tgcgcagcct tgtctctgga ggaatggact
     1981 tcaggtactc ggccatcgct ttgaggtcag cgtcggacat gcggctcgtg ctgttctgga
     2041 cgacttcagc catcggcccg aacgtcgatg ttttgccctt atatgcgccg gttttcaagt
     2101 atgacgcaat gtcgtcgacg ctccaagagc ccaatccggt cgcgatgtca gaactgaggt
     2161 ccagcgcgaa ccatccgtcg acgacggcgc cggagaaatc cctactcttt tcgatgccgc
     2221 ccaagagatt cctcggcgaa tggcagtcgc tgcagtggcc gaacccctcg accaggtagg
     2281 cgcctcgatt ccatgcctcg gacttttccg gtaccggctt aaatgtgccc tcagtaaagt
     2341 aaagctcgcg ccagacggcc atcgttttcc gcaagctgaa cgggaaatgc agatggtttg
     2401 tgtcgacggt gttcagcgct ggcttgacgg tcttcaggta cgcaaagatt gcgtcgacgt
     2461 cctcgcgtgt caccttcgtg tagaaatcgt acggcatgac cgggtacatg tcttgtccct
     2521 gcctgttcac gccatcgtgc attgcccggt agaagtcgtc ggccgaccag ttgccgatgc
     2581 ccgtttctag atcgggcgtg atattggacg aaagcatata gccgaacggc gtgtcgatgc
     2641 gccggccgcc ggcgaagggt agaccgcctt ttgcggtgtg gcaggagatg caatcgccgg
     2701 cacgcgcgag gtactcgcct cgcttgaccg aatccgccgg caacacctgg gaaaaactgt
     2761 cgtatgatcc gagtgcggca acggcaagtg cggcgactgt cgcggcacga cctgtccatt
     2821 cagatagtga catgtttgtt tgttctcctc tccggttcca cggtgggcat ccatgccgac
     2881 aaggtcccaa tgtatgattg caagaccttg agcatggggt agagagt
//
LOCUS       DATMTI010000570         5618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_21257, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000570 DATMTI010000000
VERSION     DATMTI010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21257"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VN039_09030"
     CDS             <1..341
                     /locus_tag="VN039_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46145.1"
                     /translation="GASFGLENMAITMWDHLIPGLTHLKLTRCPKMDLTYFTFHRQLE
                     VTHEEAMEQAVAAVQGTDETRMSKQDEDDFRSGVDAVLDYLEGFWAGLEQRRRSPGNT
                     WTGLAQRRAS"
     gene            395..2110
                     /locus_tag="VN039_09035"
     CDS             395..2110
                     /locus_tag="VN039_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWV46146.1"
                     /translation="MQQTWISRYDSGVPASINYPAWTVSDLLQHSSERFANSPALMFY
                     GTCITYGELDGLATRFALGLRQLGVGQGDRVAIMLPNIPQAVIAYYGILKAGAVVVPT
                     NPLYVEREIQTQLTDAGAETMVVLDLLYLRVRAVRDATTLPKRIIVTGVQDYFPLFKK
                     LLYPIKARLAKRWVSVEKSPPVYDFLELLCINSVNGADHSAGLPLVRPTDLAQLQYTG
                     GTTGTAKGVMLTHRNVVVNTLQGRYWSPDFREGQEIFLGAIPFFHCYGQATCQNLAIA
                     TGCPIVLLPRFHADEVIKAIHTHRITMFSGVPMMYSMIVEHPDAGRYDLRSLRVCLSG
                     ASSLPTDVQERFENLTGARISEGYGLTEAGPTTHCNPLHGEHPRGSMGLPFPDTEARV
                     VDPDAGLEEVPKGETGELIVRGPQVMRGYWSREDETRAVLRGGWLHTGDLVRRDDRGY
                     FYFVDRKKDIIKSRGETVYPREVEEVLRQHPAVSEAAVIGVADHDYGEIIKAYVVAKP
                     ESFVTEQALITHCAGLLARYKIPSIVEFRQELPRTVIGKVLRRVMRAEAAQASAEEQL
                     QRQAV"
     gene            2131..3321
                     /locus_tag="VN039_09040"
     CDS             2131..3321
                     /locus_tag="VN039_09040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWV46147.1"
                     /translation="MRDVVIVAGVRTPIGNFGGALKDVPPQRLGELVVREVLARGGLD
                     SRLIEEVIFGCVGQPSDAFNIARVIALQAGLPLTTPAYTVQRNCSSGLQPFVNAFQNL
                     QSHDADVQIVGGVESMSRAPFLSRDMRWGKRLRHAEFIDSIWEGLTDPVCGQLMGRTA
                     ETLAEELGITREEQDRFAVESHHRAFKAVREGRLKEEIHPVTVSKSVGGREVAPLVVA
                     QDEGPNVALTEPQLSLYPPLFKEGGTVTAGNSCPLNDGAAAAIVMSAERARDLGLQPL
                     GRIRSYVFVGVDPAHMGIGPVQALPAALKRAGIQLTDLDLIEVNEAFAAQYLAVERQL
                     GLKRDLVNVNGGAIALGHPVGMSGTRLVITMLCEMRRRGSALGAVTMCVGGGQGAAMI
                     LEQV"
     gene            3412..4635
                     /locus_tag="VN039_09045"
     CDS             3412..4635
                     /locus_tag="VN039_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HWV46148.1"
                     /translation="MYIYKVGVVGAGTMGAQIAEVVSYAGVPVVLADVNLTSAQRGVE
                     AVRAIYQARVDKGKMSPEQLEQKMLLVTPSSGLEEFKGVDLAIEAISEDMKLKELMFR
                     ELDQVCPRSAILASNTSALSLSGLGAATKRPDKVLGLHFFNPAYVMRLVEVVPGLATS
                     SQTIDDAVNFSQGVGKLPIVVKECAGFLVNRVLMAYVNEAVRALQEGAATLQEIDQDM
                     VAFGMPVGPLALLDAVGLDISFEVARILYRSYGPRMTPAPLLEAMLKAGRLGVKSGHG
                     FYDYVGGKEGDREQEMEQLLNKVRSRGERSVTRWNRSRLLLAMTNEAVIALQEGVASA
                     TDIDLAMMAGIGYPKEKEGPLHFADQLGIDQVLRELEDFAQTLGPRFWPAPMLRRMVN
                     AGFTGQTAGRGFFTY"
     gene            4679..5467
                     /locus_tag="VN039_09050"
     CDS             4679..5467
                     /locus_tag="VN039_09050"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWV46149.1"
                     /translation="MTAIAGTMHNCKIEEGVALVTLDHPPVNALTPELLAELNSTFDS
                     LAKDEAVKAVVLTGTGRFFVAGADIRVLASIPSSVEGEAMARRGQMILNRIETFEKPV
                     IAAINGACLGGGLEVAMCCHIRLAAEGARLGQPEINLGIMPGFGGTQRLPRLIGRSKA
                     LELILIGEPISAREAMNLGLVSQVVPVDDLLRQAQGLARTMAAKSQVALRASLRAIHQ
                     GSALNLSDGLDLEARLFGTLCDTEDRKEGTAAFLEKRQPRFKNR"
BASE COUNT         1195 a         1538 c         1729 g         1156 t
ORIGIN      
        1 tgggagcatc ctttggactc gagaacatgg ccatcaccat gtgggatcat ttgattccgg
       61 gtctgaccca cttgaaactc acgcgttgtc ccaagatgga cctcacctat ttcacgtttc
      121 accggcaact tgaagtcact cacgaagaag caatggaaca ggcggtagcg gccgtgcaag
      181 gaacggatga gaccagaatg tccaaacagg atgaggatga ttttcgttcc ggcgtcgatg
      241 ccgtgctcga ctaccttgaa gggttttggg caggtttgga gcaacggagg aggagccctg
      301 gcaacacgtg gaccggctta gctcagcggc gtgcgagtta gcgccgtgag cgatacggct
      361 ggcttgcatg tgcctgagag tggaggaggg tcctatgcaa cagacttgga tcagccgata
      421 tgattccggg gtgcccgcaa gcatcaacta cccggcatgg actgtatcgg acctcctgca
      481 acattcatcg gagcgtttcg cgaactcccc tgcactgatg ttttatggta cctgcatcac
      541 ttatggagaa ctggacggcc tcgcgactcg atttgcgctt gggctgcgac aactcggcgt
      601 cggtcaaggc gatcgggtcg ccatcatgct tccaaatatt ccgcaggcgg tcatcgccta
      661 ttacggaatt ctgaaagccg gcgccgtcgt cgtgccgacc aatccgctct acgttgaacg
      721 agaaattcag acacaactga ccgatgcagg ggcggaaacg atggtggtcc ttgacctact
      781 ctatcttcgc gttcgagcgg tgagagacgc gacgacgctc cccaagcgga tcatcgtcac
      841 cggcgttcag gactatttcc ccctcttcaa gaaactcctc tatcccatca aagctcggtt
      901 agccaaacgt tgggtttccg tcgaaaaatc tccgcccgtc tatgacttcc tcgaactcct
      961 atgcatcaac tctgtaaacg gtgcagatca ttccgccggt ctgcctctgg tacgaccgac
     1021 cgatctcgca caactgcaat ataccggtgg gacgacggga acagccaaag gcgtgatgct
     1081 cacgcaccgt aatgtggtcg tcaatacgct gcagggccga tactggtcac ctgattttcg
     1141 tgaaggtcag gaaatctttc tgggtgcaat tccatttttc cactgctatg gtcaggccac
     1201 ctgtcagaac ctagccatcg cgacgggctg tccaatagtc ctcctccctc ggttccatgc
     1261 ggacgaagtt atcaaggcga tccataccca tcgcattacc atgttttcgg gcgtgcccat
     1321 gatgtattcg atgatcgtcg agcatccgga tgctggccgc tacgacttac gctcgttgcg
     1381 tgtgtgtttg agtggggcca gctcgcttcc tacagatgtt caggaacggt tcgaaaatct
     1441 caccggggca aggatttcag aggggtatgg cctgaccgaa gccggtccca cgactcattg
     1501 caatccgttg cacggagaac atccacgagg ctcgatggga cttcccttcc ccgataccga
     1561 ggcgcgggtg gtggaccctg atgctggcct ggaggaggta ccgaagggtg aaacaggcga
     1621 attgatcgtg cgcgggccac aggtgatgcg gggatactgg agcagggaag acgaaacacg
     1681 cgccgtgctt cgtggcggat ggctgcacac cggcgacctc gtccgccggg acgatcgcgg
     1741 atacttctac tttgtcgatc gcaagaaaga catcatcaag tcgcgaggag aaacggtgta
     1801 tccgcgagaa gtcgaagaag tcttgcgcca acacccggcg gtgagcgaag ccgccgtgat
     1861 aggcgttgcg gaccacgact acggtgagat catcaaagcc tatgtcgtgg caaaaccgga
     1921 atcgttcgtc accgagcagg cattgatcac gcactgcgcc ggcttgctgg cgcgatacaa
     1981 gatcccgtcg atcgtggaat ttcgtcagga gttgccgcga acggtcatcg gtaaggtgct
     2041 gcgcagggta atgcgtgccg aggctgctca agcgagtgcc gaggaacagc ttcaacggca
     2101 ggctgtgtga acgttcggat ggaggaccct atgagagacg tcgtgatcgt ggccggtgtg
     2161 cgaacgccga tcggcaattt cggcggtgcg ttgaaggacg tcccgcctca acggctcggg
     2221 gaactggtcg tccgcgaagt cctcgcacgg ggcggtcttg attccaggct gatcgaggag
     2281 gtcatctttg gctgcgtcgg acagccgagt gacgcattca acatcgcccg cgtcattgcc
     2341 cttcaagccg ggcttcccct cacgactccg gcgtacaccg tgcagcgcaa ctgttcctcg
     2401 ggactccaac cgtttgtcaa tgccttccag aatctccaga gtcacgatgc ggacgtgcag
     2461 atcgtcggcg gcgtcgaaag catgagtcgc gcgccgtttc tgtctcggga catgcgatgg
     2521 ggcaagcggc ttcgacatgc tgaattcatc gacagcattt gggaaggatt gacggaccca
     2581 gtttgcggcc agctcatggg acggacagcg gagaccttgg ccgaagagtt ggggatcaca
     2641 cgggaagaac aggatcggtt tgcggtcgag agccaccacc gtgcattcaa ggcggttcgc
     2701 gagggcaggc tgaaagagga aatccaccct gtcacagtct ccaaatcggt cggggggcga
     2761 gaggttgccc cgctcgtggt cgcacaagac gaaggtccga acgtcgctct caccgaaccg
     2821 caactgtccc tctacccacc tctcttcaaa gagggaggca ccgtgacggc gggaaacagt
     2881 tgcccgctga acgacggggc agccgcagcg atcgtcatgt cggctgagcg tgcgcgagac
     2941 cttggcttgc agcctcttgg ccgcatcaga agttatgtct ttgtaggagt cgatccagcg
     3001 catatgggca tcggaccggt gcaggcgctg ccggcggcgc ttaagcgagc aggcatccaa
     3061 ttgacggacc tcgacttgat cgaggtgaac gaggcgttcg ccgctcagta cctggccgtc
     3121 gaacggcagt tgggtctcaa acgtgacctc gtcaacgtca acggcggtgc catcgcatta
     3181 ggccaccctg tcggaatgtc cggcacaaga ctcgtcatca cgatgctctg tgaaatgcgc
     3241 cgacgcggtt cagcgttggg cgcggtgacg atgtgcgtgg gcggcggaca aggagcagca
     3301 atgatcttgg agcaagtgta accaaccggt catcagtcgt cggcaactct gtcaccgacc
     3361 actggcggga tgctgaaggc tgagaactga acgtatggag cggagccagc gatgtacatc
     3421 tacaaagtcg gcgtcgtcgg ggctggtacc atgggagcgc aaattgctga agtggtgagc
     3481 tatgccggcg ttccggtcgt actggccgat gtgaatctca cgtcagcgca acggggtgtc
     3541 gaggccgtac gcgcaatcta tcaggcgcgt gtggacaagg gaaagatgag cccggagcaa
     3601 ttggagcaga aaatgttgct cgtgacccca tcgtcgggtc tggaagagtt caagggtgtc
     3661 gatctggcga tcgaggcgat ttcggaggac atgaaactta aggagctgat gttccgcgag
     3721 ttggatcagg tctgtccgcg aagcgcgatc ttggcgagta acacgtcggc gttgtctctt
     3781 tccgggctcg gagcagcgac gaaacgccct gacaaggtac tcggcctcca tttcttcaat
     3841 ccggcttatg tcatgcggct cgtagaggtc gtaccggggt tggccacctc ttcgcaaacc
     3901 atcgacgatg cggtgaactt ctcgcaaggc gtcggaaaac tgccgattgt ggtgaaggag
     3961 tgcgcagggt tcctggtcaa tcgtgtcctg atggcctatg taaacgaagc cgttcgtgcc
     4021 ttgcaagaag gagccgccac attgcaggaa atcgatcagg atatggtcgc cttcggcatg
     4081 ccggtcgggc cgctcgctct tctcgatgcg gtgggccttg acatctcgtt cgaggtcgca
     4141 cgcatcttgt accgcagcta tgggccccgc atgacgccgg ctcccctgct tgaagccatg
     4201 ttgaaggctg gccgtctagg agtcaaatcg ggtcatgggt tctacgacta tgtcggcgga
     4261 aaggaaggcg atcgcgagca ggagatggaa caacttctca acaaagtccg atcgcgcggt
     4321 gaacgcagcg tgactcgatg gaatcgctca cgattgctcc tggccatgac caatgaggcc
     4381 gttattgcgc tccaagaagg agtcgcctcg gcaacggata tcgatctagc gatgatggcc
     4441 ggaatcggct accccaagga aaaggaggga ccgctgcatt tcgcggatca gctgggcatt
     4501 gatcaagttc tgcgcgagct tgaggatttc gcccaaacat tgggaccacg cttttggccc
     4561 gcgccgatgt tgcggcggat ggtcaatgcc ggatttacag gtcagacagc tggacgaggc
     4621 tttttcacat actgaagtcg ccggatcggc ctccgaattg gcaaggaagg aacgcgtcat
     4681 gactgctata gcgggaacca tgcacaattg caagattgaa gaaggcgtcg ccctcgtgac
     4741 cctcgatcat ccccccgtca acgccctcac acctgaacta ctcgcagagt tgaactccac
     4801 gttcgactct ctcgcgaagg atgaagccgt aaaggcggtg gtgctcacgg gcacgggccg
     4861 attttttgtg gcaggggccg atatccgagt acttgcctcc attccgtcgt cagttgaagg
     4921 cgaggcaatg gcccgccggg ggcagatgat cttgaacagg attgagacgt ttgaaaaacc
     4981 ggtcatcgcg gcgatcaacg gtgcttgttt gggcggaggg ttggaagtgg ccatgtgttg
     5041 ccacatccgc ctcgcagccg aaggcgccag acttggccaa ccggaaatca acctgggcat
     5101 catgccggga ttcggcggga ctcaacgtct tcctcggttg atcggccgat ccaaggcctt
     5161 ggaattgatc ctgataggcg agcctatctc agctcgagag gccatgaatc taggtttggt
     5221 gtctcaggtg gtacccgttg acgacctcct gcgacaagcg caaggactgg cgcgcacgat
     5281 ggctgcgaaa agccaagtcg cgctccgcgc gtcgcttcgg gcgatccatc aggggagtgc
     5341 gctgaatctt tcggatggct tggatctgga agcccggctg ttcggaacgt tgtgcgatac
     5401 cgaagatcgg aaagaaggaa cggcggcctt cttggagaaa cggcagcctc gattcaagaa
     5461 ccgttagtct ccagaaagac agcgaggaga catgatgcag gctcaacgtt cgaccaaatt
     5521 ggccttggct ctgacaggag gcggcttcac gggctatctc ttcgaagtcg gcgccttgac
     5581 cgggttggac gatcttctcg gcgagtcgtt caccagca
//
LOCUS       DATMTI010000571         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_614873, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000571 DATMTI010000000
VERSION     DATMTI010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614873"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VN039_09055"
     CDS             <1..228
                     /locus_tag="VN039_09055"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV46150.1"
                     /translation="ESASALPLLEPLVAADPTNVVAHFRLSTAYRKLKRPADAKREMD
                     EYQKYKDIKEKMRAVYKEMRLDTPQDAPDNK"
     gene            265..>1529
                     /locus_tag="VN039_09060"
     CDS             265..>1529
                     /locus_tag="VN039_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWV46151.1"
                     /translation="MRRGNPSYSAAALACCIGLILYTGLLQAQVAKPNLSSEHSGASE
                     HVPQLVDITASTGIHFEHLSSPEQKYIVESMSGGVALLDYDGDGWLDIYFTNAPSVSM
                     ALAGKKARGALFHNNHDGTFTDVTEKAGVGYPCWAMGAAVGDYNNDGRPDLIISCFGG
                     VVLYRNNGDGTFTDVTRATGLDKDTGWATGVTFGDYDGDGNSDLFVPHYVDLDMNDLP
                     KFGSSKTCQYHGVAVQCGPRGLKGSPDSLYRNNGDGTFTDVSKQAGVDDPNHFFGLGA
                     VWSDFDNDGKLDLFVANDGEPNYLYHNEGNGHFKEMAYDAGVAVSEDGTEQANMGLAL
                     GDYLNVGRMSLVVSHFSDEYAAMFRNDGGMTFSDVSRASGISRSSSPYVGWGDAFLDL
                     DNSGWKDLILVNGHVYPQVDSVKMGTRYK"
BASE COUNT          345 a          413 c          453 g          318 t
ORIGIN      
        1 gaatctgcct ccgcgttgcc gctgctggag ccactggttg cggccgatcc caccaatgtc
       61 gtcgcccact ttcggctcag cacggcctat cgcaaattga agcgacccgc ggacgcgaag
      121 cgagagatgg acgagtatca gaagtacaaa gacattaagg aaaaaatgcg agcggtttat
      181 aaggagatgc ggctcgatac cccgcaggac gcgccggata ataagtagat ggagtcattg
      241 cggaatcagc ttcgggcctg gagaatgcgc cggggaaatc cttcgtacag cgccgctgcg
      301 ctggcatgtt gcatcggcct gatcttgtat acgggacttc ttcaagccca ggttgcgaag
      361 ccaaatcttt ctagcgagca ttccggggcc agcgagcatg tgccgcagtt ggtggacatt
      421 acggcctcga ccggcattca ctttgagcat ctctccagcc cggagcagaa gtacatcgtc
      481 gagtcgatga gcggcggcgt tgctctgctc gattacgacg gcgatggctg gctcgatatt
      541 tacttcacca atgcgccgag tgtttccatg gcgctcgctg ggaaaaaagc aagaggcgct
      601 ctctttcata acaaccacga cggtacattc acggacgtca cagagaaagc tggcgtcggt
      661 tatccctgct gggccatggg cgcggcagta ggagactaca acaacgatgg acgccctgat
      721 ctgatcatta gctgcttcgg cggcgtggtg ctctatcgca acaacggcga tggcaccttc
      781 accgacgtca ccagggccac ggggctggac aaagacacag gctgggctac cggagttacc
      841 tttggcgatt atgacggcga cggcaactcc gatctgttcg tcccgcacta tgtcgatttg
      901 gacatgaacg atctgcccaa gttcggatcc agcaagacgt gtcaatatca cggagtcgcg
      961 gttcagtgcg ggccgcgcgg gctcaagggc tctcccgaca gcttgtatcg caataatggc
     1021 gatggcactt tcaccgatgt gtcaaagcag gcaggcgtcg acgacccaaa tcatttcttt
     1081 ggcctgggag ccgtctggtc cgacttcgat aacgatggaa aactcgatct cttcgtcgcg
     1141 aacgatggcg agccgaacta tctctaccac aacgaaggca atgggcactt caaggagatg
     1201 gcttacgatg ccggtgtggc cgtaagcgaa gacggaacag agcaagcgaa catgggactt
     1261 gcccttggag actatctgaa tgtcggtcgg atgagccttg tagtgagcca cttcagtgac
     1321 gaatacgccg caatgtttcg caacgatgga ggaatgacct tctccgacgt ctcccgcgcc
     1381 tccggaatct ctcgttcgag ttcgccgtat gtcggctggg gcgatgcgtt tctggatctc
     1441 gataactccg gctggaagga tctgatcctg gtgaacggtc atgtctatcc gcaggtcgac
     1501 agcgtgaaga tggggacgag atacaagga
//
LOCUS       DATMTI010000572         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_785342, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000572 DATMTI010000000
VERSION     DATMTI010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785342"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..621)
                     /gene="tsaE"
                     /locus_tag="VN039_09065"
     CDS             complement(79..621)
                     /gene="tsaE"
                     /locus_tag="VN039_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWV46152.1"
                     /translation="MATSISSLDLILSSPGETESFGHAIGRLLRGGEVLALIGELGAG
                     KTALVRGIVAGLGAPAASVTSPTFLLVHEYQGRLPVVHIDLYRLQRLEEIELIGLSDY
                     LTDEVVVAIEWADRFPQWLPDDRLEVQVAHRTRTTRKAHVEARGPRSRLLLAQIKKIV
                     HPIPRSVRPRLRGGRRTSPR"
     gene            complement(632..2230)
                     /locus_tag="VN039_09070"
     CDS             complement(632..2230)
                     /locus_tag="VN039_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWV46153.1"
                     /translation="MTKIITAAQMQELDRRTISEAHIPATTLMERAGSGVVSCLEQRL
                     GSVRGKTVTVVCGKGNNGGDGFVAARLFRRRHAKVRVLAMAPTSELSRDAAAMYRQFV
                     RGAGKSSVYLYTSRVQAQALLHDSDIIIDALLGTGLSSTVTGRYAEAIDGINGTSRPV
                     VAVDLPSGLHADTGAILGRAVRASLTVTFGLPKLGLYQNYGIDLAGEVAVVDIGIPPA
                     YLDAVESRTTLMTQHVVRAYLPKRQPSSHKGTFGHAGIIAGSVGKTGAAAMAARAALR
                     IGAGLVTVAVPTSVNDVLESKLLEVMTVPMPETKARTFARTALDRLVAFMSARTAIAI
                     GPGLSTHPETVELVQALTKLLDRPAVLDADALNALTGRTALLASCKTPPIITPHPGEM
                     ARLEADATPQTVNSDRIGFASRFARERGLFVVLKGARTVVARPDGAVAVCPTGNPGMA
                     TAGTGDVLTGMMVGLLAQGIPSWEAACAATYLHGTAGDLAAVRTGQAGLIAGDVIEEI
                     PHALRLIHQASSKDKPIEIWNALL"
     gene            complement(2282..2995)
                     /locus_tag="VN039_09075"
     CDS             complement(2282..2995)
                     /locus_tag="VN039_09075"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HWV46154.1"
                     /translation="MARLGVNIDHVATLRQARGGADPDPLAAAVLVELAGADGLVVHL
                     REDRRHIQDRDLHLLREIARTKLDLEMAADGEMAKIALNIKPDLVTLVPEKRQELTTE
                     GGLDVVGQRDRIQGIVTLLHNGGIPVSVFVEPDLNQIRAAHKISADFVELHTGRYANA
                     TRSKEADSEFEAITHAAKLAYKLGIGVNAGHGLNYRNIKRLTHIPEIVEYNIGHSIIA
                     RAVLVGLVQAVKEMKALLG"
     gene            3284..>3826
                     /locus_tag="VN039_09080"
     CDS             3284..>3826
                     /locus_tag="VN039_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWV46155.1"
                     /translation="MGLGEGFYRIKRLPPYVFAQVQSLKLEARQRGEDIIDFGMGNPD
                     QPTPPHIVEKMAEAARKAKNHRYSASRGITKLRHAICGWYKRSYDVDLDPETEAIVTI
                     GSKEGLAHLALAMIGPGDVVLTPTPTYPIHMYSFIIAGGEVRGIELRQDSDFFEDLTR
                     VYRQTFPRPKILVINFPHNPT"
BASE COUNT          824 a         1098 c         1097 g          807 t
ORIGIN      
        1 gtgtgacttc ctgctcctgg ttttgaagaa agaaggccaa cgaaaggagc aaaagagtgc
       61 cgaccagaat cagccgtgtc atcgcggcga agtccttctg cctcctcgga ggcgtggtcg
      121 gactgatcgc gggattggat gcacaatttt cttgatttga gcaagaagta aacgagagcg
      181 tggacctcgc gcttcgacat gggcttttct ggtcgttctg gtccgatggg caacctgcac
      241 ttccagccgg tcgtccggga gccattgggg aaaccggtct gcccactcaa tggcaacgac
      301 cacctcgtcc gtgaggtaat ccgatagtcc gatcaactcg atttcttcaa gtctctgcaa
      361 ccggtacaaa tcaatatgaa caaccggaag ccgcccctga tattcatgga caagcaggaa
      421 cgtcggactc gtgacggatg cagccggtgc gccgagcccg gcaacgatcc cgcggaccag
      481 tgctgtctta ccggcaccca attcaccgat caaggcgagc acctctcccc cgcgaagcag
      541 ccggccaatg gcatgtccga acgattctgt ctcaccggga gatgagagga taaggtcaag
      601 ggagctgata gatgtagcca tgggagcgtc ttcacaggag agcattccat atttcaatgg
      661 gtttgtcttt tgatgaagcc tgatgtatga gcctgagggc atggggaatc tcctcgatca
      721 cgtctcccgc gatcaacccg gcttgccccg ttctgactgc agccaaatcg ccggcagttc
      781 catgcagata cgtcgcggca caggcagcct cccaggaggg tatgccttgg gctaagagtc
      841 ccaccatcat gccggttaac acatcacctg ttcctgccgt cgccattcca ggattgccgg
      901 tagggcaaac agcgacggct ccatccggtc gggcgacgac ggtccgagcc cctttcaaga
      961 cgacgaacag gcctcgctct cgcgcgaagc gggaggcaaa accgatccga tcgctgttga
     1021 cggtctgagg cgttgcgtca gcctccagtc gtgccatctc tcccggatgg ggtgtgatga
     1081 tcggcggtgt tttgcaagac gccaggaggg cggtccgccc tgtcagcgcg ttcagggcgt
     1141 ctgcatccag gacagccggc cggtcaagca gtttcgtcaa ggcctggacg agttcaactg
     1201 tttcgggatg agtggataat cccggaccga tggcgatagc ggttctggcc gacatgaagg
     1261 cgacgagccg atccaatgcg gtccgtgcaa aggtgcgcgc tttggtttca ggcattggaa
     1321 ccgtcatcac ttccaacagc tttgattcca agacgtcgtt gacacttgtc gggactgcaa
     1381 cagtcacgag gcccgcgccg atacgaaggg ccgctcgtgc agccatggcg gcggccccgg
     1441 tttttccaac ggacccggca ataatgccgg catggccgaa tgtacccttg tgactcgatg
     1501 gttgccgttt cggcaggtat gctcgtacca cgtgttgtgt catcaaggtc gttcggctct
     1561 cgaccgcgtc aaggtaggcc ggcggaattc cgatgtccac gacggccacc tctccagcca
     1621 ggtcgatccc atagttctga tagaggccca actttggaag cccaaacgtc acggtgagag
     1681 aggcacgaac ggctcgaccc aggatagcgc cggtatcagc atggaggccc gatggaagat
     1741 caaccgccac cacgggacga ctggttccgt ttatgccgtc aatggcttcg gcatagcgtc
     1801 cggtcacagt cgaggagagt cccgtcccga ggagggcatc gatgatgata tcactgtcat
     1861 gcaaaagtgc ttgggcctga actcgggacg tatagaggta aacggaagat ttccctgcgc
     1921 cacgaacaaa ttgcctatac atcgctgccg catcgcggct cagttcggag gtgggtgcca
     1981 tggcgagtac gcgcaccttc gcatggcgcc gacggaatag gcgagcagcg acgaacccat
     2041 ctcccccatt gttgcctttg ccgcacacaa ccgtgaccgt cttgccgcgc acagatccca
     2101 agcgttgctc aaggcacgat acgacgccgg acccggcccg ctccatcagc gtcgtcgccg
     2161 ggatgtgcgc ctccgaaatc gttcggcggt cgagctcctg catctgcgcg gcggtgatga
     2221 tcttggtcat gatgaaccga aggtggtgaa cgggcggact ctgtgagtcg acgacattcg
     2281 attagccgag caaggccttc atctctttca cggcctgtac gagaccgacg aggaccgccc
     2341 gtgcgatgat gctgtgcccg atgttgtact ccacgatttc gggaatatgc gtgaggcgct
     2401 tgatgttgcg gtaattcagt ccatgcccgg cattgacccc gatcccgagc ttgtaggcca
     2461 gcttggcggc gtgggtaatg gcttcaaatt ccgaatcggc ttctttggag cgtgtggcat
     2521 tcgcgtaacg accggtatgc agctccacga aatcagcgga aattttgtgc gcggccctga
     2581 tctgattcag gtccggctcg acaaagacgc tgacgggaat tcctccgtta tgcaacagag
     2641 tcacaatgcc ttgaattcga tcgcgttgtc ccacgacatc aaggccacct tccgtggtga
     2701 gctcctgccg cttttccggg accaacgtga caaggtcggg cttgatgttc aaggcgattt
     2761 tcgccatttc cccatcggcc gccatttcga ggtcgagctt tgtccgggct atttcgcgaa
     2821 ggagatgaag gtctcggtct tggatatggc gtcggtcttc ccggagatga acgaccaaac
     2881 cgtctgctcc tgccagctcg accaggacag ccgccgccaa aggatccgga tcggctcctc
     2941 cacgtgcctg ccgcaaggtc gcgacgtggt cgatattcac tccaagccgg gccatccctc
     3001 ctcctttatt caaatcagcc tgatcatgaa cgcgtgaggc aagtagactc tgcaatcaaa
     3061 cgctattagt agcagaaagg aacgagaggg agcaagaacg ggttcgccga taggcgaagg
     3121 acggggatga gaagtccctg cccttggtaa cttcagaaac ccaggaatga ggcaaattga
     3181 ctcgaccaaa gccctccatt taaaatacgc gctttccaca tcccttcgct catttatgca
     3241 atgagttggt ttggggaata tctaacaaca atcaaaggaa gtcatggggt tgggcgaagg
     3301 tttttataga atcaagcgac tcccaccgta tgtcttcgcg caggttcaat ccctcaagct
     3361 tgaggctcgg caacgagggg aagacattat tgattttggg atgggcaatc ccgatcaacc
     3421 gaccccgcct catatcgtcg agaagatggc cgaggctgcc cgcaaggcaa agaatcatcg
     3481 ttactcggcc tcgcgcggca tcacgaaact gcggcatgcg atttgtgggt ggtacaagcg
     3541 aagctacgat gttgacctgg atccggagac cgaagccatt gtcaccatcg gatcaaaaga
     3601 aggcctggcc catctggcct tggccatgat cggcccgggc gatgtggtct tgaccccgac
     3661 ccctacctat cctattcata tgtacagctt catcattgcc ggtggtgagg tccgtggcat
     3721 cgaactccgc caggatagcg actttttcga agatctgacg cgcgtctatc ggcagacgtt
     3781 tccgagaccg aagattctcg tgattaattt tccccacaat cccacc
//
LOCUS       DATMTI010000573         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_234656, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000573 DATMTI010000000
VERSION     DATMTI010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234656"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VN039_09085"
     CDS             <1..332
                     /locus_tag="VN039_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV46156.1"
                     /translation="MTTETFGFVIASKRREKGYSQKELAERIKLPGDAGSAKSISPQY
                     LNDIEHNKRSPSSPELVAQFSRVLGLNADYLAFLADRWPESLRRQIKSESDFASFVTA
                     FRRTHGT"
     gene            329..1051
                     /locus_tag="VN039_09090"
     CDS             329..1051
                     /locus_tag="VN039_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46157.1"
                     /translation="MRLIPDPTGRFTQRPFYEAGELDVECEKVMSQFLRSIHGKVAFP
                     VVTDDLTKLIERHVDDFDSYADLTATYGEGVEGVTEFRPGKRPVVRINEGLSGDQFRE
                     NRLRTTLTHELGHVLFHTWLFDERFGPSLFPPKQRRADDVQVCKRENLIDAPQVDWME
                     WQAGHACGAFLMPASRVRKIAREVAIRTPPPELTAVVPDSAFGRALILTLCEQFHVSR
                     DAAQVRSHRLGIFSEVDLGLAF"
     gene            1160..1372
                     /locus_tag="VN039_09095"
     CDS             1160..1372
                     /locus_tag="VN039_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46158.1"
                     /translation="MAKHHDKGLDGRMRDQDGEIRKKRSDTLVGTLRRTYGNTVAEGY
                     RSDAQLGTVLKREGLDSLDQLLKRRT"
     gene            1409..1873
                     /locus_tag="VN039_09100"
     CDS             1409..1873
                     /locus_tag="VN039_09100"
                     /inference="COORDINATES: protein motif:HMM:NF025808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiubiquitin domain-containing protein"
                     /protein_id="HWV46159.1"
                     /translation="MSHIDEIPPHMVRIHIDRQPYESPSPTTGAALYTLAAIAPHREL
                     FREAVGDQEDELVGRSDTSVQLQQDEHLYSQKEFVLIVNGQHKKWAEPKIAFEQVTHL
                     AFPDPPPPNVVITYTVEYEDGPRRNREGSLIAGKSVYVKNGMIFHVTETGRS"
     gene            1848..3041
                     /locus_tag="VN039_09105"
     CDS             1848..3041
                     /locus_tag="VN039_09105"
                     /inference="COORDINATES: protein motif:HMM:NF004804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HWV46160.1"
                     /translation="MSQKLVDRNPDLKRLRDDGYNIRIDGANLIVGSVPYVNAACEIK
                     RATLIFTLKLQQDRTTRPEPHWLSYVGEVPGEVPCNEHGIQLEIVIDVATHPSIAGMI
                     PSCRLSTKPKVGYYDDYYHQVTTYVKILEHQAQAIDPSVNARVYLPVRCDDDDDSPFL
                     YYDTASSRAGISDLSRKLAMPKVAIVGVGGTGSYVLDFVAKTLVREIHLFDDDEFKQH
                     NAFRCPGAVSFEELTPDLTKLEYYHQLYTRLRRGIVPHPYRIDPSNASDLVEMSFVFL
                     CLDSGEYKDAIMRTLEAANVPFIDVGMGIEHTGDELTGLLRVTTSTATMRSHIRENQR
                     VSFAKAQADNLYSRGIQVVELNALNAALAVIRWKKYCHFYHDFKGEHHSIYAVSTNKI
                     FNEDL"
     gene            3038..>3379
                     /locus_tag="VN039_09110"
     CDS             3038..>3379
                     /locus_tag="VN039_09110"
                     /inference="COORDINATES: protein motif:HMM:NF039808.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6527 family protein"
                     /protein_id="HWV46161.1"
                     /translation="MMTRQTALMPSFVDIIPEVLEEGVLYVSVRYKTACHKCSCGCGK
                     KVVTPLTPTGWRITYDGRTVSLYPSIGNWKLACQSHYWITNNRVRWAEEWTAQEITEG
                     FAHDTKLKENYY"
BASE COUNT          813 a          937 c          915 g          714 t
ORIGIN      
        1 gtatgaccac tgaaaccttt ggattcgtga tcgcctccaa gcggcgcgag aagggataca
       61 gtcaaaagga gctcgcggaa cgcatcaagc ttcctggcga cgcagggagc gccaagtcga
      121 tatcgccgca gtacctcaac gacatagagc acaacaagcg cagtccgtcc tctcccgaac
      181 tggttgcgca attttcgagg gtacttggct tgaacgctga ctacctagcg ttcctggcag
      241 accgttggcc cgaaagtctg agacggcaaa taaagtcaga atcggatttc gccagcttcg
      301 tcactgcttt tcgtcggacc cacggtactt gagactcatc cctgacccca ctggacgatt
      361 tactcaacgc ccgttctatg aagcggggga gctcgacgtc gagtgcgaaa aggtgatgtc
      421 ccagtttttg cgttcaatcc atgggaaggt tgcttttcca gtcgtaaccg atgacctgac
      481 caagctgatt gagcggcacg tggacgactt tgactcctac gccgacctca ctgctacgta
      541 cggtgaaggc gtggaaggtg ttacggagtt tcggccaggt aagcgcccag tggtgcgcat
      601 caacgaaggg ttgagcgggg atcagttccg agagaatcga ctgcgcacca cgttgacgca
      661 cgaacttggg cacgtgcttt ttcacacctg gctgttcgac gagcgctttg gcccctcgct
      721 atttcctcct aagcagcggc gtgcggatga cgttcaagtt tgtaagcgag agaatcttat
      781 cgacgcgcct caggtcgact ggatggaatg gcaggcaggc catgcatgcg gcgcattcct
      841 aatgccagcc tcacgagtgc ggaaaattgc acgtgaagta gctatacgca caccaccgcc
      901 ggaactcacc gcggttgttc cagactctgc cttcgggcgc gcacttatcc tgaccctctg
      961 cgagcaattc cacgtctccc gtgatgccgc acaagtgcga tctcatcgtc tcggtatctt
     1021 ttccgaggtc gatctcggtc tagcgttcta gaagctgctg tcagcaactt gcattccact
     1081 gcgggaggtg atagcgttta ccgcgaatcc gctaatctgc ggattcacgg actacgattc
     1141 cttccggatg aggtgcacga tggctaagca tcacgataag ggcttggatg gtcgcatgcg
     1201 cgatcaagac ggcgagatcc gcaagaagcg gagcgacacg ctcgtcggaa cactgaggcg
     1261 tacctacgga aatactgtgg ccgagggtta ccggtcagac gcgcagctcg gcacagtgct
     1321 gaagcgcgag ggcttggact ccctggatca attgctgaaa cgccggacct gacctttcca
     1381 tcaaccttca gcctaacgcg gaggatccat gtcacacatc gacgagatcc caccacatat
     1441 ggtgcgcatc cacatcgatc gccagcccta cgagtcgccg tcgccgacta cgggtgctgc
     1501 gctttacacc ttggcagcca tcgccccaca tcgcgaactt ttcagggagg cagtcgggga
     1561 ccaggaagat gaactcgtcg gacgtagtga cacatccgtc cagctgcagc aggacgaaca
     1621 tctctactca caaaaagaat tcgttctcat cgtgaacggc cagcacaaga agtgggcgga
     1681 gcccaagatt gcattcgagc aggtgacgca tctggcgttt ccggatcccc cgccgccgaa
     1741 tgtcgtcatc acctatacgg tcgagtacga agatggccca cgacggaaca gggaaggctc
     1801 gttgattgcc ggcaaatccg tctacgtcaa gaatgggatg atcttccatg tcacagaaac
     1861 tggtcgatcg taatcctgat ctcaagcggc tacgcgacga tggttacaac atccgcatcg
     1921 acggtgccaa cctgattgta ggtagcgttc cttacgtgaa cgcggcatgc gagattaagc
     1981 gcgcaacgct gattttcacg ttgaagctgc aacaggatcg taccacgcgc ccggagccac
     2041 actggcttag ctacgtaggg gaggtaccag gggaggtacc gtgcaacgag cacggtatcc
     2101 agcttgagat tgtcattgac gttgctaccc acccatcgat cgcaggcatg atcccaagct
     2161 gccggctttc tacgaagccc aaagttggtt actacgacga ctactaccac caggtcacca
     2221 cttacgtaaa gatccttgaa catcaggcac aagcgatcga tccttcggtc aacgcaaggg
     2281 tttatttgcc cgtccggtgc gacgacgacg acgattcacc ctttctttac tacgacacgg
     2341 catcaagccg tgcgggcatc agtgacctct caaggaaact agccatgccc aaagtagcca
     2401 tcgtaggcgt gggtggaaca ggtagctatg tgctcgattt cgtcgcgaaa acgttggtga
     2461 gggaaatcca cctcttcgac gatgatgagt tcaagcagca caacgcattt cgctgccctg
     2521 gcgcggtcag ctttgaggaa ctaacgcctg acctcacaaa actagagtac taccaccagc
     2581 tatacaccag gctgcgccgt ggaatcgtgc cgcaccccta ccggattgac cctagcaacg
     2641 ctagtgacct ggttgaaatg tcgttcgtat tcctttgcct ggacagcgga gagtacaaag
     2701 acgcgatcat gagaaccctg gaggccgcca acgttccctt tatcgatgtg ggaatgggca
     2761 tcgagcatac gggtgacgaa ttgacgggcc tactccgcgt cacgacttca acggccacca
     2821 tgcgtagtca tatccgggaa aaccaacggg tatcttttgc caaggcacaa gccgataacc
     2881 tgtattctcg gggcatccaa gtcgttgaac tcaatgcatt gaatgcagcg ctcgccgtta
     2941 tccggtggaa gaagtactgt cacttctatc atgacttcaa aggcgaacat cattcaattt
     3001 atgcggtttc gactaataag atttttaacg aggacctatg atgacaaggc aaacggccct
     3061 catgccgagt tttgtcgata tcattccgga ggtgttggag gaaggggtgc tctacgtatc
     3121 ggtgcgctac aagacggcct gccacaaatg cagctgcggg tgcggaaaga aggtagtaac
     3181 cccattaacg cccaccggat ggcgcataac ctacgacgga cgcacagtgt ctctgtaccc
     3241 ctcgataggc aactggaagt tagcgtgtca gtctcactat tggattacaa ataatcgagt
     3301 gaggtgggca gaagaatgga ccgctcaaga aattacagag gggtttgcgc acgacactaa
     3361 attgaaggaa aactactac
//
LOCUS       DATMTI010000574         8476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_657786, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000574 DATMTI010000000
VERSION     DATMTI010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657786"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VN039_09115"
     CDS             complement(<1..583)
                     /locus_tag="VN039_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HWV46162.1"
                     /translation="MKARALAILGTGSDVGKSLITAGICRLLRRTGVHVAPFKAQNMS
                     LNSFVTPGGHEIGRAQALQAEACGIPPHVDMNPILLKPESDNCSQVVVHGKVWGKREA
                     SAYFDGRQGLWSVVEESYARLAGQYEVIVIEGAGSAAEVNLREHDLVNWRVTELADAQ
                     VVLVADIDRGGVFAQVLGTLDLLLPDERARVCGV"
     gene            complement(580..1302)
                     /locus_tag="VN039_09120"
     CDS             complement(580..1302)
                     /locus_tag="VN039_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HWV46163.1"
                     /translation="MKGPATRVHTRHQVIGRTLVITLGGRRRILSSAPQGGGFRSASH
                     IINHQVDARPGMNGPLPDPARYLRKLASELSIGYHTVGLMTAVPMTQLVTARVSAGEL
                     WVECFATVGVTNAVCAGEWPPSVAHRKEIYSTRVGTINLILITNGSLSTSAMVGAVQV
                     ATETKTGVLRDHTIPSWKGRDLATGTGTDAVVVACRLRGEGPLHTYSGTHTMMGALVG
                     RAVSHCVSRGLAKARKWQEMHR"
     gene            complement(1305..2414)
                     /gene="cobD"
                     /locus_tag="VN039_09125"
     CDS             complement(1305..2414)
                     /gene="cobD"
                     /locus_tag="VN039_09125"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HWV46164.1"
                     /translation="MPRLKPRLHGGDIYGAARELNRDPAEILDFSASINPLGPSSQVW
                     KAITASRHLLSHYPDPHCWDLQQALATFWHADPEQIVVGNGSTELIDVLPRALGIHRL
                     LVVQPTFSEYAAAIERAGGQAMALYANRNDQYALPIDRLYRVMETGRIDGRSIDGIVI
                     CHPNSPTGQACSADDVARLASIAKRRGLWLVIDEAFADYCPERSVLPHASSWPQVVVL
                     RSMTKFYALPGLRVGYAVATRAVIRRLRRQLPPWSISVMGQIAALAALNDAAHARRSL
                     QFMARERERLKQMLAALPGCVVMPTWTNYFLVELPRGRHARDVTEQMRNEGLLIRDCS
                     SIPGCNARSIRLAVRSQQENDRLIQALSNLLHDGV"
     gene            complement(2402..3358)
                     /gene="cbiB"
                     /locus_tag="VN039_09130"
     CDS             complement(2402..3358)
                     /gene="cbiB"
                     /locus_tag="VN039_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HWV46165.1"
                     /translation="MTGGELLIASALDAMCGDPRWLPHPVRVMGRCITWLDHRVRMIC
                     RSRTSLRFAGICLAGGLPIMTYCIGAVMIEEAERFAAWLGSVLSIGLASTTLAARDLW
                     DHVRAVNGPLGVGDLPSARRAVAMIVGRDTAELSESGVARATVETVAESTADGLIAPL
                     FYLAIGGAPLALAYKAVNTLDSMIGHQDERYADFGWASARLDDLTNWIPARMAAVLLI
                     VGAGLTTGQLEQARNGWRVFRRDRSKHPSPNSGRPEAAMAGILGVKLGGTNSYDGIAQ
                     ERPVLGQEGRDAGPGDIMAAAKIMAAASVLGISLAVGFRCLA"
     gene            complement(3355..4119)
                     /locus_tag="VN039_09135"
     CDS             complement(3355..4119)
                     /locus_tag="VN039_09135"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HWV46166.1"
                     /translation="MGTVARPFIFAWHFLTTIPLSRTHHEPTAPELAASMAWYPIVGL
                     LIGGGLIVADFGLTAILAPAVVNVLLIALLVLLTRGLHQDGLADTLDGLAGGRIPAER
                     LSIMRDPRIGAIGATGLFLSLILRYAGLMVLPHELRLPAVLCMPAMGRWAMVTSAWIS
                     PYARTEGGLAAPFLTHLSWRHVALSTAVLTIALTVGFGAVGACLILLAGTLIVLVGWR
                     ACRRWFGGITGDTLGAANEVIEILFLLFVPLLLRLS"
     gene            complement(4125..5177)
                     /gene="cobT"
                     /locus_tag="VN039_09140"
     CDS             complement(4125..5177)
                     /gene="cobT"
                     /locus_tag="VN039_09140"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWV46167.1"
                     /translation="MPIQDLCRLIQPLNASLHGKAQARLDRLTKPLGSLGRLEELAAS
                     YVAITGELKPNVPRAVVFTFAADHGVALEGVSAYPREVTPQMVLNFLRGGAGVNVLAR
                     HAGVDVRVVDIGVDYEFGAVPGLLDRKVMKGTRNLAVESAMTRSQAEQAVMVGIELAT
                     EAVREGIGLIGTGEMGIGNTTPSAAITAVITGRPAEEVTGRGTGVDESGRMHKAAVIQ
                     RALELHCLNPTDPLDVLAKVGGLEIGGLAGLMLGAAAVRVPVVLDGFIAGAAALIAVG
                     LQPLCREYLIASHRSVEQGHRAVLDHLRLKPLLDLDLRLGEGTGACLGMGLVCAAIKI
                     YTEMATFDEAGVSDKA"
     gene            complement(5192..5752)
                     /gene="cobU"
                     /locus_tag="VN039_09145"
     CDS             complement(5192..5752)
                     /gene="cobU"
                     /locus_tag="VN039_09145"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HWV46168.1"
                     /translation="MSQRKSSKRRPARREKGRIILVLGGASSGKSEAALRLAGSRGPR
                     AFVATGQGLDDEMAARIARHQATRSTDWETVEEPLEVVAWFAKKGPRYRTILFDCVTL
                     WLSNLVGTGPKESVVLERVGILLHMMQIAAARVVIVSNELGFGLVPTEPSTRAFRDLA
                     GRVNQQIAAGADEVYLVVSGLPLRLK"
     gene            complement(5730..6326)
                     /locus_tag="VN039_09150"
     CDS             complement(5730..6326)
                     /locus_tag="VN039_09150"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWV46169.1"
                     /translation="MRITKVYTRTGDAGKTRLAGGQQVWKDSLRVEAYGAVDELNASV
                     GVVRVINADMNEESPAAQQLEEELRRVQNKLFDVGSILATAPGQTFKNMPQVVAQDVL
                     RLEKLMDRCQKDLEPLKEFILPGGGKVSGFLHQARTICRRAERVCIALSKMEPVDPTI
                     IKFLNRLSDALFVLGRWVAKTQGEPEFLWERDVTKKVE"
     gene            complement(6326..7042)
                     /gene="bluB"
                     /locus_tag="VN039_09155"
     CDS             complement(6326..7042)
                     /gene="bluB"
                     /locus_tag="VN039_09155"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HWV46170.1"
                     /translation="MNEGYNEVSSAMRIDSRFSNAERDAVYRAIFERRDVRRNFLPMP
                     IPEHILKRVLTAAHHAGSVGFMQPWDFVVVSDRSTKRAVKRMFQKANADAATRYRGKR
                     AALYRNLKLEGIEEAPINLGVTCSRQRGGPHVLGRSTVRDTDLYSTCCAIQNLWLAAR
                     AEGIGVGWVSILDHSALKRVLGVPKSVKVLAYLCLGYVSNFATRPDLETAGWRERIPL
                     EHLIHYESWGNTVTERKGKR"
     gene            complement(7230..7829)
                     /gene="cobO"
                     /locus_tag="VN039_09160"
     CDS             complement(7230..7829)
                     /gene="cobO"
                     /locus_tag="VN039_09160"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWV46171.1"
                     /translation="MTDQVEHKARMERLKASVDRRIEEAQQEKGLLIVHTGAGKGKST
                     AALGMVLRCIGHGWKVAVVQFIKGAIDTAEERALKSFGDRVIFLRMGEGYTWETQDGA
                     RDTQFAQQAWETACEFLRDASYAMVVLDEFNIALQYDYIRLEEVLSVLRDRPPMQHVV
                     ITGRGGPAALLDEADLVTEMKQVKHPFRKGVKAQPGVEF"
     gene            complement(7835..>8476)
                     /locus_tag="VN039_09165"
     CDS             complement(7835..>8476)
                     /locus_tag="VN039_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HWV46172.1"
                     /translation="GVAQDRAFCFYYPDNLDVLEAAGAEVVKFSPLSDQGLPNVEMLY
                     LGGGYPELHGEALARNVAMRAAVKRFAERGGVIYAECGGLMYLAEAIRDFDGRSHEMV
                     GIFPAKAVMQRHGLTLGYRRVECSRRCILGEVGVTARGHEFHYSTLVVREPLQYTCAL
                     SDADGHSKGQDGLTKHNVLALYTHLHFSSRPQIGTALVDAARQAAVRMSMSAG"
BASE COUNT         1844 a         2693 c         2195 g         1744 t
ORIGIN      
        1 tgaccccgca gactctggcg cgttcgtctg gtagcaataa atccaaggtt ccaagaactt
       61 gcgcaaaaac cccgccccga tcaatatcgg ccaccagtac gacctgagca tcagccaatt
      121 cggtaacccg ccaattgacc agatcatgct cacggaggtt gacttccgcc gcactgcctg
      181 ccccctcaat cacgatcact tcgtactggc cggccaaccg agcgtaactt tcttcgacca
      241 cagaccagag cccttgtcgt ccgtcgaagt acgccgatgc ctcgcgtttc ccccacacct
      301 tgccatggac cacaacctgc gaacaattat cggattccgg cttgaggaga atcgggttca
      361 tgtcaacatg cggcggaatc ccgcatgcct ctgcctgcag agcttgagct cttccgattt
      421 catgaccgcc aggagtgacg aacgaattca gcgacatgtt ttgagctttg aatggtgcca
      481 catggacgcc ggttcgacga agcaggcgac agattccggc tgtgatcaaa ctcttcccta
      541 catctgagcc ggtaccgaga atcgcaagcg ctcgcgcctt catcggtgca tttcctgcca
      601 ctttctcgcc ttggctaagc ctctggagac acaatgagag accgctcttc caaccagcgc
      661 acccatcatt gtgtgggtcc cgctgtaggt atgcagcggt ccctcaccgc gcaagcggca
      721 cgcaacgacc accgcatcgg tacccgtccc agtcgcaagg tctctccctt tccaactcgg
      781 tatagtgtgg tcacgcaaga cgcccgtctt tgtctcagtg gcaacctgca ccgcgccgac
      841 catggcggag gtcgagagac taccattcgt gataaggatg agattgatcg ttccgactct
      901 cgtcgaatat atttcctttc gatgagcgac actcggtggc cactccccgg cacaaaccgc
      961 attcgtgacc ccgaccgtgg cgaaacactc tacccagagc tctcccgccg aaactcgagc
     1021 ggtgacaagc tgtgtcatcg ggactgccgt catgagcccg acagtgtggt atccgatgct
     1081 gagttccgaa gccagctttc gcaagtaacg tgctgggtcg ggcaatggtc cattcatacc
     1141 ggggcgcgca tctacctgat gattgataat gtgtgaggcg gacctgaatc cacccccttg
     1201 cggtgcagat gaaagaatac gccttcgccc tccaagagtg atcacgagcg tgcgtccaat
     1261 cacttgatgc cgggtgtgga cgcgtgtagc aggtcccttc atcactacac accgtcgtga
     1321 agcagattcg acaaggcttg gatgagccgg tcattctctt gctgagatcg cacggcaagt
     1381 cggatcgaac gagcgttgca cccgggaatg gatgaacaat cgcggatcaa caacccctca
     1441 tttcgcatct gctcagtcac gtcacgcgcg tgccgaccgc gtggcaattc gacaaggaag
     1501 taatttgtcc atgtcggcat cacaacacag ccgggcagcg cggcaagcat ttgcttcaac
     1561 cgttcgcgct ctcgtgccat gaattgcaga cttcttcgcg catgtgccgc atcattcaaa
     1621 gcagcgagtg cagcgatctg tcccatgacg ctgatcgacc aaggtggcag ttgccgtcgc
     1681 aagcgtctga tgactgcacg ggtcgccacc gcatatccca cccgtagtcc cggcaaggcg
     1741 tagaatttcg tcatgctgcg taagacgacg acctgcggcc aggacgacgc gtgcggcagg
     1801 acagatcgtt ctggacaata gtcggcaaat gcttcatcga tcaccagcca aagacctcgc
     1861 cgcttggcga tggacgctaa ccgcgcgaca tcatcggctg agcaggcttg cccggtcgga
     1921 ctattgggat gacagatgac gattccatca atggagcggc cgtcgatccg tcctgtttcc
     1981 atcactcggt agagacggtc aatcgggagc gcatactggt cgtttcggtt tgcatagagc
     2041 gccatggctt gtcctccggc tcgctctatc gcagcagcat attcagaaaa ggtaggctgg
     2101 acgacgagga gccgatggat ccccaatgca cgaggaagca cgtcaatcag ttctgtggaa
     2161 ccgttaccga ccacgatctg ttccggatca gcatgccaaa atgtggccag cgcctgctgc
     2221 agatcccaac agtgaggatc cggatagtgg ctcagcagat gtctggacgc ggtaatggct
     2281 ttccagactt gagaagaagg ccccaacggg ttgatgctgg cgctgaaatc aagaatctct
     2341 gcgggatcac ggttcagttc tcgtgcggca ccatagatat ccccgccgtg caagcgcggc
     2401 ttcaggcgag gcatctgaaa cccaccgcta gagagatacc gagcacagag gctgccgcca
     2461 tgatcttcgc tgccgccatg atatcgcctg gtcctgcatc gcgtccttct tgaccgagaa
     2521 caggacgttc ttgtgcgatg ccgtcatagg agttggtgcc tcccaatttc actcctaaga
     2581 tccctgccat ggccgcttct ggtctcccgc tgttgggact aggatgtttg ctcctgtcgc
     2641 gacggaacac ccgccatcca ttgcgggctt gttccagttg tcctgtggtc aatcccgcgc
     2701 caacgatgag caagactgca gccatacgag ccggaatcca gttggtcaga tcgtcgagtc
     2761 gggccgatgc ccatccaaaa tccgcatagc gctcgtcttg gtgaccgatc atcgaatcca
     2821 gggtattgac ggccttatag gccaaggcta aaggagcgcc gccgatggca agataaaaca
     2881 gcggggcgat caggccgtct gccgtgcttt ccgcgaccgt ttccacagtc gcgcgggcca
     2941 ccccggattc ggaaagctcc gccgtgtctc gtccgacaat catggcgaca gctcggcgtg
     3001 cggatgggag atccccgacg ccgagcggac cattcacggc acggacatga tcccacagat
     3061 cccgggcagc aagcgtcgtc gaagccaatc cgatcgacag gaccgacccc agccatgctg
     3121 cgaaacgttc ggcctcttca atcatgacag cgccgataca atacgtcata atcggcaatc
     3181 ctccggccag acagattccc gcgaagcgaa ggctcgtcct actccggcag atcattcgta
     3241 ctcggtgatc gagccatgta atgcaccgcc ccatcacacg gactggatgc ggcagccagc
     3301 gaggatcgcc gcacatcgca tcaagagcag aagcaatgag gagttcgccg ccggtcatga
     3361 taaccggagc aagagaggaa cgaacagcag gaagagaatc tcgatcactt cattggcagc
     3421 cccaagcgta tctccggtga tcccgccgaa ccagcgacgg caggctctcc agccgacaag
     3481 gacgatcaga gtcccggcca ggaggatcag acatgcaccg accgctccga atcctaccgt
     3541 cagtgcgatc gtcaagactg ccgtggacag ggcgacgtgc cgccacgaca gatgggtgag
     3601 gaacggcgcc gccaatcctc cttctgttct ggcatagggc gaaatccaag ccgacgtgac
     3661 catcgcccaa cgtcccattg ccggcatgca gagcacggcg ggcaaccgca actcatgcgg
     3721 cagcaccatc aacccggcgt accggagaat cagcgagagg aagaggcctg ttgcgccgat
     3781 cgctccgatg cgcggatcgc gcatgatcga aagccgttcc gccggtatac ggccacccgc
     3841 caacccatcc aatgtgtcgg ccaacccgtc ctgatgaagc ccacgtgtca ataacaccaa
     3901 cagcgcgatc aaaagcacat tcacgacggc tggcgccaat atcgcggtca gtccaaagtc
     3961 ggccacgatg agcccgccgc cgatcagcaa accgacgatc ggataccatg ccatcgaggc
     4021 ggccagctcc ggtgcggtgg gttcatgatg cgttcggctt aaagggatcg tcgttaaaaa
     4081 atgccaggca aagataaatg gacgagccac ggttcccata cctgttacgc cttgtcggac
     4141 acgcctgctt cgtcgaacgt tgccatttcc gtgtaaatct tgattgcggc acaaaccaga
     4201 cccattccca agcaggcacc cgtcccttcg ccaagacgca agtcgagatc caacagcggt
     4261 tttagtctca gatggtccaa gaccgcgcga tgcccctgct caaccgaacg atgggaggca
     4321 atgagatact cacgacacaa gggctgaagc ccgacggcaa tcagcgccgc ggctccggca
     4381 atgaagccgt ccaacacgac aggcacccga accgctgctg cgccgagcat caggccggcc
     4441 aatccaccga tttccagtcc cccgactttc gccaagacat cgagtgggtc tgtcggattg
     4501 agacaatgta gttccagcgc tcgctgtatc accgccgcct tgtgcatacg gccggactcg
     4561 tcgacgccgg tcccccgtcc agtcacctct tccgcaggcc gaccggttat caccgccgta
     4621 atggcggcgc ttggtgtcgt attaccgata cccatctcac cggtgccgat caggccgata
     4681 ccttcccgca cggcttctgt agccagttca attccgacca tgacggcctg ctctgcttgg
     4741 ctgcgtgtca tcgccgattc aaccgccaga ttgcgtgtac ctttcatgac ttttcgatcg
     4801 agaagaccgg gcacggcgcc gaactcgtaa tcgacgccga tatccaccac tcgcacatcc
     4861 acaccggcgt gccgtgccaa cacattcact cctgctccac cccgtagaaa attcagcacc
     4921 atttgaggcg tgacttcccg aggataagcg ctcacccctt ccagagccac tccatgatcg
     4981 gcggcaaagg taaatacgac agcgcgtgga acattcggct tcagttcgcc ggtgatagcg
     5041 acgtaagacg ccgccaattc ttcgagacgc ccgagactac cgagcggctt ggtcagtcga
     5101 tccaatcgcg cttgcgcttt cccgtgcaga ctcgcattga gaggttgaat cagacggcac
     5161 aggtcctgta tcggcatgac ggctcctcta ttcatttcag gcgaagcggt aatccgctga
     5221 caactaggta tacttcatct gctccggcgg cgatctgctg atttacccgt ccggcaagat
     5281 cacgaaatgc tcgtgtcgac ggctcagtcg gaaccagacc gaatcctaac tcattgctga
     5341 cgatgaccac cctggccgcc gcaatctgca tcatgtgcaa aagtatgcca acccgctcga
     5401 gaaccaccga ttcttttgga ccggttccga ccagattact caaccacaat gtgacgcaat
     5461 cgaagagaat cgtccgatac cgaggaccct tcttggcaaa ccatgccacc acttcgagcg
     5521 gctcttcaac ggtctcccaa tccgtcgaac gtgtagcctg gtgccgagcg attcgtgccg
     5581 ccatttcatc atccaacccc tgccctgtgg cgacaaatgc gcgcggccct cgtgagccgg
     5641 ctaaccggag agcggcctcg ctttttcccg aggatgcccc accaagcaca agaataatgc
     5701 ggcctttttc tcgtcgcgcc ggtcgacgtt tactcgactt tctttgtgac atcgcgttcc
     5761 cataagaatt ccggctcgcc ctgcgtcttg gctacccagc gacccaagac gaacagcgca
     5821 tcgctcagcc ggttgaggaa cttgatgatc gtcggatcga ccggctccat ttttgacaga
     5881 gcgatgcata cccgttcggc ccgacgacag atcgtcctgg cttgatggag aaaaccggat
     5941 acttttccgc cacccggcag gatgaactct ttgagcggtt ccaaatcttt ttgacaacga
     6001 tccatcagtt tttccaggcg caacacatcc tgtgccacca cctgcggcat attcttgaat
     6061 gtctgccccg gcgcagtcgc gagaatgctg ccgacatcga acaacttgtt ctgtacccga
     6121 cgcaactcct cttcgagttg ttgtgccgcc ggggattcct cgttcatgtc ggcattgatg
     6181 acccgcacca caccgaccga tgcattgagc tcatcgacgg caccataggc ttcgacgcgc
     6241 aaactgtctt tccatacttg ctgtccaccg gccaatcgtg ttttccctgc atcgccggtt
     6301 ctggtataga cctttgtaat ccgcatcacc gtttcccctt gcgctcggtc accgtattgc
     6361 cccatgactc atagtgaatg agatgttcca gcggaatccg ttcacgccat ccggcagttt
     6421 ccaaatcggg tcgcgtcgca aagttcgata cataccccag acaaagatag gccagtactt
     6481 tgaccgactt gggcactccc aacacccgtt tcaaggcact gtgatccagg atgctgaccc
     6541 atcccactcc gatgccttcc gcacgtgcgg ccagccagag gttttgaatg gcgcaacagg
     6601 tgctatagag atccgtgtca cgcaccgtgg agcggcccag cacatgagga cctccccgct
     6661 ggcggctgca ggtcacgccg agattgatcg gagcttcctc gatcccttca agcttgagat
     6721 ttcggtagag cgcagcgcgt ttcccccggt aacgggttgc ggcgtcggca ttggcttttt
     6781 ggaacattcg tttcaccgcg cgtttggtcg accgatcact gaccaccaca aaatcccatg
     6841 gctgcatgaa tccaacggac ccggcatgat gagccgcggt cagcacacgt ttcaaaatat
     6901 gctccgggat cggcatcggc agaaagttgc ggcggacatc acggcgctcg aagatcgccc
     6961 gatagacggc atcgcgctcc gcatttgaaa atcggctgtc aatccgcatg gctgatgaca
     7021 cttcgttata cccctcgttc atgatggacg aatccccagc tcaccggtca cgatttttct
     7081 caagcagacg ggacagagac aatcctcata tcgtgctgcg atccaatcca tttgttcttc
     7141 tgtcacgccg atcgttccgc accaacaccg gtatccgccg cattgaaacg cctgtccgca
     7201 gtgttcgcag gttttctgga ccggtcctct caaaattcaa ctcccggctg cgccttgacg
     7261 ccttttcgga acggatgctt cacttgtttc atctccgtca ccagatccgc ctcatccaga
     7321 agtgccgccg gtcctcctct accggtgatc accacatgtt gcatcggtgg acgatcgcga
     7381 agcaccgaca gtacctcttc aaggcggata tagtcatact gcagcgcgat attgaactcg
     7441 tcgaggacga ccatggcata ggacgcatcg cgcaggaatt cacaagccgt ttcccatgcc
     7501 tgttgtgcaa attgcgtgtc tcgtgcccca tcttgagttt cccaggtata gccttctccc
     7561 atccggagga agatgactcg atcgccaaaa gattttagag cgcgctcttc cgcagtgtcg
     7621 atggcgcctt tgatgaattg cacgaccgct accttccagc catgcccgat acagcgcagc
     7681 accatcccca gcgcggcggt cgacttccct ttaccggcgc cggtatggac gatcaagagg
     7741 cccttctcct gttgcgcttc ctcgatgcgc cgatcaaccg acgccttaag gcgttccatt
     7801 cttgctttat gttcgacttg atccgtcatg gtgatcaacc cgcactcatg gacatccgaa
     7861 cggcggcttg acgggcggca tccaccagcg ctgtcccgat ctggggccgg ctggaaaaat
     7921 gcagatgagt atagagggcc agcacgttgt gttttgtcaa tccgtcctgc ccctttgagt
     7981 gtccgtctgc atcactcagt gcacaggtgt actgaagcgg ctcccgcaca accagcgtgg
     8041 aataatggaa ctcatggcct cgtgccgtga cgccgacttc accaaggatg cagcgtcgcg
     8101 agcattccac ccttctatac cccaaagtga gaccgtgtct ctgcatgacc gctttggccg
     8161 gaaagatgcc gaccatttca tgcgaccgac catcgaaatc acggatcgct tccgcgagat
     8221 acatcaaccc gccgcattct gcataaatca caccgcctcg ctcggcaaac cgcttgactg
     8281 cggctcgcat ggctacgttc ctggccagcg cctcgccatg caattccgga tatccgccgc
     8341 caagatacag catctcgacg ttcggcaatc cttggtcgct gagcggagaa aacttcacga
     8401 cttcagctcc ggccgcttcc agcacgtcaa gattgtccgg atagtaaaaa caaaaggccc
     8461 gatcttgcgc gacacc
//
LOCUS       DATMTI010000575         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_21351, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000575 DATMTI010000000
VERSION     DATMTI010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21351"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VN039_09170"
     CDS             complement(<1..310)
                     /locus_tag="VN039_09170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV46173.1"
                     /translation="MTDPVIRNMDPDDRESVVQLLGESDPWRKLGYSKDDWNRIFCPT
                     PRGRDCYVALLDGQVAGVAIVKQKFLLGDYLELLGVAVWARQKGIGGQLLRYIEELVF
                     K"
     gene            complement(303..1022)
                     /locus_tag="VN039_09175"
     CDS             complement(303..1022)
                     /locus_tag="VN039_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWV46174.1"
                     /translation="MQRRVLLRLLLGMLSAATPGLFVPVVGVAQVIKSGPPTCPGVAL
                     TFDLCPVRKGSGYDQPLMDYLITHRIPATFFMSGKWMAKHEADVDRLLGLGFFEVGTH
                     GEVHAHLPMHDADEQRTEILGPVKLLRKHYAHEATLFRPPFGEYNDVTVAVVNMLGLR
                     FIQWNIESGDPDPTLSAEQILSGVAARAKPGSIIVFHANGKGKQTRQVIEPLTSDILP
                     RKGLQPMTVSELLNCTPRAHD"
     gene            1092..2252
                     /locus_tag="VN039_09180"
     CDS             1092..2252
                     /locus_tag="VN039_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWV46175.1"
                     /translation="MRVPSWEIGRALGIPIRIHASWFLVFLLVTWTLSTGYLPESLPG
                     LTPERYWAMGGLAAVLLFLSVLLHELGHSYVALYYRIPIEKITLFIFGGVAHMRKEAP
                     TPLAEFLIALAGPAVSFVLGGLCFVFVELAEAIQRQHGLQGWIMLGALLGFVNMQLGL
                     FNMIPGFPLDGGRVLRAGLWAWGNDFHRATKQAAVVGLAFGLLLGLAGLVVMYGAVSG
                     GLPASMASNGGWILVIGMFLFAAALASRRQAAIRQSLATVPIRDLMVTTVTSIPSHCT
                     LDEAVNQYFQSYGYGGFPVLEDGRLVGLITVLEIQNVSPSLWLWRRVEQVMRPRSESL
                     VIEPDIPVIHAMERMAREGWDRLVVMQDEDIIGLVTHSAIVHFLQLRSSSSR"
     gene            complement(2294..>3045)
                     /locus_tag="VN039_09185"
     CDS             complement(2294..>3045)
                     /locus_tag="VN039_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HWV46176.1"
                     /translation="LFPLAFLVLYGVSFFVSQDVIGEQFMLSFLREFLPSLGERLAEE
                     LHRISVLESVRWLVFLSFFWFGGLVFYELDYTLNVVFESMPQRHPLISTAISIALVGS
                     TGLLLLMAYVGTQTIAFLTTYAPKLWGLDLVALAAHDFHLTYTLPFTLAFLAVSLLYR
                     LVPRRRPRWRAAMAGALIFSVLWVSAKLLFVSYSGYATVYTRLYGSLLEVVLLLLWVY
                     YSAGLLLFGGIIAHKLQQLACSSPTVSPDDA"
BASE COUNT          650 a          840 c          897 g          658 t
ORIGIN      
        1 tcttaaacac cagctcctcg atgtatcgca acagctgacc gccgattcct ttctggcgag
       61 cccataccgc cacacccaaa agctccagat agtcgcccaa gagaaacttc tgcttcacga
      121 tggcgacgcc tgccacctgc ccatcgagca gcgcgacgta acagtctcgt ccacgcggag
      181 tcgggcaaaa gatacggttc caatcgtcct ttgagtaccc caattttctc cagggatcgg
      241 attcaccgag cagttggacg acggactcac gatcgtccgg atccatgttc ctgataaccg
      301 ggtcagtcat gggctcgggg agtacagttc aacagctcgc tgacggtcat gggttggagt
      361 cctttccggg gcaggatgtc cgatgtgagc ggttcaatca cctgccgggt ttgctttcct
      421 ttgccgttcg cgtggaagac gatgatgctc cccggcttgg cacgcgcggc gactccggag
      481 agaatttgct cggcggagag cgtggggtcc ggatcacccg attcgatatt ccattggatg
      541 aatcgcaagc cgagcatgtt caccacggcg acggtcacgt cgttgtattc tccaaacggt
      601 ggccgaaaca acgtcgcctc gtgggcatag tgtttccgca acagtttgac gggtccgagg
      661 atctccgtgc gttgttcgtc ggcatcgtgc atgggaaggt gggcatgcac ctcaccgtgc
      721 gtacctactt cgaaaaaccc caggcctaac aggcggtcca cgtccgcttc atgttttgcc
      781 atccacttgc cggacatgaa gaatgtggcg gggatacgat gcgtgatcag atagtccatc
      841 aacggctgat cgtagccgga ccccttccgc acgggacaga gatcgaacgt caaggccacc
      901 cccgggcacg ttggtgggcc ggacttgatg acttgggcaa caccgaccac tggaacgaat
      961 aacccgggag ttgctgctga caacatgccg agcagcaggc gtagcagaac gcgtcgttgc
     1021 atacgggtca gtatacctga acggcggtga agaattgacc gtttacgtat tcggctgtta
     1081 cggtatggcc tatgcgcgta ccttcttggg aaatcggcag agcgttggga attccgatcc
     1141 gtatccatgc ctcatggttt ctggtctttc tcctggtcac ctggaccctc tcgaccggat
     1201 atctgccgga aagtttgccc gggctcactc ccgagcgtta ttgggcaatg ggtggcctcg
     1261 ccgcggtact gttattcctc tccgtgctcc tgcatgagct cggccattcc tacgtcgcat
     1321 tgtactatcg catcccgatt gaaaagatca cgttgtttat tttcggcggt gtggctcaca
     1381 tgcggaaaga agctccgaca cctcttgcgg aattccttat tgccttggcc gggccggcgg
     1441 tcagttttgt gctcggcggc ctctgttttg tctttgtgga actggccgag gcgattcaac
     1501 ggcagcatgg cctccagggg tggatcatgc tcggagcctt gctgggattc gtcaatatgc
     1561 aactcgggct tttcaatatg attccgggtt ttccgctgga cggcggacgg gtgttacggg
     1621 cgggattgtg ggcatgggga aacgattttc atcgggctac caaacaagcc gcagtggtcg
     1681 gcctggcgtt tggattgctg ctcggactgg ccggccttgt ggttatgtac ggagccgtga
     1741 gcggcgggct gcctgcttcg atggcatcga acggcggatg gattctcgtc atcggcatgt
     1801 ttctcttcgc cgcggctctg gcgagtcgcc gtcaagccgc catacgtcag tcactggcga
     1861 cggtccctat tcgggattta atggtgacga cggtcacctc cattccatcg cattgcacgc
     1921 tggatgaggc ggtgaaccaa tacttccagt cctatggcta tggcggcttt cctgtgttgg
     1981 aggatgggcg actggtggga ctcatcaccg ttttggagat tcagaacgtg tcgccctcgc
     2041 tatggctctg gcgccgagtc gaacaggtga tgcgcccacg ttcagaatcg ttggtgattg
     2101 agccggacat tcctgtgatc catgcgatgg aacgcatggc ccgtgaagga tgggatcgat
     2161 tagtcgtcat gcaggacgag gacattatcg ggctcgtgac ccattcagcc atcgtgcatt
     2221 ttttgcagtt acgtagctct tccagccgct gagcggcggc actcgccgtc tgtcatccgc
     2281 ttcaacatct ttgctaagca tcatcaggcg acaccgtagg cgacgagcag gcgagctgtt
     2341 gcagcttgtg ggcgatgatg ccgccgaaca gcaagagccc ggcggaataa tagacccaca
     2401 ggagcaagag gacgacttcc agcaacgatc catagagccg agtatagaca gtggcatagc
     2461 cgctatagct gacgaacaac aatttggccg agacccagag aacactgaag atcagggctc
     2521 cggccattgc ggctcgccag cgcgggcggc ggcgaggaac aaggcggtac aataggctga
     2581 ccgccaggaa tgcgagggta aaaggcagag tgtaggtcag atgaaaatca tgcgcggcta
     2641 gggcgacaag atcgagcccc cagagcttgg gcgcataggt ggttaaaaag gcgatggttt
     2701 gcgtgccgac atacgccatg agcagcagca gcccggtcga tccaaccagc gcgatggaga
     2761 ttgccgttga aatcagcgga tgacgctgag gcatgctctc aaaaaccacg ttgagcgtgt
     2821 agtccaactc ataaaagaca agcccgccga accagaaaaa cgacaaaaat acgagccatc
     2881 gcacgctctc caacacactg atccgatgga gctcttccgc cagtcgctcg cccaacgacg
     2941 gtaaaaattc tctgaggaag ctcagcatga actgttcgcc gatgacatcc tgactgacga
     3001 aaaaactcac tccatacagg accagaaacg cgaggggaaa gaggg
//
LOCUS       DATMTI010000576         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_785521, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000576 DATMTI010000000
VERSION     DATMTI010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785521"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="carB"
                     /locus_tag="VN039_09190"
     CDS             complement(<1..815)
                     /gene="carB"
                     /locus_tag="VN039_09190"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HWV46177.1"
                     /translation="MPKRTDIQSILMIGSGPIVIGQACEFDYSGTQACKALKAEGYKV
                     ILINSNPATIMTDPEMADRTYVEPITLDVLEKVIDRERPDALLPTMGGQTALNTTMGL
                     VKRGVLEKYGVSLIGASAEAIHKAEDREAFKQAMHRIGLRVPKSGTAHTRQDALTILD
                     TVGFPAIIRPSFTMGGTGGNIAYNREEFERLIDWALAMSPVTQVLIEESVIGWKEYEL
                     EVMRDLKDNVVIVCPIENFDPMGVHTGDSITVAPAMTLTDKEYQRMRDAALRI"
     gene            complement(834..1796)
                     /locus_tag="VN039_09195"
     CDS             complement(834..1796)
                     /locus_tag="VN039_09195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWV46178.1"
                     /translation="MAQSADQEINRLFSTPIDRRALLLRGARRMLGLAWLLSFGATGG
                     LVATLEGCVRAPGTARDQFIYISEEKEIAMGISAYHEVLRKAPISDDPELNELVNRVG
                     KRIAAVANKPEYQWEFAIIRDDRMINAFALPGGKVAVFTGILKLTKNEDGLATVIGHE
                     VAHALQRHGAERYSRSILETIGQVGALAAGAAVGRPDAAMAAMSAYGVGVSLPFGRKQ
                     ESEADYIGLKLMAQAGYDPREAVPFWERMSGCPRQMIDKVCFRSQHNIPEFLSTHPSD
                     LSRINQIEAWIPEALKYYHAAQGGEPPAPAPYHPLIGPPDLPSS"
     gene            complement(1877..>2296)
                     /locus_tag="VN039_09200"
     CDS             complement(1877..>2296)
                     /locus_tag="VN039_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HWV46179.1"
                     /translation="PRKDMGSPFRIMTTEERAIFQGLIDSFYQRFLNIVQEGRSNLQM
                     EQIKRLADGRIYTGEQAKAAGLVDEIGYLEDAIALAKKKAGLTEAKVVTYQRPGEYSN
                     NVYSKLLTPSGFAGLADLDLMSFVRGGTPQFMYLWMP"
BASE COUNT          494 a          694 c          580 g          528 t
ORIGIN      
        1 atgatacgaa gtgccgcgtc ccgcattcgc tggtattctt tgtcggtcaa cgtcatagcc
       61 ggcgcgaccg tgatactgtc tcctgtgtgt acgcccatgg gatcgaagtt ttcaatcgga
      121 cacacgatga cgacgttgtc cttcagatcc cgcatgacct ccaattcgta ctctttccac
      181 ccaatgaccg attcttcgat caacacctga gtgaccggac tcatggccag agcccaatcg
      241 atcagcctct cgaattcttc acggttatag gcgatgttcc cgccggttcc acccatcgtg
      301 aaggatggac gaatgatcgc ggggaagccg acggtatcga gaatggtcag tgcgtcctgt
      361 cgcgtatgcg ccgtaccgct cttcggcacc cgcaggccga ttcgatgcat cgcttgcttg
      421 aacgcttcac ggtcctccgc tttatgaatg gcttccgctg aagcgccgat gagcgaaaca
      481 ccgtattttt cgaggactcc tcgcttgacc aaccccatag tggtattgag ggccgtttgc
      541 ccacccatgg tcggaagcaa ggcatccggg cgctcccgat cgatcacctt ctccagcaca
      601 tctaaggtaa tcggttcgac atatgttcga tcggccatct ccggatccgt catgatcgtg
      661 gccggattgc tgttgatgag gatgacttta taaccctccg ctttcagcgc tttgcaggcc
      721 tgcgtgccgg aataatcgaa ctcacaggct tggccgatga cgatcggtcc agaaccgatc
      781 atgagaattg actgaatgtc cgtccgttta ggcatggtgt cttaatagtc ggattaactg
      841 gatgggagat cgggcggccc aatcagcgga tgatacggag ccggcgcagg cggctcacca
      901 ccctgcgccg catggtagta cttcaaggcc tcaggtatcc aggcttcaat ctggttgata
      961 cgcgacagat cggaaggatg ggtggagaga aactccggaa tattatgttg cgaccgaaaa
     1021 caaaccttgt cgatcatctg cctaggacag ccgctcatcc gttcccaaaa cggaaccgct
     1081 tcccggggat catagccggc ctgcgccatc agtttgagcc caatgtagtc ggcttcagat
     1141 tcctgctttc tcccaaacgg caaggagacc ccgaccccat aggcgctcat cgcagccatc
     1201 gccgcatccg ggcgtccgac agcagctcct gccgccagtg ccccgacttg gccgatcgtt
     1261 tcaaggatgc ttcggctata ccgctccgcc ccatggcgtt ggagcgcatg cgccacctca
     1321 tgcccgatca cggtcgccaa accgtcttcg ttcttcgtaa gcttcaggat cccagtgaag
     1381 acggccacct tgccgcccgg caatgcgaag gcattgatca tgcggtcatc tcttatgatg
     1441 gcaaactccc actgatactc aggtttgttg gcaaccgccg caattcgctt cccgacccgg
     1501 ttgaccaact catttaactc aggatcgtca ctgatcggag ctttgcgcag cacctcatga
     1561 taggcgctga tgcccatcgc aatttccttc tcctcggata tatagatgaa ttgatctcgc
     1621 gccgttcccg gggcgcggac gcatccctct agagttgcga ccaaaccccc agttgcccca
     1681 aaactcagaa gccaagccaa cccaagcatc cttcgagccc ctcgcagaag caaggcgcga
     1741 cggtcgatcg gtgttgagaa cagtctgttg atctcctgat ctgccgactg agccatcctg
     1801 aatccttagg tagaagccga tcgtcactat attaccagtc atgatgccac tagtcatgcg
     1861 atcggcacct ccctcattat ggcatccaca agtacatgaa ctgcggagtt cccccacgga
     1921 caaacgacat gaggtcgaga tcggcaagac cggcgaaccc gctcggtgtc aacagcttgg
     1981 aatacacgtt gttggagtat tcaccaggac gttggtacgt cacgacttta gcctctgtca
     2041 gaccggcttt tttcttggcc aatgcgattg cgtcttcaag atagccgatc tcatcgacca
     2101 aaccggccgc ttttgcttgc tcgccggtat agattcgtcc atcagccaac cgtttgatct
     2161 gctccatctg taagttggag cggccctcct gcacgatgtt caagaagcgc tgataaaacg
     2221 aatcaatcag tccttgaaag atggcccgtt cttcggtcgt catgatccga aacggcgagc
     2281 ccatatcctt gcgagg
//
LOCUS       DATMTI010000577         3370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_658081, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000577 DATMTI010000000
VERSION     DATMTI010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658081"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..304
                     /locus_tag="VN039_09205"
     CDS             179..304
                     /locus_tag="VN039_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46180.1"
                     /translation="MELLVEDLEIVERKIPNPWPGCRPLAEMVYAPIKNHAGDDG"
     gene            459..776
                     /locus_tag="VN039_09210"
     CDS             459..776
                     /locus_tag="VN039_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46181.1"
                     /translation="MNAKLLHIALVATTALSAAAPGCSQTAAERRAASPTLGERVKQD
                     SVTGRVHDIGAKYVSIRESNGETTRVRVDDHTKMDRVAVGDQVKAYVSEDGYASTIQR
                     VSD"
     gene            complement(805..1662)
                     /locus_tag="VN039_09215"
     CDS             complement(805..1662)
                     /locus_tag="VN039_09215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWV46182.1"
                     /translation="MTDSANKEADHGDEELNEASLVVNTSEVGPSRREILLHGSKVMA
                     SIGVAALFGNPGTFSRESYVMDRSAMSLLDSLTPVRTALLVIDMQRDFLLPEGYAAQA
                     GLDIAPLVATIRPIEKLLAVGRKAGLLIVHTREGHAPDLSDCPPYKLERSRRAGAEIG
                     SKGPLGRLLVRGEVGHDFVEALRPLEQEIVIDKPGYSAFTHTGLQQALAKRGIETLIL
                     TGVTTEVCVSSTLRGAIDLGYRCITVSNACASSDLGLHKASLAMIGVEGGIFGEVATT
                     AEVIKRLAP"
     gene            1855..3243
                     /locus_tag="VN039_09220"
     CDS             1855..3243
                     /locus_tag="VN039_09220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV46183.1"
                     /translation="MSMDSIHEVVEPVVDPEFGPWLAQASILVVDDEPGMRNFLLKTL
                     GSRCRSIQEASDTKEASRKLDAQHFDVVILDNVMPGENGLDWLAKQRAIGLFSDVILM
                     TAYADLDTAIEALRAGVVDFVLKPFRSNQLLNAVARCLDRTRLQRENYVLRHELKSLS
                     HQVFLRSKLLGDSVVIQQAREAIARVAPLPTSVLFTGQSGTGKEVAARSLHSLSNRAD
                     QVFVPINCGAISPDVIESELFGHLKGAFTGASRPRDGLFMHAQGGTLFLDEIGELPYM
                     LQSKLLRVLEDRRVRPVGSEREIPFDARFVFATNTDLKGRVKAGTFRSDLYFRINVMQ
                     IHLPPLRDRGDDVQELAALFMREIAQQLGMPPVTIDERIREALARYDWPGNIRELRNL
                     IERTVILGAFPEDFERFVHDDDTGGQSLAEVEQRHIVSVLREAGGDREEAAQRLGISR
                     KTIDRKCASWRI"
BASE COUNT          712 a          971 c          974 g          713 t
ORIGIN      
        1 cgtggccttg aactggaacc tggatgtgct cttctcgaag gatctggctc actttcgaac
       61 gaagcgctcc cgtatcccac atgcggcagc aaaccggcag agacctaccg aatccttcac
      121 gccactcttc atcaaccgcc ccccagactt cggattacac aagcaggagt aaggagacat
      181 ggagcttctc gtggaagatt tggagatcgt cgaacgtaag attccgaatc catggcccgg
      241 ctgccggcca ttggcggaga tggtgtatgc gccgatcaaa aaccacgcag gtgatgatgg
      301 atgaactgag ccaaggcgag aaagctgaca ttgcgcgtgc ttctgcattg gtttgttata
      361 gaggacgatg cagcggtctg caactagggt cactcccagc acggaatcat cgcacctcta
      421 aggtagaatt aagtaagcca attgaaggag gtgctattat gaatgccaaa ctgctgcaca
      481 tcgccctcgt ggctacaacg gcattgtctg ccgcggcgcc gggatgcagc cagacggcgg
      541 ccgaacgacg tgcagcctca ccgactttgg gagaacgggt caaacaggat tctgtcaccg
      601 ggagagttca cgatatcgga gcgaagtatg tgtccatcag ggagagcaac ggtgaaacaa
      661 cacgcgtccg cgtcgacgat cacaccaaga tggaccgcgt ggcggtgggg gatcaggtca
      721 aagcctatgt ttcggaagac ggctatgcct cgacgattca gcgagtctcc gactaactat
      781 cggccgaaga ccaaccgatc ggtatcatgg agcgagccgc ttaatgacct cagcggttgt
      841 ggcgacttcg ccgaagatgc cgccttccac gccgatcatg gccaacgaag ctttatgcaa
      901 accgagatcg ctcgaagcgc aggcattact cacggtaatg cagcggtagc cgaggtcgat
      961 tgcaccacgt aacgtggagc tgacgcacac ctctgtggtg acgcccgtca aaatgagtgt
     1021 ttcaatgccg cgcttcgcca gcgcttgttg caatccggtg tgagtgaacg cactatagcc
     1081 gggcttgtcg ataacgatct cctgctccag gggacgtaaa gcctccacga aatcgtgccc
     1141 gacttcaccc cgcaccagca gacgaccgag cgggcctttc gagccgattt ccgcaccggc
     1201 ccttcgactc cgctccagtt tgtatggcgg acaatcggac aggtccggcg catggccttc
     1261 gcgggtatgc acgatcagta gtccggcttt tcgccccact gcaagcagct tttcgatcgg
     1321 acggatggtc gcgaccagcg gcgcgatgtc gagtcctgcc tgcgccgcgt aaccctccgg
     1381 caggagaaag tcacgctgca tgtcgatcac caggagagcc gtgcgaacgg gcgtcagtga
     1441 atcgaggaga ctcattgctg atcggtccat tacatatgat tctcttgaga aggttcccgg
     1501 gttaccaaac agggcagcaa cccctatact ggccattacc ttgcttccgt gaagcagaat
     1561 ttcacgccgt gaaggaccga cttcgctcgt attgacgaca aggcttgcct cattgagttc
     1621 ttcgtcgcca tgatcggctt ccttatttgc tgaatcggtc atctaagcct acgtgcttga
     1681 gtgcttcaca cgaaattccc gaccaatgta taccactccc tacatcttcc tcaaagtaag
     1741 accttggctc ttggacaata cagttacaga agtccgcagc gttgatgtga tacgctagct
     1801 gccggtcctg tgtttgccgt ctttttttcg tggctggcgt cgtgagagac ccgcatgtcg
     1861 atggattcaa tccacgaagt cgttgagcct gtggtcgacc cggaattcgg gccatggttg
     1921 gcccaagcgt cgattctggt cgtcgatgat gaaccgggga tgcggaactt cctgctcaag
     1981 acactcgggt cacgttgtcg gtcgatccag gaggcgtcgg acacgaagga agcgtcgcgc
     2041 aagctcgacg ctcagcattt cgatgtcgtg atcctcgata acgtgatgcc gggcgaaaac
     2101 ggtctcgatt ggcttgccaa acagcgggcg atcggcctct tctccgacgt gattctcatg
     2161 accgcgtatg ccgatctgga caccgctata gaggctttgc gggccggcgt ggtcgacttc
     2221 gtcctcaagc cgttccgatc caaccaactg ctgaacgccg tggcacgttg tcttgatcgg
     2281 acgcggctgc aacgcgagaa ttacgtgctg cgtcacgaac tgaagtcgct ctcacatcag
     2341 gtcttcctgc ggagcaaact gctcggcgac tccgtcgtaa tccaacaggc gcgcgaagcc
     2401 atcgcgcgcg tcgctcctct gccgacttcg gtattattca ccggccaatc gggaaccggc
     2461 aaggaagtgg ccgcccgttc gctccattcg ctttccaatc gcgccgacca ggtgttcgtg
     2521 ccgatcaact gcggtgcgat ttctcccgat gtgatcgaga gcgaactgtt cgggcatttg
     2581 aagggagcgt tcaccggtgc gagcagacca cgtgatggtc tgttcatgca tgcgcagggc
     2641 ggcaccctgt ttctggatga gatcggcgaa ttgccctaca tgctgcagag caagctgctg
     2701 cgcgtactgg aggatcgccg cgttcggccg gtcggctcgg agcgcgagat tccgttcgac
     2761 gctcgtttcg tcttcgccac caacaccgat ctgaaaggcc gcgtcaaagc aggcaccttc
     2821 cggtccgacc tctacttccg gatcaacgtc atgcagattc atctgccgcc gctgagggat
     2881 cgcggggacg acgtccagga actcgccgcg ctgttcatgc gcgagattgc tcaacagcta
     2941 ggcatgcccc ctgttacgat cgacgagagg atacgcgagg ctcttgcgcg gtatgactgg
     3001 cccgggaaca tccgggagtt gcgaaacctc atcgagcgaa cggtgattct tggggccttt
     3061 ccggaggact tcgagagatt cgtgcatgac gacgatacag gggggcagag tctcgccgaa
     3121 gtggagcaac ggcacatcgt gtctgtgctt cgcgaggcgg ggggcgatcg ggaagaagcc
     3181 gcgcaacggc ttggcatttc acgcaagacg atcgaccgca aatgcgcaag ctggcggata
     3241 tagaactgag gtcttgggga aaccggcatt cgctccgcca ccgtctgctc acgatcgcac
     3301 ttctgccgat gctcgttatt ccacctctgc tgctcgggat cagcatttat cgttggaacg
     3361 ccaagtttga
//
LOCUS       DATMTI010000578         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_318850, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000578 DATMTI010000000
VERSION     DATMTI010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318850"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..854
                     /locus_tag="VN039_09225"
     CDS             303..854
                     /locus_tag="VN039_09225"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HWV46184.1"
                     /translation="MNNLNKTTAHEHMRVEMDKLIASLDLPELNKEFMRVRWLELVIW
                     MDGKAQESVWWYRRLRLATIIGGVIVPALVSLNVGDDVAPLVKMLTFIVSLAVALSAA
                     VEEFFRYGERWRHYRRVTESLKSEGWQFLQLSGTYANHTHVQGYPAFAARVEELSREE
                     VESYITTVFTEKKEADEKTPSVP"
     gene            890..>1653
                     /locus_tag="VN039_09230"
     CDS             890..>1653
                     /locus_tag="VN039_09230"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWV46185.1"
                     /translation="MAKRAVLIGINKYQVPGADLQGCVNDVKNLSGALKTYYGFIDKD
                     IVMLTDAQATKKAMERAIKKLIASGKKGDVLLLHYSGHGSNVPDDNGDEADHRDEILC
                     PTDLDWKDPFRDDWLRKTLGKLRSGVSLTVIMDCCHSGTITRVMNPLDSSRRQRFLPC
                     PLDLMATESGRKLRGTVRGRLSKVPRGRKRKNDVVQADIQELLITGCRDTQTSADAHI
                     GGSYNGALTYYLVESIKEAEGKLTYRELHQRTTAKL"
BASE COUNT          418 a          416 c          476 g          343 t
ORIGIN      
        1 tccattgagt cgattgtctg caaggaaagg gactgttggg ctaaccacgt aggccgcagt
       61 gcccaggcta ttctcaagaa ccgcgagtag agaacacgat ggcagaccta atcctagctc
      121 agcatctaca ggaacaccaa aggcttgatt gaacaatgcc tcatgccttc agaaacagac
      181 aagctttagt ttcttaacca ttcctactta tggtatctgt accagtacac atcggtatct
      241 cgtttgctac ctcgttttgg cagtgacctt ctctcgccgt gcacgactcg atggaggagc
      301 ctatgaataa tctgaataag acgactgccc acgaacacat gcgggtcgag atggacaagc
      361 tcatcgcatc actggatttg ccggaactga acaaagagtt tatgcgggtg cgatggttgg
      421 agctggtcat ttggatggat gggaaggcac aggagtccgt gtggtggtat cgcagactgc
      481 gtctggcgac gatcatcggc ggcgtgatcg taccggcgct ggtcagcctg aatgtcggcg
      541 atgacgtcgc acctctggtc aaaatgctca cgttcatcgt cagccttgcg gtggcgttga
      601 gtgccgccgt ggaggagttc tttcgatacg gcgagcggtg gcggcactac cgccgggtga
      661 ccgagtcgct caagagcgaa ggctggcagt ttttacaact gagcggaacc tatgccaacc
      721 atacacatgt tcaaggatac cccgccttcg cggcgcgggt tgaagaattg agccgtgagg
      781 aggtcgagtc ctacatcacg acggtgttca cagaaaaaaa ggaggcagat gaaaaaacac
      841 cctctgtgcc gtgaagtcgg tacctattca atcacagaaa ggagtgcgta tggccaaacg
      901 agcggtgctc atcgggatca acaagtatca ggtgccgggg gctgatctcc aaggatgtgt
      961 caatgacgtg aagaatctga gcggagcgct gaaaacctat tacggcttta tcgataagga
     1021 catcgtgatg cttacggatg cccaggcaac gaaaaaggcg atggaacgag cgatcaagaa
     1081 actgattgcg agcgggaaga agggtgatgt gctgctcctc cattattccg ggcatgggtc
     1141 gaacgtgccg gacgacaatg gcgacgaagc cgatcatcgg gatgaaattc tgtgtccgac
     1201 cgatctggat tggaaggatc ccttccgcga cgactggctg aggaagacct tgggaaaact
     1261 tcggtctgga gtgagcctca ccgtcatcat ggactgctgc cactccggca ccatcacgcg
     1321 cgtgatgaat ccattggact cttccagaag acagcgattt cttccgtgcc cattggatct
     1381 catggcgacc gaatccggcc ggaaactacg tgggacggtt cgtgggcgac tgagcaaggt
     1441 tccgcgagga cgcaaaagaa agaacgatgt cgttcaggcg gatatccaag aactgctgat
     1501 caccggctgt cgggatacgc aaacatccgc cgatgcgcat atcggcggct cctataatgg
     1561 agccctgacc tactacctcg tcgagtcgat caaagaagcc gaaggtaagc tgacctaccg
     1621 cgaattgcat caacgaacca ccgcaaaact gaa
//
LOCUS       DATMTI010000579         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_742795, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000579 DATMTI010000000
VERSION     DATMTI010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742795"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(470..1171)
                     /locus_tag="VN039_09235"
     CDS             complement(470..1171)
                     /locus_tag="VN039_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46186.1"
                     /translation="MKLPDRIYHLAEAANWPSIQRHGLLSAGRLMEVAGLTRADRDRL
                     GRTQRPAHTELCNGVQIRDQRPMSPTALAHCLRDMEPADWYAMINTRVFFWLDVERLN
                     RQKAACEPRPQVVMAVETAALVAAYEERVAVTPINTGNARRKPARRGVATFVPLAEWV
                     RSGWASEAALGISSRGRSHQPVELTVADAVPDIMRFVVGIVALSSGQSFEERKREGKV
                     DAALINSVRIRRVDG"
BASE COUNT          364 a          460 c          364 g          366 t
ORIGIN      
        1 gatgttatag gcagcctgtc gggtcatatg aggtataaat cgctatctat gccgatttga
       61 agttcttatg attcatagct gttcacggtg actctaactg gaactctgcg gtatgtgtca
      121 accctaagac tacgcttttt gatcctgctg ccgcgatact cacagaatgt tgtcgctcac
      181 tcgccggaaa gctgtctaat cttggttcca caggttgcgc tatgacgcgc aatcagactg
      241 caattctcag cacattccca cccaatgtca tgtagtgctc cccctttgtc aagcggccag
      301 gaactgaggg ctgcggctgc ggaagcgtgg tcattcctgg atacagcaca atggcgatct
      361 gcgtggtgtc cttcttcagt atcgagccga ctaccggatt aatttacctc tctattctag
      421 atgagtacag ctcgggaaag ctctacatta cacaggagat tgcgtgagat catccatcaa
      481 ctctccgtat ccgcactgag ttgatcagag cggcatcgac cttaccttcc cttttccttt
      541 cctcaaacga ctgaccggag gataaggcaa cgatcccgac aacgaaacgc ataatgtcag
      601 gcactgcgtc ggcgacggtc aactcgaccg gttgatgcga cctccctcgt gaagagatgc
      661 ccaatgctgc ctcgctcgcc catccggacc tgacccactc cgctaacggg acgaacgtgg
      721 caactccacg tcgcgccggc tttcgacgcg cattcccggt attgatcgga gtcactgcca
      781 ctcgttcctc atacgcggca accaaagcag ccgtctccac tgccatcacg acctgaggcc
      841 tcggttcaca ggcagctttc tggcggttga gtcgctccac atcgagccag aagaacacgc
      901 gcgtgttgat catcgcatac caatcggccg gttccatgtc acggagacaa tgggccagtg
      961 cggttggcga catcggacgc tgatcgcgaa tctgcacccc attgcacagt tcggtatgtg
     1021 ccggccgttg cgtcctcccc agtcgatctc gatccgctct ggtcagtccc gcaacttcca
     1081 tcaaccggcc ggcagatagc aacccgtgac gttggatcga cggccagttc gcggcttccg
     1141 ccaaatggta aatccgatcc ggcaacttca tatggaattt tctcactttc gtgattgaat
     1201 ggacaccaac ccacatagtg tgaccgaact tgtcctcgcc gagtaattct gacagttcag
     1261 aggaacatct tacaattcgc gttccatcta gaacgctctt ccacgtttta cacccataat
     1321 gactgattgt tccaataccg gatatcgttc gcgaaaaaaa tcatggggaa actgaaaccg
     1381 agtacgaatc ctcgcataat cgaaccagtc gcaacggagg tcacatatgc aggccaacat
     1441 gaatgagggt aacctcgaca cccctcctcg cgctctagaa aacaacgcat gtcttatttt
     1501 ccttcacatg cacaaccggt tcaaggaaag acagttcctc gttaagcagg tccg
//
LOCUS       DATMTI010000580         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_361485, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000580 DATMTI010000000
VERSION     DATMTI010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361485"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1885
                     /locus_tag="VN039_09240"
     rRNA            <1..>1885
                     /locus_tag="VN039_09240"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          457 a          466 c          628 g          334 t
ORIGIN      
        1 gtagcgaaag cgagtcctaa gagggcgacc atagtcgcac gaccaagacc cgaagcggag
       61 tgatctatcc atgggcaggt tgaagcgcgg gtaagaccgc gtggaggacc gaacccacca
      121 gggttgaaaa cctgggggat gacctgtggg taggggtgaa aggccaatca aactcagtga
      181 tagctggttc tccccgaaat gcatttaggt gcagcgtcgc gtgtttcttg ccggaggtag
      241 agcactggat ggtctagggg gcctacaagc ttaccgaaat cagccaaact ccgaatgccg
      301 gtaagtgaga gcgcggcagt gagactgcgg gggataagct ccgtagtcga gagggaaaca
      361 gcccagaaca ccggctaagg cccctaagcg tgtgctaagt gggaaaggat gtggagtcgc
      421 acagacaacc aggaggttgg cttagaagca gccacccttg aaagagtgcg taatagctca
      481 ctggtcaagt ggttccgcgc cgacaatgta acggggctca agcacaccgc cgaagccgtg
      541 tcattcacgc actagctcgg cgctgccttc gggcggcgtc caggcgcgtg gatgggtagg
      601 ggagcgtcgt acggccagcg aagcagcgga gtaatccagc tgtggacgcc acacgagtga
      661 gaatgcaggc atgagtagcg aaagaagggt gagaaaccct tccgccgaat gaccaagggt
      721 tcctgggcca ggctaatccg cccagggtaa gtcgggacct aaggcgaggc cgacaggcgt
      781 agtcgatgga caacgggttg atattcccgt acccgcgata gcacgaccat gctgaatccg
      841 gtgatgctaa gcgcatgaaa ccggcgagcc cttcggggtg agctggcgga gcgcgcgaac
      901 cgaaccggta ctaggcaagc aaaggggtga cgcaggaggg cagctcagcc cgggcgttgg
      961 ttgacccggg gtaagggtgt aggacgggcc gtaggcaaat ccgcggtcca tatgtctgag
     1021 acctgatgcc gagccgatta ggcgaagtga gtgatcccat gctgccgaga aaagcctcta
     1081 gcgagtgttg ttgcggcccg taccccaaac cgactcaggt ggtcaggtag agaataccaa
     1141 ggcgatcggg ctaactgtgg ttaaggaact cggcaaaatg cccccgtaac ttcgggagaa
     1201 ggggggccgt tcgacgtgta gggacttgct cccgaagcgt tggccggccg cagagaccag
     1261 gctgaagcga ctgtttacta aaaacacagg tccgtgcgaa gttgcaaaac gatgtatacg
     1321 gactgacgcc tgcccggtgc tggaacgtta aggggacggg ttagtggcct tcgggctgcg
     1381 aagctcagaa cttaagcgcc agtaaacggc ggtggtaact ataaccatcc taaggtagcg
     1441 aaattccttg tcgggtaagt tccgacctgc acgaatggcg taacgacttc agcgctgtct
     1501 caaccacaga cccggcgaaa ttgcactacg agtaaagatg ctcgttacgc gcggcaggac
     1561 ggaaagaccc cgggaccttt actacagctt gatattggtg gtcggttcgg cttgtgtagg
     1621 ataggtggga gactgtgaag cccggacgcc agttcgggtg gagtcaacgt tgaaatacca
     1681 ctctggtcgt actggctgtc taacctcggt ccgtaatccg gatcagggac agtgtcaggt
     1741 gggtagttta actggggcgg ttgcctccca aaaggtaacg gaggcgccca aaggttccct
     1801 cagcccggtt ggcaatcggg tgtcgagtgc aagtgcacaa gggagcttga ctgtgagaca
     1861 gacatgtcaa gcagggacga aagtc
//
LOCUS       DATMTI010000581         3504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_403717, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000581 DATMTI010000000
VERSION     DATMTI010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403717"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VN039_09245"
     CDS             complement(<1..382)
                     /locus_tag="VN039_09245"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46187.1"
                     /translation="MASTMSYVQLESALKQQGLGSLYLVVGEEDLLRDSALASVKRAA
                     LGSEGDEFNYELFYGDEAGGADIRNSVAAVPVFAERRLVVVKAAEKLNARESELLLDC
                     VKNPIDSTTLVFVSPKLDGRLKFSQ"
     gene            complement(401..1027)
                     /gene="lptE"
                     /locus_tag="VN039_09250"
     CDS             complement(401..1027)
                     /gene="lptE"
                     /locus_tag="VN039_09250"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWV46188.1"
                     /translation="MSRNRERRGPFFPILSTLVSALIASTVAACGYQFRVEGAGPTIG
                     GTSATSSQEPPPRLIIRTLINGSFEPNLETRYTNYLREEFSTGSGAQVVPESEAADLV
                     LTGQILSVIMPTLSFSRTTTLESRTEVVVLVRVEDARLRKVVWSQVVKGASEFFITSD
                     LQFNRALQNRAIEQAGRFVAADLAARFLLQLETGALTKQASAPVPVSH"
     gene            complement(1058..3493)
                     /gene="leuS"
                     /locus_tag="VN039_09255"
     CDS             complement(1058..3493)
                     /gene="leuS"
                     /locus_tag="VN039_09255"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWV46189.1"
                     /translation="MSKTFDHHAIESKWQAYWEQHRPFKASEDLSKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMRGFNVLHPMGWDAFGLPAEQYAVKTGIHPAITT
                     AENIATFKRQMKRVGLSYDWERELSTMDPQYYRWTQWIFLKLFERGLAYVAEVPVNWC
                     PALGTVLANEEIVDGKSEVGGFDVIRKPMRQWVLKITAYADRLLEDLKLVDWPSSTLE
                     MQKNWIGRSIGAEVDFSLADRTGVIRVFTTRPDTLFGATYMVLAPEHPLVDVVASGEQ
                     KTTVHAYREIAAKKSDLQRQELDKEKTGVFTGGYAVNPVNQERLPIWIADYVLMSYGT
                     GAIMAVPAHDERDWAFARRYDLPIREVISGGNVRQAAFTDTESGRVVNSASSDGSFSI
                     NGLTPSEAIPKITDWLGKQGKGTRAVNYKLRDWLFARQRYWGEPFPVLWVDGEARPLP
                     EEQLPLVLPETGNFKPSGTGESPLANLDAWLTTIDPATGKPARRETNTMPQWAGSCWY
                     YLRFIDPKNSIQLVDPVKERYWMPVDLYIGGSEHAVLHLLYARFWHKVLFDIGVTSTP
                     EPFLKLVHQGIVLGEDNQKMSKSRGNVVNPDEMIDQFGADAVRLYEMFMGPLEAMKPW
                     STRGVEGVTRFLERVWRLMVDEQGRLSPAIVSTAPSLDHQRLLHHTIKKVTEDIDALR
                     FNTAISQMMIFTNEMTKAQQRSRGVIEPFILLLSPFAPHVAEELWGMLGHQPSVSQQP
                     WPIFDPAMTVSEHMTIPIQVNGRLRAKLDVAVDTSRDAIERLARAAAAEWLQDKEPKK
                     VIYVEKKLLNFVI"
BASE COUNT          727 a         1047 c          948 g          782 t
ORIGIN      
        1 cctgggaaaa tttcagccga ccatccagct tcgggcttac aaacaccagc gtcgtggagt
       61 ctatgggatt ttttacgcag tcgagcagga gttcgctttc tcgtgcattc agtttctctg
      121 ccgccttcac cacgaccaag cggcgctccg cgaagaccgg tacggccgcc acactgttcc
      181 gaatatcggc tccgccggct tcatctccgt agaacagttc gtaattgaat tcgtcccctt
      241 cactgccaag tgccgcgcgt ttgacggatg cgagcgcaga atcccgtaga aggtcttctt
      301 cccctacgac gagatacaga gacccgagtc cctgctgttt caacgccgac tccaactgca
      361 cataactcat ggttgatgcc attggtccag gtcctcgatg ctagtgagac acaggaaccg
      421 gggcggatgc ttgcttcgtg agcgcacccg tctcgagctg taagagaaac cgtgccgcca
      481 ggtcggccgc cacaaagcgg cccgcctgtt cgattgcccg attctgtagc gcgcgattaa
      541 actgaagatc ggacgtaatg aagaactccg aggccccttt tacgacttgg ctccagacca
      601 cttttcttaa ccgggcgtct tccactcgaa ccaagacgac tacctcggtt ctgctttcca
      661 atgtcgtcgt ccgtgaaaag ctgagagtcg gcataatcac cgaaaggatt tgcccggtca
      721 gcacaaggtc cgccgcttcg gattcaggca cgacctgcgc cccgctgccg gtggaaaatt
      781 cttcgcggag gtagttggtg tatcgcgttt ccagattcgg ctcaaaacta ccgttgatca
      841 atgtccggat gatgaggcgt ggcggcggct cttgcgaaga ggtcgcggaa gttccgccga
      901 tggtcgggcc ggctccttcc actcggaact ggtagccaca ggcggcaacc gtcgaggcga
      961 tgagtgctga gacgagagtg ctgagaatag gaaagaacgg ccctcttctc tctcgattcc
     1021 tgctcactgt gaactcagca cccaacactc agcaccccta gatcacaaag ttgagcaact
     1081 tcttttcgac atagatcacc tttttcggct ctttatcttg gagccattcc gccgccgctg
     1141 cacgagccag ccgttcgatc gcgtcgcggg acgtgtcaac tgcgacatcg agtttggccc
     1201 tgagcctccc gttgacctga atgggaatgg tcatgtgctc gctcaccgtc atcgccggat
     1261 cgaagattgg ccaaggctgc tgcgagacac tgggctggtg tcccagcatg ccccaaagtt
     1321 cctcggcgac atgcggcgcg aacggcgaca acagcaagat gaacggctcg atcacccctc
     1381 gagaccgttg ctgcgccttc gtcatctcat tggtgaagat catcatctgc gagatggcgg
     1441 tattgaatcg gagggcgtcg atgtcctccg taactttttt gatcgtgtgg tgcagcaggc
     1501 gctgatggtc gaggctcggt gcggtcgaca cgatggcagg cgacagtcgg ccttgctcat
     1561 cgaccatcag ccgccagact cgttccaaaa aacgggtcac gccttctacg cccctggtgc
     1621 tccaaggttt catcgcctcg agggggccca taaacatttc atagagccgc accgcatccg
     1681 caccgaactg atcgatcatc tcatccgggt tgaccacgtt gccgcgtgat ttcgacatct
     1741 tctgattgtc ttctcccaag acgatgccct gatgcaccaa cttcaggaat ggttccggag
     1801 tgctcgtcac accgatatcg aacaggactt tatgccaaaa ccgagcatac aggagatgca
     1861 gaacggcatg ctcgctgccc ccgatgtaca gatccacagg catccaatag cgctctttta
     1921 ccggatccac gagctggatt gaattcttgg ggtcgatgaa tctcaggtaa taccagcaag
     1981 agccggccca ctgaggcatg gtgttggttt cacgccgtgc cggcttaccg gtggcaggat
     2041 cgatcgtcgt cagccacgca tccaagttgg ccaatggact ctcaccggta ccggacggct
     2101 tgaagttgcc ggtctccggt aagacgagag gcaactgctc ctccggaagc ggacgtgcct
     2161 ctccatcgac ccacaggacc gggaacggtt cgccccaata gcgttgtcgg gcgaagagcc
     2221 aatcgcgtag cttgtagtta accgccctgg ttcccttgcc ttgcttcccc aaccagtcag
     2281 taatttttgg gatcgcttcg gacggtgtga gcccgttgat cgagaaactg ccgtcgctgc
     2341 tcgctgaatt cacgactctg ccgctctcag tgtccgtgaa ggccgcttgc cgcacattcc
     2401 cgcctgaaat cacttcgcga ataggcagat catagcgacg cgcgaacgcc cagtctcgtt
     2461 cgtcgtgcgc cggcacggcc atgatcgcgc cggtcccgta actcatcaac acataatcgg
     2521 caatccaaat cggcaagcgt tcctggttca ccgggttgac ggcgtagccg ccggtgaaga
     2581 ctccggtctt ctccttatcg agctcttgtc gttgcaaatc gcttttcttc gcagcgattt
     2641 cacgataggc atggacggta gtcttttgct ccccactcgc gaccacgtcg acaagcgggt
     2701 gttcaggggc caagaccata taggtcgcgc cgaaaagcgt gtccggtcta gtcgtgaaca
     2761 ctcgaatcac gccggtccgg tcggccagtg aaaaatcgac ctctgcgccg atcgatcgcc
     2821 cgatccaatt tttttgcatt tccagcgtac tggaaggcca gtcgacgagt ttcaagtcct
     2881 ccagcaatcg atcggcatag gcggtgatct tgagcaccca ttgccgcatc ggcttgcgaa
     2941 tcacgtcgaa accgccgact tcgctcttgc cgtcgacgat ttcttcattc gccagcaccg
     3001 tgccgagcgc cggacaccag ttcacaggaa cttccgctac ataggccaat cctcgttcga
     3061 agagcttcag gaaaatccat tgggtccagc gatagtactg cgggtccatg gtactgagct
     3121 ctcgctccca atcatatgag agccccacgc gcttcatttg gcgcttaaag gtggcgatgt
     3181 tttcggcggt tgtgatggcg ggatgaatgc ctgttttcac cgcatactgc tcggccggca
     3241 gaccgaacgc atcccagccc atcggatgca gcacattgaa cccacgcatc cgtttgtatc
     3301 gtgagacaat atcggtggcg gtgtaccctt caagatgacc gacgtggagc ccggaaccgg
     3361 acggataggg gaacatgtcc agacaataaa acttgggctt cgataggtct tcggaggctt
     3421 tgaatgggcg gtgctgttcc caataggcct gccatttcga ttcgatggcg tgatggtcga
     3481 aagtcttact catggtccgg agaa
//
LOCUS       DATMTI010000582         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_192016, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000582 DATMTI010000000
VERSION     DATMTI010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192016"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1110
                     /locus_tag="VN039_09260"
     CDS             310..1110
                     /locus_tag="VN039_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46190.1"
                     /translation="MFVDDAALSNSAEALLQSPYFRPGRNGTIVSLCPDSEPFKDERS
                     TEKVLCVLRAVLSLGNPVQIPTKEKVPEAALEVIGTHAYPNQVVLFTSFSSLAKAGKI
                     EPRAAPVADRIRNFALARKHGVFVCMYLKPFLPATLKDLDHVLQVLEAERPDAICVGM
                     LYTTTQQQDSAYTHPVHASLTARPLDDDFNIFVAAVKAQGLVPLFHSSVCVTAWALDR
                     IPMPHIWKEYPALCVNCRNCERDLQDRFSQSAIPSFTSPQPVYHVSLP"
     gene            1094..2218
                     /locus_tag="VN039_09265"
     CDS             1094..2218
                     /locus_tag="VN039_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWV46191.1"
                     /translation="MSVYLNNATTGYPKAISAVEAYSAALQSIPSDVRHSAEPQIELV
                     RGRIASLLGVDHTHLFFLSDATLALNAVIRGYVGAGDHCVTDNRSHNAVLRTLNGIPG
                     VSWQVARVHDLHEEPDFSFLDVIDEPKLVCLTHTSNVTGSVYDVTTLIRRIRSRFPKA
                     AVLVDASQSVGFCPIDDLDDADFIVFPAHKHLHSMPGTAVLVARRRLVPFILGGTGSN
                     SAELALGDTGGNFVEVGTPNLPAILALGAALEEQNDGAHDIRRRIENLTEMLWSGLQS
                     VPGIQPLGRRPYQRRSGVVACKLPGSPEADWIPFLRSQDIIARGGLHCAPTIHEELGL
                     GNVGTLRFSISRFTREDEIRSAVEAVQIFADFVAEETGRP"
     gene            2215..2520
                     /locus_tag="VN039_09270"
     CDS             2215..2520
                     /locus_tag="VN039_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46192.1"
                     /translation="MTLYRSDRLVIEEPRPCAEEPDRVHLVGHLRSTTLDEIATLLLE
                     DRWRTLRLKQPAALVAHGPDGELLTLNAECEFSLSRLIDAQSGLAFFHDLITRMELQ"
     gene            2520..3173
                     /gene="pyrF"
                     /locus_tag="VN039_09275"
     CDS             2520..3173
                     /gene="pyrF"
                     /locus_tag="VN039_09275"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWV46193.1"
                     /translation="MSKFLGEKRTGLIFAADLPSFDENARVLDVISEYVDVIKVCNPL
                     VMREGAATIRKLADRYGKPVFADLKVADVPHTSAQIVKIVADNGGSAVMVHGFVGPDG
                     ISECMEAAGDNVGIIVQLELTNPGGKLFTAPIANDMARLAADMGVYGTQAPGNRPNRI
                     AEIRDIIGPDPVIVCCGVGAQGGKHNEVMQAGGTYSIVGRAIYNAEDPVQAVTAVLN"
     gene            complement(3354..>4207)
                     /locus_tag="VN039_09280"
     CDS             complement(3354..>4207)
                     /locus_tag="VN039_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV46194.1"
                     /translation="LQAYRIGHGLSPEEMAKRLGISRAALYRAEKGEILKIELLVSIT
                     KVLGVSIQSLLGVDIEYIDTAGSFFERMRQLEEKSEQIIGFFGPISYLLTSEVYDEML
                     QEVFLDTAPMDDATKDAIRRLIDTLKGRKKNYRLRRPLLVSLIASSDLERFLLHGLIG
                     RQDLPEEVQRRRRVMACREVRHISSLFRKQPLGVQLGILPQSVPSTSFQIFRQADHSV
                     LAISPFRLGEQPNIRVGVGLITTSQDGVRLHEDISRQMWEQALKATEAADFIDNLIAK
                     YGLDDDR"
BASE COUNT          865 a         1255 c         1179 g          908 t
ORIGIN      
        1 atattgctcc ttcaggggca cccatccttc ttctgtcttt cccacgcgga gattgactgg
       61 tccgacctgt ccgcacgcat cgagcgggct ctgagggcat ctttgccatt attcgcctga
      121 tccaagaacc gcttgcagac cgccagtcgg tcatctgcct cgtaggagtt gcgaatgcct
      181 tttccagacc acatccattt tctcaatcag ggcgatgagc cgggcgaccg ggtgttcatc
      241 aatatcacga cacggggctg cggtagcggt tgttcctatt gctatatcat ctccccggcg
      301 ggaccccagg tgttcgtcga tgatgccgca ctttccaaca gcgccgaagc cttgcttcag
      361 tccccctatt tccggccggg tcgcaacggc acgatcgtct cgctctgccc ggattccgaa
      421 cccttcaagg acgaacgatc gacagagaag gttctctgtg tgctgcgcgc agtgctctcg
      481 cttggcaatc cggtacagat cccgacgaag gaaaaggttc cggaggctgc gctggaggtg
      541 atcggcaccc atgcctaccc taatcaggtc gtgctcttca cctctttctc gtcactcgcc
      601 aaggctggca aaatcgagcc acgggctgcc cctgtcgccg atcgaattcg taatttcgcc
      661 ctagcgcgca agcacggtgt gttcgtctgc atgtatctga agccattcct gcctgccacg
      721 ctgaaggacc tggatcatgt cctccaggtt ttggaggcgg aacggcccga tgcgatctgt
      781 gtcgggatgc tctacacaac cacgcagcag caggattcgg cctatactca tcctgtgcac
      841 gccagtctca ctgcgcgccc gctggatgac gatttcaata tcttcgtcgc cgccgtaaag
      901 gcacagggcc tcgtgccgct tttccattca tccgtgtgcg tcaccgcctg ggcgctggat
      961 cgcattccta tgccgcatat atggaaggaa tatccggcgc tgtgcgtcaa ctgccgcaac
     1021 tgtgagcgtg atctacagga ccgtttttca caatcagcta ttccctcctt cacctctccg
     1081 cagccggtgt atcatgtcag tctaccttaa caacgccacc acaggatatc caaaggccat
     1141 ctctgcggta gaggcctatt ccgcggcgct gcaatcgata ccatcggatg tgcgtcacag
     1201 cgctgagccg caaatcgaac tggtgcgggg gcgcatagcc tcgctgctgg gcgtggacca
     1261 cacgcatctc ttcttccttt cggatgcgac gctggcgctt aatgccgtaa tccggggata
     1321 tgtcggcgcg ggagaccatt gcgttaccga caaccggtcg cacaacgcgg ttctgagaac
     1381 actcaacgga ataccgggcg tcagttggca ggttgctcgg gttcatgatc ttcacgagga
     1441 acccgacttt tccttcctcg atgtcattga cgagccaaaa ctggtttgcc tgacccacac
     1501 cagtaacgtc accggttctg tttacgatgt gacgacgctc atcaggcgca tccgctcgcg
     1561 gtttccgaaa gcggctgtgc tcgtggatgc gtcacaatcg gtaggctttt gccccattga
     1621 cgatctggat gacgctgact tcatcgtatt tccggctcac aagcatctgc attcaatgcc
     1681 tggtacggcc gtgcttgtgg cacgccgcag gcttgttccg tttattctcg gcggtacagg
     1741 cagcaactca gctgaactcg cgctcggcga tactggcggg aatttcgttg aggttggcac
     1801 accgaattta ccggcgatac ttgcactggg cgcagcactt gaagagcaga atgacggtgc
     1861 tcacgacatt cgccgtcgga tcgaaaacct caccgagatg ctgtggagcg gacttcagtc
     1921 ggttcccggc atccagcctc tcgggcggcg accctatcag aggcgttcag gcgttgtcgc
     1981 ctgcaagctg ccgggctcac cggaagctga ctggattccc tttctgcgca gccaggacat
     2041 aatcgccagg ggagggcttc actgtgcccc gacaattcac gaagaactgg gtctgggaaa
     2101 cgtcggcacc ctccgcttca gcatcagccg gttcactcgt gaagacgaga tccgtagcgc
     2161 tgtagaggct gtgcagatat ttgcggactt tgtcgcagag gagacgggtc ggccatgacg
     2221 ctatatcgat ccgatcgcct tgtaatcgaa gaaccacgcc cctgcgcaga ggagcccgac
     2281 cgtgtccacc tggtaggcca tctaaggtcg acgacactcg acgagatcgc cacgcttctg
     2341 ctcgaggacc gctggcgaac cttgcgactg aagcagcccg ccgcactggt ggcgcacggc
     2401 ccggacggag aattgctcac gctgaacgcg gaatgcgaat tctcgctctc gcggctgatc
     2461 gacgcgcaat ccggccttgc attttttcac gatctgatca cgagaatgga gttgcaataa
     2521 tgagcaagtt tctgggagaa aaacgcaccg gccttatatt cgcggcggat ttgccctctt
     2581 tcgatgagaa tgcccgggta ctcgatgtca tcagtgaata tgtcgatgtt atcaaggtct
     2641 gtaaccctct ggtaatgcgg gagggcgctg caacgatccg caaactggca gaccgttacg
     2701 ggaagcctgt gtttgctgac ctcaaggtcg ccgacgttcc gcatacgagt gcccagatcg
     2761 tgaaaatcgt ggcggacaac ggcggttcag ccgtaatggt gcacggcttt gtcggtcccg
     2821 acgggatttc ggaatgcatg gaggccgccg gcgataacgt aggcatcatt gtccagttgg
     2881 aactgacgaa tccgggtggg aaattattta ccgctccaat tgccaacgac atggcccggc
     2941 ttgcagctga catgggggtc tacggcaccc aggcacctgg caacagaccc aacaggatcg
     3001 cagaaatcag agacattatt ggtcccgacc cggtcatcgt ttgctgcggc gtcggagccc
     3061 agggcggcaa gcacaacgag gttatgcaag ccgggggcac ctattccatc gtcgggcgcg
     3121 cgatctataa cgccgaggat cccgtccagg cggtcacggc tgtgctgaac tgaccctgga
     3181 ggccgtctcc atttgtggac ggcttctccc gttaactgcg cgctccggca gttgccggat
     3241 cggtccacag accatcagcc gccggagaac tacagtggct gatgcaaacc actccgcgac
     3301 aggaacccgc gcgggacgcg gcgtcgtcga aagaccggag gttccactcg accctaccgg
     3361 tcgtcgtcca aaccgtattt ggctatcaga ttatcgatga agtccgccgc ttcggtcgcc
     3421 ttcagcgcct gctcccacat ttgacgtgaa atgtcttcat gcagtcgcac gccgtcctgg
     3481 gaggtggtta tcagaccgac cccgacgcga atatttggct gctctcccaa acggaacgga
     3541 ctgatcgcca acaccgagtg atcggcctga cgaaaaatct ggaagctggt cgaggggacc
     3601 gattgcggaa gaatccccag ttgcaccccc aggggctgct ttcgaaacag gctgctgatg
     3661 tggcgcactt cccggcatgc catgacacgg cggcgcctct gaacttcttc cggcaaatcc
     3721 tggcgaccaa tcaggccatg gagaaggaaa cgctcaagat ctgaggaggc gatcaggcta
     3781 acgagcaaag gccggcgcaa tcggtagttt ttctttcgtc ccttcaacgt atcgatcaac
     3841 cggcggattg cgtctttggt cgcgtcatcc atgggagctg tatcgaggaa gacctcctga
     3901 agcatctcat cgtagacttc ggaggtcagc agataagaga tcgggccaaa aaaaccgatg
     3961 atctgctcgc tcttctcctc gagctgacgc atgcgctcaa agaaggagcc ggcggtgtca
     4021 atatattcga tgtcaacgcc aaggaggctc tggatagaaa cgcccaacac cttggtgatg
     4081 ctgacgagca gctctatctt gagaatctcc cctttctccg cgcgataaag ggcggcacgg
     4141 gaaatgccga gacgtttcgc catttcctcc ggcgaaagac cgtgaccgat acgataggcc
     4201 tgcaatc
//
LOCUS       DATMTI010000583         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_828555, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000583 DATMTI010000000
VERSION     DATMTI010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828555"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..346)
                     /locus_tag="VN039_09285"
     CDS             complement(200..346)
                     /locus_tag="VN039_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46195.1"
                     /translation="MAFATEVAVAGALAAQMFTAIDKEIPPGPPDVPKLLDVLKRNGV
                     TVAV"
     gene            complement(788..1492)
                     /locus_tag="VN039_09290"
     CDS             complement(788..1492)
                     /locus_tag="VN039_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46196.1"
                     /translation="MRVVGGIAAAITAIAIEACSMTGGGPTRTGIDQVPMYGGMDRSQ
                     YPDLRAADDKFITDVTAHYGSREKASVLLVEQGFVFYRQDNNAMAMRRFNQAWLLNPN
                     NPEAYTGFASVLFDDGKYCDAASMVDKAQSIGHVQDGFLPDAAFILVGCAMQGASPER
                     KTALLARSDELFVQALSSSAVQKAYTLTNWATAMYARGDYADAWAKVAELRRTTGKDM
                     SEQFLGRLSAKMPEPK"
     gene            complement(1657..2337)
                     /gene="traT"
                     /locus_tag="VN039_09295"
     CDS             complement(1657..2337)
                     /gene="traT"
                     /locus_tag="VN039_09295"
                     /inference="COORDINATES: protein motif:HMM:NF017619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HWV46197.1"
                     /translation="MSGCSNVIRSGLMNSNTIFLDPSTNQTIYTQLRNVSENQQVTLN
                     AVNTKLTAKGYQLAPDPEQANYWIQAKVIYCHKAADGVTPEGVAKVGPGEGISSGGTA
                     MVSTGSIGDETARGIAGMPDMGTIMRQAMAMSGGRAGFGGMSGFPNAQPLPKEDGVIY
                     LCVTDVQITDRKIGRPLGQPAGSQTAAVQKVQKMRMVGHVRQKSLDIPEATPIVQEKI
                     STGIAGLF"
     gene            complement(2421..3119)
                     /gene="traT"
                     /locus_tag="VN039_09300"
     CDS             complement(2421..3119)
                     /gene="traT"
                     /locus_tag="VN039_09300"
                     /inference="COORDINATES: protein motif:HMM:NF017619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HWV46198.1"
                     /translation="MRYLALLMVLGFPLPSHAGNIKDTDLISSNTFFLLPSTHRTVFI
                     QTRNSSDNQDVSFHDLGSRLVAKGYQVVNDPDSAQYIVLANIVYCNITKPEMPVEAMV
                     ASGYGSAFNSTLGAMQGLAGMASMAGPYGAMGGAAASMGLSAVQGISDLVGSSQSAPK
                     MPDDINYVCVADVQITEQGAVPIPAGMKAGTGQQPGVYQTRLAADVHQKKLNEAEATP
                     LLQQKLSAAVAGNF"
     gene            complement(3456..>3755)
                     /locus_tag="VN039_09305"
     CDS             complement(3456..>3755)
                     /locus_tag="VN039_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46199.1"
                     /translation="KHLGWAALKGTAKIAAGHLMRGQTNFVKMLWKFNSVYNPELQMA
                     DHRRSVVYEMTPPPEQKDKVDAKQLYILPAKGRQGRAIDDSTETFVDESRMGTAV"
BASE COUNT          795 a         1082 c         1017 g          861 t
ORIGIN      
        1 ggccgaagcg aggggtattg atcgagaatg cgttcaggcg tttcgcccgc agcgagatta
       61 tccagaacca ccgatacagg gattcgagta cctcgaaaac acacagcacc atgcatgatc
      121 tgcgagtcag tcgaaatgca ggacctccaa tccatcaccg acacctccga atatggccat
      181 caatatagtg cagtgttggt cacactgcaa cggttacccc gttgcgcttg agtacatcaa
      241 gcagctttgg aacatccggc ggtccggggg gaatctcctt gtcgatggcc gtgaacatct
      301 gagctgctaa cgcgccggcc accgccactt ccgtagcgaa agccatgcac ggttcctcgg
      361 agaacgtgca cgagagtccc aacagtgcag tcgtagacgt tcccgtcgca caggaagtga
      421 atctcgcctt tcaggacgta gaacgcttcg tcccaatcgt ggctatgcgg tggaggacct
      481 gttccttccg caccctgctg ctaaggtgat cccgtagctt tgcgtcgccg cgttcgacgc
      541 caacatagtc acctgtgttc cgaccatatt caatgcgggc tcatgctgat ccggtctgta
      601 agacaatggg ctgaacgctc atgacctcgc tgcctcctac aacgatggcg atgatgtgcc
      661 gcccaacgac ttagttgagc ggccagccat gaccgtcatt gcgccctggc gaacacacaa
      721 tccattgcaa gcagagggcg cccatgatga tccacggtcc gctccaacgc gttgttatac
      781 ggagacacta cttgggctcc ggcatcttcg cggaaagtcg cccgaggaat tgttcggaca
      841 tgtcctttcc cgttgtacgc cgcaactcgg ccacctttgc ccaagcatcc gcgtagtcgc
      901 cacgcgcata catggccgtg gcccaatttg tcagggtgta ggccttctga acggctgaag
      961 aggaaagcgc ttggacaaag agttcgtcag agcgcgccag taaagccgtc ttccgctcgg
     1021 gtgaggcacc ctgcatcgca cagccgacca aaataaacgc ggcatcaggt aggaaaccgt
     1081 cctggacgtg tccaatggac tgagctttgt cgaccattga cgccgcatca cagtactttc
     1141 cgtcatcaaa gagtacggag gcgaaccccg tgtatgcctc aggattgttt ggattcagga
     1201 gccatgcttg attgaaccga cgcatggcca tagcgttgtt gtcttgccga tagaacacga
     1261 agccctgctc taccaagaga acgctcgcct tctcccgcga cccatagtgc gccgtgacgt
     1321 cagtaatgaa tttatcatca gccgcgcgca aatcaggata ttgagaccgg tccatcccgc
     1381 catacattgg cacctgatca attcccgttc gcgtcggccc acccccagtc atcgagcaag
     1441 cttcgatggc aatggctgta attgcggcag cgatccctcc tacaactctc attttgtctc
     1501 cgtataacta tagtaagccg atcctcataa caaccggctc tgccagctct caaccgtata
     1561 acacgccgct actgttcgcg aactatacgc catgaccgca ttttcaatga gttgcggctg
     1621 caagaccatt gctacggggt tttacgtgga tcagagctag aacagccctg caattccggt
     1681 ggaaattttc tcttggacga tcggggtggc ttcgggaatg tctaaacttt tttgacgcac
     1741 gtggccgacc attcgcatct tctggacttt ttgaactgct gccgtttgac ttcccgccgg
     1801 ctgacctaat ggtctaccaa tcttgcgatc ggtaatctgc acgtcggtga cgcacaggta
     1861 gatcacacca tcttctttgg gcagcggttg cgcgttgggg aaaccggaca tgcctccaaa
     1921 tccagctcgt ccaccgctca tagccatggc ctgtctcatg atagtgccca tgtccggcat
     1981 accggcaatt cctcttgcag tctcgtcacc tatggatccg gtcgagacca tcgcagtgcc
     2041 tccgctgctg atcccctcgc cgggacccac tttagccacg ccttccggcg ttacgccatc
     2101 ggccgccttg tggcagtaga tcaccttggc ttgtatccaa taattggcct gctccggatc
     2161 tggagccagt tgatacccct ttgcggtgag cttcgtgttc accgcattca gcgtcacctg
     2221 ttggttctcg gagacgtttc tgagttgagt ataaatggtt tggttggtgc tgggatcgag
     2281 gaagatggta ttgctgttca taagaccgga gcggatgacg ttggaacagc cgctcatgag
     2341 accgcatgtt agcaataata gagcaagcgg cctcgtgaat cgtggggcgt gaggcgtgaa
     2401 gcgaacagac cagtgtgtca tcagaaattt ccggcgacgg cggcgctcaa tttctgttgc
     2461 agcaacggtg tcgcttcggc ttcattcaat tttttctgat gcacatcggc agccagtctg
     2521 gtctggtaga cgccgggttg ctgccccgtt ccggctttca tgcctgcagg aatcggcacg
     2581 gcgccctgtt cggtaatctg cacgtcggct acgcagacat aatttatgtc gtccggcatt
     2641 ttgggtgccg attgggaaga accgactaga tcgctgatgc cttggaccgc gctcaaaccc
     2701 attgaggccg ccgcgccgcc catcgcgcca taggggcccg ccatgctcgc catgccggca
     2761 aggccttgca tggcacccag tgtcgagttg aacgcagatc catagccgct cgccaccatc
     2821 gcttcgaccg gcatctcggg tttggtgatg ttgcagtaga cgatgttcgc caacacgatg
     2881 tactgcgcgc tgtccgggtc gttcacgacc tgatatccct tcgcgaccaa gcgtgacccg
     2941 agatcatgaa acgagacgtc ctggttatcg gacgagttgc gggtctgaat gaacaccgtc
     3001 ctgtgcgtgc tgggcagcag gaagaacgtg ttactggaga taagatccgt atcttttatg
     3061 ttgccggcgt gtgacgggag agggaaacct aggaccatga gcagagcgag gtaccgcatg
     3121 aacctgtcct ccctttcagt gaaggcgaat tctacgccag ccttccagac ctcagcaact
     3181 gcattcatag gcattgatgt accggcctca cacatctcag aacgactata aggtgaacaa
     3241 ccctccgcta tgccgagaaa atgcgcttcc tgagtgggtg ggctggaaga cgatcaatgc
     3301 gatgacgcgg gtcggcaaag aggaccgggt attcaagaag attatgagat gagaaggtca
     3361 ttgctcccaa gatggagcaa tgacttcatt tgaaagtggc aggagatcgg cgttttatcc
     3421 ggcaggcact cctgcgtcca ctacgtctac gagtgttaga ccgccgttcc catacgggat
     3481 tcgtcgacga aggtctcggt ggaatcgtcg atggcccgac cctggcggcc tttcgcgggt
     3541 aaaatgtaca gctgtttcgc gtcgaccttg tctttttgct ccggcggcgg cgtcatttcg
     3601 tagacgaccg accgacgatg atccgccatt tgcaactcgg gattgtagac gctgttgaac
     3661 ttccacagca tcttgacgaa gttggtctgt ccccgcatta aatgccccgc cgcgatcttg
     3721 gccgtgcctt tgagcgcggc ccagcccagg tgttt
//
LOCUS       DATMTI010000584         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_531362, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000584 DATMTI010000000
VERSION     DATMTI010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531362"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..415
                     /locus_tag="VN039_09310"
     CDS             14..415
                     /locus_tag="VN039_09310"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HWV46200.1"
                     /translation="MTTFFRYWVPVCGYAGLIFYLSAQSHPETRLPFVIHFSDKVLHV
                     AEYAVLGALCYRALRGSGYDSWRQQAIPAAILLASLYGVTDEVHQAFVPFRDSSWLDW
                     LADTAGASLGVAAMHRILNLRPMSSIPEALP"
     gene            582..1766
                     /locus_tag="VN039_09315"
     CDS             582..1766
                     /locus_tag="VN039_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWV46201.1"
                     /translation="MSDKAPTTDRIRGVRDKSRDARYNSLSMLSANTTPIKTPLSPPD
                     QTLIAQAVETHLPPGLLLRTLTPLAGDASNRRYYRAAIAGGPPHSVIVMQLAEPEGFK
                     QSEEAVSGSIHRISELPFINIMSHLAKAKVPVPTLHYYDQSAGLLYLEDFGDVTLTEA
                     VSQADAPGLESRYKQAINVLVQMQINATTPVDPGCMAFHRSFDSPLLMWEFDHFLEYG
                     IVARSGKPLPDKDATAIQREFEGIAKLLAGQPRVFTHRDYHSRNLMVDGLRLGVIDFQ
                     DALMGPATYDLASLLRDAYIQLDEALVDDLVEYYLDQSAERRFVWTNRAAFRRLFDLT
                     SIQRNLKAAGRFVYIDRVKGNPKFLADIPRVLSYVKRNLEKYPELDTLRKHLSPHVPE
                     LQ"
     gene            1856..>2491
                     /locus_tag="VN039_09320"
     CDS             1856..>2491
                     /locus_tag="VN039_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWV46202.1"
                     /translation="MILAAGLGTRLRPLTNTIPKPLLPVGGTPLIVWNLLLLKRHGFR
                     QVVINLHHLGPMIEQALGSGSKFGIRIMYSHEPVILGTGGGIKQAEPHFSGEPVLILN
                     GDTLAELDLEALCDFHRTRNAAATLALREDSDAARWGLVEVGDEGQILRITGKGLVGS
                     ASAASRMFAGIHILHPRLLRDVPKGVASSIIDPYVRAIERGEPVLGYDLQGY"
BASE COUNT          500 a          676 c          724 g          591 t
ORIGIN      
        1 gtgaggagtt gagatgacga cattttttcg gtactgggtg ccggtgtgcg gatatgccgg
       61 gctcattttt tatttatccg cgcagtctca tcctgaaacc cgtctgccgt tcgtcattca
      121 ttttagtgat aaggtattgc atgtcgccga atatgcggta ttaggtgcat tgtgctaccg
      181 ggcacttcgt ggaagtggat atgattcttg gagacagcaa gcgattccgg cggccattct
      241 actggcttcg ttgtatgggg tgaccgacga ggttcaccaa gctttcgttc cgtttcgaga
      301 ttcgagttgg ctcgactggc tggccgatac cgccggagcc tcgctcggag tggccgccat
      361 gcatcgcata ttgaatctca ggccgatgag ctcgattccg gaagcattgc cttaggagcc
      421 tgtccgacat tgaccttcta ctgcggcgaa ggatgcaccg gcatctgcgt cgttctcggc
      481 tccgaaaaat cctcgacgta ttccagtgaa tacgcctccg gtttttcgtg gcctgtggcc
      541 ttgcatctgc cagcgcctgc ttcgcctcgt cacgaacggc aatgtcggac aaggctccca
      601 ccaccgatcg aatacgtggt gttcgtgaca agtcgcggga cgctcgctat aattcgctga
      661 gtatgttgtc ggccaatacc acccctatca agactccgct gtctcctccc gatcaaacac
      721 tcattgctca ggcagtcgag actcatcttc ctcccggcct cttgttgagg acgctgaccc
      781 cattggcagg ggatgcctcc aaccggcgct actatcgagc cgccattgcc ggagggcctc
      841 cgcattcggt aattgtgatg cagctggctg agccggaagg attcaaacaa tctgaagagg
      901 cggtaagcgg gagtatccat cggatctccg aactgccgtt catcaatatc atgtcacatt
      961 tggccaaagc gaaggtgccg gtgcctacac tccactacta tgatcaatcc gcagggctgc
     1021 tctacctgga agacttcgga gatgtgacgt tgacggaggc ggtcagtcag gcggatgcgc
     1081 cgggcttgga gtcacggtac aagcaggcga tcaacgtctt ggtccagatg cagatcaacg
     1141 ccacgacgcc ggtggatccc gggtgcatgg catttcatcg cagcttcgac tcgccgttgt
     1201 tgatgtggga gttcgatcac ttcctggaat atggaatcgt ggcgcgcagc ggcaaaccat
     1261 tgcccgataa ggacgccacg gccatacaac gcgagttcga agggattgcg aaactactgg
     1321 ccgggcagcc gcgcgtcttc acgcatcgtg attaccactc ccgcaatttg atggtcgatg
     1381 ggctgcggct cggtgtgatc gactttcagg acgccttaat ggggccggct acctacgatc
     1441 ttgcttcact gttgcgggac gcctatattc agcttgacga agccctcgtt gacgatttgg
     1501 tggagtacta cctcgaccag tcggccgaac ggcgttttgt ctggaccaat cgtgccgcgt
     1561 tccgacgact gtttgatttg acgagtattc aacgcaatct gaaggcggcc ggccggtttg
     1621 tctacattga tcgggtcaaa ggcaacccga aattcttagc cgatattcct cgtgttttga
     1681 gctacgtcaa acgtaatctt gaaaaatatc cggaactgga taccctccgg aagcacctca
     1741 gcccgcatgt gccggaattg cagtagagga ctgaggggtg aggactgagg actgagcatc
     1801 ggatcatcga ctcagcactc agcactcagt cctcagcact gatcgtgtga aagccatgat
     1861 tcttgcggcc ggtctcggca cccgtctgag accgctgacg aacaccattc ctaaaccgtt
     1921 gctgccagtc ggcggaaccc cgctcatcgt ctggaactta ctgctgctga agcggcatgg
     1981 gttccgtcaa gttgtcatca acctgcatca tctgggtccg atgatcgaac aggcattggg
     2041 ttccgggtcg aaattcggga ttcggatcat gtactctcat gaaccggtca tcctgggaac
     2101 cggcggtggg attaaacagg ccgagcctca cttttcggga gagccggttc tgattctcaa
     2161 tggggacacg ctggcggaat tggatcttga ggcgctttgt gatttccacc gcacgcgcaa
     2221 tgcggcggca acgctggcac tcagggaaga ttccgacgct gctcgctggg gattggttga
     2281 ggtgggagac gaggggcaaa tcttgcgtat caccggaaaa gggctggtgg gctccgcttc
     2341 agcggcgtcg cgcatgttcg ccgggattca tatcttgcat ccccggttgc tccgggatgt
     2401 gccgaaggga gtagcctcct cgatcattga tccctatgtg agagccatcg agcggggaga
     2461 accggtgctc gggtatgact tgcaggggta t
//
LOCUS       DATMTI010000585         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_531417, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000585 DATMTI010000000
VERSION     DATMTI010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531417"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VN039_09325"
     CDS             complement(<1..605)
                     /locus_tag="VN039_09325"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HWV46203.1"
                     /translation="MIKIAAFFLLAAGSMAMAQTQNWQPDVTQQQTYTLHRASSADPT
                     GGNADFRTVAPGATCTVLDTDGPGMISHIWFTINDREAYHLKRIVLRIYWDGEATPSV
                     ETPIGDFFGLGLGTYHNWQSEMLSVGSIKALNSYFPMPYRKHARITVTNEGKDPIDSL
                     YYNVDYRTESHPLPEGTLYFHAQYRQAQPNHGWTNDWPNNYV"
     gene            complement(637..>1669)
                     /locus_tag="VN039_09330"
     CDS             complement(637..>1669)
                     /locus_tag="VN039_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HWV46204.1"
                     /translation="LIFFRFIGGVGIGGSSVLGPMYIAEIAPTEWRGRLVGFFQVNIV
                     VGILLAYVSNYLIGTMHLGAYEWRWQLGISALPALFFLLALFGIPRSPRWLSMKMKLD
                     EALHVLRLTGIPNPQEDLDEIVASIHLERSASADPLFSKRYRFPVFLAITVGMFCQLS
                     GINAILYYLNDIFAMAGASKVSGDLQAVAVGVANLVATLLAMSVIDKIGRKKLLLFGT
                     AGLALCLSAVSYVFLTHKHLDWLVWLLMIYIAFFAVSQGAVVWVYISEVFPTRVRSKG
                     QSLGSSSHWVTNAAISLVFPLMAVSSGAYPFIFFAAMMVLDFFLVLFYYPETSNISLE
                     HLQTSLGID"
BASE COUNT          440 a          454 c          451 g          324 t
ORIGIN      
        1 acataattat tcggccaatc attcgtccaa ccgtgattgg gttgtgcctg tcggtactgc
       61 gcgtggaagt atagcgttcc ctcaggaagt ggatgtgatt ctgtgcggta atcaacgttg
      121 taataaaggc tgtcgatcgg gtccttacct tcattcgtga cggtaatccg tgcgtgcttt
      181 cgatacggca tcgggaagta gctgttcagc gccttaatgc tgccgacgga gagcatctcc
      241 gattgccagt tatgataggt tcccaatccg agcccaaaga aatctccgat cggagtttcc
      301 acgctgggcg tcgcctcgcc atcccaatag atacgcagga cgatacgctt caggtgatac
      361 gcctctcgat cgttgatcgt gaaccagata tgcgagatca tccccgggcc gtccgtatcc
      421 agaaccgtgc aggtagcgcc gggagcaacg gtgcggaaat cagcatttcc gcccgtggga
      481 tcagcactcg aagcacgatg caaggtatag gtctgctgct gcgttacatc cggctgccag
      541 ttctgcgtct gcgccatcgc catcgaccca gcagccagaa gaaagaatgc tgcgattttt
      601 atcattatga ttacctcgtt gaattggaat cgaacgtcaa tcaatcccca ggctggtctg
      661 cagatgctcg agagagatat tgcttgtctc cgggtaatag aacaggacca ggaagaagtc
      721 gagcaccatc atcgccgcga agaatatgaa tggataagcg ccggaagaca cggccatcag
      781 aggaaatacc agcgagatgg cggcattggt gacccagtgc gacgaactgc ccaggctctg
      841 ccccttcgac cggacccgag tcggaaatac ctcgctgata tagacccaga cgaccgcacc
      901 ttgagacacg gcaaagaagg caatgtagat catgaggagc cataccagcc agtcaagatg
      961 tttatgggta aggaagacat acgaaactgc agacaggcat aacgcaagcc ccgccgtgcc
     1021 aaacagcaac agtttcttgc ggccaatctt gtcgatcacc gacatcgcca gcaatgtggc
     1081 tacaaggttc gcaactccca cagcgacagc ctgtagatcg ccggacacct tcgaagcccc
     1141 ggccatggca aagatatcgt tcagataata gaggatcgca ttgatccccg ataactgaca
     1201 gaacatgcct accgtaatgg caagaaaaac cgggaagcga taccgcttgg agaacaatgg
     1261 atcggccgaa gcagagcgct ccaggtggat cgaggcaaca atctcatcaa ggtcctcctg
     1321 cgggttgggg atgccggtaa gacgaagcac gtgtagcgcc tcgtcgagct tcatcttcat
     1381 ggaaagccag cgtgggctgc gtggaatgcc aaacagcgca agcaggaaaa agagcgcggg
     1441 aagcgccgaa attcctagct gccaacgcca ttcataggcg ccaaggtgca tcgttcctat
     1501 caaatagttg gagacgtagg ccagcagaat tcccacgacg atattgacct gaaagaatcc
     1561 gaccaggcgc cctcgccatt cggtgggcgc aatctcggca atatacatcg gccccagaac
     1621 ggacgatcca ccgatgccga cgcctccgat gaagcgaaag aagatcaac
//
LOCUS       DATMTI010000586         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_573254, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000586 DATMTI010000000
VERSION     DATMTI010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573254"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VN039_09335"
     CDS             complement(<1..666)
                     /locus_tag="VN039_09335"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HWV46205.1"
                     /translation="MPGTFLRARKPADVAKHGQPLYDVATFDPAPPGVVGGGRFNHAG
                     APMIYLATTATKALLEIGTLDELFSIAELALHGENRILDLVGLGDCSDVRIPILAMAN
                     SAWVAAPRIDPGWLRPEYLFSRFAADCPKSAGFDAVRYASTNLSEGENYVFALVQLQS
                     SELPPSDQCSKDRHRYRVSSSSYAMGHFTTGPSRMVMVMVAESQCNGFPTICLTAVQN
                     ASFA"
     gene            723..1310
                     /locus_tag="VN039_09340"
                     /pseudo
     CDS             723..1310
                     /locus_tag="VN039_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116569.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1303..1521
                     /locus_tag="VN039_09345"
     CDS             1303..1521
                     /locus_tag="VN039_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46206.1"
                     /translation="MTEKSILWEQDGIDSGWFFAKDVGSVRSNVSYRPGGWWFLAKWL
                     PDTEEHDVGPFKTKAAAMAEAERLAALQ"
BASE COUNT          377 a          452 c          497 g          350 t
ORIGIN      
        1 cgcgaaacta gcgttctgca cggctgtcaa acaaatggtg ggaaatccgt tgcattggct
       61 ttcggcgacc ataaccatca ccatccgaga tggacccgtc gtgaagtgac ccatagcgta
      121 agaactgcta cttacgcgat agcggtgcct gtcttttgaa cactgatcgg atggaggtag
      181 ctccgaagat tggagttgga cgagagcgaa aacgtagttc tctccctcac tcaagtttgt
      241 ggaggcatac cggacagcat caaatccggc gctcttgggg cagtctgcag caaaccgaga
      301 gaataggtat tccggtctca accagccagg atcaatgcgt ggtgcggcaa cccatgcact
      361 gttcgccatt gccagaattg ggatgcgtac atcggagcag tcgcccagtc cgaccaagtc
      421 gagaattctg ttctcgccgt ggagcgccag ctctgcgatc gaaaacagtt cgtcgagggt
      481 gccgatctcc agaagcgctt ttgtggcggt ggttgcgagg tagatcatgg gagcgccggc
      541 atgattgaat cgccctccgc caaccacacc tggaggagct gggtcgaacg ttgcgacgtc
      601 gtacaagggc tggccgtgct ttgcgacgtc cgcgggtttc ctcgcgcgga ggaatgtccc
      661 aggcatcaac ttgggagcag cgtctgcgga aaaatcgcag accgcgtcga ggatgcttcg
      721 cgcaaatgga tatggtcgtc gacatcgaga gccatgactg cccctgctgc agccacggcc
      781 tgcatcggat tggtgaagag gtcagcgaga agctcgccgt cgtgcctgcc aggatcgagg
      841 gcggcatccc gaccgaggcc acggtcgctc aggtgctagt gtcgagatat gccgatcatt
      901 tgccgctcta caggcaggcg caaatccata agcgtcaggg gatcgatcat gaccgctcga
      961 cactcgcgga ctgggtcgac cgcacgcctg gcatctgcgc ccgattcata gatgtcggcc
     1021 gtatcgagat cgactccaat acagtcgagc gtttgatcag gccgatcgct ctcaatcgta
     1081 agaacgcgct cttcaccggc tccgacgccg gagccgagca ctgtgcgacc attgcatcgc
     1141 tcatcgaaac ggccaagctc aacgacgttg atcagatggc ctatctgact gacgtcatca
     1201 ccaagatcgt caacggccat ccgaacagcc ggatcgatga gttgttgccg tggctatatc
     1261 gcttggataa agtggtcgga cacgtctacg agcaggtacc tcatgactga gaaaagcatc
     1321 ctttgggaac aggatggaat tgattccggc tggttctttg ccaaggacgt cgggtcggtg
     1381 cgaagcaacg tcagctaccg ccccggcggt tggtggttcc tagcgaaatg gctgcccgat
     1441 accgaggagc atgatgtcgg gccgttcaaa accaaagctg cagctatggc tgaagcggag
     1501 cgcctcgctg ccttgcagtg actagggaaa ccgtaaacca atgaacatcg cggtgttaga
     1561 acagtgcttc tgaaaagctg ttcaggggga tatctttaac gcacggccat ggcgttcggt
     1621 ttcttcctat tttttgcgac gacatatgga ttacagatgg aaaaggtcga gacgtt
//
LOCUS       DATMTI010000587         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_445963, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000587 DATMTI010000000
VERSION     DATMTI010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445963"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /gene="glk"
                     /locus_tag="VN039_09350"
     CDS             <1..756
                     /gene="glk"
                     /locus_tag="VN039_09350"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HWV46207.1"
                     /translation="IDGRTIAKQFNIPRVQLLNDLAATAYGLLWLRPDELEILNAGNP
                     PNKRQALALIAAGTGLGEGILFWDGKTYRPMPSEGGHTDFAPNNDQEIELLRYLRTQY
                     LHVSYERILSGPGLHAIYEFLRDTKKNEPTWLAEKIRAGNPAAEISQAGLQGQAEIAK
                     QALDLFTSIYGAEAGNLALKALSLDGVYVGGGIAPKLITKLQDGTFMKAFTNKGRYKR
                     LMSQMPVKVIMNQQTALIGAASVAAALSLASPS"
     gene            753..1451
                     /gene="rpiA"
                     /locus_tag="VN039_09355"
     CDS             753..1451
                     /gene="rpiA"
                     /locus_tag="VN039_09355"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWV46208.1"
                     /translation="MTSGNLDSFKRSAALKAVEFVRDGMIVGLGTGSTAKHLLIALGE
                     RVRAGMKLRGVPTSQETAMLARESGIPLIDTENRWEIDVAIDGADQVDPDFNLVKGGG
                     GALLKEKIVAARAKQFIVMVDYTKQVPVLGGSFPLPIEIIPFGWGSTAREVEVLTKSR
                     VILRECDGVPFKTEAGHLIVDVHIDRIDRPGELETALNLIPGVVETGLFVGRTSVLIV
                     GTPQGVHTLHVPGK"
     gene            1594..4176
                     /locus_tag="VN039_09360"
     CDS             1594..4176
                     /locus_tag="VN039_09360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HWV46209.1"
                     /translation="MSLDDVPDSHDLYRLPRHVIPTRYDLRLEPDLAGATFTGQATIT
                     ITVKQTTRIILLNAVDLVLRSAVAEDVNQRQVAALIELEQEAQRARFSFQEPVQTGEW
                     RLHLSFHGKLNDQLRGFYRSTYKDASGTPQTLAATQFEATDARRAFPCWDEPDFKAVF
                     ATTLVIDPYLTAVSNTSVTSERVENNKKVIRFADTIKMSTYLVAFVVGRMEATEPVWV
                     GKTPLRLWTVPGKQLLTPFGQDIAVASLKFFEDYYGIPYPGDKLDLVAIPDFASGAME
                     NLGAITFRETALLVDRQTGTHAELERIADVVAHENAHMWFGDLVTMAWWNGLWLNEAF
                     ATFMEMLAVDAWKPEWKRWETFGVARAAAFSVDGLVSTRPIEYPVHAPKDADAMFDIL
                     TYEKGASVLRMLEQHIGSTVFRDGVRQYLRTHAYGNADTKDLWTALGTVAGQPVPELM
                     DGWIFQPGFPLVTAEVRGQELLLSQQRFTYISQGQSAEQLWQIPIQIRLVIGDRTEHR
                     RLLSTERETTIGIPAGVTSVFVNEGGHGFYRVRYHGVLLQQLLDKGLDRLAAIERFAL
                     VSDAWATTVAGLTALPAYLRLTAHFKRERDKNVWAVLLDSFSFLSRIISSEDRGALET
                     FVRACVKPAIDELGWDAKPGESDLVRQLRGDLLGALGRLGNDAATQQEAANRYRRYRK
                     DPTTIDQNVVPALVAILAHTGDEARYDEFLELYRTAPTPQEERRYLFSLASFRHEELL
                     TRTLSRTINGEIRTQDAPFIVGALLTNVYGRELAWDFVKANWHHMDRLFPKQGLRRMC
                     GGIVGLVTPELEQDVRQFFTSRKIDLGGKTLDQYLEQLRVAVAFHEREGRTIKAALLS
                     SLQA"
BASE COUNT          956 a         1190 c         1213 g          905 t
ORIGIN      
        1 atcgacggcc gaaccatcgc caaacagttc aatattccgc gcgtacagtt gctcaacgat
       61 ctggcagcaa ccgcctatgg ccttctgtgg ctgaggccag acgagcttga gatactgaat
      121 gcgggcaacc cgccgaacaa acggcaagca ttggcgctca ttgccgccgg cacaggattg
      181 ggtgaaggta ttttattctg ggacggcaaa acataccgtc ccatgccgtc ggagggaggc
      241 catacggatt ttgcgcccaa caatgatcaa gaaatcgagc tccttcgcta tctccggact
      301 caatatcttc acgtgagcta tgagcggatt ctctccggtc ccggcctaca tgcgatttat
      361 gagttcttac gcgataccaa gaaaaacgag ccgacgtggc ttgcggaaaa gattcgggct
      421 ggtaatccgg ccgctgaaat ttctcaagcc ggattgcaag gacaagccga gattgctaag
      481 caagcactgg atctcttcac gtcgatttat ggagcagaag ccggcaacct cgccctgaag
      541 gcgctctccc tcgatggggt gtatgtgggc ggcggtatcg cgcccaaact catcacaaag
      601 cttcaagacg gcacgttcat gaaagccttt accaataaag gtcgatataa acggctgatg
      661 agccagatgc cggtgaaagt catcatgaac cagcaaacgg ccttgatcgg tgccgcgtcc
      721 gtcgcagcag ccctttctct tgcttccccg tcatgacgtc aggaaatctc gacagcttca
      781 aaagatccgc tgctctaaag gccgttgaat ttgttcgcga cgggatgatt gtcggactag
      841 ggacaggatc gacggcaaag catctgctca tcgcactcgg cgaacgtgtc cgtgccggca
      901 tgaaactgcg aggcgtgcca acgtcgcaag aaaccgccat gctcgccagg gagtccggta
      961 ttcctctcat cgataccgag aatcggtggg agatcgatgt cgccatcgac ggagccgatc
     1021 aggtcgatcc cgacttcaat ctggtcaaag gtgggggcgg agcgctccta aaagaaaaga
     1081 tcgtggcggc gcgggcgaaa caattcatcg tgatggtgga ctacaccaaa caagtaccgg
     1141 tgctcggagg atccttcccg ctgcctatcg agatcatccc ctttgggtgg ggaagtactg
     1201 cgcgcgaagt tgaagtgctc accaaaagcc gcgtgatact cagagagtgc gatggggtgc
     1261 cgtttaagac cgaggctggt catcttattg tcgacgtcca tatcgaccgc atcgatcggc
     1321 cgggtgaatt ggaaacggca ctgaacctca tccccggtgt agttgagacc ggtctctttg
     1381 tcggccggac gagcgttttg atcgtcggta cgccgcaagg cgttcacact ctccatgtcc
     1441 ccgggaaatg agcgctcgat cagcagacga gaaattctgt tggatttcgg ccgacgcgtc
     1501 tgcttccttc tgactggtcc gatagcgtat acactcgtac catcatccaa atccgccgat
     1561 gccgcaacga ccaccagacc acaaggtgaa gacatgagtc ttgatgatgt tccagacagc
     1621 cacgatctct atcggcttcc gcgacacgtg atcccgacac gctatgacct acggcttgaa
     1681 ccggatctcg ccggcgcaac tttcacaggc caggccacga tcacgatcac ggtcaaacag
     1741 accacccgga tcattctgtt gaatgccgtc gatctcgtgc ttcggtcagc ggtggctgag
     1801 gacgtgaatc agcggcaagt cgcggcgctt atcgaattgg aacaagaggc gcaacgagcc
     1861 cggttttctt ttcaagaacc cgtccagacc ggcgaatggc ggctgcatct ttcctttcat
     1921 ggcaaactga acgatcagct ccgtgggttt tatcgtagta catacaaaga tgcttcagga
     1981 acgccgcaga cgttggcggc cacgcaattc gaagccaccg acgcgcgccg cgcctttccc
     2041 tgttgggatg aaccggattt taaggcggtc ttcgcgacca cgctcgtgat cgacccttat
     2101 ctcacggcag tctcaaacac atcggtgaca tcggagcgcg tcgagaacaa taagaaggta
     2161 atacggtttg ccgacaccat caagatgtcg acctacctcg tggccttcgt ggtcggtcgt
     2221 atggaggcga cggagccggt atgggtcggt aagacacctc ttcggctgtg gacggtccca
     2281 gggaaacaac tgctcacgcc gttcggtcag gatattgcgg tggcatcgtt gaagttcttc
     2341 gaagactact acggcattcc atatcccgga gacaaactgg accttgtggc catcccggac
     2401 tttgcttccg gggcgatgga aaatctcggt gccattacct tccgagagac ggcattactc
     2461 gtggatcgac agactggaac ccatgctgag ctggagcgca tagccgatgt ggtggcccat
     2521 gagaatgcgc acatgtggtt cggcgacttg gtcacgatgg cctggtggaa cggcttatgg
     2581 ttgaacgaag cgttcgcaac cttcatggag atgctggccg tcgacgcgtg gaaaccggaa
     2641 tggaaacgat gggagacctt cggcgtcgcg cgagcggccg cgttctcggt cgatggactg
     2701 gtgagtacca ggccgatcga atatcctgtt cacgcaccaa aagatgccga tgcgatgttc
     2761 gatattttga cctatgaaaa aggcgcgtcg gtccttcgga tgctggagca acatatcggc
     2821 tccacggtct tccgagacgg tgtgcggcag tacctgcgca cccatgccta tggcaatgcc
     2881 gatacgaagg atctgtggac ggcccttggc acggtcgccg gccaaccagt acccgaactg
     2941 atggacggct ggatctttca acccggcttc ccattggtga cggcggaggt gcgtggtcag
     3001 gaactgcttc tctcccagca acgattcacc tacatatccc aagggcagtc tgctgagcag
     3061 ctctggcaga tccccatcca gattcgactg gtcataggcg atcgtacgga acatcgtcgg
     3121 ctgctgtcga cagagcggga gacgactatt ggaataccgg ccggggtcac ctccgtcttc
     3181 gttaacgaag gagggcatgg gttttatcga gtgcgctatc acggtgttct tctgcaacaa
     3241 ctactcgata aggggcttga tcgtctggcg gccatagaac ggttcgccct cgtcagtgat
     3301 gcatgggcta caacggtggc cgggctcacg gcacttccgg cgtacctccg actcaccgcc
     3361 catttcaagc gtgagcggga caagaacgtc tgggcggtgt tgctggactc gttctccttc
     3421 ttgagcagga tcatctcctc agaagatcgg ggagcactcg aaacctttgt gcgcgcctgc
     3481 gtaaagccgg cgatagatga attgggatgg gatgcgaagc ccggagaatc ggatctcgtt
     3541 cggcaattgc gcggcgatct gcttggcgcc ctcgggagac tcggcaatga tgctgcgacg
     3601 cagcaggaag cagcgaaccg ctatcgacga tatcgcaagg acccgacgac catcgatcag
     3661 aatgtcgtgc ccgctctcgt ggccattctg gcccatacag gagacgaggc acgttacgat
     3721 gaatttttag agctctaccg aacggcccct actcctcagg aagaacggcg gtacctgttc
     3781 tccttggcct catttcgaca cgaggaatta ctcacgcgca cgctgtcccg caccatcaac
     3841 ggcgagatcc gcacccaaga tgcccccttc atcgtcggag cgctgttgac gaatgtgtat
     3901 ggccgggaac tcgcctggga ttttgtgaaa gccaattggc atcacatgga ccggctgttc
     3961 ccgaaacaag gccttcgccg tatgtgcggg ggaatcgtcg gtctcgtcac tccggaactc
     4021 gaacaggatg tgcgccagtt ctttacctcc cggaagatcg acctgggtgg gaaaactttg
     4081 gatcaatacc tcgaacaact tcgtgttgcc gtggcattcc atgagcgaga aggacggacg
     4141 atcaaagctg ccctcctcag ttctttgcag gcatgaaatc cagggcatct ctttctcagc
     4201 tgattcgtgg atgctcgggg taggatgcag aaagaaacga tctggaaata ccctccatac
     4261 gtac
//
LOCUS       DATMTI010000588         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_573295, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000588 DATMTI010000000
VERSION     DATMTI010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573295"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VN039_09365"
     CDS             complement(<1..219)
                     /locus_tag="VN039_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46210.1"
                     /translation="MERRAASHTEEEEMNQRRKLLNAARRGDTKAISKLFELYQVRVL
                     SGDQLAKVNRTFTYMAPVKSQSSKSKAAE"
     gene            475..1146
                     /locus_tag="VN039_09370"
     CDS             475..1146
                     /locus_tag="VN039_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HWV46211.1"
                     /translation="MEHTQESSTVTEDPQARDLLRRAFEGTARWQKDFKGFTADLTVN
                     VNGKEVSGSVMVKGPREVSVQLGDGETQKWVQEQLGMIAVHRGPRSFEESDGKYSLTK
                     EEDGHPFGTKLNIHGSNSFYRIKDNRITQINRKMAHPGMNPFAFTINVEESALTQDRK
                     NLTTKYTVYYYSPTDGKLTNVESFTDTHVRVGSSDLPATRRIITYEDGQVVVKNLTFK
                     NHKLL"
     gene            1149..1598
                     /locus_tag="VN039_09375"
     CDS             1149..1598
                     /locus_tag="VN039_09375"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HWV46212.1"
                     /translation="MDLRKDFPRSMRFKLAGYAHLARMIDKCRAVLAGTEGEYIYPCP
                     MDERLMEFVGITSDQFTSVVKANSTDEGVVKWLEQTATPHQPAELEEWNQKLLVRGPS
                     SPESAVKFKQYLAAIDPSRTDITAWSDLQDLEEGRSVPRREPAHHCQ"
     gene            complement(1592..2218)
                     /locus_tag="VN039_09380"
     CDS             complement(1592..2218)
                     /locus_tag="VN039_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWV46213.1"
                     /translation="MKITVIGASAGVGLQATRLALEKGHEVATLSRRVVPLPDHTKLK
                     RMQGSATSVNDVRVAVEGAGAILVTLGVKSPFATTLFSDSARILLRVLQDNGSSATLI
                     VLTGFGTGDSWSYNSFPMKLLFTLLLKAVYADKSEQERIIAGAYSRWEIVRPGRLING
                     TRTSHYRVLDDLVEGMRVGAISRSDVAHFMVAQAENPTYLGKYVALTY"
     gene            complement(2334..3065)
                     /locus_tag="VN039_09385"
     CDS             complement(2334..3065)
                     /locus_tag="VN039_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV46214.1"
                     /translation="MAKITEIAPDLFRITTFVEPFNIQFSQFLMRDDEPLLFHTGPRV
                     LFPEVRAAVASLIDPKNLRWIGFSHFESDECATVPEWQQLAPHSEAVCSVVGKMVSVD
                     DCLALRPAKGMVDGEVLETGRYRFRFMATPHVPHCWEAGLMFEETERTLLCSDLFHQN
                     GDVEPMTQSDVIDRCRKTLVDYQQGPLANYVPYCSLTEATLHRLAALQPRRLATMHGS
                     VYIGDGSKALHDLAVVWQEILRPPS"
BASE COUNT          805 a          914 c          878 g          718 t
ORIGIN      
        1 ctccgcagcc tttgatttac tgctctgtga cttcacggga gccatataag taaaggtgcg
       61 gttgaccttg gccaattggt ctccgctgag gacacgtact tgatagagct cgaacagctt
      121 gctgatggct ttcgtgtcgc ctcgtctcgc cgcattcaag agtttgcggc gttgattcat
      181 ctcttcttct tcggtatggg aagcagctcg tcgttccatg cccatccttc ctcaacacag
      241 gctgaccggc tgatcaggct gctgcggtcc aacaccccag cctaaaaaaa tcgccgaagt
      301 atatcagaat tatgatgatt tttgaaatat cgaatgttgc gcgaccgtgc gatgcatcct
      361 ctcatgatgt cgaaacgcaa aaggccgcag cccaagggat ttcactattg agtgcgatcg
      421 cacgcaccgg tataagtata gaaggtcgat acgtttttta agaggagatc catcatggag
      481 catacgcaag aatcttcaac cgttaccgaa gaccctcaag cgcgcgatct actgcgccga
      541 gcattcgaag ggaccgcacg gtggcaaaaa gactttaagg ggtttacggc cgacctcact
      601 gtgaatgtga acgggaaaga agtcagcggg tccgtcatgg tcaaaggccc ccgcgaagta
      661 tccgttcaac taggggatgg cgagacccaa aaatgggtgc aagagcaact aggcatgatc
      721 gcggtccatc gcgggccgcg gagcttcgag gagtcggatg gcaagtactc gctcacgaag
      781 gaggaagacg gccatccatt cggcaccaag ctgaacatcc atggctccaa ttccttctat
      841 cgcatcaaag acaatcgcat cacacagatc aatcgcaaaa tggcgcatcc gggcatgaat
      901 ccgttcgcct ttacgatcaa cgtcgaggag agcgcgctca cacaggaccg gaaaaatctc
      961 acgaccaagt acacagtcta ttactattcg ccgaccgacg gtaaactgac caacgtagaa
     1021 agtttcaccg acacccatgt ccgcgtcggc tcgtccgacc ttccggcaac caggcggatc
     1081 atcacctatg aagacggcca ggtcgttgta aagaacctga cctttaagaa ccacaagttg
     1141 ctctaacgat ggatttgcgc aaagacttcc ctcgcagcat gcgattcaag ctggcaggct
     1201 atgcccacct cgcccgcatg atcgacaagt gccgggctgt gctcgcaggg acagaggggg
     1261 aatatatcta tccttgtcca atggacgaac ggctgatgga gtttgttgga attaccagcg
     1321 atcagttcac atccgtagtg aaggccaatt cgaccgatga gggagtcgtg aaatggctgg
     1381 agcaaacggc aacaccgcac cagcctgccg aattggagga atggaatcaa aagctgttgg
     1441 tacgcggacc gagttctcct gagagtgcag tcaagttcaa acaatacttg gccgccatcg
     1501 atccatcccg caccgatatt acagcttggt cggaccttca agatctggaa gaaggacggt
     1561 ccgttccgag acgagaacca gcacatcact gtcaataagt gagcgctacg tacttgccga
     1621 ggtaggtggg attctctgcc tgagccacca taaagtgcgc cacatcagaa cgggaaatag
     1681 cgcctacccg catcccttcc accagatcgt ccaagacacg ataatggctc gtccttgtgc
     1741 cgttgattag ccggcccggg cgtacgattt cccagcggga atacgctcca gcaattatgc
     1801 gctcctgctc acttttgtct gcatagactg ccttgaggag tagcgtgaac aaaagcttca
     1861 taggaaacga gttgtagctc cagctgtcgc ccgtaccgaa tccggtgagc acgatgagtg
     1921 ttgccgagga accgttgtct tgtagcacgc gcagcaagat gcgtgcagag tcggaaaata
     1981 atgtagtggc aaagggactc ttcacaccca gcgtgaccag gatggcgcca gccccttcca
     2041 ctgcgacccg cacatcattt acgcttgtcg cactcccctg cattcttttt aatttagtgt
     2101 gatccggaag tggaacgacc cgcctcgata aggtggcgac ctcatgcccc ttctctaaag
     2161 cgaggcgtgt ggcctggagt ccgaccccgg ctgaggctcc gataacagtg attttcatag
     2221 ttcctgtgct tgttgcagac aatctaaaaa catatagatg gagcagacac attctcagct
     2281 tgtttatgga ctcgggtcaa gcgccgttga tccctattcc tcagtccttg ggatcatgac
     2341 ggcggcctga ggatctcctg ccaaacaacg gcgagatcgt gaagcgcttt gctcccatca
     2401 ccgatgtaga ccgagccatg catggtagcc agccgccttg gctgtagcgc tgccagccgg
     2461 tgcagcgtcg cttccgttaa cgagcaatag ggcacgtaat ttgccaacgg tccttgctga
     2521 tagtccacca gcgtcttccg acagcgatcg atcacgtccg attgcgtcat cggttcaacg
     2581 tccccgtttt ggtgaaagag atccgagcac aggagcgtcc gctccgtttc ttcgaacatc
     2641 aatcccgctt cccagcagtg tgggacatgc ggcgtggcca taaatcggaa ccggtatcgt
     2701 ccggtctcaa gcacctcgcc gtcgaccatc cccttggcgg gacgaagcgc cagacaatcg
     2761 tcgacgctca ccatcttgcc caccacgctg cacactgctt ccgaatgcgg ggctagttgc
     2821 tgccactccg gcactgtcgc gcattcgtcg gattcaaaat ggctgaatcc gatccagcgt
     2881 aagttcttcg gatcgatgag cgacgccacg gcggctctga cctcgggaaa cagtactcgc
     2941 gggccggtat gaaacagcag cggctcatcg tcgcgcatca agaactggct gaactgaata
     3001 ttgaacggtt caacgaaggt cgtgattcga aataggtccg gggcaatctc ggtgatcttc
     3061 gccatgaaac ctcctcaaag aatggagccg tgcgaatcat acgcaccgcc ggaccgaggt
     3121 gcaataagat ggatgaggat gatgacagaa gagctgtggc tatgaactca ctgagactgt
     3181 gccatggaac gatcctgaaa acatgaagtg gactgactgt gcggcggatg gatgtctcaa
     3241 aagattcccg agcctctatt aggccccatt gtgttcgatt ttgccgggaa gcttgctgag
     3301 aatcagtttt tgtag
//
LOCUS       DATMTI010000589         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_700243, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000589 DATMTI010000000
VERSION     DATMTI010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700243"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..>1864
                     /locus_tag="VN039_09390"
     CDS             227..>1864
                     /locus_tag="VN039_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HWV46215.1"
                     /translation="MKVRLNPRQSAPAFAVMMALSITTVLAYPGIARAADTLAPADDV
                     QASDETSPTPVSEFAPDGVRAAHGQGYDQGLTINRVSIEGNKLINSDKIKDTMVIRPG
                     SLYSKSTLKEDLRRIYDMGYFTEKIKAVPVATSDGIVLRIVVQENAPVTGVHIDGNTI
                     VDDNELQSIFASQTGMPQNIGQLNEAIEKVEKLYAEKGYVLARVANIEDDPDGVINLK
                     INEGTIDKIQFVGNRKTKDYVLKRMMSMKSGDVYNEKKLSEDMKRLFGTQAFKDVRRV
                     ITASPDDPDKYNLTVEVDEKRTGAISLGGGIDTTTGLFGSAGYNDPNFLGRGQNFSSV
                     FAVGSGIINRNAATQANTRTYQFDVGWSNPSLMNTANSLSVNAYGRNYSSFNVPLGVE
                     RRFGSEVTWGTPIKNLNHTSFALSLRGEHVGLLDYSTSDELAKFNITSSQRAAQLKGG
                     NFLTLSPTIAYDTRDNRFSPTSGWLNTISIGGSYGLGGSTSYGMANANLRKYIKIRDG
                     ITLALNAQGGHSIMGDIPAFNMYRLGGSYSVRGFQQGG"
BASE COUNT          449 a          558 c          490 g          367 t
ORIGIN      
        1 atgttgagag ggcacatttt tggttctgcc tcgtcctcaa caaaatccga cagtaaaacg
       61 tggcaaaacc aagtgctaag ttccctgaag ccccctgaag cccaagagag cccccaagcc
      121 cttgtttaat aagttcttta cgcctactag accctatggg acaatcctag taagtgctga
      181 gatgatctaa gataaccaga cgcctgtaac agatgaggtt agcctgttga aagttcgttt
      241 aaatccccgt cagtccgccc ccgcatttgc cgttatgatg gctttgtcca tcacgacggt
      301 cttggcgtac cccgggattg cccgcgcagc tgataccctg gccccggctg acgatgtcca
      361 ggccagcgat gagacttctc ccaccccggt cagcgaattc gccccggacg gtgtgcgcgc
      421 cgcccacggc caaggttacg accagggtct caccatcaac cgggtttcca ttgagggcaa
      481 caagctcatc aacagcgaca agatcaagga caccatggtc atccggcccg gcagcctcta
      541 cagtaagagc acgctaaaag aggatctgcg ccggatttac gacatgggtt acttcacgga
      601 aaagattaaa gccgtgccgg tcgccaccag cgacggcatc gtgctgcgta tcgtcgtgca
      661 ggagaacgcc ccggtaaccg gcgtccacat cgatggcaac accattgtgg atgataacga
      721 gctgcaatcc atcttcgcca gccagactgg gatgccccag aatatcggcc agctcaacga
      781 agccattgaa aaagtcgaga agctctacgc cgagaaaggc tatgtgctgg cgcgggttgc
      841 gaacatcgag gatgacccgg acggcgtgat caacctgaaa atcaacgaag gcacgatcga
      901 caagatccag ttcgtgggca accgaaagac caaggattac gtcctgaagc ggatgatgtc
      961 catgaagtcg ggcgatgtct acaacgagaa aaagctctcc gaggacatga agcggctctt
     1021 cggcacccag gcgttcaagg acgttcgccg cgtgattacc gcttccccgg atgacccgga
     1081 caagtacaat ctgaccgtgg aagttgatga gaaacgcacc ggcgccatct cgctaggcgg
     1141 cggtatcgat accaccactg gtctgttcgg ctcggcgggc tacaacgatc ccaacttcct
     1201 gggccggggc cagaacttca gttccgtgtt tgcggtgggt agcgggatca tcaaccggaa
     1261 cgccgctacc caggccaaca ccagaactta tcagttcgat gtcggctgga gtaaccccag
     1321 cttgatgaac acggccaact cacttagcgt gaacgcatat ggccgcaact acagcagctt
     1381 taacgtccca ctgggcgtag aaagacgctt tggcagcgag gttacttggg gcaccccgat
     1441 caagaacctg aaccacacct catttgccct cagcctgaga ggagagcacg ttggtttgct
     1501 tgattactca acctccgacg aactcgccaa gtttaacatt acctcctccc agcgtgcggc
     1561 ccagctgaag ggcggcaact tcctgactct atcccccaca atcgcctatg acacccggga
     1621 taatcgtttc agcccgacct ccgggtggtt aaacaccata tccatcggcg gttcctatgg
     1681 tttgggcggc agcacctcct acgggatggc caacgccaac ctgcgtaaat acatcaaaat
     1741 ccgtgatggc attacgctcg ccttgaacgc tcagggcggc cactccatta tgggcgacat
     1801 cccggccttt aacatgtacc ggttgggcgg ctcctactca gtgcggggct tccagcaagg
     1861 cggt
//
LOCUS       DATMTI010000590         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_362052, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000590 DATMTI010000000
VERSION     DATMTI010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362052"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..>2525)
                     /locus_tag="VN039_09395"
     CDS             complement(33..>2525)
                     /locus_tag="VN039_09395"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HWV46216.1"
                     /translation="MRQYLAAAFLIVIGLVASGCGDTATVNSAATLANLTVDPGTLQP
                     AFSGGVTQYTVDLSNDVTSVTIAAQPSVAGDTVTINNQVTTSSVITLGAAGTTTSVPI
                     TVSGSAASPQTYTVNLVRASFSNSLQSLVVSPGTLAPTFNMNQLTYSVDVANNVGSIS
                     VTPTVQDPAATVTVNGQPAISGQASTVPLNNPGQSQTTVIPIVVKAQNENEKTYTVTV
                     NRGVSSNKNLSGLTVSPGTLNPTFNANRTSYTVNVASTVTSVTVTPTLQDTTASMTVN
                     GQTNSGQARTIQLSGAGSSTPIIIIVTAQNGTQNVYTVGINRAALGGNNNLSALAVSP
                     GTLDPAFNANTTNYTVDVASTVTSATVTPRLQDTTASMTVNGQATNSGQARTIQLGTP
                     GSSPPPILIVVTAQNGTQKTYTVDVNRDALGGNNNLSGLAVSPGSLSPAFTATRTSYT
                     VSVGSGVDSITVTATKADSNASMTINNQGTNSGQSRPINLGAPGSSTEIDITVTAPNG
                     NSKAYTITVERAAPSSNNNLSALRVTSGNVAQTLTPQFSAGTLGYTVNVATNVTSVTV
                     TATKADSNAVISGDLPTQGQATIQLDGPGISKTVSIIVTAPSGAQKTYTVTINRATPS
                     SNNNLSALSVTPGALSPAFAADTLGYTVNVASDVTSVAISATKADSNAVISGDVPNSG
                     QANIALSGAGTTTSVSIIVTAPDGTSKTYTIDIIRAAPSAPPAPASAPDLIPQDDSGF
                     LPGQDADNITNVNTPRFRVPSPGAGETPNLYVDGSKVDATFDQGANTLRPTTALSDGD
                     HTITSTVTNAGGESSPSPPLPVTIDTIAPGLP"
BASE COUNT          459 a          642 c          778 g          646 t
ORIGIN      
        1 cggcggcaaa gcccgcctct attgtcctgg tgctacggga gtccgggggc tatcgtgtca
       61 atggtcacag gcaagggtgg actcggagaa gactcacccc cggcattggt caccgtactg
      121 gtaatcgtat ggtcaccgtc gctcaacgct gtcgtcggcc taagggtatt ggctccttga
      181 tcgaaggtcg catcgacttt gcttccatct acatagaggt tcggcgtttc tccagccccc
      241 ggcgatggca ccctaaatct tggtgtgttg acattggtga tgttgtccgc atcctggccg
      301 gggaggaatc cggagtcatc ctgcgggatc aggtctggtg cgctcgccgg tgccggtggt
      361 gctgagggcg ctgctctgat gatgtcgatg gtataggtct tagaagttcc atcgggcgca
      421 gtgacgataa tcgataccga tgtcgtggtc cctgctccgg agagcgcaat attcgcctga
      481 ccgctgttag gcacatcccc agagatcacc gcattcgagt ccgccttggt cgcggagatg
      541 gcaacgctcg tgacgtcgct cgctacgttt acggtgtatc ccagtgtgtc tgcggcaaag
      601 gcaggggaca aggcgcccgg tgtcacgctc aatgctgaca gattgttgtt gctcgatggt
      661 gtcgcgcggt taatggtgac ggtgtaggtc ttttgggctc cggaaggtgc cgtgacaatg
      721 atcgacacgg ttttactgat ccctggaccg tcaagttgga tggttgcttg gccttgagtg
      781 ggcagatcac ccgatatcac cgcgtttgaa tccgccttgg tggcggtgac ggtcacgctg
      841 gtgacgttgg ttgctacgtt gaccgtgtaa cctaacgttc ctgccgaaaa ttgcggagtt
      901 aaggtctgag ctacgttgcc ggatgtcacc ctcaacgccg acagattatt gttgcttgag
      961 ggtgccgcgc gctctacggt gatggtgtaa gctttggagt taccattcgg ggcagtcaca
     1021 gtaatgtcga tttccgtgct cgatcccgga gcgccgagat tgatggggcg cgattggccg
     1081 gagttggtcc cctggttatt gatcgtcatg ctggcattcg agtccgcctt ggtcgcggtt
     1141 acggtgatac tgtcgacgcc gctgccgacg cttaccgtgt aacttgttct agtcgcagta
     1201 aaggcggggg acagggaacc cggcgatacg gccaatccag agagattatt gtttccgcca
     1261 agggcgtcac gattgacatc cactgtataa gttttctggg tgccgttctg cgccgtcaca
     1321 acaataagga taggtggtgg gctcgaaccc ggtgtgccca attgaatggt tcgagcttgg
     1381 ccggagttgg tggcctgccc attcacggtc atgctcgctg tagtatcttg tagcctcggc
     1441 gtgactgtgg cgctcgtcac ggtgcttgcc acgtctaccg tatagttcgt cgtgtttgca
     1501 ttaaaagcgg ggtctagggt gcccggtgac acggccaatg cagaaagatt gttgttccca
     1561 ccgagtgcgg cacgattaat acccaccgta tagacgttct gggtgccgtt ctgtgccgtc
     1621 acaataataa taataggggt gctcgaaccc gctccgctca attgaatggt tcgagcctgg
     1681 ccggagttgg tttgtccatt caccgtcatg ctcgccgtgg tatcttgcag cgtcggtgtc
     1741 accgtgacgc tggtcactgt gctcgccaca ttcaccgtat agctcgtcct gtttgcatta
     1801 aaggtcgggt tcaaggtccc cggtgatacg gtcaagcctg acaagttctt gttgctggat
     1861 actccacggt ttacggtgac tgtgtacgtc ttctcatttt cattctgagc cttcacgacg
     1921 atcgggataa cagtggtttg actttgaccg gggttattga gggggactgt actggcctgc
     1981 ccggagatgg ccggttgccc gtttaccgtt accgtcgcag cggggtcttg cactgtgggg
     2041 gttacgctga tacttccgac attgttggcg acatcgactg aataggtgag ctgattcatg
     2101 ttgaacgttg gggccagcgt tcctggtgaa acgactaaac tctgtaacga attgctgaag
     2161 ctggctctga caagattgac cgtgtaggtt tgcggacttg cggctgatcc tgacactgtg
     2221 atgggcacag aggtcgtcgt tcccgccgcc cccagggtaa taacactgct cgtggtgacc
     2281 tgattattga tcgttaccgt atctcccgcc acggagggtt gagcggctat tgtcacgctc
     2341 gtaacgtcgt tggaaagatc gacggtgtac tgagtgactc ccccactgaa ggctggttga
     2401 agagttccgg gatcgaccgt taggttggcg agtgttgccg cggagttaac tgttgccgtg
     2461 tcgccacagc cggaggctac caagccgatc acgatgagaa aagcggcagc gagatactgt
     2521 ctcat
//
LOCUS       DATMTI010000591         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_615879, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000591 DATMTI010000000
VERSION     DATMTI010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615879"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..672)
                     /locus_tag="VN039_09400"
     CDS             complement(28..672)
                     /locus_tag="VN039_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46217.1"
                     /translation="MKASSFLLSILAALVTGCALSQEVSRTPRTAIEQVLLTQAVEQA
                     LANLSVRLPDGVNVDVDVTGLESDRSRLGMTNADLGTINVPSRDILYIRDAVAAELGR
                     QGYRVSARGAESPYLVRVMAESFGTMQAIVFVGMPPVQSVLIPFSLPELTLYRNQSQS
                     GYVRLHVDIYDNRTGEFLGSTPKLVGRAYFNQYTVLLLATWHRTDVTAYPVAGR"
     gene            complement(896..>2149)
                     /locus_tag="VN039_09405"
     CDS             complement(896..>2149)
                     /locus_tag="VN039_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWV46218.1"
                     /translation="TDELHGEMNQLKALVDLAHLHGLAMILDVVYNHAGGEFGDQSLY
                     FFDRQPQAGGNNNSLYFTDKGHAGGLVFDYAKPEVRDFLIQNAKFFLDEYRIDGFRYD
                     QVSVIDHEGAPHGWRFCQDLTSTLHADRPSALHHAEYWSVNPVVVQPVPEGAGFDTSL
                     TDGLRIILRNVIGNASAPDDRPLDMTGLAHSLWPEGFSEQWRFVQGPENHDIVYRDRE
                     LRLARLADPSNPRSWYGRSRTRVATDLSLTAPGIPMLFMGQEFLEDKQWADDFISHPE
                     LLLYWTGLEQGDKQMADHVRFTRELLACRRNSSGLRGQGFRVVHVHDQNRVLAFHRWV
                     EGQGHDVIVVAHLAPFHRFGYRIGFPSGGDWREAFNSDVYENWVNPGVVGNGGQVIAD
                     PYPMHDLAFSAALVLPANSILVFAR"
BASE COUNT          487 a          620 c          609 g          433 t
ORIGIN      
        1 cggtgcggaa cggaaatatt tactccctca tcttcccgct acgggatagg ccgtcacatc
       61 ggtgcggtgc caggtcgcaa gaagcagcac cgtgtactga ttgaaatatg ctcgcccgac
      121 cagtttggga gtcgaaccga gaaactcgcc ggtccgatta tcgtagatat ccacatgcag
      181 ccgaacatag ccgctctgac tttgattcct gtagagagtg agttcgggaa gcgagaacgg
      241 aatcaacacg ctctgaaccg gcggcatccc gacgaatacg atagcttgca tcgttccgaa
      301 cgactccgcc ataacccgaa ccaagtacgg ggactcggcg cctcgtgcgg agacgcgata
      361 gccttggcgt cccaattcgg cggccacggc gtctcggatg tacaaaatat cgcgcgatgg
      421 aacattgatc gtgcctaaat ccgcgttcgt cataccgagc cgagaccgat cactttcgag
      481 cccggtcaca tccacatcca cattgacgcc gtcgggtaac cgaaccgaca gattagccaa
      541 ggcttgctcg accgcctgcg tcaataggac ctgctcgatc gccgttcgag gagtgcgtga
      601 tacttcctgt gaaagtgcgc atcccgtgac caacgcagcc aggatagaga ggagaaaaga
      661 ggaagccttc atgagagtcc tgacaaccag ttcagtccca taaaagaatg ctagtcgcgt
      721 tcaacgagga attcaaccta ggccgatgcc gatctagaac gtggcctgag gactgcacct
      781 cagccggcag ccgaagtggt gcaaatactt cttctttacg aaggactcca tcgcagggct
      841 tctgtggttc tcaggatctt gtgtactgag ggacgagaca ctatcgagcg caaagttacc
      901 gcgcaaacac gagtatgctg ttggccggaa ggaccagcgc agctgaaaag gcaaggtcgt
      961 gcatggggta tggatccgcg atcacttgtc cgccgttgcc gaccacgccc ggattcaccc
     1021 agttctcgta tacgtcgctg ttgaaggctt cccgccagtc tccgccgctt gggaatccga
     1081 tccgatatcc aaagcgatga aacggggcca ggtgggctac cactatcacg tcgtgtccct
     1141 gtccctccac ccaccggtgg aatgcgagta cccgattctg atcgtgcacg tgcacgacgc
     1201 gaaatccttg cccccgaagc ccggatgagt tccgacgaca ggcaagcaac tctctggtga
     1261 agcgcacatg gtcggccatc tgcttgtcac cctgttccag cccggtccag tagagaagca
     1321 gctccgggtg agagatgaaa tcatctgccc actgcttgtc ctccaagaac tcctgtccca
     1381 tgaacagcat cggaattcct ggagccgtca agctgaggtc ggtggcgacg cgggtacggc
     1441 ttcgtccata ccaggaacga ggattggagg ggtcggcgag gcgagcgagg cggagttccc
     1501 ggtcacgata cacgatgtca tgattttccg gaccctgcac gaaccgccac tgttctgaaa
     1561 agccctcggg ccagaggcta tgagcgaggc ccgtcatgtc gagcggacgg tcatccgggg
     1621 cgctggcatt tccaatcacg ttgcgcagga tgatgcgtaa cccgtcggtg agggacgtat
     1681 cgaatcctgc tccttccggt accggctgta ccacaacggg atttacactc caatactcgg
     1741 cgtgatgtaa ggctgaaggt cgatctgcgt gcagcgtgga agtgaggtcc tgacagaatc
     1801 gccagccatg cggggcaccc tcgtggtcaa tgacgctcac ctgatcgtag cggaagccgt
     1861 cgatgcgata ttcgtcgagg aaaaatttgg cgttctgaat gaggaagtcg cggacctccg
     1921 gtttggcata atcgaacaca agcccgcccg catgaccctt gtctgtgaaa tagagggagt
     1981 tgttgttccc gcccgcttgg ggctgtcgat cgaagaagta gagactctgg tctccgaatt
     2041 cccctccggc atggttatag accacgtcga gaatcatggc caacccatga aggtgtgcga
     2101 gatccaccag cgccttcagc tggttcatct ccccatgcaa ctcatcggt
//
LOCUS       DATMTI010000592         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_786360, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000592 DATMTI010000000
VERSION     DATMTI010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786360"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..292)
                     /locus_tag="VN039_09410"
     CDS             complement(155..292)
                     /locus_tag="VN039_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46219.1"
                     /translation="MSECVVKRHGDVVDGPMDVWEARDRAIELNERYQTDEYRVEEFD
                     A"
     gene            complement(289..642)
                     /locus_tag="VN039_09415"
     CDS             complement(289..642)
                     /locus_tag="VN039_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46220.1"
                     /translation="MNYSADDLLALIDRHDGHETFYRERLAEDYPLVASLAAKLEGKP
                     LDRGEWERRVLDEGERQVALGYTPEHDREHGPDHLILEGLERAFRGDTVQVGAMLLAL
                     RRLLADTDEPKGDEQ"
     gene            complement(639..1283)
                     /locus_tag="VN039_09420"
     CDS             complement(639..1283)
                     /locus_tag="VN039_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46221.1"
                     /translation="MVKNYIAIVFDGPPSHEAGRFIEVNDSHGKSIDIGTWVSRGDGS
                     WELRIAVDKLKDDNDMTIDTSREAVQALIAEALDYAGEDQCGGAELLRETAQTLGETA
                     QTLTAVLDERDEALRSNKNLRHISNANGDWSEKYQAALRRAECAEAGYETMLESLNRV
                     ESENAALTAKLDAVKKYADDRAFHARGHLNTVNSGRIADDLRAILADTDEGNEP"
     gene            complement(1283..>2864)
                     /locus_tag="VN039_09425"
     CDS             complement(1283..>2864)
                     /locus_tag="VN039_09425"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWV46222.1"
                     /translation="AARRWILGDEMGLGKTRTSIAWLDLVDAHKVIVVTPAGICNQFA
                     GELEQYAPHREVVNLYKRLPVVRHELLDNMLSKDEGVIVVNYEIWRRDKDVLAKLIRW
                     QADTLLVDEAHNIKTTSTANYKAISMLVEGQNTCPNCGAWVKGLIEAKTYRKIPCETC
                     GWRQGDPLDDEYLNPLEEWLASRSILNVCFTTGTPLLNSPEDIYSLLHLCDPKMFPTK
                     ANFLSTYCSNNHFSGKWEFLPVALKLLKPLIADRFLARNKEDAGVVVPTQHVHVIPIA
                     IDRQEYPKQYRLIRQISEAAQIILESGEKASLMHLISILTRKRQANVWPGGIKIEAPD
                     GRVLVDVGNEVSESCKMDFIQDHIELLAEQGHRQIVFSQFKTALAEFEQRLSKAGMRV
                     ARYDGDTPAKLRTEIEANFYKAKGENAKWDVLLANYKTGGTGLNLTSATKIHVLDEEW
                     NPGKRDQAYHRVARMGQDQETDVYVYRLTGTVDTWVANTIARKERMVDTFNETFAEDA
                     PELTVENLLEALRNGDML"
BASE COUNT          570 a          850 c          710 g          734 t
ORIGIN      
        1 ctcccatgcg ccctcgcaca cgagcccgct ttcgttgttg cggccacgcg cggcatcggc
       61 tcgcacccag gattcatatc cgtgctcgta ctgccacggt ccttcggttg cggcgcccgt
      121 cttggccttc cagtcggcca acagtgcttc ggtgtcaggc atcgaactcc tccactcggt
      181 actcgtcggt ctgataccgc tcgttcagtt cgatggctcg gtcgcgtgct tcccacacat
      241 ccatcgggcc atcgacaaca tcgccatgcc gcttcacaac gcactcgctc actgctcgtc
      301 tcccttcggt tcgtcggtat cggccagcaa tcggcgaagc gccaggagca tcgcaccaac
      361 ctgcacggtg tcacccctga acgcacgttc caggccttcc agaatcaggt gatccggtcc
      421 gtgctcgcgg tcatgctcag gggtgtagcc gagcgctacc tgccgctcgc cctcgtccag
      481 aacgcgccgc tcccactcgc cccgatccag cggcttccct tcgagcttgg cggcgagaga
      541 cgccacgagt gggtagtcct cggccagccg ttcccggtag aaagtctcgt gcccgtcatg
      601 ccggtcaatg agtgccagca ggtcgtctgc gctgtagttc atggttcgtt cccttcgtcg
      661 gtatcggcca ggatggcgcg gaggtcgtct gcgatccgcc ccgagttgac cgtgttcaag
      721 tggccccgtg catggaatgc acgatcatcg gcatacttct tcacggcgtc gagcttcgcg
      781 gtgagagcgg cgttctccga ctcgacccgg ttcagagatt cgagcatcgt ctcgtagcca
      841 gcttcagcac actcggcacg cctgagagcc gcttggtact tctccgacca gtcaccgttc
      901 gcgttggaga tgtgtcgcag attcttgttc gaacgcaacg cttcgtcccg ctcgtccagt
      961 acggcggtga gagtctgggc ggtttcacca agagtctggg cggtttcacg tagcaactcc
     1021 gcgccgccac actgatcttc accggcatag tcaagggctt ctgcgatcag ggcttgtaca
     1081 gcctcacggc tcgtatcaat ggtcatgtcg ttgtcatcct taagcttgtc tactgctatt
     1141 ctcagctccc acgatccgtc gccacgagat acccacgtac caatgtcaat ggacttaccg
     1201 tgtgaatcat ttacttctat aaatctacca gcttcatggc taggaggccc gtcgaaaaca
     1261 attgcgatgt agttcttaac cattataaca tgtctccgtt tcttaaagct tcaagcaagt
     1321 tctcgacagt gagttcaggt gcgtcctcag caaatgtctc attgaacgtg tcaaccattc
     1381 gctctttacg agcgatagtg tttgccaccc aagtgtcaac tgtgccagtc agcctgtaaa
     1441 cgtacacgtc tgtttcttgg tcctgaccca tgcgcgctac tcggtggtaa gcttggtcac
     1501 gcttgccggg gttccactct tcatctagta cgtgaatctt agtcgcacta gtgaggttta
     1561 gtccagtgcc gccagtcttg tagtttgcaa gcaacacgtc ccacttggca ttctcacctt
     1621 tagccttata gaagttagct tcaatctcag tacgcagctt ggctggcgtg tctccatcat
     1681 agcgagctac tcgcatccca gccttgctaa gtcgctgctc aaactctgca agagccgtct
     1741 tgaactggct gaatacaatc tgtcggtgcc cttgttcagc cagcaactca atgtgatctt
     1801 gtatgaagtc catcttgcag ctttcgctta cttcgttgcc tacatctact agcactctac
     1861 catcaggcgc ttcaatcttg atacctccag gccacacatt agcttgacgc ttacgtgtca
     1921 agatcgagat taggtgcatc aagctggcct tttcacctga ctccaaaatg atttgagctg
     1981 cttcgctgat ctgccgaata agtctgtact gcttcggata ctcctgccgg tcaatcgcaa
     2041 tggggataac gtgtacgtgc tgtgtaggta ctacaactcc agcatcttcc ttgtttcgtg
     2101 caaggaatcg gtcagcaata agcggcttca gtagcttaag cgcgacaggt agaaactccc
     2161 acttgccact gaagtgattg ttagagcaat acgtggacag gaagttagct ttggttggaa
     2221 acatcttcgg gtcgcacaaa tgcaacagcg agtagatatc ttctggactg ttgagcagcg
     2281 gtgtccctgt cgtaaaacac acgttgagga tgctacgact agccaaccac tcttcaaggg
     2341 ggttcaggta ctcatcgtct agcgggtcac cctgtcgcca cccacatgtt tcacagggaa
     2401 tttttcgata agtcttagct tcaataagcc cttttaccca agctccgcaa ttagggcaag
     2461 tgttctgacc ctctacaagc attgaaatag ccttgtaatt agctgtgcta gttgttttga
     2521 tgttgtgtgc ctcatctaca agcaacgtgt ctgcctgcca gcggataagc tttgccagca
     2581 cgtccttatc tctacgccag atttcgtagt tgacgacaat cacgccttca tcttttgaca
     2641 acatgttgtc aagtagctcg tggcgcacga caggtaggcg cttgtagagg ttcactactt
     2701 cacggtgcgg tgcgtactgt tccagctccc cggcaaactg attgcagata ccggcaggcg
     2761 tgaccacgat caccttgtga gcgtctacga ggtcgagcca ggctattgag gtgcgagtct
     2821 tacctagccc catttcatcg cccagaatcc agcgtcgggc cgct
//
LOCUS       DATMTI010000593         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_489405, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000593 DATMTI010000000
VERSION     DATMTI010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489405"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VN039_09430"
     CDS             complement(<1..826)
                     /locus_tag="VN039_09430"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWV46223.1"
                     /translation="MNNVVRVKDQYYILASSSLADGRTEVLKQGETFAVFDRYGDMHQ
                     ALPGPQGLYHEGTRFLSRFELSLGAQRPMLLSAMVKEDNALVRIDLTNPDLMDGQRPV
                     PRGTLHLCRTRFLWQGTCHERIQVHNYGDRTFPLLLTMTMDTDFADLFEARGRQRPRR
                     GWRLNTIRSKTGLVLGYKGLDETIRRAYVRCSPAPKRVTSDGLRIEANIPPRGDMTWE
                     ISIACEIKQRRSRVFPSYDRAYREADRSLQKAKTSDVHVHTSNEQFNHWLNRSLADL"
     gene            complement(839..2128)
                     /locus_tag="VN039_09435"
     CDS             complement(839..2128)
                     /locus_tag="VN039_09435"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV46224.1"
                     /translation="MQGRSGRLPYSAPVVENADNISVQDRPLLLTRDFGLVWSGQLIS
                     QIGDGVSKLALLWFVYAVTGSPLKTSVIGLLQTIPPIVLGPIIGVYVDRLPKKLLLIT
                     SDVLRAVLIGLIPCLIPVESFTVSVLYALVLLHAIATAVFGPALTAAIPAFVPKTQFT
                     AANALLQSTTSLGIIFGPALSGLGIAAYGSQEVLCLNAATYLISAAFLMLVRFAKPVA
                     SSPSPASTPTMLQDLIEGFRYGVVTQPRLLLLTGTAVLYTFGTSALTALFPVFARKML
                     GLGPVEVGYLWSALGVGLLLTSIGLLWVTDWMLKDRMRLIMGASVLSGAALCALTQTL
                     DPYLAGFFMGIVGCGMGAFTPIAWGIIQELAPSQMIGRVLGLYGTGAMTAAIGGISAF
                     GWITEQQGPETGLLGIALVLLITALAATRMSRASAGA"
     gene            complement(2128..>2440)
                     /locus_tag="VN039_09440"
     CDS             complement(2128..>2440)
                     /locus_tag="VN039_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWV46225.1"
                     /translation="ATEIAQAVPGVKTVVNKLAVEKDLAKTLLKKQDEILTTLIKERF
                     SKSATLKAANFEVKTEDGVVQLNGTVRFQVIALEAAEAARQVPGVRAVNTERIRLEGE
                     S"
BASE COUNT          561 a          698 c          687 g          494 t
ORIGIN      
        1 gaagatcggc gagcgaccgg ttcaaccagt gattgaattg ctcattcgaa gtgtggacgt
       61 ggacatcgga agttttggct ttctgtaacg agcgatccgc ctcacgatag gcacgatcgt
      121 aggagggaaa cacccgactc cgccgttgct tgatttcaca ggcgatcgag atctcccacg
      181 tcatgtcgcc gcgcggagga atattcgcct cgatcctcaa tccatcggat gtcacccgtt
      241 tcggcgccgg agagcaccgg acataagctc gacgaatggt ttcatcgagt cctttatacc
      301 caagcaccag tccggtcttc gatcgaatgg tgttgagccg ccatccgcga cgaggccgct
      361 gtcggcctct cgcttcgaag agatcggcga aatccgtatc cattgtcatc gtgagcaaga
      421 ggggaaatgt cctatccccg taattatgaa cttggatgcg ctcgtgacag gtcccttgcc
      481 agagaaaccg ggtacggcat agatggagcg tgcctcgcgg taccggccgt tgtccgtcca
      541 ttaggtccgg gttggtaaga tcaatccgaa cgagcgcatt gtcctctttc accatggcgc
      601 tgagcaacat cggacgctgt gccccgagcg aaagttcgaa tcgggatagg aaccgcgtcc
      661 cttcatgata aagcccctgc ggtccgggaa gtgcttggtg catatcaccg taccgatcga
      721 acactgcgaa ggtctcgccc tgcttcaaca cttccgtgcg gccgtcggcc aatgacgatg
      781 aagcgaggat gtaatattga tctttcactc tgaccacatt gttcatgacc tgtttccttt
      841 acgcgccggc acttgctcga ctcatccgtg tggcggccaa cgctgtgatc aacagcacaa
      901 gagcgatccc gagtaagcct gtttcagggc cctgttgctc cgtgatccat ccgaaggccg
      961 agattccccc aatggctgca gtcatggcac cggtcccata gagtcccaac acccgtccga
     1021 tcatctgact cggcgcaagc tcttggataa taccccatgc tatcggagta aaggcaccca
     1081 taccgcaacc gactattccc atgaagaaac cggcgaggta tgggtcgagc gtctgcgtca
     1141 acgcacagag ggcggcaccg ctcaacacac tggcgcccat aatcaggcgc atccgatctt
     1201 tgagcatcca gtccgtcacc cacaaaagcc cgattgacgt cagtagcaat ccgaccccga
     1261 gtgccgacca taaataaccg acctcgacgg gacccaatcc gagcatcttc cgtgcgaaca
     1321 ccgggaagag agcggttaat gcactagtgc cgaaggtata gaggactgcg gtaccggtca
     1381 agagtaacag tctcggctgg gtaacgacgc cataacgaaa tccttcgatc agatcctgca
     1441 gcatggtcgg ggtagatgca ggtgacgggg aacttgcgac aggtttggca aaacggacca
     1501 gcatcagaaa cgcagccgat atcaagtacg tcgccgcgtt gaggcataag acctcttgtg
     1561 acccgtacgc ggcaatgcct aagccgctca gcgccggacc aaaaataata ccaaggctgg
     1621 tcgtactttg aagaagcgcg ttggcggccg tgaactgcgt tttagggaca aacgccggga
     1681 tggcggcggt gagagccgga ccgaacaccg ccgtggcaat cgcgtgcagg aggaccaacg
     1741 cgtaaaggac agataccgta aaggattcca cgggaatcag gcacggaatc aacccaatca
     1801 atactgcacg tagcacatcg ctggtgatca gcaggagttt cttcgggagt cgatcgacat
     1861 acacaccgat gatcggaccc agaacgatcg gggggatcgt ctgtagcaga ccgatcacag
     1921 acgtcttgag cggggatccg gtgacggcat agacaaacca cagaagcgcc agcttggaga
     1981 caccgtcgcc tatctgagaa atgagttgcc cggaccagac gagtccgaaa tcacgggtca
     2041 ggagcagtgg gcgatcctgg actgagatgt tgtctgcgtt ctccaccacg ggggcagagt
     2101 aaggcaagcg gccggagcgg ccttgcatca gctctcgccc tccagccgta ttctctcggt
     2161 gttcacggca cggacgcccg gcacttgacg agcagcctcc gcagcttcga gggcgatgac
     2221 ctggaaacgc accgttccgt tgagctggac gacaccgtcc tcagtcttca cctcgaagtt
     2281 ggctgctttt aaggtcgcgc ttttggagaa acgttctttg atcaaggtgg tgagaatttc
     2341 atcttgtttc ttcaacagcg ttttcgcgag gtctttctcg actgcaagct tgttgaccac
     2401 ggtcttgacg cctgggacgg cttgcgcgat ttccgtcgcg
//
LOCUS       DATMTI010000594         1869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_192764, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000594 DATMTI010000000
VERSION     DATMTI010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192764"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="VN039_09445"
     CDS             complement(<1..1850)
                     /locus_tag="VN039_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV46226.1"
                     /translation="MISGDVPDSLKNKRIIAMDIGAMVAGAKFRGEFEDRLKAFLKEV
                     TDSQGQVVLFIDELHTIVGAGAAEGSVDASNMLKPMLARGELRTVGATTIDEYRKYIE
                     KDAALERRFQPVLVGEPSVEDTIAILRGLKERYEVHHGVRITDGALVAAAVLSQRYIT
                     DRFLPDKAIDLVDEAASRLKIELDSMPTEIDVIERQVMQHEMERQALKKEKDPASKER
                     LKKLEKELAELKEKSNALKAEWQKEKAVIDEQRKVKEEIDRFRTELERAQRRGELANA
                     SEIQYGRIPELEKKLSDLTAKSAKTQKRLLREEVTEEDIAEVVSHWTGIPVSRLQEGE
                     REKLVKLESHLHLRVVGQDEAIKAVSNAVRRARAGLQDPNRPLGSFIFLGPTGVGKTE
                     LARALAEFLFDDENAMIRIDMSEYMEKHTVARLIGAPPGYVGYEEGGQLSEAVRRKPY
                     SVVLFDEIEKAHPDVFNVLLQVLDDGRITDGQGRTVDFKNTVVIMTSNIGSQYITEEE
                     SPEARRRLVMDALRAHFRPEFLNRVDEIIIFDRLSEEELKKIVEIQLGRLSKRLEQQK
                     ITLKLSDAAKALLAREGYDPVYGARPLRRAIQRKILDPLSIDILEGKVREG"
BASE COUNT          382 a          563 c          460 g          464 t
ORIGIN      
        1 ccttcgcgca ctttcccttc gagaatgtcg atactgagcg gatcgagaat ttttctttgg
       61 atggcgcgac gcaagggtcg cgcgccgtaa acaggatcgt acccttcgcg cgccagcagt
      121 gctttcgccg catctgacag cttgagtgtg atcttttgtt gctcgagacg tttcgacaac
      181 cggccaagct gaatctcgac gatcttcttt aactcctctt cactgagccg gtcaaaaatg
      241 atgatctcgt cgacacgatt caaaaattca ggtcgaaaat gtgcgcgtaa cgcgtccatc
      301 accagtcgcc gacgtgcttc gggagactct tcctcggtga tgtattgcga accgatgttg
      361 gaagtcatga tgacgaccgt gttcttgaaa tcgaccgtcc tcccttgccc gtcggtgatc
      421 cggccgtcgt cgaggacctg cagcagcacg ttgaacacgt ccggatgcgc cttttcaatt
      481 tcgtcaaaga gcacgaccga atacggcttc cgacgaacgg cctcagaaag ttgaccgcct
      541 tcctcgtagc cgacgtagcc cggcggagcg ccgatcaacc gcgcgacagt gtgtttctcc
      601 atgtattcac tcatgtcgat acggatcatt gcgttctcgt cgtcgaataa aaattcggca
      661 agtgcgcggg ccaattcagt tttcccaacg cccgtgggac cgagaaagat gaatgaaccc
      721 agtggacgat ttggatcctg cagacctgcc cgagcgcgac gcacggcgtt ggacaccgcc
      781 ttgatcgcct catcctgacc aacgacacgc aggtgcaagt ggctctcaag ctttaccagt
      841 ttttcacgct cgccttcctg caaacgcgaa accggaatac ccgtccagtg cgacacaacc
      901 tcggcaatgt cttcctctgt gacttcttcc cggagcaaac gcttttgggt cttggccgat
      961 tttgcggtga gatcggacag tttcttttcc agttcgggaa tgcgcccgta ttgaatttcg
     1021 ctggcattag ccaattcccc gcgacgctgg gcacgttcca gctcggtccg aaaacgatcg
     1081 atctcttcct tcaccttgcg ctgctcatcg ataaccgctt tttccttctg ccattctgcc
     1141 ttgagtgcat tcgatttttc cttgagctca gccagttcct tctccagttt tttcaaacgc
     1201 tctttgctgg ccggatcttt ctctttcttg agcgcctgcc gttccatttc gtgctgcatg
     1261 acctggcgct cgatcacgtc gatttcagtc ggcatggaat ccagctcgat cttcaaacga
     1321 gatgccgctt cgtccaccaa atcgatcgcc ttatcaggca ggaaacggtc cgtgatgtaa
     1381 cgctgtgaca ggacagctgc tgcgaccaat gctccatcgg taatccgcac cccgtggtgg
     1441 acctcgtaac gctcctttaa tccgcgcaga atcgcgattg tgtcttcaac actgggctca
     1501 cccacaagaa ccggctgaaa acgccgttct aaggcggcat ctttctcgat gtatttgcga
     1561 tattcatcga tcgtggtcgc gccgacggtg cgtaattccc cacgcgccag cattggcttg
     1621 agcatattgg acgcatcgac cgaaccttcc gccgcgccgg ccccaacgat cgtgtgcaat
     1681 tcatcgataa aaagaacgac ctggccctga gagtcagtga cttcttttaa gaaggctttg
     1741 agccggtcct cgaattcccc gcggaacttg gcgccggcaa ccatggcgcc gatatccatc
     1801 gcgatgatgc gtttattttt caaagaatcg gggacatcgc cgctgatcac gcgacgcgcc
     1861 aaaccttcc
//
LOCUS       DATMTI010000595         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_658835, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000595 DATMTI010000000
VERSION     DATMTI010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658835"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2066
                     /locus_tag="VN039_09450"
     CDS             <1..>2066
                     /locus_tag="VN039_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWV46227.1"
                     /translation="ALYGFSGVAQLAEYLETLLEQATPIPTAEWNRAVDSMRNITQSL
                     RVIVRTIGQGGAEDAEAVERCWASIKQREGETSDATASASSSSTIDEDYLFPALDAEI
                     LSYFIPEAEEYLATIDELIRVLREKRDDADSIYRLFRAAHTLKGSAYTVGFHVIGDVA
                     RPMEDCMVAVHEHRLPLGNDLLDMLAKAAELIRLILRHKSENTQLLRHDIPLLLNRLI
                     QVAGGTAASPAATDQSYRTDIGGSDSVQPTPVVGIEVPAADLSDRYLVPDLDPEVLSY
                     FIPEAQEYLELLEANLLRLDKDPQNKELINPLFRSAHTLKGSAYTVGFQSIGDLVHHV
                     EDFMGSVRDGSLSVLPGHTDLMLRAIDVVRVLMRRDLDKVDDTRQRFESARADLRRLD
                     QGVISETTPAHQQDDTSGTTAAGPSGGEESHIQDDGAQNEKSREEREVIRVSYARLER
                     LMNLVGELVIGRGRLEQRLRVLEQLSQQVLVFRSRLVDSVQSFADKHTFSYQDTSSSS
                     TAPASQGLPAFGDFGSLELDKYDDFNILARRIGEVTADINESMSQLDGSIQRTHDEMS
                     QLQHLTLLMRDEIAHARMVPIGTPFTRFRRAVREIARASNKEASLITSGEQTEVDTGV
                     VERLVDPLVHLVRNAVYHGIEPAADRVAKGKPAIGTVYLHAVHRGNSVIIEVEDDGAG
                     LDMDKI"
BASE COUNT          498 a          551 c          579 g          438 t
ORIGIN      
        1 cggccctgta tggcttcagc ggagtcgctc agcttgccga atacttagag acactgcttg
       61 aacaagcgac gccgattcct acagctgaat ggaaccgagc tgtagactcg atgcggaaca
      121 ttacgcaaag ccttcgggta atcgttcgaa ccatcggaca aggcggcgct gaggatgcag
      181 aagccgtaga acgctgttgg gcgtcgataa aacaacgaga aggagaaacg agcgacgcaa
      241 cagcgtccgc gtcctcctcg tcgacgatcg atgaggacta cctgttcccg gcgctcgatg
      301 ccgaaatctt atcctacttc atcccggaag ctgaggaata cctcgccacc attgacgagt
      361 tgattcgtgt ccttagagaa aagcgggatg atgcagactc gatctaccgg ctctttcgcg
      421 cggcgcatac cctgaagggc tcagcgtaca ccgtaggctt ccacgtcatt ggagatgtcg
      481 ctcgtccgat ggaagattgc atggtcgcgg ttcatgaaca tcgccttcct ctcggcaacg
      541 accttcttga catgcttgcg aaggctgccg aattgatccg gctcattctt cgtcataaat
      601 cggagaatac gcaacttctc cggcacgaca taccactcct tctcaaccgt ctcatccaag
      661 tggctggagg tacagccgcc tcacctgcag cgactgatca atcctaccgc accgacatag
      721 gtggctcgga ttctgtccag cctacgcctg ttgttggcat agaagtgcct gcagcggact
      781 tatccgatag gtacttggtt ccggatttgg atccggaagt gctctcctac tttatccctg
      841 aggcacagga gtatctggaa ctcttggaag ccaatctcct gcgattggac aaggacccgc
      901 aaaacaagga actgatcaat ccgttgttca gaagcgcgca caccttgaaa ggctccgcct
      961 ataccgttgg gtttcagtcc atcggcgacc ttgtccatca cgtcgaagac ttcatgggct
     1021 cggtgcgaga cgggagtctc agcgtattac ccgggcatac ggatcttatg cttcgcgcga
     1081 tcgatgttgt acgagtactc atgcgaagag acctcgacaa ggtagacgac acaaggcaac
     1141 gattcgaatc ggcgcgagcc gacctgcgac gattggatca gggtgtcata agcgagacga
     1201 cgccggcaca tcaacaggat gacacctccg gtacaacggc cgcaggaccg tccggaggtg
     1261 aggagtctca tatacaggat gatggagctc agaatgagaa atcgcgagag gaacgcgagg
     1321 tcatccgtgt cagttatgca cggctcgagc gattgatgaa tctcgtagga gaactcgtca
     1381 ttggacgcgg aagattggaa caacgattgc gagtcctgga gcaactgtca cagcaagtgt
     1441 tggtattcag gagtcggttg gtggattccg ttcagtcctt tgccgacaag cacaccttca
     1501 gttatcagga cacatccagt agctcgaccg cgcctgcgag tcaaggactt cccgcattcg
     1561 gtgattttgg cagtctcgag ctggacaagt acgacgattt caatattttg gctcggcgca
     1621 tcggggaggt caccgccgat atcaatgagt cgatgtcgca gttggatggg tccatccaaa
     1681 gaacccacga cgagatgagc caactgcagc acttgacgct gttgatgcgg gatgaaatcg
     1741 cccatgcccg catggttccg atcggcacac cgttcacgag gtttcgtcga gcggtcagag
     1801 aaatcgctcg agcttcaaac aaggaagcgt ccttgatcac atcaggcgaa cagacagaag
     1861 ttgataccgg agtcgtggaa cgattggtgg atccattggt gcatttggtc aggaacgctg
     1921 tttatcacgg catcgagccc gcggccgatc gagtcgcgaa agggaaacca gccatcggaa
     1981 ccgtctacct ccacgcagtt caccggggga actcggtcat catcgaggtg gaagatgatg
     2041 gagccgggct ggatatggat aaaata
//
LOCUS       DATMTI010000596         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_531966, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000596 DATMTI010000000
VERSION     DATMTI010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531966"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..808
                     /locus_tag="VN039_09455"
     CDS             377..808
                     /locus_tag="VN039_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWV46228.1"
                     /translation="MLWSTIMKSYREELWFETKTRRAYINITPHVEAVVKTSGIREGL
                     VLVNAMHITASVYINDDESGLLQDYDDFLERLAPQKAVYRHHDTGEDNGDAHLKRQVM
                     GREVVVALTEGKLDFGPWERIFYGEFDGRRRKRVLVKIIGE"
     gene            941..1954
                     /locus_tag="VN039_09460"
     CDS             941..1954
                     /locus_tag="VN039_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46229.1"
                     /translation="MLGWSRPDPLTRDLIHLINARRHDHGDTDDAIDTLQAFLEQGRE
                     HDLLTVLAALDEEMAEWLFDLVAEASCTLLLDGPNDEKPTYATMAALELPLQANLTAP
                     LKLKIDPIATADLLQKYLQLPAGTVVRVESRLLTDATLEMLNLQLFHDHLMTVTDSRR
                     VQSVAARLLPPVENTAFLLFELIGAPDPGLPDAWEDRQRREILEGLVKQCPELACAPD
                     TIEAPVYAAYAMFDAQNAVRLHHLADETAAVWRELDPLVRSRTIVHLHTQCGNGVYMP
                     VWTDLFDPELPDEHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVET
                     PDN"
     gene            2093..2662
                     /locus_tag="VN039_09465"
     CDS             2093..2662
                     /locus_tag="VN039_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46230.1"
                     /translation="MNSISAAGLILIWIIIWANFPSKEELFSAQSASSIVEAQRVPEL
                     DLTIPGVVGRPAESQAGAADSSSAATFGGLSVPRDRRAREIAEVKCEAEVQQYCPDSL
                     TGEDRRRCVLQRLKRLDVPCQQIVRQRLVRWREAEGYKLACIEDVKRVCLTVQPGDGR
                     ILQCLQEHEQDLSEACYQSLPKGRLNLRN"
BASE COUNT          613 a          743 c          805 g          606 t
ORIGIN      
        1 ggcggcttca tgggcgaagg tgatcgaatc gtggttgtct gttccagctc ctcttccgtg
       61 aattccagat ccggttccgg ctgacgagca aaaagtagac tccaattcag aggactgccg
      121 acgaacgacg gacccccagc gatagccaac atcgagcacc tccctctagc ggatagcatc
      181 gaagaatctg aaaaggtact tatcaccaag gccaggggct gtcaattttg taggcgtttc
      241 cgacagggtt cattcgatac agatagggac gccacaggat tggccgatcc catcatacct
      301 cttcgtcggt gtcggaacat gcaagatgaa tggaatcgct cggccctctt gcatgtctgt
      361 cgagacggcg ggcacaatgc tgtggtcaac gatcatgaag tcctaccgcg aagaactctg
      421 gttcgagaca aagaccaggc gcgcctacat caatatcacg ccccacgtcg aggcagtggt
      481 aaaaacgagt gggatacggg aaggcctcgt cctggtgaac gcgatgcata ttacggccag
      541 cgtatacatc aatgacgatg agtccggcct gttgcaggac tacgacgatt tcttggaacg
      601 gctcgctcca cagaaggcgg tgtatcgtca tcacgacaca ggcgaagaca acggtgatgc
      661 tcatctgaag cgacaagtaa tggggcgaga agtcgtcgtc gcgctcacgg aaggaaaact
      721 cgacttcggt ccgtgggagc gaatttttta tggcgagttc gacggacgtc gtcgtaagcg
      781 ggttctcgtg aagattatcg gtgaataggg gcgtgcgtcg atcatgtgcg tggcgagtcg
      841 caagtaatcg tccggaggct tgcatgggta ggaaaagttt ggcaacatgc tttttgagtt
      901 gtcgagccat ggctgacggc tcgtacagta gggagcctcg atgttaggtt ggtctcgccc
      961 cgatccgctc acacgggatc tcattcatct catcaatgcc cgtcgccacg atcatggcga
     1021 cacagatgat gcgatcgata ctttgcaggc gtttcttgag caaggacgtg agcacgattt
     1081 gctgaccgta cttgccgcgc tcgatgaaga aatggccgaa tggctgttcg atctagtcgc
     1141 cgaggcaagt tgcacgttgc tgttggacgg cccgaatgac gaaaagccga cgtatgcgac
     1201 gatggcggct ctggaactcc ccctgcaggc caacttaact gccccgctca agctgaagat
     1261 cgatccgatc gccacggccg atctcttgca gaagtatctc cagctccccg ccggaacggt
     1321 tgttcgcgtc gagtcgcgcc tcttgaccga tgccaccttg gagatgttga atcttcaatt
     1381 gtttcatgac cacctgatga cggtcacaga ttccaggcgt gtccaatccg tggcggcgcg
     1441 gttgcttcct cccgttgaaa atacggcatt ccttctgttt gagctgatcg gcgctccaga
     1501 tccaggtttg cccgatgcgt gggaggatcg acagcgccgt gagattctcg aagggctggt
     1561 gaagcaatgc ccggagctgg catgtgcgcc tgacacaatt gaggctccgg tgtacgccgc
     1621 ctatgccatg ttcgacgcgc agaacgcagt acgcctccac cacctcgccg atgagaccgc
     1681 tgctgtatgg agagagttgg atcccctcgt gagatcccgc accattgtgc acctccatac
     1741 acaatgtgga aatggggtct acatgcctgt gtggaccgac ctattcgatc cggagctgcc
     1801 ggacgaacac ggccctgttt ggacttcccc cgatgtctgg gtcttcaccg ccgatttgcc
     1861 gattgaatgg ccgggtgaga tgttgaccaa tcggttattg gcggaaggct gtaccaggtt
     1921 cgaaaatcac atcgtcgaaa ccccggacaa ctagtttctc tccattccag tctgttcggt
     1981 cgacagcacg gtcttgcaca aaactcctgt ttcgatgatt ttcccgcggc tttattttat
     2041 cctcaccgtg atgaagactc ccaacaccgg gaaacgatct aagaaagcga tcatgaactc
     2101 catctcagcg gcggggttga tcttaatctg gatcatcatc tgggcgaatt tcccgtcgaa
     2161 ggaagaactc ttctcagcgc aatcggcttc gtcgatcgtt gaagcgcaac gcgtgcctga
     2221 gctcgacctg acgatcccgg gcgtggtcgg tcggccggct gaaagccagg cgggagcggc
     2281 agactcgtca tcagcggcca cttttggggg gttgtctgtc ccgcgcgaca gacgtgccag
     2341 ggaaatcgcg gaagtgaagt gcgaggccga agtccagcag tactgccccg attccctgac
     2401 aggcgaagac aggcgacgtt gcgtgctgca gcgtctcaag cgattggatg tgccgtgtca
     2461 acagatcgtc cggcaacggc tggtacgctg gagggaagcg gaaggatata agcttgcctg
     2521 cattgaagat gtgaagcggg tctgtctgac ggtgcagccg ggtgatggcc gcatcctgca
     2581 atgtttgcag gaacatgaac aggatctctc agaagcttgc taccaaagct tgccaaaagg
     2641 gcgactcaat ctccgaaact gaatcgtaca tcaggtacgg caagccgcac agctttgccg
     2701 gtgtcgcttt cgactgccga cgtggtgcga ttcatagaca gcgatcggaa agattggctg
     2761 gaaacct
//
LOCUS       DATMTI010000597         5789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_786522, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000597 DATMTI010000000
VERSION     DATMTI010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786522"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /gene="gcvT"
                     /locus_tag="VN039_09470"
     CDS             complement(<1..959)
                     /gene="gcvT"
                     /locus_tag="VN039_09470"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWV46231.1"
                     /translation="MQHTPLIAQHRAAGAKLVDFAGWEMPIHYTGVVDEYHTVRSKAG
                     LFDVSHMGRIVVTGSGAESFLQRMTTNDIAALRPMQAQYSMVCNEQGGIKDDIFVYRL
                     ANGGQFLLCVNASNRDKIVSWLTEHSRGVSGCQISDRSAEIAQIALQGSAARAIVAVL
                     GLSMLEQLKLRESTTVRVADVECLAARTGYTGEFGYEFYVHGQADAVWNKLLDTGKSF
                     GLKPAGLGARDLLRLEMGYLLYGNDIDERTTPIEAGAEWAVRFEKGEFVGKTALLSQK
                     QAGLSRRFVGVELLEKGVPRHGFKILSSSSPQSPIGEVTSGNLS"
     gene            complement(967..1410)
                     /locus_tag="VN039_09475"
     CDS             complement(967..1410)
                     /locus_tag="VN039_09475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWV46232.1"
                     /translation="MKPLRRAYVCLIVVITFLLGWGNAMAGNEKETSMQEVTTSSGLK
                     YVDQVIGVGDAAVVGKTASVHYTGWLENGKKFDSSVDRGQPFSFPLGAGRVIKGWDEG
                     VQGMKVGGKRKLTIPSDLGYGSRGAGGVIPPNATLIFDVELLGVR"
     gene            complement(1458..2645)
                     /locus_tag="VN039_09480"
     CDS             complement(1458..2645)
                     /locus_tag="VN039_09480"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HWV46233.1"
                     /translation="MQFAVAISKTAETESAAWAAAEDIREQLNAAPLDLACVFFSVHH
                     AARADLLAHVLTEVLRPRLLIGCSGEGVIAGAEELETAPAITVWAAVLPNVNLHSLRS
                     SFSPTHDQFRLSGWPEPGGVEEASFLLLADPFTVPVQDILGILEERYPGTRAIGGLAG
                     GGQEAGMNRLLFNDQVLDSGLVGVRLSGAVDIRPIISQGCRLIGDRFVVTKAERNLIH
                     ELGGAPALERLQAVFESLAEEDRRRAHRALHLGIVIDEHRNRFERGDFLIRNLLGADR
                     TTGAVAIGDVVQVGQTVQFHLRDAESATEDLHSLLATDRADHRHPPLGALMFSCCGRG
                     RGLFGRPNHDVAATMERLGPIPIAGFFAQGEIGPIGNRNFLHGYTASLAIFAERDSQP
                     NSG"
     gene            complement(2723..2950)
                     /locus_tag="VN039_09485"
     CDS             complement(2723..2950)
                     /locus_tag="VN039_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46234.1"
                     /translation="MTQYQIGGGLQLLTAVQKTEAFGEFLKTRMIHALETEDPTELHY
                     LLAQVDDYHSYLWRYYKKLAQTRAQRMDPGV"
     gene            complement(3054..5666)
                     /locus_tag="VN039_09490"
     CDS             complement(3054..5666)
                     /locus_tag="VN039_09490"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HWV46235.1"
                     /translation="MKLSFHGAARSVTGSRHMLEVPGFRLLFDCGMFQGRREEAVRRN
                     RDLGFESKSIGAVLLSHAHVDHSGALPVLPQQGFSGKVYLTRASADLTSIMLEDSARV
                     QENDCRYVNKQERRRGKICIRPLYDGDDVRKIVRRFEGERYGAQLKIAPRITASFHDA
                     GHILGSAAVRVKYTARGITTTVLFSGDLGRSRMPILRDPEPPPPCDVLILESTYGDRL
                     HEQVGEEMKKKAQDLLDHARTHKSKIIVPAFAVGRTQELVMRIKELVGEGRVDPVPIY
                     IDSPLADKATEVFKRHPECYDDETMKTFSSDGDVFASRYIHFVSSSEGSKRLNAMRGP
                     CVIISSSGMCEGGRVIHHLKHARFKMRRTSSSLSAFKRNIRSAANSSKGGTWCRFSAC
                     RRQDGPRSSSSMVCRLMPIGTISWRMCEPFTQGPAPSSSYTAKRNRLFPWVRRSKRNI
                     RPPMCGSLIRAVRMISRSRHFIGAGGRQPARLLVLSCAAALLAGCLSTAAAEPKQERQ
                     RLRDLGIVVGQYPPGPLNAITDITGVKVGHTTLISGEGILKPGQGPIRTGVTVVIPRD
                     DVWHKKVPAGSFVLNGTGEMTGLAWVAESGFLEYPIALTNTLNVPRVANGVMSWMVKR
                     YPAIGIEDDTLTPVVAECDDGRLNDIQGRHVSEQHVMTALDGATGGPVAEGTVGAGTG
                     MVSYGFKGGIGTSSRKLPEREGSYMVGVLVNANHGRRPELLVSGVPVGKLYEPPAQMS
                     EALAPGQSEGSIIIVIATDAPLDSRQLIRLAKRAALGLARTGSTARHGSGDFILAFST
                     ANVIPHYPKDPTYSLTHLADTHLNPLFAATVEATEEAILNALTMATTVTGRDHRRIEA
                     IDLDRLKTVMSGSR"
BASE COUNT         1213 a         1727 c         1616 g         1233 t
ORIGIN      
        1 gaaagattgc cgctggtgac ttctccgatg ggagactgtg gtgaggatga agagagaatc
       61 ttgaagccgt ggcggggtac gcctttctcc agcaactcga caccgacgaa tcggcgggac
      121 aggcccgctt gtttttgact caacagggca gtctttccga caaactcccc cttctcgaaa
      181 cgcacggccc attccgctcc ggcctcgatc ggggtcgttc gttcatcgat atcgttgcca
      241 tagagcagat aacccatttc gagccgcaag agatcccgtg cgccgagacc tgccggtttc
      301 aaaccgaacg acttgcctgt gtccaacagc ttattccaga ctgcgtcggc ttgtccgtgc
      361 acataaaact catagccgaa ttctccggta tagcccgttc gagccgccag gcactcgaca
      421 tccgcgactc gcaccgtcgt tgattctctg agcttgagtt gttccagcat tgacaatccg
      481 agcaccgcga caatcgctcg cgcagccgaa ccttgcaagg cgatctgcgc gatctctgct
      541 gaacggtcgg agatctgaca acctgaaact ccccgactgt gctcggtaag ccatgatacg
      601 atcttgtctc gattggatgc gttgacgcac agaagaaatt gtccgccatt cgccaaccga
      661 taaacgaaga tatcgtcctt gataccgccc tgctcgttac agaccatcga gtactgcgcc
      721 tgcatgggcc gcagcgcagc gatatcgttc gtcgtcatgc gctgaaggaa agattccgca
      781 cctgatccgg tcacgacaat cctccccata tggctgacat cgaacagacc ggccttgctt
      841 cgaacggtgt gatactcatc cacaacgccg gtgtagtgta tgggcatttc ccaccctgca
      901 aagtcgacca gtttcgcccc ggcggcgcga tgttgtgcga tgagaggggt gtgctgcacc
      961 ggacggttac cgcacaccga gcaattcgac gtcgaaaatc agcgtggcgt tcggtgggat
     1021 cactccgccg gcaccacgcg atccgtaccc caagtcggat gggatggtga gtttgcgctt
     1081 gccccccacc ttcatcccct gcaccccttc gtcccagccc ttaatgaccc gcccggcacc
     1141 gaggggaaac gagaatggtt gcccacgatc gacggaacta tcgaactttt ttccgttctc
     1201 cagccaaccc gtatagtgta cgctcgccgt ctttccgacg accgccgcat ctccgacgcc
     1261 gatcacctga tcgacgtatt tgagcccaga cgatgtggtg acttcctgca tgcttgtctc
     1321 cttctcattc ccggccatgg catttcccca tcctagtaaa aacgtgataa ccacgatcag
     1381 acagacatag gcacgacgca gaggtttcat aaactaccct ccttcgctca ctgtatggca
     1441 tgagagagac tactcggtca accggagttt ggttgggaat ctctctcggc aaagatcgcc
     1501 aggctagccg tgtatccgtg caagaagtta cggtttccga tgggccctat ttcgccttgc
     1561 gcaaagaacc ctgcgatggg aatcggtccc aggcgttcca tagtcgcagc cacgtcatgg
     1621 ttcggtcgtc caaaaagccc tcgaccacgg ccgcaacagc tgaacatgag tgcgccgagc
     1681 ggaggatgcc ggtgatcggc tcgatcggtc gccaacagag agtgcaaatc ttccgtggct
     1741 gattccgcgt cgcgcagatg gaactgcacc gtctggccta cttgcaccac atcgccgatc
     1801 gcaaccgcgc ccgtcgtacg gtcggctccg agcagattcc gaatgagaaa atctcctcgc
     1861 tcgaaccgat tccgatgttc gtcgatcacg ataccgagat ggagcgcccg atgggcgcgg
     1921 cgtcggtctt cctccgccaa cgattcaaag acggcctgta accgctccaa agccggcgcg
     1981 cccccgagtt cgtgaatcag attgcgttcc gctttcgtca cgacaaaccg gtcgccgatc
     2041 agacgacacc cctgtgagat gatcggtcga atatccacgg ctcccgatag acggacgcct
     2101 actaacccgc tatcgaggac ttgatcgttg aagagcaacc gattcatgcc ggcctcttgg
     2161 ccacctccgg ccaatccgcc gattgccctc gttccaggat agcgctcttc caaaatccca
     2221 agaatatctt gaactgggac ggtgaatgga tctgcgagca ggaggaacga cgcctcttca
     2281 actccccccg gctcaggcca tcccgagaga cgaaattgat cgtgcgtcgg ggaaaacgac
     2341 gatcggagtg agtgcagatt gacgttggga agtacggcgg cccagacggt gatcgccgga
     2401 gccgtttcca attcctctgc tccggcaatc acgccctctc cgctgcagcc gatcagtagc
     2461 ctcggacgaa gaacctctgt taacacatga gccaagaggt cggccctggc tgcatgatga
     2521 acggaaaaga aaacacaagc gaggtcgagt ggggcggcat tcagctgctc ccgaatgtcc
     2581 tcggccgcag cccaagccgc ggattccgtc tctgctgttt tcgagatggc cactgcaaat
     2641 tgcaaggtcg cgcctcgcga tgttgaaagg caggtgagcc cggcacacgt gcgggtcgcg
     2701 ggagttcgga gcgcgatggg agtcagactc ctggatccat ccgctgagct cgggtctgcg
     2761 cgagtttctt ataatatcgc cacaggtaag aatggtagtc gtccacctgc gcaagaaggt
     2821 agtgcagttc cgtcgggtcc tcggtttcca gggcatgaat cattctcgtt ttcaggaact
     2881 cgccgaaggc ctcggtcttc tgcaccgccg tcagcagttg caacccgccc ccgatctggt
     2941 attgggtcat accatccctc ctggttaacg ttcaaaagga taacgagaga atcgccacat
     3001 ttgcaagctt ggctcaattt acaagagggt ctcagaaatt ctgttgatcc gtttcatcgt
     3061 gaaccggaca tgactgtttt gaggcggtcg aggtcaatgg cttcaatgcg gcgatggtcg
     3121 cgaccagtaa ccgtagtggc catggtgagt gcattgagaa tagcctcttc tgtggcctcg
     3181 accgtcgctg caaagagcgg attcagatgg gtgtcggcga gatgtgtcaa gctgtaggtt
     3241 gggtctttgg gatagtgagg aatcacgttt gctgtcgaga aagccagaat gaagtcgccg
     3301 ctcccgtgcc gtgcggtgga gccggtgcga gccaagccga gcgcggcgcg tttggccagc
     3361 ctgatgagct gccgactatc caagggcgca tcggtggcaa tgacgatgat gatcgagcct
     3421 tcactctgcc ccggtgcgag ggcttcagac atctgagcgg gcggctcata gagcttccct
     3481 accggcacgc cactgacgag cagttccggt cttcgtccgt gattcgcgtt gaccagtacg
     3541 ccgaccatat agcttccttc tctctcgggc agctttctcg atgaagtgcc gattccaccc
     3601 ttgaagccgt aggagaccat tcctgttccg gcgccgaccg tgccttccgc caccggcccg
     3661 ccggtcgcgc cgtcgagcgc cgtcatgaca tgctgctcgg acacgtggcg cccctggata
     3721 tcattcaacc ttccatcgtc acattcagcg acaaccggtg tgagcgtatc gtcctcgatc
     3781 ccgattgccg gatatcgctt gaccatccaa ctcatgacgc cgttcgcgac gcgcggaacg
     3841 ttgagcgtat tcgtgagggc gataggatat tcaagaaatc cggactcggc cacccaggcg
     3901 aggccggtca tttcgccggt accattaagg acaaacgagc ctgccggaac cttcttgtgc
     3961 cagacatcgt cgcgcggaat gacgaccgtc acgccggtgc gtatagggcc ttgccccggc
     4021 tttaaaatcc cctcgccgga gatcagtgtg gtatggccga cttttacgcc tgtaatgtcg
     4081 gtaatcgcgt tgagggggcc aggcggatac tgtccgacga caatccccag gtcgcgtaga
     4141 cgttggcgtt cttgtttcgg ttctgctgca gcagtggaga gacaaccggc aagcagcgca
     4201 gccgcgcagg acagcaccag cagcctagct ggttgtcggc cacccgctcc gatgaaatgc
     4261 cgagacctag atatcatgcg tactgccctg atgagggatc cgcacatcgg tggtcggatg
     4321 ttccgtttgg atcgccgtac ccaaggaaag agcctgtttc tcttcgccgt gtacgatgaa
     4381 gatggtgctg ggccttgggt gaatggctcg cacatacgcc aggagatcgt tccgatcggc
     4441 atgagccgac agaccattga acttgacgat ctgggcccgt cttggcgtcg gcacgccgaa
     4501 aatcggcacc acgtcccacc cttcgacgag tttgcggccg agcgtatgtt ccgcttgaaa
     4561 gccgacaaag acgatgatgt tcgcctcatc ttgaatcgcg catgtttgag atgatgaatg
     4621 acacgaccgc cctcgcacat gcccgatgag gagatgatga cacaggggcc gcgcatggcg
     4681 ttaagccgct tgctaccctc ggaagaggac acaaaatgga tgtagcggga ggcgaagaca
     4741 tcgccatcgg atgagaaagt cttcatggtt tcatcgtcat agcattcagg gtgtcgtttg
     4801 aatacctccg tggccttatc ggccagcggt gaatcgatgt agatcggaac cgggtccacg
     4861 cggccttcac cgaccagttc cttgatccgc atgaccaatt cctgtgtccg ccccaccgca
     4921 aaggcaggca cgatgatctt gctcttgtgc gtccgcgcat gatcgaggag atcctgtgct
     4981 ttcttcttca tttcttcacc gacctgttca tgcaatcggt cgccatacgt cgattctaga
     5041 ataagaacgt cgcagggtgg cggcggttca ggatcgcgca agatcggcat acgggatcgc
     5101 cccagatctc cgctgaacaa gaccgtggtg gtaatgccgc gcgccgtata tttcactcgg
     5161 acagcagccg atcccaagat atggccggcg tcgtgaaacg atgcggtgat ccgaggcgcg
     5221 atcttaagct gagcgccata tcgttctccc tcaaacctgc ggacgatctt ccgcacgtcg
     5281 tccccatcgt acaacgggcg aatgcagatc ttcccgcgtc tgcgctcctg tttgttgaca
     5341 taccgacagt cgttttcttg cacacgagct gaatcttcga gcatgatgct cgtgagatcg
     5401 gccgatgctc tggtgaggta gacttttccg gaaaacccct gctgcggcaa aaccgggagg
     5461 gcgcccgaat ggtcgacatg ggcgtgcgag agcaagacgg cgccaatgga tttcgactca
     5521 aaccccaagt cgcggtttcg ccggaccgct tcttctcggc gcccctgaaa cattccacaa
     5581 tcgaacagca gccggaagcc tggtacttcc agcatgtgcc gactgcctgt gaccgaacga
     5641 gcggcaccat ggaatgagag tttcatgcgg tgggtactcc attgtggcgt gcgatcaggt
     5701 cccaagcctc ctgagccgtc tcggcgtagt gaaacaagtg aagatctgtc tgcatgatca
     5761 agccgtactc aacgagcagt tgccagttg
//
LOCUS       DATMTI010000598         1897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_235827, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000598 DATMTI010000000
VERSION     DATMTI010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235827"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..683
                     /locus_tag="VN039_09495"
     CDS             120..683
                     /locus_tag="VN039_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWV46236.1"
                     /translation="MLKILFSLLGLVGLLVLGGGIWLGYGLYASGFSARVEPHPLEVT
                     VVRQFRRFAIPKTVRNMPNPVPMSPAALIEGLDHFADHCAGCHANDGSGETQIGKDVY
                     PRVPDLRRQEIQSMSDGELFFIIRNGIRFTAMPAWAEGDMDKDRGSWKLVHFIRHLPH
                     LTHEELERMQSLNPKAPHHDEGAGHHH"
     gene            750..1037
                     /locus_tag="VN039_09500"
     CDS             750..1037
                     /locus_tag="VN039_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46237.1"
                     /translation="MLPTIVMAAGTDVHVLGIVAAIDEKHIEVKTAKGPTVSVLLNKQ
                     VKFKNKSNSKSNDPPVEGDRVIIEATKDDKTKKMSATVVHYSPVKTASPPQ"
     gene            1041..1499
                     /locus_tag="VN039_09505"
     CDS             1041..1499
                     /locus_tag="VN039_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWV46238.1"
                     /translation="MPTIRFERAVHESPFHGGVRHALMRILITGIAVFLAIAIIPGLE
                     ADSLTAGIAAVLVLTFLNVILRPILFLLTLPLIVFTLGLFLVVLNALLLEFTAFLVKG
                     FIVSGFWPAVGGALIISLVTTILNSWTVDRRPLPDFQDEPRRPPKIINPD"
     gene            1636..>1897
                     /locus_tag="VN039_09510"
     CDS             1636..>1897
                     /locus_tag="VN039_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46239.1"
                     /translation="MVHRFRHILFVAVLVGLFVQIAWSDERPAPKSLWQTVPAPPTGQ
                     PSIPSKPWVLREREIVIDLPLLHVLKDAGARPLPRITVELFDK"
BASE COUNT          417 a          537 c          502 g          441 t
ORIGIN      
        1 acctgcctgt cttgatcatc ttgatcgttc ggctcgctcg ggccaataaa tccccggctc
       61 gatgagtcgg tggtattcat cttgcggaat ttgtatagtg ataaagagaa tctgctccta
      121 tgctcaagat cctcttctca ctccttggtt tggttggtct cttggtgctc ggcggaggaa
      181 tctggctggg atatgggctg tatgcaagcg gcttcagcgc cagggttgag ccgcatccgc
      241 ttgaagtaac cgtggtacga caatttcgtc gttttgccat tccaaagacg gtccgcaaca
      301 tgcccaatcc tgttcccatg agtccagccg cgttgatcga agggcttgac cattttgccg
      361 accattgcgc cggttgccat gccaatgacg gcagcggtga gacacagatc ggtaaagacg
      421 tgtatccaag agtaccggac ctgcgccgcc aagagattca atcgatgtcc gacggtgaac
      481 tgttctttat cattcgcaat ggcatccgct tcaccgccat gccggcctgg gcggaaggcg
      541 acatggataa ggatagagga agctggaagt tggtgcattt catacggcat ttgcctcact
      601 tgacccacga ggaactggag cgcatgcagt cgctgaaccc caaggcgcct caccatgacg
      661 aaggagcagg acaccatcac tagacgtctc ttatgttgaa gaactatgat gaccggataa
      721 gatactgcct gatcttcctg gcagcctgta tgttgccgac tatcgtcatg gctgccggca
      781 cggatgttca tgtgttgggc atcgtggcgg ccatcgacga gaaacacatt gaggtcaaga
      841 cagccaaggg gccgaccgtg tcagtcctgc tcaacaaaca ggtgaagttc aagaacaaaa
      901 gcaactcgaa gtcgaacgat ccaccggtgg agggcgaccg tgtcatcatt gaggcgacga
      961 aagacgacaa gaccaagaag atgtccgcaa cagtggttca ttattcaccc gtaaagactg
     1021 cgtcaccacc tcagtaaccg atgccgacga ttcgctttga aagggccgtg catgagtcac
     1081 cgttccacgg aggggtgcgc catgccctca tgcgcatcct catcactgga attgcggtat
     1141 tcctggcaat cgcgattatc cccggcctgg aggccgacag cctgacggcc ggcattgccg
     1201 cggtcctcgt cctcaccttc ttgaacgtca tccttcggcc tattctcttc ctgctcacct
     1261 taccgctgat cgtatttacg cttggactct tcctcgtagt tctgaatgcg ctgctgttgg
     1321 aattcacggc gttcctggtg aaagggttca ttgtctcagg attctggccc gccgtcggtg
     1381 gcgcgctcat catcagcctg gttacgacca tcctcaatag ctggaccgtc gaccgccgcc
     1441 ccctcccaga ttttcaggac gaaccgcgcc gtccgcccaa aatcatcaat ccggattgaa
     1501 gcattcgaca attcacgagg aattcgcgat tgacagaacg agcttctctt tcagttacgc
     1561 caatggaaag atctccccag ttatgctcaa attatcagga gcatcatagg gggctgggaa
     1621 gcaggggacg attccatggt tcaccgattc aggcatattc tcttcgttgc ggtgctggtc
     1681 ggtctctttg ttcagatcgc atggtcggat gaacgcccgg caccaaagag tctatggcag
     1741 accgttccgg ctcctcccac cggtcaacca tcgataccga gcaagccgtg ggttctccgc
     1801 gagcgagaga tcgtaatcga tctcccgctc ctgcacgtgc tgaaggacgc aggagccaga
     1861 ccccttccca ggattaccgt agagcttttc gataaac
//
LOCUS       DATMTI010000599         8176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_106706, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000599 DATMTI010000000
VERSION     DATMTI010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106706"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VN039_09515"
     CDS             <1..786
                     /locus_tag="VN039_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWV46240.1"
                     /translation="DVLRALLPLVQHKPDRDFLTKAQEKMHRWNQLMEERGTRTDMPM
                     KPQVIAHQLNDVLADDAIICCDTGTVTTWAARHIKIKGTMEFSASGTLATMACGLPYA
                     VGAAVAHPGRQVVCFAGDGGFTMLMGELATIVKYQLPVKIIVVKNNVLGMIKWEQLAM
                     EGNPQYGVQLQPIDFAAFARACGATGYTVDDPQAVPGVLREAFRHPGPAVIEAVVDPN
                     EPPLPGKATVDQALTFAKALARGTKDRWGIIKTVIEDKVREVV"
     gene            789..1931
                     /locus_tag="VN039_09520"
     CDS             789..1931
                     /locus_tag="VN039_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWV46241.1"
                     /translation="MSSSKVAQQVDRRAAGAQRVEVPVDEITVSAYHVPTDFPESDGT
                     LAWNKTTLVLVEAKAADRHGVGYTYADTGTARLIKDTLAEVVKGRDTMDVPACWAAML
                     HEIRNLGRPGIVSTAVSAVDAALWDLKARLLNLPLVRLLGAAHAGAPVYGSGGFTSYS
                     IDQLQEQLGGWVAEGIPRVKLKVGTEPWKDVNRVKAAREAIGPDVQLFVDANGAYSRK
                     EALAFAGAFSSLNVSWFEEPVSSDDLEGLRLIRDRAPAGMDITAGEYGYDLFYFRRML
                     EAGAVDVLQADASRCGITGFLKAAALCEARCLPISGHCAPALHLHAACAATPLRHLEY
                     FHDHVRIEQMLFDGAVKPEGGVLYPDLSRPGMGLEFKRADAQAYAV"
     gene            1950..5072
                     /locus_tag="VN039_09525"
     CDS             1950..5072
                     /locus_tag="VN039_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HWV46242.1"
                     /translation="MLGQELVQELVKDIGRPPRRTTNTTAQVDAQALAAELRNIINGE
                     VRFDDGSRALYAAGGANYRQVPIGVVIPKSVEDIIGTVAACRRYGAPVLSRGGGTSLA
                     GQICNVAVVMDMSKYFNRILELDPHKRRARVEPGLVLDVLRNAAEQYHLTFAPDPSTH
                     NHCTLGGMIGNNSCGVHALMGGKTEENTEELEILTYDGLRMRVGKTSDDELERIIGEA
                     GRRGEIYQKLKTLRDKYADLIRERYPKIPRRVSGYNLQQLLPEHGFDVAKALVGTEGT
                     CVTVLEATVRLVYSPPKRITVLLGYPDVYQAGDHVPEILEYRPIGLEGMDQVLVENIK
                     LKGTHPNDIKILPEGQGWLLVEFGGETTEEADEQANRMISALKRHPNAPSIKLYDDPE
                     HEKIVWETRKSGLGATARVPGQPDTWEGWEDSAVPPEKLGQYLRDLRALFEKYRYGCA
                     LYGHFGQGCVHTRIDFDLKTKDGIKKFRSFIGEAADLVVSHGGSLSGEHGDGQSRAEF
                     LPKMFGDELVEAFREFKTIWDPQGKMNPHKIVDPYRVDENLRLGADYNPLPTKTHFSY
                     VEDEGSFARATLRCVGVGECRRGQQGTMCPSYRVTREEMHSTRGRARLLWEMLEGNPL
                     KDGWREEAVHDALHLCLACKGCKHDCPVNVDMATYKAEFLSHYYAGRLRPMHAYSMGL
                     IYWWSRLASLMPGIANFFTQTAGISDAVKTMGGISVHRQMPPFASQTFTEWFRRRTPH
                     NVGRPKVILWPDTFNNFFFPETAVAAVEVLEAAGFHVTIPNRSLCCGRPLYDFGMLDL
                     AKSMWRQILETLHEEIEAGTPIIGLEPSCVAAFRDELVGLCPQSEDAKRLKAQTMTLA
                     EFLESKAPHIELPQLPRKAIVHGHCHQKAIMGMSAEQKVLKRMGLDFQILDSGCCGMA
                     GSFGFKDGDHYDVSIKVGELVLLPEVRKAPKETLILADGFSCREQILQTTDRRGLHLA
                     QVLQMALRDGQRVGPGVPGWEYPERRYVNPPALHAPFTRKDAAVLFGAGALLLGGAAA
                     WLLSRRRHFR"
     gene            complement(5242..5688)
                     /locus_tag="VN039_09530"
     CDS             complement(5242..5688)
                     /locus_tag="VN039_09530"
                     /inference="COORDINATES: protein motif:HMM:NF016798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HWV46243.1"
                     /translation="MSNPVPPPQSGFLAKTLGTMMSDPPWAAKSFLAAGATTVAGLGA
                     WLSNMMSPALASAGGSFLGGFLLGWAFRKTLKLALLVTISLLALIAILKTTGWIHLDW
                     TLIQADVNRTLDWARGKAQGFKEVLTGYLPAAGAGGAGVFFGLRKK"
     gene            complement(5685..6545)
                     /locus_tag="VN039_09535"
     CDS             complement(5685..6545)
                     /locus_tag="VN039_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWV46244.1"
                     /translation="MRIDDQRESDNIEDRRGMGPARLGKGGLGIGTIVLALAVSYFTG
                     VNPLTVLNLLTGVQSMTEVSAPSEPGPVGVPQDRLGKFASVVLADTETTWRSLLGPRY
                     KDPRLVLFSGAVQSACGTTSSAVGPFYCPNDHRVYLDLAFFDEMAHRLGAAGDFAQAY
                     VIAHEIGHHVQNLLGVAEKVHRLQRQVSEVEGNALSVRMELQADCYAGVWGHHADRER
                     NLIEPGDFEEGLRAAAAIGDDRLQKMSRGHVQPESWTHGSSEQRMTWLKRGLESGDPS
                     VCNTFETNRL"
     gene            complement(6571..7227)
                     /locus_tag="VN039_09540"
     CDS             complement(6571..7227)
                     /locus_tag="VN039_09540"
                     /inference="COORDINATES: protein motif:HMM:NF022620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HWV46245.1"
                     /translation="MLRVILAVILLFMSFGLSSCDKAYMATMEKMGYAKRDILSSRVK
                     SARDAQEDAKKEIQSTLEQFGKVVSYEGGDLEDTYKKLSGELESSEDSAKSVRKRISD
                     VESVADALFSEWEEELGQYSSADLRRKSQAKLTQTKGRYREMLGAMKRAEQRIEPVLK
                     PLRDQVLYLKHNLNARALAAIKGELVKVDAQVDQLVKDMNRSIAEADKFIQSMEKESD
                     "
     gene            7389..7949
                     /locus_tag="VN039_09545"
     CDS             7389..7949
                     /locus_tag="VN039_09545"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWV46246.1"
                     /translation="MNMRLTSFNVWWWAVLVLATACSSQRVIPEELEPLVDRTVPFPE
                     VVAAPDSYQGKIVVFGGEVLKAKRLKDGTQIELLQLPLDKDERPILDRQQSQGRFLAV
                     HQEFLDPATIAEGTKMTIVGELSKAKVEHLDDVEYRYPVLIVKYLHTWPAKSYGSAYP
                     HPRFSIGIGGGTGGRFGGGGGFGIGF"
BASE COUNT         1703 a         2286 c         2510 g         1677 t
ORIGIN      
        1 gatgtgctcc gagcgctgct gccattagtc caacacaaac ccgatcgcga ctttttaacc
       61 aaggcacaag agaagatgca ccgttggaat caattgatgg aggaacgggg cacgcggacg
      121 gacatgccga tgaagcctca agtgatcgcc caccagctga acgatgttct ggcggacgat
      181 gccattattt gttgcgacac ggggaccgtg actacgtggg ccgcccgtca cattaagatc
      241 aagggcacga tggaattttc cgcttccggc actttggcca cgatggcctg tgggcttccc
      301 tatgccgtcg gcgcggccgt tgctcatccg ggccgtcagg tcgtgtgctt tgccggtgac
      361 ggcggcttta ccatgctcat gggggaattg gcgaccatcg tgaaatatca attgccggtg
      421 aaaattatcg tcgtcaaaaa caacgtgttg ggaatgatca agtgggagca gctcgcgatg
      481 gagggcaatc ctcaatacgg cgtgcaactc caaccgatcg acttcgccgc tttcgcccgc
      541 gcttgtggcg cgaccgggta taccgtcgat gatcctcaag ccgttcccgg cgtattgaga
      601 gaagcattcc ggcatcctgg tcccgccgtc attgaagccg tggtcgatcc gaacgaacct
      661 ccgctgccgg gcaaagcgac cgtcgatcag gccttgacct tcgccaaggc gctggcccga
      721 ggaacgaagg atcgctgggg catcattaag acggtcatag aagacaaggt gcgggaggtc
      781 gtgtagcggt gagctcctcg aaggtcgcgc aacaggtcga tcggcgcgcc gccggagcgc
      841 agcgagtgga ggtgccggtc gatgagatca cggtctccgc ttatcatgta ccgaccgact
      901 ttcccgaatc ggacggcacg ctggcgtgga acaaaaccac gctggtattg gtggaagcga
      961 aggccgccga ccggcatggt gtcgggtaca cctatgccga caccggtacc gcccgcctga
     1021 tcaaagacac actcgcggag gtcgtcaagg ggcgcgacac gatggatgtg cccgcatgct
     1081 gggcggccat gctgcatgag atccgcaatc tcggtcggcc cggcatcgtc tccaccgctg
     1141 tttcggccgt cgatgctgcg ctgtgggatt tgaaagcccg cctattaaat cttccattag
     1201 tgagattgct cggagcggct catgcagggg cgccggtcta cggcagcggc ggctttacct
     1261 cctattcgat cgatcagctc caagagcagc tcggcggctg ggtagcggag ggcattcccc
     1321 gcgtcaagtt gaaagtcgga accgaaccgt ggaaggacgt taatcgagtc aaagcggcga
     1381 gagaggcgat cggtcccgat gtccaattgt tcgtggacgc caacggcgcg tacagccgca
     1441 aggaggcgct cgcgttcgcg ggagccttct cgtctctgaa cgtgagctgg ttcgaagaac
     1501 cggtctcctc cgacgatttg gaaggcttgc gtctcattcg cgatcgcgcg ccggccggaa
     1561 tggatatcac ggccggagaa tacggatacg acctgtttta ttttcgtcgc atgctcgagg
     1621 ccggagcggt cgatgtgctg caggccgatg cgtcccgctg cggaattacc ggctttctca
     1681 aggccgccgc cctgtgcgag gcgcgctgtc ttcccatttc agggcattgc gcgccggccc
     1741 ttcacctgca cgcggcctgc gccgccacgc cgcttcggca tctggaatat ttccacgatc
     1801 acgtccgaat cgaacagatg ctgttcgatg gagccgttaa accggaaggc ggggtgctgt
     1861 acccggacct gtccagaccc ggcatgggct tggaattcaa acgggcggac gctcaggcgt
     1921 atgccgtcta gtatccacgg aggagaatta tgctcggtca ggaattggtg caggaattgg
     1981 tgaaggacat cggacgaccg ccacggcgga cgaccaatac aacggcgcaa gtcgatgcac
     2041 aggcattggc cgccgaacta cgtaacatca taaacggcga ggttcgcttc gacgacggca
     2101 gccgcgcgct ctatgccgcc ggcggcgcca attatcgcca ggtgccgatc ggggtggtca
     2161 ttccaaagag cgtcgaagac atcatcggaa ccgtggccgc gtgccgtcgc tacggcgctc
     2221 cggtcttatc gcgcggcggc gggacgagcc tcgcgggaca gatctgcaac gtcgccgttg
     2281 tgatggacat gtccaagtac ttcaaccgga ttttggaact ggacccgcac aagagacggg
     2341 cacgggtcga gccgggccta gtcctcgacg tgctgagaaa cgccgccgag caataccatt
     2401 tgacgttcgc gccggaccct tccacgcaca accactgcac cttgggcggt atgatcggca
     2461 acaattcctg tggcgtccat gccttgatgg gaggcaagac ggaggaaaat accgaagagc
     2521 tggagattct cacctacgat ggtctgcgaa tgcgtgtcgg caagaccagc gatgatgaat
     2581 tggagcgcat tatcggagaa gcaggccgcc gcggagagat ttatcagaag ctcaagacgc
     2641 tgcgcgacaa gtatgccgat ctgatcagag agcgttatcc gaagatcccg cgtcgggtct
     2701 ccgggtacaa cctccagcaa ctcttgccgg aacacggatt cgatgttgcc aaggcgctgg
     2761 tcggcacgga agggacctgt gtgaccgtgc tggaggcaac ggtcagactc gtgtacagcc
     2821 cgccgaagcg gatcacggtt ctgctcggct accccgacgt ctatcaagcc ggcgatcatg
     2881 tgccggagat cctcgaatac cgtcccattg gattggaggg gatggatcaa gtgctcgtcg
     2941 agaatatcaa gttgaagggc acgcatccca acgacattaa gattctgccg gaagggcaag
     3001 gatggctgtt ggtggagttc ggcggcgaga cgaccgaaga agcggacgag caggcgaatc
     3061 gtatgatcag cgcgctcaag cgccatccga acgcgccgag catcaagctg tacgacgatc
     3121 cggaacacga aaagatcgtc tgggaaacca gaaaatccgg cctgggcgct accgcgcgtg
     3181 tgcccgggca acccgatacc tgggaaggtt gggaggattc ggcggtgcct ccggaaaagc
     3241 tcgggcagta tttgcgcgat ctccgcgccc tgttcgaaaa ataccgttac ggctgcgctc
     3301 tgtacggtca cttcgggcaa ggctgtgtcc acacgagaat cgacttcgac ttgaaaacga
     3361 aagacggcat caagaaattc cgttcgttca tcggtgaggc ggctgatctg gtcgtgagcc
     3421 acggcgggtc attgtcggga gaacacggag acggccagtc ccgcgctgaa tttctgccga
     3481 agatgttcgg tgatgagctc gtcgaagcgt tccgcgagtt caaaaccatc tgggatccgc
     3541 aaggcaagat gaaccctcac aagatcgtgg atccctaccg agtggacgag aaccttcgat
     3601 tgggggccga ctacaacccg cttccgacca aaacgcattt ttcatacgtt gaagatgagg
     3661 gaagcttcgc ccgagcgact ctgcgctgcg tcggcgtggg cgagtgccgc cgtgggcagc
     3721 aagggacaat gtgccccagc tatcgggtta cgcgcgagga aatgcattcg actcgagggc
     3781 gagcgcgatt gctgtgggaa atgctggagg gcaacccgct caaggacgga tggcgggagg
     3841 aggccgtcca tgatgcgttg catctatgtc tggcctgcaa ggggtgcaaa cacgattgtc
     3901 cggtgaacgt cgacatggcg acgtacaagg ccgagtttct ttcgcattac tatgccggcc
     3961 gtttgcggcc gatgcatgcc tattcgatgg gtctcattta ttggtggtcg cgtctggcct
     4021 cgctcatgcc cggtatcgcg aatttcttca cacaaacggc cggcatcagc gatgccgtca
     4081 aaactatggg agggatctcg gtccatcggc agatgccccc ctttgccagt cagactttta
     4141 cagaatggtt tcgccgccgg acgccgcaca acgtaggacg accgaaagtc atcctgtggc
     4201 cggatacgtt caataacttt ttctttccgg agacggccgt ggcggccgtc gaggtcttgg
     4261 aggcagcggg gtttcacgtt acgattccga accgttctct ctgttgcggg cggccgctgt
     4321 acgatttcgg catgctcgat ctggcgaaat ccatgtggcg gcaaatcctg gaaacgctgc
     4381 atgaggagat cgaggccggc acgcccataa tcggtttgga gccgagctgc gtggcggcct
     4441 tccgcgacga gttggttggt ttgtgtccac agagcgagga tgccaaacgg ttgaaggcgc
     4501 agacgatgac gttggcggag tttctcgaat ccaaggctcc gcacatcgaa ttgcctcagc
     4561 tgcctcggaa ggcgatcgtt cacggacatt gccatcaaaa ggcgatcatg gggatgagcg
     4621 ctgagcaaaa ggtgctcaag cgcatggggc tggattttca gattctcgat tcaggatgct
     4681 gcggtatggc cggctcgttc ggattcaagg acggcgacca ctacgacgtg tcgataaagg
     4741 tcggtgagct ggtgctgctg cccgaggtac gcaaggcacc gaaagagacc ttgattctcg
     4801 ccgacggctt cagttgtcgt gagcagatct tgcagaccac cgaccggcgc ggcctgcatc
     4861 ttgcccaagt gctgcagatg gcgcttcgtg acgggcaacg ggtcggcccc ggcgtgcctg
     4921 ggtgggagta tcccgagcgc cgttacgtca acccgcccgc ccttcacgcg ccctttacac
     4981 gaaaagacgc ggcggtgctc tttggagcag gcgccttgct gctcggcggc gcggcggcat
     5041 ggctgttgag tcgacgaagg cattttaggt gagaagagcg caccatgggc gggcgtcggt
     5101 tttacgagat gctgaacaac gccgggtgtg cagggcgcac caaggaagcg aaaggcgatt
     5161 ggccgctctt gttcgccggt ggagcatctg cttccgacat gaacaaacgt tgccttatgg
     5221 gatgtgccgt cgatcggcag cctatttctt ccgcaatcca aaaaataccc cggcaccgcc
     5281 ggcgccggct gcaggaagat agccggtcag cacttccttg aaaccctggg ctttgcctcg
     5341 ggcccagtca agcgtacgat tcacatccgc ttggatcaag gtccaatcca ggtgaatcca
     5401 gccggtcgtt ttaaggatgg caatgagtgc cagcaatgag atggtgacca acagggcgag
     5461 ctttagggtt ttccggaatg cccatccaag caagaatccc ccgaggaagc tgccacccgc
     5521 gctcgcgagt gccggcgaca tcatattact gagccaggcg ccgagtccgg cgacagtcgt
     5581 cgcaccggcg gcgagaaacg atttagccgc ccagggaggg tcggacatca ttgtccccag
     5641 tgtcttagca aggaagccgc tttgaggtgg aggaactgga ttgctcatag ccggtttgtc
     5701 tcgaatgtgt tacagaccga gggatcgccg gattcaaggc cgcgcttaag ccaggtcatc
     5761 cgttgttcag atgagccgtg ggtccagctt tccggttgaa catggccacg cgacatcttc
     5821 tgtaagcgat catctccgat ggctgctgcc gcgcgcagcc cttcttcgaa atcgcccggc
     5881 tcgatcaagt ttcgctcgcg atcggcatga tgaccccaga caccggcata acaatcggct
     5941 tggagttcca tccggactga aagggcgttt ccttccacct cagaaacctg tcgttggagg
     6001 cgatgcactt tttctgcgac gccaaggaga ttttgcacgt gatggccgat ctcatgggcg
     6061 atcacatagg cttgagcaaa gtcacccgcc gctccaagac gatgcgccat ctcatcgaag
     6121 aaggcaagat ccaaatagac ccggtgatcg ttggggcaat agaacggtcc aacagccgac
     6181 gaggtggtgc cgcatgccga ttgcacggca ccggaaaaga ggacaagccg tgggtccttg
     6241 taccgaggac cgaggaggct tcgccaggtc gtttcggtgt ctgcaaggac gacggaggca
     6301 aacttgccga gtcggtcttg agggacgccg acaggaccgg gttcagatgg ggcggacacc
     6361 tcggtcatgc tctgaacacc ggtgaggaga ttcagcacgg tgaggggatt gacgcccgtg
     6421 aaatagctga cggcgagtgc caacacaatg gtgccgatgc cgaggccgcc tttcccgagg
     6481 cgggccggac ccatgccgcg acgatcttcg atgttgtcgc tttcacgttg gtcgtcaatg
     6541 cgcataggcg ctctccctca cgctccacac tcagtccgat tccttctcca tcgactggat
     6601 gaacttgtcg gcttcggcga tggaacggtt catgtctttg accagctgat cgacctgcgc
     6661 gtcgactttg acgagttcgc ccttaatggc ggcgagtgcg cgtgcgttca ggttgtgttt
     6721 caagtagagc acctgatctc gcaatggttt aaggaccggt tcaatccgct gctcggcccg
     6781 tttcatggcc ccgagcatct ctctgtagcg acccttggtc tgggtgagtt tggcttggct
     6841 tttccggcgc aggtcggcgc tcgagtattg accaagctct tcttcccatt ccgaaaagag
     6901 tgcatcggcc acactttcga catccgagat tcgtttccgg accgatttgg cactgtcctc
     6961 gcttgactcg agttcaccgc tcagcttctt ataggtgtct tccagatctc ctccttcata
     7021 ggagaccacc ttgccgaatt gctccagcgt gctctgaatc tctttcttcg cgtcttcttg
     7081 ggcatcccgc gccgatttga cgcggctgct caggatatcg cgtttggcat agcccatttt
     7141 ctccatggtg gccatatagg ccttgtcaca agaggacagg ccaaatgaca tgaacagcag
     7201 tatgacagct agaatcaccc gcaacatgat gacctctact ctcggatgtg ggctcgacga
     7261 gcggcgaccc tgagacgcca cgatgggacg ttgcatcata actgaagagc atacaggggg
     7321 aaagccccta ggtcggcgct ctcacgagcc ctggacgagt agaatcggca tcgctatcgc
     7381 gctatagtat gaacatgcgt ctcacatcct tcaacgtgtg gtggtgggcg gtgttggtgc
     7441 tggcgacagc ctgttcctca caacgagtga ttcctgaaga gctggagcct ctggtcgacc
     7501 gaacggttcc gtttcctgag gtggtggccg ctcctgattc ataccaaggc aagatcgtgg
     7561 tattcggtgg cgaggtgctg aaggcgaaac gactgaaaga tggaacacag atcgagcttc
     7621 tgcagttgcc cctcgataag gacgagagac cgattctcga ccgtcaacaa tcgcagggac
     7681 ggttcctcgc ggttcatcag gagtttctcg atcccgcaac cattgccgag ggaacaaaaa
     7741 tgacgatcgt tggagaactg tcgaaggcaa aggtcgagca cctcgacgat gtcgaatatc
     7801 gataccctgt gttgatcgtg aaatatctgc acacgtggcc ggcgaaatcc tatggctctg
     7861 cctacccgca ccctcgattt tcaattggaa tcggaggcgg aacgggcggc agattcggcg
     7921 gcggcggcgg gttcggcatt ggattttgat tcgtgaagag gcatgggcaa gaaatgtcat
     7981 ccgcgtagtt ccgcagctgc tgcattttcg gtcagtctta ctgacacctc gataacattg
     8041 ctctacgccc ttgatttcta tcgacaaacc atggagccgc ttgcgcgagc tgtcttcgcg
     8101 tcagacattc aagacggggt cgcccgtgga gtcacttgcg tgtacggaat ggagcatgat
     8161 atgcaagtat aagccg
//
LOCUS       DATMTI010000600         7880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_659031, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000600 DATMTI010000000
VERSION     DATMTI010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659031"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..682
                     /locus_tag="VN039_09550"
     CDS             416..682
                     /locus_tag="VN039_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWV46247.1"
                     /translation="MPNITLLVSPSCGACPSAKSLWKQLRVKYSFSYREVDITTKDGQ
                     ELADRHSVRAVPATIIDGRLTFVGVPSRESAEKAIQLKIKQREE"
     gene            690..842
                     /locus_tag="VN039_09555"
     CDS             690..842
                     /locus_tag="VN039_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46248.1"
                     /translation="MDEDDIELPVLPKLDRPPPDCPICGDPMSFIDGDWACVDCNGEL
                     LGPETG"
     gene            859..4029
                     /locus_tag="VN039_09560"
     CDS             859..4029
                     /locus_tag="VN039_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWV46249.1"
                     /translation="MLRVITGRFHPDLESALVDHLTRVKATDPLAAIAIFVPSKPLAD
                     RIRTLLAVEHRLSLLNLHILTFHQLALRLASEVPPAHSLPRVVDELFFEQLVRHIVRS
                     KLSSLAPLQRIGQSAGTWGALWATIRDLKDADVDPVQALQGLREGYFEEDDREWLGAL
                     ISLYAAVKEGGKTLGVGTRDDLAEALIPFVPTASFFQSLSEVFYYGFYDLTQVQLSLF
                     EAISRAKETMLFFPLEDDPAYGFAGRFFDRYIRPLMMPDEAMIRLERKSSIVAPTPVQ
                     LTVRSVIGAEEELATTCRTILDLVETHGYRFDEIGVIARTLDPYSPHLRLIFDRHRIP
                     FCTTASQPLIQEPVCKLVLQLASLPLNDLYRTSVLDVVTSPLYVIDLHDELSESYRPE
                     QWKLLVQALHITHGVEEWKRLEQASRASLILDDEESLVGSPAIAPEVIKLLWQVVSQL
                     TQDCAALPSRGTIDRLVTACRALVDRRLRRPETAASNAEEVQLARQAMTWDAIDRIWV
                     TLTELEPLNEEMTWAEFVELLTHTFERTTKPLHDSSPQGVMVCDVMAARGVPFKALFV
                     LGLNEKVFPRYIREDAFLRDSHRLVLDATLGFKIDEKLTAYGEETLLFHLCRQAASQR
                     LYLSYQRADESGRMLAVSPYLGEARRRCGHGEQPIDMVPRRLTDRVLQRPTIKLFLSP
                     AELTQWSAINGQDPTDLVQAFGRDADTFRHAATALDRIEEDGSLLNLFDGITGTVESH
                     WARIIERGLAPTPLERYARCPFQYFAADVLRLEPSRVAIKQEPEVALVGTLCHATLRR
                     CYEQLVQAGWPTAPMADNMVDQTVRASVEQAAADLESQHRTGHYLLWELAKEQIAALV
                     IAAVKADETEQAEHPYQPIAFEVDGEGTVPGVLEEGLLKIRGRIDRLDRNRLSGALRV
                     VDYKFKIGSSMKPEDRNLVQSAVRGYRLQPPLYSCLSVPGQPTPNLVQFLFLAPQWST
                     TISRSTFETMSWSSDTGARIQNTIRTLVDGVRTGRFFILPDGYCDGCDFRVVCRREHA
                     PTWWRAHRATEPKTLKMLRAQKVADE"
     gene            4022..7366
                     /locus_tag="VN039_09565"
     CDS             4022..7366
                     /locus_tag="VN039_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWV46250.1"
                     /translation="MNDDLLISDRMARESAETTFDRNVVVVAGAGTGKTTLLVNRLVY
                     LLMKEPNPVPITQVVALTFTNKAATEMKVRLRERLAVLARPEADSVQSSDGGAVSCSD
                     LQARYRLSVDAIAARAQAAFNDIEKAQIGTLHSFAAHLLRLHPLESGVDPDFKEDDGL
                     RFEEQFAAAWDCWLDQELSRAGQHHHLWRSVLSLTTLDAVRVLSRSLCSELVDLNTLQ
                     QQLASIAVTPAVSSWIQHMGDRANQLMKTYDRPKRRKVEHMLAAASSLMRLVAAKGLP
                     GRQDLTTAERGWLDKDLGNAVGEWEKGDFKEAAALIQTAQHLQSVDHGFLNDLLTLLL
                     PLVRNIRETFSRQGWLSFDGLLARARTLLFEHPSVRERIKRDYRAVLVDEFQDTDPVQ
                     YEIILAVSERQGSQAVDWRDMLLEPGKLFIVGDPKQSIYAFRRADIEAFDRVMEKVTA
                     DGGLERTLTTNFRSDAAVLEPVNEVFDRLFERQPLVQPANVRLEVRPQRRPASIEPGV
                     RLNVTVPDGEEAFDAAGATRAESEVLARWLADEVLSHSSMKPGHVALLFRKLTQADSY
                     LDALRRHDIPYVIEGEKHFYRRQEVIDLANLLRVLDHPHDEIALTGLLRSPLGGLTDR
                     ELYELKQAGHFNYLRAARLEGWSHARAAAVRRLYEHLAWLHRAIPVLPLADAIELLFD
                     RLPILDLAAASLHGEQAVANLMKVKQTAASLADRPHMTLSGFVELMIARLEEQPDESE
                     SPLAEESLEAVHVLTIHKAKGLEFPIVVLPGLHQGSGRERSVPQVSYDWSSGTYGLSL
                     DRHWSLGSLLVRHKLRLREEAERRRVLYVGMTRAKELLLLSGGITTRSVGETVFDLLG
                     NIGTGEIGTASTEALKVGGSEIPHRVIRAPERKRPRRRVDRITNAALIDPKEIAERWE
                     IRTARWTEIRETPHHLTPTTLGKRAASVIREPIPVRQDAEIGRLAGVVAHRLLERWDF
                     SHDPSELFVQVVLAVQATLGPDDQSRAGAVVESVHDLLATFSRSEAYARLRSSKILGR
                     EVPFIMPWEDRQVMEGVIDLIYRLNGELWIADYKTDAIAADEAVLRAERYRMQSHVYK
                     AAVKQSLRIEPRFHCLFLRCAMTVEL"
     gene            7385..7762
                     /locus_tag="VN039_09570"
     CDS             7385..7762
                     /locus_tag="VN039_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWV46251.1"
                     /translation="MAKRDHISSEAFAQLVQQAIADLPPPYAKLMESIAVVVEEEPPE
                     EVLRDLDLDRRDDLLGLYQGRSLAADSFFGSGGEPPPQISIYRGPILRLCESPEEVVQ
                     EVYDTVVHELGHHVGLDDDEMPY"
BASE COUNT         1625 a         2161 c         2383 g         1711 t
ORIGIN      
        1 gaagttagag gtgatcgtat ggactccttg tcaaaatgcg gctgcggacg attatccggt
       61 ttcctttgtt gcgtaatgct gatggcatgt atgggttgca taccgaagga aactctaaac
      121 gctctatccc agaataccgg agagccggga agcaccatcc ctcgatccca cgctccctat
      181 tatgctaata gtgccgggaa cattgttgcc ccgccgggaa atccagctgg aaatcccgcc
      241 ggctctccgc ccatcatcaa tggcacggaa aattcgtccg acggatggcc gtcagggaga
      301 taaattctct cgcgattatc cgatccaacg gcgtgtcatt gggcaagcgc tccttgcgcc
      361 ggcttttcag ccgatgttaa gatcgaacat gaaaggctga gggacttgtc cgacgatgcc
      421 gaacatcacc ttgcttgtgt cgcccagttg cggagcctgt ccgtcagcca agagtctgtg
      481 gaagcagctg agagttaaat acagcttctc ttatcgggaa gttgatatca cgaccaaaga
      541 cggtcaggaa ctggccgatc ggcattctgt ccgtgccgtg cctgcaacca tcattgacgg
      601 acggttgacg tttgtcggag tgccgagtcg agagagtgcg gaaaaggcca ttcaattgaa
      661 aattaaacaa cgggaagagt agggagtcca tggacgaaga cgatatcgaa ctaccggtcc
      721 ttcctaaact cgatagacct cctcccgatt gtccgatctg cggcgacccc atgtctttca
      781 tcgacggcga ttgggcctgt gtcgattgca acggggagct cttggggccg gagaccgggt
      841 agcgtacaat gacccgctat gttgcgggtc atcaccggtc ggtttcatcc tgatcttgag
      901 tctgctctcg tcgatcacct cacgcgcgtc aaggcaaccg atccgctcgc ggccatcgcg
      961 atcttcgttc cgtccaagcc gttagccgat cgtattcgca ccctgcttgc cgtcgaacac
     1021 cggctgtcgc tcctgaacct acacatcttg accttccatc aattggccct gcgtctggct
     1081 agtgaagtac ctcctgctca ctcgctgccg cgagtcgtcg acgaactgtt cttcgaacaa
     1141 ttggtccgtc acattgttcg cagtaagctc tccagcctgg ctccattaca acgaatcggg
     1201 caatccgctg ggacgtgggg ggcactctgg gcgacgattc gtgacttgaa agatgccgat
     1261 gtcgatccag tgcaagccct gcaaggcctc cgtgaaggct atttcgaaga ggacgacaga
     1321 gagtggctcg gcgcgctcat ttctctctac gcggcagtga aggaaggggg taagacgctc
     1381 ggggttggaa cgcgagacga tctggctgag gcgctcatcc cctttgtccc gacggcctcg
     1441 ttcttccaat cgttgagcga agtgttttat tacgggttct atgacctgac gcaggtgcag
     1501 ctgtctctct ttgaagccat cagccgagcg aaggagacaa tgctgttttt tccgctggag
     1561 gatgatccgg cctatggatt cgccgggcgt tttttcgatc gatacatccg gcctctgatg
     1621 atgccggatg aggcgatgat ccgattggaa cggaaatcgt ctatcgtggc tccgacccct
     1681 gtccaactca ccgtgaggag tgtgatcggc gcggaagaag aattggcgac aacttgccgc
     1741 acgatcctcg atctggtcga aacgcacggc tatcgattcg acgagatcgg cgtcattgcc
     1801 cgtacgcttg atccctacag cccgcatctc cggttgatct tcgatcgcca tcgcatcccc
     1861 ttttgtacga ccgcgtcgca gccgttgatt caggaaccgg tctgcaaact cgtcttgcag
     1921 ctggcctccc tgccgttgaa cgatttgtat cgcacgtcgg tcctcgatgt cgtgacgtct
     1981 ccgctctatg tcatcgattt gcatgacgag ctgtcggagt cataccggcc cgaacagtgg
     2041 aagctgctcg tccaagccct gcacatcact cacggagttg aggagtggaa acgattggaa
     2101 caggcgagcc gagcttcatt gattctcgat gacgaggaga gtctcgtcgg ttctccggcc
     2161 atagcgccgg aggtaattaa attgctctgg caggtggtct cacagcttac gcaagactgt
     2221 gcggcccttc catcgcgagg tacgatcgac cggctggtca cggcttgtcg agcactcgtc
     2281 gatcggcgtc tgcgtcggcc cgagactgcc gcatcgaatg ccgaggaagt tcagctcgcc
     2341 cgccaagcga tgacatggga cgccatcgat cgaatatggg tgaccctcac ggaattggag
     2401 ccgctcaatg aggagatgac gtgggctgaa tttgtcgaat tgctgacgca taccttcgag
     2461 cggacgacga agcccctaca cgatagctca ccacagggag tgatggtgtg cgacgtcatg
     2521 gcggctcgcg gggtgccgtt caaagccctg ttcgtcctcg gtttgaacga aaaagtattt
     2581 ccgcgttata tccgagagga cgcgtttctg cgggatagcc atcggctggt gctcgacgcc
     2641 acgctcggat tcaagatcga cgagaagctg acagcgtatg gagaagagac gttgctcttt
     2701 cacctgtgcc gtcaggctgc ctctcagcgg ctgtacctat cctatcaacg ggccgatgaa
     2761 tcggggcgca tgctggccgt ctctccctat ctcggagaag cccgccgccg gtgcggccat
     2821 ggtgaacaac cgatagatat ggtgccgcgc cgcctgacgg atcgggtctt gcagcggccc
     2881 acgatcaaac tgtttctgtc accggctgaa ctgactcagt ggtcggccat caatggacaa
     2941 gatccgaccg atctcgtgca ggcgttcggg cgtgatgcgg atacattccg ccatgcggca
     3001 acggcgctcg atcgaatcga agaggacggc tcactgttga atcttttcga tgggatcaca
     3061 gggacggtcg aatctcattg ggcgcggatt atcgagcggg gtctcgctcc gacgccgctc
     3121 gaacggtatg cgcgttgtcc gtttcaatat tttgccgctg atgtgctgcg actcgaaccg
     3181 agccgagttg cgattaaaca ggaaccggag gtggcgttgg tcggtacgct ctgccacgcg
     3241 acgttacgtc gttgctatga acagcttgta caggccgggt ggccgaccgc accaatggcg
     3301 gacaatatgg tggatcagac ggtccgtgcg tcggtggaac aggcggcggc agacttggag
     3361 tcacaacatc ggacaggcca ctatctgctg tgggagttgg cgaaagaaca gattgcggca
     3421 ctggtgattg cggcggtgaa ggcggatgaa acggaacagg ccgagcatcc ctaccagcct
     3481 attgcctttg aagtggacgg agaaggaacg gttcccggcg tcctggagga gggattgttg
     3541 aagatccgtg gtcgcatcga tcggctcgac cggaaccggc tctccggcgc attacgcgtg
     3601 gtcgattaca agtttaagat cggctcatcc atgaaaccgg aagaccgtaa tctggtccag
     3661 tcggcggtcc gtgggtatcg gctgcaaccg ccgctctaca gttgcctcag cgttcccgga
     3721 caacccacac cgaacctggt gcaattcctg tttctcgccc ctcagtggtc gacaacaatc
     3781 agtcgctcaa cgtttgagac gatgtcatgg tcatccgata ccggtgcacg cattcagaac
     3841 acgatcagaa ccttggtgga cggtgtacgt accggacgat ttttcattct tccggacggc
     3901 tattgcgacg gatgcgactt tcgtgtggtc tgccggcgag aacatgctcc gacttggtgg
     3961 agagctcatc gtgcgaccga acccaaaacg ctgaaaatgc ttcgcgcgca gaaggttgcc
     4021 gatgaatgat gatctcctga tatccgatcg catggcgcgg gagtcggctg agacgacgtt
     4081 cgatcgcaac gtggtcgtcg tggccggggc ggggacgggc aagacgacct tgcttgtgaa
     4141 tcgtctcgtg tatctgctca tgaaagagcc caatccggtc ccgattacgc aggtcgtcgc
     4201 cttgacgttc actaacaagg cggcgaccga aatgaaagtg cgactgcgcg agcggcttgc
     4261 cgttctcgcc cgtccggagg ccgattcggt gcaatcgagc gatggggggg ctgtctcgtg
     4321 cagcgacctt caagcacgtt ataggctgag cgtcgacgcg attgccgcac gggctcaggc
     4381 ggcattcaac gacattgaaa aggcccaaat cggcaccctc cacagtttcg ctgcacactt
     4441 gctgcggctt catccgctgg agagcggagt cgatcctgat ttcaaagagg acgacggctt
     4501 gcgatttgaa gagcagttcg ccgctgcgtg ggattgctgg ctcgaccagg aattgagccg
     4561 agccggacaa catcatcacc tgtggcgatc ggtcttatcg ctgaccactc tcgatgcggt
     4621 cagagtcttg agccggtccc tgtgcagcga attggtcgat ctcaacacgc tgcaacaaca
     4681 acttgcgtcg atagcggtga ctccggccgt ctcgagctgg attcagcaca tgggagaccg
     4741 ggcgaatcag ctcatgaaaa cgtatgacag gccgaagcgc cgcaaggtcg agcacatgct
     4801 cgccgcggcg tcgtctttga tgcggctcgt ggcggcaaaa gggttgccgg gccggcaaga
     4861 cttgacgacg gcagaacgag gatggctcga caaggatctc ggaaacgctg tgggtgaatg
     4921 ggagaagggc gatttcaaag aggccgctgc gctcatccaa acggcgcagc atctacagtc
     4981 ggtcgatcat ggattcttga acgatctctt gaccctgctc cttccgttgg tgcggaacat
     5041 tcgcgaaacc ttttctcggc aaggctggct ttcgtttgat gggttgctgg cacgggcacg
     5101 gacgctcctc ttcgaacatc cgtcagtccg tgaacggatc aagcgggact atcgagccgt
     5161 gctggtcgat gagtttcagg acacggaccc ggtgcagtac gaaatcatcc ttgcggtctc
     5221 ggagcggcaa gggagccagg cggtcgactg gcgagacatg ctcctcgaac cggggaaact
     5281 ctttattgtc ggagatccca agcagtcgat ctatgccttt cgccgcgccg acatcgaagc
     5341 ctttgatcgg gttatggaga aagtcacggc tgacggtggc ctggaacgaa cgttgacgac
     5401 gaactttcga agcgacgcgg ccgtcctgga gccggtgaat gaggtcttcg atcggctgtt
     5461 cgagcgacag ccgctggtcc agccggcgaa tgtccgcctg gaggtgcgcc ctcaacgacg
     5521 ccccgcgtcg atcgaaccgg gcgttcgtct caacgtgacg gtgccggatg gtgaagaggc
     5581 attcgatgca gcaggggcga cgagagccga gagcgaagtg ttggctcgtt ggctggccga
     5641 cgaggtcttg agtcattctt caatgaagcc gggccacgtg gcgttgctgt ttagaaagct
     5701 cacgcaagcc gactcctatc tcgatgccct gcgccggcat gacattccct acgtcatcga
     5761 aggggaaaag catttctatc gccgccaaga agtgatcgac ctcgccaatc tgctgcgggt
     5821 cttggatcat cctcatgacg agatcgcctt gaccggcctc ctccgttcac cgcttggggg
     5881 tctgacggat cgggaactct atgagcttaa gcaggcgggg catttcaatt atctccgcgc
     5941 ggcgcgtcta gagggatggt cccatgcccg tgccgctgct gttcggcggc tgtacgaaca
     6001 cctggcttgg ctccatcggg ccatccctgt gctgccattg gccgatgcca tcgagctgct
     6061 atttgaccga ttgcccattc tggacttggc tgctgcatca ctccatggcg aacaggccgt
     6121 ggcgaatctc atgaaggtga aacagacggc tgcgtcattg gccgaccggc cccacatgac
     6181 cttgagtggg tttgtggaac tcatgatcgc ccgacttgaa gaacaaccgg acgaatcgga
     6241 gagtcctctc gccgaggagt ccctcgaagc cgtgcatgtc ttaacgatcc ataaggccaa
     6301 ggggttggaa tttcccattg ttgtgctgcc gggcctccat caggggagcg gacgagagcg
     6361 cagtgtgccg caggtctctt atgactggtc gagcggaacc tacgggcttt ccctggaccg
     6421 tcactggtcc ctcggctcgc tgctggttcg acacaagctg cggctgcgag aagaggcgga
     6481 acggcgacgt gtgctctatg tggggatgac cagagccaaa gagttgttgc tattgtctgg
     6541 ggggatcacc actcgctccg tcggtgaaac ggttttcgac ctgctgggga acatcggtac
     6601 gggggagatc gggacagcct cgaccgaggc cttgaaggtc ggcggcagcg agattcctca
     6661 tcgagtgatc agagcaccgg aacgaaagcg gcctcgccga cgagtggaca gaataaccaa
     6721 tgcggcattg attgatccga aagagattgc cgagcggtgg gaaataagga ccgctcgatg
     6781 gacggagatt cgtgagaccc cgcatcatct gacgcccacg acgctgggca aacgagcggc
     6841 gtccgtcata cgtgagccga ttccggttcg acaggatgcg gagattggac ggttagctgg
     6901 tgtagtggct catcgtctgc ttgaacgatg ggacttttca catgatcctt ccgaattatt
     6961 tgttcaggtt gtcttagccg ttcaggcaac tcttggaccg gatgaccagt ctcgtgccgg
     7021 agcggtcgtt gaatccgtgc acgatctcct tgcaactttc agtcgatcgg aagcctatgc
     7081 acgcctccgg tcatccaaga tccttggccg tgaagttccg ttcatcatgc cgtgggaaga
     7141 tcggcaggtc atggaaggag tcatcgacct catctaccgg ttgaatggag agctgtggat
     7201 cgccgactat aagacagatg ctatcgcggc cgatgaagca gtgcttcgtg cagagcggta
     7261 ccgaatgcaa agccacgtgt acaaggccgc tgtaaaacag agtctgagaa ttgaaccgcg
     7321 gtttcactgt ctgttccttc gttgtgcgat gacggtagaa ttataagggt aggcgataat
     7381 ggagatggct aagcgagacc atatctcatc tgaagccttt gcacagctgg ttcaacaggc
     7441 gatcgctgat ctgcctcccc cttatgccaa gctgatggaa tcgatcgccg tcgtggtgga
     7501 ggaagaaccg ccggaagagg tgttgcggga cctggatctg gacaggaggg acgacttgct
     7561 cggcctgtat cagggccggt ctctggcggc tgattcgttc ttcgggtcgg gaggagaacc
     7621 accgcctcag atttccatct atcgtggacc gatcctccga ttgtgtgaga gtccggagga
     7681 ggtggtgcag gaagtgtacg ataccgtcgt tcacgaactg ggccaccatg tcggcctgga
     7741 cgatgacgag atgccgtact gaattcagtg ctgagaatcg agtgctcgcc tcgctgagtc
     7801 gaagcgggct gtatgctgag ttgaaaacgc ccggaaactc cgctcagcac tcaggattca
     7861 gcactcatca ctgtcaagag
//
LOCUS       DATMTI010000601         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_489733, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000601 DATMTI010000000
VERSION     DATMTI010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489733"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VN039_09575"
     CDS             <1..1657
                     /locus_tag="VN039_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HWV46252.1"
                     /translation="DTPFEEALQLILNSNSLFSRPISPAVLIVSPDTKQKQEQYRDLM
                     IRTFYLSTAKAKDMVLLLKSMLDSKRMHANEQLNAIVIRDQPEKLELAEKIILANDRQ
                     EPEVLFDLEVLEVNRTKNQTYGLNYPKQVGAGLIASGFTGTLAAEAVQMTYRQLTDLG
                     PSNYLFKLPTTVLLDFFKQQSDAKTLASPKVRVMNNKKAEINIGDKQPILLSTTNVLP
                     GQAATGAVPTTSTVTSIEFRDTGVKLTVEPSIRLGNELSLKMKVEVIRVGDQITLQAS
                     PLIQQFKFGNRSAETTLSMRDGETIVLGGLIQEEDRRTRVTMPWVGDLPLIGKLLSSF
                     KTERITTEVILTITPRIAQPALSTGSSHHAFWSGTEFNYATSPVFSNPSRKASALVGR
                     EYGKIPAHGGAMTKDAGQTNMVRSLAQLATPDPLLVIKPEESVVQSGKELRLSVVDGR
                     ISSSGDPTFKLGYDHEILQFKRLDNAELIDTGETTAVDKVGQAGAITFHFARPAQRSP
                     RTVNVIFVAKAPGVSPIRVELTDSDSAAQASPEVVGTGVVRVR"
BASE COUNT          447 a          462 c          496 g          403 t
ORIGIN      
        1 ggacacgcct tttgaagaag cgctgcaact gattctcaat agtaacagtt tattttctcg
       61 tccgatatct ccagctgtct tgatcgtcag tcccgacacc aagcagaagc aggagcagta
      121 tcgagatcta atgattcgaa cattttatct ttcgacggcg aaggccaaag atatggtgct
      181 cttgctgaag agcatgttgg actccaagcg aatgcatgcc aacgaacaac tcaatgcgat
      241 cgtcattcgc gatcagccgg agaaattgga acttgccgaa aagatcatcc ttgccaatga
      301 ccggcaggag ccggaggttc tctttgatct tgaggtattg gaggttaatc ggacgaagaa
      361 tcaaacgtat ggcttgaact atccgaaaca ggtcggtgct gggctgatcg cttccgggtt
      421 tacgggaact ctggctgcag aggctgtgca gatgacctac cgccaactga cggatcttgg
      481 gccgagcaac tatctcttta agctgccgac gacagtgttg ctggatttct tcaaacaaca
      541 atctgatgcc aagaccctcg cgtcaccaaa agttcgcgtg atgaataaca agaaagcgga
      601 gattaatatc ggggataaac aaccgattct tctctccacc accaatgtct taccggggca
      661 agctgcgaca ggagcagtac cgaccacctc aaccgtcaca tcgatcgagt tccgagatac
      721 aggcgtcaaa ctgactgtcg agccttcaat ccgattgggc aatgaattgt cgttgaagat
      781 gaaggtagag gtgattcggg tcggagatca gatcacgcta caggcctctc cgctcattca
      841 acagtttaaa ttcggtaatc gctcggcaga gacaacgctg agtatgcggg atggagaaac
      901 aatcgtcctc ggcgggctga ttcaggaaga ggatcgtcga acgcgggtca ccatgccatg
      961 ggttggggac ttgccgctga tcgggaaatt gttaagctcg ttcaaaacgg aacggattac
     1021 gacggaggtc attctgacca ttacgccacg catcgcgcaa ccggctcttt ctactggctc
     1081 cagccatcac gcattctggt ctggtaccga attcaactat gcgacaagcc cggtcttttc
     1141 caatccctct aggaaggcat cggccttggt gggccgcgag tatggaaaga ttcctgccca
     1201 tggtggcgct atgacgaaag acgctggaca gacaaacatg gtccgatcat tggcccagct
     1261 cgcaacgccc gatcctctgt tggtgatcaa gccggaagaa tcggttgttc aatcaggcaa
     1321 agaactacga ctttctgttg tagacgggcg aatcagttca tcgggcgacc ccacattcaa
     1381 actgggctat gaccacgaga ttctgcaatt caagcgactt gataatgcgg agttgatcga
     1441 cacgggcgag accacagcgg tggacaaggt cgggcaagca ggagccatca cgttccattt
     1501 tgctcgcccg gctcagcgtt cgccgcgaac cgtgaacgtc atctttgtgg cgaaggcgcc
     1561 aggagtgtcg ccgattcgcg ttgaactgac cgattctgac agtgctgcgc aggcatcgcc
     1621 agaagtagtc gggacagggg tggtgcgcgt gcggtgaggg aggacggcct aacactcatc
     1681 gagattctcg tcaccatggc cattattgtg atcctggctt cggtcgccat acctctgagt
     1741 aaggtgtcga caaagcgagc tcaggagatt gaactgcgcc agcatcttcg gtcgattcgc
     1801 ggcgcgat
//
LOCUS       DATMTI010000602         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_404624, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000602 DATMTI010000000
VERSION     DATMTI010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404624"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VN039_09580"
     CDS             <1..263
                     /locus_tag="VN039_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46253.1"
                     /translation="WLFPGKDAVGREGWFLRIEVTGMWPRRCGPFATQDKACEFLEGF
                     LHRHVLGGFCEIQSEMTPPHQTCVTEGVPQLAATPNGAGGSR"
     gene            478..918
                     /locus_tag="VN039_09585"
     CDS             478..918
                     /locus_tag="VN039_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46254.1"
                     /translation="MKKSKQAKEQITPRSMLQIRVVRQDEKGTIQDVTHEDDRGGYGL
                     TGIQMRSSCAYDSLLRTRELLGLLELAVENRDHVDAALVRRVLGDVVTELSRGGDLGC
                     YVQYLQELFEGTMALPRITGITPRVFDKSYLSPRKTDHSLALVS"
     gene            950..1828
                     /locus_tag="VN039_09590"
     CDS             950..1828
                     /locus_tag="VN039_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46255.1"
                     /translation="MGMLYKRGKVFWIKYYLAGRPIRESTGTTKQKEAERFLKDREGR
                     VAMGTPALPRIDRIRYDELADDLRRHYEVTNCRDLKEADVRLKPLKAFFSGRRASGIG
                     GPDIERYVQVRQGAAVSNGAINRELSTLGRMLRLAYEHGKLQRVPMIHRLKEAAPRQG
                     FFEREQFAAVRKQLPPDLQAAVTIAYTYGWRMQSEVLALTLSQLDLEAGTLRLEPGTT
                     KNAEGRLVYLTPELQALMSAQVDRVKALARQLNRLTPLSLSARDRQVSRETPPGLSQG
                     LAHRLGTGGACGDVAP"
     gene            2050..2125
                     /locus_tag="VN039_09595"
     tRNA            2050..2125
                     /locus_tag="VN039_09595"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Met,seq:cat)
BASE COUNT          526 a          620 c          677 g          471 t
ORIGIN      
        1 actggctgtt tccgggcaag gatgcggttg ggcgcgaggg gtggtttctc cgcattgagg
       61 tcaccggcat gtggccgcga cgatgcgggc cattcgcgac acaagacaag gcgtgtgaat
      121 ttctagaggg gttcctgcac aggcacgtgc tgggaggctt ctgcgaaatc caaagcgaga
      181 tgaccccacc tcatcaaacg tgtgtgaccg aaggcgtacc tcagttagct gcgacgccca
      241 atggagcggg gggctcgcga tgaacatgaa gcaatttcgc agaccggcca atggacactc
      301 ccgtacggcg atcgcaggcg gacgagtggt tggcggacgc ggctttcggt tgcaatcgaa
      361 actgggccga tccatcaccc tagatcggct ggagtatctc ctctcccaga cccgtaagcc
      421 gaacataagc agacaaggcg cataaccatt caccttcata aaaagggaga ctccgacatg
      481 aaaaagagta agcaggctaa agagcagatc acacctcgaa gcatgctgca gatccgggtc
      541 gtgcgtcagg atgagaaggg caccattcag gatgtgacgc acgaggacga tcgcggcgga
      601 tatgggctga ctggtatcca gatgagaagc tcatgtgcat acgacagcct cctacgcacg
      661 agagaacttc tagggttgct cgaactcgca gtggagaaca gggaccatgt ggatgctgcg
      721 ctggtgcggc gcgtactggg cgatgtggtg acagagttat ctcgcggtgg cgatcttgga
      781 tgctatgtgc agtatctgca agaactcttc gagggcacga tggcgttacc tcggatcact
      841 gggattacgc cgcgagtgtt cgacaagagc tatttgtcac cgaggaagac ggatcactcg
      901 ctggctcttg tgtcgtgaac catacaataa gccaaaagga gcacacagta tgggcatgct
      961 gtacaaacgc gggaaggtgt tctggatcaa atactatttg gcagggcgtc cgattcggga
     1021 gagtacgggg acgaccaaac agaaggaggc tgaacggttc ttgaaagacc gtgaaggccg
     1081 ggtcgcaatg gggacccctg ccctgccacg gattgaccgc atccgctacg atgaactggc
     1141 ggatgatctt cgccgccatt atgaagtgac taactgccga gacctgaaag aggcagacgt
     1201 tcgactcaag ccgctcaagg cattctttag cgggcgccga gcctcaggaa ttggcggacc
     1261 ggacattgag cgctatgttc aagttcggca aggtgcggca gtcagtaacg gggccattaa
     1321 tcgggaactc tccacattgg gacgcatgtt acggttagcc tacgagcatg gaaaactgca
     1381 gcgtgtacca atgattcatc gcctgaaaga agctgctcct cgtcaaggtt tctttgagcg
     1441 agagcagttt gcagccgttc gaaagcaact tcccccagat ctgcaagcgg cggtcacgat
     1501 tgcgtatacc tacggctggc gcatgcagag cgaagtcctg gcgttgacct tgtcgcaact
     1561 tgatcttgag gcaggcacac ttcgccttga gccaggaaca acaaagaatg ccgaggggcg
     1621 gctagtctat ctcacgcccg aactgcaggc gctcatgagt gcccaagtcg atcgcgtgaa
     1681 agccttagcg cgacaactga atcgcctcac ccccttatct ctttccgcac gtgacaggca
     1741 ggtttcaagg gaaacgccgc cgggactttc gcaaggtctg gcacaccgcc ttggtacagg
     1801 tggggcttgt ggggatgttg cgccatgatt tccgccggac cgccgtccgc aatatggtca
     1861 atcttggtgt gcctgaacgg gtcgccatga agatcaccgg ccaccaaacg cgagcgtgtt
     1921 tgatcgctac catattgtga gtccagcgga tctccaaaac gctgcgcgaa cgctcacggg
     1981 cacaaacacg ggcacagtgg gcgaatcctc cttgactcct gcactgtaac tcgttaattt
     2041 ctttggcgtg gcggtgtagc tcagttggca gagcagcgga ctcataagcc gcgggtcacc
     2101 cgttcgatcc gggtcaccgc caccaaacca agcttgctcg taccggcggc tcatcaggcg
     2161 aagcctctct tcatgatcgc cgccggataa actccttcgg tgtttcttcc accccaatac
     2221 caaccttcgg ttgacccgct ggctaatttc cgttttttcg tgattgacgc cggcggataa
     2281 actcctttga cttt
//
LOCUS       DATMTI010000603         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_22744, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000603 DATMTI010000000
VERSION     DATMTI010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22744"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="VN039_09600"
     CDS             complement(<1..1677)
                     /locus_tag="VN039_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWV46256.1"
                     /translation="MKVLLIFPPDWYPSEPYLSLPSLTSVLRQAGHTVIQKDINCEMW
                     DWYFSEEFLKKVLRRVPQQLDRYRKLAKKRDLAEWEMDVQLALCDVSRPRMEKLIRDA
                     EEAKHIVRNEKFYDIDKLEWAIQVFREVTSVISLVYAPARICMPPMETDLSYKPYVSI
                     EVMDAVQDEQVNIYRDVFDHLVKPVIQQEQPDVVGISIVLQQQMFSSMTFCALIKQHF
                     PHIHITIGGNTVTRLRDVLPASPLFQYFDSAVVYEGETAFMQLIEAVGAKRSLANVPN
                     TIYKDETGVHVSPLSYAEDMAALPPPDFDGLPLEKYFVPTRVLPYLATRGCYWGRCEF
                     CDHGEGYTAGYRSKKIQDILADITYLRDKYGAKHFHFTDESYPPALFRKLSRGLIENK
                     MEIFWTTHMRFEKSLLEDAVWQDAKESGCRYLHFGYESGVERVLQLMDKATTAEVMTK
                     HLKFTAEAGIWNHCMGFFGFPGETKEEAWQSVQFLEQNKDHVHSLGFGTFDLGRHNPV
                     AKHPEKWGVTAYKNPDWDLALDYYYTVKNGMSIEEAERVFEQFERNHNPGW"
BASE COUNT          380 a          513 c          440 g          392 t
ORIGIN      
        1 ccagccggga ttgtggtttc gttcgaactg ctcgaacacc cgctccgcct cttcaatgct
       61 catgccgttc ttcactgtgt agtagtagtc cagtgccaga tcccagtccg gattcttgta
      121 tgccgtcacg ccccacttct ccggatgttt cgccaccgga ttatgccgcc ccagatcgaa
      181 tgtaccgaac ccgagcgaat ggacatggtc cttgttctgc tcgaggaact gcaccgactg
      241 ccaagcctct tccttcgtct cgccgggaaa gccgaagaag cccatacagt ggttccaaat
      301 gcccgcttcc gctgtaaact tcaggtgctt cgtcatgact tccgccgtcg tcgccttatc
      361 catcaactgc agcacccgct cgactcccga ctcgtagccg aagtgcagat agcggcagcc
      421 cgactccttc gcatcctgcc agacggcgtc ttccaacaga ctcttctcga accgcatatg
      481 cgtcgtccag aagatctcca ttttgttctc gatcaatcct cgagaaagtt tgcgaaacag
      541 cgccggggga taggactcat cggtgaagtg aaaatgtttg gcgccgtact tgtcgcgtag
      601 ataggtgatg tcggcaagaa tgtcctggat tttcttcgat cgatagcctg cggtatagcc
      661 ttcgccgtgg tcgcagaact cgcaacgtcc ccagtaacag ccccgcgtcg ccagataggg
      721 caagacgcga gtgggcacga aatacttctc aagcggcaat ccgtcgaagt ccggcggcgg
      781 cagcgcagcc atgtcctccg cataactcag gggagaaaca tggacgcccg tctcgtcttt
      841 atagatcgtg ttcggcacat tggccaaact ccgcttcgca ccgacggctt cgatcaattg
      901 cataaacgcc gtctctcctt cgtaaaccac ggcgctgtcg aaatactgaa agagcggcga
      961 tgcaggcagc acatcacgaa gacgagtcac cgtattgccg ccgatcgtga tgtggatatg
     1021 gggaaagtgc tgcttgatga gggcacagaa ggtcatggag gagaacatct gctgctgcag
     1081 cacgattgag atcccgacca cgtccggctg ttcctgttgg atcaccggct tgaccagatg
     1141 gtcgaacacg tcccgatata tgttgacctg ttcatcctgt acggcatcca tcacctcgat
     1201 agagacgtac ggcttgtacg ataggtccgt ttccatcggc ggcatgcaga tccgcgccgg
     1261 ggcatagacc agtgaaatga ccgatgtgac ctctcgaaaa acctgaatcg cccattctaa
     1321 cttgtcaata tcgtagaact tctcatttct gacgatgtgt ttggcctctt ccgcatcccg
     1381 gatcaacttc tccatccgtg gacgcgacac atcacagagc gccagctgca catccatttc
     1441 ccactctgcc aggtcccgct tctttgccag cttgcgatac cgatccaatt gctgcggcac
     1501 ccggcgtaaa accttcttta gaaactcctc gctgaaatac cagtcccaca tctcgcaatt
     1561 gatatctttt tgaatgactg tatgcccggc ttgacgaagg acagaagtga gagagggaag
     1621 gctcaaatag ggttcggagg ggtaccagtc cggcgggaag atcagcagca ctttcatctt
     1681 ccggccactg gcctcaaagg aggtccggcg gtggagctga atcag
//
LOCUS       DATMTI010000604         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_659217, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000604 DATMTI010000000
VERSION     DATMTI010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659217"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..800)
                     /locus_tag="VN039_09605"
     CDS             complement(51..800)
                     /locus_tag="VN039_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46257.1"
                     /translation="MRRRSIKGWTHHSGLGNLGLMARTAPRINRYSQLWTMVGGIVGS
                     TILFWIMVQLGQASSEPKYKTQPATTSELKRGVPPRVQDAEDSRAESDDDAKLFAPPV
                     TQGPAIDFPREVLEMLDQRKRDLDRREQTIRQNEERLMIVRSQVEELLDQNEALEKKI
                     QNAQAKVPAQQAKLQAEKERVAQDQRTQLAKMYEAMASEDAASRLERMPDRKAIEILK
                     MVKAKTAGAILAQVKAERAAKLTEQLLAQTP"
     gene            complement(797..1237)
                     /gene="fliJ"
                     /locus_tag="VN039_09610"
     CDS             complement(797..1237)
                     /gene="fliJ"
                     /locus_tag="VN039_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HWV46258.1"
                     /translation="MSLESLQKLRAQTVERLMMELAQVTRVLTQNEERCRAIEAQIQK
                     DVVVYDRQSAQGLTIEAWLEWQGRIDSQQAMLRRVRCEIDQAVEVWQRTKALLVEASQ
                     ERRLLDVVADKRREVQRAEAGRQEQRTTDEAASRRYSVGKASHS"
     gene            complement(1234..2541)
                     /locus_tag="VN039_09615"
     CDS             complement(1234..2541)
                     /locus_tag="VN039_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HWV46259.1"
                     /translation="MSLSRLIERIDPIEVSGRVAQAVGIVVEGYGPMTTVGELCRITR
                     EVVGEPIAAEVVGFRGDRVLLMPLGDMQGIGPGSRITMSGQVANLAVGPGLLGRVLDG
                     CGNPMDGKGPLTTTERYPLYAAAPNPLQRARLHTPLDLGVRAINGFLTCGRGQKMGIF
                     SGSGVGKSVLLGMISRYTKADVNVIALIGERGREVNEFLERDLGAEALGRSVVVVATS
                     DQAPLIRLRAALVATTIAEYFRDAGKQVLLLMDSLTRLAYSQREVGLAIGEPPTTKGY
                     TPSVFTLLPKLLERVGTGPGPGTITGLYTVLVDGDDLTDPIADAARSILDGHIVLSRT
                     LAARNHFPAIDLLQSTSRVMRDIVGRGHYEAARKLLELAARYRQSEDLVVLGAYKPGM
                     NTALDRAVQAQEAINGYLRQDIDQPAGLPSSVQQLEALAQQAA"
     gene            complement(2531..3556)
                     /locus_tag="VN039_09620"
     CDS             complement(2531..3556)
                     /locus_tag="VN039_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46260.1"
                     /translation="MTGTTIPALTVPHRSVPRLQGTVLVVDDDEGVRSLFLELLQREG
                     VPVRVSDSGQAALSMVKQTAPALLIVDVLLPDRDGIAVLEEAQRIDSRMIGIVMTGAA
                     SVELSIRAMQAGAVDFLMKPFHNDAVLATVRRALELHRLRNDYTVLKHAAVRSGGVRL
                     QSLPFQTFGDDGTLRGEDGLTDYERGLTEGRRHSEAQRRQDLAVLAEAVKKFDAARSA
                     LQRTVDDEVIALALQIASKILHESAESRREQIVVQVKAALGTVQESDGVVIQVHPADA
                     VILQEVREELAGRQDVALKLTIEPVASLQRGSCLLHTATRLVDASLDTQLFRLGDALR
                     NRACHES"
     gene            complement(3556..>4502)
                     /gene="fliG"
                     /locus_tag="VN039_09625"
     CDS             complement(3556..>4502)
                     /gene="fliG"
                     /locus_tag="VN039_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HWV46261.1"
                     /translation="AIGEEAAAQVMKQLDPKEIKRLGSFMNGTAHISRDEEDAVISDF
                     RTASASGEVQFQGKEFIKTVLIKALGPEKAARIIESMTRKSYPGLEALKWADAKTLVQ
                     MLKIEHAQTVAVILAHLESDQAGQVLAGLPESMRGDVALRLATMEEVQPDVLEELSQS
                     MQETLLASKGLGSQSIGGPEVIADILTRMDKANEGGIMVKIAEKSQPLADAIRALMFV
                     FDDLIKVDDRGVQELMKEISKDDLPLALRGANPEVKEKFFKNMSSRAAEMLKEDMEAK
                     GPVKVSDIEKAQQNILKVCRKLEEEGRIVIAGSGEEML"
BASE COUNT          844 a         1448 c         1194 g         1016 t
ORIGIN      
        1 gctcggcaat ctgtcgcagt ctatacccga ctgggaagat gatgtaaatt ctacggcgtt
       61 tgagccagca gttgttcggt gagtttcgca gcccgctcgg ctttgacctg cgcgaggatc
      121 gccccggcgg tcttggcttt caccattttg aggatttcga tcgctttccg gtccggcatg
      181 cgttcgagac gcgaggcggc gtcttcagac gccatggctt cgtacatttt ggcgagctgc
      241 gtccgttggt cctgcgccac acgctccttt tccgcctgga gcttcgcttg ttgagcaggg
      301 actttggcct gcgcgttctg gatcttcttt tccaaggctt cattctggtc caggagctcc
      361 tcaacttggc tcctcacaat catgagccgt tcttcgttct ggcgaatcgt ttgctcgcgc
      421 cggtcaagat cgcgctttcg ctggtccagc atctcgagca cttcacgcgg aaaatcgatg
      481 gccggccctt gtgtcacagg tggggcgaac aacttggcat cgtcgtcgga ttccgctcgt
      541 gagtcctctg catcctgcac tcgtggcggc accccccgct tcagttcact cgtcgtcgcc
      601 ggttgtgtct tgtatttcgg ttcggacgag gcttgtccca attgcaccat gatccaaaaa
      661 agaatcgtcg aaccgacgat cccccccacc atcgtccata gctgggagta ccggttgatc
      721 ctcggcgccg ttctcgccat cagcccaagg ttgccgagac cgctatgatg cgtccagccc
      781 ttgatggatc gtctcctcat gagtggctcg ctttgccgac ggaataccga cggctcgccg
      841 cttcatccgt cgtccgctgt tcctgacgcc ccgcctcggc tcgctgcact tcgcggcgtt
      901 tgtcggcgac aacatcaaga agcctgcgtt cctggctggc ttcgacgaga agcgccttgg
      961 tgcgctgcca cacctcaacg gcttggtcaa tctcgcaacg cactcgccgt agcattgcct
     1021 gttgcgaatc gatacgcccc tgccattcca gccacgcttc gatcgtcagc ccctgcgctg
     1081 attgtcgatc atagacaacg acatctttct gaatctgggc ttcgatggca cgacaccgtt
     1141 cctcgttctg agtcaagacg cgggtaacct gcgccaattc catcatcagg cgctccacag
     1201 tctgggcgcg cagcttctgc agcgattcaa ggctcatgct gcctgctggg ccagcgcctc
     1261 aagttgctgc accgacgacg gcaggccggc cggttggtca atgtcttgcc gtaaataacc
     1321 attgatcgcc tcctgtgctt gaacagcacg gtcgagggcg gtgttcatcc ccggcttata
     1381 cgctcccagc acaacgaggt cctccgattg gcgataccga gccgctaatt cgaggagctt
     1441 cctggcggcc tcgtagtgtc cccgtccgac gatatcccgc atcacgcggc tggtactctg
     1501 aaggagatcg atcgcgggaa agtgattgcg ggcagctaat gtgcgtgaca gaacgatatg
     1561 gccgtccaag atggaacgag cggcatcggc gatgggatcg gtcagatcat cgccatcgac
     1621 gagcaccgta tacagtccgg taatggtccc tggacccggt ccggtcccga cacgttccag
     1681 tagtttcggc aagagggtaa agacagaggg ggtatagcct ttggtcgtcg gaggctcccc
     1741 gatcgccaaa cccacttctc tctggctata ggccaatcgc gtcagggaat ccatcaacag
     1801 caggacctgt ttccccgcat cacggaagta ttcggcaatg gtcgtcgcta ccaacgccgc
     1861 acgcagtcgt atgagcgggg cttggtcgga ggtcgccacc acgaccaccg aacgcccaag
     1921 cgcctctgca cccaaatcgc gttccaggaa ttcgttgact tcacggcctc gttctccgat
     1981 aagggcgatg acgttgacgt ccgctttcgt gtaccggctg atcattccca acagcacgct
     2041 cttcccaacg ccggagccgg aaaaaatgcc catcttctgg ccacgtccac aggtaagaaa
     2101 tccgttgatc gcacgaacgc cgagatcaag cggagtatgg agacgggctc gttgcaacgg
     2161 gttcggcgca gccgcataca atggatagcg ctccgtggtg gtcaacggcc ctttgccgtc
     2221 catcggattt ccacagccgt cgagaaccct tcccagcagg cccggtccga cggcgagatt
     2281 ggccacttgg ccggacatgg taattcgact tcccggcccg atcccttgca tgtcccccag
     2341 cggcatgagg aggacacggt ctccgcggaa tcccacaacc tccgcggcaa tcggctctcc
     2401 gaccacctct ctcgtaattc gacaaagttc tccgacggtc gtcatcggcc catacccttc
     2461 cacgacgatc ccaactgctt gagccaccct tccggacact tcgatggggt cgatgcgctc
     2521 gatcagccga ctaagactca tgacaggccc tgttcctcag ggcatcaccc aagcggaata
     2581 actgggtatc gagggaggca tcgaccaatc gtgttgcagt atgcagaaga cagctgcctc
     2641 gttggagaga cgccaccggc tcgatggtca gcttcagtgc gacatcttgc cgtcctgcaa
     2701 gctcctcccg aacctcttga agtatgaccg cgtccgccgg atgcacttgg atcactacgc
     2761 catccgactc ttgaaccgta cctagtgccg ccttgacctg caccacgatt tgttcacggc
     2821 gggactcggc cgactcatgc agtattttgg acgcgatctg aagcgcaagc gcgatgactt
     2881 cgtcgtcgac cgtccgctga agcgcggacc gagcggcatc gaattttttg acggcctcgg
     2941 ccagcaccgc cagatcttgt cggcgctgcg cttcggaatg tcgccggccc tcagtcagcc
     3001 cacgctcata gtccgtcaga ccatcctccc ctcggagcgt gccgtcatcc ccgaaggtct
     3061 gaaacggcaa gctttgcagc cgtaccccgc cggaccgtac ggcggcatgc ttcaaaaccg
     3121 tatagtcgtt gcgcagccga tggagttcca gcgcacggcg cacggttgcc agcaccgcgt
     3181 cattgtgaaa aggtttcatc aagaaatcga cggcgccggc ctgcatcgca cggatggaaa
     3241 gttcgaccga ggccgctccg gtcatcacga ttccaatcat gcgactgtcg attcgctgcg
     3301 cttcttccag aactgcgatt ccatctcggt ccggtaataa gacatcgacg atcagcagag
     3361 cgggagctgt ctgcttcacc atggagagcg ccgcttgccc ggaatcggaa acccgaacgg
     3421 gcacgccttc ccgctgcaac agttcgagga acaaacttcg aaccccctcg tcgtcatcca
     3481 ccactaaaac cgtcccctga agtcgtggaa cgcttctatg aggaacggtc aacgccggga
     3541 tggtcgttcc cgtcactaca gcatctcctc tcctgaaccg gcgatgacga tccggccttc
     3601 ttcctcaagt tttcggcaga ccttcaggat attctgctgg gctttctcga tatcggacac
     3661 cttgacaggc cctttggcct ccatatcttc cttcaacatt tcagcggcgc gactcgacat
     3721 gtttttgaag aacttctcct tcacctccgg attggcgccg cggagagcca acggaaggtc
     3781 gtccttgctg atttccttca tcaattcctg tacgccgcga tcatcgactt ttatcaggtc
     3841 gtcgaagacg aacatgagcg ccctgatcgc gtccgccaac ggctggctct tctctgcaat
     3901 cttgaccata atgccgccct cgttggcctt atccatccgt gtgaggatgt cggcgatcac
     3961 ctcaggccct ccgatactct gtgaacccag tcctttgctc gccagcaacg tctcttgcat
     4021 gctttggctc agttcttcca agacatccgg ctggacttcc tccatggtgg caagccggag
     4081 cgccacgtct ccgcgcatcg actccggaag tcctgcaagc acttgcccgg cttgatcact
     4141 ttcaagatgc gcgagaatga ccgccaccgt ttgcgcatgc tcgatcttca gcatttggac
     4201 aagagttttc gcatctgccc atttcagcgc ctccagcccg ggatagcttt tccgggtcat
     4261 cgattcgatg atccgcgcag ccttttccgg acccaacgcc ttgatgagga cggtcttgat
     4321 gaactctttt ccctgaaact gaacctcgcc ggacgcgctg gcggttcgaa agtccgagat
     4381 gaccgcatct tcttcatctc gtgaaatgtg ggcggtgccg ttcatgaaac ttcccagccg
     4441 cttgatttct ttggggtcaa gttgcttcat gacctgggcg gccgcttctt ctccgatggc
     4501 ac
//
LOCUS       DATMTI010000605         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_786809, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000605 DATMTI010000000
VERSION     DATMTI010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786809"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VN039_09630"
     CDS             complement(<1..230)
                     /locus_tag="VN039_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46262.1"
                     /translation="MDRKYVASDPYPYPYNGDLRPDNTVFLIIDMQTDFCGKGGYVDK
                     MGYDISLTRAPIAHILKVLSKARTIGMHVFHT"
     gene            complement(274..1191)
                     /locus_tag="VN039_09635"
     CDS             complement(274..1191)
                     /locus_tag="VN039_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV46263.1"
                     /translation="MELGWWGVLIAVLGGAIRIGTPFLFVSLGECLTEKSGRINLGLE
                     GTLVMGAMTGYAVSLHSGSPWLGVLCAGLAGMMLGALHSWLCGFQRVNDIAVGIAMML
                     FGTGLAFFLGKPYIQPSAPQLPSIDLGGWSDIPQLQAALQINALFLVGIVVAIALGWM
                     LNNTAWGLKVRVVGESADAARALGLPVDRIRILSTAQGGFLAGIGGSYLSLYYPFNWS
                     EGLSSGQGLMAVALVIFARWQPLWCLWVSLLWGGAAALGPSLQSVGITGGYYIFNAIP
                     YIMTLAVMIITCSPRRTLVGVPHELTVTR"
     gene            complement(1191..2255)
                     /locus_tag="VN039_09640"
     CDS             complement(1191..2255)
                     /locus_tag="VN039_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV46264.1"
                     /translation="MSITVPQTLNKALEPFLIIVGSLTVSMVLFGIFVASAGADPFEV
                     YQTMYRAAFGTTFSWQNTLIRAAPLMLTALCTALPGYLGLIIIGGEGAIVMGGLFAAI
                     AALALPTAPPIAVLTTMTLVGMAAGGIWLTIGGALRHYRAVNETISSLLLNYIAIALL
                     NHLVTSTFRDPESLNHPSTHHIGEANMIGVIPGTDLHWGLVFGIVACVIAWFLMHHTV
                     FGFASQIAGGNVRAAQIAGLPVGRLILIICFVAGAAGGLAGMVEVAAIHGRANDTLNA
                     NYGYAGILVAFVARYNPLAIIPVAVLLGGIRASSGLLQRSHELPDATVLVLQGIIFLV
                     ILFSETLYGRFSKLRKQGAS"
     gene            complement(2252..>3103)
                     /locus_tag="VN039_09645"
     CDS             complement(2252..>3103)
                     /locus_tag="VN039_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWV46265.1"
                     /translation="KLDGAQVLFPTSFGYFDPHILKVAPQHPNILFLHCGGLYQDGKH
                     PKNVGSYFGYIDESQYVAGIVAGGMSKSGKLGFVAAKPIPQVLRNINSYTLGARSIRP
                     NCTTHVIFTGDWSMPVREAEATNSLVDQGIDVITCHVDSPKVVVTTAEKRGVYCTGYH
                     CNQSKLAPKGYLTGAEWNWAKVYMDYADMIRKGQTVPHLVRGGLKEGIVKVSDYNRVV
                     TEAVRKQADTAKDKFMSGGMVVYKGPIKDNAGRVVIAAGTEHVQTDIWLESMDWLVEG
                     VIGSTKS"
BASE COUNT          718 a          892 c          858 g          635 t
ORIGIN      
        1 ctggtgtgga agacatgcat tccgatggtt ctggctttcg acaggacttt gagaatatgc
       61 gcgatcgggg cacgggtgag agaaatgtca tagcccatct tgtcgacata accgcccttg
      121 ccgcaaaagt cggtttgcat atcgatgatg agaaagaccg tattgtcggg ccggagatcg
      181 ccgttatacg gataagggta ggggtcggat gcgacatatt ttctatccat agttcacctc
      241 ttaagcaggt ccattgggta tgtcattcgt tatctaacga gtcacagtca gctcatgcgg
      301 cactcctacc aacgtacgcc gaggcgagca ggtgatgatc atcaccgcca aggtcatgat
      361 gtaggggatt gcgttgaaga tgtaatatcc gcctgtgatt cccacggatt gcaaggacgg
      421 ccccaaggcc gccgcaccac cccataacag tgatacccag agacaccaca agggctgcca
      481 acgggcaaag atcaccagcg ccaccgccat caacccttgg ccgctcgaca gaccttcgct
      541 ccagttgaac gggtaataga gcgagagata ggacccgccg attccggcca agaatcctcc
      601 ttgagccgtg ctgaggatcc gtatgcgatc gaccggcaac cccagggcgc gcgccgcatc
      661 cgcgctttcc ccaacgaccc gcactttgag tccccaggcc gtattgttca gcatccaccc
      721 aagtgcgatt gccacgacga ttccaacgag aaacaaagcg ttgatctgca aggctgcctg
      781 gagttgtgga atatcactcc atccgccgag atcgatggac ggcagttgag gtgcggaggg
      841 ttggatgtat ggttttccta gaaagaaggc cagtcccgtg ccgaacagca tcatggcgat
      901 cccgaccgcg atatcattga ctcgttggaa gccgcagagc caactatgca gggcgccgag
      961 catcatgccg gccagcccgg cgcacaacac tcccaaccag ggagacccgc tgtgcagtga
     1021 gacggcatag cctgtcatcg cgcccatgac cagtgtgcct tccagtccca agttgatacg
     1081 accgctcttt tcggtcaggc attcacctag cgagacaaac aggaagggcg taccgattcg
     1141 aatcgctcct ccaagaacgg cgatgaggac tccccaccat ccgagttcca tcaggacgct
     1201 ccctgtttcc gcaactttga aaagcgaccg tacagggttt cgctgaagag gatgacgaga
     1261 aaaataatcc cctgcagcac gagcacggtg gcgtcgggca gctcatgaga tcgctgcagc
     1321 aatccgctgc tggcgcggat gcctcccaac aagacggcaa ccggaatgat ggccaacgga
     1381 ttatagcggg ccacaaaggc cacaagaatg ccggcatatc catagttggc attgagggtg
     1441 tcattggccc ggccatggat ggcggccacc tccaccatac cagccagtcc accggccgct
     1501 cctgccacga aacagatgat caagatcagc cgtccgaccg gcaagcccgc gatttgagcc
     1561 gcacgaacat tgcctccggc gatctgggat gcgaagccga acaccgtgtg atgcataaga
     1621 aaccatgcga tgacacaagc cacgatgccg aagaccaatc cccaatggag gtctgttcca
     1681 ggaatgacgc cgatcatatt ggcctctcca atatgatgcg tcgagggatg gttaaggctt
     1741 tccggatcgc gaaaggtgct ggtgacgagg tggttcaaca acgcaatggc gatatagttc
     1801 agcaacagac tgctgatggt ttcattgacg gcgcgatagt gccgcaatgc tcctccgatg
     1861 gtgagccaga ttccccccgc cgccatgccg acgagggtca tcgtagtcag aacggctatg
     1921 gggggagccg tcgggagggc tagagccgca atggctgcga agaggccgcc catcactatg
     1981 gccccttctc caccgatgat gatcaaaccc agatatcccg gcagggcggt gcagagcgcg
     2041 gttaacatca gcggcgcggc gcggatcaac gtattctgcc aggaaaacgt ggtgccgaag
     2101 gcggcacgat acatcgtctg atagacctcg aaaggatctg ctccagccga agccacaaag
     2161 attccaaaca ataccatcga gaccgtcaag gatccgacga tgataaggaa gggttccagc
     2221 gctttgttca gggtttgcgg tacggttatg ctcatgactt cgtcgaaccg atgacgccct
     2281 ctaccagcca atccatgctt tccagccaga tatcggtttg gacatgttcc gttcccgctg
     2341 caatgacgac acggccggca ttgtctttga tcgggccctt atacacgacc atccctccgc
     2401 tcatgaattt gtctttggct gtgtcggctt gtttgcgaac agcttccgtc accacccggt
     2461 tataatccga taccttgacg attccttcct tcagcccgcc gcgcacgaga tgcggaacgg
     2521 tctgcccttt tcgaatcatg tcggcataat ccatgtagac cttggcccaa ttccattctg
     2581 ctcccgtgag gtacccttta ggggcaagct tcgattggtt gcagtgataa ccggtgcaat
     2641 agacaccccg cttttctgcg gtcgtgacga cgaccttagg gctgtcgacg tggcaggtaa
     2701 tgacatcgat gccttggtcg accaagctgt tcgtggcttc cgcttcacgg accggcatgg
     2761 accagtcacc ggtaaaaatc acgtgggttg tacagttcgg tctgatgctg cgggcgccga
     2821 gggtgtagct gttgatattg cgcaagacct gtggaatagg ctttgccgcc acaaatccca
     2881 gtttcccgct cttgctcatt cctccggcga caatgcccgc cacatactgg gattcgtcga
     2941 tatagccgaa ataactgccc acgttcttgg ggtgcttgcc gtcctgatag agcccgccgc
     3001 aatgcaaaaa gaggatgttg ggatgctggg gcgcgacctt gagaatatgc ggatcgaaat
     3061 agccgaagga ggtgggaaac aacacttgcg ctccgtccag ttt
//
LOCUS       DATMTI010000606         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_532298, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000606 DATMTI010000000
VERSION     DATMTI010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532298"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VN039_09650"
     CDS             complement(<1..262)
                     /locus_tag="VN039_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46266.1"
                     /translation="MKERQAKSLRNPSSEDATQPILTHEKSLSQKIGQGSEAGDILDQ
                     QLVMLGFQLITQLNTLIKTSKIHGRANAALDKPVETMLTLIET"
     gene            complement(273..2069)
                     /locus_tag="VN039_09655"
     CDS             complement(273..2069)
                     /locus_tag="VN039_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV46267.1"
                     /translation="MTTDLKTAQEMMAAAVAARAAEDPEIVSVKRVLKLLDKTAKSNR
                     TYGSTNPVAQKFTQQLFEELTHHLAAHSKLAFLVQRSELLCKDSVVYQAEKDGGSESI
                     AFKLYADGIRELGLHQGLTQEDLSFFLASLWSDVDSNEDDDIVTRLWSRNLSTITLVT
                     AEELSKFSAGNDGFIHLDGSMSSSDSILRELLDRERERKKRGEAGADPERGGSGNAKS
                     RFQSGLAGYEVTEEELAVLAQEIEAERKQDSLIYILDMLTAILASEKSPALLTKLFSL
                     WGTVVESLLREGKWTVLENVLSLLHETDAVRPDLGEEHKQQLASLLNGLGRAERVKVI
                     ETYLNRNLDADVKGLSTILVLMKTDAVSSLCSLLANVTSPVHQAIVSEALAVLAKEHP
                     DFLLRGLSDRRPGYVRNLLSILIKWNNPKFADPVEKLIRYPDAQVRREVVRALGLFRP
                     NGNGMKLVSLTTDEDEGVRFAALKLLMSGQYIAPFSLWAPLLSEEEFMDRPISERRAV
                     FQAVRATCGDEAVPYWQGLMTEWVWMNRRKKEELAVIGAETLGKLATPAAVAALSLGA
                     QKGSAAVRQACVAALSQAQRLQCGVPSTGATP"
BASE COUNT          457 a          612 c          618 g          540 t
ORIGIN      
        1 gggtttcaat gagagtcaac atcgtttcga cgggtttgtc gagagcggcg ttggctcggc
       61 catggatttt cgacgtctta atcagagtat tcaactgtgt gatgagctga aatcccagca
      121 tcaccaactg ttggtcgagg atatctccgg cttctgatcc ttggcctatt ttctgcgata
      181 gcgacttttc gtgcgtcagg atcggttgtg tagcgtcttc gctggatggg tttcgcagag
      241 actttgcctg gcgttctttc acaatccgtt tcctagggag ttgcaccggt tgaggggacg
      301 ccgcattgca gccgttgagc ttgcgacaat gccgcgacgc atgcctgacg aaccgcagcg
      361 cttccctttt gggccccaag tgagagagcc gcgacggcgg cgggggtggc gagcttcccg
      421 agcgtttctg ctccgatgac cgccaactct tcttttttcc ttcgattcat ccagacccat
      481 tcagtcatga ggccttgcca atacggaact gcttcgtcac cgcacgtggc gcggacagcc
      541 tgaaagaccg ctcgacgttc actgatgggg cgatccatga actcttcttc cgacagaaga
      601 ggagcccaaa gggagaaggg agcgatgtac tggccggaca tgagcagctt gagtgcggcg
      661 aaacggaccc cttcgtcttc atcagtcgtg agggagacga gtttcatgcc attgccgttc
      721 ggccggaaca gaccgagagc acgaaccact tcccgacgca cctgcgcgtc tggataccgg
      781 atcaattttt cgaccggatc ggcgaacttg gggttattcc acttgatgag gatcgaaagc
      841 agattgcgaa catacccagg cctgcgatcc gataatcctc gcaacaagaa atccgggtgc
      901 tctttcgcca agaccgcgag ggcttccgac acaatggctt ggtggaccgg tgatgtcacg
      961 ttcgcaagaa gcgagcagag cgaggagacg gcatccgttt tcatcagcac caaaatggtg
     1021 gacaaccctt tcacgtcggc gtcgaggttt cggttgagat aggtctcgat caccttcacc
     1081 cgttccgcgc gaccgagtcc attgaggaga gaggccagct gttgcttgtg ttcctcaccc
     1141 agatcaggcc gcacggcatc cgtttcgtgg aggagactta agacgttctc caacacggtc
     1201 catttgcctt cgcgaagaag cgattccacg acggtgcccc acaggctgaa gagtttcgtc
     1261 agcaacgccg gagacttctc tgacgcgagg atcgcggtca gcatgtccag gatatagatg
     1321 agactatcct gttttcgctc tgcttcgatt tcttgcgcca ggacggcaag ctcttcttcc
     1381 gtgacctcat aaccggcaag tccggactgg aaacggcttt tggcgtttcc ggaaccgcct
     1441 ctctcggggt cggctcctgc ctcgcctcgt tttttgcgtt cccgctcgcg gtccaacagt
     1501 tctctgagta tcgaatccga cgaactcatg cttccgtcga gatggataaa gccgtcgttc
     1561 cctgccgaaa attttgagag ttcctcagcc gtcacgagcg tgattgtcga cagatttcga
     1621 gaccacaggc gcgtgacgat atcatcgtcc tcattcgagt cgacgtcgct ccataaggaa
     1681 gcgaggaaga acgacagatc ctcttgtgtg aggccttgat gtaagcctag ttctcggatg
     1741 ccgtcggcat acagcttgaa ggctatgctc tcgcttcctc catccttttc cgcctggtac
     1801 acgacagagt ccttgcacag caattcggat cgttggacaa ggaaagccaa tttagaatga
     1861 gccgcgaggt ggtgggtcaa ttcttcgaac agctgctgcg taaatttctg ggcgacggga
     1921 ttcgttgagc cgtacgtccg gttcgatttg gccgtcttat ccaatagttt taggacacgt
     1981 ttgacggaga caatctcggg gtcttcagca gcgcgagcgg cgactgcggc ggccatcatt
     2041 tcttgagctg ttttgaggtc cgtcgtcata cgtaagacct accggcagat caccgcttcg
     2101 tcaagaaaat cgcggttctt tacagtcgtt cgactctgga cacgtgcatg atccgtgact
     2161 tgtttgtcac gacaggcatc ggtagaatcg agccgcagta ctatgagcag tactcctctc
     2221 atcatct
//
LOCUS       DATMTI010000607         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_616431, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000607 DATMTI010000000
VERSION     DATMTI010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616431"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="VN039_09660"
     CDS             <1..1558
                     /locus_tag="VN039_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46268.1"
                     /translation="PLMVYVAYGISGLTGIVGTFFVKDYLGLSAAFLAALGFWAGIPW
                     ALKMPIGHTVDLVWRWKSWLVGLGAGLLTISLGIMALLIGDREAMTAIFPAEVWYVLS
                     VLLAPIGYVIQDVVADAMTVEAVPRVDDQGHPFDDQTCKLMHTTMQTLGRVAIVGGGI
                     FVALINVYVFSGTEGLPQAELIRLYKNIYLMAMVIPVVSVAGVGFAWWLKRRQMQRLI
                     QEGFSLEQVKKMVDVRDVENEPTKPNWWILGGSSAFVLFTLTVGFGGFPYREEIVFAG
                     SMSIVLFLMSRLVQELEPDKRITLVGTAAVIFALRAIPGPGPGSTWWMIDDLKFDQQF
                     LSVLSLIGGMLALMGMFAFRRFMAERSIMYVVGFLTIVGTILSLPIVGMYYGLHEWTV
                     KVTGGVVDARFIALVDTALESPLVQISMIPMLAWIANSAPANLKATFFAVMASFTNLA
                     LSFSQLGTKYLNEIFIVTREVRDQASGVLQTPADYSQLGDLLITQLVIGLALPFSAIL
                     FAKLTKFKSA"
     gene            complement(1632..>2194)
                     /locus_tag="VN039_09665"
     CDS             complement(1632..>2194)
                     /locus_tag="VN039_09665"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HWV46269.1"
                     /translation="EETALVELLKGKTTTMPQAHTGEGLFFTSRVGDTFSLKSHRIQI
                     EWNRAKEDVFVSQSRFTRGTLVQFSVRRGARQTLDNVFGEFAPQKYDFQFQKTEVRVK
                     LLQPDYVSRSEARRLLANLEKFREIIFDFRDVRSVGQGFADEVFRVFADRNPGIVLRP
                     ENAGPAVMAMIRHVSREASRNRQPQP"
BASE COUNT          442 a          576 c          619 g          557 t
ORIGIN      
        1 gccgctcatg gtctatgtcg cctatggtat ttcaggtctc accggcatcg tcgggacttt
       61 cttcgttaaa gactacctgg gactctccgc cgcatttctc gccgcgcttg gattttgggc
      121 cgggattccc tgggccttga agatgccgat cggccacacg gtcgatctcg tatggcgatg
      181 gaagagctgg ctcgttgggt tgggcgcagg gttgttgacg atcagcctcg ggatcatggc
      241 cctgttgatc ggtgaccgcg aagcgatgac ggcgatcttt cccgctgaag tttggtatgt
      301 cctcagcgtt ctgcttgccc cgatcggata tgtcattcaa gacgtcgttg cggacgctat
      361 gaccgtcgaa gcggttcccc gtgtcgacga tcaggggcat ccattcgatg atcaaacatg
      421 caagctgatg cataccacga tgcagacact cggacgtgtt gcgatagtcg gcggtgggat
      481 cttcgtggcg ctcattaacg tgtatgtctt cagtgggacg gaaggattgc ctcaagccga
      541 actcattcga ttgtacaaga acatctatct gatggctatg gtgatcccgg ttgtctcagt
      601 ggcaggagtc ggattcgcat ggtggctcaa gcggcggcag atgcagcgac tcattcagga
      661 aggattctca ctggagcagg tcaagaagat ggtggatgtg cgtgacgtgg agaacgagcc
      721 gacgaaaccg aattggtgga tcctcggcgg aagttcagct tttgtcctct ttacgttgac
      781 ggtcggattt ggcggatttc cctatcggga ggagatcgtg ttcgccggtt cgatgagcat
      841 cgtgctgttc ttgatgtcga gactcgttca ggaactggag cctgataaac ggatcacctt
      901 ggtgggcacc gcggcggtga tcttcgcctt gcgtgcgatt ccaggacctg gccctgggtc
      961 gacctggtgg atgatcgatg acctcaagtt cgatcaacag ttcctgtccg tcctctcctt
     1021 gatcggcggc atgttagcct tgatgggcat gttcgccttt cggcgcttta tggccgaacg
     1081 ttcgatcatg tatgtggtcg gatttttgac catcgtcggg acgatccttt ctctaccgat
     1141 tgtgggcatg tactacggat tgcatgaatg gacggtgaag gtgaccggcg gagtcgtcga
     1201 tgccaggttc atcgctctgg tcgatacggc actggaatcg ccgcttgtgc agatctccat
     1261 gattccgatg ttggcatgga tcgcgaattc cgctccggcc aatctgaaag ccaccttctt
     1321 tgccgtgatg gcctccttta ccaatctcgc cctttctttc agccagctcg gtaccaagta
     1381 tctcaacgag atcttcatcg tcaccaggga ggtacgggat caagcgagcg gtgttctcca
     1441 gactccagcc gattacagcc aactcggcga tctcttgatc acgcaattgg tgatcggcct
     1501 ggctcttccc ttttccgcca tcttgtttgc gaagctcacc aagttcaaaa gcgcgtgacc
     1561 ttaccttcat tagtgcggtt atcgagattg actccgccgg cgtttctttc ccttagacgg
     1621 tccataactg attacggttg cggctgccgg ttgcgggaag cttctcggct cacatgcctg
     1681 atcatggcca tgacggcggg accggcattt tcggggcgaa gcacaatacc ggggttgcga
     1741 tcggcaaaca cgcgaaacac ttcgtctgcg aacccttggc ccaccgagcg cacgtctcga
     1801 aaatcaaaga tgatctcacg gaacttctcc aagttagcca aaaggcgtct tgcctcagag
     1861 cgtgaaacat agtcaggctg caaaagcttg acccgtactt cagtcttttg aaactggaaa
     1921 tcatactttt gaggagcgaa ttcaccgaac acattgtcga gagtctggcg ggcaccacgc
     1981 cgaaccgaaa actgtacgag ggttccacgc gtgaaacgcg actgtgaaac aaacacgtcc
     2041 tccttggccc gattccattc aatttgtatc cggtgggact tcaaggaaaa tgtatcaccg
     2101 acccgtgatg tgaaaaaaag tccttcgccg gtgtgagctt gaggcatggt ggtcgtctta
     2161 cctttgagaa gctcaacgag cgctgtctct tcat
//
LOCUS       DATMTI010000608         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_786891, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000608 DATMTI010000000
VERSION     DATMTI010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786891"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..483)
                     /locus_tag="VN039_09670"
     CDS             complement(214..483)
                     /locus_tag="VN039_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46270.1"
                     /translation="MKGLRFERIAKGRHYNVVFHIGSTYVPVSDDTVEELKGQSLLPA
                     ERFFDFLIDRVGYSSYLKDQIRNELKATGDPTTQITVLQGAIREL"
     gene            631..2085
                     /locus_tag="VN039_09675"
     CDS             631..2085
                     /locus_tag="VN039_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HWV46271.1"
                     /translation="MSATELIELWITRLEAERVRLTETGQDAPVVASQGRLVQTTGGL
                     HLYEFIVPDDVQLSVDLPVSVILADEADTTEGIVLRQIGNRVYVQLVDPLGRDVPSVT
                     LVPDQAGLVGTSASRLKDMLAKPDLYHLGPAERLASLLQMQEAEAAAFSSASSVFTTM
                     WSDDRSFRRQKLANLAMELIRANKRILLISPDHQECDEIAGMVGRTMKGGGLNHKTWI
                     TRYELPIASQAGGVDLHELGFEAQMHQFYAKSQGDKASLKHKYDRFRELAPFLSQKEA
                     KQKDLDEVRLLEWRLVTQLRDLQVRMADVRKTLGDFENLPLFQRLTMQTVGKNAESLK
                     QYCALYQGQMDQLNKELDVAKGRIQQLAPEAAIPRGTRAEFNELKEYIAKLGGTKKVR
                     ELMAAEENPNRQAFIQNRRVVAATPTRVASDPLFSRVRFDVLMVDEAPRIAAASLLAA
                     AGLVRERIIVSGDPRDIAAAGQWAMSQAAIRAAQ"
     gene            2298..2384
                     /locus_tag="VN039_09680"
     tRNA            2298..2384
                     /locus_tag="VN039_09680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2332..2334,aa:Leu,seq:cag)
BASE COUNT          589 a          669 c          697 g          526 t
ORIGIN      
        1 agaactaagg tggtcggtcg gccatgaccg tcgctgatac gaatttgtga tttctctatg
       61 gccaaaggat ggaaaactac caccaagttg ccagagttac aggcaaaaac cttggcaaca
      121 tggccaaaga tcgaaattca agatttcaga atgtgaggca taggtcgagg cagaaagagg
      181 acgagaagag agcgagcgga aatttagcag gcattacagc tcgcgaatcg caccctggag
      241 gaccgtaatc tgtgtggtgg gatcgccggt cgctttcagt tcgttgcgga tctgatcctt
      301 caggtaggac gagtagccca cgcgatcaat gaggaaatcg aaaaatcgtt cggccggtaa
      361 caggctttgt cccttgagct cttctaccgt gtcgtcactc accggcacat acgtgctgcc
      421 gatgtggaag accacgttgt agtgtcgtcc tttggcaatc cgctcaaacc gtaagccctt
      481 cacaggttcc tccacaagaa ggggctcatt ctagacaaga tccggtcatg aagacaaggg
      541 caacctctcg atccctgcgg tggacaggta tcgcggcagg catttatcat gaccggtcct
      601 tgccttgaga tgcagatgag atcagtcctc atgagcgcaa cagaactcat cgaattgtgg
      661 atcacccgcc ttgaagccga gcgagtccga ctgactgaaa cagggcagga tgctcctgtt
      721 gtagcgtcgc aaggtcgcct ggtgcaaaca accggtggac tccatctgta cgaatttatc
      781 gtgcccgacg acgtccaact gtccgtcgac cttccggtat ccgtcatcct tgccgatgag
      841 gcggacacga cggaaggaat tgtcctccgg cagatcggga acagggtata cgtccaactg
      901 gtcgatcctc tcggacgcga cgtgccgtcc gttacccttg ttccagatca ggccggtctt
      961 gtcggcacgt cagcctcccg tctgaaagat atgctggcga agccggatct gtatcacctc
     1021 gggccggccg agcggttggc ttcgctgttg cagatgcagg aagctgaggc tgcagcattc
     1081 tcctccgcct catccgtctt cacaacaatg tggtcggatg atcggtcatt ccgtcgtcag
     1141 aagctcgcaa atctggcgat ggaactcatt cgcgccaaca aacgtatcct cctgatcagt
     1201 cccgatcacc aagagtgcga tgagattgcg gggatggtgg ggcgtaccat gaagggcggc
     1261 ggactgaacc acaaaacatg gattactcga tatgaactcc cgattgcttc acaagccggc
     1321 ggcgtcgatc ttcacgaact ggggttcgaa gcgcaaatgc atcaattcta tgccaagtca
     1381 caaggcgaca aggcctcctt gaaacataag tacgaccgct tccgagaact cgcacctttt
     1441 ttgtctcaaa aagaagcgaa gcagaaggat ctcgacgaag tccgcctgtt ggaatggcgc
     1501 ctcgtcacgc aattgcgaga tcttcaagtg aggatggccg atgtccggaa gacactcggg
     1561 gacttcgaga acctaccttt gtttcaacgt ctgacgatgc agacggtggg gaaaaatgcg
     1621 gagtccctca aacagtactg cgcgctgtat cagggtcaga tggaccagct gaacaaagaa
     1681 ctcgatgtcg ccaaaggccg cattcagcag cttgctccgg aagcggccat tcctagaggg
     1741 acgcgagccg agttcaacga gctcaaggag tacatcgcga aactcggtgg cacgaaaaag
     1801 gttcgtgagc tgatggctgc tgaggaaaat ccgaaccgcc aggccttcat tcagaatcgg
     1861 cgtgtcgttg ctgccacacc cacgcgagtc gcgagcgatc ccttgttcag ccgggtgcgc
     1921 ttcgatgtct taatggtcga tgaggcccca aggatcgcgg cggcttcact tctggccgcg
     1981 gcaggactcg tacgtgaacg tattatcgtc agcggcgatc cacgtgacat cgctgccgca
     2041 ggacaatggg ccatgtctca ggcagccatt cgtgccgcac agtgagcagc agcctatgac
     2101 acttgcttga cgtgggtaac gagaattcag gataatccaa actcctttcc ggtcggggaa
     2161 atctttgagg ttgctcaaaa tgttttccgg caaggccgca aggagcgcag gccccgaggc
     2221 gtactttttt gttcgtacgt tgaggggaat gggcgactga gaacgccgcc ggaagacatt
     2281 ttcagcaacc gagtattgcc gaagtggcgg aactggcaga cgcactagat tcagggtcta
     2341 gcgccctcac gggtgtccgg gttcaaatcc cggcttcggc accattcctg taaatccact
     2401 tagctagcct caaatgacga cttgagtgta ccgcgctaca cttcggaagc cgctgcgtcg
     2461 tactcttccc gacatataga g
//
LOCUS       DATMTI010000609         1621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_64559, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000609 DATMTI010000000
VERSION     DATMTI010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64559"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VN039_09685"
     CDS             complement(<1..430)
                     /locus_tag="VN039_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46272.1"
                     /translation="MSTITTHRLAGILMILACGLSVACVPKIDPLALNIPAKEQEVPA
                     VLVVGPIDVTKLSYKGQPLSVDMEQAVQSTIVDSLKRTQVFKDVLLLEKPSQVGFSKP
                     DTEKILAAARAQNADLLLVGEVKQFETDVPVFMFGSHYDIK"
     gene            complement(453..812)
                     /locus_tag="VN039_09690"
     CDS             complement(453..812)
                     /locus_tag="VN039_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46273.1"
                     /translation="MRHHVSRLKGGLFQCVGSLILLSSMACTITPGAIAPTSLPIPGR
                     YVELSKLEEASSCGYTLGTVPLKNPKPVSEVIDELIKSRGGEALVDVTSNSTVMFYGI
                     GMENCVNVKAKVINIAR"
     gene            complement(821..1183)
                     /locus_tag="VN039_09695"
     CDS             complement(821..1183)
                     /locus_tag="VN039_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46274.1"
                     /translation="MTSIRPRQLLVLLPAVLCLLSLNACMVSRHPAGIAASNGPLPQS
                     YTTLDAGKKSSCGYQFMFIPITGKDDTDQIISELINEKGADALVGVTVERELSLFALP
                     VVGSDCTVVKGLAVKKSQ"
     gene            complement(1202..>1621)
                     /locus_tag="VN039_09700"
     CDS             complement(1202..>1621)
                     /locus_tag="VN039_09700"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HWV46275.1"
                     /translation="AYQNGDDAEIRVTPSEDAYLYIFGVSDDDHITILAPNRYYPNTL
                     VKGGTDFIFPPDTLVKRGIRLQTWAVPGKPKTVEKIKVIASKRTMDALKGKIQEAMFA
                     SYSPSDHTMLVHLLRSLATFDPSEWAENTVTYEVYAK"
BASE COUNT          370 a          424 c          420 g          407 t
ORIGIN      
        1 tttttatgtc atagtgggat ccgaacatga ataccggaac atccgtttcg aattgtttaa
       61 cttcgccgac aaggagcaga tcggcgttct gtgccctggc agcggccaga atcttttcag
      121 tatccggctt gctgaacccg acttgcgacg gcttctccag cagaagcacg tccttgaaga
      181 cttgtgtgcg tttgagagaa tccacgatgg tggattgcac ggcctgttcc atatcgacgc
      241 tcaacggctg acctttatac gacagcttcg tcacgtctat cggtccaaca accaatacgg
      301 ccggcacttc ttgctcctta gcgggaatat tcaacgcgag cggatcgatc tttgggacac
      361 atgccacaga cagtccacag gcgaggatca tcaaaatacc ggctagtcgg tgagttgtga
      421 tggtcgacat gttcgctctc cacgataatc gtttaccggg caatattgat cactttggcc
      481 ttgacgttca cgcagttctc catacctatg ccgtaaaaca tcactgtaga attggaggtg
      541 acatcgacta atgcctcgcc accccggctc ttgatgagtt cgtcaatgac ctccgatacg
      601 ggcttggggt tctttaacgg cacagtgccc agcgtgtacc cgcatgaaga tgcttcttct
      661 aatttgctga gctcgacata tcgtcccgga atggggaggc tggttggggc aattgcccct
      721 ggagtgatgg tgcatgccat cgacgagagg aggataaggc ttccgacaca ctgaaatagg
      781 ccgcccttta agcgcgagac atggtgtcgc atgatggatc tcattgtgat ttcttcacag
      841 ccaaaccttt gacgaccgtg caatccgatc cgactacagg aagagcaaac agcgatagct
      901 cccgttccac cgtgaccccg accaaggcat ctgcgccctt ttcgttgatg agttcggata
      961 tgatttggtc cgtgtcgtct tttccggtga tcgggatgaa catgaattga tagccacatg
     1021 aagacttttt gccggcatcc aaggtcgtat agctctgagg taatggtcca ttggatgcgg
     1081 cgatacctgc cggatggcgg ctgaccatgc aggcgttgag cgagagcagg cacagcaccg
     1141 ccggaagaag cacgagcaac tgacgtggtc ttatactggt catggtcatc tcgccgacga
     1201 attatttggc atagacttca tacgtcaccg tattttcggc ccattctgag ggatcgaacg
     1261 tcgcaagact ccgcaagaga tggaccagca tcgtatgatc cgacgggcta tagctggcaa
     1321 acatcgcttc ctggatcttg cctttcagcg catccatggt tcgcttgctt gcaatcacct
     1381 tgattttttc tacagtcttc ggcttgcccg gcacggccca tgtttgaagc cggataccac
     1441 gcttgaccag tgtgtcggga ggaaatataa aatcggtgcc tcctttcacc aaggtatttg
     1501 ggtaataccg gttcggcgcc aagatcgtaa tatgatcatc gtcggacacc ccgaaaatat
     1561 agagatacgc atcctcggat ggagtgacac gtatttccgc gtcatcaccg ttttgatagg
     1621 c
//
LOCUS       DATMTI010000610         1634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_616647, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000610 DATMTI010000000
VERSION     DATMTI010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616647"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VN039_09705"
     CDS             complement(<1..326)
                     /locus_tag="VN039_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HWV46276.1"
                     /translation="MDISKLLTFSVKEGASDCHISAGEPPMIRIHGDLKKLDHPPLTP
                     DETHALIYDMMNDAQRKTFEEKRECDFSFELGDIARFRVNVFVQQRGLGAVFRNIPTT
                     ILPLEKL"
     gene            complement(445..>1634)
                     /gene="bioF"
                     /locus_tag="VN039_09710"
     CDS             complement(445..>1634)
                     /gene="bioF"
                     /locus_tag="VN039_09710"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWV46277.1"
                     /translation="EALKKVSNHMFRSKLKELADRSLMRRLSPLESGTGPTIKYAGRD
                     VILLSSNDYLGLATHPEVIRAAVHATEQYGTGSGASRLVSGTLPPHIHLETSLASFKG
                     TEAALLFGAGYLANIGVIPNLIEQGGLILADRLCHASLIDGCRLSRADFRVFCHRDCG
                     HLESLLRRRRANRPTLIITEGLFSMDGDLAPLSDLESLAEQYEAMLYVDDAHGTGIMG
                     PTGRGTIEHFGLEQRIPFHMGTMSKALGSHGAYIVGPNDLIQYLINVTCPFIFTTALP
                     PAIAAAAAAAVAVIRGEPERRMKLWSNRQQLFNGMQNLGFRMTQTVSPILPMLVGDAA
                     VASAFAERLLTHGIYASAIRPPTVPDGTSRIRFTVTSEHTTEQIDEALRALDLSGRET
                     GLL"
BASE COUNT          369 a          429 c          478 g          358 t
ORIGIN      
        1 agtttttcca acggaagaat ggtggtcgga atattccgaa agacggcgcc taacccccgc
       61 tgttgcacga acacgttgac gcggaaacgg gcgatgtctc cgagttcgaa cgagaagtca
      121 cattcacgct tctcttcgaa agtctttcgt tgggcgtcat tcatcatgtc gtaaatgagg
      181 gcatgtgttt cgtcgggagt gagaggagga tggtcgagtt ttttcagatc gccgtgaata
      241 cgaatcatgg gaggttcacc ggcactgata tggcagtcgg aggcaccttc ctttaccgag
      301 aaagtcagca gtttggaaat atccatcgcc cgtgatcctc agtgttatgg gtcgctcgtg
      361 aggtggaaat ctccagtgca tcatgccata gcaaagcccg aaagcaagaa aatagccgga
      421 aggcgcgtgc caataaggaa gcgattagag gaggccggtc tcacgacccg agaggtcaag
      481 ggcacgaagc gcttcgtcga tctgctcagt cgtgtgttcc gatgtcacgg tgaagcgtat
      541 gcggctggtt ccgtccggaa cggtcggcgg ccgaattgcg gaagcataga tgccgtgagt
      601 gagcagtcgt tcggcgaatg cagatgcaac ggctgcatcg cccaccagca tcggcagaat
      661 cggactgacg gtctgagtca tccgaaaacc aagattctgc attccattga acagctgttg
      721 tcgattcgac cagagcttca tccgacgttc cggttcaccc cgaatgaccg cgaccgcagc
      781 cgcggctgcc gccgcgatcg cgggtggaag ggcggttgtg aagatgaacg gacaggtcac
      841 gttgatcaaa tactgaatca agtcgttggg cccaacgatg taggcgccat ggctccccag
      901 tgctttactc atcgtcccca tgtgaaaggg gatccgctgc tccagaccga agtgttcgat
      961 cgttccgcga ccggtcggtc ccatgatgcc ggtcccgtgg gcatcatcga catacaacat
     1021 tgcctcatat tgctcggcca gcgattccag atccgacaat ggagcgaggt cgccatccat
     1081 gctgaatagc ccttccgtga tgatgagcgt ggggcgattc gctctccgtc gtcgcagtaa
     1141 tgactcaaga tgtccacagt cccgatggca gaatacccga aaatcggccc ggctcaatcg
     1201 acatccatcg atgagactgg catgacacaa tcgatccgct aggatcaggc ctccttgctc
     1261 gatgaggttc ggaatgacgc cgatatttgc taaataaccg gctccgaaca gaagcgcggc
     1321 ctctgtccct ttaaacgacg cgagggaggt ctcgaggtgt atatggggag ggagtgttcc
     1381 actgaccagg cgagaagctc ctgacccggt cccatactgc tctgttgcat gtacggcagc
     1441 gcggatgact tccggatggg tcgcgagtcc caggtaatcg ttggaggaca gcaaaatgac
     1501 gtcccgtccc gcatatttga tggtcggccc ggtgcccgat tccaatggag atagtcggcg
     1561 catgagcgat cggtcagcga gttctttgag cttgcttcga aacatgtgat ttgaaacttt
     1621 ctttagagct tcgt
//
LOCUS       DATMTI010000611         4505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_236482, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000611 DATMTI010000000
VERSION     DATMTI010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236482"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VN039_09715"
     CDS             <1..417
                     /locus_tag="VN039_09715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWV46278.1"
                     /translation="NKRWRSRRIGAGIGDPGPCGKILIRGEPGWEIIPELAPKPGEPV
                     IDKPGKGSFWATDLDMLLRLRGINNVILGGITTDVCVHTTMREANDRGFECLLLEDCC
                     GATDLGNHQAAIKMVTMQGGVFGAVAKSHALLEALS"
     gene            414..1931
                     /locus_tag="VN039_09720"
     CDS             414..1931
                     /locus_tag="VN039_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV46279.1"
                     /translation="MTGGVHPDLQALGITKRFGPLTALKDVNFRLRSSRFHALLGENG
                     AGKSTLVKCIMGYYHPDEGTILLDQQPVTIRNPRDAQTFGIGMVYQQFTLIPNMTVLE
                     NLVISKAKLNPIINWQAEESQLTEFMEKMPFKVPLSARVGSLAAGAKQKVEILKQLAL
                     QNRILILDEPTSVLTPQEADEVLGHLRAMTLAQQLSVLIITHKLREVAAFADEVTVLR
                     KGQFVGSGLVSELKISQMAEMMVGSEVTAKPKQRTVLSKKVPKLVLQDIRVKDDLGVE
                     VVRGLHLTIHEGEIVGVAGVSGNGQRELVEVLAGQRQAVAGTIRVQDQPFQPTREKIM
                     QHKMFCLPEEPLQNTCVGRMTVSENLAFRNFDVVPHAIGGWLISRSALRRSAFGLIER
                     YNIKPPYPDIPIQSLSGGNVQRAVLARELSAEVEILIIANPCFGLDFLAMEEIRLQIV
                     AARNRGTAVLLISEDLDEILELSDRTVVMFNGQLVYETTSSDLAIIGRHMTGHTT"
     gene            1928..2635
                     /locus_tag="VN039_09725"
     CDS             1928..2635
                     /locus_tag="VN039_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HWV46280.1"
                     /translation="MNTVIASPYPYPLPTKDSGNQVALVIIDMQRDFVEPGGFGAALG
                     NTVEHLQKIVPTVARLLKTFRELRLPVIHTREGHRPDLSDCPPAKIRRGDSTLRIGDP
                     GPMGRILVSGEPGNDIIPELHPEAGELVIDKPGKGAFYATNLQENLKTLGVTHLIFTG
                     VTTEVCVQTSMREANDRGFECLLVEDGTESYFPEFKQATVRMIQSQGGIVGWVTTADR
                     LIASLRQTYAGNAHEPE"
     gene            2622..3023
                     /locus_tag="VN039_09730"
     CDS             2622..3023
                     /locus_tag="VN039_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46281.1"
                     /translation="MNQSDVLLAVNGTLMRGLELNPNMVAAKATFVRETMTEATYRLW
                     TINDEHPAMLRVTDGTGVKVAVEVWSVPAEGLAGILLNEPLGLCIGKVRLEDGTVVLG
                     VLGEPALVEKHREITGYGGWRSYLAQRLTAS"
     gene            complement(3190..>4505)
                     /locus_tag="VN039_09735"
     CDS             complement(3190..>4505)
                     /locus_tag="VN039_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV46282.1"
                     /translation="DALLLDQSRVDRRTIIGGIYERQIDANTVLTVEADYDVKDIQQS
                     FSQIFENTNPNYKSYADLRHDGRLGVMPLKSYVGFFVNKMEQKGNTFQNLADGFGTKG
                     NLIQNSRGTIFNIGGRIREELEFYPNWILAVGLGFEQSRLSVDATNYDQNGTLASRVS
                     ANRTFSNWAPEGSLTWKPSADYRYWLRASTGYGIPQFSNLLRDPVTGLPGSNFSLKPQ
                     KNLNLEIGTEMRLHPTLLVHVVGFYTFFKDEIITQVISGNNTASVNADSSRYRGVELF
                     ADWRPVTGLRLSGAYTHINSEYINFSDRTAAGFIVRDGKHVPNVPTDILNGKVEYYHA
                     PMGLGGWVEGNYSNSYFLNNNNTFGFPSYVIGNVNVYKNIEVSTSSWFRFAKFFIQVD
                     NIADTKYAASGQVITGETHAAAAGQQIFFAGYGRAVYGGVTLGLF"
BASE COUNT         1012 a         1240 c         1287 g          966 t
ORIGIN      
        1 aataagcggt ggcgcagccg gaggatcgga gccggcatcg gagatcccgg cccttgcggg
       61 aaaatcctga ttcgaggaga gccgggatgg gagatcattc ctgaactcgc tcctaagccg
      121 ggcgaaccgg tcatcgataa accagggaaa ggctcgttct gggcgaccga tttggacatg
      181 ctgttgcggc ttcgcggcat caacaatgta attcttggcg ggatcaccac cgatgtctgt
      241 gtccatacca ccatgcgcga agccaatgat cgaggttttg aatgtctctt actggaggat
      301 tgttgcggcg caactgacct tggaaatcac caagctgcga tcaagatggt cacaatgcag
      361 ggcggagtgt tcggcgccgt ggcaaagtct catgccctgt tggaggcgct ttcatgacgg
      421 gtggagttca tccggatctg caagcgttgg gaattaccaa acgcttcgga ccgctcacgg
      481 ccttgaaaga tgtgaatttt cgactgcggt cgagtcggtt tcatgcactg ctcggtgaaa
      541 acggcgccgg gaagagcacc ctggtcaaat gcatcatggg gtattaccac cctgatgagg
      601 ggactattct gttggatcag caaccggtca cgattcgcaa cccgcgcgac gcgcagacct
      661 tcggcatcgg catggtgtat cagcagttca ccctcatccc caacatgacg gtcctcgaga
      721 acctggtcat ttcaaaggcc aagttgaatc ccatcattaa ttggcaggca gaagaatctc
      781 aactgacgga gttcatggag aagatgccgt tcaaggtacc gctctccgct cgcgtcggtt
      841 cgctcgctgc aggcgcaaaa caaaaggtcg aaatcctcaa acaactcgcg ttacagaacc
      901 gaatccttat tctcgacgaa ccgacctccg tcctgacgcc acaagaagcg gatgaagtct
      961 tgggccatct ccgggctatg accctggccc agcaattgag cgtcctgatc attacccaca
     1021 agcttcgaga agtggccgca tttgcggacg aggtcacggt cctgcgcaag ggacaattcg
     1081 ttggctccgg actagtttcg gagctgaaaa tttcacagat ggctgagatg atggtgggct
     1141 cggaagtgac ggcgaagccg aagcagcgga ccgttctgtc aaagaaagtt ccgaagctgg
     1201 tcctgcaaga tatccgcgtc aaggatgatc tcggcgtgga ggtcgtgcgg gggctgcatc
     1261 tcacgatcca tgaaggcgag atcgtcggag tggccggggt atcgggcaac ggccagcgag
     1321 aactagtcga agtcctggcg gggcagcgcc aagccgttgc cggcacgatc cgcgtgcagg
     1381 atcagccttt tcagccgact cgcgagaaaa tcatgcagca caagatgttt tgcctgcccg
     1441 aagaaccgct tcagaacacg tgcgtgggac gcatgacggt ctcggagaac ctggcgtttc
     1501 gtaatttcga cgtcgtacct cacgcgatcg gcggttggct catcagtcgt tctgcactgc
     1561 gccgcagcgc gttcgggctg attgaacgct ataacatcaa accgccctat cccgacattc
     1621 ccattcaatc gctctcggga ggcaacgtgc aacgcgcggt cttagctcgc gagctgtcgg
     1681 cggaggtcga gattctgatc atcgccaacc cctgtttcgg gctggacttt ttggccatgg
     1741 aggaaattcg gctgcaaatt gtcgccgcgc gcaatcgcgg gacggcagtg cttctgatca
     1801 gcgaagatct cgacgagatc ctggaactgt ccgaccgcac ggtggtcatg ttcaacggtc
     1861 aactcgtcta tgaaaccacg tcgtccgacc tggcgatcat tgggcgacac atgacaggac
     1921 atacgacatg aataccgtca tcgcctctcc gtatccttat ccattaccga cgaaagactc
     1981 aggcaatcaa gtggcgttgg tgatcatcga tatgcagcgc gacttcgtcg agccgggcgg
     2041 attcggcgcg gcgcttggaa atacggtcga gcatctgcag aaaatcgtgc cgaccgtcgc
     2101 gaggctcctc aagacattcc gcgaattacg gttgccggtg attcacacga gggagggcca
     2161 tcgtcccgat ctctcggatt gcccgccggc caagatacgg cgcggagatt ccacgctccg
     2221 tatcggcgat cccggtccga tgggacgcat tctggtctcg ggagagcctg gaaatgatat
     2281 catccctgag ctgcaccccg aggccggcga actcgtcatc gacaagccgg gcaagggggc
     2341 attctacgcc accaatttac aggaaaacct aaagacactc ggtgtcaccc atctgatatt
     2401 tacaggagtc accaccgaag tctgtgtcca aaccagcatg agagaagcga acgaccgggg
     2461 ctttgaatgt ctcctggtcg aggatggaac cgaaagttat tttcccgagt ttaaacaggc
     2521 gacggtccgc atgatccaat cgcagggagg catcgtcggc tgggtcacga cggccgatcg
     2581 tctcatcgca tcgcttcggc aaacctacgc agggaacgcc catgaaccag agtgacgtgc
     2641 tgctcgcggt caacggcacg ctgatgcggg gactcgagct caaccccaac atggtggcgg
     2701 ccaaggcgac gttcgttcgt gagacgatga ccgaggccac gtatcgtctc tggacgatca
     2761 acgatgaaca tcctgcgatg ctgcgtgtca ccgatggaac gggcgtcaag gtggcggtcg
     2821 aagtctggtc agtgcccgcc gaagggttgg cgggaatcct gttgaatgag ccccttggtc
     2881 tgtgtatcgg aaaggtccgt ctggaagacg gaaccgtcgt cctcggtgtg ctgggtgagc
     2941 cggcgctcgt tgaaaagcac cgagagatca cggggtacgg aggctggcgc agttatttgg
     3001 cgcagcgtct taccgcgtcc tgacctgatc catgtcgtgt tcatataaca gcggggcgga
     3061 ggccaaggga ggcacacctc caccccgcgc tggtccgaga gaggaggcaa ccaacctcgg
     3121 accgatcatg cttttacgat tcaatcgcct gtgagcggga ggcatgtagt ttctcgctca
     3181 cggcatgcgt taaaacaggc ccagcgtcac acctccatag accgctcgtc cgtagccggc
     3241 gaagaagatc tgttgacctg cggcagcggc atgcgtttcg ccggtgatta cttgcccgga
     3301 ggcggcatat ttcgtatctg cgatgttgtc cacttggata aagaacttgg caaaccggaa
     3361 ccatgaggat gttgagacct cgatattctt gtacacattg acgttcccaa tcacgtaaga
     3421 gggaaatccg aacgtattgt tgttgttcaa gaaatagcta ttggagtaat tcccttcgac
     3481 ccaacccccc aatcccatcg gggcatggta gtactcgacc ttgccgttca agatatcggt
     3541 cggcacattg ggcacgtgtt tgccgtcacg gacaatgaac cccgctgcgg tccgatcgga
     3601 aaagttgatg tactccgaat taatgtgggt ataagcgccg gatagccgta gacctgtcac
     3661 aggccgccaa tcggcgaata gttcgacccc gcgataccgc gaagagtctg cattgaccga
     3721 ggcggtgtta tttcccgaga tcacttgcgt gatgatttcg tctttgaaga aggtgtagaa
     3781 gccgaccacg tggacgagca aggttggatg cagcctcatc tccgtgccga tttccagatt
     3841 aaggttcttt tgcggtttta ggctgaaatt gctccccgga agccccgtca caggatctcg
     3901 cagaagattg ctgaattgcg gaatgccata gcctgtcgag gctcgcagcc aataccggta
     3961 gtctgctgac ggtttccaag tcagcgatcc ctcaggcgcc caattggaga aggttcgatt
     4021 ggcactgacc cgtgaggcaa gcgtcccgtt ctgatcatag ttcgtggcat ccacgctgag
     4081 acgggattgt tcgaagccga gcccgactgc caagatccaa ttcgggtaaa actccaattc
     4141 ctcccggata cgaccgccaa tgttgaagat agttccgcgg ctgttctgaa tcaaattccc
     4201 tttcgttccg aaaccatccg caagattctg aaaggtgttg cccttctgct ccatcttatt
     4261 cacaaagaaa ccgacatagc ttttcaacgg catcactccc agccgaccgt catgccgcaa
     4321 gtcagcatag gacttgtagt tgggattcgt attttcgaag atctgcgaaa atgattgctg
     4381 gatgtctttg acgtcataat cggcttccac cgtcaggacg gtattcgcat cgatctgccg
     4441 ctcgtatatc cctccgataa tcgtgcgacg atcgacccgg ctttgatcca gcaacagcgc
     4501 atcgg
//
LOCUS       DATMTI010000612         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_490315, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000612 DATMTI010000000
VERSION     DATMTI010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490315"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VN039_09740"
     CDS             complement(<1..1049)
                     /locus_tag="VN039_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS domain-containing protein"
                     /protein_id="HWV46283.1"
                     /translation="MSDELKVLGLFRDEAALRDHIADHLDDLELGLVVTKTEYPLNNP
                     DGAGGRVDILALDEFDHVVVIELKRSDLSARATLHELAKYVTLLSTEARVPREAIRCI
                     VLSTHWHELLLPLSFFAATSGVHVEGYKLVRADERVAYTAVELVPISDLPQFSPEFSV
                     YQYLTLADRDLHMASIKGRATELPFVRLVFVLLDAPKGSLQTPFRSIVGMWRLKPDDE
                     GELVRMTGHEVGYLFPYAFPGWEAECDALYWIADEERPGVAVSSASEERGTPEKVRNL
                     FSTYECAGVEHIGDWPKIDLINTNERLLRQIEAVSSLSGVDRPNLYTFDQRVTPRFRP
                     SWRRAVQAFLDFIAF"
     gene            complement(1137..>1979)
                     /locus_tag="VN039_09745"
     CDS             complement(1137..>1979)
                     /locus_tag="VN039_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46284.1"
                     /translation="IADFANPRVRLESRFGGFELAFDFQGRIFRQEVETDARRYVAYG
                     AWLRLQLPGQAVDLGYLRPLVPVVMQQLTHETICNFSLAMDCHASQIEIVETYRNGGG
                     LELSLQIHGLSSRPPIAFDREETQSTPAYLSVPQSDWIKQLNRSGAGMVMLSEIHFPS
                     VDRNALAHPGRKHLLDAQTKFLAGDWTGCVAECRKVAEAFGDRKLGKIKASFIKESGI
                     MPKTDRLTMLLVAVEHCAHVAAHATDGSPQEDFTRSEALLLLRLTSSYVMWLAEYENN
                     AKEA"
BASE COUNT          456 a          561 c          541 g          421 t
ORIGIN      
        1 tcgaaagcga tgaaatctaa aaatgcctga accgcccgtc gccaggaggg gcggaagcgc
       61 ggcgttacgc gctggtcaaa cgtatagaga ttgggccgat ctacccctga taggctcgac
      121 acagcttcga tttggcgcag caggcgctca ttggtgttga ttaggtctat ttttggccag
      181 tcgccgatgt gctccacgcc cgcgcactca taggtgctga atagattgcg caccttttcg
      241 ggcgtgccgc gctcctcact ggccgatgaa accgctacgc ctggcctttc ctcatcggcg
      301 atccaataaa gcgcatcgca ctcagcctcc catccgggaa aggcgtacgg gaaaagatag
      361 cccacttcgt ggcctgtcat ccgaacgagt tcaccttcat catctggttt gagccgccac
      421 ataccgacga tggaccggaa cggcgtctgt aacgatccct tgggagcgtc caacaggaca
      481 aatactaggc ggacgaaggg cagttcggtg gctcgtcctt taatgctggc catgtgaagg
      541 tcgcgatccg cgagcgtcag gtattgatag actgaaaact ccgggctgaa ttgtggtagg
      601 tccgagatcg gtaccaattc gaccgccgta taggccacgc gctcgtccgc cctgacgagc
      661 ttgtagcctt cgacatgaac gcccgatgtc gcggcgaaga aagacaacgg cagaagaagt
      721 tcgtgccaat gggtcgataa aacgatgcag cgaatagctt cgcgcggaac ccgcgcctcg
      781 gtcgataaca gcgtgacgta cttggccagc tcgtgaagtg tcgcccgtgc gctgaggtcg
      841 ctgcgcttca actcaatgac aacaacatgg tcgaattcgt caagcgccaa gatgtccacc
      901 cgcccgccgg cgccgtccgg attgttcagt ggatattcgg tcttggtcac gacgagcccg
      961 agttccaggt catccagatg gtcagcgatg tgatcgcgca gagccgcttc atcgcgaaat
     1021 aagcccaaga ccttcaactc gtccgacatc tgtatctcct ggtgcagctc gatacgctct
     1081 atctggggcc tctgatgagc tgcaaaagag cctgacaagt gttatctttg caaaccctat
     1141 gcttctttgg cattattctc gtattcggcc agccacatca cgtagcttga cgtaagtcga
     1201 agcaacaaca aggcctcgga tcgggtgaaa tcttcttgtg gcgacccatc cgtggcatgg
     1261 gcggcaacgt gagcacaatg ttcgacggca accagaagca tagtaagccg gtcggtcttt
     1321 ggcatgatcc cggattcttt aataaacgaa gctttgatct ttcccagctt ccggtccccg
     1381 aacgcttcgg ccacttttcg acattctgct acgcagccgg tccaatcgcc cgctaaaaac
     1441 ttggtttgag cgtccaacag atgcttccga ccgggatgtg cgagggcatt tcgatcaacc
     1501 gaagggaaat ggatttcaga caacatgacc atgccggcac cagaacggtt cagctgctta
     1561 atccagtctg actgggggac ggataagtaa gcaggagtgc tctgagtttc ttcccggtca
     1621 aaggcaatcg gcggccgaga actcaagcca tgaatctgaa ggctgagctc aagccctcct
     1681 ccgttacggt aggtttccac aatttcaatc tgcgaagcgt ggcagtccat agcgaggcta
     1741 aaattacaaa tagtctcgtg cgtgagctgt tgcatcacca cgggtacgag cggacgcagg
     1801 tagcctagat cgaccgcttg tccgggtagc tgcagcctga gccaggcgcc gtaggcgaca
     1861 tagcgtcgcg catccgtctc cacctcttgc cgaaaaattc gcccttggaa atcgaaagcc
     1921 aactcaaaac cgccaaagcg cgattccaat ctgacacggg ggttggcaaa gtcggcaat
//
LOCUS       DATMTI010000613         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_193743, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000613 DATMTI010000000
VERSION     DATMTI010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193743"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VN039_09750"
     CDS             <1..221
                     /locus_tag="VN039_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46285.1"
                     /translation="IAAGVTYMTTWVVFTDWLRLSGGKILLGAVSGLLAAAVWWAIAV
                     HAENAFLQAAVAGLCFGAAYAWSDQRMT"
     gene            complement(206..1531)
                     /locus_tag="VN039_09755"
     CDS             complement(206..1531)
                     /locus_tag="VN039_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWV46286.1"
                     /translation="MGNAVTQIRRSVMILALPVTLTTFLQRAEGIVAVLLVGGLGATS
                     IAAVGLGQLLAFVATTLVSGISVGTNVIVAQLWGARRRQDAGEAARHFLWLSIGVSLM
                     LAGLGIAGNQFVMQQLGAESAVIELALPYSTVIFLVIPCTVLIHVLSSILQGTGDTKT
                     PMYGLIGVNLLHVLLAYPLIYGRWGAPHLGLKGAAIAVGLAEATGTLYLLLRCRPILK
                     ESSTLRLDLIRSIWEVGSSVSGERIVQQAGIFIYTKLVLLYGTVAYAAHQVGLSIESF
                     SFLPGYGLAIAAATMVGQSIGAGKYTRAKLENWEANRLAIVIMACMGLIFFFFPYPLL
                     RAFTTDEAVIELGTMFLQIVALLQVPLALTMVLAGSLRGAGDTRFIMGATMIGMWGVR
                     VPFALIAALWMRQSVFYIWTAMVADWTVRMGLLLWRYQSERWRQIQVIR"
     gene            1605..3125
                     /locus_tag="VN039_09760"
     CDS             1605..3125
                     /locus_tag="VN039_09760"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HWV46287.1"
                     /translation="MADVHNGFPTKVAPPRLSKSKYLSGLQCHKRLYLEIHQPALATP
                     PDASTQAILDMGIEIGILAQRRFRGGVLVKSGFRQRDAAITETAALLQDSGILAIFEG
                     AFEHEGVLVRVDILERMQNGDGGSSSWRLIEVKSSTRVKDVHLDDLSIQSHVVQGAGV
                     RLDATCLMHIDTGYLYQGGEVDLQALFSIEDVSEAVTNRRGRVPERLTAMKAMLLESQ
                     APVIEPGQHCHAPYECPFWAHCTKEKPQRWIYHLPGKKEIVGQLVRQGIMTIDEIPDE
                     TMLSDVQRKVKNNVEWVSPELGHILRSVSYPIHHLDVETVMLALPRFPSTRPYQSLPV
                     QWSNHIELESGEVMHQEFLHNEASEPRKRWAEALIESLGEKGSIVVYSAYEEAIIRQL
                     AETFPEFKSVFKAIVKRLWDLLPVIKSHYYHPAFHGSYSIKSVLPAVVPALGYGDLAI
                     QEGGQAAAEYYRMVFLESDWVERDSIREALLRYCARDTLAMVELRRVLREKAEHHA"
BASE COUNT          858 a          931 c         1054 g          827 t
ORIGIN      
        1 ctattgccgc cggggtgacc tatatgacca catgggtcgt ctttacggac tggcttcggc
       61 tttcgggtgg caagatcctt ctcggcgcgg tctcaggctt gctggcagcc gcagtgtggt
      121 gggcgattgc tgtccatgcg gagaacgcgt tcctccaggc ggcggtggcg ggcctctgtt
      181 tcggagcagc ctatgcttgg tctgatcaac gaatgacctg aatctgacgc catcgttccg
      241 attgatatcg ccagagtaac aaccccatcc gcactgtcca gtcagcgacc atcgctgtcc
      301 agatataaaa gacggactga cgcatccaga gggcggcgat cagtgcaaac gggacgcgga
      361 cgccccacat gccgatcatc gtggcgccca taatgaaccg tgtgtcgccg gcgccacgga
      421 gcgagccggc caggaccatg gtgagagcta gcgggacttg taataaggcc acgatttgta
      481 aaaacatcgt tccgagttca atcacggctt catcggtggt gaaggcacga agcaatgggt
      541 aggggaagaa gaaaaagatg aggcccatac aggccatgat cacaatggcc agccgattgg
      601 cttcccagtt ttccagtttc gctcgggtat acttgcctgc tccgatgctt tgccccacca
      661 tggtggcggc tgcgatggca agtccatagc cgggtaggaa ggagaaggat tcaatcgata
      721 gcccgacttg atgcgcggca taggccacgg tgccatacag gaggacgagt ttggtgtaga
      781 taaagatgcc ggcctgttga acgatgcgtt cgccggacac cgacgatccc acctcccaga
      841 tcgatcgaat caagtcaagc cgcagagtgg aggattcttt caaaatgggg cgacagcgca
      901 gcaagagata gagcgtcccc gttgcttcgg cgagtccgac agcgatggct gctcccttga
      961 ggcccaaatg aggagctccc catcgtccgt agataagagg gtaggcaagt aagacatgaa
     1021 gaagattgac gccgatcagg ccgtacatgg gggtcttcgt atcaccggtg ccttggagga
     1081 tggacgacaa gacgtggatg agtacggtac agggaattac gaggaagatg actgtcgaat
     1141 aaggaagggc gagttcaatg acggcggatt ccgcaccgag ttgctgcatg acgaactgat
     1201 tgccggctat tccaaggccg gccagcatga gcgagacgcc gatcgaaagc cataaaaagt
     1261 gacgggccgc ttcacccgcg tcttgtcggc gccgcgctcc ccataattgg gcgacaatca
     1321 cattcgtccc gaccgaaatt cctgagacta aagtcgttgc cacgaaagcc aacagttgac
     1381 ctaggccgac cgcggcgatt gacgtggctc ccagcccgcc gaccaagagg acggcgacaa
     1441 tgccttcagc tcgctggaga aatgtggtga gagtgacggg gagtgccaaa atcatcacgg
     1501 accgtctgat ctgggtgaca gcgttgccca tggggcgcta tcctaacaga gttcctcccg
     1561 gtaccagatg agaaatcgtt gctcgcaccg tggattgtat gacgatggcc gacgttcaca
     1621 atggatttcc cacgaaggtt gcaccgcccc gcctatcgaa atcgaaatac ctctcgggat
     1681 tgcaatgtca caagcgcctc tatctggaga ttcatcagcc tgcattggca actccgccgg
     1741 atgcgtcgac gcaggcgatc ttggatatgg gaattgaaat cggcattctt gcgcagcgac
     1801 gctttcgggg tggtgtgttg gtcaagtcgg gattccgtca gcgggatgcg gcgatcacag
     1861 agactgcggc cttgcttcaa gattccggga tcctcgccat cttcgaagga gcattcgagc
     1921 atgaaggagt cttggtgcgc gtcgatattt tagagcgcat gcagaatggt gatgggggat
     1981 cgtcgtcctg gcgtttgatc gaggtgaagt catccaccag ggtgaaagat gttcaccttg
     2041 atgacctctc catacaaagt cacgtcgtac aaggagccgg cgtgaggctt gatgcgacct
     2101 gtctgatgca tatcgatacg ggatacctct atcaaggcgg ggaggttgat ctgcaggcac
     2161 tcttttcgat tgaagacgta tcggaagctg tgaccaatcg tcgagggcga gtgccggaac
     2221 gattgactgc tatgaaagca atgttgttgg aatcgcaggc tccggtgatc gagcccggtc
     2281 agcattgcca tgctccctac gaatgtccct tctgggctca ttgcacgaag gaaaaaccgc
     2341 agcgctggat ctatcatctg ccgggcaaga aagaaatcgt cggtcagctt gtccgacagg
     2401 gcatcatgac gatcgacgaa atcccggatg aaacaatgct gtcggatgta cagcgaaagg
     2461 tcaaaaacaa tgttgagtgg gtttcaccgg aattaggtca catccttcgt tccgtaagct
     2521 atcccatcca tcatctcgat gtcgaaaccg tcatgttggc gctgccacgg tttccctcca
     2581 ctcgacccta tcagtctctt ccggttcagt ggtcaaacca cattgaactc gaatccggtg
     2641 aggtcatgca ccaggagttt cttcacaatg aggcatcgga gccgcgcaag cgttgggccg
     2701 aggccttgat cgagtcgctc ggcgaaaaag gcagcatcgt tgtgtattca gcctatgagg
     2761 aggccattat acgccaactt gcagagacgt ttcctgagtt caaatccgtg ttcaaagcga
     2821 ttgtgaagcg tttgtgggat ctgctccctg ttatcaaaag tcactattat catcccgcct
     2881 ttcatgggtc gtattcgatt aagtctgtgt tgccggcagt ggtgccggcg cttggatatg
     2941 gcgatctcgc gattcaagag ggcgggcaag cggcggctga atactaccgg atggtctttc
     3001 tcgaaagcga ttgggtggag cgggattcca tacgcgaggc attgttacgc tattgcgcgc
     3061 gagatacgtt ggccatggtc gaactgcgga gagtattgag agaaaaggct gagcaccatg
     3121 cctgagtaca aggtgatgag tggtggacca agagagggaa aaacaactgg tggggtttat
     3181 ccgctcgcga taattgcgat accttatttg aaccactttt ttgcgcttgg cctacgatga
     3241 gggcaacctg gccaacaaca cggccgacgc tttcctttac gcaggtcttc acaaaaacgt
     3301 ttcaagtgtt cttgtcgtgt ctcatctttc ttcaccatag tcacccaaca aacccactca
     3361 ttgcgagcga gaggtgtaag cctgttccat aggtgtaata catcaggatt ggaatttagt
     3421 acttcttgca gatcttttgg tactttgtga aagaagctgg tgctgatgac ttgagtaggc
     3481 ataacatcta aattatacag aatttgctta ccattcacga ccaccccaca ttggtcgtcc
     3541 ccatgatgcg cttcgcgacg actccatttc agcttcagat gccaatccta atttttgtga
     3601 agagggtagg aattcgaagt tgcgctcgca ggggttgctg acgctcctaa tcaatggtag
     3661 acagcccatt
//
LOCUS       DATMTI010000614         1799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_830094, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000614 DATMTI010000000
VERSION     DATMTI010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830094"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VN039_09765"
     CDS             complement(<1..536)
                     /locus_tag="VN039_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HWV46288.1"
                     /translation="MPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAE
                     KKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKM
                     GVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRR
                     HWGGNVLGPKSVARIAKG"
     misc_feature    complement(531..1084)
                     /note="23S ribosomal RNA rRNA prediction is too short"
     gene            complement(1529..1604)
                     /locus_tag="VN039_09770"
     tRNA            complement(1529..1604)
                     /locus_tag="VN039_09770"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1569..1571),aa:Ala,seq:tgc)
     gene            complement(1636..1712)
                     /locus_tag="VN039_09775"
     tRNA            complement(1636..1712)
                     /locus_tag="VN039_09775"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1676..1678),aa:Ile,seq:gat)
BASE COUNT          379 a          531 c          461 g          428 t
ORIGIN      
        1 atccccttgg cgatacgagc cacagactta ggacccagga cattgccacc ccagtgacgg
       61 cggatctcat cgtatctgtc attgtaattg gtcctgatag cttccaccag cttagccaaa
      121 gcgcctttgt cttccgagtt cacctgtgtg aaggcgacag tggtgcaggt cttcctgtgg
      181 actagacgtc ccagtcttgc ctttcccttg ataatgcagt aagggacccc cattttacga
      241 cacagggcag gcaagaagac aaccagctcg atgggatcca cgtcgtgtgc aatcaccacc
      301 agctgagctt tcttgttctc caccaaggtg gtgacggtgt taactcctgc tcgaaggaca
      361 ggtggtctct tcgttgggac gtcccctttg ccagcagcct tcttctcggc ccgggccaac
      421 agtctctgct tcttctcttg ctttgtctct ggtctgtact tgtgggccag cttaagcagc
      481 tgagtagctg tttggcggtc cagggcctgg gtgaactggt taatcgcagg aggcactttc
      541 aggtactatt tcactcccct cgtcggggtg cttttcacct ttccctcacg atactggtgc
      601 actatcggtc ggtaaggagt acttaggctt ggagggtgat ccccccatgt tcaaacagga
      661 ttgcacgtgt cccgttctac tcgaggataa agctctgcat tacctgtacg gggctgtcac
      721 ccgctgaggc gcgtcattcc agacgcttcc ggttatctct ggattgtcac tggcctggtc
      781 cgctttcgct cgccactact agcggagtct cggttgatgt cctttcctcc gggtactgag
      841 atgtttcagt tctccgggtt agcttaaaac cccctatttt attcaagggt cttatccttc
      901 catttgataa cggaaatcca agacacacgc tcttgcgagc gggcggctca gaattccgtt
      961 atcggaagtg ggtttcccca ttcggaaatc cgcggatcaa agcttcttcg cagctcctcg
     1021 cggcttatcg cagcgtagta cgtccttcat cgcctctcag cgccaaggca tccaccaaat
     1081 gcccttacga cacttgatcg ctctcattat caatgcccat ccatcatccg ttcgtcgtct
     1141 ggatcctcgg cgaatcccgg cgacaagata tgtaacggcg atggccgata cgaatttgca
     1201 ttcgcatcga tagaacactg atcagaaaga ccattctttg cttcaagatc gaaccgacag
     1261 cgaggcggtc aagcttcact gacatgcctt gccgccgcca gccgatggat gaaggccggc
     1321 gtcaaacaag acattaggag tgcgcaaaac gtttccgcga cgtgagatcc agcgatctca
     1381 tgtcgttttc acgttcgctc ggaacgatct cctcttcacg atgtcagaca acacgcgaca
     1441 agccggcgtc ccgtccacgg gacacgtacg cttgcgcgaa tttgttttct tacggacgag
     1501 cagcaacagg ccgatcggga tcgccgactg gtggagccag acgggatcga accgacgacc
     1561 tcctgcttgc aaagcaggcg ctctcccaac tgagctatgg ccccgaaagt cacggcaccc
     1621 aggaaaagcg ggaaatggtg ggcctgggaa gatttgaact tccgacctca cgcttatcaa
     1681 gcgcgcgctc taaccaactg agctacaggc ccaagaagcg cacgcttgac cccgcgcatc
     1741 caacaggacg gagggcaaaa ggatgctcca acgaccatac ggaagcgtgg gctcgtccg
//
LOCUS       DATMTI010000615         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_193775, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000615 DATMTI010000000
VERSION     DATMTI010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193775"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VN039_09780"
     CDS             complement(<1..755)
                     /locus_tag="VN039_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV46289.1"
                     /translation="MQPITSWWSQRLTVSRATDNLMSDGGTVERNVVTGAVGPIGFPF
                     RSQIETTSNRIEWQHNVQVGKPLLLTAGYQFREQIGSNTDLLTQTATFSNKTLSSHAG
                     FGEAQLNLWDRVFGTAGIRYDDYNVFGSATTYRVTGGYLHHETGTKLRGSYATGFRAP
                     TINQLFFPGFGTPNLQPEKSQSFEAAIEQTLPNDRGSISVGYFWTRYRNLILSVFDPA
                     VCTAPGSFGFCAQNAGLARAQGVEVSTKVKLFR"
     gene            complement(789..1637)
                     /locus_tag="VN039_09785"
     CDS             complement(789..1637)
                     /locus_tag="VN039_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWV46290.1"
                     /translation="MLGFVARRVCRACVLLSWIVILAYPASAQEVAMADQPELEAPDV
                     VVSATKTPTPVKQVTSAVEVITGEQMQQRKIRTISEALRWAQGLSVNQSGGPGTAVDV
                     RIRGGTPEQTMVLIDGAIVNSATIGNYDFANLTSDNIERIEILRGSQSMLWGSDAMGG
                     VINITTKRGRDKPNISGFAEYGSFNTMREGASLAGKKGPIDFSGSITRWDTAGFSAIN
                     YRRGAAERDGYHNWQGSLRLGADLPKDGRIEFSFRWLNSIVNFDGFAFNPVTFASDPA
                     DVFGAK"
     regulatory      complement(1662..1855)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1977..2744)
                     /locus_tag="VN039_09790"
     CDS             complement(1977..2744)
                     /locus_tag="VN039_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWV46291.1"
                     /translation="MRTGTIALLHLETVPGAIERNRYVIVEAIKHAAGKGAEWIVTPE
                     LAVCGLQFAHAVGSDWIQPQPDPWMQQVCKLVKTLKRTVFLGCPERHGKRFYNSVFVI
                     GPAGEILGKHRKINVVSDSLSWSSPGDIVAPIECDGIKVGVLICSDIYTSNIARALTF
                     EGAQIFVSSASWGPGIHGPSGEWEQRTHETGLPLIVCNRTGSEKTLDFWNAPSLVVQN
                     GTRLLAHTSKKSAVLTFNWDFDGMRLRSSKYSIDYMR"
     gene            complement(2746..>3035)
                     /locus_tag="VN039_09795"
     CDS             complement(2746..>3035)
                     /locus_tag="VN039_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV46292.1"
                     /translation="DLQHQVDICRVIRQLKEEQGLTIVLVSHDLNLVSQYCDRILLLD
                     RGRVIRLGCPEEVIEPEALEAVYRCRALVDRHPGTGLPRVTLPGRSLSGRT"
BASE COUNT          651 a          893 c          775 g          716 t
ORIGIN      
        1 tcacgaaaca atttcacctt cgtgctgact tctacgcctt gagccctggc caatcccgca
       61 ttctgcgcac agaacccaaa cgaacctggg gccgtacaga cggccggatc gaataccgac
      121 aagatgagat ttcgatatcg agtccaaaag tatcccacgc tgatcgaacc ccgatcattg
      181 ggtaatgttt gttcgatggc cgcttcgaac gattggctct tctcgggctg caagttcgga
      241 gtcccgaagc ccgggaaaaa taattgattg atcgtcggtg ctcgaaatcc tgtcgcatag
      301 ctgccgcgta gcttcgtacc cgtttcatga tgcaggtacc ctccggtcac acggtaggtt
      361 gtggcgcttc cgaagacgtt gtaatcgtca taccgaatac cggcggttcc gaacacgcga
      421 tcccacaggt tcaactgggc ctctccaaat cctgcgtggc tgctgagggt cttgttcgaa
      481 aaggtagccg tctgagtgag tagatctgta ttgctgccta tctgttctcg aaactgatag
      541 cctgccgtca gcaataacgg tttgccgacc tgaacattgt gctgccattc aatccggttg
      601 ctcgtcgtct cgatttgaga cctgaagggg aacccgatcg gtcctaccgc gccggtcacg
      661 acgttacgtt ctaccgttcc tccgtcgctc ataagattgt cggtcgctcg tgacaccgtc
      721 agccgttgcg accaccatga ggtgatcggc tgcatgtaat ttcccgcaaa aatgtattgg
      781 ctgtccttct atttcgctcc aaagacatcc gccggatcgg atgcgaaggt caccggatta
      841 aaagcaaatc catcaaaatt cacgatgctg ttcaaccatc gaaagctaaa ttcgatcctt
      901 ccatcctttg gaaggtccgc gccaagccga agagatcctt gccaattgtg gtatccatca
      961 cgttcagccg ccccacgtcg gtaattgatc gccgaaaagc cggccgtatc ccaacgggtg
     1021 atcgatccag agaaatctat aggacccttc ttgccggcca ggctggctcc ctctcgcatc
     1081 gtattgaaag agccgtactc tgcaaacccg gagatgttgg gcttgtcacg tcctcgcttc
     1141 gtcgtgatgt tgataacccc acccatggca tctgagcccc aaagcatgct ctgacttcct
     1201 cgcaagatct cgatccgttc aatgttgtcg gacgtgaggt tggcaaaatc ataattgccg
     1261 atcgtggcgc tattgacgat cgccccgtcg attaagacca tcgtctgctc cggcgttcct
     1321 ccgcgaatcc ggacgtccac ggccgtgccg gggcctccgc tctggttgac ggacaatcct
     1381 tgagcccagc gcagagcttc tgaaatcgtt ctgatttttc gctgctgcat ctgttcgccg
     1441 gtgatgacct cgactgcgct ggtgacttgt ttgacagggg ttggggtttt ggttgcactc
     1501 acgacgacgt ccggagcttc gagctccggc tggtcggcca tcgcgacttc ctgagctgag
     1561 gcgggatagg cgagaatcac gatccatgag agaaggacac aggcacgaca aacacgacga
     1621 gctacgaacc ccaacatgga aacctccctt tgactcgaag ggtattgata atcccggcag
     1681 ttgggtctcc tgacttgcgg ctcgtcgcgt ctctgcgcct tcccatgtgc cgaggcacac
     1741 agtggcatcc tgcagagtca ctccccgctt acagtggcgg caccgtgatg gatttgcacc
     1801 atcttccccg tcgctgtcgg tgtgatctta acgaatcccc ctagaataat tgttgcgcac
     1861 ccagtttcct atgcaggaga ggggtgcttc tcttcctgcg tacatcatgc tgcattggtt
     1921 aagcgaacgt tcgctactcc accaagcttt catctccacg atcacgtgtg atctcactat
     1981 cgcatgtaat cgatggaata ctttgaggaa cgtagcctca tgccgtcgaa atcccaattg
     2041 aatgtcagta cggcggattt cttggaggta tgtgcgagca gacgcgtgcc attctgtacg
     2101 accaggctcg gagcgttcca gaaatccagg gtcttctctg agccggttcg gttacagacg
     2161 atcagcggga gtcctgtttc atgggtccgc tgctcccatt caccactcgg accgtgaatg
     2221 cctgggcccc acgatgccga cgacacgaag atctgtgcgc cttcaaatgt aagtgctcgg
     2281 gcgatattcg acgtatagat gtcgctgcag atgagtaccc ctactttgat cccatcgcac
     2341 tcgatcggag cgacaatgtc gccgggactc gaccatgaca gcgaatcgct taccacattg
     2401 atcttgcgat gtttacccag gatctcgccc gccgggccga tgacaaacac tgagttgtag
     2461 aaccgcttgc cgtgccgctc cggacacccg aggaatacgg ttcgtttcaa tgttttgacc
     2521 agcttgcaga cctgctgcat ccaaggatca ggttgcggct gtatccaatc agagccgacg
     2581 gcatgtgcga actgaagccc gcagaccgcg agttccggtg tcacgatcca ctcggcacct
     2641 tttccggcgg cgtgtttgat cgcctcgaca atcacgtacc ggttccgctc gattgccccg
     2701 ggaaccgtct caagatgaag cagcgcgatc gtgcccgttc tcatatcacg ttctaccgga
     2761 gagggaacgc ccaggaagtg tcacccgagg caaaccggtc cccggatgtc gatccaccaa
     2821 tgcccggcat cgatacacgg cttccaatgc ctccggttca atgacctcct caggacaacc
     2881 aagccgtatc acccgtccgc gatccaacaa cagaattcgg tcgcaatact gactcacaag
     2941 attcagatca tgggatacca gaacaatggt cagcccttgt tcttccttga gctgtcgtat
     3001 aacccgacag atgtcgactt ggtgttgtaa atcga
//
LOCUS       DATMTI010000616         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_616921, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000616 DATMTI010000000
VERSION     DATMTI010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616921"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VN039_09800"
     CDS             complement(<1..641)
                     /locus_tag="VN039_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWV46293.1"
                     /translation="MPDARPPLITLLTDFGERDSFVASMKGVILSINSYAAVVDLSHQ
                     IASHQIQEAGYFLKSCYRYFPEKTIHVAVVDPGVGTERRALLVSAAGSFFVGPDNGLF
                     TEVLEQEVGAKVWQINSPQYRLETVGSTFDGRDVFAPAAAWLSKGVPPSFFGPAVHDP
                     IRRSVATPVWHEELLIGKIISVDRFGNLISNITARQVREFRSAMGQTVEIHIGT"
     gene            complement(643..1119)
                     /gene="greA"
                     /locus_tag="VN039_09805"
     CDS             complement(643..1119)
                     /gene="greA"
                     /locus_tag="VN039_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWV46294.1"
                     /translation="MPTPITKKGYDALKAELDRLRKVERPKVIEAIAEARAHGDLSEN
                     AEYDAAKERQGFIESRLSELETKLADARVVETAGRTTETVVFGATVLVVEQESQSRKQ
                     YTLVGQDEADMKFNKISVQSPVGRALIGKRVGDFVEVKTPVKLVEYEVVEIKFEDF"
     gene            complement(1142..>3509)
                     /gene="carB"
                     /locus_tag="VN039_09810"
     CDS             complement(1142..>3509)
                     /gene="carB"
                     /locus_tag="VN039_09810"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWV46295.1"
                     /translation="VVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDEITNDI
                     TGVTKASFEPAIDYVVVKIPRFAFQKFKGADPTLTTQMKSVGEVMAIGRTFKESLQKD
                     ISSLELELNGLASRFGLDRGVPAEFNRPEAIEKLSRILRIPLPERLWYVADAMRLGFT
                     DEELFATTKIDPWFLEQVRQLVTFERLLAGHAADPAAVLGSELLWDAKELGFSDDRIA
                     QLLGCEAAAVQRGRTQQGTRGVTYKRVDTCAAEFEAQTPYLYSTYGMECEARPSDRQK
                     VVILGGGPNRIGQGIEFDYCCVHAAMALREESIDTIMVNCNPETVSTDYDTSDRLYFE
                     PLTHEDVLNIVHREQPIGVVLQFGGQTPLKLALPLSKAGVRILGTSPDAIDLAEDRER
                     FRDLLNRLGLRQAESGTARSVEEAVQIAGRISYPVMVRPSYVLGGRSMQIVYDETGLL
                     EYMHSAVKASPNHPVLIDKYLADAIEVDADAISDGDTVVVAGIMEHIEEAGVHSGDSA
                     CSLPPYTLDKPIVRDIERQMRLLAQELGVVGLMNAQFAVKDRTIYVLEVNPRGSRTVP
                     FVSKAIGVPLAKLAMKVMMGKTLKELGFTDAPLPAHFSVKEAVFPFNKFPGVDVLLGP
                     EMKSTGEVMGIDGDFGWAFAKSQAGAGAVLPTSGTAFVSVKQSDRHVALDLSKRLCAL
                     GFQIQATSGTAGYLREHGVEVGTVNKVKEGRPHIVDHIKNGAVALVINTVRTASAHVD
                     SLSIRREALHRGVPYFTTMRGAHAAVMGIEATAQKGLAIRTLQEYHRP"
BASE COUNT          732 a         1070 c          918 g          789 t
ORIGIN      
        1 gtgccgatat ggatctccac ggtttgtccc attgctgatc ggaattcccg aacttgccgc
       61 gccgtgatat tggaaatgag attcccgaac cgatcgacgg agatgatttt tccgatcagc
      121 aattcctcat gccaaaccgg ggtcgccacg gaacgtcgga ttggatcatg gaccgccggt
      181 ccaaaaaacg acggtgggac tcctttgctc aaccatgcgg cagccggtgc gaacacatcc
      241 cgaccgtcaa aggtcgaccc gaccgtttcc aaacgatact gcggactgtt gatctgccac
      301 accttcgccc cgacctcctg ctccagcact tcagtaaaca acccgttgtc cggaccgaca
      361 aagaacgagc cggccgcaga aacaagcaac gcccggcgct ccgtcccgac tccgggatcg
      421 accacggcga cgtggatggt cttttccggg aaataacgat agcaggactt caggaagtat
      481 ccggcttctt ggatctggtg agaggcgatt tggtgtgaaa gatcgacgac ggcggcgtag
      541 gaattgatcg acagaatgac ccccttcatg ctggcgacaa aagaatcacg ctcgccgaaa
      601 tccgtcagca gggtgatgag gggggggcgt gcgtccggca cgtcagaagt cctcgaattt
      661 gatctccaca acctcgtatt cgaccagctt gacgggggtt ttcacttcca cgaaatctcc
      721 gacacgcttc ccgatgagcg cgcgaccgac cggggactgt acggagatct tgttgaattt
      781 catgtcggct tcgtcctgcc cgactaaggt atattgtttc cgcgattggg actcctgttc
      841 gacgaccaac acggtcgcac cgaagacaac cgtctcggta gtacgtcctg ccgtttccac
      901 cacgcgggca tcggcgagtt tcgtctcgag ttcggagagg cgtgactcaa taaacccctg
      961 gcgctctttg gctgcgtcat actcggcgtt ctcgctgaga tcgccgtgag ccctggcctc
     1021 cgcaatcgct tcgatcactt tcggccgctc aaccttgcgc aaacgatcca attccgcctt
     1081 taacgcatca tagcctttct tcgtaatggg ggtcggcatg ctccaatcgc tcctgaacaa
     1141 tttatggccg atgatactcc tgcaatgtac gaattgctaa gcccttttga gcggtcgctt
     1201 caatgcccat caccgccgca tgggcacctc tcatggtcgt gaaatacgga acgcctcggt
     1261 ggagggcctc ccgcctgatg gacaaggaat ccacgtgagc agaggcggtc cgcaccgtgt
     1321 tgatgacgag cgccaccgcg ccattcttga tatggtcgac aatgtgggga cgtccttcct
     1381 ttactttatt gaccgtaccc acttcaaccc cgtgctctct cagatacccg gctgttccgc
     1441 tggtcgcttg aatctgaaac ccgagcgcac ataaccgctt cgaaagatcc aatgcgacat
     1501 gacgatcgga ttgcttcacg ctcacgaagg cagtccccga tgtcgggagg accgcgccgg
     1561 ctcccgcttg ggactttgcg aacgcccatc cgaaatctcc gtcgatgccc atcacttctc
     1621 cggtcgattt catttcaggc cccagcagca cgtcgacccc cgggaattta ttgaacggaa
     1681 agaccgcttc tttgaccgag aaatgtgccg gtagaggcgc gtccgtaaat cccagctcct
     1741 tgagggtttt tcccatcatc accttcatcg ccaacttggc gagcggtacg ccgatcgctt
     1801 tactcacgaa cggtacggtc cgagatcccc gaggattgac ttcaagcaca tagatggtcc
     1861 ggtctttcac ggcaaactgt gcattcatca gtccgacgac gccgagttcc tgcgccagca
     1921 agcgcatttg ccgctcgatg tcgcgcacga tgggcttgtc caacgtatat ggaggcagcg
     1981 aacaggctga atcgccggaa tgaaccccgg cttcttcgat atgttccatg attcccgcaa
     2041 ccaccacggt gtcgccgtcc gaaatcgcat cggcatcgac ttcgatggca tcggcgagat
     2101 acttatcgat caataccggg tgattggggg acgccttgac cgcggaatgc atgtactcca
     2161 gcaagcctgt ttcgtcatac acgatctgca tcgaacgtcc gcccaacacg taggacggac
     2221 ggaccatgac cggataactg atccgaccgg cgatctgcac ggcttcctcg acggatcggg
     2281 cggttccact ttccgcttgc cttaagccca atctattgag gaggtctcga aaccgttccc
     2341 tgtcttccgc caaatcgatg gcatcgggac tggtgccaag aatcctcaca ccggctttcg
     2401 agagcggaag cgcaagtttc aagggcgtct gtccgccgaa ctgtaagacc actccgatcg
     2461 gctgttcccg atggacgatg ttgaggacgt cctcatgcgt cagcggctcg aaatacagcc
     2521 gatccgacgt atcgtaatcc gtgcttaccg tctccgggtt gcagttcacc atgatcgtgt
     2581 cgatcgattc ttctcgaagc gccatcgccg catgcacaca gcagtagtcg aactcgatcc
     2641 cctgtccgat ccgattggga cctccgccga gaatcacgac cttttgtcga tccgacggtc
     2701 tggcttcgca ctccatgccg tacgtggaat agagatacgg cgtctgcgcc tcgaactccg
     2761 cggcgcatgt gtcgacgcgt ttgtaggtga caccccgtgt tccttgttgg gtacgccccc
     2821 gctgaaccgc tgccgcctcg catccgagta attgtgcgat ccgatcatca gaaaatccca
     2881 gttcttttgc gtcccagagc aactcgctgc ccaagacagc tgcggggtcg gcggcatggc
     2941 cggcaagcag tcgttcaaac gtcaccagct gtctgacttg ttctaaaaac cagggatcta
     3001 ttttggtcgt cgcaaacagc tcctcgtccg tgaatcccag gcgcatggcg tcggccacgt
     3061 accacagtcg ttccggtagt ggtatccgta gtatccggct gagtttttcg attgcttccg
     3121 gccggttaaa ctcggctgga acgcctcgat caagtccgaa ccgggacgcc aacccattca
     3181 gttccaactc cagagagcta atgtccttct gcagagactc cttgaaggtc cgtccgatcg
     3241 ccatcacttc cccgaccgac ttcatctgcg tggtcaaggt cggatcggcc cccttgaatt
     3301 tctgaaaggc aaatcttggg attttgacca cgacatagtc gatcgccggt tcaaacgatg
     3361 cttttgtgac accggttatg tcattggtga tctcatccaa ggtgtatccg atggcgagtt
     3421 tggcggcgat cttcgcgatc ggaaatccgg ttgccttcga cgccaatgcc gagcttcgag
     3481 atacccgagg attcatttca atgacaacc
//
LOCUS       DATMTI010000617         4167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_193788, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000617 DATMTI010000000
VERSION     DATMTI010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193788"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(446..2152)
                     /locus_tag="VN039_09815"
     CDS             complement(446..2152)
                     /locus_tag="VN039_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HWV46296.1"
                     /translation="MSTPEPSTSSNFIRDLVAADRAAGKHGGRVVMRFPPEPNGYLHI
                     GHAKSIVLNFGIAHETPGGVCHLRFDDTNPTTEDLEYVQAIQDDVRWLGFDWHDKVFY
                     ASDYFEQLYAFAIVLIKKGKAYVDSLTAGQIREHRGTLTEPGRDSPYRTRSADENVNL
                     FARMRAGEFSDGAHVLRAKIDMGSPNINLRDPILYRIRHAAHYRTGTAWCIYPSYDFA
                     HPLSDAIEGVTHSICTLEFEDHRPLYDWVVAEADTAHRPQQIEFARLNLTSTVMSKRK
                     LLELVDKKLVAGWDDPRLPTLKGLRRRGVTPEAIRAFCEHIGVAKRDAIVEMPLLEHF
                     IREDLNKRSPRVMAVLRPLKVVLDNYPEGVVEELDAVNNPEDVSTGTRKVPFSRTLYI
                     EQDDFKEDPPKQFYRLAPGREVRLRYGYIIKCMSATKDPQTGAVTELHCTYDPETKSG
                     SAQEQRKVKATVHWVSASHAAAATIRLYHPLLLPDQAKLPAEQDWTGSLNPQSLELLT
                     GCLVEPSLRSALPGSRFQFERQGYFCVDSDSSAEALVFNRTVSLKDTWAKVEKAHQAS
                     QR"
     gene            complement(2249..2674)
                     /locus_tag="VN039_09820"
     CDS             complement(2249..2674)
                     /locus_tag="VN039_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46297.1"
                     /translation="MNEIPKPTVQAFLVCDQVIEDSLTKKKSLIGIFTHLQAATFPFQ
                     HQQMGLYFCLTDAEGLYHFDIDLIYLNTEQLVCRATLPNIEIGDRLQVSDFGINIPLL
                     IFPAPGRYEFRLRMEGHLIAQKDFNVTPTRMSDTTEPSA"
     gene            complement(2777..3310)
                     /locus_tag="VN039_09825"
     CDS             complement(2777..3310)
                     /locus_tag="VN039_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46298.1"
                     /translation="MDDSVAFRRTEPSGHTVTAGVLSKPGLELRIGSNIFRSTNGVVK
                     IHGKEQLVLEVKPERGLLLATLDLYNEHGIRLAHLRRNVLMLNDNGRFTVDVLLGQGL
                     SSVDSPSILVTDLQLGHVVLEARMTSEHRVDLVCGKLYSHKGMLVEITPHYCRIGSGT
                     ALFGNIVEARGGPAILG"
     gene            3563..3640
                     /locus_tag="VN039_09830"
     tRNA            3563..3640
                     /locus_tag="VN039_09830"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3599..3601,aa:Glu,seq:ctc)
BASE COUNT          899 a         1081 c         1224 g          963 t
ORIGIN      
        1 ccttcgcgag gtgagcaaaa agcatgacct tagcaagttc attccaactt gccaatagtg
       61 aagagagccg cctctgaaca gtactgatcg ttcactgcgt ggaacgcgtg ggattcaact
      121 cggtaaggtg gaacatgacc ctgttgttcc aggaagcggg cactcggtcg acgagcctcc
      181 ctcaactcct cgttcggcct tcgccgtgat gctcgtctcc agtgagaggc tcgggagtga
      241 ccagctcggt tgtaagcgcc ggacctggaa tacgatattg ttccgcggcc cacgttccca
      301 gatcagtgat ttgacaacgc tcagaacaga atggacgcca ggggtttgct tcccatgtcg
      361 tgggttggtg gcatagggga caggtcatgg gcgtagtgta acgtcggaac cgaatgaagt
      421 acagtcgctg gaacgccggt tgatatcagc gctgggatgc ttgatgagcc ttctcaacct
      481 tggcccaggt gtctttgaga gatacggttc gattaaagac caaagcctcg gctgatgagt
      541 cggaatctac acaaaaatac ccctgacgct cgaattgaaa acgagaacct ggcaaggccg
      601 agcgaaggct tggttcgacc aagcagccgg taaggagttc caacgactga gggttcagcg
      661 atcctgtcca gtcctgttcg gccggaagtt tggcctggtc ggggagaaga aggggatgat
      721 agagccggat ggttgcagcg gctgcatgtg acgccgacac ccagtgaacg gtggctttca
      781 ctttgcgctg ttcttgtgcc gatccgcttt tggtctcagg atcgtaagtg caatgcagtt
      841 cggtgaccgc accggtctga gggtctttcg tcgcactcat acatttgatg atgtatccat
      901 agcgcagccg gacctcccgg ccgggcgcga ggcggtagaa ctgttttggc gggtcctcct
      961 tgaaatcatc ctgttcgatg tacagcgttc ttgagaacgg aacctttctc gttcccgtgg
     1021 agacgtcctc gggattattc accgcgtcga gttcttcaac gacaccttcc ggataattgt
     1081 cgagtacgac cttcaatggt cggagcaccg ccatcacgcg cggtgaccgt ttgttcaagt
     1141 cttctcggat gaagtgttcg agcagcggca tctcgacgat tgcatcgcgc ttcgcgacgc
     1201 cgatatgttc gcaaaaggcg cgaatcgctt cgggagtcac ccctcgtcgc cggaggcctt
     1261 tcaaggtggg aaggcgtgga tcatcccagc ccgccacgag tttcttgtcg accaattcga
     1321 gaagcttacg cttgctcatc accgtggagg tgagattcag ccgggcaaat tcgatttgct
     1381 gcggacgatg ggcagtgtcg gcttcggcaa cgacccaatc gtagagggga cggtgatcct
     1441 caaactccag cgtgcagata gagtgcgtga ctccttcgat ggcgtcagac aacgggtgtg
     1501 caaagtcata cgagggatag atgcaccatg cggttcctgt ccgataatga gcggcatggc
     1561 gaattcgata gaggatcgga tcgcgcaggt tgatgtttgg ggaacccata tcgattttgg
     1621 cgcgtaggac atgggctcca tcggaaaatt ctccggctcg catgcgggca aagagattca
     1681 cattctcgtc ggctgatcgg gttcgatagg gactgtcacg gcctggttca gtgagagtgc
     1741 cgcggtgttc acggatttga cccgctgtga ggctgtcgac ataggctttg cctttcttga
     1801 tcaaaacaat ggcgaaggca tagagctgct cgaaatagtc cgatgcatag aacaccttgt
     1861 cgtgccaatc aaaccccaac cagcggacgt cgtcctgtat ggcttgaaca tactcgaggt
     1921 cttccgtggt gggattcgtg tcgtcgaatc tcaggtgaca gacccctccg ggagtctcgt
     1981 gcgcgatccc aaaattcagc acgatggact tggcatggcc gatgtggaga tatccattag
     2041 gctcgggagg aaatcgcatg acgacccgtc cgccgtgttt gcctgccgcc cggtcggctg
     2101 ctacgagatc acgaatgaaa ttcgaagagg ttgagggctc aggtgtgctc atgaagtcct
     2161 tcgtgaatcc ttggcacggt catgtgtgga caggaggtcc agactggagt tctaccatag
     2221 acgggccgat gtgaggaatg ggaaacggct aagcagaggg ctcggtggtg tccgacatcc
     2281 gtgtcggcgt gacgttgaaa tctttttggg cgatgagatg gccttccatg cgcagcctga
     2341 attcataccg accaggtgcg ggaaagatca acagaggaat gttgatcccg aaatctgaaa
     2401 cctggaggcg atcgccgatc tcgatgttgg gcagcgtggc ccgacagacc agttgttcgg
     2461 tgttgaggta gatcaagtcg atatcaaagt gatacaggcc ttcggcatcg gtgaggcaga
     2521 agtagaggcc catctgttga tgctggaacg ggaaggtagc ggcctgtaga tgggtaaaga
     2581 tcccgatcag gctcttcttt ttcgtgaggc tgtcctcgat cacctggtcg cagacgagaa
     2641 atgcctggac ggtaggtttt gggatttcgt tcatagtcgt cattttaggg aaaggaggga
     2701 taagggtaaa acggttctcg cttttctgca gagatgaaaa ctcaggagtc ggagagtatg
     2761 tgacagacga acaggatcaa ccgaggatgg ccggcccgcc tcgtgcttcc acgatgtttc
     2821 cgaacagcgc cgtgcctgag ccgatccggc agtagtgcgg agtgatctct accagcattc
     2881 ccttgtgcga gtacaacttt ccgcagacga gatcgactct atgctccgat gtcattcgtg
     2941 cttcaagcac gacgtggccc aactgcagat ctgtcaccag aatagaggga ctgtcaaccg
     3001 acgaaagacc ttggcccagc agaacatcga cggtgaatcg tccgttgtcg ttcaacatga
     3061 gaacgtttct acgcaggtgt gcgagtcgga tgccgtgctc gttatagaga tcaagggtgg
     3121 cgagaagcag tcctcgttcc ggtttgacct caaggacgag ttgctctttc ccatgaatct
     3181 tgacgacgcc attggtgctg cgaaaaatat tcgaaccgat acggagttcg agtcccggct
     3241 ttgaaaggac gccagccgtt actgtatgac cagagggctc ggttctgcga aacgccaccg
     3301 agtcatccat tttaacaacc tcgaaccatg aggttcttag gtcgtgtgga ggtgcaaggc
     3361 atatggagct tcattaaaac gttagttaag ctaggcgttc cgctctgccc ctgtcaagca
     3421 cgtccaatgc ggaaagccat atattatggc tccgtgcaaa cggcctaatg ggacgatccc
     3481 aaagtgctct ccttccggag gatcggcgct tcgttgcaaa cggaaaagcg cttatgctac
     3541 ggtccactcc tcggtctgga atggtcccat cgtctagcct ggcctaggac ggagccctct
     3601 caaggcttaa acacgggttc gaatcccgtt gggaccacca cacttttcct tcatctctcc
     3661 gtgagttagc caccttcgtc tctgcggtcg cctcctccgt cggaattcca taggacaatc
     3721 cataggacaa aagggcgttg tagaggcgag tcacggcttc acggagctgc tgattccagc
     3781 cgtatccgcc gtgcgaatag gcagccgtca ttgattctcc gccgagctga tcatctccga
     3841 ttcctcgcag tcgatgcccc aataatgcgg cacggacctc gagcgggacc ccgaggttct
     3901 ggagccagcg ggaatcgacc cttctctgca agcggtctcc aatcgtgcca tttcttctgc
     3961 atgcctcgac tgcataggct ctgggatgct gtcagttggt tctttgcgtg ggcccgcgct
     4021 ctggccacaa agatctgctt tcgaatttgg ttttttcttt ggcatgtccc ctcctatgga
     4081 ggccctggtg aagagaaggg gcatgggcgc caggaaacgc ccgccggctg gttcatgagg
     4141 ccagccattg cccttgtgaa ccgctac
//
LOCUS       DATMTI010000618         7268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_830152, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000618 DATMTI010000000
VERSION     DATMTI010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830152"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1268
                     /locus_tag="VN039_09835"
     CDS             246..1268
                     /locus_tag="VN039_09835"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWV46299.1"
                     /translation="MPTTINKIGVIGAGAWGTALAKHLAEKGLEVCLWAYEQDVVNAI
                     NSSHENSVFLKNVPLPPGLTATSSLVEAVKSRDGVLFAVPSHLTRSVLHQLAPCIPGS
                     LPFVCATKGIEENTAKLMTQVMEDELPPSMQRSLMVLSGPSFASEFSAGKPTAVCLAG
                     VDEPLVQRFQRALMTPAFRVYADTDVIGVQLGGALKNVMALAAGVIDGLDLGLNARAA
                     LITRGLAEIIRLGTAMGADPRTFYGLSGIGDLVLTCTGTLSRNHSVGVRLGKGERLET
                     ILAGMQAVAEGVRTSRAALTLARHYQVDMPIIHEINAVLYDNKPCRKAVGDLMEREAK
                     PEKGWA"
     gene            1265..2020
                     /gene="larB"
                     /locus_tag="VN039_09840"
     CDS             1265..2020
                     /gene="larB"
                     /locus_tag="VN039_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWV46300.1"
                     /translation="MNPEGLERLLRQIRHGQVTVEQALQRLRSLPFEDLGFASLDHHR
                     SLRQGFPEVILCEGKTTAQVIAIARALIRKQGPFLATRADPSVARAIRRLDRRAQYYP
                     DARIVAIRTSRPKQHGHILVVTAGTADVPVAEEARVTAEVMGSDVERLYDVGVAGIHR
                     LLGKKDRLFDAQVVIVVAGMDGVLPSVVGGLVHCPVIAVPTSRGYGASFGGVAALLTM
                     LNSCAAGVGVMNIDNGFGAACLAHRINMLGVKS"
     gene            complement(2042..2590)
                     /locus_tag="VN039_09845"
     CDS             complement(2042..2590)
                     /locus_tag="VN039_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46301.1"
                     /translation="MKTGSVFSLKIANNRHPRKLPLGLLALCGCLAAFFPADGLSKVK
                     PSRVPRPEPELKIVELNIAPTPYTLGNGPLQFSVTVQLPREVDGSAILEVTSLISSPS
                     KSSIRFLTHRQPVLVPAGNGGMERSKVSVELAWDGQDHRKQLAEVGTYSYEVRTKLLA
                     NGEKGLRTVMVAWPKRGILEVK"
     gene            2771..3988
                     /gene="larC"
                     /locus_tag="VN039_09850"
     CDS             2771..3988
                     /gene="larC"
                     /locus_tag="VN039_09850"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HWV46302.1"
                     /translation="MGRHLHFDCFSGVSGDMILGALVSAGLSWTDLINGLKRLQLSGY
                     RLRKREVHRGALPAIKVDVIVQQGFQRPLTLSRILKILAGSTLPGRVKERCRSVFDRL
                     AEAEGMAHGVDPKDVHFHEVGVVDSFIDVVGGVLGCYLLNATRITSSPINVGAGSIQT
                     SHGILPVPGPAVAVLAKGIPIYATGPRCELATPTGVALLRTLAVEFGPMPTMKSMVVG
                     YGAGDHNPDGWPNALRVFLEEESASEVRQTERMIHIETNLDDLSPQTYEYVMEQLFLV
                     GAVDVALVPVVMKKSRPGVMLSCLAAEDRTDAVLEVLFQETTTLGVRLHEVRRQVLPR
                     RIVSVTTQGGVVRMKVAEVGAGWEKAAPEYEDCKVIAKRTGRPLKTVMEEALRIYRRG
                     FPKNRMVHVRGGA"
     gene            3985..4986
                     /locus_tag="VN039_09855"
     CDS             3985..4986
                     /locus_tag="VN039_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46303.1"
                     /translation="MTALYYVLLFVVSVAVTLGGCAIFTNAIEWLGKRLGISEGAVGS
                     IFAAIGTTLPETSIPIIAIFFGESREEVEVGLGAILGAPFMLSTLVLPLLAVLLLLYA
                     RAGKRTARFHLNYREVLTDLTFFMIGYFIALGCVFVPSRLIHLIAAVILICLYIYYMK
                     LKFAPAGGEEGGELEPLIFNKSAAIPSYVMIGFQALLGLGGLIVGAHLFVMAAESMAG
                     LFAMSPLILALLIAPLATELPEMSNSFLWLYRKKDRLAVANVTGAMVFQGTFPVSLGL
                     IGTEWSIAPSALTTMILAVQSVGLCLLQILIGGQWRPWLLAAGALFYIGYTVHLYVN"
     gene            4976..6058
                     /gene="pdxA"
                     /locus_tag="VN039_09860"
     CDS             4976..6058
                     /gene="pdxA"
                     /locus_tag="VN039_09860"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWV46304.1"
                     /translation="MSTESRSLRKRSSSSRLPLLGITMGDPSGIGPEVIAKALSGARL
                     HNVCRSIVIGSLPVMERTIKALKLKLKVFRVHGHETTAPRRGTVAVLDPLDTPLRAFK
                     PGTAAAETGAASVVFIKKAVELAQIGCIEGMVTAPINKEAIYMAGCRYPGHTELLADL
                     THANESGMMIVGGPLRIMFVTTHVAIRDLSLLLTQAKIEKAIRLAQSALTTLFGIKRP
                     RIGVAALNPHAGENGLFGDEEARVILPAARATQREGILASDPLPADTLFGKAAKGSFD
                     GVVALYHDQGLIPLKLVAFGTCVNLTVGLPIIRTSVDHGTAFDIVGKGVADPGSLIEA
                     VKLAAKIARNRTAATPAKKGRAGHVA"
     gene            6048..6368
                     /locus_tag="VN039_09865"
     CDS             6048..6368
                     /locus_tag="VN039_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46305.1"
                     /translation="MSPDMSKGLTVVRQQIDELESLKKQTLQDLNTIAGTERIAQWKV
                     HTVALITDAVGHLEGQAFARIQPGPSFTNDLVEEFADLVECYRSPLIILAKRLAAQAP
                     PPER"
     gene            6372..7193
                     /gene="rsmA"
                     /locus_tag="VN039_09870"
     CDS             6372..7193
                     /gene="rsmA"
                     /locus_tag="VN039_09870"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWV46306.1"
                     /translation="MDSPLPPAAIKRLGQNFLIDPNIVRKIVALAELTPTDTVLEIGP
                     GRGILTEALCRAVGHVTAIEVDPRLHIYLTERRPQFSNLALVLGDAMTYPIEHLPIDT
                     IVVANLPYYVSTPLLFRLLDQRNRFPRMVLMLQNEVADRLMAKPGGSDYGVLSVMAQY
                     AAEITKAFRVSAQCFRPRPEVDSAVVLLKTRHQRALNREEEPKFSALVKAAFAHRRKT
                     LVNSLKDQGYEQKSVAAASESLNLSPSTRAEVLSIEQFIELTRRIYGTGFSNLRR"
BASE COUNT         1515 a         2049 c         2100 g         1604 t
ORIGIN      
        1 aaacccctgc ggcatatggt tgtttcatcg gagcctccga attgcccata tccgtgcccg
       61 actcccatcc tatggcttca acgcatggtc acgaagctat ttctgaattc ctgacgacga
      121 aggaagaacg agtgacctgc cgttcacagc atagcccatc ttcattgaca ggcctttgac
      181 gccttcctat aatccgccgt tctgaaatgg acgaatgatg atttcgccat gaggctgaat
      241 cgcttatgcc gaccacgatc aacaagatcg gcgttatcgg agccggagcc tggggcacgg
      301 ccctggcaaa acatctggcc gagaagggtc tggaggtttg cctctgggcc tatgaacagg
      361 atgtcgtcaa cgccatcaac tcctcacacg aaaactcggt cttcctcaaa aacgtcccgc
      421 ttcctccagg tttgacggcc acttcctcgc tcgttgaagc ggtaaagagc cgagacgggg
      481 ttctgtttgc cgttccttcg cacctgactc gatccgtgtt gcatcaattg gcaccctgca
      541 tccccggctc cctgccgttc gtgtgtgcaa ccaaaggcat agaggaaaac acggccaaac
      601 tgatgaccca ggtcatggag gacgagttgc cgccgtccat gcagcgttct ctcatggtgc
      661 tttccggacc aagtttcgcg tcggaattca gcgccggcaa accgacggcc gtctgtctcg
      721 ccggcgtcga tgaaccattg gtgcagcggt ttcaacgtgc gttgatgacg cctgcatttc
      781 gggtgtacgc ggacacagac gtcatcggcg ttcaactcgg cggtgcctta aagaatgtta
      841 tggcgctggc agccggtgtg atcgacggtc tggacctcgg gctcaatgcc cgcgccgcgc
      901 tcataaccag aggtctggct gaaatcatcc ggctgggcac ggcaatggga gccgatcctc
      961 gcacgttcta tggactttcc ggtatcggcg atctggtact gacctgtacg ggcacactga
     1021 gccgaaacca ttcggtaggc gttcggctcg gcaagggaga acggttggag acgatattag
     1081 ccggaatgca ggctgtggca gaaggcgttc ggacgagtcg tgcggcactc acgttggccc
     1141 gtcactatca ggtcgatatg ccgatcatac acgagatcaa tgccgtcctg tacgataata
     1201 aaccctgtcg aaaagcggtc ggtgatttga tggagcggga agcaaaacca gagaaaggat
     1261 gggcatgaat ccggaaggac tcgagcggct gctacggcag atccgtcatg gacaagttac
     1321 ggtggaacag gcactgcagc ggctgcggtc attgcctttt gaggatctgg gctttgcttc
     1381 gctcgatcat catcgatcgc tgcgacaagg gttccccgag gtgatcttgt gtgaaggaaa
     1441 gaccacggcg caggtcatcg ccatcgctcg ggcactcatc agaaagcagg gaccgtttct
     1501 ggcgactcgt gccgacccat cggtcgcgcg tgctattcgc cgtctggatc ggcgagcaca
     1561 gtactatccc gatgcccgca ttgtggcgat tcgcacctcc aggccgaagc aacatggaca
     1621 tatactcgtg gttaccgcag gaactgcgga tgtgcctgtg gcggaagaag cccgtgtgac
     1681 cgcagaagtg atgggaagtg acgtcgaaag gctgtacgat gtcggtgtcg cgggcatcca
     1741 tcggttgctc ggaaagaaag atcggctgtt tgatgcgcag gtcgtgatcg tcgtcgccgg
     1801 tatggacggc gtcctgccga gtgtggtggg aggtttagtc cactgtcctg tgattgccgt
     1861 accgaccagc cgagggtatg gtgcgagttt cggcggagtt gctgcgctgc tgacgatgct
     1921 caattcctgc gcagcgggtg tgggagtgat gaacatcgac aacggattcg gcgcagcctg
     1981 cctcgcgcat cggatcaata tgttgggggt aaagagttag cgtgagagag acaatggaac
     2041 gctattttac ttccagtatg ccccgcttcg gccaagcgac catcacggtg cgcagccctt
     2101 tctcaccgtt tgccaacaat tttgttcgca cttcatacga ataggtgccc acctcggcaa
     2161 gttgcttgcg gtgatcctgc ccatcccaag ccagttcgac ggacaccttc gatcgttcca
     2221 tcccgccatt tcccgcaggg acgagaacag gctgccgatg cgtgagaaat cggatcgagc
     2281 tcttcgaagg agaactaatc agcgatgtca cctccaaaat cgcagatcca tccacttctc
     2341 tcggaagttg taccgtgacg gaaaactgaa gaggcccgtt tcccagggta tacggtgtcg
     2401 gcgcaatgtt gagttcgaca attttgagct caggctcggg cctgggaacg cgggagggct
     2461 tcacctttga aagcccgtcc gctgggaaga atgcggccag acaaccacac agggcgagca
     2521 accccagcgg aagtttccga ggatgtcggt tgtttgcaat tttcaaagaa aacaccgaac
     2581 ctgttttcat aggtgagagt ggcgcattca ccgcgatgca cggtccgtgc gactcaactg
     2641 catcgttagg gggataacat agcatccccg gggtgtcaac gaaccgtcga taatgaaccg
     2701 acgattgccg ttcggcaata cgtttgggta agatgtccgg tcattttacg tggagcgagt
     2761 gaggaattcg gtgggtcggc atttacactt cgattgtttc tccggtgtca gtggagacat
     2821 gatcctgggc gcgctggtga gcgccgggct ctcgtggaca gacttgatta acggactcaa
     2881 acgtctacag ctcagcggtt accgactgcg gaagcgtgag gtgcatcgtg gcgcgctccc
     2941 ggcgataaaa gtcgacgtga tcgtgcaaca ggggtttcaa cggccgctga ctctctctcg
     3001 tatcctaaag atccttgccg gcagcacgct gcctggccgg gtcaaggagc gttgtcggtc
     3061 cgtctttgat cgattggctg aggcagaagg gatggcgcat ggcgtcgatc ccaaagatgt
     3121 tcactttcac gaagtgggcg tcgtagattc gtttattgat gtcgtcggag gcgtgctggg
     3181 ttgctatctc ttgaacgcca cccgcatcac ctcgtctccc atcaatgtgg gggccggttc
     3241 cattcagacc tcacacggga tcttaccggt tccaggaccc gctgtcgcgg tgctggcgaa
     3301 gggaatcccg atctacgcca ctggcccacg ctgcgagctc gcaactccaa ccggggttgc
     3361 gctgcttcgg acattggcgg tggaattcgg acctatgccg accatgaaaa gtatggtggt
     3421 aggctatggg gccggcgacc acaatccgga cgggtggccc aatgccttgc gcgtgtttct
     3481 ggaggaagag tccgcctcgg aggtgcgtca gaccgagcgg atgatccaca ttgaaacgaa
     3541 tctcgacgac ctcagtccac agacctatga atacgtcatg gaacagctct ttctggtcgg
     3601 tgctgttgat gtcgcgctgg ttcctgtcgt catgaagaaa agccgaccag gagtcatgct
     3661 gagctgtctg gccgctgaag atcggacgga tgccgtgctc gaggtcctct tccaggaaac
     3721 gaccacgctc ggagtacgtc ttcatgaggt acgccggcag gtccttcccc gacgaatcgt
     3781 ctctgtcacg acccaaggcg gagtcgttcg catgaaagtc gccgaggtcg gggccggatg
     3841 ggaaaaagcc gcccccgaat acgaggactg caaggtcatc gcaaaacgaa ccggtcgtcc
     3901 cctcaagacg gttatggagg aagcattgcg gatctatcga cgaggattcc cgaagaacag
     3961 aatggtacac gtgcgaggcg gggcgtgacc gctctgtact acgttctcct ctttgtcgta
     4021 tccgttgcgg tcacgctagg tggttgcgcg atctttacca atgccattga atggctgggc
     4081 aaacggctgg gtatctccga gggtgccgta ggcagcatct ttgccgcaat cggcacgacc
     4141 ctgccggaaa cctcaatccc gatcattgcg attttcttcg gcgaaagccg ggaagaggtt
     4201 gaggtcggct tgggcgccat cctaggcgcg ccgttcatgc tcagcacgct ggtgttgccc
     4261 ctattggccg ttcttctgtt gctttatgca cgagccggca agcggactgc acgatttcat
     4321 ctcaactatc gggaagtcct gacagacctc acgtttttta tgatcggata ttttatcgcc
     4381 ttgggttgcg tcttcgtgcc atccaggctc atccacctca tcgcggccgt catattgatc
     4441 tgtttgtata tctactacat gaagctcaaa tttgcaccgg ccggcggtga agaaggaggt
     4501 gagttggagc cgctgatttt caacaagtcc gccgccatac cgtcgtatgt catgatcgga
     4561 ttccaagccc tcttgggttt aggcggattg atagtaggcg cccatttgtt tgtcatggct
     4621 gcagaatcga tggccggttt attcgccatg tcgccgttga ttttggcgct gctcatcgca
     4681 cccctcgcca ccgagctgcc ggagatgtcc aacagctttc tctggctcta tcgcaagaaa
     4741 gatcggctcg ccgtagccaa tgtcacagga gccatggtgt ttcaaggcac cttcccggtc
     4801 tctctggggt tgatcggaac tgaatggtcg attgcaccat cggcattgac caccatgatc
     4861 ttggccgtgc agtcggtggg actctgcctc ctgcagattc tgatcggagg tcagtggcgg
     4921 ccgtggctac tcgccgccgg ggctctgttc tatatcggct ataccgtgca tctctatgtc
     4981 aactgaatcg cgatcacttc gcaaacgttc ctcctcttcc cgactcccct tgctgggaat
     5041 cacgatggga gacccgtccg gcatcggccc ggaagtgatc gccaaggctc tgtccggagc
     5101 tcgcttgcac aacgtttgcc gatcgatcgt catcggatcg cttcccgtca tggagcgaac
     5161 catcaaggca ctgaagctca aactgaaagt attcagagtt cacggtcatg aaacgacggc
     5221 gccgcgcaga ggaacggtgg ccgtactgga tccactggat acaccgctgc gagcgttcaa
     5281 gcccggtacg gcagcagcag agaccggtgc cgcttcagtg gtctttatca aaaaggctgt
     5341 tgaattggct cagatcggct gtatcgaggg aatggtgacg gcacccatca acaaagaagc
     5401 catctatatg gccggctgcc gatacccggg ccataccgag ttgttggccg atctcactca
     5461 cgccaacgaa tcaggcatga tgatcgtggg cggcccgtta cgtatcatgt tcgtcacgac
     5521 acacgtcgcg atcagagacc tctcgttgct gctgactcaa gcaaagattg aaaaagcgat
     5581 ccgcttggct caatcggcgt tgacgacgct gttcggcatc aagcgtccca ggatcggagt
     5641 ggctgctctc aatccccatg ccggtgagaa tggattgttc ggcgatgagg aagcccgcgt
     5701 gattcttcca gcggcccgtg cgacgcagcg ggaaggtatc ctggcaagtg atcctctacc
     5761 ggcggatacc ctattcggaa aagccgcgaa agggtcattt gacggtgtcg tcgcactgta
     5821 tcacgatcag ggcctcattc ccctgaagct tgtcgctttc ggcacgtgcg tcaatctcac
     5881 cgtgggcttg cccatcatcc gcacttcagt agaccatgga acggcttttg atatcgtcgg
     5941 aaagggcgtc gccgatcccg gaagtctgat cgaggccgtc aagctggcgg ccaagatcgc
     6001 tcggaataga acggcggcaa ctccagccaa gaaggggcgg gccggacatg tcgcctgata
     6061 tgtcgaaggg gcttaccgtg gtccggcagc agattgacga attggaatct ctcaagaaac
     6121 agacccttca agatctgaac acgatcgccg ggaccgaacg aatagcacaa tggaaggtgc
     6181 acaccgtggc gcttatcacc gatgccgtcg gccatctgga aggacaggca ttcgctcgta
     6241 tccaaccggg gccgtcgttc accaacgacc tggtcgaaga gtttgcggat ctcgtcgaat
     6301 gttatcgctc gccgttgata atactagcca agcgattggc ggcccaggct cccccacctg
     6361 agaggtgacg ggtggactcc cctctccccc ctgcagcgat caaacggctc ggacagaatt
     6421 ttctcatcga tcccaacatt gtccgtaaaa tcgtcgcgct ggctgagctg accccgaccg
     6481 ataccgtctt ggaaatcggc cctggacgcg gcatcctcac tgaagcgctt tgtcgtgccg
     6541 tcggccacgt gacggcaatc gaggtcgatc cacgactcca catttacctg actgaacgac
     6601 ggccccagtt ttcgaatctc gcgctcgtcc tcggcgatgc gatgacctat ccgatcgaac
     6661 accttccgat cgataccatc gtcgtcgcga atcttcccta ttatgtgtcc acacctctcc
     6721 tcttccggct tctcgatcag cgaaaccgtt ttccccgcat ggtgctgatg ctgcaaaatg
     6781 aggtcgcaga tcgcttgatg gcaaaaccgg gcggctccga ctacggagtg ctttccgtca
     6841 tggcacagta tgcggcggag atcacgaaag cgttccgagt ctcggcgcag tgttttcgac
     6901 caagaccgga agtcgactct gctgtggttc tcctaaagac caggcatcag agagcactga
     6961 accgggagga agagccaaaa ttttccgcac tcgtgaaggc cgccttcgcg caccgccgta
     7021 aaacgctggt caattcgctg aaagatcagg ggtacgagca gaagtcggtc gcagcagcat
     7081 cggagtccct caatctctct ccctccaccc gtgcggaagt cctttccatc gagcagttca
     7141 tcgaattgac tcgccgcata tatgggacag gcttctcgaa cctccgccga taacaactgc
     7201 cgctcgggat gcttggaaag ccggcgttgg gcgtgctcgg cacgctacgc gggtcgcatg
     7261 tggatttt
//
LOCUS       DATMTI010000619         3715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_574722, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000619 DATMTI010000000
VERSION     DATMTI010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574722"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VN039_09875"
     CDS             <1..1484
                     /locus_tag="VN039_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWV46307.1"
                     /translation="CLMQLSIFWRLVMTSLVIITVMGGVNVYALFQLRQLTAMSTQMA
                     SYHYPAAESAKRLLGSLYAQLNSEKKYLATNDHTFLTNLTEEVDEFQHSLQLLRGQES
                     SPQGLKLLKETDELLKEQLKLFRNELQTAKGKLRQVTPEYENRRDVLMDRMSSSIQGY
                     IDLHEARVSVGVTESRASAAQAEAVTEQLVLVALVFGLGLAGIASYTILRPLRELQGH
                     IKQIGQGNFGASLQIKAPAELRELVDTVNWMGRKLQEIDNMKTEFLAHVSHELRTPMA
                     SIQEGTHLLLDEIPGPLVQEQRTTLRIMADSSKRLIHLISTILDLSKMEAGMMEYRIV
                     PVDLQRIGEISINKVRLLADSKHVQLVLESVGERAWVKADASRLEQVLDNLLSNALKF
                     SPEGGVVKVQMKPDPQAGVLEVAVSDTGPGIAPEDLPHVFERFVQGRMKAKHTAGSGL
                     GLALAKKVVEAHGGRIWIESEKGKGATVRFILRLAKPETVGQA"
     gene            1700..2266
                     /locus_tag="VN039_09880"
     CDS             1700..2266
                     /locus_tag="VN039_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46308.1"
                     /translation="MRGLLGLYESREIAEKYFEKVLTVAPKSHLAASSKAWLELLQQP
                     TVPGHKSWPEAVFRAPVLAETNTSLASTADRLVRDLLDRELLIQQLRASKGDDSEVVG
                     ALQRELADRDHKIESLLSKKDSVKAPADPASIQSLQKQLAERDRKIEELSTQLEALKR
                     IDQEMREKVRPMRPPLPTVPVPGPETTP"
     gene            2287..3705
                     /locus_tag="VN039_09885"
     CDS             2287..3705
                     /locus_tag="VN039_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV46309.1"
                     /translation="MEQENILVVDDDEGLLHLLKMRLSAMGFSVTPCTTGHDAVGEAK
                     KTMFNLAITDLRLRGEDGLDVTEELLRSHPGLPVIILTAHGSIPNAVEAMQRGAFGYL
                     TKPFDDKELKATIEKALAQQRMSREIQRLKSLVKELYGLENVVARSPAMQRLFQQIAQ
                     VADSDATILLFGETGTGKEVMARVIHTNSRRGKGPFVALNCAAIPETLFESELFGHVK
                     GAFTSALGAKRGLFQMANGGTLFLDEIGEMPLSMQVKLLRAVQEREIREVGSDISVKV
                     DVRIIAATNKDLSEAVKNGTFRNDLYYRISVVPLFIPPLRDRRDDIPLLAQHFLKLSV
                     KRANKEVKGFTPAALHRLMVNPWPGNVRELENVVEKAVVMSRQDMLTPDLLPAVSVSP
                     DSPLKPLTEAKEEFERTYLKNVLQLTSGNISRAAQFAGRYRADFYKMLRKYGLHPSTT
                     KGRSDSEMEELESEANLTEAER"
BASE COUNT          896 a          939 c         1106 g          774 t
ORIGIN      
        1 actgcctcat gcaactttca atcttttggc gattggtgat gacctctctc gtcattatta
       61 cagtcatggg aggagtcaat gtttacgcac tctttcagct taggcaattg acggcgatga
      121 gcacccagat ggcgtcttac cattatccgg ccgctgaatc cgcgaagagg ctattgggtt
      181 cgctctatgc ccaattgaac agtgaaaaga aataccttgc gaccaacgat cacacctttc
      241 tgacgaatct gactgaagag gtcgatgagt ttcagcacag tctccaactg ttacggggcc
      301 aggaaagctc ccctcagggg ctgaaactgc tcaaagaaac ggacgaattg ctcaaggagc
      361 agctgaagct gtttcggaat gaattgcaga ccgcgaaggg aaagctccgg caggttactc
      421 cagagtatga aaaccgccgg gatgtcctga tggatcggat gtcctcttcg attcagggct
      481 atatcgatct gcacgaggct cgagtgagtg tcggggtgac ggaatcccga gccagcgccg
      541 cccaagccga ggccgtcaca gaacaactcg tcttggtggc gttggtgttc ggcttggggc
      601 ttgccggcat tgccagctat acgattttac gtccgctccg agagctgcaa gggcacatca
      661 aacagatcgg gcaaggaaat ttcggggcat cccttcagat caaagctcct gcggagttac
      721 gcgagctcgt cgataccgtg aattggatgg ggagaaagct tcaggaaatc gacaatatga
      781 aaacggagtt cctcgcgcat gtgtctcacg aattgcgcac cccgatggcc tccattcaag
      841 aaggcacaca tctcctcctt gatgagattc cgggtccgct cgtacaggaa caacgaacga
      901 cccttcggat tatggccgac agcagtaagc ggctgatcca tctgatctcc acgattctgg
      961 atttatcaaa aatggaagcc gggatgatgg agtaccgcat agttccggtg gatcttcaac
     1021 ggatcgggga aatctccatc aacaaggttc gtctgttggc agactccaaa cacgtgcaac
     1081 ttgtgctcga aagtgtcggc gagcgggcgt gggtcaaggc ggacgcttcc cgtctggaac
     1141 aggtgctcga caatttactg tcaaacgctc tgaaattcag tcctgaggga ggtgtggtga
     1201 aggttcagat gaaacccgac ccacaagccg gagtccttga ggtggctgtt tcggatacgg
     1261 gacccgggat cgctccagag gatctgccgc atgtgttcga acgttttgtt caaggccgca
     1321 tgaaggcgaa gcatacggcg gggagtggat tgggtttggc attggcgaag aaggttgtcg
     1381 aagctcacgg cggaagaatc tggatcgaaa gtgagaaggg caagggagcg actgtacggt
     1441 ttatcctacg gttggcaaaa ccagaaacgg tagggcaagc gtgacaagaa aagcactgtc
     1501 tctgacaaga gcccccatca tcatcagttc catcttatta ttgataagct gtgcagcttg
     1561 gataacgcca ccacccggct ctcgtcccta tttttccgca gagcctcgag aagtcaaagt
     1621 ttttcaatcg cttgcaaaga agcaagagag tgtagcctca aaatgtggcg aatcgagctc
     1681 ctgcgaccac atctacttta tgcgcggatt gctcggactc tatgaaagtc gcgaaattgc
     1741 ggagaaatat tttgaaaagg ttttaacggt tgctcccaaa agccacctgg ccgcgtcaag
     1801 caaagcctgg cttgaattgc tccaacaacc aaccgttcct gggcacaaat catggccgga
     1861 agccgtattt cgagccccgg tccttgcaga gaccaatacg tcattggcat cgacggctga
     1921 tcgtctggtg cgggacttgc ttgaccggga gttgctcatc caacaacttc gtgcatccaa
     1981 gggcgacgat tccgaggtgg ttggggcctt gcagcgcgag ctggcggatc gagatcacaa
     2041 gatcgagagc ctgttgtcga agaaagactc ggtcaaggcg ccggccgatc cggcgtcgat
     2101 tcaaagtctg cagaaacagt tggccgagcg ggacagaaag attgaggagc tttccacgca
     2161 actcgaggcg ttgaaacgaa tcgatcagga gatgcgggaa aaggtgcgac cgatgcgtcc
     2221 gccgttgcct accgttccgg ttccgggccc cgagacgaca ccctaacgta tgagagaagg
     2281 gattatatgg agcaagaaaa cattctcgta gtggacgacg atgaggggct gttgcatttg
     2341 ttgaaaatgc ggttgtccgc gatgggtttt tcggtcaccc cttgtacgac ggggcatgat
     2401 gcggtcggtg aggcgaagaa gacgatgttc aacctggcga tcaccgacct acgtcttcgc
     2461 ggagaagatg ggttggacgt gaccgaagag ctcctcagga gtcatccggg acttccggtc
     2521 atcatcctga cggcccatgg gagtattccc aatgccgtgg aggcgatgca acgaggcgcg
     2581 ttcgggtacc tcacgaagcc gtttgatgat aaggagctga aagccacgat cgagaaagcg
     2641 ctcgcgcaac agcggatgag ccgagagatt cagcggctca agtcgctggt caaggaactg
     2701 tacggactcg aaaatgtcgt ggcgcggagt ccggcgatgc aacggctctt ccagcaaatc
     2761 gcgcaggtgg ccgattcgga tgcgaccatc ttgctgtttg gagaaaccgg aaccggcaaa
     2821 gaggtgatgg cgcgtgttat tcatacgaac agccggcgcg ggaaggggcc tttcgtggca
     2881 ctcaactgcg ccgccatacc tgaaacgctg ttcgagagcg agttgttcgg acatgtaaag
     2941 ggcgcgttca cgagcgctct cggcgcgaag cgcggattgt ttcaaatggc caacggcggc
     3001 acgctgttcc tcgacgagat cggtgaaatg ccgctgtcca tgcaggtcaa attattgcgc
     3061 gccgtacagg agcgggagat tcgagaagtt gggtcggaca tctcagtcaa agtcgatgtc
     3121 cgcatcatcg cagcgaccaa taaagacctc agcgaggcgg tgaagaacgg gacgttccgc
     3181 aacgatctgt actatagaat ctcagtggtg cctctgttca ttccgccgtt gcgggaccgc
     3241 cgggacgata tcccgctatt ggcccaacac ttcctcaagc tcagcgtgaa acgagccaac
     3301 aaggaggtga agggattcac gccggccgca ctccatcggc tcatggtcaa tccctggccg
     3361 ggtaatgtgc gcgagcttga gaacgtggtc gaaaaggccg tggtgatgtc gcggcaagat
     3421 atgctcacgc cggatttgtt gccggcggtc agtgtgtcgc ccgattcgcc gctcaagccg
     3481 ctgacggagg ccaaagaaga attcgagcgg acctatttga aaaacgtcct ccaattgacg
     3541 agcggaaata tttcccgtgc cgcgcagttt gccggccggt accgggccga cttctataag
     3601 atgctgagaa agtacggcct gcatccgtcg accacgaagg gaaggtcgga ttccgagatg
     3661 gaagagctgg aaagcgaggc gaatttgacc gaggcggagc gatagatctc gagtc
//
LOCUS       DATMTI010000620         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_236832, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000620 DATMTI010000000
VERSION     DATMTI010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236832"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..879
                     /locus_tag="VN039_09890"
     CDS             31..879
                     /locus_tag="VN039_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46310.1"
                     /translation="MTAKVLTNLESIGDLVGALSGIDSALDDQMYMEGLIKSAHGRAA
                     TAFDLAAAATAKTGRMSHVYEYGTIGVTRGMPKFADPASPKARLYVHTLVGQGGEQSI
                     GYTFRPARQPNPKPTPESTGVDSKYLSRLSGRQYYFYNKAYVMETGRVVEIKPRNGNF
                     LFVPFYGEASRDPLNNRGYMMWDSRRLGPLSARPGRSTKGEFTQFWMQWWATSGSKMM
                     YAHMEEQVNIDVGIAMAEAQKRAQTEAMKPVQDTNIVGAISTAKALFNKIFKASTSKR
                     MGTKTL"
     gene            876..1427
                     /locus_tag="VN039_09895"
     CDS             876..1427
                     /locus_tag="VN039_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46311.1"
                     /translation="MTDPTLGPDLLLRQYAWALLKANDPAVWDESNYGGVVPIVPLSD
                     EPELSDFDGPHIVYGYTGDTTGDHWMESSGTMTFAVYEDNFRRLGKTLNILHAAFERA
                     DDSAKDVNHYLDTVSLDGSTHPFRNTIRFGCIEIGFVEGGIPETTEGGRQSGLLNIRF
                     DFFVDYDVNVGRYDPTTGSYLGF"
     gene            1582..2205
                     /locus_tag="VN039_09900"
     CDS             1582..2205
                     /locus_tag="VN039_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46312.1"
                     /translation="MATKKNIIIGAAQVYLGPSWDTTNGANPAPAAVDTGGGVSYVTT
                     MNGSGTWTNVGYTQDGIEVATDPTWDDVEVDQLLDAAKIFKSGMSLTVATTFAESTLE
                     NLLIAWGQATSTLNSFASYRQLDIDGGSLGSAPLERGLIAVGNAPEKANTNNYGERTY
                     HAYRVLSVESVSHTLARAEATVIPVTFRALPGDNGKYGYVRDRMSLA"
BASE COUNT          667 a          573 c          680 g          596 t
ORIGIN      
        1 gtttatactt ctagaaaggg ctgaggtgtc gtgacagcca aagttctgac caatctagaa
       61 tccattggag acctggttgg tgcgctcagt ggaattgaca gtgccctaga tgaccagatg
      121 tacatggagg gactaatcaa gtcggctcac ggacgcgcag cgacagcctt tgacctcgca
      181 gcagcggcga cggcgaagac tgggagaatg agtcacgtct acgagtatgg aacaatcgga
      241 gtcacgcgag gaatgcccaa gttcgcagac ccggccagcc cgaaagccag actgtatgtt
      301 catacactgg ttggccaggg tggagaacag agtattggat ataccttccg tcctgctagg
      361 cagccgaacc caaagccgac cccagaatcc acaggagttg actcaaagta tttgtcgaga
      421 ttgagtggta ggcagtatta tttctacaac aaggcatatg tcatggagac cggccgggta
      481 gtggaaataa agcccaggaa tggaaatttc ctattcgttc cattctatgg agaggcttct
      541 cgtgacccac tcaataacag gggctacatg atgtgggatt ctcgtcgcct tggaccgctc
      601 agcgcaagac caggacgctc taccaaggga gaattcacac agttctggat gcaatggtgg
      661 gcaacatctg gaagcaagat gatgtatgca catatggaag aacaggtgaa tattgacgtc
      721 ggcattgcaa tggcagaggc tcagaagcgt gcccagacag aggcaatgaa gcctgtccaa
      781 gacacaaata tcgttggagc cataagcact gccaaggcat tattcaacaa gattttcaag
      841 gcgtctacat ctaagagaat ggggactaag acattatgac cgacccgacg ctaggacccg
      901 acctactact tcgtcaatat gcatgggcac tcttgaaagc gaacgacccg gcggtatggg
      961 atgagagcaa ctatggtgga gtagttccca tagtaccatt gtcagacgag ccagagttga
     1021 gtgacttcga tggcccacac atcgtctatg gatacacagg agatacgact ggtgaccatt
     1081 ggatggagtc ttctggaaca atgacattcg cggtttatga agacaatttc cgcaggctag
     1141 gcaagacgct gaatatcttg catgcagcat tcgagagagc agacgatagc gccaaagacg
     1201 tgaaccatta tcttgatact gtgtcattgg atggttcgac gcatcctttc aggaacacaa
     1261 tcaggttcgg atgtatcgag ataggcttcg ttgagggcgg tatcccggag acgacagagg
     1321 gcggtaggca gtcaggtcta ttaaacataa ggttcgactt cttcgtagat tatgacgtca
     1381 atgtcggcag atatgacccg actactggct catacctagg tttttgagtt ttcatttgct
     1441 tttgtttttt gtcgggatac aagtgtgaag agcgatagag tggcagacta cagactccaa
     1501 tttgcttgat aatatggatg caggtatagt taactacgag aagcgctata aatacttttg
     1561 acatggaggt gaatatcact aatggctaca aagaagaaca tcatcatcgg tgctgcacag
     1621 gtctacctgg gtccgtcctg ggacaccact aacggtgcaa accctgctcc tgctgccgtt
     1681 gacacgggcg gtggggtaag ttacgtcacc acgatgaacg gctctggtac ctggacaaat
     1741 gtcggttaca cacaggatgg catcgaggtc gctactgacc ctacttggga cgacgttgag
     1801 gtcgaccagt tgctcgacgc tgcaaagatt ttcaagtctg gaatgagcct aaccgtggca
     1861 accacgttcg ctgagtcaac tctagagaac ttgctcattg cttggggtca ggcaacatcc
     1921 acgcttaact cctttgcatc ctacaggcag ttggacattg acggcggctc actaggctct
     1981 gctcccttgg agcgcggtct gattgcggtc ggcaatgcac ctgaaaaggc gaacaccaac
     2041 aattatggtg agcgtacata tcacgcatac cgagtcctgt ctgttgaatc tgtttcccac
     2101 acgctcgcgc gtgctgaggc aacagtcatc cccgtcacct tccgtgcgct tccgggcgac
     2161 aatggtaagt acggatacgt ccgtgaccgc atgtcactag cgtgagatag gtctttagac
     2221 ttgtataaga gggcatcctt ttggatgccc tctttgcatg tttatagaaa tttgcgtctg
     2281 atggagttgt cacatataat ggtatcaaca caacaggagg aataaagtgg caacacgagt
     2341 atatacgaca gaggatatca ccttacaaga cggtaccgat gtcacattga agccgttggc
     2401 gattggtcgt ctgcgtcgtt tcatggacgc ttggagagaa ttcgagaatg tagatggcga
     2461 cgatgagggc ttcgatgtct ttgttaattg tgccggaatt agtctcgaag agaatt
//
LOCUS       DATMTI010000621         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_533092, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000621 DATMTI010000000
VERSION     DATMTI010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533092"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..651)
                     /locus_tag="VN039_09905"
     CDS             complement(250..651)
                     /locus_tag="VN039_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46313.1"
                     /translation="MSFESDVQNIGSIDNTNSATTTTYASSVTSGFTFSTSQTISAEA
                     SFEASAEVVKVGFKVGLSISFTEQWSSSQTTEFSFSVPGGKRAFTYQGYLLSRILRYD
                     PSTDNYTYLSEVARFVTNILSPSEVPLVDSQ"
     gene            complement(708..1010)
                     /locus_tag="VN039_09910"
     CDS             complement(708..1010)
                     /locus_tag="VN039_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWV46314.1"
                     /translation="MPMKNPPHPGEFIRTEIIVPVGLSVTAAAAALQVSRPALSSLLN
                     GKADLSGDMAIRIEKAFGVNMDTLMRMQSAYDIAKTRKRAKGIRVRRVQRAVGLHP"
     gene            complement(1028..1423)
                     /locus_tag="VN039_09915"
     CDS             complement(1028..1423)
                     /locus_tag="VN039_09915"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWV46315.1"
                     /translation="MSADSNTPQPVSIEVFAPLFVGHIDNRYVTGYSRAVHIRNFIHK
                     GLKRLYEEGSTRGVPPDTVDKLRKMLAYLDAIESSEELRALTAWKAHMLTGDRKGIWS
                     LSVTRNRRLTFRIDTAERELCDVNLEDYH"
BASE COUNT          368 a          444 c          413 g          393 t
ORIGIN      
        1 aacgtcctca ggaacctatg acttccctgg ctcgccgttt acaatcgaca agatcgcaac
       61 ctatattgtg acgaatcttc aacagaaaac gacctacttc ttcgcagttt ctgcctatga
      121 cctcgccgga aacgaaagtc ccctctcgaa tgaagtcagc aagagcatct tttgacggct
      181 gctgaaccgt cctcattgtt gcgcaagatg agattcctcc ctcagcgcat tctatgcggg
      241 ctcgacgcat tattgcgaat cgaccagcgg tacctcacta gggctgagga tgttggtgac
      301 gaatctggcc acttctgaca aataggtata gttgtctgtg gatgggtcat aacgaagaat
      361 cctggacagc aagtatcctt ggtaagtaaa tgcccttttg cctccgggca ccgagaagct
      421 gaactcggta gtctggctgg aagaccactg ttccgtaaag gagatactca agccgacctt
      481 gaatcccacc ttgaccactt cggccgaggc ttcgaaggat gcctcggcgc tgatggtctg
      541 cgatgtgctg aaggtgaaac cgctggtcac gctcgatgca taggtcgtgg tcgtagcgga
      601 attggtattg tctatgctgc cgatattctg cacatcgctt tcaaagctca attcctctaa
      661 tcgtcactga ccagttacgc agcacccgtc attgatggtg aagagcatca aggatgcagg
      721 ccaaccgcgc gctggacacg ccggacacgg ataccctttg cccgcttgcg ggtcttggca
      781 atgtcgtagg cagattgcat ccgcatgagc gtgtccatat ttacgccgaa agctttctcg
      841 atgcggatcg ccatgtcgcc ggacaagtcg gccttgccgt tgagcaaact ggacagggcg
      901 gggcgggata cctgaagcgc tgccgcagcc gccgtgacgg atagtcctac gggaacaatg
      961 atctccgtcc gaataaactc tccaggatgg ggcggattct tcatgggcat agccggtact
     1021 cctccttcta atgatagtcc tctaaattca cgtcgcaaag ttcgcgttcg gcggtgtcaa
     1081 tacggaacgt cagacgtcgg tttcgggtaa cgctgaggct ccatatgcct ttgcgatcac
     1141 cggttaacat atgcgccttc caagcagtca acgccctcaa ttcttcggag ctttcgatgg
     1201 catcaaggta cgcgagcatc ttcctcagct tgtccaccgt atcaggcggc acgccccttg
     1261 tgctgccttc ttcatagagc cgcttaagac ccttatgaat aaagtttcgt atgtgcaccg
     1321 cacgactgta gcccgtaacg taacggttgt caatgtgacc aacgaacagg ggagcaaaca
     1381 cctcaataga gacaggttga ggcgtattgg aatctgcgga catcttaagg taggaacaga
     1441 attaaggggc cagaccccct ttctttggtt tgaatgaacg gttccccgct tccgcttatg
     1501 actcttccag ttgaatcgtc tccacatgga ccagggcgac tcctttgtcg acaaatccaa
     1561 gctgttcggc tgctccttcg ctcaagtcga tgatcctctt ccccgaatca ggattgtg
//
LOCUS       DATMTI010000622         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_363316, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000622 DATMTI010000000
VERSION     DATMTI010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363316"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..337)
                     /locus_tag="VN039_09920"
     CDS             complement(104..337)
                     /locus_tag="VN039_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46316.1"
                     /translation="MDNNKASAAILVILELRPGFWEYTHKHENGAYAAWIQVQRLGYY
                     SVSELAYDPVRDVYAFDVLTSGTPSGRRPWSVT"
     gene            818..1204
                     /locus_tag="VN039_09925"
     CDS             818..1204
                     /locus_tag="VN039_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46317.1"
                     /translation="MAEIKFTAFVNGAVQDSRSDDNFVIKTAEAHRRKVRGGKDDEYE
                     TYGRTFRDVFLSVDADKELADWFAGTVFEEGMRIDVIGQEVTRPREYQGKTFYDLVVY
                     ATAIAVHEAQAPAKGKAPARRTTARR"
     gene            1270..2070
                     /locus_tag="VN039_09930"
     CDS             1270..2070
                     /locus_tag="VN039_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46318.1"
                     /translation="MATLGELREQVARAQRNATAKIGRLKNEGIIVAGSDKDVRVTRD
                     RLNRYNRGQLLSQLAKVNTFNARSTKFVAGIEGTPISLAVASAHAQAERLITQRANRQ
                     YAGVRETPLPSGNTIGFVEEVVRGDKRGSMKGNHRPLEPVHRPASNFTSEKAMKAVTR
                     KLNRQLSTTRHRGEIKKQAERVEEALKAFGNDKLSQAFKQLTPYQTDILLNYTHSQSS
                     FFDKYLEMMLALRGKEGRIINENAEDESFEWLNWALGLPKQAPTNPKK"
     gene            2174..3748
                     /locus_tag="VN039_09935"
     CDS             2174..3748
                     /locus_tag="VN039_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HWV46319.1"
                     /translation="MEWGTTIESFFDRCMEEPARYDFFNLAFDGKFIIDWLMKNGYVH
                     VEKSKHEGGPKPGEFTTMISNMGKWYSFTVKWLNGITTEFRDAAKKFPANMGVAGVAK
                     AYGLDVSKGELDYAKPRPEGYQPDSDELDYLDRDVRIVAKALSVQFAAGLGKLTASSD
                     ALNEFYDVVGGKEAFRKTFPLLSDDMDAQIRKAYRGGWTYLSPRFKQCKVSSGTVYDV
                     NSLYPSVMYNELIPYDLPVFKPGKVSTTAERPIAIFSVTFTAKLKADHVACIQIKGTS
                     MFGATEYLTEVNEPTQLTVTSVDWALWNEQYDITVYSWDGGYLFKARAGMFCQYIDKW
                     MQVKAESTGGQREIAKGQLNNLYGKFATNPDITGKIPVLDGELVRYVTGRAETKEPVY
                     TAAGVFITAYARAKTIRVAQLNYDRFIYADTDSGHFTGTAPILGAEIHPTKLGAWKHE
                     YYFSEALFVRPKVYLERMVLSGQMHTAFAGLPREVASSLTFADIVHGKELRGKLHPKS
                     VEGGVILQDVVYTVKL"
     gene            3795..4148
                     /locus_tag="VN039_09940"
     CDS             3795..4148
                     /locus_tag="VN039_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46320.1"
                     /translation="MDIQKSEYVAPPKAKSEATAPKPKPEIVAAIQPYVAEGIDTLFD
                     VTTETDNWERDKRFIQTAANSLGVAARIPKDGLVHFTDEAGNAMTTITFRVRPMRVLK
                     SKDETVEPDSDVAGY"
BASE COUNT         1055 a         1254 c         1286 g          872 t
ORIGIN      
        1 actccgtgtt gccagcgggt gatttcctgc ccgtcacgtg tcacgataac ccggatggga
       61 ccatcgaaca ggatacggtg aacaacgccg accttctcga ttttcaggtc accgaccacg
      121 gacgacgccc ggatggtgtc cccgacgtca agacgtcgaa ggcgtagacg tcgcgcacgg
      181 ggtcgtaggc cagttcgctg acgctgtagt agccaaggcg ctgaacctgg atccatgcgg
      241 cgtacgcacc gttttcgtgc ttgtgggtgt actcccagaa accggggcga agttcgagga
      301 tgacgaggat tgcggctgat gctttgttgt tgtccatggt tctattgtaa acagaccaac
      361 cgtgaatgtc aagcccatgc acagggattt tctgaacagg ccgcataccg gtttatgtac
      421 ggaactagtt cgttagcggt taaatgaacg ccgtttatta acaagtgcta actacccgtg
      481 gaattgggcg tgtcgtgatt tgcggttgtg gctatgtgtg ctgtagagtc gtagacgagg
      541 cgctagagta ccactgcctc gggtgagttg aacacttgta tagactaccc atgcacaaac
      601 gcgtaggggt tggtggttgc cgaccgttat tcggcctagc ctacgccata tcgcgttcct
      661 gtccctccct gggcgcgcga cggtccggcc ctccgtgaat tgggcatcgc tcatggctcg
      721 accatcccag catggcatcg gccctttgag agcccgcggg agaaacgcgt aattgcgtca
      781 tccgcggcgg gcacaatcca agatgaaaga tgaaacaatg gcagaaatca agttcactgc
      841 gttcgtgaac ggtgcggtgc aggactcccg ctcggatgac aactttgtca tcaagacggc
      901 agaggcgcac cgtcgcaagg ttcgcggtgg taaggatgac gagtacgaga cgtacgggcg
      961 tacgttccgt gacgtgttcc tgtccgtgga cgccgacaag gaacttgcgg attggttcgc
     1021 aggtaccgtg ttcgaagagg gaatgcggat cgacgtcatc ggtcaggagg tgactcgccc
     1081 gcgcgagtac caggggaaga cgttctacga tctggtcgtg tacgctaccg ccatcgccgt
     1141 gcacgaggca caggcacccg cgaagggtaa ggctcccgcc cgccgcacga ccgcgcgtcg
     1201 ctgagtgtta cactgaggcc ccgtccgcta cggtgggcgg ggcctcacca tcgctactga
     1261 gggggatacg tggcaacact gggcgaattg cgcgaacagg tcgcacgggc acagcgcaac
     1321 gcaacggcca aaatcggacg actcaaaaac gaaggcatca tcgtcgcggg atctgacaaa
     1381 gacgtcaggg taacccgcga cagactcaac cgctacaacc gtgggcagtt gctcagccaa
     1441 ctagccaagg tgaacacgtt caacgcacga tcaaccaagt ttgtagccgg tatcgaaggc
     1501 actcccatta gcctcgccgt tgcgagcgca cacgcccaag ctgaacggct catcacccaa
     1561 cgtgccaacc gtcaatatgc gggcgtccgt gagactcccc taccgtcggg taacactatc
     1621 gggtttgtgg aagaggtggt gcgcggcgat aaacgcggtt ccatgaaagg caaccaccgc
     1681 cccctggagc ctgtgcaccg cccggcgtcg aacttcacgt ccgagaaggc tatgaaagcc
     1741 gttacccgga aactgaatcg gcagttgtcc acgacacgtc accggggtga aatcaagaaa
     1801 caggctgaac gtgtcgaaga ggcgttgaaa gcgtttggca atgataaact gagtcaagcg
     1861 ttcaaacagt tgacaccgta tcaaactgat attctgctca actacacgca cagtcaatcg
     1921 tcattctttg acaaatatct cgaaatgatg cttgccctcc ggggtaaaga gggaagaatc
     1981 attaacgaaa atgccgaaga cgaatcgttc gaatggctta attgggctct cggtctcccg
     2041 aagcaagcac cgaccaatcc gaaaaagtaa agcgcggccc atctattcgg cggacttcga
     2101 aaccattaca acagcgccca cgcgcgtatg gttgtggggc attctcgacg ttgccaatcc
     2161 cgaccatggg gacatggaat ggggaaccac cattgaatcg ttcttcgatc gatgcatgga
     2221 agaacctgcc cgctacgact ttttcaacct tgcgtttgat ggaaagttca tcattgattg
     2281 gctgatgaaa aacggttacg tgcatgtcga gaagtcgaag cacgaaggcg gtccgaaacc
     2341 gggcgagttc accaccatga ttagcaacat gggcaaatgg tattcgttca ccgtcaaatg
     2401 gctgaacggc atcaccacag agttccggga cgccgcgaag aaatttcccg ccaatatggg
     2461 cgtggcaggt gtcgccaaag cctacggact tgacgtgtcc aagggcgaac tggactatgc
     2521 gaaaccacgc ccggaaggtt atcagccgga cagcgacgaa ctggactatc tggatcggga
     2581 cgtgcgaatc gtcgccaaag cgctgtctgt gcagttcgcc gcgggactcg ggaaacttac
     2641 cgcctcatcc gacgccctga acgagttcta cgatgtcgta ggcgggaaag aggcgttccg
     2701 gaagacattc cccctactat ccgacgacat ggatgcacag atccgcaaag cctaccgtgg
     2761 cggatggacc tatctgagtc cgcgtttcaa acaatgcaag gtctcatccg ggaccgtcta
     2821 tgacgtcaac agcctgtacc cctcggtcat gtacaacgag ttgatcccct acgatctgcc
     2881 cgtgttcaag cccggcaagg tgtccaccac agcggaacga cctatcgcca ttttctctgt
     2941 cacgttcacg gcgaaactga aagccgatca tgttgcgtgt attcagatca agggaacgtc
     3001 catgttcggg gcgacagaat atctgaccga agtcaacgaa cctacacaac tcaccgtgac
     3061 ctccgtggac tgggcgttgt ggaatgaaca gtacgacatc accgtctact cgtgggacgg
     3121 tggttatctg ttcaaggcac gtgctggcat gttctgtcag tacattgata aatggatgca
     3181 agtcaaagcg gaatcgacgg gcggtcaacg tgaaatcgca aagggacagc ttaacaattt
     3241 gtatggcaag ttcgccacaa acccggatat cacggggaag atacccgtat tggatggcga
     3301 attggttaga tacgtcactg ggcgtgccga gaccaaagaa ccggtataca ccgcggcggg
     3361 cgtgttcatc accgcctatg ctcgcgccaa gacaatccgg gttgcacaac tcaactacga
     3421 caggttcatc tacgccgaca cggatagcgg gcacttcacc ggaacggccc cgatcctcgg
     3481 cgcggaaatc cacccaacca aactgggcgc atggaaacat gaatactact tctccgaagc
     3541 gctcttcgtc cggcccaagg tatacctaga gcgcatggtg ttgtccgggc agatgcacac
     3601 ggcgttcgcg ggtctgcccc gcgaggtggc atcatcgctc acgttcgctg acatcgtgca
     3661 cggtaaagag ttgcggggta agttgcaccc gaagagtgtg gagggtggtg tgatcctgca
     3721 agacgttgtc tatacggtga aactctgata gtctgaaacc atcaatggtt cacggaaaca
     3781 gaaaggatga accaatggac attcagaaaa gcgagtacgt cgccccgccc aaggctaaga
     3841 gcgaggccac ggcccccaag ccgaagcccg aaattgtcgc cgccatccag ccttacgtcg
     3901 ccgagggtat cgacaccctg ttcgacgtga ccacggagac cgacaactgg gagcgtgaca
     3961 agcgtttcat tcagaccgcc gcgaacagcc tcggcgtcgc cgcccgcatc ccgaaggatg
     4021 gtcttgtgca cttcaccgac gaggcgggga acgcgatgac gaccatcacg ttccgggtgc
     4081 gtccgatgcg cgtcctcaag tcgaaggacg agaccgtcga gccggattcg gacgtcgcgg
     4141 gctactgaaa tcgtgacgct cggaactcgc cgttcggact cgccacctgt tgccccgttg
     4201 agtcggaagg tgtgctagca tgggggtcgc atcccgtcga accgtgagcc gggttgtcca
     4261 cacggcacca tacggtgcca ccaactagaa agggggtccc tcgaattggg accccctttc
     4321 gcttcgattg aaagggccag acatggctaa ggttgacgat ctggtcaatt ccgtaggcgt
     4381 gtccgaagag gacgggatca ctcccctcta tccggacacg ttccgtgacg acatcctcgg
     4441 cgcatccgcc gaagatgcgg cactcgc
//
LOCUS       DATMTI010000623         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_65268, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000623 DATMTI010000000
VERSION     DATMTI010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65268"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..543
                     /locus_tag="VN039_09945"
     CDS             166..543
                     /locus_tag="VN039_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWV46321.1"
                     /translation="MNRVRIVTIALGIGAASGGPALDLSPATQILMENGSPYYVPATA
                     TVTSGTPVRWDNPTPTHHTVTHNDCVKEAHPCLFNSGTVAPGGHFTVPGLPAGRYSYH
                     CGIHPIMRGQLVVTDNPSTPSQL"
     gene            654..2108
                     /locus_tag="VN039_09950"
     CDS             654..2108
                     /locus_tag="VN039_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV46322.1"
                     /translation="MTGEVLALLAWHIPDLVAYHHHPDEWWFAPLDDNLPLIRLNRIG
                     LDMLKEMNGHTTVGMLIEQYGTKVCGPDGQPGSWHLERWATPNYSLCYFGAAAPGGHR
                     HKAKWDTLLQQVREGWSGREGFEGEEHLEEFHRHELAQSEEDDRHFDLIETTVSHLFR
                     EPSEALSGLTYGRLLMRQLRRLGWFNPKPKVLLEIGGGLGYLAQELGKDLLPFEKQRM
                     TYLSLDITQPFLKLQVTRAKIGGWNVSGTRANAESLPFADHSVDLVIDNENMADMTPV
                     QLGKQELTCGVGETPQHQEALDWIRRLRLPIENDPPESVIFNLGPIRFAAELWRVLKP
                     GGRAFLTEFGIEDGWPAPVKLPRHTEYEVQYSHLRHAIRWLGFQERYLSLPQFLELKP
                     DTKVLCTGATYTIQRFCQSISRPFAVRAYTEKELQQALGDMLPKLQGLHYHDLADPAW
                     FGLTDFKVLLLEKPGGAPRAQFTENKGYRWYSQK"
BASE COUNT          499 a          676 c          579 g          464 t
ORIGIN      
        1 ctgggcgtcc gatgaagcga tgaggcatgg agaaaggttg acagagcatt cccaagcgat
       61 ccagctgaat cccatgctat tatgatgggg ccatagaaac gagtctcact cttccgcttt
      121 tcccggctct tatcaacagt ccgtcttgta aggagtgcca ccgacgtgaa ccgagtacgc
      181 atcgtgacga ttgccttagg aatcggagcc gcctcgggtg ggccggccct ggacctctct
      241 ccagccacac agattctcat ggagaacgga tcgccgtatt acgttcctgc caccgccacc
      301 gtaacaagcg gcacgcccgt tcgttgggat aatccgacgc ctacccacca tactgtcact
      361 cacaatgact gcgtgaagga agcacatcca tgcttgttca attccgggac ggtggcacct
      421 ggaggtcact tcactgtgcc tggcttgccg gccggccgtt actcctatca ttgcggtatc
      481 catcccatca tgcgaggtca actggtcgtg acagacaatc cctccacacc ctctcaattg
      541 taaacacgcc gatcatcttg cgcgattgct gattgctcct gtaggctgct ggaactcatc
      601 accaaaaacc gctcgccatg aaacccgctg tcgctgtcca tgaaccccga agcttgaccg
      661 gagaggtcct ggccctcctt gcttggcaca ttcccgacct ggtcgcctat catcaccacc
      721 cggacgaatg gtggtttgcc ccgcttgatg acaacctacc gctcatccgg ctgaaccgca
      781 tcggcctgga tatgctcaag gaaatgaacg gccatacgac tgtcggtatg ctcattgaac
      841 aatatggcac gaaggtctgt ggaccggatg gacaaccggg atcctggcac cttgaacgat
      901 gggctacccc aaactattcc ctctgctatt tcggagcggc cgcgccagga ggccatcgac
      961 acaaagccaa gtgggatact cttctgcagc aggtccgcga aggctggtcg ggtcgggaag
     1021 ggttcgaagg cgaggagcat ctggaagaat tccaccgtca cgaactcgcc caaagcgaag
     1081 aagatgaccg tcattttgat ctgatcgaaa ccaccgtctc acatctcttc cgtgaaccga
     1141 gcgaagcact gagcggcttg acctacggcc ggctcctcat gcggcaactg cgccgactcg
     1201 gctggttcaa tcccaagccc aaggtcctct tggaaatcgg cggggggctt ggctacctcg
     1261 cgcaggagct tggcaaggat ctgctgccct tcgaaaagca gagaatgaca tacctatcgc
     1321 tcgacatcac gcagccgttc ctcaaactcc aagtcactcg agccaaaatc ggtggttgga
     1381 atgtttccgg caccagggcc aatgccgaat ccctcccgtt tgccgatcac tccgtcgatc
     1441 tcgtgatcga caacgagaac atggcggaca tgacacccgt gcaactgggc aaacaagagc
     1501 tgacgtgcgg ggtgggagag actccgcaac accaagaagc actggattgg atcagacggc
     1561 ttcgcctccc gattgaaaac gacccgcctg aatcggtgat ctttaactta ggaccgattc
     1621 gcttcgccgc cgagttgtgg cgggtgctca aaccgggtgg ccgagcgttt ctcaccgagt
     1681 tcggcattga ggacggatgg cctgcgcccg taaagttgcc gagacacacg gagtacgagg
     1741 tccaatacag ccacctgcgg cacgccatac gttggctcgg ttttcaggag cggtatcttt
     1801 ctcttccgca atttctggaa ctcaagccgg acacgaaagt cctctgcacc ggcgcaacct
     1861 ataccatcca aagattctgt cagtcgatca gccgcccctt cgccgtgcgc gcctataccg
     1921 agaaggaatt gcaacaagcc ttaggcgaca tgctccccaa acttcaaggc ctccactatc
     1981 acgacctcgc tgatcccgct tggttcggtc taaccgactt caaggtactg ttgctggaaa
     2041 aacccggcgg ggcgccgagg gcgcaattca ccgagaacaa aggctatcgg tggtattcgc
     2101 agaagtaaaa cgtcgttgcc gatcgttagc aatcggcttc ggttcaaggc atacgataag
     2161 tctcacggca ttccgcttag gccgctttat ctaccgtgct catttttagt cgttccca
//
LOCUS       DATMTI010000624         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_23716, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000624 DATMTI010000000
VERSION     DATMTI010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23716"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VN039_09955"
     CDS             <1..336
                     /locus_tag="VN039_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46323.1"
                     /translation="YTQGAWERFLEVLAENPDDAEAIHWLLRAGTAQNRWQELVEQLS
                     AYVVRNPGDLAVRFALASVLLRAQQLDDAQREYGQLRMLAPNYDGLIELGQALANKEA
                     VLAYEASNS"
     gene            346..1527
                     /locus_tag="VN039_09960"
     CDS             346..1527
                     /locus_tag="VN039_09960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWV46324.1"
                     /translation="MASLIVQLARLGDLLQTLPAITSLTARYAGERFDLLCAATLTDI
                     VSLFPGTARAVPWEADQWQQWALGWTNSPDSTMTAAQDHIHQLAAQTYDTVYNLNQHP
                     RAILAAHLLGRRVVGPGSAGPLSENLPPWGTYLRKVARDRSANHVHLADAFCGLCGVM
                     PPGRVPRITSPVSDLPGSLEKIGDPRYHWVAVIIGAGDLARCLPPAIWARWITALLNA
                     HTAIRVVLIGNSGEIGRSRAIQETLSPLLWGRVWDATGHTNLLQLALLLSRCRWTIGS
                     DTGSLHLAAAVGCRTLGLYFARARVHETGPYGDQHWVWQASTFAPDTWPIDESLDFIV
                     AQTSAPQLTECTGWQLWEGHVDAWGMSYSRPGQSNVSVDDRMTVWKRCMSMDDRENVL
                     L"
     gene            1524..>2525
                     /locus_tag="VN039_09965"
     CDS             1524..>2525
                     /locus_tag="VN039_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3880 domain-containing protein"
                     /protein_id="HWV46325.1"
                     /translation="MSVFEDNLREIVKQSPELAAALRAAQGGRLEIRPTKSGHPTGFA
                     QGRAIHSTYDPVKESTSWAAAQIMSCREGDVVLVFGIGLLYHVEALCKVLPPSIRLVV
                     AVPDLVELHDACAVRAWGDWVRRVTWVWGVPHSMAKRMRELGSSVRLMTYVPAARLHS
                     DAYAAIEESLRRSIAHAAGGQLRIAVVGPIYGGSLPVTRSVVAALESLGHHVDWIDHS
                     AHATSYQALARLKDVRHRQVMQNKFAEFLSQLTLVRIAERPPDLVLAMAQAPMTLPVL
                     EHIRKKHFLTAMWFVENYRHLTYWQQMAAGFDFWFVIQKGGCPDAFRHAGAKEIHYLP
                     "
BASE COUNT          498 a          703 c          748 g          576 t
ORIGIN      
        1 tacacacaag gagcctggga acggtttctt gaggtgctgg ccgaaaaccc cgatgatgcc
       61 gaagcgatcc attggctgct ccgtgcaggc acggcgcaaa accgctggca agagttggtt
      121 gagcaactga gcgcctatgt cgtgagaaat ccaggtgatc tggcagtgcg ttttgcgttg
      181 gctagtgtcc tgctacgtgc ccagcaactg gacgatgctc aacgagaata cgggcaactg
      241 cgtatgctcg cgccgaatta tgacggtctt atcgaactcg gccaagcatt ggcgaacaaa
      301 gaagcggtcc tggcatacga agcatcaaat tcgtagtgtg catccatggc atcactgatc
      361 gtgcaactgg cgaggcttgg ggacctgctc cagacacttc cggccatcac ttcgttgacg
      421 gcccgatatg ccggcgagcg atttgacctg ctctgcgccg caacgttgac cgatatcgtg
      481 tcgttattcc ccggaacggc aagagccgtt ccatgggagg cggatcagtg gcaacagtgg
      541 gcgttaggtt ggaccaattc tcccgattca acgatgacgg cagcacaaga ccacatacat
      601 caactggccg cgcagaccta cgataccgtg tacaacctca atcagcatcc ccgtgccatt
      661 ttggcggcgc atttgctcgg caggcgggtt gtcggaccgg gctccgccgg accgttatct
      721 gagaatcttc cgccttgggg tacttatctc aggaaggtcg ccagagaccg gagtgcgaat
      781 catgtccacc tcgccgatgc cttctgcggt ttgtgtggcg tcatgccccc cggtagagtg
      841 cctcgaatta cctctcctgt ttccgatctt ccaggaagtc tagagaaaat cggcgatcct
      901 cggtatcact gggtggctgt gatcatagga gccggagacc ttgcccgctg cctgccccct
      961 gcgatttggg cacgatggat cactgcactt ctgaacgccc acactgccat ccgtgtggtt
     1021 ctcatcggaa acagtggtga gatcggacgc agccgcgcca ttcaagagac cctttcgccg
     1081 ttattgtggg ggcgtgtttg ggatgcaacg ggtcatacga atctgctgca attggctctg
     1141 ctactctccc gctgccgttg gacgatcggc agcgatacgg gttctctgca tcttgcggcc
     1201 gctgtcgggt gtcgaacgct tggtctatat tttgcgcgtg cacgtgttca tgaaaccggt
     1261 ccgtatggag accagcattg ggtctggcag gcgtcgacat ttgctccgga cacctggcca
     1321 atcgacgaaa gccttgattt catcgtcgct cagacgagtg ccccacaatt gacggagtgt
     1381 accggctggc agttatggga ggggcatgtt gatgcgtggg gaatgagtta ctcgcgccct
     1441 ggccaatcga atgtctccgt cgacgatcga atgaccgtct ggaaacgctg catgtcgatg
     1501 gacgatcggg agaatgtcct gctatgagtg tatttgaaga taatcttcgt gaaattgtca
     1561 aacaatcacc ggaactcgcg gcggcgctgc gtgctgcgca gggtgggcga ctcgaaattc
     1621 gaccgacgaa atcgggacac cccacaggtt tcgcgcaggg ccgcgccatc cacagcacct
     1681 acgatccagt caaagaatcg acctcttggg cagcagctca gatcatgtct tgccgtgagg
     1741 gagacgtcgt cctcgtgttc gggatcggtc tgctctatca cgtggaggcc ctctgcaaag
     1801 tgcttcctcc atcaattcgc ctcgtggtgg ctgttcccga cttggtcgaa ttgcatgacg
     1861 cctgtgccgt gagggcgtgg ggagattggg tcaggcgtgt gacctgggtg tggggagtgc
     1921 ctcattccat ggccaagcgt atgagggaac tcggatcttc cgtgagactc atgacctatg
     1981 tgccggctgc ccgattacat tccgatgcgt atgccgcgat cgaggagagt ttgcgacgct
     2041 ctattgccca tgctgctggc ggtcagcttc gaattgccgt tgtgggtccg atttatggag
     2101 gctctcttcc ggtcaccaga tctgttgtgg cggctctgga atcactcggc catcacgtcg
     2161 attggatcga ccatagcgcg catgctacca gttaccaggc gctggcccgt ttgaaggatg
     2221 tccgccaccg acaggtgatg caaaacaagt tcgccgaatt tctcagccag ttgactctcg
     2281 tgcggatcgc ggaacgtccg ccggacctgg tcttggccat ggcccaggcg ccgatgaccc
     2341 tgccggttct cgaacatatt cggaagaagc attttctgac cgccatgtgg ttcgtcgaaa
     2401 actaccgcca cctgacgtac tggcaacaga tggcggcggg attcgacttc tggttcgtga
     2461 ttcaaaaagg gggatgcccg gatgcgttcc gtcatgccgg ggccaaggag atccattatc
     2521 tcccc
//
LOCUS       DATMTI010000625         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_744919, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000625 DATMTI010000000
VERSION     DATMTI010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744919"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /gene="secF"
                     /locus_tag="VN039_09970"
     CDS             <1..930
                     /gene="secF"
                     /locus_tag="VN039_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWV46326.1"
                     /translation="KGARMLEILGKTNIDFMGKRKFSFLFSGIMVLLGLIALVQIARG
                     AANLGIDFAGGTAVQLKFEQTVRIDEARKALETNGLSDAELQEFGQDNKLLIRVKAST
                     TIEEKTAERVVGIFAKEFPNNKFVVDSTTEIGPTIGKKLQEDALVAVVISFAGIILYI
                     AARFELRFGVAAALATFHDVLAVVGAFYILDKEITLLIVTALLTLAGYSLTDTVVVFD
                     RIRENLKLRRRESEEATINSAINQVLSRTIVTSLTVVLVLVPLTMAGGEVLHDFSLAL
                     LWGVIFGTYSSVFVASPLLLLWPGAPGRLLKRG"
     gene            1048..>3877
                     /locus_tag="VN039_09975"
     CDS             1048..>3877
                     /locus_tag="VN039_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV46327.1"
                     /translation="MALWSRSHSLQQKIIAAIVMVGLLPLTLLLALIYVEERRALRES
                     TGANFKEVAVEAARRIEIHITQGMNEAQQLATTPFLRTAVSEANRTYEGKDAQSISEI
                     IKDWQQRWGQRDKRSEFPHFVNRIVTNYLIRWHEIRKSDYVGILVTDGQGALVVSSIP
                     QVEYSYAKTAWWQAVIKGGSQQPYVSEIAFDPSFGTHVVIVAAPILDDQRRAVIGAVT
                     ILLRRDTLFQSIAEGSFGATGHAMLFASDGGGVVICPILAPEAHSIDAELLGTMGAPK
                     PGWAVAADDSHGNKRALIGFAPVRIAGQLAAGSLGGKRWITLVRQDAAETFAPLGELM
                     TKMLLFGSVVLIGLGGIGVIVARRIARPIQLLHDGVQQIGSGRLEQRVELKTGDEIEG
                     LAQAFNHMASNLQRSFGQLEQRMTEVRQLEEKYRDLIEHAPEMICQLDRGGRLVHVNK
                     TGLDKLGYTPDEMLGMKLWDCVRSGQESRVLHFLEQLVSHGQSSMETVLVAKDGRLMD
                     VEVHGTALFDQERGRLIHSRAFVRDVTERHRLEQEIQRYTVGLEQAVSERTHQLTVSQ
                     ARYKALFDFVADSVFMVSATGAVVAVNEREERVLGYAESKIVGKNFLDIVPDVHHQAF
                     TGWLCDVSTEQRQVATQEMTVYHADRDEIPVEMDLIRVGGTEPLLVMVQLRDITDRKK
                     LERQLQSYREDLELKVRERTREIEETKQYLENLLENANDVIYTLDLNQQFTYVNGKVN
                     AWGYRKDDLIGRPYLSLLSRRHRGRRLKSTLDIGAKQVYEVEVVTRLGEARAVMVSVS
                     PLQGADGEILGVLGIARDMTETKKLERQIRNAEKLASIGQLAAGVAHEINNPLGGILN
                     CLYNLRKGPLSPARQEEYWASMEHGVRRVQKIVRQLLDFSQQHEPAFSPSDINRIVDQ
                     VLGLTTHLFVPNQIRLETFPGNGLPNVMV"
BASE COUNT          859 a         1009 c         1158 g          851 t
ORIGIN      
        1 aagggagcgc gcatgttaga gattctcggg aagaccaaca ttgactttat gggcaagcgc
       61 aagttctcct ttctcttttc agggatcatg gtcttgctcg ggctcatcgc actggtccag
      121 atcgcacgag gtgcggctaa tctgggcatc gactttgccg ggggcacggc tgtccaactc
      181 aagttcgagc agacggtccg gattgatgag gcgcgaaagg ctctcgaaac caacggtctg
      241 agtgatgcag agctgcagga attcggacaa gataacaagc tcctcatccg ggtgaaagct
      301 tccacgacga ttgaagaaaa gaccgcggaa cgcgtggtgg gaatcttcgc caaagagttc
      361 ccgaacaaca agttcgtggt ggactcaacg accgagatcg ggccgaccat cggtaagaag
      421 cttcaggaag acgccctcgt ggccgtcgtc atttcatttg ccggtatcat tttgtacatc
      481 gcggcgcgat tcgagcttcg gttcggcgtc gcggctgcat tggcgacgtt tcacgacgtc
      541 ttggccgtgg tcggggcttt ctatatcctg gacaaagaaa ttacgctcct gatcgtgacg
      601 gcgctcttga cgctggccgg ctattcctta accgacaccg tcgtcgtgtt cgaccggatc
      661 agggaaaatc tgaagttgcg gcgtcgagaa agcgaggaag caacgatcaa ttccgccatc
      721 aatcaggtgt tgagccgcac gatcgtcacc agtttgaccg ttgtgctcgt cctggtccca
      781 ttgaccatgg caggaggaga agttctgcac gatttctcac ttgcgttgct ctggggtgtg
      841 attttcggca cctattcatc ggtattcgtt gccagccctc tcttattgtt gtggcccgga
      901 gcgccgggtc gactcttgaa acgcggctga gcgaatggtg gaatagagcg ggcgtcgctc
      961 gtgaagcgtt gttcgtcgtt cgtttccgaa gatcattcag aacgcgtcac ccttcacagg
     1021 tttccgcatc attagcggag cacccttatg gccctctgga gccgatctca cagcctccag
     1081 cagaaaatca tcgcagcgat cgtgatggtc ggtctcctgc cgcttacact cctgctcgcc
     1141 ctcatctatg tcgaagagcg acgcgccttg cgagagtcga cgggggcgaa tttcaaagaa
     1201 gtggccgtcg aagccgcccg tcggatcgaa atacacatta ctcaaggtat gaacgaagcc
     1261 caacaactcg cgacgacacc cttccttcgc accgccgtct cggaggccaa ccgaacgtac
     1321 gagggcaaag acgctcaaag tatttccgaa atcatcaagg actggcaaca gcggtggggg
     1381 cagcgcgata agcgaagcga atttccgcac ttcgtcaacc ggatcgtgac caactacttg
     1441 attcgatggc acgaaattcg aaaatcagac tatgtcggca ttttggtcac cgatggacag
     1501 ggcgccttgg tcgtcagttc aattcctcaa gtggagtatt cctatgccaa gaccgcctgg
     1561 tggcaggctg tgataaaagg cggcagccag cagccttatg tgagtgaaat cgccttcgac
     1621 ccatcgtttg ggactcacgt ggtcatcgtg gctgctccga tcctggatga tcaacgtcgg
     1681 gctgtgatcg gggctgtaac gatcctgctt cggcgtgaca cattgttcca gtccatcgct
     1741 gagggctcct tcggcgcgac aggccatgcc atgttgttcg cctcggacgg gggtggtgtg
     1801 gtgatttgcc caatccttgc tcccgaggcc cactcgatcg atgccgaatt gctcggtacg
     1861 atgggtgctc cgaaaccagg atgggcagtg gctgccgacg attcgcacgg aaacaagcga
     1921 gccctgatcg ggttcgcacc ggtgcggatt gccggtcaac tggcggcggg cagtctcggt
     1981 ggaaaacggt ggattaccct tgtgcgtcag gatgcagcgg aaacgttcgc tccgctgggt
     2041 gagctgatga ccaagatgct gttgttcggc tcagtggtgc tgatcggcct tggggggatc
     2101 ggagtcatcg ttgcccgccg gatcgcgcgg ccgattcagc tgttgcacga cggagtgcag
     2161 cagattggga gcggtcgctt ggagcagcgg gtggagttga agaccggcga tgaaatcgaa
     2221 ggacttgccc aggccttcaa tcacatggcg tccaatctgc aacgttcgtt cgggcagctg
     2281 gaacagcgaa tgacagaggt tcggcagttg gaagaaaaat atcgagatct gatcgaacat
     2341 gctccggaaa tgatctgtca actcgatcga ggcggtcgac tggtgcatgt caataagacc
     2401 ggcctcgaca aactcggata tacccccgac gaaatgttag gcatgaagct atgggattgt
     2461 gttcggagcg ggcaggaatc gcgcgtgttg cactttctgg aacagcttgt ttcacacggg
     2521 caaagctcga tggaaacggt gctggtggcc aaggatggcc ggttgatgga tgtggaggtt
     2581 catgggaccg ctctattcga tcaagagcga ggcaggctga ttcactcgcg tgccttcgtt
     2641 cgagatgtga ctgaacggca tcggttggag caggagatac agcggtacac cgttggattg
     2701 gaacaggcgg tgtcggagcg tacacatcag ctgacagtct ctcaagcccg ctacaaggcg
     2761 ttgttcgact tcgtggccga ttccgtcttt atggtgagtg caacgggggc cgtcgttgcc
     2821 gtcaatgaac gggaagaacg ggtgctcggc tatgccgagt cgaagatagt cggaaaaaac
     2881 tttctcgaca tcgtaccgga cgtgcaccac caggctttta cgggctggtt gtgcgatgtc
     2941 agtacagagc aacggcaggt cgccactcag gaaatgaccg tgtaccacgc tgatcgcgat
     3001 gagattcccg ttgaaatgga cctgatccgc gtgggtggga ccgaaccatt gctggtgatg
     3061 gtgcagcttc gcgatattac cgaccggaag aagctcgaac gccaactgca atcataccgg
     3121 gaagatctcg agctgaaagt ccgggagcgc accagagaaa tcgaagagac caagcagtac
     3181 ttggaaaatt tgctcgagaa cgccaacgac gtcatttata cactcgatct gaatcaacag
     3241 ttcacctatg tcaacggcaa ggtcaatgcc tgggggtatc gcaaagatga tttgatcggc
     3301 cggccgtatc tctcgctcct ttcacggcgt catcgaggac ggcgtctcaa gagcacgtta
     3361 gatatcggag ccaagcaagt gtacgaggtc gaagttgtga ctcggctggg tgaagcgcgt
     3421 gccgtcatgg tgagtgtctc tccccttcaa ggggccgacg gggagatcct tggagtgctt
     3481 ggcatcgcgc gtgacatgac ggagaccaag aagttggagc gacagatacg taatgcggaa
     3541 aaactggctt ccatcggcca gctcgcggca ggagtggccc atgaaatcaa caatcctctc
     3601 gggggaatcc tgaattgtct ctacaacctt cgaaaagggc ccctctctcc ggcacgtcaa
     3661 gaggaatact gggcctccat ggaacatggc gtccgacgtg ttcaaaagat cgttcggcaa
     3721 ttgctcgatt tttcgcaaca gcatgaaccg gcgttcagcc cgtccgatat caatcgaatc
     3781 gtcgatcagg tactggggtt gaccacccat ctctttgtcc ccaatcagat tcgcttggaa
     3841 accttcccgg gtaatggatt gcccaacgtg atggttg
//
LOCUS       DATMTI010000626         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_363513, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000626 DATMTI010000000
VERSION     DATMTI010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363513"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..310)
                     /locus_tag="VN039_09980"
     tRNA            complement(235..310)
                     /locus_tag="VN039_09980"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(275..277),aa:Ala,seq:tgc)
     gene            complement(325..401)
                     /locus_tag="VN039_09985"
     tRNA            complement(325..401)
                     /locus_tag="VN039_09985"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(365..367),aa:Ile,seq:gat)
     gene            complement(600..2081)
                     /locus_tag="VN039_09990"
     rRNA            complement(600..2081)
                     /locus_tag="VN039_09990"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          513 a          739 c          612 g          567 t
ORIGIN      
        1 cgccggctcg ccacagactg gaacgatacg aaccgcgttt cacaacgcgg tttgattttt
       61 gatcggtcag atcaaattaa ccagaattca cgtggctcca ctgctcttcc cggggtgcgg
      121 gtcaaacaag cagaaaagcc atctctttac gatgtcgaaa gatccggcag cgcgccgtcc
      181 gaagacgggc gaactgccaa acttgctctc tgtgtgagac gtatcttggt agattggtgg
      241 agccgatcgg gatcgaaccg acgacctgaa gcttgcaaag ctaccgctct cccaactgag
      301 ctacggcccc gttccatcgg tggatggtgg gcccgggtag actcgaacta ccgaccttac
      361 gcttatcagg cgtacgctct aaccacctga gctacgggcc ctctggatca cgccagggtg
      421 ccggcagctc gccgggctcg aacgctcgcg aacaagtgct gttcgcgacg ctgacgcgtg
      481 aatacgtcgc ttgtgaagaa agagaaacga aggcggcgaa gttccgcaaa gtgacgcctg
      541 acttggcgtc ttttgttcca atgaaagacc gatagccgaa gctgaaggtc aatccttaga
      601 aaggaggtga tccagccgca ggttccccta cggctacctt gttacgactt caccccagtc
      661 gctgacccta ccgtggtcgg ctgcttcctt gcggttagcg caccggcttc gggtagaacc
      721 aactcccatg gtgtgacggg cggtgtgtac aaggcccggg aacgtattca ccgtagcgtg
      781 ctgatctacg attactagcg attccaactt catgcactcg agttgcagag tgcaatccga
      841 actgagacgg cttttaggga ttagcatgtc ctcgcggact agctgccctc tgtcaccgcc
      901 attgtagcac gtgtgtagcc cagcccgtaa gggccatgaa gacttgacgt catccccacc
      961 ttcctccggc ttatcaccgg cagtccctct agagtgccca actaaatgat ggcaactaaa
     1021 ggcgagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga gctgacgaca
     1081 gccatgcagc acctgtgtgc aagtcaccga agtgaaggaa tccatctctg gaaaccgtcc
     1141 tgccatgtca agggctggta aggttcttcg cgttgcttcg aattaaacca catgctccac
     1201 cgcttgtgcg ggcccccgtc aattcctttg agttttaatc ttgcgaccgt actccccagg
     1261 cggagagctt aatgcgttag ctgcgccact gaggggtaaa ccccccaacg gctagctctc
     1321 atagtttacg gcgtggacta ccagggtatc taatcctgtt tgctccccac gctttcgcac
     1381 ctcagcgtca gtaccggacc agtgagccgc cttcgccact ggtgttcttc ctaatatcta
     1441 cgaattccac ctctacacta ggagttccac tcacctcttc cggactcaag atcgccagta
     1501 tcgaaggcag ttctggagtt gagctccagg atttcacccc cgacttaacg atccgcctac
     1561 gtgcgcttta cgcccagtaa atccgaacaa cgctagcccc cttcgtatta ccgcggctgc
     1621 tggcacgaag ttagccgggg cttcttctgt gggtaccgtc attatcttcc ccactgaaag
     1681 agctttacaa ccctaaggcc ttcatcactc acgcggcatg gctggatcag gctttcgccc
     1741 attgtccaat attccccact gctgcctccc gtaggagtct gggccgtgtc tcagtcccag
     1801 tgtggctgat catcctctca gaccagctat ggatcgtcgc cttggtgggc cattacctca
     1861 ccaactagct aatccaacgc gggctcatcc aatcccgata aatctttccc ccgtagggcg
     1921 tatccggtat tagctgaagt ttccctcagt tattccgaag aactgggtag attcccacgc
     1981 gttactcacc cgtctgccac tcaccttgcg gtgcgttcga cttgcatgtg ttaagcctgc
     2041 cgccagcgtt cgttctgagc caggatcaaa ctctcaagtt tgatattctg acttggtcga
     2101 cgcatgcacg tttgcttgac gaggacacac acctgtcact acgaaccgaa gttcgcgtga
     2161 ttggtagtgt acctctgaga aacgtaccgt cgaagtctat tcgtctggtc tcacgaccgg
     2221 accgcaagaa cctcgccgtc cacgtttccc tttcttccat cttcacaatg tcaaagagca
     2281 gaccccttag gccgggtggc caagacgtcg gtggaagcaa ggcttcctaa tttttgggaa
     2341 caagaggttc tatccggttt gcccggcagc acccctgccc tttcaggatt tcgctacgtt
     2401 gcagcgcggc ggcgccgtct gcatcagcga t
//
LOCUS       DATMTI010000627         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_447919, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000627 DATMTI010000000
VERSION     DATMTI010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447919"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1087)
                     /locus_tag="VN039_09995"
     CDS             complement(149..1087)
                     /locus_tag="VN039_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV46328.1"
                     /translation="MSYNILVTGGAGYLGSIMVPDLLQLGHKITVLDSFMYKQASLNH
                     VCHHPNFSVVKGDIRIESVMVPLIKKTDIVIPLAALVGAPMCSQDPVGATTVNHDAII
                     LMLKLLSKQQMVLMPTTNSAYGTGDKNNFCTEESALNPISIYAKEKVVIEKELMQREN
                     AISFRLATVFGMSPRMRIDLLVNDFTYRAVYDRFVVLFESSFKRNYVHVRDVSRVFQH
                     AIENFDKMKGQIYNVGLSEANVSKRELCEHIQKQVPDFVFLDAPVGKDPDQRNYIVSN
                     AKIEATGFKPMYSLDAGISDLVKGYTMIKNTCYGNV"
     gene            complement(1131..>1549)
                     /locus_tag="VN039_10000"
     CDS             complement(1131..>1549)
                     /locus_tag="VN039_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46329.1"
                     /translation="LGSQEPFLLLNGDTYFRVDRKVLNAYALEHDADWCLSLFRTSEK
                     GRYMGIEVSPEGQIMSLRSGVEQGSRLANGGVYWVHPRVLSEAPSLGEKMSLEDDLFP
                     NAFAAGRRLFGIEFKGTFIDIGVPEDYHRAPTLLVG"
BASE COUNT          368 a          432 c          382 g          367 t
ORIGIN      
        1 cgtaaccagc acagaatgcc gaatcacgtc cggctcaatc ttagattgag gggtcatatc
       61 ttgagcatca ctaccggcac ttctcatgtt gcattctatt gagtggaagg ctcaatagcc
      121 ttcttcggta agagtgacat gttccagctt acacattccc atagcacgtg ttcttgatca
      181 tcgtatatcc cttaaccagg tcgctgatcc cggcatcgag ggagtacatc ggtttgaacc
      241 cggtggcttc gatcttggcg ttggagacga tatagtttcg ttgatccgga tccttgccga
      301 ccggagcgtc gaggaacaca aagtcgggaa cctgtttctg gatatgttcg cagagctctc
      361 tcttcgacac gttggcctcg gacagaccga cgttgtagat ctggcccttc atcttgtcga
      421 aattttcaat ggcatgctgg aacacgcgtg agacatcgcg cacatggacg taattccgct
      481 tgaaggagct ttcaaacaag accacgaagc gatcgtagac ggctcgatag gtaaaatcgt
      541 tgaccaataa atcgatccgc atgcgtggag acattccgaa caccgtggcc agccgaaaac
      601 tgatggcgtt ttcccgctgc atgagttcct tctcgatgac cactttttcc ttcgcgtaga
      661 tggaaatcgg attgagcgcc gactcctcgg tacagaagtt attcttatcc ccggtcccat
      721 aggcgctgtt ggtggtgggc atcaggacca tctgctgctt tgacaggagc ttcagcatga
      781 ggataatggc gtcatggttg acggttgtcg cacccaccgg atcctggctg cacatcggcg
      841 cccctaccag cgcggccaac ggaatgacaa tatcggtctt cttgatcagc ggaaccatca
      901 cgctctcgat gcggatatcg cctttcacaa ccgaaaagtt ggggtgatgg cacacgtggt
      961 tgaggctggc ttgcttatac atgaaactat ccagcaccgt aatcttgtgc cccaactgaa
     1021 gcagatccgg gaccataata gaaccaagat agccggcgcc accggtaacg agaatgttgt
     1081 aactcatcga cataccctct ttgctgatta atgaacagtg ccctgtcggc ctaacctacc
     1141 aacagagtcg gggcccgatg atagtcctcg ggcactccga tatcgataaa cgtccccttg
     1201 aattcaatgc cgaataatcg tcgtccggcg gcgaaggcgt tagggaacag gtcgtcttcc
     1261 aaagacatct tttcacccaa actcggagcc tcggacagta ctcgtggatg cacccaatag
     1321 actcccccgt tggcaaggcg ggagccctgc tcaacgccgg acctcagcga cataatctgt
     1381 ccctcaggcg acacctcaat gcccatgtat cgccccttct cactcgtcct gaataaggac
     1441 aagcaccaat ctgcatcatg ttccagggca taagcattta ataccttccg gtccaccctg
     1501 aagtaggtat ctccgttcag gagcaaaaac ggctcttgac tgccgagct
//
LOCUS       DATMTI010000628         3775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_277732, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000628 DATMTI010000000
VERSION     DATMTI010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277732"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="VN039_10005"
     CDS             <1..1328
                     /locus_tag="VN039_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HWV46330.1"
                     /translation="RLLQKDVVFRQLGLVIIDEEQWFGVKHKERLKQLRTQVDVLTLT
                     ATPIPRTLQMAMSSVRDLSIIDTPPAGRLAIKTEVIRFSDKAVRDAILRELGRGGQVY
                     FVHNRVETMERIGAWLHQLVPEARMVMAHGQMDARPLEAVMLKFVKREADVLIASAII
                     QSGLDVPNANTIIVNRADLFGLAQLYQLRGRVGRGGEQAYAYFLIPDEGTLTGDAQKR
                     LIAIQQFTELGSGFRIAAADLEIRGAGNLLGKQQSGHIAAIGLDLYMQMVEQAVQRLK
                     GHVMEEEPDPTLQFPVSAFIPEQYVADPHHRLTLYKRLTACGQVGELALLHGEIQDRY
                     GPPPEPVERLLEVMQLRIHAKRLHLASIEVHGQTAKVVFHPKTVIPETSIHRLMDQLK
                     KRLRFLSPASFEVQMRDGDWPALFSELNAILQSLDLCDTKTIKKEATAS"
     gene            1342..2250
                     /locus_tag="VN039_10010"
     CDS             1342..2250
                     /locus_tag="VN039_10010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWV46331.1"
                     /translation="MPRLMMPFRFPTFRRTYCLLGLLVGMLLGMGPVVSGCAERQEEP
                     VVALVNGRAITQAEFDLRWGELSKATRSRYEKEGGKRRFLDELITRELLMQEARRRGL
                     DHDDTIRDKTQRYKEQLILDELLKDKLQSKVEVTQAELDAYYEKHANQLLDPLKADVS
                     LMLLPNVYAARDLEAQMHRGGSFVKFAQRYSIDEKTKHKGGELGPYRKGLVPPEVDAV
                     IHTLKIGMVSAPIKTDNGYYLVMLTPLDEAIIQADLATQERLRQELLSDKRRKRFDEV
                     IADIRANAVVRFAGASRHITEDTGKR"
     gene            2323..3327
                     /locus_tag="VN039_10015"
     CDS             2323..3327
                     /locus_tag="VN039_10015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWV46332.1"
                     /translation="MKRVSLRSDRPVRFKLPLVLLVITTWLPAISEARLQDRIVAIVN
                     SELIMLSDVKREFETEHERLSREHRGNDLPQRLKTAEYMALTKLIERRLQLQEAKAKK
                     VEVSDLEVKQALEQMKRQGSPLDITNTNHVETIREQLLLMRVVDLHIRGNITVGDSEL
                     KRFYQEHRDRFALPEEYQLSQIIIQPRSSDGLAEALTKARRAMDDLKRGEKFEDVAMQ
                     YSDGANALQGGRLGWVRQGELIRVIEQAVAHLVPGGISNIIESPEGIQIIRMDDRKPK
                     QFRRYEDARREIQEMVYRQKSDDMYESWLIDLKNKAYIEIKFEQVADQTQPQPVRSTP
                     "
     gene            complement(3335..>3775)
                     /locus_tag="VN039_10020"
     CDS             complement(3335..>3775)
                     /locus_tag="VN039_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HWV46333.1"
                     /translation="AVNVFLVSTSDPDLSQFHLVDLPGYGFAKVSKSVRTQWGPLMED
                     YLVGRASLLGIVMLVDSRVVTDQDRQTIAWLRSIRRNPLVVATKVDKLKPNERVRTLR
                     QTHHALGLADGELLIPYSSETGDGRDRVWGALREVAGRRSTDIS"
BASE COUNT          891 a         1067 c         1073 g          744 t
ORIGIN      
        1 accggctgct ccagaaagac gtggtattcc ggcaactcgg cctcgtgatt atcgatgaag
       61 agcaatggtt cggtgtcaaa cataaagaaa ggctgaagca acttcgcacc caggtcgacg
      121 tcctgacatt aactgccacc cccattccgc gtaccctcca aatggcgatg tcgagcgtgc
      181 gggacctttc gatcatcgat actccacccg ccggacgatt ggcgatcaag acggaggtga
      241 tccggttcag cgacaaggcg gtgcgggacg ccatcctgcg tgaactcggc cgtgggggac
      301 aagtctactt tgtccacaac cgggtcgaaa ccatggagcg gatcggggca tggctgcacc
      361 agttggttcc cgaagcgcgt atggtcatgg cgcatggcca gatggatgcc agaccactcg
      421 aagccgtgat gctgaaattc gtgaagcgcg aggcggacgt cttaatcgcc tcggccatca
      481 tccaatccgg actcgatgtt cccaatgcca acaccatcat cgtcaatcgg gcggatctct
      541 tcggcctggc gcagctgtac cagttgcgcg gacgagtcgg tcgcggcgga gagcaggcct
      601 acgcctactt cctcatcccc gatgaaggca cgctcaccgg cgatgctcag aagcgattga
      661 tcgcgattca gcaattcacg gaactcggat cgggcttccg tatcgctgcg gcagaccttg
      721 agattcgagg agcagggaat ctcttgggca aacaacagtc gggccatatc gccgcgatcg
      781 gcttggatct ctatatgcaa atggtggagc aggccgtcca gcggttgaag gggcatgtga
      841 tggaggagga gcctgacccg accctgcaat tccctgtttc ggcgtttatt ccggaacagt
      901 atgtggcgga tccgcatcac cgtctgactc tctataaacg gctgacggct tgcggccaag
      961 tcggggagct ggccctgttg catggggaga tccaggaccg ttacggtccc cctccggagc
     1021 cggtcgaacg actgcttgag gtgatgcaac tcaggattca cgccaaacga ctgcacctgg
     1081 cctcgatcga ggtgcacggc cagacagcca aggtggtgtt ccacccgaag acggtcattc
     1141 ctgagacctc catccaccga ttgatggacc agctcaagaa gcggctacgg ttcctgagcc
     1201 cggcctcatt cgaagtccaa atgcgtgacg gcgattggcc ggcgcttttt tcggaactca
     1261 acgcaatctt gcaaagcctc gatctctgtg ataccaagac catcaaaaag gaagcgacgg
     1321 cctcttaatc gagagccacc tgtgccacga cttatgatgc ccttccggtt tccgaccttc
     1381 cgcaggacat attgtctact tggactgctt gtcggcatgt tgctcggtat ggggccggtg
     1441 gtttcggggt gcgccgagcg acaggaagaa ccggtcgttg ccttggtgaa tggacgagcc
     1501 atcactcaag ccgaattcga ccttcggtgg ggtgagctct ccaaggccac acgctcacgc
     1561 tatgagaagg agggtgggaa gcgaagattc ctggacgagc tcatcacccg agagttgctg
     1621 atgcaggagg cccgtcgccg aggtcttgat catgacgata cgattcggga caagacacag
     1681 cggtataaag aacagctgat tctcgatgaa ctgttgaaag acaaacttca atccaaggtc
     1741 gaggtcaccc aggccgaact cgacgcctac tacgaaaagc acgccaacca actattggac
     1801 cccttgaagg ccgatgtctc gttgatgctc ctccctaatg tgtatgccgc cagagacctt
     1861 gaagcacaga tgcatagagg cgggagtttc gtcaagtttg cgcagcgata ctcaatcgac
     1921 gaaaagacga aacacaaggg cggcgagctc ggaccctacc ggaaaggact ggttcccccg
     1981 gaagtcgatg ccgtcatcca cacgctcaaa attgggatgg tcagcgcacc gattaagacc
     2041 gacaacggct attacttggt catgctgacc ccgctggacg aagccattat ccaagccgac
     2101 ttggcgacgc aggaacggct ccgacaagaa ttactgtccg ataagcgccg caagcggttc
     2161 gatgaagtca tcgccgatat tcgggcaaat gccgtggttc gcttcgccgg agcctcccgt
     2221 catatcaccg aggacaccgg taaacgctag cctggctcga ttccacagat ttatctattt
     2281 cgtgtaaaat ggtccgctct tgcgctatcc ggtgaaagga tcatgaaacg cgtttcgctc
     2341 cgatcggatc ggccggtccg gttcaagttg ccgttagtct tgttggtcat cacgacctgg
     2401 ctcccggcca tttccgaggc ccgtttgcaa gatcgcatcg tcgccatcgt caattccgaa
     2461 ttgatcatgt tatcggatgt gaagcgcgag tttgaaacgg agcacgagcg cctttcccgc
     2521 gagcaccgtg gaaacgatct ccctcagcga ttgaaaacag cggagtacat ggccttgacg
     2581 aagctcatcg agcggcgatt gcaactgcag gaagccaagg ccaaaaaggt cgaggtgtcg
     2641 gacctggaag tgaagcaagc gcttgaacaa atgaagcggc aaggtagtcc cctcgacatc
     2701 accaatacga atcatgtgga gaccattcgc gagcagctcc tcctcatgag agtcgtggac
     2761 ctgcacattc gcggcaacat taccgtcgga gactctgagc tcaaacggtt ctaccaagaa
     2821 catcgtgacc ggttcgctct tcccgaagaa taccaactga gccagatcat cattcaaccg
     2881 cgctcctcgg atggattggc cgaggccttg accaaagccc gccgagccat ggacgacttg
     2941 aagcgaggag agaaattcga agatgtcgcc atgcagtatt ccgacggcgc caatgccttg
     3001 caaggaggac gactgggatg ggtccgacag ggggaactca ttcgcgtcat cgaacaagcg
     3061 gtcgctcact tagtgccggg aggaatctcg aacatcatcg aaagccctga gggaatccag
     3121 attatccgca tggatgacag aaagccgaag cagtttcgcc gatacgagga cgcacggcgg
     3181 gaaattcagg aaatggtcta tcggcagaag agcgatgaca tgtatgaatc ctggctcatt
     3241 gatctcaaga acaaagcgta catcgaaatc aagttcgaac aagttgccga ccagacgcag
     3301 ccacagcctg tgcgttcgac gccataggta gcgatcaaga aatgtctgtg gaacgtcttc
     3361 cggctacctc gcgcagagct ccccacaccc gatcccgtcc atccccggtc tccgacgaat
     3421 agggaatcaa cagttcgccg tcggctagtc ccagagcatg gtgcgtctgc ctcagggtgc
     3481 gcacccgttc gttgggtttc aacttgtcca ccttggtcgc cacgacaagg ggattccggc
     3541 ggatcgaacg gagccacgcg atggtctgac gatcttggtc ggtcacgact cggctgtcca
     3601 ccaacatgac gatcccgagg agcgaggctc gaccgacgag atagtcttcc atcaaaggcc
     3661 cccactgggt acggacggat ttcgacactt tggcgaatcc gtagccgggt aggtccacaa
     3721 ggtgaaattg cgagaggtcc ggatcggaag tcgagacgag gaacacattc acggc
//
LOCUS       DATMTI010000629         1848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_660334, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000629 DATMTI010000000
VERSION     DATMTI010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660334"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..431)
                     /gene="panD"
                     /locus_tag="VN039_10025"
     CDS             complement(87..431)
                     /gene="panD"
                     /locus_tag="VN039_10025"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HWV46334.1"
                     /translation="MLRSKIHRTTVTGAHLEYEGSLTIDQDLMEAAGILPYEAIVCSN
                     LNNGERFMTYAINGPRGKGEIILNGPTARKAAVGDQIIIFCYEYYGEEEIKKHAPKIV
                     RVNDKNRIVTVS"
     gene            complement(516..809)
                     /gene="gatC"
                     /locus_tag="VN039_10030"
     CDS             complement(516..809)
                     /gene="gatC"
                     /locus_tag="VN039_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWV46335.1"
                     /translation="MEITQQDVEKVAQLARLAVTPAEKETFAKQLTQILTHVDTLNRY
                     DTTGVEPTATVMGQANVFREDLVRPSLPSDKALVNAPEREADGFVVPKILEER"
     gene            complement(952..>1848)
                     /gene="glmS"
                     /locus_tag="VN039_10035"
     CDS             complement(952..>1848)
                     /gene="glmS"
                     /locus_tag="VN039_10035"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWV46336.1"
                     /translation="KYLFEEMVRTPVQVDIGSEFRYRDPLVGKNDLFITISQSGETAD
                     TLAAAREAKQKGARVVSIVNVVGSTLARESDGVLYTHCGPEIGVASTKAFTAQLTALY
                     LLALHFARVRNVMKVADGKAWLDRLVQLPVLVERVLQREAEIVAIAKRYYKKRNFLFL
                     GRGINYPIALEGSLKLKEISYIHAEGYAAGEMKHGPIALIDKDMPVVVLAPRDRLYDK
                     TVSNLMEVKARRAPVIAFVAEGERELGKIADAVFTIPDTHSLISPILFTIPLQLLAYH
                     IAVLRGADVDQPRNLAKSVTVE"
BASE COUNT          406 a          560 c          449 g          433 t
ORIGIN      
        1 atgaagggac atgaatttct cgctaccgac gggtgagatg aacacggacc accacgagcg
       61 tcatcgatcg gcctgtccac ggcttctcag ctcaccgtca caattcgatt tttgtcattc
      121 acccgaacga tcttgggtgc gtgcttcttg atctcttctt cgccgtaata ctcataacaa
      181 aagatgatga tctgatcccc gaccgccgct tttcgcgcgg tgggcccgtt taaaatgatc
      241 tcccctttcc ctcgcggacc gttgatcgca taggtcatga accgctcgcc gttattcaag
      301 ttcgagcaga caatcgcctc atacggcaaa atcccggccg cctccatcaa gtcttgatca
      361 atcgtcagac taccttcata ctcgagatga gcacccgtca ctgtcgtacg gtgaattttc
      421 gaacgtagca tttgtcgaaa catctatctt tccgctattc gtcaatcgcc gctcagcatt
      481 cgttgttgtc aacgtccgag tcggcggcct gccgattaac gctcctctag aattttgggt
      541 acaacaaacc cgtcagcttc ccgctcaggt gcattaacca aagctttgtc cgaaggcagc
      601 gatggccgca cgagatcctc gcgaaacaca tttgcttgcc ccatgacggt ggcggtcggc
      661 tcgactcccg tcgtgtcgta ccggttcaac gtatcgacat gcgtgagaat ctgagtcaac
      721 tgtttggcaa atgtctcctt ctcggccggc gtgacagcca atcgtgccaa ctgtgccacc
      781 ttctccacgt cctgctgtgt aatctccact ctttttcctt ccccaaaaga tgttcaaaat
      841 ggtcttccag caaggccgca agaagtctcg cgactgaggg gtactttctg aagtacctcg
      901 cagggagaga gacgactgag aacgccgctg gagaccattt tcaacatccg cctattccac
      961 cgtgacgctc ttggccagat tcctcggctg atcgacatcc gccccgcgca ataccgcaat
     1021 atgatacgcg aggagttgca gcgggatggt aaacaaaatc ggcgagatca gcgaatgagt
     1081 gtcgggaatg gtaaacaccg catccgcgat cttgccgagc tcccgctctc cttctgctac
     1141 gaaggcaatc accggcgcgc ggcgcgcttt cacttccatg agattgctga ccgtcttgtc
     1201 gtagagtcga tcccgaggcg ctagaaccac gaccggcata tctttgtcga tcagcgcgat
     1261 cgggccatgc ttcatctcac ccgccgcgta gccttcggca tggatgtagg aaatttcctt
     1321 gagcttcagc gagccctcca gtgcgatcgg gtagttgatg ccgcgtccca aaaacagaaa
     1381 atttcgcttt ttgtaatacc gcttggcgat ggctacgatt tcagcttctc gctggagcac
     1441 acgctcgacc aacaccggca actgcactaa ccgatcgagc caggctttcc cgtccgccac
     1501 cttcatcaca ttacgaactc gggcaaaatg caaggccagg agatacagtg cggtgagttg
     1561 cgcggtaaat gccttggtag aagcaactcc gatctccggt ccgcagtgcg tgtacagcac
     1621 cccgtccgac tcacgcgcca aggtactgcc gacgacgttg acgatcgaga cgacgcgcgc
     1681 acctttttgc ttcgcctctc gcgcagcagc cagcgtatcg gccgtctcac cggattgaga
     1741 aatcgttatg aacaagtcgt tttttccgac gagcggatcg cgatatcgaa actcgctgcc
     1801 gatatccacc tgaacagggg tccggaccat ttcttcgaac aaatactt
//
LOCUS       DATMTI010000630         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_830883, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000630 DATMTI010000000
VERSION     DATMTI010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830883"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(629..1378)
                     /locus_tag="VN039_10040"
     CDS             complement(629..1378)
                     /locus_tag="VN039_10040"
                     /inference="COORDINATES: protein motif:HMM:NF039494.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWV46337.1"
                     /translation="MPNTIQHYTYAQLEALWIQAGGDRASAPIMAAIALAESSGIPTN
                     TNKNTDGTIDRGLWQINSSNVDSVTMFDPMANAKEAVKLKNNGQGLRNWVTYTNGAYR
                     KFLQNGVDPNGTGLPSGSTSTATDASLSGDLGNAIGQGLGNAFQALFQPLISMFIWGG
                     EIILGIGLVVGGLIVFSMNTSAGQAVKNAAGKSAEVAEPELSPELEARRREIQIMREQ
                     RTQARRKAIRQEASRRNKEIEKNGKRESSNS"
     gene            complement(1378..>1606)
                     /locus_tag="VN039_10045"
     CDS             complement(1378..>1606)
                     /locus_tag="VN039_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46338.1"
                     /translation="WFTVSSPFFHEQEAKDGNIKRIRWGEAAATAIVVATGWAVSNAT
                     GRGTPLMAALGISAVFVGGYEYMIAHPASEG"
BASE COUNT          359 a          457 c          409 g          381 t
ORIGIN      
        1 caaccatctt tgcgttttcc ggaatacctt cgacgcgtac gtagtcatac attgtcctca
       61 tgtctcacgg aaacctttca cgagaatcag agtgccgata accatgagcg ctacggcaat
      121 cccgatttca ccgaaccaca caatggcaga catgaaccct ttgaatacgg gtgctacgcc
      181 tgtaaagaat cctgctgtca cggaatcagc gcttaccggt tgagcgttgg tcgtgtcagc
      241 gactgaagtt gccccggacc atggtccgag tgacgacgtt gcctctttcc cctgaaagaa
      301 tgggaatgcc caacccaggt atgggtggcc ccatttgtcg gtaggcgtac gtgcaggtat
      361 ctgtcctaca gtaccggggc ccgtagcgtc tgtagaaatg ataagcccgt tacccaggga
      421 tagcgcaaca tgtccggccc cagtacttcc gccagaccag tacatgaggg cccccgccgg
      481 ggcattccag tccccggggt gacgcaagcc actaggtgtc gcctgccaat tcgttacggc
      541 tgttgaatat ccggatgacc cgtaaccgta tatctctgca acaaactcgt cacatcgacc
      601 aacggcgtaa cgcgtgaagg tgcgcgcgtt agctattgct tgattcgcgc ttaccatttt
      661 tctcgatttc cttgttacgc cgactggctt cctggcgtat cgcctttctg cgcgcttgcg
      721 ttcgctgctc tctcatgatt tgaatttcac gtcgtcgcgc ttcgagttct ggcgacaact
      781 ccggttctgc tacttcagca ctcttaccgg cagcattctt gacagcttgt ccagcactcg
      841 tattcataga aaagacaatg aggccaccga cgaccaggcc aatgccaagg atgatttcac
      901 caccccatat gaacatggat atgaggggct gaaacagcgc ctgaaacgca ttgcccagac
      961 cctgaccgat ggcattaccc agatctccgg aaagactggc atccgtagcg gtggacgtcg
     1021 acccggacgg cagtccagta ccgttcgggt caacaccgtt ctgaagaaac ttacggtatg
     1081 cgccattggt gtatgtaacc cagttgcgca acccttgccc attattcttt agctttacag
     1141 cttccttggc attcgccatg ggatcgaaca tagtgacaga atctacgtta cttgagttga
     1201 tttgccacaa gccacgatca atggtgccgt cggtgttctt attggtgttg gtaggaatac
     1261 cgctcgattc agccagcgca atggcagcca tgatgggagc agatgcgcga tcgccacctg
     1321 cctgtatcca caaagcttcc agttgtgcgt acgtgtaatg ctgaatcgtg ttaggcatta
     1381 tccctcgctt gcagggtgag caatcatgta ctcgtatccg ccgacaaaga ccgctgaaat
     1441 tccgagagca gccatgagag gagttccccg gccagtggcg ttagagactg cccaaccggt
     1501 ggcaacaacg atggcggtag cggcagcctc accccatcgg attcgcttga tgtttccatc
     1561 cttggcttcc tgctcatgaa agaatggaga tgacacggtg aaccag
//
LOCUS       DATMTI010000631         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_150958, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000631 DATMTI010000000
VERSION     DATMTI010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150958"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            555..>1684
                     /locus_tag="VN039_10050"
     CDS             555..>1684
                     /locus_tag="VN039_10050"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HWV46339.1"
                     /translation="MRRLTTVASFFVITAVCGLVLANHPDLRQSRNAAHIEENHPNGS
                     SGARWRWQNPLPQGNTLRGVSLIDANTGTLVGDYGTIVRTTDAGNSWTIQSSGTKQTL
                     WGVFFTDTTNGTAVGENGTILRTTDGGAHWLAQISGTPLNLRAVSFTDSSSGTAVGEG
                     GVIVRTTNGGSTWAPQSSGTLETLFGVSFSNSSTGTAVGGTFGKSEIFRTTDGGEHWF
                     PQANPGTQFLFDVSFTDTSNGTAVGDSGTILRTTDGGNSWVPQASGTTKTLYGVSFAD
                     TNNGTICGFDLGGGPAIILRTTDGGDSWIETNYSLPQGANAFYGVSFSDANTGMVVGE
                     AGIVLQTTNGGEDWNSQPTVTFNYLYGVSFTDANTGTAVGYE"
BASE COUNT          431 a          445 c          473 g          335 t
ORIGIN      
        1 attcccagat gttaatacgg gaacggccgt tggagctggt ggaaccatct taagaaccac
       61 gaacggaggg accaactgga accctcaaag cagcggaacc acagcgcctc ttcacggtgt
      121 tgcctttcga gatgctaaca caggcacagc agtgggaaac aacggtgtta tccttagaac
      181 aactgacggc ggaagccact ggggacctca aacgagcaac acacccgatc atttgtggga
      241 tgttgcgttc tctgacataa acaacggaac tgccgtaggt gaagggggca cgatcgtaag
      301 aacgacggat ggtggaagta actggactgt agattttagc ggtaccttca acatccttta
      361 cgacgtttct ttcgttaatg ccaataccgg gactgttgtt ggcgatcttg gtaccattct
      421 caggacaaca ggagccgggc cgactccaac gccgacaccg cgagccactc cgaggccgcg
      481 cccgcgaccc actccacggg cgcgtcccaa tacttaggtc taacaagtac gtttaagagg
      541 ggaagaggga tcacatgaga agactaacaa cggtagcgtc atttttcgtt attacggctg
      601 tgtgcggctt ggtattggcc aaccatcccg accttagaca aagccgtaat gcggcacaca
      661 tcgaggaaaa tcatccaaac ggttcgtcgg gcgcgcggtg gcgctggcaa aacccgcttc
      721 cacaggggaa cactcttcgc ggcgtctcgc ttatagacgc aaacactggc acgcttgttg
      781 gcgattacgg tacaatcgtc aggaccaccg atgccggaaa cagttggacg attcagtcca
      841 gtggtacgaa gcaaacgtta tggggcgttt tcttcacaga tacaaccaac ggaacggctg
      901 tcggcgaaaa tggaacgatc ctgcgaacca cagatggcgg cgctcattgg ctggcgcaaa
      961 tcagtggaac gccgctgaac ttgcgcgccg tttcatttac cgatagcagc agcggcacgg
     1021 ctgttggcga aggcggcgtc atcgtcagaa ctacaaacgg aggaagcacg tgggcgcctc
     1081 agtcgagcgg cactttggaa actctgtttg gagtgtcgtt tagcaactcc agcacaggaa
     1141 ccgcggtagg tggaacattc ggtaagagcg aaattttcag gaccacggat ggcggcgaac
     1201 attggtttcc tcaggcaaac ccagggacgc aatttctttt tgatgtctcg ttcaccgaca
     1261 cgagcaatgg aacggccgtg ggcgacagcg gcacaattct aaggacaaca gacggcggaa
     1321 atagctgggt acctcaggcc agtgggacca ccaaaacgtt atatggcgtt tccttcgccg
     1381 acacgaacaa cggtaccatc tgtggcttcg acttaggcgg tggcccggcc attattctca
     1441 ggacgacaga cggcggagac agctggatag agaccaacta ctcgctcccg caaggagcaa
     1501 acgctttcta cggagtgtcc ttcagtgatg cgaacaccgg aatggtcgtc ggcgaagctg
     1561 gtatcgttct tcagacaacg aacggcggcg aagattggaa tagtcagcca accgtcacat
     1621 tcaattatct gtatggcgtt tccttcaccg atgcgaacac cggcactgcc gtaggttacg
     1681 aaaa
//
LOCUS       DATMTI010000632         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_788109, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000632 DATMTI010000000
VERSION     DATMTI010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788109"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..736)
                     /locus_tag="VN039_10055"
     CDS             complement(23..736)
                     /locus_tag="VN039_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWV46340.1"
                     /translation="MLSGLAKCVICGGSIVACKNVSKRSTRRGSYQCALNHKRGTAIC
                     SNNVRLGLDVLDSAILHALNDAIDERVLETSVLTALNRIRAKQDSFPNQRLAIQRELS
                     LIEARCRRLLDLMGDGKGNPLMVKELDGLAARQAQLQQELQRLEAQTQVVHLDAKRLA
                     QDLRRRLSDLPSLFGRHVPQARQMLRKVLDGHILCEPILEDGKPGYRFSATGTFDRLL
                     TGKRVMNGRTNDRGCGERI"
     gene            complement(818..>1525)
                     /locus_tag="VN039_10060"
     CDS             complement(818..>1525)
                     /locus_tag="VN039_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWV46341.1"
                     /translation="LMNVLKPKPPFQVLIMSEESRLGREAIQVGYAFKQIIDAGVRVF
                     FYLTDQERKLDNALDKVMLSLTNFAAELEREKASQRTHDALHRKAKALYVTGNKVYGY
                     DNVPVYGDTLNADGTPKRQHVVRRINPEQAKIVIRIFESFASGVGLTALAKTLNEEHV
                     SPPHGGNRGWCPTAIRDMLRRDLYRGISWWNRTQMIQRGGTRKLRKRPQSEWLRVDAP
                     ELRIISDLLWEQVAERH"
BASE COUNT          312 a          456 c          417 g          340 t
ORIGIN      
        1 tcataaccca aaggtcagag gttcaaatcc tctccccgca accacgatcg ttggtcctgc
       61 cattcataac tcttttcccc gttaacagcc gatcaaaggt tccggtcgca gagaagcgat
      121 agcctggttt tccgtcctct aggatcggct cacacaggat atggccgtca agcaccttcc
      181 tgagcatctg gcgggcctgt gggacgtgcc gaccgaacag gctaggcaga tcgcttaacc
      241 gccgccgaag gtcctgggcg aggcgtttgg cgtccaagtg gacgacttgt gtctgggctt
      301 ccaggcgttg cagttcctgc tgcagctggg cttgtcgggc cgccagtcca tcgagctcct
      361 taaccatgag cggattgccc ttgccatccc ccatgagatc gagtagccgc cggcaccggg
      421 cctcaatgag agagagttcc cgttgaatgg ccaggcgttg attaggaaag gagtcctgct
      481 tagcccgaat gcgattcaga gccgtgagaa ctgaggtttc gagcacccgt tcgtcaatcg
      541 cgtcattcag ggcatgcagg atagccgaat caagcacatc caatccaagc cgtacattgt
      601 tggaacaaat cgccgtgcct cgcttgtggt tgagagcgca ctgataggaa ccccgccggg
      661 tcgatcgctt actgacattc ttgcaggcaa caatagaccc gccacagatg acgcatttcg
      721 cgagaccgct gagcaggtac gcgcttcgtt ggtcctcacc gctcggacgt gaaagtaagc
      781 ggccattgac tccatgaaca taggccgctt cgttccgtca atgtcgttcg gcgacctgtt
      841 cccacagcag atccgagata atccgcagtt ccggcgcatc aactctcagc cattcggact
      901 gtgggcgttt ccgtagcttg cgcgtgcctc cgcgctggat catctgcgtt cgattccacc
      961 aggagatgcc ccggtagagg tctcgccgca acatgtcccg tatggccgtg ggacaccatc
     1021 cccggtttcc gccgtgtggg ggagagacat gctcttcatt caacgttttg gcgagggcag
     1081 ttagacccac accggacgcg aacgattcaa agatacgaat gacgattttc gcctgctctg
     1141 gattgatccg gcggacgaca tgctgccgct tgggcgtacc atccgcattc agggtgtccc
     1201 catagaccgg gacgttatcg tagccataga ccttgttgcc ggtcacatag agggccttcg
     1261 ccttccgatg cagcgcatcg tgtgttcgtt ggctggcctt ctcgcgttcg agttctgccg
     1321 cgaagttggt cagactcagc atcaccttgt ccaaggcatt atccagctta cgttcttgat
     1381 ccgtcagata aaaaaagacg cgcaccccag catcaatgat ctgcttgaag gcatacccca
     1441 cctgaatggc ttcacggcct aaccgcgact cctccgacat gatgagcacc tggaacggtg
     1501 gcttcggctt gagcacgttc atgag
//
LOCUS       DATMTI010000633         1983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_108180, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000633 DATMTI010000000
VERSION     DATMTI010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108180"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VN039_10065"
     CDS             complement(<1..788)
                     /locus_tag="VN039_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HWV46342.1"
                     /translation="MDAEILRSVSRSFYLSIRFLPASLRDPIALAYLLARTTDTVADT
                     ASTPTEVRMETLRLLSDGVQGTASRDVVIKLVASFVSLQDNKSEQRLLESLPDHLVSL
                     EAMEHADRDDVRVVLGKITRGQMLDLERFDNPQEIRALGTAADLDDYTYLVAGCVGEF
                     WTRLCFRHIRQFASRTEDEMLALGKRYGMALQLVNVLRDAGSDLQAGRCYFPEHELSV
                     LHLTPSQILSESERFQPIYRTWLEKAKTGLASGMEYSRAIKNRRV"
     gene            complement(778..1734)
                     /locus_tag="VN039_10070"
     CDS             complement(778..1734)
                     /locus_tag="VN039_10070"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HWV46343.1"
                     /translation="MYAIRNPDAIKQYKTNPRVVREMVNRLVIAATGQPDVAKAWGSL
                     VSPSDRVGIKISAAGGDLFTTHHDVVNAIVDGLAAAGHSRSSIIVWDRSVGGIKEAGY
                     RKGVDGYQVKAIPPHDGYDPKAILTAPIVGKLVWGDYEFVGDIGKMPLLAEADATSNV
                     SHFAKIISSEVDKIINVPVMSVSETNGIAGCIYNMTIPNIDNWRRFAQGSRFGAGSLA
                     EIYANPLIAKKVVFNLMDGLIAQYAGGPQPQPNYAVHNATLYASKDPVALDAVALKKL
                     EEWRKRETLRPVAPVAAYIDVASQLGLGNSATNRIEVRNIGR"
BASE COUNT          468 a          555 c          508 g          452 t
ORIGIN      
        1 acccgacggt ttttgatcgc gcgcgaatat tccatccctg acgcaagacc ggttttcgcc
       61 ttctccaacc atgttcgata gatcggctgg aaccgctccg attcagagag gatctgtgat
      121 ggagtcagat ggagcacgct caattcatgt tcggggaaat aacatctccc ggcttgtaga
      181 tctgagccgg cgtcgcgcag cacattaacc agttgaagag ccatgccgta acgttttccc
      241 aacgcaagca tctcatcttc agtccgactg gcgaactggc ggatgtgccg gaaacagagc
      301 cgggtccaaa actcgccgac acaaccagcc acaagatatg tgtagtcatc gaggtctgca
      361 gctgtaccaa gcgcccggat ttcctgtgga ttgtcgaagc gttccaggtc gagcatctga
      421 ccacgggtaa tttttccgag cacaacgcga acgtcgtcac gatccgcatg ttccattgct
      481 tccaaagaaa ccaagtgatc gggtaatgat tcgagaagcc tttgttcgct cttgttgtct
      541 tgaagcgaaa caaaagacgc caccaactta ataacgacat cacgcgaagc tgtgccttgg
      601 acgccatcgg acagcagcct caaagtttcc attcgcactt cggtgggagt gcttgctgtg
      661 tcagcaaccg tgtcggttgt tcgagcgagg agatatgcca gcgcgatcgg atcgcgcaac
      721 gacgccggta gaaatcggat cgacaggtaa aaggagcgcg agaccgagcg caaaatttca
      781 gcgtccaatg ttccttacct ctattcgatt ggtagccgaa ttaccaagtc cgagctggct
      841 ggcaacatct atgtaggctg ccactggggc gacaggacgg agagtctcgc gcttccgcca
      901 ttcttccagc tttttcaatg cgacggcgtc cagagcgacg gggtctttgc tcgcatacaa
      961 cgtcgcattg tgcactgcgt aattcggttg aggctgcggg ccaccggcat attgcgcgat
     1021 gaggccatcc atcaagttga acacaacctt cttggcaatc aggggattag cataaatttc
     1081 ggcgagactt cccgcaccga atcgcgagcc ctgggcgaac cttcgccagt tgtcgatgtt
     1141 cggaatagtc atgttgtaaa tgcaaccggc gatcccattc gtttcactca cactcatcac
     1201 cgggacgtta atgattttgt ccacctcgct ggaaatgatt ttcgcaaaat gactcacgtt
     1261 gctcgtagcg tcggcctcgg cgagaagagg catcttccct atgtcgccaa cgaattcata
     1321 atcgccccac accagttttc cgactatagg cgcagttagg attgccttgg gatcgtaacc
     1381 atcatgcggc gggatcgctt ttacctgata gccatcaact cccttccgat atccggcttc
     1441 tttaattcca ccgacagaac ggtcccagac gataatgcta ctacgggaat gacccgccgc
     1501 cgccaggcca tccacaatcg cgttcaccac atcatggtgc gttgtgaaca ggtccccgcc
     1561 cgctgccgaa atcttgatac cgacgcggtc actcggagaa accagcgatc cccatgcttt
     1621 agctacatca ggctggcccg ttgcagctat aaccagtcga ttcaccattt cacgaaccac
     1681 acgcgggttg gtcttgtatt gtttgattgc atcaggattg cggatcgcat acacgatgga
     1741 tggtgtcggc gccggttgcg gaccttgcgc acgcccggat gcgatggcac aaaacaaaac
     1801 gagagctacc tttcgaagct ttgtcatgtc gagcggcgtc gggacatccc gcaaccttac
     1861 gtgaaagcga tgcaacggga ttcgtcgact ccgctcggaa tgacaacaga actgacgatt
     1921 ttatgagtaa cggcgtgaat cacaaccgtt cagaaatgaa aacggatcct gcagcgtgtt
     1981 tct
//
LOCUS       DATMTI010000634         6546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_277844, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000634 DATMTI010000000
VERSION     DATMTI010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277844"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1110)
                     /locus_tag="VN039_10075"
     CDS             complement(106..1110)
                     /locus_tag="VN039_10075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWV46344.1"
                     /translation="MCHDTRPYWERTGTSNANLSFRGELIGSRWKERLSCSINFEKAM
                     AHDWIDVCIQERFDAGELLSRLDDPTIQGAWEDDGVVHLYWPDDQWNETRLASVRLVL
                     ADLAPAIGDIPLSVRRVPGQNWNEAWARSVRPLHIGRLVIRPSWEPVVLGAKDVEIVL
                     DPKQAFGTGHHATTRILLGWLQKDIHGGEQVLDVGAGSAILAMAAVKLGAASAIGVEI
                     DSVAVDCAKEYIDQNGLKDRINIVCGTLADLPPERVKRADLVLANLDRQTILDLAGDL
                     VNSASEGSRIMVSGILVEQQDEIVERFSSFGLACSERSEEEGWIAMKFFRPESCDGEV
                     "
     gene            1102..2961
                     /locus_tag="VN039_10080"
     CDS             1102..2961
                     /locus_tag="VN039_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HWV46345.1"
                     /translation="MAHSRIALFETIVRRTDRLIARGIKTSATFTRWRLAIFLLGLAS
                     TITLYKLDWYQSGNLALGGFLAIFITVAAYHNRVETRIHRLRQWKQIKLAHLARMALD
                     WAAMAPKPGEAPKSHPYAADLDLFGAHSLTHLLDTSVSDHGRERLHSWLLEQPPTPSH
                     WHTRQSLVKELVSRSLFRDRLALEAKLTGDQEINGRRLAAVLEHPIGLPHLDTILIVQ
                     GLLALTTISLALATMFGQLPGYWMFSFAAYVLVYFMTDQGEELLEHAVGLHHETERLA
                     TVLGYIERHARRRNTALASVWTNRIGKASPTRHLNRAARALHAISIKAHPLVHLAINA
                     LCPWDLWFTRRLIQIQHEIKNALPLWLDSLAEVEAASALAAFAYLHPDYTWPTPLVAA
                     GERNGTPPAFHSDQLGHPLLPAKTRVANDVHLKGLGSIHLITGSNMSGKSTFLRTIGI
                     NLCLAQAGGPVCARSFEWTWSRLACCIRVDDSLDAGLSFFYAEVKRLKTILDATNDIT
                     APPVLFLIDEIFKGTNNRERLIGSRAYITELSQRNGFGLVSTHDLELADLEPAVPRLI
                     NAHFQETVSAGALEFDYRLRPGPCPTTNALRIMELEGLPISPVPNIRQNDPYG"
     gene            2958..6446
                     /locus_tag="VN039_10085"
     CDS             2958..6446
                     /locus_tag="VN039_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HWV46346.1"
                     /translation="MTTSAGSETQPLSHPLRGLLIAQFCGAFNDNAWKLMVALLAIRQ
                     ATAGMASGPALETVAQTQTAMAFVIFTLPLVFLSLVGGTLADRVSKRTVIIAIKIVEV
                     LLMAAGTVALWLNPAGGVLPLIVLCGMGVHSALFSPSKYGILPELIPHEQLAAGNGLL
                     EMWTFAAILTGTAAGGFLLQIAGAQTWLAPMALTGLSIIGLVTAFNIPQVSPARSAGG
                     VGTTLQGAWAAIQSERMLRMAIPMEVMFWTIASLFGQNVLVYAKAVLHLSDAMSGLPL
                     TILSVGIGIGAMLVGRISRNRVEYGLIPVGAIGVFLTLLSLGFLAPPLTETFFMMGVL
                     GIASSFIFVPLNAILQWKSPSDRRGAVISFSNTCVFTGILLGSLVGGSLANAGLSTTG
                     IFLAAAAMTLGGIGWALWFLPDTLIRLVLVILTNTLYRLRIVGQSHVPPSGGALLVPN
                     HVSFIDGFLLIASLDRPVRFVVDSQYAEQPIFKHFMKTLGVIPISSHGGLRVILKALR
                     DAGAAVDKGELICIFPEGQITRTGTLLPFRRGFERVVKGRTAPIIPVHLDRVWGSIFS
                     FNHGRFLWKVPEQLPYPVTVSFGTPLSPGTPAHDLRGKIQELGEAAWQFRKPSRQPLH
                     RQFIRSMRRYPFRLAMADQNRPRVSSLQALIGSIVLARTLRPYWKDQQRVGVLLPPTV
                     AAALVNVAAPLCGKTIVNLNYTVGKSGLEAAVEQADLRTIVTSRVFIEKAKLELPDGP
                     SVVWLEDVAATISNGQKVLAALLALFVPCRFIERACGQTIPLTPDSMATIIFSSGSTG
                     EPKGVMLSHFNIDANSQGATQVLHFYQDERVLGILPFFHSFGYMVFWFVMSNNAAIIF
                     HPSPLDVAAIGELIRRHQITFLVTTPTFLQLYSRRCTPEQFGSVRVILTGAEKLPTRL
                     AEAVEDKFGIGPIEGYGVTECSPVIAVNCPDFRAAGYYQPASRRGTVGQPLPGVSVQI
                     VDPDSYMPLPAGTPGMLLVKGPNVMNGYLGREDLTAQVMRGGWYITGDIAILDDDGFL
                     TITDRLSRFSKIGGEMVPHGKVEEALQQAAGSETQVFAVTGLPDEKRGERLAVIHTLD
                     EDLIPDILAKLSGSGLPNLFIPTRSQFVKVETLPVLGTGKLDLRGVKRLAMERLSSHE
                     TAELRSKEADQKSIDE"
BASE COUNT         1352 a         1989 c         1725 g         1480 t
ORIGIN      
        1 aaccccgttc gctcacctta gcctcccaaa ccggcaactt cggaacacct ccatcggaga
       61 tgttaatctg atgcacatgc ggatagggcg gcctggtcga catcgtcaca cctctccatc
      121 gcaggattcc ggcctgaaaa acttcatcgc tatccagccc tcttcctcgg atcgttctga
      181 acacgccaag ccgaaactgg aaaaccgttc gacgatttcg tcctgctgtt cgaccagaat
      241 gccggaaacc atgattctcg atccttctga cgccgaattt actaaatcac cggcaaggtc
      301 caagatcgtt tgcctgtcga gattggccag cacaagatca gccctcttca ctctctctgg
      361 tggcagatcg gccaatgtac cacacacgat attgatccga tccttcaacc cgttctgatc
      421 gatatactcc ttcgcgcagt ccaccgcgac agaatcaatc tcgacaccaa tggccgatgc
      481 ggcgccgagt ttgaccgctg ccatggccag gatggcgctg cccgcgccca catccaaaac
      541 ctgctctccg ccatgaatgt ccttctgcaa ccatccgagc aatatccgag tggtcgcatg
      601 gtggcccgtg ccgaatgctt gcttgggatc gagcacgatc tcgacatcct ttgcccccag
      661 cacgaccggt tcccaactcg gacgaatgac cagccgaccg atgtgaagcg gcctcacgga
      721 gcgagcccat gcctcgttcc aattctgacc cggtacccgt cttaccgaga gcggaatgtc
      781 cccgatcgct ggtgcgaggt ctgcaaggac caggcgtacc gaagcgagcc gagtttcgtt
      841 ccactggtca tccggccaat aaagatggac gaccccatca tcctcccaag cgccctggat
      901 ggtaggatca tcaagccgac tgagcaactc gcctgcgtcg aaacgctctt ggatgcagac
      961 gtcaatccag tcgtgagcca tggccttttc gaaattgatg gaacatgaca ggcgctcttt
     1021 ccaacgtgag ccgatgagtt cgcctctgaa actcagattg gcatttgacg ttcctgtgcg
     1081 ttcccagtaa ggtcgcgtgt catggcacac tcgcgtatcg ctctgttcga gacgatagtt
     1141 cggcgaacgg atcgtcttat tgccagagga atcaagacca gtgcaacgtt cacacgatgg
     1201 cgattagcca tattcctcct cggcctcgcc agcacgatta ccctctacaa gctcgattgg
     1261 tatcagagcg gcaacctggc cctcggagga ttccttgcca tcttcatcac tgtggcggca
     1321 tatcataaca gggtagaaac ccggattcac cggcttcgcc aatggaagca gatcaagctg
     1381 gcccatctgg ctcggatggc tctggattgg gccgcaatgg ctccaaaacc cggtgaagct
     1441 ccgaagtctc atccgtacgc cgctgacctg gatctcttcg gcgcgcactc tctaacacat
     1501 cttctcgaca ccagcgtgtc ggaccatggc cgagagcgtt tacatagctg gctgctggag
     1561 caacccccta ccccctcaca ttggcacaca cgccagtctt tggtgaaaga actggtatct
     1621 cgctccttgt ttcgtgaccg tctagcgctc gaagccaaac tgacgggaga tcaagaaatc
     1681 aacggcaggc ggttggccgc tgtgcttgaa catccgatcg gtctccccca cttggatacc
     1741 atccttattg tccaaggtct cctcgccctt acaacgataa gccttgcctt agccacgatg
     1801 ttcggtcagc tccccggcta ttggatgttt tcgttcgcgg cctatgtgct ggtctacttc
     1861 atgactgatc aaggggaaga gttgctggag catgccgtcg gccttcatca tgaaaccgag
     1921 cgacttgcca cggtcctcgg ctacattgag cgacatgcaa ggcgacgaaa cacggcgctc
     1981 gcttctgtct ggacgaaccg gatcggtaaa gcaagtccaa cacggcatct caaccgcgcc
     2041 gcacgcgcgc ttcatgcaat cagcatcaag gctcacccgc tcgttcatct ggcgatcaac
     2101 gcgctatgcc catgggatct gtggttcact cgacggctga ttcagattca gcacgaaatc
     2161 aaaaatgccc ttcctctgtg gttggacagc ttggcggaag tcgaagcggc ctcggcgttg
     2221 gctgcctttg cctatctcca tcccgattac acatggccga ctcccctcgt cgcagccggc
     2281 gaacggaacg gcacacctcc tgctttccac agcgaccaac tgggccatcc gttgctgccg
     2341 gctaagaccc gtgttgccaa cgacgttcat ctgaagggac tcggctccat tcacctgatt
     2401 accggctcca acatgtccgg caaaagcacc ttccttcgaa cgatcggtat caacctctgt
     2461 cttgcacaag ccggaggtcc tgtttgtgca cgctcgtttg aatggacgtg gagccggctg
     2521 gcctgctgca ttcgcgttga cgattcgctc gatgccggcc tgtcgttctt ctatgccgag
     2581 gtgaaacggc tcaagacgat cctcgatgcc accaacgaca taaccgcacc acccgtgctg
     2641 ttcctgatcg atgagatctt caaaggcacc aataaccggg agcggctcat cggcagccgc
     2701 gcctacatta ccgaactgtc gcaaagaaac ggcttcggcc tcgtgagcac tcatgatttg
     2761 gagttggcgg atttggagcc tgccgtgccg agactcatca atgcgcactt tcaagaaacc
     2821 gtgtcagccg gtgcactcga gtttgattat cggctccgcc caggtccttg ccccacgaca
     2881 aacgcgctga gaattatgga gttggaaggc ctgcctattt cccccgtacc aaatatcagg
     2941 cagaatgacc cttacggatg accacctcgg ccggttctga aactcaacct ctgtcacatc
     3001 cccttcgagg tctcttgatc gcccaattct gcggcgcctt caacgataat gcgtggaaac
     3061 tgatggtcgc tttgctcgcc attcggcaag ccacagcagg tatggcatcg ggtcccgcat
     3121 tggagacagt cgcccagacg cagaccgcga tggcattcgt cattttcacc ctgccgttgg
     3181 tgttcctctc tctcgtcgga ggcacattgg ccgatcgcgt gagtaagcgg acggttatca
     3241 tcgccattaa gatcgtcgaa gtgctcctca tggcggccgg cacggttgcg ctctggctga
     3301 atccagccgg tggcgtcctg cctttgatcg tcttatgcgg catgggtgtg catagcgccc
     3361 tgtttagtcc ttcgaagtac gggatactcc ccgaattgat cccgcatgaa cagcttgctg
     3421 ccggcaacgg cctcttggag atgtggacct ttgcggcaat tctgacggga accgcagcag
     3481 ggggattcct tctacaaatc gcaggcgctc aaacctggct ggccccgatg gcattgacag
     3541 gcctctcgat catcggcctc gtgaccgcct ttaacatccc acaggtgtca cccgcccgct
     3601 cggccggagg tgtgggaacc acccttcaag gcgcatgggc ggcaattcaa tccgaacgga
     3661 tgttgcgcat ggccattccc atggaagtca tgttctggac cattgcgagc ctcttcggac
     3721 agaacgtgct cgtctatgcc aaagccgttt tgcatctgtc tgatgcgatg tcgggccttc
     3781 cactcaccat cctatcggta ggcatcggaa tcggcgcaat gctggttgga cgaatctcaa
     3841 ggaaccgggt cgagtacgga ctgattccgg ttggcgccat cggcgtgttt ctcaccttac
     3901 tgtcgctcgg cttcctggcc ccaccgttga cagaaacgtt cttcatgatg ggagtgttgg
     3961 gtattgccag ttcattcatc tttgttccgt tgaacgccat tctccaatgg aaatctccgt
     4021 ccgatcggag gggtgccgtc atttcgttct ccaacacatg cgttttcacc ggcatcttgt
     4081 tgggatcact ggtaggcggt tccttggcca acgccggcct ctcaacaacc ggcattttct
     4141 tggctgcagc cgcaatgacc ctgggcggca taggatgggc gctttggttt ttgcctgaca
     4201 cgcttattcg gctggtgctg gtcattctca ccaacaccct ctatcggctc cgcatcgtcg
     4261 gccagtctca tgttccgccc tccggtggag cgctcctcgt ccccaatcac gtctccttta
     4321 tcgacggctt tctcctgatc gccagcttgg atcgacccgt gcgctttgtc gttgattcgc
     4381 aatatgccga acagccgatc ttcaaacatt tcatgaagac gctcggagtc attcccattt
     4441 cctctcatgg aggcttgcgg gtcattttga aagcgctccg cgatgccggt gcggccgtcg
     4501 acaagggaga actcatctgc atcttccctg aaggccagat tactcgtacc ggaacgttgt
     4561 tgccgttccg gcgggggttc gaacgggttg tgaaaggacg gacggctccc attatcccgg
     4621 ttcatctgga ccgtgtgtgg ggcagcatct tcagcttcaa tcacggccgc tttctgtgga
     4681 aagttccgga acaacttccg tatcccgtga ccgtctcgtt cggtacgccg ctgtcgccgg
     4741 ggacaccggc gcacgacttg cgcgggaaga ttcaagaact cggcgaagca gcctggcaat
     4801 tcaggaagcc gagtcgccaa ccactccatc gccaatttat ccggtccatg cgtcgctatc
     4861 ccttccgtct ggccatggcc gaccagaacc gtccccgcgt ctcatccttg caagctctca
     4921 tcggatcaat cgtccttgcg agaacgcttc ggccgtactg gaaggaccaa cagcgagtgg
     4981 gcgtcttgtt gccacccaca gtcgccgcag ccctggtgaa tgtcgccgcc cccctctgcg
     5041 ggaagaccat cgtaaacttg aactacacgg tcgggaaatc cgggctagaa gccgcagtag
     5101 aacaggcaga tctgcgcaca atcgtgacga gccgtgtctt cattgaaaaa gccaaactcg
     5161 aattgccgga tggaccgtcg gtcgtttggc tggaggacgt ggccgccacg atcagcaatg
     5221 gccagaaagt gctggctgcc ctgttggctc tgttcgttcc atgccgcttc attgaacgcg
     5281 cctgcgggca gacgattccg ctcacccccg acagcatggc cacgatcatc ttcagcagcg
     5341 gcagcacagg agaacctaaa ggcgtcatgc tttcgcactt caatatcgat gccaacagtc
     5401 agggtgccac acaagtgctc catttctatc aggacgaacg tgtcctcggc attttgccgt
     5461 tcttccattc attcggatac atggtgttct ggtttgtgat gtccaataat gcagcgatca
     5521 tctttcaccc gtccccgctc gacgtggcgg cgatcggcga actcatccga cgacatcaga
     5581 tcacttttct cgtcaccacg ccgacgtttc tgcaactcta ctctcgccgt tgtacgccgg
     5641 aacaattcgg ttcggtgcgg gtgatcctca ccggcgcgga aaaactgccg acacgactcg
     5701 cagaggccgt tgaagacaag tttgggatcg gaccgatcga aggctatgga gtcacggagt
     5761 gttctccggt cattgccgtc aactgtccgg acttccgcgc tgctggctac taccaaccgg
     5821 cctcacgccg aggcaccgtc ggtcagccgc ttcccggtgt gtccgtgcag attgttgatc
     5881 ctgatagtta catgccactc ccagccggca cacctggaat gttgctcgtg aaggggccga
     5941 atgtgatgaa cggctatctt ggacgcgaag atctcacggc tcaggtcatg cgaggtggct
     6001 ggtatatcac cggcgacatc gccatactgg acgatgatgg gtttctcacg atcacggacc
     6061 ggctatcgcg attctcgaag atcggaggcg aaatggttcc gcacgggaaa gtcgaagagg
     6121 ccttgcaaca agcggccgga tccgagaccc aggtgtttgc cgtgaccggt cttccggacg
     6181 agaaaagggg agagcggctg gccgtcattc atacccttga tgaggacctt attcctgaca
     6241 tcctggcaaa attgtccggc tccggcctcc cgaatctctt cattccgacg cggagccagt
     6301 tcgtcaaggt cgagacgtta ccggtcctcg gaaccggcaa gctggattta cgaggcgtga
     6361 agcgcttggc gatggaacga ctctcatcac atgaaacggc ggaactaagg agtaaggaag
     6421 cggaccagaa atcgattgac gaatgatgtt caccaaagat gagcggtgaa ggatcgtgaa
     6481 gtgatgaaga tgaataggag taagttgacg tttatctttg tataagaaac aaaaagggcc
     6541 gtcctt
//
LOCUS       DATMTI010000635         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_788325, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000635 DATMTI010000000
VERSION     DATMTI010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788325"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VN039_10090"
     CDS             complement(<1..511)
                     /locus_tag="VN039_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWV46347.1"
                     /translation="MSGVVYGILWVLVRIVGWVCFRYRVEGQVPQTGGVLIAANHASY
                     LDIPLLGCGMRRRAWYLGRNDLFPIPILNGILRALGWIPVRLGRLDREAFGKAINLIR
                     AGNAVVIFPEGGRSHDGHLRPPKAGIGVIVSQTGCPVVPAYLKGTFDVLPMGAHWPRL
                     RQITVRFGDP"
     gene            complement(508..1167)
                     /gene="cmk"
                     /locus_tag="VN039_10095"
     CDS             complement(508..1167)
                     /gene="cmk"
                     /locus_tag="VN039_10095"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWV46348.1"
                     /translation="MIIAIDGPAGVGKSTVAKLLAARLGYLYLDTGALYRAIGWKTLQ
                     SRIRPTDYEQVATLLPTTSIHMQFHHDTMQVLVDGIDVTGELRTPEVTATASIVSAIP
                     AVREWLLPIQRQIGQRGSVVAEGRDIGTKVFPAAPFKFFLEADADVRVARRHSELVGA
                     GRGGTVETTFRDLSDRDRRDRTRSVDPLVPAGDARLIDTSALSPDQVVEQMIVAVSTG
                     S"
     gene            complement(1213..>2484)
                     /gene="aroA"
                     /locus_tag="VN039_10100"
     CDS             complement(1213..>2484)
                     /gene="aroA"
                     /locus_tag="VN039_10100"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWV46349.1"
                     /translation="VPGDKSLTHRAIILTALAEGTSTIEAYWQGEDCLNTMRAFQRLG
                     IPITQTPTELTVCGKGFWGLSEPSAPIDCGNSGTGIRLLTGLLAGQDFFSVLTGDESI
                     RRRPMGRVVKPLREMGAIIGGRKGGELAPLAMTGACLHGIEYTSPVASAQIKSSLLLA
                     GLFAQGKTRYKEPSLSRDHTERMFQFFGVPLATEEGALVLQGRPSVGWRGVHMTIPGD
                     FSAAAFFIVGATIVQGSDVTIRNVGMNPTRTGLIDVLRKMGADIQILGLREAAGEPVG
                     DLRVKAAPLKGVTIGHDLIPKTIDEFPVLCVAAAVADGDTVISGAGELRVKESDRIAT
                     MSRELKMMGALLEEQPDGMIIRGLGQGGENGRLKAAGKVESHGDHRVAMSLAIGGLTA
                     EESMTIADTSCVDTSFPNFEKALVDLLGRPA"
BASE COUNT          546 a          790 c          622 g          526 t
ORIGIN      
        1 taggatcacc gaatcgtacc gtgatctgac gcaaccgagg ccagtgtgcc cccatgggga
       61 gtacgtcgaa ggttcccttc agatatgctg gaacgactgg acatcccgtc tgcgacacaa
      121 tcactccaat acccgccttc ggaggtcgga ggtggccatc gtggctgcgt ccaccttccg
      181 ggaatatgac cacggcatta cctgctcgaa tcaggttgat cgcctttcca aacgcctctc
      241 ggtcgaggcg ccccaacctc accggtatcc agcccaatgc ccgcaaaatt ccgttcaata
      301 tcggaatcgg aaacaagtcg ttccgtccca agtaccaggc tcttcgacgc atcccgcagc
      361 cgagcagggg aatatcgaga tagctggcat gatttgcggc gatcagcacc cccccggtct
      421 gaggaacctg accttcgaca cgataccgaa agcaaaccca tccaacgatt cgtaccaaaa
      481 cccacaaaat tccatagact accccgctca cgacccggtc gacacagcca cgatcatctg
      541 ctccaccact tggtcagggc tgagagcaga ggtatcgatg agtcgcgcgt cacccgctgg
      601 aaccaatggg tcgaccgaac gggttcgatc acgccggtcc cggtccgaca gatccctgaa
      661 cgttgtttca accgtcccac ctcgacccgc accgaccagc tcactgtgcc gccgtgcaac
      721 tcgtacgtct gcgtccgcct ccaaaaagaa tttgaatggt gctgcgggaa agaccttggt
      781 accgatgtcc cgtccctctg cgacgaccga gcctctctgg ccgatctgac gttggatcgg
      841 caacagccat tcgcggactg ccggaatagc ggacacgatg gaggctgttg cggtaacttc
      901 tggcgtacga agttctccgg taacgtcgat gccatcgacc aagacctgca tcgtgtcatg
      961 gtgaaactgc atatgaatcg aggtagtcgg caataatgtg gccacttgct cataatcagt
     1021 cggacgtatc cttgattgca aggttttcca tccgatggct cggtacagtg cccctgtatc
     1081 aagatacagg tagccaaggc gagccgccag taatttggct actgtactct tgccgactcc
     1141 ggccggtcca tcaatcgcaa taatcactca gcgcctctca atttcgaact gtcccatccg
     1201 acacacccgc tttcacgcag gccgccccaa cagatcgacg agtgccttct caaaatttgg
     1261 gaatgacgta tccacgcaac tcgtgtcggc aatcgtcata ctctcttcgg ccgtcaggcc
     1321 accgatggca agagacatgg ccacgcgatg atctccatgg ctctcgacct tccccgcagc
     1381 tttcaatcgg ccgttctctc ctccttgacc taagccgcgg atgatcatcc cgtccggctg
     1441 ttcctcgagc agagctccca tcatcttcaa ctcgcggctc atggtggcaa tccgatcact
     1501 ctctttgacc cgaagttccc ctgctccgga aatcacagta tccccatccg ccacagccgc
     1561 agcaacgcac agcacgggaa attcatcgat cgttttggga atgaggtcat ggcctatcgt
     1621 gacgcccttc agtggggcag ccttcacacg aagatccccg accggttcgc cggccgcttc
     1681 tcgcagaccc agaatctgaa tgtcggctcc catctttctc aggacatcga tgaggccggt
     1741 tctcgtcgga ttcatcccga cattgcgaat ggtgacgtcc gatccttgca caatcgttgc
     1801 tccgacgatg aagaatgcgg cggccgagaa atctccggga atagtcatat gaacgcctct
     1861 ccatccgact gagggtcgcc cttgtaagac caaggcacct tcttctgttg cgagagggac
     1921 tccaaagaac tgaaacatcc gttcagtatg gtctcgtgat agacttggtt ccttataccg
     1981 agtctttccc tgagcaaaga ggccagcgag caggagggac gacttaatct gcgcgctggc
     2041 cacgggcgag gtgtactcga taccgtgcag acaggctcct gtcatcgcca aaggagccag
     2101 ttctcctcct ttacggcctc caatgatggc acccatttcc cgcagcggct taacgacccg
     2161 acccataggt cgccgtctga tcgattcatc gcccgtgaga accgagaaaa aatcttgccc
     2221 ggccaaaagt cccgtgagca gacggatacc ggttcctgaa ttgccgcaat cgatcggagc
     2281 gcttggttcg gataaccccc aaaacccctt cccgcagacg gttaattcgg ttggagtctg
     2341 cgtaatggga atccccagtc tctgaaaggc cctcatggtg tttaggcaat cctctccctg
     2401 ccagtaggcc tctatcgtgc tcgttccttc cgccagcgcg gtgaggatga tcgcccgatg
     2461 ggtgagagac ttgtcgccgg gaac
//
LOCUS       DATMTI010000636         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_151233, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000636 DATMTI010000000
VERSION     DATMTI010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151233"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="VN039_10105"
     CDS             <1..1719
                     /locus_tag="VN039_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWV46350.1"
                     /translation="DRERINRAITLSLETGRLFSEEYRTVWPDGTVRWLSDRGRVYRD
                     QTGKPARMIGILLDITLRKDAEEAMRDWTTKLEQRVKERTTELTQANERFDWVARATN
                     DGVWDWDLVHDTIYLSPRWKVMHGFQESDGPELTKEWRTRIHPEERPSVVASLERYLR
                     GEQGDFWEEEYRIQRKDGDYFWVLDRSIAIRDEQGRAVRMVGADTDISWRKGIEQALR
                     LREQQFHTLADNVPALFSYVDLDRRYQFLNKRYEELFDRGDDELRGMSVENLLGSDGY
                     AQVRPFLDQALEGQSVSFEYTLQLPGNDTRHLVGQYVPDRNDQGQVLGVFVLMTDVTA
                     LKTTEGLLREREAQLRELGTRLLQAQEEERRRISRDLHDDVMQRMGGLTLELYGLASS
                     TSLQDVRLQSQLTSCGASAEQLTTDLQRIAHQLHPSVLEFGGLETAVRDHVNEFAART
                     GISAELMIHKVPKDIPLDQATCLYRVLQEGLQNVQKHAEATTVLVRLLRTRRGVGLCI
                     HDDGCGIENSDGAARRRGLGLTSMAERVGMLNGTFRILTNQNDGTELHAWVPLELKRM
                     AMARGS"
     gene            1836..2483
                     /locus_tag="VN039_10110"
     CDS             1836..2483
                     /locus_tag="VN039_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV46351.1"
                     /translation="MADDHSIMLAGLRKLVEGICDVVGAVEDGRALVEAAERLQPDLV
                     LVDISMPLLNGMDAARQLKKSIPNAKLIFLTMHASRVVAAEALQAGANGYLLKQSAAS
                     ELPLAIEEVLKGNTYLTPTITRPVLNAMVTRNRTELRTSLMQLTPRQREVLQLLAEGK
                     SPKDIAVLLNVSVRTVDFHKTRLMQQLDLHSTLALAKFAIAEGLVSADTGLSPQS"
BASE COUNT          589 a          674 c          843 g          520 t
ORIGIN      
        1 gatcgggaac gaatcaatcg ggccattacg ctgtctcttg aaaccggtcg gctattcagt
       61 gaggaatatc gaacggtctg gcctgacggg acggtgcgct ggctgagcga tcgtggcagg
      121 gtataccggg atcagaccgg caagccagca cgcatgatcg gcattcttct agacataacg
      181 ctgcggaagg acgccgaaga agcaatgcgc gactggacca caaagctgga acagcgtgtg
      241 aaggaacgga cgactgaact gacacaagct aacgagcgct ttgattgggt ggcgagggcg
      301 accaacgacg gcgtgtggga ttgggatctg gtgcacgata ccatatacct ttctcctcgc
      361 tggaaggtga tgcacgggtt tcaagaaagt gatggaccgg aattgacgaa ggaatggcgg
      421 acgcgcattc atccggaaga gcggccatcc gtcgtggcca gtcttgagag gtatcttcgc
      481 ggcgagcagg gagacttctg ggaggaagaa taccgcatcc aacggaaaga cggcgactat
      541 ttctgggtgc tcgaccggag cattgccatt cgcgatgagc aggggcgagc ggtccgcatg
      601 gtcggtgcgg acacggacat ctcctggcgc aagggaatcg agcaggctct gcgtctcaga
      661 gagcagcagt tccacacgct ggccgacaat gtgccagccc tcttcagcta tgtcgatttg
      721 gatcggcggt accagttcct gaacaaacga tatgaagaac tcttcgaccg gggcgatgat
      781 gagttgaggg gcatgtcggt ggagaacctc cttggatcag acggatatgc gcaggtccgg
      841 cctttcctgg accaagccct cgaaggacaa tcggtttcgt tcgagtacac attgcagttg
      901 cctggaaacg acacacggca cttagtcggt cagtatgttc cggataggaa tgatcaaggg
      961 caagtcttag gggtattcgt gctgatgaca gatgtgacgg cgctcaagac gaccgagggg
     1021 ctgttgcgag agcgggaagc gcagctccgc gagctgggga ccaggttgct gcaggcgcag
     1081 gaggaggaac ggcggaggat ttcgcgggat ctccatgatg atgtgatgca acgaatggga
     1141 ggcctgacgt tggagctata cggcctggct tcttcgacct cattgcaaga tgtgagattg
     1201 caatcgcagc tcacctcttg tggggcatca gcggagcagc tgaccacgga cttgcaacgg
     1261 atcgcgcatc agctccatcc ttcagtcttg gaatttgggg gactggagac agcggtgcgc
     1321 gatcacgtca acgaattcgc cgcgcgaaca ggcatatcgg cggaacttat gatccataag
     1381 gtccccaagg atatcccact cgaccaagcc acctgtctat atcgcgtgtt gcaagaaggg
     1441 cttcagaatg tgcagaagca tgcggaagcg accaccgtat tggtgcggct cttgcggacc
     1501 aggcgaggcg tggggctatg tatccacgat gatggatgtg ggatcgaaaa ttccgacgga
     1561 gccgctcggc ggagggggct cggcttgacc agcatggcgg agcgggtggg gatgctgaac
     1621 ggcacgtttc gcatcctgac caatcagaat gacgggacag agctccacgc gtgggtgccg
     1681 ctggagctga agaggatggc catggcgcgt ggatcgtgac gcgcgcgtga gggcaagcga
     1741 cggcaattag catagcagct tgcaccgtgt ttgaaatgcc agcataatgg agtatgatgc
     1801 ctgagataac gacgagaagg aagccccgcc tgctgatggc cgatgatcat tcgatcatgt
     1861 tggccggcct ccggaaattg gtggagggca tctgtgatgt cgtcggggcg gtcgaagatg
     1921 gccgtgcctt ggttgaggcg gctgagcgac ttcagccgga cctggtcctg gtggacatct
     1981 ctatgccgct cctgaacggc atggacgcgg cgcgacagct caagaaatcg ataccgaacg
     2041 cgaagctcat ctttctgacg atgcatgcct ctcgggttgt tgccgccgaa gcgctccagg
     2101 ctggtgccaa cggctacctc ctgaagcagt cggcggcttc tgaactgccg ctggcgatcg
     2161 aagaagtgct gaaggggaac acctatctca cgccgacgat caccagaccg gtcctcaatg
     2221 cgatggtgac gaggaatcgg acggagctga ggacatcgct gatgcagttg acgcctcggc
     2281 agcgggaggt gctgcagctc cttgcggaag gaaaaagccc caaagacatc gccgtcctct
     2341 taaatgtatc tgttaggacc gtcgacttcc acaaaacgcg gctgatgcag caactcgacc
     2401 tccattctac gctcgcactc gccaaattcg ccatcgccga agggctagtt agtgccgaca
     2461 ccggcctttc tcctcagtcc taatactggg tgtcagaaat ccgttcgtgg taagtccttc
     2521 aacaggcctg tcctgattat gtcgaagagg ctcaagacga atgctgtcta acaatgcggt
     2581 cgggggaggt cgcactagcc gtatctcctc tttcctctgg gtagtt
//
LOCUS       DATMTI010000637         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_24250, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000637 DATMTI010000000
VERSION     DATMTI010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24250"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VN039_10115"
     CDS             <1..520
                     /locus_tag="VN039_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWV46352.1"
                     /translation="LWSKMPQLTIYLKEVLAQPLTRVPGTAVYLTQFPDLTPPSFVQN
                     VRHNKVLHEQLVFLTTTTARVPIVTGGHHIRIEPLAKGIRRVVAQYGFMETPDITRLL
                     AACRSQGLDLDLDQATFFLSRVNSLATPKPGMALWRERLFIFLSRNSQRASSFFHIPA
                     EQVVEIGVVVEI"
     gene            complement(659..1033)
                     /locus_tag="VN039_10120"
                     /pseudo
     CDS             complement(659..1033)
                     /locus_tag="VN039_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
     gene            complement(1094..1345)
                     /locus_tag="VN039_10125"
     CDS             complement(1094..1345)
                     /locus_tag="VN039_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46353.1"
                     /translation="MRTTLSLDSDVAALLKRAQKLRKASFKTVVNDAMRQGLKDLLEP
                     PARPKKAFRTKGVSLGRCLVGSLDDVAEVLATAEDEAFR"
     gene            1416..1718
                     /locus_tag="VN039_10130"
     CDS             1416..1718
                     /locus_tag="VN039_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46354.1"
                     /translation="MQLEDFPRCSTDSQPTLQRKFRLPYSIPTEQVVEIDVVVEIESV
                     GDRIRSWFDRHGRAVLIRSLDGMVDSTVFVLGLKFSDGAEVNSTCGTNWTASSLSP"
BASE COUNT          427 a          546 c          520 g          417 t
ORIGIN      
        1 cctctggagc aaaatgccgc aactgacgat ctacctgaaa gaggtgctgg cacagccact
       61 cacccgcgtc ccgggtactg cggtttattt gactcagttt ccagacctca cgccgccgtc
      121 cttcgtgcag aatgtgcggc acaataaggt gctccatgaa cagctggtct ttctgaccac
      181 cacgactgcc cgcgtgccga tagtcaccgg cggccatcac atccgcatcg aacctctcgc
      241 aaaaggtatt cgacgagtcg tggcgcaata cgggttcatg gagacaccgg acattacccg
      301 cctcctcgcg gcatgccgga gccaaggatt ggacctggac ctcgatcagg cgacgttttt
      361 cctcagccgc gtgaattccc tggcgacacc aaaacccggc atggcgctgt ggcgagaacg
      421 gctcttcatc ttcctcagcc gcaattcgca gcgcgcgagt tcgttcttcc atattcccgc
      481 cgagcaggtc gtcgagatcg gcgtggtggt ggaaatctag tcactgccat tggcgatcaa
      541 aggttcattc cataacatca ataaggcata acctaacgcg tccgcccatg cggcgtgaat
      601 tcttgtagcg ttgtaccaag caggtcgagt ggagacaggt aggatccgag caagtcagtt
      661 atggtcttcg ttcttgaaga ggatcgttcc agcggaggtt gcggaagcga gcaaaatcac
      721 gatcagtgga gcagagggtc aacccatgct cgatggccaa cgccgccaaa tgtgcatcag
      781 gcacaagatt cgagcgggac aatgtgtcgg tgagtaaaga accgaggacg tcccgatgac
      841 gctctgtggg ctggggaatc cagacgaccg gacaagtcag ccagtcctct acctggttcc
      901 aggctttgga aatcggttgc ggatgttcaa aaatacgggg gttcgacacc agtcgcacga
      961 agctgagcag cgtgggccac ggtaatccta ccggagtggg gccgtttaaa tgtgcgtcga
     1021 gccaagcccg cgcggcttga tgttgggaga gaggtcacat gtgcgtaaat aaggaggttc
     1081 gcatcaacca ggatcatcgg aaggcttcgt cttctgctgt ggccagcact tccgccacat
     1141 cgtccaagct gccgacaaga caacgaccca gtgagacacc tttggtccgg aaggctttct
     1201 tgggccgcgc cggcggctcc aaaagatcct tcagtccttg acgcatcgca tcattaacca
     1261 cggtcttgaa actagcctta cgaagcttct gggctcgttt caagagggcg gctacgtcac
     1321 tatcaaggct cagcgtcgtt ctcatgcatc aaagcatata aagtgtgatg tcatgatgtc
     1381 aacttgaaat ccaaaactgt cctggtctca agccggtgca gttagaggac tttccgcgct
     1441 gctctacaga ttcccagccg actctccagc gcaagttccg tcttccatat tccattccca
     1501 ccgagcaggt cgtcgagatc gacgtggtgg ttgaaatcga gtcggtgggc gatcgcattc
     1561 gatcctggtt cgaccgccac ggccgcgccg tgctgatccg cagcctcgat gggatggtcg
     1621 atagcactgt ctttgtactc ggtctcaaat ttagcgatgg cgccgaggtg aactcgacct
     1681 gcgggacgaa ctggacggcc tcatccttga gcccttagaa gcattgagtc ttcaagcaat
     1741 ttggtattga tcccgacctc catactctca tgtggccgaa cggagccaat ttcgcgcctg
     1801 agtttctaca tgccaatatc cgagttatcg cctcacgtcc ataagcgatg tcatctcacc
     1861 cagcatgttg aaagatcccg gctggcctcg tagcggagca ctcccaaaat
//
LOCUS       DATMTI010000638         1715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_533931, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000638 DATMTI010000000
VERSION     DATMTI010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533931"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="VN039_10135"
     CDS             complement(<1..1519)
                     /locus_tag="VN039_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWV46355.1"
                     /translation="MASLKKKKKDLPLHAARIEDYCLIGDCETAALVSREGSIDWLCW
                     PTFSSGACFAALLGTRDHGFWKVAPDGKIKAIRRRYRERTLIVETTFETSKGEVCVID
                     FMPPRDEHSSVVRIVRGVRGEVAMRMELAIRFDYGRTTSWVTSTKGELRAVAGPDMVV
                     LQTEAPLRGEDMTTVSEFKVKAGESVTFSLTYLSSLAEVPPAAPPAKALAETQRLWTE
                     WTHRNQYNGLYKEAIERSLITLKALSYRPSGGLVAAVTTSLPEKIGGVRNWDYRYCWL
                     RDTSFTLMVLMLAGYEEEAIAWRRWLLRAIAGAPDQIQTIYGIWGERQLLEWEADWLP
                     GYEDSLPVRVGNAAVGQFQLDVFGEVAAALARTPEAEQSIRVPATALQASLVDHLCDV
                     WQQPDEGIWETRDGRKHFTYSKVMAWVAVDRAIKHYEQFDGGGDIKRWRKIRKEIHEE
                     VCEKGFNKKMNTFVQSYGSKELDASCLRIVMVGFLPPEDPRIRGTVEAIERGLMKG"
BASE COUNT          370 a          545 c          412 g          388 t
ORIGIN      
        1 cgcccttcat caggccgcgt tcaattgcct ctacggttcc gcgaatacgt gggtcttcgg
       61 gtggaagaaa cccaaccatg acaattcgca gacaggaggc atcgagctcc ttcgatccgt
      121 aagactgcac gaaggtattc atttttttat tgaatccctt ttcacacact tcctcatgta
      181 tctcttttcg aatcttcctc catcgcttga tatctccgcc gccatcaaat tgttcatagt
      241 gcttaatcgc gcgatcgacg gctacccacg ccatgacctt cgagtatgtg aagtgcttgc
      301 gcccatcgcg cgtctcccat attccttcgt caggctgctg ccaaacgtca cacagatgat
      361 cgaccagcga agcctgtaga gcggtcgcgg gaacgcgaat gctttgctcc gcttcaggag
      421 tgcgtgcaag cgcggctgcg acttcgccaa acacatctaa ctggaactgg ccgaccgcag
      481 cattaccgac ccgcacagga agtgaatctt cataacccgg cagccagtcc gcttcccact
      541 ccagcaactg tcgttctccc catatcccgt agatcgtctg gatctgatcg ggagccccgg
      601 cgatcgcacg caacaaccat cgacgccacg cgatggcctc ttcttcatat cccgccagca
      661 taagcaccat caacgtgaaa gatgtatctc gcagccagca gtagcgatag tcccagttgc
      721 gcacgcctcc aatcttctcc ggcagcgagg tcgtaacagc cgcgacgagt cctcccgagg
      781 gccggtacga caatgccttc agcgtgatca acgaccgctc aatcgcctct ttgtacaggc
      841 cgttgtattg attgcgatgt gtccactccg tccacaacct ctgcgtctcc gctaatgcct
      901 tcgctggcgg agcggctggt ggaacttcgg ccagcgaaga cagatatgtc aggctaaagg
      961 tcaccgattc gcccgccttc actttgaatt cgcttacggt ggtcatgtct tcaccacgca
     1021 acggagcctc cgtctgcaac acgaccatgt cggggcccgc taccgcgcgc aactctccct
     1081 tcgtgctggt gacccacgag gttgtgcggc catagtcgaa gcggatcgca agctccattc
     1141 gcatcgcgac ctcgcctcgc acaccgcgaa cgatccgcac cacgctggaa tgctcgtctc
     1201 gcggaggcat gaagtcgatc acgcacacct ctcccttgga ggtctcaaag gtcgtctcga
     1261 caatgagtgt gcgctctcgg taccgacgtc gaatcgcctt aatctttccg tcaggcgcaa
     1321 ccttccagaa tccatggtcg cgcgtcccaa ggagcgcagc gaaacatgcg cctgatgaga
     1381 aagtgggcca gcacaaccag tcgatcgatc cttcgcggga gaccagcgct gccgtctcgc
     1441 aatctccaat caggcagtaa tcttcaattc ttgccgcgtg aagcggtaga tccttttttt
     1501 tcttcttcaa acttgccatc cgtccctctt tggccataac gctccgtcct cagcatgcct
     1561 cagcaaccgt agacgcctcg agcggcatct ctctagacag tgacttccct gtaaacgata
     1621 ggatgcagag agtcggatat ctgtctaaac gaatcattaa catgcaagat tttggaattg
     1681 agttgaatga aacctgttga aggtcgcaag cgcgt
//
LOCUS       DATMTI010000639         5053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_788509, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000639 DATMTI010000000
VERSION     DATMTI010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788509"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..243)
                     /locus_tag="VN039_10140"
     CDS             complement(40..243)
                     /locus_tag="VN039_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWV46356.1"
                     /translation="MNHPSRLIEIKGPKRAKDLLRELNLLAEAHLVIRGDELVTEDEV
                     LSDKDQIEIRPVISGGSCRPDLR"
     gene            complement(343..1725)
                     /locus_tag="VN039_10145"
     CDS             complement(343..1725)
                     /locus_tag="VN039_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWV46357.1"
                     /translation="MPTRFRLEDFALREVLHRVEHTIRRGGGRVWLVGGSVRDLVLGR
                     QPRDLDLEVVGLPPGQVHALLTEHFSVQFVGKAFEVFKLQGLPVDVSIPSRIVLDDSS
                     LPGLLRQADPGMPIDEALARRDFTINAMAWDPDTMELRDPFNGRGDLDSRILRHASTR
                     FADDPLRVLRGMQLSARFELTVAPETVALCRTLSQEGQPSERLWEEWKKLLLQGRKPS
                     LGLQFLRHCGWLHFYPELAALEGCPQDPIWHPEGDVWIHTLHCLDWFAAERTGHEQDD
                     LAVGLGVLCHDFGKPATTREEYGHVISRGHESEGEDPTRRFLERLTNQHDLIDEVVPL
                     VCCHLRPRALHDANASDGAVRRLAKQVHRIDRLVRVARADHAGRPPKQFDGFPAGDWL
                     LSRAQQLAVDRRAPLPIVMGRHLLELGIQPGPDMGRLLDDCYEAQLDGDFGTLEDGLA
                     YVKSRLSAPC"
     gene            complement(1726..2730)
                     /gene="galT"
                     /locus_tag="VN039_10150"
     CDS             complement(1726..2730)
                     /gene="galT"
                     /locus_tag="VN039_10150"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HWV46358.1"
                     /translation="MPDLRRDPIVGRWVIISTERRGRPHDFVKLQPPRPVSTALCPFC
                     PGQERLTPKEIMAYRPQPSGPNSPNWTVRVVPNKFPALQVEGDMGREGVGLYDRMNGV
                     GAHEVIIETPNHIESLADLPPKKIEDMLWAYRDRMIDLRKDLRFRYILIFKNHGASAG
                     ATLEHSHSQLIALPIVPTNVQEELDGCRKHYQQKERCIYCDILRQDLSDGDRIVAENS
                     EFLCVTPFAPRFPFEMWLLPKRHAAYFEESQKSQFEFLAPILSESLRRMDKVLSRPPY
                     NFILHSSPLHEKTDDYYHWHLEVIPKLTQVAGFEWGTGFYINPVSPEEAATFLREAVI
                     "
     gene            complement(2847..>5053)
                     /locus_tag="VN039_10155"
     CDS             complement(2847..>5053)
                     /locus_tag="VN039_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HWV46359.1"
                     /translation="SMKHVHVCFLWHMHQPYYTDPVAGSASMPWVRLHATKAYYDMAY
                     LLEKFPGVRATFNFTPSLLQQLQEIGTGKIHDLFLEHAQRPAAELTLEETAFLVRHFF
                     SANWATMVRPYPRYHELLVKRGLNILEHDLHRVARQFSTQELLDLQIWHNLAWFGYGT
                     VQQYPRLTALRQKNRGFTEDDKQEVLELQRLAVREILPLYQRLMEQKQIELTTTPFYH
                     PILPLVIDTDITRRARPDLPLPARFHAPDDAAAQLQQAVAFHHQTFGRPPTGLWPSEG
                     SVCPEMLPLIHQARLRWFATDEGILARSLGMCGRPWHRQSALYQPYRIGEAEHALTVL
                     FRDREISDAFGFIYHKTTPESAAEDVLRRLRSIVHDAPQDKLVIAVVLDGENPWEHYH
                     DGGERFLSLLYEAFIHHELDQAGQIVVQASTISDAIASVQPTQHLPCLHSGSWINSDY
                     KIWIGHHEDNRGWDLLGHTRAQLMDLAPSLPSDRAQAAWQELYAAEGSDWFWWYGDDF
                     DTDFKPEFDRLFRTHLRNVWTHMGVPPPDQLNSPICTRTIGSEFESVQQPLALLNPTI
                     DGMVTDFFEWRGAGSINTRPPLGAMWKADGLFTAIRFGWNLDHLVMRFDPDESNTTRQ
                     DLGVDITLQGPRFTFRLTFSLSSPGPETFLLSRRTQAEAWQEIGSYGSINAHAILELT
                     VPWKELCLEQGNTVQMSIIVREHGLEVARYPGHCPAVLTVPGPEFEAGLWRV"
BASE COUNT         1102 a         1348 c         1552 g         1051 t
ORIGIN      
        1 gggagatagg tctggatgct atgcgagatc agtgagttcc taccttaaat cgggccggca
       61 tgacccaccg gaaattaccg ggcgaatttc gatctgatcc ttgtcggaga gcacctcatc
      121 ttcggtcact aattcgtcgc cccgaatcac caggtgtgct tcggccagaa ggttcaattc
      181 gcgcagcagg tctttggccc gcttcggccc cttgatctcg atcaatcgcg atgggtggtt
      241 caattgtacg tgcatgagag gataatacgt gttccatcgg ccaatgggca agggtgttaa
      301 ggcgcaacga gtgagcgcgt gcaaggccgg agaagaagct gatcagcagg gagcggagag
      361 tcgactcttt acataggcga ggccgtcttc aagcgttcca aagtctccat caagttgagc
      421 ctcataacag tcgtcaagga gccgtcccat gtcaggacct ggctgtattc ccaattccaa
      481 taggtgacga cccatgacaa tgggaagagg agcacggcgg tccactgcca gctgttgggc
      541 ccgagacaac agccagtcgc cggcgggaaa tccatcaaac tgtttcggtg gacggcctgc
      601 gtgatcggcc cgtgccacgc gtaccaatcg gtcgatgcga tggacttgct ttgccaatcg
      661 acggactgcg ccgtccgaag cgttggcgtc atggagggca cgaggacgaa gatgacaaca
      721 caccagagga accacctcgt cgatgaggtc gtgctggttg gtcagtcgct caagaaaacg
      781 tctcgtcgga tcttctcctt ccgattcatg tcctcgcgag atgacatgcc catattcctc
      841 tctggtcgtg gccggcttgc cgaagtcgtg acagagtacg cctaagccga cggcaaggtc
      901 gtcctgctca tgtccggttc gctcggcagc gaaccagtcc aggcagtgca gtgtgtgaat
      961 ccagacgtcg ccttccgggt gccagatggg atcttgtggg cagccttcaa gggcggcgag
     1021 ctccggatag aaatggagcc agccgcagtg acgtaagaat tgcaaaccca gcgacggttt
     1081 gcgtccttga aggagcagct tcttccattc ttcccacaga cgctcgctcg gctgaccctc
     1141 ctgggatagt gttcggcaca gagccactgt ctccggcgct acggtgagct caaaacgagc
     1201 agacagctgc atgccacgca agacgcgcag aggatcgtcg gcgaatcggg tcgatgcgtg
     1261 gcgcaatatc cgtgaatcaa gatcgccgcg gccgttgaag ggatcgcgaa gctccatggt
     1321 gtccggatcc caggccatgg cgttgatggt gaagtctcgc cgtgccagcg cctcgtcgat
     1381 cggcatgccg ggatcggctt gtctcagcaa gccggggagg gatgagtcgt caagcacgat
     1441 acgggacgga atggaaacat cgaccggcag accttgcagc ttgaagacct cgaaagcttt
     1501 tccgacgaat tgcacggaga agtgttcggt cagcagtgcg tggacttgac ccggcgggag
     1561 tccaacgact tcgaggtcca gatccctggg ctgtcggccg agcaccaggt cgcgtacaga
     1621 gccgccgacg agccagactc ttccgccccc acgacggatg gtgtgttcaa cgcggtgaag
     1681 aacctccctg agggcgaaat cttcaagacg aaagcgggtc ggcacttaga tcaccgcttc
     1741 gcggagaaac gtcgcggcct cttccggtga caccggattg atgtagaaac cggtgcccca
     1801 ttcgaagccg gcaacttgcg tcagtttggg gatgacctcc agatgccaat ggtagtaatc
     1861 gtcggtcttt tcgtgaaggg gggagctgtg gaggatgaag ttgtacgggg ggcgggacag
     1921 cactttgtcc atgcggcgta acgactccga aaggatgggg gccagaaatt caaactgcga
     1981 tttctgactt tcctcaaaat acgccgcatg ccgcttggga agaagccaca tttcgaacgg
     2041 aaaccgcggc gcgaacggcg tcacacagag gaactccgaa ttctcggcga cgatccgatc
     2101 gccgtccgac agatcctgcc gaaggatgtc gcagtagatg caacgctcct tttgctgata
     2161 atgcttgcgg catccgtcaa gctcctcttg cacgttggtc gggacgatcg gcagagcgat
     2221 gagctgcgag tgactgtgtt ccagtgtggc gccggcagac gcgccgtggt tcttgaaaat
     2281 gagaatgtag cggaaccgaa gatcctttct gagatcgatc atgcggtctc gataggccca
     2341 cagcatgtcc tcgatcttct tgggcgggag atccgcgaga ctttcgatat ggttgggggt
     2401 ttcgatgatg acttcgtggg cgcccacgcc gttcatgcgg tcgtagagtc cgaccccctc
     2461 gcgacccatg tcaccttcga cttgcagcgc gggaaattta ttcggcacga cacgcacggt
     2521 ccagttggga gaattgggtc cgctgggctg tggtcgatag gccatgattt ctttcggcgt
     2581 gagccgttcc tgtcccgggc aaaacggaca gagggccgtc gaaacaggtc ggggcggttg
     2641 gagtttgacg aagtcatgtg ggcggcccct tcgctcggta gaaatgatca cccaacggcc
     2701 gacaatgggg tcacgtctca aatcgggcat gggctccctc tattcgaagt tgcaagtgct
     2761 gagtcccgag tgctcagtgt ctcagagggg gaaaaatttc acaactcaaa actcagcact
     2821 caactctcag cacacagcac ttccgcttag accctccaca gtccggcctc aaattccggc
     2881 cctggtacgg tgaggacggc cggacaatgg ccgggatagc gggccacttc gaggccatgt
     2941 tctcgcacga tgatggacat ctggaccgtg ttcccttgtt ccaggcacaa ctctttccaa
     3001 ggaacggtca attccagaat ggcgtgggca ttgatcgacc cataggagcc gatctcctgc
     3061 caagcctccg cctgagtccg gcgagacaag agaaatgtct ccgggcccgg tgaagaaagc
     3121 gaaaacgtaa gccgaaacgt gaaccgaggc ccttgcaaag tgatgtcgac gccgaggtcc
     3181 tgtcgagttg tgttcgactc gtcggggtcg aatctcatca cgaggtgatc caggttccat
     3241 ccgaatcgga tcgcggtgaa gagtccgtca gctttccaca tggcgccaag cgggggccgg
     3301 gtgttgatgc tccccgcgcc gcgccattcg aaaaagtctg tcaccatgcc gtcgatcgtg
     3361 ggattcaaca aggcgagcgg ctgttggacc gactcgaact cggagccaat ggtcctggta
     3421 cagatcgggc tgttcagttg atcgggaggc gggacgccca tatgcgtcca cacattgcgt
     3481 agatgagttc gaaagagccg gtcgaattcc ggtttgaaat cggtatcaaa atcatcgccg
     3541 taccaccaga accagtcgct gccttctgcg gcgtacagtt cttgccaggc cgcttgcgcg
     3601 cgatcagacg gaagactcgg tgcaagatcc ataagctgtg cgcgggtgtg accgaggaga
     3661 tcccatccgc gattatcttc atggtgtccg atccagatct tgtagtcgga attgatccaa
     3721 gagcctgaat ggagacaggg gagatgctgt gtgggctgaa cggaagcgat cgcatcggaa
     3781 atcgtcgatg cttggaccac gatctgtccg gcttgatcca gttcgtggtg gatgaaggct
     3841 tcgtatagga gggaaaggaa tcgttcgccg ccgtcatgat agtgttccca tgggttttca
     3901 ccgtccaaaa cgacggcgat gacgagttta tcttgcggtg cgtcgtggac gatgctccgc
     3961 aaacgccgca ggacatcctc ggcggcggat tcgggcgttg tcttgtggta gatgaatcca
     4021 aatgcgtcgg agatctcgcg atcgcggaac agtacggtca aagcatgctc ggcttcaccg
     4081 atgcgatagg gttgatagag cgcggactgt cgatgccatg ggcggccgca cattcccaga
     4141 gagcgtgcga ggatgccttc gtccgtggca aaccagcgaa gacgggcttg atggataaga
     4201 ggaagcattt cgggacacac cgatccctct gatggccaca aacctgtcgg aggccggccg
     4261 aatgtttgat gatgaaaagc gacggcctgt tgaagttggg ctgcagcatc atccggggca
     4321 tggaagcgag cgggcagggg gagatccggc ctggcacgcc gggtaatatc ggtgtcgatg
     4381 accagcggca gaatcggatg atagaaggga gtcgtggtca gttcgatttg cttctgctcc
     4441 atgagtcgtt ggtacagggg gaggatttct cgcaccgcca accgttggag ttccagtact
     4501 tcctgcttgt catcctcggt gaagccacga tttttctggc gcagcgcggt cagacgagga
     4561 tattgttgaa cagtgccgta tccgaaccat gcgagattgt gccaaatttg aagatccagc
     4621 agctcttgtg tggaaaactg acgggcgacg cggtgaagat catgctccag tatgttcaat
     4681 ccccgtttca ccaacaactc atggtatcgt ggatagggcc gcaccatcgt cgcccagttg
     4741 gccgaaaaga aatgacggac cagaaaggcc gtttcttcaa gggtcagctc ggccgccggg
     4801 cgttgtgcgt gttccagaaa gagatcgtgg atcttcccgg tgccgatttc ctgcaattgc
     4861 tggagaaggg atggcgtaaa attaaatgtg gcccgaacac cgggaaattt ttccaacaga
     4921 taggccatat cataataggc cttggtcgca tgcaggcgta cccacggcat gctggccgat
     4981 ccggccaccg gatcggtgta atagggttgg tgcatatgcc acagaaaaca tacgtggaca
     5041 tgtttcatcg agc
//
LOCUS       DATMTI010000640         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_108555, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000640 DATMTI010000000
VERSION     DATMTI010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108555"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..>1525
                     /locus_tag="VN039_10160"
     CDS             168..>1525
                     /locus_tag="VN039_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46360.1"
                     /translation="MPSTIPYDPSLVLGSLVHPNKLKVLMQIDEAQRPADAAEDELNS
                     LISLRRSLDMTIQELKDMVIDATDVQAELVEVNKRITKAAIDYAKAKVTAEKTIQPLK
                     ASIGTIDVEIESPIDYNQSAIKPLPLSADSFKMNVQYFAFDKNTQDSLTYASQISAFV
                     SDQVDYLGEDAKAEVSTTINAQTNSQHARHEIVGTLVISITCTHKTASVWAPFIVDVD
                     KAVRAWNTLYPSEMINPTDPANIAQIAKQAYSSKDPCLSLLSGATFGSCFIGMVHVLN
                     VTDTRSSEEMYSLATKVQDQFTVGAWFAQESGGLGVDASFSADTKNLLSAQNITSHCT
                     LVVMGLIPSIKSNNVQMAVKGFADDDGSKSMQSLLKLQNATATEADSVDASAAAARTG
                     GQLVAMKNAQVQGTLSGLADIDNQQNKMIDTNSLIDAMDDYVQQCRTGNMGVPINYYL
                     KP"
BASE COUNT          392 a          386 c          433 g          314 t
ORIGIN      
        1 ggaacgatag ggttctgaat gtgagagctg tcatcagtcg gtggcattgg cggtggacga
       61 cagacatacg ttaggggtgt atgagccgat gatggcgctg aacgatccat gaatggatac
      121 ctgcaactcg aagtattgaa ccggctattg cacaaaggag aagaaccatg ccctctacca
      181 ttccctatga tccatcactc gtgctcggca gtctggtgca tccgaacaag ctaaaagtcc
      241 tgatgcagat cgatgaagca cagcggccgg ccgacgcggc tgaagacgag ttgaattctt
      301 tgatctcgct gcggcgcagt ctggatatga ccattcaaga attgaaagac atggtgatcg
      361 acgccacaga cgtgcaagcg gagcttgtgg aggtgaacaa gcgtatcacc aaggcggcca
      421 tagattatgc gaaggcaaaa gtcactgccg aaaaaacgat ccaaccgctg aaggccagca
      481 ttgggactat tgacgtcgag attgaaagtc cgatcgacta taaccagtcc gcgatcaagc
      541 cactgccgct ttcggcggac tcgttcaaga tgaacgtgca gtacttcgca ttcgacaaga
      601 acacgcagga ttcgttgact tatgccagcc aaatcagcgc tttcgtcagc gaccaggtgg
      661 actaccttgg agaggacgct aaggcggagg tgagcaccac cattaatgcg caaacgaata
      721 gccagcatgc ccggcatgaa atcgtcggga ccttggtgat cagtatcacc tgtacgcata
      781 aaactgcgtc agtctgggct ccgttcatcg tcgatgtgga caaggctgtc cgagcatgga
      841 acaccctgta tccctcggaa atgattaacc cgaccgatcc ggcgaacatc gcgcagattg
      901 ccaagcaggc ttacagttcg aaggatccct gtctgtcgct attgtcgggc gcaaccttcg
      961 gttcttgttt catcggcatg gtgcacgtgc tcaacgtcac cgataccaga tcgagcgagg
     1021 aaatgtatag cttggccacg aaggtacagg atcagttcac ggtcggagcc tggttcgccc
     1081 aggaatcggg cggtctgggt gtggatgcat cgttttccgc cgataccaag aatctgttga
     1141 gcgcacagaa catcacgtcc cattgcacgc tggtcgtgat gggactgatt ccctcaatta
     1201 aatcaaataa cgtgcaaatg gcggtgaagg ggtttgccga tgacgacggc agtaagtcga
     1261 tgcaatcgct cttgaaattg cagaatgcca cggcgacgga ggcggattcg gtggatgcct
     1321 cggctgccgc tgcgcgcaca ggcggtcaat tggttgcgat gaagaacgcc caggtccaag
     1381 gaacgctaag cggcctggcc gacatcgata atcagcagaa caagatgatt gatacaaatt
     1441 cgttgatcga cgccatggac gactacgtgc agcaatgccg gaccggcaac atgggcgtgc
     1501 cgatcaacta ctacttgaag ccgat
//
LOCUS       DATMTI010000641         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_618043, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000641 DATMTI010000000
VERSION     DATMTI010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618043"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VN039_10165"
     CDS             complement(<1..631)
                     /locus_tag="VN039_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV46361.1"
                     /translation="MSTLSRDRLFCRCPLNCGGELQESRITLAEGPLLRCTMCGQLLS
                     SCTRDRYETALKKWDTSSGTTPVARSAVRFQQVTERRLRCALRLRGRLFPPPLRLLDV
                     GCSSGALLAVAAKLGFSPSGVEPASKAAETAQRTGFDVFCGLLHDASFPDAAFDVITA
                     FELIEHLNDPLLLLNECSRILKPEGILIVNTPNASSWTAGVMRECWDGFN"
     gene            complement(761..1564)
                     /locus_tag="VN039_10170"
     CDS             complement(761..1564)
                     /locus_tag="VN039_10170"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWV46362.1"
                     /translation="MIVTTYNRPDALAVVLEGYCSQSDHDFWLVVADDGSKGETAEVV
                     RQFARRAPFPLTHVWHEDRGFRAAAIRNRSVASSDADYVIFTDGDCVPSRHFVRVHKQ
                     LAEPGYFLGSNRVLLSAGLTNRVLHQQQPIHTWSGIDWIQSWFRREVNRVLPLIHLPD
                     APFRKWTPDRWKGIKTCNLSVWRTDLIRVNGLDESYEGWGLEDSDLVIRLLHAGIKHK
                     NARLAATVFHLWHPEQDRVSLPDNQKRLDDLLCSSTVRAEVGLDQYGSV"
     gene            complement(1573..>2055)
                     /locus_tag="VN039_10175"
     CDS             complement(1573..>2055)
                     /locus_tag="VN039_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46363.1"
                     /translation="VPRRNYFMGRWVEHSGWYPNFRQPQLFRKGSMKYVESPVHEGYE
                     LLTAKPVGRLQHAIWQIPFRDFEEVVKKANRYSSLGVLKLADQRVTMGTALFHGIWSF
                     LKHYVAKRGFLDGWAGFVIAFGNFEGTFYRYAKRFEQQEMWSLPKQAPLQRRGDSPSK
                     "
BASE COUNT          477 a          625 c          540 g          413 t
ORIGIN      
        1 gattaaaccc atcccagcat tccctcatga caccggccgt ccaacttgaa gcattcggtg
       61 tattaacaat gaggataccc tccggcttca agatcctaga gcattcgttc aacagcagga
      121 gcgggtcgtt caggtgttca atgagctcga atgccgtgat aacatcaaac gccgcgtcag
      181 gaaaggaggc gtcgtggagc agcccgcaaa atacatcgaa tccggtacgc tgagccgtct
      241 cagcagcttt actcgctggt tccacgccgc tcggggagaa tcccaacttt gcggcaacag
      301 caagcaacgc tccgctggag caacccacat cgagcaaacg gagcggtgga ggaaacaatc
      361 gaccccgcag acggagagcg caacgcaacc gtcgctcagt cacctgctga aagcgaacag
      421 cagaccgggc caccggtgtt gtaccacttg aggtgtccca tttcttcagc gctgtctcat
      481 agcggtcacg agtgcaactg ctgaggagtt gaccgcacat cgtacatcga agcagcggtc
      541 cttcggccaa ggtaatcctg ctctcctgca actcaccgcc gcagttgagg gggcaacggc
      601 aaaacaagcg atcccgtgaa agtgtcgaca cgggagttgg ctcgtcattc ccctcgtttc
      661 cggacatagg cgttccgcct ccgatgtttc tcccaggcga tcggtgatca accgcttcgc
      721 ctacttgcac gactgccgtg tcggaatcgc cccgtccgta ttacacagag ccgtattgat
      781 cgagcccgac ttcggcgcga acggtcgacg aacacagcag atcatccagg cgtttttgat
      841 tgtcgggcag actcacgcga tcctgctcgg gatgccataa atgaaacacc gtcgcggcca
      901 agcgggcatt tttgtgcttg atcccggcat ggagtagtcg aatgaccaaa tcagaatcct
      961 ccagtcccca tccctcgtag gattcgtcaa gtccgttcac acggatcaag tcggtccgcc
     1021 acacggagag attgcaagtc ttgatgcctt tccaacgatc cggcgtccat tttcgaaagg
     1081 gagcatcggg cagatggatc agcggaagga cccgattgac ttcgcgccga aaccaggact
     1141 ggatccaatc gattccgctc cacgtatgaa tcggctgctg ctgatgcagg acgcggttcg
     1201 tcagcccggc cgagagcaac acacgattcg atccgaggaa gtaacctggt tcggccaatt
     1261 gcttatggac acgcacgaaa tggcgcgagg gaacacagtc accgtctgtg aaaatcacat
     1321 agtcggcgtc cgacgacgca acggagcggt tgcggatcgc ggcggctcga aacccccggt
     1381 cctcgtgcca gacatgcgtc agcggaaacg gcgcgcgccg tgcaaattgc ctcacgacct
     1441 cagccgtctc ccctttcgac ccgtcatctg caaccacgag ccagaaatcg tgatcgcttt
     1501 ggctgcagta accttccagt acgaccgcca gcgcatcagg gcgattgtat gtcgtcacga
     1561 tcaccgctgt tttcatttcg acggtgagtc gccacggcgt tgaagcggcg cctgtttggg
     1621 gagcgaccac atctcttgct gttcgaaccg cttggcatac cgataaaacg ttccctcgaa
     1681 atttccgaag gcaatgacaa acccggccca cccgtccagg aaccctcgct tcgccacata
     1741 atgtttgagg aacgaccaga tgccgtgaaa taaagcggtg cccatcgtca ctctttgatc
     1801 ggcaagcttc agcaccccga gcgacgaata ccggttggcc tttttgacga cctcttcaaa
     1861 gtccctaaat ggaatctgcc agatcgcatg ctgcaaccgg ccgaccggtt tggcagtcag
     1921 taattcgtac ccttcatgga ccggggattc cacatatttc atagaccctt ttcgaaacaa
     1981 ttgaggctgg cggaaattcg gataccaacc ggaatgttcg acccagcgcc ccatgaagta
     2041 attccgcctc ggcac
//
LOCUS       DATMTI010000642         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_788598, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000642 DATMTI010000000
VERSION     DATMTI010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788598"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..435
                     /locus_tag="VN039_10180"
     CDS             70..435
                     /locus_tag="VN039_10180"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWV46364.1"
                     /translation="MMARHPSVVLAILFGSMAKDRARNDSDLDIAVATSTPLTARTHI
                     AIIEDLALAFGRPVDLIDLDRTHNPLLQQILIKRCRVLCHDRTRYAELLLRMVYEEAD
                     FMPYYRRILSYRRQAWIET"
     gene            420..596
                     /locus_tag="VN039_10185"
     CDS             420..596
                     /locus_tag="VN039_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46365.1"
                     /translation="MDRDLVETKLESLRRCVQRIAEKTPSSVDQLIRDPDAQDIIALN
                     LHAQSSFQLMSPCI"
     gene            587..838
                     /locus_tag="VN039_10190"
     CDS             587..838
                     /locus_tag="VN039_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HWV46366.1"
                     /translation="MHLIAETDAAPPSTMAENFEILRKLQIIDPVLAERLTKAVGFRN
                     IAVHSYQAINWNVVYQICRHHLDDFRQFTKAVVHHLQQV"
     gene            851..1036
                     /locus_tag="VN039_10195"
     CDS             851..1036
                     /locus_tag="VN039_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46367.1"
                     /translation="MRSEYKRADFTKLERGKFYSAVAAGTSVALLEPAIAKAFPTSKA
                     VNEALAGLLVLTEKTHA"
     gene            1130..1381
                     /locus_tag="VN039_10200"
     CDS             1130..1381
                     /locus_tag="VN039_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46368.1"
                     /translation="MKRKSEVSSDDLRPEYIFDYSKAVRGKYYRRLVKEGANVVVLEP
                     DVAKAFHDSKAVNDALRSLLKVARLSTRSSRTARKHTAA"
     gene            1841..>2053
                     /locus_tag="VN039_10205"
     CDS             1841..>2053
                     /locus_tag="VN039_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV46369.1"
                     /translation="MIVTIAISCTASFDLVPEELNDLSKFNFLFGSNGTGKTTVSRVI
                     SDEAKFPSCKVTWKAGTKLQPVVYNHD"
BASE COUNT          515 a          571 c          506 g          461 t
ORIGIN      
        1 gtgatactca aaagcatgga gctacagtag ccacctctta ctcctgatct tgatcgaaag
       61 atcgagtaca taatggcccg ccatccatcg gtggtattgg ctattctgtt cgggtcgatg
      121 gccaaggatc gcgcgcgcaa cgacagtgac ctcgacatcg cggtcgcgac atccacgccg
      181 cttaccgctc ggactcacat cgccatcatt gaagaccttg cgttggcctt tggccgtcct
      241 gtggatctca tagaccttga cagaactcac aacccattgc ttcagcagat cctgatcaag
      301 aggtgcagag tcctgtgtca cgatcggacg cgttacgccg aacttcttct ccgtatggtc
      361 tatgaagaag cagacttcat gccctattac cgccgcattt tgtcgtacag acgacaggca
      421 tggatcgaga cctagtcgaa actaaactgg aatcgctccg tcgttgcgtc cagcgaatcg
      481 ctgagaagac tccctcttct gtggatcagc tcattcgcga ccccgacgcg caggacatca
      541 tcgcactgaa tctccacgcg cagtccagct ttcagttgat gtcgccgtgc atctgattgc
      601 cgagactgat gcggccccac cctctacgat ggctgagaac ttcgagattc tcaggaaact
      661 tcaaatcatc gatcctgtct tagcagaacg tctgacaaaa gcggttggtt tcaggaacat
      721 cgccgtccac agctatcaag cgatcaactg gaatgtcgtc tatcaaatct gccgtcatca
      781 tttggacgac tttcggcaat tcaccaaggc ggttgtccat catcttcagc aggtctgaaa
      841 aaaagacgaa atgcgttccg aatacaaacg ggcggacttt acaaaactgg aaagaggaaa
      901 attttattcg gcagtagccg caggaacttc agtcgccttg ttggaaccag ctattgccaa
      961 ggcctttccc acctcaaaag cagtcaatga agcacttgcc ggcttactcg tattgactga
     1021 aaaaacacac gcataaccag acgctcaacc cggacgcgca aaaaggcaac gcgcgccact
     1081 tagtggtgcg ttaactctcc cgtaggtacg cgacacgaga ggaaacgaca tgaaacgtaa
     1141 gagcgaagtc tccagcgacg atctccgccc cgaatacatc ttcgactatt cgaaggccgt
     1201 tcgggggaaa tattatcggc gcctcgtaaa ggaaggggcg aatgtcgttg tccttgagcc
     1261 tgatgtcgcc aaagccttcc acgattcgaa agcggtgaat gatgcattgc gctccctcct
     1321 gaaggtggct cgcctatcta cccgctcaag ccggacggca cgaaaacaca ccgccgctta
     1381 gagggggcac gttaaacgct tgaacaggag aatcccttta aaagcaatgg ggatacatct
     1441 tattctgtgc atgctctcaa gctaggcaag cccatggcgc gatcgttgcg catctcattt
     1501 gctacgaggc tgaatccttc ctgatggaag gtgatttcga gactgcacat tcgcagccac
     1561 tcgcgaagta ataatccgaa tcggtaccgc cgatgtatgc gcattcggcc tcactgggac
     1621 gactgcgaac agctcttcgc ccagctcggc tatgcgcgca ggtcagcatc gtcgcccggt
     1681 agtttcctac aatgcacatc ggtgcgccgc taaggagagc gttgacaagt gctgggacat
     1741 gagcaagaat tccgcgccac ttttcggttc aactgcgagt gctacgccgg tcagtatggc
     1801 aagcggcttg gaatgagatg cattcgagag actgcgtgaa atgattgtaa ccattgccat
     1861 ctcttgcaca gcctctttcg acctcgttcc cgaagaactc aacgacttgt ccaagttcaa
     1921 ttttctattt ggctcaaacg gcacaggaaa aaccactgtc agtcgtgtga tctcggatga
     1981 agccaagttc ccctcgtgta aagtgacatg gaaagcggga accaaacttc agccggtggt
     2041 ttacaaccac gat
//
LOCUS       DATMTI010000643         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_321878, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000643 DATMTI010000000
VERSION     DATMTI010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321878"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VN039_10210"
     CDS             <1..479
                     /locus_tag="VN039_10210"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWV46370.1"
                     /translation="CLADLLQRHRRDELHIDIPVIISNHDTCASWADLFKIPFAVYPV
                     TKETKPQQEQQVLAVLKKHRVELVVMARYMQILSADFLAQVGCPVINIHHSFLPAFIG
                     ANPYRQAYDRGVKIIGATAHYATQDLDEGPIIEQDVIRVGHRDTVEDLVWCSTSGY"
     gene            complement(528..2012)
                     /locus_tag="VN039_10215"
     CDS             complement(528..2012)
                     /locus_tag="VN039_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HWV46371.1"
                     /translation="MSSQSYRTIGSAIGIGLLSGALVWGSHSLTASYASNAPSPTAMP
                     VAVASPANGFTEVAKQVTPAVVNITTVMTEKVSEGFSVPDELRDRMEEFFGKPFGPRA
                     EGPSGPFEHRGPRKGQGSGVIISSDGYILTNNHVIAKAREVHVTLPDKREFKGKIVGT
                     DPKTDLAVVKVDAQDLPAVLWGDASQLQVGEYVLAVGNPFGLNSTVTLGIVSAVGRGH
                     MGITQYEDFIQTDAAINPGNSGGALVNTKGELVGINTAIFSQTGGYQGVGFAVSTTMA
                     RPIYENLVSIGKVVRGYLGIGIQDLNQDLAKSFNIKNSKGALISDVKEEGPAEQAGLK
                     QGDVIIEYHGTPVEDGVALQRLVTRTSVGTTVPLKVIRDGHEREMTIRIGEQPDETKV
                     AKVERAETDSALSGLAVEDLDLATSKELGLNVKRGVVVTRVAPDSGAEQAGLMPGDII
                     REINRQPIRSVKDFEKLSSNVKKGGTVLILVNRRGNSLFLSAKV"
     gene            complement(2152..3555)
                     /locus_tag="VN039_10220"
     CDS             complement(2152..3555)
                     /locus_tag="VN039_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV46372.1"
                     /translation="MPLRVRLTLWYGTALALVLMIFSVVLYAMTARNLRDAVDQSLEE
                     TATTAVRSLEERGFLPLINEEELLSQFPELTRIDKFFQIFSPSGTISIRSPNIKQHEV
                     PLSRTALDRAFAGQSIFESTKYPNEPPLRLISMPIMYRGNLLYIVQVGTSMESVGETL
                     HRFLILLVVAIPIALTVSLAGGWFLAGRALRPVDKITVAAQRIAAGDLGQRLSMSAAH
                     DEIGRLAATFNNMIGRLDVSFRQIRQFTSDASHELRTPLTVMKGETDLVLRRPRALED
                     YKSVLESNLEEIDRMTRIVDELLFLSRADMGEVKMESLPVVMESLVEDIHRQATLLGQ
                     DRNIEVVLGTVMPVVVQGDDLRLRELLLNLVENAMKYSYPGGKVEISLLNDGREARLS
                     VTDHGIGIAPADHKRIFHRFFRTDIARAHTKKGTGLGLAICAWIADLHKGRVEVKSDL
                     GQGSTFTVVLPLAHSAA"
     gene            complement(3740..4411)
                     /locus_tag="VN039_10225"
     CDS             complement(3740..4411)
                     /locus_tag="VN039_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV46373.1"
                     /translation="MRVLVIEDETKVGCFIKRALEEESYAVDLCEDGAKGLEMALAIN
                     YDLLVVDVMLPSMSGLDVLKNIRRERIHTPVLILSAQSQIDQRVKGLDAGADDYLTKP
                     FAIDELLARVRALLRRGASESPGILQVDDLILNPATRDVTRGGQRIDLTLKEYALLEY
                     LMRHTGRVLTRPMISEHVWNQDFDTFTNVIDVYVNYLRNKIDRGRTKKLIHTIRGSGY
                     MLKAD"
     gene            complement(4512..5102)
                     /locus_tag="VN039_10230"
     CDS             complement(4512..5102)
                     /locus_tag="VN039_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46374.1"
                     /translation="MVKAIIIVLVLVTVVGIGYAGYAIFKNQTLVTKFLAPHIPTSQP
                     GVVLTDPRSKPFQPDVRLTPEGIALLLDALDEAVRRKDVDGVLRHITHDATITIHLKQ
                     GPQQQTAMLTREDYRKTLTMSFAFPSANDFTRTNTSVSLASDERSAKVSFKATETLRQ
                     ANREFKTEGEETLVFSIRDGKPMITSLEQTFPGDST"
BASE COUNT         1172 a         1475 c         1410 g         1188 t
ORIGIN      
        1 actgtctggc cgatctgctc cagcgacacc gccgagacga actgcacatc gatattcccg
       61 tcatcatctc gaatcacgac acctgcgcga gctgggctga tctcttcaag attccctttg
      121 ccgtctatcc cgtaacgaag gagaccaagc cccagcaaga gcaacaagtc ttggctgtcc
      181 tgaaaaagca ccgagtggaa ctggtggtca tggctcgcta catgcagatc ctcagcgcgg
      241 actttcttgc tcaggtcggc tgcccggtca tcaacatcca ccattctttc ctccctgcct
      301 ttatcggagc caatccctat cggcaggcct acgaccgcgg cgtcaaaatc atcggagcga
      361 cggcccacta cgcgacccaa gatttggacg aagggccgat catcgagcaa gacgtgattc
      421 gtgtgggaca ccgtgatacg gtggaggatc tcgtgtggtg ttcgactagc ggctattgaa
      481 aaagggagcg ggtcggatga ccggctccct ttttttcttt tgcgaaatta caccttcgcc
      541 gataggaaca gcgaattccc gcgccgattg accaagatca acacagtgcc tccctttttc
      601 acattcgagg agagtttctc aaaatccttt acggacctga tcggttgcct attgatctct
      661 cggataatgt cgcccggcat gaggccggcc tgctcagctc cgctgtccgg agcaaccctt
      721 gtcaccacca cgcctcgctt cacattaagg ccgagttctt tggaggtagc cagatctaag
      781 tcttcgactg cgaggccgga caaggcactg tcggtttcgg ctctttccac cttggcgact
      841 ttcgtttcgt caggctgctc accaattctg atcgtcatct cacgttcatg accgtcgcga
      901 atgactttca acggcacggt ggtaccgacc gaggtcctgg ttaccaaccg ctgcaacgcg
      961 accccatcct ccacgggagt gccgtgatac tcgataatga cgtctccctg cttcaggccg
     1021 gcctgctcgg ccggaccctc ttccttgaca tcgctgatga gggcaccctt cgaattcttg
     1081 atgttgaagg acttcgccag atcctgattc aggtcctgga tgccgatacc aaggtagcct
     1141 cggacaacct tgccgatact gacaagattc tcgtagatcg gtcttgccat cgtcgttgat
     1201 acggcaaaac caacgccttg atacccgccg gtctgagaga aaattgccgt attaataccg
     1261 acgagttcac ccttggtatt caccagcgcg ccgcctgaat tgccgggatt gatggcggca
     1321 tcagtctgaa tgaaatcctc gtactgagtg attcccatat ggcctcggcc aaccgcgctg
     1381 acaatcccga gggtcactgt ggagttcaag ccaaacggat tgccgaccgc caacacatac
     1441 tcaccgactt gcagttgtga agcatcgccc cacagtacgg caggaagatc ctgtgcgtcg
     1501 actttgacga ccgcgagatc ggttttcggg tcggtgccga caatcttccc cttgaattcg
     1561 cgcttgtccg gaagcgtgac atggacctca cgtgccttgg caattacatg attgttggtc
     1621 aaaatatatc catcggaaga aatgatgacg cccgaccctt gtcctttccg aggccctcga
     1681 tgttcaaaag ggccggacgg tccctccgcg cgaggcccga atggttttcc gaagaattct
     1741 tccattcggt ctcgtagctc atccggaaca gaaaatccct ctgaaacctt ctccgtcatc
     1801 acggtcgtga tgttgacgac cgccggagtg acttgcttcg cgacctccgt aaagccgttt
     1861 gccggagaag cgacggccac aggcatggcc gtcggtgacg gagcattgga cgcgtaggac
     1921 gcggtgagtg agtggcttcc ccaaaccagg gcgccgctca ggagaccgat cccgatcgct
     1981 gaaccgatgg tacgatacga ttgcgatgac attatttatt tcctccttgt gaagctgttg
     2041 atcgaataat cgacagagaa cgagttcatg accgcaagga tgctttccgg tttcgattac
     2101 aaaaactaac aaccggtaat gagagggaga ttaatcgggg attaaacttc gttaagcagc
     2161 ggaatgagcg agcggtagca caaccgtgaa ggttgatccc tggccgagat cgcttttgac
     2221 ttctacccgg cctttgtgca aatccgcgat ccaggcacag atggcgaggc cgagacccgt
     2281 ccccttcttt gtgtgggcac gggctatatc ggtacggaaa aaacggtgga aaatccgctt
     2341 gtggtccgcc ggggcgatgc cgattccatg gtccgtgacc gacagcctgg cctctcggcc
     2401 gtcattcaac agcgagatct cgacctttcc tcccggatac gaatacttca tcgcattctc
     2461 aacgagattg agcagcaact cgcgaagccg gaggtcgtcg ccttgcacaa ccaccggcat
     2521 aaccgttccc agtacaacct cgatatttcg gtcctgcccg agcaacgtgg cttgacgatg
     2581 aatatcttcg acgagagact ccatcacaac gggcaacgat tccattttga cttctcccat
     2641 gtcggcacgg gagagaaaca acagttcgtc gacgattcgg gtcattcgat cgatctcttc
     2701 caaattgctc tcaagcaccg acttatagtc ttcgagcgcg cgcggtcgcc gcagaaccag
     2761 gtccgtttcg cccttcatca ccgtgagagg ggtccgcaac tcatgcgacg cgtcgctggt
     2821 gaattgacgg atttgacgga acgagacatc cagtcggccg atcatgttgt tgaaggtggc
     2881 ggccaggcga ccgatttcgt cgtgagcggc cgacatgctg agacgctggc cgagatctcc
     2941 cgcggcaatg cgctgcgcgg caacggtaat cttatcgaca ggacgtaacg ctcgccctgc
     3001 caagaaccat ccccccgcca gagaaacagt cagggcgatc gggatcgcca ccacaagcag
     3061 aatcagaaaa cgatggaggg tctctccgac cgattccatc gaggtgccca cttgcacgat
     3121 atacaagaga ttccccctat acatgatcgg catggagatc agccgcaacg gaggctcatt
     3181 aggatacttg gtcgactcaa aaatactctg tccggcgaac gctctatcga gcgcggtccg
     3241 gctgagcgga acttcatgct gtttaatgtt tggggaacgg atcgatatgg tgcccgaggg
     3301 gctgaagatc tggaagaact tatcgatgcg cgtcagttcg ggaaattggg agagcagttc
     3361 ctcttcattg atcaaaggaa gaaacccgcg ctcttcgagc gaacgcacag ccgtcgtcgc
     3421 cgtttcttcc agggattgat cgacggcgtc gcgcaaattt cgcgcggtca tggcatacag
     3481 aaccacagaa aaaatcatca agaccagggc gagtgcggtc ccgtaccaga gggtcagccg
     3541 aacacgtagc ggcatagatc agctcctgat cgcaggtggc gacatgatac cctcgcagag
     3601 gcttcttgtc ggttgaacgc atccggagcc gaagcatgag agacggtttc ttcgcatcgt
     3661 gctagcccat gcaaagccgc atgactgttt gtcgcagcgg tgcatcggcg gttgcagtag
     3721 aagccttcat gaatgtgggc taatcggcct tcagcatata cccgctaccg cgaatggtat
     3781 ggatcaattt cttggttcgg cctcgatcga ttttattccg aagatagttc acatagacat
     3841 cgatgacgtt ggtgaacgta tcgaagtctt gattccacac gtgttcggag atcatgggcc
     3901 gagtgagtac gcggccggta tggcgcatca gatattcgag taatgcatac tctttcagcg
     3961 tcaaatcgat gcgttgccct ccccgggtga cgtcgcgggt cgccggattg agaatcaggt
     4021 cgtccacttg aaggattccg gggctttcgg atgccccacg acgcaacaag gcccgaacgc
     4081 gcgccaggag ttcatcgatc gcaaacggtt tagtcaggta atcgtcggca ccggcatcca
     4141 gtccttttac ccgctggtcg atctgagatt gagccgagag gatcaacacc ggtgtgtgga
     4201 tccgttcccg gcggatattc ttcaacacat ccaaaccgga catggacggc aacatcacgt
     4261 cgacaaccag gagatcatag ttgatcgcca gggccatctc taaccctttg gctccgtcct
     4321 cacagagatc gacagcgtag ctctcttctt ccagcgctcg cttgataaaa cagccgactt
     4381 tggtttcatc ttctatgaca agcacccgca tatacactcc aacacatgtt gattagcggc
     4441 gtgattatac cagaccgatg aatgtgaatc ttgtagcgta cagatacttt cgatcgctcg
     4501 taaaacggcg gctaggtcga atcgccgggg aaggtctgtt ccagagaggt aatcatgggc
     4561 ttgccgtcgc ggatgctgaa gaccaacgtt tcttcgcctt ccgtcttgaa ttctcgattg
     4621 gcttgtcgca gggtctctgt cgccttgaag gacactttcg cgcttcgctc atccgaggcc
     4681 aacgagacgg acgtattcgt acgagtgaag tcgttggcgc tgggaaaggc gaaactcatc
     4741 gtgagggtct tccggtagtc ttcccgcgtc agcatggccg tctgttgttg cgggccttgt
     4801 ttcagatgaa tggtgatggt cgcgtcgtgg gtgatgtggc gcaggacgcc gtcgacatcc
     4861 tttcgtcgaa cagcctcatc gagtgcatcg agaagcaggg cgatcccttc aggagtcaga
     4921 cggacatcgg gctggaaggg cttcgatcgc ggatctgtga gaaccacgcc gggctgactt
     4981 gtcggtatat gaggagccag aaacttggtt accagagtct ggtttttgaa tatcgcgtat
     5041 cccgcgtatc cgatgcccac cacggtaacg agaacaagca cgatgatgat agcttttacc
     5101 atggagatct ctgcggtatt ccctctctgt tcttctatcg aggcccggct tcgtgacaac
     5161 ctaggtcaac tctccggact gaggtatgag ctaacaatag cgtgaattat gcgaaaagca
     5221 atcaaagccg gccgctctgc aagac
//
LOCUS       DATMTI010000644         8706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_702412, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000644 DATMTI010000000
VERSION     DATMTI010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702412"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..501
                     /gene="nuoE"
                     /locus_tag="VN039_10235"
     CDS             37..501
                     /gene="nuoE"
                     /locus_tag="VN039_10235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HWV46375.1"
                     /translation="MLTDIERRELEDALKQYPDKRSGTIDALLIVQRRRGWISDDSLL
                     DIAQFLEMTSEDVDSVATFYNLIFRKPVGRHVAFLCDSISCWIRGCEEIREQLKKHYD
                     IDLGQTTHDGRLTVLPIACLGHCERAPAMMIDQDVYGEVTPEKVESIVEKYK"
     gene            576..1880
                     /locus_tag="VN039_10240"
     CDS             576..1880
                     /locus_tag="VN039_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HWV46376.1"
                     /translation="MERPLTQYIHPDGTPRLLREYVQNGGYRSLQRLAAGMVPKDVQR
                     LVAEAGLRGRGGGGFPTGSKWSFVPMGPDAPKPKYIVANADEMEPGTFKDRILMEGDP
                     HGLVEGMIMAGYALDAEVGYIFLRGEYHRSAQRLTQAIAEAYAEGYLGKPLPGTTFRF
                     DLHLHSSAGRYICGEETALINALEGKRATPRAKPPFPQTVGLWGQPTIVQNVETLYNL
                     PHIVNHGSAWYHSLSRTQDGGTKIYGVSGRVKHPGWWELPLGTTAREVLEQHAGGMLH
                     GVRFRGVLPGGISTAFLIEEHLDLPLDFSSFPAQVGRLGTGTMIVLDDHTCPVGFVLN
                     LERFFARESCGWCTPCREGLPWVARLLQSFEEGRATNDDLALLDMHTKRLAPGHTFCG
                     LAPGAMAPLQSALHYFHDDFERHISLGRCPWSNKPAQLKVLV"
     gene            1885..4644
                     /gene="nuoG"
                     /locus_tag="VN039_10245"
     CDS             1885..4644
                     /gene="nuoG"
                     /locus_tag="VN039_10245"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWV46377.1"
                     /translation="MATIIVDNRSYTVNERQNLLAACLGVGLNVPYFCWHPAMGSVGA
                     CRQCAVKQFKDDRDRSGRLVMACMTPASHGTRISVDDPEAKAFRASVIEWLMVNHPHD
                     CPVCDEGGECHLQDMTVMTGHVYRRYRFPKRTHRNQSLGPFIRHEMNRCIQCYRCVRF
                     YRDYAGGRDLNVFGAHDALYFGREQDGTLENEFSGNLVEVCPTGVFTDKTLFQHYTRK
                     WDLQSAQSICSHCSLGCNTTVGERYGIVRRIMNRFNSQVNGYFLCDRGRFGYEYVNSD
                     KRIKRVLVRPDLDRTKPAKTMGAHEVIAVAAERLRHARGIIGIGSPRASLEANVALRS
                     LVGPQQFFLGIDEREHQLLSTILTILRTGPVPSASIHDTEQSDAVFVLGEDLLNNAPR
                     LALALLQSVRQQPMERVDELKIPRWDEAAVRNAVQNRRGPLFMAGHRRTWLHEYATDS
                     YFAAPDDIARLGFSVAGTIGQAAPSVPDLTDDVRDLAQRIADALTHAQRPLIIAGTGS
                     GSQSTIEAAANVAWALHQHGKQPRLNFVVPESNSIGLALLGGGRLQEAFDSVSREQAD
                     TVIVLENDLYRRADQAKVDAFLKTAQTVMVIDSLEHRTAGHADVLLPAATGPESDGTL
                     VNQEGRAQRFYRVFVPEGDVTDSWRWVRDLARAQSQQTDSFTGVATWSNFDDAVSATA
                     ATAPELARIGEVAPHASFRVMGQKIPRQPDRYSGRTSMLSHLTVHEPPPPPDPDTPLG
                     FTMEGHRGPLPSPLIPQFWAPGWNSIQAVNKYQDEVGGPLREEMPGLRLIEPIATTVP
                     NWFTAIPHVFQPISHQWLLLPLTALFGSEELSNQSPAIVERISEPFLSLHPSDGARLS
                     LDDRSMIELALQGTTYRLAVHLDPSTPVGTAGISLGPNVFDVRVPVWIDLFKAIKAGQ
                     RRRVA"
     gene            4641..5597
                     /gene="nuoH"
                     /locus_tag="VN039_10250"
     CDS             4641..5597
                     /gene="nuoH"
                     /locus_tag="VN039_10250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWV46378.1"
                     /translation="MIDWNHTALTIGVMLGGVLTLAGMLIWVERRLLGIWQERYGPNR
                     LGPGGVLQSLADMIKIFTKEDWIPPFADKAVFVLTPAIVVITALMSFAVVPIAPSFVI
                     ADLNMGVMFFLAMSSLAAYSVIIGGWASNSKFAFLGSLRAIAQLLSYEVFMGLSLMGA
                     VLLAGSFNLSDIVEAQRHQWFVVPQFLGFLGFFMAGLAEAHRTPFDMPEAEAELVAGY
                     HSEYSGMKFGMFFVGEYLSVILLSAMTVILFFGGWHGPWLPPVVWFVLKMAFFIVLII
                     LIRATWPRFRFDQLLSFGWKVVMPLALINLLATGGVVLWKAG"
     gene            5676..6218
                     /gene="nuoI"
                     /locus_tag="VN039_10255"
     CDS             5676..6218
                     /gene="nuoI"
                     /locus_tag="VN039_10255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWV46379.1"
                     /translation="MTNIWTYTHDLVLGLWIVFKRTFTKPVTVQYPEERPYLPPRWRG
                     RIVLTRDPDGEERCVACYLCAVACPVDCIALQATEDPTAHERRYPEFFRINFSRCIYC
                     GHCEEACPTNAIQLIPDFEQSEYARRNLVYEKEDLLISGTGKYHDYNFYRVAGVRTRD
                     KDKGQGENELPPVDVRSLMP"
     gene            6309..6806
                     /gene="nuoJ"
                     /locus_tag="VN039_10260"
     CDS             6309..6806
                     /gene="nuoJ"
                     /locus_tag="VN039_10260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWV46380.1"
                     /translation="MEVLFYIAAAVTFLATLRVITHVHAVHALLYLVVALIALALIFY
                     LLGAQFAAALEIIIYGGAIMVLFIFVVMLLGPLAVDQERTWLTPGAWVGPSVLALVLL
                     GEVGYLIAVGDHMPITAAETRQKGVSIALYGPYIIGVELASMLLLPGLIGAYHLGRRV
                     PKESR"
     gene            6806..7105
                     /gene="nuoK"
                     /locus_tag="VN039_10265"
     CDS             6806..7105
                     /gene="nuoK"
                     /locus_tag="VN039_10265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWV46381.1"
                     /translation="MLTTPALVLAIMLFGLGLIGLLSRRNILYMLLSLEIMLNAASLA
                     FIAGGARWGQVDGEIMFLFILTLAAAEVSVALGIVLQLSYRFHTLDADAFSEMKG"
     gene            7247..>8706
                     /gene="nuoL"
                     /locus_tag="VN039_10270"
     CDS             7247..>8706
                     /gene="nuoL"
                     /locus_tag="VN039_10270"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWV46382.1"
                     /translation="MLKLLWLIPALPLAGFLVLALCGGRLSRWQVAWVGCGSVGMAAV
                     TTALVGVDFFRAFPDRTSYQQTLWNWIDTSGMTVGVSWYLDPLSFLMVAVITGIGFLI
                     HLYSAEYMSGTGEAGVSAPAGGEGEDHHGYPRFFAYMNLFVAAMLTLVMADNLLLLYL
                     GWEGMGLCSYLLIGFWYREPEYGTAAQKAFIVTRIGDTAFAIGLFILFTQLKSLSIQQ
                     VQTLAVDAWPVGSNVAVVVAALLLIGAVGKSAQLPLQVWLPDAMAGPTPVSALIHAAT
                     MVTAGVYLIARMHHLFALAPLVQETVAVIGLLTLLLAACSALVQRDIKRVLAYSTMSQ
                     IGYMFLALGVGAWSAALFHFLTHSCFKALLFLAAGSVIHSLHHEQDIFRMGGLRRQLP
                     WTFWTFLIGAAAMSGVPLITAGFYSKDWILWSVWSSPLSHQWIWFGALLGTLLTGLYS
                     FRLIFRVFFGEAHTAASGEPGFAMRIPLVVLAFFSLT"
BASE COUNT         1719 a         2408 c         2556 g         2023 t
ORIGIN      
        1 agacagacga gatagaccag acagacgaga tagacaatgc tgaccgacat cgaacgccgt
       61 gagcttgaag acgcattgaa acagtaccca gataagcgat cgggaacaat cgatgcgctg
      121 ttgatcgtac agcgtcgacg cgggtggatt tcagatgact cacttcttga tattgcacaa
      181 tttctggaga tgacgagcga ggatgtagat agtgtcgcaa ccttctacaa cctcatcttt
      241 cgcaaacccg tcggtcggca tgttgccttc ctgtgcgaca gtatcagttg ttggatcagg
      301 ggatgcgaag agattcgtga acagctcaaa aaacactatg acatcgatct gggacagacg
      361 acgcacgacg ggcgcttgac cgtcttgccg atcgcatgct tggggcattg tgaacgggcg
      421 ccagccatga tgatcgatca ggatgtgtac ggcgaggtga cgcccgaaaa agttgaatcc
      481 atcgtagaga aatacaaata gggaagataa tggcaaaagg ttgaaggtga ggaggtttga
      541 ggcctcctac ctctaacctc aaaccggctt caaacatgga acgtccactc actcagtaca
      601 ttcatccgga tggtaccccg cggttgctgc gtgaatatgt ccagaacggt gggtatcgca
      661 gcctccaaag gctcgctgct gggatggttc ccaaggatgt gcaacggcta gttgccgaag
      721 caggactgcg gggcagaggt ggaggcgggt tcccgacagg gagcaaatgg agcttcgtgc
      781 cgatggggcc cgatgctcca aagccgaagt atatcgtcgc gaatgccgat gaaatggaac
      841 cgggtacctt taaggatcgg atcctcatgg aaggggaccc gcatggcctc gtcgaaggca
      901 tgatcatggc cgggtatgcc ctggatgcag aggtcggcta cattttcttg cgcggggaat
      961 atcatcggtc tgcacagcgt ctgacccaag ccattgccga ggcctatgcg gaaggatatc
     1021 tcggcaagcc cctccctggg acgacctttc gttttgacct ccatctccat tccagcgcgg
     1081 ggcggtacat ctgtggtgag gaaacggcgc tgatcaatgc gttggaaggc aaacgagcga
     1141 ctcctcgcgc aaaacctcca tttccacaga cggtcggttt gtggggacag ccgaccatcg
     1201 tgcagaacgt cgagaccttg tacaaccttc ctcatatcgt gaaccatggc tccgcttggt
     1261 atcacagtct aagccgtact caggacggcg gcacaaagat ctatggagtc agcgggcgcg
     1321 tcaaacatcc tggatggtgg gaactgccgc tcggtacgac ggcccgtgag gtattggagc
     1381 agcacgccgg tggaatgttg cacggcgttc gctttcgagg cgtcttgcct ggcggtatat
     1441 ccacggcatt cctgattgag gagcacctcg atcttcccct ggacttttct tcgtttccgg
     1501 cccaggtcgg tcgtctcggg accggcacta tgatcgtgct cgacgatcac acctgtccgg
     1561 tggggtttgt gctgaatctt gaacggttct ttgcccgtga atcctgcgga tggtgtacgc
     1621 cctgccggga agggttgccg tgggtcgctc gactcctcca gtcgttcgag gaaggacgcg
     1681 ccaccaacga cgatttggcg ctcctggaca tgcatacgaa gcggcttgca cccggtcata
     1741 cattctgtgg tctcgcacct ggtgcgatgg cccccttgca atcggcctta cactactttc
     1801 acgacgactt cgaacgacat atcagcttgg ggcgctgtcc ctggagtaac aagcccgcgc
     1861 agctgaaggt cctggtctaa ttgtatggcg acgatcattg tcgataatcg atcctatacc
     1921 gtcaatgaac ggcagaattt gctggccgcc tgtctcggag tcgggctgaa cgtgccctac
     1981 ttttgctggc acccggcgat gggatcggtc ggcgcgtgcc gccaatgcgc cgtcaaacag
     2041 ttcaaagacg accgagatcg gagcggtcga ctggtgatgg cctgcatgac ccccgcttcc
     2101 catggcacgc gtatttcggt cgacgatcct gaagcgaagg cctttcgcgc ctcggtcatt
     2161 gaatggttga tggtcaatca tccgcacgac tgcccggtct gtgacgaagg tggggagtgt
     2221 catttgcaag acatgacggt catgactggg catgtctacc gtcgctaccg gtttcctaaa
     2281 cggactcatc gcaatcagtc tctcggccca ttcattcggc atgagatgaa ccgatgtatc
     2341 caatgctacc gctgcgtacg attctaccga gactacgcgg gcgggcgaga cctcaatgtc
     2401 ttcggcgccc atgacgccct ctatttcggc agggaacagg acgggacgtt ggagaatgaa
     2461 ttcagcggaa atcttgtgga agtctgtccg acgggtgtct tcaccgacaa aacgctgttc
     2521 caacactaca caaggaagtg ggatcttcaa tcagcccaat cgatctgctc ccattgcagt
     2581 cttggctgca ataccactgt cggtgaacgg tacggcatcg tgcgtcggat catgaaccgg
     2641 tttaacagcc aggtaaacgg ctactttctc tgtgatcggg ggcggttcgg ctacgaatat
     2701 gtcaacagcg acaagcggat caagcgagtc cttgtgcggc cggacctcga tcgtaccaag
     2761 ccggcaaaga cgatgggagc gcatgaggtg atcgcggttg cagcagaacg tttacgacat
     2821 gcccgcggaa tcattggcat cggttcgcct cgtgcgtcgc tagaagccaa tgtggcgctt
     2881 cgctcattgg tcggaccaca gcaatttttt ctagggattg acgagcgcga acatcagctt
     2941 ctctccacca ttctcaccat tcttcggacc ggaccggtgc cctccgcgtc cattcacgat
     3001 acagaacaat cagacgccgt cttcgtcctg ggcgaagacc tcttgaacaa tgcgccccga
     3061 ttagcgctgg cccttctgca atcagtccga caacagccga tggaacgagt cgacgagttg
     3121 aagattcccc gatgggatga ggccgcagtg cgcaatgcgg tgcagaatag gcgagggcct
     3181 ctcttcatgg ccgggcatcg gcggacttgg ttgcatgagt acgcaacaga cagctatttc
     3241 gctgcaccag atgatattgc ccggttggga ttctccgttg ctggaacaat cggacaggct
     3301 gcaccatctg ttccagatct caccgacgac gtccgcgacc tcgctcagcg cattgccgac
     3361 gcgctgaccc acgcgcagcg gccgctgatc attgccggaa cgggcagtgg ttctcaatcg
     3421 acgatcgaag cggcagccaa tgtggcgtgg gccttacatc agcatgggaa gcagccacgt
     3481 ctcaactttg tggttccgga gagcaatagc attggcctcg cgttgctggg tggtggacgt
     3541 ttgcaggagg cgttcgactc ggtcagtcga gaacaggctg acacggtgat cgtgctggag
     3601 aatgatctgt atcgacgcgc ggaccaggcg aaggtcgatg cctttctgaa gaccgcacaa
     3661 accgtgatgg tgatcgattc cctcgagcat cgcaccgcgg ggcatgccga cgtgctcctc
     3721 ccggcggcca caggtccgga atctgatggg acgcttgtca atcaagaagg gcgagcccag
     3781 cggttttacc gagtgtttgt cccggagggt gacgtcaccg acagttggcg ttgggtcaga
     3841 gacctcgccc gtgcacaatc acagcagaca gacagcttca ccggagtggc aacctggagc
     3901 aattttgacg atgcagtctc cgcgacagca gcgacggctc ctgagctggc acggattgga
     3961 gaggtggccc cgcatgcgag ttttcgtgtg atgggccaaa aaatcccacg tcagccggac
     4021 cgctatagtg ggcgcacatc gatgctgtcc catctgaccg ttcacgagcc tccacctcct
     4081 cccgatccgg acacaccact gggatttacc atggagggac accgcggacc actcccttcg
     4141 ccgctgatac cccaattctg ggctcctgga tggaattcca tccaggctgt gaacaaatat
     4201 caggatgagg tcggcggacc cctgcgtgag gaaatgccag gattgcggct catcgagccg
     4261 atcgcgacga cggtaccaaa ttggtttacg gccattccgc atgtatttca gccgatctcc
     4321 catcaatggt tgctgttgcc actgactgct ctgtttggca gcgaggagct cagtaatcaa
     4381 tctccagcga ttgttgaacg gatttcagaa ccgttcctgt ccttgcatcc gtcagatggg
     4441 gcgcggctga gccttgacga ccgaagcatg attgaattgg cgttgcaggg gacgacctat
     4501 cgccttgccg tacacctcga tccttcaacc ccggtaggga ctgcaggcat ctctcttgga
     4561 cctaacgtct ttgacgtcag ggtgccggtt tggatcgatc tgttcaaggc gatcaaggcc
     4621 gggcaaaggc ggagggtcgc gtgatcgatt ggaaccatac cgcactgacg atcggagtca
     4681 tgctcggtgg cgtgctgacg ctggctggga tgctgatttg ggtcgaacgt cggctgctgg
     4741 gaatctggca ggagcggtac ggccccaacc gtctcgggcc tggaggagtc ctgcaatccc
     4801 ttgcggatat gatcaagatc ttcacgaaag aagactggat tccacccttt gcggataaag
     4861 cggtcttcgt gctcacaccg gccatcgtgg tgatcaccgc cctcatgtca ttcgcggtcg
     4921 tgccgattgc tcccagtttc gtgatcgccg acctcaacat gggcgtcatg ttctttttgg
     4981 ccatgtcgag tctggcggcc tacagcgtca tcatcggagg gtgggcctcc aacagcaaat
     5041 ttgcctttct cggcagcctt cgggcgatcg cgcagctgtt gagctatgaa gtctttatgg
     5101 ggctgtccct catgggcgcg gtgcttttgg ctgggtcgtt caacctgagc gacattgtgg
     5161 aagctcaacg gcatcaatgg tttgtcgttc cgcaatttct tggttttctc ggcttcttta
     5221 tggcggggct ggccgaggct catcgcacgc cgttcgatat gcctgaggcg gaagccgagc
     5281 tggtcgccgg ctaccactcg gaatacagcg ggatgaaatt cgggatgttt ttcgtcggtg
     5341 aatatctcag tgtcatcttg ctctcggcca tgaccgtgat tctcttcttc ggcggctggc
     5401 atggaccttg gttgccgccg gtggtgtggt tcgtcctgaa aatggccttc ttcatcgttc
     5461 tgattatcct tattcgagcg acctggcccc ggtttcgctt tgaccagctc ttgtcgttcg
     5521 gttggaaggt cgtgatgccg ctcgccctca ttaatcttct ggcgacggga ggagtggtgc
     5581 tgtggaaagc aggatgaagg aattcacagc ataaccgtga gcttggactg tcgcatgtgt
     5641 gtgaggggca ccaaaccatc agcacggtgc tacgcatgac aaacatctgg acctataccc
     5701 acgatctggt cctggggtta tggatcgtct tcaaacggac gtttacgaag ccggtcaccg
     5761 ttcagtatcc agaggaacga ccctatttgc ctccacgctg gcgtggacga atcgtcctga
     5821 cccgagatcc ggacggagaa gagcggtgcg tggcctgcta cctctgtgcg gtggcttgcc
     5881 ccgttgattg catcgcgctt caggccaccg aagacccgac cgctcatgag cgccgctacc
     5941 cggaattctt tcggatcaat ttctcgcgct gcatctattg tggacattgt gaagaggctt
     6001 gtccgaccaa tgccattcag ctcatcccgg attttgaaca aagtgaatat gcgcgccgaa
     6061 atctggtcta cgaaaaagaa gacctcttaa tcagcggaac cggcaagtat cacgactata
     6121 acttttatcg agtggccggc gtgagaactc gcgacaaaga caaggggcaa ggtgaaaacg
     6181 aactcccgcc ggtggatgtg aggagtttga tgccctagga caggaacgtg tagcaaatgg
     6241 cttatagcac aaaataccta gccaccctct tccttgttac tgccataagc actacgccat
     6301 aagctcttat ggaagttctt ttctacatcg ctgccgccgt aaccttcctg gcgactctgc
     6361 gcgtcatcac gcatgtgcat gccgtccacg cgctgcttta cctggtcgtg gcgctgatcg
     6421 cgctggctct gattttctac ttgctcggcg cgcagtttgc cgcagcgctt gaaatcatca
     6481 tttacggcgg tgccattatg gtgctcttca ttttcgtcgt gatgctcctg gggccgctcg
     6541 ccgttgacca ggaacggact tggctcacgc ccggcgcatg ggtgggaccc agtgtgctgg
     6601 ccctggtgct actgggtgag gtgggatacc tcattgcggt cggggaccat atgccgatca
     6661 ccgccgcaga aaccagacag aaaggggtct ccatcgccct ctatggaccc tacatcattg
     6721 gggtggaatt ggcttccatg ctgttgcttc cggggctgat cggcgcctac cacttggggc
     6781 gtcgtgtacc gaaggagtct cgttgatgtt gaccacgcct gcgctggtgt tggccatcat
     6841 gctgtttggc ctggggttaa tcggtctctt gagtcgacgg aacattctgt acatgctgct
     6901 gtcgcttgag atcatgctca atgccgcaag tctcgcgttt attgccggag gcgctcgttg
     6961 gggacaagta gacggcgaaa tcatgttctt gttcattctt accctggcgg cggctgaagt
     7021 ctccgtggca ctgggtatcg tgctccaact gtcgtaccga ttccatacat tggacgctga
     7081 cgcattcagc gagatgaaag gataaccacg tgaacgatca gtcgaaggac aaagggtttc
     7141 tggtgtcgag tttcgggttt catggcgtgc tttatccgac tcgaaacaag aaactcgcga
     7201 caggaaacgt tttctagcgg ggtttcacga acgacgagag acgagaatgc tgaaactact
     7261 ttggcttatc ccagcacttc ccctcgccgg ctttctggtg ttggcactgt gtggagggcg
     7321 tttatctcgg tggcaggtcg cctgggtcgg ctgcggctcg gtgggcatgg cagccgtcac
     7381 caccgcgctc gtcggcgtcg attttttccg agcctttccg gatcgtacat cctaccagca
     7441 gaccctgtgg aattggatcg acacgtccgg catgacggtc ggagtctcct ggtacttgga
     7501 cccgctgtca ttcctgatgg tggcggtcat caccggtatc ggctttttga tccatctgta
     7561 ctcagcagaa tacatgtcag gcaccgggga agcgggcgtc tcggcgccgg cgggggggga
     7621 gggtgaggac catcatgggt acccgcggtt cttcgcctat atgaatctgt ttgtggcagc
     7681 gatgctgacg ctggtgatgg ccgataatct tcttctgctc tatctcggct gggaggggat
     7741 ggggctctgc agctaccttt tgatcggctt ttggtaccgc gaaccggaat acggcaccgc
     7801 cgcgcagaag gcctttatcg tgactcgcat cggagatacg gcgtttgcca tcggcctctt
     7861 tatcctcttc actcaactga agagtttatc gatccaacaa gtccagacgc tagccgttga
     7921 cgcatggccg gtcggatcga acgtagccgt tgtcgtggcc gcgctgctgt tgatcggggc
     7981 ggtcgggaag tcggcgcagc tgcctctgca agtctggttg ccggatgcga tggctggtcc
     8041 caccccggtc agcgcgctga tccatgcagc cacgatggtg acggcggggg tctaccttat
     8101 cgcgcgcatg catcatctct ttgcccttgc gccgctggtg caggagaccg tcgccgtgat
     8161 cggcctgctg accttgctgc tcgcagcatg cagtgcgttg gtgcaacgtg acattaagcg
     8221 ggtgctcgcc tattcgacca tgagccagat cggctatatg tttcttgcgc tcggtgtcgg
     8281 cgcctggtct gccgcgctct ttcactttct gacccattcc tgcttcaagg ccctgctctt
     8341 cttagcagct ggatccgtca tccacagtct ccaccatgag caggacattt tccgcatggg
     8401 aggccttcga cggcagctgc cgtggacatt ctggacattt ctgatcgggg ccgcggcaat
     8461 gtccggtgtt cccttgatca cagccgggtt ttacagcaag gactggatct tgtggtccgt
     8521 ctggtcctca cccttgagcc atcagtggat ctggttcggc gcgttactcg ggacactact
     8581 caccggactc tacagcttcc ggttgatctt tcgggtcttt tttggagagg cccatacggc
     8641 ggcgagtgga gagccgggct ttgcgatgcg aattcccctt gtcgtgctgg cgttcttctc
     8701 cctcac
//
LOCUS       DATMTI010000645         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_831688, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000645 DATMTI010000000
VERSION     DATMTI010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831688"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="VN039_10275"
     CDS             complement(<1..1977)
                     /locus_tag="VN039_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWV46383.1"
                     /translation="MSIPGAPDLRPQERKLPPLRTNLQFNRGAPTPDGVPTWTIVDPI
                     RNRYFQIEWSVYQMIKHWSAGTIEKLHAVMARETTCRTTVGDVEDFVKFLYTNNLTEQ
                     SAGGKHTDYQIQAQAGEQNWMMWLLHHYLFVKIPLVHPHNVLRATLPYVAPFYTVAAG
                     WMFGMMGLIGLILVSRQWDAFTSTFLYFFNWRGAILYSLSLGAVKIVHELGHAYTATR
                     FGCRVPTMGIAFIVMMPVLYSDVSDSYRLTSKRKRLLIAAGGVIAELGLASVALFAWG
                     FLPDGTVRSITFIVATTSVIMSLAVNLNPFMRFDGYYLLADSLSIPNLQDRAFAFGQW
                     QLRGLLFGCFSSPPETTSVALRRTLIAYAWAIWLYRLVLFTGIAVMVYQYFFKALGII
                     LFLVEIVWFIAMPIAREITSWWTNRAFYAASSRAWGTAVILGILLSLVFIPIPTSVSA
                     PAVLQAASYATIYAPMPGRISQVLVRNGQRVMAGEQIAVLENPSLKKEVRLAEIKAAM
                     WTYRLGRQAGHDQDRAQRQVIAESLKAELAELAGLESKQANLILKAPMAGMIRDRADS
                     LIPGRWIDQKLPVFYLIDAGHAVIEGLIPVDELAYIDVGQSARFIPADLTSPSVEAQV
                     AKIAEVDEQDVAISYFASIFGGDMAVRKDDKGRLK"
     gene            complement(1981..3348)
                     /locus_tag="VN039_10280"
     CDS             complement(1981..3348)
                     /locus_tag="VN039_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWV46384.1"
                     /translation="MATTAEPTAQQFPTLIHLAALTHLEGQIRAAKTVQELQFLSVNE
                     TRRLVPYDQAFLLSPSGPGTNAYCVLNASSVAVVDRDASMIVWLEQAVQALRQSVNTS
                     EQPMVVTEELLPDSLRSGWREFVKGHVFWCPLQHPDETVLGAWWFERDFAWQENDVAI
                     VHRLASSYAYAWKALSKKQQGWSRKIGRPAWWLLPIAALGLFCLPIRISVVAPVKVMA
                     KDPVVVSAPMDGVIADMLVPPNQMVQPGASLFRYEDTNLRNQFRVAEKQLAVAHAEHG
                     QAVQAGFSDPQRKADVPLKAVEADLRQTELDYAKEMLDQVEVKASKPGLLLYSDKSDW
                     IGRPVTVGERIMEIADPKQIELRIDLPVADAIVLKEGAEAVVFLNALPLESFAATVVH
                     TSYHAEVLPGDMLAYRVTARLAQPDSRVRIGWQGTAKIYGEQGPLAYLLLRRPLTAIR
                     QWVGW"
     gene            complement(3351..4127)
                     /locus_tag="VN039_10285"
     CDS             complement(3351..4127)
                     /locus_tag="VN039_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV46385.1"
                     /translation="MRHRWLDSVLVLFTVMAVGVPATGWCKGDRLVHATRPESGVARG
                     IVKAAAQAVLYAQVQGRVSQLPYKEGQRFEKGYTLVQLDCDKYRAELAVAIAEHEAKD
                     KVYKNNIELGKLNAVSKLDLETSDAEAKKALAAIRVAEVNVKGCQIAAPFSGRVVSIM
                     INEHENVFPNDKLISLLDDSSLEIELVLPSSSLSWLRRKSSFTFVVDETRRSYPARVK
                     EIGASIDPASQTIKVTGAFEKLPPEILAGMSGSAQFPEQP"
     gene            complement(4105..>4796)
                     /locus_tag="VN039_10290"
     CDS             complement(4105..>4796)
                     /locus_tag="VN039_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV46386.1"
                     /translation="QKMEEQALLLRPELRAIDYKKRINAKEAKAVFLELFPSLKVSFG
                     GYYNSNSLLFYQNWLTYAAQVSWNLLSVFRTPAKLKAIEAHGQMLDAQSLALSLSILT
                     EVHVGAVQFLRAKEEYQDAWNYQRAQAAIVEHTNNLWLTQRTNDLTLIRERVNDVAAD
                     VRLDAARSGLETAYATLMASLGEEAVPTSMGEQNVAQLADSIRQHWESSGFTMSEAER
                     RSEAHATPVAR"
BASE COUNT         1069 a         1393 c         1288 g         1046 t
ORIGIN      
        1 cttaagtcgg cccttatcgt ccttccgcac cgccatatcg cccccaaaaa ttgatgcgaa
       61 gtaggatatg gctacatctt gctcgtcgac ttcggctatc ttcgccacct gagcttcaac
      121 ggatggactg gtcaggtctg cagggatgaa tcgcgccgat tgcccaacat cgatataggc
      181 gagctcatcg acggggatga gaccttcgat cacagcatga ccggcatcga tgagatagaa
      241 aacgggaagc ttctgatcga tccatcggcc agggatgagc gaatctgcac gatctcgtat
      301 catcccggcc atgggggcct tgagaatcaa gttcgcctgt ttggattcga gtccggccaa
      361 ttctgccaat tcggccttca acgactcagc gatcacttgc cgttgcgcac ggtcctgatc
      421 atgaccagcc tggcggccaa ggcgataggt ccacatcgcc gctttgattt ctgccaaccg
      481 cacttctttc ttcagcgatg gattctccaa taccgcgatc tgttcgccgg ccatgaccct
      541 ttgcccgttc cggaccaata cctggctgat acgtccaggc atcggcgcat agatcgtggc
      601 gtaagaggcg gcctgcagaa ccgcgggagc cgagacgctc gtaggaatcg gaataaacac
      661 aagcgagagg aggatgccaa ggatgacggc cgtgccccag gctctcgatg aagccgcata
      721 gaaggcacgg ttggtccacc agctggtgat ctcccgagcg atgggcatgg cgatgaacca
      781 gacgatctcg accagaaaca gaatgatgcc cagtgctttg aagaaatact gatagaccat
      841 tacagcgatg ccggtgaaaa gaaccaggcg atagagccag attgcccacg cataggcgat
      901 gagggtccgg cgcagagcga ctgaggtcgt ctcggggggc gaggagaagc atccgaacag
      961 cagcccgcgc agttgccatt gtccgaacgc gaaggcccga tcctgaagat ttggaatgct
     1021 caggctatct gcgagcagat aatagccgtc gaatcgcatg aacgggttca aattcactgc
     1081 gaggctcata atcacactag tcgtcgccac gataaaggta atactccgga cggtgccgtc
     1141 cggcagaaac ccccaggcga acaatgcgac cgatgcgagc cctaactctg caatcacacc
     1201 gcccgccgca attaagagac gtttccgctt cgacgtcaac cggtaggaat cggacacgtc
     1261 ggaatagagg accggcatca tgacgatgaa ggcaatgccc atcgtcggaa ctcgacagcc
     1321 gaatcgcgtg gcggtgtagg cgtgccccaa ctcatggaca atcttcactg caccaaggga
     1381 caagctatag aggattgccc cgcgccaatt gaaaaagtag agaaaggtcg aggtgaaggc
     1441 atcccattgg cggctgacga ggatcagtcc gatcaacccc atcatgccga acatccatcc
     1501 ggcggccacc gtgtagaagg gcgcaacgta tgggagcgtt gccctcagga cgttgtgtgg
     1561 atgcacgagt ggtatcttga cgaacaaata gtgatggagc agccacatca tccaattctg
     1621 ctcaccggct tgggcctgga tctgataatc cgtgtgcttg ccgcccgcag actgctcggt
     1681 caaattgttc gtatagagaa acttcacgaa gtcttccacg tccccgacag tcgtccgaca
     1741 ggtcgtctca cgtgccatca ccgcatggag tttttcaatc gttccggcac tccagtgttt
     1801 gatcatctga tagaccgacc attcgatctg gaaataccgg ttgcgaatgg gatcgacaat
     1861 ggtccaagtc ggcacgccgt ctggcgtcgg ggcgcctcga ttgaattgga ggttcgtgcg
     1921 tagcggcggg agcttgcgtt cctgtggcct aagatctggt gctcccggaa tggacatgat
     1981 ctaccagccc acccattgtc ggatcgctgt caatggacgc cggagcaaca gatacgcgag
     2041 gggtccttgc tcgccataga tttttgccgt gccttgccaa ccgatgcgga cacgcgaatc
     2101 cggttgtgcc agccgcgccg tcacacggta tgccaacatg tctcccggaa gaacctctgc
     2161 atgataactc gtatggacca cggtcgccgc aaaggactcc agtggaaggg cattgagaaa
     2221 gacaaccgcc tcggcccctt ccttgagcac aatggcgtcg gctaccggca agtcgatacg
     2281 cagctcgatc tgtttcgggt ccgcaatttc catgatgcgt tcaccgacgg tcaccggtcg
     2341 accgatccaa tcagacttgt ctgaatagag gagcaaaccg ggtttcgacg ccttgacttc
     2401 gacctgatcg agcatttctt tcgcatagtc gagctcggtc tgacggagat ctgcttcgac
     2461 tgctttcaag gggacatcag ccttgcgttg cggatcgctg aaaccggctt ggaccgcctg
     2521 accatgctca gcgtgggcga ccgccaattg cttctccgcg acacggaatt gattgcgcag
     2581 gttggtgtct tcataccgga acaacgaggc acccggttgc accatttggt tcggcggcac
     2641 gagcatatcc gcgatgacgc cgtccatggg tgcgctgacc accaccggat ccttcgccat
     2701 gactttgacc ggggccacga cggaaatccg gatcggcaaa cagaacaggc ccagcgcggc
     2761 aatcggcagg agccaccagg ccggtctccc gatctttctg gaccagccct gctgcttttt
     2821 cgacaaagcc ttccaagcat acgcataact gctggccagc cgatggacaa tcgccacatc
     2881 gttttcctgc caggcgaaat cccgttcgaa ccaccaagcc ccgagcaccg tttcatccgg
     2941 atgttgcagc gggcaccaaa agacgtgccc cttcacaaac tcccgccagc cgcttcgaag
     3001 cgagtcaggc agcagctctt ccgtgacaac catcggctgt tcactggtgt tcacagattg
     3061 acgcagcgcc tgaaccgcct gctcgagcca cacgatcatt gacgcatcac gatcaacgac
     3121 ggcaacgctc gaggcgttga gcacgcaata cgcattcgtc cctggacctg aggggctcag
     3181 cagaaaggcc tgatcatagg gaaccagtcg cctggtttcg ttgaccgaga gaaactgcag
     3241 ctcttggact gtcttggccg cacggatttg cccttccaga tgggtgaggg ccgccagatg
     3301 aatcaaggtc ggaaattgtt gtgcggtcgg ctctgctgtc gtggccatgt ctaaggttgc
     3361 tccggaaatt gggctgaccc actcatgccg gccaagattt ccggcggcaa tttctcgaaa
     3421 gcccctgtta ctttgatcgt ctggctcgca ggatcaatcg aagcgccgat ttctttgacc
     3481 cttgccggat aacttcttcg agtttcgtcc acgacgaatg taaatgacga cttgcgtcgg
     3541 agccatgaca gcgaagaaga tggcaacacg agctcgatct caaggctgct gtcgtccaac
     3601 agactgatca atttgtcgtt ggggaatacg ttctcatgct cattgatcat gatgctgacc
     3661 acacgccccg aaaatggagc ggcaatttgg catcctttta cgttgacttc ggcgactcga
     3721 atagcggcca atgccttctt ggcttccgca tcggacgttt ctaggtcaag tttactcacg
     3781 gcattgagtt taccgagctc aatgttattc ttataaacct tgtcttttgc ctcatgttca
     3841 gcgatggcga ccgctaattc tgcacgatac ttgtcacagt cgagttggac cagcgtgtac
     3901 cccttttcga agcgctgccc ttccttgtat gggagctgac tgacacggcc ttggacctgg
     3961 gcatagagga ccgcctgtgc cgccgccttc acgattccac gagcaacgcc ggattccggc
     4021 cttgtggcat gaaccagtcg atctcccttg caccaacctg tcgcagggac acccaccgcc
     4081 atgacggtga aaagcaccag cacgctatcg agccaccggt gtcgcatggg cttccgatct
     4141 cctttccgct tccgacatcg tgaaaccact ggattcccag tgctgcctga tcgaatcggc
     4201 aagttgggcc acgttttgtt cgcccatgct ggtaggaacc gcttcctccc ccagagaggc
     4261 catcaaggtg gcataggccg tttccagccc tgatcgtgcg gcatccaatc gcacatccgc
     4321 cgcaacatca ttgactcgtt ctcggatcaa cgtgagatca ttcgttcttt gtgttagcca
     4381 taagttattg gtgtgctcaa cgattgctgc ctgtgcacgc tgataattcc atgcgtcctg
     4441 atattcctcc ttggcacgaa ggaactgaac ggcaccgaca tgcacctccg tcagaatgga
     4501 caagcttaac gccaggcttt gagcatccaa catttgtcca tgcgcttcga tcgctttgag
     4561 tttcgccggt gttcgaaata ccgacagcag gttccaactc acctgggcag cataggtcag
     4621 ccagttctga tagaagagta aactgttgct gttatagtac ccgccgaatg agactttgag
     4681 actggggaag agctcaagga atacggcctt tgcttccttg gcattaattc gcttcttata
     4741 gtcaatggct cgcagctcag gtctgagcag taacgcctgt tcttccatct tctgac
//
LOCUS       DATMTI010000646         6334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_24774, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000646 DATMTI010000000
VERSION     DATMTI010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24774"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1187
                     /locus_tag="VN039_10295"
     CDS             192..1187
                     /locus_tag="VN039_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWV46387.1"
                     /translation="MSKERIGVGLIGVGRHGARYAQHLVQDLPMASLRAVCRRHPEQG
                     FHMPGAESVTVYGDAQSLIADPTVDVVVVVTPPIYSFEICGLAAHARKPLLIEKPLAT
                     TATDARMMVAQAREAGVPLMTAQTLRFDHTIQHMKMLQPSIGHSQQLDFVFHIEIRKT
                     VPDHADGYGKRGAVLEIGIHMLDLVRFLTGEEIQNVQCTMDRHPPSAPEMRASVHLTT
                     TGGTACRIDIARVLRSRAGRAIWIGSQGRVEADWMHPRIRCVDDAGVQTMNADPPPSQ
                     TVLQTLTAFLQAVKNNSPMPITGEDGCRAVEIAEACYRSAQAGGKPITLPLQPQP"
     gene            complement(1208..1444)
                     /locus_tag="VN039_10300"
     CDS             complement(1208..1444)
                     /locus_tag="VN039_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWV46388.1"
                     /translation="MATRAYILIKVKAGKTKDVVGTLKRIPGVEQAHSCFGRPDIFVF
                     ISVQDERALSDVVITKIHAIDGVEETDTHIVAES"
     gene            complement(1517..2032)
                     /locus_tag="VN039_10305"
     CDS             complement(1517..2032)
                     /locus_tag="VN039_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46389.1"
                     /translation="MLTADQTPSLSVQQPKRSSPHVGSAMAVLATLEQAQVLPPEGSR
                     EADRLIQSVIQFQSAFAKGTDRPVQDFAQRALTDKYGEEATPVLERFRASGWTAELLE
                     ALADADQRTSAEELERLATGFRQFNLSVDDFKRFMQLVRDGRSALAARGQDFEGVFAH
                     HRKAMPGASGH"
     gene            2313..3842
                     /locus_tag="VN039_10310"
     CDS             2313..3842
                     /locus_tag="VN039_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWV46390.1"
                     /translation="MLAKVRSAALVGLDAHLVDVEVDISGGLPQFSVVGLPDAIVKES
                     RDRVRSALKNTGFHFPAKRITVNLAPAGIKKEGSGLDLAIAVGILVAEEVIPQSRLDH
                     RVLVGELSLDGRVKPITGALSFALACRVGYDLLLPADNGAEAAWVDGVSAYPLHSLPE
                     AVEFLNGTQAIVPSRSNHDVLGAARTAEDEDYSDVRGQDHAKRALEVAASGGHNVLMV
                     GPPGSGKTMLARRLPSILPLLESEEAIETTRIHSVAGQLAPDRPLLAVRPFRAPHHSI
                     SDAGLVGGGTIPKPGEVSLAHNGVLFLDESPEFKRGVLEGLRQPLEDGQMILTRASGT
                     LKYPARFMLIAAMNPCPCGYYGDRARPCICTGMQIRRYRAKLSGPLLDRLDIHLDVPP
                     VPVRELRTELPAAETSAAIRSRVVAARERQRRRYRHDGIYTNAQLKPRMVKRYCGLDQ
                     PAQELLEHAMARLRLSARAHGRILRVARTIADLADSDKIETVHIAEAIQYRSFDRNPD
                     M"
     gene            3850..4062
                     /locus_tag="VN039_10315"
     CDS             3850..4062
                     /locus_tag="VN039_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46391.1"
                     /translation="MASVKVFFWWFVVGATMALAVILVQGGIREVMQAQGSVWELKLV
                     ELLTTIMGGGLLGGCIALILDRIKKS"
     gene            4220..5206
                     /locus_tag="VN039_10320"
     CDS             4220..5206
                     /locus_tag="VN039_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWV46392.1"
                     /translation="MPRNDQAVRQLIILKKLESSRHGLTLEQLADSFGPSTTRHPRTL
                     RRDLDAIESAGYPLLTERVDGRTRWKFLEGARQAPALRFSPTELMALALSRRLIAPLE
                     GTALHASLVSALSKVSAALPPEGLALVQQLEGTFSVGLGPHKRYKHHREIVERVTQAI
                     AEKRRVQMRYDSASRRRITRREVDPYRLWYASGGLYLIGYCHLRKEPRMFAVERIKSV
                     TPTELPYQIPLHFDFDAFVEDSLTVMRGPRINVELEFDKATAAWAKDRVWHPSQELKR
                     LQGGKLRITLSVADSRELVGWILSFGGGVKVLRPDALRRAVKEEARRILESQ"
     gene            5315..5737
                     /locus_tag="VN039_10325"
     CDS             5315..5737
                     /locus_tag="VN039_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46393.1"
                     /translation="MSQRYVKLDVIDASQKEAIVKWRLLIAIMLLIPTTASQAEMGFD
                     PRYERDYNIFNPVTKYQSDNPLNPVNKYDPDNAFNPVNQYDPGNPTNPINQYNPNNPF
                     NPVNRYHPDNPLNPVNKFNPSVPFAPLDGRSSRRTGAW"
     gene            complement(5764..>6334)
                     /locus_tag="VN039_10330"
     CDS             complement(5764..>6334)
                     /locus_tag="VN039_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46394.1"
                     /translation="SPASPDSLADYLHTIITALPESTSYCLVGALAVNAWGRLRATKD
                     IDLLVLADLPIRTEVIDALLAQEFQIDETWLEHNPMAKDVVMRLTHRSHPTIPLDLLF
                     AHDLQSQSALTRRRSLQLFGVSLYVCGPEDLILLKLKAGRPHDFEDALGIVKNPHTQL
                     DLDYLWNWADRLGLQGELQYVLHAADAGS"
BASE COUNT         1342 a         1783 c         1902 g         1307 t
ORIGIN      
        1 cgaggtccac aacgcgaaga ataatgagcg tcacgtttgt ggacgccgcc gagtcggtga
       61 ggcggcggtg tcttcgtgag ccggcagtgt tccgtgagcg ccccgtgttt tagtgagacg
      121 tccgtgtttg cgagaacaag gctggcggac tctttcggca tcctgctaga gtgagacctt
      181 gtgagggtct tgtgagcaag gaacgtatcg gggtgggact gatcggcgtg ggtcggcacg
      241 gggctcggta tgcccagcat cttgttcagg accttccaat ggcctctctc agagccgtat
      301 gtcgacgaca tcctgaacag ggttttcata tgccgggtgc cgagtcggtc acggtctatg
      361 gagatgccca atccctcatc gccgatccga cggtcgatgt ggtcgtcgtg gtgactccgc
      421 ccatttactc tttcgaaatt tgtggactag ccgcccacgc tcgtaaacct ctgttgatcg
      481 agaagccatt ggcgactacg gcaactgatg cccgtatgat ggtggctcaa gctcgcgaag
      541 ccggggtgcc cttgatgacg gcgcagaccc tccgattcga tcatacgatt cagcatatga
      601 agatgctcca accctctatc gggcattccc aacaactgga tttcgtgttt cacattgaga
      661 tacgaaaaac agttcccgac catgccgacg gctatggcaa acggggagcc gtgctggaaa
      721 tcggcattca tatgctggat ttggttcgat tcctaaccgg ggaagaaata cagaatgtgc
      781 agtgcacgat ggaccggcat ccaccgagtg cgccggaaat gagggcctct gttcacctca
      841 ccacgaccgg cgggacggcc tgccggatag acatcgcgcg agtcttgaga tcacgtgccg
      901 ggagagcgat atggatcggt tcacaggggc gggtggaagc cgactggatg catccccgaa
      961 ttcggtgtgt ggatgatgcc ggtgttcaga caatgaatgc cgatcctcct ccgtcccaga
     1021 ccgtcctcca gaccctcacg gcctttttgc aggcggtgaa aaacaattcc ccaatgccca
     1081 tcacggggga ggacgggtgt cgcgccgtgg aaatcgccga agcctgttac cgttcagcgc
     1141 aagcaggagg caagccgatt acgcttccgc ttcaacctca accttagcct cgatctgcag
     1201 cgctcggcta ggactctgcc acaatatggg tatccgtctc ttcgacaccg tcgattgcat
     1261 ggatcttcgt gatgacgacg tcggagagcg cccgttcatc ctggacgctg atgaagacaa
     1321 agatatccgg acggccgaag caggaatgag cctgttccac gccgggtatg cgtttcagtg
     1381 tgccgaccac gtccttggtc tttcccgcct tcactttgat gagaatgtaa gctctcgttg
     1441 ccacgtgtcc cctcctttga cgcctcagtc ggcggtggaa atattgttgt gagactgtct
     1501 cgcaggtctc cttgccctag tgacccgacg ctcccggcat cgcctttcga tggtgagcaa
     1561 acaccccttc aaaatcttgc cctcgcgcgg cgagcgccga ccgaccgtct cgcacgagct
     1621 gcatgaatcg cttgaaatca tcgaccgaga ggttgaattg gcggaaaccg gttgccagcc
     1681 gctccaactc ctccgcggag gtgcgctggt ccgcatccgc gagtgcttcc aatagttctg
     1741 cggtccatcc gcttgcgcga aatcgttcga gaacgggggt ggcctcttcg ccgtatttgt
     1801 cggttagggc gcgctgcgca aaatcctgta cggggcggtc cgtgcccttc gcgaaggcgg
     1861 attgaaactg aatgaccgac tgaatgaggc gatcggcttc ccgactgcct tccggtggaa
     1921 ggacctgtgc ttgttcgagc gtggcgagaa ctgccatagc cgacccgaca tgcggagagg
     1981 agcgctttgg ctgctgcacg gagagggaag gagtttggtc cgctgtgagc aaggaagacc
     2041 cgcctgccaa gggggaacag agtaagagag aaaggctaat ggcgaaaacc cggcaagcct
     2101 gccggttgga tcgtgaccca ctcaaaggga tcgttccctc tggggaatac agaggcgcat
     2161 gggcggatta tacagagtcg atgggagcgg tgctagagtg gacccggcgg cctgtcgctc
     2221 tattgaaacc cccttaaaga cttcctataa tcgctcggtt tggccgcggt ccagcccaca
     2281 tcaataatgt gggctagaaa ggcagtgttg tcatgcttgc caaggtgagg agcgcggcac
     2341 tcgtcggtct cgacgcgcat cttgtggatg tcgaggtcga tatctccggt ggtctccctc
     2401 aattctcggt cgtcggcttg cccgacgcga tcgtcaagga aagtcgtgac cgagtgcgct
     2461 ctgcgctgaa gaatacggga tttcactttc ctgccaaacg cattaccgtc aacctcgctc
     2521 ccgccgggat taagaaagag ggatcgggac ttgatcttgc gatcgccgtc gggatcctcg
     2581 tcgccgaaga agtcatccca cagtcgaggc tggaccatcg tgtgctggtc ggagaattgt
     2641 cgttggacgg tcgggtgaag ccgatcacgg gcgccttgtc ctttgcgctc gcttgccggg
     2701 tcggatacga cctgttgttg ccggccgaca atggtgctga agcagcatgg gtagacggcg
     2761 tcagtgccta tccgctccat agcctcccgg aagccgtgga gtttctgaat ggaacccaag
     2821 ccattgtgcc gagccggtcg aaccacgatg tgctgggggc ggccaggacg gcggaggatg
     2881 aagactattc ggatgtccgt gggcaagacc atgccaagcg ggcgctggag gttgccgcgt
     2941 ccggtggaca caatgtgctg atggtcggcc cgcccggttc cggcaagacg atgttggcgc
     3001 gccgattgcc ctcgattctt ccgctgctgg aatcggaaga agccattgaa acgacgcgta
     3061 ttcacagcgt cgccgggcaa cttgctccgg atcggccctt gctcgcggtg cggccctttc
     3121 gcgctcctca tcacagtatc tccgatgccg gtcttgtcgg aggaggcacg atcccgaaac
     3181 ctggggaagt ctcgcttgcg cataacgggg tgctgtttct ggacgagtct cctgagttca
     3241 aacggggggt gctggaggga ttgcgacaac cgttggaaga tggacagatg atcctgacca
     3301 gagcaagcgg aaccttgaaa tatccggcgc gattcatgtt gattgccgcg atgaatcctt
     3361 gtccttgcgg ctattatgga gaccgagcac gaccctgtat ttgtaccggc atgcagattc
     3421 gtcggtatcg cgcgaaactc tccgggccgc tgctggatcg gctggatatc cacctggatg
     3481 tgccgcctgt tccggtccga gagctccgta ctgaactgcc cgctgctgaa acatctgctg
     3541 cgatccgatc gcgtgtcgtc gcagctcggg agcgccaacg acggcgatat cgacatgacg
     3601 ggatctatac gaatgcgcaa ttgaagccgc gaatggtaaa gcggtattgt gggctcgacc
     3661 agccggctca ggagttgctc gagcacgcga tggcgaggct gcgcctgtcg gcacgggcac
     3721 atggacgcat tctacgggtc gcgcgcacca tcgccgattt ggccgactct gataagattg
     3781 agaccgtgca catcgcggaa gccattcaat accgttcctt tgaccgcaac cccgacatgt
     3841 gaggattcag tggcatcagt aaaagtgttt ttctggtggt ttgtcgtggg agcgacgatg
     3901 gcgctggccg tcatcctggt gcaagggggc atcagagaag tgatgcaggc gcaagggtct
     3961 gtgtgggaac tgaaactggt cgagctgctg accacgatca tgggcggcgg gttgctgggc
     4021 ggctgcatcg ccttgatcct cgatcggatt aaaaaatcct aagagtaaac tcctcacctc
     4081 cgtacagatt atccacgatc cgtttcctgc ctctgcccgc aaatcaaaaa ataccttcat
     4141 tcgtattgcc ttcttgtgat acgaaggagg tggccatccc ctccgagttc tgcgtcgttc
     4201 cgggttggaa gagtaaccga tgccgcgcaa tgaccaggct gttcggcagc tcatcatcct
     4261 caagaagctg gaatcgtccc ggcatggact gacgttggaa caacttgccg acagcttcgg
     4321 tccttcgaca actcgtcacc cacgaacgtt acgccgagat ctggacgcca tcgaatcggc
     4381 cggctatcca ctcctgactg aacgggtcga cggtcgtact cgctggaagt ttttagaggg
     4441 agcgcgccag gcgccggccc tccggttttc acccaccgaa ctcatggctc tcgctctcag
     4501 ccgccgtctc atcgcgccgc tcgaaggcac cgcgctgcat gcctcgctgg tatcagcctt
     4561 gagcaaagtc tcggctgccc tgccgccaga ggggctcgcg ctggtccaac aactggaagg
     4621 cacgttttca gtcgggcttg gcccgcacaa acgctacaag caccatcgcg agattgtcga
     4681 acgggtcacg caggccatcg ccgagaagcg gcgcgttcag atgcggtacg attccgcctc
     4741 tcgcaggcgc ataacccgcc gagaagtcga tccgtatcgc ctctggtacg cctccggcgg
     4801 tctgtacctc atcggctatt gtcacctgcg aaaagaaccg cgcatgttcg cggtggagag
     4861 gatcaaatcc gtcacgccca ccgagttgcc ttaccaaatt ccattgcatt tcgacttcga
     4921 tgccttcgtg gaagactcgc tcacggtcat gcgtggcccg cgcatcaatg tcgagttgga
     4981 gttcgacaaa gcgacggcag cctgggcgaa agatcgtgtc tggcatccca gccaagagtt
     5041 gaaacgactg cagggcggta agctgcggat cacattgtct gtagccgata gccgcgaact
     5101 cgtcggttgg atactcagct tcggcggcgg agtgaaggtg ctcagaccgg atgcgctgcg
     5161 tagggcggtc aaggaggaag ccagaaggat tctagagtcc caatgacctc ggatgtcacg
     5221 gcaggcgtag aaggagaagg atagcaggcc gtcatctcac gccaaactga gcggaagagc
     5281 gagccggtag aaaaatttca ggaccggacc tcggatgtca cagcgttatg tgaaactgga
     5341 cgtcatcgat gcgtcacaga aggaggcaat cgtgaaatgg cgtctgctga ttgcgatcat
     5401 gctgctcata ccgacgactg cgagccaagc cgagatgggc ttcgacccac gctacgagcg
     5461 ggactacaac atctttaatc cggtcacaaa gtaccagtcg gataatccac tcaatccggt
     5521 caacaaatac gatcccgaca acgccttcaa tccggtcaac cagtacgatc ccggcaatcc
     5581 gacaaacccg atcaaccaat acaacccgaa caaccccttc aacccggtga atcgttatca
     5641 tccggacaat cccttgaacc cggtgaacaa gttcaaccct agcgtgccgt tcgcgccgct
     5701 ggatggaagg agcagtcgga ggaccggagc atggtgatgc tggaaccgac acggatcgac
     5761 ggatcaactc ccggcgtcag cagcatggag gacatactgc agttcgcctt gaagcccaag
     5821 ccggtctgcc cagttccaga ggtagtccag gtcgagctgg gtatgaggat tcttcacgat
     5881 acccaacgca tcttcaaaat catgagggcg accggccttg agcttaagca ggatcaggtc
     5941 ctcaggacca cagacataca aagagacgcc gaaaagctga agggagcgtc tgcgagtaag
     6001 tgctgattga gattggagat cgtgcgcgaa gagaagatcc agcggaatcg tggggtgaga
     6061 acgatgagtc agacgcatca ccacatcctt ggccattgga ttgtgctcca gccaagtctc
     6121 gtcgatctga aactcctgag ccagcaacgc gtcgattact tcagtgcgaa tgggcaagtc
     6181 tgcgagcacg agcaagtcaa tatctttggt tgcgcgaagg cggccccacg cattgacggc
     6241 cagcgcacca acgagacaat acgaggtcga ttccggaagt gccgtgatga tggtgtgcag
     6301 gtagtcggcg agcgagtcgg ggctagcggg cgac
//
LOCUS       DATMTI010000647         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_449006, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000647 DATMTI010000000
VERSION     DATMTI010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449006"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VN039_10335"
     CDS             <1..459
                     /locus_tag="VN039_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWV46395.1"
                     /translation="SFLGESCLVGQTVRTATATTLEDSRILRIEKVAMLQMLQKQPRF
                     AEAFMSYLLVHSIRVQEDLVDQLFNSSEKRLARALLLLAHFGKESKPETVIAKISQET
                     LAEMIGTTRSRVSFFMNKFRKLGFIDYKGGLRRNGGLHVHSSLLNVVLHD"
     gene            904..>1839
                     /locus_tag="VN039_10340"
     CDS             904..>1839
                     /locus_tag="VN039_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV46396.1"
                     /translation="MSGETPRQRDLKNDKEGRQVCEPQQVATLATKGAEWSKTEHDLR
                     EEQKHLQRWNIELEQALNEKTAELRQSQGRLRALASELSLAEQRERKRLANELHDHLQ
                     QILVLGKLIIGQGKRFASGVPDCEIVLKKVDDILSEALTYSRTLVTELSPPVLHDYGL
                     AGGLRWLGDYMKKHGQTVTVMAPEDQNLKLPEGQVILLFQSVRELLINSSKHAGTSEA
                     TVRMEQRDDYLQIEVRDEGAGFDVAAADTPNGGISSKFGLFSIQERMRALGGSFTIDS
                     ALGQGTTAKLILRLARSAKDSELHFSGHQSRECSRN"
BASE COUNT          434 a          456 c          548 g          401 t
ORIGIN      
        1 agtttcttag gagaatcctg cctggtgggc cagacggtcc gtacggcgac cgcgaccacc
       61 ctggaagact cccgcattct ccgtatcgag aaagtcgcca tgcttcagat gcttcagaag
      121 cagccccgat tcgccgaagc atttatgtcc tatttattgg tacattcaat ccgagtccaa
      181 gaggatttgg tggatcaact gtttaattcc agcgagaagc ggttggcgcg ggcgctgctg
      241 ttgttggccc actttggaaa ggagagcaag ccggagacgg tcattgcgaa aatcagtcag
      301 gaaacgttgg ccgagatgat cggcaccacc cgctcgcgcg tcagtttctt catgaataaa
      361 ttccgcaagc tggggttcat tgattacaaa ggtgggctgc gccggaatgg cggattgcat
      421 gtgcatagct ccctccttaa tgtcgtgctt cacgactagg acctccatct gattccactt
      481 ctttgactct agcgagccag gcctggttgc gtcaggcctc gccaccattt tgtcatcatt
      541 cctactgagc aattgtgaac agacggccgc cgtctacttc attatgctga gccataacga
      601 agagagccaa ttcgatgggg ataggcccgg tgcactgtaa gctgccatat gtaaacgtaa
      661 tgcaatcatt actattcgta aattgtgggt gtatcgagga caccgtcgtt cgtgcgaatc
      721 gcttcagccg ttcggagaga atgcgaggtg tcccacgcag gcgcaggacc ctcgagggca
      781 aacattgatc cgatgagcgg atgctgcgac tgtgaatact tatctgacaa caaactatga
      841 tggaggggcc tccgcaggct aatgcctgcg caactctcct tctgatcgca ggaggcacac
      901 aatatgagcg gtgaaacccc gaggcagagg gaccttaaga acgacaagga gggccggcaa
      961 gtgtgcgagc cgcagcaggt tgcgaccttg gccaccaagg gcgcggaatg gagcaagacc
     1021 gagcatgact tacgtgaaga gcaaaagcac ttgcagcgtt ggaacatcga actggaacag
     1081 gcccttaacg agaagaccgc ggaattgcgc cagtctcagg ggcggctgcg ggcgttggcg
     1141 agtgaattaa gcctggctga gcagcgtgaa cggaaacggc tcgcgaacga actgcacgat
     1201 cacctgcaac agatattggt actaggcaag ctgatcattg ggcagggcaa gcggtttgcg
     1261 agtggtgtgc cggattgtga aatagtcttg aagaaggtgg acgacatctt gtcggaggca
     1321 ttgacatata gccgcaccct cgtgacggag ctgagccccc ctgtgttgca tgattatggt
     1381 ctggccgggg ggctccgatg gctgggggat tatatgaaga aacatgggca aaccgtgaca
     1441 gtgatggccc cggaggacca gaacctcaag ctgccggaag gccaagtcat tctactgttc
     1501 caatccgtgc gtgaacttct gatcaactct tcgaaacatg ccgggactag tgaagccacg
     1561 gttcgaatgg aacagcgcga cgattatctg cagatcgaag tgcgcgatga aggcgctggg
     1621 ttcgatgttg ctgctgctga tacacctaat ggcgggattt cttcaaaatt cggtttgttt
     1681 agtattcagg agcggatgcg ggccctgggc ggttcgttca ccattgactc cgctctgggg
     1741 caggggacca ccgcgaaact gattttgcgg ttggctagga gtgcgaaaga tagcgaattg
     1801 catttttccg gtcaccagag ccgggaatgc tcgcgcaac
//
LOCUS       DATMTI010000648         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_534498, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000648 DATMTI010000000
VERSION     DATMTI010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534498"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VN039_10345"
     CDS             <1..190
                     /locus_tag="VN039_10345"
                     /inference="COORDINATES: protein motif:HMM:NF037285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46397.1"
                     /translation="TGLPSSQAEYRLVTHSKTGRPDVTGWSPTTLRSTDGMVRQSLGS
                     TVQHQGLGGNASYWRKSA"
     gene            187..1086
                     /locus_tag="VN039_10350"
     CDS             187..1086
                     /locus_tag="VN039_10350"
                     /inference="COORDINATES: protein motif:HMM:NF041117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS oligonucleotide cyclase"
                     /protein_id="HWV46398.1"
                     /translation="MITLDDAFRKFRSRLELNTMEQGNASARHTEVREFLATKFDIDR
                     DFLTGSYVRHTKTKPLKDIDVLFELGDDERHYRKKAPSALIAAFRSALAEKYGNDKVK
                     SDGLAVNVSFGVQPDQDDNTDYRILSIDAVPAFWTGACYEIPDADTEHWIKTDPEIHA
                     ERATAANKAYSGEWKGLVRMLKYWNNNSKHGARPVKQSFMLEVMALDCLYGDWNGSFA
                     YEFQGLFATLADRIFDAWPDPAKLGPPVSDGMSAAEKTRARDLLQTASREASQAIHLA
                     RQGKNTEALAAWRTLFGPKFPLS"
     gene            1096..1872
                     /locus_tag="VN039_10355"
     CDS             1096..1872
                     /locus_tag="VN039_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46399.1"
                     /translation="MSRSDPVRNKFYVPLERADWASDMLFYIGASLSIAVLFVEKATH
                     PILYNVVMILFAVSVVALFGVGLATRVYFGPRATDQRTRDFLSSAYGVKLNYVVTDGY
                     YNNTLHEPTERLAAQVLENTHFSKAIAQEMAKAERIKFGVYVVLWLICLLARQVSLDF
                     ILAASQAVLSEQMISKWVRLEWLRSRCEHIYNDVYALFQLRSSGNMFDARVFEAFATY
                     ESVKANAATTLSGKIFEKKNSDLSSEWNEIKSALAIEQSP"
     gene            2020..2739
                     /locus_tag="VN039_10360"
     CDS             2020..2739
                     /locus_tag="VN039_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46400.1"
                     /translation="MPPIPGPIQSAVLAVLRAVREAGLGRINRTTLIKLVYLLDCLYA
                     ETHDGKTASGSSWYFHSFGPFASDLVGAIDEMASRGVIHDHSGEHGNKEYTQYWLGEY
                     PQGPSLADIGLGSTQSIRFGSLTKKFHNDLSGLLDHTYFKTLPMRDASPGRNIEFRGL
                     ADASQIKAHHQIGITDQAKLMKIILLSSRLENKFAEMEGNARAMAAHRPIYDRAYEEG
                     MAAMDADIHDESDVNFTAHLI"
     gene            2752..>3277
                     /locus_tag="VN039_10365"
     CDS             2752..>3277
                     /locus_tag="VN039_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46401.1"
                     /translation="MAFGNGTLVSEALPPYDQHPQSTTGLPRIGQIVRTVVPELGAAW
                     RIFDAARSDQTSHSSATGTIRGADSSRDWRPKPDRLPIFKIRVGDCAELLALTSKLRL
                     CVVLAQAEGVPDLKLPPPQRNLAREAFLRPSYLVAPLFCVGTPNEPRAVTPTIAARAE
                     CLVYPSLVFLPRTGG"
BASE COUNT          713 a          946 c          940 g          678 t
ORIGIN      
        1 gactggcctc ccttccagcc aggcggaata ccgcttggtt acgcacagta agacaggccg
       61 tccggacgtg accggttggt cgccaaccac gctgcgttcg acggacggca tggtgcggca
      121 gagtttgggc agtaccgttc aacaccaagg tctgggcgga aatgcgtcct actggaggaa
      181 atccgcatga tcaccctcga cgatgccttt cgaaagtttc gcagtcgcct ggagctcaac
      241 acgatggagc agggtaacgc ctcagctcga catacggagg tgcgcgaatt cctggcaacg
      301 aagttcgaca tcgatcgcga cttcctgaca ggttcctatg tccggcacac caagacaaaa
      361 ccgctgaagg acattgacgt gttgttcgag ttgggcgacg acgaacggca ctaccgcaag
      421 aaagctccgt ccgcgttgat tgcagccttt cgctccgcac tcgccgagaa gtacggcaac
      481 gacaaagtga aatcggacgg actagctgtc aacgtcagct tcggcgtgca gccggaccaa
      541 gacgacaaca cggactaccg catcctgagc atcgacgcgg tccctgcctt ctggaccggg
      601 gcgtgctacg agattcccga tgcagatacc gaacattgga tcaagacgga tcctgagatc
      661 cacgccgaga gagccaccgc cgcgaacaag gcctattcgg gggaatggaa agggctggtg
      721 cgcatgctta agtactggaa caacaacagc aagcacggcg cgcgtccggt gaagcagtcc
      781 ttcatgctgg aagtgatggc gctggattgc ctctacggcg actggaacgg tagcttcgcc
      841 tatgagttcc aagggttgtt tgcgaccttg gctgaccgga tcttcgatgc gtggccagat
      901 ccggcaaagc ttgggccccc ggtgagcgac ggcatgtccg ctgctgagaa gacccgagct
      961 cgtgaccttc tgcagaccgc aagtcgcgag gccagccaag ccattcatct ggcgcgacag
     1021 ggcaagaaca ctgaggcgtt ggcggcctgg cgaaccttgt tcggtccgaa gttcccactc
     1081 tcctgaggct cgcgcatgtc acgttctgat cctgtcagga ataagttcta cgttcccctt
     1141 gagagggctg attgggcgtc ggacatgttg ttctacatcg gtgcgtcgct gtcgatcgcg
     1201 gtgctgttcg tcgaaaaggc cacgcacccg atcctgtaca acgtcgtcat gatcttgttc
     1261 gctgtttctg tggttgcgtt gtttggtgtc ggcttggcga cgcgtgtcta tttcggtcct
     1321 cgcgcgaccg accagaggac tcgtgatttc ctgagcagcg cctatggagt gaagctgaac
     1381 tacgtggtta ccgatgggta ctacaacaat acgctgcacg agcctaccga acggcttgcg
     1441 gcgcaggttc ttgagaacac ccatttcagc aaggccattg ctcaggaaat ggcgaaagcc
     1501 gaacgcatca aattcggcgt ctacgtcgtg ttgtggttga tctgcctgct agcccgccag
     1561 gtgagcctcg acttcatcct cgcggcctct caggcggttc tgagcgagca gatgatttcc
     1621 aagtgggtgc gactggaatg gttgcggtct cgctgtgagc acatctacaa cgatgtctac
     1681 gcgcttttcc aactccgttc tagcggcaac atgttcgacg cccgcgtttt cgaagctttt
     1741 gctacttatg agagcgtgaa ggccaacgca gccacgacgc tttccggaaa aatcttcgag
     1801 aagaagaact cagatctcag ttccgaatgg aatgagatca agtccgcgct ggcgattgag
     1861 caaagtccct agtagcgccc taggtaggcc tcccctggcc atagttgtcg tcctccacaa
     1921 gctgatccac cgaggaatgc catcggcagc gtcccgcggt ttcgagttcg accacagtga
     1981 ggatagctat acaattccac gcgtcaccgg gcgccatcca tgccaccaat cccaggtcca
     2041 attcagtccg cagtccttgc agtgctacgc gccgtgcgcg aggcaggtct gggaaggatc
     2101 aatcggacga ctctcataaa gctcgtctac ctgctcgact gcctttatgc ggaaacgcat
     2161 gatggcaaga ctgcaagcgg ctccagttgg tacttccatt ccttcggtcc gttcgcatcc
     2221 gatctggtcg gagcgatcga cgagatggcg agccgggggg tgatacacga ccacagtgga
     2281 gaacatggaa acaaggaata cacgcagtat tggcttggcg agtatccgca aggcccgtct
     2341 ctggccgaca tcgggctcgg atcaacccaa tccatccgct ttggatctct gaccaagaag
     2401 ttccacaacg acctcagcgg ccttctcgac cacacttact tcaagacttt gccgatgcga
     2461 gacgcctcgc cgggtcgaaa catcgaattt agggggttgg cagatgcaag ccagatcaaa
     2521 gcccatcacc agatcgggat aacggaccag gcgaagttga tgaagatcat cctcctgagt
     2581 tcacgcttgg aaaacaagtt tgccgaaatg gaaggtaatg caagggcgat ggcagcacat
     2641 cgacccatct acgatcgggc ctatgaagag ggaatggctg cgatggacgc ggacatacac
     2701 gacgagtccg acgtgaactt cactgcgcac ttgatctaaa ggcaaacgag gatggcattc
     2761 ggcaacggga cattggtgag cgaggcactt ccgccttacg atcaacaccc tcagtcgacc
     2821 accggtctcc cgaggatcgg tcagattgtt cgcacggtag tccccgaact cggtgcagca
     2881 tggcgaatct tcgatgccgc ccgctcggac cagacctcgc atagctcagc gacgggtacg
     2941 atccgcggag ccgacagcag tcgtgactgg cgaccgaagc cggatcgctt gccgatcttc
     3001 aagatcaggg ttggcgattg tgccgagctt ctagcgctca cttccaaact tcgcctgtgc
     3061 gtcgtcttgg cgcaggccga aggagttcca gatctgaaac tcccgccacc acagcgaaac
     3121 ttggcgaggg aggcgttctt gcgtccctct tatttggtgg caccactgtt ttgcgttggc
     3181 acccctaatg agcccagggc ggtgacgccc accattgcag ctcgtgccga gtgtctggtc
     3241 tatcccagcc tcgttttctt acctcgtaca ggcggaa
//
LOCUS       DATMTI010000649         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_789060, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000649 DATMTI010000000
VERSION     DATMTI010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789060"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1192)
                     /locus_tag="VN039_10370"
     CDS             complement(158..1192)
                     /locus_tag="VN039_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035718769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HWV46402.1"
                     /translation="MTTEQKIIKTKVGVLELAKQLDNVSKACRLMGYSRDSFYRFKEL
                     YETGGEAALQEISRQKPLLKNRVSPEVEQAIVQLALAQPAWGQARVANELRKQALTVS
                     PAGVRCIWLRHDLETMRKRLKALEAKMAQEGLVLTETQLVALEKATADKEAHGEFESE
                     CPGYCGAQDTFYVGTLKGVGRIYQQTFIDTYSKVGFAKLYDRKTPVTAADLLNDRVVP
                     FFEQYEIALNRVLTDRGTEYCGAPDRHDYELYLAIENIDHTRTKTQRPQTNGICERFH
                     KTMLNEFYRVAFRKKIYRSLDELQTDLDGWLQDYNKQRPHQGRWCYGKTPMQTFIESV
                     PLAKEKMLAA"
     gene            complement(1265..2221)
                     /locus_tag="VN039_10375"
     CDS             complement(1265..2221)
                     /locus_tag="VN039_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWV46403.1"
                     /translation="MTYHKNARTTVYQRKRIRQSHAPYRVQAKALGVSVATVAKWTRR
                     PDVADRSSRPQRQYKALPPEAGPLLCWLRKDWLLDLDTVWLALRQAVFPQLSRSAVYR
                     ELMRLSLHQLHRLRPQPSHPRGRFRACPPGFLHIDTFALPRLGGPRRYLFVAIDRATR
                     LMTMQVATARNMASAVAFLKHCQRFYPFRLYRVLTDNGREFTLHGYHGRNGARTTKVH
                     PFTQSCRRSHIRHSLTKAYHPWTNGLVERTGGTIKAETVYRWHFNSTAMLDAALYGFE
                     RYFNEHRPYKAIGGKTPAQLTQDWYHRVPKRFLRPPFGALTT"
BASE COUNT          515 a          750 c          745 g          620 t
ORIGIN      
        1 cgtggctacc ttcattgatt ttgagcgacc acttcagacg aaacaggtgg aaagatttat
       61 gaaatacgca tccaacggtg tattgtgaag atcaccgaca tcctttcaat tgtataactc
      121 acaatttcat ctgacagaca atgagtcctc accgggttta ggccgctaac attttttcct
      181 tcgccaacgg cacactctca atgaacgtct gcatcggtgt cttcccgtag caccagcgcc
      241 cctggtgagg ccgctgtttg ttgtagtcct gcaaccatcc atccaggtcc gtctgtaatt
      301 cgtccaggga ccgatagatc ttcttccgga atgccacgcg ataaaactcg ttcaacattg
      361 tcttgtggaa ccgttcacag atcccgttgg tctggggacg ctgcgtcttg gtgcgcgtat
      421 gatcgatatt ctctatggcc agatagagtt catagtcatg gcggtcggga gccccacagt
      481 actccgtgcc acgatcggtc aggacgcggt tcaatgcaat ctcgtattgc tcgaagaacg
      541 ggaccacgcg gtcatttaag aggtccgccg ccgtcacggg cgtcttgcga tcgtacagtt
      601 ttgcaaagcc caccttgctg taggtatcaa tgaaggtctg ttggtagatc cggcccaccc
      661 ccttcaacgt tcccacgtag aatgtatcct gagcgccgca gtagcctgga cattcactct
      721 cgaattctcc atgggcttct ttgtcggctg tcgccttctc caaggccacg agttgcgtct
      781 ccgtaaggac gagcccctct tgagccatct tggcttccaa ggccttcagg cgtttccgca
      841 tggtctctag atcatgtcgc aaccagatac agcggacgcc ggctggcgag acggtcaggg
      901 cctgtttccg caattcattc gccactcgcg cttgtcccca cgccggttgg gcgagtgcca
      961 gctggacaat ggcctgttcc acctcagggg acacgcgatt tttgaggagc ggtttctgcc
     1021 gagagatttc ctgcagagcc gcctcgcccc cagtctcata cagctccttg aagcggtaga
     1081 agctatcccg gctatagccc atcaaccgac acgctttgga aacattgtcg agttgcttgg
     1141 cgagttccag cacccccacc ttggtcttga taatcttctg ctccgtcgtc atcgtcctcg
     1201 ctcctttcgt gaagaaggag gactctccct gactgtcaga ttaagttcta gctgtctagt
     1261 gtcactacgt tgtgagcgct ccgaacggtg gtcggagaaa tcgtttgggt acacggtgat
     1321 accagtcctg cgtcaattgc gcgggggtct tgcccccgat ggctttgtag ggtcgatgct
     1381 cgttgaagta ccgttcgaac ccatagagcg ccgcgtcgag catggccgtg gagttgaaat
     1441 gccagcgata gacggtctcg gctttgatgg tgccgccagt gcgttcgacc aagccattgg
     1501 tccaggggtg gtaggccttg gtcagactgt gccggatgtg gctgcgtcgg cagctctggg
     1561 taaatggatg gaccttggtc gtgcgagcgc cgttgcgccc atgatagccg tgcagcgtga
     1621 actcccgccc gttgtcggtc aacacccgat acaggcggaa cggatagaag cgttggcagt
     1681 gcttcagaaa cgcgacggca ctggccatgt tccgggccgt ggcgacctgc atggtcatca
     1741 ggcgagtcgc ccgatcgatg gcgacgaaca gatatcgtcg ggggccaccc agacgtggca
     1801 aggcaaaggt gtcgatatgc aggaatccgg gaggacacgc gcggaaccgc ccccgagggt
     1861 gactcggctg gggccgcagg cggtggagtt gatggagcga taagcgcatc agctcgcgat
     1921 agaccgccga ccgactgagc tgaggaaaca cggcctgccg caaggcgagc cacacggtgt
     1981 ccagatccaa tagccagtcc ttccgcagcc aacagagcag cggccctgct tcgggcggca
     2041 aggctttgta ctgacgttgc gggcgactgg accggtccgc cacatcggga cggcgcgtcc
     2101 atttggccac ggtcgccaca ctgacgccca aggctttggc ctgcacgcga tagggcgcat
     2161 ggctctggcg aattcgtttg cgttgataca cagtcgtgcg ggcattcttg tgataggtca
     2221 tggagccctc cttgatggtg agtgctccgg cccagcctat cggagtcaga cagattcgtc
     2281 atcatgtctg ttcacaatgt catgaaacta gacagctagc ctccagtcct ccgctcggag
     2341 cttcccttcg gcaagttttg tccgtagctt atctttatct tcctcggaaa gaagctgctc
     2401 cttcaccaag cggtccagta tcttatccgc gagttgtagc gagggcgatt ccatcattgt
     2461 gccaatatcc cgatagttgg tggagcggca tctttctgag cgaggcgctt ctcatagtct
     2521 cgccgattcg atttgatcaa cggcgcggcg agcgattcag taagcttgat ctccgtcttg
     2581 caattgggaa acatgatggt tggttcactc atgacggtgg ctcccttcac
//
LOCUS       DATMTI010000650         5702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_492122, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000650 DATMTI010000000
VERSION     DATMTI010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492122"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..2674
                     /locus_tag="VN039_10380"
     CDS             164..2674
                     /locus_tag="VN039_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV46404.1"
                     /translation="MQRGPTRSHPSVETARNTVAKACMKMPGRFPGLPTNAIIPPHVI
                     DRSDDRRIAIHSSTVISGVIAALFAAVFLLDVFTPLGVSVPVLYIVPLALTPLLQRRE
                     APYWLAGAASAATLCDYFFFHSHIDFPNWLPLSNRMISVSLVWLMAVIIRRQQDMADH
                     LAHTAALEAENHAHLVKEEALQRQADEIQDLYNRAPCGYHSLDATGRYVEVNDTELQW
                     LGYRREDIVGQHRFRDLLTPPSARRFDEVFPRFVAQGSIHDLELDLVGKDGSLLPVSL
                     SATAIRNSEGHYIASRSTIVDITERRRTEKVLRQAHAALETQVRDRTAELAIANRRLE
                     EQLQESRRTEEALRVSESRFRLTADHAPVLIWISDNTKACTWFNKPWLEFVGRRLEQE
                     VGNGWVDNVHPEDVARCVGIYVDSFDTRREFTMEYRLRRHDGAWRWILDHGIPRYEGD
                     GSFAGYIGSCTDIHDRKRAEELQGRLAAIVESSNDAIVSTSLDGTVRTWNEGAQRMFG
                     YGADEMVGRSIRTVIPADRHAEETELLGRVARGERIEEYETIRIRKDRTALDVSLTAS
                     PVRDPQGNLIGIAKIVRDITEHKKAETVLINKNKDLETLLYVTSHDLKEPLRAIESFS
                     QIVLERYADRIDERGGDYLRRIVRATQRLDRLLNDILELSRAQRMLPPMEEVEAEILV
                     KEALTRLDARIQETGAAVQIVYPLPRLRVNRMWATQGLYNLLANAMKFTRDGHPPDIE
                     IASYEASASTVPMTGVVVKDRGPGVPTRHAERIFMLFQRAVGRNVEGTGAGLTIVRQV
                     AERHGGRAWVQPRAGGGSEFVITFANSTHNGNGGASAT"
     gene            2671..3105
                     /locus_tag="VN039_10385"
     CDS             2671..3105
                     /locus_tag="VN039_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46405.1"
                     /translation="MNSIDILVVEDNDDDVVLIQAAFSEGKVINRLIIVRDGEEAMTY
                     LRKQDAYAEAPMPGIVLLDINMPKKNGFEVLADIKADPQLRALPVVMLTVSDREEDIV
                     RSFEQGACSYIRKPVSLTRFIAVVKEFELYWSLVSKVPNLNR"
     gene            3109..5547
                     /locus_tag="VN039_10390"
     CDS             3109..5547
                     /locus_tag="VN039_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46406.1"
                     /translation="MHVLLIEDNEDDAVLIRDVLSRQGGEPVTVDWADRLDTGLAQLA
                     HGPFDAILADLSLPDNHGLDIVSTLRRHRRDAPVIVLTGLDDAAVAERALRHGAQDYW
                     VKSRLSPDGLTRAIRYAIERHQAELALRRSEERFHLACRATRDAIWDWEIDADTLTWN
                     DTYRSVFGHSLPEKDNRMATWPERIHPEERAGVLAALHAAIQSSATIWTAEFRFLRTD
                     GTYAHALNRGYIVRSDDGVPRRMIGAMIDMSERRQLDRQRAAQLAVTIALDESVSLGE
                     AMPALLRVIGELKDWVFGSLWLVDTHKKVLRVDATWHSSSINADELTAAYRTACLRPG
                     IGLAGQAWKTALPLILADISEDTTFPTASMAQQAGLRGACAFPIYRGQDIMGVLEFHT
                     QEVLRADDEQRQMLADLGARISQLLHRKDLERQLRQSQKMEALGRMAGGIAHDFNNLL
                     TVINSWSALLLEHASMDEKWKRGLTHIKEAGDKASGLTRQLLAFTRQQVVEQQVMSLN
                     ERVMSIVELMQRVIGEDIHLIVNLDPHLWQVKADAGQIEQVIMNLVVNARDAMPQGGR
                     LELMSQNVRIEETDSARQEELHPGSYAVLTVRDSGCGMDEETLAHIFEPFFTTKERGK
                     GTGLGLSTVYGIIKQSGGYITVESELNSGTTFTIYLPQLVAGPPDPRSEPVVAPLLRG
                     SETVLLVEDDEMVLVLAQVVLEAQHYRVLPARNAEQAIELVRHHAQEIDVVLTDTVMP
                     GMGGPALAERLLQLRPGLRVIVTSGYAGRGQEFIQSLGSQAAFLQKPYTPDMLTKKLR
                     EVLDSSGAPRPIPD"
BASE COUNT         1182 a         1675 c         1726 g         1119 t
ORIGIN      
        1 tctcccctgc ctcggcatgg agtcggatca ccccccggaa ccccaggtac tcttactagg
       61 actgaaagag cctacgcggt acagccattg accagggctg aacgcatctg aattttcctt
      121 tgtttttcaa gcatactctc agacctatcg aggtgctttc ggtttgcagc ggggaccgac
      181 gagaagccat ccgtcggtcg aaaccgcgcg taacactgtc gcaaaggcct gcatgaagat
      241 gccggggcgc tttcctgggc ttccgaccaa tgcgatcatt ccccctcatg tgatcgatag
      301 gagtgacgac aggcgcattg ccatccacag ctcgacggtg ataagcggtg tgattgccgc
      361 cctcttcgcc gccgtctttc tgttggatgt ttttacgccg ctgggagtgt ctgtccccgt
      421 cctttacatc gtgcctctcg cgctgacgcc cttgttgcag cgccgcgagg ctccgtactg
      481 gctcgccggg gcagcttctg ccgcaacgtt gtgcgactac ttcttcttcc attcgcacat
      541 cgacttccca aactggctgc cgctcagcaa tcgtatgatt agtgtgtctc tggtgtggct
      601 catggcggtg attattagac gacagcagga catggccgac catctcgccc atacggccgc
      661 gctcgaggcg gagaaccacg cccatctcgt gaaggaggag gctcttcaga gacaagcgga
      721 cgaaattcaa gatctgtaca accgggcgcc ttgcggatat cactctctgg atgcaacggg
      781 ccggtatgtc gaagtcaacg atacggagct gcaatggctt ggataccggc gcgaggatat
      841 cgtcggtcaa caccgctttc gagatctttt aactcccccg agcgcccgcc ggttcgatga
      901 agtattccca cgtttcgtcg cccagggatc catccatgac ttggagctgg acctcgtggg
      961 aaaagatggt tcgctcctgc ccgtttcgct gagcgcaacg gcgatccgga attcggaggg
     1021 ccattacatc gccagccgat ccacgatcgt cgatatcacc gagcgccggc gcacggaaaa
     1081 ggtgttgcgg caggctcacg ccgcgctgga gacccaggtc cgggatcgaa cagccgagct
     1141 ggccattgcc aaccgccgtt tggaggaaca actccaagaa agccggcgga cggaagaggc
     1201 gctacgggtg agcgagagtc gcttccgtct cacggccgat cacgcacccg tcttgatttg
     1261 gatcagcgat aacaccaagg cctgcacctg gttcaacaag ccgtggctcg aattcgtcgg
     1321 ccggcgcctg gaacaagaag tcggaaatgg atgggtggac aacgttcatc cggaggacgt
     1381 ggctcgatgc gtcggcatct atgtcgactc tttcgatacc cgacgcgaat tcacgatgga
     1441 ataccggctc cggcggcacg acggagcatg gcgctggatc ctcgaccatg gcatcccccg
     1501 ctacgaagga gacggttcct tcgccggcta tatcggttcc tgcaccgata ttcatgaccg
     1561 caagcgcgcc gaggagttgc agggccggct ggcggcgatt gtggagtcgt cgaacgacgc
     1621 catcgtcagc acctccctgg acggaaccgt ccgcacctgg aatgagggcg cacagcgaat
     1681 gttcggctac ggcgcggatg aaatggtcgg ccgatccatt cgcaccgtca ttcccgccga
     1741 ccgccatgcg gaggaaacgg aactgctcgg acgagtggca aggggcgaac gcatcgagga
     1801 gtatgaaaca attcggatca ggaaggaccg tacggcgctc gacgtctccc tgaccgcttc
     1861 tccggtccgc gacccgcagg gaaacctcat cggcatcgcc aagattgtgc gcgatattac
     1921 cgagcacaaa aaggccgaga cggtcctcat caacaagaat aaggatctcg aaacgctctt
     1981 gtacgtgacg tcccacgact tgaaagagcc cctcagggcc attgagagtt tttcccaaat
     2041 tgtgctggaa cgctacgcgg atcggatcga tgagcgcggc ggtgattacc tccgacgcat
     2101 cgtgcgggcg acacagcggc tggaccgcct cttgaacgat attctggagc tctcgcgtgc
     2161 ccagcggatg cttccgccga tggaagaagt ggaggccgaa attctggtga aggaggcgct
     2221 gacccggctg gacgcccgca ttcaagagac cggtgcggca gtccagattg tgtaccctct
     2281 gccgcggttg cgcgtgaatc ggatgtgggc cacacaaggc ctgtacaacc tgctggcgaa
     2341 tgcgatgaag ttcactcgcg acggccaccc gccggatatt gagatcgctt catacgaagc
     2401 ttccgcttct acagttccca tgaccggagt cgtcgtgaag gaccgaggtc ccggggtccc
     2461 gactcggcat gccgaacgga ttttcatgct gtttcaacgg gcggtgggac gaaatgtgga
     2521 gggaaccggc gccggactca ccatcgtacg ccaggtggcc gaacgtcacg gcggccgagc
     2581 gtgggtccag ccacgcgccg gcggcggatc agagttcgtg attactttcg ctaattcgac
     2641 tcacaacggc aatggagggg catcggcgac atgaattcga tcgacattct cgtggtggag
     2701 gacaatgacg acgacgtcgt cctcattcag gcagccttct cggaaggtaa ggtgatcaac
     2761 cggctcatca tcgtgcgcga cggggaggaa gccatgacct acctgcgcaa gcaagatgcc
     2821 tatgccgagg ctcccatgcc cggcatcgta ttgttggata tcaacatgcc gaagaaaaac
     2881 ggctttgaag tgttggccga catcaaggca gatccacaat tacgtgccct tccggtcgtc
     2941 atgttgaccg tcagcgatcg agaagaagac atcgtccgat cgttcgagca aggtgcttgc
     3001 tcgtacattc gcaaacctgt cagcttgacg cgattcatcg ccgtcgtgaa agagttcgaa
     3061 ctgtattgga gtctcgtctc aaaggtcccc aacctcaata ggtgaccgat gcatgtactg
     3121 ttgatcgagg acaatgaaga cgatgcggtc ttgatccggg acgtcctgtc acggcagggc
     3181 ggcgagccgg tcaccgtgga ttgggcggac cggctggata cgggattggc gcaactggcc
     3241 cacggcccct tcgacgccat cctggccgat ctgtcccttc ccgacaatca cggcctggac
     3301 atcgtgagta cgctgcgccg gcacaggcgc gacgctccgg tgatcgtcct gacggggctg
     3361 gacgacgcag ccgtcgccga acgggcactc cggcatggag cgcaggatta ttgggtcaag
     3421 tcgcgattga gccccgacgg cctcacgcgc gccatccggt atgccatcga gcggcatcag
     3481 gccgagctgg ccctccgccg gagcgaagag cgcttccact tggcctgtcg cgccaccagg
     3541 gatgccatct gggattggga gatcgacgcg gacacattga cctggaacga tacgtatcgg
     3601 tcggtcttcg gacatagtct ccccgaaaaa gacaatcgga tggcgacctg gccggaacgt
     3661 atccatccgg aagaacgagc cggcgttctc gccgctctcc atgcggccat tcaatcgtcg
     3721 gccaccatct ggaccgccga attccgcttt ttgcgtacgg acgggacgta cgcgcacgca
     3781 ctcaaccgcg gatatatcgt gcggagcgac gacggtgttc cccggcgcat gatcggcgcg
     3841 atgatcgaca tgagcgagcg ccggcaattg gatcgccaac gcgccgccca gcttgccgtg
     3901 acgatagcgc tcgacgaatc ggtctcgctc ggtgaggcga tgccggccct tctgcgcgtc
     3961 atcggcgaac tgaaagattg ggtgttcgga tcgctctggc tggtcgacac ccacaagaag
     4021 gtcttacgcg tcgatgcgac ctggcatagc tcgtcgatca atgcagacga attgacggcc
     4081 gcctaccgca cggcctgcct acgccccggc atcggtctcg cgggacaggc atggaagacg
     4141 gctcttccgc tgattctggc ggacatttcg gaagatacaa cttttcctac cgcctctatg
     4201 gcgcaacaag ccgggttgcg cggagcctgc gcttttccga tttaccgggg acaggacatc
     4261 atgggcgtgt tggagttcca cacccaagag gttctgcggg cggacgacga gcagcgccaa
     4321 atgctggccg acctgggggc gaggatcagc cagcttctcc atcgcaaaga tctcgagcgc
     4381 cagcttcgcc aatctcaaaa aatggaggcc ttgggacgga tggccggtgg gatcgcacac
     4441 gacttcaata atctgttgac ggtcatcaat agctggagcg cgctcctgct cgaacatgct
     4501 tccatggatg agaagtggaa gcggggactc acgcacatca aagaggcggg agacaaggcg
     4561 tccggcttga cgagacaatt attggcgttc acacgccagc aagtcgtgga acaacaggtc
     4621 atgagcctga atgagcgggt catgagcatt gtcgaattga tgcagcgggt gattggcgag
     4681 gatattcatc tcatcgtgaa cctggaccca catctctggc aggtgaaggc cgatgccggc
     4741 cagatcgaac aggtcatcat gaacctggtg gtcaatgccc gcgatgccat gccgcaaggc
     4801 ggtcgattgg agttgatgag ccaaaatgta cgcatcgaag agacggactc cgccagacag
     4861 gaggaactgc acccgggctc ctatgccgtt ctgacggtgc gggatagcgg atgcgggatg
     4921 gatgaggaaa cgctggctca tatttttgag ccgttcttca caacgaaaga gcgaggcaaa
     4981 ggaaccgggt tggggttgtc gacagtgtac ggcatcatta agcaaagcgg cgggtatatt
     5041 acggtcgaga gcgagctgaa cagcggcacc acattcacca tctatcttcc ccagctcgtc
     5101 gccggaccgc cggacccgcg ttccgaaccc gttgttgccc cactcttgcg gggctcggag
     5161 accgtactgc ttgtggaaga cgacgagatg gtcctcgttc tcgcgcaggt ggtattggag
     5221 gctcagcact atcgggtctt gcccgcgcgc aatgccgagc aagccatcga acttgtccgg
     5281 catcatgccc aggagatcga cgtggtcctg accgatacgg tcatgccggg tatgggaggt
     5341 ccggctctgg ccgagcggct gttgcagttg cgtccgggcc tgcgcgtgat tgtgacctcg
     5401 ggctatgccg ggcgcggaca ggagttcatc cagtcgctgg gatcacaagc cgcgttcttg
     5461 cagaagccct atacgccgga catgctgacg aaaaagctcc gcgaagtctt ggattcgagc
     5521 ggcgcaccgc gtcccatacc ggactgacgg caccaggcct tggtgagaag ccgaacaccg
     5581 cctttcttca ttgcgtcctt ttctaacctc ctccgtctat agagctgagt gcggaatcat
     5641 ccgctactcg agaacaagga ggtttcccat gcgtccccat atttctcttg atgtccgtga
     5701 tg
//
LOCUS       DATMTI010000651         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_534579, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000651 DATMTI010000000
VERSION     DATMTI010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534579"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1467)
                     /locus_tag="VN039_10395"
     CDS             complement(178..1467)
                     /locus_tag="VN039_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV46407.1"
                     /translation="MRVGFVLVCAISLGLDVATASAQSRLGTDTASVPSDGRPYSLDM
                     IIALALAKNPVVGLAEGNIEQQKGQQTAAGAYPNPTVTGHGGHGKLKDTIVGPPENTT
                     STTEYNVVVGQPIEWPGMRAARQRTADLGLATANVGMLETQLNLASQVKVSFYDLLLA
                     QQGADLARQNLDIVEGVARIVKARVKSGEAPQFESIKAEVEVLKARQQLVRADNLVRI
                     NRVAVDTLTGGALGPFYMIYGEFRIIPRDLHIEGLMARMVEQHPTIQRLLKSVEQSDW
                     KIEFERQARVPTLTVNGSYWREIGREAYQGGLSVPVPLWYRRQGEIASSLGAKRREEA
                     ELLRIRNELGRAVHQHYQDVHTTAELIDVFDKGLLKQAQEALRLAQFSFQQGASSLLE
                     VFDAQRVQRQILVDYAQARHDLSVSLARLEQAVGGTL"
     gene            complement(1534..>2230)
                     /locus_tag="VN039_10400"
     CDS             complement(1534..>2230)
                     /locus_tag="VN039_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWV46408.1"
                     /translation="YPQSILSLIVSPRFLRAETEADRRARTKLPARQFICPDTGFEKL
                     TDVEAPQGILAVVRQPGWDEAQVFRRTSALGIYGDRLQDPANVGTIIRTAAALDLSGV
                     WLSADSADPFSPKVVRATAGAILSLPVFHTRDFRVFSSYGCEIYSAVLASADIVPIRT
                     VRTIPSRLMIAVGNEGAGLDPDIVKASSVKFSIPLAEGVESLNVAATAAISAFYFSGL
                     RPDSGVGSISCSA"
BASE COUNT          498 a          689 c          574 g          469 t
ORIGIN      
        1 cttccgccga caggataatg aaacccggag ttgcgactgc gacaggcgct ttactggcca
       61 ctacatcgtt cggcgttcca tcacaggctg attccatcac catcaaaccg aacagaagac
      121 cgcgcatgct ccggctgatg gataacgacg gacttcgcat tgaaccgaat agaccgttca
      181 caatgttccc cccaccgcct gttcaagccg agccagcgag acagagaggt cgtgccgtgc
      241 ttgagcatag tccactaaga tctgccgttg tacacgctgc gcatcgaaca cttcgagcaa
      301 gctggacgct ccctgctgga aactaaactg cgcaagccgc agagcctctt gggcctgctt
      361 cagcaaccct ttgtcgaaca catcgatcaa ctccgccgtg gtgtgaacat cctgataatg
      421 ttgatggaca gctcgcccca gttcgttacg gatccggagc agctcggctt cttcacgacg
      481 ctttgcgcct aacgacgaag caatttctcc ctgacgtcgg taccagagcg gcacggggac
      541 agagagtcct ccttgatacg cttctcgccc gatctcacgc caataactgc cgttgacggt
      601 gagtgtcggc acccgcgcct gccgttcaaa ttcaatcttc caatccgatt gctcaacgga
      661 cttcagcaag cgttggatag tcggatgctg ctccaccatg cgggccatca aaccttcaat
      721 gtgaagatca cgaggaatga tcctgaattc accgtagatc atatagaatg gcccaagcgc
      781 accaccggtc aaagtgtcga cggcaacgcg gttgatgcga accagattat ccgcccgtac
      841 cagttgctgc cgtgccttca gaacttccac ttccgccttg atggattcaa attgaggcgc
      901 ttcgcccgat ttgacccttg ccttcacgat ccgcgccaca ccttcgacga tgtcaagatt
      961 ctggcgcgcg aggtcggcgc cctgctgagc cagcagcaga tcatagaagg acaccttcac
     1021 ctgcgatgcc aaattcaatt gggtctccaa catgccgacg ttggccgtcg ccaatccgag
     1081 gtctgccgtt cgttgccgag cggcccgcat tcccggccat tcaatcggtt gcccgactac
     1141 cacgttgtat tcggtggttg acgtagtatt ctccgggggt cccacgatcg tgtctttgag
     1201 ctttccgtgc cctccatgac cagtcacagt gggattagga taggcaccgg ctgctgtctg
     1261 ctgccccttc tgctgttcaa tgtttccttc agccaaaccg acgacaggat ttttcgccaa
     1321 cgcgagagca atgatcatgt cgagactgta cggccgccca tcggatggga cagaggccgt
     1381 gtctgtgcca agcctggatt gggcagaggc cgtggccacg tcaagcccaa gactgatcgc
     1441 acataccagc acgaaaccta cccgcattct tgtctcctgt ttagacatca tgaaccgcat
     1501 atagtaggcc gaatgtacct atatttcaaa tctctacgcc gagcagctta tcgatccaac
     1561 tccggaatca ggtcgcaatc cgctgaagta gaacgccgaa atcgccgctg tcgcggcgac
     1621 attcaacgac tccaccccct cggccaatgg aatggagaac ttgacgctgg acgccttcac
     1681 gatgtccggg tccaaccccg ctccctcgtt tccgaccgca atcatgaggc ggctgggtat
     1741 tgtccgaaca gtcctgatgg gaactatatc agccgaagcc aagaccgccg aatagatttc
     1801 acatccatac gaggagaata cgcgaaagtc tcgagtatga aagaccggaa gactcaggat
     1861 ggcgccggct gtggcacgga caaccttcgg gctgaacgga tcagccgaat cggcgcttaa
     1921 ccacactccg gatagatcca aggctgcggc ggtccggatg atcgtgccga cattggccgg
     1981 atcttgtaac cgatccccat agatgcccaa cgcgctcgtt cgcctgaaca cctgcgcctc
     2041 gtcccaccca ggttgtcgca cgacggccag aatcccttgc ggcgcctcca catcggtgag
     2101 tttttcaaag ccggtgtccg gacaaataaa ctggcgcgca ggcaattttg tgcgcgccct
     2161 tcggtctgcc tcggtctcgg cacggagaaa acgcggcgat acgatcaggc tgagaataga
     2221 ctgtgggtac
//
LOCUS       DATMTI010000652         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_238300, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000652 DATMTI010000000
VERSION     DATMTI010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238300"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /gene="lysA"
                     /locus_tag="VN039_10405"
     CDS             <1..1348
                     /gene="lysA"
                     /locus_tag="VN039_10405"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWV46409.1"
                     /translation="PIAAGGNTVNGIGSDVIDVTQGTHAMHSFEYQHGELYCEQVPVS
                     RVAKELGTPCYIYSHATLVRHFHAYDSAFKNIPHVIAFAMKANSNLAILRMMAKEGSG
                     ADIVSGGELFRALKAGVPASKIVFAGVGKSPDEIRDALKADILMFNVESSAEIHAINE
                     VASSVGKKARIALRINPDVDPKTHPYISTGMKKSKFGIASDRALEEYKMASSLSHTDV
                     VGVHAHIGSQLTDVMPFVDSLKKVVALIDTLKSQGINIRYLNIGGGLGITYSEEKPPL
                     PQDLAEAISPLVKDLGLTLVMEPGRVIVGNAGILVTRTLYEKAGETKHFVIVDAAMND
                     LIRPSLYGAYHEIRPVQEEAIRRAKQTVDIVGPVCESGDFLAKDRSLPGVKPGELLAV
                     MSAGAYGFVMASNYNSRPRVPEILVKGGEYHVIRDRETYDDLIRGESIPSFLSETE"
     gene            1357..2229
                     /gene="dapA"
                     /locus_tag="VN039_10410"
     CDS             1357..2229
                     /gene="dapA"
                     /locus_tag="VN039_10410"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWV46410.1"
                     /translation="MFAGSLVAIVTPFRQGKVDERALAELIEWQIANGTNGIVPCGTT
                     GESATLSHSEHNRVIELTVEVVRRRVPVIAGTGSNSTEEAIALTKHAKQAGADGALLI
                     TPYYNKPTQEGLYQHYRAVAENVDLPLVLYNIPSRTGVNMLPSTIARLSVINTIVGVK
                     EGSGSVQQASDIVQQCGDRLTVLAGDDSLTLPMMAVGGKGVITVTANIIPLEMAGLVK
                     AFADGRIDEARRIHFKLSPLFAALFFETNPIPIKEALGLMRKIDPELRLPLCPMAQDT
                     REKLIRVLKDAALI"
     gene            2244..3062
                     /gene="dapB"
                     /locus_tag="VN039_10415"
     CDS             2244..3062
                     /gene="dapB"
                     /locus_tag="VN039_10415"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWV46411.1"
                     /translation="MHDNKMIKVIVAGAAGRMGSRLISLIRDSTALTMAGALEGKGHP
                     SLGEDAGETAGSGRTGLLITDDLSILMERGEVVIDFSSPVATLEHMRTVAHHRRAMVI
                     GTTGFSAVQLDELKSLAQQIPCVFSPNMSVGINLICKVIGEMARTLGDDYDIEVIEAH
                     HRLKKDAPSGTALKIAEVLARSVNRDLSQVGVYARKGLIGERTKGEIGIQTIRAGDIV
                     GDHTILFGGMGERIELTHRASSRDTFVQGALRAARWVVRQPAGLYDMMDVLSLR"
BASE COUNT          733 a          838 c          907 g          677 t
ORIGIN      
        1 ccccatcgca gccggtggga acacggtaaa tggtattgga tctgatgtaa ttgatgtaac
       61 ccaaggaacc cacgcgatgc atagcttcga atatcagcac ggtgaattgt actgcgaaca
      121 ggtaccggtc agccgcgtgg cgaaggagct cggcactccc tgttatatct atagccatgc
      181 gacgctcgtc cggcatttcc acgcctacga cagtgcattc aaaaatatcc cgcatgtcat
      241 cgcgtttgcg atgaaagcca attccaatct ggccatcctt cgcatgatgg cgaaggaagg
      301 cagcggcgcg gatatcgtgt cgggcggcga gctgttccga gccctcaaag ccggcgtgcc
      361 ggcttccaaa atcgtttttg ccggcgtcgg taaatccccg gatgaaattc gcgacgcgct
      421 gaaggccgac atcctcatgt tcaatgtcga gtcgtcggct gagatccatg cgatcaacga
      481 ggtggcgtct tcagtcggga aaaaggcacg gatcgcgtta cggattaatc cggatgtaga
      541 ccccaaaaca catccttaca tttcgacggg gatgaagaaa agcaagttcg gcatcgcctc
      601 ggatcgcgcg cttgaagaat acaaaatggc gtcctcgctg agccataccg acgtagtagg
      661 cgtccatgcc cacatcggct cgcagttgac ggacgtgatg ccgttcgtcg attcactcaa
      721 gaaagtcgtg gctctcatcg atacgctgaa gagccagggt atcaacatcc gttacctgaa
      781 catcggaggc gggctcggca tcacctattc cgaggagaag ccgccgttgc cccaggacct
      841 ggctgaggcg atttcgccgc tcgttaagga tttggggctg acgctggtca tggagccggg
      901 ccgcgtcatc gtcggcaacg ccggcatcct ggtaaccagg acgctgtacg agaaggccgg
      961 ggagacgaag cactttgtca tcgtcgatgc cgccatgaac gatttgattc gaccgagctt
     1021 gtacggtgcc taccacgaaa ttcgccccgt gcaagaggaa gccatccgtc gggccaaaca
     1081 gacggtggat atcgtcggac ctgtgtgtga atcaggagat tttttggcca aggatcgatc
     1141 gttgccgggt gtcaaacccg gtgagttgtt ggcggtcatg agcgcgggag catatggctt
     1201 tgtgatggca tcgaattaca actctcggcc tcgtgtgccg gaaatcctcg ttaagggagg
     1261 agagtatcac gtcattcgag atcgagagac atacgacgac ctcatacgag gcgagagcat
     1321 tccatcgttc ctcagcgaaa cggagtgagg gtaagtatgt ttgccggatc gcttgtcgct
     1381 attgtcacac cgttccgaca gggcaaagtg gacgaacgtg ctttggccga gttgatcgag
     1441 tggcagattg ccaatggcac caacggcatt gtcccctgcg gaaccacagg tgagtcggcc
     1501 acgctttccc atagcgagca taatcgtgtg attgagttga cggtcgaggt ggtccgccga
     1561 cgggtaccgg ttattgcagg aaccggttcg aacagcacgg aagaagccat cgcgctcacg
     1621 aaacatgcga agcaggctgg ggccgacggc gcgttgctca ttacccctta ctacaacaaa
     1681 ccgacccagg aaggactcta tcaacactat agagcggttg cggagaacgt tgacctgccc
     1741 ttagtcctat acaacatccc gagtcgcacc ggagtcaaca tgttaccgtc aacgatcgcc
     1801 cgactctcag tcattaacac gatcgtcggg gtcaaggaag gaagtggctc cgtccaacag
     1861 gcttcagata tcgtgcagca gtgcggtgac cgcctaaccg tgctggctgg cgacgattcc
     1921 ctcacgttac cgatgatggc ggtcggtggg aagggtgtga ttactgtaac ggccaatatc
     1981 ataccgttgg aaatggccgg ccttgtgaaa gcctttgccg atggcagaat cgacgaagcg
     2041 cgacggattc attttaagct ctctcccctc tttgcggcgt tgttctttga gaccaatccc
     2101 attccgatca aagaagcgtt gggactcatg aggaagattg acccggaatt gcgcttgccg
     2161 ctctgtccaa tggctcaaga tacgcgcgag aaattgatcc gcgtcctgaa agatgcggcg
     2221 ttgatctaac gagcaatatc tagatgcacg acaacaagat gatcaaggtt attgtagcgg
     2281 gtgcagcagg ccgaatgggc agtcgactga tatcactgat cagagattct acggctttga
     2341 ccatggcagg agccttagaa ggaaaagggc atccatcgtt gggagaggat gcgggcgaga
     2401 ctgcaggatc aggccgtacc ggccttctca tcacggatga tctctccatt ctgatggagc
     2461 gaggagaagt ggtgatcgat ttttcctcgc ccgtggcgac acttgagcat atgcggactg
     2521 ttgctcacca tcgacgggcc atggtaatcg ggactaccgg cttttctgct gttcaactcg
     2581 atgagttgaa atcactggcc cagcagattc cctgcgtctt ttctccgaac atgagtgtcg
     2641 gaatcaacct gatctgcaaa gtcatcggcg agatggccag aacacttggg gacgattatg
     2701 acatcgaggt cattgaagct caccacaggc tgaagaagga cgcgccaagc ggcacggccc
     2761 tcaagattgc cgaagtcctc gctcgctcgg ttaatcgaga cttgagccaa gtcggagtct
     2821 atgcccgcaa ggggttgatc ggtgagcgaa caaaaggaga gatcggcata caaaccattc
     2881 gcgccggcga tatcgtcggc gatcatacga ttctgttcgg cggtatggga gaacgaatcg
     2941 agctcaccca ccgagccagc agccgcgata cgttcgtcca gggagccctg cgagccgcga
     3001 gatgggttgt ccgccagccc gccggcttat acgacatgat ggatgtctta agtcttcgtt
     3061 gattcctcct tcgtcgcttc acatcaccat aagcagtcga ttgtgttttc agggaacccg
     3121 accaagtact tgcagtgcgt tcatccatcg gctcc
//
LOCUS       DATMTI010000653         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_66631, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000653 DATMTI010000000
VERSION     DATMTI010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66631"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VN039_10420"
     CDS             <1..802
                     /locus_tag="VN039_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWV46412.1"
                     /translation="DIGTSKELIEQHSLCAGCPESMAFRYILASLPNPEDTVMVGSTG
                     CTSLVFPMVAVHNIHSLFGNQNAIASGLKRALSVRFPGRVKDVVVLAGDGATVDIGLD
                     MTLQAWFRQEKFTTICFDNELYANTGGQESGLMQKGFVAKMAPVGKLFDKVRLPEIAR
                     ESGCHYVVNCTVSKPSLVEKVIRNAVHIAREIGPTYLQLYTPCILEIGKNSMEGLQEM
                     RDSEKPTERFAYKEYISEPAKQLLTELAAKDKERKAAAKQLAAQAQAN"
     gene            817..1524
                     /locus_tag="VN039_10425"
     CDS             817..1524
                     /locus_tag="VN039_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWV46413.1"
                     /translation="MIKKRLNIRMSGLGGQGAVTAAHVMAMAANRDGKFSISNPFFGA
                     EKRMAPAESYCRIGIERIYDRGELVFPDVIEVFHPQVITMGKSYTMPFYSGIKEGGVV
                     IINSGQSLLSEEDIERLKDLNVATFYIAGTELAIETAGTELSTNMTMIGSVAGITKCV
                     SMEALDGALQERFGKKFVASGGTASLDEAIKKKFAKKEMLLAKNLATVKRAYEIGAEW
                     AEKNKIELRVGNPAVAA"
     gene            1543..1821
                     /locus_tag="VN039_10430"
     CDS             1543..1821
                     /locus_tag="VN039_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HWV46414.1"
                     /translation="MYNVAQVIDEKCTAKKGCRLCIMYCPEANCLDLNTTKMVAEVII
                     DRCKGCELCVVVCNAAKHEAIMMQAVSATGQLMSKKGESAALGQAYQG"
     gene            1897..2166
                     /locus_tag="VN039_10435"
     CDS             1897..2166
                     /locus_tag="VN039_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46415.1"
                     /translation="MESEDNVIDELLREISGLIGEYPKAIERRAAAIQATGKDPELVE
                     KLVKAADTMRDSGNLYLTWARHYAALAEGNTDASSDEDETEDFDV"
BASE COUNT          586 a          558 c          589 g          469 t
ORIGIN      
        1 agacatcggg acatctaaag agctgattga gcagcattcg ctctgcgccg gctgccctga
       61 gtccatggcc ttccgctaca tattggcctc cctcccgaat ccggaagaca cggtcatggt
      121 cggatcaacc ggctgtacca gcttggtatt cccaatggtc gccgttcata atattcattc
      181 gttgttcgga aaccagaatg ccatcgcatc agggctcaag cgcgccctca gtgtcagatt
      241 tccgggtcgt gtaaaagacg tcgtcgtcct tgccggcgac ggtgcgactg tcgatatcgg
      301 attagacatg acgctccaag cctggttccg tcaagaaaaa ttcacgacaa tttgcttcga
      361 caatgagctc tatgccaaca ctggcgggca ggagagcggg ctcatgcaga aaggctttgt
      421 cgctaaaatg gcaccggtcg ggaagttgtt cgacaaggtg cgcttgcctg agatcgctcg
      481 cgaatccggc tgtcattacg tcgtaaattg caccgtcagc aaaccgtcgc tggttgaaaa
      541 ggttatccgc aacgccgtgc atattgcccg tgaaatcggt ccgacctatc tccagctcta
      601 tacaccctgt attctcgaaa tcggcaagaa cagcatggaa ggcctgcaag aaatgcgtga
      661 ctccgaaaag ccgaccgagc gtttcgcgta caaggaatac atcagcgaac cggcaaagca
      721 actgttgact gaactggccg ccaaagataa agagcggaag gcagctgcta aacagttggc
      781 tgcgcaggct caagctaatt agaccggagg cccttcatga tcaaaaagcg acttaatatc
      841 agaatgtcag gattaggcgg acaaggcgcc gtcaccgccg ctcatgtcat ggctatggcg
      901 gccaatcggg atggcaaatt ctccatctca aacccgttct tcggtgcaga gaagcgcatg
      961 gcgccggctg aaagctattg ccgcatcggc atcgagcgga tctatgatcg cggcgaactc
     1021 gtgtttccag atgtgattga agtattccat ccccaggtca tcacaatggg gaaaagttat
     1081 acaatgccgt tctactcagg gattaaggaa ggcggcgtcg ttattatcaa ttccggtcaa
     1141 tccctgttgt cggaagaaga catcgagcgc ctcaaggatc tgaacgtggc aaccttttat
     1201 atcgccggaa ccgagttggc gatcgagact gccggtacgg aattatcaac caatatgacc
     1261 atgatcgggt cagtcgccgg tattaccaag tgtgtatcga tggaagcttt ggacggcgca
     1321 ttgcaagaac gcttcggaaa gaaattcgtc gcatcaggtg gtaccgcttc tctagacgaa
     1381 gcgatcaaga aaaagttcgc caagaaagaa atgctcctgg caaaaaacct tgcgacggta
     1441 aaacgggcct acgaaatcgg cgcagaatgg gcggaaaaga acaagatcga gctgcgtgtc
     1501 ggcaatccgg ccgtcgctgc ctaagagaag gaatgacgtt gcatgtataa cgttgcacaa
     1561 gtcatcgatg aaaaatgcac cgccaagaag ggctgccggt tgtgcatcat gtattgtccg
     1621 gaggctaact gcttagactt gaacactacc aaaatggtag ccgaagtcat tatcgatcgt
     1681 tgcaaaggtt gcgaactctg tgtggttgtc tgcaacgccg ccaagcacga agcaatcatg
     1741 atgcaagctg tcagcgccac aggccagttg atgtcgaaaa agggagaatc tgcggctttg
     1801 ggtcaagcct accaaggcta accgagagag gttcgagttg aaaaccccat agcctcaccg
     1861 ctatggggtt ttttgttggg cctcagtgag gcacacatgg agagcgaaga caacgtcata
     1921 gatgagttac tccgagaaat ctctggtttg atcggcgaat atcctaaggc tatcgagcga
     1981 cgggcagcag ccatccaagc tacgggtaaa gaccctgagc ttgtcgaaaa gctcgtcaaa
     2041 gcagcggata ccatgcgcga tagcggcaat ctttacctca cctgggcgag acattatgct
     2101 gccctggcgg aaggcaacac cgacgcctcg tctgacgaag acgaaaccga agatttcgac
     2161 gtctaaaaaa tctccctatt cctcgaagag ttttgctaaa at
//
LOCUS       DATMTI010000654        25499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_66701, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000654 DATMTI010000000
VERSION     DATMTI010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 25499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25499
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66701"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VN039_10440"
     CDS             <1..403
                     /locus_tag="VN039_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46416.1"
                     /translation="DRLRHSSRFLEWLTTQAARLGSTAFALWSLLQSGFTSDQIEGAT
                     PLTNTTPPVITGTPKVGVVLTSDNGAWSVATGLTYAYQWDAGGAPVGTNQNTYTPVAG
                     DVGKTVTCTVTATAGASSASSTSAATAAVAA"
     gene            413..1102
                     /locus_tag="VN039_10445"
     CDS             413..1102
                     /locus_tag="VN039_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46417.1"
                     /translation="MFEKIREALTKLDPFNDAHWTTDGQPRLDTLKFFAGEDVNREQV
                     LSAAPEFTRTTAQAAVQADPAAQGDNGTGDGAAAAPVANSAAPVVPPIPPVPQESETP
                     NLGGTQMADTSASDELEGQIKDLDTRIEDLRQDIAAATTFMKGVQAERDALQKKWDAL
                     QPPEHIRHTNAVQDYLAARRAQLAERKDNKDAIKSAGLDLKQLAGRMRAPIDAVRQRS
                     TVLGVKRPPGV"
     gene            1104..1442
                     /locus_tag="VN039_10450"
     CDS             1104..1442
                     /locus_tag="VN039_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46418.1"
                     /translation="MGWLGWQLDKRRRERQNVAFFKTLLATPATGAFKLPANARLAFQ
                     SVAGAAQGTAAATLVGASTRNIACPTALVAGDYFVFDNAEIGTTITPSAGWNVLLDVG
                     LGVFKKIGAG"
     gene            1442..1990
                     /locus_tag="VN039_10455"
     CDS             1442..1990
                     /locus_tag="VN039_10455"
                     /inference="COORDINATES: protein motif:HMM:NF042800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding protein"
                     /protein_id="HWV46419.1"
                     /translation="MAFTVEDGTGLATANSFCDVPTADAYFTDRNTASNPLAAAWLAL
                     AIGDKQALLIEATDYINVRFNGKFLGQPVNPGVQALEWARFLDPTITTLPQPLPACLQ
                     KATCEYAIRAKAGPLAPDPVTDPSGYQVVRSLRRVASIQKETEFAVKGPGSIPQYLKP
                     YPAADMLLRPILLPSYGRVIRN"
     gene            1992..2378
                     /locus_tag="VN039_10460"
     CDS             1992..2378
                     /locus_tag="VN039_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46420.1"
                     /translation="MADHQEFLDLANEMILEEGRQVTLQTLNPAVSDPATPWKTVGQT
                     FNALAVVMAVFVPVTGYTKLGDEIVDLEMFKNSQQVCLIGGGDGQTEMETAVAILDEG
                     NQWKVDRVLKLRPAGLSILYAFGVSR"
     gene            complement(2549..2800)
                     /locus_tag="VN039_10465"
     CDS             complement(2549..2800)
                     /locus_tag="VN039_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46421.1"
                     /translation="MALSYVVVLEFADEIDLFEWPACAYLIDTRIAKPNQTTPSSPVR
                     LSKCLGCVIRRFRTVTDWGKYLDVKSSLTAPARLTILAR"
     gene            2799..4340
                     /locus_tag="VN039_10470"
     CDS             2799..4340
                     /locus_tag="VN039_10470"
                     /inference="COORDINATES: protein motif:HMM:NF039819.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HWV46422.1"
                     /translation="MTCISNKIDSNVTGLRFTEEECIGILRTVGSVLPPPVWYTQEPN
                     SYNNFGAKVTTVARNPINPSRQRKKGTVTDLASAGGYTQDCTAGNLVRLFQGFCFANA
                     RQKASVQPLNGTQNSVSGVTAAAKTYTVSAGGAAFISGRLVFATNFTNAGNNGLKSVA
                     SSTATTVVVNETTVDEAAPPANANLQVVGHQFAAGDLAVTMNAGSVYLNCTAADFTAM
                     GFVVGEWIFIGGDVANSAFASFSGFARIGTIAAKQLLLDKIGWSAPAADPGTGKTVRL
                     FYGTVIRNEDDPTLIVRKSYQLERSLGSDANGPQYEYVVGAVPNEFTLTLKQAEKVTM
                     DFTYVGTGYETNNGTIGPKAGTRPSSVIEDAFNTSSDFARIRMSQVVPGNPSPTPLFA
                     FATDMTLTIKNNVNANKAIGILGAMDVSAGTFEVGGASTVYFATVDAAAAVRNNADVT
                     LDIIMVKKNAGLLFDIPLLTLGNGEATVAQDQPIMLALDTNAAQSTFGNTLLFMHFPY
                     LPNLA"
     gene            4354..4773
                     /locus_tag="VN039_10475"
     CDS             4354..4773
                     /locus_tag="VN039_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46423.1"
                     /translation="MNLYEQFETNSQCETDGIFIDYGPNSKGKNIRFKLARAGGKNIQ
                     FAKAMEKYSKPHRRMIELGLLAPSVAEGIMRTVFIESVLKGWENVEDRAGAELPYSPE
                     AADALFTELPDLFTNLNELARDASNFREELLKADVKN"
     gene            5143..5571
                     /locus_tag="VN039_10480"
     CDS             5143..5571
                     /locus_tag="VN039_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46424.1"
                     /translation="MSLSQFARWIGGIGQNVDPLTTQIVKEMVHEMAPVLVKATPVDT
                     GRARSNWQGAVNNIPQGILYYPAPLAPSSASDGTAEGIQSINDAANAYKGRGYIAIAN
                     NVPYIGVLNTGSSSQAPAGFVEDAVLAGIMSLNGKSILVP"
     gene            5571..8462
                     /locus_tag="VN039_10485"
     CDS             5571..8462
                     /locus_tag="VN039_10485"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HWV46425.1"
                     /translation="MATQTQNIVITVTQTGAQAAGQAISGVGTSAQKSSDSVKMLQDA
                     LKGLGALLVVDQLKEWVDVWVRATGLVNIFSKSQAETNLVLQKLYDISQNTRQSLEAV
                     VKTYHQMAIAGQQLGATQNDILKVSQLVNQAFAIQGTPANVAKAGILQLSHAFSEGIV
                     RGRQFNSLLIEMPRVLQIAANYIEGAGGSVGKLRQMMLAGKLTSADLFQALVKGSQDL
                     DGQFQKTGKTFAQAFIVMENALIRFFGQMSQASGFSQGFYNIMKLLADNIDTVVKSLL
                     AMASPFILQGLLGIVNAFKAMAVAAAANPIGAIITALTVATTAAILFKDQIILNQDEQ
                     LTLGDYISAVGGLIKDVFGDVGPQHVNVFKAAILGLEDAIHTVSRDFLDLKARIMGAS
                     AVDANADAQSRQEQFNRLLQQKIDLEQHIALIEGKPAPIRWLMTSELEETKSKLQDTI
                     GMMMKAQDEQKKLLTGQSVGAPKPKGTAGDGLIADLTKRAKARKEERQKELADELKQQ
                     KDAVAELNKPLGETQDFIGEGKGKKAPKNPLDNPYKLQTAYTALLNKIQPLSGAVQEY
                     ARAQDILDREQKAGNITAERKAQLLELVKNHYRDIVDPIGAVTRQMDRENAYLLQNSE
                     ARQVSQKQYQIEENLRKQGIFLSQQETEQLKAQIVANQELARVTQQKDAIFSSVMQQQ
                     RQYIDLLKAADQLEKNKDLTKGQTDQYLLTKGPNADLFAGTKEAIEANKQQFTDMYSY
                     IDSLQERQTISSQTASIARVRVFAQEQATVMQGYSNFFGQLAQLRTADGKKGSKIAKA
                     AAIAQASINAYTSATGAYASASAIPIIGWVLGPIAAAAALAAGLANVAQIRSQSAGFM
                     MGGYTGNMGAMDEAGPVHGREYVMDQMTTSRIGVHNLDDLRSGQAHVVRGGEQNQAPV
                     VNVAPAQLSVAVVSSREEAMNALRSEEGQAFIVETMAKNSQTLTRLAGG"
     gene            8466..9419
                     /locus_tag="VN039_10490"
     CDS             8466..9419
                     /locus_tag="VN039_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46426.1"
                     /translation="MAYDIATLTLAGGGGVSCHYQMLAKIKALAESVGWVTQRYDTTS
                     SSNYEWIGKAPGLSGAEQIYAGFRTYQDSGADYYNLLAMVATGYLPGNTFDSQPGAIS
                     SGVPSHNNAITYFMTANAQRIALCMKVGTPVYEHAYVGKFFPYARPGEFPNPLFCAGM
                     LNGAAAVRYSEATHTNGIMGYQPGGWGATRYGYSRDNNGAWGVRRHLPFNNRDSYNIC
                     NISRPADLVVAQAVYGLEPIIPYDVGTNVWGELDGVYGITGFNSGSENVVQLGGTTVD
                     QTGMTVKQAVDAIIAAGGRAFVVLQDVARTTFGSYIALEMK"
     gene            9419..10312
                     /locus_tag="VN039_10495"
     CDS             9419..10312
                     /locus_tag="VN039_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46427.1"
                     /translation="MAGYQSGSATSFADLQAAILTFLQTQGWTLTNTVLNKGTGFFQF
                     TNTASKLVLNTGTGQSGTALTGVPASQVCMLSADNPVGFPVTYEFHYFNSPDEFICVI
                     QHNGVFYQQLALGQSLSANIGGTGWWMEGSYDNRYLSTNKGYSSDSSGANASGSWQYQ
                     GFQFFAMGQYGPRGMWLNYGVDGTNAWGAEANLNSGWVNWMMMQASPNQLNQGTILVP
                     IRVMASRPSSLYTEVLTVSNIRMCRNDAFDPGAIYTLGTDKWKIYPFYSKNTAQRNGV
                     AWGTGAFHSGTYAYAVRYQGP"
     gene            10314..12029
                     /locus_tag="VN039_10500"
     CDS             10314..12029
                     /locus_tag="VN039_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46428.1"
                     /translation="MTALLGKQAASPLYGGANPAVSSDMNGYTTPYAYTPYPWLGSGA
                     LKANAPAGTQRIPPPNTWAIIGKASPAFMQDFYYRVLVSPSTLALGTVASSQTRDVVV
                     WNAYPDQIANLSSISISDPVGIAVTGQAAPYSMSALQELKYTVGISTSGAANIDAYVS
                     FNFSNVASPLPVHITGSRAIKFDIMPEVPVQEDWEWKSDVEVAIDGTEQRIALRGEMP
                     RITQTVKVLFDNNPDVRRMISQLMSSVGRLWIPQFQYAVKVTQATATGGNRLYFDPTR
                     TDIRVGEYLMIQTPDGTTALMGVSALNADGVTLSGTLALDIPLRSIIMPGVSSVLPDA
                     QAVTRYAVDSVGTVGLVCRMLWSRTSLIRPGAAAVVLPTFLGYPILEKRPSADSDIKE
                     QAYTGQQDVDNETGPLDIVSRWVYTRWGGDRSFLVNRIADPTVMDYWKTFLAYTRGQC
                     KVFWMPSFRQDFIPMSVVAPGANSFAIDGTDYAENLFPLLTHKYLRITTAAGTQDVTV
                     TQAGSQNGLSFLTFSPALPTGAGWNNVQFLSLMLPCRLTDDKVTWKHSGLSSTLDMTV
                     RTAEV"
     gene            12026..12859
                     /locus_tag="VN039_10505"
     CDS             12026..12859
                     /locus_tag="VN039_10505"
                     /inference="COORDINATES: protein motif:HMM:NF020913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage BR0599 family protein"
                     /protein_id="HWV46429.1"
                     /translation="MTYRDQENSQSGGAPFELYEWIGTYASYYYTSDLFPHIFNGITY
                     QPLAGLTRGSIKAGTHAEDNTDLTVTLPITSQLAQDYAFQVTPPSLQLNIYRFQRDAT
                     LSTDYEVAWSGPVNSITVANEKASFIVPSMFGNLLRGAVPNVYIQPPCNNTLFDAQCK
                     VSRAANSVATTVTANAGLTLTVGPLTAFPPGWFIGGEVVASGTNERRMIINQAGNVLT
                     LNYSFGKLAIGAQVQVSSGCDHSYTGANGCPKYNNQVNFGGCPFVPGETNDPYAKGLQ
                     P"
     gene            12856..15348
                     /locus_tag="VN039_10510"
     CDS             12856..15348
                     /locus_tag="VN039_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWV46430.1"
                     /translation="MNWASIILGPLTVKILDWLFKTPAQADATAAKFGDFNIPRSKYG
                     DPAPLLYGTARQKSPLTLWFGDYNPVPIKKKQSTGLFSSKRVIVGYKNYIGMDMLLSI
                     GPGHVLKQIWVDNTSIWTGNLTTDSSININKPNQFGGDDQRGGYVGKMSFYTGGSSPV
                     QNAYLKKILGPNVPAYNGFCRVVMEAFYIGTSAQVPVPSFEMSRLTNGLSASYSIMPN
                     GLDLNPLEIAYDAFTQQYGRFGLPVAVIDLPSWLAAAQTLYNEGMGMTFLIQSAITGK
                     DLLNELMDVSDGFMYQEPSNRKMVVKLVRADYNVATLDTLDQSIVSDISNFGKNTWDQ
                     TFNQCRVTFADRANSYDDSIAMQTDFANINFQQKVVSTNLTSAGIRDATVANKYAASQ
                     LSRANVPLYTATLKCNRKAAKYRPGDVVLLNWSPFGLTGMVMRIGKVELGELVKGVVT
                     LTVAQDRYSTSTPIFAAPPPTYWPPVTFQPAPVTVRKIIELPYFFGSVAQPPGATDGI
                     LQALASPPASASISYRADMTANADTTFANATTVMEDAPYNGSGTLRDAYAALTGRSTG
                     QDAVGFAVNLWLPTSAVPPSPGAFDNTGQTLLVIDNEIIGYTGCVVNPDGSYQFSGIR
                     RALLDTDFADHSAGAQVYFLSGQDDQFNAYINNTSTQRIRLIDKAATATLDPSLALVD
                     VFTLSQRTYKPAPPDYVQLNGSRTPISQYVSAAIAVTWRERNRKTATLVQYNDPTEVA
                     EPGTDYGIRYRVNAGAWSARTFVTGVSYTIPALGNSYAGTVDVEVVSRRDGVISNVGD
                     ICTIVLTIETFHLMTSANELLTSSGGDYLRTM"
     gene            15348..16097
                     /locus_tag="VN039_10515"
     CDS             15348..16097
                     /locus_tag="VN039_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46431.1"
                     /translation="MADQILNLNGKQAAAGSGVINIVPADYISGLKMVWVSATALTVT
                     TGAACRPSDSLLMAPAANIAKTALALTASTWYHVYLYMNGANPDIEIVTTAPAAPYSG
                     TACAKTGDTSRRYIGSVLTDASGNIIRFDQTGNRIDYMMSINNAPLHPLNYAGATITT
                     PVSLAAAIPVTARRAAMYLENSTTNGSIAYISVPPLASLADVITFVRPGGRLSALYVV
                     DSTQSFNYAFNGAPAAGTGLDAWVTGYVYER"
     gene            complement(16161..16832)
                     /locus_tag="VN039_10520"
     CDS             complement(16161..16832)
                     /locus_tag="VN039_10520"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HWV46432.1"
                     /translation="MDDKARILEKIKKCLALGKSSNAHEAAAALRQAQKLMDKHGVSQ
                     EEIDALGYGFEEVEIPVQTNVKLPAYISYFVALMKQAFGVRPIMSSTLRQSDYSYSVK
                     YFGPTGRVITAAYAHQVVWRAMNRDWNAHLKANPEWKGKKGARSGFFIGWIDEVWSKV
                     MAIGFTDEEAARTELAVRSTGLKVGPTTTSELKVYNATGAAGREAGSGFQIYRGTGTE
                     RNRLR"
     gene            complement(16822..17037)
                     /locus_tag="VN039_10525"
     CDS             complement(16822..17037)
                     /locus_tag="VN039_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46433.1"
                     /translation="MPTHKSRAITIFLFVILVLLYVFLPEDKSVEQDARQYCQMVHDH
                     SWPDFNRVYHAQCNADGTLNMDYINGR"
     gene            complement(17208..17573)
                     /locus_tag="VN039_10530"
     CDS             complement(17208..17573)
                     /locus_tag="VN039_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46434.1"
                     /translation="MKDAQLAIEMLHSAKRNIKSAQRSEALMKHVRPYSPQYIGLAHA
                     AQRELLTARMLLQAARRVHPATTPKGKGSARPVAGKAGRAAIPVLCGCAPYPARAPVV
                     WANDLNFGYGPDFDHVPVN"
     gene            complement(17570..17851)
                     /locus_tag="VN039_10535"
     CDS             complement(17570..17851)
                     /locus_tag="VN039_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46435.1"
                     /translation="MSLVTVTFEMSNGSILKCEYGYTSSPATFWEPEDVNVGDPTYYI
                     DDDEISYDDLPKGLDKIADALYEADARSAKFSYRESEYERDYDFEDYDR"
     gene            complement(17915..19942)
                     /locus_tag="VN039_10540"
     CDS             complement(17915..19942)
                     /locus_tag="VN039_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46436.1"
                     /translation="MRRDAIGMFWRDEPVVKVVKEKVKVTPPDPVWLNDDYLPYLSEA
                     RAFNFPQFSDAELVEAMGCNLTLDCEVYSNYFLCAFKCPDTKRVTYIETVDGALTELD
                     RSKLLWILENFQTISFNGLSYDLTMLALALAGKTTQEIKQASNELIEQEISPYQILKE
                     RRVKKLKLNHIDLKEVAPLHGGLKAYSGRLFCRRMQDLPFAPDKWLSDDQICVTRYYC
                     VNDLDNTELLWLTLQEQIQLRIDVGRMYGLDLRSRSDAQIAEDVFSLEVEQMSGRRAF
                     KPIVAPGTMYFYKIPSYLNFRTELLRWVLNEVRIAPFLVAFHGAVEEPKQFEKLWDLQ
                     IADSKYAMGIGGLHSKEKKIAHHTDSEYTLHDFDVTSFYPRIMINQGLFPHHLGPNFL
                     RAFNAKVEERIVAKRIKDMVKANGFKILVNGSFGKLGSKYSNLYAPDLLVQVTMTGQL
                     SLLYLIEKLELAGIHVVSANTDGIVVKCKRTMHSVMYSIVKEWERETCFELEETRYMS
                     LYSRDVNNYIAVKQKFDKENKVWIEQPDKCKTKGAYANPWNDASDKSMWMHKNPVNVI
                     CIDAIEAMLTKGVPIVTTITKCRDITKFLTVRKVSGGAVKDGEYFGSHIRWYYSNEVE
                     GPLIYGKSGKRVPRSMGARPMMTLTEEFPQDVDFDWYVKESEKILKQIGYV"
     gene            complement(19939..20898)
                     /locus_tag="VN039_10545"
     CDS             complement(19939..20898)
                     /locus_tag="VN039_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46437.1"
                     /translation="MFKELKFVQGAVAKKDFIPAMTHFQIKDGRVTAFNGTLALSSPI
                     SCDLDCKPKAGPMVQAIARCEDTIQLSLTAAGRLSVRSGKFKAFIECTEDDSPHAEPS
                     GDEVSFDGALLLRAFQELQPFVGNDASRPFTNGILIRNGSAYATNNVCLAQFYMGFAL
                     PSVVNLPRSAVAEILRINQAPVRMRVESNSVTFEYEDGRWLRTQLFSTEWPDVDSILN
                     RDHGATPLSEELFKALEAVKPFCDKNGRVILEGCTVRTHDSLEEGSSYTLHENAAWPL
                     SSYRVEMLSLLEGVEFFDAQSFPAPGLFFKGDRMRGAIIGLRL"
     gene            complement(20898..21119)
                     /locus_tag="VN039_10550"
     CDS             complement(20898..21119)
                     /locus_tag="VN039_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46438.1"
                     /translation="MKKELLLSAVAEMLPSDILCKRIVVQYYSEQPGDYGELRKRISK
                     IQDSCDLSKNKIDVSYDFKNCVVTLEITP"
     gene            complement(21119..22039)
                     /locus_tag="VN039_10555"
     CDS             complement(21119..22039)
                     /locus_tag="VN039_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46439.1"
                     /translation="MNIFMAAVYSNSYMPGQNRYVKLNDHERRLVEEIPHILESWHYV
                     GKQKFVDEMRADSAQIFLDSGAFSAYTLGVTLSVEEYCNYIQRNSDIIRKEDGVVMAS
                     VLDGIGDPLQTYRNQLEMEARGVRPLPCFHSGEDSRYLDYYVANYPYITLGGMVGAST
                     EQLMRWLDRIWDKHLVDGAGKPKLRVHGFGITSIPLMERYPWYSCDSSSWIQSAAFGS
                     IVSPHHGPLNVSEKSPSRHDAGQHVTTLTQIECDYVFKMLEQQGFTYERLSTVYESRA
                     AYNLWAYGVINTMINAHHSPRFRDMVQELF"
     gene            complement(22169..22783)
                     /gene="folE"
                     /locus_tag="VN039_10560"
     CDS             complement(22169..22783)
                     /gene="folE"
                     /locus_tag="VN039_10560"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWV46440.1"
                     /translation="MSASDRVITRNLFPHELTKEDLIRQLLLRVETVPEGYGLRQGLE
                     DTPKRVSKAFDHWFGGYNIDIAEVLKTQFEDGGEGYDQFVIVKNVPFYSKCEHHMADI
                     FGTATIAYIPSNGKVVGLSKLSRVLDAYARRLQVQERLTKQVADALVEHLQPLGVGVV
                     IKARHMCMESRGVCQQGHHTITSALRGVIRDEVATRAEFFALAS"
     gene            complement(22770..23225)
                     /locus_tag="VN039_10565"
     CDS             complement(22770..23225)
                     /locus_tag="VN039_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWV46441.1"
                     /translation="MSGKHLSYKNFGNEQGWSCCFRQWKAKHSHCSLLHGYALGVKFE
                     FMSDCLDDKNWVMDFGGFKEVKKWLENLLDHTMLVSEDDPFKDDICMLAGIGIANVIV
                     LPNVGCEALAQYIHEWVARWTKIQTQGRVDLYKTTVYEHGANGASYVSL"
     gene            complement(23215..24636)
                     /gene="queC"
                     /locus_tag="VN039_10570"
     CDS             complement(23215..24636)
                     /gene="queC"
                     /locus_tag="VN039_10570"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWV46442.1"
                     /translation="MCAIFGALLRNVQTKHDLETVNKILAAIMLSSEERGRDGWGYQC
                     HADGKMWGDRAVPQHTTTDHFGKPMVPPYDGEYFRRTFMFGNIIGNMRAEPTTEWVKE
                     KASSDQQPYIAGAWSIVHNGTIANDAELRTHRLRTQIDSAAIAELLEGYKPDDRYSID
                     QVLAGFGNAMEMLVGSYAVLATAAGYDRAVLVSCNYRPVWYITTPWGFFFASSRDYFP
                     QGFVPEMVEPYTHAIFYVGQNGQLGCRKMAHRSQGASFKALVIASGGLDSTVAAVEMI
                     RRGYEVELIHFEYGSRAQGPEVAAVTAIAARLGVTLTRFPLPVYKPADSPLLRESNGV
                     AKGESGAEFAHEWVPARNLILMSVATAYAEANGFDTIVLGNNLEEAGAYPDNEPEFVR
                     RFSDLLPFAVGVNKRMRVEMPVGNLMKHEIVAMGLEIGAPLDLTWSCYHAHDKHCGDC
                     GPCFMRKTAFEINNQKEVIEYER"
     gene            complement(24639..>25499)
                     /locus_tag="VN039_10575"
     CDS             complement(24639..>25499)
                     /locus_tag="VN039_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HWV46443.1"
                     /translation="GPGPADLRAPGAVSCYACQWVTPHRARNAPCATYPGADMQLNTQ
                     KPEKRVESDGRELSVHSIFHTIQGEGPFCGTPAVFVRLAGCNLQCPQCDTNYTTGRKM
                     MKTHEILREVLNLTEGLNKTRLVVITGGEPFRQELSNLFDLLFCTEFFVQVESNGTLA
                     PPEEPENTDLDHWFYNHNAWERNGVYIVCSPKAGKVHPKIHQHCCAYKYVGGRHNLSK
                     EDGLPLRPLEHPSAKGVPRPKGDKDIYLQPTDEQDETRNAFNLKACVESCLKHGYILQ
                     LQIHKLIGVA"
BASE COUNT         5580 a         7233 c         7196 g         5490 t
ORIGIN      
        1 cgaccgactg cggcactcct cgcgctttct ggaatggctg accacgcaag ctgcgcgtct
       61 cggctcgact gccttcgcac tgtggtcatt gctgcagagc ggcttcacct ccgatcagat
      121 cgaaggcgcc actccgctca cgaacacgac tccgccggtc atcactggca cgccgaaggt
      181 cggcgtcgtc ctgacttccg acaatggcgc ctggtctgtc gcaactggcc tgacttacgc
      241 gtaccagtgg gacgcgggcg gtgccccggt cggcacgaac cagaacacct acacccccgt
      301 ggcaggcgac gtcggcaaga cggtgacctg tactgtcacg gccaccgctg gagcgtcttc
      361 cgcttccagc acgtccgctg caaccgctgc cgtcgcggcg taaggagaag tcatgttcga
      421 gaaaattcgt gaggcgttga cgaagctgga tccgttcaac gatgcgcact ggacgacgga
      481 cggtcagccc cgcctcgata ccctgaagtt cttcgcaggc gaggatgtca atcgcgagca
      541 ggtgctttcc gcggcacccg agttcacccg tacaaccgcg caggcagcgg tacaggccga
      601 tcccgcggct cagggtgaca acggtactgg cgacggtgcg gccgcggccc ctgtggcgaa
      661 cagcgcggcc cctgtcgtac cgcccattcc cccggttccg caggaaagcg agaccccgaa
      721 tctcggtggc acgcagatgg cggatacgtc cgcctcagac gagctcgaag gccagatcaa
      781 ggatctcgat acccggatcg aagacctgcg ccaggatatc gcggcagcga cgactttcat
      841 gaagggcgtc caggccgagc gcgatgcgct ccagaagaaa tgggacgcct tgcagccgcc
      901 cgaacacatc cgacacacca acgcggtcca ggactacctc gccgcgcggc gtgcgcagct
      961 ggccgaacgc aaggacaaca aggacgccat caagagcgcc ggcctggacc tgaagcagct
     1021 ggcaggcagg atgcgcgcgc cgatcgatgc cgtgcgtcag cgttccactg tgctgggcgt
     1081 gaagcgcccg cctggagtgt gagatgggtt ggcttggctg gcagcttgac aagaggcgtc
     1141 gagagcgaca gaacgtcgca ttcttcaaga cgctccttgc gactcccgca acgggagcgt
     1201 tcaagctgcc agccaacgcc cgccttgcgt ttcagagcgt agctggcgca gcgcagggca
     1261 ccgcagctgc gacgctggta ggcgcatcta cccgaaacat tgcctgtccg acagcactcg
     1321 ttgcaggcga ctatttcgtg tttgacaatg cggagatcgg aacgacgatc acgccatcgg
     1381 cgggttggaa cgtccttctg gatgtcggcc tcggagtctt caagaagatc ggagcaggct
     1441 gatggctttc acagtcgaag atggaactgg gcttgccacg gcgaacagct tctgcgatgt
     1501 cccgactgcg gacgcgtatt tcacggatag gaacaccgca tccaacccgc tcgctgctgc
     1561 gtggctcgca ctggccatcg gggacaaaca agcgctcttg atcgaggcga cggattacat
     1621 caacgttcgc ttcaatggga agttcctcgg acaacctgtc aatcctggtg tgcaggcgct
     1681 ggaatgggcg cgcttcctgg atccgacgat cacaaccctc ccgcagcctc ttccggcttg
     1741 ccttcagaag gcgacgtgcg aatatgcgat ccgtgccaag gctggtccgc ttgcccctga
     1801 tcctgttacg gatccgtcgg ggtatcaagt tgtccgttcc cttcgtcgtg ttgcgtcaat
     1861 ccaaaaggaa acggagtttg cggtaaaggg tccgggctcc attccgcagt acctgaagcc
     1921 ctacccggcc gcggacatgc ttcttcggcc catccttctt ccgtcttacg gacgtgtcat
     1981 aaggaattga catggcagat catcaagagt ttctggatct ggcaaacgag atgattctgg
     2041 aggagggccg acaggtcacc ttgcagacgc tcaaccctgc cgtctccgat ccggccacgc
     2101 cgtggaagac agtcggacaa acattcaacg cccttgctgt ggtcatggcg gtgtttgtcc
     2161 ctgtgacagg ctataccaag ctcggcgacg aaatcgttga cctcgagatg ttcaagaatt
     2221 ctcaacaggt gtgcctcatt ggcggtgggg acgggcagac cgagatggaa acagctgtcg
     2281 ctatcctgga cgaaggcaat caatggaagg tggaccgggt cttgaagttg aggcctgccg
     2341 gactctccat cctctacgct tttggcgtct cgcgatgatt acgtatgatc aagcgcgaga
     2401 tctcatcctt gcacctttca agacgatatg ggtcgacagc ctgaactatc cggcacagta
     2461 cactgaccgg gatggaggac tgcctcctac tacccaaacc ccatgggcgc gtgctatagt
     2521 gcggcacgtg aatgggaatc aagcttccct agcgtgcgag aatggtcaat cgcgctgggg
     2581 cagtcaagga actcttcaca tccaagtatt tgccccaatc ggtgacggtt cgaaagcggc
     2641 gtatgacgca gcccaagcac ttgctcaggc gtaccgggga actcggagtg gtctggtttg
     2701 gtttcgcaat ccgcgtatca atgaggtagg cgcaagcggg ccattcgaac agatcaattt
     2761 cgtctgcgaa ttccaatacg acgacatagg ataaagccat gacctgcatc tcgaacaaga
     2821 tcgactccaa cgtcaccggc ctccggttca ccgaggaaga gtgcatcggc attctgcgca
     2881 ctgtcgggtc ggttctgccc ccgccggtct ggtacacgca ggagccgaac tcctacaaca
     2941 acttcggtgc gaaggtgacc acggttgctc gcaacccgat caacccgagc cgtcagcgca
     3001 agaagggaac cgtcaccgac ctcgcgtcgg cgggcggcta tacccaggac tgcaccgccg
     3061 gcaacctcgt tcgcctgttc cagggcttct gcttcgccaa cgccaggcaa aaggcgtccg
     3121 tgcagcccct gaacggcacg cagaattccg tcagcggtgt caccgcagca gcgaagacct
     3181 acacggtctc tgcaggcggt gcagcgttca tctccggacg cctggtgttc gcgaccaact
     3241 tcacgaacgc cggcaacaac ggactcaaga gcgtcgcgag ttcgacggcc actaccgttg
     3301 tggtcaacga gaccacggtg gacgaggctg cgccccctgc caacgcgaac ctccaggtcg
     3361 tgggacacca gttcgcggcc ggcgatcttg ccgtcacgat gaacgcaggt tcggtctacc
     3421 tgaactgcac tgcggcggac ttcaccgcga tgggcttcgt ggtgggcgag tggatcttca
     3481 tcggcggcga cgttgcgaac tctgcattcg catccttcag cgggttcgcg cgtatcggca
     3541 ccatcgcagc gaagcagttg ctcctcgaca agatcggctg gagcgcaccc gcggcggatc
     3601 cgggcaccgg caagaccgtg cgcttgttct atggcacggt gatccgcaac gaggacgacc
     3661 cgacgctgat cgtgcgcaag agctatcagc tcgagcgttc gctgggttcc gatgccaacg
     3721 gtccgcagta cgaatacgtc gtcggtgcgg tcccgaacga gttcaccctg accctcaagc
     3781 aagccgagaa ggtcacgatg gacttcacgt atgtcggcac cggctacgag acgaacaacg
     3841 gcacgatcgg tccgaaggcg ggaacccgcc cgagcagtgt gatcgaggat gcgttcaaca
     3901 cgtcgtccga cttcgctcgc attcgcatgt cgcaggtggt gccgggcaat ccgagtccga
     3961 ccccgctctt cgccttcgcg accgacatga ccctgacgat caagaacaac gtcaatgcga
     4021 acaaggctat cggtatcctg ggcgcaatgg acgtgtctgc gggtacgttc gaggtcggcg
     4081 gtgccagcac ggtctacttc gcaacggtgg atgccgctgc ggccgtgcgc aacaacgccg
     4141 acgtcacgct cgatatcatc atggtcaaga aaaacgctgg gttgctgttc gacatccccc
     4201 tgctcacgct gggcaatggc gaagcgaccg tggcgcagga tcagccgatc atgctggccc
     4261 tggacaccaa cgcggcgcag agcacgttcg ggaacactct cctgttcatg cacttcccgt
     4321 acctcccgaa ccttgcataa caaggaaaac ttcatgaacc tgtacgaaca gttcgaaacc
     4381 aattcgcagt gcgagaccga cggcatcttc atcgactacg gtccgaactc gaagggcaag
     4441 aacatccgct tcaagctggc gcgcgctggc ggcaagaaca tccagttcgc gaaggcgatg
     4501 gagaagtaca gcaagccgca tcgccgcatg atcgaactcg gtctcctggc tccgtcggtg
     4561 gccgagggca tcatgcgcac ggtgttcatc gagtccgtcc tcaagggctg ggagaacgtc
     4621 gaagaccgtg ccggtgcaga gctgccgtac agccccgagg ccgcggatgc cttgttcacc
     4681 gaactgcccg acttgttcac caacctcaac gagctggcac gcgacgcgtc caacttccgc
     4741 gaggagctgc tgaaggccga cgtaaaaaac tgatcgaggt ccttctctat aacgtcgagc
     4801 aagggccggt ggagcggacg ttagcggaac aagccattcg caatcacatg ccgctacctc
     4861 cgaagatcgc gaatgcccct gccctattgc aggggctttc gtttttctat atagtgttca
     4921 tggacttgac cacgacaagg cccttgacag atatgtcgga aggacctatc agctggctta
     4981 caattgacgc gtattgcagg caccatcaga tcactggaat tcaacgggag gatgtagagt
     5041 acgttatccc tcaaatggat caagcctaca ttgcgaaatg cgtcgagttg agaaagatcg
     5101 ctcgagccaa caaaccccag ccttcaaggt agagagatga gcttgagcct ttcacaattt
     5161 gctcggtgga tcggcggaat cggacagaac gtcgatccac tgacgacaca gattgtgaaa
     5221 gagatggttc acgagatggc tcccgtcctt gtcaaggcga cccctgtgga tacggggcgc
     5281 gcgcgttcca actggcaggg tgcggtgaac aacatcccgc aaggcatcct ctattaccct
     5341 gcaccgctcg caccttcaag cgcgagcgac ggaacagcgg aaggcatcca atcgatcaac
     5401 gatgcggcca acgcatacaa gggccgcggt tatatcgcga tcgccaacaa tgttccctat
     5461 atcggagttc tgaacacagg ttcttcaagc caagcgcctg ccgggtttgt tgaggatgct
     5521 gtgctcgctg gcatcatgtc cctcaatgga aagtcaattc tggtgccctg atggctactc
     5581 aaactcaaaa cattgtcatc acggttacgc agactggcgc gcaggctgcg ggacaagcaa
     5641 tctcgggcgt gggcacttcc gctcagaaga gtagtgactc cgtcaagatg ctgcaagacg
     5701 ctctcaaagg gcttggagcg ctccttgtgg ttgaccagtt gaaggaatgg gtcgacgtat
     5761 gggttcgtgc gacgggtctt gtcaacatct tctccaagtc gcaagctgag acgaatctcg
     5821 tccttcagaa gctctacgat atctcgcaga atacccgcca gagcttggaa gctgtggtca
     5881 aaacgtatca ccagatggcc atcgcgggcc agcagctcgg agctacgcag aacgatatcc
     5941 tgaaggtgtc gcagctggtg aaccaagcct ttgcgatcca gggcacaccg gccaacgttg
     6001 cgaaggcggg tattttgcag ctgagtcacg cgttctcgga aggcatcgtt cgaggcaggc
     6061 agttcaacag cttgcttatc gagatgcctc gtgtgctgca gatcgctgcg aactatatcg
     6121 agggagcagg tggctctgta ggtaagcttc gccagatgat gttggcgggc aagctgactt
     6181 ccgcagacct cttccaggcc ctcgtgaagg gctcgcaaga cttggatggt cagttccaga
     6241 agacaggcaa gacgttcgcg caggcgttca tcgtcatgga gaacgcactt atccgattct
     6301 tcgggcagat gtcgcaagca tccggcttca gccagggctt ctacaatatc atgaagcttc
     6361 tggccgacaa tatcgatacg gtagtcaagt ccttgcttgc catggcatcg cctttcattc
     6421 tgcaaggctt gctcggcata gtcaacgcat tcaaagccat ggccgtagcg gctgcggcga
     6481 atccgatcgg cgctatcatt accgcgctca cggtcgcgac gaccgcagca attctgttca
     6541 aggaccagat tatcctcaat caggacgagc agcttacgct cggtgactac atctcggccg
     6601 tcggtggtct tatcaaggat gtgtttggtg acgtcggccc gcagcatgtg aacgtgttca
     6661 aggcggcgat cttgggactg gaagatgcca ttcatacggt ctctcgagac ttcctggatt
     6721 tgaaagccag gatcatgggc gcgagtgctg tcgatgcgaa cgcagatgca cagagcaggc
     6781 aggagcagtt caaccgcttg ctccaacaga agatcgacct tgagcagcat atcgctctga
     6841 tcgaaggaaa gcccgcgccg atccgttggc ttatgacgag cgagctggaa gagaccaaat
     6901 ccaagctcca ggacaccatt gggatgatga tgaaggctca ggacgagcaa aagaagctgc
     6961 tcaccgggca aagcgtgggc gctcccaagc cgaaggggac tgcaggagac gggcttattg
     7021 cggacctcac gaagcgcgca aaggctcgaa aggaagagcg tcagaaggaa cttgctgacg
     7081 aattgaagca gcagaaggat gccgtggcgg agctcaacaa gcccctcggc gagacgcagg
     7141 acttcatcgg agaaggtaag ggcaagaagg cgccgaagaa cccgctcgac aacccgtaca
     7201 agctccagac cgcatatacc gcgctcctga acaagatcca gcccctgtct ggtgcagttc
     7261 aggagtacgc tcgcgcgcag gatattctcg atcgcgagca gaaagctgga aacatcacag
     7321 ctgagcgtaa agctcagctt ctcgagctcg tgaagaatca ttaccgggat atcgtggacc
     7381 ccatcggcgc agtcacgcgt caaatggacc gcgagaacgc ttatctgctt cagaattccg
     7441 aagcgcgtca agtatcccag aaacagtatc agatcgagga gaacctccgc aagcagggca
     7501 ttttcctgag ccagcaggag accgaacagc tgaaggcaca gattgttgcg aaccaagagc
     7561 tggcgcgtgt cactcagcag aaggacgcga tcttctctag tgtgatgcaa cagcagaggc
     7621 aatacattga tcttctcaaa gctgcggatc agctcgagaa gaacaaggat ctgactaagg
     7681 gacagacgga tcagtatctg ctcacgaagg gtccgaacgc tgacttgttt gctggcacga
     7741 aagaagccat cgaagcgaac aagcaacagt tcacggatat gtatagctac atcgactccc
     7801 ttcaggaaag gcagacgatc tcttcgcaga ccgccagcat tgctcgcgtg cgcgtgtttg
     7861 cacaagagca ggccacggtc atgcagggct attcgaactt cttcggacag ctcgcgcagc
     7921 tacgcaccgc cgacgggaag aagggaagca agatcgccaa agcagcggct attgcgcagg
     7981 cctcgatcaa tgcgtatact tccgccacgg gtgcatatgc gtcagcctca gctatcccga
     8041 tcattggttg ggtacttggc cccattgcag cagccgcagc actagcggcg ggtcttgcaa
     8101 acgttgcaca gatccggagt cagtcagcag gcttcatgat gggtggttac acgggcaaca
     8161 tgggcgcgat ggacgaagct gggcctgtgc acgggcgcga gtacgtgatg gaccaaatga
     8221 ccacaagccg catcggagtt cacaatctgg atgatctgcg tagtgggcaa gcgcacgtcg
     8281 tccgcggggg cgagcagaat caagcgcctg tggtcaacgt tgcaccggct cagttgagtg
     8341 tcgctgtggt ctcctcgcgc gaagaggcga tgaatgccct gcgcagcgaa gaaggacaag
     8401 cgttcatcgt tgaaaccatg gcgaagaata gccagaccct tacccgtctg gctggaggct
     8461 aagaaatggc atatgatatc gcaactctga cgttggcagg aggtggaggg gtaagctgcc
     8521 attaccagat gctcgccaag atcaaggcgc tggcggagtc tgtgggctgg gtcacgcagc
     8581 ggtacgacac cacgagcagt tcgaattacg aatggatcgg taaggcgccg ggtctttctg
     8641 gcgccgagca gatctacgcg ggtttccgta cctatcagga ttcgggtgcg gattactaca
     8701 accttttggc gatggtggcc acgggctatc ttcccggcaa tacattcgac agtcagcctg
     8761 gcgcaatctc aagcggtgtt ccgtcacaca acaacgcgat tacgtatttc atgactgcga
     8821 atgcacagcg cattgcgttg tgcatgaagg tcggaacgcc tgtctatgag catgcgtatg
     8881 tcgggaagtt ctttccctac gcgcgtcctg gcgaattccc gaatcctctg ttttgcgctg
     8941 gtatgttgaa cggtgcagcg gccgttcgat acagcgaagc cacacacacg aatggaatca
     9001 tgggatatca gccgggaggc tggggagcta ctcgctacgg ctacagtcga gacaataatg
     9061 gcgcgtgggg agtaaggcgc catcttcctt tcaacaatcg agacagctat aacatctgca
     9121 atatctcacg accggcagat cttgtagttg cgcaggcggt ctacgggctg gagcccatta
     9181 tcccgtatga cgtaggcacg aacgtctggg gcgagcttga cggtgtctac ggcatcaccg
     9241 gattcaacag tggctccgag aacgttgtgc agctcggcgg aacgacggtg gaccagaccg
     9301 gaatgacggt taagcaagct gtcgacgcaa tcattgctgc gggaggtcga gcgtttgttg
     9361 ttctacaaga tgtggctcgc acaactttcg gaagctatat cgccctggag atgaaataat
     9421 ggccggttat cagtcgggca gtgcgacgag ctttgcggat ctgcaagcgg ccattctgac
     9481 cttcttgcag acgcagggat ggacgctcac caacacagtt ctgaacaaag ggaccggatt
     9541 tttccagttc acaaatacag cttcgaagct tgtgcttaac acaggcacag gccaatctgg
     9601 cacagcactg accggagttc ctgcttcgca ggtctgcatg ttgtccgcgg ataaccccgt
     9661 tggctttcct gtaacttacg agtttcacta cttcaacagc cctgacgagt tcatttgcgt
     9721 catccaacac aacggagttt tctatcaaca gcttgcactc ggtcaaagtc tatccgcgaa
     9781 cattggcggg actggctggt ggatggaggg atcttatgac aataggtatc tgagtacgaa
     9841 taagggctat tcgagcgata gttcaggcgc aaacgcctct gggagctggc aatatcaagg
     9901 tttccagttt ttcgcaatgg gtcaatacgg accgagaggg atgtggctga attatggagt
     9961 ggatgggacc aacgcgtggg gcgcggaggc gaatctaaat tccggctggg tcaactggat
    10021 gatgatgcaa gcgtctccta accaattgaa ccaaggaacc atcttggttc cgattcgcgt
    10081 gatggcttcg cgtccttcaa gtctctatac ggaagtcctc actgtttcca acatccgcat
    10141 gtgccgcaac gacgcatttg atccgggagc gatctacacg ctcggcacgg acaaatggaa
    10201 gatctacccg ttctattcga agaacactgc gcagcgaaac ggagtagcct ggggaacggg
    10261 cgcgttccat tctgggactt acgcctacgc tgtccgctat cagggtccat gatatgaccg
    10321 cgctgctcgg gaagcaggcc gcatctcctc tttacggtgg agcgaacccg gccgtatctt
    10381 cggatatgaa tgggtatacg acgccgtatg cctacacgcc ctatccatgg cttggctctg
    10441 gcgctctgaa agccaacgcg ccggccggaa cgcagcgaat tcctccgcca aacacatggg
    10501 cgatcatcgg taaggcgtcg cctgctttca tgcaggactt ctattatcgt gttctcgttt
    10561 ctccgagcac ccttgcattg ggcacagtgg caagctcgca gacccgcgac gtcgtcgtct
    10621 ggaacgccta tcccgatcag attgcgaatc tgagttccat ctcgatcagc gatcctgtgg
    10681 gtattgcagt gacaggacaa gcggcaccct attcgatgtc tgcactccag gaattgaagt
    10741 atacggttgg aatctcgacc tccggtgcag caaatatcga tgcgtatgtt tcatttaact
    10801 tctcgaatgt ggcttctccg cttcctgttc acatcacagg ctcgcgtgcg atcaagttcg
    10861 acatcatgcc agaagtcccg gttcaagaag attgggagtg gaagagtgat gtcgaggttg
    10921 ctatcgacgg gacagagcag cgtatcgcgt tgcgaggaga gatgcctcgc atcacgcaaa
    10981 ccgtgaaggt tctgttcgac aacaatcccg atgtccggcg catgatctcg cagctgatgt
    11041 ccagcgtcgg acgtctctgg attcctcagt tccagtacgc tgtgaaagtg acgcaggcaa
    11101 cagctacagg cgggaaccgt ctttacttcg accccacaag gacggacatt cgtgttggcg
    11161 agtatctgat gatccagact cccgacggta cgactgcgct gatgggtgtc agtgcattga
    11221 acgcagacgg cgtaaccctg agcggaaccc ttgcgctgga tattccgctg cgctctatca
    11281 tcatgcctgg cgtatcgtcg gtgctccctg atgcgcaagc ggtgactcgc tatgcggtcg
    11341 acagtgtggg caccgttggc ctagtgtgcc gcatgctgtg gtcacgcacc agcttgattc
    11401 ggcctggcgc agcggcggtc gtgttgccta ccttcctcgg ctatccgatt ttagagaagc
    11461 gcccgtcggc agacagcgat atcaaggaac aagcctacac gggtcagcag gatgtcgaca
    11521 acgaaactgg cccgctggac attgtgagtc gatgggttta tactcgctgg ggaggcgata
    11581 ggtcattcct tgtgaatcgc attgccgatc caacggtgat ggattactgg aagacgttcc
    11641 tggcgtatac gcgggggcag tgcaaggtct tctggatgcc gagtttccgt caggacttca
    11701 tcccgatgtc ggtagttgct ccgggggcta acagtttcgc aatcgacgga actgattatg
    11761 cggagaatct gtttccgctc ctgacgcaca agtaccttcg cattaccaca gcagcgggca
    11821 cgcaggacgt gactgtcacg caagcgggat cgcagaatgg acttagcttc ttgacgttca
    11881 gtccggcact tccgacaggt gcaggatgga acaatgtgca attcctttcg ctgatgctgc
    11941 cttgccgctt gacagacgac aaggtgactt ggaaacactc gggtctgtct tcgacgctgg
    12001 acatgactgt aaggactgct gaggtatgac ctatcgcgat caagagaaca gtcagtccgg
    12061 aggcgctccg ttcgagctgt atgagtggat tggcacttat gccagttatt actacacgtc
    12121 ggacctgttc ccgcatatct tcaacggcat cacgtatcag ccgttagcag gccttactcg
    12181 cggaagtatc aaggcaggga cgcatgctga ggacaatacg gatctgaccg taacgctgcc
    12241 gatcacatct cagttggcgc aggactacgc gtttcaggtc actcccccgt cgctccaatt
    12301 gaatatctat cgctttcagc gcgatgcgac tctctcaacg gattacgaag ttgcctggag
    12361 cggcccggtc aattcgatca ctgtcgcgaa cgaaaaggca tcgttcattg ttccatcgat
    12421 gttcgggaac ttgctgcgag gtgcggttcc gaacgtctac atccaacccc cgtgcaataa
    12481 cactctgttc gacgcgcaat gcaaagtgag ccgcgctgct aactcggtcg cgacgaccgt
    12541 aacagccaac gcaggtctaa ctctgacagt tggtccgctc actgccttcc cgcccggttg
    12601 gtttatcggc ggcgaagtcg ttgcgagcgg aacgaacgaa cgacggatga ttatcaacca
    12661 agccgggaac gtcttgacct tgaactattc gttcggcaag ctcgcgatcg gtgcgcaggt
    12721 gcaggtctcg tcaggctgcg atcatagcta tacgggcgcc aatggttgtc cgaagtacaa
    12781 caatcaagtc aatttcggcg gttgtccgtt tgtgccagga gagacgaatg atccttacgc
    12841 gaagggattg cagccatgaa ttgggctagt atcatcctgg gcccgctcac agtcaagatt
    12901 ctggattggc tgttcaagac gcctgcgcaa gcagacgcga ccgcagccaa attcggcgac
    12961 ttcaatatcc ctcgctcgaa gtacggcgat cctgccccat tgctttacgg cactgcgcgt
    13021 cagaagtctc cgctcacgct gtggtttggc gactacaacc ctgtccccat caagaagaag
    13081 caaagcacag gtctgttcag ttcgaaacga gtgattgtcg gatacaagaa ctatatcggc
    13141 atggacatgc ttctgagcat cggcccaggg catgtgctga aacagatttg ggtagacaat
    13201 acctcaatct ggacgggcaa tctcaccacg gacagctcga tcaacatcaa caagccgaac
    13261 cagttcggtg gagacgatca gcgtggcggc tatgtaggca agatgtcgtt ctacacgggt
    13321 ggaagctccc cggtacagaa cgcatatctg aagaagatcc tcggaccgaa cgtgcctgcc
    13381 tacaacggct tctgccgtgt ggtcatggag gcattctata ttggcacgtc cgcgcaggtg
    13441 cccgttccct cgttcgaaat gtcgcgcctc acgaatggcc tgagtgcatc gtattcgatc
    13501 atgccgaacg gactcgattt gaacccgctg gagatcgcat acgacgcatt cacacagcaa
    13561 tatgggcgct tcggtttgcc cgtggcggtg atcgatctgc ccagctggct ggcagcagca
    13621 cagaccctct acaacgaggg catgggcatg acgttcttga tccagtccgc catcaccggc
    13681 aaggacttgc tcaacgagct catggacgtt tccgacggct tcatgtatca ggaaccgtcc
    13741 aaccgcaaga tggttgtcaa gctcgtgcgc gcggattaca acgtcgcgac gctggacacc
    13801 ctcgaccagt ctatcgttag cgatatctct aacttcggca agaatacgtg ggaccagacg
    13861 ttcaaccagt gccgcgtgac gtttgcggat cgtgctaaca gctatgacga ttcgattgcg
    13921 atgcaaacgg actttgcgaa tatcaacttc cagcagaagg tggtttccac aaaccttacc
    13981 agtgccggca tacgcgacgc cactgtggcc aataagtatg ccgcgtcgca gctaagccgc
    14041 gcgaacgtgc cgctatatac ggccacccta aagtgcaacc gcaaggctgc taagtaccgc
    14101 ccaggcgatg tcgttttgct gaactggagt ccgttcggtt tgacgggcat ggtgatgcgt
    14161 atcggtaagg tggagcttgg cgaactcgtc aagggcgtcg ttactctgac ggtggcacag
    14221 gatcgttaca gcaccagcac gccgatcttc gctgctccgc cgcccaccta ctggcccccg
    14281 gttactttcc agcccgcgcc ggtcactgtt cgaaagatta tcgaactacc atacttcttc
    14341 ggctcagttg cgcaaccgcc aggagcgacg gatggtattc tccaggcgct ggcatcgcct
    14401 ccggcctctg cctcgatttc gtatcgagca gacatgactg caaatgcgga tacgaccttc
    14461 gccaatgcga caacggtcat ggaggatgcg ccgtacaacg gatccggaac gctgcgggat
    14521 gcttatgctg cactcacggg tcgttcgaca ggtcaagacg cggtaggttt cgctgtcaac
    14581 ctgtggctgc cgaccagcgc agttccgcct tcgcctggag cattcgacaa taccggacag
    14641 acgttgctcg tcatcgataa cgagatcatc ggttataccg gatgcgtagt gaatccggat
    14701 ggaagctatc agttcagtgg aattcgtcgt gcgcttcttg acacggactt tgctgaccac
    14761 agtgctggcg cgcaggtcta tttcctctcc ggacaagacg atcagttcaa cgcgtacatc
    14821 aacaacacga gcacgcagcg cattcgcttg atcgacaagg ctgcgaccgc tacgctcgac
    14881 ccgtcgctcg cactggtaga cgtgttcacg ctgagccagc gcacgtacaa gcctgcgcct
    14941 ccggactatg tgcagctgaa cggttcacgc acgccgatca gccagtacgt aagtgcagcg
    15001 attgctgtca catggcgtga gcgcaatcgc aagaccgcga cgctcgtgca gtacaacgat
    15061 ccgactgagg tcgctgagcc tggcaccgac tacggtatac gctacagggt gaacgcgggc
    15121 gcgtggtcag cacgcacgtt cgtcactgga gtcagctata ctatcccggc gcttggcaac
    15181 agctacgcgg gaacggtcga cgttgaagtc gttagccgac gtgacggagt catctccaac
    15241 gttggcgaca tctgtacgat cgttctgaca atcgaaacct tccacttgat gacgagtgcg
    15301 aacgaactgc tgaccagctc tggcggcgat tatctgagga ccatgtaatg gctgaccaaa
    15361 ttctaaatct gaacggtaag caggcagcgg cggggagcgg tgttatcaac atcgttcctg
    15421 cggactatat ctccggcctc aagatggtat gggtcagtgc aaccgccctg acagtgacta
    15481 ccggcgcagc gtgccgcccg agcgattctc tgctaatggc cccagcagca aacatcgcca
    15541 agaccgctct tgctctaacg gcctcgacgt ggtatcacgt ctatctctac atgaacggag
    15601 cgaatccgga tatcgagatc gtgacgacag cgccggcagc accgtactct ggcaccgcat
    15661 gcgcgaaaac gggcgacacg tcgcggcggt atatcggaag cgtgctgacg gatgcgagcg
    15721 gcaacatcat ccggttcgat cagaccggga accgcatcga ctacatgatg agcatcaaca
    15781 acgctccctt gcatccgctg aattacgctg gggcgacgat cacgactccg gtcagtctag
    15841 ccgcagcaat tccggtcact gctcgacgcg cggccatgta ccttgaaaac agcacgacga
    15901 atggatctat cgcatatatc tcggtcccgc cgttggcctc acttgccgat gtaattactt
    15961 ttgtccgccc gggagggcgt ctgtctgcac tctatgttgt cgatagcacg caatccttca
    16021 attacgcatt caacggggcg cccgcagcgg gcaccggctt ggacgcatgg gtcaccggtt
    16081 atgtctacga acgctgattg aagcgagact ttcctttccc ctgaccccgc accgcgcgac
    16141 aatcagttgg cccgtgcagc ctacctgagt cgattgcgct cagttcccgt gccgcgatag
    16201 atctggaatc cgcttcctgc ttcgcggcca gctgcgcctg ttgcgttgta gactttcaat
    16261 tccgatgttg tggtcggtcc gactttcagt cctgtgctgc gcaccgcaag ctccgttcgt
    16321 gcagcttcct cgtcggtgaa accgattgcc attacctttg accacacttc gtcgatccaa
    16381 cctatgaaga atccggagcg agcgcccttc ttgcctttcc attccggatt agccttcagg
    16441 tgagcattcc aatcgcggtt catggctctc cagacgactt ggtgagcata ggctgcggtg
    16501 atgacgcggc cagtcggtcc aaaatacttg accgagtagc tgtagtcgga ctgtcgcaac
    16561 gtggacgaca tgatcggacg cacaccgaac gcctgcttca tgagtgcaac gaagtatgag
    16621 atatatgcag gcagcttgac gttcgtctgc accggaatct cgacctcctc gaagccgtag
    16681 cccagcgcgt cgatctcttc ctgagagaca ccatgcttgt ccatgagctt ctgggcttgg
    16741 cgtaacgcag ccgcagcctc gtgcgcattg ctgctcttgc cgagtgcgag gcacttctta
    16801 atcttctcga ggatgcgggc cttatcgtcc attgatatag tccatgttga gcgtcccgtc
    16861 ggcgttgcac tgcgcatgat agacacggtt gaagtccggc caactgtggt catgcaccat
    16921 ctggcaatac tggcgagcgt cctgttctac gctcttgtct tccggaagga acacgtagag
    16981 caagaccaga atgacgaaaa gaaagatggt gatcgcgcgt gacttgtggg ttggcatgtc
    17041 agcttccttt gtcgagttgt tggataaggg aatcgatctg ttctagatct cgtgcgtatc
    17101 cgtctttgac ttcttgctga caagacggac aattggccat ttcacctatg cggtgccgca
    17161 taagggctcg cgcgcgtact agcgcaatga gcagcgcccc gggcgagtca gttgacaggt
    17221 acatggtcga agtctggacc ataaccgaag ttgagatcgt tggcccatac gaccggcgcg
    17281 cgggctgggt aaggtgcgca gccacacagt acgggtattg ctgcgcgacc cgctttgccg
    17341 gctacaggcc gcgcgctacc cttacccttt ggggtagttg cggggtgtac cctgcgcgcg
    17401 gcctgtagca gcatacgcgc ggtgagcagc tcgcgctgag ctgcatgagc gaggccaatg
    17461 tactgcggcg agtacgggcg aacgtgcttc atcaacgctt cggaacgctg cgcgctcttg
    17521 atgttgcgct tggcggaatg gagcatctcg attgcgagct gtgcgtcttt catcggtcgt
    17581 aatcctcgaa gtcgtaatcc cgctcatatt cgctttcgcg gtatgagaac ttcgcgctgc
    17641 gggcatctgc ttcgtacagc gcatctgcaa tcttatcgag tcccttcggg agatcgtcgt
    17701 aagagatctc gtcatcgtcg atgtagtagg tcgggtcgcc tacattcaca tcctccggtt
    17761 cccagaaggt cgcaggcgaa gacgtataac cgtactcgca cttgagtatc gacccattgc
    17821 tcatttcgaa tgtgacggtt acaagagaca tgacgcactc cgctctggtc tatgggtgta
    17881 atatacaccc gttagcgcag aatgcaacga ctttttagac gtatccaatt tgcttcagga
    17941 ttttttcaga ttccttgaca taccagtcga aatcaacatc ctgagggaac tcttccgtaa
    18001 gcgtcatcat cggacgtgcg cccatcgagc gaggcacccg cttgccagac ttgccgtaaa
    18061 tgagcggacc ctctacttcg ttgctgtagt accagcgaat gtgactgccg aagtattccc
    18121 catctttgac cgctccgcca cttaccttgc gcacggtcag gaatttagtg atgtcgcgac
    18181 acttcgtgat tgtggtcaca atgggaacgc ccttagtgag catcgcttcg atagcatcga
    18241 tgcagatcac attgacaggg ttcttgtgca tccacattga tttgtcagaa gcatcattcc
    18301 aagggttggc gtaagcgccc ttcgtcttgc atttgtcagg ttgctcgatc cagactttgt
    18361 tctctttatc aaacttctgc ttgacggcga tgtagttatt cacatcgcgg gaatagagag
    18421 acatataacg agtctcttcc aactcgaagc atgtctcgcg ctcccattct tttacgatcg
    18481 aatacatgac cgaatgcatc gtgcgtttgc acttcacgac gataccatcc gtgttggcgc
    18541 tgaccacatg gatgcccgca agctcgagct tctcgatcag atatagcagc gacagctgac
    18601 ccgtcatggt cacctgcacc agcaggtcgg gcgcatacag attcgaatac ttgcttccga
    18661 gcttgccgaa cgaaccgttc acaaggatct tgaagccgtt cgccttcacc atgtccttaa
    18721 tgcgcttagc gacgatgcgc tcttcgacct ttgcattgaa cgcgcgcagg aagttcggac
    18781 cgagatggtg cgggaacaat ccctgattga tcatgatacg cggatagaac gaagtcacgt
    18841 caaagtcgtg cagcgtgtat tcgctatccg tgtgatgcgc aatcttcttc tctttcgagt
    18901 gcagtccgcc gatacccatt gcgtacttac tgtcggcaat ctggagatcc cacagcttct
    18961 cgaactgctt tggttcctcg accgccccat ggaatgcgac aagaaacgga gcgatacgca
    19021 cttcgttcag aacccagcgt agcaactccg ttctgaaatt aagatagctc gggatcttgt
    19081 aaaaatacat cgttcccgga gcgacgatag gtttgaatgc acgtcgtccg ctcatctgct
    19141 cgacttcgag cgagaacaca tcctctgcta tctgcgcatc agaacgcgaa cgcagatcaa
    19201 gaccgtacat gcgtcccacg tcaatgcgaa gttgaatctg ctcttgaagg gtgagccaga
    19261 gaagctcagt gttgtcgaga tcgttcacgc agtagtagcg tgtcacacag atctgatcgt
    19321 cgctgagcca cttgtccggc gcaaagggta gatcctgcat gcgtcggcag aacaagcgtc
    19381 ccgagtacgc cttcagaccc ccatgcagcg gcgcgacttc tttcaagtcg atatggttta
    19441 gcttgagctt cttgactcgc cgctctttca gaatctgata cggcgagatc tcctgctcga
    19501 taagctcgtt ggatgcctgc ttgatctcct gcgtcgtctt gcccgccagc gcaagcgcaa
    19561 gcatggtcaa gtcgtacgac agaccgttaa aggaaatcgt ctgaaagttt tccaggatcc
    19621 acaacagctt agagcggtcc aactctgtca gagcaccgtc gacggtttca atgtatgtca
    19681 cccgcttcgt atcggggcac ttgaacgcgc agaggaaata gttcgaatag acttcgcaat
    19741 caagcgtgag attgcagccc attgcttcca caagttctgc atcgctgaac tgcgggaaat
    19801 tgaatgcgcg cgcttcgctc agatagggga ggtaatcgtc gttgagccat accgggtcag
    19861 gtggcgtaac tttgaccttt tctttcacga ccttgacgac aggctcatct ctccagaaca
    19921 tgccgattgc atcgcgcctc acagacgcaa tccgataatg gcaccgcgca tgcggtcacc
    19981 cttgaagaac aggcctgggg cagggaacga ctgtgcatcg aaaaactcga caccttccag
    20041 aagagacagc atctccaccc tatagctgct cagaggccat gccgcgttct cgtgaagcgt
    20101 gtaggacgat ccttcttcga gcgagtcgtg cgtgcgcacc gtgcaaccct ccaggattac
    20161 tcggccgttc ttgtcacaga acggcttcac tgcttcgagc gccttgaaaa gttcttcgct
    20221 gagtggcgtt gcaccgtggt cacggttcag aatcgagtcc acgtcgggcc attcggttga
    20281 gaagagttgc gtgcggagcc acctgccgtc ctcgtattcg aatgtgacag aattgctctc
    20341 tacgcgcatg cgaacaggcg cttggttgat gcgcaggatc tcagcgaccg cagagcgggg
    20401 caggttgacc acgctgggca gcgcgaaccc catatagaac tgggccaggc agacgttgtt
    20461 cgtcgcgtaa gcgctcccgt tccggatcag gataccgttc gtgaaggggc gtgatgcatc
    20521 attgcctacg aacggttgga gctcctggaa cgcgcgcagc agcagcgcgc cgtcgaagct
    20581 cacttcgtcg ccgctaggct cagcgtgcgg ggagtcgtct tccgtgcatt caatgaatgc
    20641 cttgaacttg ccgctgcgca cggacaagcg tccggcagcg gtcaagctaa gctgaatcgt
    20701 gtcctcgcag cgagcgatcg cttgtaccat cggtccggct ttcggtttgc agtcgaggtc
    20761 gcagctgatt ggactgctca gcgcaagggt tccgttgaat gcagtgacac gaccgtcctt
    20821 gatctgaaag tgcgtcatcg caggaatgaa atcctttttg gcgactgccc cctgtacgaa
    20881 cttcagttct ttgaacatta cggcgttatc tccaaagtga ccacgcaatt cttgaaatcg
    20941 taactcacat ctattttatt ctttgaaagg tcgcagctat cttgaatttt ggatatgcgc
    21001 ttacgcaatt cgccataatc tcccggctgt tcggaatagt attggaccac aatgcgctta
    21061 cacaaaatgt ccgaaggaag catctcagct acagcagaga gcaaaagttc tttcttcatc
    21121 agaatagctc ctgaaccatg tcacggaaac gcggagaatg gtgagcgttg atcatcgtgt
    21181 tgatcacgcc atacgcccat aggttgtagg ctgcgcgcga ttcatacacc gtcgacagac
    21241 gctcatatgt gaacccttgc tgttcgagca tcttgaacac gtaatcgcat tcgatctgcg
    21301 tgagtgtggt tacgtgctgt cccgcgtcgt gcctcgacgg actcttttcc gagacattaa
    21361 gcggtccgtg atggggggaa acaatagatc caaaagcggc actctgaatc caggacgagg
    21421 agtcacagga ataccacgga taacgctcca tgagaggaat ggaggtaatt ccgaacccat
    21481 gaacgcgcag cttgggcttt ccagctccgt cgaccagatg cttgtcccag attcggtcca
    21541 accaacgcat gagctgttcg gttgacgcac ccaccatgcc gccgagcgtg atataagggt
    21601 agtttgcgac ataatagtcg aggtatcgac tgtcttcgcc tgaatggaaa cagggaagag
    21661 gtcggacacc acgcgcctcc atctcgagtt ggttgcggta ggtttgcagg gggtcgccaa
    21721 taccgtccag aaccgacgcc attacgacgc catcttcctt gcggataatg tccgaattgc
    21781 gttggatata attgcagtat tcctcgacgg acagggtcac gcccaaggtg tacgcggaga
    21841 acgcgccact atccagaaag atctgtgcgc tatccgcacg catttcatct acgaacttct
    21901 gcttgcccac atagtgccag gactcgagga tatgggggat ttcttcgacc aatcgacgct
    21961 cgtggtcgtt gagcttgaca tagcggttct gccccggcat gtagctattg gagtaaaccg
    22021 ctgccataaa gatgttcatg cgtcttttgt ccgtgagttg aacgcgggtt tcagggattc
    22081 gcctccgctt agctttggct gcgtcatcct ctagaagatg cccttacacg tatacgcccg
    22141 cgcagaccca ttatacaacg cctgttagtt aactcgcaag cgcaaagaac tcagctcgag
    22201 tcgcgacctc gtcacgaatg acgccgcgca atgcagacgt aatcgtatgg tggccctgct
    22261 ggcagacacc acgagactcc atgcacatgt gccgagcctt gatgaccaca cctacgccga
    22321 gaggttgcaa gtgttcgacc agcgcgtcag cgacttgctt cgtcagacgc tcctgaacct
    22381 gcaagcgacg tgcatacgca tccagcacac gggacagctt ggacaagccg acgaccttgc
    22441 cattcgacgg aatgtaggcg atggttgcgg tgccgaagat gtcggccatg tggtgctcgc
    22501 acttgctgta gaacggcacg ttcttgacga taacgaattg gtcgtaaccc tcgccaccat
    22561 cttcgaactg cgtcttgagc acttcggcaa tatcgatgtt gtaaccgccg aaccagtgat
    22621 cgaacgcctt agatacgcgc ttcggcgtat cttccagtcc ttgccgcaac ccgtaacctt
    22681 cgggaacggt ctcgactcgc aagagaagct gacgaatcag gtcttccttg gtgagttcgt
    22741 gaggaaagag attgcgggtg atgactcggt cagaggctga catagcttgc tccatttgca
    22801 ccgtgctcgt acacggtcgt tttgtaaaga tcgacgcggc cctgggtctg gatcttcgtc
    22861 cagcgggcga cccattcgtg gatatactgc gcaagggcct cacacccgac gttcggaagc
    22921 acgatcacgt tggcgatacc gatgccggcg agcatgcaga tgtcgtcttt gaatgggtca
    22981 tcttccgaca cgagcatggt gtggtcaagc agattctcga gccatttttt gacttccttg
    23041 aacccaccga aatccatcac ccagttcttg tcgtccagac aatcgctcat gaactcgaac
    23101 ttgacgccca gggcgtagcc gtggagaagg gagcagtgcg aatgcttcgc cttccattga
    23161 cggaaacagc aagaccagcc ttgctcgttt ccgaagttct tgtaggagag atgtttaccg
    23221 ctcatattcg atgacttcct tctggttgtt gatctcgaat gcggtcttgc gcatgaagca
    23281 cggtccgcag tcgccgcaat gcttgtcgtg cgcgtgatag cagctccacg tcagatcgag
    23341 cggcgcaccg atctcgagac ccattgccac aatctcgtgt ttcatgagat tgccgacagg
    23401 catctcaacg cgcatgcgct tgttgacgcc gacagcgaac ggaagcagat cggagaagcg
    23461 tcgcacgaac tccggttcgt tgtccgggta ggcaccagcc tcctcgagat tgttgcccag
    23521 aacgatggtg tcgaacccat ttgcttccgc gtaggcagtc gcaaccgaca tcagaatcag
    23581 gttgcgtgct gggacccact cgtgagcgaa ctctgcaccc gactcaccct tggccacccc
    23641 gttgctttcg cgcagcaggg gcgaatctgc gggcttgtat acgggcagcg ggaaccgggt
    23701 aagggtaacc ccaaggcgag ccgcgatcgc cgttacagcc gcgacctcgg ggccctgcgc
    23761 acgcgaccca tattcgaaat gaataagctc tacttcgtaa cctcggcgga tcatctcgac
    23821 cgctgcaacc gtactgtcaa gtccgccgct ggcaatgaca agtgccttga agctcgcacc
    23881 ctgactgcga tgcgccatct tgcggcagcc gagctgcccg ttctgaccga cgtagaaaat
    23941 cgcgtgcgtg tagggttcga ccatctccgg aacgaaaccc tgcgggaaat agtctcgcga
    24001 gctcgcgaag aagaaacccc agggcgtcgt gatgtaccag acgggccggt aattgcagga
    24061 tacgagaaca gcgcggtcat atcctgccgc agtcgccagc accgcatagc tcccgaccag
    24121 catttccatc gcattaccga agccggccag cacttgatct attgaatagc gatcgtcggg
    24181 cttatatccc tccagcaact ccgcaattgc agcgctgtcg atttgcgtgc gcaggcgatg
    24241 cgtgcgcagt tccgcatcgt tggcgatggt gccgttatgt acgattgacc acgcgcctgc
    24301 gatgtagggc tgctgatcgc tactggcctt ctccttgacc cattcggttg tcggttctgc
    24361 gcgcatgttg ccaatgatgt tgccgaacat gaatgtgcgt cggaaatact caccatcgta
    24421 aggaggaacc atcggcttgc cgaaatggtc ggtcgtggtg tgctgcggca ccgcgcggtc
    24481 accccacatc ttgccatcag catgacactg gtaaccccag ccgtctcgac ctcgctcttc
    24541 cgaggaaagc atgatcgctg cgaggatctt gttgacggtt tcgaggtcgt gtttggtttg
    24601 cacgttgcgc agcaacgcgc cgaaaattgc gcacatgatt atgccactcc gataagtttg
    24661 tggatctgga gttggaggat gtaaccgtgc ttcaggcagg actcgacgca agccttgaga
    24721 ttgaacgcat tgcgcgtttc gtcctgttcg tcagtcggtt gcaggtagat gtctttgtcg
    24781 cccttggggc gaggcacgcc ttttgcggaa gggtgctcca gcgggcgcaa gggaaggcca
    24841 tcttctttgg acaggttgtg ccgaccgccg acgtacttgt atgcgcagca gtgctgatga
    24901 atcttcggat gcactttgcc agcctttggg ctgcacacaa tatagacgcc gtttcgctcc
    24961 caggcattgt gattgtagaa ccagtgatcc agatcggtgt tctccggttc ctcgggaggt
    25021 gcgagcgtgc cgttggattc gacctggaca aagaactcgg tgcagaacag aaggtcgaac
    25081 aagtttgaca gctcctggcg gaacggttct ccaccagtaa tgaccacaag cctcgtcttg
    25141 ttcaaacctt ccgtcagatt cagcacttca cgaaggatct cgtgcgtctt catcatcttg
    25201 cggccggtgg tgtagttcgt atcgcactgc gggcattgca agttgcatcc cgcaagtcgc
    25261 acgaaaacgg ccggcgttcc gcagaacggg ccttcacctt ggatcgtatg gaaaatggaa
    25321 tggacggaaa gttcacgtcc atcggactca acccgcttct cgggtttctg agtgttcagc
    25381 tgcatgtcgg ctccgggata agtggcacag ggcgcattgc gcgccctgtg cggggtgacc
    25441 cactggcaag cgtagcagct tactgcgccg ggtgctcgga ggtctgcggg gcctgggcc
//
LOCUS       DATMTI010000655         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_618773, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000655 DATMTI010000000
VERSION     DATMTI010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618773"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2091)
                     /locus_tag="VN039_10580"
     CDS             complement(<1..2091)
                     /locus_tag="VN039_10580"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWV46444.1"
                     /translation="MSRLRLALVIVLAIVVLAVAFLSFSRELTGQDYLKDFVLEQLEE
                     SLGRKIDVHRVKFVIFPRIRVELTDVKIHDRESEQVVLTAKRVDLVLRLLPLLKKQVV
                     GKRLLIEEPTLTLRRNERGRWNVLEGLNGQADSDQPTMDMMARTFMISEATLVNGTIT
                     VIDAARPDGIRSLELEHVEFRLLVRPERSLAELHLSAAHQGESGVSAVSLDGAIKRAG
                     PSLSLSGEETTESTTGFQFEGQIDAADLKIRDVADFLGPRPVSKHLQGALNLKSTVRV
                     MPGVAGYDMVLSDMTAHLNNIMLKGKANLAGLLTPQPTFTVTFSSSLVALSQLLKTIP
                     PDWIHPQLPALLVDRQIDGKVQVVNATLTGVATTGPRLSTIGEFHVSEGRGLIGSDRI
                     AAKDLAAAVVVETGRVRIAKITGMYGAMQITGGKAEVSFINAGPWLELDITGEMAATH
                     LIEFLARTVKAERVTQLFAGIRDADGTVQSTFRLEGLLNRSDGITFAGGEITAHEVSL
                     THVNLPERLTGMQGRFVLADGSTQFDQVSGHLGETTVQVQGAITGGAASQFQNFVVRT
                     RGSAAQIVRLFRSTGGEQGMFEGMLSAEIALSGLTTRPQVRGSAVLDEAKVVVGAVEK
                     PVGAHATVEFEGEMTKGTNLTLHRVELILPTVHIPAKGTMKFGDRFMIDMAVATGTLT
                     VSGLPEWMSKGGLEA"
BASE COUNT          466 a          643 c          537 g          474 t
ORIGIN      
        1 cgcctcaagc ccacctttgg acatccattc cggcagaccg gataccgtca atgtacccgt
       61 tgccacggcc atgtcgatca tgaatcgatc accgaatttc attgtaccct tcgctggaat
      121 atgtactgta ggtaagatca gttctacccg atgtagcgtg aggttggtgc ctttggtcat
      181 ctcgccttca aactccaccg tcgcgtgggc accgacgggc ttttccaccg ccccaacgac
      241 tacttttgct tcatcgagca cggcggatcc gcggacctgg ggtctcgtcg tcaaacccga
      301 cagggcaatc tccgcgctga gcataccttc gaacataccc tgctcgcccc cggtagatcg
      361 gaacagcctc acgatctgtg ccgcactacc gcgcgtgcga accacaaagt tttggaactg
      421 gcttgccgcc cccccggtga tcgccccttg gacttggacg gttgtttcac cgaggtgtcc
      481 cgagacttga tcaaactgcg tggatccatc ggccaaaaca aaccttccct gcatcccggt
      541 caaacgctcc ggtaaattca catgggtaag actgacttca tgggccgtaa tctccccacc
      601 ggcaaacgtg atcccatcgg aacggttcaa cagtccctcg aggcgaaacg tcgactgcac
      661 cgtaccgtcg gcatcacgga taccggcgaa caactgcgtg actcgctccg ctttcacggt
      721 cctcgccaga aactctatga gatgcgttgc cgccatctca cccgtgatgt ccagttccag
      781 ccatggacct gcgttgataa acgacacctc tgcttttcca ccggtgatct gcatcgctcc
      841 gtacattccg gttatcttcg caatgcgcac acgtcctgtc tcgaccacga ccgctgcagc
      901 gaggtccttc gcagctatac gatcactgcc gatcaagcct cgaccttcgc tgacatgaaa
      961 ctctccgatc gtcgagagtc ggggaccggt cgtcgcaacc ccggtgagcg tcgcattcac
     1021 cacttgcacc ttgccgtcga tctggcgatc caccagcaga gctggaagct gcggatggat
     1081 ccagtcaggg gggatggttt ttagcagttg agagagggca acgagagaac tgctgaatgt
     1141 gacggtaaac gtcggctgcg gagtcaggag accggcgaga ttggcctttc ctttcagcat
     1201 gatattgttt aaatgcgcag tcatgtcgga caggaccatg tcatatccgg caacgcccgg
     1261 catgacacgg acggtgctct tcagatttaa cgctccttga agatgcttag aaaccggcct
     1321 gggaccgaga aagtcagcta cgtcccgtat tttcagatca gcggcgtcga tctgtccttc
     1381 aaactgaaat cctgtggtcg attcggttgt ttcttcaccg gacaacgaca acgatgggcc
     1441 tgctcgtttg atggcaccat ccagcgacac ggcagatacg cccgattctc cttgatgagc
     1501 cgccgacagg tgcaattctg cgaggctacg ttcaggcctg accagtagtc tgaactcgac
     1561 gtgttctaac tcgagagatc gaataccgtc tggcctggcg gcatcaatga ccgtgatcgt
     1621 tccgttaacg agtgtcgcct cgctgatcat gaatgtccgc gccatcatgt ccatggttgg
     1681 ctggtcggaa tccgcctgac cattcaaccc ctccaggaca ttccaacgac cgcgttcgtt
     1741 ccgccgaagt gtcagtgtcg gttcctcaat cagcagtcgt ttgcccacaa cttgcttttt
     1801 gagaagcggc aagaggcgca agaccagatc gacccgctta gccgtcaaga caacctgctc
     1861 tgattcacga tcgtgaatct tgacatcggt gagttccacg cgaatacggg ggaagatcac
     1921 aaacttgacg cgatgcacgt cgatctttct acccaggctc tcctcaagct gttcgaggac
     1981 aaagtctttg agatagtctt ggccggtcag ttctcttgaa aatgaaagga acgcgacggc
     2041 caataccacg atggcgagta cgatcacgag cgcaagacga agacgagaca cattatcccc
     2101 ttcaaattac ataccggtcc
//
LOCUS       DATMTI010000656         6230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_322511, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000656 DATMTI010000000
VERSION     DATMTI010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322511"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VN039_10585"
     CDS             <1..1726
                     /locus_tag="VN039_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HWV46445.1"
                     /translation="LSPGASEIIRLRLTNRTEPVGFKVQAFNELFAQRIYEANEFYDE
                     LAPADLSEDAKLVQRQAFAGLLWSKQFYQYDLKRWLVGDPGMPDPPHERLHGRNSDWT
                     HLYNADVISMPDKWEYPWYAAWDLAFHCIPLALVDSTFAKEQLILMLREWYMHPNGQI
                     PAYEWAFGDVNPPVHAWAAWRVYKIERKRSGVGDRVFLERVFHKLLLNFTWWVNRKDT
                     EGKNIFQGGFLGLDNIGVFDRSAPLPTGGHIEQSDATSSMGMYCLNMLSIALELAREN
                     RAYEDVASKFFEHFVYICRAMNNIGGEKIELWDREDGFFYDVLHLPDGRTFPLKVRSL
                     VGLIPLFAVETLDSELIDSLPRFKHRMQWFIENRSDLSAHIETHSYDGGVRRFLSLVN
                     PTRLKSVLRYMLDEEEFLSPYGIRALSRYHKDHPYVLSILGRNYRVDYEPAESSTGLF
                     GGNSNWRGPIWFPVNYLLIESLQKFHYYLGDEYRVEYPVRSGQFVSLHEVASELSRRL
                     THIFLRDKNGRRPVYGGAGKFQEDFFWRDTILFYEYFHGDNGAGIGASHQTGWTGLVA
                     KLIQQSGE"
     gene            1874..3871
                     /locus_tag="VN039_10590"
     CDS             1874..3871
                     /locus_tag="VN039_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWV46446.1"
                     /translation="MKIGKKECQNLDRALSLEWLETNGRGGFASGTVSGANTRRYHAL
                     LLAAQYPGNHRYALVNHVEEWLRIDGEEVSLSTNLYPGTVHPRGYTHCTSFASTPWPT
                     WTFVCKGKKFQREIVCVRGRNIVIVRWTLLEMANDSPALRARPMLTGRDYHALHHENE
                     KLSAQTTINEGMVTWESYQGLPVVRAFHSGTYRHASDWYRRVQFPLEQERGLDFEEDW
                     WSPGEFLFKLEPGKEEALAFCSETIDRLDIADLDHKERVRRARVQRSAPDVDRLANVL
                     WQAVDSFLVQRSTQHTMIAGYPWFTDWGRDTFISLPGLCLVTGRHEIAWKIIQTFSSY
                     VSEGMVPNRFPDLSEDPEYNSIDASLWFVHAIDRYLTYSEDATRIRSIAWPAIKQILD
                     GYRQGTRYGIHMDADGLIAGGTSGIQLTWMDVKIGDWVVTPRHGKPVEIQALWLRALD
                     VGARLAALFGELNYAIRCQDDRARGVESFREKFWYQDGQYLYDTINGPEGDDASIRPN
                     QIFAIALCDDLLTREQATQVLHIAKDHLLTPVGLRTLSPTDVRYRPRYEGGPMERDSA
                     YHQGTVWPFLLGPFVTSWVKTFGRTSETRAESRSFLNGIEAHVQEACLGQVAEIFDGD
                     PPHHPRGCPAQAWSVAEPLRALVEDLGVPITTGAVRPSRHK"
     gene            3996..4547
                     /locus_tag="VN039_10595"
     CDS             3996..4547
                     /locus_tag="VN039_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWV46447.1"
                     /translation="MALFIPNQNGRMVVAYMVMLSLLGIAAPGVTFADELSKESVLPL
                     GTANKAIQTALEACKKDGYRVSVSVVDRAGVLRAMGRADGAGPHTVDSSRKKAYTAAS
                     VRRPTSELADLITKVPTLQALRDMNAETLILGGGLPIEIGGEIVGGIGVGGAPGAHLD
                     DACAQEGLDAIGAAPKVPTSKSK"
     gene            4673..4996
                     /locus_tag="VN039_10600"
     CDS             4673..4996
                     /locus_tag="VN039_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46448.1"
                     /translation="MATDPFLQRFNLTLNVKGTNGCSSSTELFPDTGYAGRRNVYQAK
                     GSVYVVGQYDARVIDPQSCRTSLKEFRHLDRDVIFMGSFDQDEEKRWRYFSAAQRLEL
                     PFEKR"
     gene            5436..6059
                     /locus_tag="VN039_10605"
     CDS             5436..6059
                     /locus_tag="VN039_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWV46449.1"
                     /translation="MATVEKPALSDVDHRDGFFIHRIQKGYAVWIGVLLFLYSALFFT
                     LAFYGAHLKPMLTLYGSDALEERQVAAAEMLMLSETVWVAVPVLFFGAAIFSLIITRR
                     IAGPLYRLDEGLQQWVKGNLRWRMRFRPSDRLDELAATVNQALENIEQSFARIQQQTH
                     MLQAALSKIEQDRSLGIEEARDAMEDIAEALRRFDFRSLRKTHVKTP"
BASE COUNT         1402 a         1559 c         1806 g         1463 t
ORIGIN      
        1 actgtctccg ggcgcatcag agatcattcg ccttcgtctg accaatagaa cagagccggt
       61 ggggtttaaa gttcaagcgt tcaatgaact ttttgcgcag cgtatctacg aagccaatga
      121 gttttacgac gaactggctc cggctgatct ttccgaggac gcaaagttgg tacaacgaca
      181 ggcgttcgct ggattactgt ggagtaaaca gttctaccag tatgacttga aacgctggct
      241 tgtcggtgat ccagggatgc cggatcctcc acatgaacgt ctccatggac ggaactctga
      301 ttggacgcat ctttacaatg ccgacgtgat ctcgatgccg gacaaatggg agtatccctg
      361 gtatgccgct tgggacttgg cattccattg cattccgctc gcccttgttg attcgacctt
      421 cgccaaggaa cagctgatct taatgttgag ggaatggtac atgcacccga acggtcaaat
      481 tccggcgtat gagtgggcgt ttggcgatgt caatccgccg gttcatgcct gggctgcatg
      541 gcgagtctat aagatcgaga gaaagcggag cggggtcggt gatcgggtct ttctcgagcg
      601 agtgttccat aagttgctct tgaactttac atggtgggtc aatcgcaaag atactgaggg
      661 caagaacatc tttcaaggcg gatttttagg actcgacaac atcggcgtct ttgatcggag
      721 cgctccgttg ccgaccggtg gtcatatcga acagtcggat gcgacaagtt cgatggggat
      781 gtattgtctc aatatgcttt cgatcgcgct agagttggcc cgcgagaatc gggcatacga
      841 ggatgtggcg agcaagttct ttgagcactt cgtctacatc tgccgagcca tgaacaacat
      901 cggcggggaa aaaatcgaac tctgggatag agaagacgga tttttctatg atgtgctgca
      961 tcttcctgat ggacgaacct ttccgctcaa ggttcgatct ctcgtcggac tgatcccgct
     1021 ttttgccgta gaaacgttgg actcggagtt gatcgatagc cttccacggt tcaagcatcg
     1081 catgcagtgg ttcatcgaaa atcgatccga tctcagtgct cacattgaga cgcattcata
     1141 tgacggagga gtacggcggt ttttgtcctt ggtcaatccc acacgactca agtcggtgtt
     1201 gcggtatatg ctcgacgaag aagaatttct ctccccgtac ggcatccgag cgctctcacg
     1261 ctaccacaag gaccatcctt atgtgctttc tatcctcggc aggaactatc gggtggacta
     1321 tgaacctgcc gaatcgagca caggactgtt cggagggaat tcaaattggc gaggccccat
     1381 ctggtttccg gtcaactatc tgctgatcga atcgcttcag aagttccact actatctcgg
     1441 cgatgagtac agggttgagt atccggtgcg ctccggtcaa ttcgtctctt tacatgaggt
     1501 tgcttccgaa ctgtcgcgcc ggctgactca tattttcctc cgagacaaaa atggccgacg
     1561 tccggtctat ggtggcgcag gcaaattcca ggaagatttc ttctggcgag acacgattct
     1621 tttctacgag tattttcatg gtgataacgg cgcgggtata ggagccagtc atcagacagg
     1681 atggaccggc cttgtcgcca agttgattca gcagtcgggg gagtagggac gtgagggcgg
     1741 cacggactga gcagtttctt cgctcgcgca acgcgcacgc aaggtctgtt caagaccgga
     1801 atatcggggg gtgctcgttg catgcgcgcg gtgagaaatc gctcaatccg tgccgcccga
     1861 gatgtggacg aaggtgaaga ttggaaagaa agagtgtcag aatctcgatc gcgccctgag
     1921 tctcgaatgg ttggagacga acggtcgcgg agggtttgct tcgggaacgg tttccggtgc
     1981 caatacgcgc cggtaccatg ccctgttgtt ggcggcgcaa tatccgggaa accatcgata
     2041 tgccctcgtc aatcacgtcg aagaatggct tcgcatagac ggcgaggagg tttctctctc
     2101 cacgaatctg tatccgggaa cagtccaccc acgcgggtac acgcactgta cttcattcgc
     2161 atcaaccccc tggccgacct ggacatttgt ttgtaagggt aagaagtttc aacgtgaaat
     2221 tgtgtgtgtt cgcggtcgca acatcgtcat cgtgcggtgg acgttgctcg aaatggcgaa
     2281 cgactctcca gcgctccgag cgcggccgat gctgaccggc cgcgactacc acgccctgca
     2341 ccacgagaac gaaaagcttt ccgcgcaaac cacgatcaat gaagggatgg tgacctggga
     2401 gtcatatcaa ggtcttccgg tcgtgagggc cttccactcc ggaacctatc gtcatgcctc
     2461 cgattggtat cgacgggtgc agttccctct agaacaggaa cgtggacttg atttcgaaga
     2521 agattggtgg tcaccgggag aattcttatt caagttagag ccaggcaagg aggaggctct
     2581 ggcattttgc agtgaaacga ttgacaggct tgatatcgcg gatctcgatc acaaagaacg
     2641 tgtccggcgt gcgcgggtac aaaggtccgc gccggacgtc gaccgattgg ccaacgtatt
     2701 gtggcaggcg gtggattcat tcttggtcca gcgaagcact cagcacacga tgatcgcggg
     2761 gtatccatgg tttacggatt gggggcgaga cacctttatc tcgcttcccg ggctctgtct
     2821 cgttacagga cggcatgaaa tcgcatggaa gattattcag actttttctt cctatgtttc
     2881 agaaggcatg gtgcccaatc gctttccgga tctgagcgaa gacccggaat ataactccat
     2941 tgatgcatca ctgtggttcg tccatgccat cgaccggtac ctgacataca gtgaagatgc
     3001 aacacgtatc cggtcgatcg cttggccggc gattaagcag attctcgacg gctatcgcca
     3061 aggcacgcga tacggcatcc atatggatgc cgatggtctc atcgctggcg gcacatccgg
     3121 tatccaactt acctggatgg atgtgaaaat cggtgattgg gtcgtcacgc cacggcatgg
     3181 aaagccggta gagattcagg ctttgtggct tagggccctg gatgtcggtg cgcgacttgc
     3241 tgctctattt ggcgaactga attatgccat acgctgtcag gatgataggg cacgaggagt
     3301 ggaatccttc cgcgaaaaat tttggtatca agacggacag tatttgtacg atacgatcaa
     3361 tgggccggaa ggcgacgacg catccattcg ccccaatcaa atttttgcga tcgccctgtg
     3421 tgatgatctg ctgacgaggg aacaggcaac acaggttctc cacatcgcca aggaccatct
     3481 ccttacgccg gtgggattga gaacgttgtc gccgacggac gttcgatatc gtcctcgata
     3541 cgaaggcggc cctatggaac gagatagtgc ctatcatcag ggaaccgtct ggccgttcct
     3601 gttaggcccc tttgtgacgt catgggtgaa aacatttggg cgtacgagtg aaacacgagc
     3661 ggagagtcgg tcctttctca atggaataga agcgcatgta caggaagcct gtctcgggca
     3721 ggtagcggaa atttttgatg gggatcctcc ccatcatcca cgaggatgtc cggcgcaagc
     3781 atggtccgtt gcggagcctc tccgtgcact ggttgaagac ctcggggtac cgattacgac
     3841 cggagcagtc cgcccatctc gccacaaata agccattgcc acattgaagc aatgaaacaa
     3901 taattgaatc agagccgtcg gcttctgcgc tcttgacaga gttcggatgc atccgtaggg
     3961 tgaatgtgcc ggatacgttg ggaggacaaa gtacgatggc actctttatc ccgaaccaaa
     4021 atggccggat ggtcgttgca tacatggtga tgttgtccct attaggcata gcggcacccg
     4081 gcgtgacctt tgccgacgag ttatcgaagg agtccgtact gccgcttggc acggccaaca
     4141 aggcaattca aacggcattg gaagcttgca agaaggacgg gtatcgagta agcgtttccg
     4201 tggtagatcg tgcaggcgtc ctgcgcgcga tgggacgtgc cgatggggcc ggaccgcata
     4261 cggttgatag cagtcggaag aaagcctata cggccgccag cgtgcggcgt cctaccagtg
     4321 aattagccga cctcatcacg aaggtcccca cgcttcaggc tctgcgtgac atgaatgccg
     4381 agaccttgat actgggtgga gggcttccca tcgaaatcgg cggagagatt gttgggggaa
     4441 tcggtgtggg tggagcaccg ggcgctcacc tggatgatgc ctgcgcccag gaggggctcg
     4501 acgcgatcgg tgcagctcca aaggttccta cgtcgaagtc gaagtgaaag tccggcgatg
     4561 gtctgccatc tgtggaagta tggcgcttct ctgcataggg tgcaacagta atactcccga
     4621 gccggcttcg gttgaaataa cctcctcagg acttcggctg acgatcgtga ggatggcgac
     4681 ggatccgttc ctccagcgat ttaatctcac gctgaacgtc aaggggacga acggctgttc
     4741 gtcttcaact gaattgtttc cggatacggg ctatgccggt cgacgaaatg tttatcaggc
     4801 aaagggaagc gtgtatgtgg tcgggcagta tgatgcgcgc gttatcgatc cgcagagctg
     4861 ccgaacaagc ctcaaagagt ttcgccatct ggatcgcgac gtgatattta tgggaagctt
     4921 cgatcaagat gaggaaaagc gatggaggta tttttctgcc gctcagcgtc tcgaactgcc
     4981 tttcgagaaa cgttgacgtc gggctgagaa gaatcattga agaaacacat cagtgacttg
     5041 atcaatgggc atggatttct acgccattcc tgctacatcg tattcatcac gacatgcaac
     5101 agccgatcgt cggatatcac caggatgaat atggcgattg ggttgccgat ctggcctgtg
     5161 gtcatggaca acacgtacgg catcagcctc cattcacgaa tcgcccatgg gtgctcaccg
     5221 tcgccggacg cggccagtat gtggggacca atttgaattg caagaagtgc gacgaaccgc
     5281 ctgccgagac tcctccgact tcgagataaa ttctgcacca tctctttttg ccgtcatgaa
     5341 cgatccattt acttggccgg tggcggcctc gatgataccg tgagtcgcag agatatgggg
     5401 gtgtcatgaa atgaccccat gaaggaaggg tgcatatggc caccgtcgag aaacccgcac
     5461 tatccgacgt cgatcacaga gatgggtttt tcatccatcg gatccagaag ggttatgctg
     5521 tctggatcgg agtgctgctg ttcctttact cggccctgtt cttcacgctg gctttctatg
     5581 gcgcccatct caagcccatg ctgacactgt atggcagtga tgcattggag gagcgtcagg
     5641 ttgccgcggc ggaaatgctg atgctgagcg aaacggtctg ggtcgctgtt ccggtcctgt
     5701 tctttggcgc ggcgatcttc agcctcatca tcacacggcg gatcgccggc ccattgtatc
     5761 ggctcgatga gggtcttcag cagtgggtca agggcaatct tcgctggaga atgcggtttc
     5821 gtccgagcga ccgattggac gagctggctg cgaccgtcaa ccaggctttg gaaaatatcg
     5881 aacagtcctt tgcccgcatt cagcagcaga cccacatgct tcaagccgcg ctgtcgaaga
     5941 tcgagcagga tcggtcttta ggaattgaag aagcgcggga tgccatggaa gatattgctg
     6001 aagcattgcg acggtttgac ttcagatcct tgagaaaaac gcatgtgaag actccatgac
     6061 tcagcgactc ggcaaggatt ccggattcac gttgatcgag ttcatgatag tcatggccat
     6121 actcggtatt ttggctgctg tggccatggt atcgcacagg cattttgcgg aaaaggcgag
     6181 gtttgtggag gcggaggtgg cattggccga gatcaaccgc ctagaaactc
//
LOCUS       DATMTI010000657         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_152290, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000657 DATMTI010000000
VERSION     DATMTI010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152290"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1054..1245)
                     /locus_tag="VN039_10610"
     CDS             complement(1054..1245)
                     /locus_tag="VN039_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46450.1"
                     /translation="MQSLPFVRQEEDEGGAMTIGYNTHAETMIRLLNEALAAEIICIL
                     RYKRHYFRTAGVRPRHVKM"
     gene            1566..>2207
                     /locus_tag="VN039_10615"
     CDS             1566..>2207
                     /locus_tag="VN039_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46451.1"
                     /translation="MYETIGRALQLPQGQLAQLAEQQRREQLRKRLGDQPTPLLHDVR
                     ELILRKCHPKKTRQIRGIFEKQHFGELERFVTQKLLGVVKSVARQELDNPTWVRKVAR
                     LSKKSYPQMRVTVLEFLDTTIFDLSNENCIAFLDPLIESWDIDLATFCIEIVLNRRVA
                     PGHHKTFEFLERGPDKTSEEEPGLKEFLQDPLLSGDATEEELTFLRTLTFENKR"
BASE COUNT          501 a          637 c          587 g          482 t
ORIGIN      
        1 aggctgcagc atcctcggaa atctattcgc gtctggtcaa aataggacag cttgcaagca
       61 ttcacgaaag catatggctg cctacagcgc ctgccatgcc ccgtgatcca tggggtgata
      121 ggatctgccc ctggcgaatg ccggcatcgc ccgtcagaaa gacccgcaat ccctatggga
      181 cttctggagg aactagtcgt ggaggagaac atttaaggag ggcgctgatc gaggtgaggc
      241 cctgatggag cttgtcaccg gtacgaacat aaccgccacc agaggcttgc ccagcaatga
      301 gctctcactc cctattacgg cttttcagtt gccggtcgtt ccacccaacc gactaaagtc
      361 aggaaaatca cagtcaaaac gaggaacccc ggcgccgcct tcatcgcgct gttccattcg
      421 agccgttcgc gttgctcggc cagattcaac tcgctcgccg ctgtggccgc tcatctatga
      481 tgaaccgcgc gatgcgttgg cattgtcggt cagcgactca aatagtcgca actgctccgc
      541 aaggctgtct ttcacgcgcc gacgcggcga gcgtccactg cctttcagag gactcggctc
      601 agaggccgac ccctgtccac gcctcatctg tgatacgtgg ccacgccgcc ttatggtttc
      661 acccctcatt taagtgcctc cttacgatca gaaagagcgc agtgcatatg tacacagcga
      721 ggctcttcca gcatcctggg tcaattctct gttgagtatt cacggtccca gcgacgctca
      781 tcggatcgat gcatgctcct gagagtcact cttactaaac acgacctctc ctttcttgac
      841 tttcctgcgc ctgtgaggaa tcgccccgat gatctccgaa cggcggaagc gattcgcacg
      901 aatgacggtg tcctcgatac acccacaatt gatgcagcgt gtgccggtgt ataaagagtc
      961 cgtttcgatg ttcaggtcat cgaaggtttc acataccagt agccctcgac agcgttggca
     1021 gaccatatgt cctcctttct gcgcctagtg tgttcacatc ttcacgtggc gaggcctgac
     1081 cccggcggtc ctgaagtaat ggcgcttgta gcggaggatg cagataattt ctgcggccag
     1141 cgcttcgttc agcaacctga tcatagtctc cgcgtgggtg ttgtatccga tggtcatagc
     1201 gccgccttca tcctcttctt gtcttacaaa tggcaacgac tgcattatgt tcacccatga
     1261 cggctcatgt atgagcgcac ccggcatctc cccatcgaat tgggaccagg cctcttggct
     1321 gtgtcagaag cattcccaac ctcttcgtta tggctcagca taatgaagta gacggctgcc
     1381 gtctgttcac aattgctcag tagggaatga tgacaagatg gtgacgaggc cgaactcggc
     1441 ctggctctac agaatcaaag aagtggaagc agttggagac gctagtcatg aagcattgag
     1501 gagagaacta tgcacatgca gttgctcaca caaaaaaact cccgccggcg ccgaaccgga
     1561 ccgacatgta cgagacgatc gggcgagcat tgcaattgcc gcagggacag cttgcccaac
     1621 tcgccgagca gcagcgccgc gaacagctcc ggaaacggct cggagaccag ccgactcctt
     1681 tgctgcatga cgtgcgggaa ttgattctcc gcaagtgtca tcccaaaaag acgaggcaga
     1741 tccgcgggat ttttgagaag caacacttcg gcgaactcga gcggttcgtt acgcagaaac
     1801 tgctcggcgt cgtcaaaagt gtcgctcggc aggaactcga caatcccacg tgggtgcgca
     1861 aggtggcccg actgagcaag aagagctatc cgcagatgcg cgtcaccgtg ctggagtttt
     1921 tggacacgac gatcttcgat ctctcgaacg agaactgcat tgcgttcctc gatcccttga
     1981 tcgaatcctg ggatattgac cttgccacct tttgcattga gattgttctg aaccgtcggg
     2041 tcgcgccagg gcaccacaag acgttcgagt tcctggaacg aggaccggac aagacttcag
     2101 aggaagagcc tgggctgaag gaatttctcc aagacccctt actgagcggg gatgcgaccg
     2161 aggaggaact cacctttcta cgcacactga cgtttgaaaa caaacgc
//
LOCUS       DATMTI010000658         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_661661, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000658 DATMTI010000000
VERSION     DATMTI010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661661"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VN039_10620"
     CDS             complement(<1..257)
                     /locus_tag="VN039_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46452.1"
                     /translation="MPVSSSYSRSILAVTIAVFLLAFSFNAYACLLPLSGTDDVSMAN
                     GCSTPVEQPVRQLCDAFKTLGVESSPQASSLHMDHHWSAAD"
     gene            complement(330..3068)
                     /locus_tag="VN039_10625"
     CDS             complement(330..3068)
                     /locus_tag="VN039_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWV46453.1"
                     /translation="MADRSIGGCCGSQHQGANDMSKQIPMTQISSHTGTDIDPVCGMR
                     VDPATAAGHYNYQGKTYYFCAVSCLDTFKADPERFLTSAPADLISLGKKKPLPMMMPA
                     QPAPAIGEIDPVCGMAVQPTTAAASYEYRGKKYYFCAARCLEKFRADPEYYLTPPEQR
                     TPKPTPVSASGITKYVCPMDPEVLETKPGACPICGMALEPADVTAASTRTEYTCPMHS
                     EIVQAEPGNCPICGMALEPRTVAAEEVNPELVDMTRRFWQSVVLGSPILALMISEMMP
                     GQPLQHLFSGKVLIWFQFVLATPVVLWTGLPLFQRAWASVVNRHLNMFTLIGLGTGAA
                     YFYSVAATLVPGMFPDSFRVHGGELAVYFEPAVAIIALVLLGQVLELRARSRTSSALK
                     ALLGLAPKTARIIRGDAREEDIPLEQVQVGDRLRVRPGEKIPVDGVVLEGSSVVDESM
                     VTGEPIPVEKQSGQKVVGATVNGTGSFVMRAERIGRETLLAQIVRMVSEAQRTRAPIQ
                     RLADVVAAYFVPIVILVAAVTFVIWALYGPEPRMAYALLNAVAVLIIACPCALGLATP
                     MSIMVGTGRGATAGVLIRNAEALEAMAKVDVLVVDKTGTLTEGKPRLMTVAPLPHVTE
                     TDLLKLAAGLEQSSEHPLAAAIVSGTRDKGILPSKVENFRSLTGKGVTGMIEGRAVAV
                     GTALLLNELGIETKPLVAQAEPLRQAGQTVMFAAIDGKPAGLLGVADPVKSSTPEAIE
                     LLHREGLRLVMLTGDNRTTAESVARRLQIDEVQAEVLPEQKAAVIKRLQAEGHVVAMA
                     GDGINDAPALAQAQVGIAMGTGTDVAMESAGVTLVKGDLRAIARARRLSRGTMRNIRQ
                     NLFFAFVYNMLGVPVAAGVLYPFVGVLLSPMIASAAMTFSSVSVIANALRLRKMAL"
BASE COUNT          716 a         1031 c          884 g          644 t
ORIGIN      
        1 tggtctgccg ctgaccaatg atggtccatg tgcaacgaag acgcctgagg ggaagactca
       61 acccctagag ttttgaaggc atcgcacaac tgtcgaacgg gctgctccac gggggttgaa
      121 cagccgtttg ccatcgagac atcatcggtt cccgacagtg ggaggagaca ggcgtatgca
      181 ttgaaactga acgccagcag gaacactgcg atggtcacgg cgagaatcga ccgggagtaa
      241 gacgaactta ctggcatgga gcaagtctat ccttccatca acttaaccgt caacggtaat
      301 tctcatccgt cagtactcgc atgaaccatt tacagagcca tcttccgcaa tcggagtgcg
      361 ttcgcgatga ccgacacgga gctgaacgtc attgcggcac ttgcgatcat ggggctcaag
      421 aggaccccga cgaaaggata gaggaccccg gctgcgacag gcacgcccag catgttgtag
      481 acaaaggcga agaacagatt ctgccggatg ttccgcatcg tcccacggct caagcgtcga
      541 gcccgggcga tggctcgaag atcgccctta accagcgtga cgccggcgct ttccatcgcc
      601 acatccgtcc ccgtccccat ggcaatcccc acctgcgctt gcgccaaagc cggtgcatcg
      661 ttgatgccgt ctcccgccat ggccacgacg tgcccttcag cctggagccg cttgatgacc
      721 gccgcctttt gctcaggcaa aacttcagcc tggacttcat cgatctggag ccgacgggcc
      781 acagactcgg ctgtcgtccg gttgtcgccg gtcagcatca cgagccgtag tccttcacga
      841 tgcaacaatt ctatggcctc gggtgtggag gacttgaccg gatcagccac gcccaacaac
      901 cctgccggct tgccgtcgat cgccgcgaac atgacggttt ggcccgcctg tctcagaggc
      961 tcggcctggg ccaccagcgg tttcgtctcg atacccaact cgttaagaag cagcgcagta
     1021 ccgacggcta ctgcacgacc ttcgatcatg ccggtgacgc ccttccctgt gagagaacga
     1081 aagttctcta ccttcgatgg aagaattccc ttgtcccggg tgccggatac gatggccgcc
     1141 gccaacggat gctcactgga ttgctccagg ccggctgcga gctttaacag gtcggtctcg
     1201 gtgacatgcg gcagcggcgc caccgtcatc aggcgtggct tgccttctgt caacgtccct
     1261 gtcttatcaa ccaccagaac atcgaccttg gccatggcct ccagtgcctc cgcattacgg
     1321 atcaacactc ccgccgtcgc tcctcggccg gtcccgacca tgatcgacat cggcgtcgcg
     1381 agccctaatg cacaggggca agcgatgatc aatacggcaa cggcattgag caaggcatag
     1441 gccatacgcg gttccgggcc gtacagtgcc cagatcacaa aagtaactgc ggcgaccaag
     1501 atcacgatcg gcacgaaata ggctgccacg acatcagcca gccgttgaat cggagccctg
     1561 gtccgctgtg cctcactgac catgcggacg atctgcgcca gcagcgtctc acgaccgatc
     1621 cgctcggccc gcatcacaaa actacccgtc ccattgaccg ttgctcccac cactttttgg
     1681 cccgactgtt tctccaccgg gatcggctcg ccggtcacca ttgattcatc gacaacactt
     1741 gagccctcca gcactactcc atcgaccgga atcttttcgc ccggtctaac acgcaaccga
     1801 tcgccgacct gcacctgttc caacgggata tcttcttcgc gagcatcacc gcgaatgata
     1861 cgggccgttt tcggcgcgag tcctaacagg gccttgaggg cactgctggt tcggctacgg
     1921 gcccgcagct ccaacacctg tcctaataag acaagagcga tgatggcgac agccggctca
     1981 aagtagacgg caagctcgcc cccgtggaca cggaacgagt cgggaaacat tccaggaacg
     2041 agcgtagcgg cgacactata gaaataggct gcgccggtgc cgaggccgat cagagtaaac
     2101 atattgagat ggcgattgac gaccgaagcc caggcccgtt gaaagagcgg caagccggtc
     2161 cagagcacca caggagtcgc cagcacaaac tggaaccaaa tcaatacttt tcctgaaaag
     2221 aggtgttgta aaggctgtcc cggcatcatc tccgaaatca tcagcgccag gataggtgaa
     2281 ccgaggacca cgctctgcca gaaccgcctg gtcatatcca ccagttcagg attcacttcc
     2341 tccgccgcca ccgtgcgagg ctctaacgcc atcccgcaaa tcggacaatt tccaggctcg
     2401 gcttgcacga tctcagaatg catggggcag gtatattccg tgcgcgtact cgcagccgtt
     2461 acatcagccg gctccaatgc cataccgcat atgggacatg ctcccggttt tgtctcgagc
     2521 acctcggggt ccatcgggca aacatacttc gtgattccgc tcgccgatac gggcgtcggt
     2581 ttcggagtcc gttgttccgg tggggtgaga taatattcgg gatcggctcg gaacttctcg
     2641 agacacctgg ccgcacagaa atagtatttc ttaccgcgat actcgtacga agcggccgcc
     2701 gtggtaggct gtaccgccat cccgcacact gggtcgatct cgccgattgc cggcgccggc
     2761 tgagccggca tcatcatggg caacggcttt ttcttcccca atgaaatgag atcggccggc
     2821 gccgacgtca agaatcgttc cggatcggct ttgaatgtat cgagacagga taccgcgcag
     2881 aaatagtacg tcttgccttg gtagttgtaa tgccctgccg cagtggcggg gtccaccctc
     2941 atcccgcata ccggatcgat gtccgtccca gtgtgcgaag aaatttgagt catcggtatt
     3001 tgcttgctca tgtcgttcgc tccctgatgc tgactcccgc aacagccgcc tatcgatcga
     3061 tcggccatgg ttcgtctcct ttcatcttat ccggtctgct tgctcaatgt cgccttcacc
     3121 cctaaagacg gaccacggcg cccaatatta cagagagccg gccggcacga ttgaaaacga
     3181 tcagaaaaga ggcagagaca ggagaggacg gctagccgca ggtgcagttc aagcagccat
     3241 gtctactgca gattccgcaa gcgtcctcca gagaa
//
LOCUS       DATMTI010000659         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_238510, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000659 DATMTI010000000
VERSION     DATMTI010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238510"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..497)
                     /locus_tag="VN039_10630"
     CDS             complement(261..497)
                     /locus_tag="VN039_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWV46454.1"
                     /translation="MKSELLQRITIEPGKCGGRPCIRGMRIRVQDILDMLAAGTSEEE
                     ILRDYPYLERDDIRAAITYTAAYLNHTIVPAQTR"
     gene            complement(1176..1595)
                     /locus_tag="VN039_10635"
     CDS             complement(1176..1595)
                     /locus_tag="VN039_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HWV46455.1"
                     /translation="MSVGEQKDSVQTTPYQEAGELAGITRLVDEFYINMDTLPEAETI
                     RSMHPPDLTESRMKLTYFLCGWLGGPRLFQQHYGPISIPAFHKQFPIGYEERDAWLLC
                     MQRAIAVQPYTDQFKDYLLAALNIPAERVREVNAGEF"
     gene            complement(1617..2708)
                     /locus_tag="VN039_10640"
     CDS             complement(1617..2708)
                     /locus_tag="VN039_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HWV46456.1"
                     /translation="MKRSRLHGQHAQLGATFEDVTGWEIPAHYGDVAAEHRAVRQTVG
                     IADLSHRGKLRVTGEDRVKWLQSVISNDILPLQPGQGRYSSFLTHKGKMLTYFRLYMQ
                     TEAVMLEDVGEIGDTTFQALRKFLLYGTKAKMENCAESWGLLLISGPKAPHVVQSAFG
                     VNVTDLKPVDFVTAQIGGHHALVLRTEETGEVDIEVLLPTDSLLPAWASAMQAGDKFG
                     MRVIGTHAREALRIEAGLPKAGSDLNEEIVPPEANLEEKAFSLNKGCYPGQEVVARMD
                     TYGNVRRKLVGLVLKDSIVPPHGAKLYSGDREVGWISSAVRSPQLNKAIAFGFPLRDF
                     SKPGTELAVEFESGRHPATVQTLPFYAKH"
BASE COUNT          573 a          723 c          775 g          664 t
ORIGIN      
        1 agccaatttc gcagcttcgc gtgcgtcgca ttgccgaagc gtatccacac gaccgcagcc
       61 ggtttgccac cgagcgtcgc gagggtggca aagtcttcat ccttcgtgac aataatgaat
      121 ctgttccggc gggcaaactc aaagatagcg cgatcatcga gcgctaaacc gggctgatcg
      181 ttgacgtgaa ttgcttcgtg gttgtgctcg cgaagccaaa gcagagaggc ctggcgggag
      241 gttcgcgtcc acgagaaatt tcatcgggtc tgtgccggca cgatcgtatg gttcaggtat
      301 gcagcggtat aggtgatcgc ggctcggata tcatcacgct ccaagtaagg ataatcacgc
      361 agaatctctt cttcagacgt acctgccgcc agcatatcca gaatgtcttg gacacggatc
      421 cgcatgccgc ggatgcaagg gcgtcccccg catttgcctg gctcaatcgt gatacgttgg
      481 agaagctcag acttcataga ggtaggtcta cccttctcgt acactgggtt tctctgaggg
      541 caccatagca ttggccttga ggttgagcaa tcaggctggg gaactcgccc ctgtgctgca
      601 tgtcagcgta atcaaggacg actacagtgg gtggttcata aagtcacgga gggactggat
      661 agttttcacg ttgcagttag tccaggcctt cggcggagcc tcgccgaagc ttccgacata
      721 ggagagcgaa tgatttcgtc agtgaaccgt ggacaagagt tgaacgtgag tggtcttcag
      781 aggctctgat gtccccagcg atcgggtgca ctgaattgtt tcttgcagaa aaccggctca
      841 cgtcgatcca ggcttcgcat ccgatcgaga ttggttccac ctggagagag caggagatgt
      901 ggtgctcgtt cgagttgaag aagaaagcca tctcgtcttt catgaatttc ggcccaccgt
      961 gaccctttca ttgtagatag agcaaggaga atgccacccg aatggaggaa cagcttggtc
     1021 gatttttcgt tcatcgcgtt gattttgaag ggtagcggtt tctcagacgg acctgatgaa
     1081 gcctaagtct ctgtatccgg aaggatgcag cgatgtattc caacatacgg gagtaatcaa
     1141 atggaactgt tcagccaagg caagaggcgt gggagctaga actctcctgc gttcacttct
     1201 cgcactcgtt cggctgggat attgagtgcg gctaagaggt agtctttgaa ttggtcggtg
     1261 tagggctgaa cggcgatggc tcgttgcatg cagagcaacc aggcatcgcg ctcttcgtac
     1321 ccgatgggaa actgcttgtg gaatgctgga atgctgatcg gcccatagtg ctgctgaaac
     1381 aacctcgggc ctcccagcca gccgcataga aaataggtca gcttcatgcg tgattcggtt
     1441 aaatccgggg gatgcatgct tctgatggtt tcagcttcag gcagtgtatc catattgatg
     1501 tagaactcgt ccaccaagcg agtgattccc gcaagttcac ccgcttcttg atacggagtg
     1561 gtttgcacgc tgtctttctg ttcaccaacg gacatatgtc ttcgtggggt ctggtgttaa
     1621 tgtttggcgt agaagggcag ggtttgaacg gtggccggat gtctgccgct ctcgaattcc
     1681 acggccagtt ccgtgccggg tttactgaag tcgcgcagag gaaatccgaa cgcaatagcc
     1741 ttgttgagtt gaggggaacg gacggcgctg ctgatccagc ccacctcacg atcaccgctg
     1801 tacagttttg ctccatgcgg cggaacgatt gaatccttta gcaccagccc gaccagcttg
     1861 cggcgcacgt tgccgtaggt atccatgcgt gccacgacct cttgccccgg atagcaccct
     1921 ttgttcaggc tgaaagcctt ttcctcaaga ttagcttcgg gcgggacaat ctcttcgttc
     1981 aaatccgacc cggccttcgg gaggcctgct tcgatgcgca aggcttctcg tgcgtgggtt
     2041 ccgattactc tcatgccgaa cttgtctccg gcctgcatgg cactagccca agcgggtagg
     2101 aggctatccg tggggagcag cacctcaata tcaacttctc ctgtttcttc ggtgcgcaac
     2161 accagggcgt gatgtccgcc gatctgtgct gtgacaaaat cgaccggctt taagtccgtg
     2221 acgttcacac cgaacgcgga ttgcactaca tgcggggcct tcggtccgct gatcaacagc
     2281 agtccccaac tctcagcaca gttttccatc ttggctttgg tcccgtagag caggaatttg
     2341 cgtaaagcct ggaacgtggt gtccccgatc tctcccacat cttccagcat gacggcttct
     2401 gtctgcatgt acaggcggaa gtacgtgagc atcttgcctt tgtgagtcaa gaagctggaa
     2461 taacggcctt gtcccggttg gaggggaaga atgtcgttgc tgatgacgct ttgcagccac
     2521 ttcacgcgat cctcgcccgt gactcggagc ttcccgcgat gagagagatc ggctatcccc
     2581 actgtctggc ggacggcacg atgttcggcc gcaacatcgc catagtgggc tggtatttcc
     2641 cagccggtga cgtcctcaaa cgtcgctcca agttgggcat gttgcccatg aagtctagat
     2701 cgtttcatcg ggaatctttc acaccttacc cctta
//
LOCUS       DATMTI010000660         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_746393, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000660 DATMTI010000000
VERSION     DATMTI010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746393"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..991
                     /locus_tag="VN039_10645"
     CDS             281..991
                     /locus_tag="VN039_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46457.1"
                     /translation="MSMLVRAVKVLAWMWLFAACSVILVASASPWYQGGFAALWELVG
                     PHLVINCVFVGLTLAPALFLTTLARGIHEKHRGKILRSAAALLVSVAAVTLIVLAPVK
                     LAKNETQQARQTGKAREYEAQSIRVKDQSATLYQYNAHFIKPSSGPVGTVGIPESIQI
                     GDAITVNGHTLRAQHILVKEILSDINYDGHPLGKAGETQCVIVESLKNLPDADQSDTR
                     DRLWIVVEKCVPIAAATQ"
     gene            complement(1072..2061)
                     /locus_tag="VN039_10650"
     CDS             complement(1072..2061)
                     /locus_tag="VN039_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWV46458.1"
                     /translation="MVLNHTSSISGNPLRLQDRPIPVPKADQVLVKIHVCGVCRTDLH
                     VVEGELPDIPFPLVPGHQAVGTVMQIGSRVSEIKEGDRVGIAWLQGTCGQCGFCTNGR
                     ENLCLQARFTGYQVDGGYAEYAVVPARFAYPIPSIFSDEEAAPLLCAGIIGYRALRLS
                     GIKPGQRLGLYGFGASAHIAIQIARHWGCQVYVSSLKREHQGLARQLGAVWVGGATEM
                     PPDKLHSSIIFAPAGELVPPALRALDRGGTLALAGIHMSPIPALDYDRDLFGERAIRS
                     VTANTRQDGLDLLREAAAIPIKPHTVRFPLEEANRALQELKAGSFQGAAVLTM"
BASE COUNT          467 a          613 c          597 g          488 t
ORIGIN      
        1 aggggccccc aagtcatgac cgacgccggc tcttataccg tgagcgcgcg ctcgcagccg
       61 attaaagact cccggctgca tttaagtgcc atgcgcgcac ccgtggcctt acactcttcg
      121 acgatcaagt catcatacaa cctgagaccg tcagcgatac catcacacaa ggtcccaccc
      181 cggttttctc ctcccctccc gcctttgaca agcatgccat acttcagtgt aatgagagct
      241 gatcgaagag aactggacgt ttcttgatcg gacggaacac atgtcgatgc ttgtccgtgc
      301 tgtaaaggtg ctcgcgtgga tgtggctttt tgcggcctgt tctgtgatcc tggttgcttc
      361 tgcctccccg tggtaccaag gaggcttcgc tgccctttgg gagctggtcg gcccacatct
      421 tgttataaac tgtgttttcg taggtctgac actcgctcca gccttgtttc tcacgacgtt
      481 agcgaggggc attcatgaga agcatcgagg caagatactg cgttcagccg ccgcgctgtt
      541 ggtcagtgtg gccgctgtga cactgatcgt cctggccccc gtcaaactcg cgaagaacga
      601 aacgcaacag gctcggcaga ccggcaaggc cagagaatac gaggcgcagt cgatccgcgt
      661 gaaagaccag agcgcgacgc tgtatcagta caacgcccat ttcataaaac cctcgtcagg
      721 cccagtggga actgtgggga taccggaatc gattcagatc ggcgacgcga tcacagtcaa
      781 tggccacacg cttcgtgccc agcacattct cgtaaaagaa attctgtccg acatcaatta
      841 tgacggtcac ccacttggca aggcagggga gactcagtgt gtcattgtag agtcgctcaa
      901 gaatctaccg gatgctgatc aaagcgacac gcgcgaccgt ctatggattg tggtggagaa
      961 gtgcgttcca atcgccgctg caacccaata ggctggcttg caccccaatg tctccgagac
     1021 aagcgcgttc ttctcgctca catgctgttc gatgagctag gttcagcacg ctcacatcgt
     1081 gaggacggcc gctccttgaa agctccccgc ttttaattct tgtaatgcac ggttcgcttc
     1141 ttcgagaggg aagcggaccg tatgcggttt gatgggaatc gcggctgctt cacgcagcag
     1201 gtcgagtccg tcctgcctgg tattggcggt cacgctgcgg atggcacgtt ccccgaacag
     1261 gtcgcgatcg tagtcgagcg cgggaatagg tgacatgtgg atgccggcca gagccagggt
     1321 gccgcctcgg tcgagcgctc tcagtgccgg agggacgagc tcgccggctg gtgcaaagat
     1381 gattgagcta tgcagcttgt ccggaggcat ctccgttgcc ccgccgaccc agactgctcc
     1441 cagttgtctt gccaatccct gatgctctcg tttgagcgaa ctgacataaa cctgacaacc
     1501 ccaatggcgc gcgatctgga tcgcaatgtg tgcggaggca ccaaatccat acaacccgag
     1561 acgctgaccc ggtttgatgc cgctgagacg caacgctcga tagccgataa tgccggcaca
     1621 taacagagga gcggcttctt cgtcagaaaa gatggatggg attggatagg caaatcgtgc
     1681 ggggacgact gcatattccg cataccctcc gtcgacctga tagccggtaa acctagcttg
     1741 taaacagaga ttttcccttc cgtttgtgca gaatccgcat tgcccacatg ttccctgaag
     1801 ccaagctatc ccgactctgt ccccttcctt gatctctgag accctagagc cgatttgcat
     1861 cactgtgccg actgcctgat gtcctgggac cagggggaag ggaatatccg ggagttctcc
     1921 ttctaccaca tggagatccg tgcgacagac gccacagaca tgaatcttga cgaggacctg
     1981 gtcggccttt gggaccggta tcggtcgatc ttggagccgc aaaggattgc cggatatact
     2041 gcttgtgtga ttgagcacca tcgctttcat cgtatgtctg ttgtctgaaa gtcggaacgt
     2101 atgaggtgag agggtttgaa agttgaacag ccagaacgtt caaggtcttc acgtttcaac
     2161 cttca
//
LOCUS       DATMTI010000661         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_279215, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000661 DATMTI010000000
VERSION     DATMTI010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279215"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VN039_10655"
     CDS             <1..870
                     /locus_tag="VN039_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV46459.1"
                     /translation="AHKGLTISVKIDADAPEEFRGDPIRLRQILFNLVGNAIKFTQHG
                     GVTIAVSVKSKPSDSSEEVVLQWSIQDTGIGLTREQQAQLFTAYVQADASTARRFGGT
                     GLGLMICRQLVELMGGTITVNSVFGQGSTFAYTTNLLPAIHRDPATSSLGTDPCGAGE
                     PVGPLRVLVADDNEINQVVACKFLQKLGCQVEVARTGCEAVEAISRTAYDVVFMDCEM
                     PEMDGYEATREVRRREEGTPNHLPIVALTGHTSDDEVQKCHLAGMDRVVTKPVTLTVL
                     RANLQELLRQVDS"
     gene            948..1520
                     /locus_tag="VN039_10660"
     CDS             948..1520
                     /locus_tag="VN039_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWV46460.1"
                     /translation="MTRTPDGDKFVLYGDFNCPFCYALHERLHDLGLIDRCEWRGVQH
                     APQLPRPMKPWSGSLGAELRHEVGMVQRLAPGLPIALPPGKPNTLPAIEQAIGLLQKD
                     LRNGMDFVQRTYRAFWCDGRDISDPLILKELAGEHFAEDVDGQNQIFAQKWETSWHAT
                     GQNGVPLLVSPEGDLLVGCMPEEQVRQFFL"
     gene            complement(1526..2437)
                     /locus_tag="VN039_10665"
     CDS             complement(1526..2437)
                     /locus_tag="VN039_10665"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV46461.1"
                     /translation="MTDTEARIGFVGVGRMGANMARRLKERGYRITAIQDRHTEVSES
                     LAQELGSEAAVSPARVAEISDIVITVVSDDTAMRNIYAEQDPTSLMAHAKDRLFINCA
                     TLTPALHIDIEQAVERHGGSALEACMASSITQARQGTLYLMCGGRQEVFNRARPMLDT
                     LGTTVRYIGPAGEAAKVKALVNMVMNSNTAALAEGLGLGAALGIDLTLLREVFSQTGA
                     ASRVLETDGEDMQLRAHDCYFSATHAEKDSAIALDLAQNVGLSLPVALATLGQYRRLM
                     ELGKGDLDKSGIAELTFPDRTPSRLLT"
     gene            complement(2477..>3274)
                     /locus_tag="VN039_10670"
     CDS             complement(2477..>3274)
                     /locus_tag="VN039_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46462.1"
                     /translation="ASTSTSGSPPRTGRGVRTETIVSPAPVTLTQTLPAPSNIQDGPQ
                     KAASFDPTSLVGTWHAHVTQGTAQHDMLLVVQPNLKWSLSSMMQFDGILNAEAGLWSL
                     ERANTFKGLGWKGTYRKTTPNAFASTGSVQATWKRLSTDESPTRIPAELWKLRREATS
                     VPVFQLKTVDPDLVGQWEGSGTYAGGSAAFVWAIKPTAATDLLIVDTLRGTVQTKDGI
                     PQLQPIQKKQQRVSVIAFHERGFTTTDGKTNLRWSKFRPESTEDGQL"
BASE COUNT          662 a          874 c          973 g          765 t
ORIGIN      
        1 gcccacaaag gtcttactat ttcggtgaag attgacgccg atgctccgga agagtttcga
       61 ggtgatccga tcaggctgcg acaaatccta ttcaatctcg tcgggaatgc gatcaagttt
      121 actcaacatg gaggggtcac gattgctgtg tctgtaaagt caaaaccgtc ggacagttca
      181 gaggaggtgg tactgcagtg gagcattcaa gatacgggca tcgggctaac ccgtgaacaa
      241 caagctcagt tgttcacggc ctacgtacag gccgacgctt cgaccgcgag acgatttggg
      301 ggaacagggc tcggtctcat gatctgccgt cagctcgtcg aattgatggg agggaccatc
      361 actgtcaaca gtgtgttcgg tcagggaagt acgttcgcct ataccaccaa ccttcttccg
      421 gccattcatc gagatccggc aacatcttcg ctaggcacgg atccatgcgg tgcaggagaa
      481 ccggtggggc cgctacgcgt cctggtcgca gatgacaatg aaatcaatca agttgtcgcc
      541 tgcaagtttt tgcagaagct cggctgccag gtcgaagttg ctcgcacggg ctgtgaagcg
      601 gtggaggcca tcagccgaac agcgtatgac gtcgtgttca tggattgtga gatgcccgag
      661 atggatggct atgaagccac ccgagaagtt cgtcgccgcg aagaagggac gccgaaccat
      721 ctgcccattg tggccctcac cggtcatacg tccgacgatg aagtccagaa gtgtcatctg
      781 gccggcatgg atcgggttgt gacgaagccg gtgacgttga ccgtcctccg tgcgaatctt
      841 caagaattgt tacgccaagt cgactcctga tacagtctgg tgctcctgtc cttcttccta
      901 tcgcagatgg ggtagacacc gtcgagccgg ttcggtagag tgacggcatg acgaggactc
      961 ctgacggcga taaatttgtt ctctacggcg atttcaactg tcccttctgt tacgcgctgc
     1021 acgaacggtt gcacgacttg ggccttatcg accggtgtga gtggcgtgga gtgcagcatg
     1081 caccgcagtt gcctcgcccc atgaagccgt ggagcggctc gttaggagcg gaattgcggc
     1141 acgaagtcgg gatggtgcag cgattggcgc cgggattgcc catcgcgttg ccgcctggga
     1201 agccgaatac tttaccggcg attgaacaag caatcgggct cttacaaaag gatttacgga
     1261 acgggatgga ctttgttcag cggacgtatc gtgcattctg gtgcgacggt cgagacattt
     1321 ccgatccatt gattttaaag gagttggcgg gagaacattt tgctgaagac gttgacgggc
     1381 agaaccagat cttcgcacag aaatgggaaa cttcttggca tgcgacaggt caaaatggag
     1441 ttcctttgct tgtatctccc gagggcgatc tgctcgtcgg atgtatgcct gaagaacagg
     1501 tcagacagtt ctttctgtaa ccgatttacg tcaggagccg agatggagtt ctgtcaggga
     1561 acgtcaattc tgcaatccct gatttatcca aatcgccttt tcccaattcc atcaagcgcc
     1621 ggtattggcc caatgttgcg agcgcgacgg gaagcgagag accgacattc tgtgccaagt
     1681 cgagcgcgat ggcagagtct ttctcggcgt gggttgccga aaagtagcag tcgtgcgcac
     1741 gtagctgcat atcctctccg tccgtttcca atactcgcga ggccgctccg gtctgactga
     1801 atacctcacg cagaagagtc agatcgattc ccagcgccgc ccccagtcct aacccttcgg
     1861 ccaacgcggc ggtattgctg ttcatcacca tgttgacgag ggctttgact tttgccgcct
     1921 cgccggcagg gcctatgtat cggaccgttg tcccgagggt gtccagcatc ggacgggccc
     1981 tgttgaatac ttcctgcctg cctccgcaca tgagatacag tgtgccctgg cgcgcttggg
     2041 ttatgctgct tgccatgcag gcttcaaggg ccgaaccgcc gtgccgttca accgcctgtt
     2101 cgatgtcgat atggagcgcc ggcgtgaggg tcgcacagtt gatgaatagg cgatctttgg
     2161 cgtgagccat caagctggtc gggtcttgtt ccgcgtaaat attccgcatt gccgtatcgt
     2221 ccgatacgac ggtgatgacg atatccgata tttcagcaac gcgtgccggt gatacggctg
     2281 cttccgagcc cagttcctgt gccaaggatt ccgacacttc ggtgtggcgg tcctgaattg
     2341 ccgtgatgcg gtatccccgt tctttcaatc gcctggccat gttggcgccc atccgaccta
     2401 ccccgacgaa cccgatgcga gcttctgtgt ctgtcatgag agagcatcct ttctggagcc
     2461 atacgaccaa tgcgggctaa agctgaccgt cctcagtcga ttcaggccga aatttgctcc
     2521 atcgaaggtt ggtcttccca tcggtcgtgg taaatccacg ttcatggaac gcgatgacgc
     2581 tgacgcgttg ttgcttcttt tgaataggct ggagctgggg aatgccgtct ttggtttgca
     2641 cggttccgcg aagcgtatcg acaatcagca agtcggtcgc agcggtgggt ttgatcgccc
     2701 acacgaaggc ggcggaacct ccggcatagg tgccggaccc ctcccattgg ccgactaagt
     2761 caggatcgac cgtcttaagt tgaaagaccg gtacgctggt tgcttcgcgt cggagtttcc
     2821 acagctctgc tggaatgcgc gttgggcttt catcggtcga gagtctcttc caggttgcct
     2881 gaacactgcc ggtgctggcg aacgcattcg gcgtcgtctt gcggtacgtc cctttccatc
     2941 ccaggccctt gaacgtgttc gctcgctcga gtgaccacaa acctgcttca gcgttcaaga
     3001 tgccgtcaaa ctgcatcatc gatgagagcg accacttcag attaggctga acgacgagga
     3061 gcatgtcatg ctgggccgta ccctgtgtga catgcgcgtg ccaggttcct accaggcttg
     3121 tcggatcaaa cgaagcggct ttttgaggtc cgtcttggat attagaaggg gcggggagcg
     3181 tttgcgtgag tgtcaccggg gccggcgaaa cgatggtttc agtgcggact ccacggccgg
     3241 tcctgggggg tgagccggag gttgaggttg aagc
//
LOCUS       DATMTI010000662         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_832383, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000662 DATMTI010000000
VERSION     DATMTI010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832383"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VN039_10675"
     CDS             <1..201
                     /locus_tag="VN039_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46463.1"
                     /translation="TETITVNVKESQRALTSIAEEFGTYLLELKMLNPEFKKDMLPHG
                     SYQIRVPRQTCPSRCFKQEKMP"
     gene            206..1543
                     /locus_tag="VN039_10680"
     CDS             206..1543
                     /locus_tag="VN039_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWV46464.1"
                     /translation="MRLRLSPSAARPLLQKLITAGLSTADPYQALLKHVELDRHSLIV
                     GRRAYDLSHIDRVIAVGAGKASARMAQALEAALGTRLENGLVIVKTGHTLTTKRIAVI
                     EASHPIPDRAGLQATRRLLRLTQHLTPRDMLIVLLSGGASSLLPAPVAGVTLADKQRT
                     TRLLLRSGATINEINVVRKHLSLVKGGGLAISTRARIVTLLLSDVIGDDLGSIGSGPT
                     AEDPSTFADAVGVLHRYRCWHAVPSAVRHYLHRGQKGDAPETLKSGSRRLRSEQHHII
                     GNNRMMLEAVAHAAQQAGLHTKFVSHPIMGEARVAAKQLTDLAKAITEGPGRLMRPCC
                     VVAGGETTVTVTGHGKGGRAQEFAASAACEIAGLSNTWVVALGTDGTDGPTDAAGAIV
                     SGYTVARAKKLGIDLRSSLNRHNTYPALKALKCHIYTGPTGTNVDDLYLVLLL"
     gene            1559..4186
                     /locus_tag="VN039_10685"
     CDS             1559..4186
                     /locus_tag="VN039_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWV46465.1"
                     /translation="MARPFILPLSQCSDGNLTGGKAVGLAQLLAAGFSVPQGFCITTE
                     TYIQYLLASGFREDEEWHKACALSGNERTSALTDCRTRIKQVETSQLAVQWRMALQTL
                     SLPPDQLWAVRSSATNEDTAQTSFAGLYRTHLGVSLSDIDAAVKDLWVSLWEERVVSY
                     TARQSQKTAPRMAVVIQWMVAAQSAGVAYSIHPVTGRSNQVAINAVPGLAASLVDGTV
                     NPDQYVVEVTHEGQPMRVRKRILAHHSQRQSVSKAGLHIEPFNDATHIQSSLTDAQLF
                     SLAQTTKHVEQAFGHPIDLEWAFDVGQLWLVQARPIATVRPSSNLTNDDCEWSRTNFK
                     ETLPELPSLLSLSFLERFMDRHILFQYRRLGCHIPDGLTSVRILSGRPYLNVTLFHIL
                     VAQLGGDPSVNAEQMGGEPLQTIPNVQPLNGAAFVRAVWMMWAEMRRVERSGPELFQE
                     MKDLATTYRRERILHLSVEELVLELDKLGPWLEGRDVTFGIVGGVGQCLQIFSRFLPQ
                     WLGPDWRGLLNAALQGQGTIISAQQIVRLAELTDIARDEPAVGTFLTSEPWGPAPFRE
                     ALADTKFLRTFDSYLEDYGHRAVGESDLMSPRLADTPESILAIVRVQLISGAPSQKSI
                     LRRQCNAKTTALARIKQRMGRHYHRWIIFRWCYRRLCRFFALREANRHHLMHYSTAIR
                     TLLMRLGELLVERGLLHQVDDIFFLTINDRADLLAGSTRDWKALIKARRTERERNAAV
                     EVPDTIRDWETSSEQTVTSDKSERNGVLSGTPISVGTVAGPVRLIRSVADWSRVMPGE
                     ILVVSVIDPGLAPLFGLAGGLIAEMGGTLSHGAIIAREYGLPTIGNVEGAMARLADGQ
                     RITIDAGSGTVCIESSPQP"
BASE COUNT          914 a         1233 c         1191 g          900 t
ORIGIN      
        1 accgaaacca tcacggtgaa tgtcaaggag tctcagcgcg cgctgacctc gatcgccgaa
       61 gaattcggca cctatctgct cgagctcaag atgctgaatc ctgaattcaa gaaagatatg
      121 ctccctcatg gcagctatca aatccgcgtg ccccgtcaaa cctgtcccag tcgctgtttt
      181 aagcaagaaa agatgccgta gtgctatgcg acttcgcctt tccccttctg ctgcacggcc
      241 actccttcaa aaactgatca ccgcagggct gagtacggcc gatccttacc aagccctgct
      301 caaacacgtc gagcttgatc gccactccct gattgtgggc cgtcgcgcct atgatctctc
      361 gcatatcgac cgtgttatcg cggtcggcgc cggcaaggca tcggcaagga tggcacaggc
      421 attagaggca gccttgggga caaggttgga aaatgggctc gtcatcgtca agacaggtca
      481 tacactgact accaaacgga tcgccgtgat cgaagccagc catccgattc ccgatcgtgc
      541 agggcttcag gcaacccgac ggcttctgcg cctgacacaa catctgacac ctcgagacat
      601 gctgatcgtt ctcttgtccg gaggtgcgtc aagcctgcta ccggccccag tggccggtgt
      661 gacgctggcc gataagcaac gcacgacgcg cctgctgctc cgcagcggtg caacaatcaa
      721 tgaaatcaac gtcgtgcgga aacatttgtc attggtcaaa ggtggcgggt tggccatatc
      781 cacccgggcg aggattgtta ccctcctcct ttctgatgtt atcggtgatg atctcggttc
      841 tatcggctcg gggccgacag cagaagatcc ctcgacattt gcggatgcag tgggggtgct
      901 gcaccggtat agatgctggc atgcggtacc ttctgccgta cgccactact tgcaccgtgg
      961 tcaaaaagga gatgcgccgg agaccttgaa gtccggctca cggaggttgc gctccgagca
     1021 gcatcacatc atcggcaaca accgaatgat gctggaagcg gtcgcgcacg ccgcacaaca
     1081 ggcaggcctc cacaccaagt tcgtttctca ccctatcatg ggagaagcgc gcgtggcagc
     1141 gaagcagttg acggatcttg ccaaggcgat tacggaagga cctggccgct tgatgcgtcc
     1201 atgttgcgtg gtggcgggag gcgagaccac ggtgaccgtc acgggccatg gaaagggtgg
     1261 acgtgcccag gaattcgcgg cttctgcggc atgcgagatc gccggcctct ccaacacgtg
     1321 ggtcgtggca ctgggcactg acggaaccga cggacccact gatgcagccg gggcgattgt
     1381 cagtggatac accgtcgcgc gagcgaagaa actcggcatt gatcttcggt catcattaaa
     1441 tcggcacaat acctaccctg ccttgaaagc tcttaaatgt catatctata ccggtcctac
     1501 tggaacgaat gtcgacgacc tttacctcgt cctgctgctc tagctactat gccgacttat
     1561 ggctcgtccc tttatccttc cgctgtctca gtgcagcgat ggcaatctca cagggggaaa
     1621 ggccgtcggg ctcgctcagc tcctcgccgc cggcttctct gtccctcaag ggttctgcat
     1681 cacgacggag acgtacatac agtatctgct tgcgtcaggg tttagggagg atgaagagtg
     1741 gcataaggcc tgcgcattat caggaaacga acgaacgtct gcgttgaccg actgccgaac
     1801 tcgcatcaaa caggtagaga cttcgcaact tgccgttcaa tggcggatgg cactccaaac
     1861 tctcagtcta ccgccagacc aactctgggc cgtccgatcg tcagccacga atgaggatac
     1921 ggctcaaacc agtttcgccg ggctttatcg cactcacctg ggcgtgtctc tttcagacat
     1981 tgatgctgca gtgaaggacc tgtgggtctc actgtgggaa gaacgggtgg tgagctacac
     2041 agctcgccag agccagaaga cagctccgag aatggcggtg gtgatccaat ggatggttgc
     2101 tgctcaatcc gccggagtcg cctattcaat acatcctgtg accggacgat cgaaccaggt
     2161 ggcgatcaat gccgtccccg gtctcgcagc gtccctggtc gatggcacag tcaaccccga
     2221 ccagtatgtc gtggaggtca ctcatgaagg gcagccgatg cgggttcgta agcggatcct
     2281 tgcccatcac tcccaacgac agtctgtttc caaggcggga ctgcacatcg agccgtttaa
     2341 tgacgcgact cacatccaat catcgctcac cgatgcacaa ctcttttccc tggcgcagac
     2401 gaccaaacac gttgaacagg ccttcgggca cccaatcgat ctcgaatggg ccttcgatgt
     2461 cggacaattg tggctcgtac aggctcggcc gattgcgacg gttcgccctt cctccaatct
     2521 cacgaacgac gactgcgagt ggtcgcgaac gaatttcaaa gagacgctac ccgaactgcc
     2581 gagcctatta agtctctcat ttcttgaacg gttcatggac cgccacatcc tctttcagta
     2641 tcgaagactc ggatgccata ttcccgatgg gctaacatcg gtccggatac tgagcggacg
     2701 tccttatctg aacgtgaccc tgttccacat cttggtggcc cagctaggtg gggacccatc
     2761 ggtgaacgcc gaacagatgg gcggcgaacc cttgcaaacc atcccgaacg tgcagccact
     2821 caatggagcg gcgttcgttc gggccgtgtg gatgatgtgg gcggaaatgc ggcgggttga
     2881 gcggtccggt ccagaattgt ttcaggaaat gaaggatctg gctacgactt atcgccgtga
     2941 gcgtattctg cacctgtcgg tggaagaact cgtgctcgag ctcgacaagc tcggcccctg
     3001 gttagagggc cgcgacgtca cgttcggcat tgtcggcggc gtcggccaat gcctacagat
     3061 tttcagccga ttcctgcctc agtggctcgg accagactgg aggggtttac tgaacgccgc
     3121 tctccaagga caaggaacta tcatcagtgc acaacagatt gtccgccttg ccgagctgac
     3181 ggacattgcc cgagacgaac cggcggtagg cacatttctc acatcggaac catggggacc
     3241 tgcaccgttt cgtgaggctc tcgccgatac aaaattccta cggaccttcg acagttactt
     3301 ggaggactat gggcatcgcg cagtcggtga atcggacctc atgtcccccc gtctcgccga
     3361 tacccctgaa tcgattctgg ctatcgtgcg tgttcaactc atctccggcg ccccttcgca
     3421 gaagagcatt ctccggcgtc agtgcaacgc caaaaccact gccctggctc ggattaagca
     3481 acgcatgggg cgtcactacc acaggtggat catctttagg tggtgttacc gaagactgtg
     3541 tcgtttcttc gcgttgcgag aagccaatcg acaccacttg atgcattact cgacggctat
     3601 ccgcaccctc ctgatgcgtc tcggagaact tttggtcgag cggggtctgc tccatcaagt
     3661 tgacgacatc ttttttttga cgatcaacga tcgtgccgat ctgttggctg gaagcacgcg
     3721 agactggaaa gccttgatca aagcccggag aaccgagcga gagcgcaacg cagcggtcga
     3781 ggtacctgac acgatccgcg actgggaaac ctcaagcgaa cagaccgtga catccgacaa
     3841 atctgaaagg aacggcgtct tgtcgggcac gccgatcagc gttggaacag tggctggccc
     3901 ggttcgactg atccgctcgg tggcggattg gagcagagtg atgccgggag agattctagt
     3961 ggtctcggtt atcgaccctg gattggctcc tctcttcggt cttgccggag gcttgatcgc
     4021 ggaaatgggc ggcacactgt ctcatggagc catcatcgct cgcgaatacg gactgccgac
     4081 catcggcaac gttgaaggcg ccatggcgcg gcttgctgac ggccagcgca tcactattga
     4141 cgccgggtca gggaccgttt gtatcgagtc atctccacaa ccctagggcg tgtcgtcagt
     4201 taagggattc acaaagctga gcatatgtga ttcataga
//
LOCUS       DATMTI010000663         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_279263, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000663 DATMTI010000000
VERSION     DATMTI010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279263"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VN039_10690"
     CDS             <1..422
                     /locus_tag="VN039_10690"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HWV46466.1"
                     /translation="TEQPISIPLIPDDFSSSLVGNSETMTFREIRNYLARFRHEGFSF
                     ARLLTDYYGRVAFPLVTVVMVLVGIALSLRRSGVRGGNMAVGIGQAFIVGFCYWTTHS
                     VAIALGRGGALAPMLAGWMANALFLSFGLYLLLKVRH"
     gene            500..>1615
                     /locus_tag="VN039_10695"
     CDS             500..>1615
                     /locus_tag="VN039_10695"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWV46467.1"
                     /translation="MASRVVITGLGVISPIGIGVSEFWKAALAGRSGITAIPSLGWFP
                     MSGYRSRVAGQVHNFSPEQYLSTTQASRVDRYAQFALVSTKEALTDAGLSMAKEAPHR
                     VGVIVGAGMGGMVMGEREITQLFKTQRPHRVHPNFIPTITLNSASGIVAMAHGAKGPN
                     LTISTACSSSAHALGQALQCIRSGQADVMIAVGADASITPLVFAGFCSLRALSSEFND
                     APERASRPFDRLRDGFVMGEGAATLIVESWAHAKKRKARVYAELAGYAATSEAYHMVI
                     PQEDGQEISTTMKIGLDSAGIGPDQVDYINAHATSTTIGDAVETKAIRSLFKNRADKI
                     AVNATKSLIGHTLGAAGAIGAVATTLSIHTGQIHPTAN"
BASE COUNT          334 a          446 c          471 g          364 t
ORIGIN      
        1 tcacggaaca gccgatcagc attccgttga ttcccgatga tttctcctcc tcattggtgg
       61 gaaactcaga aaccatgacg tttcgggaaa tccgaaatta tctcgcgcgc tttcggcacg
      121 aagggttctc gttcgcccgt ctgctgacgg actactacgg acgggtcgca tttccattgg
      181 tgacggtcgt gatggtgctc gtcggcattg cgctgagttt gcgacgaagc ggagtccggg
      241 gtggaaacat ggccgtgggt atcggacagg cgttcatcgt cggattctgc tactggacga
      301 cgcactccgt cgctatcgca ctaggacgag ggggagcctt ggctcctatg cttgccggct
      361 ggatggccaa tgcgctgttt ctcagcttcg ggctttatct attattgaag gttaggcatt
      421 gaagagaggt ttcctagttg actggacaat ggtcttccgg taagaaaggc gccgtgtgcg
      481 cccagaagga gggagaaaca tggcctcgcg cgtggtaatt accggtctcg gggtgatatc
      541 tcccatcggg atcggcgtca gtgaattctg gaaggcggcc ctcgcgggtc gttcggggat
      601 tactgcgatc ccttccctgg gttggtttcc aatgtccggc tatcgttcac gagttgccgg
      661 gcaagtccac aatttctcac cggaacagta tttgtcgacg acgcaggcga gtcgtgtgga
      721 tcgttatgcg caattcgcct tggtctccac aaaagaagcg ctcaccgatg ccggtttgag
      781 tatggccaaa gaggcgcccc atcgcgtcgg cgtcattgtc ggagccggta tgggcgggat
      841 ggtgatggga gagcgtgaaa tcacgcagct ctttaaaacc caacgaccgc atcgcgtaca
      901 tcccaatttc attcctacga tcacgctgaa ttcggcttca ggcatcgtcg cgatggcaca
      961 cggtgccaag ggaccgaatc tcacgatttc gacggcctgc tcctccagtg cccatgccct
     1021 tggtcaagcg cttcaatgca ttcgcagcgg ccaagccgat gtcatgatcg ccgtcggagc
     1081 cgacgcgagt attactccgt tggtctttgc cgggttctgc tccttgcgtg ctctctccag
     1141 cgagtttaac gatgctccgg agcgagcttc acgccccttt gatcgactcc gcgatggatt
     1201 cgtcatgggc gagggagccg ccacattaat cgtggaatcg tgggcgcacg ccaagaagcg
     1261 gaaagcgaga gtgtatgccg aacttgccgg atatgcggcc actagcgaag cctatcacat
     1321 ggtcattcct caggaagacg gtcaggaaat ctccacgacc atgaagatcg gtttagactc
     1381 tgccggcatc gggccggatc aggtcgacta catcaatgcc catgcgacct ctacgacaat
     1441 cggcgatgcc gtcgagacca aagcgatcag gagtcttttt aaaaatcgcg cggacaaaat
     1501 tgccgtgaat gccacgaagt cactcatcgg tcatacattg ggggcggctg gggcgatcgg
     1561 cgctgtggca acgaccctct caatccatac cggacaaatc caccccacag cgaac
//
LOCUS       DATMTI010000664         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_109583, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000664 DATMTI010000000
VERSION     DATMTI010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109583"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..511)
                     /locus_tag="VN039_10700"
     CDS             complement(53..511)
                     /locus_tag="VN039_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46468.1"
                     /translation="MNRRTIIRSIIRTASVISVMGTLCLFGDPAWAQSKNEKAFHAVA
                     VDAKNVETDVKNVIFYYEEKISETAFVPHELRELPVKRGTATVKIKFDGIKHIDLKPS
                     GSGTAPNVTITLNDGKTGEFVLAVAGSFKGQSEFGEVELPAAELKKLAFK"
     gene            complement(565..1422)
                     /locus_tag="VN039_10705"
     CDS             complement(565..1422)
                     /locus_tag="VN039_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWV46469.1"
                     /translation="MTTGELNRISVGTVPEQARGWMASVLLHLLGLVGAMLIMAKIEQ
                     PVLLEPFHWDISMVDAPVRTEPTQSESMPAERVIQPPPSPVKPKPPVRTATQPIAQQI
                     PLPTHDESTVPVETTQIVKDVVTNAEPVMESPVVESVESRPVTSQAVMAEPVASSEAT
                     TIEQRIESTETAVEQRVVQQRLVHYRRTQADYGWLGDMVRKRIEELKHYPRIARENHW
                     EGRVVVQAVIKADGTVGELSVAESSGHALLDEEALVVMRKASPLTLKHQLEKSQITIL
                     VPISYRLEG"
     gene            complement(1419..1793)
                     /locus_tag="VN039_10710"
     CDS             complement(1419..1793)
                     /locus_tag="VN039_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWV46470.1"
                     /translation="MERELNQVNVIPLVDVMLALLVIVLTTATFISTGQVPVELAKAK
                     EVNDHRDAPLMITLTADGRLFVNDQPIQADGLATVLNEHSRESAVILRADRVTVLERF
                     VGVVDEVKGLGFRQVSLEVLRS"
     gene            complement(1783..2208)
                     /gene="exbB"
                     /locus_tag="VN039_10715"
     CDS             complement(1783..2208)
                     /gene="exbB"
                     /locus_tag="VN039_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HWV46471.1"
                     /translation="MNVLIHLVDYGIIGLLVMLSLWALAVAVERWFYYRRVTPNQFEN
                     IQTMEIALTKRLVVIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGTMAVGAIMVGLSL
                     ALKATAIGLLVAIPCVVLNNILRRRVTELLTLYKVQHGA"
BASE COUNT          524 a          694 c          587 g          564 t
ORIGIN      
        1 gcagattcag aatggatcct ccttgagcaa ttgccgaccg cagtggagca acctacttaa
       61 atgccagttt tttcagctca gcagccggca attcgacttc tccgaattcg gattgccctt
      121 tgaaactgcc tgcgacggcc aagacaaatt cccctgtctt gccatcgttg agcgtaatgg
      181 tcacattcgg cgctgtgccg ctaccggatg gcttgaggtc gatatgtttg atgccgtcaa
      241 acttgatctt gactgtggca gtccctcgct tgaccggcaa ttcacgaagc tcgtggggga
      301 cgaacgccgt ttcactgatc ttctcttcat aataaaagat gacgttcttg acgtcggttt
      361 caacattctt ggcatcgacc gccacggcgt ggaaggcctt ctcgttcttg ctctgggccc
      421 aggcaggatc gccgaataga caaagcgtcc ccattacaga aatgaccgac gcggtgcgga
      481 tgattgagcg tatgatcgtc cggcgattca tcgtgattct cctttgttcg tgttccactc
      541 gacgagagtt tcttgctccg cttgttaccc ttccaagcga taactgatcg gaaccagaat
      601 ggtaatctgc gacttctcca attgatgttt gagcgtcaac ggggaggctt ttctcatgac
      661 caccaacgcc tcttcatcca gcaacgcatg gcctgagctt tcagcgacac tgagctctcc
      721 gaccgtgccg tccgctttga tgaccgcctg tacgacgact cgaccctccc aatggttctc
      781 acgtgcaatc cttggataat gttttaattc ttcaatgcgt ttcctgacca tgtccccaag
      841 ccagccgtag tccgcctgag ttctacgata gtgcacgagc ctctgttgga caacgcgctg
      901 ttccaccgcc gtttccgtag attcaatgcg ctgttcgatg gtcgtcgcct cggatgaggc
      961 aaccggttct gccatgacag cctgagaagt caccggtcgg ctttccaccg attccacgac
     1021 gggagactcc atcaccggtt ctgcgtttgt gacgacgtcc ttcacgatct gcgtcgtttc
     1081 aacaggaacc gttgactcat cgtgagtcgg tagcgggatt tgttgggcga tcggctgagt
     1141 ggctgttcga acaggcggct tcggtttaac gggtgaaggg ggcggctgga tcactcgttc
     1201 agccggcatg gactcagact gggtcggttc agtgcggact ggagcatcta ccattgagat
     1261 atcccaatga aatggttcta gcaagaccgg ttgctcaatt ttcgccatga tgagcatcgc
     1321 gccgacgagg cccaggagat gcagcaatac agaggccatc caaccacgtg cttgttcagg
     1381 aacagtgccc acactgatgc gattcaattc gccggtggtc atgatcgtag aacctccaaa
     1441 ctgacctgtc gaaatcccag tccctttact tcatcgacca cgccgacaaa tcgctccaaa
     1501 accgtaacgc gatcagctcg caatatgacc gctgactccc gagaatgctc gttcaggaca
     1561 gtcgccagcc catccgcctg aataggttga tcgttcacaa acaggcgtcc atcggcagtc
     1621 agagtaatca tcaacggcgc gtctcgatgg tcgttcacct ccttggcctt cgccagttcg
     1681 accggcactt gccccgtcga tatgaacgtg gccgtggtca aaacaatgac caagagcgcc
     1741 agcatcacat ccaccagtgg aatgacattg acttgattca gttcacgctc catgctgcac
     1801 cttgtacagc gtgagcagct cggtgacgcg ccgccgaagg atattgttga gcaccacgca
     1861 tgggatcgcc accagcaacc caatagccgt cgccttgagc gccaaactca atccgaccat
     1921 aatcgcaccg accgccatcg tccccgaggt gcccatcgta tggaacgtca acatgatgcc
     1981 caagacagtt cccaagagcc cgatatacgg cgcgttggct gccaccgttc cgattaccac
     2041 cagtcgtttt gtcagggcta tttccatagt ctgaatgttc tcgaactgat tgggcgtcac
     2101 acgtcgatag taaaaccacc gttccaccgc cacggccaat gcccataggc tgagcataac
     2161 cagcagcccg ataattccgt aatccaccag gtgtataagt acgttcatgt agaaaagatc
     2221 ctctgtttac aggatgacca gaatgttctt cttaatgccg cttaggagct ctcagtgcct
     2281 ttggccctgc caagtccata aaaaagccca tggccgcctt taggcagcca tgggctctgg
     2341 acgtaaagta ctctggcctc taaccgagg
//
LOCUS       DATMTI010000665         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_703366, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000665 DATMTI010000000
VERSION     DATMTI010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703366"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VN039_10720"
     CDS             <1..1255
                     /locus_tag="VN039_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV46472.1"
                     /translation="FAIPGGHHNLGAGRNWHFLIVPLWILNGLCYVGLLFATGQWRRL
                     IPTSWSIFPEAWDSMLTFASLSVPPESSFHPYDPLQQLVYAAVVFILGPLMILTGLAQ
                     SPALIARYPWYVALFGGRQGARSIHFIGLMLMILYVLTHLTMVVLIHFPRDLERMFSM
                     FGPSGLPSMMAFLSASLALVALIGIHIAATLYTRRHQRSFQEHAAKVVEPLMRFFFGK
                     LKSRQHFDRQNVTAMHHVNGYPPENGDYRRMAETGFLDWRLRIGGLVEQPMELSLSDL
                     QRMPKREQTVRHNCIQGWTGIATWAGVEVREVLQRCRPKLEAKWIVFHAFVQDEYAPD
                     HYYEVLRLDEVQDPQTILAYEMNGRPLPCEHGAPCRLRVETKVGYKMVKYLTHIDLVA
                     SLDQVGFGYGGFREDHQFYEKVASI"
     gene            1255..1494
                     /locus_tag="VN039_10725"
     CDS             1255..1494
                     /locus_tag="VN039_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46473.1"
                     /translation="MATSFFHEETFPTDDNTAMELVRYYYDDISAEVLTRSFHLFPAT
                     AERTFLENDRIPLADLRPDLKVVLTRFLYHRNDKK"
     gene            complement(1668..>2038)
                     /locus_tag="VN039_10730"
     CDS             complement(1668..>2038)
                     /locus_tag="VN039_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46474.1"
                     /translation="VARIMGRWVGSMALVLGLYAPNASAAEPVKINSLSTYPEMYKMK
                     VVQVEGTVRNYRMNHFIGHNTKLEKCIQAFSIEDETGIIDATYATLCGMGAVMLQNGD
                     HVTIEGHFLGVLDVRAVRKN"
BASE COUNT          426 a          595 c          505 g          512 t
ORIGIN      
        1 cttcgctatt ccgggaggac accacaatct gggagccggc cgtaattggc attttttgat
       61 cgtgccgctc tggattctga acggcctctg ctatgtggga ttgctgttcg ccacgggtca
      121 atggcggcgg ctcattccaa cgagctggtc gattttcccc gaggcgtggg acagtatgct
      181 tacattcgcg agcctcagcg tgccgccgga atcctcattt catccgtatg atcctctcca
      241 gcagctcgtg tacgccgccg tcgtttttat cctcggtccc ttgatgatcc tcacgggact
      301 cgcgcagtcg cctgcattga tcgcccgata cccctggtac gtcgctctct tcggcggacg
      361 gcagggcgca cgaagcatcc attttatcgg cctgatgctc atgatcctct atgtgttgac
      421 ccatctcacc atggttgtcc tcatccactt cccacgcgac ctggaaagaa tgttttccat
      481 gtttggtcca tcggggctgc cttctatgat ggcattcctc tcggcttctt tggcgctcgt
      541 cgccttaatc ggcatccaca ttgccgcgac gttatacacg cgccggcatc agcgcagttt
      601 tcaagagcat gccgcgaagg tcgtcgagcc gctcatgcgc ttcttcttcg gcaaactgaa
      661 gtctcgtcag catttcgacc gacagaacgt gacagccatg caccatgtca acggctatcc
      721 gcccgagaac ggcgactatc gtcgcatggc cgaaaccgga tttctcgact ggcgcttgcg
      781 catcggtgga ctcgtcgaac agccgatgga actatcattg agcgatctcc agcggatgcc
      841 gaagcgcgaa caaacggtca gacacaactg cattcaaggg tggaccggca tcgccacctg
      901 ggcgggcgta gaagtccgcg aggttttgca acgctgtcgt cctaagttgg aagccaagtg
      961 gatcgtcttc catgcgttcg tccaggacga atatgcacct gaccattact atgaagttct
     1021 gaggttggac gaagtgcagg acccgcaaac tattctggct tatgagatga acggacgtcc
     1081 tttaccctgc gaacacggcg cgccttgtcg gctgcgggtg gagacgaagg tcggatacaa
     1141 aatggtgaag tatctgacgc atatcgacct cgtggcctca ttggaccagg ttggattcgg
     1201 ctatggagga tttcgcgagg accatcagtt ttacgaaaag gtggcatcga tctgatggca
     1261 acatccttct tccacgaaga aacatttccc accgacgaca acaccgccat ggagctggtg
     1321 cgctattact atgacgacat ctcggcggaa gtcctgacac gctcatttca tctttttcct
     1381 gcgacagcag aaagaacatt ccttgagaat gaccgcatcc cgctcgccga cctccgcccg
     1441 gacctcaagg tcgtattgac gcggttcctt taccatcgca acgacaagaa gtgagcctgt
     1501 ctcatgacaa tcatcctttt cgccttgatc atcattccga ttttctggct gctgagcctg
     1561 ctctattatt tgctttcctg gatcgatttc gacaaagatc gggcatacat ttctaaatga
     1621 cccctgtctc aaagaagcca tcgacatcct ctctccaaga acttgcttca atttttcctt
     1681 accgcgcgca cgtccagcac acctaggaaa tgaccctcaa tggtgacgtg atcgccattt
     1741 tggagcatga cggctcccat tccgcagagc gttgcatagg tggcatcgat aatcccggtc
     1801 tcatcctcga ttgagaatgc ctgaatacac ttctccagct tggtattgtg gccgataaag
     1861 tgattcatgc ggtagttgcg caccgtgcct tccacctgca cgactttcat tttgtacatt
     1921 tccggatagg tggacaatga attgattttg accggctcgg ctgccgatgc gttgggtgca
     1981 tatagaccca ataccagcgc catcgatcct acccatcttc ccattatcct cgccacag
//
LOCUS       DATMTI010000666         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_196229, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000666 DATMTI010000000
VERSION     DATMTI010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196229"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..737
                     /locus_tag="VN039_10735"
     CDS             318..737
                     /locus_tag="VN039_10735"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HWV46475.1"
                     /translation="MKCPQCLTENDISAAKCSICEHAFAPQQVKSARYTSFYAEYFAF
                     RELVTPQLIKTVYIVGACFITAGGLLSILSPETLNEYEAGPILTRLGGIFVLLVGNLV
                     WRMMCEGAILLFSLHELLVSIDTRAKALVRQGKSHRQ"
     gene            complement(825..1412)
                     /locus_tag="VN039_10740"
     CDS             complement(825..1412)
                     /locus_tag="VN039_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46476.1"
                     /translation="MHIVSMMAVIALLGGCSGLQEVWEGPGAKVFRPQTIAVLPPMAS
                     QYDSAREDIQDVLAGALNRQGNIERVVSPENVTDIFQASKEAFDSLVFYFSRLEMTGQ
                     SDKESAIKLGKALNVDSFLVVRVNSWEYVRKEGDNVGRVGLSLRLIDATTGTTVWKAR
                     HERSSSYMFIKPSLKNIAKELADEMIKYMPPQAKR"
BASE COUNT          383 a          412 c          396 g          352 t
ORIGIN      
        1 caacgactga ggcagaaaac cagcaataaa caatggcatg aaataatgac gaatcaaaaa
       61 cccaccagag gaagcgctca ttaaaactga gaacaaatca ttgacgacat tctagccttg
      121 ctcaacaaga acggtttgac ctcgcatcag tcacttgacc ttctcgaacg gttgggcggt
      181 atcattgaga tgggtcagcg actggtgaag ccgattaccc actagcagac cctgccctca
      241 cctcatgcca caaggtcata tgaggcgcaa cgagcgtcat cgcatatctt atgaaccgct
      301 gactgaaggg agagcccatg aaatgcccgc aatgtttgac ggaaaatgac atatctgccg
      361 cgaaatgctc gatatgtgag catgcatttg ccccacagca agttaagtct gcgaggtata
      421 cgagcttcta tgccgagtat tttgcctttc gtgaattggt gaccccgcaa ctcattaaaa
      481 cggtctatat cgtaggagcg tgtttcatta ccgcaggagg gctcttgtcg attctatccc
      541 cggagacctt gaatgagtat gaggccggcc cgattctcac gcgcctcgga ggaatcttcg
      601 tcctactggt ggggaatctg gtgtggcgca tgatgtgcga aggagccatt ctgctcttta
      661 gtctccatga gctgcttgtc agcatcgaca ctcgggcgaa ggctctggtg cggcagggta
      721 aaagccaccg acaatgaggc cactcttctc aatttccatg cactggaata tgtggtggcc
      781 tgtcgaacga aggagagtaa gcagagagga ttccgagttc gacgctagcg cttcgcttgt
      841 ggaggcatgt atttaatcat ctcgtctgcg agttcttttg cgatattctt gaggcttggc
      901 ttgatgaaca tatagctgct ggaacgttca tggcgcgctt tccagaccgt cgtgccggtc
      961 gtggcatcga tcaaccgtag gctcagcccc actcgtccga cgttgtctcc ttccttccgc
     1021 acgtattccc acgaattgac ccgaaccacg agaaaggaat cgacgttgag cgctttcccg
     1081 agcttgatag ccgactcttt atcggattgc cccgtcatct ccagacgaga gaaataaaac
     1141 acaagggaat caaacgcctc ttttgaagcc tgaaagatat ccgtcacgtt ctcgggcgag
     1201 acgacccgtt cgatatttcc ctgacggttg agtgctccag ccaagacatc ttgaatgtct
     1261 tcgcgggcgc tgtcgtattg gctcgccatc ggcggcaaca cggcgatggt ctgcggtcga
     1321 aagacctttg cacccgggcc ttcccagact tcctgcaagc cgctgcaacc tccgagcagg
     1381 gcgatgaccg ccatcatgga tactatgtgc atggatgatc gagacagcat gggtctagta
     1441 taccattaga acgtgaatga aatagaagca gaaaccaggg caccctttgc acgaaagtca
     1501 tatccccccc cggcatccgc acggaaggaa aaccaccgta ggc
//
LOCUS       DATMTI010000667         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_661858, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000667 DATMTI010000000
VERSION     DATMTI010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661858"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VN039_10745"
     CDS             <1..461
                     /locus_tag="VN039_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWV46477.1"
                     /translation="KLRLAAAEGNLLRVKTFLGQGVKPHAADARGVTPLFSAAKSGHR
                     EVAALLLEQGAVVNPARQDGVTPLFIAVQEGHQDLVALLLEKGANVNAQARVGGVTPL
                     HVGAFRGDQEIVTLLLQHGADKNARMSSGERPVDLAQTQGHQALIPLLEP"
     gene            complement(511..1131)
                     /gene="thpR"
                     /locus_tag="VN039_10750"
     CDS             complement(511..1131)
                     /gene="thpR"
                     /locus_tag="VN039_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWV46478.1"
                     /translation="MLRAFLAVELSDDLRNRITLIQQDLRQRLGRESSKDSRITWVQP
                     SSLHLTMKFLGDTGELQIEPLREAVGRAMTGHEVVHMPIERLGVFPRLQQPRVLWVGP
                     SEQWEQGDDAKRLTTLHRAIEDCCQPFGFEPEGRPLSPHLTLARVKEGERHVGQALAK
                     SGVIDRPISLGLLAMDSIVLMKSELRPTGSVYTKLWEVRTGDSRSP"
     gene            complement(1237..>1610)
                     /locus_tag="VN039_10755"
     CDS             complement(1237..>1610)
                     /locus_tag="VN039_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46479.1"
                     /translation="QKFDSDTERRLAIILDRDAQKWFRPASGQFQISYKLGVSQHGYQ
                     PDFVAETADCIYMLEPKAKNELTASEVLAKKDAAVLWCSRATAHSLTNGGKPWKYVLI
                     PHDVIAENMTLAGLVKQFAVG"
BASE COUNT          335 a          451 c          463 g          361 t
ORIGIN      
        1 agaagctacg actagccgcg gccgagggga atcttctccg agtgaagaca ttccttgggc
       61 agggggtcaa gccccacgcg gccgatgcgc gcggcgtgac accgctcttt tcggcggcaa
      121 aaagcgggca tcgggaggtg gcggcactct tgttggaaca aggagcagta gtaaatcctg
      181 caaggcagga cggcgtcacg ccgctcttca tcgcggtaca agaggggcat caggatctgg
      241 tggctctgct tcttgaaaag ggagcgaatg tgaacgcaca ggcgcgagtc ggaggcgtga
      301 cgccgttaca tgtcggggca tttcggggcg atcaagaaat cgtcaccctc ctgcttcagc
      361 acggagcgga taagaatgcc agaatgtcat cgggcgagcg cccggtggat ttggcccaga
      421 ctcaaggcca ccaggcgctg attccactcc tcgaaccatg agggcttggt gtagcgaatt
      481 cggaggctgg tgtgcctgat tcatattaca ttatgggctg cggctatcac ccgttctcac
      541 ttcccacaac tttgtataca cggatcctgt cggtcgtagt tcacttttca tcagcacgat
      601 cgaatccatc gccagcaagc ccaatgaaat cggccggtca atgacaccgc tcttggccag
      661 ggcctggccg acgtggcgct ctccttcctt gacccgtgcc agcgtcaggt gtggactcaa
      721 cggcctgcct tccggttcga agccgaacgg ttggcagcaa tcctctatgg cacgatgcag
      781 tgtcgtcaaa cgtttcgcat catcgccctg ttcccactgt tccgatggcc cgacccagag
      841 cacccgtggt tgttgaagac gagggaacac gccgagccgc tcgatcggca tatggacaac
      901 ctcatggcct gtcatggctc gtccgacggc ctcacgcaaa ggctcgatct ggagctcgcc
      961 tgtatcaccg aggaacttca tcgtgaggtg aagggacgac ggctgcaccc aagtaatccg
     1021 actatctttt gaggattcgc ggccgagtcg ttgtctcaag tcttgttgaa tcagcgtgat
     1081 ccggttgcgg agatcgtcgc tcagttcgac ggcgaggaaa gcgcggagca tctattgcct
     1141 gcctattcca gaaatcgata gcctttggtt tgcttactca tcacttgcgg ccctatgcca
     1201 agcgacactc atagctttat cgtggcgacc tggccatcaa cctacggcaa actgcttcac
     1261 caaacctgcc agcgtcatat tctcagcgat tacatcatgg ggaatcagca catacttcca
     1321 aggtttcccg ccattggtga gagagtgggc ggtggcgcgg ctgcaccata gaactgcggc
     1381 atcttttttg gctaatacct ctgacgcggt cagttcgttc ttggctttcg gctcgagcat
     1441 gtagatgcag tccgctgttt cggccacaaa gtcaggttgg tagccgtgct gagagacgcc
     1501 cagcttgtaa gagatttgga attgtcccga tgcaggtcga aaccatttct gtgcgtcacg
     1561 gtcaagaatg atggccagtc gccgctctgt gtcggaatca aacttttgga
//
LOCUS       DATMTI010000668         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_703383, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000668 DATMTI010000000
VERSION     DATMTI010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703383"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VN039_10760"
     CDS             complement(<1..790)
                     /locus_tag="VN039_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWV46480.1"
                     /translation="MRQISNPPNPFESQHRDLLEPADTAKLMLYADESREILSRNDSP
                     DLPFRWSVNPYRGCFHACAYCYARPSHEYWGFGAGTDFESKIVVKKDAPQLLRRAFDK
                     PSWRGELIVFSGNTDCYQPIEAEYGLTRACLEVCAEYRNPVGIITKGALILRDLDVLV
                     RLHREASVLVYFSIPFSSDDVARQVEPHVPSVAKRFSAMKAVSDAGIPTGISIAPVIP
                     GLNEDDIPELLDRAYGAGARTAAYSLLRLSGSLEPVFLERMREAF"
     gene            930..2216
                     /gene="serS"
                     /locus_tag="VN039_10765"
     CDS             930..2216
                     /gene="serS"
                     /locus_tag="VN039_10765"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWV46481.1"
                     /translation="MYDLKYLRENLDHIRTSLGRRGNDVLWADIQKLSEERRAVTTQV
                     EQFRHELNKGSEEVARLRRAKEPAEAAMAAMKQLGDRIKDAEGTLRRVEEALTDIALR
                     IPNLPHASVPVGADASENVEVRRWGTIPTFSSPPRPHWEVGENLGIVDFDRAAKIAGA
                     RFSVMTGTGARLERALINYMLDLHTTQHGYREVIPPFMVNRPSMTGTGQLPKFEEDLF
                     RMKDEDYFLIPTAEVPVTNLHREEILSADCLPLRYTAYTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVAFTTPDRSYEELERLTSHAESILQGLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWLPSQQQYREISSCSNFESFQARRASIKYRPPGGRKEAKTDFVHTLNGSGLAVG
                     RTLVAILENYQQPDGSVEIPAVLRPYMGGVERITRE"
BASE COUNT          570 a          632 c          635 g          473 t
ORIGIN      
        1 gaaacgcctc tcgcatccgc tccaaaaaca ccggttccag gctgcccgac agtcttaaca
       61 ggctataggc ggcagtacga gctccggcac catacgcgcg atccaacaat tcaggaatat
      121 catcttcgtt caagccggga atgaccggtg cgatcgaaat accggtcgga ataccggcat
      181 cggaaacggc cttcatggcg gagaaccgct tggcgaccga cggcacatgc ggctctacct
      241 ggcgagccac atcgtccgag gagaagggaa tactgaaata caccagtacc gaagcctcac
      301 ggtgcaaccg aaccaacaca tccagatcac gaagaatcaa tgcacctttt gtgatgatac
      361 cgacggggtt ccgatattcc gcgcagacct ccaaacaggc acgcgtcaat ccgtactctg
      421 cctcgatcgg ctggtaacag tccgtgtttc cggaaaagac gatcagctct ccacgccatg
      481 acggcttgtc gaaggcacgc cgcagcagct gcggcgcatc cttcttcacc acgatcttgc
      541 tctcgaaatc cgttccggcg ccgaatcccc aatactcatg cgaaggacgc gcgtaacaat
      601 aagcacaggc atgaaaacaa ccccgatagg gattcacgct ccatcgaaac ggcaagtccg
      661 ggctgtcgtt acggctcaag atctctcgac tctcgtccgc gtagagcatc agtttggctg
      721 tgtcggctgg ttcaagcagg tctcgatgtt gcgactcaaa tgggtttgga ggattggaga
      781 tttggcgcat aagcctatcc tgtccctcat gcccgatcga tgtcaatgtt ctagaccagg
      841 tcgtatggtc atgatggggc aacctatagg tttcgttgac tctcgtgaac tcaggtgcta
      901 gattccaacc gctttttgaa gagcgactcg tgtacgatct gaagtatctt cgcgaaaacc
      961 tcgatcacat tcggacctct ctcggacgac gtgggaacga tgtcttgtgg gctgatatcc
     1021 aaaaattgag cgaagaacgg cgagccgtta cgacgcaggt cgaacagttc aggcacgaac
     1081 tcaataaggg ctcggaagag gtggctcgat tgcgccgagc caaagaaccg gctgaagcag
     1141 ccatggcggc gatgaaacag ttgggggatc gcattaagga cgccgaggga acccttcgaa
     1201 gggtcgagga agcgctcacc gatatcgcgc ttcgcatccc caatctccca catgcctcgg
     1261 tcccggtagg ggctgatgca tcagaaaatg tcgaggtccg tcgatggggt accataccga
     1321 ctttttcgag tccccccaga cctcactggg aagtcggcga gaacctcgga atcgtggatt
     1381 tcgatcgagc cgcgaagatc gcaggagcca gattttccgt catgaccgga acaggtgcaa
     1441 ggctggaacg agcgctgatc aattatatgc ttgatctgca caccactcag catggatatc
     1501 gagaggtgat tcctcctttc atggtgaatc gtccttcgat gactgggacg ggtcagcttc
     1561 cgaagttcga agaagatctc ttccgaatga aggacgaaga ttacttcctg attccgacgg
     1621 ccgaggtgcc ggtgacgaac ctgcatcgtg aggagatcct aagcgcagac tgcttgcccc
     1681 ttcgctacac ggcctacact ccttgcttca gacgagaggc agggtcgtat gggaaagaca
     1741 caagaggact catccggctt caccaattca acaaagtgga gctcgtagca tttacgacac
     1801 cggatcgttc gtacgaagag ctcgagcggc taacaagcca tgctgagtcg atcttacagg
     1861 gattgaatct tccctaccgc gtcatcacgc tgtgcaccgg tgatatggga ttttctgcag
     1921 cgaagacgta tgaccttgag gtgtggctgc cgtctcaaca acaataccgg gagatttcgt
     1981 cctgcagtaa tttcgaatcc tttcaggcca ggcgagcgag catcaagtat cgcccacccg
     2041 gaggaaggaa agaggcgaag accgactttg tccatacgct caatggttcc ggactggccg
     2101 tcgggcgaac actggtagct atcctggaaa attaccagca gcctgacggc tcggttgaga
     2161 tccctgcagt actgcggcca tacatgggag gagtggaacg gatcacccgc gagtaagaca
     2221 ccgagtaagg gttttatccg gcgactccat aactaaaaag cgatgaatag tcgccgggtc
     2281 aaccgaaggt tggtatggag tggccgaacg
//
LOCUS       DATMTI010000669         1815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_109697, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000669 DATMTI010000000
VERSION     DATMTI010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109697"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VN039_10770"
     CDS             <1..376
                     /locus_tag="VN039_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46482.1"
                     /translation="TLLRLLPRTQSSGSLFAPDDSIRRVDSVVDRINAKFGYNKLYFG
                     SMQLALEHDAAPMRIPFNRIPDTGSENEAIHESDASHDALWLQSLNRFKALAEGEHRR
                     REGKSNPSSQGVTESILRGRKP"
     gene            518..1234
                     /locus_tag="VN039_10775"
     CDS             518..1234
                     /locus_tag="VN039_10775"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWV46483.1"
                     /translation="MKFIDLIRTILHDKPEGATPQQIRDQIKAEYPGWYGTDVHRRNV
                     EKGHYNNLDHALLAEIYIATRQASDIYSDRATRPMTLTLESSSPVPDVVQIDDSDLTE
                     TEDLNRLEQGAGTLYVLGTNLYTKGGAEIVKIGITTGNVNDRIKQLYTTGVPTKFRVI
                     QTFDVQNYAEIEQALHKLLDPYRINRAREFFTELCLPYIDRILAIHLEIQRAKTTQSE
                     GDAQPIVAMDNPSDTRFGRR"
     gene            1250..1630
                     /locus_tag="VN039_10780"
     CDS             1250..1630
                     /locus_tag="VN039_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46484.1"
                     /translation="MKKYISLLALLLTSCAYNHVGVLNKPGNSNVFISTLPDGKPAIT
                     LYQINRKASGIMYFDEGSTAMSLPPGAYRVAIDCRRPGSLYIVDGSNWFSFAIKSNES
                     YVLDCSPLHGANNFLVRVVAKTAN"
BASE COUNT          499 a          534 c          436 g          346 t
ORIGIN      
        1 gaccctgctg cgactgctgc cgcgcacgca aagcagcggc tcgctgttcg cacccgacga
       61 cagcatccgc cgggtcgaca gcgtggtcga ccgcatcaac gcaaaattcg gctacaacaa
      121 actctacttc ggcagcatgc agctggcgct cgaacacgac gccgcgccga tgcgcatccc
      181 gttcaaccgc attcccgata ccggcagcga gaacgaagcc atccacgaaa gtgatgccag
      241 ccacgacgcg ttgtggctgc agtcactgaa ccgattcaag gcattggcgg aaggagagca
      301 tcgccggcgg gaaggaaagt cgaacccatc ctcgcaaggc gtaaccgaaa gcatacttcg
      361 cgggcgcaaa ccataacgtt gaatctgcaa acgtcatgct gagttacacc gcttgaagac
      421 gtagacttga aaccggacta tcgttgtacg catcgccgca tgaagccaaa taaacgatga
      481 gtttccagaa tcgatcaacg agggagtgtc gctgcaaatg aaattcatag acctcattcg
      541 tacgatcttg cacgacaaac cggaaggcgc tactccgcaa cagatacgag accagatcaa
      601 ggcggaatac ccgggttggt atgggacgga tgtccaccgc agaaacgtcg agaagggcca
      661 ctacaacaat cttgatcacg cacttctcgc agagatctac atcgcaaccc gacaggcctc
      721 cgacatctat tccgatagag ccacaagacc aatgacgctg actttggagt cgtcatcgcc
      781 ggtgcctgat gtggttcaaa tcgatgactc ggacctcacc gaaacggagg acctcaaccg
      841 actggaacaa ggtgctggca cgctatatgt actcggcacc aacctctaca caaaaggtgg
      901 cgccgagata gtcaagattg gtatcacgac agggaacgtc aacgatcgaa tcaagcagtt
      961 gtatacgacg ggagtcccga cgaagttccg cgtcatacaa acctttgacg tccagaacta
     1021 cgcggaaatc gaacaggcac tacacaagtt actcgaccca taccgcatca accgggccag
     1081 ggaattcttc acggagctgt gccttccata catcgacagg attctggcaa tccacctcga
     1141 aatccagcgc gcaaaaacta ctcaaagtga aggtgacgcc caaccaatcg ttgcaatgga
     1201 caatccatcc gatacccgat ttggtcgacg ctgagttcag acttttgaca tgaagaaata
     1261 catcagtttg cttgctctcc tactgacctc ctgcgcctac aaccacgtcg gcgtactcaa
     1321 caagcccggc aactcaaacg tcttcatctc aaccctgccc gacggcaagc cggccatcac
     1381 tctttaccaa atcaaccgca aggcatcagg gatcatgtac ttcgatgagg gcagcacagc
     1441 aatgtccctg cctccggggg cgtacagagt tgcgattgat tgccggcgtc caggcagcct
     1501 gtacatagtc gacggtagca actggttttc cttcgccatc aagagcaacg agtcctacgt
     1561 cctcgattgt tcacccttgc atggcgccaa caatttctta gtaagagtcg tggcaaagac
     1621 ggccaactga ccatccaagc agtcacacat tgatcaattc gcgtgtcatg gatgcaacca
     1681 caatggaata tttgtcattg ttcagaatgc caaccccgat gaagataacg ggacgatctt
     1741 caagcatcac taacgccttc atcaattcga tcattccagt cgtactgccc agtgcggaag
     1801 aagtaaagca ggcac
//
LOCUS       DATMTI010000670         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_196294, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000670 DATMTI010000000
VERSION     DATMTI010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196294"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VN039_10785"
     CDS             complement(<1..741)
                     /locus_tag="VN039_10785"
                     /inference="COORDINATES: protein motif:HMM:NF017829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HWV46485.1"
                     /translation="MRYGLVLALSLFLTAAGVHCSAAADKATAAKAGQPDDPQPLIYT
                     PPKKPTPRARVGGTLRGTEGNEPELVALVPDHVGLTVKQTPTLNWYLSKPTAYPLRFT
                     LNDTQKIRPIYEGSIPTPMEAGVQSIDLKILGLTLEPNVQYRWFVSASPNPESPSRDT
                     VAGGMIERCEFSECLTITSVNLTCDRESVRTNALIGFWYDAMGCVCSLIEKDPKDPSL
                     RRLRAALLRQVGLNGVADWDLRSIQNSGK"
     gene            complement(757..3051)
                     /locus_tag="VN039_10790"
     CDS             complement(757..3051)
                     /locus_tag="VN039_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWV46486.1"
                     /translation="MLIIWFLLSVTVQLFAVTRSESHDLSSPSGSLMKKGAQAFERGG
                     FSEALSHWKQAAELYKSAGNKPAQVEALILSSQASMALGQSKQALQSLELALALAQRS
                     GESLVEATLFGHLGRTYLTLRQLPEASDYLQQAASTARKQNSSPLLATTLNDLGILFA
                     LKQQDKEALDAFQESVIHSQSAGLLLLATTARTNAARTLLRLGQPTHSRVALDEALDY
                     LKDLPPSRDKSLGLIGIALAYERLLSQLPQERDPLLLRTVGVLQEAVTVAEERGDKRT
                     LSYALGYLGHLYETEFRLDEALQFTRRALFIAQSVDAPESLYRWQWQLGRQLAATGRL
                     DEAIASYRQAATTLQPIRVEVTHASSESPLAGQDTMKPMYFELADLLLQRASLTEDRK
                     AAEGYLLAARDAIEAYKTAELRDYFKDDCVDAVRSGLTTLDRLSSDTAVIYPIMFSSR
                     LELLMSLPTGLTRVSVPVTADRLTQEIREFRRLVEKRTTREYLPHAQQLYDWLVRPLE
                     PDLSQLQITTLVFVPDSAIRTIPLAALHDGSSFLINKFALAMTPGLTLTDPRPLNRDK
                     LRFLMAGLTKSVQGFPPLPYVADEVESIQRLYKGDQLMNNAFQSSRLERELREGGYGG
                     LHIATHGKFSTDVNDSFLLTFDGKLTMQTLDQLIGLFRFRQEPLELLTLSACQTGVGD
                     DRAALGLAGMAIKAGARSALATLWFINDEASASLISEFYRQLRNPALSKAVALQRAQL
                     KLLSDRIYEHPAYWSPFLLLNNWL"
     gene            complement(3063..>3731)
                     /locus_tag="VN039_10795"
     CDS             complement(3063..>3731)
                     /locus_tag="VN039_10795"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HWV46487.1"
                     /translation="IPGSSDTGVSAVSALRFIQEYQHRTASSVIALRSRVSVGFDVLG
                     ATINSREATTAGGEPLPSGRFVSWLGQVQAVKRLDYLGGWQILGRLDLQLSNNRLFPL
                     EQIPVGGRFSVRGYRENTLVRDNGFLVSLESRIPLLKFASGEDMLQFAQFVDLGRAWL
                     SRTSTPAPETLASVGLGLRWNVLPRDRARFELYWGVPLNHVPHPSGNLQDYGIHLQAV
                     VQAL"
BASE COUNT          797 a         1037 c         1109 g          788 t
ORIGIN      
        1 ttttccgctg ttctgaatgg agcggagatc ccagtccgct actccattga gaccaacttg
       61 cctgaggagg gccgcgcgaa gcctccgaag cgacggatcc ttcggatctt tttcaatcaa
      121 cgagcagaca cagcccatcg catcgtacca aaacccgatg agggcattgg tgcgcacgct
      181 ttcacggtca caggtcaggt tcaccgaggt gatcgtcaga cattcgctga actcgcagcg
      241 ctcgatcatc cctcctgcga cggtatctct ggagggggac tctggattgg ggctggccga
      301 cacaaaccag cggtattgga cattcggttc aagtgtaagt cccaggatct tgaggtcgat
      361 ggattgaacc cctgcctcca ttggagtggg gatggaacct tcatagatcg gtcgaatttt
      421 ttgggtatcg ttgagtgtaa agcgtagagg ataggccgtc ggttttgaga gataccaatt
      481 caacgtcggc gtttgcttca cggtcaaacc cacgtggtca ggcaccaagg cgacgagttc
      541 cggttcattg ccctccgttc cccgtaacgt ccccccgact cgcgctcgag gagtcggttt
      601 cttcggcggt gtgtagatga gaggctgcgg gtcatccggt tgaccggcct ttgcggcggt
      661 cgccttgtct gcggctgccg aacaatgcac tcccgctgcg gtcaggaaaa gactcaatgc
      721 taggactaat ccatatctca tggtgatgct ccctaatcac agccaattat tgagcaacag
      781 gaatggcgac cagtaggccg ggtgttcata aatcctgtcg ctcaacagct tcaactgcgc
      841 gcgctggagc gctacggctt tcgagagagc cggattgcgc agttgccggt aaaattctga
      901 aatcaacgat gccgacgctt catcgttgat gaaccacagc gtggccaatg cgctgcgggc
      961 cccggccttg atggccatgc cggccaggcc aagggctgca cgatcgtccc cgacgccggt
     1021 ttggcaggcg ctcaaagtaa ggagttcaag gggctcttgc cggaaccgaa agaggcctat
     1081 cagttgatcg agggtttgca tcgtcaattt cccgtcgaag gtcaggagga acgaatcgtt
     1141 cacgtcggtc gagaacttgc cgtgcgtggc gatatgcagg cctccatatc ctccttcacg
     1201 caattcgcgt tccagtctgg atgattgaaa ggcattgttc atcagttggt cgcccttata
     1261 gagccgttga atggattcga cttcgtccgc cacataggga aggggtggaa acccttgcac
     1321 agatttagtg agaccggcca tgaggaatcg gagcttgtcg cggttgagtg gacgtgggtc
     1381 ggtcaacgtc aaaccgggtg tcatggcgag cgcgaacttg ttgatcagga acgacgagcc
     1441 gtcatggagc gccgccagcg gaatcgtccg aatggcgctg tccggcacaa acaccaacgt
     1501 cgtgatctgc agctgcgaga ggtccggttc cagcgggcgc acgagccagt cgtacagctg
     1561 ttgggcatgg ggcaaatatt cacgggtcgt acgtttttcc accaggcggc gaaactctcg
     1621 gatttcctga gtcaatcgat cagccgtcac cggcaccgag acacgcgtga gcccggtcgg
     1681 caagctcatg agcaattcca accgggaact gaacatgatg ggataaataa cggccgtgtc
     1741 cgacgataac cgatccaacg tggtgagccc ggaccgcacg gcgtcgacgc aatcgtcctt
     1801 gaaatagtcc cgcaactcgg ccgttttata ggcctcgatc gcatcgcgag ctgcgagcag
     1861 atacccttcg gctgccttcc tatcctcagt gagtgatgcc cgttgcagta agaggtcggc
     1921 cagctcaaag tacatcggct tcatggtgtc ttggccggcc agaggactct cggaagaggc
     1981 gtgtgttacc tcgacccgta tcggttggag cgttgttgcc gcttgtcggt atgaggcaat
     2041 ggcctcgtca agccggccgg ttgcggccag ctgtcgcccc aactgccatt gccatcggta
     2101 gagcgattcc ggtgcatcca ctgattgcgc gatgaacaat gcccgtctgg tgaattgcaa
     2161 cgcctcgtcc aggcgaaatt ctgtttcata cagatgacca agataaccaa gagcatagga
     2221 cagggttctc ttgtctcccc gctcctctgc gaccgtgacg gcttcctgaa gtacgcccac
     2281 ggttcgcaat agaagaggat ctctttcttg aggcagctga gaaaggagtc gctcataggc
     2341 cagagcaatg ccgatcaagc cgagtgattt atcacgagat gggggcaggt cctttaaata
     2401 gtcaagcgcc tcgtcgaggg ctacgcgact gtgagttggt tggccgagtc gtagcagggt
     2461 gcgtgcggcg ttcgtacgag cggtggtagc gagcaggaga agtccggcgc tctgtgagtg
     2521 gatgacgctc tcctgaaatg cgtccagtgc ttctttgtcc tgctgcttca acgcgaaaag
     2581 aatgccgaga tcgttcaggg tggtggccag gaggggcgaa gagttttgtt tccgcgcggt
     2641 cgacgccgct tgctggagat agtccgatgc ctcgggaagt tgccggagcg tcaggtacgt
     2701 ccgtcccaga tgtccgaaca gggtggcttc cacaagcgat tcaccacttc tctgcgcaag
     2761 cgccagcgcc agttcaaggg attggagcgc ttgtttggat tgccccaacg ccattgaggc
     2821 ctgagatgag aggatgagcg cctcgacctg tgccggcttg ttgccggcac ttttatacag
     2881 ttccgcagcc tgtttccaat gagacaacgc ctcactgaag cctccacgct caaaagcctg
     2941 agcacctttt ttcatcaacg agccggaagg agaagagagg tcatgtgatt ccgatcgtgt
     3001 gacggcgaac aattgaaccg tcaccgacaa gaggaaccag atgatcaaca ctgtgtatct
     3061 catcacaagg cttgcacgac tgcttggaga tggatgccat aatcctgcag gttgccggac
     3121 ggatggggta cgtgattgag cggtaccccc caatagagtt caaatctggc tcggtctcgg
     3181 ggcagcacgt tccaacggag accaagaccg acgctggcca gcgtctctgg agccggagtg
     3241 ctggtgcgcg acagccaggc tcgtcccagg tcgacgaatt gggcaaactg aagcatatct
     3301 tcaccgctag cgaacttcag gaggggaatc cggctttcca gcgaaacgag gaaaccgttg
     3361 tcccgcacca gcgtgttttc ccgatagccg cgcacgctga accgtccgcc caccggaatc
     3421 tgttcaagcg gaaacaatcg gttattgctc aactgaagat ccagccgtcc tagaatctgc
     3481 catccgccca aatagtccag ccgtttgacc gcctgcactt gtccgagcca cgatacgaag
     3541 cggccgctcg gcagcggctc cccgccggca gtggtcgcct cgcgcgagtt gatcgtcgcg
     3601 cccaggacat cgaaaccgac gctgacgcga gaacggagag cgatcacgga cgaagcagtc
     3661 cggtgctggt actcttggat gaaccgaagc gcgctgacgg cgcttactcc ggtatcagac
     3721 gatccgggaa t
//
LOCUS       DATMTI010000671         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_703437, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000671 DATMTI010000000
VERSION     DATMTI010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703437"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VN039_10800"
     CDS             <1..567
                     /locus_tag="VN039_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HWV46488.1"
                     /translation="SQHLSGIASLTQRFCRAVRGYPVSILDTRKTLPGWRALQKWAVS
                     LGGGINHRRSLSDGVLIKDNHLTLLQRNRRPVERACRLARVHRSSALPIIVEVESLSE
                     VRQALAGKPDIILLDNMAPDLVRRAVALIKKQALVEVSGGITLKNVRVMAAAGADRIS
                     IGALTHSAPAASVSLVMTLAQPSRRRRA"
     gene            571..1410
                     /locus_tag="VN039_10805"
     CDS             571..1410
                     /locus_tag="VN039_10805"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWV46489.1"
                     /translation="MVSSTPLSLDGIRSTLATTSLGHALYLHQDLPSTNREALSLAQN
                     GAQDGTVVIAESQSGGYGRHGRAWFSPPAMNIYCSVIVRGMGGHLSLSQWLSWVPLVS
                     ALAVTEAVQQMAAVSLSLKWPNDLLLHERKVGGILCESSVTTTNDPVVVIGIGLNVNV
                     PPMSFPEELRPIAASLMEASRRPIDRNRLIAQLLLELEHCLGELRTHGPVRLRQVYTA
                     RCTTLGRQVRVLFANEQPIVGIAEALSADGALQVRTLSAHPRSQPMPLVDVRAADVIH
                     LRE"
     gene            1435..>2137
                     /locus_tag="VN039_10810"
     CDS             1435..>2137
                     /locus_tag="VN039_10810"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWV46490.1"
                     /translation="MNPMLLAIDIGNTNVVAGIFDGLTLLTHWRLATDPKTTADEYGV
                     LCLNLMARDGRVPEQITGAIISSVFPALTETFESMVETAFGCIPITVSSDMDTGLTLK
                     YSNPKEIGSDRIVNAAAAYEKFHRDLIIVDFGTATTFCAVNRDGEYLGGVIAPGLTIS
                     ADALFTRAAKLSKVELARPKTVIGTDTAGSIQSGLIFGYAGLVDTLIQRMEGEMGRTS
                     YVIATGGLASVIAPEA"
BASE COUNT          444 a          638 c          587 g          468 t
ORIGIN      
        1 tctcaacacc tgtccgggat cgcctcctta acgcaacggt tctgccgggc cgtgcgcggc
       61 taccctgtca gtatcctgga tactaggaaa actcttcccg gctggcgtgc gcttcaaaag
      121 tgggcagttt cgctcggcgg agggattaac caccgacgat cattgagtga cggtgtttta
      181 atcaaagata accatctgac cctgctccaa cgcaatcggc gaccggttga acgggcatgt
      241 cgattagcac gtgttcatcg atcaagcgcg cttccgatca ttgttgaagt cgaatctctc
      301 tccgaggttc ggcaggcact cgcaggaaag cctgatatta ttctgctgga caacatggcg
      361 ccggacctag tccgacgtgc cgtggcattg atcaagaaac aggccctggt ggaagtgtcg
      421 ggcggcatca cgctgaagaa tgtccgagtc atggcagcag ccggcgccga tcgcatctcc
      481 atcggagcac tcacccattc cgcccctgca gcatcggtca gccttgtcat gaccttggct
      541 cagccttcac gacgccggcg tgcgtagttg atggtgtcct ccaccccact cagcctcgat
      601 ggtatccgca gtacccttgc gaccacgtca cttggtcacg ccttgtatct ccaccaagat
      661 ctcccctcga ccaacaggga ggcattgtcg cttgctcaga acggcgcaca agacggcacc
      721 gtcgtcattg ctgaaagcca gtcaggcggc tacggccgac atgggcgcgc ctggttctct
      781 ccgccggcaa tgaacatcta ttgctccgtc attgtacgcg ggatgggggg acacctttcc
      841 ctttcacaat ggctatcctg ggtgccgctt gtcagcgcac tggccgtgac cgaagccgtt
      901 caacagatgg cggctgtgtc cctttcgctg aagtggccga atgatctcct gctccatgag
      961 cgcaaagtcg gcgggattct ctgcgagagc agcgtcacca caaccaatga tccggtcgtg
     1021 gtcattggaa tcgggctcaa cgtgaatgtt cccccaatgt cttttcctga agaattgcgg
     1081 ccgattgcgg catcattgat ggaagcctcg cgccggccga tcgaccgaaa tcgactcatt
     1141 gctcaactgc ttctggaact tgaacattgt cttggcgagc ttcggactca tggaccggtc
     1201 cggttacgac aggtctatac ggcccgctgt accacgctgg ggcgccaagt tcgcgtcctg
     1261 ttcgcgaatg agcaaccgat cgtcggcatc gcagaagctc tctctgccga cggcgcgttg
     1321 caagttagaa ccttgtcagc tcaccctcgc tcgcaaccga tgcctctcgt cgatgtccgg
     1381 gcagccgatg tcattcacct gagagagtag cgaaagagca ccaggccggg taaaatgaat
     1441 ccgatgctct tagccatcga catcgggaat accaacgtcg tcgcaggaat ctttgacgga
     1501 ttgactctct tgacccattg gcggttggcg accgatccca aaaccactgc cgatgagtat
     1561 ggcgtccttt gcctcaacct catggcccga gacgggcgcg tccctgagca gatcaccggt
     1621 gcgatcatct ccagcgtctt tcccgccctc acggagacgt ttgaatcgat ggttgaaacg
     1681 gcctttgggt gcatccccat caccgtctca tccgacatgg ataccggctt aacactgaaa
     1741 tactcgaacc cgaaagagat cggaagcgac cgcattgtga atgcggcggc cgcctatgag
     1801 aagtttcacc gcgatctcat catcgtcgat tttggtacag caacgacgtt ttgcgcagtc
     1861 aaccgagatg gagaatatct tggaggcgta atcgcaccgg gcctgactat ttccgccgac
     1921 gccctgttta cacgggctgc aaaattgagc aaagtcgagc tcgctcggcc taagaccgtc
     1981 attggaaccg atacagccgg tagtattcag tcggggctca tcttcggcta tgccggtctc
     2041 gtggacactc tgatccagag gatggaagga gaaatggggc gcacgtcata tgtgatcgcc
     2101 acgggtggat tagcctcagt gatcgcgccg gaggcgc
//
LOCUS       DATMTI010000672         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_323032, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000672 DATMTI010000000
VERSION     DATMTI010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323032"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VN039_10815"
     CDS             <1..282
                     /locus_tag="VN039_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46491.1"
                     /translation="DQIGGEGEREKGGQRRSSHGREVAEAAGEAAMTDRFRRVKVAAE
                     VPSFKGKVCGDKDFGVRRRAQNSTIVADAKGDRPTSRGAEVAADLFDQG"
     gene            543..>1528
                     /locus_tag="VN039_10820"
     CDS             543..>1528
                     /locus_tag="VN039_10820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWV46492.1"
                     /translation="MSLKKRYYIMFVSRDEDGSLNKVPVPLHYAYIFVAAAVIGMFTI
                     TGMAGSYSRMLIKTARFNQLRQDHNSLQKDYAHLEKTAHDKDVEAASLGSLASEVSAL
                     YGLTAGKLTSTGKVVMTAGRHRKSSGTESVATTPLTETSNSLTDESYYKSLQSFYALR
                     NSAMSGSVTRALSVIGGPGYASSSSMDMTDTGNLQMASYAPSLWPVMGPITSSFGERE
                     DPVKGNGEGEFHKGIDISAPNGTPVHATADGVVKLVGMVNGYGREVIIDHGHGVESCY
                     AHLSAFASIVGQVVIRGQVIGYVGHSGRVTGPNLHYEVRIRNTPVNPHRYMRV"
BASE COUNT          328 a          390 c          489 g          321 t
ORIGIN      
        1 gaccagatcg gcggggaggg cgagcgagag aaaggcggtc aaaggcgtag ctcccatggc
       61 cgcgaggtcg ctgaggccgc gggcgaggca gcgatgaccg accgattccg gagggtgaag
      121 gtcgcggcgg aagtgccgtc cttcaagggt aaagtctgtg gtgacaagga cttcggagtt
      181 cgtcggaggg cgcaaaatag cacaatcgtc gccgatgcca agggcgaccg tccgacttcg
      241 cgaggtgctg aagtcgcagc ggatttgttc gatcagggct aactctctca tggcgggccg
      301 aatatggtcg aaaagtggta tgacgatggt atattgtgtg cctaaatcga gttgtgcggc
      361 atccggtggt ttgttaggct gtaagagacg cccgaactaa aactgcaatt cctgaatgac
      421 gattggtaaa tctcatttgc cttagttgat tgagttgaat tcgtccctcg agttcaacag
      481 ctgggaagtt ctgtgagcgg gattgcagtg cggtcaaaat tcatctgggt ctggattgtc
      541 gattgagcct caagaagcgt tattacatca tgttcgtcag ccgtgacgaa gacgggagcc
      601 tgaacaaggt tccggtgccg ttgcattacg cctatatctt tgtggcggcg gcagtcatcg
      661 gtatgtttac gattaccggg atggcgggat cgtactcgcg gatgctgatc aagacggcgc
      721 ggttcaacca gctgcgacag gaccacaact cgctgcaaaa agactatgcg catctcgaga
      781 agacggcgca cgataaggat gtcgaggcag catcgctcgg atccctggcc agcgaggtct
      841 cggcgctcta tggcctgact gcgggcaaac tgacctccac aggcaaggta gtgatgacgg
      901 ctgggcggca tagaaagtcc tcgggcaccg aatcggtcgc gacgactccg ctgaccgaga
      961 ccagcaacag cttgaccgac gagtcgtact acaagtcact gcaatcgttt tatgctctgc
     1021 gcaactccgc gatgagcggc tcggtgacac gtgcgctgtc ggtgatcgga ggccccggct
     1081 atgcttcctc aagcagcatg gacatgaccg ataccggaaa cctccagatg gcctcttatg
     1141 ctccttcgct ctggccggtc atgggaccga ttaccagcag cttcggcgag cgcgaagacc
     1201 cggtgaaagg caatggcgag ggcgagtttc ataaaggcat cgacatctcg gctcccaacg
     1261 gcacccctgt ccacgcgact gccgatggag tcgtgaagct ggttggaatg gtgaatggat
     1321 atgggcgcga ggtgatcatc gatcacggcc acggtgtcga gagctgctat gcgcacctgt
     1381 cggccttcgc ttcgattgtg ggccaggtgg tgattcgcgg gcaggtgatc gggtatgtcg
     1441 gtcacagcgg acgggtcacc ggtcctaatc ttcactatga ggttcggatt cgcaatacgc
     1501 cggtcaaccc gcatcgctat atgcgtgt
//
LOCUS       DATMTI010000673         4812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_746782, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000673 DATMTI010000000
VERSION     DATMTI010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746782"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..574
                     /locus_tag="VN039_10825"
     CDS             182..574
                     /locus_tag="VN039_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46493.1"
                     /translation="MAEVNSMYHAQTRTDALRSQIEKLEGLAHHTVVNPAFEQFDTET
                     EDLLSTIYGADHRYVEAYKYATVGEAEALVNLPESAQEPLTRDIPKTGLQQRRQVLQG
                     ILTELQELDAQEAAVLTGEDREDPPGPS"
     gene            complement(646..876)
                     /locus_tag="VN039_10830"
     CDS             complement(646..876)
                     /locus_tag="VN039_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46494.1"
                     /translation="MLFPQIPADKWWLRGVIARSVGIVAVALGFVLGMYGLDHPTSPW
                     LPTALGLLVTGMVAQGYGLYCSIKRMQQLNSR"
     gene            1198..3708
                     /locus_tag="VN039_10835"
     CDS             1198..3708
                     /locus_tag="VN039_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46495.1"
                     /translation="MLIAAIAVVIVVTWLAAWQYVQQTLIRTAGEALSLGAAEIASKF
                     DRMVFERYGDLHAMAVVFSNRMKDDPAFVQRYLDTVRDTYETYHWLGVADKEGRLVGT
                     TSAESPSGTDVSGTPWFREVRARAAGRPDAPLLGGVDAFLTEKGAPDAIAISLAIYDS
                     RGTFAGVVTSRIAIPDLEAIAVETLHGLQNTNSTLTNLEYQIIAEDGVAYVDSDLAHK
                     GRSNFFTLKLQSLELARQGSVGYIEEMNLRWHIPVITGYSRTRRWDGPDAFQWTVLVR
                     IPTASLVQPVQSFHLKVAAVGVAIVAPIFYLLIWMQARLRKEWQLAQAEHLRAMAAEA
                     QYHLLLQTTDQGIFGLDLQGRCTFINRAAAKMLNYTPDDVLGHPFHDRVHDGNVSCND
                     RCVLVQALSHGCSTRLAEQVFRRKDGTVLEVECSVFPVTEDSRTTDFVFTFMDITERK
                     QRTQALLQYQGRLQSLATQLREADELARQRLATELHDNLAQTLALCRMKLAALSCATT
                     PSIAAGIAPVTGLLKEALSVTRQLMSDLRPPMLGGDGDLAAAVQWVTAKLQRHGLTVR
                     IVDDGQRKLLDPDVLRIAHQSLHELLFNVLKHAQTTEATVRLRRIGGYLAIQVKDSGA
                     GFRMEAAQAPTQEGGFGLFNLREQLGTIGGHIRVSSVCGRGSRVTILLPLRTPSAVTP
                     LVGHQEDQTAAAAEPGRAEDHPIHIRILLVDDHQIMRQGLRSMLESEPGFEIAAEAMD
                     GEMAVEFAASIRPDVVLMDINMPKLNGIEATRRIKHLYPDIAVIGLSMHDDPKMEQLM
                     YEAGASVYLSKGTAFNIVCDTIRQVQSGDRTAQNYPVS"
     gene            3768..4076
                     /locus_tag="VN039_10840"
     CDS             3768..4076
                     /locus_tag="VN039_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HWV46496.1"
                     /translation="MKLTRSAVERLKTLIHEHPEDPIVRVQVKDLDEQRLSFSITLED
                     RVQSNDEVQIVDSLTVAIPSASALRMNGITMDYQEPDGFKFHHSDQQNDPLLNIISLN
                     "
     gene            4365..>4812
                     /locus_tag="VN039_10845"
     CDS             4365..>4812
                     /locus_tag="VN039_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46497.1"
                     /translation="MESTETTSTQDLREKTDRSLEEERGKTDEYLTQPFQEVEEKTHE
                     KVHSIRDAVDQAKESQRDALDDDKERFHAIADAPLSQVDDELLAEERERFDKLQATER
                     EAVDRVRAEERAQKESLVNAILEHERQQTDSHLLDERIHADRELITR"
BASE COUNT         1110 a         1395 c         1374 g          933 t
ORIGIN      
        1 gtcccaatga ttgaaaggct ccatactcga cagagagaag acactgacga agaacaaact
       61 attatcttcg agtgcctgcg gtgcggccat accgaatacc agttctttat tccatcgttt
      121 tggaggcgat tggcggctta gctggatcac catggtttcc aacagggaag gagatctcgt
      181 aatggctgaa gtcaattcca tgtatcatgc gcaaactcgg accgatgcgc tcaggtcgca
      241 gatcgagaag cttgaaggtc ttgctcacca cacggtggtg aatcccgcct tcgagcagtt
      301 tgataccgag acggaagacc tcttgagcac catctacggc gccgatcatc gctacgtgga
      361 agcatacaaa tatgccaccg taggcgaggc cgaagcgttg gtcaatttgc cggaatccgc
      421 gcaagaaccc ttgacgcgag acatccccaa gacgggactt cagcaacgcc ggcaggtcct
      481 tcagggtatt ttgaccgagc tgcaggaact cgacgcgcaa gaagctgcgg tgctcacagg
      541 ggaagatcgt gaggatcctc cgggccccag ctaggccggc ctaagtctcc tcttcgccgt
      601 gcgccgacag acgaccgcct caaacgtacc gagtcaatga aggcctcatc tggagttcag
      661 ttgctgcatc cgcttgattg agcagtaaag cccatagcct tgggccacca tcccggtcac
      721 gagtaaccca agcgccgtcg gaagccaggg cgaggtaggg tgatcgagtc cgtacatgcc
      781 gaggacaaat ccaagagcaa cggcgacaat gccgactgat cgcgcgatga cgccccgcag
      841 ccaccactta tctgccggga tttggggaaa cagcatatct tcagtctacc ggtgaatgga
      901 aagagagtgc aatcgccaac gagcggacag ccattacgac ggcatcacca tggattatca
      961 agcgccggga tggtttcacg tttcaccaca gcgatcagtc gcataatcat ctccggctcg
     1021 atctcatcaa cttgaactga ccgacatccc atccgctgaa acgccggacc tgttgcttca
     1081 tgacgtgact agggattctc ctaggtggaa taatggcgca attcccatat atgtcctccg
     1141 cttaacccct ccgctttatc catgaaaact tccaagcccc ttctcttcca actgcccttg
     1201 ctgatcgcgg caatcgctgt ggtgatcgtg gtcacctggc tggcagcttg gcagtatgtc
     1261 caacagacgc tgattcgaac agcgggggaa gcgctcagct tgggggcggc ggaaattgca
     1321 tcgaaattcg accggatggt cttcgagcgc tatggcgatc tgcacgccat ggcggtcgtc
     1381 ttctcgaaca ggatgaaaga cgatccggca ttcgtgcagc gctatctcga tacggtccgg
     1441 gacacctacg agacgtacca ttggcttggg gtggcggaca aagaggggcg tctggtgggg
     1501 acgacttccg ctgaatcacc gtcaggcacg gatgtcagcg gtaccccctg gtttcgggag
     1561 gtccgcgcac gggcagcggg ccggccggat gcgccgctgc tgggaggagt ggatgctttc
     1621 ctgaccgaga aaggcgcgcc ggacgcgatc gccatttcat tggctatcta tgattcgcgt
     1681 ggaacctttg ccggcgtggt cacgagccgc atcgccatcc cggacttgga agccatcgcc
     1741 gtggaaacgc tccacggttt gcagaacaca aattctacgt tgaccaacct cgagtaccaa
     1801 atcatcgccg aggacggggt ggcctacgtc gacagtgact tggctcacaa gggccggtcc
     1861 aatttcttca ccctgaaact gcagtcgctc gaactggccc gccaaggttc ggtcggttat
     1921 atcgaggaaa tgaacctgcg ctggcacatt ccggtcatca ccgggtacag tcgcaccagg
     1981 cggtgggacg ggcccgatgc gttccagtgg acggtgctgg tgcgcatccc gacggcatcg
     2041 ctggtgcagc ccgttcaaag ttttcatctc aaggtcgcgg cggtgggggt cgccattgtc
     2101 gcgccgatct tctatctgct catctggatg caagcacggc tgcgaaagga atggcagctc
     2161 gcccaggcgg aacacctgcg cgccatggcg gccgaggcgc agtatcatct tttgttgcag
     2221 accacggatc agggcatctt cggattggac ctccagggcc gctgcacctt catcaaccgg
     2281 gcagcggcca agatgctgaa ctatacacct gacgacgtgc tggggcaccc ctttcacgac
     2341 cgggtgcacg acggaaacgt atcctgtaac gaccggtgcg tgctggtgca ggctctctct
     2401 cacggatgtt ccacgcggct cgccgagcag gtgtttcgtc gcaaggatgg gaccgtactg
     2461 gaagtcgaat gctctgtctt tcccgtcacg gaagacagcc gcacgaccga cttcgtgttc
     2521 acgtttatgg acattaccga gcgtaagcag cggacccagg cactgctgca gtatcaaggg
     2581 cgtctacaat cgcttgcgac ccagctgcgc gaggcagacg agctggcccg ccaacgcctc
     2641 gcgaccgagc tgcatgacaa cctggcgcag acgctcgcac tctgccggat gaagctggcg
     2701 gctttgtcat gcgccacgac gccctcgatc gcggcaggca tcgcgccggt cacggggctg
     2761 ctcaaggaag cgttgtcagt cacccggcaa ttgatgagcg atctgcgacc gccaatgctt
     2821 ggcggcgatg gggatctggc tgcggcggtc caatgggtga cggccaagct ccagcgccat
     2881 ggactgaccg tccgcatagt ggacgacggc caacggaagc tcttggatcc cgacgtgctg
     2941 cgcatcgcac accaatcact ccacgagctg ctcttcaatg tgctcaagca cgcgcagacc
     3001 accgaagcaa cggtgcggct gcgccgaatc ggcggctatc tcgccattca agtcaaggat
     3061 agcggcgccg gttttcgaat ggaggctgcg caggcaccta cccaggaagg agggttcggt
     3121 ctgttcaacc tgcgcgagca actgggcacg atcggcggac acatccgcgt ctcgtcagta
     3181 tgtgggcgcg ggtcccgggt gaccattctg ctgcccttgc ggaccccatc ggcggtcaca
     3241 ccgctcgtcg ggcatcagga ggaccaaacc gccgctgcag ccgagcccgg cagagcggaa
     3301 gaccatccca ttcacattcg tattctcttg gtggacgatc atcaaatcat gcggcaaggt
     3361 ctgcgcagca tgctcgaaag tgaaccggga ttcgagattg ccgccgaagc gatggacgga
     3421 gaaatggcgg tggagttcgc agcgtccatc cgtccggacg tcgtcctgat ggacatcaac
     3481 atgcccaagc tcaacggcat agaagccacc cgccgcatca agcacctata cccggacatc
     3541 gccgtgatcg gcctgtcgat gcacgatgat ccaaagatgg agcagctgat gtacgaggcg
     3601 ggtgcctctg tctacctatc gaagggcacc gcatttaata tcgtctgcga cacgatccgc
     3661 caggtccaga gcggtgaccg cacggcgcag aactatccgg tgagctgatg ttagactgaa
     3721 tgcgaacggt caatcattct agtgatgaat agatcatgga atagaccatg aaactcacac
     3781 gatcggccgt cgaacgactg aagacgttga ttcatgaaca ccccgaggac cccattgtga
     3841 gggtgcaagt gaaggatctg gacgaacagc ggctgtcctt cagcattacg ttggaggacc
     3901 gggttcagtc caacgacgaa gtgcagatcg tcgacagtct gacagtggcg ataccttccg
     3961 ccagtgctct gcgcatgaac ggcatcacca tggactatca ggagccagac ggattcaaat
     4021 tccaccacag tgatcagcag aatgatcctc tgctcaacat catcagctta aactgatgcg
     4081 gcatttagaa gttctggtcc tgccgcgctg ccatccggct gcggcgggct ctgcaaacag
     4141 cgcgcgcctt cctaagcctt ttccttgcgc tgatactttt cgtgaactta ccacacatga
     4201 gagtaattag aataaacgct tgcaggcgaa gctcgccgcg ttgttgacat gttggaacga
     4261 gggcacctac tatactgcgc cgccaagaaa gctgaaacat cctagttccc tgcattgatc
     4321 tgattccgcc attgccagag catcaaaagg agaggtctcg atccatggag tctacggaaa
     4381 caacatccac tcaagacctg cgagagaaga ccgacaggag tctagaggag gaacgcggca
     4441 agactgatga atatttgacc caaccgtttc aagaggtcga ggaaaagacc catgagaaag
     4501 tccattcgat tcgggatgcc gttgatcagg ctaaagaaag tcaacgcgac gccctggacg
     4561 acgacaaaga acgcttccat gcaatagctg atgcccccct ttcacaggta gacgatgaac
     4621 tgttggctga ggagcgcgaa cgatttgaca aattacaagc gactgaacga gaagccgtag
     4681 atcgcgttcg cgcagaggaa cgcgctcaaa aagaatcgct cgtcaacgca atccttgaac
     4741 atgagcgcca acaaaccgac agccatctcc tcgatgaacg cattcacgcc gacagagaac
     4801 ttattacaag gg
//
LOCUS       DATMTI010000674         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_196517, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000674 DATMTI010000000
VERSION     DATMTI010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196517"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..>1549)
                     /locus_tag="VN039_10850"
     CDS             complement(1..>1549)
                     /locus_tag="VN039_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWV46498.1"
                     /translation="GLLWAGQFWRIMIEGIVIGCVVSMIGALGPSLDAGRTTTVRALA
                     PGDYEASRQLRVGMLGVAGLSLLAAAGLLSLPGPIGEVPVLGYAATLCLLAGLACLAP
                     ICVTGWRRPRRYVGREFGVRGVMRGIAVEHASRSPGRNGVTVSALMVGLAIMIGVLVM
                     VRSFRHTVELWVTDTVLADLVVAPSMWLRGTEIGSAGRSLPPMWLDFLSSIPEVAAVD
                     SYRDVRVEVNGQRVAVVSRDLRLHAQWSRYLVRKGDSSEQLRRAADTRGLLVSEVLAN
                     RLGVEEGSTIEIMTPNGPMRFPIVAVFYDYSTDGGKLLMDRALYQSLWHDDLVTVFPV
                     YLRDGSSIDRVRERITEQLSGAAGGGLPPLVISNTELRKEILEIFDRTFLLTYVLEAI
                     AVVIAMLGIVNTLVTSVLERRREFATLRAIGGSAEQIRQLVLWEAAYLGVVGIALGLI
                     GGGLLSLLLIKVINKQSFGWTIQMIVPVGALIQAVVLAVIATLVAGYFPARWAARQPI
                     VEGLREE"
BASE COUNT          373 a          514 c          387 g          275 t
ORIGIN      
        1 ctattcttcc cgcaacccct ctacgatcgg ttgccgtgca gcccagcggg ctgggaagta
       61 gcccgccacc aaggttgcaa tgactgcaag cactacggcc tgaatcagcg caccgaccgg
      121 aacgatcatc tgaatggtcc atccgaatga ttgtttattg atgaccttga tcagtaatag
      181 agaaagcaag cctccgccga taagccccaa ggcaatcccc accacaccga ggtaggccgc
      241 ctcccataac acgagctgcc gaatctgctc cgcgctgccg ccgatggccc gcaaggtcgc
      301 aaattcacgg cgacgttcga ggacggacgt gaccagtgta ttgacgatcc cgagcatggc
      361 aatcacgaca gcgatggcct ccagcacgta ggtcagaaga aatgtgcgat cgaagatttc
      421 aagaatttct ttccgtaact cagtgttact gatcacaagc ggcggaagcc ccccgccagc
      481 tgcgccgctc agttgttccg tgatccgttc tctcacacga tcgatacttg acccgtcgcg
      541 caagtatacg gggaataccg tgaccagatc atcgtgccag agcgactggt agagtgcccg
      601 atccatgaga agcttgcccc cgtccgtcga atagtcatag aacacggcca cgatcgggaa
      661 tcgcatggga ccgttcggcg tcataatttc aattgtcgat ccttcttcca cgccgagccg
      721 attggccaag acttccgata ccaagagacc acgagtatct gccgcccgtc tcagctgttc
      781 cgaagaatcg cccttgcgca cgagataccg gctccattga gcatgcaatc gcagatcccg
      841 cgacacgaca gccacccgtt gaccgttcac ctccacccgt acatcgcgat aactgtcgac
      901 cgcagccact tcaggaatgg aggagaggaa gtccaaccac attggcggga gactccgacc
      961 ggcactcccg atctctgtgc cccgcagcca catcgaaggc gccacgacca gatcggccag
     1021 gaccgtatcg gtgacccaga gttcgaccgt atgtcgaaaa cttctcacca tcacaagcac
     1081 gccgatcata atcgccaatc ccaccatcaa ggcagagacg gtgactccgt ttcgtccggg
     1141 actgcgggat gcatgctcta ccgcaatccc cctcatgact ccccgtactc caaattcacg
     1201 ccccacatat cggcgtggac gacgccaccc agtgacgcaa attggggcca gacaggccaa
     1261 ccctgccagt agacataatg tagccgcata acccaagacg ggaacctcgc ctatcggtcc
     1321 aggaaggctc aaaagtccag cggcagcgag caagctcagt cccgcgacac caagcatgcc
     1381 cacccgtagc tgacggcttg cttcatagtc accgggagcc aacgcccgga cagtcgtcgt
     1441 gcggccggca tccaggcttg gaccgagggc accgatcatg gacacaacgc acccgatcac
     1501 aattccctcg atcatgattc tccagaattg gccggcccag agtagccct
//
LOCUS       DATMTI010000675         1802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_789945, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000675 DATMTI010000000
VERSION     DATMTI010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789945"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1576
                     /locus_tag="VN039_10855"
     CDS             209..1576
                     /locus_tag="VN039_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2-like element ISGsp3 family transposase"
                     /protein_id="HWV46499.1"
                     /translation="MKHLTTEWPLLKELEEQLVRTLQKVFAVLLAALLEEIDQQLAEA
                     RDKRRYQLKDKRPTTIQTLFGEVTFRRNYYYDRQTGNYTFLLDAELGFDGARSISPCL
                     EETAVELAVECSSYRKAARTLESIVGYAVMSHEAIRQLVLEAPVSLHRPVSQRHGRVL
                     FVEADGLFISRQGKGKRAKEEKILAVHEGWKRNGSQLEFVNRRHYLHEGEGDVWERFE
                     EWLMKEYAYDPCRDLLIINGDAASWITACREYFGKRACFQLDRFHVARELRQCLSGHP
                     RWREVRKKLAKQDEEGLLVELNSAVGTLEDEAKEKQMAAMIRRIESMPGCIRDYREWL
                     SEQGVETTGMRPMGHAESVMSRFAHRVKSRRSWKDQGLRAFLRAMAARIDGIWRRNGQ
                     LVEEEETRTAASASTKPKRIEQAKRKAGRLWADVVRQNLPCLQRSSGTPIHQALSALR
                     DGGWM"
BASE COUNT          431 a          417 c          585 g          369 t
ORIGIN      
        1 gatggaccgc gaggcggtgt gagtaaaata tttgtgggtg acgttccgga atgattcccc
       61 gacgagggtt gcgaactttt gttcgcgacg ctcgtcgggg ccaccaagcg aagcgcggta
      121 gaaaaaagca aatgtcatgc aaaaaggact ctctccctgc tatgatggtg attgtcaaca
      181 taaaccgaaa acaggaggga gagagtccat gaaacatctt accacagaat ggcctttatt
      241 aaaagagctg gaggaacaat tagtcagaac tcttcaaaag gtgttcgctg tcttgttggc
      301 ggcccttttg gaggagattg atcaacaact ggcggaagcg cgggacaagc gccggtatca
      361 gctgaaagac aaacggccga ctacgatcca aacgctgttt ggagaagtga cgtttcgacg
      421 gaactactac tatgaccggc aaacggggaa ctataccttc ttgctggatg ccgaattagg
      481 ctttgatggg gcccgatcga tcagtccttg cctcgaggaa acggcggtcg agttggccgt
      541 agagtgctct tcctaccgca aagcggcccg tacgttggag tcgatcgtgg ggtatgcggt
      601 catgagccac gaggcgattc gccaactggt gctggaggcc cctgtctcgc tgcaccgccc
      661 tgtttcccaa cggcacggcc gggtgctgtt tgtggaggcg gatggactgt ttatttcccg
      721 ccaggggaaa gggaaacggg cgaaagaaga gaaaatcctg gcggttcacg agggatggaa
      781 acgaaacggt tcgcagctcg agttcgtgaa ccggcgccac tacctccatg aaggggaggg
      841 agacgtgtgg gaacggtttg aagagtggct gatgaaggaa tatgcctatg acccgtgccg
      901 ggaccttttg atcatcaacg gcgacgcggc gtcgtggatc acagcctgcc gggagtattt
      961 tgggaagcgg gcgtgctttc agctggatcg atttcatgtg gcgcgagagc tgcgtcagtg
     1021 tctgtccggc catccgcgtt ggcgggaggt gcggaagaag ctggcgaaac aagacgaaga
     1081 agggcttctg gtggagctga acagcgcggt cggcacgttg gaggacgaag cgaaagagaa
     1141 gcagatggct gccatgatcc gtcggatcga gtcgatgccg ggatgcatcc gggactatcg
     1201 ggagtggctg tcggagcaag gggtggagac gaccggcatg cgtccgatgg gccacgccga
     1261 gagcgtgatg agccggtttg cgcatcgggt gaaatcccgc cgcagctgga aagaccaagg
     1321 gcttcgggcg tttctgaggg cgatggcagc ccgaatcgac gggatttggc ggagaaatgg
     1381 gcagttggtg gaggaagaag agacccgaac ggcggcctcg gcctcaacaa agcccaagcg
     1441 gatcgaacag gccaaacgga aggccggacg gttatgggca gatgtggtgc gtcagaatct
     1501 accgtgtctg cagcggtcat ccgggacgcc gatccatcaa gcgttgtcgg cgctccggga
     1561 tggtggttgg atgtaaaaaa atggaatatc gtatcatcgc ctcaagatga gggatgagag
     1621 tccgaaagcg cttacgatat gaattcctga aaatggttcg ctaactagtg attgacaaca
     1681 cccgtagtga accgaaaaaa tgttctccac aaagtcttga ctgactcatt gttcctccag
     1741 ctcttttaat aaaggccatt ctgtggtaag atgtttcatg gactctctcc ctcctgtttt
     1801 cg
//
LOCUS       DATMTI010000676         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_25819, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000676 DATMTI010000000
VERSION     DATMTI010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25819"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VN039_10860"
     CDS             <1..423
                     /locus_tag="VN039_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46500.1"
                     /translation="DFEHARLLPAARTYRAWLERMSSHRHWVMAAAILTIFVEGSVKD
                     RKEILAPPDTKHPEDIESIISLHPLVRYHGVSPSRMDLIRAHQMVEAGHRHDAYDMVV
                     NYTPPTLRQSVLTSVKKSLSLWLNYRDAVAKACGVQIP"
     gene            423..1310
                     /locus_tag="VN039_10865"
     CDS             423..1310
                     /locus_tag="VN039_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWV46501.1"
                     /translation="MARSFLSLCVLFLAVSYYPSAIGADISTEDMALIPSGEFTMGSP
                     EGSDGFPDERPERRVYLSEFFVDRFEVTNQAYAVFVLATGHRSPANANQAATLWTNDR
                     PIPGIEHHPVVNVSWEDAEAYCRWAGKRLPTEAEWEKAARGTDGRRYPWGNTWDFTMA
                     NSASYWAQRTIEFNSGADWDAFWVRGEGARLAKEKGIQGEVLTMPVDSFPQARSPYGL
                     FGMAGNAAEWVQDWYDPNYYKHAPLTDPPGPIRGAIKAMRGGSWLKPAASLRTTDRDW
                     GTMDSRPSGAGFRCAKDAY"
BASE COUNT          324 a          445 c          419 g          318 t
ORIGIN      
        1 gatttcgaac atgcccgtct gttgcctgcg gcccgaacct atcgcgcctg gcttgagcgc
       61 atgtcgagcc accgccattg ggtgatggcc gctgcgatct tgaccatctt cgttgaaggg
      121 agcgtgaagg atcgcaagga aattctcgcg cctcctgaca cgaaacatcc ggaagatatc
      181 gaatccatca tcagccttca tcccttggtc cggtatcatg gggtttcacc aagccgaatg
      241 gacctgattc gagcccacca aatggtcgaa gcaggccatc ggcacgatgc ctacgacatg
      301 gtcgtcaact acacaccacc cacccttcgg caatcggttc tgacatcggt aaaaaaaagc
      361 ttgtctctgt ggctgaacta tcgcgatgcg gtcgccaagg cttgtggtgt ccagattccc
      421 taatggcccg atccttcctc tccttatgtg tgctcttcct tgccgtcagc tattacccgt
      481 cggctatcgg cgctgacatt tccacagaag atatggccct gattccttcg ggagagttta
      541 cgatgggaag ccccgaagga agcgacggct tccctgacga acgtccggaa cgacgtgtct
      601 atctcagtga attcttcgtt gaccgttttg aggtaaccaa ccaggcctat gccgtcttcg
      661 tgctggcaac gggtcatcgc tctccggcga atgcaaacca agccgcaact ctctggacga
      721 acgatcgccc catcccgggc atcgaacatc atcccgtcgt caacgtgagt tgggaggatg
      781 ccgaagccta ctgccgttgg gccggaaaac gccttccgac cgaagcggaa tgggaaaagg
      841 ccgcgcgcgg aaccgacgga cgacggtacc cctggggaaa cacctgggac tttaccatgg
      901 ccaatagcgc cagctattgg gcgcaacgga cgatcgagtt caacagcggg gcggattggg
      961 atgctttctg ggtcagaggc gaaggggctc gcctggccaa ggagaagggg atacagggag
     1021 aagtcctcac catgccggtt gacagttttc cccaagcacg aagtccttat ggtctattcg
     1081 ggatggccgg caatgccgcg gaatgggtcc aagactggta cgatccgaat tattataaac
     1141 atgccccgct cacagacccc ccgggtccga ttcgaggtgc gatcaaagcc atgcgcggcg
     1201 ggtcatggct caaaccggct gccagcctcc gcacaactga tcgcgattgg gggacgatgg
     1261 acagccgtcc aagcggtgcc ggctttcgct gcgcaaaaga cgcctattag caggatgttg
     1321 aaaaagtccg ccagcttcgt tctcgcgtcc ctcagaggct caacgtaccg aagcgtacgc
     1381 ctcgcctctt cactcgctgc ggccttgctg gacggccttt ttgaccatcc tgcgagagat
     1441 cttcatttga gtggaacctc catggttgtg tctagttgcc aggcggcctt ctgaaaagaa
     1501 cttcca
//
LOCUS       DATMTI010000677         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_449980, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000677 DATMTI010000000
VERSION     DATMTI010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449980"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1828)
                     /locus_tag="VN039_10870"
     rRNA            complement(<1..1828)
                     /locus_tag="VN039_10870"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(2076..>2245)
                     /locus_tag="VN039_10875"
     rRNA            complement(2076..>2245)
                     /locus_tag="VN039_10875"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          475 a          799 c          475 g          496 t
ORIGIN      
        1 gtacgggcgg cgcacagctc gctagaggtt tttctcgaca gcataggatc atccacttcg
       61 tccggttgga ctccccatca ggtctcaggc acatgagacg cggatttgcc tacgtctcgc
      121 cctacaccct tggccgtggg caaccatcgc cacggttgga ctgccttcct gcgtcacccc
      181 atcgcttgac tactaccagt tcgggtcgcg cgctcccccg actcggtccg aagacctcgc
      241 caggttcggg cgcttagcat cgctaggttc gtcatggtcg cgtcagagcc ggtacgggaa
      301 tatcaacccg ttgtccatcg actacgcctg tcggcctcgc cttaggtccc gacttaccca
      361 gggcagatca gcttgaccct ggaaccctta gtcatacggc ggaggggttt ctcacccctc
      421 tttcgctact catgcctgca ttctcactcg tatggcgtcc acggctggtt cacaccgccg
      481 cttcgctcgc catacgacgc tcccctaccc atccacgcac ctggacccga ggcgaacctc
      541 gggccgggtt gttgcatgaa tgccatagct tcggtggata acttgagccc cgctacattg
      601 tcggcgcgga atcacttgac cagtgagcta ttacgcactc tttcaagggt ggctgcttct
      661 aagccaacct cctggttgtc tgtgcgactc cacatccttt tccacttagt catcgcttgg
      721 ggaccttagc tgatggtctg ggctgtttcc ctctcgacta cggagcttat cccccgcagt
      781 ctcactgctg cgctctcact tactcccatt cggagtttgg ctaacgtcag taacctggtc
      841 gggcccatcg gctatccagt gctctacctg gagcaagaaa cacacaacgc tgcacctaaa
      901 tgcatttcgg ggagaaccag ctatcacgga gtttgattgg cctttcaccc ctatccacag
      961 gtcatccccc cagttttcaa cctaggtggg ttcggtcctc cacgtcgtct taccgacgct
     1021 tcaacctgcc catggataga tcactccgct tcgggtcttg atcatgctac tcaaacgccc
     1081 agttaggact cgctttcgct acggctcccc cacacgggtt aacctcgcaa cacaacacaa
     1141 actcgcaggc tcattcttca aaaggcacgc catcacccac cacccccacc aaaagcagga
     1201 gcccgggctc tgacggattg taggcacacg gtttcaggta ctatttcact ccccgccagg
     1261 ggtacttttc acctttccct cacggtactc gtccgctatc ggtcaccaag aagtatttag
     1321 gcttgacggg tggtcccgcc agattcacac agaattccac gagttccgtg ctacttggga
     1381 acatgctcac aagacgtgcg gcttacacct acgggactat caccctctac ggcgccactt
     1441 tccaaaggct tcaacttcac cacacacaac tcgcagccgg tcgacagccc agcaacgaac
     1501 acaccccaca acccccacac agcaacccct gccaggtatc acactacgcg ggtttagcct
     1561 cctccgcttt cgctcgccac tactcacgga atcacaattg ttttctcttc catccggtac
     1621 tgagatgttt cacttcccgg agttccctcc acacacccta tttcattcag gcgcgggtaa
     1681 ctggacatga ctccagccag gtttccccat tcggacaccc ccggatcaac gctcggttgc
     1741 caactcccca gggcttatcg caggctccta cgtccttcat cggctcttga tgccaaggca
     1801 tccaccatgc gcccttcaca gcttgtcacc acaaaacaca cgcgacacgc cacaaagaac
     1861 cacaacacac cccagcacaa tcaaccaggg catgcacaac aacaagaaac acaccaaccc
     1921 accccacaca aaagcaggcc aagccgatgc atctcaagat gctcgcgtcc actatccaga
     1981 tctcaaacaa caaccccaca cgcacaaccc ctgccaggta tcacacacgc acggtttagc
     2041 ctcctccgct ttcgctcgcc actactcacg gaatcacact tgttttctct tcctatgggt
     2101 actgagatgt ttcacttccc cacgttccct ccacacaccc tatttcattc aggcgcgggt
     2161 aactggacat gactccagcc aggtttcccc attcggacac ccccggatca acgctcggtt
     2221 gccaactccc cagggcttat cgcag
//
LOCUS       DATMTI010000678         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_662287, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000678 DATMTI010000000
VERSION     DATMTI010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662287"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VN039_10880"
     CDS             <1..855
                     /locus_tag="VN039_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWV46502.1"
                     /translation="IYTTIMAQLIEAYRKIRNTCRFLLSNLYDFDPVTHRVPYEKLPE
                     LDRWALLRLGELTTKVRQAYEDFEFHTIFHALNNFCSVDLSAVYLDILKDRLYTFRKE
                     AHERRSSQTVLYDIVMAMAKLMAPVLSFTAEEIWRILAAQVAGGMETASVHLASFPEI
                     DPAWPDAKLAERWERLLKYRTQVQGVLEESRRGKTIGSSLEAYVHIEANADNFRFLKP
                     YEQDLSALFIVSNVMLTQSPEGGEPLRVTVSKSQAGKCERCWNYREAVGKDAVHPTLC
                     DRCVEAIR"
     gene            852..1403
                     /gene="lspA"
                     /locus_tag="VN039_10885"
     CDS             852..1403
                     /gene="lspA"
                     /locus_tag="VN039_10885"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWV46503.1"
                     /translation="MSASGLRNLALASVTGSIIFLDQLTKQQIMQTMRLHESISVIPN
                     LLSLTYIRNPGAAFGLLAGSSNAFRMVFFGLTSLFALGLLGTILLRMPEEDWMGRVSV
                     AGILGGAIGNLIDRLRFGEVIDFLDVYVENYHWPAFNVADSAITVGVIFLIIHFAFEK
                     RADPPTASETSSVPQDRQHDPMR"
     gene            complement(1480..1740)
                     /locus_tag="VN039_10890"
     CDS             complement(1480..1740)
                     /locus_tag="VN039_10890"
                     /inference="COORDINATES: protein motif:HMM:NF021169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HWV46504.1"
                     /translation="MSSDTIHVYDTWVNGKSGRIHFDVMTTDEATALKLAKEYLVSIG
                     EPNAPVTTKECQFCHSEPLFMFSAEQQKQTKEKGGFIVPMPA"
     gene            complement(1753..2628)
                     /locus_tag="VN039_10895"
     CDS             complement(1753..2628)
                     /locus_tag="VN039_10895"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWV46505.1"
                     /translation="MNEWGPSLAVILGIVEGLTEFLPVSSTGHLILVGHALGFTGDVA
                     ANAEISIQLGAILAVIVFEREKIERLLSGAWQEQKVLRSASANEPSVAWTDCVKVSMR
                     SHPNLWFLLGLGIAFLPAAILGLLAHGWIKSYLFTPQTVATTSILGGIIILIVEATKR
                     TSHAMSLDQVSAVHAFWIGLAQCASLIPGMSRSGSTIIGGLLAGLDRKVATEYSFFLA
                     LPTIIAATVYETWKARGTFTDQDFLALGLGMLISFLVAWAVIAAFLTYVQRHTLRVFA
                     YYRIILGILVILVVR"
     gene            2928..>3605
                     /locus_tag="VN039_10900"
     CDS             2928..>3605
                     /locus_tag="VN039_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46506.1"
                     /translation="MRREWTAIVLVGAVMTTGCVSNKKYQEALAEADNVKMELDKTRQ
                     QKSAMEQQVRTLKEVNAKFGNEAQAARDELQRIEHGRDAERGAIEGRTKELEDKVKAL
                     SAQNKNVRQEYQDVKRHNDTLKSLVARYQKELKDRSNAGGAAQAATATPSQAPAGTTA
                     PPAAASAAMNINKVSANDMILFLGLKQDEADRIVSNRPYRVKGELVAKNVVPKETFDL
                     IKDRISVS"
BASE COUNT          881 a          920 c         1031 g          773 t
ORIGIN      
        1 atctatacca ccatcatggc gcaactcatc gaagcctatc ggaagatccg taacacctgc
       61 cggtttctct tgagtaatct gtacgatttc gatccggtga cgcatcgtgt tccctatgaa
      121 aaactgccgg agttggaccg atgggcattg ttgcgcctcg gggagctgac cacgaaagtg
      181 cggcaagcct atgaagactt tgagtttcac acgatctttc acgcattgaa caacttctgc
      241 tccgtcgatt tgagcgccgt ctatctcgac attctgaaag accgactcta tacgttccgc
      301 aaggaggcac acgagcgtcg cagctcccag accgttctgt acgacattgt tatggctatg
      361 gcgaaactca tggccccggt gttaagtttc acggcggagg agatctggcg gatattagcc
      421 gcgcaggtag ctggggggat ggaaacggcg agtgtgcatc tcgcctcgtt tcctgagatc
      481 gatccagctt ggcccgatgc gaagctggcc gagcggtggg agcggctgct gaaatatcgt
      541 acgcaggtgc aaggggttct ggaagagagc cgccggggga agacgatcgg ttcttctttg
      601 gaagcttatg tgcacatcga agcgaatgcc gataatttcc gatttttgaa gccgtacgaa
      661 caagacctca gcgccctgtt catcgtctcg aacgtgatgt taacgcaaag tccggaaggt
      721 ggagaaccgc ttcgtgtgac ggtgtcgaaa tcacaagcgg ggaagtgcga acgttgctgg
      781 aactatcgag aggcggtcgg caaggacgcc gtccacccaa cactgtgcga tcgatgtgtg
      841 gaggcgatcc gttgagtgcg tcaggccttc gcaatctggc gctcgcgtcg gtgaccggca
      901 gtatcatttt tctggatcag ctgaccaaac agcagatcat gcaaaccatg cggctccatg
      961 aatcgatttc tgtcattccc aatctcctca gtttgaccta tatccgaaat ccgggggcgg
     1021 cattcggtct gctggccgga agcagcaacg cgtttcggat ggtctttttt gggttgacgt
     1081 cgctttttgc ccttgggctg cttgggacga ttctgttacg gatgccggaa gaagattgga
     1141 tgggtcgtgt cagtgttgcg ggaattctcg gcggcgcaat cggcaacctc atcgatcggc
     1201 tgcgttttgg agaagtgatc gactttttgg atgtctatgt cgagaactat cactggcccg
     1261 cattcaatgt cgcagactcc gcaatcaccg tcggagtgat cttcctgatc attcactttg
     1321 cctttgaaaa acgagccgat cctcctactg cttcggaaac ttcttccgtc ccgcaagacc
     1381 ggcagcatga tcccatgcga tgagcctgta ctcttgaatg ggtcgtgttg aacttgcgct
     1441 caacacgcac ccatttatta tccttattat ccctcacggt taagcgggca ttgggacgat
     1501 aaatccgcct ttttctttgg tttgcttctg ttgctccgcc gagaacatga acagcggttc
     1561 gctgtgacag aattgacatt ctttcgttgt caccggcgca ttcggttccc cgatgctgac
     1621 gaggtattcc ttggcgagtt tgagggcggt ggcttcatcc gtcgtcatca catcgaaatg
     1681 aatgcgaccg ctcttcccat tgacccacgt atcatagaca tgaattgtat ccgaagacat
     1741 gaccgactcc tttcatcgga cgaccaatat gaccaagatc ccaaggataa tacgatagta
     1801 cgcaaagacg cgcaaggtgt gacgttggac ataggtcaaa aaggcggcaa taacggccca
     1861 agccaccagg aacgagatga gcataccaag gccgagcgcc aaaaaatcct ggtcggtaaa
     1921 cgtccctcgc gctttccatg tttcgtaaac cgttgccgcg atgatggtcg gcagggcgag
     1981 aaaaaatgaa tattccgtag ccacttttcg atccagcccg gcgagcaggc ctccgatgat
     2041 ggttgaaccg gatcgagaca tgcccggaat cagagaggcg cattgtgcca gtccgatcca
     2101 gaaggcgtga accgccgaca cctgatccag actcatcgca tggctggttc gtttcgtggc
     2161 ttcaacgatg agaatgatga taccgccgag gatcgacgtc gtcgccacgg tttggggggt
     2221 gaataaatac gatttgatcc atccatgagc caacaggccg agtatggccg ctggcaggaa
     2281 ggcgatcccg agtcccagca agaaccataa gttgggatga ctacgcatgg agaccttgac
     2341 gcagtcggtc catgcgacag atggctcgtt tgcggaggcc gagcgcagaa ctttttgctc
     2401 ctgccaggcg ccggacagaa gccgctcgat tttctcccgt tcaaagacga tgacggcaag
     2461 tatcgcaccc aactgaatgg aaatttccgc attggctgcg acgtcaccgg tgaagccgag
     2521 ggcatggccg accaggataa gatgtcctgt ggacgagacg ggcaggaatt cagtgagtcc
     2581 ttcgacgatt cccagtatca ctgcgagcga ggggccccat tcattcatgc gtacaccatg
     2641 ttaggattta agataagtga gggagaggtt tcaaccttca cagccatttc tgatagaatg
     2701 ggggcgatga tcacagagcg acgacgattc gtcaagcacg gatgggttgt caggggtagt
     2761 cgcgtcagaa cgggaccgat tcccacaaaa gcaaataagt tgacaacgcc cggatgttgg
     2821 cttacacaca aggaacagaa tcggacgaac cacggtcgca gccgagtgga cgtgatgttc
     2881 cacatgatac acaggcaaac cgtaggattt gatggaggga ggattgcatg agacgcgagt
     2941 ggacggcgat agtgttggtt ggggcggtga tgaccacggg gtgcgtatcc aacaagaaat
     3001 atcaagaggc gttggccgag gcagacaacg tcaagatgga actggataag acccgccaac
     3061 agaagagcgc catggagcaa caggtccgaa ccctcaaaga agtgaatgcg aagttcggta
     3121 atgaggcaca agcggctcgt gatgaactcc agcgcattga gcacggacgt gatgccgagc
     3181 gaggcgcgat cgaaggacga accaaggaac tcgaagataa agtcaaggca ttatcggcac
     3241 aaaataagaa cgtgcggcag gaataccaag acgtgaaacg ccataacgac acgttgaaat
     3301 cgctggtcgc ccgctaccaa aaggagctca aggatcgctc caatgccggg ggggcggctc
     3361 aagcagcgac cgcgacccca agccaggctc cagctggaac tacggctcct ccggcagcgg
     3421 cttcggcggc gatgaacatc aataaggtct cagcgaacga catgatcctc ttcctcggac
     3481 tgaagcaaga tgaggcggat cgcatcgtga gcaatcgccc ctatcgtgtg aagggagaat
     3541 tggtggcgaa gaacgtggtt ccaaaagaaa ccttcgacct gatcaaggat cgcatctcgg
     3601 tcagt
//
LOCUS       DATMTI010000679         1607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_408136, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000679 DATMTI010000000
VERSION     DATMTI010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408136"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VN039_10905"
     CDS             <1..480
                     /locus_tag="VN039_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWV46507.1"
                     /translation="SRYPVKRSALIPLLYVAQRDQGYVTESAMHEIAHLLRLTPPQVY
                     ETITFYTMFNLKPMGKFHIQVCKSLMCALVGSDTVIEWIKAKLRIAPGESTADGLFSL
                     SVVECLAACGTAPMMQINDDYYEQLTENKLDRILADLRSTGTSPLKSGPFMWPEPAR"
     gene            582..>1607
                     /locus_tag="VN039_10910"
     CDS             582..>1607
                     /locus_tag="VN039_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HWV46508.1"
                     /translation="MPQHELILLKNMMQPGYTGSLADYEKTGGYQALRNALGKIAPTD
                     VTAMVRKSGLRGRGGAGFPTGVKWGFLPKDYQGPRYLCCNADESEPGTFKDRQLMERD
                     PHQVLEGIVLACYAIGAETAYIYIRGEMVLGSKILEQAIGEARVAGYIGKNILGTGIN
                     ADVWVHRGAGAYICGEETALLESLEGKRGLPRVKPPFPATHGLYNKPTVVNNVETLAN
                     LPHIINRGPEWFAAIGSPPKSTGTRVFCVSGHVKRPGNYEVPMGMTVRELVYEHAGGM
                     RSDKPMKAFIPGGASAPFLTPAQLDVKLDFEHVAAAGSMLGSGGVTVMEEGTSMVWAA
                     LRLMEFFY"
BASE COUNT          366 a          431 c          483 g          327 t
ORIGIN      
        1 agccgctacc cggtcaagcg ttccgccttg atcccactgc tctatgtcgc ccagcgtgat
       61 caaggatatg tgaccgagtc ggcaatgcac gagatcgcgc atcttctcag gctgacgcct
      121 ccgcaagtct atgagacgat cactttttac acgatgttca acctgaagcc gatgggcaaa
      181 ttccatatcc aggtatgcaa atccctcatg tgcgctctcg tcggctcgga taccgtcatt
      241 gaatggatta aagcgaaact gagaattgcg ccaggcgaat cgacggcgga cggactgttt
      301 agcctcagtg tggtggaatg tttggccgcg tgcgggaccg ctcccatgat gcagatcaac
      361 gatgactact atgagcagct gaccgagaac aaactagacc gaattttggc ggatctgcga
      421 tcgacaggaa ccagtccgct gaagagtggg ccatttatgt ggccggaacc agcgcgatag
      481 gaacagatgg ctaatggcgt atagcgtatg gccgggatgg aatggaggtt ttctcggtat
      541 cggccatagg ccatcagcca tacgctcttt gagttaagac catgcctcaa cacgaactca
      601 ttcttctgaa gaacatgatg cagcccggat ataccgggtc gttggcggac tatgagaaga
      661 ccggcggcta tcaagccttg cgcaacgccc tcgggaaaat cgccccgacg gatgtcacgg
      721 caatggtccg caagtccgga ttgcgaggcc gaggcggcgc aggcttcccg accggtgtga
      781 aatggggatt tctgcccaaa gactatcaag gtccccgtta tctctgctgc aacgccgacg
      841 agagcgaacc cggtacgttt aaggatcgtc aactcatgga acgggacccc catcaagtat
      901 tggaaggcat cgtgttggcc tgctacgcca tcggggcgga aaccgcctac atctatatcc
      961 gcggggaaat ggtcctgggt tcaaaaattt tggaacaagc catcggcgag gcacgagtcg
     1021 ccgggtatat cggaaagaac attctcggca ccggcatcaa tgcggacgtg tgggtgcatc
     1081 ggggggcggg agcctatatc tgcggcgagg aaacagccct cttggaatcg ctggagggca
     1141 agcgtggact cccccgcgtc aagccgccgt ttccagccac gcatgggctc tacaataagc
     1201 cgaccgtggt caataacgtg gagacgttgg cgaaccttcc ccatatcatc aaccgagggc
     1261 ccgaatggtt tgcggcgatc ggttcgccgc cgaagagcac cggtaccaga gtcttctgtg
     1321 tcagcgggca tgtcaaacga ccgggcaatt atgaagttcc gatggggatg acggtccgag
     1381 aactggtgta cgaacatgcc ggcggcatgc ggtccgacaa accgatgaaa gcctttattc
     1441 ccggtggagc ctcggcgcca tttctgacgc cggcccaact cgacgtgaag ctggattttg
     1501 aacacgttgc ggcggcggga tcgatgttgg gatccggcgg cgtgacggtg atggaagaag
     1561 gcaccagcat ggtctgggct gcgcttcggc tgatggaatt tttctac
//
LOCUS       DATMTI010000680         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_790191, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000680 DATMTI010000000
VERSION     DATMTI010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790191"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VN039_10915"
     CDS             complement(<1..1728)
                     /locus_tag="VN039_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWV46509.1"
                     /translation="MPSKIEEYALIGDTHTAGLVSRDGSIDWLCLPRFDSAACFATLL
                     GGPEHGRWLLGPAGVVHRIHRRYRPGTLVLETEYETIDGSVTVVDCMPIRERHPTVVR
                     MVEGRKGRVRMQMELVVRFDYGLIVPWVRSLNGMLTAMAGPDAVCLRADVDLHREQQA
                     MTAEFTVSAGQRLWFVLASYASHESPPPALDSEKAVETTEQWWRDWSDRCTYRGPWKQ
                     QVVRSLITLKAMTYAPTGGMVAAVTTSLSEGLRGGCNWDYRFCWLRDVSFTLCALLDS
                     GYKDEAKAWREWVERTVAGDPSDIQVLYGVAGERRLPEQELPWLPGHDGSSPVHIGNQ
                     AFQQFQLDLYGEVMEGMHLARERGIEPEPTAWRLQCQLIDRLESIWESPDEGIWEARG
                     ERAQFTYSKVMAWVALDRAVKGIEQFGLEGPLDRWRNLRAKIHETVCRDGYNPERGTF
                     VRQFGSTALDASLLLIPIVGFLPPYDPRVRGTVQAIERELMVDDGLLYRHGTADSPAQ
                     GVFIACSFWLAHAYVLMGRRAEAERLFDRLLRLCNDVGLLSEEYDPERQRQMGNFPQG
                     LSHLALVNAA"
     gene            2076..>2722
                     /locus_tag="VN039_10920"
     CDS             2076..>2722
                     /locus_tag="VN039_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWV46510.1"
                     /translation="MSETVAEILIDRLVDWGVRTIFSFPGDGINGIFEALRTRSQDIQ
                     LIQVRHEEAAAFAACGYAKFSRRLGVCLATSGPGGIHLLNGLYDAKMDGQPVLAITGH
                     TFHDLIGTHYQQDVDLDKLFMDVAAYNERIMGPAHVRNVVDEAIKTAISKRTVAHITI
                     PKDIQDWTSNGSRSKANIPHHSAGLYTESFPLPPQSLLQQAADIINQGSKIAILAG"
BASE COUNT          619 a          846 c          714 g          543 t
ORIGIN      
        1 cgcggcattg accagagcca gatgagacaa gccctgtgga aaatttccca tctggcgctg
       61 tctctccggg tcgtattcct ccgacagtaa gccgacatcg ttacagagcc tgagaagacg
      121 gtcgaataaa cgctcggcct ctgcgcgccg ccccatcagg acgtaggcat gtgccagcca
      181 gaaactgcag gcgatgaaga cgccctgcgc cggtgagtcg gcggtcccgt ggcgatataa
      241 gagtccgtcg tcaaccatca gttcgcgttc gatggcctgt acggttcccc gcacgcgcgg
      301 atcatagggc ggaagaaatc cgacgatcgg gatcaacaga agactggcat ccaaggcagt
      361 ggagccgaac tgccggacga acgtgcctcg ttctggattg tagccgtcac gacacaccgt
      421 ctcatggatt ttggcacgga gattccgcca acgatccagc ggtccttcca acccaaactg
      481 ttcgataccc ttgaccgctc gatccaatgc cacccatgcc atcaccttgg aataggtaaa
      541 ctgcgcccgt tcacctctcg cttcccaaat cccttcgtcc ggagactccc agatcgactc
      601 caaacgatca atcaattggc actgcaggcg ccacgccgtt ggctccggtt cgattcctct
      661 ctcgcgggcc aggtgcatac cttccatgac ctcgccgtac aggtcgagtt gaaactgttg
      721 gaaggcttgg tttccgatgt gcaccgggct cgagccgtca tggccgggta accacgggag
      781 ttcctgctca ggcaagcggc gttcccccgc cactccatac aggacctgaa tatcagatgg
      841 atcaccggca accgttcgct cgacccattc ccgccatgcc ttcgcttcat ccttgtatcc
      901 gctgtccagc aaggcgcata gggtaaagct gacgtcccga agccaacaga agcggtagtc
      961 ccagttacaa ccgccccgca ggccttccga aagagaggtc gtaacggcgg ccaccatccc
     1021 gccggtcggt gcgtaggtca tcgcctttaa tgtgatcaat gaacgaacta cttgttgttt
     1081 ccaagggccg cgatacgtgc accgatccga ccagtctctc caccattgct ccgtcgtctc
     1141 gacggctttc tcagagtcca aggccggtgg cggcgattca tgagacgcat aggatgccaa
     1201 cacgaaccag aggcgctgcc ccgccgaaac ggtaaattcc gcagtcatcg cctgctgttc
     1261 gcgatgaaga tccacatccg cgcgcaaaca caccgcatca ggaccggcca tcgccgtcag
     1321 cattccgtta aggctgcgaa cccaaggaac aatcaagccg taatcgaacc gtacaacaag
     1381 ctccatctgc atccgaacgc ggccttttcg cccttcgacc atgcggacca cggtcggatg
     1441 gcgctcccta atcggcatgc aatccacgac ggtaaccgat ccgtctatgg tttcgtattc
     1501 ggtttccagg accagggtgc cgggtcggta gcgacgatgg attcgatgga cgactcccgc
     1561 cggccccagc aaccagcggc cgtgttccgg ccctcccagc agcgtggcga aacatgcggc
     1621 ggaatcgaag cgaggcaggc acagccagtc gatcgatcca tcacgggaca ccaagcccgc
     1681 cgtatgggtg tcgccgatca atgcatactc ttcaatcttg gacggcatgg atctcctcaa
     1741 tgcacgcggt ctctgcttcg ctctgaatta gcctcgcatt acttcgcata cttcatacca
     1801 aataccaagt aactcgccat gggggagaag aaacgcgatt tacctggtgt cgatattaga
     1861 cggacgctcg ggggtaaaca cgcgaacgcg gaacaacagt atcgacagag tgtctcgaca
     1921 aagacaaagt gtcatgctcg ggcatcaaca atcatgcaga ttgtcggtca tctctccggg
     1981 catttagctt gcagtttctt cacgagacgg catgttatcg actaccgtgc gggcgcctca
     2041 tctatgcaaa agagaataac aacaggagga gttatatgtc ggaaaccgtc gcagagatct
     2101 tgatcgatcg gctggtcgat tggggagtac ggacgatctt cagtttcccc ggagacggga
     2161 tcaacggaat cttcgaagcc ctgcgcaccc gaagccaaga catacagttg atccaggtgc
     2221 gccatgaaga agccgccgct tttgccgcat gcggctatgc gaagttctcc cgtcgtctcg
     2281 gtgtctgctt ggccacgtcg gggcccggcg gcattcatct gttgaacggc ctctacgatg
     2341 ccaagatgga cggccaaccg gtcctggcca ttacgggcca tacctttcac gatctgatcg
     2401 gcacgcacta ccaacaggac gtcgacttgg ataaactttt catggatgtg gcagcttaca
     2461 acgagcgcat tatggggccg gcgcatgtcc gcaacgtcgt cgacgaagcg atcaaaacgg
     2521 cgatctcgaa acgcaccgtc gcccatatca ccattcctaa ggacattcag gattggacga
     2581 gcaatggatc acgctcaaaa gccaacattc ctcatcacag cgccggtctc tataccgaat
     2641 cgttcccctt gccgccgcaa agtcttctcc agcaagccgc cgatatcatt aatcaagggt
     2701 ccaagatagc catcctggcc gg
//
LOCUS       DATMTI010000681         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_747151, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000681 DATMTI010000000
VERSION     DATMTI010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747151"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..>1572
                     /locus_tag="VN039_10925"
     CDS             292..>1572
                     /locus_tag="VN039_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46511.1"
                     /translation="MPAQTIKISGARQHNLKNLHVEIPREKLVVITGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLDKMEKPDVDFIEGLSPAIAIEQRSAGANPRSTIAT
                     TTEIYDYLRVLYSAVGQPHDPVTGRPLTRQTPQQIVDRVLAYEPGSKIVVLAPLIENQ
                     AGEFRDVLEKLKREGFVRARIDGEMIELDRTEPIRLKKGERHTIEAVVDRLIVRDGIR
                     VRLTDSIETALKWGSNRVVVLRQQAGDGVMEKSTNEKANAPILQYPITPAGEWEECRY
                     STDYGNAETGFNLGELTPRHFSFNSHLGACPACHGLGTQLVVDPELMISDQTKTLAEG
                     AITPWRRGTKRMRAYYRHLQDALVKHFRVNENVPFSELPDSFKTAVYFGTNGDPIEID
                     FGGNSDKSRPGGEKKVTKPFEGLVPQMQRLYEQTE"
BASE COUNT          406 a          418 c          423 g          325 t
ORIGIN      
        1 tcatcgcttc attgatttgg aacgatcatt cccttgagat ctggatcgat aaggtaagtg
       61 agattgcgcc agagcagctc ggaaaggccg actacgtcat ctcgcttact gaattcaaaa
      121 cgacggtgat ccgctaagca aacagccaca aggactacca aggttgccac tggtgagttg
      181 caccagactg gagccactgt gtgtgactgt cagctgttga aaaccaatgc gatgcagctg
      241 gagaaaaggg tcatttgtgc ttaactaaca ggttcgacat atctcgccag aatgcctgcg
      301 cagacaatca aaatcagcgg tgctcgccag cacaatctca agaatctcca cgtcgagatt
      361 ccaagggaga agctggtggt catcaccggt ttaagcggct cggggaaatc ctcgctcgct
      421 ttcgacacgc tctatgcgga agggcaacgt cgttatgtcg aaagcctctc cgcgtatgcg
      481 cggcagttcc tcgacaaaat ggaaaagccg gacgtcgatt tcatcgaagg cttgtcgcct
      541 gcaatcgcta tcgaacaacg cagcgccgga gccaacccgc gatcaacgat cgcaacgacc
      601 acggaaatct acgattattt gcgggtcctt tattccgcag tcggtcaacc gcacgatcct
      661 gttaccggac gcccacttac ccgccaaact cctcagcaga tcgtggatcg ggttttagcg
      721 tacgaaccgg gaagtaagat cgttgtactc gcgccgttga ttgagaatca ggccggtgag
      781 ttccgggatg tcctcgaaaa gctcaagcgc gaaggattcg ttcgcgcgcg catcgacggc
      841 gagatgattg aattggatcg cactgagccg atccgtttga agaaaggcga acggcatacg
      901 attgaagcgg tagtcgatcg gctgatcgtt cgtgatggaa ttcgggtgcg actgacagac
      961 tcaatcgaaa cggctctcaa atggggcagc aaccgcgtcg ttgttttgcg gcagcaagct
     1021 ggtgatggag tcatggagaa atcgactaat gagaaagcca atgctccaat actccaatac
     1081 cctattactc cagcaggcga gtgggaagaa tgccgctact cgaccgatta cggcaacgcc
     1141 gaaaccgggt tcaacctggg tgagctgacg ccgagacatt tctcttttaa cagccatctc
     1201 ggcgcttgcc cggcttgcca cggtctcggg acgcaattgg tggttgatcc ggagctgatg
     1261 atctccgacc aaaccaaaac gctggccgaa ggagcgatca caccctggcg tcgcggcaca
     1321 aaacggatgc gagcctacta tcggcatctg caggatgcgc tggtcaaaca cttccgagtg
     1381 aatgaaaacg tgcccttcag tgaattgcct gactcattca aaaccgcggt ttacttcggc
     1441 acaaatggcg atccgattga aatagatttt ggcggcaact ccgataaatc gcgaccaggc
     1501 ggcgaaaaga aagtcaccaa accattcgaa ggcctcgtgc cacaaatgca gcgcttgtac
     1561 gaacagaccg ag
//
LOCUS       DATMTI010000682         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_493243, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000682 DATMTI010000000
VERSION     DATMTI010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493243"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VN039_10930"
     CDS             complement(<1..590)
                     /locus_tag="VN039_10930"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWV46512.1"
                     /translation="MSKKKMDAKAFAAQKRNRRRMLTFMRMVRYGINNFSRNAWLTIA
                     ATAVMTITLLIIFTTIAARNVLADTITTLQSKVDMSIYVKHDIGGEAVTSLESDLAKL
                     PGVKSVDYVSPDEGRKNLAHQNSADVDVLNAINEATLNLPGVFNVKVDDINNTSQLDK
                     FVKTNQTYLDNKDPSHEPSFAGKRRQSIQNIGRALS"
     gene            complement(587..1273)
                     /gene="ftsE"
                     /locus_tag="VN039_10935"
     CDS             complement(587..1273)
                     /gene="ftsE"
                     /locus_tag="VN039_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWV46513.1"
                     /translation="MILLDRVTKIYGKNTSKPAINRVSLHIEPREFVILVGTSGAGKS
                     TLLKLLTREEKPTSGKIVVGGIDYDTLKDKHIPLLRRKIGVVFQDFKLLPQRTVYENV
                     AFALEIAGMTNREIRNTVPKVIELVGLTGKEKQFPHQLSGGERQRVAIARAVVRQPKI
                     LIADEPTGNLDPRHSWDIVRLLEKINKYGTTVLLTTHNVEIVNKLKRRVVTIEHGKIT
                     SDQAEGSYRQ"
     gene            complement(1321..>1507)
                     /locus_tag="VN039_10940"
     CDS             complement(1321..>1507)
                     /locus_tag="VN039_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46514.1"
                     /translation="DGQDAREITATGMKDGKKVGTIHMMVVYGEKNFYYVIVAAHVGD
                     TSFNNAAGKIMSSFKIK"
BASE COUNT          318 a          379 c          380 g          430 t
ORIGIN      
        1 aagctcagtg cccggccgat attctggatt gattggcggc gcttaccggc gaatgacggt
       61 tcatgagacg gatctttgtt atcaagatag gtctggttcg ttttgacgaa cttgtcgagc
      121 tgcgatgtgt tattgatatc atcaaccttg acattgaaga cacccggcag gttcaacgtg
      181 gcttcattta tagcgttcag gacgtcgaca tcggcgctgt tctggtgggc gagattcttg
      241 cgaccttcat cggggctgac ataatcgacg cttttgacac ccggtagttt cgcaagatct
      301 gattccagac tcgtcactgc ctctccgcca atgtcatgct tcacgtagat ggacatgtcg
      361 actttcgact ggagcgtcgt gatcgtatcg gccaggacgt tgcgggcggc gattgtagta
      421 aagataatca gcagcgtgat ggtcatcacg gcagtagcag caatagtcaa ccatgcattt
      481 cgcgagaaat tgttgatacc atatcgaacc atccgcatga atgtgagcat acggcggcgg
      541 ttgcgctttt gagcggcgaa cgccttggca tccatctttt tcttgctcat tgccggtagc
      601 tcccttctgc ctggtcactg gtgatcttac cgtgttcaat tgtgacaacg cgtcgtttca
      661 atttgttgac gatctcgacg ttatgcgtcg tcagcaggac cgtcgtaccg tacttgttga
      721 ttttctcaag cagtcgcacg atgtcccagc tgtggcgcgg gtcaaggtta ccggtcggct
      781 cgtcagcgat caggattttc ggttgtcgga ctactgccct ggcaatcgcg acacgttggc
      841 gttcaccacc tgagagctga tgcggaaatt gtttctcttt accggtcaga ccgactagtt
      901 cgatgacttt cgggaccgta ttacggattt cacggttggt catgccggcg atttcaagcg
      961 cgaacgcgac attctcataa acagtgcgtt gaggcaacag tttgaagtcc tgaaagacaa
     1021 cgcctatctt gcgccggagc aacgggatgt gtttatcttt gagtgtatcg taatcgattc
     1081 cacccaccac gatcttgcca cttgtcggtt tctcttccct tgtgagcagc ttcagaagcg
     1141 tcgacttacc agcaccgctg gtaccgacca aaatcacgaa ttcacgtggt tcgatgtgaa
     1201 gacttacccg attgatagcg ggctttgacg tgttcttacc ataaatcttt gttactcggt
     1261 cgagaaggat cattgcttta tatattatag cataagcact cagccatgct accctgttac
     1321 ctatttgatt ttaaacgatg acatgatctt acctgccgcg ttgttgaagc tggtgtcgcc
     1381 gacatgtgct gccacgatga cataatagaa attcttttca ccgtatacga ccatcatgtg
     1441 gatcgtaccg acttttttgc cgtctttcat gccggtcgcc gtaatctcac gcgcatcctg
     1501 tccgtcg
//
LOCUS       DATMTI010000683         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_279940, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000683 DATMTI010000000
VERSION     DATMTI010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279940"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VN039_10945"
     CDS             <1..403
                     /locus_tag="VN039_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HWV46515.1"
                     /translation="AELDRIRQQDLDLIAEARHEGIDLTEEALKRLPFEVELANQLLK
                     KRTFSWTKFLTELEQAIPSRLVLSSVRLDQAGTMVRLTGTAMSLEDITAFTIGLRDHA
                     TFKDPILAQHRVGPNGLVEFDITLQYWHEGT"
     gene            407..982
                     /locus_tag="VN039_10950"
     CDS             407..982
                     /locus_tag="VN039_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46516.1"
                     /translation="MKDRLLFLLWQHPFAPLLPWVGVALSLLLMLLLVQDLGVKGAQA
                     SRERLENEWAATRQALVYHREARKAKMDLSRVWAVLPAERDFTPLALGISDEAKRAQV
                     TLPALSYKTEPTLVANTSKGLLQGPMTGRYEDLRRFIYGLETAEELLFIEDLELTRAG
                     GARDALLTFNIRIATYLRGNTGESGLPRLAQ"
     gene            985..1581
                     /locus_tag="VN039_10955"
     CDS             985..1581
                     /locus_tag="VN039_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46517.1"
                     /translation="MDAKRKTILAASLIFLWAGLAVWQWRLLQEPVRIALTNVTGHAP
                     VGRQVETGRAGLHVNLDLLASTGLQRQATFTVLRNIFAVPRSDGTFAAEDATAFVSQP
                     SASVKTMVQQEDALELEQYRYLGFLRVGEKRGNNNDIAVLRKDDEVLVLRVGDRVDDH
                     LILKAINSESVTIRNTGTHSDQWIPLSEESSEEPMAQE"
BASE COUNT          394 a          405 c          495 g          400 t
ORIGIN      
        1 agcagaactg gatcgaatac ggcaacagga tctagacttg attgcagagg cccggcatga
       61 aggcattgat ttgactgaag aggcgttgaa gagactgcct ttcgaggtcg agttggctaa
      121 tcagctgctt aaaaaaagga cattttcttg gacaaaattt ctgaccgaat tggaacaagc
      181 gatcccttca cgtcttgtgc tcagcagtgt tcgccttgat caagccggca ctatggttcg
      241 actcacgggg actgccatga gcctcgaaga tattactgct ttcaccatcg gactgcggga
      301 tcacgcaaca tttaaggacc cgatcttggc gcaacatcgt gttgggccga acgggctggt
      361 ggaattcgac atcacattgc aatactggca cgaaggaact tgagcgatga aagatcgcct
      421 gctcttcctc ctctggcagc accccttcgc acccttgctt ccatgggtgg gagtcgctct
      481 gagtcttctt ctcatgctgt tgctcgtcca ggatcttggt gtgaaaggcg ctcaagcgag
      541 ccgagagcgt ttggagaacg aatgggctgc gactcgccag gcgctggtgt accaccgaga
      601 agcaagaaaa gcgaaaatgg atttgagccg agtatgggcg gtgcttcccg ccgagcgcga
      661 ctttactccg ttagcactcg gaatttcaga cgaagcaaag cgtgctcaag ttaccttgcc
      721 ggcattgtcc tataagacgg agcctactct cgtcgcgaat acgagcaaag gattgttgca
      781 ggggccgatg accgggcgat atgaggatct ccgccgattc atctatggtc tcgagacggc
      841 ggaagagttg ctgtttatcg aagatcttga gctgactcgg gccggaggcg cgcgggatgc
      901 gctgttgaca ttcaatatta ggattgcaac gtaccttcgc ggcaatactg gggagtctgg
      961 tctgccaaga ctagcgcagt agccatggat gcaaaaagga agacgattct tgctgcctcg
     1021 cttatctttc tgtgggcagg actggctgtg tggcaatggc ggctgctgca ggagcctgtt
     1081 cgtattgcgc tcacgaatgt tactggtcat gcgcccgtgg ggcgacaggt agagacggga
     1141 agagctggtt tgcacgtaaa tctcgatttg cttgcatcaa cgggtcttca gcgtcaggcg
     1201 actttcacag tgcttcgaaa catttttgcc gtgccccgct ccgacggcac attcgcagcc
     1261 gaggatgcca cagctttcgt gagtcagcct tcagcttcag tcaagaccat ggtgcaacaa
     1321 gaggatgcat tggaattaga gcagtatcga taccttggat ttcttcgcgt gggcgagaag
     1381 cgagggaaca ataatgatat tgcggtactc aggaaagacg atgaggtgct ggtgctcagg
     1441 gtcggtgatc gtgtcgatga ccatctgatc ctcaaggcga tcaattctga aagcgtgact
     1501 atccgaaaca ccggtactca tagcgatcaa tggattccgt tgtcggagga atcatcggaa
     1561 gaaccaatgg ctcaggagtg atggataatg tggagcctct tggcaccgct gaggcggcaa
     1621 gaaatggggt tctcatacct catggtgatg attaccatca ctcttatggg gcttgccatg
     1681 acgatggctg ctcg
//
LOCUS       DATMTI010000684         1900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_747249, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000684 DATMTI010000000
VERSION     DATMTI010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747249"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..495)
                     /locus_tag="VN039_10960"
     CDS             complement(61..495)
                     /locus_tag="VN039_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV46518.1"
                     /translation="MATVSQIANKRPKSIGPKTSIASAARTMRQARVGSLLIKKGKQL
                     VGIVTDTDIVRRAVATGKPLGKLTVEKIMTAPICTIEGSQSVDDAQDMMADLGVRHLG
                     VTKNGDISGIVSVRDLLRHYKRYAQSNISTDENYSEPKIMQD"
     gene            740..1510
                     /locus_tag="VN039_10965"
     CDS             740..1510
                     /locus_tag="VN039_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWV46519.1"
                     /translation="MLRLRDDQWERIREHFPEEHIPDTRPGRKPVPTRAVLEAVLWIL
                     NTGAQWHLLPQCYPNYKTVHRRFQQWCQREVLREVLTELANTLRDEGAIDERESFIDA
                     TFAAAKGGGGAVGLTKRGKGVKILAIVDRHGLPLSVSTHAANHHEVTLVQLSFEFYML
                     EAKPEHLIGDRAYDSDGLDDDLKQNGVNLIAPHRSTRKLKTQDGRHLRRYERRWLVER
                     FFAWLQWKRRLLIRWEYYATNFLGFVQLACITMLLKQF"
     gene            complement(1543..1773)
                     /locus_tag="VN039_10970"
     CDS             complement(1543..1773)
                     /locus_tag="VN039_10970"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HWV46520.1"
                     /translation="MRGCCCWEGIGLWLERSWAAYLTVISTSLLLPFELYEVIDRLSM
                     VRIGALLLNLVIGLYLVGQLKQHTLSSRASDL"
     gene            complement(1664..>1900)
                     /locus_tag="VN039_10975"
     CDS             complement(1664..>1900)
                     /locus_tag="VN039_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46521.1"
                     /translation="LFSRLIEALHLNADSRIIYALVLKVDALQPHNVLVAGLVSLGYA
                     GMLLLGGYWTLAGTLLGRVSDSDLDESVASVRTL"
BASE COUNT          426 a          552 c          511 g          411 t
ORIGIN      
        1 gatgtgccga gtgctgagtt tgcgaacctc catgcaaaca gttcagcacg cagtactgca
       61 tcaatcctgc atgatcttcg gttcagaata attctcatcg gtcgaaatat tcgactgggc
      121 ataccgctta tagtgccgca gcagatcgcg cacggagacg atgccggaga tgtcgccgtt
      181 cttggtcaca ccgagatggc gcactcctag atcggccatc atatcctgag catcgtcaac
      241 cgattgactt ccttcgatcg tgcaaatcgg agccgtcatg atcttctcga cggtgagctt
      301 ccccagcggc ttccccgtcg ccacagctct ccgcacgata tcggtatccg tcacgattcc
      361 aaccaattgc ttgccctttt tgatcaagag cgaccccacg cgcgcctggc gcatcgtcct
      421 tgccgcactg gcgatcgagg ttttcggccc gatggacttt ggacgcttgt tcgcaatctg
      481 actaacggta gccatctcct ccccctttca tattatcgtg tcatattatc gtggttgact
      541 gccgacgttc gaacgccaac gacgtttggt ccggcgtaga gtatcatgaa tgagcggaac
      601 gtgcgaggcc gttcgctcgc ttggcaggat gtccgtcaca aagtttggag tggcatagta
      661 ctacagccct agtggccgga cactagaacc tatctcaaaa ttgttgacag ggtggataca
      721 tgtccgtata cgtgctctca tgttgcggtt acgcgacgat caatgggagc ggattcggga
      781 acattttccc gaggagcaca tcccggacac tcggcccgga cgcaaaccag tccccactcg
      841 ggcggtcttg gaggcggtac tctggattct gaacaccggt gcgcagtggc acctgttgcc
      901 ccagtgctat cccaactaca aaaccgtcca tcgccgattc caacagtggt gtcaacgcga
      961 ggtgctgcgc gaggtcctca ccgagttggc caatacgctg cgcgacgaag gagcaatcga
     1021 cgaacgcgag agtttcatcg atgcgacgtt tgcggcagcc aagggcggcg gtggggccgt
     1081 gggcctcacg aaacgcggca agggcgtgaa aatcctcgcg attgtggatc gccatgggtt
     1141 gccgctctcg gtgagtacgc atgccgccaa tcatcatgaa gtcaccttgg tgcaactcag
     1201 ttttgaattc tacatgctgg aggccaagcc cgagcatctc attggtgacc gtgcctatga
     1261 cagcgatgga cttgatgacg acctcaagca gaacggggtg aacctgattg ccccgcatcg
     1321 ctccacccgg aaactgaaga cccaagatgg tcgccactta cgtcggtatg agcgtcgctg
     1381 gctcgttgag cgcttctttg cgtggctcca gtggaaacga cggctcctca tacgctggga
     1441 gtactacgct acgaattttc tcggctttgt gcagctcgcc tgcatcacca tgctcctcaa
     1501 gcaattttga gataggttct agtcacttga gcacccagaa tgtcagagat cagacgcccg
     1561 tgagctcagg gtatgctgtt tcaattggcc gacgaggtag agcccaatca cgagattcag
     1621 cagcaacgca ccgatgcgaa ccatcgatag ccggtcgatc acctcataga gttcgaacgg
     1681 aagcaacaga ctcgtcgaga tcactgtcag atacgcggcc caggagcgtt ccagccaaag
     1741 tccaataccc tcccagcaac aacatccccg catagcccag actgacaagc cccgccacga
     1801 ggacgttgtg cggttgcaag gcatccactt tcaggaccag cgcataaatg atccgggaat
     1861 cggcattcaa atgcagcgct tcgatcaaac gggagaagag
//
LOCUS       DATMTI010000685         1551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_239400, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000685 DATMTI010000000
VERSION     DATMTI010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239400"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VN039_10980"
     CDS             <1..960
                     /locus_tag="VN039_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWV46522.1"
                     /translation="EMENLLSAAREDATALSPQAYDESSWKPLVIRVVSMALIGLPIW
                     YLLQRREREEELQRLDEGWAARLHARSEELLAVNNALVSEVSKRIDTEQSLEANRRDL
                     RVLASQLLRLQEEERRRISRDLHDDINQRLALLSIDIEMLEQQLSGAPVPTVRTVRTI
                     HNRIAELSENVRRLAYQFHPSILDDLGISIALRRLVDDFQTRTGMSTRFICKDIPKHV
                     AQDVVTCLYRVAQEGLANISRHAKAKNVLVELQRVRDGLQLRVSDDGVGFDVNRYDGR
                     RGSLGLLSMRERVSLVAGTLDIQSTPGEGTRVCAWVPFKQERA"
     gene            957..>1551
                     /locus_tag="VN039_10985"
     CDS             957..>1551
                     /locus_tag="VN039_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV46523.1"
                     /translation="MSKPRVLLADDHTLVLEGFKKLLEEHCQVVGSVEDGRSLLDAAK
                     RLRPDIVVLDISMPQLNGLDAARRLRKIVPHARLIFVTVHADPDYVNQAFKAGASAYL
                     LKRSAGSELSQAIEAVRNGNYYVTSLIAKDLVHSAISETEPGPGGQNRLPMRQREILQ
                     LVAEGLTLKEIASALGLSPKTVEYHKSKLMEQLGLHTT"
BASE COUNT          343 a          416 c          440 g          352 t
ORIGIN      
        1 gagatggaaa acctgctttc tgctgcgagg gaagacgcca ccgccctctc accgcaggct
       61 tacgacgaat cttcgtggaa accgttagtt attcgggtcg tgagcatggc cctgattgga
      121 ctacccatct ggtaccttct acaacgtcgt gaacgtgaag aggagctcca acggctcgat
      181 gaaggttggg ccgcgcgctt acatgcgcgt agcgaagaac ttctggccgt caataacgcg
      241 ctcgtcagcg aggtgtcaaa acgaatcgat acggagcaat cgcttgaagc caaccgtcgg
      301 gacctccgcg tgcttgcctc gcagcttctt cgcctccagg aggaagagag acggcggatt
      361 tcgcgtgatc tgcatgacga catcaatcaa cgcctggcac tcttgtcgat cgacatcgaa
      421 atgttggagc aacagctttc cggtgccccc gtccctacgg tcagaacggt gcggaccatt
      481 cataaccgca tcgctgaact ttccgagaat gttcgccgct tggcctatca gttccaccca
      541 tccatcctcg atgacttggg tatatctatt gcgctccggc gcctcgtcga cgactttcag
      601 acccgtaccg gtatgagcac ccgcttcata tgtaaagaca tacctaaaca cgtggcacaa
      661 gacgtcgtca cctgcttgta ccgtgtggca caagaaggct tagcgaacat ttcccgccat
      721 gccaaggcaa agaatgtcct cgttgagctc cagcgagtgc gagacggtct tcagcttagg
      781 gtaagcgatg atggagtggg atttgacgtg aatcggtacg atggccggcg aggaagcctc
      841 ggtttgttga gcatgaggga acgcgtttcc ctggttgccg gaaccctgga tatacaatcg
      901 acaccgggcg aggggacacg tgtctgtgct tgggtgccgt tcaaacagga gcgtgcatga
      961 gcaagcctcg cgtactcttg gcggatgatc atacgctggt gctggaagga ttcaaaaagc
     1021 tgttggaaga acattgtcaa gtagtgggtt ccgtggagga cgggcggtct ttgcttgatg
     1081 cggcgaaacg attgcggccg gacatcgtgg tcttggatat ttcgatgccg caactgaacg
     1141 gccttgatgc ggcgcgtcgt ttacgaaaga tcgtccctca tgctcgattg atttttgtca
     1201 cagtgcatgc cgatccggac tacgtcaatc aagccttcaa ggccggcgca tcggcctatc
     1261 ttttgaagcg atcggccggc tccgagctgt ctcaggctat tgaggcggtg aggaacggca
     1321 attattacgt cacttcgttg atcgcgaagg atctcgttca ctccgcgatc tctgaaaccg
     1381 aacctggtcc tggtggccag aaccgcttgc cgatgcgcca gcgagagatc ctccaattag
     1441 tagccgaagg actgaccctt aaagaaatcg cttccgcact tggtctctct ccaaagacgg
     1501 tcgagtatca taagtcgaag ttgatggaac agcttggtct gcatacgacg g
//
LOCUS       DATMTI010000686         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_408377, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000686 DATMTI010000000
VERSION     DATMTI010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408377"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VN039_10990"
     CDS             complement(<1..369)
                     /locus_tag="VN039_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46524.1"
                     /translation="MMTTHSSESEFGDLHRRLFILRVGLLLVVVLLGLRLWHLQIREG
                     PYYRDLSENNRTRLVLLEPARGLIYDRHGVLLANNVPSFSLYVTLEDVKDREVLIQQL
                     TDLLGLDPTLVRKKMTVKGSK"
     gene            complement(379..879)
                     /locus_tag="VN039_10995"
     CDS             complement(379..879)
                     /locus_tag="VN039_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46525.1"
                     /translation="MKALIYFAVIVGLVPIQSVLLPHISMWGVKPDLSFVAVCLVGLI
                     AGELDGLLVGLALGWAMSLFSAQDLISGAVLKGTTGFVAGLAGRQVVYLSPIVLMAGL
                     LVVSCLAGLITPFAIKLSMQQDWWWAIWTVVLPQACLDAVIGGLIYWLMWSRLNIEQL
                     MSESRM"
     gene            complement(876..1748)
                     /gene="mreC"
                     /locus_tag="VN039_11000"
     CDS             complement(876..1748)
                     /gene="mreC"
                     /locus_tag="VN039_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWV46526.1"
                     /translation="MRMVNLRAPYSARRVALVLAVILVLGLLLLPGQLQSVFQQMGSP
                     LGWIISWPLQVVAGIHDRIADVWGRYVALQEVEEENQQLKRELDLLKEQNGQLREASV
                     ATERLSALLEFKKQVLPTSVAAQVIGRDTGNWYKTIILNKGSSDGLQSDQGVITPAGV
                     VGRVVKTTASTAVVLLVTDPNNAIAGLIQRTRDEGIVEGTTQGQARLKYIPLLSNARP
                     GDHVVTSGLVGGFPRGIPIGTIIRIDKEEEALFQSAELSPEVDPNRVEEVLVIQSSSI
                     PTEGERLGVPKPKS"
     gene            complement(1763..2794)
                     /locus_tag="VN039_11005"
     CDS             complement(1763..2794)
                     /locus_tag="VN039_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWV46527.1"
                     /translation="MFGWFSDDLAIDLGTATTLVYVHGKGIVLNEPSVVAVEKKSEKV
                     LAVGTDAKKMLGRTPGNIVAVRPMKEGVIADFEMAEQMLKHFIRKAHNRSAFVRPRII
                     IGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGSGLPITEPSGNMVVDVGGG
                     TTDIAVISLGGIVYSESVKVAGDRMDEAIMNYIKKKYNLLIGEHMAERIKFEIGSAYP
                     FEERKTMMIKGRDLISGIPRTLVIDDAEVREALQEPIGTIVNAIKIALENTPPELAGD
                     IIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELDLLRKVS
                     VMSQANNLR"
BASE COUNT          644 a          894 c          752 g          641 t
ORIGIN      
        1 cttgctgcct tttaccgtca tttttttccg aacgagggtc ggatcgaggc cgagcaaatc
       61 agtcagctgc tgaatcaaga cttcgcgatc tttcacatct tccagcgtga catagaggct
      121 gaagctcggt acgttattcg ctagaaggac accgtgtcgg tcatagatca agccacgtgc
      181 cggctcgagc aacaccaatc tggtacgatt gttttccgac aagtctctat aatatggacc
      241 ctcgcgaatt tgcagatgcc agagacgcag accaagcagc accaccacca gcaggagtcc
      301 tacacgaaga atgaagagcc gtcgatgaag gtcgccgaat tctgattccg aagaatgcgt
      361 cgtcatcata atgaacgcct acattcggga ctcggacatc aactgctcga tattcaaacg
      421 actccacata agccagtaga tcagtcctcc aatcaccgca tcaaggcagg cttgtggcag
      481 caccacggtc cagatggccc accaccaatc ctgttgcata ctgagcttga tggcgaacgg
      541 agtgattagc ccagccaagc aggaaacgac cagcaatccc gccatcagga cgatagggct
      601 cagataaacc acctgtcggc ccgcgaggcc tgctacgaat cccgtggttc cctttaagac
      661 tgcgccggaa atcaggtctt gcgcggagaa cagactcatc gcccatccga gcgcgaggcc
      721 gaccaacaag ccatcaagct ctcctgctat gaggccgacc aagcacactg ccacaaaact
      781 gagatccggc ttcacacccc acatgctgat gtgcggcagg agaacggact gaatcggcac
      841 aagaccgacg atcacggcga aatagatgag ggctttcatg acttgggctt cggaacgcca
      901 agacgctccc cttcggtcgg aatagaggaa gactgaataa ccagcacttc ctcgacccga
      961 ttcggatcga cctccgggga aagttccgca gattggaaca gcgcttcctc ttccttatcg
     1021 attcttatga tcgtgccgat cggtataccg cgggggaacc cccccaccaa tcctgacgtg
     1081 acgacgtgat cgcccggccg cgcgttggac aacagcggaa tgtacttgag cctcgcctgt
     1141 ccctgcgtcg tcccttcgac tattccctca tctctcgtgc gctggattaa tccggcaatc
     1201 gcgttattgg gatccgtcac gagcaggacg acggcggtgg aggccgtggt cttgacgacg
     1261 cgaccgacca cacctgccgg agtgatcact ccctggtccg attgaagtcc gtcggacgaa
     1321 cctttgttca aaatgatcgt cttgtaccaa ttgccggtat cgcgcccgat cacttgggcg
     1381 gcgaccgatg taggaaggac ttgcttctta aactccagca acgctgagag ccgctccgtc
     1441 gccaccgagg cttctcgcag ctgcccgttt tgttctttga gaagatccag ttctctcttc
     1501 aactgttgat tttcttcttc aacctcttgg agggccacgt atcgtcccca gacatcggcg
     1561 atccgatcat gtatcccggc cacgacctga agcggccaac tgataatcca tcctaagggg
     1621 ctgcccatct gctgaaatac gctttggagt tggccgggta gaagcagtaa gccgagcacg
     1681 agaatgacgg caagaacgag ggcaacacgc ctagcgctgt acggtgcgcg taaattgacc
     1741 atccgcatga ggggatggaa ccttaccgga ggttgttcgc ttgagacatg accgatacct
     1801 ttcggaggag atccagttca tccaggatct tgccgacccc cagcaccacg gaggtcagcg
     1861 gatcgtccac cgtaatgatc ggtaagttcg tttcttctcg gaatcgtgtg tccatccctt
     1921 tcaggaggga acctccccct gtcaacacga tcccgcggtc gataatatcc ccagccagtt
     1981 cgggcggggt gttttccaat gcgatcttga tcgcgttcac gatcgttccg atgggctctt
     2041 gcaacgcttc acgaacctca gcgtcatcga tcaccaacgt gcgcggaatg cccgaaatca
     2101 agtcccgccc cttgatcatc atggttttcc gctcctcgaa cgggtaggcg gacccgattt
     2161 caaacttgat ccgctccgcc atatgttcgc cgatgagcag attgtacttt ttcttgatgt
     2221 aattcatgat cgcttcgtcc atgcgatcac cggcaacctt gacggactcg ctgtacacga
     2281 tgccgcccag tgagatgacg gcgatgtccg tcgtgccgcc tcccacatcg acaaccatgt
     2341 taccggacgg ctccgtgatc ggcaatccgg accctatggc tgcggcgacg ggttcttcga
     2401 tcagatagac ttctcgcgct ccggccaatt ctgccgaatc gcgtacggcg cgttgctcca
     2461 cctgcgtgat gcgggaaggc acgccgatga tgatgcgcgg ccgcacaaac gcgctgcgat
     2521 tgtgggcctt ccggatgaaa tgtttcagca tttgctcggc catctcgaag tcggcgatca
     2581 caccttcctt catcggccga actgcgacaa tattcccggg cgtgcggccg agcattttct
     2641 tcgcgtcggt tccgacagcc agcacttttt cacttttctt ttcaaccgcg acgacggagg
     2701 gctcatttag aacgatcccc ttcccgtgaa catacacgag cgtggtcgca gtacctaagt
     2761 caatcgccag gtcatcggag aaccacccaa acatgtttcc cgggaacccc acggtgtgtc
     2821 tcccttcatc ttggccgagc gatccggcgc ccagcctgtg tgcttcgtgt acgacttacc
     2881 taatccggga ccgccagttg accggcgtct aacacgtgag tccttgctac t
//
LOCUS       DATMTI010000687         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_450306, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000687 DATMTI010000000
VERSION     DATMTI010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450306"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..642)
                     /locus_tag="VN039_11010"
     CDS             complement(127..642)
                     /locus_tag="VN039_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWV46528.1"
                     /translation="MDVRESMTGTPSPVSLLRFLIVVVGGRYLALNAESICGLLTLEE
                     TGNVENPTVHGMVYGTLNLADRLSLPHDAGGANTHVVLLSEGETRGSIRVPMVQGLLE
                     LQPSQVLPLPLHFCGAERQWYRGVILFAKSIAVVLNTTWVLDAQVSDIESGGAKEALL
                     CLRRPQNFCER"
     gene            complement(713..>1843)
                     /locus_tag="VN039_11015"
     CDS             complement(713..>1843)
                     /locus_tag="VN039_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46529.1"
                     /translation="IRAKAVKIGLVQSHRIHTMSDADLLQLIFMPGFSTADRIGDQAG
                     RGVGLDVVKRVIEGMNGHIDVESEPGVGTKFTLNLPLTLLIATALFVRVGTAKYAIPL
                     LSIQEVTMPTLSAVREEGDRKLLQIDEQVIELQSLHYILRREPGNVDWTMPVVIVRTA
                     GGPIGLSVDELLGRQEIVIKPLGPLKLLEHSFFGGATIDQEGRVVLVIDPSRLPSSGT
                     KESAARSPFPKTRPLPEPSLQQEEMLSDKPQEARLLLIDDSLSIRKFVGRMLESAGYP
                     FDTAVDGEDGLRKASAISYRMILTDLEMPKLNGFEVIQALRSRPETRQTPVVVMTTRA
                     RDKHRQMAMSLGANSYIPKPVEEQMLLHEVERWIGKAPALRK"
BASE COUNT          417 a          529 c          448 g          449 t
ORIGIN      
        1 cgaaataccc gatacctctg atgtctgcac tgccaatcgc ctcctcccca ccgaaaacac
       61 caatagctgc cagggtaggc gtacggcgtc ccgatgatgc tggtggccga tcctcagcat
      121 atccggctat cgttcacaga aattttgtgg tcgtcgcaag cagaggagtg cttcctttgc
      181 cccaccgctt tcgatgtccg acacctgcgc atcaagaacc caggttgtat tgagcactac
      241 ggcaatgctt tttgcgaata ggattacgcc ccggtaccat tgtcgttccg ctccgcagaa
      301 atgcaaggga agcggtagga cttgagacgg ttggagttca agaagtccct gcaccatggg
      361 gactcgaatg ctcccacgcg tttccccttc agagagcagc acgacatgcg tgttcgctcc
      421 acccgcgtca tgcggtaagc tcaatcgatc ggccaaattg agggtgccat acaccatgcc
      481 gtgaactgtc gggttttcaa catttccagt ctcttcaaga gtcaacaacc cacatataga
      541 ctcggcattc aatgccagat accgtccgcc cactactacg atgagaaatc gtaatagaga
      601 aactggcgac ggagttcccg tcatcgattc ccgaacgtcc atgccgaggc cttcttgctc
      661 attctctgta acaagccgca tttcgtcaat agatacgagt atttccgcaa ggttacttgc
      721 gcagcgccgg agcctttcca atccatcgtt caacctcatg cagcaacatc tgttcttcta
      781 ccggcttggg aatgtatgaa ttggcaccga ggctcatcgc catctgccga tgtttgtctc
      841 gagccctcgt cgtcatgaca accacaggcg tttgcctggt ttccggacga cttctgaggg
      901 cttgaataac ttcgaatccg ttgagcttcg gcatttcgag atcggtgagg atcatccgat
      961 aacttattgc cgatgctttt cggagcccat cctcgccgtc aaccgccgta tcgaacggat
     1021 atccggccga ctccagcatc ctgccgacga actttcgaat gcttaatgag tcgtctatca
     1081 agagcaggcg cgcctcctgc ggcttgtccg ataacatctc ttcttgctgc aaggatggtt
     1141 ctggaagtgg tctggtcttg ggaaagggag aacgagcagc cgattctttc gtcccactcg
     1201 acggcaaacg gcttggatcg ataacaagaa ctacccgtcc ttcctgatca atggttgctc
     1261 ctccgaaaaa cgaatgttcc aacagtttca gtggtccaag gggtttaatg acaatttcct
     1321 gacggcccaa cagttcgtcg accgataacc cgatcggccc tcccgccgtg cggacgatca
     1381 ccaccggcat ggtccaatcg acgtttcctg gctcccgacg aaggatgtaa tggagagatt
     1441 gtaattcaat gacttgttcg tcgatctgta agagcttacg atcaccttcc tctcgtactg
     1501 cggatagcgt cggcatcgtc acttcctgaa tactcagcaa tggaattgca tattttgcgg
     1561 taccgacccg cacgaacaac gcggtcgcga tcagaagggt aagagggaga ttcaaggtaa
     1621 atttggtgcc gacgccgggt tcagattcta catcgatatg cccgttcata ccttcaatga
     1681 ctcgcttcac cacgtctagt cctacgcctc gacctgcttg atcccctatc ctgtcggccg
     1741 tcgagaaacc gggcatgaaa attaactgaa ggaggtccgc atccgacatc gtgtgtattc
     1801 ggtgagattg tacgagtccg atcttgacgg ctttcgcccg tat
//
LOCUS       DATMTI010000688         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_747314, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000688 DATMTI010000000
VERSION     DATMTI010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747314"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VN039_11020"
     CDS             <1..1306
                     /locus_tag="VN039_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA primase/helicase"
                     /protein_id="HWV46530.1"
                     /translation="ADPIIRWFDEKGISVETIGDFGVYIDDEGAVCFPYGEYGTKRRF
                     GVPTGTRSFRWSKGVDPQLFNLRDSLKPNIFLVEGETDTMRLRQEIGDNQDVGVVGLP
                     GIETWRSDMAEHLHSAQRVWVILDNDQDYGVAGRVDNAWRQIRSDLGPKAKRIYLPKT
                     INDVCEFFEDYDMDALRTIVQRQPKPGESRFKTLDLTLEPPPVHWIVENLICKGDIHL
                     LIGEPGIGKSWLTMGLALAVAGEADNFLGFPVSEHGRVLYLDEENPEDLIYNRMNKLG
                     MSEFAAKNIRYLNNVGVRLDKQADDVIEEALDFEPTLVVLDSLTRFHTEDENHAGSMA
                     GLFHNAIKPLARETGAGVVLIHHANKTDSNSSYKRSRGSGDIPAYVDAGYDVREVGEN
                     SLAVANFKSRRQAQGNTFYVSIVDNSSGGVDLLGGSTQSVPF"
     gene            1306..1509
                     /locus_tag="VN039_11025"
     CDS             1306..1509
                     /locus_tag="VN039_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46531.1"
                     /translation="MTVYPHKHDAIARVYESEDRLWVTNLDLLDAGYAPEDFLVVYLN
                     GMFFELQGYADTPDAWWMERVNA"
BASE COUNT          376 a          363 c          452 g          361 t
ORIGIN      
        1 ggccgatccc atcatccgct ggtttgatga gaaaggtatc agtgttgaaa ccatcggaga
       61 cttcggtgtc tacatcgacg atgaaggcgc tgtgtgtttc ccgtatggtg agtatggtac
      121 taagcggagg ttcggtgtcc cgacgggaac ccgcagcttc cgatggagta agggcgttga
      181 ccctcaattg ttcaaccttc gtgatagtct aaagcccaac atcttcttgg tagagggcga
      241 gacagatacg atgcgactta gacaggagat cggagacaat caggatgttg gtgttgtagg
      301 acttccgggc atcgaaacct ggcgctctga tatggcggag catcttcact cggcacaacg
      361 tgtgtgggtt atcctggaca atgaccagga ctacggcgtt gcaggccggg tggataacgc
      421 ctggcgtcag attagatcgg atctcggccc taaggccaag cgcatttatc tgcccaagac
      481 gatcaacgat gtgtgcgaat tctttgagga ctacgatatg gacgcgctaa ggacgattgt
      541 tcagcgtcaa cctaagccgg gtgaatctcg gtttaagacg ttggacttga ctctggaacc
      601 gcctcctgtc cactggattg tagagaactt gatctgcaag ggcgacattc atctgctgat
      661 cggtgaacct ggtatcggga agtcgtggct tacaatgggc ctggcgcttg ctgtggctgg
      721 tgaggctgac aacttcctag gattcccggt ttccgagcat ggacgggtgt tgtacctcga
      781 cgaggaaaac cctgaggatc taatctacaa ccgcatgaac aagctcggta tgtccgagtt
      841 tgctgcaaag aacattcgtt atctgaacaa tgtaggcgtc cgcctggata aacaggcgga
      901 tgatgtcatt gaggaggcgt tggattttga accgactctt gttgtcctgg acagtctcac
      961 ccggtttcac actgaggatg agaaccatgc agggtcaatg gcgggtctgt tccataacgc
     1021 catcaagccg ctcgcacgcg aaactggtgc gggggtcgtc ctcatccacc atgcaaacaa
     1081 gaccgactcc aactcaagct acaagcgatc aagaggatcg ggagatattc cggcatatgt
     1141 tgacgcgggc tatgacgtta gggaagtcgg agagaatagt ctcgctgtgg cgaactttaa
     1201 gtcaagacga caagcccagg gaaacacgtt ctatgtaagc attgtggata acagtagtgg
     1261 aggggtagac ctgctcggag gatcgacgca gagtgtgcca ttctaatgac ggtatacccc
     1321 cacaagcatg acgcaatagc tcgtgtctac gagagcgaag atcgtctatg ggtgaccaac
     1381 ctagacctgc ttgatgcagg atatgcaccc gaggactttc ttgtcgtata tctcaatggc
     1441 atgttctttg agctacaggg ttatgcagat acgcctgacg cttggtggat ggagcgcgtc
     1501 aatgcctaag cggagacgta gctatccaaa gaagcacagg cagttgctcg ac
//
LOCUS       DATMTI010000689         1694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_67829, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000689 DATMTI010000000
VERSION     DATMTI010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67829"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..361)
                     /locus_tag="VN039_11030"
     CDS             complement(8..361)
                     /locus_tag="VN039_11030"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV46532.1"
                     /translation="MTPPKKDDQKALQPRGRRRVQVDYPALFTGDEGSGHGTVTNLTI
                     AGCEIESHVQFPIGAGLSLRVQAPEARPPIIIALAIVRWKNGDRFGLEFVRFEGRTKE
                     QLGDMLNQREGPVED"
     gene            complement(382..>1694)
                     /gene="purF"
                     /locus_tag="VN039_11035"
     CDS             complement(382..>1694)
                     /gene="purF"
                     /locus_tag="VN039_11035"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWV46533.1"
                     /translation="HRGQEASGIVAGDGDQFFIQKGMGLVADIFHKSVLEKLPGHMAI
                     GHNRYSTTGGHDLKNVQPLTVNFALGNLALAHNGNLINAQMLRHELEAYGAIFQSTSD
                     SEVIIHLIAHSRAGSFLARVVDALSQVRGAFSVVLMTDNGLIAARDPYGLRPLCIGRL
                     RSSWIVASETCAFDLLDAEYVREVEPGELIVISDQGLDSHHPFPKKNPAMCVFEYVYF
                     ARPDSRIFGANAVYATRKALGRQLAQESWVPADVVIPVPDSGVPAALGYSEGAGIRFE
                     TGLIRNHYVGRTFIEPEQSIRHFGVKVKLNAVPEVLNGKRVVVVDDSLVRGTTSRKIV
                     KMIRQAGAKEVHMRISSPPIVSPCFYGIDTPTKKELIASDHSTEEIRKYITADSLAYL
                     SLDGMLKSTPRTPDQYCTACFTERYPIPFTRAEELQLGLFESAR"
BASE COUNT          378 a          477 c          462 g          377 t
ORIGIN      
        1 ggggaaatta atcttcgaca gggccttcac gctgattcag catatccccg agctgttcct
       61 tggttctacc ctcaaatcga acgaactcga gtccaaatcg atcaccattc ttccaacgga
      121 cgatggcgag ggcgatgata atcggaggtc gagcctccgg agcttgtaca cggagactca
      181 gaccggctcc gatggggaac tggacatggc tctcgatttc acatccggca atcgtgagat
      241 tggtcaccgt tccatggcct gatccttcat ctcccgtgaa taatgcgggg taatcgacct
      301 gcactcggcg tctaccccga ggctgcagcg ctttctggtc gtctttcttt ggaggcgtca
      361 tggcttccgt gcagaacgag ttcagcgtgc tgattcgaac agacctaact gaagctcttc
      421 tgcacgggta aatgggatag gatagcgctc ggtaaaacag gccgtacagt actggtcggg
      481 cgtccttggg gtggatttca acatgccgtc aaggctgaga tacgccaaac tgtcggctgt
      541 gatgtacttg cggatctctt ccgtcgagtg atcggaggca atcagctctt ttttcgtcgg
      601 tgtgtcgatt ccgtaaaaac agggcgagac gattggaggg gaactaatgc gcatgtggac
      661 ttctttcgcc cctgcttgtc ggatcatctt gacgatctta cgacttgtcg taccacgaac
      721 caacgaatca tcaacgacga ccacccgttt cccattcaag acttcgggaa cggcattcag
      781 cttcaccttg actccgaagt gacgaatcga ctgttccggt tcaatgaacg tccgtccgac
      841 atagtggttg cggatcaacc cggtttcaaa ccggattccc gccccctcgg aatagccgag
      901 cgccgccggc acaccggagt ctgggacggg aatgacgaca tccgccggca cccatgattc
      961 ttgggccaac tgccgaccca atgccttacg cgtggcatag acggcgttgg ccccaaagat
     1021 gcggctgtcc ggcctcgcaa aatagacgta ctcgaacaca cacatggccg gattcttctt
     1081 gggaaacgga tggtgactgt cgagtccttg atcgctgatg acgatcaact cgcccggctc
     1141 cacctctcga acatactcgg catcgagcaa gtcaaacgca caggtctcgg acgccacaat
     1201 ccagctgctg cgcaggcgcc cgatgcacag cggtctgagc ccatagggat cacgcgcggc
     1261 gataagaccg ttatcggtca tcaacacaac tgagaacgca cctcgaactt ggctcagagc
     1321 atcgacgacc cgcgcgagaa aagagcctgc ccgcgagtgt gcgatgaggt ggataatgac
     1381 ctcgctatcc gatgtggact ggaagatcgc cccgtaggct tcgagttcat gtcggagcat
     1441 ttgggcattg atgagattgc cgttgtgagc caaggccaaa tttccgagcg cgaaattgac
     1501 ggtcaatggc tggacattct tcagatcatg gccaccggtg gtggagtaac gattgtgccc
     1561 aatggccata tggccaggca gtttctccag cacggatttg tgaaaaatgt cggcgacgag
     1621 acccatgcct ttctgtataa agaattggtc tccatctcct gcaacgatcc ctgacgcttc
     1681 ttgcccgcgg tgct
//
LOCUS       DATMTI010000690         6130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_450321, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000690 DATMTI010000000
VERSION     DATMTI010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450321"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..644)
                     /locus_tag="VN039_11040"
     CDS             complement(309..644)
                     /locus_tag="VN039_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46534.1"
                     /translation="MQAVTLLFLAGLLFATGCNRGLGGPLDAASLDPNQDHLKIASFY
                     SRQAMLSRQKAAEQASRALTYEQLFGRDSDWVAGAQMLTRFYEDEAREQDRQASLHLE
                     LAGRRPSNP"
     gene            834..1985
                     /locus_tag="VN039_11045"
     CDS             834..1985
                     /locus_tag="VN039_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46535.1"
                     /translation="MKRIVTALLSVVWLGGCGVLVPYMYDAQILQDRLVVSMPKEQVL
                     KQLGKPNRVVREEGRQIILEYRFYPQGDWTAYLIHCPLFPNCYFPGEAGHPYYVVLQD
                     NQLCLWGTPDVVRPLLGLVCGPTVPLGRKEHVRGGLHISVIPVFMPPPIVPLPQRLAI
                     IPVDGSSDFEISSWLDLTLNFLRTRHPDLVLVEREDLKAVLNEMGIQYGGHVDDETSI
                     HIGRLVGADSLLIYRMIAPEDMPLSAAFELRLFKVESGTTLFRQTVSASQVSPVPVVS
                     GAQRSAKPDSPARRPLLQEAAAYGLAAFTAAFGDNPLGLVPDHGWSGEGVKILDVLQG
                     GPGFRAGLKPGDQIMESDDRPVGSWSDPVTLPAQLTVRHNGVPLRMSVR"
     gene            complement(2171..2611)
                     /gene="cynS"
                     /locus_tag="VN039_11050"
     CDS             complement(2171..2611)
                     /gene="cynS"
                     /locus_tag="VN039_11050"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HWV46536.1"
                     /translation="MEKEAIRSKIKAARREKNLTIANIAKALSKNPTFVAAALNGNHR
                     LTPDEAVKVGKLLDLDNEIVQSLSRFPVRTDLPNMTDPFKYRLLEIVGLYGDSLREMA
                     NEMFGDGIMSAIDFTVDMEKVTGSQGEVRCKITLNGKWLEYKTF"
     gene            complement(2916..4091)
                     /locus_tag="VN039_11055"
     CDS             complement(2916..4091)
                     /locus_tag="VN039_11055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HWV46537.1"
                     /translation="MTQTAQVRLTASRGSESRHLWLYAGHLGSVVGEPIVGDLIDVLT
                     PDGRFYGRGFYSPASKIRVRLLTFQDEPIDETFWLGRIQQAIRLRKRVVAQSNAYRLI
                     YGEGDRLPGLIVDRYDQLLVMQTLSVGMDRRKELLADLLLKELNIAGIYLRNDTKSRL
                     LEGLPLERGFLRGSGATKVEIQEGPAKFLVDVERGQKTGWFCDQRQNRLAAAKFAAGA
                     EALEVFCHTGAFGIHAALAGARSVESLDVSQDTLAMARQHAVMSQVENRCTYRQADAF
                     EEMRHLVKAGRRYDLVILDPPAFARSKQAVRGALAGYKDINLLGFKLLKPDGFLVTCS
                     CSHPVSEADFWNSIRLAARDAKRDIRLIEQRGQSADHPILASMPETRYLKCFIVQVF"
     gene            4264..5925
                     /locus_tag="VN039_11060"
     CDS             4264..5925
                     /locus_tag="VN039_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWV46538.1"
                     /translation="MSDKATIGSAVLNRLHRLGVRHIFGIPGDYVLSLYQLIEASPII
                     HIGTTREDCAGFAADAYARINGIGAVCVTYCVGGLNTVNAIACAYAERSPVVLLTGSP
                     GLSERTRTPYLHHMVRDFGTQREVFERMTVAAVTLDDPLTAEREMDRAFAALLRYRRP
                     IYIEIPRDMVHCQLTGGMKPFCIEDTPSDPAALEEAISEVRIMLASAKRPAMLVGAEV
                     GRFGLQDDLARLVERLNIPIASTLLGKSIIREDHPLYVGVYGGLIGREQVQQFINDSD
                     CLLILGSILSDVEDLDSTSPLLSEGRTIHATADRVAIKHHRYESIKFQDFVQGLVRSP
                     LPSFSHSQRSLPTRSEAVPSAPDLDAPVTLEGLFRYLDTVLTEKTVVIADVGESLFAA
                     ADLHVRHRFEFLSPAYYTSMGFAVPAALGASFADPSLRPIVLVGDGAFQMTGTELASC
                     VRYGQAPIVILLNNRGYSTEREILEGPFNDVHEWQYDKVCDLIGGGRGSRVSTQREFE
                     QNLAAAFADQHQLHLLNVLLSPSDRSPGMVRLAKRLGKKLSTDKP"
BASE COUNT         1251 a         1790 c         1698 g         1391 t
ORIGIN      
        1 gtctcgcacg tctttccatg tattgcacgt gcttcgacgc ttatgggatc cggcatgcgt
       61 gttgacttat taccgttgat gcgcacgcaa tagaaagtgg ccatcggtta caacacctgc
      121 acgatacctg cacaataaaa catttcagat aacgcgtttc cggcatgccg acaaggatcg
      181 gatggtcggc gctttgacct cgttgttcaa tcaaccgaat gtcgcgcttg gcggcgcgac
      241 cggcgggtac ctggcgcatg ggaaatgccg atgcacaggt ccccaggacc aatacaggct
      301 ggtcaaacct aaggattcga gggtcgtctg ccagctagtt ccagatgcag gctggcctgt
      361 cggtcctgtt cgcgcgcctc gtcttcataa aaccgcgtga gcatctgcgc accagccacc
      421 caatccgaat cacgtccgaa gagttgttca taggtcaagg cgcgactggc ttgttccgcc
      481 gccttttggc gggacagcat agcctgtcgg gagtaaaagg aggcgatttt caaatggtct
      541 tgatttgggt cgagtgaggc agcgtcaagc ggccctccca ggccccgatt gcatccggtt
      601 gcgaacagga gaccggctaa gaacaggagc gttacagcct gcatacgatc cagcctttca
      661 tcggcccgta ttatgctatc gcacgcctgc gcgatcaact aaagggtacc gctcatctaa
      721 tcacgcaccc tttcggcatt gatttccctg gcccatgggc tctctagctc agctccacag
      781 tccggcgtac aatcaatcga gtgggatcgt cgtgttggac cggaggaacc gccatgaaac
      841 gaattgtgac cgctcttctg tcggtggtgt ggctgggggg atgcggcgtc ctcgttccat
      901 atatgtacga tgcccagata ttacaagatc ggttggtcgt gtcgatgccg aaggaacaag
      961 tcctcaagca gttgggcaaa ccgaatcgtg tcgttcggga ggaggggcgg cagatcattt
     1021 tggagtatcg gttctatcca cagggcgatt ggaccgccta tctgatccat tgtccgcttt
     1081 ttccgaactg ctactttcct ggcgaagcgg ggcacccgta ttatgtcgtg ttgcaggaca
     1141 atcaattgtg cctgtgggga acgcctgacg tcgtccggcc cctgcttggg ctcgtctgcg
     1201 ggcctacggt tccgctcggt cgaaaagagc atgtccgtgg aggccttcac atttcggtca
     1261 ttccggtatt catgcctcct cccatcgttc ccctgccgca gcgcctggcc atcatccctg
     1321 ttgacgggtc atctgacttc gaaatcagct catggctcga tctgaccctg aatttcctgc
     1381 gcacacgcca tccagacctg gtgctggtgg aacgagagga tctgaaggcg gtgctgaatg
     1441 agatgggcat tcaatacggc ggtcatgtcg atgacgagac gtcgatccat atcgggaggc
     1501 ttgtcggcgc ggacagtttg ctgatctatc ggatgattgc gccggaggac atgccgctgt
     1561 cggcggcgtt cgaactccgc ctcttcaagg ttgaaagcgg caccaccctg ttccgccaaa
     1621 cggtttcggc aagtcaggta tcgccggtac cggtcgtgtc cggggcccag cgctccgcca
     1681 aacccgactc tcccgcccgt cgtcccttgc ttcaagaagc cgccgcctat ggcttggctg
     1741 cattcacggc tgccttcggt gataatccgt taggactggt gccggaccac ggttggtcgg
     1801 gggagggcgt aaaaattctg gacgtccttc aagggggacc gggtttccgt gccggactca
     1861 aaccgggtga tcaaattatg gaatccgacg atcgaccggt cgggagctgg tctgatccgg
     1921 tcacactccc ggcacagttg accgtcaggc ataacggagt ccccctcagg atgtccgtca
     1981 gatgacgggt gaatccgagt gatgtcaggt cgagcatgcg cgggacctat ttgtgaattt
     2041 ttcaatcgtt cagcttcaga gaggcagacg atcttcaccg ccctgtcgat gagatccaac
     2101 acactgagcg aagcgacatc gactctgcct tggccttcaa tccggtaaag gccaggacgc
     2161 gttcatctcg ttagaacgtc ttatactcga gccatttgcc gttcagcgtg attttgcagc
     2221 ggacttcgcc ctggctgccg gtgacctttt ccatgtccac cgtgaaatcg atcgcgctca
     2281 tgatgccgtc gccgaacatc tcgttcgcca tctcacgcaa agaatcgccg tacaagccga
     2341 caatttcgag caaccggtac ttgaacgggt ccgtcatgtt cggcagatcc gtgcgtaccg
     2401 gaaatcggct caaggactgg acgatctcgt tgtccaaatc cagaagcttg ccgaccttca
     2461 ccgcttcgtc cggggtcaac cggtgattgc cgttcaacgc cgccgccacg aacgtggggt
     2521 tcttgcttag ggccttcgcg atgttggcga tcgtgaggtt cttctcgcgt cgcgcggcct
     2581 tgatcttgct acgtatcgct tctttctcca tgttggcctc ccatgaatag gacctctgtc
     2641 aacggccgac acgaggccct gttgaataga tggaccgttg acctcccgtc ggtgaaaaag
     2701 cccgctagcc cacattatta tgacggttcg tgacgaaacc gctaagacgc caagcgagac
     2761 gggcacgcgg agccgcgagg aagggaggca tacttgaaac agtatgtcga ccgactgagc
     2821 ggcgagcctg tccgcttgga tgcggcgaac cggcatccaa gcttgtcgca gcggcgtatc
     2881 ggcggttgca gtagaagctt catgaataat gtgggctaga acacctgcac gatgaaacac
     2941 ttcaggtagc gtgtctccgg catgctggcc aagattggat ggtcggcact ttggcctcgt
     3001 tgttcgatca accgaatgtc gcgcttggcg tcgcgcgccg cgaggcgaat gctgttccag
     3061 aaatcagctt cggataccgg atgagaacaa ctacatgtaa ccagaaaccc gtccggcttc
     3121 aacagcttga atcccaacag attgatgtcc ttgtacccgg ctagcgcgcc gcgtacagcc
     3181 tgcttgctgc gggcaaaggc cggaggatcg aggatcacga gatcataccg tcggccggcc
     3241 ttcaccagat gacgcatttc ttcgaaggca tcggcttggc gataggtaca gcggttttcc
     3301 acctggctca tcaccgcgtg ctgccgggcc atcgccaatg tgtcttgact gacatcgaga
     3361 ctctccacag accgcgctcc agccagggcc gcatgaatgc cgaaggcgcc ggtatggcag
     3421 aatacttcca atgcttcggc tcctgccgca aacttggccg cggccagccg attctgccgt
     3481 tgatcgcaaa accagccggt cttctgcccc cgttcgacat cgacgagaaa ctttgcaggt
     3541 ccttcttgga tttcaacttt tgtcgcacca ctgccgcgaa gaaatccccg ttcaagcggc
     3601 aatccttcga gcaaccggct cttggtatcg ttccgaagat agattcccgc aatgttcaat
     3661 tccttcaaca gcaagtcggc caacagctct ttcctgcgat ccatcccaac ggacaaggtc
     3721 tgcataacca gcaactgatc ataccgatca acgatcaacc ccggcagccg atccccttct
     3781 ccatagatga ggcgataggc atttgactga gcaacgacac gctttcgtaa gcggatcgcc
     3841 tgttgaattc tcccgagcca gaatgtctcg tcgattggtt catcctggaa tgtcagcaga
     3901 cgaactctga ttttcgaggc agggctatag aagccacgtc cgtagaaacg gccgtcggga
     3961 gtaagtacat cgatcaggtc gccgactatc ggttcaccga ccaccgatcc caggtggccg
     4021 gcatagagcc agagatgtcg actctcagac ccacgtgatg cagtgaggcg aacctgtgcg
     4081 gtctgcgtca tacctttttc cgtgccatca tgttgtacct gactctatga gtgagtggtc
     4141 aagtgccgga tgaagaacat cgtacaccgc ccggttgttc ttgacgaagt gtcgtcagta
     4201 tgttttcctg aagtcagaac atttcgccgc gatcgaatag tcatcatcat cgagagtacg
     4261 cacatgagcg acaaagctac gatcgggtca gctgtcttga atcgcctgca tcgtctcggc
     4321 gtccgccata ttttcggcat cccaggcgac tacgtgctgt ccctctacca actgatcgaa
     4381 gcctcgccaa tcatacacat cgggacgacc agggaagatt gcgccgggtt tgcagcggac
     4441 gcctatgctc ggatcaacgg catcggagcg gtctgtgtca cctattgtgt cggcgggctc
     4501 aataccgtca acgccattgc ctgcgcctat gccgagcggt cgccggtggt tctgctgacc
     4561 gggtcaccgg ggctctccga acggactcgc accccctacc tgcatcatat ggtccgagac
     4621 ttcggcacgc aacgcgaagt cttcgaacga atgaccgttg ccgccgtgac gctggacgat
     4681 cctctgaccg cagagcggga gatggatcgg gcctttgccg ccctgctgcg ttatcgccgt
     4741 cctatttata tcgaaatccc cagagacatg gtccactgtc agttgacagg gggcatgaag
     4801 ccattctgca ttgaagatac gccgagtgac ccggccgctt tggaagaggc gatcagtgaa
     4861 gtccgtatca tgctcgcctc ggccaaacga ccggccatgc tcgtcggggc ggaagtgggt
     4921 cggttcgggc ttcaggacga cttggcccgc ctggtggagc gactgaatat ccccattgcg
     4981 tcgaccctgc ttggaaaatc gatcattcgg gaggaccatc cgctctatgt cggcgtgtat
     5041 gggggactga tcgggcgcga gcaagtgcag cagttcatca acgattccga ctgcctgttg
     5101 atcctggggt ccattctgtc ggatgtggaa gatctggact ccacctcgcc cttgctctcg
     5161 gaaggccgca ccatccatgc cacggccgac cgggtcgcca tcaaacacca tcggtatgag
     5221 tcgatcaagt ttcaagattt tgtccaaggc ctcgtgcgat cgccgctccc gtccttctct
     5281 cattcccagc gctcgctccc gacccgaagt gaggcagtac catcggcacc cgatttggat
     5341 gcgccggtga cgttggaagg gctgttccgc tatcttgata cggtgctgac agaaaagacg
     5401 gttgtgattg cggacgtcgg cgaatcgctt tttgcagcgg ctgacctgca cgtgcgtcat
     5461 cggttcgagt ttctgtctcc ggcctattac acgtcgatgg gattcgccgt tcccgctgcc
     5521 ttgggagcct ccttcgccga ccccagcctg cgacctatcg tcttggtggg agacggcgcc
     5581 tttcagatga ccgggacgga gttggcgagc tgcgtccgct atggacaagc cccgattgtg
     5641 attcttctca ataaccgcgg ctattcgacc gagcgagaaa tcttggaagg cccctttaac
     5701 gatgtgcatg aatggcaata tgacaaagtg tgcgacctaa tcgggggtgg acggggttcg
     5761 cgggtcagca cacaaagaga gttcgaacaa aaccttgccg ccgcctttgc agaccaacat
     5821 caactgcatc tcctcaatgt actcctgagc cccagcgatc gatcgcccgg catggtgcgc
     5881 ttggcaaaac gtttgggcaa gaagctttct accgacaaac cataggaaaa accatccact
     5941 cctgcctgct tttccctaca tctccatagg atatttccac tagcctcccg taaaccgtgc
     6001 gtgaagcgat gcggtctagg gtttttgccg ataagcaatg gatggtattc tccctgctct
     6061 cttccactat ttgcatccag gagagtcccc agtatagtct gcactttctt gtctatccgg
     6121 ctttacgcga
//
LOCUS       DATMTI010000691         5212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_577504, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000691 DATMTI010000000
VERSION     DATMTI010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577504"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..860
                     /locus_tag="VN039_11065"
     CDS             495..860
                     /locus_tag="VN039_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46539.1"
                     /translation="MNAVKNNRPTSASHWVRIPDGTKVRHRSEAYEGVIDGLTEIVSG
                     SDRNPDGKTQYRVNVGDSTRLLVSEDHLNILLDNKNLVLLARESELYRRNVTDRLRAV
                     FAEDRFVRIGSKEPTSPGK"
     gene            924..1193
                     /locus_tag="VN039_11070"
     CDS             924..1193
                     /locus_tag="VN039_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46540.1"
                     /translation="MNTSWGTKASHRRKRAIRRTKVRWDLLGLQDPEQTPLTTSIDDI
                     RRQIISAASQGLSPDTISRSHAKAGRPKPGSKASVATRKPGAASQ"
     gene            complement(1414..3186)
                     /locus_tag="VN039_11075"
     CDS             complement(1414..3186)
                     /locus_tag="VN039_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HWV46541.1"
                     /translation="MSPLIGVPDHIAQARKVEGLCPSLPWSSFADFFKSRVYDRALVN
                     RTFLTYCDDDRAVRYAYSYGEFGTVVQSVATFLHDRVGLRPGDRLATILFNHDVTVVL
                     YFAAWISGVTIVPINIEESTDKKRFILEHSEASAVCCWYKLLEEMKDLQRELPALRDV
                     IALNDDGFVEGPKHRVKAGASPFLGTASPSPSLDDEALIVYTSGTTGPPKGVVLTVGN
                     LLLDADAITEWHRFGADARLMCVLPIHHVNGTVVTVITPFYCKGSIVLNRKFKSTTFW
                     RRLHEERVTCVSVVPTILEFLLDANEGIIDYKLDEFGGFICGAGPLLKDTAIRFEDRF
                     GFPIRHGYGLSETTCYSCFLPNDLSRDEHRHWLRDYEFSSIGVPLRHSDMAIMGEDGQ
                     LLPEMARGEICIRGGIVCAGYFKRDDANDAAFQWGWFRSGDEGFYVRDKLGRSFFFIS
                     GRLKELIIRGGVNIAPLEIDEVLRRHPLVRFAMTVPFEHRYYGEEIAAYVVPRDGISP
                     PTEAEVLAHCRQRLPFSKCPKVIRFGQNVPYTSTGKPKRLELKVSLASTLAAYRDYQF
                     KDAEVVQIGEANQRRIDTSGGADQ"
     gene            complement(3252..4430)
                     /locus_tag="VN039_11080"
     CDS             complement(3252..4430)
                     /locus_tag="VN039_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWV46542.1"
                     /translation="MSTVVNESPATEMKDRFAYSPGLEGVIAGESALCLVDEGEAGLL
                     YRGYPIRDLAEHSTFEEVAYLLLFGHLPSQQAFSDFSARLIDQAVLPQLVQVFLGAVP
                     SGAHPMDIVRSGVSLLGVVDSDPADNSHEANVCRSIRLLAQIPLMIATSYRLVTGKPR
                     RQPHDDLTFAENLLYLFTDRREDDQAKAMARVLDASLTLYAEHEFNASTFAARVTAST
                     MTDLYSAITSAIGTLKGTLHGGANEAVAEMFVDIGSCERAEAWVREALAKKRRIMGFG
                     HRVLKKGDSRSPIIQQHVESLSRICGNRRWYEIAMTIERIMEQEKGLHPNLDFYTAVA
                     YLLMEIPLTLYTPLFVCSRVAGWCAHVIEQQDHNRLMRPRALYTGPPRKEYVPLDRRT
                     "
     gene            complement(4443..>5212)
                     /locus_tag="VN039_11085"
     CDS             complement(4443..>5212)
                     /locus_tag="VN039_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HWV46543.1"
                     /translation="IEQSGHEIVYVSGAAISACQGVPDIGLLTLGDMAVEAGRIARAV
                     SIPTIVDADTGYGGPVQVAEAVRIFEEADLAGMQIEDQDMAKKCGHLSGKSLVPVGEM
                     AAKIEAAVRAKRDRNFMIVARTDARGVEGLEAAVQRATTYVGAGADALFPEALESAEE
                     FRTFAHEMAKGGINVPLIANMTEFGKTPLLSVAEFESLGYRGVLFPVTGLRTALQAVD
                     RLLAELKLFGSQKDWLHRMMTRRELYSLLRYTDTHER"
BASE COUNT         1211 a         1480 c         1391 g         1130 t
ORIGIN      
        1 cacgagccgg catgggcgtt cttgtatgca gcgttcttta tcgggatcgt ggtctttgca
       61 cttctgtccg gcggtttcat gggataatcg gtctgtgaac gattgttgca gctagagtat
      121 gatcaagtaa gcgcctaccg ccgaggcgga aatgcctccc ggagccgtcc ataaacggct
      181 ccggtgtttc ctttccaatg atgactcctc ttcttacggt agctctttcg tgttcgcaat
      241 ttaccctatc atgtgataga ctacgctatg cagatttgtt cctcgcgctc ttcccctccg
      301 ttccgttcct gcgcgattga gttgatcagc cccttgcggt gacttcgtcg ggcaaacgct
      361 ctgcttgtca ctcgtcggag gaatccatgg agctgacatc tatgcccgtc ggttttcgtc
      421 agtctctttt tggtttagca ggccgcagaa ggaggacaaa tagagatggg tggtcatgta
      481 gggagtgaat gtccatgaat gccgtcaaga ataatcgacc gacgtctgcg agccattggg
      541 ttcgcatccc ggacgggact aaagtgcgcc atcgatctga agcctatgaa ggggtcattg
      601 acgggttgac cgaaatcgtg tctggttcgg atcgcaatcc ggacgggaaa acacaatatc
      661 gcgtgaacgt gggcgacagt acgcggttac tcgtctcaga ggatcacctt aatattctgc
      721 tcgacaacaa aaatttggtt ctgctcgctc gcgaatccga actgtatcgt cgaaacgtca
      781 ccgaccgact acgggcggta tttgcggaag atcgattcgt aaggattggc agcaaggaac
      841 cgacatcgcc gggcaagtag aggtcgtttg aaggcagatt ctgccgcgca taggtcaaat
      901 gaaaactttg aatcggagaa gcgatgaata catcatgggg aaccaaagcc tcgcaccgac
      961 gaaagcgggc cattcgtaga acgaaggtcc gttgggatct gctcggacta caggacccgg
     1021 aacagacgcc gttgacgacg tcgatagacg atattcgccg ccagatcatc tcagcggcaa
     1081 gccaaggcct gagcccggat acgatctccc gttctcacgc aaaggctgga cgtcccaaac
     1141 caggatccaa ggcgtcggtg gctacccgta agcccggtgc cgcttcgcag taggccttac
     1201 gtaagcacgt cgcacccgaa aggagggcat catcggagga acaggcaaaa ccacaagcgc
     1261 taagcgggac cgtgagaaat cgcgtcagca gaaacaacgc gccaaagaag aacgccgtgt
     1321 acagcggaag gccgccaagc tcaaccggcc gacacaagaa ggaggggaag accctgacct
     1381 tgcgggcttg cgatgggggc cacagcctcc gctctattga tcagcaccgc cagatgtatc
     1441 tattcgtcgt tgattcgcct ctccgatctg taccacctct gcgtccttga attgataatc
     1501 acggtaggct gccagcgtgg acgcaaggga gactttcaac tccagacgct tgggttttcc
     1561 ggtcgaggta tagggtacat tttgtccaaa gcggatgacc ttagggcatt tcgagaacgg
     1621 aagccgttga cggcagtggg cgagtacctc ggcttcggtg ggtggagaga tgccatccct
     1681 tggtactaca taagcggcaa tctcctcgcc atagtagcga tgctcgaacg gaacagtcat
     1741 cgcgaatcgg acaagagggt gcctcctcaa cacctcgtcg atttccaacg gagcaatatt
     1801 gacaccgcct cgaataatca attccttgag gcgacccgaa atgaagaaaa atgatcgccc
     1861 cagcttatcc cggacgtaaa agccttcgtc tccggaacgg aaccaccccc attgaaatgc
     1921 cgcatcattg gcatcgtccc tcttgaagta accggcgcac acaatcccgc cgcggatgca
     1981 tatttctcct ctcgccattt ccggcagcaa ttgtccatcc tcgcccataa tcgccatgtc
     2041 gctatgtcgc agcggaactc cgatcgagga aaactcgtaa tctctaagcc agtgacgatg
     2101 ctcatcacgt gacaagtcat tcggcagaaa acacgagtaa caggtcgttt cggacaagcc
     2161 gtacccatgg cgaatcggga acccgaatcg gtcttcgaac cggattgcgg tatctttgag
     2221 caagggaccg gcgccgcata tgaatccacc gaactcgtcc agcttgtaat cgatgatacc
     2281 ctcgttcgca tcgaggagaa attcaaggat ggtcggcacg acgctcacgc aagtcactcg
     2341 ctcttcgtgt aatctgcgcc agaacgtggt gcttttgaat ttgcgattca ggacaatgct
     2401 tcctttgcag taaaacggag tgatcaccgt aacgaccgtc ccattcacat gatggatcgg
     2461 gagcacgcac atcaggcggg cgtccgcgcc aaaccgatgc cactcggtga ttgcatcggc
     2521 atcaaggagt aaattaccca cggtgaggac cacgcctttg ggtggaccgg tggttccgga
     2581 cgtatagacg atgagcgctt cgtcgtccag actgggactg ggagatgcag tacccaagaa
     2641 gggagacgct cccgccttga ccctatgctt cggcccttcc acgaagccgt catcattcag
     2701 tgcaatcaca tcgcgtaagg ccggaagttc acgttgaagg tccttcattt cttcgagaag
     2761 tttgtaccag caacagacgg ctgaagcctc ggaatgttcc aggataaatc gtttcttatc
     2821 cgtggactct tcgatgttga ttgggacaat cgtgacaccc gatatccatg ccgcaaagta
     2881 cagcactaca gtgacatcat gattgaagag aatcgtcgcc agacggtccc ctggtcttaa
     2941 accgactcga tcatgcagga aagtagcgac cgattggacg acagtcccaa attctccata
     3001 actgtaggca taacggacag cccgatcatc gtcgcaatag gtcaaaaatg tccggttgac
     3061 caaggcgcga tcatagacgc gggatttgaa gaagtccgcg aatgagctcc atggaagtga
     3121 tggacagagt ccttcgactt tcctcgcctg cgcgatgtgg tcaggtacgc caattaatgg
     3181 agacatattc tcttcttgct ggtcccctgt agagggtgcg ggacctcatc aaccgcacct
     3241 gcgtgatgtg gtcaggtacg gcgatcaagg gggacatatt ccttcctcgg cggtcccgtg
     3301 tagagagcac gaggcctcat caagcgatta tgatcctgct gttcgatgac atgggcgcac
     3361 catcctgcaa cgcgcgagca gacgaacagc ggcgtataca gcgtgagcgg gatctccatc
     3421 agaagatagg ccaccgccgt gtagaaatcg agattggggt ggaggccttt ttcctgctcc
     3481 atgatgcgct cgattgtcat cgcaatctca taccatcgac ggttaccgca gatccggctc
     3541 aacgattcga cgtgctgctg aatgatgggt gaacggctgt cacctttctt gagcacgcga
     3601 tggccgaaac ccatgattcg ccgtttcttt gccaatgcct cccgcaccca tgcttccgcc
     3661 cgttcacaac tgccgatatc cacaaacatc tcggccacag cttcattggc tccaccatgg
     3721 agcgtcccct tgagtgtccc aattgccgag gtgatggccg agtacagatc cgtcatcgtc
     3781 gatgccgtga cacgagcggc gaacgtggag gcattaaact cgtgttcggc atacagcgtg
     3841 agagaggcat caaggacacg ggccatggct ttcgcttgat catcctctct tcggtcggtg
     3901 aagagataca agagattctc agcgaaggtc agatcatcgt gcggctgccg gcggggtttg
     3961 ccggttacga gtcgatagga cgttgcaatc atcagcggga tttgcgccag cagccggatc
     4021 gatctgcaaa cgtttgcctc atgcgagttg tcggctggat ccgaatcaac cacgcccaac
     4081 agcgagactc ccgatcggac aatgtccatc ggatgtgcgc cggaaggtac tgcgccgagg
     4141 aatacttgga cgagctgagg aagcacggcc tgatcgatga gtcgcgctga gaagtcactg
     4201 aacgcttgct gactcggcag atggccgaac aacaacagat aggcgacctc ctcaaacgtg
     4261 ctgtgctccg ccaaatcccg gattggatag cccctgtaga ggagaccggc ctctccttca
     4321 tccacaaggc agagagccga ttctccggcg atcactcctt cgagaccagg gctataagcg
     4381 aaacggtctt tcatctctgt cgcgggagac tcgttcacta ccgtgctcat aggtcctcct
     4441 cctcagcgtt catgagtatc ggtgtatcga agcagactgt atagttctcg ccgcgtcatc
     4501 atgcgatgaa gccagtcttt ctgcgacccg aacaacttca gttcagccag cagtctatcg
     4561 acggcttgca aggccgtccg taaccccgta acaggaaaga gcacgccgcg ataccccagg
     4621 ctctcgaatt ccgcgacgct caacagaggc gttttcccga actccgtcat attggcgatc
     4681 aatggaacat tgatccctcc cttcgccatt tcgtgagcaa atgtccggaa ttcctcggcc
     4741 gattccagag cctcaggaaa cagcgcatcc gcgccggcac cgacatatgt cgtcgcccgt
     4801 tgaacggcgg cttccagccc ttccacaccg cgggcatcgg tgcgcgcgac gatcatgaag
     4861 ttccgatctc tttttgcgcg aacagccgcc tcgattttgg ccgccatttc acctacaggc
     4921 accaaacttt tcccggaaag atggccgcac ttcttggcca tatcctgatc ttcgatctgc
     4981 atgccggcaa gatcggcctc ctcaaaaatc ctcaccgctt cagcgacctg aaccggaccg
     5041 ccatatcctg tatctgcatc gacgatggtt ggaattgata ccgcacgggc aatccttccc
     5101 gcctctacgg ccatatcacc gagcgtcaga agaccgatat ccggcacccc ctggcaagcg
     5161 gagatggctg cccctgagac atacacgatc tcgtggccgg attgttcaat tt
//
LOCUS       DATMTI010000692         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_833476, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000692 DATMTI010000000
VERSION     DATMTI010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833476"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            596..829
                     /locus_tag="VN039_11090"
     CDS             596..829
                     /locus_tag="VN039_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46544.1"
                     /translation="MAGRKTIEAKVVEWFETAEVETAWVVLWIVAGKMRERERSGRVA
                     GDTPRHPKAKERVVKKTQAVMATTTQQVANAAD"
     gene            961..1236
                     /locus_tag="VN039_11095"
     CDS             961..1236
                     /locus_tag="VN039_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV46545.1"
                     /translation="MNESPHDNTKDDAGNATLPPPESRVRVVRKLLGLSQWELARTLG
                     VAKRTIARWEAGESEPHEVYVERLEQLVEAGAREHDTMMEVTHGDGT"
     gene            1220..1948
                     /locus_tag="VN039_11100"
     CDS             1220..1948
                     /locus_tag="VN039_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46546.1"
                     /translation="MATGRKGTNPARRTKGVARQNALALFGITTGRMETPEQRESTAL
                     VLRLSQNEKIRALATMMMMAEWKGVAMTTIMEKLQITYGQLAGEYKELQRSQGFIRAA
                     QHLPEIMEQVAIDAKHSATKCETCAGSGEIVVYDHAAIEKARSVVGENTANGFVSDPE
                     MVKLAEGRGKCAICGGRGEVVRLADPEKVKLVFDTFGLTGKGGGVAVNLDLRKLDGAD
                     DLGELAGSLGTILEGSSGGTGTTQ"
     gene            2016..2294
                     /locus_tag="VN039_11105"
     CDS             2016..2294
                     /locus_tag="VN039_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46547.1"
                     /translation="MSLFTFVADVLGVRTELRRIADALERMAPMPDATPVEAIPPEEA
                     VTYVNEEEMTRQELREQLSVLERMRQEHPEWFTGHDLAEDDDLPTRHV"
     gene            2287..>3285
                     /locus_tag="VN039_11110"
     CDS             2287..>3285
                     /locus_tag="VN039_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46548.1"
                     /translation="MYSERVIEQAIARYEAKSGHRLVRLDNGKRDDWRKHLEQRLATT
                     GAQDELERSLTSEEKLFIRNERVMSALSFRYWAHYATIQRDGGGITTFDNPWESTLLF
                     LSFIARIEEEMHDSVARGEMTPYAMAGQPKPAVPGLLVAINKARQLAATTVGRLIAMH
                     RLTTQRDRRALAASVDDDKIQEMYDRDKLVFDNLPWYMRPPVLYDEKAGHIHFDKLNS
                     RVLYQVSSQKSGIGVGRQIDIHHLTELSTWQNPQAVELDFFPAIPRSLGTFGLAESTP
                     YGRGNWWHDWTERVRAGHVDRWRYIYIPWYAEPSKYRSMPPDGWTPSEVALLHAQKV"
BASE COUNT          798 a          804 c         1094 g          589 t
ORIGIN      
        1 gtccctgttt tcatcacagg aatggtgaca ttttattgcc catattgcgg acactgtaat
       61 gtaagacgtg taagatcaac aacttggcat agtacgtgcg ggaacacagc gtgcaggcgc
      121 aaaatgctat tgacagcggt actcgaacgg attccaccgg gagctggtgg tgcaagagcc
      181 ttggccaggc gtcaagcagt cgctgcggga ctagctgcga ttatgaccgt ggaggatgat
      241 gacgatgacc atccaacaga cgacacacga cgacgcgacc cggcgcacga cacgcacgac
      301 aacgctggcg aaggcagcga gccatcgcgc tgatgtccaa cgccgccaat tgtccaaagg
      361 gtaccggcgg ggggatggca tatgtacatg ctcgggagcg tggggaaaat aaaaaatttc
      421 tacgacacgc atgacgatta cgtgagcaat cgaagcgatg tcacacgata gcatgctgag
      481 cccatggcgc gtccaagatt ttcacaactt ttccacaaga catgacgaag gtgacacttg
      541 acatcgcgtg tagtcacgtg tcaccataag tcacgtcacg gggaggtggc gaacgatggc
      601 gggacggaag acgattgagg cgaaggtggt ggagtggttt gagacggcgg aggtcgagac
      661 ggcatgggtc gtgctgtgga tcgttgcggg gaagatgagg gagcgtgaac ggagcgggcg
      721 ggtggcaggc gatacaccac ggcatccgaa ggcgaaagag agagtagtaa agaagacaca
      781 ggcggttatg gcaactacga cacaacaggt ggcaaatgct gcggattaag cctgaggcga
      841 cgcttgtgtg tccgtggtgt tttcacgaat tcgacgtgta cgactcacga cgtcacggtc
      901 gatggtgctc ggacgactgt tactttggtc tgacgccaca ggggacgtat gaggaatatt
      961 atgaacgaat caccacacga caacacgaag gacgacgcgg gcaacgcgac gctacctcca
     1021 cctgagtcac gtgtccgggt cgtgcggaag ttgctgggcc tgagccagtg ggaactggcc
     1081 cggacgttgg gcgtagcgaa gcggacaatt gcgcgttggg aagcggggga gagcgagcca
     1141 cacgaggtat atgtggaacg cttggaacag ctcgtcgagg cgggagcgcg agaacacgac
     1201 acgatgatgg aggtgacgca tggcgacggg acgtaaggga acgaatccgg cacggcggac
     1261 gaagggagtt gcgaggcaaa acgcgctcgc cttattcggg atcacgaccg gacggatgga
     1321 gacgccggag cagcgcgaat cgaccgcgct ggtgcttcga ttgtctcaga atgagaagat
     1381 tcgtgcccta gccacgatga tgatgatggc ggagtggaag ggcgtcgcga tgaccacaat
     1441 catggagaag ttgcagatca cgtacgggca gcttgctggg gagtataagg agttgcaacg
     1501 gtcgcaggga ttcatacgtg cggcccagca tcttccagag atcatggaac aggtggcgat
     1561 tgacgcgaag cacagcgcga cgaagtgcga gacgtgtgcc gggagcggag agatcgtggt
     1621 gtacgaccat gcagcgattg agaaggcacg aagcgtcgtg ggggagaaca cagcgaacgg
     1681 atttgtgagc gatccagaaa tggtgaagct ggccgaggga cggggaaagt gtgcgatctg
     1741 tggcggacgc ggcgaagttg tacgcttggc cgacccggag aaggtgaagc tggtgttcga
     1801 tacgttcggc ttgacgggga agggtggagg ggtggcggtg aatctcgacc tgcggaagct
     1861 ggacggggca gatgatctgg gcgaactggc tggatcgctg gggacgattc tcgaaggatc
     1921 aagtggaggg acggggacga cacaatgatg gtgggacaac tcgtcactgt tatgttgatt
     1981 cttgtcgtga tgttggtgct gggaaggtta ctgccatgag cctgttcacc ttcgtcgcgg
     2041 atgtgctagg cgtgcgcacg gagcttcgcc gcatcgcgga tgcgctggag cggatggcac
     2101 cgatgcctga cgcgacaccg gtcgaagcga tcccgccaga ggaagcagtg acatacgtga
     2161 acgaggaaga gatgacacga caggaactac gtgagcaact gtcagtactc gaaagaatgc
     2221 ggcaggagca tccagaatgg ttcacaggac acgacctagc tgaagacgac gatcttccca
     2281 cccggcatgt atagcgaacg agtcatcgag caggcgattg cacgatatga ggcgaaatcc
     2341 gggcatcggc tcgtccggct cgacaacggg aaacgtgacg actggcggaa gcaccttgaa
     2401 caacgcctcg ccacgaccgg ggcgcaggac gagttggaac gttcattgac atccgaggag
     2461 aaattgttca tccgcaacga gcgggtgatg agtgcattga gcttccgcta ttgggcacat
     2521 tacgccacaa tccagcggga tgggggaggg attacgacgt tcgacaatcc ttgggagagc
     2581 acacttttgt tcctctcttt catcgcccgg atcgaagaag aaatgcacga tagtgtggcg
     2641 cgtggggaga tgacgcctta cgccatggcc gggcagccaa agccagccgt tcccggtctg
     2701 ctggtggcca taaacaaggc tcgtcagctc gctgccacaa cagtcggtcg tctcatcgcc
     2761 atgcacaggc tcacgaccca acgcgaccgc cgcgccctcg ccgcttcggt ggatgacgac
     2821 aagattcagg aaatgtacga ccgcgataag ctggtgttcg acaatctgcc gtggtacatg
     2881 cggccaccag tgctctacga cgagaaggca ggacacattc atttcgacaa attgaatagt
     2941 cgagtacttt atcaggtgtc gagtcagaag tcgggaatcg gcgtaggacg ccagatcgac
     3001 atacaccatc tgaccgagtt gagcacatgg cagaatccac aggcggtcga gcttgatttc
     3061 ttccccgcca tcccacgctc gcttggaacg ttcggcttgg ccgaatcgac accctatggc
     3121 cgtggaaact ggtggcatga ctggacagaa cgcgtccggg cgggacacgt agatcgttgg
     3181 cgttacattt acatcccatg gtacgcggaa ccgagcaagt acagatcaat gccgcccgac
     3241 ggttggacgc caagtgaggt cgccctgctc cacgcgcaga aggtt
//
LOCUS       DATMTI010000693         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_239610, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000693 DATMTI010000000
VERSION     DATMTI010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239610"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..332)
                     /locus_tag="VN039_11115"
     CDS             complement(81..332)
                     /locus_tag="VN039_11115"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HWV46549.1"
                     /translation="MNKRKLLAKALTGSKNLRFTEAVRLAEAFGFRLSRVRGSHHVFV
                     HPILRELVNLQEVSGKAKPYQVRQLLDLVERYNLQLDNE"
     gene            complement(446..820)
                     /locus_tag="VN039_11120"
     CDS             complement(446..820)
                     /locus_tag="VN039_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46550.1"
                     /translation="MKSEHEREAHHRFVLALQHEHLTCTKPGCGGAMAVTDHTLHSAR
                     IKSYEAACGRCHTVEKITGKEEHQPSWDVASITLMAETHLLHEQPICPYDDTPITFSP
                     CPIPAVRLDTASCVTTAVDTRK"
     gene            complement(983..1717)
                     /locus_tag="VN039_11125"
     CDS             complement(983..1717)
                     /locus_tag="VN039_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV46551.1"
                     /translation="MNRHVALITGGAKGIGRGIALDLASRQWNIAFCYRTSEAEAKST
                     AQDIAQRGGQALALRCDVSDPLAAKHMVAQIEKEWRQIDVLINGAGPYHRVNLFDETI
                     EGWNEMFDANLHPIFYLTQAVAPGMKARKAGRIISFGMANADQMEAQADVTAHYIAKA
                     GVLILTRTLAKLLAPYGITVNAISPGFIDSGSAPPQELAAMAKRIPAGYIGTVDDTVA
                     AVRYLLSEEARYVNGANIQISGAWGI"
     gene            complement(1749..2543)
                     /locus_tag="VN039_11130"
     CDS             complement(1749..2543)
                     /locus_tag="VN039_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HWV46552.1"
                     /translation="MKDRRERRPETDNASDPKSPHASRLTPHAALVIFAKAPIPGQVK
                     TRLCPPLTPDEAATLHGSFVLDTLERTKMAASKLKLPIDRYLACAPSVTHVFFKIMEE
                     RQSVKLIDQVGDDLGARMNQAFRTLFAQGYRQVFLMGTDVPTLPFDHFTQALTSLENH
                     DLVLGPAFDGGYYLIGLKRTAPELFADIPWSTERVLRLTQEKAAGIGLKASLIQPWRD
                     VDTLADLEAIIEACAAEAKKPKTERSFSARTTGVLELLAKRLRTRA"
     gene            complement(2577..>3135)
                     /locus_tag="VN039_11135"
     CDS             complement(2577..>3135)
                     /locus_tag="VN039_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HWV46553.1"
                     /translation="AEAFCAEASNARIITAPVGRARQMNEGAKASRGDILLFLHADTR
                     LPAKAILMIEAALTDPAVVGGRFDVTFDSRSVLGTMISTFMNRRSRLSGIATGDQALF
                     VRRRIFEQLGGFADIPLMEDIEFSRRLKRTGQMAALHQTVTTSFRRWEQQGPLRTILS
                     MWALRFMYWAGVNPTHLSRWYKTVR"
BASE COUNT          611 a          867 c          905 g          752 t
ORIGIN      
        1 gtttagatcg ggaatatccg caatatatcc cttgtccgct tcgctataga aaatattgat
       61 gtgataatcc ttcatggccc ctactcattg tcaagttgta ggttatacct ctcgaccaaa
      121 tcaagcaact gtctgacttg ataaggtttg gctttgccgc tgacttcctg aagattgact
      181 aattcacgca ggatcgggtg gacaaatacg tggtggctgc cccttacgcg tgacagtctg
      241 aacccaaagg cctctgccag tcgtaccgct tcggtgaatc gaaggttctt tgagccggtg
      301 agggcttttg cgaggagttt tcgcttattc attcaagcgc atcgatagag acctttcgat
      361 aacaagcagc atgagctagg agattctcag tctccatcct atctcggcgc tggtcctcac
      421 cgcttcgctt ccggcggcgg ccagttcatt tccgtgtgtc gaccgcagta gtaacacagg
      481 aggcggtatc gagccttacg gcggggattg ggcaaggaga aaaggtaatg ggggtatcgt
      541 cgtaggggca gatcggctgt tcgtggagga gatgcgtctc cgccatcaag gtgatggagg
      601 ccacatccca agaaggctga tgttcctcct tgccggtaat cttctcgacg gtgtgacagc
      661 gtccgcaggc ggcttcataa gacttgatgc gggcgctatg gagagtatga tcggtcacgg
      721 ccatcgctcc gccgcagcca ggtttcgtac aagtgagatg ctcatgctga agggccagga
      781 cgaatcgatg gtgagcttcg cgttcgtgtt cagatttcat cggcacccct ctcttttcct
      841 aaactctctt ctctccaaag aacggcatcc aggttggttc cctgcgcgca ttgaccgagc
      901 gcctcccgtt atctagaaat atgattctga ctcttggcgc gcgggcagcg agcactgaaa
      961 ctaacccgat cctctcactc catcatattc cccatgctcc actaatttgg atattggcgc
     1021 cgttcacgta ccgcgcttct tcgctgagca aataacggac tgccgcgacc gtgtcatcaa
     1081 cggttccgat gtacccggcg ggaatgcgtt tggccatggc ggcaagttct tgcgggggcg
     1141 cgctgccgga atcgatgaaa ccgggcgaaa tcgcattcac ggtgatgccg tatggagcca
     1201 agagcttggc caatgtgcgt gtcagaatga gcacgcctgc cttggcgata tagtgggcgg
     1261 tcacgtcggc ttgtgcttcc atctggtcgg cgttggccat gccgaagctg atgatgcgac
     1321 cggctttgcg ggccttcatg ccgggcgcca cggcctgcgt aagatagaaa ataggatgca
     1381 gattggcgtc gaacatctcg ttccagccct cgatggtttc gtcgaacaga ttgactcgat
     1441 gataggggcc ggcgccattg atcaggacat cgatttgtct ccattctttt tcaatctgtg
     1501 cgaccatgtg cttggctgca agtgggtcgg aaacatcgca tcggagtgcc agcgcttgtc
     1561 cgcctcgctg cgcgatatcc tgtgccgtgc tcttggcctc ggcttcgctg gttctgtagc
     1621 agaaggcaat gttccactgc cgcgacgcga ggtcgagagc aatccctcgt ccgatgcctt
     1681 tcgctcctcc ggtgatcagt gcgacgtgcc ggttcacatg gcttcctggt tgatcgatgc
     1741 gtgagtcatt atgctctcgt tcgcagtcgt ttggcgagta attccagcac gcccgtggtt
     1801 ctggctgaaa aagaccgctc ggttttcggt tttttggcct cggcagcgca ggcttcaatg
     1861 atggcttcaa ggtcggccag ggtatcgacg tctcgccatg gttgaatcaa ggaagccttg
     1921 agtccgatcc ccgccgcttt ttcctgtgtg agcctgagca cccgttcggt tgaccaagga
     1981 atgtcggcga agagttccgg cgccgtccgc ttgaggccga ttagataata gcctccatca
     2041 aaagccgggc cgagcacaag atcatggttc tccagcgagg tgagggcctg tgtgaaatga
     2101 tcgaacggca gggtcggcac atccgtcccc atgaggaaga cctgccggta tccttgcgcg
     2161 aacagcgttc gaaaggcctg gttcatgcgc gcacccaggt cgtcgccgac ttgatcgatc
     2221 agcttcacgc tctgccgctc ctccatgatc ttgaagaaga catgtgtgac agacggagca
     2281 caagccagat agcgatcgat cggcagcttc agttttgaag cggccatctt tgttctttcc
     2341 aaagtatcga gcacgaaact gccatggagc gtggccgctt cgtccggagt cagtggtggg
     2401 cagagacgcg tcttcacctg acccgggatc ggtgctttgg cgaagatgac caaggccgcg
     2461 tgaggcgtga ggcgtgaggc gtgaggcgat ttcggatcgg aggcgttgtc ggtttctggg
     2521 cgtctttccc ttctatcttt cataccgctc accccttacg ccttactcct cacgttttat
     2581 ctgactgttt tgtaccatcg gctcagatgc gtagggttga cgcccgccca atacatgaat
     2641 cgcaaagccc acatcgagag gatggtccgc aaggggccct gttgctccca gcgtcggaac
     2701 gatgtcgtga ccgtctgatg cagtgccgcc atctgtccgg tacgcttcag tctccggctg
     2761 aattcaatgt cctccatcag cgggatgtcg gcgaagcccc cgagctgttc aaagatccgg
     2821 cgacgtacga acagggcctg atcgcccgtg gcaatgccgc tcaatcggga acgccggttc
     2881 atgaatgtgc tgatcatggt tcccaagacg gaacgagagt cgaaggtgac atcgaagcgg
     2941 cctcccacca ccgccgggtc ggtgagcgcc gcctcgatca tcagtattgc tttggccggt
     3001 aatcgtgtat cggcatgcaa gaagagcagg atgtcaccgc ggctggcctt cgccccctcg
     3061 ttcatttggc gtgcccgccc tactggagca gttatgatgc gagcgtttga tgcttccgcg
     3121 caaaaggctt cggca
//
LOCUS       DATMTI010000694         8159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_577537, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000694 DATMTI010000000
VERSION     DATMTI010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577537"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /gene="mdh"
                     /locus_tag="VN039_11140"
     CDS             <1..943
                     /gene="mdh"
                     /locus_tag="VN039_11140"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWV46554.1"
                     /translation="MMTRPKITVVGAGNVGGTTAQRLAEKNLYDVVLVDIAQGVPQGK
                     ALDISQAGPVCGYSTQVVGTNDYVETAGSSIAVITSGIPRKPGMSRDELLATNAKIVK
                     SVVMELVSRSPDIILILVTNPLDAMVHVARSVSGLPKSRIIGMAGVLDSARMRTFIAA
                     ELNVEATEVQAMVLGGHGDTMVPLPRYTTVRGRPVSELMSKEKLDAIVKRTREGGAEI
                     VGLLKTGSAFYAPSASAVAMVESIHKDEKQVMPCAVLCEGEYGLKNVVVGVPVKIGRG
                     GAEQILEYELTRDERAALETSAGAVRELCAAVDRLMT"
     gene            1273..3675
                     /locus_tag="VN039_11145"
     CDS             1273..3675
                     /locus_tag="VN039_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HWV46555.1"
                     /translation="MTTETTQTSTDCVQLKIVPILAKELGVGAHQISAVVTLLDGGAT
                     VPFIARYRKEATGNLDDTQLRLLEERLCYLRELEERRVAVLASIEEQGKLTDALRTSI
                     EAATTKQAVEDLYLPFKPKRRTRAQIARAAGLEPLADSLLADPMLHPEQEALKYIRVV
                     PAAEGIEAVNVPDAKTALEGARDILMERFAETADLLAAVRARLWDQGVLTSTVMKDKE
                     TAEEEKFRDYYAYAESIKTVPSHRALALFRGRMLGVLKLELGLGEALEAVVPHPCVAM
                     IAAHAGVEDRGRPADKWLAGVCEWTWRVKLHMTLSTELFVQLRESAEAEAIKIFARNL
                     HELLLAAPAGPKVILGLDPGIRTGCKIAVVDATGKLLETETIYPHQPRNDRQGAVETL
                     ARLVIRNGVELVAIGNGTASRETDKLVGEVIKLIATQKPDHKLAKIVVSEAGASVYSA
                     SAFAAAEFPELDVSLRGAVSIARRVQDPLAELVKIEPKAIGVGQYQHDVDQRALARSL
                     DATVEDCVNAVGVNVNTASMPLLERVSGLNKALARNIVDYRDANGPFPNRAAIRSVPR
                     LGEKTFEQAAGFLRIPDGDNPLDRSAVHPEAYPVVERILTRLGAAIAEVMRRPMVLKE
                     LSPADFIDEKFGLPTVRDIFTELEKPGRDPRPEFKTATFREGVESVSDLQPGMVLEGV
                     VTNVAAFGAFVDIGVHQDGLVHVSALANKFIKDPHEVVKPGQVVRVKVIDVDLKRQRI
                     ALTMRLEDTASRPAGSAQSGGEAIRDSRGRGPSASGQAAARASQPIGAMALALAKARW
                     KS"
     gene            3791..4036
                     /locus_tag="VN039_11150"
     CDS             3791..4036
                     /locus_tag="VN039_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWV46556.1"
                     /translation="MIVTLTSKGQTTIPKPIRDHLGLHAGDQLDFVIEPDGRVTLRPA
                     TIDVMDLAGLLRQKGRKALSLEEMQRGVLKGATRHTR"
     gene            4033..4428
                     /locus_tag="VN039_11155"
     CDS             4033..4428
                     /locus_tag="VN039_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWV46557.1"
                     /translation="MIAIDTNVLVRFLTQDDPNQTRRVQRCFATAKDQDESLFLNHIV
                     LCECVWVLGYSYGFHKAALVDVLDRLLATKQFVIEDKPSVWAAVADYRASQADFSDCL
                     IGIKNRNAGCETTVTLDQGTAKLATFRRL"
     gene            complement(4846..6996)
                     /gene="sthA"
                     /locus_tag="VN039_11160"
     CDS             complement(4846..6996)
                     /gene="sthA"
                     /locus_tag="VN039_11160"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HWV46558.1"
                     /translation="MAHYDLLVIGTGPAGQKAAIQAAKLNKKVGIIERKTVVGGVCIN
                     TGTIPSKSLREAVLYLLGFRQRNIYGDEYRVKKKVVIEDLAFRANHVIKHEVQTVQNQ
                     MARNQVDMFFGSADFLDPHRLRIQGDHEALELTADFIVIAVGTEPARPPDVPFDDKTI
                     IDTDGLLMLKRIPDSIVIVGGGVIGTEYASILAALGIPVTLIDKRPRLLEFVDAEIID
                     ALQQRMKDLGVTLYHDEEVLAIKKEWNKSIHVSLRHNGPIRASTLMYAIGRVGATQSL
                     NLQTVGLQPDSRGRLTVNEHFQTTSPHIYAVGDVIGFPALASTSMQQGRHAACHAFGY
                     PDRTDTSLLPYGIFAIPEISLVGRNEEELRQAEVPYGVGIARYREIARGQLIGDETGM
                     LKLLFHRHTRHLLGVHVIGEGATELIHIGQAVMAFHGKIDYFIDTVFNYPTLAECYKV
                     AALDGMNRMPRPWVPYVSSHEVPLDAGVSTHHSVELPGSLRKGIMSFTNHLRKLGKSI
                     WDAQLSHPFVVALGNGTLPEHKFKYYILQDARFLGDLARVLSAAALKAPDSESALRFN
                     KLAEETIIVERSLHESYGKKWALTAKQMTSVPMAPTNYAYTRHMLAIAASGTAAEITV
                     VALPCAWVYCVVGQHLLRKGPPAKNHPYRDWLMLYASPEFAEVQLWMRKKVDQWAKTA
                     GKEALQRMEDSFIISSRYEWMFWDMAWNEEKWPV"
     gene            complement(7100..7765)
                     /gene="tpx"
                     /locus_tag="VN039_11165"
     CDS             complement(7100..7765)
                     /gene="tpx"
                     /locus_tag="VN039_11165"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HWV46559.1"
                     /translation="MMRTQYAGFIGTLCLSLGLVGCQAMGGSTGAGFSYKNMPVADGS
                     AVAGEGNKVLFKGNPLALAGNGVKVGDMLRDVKVAQNDLSLVNIAETKGAGKVRVISV
                     VPSLDTPVCEQQTHLLSERNKGLDKMVELITVSVDTPFAQKRFAQEAKIANVTFLSDY
                     REGEFGKAHGLFLEGPHILTRAVMVVDKANTIRYLQITPELSQLPDMEEALQFARRLV
                     TES"
     gene            complement(7825..>8159)
                     /locus_tag="VN039_11170"
     CDS             complement(7825..>8159)
                     /locus_tag="VN039_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV46560.1"
                     /translation="HDHNLLTPDVIERSNRNRRRPGIIKPIMAVRNALPSWEADFAPH
                     LSAITHPTMIIWGNDDRVFPLAVGEELHHRIRGSQFVKIPDAGHMPQWERPDLVNRSL
                     IAYIQPSP"
BASE COUNT         1743 a         2143 c         2498 g         1775 t
ORIGIN      
        1 catgatgaca cgaccgaaga tcacagtggt aggtgcgggg aacgtcggag ggacgacggc
       61 gcagcggtta gccgagaaga atctctacga cgtggtattg gtcgatatag cccaaggggt
      121 tccacagggc aaggcgctcg atatttcaca ggcggggccg gtctgtgggt atagcacgca
      181 ggtggtcggc accaatgatt acgtggaaac tgccgggtca tcgatcgccg tgatcacgtc
      241 cggcattccg agaaagccgg gcatgagtcg agacgagttg ttggcgacga atgcgaaaat
      301 cgtgaaatcc gtcgtcatgg agctcgtctc acgctctccg gacattatcc tgatcctcgt
      361 gaccaatcca ttggatgcca tggtccatgt ggcgcgttcc gtcagcggtc tccctaaatc
      421 gagaatcatc gggatggccg gagtactgga ctcggcaaga atgcggacgt tcattgctgc
      481 tgaactcaac gtagaggcta cggaggtgca ggcaatggta ttgggcggac acggcgatac
      541 gatggtgccg ctgccgcgct atacgaccgt gagggggagg ccggtgtcag aattgatgtc
      601 gaaggagaaa cttgacgcca tcgtcaaacg aacgcgggag ggtggagccg aaatcgtggg
      661 ccttttgaaa acgggtagtg ccttctatgc tccctccgct tcagcggtag cgatggtcga
      721 atcgatccat aaggacgaga agcaggtgat gccctgtgcg gtgttgtgtg aaggggagta
      781 cggacttaaa aacgtcgtcg tcggggttcc ggtgaaaatc ggacgcggag gggcggaaca
      841 gattctggag tatgaactga cccgtgatga acgggcggcg ttggaaacct cggccggcgc
      901 ggtgcgggaa ctgtgtgccg cggtcgatcg gttgatgaca taggatgggt gagtggtcat
      961 tagtaagaga aagaaaacga gtccctcatc attgacgagc gcaatgacaa gtgaccgctg
     1021 accggccgac agacatggcg ccttgacaat cggagaaaag ctacagtaca gacatccggc
     1081 tagcagattg cggaataaca tcaggatgct caaaggctgt ccagcaagac cgcagcgagc
     1141 gaaggggcga ggcgtacgct tcggtacgtc gagcctctga acgacgcgag aacgccgctg
     1201 gcggactttt tcagcatcct gttagagagc gcggactgct gacgcgttcg gaaacaaagg
     1261 caattgagac cgatgacaac tgaaacgact cagacttcaa cggactgcgt acagctcaag
     1321 attgttccga tccttgccaa ggagctcggt gtcggggcgc atcagatcag tgctgtcgtg
     1381 acgctgctcg atggaggggc cacggttccc ttcatcgcgc gttatcgtaa agaggcaacc
     1441 ggcaacttgg acgatacgca gctacgtttg ctggaagaac gcttgtgcta tctgcgcgaa
     1501 ttggaggagc ggcgtgtggc cgtgctggcc tcgattgagg agcaaggcaa gctcacggat
     1561 gcgctgcgta ccagcatcga ggccgccacg accaagcaag ccgtcgaaga cctgtacctg
     1621 cctttcaaac ccaagcgacg cacgcgcgcg cagatcgcac gagcggcggg gctcgagccg
     1681 ttggccgaca gcctgttggc cgatcccatg ctgcaccccg agcaagaagc gcttaagtac
     1741 attcgtgtcg tgcccgctgc agaagggatt gaggcggtca atgtgcctga tgccaaaact
     1801 gcgctggaag gagcccgaga tattctcatg gagcggttcg ccgaaacggc cgacttgctg
     1861 gctgcggtgc gggcccggct gtgggaccag ggggtcttga cttcgacggt gatgaaagac
     1921 aaggaaacgg ccgaggagga gaagttccgt gattactatg cctatgcaga gtcgatcaag
     1981 accgtcccgt cgcatcgggc cctcgcgctg ttccgaggtc gtatgctcgg ggtgttgaag
     2041 ctggagttag ggttgggaga ggcgcttgag gcggttgtcc cgcatccctg cgtcgcgatg
     2101 atcgcggctc atgccggcgt cgaagatcga ggccgaccag cggataaatg gctggccggc
     2161 gtatgcgaat ggacatggcg tgtcaagctt cacatgacgc tcagtaccga actgttcgtg
     2221 cagttgcgcg agtcggccga ggccgaggcg atcaagattt ttgcacggaa tctccacgag
     2281 ttgttgttgg cggcaccggc cggaccaaag gtcatcctcg gcctggatcc cggcattcgc
     2341 accggttgca aaatcgcagt ggtcgacgcg acgggaaaat tattggaaac ggagacaatc
     2401 tatccgcacc aacctcgcaa tgaccggcag ggagccgtgg agacgttggc ccgtctggtc
     2461 attcgcaatg gcgtggaatt ggtggcaatc ggcaacggaa cggcgagccg ggagacggac
     2521 aagttggtag gtgaggtgat caaactgatc gcgactcaga agcctgacca caagctggcg
     2581 aagatagtgg tcagtgaagc cggagcgtcg gtctattcgg cgtctgcctt tgccgcggcg
     2641 gagttccccg agctggatgt cagcctgcga ggcgcggtgt ctattgctcg gcgcgttcag
     2701 gacccgctgg ccgagctggt caagatcgag ccgaaggcaa tcggagtcgg gcaatatcag
     2761 catgatgtcg atcagcgggc cttggcgcgg tcgctcgatg ccacggtcga agattgcgtg
     2821 aatgccgtcg gtgtgaacgt caatacggcg tcgatgccgt tgttggaacg ggtctcgggc
     2881 ctcaataagg cgctggccag gaatattgta gactatcggg atgcgaacgg accgttccca
     2941 aatcgtgctg cgatccgcag cgtgccgcgt cttggtgaaa agacttttga acaggcggct
     3001 ggtttcctgc gcatccctga cggcgacaat cccttagatc ggtccgccgt gcacccagag
     3061 gcctatccgg tggttgaacg tattctgacg cggttgggag cggcgatcgc tgaagtgatg
     3121 cgccggccga tggtcttgaa agaattgtca ccggccgact ttattgatga gaagtttggt
     3181 ttgccgacgg tgcgggacat ttttaccgag ctggaaaaac caggccgaga tcctcgtccc
     3241 gaattcaaga cggcgacgtt ccgtgaggga gtcgagtctg tgagtgactt acagcccggc
     3301 atggtgttgg agggggtcgt cacgaatgtg gcggcgttcg gagccttcgt cgatatcggg
     3361 gtgcatcagg acgggctcgt gcacgtgtcc gcgctggcca acaaattcat caaggatcca
     3421 cacgaggtgg tcaaaccggg gcaggtggtc agggtgaagg tgatcgatgt cgatctgaag
     3481 cgccagcgta tcgccctgac catgcggttg gaagatacag cgagccgccc agctggttca
     3541 gcacaatctg gaggcgaagc tatacgtgac tcgcgcggtc gtggaccatc ggcctccggc
     3601 caggcagctg cacgagcttc acaaccgatc ggcgccatgg ccctggcatt ggcgaaggcg
     3661 aggtggaagt catagcgacc gacattcttc gtgaggcatg cgcttgcgcg acaaacagat
     3721 tccctccctt gttgacggca ataattcctt acgatacagt aatacctggc agtagaagaa
     3781 aggaatcgtt atgatcgtca cactgaccag caagggccag acgaccattc ccaaaccgat
     3841 tcgagaccat ctcggtcttc acgccgggga ccaactggat tttgtcattg agccggatgg
     3901 gcgtgtgacc ctgcgccccg ccaccatcga cgtaatggat ctcgccggcc tcttgcgcca
     3961 aaagggtaga aaggcgctct cgcttgaaga gatgcagcga ggggttctga aaggggcgac
     4021 cagacacacc cgatgatcgc gattgatacg aatgtgctcg tacggttcct cacgcaggac
     4081 gaccccaacc aaactcgccg ggtacagcgg tgctttgcga ccgcaaaaga tcaggacgaa
     4141 tccctatttc tcaatcacat cgttctctgc gagtgtgttt gggtgctcgg gtacagttac
     4201 ggctttcaca aagccgccct tgtcgacgta ttggatcgac ttctcgccac caagcagttc
     4261 gtcattgaag acaaaccgtc ggtgtgggct gcggttgccg actaccgcgc ctcacaggct
     4321 gatttttcgg attgcttgat cggcattaag aatcgcaacg cgggctgtga gaccacggtc
     4381 acgttggatc aagggactgc gaagctcgcc acctttcgtc ggctataact tcgggcaact
     4441 tcctgaactt gagaagattt cttcgcagct gcgaaggata tttgaggaag ggattcaaaa
     4501 acaaggcagt tggcaacctg aaccggaaga tgcgggccgc atgcagtctc agagtcagcc
     4561 ggccagcagc tcggttcttc aatgcgcgag cggggtggcg cgagcttttg atcgtcacag
     4621 agagtagaga ttgatcatga gtcgactgtt ctttgccaac gctcaggctc cacttattga
     4681 agttctcatc tccttcgagc agcaggatga cctgagcgtt ctcgggggac ccgatcgatc
     4741 tccaactgcg cgcatgcgac gagcaccgcc cgttcagatc ataattcgat catcattgtg
     4801 tgcgcgttgc gcgaacactg gagatcgacg ggtcacccgt cctttctaca ccggccactt
     4861 ctcctcgttc cacgccatgt cccagaacat ccactcgtac cgtgagctga tgatgaagga
     4921 gtcttccatc cgctggagtg cttctttgcc ggcggtcttg gcccactgat cgactttctt
     4981 tcgcatccag agttggacct cggcaaattc cggcgaggca tagagcatca gccaatcgcg
     5041 gtagggatga ttcttggcag gtggaccttt ccgcaacaag tgttgtccga cgacacagta
     5101 gacccaggcg caaggaaggg cgacgaccgt aatctctgcc gcggtgcctg aagccgcgat
     5161 tgccagcatg tgtctggtgt atgcataatt tgtcggcgcc atcgggacgg atgtcatctg
     5221 tttggccgtg agcgcccatt tctttccata gctttcgtgc agactccgct ctacgatgat
     5281 ggtctcttct gccagcttgt tgaaccgcaa ggccgattcc gaatcgggag ccttcaatgc
     5341 cgcggcagat aacacacgtg ccaaatcgcc aagaaaccgt gcgtcctgca gaatgtaata
     5401 tttgaattta tgttcaggca gtgtgccgtt gccgagggcc acgacgaagg gatggcttaa
     5461 ttgagcatcc cagattgatt tgccaagctt ccgtaggtgg tttgtgaatg acataatgcc
     5521 ctttctcaat gatcctggta gctcgacaga gtgatgggtt gaaacaccgg cgtctagagg
     5581 cacctcatgg cttgaaacgt atggaaccca gggcctcggc atccgattca tgccgtccag
     5641 cgccgcgact ttataacatt ccgccaacgt cgggtagttg aagaccgtat cgataaaata
     5701 atcgattttg ccatgaaagg ccatgacggc ttggccgatg tgaatcagtt ccgtggctcc
     5761 ctcgccgatg acatgaacac cgagcagatg cctggtgtgt cgatgaaaca gtagctttaa
     5821 catgccggtt tcatcgccga tgagctgtcc tcgggcgatt tctctgtagc gggcgatgcc
     5881 gactccataa ggcacctccg cctgtctgag ttcttcctcg ttccgaccca ccaaggagat
     5941 ttccggaatg gcaaagatgc cgtagggaag gagcgacgtg tcggtacggt cgggatagcc
     6001 gaaggcgtga caggcggcgt gacggccctg ttgcatcgat gtggaggcga gggctggaaa
     6061 gccgatgaca tcgccgaccg cataaatgtg gggactcgtc gtttggaagt gttcattgac
     6121 ggtcagacga ccgcggctat cgggttgtaa gccgactgtt tggagattca gcgattgtgt
     6181 cgcgccgacg cgcccgattg catacatgag cgtcgaagct cgaatcggac cgttgtggcg
     6241 aagggacaca tggatggatt tgttccattc tttcttgatc gccaggactt cttcatcgtg
     6301 atacaacgtc actccgaggt ctttcatacg ttgttggagc gcgtcaatga tctcagcgtc
     6361 gacgaactcc aacagccgtg gtcgtttatc gatgagcgtg acggggattc ccaatgcggc
     6421 gaggatcgaa gcatattccg ttccgatcac accgcctccg acgatcacaa tggagtcggg
     6481 aatgcgtttc agcatgagga gaccgtccgt atcgataatg gttttatcgt cgaacgggac
     6541 gtctggaggt ctggctggtt ctgtccccac ggcgatgacg atgaagtccg ccgtcagttc
     6601 aagcgcttcg tgatcccctt gaatgcgcag acgatgtgga tccaaaaagt cggctgaacc
     6661 gaaaaacata tcgacctgat tccgagccat ctgattctgc acggtctgga cttcgtgctt
     6721 gatgacgtgg ttcgcgcgga aggcaaggtc ttcgatcacg accttctttt ttacgcggta
     6781 ctcgtctccg tagatgttgc gctggcgaaa tcccaagaga tagagtaccg cttcgcggag
     6841 ggatttgctc ggaatggtcc cggtattgat gcagacccca ccgacgaccg tctttcgctc
     6901 gatgatcccg accttcttgt tgagcttggc tgcttggatc gcagcctttt ggccggcggg
     6961 tcccgtgccg ataaccagta ggtcgtaatg ggccattggt atgggagagg ttcaggctga
     7021 ggctaaggtt gagactggat catctgcgcc gctcaaacct catcctcaac cttaaccgta
     7081 acctgggtat tacgcaagct cagctctccg tgaccaatcg acgagcaaat tgtagcgctt
     7141 cctccatatc cgggagttga ctgagctccg gggtgatttg cagatagcga atggtgttcg
     7201 ccttatcgac gaccatgacc gctctcgtca aaatgtgcgg accttccaag aagagaccat
     7261 gggctttccc gaattcgccc tcccggtagt ctgacaagaa ggtgacgttg gcgattttgg
     7321 cctcttgggc aaatcgtttc tgggcaaagg gcgtatccac actgacggtg atgagttcca
     7381 ccatcttatc gaggccttta ttccgctcgc tcagaagatg cgtttgttgt tcgcagaccg
     7441 gggtatcgag cgaggggacc acactgatga cgcggacttt tcccgcgcct ttggtctccg
     7501 cgatgtttac gagagataag tcgttctgcg ccaccttcac gtcgcgcagc atgtcgccga
     7561 ctttcactcc gttgcccgcg agtgccagtg gattgccttt gaataggacc ttgttgcctt
     7621 ccccggcgac tgcgctccca tctgccaccg gcatattctt gtaggaaaag ccggcgccgg
     7681 ttgatcctcc cattgcctga catccgacga ggccaagcga aaggcacaga gtaccgatga
     7741 acccagcata ttgtgtccgc atcatacgct cctttccgaa gttcatgagt ggtgaattcc
     7801 tctcgatcta tccattctat ccattcaggg tgacggttga atatacgcga tcaatgatcg
     7861 gttgaccagg tccgggcgtt cccattgcgg catatggccg gcatcgggaa ttttgacgaa
     7921 ctgtgatcca cggatcctat ggtgaagttc ttcacccact gccaagggga acacccggtc
     7981 gtcgttaccc cagatgatca tggtggggtg agtgatagcg ctgaggtgtg gagcaaaatc
     8041 ggcttcccag gacgggaggg cgtttctcac ggccatgatg ggtttgatga tgccggggcg
     8101 tcggcgatta cggttggacc gttcgatgac gtccggcgtg aggaggttgt ggtcgtgga
//
LOCUS       DATMTI010000695         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_26321, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000695 DATMTI010000000
VERSION     DATMTI010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26321"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VN039_11175"
     CDS             <1..348
                     /locus_tag="VN039_11175"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWV46561.1"
                     /translation="YLREAVPNLTFSTLAATPHLYQDRLVLSGAVIVKEEMRDGDLWL
                     HVKNRPLNENYRPQLPPSPNDHEGGWYWIVVNKHQTLPRSYRHWADMTIVGRVTGADP
                     EGQPLLQLVYARG"
     gene            573..1163
                     /locus_tag="VN039_11180"
     CDS             573..1163
                     /locus_tag="VN039_11180"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWV46562.1"
                     /translation="MLAWIHNYEYPAIVLLLMLGIVGLPIPDETLLLFVGYLSFKGEL
                     STPLSLSAAVLGSTCGITVSYGLGHLLGARVPTTLGPWLHINQTRFLTAQRWVSQWGK
                     YGLLVAYFVPGLRHLGALTLGAAGLRYPTFAIFAYTGALVWATTFITAGYLLGEQWTA
                     SSATLHQIFQWIGIGAVITVVGLIMVVMLRRSSNRS"
     gene            1355..3466
                     /locus_tag="VN039_11185"
     CDS             1355..3466
                     /locus_tag="VN039_11185"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWV46563.1"
                     /translation="MLERLIRVSLEKKLAVGLGAALILLLGIGIISYRSTADLIVRES
                     RVVQTHEIRETIEQLLLLMEDLESRQLIDLLTGERQYLQSYREKSQNIEAALRTLADQ
                     TLDSQVQRQYLLTLRRLIDLRVTQFRTLIDLKDAEKIDIDEMKLRLDAGTETMDKIKV
                     VLGKMREQEQMLLAGWSKQTDSAAAFTLSLIFGGTLLTIILASGGGLVIFYDLAKRRQ
                     AEKAVTVGHARQALILRSLPIVMYSAKPSGDYGASWVSENSETVTGYPPKSFVDNSSL
                     WASRLHSDDQEKTLMEFSKLSETGALATEYRWQTHDGEYRWFRDQAVLIRRSDGTPQE
                     LVGLLADVTAQRQANELIRRQADIINQLQETVITVDMNGYVTSWNNGAERMLGYTIKE
                     ALGKHISFVYPADDREFLEREVLTPVKTKGTHQLEIRRRTKSGALRFAHLSLTLLRND
                     SGAPIGIVGCSMDITDRKRGEEALLNSRNQLAALAVRLESVREEERGRIALEIHDVLG
                     QALTGLKLDVSWVYKQILESYGSFEPSAVLSRLASSLELVDSTIQSVREIATTLRPGV
                     LDQLGLEAAVEWQAREFRHRTGIACTTSISPDRIAVGGQQSTALFRILQEVLTNVARH
                     AHASEVDIRLEETREQVIMQIRDNGKGITAAEKSGPTAFGLLGMRLRAQQQDGAFDIQ
                     GTPGIGTTVTVRMPRSRASDD"
BASE COUNT          817 a          963 c          990 g          765 t
ORIGIN      
        1 tatctgcgtg aggccgtccc aaatctcaca ttttcgacgc tggctgccac acctcatctc
       61 tatcaagatc gccttgtcct atcgggggcc gtcatcgtga aagaagaaat gcgggacgga
      121 gacctctggt tgcatgtgaa gaatcgcccg ctcaatgaga actaccgtcc acagcttcca
      181 cccagtccaa acgatcatga aggaggatgg tattggatcg ttgtgaacaa acatcaaacg
      241 cttccacgtt cttaccgcca ttgggccgat atgaccatcg tcggtcgtgt caccggcgcc
      301 gatcctgaag gccaaccgct tttgcagttg gtatatgccc gagggtaggg aatgacatcg
      361 gagcatgatg gagtgtggga gcatactgtg gatgcggact atggagcagc gacgccgccg
      421 aagctcatcc gggagtcgga ccaattccag taatatgcgc accgaagact tttcacacat
      481 gaatccgata ctacaaccga caggcacccg gcgaaggcgt ctcgattgtt gctgatgaat
      541 taaaagacaa tgggcgggac accatggaga caatgcttgc ttggatccac aactatgaat
      601 accctgccat tgttctgctg ctcatgttgg ggattgtcgg cctcccgatc cccgatgaaa
      661 cactgctgct atttgtcggt tatttgagtt ttaaaggtga actgtcgaca ccgctctcac
      721 tgtcagccgc cgtgctgggc agcacctgtg ggatcaccgt cagttacggc ctcggtcatc
      781 tcttgggtgc acgcgtcccc accacactag ggccatggct ccatatcaac cagacacgct
      841 ttcttactgc acaacggtgg gtcagccaat ggggaaagta tggactcctt gtcgcctact
      901 ttgtacccgg attgcggcat ctgggcgcac tcacgttagg ggcagccgga cttcgatatc
      961 ccacctttgc aatttttgcc tacacgggcg cgttggtctg ggctacaacg ttcatcacag
     1021 cgggttatct tcttggtgag caatggacgg cctcttccgc aacgcttcac caaatattcc
     1081 aatggatcgg cattggagcc gtgattaccg tggtgggact gatcatggtg gtgatgcttc
     1141 gacgttcgtc aaataggtca tagaaattcg accgcactac agcatctcgc catggtggac
     1201 ggcaatcgaa ggcggcggcg ccggcatcgg cacctgaatc aggattcagt ggatcgtgat
     1261 cgcgtggtgt gacgcatgcc ataccaaggg gcatccgaac caggcacgtg ttgagtcgct
     1321 gccctactcc acacgtcggc aaggaggcag gtcaatgctt gaacgtctga tccgggtttc
     1381 gcttgagaag aagctcgcgg tgggtctcgg tgccgcgctc atcctcttgc ttggaatcgg
     1441 gatcatttcc tatcgtagta cggccgacct catcgttcgt gaatcccgag tcgtccaaac
     1501 gcatgagatc agagaaacga ttgagcagtt gcttctcctc atggaggacc tggaatccag
     1561 acaactgatc gatctgctga caggtgaacg gcaatacctg caatcgtatc gtgagaagag
     1621 tcagaacatc gaggccgcct tgcgcaccct tgccgaccag acgttggaca gtcaggttca
     1681 gcgtcaatac ttgctgacgt tacgccgtct gatcgatctg agagtgacac aattccgaac
     1741 attgatcgac ctcaaggacg ctgaaaaaat cgacatcgat gagatgaagc tgcgcctcga
     1801 cgccggcacc gagacgatgg acaagatcaa agtcgttctc ggtaagatgc gggagcagga
     1861 gcagatgtta ttggccggct ggtcgaagca gacggacagc gcagccgcat tcacgttgtc
     1921 gctcattttt ggaggaaccc tcttgaccat tatccttgcc agcgggggag gactcgtgat
     1981 tttttacgac ttggcgaagc gccgccaggc tgaaaaggct gtaacggtag gtcacgcccg
     2041 ccaagccttg attttgcgca gtttgcccat tgtgatgtac tctgcaaagc cttcaggaga
     2101 ctatggtgcc tcgtgggtga gtgagaacag tgagacggtg accggttatc caccaaaaag
     2161 ttttgtggat aactcatccc tctgggcatc gcgtcttcat tccgacgatc aggagaaaac
     2221 tctgatggag ttcagcaaac tgtccgaaac cggcgcgctg gcgaccgaat accgctggca
     2281 gacacacgac ggcgagtacc gttggttccg agaccaagcg gtgctgatcc gacggtcgga
     2341 cggaacccca caggaactcg tcggcctcct ggccgacgtc accgcacaac gacaagcgaa
     2401 tgaactcatt cgtaggcagg ccgacatcat caatcaactc caagaaacgg ttatcacggt
     2461 cgatatgaac ggttatgtga cgagctggaa taacggggcg gaacgaatgc tcggatatac
     2521 gataaaagag gccctcggca aacatatttc atttgtctat ccggcggatg accgcgagtt
     2581 cctggaacgt gaggtgctta cccctgtaaa aaccaaagga acccaccaac tcgaaatccg
     2641 gagacgcacc aagtccggag cactacggtt tgcccacctt tcgctcacct tgctgcggaa
     2701 tgacagtggc gctccaatcg gcatcgtcgg ctgttccatg gatatcaccg atcgcaaacg
     2761 cggagaggag gcgctgctta attctcgcaa ccaacttgca gcgctcgccg ttcgcttgga
     2821 gtcggtgcgt gaagaagaac gaggaagaat cgctttggaa atccatgatg tgttggggca
     2881 agccttgaca ggcttaaaac tggatgtgtc gtgggtttac aagcaaattc tcgaatctta
     2941 cggctcgttc gagccgtccg ccgtcctgtc aagactggct tcgtcgttag agctcgttga
     3001 ctccaccatt caatcggtgc gtgaaatcgc gacgacgttg cggcccggtg tgttggatca
     3061 gctcggcctg gaagccgcgg ttgaatggca agcgcgtgag tttcgccatc ggaccgggat
     3121 tgcctgcacc accagtattt cgccggaccg cattgctgtg ggggggcaac aatcgaccgc
     3181 gctcttccgc atccttcaag aggtgttgac gaacgtggcg cggcatgccc atgccagcga
     3241 ggtggacatc cgactggaag aaacccgtga gcaggtgatc atgcaaatca gggataacgg
     3301 caaaggcatt accgccgccg aaaagtccgg gccaacagca ttcggcctgc tggggatgcg
     3361 cctacgcgcc caacagcagg acggtgcgtt cgacattcaa ggcacgccgg gaatcggaac
     3421 aacagtcacc gtcagaatgc cgcgatctcg ggcaagcgat gattaacatt cttcttgcgg
     3481 atgatcatcc gtacctgcga cggggcctga cccagattct caccgatgag ttctc
//
LOCUS       DATMTI010000696         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_536035, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000696 DATMTI010000000
VERSION     DATMTI010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536035"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /gene="ligA"
                     /locus_tag="VN039_11190"
     CDS             complement(<1..1432)
                     /gene="ligA"
                     /locus_tag="VN039_11190"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWV46564.1"
                     /translation="MRQDSLYDDSPSSSRATRAEQERLAQLKSQIRHHDYLYYVKDRP
                     EISDGEYDRLFRELTDLERTYPELVTPDSPTQRVGASPLDELGKVQHERPMLSLDSLA
                     NPEEVLAFDQRMKRELGTDQVEYTVEPKFDGLSVEMVYDHGTFVRGSTRGDGNVGEDI
                     TINLRTIRSLPLQLRAGTYPDRLVARGEVYMRLSDFHALNRRMTERGNDAFANPRNAA
                     AGALRQLDSQITASRPLVVTCYEVMTVTAAPPASHWAELEALARWGLPVPTLRRRCET
                     IDQVLAFHRETENQRDNLPYEIDGVVVKVDRVDWQARLGMKSRSPRWAIAFKFPPRKE
                     ITEVQDIVISVGRTGTLTPLALLKPVEVGGVTISRATLHNADEVARKDIRIGDTVRVE
                     RAGDVIPAIAERIPVANEVRSDPFQMPDHCPICGSAVAREGAYFYCTGQAVCPAQLKG
                     AIEHFASKTALNIEGLGKKTVAQLVDQ"
BASE COUNT          344 a          468 c          475 g          360 t
ORIGIN      
        1 cttgatcgac taattgagcg actgttttct tcccaaggcc ttcaatgttc agggcggttt
       61 tcgacgcgaa gtgctcgatc gcgcctttca gttgagcagg acagacggct tggcctgtgc
      121 aatagaagta ggcgccttct ctcgccacgg cggaaccgca tatcggacag tgatccggca
      181 tctggaaagg atccgacctg acttcatttg cgacagggat acgctcagca atcgccggaa
      241 tgacatcgcc tgcgcgctcg actcgaaccg tatcgcctat gcggatgtcc tttcttgcca
      301 cctcatcggc attgtggagg gttgcgcggc taatggtgac cccacccact tccaccggct
      361 tcagcagggc gagaggggtt aaggtccccg ttcggccgac tgaaatgaca atgtcctgta
      421 cctccgtaat ttcctttcga ggaggaaact tgaatgcgat ggcccaacga ggacttctgg
      481 atttcattcc cagccgcgct tgccaatcca cgcgatcgac tttcaccacg actccatcaa
      541 tttcataagg gagattatcg cgctggttct ccgtttcccg gtgaaatgcc aagacctggt
      601 ctatggtctc gcatcgtcgg cgaagggtag ggaccggaag cccccaccgg gccagtgcct
      661 ccagttcagc ccagtgcgac gcgggaggag ctgcggtcac cgtcatcact tcatagcaag
      721 tgacgacaag cggccgagag gcggtaatct gcgaatcgag ttgccgaaga gcgccggcgg
      781 cggcgttgcg ggggtttgca aaggcgtcgt tcccgcgttc cgtcattcgg cggttgagcg
      841 catgaaagtc ggagaggcgc atatacactt ctccccgcgc gactagacgg tccgggtagg
      901 tgccggcccg caactggagc ggtaacgatc gaatcgtgcg gagattaatg gtgatatcct
      961 ctccgacatt gccatcacca cgggttgaac cacgaacaaa cgtcccatgg tcgtagacca
     1021 tttcaactga tagaccgtcg aattttggct cgactgtgta ctcaacttgg tcggtgccca
     1081 gttcccgttt catccgctgg tcgaaggcca ggacttcctc aggattcgcc aacgaatcaa
     1141 ggctcaacat aggccgttcg tgctgtacct tgccgagctc gtccaaaggg gaggcaccga
     1201 cgcgctgggt cggagagtca ggggtgacca gttccgggta cgtccgttcg agatcggtca
     1261 attctcggaa taaccgatca tactccccat cggaaatctc ggggcggtcc ttgacgtaat
     1321 acaggtagtc atgatgcctg atttggcttt tgagttgagc gagtcgctct tgctcggcgc
     1381 gtgtggctct cgaagaggac ggagagtcgt catagagtga atcctgtcgc atcgctgctc
     1441 tcaacgagat caaatctcga cgatcatctc taaggtgcca cgacggatga cgctcggcat
     1501 gaaaggagag aggcctgaac cccgctgacc ctagcatagg gccgcggaaa cggtcaaacc
     1561 ggacaggccc ggtccgcttt gactttcgca tcagtgacca ctattctaag cgtctcttcg
     1621 gcaggtctga agtcgccgga aagcctg
//
LOCUS       DATMTI010000697         7537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_662719, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000697 DATMTI010000000
VERSION     DATMTI010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662719"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /gene="rpsB"
                     /locus_tag="VN039_11195"
     CDS             <1..344
                     /gene="rpsB"
                     /locus_tag="VN039_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWV46565.1"
                     /translation="QKYLSGIKNMRSLPAAVFVLDTRVEKIAVQEAKRLDIPVIAILD
                     SNCDPDNIAYPIPGNDDAIRSIKLITSKIADACIEGAHVKAQREEAEFPATPASGEKK
                     PAMRVESVPVS"
     gene            406..1002
                     /gene="tsf"
                     /locus_tag="VN039_11200"
     CDS             406..1002
                     /gene="tsf"
                     /locus_tag="VN039_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWV46566.1"
                     /translation="MAGSSQLVKELREKTGAGILDCQKALNENGNDVEKSVDYLRQKG
                     LAAAAKKAGRETNQGLIHSYIHMGGKIGVLIEVNCETDFVARNEEFKAFVNDLALQVA
                     AAKPSFVKREDVPADVAEKEKTIYEGQAMEMGKPPAAWPKIIEGKLEKFYQENCLLEQ
                     SFIKDPAITVKDLLAQKISKIGENMNIRRFTRYQLGEA"
     gene            999..1724
                     /gene="pyrH"
                     /locus_tag="VN039_11205"
     CDS             999..1724
                     /gene="pyrH"
                     /locus_tag="VN039_11205"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWV46567.1"
                     /translation="MSSAKYQRLLLKVSGEMLAGEQGYGIQPSILENLAEEIASVVAL
                     DIQVAVVIGGGNIFRGIAASASGMERASADYMGMLATVLNALALQNALERIGIMTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKSRVVIFAAGTGNPYFSTDTAAVLRAMEISAQVIMK
                     GTKVDGIYDADPVTNPSAKKYERISFLSILNQKLKVMDSTAISLCMDNKLPLIVFNLK
                     VKGNFKRVALGEPIGTLVTLGDR"
     gene            1745..2308
                     /gene="frr"
                     /locus_tag="VN039_11210"
     CDS             1745..2308
                     /gene="frr"
                     /locus_tag="VN039_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWV46568.1"
                     /translation="MSNAASVRQAFITHMDQSLEHLRKDLSGLRTGRASVALLDGIRV
                     DYYGTMTPLKQIANVSTPEARLITIQPWEPKLIKEIEKAISNSGLGVTPSNDGKVIRV
                     PLPPLTEERRKELTKICKKHGEETNVQIRGFRRDANEELKKLQKDEKLTEDELRKAEQ
                     ETQKLIEQYGQKIDDVIKKKEQEIMEV"
     gene            2338..3471
                     /gene="alr"
                     /locus_tag="VN039_11215"
     CDS             2338..3471
                     /gene="alr"
                     /locus_tag="VN039_11215"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWV46569.1"
                     /translation="MSITPTFLPTVATVDLTALVHNLSQFRRMLSPGCDVIAVVKANA
                     YGHGAIETSRTLIRHGVTRLAVFSTEEGVALRQAGITVPIVILGPIFQEQFGDLFAHQ
                     LTPVVSDPSVLTALGQAATSREVFHSIHLKIETGMGRLGLTQDELVALIRSYKFPSSL
                     RLEGLMTHLADADGSDPDATEEQISRFHTALNVVLEGGFQVPLIHMSNSGGAVRFPSA
                     HFSLVRPGIMLYGYHTLPSTVETPDLRPVLSLKTRIAQLRTIQPGGTVSYNRTFTAKC
                     PTRIAILPIGYASGLSRHLSNRGYVLIRGQRVPIAGLVCMDMMMVDVTTIPGIAVGDE
                     VVLIGQQENERITARDIAEWTGTISYEVLCAISPQIPRIYHSS"
     gene            complement(3542..5050)
                     /locus_tag="VN039_11220"
     CDS             complement(3542..5050)
                     /locus_tag="VN039_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HWV46570.1"
                     /translation="MRASFSKPWIGGSGVHDQEKDEMRALSHWGLPKGGRWYSFRYRV
                     RVRVRQGFALLALAVIVCTVSPVSAQVPRVYGQGAAAAGMGNAFAAQADNPSALHYNP
                     AGMTQLRGVQLMAGTTFVGGTTDFTSSSGTSATGDHDGAFAWPGPGHGYITANLQGLG
                     VSALEKLTVGIGVTTPFGSVMRWPDNSPFRGVTTFTALPLFDIKPTLAYQLLPDLSIG
                     AGADIYTFANFFGEGHAELQSISPGGLAPAGTKLEFNGKGTAPGFNVGALYTALRNGE
                     GQPVANIAVVYRSQATLHLDGALLANGVKIQDATTTLVLPQVITGGIGLWPVRNTERE
                     WKVELNVDYVGWKSVRNLDIHSADGSVIRPQPQNWHGTYAILVGTEYRWLRVDRLPGW
                     EIALRGGYTNQQAQVPDLNFNPAVPSADLHVISTGIGLLCKSNGSFLGLISCGSIGLG
                     PVKAKLVGVDLSYQAFLYEPRTISGNTGLRAVVNGLYDSTLHAGTFSVRASF"
     gene            complement(5001..>7537)
                     /locus_tag="VN039_11225"
     CDS             complement(5001..>7537)
                     /locus_tag="VN039_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46571.1"
                     /translation="SYEFTSYLYEFCERISGDSCFAFKAGAEKVSPIMLALTRPFSLS
                     QIYTMFPYFGNKFTSGSVEFRVVEVTSNTAAVGMRFTDRTLRQFGRYRRRCACLVCQS
                     AQGIMVAMAARIHGLAQAEAIETSCIGNDDEWCQWTIRWQEESRYRKRFWRVTSPLEQ
                     TRPTRPSSESVVGGESAQGAQSSVTTRVESSPHLRQSFAWLLWGGVLGLALMAGVGVL
                     NPTVSLGEVLLVGLCPILAVGIMVNRRLLRESERREALIQEQITFVESRHEELRAAYL
                     EQEQMRVELRRKVAQLTALHRAGLSFGSTFERDALLHQVLESLTHELNYNSAMVSMFD
                     PSAHTVQHIRLIGAPPEVETFAQSCRIPIIDCESPEGTVVLQGRPLLVKDIESIRHSL
                     HPLNQRLAELSGTKALVVVPIKTKDRIWGMLTVDRSHNQSVTEDDLELMTTVASQVSI
                     ALDNASAYQQIEEWNQGLEVKVQERTEALERADRLRAQFLSHVSHELRTPLTSIKGFI
                     QNLLDGLTGPLNEKQQRYLVRMSENSDRLVRMIEDLLDRTRIEAGRLEVHPADVELEP
                     CLADVIEQLKPLAQAKQQSLEFRCADTEVVVWADRDRLIQTVVNLVQNAIKFTPPGGA
                     VSVACELPNHRTAAVVVRDTGPGIASEYLDKIFDPFFRIQQGQRTAPKGLGLGLSIVK
                     TLVELQGGEVVARNHPEGGAELSFTIPIHAVQTPPLLEPHLMGQHILVVDDDTDIQQL
                     LHDRLKAEGYQTFSAFDGRQALATLQSREFDGMILDIGIGQIDGLEVLRRVRLTNQHL
                     PIIMITASGSQELAVRAIGLGAQAYLLKPFDAGQLQQTMDRWFRRA"
BASE COUNT         1791 a         2176 c         2003 g         1567 t
ORIGIN      
        1 tccaaaaata cctgtccggc attaagaaca tgcgcagtct tccggcggcg gtctttgtcc
       61 tggatacgag agttgaaaaa attgccgttc aagaagcaaa gcgactcgat atcccagtta
      121 tcgccatctt ggacagcaac tgtgacccgg acaatatcgc ctacccgatt ccgggaaatg
      181 acgacgctat ccgctcgatc aaattgatta catccaagat cgccgatgcc tgtatcgagg
      241 gcgcgcatgt gaaagcccag cgagaagaag cagagtttcc agcaactccc gccagcggcg
      301 aaaagaagcc ggccatgcgc gttgaaagcg ttccagtttc gtaaattaag acaattgatt
      361 catcaacgac tcatcttcat cgacggagcg aaggaataac gaatcatggc aggatccagt
      421 cagctcgtga aggagcttcg agaaaaaaca ggggccggca ttctggattg tcagaaggca
      481 ctcaatgaaa acgggaacga tgtcgaaaaa tccgtcgact atctgcggca aaaaggcctc
      541 gcggcggcgg ccaagaaagc cgggcgtgaa accaaccagg ggctgattca ttcctatatt
      601 catatgggcg gcaagatcgg tgtattgatc gaggtcaact gtgaaaccga ttttgtcgcg
      661 cggaacgagg aattcaaagc gttcgtcaac gatcttgctc ttcaggtggc ggctgcaaag
      721 ccgtcattcg tgaagcgcga ggatgtccca gccgatgttg ccgagaaaga gaaaacgatc
      781 tacgaaggac aggccatgga aatgggcaag cctcctgccg catggccgaa gatcatcgaa
      841 ggcaagcttg aaaagttcta tcaggaaaac tgcctattgg aacaatcatt catcaaagac
      901 ccggccatca ccgtcaaaga tctcctcgct cagaagatct cgaagatcgg cgaaaacatg
      961 aacatccgcc gattcacccg ctaccagtta ggcgaagcat gagctctgcc aaataccaac
     1021 gcctccttct gaaagtcagt ggggagatgt tggccggtga gcagggttac ggcatccagc
     1081 catccatttt ggaaaacctc gcggaggaaa ttgcttccgt cgttgcgctt gacattcaag
     1141 tggcggtcgt catcggcgga ggcaatattt tccgtggaat tgcggcaagt gcgtccggta
     1201 tggaacgggc ttcggcagat tacatgggga tgctggcgac agtgctcaat gcgctggctc
     1261 tccaaaatgc gctggaacgg atcggcatca tgacccgtgt ccaatctgcc atcgaaatgc
     1321 gccagctcgc ggaggggtac atccgtcgga gagcgatccg ccaccttgaa aagagccgcg
     1381 tggtcatttt tgccgccggc acgggcaatc cttatttctc gaccgatacg gccgccgtcc
     1441 tacgggccat ggagatcagt gctcaagtaa tcatgaaagg gacgaaagtc gacggcatct
     1501 acgatgccga ccccgttacc aacccgtcgg cgaagaagta tgagaggatt tcatttctgt
     1561 ccatcctcaa ccagaaactc aaggtgatgg attccacggc gatcagcctc tgtatggata
     1621 ataaattgcc tctcatcgtg ttcaatctga aagtcaaggg taactttaaa cgcgtggcgt
     1681 taggcgagcc gatcgggacc ttggtgacgc tcggcgatcg ctgatcccat cacgaggtgt
     1741 cttcatgtcc aacgcagcct ccgttcgaca ggcattcatc acccacatgg atcaatcgct
     1801 tgaacacttg cggaaggacc tgtccggcct ccgaacaggg cgggcctccg tcgcactgct
     1861 cgacggtatc cgtgtcgact attatggaac catgactccg ctcaaacaga tcgccaacgt
     1921 ctccaccccc gaagcacgac tcatcaccat tcaaccctgg gaaccaaaac tgatcaaaga
     1981 aatagagaaa gccatatcca attcagggct gggtgtgacg ccttcaaacg acggcaaagt
     2041 gattcgagtc ccgcttcccc ctctgacgga agagcgccgc aaggagttga cgaagatctg
     2101 caaaaaacat ggggaagaga cgaatgtcca gattcgaggt tttcggcgag acgcgaacga
     2161 agaattaaag aagcttcaaa aagacgaaaa gctcaccgag gacgaactgc gcaaggccga
     2221 gcaagagact cagaagttga tcgagcagta cggtcagaag atcgatgacg tgatcaagaa
     2281 aaaggaacag gaaatcatgg aggtctgagt ctgacctcct gatgttcgtt tcttcgcgtg
     2341 tcgattacac caacattcct cccgaccgtc gccaccgtag atctgacggc gctcgtacac
     2401 aatctctccc aatttcgtcg gatgctctcc ccggggtgcg acgtcatagc ggtcgtcaaa
     2461 gccaatgcct acggccacgg cgcgatcgaa acgtcacgga cactgatacg acatggtgtc
     2521 actcgccttg ccgtcttctc gacggaagaa ggagttgcac ttcggcaagc cggcatcact
     2581 gtgccgatcg tcatcctagg accgatcttt caagaacaat tcggcgatct gtttgcccac
     2641 caactcactc cggtagtgag cgatccttcc gtgcttacgg cgctgggaca agccgcaaca
     2701 tcacgcgaag tcttccactc gatccatctc aaaatcgaaa caggtatggg tcggctgggc
     2761 ttgacgcagg acgagctggt ggcgctcatc cgctcataca agtttccttc ctccctacga
     2821 ttagaaggac tcatgaccca tctggccgat gccgatggat ccgacccaga cgcgaccgag
     2881 gaacaaatca gtcgattcca tacagccctg aacgtcgttc tggaaggtgg attccaggtt
     2941 cccctcattc atatgtccaa cagcggtgga gcagttcgct tcccatcagc ccacttttcc
     3001 ctcgtgcggc ccggcatcat gttgtatggg taccacaccc ttcccagtac ggtggagact
     3061 ccagacctaa ggccggtgct ctcactgaag acccgtatcg ctcaactccg aactatccaa
     3121 ccagggggaa cggtcagtta taatcggacc ttcaccgcaa aatgccctac acggattgcg
     3181 attctcccga tcggttatgc aagcgggttg agccgacacc tctcgaatcg aggctatgtt
     3241 ctcattcgcg gacagcgagt tcctatcgcg ggattggtgt gcatggacat gatgatggtg
     3301 gatgtcacca cgataccggg catagcagtc ggggacgaag tcgtgctgat tgggcaacag
     3361 gagaacgaac gaatcacagc ccgcgacatt gccgagtgga ccgggacgat ttcctacgaa
     3421 gttctctgtg cgatcagccc acagattccc aggatctacc actcctccta atccgtccca
     3481 cacagccgtt cgccggtgtc cgcagagaac ggagcaaacc gatgtatgcc agaaacccta
     3541 tctagaaact ggctcggacg gaaaacgtgc ccgcgtgcaa ggtgctatcg tagagtccat
     3601 tcactactgc gcgaagcccg gtatttcctg agattgttcg tggctcgtag aggaacgcct
     3661 ggtacgataa atcgacgccg accagttttg ccttgaccgg tccgagtcct atgctaccgc
     3721 aggagataag tcccaagaat gacccattgc tcttgcacaa cagtccaatg cctgttgaaa
     3781 tgacatgcag atcggccgat ggaaccgctg gattgaaatt gagatcgggc acctgcgctt
     3841 gctggttggt atagccgcct cgtagagcga tttcccagcc aggcaaccga tccactctta
     3901 accatcgata ctcggtcccg accagaatgg cataggtgcc gtgccaattt tgcggctggg
     3961 gacggatgac cgacccatcg gctgaatgga tatccagatt tcggaccgat ttccaaccca
     4021 cgtaatctac gttcaattcc actttccact cgcgttcggt gttccggacc ggccacagcc
     4081 caattccgcc tgtaatgacc tgaggcaaca ccaacgtcgt cgtcgcatct tggatcttca
     4141 cgccgttggc caacaaggct ccgtccaggt ggagtgtcgc ttggcttctg tataccacgg
     4201 cgatattggc caccggttgc ccctctccat tccggagtgc cgtatacaat gctccgacgt
     4261 tgaatccagg agccgtgccc ttgccgttaa attcgagttt ggttccggca ggcgctaatc
     4321 cgccgggcga aatcgactgc agctccgcgt gcccttcacc gaagaaattc gcgaaggtat
     4381 agatgtctgc ccccgcaccg atcgagaggt ccggaagcag ctgataggca agcgtgggtt
     4441 tgatatcgaa taacggcaag gcggtgaacg ttgtgacacc tcgaaacgga ctgttatccg
     4501 gccatctcat caccgagccg aacggcgttg tgacgccgat ccctaccgtc aacttctcta
     4561 gcgccgagac acccaggcct tgaagattcg ccgtgatata tccatgacct ggaccaggcc
     4621 atgcaaaagc accatcgtgg tctcctgtgg cagaagttcc gctcgaactt gtaaagtcgg
     4681 ttgtccctcc gacgaaggtt gttcccgcca tcagttgcac tccacgaagc tgtgtcatgc
     4741 cggctgggtt gtaatgaagc gccgacggat tgtctgcctg tgcggcgaaa gcattaccca
     4801 tcccagccgc agcggctccc tgaccataga ctcgcggcac ttgagctgaa acaggggaaa
     4861 ccgtgcagac gatgacggct agtgcaagaa gagcaaaccc ttggcgcaca cgaacccgaa
     4921 ccctataccg aaaggaatac catcgccctc ccttgggcag tccccaatgg ctcagcgcgc
     4981 gcatctcatc cttctcctgg tcatgcacgc cggaaccacc gatccatggt ttgctgaagc
     5041 tggcccgcat cgaatggctt gagcaggtag gcttgggctc ccaacccgat ggcccttacg
     5101 gcgagttcct gggatcccga tgccgtaatc atgataatag gaaggtgttg attcgtcaga
     5161 cgcactcgtc gcagcacttc cagaccgtcg atctgaccga ttccgatgtc gagaatcatc
     5221 ccatcgaact cccgagattg gagagtcgcc agtgcttgac ggccatcgaa cgccgaaaac
     5281 gtctggtagc cttctgcctt cagccggtca tgaagcagtt gctgaatgtc ggtatcgtca
     5341 tccacgacca ggatgtgttg acccatgaga tgaggctcaa gcaacggtgg cgtctgcacg
     5401 gcatgaatcg gaatcgtgaa ggacagttct gcgccgccct ctggatgatt acgagccacg
     5461 acctccccgc cttgcaactc caccagtgtt ttcacgattg acagtccaag tcccaaccct
     5521 ttcggggctg tgcgctggcc ttgttggatg cggaagaacg gatcaaaaat cttgtcgagg
     5581 tattcagaag caataccggg gcccgtatcc cgaaccacga cagcggccgt gcggtggttc
     5641 ggcaattcgc aagcgaccga cacggctccg ccaggcgggg tgaacttaat ggcattttgc
     5701 accagattga cgaccgtctg aatcaaacga tcgcgatccg cccagacgac gacttcggtg
     5761 tcggcacaac ggaattcgag tgattgctgt tttgcctgcg ccagcggctt cagttgctcg
     5821 atcacatcag cgagacaggg ttccagctcg acatcggccg gatgcacctc caggcgcccg
     5881 gcctcgatac gggtacgatc gagaagatcc tcgatcatgc ggaccagccg atctgaattt
     5941 tccgacatgc gcacaagata gcgctgctgc ttttcattca acggtcccgt cagcccgtcc
     6001 agcagatttt gtatgaatcc cttgatggac gtgagcggtg ttctcagttc atgggacaca
     6061 tgtgaaagga attgcgcacg caaacggtcg gcccgctcaa gggcttcggt gcgctcttgt
     6121 actttgacct ccaagccctg attccactct tcaatctgtt ggtaggctga ggcgttatcc
     6181 aacgcgatgg agacttgact cgcgaccgtc gtcatcagtt ccaaatcgtc ttccgtcacg
     6241 ctttgattgt gcgagcgatc gaccgtcagc atcccccaga tacggtcttt agtcttgatg
     6301 gggaccacca ccaaggcctt ggtcccgctt aattcggcta aacgttgatt gaggggatgc
     6361 aggctgtgtc gtatcgactc gatatctttg accaacaggg ggcgtccttg gagcaccacc
     6421 gtcccctcgg gactctcgca atcgatgatc ggaatccgac aggactgggc gaacgtttca
     6481 acctccggag gcgcgccgat cagacggata tgttggacgg tatgcgcaga gggatcaaac
     6541 atggatacca tggcactgtt atagttgagc tcgtgtgtca aggattccaa cacctggtgc
     6601 aacagagcat ctcgttcgaa ggtcgagccg aatgagagcc ccgctcgatg aagcgccgtc
     6661 agttgggcca ctttccggcg caactccacc cgcatctgtt cttgttccaa ataggctgcg
     6721 cgcaattcct cgtgacggga ttcgacgaat gtgatctgct cttggatcaa ggcctcgcgt
     6781 cgttcgctct cccgtagcag ccgccgattg accatgatac ccacagcaag gatcgggcat
     6841 agtccgacta acaacacttc cccgagactc accgtcggat tcagcacacc gactcccgcc
     6901 atgagggcaa ggcccaacac ccctccccat aacaaccatg caaagctctg ccgcagatga
     6961 ggcgaggact cgacacgggt cgttaccgaa gactgtgcgc cctgggcact ctctccgccg
     7021 acgaccgact cactgctcgg tcgagtcgga cgtgtctgct caagaggtga cgtcactcgc
     7081 cagaatcgct tccgataccg gctctcttct tgccatcgga tcgtccactg gcaccattca
     7141 tcatcattgc cgatgcagga ggtctcgatt gcctccgcct gagcgagacc gtggatgcga
     7201 gcagccatgg cgaccataat gccctgggca gactgacaga ccaggcaggc acagcgccta
     7261 cgatagcgcc cgaactgccg aagcgtccgg tcggtaaacc gcattcccac tgcagccgtg
     7321 ttgctcgtga cttccaccac gcggaactcg acagatccgg atgtaaactt attcccaaag
     7381 tagggaaaca tcgtgtagat ctgcgacagg gaaaaggggc gagtcagggc cagcatgatg
     7441 ggagacacct tttccgctcc agccttgaaa gcaaaacagg agtcgcccga gatccgttca
     7501 cagaactcat acaggtacga ggtaaattcg tacgaat
//
LOCUS       DATMTI010000698         1563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_26381, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000698 DATMTI010000000
VERSION     DATMTI010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26381"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VN039_11230"
     CDS             complement(<1..491)
                     /locus_tag="VN039_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV46572.1"
                     /translation="MNGPVPDISPPEKLTAEHDILGFDSGVSVLDDWFRRRALANEDS
                     GSSRTYVVCVQKSAVGYYALAVGAVAHAGTPGCVKRNMLDPVPVIVLGRLAVDKSFQG
                     RGIGSGLLRDAVLRTVQAAEIAGIRAIAVHAISEAAKRFYQGYGFIPSPINPMTVMIT
                     IAEA"
     gene            complement(488..778)
                     /locus_tag="VN039_11235"
     CDS             complement(488..778)
                     /locus_tag="VN039_11235"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HWV46573.1"
                     /translation="MATMARETGRGETINLRASQKQKAMIDRAAEVLGRSRSEFMLEA
                     ACREAETVLLDCRYFGLSPEAFKRFTAMLDKPPASNYKLRRLLLQTRAPWEE"
     gene            complement(967..>1563)
                     /gene="mtnA"
                     /locus_tag="VN039_11240"
     CDS             complement(967..>1563)
                     /gene="mtnA"
                     /locus_tag="VN039_11240"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HWV46574.1"
                     /translation="GDGQTVLTHCNAGSLATAGYGTALGVIRAAWEQGKKIKVIADET
                     RPVLQGARLTAWELMQDQIPVTLITDNMAGSLMRQGKIHLCVVGADRIAANGDVANKI
                     GTYSVAVLAKAHNIPFYVAAPYSTIDLKTKSGADIPIEQRNPLEVTTIHGSHPVAPKD
                     VPVYNPAFDVTPAELITGIITERGVFKPSEIGERFSLS"
BASE COUNT          349 a          439 c          424 g          351 t
ORIGIN      
        1 gcctctgcaa tcgtgatcat caccgtcatt ggattgatcg gtgagggaat gaacccgtat
       61 ccctgataga aacgttttgc ggcttcagaa atcgcatgga cggcgatggc gcggatgccg
      121 gcgatctcgg cagcctgaac ggtacgcaaa acggcatcgc gtaacagtcc gctgccaatt
      181 ccgcgacctt ggaagctctt atcgacggca agtcgcccga gcactatgac gggaacggga
      241 tcgagcatgt tgcgcttcac acagccaggc gttccggcat gggcaacagc gccgacggcg
      301 agggcataat acccaaccgc actcttttgc acacagacca cataggtgcg agacgatccg
      361 ctgtcttcat tcgcgagagc acgccgacga aaccagtcat ccaagaccga cacccccgaa
      421 tcaaaaccca aaatatcatg ctcggcggtc agtttctcag gtggggagat gtcaggaaca
      481 gggccgttca ttcttcccac ggcgccctgg tctgaaggag gagacgtcgc agtttataat
      541 tactcgcagg gggcttatcg agcatggccg taaagcgctt gaatgcctcc ggagatagcc
      601 cgaaatatcg gcaatcgagc aggaccgttt cggcttctcg gcatgccgct tccagcatga
      661 attccgaccg gctccggcca agcacttccg ccgcccggtc gatcatcgcc ttctgttttt
      721 gactggcacg cagattgatc gtctctcctc gccctgtctc tcttgccata gtggccataa
      781 ctggctgcct ccttgtgttt acaaaaacta cacattagtg tgcacacatt gtaaacacaa
      841 aatcaaccga catgaatgac tttatgcctt gatcggtagc tatcagatta cgtttggaag
      901 gcaagacgga ttcccggcgt cagccagata gcggaaaagt ttcttgctga acgtaagttt
      961 ggggggctat gacagagaga aacgttcgcc aatctcactc ggcttgaaga cgcctcgctc
     1021 cgtaatgatc ccggtaatga gttcggccgg ggtcacgtcg aaggcggggt tatagacagg
     1081 cacatctttg ggtgcgactg gatggctgcc gtggatggtc gttacttcca gcggattccg
     1141 ttgctcaatt ggaatatcgg ctccggattt tgtcttgaga tcgatggtcg aatagggagc
     1201 cgcaacatag aagggaatgt tgtgggcttt cgccaagacc gccacggaat aggtcccgat
     1261 cttattggct acatccccgt tcgctgcgat ccgatcggcg ccgacgacac agagatgaat
     1321 cttcccttgc ctcatgagcg aaccagccat gttgtccgtg atgagcgtga ccggtatctg
     1381 gtcctgcata agctcccatg cggtgagacg cgcaccttga agcacaggac gagtttcatc
     1441 ggcgatcacc ttgattttct tgccctgctc ccatgcagcg cgaatgactc cgagtgccgt
     1501 tccataccct gccgtcgcga gtgagccggc attacagtga gttaaaactg tctgtccgtc
     1561 ccc
//
LOCUS       DATMTI010000699         5276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_323732, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000699 DATMTI010000000
VERSION     DATMTI010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323732"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2456)
                     /locus_tag="VN039_11245"
     CDS             complement(<1..2456)
                     /locus_tag="VN039_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV46575.1"
                     /translation="MSPSRLFIMRPVATALSMVAILLTGVLAYRQLSVSALPQIDYPT
                     IQVSTFYPGASPDVMASSVTAPLERHFGQMPGLNQMTSTSSNGCSVVTLQFSLDLSLD
                     VAEQQVQAAINAASTFLPRDLPNPPIYNKVNPADAPILTLALTSSTLPLSQVEDLTET
                     RLAQKISQLSGVGLVSISGGQRPAVRIRANPRALSAYGLTLEDLRMTVAAANVNQAKG
                     AFHGPQRASIINATDQLLSSREYRPLIVAYRKGAPVHLSDVADVVDDVENVRQAAWMD
                     ETPAVLINIQRQPGANVIEVADRIKKILPQLQSALPSSVQVTMLTDRTTSIRASVRDV
                     QFELILAVALVIMVIFLFLRTLSATVIPAVAVPLSLVGTFGAMYLMGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENISRYIERGESPFQAALKGSEQIAFTILSLSISLIAVLIPLL
                     FMGDVVGRLFREFAVTLSITIVISAVVSLTVTPMMCARLLRHRRATEQGTFYRRTQRA
                     FDRTIAAYGRTLRWVLNHQPATLAVAGGMLLVTIVLYVAVPKGFFPVQDTGAILGISE
                     AAQSISFASMAERQQALARAILTDPAVASLSSFIGVDGINTTLNSGRIQINLKPIAER
                     RVGAQDVIVRLQDHVRKVDGITLFLQPMQDLTVEDRVSRTQYQYTLEDSDSEELRRWA
                     PTLVEALQALPELRDVGSDQQDKGLASLLVIDRSTASRLGITPQLIVDTLYDAFGQRQ
                     VSTMFTQLNQYRVVLEVMPEFQNGPQALQFLDIRSWTGGQVPLNVFTQFSETTTPLAI
                     SRQGQFPAVTLSFNLAPGTSL"
     gene            complement(2453..3682)
                     /locus_tag="VN039_11250"
     CDS             complement(2453..3682)
                     /locus_tag="VN039_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HWV46576.1"
                     /translation="MSESIHFAATLKRWVLGLSAACLLALGGYALLAKSDEKPSRAGQ
                     ASAGARAFPVVVAEAKTGDIGIYLNGLGSVVSLNTVNVKSRVDGQLMKVLFQEGQVVR
                     TGDLLAEIDPRPFEVQLLQAEGQMARDLAQLSNARLDLQRYKELYEQGYVPKQQLDTQ
                     DAMVRQLVGIVKADQSQIDNAKLQLSYSRITASISGRVGLRQVDPGNMVHANDNNGLL
                     IITQLAPIGVVFSIPEDSLPGVLERLKAGERLSVDAFDREQKKKLATGVLLTVDNQID
                     PNTGTVRLKAVFPNEDGALFPNQFVNARLLLEMKHGVTIVPSVAVQRGATGTFVYVVN
                     NDRTVAVRTVAVGASHGEDTSITSGLSPEELIVVDGTEKLREGSKVEVRNQNDQPVKG
                     PDALPTGSEMPQQGTKS"
     gene            complement(3694..4110)
                     /locus_tag="VN039_11255"
     CDS             complement(3694..4110)
                     /locus_tag="VN039_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46577.1"
                     /translation="MSRLKLWVGLIVLFGAGVATGSVGTFLYADAERIHREERGPAAQ
                     HDRIMKRLTQGLSLTTQQQLEIEPILSLAHIALLELRFPHQGEIERILSKGMADIKGR
                     LSSEQQSKLDKMYVELQQRWRLSHDYLEAHKKSAKL"
     gene            complement(4132..4446)
                     /locus_tag="VN039_11260"
     CDS             complement(4132..4446)
                     /locus_tag="VN039_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46578.1"
                     /translation="MQDDEVLKLERALTEVYRSQPEASSSSVEVTQEVMRQIRQLTSS
                     KNSVWVQSSVLDQLVWRTAAITAAVVMIVTILTVETLRTTADESGVLLADEFESAPLF
                     GD"
     gene            complement(4576..5193)
                     /locus_tag="VN039_11265"
     CDS             complement(4576..5193)
                     /locus_tag="VN039_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWV46579.1"
                     /translation="MPAISGIHLLMADGSSFKPSSDEKNLLRCIKQGETERFADLIGR
                     YQPHVIRIVGRRVPADQVKEVVHDVFVRAYFSLAHFSESVAFDHWLAGIAVRTCYDFW
                     RAKKRDEVPVSVLADGHHRWIEQMLAAQSEDQFRDQVQKREAAEVLEWALKQLSPENR
                     AVLTLVHLEGRSVREAAQLLGWSVINVKVRAHRARQAVRKLLAGG"
BASE COUNT         1100 a         1580 c         1493 g         1103 t
ORIGIN      
        1 agagaggtgc ccggggccag attgaatgac agggtgacgg cgggaaactg cccctgacga
       61 ctgatcgcca gcggggtcgt cgtttcggag aactgggtaa acacgttcag cggcacctgg
      121 cctccggtcc acgaccggat gtcaagaaac tggagggcct gcgggccgtt ctggaattcc
      181 ggcattactt cgagcacgac gcgatactgg ttcagctggg taaacatcgt ggagacctgc
      241 cgttgcccaa acgcgtcgta cagggtatcg acgatcagct gaggcgtgat gccgagccgc
      301 gaggcggtac tgcgatcgat gaccaggagg gacgcgagcc ccttgtcctg ttggtcgctg
      361 cccacatcgc gcaattccgg caaagcctgc agcgcctcaa ccaacgtggg agcccatcgc
      421 ctcaattcct ccgaatccga gtcttccaac gtatattggt actgggtgcg gctgacacga
      481 tcctcgacgg tcaaatcctg cattggttgc aggaacaagg tgatgccatc cactttcctt
      541 acatgatctt gcagtcgaac aatcacgtcc tgcgcgccga cccggcgttc ggctatgggt
      601 ttcaggttga tctggatccg tccgctgttg agcgtcgtgt tgatcccatc cacgccgatg
      661 aaggaggaca ggctggctac ggccggatcg gtgaggatgg cgcgggccag cgcctgctgc
      721 cgttcggcca tggaggcgaa tgagatcgac tgggccgctt cggaaatgcc caggatcgcc
      781 cctgtatcct gcacagggaa gaagcccttg ggcacggcga cgtacagcac gatcgtgacc
      841 aggagcatcc cgcccgccac tgccagcgtg gcgggctgat ggttcagcac ccatcggagc
      901 gtcctcccat aggccgcgat ggtgcggtcg aacgcccgtt gtgtccggcg gtagaaggtt
      961 ccctgttccg tcgcacggcg atggcgcagc agccgggcgc acatcatcgg tgtcaccgta
     1021 agcgagacga cggccgaaat cacgatcgtg atactcagcg tgacggcaaa ttctctgaac
     1081 aagcgcccca ccacgtcacc catgaatagg agcgggatta acaccgcgat aagcgagatg
     1141 gacagcgaga gaatcgtgaa cgcgatctgt tccgatcctt tgagcgcggc ttgaaacggt
     1201 gactcacccc gctcaatgta tcgcgagatg ttttcgatca tgacgattgc gtcgtcaacg
     1261 acgaaaccgg ttgaaatcgt gagcgccatt aaagtcaggt tgttgaggct gaaccccatc
     1321 aggtacatcg cgccgaatgt gcccacaagc gacaatggaa ccgccacggc gggaatgacg
     1381 gtagccgaca gggtacgaag aaaaagaaaa atgaccatga tcacgagggc aacggcgagt
     1441 atcagctcga actgcacgtc gcggacggat gcccggatgg aggtggtccg gtcggtcagc
     1501 atcgtgacct gcacggagga cggcaacgcg ctctggagtt gcggcaggat cttcttgatt
     1561 cgatccgcca cttcaatgac attggctccg ggttgccgtt ggatattgat gaggacggcc
     1621 ggcgtctcgt ccatccaggc cgcctgcctg acgttttcta cgtcatcgac gacgtcggcg
     1681 acatcggata agtggacggg cgcccccttg cggtaggcga cgatcagcgg ccggtactcg
     1741 cggctcgaca acaactgatc ggtcgcgttg atgatggagg ctctctgcgg cccatggaat
     1801 gcgcctttgg cctgattgac gttggcggcg gccacggtca tgcgcagatc ctccagcgtc
     1861 agtccgtagg ccgacagggc cctgggattc gccctaatac gcacggccgg ccgctggcca
     1921 ccgctgatgc tgacgagccc cacgccggag agctgggaga ttttctgcgc gagccgggtt
     1981 tcggtcaggt cctcgacctg agacaacggc aacgtgcttg acgtcagagc caacgtcagg
     2041 atcggcgcat cggccggatt gaccttgtta tagatcggcg ggttcggaag atcacgcggg
     2101 aggaatgtgg aggcggcatt gatcgcagcc tgtacctgtt gctcggccac atccaagctc
     2161 agatcgaggc tgaattgcag cgtcaccact gaacagccgt tcgaactcgt tgaggtcatc
     2221 tgattcagac caggcatctg cccgaagtgt cgctcaagcg gcgcggtcac agacgatgcc
     2281 atgacgtccg gactggctcc gggataaaaa gtcgagacct gtatcgtggg gtaatcaatc
     2341 tggggaaggg ccgagaccga caattgccga taggccagca ctccggtcag caaaatggcg
     2401 accatcgata aggcagtcgc cacaggccgc atgatgaaga gccgcgacgg actcacgatt
     2461 tggtgccctg ctgcggcatc tcggatccgg ttgggagagc gtcaggacct ttgacaggtt
     2521 ggtcgttctg gttccgcact tcgaccttgc tgccttcacg aagtttctcg gtgccgtcca
     2581 ccacgatcaa ttcctcgggc gagagaccgg aggtaatcga tgtgtcctcg ccatgagatg
     2641 caccgaccgc aacggtccgg acagccactg tccggtcgtt attcaccaca tagacgaagg
     2701 tccctgttgc tccgcgttga acggcgacgg acggcacgat cgtgactcca tgtttcatct
     2761 ccaacaacaa gcgggcgttg acgaactgat taggaaacaa cgcgccgtcc tcgttcggga
     2821 acactgcctt cagccggacg gtacccgtat tgggatcaat ctgattgtcg accgtcagga
     2881 gaacgccggt ggccagtttt ttcttttgct cccgatcgaa cgcgtccacg gacagtcgtt
     2941 cgcccgcttt gagtcgttcc aaaactcccg gcaaactatc ctccggaatg gaaaagacga
     3001 ccccgatcgg cgcaagctgt gtaatgatca gcagaccatt gttgtcgttt gcatggacca
     3061 tgttgccggg atccacctga cgcaacccca cccgcccgct gatggacgct gtgatgcgac
     3121 tatagctcag ttgcagcttg gcattgtcga tctggctttg gtcggccttc acgatcccta
     3181 ccaattgacg caccatggca tcctgggtat ccagttgttg cttgggaaca tagccctgtt
     3241 catacaactc cttgtaccgc tgcaagtcca gccgcgcgtt gctcaattgc gccagatcgc
     3301 gagccatctg cccctcagcc tggagcaatt gcacttcgaa tggtcgcggg tcgatctctg
     3361 ccaagaggtc gcccgtccgg acgacctgtc cttcttgaaa cagcaccttc atgagttgcc
     3421 catcgacccg actcttcaca ttgacggtgt tcaaggatac aaccgatccg agtccgttga
     3481 gatagatacc gatgtcgccc gttttcgcct ccgccactac caccggaaat gcacgcgcgc
     3541 ctgccgacgc ctgaccggcg cgcgacggtt tctcatcaga cttcgccaag agtgcatacc
     3601 ctcccaaggc aaggagacag gccgctgaca ggccaagcac ccaacgtttc agtgtagccg
     3661 caaagtgaat cgattcagac atggcggtgc cagctagagc tttgcgctct ttttgtgagc
     3721 ttccaaatag tcatgtgaaa gtcgccaacg ctgttgtagt tccacataca tcttgtccag
     3781 tttcgattgc tgctctgagg acaaccgccc tttgatatcc gccattccct tgctcaagat
     3841 ccgttcaatt tccccttgat gcggaaagcg caactcgaga agtgcgatat gagcgagact
     3901 gaggatcggc tcgatttcca gctgttgttg ggtcgtgagc gacagtccct gcgtgagacg
     3961 tttcatgatc cggtcgtgct gagcggccgg gcccctctct tcccgatgta tccgctcggc
     4021 atctgcgtac aggaaggttc cgacgctccc ggtcgccacc cccgcgccga ataagacaat
     4081 caggccaacc catagtttga gtcgtgacat ggctcctctc tttcccagcc actagtctcc
     4141 aaataggggg gccgattcaa actcgtctgc cagcaacact ccgctctcat ccgcagtcgt
     4201 ccggagtgtc tccacggtga ggatcgtcac aatcatcacg actgcggcgg tgatggccgc
     4261 tgtccgccag acaagctgat caagaacaga cgactgcacc cacacactgt ttttgctgct
     4321 cgtcaattga cgtatttgcc tcatcacctc ctgcgtcact tcgacagaac tggatgaggc
     4381 ttccggctgt gaccgataaa cctccgtgag cgcgcgctcg agttttagga cttcgtcgtc
     4441 ttgcatgagg tgctccctcc cccactgttg cctagcgggc cgtttaaaag gccgattgat
     4501 tccccgactg gactccggaa accttcaggc gttctcattc ggcgagcgac acgagcacat
     4561 tattgacatt ccttttcaac cgccggcaag aagtttgcgc accgcttgtc tggcacgatg
     4621 cgctcgaact ttgacattga tcacgctcca tcccaataac tgtgccgcct cgcgtaccga
     4681 gcgaccttcg agatgaacca aggtcaagac agctcgattc tccggcgata gttgtttgag
     4741 cgcccattcc aagacctccg ccgcctctcg tttctgtacc tgatcacgga attggtcctc
     4801 cgattgcgcc gccagcatct gctcaatcca gcgatgatgc ccatcagcca agacgctgac
     4861 gggaacttcg tcccgtttct ttgctctcca gaaatcgtaa caggttcgga cggcaatacc
     4921 cgcaagccaa tgatcgaagg cgaccgactc cgaaaaatgc gccaggctga agtatgctct
     4981 gacgaagacg tcgtggacca cttctttgac ctgatcggcg ggtacccgtc gtcccacgat
     5041 gcggatgaca tggggttgat accgaccgat cagatcggca aatcgttcgg tctctccctg
     5101 cttgatacat cggagaagat tcttttcatc cgacgaaggt ttgaatgacg agccgtcggc
     5161 catcaacagg tgaattcccg aaattgcggg cacctctcgg atcgacaatg cggctcatga
     5221 taccacctcg cgcttgaatc gaactaccga gtcaaccgac cgaacggctg cgcctg
//
LOCUS       DATMTI010000700         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_620078, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000700 DATMTI010000000
VERSION     DATMTI010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620078"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VN039_11270"
     CDS             complement(<1..636)
                     /locus_tag="VN039_11270"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWV46580.1"
                     /translation="MPVDRNRLKADLHRLVGERHPLSSPLHLQEAESYLHRQFTDAGL
                     IVTKHHFQALGGTYHNVIGTVRPATQPCQSAPPLILAAHFDTVQGSPGADDNASALAV
                     MLAVARQVRAMTLARPIRFIGFNLEEENLLGSSAYTALLRKNRETIHGAIVLECVGYA
                     SHQHNSQKIPPGVPIAVPTTGNFLAVIGNERSQALTGSVVQAMKSHLPIVPL"
     gene            complement(655..>1508)
                     /gene="argB"
                     /locus_tag="VN039_11275"
     CDS             complement(655..>1508)
                     /gene="argB"
                     /locus_tag="VN039_11275"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWV46581.1"
                     /translation="PYMRTFRGKTVVVKYGGHAMTDSSLKERFAQNVVLLKYVGINPV
                     IIHGGGPQIDKMLDRLGIEAKFRHGVRITDEATMEIVEMVLAGKINMEITDLINRHGG
                     SAVGLSGKDGGLILSKPLTAKAWAESLDRELDGEDGDGDFGLVGDIEKVDPGLLRNLQ
                     DDHYIPVIAPIGTDREGNTYNINADLVAGAVAGALRAEKLLMMTDIKGIRDANGRHLS
                     TVSRKDVQRMMKKGTITEGMIPKVHACLDALAEGVGKAHIIDGRIPHALLLEIFTHKG
                     IGTEIVN"
BASE COUNT          286 a          416 c          454 g          352 t
ORIGIN      
        1 cagtggaaca attgggaggt gcgatttcat ggcctggacg acagaaccgg tcaaggcctg
       61 tgagcgctcg ttaccaatca cggcgagaaa atttccggtt gtggggacgg cgatgggcac
      121 acctggagga attttctgag agttgtgttg atggctcgca tagccgacac attccaacac
      181 gatcgcgccg tggatggttt cacgattttt tctgagaagc gctgtatagg cagagctgcc
      241 gagcaagttc tcttcttcca agttaaaacc aatgaaacgg atgggtctgg ccaatgtcat
      301 ggctcggact tgtcgggcaa ctgcgagcat cactgcgagg gcgctggcgt tgtcgtcggc
      361 gccgggagac ccctgcacgg tatcgaagtg cgcggcgagg atcagcggcg gtgcggattg
      421 gcatggctga gtggcaggac gcaccgttcc gattacatta tggtatgtgc cgcccaaggc
      481 ctggaagtgg tgcttcgtga cgatcagacc tgcgtcagtg aactgtcggt gtagatagga
      541 ctctgcttcc tggaggtgca acggggacga gaggggatgg cgttcgccga ctaggcgatg
      601 caggtcggct ttgaggcggt tgcgatcaac gggcacgtgg cttccggtac gaaattagtt
      661 cacgatctcg gtcccaatgc ccttatgtgt gaagatttca aggagaaggg cgtggggaat
      721 acgcccgtcg ataatgtggg ctttcccgac cccttcggcc agcgcgtcca gacacgcatg
      781 gactttcggg atcattccct ccgtgatggt tcccttcttc atcatgcgct gcacgtcttt
      841 gcgcgacacg gtggacaggt ggcgcccatt ggcgtcacga atgcctttga tatcggtcat
      901 catgaggagc ttctccgctc gcaaggctcc ggccacggct ccggcgacca gatcggcatt
      961 gatgttgtag gtgttccctt cgcgatccgt tccgatcgga gcgatcacag ggatgtaatg
     1021 gtcatcctgc agattacgca gcaggccggg gtcgaccttt tcgatgtcac ccaccagtcc
     1081 gaagtcgccg tcaccatctt ctccgtccaa ctcgcgatcg aggctttccg cccaggcctt
     1141 ggccgtcaat ggtttgctga gtatcaatcc cccgtccttc ccgctcaacc cgaccgcgct
     1201 gccgccgtgt cggttgatga ggtcggtgat ttccatattg atctttcccg ccaggaccat
     1261 ttccacgatt tccatcgtgg cctcgtcggt gatccgaaca ccgtgtctga atttcgcttc
     1321 gatgccgagc cggtcgagca tcttgtcgat ttggggtccg ccgccgtgga tgatgaccgg
     1381 attgatgccg acatacttca gcaagacaac gttctgcgca aaccgttcct tgagggaaga
     1441 gtccgtcatc gcatgaccgc cgtatttgac gaccacggtc ttcccccgaa atgtgcgcat
     1501 gtacggaa
//
LOCUS       DATMTI010000701         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_280513, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000701 DATMTI010000000
VERSION     DATMTI010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280513"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1541
                     /locus_tag="VN039_11280"
     CDS             <1..>1541
                     /locus_tag="VN039_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV46582.1"
                     /translation="ITYVFERFLTQTVEGIKYIWSNSLFGNSIINIYLQDGVDLGGSE
                     ADIAAIAQTSVKALPPDINAPMVMRLFPSQVPVATLQVTSDSHTPAELYNLSIMRIRP
                     LLVTIPGAILPHPYGGQDMQVMINLDQQKLLARHLTPADIHQALDRQNLVLPGGDIKI
                     KSTDWIVLTNAQPLKIEDFDDIPIKKEGNAWIHLRDVSVTRLAGRVQTNAVIVDGQQA
                     VIIVVMKSSEASTLEVVDGIKEIIPRIQEVVPEGVKVKLLIDASGFVKQAIADVLHEM
                     VIAAGLVGLIVLVLLGSWRPTVIVLTSIPLSILASLIGLHALEQSINIMTLGGLALAV
                     GILVDNAAVMIENIDTHLATGRPVEEAIIDAANQIILPTFVATLAITIVWVPMFHLSG
                     ISGWVFPPMAQAVIFAMIASFILTYTLVPTMAKYILKAHNAHGHGGQSHAEQSRNIFT
                     RFQQGFQDGFERFRERYTMQLERAVAHRGRFVVVSSAVAAACLVLFYFNGREFFPEIR
                     SNILQ"
BASE COUNT          324 a          456 c          422 g          339 t
ORIGIN      
        1 gtatcacata tgtcttcgag cgcttcctga cgcagacggt cgaaggcatt aaatatatat
       61 ggagcaactc gttattcggc aactccatca tcaatatcta tcttcaggat ggagtcgacc
      121 taggtggaag cgaagctgat atcgcggcga tcgcacaaac ctccgtcaag gcgctgccgc
      181 ccgatatcaa tgcgcccatg gtcatgcgcc tgtttccatc tcaagtgccg gtggccacat
      241 tacaagtcac ttcagactca cacacaccgg cggagctcta caacctcagc attatgcgaa
      301 tccggcccct cttggtcacg ataccgggcg cgatcctgcc gcatccctac gggggccagg
      361 acatgcaggt gatgatcaat ctcgatcaac agaagttgct cgcacggcac ctcactccag
      421 cggatatcca ccaagccctc gacaggcaga atctcgtgtt gcccgggggt gatatcaaga
      481 tcaagtcaac cgattggatc gtcttgacca acgcgcagcc actgaagatc gaagatttcg
      541 acgacatccc gatcaagaag gagggcaatg cctggatcca tttgcgcgac gtcagcgtga
      601 cgcgtcttgc cgggcgggtg caaacgaacg ccgtcatagt ggacggacag caggccgtca
      661 tcatcgtcgt gatgaagagc agcgaggcgt ccaccctgga agtggtggat ggaatcaaag
      721 agatcattcc gcgcattcag gaggtcgtgc ctgaaggtgt gaaggtgaag ctcctcatcg
      781 acgcttcggg tttcgtgaag caggcgatcg cagatgtgtt gcacgaaatg gtcatcgccg
      841 ccgggctggt cggcttgatc gtgctggtat tgctcggttc ctggcgaccg acggtcatcg
      901 tcctgacgtc gatcccgttg tccatccttg cctcgctcat cgggctgcat gcgctcgaac
      961 agtcgatcaa tatcatgacc ctgggaggat tggcgctggc cgtcggcatt ctcgtcgaca
     1021 acgcagccgt catgatcgaa aatatcgaca cgcacctcgc cacgggtcgc ccggtggagg
     1081 aagcgattat cgacgccgct aatcagatca ttctcccgac cttcgtcgcc acgctcgcca
     1141 ttacgatcgt ctgggtcccc atgttccatc tgagcgggat ctccggctgg gtgtttccgc
     1201 cgatggcgca ggcggtcatc tttgcgatga tcgcctcctt catcttgaca tacacactgg
     1261 tgcccaccat ggcgaaatat attctgaagg ctcacaacgc gcatgggcat ggtggacaat
     1321 cccacgcgga acagtcgcgg aacatcttca cccgcttcca gcagggattc caggacggtt
     1381 ttgaacggtt ccgtgaacgc tataccatgc agcttgaacg ggccgttgcc catcgtggcc
     1441 gtttcgtcgt cgtctcgtct gccgtagcgg cagcctgtct cgtcctcttt tacttcaacg
     1501 ggcgcgagtt ctttccggag atccggtcaa acatcttgca g
//
LOCUS       DATMTI010000702         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_833782, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000702 DATMTI010000000
VERSION     DATMTI010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833782"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(401..790)
                     /locus_tag="VN039_11285"
     CDS             complement(401..790)
                     /locus_tag="VN039_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46583.1"
                     /translation="MSTPINNLSSGSPYQADALNTYQQRRQSFEALAQALQSNDLNAA
                     QQAYASLVQNTPGGAIPPNSPLAQIGQALQSNDLPSAQNAFATLQAGRHKHHHHHQGN
                     PQPDPSATGSSQTSTTSEASNKIDLAA"
     gene            complement(884..1405)
                     /locus_tag="VN039_11290"
     CDS             complement(884..1405)
                     /locus_tag="VN039_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46584.1"
                     /translation="MSISNINSSPVFQWFQQSLSTAGLTVGNQSSSASNDTQSGTNSI
                     SPFQQALQMLTSQTAQPTDPQQLLNVNGTQGQHHHHHHHHSGGESKDHGNGSFIDQLA
                     QSILSDLQQTDDTGASSATSPSSAPNGSGESFIDGLAGAIANNLPAKYHQATDSSQAP
                     LSTSPPTQVSAVA"
     gene            1865..>2688
                     /locus_tag="VN039_11295"
     CDS             1865..>2688
                     /locus_tag="VN039_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV46585.1"
                     /translation="MSRTTNSPNPIHVLLVDDHPLLRKGLQSLVGSSGEFHIIGEAAD
                     GEQACQLAQGLQPDIILMDIEMPKMNGIDATRWIKTALPHIIVIGLSINQSAVVVNTM
                     KAAGASAYLTKDTAPEDFWHVTRSTLTQHTYNFPGLLARCLVMVALLSMMNCTRLPPI
                     DQKYYSSRVYALPYERVFNAVHARVAEYPMGMGEADQTDGIVKSRIGGTMPGSGATVG
                     YQVTVVVSQVGKQTRVTPDWQMNVSSEPTKTHLVPVSIDERPRLYIEFFEELDKYF"
BASE COUNT          618 a          666 c          759 g          645 t
ORIGIN      
        1 ctcatcggga attgtcgaat ttccgcgcgg atcagctatt tctgcaagga gtccaggaag
       61 gattcgatag ctcatgacgc agttgaaagt ccgacaaacg agtagccgtt gttcatgaaa
      121 tcaccggagg tgttctcttc cgtcgcctgg agccatcgga tagttccagg tcaaactagt
      181 aagatgactg aaggttttta tccgccggcc tcaatttaga aaggtggagc tagctcatgg
      241 aatcatagcg aaggctagga ggagttccat actaggaaaa aacatagggg aagacaatag
      301 agcgcagagc cgcttgcggg cacagtggag ggcaatgcag agacggcaga gccctccatc
      361 ggcccagttc agagcaacct atcaaatgtg cggtgattct ctatgctgca aggtcgattt
      421 tgttactcgc ctccgatgta gtacttgttt gagaagatcc ggttgctgaa ggatcaggct
      481 gcggattccc ctggtgatgg tgatgatgct tgtgtcgtcc tgcctgtaag gtggcgaagg
      541 cattctgagc cgacgggaga tcattcgatt gcagtgcttg gccgatttgt gcgagaggac
      601 tgttcggcgg aatcgcacct ccgggagtgt tctgaacgag cgaggcatag gcctgctgcg
      661 ctgcattgag atcgtttgac tgaagggctt gtgcgagggc ttcaaagctc tgtcgtcgtt
      721 gttggtacgt gttcagtgcg tccgcttgat aaggagaccc ggaactcaga ttgttgatcg
      781 gtgtgctcat cgtgattctc cttcgtgatt gagatgtgac gagtggaatt gcgaatcttt
      841 tctgtccgac attgagcgaa tcctgctcgc ttgagcgctg cctttaagcg acggcactca
      901 cttgggtagg tggactggtc gataagggag cctgactaga atccgtcgcc tggtggtatt
      961 tagcgggtaa gttattggcg atggcaccgg cgagcccatc gatgaatgac tcgccactcc
     1021 catttggagc actggatggg ctagtggccg acgaggctcc cgtatcgtca gtttgttgaa
     1081 gatcgctgag aattgattgc gccagttggt cgataaaaga cccattccca tgatccttgc
     1141 tttctcctcc gctgtgatgg tggtgatgat ggtgatgttg cccttgtgtg ccgttgacgt
     1201 tgagcaattg ttggggatct gtcggctgtg ccgtctgact cgtcagcatc tggagtgctt
     1261 gctgaaacgg cgagatgctg ttcgtgcctg actgagtatc attagacgca ctagaggatt
     1321 gattgccgac tgtgagccca gccgtcgaaa gtgactgttg aaaccattgg aatacgggag
     1381 acgagttgat atttgagatc gacatagagc ctcctcagca aacggcagcg ctcgcaatcc
     1441 gtgcgagccg tcccaccgct ccagaaacaa cagctcgtcc ttgagctgcc gtgacaggag
     1501 cctttcgtgt ttatccagca agcaaagacc gcgcctcgtg gtgcttgttc ccaacacaat
     1561 aataaaatga agaatcgggc gtgtgtcatt ttaactcggc aagagtgaca atcgtcttcc
     1621 atgaactgag tcacctgcac ggcaatttct tccccgatca gaggatgtac cagaacgact
     1681 cttggccgct ggagtcgcaa ggcttcaggg ttttttattg cctcgttgat gctggtgggt
     1741 gccggagcag cgtcaaggat acacctgggg ttttgactag ggcctatccc gtcacaacat
     1801 cacctacaat gccctgcctt atcactcata gcgcctctat gagtatggaa caaattcacg
     1861 tcacatgagc agaacgacaa actcaccaaa ccccatccac gtgctgctgg tggatgacca
     1921 tcctcttctc cgcaaagggt tgcagagctt ggttggctcc tctggggagt tccacatcat
     1981 cggcgaagcg gcagacggag agcaagcctg ccagctggct caggggctac aaccggatat
     2041 catcctcatg gatatcgaaa tgccaaaaat gaatggcatc gacgcgactc ggtggattaa
     2101 aacggcgctc ccccacatca ttgtaatagg gctttcgatc aatcaaagtg ccgtcgtcgt
     2161 gaatacgatg aaagcggcag gagcttcggc ctacctcacc aaagacactg cgccggagga
     2221 cttttggcac gttacccggt ccacgctgac ccagcataca tataactttc ctggattact
     2281 ggccaggtgc ctggtgatgg ttgcgcttct ttccatgatg aactgcacac gattgcctcc
     2341 aatagaccag aaatactact cctcgcgtgt ctatgcattg ccctatgaac gagtgtttaa
     2401 tgccgtgcac gctcgtgtgg ctgagtaccc aatgggcatg ggggaagctg atcagactga
     2461 tgggatcgtc aagagccgca tcggcgggac gatgccaggc agcggcgcga ccgttggcta
     2521 ccaagtcacc gtggtggtat cgcaggtagg gaagcagaca cgagtgaccc ccgactggca
     2581 aatgaacgtt tcatcagaac caacaaagac tcatctggtt ccagtcagta tcgacgagcg
     2641 gccacggctc tatatagaat tcttcgagga actcgacaag tacttcaa
//
LOCUS       DATMTI010000703         4473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_704446, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000703 DATMTI010000000
VERSION     DATMTI010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704446"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VN039_11300"
     CDS             <1..280
                     /locus_tag="VN039_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46586.1"
                     /translation="LVVDILGYGERSQPKDDRDHPQRDPRTEQRREISEPYWEHGRRI
                     GTHGQNCKNSRMGVPPSARFLDGSLGMDFSGMIRYITTPSSSQRERDL"
     gene            296..1357
                     /locus_tag="VN039_11305"
     CDS             296..1357
                     /locus_tag="VN039_11305"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWV46587.1"
                     /translation="MNRPIDNQHVIEIKALPSPRAIKTKLPITDQAAALVVETREAIR
                     RILHGKDRDRLLVIVGPCSIHDPDAAYEYAVKLKPVADTLRDRLLIVMRTYFEKPRTT
                     VGWKGLINDPHLDGTCDIATGMELAREILLKINQLGLPCGTELLDPISPQYIADLISW
                     AAIGARTTESQTHREMASGVSMPVGFKNGTEGSLQVAVNAMISARTLHHFVGINADGQ
                     TAIIRTMGNPDRHIVLRGGGGRTNYEEEHVARTEAAVAGEGIARPIMIDCSHDNSKKD
                     HTRQGLVAHEVLRQFRGGRQTIMGLMLESNLNPGRQAWKEDVALLHGVSITDACLGWD
                     ETERLLTELAAAITPKPVS"
     gene            complement(1826..3778)
                     /locus_tag="VN039_11310"
     CDS             complement(1826..3778)
                     /locus_tag="VN039_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c/FTR1 family iron permease"
                     /protein_id="HWV46588.1"
                     /translation="MKATRTFLFLPKFFPWWLTLVLLATNLHGVAMAAPADDEKAQTI
                     VHMLDYVGVDYPDFVQDGTVINPEEYAEQREFATQAMLLLGTLSTVPEQPMLLQQAHE
                     LLSRIEAKAPGDEVSMLAGQLRVGVIQAWQLSVAPRQPPALQKAEKLFAQHCTLCHGT
                     QGRGDGPLAKGMEPTPRDLHDEVRMRQRSLYGLYNTISLGVRGTPMRAFTELSEADRW
                     ALAFFVGNLRADPEMVAKGETLWRQGEGKAAFESLRALVTMAPSEQAPPGSAIDAVRA
                     YLTQQPQALQTQVPAQGPLVFSRTKLDETAQAYAQGNREDAQRLAIAAYLEGFELVES
                     ALDNVDALLRADTEREMMALRAAIDEGRPSEAVTAQAVKVTALLDRAAAVLSGSSLSP
                     NTAFVSSTLILLREGLEAILVVAAIVAFTVKTGRHDATPYIHAGWIGAVALGGITWVV
                     ARYALNISGANRELTEGITALLAAAMLLYVGWWLHNRSNAQAWNRFIREQINTALGKR
                     TLWAMAGISFLVVYRELFEVILFYETLWSQVGAGGQGAVLWGIAAAALLLMLIGGMIL
                     RYSVRLPIGPFFAVTSILLALMAVVFVGNGVAALQEAGVLETTTVRFFSFPLLGIHPT
                     AQSLVPQALTLVLIAGGLWSTRAKSS"
BASE COUNT          983 a         1330 c         1273 g          887 t
ORIGIN      
        1 actcgtggtg gacatcctgg gctacggcga acggagccag cccaaggatg atagagatca
       61 tccccaacgc gacccccgaa cggagcaacg aagagagata tctgaacctt attgggaaca
      121 tggtcgtaga ataggcaccc atggtcagaa ttgcaagaac agccggatgg gcgtgccccc
      181 atcagctcga ttcctggacg ggtcactcgg tatggacttt tccggcatga ttcgttatat
      241 tactaccccc tcaagttctc aacgagaacg agatctctga aggacatacc ccttcatgaa
      301 taggccgatc gacaatcagc atgttattga aatcaaggcg ttgccgtctc cgcgcgccat
      361 taagaccaaa ctgcctatta ccgatcaggc tgcggcactc gtcgtcgaaa cccgagaagc
      421 gattcgccga attctccatg gaaaggatcg tgacaggctt ctggtcatcg tgggaccgtg
      481 ctccatccat gatcccgatg ccgcctatga gtatgccgtc aagttgaagc cggttgccga
      541 taccctgcgt gaccgtctcc tgatcgtgat gcggacgtac tttgaaaaac cacgaaccac
      601 cgtcggatgg aagggtctga ttaatgaccc gcatctcgac ggtacgtgcg atatcgcaac
      661 aggaatggag ttagccaggg agattctcct caagatcaat caattgggtc ttccttgcgg
      721 aacggagctg ctggatccga tcagtccgca gtacatcgcc gacctcatca gttgggcggc
      781 gatcggggct cgtaccacgg aaagccaaac ccatcgagaa atggcgagcg gcgtatccat
      841 gccggtcggc ttcaagaacg gcactgaggg cagcttgcag gttgccgtca atgccatgat
      901 ttccgcccgc acattacacc attttgtcgg catcaacgcc gacggccaaa ctgcgatcat
      961 cagaaccatg ggcaaccccg accgccatat cgttctccgt ggcggagggg gaaggacgaa
     1021 ctacgaggag gaacatgtcg ctcggactga agccgcggtc gccggagaag gcattgcccg
     1081 tccgatcatg atcgattgtt cgcacgataa ttccaagaaa gatcacacac gccaaggctt
     1141 ggtcgcccat gaggttctgc ggcagttccg tggaggtcgc caaaccatca tgggtctcat
     1201 gctcgaaagc aacctcaatc ccggcagaca ggcatggaaa gaagatgtag ccctccttca
     1261 tggagtctca attaccgatg cctgtctggg ctgggacgag accgaacgtc tgttgaccga
     1321 acttgccgca gcaattactc cgaagccggt ttcctgaact ctattctttc cgaccattgt
     1381 agctcggagc accaggccgt ttgtgcggaa ccccacgtcc cggacgccac agtcaccgct
     1441 gagacgatca tacaaatttc gaaagcctcg atcgcggcgc ttttcgtcat gacaaaggtc
     1501 gtgaagaagt tgaacctcgt tccactcgat tcgctgcagc ttggaaaccc ccgcagaggt
     1561 cgtcgatcca ccccgaccct tgcttgtaac gcttccgacg aacggccttg atcaaccacg
     1621 cccgccgagc cagaacaaga ggacgccgac acttggaggt tcatagagcg agtgacggag
     1681 tgcctccatc atgagcatcg caccgcgact tctgcgccgg gctggaacgg tacatcgaga
     1741 agtatcagat tcaaagcgtc acagtcgaag acgacctccc ccggagacag tatatgaggc
     1801 ggtagatgca gcgcgctcgt ttcgtttatg acgacttcgc ccgggtggac catagcccac
     1861 cggcgatcaa cacaagcgtc agcgcctgcg gcacgaggct ctgcgcggtt gggtggatgc
     1921 ccagcagcgg gaatgagaag aagcgcaccg tcgtcgtctc cagcacaccc gcttcttgca
     1981 gcgccgcaac accgttaccg acgaagacga cggccatcag cgccagcagg attgaggtga
     2041 cggcgaagaa cggcccgatc ggcagacgca cactgtagcg aaggatcatc cccccaatca
     2101 gcatcagcag cagtgctgcc gcggctatgc cccacagcac tgcaccctgc ccacccgcgc
     2161 cgacctgcga ccacaatgtc tcgtagaaca ggatcacttc gaacaattcc cggtaaacga
     2221 cgagaaacga gataccggcc atcgcccaca gcgtgcgctt gcccagcgcg gtattgattt
     2281 gttcgcggat gaagcgattc caggcctgcg cgtttgaacg gttatgcaac caccagccga
     2341 catagagcag cattgccgcg gccagtagtg cggtgatgcc ttccgtcagt tcgcggttgg
     2401 cgcccgagat gttcagcgca taacgggcta cgacccatgt gatgccgccc aacgccacgg
     2461 ctccgatcca accggcgtgg atgtaaggcg tagcatcatg ccgcccagtc ttgacggtga
     2521 acgctacgat ggctgcaacc accaagattg cctccaatcc ctcccgtagc aagatcagcg
     2581 tagaactcac gaaggccgta ttcggcgata ggctgctgcc ggagagcaca gcagcggcac
     2641 gatcgagtag tgccgtgacc ttcaccgcct gtgctgtcac ggcctcggac ggccgaccct
     2701 cgtcgatggc cgcgcggagc gccatcatct cgcgctcggt gtccgcacgg agcagcgcat
     2761 caacgttgtc cagtgccgat tcgaccagct cgaagccttc aaggtaggcc gcgatcgcaa
     2821 gacgctgcgc atcttctcgg ttaccctgcg cgtaggcctg ggccgtctca tcgagcttgg
     2881 tgcgggagaa gaccagcggc ccctgggctg ggacctgggt ctgcagtgcc tgtggctgtt
     2941 gcgtcaggta ggcacgcaca gcgtctatcg ccgagccggg aggggcttgc tcacttggcg
     3001 ccattgtcac cagcgcacgc aagctttcga acgctgcttt gccctcgcct tgtcgccata
     3061 atgtttcgcc tttcgccacc atttcaggat cggcgcgcag gttgccgacg aagaaggcca
     3121 gcgcccagcg atcggcctct gaaagttcgg taaaggcgcg catcggcgtg ccgcgcacgc
     3181 caagtgagat ggtattgtac aggccgtaca ggctgcgctg gcgcatgcgg acctcatcgt
     3241 gtaaatcacg aggtgtcggt tccataccct tggcaagcgg cccgtcgccg cgaccctgtg
     3301 ttccgtgaca cagggtgcag tgttgcgcaa acagcttttc ggccttctgg agggctggag
     3361 gttgccgtgg tgccacgctc agctgccacg cttggatcac gccgacacgc agctggccgg
     3421 cgagcatgga aacctcatca cccggcgcct tggcctcgat acgcgagagc agctcatgag
     3481 cctgctgtag cagcataggc tgctccggca cggtggataa tgtgccgaga aggagcatag
     3541 cctgggtggc gaactcgcgc tgttccgcgt attcctcggg gttgatcacc gtaccatcct
     3601 gcacgaagtc tgggtagtcc acaccgacat aatcgagcat atgaacgatg gtctgcgctt
     3661 tttcgtcgtc agccggtgcc gccatcgcaa cgccgtgcag gttcgtggcc aacagcacta
     3721 gcgtcagcca ccacgggaag aacttcggta agaacaagaa ggtacgtgtt gctttcatcg
     3781 catctcaaca ttctgtgtac ttggtgctct ctcgttcatg gtctgctcta ccccattgtg
     3841 ttggaccgtg ccgccgcttc tcagccgcct gcggcggttc atgctacggt ctcagctata
     3901 ttacgggtcg taccacagtg tgctggaaag catgtgtcaa ttacagattg atgcgaagac
     3961 tagtgtcgaa ccagcgatgc caggcttttc aacatggatg tccatccggt cagccccatg
     4021 agcaaaatcg tgggcggtat cgcacgtgta tgagacacca ttgacatgct catctcttca
     4081 cggagggaca agggcgaatg acgcgcacat tttggcaagt gcctgtgcga caagtcaatg
     4141 aggacctcaa agtccccgac gagacgtgat gctcaaatcc gacgggacgc attcaatctt
     4201 caatgacaat ggactcgcgg tggaaaactc ttgacagcgg acggtagcgc gatgagcgca
     4261 cttccatggt gcgtgaaatt taggacgtcc ggttttacga aagcctccac attggagcat
     4321 gaccatactg gaccgaggga ccggataaga tctacgagag ttcgagatga taccggtcca
     4381 gcgtcgtggc tttatggcgt gcgaggttgg agagtgcctt gttgtagtca atgatggccc
     4441 gcaactcgtt tccctgcgcg gtcgcaagat cac
//
LOCUS       DATMTI010000704         1564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_704490, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000704 DATMTI010000000
VERSION     DATMTI010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704490"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1564
                     /locus_tag="VN039_11315"
     CDS             <1..>1564
                     /locus_tag="VN039_11315"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWV46589.1"
                     /translation="PYGRDKAKVSLQVLDRLTTAPMGRYVLVTAINPTPLGEGKTTTS
                     IGLAMGLSRLGHRTALTLRQPSLGPVFGMKGGGTGGGHAQVAPMEDINLHLTGDAHAV
                     AVSHNLLSAFLDNHLFHGNTLSIDPQRTTWPRTLSISDRALREVLLGEKPDRRPGQFV
                     ITEASEVMAALALARDQQDLRERLGRILVGLTKAGTPVTAEELRCVGSMAVLLKDALM
                     PNLVQTLEGTPAFVHTGPFGNIAHGNCSVISDAIALRCADFVITEAGFGSDLGAEKFF
                     NIKCRASGFAPTVAVVVATLRALKLHGGGGVAKAGVALPSSLTGPNREALSKGIANLE
                     QHVANVLAHGVPVVVAVNAFKDDPQDELEWVREQSLKMGAADAAVSTHWADGGKGAEE
                     LAVAVIKASERPAHFNHLYEVSWPIRKKIETIATRMYGADGVTFEPEAERQIETAEAL
                     GYGGVPVCMAKTPLSLSHDPGLKGRPTGFTVPIRELRILAGAGFVTAVCSGIQLMPGL
                     PKKPAGERINLDP"
BASE COUNT          336 a          429 c          485 g          314 t
ORIGIN      
        1 ccccttatgg gcgggacaag gccaaggtct cgctacaagt actggatcgc ctgacaactg
       61 ccccgatggg gcggtatgtc ctcgtcacgg caattaaccc cacacccttg ggagagggaa
      121 agaccacgac atcgataggc cttgccatgg gactctcgcg gctagggcat cgaaccgcgc
      181 ttaccctcag acagccatcg ctcggtccgg tgtttggaat gaagggagga gggaccggtg
      241 gggggcatgc ccaagtcgct ccgatggaag atatcaacct tcacctgacc ggggatgccc
      301 acgcagtggc tgtcagccac aatctgctct cggcgttcct cgataatcat ctgttccatg
      361 gcaatactct ctcgatcgat cctcaacgga ctacgtggcc tcggaccttg agtatcagtg
      421 atcgtgctct ccgggaggtc ctgttgggag aaaaaccgga cagacgtcca gggcaattcg
      481 tcatcaccga ggcttcagaa gtgatggcgg cactggcatt agcgagggat cagcaggatc
      541 tccgcgagcg gctcggccga atactcgttg gactgacgaa ggccggtacg ccggtcacgg
      601 ccgaagaact tcgttgcgtg ggatcgatgg ctgttctcct aaaggacgcc ctcatgccga
      661 atctcgttca aactcttgaa gggactccgg cattcgtcca tacggggccc ttcggcaaca
      721 tcgcgcatgg caactgttcg gtcatttccg atgcgattgc gctgagatgt gctgacttcg
      781 tcataacaga ggcgggcttc ggcagcgatc ttggagcgga aaagtttttt aacatcaagt
      841 gtcgtgcatc gggatttgcg ccgaccgtgg cggtggtcgt ggcgacatta cgggcgctga
      901 aactccatgg cggcggaggc gttgccaagg caggcgttgc cttgccttcg agtctgacgg
      961 gtccgaatcg ggaagctctc tcgaagggaa tcgccaatct ggagcagcat gtcgccaatg
     1021 tcctcgcaca cggggtaccg gtcgttgtcg ccgtcaatgc cttcaaagat gaccctcagg
     1081 acgaattgga gtgggttcgg gaacagtcgc tcaagatggg ggcggcagac gcagcggttt
     1141 caacccactg ggccgacgga gggaaggggg ctgaagagct ggcagtcgcc gtcatcaagg
     1201 catctgaaag gccggctcac ttcaatcatc tgtacgaagt gtcttggccg atcaggaaaa
     1261 agatcgaaac cattgcgacc cgcatgtacg gcgcagacgg agttacattt gaaccggagg
     1321 ccgaacgtca aattgaaaca gcagaggcat tggggtacgg cggcgtaccc gtctgtatgg
     1381 caaaaacccc tttatcgctg tcacacgatc ctgggctgaa gggacgaccg accgggttta
     1441 cggtgccgat ccgagagttg cggattctag ccggagcggg atttgtgacc gccgtctgtt
     1501 caggcattca gctcatgccg ggattgccca agaagccagc aggtgagcgg atcaatctcg
     1561 accc
//
LOCUS       DATMTI010000705         4002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_26721, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000705 DATMTI010000000
VERSION     DATMTI010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26721"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VN039_11320"
     CDS             <1..877
                     /locus_tag="VN039_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWV46590.1"
                     /translation="LAGDHIYKMNYAEMFHWLIAKSADAIVGAIDIPVQEANRFGVIA
                     VDEDYRITRFDEKPANPIPLPNDPAHAFGSMGIYLFRTKAIREHLIADAREDSAHDFG
                     KNIIPRMIERGRVYAFKFQDANKKAVQYWRDIGTLDAYWEANMDLVAVDPQFNLYDSE
                     WPIRTYQGQFPPAKFVFAQDFQGGRMGVALDSIVCGGCIVSGGRVQNSVLSPNVRVQD
                     HADVRESIIMENVTIGAQSRIRRAIIDKDVMIPPHTEIGYDREADAQRFTLTESGLVV
                     ISKGMKLHAAVDPSG"
     gene            852..2711
                     /locus_tag="VN039_11325"
     CDS             852..2711
                     /locus_tag="VN039_11325"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWV46591.1"
                     /translation="MPPSIHPVDLITALRHKHLTPAIVFLTSRRACDEAMETFDHADL
                     VLPPARQEAIAVALERITIQHPSIAEHPLIPIVRRIGVAAHHAGHLPSWKIAIEELMR
                     QGHLDAVFATTTLAAGVDFPARTVVITQSSIRKSRDFTDLTIGEVQQIAGRAGRRGKD
                     HVGFAIVTPSPYIDLAVLTKGLTGQPEAINSQFTISYPMVLNLLKAHPHEHIQGILAK
                     SFAQFQLNQRAELLEQKLDAVHVQMEPFGPRVCTDWITQWHTFDHARRHRHTRHQTHR
                     SEPPEISARLPFLTPGRVVGLGRGRGIVLRQYRSKGQKNSMLTVLRPDSAVTECPVTG
                     VKEVYDRTYDCAETPTYPWCSTETFDRLSHQLEDLPSHVPVLPILISPGIEPLPDAIV
                     QSLGDFPCPTCPSRPACQKDFITASRLRQEQQRHTKSIQALRTSLWHRFQERVEVLQQ
                     FGYLTTATQLTIDGEWARLIRIDHSLLITELIRAEAFTGADPSLLAGVLASLAHDDDR
                     PGAFPRISPGLSSLLGQVRKLAESVSPYEDPPLLRADVAALVERWVADPTLTWIGLCR
                     LTTMAEGDIYRLLARTLEFLSQVQALKSTHPGLAESASQAIILIRRGVLEELP"
     gene            2782..3837
                     /locus_tag="VN039_11330"
     CDS             2782..3837
                     /locus_tag="VN039_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWV46592.1"
                     /translation="MTTEELEKLLAQQPVALLHRLARGRVHRHFRAGKRRLIELLLQH
                     SSQNRAGLESDLQVLIEEQYPRSEAGSSRIHHPPVIAHKKASPRGAGPESAEAAISLS
                     AWLEGLGVPSPQPFVPDPWQSEALSALNETDVVISVPTGSGKTYVAVEAARRAMDDNR
                     TVIYTSPLKALSNTKYTEFSRIFGPDKVGILTGDRQENGQAPLLIMTTEILRNLLYDA
                     ASGEIDIRLDTLGLVILDESQYLADPERGVVWEETIIFCPSQARLLLLSASIGNPQDI
                     ADWLTSIRSTVCRLVRHGKRTVPLRAGYLHPNGKLTPLFRTLGIPYGHPSHLHPEAKR
                     LFLEYEEETLPGGRPRR"
BASE COUNT          884 a         1229 c         1066 g          823 t
ORIGIN      
        1 tctcgccgga gatcacattt acaagatgaa ctatgcggag atgttccatt ggctcatcgc
       61 caagagcgcg gatgcgatcg tcggcgccat cgacattccc gtccaggagg cgaatcgctt
      121 cggagtcatc gcagtggacg aagactaccg gattacccgc ttcgatgaaa agccggcgaa
      181 tcccattcct ctccccaacg atcccgccca cgcctttggg tcgatgggca tctacctgtt
      241 ccgcacaaag gcgatccggg aacatctgat cgccgacgcg cgagaggaca gcgcgcatga
      301 tttcggaaaa aatatcattc ccagaatgat cgaacgaggt cgtgtctatg ccttcaaatt
      361 tcaagatgcc aacaaaaaag ccgtccagta ctggcgcgac attgggacgc tcgacgctta
      421 ctgggaagcg aacatggatt tggtcgccgt cgatcctcaa ttcaacctgt acgactctga
      481 atggccgatc cgaacctatc agggacaatt tccacccgcc aaattcgttt tcgcacagga
      541 ttttcaggga ggacggatgg gcgtcgccct cgattcgatc gtctgcggcg gctgcatcgt
      601 ttcgggcgga cgggtccaaa attcggtttt gtccccgaac gtccgcgtgc aagaccatgc
      661 ggatgtccgt gaatccatta tcatggaaaa cgtgaccatc ggtgcgcaga gccgcatcag
      721 acgcgccatc atcgacaagg atgtgatgat ccctcctcat acggaaatcg ggtacgatcg
      781 agaggccgac gcgcaacgat ttactctcac tgaatcaggc ctcgtggtca tctcaaaggg
      841 aatgaaactg catgccgccg tcgatccatc cggttgatct catcactgcg ctccgtcaca
      901 agcatctcac gccggccatc gtgtttctca cctcacgccg ggcctgtgac gaggccatgg
      961 agacctttga ccatgccgat ctcgtactcc ctccggcacg acaggaggcg attgcggtcg
     1021 cgcttgagcg gatcacgatt caacacccca gcattgccga gcacccgttg attcccattg
     1081 tgaggaggat cggggttgcc gctcatcatg ccgggcatct gccttcttgg aaaatcgcga
     1141 tcgaggagct gatgcggcaa ggccatctcg atgcggtatt cgccacgacg accttggcgg
     1201 cgggtgtcga ttttcccgcc cgcacggtcg tgattaccca atcgagcatc cgcaagtccc
     1261 gtgacttcac cgacctgacc atcggcgaag tccagcaaat cgccggacga gccggccgcc
     1321 gcggaaaaga tcatgtgggg tttgcgatcg tgactccctc tccttatatc gatctcgcgg
     1381 ttctgaccaa agggttgacg ggacagccgg aagccatcaa cagccagttt accatcagct
     1441 acccgatggt cttgaacctc ttgaaggccc atccccatga gcacattcaa ggaatcttgg
     1501 ccaaaagttt cgctcaattc caactgaatc agcgcgcaga actcctcgaa caaaaactag
     1561 acgcggtcca tgtgcagatg gaaccgttcg gtcctcgcgt ctgtacggac tggattaccc
     1621 aatggcatac cttcgaccat gcgcgaagac accgccacac gcgacatcag acgcaccgat
     1681 cagaaccgcc ggagatttcg gcccggttgc cgttcctgac gccggggcgt gtcgtagggc
     1741 tcggcagagg ccgcgggatt gtactccggc aatatcggag caagggccaa aagaattcca
     1801 tgctcaccgt cttacgaccg gacagcgccg tcaccgaatg ccctgtcacc ggcgtgaaag
     1861 aagtctacga tcgcacgtac gattgcgcgg agacgccgac atatccttgg tgttctaccg
     1921 aaacgttcga ccgactcagc catcaattgg aagatcttcc gtcgcatgtg cccgtgcttc
     1981 ccatactcat atctccgggc atcgagccgt tgcccgatgc catcgtccaa tcgttaggtg
     2041 acttcccctg cccgacttgt ccttcgcgac cagcctgcca gaaagacttc ataaccgcat
     2101 cgcgccttcg ccaggaacaa cagcgccata cgaaatccat acaagcgcta cggaccagtt
     2161 tgtggcaccg ctttcaagag cgcgtggaag tgctgcagca attcgggtat ctcaccacgg
     2221 cgacacagtt gacgatcgat ggagaatggg caaggctgat ccgcatcgac cattcgctgc
     2281 tgatcaccga actgatccgc gccgaagcct ttaccggagc agacccgtcc ctcctcgccg
     2341 gcgtcttggc cagtctagcc cacgacgatg atcgacccgg cgcgtttcca cgcatcagtc
     2401 cggggctgag ttccttgctc gggcaggttc gcaaattggc ggagagtgtg tctccttacg
     2461 aagatcctcc cctcctacgc gccgatgtgg cggcgttggt tgagcgctgg gtcgccgacc
     2521 cgactctcac atggatcgga ctctgtcgat tgaccacgat ggcggaaggc gacatctacc
     2581 gattattggc taggaccttg gagtttctgt cacaggtgca ggccttgaaa tctacacatc
     2641 ccggcctcgc tgaatccgcc tcacaagcaa tcatattgat ccggcgggga gtgttggagg
     2701 aactgccatg agccgtgtgg gactgagggc cgaggcttca ggactgagct atcctgcgac
     2761 cagtcctcag cacttccaat tatgaccacc gaagaacttg aaaaacttct cgcacagcag
     2821 cccgtggcgc tcttgcatcg cctggcccga ggacgagtcc atcggcactt tcgtgccggc
     2881 aagagacgac tgatcgagct gcttcttcag cattcaagcc agaatcgtgc cggtctcgaa
     2941 tccgatctcc aggtcttgat cgaggagcag tacccccgat cggaagccgg atcgtcccgc
     3001 atccatcatc cgccggtgat cgctcacaag aaagcgtcgc cgcgtggagc agggccggaa
     3061 tctgccgaag ccgccatctc actatcggcc tggctggaag gccttggggt tccttctccg
     3121 caaccatttg tcccggatcc ctggcaaagc gaggccttgt ccgccctgaa tgagaccgac
     3181 gtggtcatca gcgttcccac cggcagcgga aagacatatg tcgccgttga agcggcccgc
     3241 cgggccatgg acgacaatcg cacggtcatt tacacgtcac cgctcaaggc cttgtccaac
     3301 acgaaatata cggagttttc tcgaatcttc ggtcctgata aagtcggcat tctcaccgga
     3361 gatcgccagg agaacgggca agcaccgctg ctcatcatga ccacggaaat tctgcgcaac
     3421 ctgctctatg atgccgcaag cggggagatc gatatcagat tggatacgtt gggactggtg
     3481 attttggacg aatcgcagta tctcgccgat cccgagcgag gagtggtgtg ggaagaaacc
     3541 atcatctttt gtccttcaca ggccagattg ctgttacttt ctgcctcaat cggcaatcca
     3601 caggacatcg ccgattggct gacatccatc cgatccactg tctgtcgact cgtacgtcac
     3661 ggcaaacgca ccgtgcccct cagagccggt tatctgcatc ccaacgggaa attgacgccg
     3721 ctgtttagaa cgttggggat cccctacggc caccccagcc acctccatcc agaagccaag
     3781 cgcctctttc ttgagtatga agaagaaacc ctacctggcg gacgtccaag acgatagctg
     3841 acacgcgatc tcaagaagtt aggatctaga atgacgccgt cagggtgacg gtcacaccat
     3901 ggcggatcgt ttgaagttcc gtgagcggag ctgtctctga gcccgtccca agcccatgaa
     3961 cttcccaccg accgtagtaa gtccggttcc acataacccg at
//
LOCUS       DATMTI010000706         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_620482, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000706 DATMTI010000000
VERSION     DATMTI010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620482"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..536
                     /locus_tag="VN039_11335"
     CDS             165..536
                     /locus_tag="VN039_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46593.1"
                     /translation="MTIKVLMKALQADCVDQCNSYQMQEVIGCSKGGLIIKIHTAVDA
                     LGNPVRFMLSPGQGPDCTQALLLLRRVHHTGRDCRQGYDSDEIVNWIASRDAQGRHPI
                     TLNPQDAKELRRRSLGGTTPY"
     gene            956..1038
                     /locus_tag="VN039_11340"
     tRNA            956..1038
                     /locus_tag="VN039_11340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:990..992,aa:Leu,seq:tag)
     gene            1076..>1583
                     /locus_tag="VN039_11345"
     CDS             1076..>1583
                     /locus_tag="VN039_11345"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HWV46594.1"
                     /translation="MKMEVTELGPMKRALKIEVPADEVTQQFSRAYLELNRQVQIPGF
                     RPGKAPLAILEKRYAKTVEEDVIRKLVPDFYGRAIKQAGISPVVVDIPPLDRVKIKRD
                     SPFTFTATVEIKPTIELRDYKSPNPISLQADKRTVAEEQVDRALEVLREQQARLDAAP
                     PGSALAEGD"
BASE COUNT          422 a          397 c          400 g          364 t
ORIGIN      
        1 actgcaccta aataataagc tctgtatatg tcaaattaag cgttattaag taccaatgat
       61 tcaattctgt tcggaagacg aatgacatga taggtatttg aggtcaaccc ttaaacttta
      121 accgctttat attatggtaa taccgcattg aaggcactgt gcccttgacg attaaggtac
      181 tgatgaaagc gctgcaggcc gattgcgttg atcaatgcaa ttcctatcag atgcaagagg
      241 tgatcggctg cagcaaaggc ggtctgatca tcaagatcca tacggcagtc gatgcgttgg
      301 gcaatccagt gcgtttcatg ctgtcgccgg ggcaaggccc ggattgtacg caggccctgc
      361 tcttgctgag aagagttcac cacacaggtc gtgattgccg acaaggctat gactctgacg
      421 aaatcgtgaa ctggatcgct tcccgcgacg cacagggtcg tcatcccatc acgctcaatc
      481 cgcaagacgc caaggaatta cggagacgct ctctaggcgg aacgacaccg tattgaacgt
      541 tatttcaata ggctcaaacg ctatcgcgga attgccacaa gatactgcaa acgcgccaga
      601 aacttcgcat ccttcatcta tctcgcagcc tcggtgcacc cggtgcaatg gttgctgtaa
      661 ttggcaacag gacagatcct gctgtcgaag ccggcaaatt tctttaccaa gaaagttatc
      721 ggaggtgtac cacggcgaac gtatgggtga agttttttga tgagcaacga ggatggacga
      781 gatcagcaaa cagtctgact ggaccattca tactagaata gggcaccctc ggtcgtatgg
      841 gttgacatta caggtgtcca acgatacaac gctatacgac agcgctgttt tttgcagtct
      901 cggtcactct tctcataagt tagagtgaca gctccgcagc attcttaaca accatgcgag
      961 agtggcggaa ctggcagacg cgcgagactt aggatctcgt gggcaaccgt gggggttcaa
     1021 gtcccccctc tcgcaccact agtccgattc cttggcgagg agtccaatct cgacgatgaa
     1081 aatggaagtg accgagttag gaccgatgaa acgcgccttg aaaatcgagg taccggccga
     1141 tgaggtgaca caacaatttt cacgagcgta tttggaactc aaccgtcagg ttcagattcc
     1201 agggtttcga ccgggaaaag cccctttggc tattttagaa aaacgctatg ctaaaacagt
     1261 cgaagaagac gtcattcgaa agctcgttcc tgatttctat ggtcgagcca tcaaacaggc
     1321 cgggatcagt ccagtagtgg tggatattcc ccctctggat cgggtcaaaa tcaaaagaga
     1381 ctcaccattt acttttacgg cgacagtcga aatcaaaccc acgatcgaac tacgcgacta
     1441 taagtccccc aatcccattt ctctccaggc cgacaagcgt acagttgcgg aggaacaggt
     1501 agatcgtgcc ttggaggtat tgcgtgaaca acaggcccgc ttggatgcgg ccccacccgg
     1561 ttcggcctta gccgaaggag act
//
LOCUS       DATMTI010000707         1882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_620486, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000707 DATMTI010000000
VERSION     DATMTI010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620486"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VN039_11350"
     CDS             <1..758
                     /locus_tag="VN039_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWV46595.1"
                     /translation="RTVGNDSTFYRPPTANQLRHYLNQIPEDFQMCFKVWEEITIPSF
                     SRQARYGLKAGQPNLRFLDAKLFNELVLTPYREAKFEPHMGPLLFEFQQHGMSAGEFC
                     SRLDQFFGQLPNDFRYAVEIRNAGLLGLDYRKVLESHGVAHVYNHWSYMPPLLHQHKR
                     MEERFTAPFTVIRLLTPLNMPYEVAKKRAAPYNKIVEALPQMRKETVTLIEQAIGENR
                     RVYVLVNNRSEGNAPLTVQAFASYLRHEEERYR"
     gene            complement(764..1438)
                     /gene="purN"
                     /locus_tag="VN039_11355"
     CDS             complement(764..1438)
                     /gene="purN"
                     /locus_tag="VN039_11355"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWV46596.1"
                     /translation="MSTSRTTPLRVAVLASGRGSNLQAIIDAIEAGQVQARIVAVISN
                     KKDAVALERARKHGLKDLFVDPKPFTGRSDSREVYDRSLLKTLQQHDAELVLLAGYMK
                     IVTAVLVNAYANRMMNIHPSLLPSFPGLDVQKKAIDWGCKLAGCTVHFVTEGVDEGPI
                     IVQAAVPILDDDTPETLAARILLQEHKIYPRAVQLFAEGRLRVDGRRVLIEDAKPGGE
                     AIISPE"
     gene            complement(1428..>1882)
                     /locus_tag="VN039_11360"
     CDS             complement(1428..>1882)
                     /locus_tag="VN039_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWV46597.1"
                     /translation="VLFEQAKLTVASRLPELDGPIGEVLLTPTRIYAKQILMLVEQFP
                     IKGIAHITGGGITENLPRVFPKGVRARITRTAWSVPPIFDVMSRLGRIDREEMYRVFN
                     MGIGLILVVPPDSVSGVLARAIALGDRGWQIGEIVASTGEEPEVEYVD"
BASE COUNT          441 a          555 c          470 g          416 t
ORIGIN      
        1 ttcgcacagt cgggaatgat tcaaccttct atcgtccacc gactgcaaat cagcttcggc
       61 attacttaaa ccagattcca gaagacttcc agatgtgttt taaggtctgg gaggaaatca
      121 ctattccaag cttctccaga caagctcgct acggcctcaa ggccgggcaa ccaaatcttc
      181 gctttctcga tgcgaagctg ttcaacgaac tggtcctgac gccataccga gaggccaagt
      241 ttgaaccaca catgggccct ctgctctttg aattccaaca acacgggatg tccgcgggag
      301 agttttgttc acgactcgat cagttcttcg gtcagctacc aaatgatttc cggtatgcgg
      361 tcgagatccg caatgcaggc ttgctcgggc tggactatcg gaaagtttta gagagccatg
      421 gtgtcgcaca cgtctacaat cactggtcct acatgccgcc gttgctgcac cagcacaaac
      481 ggatggaaga acgtttcacg gcgccgttca ccgtgatccg cctactcaca ccgctcaaca
      541 tgccgtacga agtcgcgaaa aagcgagccg cgccttataa caagatcgta gaagcattac
      601 cccaaatgcg gaaagagacc gtgaccctca tcgagcaggc catcggcgaa aaccggcgag
      661 tctatgtgtt ggtgaacaat cgctcggaag ggaatgcacc tttgacggtg caagcattcg
      721 caagttacct acgccatgaa gaagaacgat acaggtgagt tcattattcc ggactgatga
      781 tcgcctcgcc gcccggcttt gcatcctcaa tcaatacccg ccggccatcc acccgtaatc
      841 ggccttcggc aaagagttgc acagcgcggg gatagatttt gtgctcttgc agaaggattc
      901 tggcggcaag tgtttcgggt gtgtcatcgt cgaggatcgg cacggccgct tgtacgatga
      961 tcgggccttc atccactcct tctgtgacga aatgcacggt gcagcccgcc agcttacaac
     1021 cccaatcaat ggccttcttc tgtacgtcca atccaggaaa tgacggcagc agcgaaggat
     1081 gaatgttcat catgcgattg gcataagcat tgaccagcac cgctgtgacg atcttcatgt
     1141 aaccggctag caagaccagt tcggcatcat gctgctggag cgtttttaac agtgaccggt
     1201 cgtacacttc acgactgtcc gatcgtccgg taaacggctt cggatcgacg aacaaatcct
     1261 tgagcccgtg cttccgagcc cgctccaatg cgaccgcgtc cttcttattg ctgatgacag
     1321 ccacaatccg agcctgcact tgccccgctt cgatcgcatc gatgatggct tgcaggttgg
     1381 aaccccgacc ggatgccagt acggccaccc tcaatggggt cgttcggcta gtcgacatac
     1441 tccacctccg gctcctcccc ggtggaagca acgatttcgc cgatctgcca gcctcggtcg
     1501 ccaagcgcta tggcgcgagc cagcactccg gacactgaat caggaggtac cacgagaatc
     1561 aacccaatgc ccatgttgaa cactcggtac atttcttcac gatcgattcg tcctaaacga
     1621 ctcatcacgt caaagatcgg cggcactgac caggcagtgc gggtgatcct cgcccgtaca
     1681 cccttcggga acacccgcgg caaattctcc gtaatgcctc ctccggtaat gtgtgcgatg
     1741 cccttgattg gaaattgctc gacaagcatc aaaatctgct ttgcataaat tctggtgggt
     1801 gtcaggagta cctcaccgat aggcccgtca agttcaggaa gacggctggc caccgtcagc
     1861 ttggcttgtt caaacagcac tc
//
LOCUS       DATMTI010000708         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_620546, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000708 DATMTI010000000
VERSION     DATMTI010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620546"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VN039_11365"
     CDS             <1..282
                     /locus_tag="VN039_11365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWV46598.1"
                     /translation="HGMGGPGYKVKAEFNSTPHKRGVVSMARANDPDSAGSQFFICVA
                     DANFLDWQYTVFGEVVSGMEVADKIVHMKRDGRDNPLERAEMTVAISEG"
     gene            299..1009
                     /locus_tag="VN039_11370"
     CDS             299..1009
                     /locus_tag="VN039_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46599.1"
                     /translation="MSNMPLNLHCLILTLTSAFLLFGCAAPHIPSRIIYEDPVNFVRL
                     EEDPEVLAEWPPSHHSHPFTIEPEKLRTILSGLKVQEHWIALQRWMRGESPLVPAFTD
                     EELALLSVRIAEALAEAKDNERVTFYLSEPQTFARRIITTGGLYVHGTQLHILLGNWR
                     IIYGIPAYGMIYDRRYPMRPTAAKGFDLTFQPSEAVIPMKSSLLDGVLANAKDELIID
                     LTKLDSPDPEITRRVLCD"
     gene            complement(1092..>2002)
                     /locus_tag="VN039_11375"
     CDS             complement(1092..>2002)
                     /locus_tag="VN039_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWV46600.1"
                     /translation="NGILPLKELGPDEAYAVPDFELAEELIARGADSARIAISRHDAE
                     NLYLEYSQVAAGKQLIPVSPFDFKQSIVIVLPTYNERANLEALVPAISQYLVADILIV
                     DDNSPDGTGELADRLSCKHGHVHALHRLKKEGLGPAYLAGFKWALQRNYSLIIEMDCD
                     FSHAPWDLPRLVYRSRTADLVIGSRYVPGGGTENWNASRRFVSRCGNTYVRLFLGSNI
                     HDWTGGFRCYRRELLIRMNLATVRTKGYIFQVELAWRAVQLGADVDELPIRFCDRIEG
                     QSKLGWQSIAEALIEVPKMYLLRLDD"
BASE COUNT          453 a          496 c          602 g          451 t
ORIGIN      
        1 cacggaatgg gtggaccggg ctataaggtg aaggcggagt tcaacagtac gccgcacaag
       61 cgaggcgttg tttcgatggc ccgcgcgaat gatccggata gcgctgggtc acagttcttt
      121 atttgcgtcg cagacgccaa tttcctcgac tggcaataca cggtatttgg ggaagtggtc
      181 agcggaatgg aggttgcgga caagatcgtc catatgaagc gtgatggccg agacaatccg
      241 ctggagcgag ccgagatgac cgtcgcgata agtgaaggtt gagctaaggc agagattaat
      301 gtcgaacatg ccgttgaacc tccactgtct gatcttaacc ctgacctcag ccttcctgct
      361 gttcggttgt gcggctcctc atataccatc gcgaattatt tatgaagatc cagtcaattt
      421 cgtgcggttg gaggaagacc ctgaagtgct cgcggaatgg ccgccaagcc accatagcca
      481 tccattcacg atcgaaccgg aaaagttgcg gacgattttg tcgggattga aggtgcagga
      541 acattggatt gcgctgcagc ggtggatgag gggggagtct cctctggttc cggccttcac
      601 ggatgaggag ttggctctct tgtctgtacg catagcggag gcgctggcgg aagcgaaaga
      661 taacgagcgg gttaccttct atttgagcga accgcagaca ttcgcgaggc gaattattac
      721 gacgggcgga ctgtacgttc atgggacaca actgcatatc ctgttgggaa attggagaat
      781 catttacggc atcccggcct atggaatgat ctatgatcgg cgttacccga tgcgaccgac
      841 tgcggcgaag ggattcgatt tgacttttca gccgtccgaa gccgtgattc cgatgaagag
      901 tagcttgctg gacggagttt tggccaatgc gaaggacgaa cttatcatcg acttgaccaa
      961 gctcgattcg cccgatccgg agattactcg tagggtctta tgtgactgat gagtcactat
     1021 gtgagcgacc gctggcgaca cggcaaggag gggtcgccgg ggggaatgga aagaggaaga
     1081 tgaacggctt atcaatcgtc caggcgcagg aggtacattt tgggtacttc tatcagcgct
     1141 tcggcgatgg actgccagcc gagcttgctt tggccttcaa tgcggtcgca aaagcggatc
     1201 ggcaattcgt ccacatccgc acctagctgt acggctcgcc aggccagctc tacttgaaag
     1261 atgtatcctt tcgtccggac agttgcgagg ttcatccgta tcaagagctc acgacgatag
     1321 cagcggaatc ctcctgtcca gtcgtggatg ttcgaaccga ggaagaggcg gacgtaggta
     1381 ttcccgcagc gagagacaaa acggcggctt gcgttccagt tttcggtccc cccgccgggc
     1441 acataacgac tcccgatcac caggtcggca gttcggctgc ggtataccag gcggggcaga
     1501 tcccaggggg catggctgaa gtcgcaatcc atttcgatga tgaggctgta attgcgttgc
     1561 agcgcccact tgaagcccgc cagatacgcc ggtccaaggc cttctttttt caatcggtgc
     1621 agagcgtgta catgtccatg tttacaacta agccgatcgg ctaactcccc ggttccatcc
     1681 ggtgagttat catcaacgat caagatatcg gccacaagat attggctgat cgccgggacg
     1741 agcgcctcaa gattggcgcg ttcattgtaa gttgggagga caatgacaat cgattgtttg
     1801 aaatcgaacg gtgagacggg aatgagctgc ttacccgctg ccacttgaga atactcaagg
     1861 tagaggtttt cggcatcgtg tcggctgatg gcaatgcgtg cgctgtcggc acctcgggcg
     1921 atcagttcct cggcaagctc gaagtccgga acggcataag cttcatctgg gcccagctct
     1981 ttaagaggaa ggatgccgtt gc
//
LOCUS       DATMTI010000709         9085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_663260, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000709 DATMTI010000000
VERSION     DATMTI010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663260"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /gene="nuoH"
                     /locus_tag="VN039_11380"
     CDS             complement(<1..768)
                     /gene="nuoH"
                     /locus_tag="VN039_11380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWV46601.1"
                     /translation="MIDWGRTGLIIATMLGGLLTLAALLIWVERRLLGIWQERYGPNR
                     TGPGGILQSLADMIKIFTKEDWIPPFADKVVFVLTPAIVIVTMLMAFAVVPIAPGFVV
                     ADINIGVLFFLAMSSLAAYSVIIGGWASNNKFAFLGSLRATAQLLSYEVFMGLSLMGS
                     VLLAGSFNLSDIVEAQRHLWFVVPQFLGFVGFFIAGLAEAHRAPFDLPEAETELVAGY
                     HAEYSGMKFGMFFVGEYLSITLLSAMIVILFFGGWHGP"
     gene            complement(881..3628)
                     /gene="nuoG"
                     /locus_tag="VN039_11385"
     CDS             complement(881..3628)
                     /gene="nuoG"
                     /locus_tag="VN039_11385"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWV46602.1"
                     /translation="MTSIIVDNRPYTVHEGQNLLAACLGAGLNLPYFCWHPAMGSVGA
                     CRQCAIKQFKDDRDQRGRLVMACMTPASDGARISIEDSEAKAFRASVVEWLMVNHPHD
                     CPVCDEGGECHLQDMTVMTGHAYRRYRFPKRTYRNQYLGPFIKHEMNRCIQCYRCVRF
                     YRDYAGGRDFNVFGSHDAIYFGRERDGVLENEFSGNLIEVCPTGVFTDNTLFHHYTRK
                     WDLQSAQSICPHCSLGCNTTPGERYGILRRVVNRYNSQVNGYFICDRGRFGYEYVNSD
                     GRIRRALLRPKRDRPARPEGVPDVLAAAAHRLRRARGIVGIGSPRASLEANAALRSLV
                     GADRFFQGIGDREHRLLSTILTILRTGPVPSASMREVEESDAVLVLGEDLLNSAPRLA
                     LALLQSVRQQPMKRVDELKIFRWDDAAARNIVQDRRGPLFLAGYRRTWFHDYATECYF
                     AAPDDIARLGFSVAQSIGTDNSAVPNLSKEVRDRAHRIAETLTRAERPLVIAGTESGS
                     QATIEAAANVAWALQSIGKHPRLSFVLPDCNSLGLALLGGGTLKEAFESVRQKQADGV
                     IVLENDLYRHTKPAEADAFFGTAKTVVAIDSFDHRTAARADILLPAATFPESDGTLVN
                     QEGRAQRFYQVFVPEGDITESWRWLRDLARATSNQAEDLSGVATWQTFDDAVSGTAEA
                     VSEFAKIREVAPSAGLRLVGQKIPRQPERYSGRTAMISHLTVHEPPPPPDPDTPLAFS
                     MEGYRGPVVSPLIPQVWAPGWNSVQALNKYQDEVGGPLREEMPGVRLIEPMATKAPFW
                     FTNIPPPFRPAYHRWLLLPMPALFGSEELSNQSPAIVERTSEPFIALHPSDGARLRLE
                     EGMAIRLTLREATYRLAVRFDSSTPPGTAGLSLAHEATWMRLPAWVDLTESIKADRRV
                     A"
     gene            complement(3633..4937)
                     /gene="nuoF"
                     /locus_tag="VN039_11390"
     CDS             complement(3633..4937)
                     /gene="nuoF"
                     /locus_tag="VN039_11390"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWV46603.1"
                     /translation="MEQPLTQHIARDDRYRSLSEYNKTGGYRSLKRLADGLSPQDIQR
                     LVTESSLRGRGGAGFPTGSKWSFVPMGSDAPRPKYIVANADEMEPGTFKDRVLMEGDP
                     HGLVEGMILAGYAVQAEVGFIFLRGEYHLSAQYLTNAVSEAYKEGHLGRTLSGTDFRF
                     DLHLHTSAGRYICGEETALLNALEGKRAIPRAKPPYPQAAGLWGRPTVVQNVETLYNL
                     PHIVNHGSTWYHGLSRSKDGGTKLYGMSGRVAHPGCWELPLGTTARELLEQYADGMFD
                     GFGFRGLLPGGASTAFLTEEHLDLPMDFSSIPPQVGRLGTGTMIVLDDRTCPVGFVLN
                     LEKFFARESCGWCTPCREGLPWVARILQLFEEGRATHDDLTLLEMHSTLLGPGHTFCA
                     LAPGAVEPLKSALKYFRDDFVRHIRLGQCPWNVTLKRQKVLV"
     gene            complement(5033..5497)
                     /gene="nuoE"
                     /locus_tag="VN039_11395"
     CDS             complement(5033..5497)
                     /gene="nuoE"
                     /locus_tag="VN039_11395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HWV46604.1"
                     /translation="MLSDIERGEIEEALKHYPDKRAGTIDALLLLQRHRGWVSDQSLL
                     DIAQFLDMTREDVDGVATFYNLIFRQPVGRHILFMCDSISCWIMGYERVRQQLTSRYG
                     VDMGQTTNDGRLTILPIACLGHCERAPAIMMDGDVYGNVTPDKIECIVEKYK"
     gene            complement(5560..7350)
                     /gene="nuoC"
                     /locus_tag="VN039_11400"
     CDS             complement(5560..7350)
                     /gene="nuoC"
                     /locus_tag="VN039_11400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF008728.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HWV46605.1"
                     /translation="MNHRPSTIEDTPVSGSDVVEELKGRLGSEGILVFQPTRDEIPTI
                     WVDTNRLRETLQYLRSEISRPFPMLFDLSAIDERLRRNHDGLPIGLFTVFYHLYSFDR
                     KQSIRLKVSLRDEPLSLPSVVALWPNADWYEREVYDMFGIQFEGHPFLRRLLMPPWWN
                     GHPLRKDHPARGTEMGRFQLPPEKLAMAQEALQFNPDDWGLARRHHDPDFDYMFINLG
                     PAHTGTHGVLRLVTQLAGEEIIDIVPDIGFHHRAKEKIAERQTWHTFIPYTDRVDYLQ
                     GVLNEMPYCLSVERLAGITVPDRAQVIRVMMCEFYRLASHLVWYGTFAQDVGALSPVF
                     YMFNDRERIHAVTEAICGGRMHPNWFRIGGVSQDLPVGWDKLVRQFVDYLPARLDEYE
                     RLVMDNPIFKQRTVGIGAFTLEDAMAWGATGPMLRACGLPWDLRKVRPYSGYDQFDFD
                     VPVGSHGDCYDRAVVHVLEMRQSLRIIRQCLEHMPTGPYKSLDPLATPPLKERTMHDI
                     ETLIHHFLGVSWGPVLPPGEAEVPTESSKGQTSYYLTSDGAQVSYRTRIRTPSFAHIQ
                     MVPFMARGELLSDLLAVLGSVDFVLSDVDR"
     gene            complement(7347..8054)
                     /locus_tag="VN039_11405"
     CDS             complement(7347..8054)
                     /locus_tag="VN039_11405"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWV46606.1"
                     /translation="MHGWRNGRLEGVSIRDGGDGSLTTVIGQSVLLAKLQDLLAWGRK
                     NSLWPLNFGLSCCYVEMATSLTSVYDVARFGAEVVRGSPRQADVLVVSGTVFIKVAPI
                     IKQLYEQMMEPRWVISMGSCSNSGGMFDAYSVVQGVDKFLPVDVYMPGCPPPPHAFME
                     CLLLLQDKVGKERRPLSWWFGPQEVQKPIMPSMRDAKREERVRRREYPTTDILPRTDL
                     LSPHFAGELLDRPASQP"
     gene            complement(8038..8493)
                     /gene="ndhC"
                     /locus_tag="VN039_11410"
     CDS             complement(8038..8493)
                     /gene="ndhC"
                     /locus_tag="VN039_11410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWV46607.1"
                     /translation="MPLQWMLQLGLYAVGVLIVIAVMLGLPALLGERYWKKPERRRDI
                     ATGVPYESGIQPTGTAQIRLPIQYYLVAMFFVIFDLEAVFLYAWAVAVKETGWLGFIE
                     VVIFVAILLAALAYLWLIGALDWSARYQRPSLHRVHRLQAKERGNARVA"
     gene            complement(8772..8951)
                     /locus_tag="VN039_11415"
     CDS             complement(8772..8951)
                     /locus_tag="VN039_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HWV46608.1"
                     /translation="MLLKWAFMFFVIALIAAAFGFTGIAAGAASIAKILFYLFLGIFF
                     IFLIAGIILGHKVFS"
BASE COUNT         2001 a         2600 c         2656 g         1828 t
ORIGIN      
        1 cggcccgtgc catccgccga aaaataggat cacgatcatc gccgagagca acgtgatgct
       61 caggtattcg ccgacgaaaa acatgccgaa cttcatgccg gaatattcgg catggtaacc
      121 cgccaccagt tccgtttccg cttccggaag atcgaacggc gcgcggtgcg cttcagccag
      181 tccggcgatg aaaaaaccga caaaccccag aaactgaggg acgacaaacc aaaggtgacg
      241 ttgggcttca acgatgtcgc tcaagttgaa ggagccggcc agcaggaccg atcccatcag
      301 cgacagaccc atgaagactt cgtagctcaa gagttgagcg gtggcgcgaa ggcttccgag
      361 aaatgcgaac ttgttgttcg acgcccagcc cccgatgatg acactgtaag cggcaaggct
      421 ggacatggcc aaaaagaaca ggaccccgat gttgatgtcg gctacgacaa agcctggtgc
      481 gatcgggacg acggcgaacg ccatcaacat tgtgacgatg acgatggccg gcgtcagcac
      541 gaagacgacc ttatcggcga acggcggaat ccaatcttct ttggtgaaaa ttttgatcat
      601 gtcggcgagg gactgcagaa tcccgcccgg tcccgtacgg ttcggtccat agcgttcctg
      661 ccagattcca agcagccggc gttcgaccca gatcaaaagg gcggcgaggg tgagcaggcc
      721 accgagcatc gttgcgatga tgaggccggt tcgcccccag tcgatcacgc catcctccgg
      781 tcggttttga tcgattcgtt aagattcacc cgagtcggca attgttccca ggccatttca
      841 tgcaccagag cgatgatacg tgcagtgtat tcccagtcga tcatgccacc ctccggtcgg
      901 ctttgatcga ttcggtgagg tcgacccacg caggcaatct catccaggtt gcttcatgcg
      961 cgagggacag gcccgccgtg ccgggcggcg tggacgagtc gaagcgtacg gccagacggt
     1021 atgtggcttc ccgcagcgtc agtctaatcg ccattccttc ttcaagacgg agtcgagcgc
     1081 cgtccgatgg atgcaatgca atgaaaggtt ccgacgtccg ttcgacaatg gccggcgatt
     1141 gattgctcaa ttcctcgctt ccaaaaagag cgggcatcgg caacagcagc catcggtgat
     1201 atgcgggccg aaagggaggc gggatgttcg tgaaccaaaa tggagccttc gtggccatcg
     1261 gctcaatcag ccgcacaccc ggcatttctt cacgtagcgg gcctccgact tcgtcctgat
     1321 atttgttcaa ggcttggacg gagttccatc cgggagccca gacctgcgga atgagcggag
     1381 agacaaccgg gcctctgtac ccttccatgg aaaaggccag cggagtatcg gggtcgggcg
     1441 gcggtggcgg ttcatggacg gtgagatggg aaatcatggc ggtgcgcccg ctataccgct
     1501 cgggctgtcg ggggatcttt tgtccgacca agcggagacc ggcagaggga gcaacctctc
     1561 tgatcttcgc gaactctgaa acagcctccg ccgtcccgga gacggcatca tcgaaggttt
     1621 gccaggttgc cacaccggat agatcttctg cctggttcga tgtcgctcga gcaagatccc
     1681 tcagccagcg ccagctttcc gtgatatcgc cttccggcac gaagacttgg taaaaccgct
     1741 gggctcggcc ttcctgattg acgagggttc catcagattc cgggaatgtt gctgccggca
     1801 gaaggatgtc ggcgcgagcc gccgtgcgat gatcaaagga gtcgatggcg actacggttt
     1861 ttgccgtgcc gaaaaaggca tcggcttcgg cgggtttggt gtgacgatac aggtcgtttt
     1921 ccaagacgat cactccgtcc gcctgtttct gccggacgga ctcaaacgcc tccttgagcg
     1981 ttccaccgcc caggagagcg agtcccaggc tattgcaatc gggcaggacg aagctgagac
     2041 ggggatgctt ccctatagat tggagagccc aggcgacatt ggcggcggct tcgattgtcg
     2101 cttgagaacc gctttcggtg ccggcgatca ccaacggccg ctcggctcgc gtcagggttt
     2161 cagcgatgcg atgggccctg tcgcgtactt cttttgagag attcggaacg gcggagttgt
     2221 ccgtgccgat ggactgggcg acggagaacc ccagtctggc aatatcgtcc ggcgcggcga
     2281 agtaacattc cgtcgcataa tcgtggaacc aggtacgtcg atagcctgcc aaaaagagcg
     2341 gtcctcgtct gtcctgcacg atgttgcgcg ctgcggcatc gtcccagcgg aatattttca
     2401 gttcatccac ccgcttcatc ggctgttgtc gaaccgattg caggagggcg agcgccaaac
     2461 ggggagcgct gttcagcaga tcttcaccga gaacgaggac ggcgtccgac tcttctactt
     2521 cacgcatgga agcagacggc accggaccgg tccgcagaat ggtcaggatc gtcgagagaa
     2581 gtcggtgctc acggtctccg atgccttgaa aaaacctgtc ggcaccgacg agcgagcgga
     2641 gagcggcatt cgcctccaat gaagcgcggg gtgagccgat tcccacgatc ccgcgcgccc
     2701 gccgtaaacg atgggccgcg gcggcgagca cgtcggggac tccctcaggt cttgccgggc
     2761 ggtcacgctt cggtctcaag agggcgcgtc taatccggcc gtcgctgttg acatactcgt
     2821 atccgaatcg accacggtca cagatgaagt acccattgac ctgagagttg tagcggttga
     2881 ccactcggcg caagataccg tagcgctctc ccggtgtagt attgcaccct aagctgcaat
     2941 gggggcagat cgactgggcc gattgcagat cccatttcct cgtgtagtgg tggaacagcg
     3001 tattatcagt gaacacaccg gtcgggcaga cctcgatcaa attgccgctg aattcatttt
     3061 ccagaacgcc gtcccgttcc ctgccgaaat agatggcatc gtgggaaccg aatacgttga
     3121 agtcacgtcc acccgcatag tctcggtaga accgcacgca gcggtaacat tgaatgcagc
     3181 ggttcatctc atgcttgatg aaggggccca gatactgatt gcgatacgtc cgtttgggaa
     3241 accggtagcg acggtaggca tggcctgtca tgacggtcat gtcctgcaga tggcattcac
     3301 cgccttcatc gcagaccgga caatcgtgcg gatggttgac catcagccat tcgacgaccg
     3361 aggctcgaaa ggccttggct tcagaatcct cgatcgaaat gcgggcgccg tcggaggctg
     3421 gtgtcataca agccatgacc aggcgaccgc gttgatcacg gtcgtctttg aactgtttga
     3481 tcgcgcactg acggcaggcc ccgaccgatc ccatggcggg gtgccagcag aaatacggca
     3541 ggttcagccc tgcccctaag caagccgcga gcaggttctg tccctcatgg acggtgtaag
     3601 gccggttatc aacaatgatg gaagtcatag ggctacacca acaccttttg ccgtttcagc
     3661 gttacattcc atggacattg tcccaagcgg atatgccgga caaagtcgtc gcggaaatat
     3721 ttcaacgcgg atttcaaagg ttccacagcg cccggtgcca acgcacagaa ggtatgtccg
     3781 ggaccgagga gtgtcgaatg catctccagc aacgtcagat catcgtgggt ggctcgtcct
     3841 tcttcgaaga gctgtaatat acgtgcgacc cacggcagcc cttcccggca aggcgtgcac
     3901 cagccgcagg actcgcgggc aaagaacttt tccagattga ggacgaaccc gaccggacag
     3961 gtacggtcgt cgagcacgat catggtgccg gttcccagcc ggcccacttg tggtgggatc
     4021 gaggaaaaat ccatggggag atcgaggtgc tcttcggtga ggaatgccgt ggaggcgccg
     4081 ccggggagta gtccacgaaa gccgaagccg tcgaacatgc cgtcggcata ctgttccagc
     4141 agttcgcgtg ccgtcgtccc gagagggagt tcccagcatc ctggatgcgc aacgcggccg
     4201 ctcatgccgt agagtttcgt gccgccatcc ttactgcggc tgagaccgtg ataccacgtc
     4261 gagccatggt tgacgatgtg aggaaggttg tacaacgtct cgacattctg aacgacggta
     4321 ggtcggcccc acagaccggc cgcctgaggg taaggaggct ttgcacgggg aatcgcccgt
     4381 ttgccttcca aggcattgag cagcgctgtt tcctctccac agatgtaacg gcccgcgctg
     4441 gtatggaggt gaagatcgaa gcgaaaatcc gttccagata gtgtccttcc gagatgccct
     4501 tccttgtagg cttcggagac ggcgttcgtt aagtattgag cagacagatg atactcgccg
     4561 cgtaggaaga tgaagccgac ttccgcctgt acggcataac cggcgagaat catcccttcc
     4621 accaggccgt gaggatctcc ctccataagc acgcgatctt taaacgtgcc cggttccatc
     4681 tcgtcggcat tggccacaat atatttcggc cttggcgcat cggatcccat cgggacgaag
     4741 ctccacttgc tgcccgtcgg gaatccggcg ccacctctcc cacgcagact cgattccgtc
     4801 accagccgtt ggatgtcttg aggggacagc ccatcggcaa gccgcttgag gctgcgatag
     4861 ccgccggtct tgttatattc ggataaggac ctataccggt cgtcccgggc gatgtgttgg
     4921 gtgaggggct gttccatgtg aaacctgttt gaggttcgag gcagaaggct aagtgaacag
     4981 ccgttgtctc aatctcaact tctaacctcc agcctccaac ctcaggcctt ctctatttgt
     5041 atttctccac gatgcattca atcttgtcgg gcgtcacgtt gccgtagaca tcgccgtcca
     5101 tcatgattgc cggcgcccgt tcgcaatgcc cgaggcaggc aatcggcaga atcgtcagcc
     5161 gaccatcgtt cgtcgtctgt cccatatcca cgccatagcg actggtcaac tgctgtcgta
     5221 cccgctcgta tcccatgatc caacaactga tgctgtcgca catgaagagg atatggcggc
     5281 cgacaggctg gcgaaagatc aggttgtaaa aggtcgccac cccatccaca tcttcacgtg
     5341 tcatgtcgag aaattgtgcg atatcgagga gggattgatc cgagacccat cctctatgcc
     5401 gctggaggag caacaaagca tcgatggtac cggctcgttt gtcggggtag tgtttcaagg
     5461 cctcttcgat ttcgccgcgt tcaatgtcgc tcagcatatg tgctccgcac tcgtgaggcg
     5521 tgaaacgtca ggcgttaggc gtgttggaaa ttcacgctct cacctgtcca catccgacag
     5581 gacaaagtcg acgctgccca agacggccag cagatcggac agcaactcgc cgcgcgccat
     5641 gaacggcacc atctgaatgt gcgcgaacga cggagtgcgg attctggttc ggtacgaaac
     5701 ctgtgccccg tcgctggtca ggtaataact ggtctggccc ttcgacgatt ccgtcgggac
     5761 ttccgcttcg cccggcggaa gcaccggccc ccaactcacc cctaggaagt gatggatgag
     5821 cgtttctata tcgtgcatgg tgcgttcctt cagaggaggc gtggcaagag ggtcgagcga
     5881 tttgtaggga ccggtcggca tgtgttcgag gcattgccga atgatgcgca acgactgtcg
     5941 catctccagc acgtgcacca ccgcccgatc gtaacagtcc ccgtgagaac cgaccggaac
     6001 gtcaaagtcg aactgatcgt agccggaata agggcgaact tttcgcagat cccaagggag
     6061 tccacaggcg cgcaacatcg gtccggtggc gccccaggcc atggcgtctt cgagcgtgaa
     6121 ggcgccgatg ccgacagtcc gttgcttgaa aatcggattg tccatcacca gacgctcata
     6181 ttcatccagg cgtgcaggca agtagtccac gaactgtctg accaatttgt cccatccgac
     6241 aggaaggtct tgggacacgc cgccgatacg gaaccaattc gggtgcatgc ggccgccgca
     6301 gatggcttcc gtgacggcat ggatccgctc gcgatcgttg aacatgtaga agaccggcga
     6361 gagggcgccg acgtcctgcg caaaggtgcc gtaccacacg aggtggctgg cgaggcggta
     6421 gaactcgcac atcatgacgc ggatgacctg cgcccggtcg gggaccgtga tgccggccag
     6481 tcgttcgacc gagagacagt agggcatttc gttgaggacg ccttggaggt agtcaacccg
     6541 atcggtatag ggaatgaacg tgtgccaggt ctgccgctcg gcgatctttt ctttggcacg
     6601 atggtggaac ccgatatcgg gcacaatgtc gatgatctct tcgcctgcga gttgcgtgac
     6661 gagccgcaag acaccatgtg tgccggtatg ggcgggaccg aggttgatga acatataatc
     6721 gaagtccgga tcgtgatgcc tgcgggccaa cccccagtcg tcgggattga actgcaacgc
     6781 ctcctgtgcc atggcgagtt tttctggcgg cagttggaac cggcccattt ctgttccgcg
     6841 tgcgggatgg tctttccgga gcggatgacc gttccaccac ggaggcatga ggaggcggcg
     6901 gagaaacggg tgcccttcga actgaatgcc gaacatgtca tagacttcgc gctcgtacca
     6961 atcggcattg ggccaaagag ccacgaccga gggcaacgac agcggttcat cgcgaagcga
     7021 cactttcagg cgtattgatt gttttcgatc gaatgaatag agatgataaa aaacagtgaa
     7081 gagaccgatc ggcagtccgt catggtttcg gcgtaaccgt tcgtcgatcg cgctcaaatc
     7141 gaagagcatc ggaaacggac gagaaatctc cgatctcaaa tactgaagtg tttcacgcag
     7201 ccgattcgta tcgacccata tagtcgggat ctcatctctt gtgggttgga agaccaagat
     7261 tccttcggaa ccgaggcgac ccttgagttc ctctaccacg tcagaaccac tgaccggcgt
     7321 gtcttcaatg gttgatggtc gatggttcat ggctgcgagg ccggtcgatc gagaagttct
     7381 cccgcaaagt gcggcgaaag tagatcggtt ctgggaagaa tgtccgtggt cggatattcc
     7441 cgccgacgga cccgttcttc ccgttttgca tcccgcatcg acggcataat gggtttctgg
     7501 acttcctgtg ggccgaacca ccagcttaaa ggccgtcgct ccttgccgac tttgtcttgc
     7561 aacaacagca ggcactccat gaatgcatgg ggtggcggag ggcaacccgg catgtagaca
     7621 tccaccggca ggaacttgtc cacgccctgc acgaccgagt atgcatcgaa catcccgccg
     7681 gagtttgagc aggaacccat cgagatcacc caccgcggtt ccatcatctg ttcgtacagc
     7741 tgtttgatga tcggagcgac cttgataaac acggttccgg acacaacgag cacgtcggct
     7801 tggcgcggag agccgcggac aacttcggcg ccgaacctgg ccacgtcata cacgctggtg
     7861 agactcgtcg ccatttcgac atagcaacag gacagcccga agttcagcgg ccataacgaa
     7921 ttctttcgcc cccaagccag cagatcttgc aattttgcga gtaacaccga ctggccgatc
     7981 acggtggtca gggatccgtc ccctccgtct ctgatggaaa cgccctcaag tcgtccgtta
     8041 cgccacccgt gcattgcccc gctccttggc ttgcagccga tgaacgcgat ggagagacgg
     8101 tcgttggtat cgcgcgctcc aatccagcgc gccgataagc cagagataag ccagggctgc
     8161 caagagaatc gcgacaaaga tcaccacctc gatgaagccg agccaacctg tctccttgac
     8221 ggccaccgcc caggcataga gaaagacggc ttccaagtcg aaaatcacaa aaaacatcgc
     8281 gacaaggtag tactggatgg gaaggcggat ttgcgcagtc cccgtgggct ggatacccga
     8341 ctcatacggg acgccggtgg cgatatcccg tcggcgctca ggctttttcc aatatcgctc
     8401 ccccaggagt gccggtagtc ccagcatcac cgcgatgacg atgagaacac ccaccgcata
     8461 gagtccgagt tgcaacatcc actgcaatgg catccaagcc tccatttgat cgcgagttca
     8521 tgtcgtgacg gtcttcaagc caaaagtatc gctaagcaag gggcaagcca gtatccggat
     8581 cgacaaaggc tagcggggga caatcgtcag cctaccgatt gctcgggatg agcatttatt
     8641 gcagacagtt tgtccgaggc gtggacagtt tgtcttgccc gaataacccg agcagccttc
     8701 tatagaccgc ttctccagtg atgttgagcg gtattgtaga aggccggaaa gcacccgaga
     8761 cagcggcttc gctaagaaaa cactttgtgc cccaggataa tgccggcgat cagaaagatg
     8821 aaaaaaatcc ccaaaaacaa atagaaaagg atcttggcga tactcgcggc tcctgcggcg
     8881 atgccggtaa agccgaacgc ggccgcaatc aacgcgatca caaaaaacat gaaagcccat
     8941 ttcaaaagca tagccccctc ttccagtgtc actgtacatg gcgcaatatc gatgcccggt
     9001 ccggtctcgc aggcgaggtc tcaaaacgga ggatgttcga tcgtgccgaa tcggcaggag
     9061 cgaggtcttg ctgtgcgaac aatgg
//
LOCUS       DATMTI010000710         3578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_834188, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000710 DATMTI010000000
VERSION     DATMTI010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834188"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..1323)
                     /gene="nadA"
                     /locus_tag="VN039_11420"
     CDS             complement(220..1323)
                     /gene="nadA"
                     /locus_tag="VN039_11420"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWV46609.1"
                     /translation="MTVTAAVSRPITEYQTQATEELFRRTAAAKSTLGERVMILGHNY
                     QRDEVIQHADFRGDSLLLAKLAAKRSERPYIVFCGVHFMAETADILSRSQQTVILPDM
                     AAGCSMADMAAIEQVDQCWEALGRVVPVEDTVMPAVYVNSAAVLKAFCGEHGGITCTS
                     SNAKAVIEWCWTRREKILFFPDEHLGRNTANKMGIPREQMIVWDPYQPNGGNTKEAIK
                     RAKLILWKGHCSVHQMFQPVHVDHFRKQYPDGNVIVHPECHEDVVNKADLIGSTEFII
                     RTVTAAPAGTTWAIGTELNLVNRLKHELTDKKVFFLSSTVCQCATMFRIDAAHLCWAM
                     ENLAEGHVVNHIVVPEDDKQWAKVALDRMMTVS"
     regulatory      1492..1594
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1707..>3578
                     /gene="thiC"
                     /locus_tag="VN039_11425"
     CDS             1707..>3578
                     /gene="thiC"
                     /locus_tag="VN039_11425"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HWV46610.1"
                     /translation="MSIQTNTHGSVNGNGIPTSPLTTTPFPASGKVYVNGTQSGVRVP
                     MREISLTQTMTQTKGTNGTRSNNAPVIVYDTSGPYTDPAVTIDVRKGLIPLRRPWVLS
                     RQDVEELPQVSSTYGRIRATDPKLAELRFQHIRKPLRAKPGTNVTQLHYARKGIVTPE
                     MEFIAIRENQSCRTEAEAASQNGHGGGVKQHPGFAWGAAIPRVITPEFVRDEVARGRA
                     IIPSNINHPESEPMIIGRNFLVKINSNIGNSAVASSIEEEVEKMIWSTRWGADTVMDL
                     STGKNIHETREWIIRNSPVPIGTVPIYQALEKVTGKAEDLTWEIFRDTLIEQAEQGVD
                     YFTIHAGVRLAYVPMTAKRMTGIVSRGGSIHAKWCLAHHQENFAYTHFEEICEIMKAY
                     DVAFSLGDGLRPGSIADANDEAQFAELETLGELTKIAWRHDVQVMIEGPGHVPMHMIQ
                     ANMEKQLEACHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHL
                     GLPTREDVKTGVITYKIAAHAADLAKGHPGAQARDNALSKARFEFRWEDQFNLSLDPE
                     TAQQFHDETLPDNAAKVSHFCSMCGPHFCSMKITQDVRDYAAQLQVDEQRAIQIGMNE
                     "
BASE COUNT          831 a         1022 c          963 g          762 t
ORIGIN      
        1 tcccttcccc acgacttgaa tatggtcata gagtttctgc tgctcccacc aggccaacat
       61 ttcaggctcg cgctgcggca gattggcctt catcgggaag tcggttttcg gaagattgag
      121 cgttgatttg taatccatag acaatgggac ccgggcattc accggatgat catacgggat
      181 cgatggacta taccggaagg aaggggggag gcaccagccc taactgacgg tcatcatgcg
      241 gtcgagcgca accttcgccc actgcttgtc gtcttcgggc accacgatgt ggttcacgac
      301 atggccttcg gccagattct ccatggccca gcagagatgc gccgcatcga ttcggaacat
      361 ggtggcgcac tgacagaccg tggatgacag gaagaacact tttttgtcgg taagttcgtg
      421 cttcagtcga ttgacgagat tcaattccgt cccaatcgcc cacgtcgtgc cggccggcgc
      481 ggcggtcacc gtacgaatga tgaactcggt cgatccgatg agatccgcct tattgaccac
      541 gtcttcgtgg cattctggat gcacaatgac gttgccgtcc ggatactgct tgcggaagtg
      601 atccacatgg accggttgaa acatttgatg gacgctgcaa tgacctttcc ataagatcaa
      661 tttggctcgt ttgatggctt ccttggtatt cccgccgttg ggttggtagg ggtcccatac
      721 gatcatctgt tcgcggggaa tgcccatttt gttcgccgta ttacggccca agtgttcatc
      781 aggaaagaag agaatttttt ctcgcctggt ccagcaccat tcaatcacgg ctttggcatt
      841 ggaagacgtg caggtgattc ctccatgctc accacaaaac gccttgagca ccgcagcgga
      901 gtttacgtag accgccggca tcaccgtgtc ttccacggga accacgcgtc ccaatgcttc
      961 ccagcactgg tcaacctgtt cgatcgccgc catatcggcc atcgaacaac ccgcagccat
     1021 gtccggcaga atcactgttt gttgagaacg gctgaggatg tccgccgtct cggccataaa
     1081 gtgcacgcca caaaaaacga tgtaagggcg ctcggagcgt ttggcggcca attttgccaa
     1141 caaaagcgag tcaccgcgaa aatcggcgtg ctggatgact tcgtcccgct ggtaattgtg
     1201 gcccagaatc atcactcgct caccaagcgt gctcttcgcc gctgccgtcc gacggaataa
     1261 ttcctccgtc gcctgcgtct ggtactcggt gattggcctc gatactgccg cagtaaccgt
     1321 cacaatatgc ctccaatcat aaagaaattt attctacgat agccttccct cacaatcaac
     1381 gaatagccct atgaggaaaa gtcctagtcg gtcgcccaaa ggatggtagg cggaatgggt
     1441 ctaataaccg attgacttga ctcatctccg aggaatacga tcagcgtcaa tagctagagc
     1501 taggggggcc ttgaggctga gagtccgaat gatcggacga ccctcacaac ctgacctggg
     1561 taataccagc gtagggaagc cggccgaaag cggatctcgt gaggttcagc cgcgttccct
     1621 accctgggaa agcggctttt tttattgcag catgccacgt cagcttttgg ctccgcatat
     1681 cgaccaacca tgaaaggatc tcttatatga gcatccagac aaatacacat ggatcggtta
     1741 acgggaatgg gatcccgact tctccattga ccacgacgcc ctttccggca tcgggcaagg
     1801 tctatgtgaa tggtacgcag tcaggggtgc gcgtcccgat gcgggaaatt agtttgaccc
     1861 agactatgac ccagacgaag gggacgaatg ggacgagatc aaacaatgct cccgttatcg
     1921 tctatgatac ctctggtcct tatactgatc ccgccgtcac gatcgacgtc cgtaagggac
     1981 ttatcccatt gcgacgcccc tgggtactca gccgacagga tgttgaagag cttccacagg
     2041 taagctcgac ctacggccgc atccgtgcga ccgatccgaa gttggccgag cttcgctttc
     2101 agcacattcg taaacccttg cgggcgaagc ccggtacaaa cgtgacccaa ctgcactatg
     2161 cgcgaaaagg cattgtcaca ccggaaatgg aatttatcgc catccgggag aaccaatcat
     2221 gccgaaccga agccgaggct gcatcccaga acggccatgg cggtggtgtg aagcagcatc
     2281 ctggatttgc ctggggtgcc gctattcctc gggtcatcac gccggagttc gtgcgcgacg
     2341 aagttgcccg tggccgcgcg atcatcccgt cgaacatcaa ccatccggaa agcgagccga
     2401 tgatcatcgg ccggaacttt ctcgtgaaga tcaattccaa catcggcaat tccgccgtcg
     2461 cctcatccat cgaagaggaa gtcgagaaaa tgatctggtc gactcgctgg ggcgccgaca
     2521 ccgtgatgga cttgtcgacc ggcaaaaaca ttcacgaaac acgcgagtgg atcatccgca
     2581 attcaccggt gccgatcgga acggtgccga tctatcaagc gcttgaaaaa gtgaccggca
     2641 aggcggaaga cctgacgtgg gagatttttc gcgataccct gatcgaacag gccgaacagg
     2701 gcgtcgacta cttcacgatt cacgccggtg tgcgccttgc ctatgtgccg atgaccgcca
     2761 agcgaatgac cggtattgtt tcgcgcggcg gttcgatcca tgcgaaatgg tgcttggctc
     2821 atcatcaaga gaacttcgct tacactcact tcgaagagat ctgcgagatt atgaaggcct
     2881 acgacgtggc tttcagtctc ggcgacggac tccgaccggg atcgattgcc gatgccaacg
     2941 acgaagcaca atttgccgag ctcgagacat taggcgaact gaccaagatc gcatggcggc
     3001 atgatgtgca ggtcatgatc gaaggaccgg gccatgtgcc catgcacatg attcaagcca
     3061 atatggaaaa gcagcttgag gcctgccatg aggcgccgtt ctatacactg ggaccgctga
     3121 cgaccgatat tgcacccggc tatgaccaca ttacatcagg catcggcgcc gccatgatcg
     3181 gctggtacgg ctgcgccatg ttgtgctatg tgactcccaa ggaacatttg ggcttgccga
     3241 cccgcgagga cgtcaagacg ggcgtcatca cctataaaat tgccgcgcat gcggccgatc
     3301 tggccaaagg acatcccggc gcccaagcgc gtgataacgc tctgtcgaag gcccgcttcg
     3361 agttccgctg ggaggaccaa ttcaatctct ccctcgatcc ggagaccgcg cagcagttcc
     3421 atgacgaaac gcttcctgac aacgccgcca aagtctcgca tttctgctcg atgtgcggac
     3481 cacacttctg ctccatgaaa atcacgcaag acgtccgaga ttacgccgcc cagctccaag
     3541 tcgacgaaca acgagcgatt cagatcggga tgaacgag
//
LOCUS       DATMTI010000711         4286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_663292, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000711 DATMTI010000000
VERSION     DATMTI010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663292"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..377)
                     /locus_tag="VN039_11430"
     CDS             complement(6..377)
                     /locus_tag="VN039_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46611.1"
                     /translation="MGKIALIVDDSPTMRQMVAFTLTNAGFTVVEAQDGKDAVNKVAG
                     GGKMDIVVTDLNMPEMDGIALIKELRKLSAFRFTPILMLTTESAAEKKQAGKEAGATG
                     WIVKPFNPEVLLKTIAKVLPA"
     gene            complement(433..852)
                     /locus_tag="VN039_11435"
     CDS             complement(433..852)
                     /locus_tag="VN039_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46612.1"
                     /translation="MIPVLKAQMTAVTQETERAAMELLLHLRMLTSSEGEASSKDDSV
                     NLSKVVMAMQFQDITRQKLEHVGLALDRLKAHLQALLKGPGDEEAKKEIAALELVEQH
                     YTMEEERRLHTAALGPDYGEPVPMDIASKEPDSVTLF"
     gene            complement(1063..1365)
                     /locus_tag="VN039_11440"
     CDS             complement(1063..1365)
                     /locus_tag="VN039_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWV46613.1"
                     /translation="MTDERSSSQANQVEGQNARYLAPMGDLTIFEAAEFKESLVKLFV
                     NDGLVSLDLSAVSRVDTAAIQLMLAARKQGRMFVTGISDDLLAKLNQLGFTDSLSE"
     gene            complement(1673..2542)
                     /locus_tag="VN039_11445"
     CDS             complement(1673..2542)
                     /locus_tag="VN039_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWV46614.1"
                     /translation="MIISAAAMAKHKHEEHENHERWLVSYADFITLLFAFFVVMYSIS
                     SVNIGKYRTVSESIKAALNPLANQSGANQIISVGEAKMALQGRNPTDAKEVTIRRIRQ
                     LAQVIKDKQLKDMVAFVTVVQTINGDIVITIPDRLLFNSGEATVKTEALPFLEGLGGV
                     ILEMNRHARVEGHTDDVPIRTAQFPSNWELSAARAVMVVRVLSELYGVPADHLAAAGH
                     ADTRPVTANSDAEQRAKNRRVEVVILEQAPAAPILQSGNESEASEQSAEDMPKNPSQL
                     GPESSGEGRVKMP"
     gene            complement(2608..3378)
                     /locus_tag="VN039_11450"
     CDS             complement(2608..3378)
                     /locus_tag="VN039_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HWV46615.1"
                     /translation="MDIATILGMVIALGSILGGQVLEGGHLSSILQLTAFIIVIGGTV
                     GAICIQNPLPVVIKAIGALSLALSGPRIDNKGTIKLILDLANLSRKQGLLALEGKLKD
                     INDPFMKKGIQLIVDGTEPKVVHEILEIEVEHHEEQGVIAAKVWEAAGGYAPTVGILG
                     AVLGLIHVMENLADPSKLGSGIAVAFVATIYGVGAANLFFLPLANKIKLKLKEEAGGR
                     NLIIMGLVGLAHGENPRLLQEKLESVLPHNERIKEAKK"
     gene            complement(3391..3978)
                     /locus_tag="VN039_11455"
     CDS             complement(3391..3978)
                     /locus_tag="VN039_11455"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HWV46616.1"
                     /translation="MHNQEHKLYGELGALARFVDQAMNAISAASPQIMSSSTQLPAAA
                     SHLSDLSKMTEDGTLEVMRLTEMIQDNHGRIAKELSGIVEVLRAMDCASLAGRLGNVT
                     SVLAQDGKCLTEIMTALSFQDLVAQRVKKLVAILDEVQGKLMELIVVFGLQESCETAR
                     DAGTAGYLLKQLEESKTTAMKQKVADDILAQFGFK"
     gene            complement(4025..>4286)
                     /locus_tag="VN039_11460"
     CDS             complement(4025..>4286)
                     /locus_tag="VN039_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46617.1"
                     /translation="QEALQKLQADTYGFVVSDWNMPVMTGIDMLRAIRADEKLKTTPV
                     LMVTAEAQQSNLVEAVQAGVSNYIVKPFTAETLQEKIAKIFK"
BASE COUNT          855 a         1271 c         1098 g         1062 t
ORIGIN      
        1 cttcgtcatg ccggaagcac cttcgcgatc gttttcaata ggacttcagg attaaaaggc
       61 ttcacgatcc agcccgttgc gccggcttct tttcctgcct gtttcttttc cgccgccgac
      121 tcggtcgtga gcatgaggat cggggtgaat ctgaacgcgc tcaatttccg caactctttg
      181 atgagcgcga tgccgtccat ctccggcata ttcagatcgg tcacgacaat atccattttc
      241 ccgccgcctg cgactttgtt gaccgcgtcc tttccgtctt gcgcctcaac cacggtaaaa
      301 ccggcatttg tcagcgtaaa cgccaccatc tgccgcatgg tgggtgaatc atcgacgatc
      361 agtgcaatct tgcccatggc gacctccggc atcggtgaca gaatcgtatt gaaacagcat
      421 cggacgttcc cgctaaaaga gcgtgacgga atcgggctct ttgctcgcta tgtccatggg
      481 caccggctct ccgtaatccg gccctaacgc cgccgtgtgc aagcgacgtt cctcctccat
      541 agtgtagtgc tgctcaacca gttccagggc ggcaatctct tttttggctt cttcgtctcc
      601 aggccctttc aatagggcct gcaggtgcgc ctttaaccga tccaacgcca aaccgacatg
      661 ctccaacttt tgacgcgtga tgtcttgaaa ctgcatcgcc atcacgacct tggacagatt
      721 gaccgaatca tcttttgacg acgcctctcc ttcggaggac gtcagcattc ggagatgcag
      781 caataattcc atggccgctc tttccgtctc ctgtgtcacg gccgtcatct gagccttcaa
      841 caccgggatc atatgtatgg attcagtcgc aaaggtctcc catgcccgtt gatgttcaag
      901 aagcgctctg agtgtcgttt catcgccggc aagagggcgc tcgagccttt tattgtggtt
      961 tcccgaagag ttcatagccc cgctgacgcg gctcccctcg cacctctcgt cgacccgtcg
     1021 cacttgccgt ccggcaccgc cttggcctcc tcgatatgcg catcactcgc tcagcgagtc
     1081 ggtaaatccc agttgattca atttcgccaa aagatcgtcg gaaatacccg ttacgaacat
     1141 gcgcccttgc ttgcgcgcgg ccagcatcag ctgaatcgcc gccgtgtcca ctcgggaaac
     1201 cgcgctgaga tccagcgaga ccaatccatc gttgacaaac aatttcacta aagattcctt
     1261 aaattccgcc gcctcaaaaa tagtgaggtc gcccatcggc gccaaatatc tggcgttctg
     1321 tccttcaacc tggtttgcct gagacgacga tcgctcgtct gtcatcggtc gtaagctcca
     1381 ttgttgaaat cactggatca tattgtcggc tcgttttgat cgagacttga ggcgcacggc
     1441 gcgatgataa agagatgcgc gacgcgacga agaaggagcg tcaagtttgc gcacgggcgc
     1501 gagtcggtga gcgctcggtg tcctcatttg tgcgcgccgg cgaggtccac gacgcgaaga
     1561 ataatgagcg tcacgtttgt ggacgccgcc gagccggtga ggcggcggtg tcttcgtgag
     1621 acgccagtgt cctcacaaga acgccgttgg agggttttgc aacatcctca acctacggca
     1681 ttttgacccg cccttcgccc gacgactccg gacccagctg cgatggattt ttcggcatat
     1741 cttccgccga ttgctcggat gcctctgatt cattgccgga ctgaagaatc ggagcggcgg
     1801 gggcctgttc gagaatgacg acttcgactc gacgattctt ggccctttgt tcggcatcag
     1861 aattggccgt caccggcctg gtatcagcat gccccgcggc cgccaggtga tctgccggta
     1921 ctccgtagag ttcagacaac acccgcacaa ccatcaccgc ccgtgccgcc gacaattccc
     1981 aattggacgg aaattgtgcc gtccggatgg gaacgtcatc cgtatgccct tcgacgcggg
     2041 catgccggtt catttccagg ataactcccc ctaatccttc gagaaaagga agagcttccg
     2101 ttttgacggt cgcttctcca ctgttaaata gtagtcgatc ggggatcgta atgacgatgt
     2161 ctccattgat cgtctgcacg acggtgacga acgccaccat gtctttcagt tgcttatctt
     2221 taatgacttg ggctaattgg cggatacggc gaattgtgac ttccttggca tctgttggat
     2281 tcctcccctg cagcgccatt tttgcttcgc cgacgctgat tatctggttc gcacccgact
     2341 ggttggcgag tggattcagt gccgccttga tcgattcgct gacggttcga tactttccga
     2401 tgttgaccga ggagatcgag tacatgacga cgaagaacgc aaacagcaag gtaatgaaat
     2461 cggcatagga gaccagccat cgctcgtgat tttcgtgttc ttcgtgttta tgcttggcca
     2521 tggcagcagc gctaatgatc acccgtcatc ggtcattcat ccttcgccat tttttcaatg
     2581 accattgacg gatgactgat gacctcttta cttcttcgct tccttgatcc gttcattatg
     2641 cggaaggacg ctctccaact tttcttgcag caaccgagga ttttcaccat gcgcgagtcc
     2701 gacgagaccc atgatgatca ggttgcggcc tcccgcttcc tctttcaatt tcagcttaat
     2761 cttgttggca agggggagaa agaagaggtt cgccgctccc acgccgtaga tcgtggcgac
     2821 gaatgcgacc gcgatcccgc tgccgagctt cgagggatcg gcgagatttt ccatcacgtg
     2881 aatcaacccc agtaccgcgc cgaggatgcc gacggtcggc gcgtaccccc cggccgcttc
     2941 ccagaccttg gccgctatta cgccttgctc ttcatggtgc tccacttcga tctcaaggat
     3001 ctcatgcacg actttcggct cagtgccgtc gacgatcagc tggatccctt tcttcatgaa
     3061 gggatcgttg atgtctttca gtttcccctc gagcgccaac aacccttgct ttcgcgagag
     3121 attggcgaga tccaagatca gcttgatcgt tcccttattg tcgatgcgag gaccggaaag
     3181 cgccagagat agggcgccga tcgccttgat gacgaccggc agcggatttt ggatgcagat
     3241 cgctccgacc gttcctccga tcacgatgat aaaggcggtc agctgcaaga tcgaactcag
     3301 gtggccgcct tcgagcacct gccccccgag gatggagccc aatgcgatca ccatcccgag
     3361 tattgttgca atatccattg tacggacacc tcatttgaac ccgaattggg ccaagatgtc
     3421 gtcggcgacc ttctgcttca tggctgtcgt tttcgattcc tcgagttgtt tcaataagta
     3481 ccctgctgtg cctgcgtcac gtgccgtctc gcagctttct tgaaggccga acacgacaat
     3541 gagctccatc agcttccctt gcacttcgtc gagaattgca acgagtttct ttacacgttg
     3601 agccaccaga tcttgaaagg atagggccgt catgatctcc gtcagacatt tgccgtcctg
     3661 cgcgagaacg gaggtcacgt tcccgagacg tcccgcaagg ctcgcgcaat ccatcgcccg
     3721 cagaacctcg acgatccccg agagctcctt ggcgattctc ccgtgattat cctgaatcat
     3781 ctccgtcaaa cgcatgactt caagcgtgcc atcctccgtc atcttgctca ggtcactcaa
     3841 atgggaggca gcggccggaa gctgagtact gctggacatg atctgagggc tggccgccga
     3901 gatagcgttc atcgcttggt cgacaaaacg ggccaacgcg ccgagttcac cgtataattt
     3961 atgttcttgg ttgtgcacat cccctctcta ttccggagta agcacggatc tgcttgagca
     4021 cgggttactt gaaaatcttc gcgatctttt cttgcaaggt ctcggctgta aacggcttga
     4081 cgatatagtt gctgacacct gcttgtaccg cctcgaccaa gttgctttgt tgcgcctcgg
     4141 ccgtcaccat caagacgggg gttgttttga gcttctcgtc cgctcggatc gcacggagca
     4201 tgtcgatgcc cgtcatgacc ggcatgttcc aatcggacac gacgaagccg taggtatcag
     4261 cctgaagctt ctgcagtgct tcctgt
//
LOCUS       DATMTI010000712         4068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_834292, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000712 DATMTI010000000
VERSION     DATMTI010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834292"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="VN039_11465"
     CDS             complement(<1..1786)
                     /locus_tag="VN039_11465"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HWV46618.1"
                     /translation="MAKKKASTTNTPAKPTAPPPVSEPATKTKVAAKPAERVTAVEMG
                     ARQREISVSEFFTKNRHLLGFDNPRKALLTCVKEAVDNALDACEEAGILPDVTVRLEV
                     VSNGEPVAPSQATRFRVTVTDNGPGIVRQQIPRIFAKLLYGSKFHRLRMSRGQQGIGI
                     SAAGMYGQLTTGKPVKIISRIGPKAAAHFFEVQIDTKKNEPLVHENKQIDWEQPRGTQ
                     VTLEVEGKYQKGRASVDEWLEQTSIANPHVKLIYQTPEGETKEYPRTYHELPPQPREI
                     KPHPYGIEFGMLLKMLQDTKSHTLSGFLAGDFCRVSPQMADEICKAAKVSPDAKPREL
                     KGPMAETVYKMLQETKIMAPPTNCISPIGEKAILSGLYKQIKGEFYTAVSRLPAVYRG
                     NPFIIEAGLAYGNRPQNQNKPQQPAMPKAEGEHEEEDSELARVIRYANRVPLLYQQSA
                     CSTFKAVLSTTWKNYGLTQSRGALPGGPMVIFVHMASVWVPFTSESKEAIADYDEIQK
                     EITLALRECGRRLGLFVRRRERAASEFRRRNIFELYIEEVVDACNRLKGGKLPKEKLK
                     GQLQKIASSRTGGAKTDEALGKTGGGPEG"
     gene            2102..3892
                     /locus_tag="VN039_11470"
     CDS             2102..3892
                     /locus_tag="VN039_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HWV46619.1"
                     /translation="MYTGLQMIGKYCRWLLLSRDLRVGMVGSALWFFILLPIILLSSS
                     AADISYVYDDASRLRAVIDPASDTAIYAYDSVGNLTGITRQSSSTLAIIQFTPSSSPV
                     GTTVTIYGTGFSATPASNTVKFNGTTATVLTASTTVLTATVPSGATTGSISVTVGATT
                     ATSAASFTVGSGAPTLANFSPAVGNYGATVTLTGTNYDTTLINDRVAFAGAMGAVATA
                     TSTNITVPVPSTAQIGPISVSTVQGTATSSAEFYVAPPGVVSTDVQYTGRVTVDSSTA
                     IASITTANKTGLMVFDGTAGQRVSLGFSGVTVTQFTANVYRPDGVSLLLTIPAFNTSG
                     GSIDVGILPLTGTYTIFLDPVSTYTGNITVTVSTELSGTITPGGAAVPLTISRVGQNA
                     RYTFTGTSGQTISLGITSVSITTLNVSILKPDGSTLVAPTSFGTGGGAIDSQVLPDLW
                     HLCHSGRSILDLYWQCHAHALQYPRCHRHDHHRWRHGDSDLDGSRSTSSLYVYRDGGP
                     MGQSRADQRDDLVEHRLDAEAGWHHVGVDLDQYERWEPGSHHGSAHHGNLHHCRGSHR
                     TRDRHHDTHAHIAAYGEPYAGRCLGAAESE"
     gene            3873..>4068
                     /locus_tag="VN039_11475"
     CDS             3873..>4068
                     /locus_tag="VN039_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46620.1"
                     /translation="MPLNLSKAGQTARYTFSGTSGQWLSLGLTAVTITSSTVTLLNSN
                     GTTLASTSVGTSGGGLEPPST"
BASE COUNT          783 a         1283 c         1105 g          897 t
ORIGIN      
        1 gcccttcagg ccctccgccg gtcttgccaa gcgcctcgtc tgtttttgcg ccacccgtcc
       61 gcgagctcgc aatcttctgg agctgccctt tcaacttttc tttcggcagc ttgcctccct
      121 tgagccggtt acaggcatcc acgacttctt cgatatagag ttcaaaaata ttgcgtcgcc
      181 gaaattcgct ggccgcccgt tcgcgccgac gaacgaagag gcccaaccgc ctgccgcatt
      241 cacgaagtgc cagggtgatt tccttttgaa tctcatcgta gtcggcgatg gcctctttgg
      301 attcgctcgt aaacggcacc cagaccgatg ccatgtggac aaagatcacc atcggtccac
      361 ccggcaatgc tccgcgcgat tgggtcaagc catagttttt ccaggtcgtg ctcaggacgg
      421 ccttgaacgt cgaacaggcc gattgctggt acaacagcgg cactcgattc gcgtaacgga
      481 tcacgcgcgc gagctccgag tcttcttcct cgtgctcgcc ttcggctttc ggcatggccg
      541 gctgctgcgg cttattttga ttctgaggtc tattcccata ggcgagcccc gcttcgatga
      601 tgaacggatt gccgcgatag actgccggta gccgactgac cgccgtgtaa aactcgcctt
      661 tgatctgctt atagagcccc gagaggatcg ccttctcgcc gatcggtgag atgcaattgg
      721 tcggcggcgc catgatcttc gtctcttgca acatcttata gaccgtttct gccatcggtc
      781 ctttcagctc acgaggcttt gcgtcaggcg agaccttcgc cgccttgcag atttcatcgg
      841 ccatctgagg ggagacccgg caaaaatcac cggcgaggaa gccggagagc gtatggctct
      901 tcgtgtcctg caacatcttg agcaacatac cgaactcgat gccataggga tgcggcttga
      961 tctcgcgcgg ttgcggcggc agttcgtgat aggtccgggg atactctttc gtctcgcctt
     1021 cgggcgtctg atagatcagt ttcacgtgag gattcgcgat cgaggtctgc tccagccatt
     1081 catcgaccga ggcacggccc ttctgatact tgccttcgac ttccagcgtg acttgggttc
     1141 ctcgcggctg ctcccagtcg atctgtttgt tctcgtggac cagcggctca ttcttctttg
     1201 tatcgatctg gacttcgaag aaatgcgcgg cggctttggg gccgatccgg gaaataatct
     1261 tgaccggctt cccggtggtg agctgtccgt acatccctgc tgcggagata ccaatgccct
     1321 gttgcccacg gctcatcctc aaccgatgaa acttggaccc atagagcagc ttcgcgaaga
     1381 tccggggaat ctgctggcga acgatgccgg gtccgttgtc ggtgaccgtg acgcgaaacc
     1441 tggttgcttg gctgggtgca accggctcgc cgttcgacac cacctccagc ctgaccgtca
     1501 catcggggag gatcccggcc tcctcacacg catcgagcgc gttgtcgacc gcttctttga
     1561 cgcatgtcag cagcgccttg cgcggattgt cgaagccgag caggtgccgg ttcttcgtaa
     1621 aaaactccga gacggagatt tcccgctgtc gcgcccccat ctcaaccgca gtgacccgtt
     1681 ccgccggctt ggcggcgact ttcgtctttg tggctggttc agagactggt ggaggagcag
     1741 taggtttcgc tggagtattg gtagtagacg ctttcttttt tgccattcac cctctcatga
     1801 aacaaatgtt ctctgttggt accataaaac tttcgcggaa acaatgggaa cacgtcaaac
     1861 gcgccaaagc catacagaac gcgtactcac gcctgctacc acccctcatc aatagttcag
     1921 tccatgccta acaacagcga caacactgag tacccacgct ctatccgacg tcctacccac
     1981 aatgccggac caaatggccc cgtgatacgg ccattttttg ggattgtaca tctctagaaa
     2041 tagcgtaggg tctttcgcgc gagaactctc tttgtaccct tgagtgtgga acccacgtct
     2101 catgtacacc ggattgcaga tgatcggaaa gtactgtcga tggctattgc tgtctcgaga
     2161 cctacgagtg ggaatggtcg gttccgctct gtggtttttc atattgttgc cgatcatcct
     2221 gctgtccagc tctgccgccg acatcagcta cgtctacgac gatgcgagtc ggctgcgcgc
     2281 cgtcattgat ccggcatccg acacggcgat ctacgcctac gatagcgtcg ggaatctgac
     2341 cggcatcacg cgacaatcat cgtctaccct cgcgatcatc cagttcaccc catcatcgag
     2401 tccggtcggc accaccgtga cgatctacgg cacaggcttc agtgccacgc cggcctcgaa
     2461 taccgtgaag tttaacggca caaccgccac cgtccttacg gcgtccacga cggtgttgac
     2521 cgcgacggtc ccgagcggcg cgaccactgg gtccattagc gtcaccgtgg gagccactac
     2581 ggcgaccagt gcagcctcgt ttacggtcgg aagtggtgcc ccgacgctcg cgaatttcag
     2641 ccccgccgtc ggaaactacg gcgccaccgt caccctgacc gggacgaatt atgacacgac
     2701 cctcatcaat gatcgtgtcg cctttgcggg tgccatgggc gccgtggcga ccgccaccag
     2761 caccaacatc acggtacccg tacccagcac ggcacagatc gggccaatct ccgtttccac
     2821 tgtccaaggc acggccacga gtagcgctga gttctatgtg gcgccgccag gggtggtctc
     2881 cacggatgtc caatacaccg ggcgggtcac tgtggacagc tcgacagcca tcgcctccat
     2941 cacgaccgcc aacaagacgg gattgatggt ctttgatggg accgccgggc aacgagtcag
     3001 tctgggattt agtggcgtga ccgtcacgca gttcaccgcg aatgtttatc ggcccgatgg
     3061 cgtctctctg ctgctcacca tcccagcttt taataccagc ggcggcagca tcgatgtggg
     3121 gatcttgccg ctcaccggta cctatacgat cttcctcgat ccggtcagca cctacacggg
     3181 gaacatcacc gtcactgtgt ccacggaact gtctgggacg atcacacctg gtggcgcggc
     3241 cgttccgctc accattagtc gggttggaca gaatgcccgg tataccttca cagggacttc
     3301 cggccagacg attagtctcg ggatcacatc ggtgagtatc acgacgctga acgtgtcgat
     3361 tctcaaaccg gatgggagca ccctcgtggc ccccacctcg ttcgggacag gcggtggagc
     3421 gattgacagt caggtcctcc ccgacctctg gcacctatgc cattctggtc gatccatcct
     3481 tgacctatac tggcaatgtc acgctcacgc tttacaatac ccccgatgtc accggcacga
     3541 tcaccatcga tggcgccacg gcgactccga ccttgacggt tcccggtcaa cgagctcgct
     3601 atacgtttac cgggacggcg ggccaatggg tcaatctcgg gctgaccagc gtgacgatct
     3661 cgtcgagcac cgtctcgatg ctgaagccgg atggcaccac gttggtgtcg acctcgatca
     3721 gtacgagcgg tgggagcctg gatcccatca cggctctgcc caccacggga acctacacca
     3781 ttgtcgtgga tcccacagga ctcgcgaccg gcaccatgac actcacgctc acatcgccgc
     3841 ttacggggag ccttacgctg gacggtgcct cggtgccgct gaatctgagt aaagccggcc
     3901 agacggcgcg ctataccttc agcgggacga gcgggcagtg gctgagttta ggcctgaccg
     3961 ctgtaacgat cacctcgagc accgtgacgc ttctcaactc caatggaacc actctcgcat
     4021 ccacctcggt cgggaccagc ggaggaggac tcgaaccacc gagtacat
//
LOCUS       DATMTI010000713         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_240432, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000713 DATMTI010000000
VERSION     DATMTI010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240432"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VN039_11480"
     CDS             <1..756
                     /locus_tag="VN039_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV46621.1"
                     /translation="VTTIGKPLPLSPLEDNLEADLTPPLEFDKELERMLEESPELGQI
                     ETLVKFERIKVQREQVEPIPNLTVRGDGGVNHEGARRAGPGPWNVANVTAFINVPIFD
                     WNQGTIQQAKAALKNAQYEIARMHLDLRRRLADAFGMYLTSRQYVQALQNIVLPQAET
                     RYRTQLKSYQENRANWRTVLEAQDELFAQRQNHVVRLVQLHIPRVLIKGFLLTGGPPP
                     VGLPGDPFRNANGLTPAPAPDIPGHITATPKPR"
     gene            826..1734
                     /gene="fdhD"
                     /locus_tag="VN039_11485"
     CDS             826..1734
                     /gene="fdhD"
                     /locus_tag="VN039_11485"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWV46622.1"
                     /translation="MRILDDPSRCHPDRSAVLEQRMVDMQVERWSGSDVLQDQDPLAV
                     EEALEIRLVYGATPQRTRKTISVTMRTPGNDLDLAIGFLFAEGLVRCPEDVQSIRHCG
                     PPAGSLRLRNVVRVELRPEAKIDPTGLERNFLSTSSCGVCGKASIEALPALNHTRLPD
                     GFVVAASMIHEFPDMLRRSQMVFDSTGGLHAAALFDRDGRLHDVKEDVGRHNALDKLV
                     GRQFLNGMVPLYDRVLLVSGRVGYELVQKAIAAGIPILAAIGAPSSLAVDLAKDANMT
                     LVGFVRDGRFNIYSASQRICNRTGDS"
BASE COUNT          394 a          529 c          556 g          348 t
ORIGIN      
        1 gtgaccacca tcgggaagcc gcttcccctg agcccgctgg aagacaacct cgaagcagac
       61 ctgacgccgc cgctcgagtt cgacaaagaa ctggagcgga tgctcgagga aagcccggag
      121 ctcggtcaga tcgagaccct ggttaagttc gaacgcatca aggtccagcg ggaacaggtc
      181 gagccgatcc cgaatctcac tgtccgaggg gacggcgggg tgaatcacga aggcgcgcgc
      241 cgggccggac ctgggccctg gaatgtggcg aatgtgacgg cattcatcaa cgtgccgatc
      301 ttcgactgga accagggcac gattcagcaa gccaaggcgg ctctcaagaa tgcgcagtac
      361 gaaatcgcac gcatgcactt ggatctgcgg cgccggctgg ctgatgcatt cgggatgtat
      421 ctgacgtcgc ggcagtacgt gcaggctctg cagaacatcg ttctcccgca ggccgaaaca
      481 cgctacagga cccagctcaa aagctatcaa gagaatcggg cgaactggcg aacggtgctg
      541 gaggcgcagg acgagctctt tgcgcagcga cagaaccatg tcgtgcgcct ggtgcaactg
      601 cacatccccc gcgtgttgat caaggggttt ctcttgaccg gtggaccgcc gcccgtcggc
      661 ttgcccggag atcccttccg gaacgccaac ggcttgacgc cggccccggc tcccgacatc
      721 ccgggtcata tcaccgcgac gccgaagccg cgataagaga gaatgaaaac agccgacacc
      781 gactctctgc ggtgtcatcg aagatgcagc caaggagaac atgccatgag aattctcgat
      841 gacccaagcc gctgtcatcc tgatcggtcc gcggtactcg aacagcgaat ggtcgacatg
      901 caggtggagc ggtggagcgg ctccgatgtg ttgcaagacc aagatccatt ggcggtcgag
      961 gaggcgcttg agattcgtct ggtgtatggt gctacacccc agcgcacacg taagacaatc
     1021 tccgtgacga tgcgcacccc gggcaacgat ctggacttgg ctatcggatt tctctttgcc
     1081 gaaggcctcg tccgttgccc cgaggacgtt cagagcatac ggcactgcgg ccctcccgcg
     1141 ggatcgctcc ggctccggaa cgttgtgcgc gtcgaattgc ggcccgaggc gaagattgac
     1201 ccgactggtc tggaacgaaa tttcctttct acgtcaagct gtggtgtctg cggaaaggct
     1261 tcgatcgagg ccctgccagc cttgaaccac acccgattac cggacggctt cgtcgtggct
     1321 gcttccatga tccacgaatt ccccgacatg ctgcgtcgct cacagatggt gttcgattct
     1381 acgggaggtt tgcatgcagc ggcgctcttt gaccgggacg gacggttgca cgatgtgaaa
     1441 gaagatgtcg ggcggcataa cgctctcgac aaactggtcg gccggcagtt tctcaatggc
     1501 atggtccctt tgtatgaccg agtgctcctc gtgagcggac gcgtgggcta tgagctggtc
     1561 caaaaggcga tcgcagccgg cattccgatc ttggccgcta tcggtgcccc gtcgagcctt
     1621 gcggtggatc ttgcaaagga tgcgaatatg actctggttg ggttcgtgcg ggatggtcgc
     1681 ttcaacatct acagcgcctc tcaacgaatc tgcaacagga ctggtgacag ctgacaacct
     1741 tctcacctca ggggtgaact ctgagcgcag aaaacttccg aacgaaatca atgcgtaatg
     1801 ggactgatca agatggaaga agtaaat
//
LOCUS       DATMTI010000714         1825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_748267, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000714 DATMTI010000000
VERSION     DATMTI010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748267"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VN039_11490"
     CDS             <1..279
                     /locus_tag="VN039_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46623.1"
                     /translation="GGYSFTVGLKGYFGGTDDGKSLIDRQRQDDPPNRAIDLFTAAGN
                     QLYQTAPPSTDNPSNYNDEESCVAHGYVWNDWDSENEYCAATWEEPSG"
     gene            complement(286..1608)
                     /locus_tag="VN039_11495"
     CDS             complement(286..1608)
                     /locus_tag="VN039_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46624.1"
                     /translation="MISQEEQTRRFFETFMETQWWQPGRLQDLQRENLSKLLRHARRY
                     VPFYAERLDTVFRSDGSIDWSNWTRIPILRREDMLQSGEQMRSMAMPGSHGRIRYERT
                     SGSTGDPVTVATSSYAGAALSATIFRAHRWHDFDWSRNFAIRAGRGHPSHPVAEQLAK
                     PLKWGPSWLSQSGGNMLHFETTVDGEEMLSLMREHQAAYLGARPHRAHHMALEAQRLG
                     IELKLDAIWAFGTATSQEARDDCLAAFDARVVSLYSSKEGQLMAIQCPSATHFHINDE
                     TTLLEIVDSDGRACRAGELGRVIVTPIFNFAQPTIRYDHGDMAVAGGSSCVCGRHLGI
                     IERIVGRTTHLFHLPDGRRISPLVPLDIGARIGARFWQVAQTAPLTIEFRYVGGSASP
                     EDRELIVNAIRRGTHHAFEVHLRPMTDLPRTAGGKYLEYVNDYAPGAI"
     gene            complement(1633..>1825)
                     /locus_tag="VN039_11500"
     CDS             complement(1633..>1825)
                     /locus_tag="VN039_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46625.1"
                     /translation="VGANDYEVRFVPQGAAKPDQSLFLTHFRRVFFDDARVVFVPVED
                     IPLTAGGKALEYINEWRRG"
BASE COUNT          391 a          541 c          513 g          380 t
ORIGIN      
        1 ggcggctaca gcttcaccgt cggcctcaag ggctacttcg gcggcaccga cgacggcaaa
       61 tccctcatcg accgccagcg ccaggacgac cccccgaacc gcgccatcga tctgttcacg
      121 gcggcgggaa atcagctgta ccagacggcg ccgccaagta ccgataaccc gagcaactac
      181 aacgatgaag agtcgtgcgt cgcgcacggt tatgtctgga acgactggga ctctgaaaac
      241 gagtactgcg cggcgacttg ggaagaaccg tcgggctgat attggtcaga tagctcctgg
      301 ggcatagtcg ttcacatact cgagatattt gccgcctgcc gtcctgggta ggtctgtcat
      361 cggccgcaaa tgaacctcga atgcgtggtg ggtccctcgg cggattgcgt tgactatgag
      421 ttcccgatct tccggtgaag ccgagccccc gacgtagcga aactcgatcg ttaacggcgc
      481 tgtttgagcc acctgccaaa atcgagcgcc aattcgcgct ccgatgtcca atggtaccag
      541 gggcgatatt cgtcggccgt caggaagatg aaataggtga gtggttcgcc ctacgatcct
      601 ctctatgatt cccaggtgcc ttccacagac gcaggacgag cctccagcaa cggccatatc
      661 cccgtgatca taacgaatgg tcggctgcgc gaagttgaag atgggtgtaa cgatgacccg
      721 gccgagttcg ccagcgcgac atgcgcggcc gtctgaatct actatttcca gcagggtagt
      781 ttcatcgttg atgtgaaagt gtgtagcgga tgggcactga atggccatca gttgcccctc
      841 cttggaactg tacaaggata caactctcgc atcgaaagca gctaaacagt cgtcacgcgc
      901 ctcctgagag gtagccgtcc caaatgccca tatcgcgtcg agcttgagct cgatgccaag
      961 ccgttgggct tcgagcgcca tatggtgcgc tcgatgcgga cgggcaccaa ggtatgcagc
     1021 ttgatgctcc cgcattagcg acagcatctc ctcaccgtct acggttgtct cgaaatgcaa
     1081 catgttgcca cccgactgcg agagccatga gggaccccat ttcagcggtt tagccaactg
     1141 ctcagctact ggatgtgaag ggtggcctcg cccggctcga atggcgaagt tcctggacca
     1201 atcgaagtcg tgccatcggt gagcgcggaa gatggtggcc gacaacgctg caccggcata
     1261 gctcgatgta gcgacggtga ccggatcgcc cgtcgagccg ctcgtccttt catatcgtat
     1321 tcttccatga gagcccggca tcgccatgga acgcatctgc tcgccggatt gcagcatgtc
     1381 ttcgcggcgc aaaatcggaa ttctcgtcca attgctccag tcgattgatc cgtcgcttcg
     1441 gaacaccgta tccagtcgct ctgcatagaa gggaacatag cggcgtgcat ggcggagaag
     1501 cttcgatagg ttttcacgct ggaggtcttg cagtcttcct ggctgccacc actgggtctc
     1561 catgaaggtc tcgaaaaatc gcctggtctg ctcctcctgg ctgatcatcg ccccaacctc
     1621 cagacggcca tctcagccac gacgccactc gttgatatat tcaagcgcct tgccgccagc
     1681 agttaagggg atatcctcaa ccggcacaaa gacgacacgg gcatcgtcga agaacactcg
     1741 tctaaaatga gtcaagaaca gtgattgatc tggctttgct gctccttggg gcacgaagcg
     1801 gacctcgtag tcgttggctc ccacc
//
LOCUS       DATMTI010000715         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_578415, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000715 DATMTI010000000
VERSION     DATMTI010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578415"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1550
                     /locus_tag="VN039_11505"
     CDS             <1..>1550
                     /locus_tag="VN039_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46626.1"
                     /translation="DKYVSGLVLLTGCYFGLVQQTLVTKGAKAAQRVVEMYASWFPNT
                     VVEIQNHNIDHPDGYSDADIVAELIGIADRLGLPVVATQDSHYLKPADKVAHGTMKRM
                     VYRQGDVNEFPGDSFHLASTGWVKQHYTPKQWARSEEGHEYVLGLHKLSLPALDTYKP
                     HVPKMSKTPQTDLEKLCIKRLNELQDKQQLAKNSSDKKPFSTFNRYRDRLIYELGIIK
                     KLGYADYFLHVLDFVNWCRKKEIVIEARGSANGSLVCYLLQVTQIDPLQWKYVTFERF
                     LSPDRQKPPDIDIDIEDIRRPEAVAYIQNKYGAISIGNYSVLGARDDDDKGSVLVSYN
                     AYLRSRMSPAQFNARFGKGIETIEQIRGVSKKDYLGVRRLTKLPVYKSHGVHAAGLLL
                     NGTDLRIDDYVPTMLVASSNTKVSQYTMDDLEALGYLKDDVLGQRTLWVMARCQELMG
                     MQDKTDFSWIPLNDAATCSMLSEGRTNNAVFQFEGYAMATGARSMGIRNTSDCVLAGA
                     LFRPACIDS"
BASE COUNT          354 a          422 c          469 g          305 t
ORIGIN      
        1 gataagtacg tcagtggtct ggtgctgctc actggatgtt attttggact cgtgcagcag
       61 acccttgtta cgaaaggcgc taaggcggct cagcgagtag tcgagatgta tgcgtcctgg
      121 ttccccaaca cggtggtgga aatccagaac cacaacatcg accacccgga tggatactcc
      181 gacgcggata tcgtcgccga gttgatcggc attgcagacc ggctcggttt gcctgtggtt
      241 gcgacgcagg acagccacta cctcaagcca gccgacaagg tggcgcacgg caccatgaag
      301 cgcatggtgt accggcaggg ggatgtgaac gagttccccg gcgacagctt ccacctggcc
      361 tctacagggt gggtgaagca gcactacacg cctaagcagt gggcgcggtc ggaagagggt
      421 cacgagtacg ttctagggct ccacaagctc tctctgcctg ctctggacac ctacaagccg
      481 catgtgccga agatgtccaa gacaccgcag acggacttgg agaagttgtg catcaagcgg
      541 ctcaatgagc tacaagacaa gcagcaactt gcgaagaatt catcggacaa gaagccgttc
      601 tccacgttca accgataccg ggaccggctc atctacgaac tgggcatcat caagaagctc
      661 gggtatgccg actactttct gcatgtgctg gacttcgtga actggtgccg gaaaaaggaa
      721 atcgtcatcg aggcacgcgg ctcggcgaac gggtcgctgg tgtgctacct gctccaggtg
      781 acgcagatcg atccgctgca atggaagtac gtcacgtttg agcggttcct ttccccggac
      841 cggcagaagc cgccggacat cgatatcgac attgaagaca tccgtcgtcc ggaagctgtg
      901 gcgtacatcc agaacaagta cggcgcgata tcgatcggga actactcggt gctgggagcg
      961 cgggatgacg acgacaaggg ttcggtcctg gtctcgtata acgcctatct tcgtagccgg
     1021 atgtctccgg cgcagttcaa cgccaggttc gggaagggca tcgagaccat cgagcagatt
     1081 cgcggggtca gcaagaaaga ctacctagga gttcgaaggc tgacgaagct gccggtgtac
     1141 aagtcgcacg gtgtgcatgc ggccggtctg ctgctcaacg gaacagacct gcggatcgac
     1201 gattatgttc cgaccatgct ggtagccagt agcaacacca aagtcagcca gtacacgatg
     1261 gacgacctgg aagcgcttgg gtacctgaaa gacgatgtac tcggacagcg gactttgtgg
     1321 gtcatggcac gttgccagga actcatgggc atgcaggaca agactgactt ctcctggatt
     1381 ccgctgaacg acgcggcaac ctgctccatg ctttcggagg ggaggacgaa caatgccgtc
     1441 ttccagtttg agggatacgc catggcgacg ggtgcacggt ctatgggtat ccgaaacacc
     1501 tccgactgtg tactggccgg agctttattc cgcccagcct gtattgattc
//
LOCUS       DATMTI010000716         1602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_281142, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000716 DATMTI010000000
VERSION     DATMTI010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281142"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VN039_11510"
     CDS             <1..389
                     /locus_tag="VN039_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46627.1"
                     /translation="IKAEADIQIAERELRALTDNEQSDLSAIETKLKQSEDLQVGLRM
                     TSIKTRRDVMGLLTPEQRAKEKSEHDKAMQQHKGYGTPHGGAMPYGMNPHGANPHGAN
                     PHGADPHGKNPHEAVPPTAPSNMSVQ"
     gene            407..>1602
                     /locus_tag="VN039_11515"
     CDS             407..>1602
                     /locus_tag="VN039_11515"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWV46628.1"
                     /translation="MKKEVQLQSHDLLVGASRAPARAMLKAVGFTDDDLSKPLVGVAN
                     TWIEVMPCNFHLRRLSERVKAGIRAAGGTPVEYNTIAVSDGISMGTEGMKASLISREV
                     IADSIELVARGHLFDAVVALSGCDKTIPGTVMALARLNMPSLMLYGGSIMPGQFQGHD
                     VTIQDVFEAVGKHASGKMTDAELKDLEDHACPGPGACGGQFTANTMAIAFEFLGISPM
                     GRNGVPAMDGRKDDVAFECGKMVMELVKQDLRPRQIITRKSLENAIAAVATTGGSTNA
                     VLHLLAIARESGIKLSIDDFDKMNRKVPLLADLKPGGRFAAADLYAAGGTILVAKRLL
                     DAGLLHGNQPTVTGRTIGEEAKDAVESPGQQVLRPLSHPIKPTGGLVILKGNLAPEGC
                     VVKVAG"
BASE COUNT          370 a          467 c          467 g          298 t
ORIGIN      
        1 gtatcaaggc tgaagccgat atccaaatcg cggagcgcga actgagagca ctgacggata
       61 atgagcagtc cgatctctcc gcgatcgaaa ccaagctgaa acaaagtgaa gatctgcaag
      121 tcggactgcg gatgacttcc atcaagacac gccgcgatgt aatgggctta ctgaccccgg
      181 aacaacgtgc gaaggaaaag tccgagcacg acaaggcgat gcagcagcac aaagggtatg
      241 gcactcccca tggaggcgcg atgccctatg ggatgaatcc tcatggtgcc aatcctcacg
      301 gtgcgaatcc acatggcgcc gatccgcacg gcaagaatcc ccatgaggct gtccctccta
      361 ccgcacccag taacatgtct gtccagtaag ggaaccacga agaccgatga aaaaagaagt
      421 gcagctccag agtcatgatc ttctggtcgg ggccagccgt gccccggcga gggccatgtt
      481 aaaagccgtc ggctttacgg acgacgacct gtcgaaacct ctcgtcggcg tggccaatac
      541 ctggatcgag gtcatgccgt gtaattttca tttacggcgg ctgtccgaac gagtgaaagc
      601 cgggatcaga gcggccggcg gtactcccgt tgaatacaac acgatcgcgg tgtccgatgg
      661 gatctcgatg ggcaccgaag ggatgaaagc ttccctcatc agccgtgagg tcatcgccga
      721 ttcgatcgag cttgtcgcgc gtggacactt gtttgatgcc gtcgtcgccc tgtccggctg
      781 cgataaaacg attcccggaa ccgtcatggc cctcgcccga ttgaacatgc cgtcgctcat
      841 gctctatggc ggctccatca tgccgggaca gtttcaggga catgacgtga ccattcagga
      901 tgtcttcgaa gcagtcggca aacatgcgtc gggaaagatg accgacgccg agctgaagga
      961 cttggaggat cacgcgtgcc ccgggcctgg agcttgtgga ggccagttca ccgccaatac
     1021 catggccata gcgtttgagt tcctcggcat ttcaccgatg ggacgcaatg gggttcccgc
     1081 catggacggt cggaaagacg atgtcgcctt tgaatgcggc aagatggtga tggaactcgt
     1141 aaaacaagat ctgcgtccgc gtcagattat tacccgaaag tcgttggaga acgccatcgc
     1201 cgccgtcgcc acgaccgggg gctcgacaaa cgccgtgctg catctccttg ccatcgcccg
     1261 tgagtccggg atcaaactga gcatcgacga cttcgacaag atgaatcgca aggtcccctt
     1321 gctggccgac ctcaagccgg gaggccgctt cgcagcggca gacctctatg ccgccggcgg
     1381 gacaatctta gtcgccaaac gattactcga tgccggcctg cttcacggca atcagcctac
     1441 cgtcacaggc cgaacgatcg gcgaagaagc caaggatgcc gtggagagtc ctgggcaaca
     1501 agtcttgcgt cccctctccc accccatcaa accgacaggc ggccttgtca ttctgaaggg
     1561 caatttggcg ccggaaggtt gtgtggtgaa ggtcgccggc ca
//
LOCUS       DATMTI010000717         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_409422, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000717 DATMTI010000000
VERSION     DATMTI010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409422"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VN039_11520"
     CDS             <1..308
                     /locus_tag="VN039_11520"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HWV46629.1"
                     /translation="TWGRKFVDWEISSSLRNDSVNRRSGLLVIPLPSRKNSAHLPDRI
                     KDNWVANDEAASYAHYKSYPTRLTSFRADVEAAFQARASKGGLVDNSRELRQRNATS"
     gene            328..1491
                     /locus_tag="VN039_11525"
     CDS             328..1491
                     /locus_tag="VN039_11525"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HWV46630.1"
                     /translation="MTTTYGIRVIHKNRQEDLETLRQAVAQEVSKLGLHRSVAVRVTE
                     SGPPGSTPYVCVYLGDESTCVDPSIVIELAQELAAGTTVFPVVPDLTLFSTLVPAEIS
                     FANGIVWRDETSLRHLVRVLLEDLGIEDRQRRVFISHRRDDGLGAAEQLHDKLTHSGF
                     RPFIDRFAIREGADFQAEIADALEDHAFLLLLETPLAHTSDWVYDEVDYALSHTMGVL
                     IARWPGDVQEVPGSPGVPRLPIGQDELQIDEHGFEIFTTSGLDRLVEEIEAAHAFGLV
                     RRRRMLVRSIEESALASGVDSCIALRDWRLLIETPKGKRIVGTTPRLPTAADLQNVDE
                     ASADAGADLPGLLVHSARLLSTPLKKHLEWVSGSRQLSMIPENAIGGWWSHAN"
     gene            1502..2149
                     /locus_tag="VN039_11530"
     CDS             1502..2149
                     /locus_tag="VN039_11530"
                     /inference="COORDINATES: protein motif:HMM:NF036730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46631.1"
                     /translation="MRTTRPLNRGAVFLSGSIPDRNRWEGNFDPLEITDAVVAFARAC
                     LTRKLSIVTAAHPTLAPLLLYVAAEFPVSDEPQVVIYQSLLFESVLPAATRRFEARGI
                     GELIWTEAAPGEEPVPGHWDASLSEMRERMLVETDPWAAAFIGGMVGIEDEFARFAEL
                     FPGRPMYAVRRPGGEASCLSSKHHLPGLDDADQAVSYPTLWNRFLDGVGPPEDHD"
BASE COUNT          493 a          633 c          737 g          543 t
ORIGIN      
        1 gtacttgggg tcgaaagttt gtcgactggg agatctcatc cagtcttcga aatgactccg
       61 tcaaccggcg gagtggactc ctcgtcattc cgcttccttc gaggaagaat tctgcccatc
      121 ttcctgatcg catcaaagac aactgggtcg cgaacgatga ggcagcttcg tacgcgcatt
      181 acaagagcta tccgacgaga ttaacttcgt ttcgggccga cgttgaggcc gcgtttcaag
      241 ctcgggcttc taagggcggc ttagtcgaca actccaggga acttcgacag cgaaacgcta
      301 catcctgaat cagagagtgg ctccgatatg accacgacat atgggattcg agtgattcac
      361 aagaatcgtc aagaagactt ggagacgttg cgccaagctg tcgctcagga ggtctcaaaa
      421 ttgggccttc accggtccgt tgcggtcagg gtcactgaga gcggcccgcc ggggagcact
      481 ccttatgtct gcgtctatct gggggacgaa agtacgtgcg ttgacccctc aatcgtgata
      541 gagctggcgc aagaactagc tgcgggaacg acggtatttc cggtggttcc tgatttgaca
      601 ctattttcta cgttggtccc ggcggagatt tccttcgcga atggcatcgt ttggagagat
      661 gagacctccc ttcgccacct cgtgcgtgta ttactcgagg atctcggtat agaagaccgc
      721 caacgacgag tgtttattag tcatcgtcgc gatgacgggc tcggcgcagc agagcagttg
      781 cacgacaagc tgacacattc tggctttcgg cccttcatcg accggttcgc tatccgcgag
      841 ggcgctgact ttcaggccga gatcgccgac gcacttgagg accacgcgtt tctactcctt
      901 cttgaaaccc cacttgcaca cacttcggac tgggtttatg acgaagttga ctacgctctc
      961 agccacacaa tgggagtgct gattgcccgg tggccaggtg atgtgcagga ggtcccggga
     1021 agccctggag tgcctcggct gccaattggt caggacgaat tgcaaattga cgagcacggt
     1081 tttgagatat tcaccacctc gggattggac cgcctagttg aagaaatcga ggcagcgcac
     1141 gcttttggat tggttcgacg gcgccgaatg cttgtgagga gtattgagga atccgcgctt
     1201 gcttctggag tcgactcatg catcgccttg agggactggc gactgctcat tgaaacgccg
     1261 aagggcaaga ggatagtggg aaccactcct cgattgccca cggcagcaga ccttcaaaat
     1321 gtcgatgaag cgtcagcgga tgccggtgcg gacctgccgg gattgctggt tcacagcgct
     1381 cggttgctct ccaccccact gaagaagcat cttgaatggg tgtcaggcag tcggcagcta
     1441 agcatgattc cagaaaatgc gattggtggg tggtggtctc atgccaattg atttcgagtc
     1501 catgcgcaca acaaggccct tgaatcgcgg cgccgtcttt ctgtccggga gcattccgga
     1561 cagaaaccgc tgggaaggca actttgatcc acttgagata acggatgctg tcgtcgcatt
     1621 tgcgagggca tgcctcacga ggaagctctc tatcgtgacc gcggcccatc cgacccttgc
     1681 acccctcttg ctgtacgttg cagcggagtt tccagttagc gatgaaccgc aagttgtcat
     1741 ctatcagtct ctgctctttg agtccgtgct tcctgccgcg acgcgtagat tcgaagctag
     1801 aggcatcggc gagctgattt ggaccgaagc ggcaccgggc gaggaaccgg tgccgggcca
     1861 ttgggacgcg agtctgagtg agatgcgtga gcgcatgctg gtggaaactg atccttgggc
     1921 ggcggcgttc atcggaggaa tggtcggtat cgaggacgag tttgcccggt ttgccgaact
     1981 ctttcctggt cgcccaatgt atgcggttcg ccgtcctggc ggggaagctt cgtgtctgag
     2041 ttctaagcac catcttcctg gcctggatga cgctgatcag gctgtgtcat acccaacact
     2101 gtggaaccgc ttcctcgacg gggtgggccc gcccgaagat cacgattgaa atgcgggtcg
     2161 ggcagattgt gaggacgaca gcgactttcg agaatgggga gggggattga agccgaacgc
     2221 gttgcaatgc tcgtcaagga gtctcgtacc agaagagtcc gagacgggag agcgcatcga
     2281 cgagttctgg ggtgtcggcg gcgacgggcg ctttccactc tggggcgatg aggttactgt
     2341 gccgtttact ctcgtcgggt gagcgttcga acagagccca catgcggatc tcgtcgccga
     2401 ggcggg
//
LOCUS       DATMTI010000718         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_111477, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000718 DATMTI010000000
VERSION     DATMTI010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111477"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3037)
                     /locus_tag="VN039_11535"
     CDS             complement(<1..3037)
                     /locus_tag="VN039_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HWV46632.1"
                     /translation="MVQSEIEYVAPRTPTEQLIADIWIELLQVDRVGIHDDFFDLGGH
                     SLLATQLMSRIGTICQVKLSLRTIFEVPILADMAAAVDEIHAEATTVSDQKVMPSETF
                     PLSFAQQRLWFLDQLDDHGSAYSIPLAVRLSGKLNRGALRSALNDVVARHDALRACFE
                     SNEGTPAQRIRSKVELKIELNDLRHLSQGEREAAMQKLMCDEALLPFDLCADPLIRGS
                     LLQMEDQEHLLLLTMHHIVSDGWSMGILIREFAALYASHALGLPSALPELPMRYVDFA
                     NWQRQWLSGEVLDQQLDYWKTQLADSPSLLSLPADRPRPSAQSQSGATLPYVVSAELC
                     TKLRALSRETKSTLFMTLCAAFNVLLARYSGQNDICIGTPIAGRNREDIEGLIGFFVN
                     TLVLRTKVDSSQGFSVLLKQVKTLTLDAYTHQDVPFEQLVEVLQPERDSSYTPLFQTM
                     LVLQNAPMTKLALPGLQLELVPTGGATAKFDLTLDLIEAKDGLLGHFEYNTDLFDAST
                     IERMADHFTQLLHAIVADPTCPVGDLTMLDDNERHRMLYEWNDTGLASPLHMQTIHEM
                     FEAQVARTPDNVAVVYEGSTLSYVELNKRANQLAHHLRTLGVGPDVLVGICMARTPDM
                     IVCMLGVLKAGGAYVPLDPDYPPARIATMLADAQPRVLLTERNLQQNLPDVVGTQVFC
                     IDAEDSLLAECCANNPAHVTGADHLAYVIYTSGSTGKPKGVAIRHGNTATFIHWALAT
                     FDSNSLDKVLVSTSICFDLSIFEIFVTLSQGGSAWLAKNILEFSENSARMPVTLINTV
                     PSAMAEVYRSKTLPPSVKVINLAGEALANSLAQALYQQASVEKIFNLYGPSEDTTYST
                     FALVEKGSTATVPIGRPIANTQTYILDNRCNPVPIGVAGELFLAGDGLARGYLNQPGL
                     TAEKFISNPFSVTPGARMYRTGDLVRYRVDGSIEYLGRIDHQVKIRGFRIELGEIEAR
                     ITEHPAVGEAVVLVREDQPGDKRLVAYLVAQAGHALPDEA"
BASE COUNT          691 a          863 c          820 g          670 t
ORIGIN      
        1 gtgcttcgtc aggcaacgca tgcccggctt gcgcgaccag ataggccacc agacgcttgt
       61 caccgggttg gtcttcacgt accaggacca ccgcttcgcc caccgcagga tgttcggtga
      121 tgcgcgcctc gatttcaccc agctcaatgc ggaaaccgcg aatcttgact tgatggtcga
      181 tccgtccgag gtattcgata ctgccgtcca cacgatagcg aacaagatcc ccggtgcgat
      241 acatgcgagc accaggcgtg acactgaatg gattggagat gaacttctcg gcagtcaagc
      301 caggttggtt caagtagcct cgcgcaagcc cgtcgccagc aaggaacaat tcaccagcga
      361 ccccgatggg taccgggttg catcggttgt cgagaatata ggtctgtgta ttggcaattg
      421 gtcgcccgat gggaaccgtc gcggtcgaac ctttctccac cagagcaaag gtggaatacg
      481 tcgtatcctc cgatgggcca taaagattga aaatcttctc gaccgatgcc tgctgataaa
      541 gcgcctgtgc caacgaattg gcaagcgctt caccagccag gttgatgacc ttgactgatg
      601 gcggaagtgt cttgcttcga tatacctccg ccatcgcgga tggcacggta ttgatcaggg
      661 taaccggcat cctggccgaa ttctccgaga actcgaggat gttcttggct aaccaggccg
      721 aaccaccctg gcttaatgtc acgaaaatct cgaagatcga caagtcgaaa caaatcgatg
      781 tggataccag caccttgtcg agactgttgc tatcgaacgt ggccagggcc caatggatga
      841 atgtggctgt atttccgtgt cgaatcgcca ctcctttcgg cttgccggtc gaaccggagg
      901 tatagatcac ataggcgaga tgatcggcgc cggtgacatg tgcaggatta tttgcacaac
      961 actcagccag cagtgagtct tctgcatcaa tgcaaaacac ctgtgttccg accacgtctg
     1021 gcaaattctg ttgcaagttt cgctcggtca gaagcactct tggctgtgca tccgccaaca
     1081 tcgtggcaat gcgtgccggc ggatagtctg gatcgagcgg cacgtaggca ccgccagctt
     1141 tcagcacgcc aagcatgcaa acaatcatgt ctggcgttcg tgccatgcag ataccgacca
     1201 gcacatccgg acccacaccc agcgttctca gatgatgagc caactggttc gctcgcttgt
     1261 tcagctcgac ataactgagc gtggatcctt cgtataccac cgcaacgttg tccggagtgc
     1321 gtgccacctg cgcctcgaac atttcgtgaa tggtttgcat gtgaagaggc gatgcgagac
     1381 cggtatcgtt ccattcgtac aacatgcggt ggcgttcgtt gtcatcgagc atggtcagat
     1441 cgccaacggg gcaagtcgga tcagccacga tggcatgcag cagttgcgtg aaatgatcag
     1501 ccatacgctc gatagtgctg gcgtcaaaca gatcggtgtt gtactcaaaa tgccccagca
     1561 agccatcctt cgcttcaatc agatccaggg tcaggtcgaa tttggcggta gctccgcccg
     1621 tcgggaccag ctccagttgc aatcctggca aggccagctt ggtcatgggc gcgttctgca
     1681 gcacaagcat ggtctgaaac aggggcgtat aacttgaatc ccgttctggt tgcagtacct
     1741 cgaccaattg ctcaaacggc acatcctggt gtgtataggc atcgagcgta agggtcttta
     1801 cctgtttcag taatacactg aacccctgac tcgaatccac cttcgtgcgc agcaccaggg
     1861 tgttgacaaa gaaacctatc aggccctcga tatcttcacg attgcggccg gcaattggcg
     1921 tgccaatgca gatatcattc tgtcccgaat aacgcgccag cagcacgttg aaggcggcac
     1981 acaacgtcat gaacaacgtg ctttttgtct cccggctcaa ggcccgcagt ttcgtgcaga
     2041 gttccgctga aaccacgtat ggcaacgtgg caccggattg actttgcgcg gacggacgcg
     2101 ggcggtcggc aggcaggctc agcaagctcg gactgtccgc caattgtgtc ttccagtaat
     2161 caagttgctg atccagcacc tcaccgctca gccactgcct ctgccagttg gcaaagtcga
     2221 cataacgcat cggcaactct ggcaacgccg acggcaggcc gagcgcatgg ctagcgtaca
     2281 gcgctgcgaa ctcccgtatc agaatgccca ttgaccagcc atccgagacg atgtgatgca
     2341 tggtcagcag cagcagatgt tcctgatctt ccatttgcag aagactgcct cgaatcaatg
     2401 gatcggcgca caggtcaaat ggcaagagtg cttcgtcgca catcagcttc tgcattgcag
     2461 cttcacgctc accttgagaa aggtggcgca gatcattgag ttcgatcttc aactcgactt
     2521 tcgatctgat tcgctgcgca ggcgtgcctt cgttgctttc aaaacaggca cggagtgcat
     2581 catgacgagc gacaacatca ttgagtgcgc tgcggagtgc tcctcgattg agcttgccgc
     2641 tcaatcgaac tgccagcggt atgctgtagg ctgagccatg gtcgtccagt tgatccagaa
     2701 accacaggcg ctgctgcgca aaggacaatg gaaaagtttc cgaaggcatt accttctgat
     2761 ccgataccgt tgtcgcttcg gcgtgaattt cgtcgaccgc cgcagccatg tccgccagga
     2821 tcggcacctc gaaaatcgtg cgcaacgata atttgacctg acatatcgtc ccgatgcgtg
     2881 acatcaattg tgttgccagc agtgaatggc cacccaagtc gaagaaatcg tcatggatgc
     2941 cgacccgatc gacctgcaga agttcgatcc agatatccgc gatgagttgt tcagtgggcg
     3001 tgcgcggcgc cacgtactcg atttcgctct gcaccatgtc cggc
//
LOCUS       DATMTI010000719         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_705126, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000719 DATMTI010000000
VERSION     DATMTI010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705126"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1166..>1609
                     /locus_tag="VN039_11540"
     CDS             1166..>1609
                     /locus_tag="VN039_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV46633.1"
                     /translation="MSIGDNRAVVITGASTGIGAACALHLDRVGFAVFAGVRKSEDGV
                     ALQKSGSDRLVPMELDVTDLTTIQKSHAVVLEATRERGLFGLINNAGIAVVGPLEAVP
                     ISDLRQQLEVNVIGQVAVIQAFLPLVRQARGRIVNMGSIAGLSTMP"
BASE COUNT          413 a          401 c          420 g          375 t
ORIGIN      
        1 gcgcccccta cgcctccaaa tcgaaagtga caccccaccg gtatagcgtc cagtaatcaa
       61 caagcaataa aaaacccctg aagccgtgcg actccagggg attcgatttc tcgataggct
      121 aaataactgc tggatgccta gctagcagag cagtttcatg ttacgccgtc agaatatagc
      181 ggttttgggc ttacgcgtgg taacgctggc atatcattca catggccttt gtgaaagtgt
      241 ctcgctcaag aaatgtggaa ggagaggagc gcgacttgga ggaggaagat ttcgtcagac
      301 tcacgatcca ctgttggcat tttggcaagg cctaatctcc agccgcttcc ttgacacccc
      361 tttccacatc ggcatagcat tagttgccat atacgttcct gcagaagaag atcttaatta
      421 gacatcctgt atggaatctg aaagagggaa tgcacggcca tgaaaacgct tatcataatt
      481 gctcgggatt ccatgctcaa ggagttggag gagcttttgc gtaataatgg tgtcatggca
      541 tataccaccc ttagcaacgt atcaggtcaa ggtgtaaccg gtaaagtgta tgggaccttc
      601 cttaaccccg ctattaatgc cgtcatttgt gcagttctta caacagacga agcagacaag
      661 gcaatcatag cccttaagac gtttcataca aggcgaaagg agactgcgcc ccgggatcaa
      721 cctattccac ttaaagtatt ctcgtggttt tgtgacgagc acgtatagcg agattgattt
      781 acaactaata caacatgctt ttgcacctgc catccagagt gacttgcgga aggatattgt
      841 cgaatggatg aaaccactgc ctccactgtg cccgtaattg tgcccgcgag cggctatata
      901 gcaccattct ccacccccca gcaaccaatg aggcaataaa aaaccccttg taaccgttga
      961 agattacaag gggctcttgt tatctggttg gcttatgagt ccgctgctct gccaactgag
     1021 ctacaccgcc actcagaaga aaccaaatga taactgaaga tcgaggccgg tgtccatcga
     1081 gtgagttgga aatagctgcg ccatgtatgg atgaagtcgc gtgaaacctg gtatggtcct
     1141 ccggtcgcga ttcaaaagga gcaagatgtc gatcggggac aatcgcgcgg tggtcatcac
     1201 gggagcgtct actggaattg gggcggcctg cgcgctgcat cttgatcgag tagggttcgc
     1261 cgtttttgca ggagtgcgaa aatcggaaga cggcgtggct cttcaaaaat ccggttccga
     1321 tcggctggtc ccgatggaac tcgatgtgac ggacctcaca accattcaga aatcccacgc
     1381 agtagtgtta gaagcgacga gagagcgcgg attatttgga ctcattaata atgctggaat
     1441 tgcggtggtg ggaccgcttg aagccgtacc gatctccgac ctccgccagc agctggaggt
     1501 caacgtcatc ggacaggtgg cagtcatcca ggcatttttg cccttggtcc ggcaggcacg
     1561 gggacggatc gtcaacatgg gttccatcgc aggactctcg accatgccg
//
LOCUS       DATMTI010000720         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_705131, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000720 DATMTI010000000
VERSION     DATMTI010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705131"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VN039_11545"
     CDS             <1..538
                     /locus_tag="VN039_11545"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWV46634.1"
                     /translation="RLFPYAGLVSAFVIFTGCALTGDQAEPLPSGAPSITYEQVKAAP
                     ESYNGQPVTFGGKVLGARRLKEGTRIEILQLPLTGSLRPTVDLGKSQGRFVALQKDFL
                     DPATVPPGTFVTVTGEMAGSVTLPLDETEYTYPLVRINNLRVWTEDDEESPLIRRPIG
                     PYWSPYWSPYWHSWPYYW"
     gene            857..1570
                     /locus_tag="VN039_11550"
     CDS             857..1570
                     /locus_tag="VN039_11550"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWV46635.1"
                     /translation="MMIRNDRAVKGKPSNLSSSIVAALKEQIIHWQYPPEHRLTEAEL
                     CEKFNVSRSPVREALRVLASDGFVKKLPNRAYVVRQYNLGEIEELYDLRLALELYTVE
                     RLATKDPLHRNDKEAISKLKSTWIELLNGPAKKADELAKLDTLFHETLAHVLGNKPLL
                     QHLRTINERLMLFRMIDFDKSDRAESTCRQHLKILKCITAGDARGARAVMEQNIGEGK
                     NNVHTAIKDALAKAYSLRT"
     gene            1610..>2532
                     /locus_tag="VN039_11555"
     CDS             1610..>2532
                     /locus_tag="VN039_11555"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HWV46636.1"
                     /translation="MHFQALSVGIRVPTRHFNGTVHSVFRQACNLWLEPGTLLTLLPC
                     QTGNLPHGIRLNTFSHSVFLHVLRVGQPVACRGGILRSDGPDFSIDLRPAWPWHIDLK
                     GLRIDLCGPTQAQSWAVAWSELKMHCHKHGMSENLGALFPSEGRLAMSAVSEILRRQS
                     ASTIPLLLQATCRLRLSEAIISMRSLIGLGYGLTPSGDDFLVGYLTGLWCTAGANPSR
                     MRFLLALGSELSEAARNTNEISGAYLQSAATGHVSEPIAKLAQQLSQADTMSSVRAVT
                     QTALQVGHTSGSDGVLGMLLGCLAWQSLPPHL"
BASE COUNT          561 a          757 c          661 g          553 t
ORIGIN      
        1 gcgcctattc ccctatgcag gcctcgtctc cgcattcgtc atcttcacag gctgtgcttt
       61 aaccggtgat caggccgagc cgctaccttc cggcgcacct tccatcacct atgagcaggt
      121 caaagccgct cccgaatctt acaacggaca gccggtcaca ttcgggggga aagtgctggg
      181 agcacgtcgg ctcaaagagg gaaccagaat cgagatactc caattgccgt tgaccggctc
      241 ccttcgcccg acagtggacc tcggcaagtc ccaaggtcgg ttcgtggccc tacagaagga
      301 ctttctcgac cctgcgacgg ttccgcccgg tacgtttgtg accgtaaccg gagagatggc
      361 cggctccgtc accttgccgc tcgatgaaac ggaatacacc tacccgctcg tgcgcatcaa
      421 caatctgcgc gtgtggaccg aagacgacga ggaatcacct ctcattcgcc gtcccatcgg
      481 cccctattgg agcccttact ggtcgccgta ctggcactct tggccctact attggtaagc
      541 tggccgcgcg ggactctcgg caccgtgacg ggcaagtctc aagccgactc ttcattctct
      601 ctctcacccg ccttgtccat caccgtaaac gatgtccacg tgtccacctt actcccttat
      661 caccctctcg actctcgaca tcttcatttc tgcgccggcg accggcgata tgccatccat
      721 gacacattcg acttggtacg cttaattgta tacaatccat ggagttgccg ccgctgaaga
      781 tgaaaccgac ttgtctgccc gcacatgacg gagcgtccat cataaacttt tctgaagctc
      841 ggggtcccga aagaacatga tgattcgaaa tgaccgagcc gtgaaaggaa aaccatccaa
      901 ccttagttcc tcgattgtcg ccgcactgaa ggaacagatc attcattggc agtacccacc
      961 tgaacatcgg ctgactgaag ctgagctgtg cgagaaattc aacgtgagtc gaagtccggt
     1021 gcgggaagcg ctccgtgtac tggcatccga tgggttcgtg aaaaaattgc ccaatcgggc
     1081 ctatgtcgtc agacaataca acctcggtga gatcgaggag ctatacgacc ttcgcttggc
     1141 gttggaactc tacaccgtcg aacgcttggc gacaaaggac cctctccacc ggaacgataa
     1201 ggaggccatc agtaaactca agagcacatg gatcgagctg ttgaatggac ctgccaagaa
     1261 ggccgatgaa ctcgccaaac tagacacctt gtttcacgag acactcgccc atgtcttagg
     1321 aaacaaaccg ttattgcagc acctccgcac catcaatgaa cggttgatgc tttttcgaat
     1381 gatcgatttc gacaagtcgg atcgagcgga aagcacatgc cgtcagcatc tcaaaatcct
     1441 gaagtgtata accgccggag atgctcgggg cgcgcgggct gtgatggaac aaaatattgg
     1501 agaaggaaag aacaacgttc atacggcgat taaagacgcg ctggccaaag cgtactcatt
     1561 gagaacttag gaaggttttc tcatctcacc gggcgatgga gtgcccatca tgcatttcca
     1621 ggccctttca gtcggcatcc gggtgccgac ccgtcacttc aacggaaccg tgcacagcgt
     1681 gttccgccag gcctgcaacc tatggctgga gcctggtacg ctgctgaccc tccttccatg
     1741 ccaaacaggc aatctccctc acggcattcg cttaaacacc ttctctcatt ctgtcttcct
     1801 gcatgtcctt cgagtcggac aaccggtcgc gtgtcgggga ggaatactcc gcagcgacgg
     1861 accggatttt tccatcgatc tgcgacctgc atggccctgg cacatcgatc tcaaagggtt
     1921 gcgcatcgat ctttgcggac ccacccaggc tcaatcctgg gcggttgcct ggtctgaatt
     1981 gaaaatgcat tgccataaac atggcatgtc ggagaatctg ggggccttgt ttccgtcaga
     2041 agggcggctt gccatgtcgg cggtctcgga gattcttcgc cgacagagtg cttccactat
     2101 cccgttgctc ctacaggcga catgtcgcct ccgattaagc gaggccataa tctccatgag
     2161 atcactgatc ggacttgggt acggcctgac gccatcgggc gatgatttcc tcgtgggcta
     2221 cttgaccgga ttgtggtgca cggcaggggc caacccatct cggatgcgat ttctgctggc
     2281 cttaggctct gaactgtcgg aagcggcgcg gaataccaat gagatcagcg gcgcttatct
     2341 ccaatccgca gccacgggcc atgtctctga accaatcgcg aaattggcgc agcagctgag
     2401 ccaggcggac actatgagca gcgtcagggc agtcacgcaa accgctctac aagtcggcca
     2461 tacgtccggc agcgacggcg tgttagggat gttgttgggc tgccttgctt ggcagagcct
     2521 gcctcctcat ct
//
LOCUS       DATMTI010000721         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_409589, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000721 DATMTI010000000
VERSION     DATMTI010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409589"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2021
                     /locus_tag="VN039_11560"
     CDS             <1..>2021
                     /locus_tag="VN039_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HWV46637.1"
                     /translation="DRITKYGYDALGDLTSTTSAYGTSLARIDAAKVYDIGSSTSTGT
                     LQYATDAKGNKTSYAYDGFNRLIKTCYPTPSNGGTTNTSDCDQTAYRSVLVDNTTTQA
                     TSLVDHLVLRDGQTISFVYDFLGRVSAKSGAVSESYAYDNYNDVTTHVNNTTGGASAS
                     ETYGYNALGWLLSDTQPIGAVSYGYDAYGRRNKLTYPDGFYVTYQYWNDDSGWYIQDN
                     TGEYILRHEYDDYSRRLNKKLGNGSPYALLQSSSFDTNLRLQTLTNNLSASGATAYDN
                     AYTFGYNSADQLASSGGSTSSYDYAAPSNSISYGIDGLNRVTSVNSSPSFGYDGRGNL
                     TSDGGSGTYSYNANNLLTSAVQSGVTSTLTYDAENRLASITKNGVTTKFLYDGADMIA
                     EYNGSSLLRRYVHGFDVDEPVVSYDGTSTVDSNRHYLVADVRGSIVAVTNSAGDVGAS
                     GTGVNTYDAYGFPGSGNTGRFQYTGQMWLPEIGMYYYKARLYNPVIGRFMQTDPIGYG
                     DGMNWYAYVHGDPINAVDPLGRDCVSRNEDGATSCSYTDEGGFFGDSASYGSNADLAL
                     IGAWQTQVEARFAQMSSPSYDGGYSITKAAFTFGDTTYVFDDEIGKDAHKTLQAAALL
                     LGGPGWFVEASSVQDKRALFGLASFGGGRVDMVYKPWHSLYEIKPEGQQ"
BASE COUNT          487 a          542 c          563 g          429 t
ORIGIN      
        1 tgatcggatc accaaatacg gctatgacgc gctcggcgac ctgacaagca cgaccagcgc
       61 ctacggcacg agcctggcgc ggatcgacgc cgcgaaagtc tacgatatcg gctcatcgac
      121 aagcaccgga accctgcagt acgccacgga cgccaaggga aacaagacga gctacgctta
      181 tgacgggttc aaccgcctga tcaaaacctg ttacccgacg cccagcaatg gcggaaccac
      241 taacaccagc gattgcgatc agacggccta tcggtcggtg cttgtagaca acacgacgac
      301 gcaggcgacg agcctggtcg atcatttggt cttacgcgat ggccagacga tctcatttgt
      361 gtacgatttt ctgggtcggg tcagcgccaa gtcgggtgct gtttcggaga gctacgccta
      421 cgacaattac aacgacgtca ccacccatgt gaacaacacg acgggtggtg cgtccgccag
      481 cgagacctat ggttataatg ccttgggctg gctcttgagt gatacgcaac ccatcggcgc
      541 tgtcagctac ggctatgacg cctacgggcg ccgtaataag ctgacctatc ccgacggttt
      601 ctacgtcacc tatcaatatt ggaatgacga cagcggttgg tacattcaag ataacaccgg
      661 cgaatatatt ctccgtcacg agtacgacga ttacagtaga cgccttaaca agaagctggg
      721 caacgggagc ccttacgccc tccttcaatc atcaagcttc gatacgaacc tgcgcctgca
      781 aaccctcacg aacaatctga gtgcgagcgg ggcgacggcc tatgacaatg cctatacgtt
      841 tggatacaac tccgcagatc aactggcctc gagcggcggt tccacctcca gttacgatta
      901 tgcggcgccg tccaactcca tcagctacgg gattgacggg ttgaatcgag tcacatcagt
      961 gaacagcagt ccttcttttg gttatgacgg tcgggggaac ctgacaagcg acggcgggag
     1021 cggaacctat agctataacg ccaacaatct gttgacatcg gccgtgcagt cgggcgtgac
     1081 gagcacccta acctatgacg ccgagaaccg cctggcgagc atcacgaaaa acggggtaac
     1141 gacaaaattc ctgtacgatg gcgccgacat gatcgccgag tacaacggct cgagcctctt
     1201 gcgccgctac gtgcatggct ttgacgtcga tgagccagtt gtcagttatg acggtacaag
     1261 cacggtggat tccaatcgtc attaccttgt cgccgacgtg cgcgggtcga tcgtggccgt
     1321 gacgaattcc gcgggggatg tcggtgcgtc gggaacgggc gtcaatacct atgacgcgta
     1381 cgggtttccg ggttcaggca atacaggtag gttccagtac actggccaga tgtggctgcc
     1441 tgagatcggc atgtattatt acaaagcccg actctacaac cctgtcatcg gcaggttcat
     1501 gcagaccgac ccaatcggtt atggcgacgg catgaactgg tatgcgtatg tgcatggaga
     1561 tccaattaat gctgtagatc cacttgggag agattgtgtt agtcgtaacg aagatggagc
     1621 aacctcctgt tcctacaccg atgaaggtgg ctttttcggt gattcagcat catatggttc
     1681 gaacgccgac cttgcgttaa taggcgcgtg gcaaacccaa gtagaggcaa ggtttgccca
     1741 aatgtcgtct ccatcgtacg atggaggata ttccataacc aaggcagcgt tcacttttgg
     1801 cgacacgacg tatgttttcg acgacgaaat aggaaaggat gcccacaaaa cgcttcaggc
     1861 ggcggctctc ctcctagggg gccctggttg gtttgtagag gctagttctg tacaagataa
     1921 acgcgcgtta ttcgggcttg cctcctttgg agggggtaga gtggatatgg tttataagcc
     1981 atggcattcg ctgtacgaaa taaagcctga agggcagcaa t
//
LOCUS       DATMTI010000722         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_834607, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000722 DATMTI010000000
VERSION     DATMTI010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834607"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VN039_11565"
     CDS             <1..217
                     /locus_tag="VN039_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46638.1"
                     /translation="CLVEKEVWKEIFMPREVTHGPVRTNIQDRYEVEVDDPAAEQTIE
                     NAIPRGNAALGAAIHECRAHILFFKRS"
     gene            complement(218..1105)
                     /gene="htpX"
                     /locus_tag="VN039_11570"
     CDS             complement(218..1105)
                     /gene="htpX"
                     /locus_tag="VN039_11570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HWV46639.1"
                     /translation="MKWMKGIGLLLIANILIMVTLSLTVPILINVILPMFGIDVRGSV
                     DLSSLVWAAMFGFGGAFISLAFSKQMARAMLNCQQIIQPRSRAEQVVYGSVQEIAQRL
                     HITMPEVWVYDSPDPNAFATGPSKNNSMVAVSTGLLQNLREDEVKAVLAHEMGHVYNG
                     DMFATTVLAGLMNTFVYYIAMWVQRFFAERDQAALGFGLSLVLQIVVSILASIVISWF
                     SRHREFGADAFAARVYGKDSMISALRAIDRWVNRAQFEYSTQDALATMKISGKTGGVM
                     SLFSTHPSIDARIAALEAL"
     gene            1359..1763
                     /locus_tag="VN039_11575"
     CDS             1359..1763
                     /locus_tag="VN039_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWV46640.1"
                     /translation="MVSWLTVTLAIGHAADTLTFSSGSAIAVPVSPDGVQRATVILDS
                     YSYSPNHLVVENGKPVELNLTSITMITPHNFIIKDSAASLSVEQDVGAGRTVVARFVP
                     TQPGLFPFFCDKRLWPMPSHRDKGMEGILEVK"
     gene            1772..>2527
                     /locus_tag="VN039_11580"
     CDS             1772..>2527
                     /locus_tag="VN039_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HWV46641.1"
                     /translation="MSAASSKEELLRDLLKRVSRLFYATLVVVPSNVRDQVSLAYLFA
                     RAADTIADAELIERSRRLGFLNRLREQFLCDQADWAQIHAIQQAMGPLQQDSAERILL
                     ERLDECFRLFLDFSSDDRRRIQRVMTTLTQGMEMDLGTFPGNSVADLTALKTLEDLDC
                     YTYHVAGCVGEFWTSLMCAHRKALADWDIQRMSDVGVRFGKGLQLTNIIKDLAHDLQK
                     GRCYIPEIMLAEAGLKPRDLLDQNNLPRFRPVLS"
BASE COUNT          602 a          649 c          684 g          592 t
ORIGIN      
        1 ctgtctggtc gaaaaagaag tctggaagga aatcttcatg cctcgcgagg taacgcatgg
       61 gccggttcgg acgaatatcc aggatcgata tgaggttgag gttgacgatc ctgccgctga
      121 acagacgatt gagaacgcga tccctcgagg gaatgccgca ttgggcgcgg cgattcacga
      181 gtgccgcgcc cacattttgt tctttaagag atcgtgatca gagcgcttcc aatgccgcga
      241 tccgagcgtc aatcgatggg tgagtcgaga acaagctcat gactccaccg gtttttcctg
      301 aaatcttcat ggttgccagt gcgtcctgtg tcgagtattc gaactgagca cgattgaccc
      361 agcgatcaat cgcccttagc gcgctgatca tggagtcctt cccatagacc ctggccgcaa
      421 atgcgtcggc tccgaactcg cgatggcgtg agaaccagct gatgacgata gaggccaaaa
      481 tactgacgac gatctgaagg accagtgata ggccgaatcc caatgccgct tgatctcgtt
      541 ccgcgaagaa acgttgcacc cacatggcga tgtaatacac aaaggtattc atcagcccag
      601 ccaggacggt cgtggcgaac atgtcgccgt tatacacatg acccatttcg tgagccagaa
      661 ccgccttgac ttcgtcttct ctcaagttct gaagcaaacc ggttgaaacg gccaccatgg
      721 aattgttttt gctgggtccg gtcgcgaacg cattggggtc cggtgaatca tagacccaca
      781 cttcgggcat cgtgatgtgc aggcgttgag cgatttcctg tacactgccg taaacgacct
      841 gttcagcgcg tgagcggggt tgaatgatct gctggcaatt gagcatcgcc cgcgccattt
      901 gtttggagaa tgccaaactg atgaatgcgc ccccgaaccc aaacattgcc gcccatacca
      961 atgacgagag atcaacagag ccacggacat cgattccgaa catcggcagg atgacattga
     1021 tcagaatcgg cacggtgagc gagagcgtca ccatgatgag aatgttagcg atcagcagta
     1081 ggcctatgcc tttcatccac ttcatcgaga gcctccttga agacaagcac tcgcctcaca
     1141 cgatgtgagt aacgagtccc actggattat acacaagttc ttcccctgca atacactggg
     1201 gagagcaggt gattcattaa actgtgaata agacttggcg attaaaagtc aagacctcag
     1261 aagtctctga aagtacgtgg ataatgttga ccatctcccg tcgttactgt tagaaagttc
     1321 tcatgggagg catcgggaaa tcttgttgtg cgcttggcat ggtgagctgg ctgactgtca
     1381 cgctcgcaat aggccatgcc gcagacacac ttacattttc atcaggttcg gcaatcgctg
     1441 tccctgtctc tccggatgga gttcaacggg ccacggtcat tctcgacagc tattcctatt
     1501 cgccgaacca tctggttgtg gaaaacggga agccggttga gctgaatttg accagcatca
     1561 caatgattac gccgcataat tttatcatca aagactcggc cgccagcttg tcggtcgaac
     1621 aggatgtcgg agctggaagg accgtcgtgg ccagattcgt cccgactcag cctggcctct
     1681 ttccgttctt ttgcgacaaa cgattgtggc ccatgcctag ccatagagac aaaggaatgg
     1741 aaggaatact tgaagtgaaa tgatgcatgc catgagtgcc gcgtcttcga aagaggaact
     1801 gcttcgtgat ctgctcaagc gtgtgtctcg cctcttctat gcgacgctgg tggtcgtacc
     1861 gtcgaacgtc cgcgatcaag taagtttggc gtatctcttt gccagggctg ccgatacgat
     1921 cgccgatgcg gagctgatcg agcgatcgcg tcgtctcggt tttctcaacc gcctcagaga
     1981 gcagtttctc tgtgaccagg cagactgggc acagatccat gcgattcaac aggctatggg
     2041 gccgcttcaa caagattcgg ctgagcgaat cctgcttgaa cggctggacg agtgttttcg
     2101 gctctttctc gacttttcat cggacgaccg acgtcggatt cagcgggtaa tgacgaccct
     2161 gacgcaagga atggaaatgg atctgggaac gttccctgga aattccgtgg cggatctcac
     2221 cgcgctcaag acgctcgagg acttagattg ttacacctat catgtggctg gatgtgtggg
     2281 tgagttctgg acttccttga tgtgtgctca tcgcaaagca ctggcggact gggacattca
     2341 acggatgtcg gatgttgggg tacgatttgg aaaggggctg caactgacaa acatcatcaa
     2401 agatcttgct catgatttgc agaaaggacg gtgttacatt cccgaaataa tgctcgccga
     2461 ggcagggctc aaacctcgag atttattgga tcagaacaac ctccctcgct tcagacctgt
     2521 gctgagc
//
LOCUS       DATMTI010000723         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_27362, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000723 DATMTI010000000
VERSION     DATMTI010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27362"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN039_11585"
     CDS             <1..322
                     /locus_tag="VN039_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46642.1"
                     /translation="PWLTRLRGLIQKSLTQRRNIVLACSALKRAYRRYLRVSPEVAFV
                     YLRTETHVLEERLKRRRGHFADPVLLQSQLNTLEVPLDDTLTVDTTRTPAEIVEIIRK
                     ALHI"
     gene            432..1697
                     /gene="glgC"
                     /locus_tag="VN039_11590"
     CDS             432..1697
                     /gene="glgC"
                     /locus_tag="VN039_11590"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWV46643.1"
                     /translation="MSGTYIAIMGRVRVLGMIMAGGKGERLMPLTAVRSKPAVPFGGK
                     YRIVDFVLSNMLNSGITANYVLVQYRSQSLIEHVRESWSIAGRLKDQFITIVPPQMRA
                     GGGWYEGTADAVYHNLNLIADFRPNVVAVLGADHIYRMDVSQMIQFHQDSGAEVTVAA
                     LPVPSEQSSQFGILEVDADDRLVGFEEKPTKAKTMSGCPTMALSSMGNYIFDASVLLR
                     TLERDAASPGSHDFGRNIIPDLIGGRRKVFAYDFLRNDVPGLRSYEERGYWRDVGTLD
                     AYWQANMDLLGETPSFDLRNNQWPVVSAGYESPMASIVRTYTDQSMIGQGSHIVDAEI
                     RNSVIGRDVHIEAGARLEGCIIMDGVRIGAQARLHRVIADRFSSIPAGSDIGFQESPQ
                     LPGCHLSPTGLVIIPRDTKVAMTLMQSVS"
     gene            1712..>3240
                     /gene="treY"
                     /locus_tag="VN039_11595"
     CDS             1712..>3240
                     /gene="treY"
                     /locus_tag="VN039_11595"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HWV46644.1"
                     /translation="MPGRRVLATYRLQLNIDFGFRHARDVTAYLHDLGITHCYSSSIL
                     AAMPGSMHGYDVIDPSRINPELGSEEEFKAWYRSLHDHDMGLILDIVPNHMGIAKALN
                     RWWYDVLENGPSSRYAGAFDIDWHPLKRELHNKVLLPILSDHYGIVLENQEMSIAYED
                     DRFVVRCGDCRLPLAPQSWSTVLSYRLDALLCDDTAQAESEGLTELQSILTAIRHLPN
                     GEDSTPEGREEREREKEVIRKRIAVLMANNQRITAFVQDNMRLFNGTAGEPCSFDLLD
                     ELLNQQAYRLASWKVASEEINYRRFFDINELAAIRVEESSVFEATHEVVFKLLRDGVA
                     SGVRIDHVDGLYDPEGYLQRLQSWARRELSGDSSTERPLFVVVEKILGLDEPLPESWP
                     IEGTTGYAFLNTVNGLFVDHTRERAFSDLYRRITNRSQPYADMVYEAKQLIMRASMAS
                     EINVLGHQLNLLSERDRRSRDFTLNSLTNAIREIIACFPVYRTYVTVGAEPVSERDNA
                     S"
BASE COUNT          726 a          878 c          936 g          700 t
ORIGIN      
        1 gccatggctc acccgcctcc gagggcttat tcaaaagagc ctgacccaac gtcggaatat
       61 cgtactggcc tgttcagccc tcaaacgtgc gtaccgccgg tatttacgcg tcagtcccga
      121 agtggcgttc gtgtatttac ggactgaaac tcatgtgctc gaagagcgtc tcaaacgacg
      181 ccggggccat ttcgccgatc cggtcttgct ccaaagtcaa ttaaatacat tggaagtacc
      241 tctcgacgac actctgaccg tcgatacgac gcgcactcct gccgaaattg tcgagattat
      301 tcgtaaggcg ttacacatct aataagttaa tcaaattaat aggatggtga aaagtccgcc
      361 agcggcgttc tcgcgggaca ctgtcgcctc accattctcg gcggcgaggg gtttcccgag
      421 tatcgaaagg gttgtctgga acatacattg ccatcatggg acgtgtgcgg gttctgggca
      481 tgattatggc gggaggaaaa ggcgagcgcc tcatgccgct cacagcggtg cgcagcaagc
      541 cggccgtgcc gttcggcggc aagtatcgca tcgtcgattt cgtcctcagc aacatgctca
      601 actccggcat caccgccaac tatgtgttgg tgcaataccg atcgcaatcg ttgatcgaac
      661 atgtgcggga atcgtggagc attgcaggcc ggctcaaaga ccagttcatt acgatcgtgc
      721 cccctcagat gcgggcgggc gggggatggt acgaagggac cgctgatgcg gtctatcaca
      781 atctcaattt gattgcggac tttcgcccca atgttgtggc ggtcttggga gccgaccata
      841 tctatcgaat ggatgtttcg caaatgatcc aatttcatca ggacagcgga gctgaagtga
      901 cggtggcggc attgcccgtg ccgagcgagc aatcgtccca gttcggtatt ctcgaagtcg
      961 atgccgatga tcgactggtc ggattcgaag aaaagcccac aaaggccaag accatgtcgg
     1021 gatgtcccac gatggctctg tcctccatgg ggaactatat cttcgacgcc tccgtgctgt
     1081 tgcgcacatt ggaacgggat gccgccagtc cgggcagcca cgatttcggc cgcaatatca
     1141 ttccggacct catcggcggg agaagaaagg tattcgcgta tgattttctg cggaacgatg
     1201 tgccgggcct gcggtcgtac gaagaacgag gatactggag ggatgtcggt acactggatg
     1261 cctactggca ggcgaacatg gatctgttgg gcgagacgcc gtcgttcgat ctccgaaaca
     1321 atcagtggcc ggtcgtctcg gccggatacg agagccccat ggcgtccatc gtgcgaacct
     1381 acacggatca atccatgatc ggtcaaggca gtcatatcgt ggatgcggaa atcagaaatt
     1441 ccgtaatcgg gcgcgacgtc cacatcgagg ccggcgcgcg tcttgagggg tgcatcatca
     1501 tggacggtgt gcgcatcggg gctcaggctc ggctgcatcg cgtgatcgcc gaccgcttca
     1561 gcagtattcc cgccgggagc gacatcggtt tccaggaatc gcctcaactt cccggatgcc
     1621 atctctcgcc gacaggcctg gtcatcattc cgcgcgacac caaagtggcg atgactctga
     1681 tgcaatccgt ctcctgagct atggagtcgt gatgcccgga cgtcgtgtgc tggccacata
     1741 tcggcttcaa ctcaacatcg atttcggatt ccgccatgcg cgtgacgtga ccgcctatct
     1801 gcacgacctc ggaatcacgc attgttacag ctcctccatt ctggccgcga tgccgggcag
     1861 catgcatggc tacgatgtca tcgatccctc gcgtataaac ccggaactcg gatcagagga
     1921 ggaattcaag gcgtggtacc gcagtttgca tgaccacgac atggggctga tcctggatat
     1981 cgttcccaat cacatgggga ttgccaaagc gctgaatcgg tggtggtacg acgtgctgga
     2041 aaacggaccg tcatcccgtt atgcaggagc attcgatatc gactggcatc ctctgaagcg
     2101 ggagttgcac aacaaggtct tgctcccgat cttatcggat cactacggca tcgtgctgga
     2161 aaatcaggaa atgagcatcg cctacgaaga cgaccgattt gtggtccgct gcggagactg
     2221 ccggcttccg ctcgctccgc aatcgtggag caccgtctta tcgtaccgac ttgatgcatt
     2281 gttgtgcgac gacacggcgc aggccgaaag tgaaggactc acggaactgc aaagcatttt
     2341 gacggccata cgccatcttc ccaatggcga ggatagcact ccggaggggc gagaggagcg
     2401 cgaaagggag aaagaggtca tccgcaaacg tatcgccgtg ctcatggcga acaatcaacg
     2461 aatcacggca ttcgttcaag acaatatgcg cctctttaac ggtaccgccg gagaaccatg
     2521 cagtttcgat ctgctggacg agttgctcaa tcaacaggcc taccggttgg catcgtggaa
     2581 ggttgcctcg gaggagatca actaccgacg attttttgat attaatgaac ttgccgcgat
     2641 tcgtgtggaa gagagttcgg tgttcgaggc gacccatgag gtagttttta aactccttcg
     2701 agacggtgtg gcgtcgggag tgagaatcga ccatgtcgat gggctgtacg atcccgaggg
     2761 gtatcttcag cgactgcaga gttgggcaag gcgcgagttg agcggcgact cgtcgacaga
     2821 gcggccgctg tttgtcgtgg tggaaaaaat cctcggactc gatgaaccgc tgcccgagtc
     2881 ctggcctatc gaggggacca cgggatatgc gtttctcaat acggtaaacg gattattcgt
     2941 agaccatacg cgggagcgag ccttcagtga tctctatcgg cgcataacca accgttccca
     3001 gccctatgcc gatatggtct acgaagcaaa acaacttatc atgcgggcct ccatggcgag
     3061 cgagatcaac gtgctcggcc atcagctcaa tcttctgtcc gagagagatc gacgatcgag
     3121 agacttcacg ctgaatagtc tgaccaatgc cattcgcgaa atcatcgctt gtttccctgt
     3181 atatcggaca tacgtgacgg tgggggcgga acccgtcagc gagcgggaca acgcgtccat
//
LOCUS       DATMTI010000724         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_451607, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000724 DATMTI010000000
VERSION     DATMTI010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451607"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VN039_11600"
     CDS             <1..394
                     /locus_tag="VN039_11600"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV46645.1"
                     /translation="VMDGYNQHRHEGEEMARFPHTRSYKRFLFHSPLIVGGESCVCEG
                     TLRDLSMHGCSMVCDREFILGSKVRASLLLPDQTSALSIELGRITWVKGRECGVEFMQ
                     LPLQSRLRLNRTLRIPLIQSLNAWKNRE"
     gene            complement(506..1234)
                     /locus_tag="VN039_11605"
     CDS             complement(506..1234)
                     /locus_tag="VN039_11605"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HWV46646.1"
                     /translation="MKEKKGKGEDKDTDKVVEIGGRSDPFQRLLGLMFKAHPWHGVSI
                     GDEAPDVVTAYIEIVPTDTVKYEVDKSSGFLKVDRPQRFSNFCPVYYGLIPQTYSGER
                     VANIFAKRARRKDMVGDGDPLDICVLSEKSIPHSDILLTALPIGGLSLADGGEADDKI
                     IAVMRDDAVYGNLTDISQCPTRLLERLQHYFLTYKSAPGTTHHKVEITGIYGREESLK
                     VIRASHADYRVKYPELSSLWPKNV"
     gene            complement(1453..>2887)
                     /locus_tag="VN039_11610"
     CDS             complement(1453..>2887)
                     /locus_tag="VN039_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46647.1"
                     /translation="YGFNHAEAIRSFTRAAELDPTCAMCYWGIALAHGPHVNAPMDST
                     SGIAAYAAVQKALALKSHASTPERAYIEALAQRYEADPPADRARLDMLYSRAMGQVAK
                     TYPKDLDAATLYAESLMDLRPWNYWRPDGTPYPETKEIVRRLEKVLTRNPNHPGACHY
                     YIHAVEAVNPKAAVPCAERLARLMPGEGHMVHMPSHIFIRVGRWNDAVQSNHHAIHTD
                     EEFIEGQRPMGVYPLAYYPHNIHFFAFASTMAGRSAQAIEASNTLTTKVNLDAARQVG
                     LLQEMLPYHALTLTTFGRWDEVLGQSLPPEDIRFSYAMASYARGVAHAAKGEWGEAQS
                     ALDTVTSAEAATPEGAEGKAALSIAVHALSGEIATRRGDLDAGITHFRNAAKIEDAGL
                     YFEPPKWYYPIRHSLGAALVKAGQNAEAEKVYREDLHRFPENGWSLFGLAQALRAQGK
                     NTEADATEARFRRAWSDTDVTLTASRF"
BASE COUNT          550 a          849 c          849 g          639 t
ORIGIN      
        1 ggtgatggac ggatacaatc agcatcgtca cgaaggagaa gagatggccc gctttcctca
       61 tacgcgaagc tacaaacggt ttctcttcca ttctccgctg attgttggcg gcgagtcgtg
      121 tgtctgcgag ggtacgctca gggatctctc gatgcacggg tgttcgatgg tatgcgatcg
      181 tgaattcatc cttggcagca aggtgcgcgc gagtctgctc ttgccggatc agacatcggc
      241 cttatctatt gagctgggcc ggataacctg ggtgaagggc cgcgagtgtg gagtcgagtt
      301 tatgcagctg ccgttgcaat cgaggttgcg cctcaatcgc acgttgcgaa tcccactcat
      361 ccaatccctg aacgcgtgga agaatcggga atgacagaaa cctgccgttt aggatttgta
      421 gcctctggtc ccgcctgata agaatcttgt acagggcgtt ccactcgcaa ctacatgaag
      481 agatcaccaa tcccacacat tcttcctaca cattcttcgg ccacaaggaa ctcagctccg
      541 gatacttcac ccgatagtca gcgtggctag cgcgaatgac tttcagcgat tcctcccgtc
      601 cgtatatgcc ggtgatttca accttgtgat gagtcgttcc aggcgcgctt ttatacgtca
      661 aaaaataatg ttgcagacgc tcaaggagcc tcgtcggaca ttgcgagatg tccgtgagat
      721 tgccgtacac ggcatcgtcc cgcatcacag caatgatctt gtcgtcggcc tcgccgccgt
      781 cggccaggct caatccaccg atcggcagtg ccgtcaggag aatgtcgctg tgcggaatgc
      841 tcttttccga cagcacgcag atatcgagcg gatcgccatc cccgaccatg tccttgcgac
      901 gggctcgctt ggcgaagata ttggccaccc gctctccgga gtaggtctgc ggaatgaggc
      961 catagtacac cggacaaaaa ttggaaaacc gctgaggcct atccactttg agaaagccgc
     1021 tgctcttgtc cacttcgtat ttgaccgtgt cggtcgggac gatttcaatg taggccgtaa
     1081 ccacgtccgg ggcttcatcc ccaatagaca caccgtgcca cggatgtgcc ttgaacatca
     1141 gcccgagcag gcgttgaaag ggatcgcttc ttccacctat ctcaaccacc ttgtctgtgt
     1201 ccttatcctc tccctttccc ttcttctctt tcatgtctcc tccccattta ccgaccaccc
     1261 cagcgacacg gactctttca atcggtcagt atcacctagg agctcgatag gagcaatagt
     1321 ttctcacggc ctaaatgatg tgatttggag gactgccgtt tttatgctca caggagcgta
     1381 ccaagagggt cggaaggcct gcgccattcc gacccaaaac tcccccgacc tctttttgtc
     1441 gatcgctatc cgctagaatc tcgatgccgt gagcgtcaca tcggtgtctg accacgctcg
     1501 gcggaagcgt gcttcggttg cgtccgcttc agtgttcttc ccctgcgccc ggagcgcctg
     1561 tgcgaggccg aacagagacc aaccgttctc tgggaagcga tgaaggtctt cgcgatacac
     1621 cttctcggcc tcggcatttt gtcccgcttt caccagcgcg gcgccgagtg agtgccgaat
     1681 cgggtaatac catttgggcg gttcgaagta gagtcccgcg tcctcgatct tggcggcatt
     1741 ccggaagtgg gtgatacccg cgtcgaggtc gccgcggcga gtcgcgattt cgccggacag
     1801 cgcgtgtacg gcgatcgaga gagccgcctt cccctcggca ccttcgggcg ttgcagcctc
     1861 ggccgacgtc actgtgtcga gcgccgattg cgcctccccc cactctcctt tggcggcgtg
     1921 ggccacgccg cgggcatagg aggccatggc gtacgaaaaa cggatgtctt caggtggaag
     1981 agattggccg agcacctcgt cccatcttcc gaatgtcgtg agcgtgagcg cgtggtacgg
     2041 cagcatttct tgaagaagcc caacctggcg cgcggcatcg aggttcacct tggtggtgag
     2101 tgtattggat gcttcgatcg cctgcgcact acggcccgcc atcgtggaag cgaaggcgaa
     2161 aaagtggatg ttgtgcggat agtacgcgag cggatagaca cccatcgggc gctgcccttc
     2221 gatgaactcc tcgtccgtat ggatcgcatg gtgattggac tgcaccgcgt cgttccagcg
     2281 gccgacgcgg atgaagatgt gcgagggcat gtgcaccata tggccttccc ccggcatgag
     2341 ccgggcgagc cgttcggcgc acggcaccgc cgccttcgga ttcaccgctt cgaccgcgtg
     2401 gatgtagtag tggcacgcgc cgggatgatt ggggttacgc gtgaggacct tctccagccg
     2461 gcgtacgatt tccttggttt cggggtaggg agtgccgtca ggacgccagt agttccatgg
     2521 gcggaggtcc atcagcgatt cagcgtacag ggtcgcggca tcgaggtcct tggggtaggt
     2581 cttggcaacc tgccccatcg cacgcgaata caacatgtcc aaccgggcac gatcggccgg
     2641 gggatccgcc tcgtagcgct gcgcgagcgc ctcgatgtac gcgcgctcag gcgtcgaggc
     2701 gtgggatttg agtgcgagtg ctttctgcac cgccgcatag gcagcaattc cgctcgtcga
     2761 gtccatagga gcgttgacgt gcggcccgtg ggcaagtgcg atcccccagt agcacatggc
     2821 acaggtggga tcgagctctg cggcgcgcgt gaacgagcgg atcgcttccg catggttaaa
     2881 gccgtag
//
LOCUS       DATMTI010000725         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_409833, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000725 DATMTI010000000
VERSION     DATMTI010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409833"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VN039_11615"
     CDS             <1..1344
                     /locus_tag="VN039_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate oxidoreductase subunit alpha"
                     /protein_id="HWV46648.1"
                     /translation="NSWMEFTYPEMTATVSNPWIQLWKGGIRPLYDTRNDADTFAGVA
                     AKLTEMTGDTRMRDVFHFVYQNRVDVYAQRLLDASSTFYGYSADVLLKSEKGWMVMTR
                     TYPRHPLWEETNESKPMWTRSGRIESYRIEPEAIEYGENFIVHREGPEATPYLPNAIF
                     TTNPYVRPDDYGIPITAQHHDDKTIRNIKLSWVEIKRHSNPLWEKGYQFYCVTPKTRH
                     RVHSQWSVNDWVQIYESNFGDPYRMDKRTPGVGEHQVHINPQAAKDRGINDGDYVYID
                     GNPVDRPYRGWKPSDPYYKVARLMIRAKYNPSYPYHVTMAKHAPFVSTPKSVKGHETR
                     PDGRAIAIDTGYQSNFRYGAQQSFTRSWLMPMHQTDSLPGKSANGLKFKWGFEIDHHA
                     VNTVPKECLIRITKAEDGGIGARGPWEPVRTGFTPGQENEFMIKWLKGEHIKIKV"
     gene            1444..2733
                     /locus_tag="VN039_11620"
     CDS             1444..2733
                     /locus_tag="VN039_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWV46649.1"
                     /translation="MPEVYNWQLGRKMLYPYEERHPKWQFAFVFNINRCLACQTCSMA
                     DKSTWLFSKGQEYMWWNNVETKPYGGYPQFYDVKITQLIEQVNPGGQVWNVRVGRKHH
                     APYGVFEGMTIFDAGAKVGQAAIGYIPTDQEWRFVNIYEDTATSMRALVEGIDKTGFS
                     RDEPWKMTGSSLPEHETFYFYLQRICNHCTYPGCLAACPRKAIYKRPEDGIVLIDQNR
                     CRGYKKCVEQCPYKKPMYRGTTRVSEKCIACYPRIEGKDPLTGGEPMETRCMAACVGK
                     IRMQSLVRIGEDGLWAEDRWHPLYYAIRVEQVALPLYPQWGTEPNGFYIPPRHSPRGY
                     ARQMFGPGVDNAIEKYLVPSRELLAVLQLWRASQQIVFRYDVIPGPKVFETQIHGKRF
                     EMYNDTVLGFNKSGKEVARIQVEEPIYIRPAERVTWL"
BASE COUNT          625 a          790 c          845 g          508 t
ORIGIN      
        1 aactcctgga tggagttcac ctatccggag atgacggcca cggtgtccaa tccgtggatc
       61 cagctctgga agggcggcat ccggcccttg tacgacacgc ggaacgatgc ggatacgttt
      121 gcgggcgtgg cggcgaaact gaccgagatg acgggggaca cccgcatgcg cgacgtgttc
      181 catttcgtct atcagaaccg cgtggatgtg tatgcccaac gactcctgga cgcctccagc
      241 actttttacg ggtacagtgc cgatgtgctg ctgaagtccg aaaagggctg gatggtcatg
      301 acccgcacct acccgcggca tcccctctgg gaagagacca acgaatcgaa accgatgtgg
      361 acgcggtccg ggcgcattga gtcctatcgc atcgaaccgg aggccatcga gtacggcgaa
      421 aacttcatcg tgcaccggga agggccggag gcgaccccct acttgccgaa cgccatcttc
      481 acgacgaacc cctatgtccg gccggacgac tacggcattc cgatcacggc gcagcaccat
      541 gacgacaaga cgattcggaa tatcaagctc tcgtgggtcg aaatcaagcg ccacagcaat
      601 cccttgtggg aaaagggcta ccagttctac tgcgtcacgc ccaaaacgcg gcaccgggtt
      661 cacagtcagt ggtcggtgaa cgactgggtg cagatctacg agtcgaactt cggggatccc
      721 tatcggatgg acaagcggac cccgggcgtc ggcgagcatc aagtccatat caatccgcaa
      781 gcggccaaag accggggaat caacgacggg gactatgtgt acattgatgg gaacccggtg
      841 gaccgcccct accgcggctg gaagcccagt gatccctact acaaggtggc ccggttgatg
      901 attcgcgcga agtacaatcc gtcctatccg taccatgtca cgatggccaa gcatgcgccc
      961 ttcgtctcga cacccaagtc ggtcaagggc catgaaacga ggccggacgg gcgcgccatc
     1021 gcgatcgaca cgggctacca gtccaacttc cggtacgggg cacaacagtc ctttacgcgc
     1081 agttggctca tgccaatgca tcaaaccgac tcgctgccgg ggaaaagtgc gaacgggctc
     1141 aagtttaagt gggggttcga aatcgaccac catgcggtca ataccgtgcc gaaggaatgt
     1201 ctcatccgca tcaccaaggc ggaagacggc ggcatcggcg cgcggggacc gtgggaaccg
     1261 gtgcggaccg gctttacgcc cggccaagag aacgagttca tgatcaagtg gctcaaaggc
     1321 gaacatatta agatcaaagt ctaaccactc gtgaggggtg aggcgtcagg ggtgaggggg
     1381 gaatgaagcc gatttcacct ccctcgcctc ccgccttacg cttcacgcct tacggaggaa
     1441 accatgcctg aagtctataa ttggcagctg ggccgcaaga tgttgtatcc ctatgaggag
     1501 cggcatccga agtggcagtt tgcctttgtg ttcaacatca atcgctgttt ggcctgtcag
     1561 acctgtagca tggctgataa gtccacctgg ctcttctcca aagggcagga atacatgtgg
     1621 tggaacaacg tggaaaccaa gccgtacggc gggtatccgc agttctacga tgtcaaaatc
     1681 acgcagctga tcgagcaggt caatccgggc ggccaagtct ggaacgtccg cgtcgggcgg
     1741 aaacaccatg ccccctacgg ggtgttcgaa ggcatgacga ttttcgatgc gggggccaaa
     1801 gtgggccagg ccgcgatcgg ctatattccg acggatcagg aatggcggtt cgtcaacatc
     1861 tacgaagaca cggccacctc gatgcgcgcc ctcgtcgagg gcatcgacaa gaccggcttc
     1921 tcgcgcgatg aaccgtggaa aatgaccggc agcagtctcc cggaacatga gaccttctac
     1981 ttctatctcc aacggatttg caatcactgc acctatccgg gctgtttggc ggcctgcccg
     2041 cgcaaagcca tttacaagcg gccagaagac ggcatcgtgc tgatcgatca gaaccgctgc
     2101 cgggggtata agaaatgtgt cgagcaatgt ccgtataaaa aacccatgta ccgggggacg
     2161 acgcgggtca gcgagaagtg tatcgcctgt tatccccgga tcgaaggcaa agatcccttg
     2221 accggcggcg aaccgatgga aacgcgctgc atggcggcct gcgtggggaa gattcgcatg
     2281 cagagcctgg tccggatcgg tgaagacggg ctgtgggccg aagatcggtg gcacccgctg
     2341 tactatgcga tccgggtgga gcaggtggcc ttgccgttgt atccgcaatg ggggaccgag
     2401 cccaatggct tctatattcc cccccggcat tccccccggg gctatgcccg gcagatgttc
     2461 gggcccggcg tggacaacgc cattgaaaag tatctggtgc ccagtcggga attgttggcg
     2521 gtgctgcagc tctggcgcgc cagccagcag atcgtgttcc gctacgatgt gattccgggg
     2581 ccgaaggtgt ttgaaaccca gatccacggg aagcgcttcg agatgtacaa cgataccgtg
     2641 ttgggcttca acaagtcggg caaagaagtg gcgcgcattc aggtcgaaga gccgatctac
     2701 attcggccgg ctgaacgcgt gacctggctg taacagacag gccgcgatga ttcacgaggc
     2761 aggagtct
//
LOCUS       DATMTI010000726         1698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_451648, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000726 DATMTI010000000
VERSION     DATMTI010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451648"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /gene="ccsB"
                     /locus_tag="VN039_11625"
     CDS             <1..495
                     /gene="ccsB"
                     /locus_tag="VN039_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HWV46650.1"
                     /translation="VPLQPALQSYWLVIHIIVAVLGTAFFALGFALSGLQLLQYRRER
                     QVAESRPQQFAFLTTLPGSVALENLAYRINIVGFIAWTFTLIAGAIWAEKAWGRYWGW
                     DTKEVWTFIIWVIYAGYIHARATRGWRGSRSAWLAIIGFAAVLFNFGIVNVFFHGLHA
                     YSGL"
     gene            1176..>1698
                     /locus_tag="VN039_11630"
     rRNA            1176..>1698
                     /locus_tag="VN039_11630"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          375 a          473 c          514 g          336 t
ORIGIN      
        1 gtcccgcttc agcccgcact ccagtcgtac tggctggtca tccacatcat cgtggccgtg
       61 ctgggcacgg cgttcttcgc actcggcttc gcgttgtcgg gactgcagct gctccaatac
      121 cgccgcgaac gccaggtggc ggagtcgcgt cctcagcagt tcgcgttcct cacgaccttg
      181 ccgggctcgg tcgcgctgga gaacctggcc taccggatca acatcgtcgg attcatcgcc
      241 tggacgttca cgctcatcgc cggcgccatc tgggccgaga aggcgtgggg ccggtattgg
      301 ggttgggaca ccaaagaggt ctggaccttc atcatctggg tgatctatgc cggctacatc
      361 cacgcgcggg ccactcgcgg ctggcgcgga tcccgctccg cgtggcttgc gatcatcggc
      421 ttcgccgccg tgctcttcaa cttcggcatc gtcaacgtct tcttccacgg actccacgca
      481 tactcgggac tttgacgcac ggagacccgc agaaaccacg accccttcga gcagtcgctc
      541 ggaggggttt tctgtcggtc gaggacgact gagcggggaa gatccgcgtg attccgcgga
      601 agtggagcac gacacgcccg ggatgatggc ggaatttgca cgtccttcgg ggcctgcgta
      661 aagtacttac ttgtcacccc aaaggtgcgg gagagcggaa gagctccccg gcctcaagcg
      721 ggaccgaatc ccaaagccaa gcggcaatct gaacttgaat tagttcggtc gctgagctta
      781 ggattaacac ccactcacag agcaggtctg actggttcag ttcgtgagat cggttgtgcc
      841 cttatacggg tcaacgagat cacggcgcgc cgatttgaca aactgactgc gagtggtaaa
      901 gtagagaagt tgcctcgcgg agacagccgg aaggttgaaa agcgagagcg tccgatcctt
      961 gagaactcaa cagcgtgcac aatgtcaaat gccaaataac ctcggcattg actgtctaga
     1021 cctagtctag cggtcgatgc gagattcctt tggattagaa acagaatgtc agtagatatt
     1081 cgaaactagt caagacaaac tcgcagtgca cggacacatt tcctgtccga gcctgcacca
     1141 tgtttcccga tcgcttgcga tcgcggagct aaacatttac ggagagtttg atcctggctc
     1201 aggacgaacg ctggcggcgt gcttaacaca tgcaagtcga acgatgaacc cggagcttgc
     1261 tctgggggat tagtggcgaa cgggtgagta acacgtgagt aacctgccct tgactctggg
     1321 ataacctccg gaaacggaag ctaataccgg atacgacgta cggaggcatc tcctgtacgt
     1381 ggaaagaact tcggtcaagg atggactcgc ggcctatcag gtagttggtg aggtaacggc
     1441 tcaccaagcc tacgacgggt agccggcctg agagggtgac cggccacact gggactgaga
     1501 cacggcccag actcctacgg gaggcagcag tggggaatat tgcacaatgg gcgcaagcct
     1561 gatgcagcaa cgccgcgtga gggatgacgg ccttcgggtt gtaaacctct tttagtaggg
     1621 aagaagcgaa agtgacggta cctgcagaaa aagcaccggc taactacgtg ccagcagccg
     1681 cggtaatacg tagggtgc
//
LOCUS       DATMTI010000727         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_834789, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000727 DATMTI010000000
VERSION     DATMTI010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834789"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1628
                     /locus_tag="VN039_11635"
     CDS             303..1628
                     /locus_tag="VN039_11635"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV46651.1"
                     /translation="MFVGILIISAISYHNTSILLTNGELDSRSHDLIQLLNNIDAAMS
                     EAEDAHRRYLVTGEASYLEIYKKVVKQKPTFIAYLNDLIRESPGQQQRVGLLDRMMDR
                     QLSAESKATVQFQEGGFRAVKKMAFEGAGKRELGVIHQVITEMDLHERQAVGQRVVES
                     AAGTRNTIVLLGIGALLQLVLLASVYYLIRHDITERRRVAGELQRRGELLEAANKELE
                     SFSYSVSHDLRAPLRHIDGYAVLLRNSVEHSLNDKAARYLQTISDAAKQMGQLIDDLL
                     VFSRMGRQEMLRTTVNLDQLINGILSDLRLDLQARAISWTIAALPEVQGDPAMLRQVF
                     MNLIANAVKFTSTRPSAAIEIGVEHPSSTEIVLFVRDNGVGFDMQYAPKLFGVFQRLH
                     RADEFEGTGIGLANVRRIVHRHGGRTWAEGVPDKGATFYVLLPARRLDS"
     gene            1625..2080
                     /locus_tag="VN039_11640"
     CDS             1625..2080
                     /locus_tag="VN039_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46652.1"
                     /translation="MTVAKPILLAEDNPRDAELALAAMEAEHISDKVVLCHDGAEVLD
                     YLYCRGQFKSRLRGNPAVVFLDLKMPKVNGLEVLRTIKADANLRPIPVVMLTSSREER
                     DLADSYALGANAYVVKPVEFHKFLSAVKELGTFWGIINEPPPESCRSTQ"
     gene            2088..>2572
                     /locus_tag="VN039_11645"
     CDS             2088..>2572
                     /locus_tag="VN039_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46653.1"
                     /translation="MTTLLRVLHIEIDPNDTDRIETLLTDGGLSCAIRRVESRHAFTS
                     ALNGGQVDLILAEFSMPEFDGGGALELAQQAAPDVPFIFVSASLTETACIEQMQRGAT
                     DCIPKESLARLVPSVRRVLREQQARAARMQAEKALLQSEMQLRQLQKLEAVGRLAGGL
                     A"
BASE COUNT          568 a          686 c          736 g          582 t
ORIGIN      
        1 ctccgggtcc cccgctgggt ggttagtttt gtgcttccgc ctcccaagag ttctgctaca
       61 attatggcca tagagctacg atcttactgt atggcttccg tacgatcacc gaagtgtctt
      121 gatgcaagtc gtcctgatcg atggaatcag cctccaatca ttcctcgcag gcctcccgcg
      181 tagcggccga gaggcctaaa tcgtcggcct ccggtgcagg tgcatccgtt ttctccctcg
      241 gttcagttgc gtggtttcac aggctgacca ttgagcagcg ggtcttagcc ggtttcagtc
      301 tggtgttcgt tgggattctg atcatcagcg cgatctccta tcacaatacg agcatcctgc
      361 tgaccaatgg tgagttggat agtcgcagcc atgatctcat ccaacttctg aataatatcg
      421 atgccgcgat gagcgaggca gaggatgccc accggcgtta tctggtgacg ggagaagcgt
      481 cgtatctgga gatctacaag aaggtcgtaa agcagaaacc gaccttcatc gcgtatctca
      541 atgacttgat ccgtgaatca ccggggcaac agcaacgagt cggactcctc gatcggatga
      601 tggaccggca actgagcgcc gagtcaaaag ctaccgtcca gtttcaagag ggcggctttc
      661 gagccgttaa gaaaatggct ttcgaaggag ccggaaagcg tgagctcggc gtcattcacc
      721 aagtcattac ggagatggat ttgcatgagc ggcaagcagt cggacaacgt gtcgtggaat
      781 ccgccgccgg gacaaggaat accatcgtac ttctcggtat cggtgcattg ctgcaactgg
      841 ttctgctggc atcggtatac tacctcattc ggcatgacat cacggaacgg cgacgtgtcg
      901 ccggagagct ccaacgtcga ggagaactct tggaggccgc aaacaaggag ctggaatcat
      961 tcagctattc ggtctctcac gacctacgtg cgccgttgcg tcacatcgac ggctatgccg
     1021 tgctcttgcg taattcggtc gagcattcgc tgaatgacaa ggcagctcgg tatttgcaaa
     1081 cgatttcgga cgccgccaaa caaatgggac agctgatcga cgacctgctg gtgttctctc
     1141 gtatgggtcg gcaagaaatg cttcgcacga ccgtcaacct ggatcaattg atcaatggca
     1201 tcctttccga tcttcgcctg gacttgcaag cacgagcgat atcatggaca atcgctgcgc
     1261 tgcctgaagt acaaggcgat ccggccatgc tccgacaggt atttatgaac ttgatcgcaa
     1321 atgccgtgaa gtttacaagc acgaggccga gcgccgcgat tgagatcggt gtggagcatc
     1381 ccagctctac ggaaatcgtt cttttcgtcc gagacaatgg tgtgggattc gacatgcagt
     1441 atgcccccaa attgttcggg gtgttccaga gactgcaccg agccgacgag tttgaaggaa
     1501 ccggtatcgg cctcgccaac gttcgtcgga tcgttcatcg ccatggggga cggacctggg
     1561 ccgaaggtgt gccggataag ggagcgacgt tctacgtact gctcccggca aggaggcttg
     1621 actcatgacc gtggccaaac cgatcctcct cgccgaggat aacccccgag acgccgagct
     1681 ggcactggcg gcgatggagg cagagcatat ttccgacaaa gtcgttctgt gtcacgacgg
     1741 tgctgaagta ttggattatt tgtattgtcg cggacaattc aagtccaggc tgagaggaaa
     1801 ccctgccgtg gtgtttctcg atctgaagat gcccaaagtg aacggccttg aggtcttgcg
     1861 taccatcaag gcggacgcca atcttcgtcc gattccagtc gtgatgctga catcatcgcg
     1921 agaagaacgt gacctggccg acagctatgc cttgggtgcc aacgcttacg tcgtcaagcc
     1981 cgtggagttt cacaaatttt tatccgcggt caaggagctg ggaacattct ggggtatcat
     2041 caatgaacct cctcctgaaa gctgccgttc cacccaatga ccctgccatg acgactctgt
     2101 tgcgcgtgct gcatatagag attgacccaa acgatactga tcgcatcgag acgctgttga
     2161 ccgatggggg gctttcttgt gcgatacggc gcgtagaaag tcgccatgct tttacttcag
     2221 ctctgaacgg cggccaggtg gatctcatcc tggctgaatt ctccatgcct gagtttgacg
     2281 gggggggcgc cttagagttg gcgcaacagg cggctcccga cgtaccgttt atctttgtct
     2341 cggcatcact tactgagaca gcatgcatcg agcaaatgca gcgcggtgcg acggattgca
     2401 ttcccaagga aagtttggct cgacttgtcc catcggtgcg ccgagtactg cgtgagcagc
     2461 aagcacgagc ggcacgaatg caggcggaga aggcacttct tcaaagcgag atgcaactgc
     2521 ggcagctaca aaaacttgag gccgtcggcc gtctggcggg agggttggca ca
//
LOCUS       DATMTI010000728         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_111865, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000728 DATMTI010000000
VERSION     DATMTI010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111865"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..434
                     /locus_tag="VN039_11650"
     CDS             111..434
                     /locus_tag="VN039_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWV46654.1"
                     /translation="MKNPFGNMNNILKQAQAMQEQMAKIQEQAASKTASGTAGGGIVT
                     VTANGAMQIVSVAIDPEIVKSGDVDMLQDLVVAATNEALRKAKELMEGEMKALTGGMK
                     LPGLF"
     gene            437..1039
                     /gene="recR"
                     /locus_tag="VN039_11655"
     CDS             437..1039
                     /gene="recR"
                     /locus_tag="VN039_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWV46655.1"
                     /translation="MAVDQQGLLARLIKELVRLPGVGHKSAQRLAFHVMKAEREDALR
                     LADAIRAVKDGLAFCRQCRNIAEADLCEFCLDPKRDRTKIFVVEEPSTLYAVERAGAY
                     RGLYHVLLGALSPLDGVGPGDIKADELVERVKFGGVEEIILATNPTIEGEATAIYLTR
                     LLKPFGVRVSRIAYGIPVGMDIEYADEVTLLKSIEGRRDL"
     gene            1128..1577
                     /gene="dksA"
                     /locus_tag="VN039_11660"
     CDS             1128..1577
                     /gene="dksA"
                     /locus_tag="VN039_11660"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HWV46656.1"
                     /translation="MKIRRSGTRTRSTKSQVPVSAKRSVNKPKTGAKNAKYPDIRRDL
                     ERQRAAILNDVGEVLTHRDDLAAFPDVSDQASAEVDQNFSMRIRERERKLLKKIDEAL
                     ERMDAATYGICERCGGDIPYKRLKARPVTTLCIECKTLQEREEQARG"
     gene            complement(1628..>2229)
                     /locus_tag="VN039_11665"
     CDS             complement(1628..>2229)
                     /locus_tag="VN039_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV46657.1"
                     /translation="LFSPYRREFLERLRNPGGGKGGQQMESQGTEDKEPFSSLQQFAR
                     GQAAQLAYCGEKNYDIAADAYQKTIASGFTNKVWLAEAHHKLGLAWEAKGRFDKAKTE
                     MLQSLALRPNIPEVLNNLGTVQVKLGEKAAGLSLFEKAVILRPNYALARYNLAEVSEA
                     TNPKRALSEYETYLAIVEGIPEEADRIAHAKERVKALKQ"
BASE COUNT          539 a          607 c          614 g          469 t
ORIGIN      
        1 ctggtgttgt tcgaacgtgc gaaagcgaac ccgacggtga agcaagccct cgagatattc
       61 ggcgtggaac tggcagaggt tcgtactata acccagcagg aggcaagcga atgaaaaatc
      121 ctttcggcaa catgaataac atactcaagc aagctcaggc catgcaagaa cagatggcca
      181 agattcagga gcaagcggcg tcaaaaaccg ccagcgggac agccggcggc gggatcgtca
      241 ccgtcacggc gaacggggcg atgcagatcg tcagcgtggc gatcgacccc gagatcgtca
      301 agagcggcga tgtcgatatg ctccaagatc ttgtcgtggc cgcgacgaac gaagcgctcc
      361 gcaaagccaa ggaattgatg gaaggcgaaa tgaaagcgtt gaccggcggg atgaaacttc
      421 cgggtctgtt ctagcgatgg ccgtcgatca acagggctta ttggcgagat tgattaagga
      481 actggtccgt ctccccggag tcggtcacaa aagcgcccag cggttggcct ttcatgtcat
      541 gaaggccgag cgtgaggatg ctttacggct ggcggatgcc attcgtgcgg taaaggatgg
      601 gttggcgttt tgcagacaat gtcgcaatat cgccgaagcg gacttgtgcg agttttgcct
      661 cgacccgaaa cgcgatcgga ccaagatctt cgtcgttgaa gaaccgagta cgctgtacgc
      721 cgttgaacga gcaggcgcct accgcggact ttatcacgtc ttgttgggag cgctctctcc
      781 actcgacgga gtaggtccgg gagacatcaa agccgacgaa cttgttgagc gtgtgaaatt
      841 cggaggagta gaagaaatca ttctcgcgac gaaccccacc attgaaggag aagccacagc
      901 gatctacctg acccggttgc tcaagccctt cggcgtacgc gtttcccgaa ttgcttatgg
      961 gattccagtg ggcatggaca ttgagtacgc ggacgaagta acgttgctga aatcgatcga
     1021 ggggcggcgt gatttatagc ccatatagga agacccaacc ccctcgttga cccctctcat
     1081 ttagattggt atagttcgct gttttcctta cacacagaac accagccatg aagatacgcc
     1141 gttccgggac acgcactcgt tcgacaaaat cgcaggtccc ggtgtccgcc aagcgttcgg
     1201 tgaacaagcc gaaaacaggc gccaagaacg caaaatatcc cgacatccgc cgtgacctcg
     1261 aacgccaacg cgcagccatt ctgaatgatg tcggggaagt cctgacacat cgagacgacc
     1321 tcgcggcatt ccccgacgtc agcgaccaag cctccgccga ggtcgaccag aatttttcca
     1381 tgcgaattcg ggagcgcgaa cggaagctgc tcaagaagat cgacgaagcc ctggagcgga
     1441 tggacgccgc cacatacggc atttgcgaac gctgcggcgg agacattcct tacaagcggc
     1501 ttaaagcccg cccggtaacc actctctgta tcgagtgcaa aacccttcaa gaacgggaag
     1561 agcaagctcg aggctgaatc gttttcttca cgcgaatcga aaatgcaaat ataatcccgt
     1621 agacgaacta ctgtttcaat gccttgaccc gttccttggc gtgtgcaata cggtcggctt
     1681 cctccggtat cccctctacg atcgcaagat aggtctcata ttcagaaagg gcacgtttgg
     1741 ggtttgtagc ctcggacact tcggccaggt tgtagcgggc cagagcatag ttaggacgca
     1801 ggatcaccgc cttttcaaac aagcttaaac cagccgcttt ttcccctaat tttacttgga
     1861 cggtgccgag attgttcagg acctccggga tgttaggcct gagggcaagt gattgcagca
     1921 tctctgtttt tgccttgtcg aaccgaccct tggcctccca ggcgagtcct aatttatgat
     1981 gggcttcggc aagccacacc ttattcgtga atccactggc aatcgtcttt tgatacgcat
     2041 cggccgcgat gtcatagttc ttctcaccgc agtaggccag ctgagcagct tggccacgag
     2101 caaattgctg caaagacgag aatggttctt tatcttcggt tccctgactc tccatttgtt
     2161 gtccaccttt gccacctccc ggattgcgaa gccgctcaag aaactcccgc cggtatggag
     2221 agaaaagcc
//
LOCUS       DATMTI010000729         1838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_578869, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000729 DATMTI010000000
VERSION     DATMTI010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578869"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..337)
                     /locus_tag="VN039_11670"
     CDS             complement(173..337)
                     /locus_tag="VN039_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46658.1"
                     /translation="MQQEKERLCKEWGESMLKRYPPESFKDWRPELDLEQARKTARQL
                     GAVIESEPPR"
     gene            complement(337..627)
                     /locus_tag="VN039_11675"
     CDS             complement(337..627)
                     /locus_tag="VN039_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46659.1"
                     /translation="MKPVKKRSRVHIGFPQPWTRAQVEKLNKLVLASEFGEKPVFIRT
                     WPGPALPSEPVPHQCWVRFVELPEVMLVKRDFHAVKFAIEQVPDPSVKRAMV"
     gene            1276..>1838
                     /locus_tag="VN039_11680"
     CDS             1276..>1838
                     /locus_tag="VN039_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46660.1"
                     /translation="MTVGFTALAALSGCFARWFSSRVSALKGESRIHFDTESANTSTE
                     TVSSLEWSADWTGKAAVGFTAAAALSGSLSWFFSWNVSAMNEDARSRFELESKTKMKT
                     AEEGTAKALAEAATAKENTSKLEVEAARLREQAMAAEAKIKAAEARAAEANEQSAKAG
                     EGTAKALAEAAVAKENIGKLAMEAARLR"
BASE COUNT          417 a          525 c          462 g          434 t
ORIGIN      
        1 caattcgtga actcccctgg aaggattcac catcgcatag tggctacaga agggacactg
       61 ccactgaatt agattcgaac cgtcacgctc agggaactct aaaatcatta gcagaccttg
      121 cttctattga ctttccaaac cacctcatcg gtcgacgcgc gcccgggcca gatcatcgcg
      181 gcggctccga ttcaatcacc gctccaagtt gcctcgccgt cttgcgcgcc tgttcgaggt
      241 ctaactctgg acgccaatcc ttaaatgatt cgggcgggta ccgcttcagc attgactcgc
      301 cccactcctt acacaatcgt tctttctcct gctgcatcac accatcgctc gcttcactga
      361 cggatcggga acctgttcga tcgcaaactt gacggcatgg aaatcgcgct ttactaacat
      421 aacctccggc agttcgacga accggaccca gcactggtga gggacgggtt cactcggtag
      481 cgctggacca ggccaggttc taatgaacac cggcttttcg ccaaactcgg aggccagcac
      541 caatttattc aacttctcca cctgcgcgcg cgtccaaggt tgaggaaacc ctatgtgtac
      601 ccgtgaccgt ttcttgaccg gtttcatcag cgacctccgt tgttaactga tgtcatcaca
      661 gctccggcaa cttttcgtgc ggcttcacga ctgctgtggg ccgcacgtcg aaaatctcgc
      721 ccgaccgcat cgtgagctga attgtaaggt atttccgctt tctccgcaat tcacgcagcc
      781 ataagacgaa ctcctaaaaa tcccgatgcg acggccgctg ttcatcagcc acccactgct
      841 ccggccgcgt ttacccccgc cgcctgccgc agttgccgtg ctctgattgt ttcaagctgt
      901 ctcgtggtac tacaatatgg gaccatgctg cctccgacac ctacctcaaa gctcgtctat
      961 cgaaagtgct tcttacacgt aaaatccaaa gtggctctcc cttcatcggg gtactcaaca
     1021 gggcgatgtc aacaatgtgg aattaagaac gctcgtcttt ttcttttgga tatcccttcc
     1081 aagaatgaat gaacgttcgc ctctccgtgg acatttcatg tccacactcc cgagcgtccg
     1141 ttgaattttc ggaacgcccg cttccttttg taattgcttc gcatagcgac tatcattccg
     1201 gtgacctgat ttaagattga aactaacagg atgaatgctt caaccctaga atcatgggca
     1261 ttttggacgg gaatcatgac cgtcgggttt acagcactag ctgcgctgtc tggttgtttt
     1321 gctcgatggt tttcttccag agttagtgcc ctgaaagggg agagccgtat acattttgat
     1381 accgagtcgg ctaacacctc aactgagact gtctcttcac tggaatggtc tgcagattgg
     1441 acgggaaaag cggccgtggg gtttacggcg gcggcggcac tctctggctc cttgtcttgg
     1501 ttcttctctt ggaacgtcag cgcgatgaac gaggatgccc gtagccgttt cgagctggaa
     1561 tccaagacta agatgaaaac cgccgaggaa ggaacggcca aagcccttgc tgaagcagcg
     1621 actgcgaagg agaacacgag taagcttgag gttgaggcgg cccgtcttcg cgaacaagca
     1681 atggcagcag aggccaaaat caaagctgcg gaggcgcgtg cagctgaagc gaacgaacag
     1741 tctgcgaagg ctggagaagg aacggccaag gccctcgctg aagctgctgt tgcaaaagaa
     1801 aacatcggta aactggcgat ggaggcggct cgtcttcg
//
LOCUS       DATMTI010000730         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_494963, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000730 DATMTI010000000
VERSION     DATMTI010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494963"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..192)
                     /locus_tag="VN039_11685"
     CDS             complement(16..192)
                     /locus_tag="VN039_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46661.1"
                     /translation="MWNVEETSSLHYGTAYLVARRGMVLGSSDGRGSGGALHFRVKSD
                     AEEIAALLNMAECK"
     gene            complement(198..731)
                     /locus_tag="VN039_11690"
     CDS             complement(198..731)
                     /locus_tag="VN039_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46662.1"
                     /translation="MKTGTIVQQRGIPMPPHMVNPAPLPPALEGLFDLRAWFESLARK
                     VEYKPLNPDYMAERMLALTLMSGSVEELLSPKNLTGLQDLIPNTPGATSGPIRITEIY
                     VDKSDQEDGNRCYILFSFINLETGYEVTTTTGATQVQAVFLTWLALGQWPIECRITRG
                     DRTDRGGRYMFLIQPVE"
     gene            complement(908..1831)
                     /locus_tag="VN039_11695"
     CDS             complement(908..1831)
                     /locus_tag="VN039_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46663.1"
                     /translation="MNSGESSDRGEEIQRLYLAAYQAGLATDRDRVARNLAWYRRWYR
                     GHISLGQLMGRTGVKMRPKITVQRRVDMTSDGTTVMVDTIHDAVAHVPTGIHYVGGYV
                     SGTPDIKWTPEDWARFPSARHIRIFQGYGDMPDVHGFDAIDVETGAVTPGEAASIVRT
                     RVMAGIPWTHIYGTDSTLATVGEAIRAYGQSIWNGHVSCWLADWNLDESEAAAKVGTR
                     IHGMTCVAVQWASPSSNPHTMLPGTQLTLREADCDLSVVDATWIPSGGWGLPPKPVAP
                     PTIVTQHGVLVQLPGGHSVVVESIDGGKTWH"
     gene            complement(1818..2165)
                     /locus_tag="VN039_11700"
     CDS             complement(1818..2165)
                     /locus_tag="VN039_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46664.1"
                     /translation="MSETDQAPARSYGCSNGCGNPYDFVVITVSDAFTQMLCFPCFVG
                     AAAVMVKAATEPNDETVRSAVAEFPPAEQVPMRGGVTRTRGHHAPAESDDPDALEVFD
                     GIITEDDLPDEFR"
     gene            complement(2162..2320)
                     /locus_tag="VN039_11705"
     CDS             complement(2162..2320)
                     /locus_tag="VN039_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46665.1"
                     /translation="MTPQGYRFVTISTAPGRRVGIMVCKQGATIEESIKAALDTVALL
                     PGVSEVKS"
BASE COUNT          459 a          748 c          690 g          496 t
ORIGIN      
        1 gtacgactac gaatctcact tgcattccgc catgttgagc agtgccgcga tttcctcggc
       61 atcactcttc actcggaagt gcagcgcgcc accggatccg cgcccgtccg atgagccaag
      121 caccatgcca cgcctggcca ccaaataggc tgtcccgtag tgcagtgagc tcgtctcttc
      181 cacgttccac atgccgatta ctcaaccggc tggatcagga acatgtaccg gccgccacgg
      241 tcggtacggt cgccgcgcgt gatgcggcat tcgatgggcc actgtccgag tgccaaccag
      301 gtcaagaaaa ccgcctgaac ctgcgtcgcg ccggtggtgg tggtgacctc gtatccggtc
      361 tccaggttga tgaacgagaa caggatgtag cacctgttcc catcctcctg atccgactta
      421 tccacgtaga tttccgtgat gcggatcggg ccggacgtgg caccgggcgt gttcgggatg
      481 agatcctgca agccggtgag gttcttcggc gacaggagct cttccaccga tccggacatg
      541 agcgtgagcg ccaacatgcg ctccgccatg tagtcggggt tgagcggctt gtactcgacc
      601 ttccgcgcga gcgattcgaa ccacgccctg aggtcgaaga gaccttcgag tgcgggcggg
      661 agcggcgccg ggttgaccat atgcggcggc atgggaatgc cgcgctgctg tacgatggtc
      721 ccggtcttca cttcaacttc cttgggcacc atataccctt tcatcattat cagctgtgtg
      781 cgatgttgca ttccgtcgta tgacgggtgc ggtattcggg tcagactcgg cttgttaacc
      841 cgcgcatggc ccgaatgcca cactcgctac acgatgagat agtcggatcc ccgcgtatag
      901 cgtagatcta gtgccaggtc ttgccgccat cgatggactc gaccaccacg gagtggccac
      961 ccgggagctg caccagcacg ccatgctgcg tgacgatggt cggcggcgcg accggcttcg
     1021 gcggaagtcc ccagccaccg gacggaatcc acgtcgcgtc cactacggag aggtcgcaat
     1081 cggcctcacg gagtgtcagc tgtgtaccgg gaagcatcgt gtgcggattg ctcgatgggg
     1141 atgcccattg cacggccacg cacgtcatgc cgtgaatccg tgtaccgacc ttggcggctg
     1201 cctcggattc gtctagattc cagtcggcaa gccagcatga cacgtgtcca ttccatatgg
     1261 actggccgta tgcccttatg gcctcaccga ctgtggcaag cgtcgaatcg gtcccataga
     1321 tgtgtgtcca tgggataccg gccatgacgc gagtgcgcac gatgctagcc gcttctccgg
     1381 gtgtcacggc gccggtttcg acgtcgatgg catcgaagcc gtgcacgtcc ggcatgtcgc
     1441 cgtatccctg gaatatccgg atgtggcgcg cgctggggaa gcgtgcccaa tcctccgggg
     1501 tccacttgat gtcgggggta ccggatacgt acccgccgac atagtgaatg ccggtgggca
     1561 catgcgctac agcatcatgg atcgtgtcca ccatgaccgt agtgccgtca cttgtcatat
     1621 ctaccctcct ttgcactgta atcttcgggc gcattttcac gcctgtgcgt cccatgagct
     1681 gacctagcga gatgtgaccg cgataccaac ggcggtacca agcaagatta cgagccaccc
     1741 ggtcacgatc agtggcaaga ccggcttgat aagccgccag atagaggcgt tggatttcct
     1801 cgccccggtc cgatgactca ccggaattca tccggcaagt catcctccgt gataatgccg
     1861 tcgaagactt ccagcgcatc cgggtcatcc gactcagctg gcgcgtggtg tccgcgcgtg
     1921 cgcgtcactc cgcccctcat gggcacctgc tcagccggag ggaactccgc gacggccgat
     1981 cggaccgtct cgtcattcgg ctcggtagcc gccttgacca tgacggccgc tgctcccacg
     2041 aagcagggga agcacagcat ctgcgtgaat gcgtcggata ccgtgatgac aacaaagtca
     2101 tacggattgc cgcacccatt ggagcagccg taagatctgg caggcgcctg atctgtctcg
     2161 ctcatgattt cacctcactc acacccggta atagcgccac tgtatcaaga gccgccttaa
     2221 tggactcttc gatcgtggcc ccttgcttac ataccataat gcctacccgc cgaccgggcg
     2281 ccgtcgaaat ggtcacgaaa cggtaaccct gcggcgtcat tgtaacactc aatacgatgg
     2341 gatcgtatga tggcatatcg catcatccca tcatgtcatc ggaacttttc gaa
//
LOCUS       DATMTI010000731         1865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_241026, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000731 DATMTI010000000
VERSION     DATMTI010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241026"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>1865
                     /locus_tag="VN039_11710"
     CDS             29..>1865
                     /locus_tag="VN039_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46666.1"
                     /translation="MVGLKTMGISCVAAGFALLAALATGGAKAQSIVQPPVASIIDGN
                     GVNLATGKLQLPNLDLGIGGNGSGLARSTNSFLSDTTSTAGTADNFSGSMVVSAPPGG
                     TGAAFTYNISYGGKVYRFLLYYTNGAWLPPQSADGGPQQLNCSDPTPTNGVGTCDLLL
                     EDGTVAHYDEAIGSYLTSITRPNGEVISLTYYQSSGVKAIKSVSSSLGWMLKYEVDGS
                     YKVIKLTGINTAVTYCDPNANSCSVGGASPTESLTVSGSTTTLTRNGVTILNYVSSAS
                     GATFTSPNNVSKTVTLYTSGTDNGRVHQVTSGGSTWTYSYVTYAGSGLQTTVQAPNGT
                     SRQTFVDINGKTQWQKDEEGRTTTYVYSTASGAAFGQLVKIVAPDGDGSTGGFTAFSY
                     DSLGRLTMKQVVAKGGASNGIPIAGGALVTSYTYANCDGTNDKWCRKPTAVTDENGVT
                     TNYAYDSYSGEVATALFPAPASGQARPKITYSYSAFTPYVKNSSGALVAQGAVYRLTE
                     TSTCRTADNGNCINGGDEKQTIISYAGSNNLLPTSTTVKIGDGSYPQTTTATYDDNGN
                     VIVADGPKSGAVDETYTFYDTLGRVQGAVGVDPDGASGSRHRQASH"
BASE COUNT          437 a          501 c          532 g          395 t
ORIGIN      
        1 atacaggcag gacaatcggg ggtagggcat ggtcggcttg aagacgatgg gtatcagctg
       61 cgtggccgct gggtttgcgc tgttggcagc cctggcgacc gggggcgcca aggctcagtc
      121 gattgtacag cctcccgttg catcgattat cgatgggaac ggcgtcaatc tcgcaactgg
      181 caagctgcaa ttgccgaacc tcgacctcgg aattggcggc aatggcagcg gcttagctcg
      241 aagtaccaac agtttccttt ccgacacaac cagtacggcc ggaacagctg ataacttttc
      301 aggctcaatg gttgtgtccg cgccgcccgg cggtactggg gcagcattta cttacaatat
      361 cagttacggc ggcaaggttt accgcttttt gttgtattac actaatggag cttggctacc
      421 ccctcagtct gcggatggcg gaccgcagca gcttaattgc tcggatccga cgccgacaaa
      481 tggggtaggg acatgcgatt tactcctgga agacgggacg gtcgcgcact atgacgaggc
      541 cattggttcc tatctgacgt cgataactcg accgaatggt gaagtgataa gcctgaccta
      601 ttatcaatct tccggcgtta aggcgatcaa atccgtcagt agtagcttgg gttggatgct
      661 taaatatgag gttgatggct cgtacaaggt gattaagctg acgggcatca atactgccgt
      721 cacctattgc gaccccaacg caaattcgtg ttctgtcggc ggcgcctctc caaccgaatc
      781 attgacagtt agtggttcga ccacgacgct gacgcgcaat ggcgtgacca ttctaaacta
      841 tgtgtcaagc gccagcgggg cgacctttac atctcccaac aatgtctcaa aaaccgtcac
      901 actatacacg tccgggaccg ataatggacg cgtccaccag gtgacctctg gcggttcgac
      961 gtggacctat tcctatgtga cctatgccgg cagcggacta caaaccacag tccaggctcc
     1021 gaacggaaca agccggcaaa ccttcgtcga catcaacggc aagactcagt ggcaaaagga
     1081 tgaggaaggg cggacgacaa cttatgttta tagtacggcg tcgggcgcag cctttggtca
     1141 attggtgaaa attgtcgctc ctgacggcga tggttcaaca ggtggcttta ccgcttttag
     1201 ctatgacagc ctcggccgct tgacgatgaa gcaggtcgtc gcaaaaggag gggctagcaa
     1261 cggtatccca attgcgggcg gcgctcttgt caccagttat acttatgcaa attgcgatgg
     1321 tacgaacgac aaatggtgtc gtaagcctac ggcggtaaca gacgaaaatg gcgttacgac
     1381 aaactatgct tatgattcct acagcggtga ggtggcaacc gccctgtttc cagcaccggc
     1441 gtccgggcag gcgcgcccga agatcaccta tagctacagc gcctttacgc cctatgtgaa
     1501 aaacagctcc ggcgcactag tggcgcaagg ggccgtttac cgcctgaccg agacgtcgac
     1561 atgtcgtaca gccgacaatg gcaattgcat caatggcggc gacgagaagc agaccattat
     1621 cagctatgct ggcagcaaca atctgctccc gacatcgacg acggtgaaga tcggggacgg
     1681 aagctatcct caaacgacga cagcgaccta tgatgataac ggcaatgtca tcgtcgctga
     1741 cgggcccaag tccggcgcgg tcgacgagac ttacaccttc tatgatacgt taggacgggt
     1801 tcagggtgcg gtcggcgttg atccggacgg cgcttcgggt tcgcgccatc ggcaggccag
     1861 tcaca
//
LOCUS       DATMTI010000732         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_241060, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000732 DATMTI010000000
VERSION     DATMTI010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241060"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..306
                     /locus_tag="VN039_11715"
     CDS             145..306
                     /locus_tag="VN039_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46667.1"
                     /translation="MAVARSIRAEETTQRSAFMRRRELRMLVQRASFIEWEAMLDERA
                     QEKPLAVYE"
     gene            299..607
                     /locus_tag="VN039_11720"
     CDS             299..607
                     /locus_tag="VN039_11720"
                     /inference="COORDINATES: protein motif:HMM:NF020529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1871 family protein"
                     /protein_id="HWV46668.1"
                     /translation="MNESKKESKQKYKQMFSTIKVVIDRWDPYGLLEGGAPEDEFESE
                     IALVVAKIKTIKTEDDAARVVSEVFSDAFEPDLFTMESCASVGRELFQELKKAKFFLN
                     "
     gene            628..1077
                     /locus_tag="VN039_11725"
     CDS             628..1077
                     /locus_tag="VN039_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV46669.1"
                     /translation="MPAHTIRQATLDDLDQLIPLFDAYRRFYGQQSDDIVARQFLNDR
                     LTRNESIVLIAENDSGAAVGFVQLYPTFSSILAAPMYVLSDLFVIPEARRRGVGTQLL
                     ASAAEAARTAGAVRLELPTAITNISAQRLYEALGWRKDDFYLYGISL"
     gene            complement(1091..1327)
                     /locus_tag="VN039_11730"
     CDS             complement(1091..1327)
                     /locus_tag="VN039_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46670.1"
                     /translation="MLDSHCCAPNCLEASYAPAGTRSICKDHFLNFLTWRRRRGVQMF
                     HTYAGMTMEERDTIAAEWMKTIRTDELPAPAPKL"
     gene            1437..2918
                     /locus_tag="VN039_11735"
     CDS             1437..2918
                     /locus_tag="VN039_11735"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HWV46671.1"
                     /translation="MHAFLLLAGILVLGSSFVGCVVFSNESVRSSSSFEGKPFIDSDF
                     LPQGVAVGDVGSQSALLWLRTDGPASVQIEWATVSRWERASKLATVVAPVPRTASIMT
                     TAETDYTLIIPLEGLIPATRYRYHVLVGSVDQNARRLSASLAAMGEFTTLPDDKTSAA
                     VTFVWSGDLGGQKRCRQGVGGYPIFDIIRRQHPDFFLFLGDTIYSDDICPSPPNEPGA
                     DFKATTLQTYRVRHRYQRGAEALRRFLETTPVYVVWDDHDVRNNFSGPYDEQMPAGRQ
                     ALREYWPIASPAHDPHRLYRHVRYGADLELFILDTRQYRSRNADPDGPDKTMLGATQL
                     AWLLDGLRTSTATWKAIVTSVPLSMPKGGDVNVPGYDGWAGGLDGTGFERERRVIVDT
                     ILDHKLKNVVFLAGDVHLAQANAYDPDRDGVIDFHEYVVGPLSARHGRLTATDDGLHQ
                     TQLFYESGFDNFGLARVTKNEFAIAVVDEAGTKRFSHVVPARP"
     gene            3028..3510
                     /locus_tag="VN039_11740"
     CDS             3028..3510
                     /locus_tag="VN039_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46672.1"
                     /translation="MSGLMSADDMFSKAEAAAVAATGPDEKALQIDYDALREKFRKAL
                     GDRKVALCHVNKFLPEGYEDQGRFNLIVLTAVNVVFDIVIGDSYFRYDVVSVGQLDKV
                     QLIDAMWDNKEKRREEPFLSLRLMHGDEAHLLLALDENERASLLKIASAVTAARNPER
                     "
     gene            complement(3559..4071)
                     /locus_tag="VN039_11745"
     CDS             complement(3559..4071)
                     /locus_tag="VN039_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46673.1"
                     /translation="MVGSVLALVLVFSIVSIRGKPPQPADGTRVTVQEATIVGPLVQH
                     NIPTATVLLKNTGHSPVLTTKARLVMTVWTANTFPDWDMPLKLTPDAETIGEIGPESV
                     VPRTVSLIAPLTDVQGMHLERKDWFIVILGIVSYVDILGNPHETKLCLIWRETSDEHL
                     SLCERWNATD"
     gene            complement(4229..>4460)
                     /locus_tag="VN039_11750"
     CDS             complement(4229..>4460)
                     /locus_tag="VN039_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46674.1"
                     /translation="EPLNLGDTINTAGEDMCWTFTPDGQTFSGGTGPRGSYNHDIMWV
                     HKDQVPLLKNFEPIGPPPNLLVNSKVSSGEPK"
BASE COUNT          963 a         1146 c         1299 g         1052 t
ORIGIN      
        1 aagagcgctc gctcaatgcg cggccatcgg gaaagcagac acttcggtgt ctgtggggtg
       61 ggcggatgag atcggcggcc ctcgttggat gcgcgcagtt aggagaccac taggccgccc
      121 gcttgagaga gaaggaaaag agaaatggca gttgctcgct caatacgcgc agaagaaacc
      181 acacaacgct ccgcctttat gagaagaagg gaattaagaa tgctcgtcca acgcgcaagt
      241 tttatcgaat gggaagcgat gctcgatgaa cgtgcgcagg agaaaccgct agctgtctat
      301 gaatgaatct aagaaggaat ctaagcagaa atataagcag atgttttcca cgattaaggt
      361 tgtgatcgat agatgggatc catacggatt acttgaaggt ggcgcaccag aggacgaatt
      421 cgagagtgaa atcgcattag tagtggcaaa aattaaaacg attaagacag aagatgatgc
      481 ggcaagagtg gtttccgagg tcttctctga tgcatttgag cctgatttat ttaccatgga
      541 atcctgtgct tcggtagggc gtgagctgtt ccaagaatta aagaaagcta aatttttcct
      601 gaactgattc ttgttccaag agcgctcatg ccggctcaca ccatccgtca agcaacgtta
      661 gacgacctcg accagctcat tcctctcttt gacgcctacc gccggttcta tgggcagcag
      721 tcggacgaca ttgttgcccg gcaattcttg aacgatcgtc ttacgcggaa tgagtcgatt
      781 gtcttgattg ctgaaaatga tagtggagcg gcggtcggct ttgtgcaact gtaccccact
      841 ttttcatcca ttctcgccgc accgatgtat gtgctcagcg acttgtttgt catacctgag
      901 gctaggaggc gaggcgttgg aactcaattg ctcgcgtctg ctgccgaggc cgctcgcacg
      961 gccggcgcgg ttcggttgga actgccgacc gcgatcacca atatttcggc acaaaggctc
     1021 tatgaagcgt tggggtggcg gaaagacgac ttctacctgt atggcatatc gttgtagcag
     1081 agcgacgagg tcagagtttc ggtgcagggg cgggcagttc atccgtccga atggttttca
     1141 tccattcagc ggcgatggta tcgcgttcct ccatcgtcat gccggcgtag gtgtggaaca
     1201 tctgaacgcc acgtcggcga cgccacgtga gaaagttgag gaaatggtct ttgcagatgg
     1261 aacgcgtgcc ggccggtgca taggatgcct ccaggcaatt aggcgcgcag cagtgactat
     1321 ccaacatctt ggtcctccgg tgaacttgtt cagtatgccg gtcggctgtg ccgatttcaa
     1381 gcccgcgacg ccttcgctgc ttgaccccgt cagcttcata gggtaggact gccccgatgc
     1441 acgcgttcct tttgctggcc ggtattctag tccttggctc ttctttcgtc ggctgcgttg
     1501 tattttcaaa tgagagcgta cgatccagca gttcgttcga gggaaagccg ttcatagact
     1561 cggacttctt gccccaaggt gttgcggtcg gcgatgtcgg ttcgcaaagc gcgttgctct
     1621 ggttgcgtac cgacggacct gcctcggttc agattgagtg ggccactgtt tcacgatggg
     1681 aacgagcctc aaaacttgcc acagtcgtgg ctccggtgcc gagaacggcg tccatcatga
     1741 cgaccgcaga gacggattat accctcatta tcccattgga aggtctgatc cccgccacgc
     1801 gctatcggta tcatgttctg gtcggttctg tcgaccagaa cgcaagacgg ctctctgcaa
     1861 gtcttgcggc catgggcgaa tttaccacct tgccggatga taagacttcg gctgcggtga
     1921 cgtttgtctg gagcggagat cttggtggtc agaagcgctg tcgccaaggt gtcggcggct
     1981 atccgatctt cgacatcatc cgtcggcaac atcccgactt ctttctgttt ctcggggata
     2041 cgatctatag cgacgacatc tgtccctctc cgccgaacga accgggtgcg gacttcaaag
     2101 cgactacgct gcagacctat cgggtacgtc accgttatca acgtggtgcg gaggctctgc
     2161 gccgattctt ggaaaccacg ccggtctatg tggtgtggga tgatcatgac gtgaggaaca
     2221 atttttccgg cccgtatgac gaacagatgc cggccggccg gcaggcattg cgggagtact
     2281 ggcccatcgc ttctccggca cacgatccgc atcgtctcta tcggcatgta cgttatggtg
     2341 ccgacctcga actctttatt ctggatacca ggcaatatcg gagccgtaat gccgatccgg
     2401 atggtcctga caagaccatg ctcggagcga cacaattggc gtggttgctc gatggccttc
     2461 gcacttcaac tgcgacctgg aaagcgatcg tgacctccgt tccgctctcg atgccaaaag
     2521 ggggtgatgt caacgttccc ggctacgatg gctgggcggg agggctggac ggcaccggct
     2581 ttgaacggga acgtcgagta atcgtcgata ctattctgga tcacaagctg aaaaatgtgg
     2641 tgtttcttgc gggtgatgta cacctcgcac aggcgaacgc ctatgatccc gaccgggacg
     2701 gcgtgatcga ttttcacgaa tacgtcgtcg gacctctttc cgcccggcat gggagattga
     2761 cggcgacgga tgacggactg catcagacgc agctctttta cgagtccggg ttcgacaatt
     2821 tcggtctcgc tcgcgtcacc aagaacgagt tcgccatcgc agtggtggat gaggccggta
     2881 cgaagcgatt ttctcacgtt gtgccggcca ggccgtaggc cgttctcacc gttcaagcca
     2941 cttgcaattg cggcagatgg ccggttaccg tacggctccg gaggtcattg ctcagttcac
     3001 tgcaacgata gcaagggagt acacgccatg tcaggattga tgtccgccga tgatatgttc
     3061 tcgaaagcgg aagcggcggc cgtagctgcg actgggccgg atgaaaaggc cctgcaaatc
     3121 gattatgacg cattgagaga gaaatttcgc aaggcgttgg gtgatcgaaa agtggcgctt
     3181 tgccacgtca ataagttttt accggaagga tacgaggatc aaggtcggtt caacctgata
     3241 gtactgaccg ctgtcaatgt cgtgttcgac atcgtcatcg gtgattccta tttccggtac
     3301 gatgtggtat cggtcggtca attggacaag gtgcagctga tcgatgcgat gtgggacaac
     3361 aaggagaagc gccgcgaaga gccgttcttg agtctgcgtc tcatgcacgg agacgaggcc
     3421 catctgctgt tggcgcttga tgagaatgaa cgggcaagtc tgctgaaaat tgcctcggcc
     3481 gtcaccgcgg cacgcaatcc tgagcgctga gcgtagacag gtttagagaa cattcggatg
     3541 gttcgttcat agaccccctt agtccgttgc attccatctc tcacagaggc tcaggtgttc
     3601 gtcagaggtc tctcgccaga tgaggcatag cttcgtttca tgaggattcc ccaaaatatc
     3661 gacgtacgac acgattccaa gtatcacaat gaaccaatct ttgcgctcca aatgcatgcc
     3721 ctgcacgtcg gtgagcgggg cgatcaaaga aacggtccga ggcacgacgg attccggacc
     3781 gatttctccg atggtttcgg catcgggcgt cagtttcaga ggcatgtccc agtccgggaa
     3841 tgtgttggcg gtccagacgg tcatcacgag acgggctttg gtcgtcagta ctggagagtg
     3901 cccggtattt ttgaggagca ccgttgccgt tgggatgttg tgctggacga gggggccgac
     3961 aatcgttgct tcctgcaccg tcactcttgt cccgtcagca ggctgaggtg gcttccctcg
     4021 tatcgacaca atactgaaaa ctaggacgag ggccagaact gaaccgacca aaaggtacct
     4081 atgcttttct aggaaaccct tcgagcttga ttgctcgaag ggtgcttctt cctggtcggc
     4141 gctgggctct ggcctagaag ggttggtcat tcagtcggga gaagcctctg agtgagaaaa
     4201 ccgtccttcc tcagcagata caccacgatc actttggttc gccggacgac accttgctgt
     4261 tgacgagtaa attaggtggt ggtccgatcg gctcaaaatt cttcagcagc gggacctggt
     4321 ctttgtggac ccacatgatg tcgtgattat acgaaccgcg agggcccgtg ccacctgaga
     4381 atgtttggcc gtccggcgta aaggtccagc acatatcttc accagctgta ttgatggtgt
     4441 caccgaggtt cagcggctct
//
LOCUS       DATMTI010000733         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_27793, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000733 DATMTI010000000
VERSION     DATMTI010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27793"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3357
                     /locus_tag="VN039_11755"
     CDS             <1..>3357
                     /locus_tag="VN039_11755"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46675.1"
                     /translation="FLLAHYYPPSNTWNYSAYPLPELGGGAAVLTCDGSQSDVISSRA
                     GSCTLILEDGTRVLYDRSLSSGTSFGPMTSVTKPSGEVITFNYYTVTTNGVTIAKAVH
                     TVNSSLGWALKYTVDANYAVTGVTAVNTSVVRCDAAVCSPGGSYPTVTKSVNGATTTL
                     SRNGVNMLSYSVSGGVTTLTSPNGVTKTINTYTSGAFNGRVSSVAVGGQTWGYAYSSD
                     ANTGETTTTVTEPNGSTHKLVISYNNTIKSMTDEAGRTTTYLYDSNFHLVKTISPDGD
                     AMTGGFTTIAYDSIGRVTSQQVVAKGGASNGVPNASASLVTSYAYANCDGTNDKWCRK
                     PTSVTNANGVTTTYTYDSASGQVATTMLPAPGSGGARPKVTYSFTNYSPQNYDVPGYE
                     GSLHTVLNPIRLVTGTSTCRTADNGNCVNGGDEKQTLISYAGSNNLLPTSTTVKIGDG
                     TYPQTTTATYDDNGNVIVADGPKSGAVDETYTFYDTLGRVQGSVGVDPDGASGSRHRQ
                     ASHTYYDNDGHVKEVDTGIVGAGNSDAYSGSTAAARWSQAQSDWAGLTILERNTNEFD
                     GYGRPVVARRYIGTSSTEKDVTQRSYDSMQRLDCEAVRLNPVNFSVLPTACSQGNVTS
                     DGLHDRITKYAYDLLGHMVSTTSAYGTSLARTDASKVYDIGSSTSTGTLQYVMDAKGN
                     KTSYYYDGFNRLIKTCYPTPSNGGTTNTSDCDQTVYRTTLVDNTTTQATSLADHVVLR
                     DGQTISFAYDLLGRVSAKSGAVSESYAYDNYNDITTHVNNTTGGTSASETYGYNALGW
                     LLSDAQPIGTVSYGYDAYGRRNNMTYPDGFYVTYQYWNDDSGWYIQDNTGEYILRHEY
                     DDYGRRLNKKLSNGSPYALLQSSSFDTNLRLQTLTNNLSSSGATAYDNAYTFGYNSAD
                     QLASSGGSNSSYDYQPSSNSISYGVDGLNRIASVNNTGFSYDGRGNLTSDGGSGTYSY
                     NANNLMTSAVQSGVTTSMTYDAENRLASITKNGTTTKFLYDGADMIAEYDGSSLLRRY
                     VHGFDVDEPVVSYDGSSTADSNRHYLVADVRGSIVAVTDSAGNVGASGTGVNTYDAYG
                     LPNTGNTGRFQYTGQVWLPEIGMYYYKARLY"
BASE COUNT          800 a          959 c          922 g          676 t
ORIGIN      
        1 tttttgctgg ctcactacta tccgccctcc aacacctgga attacagcgc ttatccgtta
       61 cccgagcttg gcggcggcgc ggcggtcctg acgtgcgacg gttcgcaatc tgacgtgatt
      121 tcaagtcgcg ctggctcgtg cacgctgata cttgaggacg gcactcgagt tctctacgac
      181 cgcagccttt cgtcggggac gtcgtttggg ccgatgacgt ctgtgaccaa gccgagcggt
      241 gaggttatta cgttcaacta ttacaccgtg acaacgaatg gcgtgacaat cgccaaggcc
      301 gtccacaccg tcaacagttc cttgggctgg gcgcttaaat atacggtcga cgccaattac
      361 gcggttaccg gcgttacggc ggtgaataca tccgtagttc gttgtgacgc tgccgtttgc
      421 agtccaggcg gcagctatcc tacggtgacg aagtctgtca atggggccac aacgacgttg
      481 tcgcgaaacg gcgtcaacat gttgagctac tcagtgtctg gaggtgttac gaccctgaca
      541 tcgcctaacg gcgttacaaa gacgatcaac acctatacgt cgggtgcgtt taatggtcgt
      601 gtttcctccg ttgccgttgg aggacaaacc tggggctatg cctatagctc agacgcaaat
      661 acaggcgaga cgaccaccac ggtgacagag ccgaacggca gcacgcataa gctcgtcatc
      721 tcctacaaca acacgatcaa atcgatgacg gacgaggcgg gccggacgac cacctacctg
      781 tacgacagca acttccacct cgtaaaaacg ataagcccgg atggcgacgc aatgacgggc
      841 ggctttacga cgattgctta tgattccatc gggcgcgtta caagccagca ggtcgttgca
      901 aaaggagggg caagcaacgg cgttcctaat gccagcgcct cccttgtcac cagttatgct
      961 tatgccaatt gcgacgggac gaacgacaag tggtgtagaa agcccacctc cgtcaccaat
     1021 gcaaatggcg tgacgacaac ctacacctac gatagcgcca gcggccaggt cgcaacgacg
     1081 atgcttccgg cgccgggctc aggcggcgcg cgaccgaagg tcacctacag ctttaccaac
     1141 tattccccgc aaaactacga tgtgcccgga tatgagggca gtcttcacac ggttctcaac
     1201 cccattcgcc tggtgacagg cacatcgacc tgccgcaccg ccgacaatgg caattgcgtc
     1261 aatggcggcg acgagaagca gaccctaatc agctatgctg gcagcaacaa tctcctcccg
     1321 acatccacga cggtgaagat cggggacggt acctatcctc aaacgacgac agcgacctat
     1381 gacgataacg gcaatgtcat tgttgctgat gggcccaagt ccggcgcggt cgatgagact
     1441 tacaccttct atgatacatt aggacgggtt cagggctcag tcggcgttga tccagacggt
     1501 gcgtcaggct ccaggcacag acaggccagt cacacctatt acgacaatga cggccacgtg
     1561 aaggaggtcg acacgggcat tgtcggcgcc ggaaatagcg acgcctattc cggttcgacc
     1621 gccgcggcgc gctggtcgca ggcgcagtca gactgggccg gtctaacaat tttggagcgc
     1681 aacaccaatg agtttgacgg ctatggccgc ccggtcgtgg cgcggcgcta tattggaacg
     1741 tcatcgacgg agaaggacgt cacccagcgc tcatacgaca gtatgcagcg cctcgattgt
     1801 gaggccgttc ggcttaatcc cgtcaacttc agcgtactgc ccacagcttg tagtcagggt
     1861 aacgtcacaa gcgacggcct ccatgatcgt atcaccaaat acgcctacga tctgctcggc
     1921 cacatggtca gcacgaccag cgcctacggt acgagtctag cccgaaccga cgcgtcgaaa
     1981 gtctacgata tcggttcatc gacaagcacc ggaacactgc aatatgtcat ggacgccaag
     2041 ggcaataaga ccagctatta ttacgacggc ttcaatcgcc tgatcaaaac ctgttacccg
     2101 acgccgagca atggcgggac gactaacaca agcgattgcg atcagacggt ctatcggacg
     2161 acgcttgtcg ataacacgac gacgcaggcg acgagcttgg ccgatcatgt ggtcttacgc
     2221 gatggtcaga cgatctcatt tgcgtacgat ttgctgggtc gggtcagcgc caagtcgggt
     2281 gctgtctcgg agagctacgc ctacgacaat tacaacgaca tcactaccca tgtgaacaac
     2341 acgacgggtg gaacctcggc cagcgagacc tatggttata atgccttggg ctggctcttg
     2401 agcgatgctc aacccatcgg caccgtcagc tatggctacg atgcttacgg tcgtcgaaat
     2461 aatatgacct atcccgacgg tttctacgtc acctatcaat attggaatga cgacagcggt
     2521 tggtacattc aagataacac cggcgaatat attcttcgtc acgagtacga cgattacggc
     2581 agacgcctta acaagaagct gagcaacggg agcccttacg ccctccttca atcatcaagc
     2641 ttcgatacga atctgcgcct gcaaaccctc acgaacaatc tgagttctag cggggcgacg
     2701 gcctatgaca acgcctatac atttggatac aattccgcag atcaactggc gtcgagtggc
     2761 ggctcaaact ccagttacga ttaccagcca tcgtccaact ccatcagcta cggcgtcgac
     2821 ggtctgaatc ggattgcttc ggtgaacaac accggtttct cttatgacgg tcgggggaac
     2881 ctgacaagcg acggcgggag cggaacctat agctataacg ccaacaatct gatgacgtcg
     2941 gctgtgcaat ctggcgtgac aacgagcatg acctacgacg ctgaaaaccg gctggcaagc
     3001 atcacgaaaa acgggaccac gacaaagttc ctgtacgatg gcgccgatat gatcgcagaa
     3061 tatgacggct cgagcctgtt gcgccgttat gtgcatggct tcgacgtcga tgaaccggtt
     3121 gtcagctatg acggttcaag cacggcggat tccaatcgtc actacctcgt cgccgacgtg
     3181 cgcgggtcga tcgtggctgt gacggattcc gcggggaatg ttggggcgtc aggaacgggc
     3241 gtcaatacct atgatgcgta cgggcttccg aatacaggga atacgggcag gttccagtac
     3301 acaggccagg tctggctgcc tgagatcggt atgtattatt acaaggcgcg gctttac
//
LOCUS       DATMTI010000734         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_281920, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000734 DATMTI010000000
VERSION     DATMTI010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281920"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2897)
                     /locus_tag="VN039_11760"
     CDS             complement(<1..2897)
                     /locus_tag="VN039_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46676.1"
                     /translation="MPTNVITISEAGVVTDYTRYIYGGVQKTDNAADPNLLDFSLFSY
                     NRKVSMPKAGAYVTITTATYGAWFTGYIITDPEVTYIGEQRTVGPRWAWKYQATSDEL
                     VLNKHPLGIIPPFINQTQGQILKQLVAILSPATVSFNVSGIQDGMLLPRYVVDPNQHF
                     SDVVKSFAQSANFRFSCVSRAMTFAPKDSTPLTITVDGANVNFTPGSLRLQPVADSIV
                     NQAIVLGNIEPQNYMNEFFVGDGATAKFALISSVYGADSATLIDDSFTNNTIDDSKWT
                     VYDPTGTVLFPFGGTINAVGGSNNRSYDVSLQSLNLVPLEGTLRLTHGEWDFVQFSSG
                     IIGGLFTGNVTSDLSSLLYGISVDTWRQVNNVWTQISPTTATATDTFNLHPIVNGQVD
                     MNQWAPIDTSKRYIMRTLFATQRSMRQAGQWSFVDATGNVVQIPGGADADTAYFSTYI
                     SEVDPNTGQITNFWVFNNTGVSLSSQQTYAYYCPLVVDYCNLTFTSVTVSCPIMAQLR
                     QAARGSLNWVPAPVGPNEIDSYDGLAPVATIVDSNQGATTKSSLLGTRNYNPGQASLQ
                     YFSDQPVPIPANTLAAINPDLYAVPNPIRIPWGSTSGNTTVSWANAPSASMELWINGA
                     GVSPGTKVASGGQTGSYSATGVSNGTTFYLMDVSGGHPGNLVVYLTIETEIMYIPQPG
                     DLVWLQYRSAGAAIGFAQDSTSIDNEAAAWGDDGIRSKTVMNIQPLPRTSHDCEIIAS
                     AIVQGNSYQHYSGQYTMPSGAWFSGEPQAGTIIKFQNLPADFPSTLQAESVTQVVTTL
                     DHLTPEHFTHAISFGPIDTQSAAMSVLRSTDNVFTPQDTAQIPDVIEISSLGLTTVPD
                     VTTVSISGWDANSYYLAISDPPPNPGGYEVRYSDNGWGADDGHNLVIRTTNQTFAIPR
                     NARGKVCYIRAHDSRNALLYSEDFTQSAWIKGGGTPPTVSNAKGKDPDDKTA"
BASE COUNT          698 a          752 c          825 g          717 t
ORIGIN      
        1 gccgtcttat cgtctggatc tttgcctttg gcgttgctta ccgtgggagg cgtgccgccg
       61 ccctttatcc aggcagactg tgtaaaatcc tccgagtaga ggagagcatt acgggaatca
      121 tgggcacgga tatagcacac cttacctctc gcattgcgag gaattgcgaa ggtctggttc
      181 gtagtgcgaa taacgagatt atggccgtca tcggctcccc acccgttatc tgagtaccgt
      241 acctcatacc cgcctggatt tggcggtgga tctgagatcg ctaggtaata actgttggca
      301 tcccacccag aaatactgac ggtggtaacg tctggaaccg ttgtcaaacc aagagaggaa
      361 atctcaatga catctgggat ctgagcggta tcttgcgggg taaacacgtt gtcagtgctc
      421 cgcagaacgg acatcgccgc agactgcgta tcaatcgggc cgaagctaat ggcgtgggtg
      481 aaatgctctg gtgtcaaatg atccagggtg gtgacaacct gtgttactga ttccgcctgt
      541 aaagtggagg ggaaatcggc agggaggttc tggaacttaa taatggtccc tgcttgcggc
      601 tcacctgaaa accacgcgcc cgaaggcatt gtgtactgcc ctgaatagtg ctggtaactg
      661 ttgccctgaa cgatagcgga cgcgataatc tcgcaatcgt gggacgtgcg cggcaacggc
      721 tggatattca tgaccgtttt gctgcggata ccgtcgtccc cccatgccgc agcctcgttg
      781 tcaatactgg tagaatcttg ggcaaatccg atggccgcgc cagccgagcg gtactgtaac
      841 caaaccagat cccccggctg cggtatatac atgatttccg tctcgatggt caggtatacg
      901 acaaggttcc caggatgacc gccgctgaca tccattaaat agaacgtggt gccgttgctg
      961 acaccagtgg cactgtaact gcctgtctgc ccaccagagg ctacttttgt tccaggcgaa
     1021 acacccgcac cattgatcca cagttccatt gatgcgcttg gcgcgttcgc ccaactgacg
     1081 gtcgtgtttc cactcgtgct gccccacgga atacgaatcg gattgggaac cgcgtacaga
     1141 tcaggattga ttgccgctaa cgtgttcgct ggaatgggta cgggctggtc actgaaatat
     1201 tgtaaggaag cctgccccgg attataattg cgtgtgccaa gcagagaaga tttagtggtc
     1261 gctccctgat tgctgtctac gatagtagcg acgggcgcga ggccgtcata gctgtcgatt
     1321 tcgttcgggc ctacaggcgc gggaacccag ttcagtgatc ctctcgcagc ctgtcgcaac
     1381 tgcgccatga tcgggcaact aaccgtgaca gaagtaaagg taaggttgca gtagtcaaca
     1441 acgagcgggc aataataggc gtaagtctgc tgggaggaga gcgaaactcc cgtgttgttg
     1501 aatacccaga aattcgtgat ctgacctgta ttcgggtcta cttcactgat ataggtggaa
     1561 aaataagcgg tgtctgcatc tgcaccacct ggaatctgca ctacgttacc agtagcgtct
     1621 acgaatgacc attgccccgc ctgtcgcata gacctctgcg ttgcgaagag ggttcgcatg
     1681 atgtagcgtt tgctggtatc aataggtgcc cactggttca tgtcaacttg cccattcaca
     1741 atcgggtgca agttgaaggt atcggttgcg gttgcggtag tggggctaat ctgcgtccaa
     1801 acgttgttta cttgtcgcca tgtgtccacg ctgatcccgt acaacaggct ggacaagtca
     1861 gacgtgacgt tgcccgtaaa caatccaccg atgatgcctg aagaaaactg aacgaaatcc
     1921 cattcgccat gcgtcaagcg tagtgttcct tcgagcggaa cgaggttcaa cgattgaagt
     1981 gatacatcgt aactacgatt attgctgccg ccaaccgcat tgatcgtgcc gccaaaagga
     2041 aaaaggaccg ttccggtggg atcgtagacc gtccacttgc tgtcatcaat ggtgttgttc
     2101 gtgaacgaat cgtcaatcaa cgtagccgag tccgcaccat agacagagga aatcagcgcg
     2161 aatttggctg tggctccatc accgacaaag aactcattca tgtagttctg cggttcgata
     2221 ttgccaagta cgatggcctg gttaacgata ctatctgcta cgggctggag gcggagagaa
     2281 ccgggggtga agttgacgtt ggctccgtct accgttatgg tgaggggcgt tgaatccttg
     2341 ggcgcaaacg tcatagccct actcacacaa gagaagcgga aattggcaga ctgagcgaag
     2401 gacttcacta catccgagaa atgctggttt gggtctacga catagcgcgg caacagcatt
     2461 ccatcttgga taccagagac attaaacgaa acggtagcgg ggctaagaat cgctacgagt
     2521 tgtttgagaa tttggccttg cgtctggtta atgaagggtg gaatgatgcc aagcgggtgc
     2581 ttgttcaata caagctcgtc cgatgtcgcc tgatacttcc aagcccatcg aggccctacc
     2641 gttctctgct caccgatata cgttacttct ggatcagtga tgatgtagcc ggtgaaccaa
     2701 gccccataag tagctgtggt gatggtgacg tatgcccctg ccttcggcat tgacaccttg
     2761 cggttgtagc tgaacaggga aaaatcaagg agattggggt ccgccgcgtt gtcagtcttt
     2821 tgaaccccgc cgtagatgta acgggtgtaa tctgtgacta cacccgcctc gcttatggta
     2881 atgacattgg tgggcattac cgctcgtggt ccctcatatc cttaagaagt tccaagacac
     2941 gctcccgccc gagaacagag attagcgatt tgcgcttttc agtgtttcct tt
//
LOCUS       DATMTI010000735         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_537571, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000735 DATMTI010000000
VERSION     DATMTI010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537571"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /gene="fusA"
                     /locus_tag="VN039_11765"
     CDS             complement(<1..1892)
                     /gene="fusA"
                     /locus_tag="VN039_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWV46677.1"
                     /translation="MAHIDAGKTTTTERILFYTGITHRIGEVHEGTATMDWMEQEQER
                     GITITSAATTCTWKNIRINIIDTPGHVDFTAEVERSLRVLDGAVACFDAVAGVQPQSE
                     TVWRQADKYKVPRICFINKMDRAGADAVYATKTIVDRLGARAVPLNIAIGAEAKFAGV
                     IDLVTMKAILWHDETMGAEYSVEEIPADLLETAKEARHNLIEAVAENDDELMHLYLEG
                     QEPTEAQLKAGIRKATIAMKIFPVLCGSAFKNKGVQTLLDAVVDYLPSPLDIPAIVGH
                     NPDNMEEEVIRHADDNEPFAALGFKIMTDPFVGQLIFIRVYSGQLKTGDSVLNPRTGK
                     TERIGRLLKMHANKREEITEILAGDICAAVGLKNLVTGDTICNEKNPVALESIDFPKT
                     VIEVAVEPKTKADQEKMGMALAKLAQEDPTFNVHTDVDSGQTIIAGMGELHLEIIVDR
                     MMREYKVEANVGKPQVAYRETIRANAEAEGKYIRQTGGSGNYGHAKIRISPNEPGKGY
                     EFTNDTKGGAIPKEYIKPIDQGIQDAMLRGILAGYEMVDIKVSLYDGSYHDVDSNEMA
                     FKIAGSMAFKEAAKKAKPVLLEPMMDVEVTVPEEFMGTIIGDLNSRRGRIEGMEMVGG
                     TQAIKAT"
BASE COUNT          417 a          611 c          563 g          479 t
ORIGIN      
        1 gtcgccttga tcgcctgcgt gccgccaacc atctccatgc cttcgatgcg accacggcgg
       61 ctgttcaagt cgccgatgat cgtgcccatg aactcttcgg gaaccgttac ttcgacgtcc
      121 atcatcggtt caagcaggac cggcttggcc ttcttcgcgg cttccttgaa ggccatcgag
      181 ccggcgatct tgaatgccat ttcgttcgag tcgacgtcgt gataactgcc gtcgtagagc
      241 gagaccttga tatcgaccat ctcgtagcca gccagaatgc cgcgcaacat ggcgtcctga
      301 atgccctgat cgatcggctt gatgtactcc ttgggaattg caccaccctt ggtatcgttc
      361 gtaaactcgt agcccttgcc cggctcgttg gggctgatgc gaatcttggc gtgaccgtaa
      421 ttacccgaac caccagtctg acgaatgtac ttgccttcag cctcggcgtt ggcgcggatc
      481 gtctcgcgat aagcaacctg tggcttgccg acattggcct cgaccttgta ctcgcgcatc
      541 atgcgatcga cgatgatctc aagatgaagc tcgcccatac cggcgatgat ggtctggccc
      601 gaatcgacgt ccgtatgaac gttgaaggta gggtcttcct gcgccagctt ggcgagggcc
      661 atgcccatct tctcctggtc agccttagtc ttcggttcaa ccgcaacctc gatcacggtc
      721 ttcgggaagt caatcgactc gagcgcaaca ggattcttct cattgcagat cgtgtcgccc
      781 gtcaccaggt tcttgaggcc aaccgcagcg cagatatcgc cagcaagaat ttctgtaatt
      841 tcttcacgct tattggcgtg catcttcaac aaacggccga tgcgctccgt cttgccggta
      901 cgcggattga gaaccgagtc cccggttttg agctgacccg aatagacacg gatgaagatg
      961 agctgaccga caaacggatc ggtcataatc ttgaagccga gcgctgcgaa aggctcgttg
     1021 tcgtccgcgt ggcggataac ttcttcttcc atattgtcgg ggttgtgtcc gacaatggca
     1081 ggaatatcca gtgggctagg aagatagtcg accacggcat cgagcagcgt ctgcacgccc
     1141 ttgtttttga aagccgaacc gcagagcacc gggaagatct tcatggcaat cgtagccttg
     1201 cgaatgccag ccttgagttg cgcctcggta ggttcctgac cttcaagata gaggtgcatc
     1261 agctcgtcat cattctcggc gacggcttca atcaggttat ggcgggcctc tttggcagtc
     1321 tccagcaggt cagcggggat ctcttccacc gaatactcag cgcccatcgt ctcgtcgtgc
     1381 cagagaatcg ccttcatcgt gaccagatca atgacgccgg cgaacttagc ttcggcgcca
     1441 atggcgatat tcaaaggcac ggcacgcgca ccaaggcgat caacgatcgt cttcgtcgcg
     1501 taaacggcat cggctccggc acgatccatc ttattaatga agcagatacg tgggacctta
     1561 tacttgtcgg cctgacgcca caccgtctcc gactgcggct gaacaccggc cacagcatcg
     1621 aagcaggcaa ccgcgccgtc gagcacgcgg agcgaacgct ccacctcagc cgtaaagtcc
     1681 acgtggccgg gggtgtcgat gatgttgatg cggatgttct tccaggtgca ggtcgttgcg
     1741 gccgacgtaa tcgtgatgcc gcgctcctgc tcctgctcca tccagtccat ggttgcagtg
     1801 ccctcatgca cctcgccgat acggtgcgtg atgcccgtat agaagaggat gcgctcggtc
     1861 gtcgtcgtct tgccggcgtc gatgtgcgcc atgattccga tatttcggca acgatttaga
     1921 ggtgtagtgc gtgccacggt caatctctca tctgcgagct tccgctcgcg gtaaaaagtg
     1981 aaaaactaaa aagcttctag ctgacaggct tcttccttac caacggtagt gagcaaacgc
     2041 cttgttcgcc tcagccatgc ggtgaacgtc
//
LOCUS       DATMTI010000736         5217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_155112, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000736 DATMTI010000000
VERSION     DATMTI010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155112"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            486..1901
                     /locus_tag="VN039_11770"
     CDS             486..1901
                     /locus_tag="VN039_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWV46678.1"
                     /translation="MPNSVPPFTLVDVPGGAPLFGHLGTFKRAPLETMSGWWRQYGDA
                     LRFRLGPKTLHLFSHPDLAEEILVQQAGRFVKVYDPRRPVGLALVLGNGLVTSSGEVW
                     KRHRRIIQPVFHRSRMAAMADRMAQVGEQRIAGWTDCEGRPVDIAAEMMQLALEVISQ
                     TMFTTSMAQHIEHISRALRVSLKYAFDSFHNPLHLPTWVPTQRNREFRSVMQFMDGLI
                     YELLTERRQSGTQYGDLLDLLLQAHDEETGARLTDQELRDEALTIFAAGHETTANALA
                     WTWYLLATHPEAKARFHEEVDLVLQGRTPNADDLQRLPYTRAIFDESLRLYPPAPAVQ
                     RKVATDTTVCGLPLPAGALVLVGTHNLHRHPAFWTNPDHFLPERWLNGERPAARYAYL
                     PFGAGPRACVGIHFASVEGPLLLALIGRRYDLQLAQETVEPELMVTLRPKGGISMTIE
                     PRHIPVSLPIKQAATRASDVS"
     gene            complement(1927..2292)
                     /locus_tag="VN039_11775"
     CDS             complement(1927..2292)
                     /locus_tag="VN039_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46679.1"
                     /translation="MDFVHDTLADGRPFRILTVVDNWSRSSPVREAGFRMSGELVSQV
                     LDRVLGEGQRPRSITVDLTPSEFVTQRQEEPTAEEVVCSGYELSRNEANVRTLKVLHY
                     QCLLDGEAYNRLIVIAGGG"
     gene            complement(2439..2918)
                     /locus_tag="VN039_11780"
     CDS             complement(2439..2918)
                     /locus_tag="VN039_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWV46680.1"
                     /translation="MKRSKFSEEQIVYALRQTDSGTPIGDVCRQLGIAEQTFYAWKKK
                     YAHLGVSELRRLRQLEEENSQLKRLVADLSLDKPMLTEALRKKSEARPAPGTGPVVSR
                     AVSGEWCAGLSLGPVRSDLVVSKEPGEGSLRTPAPQSRSGACAASVRLPADLGIAPA"
     gene            complement(3485..3850)
                     /locus_tag="VN039_11785"
     CDS             complement(3485..3850)
                     /locus_tag="VN039_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46681.1"
                     /translation="MIMPATILIIEDHASVRTLLARVLEDAGYQVCEAATGRQGLERF
                     REQPVDLVITDLEMPEMNGLEVILELTRAFLDVKVIAMSGRSADELQKAKLIGARQTF
                     PKPLDLHALLHAVQYELQH"
     gene            complement(3900..4145)
                     /locus_tag="VN039_11790"
     CDS             complement(3900..4145)
                     /locus_tag="VN039_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46682.1"
                     /translation="MTCTRCQGLMLEERMIDMEAGYGEMWNCSWRCFNCGHRDDAVMR
                     HHRQLHAKPIVVSPQAVTVLETAELSWESEPVEPLAA"
     gene            complement(4308..4616)
                     /locus_tag="VN039_11795"
     CDS             complement(4308..4616)
                     /locus_tag="VN039_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46683.1"
                     /translation="MDSGQGGIDPIKIQAIRECLEKKFVDVTFNQKIRPTFTFKDGVR
                     KLSWLLVMNQEFLADRMSVEELRRFVEEKVMKKIHENPGKRIQISKYRDITVEEKNLS
                     "
BASE COUNT         1106 a         1501 c         1442 g         1168 t
ORIGIN      
        1 gatgcatttc ccaaggggcg gacacactgt actgcgaatt gtaccgtttg tggagctcct
       61 acagcatgaa tctattcacg gaagtatctt cggcggcaaa ggccgaacag ccatatctca
      121 gccgatcctc ccggaagtgc agagagacac gcagaaaatg aaactacact gtcatcaaag
      181 atgtatggtg gtgtaaaggc ggtggcacgg acagcaagga tcgtattgct cagcgaaccc
      241 ctgtcgtaga ctcttttgca gcttgtttct gatcagcgtt ttcttcggaa gacgatgcca
      301 cggtgcagat taccgaggtc actgccgatt tgtttctcct gatgcctttc atcgagtcac
      361 tcggtctcct actcagccaa ccagttcctc atgcacgagg acaagccgat gcagttacat
      421 acaagcccgc tgcagtactc cattcattga tgcccccgac ctagtgtgat acggtccgtc
      481 ctctcatgcc gaactcggta cctcccttca cgcttgtcga tgtgccaggt ggcgcgcctc
      541 tgttcggcca cctgggaacg ttcaaacgcg ctccgttgga gacgatgtca gggtggtggc
      601 gtcagtacgg cgatgcgctg cgctttcggc tcggcccaaa gactctccat ctcttcagtc
      661 atcctgatct cgccgaagaa attctggtcc aacaagccgg ccgctttgtg aaagtgtacg
      721 acccccggcg gccggtcggc ctcgcgctgg tcctgggcaa tggattggtg accagttcgg
      781 gcgaggtgtg gaagcgtcat cggcgcatca tccagccggt ctttcaccgc tctcggatgg
      841 ccgcaatggc cgatcggatg gcccaggtgg gtgagcagcg gattgccggt tggacagatt
      901 gtgaaggaag accggtcgat atcgccgccg aaatgatgca gctggccctc gaagtgattt
      961 cccaaacgat gttcaccacc agcatggcac aacatatcga acacattagc cgtgcgctgc
     1021 gcgtgagtct gaagtacgcg ttcgactcgt ttcacaaccc actgcacctc cccacttggg
     1081 taccgactca acgcaaccgt gagttccggt ctgtcatgca gttcatggat ggactgatct
     1141 atgaactgct tactgagcgg cgtcaaagcg gaacgcagta tggtgatctc ctcgatctgc
     1201 tgctccaggc tcacgacgaa gagaccggtg ccaggctgac cgatcaagag ctacgggacg
     1261 aggccttaac gatctttgcg gcgggacacg aaaccaccgc gaacgcgctt gcctggacct
     1321 ggtatcttct cgcgactcac ccagaggcga aggcacggtt tcacgaagaa gtggacttgg
     1381 ttctccaggg aagaacgcca aacgccgacg atcttcagcg cctcccctat actcgggcga
     1441 tattcgatga atcgctacgt ctgtacccgc cggcaccagc cgtacagcgc aaggtggcga
     1501 ccgacaccac agtctgcgga ttgccattgc cggctggcgc actcgtcctc gtcggcacac
     1561 acaatttgca ccgacaccca gccttttgga cgaaccccga ccactttctg ccggagcgat
     1621 ggctgaatgg cgagcggccg gctgctcgat atgcctatct cccgttcggc gcaggaccgc
     1681 gcgcctgcgt gggaatccat ttcgcgtcag tcgaagggcc actcctgctg gccctcatcg
     1741 gtcgtcgcta tgatctgcaa ctggctcaag agactgtcga acctgaactc atggtgacct
     1801 tgcggccgaa aggtggcatc agcatgacaa ttgagcctcg tcacataccg gtctccttgc
     1861 ccatcaaaca ggcagcaaca cgcgcaagcg atgtatcgta ggaaagaaaa agattactgg
     1921 aaacgcctat ccgccgccgg caattacaat aagccgattg taagcttccc cgtcaaggag
     1981 acactggtaa tggagaactt tcagcgttct gacgttggcc tcattccgag acaactcgta
     2041 accagagcag acgacttctt cggcggtcgg ttcttcctga cgttgggtaa caaattcgct
     2101 cggggtcaga tcgaccgtga tagagcgagg cctctggccc tctcccagga cacgatcaag
     2161 tacctgactc accagctctc cagacattcg aaatccggcc tctcggaccg gactcgagcg
     2221 actccagtta tcgacaaccg tcagaatgcg aaacggccgc ccgtcggcca gggtatcatg
     2281 cacgaaatcc atgctccagc gctcgggggg gggccgccgg tattggcgct ggtccacgat
     2341 gtcgcgcgat gtgtgtccgc cttcggccgc gcatgcggag cggcaaacca tcaagtcgat
     2401 acaatcgtcg aacgcgcgtg cgattgatcc gccagccctc acgccggagc aatacccaga
     2461 tccgcaggta gccgaaccga ggccgcgcat gcgccagatc gcgactgcgg agccggagtg
     2521 cggagtgatc cttcgcccgg ctctttcgat accacgaggt ccgaccgaac tgggccaagc
     2581 gacaggcccg cgcaccactc acctgaaacg gcccgtgaaa ccactgggcc agttcccggc
     2641 gccgggcggg cctcagactt ttttcgcagc gcctccgtca acatgggctt gtcgagggag
     2701 agatcggcga ccaaccgctt caactggcta ttctcctctt ccagctgtcg caatcgccgc
     2761 agctcgctca cgccgagatg cgcatatttc ttcttccagg cgtagaaggt ttgctcggca
     2821 ataccgagtt gtcggcagac atcgccgatg ggggtacctg agtcggtttg ccggagggca
     2881 tacacaatct gctcttccga gaattttgac cgcttcatcg atccgctcct ttcgcaaggg
     2941 accgccgaag ccgtcatctt actctcgttt cgaactgtcg tcgttttccg gggagacgtc
     3001 acctgttcca cgtcttctga ggcttgttta tcaaatgaac gagagaggtc tttatcgaac
     3061 tggcccagat tgtagacttc aatgagcagg aaaacttata gcgtctcgat tgggatgaga
     3121 agcaattagg cagcgaggct gtgcgaattg atgagctgtt gcgccgcatg ctcggcagcc
     3181 gctatggcct cggggagtgt cgcaaaggtg gcaatggaaa tattttaaac gggtagcgga
     3241 aacgctggaa acgctatcgg gcccagaata caaaattgtg ccgttcacca agctccgccc
     3301 ttgcaagagg ccggagcttt ctttttgctc tcgccggcgt ggtcctcgtg atgacaaggt
     3361 ggctgatcga cgggcctgcc gttctttctc ttcgataccg cgagcgtcaa agtggggcgc
     3421 gccgcattaa tcgtcagtgc cggtcgtgcg gatggtcgga acttcactga accacggtat
     3481 atggctagtg ctgcaactca tactgcaccg cgtggagcaa tgcgtggaga tcaaggggtt
     3541 tcggaaaggt ctgtcgagct ccaatgagct tggccttctg aagctcgtcg gcagagcgtc
     3601 cgctcatggc aatgaccttc acgtctaaga atgcccgggt caattccaag atgacctcta
     3661 agccgttcat ctcgggcatc tccagatccg taatgaccag atcgacaggc tgctcgcgga
     3721 accgttcgag tccctgccgg ccagtggcgg cctcacagac ctgataccca gcatcttcaa
     3781 gcacccgtgc cagcagcgtg cgcacggacg cgtgatcttc aatgatcagg atggtggctg
     3841 gcatgatcat cttgtctctt tctcacatcg gaaatcctgt acagggattg cccacctctt
     3901 caagctgcga gcggttcaac cggctccgat tcccaggaca gctcggcagt ctctagaact
     3961 gtcactgcct gagggcttac tactatcggc ttggcgtgta gctgacggtg gtgccgcatg
     4021 acagcgtcat ctcgatggcc acaattaaaa catcgccagc tgcagttcca catctcgcca
     4081 tagcccgctt ccatgtcgat catacgttct tccagcatca agccctgaca gcgtgtacaa
     4141 gtcatgatcg tcatcctttc tttggttgat cgcatcgtgt ctgagataac cgtaccaggt
     4201 aggacgatct gagccagatc caaacgaggt acgtcgaaag ggggggagag cggagaggag
     4261 tgaccactcg tcgatcttaa gcgttccgtt gttccgctgc aggaacgcta cgaaagattt
     4321 ttctcttcga cggtgatgtc ccgatacttc gaaatctgaa ttcgcttgcc agggttctca
     4381 tggatcttct tcatgacctt ctcttcgacg aatcgccgca actcctcgac ggacatgcga
     4441 tcagctaaaa actcctggtt catgacaagg agccaggaca gcttgcggac accatcctta
     4501 aaggtaaatg tcggccttat cttttgattg aacgtcacgt ctacgaattt cttttcgaga
     4561 cattcccgta tggcttgaat tttgatcgga tcaattccac cttgaccact gtccatggat
     4621 gtctccctac cttgaatggg tcacctgtta tgacgtatga gtggcacggg gataggagag
     4681 tcggtctgca tcattggagt ttacctgaaa cgctttgtgt ttcaacgaaa gcctgatttc
     4741 tagaatcgaa agaggctgga gctttcttgt tgtgtgtgcg gccgctgggt ggtgctacgg
     4801 ctggacgcgc aagcggcgcg tgtgctggaa aatgcacttc ggacaagtgt agtggataag
     4861 gagctcgcca tcgaccagcc gttttctcat gggcactgtg caccaggtgc agaggcgatc
     4921 gggtagtgcg gtcaccggtg ggagcattcc atcaggcatc gcgttcctac agacaggagg
     4981 ccgaggattg gagtgaatca acgatctgtt gcgcctgggt tagcacggcg gcgacctctt
     5041 caggggttct gaaggtgccg tcagcacgcc ctttctggta ccgccagtct ctggttccaa
     5101 gctcaaaaat gacatagtga cagctccagc ttctccatgg cgatggatgc gcatcaatcc
     5161 tctggctcgc tggtcgggtt atccataagt cttctagacc atttcctgag ggttgat
//
LOCUS       DATMTI010000737         1862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_621646, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000737 DATMTI010000000
VERSION     DATMTI010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621646"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VN039_11800"
     CDS             complement(<1..983)
                     /locus_tag="VN039_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWV46684.1"
                     /translation="MTRFERTPTPLVYGAIVLSIGVVFISDLLTTVGITVWVFYLLPL
                     VFSYLAWRPLVPAYTATATTLLILVGFFLSPPGVAPFIAAQNRAFEVTASWALAALGY
                     QFIRNKLAARKQEWLQSGQTLLSERLAGDPSVDQLGTRVLGTIAEYLDASAGAVFIEN
                     GPSFRRTATHGVPTDACIPMEVQAGDGLLGRALLEQKVFVVSDVPEGYMTIGSSLGRS
                     APRHLLIAPLAVERSIKGVLELGFFRPVGESDKEFVSRVSESIAVAVRSAEARAHIQE
                     LLEETQCQAEELQTQSEELRAANEELTEQSARLQDSHTRLEEQQAELEQSNV"
     gene            complement(1201..1665)
                     /locus_tag="VN039_11805"
     CDS             complement(1201..1665)
                     /locus_tag="VN039_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV46685.1"
                     /translation="MSTCSVSHSQSSIADRSKAKCIRVLLVDDHFLVRQGLRKYLSHH
                     IDIELVGEATDGEEAVKLTDLLNPDVVVMDINMPRMNGIEATGSIIRKHPHLPVIGLS
                     FYVGNGNREALLDAGACQLLEKGTAYQHLPHAIYQAVGKNIEARSTADGPLA"
BASE COUNT          410 a          536 c          520 g          396 t
ORIGIN      
        1 acgttgctct gttccagctc ggcctgctgt tcttccaacc gggtgtggga atcctggagc
       61 ctggcgcttt gctccgtcag ctcttcgttc gccgcgcgca attcttcgct ttgcgtctga
      121 agctcttccg cctggcactg agtttcttcc aacaattctt gaatgtgggc gcgggcctcg
      181 gccgagcgga ccgcaaccgc gatggactcg gacacccggc tcacaaattc cttgtctgac
      241 tcccccaccg gacggaaaaa tcccagttcc agcacgcctt tgatcgagcg ctctacggcc
      301 aacggggcga tgagcagatg tcgcggcgcg ctacgaccca gtgaactccc gatggtcata
      361 taaccctccg gcacatcgga cacgacgaac actttttgtt cgaggagcgc cctaccgagc
      421 aaaccgtcgc cggcttggac ttccatcggg atgcaggcat cggtcgggac accgtgcgtc
      481 gccgtccggc ggaacgacgg gccgttctcg atgaatacgg cgccggccga agcgtcgaga
      541 tattcggcaa tcgtgccgag cacccgcgtt ccgagctggt caacggatgg atcaccggcc
      601 agccgttcac tcaggagggt ctggccggat tgcaaccatt cctgtttgcg ggcagcgagc
      661 ttgttccgga tgaactgata gcccagcgcc gccaaggccc aactggccgt tacctcgaaa
      721 gcacggttct gcgccgcaat gaacggggcg acaccgggtg gactcaggaa aaagccgacg
      781 agtatcagca gcgtggtcgc cgtggctgtg taggccggaa ccaacggccg ccatgccaag
      841 tatgagaaga ccaacgggag gaggtaaaag acccagacgg taataccgac cgtggtcaag
      901 agatcgctga tgaacacaac gccgatcgac agcacgatcg cgccgtagac caacggcgtc
      961 ggggtacgct caaaacgagt catgtagggt tcgcatgatc gaagtgaaaa agaccggcca
     1021 ctccctcttc ttgtcacacg atgcgggggg ggaatgtact acgcatcggc gtcttcaggc
     1081 cctaggtaaa gcccgagtca aaacttctct ttgcataagc cacgggtcag gggaataggt
     1141 ttgtgaagtc gagagattgg agaagaactc tgcgcacgaa cttttggccc ctttccgctg
     1201 ctatgcgagg ggaccgtcag cggtcgatcg ggcctcgatg tttttgccga cagcctgata
     1261 aatcgcatga ggcaggtgtt gatacgccgt ccccttttcg agcaattggc aggcaccggc
     1321 atccaataac gcttctcgat tcccatttcc gacgtaaaac gagaggccga tgaccgggag
     1381 gtgcggatgt tttcgtatga tggagcccgt cgcttcaata ccgttcattc tcggcatgtt
     1441 gatgtccatc acaacgacgt cgggattcag caggtctgtc agctttaccg cctcttcccc
     1501 atctgtcgcc tcacccacca attcaatgtc gatatgatgg ctaaggtact tgcgcaaccc
     1561 ttgccggacg aggaagtggt cgtccaccag caacacgcga atacatttgg ccttcgaccg
     1621 gtcggctatg gaactctgag aatgggagac tgaacatgtc gacataccgc acccttgttc
     1681 gatcaacggc tggtttgccg gttaaaaccc agttaaaata actaagagat acatggtaac
     1741 agcgtagcat gatctctatt ttcgaatgac tagggagtac cctaaatccc aatggaatat
     1801 ctgcaacatg caatagatgc gcctcccggt gcatcatggt tcttacactt gtttattgtg
     1861 gt
//
LOCUS       DATMTI010000738         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_241392, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000738 DATMTI010000000
VERSION     DATMTI010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241392"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1800
                     /locus_tag="VN039_11810"
     CDS             <1..1800
                     /locus_tag="VN039_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWV46686.1"
                     /translation="DTAMVEPAPPSSSFSITLRTKLVLSTTVILIVACLLLGWLFIQQ
                     QVRSAAESLVQSGTLLAQYLAGMGRFSIVAGDTYRLEQLIHEVLAVSPVAYVAVVSSS
                     GELQAGFGKQEWQQQFSAQPVGRRQFPVTNLVQSHRPNADMNEPLVSGIWLANEGPVL
                     QSNVEFTPGELFSLMGGSELPIFYDIMVQVPHHSRGTVWDPALQLTLDERFDTQESPT
                     ALQPMAPSLVQVGLSTSNLQHVLRRLLWKAVLITLSTLTGGVCVIVLLARRITIPLQG
                     LTTAATKLAVGETVPIVAIRTHDEIGTLTQVFNTMAATLQSREHELRELAHTLEDRVD
                     ARTQELAAANTKLREIDRRKSIFISTASHELRTPLTSMKVHLANLRDGIDGAVTDDQR
                     RSLVRMEANLSRLRILIDDLLDLSQIEMGQAIVRLEPVALGNVIAKTVEDLHPFSSER
                     GVNIVISLPSDLPLVCADPDKLRQILLNLLHNAVKFTPVDTIVDLIVIRISADEIRIS
                     VRDVGPGIAQEDVDKIFQPFYRAPTAQKKTKGAGLGLTIAKLLVELHHGRLWVETEPN
                     RGSCFLFTLHSATPAQSVMAETVAPSPSHHQIA"
     gene            complement(1952..3343)
                     /locus_tag="VN039_11815"
     CDS             complement(1952..3343)
                     /locus_tag="VN039_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV46687.1"
                     /translation="MQARILIVDDDQDILSALKKRLTWMGHETLTAEDGEQALRLATE
                     ESPDLMLLDIELPGLSGLDILKRLAEKRSGFPPQTVPEVIVITAFGTIDRAVEAIRLG
                     ACDFLTKPFEPDHLSIVIEKAMAQMALTRQIGLLQAEVGGRYEHVVGQSPRMVELLET
                     ARRAAGSSATVLLLGETGTGKEVIARALHRWSPRAAKPFVVVNCAALPESLLENELFG
                     HEKGAYTGAGRREPGKIESAEGGTVFLDEIGDMPTGLQTRLLRLLQDQEFYRVGGAQP
                     IRTNVRFVAATNKDLKEAIQEGLFRQDLFYRLNVISLTLPPLRKRPEDLPALVDHFVR
                     RHGTTMGRRPFTVSQEALELIRGYHWPGNVRELENVLVRAIALCPDDCLEPEHLGITV
                     QRKLPVEADLAEDDSLNYHAVMEHYSRKVLEEGLRRAGWNQTKAAELLGLQRTYLTRL
                     IRQRGVSAKPPST"
     gene            complement(3465..>3681)
                     /locus_tag="VN039_11820"
     CDS             complement(3465..>3681)
                     /locus_tag="VN039_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWV46688.1"
                     /translation="YLNGHPNIVSRLNTLRDEGCAVSVISLAELYEGVYNSSNPAGNE
                     RASRSGQFKPSSYGRSNTLAACRGRML"
BASE COUNT          768 a         1109 c         1008 g          796 t
ORIGIN      
        1 gacacggcaa tggtcgaacc ggctccacca tcctcctcat tctccatcac gctcaggacg
       61 aagctcgtcc tcagtacgac cgtcattctg attgtcgcct gtctcttatt aggctggttg
      121 tttattcaac agcaagtgcg atcggctgct gaaagcctgg tgcaaagtgg aacgttgctc
      181 gcgcaatacc tggcgggcat gggtcgattc agcatcgtcg ccggcgatac ctatcggttg
      241 gagcaactca tccatgaagt tctcgccgtc agcccggtgg cgtatgtggc ggtcgtgtct
      301 tcgagcggtg agctgcaagc cggctttgga aagcaggagt ggcaacagca gttttccgct
      361 caaccggtgg gacggcgaca gtttccggtg acgaacttgg tccagtccca tcggcccaac
      421 gccgatatga acgagccgct cgtcagcggg atttggctcg ccaacgaggg cccggttctt
      481 caatcgaacg tcgaatttac gccgggagaa ttgttcagtc tcatgggagg gtccgagctc
      541 ccgatatttt acgacatcat ggtccaagtc ccgcatcact ctcgcgggac tgtatgggat
      601 cctgccttgc aactcacgct tgatgagcgg tttgatacgc aggaaagccc caccgctctt
      661 cagcctatgg ctccctcgct tgtgcaggtc gggctttcga cgtccaacct tcagcatgtc
      721 ttgcgtcgat tgttgtggaa ggcggtcctc attacgctga gcacattgac gggaggagtc
      781 tgtgtcatcg tcctgctggc acgccgaatc acgattccct tgcaagggct caccacggct
      841 gcgaccaaac ttgccgtcgg ggagacggtc ccgatcgtgg ccatacgcac ccacgatgaa
      901 atcggaaccc tgacacaagt cttcaatacc atggccgcga cattgcaatc acgcgaacac
      961 gagttgcgcg aattggccca caccctggaa gaccgtgtgg acgctcgtac gcaggagctt
     1021 gccgcggcca ataccaaact gcgggaaatc gaccgtcgca agtcgatctt catctccacg
     1081 gcctcacatg aattgcgcac gcctctgacc tcgatgaaag tgcacctggc gaatctgcgg
     1141 gatgggatag atggagccgt caccgacgat caacgacgtt ctctggtgcg catggaagcc
     1201 aacctttcac gccttcggat cttgatcgat gatctgctgg atctctccca aatcgagatg
     1261 ggccaagcca tcgtacgttt ggaaccggtg gcgctgggga acgtgatcgc caaaaccgtc
     1321 gaagatcttc acccgttcag ctccgaacgc ggtgtgaaca tcgtgatctc gctcccgtcc
     1381 gatctgcccc tcgtctgtgc cgaccccgac aaattgcgac agatcctcct caatctcctt
     1441 cacaatgcgg tcaaattcac gccggtggac acgatcgtcg atctgatcgt catcagaata
     1501 tccgccgatg aaatccggat ctccgtgcgt gatgtcgggc ccggcatcgc ccaggaagat
     1561 gtggacaaga ttttccagcc gttctatcgc gcgcccaccg cacagaagaa gaccaaggga
     1621 gcagggctcg gactgaccat cgccaagttg ctggtcgaac tacatcatgg gcgactgtgg
     1681 gtggagacag aacctaaccg aggcagctgt tttttgttta ccttgcactc ggccacaccg
     1741 gctcaatcgg tcatggcaga gaccgttgcg ccgagtccat cacaccatca aattgcctaa
     1801 caggatgctg aaaaagtctg ccagctgcgt tctcgcatcg ctcagaggct caacgtaccg
     1861 aagcgtacgc ctcacctctt cgctcgctgt ggccttgctg gacagccttt ttgagcatcc
     1921 tgaagttatt ctggcctgag caccagcgcg gttacgtgga gggaggcttg gcagagacac
     1981 ctctctgcct gatgagtctc gtcaaatagg ttcgttgcag cccgaggagt tccgccgcct
     2041 tggtttgatt ccatcccgca cgacgcaacc cctcttcgag aacttttcgg ctgtagtgct
     2101 ccatcacggc gtggtagttc agactatcgt cttccgcaag gtccgcttcc accggcagct
     2161 tgcgttggac cgtgatcccg agatgttccg gttcaagaca gtcatcgggg cagagtgcga
     2221 tggcccgcac caggacgttt tccaactcgc gcacgtttcc aggccagtga tacccgcgaa
     2281 tgagctccag agcctcttgg ctcactgtga aaggtcgacg gcccatggta gttccatgtc
     2341 gccggacaaa atgatccacc aatgcaggca gatcctccgg tcgtttccgc aagggcggca
     2401 acgtgagcga gatgacgttc aagcgataga acaaatcctg acggaacaat ccttcctgaa
     2461 tggcctcctt cagatccttg ttcgtcgccg cgacaaaacg cacgttggta cggataggct
     2521 gagcgccgcc gactcggtag aactcctgat cctgcaacag ccggagtaga cgggtctgaa
     2581 gtccggtcgg catgtcgccg atctcatcga gaaagacggt gccgccttcc gccgactcga
     2641 ttttccccgg ttcgcgcctg cccgctccgg tataggcccc tttctcgtgt ccgaacaact
     2701 cattttccaa taaactctcg ggtaacgccg cgcagttcac gacgacaaac ggtttcgcgg
     2761 cacgtggact ccaccgatgc aacgcgcgcg ctatcacctc tttgcccgtc cctgtttcac
     2821 ccagcaacaa caccgtggcg gacgaaccgg ctgctcgccg tgccgtctca agtagctcca
     2881 ccatacgtgg actctgccct accacgtgct cataccgtcc ccccacttcc gcttgcagga
     2941 gaccgatctg acgcgtgagg gccatctgcg ccatggcctt ttcaatcacg atggagaggt
     3001 gatcgggttc aaacggcttc gtcaggaaat cgcacgcccc gagccgtatc gcctccaccg
     3061 cccgatcgat ggtcccgaac gccgtgatga cgatcacttc cggaacagtc tgcggcggaa
     3121 agccggaacg tttttcagca agacgtttga ggatatcgag tccgctgagc cccggcagtt
     3181 cgatatcgag cagcatcaga tcaggcgatt cttcggtcgc cagtctgagc gcctgttcac
     3241 catcctcagc cgtgagcgtc tcatgtccca tccacgtcaa ccgcttcttg agcgcactga
     3301 gaatatcttg atcatcatcg acaatgagga tacgtgcctg catccgacac ccttaaggaa
     3361 gattcgtgat gggctattgt agtggaagac gggactgaag ggaaagtgcg agggcatgcc
     3421 gtagggacat atggtaactc gtatggtaga ggttcagctc gcctttagag catgcgacca
     3481 cggcaagcgg cgagggtatt actcctaccg tacgaagagg gtttgaattg gccactgcgg
     3541 gaggcgcgtt cgttacccgc tggattggag gaattataga cgccttcgta cagttccgcg
     3601 agcgagatga ccgacactgc gcagccctca tcgcgcaagg tgttcagccg gctgacgata
     3661 ttgggatggc cgttcagata a
//
LOCUS       DATMTI010000739         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_621759, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000739 DATMTI010000000
VERSION     DATMTI010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621759"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VN039_11825"
     CDS             complement(<1..407)
                     /locus_tag="VN039_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWV46689.1"
                     /translation="MNKELPIVSPDKFHDECAVFGAFGHEEAANLTYLGLYALQHRGQ
                     EASGIVAGDGDQFFIQKGMGLVADIFHKSVLEKLPGHMAIGHNRYSTTGGHDLKNVQP
                     LTVNFALGNLALAHNGNLINAQMLRHELEAYGA"
     gene            complement(429..>2359)
                     /gene="purL"
                     /locus_tag="VN039_11830"
     CDS             complement(429..>2359)
                     /gene="purL"
                     /locus_tag="VN039_11830"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HWV46690.1"
                     /translation="ATGVGGILRDIFTMGARPIALLDSLRFGELHSPKNRHLMKGVVS
                     GISGYGNCMGVPTVGGEIVFNDIYSLNPLVNVFCLGLARHDKIFRGTAAGVGNPVIYF
                     GSKTGRDGIHGATMASDSFDDQSEQKRPTVQVGDPFTEKLLLEACLELMERDLLVGIQ
                     DMGAAGLTSSSCEMASRAGNGIELDLTVVPRRESGMTPYEIMLSESQERMLMVAKAGK
                     EDECIEICRKWDLDVAVVGKVTVDGILRVLDQGKVVAEIPARALADDGPRYERPYQPP
                     AYQDMLTNLNYDAIPDVKDADVALLALLESPTIASKRWVYEQYDHMVRTNTTVRPGSD
                     AAVVRIKGTKKAVAMTVDCNSRYCLLNPYEGARLAVAEAARNLVCSGAVPIGLTDCLN
                     FGNPERPDIMWQFAMAIEGMKDACEHFQIPIVSGNVSFYNETNGLSIYPTPMLGMVGL
                     IDDTERSMTQWFKQEGDDIILLGSSREDLGGSEYLKVVHAREQGSPPYLSLNTEKALH
                     DCLLSLIRDGLLQSAHDCSEGGVAVALAESCISGPERTLGAVVRLTRGRLRKDAVLFG
                     ESQSRVVVSAKPVHRQPILDHARRFGVPVEVMGTVTGERLFMSVGSEGSAEPVIDQSV
                     AMLQDRWGFSLERALNQV"
BASE COUNT          539 a          730 c          592 g          498 t
ORIGIN      
        1 atcgccccgt aggcttcgag ttcatgtcgg agcatttggg cattgatgag attgccgttg
       61 tgagccaagg ccaaattacc aagtgcaaaa ttcacagtca acggctgcac attcttcaga
      121 tcatggccac cggtggtgga gtaacgattg tgcccgatgg ccatatggcc aggcagtttc
      181 tccagcacgg acttgtgaaa gatgtcggcg acaaggccca tgcctttctg tatgaagaat
      241 tggtccccgt ctcccgcgac aatccccgaa gcctcttgcc cacgatgctg cagcgcgtac
      301 agtcccaaat aagtcaagtt agcagcttct tcgtggccga atgcgccgaa gacggcgcac
      361 tcgtcatgaa acttatcggg agagacgatt ggtagttctt tgttcatgac ccttagcaat
      421 tcacaagact agacttgatt cagcgctcgc tccaaagaaa acccccatcg atcctgcagc
      481 atcgccactg attgatcgat cacgggttcc gccgaaccct cacttcccac ggacatgaac
      541 aaccgttcgc cggtaacggt ccccatcact tcaaccggga cgccgaaacg cctcgcgtga
      601 tcgagaatgg gctgtcgatg caccggcttt gcagaaacca ccaccctcga ctgactttcg
      661 ccgaagagaa cggcgtcttt tcgaagccgg cctctggtca atcttaccac agcacccagg
      721 gtccgttcgg gtccggagat acaactttcc gccaatgcaa cggcgacacc accttccgag
      781 caatcatgcg cggactgcag aagcccatca cgaatcagcg acagcaggca atcgtgcaga
      841 gccttttccg tattcaggct caaatagggc ggtgatccct gttcgcgagc atgaacgacc
      901 ttgagatact ccgatccgcc caaatcctcg cgagaggagc ccagcaagat aatgtcgtcg
      961 ccttcctgct taaaccactg agtcatcgac cgttcagtgt catcgatcag cccgaccatg
     1021 ccgagcatgg gagtcggata aatggagagt ccattggttt cgttgtaaaa gctgacgttc
     1081 ccgctcacga tggggatttg aaaatgctcg caggcatctt tcatcccttc gatcgccatg
     1141 gcaaactgcc acatgatatc gggtcgctca ggattgccga agttaagaca atccgtcagg
     1201 ccgatcggta cagcgccgga acagacgaga ttccgcgccg cctcggccac ggccagcctg
     1261 gccccttcat agggattcaa caggcaataa cggctgttgc aatccaccgt catcgccaca
     1321 gccttcttcg tgcctttgat gcgcaccacc gccgcatcgg accccgggcg taccgtggtg
     1381 ttcgtccgca ccatatgatc gtactgttcg tacacccacc gcttgctggc gatcgtcggc
     1441 gactccaaca aggccagtaa cgccacatcc gcatctttca catcgggaat ggcgtcgtag
     1501 ttcaggtttg tcagcatatc ttgataagcc ggtggctggt agggccgttc atagcgtgga
     1561 ccatcgtcag ccaaggccct tgccggaatt tctgcgacaa ccttcccctg atccaggaca
     1621 cgcaagatac catccaccgt cactttcccg accacggcga catccagatc ccacttccga
     1681 caaatctcga tgcattcgtc ttccttgccg gctttcgcca ccatcaacat acgctcctga
     1741 gattcagaca acatgatctc atagggcgtc ataccagatt cgcgccgtgg taccaccgtg
     1801 agatccaact cgatcccgtt gccggcgcgg gaggccattt cgcaggaaga actcgtcagg
     1861 cctgcagcac ccatatcttg aatcccgacg agcagatcac gttccatcag ttcgaggcaa
     1921 gcttccagca gcaatttttc cgtaaaggga tcaccaacct ggacggtcgg ccgcttctgc
     1981 tccgattgat cgtcgaacga atcggacgcc atcgtcgccc cgtgaatccc atcacggcct
     2041 gttttggagc cgaaatagat tacaggattc ccgacgccgg cagccgtgcc acggaatatc
     2101 ttgtcgtggc gggcaagacc gagacaaaac acattgacca gcggattgag ggagtagatg
     2161 tcgttgaaga cgatctcgcc tcccacagtc gggactccca tgcagttgcc atagccggat
     2221 atcccggaga cgaccccttt catgagatgg cgattcttgg gcgagtgcaa ttctccaaat
     2281 cgcagcgaat cgagcagcgc gatcggtctt gcccccatcg taaagatgtc gcgcaaaatt
     2341 ccgccgactc ccgtggcgg
//
LOCUS       DATMTI010000740         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_835438, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000740 DATMTI010000000
VERSION     DATMTI010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835438"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1199)
                     /locus_tag="VN039_11835"
     CDS             complement(78..1199)
                     /locus_tag="VN039_11835"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWV46691.1"
                     /translation="MRTLLVIAWLLLSVPAWAHKPSDSYLSLSVHNDRVEGQWDIALR
                     DLDNAIGLDRDGNGRLTWGEVRETHGEIGAYALSRLVLSAGTQTCTTQLLDQLIDHHT
                     DGAYSVLRFRSDCGGPIEGLRVDYQLLFDTDAQHKGLLRLTHGGQTRATIFSKESPAQ
                     EFSIAKRSLWAESTQFIHEGIWHIWLGFDHVLFLLALLLPTVLIRAEGRWQAAGDFSS
                     VCWDVLSIITAFTVAHSLTLSLAALDIVQLPSRLVESTIAASVVLAGLGNLYPMMISR
                     RWMIAFGFGLIHGFGFAAVLTDLGLPHDSLLLSLVSFNVGVELGQLAIVAAVLPLAYL
                     IRRSWSYPRLVLTGGSLAVIAIALVWFTERAFDLRLFPL"
     gene            complement(1199..2386)
                     /locus_tag="VN039_11840"
     CDS             complement(1199..2386)
                     /locus_tag="VN039_11840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV46692.1"
                     /translation="MIRLLTVVRVIGWLILSQVDIMYAEPYRPTDDAQVLERLSYKAT
                     DPAAHEIERLRADLRNNPRHLESAVKLATRYIEQGRSEGDPRFLGQAQAILASWWNKP
                     TPPVSALLLRATIRQNAHEFDEALADLDQVLDKQPTNAQAWLTKASILQVQARYDEAR
                     RACQRLARLAASHVLLGCLSDIAGVTGQSVKSRELLRQVLSESAISGRERIWVATILA
                     ETAARTGAIREAEQSFSEAFKVGIKDQYLVSAYADYLLDQRRYQDAISLLQSETRADG
                     LLLRLALAEQALELPASQNHKAELAARFAASRERGTTVHVREEARFTLALLHDAGRAL
                     PLAQANWNVQREPADARILLECALADKNRAAAQPVLDWLNRNHVEDFRLRQLARQIQE
                     APF"
     gene            complement(2390..3259)
                     /locus_tag="VN039_11845"
     CDS             complement(2390..3259)
                     /locus_tag="VN039_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46693.1"
                     /translation="MNQRTTRQETRIPLLHGWLSSIALHALMLLSIFPIFRQSLIPVY
                     KEPFHWDVALVQTIPTAHEPIQTADVPHPAIAKSPDRTHTASHANRTIHHVTPSAEHI
                     TSLAPKPHAPVISSSEAATTSIPTELPANPAQATEPPLQPIETPVASTTPALPLKTAA
                     ASATLEENTPPTDSTQPSPAPATVSDTAALSAIRPDYGWLQQAISRRLEELKRSSRPS
                     LDESRPLKVTVKAVVSKEGILLDSAVVKSSGLDRIDQEAIALVQSAFPMQFDRTLDRQ
                     QIAMRIPITYSRE"
     gene            complement(3316..>4116)
                     /locus_tag="VN039_11850"
     CDS             complement(3316..>4116)
                     /locus_tag="VN039_11850"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HWV46694.1"
                     /translation="IVGAPGAGTADTFSGFNVNTIALEIPIGELIGPNGNKVLGAYAS
                     TSRPKVSVIKKSGDRNNSTRFVQVARMGNPLVNELVIPTNMKDKWNATDAEDENDFVS
                     FYCNSALATALNTVFGTGFPTANREDLVNALLRYAPGPPVCGSGKTNEALADFLRVDL
                     DVPATPAAGQKRLGPLAHDASGNATPDRAGWPNGRRPNDDVTDVALRVVAGILTNPAV
                     PNLGDGVNFNVGSVGGNKTAHGIATAFPFLPTPFDGRDRRHIDPKEAF"
BASE COUNT          829 a         1095 c         1299 g          893 t
ORIGIN      
        1 tatcttgccg ccgccgtcat gtggcttaac tgacgacacg ccctaggaat gtttcctgtt
       61 ggttttccag gattctgcta aagcgggaat agtcgtagat caaaggctcg ttcggtgaac
      121 cagacgagcg cgatggcgat cacagccagc gatccgccgg taagcaccaa tctgggataa
      181 gaccaggagc ggcggatcag ataggccaac ggtaggactg cggcgacgat ggcaagctgg
      241 ccgagttcca ctcccacgtt gaagctgacc aagctcagta acaatgagtc atgaggcagc
      301 cccagatccg tgagtacggc ggcaaatcca aacccatgga taagcccaaa tccaaaggca
      361 atcatccaac gccgacttat catcatcggg tacaaattgc caagccctgc gagcaccacc
      421 gaagcggcga tggtcgattc caccaatcgg gatgggagct gaacgatatc cagggcggct
      481 aaactcaaag ttagcgaatg agctaccgta aaagcggtga ttatgctgag gacgtcccag
      541 cacaccgatg aaaaatcacc cgccgcttgc cagcgacctt ctgcccggat caagacagta
      601 ggaagcaaga gggcaagcaa gaacaggaca tggtcgaacc ccaaccagat gtgccagatg
      661 ccctcatgaa taaattgcgt gctctcagcc cagagtgatc gcttggcaat cgaaaattcc
      721 tgggccgggg attctttgct gaaaatggtg gcacgggtct gtccgccatg cgtgagacga
      781 agcagcccct tatgctgtgc atcggtatcg aacaataact gatagtccac acggagccct
      841 tcgatggggc cgccgcagtc tgaacggaaa cgcagaactg agtaggctcc gtccgtgtgg
      901 tggtcgatga gttggtccag cagctgagtc gtacaggttt gtgtgccggc cgacagcacg
      961 aggcgagata atgcataggc gccgatctca ccatgtgtct cacggacttc tccccaagtg
     1021 agtcggccgt tgccatcgcg atccaatccg atcgcattgt cgagatcgcg taaggcgatg
     1081 tcccactggc cctcgacgcg atcattgtga acagacagcg agagatagct gtcgctgggc
     1141 ttgtgagccc aagcgggaac gctgagcaga agccaggcga tgacgagcag ggtgcgcatt
     1201 agaatggtgc ctcttgaatc tgtctcgcaa gttgtcgcag tcgaaagtct tcaacatggt
     1261 tgcggttcaa ccaatcgagt accggctggg ccgccgctcg gttcttgtcg gcgagcgcac
     1321 attctagaag gatcctggcg tctgctggtt cgcgctggac gttccaattg gcctgggcta
     1381 acggcagcgc ccgtccggca tcgtgcaaca aggccaacgt aaaccttgct tcttctctca
     1441 catggacggt cgttcctcgc tcgcggcttg cggcaaagcg tgctgccagt tcggctttgt
     1501 ggttctggga tgcgggcagc tccagtgcct gttcagcgag ggccaggcgc aagagcagtc
     1561 catcggctct ggtctcagat tggaggaggg agatggcgtc ttgataacgg cgttgatcca
     1621 ggaggtagtc cgcgtaggcg ctcaccaagt attgatcctt gatgccgact ttgaaggctt
     1681 cagagaaaga ttgttcagct tctcgtattg ctccggtgcg cgcggctgtt tcagctagga
     1741 tggtcgcaac ccagattcgc tcgcgccctg agatggccga ctccgatagg acctgccgga
     1801 gcagctctcg gcttttcacg gattgaccag tcacgccggc gatatcgctt aagcatccca
     1861 gcagaacatg agaggccgcc aatcgagcaa gccgctgaca ggcccgccgt gcctcgtcat
     1921 agcgtgcctg tacttggaga atggatgctt tggtgagcca agcctgggca ttggtcggtt
     1981 gtttgtccag cacctggtcc agatcggcca gagcctcatc gaattcatgg gcattctgtc
     2041 tgatggttgc gcgcaacaga agtgcggaaa ccggaggagt cggtttgttc caccaagagg
     2101 cgaggatcgc ctgtgcttgc ccaaggaaac gaggatcgcc ttccgatcgg ccttgttcga
     2161 tatagcgagt cgccagtttc acggctgact cgagatgacg aggattgttt cgcaggtctg
     2221 cccgtaggcg ttcgatttcg tgcgctgcgg gatcagtcgc cttgtaactg agccgctcaa
     2281 ggacctgggc atcatctgtc gggcgatagg gttcggcgta catgatgtcg acttggctca
     2341 agatgagcca tccgatcacg cgtacaactg tgagcaaccg aatcatggac tactcgcgcg
     2401 aataggtgat gggaatgcgc atagcgattt gttgccgatc caatgtacgg tcgaactgca
     2461 tgggaaaggc actttgcacg agcgctatgg cttcttgatc gatgcgatcc agaccggaac
     2521 ttttgaccac tgcggagtcc agcaagatcc cttccttcga gactactgcc ttgaccgtga
     2581 ccttgagggg gcgcgattca tcgagtgatg ggcgagaaga ccgcttgagt tcctccagcc
     2641 ggcgggaaat tgcctgctga agccagccgt agtcggggcg tatcgcggaa agagcggcgg
     2701 tgtctgatac tgtggccggt gccggcgacg gctgggtgct gtcggtaggt ggtgtgttct
     2761 cttctagggt agcggaggca gcggctgttt tcagtggcag tgccggtgtc gttgaggcca
     2821 ccggtgtctc aatcggttgt aatggaggtt cggttgcctg ggccggatta gccggcaatt
     2881 cagtcggtat tgaggttgtg gctgcctcgc tcgatgatat gaccggtgcg tggggctttg
     2941 gagcaagtga ggtgatgtgt tcagctgatg gagtgacgtg atggatcgtg cggttggcat
     3001 gagaggcggt gtgtgtgcga tccggtgact tcgctatggc cgggtgaggg acatcggcgg
     3061 tttgaatcgg ctcgtgagcc gttggtatcg tttgcacgag cgcaacgtcc cagtgaaacg
     3121 gttctttgta aacaggtatc agcgactgtc ggaatatcgg gaatatgctg agaagcatga
     3181 gcgcgtgcag ggcgattgag ctgagccacc cgtgcaggag cgggattcgc gtctcttgcc
     3241 tggtcgtccg ctgattcatg ccctggtcct ataaggcggg tgccgcatgc gaatgcggca
     3301 cccgcttggc gatgactaga aggcctcttt tggatcgata tgtcggcggt cacgtccgtc
     3361 gaacggcgtc gggaggaagg gaaacgccgt tgcgataccg tgggccgtct tgttgccacc
     3421 gactgatccg acgttgaagt tcacgccgtc gccgaggttc ggcacagcgg ggttcgtcag
     3481 aattccggcc acgacgcgca aggccacatc agtgacgtcg tcgttcggtc tcctgccgtt
     3541 tggccagccg gctctatccg gcgtcgcatt gccgctcgcg tcatgggcca gtgggcccag
     3601 tcgcttctgg ccggcagccg gagtggcggg gacatcgaga tcgacacgca ggaaatcggc
     3661 gagcgcttca ttggtcttgc cggagccgca gaccggtggc cccggtgcat accggagcaa
     3721 ggcgttcaca agatcttcgc gattcgccgt cgggaaaccg gtaccgaaga ccgtattgag
     3781 tgctgttgcg agcgccgagt tgcaatagaa tgagacaaaa tcattctcgt cttccgcatc
     3841 cgtggcattc cacttgtcct tcatgttcgt cgggatcacc agctcgttca cgagcggatt
     3901 acccatgcgt gccacctgga cgaagcgagt gctgttgtta cggtcgccgc ttttcttgat
     3961 gacgctgacc ttaggccggc tcgtgctggc ataggcgccg agcactttgt tgccgttcgg
     4021 gccgatgagc tcgccgatgg ggatttctaa tgcgatggtg ttgacgttga aaccgctgaa
     4081 ggtatcggcg gtgccggcgc ccggtgcacc gacgat
//
LOCUS       DATMTI010000741         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_368117, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000741 DATMTI010000000
VERSION     DATMTI010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368117"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..2883)
                     /locus_tag="VN039_11855"
     CDS             complement(328..2883)
                     /locus_tag="VN039_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWV46695.1"
                     /translation="MEQYQALLEVAETISVHRDLHELFRDLVQRLPRVVHVNFASLSL
                     HDPTRNHIRLQTIQANVPAELLGGHEEPLDETPAGLVLLTQQPVFVPNLADEHRWPKV
                     VQRMKEDGINSFCVVPLTTAVRRLGAMGFSSMRKEAYSELDVEFLRQVGKQVAVAVDN
                     VLHHQELIHDRDRLRLLLEVTESIAFHHDVDRLLHDLAQRLPRIVPFDYINIVLHDPA
                     KEVMRLRLLVTSIPATITPGLELPMEESPGGLVWKTQQPLTVPDIAHERRFSKLIAML
                     RENGVQSFCVVPLTTANQRLGALGFGSLERRTYEASEIAFMHQVAKQVAIAVENALNY
                     GRAQSTQSQLTHERDHQRLLLEVNNAVITHLDLDDLFTAVSECLRKVIQHDGSSLLLC
                     DEETGQWRIHVLDFQRNESFVEEGTIEESTESPSCLAINTGKAALFREQDLKEMAGSS
                     PCAQDLLDRGVKSFCSLPLLAHKRTLGALNVGRRRDDGFTSEDVELLSQVAQQVAIAV
                     ENALAYKQIAQLKDKLTEEKLYLEEEIQTDYNFEEIVGESRALKQVLKQVQTVASTDS
                     TVLILGETGCGKELVARALHNLSTRRERTFVKLNCAAIPTGLLESELFGHEKGAFTGA
                     IATKVGRFELADRGTLFLDEVGEIPLELQVKLLRVLQEQEFERLGGTRTIRTSVRVVA
                     ATNRDLVQMVEEQKFRSDLYYRLKVFPVTVPPLRERPDDIPLLVRHFAQKFAQRMKKR
                     VETIPSEAMKALQNYSWPGNVRELENFVERAVILTHGSDLYVSLTELKPTPGHVENSA
                     AITLEQAEREHILRTLRESSWIIGGTAGAAAKLGMKRTTLQSKMQKLGIARPR"
     gene            3025..3183
                     /locus_tag="VN039_11860"
     CDS             3025..3183
                     /locus_tag="VN039_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46696.1"
                     /translation="MKGDPAEVLESYDGVNLDGEAINGEVASENRETSARIVIMDIAM
                     PRHDLAHR"
BASE COUNT          722 a          992 c         1020 g          790 t
ORIGIN      
        1 attcatgggg ctctcctttt tgtcctggac atagcactct ctaaagtcgc gatcacattg
       61 ggaatgcaca tatttaaaat agtcgaggat tggatgagtc ttccttccgt tgtcacttca
      121 agatcgccca attgcgttgc gagtgattta cgatgttctt aagccatggc aaggatcgtt
      181 ccatagaaca tgcatcaagg tcgcgtactt ctaagtgcgg aacaaatatg tgagtcggga
      241 tgaattagag ggaagaagtt ggaaggaatg agccgcttgt gacgaaagat acctgtcgat
      301 atctcggcgt ttgccgaaag gtcagcatca gcgcgggcgg gcaatcccga gcttttgcat
      361 cttggattgg agggtggtcc gtttcatgcc gagcttggcc gctgcacccg cggtcccacc
      421 gatgatccag ctcgactcac gcagggtcct gagaatatgc tcacgctcgg cctgttcgag
      481 ggtgatcgcc gcggaattct ccacgtggcc cggcgtcggt ttcaattcag tcagcgacac
      541 gtagagatcc gatccatgcg tcaggatgac ggcgcgctcg acgaagtttt ccagttcgcg
      601 tacgttgccc ggccaggaat aattctgcaa cgccttcatg gcctcggatg gaatcgtctc
      661 gacgcgtttc ttcatccgtt gggcgaattt ctgcgcgaag tgccgaacca acaacggaat
      721 gtcatccgga cgttctcgaa gcggcgggac ggtgaccggg aagactttga gccggtagta
      781 gagatcactg cgaaatttct gctcttccac catttgaacg aggtcgcgat tcgtggccgc
      841 gacgacccgc acactcgtgc gaatggtacg agtgccaccc agccgttcga actcctgctc
      901 ctgaagtacc cggaggagct tgacctggag ttccagggga atttctccca cttcgtcgag
      961 gaacagcgtt ccccggtcgg ccaattcaaa ccggccgacc ttggtcgcaa tcgcaccggt
     1021 aaaggctccc ttttcgtgac cgaataattc gctttcgagc aggccggtcg ggatcgcggc
     1081 gcaattcagc ttcacgaacg tgcgttcccg ccgggtgctc agattgtgaa gcgcgcgggc
     1141 gacgagctct ttcccgcagc ctgtttcacc caggatcaag acggtggagt ccgtggaggc
     1201 gacggtttgg acctgcttga ggacctgctt gagggcacgg ctctcgccga caatttcttc
     1261 aaagttgtag tccgtctgaa tttcttcttc aaggtagagt ttttcttcgg ttaacttgtc
     1321 cttcaattgg gcaatttgct tataggccaa ggcgttctcg accgcgatgg cgacttgctg
     1381 tgcgacctga ctgagcaact cgacgtcttc cgacgtaaaa ccgtcatctc ttcgtcgtcc
     1441 cacgttcaat gcgccgagcg tgcgtttgtg cgcaaggagc ggcagagagc agaacgactt
     1501 cactccgcga tcaagaagat cctgcgcgca aggtgaagag cccgccatct ctttcagatc
     1561 ctgttctcta aacagcgcgg ctttgcccgt gttgatcgcg aggcacgacg gggattccgt
     1621 actctcttca atggttcctt cctcgacgaa actttcattc ctctgaaaat ccaacacatg
     1681 tatacgccac tggccggtct cttcgtcgca aagcagcagg ctggacccgt cgtgctgaat
     1741 cacttttctc aagcactcac tgactgccgt gaacaagtcg tcgagatcca gatgggtgat
     1801 gacggcatta ttcacttcga gcagcaaccg ctgatggtca cgctcatgcg tgagttggga
     1861 ctgagtggac tgcgcgcgtc catagttcag cgcgttctca acggcgatgg ccacctgctt
     1921 cgcgacttgg tgcatgaatg caatctcgga tgcttcgtag gttcggcgct ccaggctgcc
     1981 gaacccgaga gcgccgaggc gttggtttgc ggtggtcagc ggcacgacgc agaaagactg
     2041 cacaccgttc tcccgcagca tcgcgatcaa tttggagaag cgacgttcgt gcgcaatgtc
     2101 gggcacggtc aagggttgct gcgttttcca gacgagaccg ccgggggatt cctccatcgg
     2161 caattcaagc cccggcgtga tcgtagccgg tatcgacgta accaacaacc ggaggcgcat
     2221 gacctccttg gccggatcgt gcagcacgat gttgatgtag tcgaaaggca cgatgcgggg
     2281 aagtcgctgc gcgagatcgt gcagcagccg gtccacatca tggtgaaacg cgatggattc
     2341 cgtgacctcc agcagaaggc gtagccgatc ccgatcgtgg atcaactcct gatggtgcag
     2401 gacattatcg accgccaccg cgacctgctt cccgacctgc cggagaaatt ccacgtccaa
     2461 ctcgctgtac gcctctttcc gcatgctcga aaatcccatg gctcccagtc gtcgtactgc
     2521 ggtggtcagg ggcacgacac aaaacgagtt gatgccgtcc tccttcatgc gttgaacgac
     2581 ctttggccag cgatgttcgt cggcaaggtt cggcacgaag accggctgct gtgtgaggag
     2641 aaccaaaccg gcgggagtct catcgagagg ctcctcatgg ccgcctaaga gctcagccgg
     2701 cacgttcgct tgaatggtct ggaggcggat atggttgcgc gtcgggtcgt gcaatgagag
     2761 agaggcgaag ttcacgtgca ccacgcgggg gagacgctgg actaaatcgc gaaagagttc
     2821 gtgcaggtcg cggtgcacag agatcgtctc cgcaacttcc agtagtgcct ggtattgctc
     2881 catcagcgtg tcgcacggtg atgcggatgc cggatccata attgtagggt agtatgacgt
     2941 atccgattgt cgcggctcaa taggggggcc atttcaccgg cctccccgat gcctctatcc
     3001 gatggatact gttggtcgag gaccatgaaa ggtgatccgg ccgaggtgct ggaaagttat
     3061 gacggtgtga acctcgatgg ggaagcgata aatggtgagg ttgcgagtga gaatcgagaa
     3121 acttcagccc gtattgtgat catggatatt gccatgccga ggcacgactt ggctcaccgg
     3181 tgacttcggc agagcaagtg gaataccgcg aggcagagag aacctttcct ctctgcctca
     3241 gtgagcgggc gatcgtgtgg gcacatatag cctaataacc gcccatgcct ccgccggatg
     3301 accccatgcc ttggggtacc cccatactac cggtactacc gggcatacta ccgggtcctc
     3361 ctatgctgcc gcctgcccct aggccgggtc tctctagcga cccgagtggc gccgtgctac
     3421 ccgatgtacc catgttaccg aacgggtcat agctaccgga tgaggccctt ccgctggtca
     3481 tccccgtttg aacttcgcca agcatccgag ccggcccgcc tctt
//
LOCUS       DATMTI010000742         5748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_69834, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000742 DATMTI010000000
VERSION     DATMTI010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69834"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2881)
                     /locus_tag="VN039_11865"
     rRNA            complement(<1..2881)
                     /locus_tag="VN039_11865"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3197..3272)
                     /locus_tag="VN039_11870"
     tRNA            complement(3197..3272)
                     /locus_tag="VN039_11870"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3237..3239),aa:Ala,seq:tgc)
     gene            complement(3302..3378)
                     /locus_tag="VN039_11875"
     tRNA            complement(3302..3378)
                     /locus_tag="VN039_11875"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3342..3344),aa:Ile,seq:gat)
     gene            complement(3830..5341)
                     /locus_tag="VN039_11880"
     rRNA            complement(3830..5341)
                     /locus_tag="VN039_11880"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1154 a         1845 c         1370 g         1379 t
ORIGIN      
        1 atcaagcctt acgggcaatt agtactggtt agcttaacgc attactgcgc ttccacaccc
       61 agcctatcaa cgtcctggtc ttgaacgacc cttcaaggag ctcgaggctc cagggaatcc
      121 tcatcttcag gcgagtttcc cgcttagatg ctttcagcgg ttatctcttc cgtacatagc
      181 taccctgcga tgcctctggc gagacaacag gtacaccagc ggtacgtcca ctccggtcct
      241 ctcgtactag gagcagcccc cgtcaagatt ccaacgccca cggcagatag ggaccaaact
      301 gtctcacgac gttttaaacc cagctcacgt acctctttaa atggcgaaca gccataccct
      361 tgggaccggc tacagcccca ggatgagatg agccgacatc gaggtgccaa acaccgccgt
      421 cgatatgaac tcttgggcgg tatcagcctg ttatccccgg cgtacctttt gtccgttgag
      481 cgatggccct tccattcaga accaccggat cactaagtcc tgctttcgca cctgctcgcc
      541 ttgtcaggct cgcagtcaag ctcgcttgtg cctttgcacg tctggcgcga tgtccgaccg
      601 cgctaagcga accttcgagc tcctccgtta ctctttggga ggagaccgcc ccagtcaaac
      661 tgcctaccat gcacggtccc cgctccagat catggagcaa ggttagagcc gcaatgacgc
      721 caggctggta tttcaaggtt ggctccagga gagctagcgc ccccccttca tcgcctccca
      781 gctatcctac acaagcctca tcacagtcca atgcaaagct gcagtaaagg tgcacggggt
      841 ctttccgtct tgccgcgggg agattgcatc ttcacaaaca cttcaacttc gctgagtctc
      901 aggaggagac agcgtggcca tcgttacgcc attcgtgcag gtcggaactt acccgacaag
      961 gaatttcgct accttaggac cgttatagtt acggccgccg tttaccgggg cttcgatcaa
     1021 gagcttgcac cccatcactt aaccttccgg caccgggcag gcgtcacacc ctatacgtcg
     1081 actttcgtct tagcagagtg ctgtgttttt aataaacagt cgcagccacc gattctctgc
     1141 ggccccatcg agctcaccgc gcaagcggat caccctaccg gggcatacct tctcccgaag
     1201 ttacggtatc aatttgccga gttccttctc ccgagttctc tcaagcgcct tggaatattc
     1261 atcccgtcca cctgtgtcgg tttgcggtac ggtctcgtac agctgaagct tagaggcttt
     1321 tcctggaacc acttcctatc acttcggccc acttggggcc tcgcgccaca cccttgagtt
     1381 acgcgcccgg atttacctaa gcgccctctt caatgcagca acagggactt ccaacaccct
     1441 gatgatgatc cgcgatccgt ccccccatcg cactgtacga cggtgctgga atattaacca
     1501 gcttcccatc agctacgcat ctctgcctcg ccttaggggc cgactcaccc tgcgccgatg
     1561 aacgttgcgc aggaaacctt ggacttacgg cgagggggct tttcaccccc tttatcgcta
     1621 ctcatgtcag cattcgcact tctgatacct ccagcagcct ttacaagcca ccttcgcagg
     1681 cttacagaac gctctcctac cgcgcacacc taagtgtgca cccgcagctt cggtttatcg
     1741 cttagccccg ttacatcttc cgcgcaggac gactcgatca gtgagctatt acgctttctt
     1801 taaaggatgg ctgcttctaa gccaacctcc tgactgtctg tgccttccca cttcgtttcc
     1861 cacttagcga taattcggga ccttagctgg cggtctgggt tgtttccctc ttgagtccgg
     1921 acgttagcac ccggtgctct gtctcccgcg ctctacttgt cggtattcgg agtttgccat
     1981 ggtttggtaa gtcgccatga ccccctagcc ataacagtgc tctacccccg acagtcatac
     2041 gcgaggcact acctaaatag ttttcggaga gaaccagcta tttccagatt tgtttagcct
     2101 ttcaccccta tccacagctc atcccctagt ttttcaacac tagtgggttc ggtcctccag
     2161 cacgtgttac cgtgccttca acctggccat gggtagatca tctggtttcg ggtctacacc
     2221 cagcgactag acgccctatt cggactcgct ttcgctacgc cttccctatt cggttaagct
     2281 cgccactgaa tgtaagtcgc tgacccatta tacaaaaggt acgccgtcac cccttaaaga
     2341 ggctccgact gtttgtatgc atacggtttc aggatctatt tcactcccct tccggggttc
     2401 ttttcgcctt tccctcacgg tactggttca ctatcggtcg accacgagta tttagccttg
     2461 gaggatggtc cccccatctt cagacaggat ttcacgtgtc ccgccctact tgtcttgcgc
     2521 ctagttccac caccgccatt tcgcctacag ggctatcacc tgctacggcc gggctttcca
     2581 tcccgttcga ctatgacaga agctaaaacg caaaggctct tgcaatttcg ctcgccgcta
     2641 ctttcgcaat ctcggttgat ttcttttcct cgagctactg agatgtttca gttcacccgg
     2701 ttcgcttcac gcagcctatg tattcagcta cggatactgc ctcgcggcag tgggtttccc
     2761 cattcggaca tctgcggatc aaagcttgtt tgccagctcc ccgcagctta tcgcaggcta
     2821 ctacgtcctt catcgcctgt ggtcgccaag gcatccacca tatgcactta gtcgcttgat
     2881 cctataacgc ttacctcttt caaggtgctc tttataggtg ctgttcattc gcgtttgtgc
     2941 cgttccaaaa actgtagctt cgcgcatcct tgcggattcg ctactcccac agcttgtctt
     3001 ttgagaacta aatctatgca atcacaaccc gtatcccctt tcgcaatatt ccctcgcgga
     3061 aactcgctac tcggaaacac tttacgttgt gcttcttcct cattgttaaa gaacgatgtg
     3121 actcttgaac cccactcatc gctgagcaaa atccaatcat caaggctcgc gtaaaacctt
     3181 catgattgaa ctttcttggt ggaggatgac gggatcgaac cgacgacatc ctgcttgcaa
     3241 agcaggcgct ctcccagctg agctaatccc ccgcaatctt gccgcaggca ccttgatcgc
     3301 ttggtgggtc tggttggatt cgaaccaacg acccccgcct tatcaagacg gtgctctaac
     3361 cgactgagct acagaccccg ctctcgatct atcagtcaca cataaacaac cgataagcgt
     3421 ggacacttca gcgcttcgag cttcttactc ttaaaggagg tgatccagcc gcaccttccg
     3481 atacggctac cttgttacga cttcacccca gtcatgaatc ctaccgtggt aatcgccccc
     3541 cttgcggtta ggctaactac ttctggtaaa acccactccc atggtgtgac gggcggtgtg
     3601 tacaagaccc gggaacgtat tcaccgcgac atgctgatcc gcgattacta gcgattccga
     3661 cttcacgcag tcgagttgca gactgcgatc cggactacga tcgggtttct gagattggct
     3721 ccacctcgcg gcttggcaac cctctgtatg caccattgta tgacgtgcga agccctaccc
     3781 ataagggcca tgaggacatg acgtcatccc caccttcctc cgatttgtca ccggcagtct
     3841 ctctagagtg ccctcgactt caccccagtc atgaaccctg ccgtggtaat cgccctcctt
     3901 gcggttaggc taactacttc tggcaaaacc cactcccatg gtgtgacggg cggtgtgtac
     3961 aagacccggg aacgtattca ccgcggcatg ctgatccgcg attactagcg attccagctt
     4021 cacgtagtcg agttgcagac tacgatccgg actacgatgc gttttctgag attagctccc
     4081 cctcgcgggt tggcaaccct ctgtacgcac cattgtatga cgtgtgaagc cctacccata
     4141 agggccatga ggacttgacg tcatccccac cttcctccgg tttgtcaccg gcagtctctc
     4201 tagagtgccc ttgcgtagca actagagaca agggttgcgc tcgttgcggg acttaaccca
     4261 acatctcacg acacgagctg acgacagcca tgcagcacct gtgtccactt tccctttcgg
     4321 gcacctgatg catctctgct tcgttagtgg catgtcaagg gtaggtaagg tttttcgcgt
     4381 tgcatcgaat taatccacat catccaccgc ttgtgcgggt ccccgtcaat tcctttgagt
     4441 tttaatcttg cgaccgtact ccccaggcgg tcaacttcac gcgttagcta cgttactgag
     4501 gaaatgaatc cccaacaact agttgacatc gtttagggcg tggactacca gggtatctaa
     4561 tcctgtttgc tccccacgct ttcgtgcatg agcgtcagtc tcgtcccagg gggctgcctt
     4621 cgccatcggt attcctccac atctctacgc atttcactgc tacacgtgga attctacccc
     4681 cctctgacgc actctagcct tgcagtcaca agcgccattc ccaggttaag cccggggatt
     4741 tcacgcctgt cttacaaaac cgcctgcgca cgctttacgc ccagtaattc cgattaacgc
     4801 tcgcacccta cgtattaccg cggctgctgg cacgtagtta gccggtgctt attcttccgg
     4861 taccgtcatc cacgccaggt attagccagc gcgatttctt tccggacaaa agtgctttac
     4921 aacccgaagg ccttcttcac acacgcggca ttgctggatc agggttgccc ccattgtcca
     4981 aaattcccca ctgctgcctc ccgtaggagt ctgggccgtg tctcagtccc agtgtggctg
     5041 atcgtcctct cagaccagct actgatcgtc gccttggtag gcctttaccc caccaactag
     5101 ctaatcagac atcggccgct cctattgcgc gaggccttac ggtcccccgc tttcaccctc
     5161 aggtcgtatg cggtattagc taatctttcg actagttatc ccccacaaca gggcacgttc
     5221 cgatgtatta ctcacccgtt cgccactcgc cgccaggccg aagcccgcgc tgccgttcga
     5281 cttgcatgtg taaggcatgc cgccagcgtt caatctgagc caggatcaaa ctcttcagtt
     5341 caacccttca ggagcaccac agcgctgaag cctgggggcg gcggcgcccc acgaggcggt
     5401 gccgggttcg gtcccaggcg gggccaccaa cggacgtgaa gccggtgagc cgctcggggg
     5461 gaagaagagg atcggcgggc ggcgggcggg gaagagggca cagacgggcg agggccgggg
     5521 accgcgaggg caagggcacc cgggagcccg cagaggcggc ggctcgggga gaaacctcag
     5581 gcacggccgg gccaccagga aaacacggcc gcgggatccc accgccacag acacgagggc
     5641 ggtcccgcgg cgccccgcct gggacgcgac gtcaccacat cgatcacgaa gcggtcccag
     5701 gcggggccac caacggacgt gaagccggtg agccgctcgg gggggatc
//
LOCUS       DATMTI010000743         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_792492, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000743 DATMTI010000000
VERSION     DATMTI010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792492"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1879)
                     /locus_tag="VN039_11885"
     CDS             complement(125..1879)
                     /locus_tag="VN039_11885"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HWV46697.1"
                     /translation="MPSHSPFHYGLASGLLVLITVLTAEVILGQVGLPPPIDPSGRSS
                     SPPPVQKEQPLQPKESPAEILPPVEPTPEESNEKGPVLRVFVRKIQVVGNTVLAQEEL
                     RQITATYENREVTTEDLEDVRKLVTMAYVTKGYPNSGAILPDQDLIDGLVTLQVIEGR
                     LSEIRIQGTRWFRPSYLRNRIGLGAGPPLNTTILRNRLQLMLQDERLQQLRTTLTPGT
                     ALGEAVLDVAVVEARPVQMFAEYNNYVSPGVGENQLRGTVVHRNLTGNGDVLSVGFGA
                     SAQAFPFAVGVFPSINVLYLIPLNKYDTTFSVGYRDVKYQVVQDPFKSLDIESKTQIW
                     TASLRQPLYRTLHDDIALTIVGEYEQNANTLSGAPFDSVTGMKNGFGNVAALRFIQEW
                     THRTTASVFAVRSRFTTGIGVLGATVNSMPDAADGQFFSWLGQFQWLRQFESTRIELL
                     HLVNMQMADDRLFPLEQMSVGGRYSVRGYRENTLLRDNGIVYQFETRFPLWSSSTGFP
                     SVQFCPFADVGHSWSAKGSIGDVHTLASIGVGLRFNLTQLTNLNVYWGHRLVTNNVFN
                     PHDSLQDEGVHLQFALNL"
     gene            complement(2050..>3498)
                     /locus_tag="VN039_11890"
     CDS             complement(2050..>3498)
                     /locus_tag="VN039_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46698.1"
                     /translation="DASPTEISSTTSSAGQAGRVHIVTPTMHLDDWAVVSTSSQSTGH
                     AGSVVLDVGRLDLLRGARIESSGLERSGGVVINAAKSITLDKGTITTDALNPGQTGQS
                     GNVSLHAGQSILIGNGSRISADTAKDVSDIVRQDAGNIVIQAGKSVVVRDSTVSARAQ
                     AETGNSGTIVIDAGKNFVSEASTLSVAAASPAGGDSGQIRVTAGDSVRLRNGTVLDGT
                     NAGPGGDGAHIVLDAGKHVVVDASRVITPVLEGGSGRIEMHAGKDVRLVNGTVMDASA
                     GARVPGGQIVVDAGRNILVDASQVRVPVGDGGTGQIVMHAEKDVRLVNGTVLDASSEA
                     SGGGQIVVTGGNVVIDNSRLTADTAAGSFGNAGKVTVIADGSVRLLNGTLVTASSPIF
                     SSGGQVSVTAGDSVQLRNSTIQTSGTFGGGTIVLTSETVRLKNGRLVSSASEDVGGTI
                     TIKTNDFRRDAASVLDVSGSIANGTINIEPLP"
BASE COUNT          757 a         1069 c          914 g          758 t
ORIGIN      
        1 atggtccgat gcttcttctt gtcggcgttg ggtatcatta ccagaggaaa ctattttagg
       61 cttcttacac aatcctcgca acccggatcg aaggcggcat cagcaaaatt cccctcctcc
      121 gttatcagag attgagtgcg aactgcagat gcaccccctc atcttgcaaa ctatcgtgcg
      181 gattgaagac attgttggtg acgaggcgat gcccccagta cacattgaga ttggtcagct
      241 gagtgaggtt gaaccgcaat ccgacaccga tactggccag cgtatgcaca tctccaatac
      301 ttcccttcgc agaccaggag tgtcccacat ccgcaaaggg gcagaactgc acggaaggaa
      361 agcctgtgct ggaactccat agcgggaagc gggtctcgaa ctgatagacg atgccgttgt
      421 cgcgcagaag ggtattttct cgatacccgc gcacactgta ccgtccgcct acagacatct
      481 gctccagcgg aaagagccga tcatcggcca tttgcatgtt gaccaggtgg aggagctcaa
      541 ttctcgtaga ctcgaattgc ctcagccatt ggaactgtcc aagccaagag aagaattggc
      601 cgtcggcagc atccggcata ctgttcacgg tcgcacccaa gacgccgatt cctgtcgtga
      661 agcgggaccg aacggcgaaa acggatgccg ttgtccggtg cgtccattcc tgaatgaaac
      721 gcaaggcggc cacgttccca aaaccgtttt tcatacctgt caccgaatca aatggagcgc
      781 ccgacaaggt gttcgcattc tgctcatact caccgacaat cgtcagcgcg atgtcgtcat
      841 gtagggttcg gtacaacggt tgacgaagcg aggccgtcca gatttgcgtt ttgctctcga
      901 tgtcgagaga tttgaacgga tcttgaacca cttgatactt cacgtcccga tagccgaccg
      961 aaaacgtcgt gtcgtatttg ttcaagggaa tcagatacag cacattaatg ctgggaaaca
     1021 cgccgaccgc aaatggaaac gcttgagcgg aggccccgaa ccccacgctg aggacatcgc
     1081 cgttcccggt gagattgcga tgaactaccg ttccgcgcag ctgattctca ccgacacccg
     1141 gactgacgta gttgttgtat tccgcaaaca tctgtacagg acgagcttcg accacagcga
     1201 cgtcaagaac ggcctcccca agggcggtcc ccggcgtgag cgtcgtccgt aattgttgaa
     1261 gccgttcatc ctgcaacatc aattgcagcc gattgcgaag aatagttgta ttcagaggcg
     1321 gaccagcccc cagaccgatg cggttacgca ggtacgacgg tcgaaaccac ctggtgccct
     1381 ggatccggat ctcgctcagt cgcccttcga tcacctgcaa cgtgacaaga ccatcgatca
     1441 ggtcctgatc cggtagaatg gcgccggagt tcggatagcc cttggtgaca taggccatcg
     1501 tcaccaactt gcgtacatct tcgagatctt ccgtcgtgac ttcccggttc tcatacgtcg
     1561 cggtgatctg ccgcaattct tcctgcgcca gaacggtatt gcccaccacc tgtatcttcc
     1621 tcacaaagac gcgaagcacc ggccctttct cgttcgactc ttcgggcgtc ggttcaaccg
     1681 gaggcagaat ttcggcggga gactctttgg gttggagcgg ttgctctttt tggaccggcg
     1741 gtggcgagct tgaacggccg gacggatcga tggggggcgg caatcccact tgtcccaata
     1801 tgacctccgc cgtcaggacg gtgatcagga ccagtaatcc tgacgcaagt ccataatgga
     1861 aaggagagtg agagggcaaa tcgccacttc cgaaacactc gatactgtcg gcatttttag
     1921 gagaaggacg aaccacttgt aagaatcaag gtgtgcaccg aagccccgac aggcagtccc
     1981 tctccatagt gcaccatgga gagggaaccg cctctcttga ttccccgcct ctttcgaacc
     2041 acattgacac tacggcaacg gttcaatgtt gatggtcccg ttcgctatac tcccactgac
     2101 gtctaagaca cttgcggcat cgcgacgaaa gtcattcgtc ttaatggtga ttgtgccgcc
     2161 aacatcctcg ctggcactac tgaccagccg cccattcttc agtcgtaccg tctctgatgt
     2221 gaggacgatg gtgccgcctc cgaaggtgcc ggaagtttgg atcgtgctgt tgcgcagctg
     2281 caccgaatcc ccggccgtaa cgctgacttg gccgccggaa ctgaatatcg gactcgacgc
     2341 ggtcacgagc gttccattga ggagacgcac ggatccatca gcaatcacgg tcaccttccc
     2401 ggcgtttccg aaactgccgg cggcggtatc agccgtcaag cggctattat cgatcaccac
     2461 gttaccgcca gttactacaa tctgtccccc gccactggcc tcgctacttg cgtccaagac
     2521 tgtcccgttc acaagtcgga cgtccttttc ggcatgcatc acaatctgcc ctgtgccccc
     2581 gtctcctacc ggcaccctca cctggctcgc atcgacaagg atgttgcggc cggcatcaac
     2641 cacaatctgc ccccctggaa cgcgggcgcc ggcacttgca tccatgaccg tcccgttcac
     2701 aagccgcacg tcctttccgg catgcatctc aatccgcccc gatcctccct caaggactgg
     2761 ggtgatcaca cggctggcat ccacgacgac atgcttgccg gcgtcgagca cgatatgagc
     2821 cccatccccg ccgggtccgg cattggtccc atccaggacc gtgccgttgc ggagccgcac
     2881 tgaatccccc gccgtcacgc gaatctggcc actatcaccg cccgcaggtg atgcggcggc
     2941 gactgataga gtgctcgcct cactgacaaa attcttgccc gcatcgatca cgatggtccc
     3001 gctattgccc gtttcagcct gagccctggc cgagacggta ctatcgcgga cgacgacact
     3061 tttgcccgct tgaatcacga tattgccggc atcttgcctc acgatatcgc tgacatcctt
     3121 tgcagtatca gcagagatac ggctgccatt gccaatcagg atggattgtc cggcgtggag
     3181 gctcacattc ccactctgtc cagtttgtcc ggggttaagc gcatcagtgg tgatggtgcc
     3241 cttgtcaagg gtaatgctct tggcagcatt gatcacaaca ccaccacttc tttccagacc
     3301 actgctttcg attcttgcgc ctcgtaggag atccaaacga cctacatcca gaacaacgct
     3361 gccggcatgg ccggttgatt ggctgctggt gctcacaacg gcccaatcat ccagatgcat
     3421 ggtcggtgtc acaatatgaa cacgccccgc ctggccggct gaagacgtgg tactgctgat
     3481 ttcggtgggg gaagcatc
//
LOCUS       DATMTI010000744         5536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_155379, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000744 DATMTI010000000
VERSION     DATMTI010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155379"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..667
                     /locus_tag="VN039_11895"
     CDS             146..667
                     /locus_tag="VN039_11895"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWV46699.1"
                     /translation="MDEVIHILNSEELKQPGRSVERRQKIEQVIRQRVSYEDMAKRAL
                     GWPWIELTNSERQEFVALFVQLLRDTFACRIDNYADEQVLYLSEQREENVAEVKAKLS
                     GLKVNTLLDFRLVDKVGHWYVYDVIIDGAGIVGNYHAQFSSIIRDHSYAGLVNKMKEK
                     TLVVKAFETATDP"
     gene            725..>5536
                     /locus_tag="VN039_11900"
     CDS             725..>5536
                     /locus_tag="VN039_11900"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWV46700.1"
                     /translation="MNTMFSPVAKSAELEEPIRAELFGIERLEQHAASLAVAQVVTED
                     ARVGRLLTPRVLENGRVLVSSYQTIARTIRDEKTITPAAEWFVDNFHIVDEQLREIID
                     DLPPGYYRQLPKLASGHLQDHPRVFGVAWAFVAHTDSRFDPDMLRRFVMAYQRVQPLT
                     IGELWAVAISLRIVLVENLRRLTERIVHSREARLEADELADSLLGSGALALESPAMAL
                     RRFEQSPLDTAFAVQLVQRLRDLDPKVRPMLRWLDERLAAQGATADDMVHVEHQQQAA
                     MSVTVRNIITSMRLTSEFNWAEFFEAVSPVDKILQCDSRYAEMDFVTRDTYRHAIEDL
                     SRGTGFSEIDVTTRVMDRARLAGIEPHASEQPERDRHTDPGYYLISQGRRAFEHELGY
                     RVSWKRRLLRWYVQASATGYLGSLAAVTLMVLALPLWHASEAGVTLVGLVCLGLIALV
                     PASDLAMALINRTVMAILRPRSLPRMALRNGIPKELRTIVVVPTLLASRQGIDELVER
                     LEVHYLANADDDLRFALLSDWRDAPSESIPGDAELLAIAVEGIAHLNKRYGPAAGGGS
                     RFVLLHRRRVWNESEQVWMGWERKRGKLHELNQWLRGSTSTTFMSVGGQPPEAIPSVR
                     FVITLDADTRLPRGAAHRLIGTMAHPLNRPRFDPRAGRVLEGYGVVQPRITPSLPGSG
                     EGSYFQQTFSGPSGIDPYASAVSDVYQDLFREGSYTGKGIYEIDAFEAALADKVPDNA
                     MLSHDLFEGLFARAALATDIELFEAFPSHYEAAAARQHRWARGDWQLLPWLFGRGHTG
                     SEPHRAVVIPAIGRWKILDNLRRTLSAPAAFLTLIVGWMQPEQSAWVWSGFILITIAI
                     PSLLSFVLGLYPRRSGIALRSHFRGAGTDLKLAARQIALTVTFLAHQAWLMTDAILRT
                     LVRLVFMHTRMLEWVTAAQAEDAYTCNLTGMYRRMAGGVVLAFVAAGAVAVFGHDESW
                     AAAAPFVLLWMSAPAVARWVSLPPRLTGIGPISPADARTLRLIARRTWRFFETFVSSE
                     DHALPPDNFQEDPKPVVAHRTSPTNIGLYLLSTLVARDLGWLGTLDATERLEATLATM
                     NRLEVFRGHFYNWYDTRTLFPLDPKYVSSVDSGNLAGHLLVVGNGCRELIQQSSINGD
                     LFAGIDDAIQLLRDALEKIPDQRQAHTVTRKQLSQAVDVLAMALLSRPIDAAGWTARF
                     SRLTTLSHTVSDMAQALGQERGDEPDGELRTWADAVRACVESHLRDAELLLPWVRFSA
                     KDRAAMFDHPSGPSPEWMVIEPFFHPIPTLAAAPARFAAALRTIENLREQLLSRPSQD
                     WDTLARITLLADAIRQSVVDITALARRLTAIAQNAERMVHAMDFTFLFDPTRKLLSIG
                     YRVPENSLDPSCYDLLASEARLSSFIAIAKGDVPSSHWFHLGRALTPVGDGSALVSWS
                     GSIFEYLMPALVMRFPAGSLLCRTYELIVRRQIQYGRERGVPWGVSESAYNARDLDLT
                     YQYSSFGVPGLGLKRGLSEDVVVAPYATALAAMINPMEATQGFASLAEAGGRGTYGFY
                     EALDYTGTRVPEGKDVAIVRAYMSHHQGMSLVSIANVLNNGVMRSRFHGEPI"
BASE COUNT         1052 a         1546 c         1727 g         1211 t
ORIGIN      
        1 cagcagtgcc gctagtacgg gattcatcag ttccgaaacg cgtctcggca tgatctgtgg
       61 ggtgatggcc ggcatggttg tgctgctgat cgggactccc gtggcttcag ctgagtagac
      121 cgccacagaa tcggtcaaga gcaccatcga tgaggtgatc catattctca acagtgaaga
      181 gctgaagcag ccaggacgct cggtagaacg acgtcagaag attgagcaag tcatcagaca
      241 gcgtgttagc tatgaagaca tggccaaacg ggccctgggc tggccctgga tagaactgac
      301 taacagcgag cggcaggaat ttgtcgccct ctttgttcag ttactccgag acacgtttgc
      361 ctgtcgaatt gacaactacg ccgacgagca agtgctctac ctgtctgaac agcgggaaga
      421 gaacgttgcc gaggtgaaag cgaagctgtc gggccttaag gtgaataccc tcctggattt
      481 tcgcctcgtc gacaaggtcg ggcattggta tgtgtacgat gtgatcatcg atggcgctgg
      541 gatcgtaggc aattaccatg cgcagttctc cagcattatc cgcgaccatt cctacgcagg
      601 tttggtgaac aagatgaaag agaaaacact tgtggtgaaa gcgtttgaaa cagccacgga
      661 tccatagttg gtgtacttca gcaactgagt cagcctggcg actccggagt tggttggaaa
      721 ccctatgaac acgatgtttt cacctgtcgc caagagtgcc gagctggaag aaccgattcg
      781 cgccgagctt ttcggcatcg aacggctcga acagcatgct gcaagcctcg cagtggcaca
      841 ggtcgtcacc gaagatgcac gggtgggccg gttactcacg ccacgagtcc ttgagaatgg
      901 acgggtcttg gtgtcgtcgt atcagactat cgcgcgtacg atccgcgatg agaaaacgat
      961 taccccggcg gctgaatggt tcgtcgataa tttccatatt gtcgacgaac agcttcgaga
     1021 gatcatcgac gatttaccgc ctgggtatta caggcaattg cccaaattgg catccgggca
     1081 tttacaggat catccacggg tctttggagt tgcctgggca ttcgttgccc acaccgacag
     1141 tcgtttcgac cccgacatgc tccgtcgctt tgtgatggcg tatcaacgcg tgcaaccgct
     1201 gacgatcggt gaattgtggg cggtggccat ctcgctacgt attgtgctgg tggagaatct
     1261 tcggaggctg accgagcgga ttgtccatag ccgcgaagcc cgcttggaag ccgatgaact
     1321 tgcagacagc ttgttgggga gcggggcgtt ggctctggag tcaccggcca tggccttacg
     1381 ccggtttgag cagagcccgt tggacacggc gtttgccgta cagctggttc aacggctccg
     1441 cgacctggac ccgaaggtgc gtcccatgct gcggtggctc gatgaacgcc tggccgcgca
     1501 gggcgcgacc gctgatgaca tggtgcatgt cgagcatcag caacaggcgg ccatgagcgt
     1561 cacggtccgc aacatcatta cgagcatgcg gttgacgtct gaattcaatt gggcggaatt
     1621 tttcgaggcg gtcagtccag tcgataagat tctgcaatgc gactcccgct acgccgagat
     1681 ggactttgtc acccgggaca cataccgcca tgcgatcgag gatctctctc gcggcacggg
     1741 cttctcagaa attgacgtga ccacgcgggt catggaccgt gccaggttgg ctgggattga
     1801 gccgcatgcg agtgagcagc cggagcggga tcggcacacg gatcccggct attaccttat
     1861 ctctcagggt cgccgggcat ttgaacacga actcggctat cgggtgtcct ggaaacgacg
     1921 gctgctgcgc tggtatgtcc aagcttcagc aacgggctat ctgggatcgc ttgcagcggt
     1981 gaccctcatg gtcctggcat taccgctgtg gcatgcctcc gaagcgggtg tgaccctcgt
     2041 gggcctggtg tgtctcggcc tgattgcgct cgtacccgcc tcggatctgg ctatggcgct
     2101 tatcaatcgt acggtgatgg ccatcctcag gccgcggtca ttgcctcgga tggcattgcg
     2161 gaacggcatt cccaaagaat tgcgcacgat cgtggtggtc cccacgttgc tggcgagccg
     2221 gcagggaatc gatgagctgg ttgaacgatt ggaagtgcat tatctggcga atgcggatga
     2281 tgacctgcgc tttgctctgc tctcagactg gagagatgcc ccgagcgaaa gcattccagg
     2341 ggatgccgaa ctgctggcca tcgcggtgga ggggatcgcg catctgaaca agcggtatgg
     2401 ccccgcggcc ggcggcggtt cgcgtttcgt gctgttgcac cgcaggcgag tctggaacga
     2461 gtctgagcag gtgtggatgg gatgggaacg gaaacgaggc aaactgcatg agttgaatca
     2521 atggctacgc ggctcgacga gcacgacgtt catgtccgtc ggcgggcaac cccctgaggc
     2581 gattccgtcg gtccggtttg tcatcacatt ggatgcggat acacggttgc cccgtggagc
     2641 cgcacaccgg ttaatcggaa ccatggctca ccctctgaac cggccccgat tcgatccgcg
     2701 cgccgggcgt gtgcttgaag ggtatggagt cgtgcagccg cggattacac cgtcgctccc
     2761 gggcagcggt gagggctcgt attttcaaca gacattttcc ggtcccagcg ggatcgatcc
     2821 ctacgcgtcg gccgtctccg atgtgtatca ggacttgttt cgggaaggat cctacacagg
     2881 caaaggcatt tacgagatcg acgcgttcga ggctgcgctg gcagacaagg tgccggacaa
     2941 tgcgatgctc agtcacgatc tcttcgaagg gttgttcgcg cgcgcggcct tggcgaccga
     3001 catcgagctg ttcgaagcgt ttccttccca ctacgaagcg gcggcggcca ggcagcatcg
     3061 atgggcgcgt ggcgattggc aattgctgcc ctggctgttc gggcgagggc acaccgggtc
     3121 tgagccgcat cgcgcggtgg tgattcccgc catcggccgt tggaagatcc tcgacaacct
     3181 ccgccgtacc ctgtcggctc ccgcggcatt tctgacattg atcgtcggat ggatgcagcc
     3241 ggaacaatct gcctgggtct ggtctgggtt cattctgatc accatcgcga ttccttcgct
     3301 gttgtccttt gtgctcgggc tctatccgcg ccgatccggg attgccttgc gcagtcattt
     3361 ccgtggagca ggcaccgacc tgaagttggc cgcgaggcag atcgcgttga ccgtcacttt
     3421 cctcgcgcat caggcatggc tcatgacgga tgccattctt cgtacactcg tccggctcgt
     3481 cttcatgcac acccggatgt tggagtgggt gaccgcggct caagccgagg atgcttacac
     3541 gtgcaacttg acggggatgt atcgtcgcat ggcgggcggt gtggtgctcg ccttcgtggc
     3601 agctggagca gtggccgtgt ttgggcacga tgagtcctgg gctgccgcgg ctccgtttgt
     3661 tctgctgtgg atgtcagctc ctgccgtggc acggtgggtc agtctgccgc ctcgactcac
     3721 ggggatcgga ccgatttctc cggccgacgc gcggacgctc cgattgattg cacgccggac
     3781 ctggcggttc ttcgaaacgt tcgtgtcttc cgaggaccat gcgctgccac ctgataactt
     3841 tcaagaggat ccaaaaccgg tcgtagccca ccgcacatcg ccgaccaaca ttggactcta
     3901 tctgctctca accctggtcg cccgtgacct cggatggttg gggaccctcg acgccaccga
     3961 acggctggaa gccacgctgg cgaccatgaa ccgtctcgag gtgtttcgtg ggcatttcta
     4021 caattggtac gacacacgta ccctttttcc gctggatccg aaatatgtgt cctccgtcga
     4081 tagtggaaat ctcgcgggac atttgctggt cgtggggaac ggatgccgcg agttgattca
     4141 gcaatcttcc atcaacggcg atttgtttgc cgggatcgat gatgccatcc aactgttacg
     4201 cgatgcattg gagaagattc cagaccagcg acaggcccat acggtgacca ggaagcagct
     4261 gagccaagcc gtcgatgtgc tggccatggc gctcttgtcg aggccgatcg atgccgccgg
     4321 ctggaccgcc agatttagca ggctcaccac cttgtctcat acggtctcgg atatggctca
     4381 ggcactggga caggagcgcg gggatgagcc cgacggggag ttgcgcacct gggccgacgc
     4441 ggttcgggcg tgcgtggaaa gccacctccg ggacgcagaa ctgttgctgc catgggtccg
     4501 cttcagtgcg aaagaccggg ctgccatgtt cgaccatccg tcgggaccat cgccggagtg
     4561 gatggttatc gagccgttct ttcatcccat tccgaccctg gctgccgcgc ctgcgcggtt
     4621 cgcggccgcg ctccgtacaa tcgagaattt acgtgaacaa ttactcagtc ggccatccca
     4681 ggattgggac accttggcaa gaatcacctt gctggctgat gccatccggc aatcggtcgt
     4741 ggatatcacg gccttggccc gtcgtctcac ggcgatcgca caaaacgcgg agcgcatggt
     4801 tcacgccatg gacttcacat ttctcttcga cccgacacgc aaactgttgt ccatcggcta
     4861 tcgcgtgccg gagaatagcc tggaccccag ttgctacgac ttgctggcgt cggaggccag
     4921 actgtcgagc tttatcgcca tcgccaaggg cgacgtgccg tcatcgcact ggtttcactt
     4981 gggccgtgcc ctgacacccg tgggagacgg ttcggcgctc gtgtcctggt cgggctccat
     5041 ctttgaatac ctcatgccgg cgttggtgat gcgtttcccg gcaggcagct tgctatgccg
     5101 gacgtatgag ctgatcgtcc gtcggcagat tcaatacggt agagagcgag gtgtgccatg
     5161 gggtgtttcg gagtcggcct ataatgcgcg tgatctcgac ctgacgtacc agtattccag
     5221 ctttggtgtg ccgggtctag gactcaagcg aggcctcagt gaggatgtgg tcgtcgcgcc
     5281 ctatgccact gcacttgcgg cgatgatcaa tcccatggaa gccacgcaag gtttcgcgag
     5341 cctcgccgag gcgggaggga ggggaactta cgggttctat gaagctctgg attacactgg
     5401 gacacgggta ccggaaggaa aagacgtggc catcgtgcgg gcctatatgt cgcatcatca
     5461 ggggatgtcg ttggtttcga tcgcgaatgt tttgaacaac ggagtgatga ggagccgttt
     5521 tcatggcgag ccgatc
//
LOCUS       DATMTI010000745         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_282308, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000745 DATMTI010000000
VERSION     DATMTI010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282308"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            578..751
                     /locus_tag="VN039_11905"
     CDS             578..751
                     /locus_tag="VN039_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46701.1"
                     /translation="MTRDKSQLVQLPLAVIMFVLVLGALSMIWPAIIALFLGVLLLLL
                     QPFSGELGVVVLP"
     gene            995..>1650
                     /locus_tag="VN039_11910"
     CDS             995..>1650
                     /locus_tag="VN039_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWV46702.1"
                     /translation="MSTAQDYISHPEVLKALQTVKSECRGKQYSDIIDAGDHQYVDLV
                     MEGGGVLGIALTGYTYVLEQAGIRFLGVGGTSAGSINALMIAALGPPGQAKSERLLDV
                     LSRMPMETFIDGDSDARDFSQAVLEKAGMVKLIWKASQIIDNLKEDLGLNPGDAFLNW
                     LTSELQGVGIKTTAGLLKQLRTVSPGLRLRPTRTDTELSEKDKDGDLVMIAADITTQT
                     "
BASE COUNT          394 a          414 c          465 g          377 t
ORIGIN      
        1 gactacctgg tgggcctctg cgccaaggcc ggtctgactg tggcagtcag gttagccgca
       61 tagatgaatg cttcatttca aagcccgacg ttgaacctag agggatatga aaagccgcgg
      121 tcataaagat tctgtcctac atgctatatc gagctcagaa tgacagcgat cccgatagcg
      181 taacgaccat tacattcacc tcttggctga tcttcttttg ctcaatcggc caactgctca
      241 accgctccat cctcatgttc tgaatccaat ccgatccaag tgcaatccat tcagatacag
      301 atgcgcgctg tccaacttcg aaacagctcg ctcagattat tggaatatct tggctttcta
      361 tgtgaaacgc gtgtggcgca aaaagtgctt cgtgatcgat gtgctcatga acgaacagaa
      421 tgtcgaggag gtgtaaacgg tctcggcttc cacgcttcga acccgatcaa ggcacggggg
      481 tgacgatgtt gagtaggtca caagtacacg cggtgagcgg cgatacgatt tgtgtttgag
      541 gcgtgaccca ggcaaaacgg atgtcatgga ggacccaatg accagagata aaagtcagct
      601 cgtccagctg ccgttagcag tgatcatgtt cgtcctggtt ctcggtgctc tatcgatgat
      661 ctggcccgcc atcatcgcgc tatttctagg cgtactacta ctgctgctcc aacctttcag
      721 cggcgagctg ggggtggtgg tactgccata ggcagaggta agcaatcttg cgatcgccct
      781 tcgtttctct tcggcagcgg gtttgtcgat tgccgatgag atcaggtcga gtcctttgtg
      841 gacctctcga agaggggtcg tgatgctcgg atcatgcacc aacgcttgaa ctcgtgtcga
      901 gaggggaaat cgattacact cggacctcgg gtagcagcaa tacggccata gaaagaacat
      961 ttataccgta taccgtatct ttacttggaa gaccatgtcg acagcacaag actacatcag
     1021 ccatccggag gttctgaagg cactgcaaac ggtgaagagc gaatgcaggg gcaagcaata
     1081 ttccgacatc atcgatgcgg gcgatcatca gtacgtcgat ctggtcatgg aaggtggagg
     1141 tgtgctcggc atcgcgctga cagggtacac ctacgttctt gaacaggcag gcatccggtt
     1201 tctgggcgtg ggtgggacat cagcgggatc catcaatgcc ctgatgattg ccgcgttggg
     1261 tcctccgggg caagccaaga gcgagcgcct gttggatgtc ctttcacgaa tgccgatgga
     1321 gacctttatc gacggcgata gcgacgccag ggatttttca caagcggttc tggagaaggc
     1381 cggcatggtc aaattaatct ggaaggcctc tcagattatc gacaacctga aggaagattt
     1441 gggcttgaat ccaggagacg cgtttttgaa ttggctgacc tccgagctgc aaggagtcgg
     1501 aatcaaaacg acggcggggc ttcttaagca gttgcgcacc gtttccccgg gactgagact
     1561 gagaccgacg cgcaccgata cggaactgtc cgagaaggac aaggacgggg atttggtcat
     1621 gatcgccgcc gatatcacca cccagacaaa
//
LOCUS       DATMTI010000746         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_28276, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000746 DATMTI010000000
VERSION     DATMTI010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28276"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..252)
                     /locus_tag="VN039_11915"
     tRNA            complement(176..252)
                     /locus_tag="VN039_11915"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:His,seq:gtg)
     gene            complement(293..>1577)
                     /locus_tag="VN039_11920"
     CDS             complement(293..>1577)
                     /locus_tag="VN039_11920"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWV46703.1"
                     /translation="LSGSINVKTSKNAAVGLLCASLLNKGKTTLRRVARIEEVNRLIE
                     VLNSIGVKTRWLENNDLEIIPPKRLQLENIDTAAAKRTRSIIMFLGPLLHQYDDFKLP
                     FAGGCNLGTRTVEPHMVGLAPFGLSVEARPNTDYYHATVEPKSLERTILLTERGDTVT
                     ENVVMAAALYNGTTVIRNASPNYMVQDVCFFLKKLGVKIEGIGTTTLTIKGVSSIDKN
                     VEYFPSEDPIEAMSLIAAAVVTQSELTIKRAPIEFIEIELAILRGMGLDFSLTPEYLA
                     DNQATRLVDITVKKSNLVAAKDKLHSMPFPGVNMDNLPFLGLIAAVAKGRTLVHDWSY
                     ENRAIYFTELTKLNAKVELVDPHRVYLTGPTKWKPADLIAPPALRPSVVILLAMLAAP
                     GISTLRDVYSINRGYEDFANRLTSIGAQVETLQEI"
BASE COUNT          367 a          392 c          448 g          370 t
ORIGIN      
        1 cacggcagga gttgaatgcc aaaaatccgc accctgtcgc cacaccgggt aacatcatcc
       61 agcctccggc tcctgcgggc agcaaaccac agttcgtcga gccgacgacc cgcggttaca
      121 atccgtacgc atagcgagca aataaaaaac ccatctgttt gatgggtgtt ttatttgggg
      181 cgaatgatgg ggattgaacc cactaccttc ggtaccacaa accgacgctc taaccaaatg
      241 agctacattc gccacacgca cagcttatag tagcatattt aacagatgaa aactagattt
      301 cctggagagt ttcgacctgc gcgccgatgg aggtgaggcg gttggcgaag tcttcatagc
      361 cacgattgat cgagtagaca tcacggagcg tcgaaatacc gggtgcggca agcatcgcaa
      421 gcaggataac taccgatggg cggagtgctg gtggtgcgat aagatcggca ggcttccatt
      481 tggtaggtcc ggtcaaatag acacggtgcg gatcgaccag ttcgactttg gcgttgagct
      541 tggtgagttc cgtaaagtaa atggcacggt tttcgtatga ccagtcatgc accagtgtgc
      601 gacctttggc aacggcggcg ataaggccga ggaagggtag gttgtccata ttcacgccag
      661 ggaaaggcat actgtggagc ttatctttgg ccgcgacaag gtttgatttc ttgacggtga
      721 tatcgacaag acgggtcgct tggttatctg ccaaatactc cggcgtaaga gagaaatcga
      781 ggcccatacc gcgcagaatg gccagttcaa tctcgatgaa ttcaatcggc gcacgcttga
      841 tggtgagctc cgactgcgtg acgactgcgg cggcgataag actcatcgct tcgattgggt
      901 cttcactcgg gaagtactcc acattcttat ctatggaaga gacacctttg atggtaagtg
      961 tcgtcgtacc gataccttca atcttgacgc caagcttctt caggaagaaa caaacatcct
     1021 gaaccatgta gttcgggctg gcgttgcgga tgacggtcgt gccgttatac agggcggccg
     1081 ccataacgac gttttcggtt acggtgtcac cacgctcggt caggaggatc gtccgctcga
     1141 gagatttcgg ctctacggtg gcatggtagt agtcggtatt cggacgcgct tcgactgaca
     1201 ggccaaatgg cgccaagccg accatatgcg gctcgacggt acgtgtgcca agattacagc
     1261 caccggcgaa cggcagttta aagtcgtcat attggtgcaa cagtggccca aggaacataa
     1321 tgatactgcg tgtccgtttg gcagcggcgg tatcgatatt ttcgagctgc aggcgtttcg
     1381 ggggaatgat ctcgaggtca ttattttcca gccagcgtgt tttgacgccg atactgttca
     1441 atacttcaat caggcgattg acctcttcga ttctggctac ccgcctgagg gttgtcttgc
     1501 ctttgttgag taagctggcg cacagtagcc cgaccgccgc gttcttgctc gttttgacgt
     1561 tgatagagcc ggaaagc
//
LOCUS       DATMTI010000747         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_664679, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000747 DATMTI010000000
VERSION     DATMTI010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664679"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /gene="ftcD"
                     /locus_tag="VN039_11925"
     CDS             complement(<1..1379)
                     /gene="ftcD"
                     /locus_tag="VN039_11925"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HWV46704.1"
                     /translation="MDRLVECVPNFSEGRDRETLHALIDAVASTAGVALLDHSMDADH
                     HRSVLTFCGSPDAIVEAAFRAVRIATELIDLRTHIGVHPRIGATDVVPFVPIRGMTMQ
                     DCVRLAKQLGERVGRELGIPVFLYEYAAGRADHVPLEAVRRGGLDGLAFRMESDPGWI
                     PDFGPPRLHQSAGAIAIGARPPLIAYNVNLCSTEVDQARSIARAVRHSNGGLPFVKAI
                     GVELSSRGMVQVATNLTDYLVTPILTAFQKVKAEATKRGIEVAGSELVGLVPQAALSQ
                     AAAASLQLERFDSSQVLETRIVEAMLSKKEPALTLSDFLAAVADAKPTPAGGSVAAFV
                     GALAASLGVMGARLGGQSDRVQRLLELSEQLYRLVQADTEVYRGLMDAYKMPKQHPDR
                     PHAISIALQRATEVPLEITELSCEVAQLLHTLRDGAKPSIHSDLTVGLTIAIAAARAG
                     LVTARTNTNV"
BASE COUNT          321 a          462 c          427 g          350 t
ORIGIN      
        1 acatttgtgt ttgtgcgggc tgtaacaaga cctgctcgcg cggctgcaat ggctatggtc
       61 aaacccactg tgagatctga atggatgctc ggcttagctc catcgcgcaa cgtatggagg
      121 agctgcgcaa cctcgcagga cagctccgtg atttctaatg gcacttccgt agcccgttgg
      181 agtgcaatcg agatcgcgtg aggacgatca ggatgttgtt tgggcatctt gtaggcgtcc
      241 atcagccccc tgtagacctc cgtatctgct tgaacaagcc gataaagctg ctcgcttaac
      301 tcaagcagac gctgcactct gtccgattgt ccaccgagac gtgcccccat gacccctaaa
      361 ctcgccgcta aggcacctac gaaggctgcc acgctgccgc cggccggtgt cggcttggcg
      421 tcggcaaccg cggcaaggaa atctgagagg gtgagagccg gttccttctt gctgagcatc
      481 gcctcgacga ttcttgtttc gaggacctgg cttgaatcga atcgctcgag ttgcagcgaa
      541 gcagcggcag cctgactcaa tgccgcttga gggaccaagc cgaccagttc gcttccggcc
      601 acttctattc ctcgcttcgt ggcttcagcc ttgaccttct gaaatgcggt cagaatgggc
      661 gtcacgaggt agtcggtcaa attcgttgca acctgcacca taccgcgact ggatagttct
      721 acaccgatcg ctttcacaaa gggtaacccg ccgtttgagt ggcggactgc gcgggcgatg
      781 gatcgggcct ggtcaacctc cgtcgagcag aggttcacgt tgtaggcaat cagtggaggc
      841 cgggcgccga tcgcgatcgc tcctgcgctt tgatgtagac gagggggacc gaagtccgga
      901 atccaaccag ggtctgactc catccgaaaa gccaggccgt cgagccctcc tcgtctgacc
      961 gcctctaatg gaacgtggtc ggcacgacct gcagcatatt cgtataagaa aacggggatc
     1021 cccaattcac gcccgacgcg ttctcctagc tgtttggcaa gccggacgca gtcttgcatt
     1081 gtcatacccc tgatcgggac aaacggcacc acatctgtcg ctcctatgcg ggggtgtact
     1141 ccaatatgtg tacgcaggtc gatgagttca gttgcgatcc gaaccgcacg aaaagcggct
     1201 tctactatag cgtccgggct tccacagaac gtcagcaccg accggtggtg gtccgcatcc
     1261 atcgaatggt ctagaagtgc gacaccggct gtcgaagcca cggcatcaat caaggcgtga
     1321 agagtttctc gatcccgacc ttcactgaaa ttggggacac actcgacgag ccgatccata
     1381 ggtttctgca cgtattaacg aaaaagccgg aggaccgatc accgttcctc cggcttctgg
     1441 cttacccgtc tctctgacgg gagtctggct atttcgcctt tttaacgaac gccttataga
     1501 tgcggcccga ctttgcttgt tcctcttcca accccatcat ttcatggcct gtcgtttcac
//
LOCUS       DATMTI010000748         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_538004, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000748 DATMTI010000000
VERSION     DATMTI010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538004"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VN039_11930"
     CDS             complement(<1..1226)
                     /locus_tag="VN039_11930"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46705.1"
                     /translation="MALAQPPKTQADNVPSSGKFQYPGIPGTADGSGMVVHVETRACE
                     AGIAYPITPSTSMGVGYQTAFSNGMKNLWGKDIMWLQPESEHSSASASEGYAVAGGRV
                     TNFTSGQGLVLMKEVLYTISGKRLGLVFNIGARALTSHSLNVHAGHDDVMSVTDVGWG
                     MLFAQNAQEAGDLCLISRRTAEAAKTPFMNIQDGFLTTHTIENVLFPEDDMIREYLGR
                     PDDKLRKLFDPRFPLMSGVVQNQDSYMTGKIAQRLFYDRIPELLQESMDEFYRLTGRR
                     YNMVTPFQLEDAEYAIVSMGSTAETASVTLDHLRSKGHKTGSLTITSFRPFPGPQIVE
                     ALKNVKAFAVIERMDNPLGVSNPLTAEIEAAFAKAMMGTPGYPKIDRVPVIYSGVAGL
                     GSRDVTPGQMVATVDN"
BASE COUNT          319 a          382 c          478 g          349 t
ORIGIN      
        1 atgttgtcca cggtggcgac catttggccc ggagtcacgt cgcggctgcc cagaccggcc
       61 acaccggagt agatcaccgg cacgcggtcg attttggggt aaccaggggt gcccatcatc
      121 gctttggcga aggccgcctc aatttcggcg gtcagcgggt tggaaacgcc cagtgggttg
      181 tccatccgct caatgacggc gaaggctttc acgttcttca gggcttccac gatttgcggg
      241 cccgggaacg gacggaagga ggtgatggtc aggctaccgg tcttgtggcc cttgctgcgc
      301 aggtgatcca aagtcacgga agctgtttcc gcggtggaac ccatggagac aatggcgtac
      361 tcggcgtctt ccagctggaa gggggtgacc atattgtaac ggcgaccagt caggcgatag
      421 aactcatcca tggactcctg caacagctcg ggaatccgat cgtagaagag acgttgagcg
      481 attttaccgg tcatgtagct gtcctggttc tgcaccacgc cgctcatcag cgggaatctg
      541 ggatcgaaca gcttgcgcag cttgtcgtcg ggacggccta ggtattcgcg gatcatgtcg
      601 tcttccggga agaggacgtt ttcaatggtg tgggtggtca aaaagccgtc ctggatgttc
      661 atgaagggcg tttttgcagc ttcggcggta cggcggctga tcaggcacaa atcgccagct
      721 tcctgagcat tctgggcgaa tagcatcccc cagcccacgt cggtgacgga catcacgtca
      781 tcgtgaccgg cgtgcacgtt cagagagtga gaggtcaagg cgcgggcgcc gatgttgaag
      841 accagaccca ggcgtttgcc ggagatggtg tagagcactt ccttcatcaa taccagacct
      901 tggccggagg tgaagttggt gacgcggcca ccggccacgg cgtagccttc cgaagcagag
      961 gcagagctgt gctcggattc cggttggagc cacatgatgt ccttgcccca caggttcttc
     1021 atcccgttgg agaaggcggt ctgataacct acgcccatac tggtggatgg ggtgatggga
     1081 taggcaatac cggcttcgca agcgcgggtt tccacatgca cgaccatacc ggagccgtca
     1141 gcggtgccgg gaatgccggg gtactggaac ttcccggagc ttgggacgtt gtctgcctgg
     1201 gttttcggag gttgggcaag agccatagaa acgcttctcc ttagtttgat agggtgaaaa
     1261 actcgatact gagactcagt tgagagggta aatcatagaa atgcctgagg gatatgtatt
     1321 ttcctgggat agtcagcaaa acgccttact gcgagctttt ttctgatgag gcggggatgg
     1381 caactcgtat ggtcagactt ctcagttgaa cttattgtac aaaaaacgtg gcattttcaa
     1441 agcaagtctt acaaaactgt gcgttttagt tccctgtttg gctgtttttt gcggggatgg
     1501 cctgaaaccg ggtaaaccct tgaattga
//
LOCUS       DATMTI010000749         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_706224, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000749 DATMTI010000000
VERSION     DATMTI010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706224"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VN039_11935"
     CDS             complement(<1..537)
                     /locus_tag="VN039_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46706.1"
                     /translation="MVNLVSATLQLYRHVLYATGRSFARSWVTVVALIVFAILFLGTS
                     RIAAPFGIGGGFILGIVNALLVGATLRLIEQSLSGARSIQLRDVTESFGHYFWDVIGV
                     GFVLWLPTMLLDMGMQANPYGQFLLSAFLLLVFILLNPAPEVIYQVRHDSTLNVFKTS
                     YEFVLEHWVEWFLPFAILI"
     gene            705..>2405
                     /locus_tag="VN039_11940"
     CDS             705..>2405
                     /locus_tag="VN039_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWV46707.1"
                     /translation="MDVNRMTIKLQEALQSASAHAQRRSHQGIDVEHMLLALIDQEGG
                     TAPALLEGAGLALPAVRQAVEQALAKLPQVQGSGAAPGQMHIATRLTQVLNHAEDEQK
                     SLKDDFLSVEHVLLAMVQEGGVLKKLGLTRDRLLSGLQQVRGNQRVTSQDPESTYQSL
                     VKYGRDLTQLAGQGKLDPVIGRDDEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AIRIVKGDVPESLKHKKLFALDMGSLVAGAKFRGEFEERLKAVLKEIQSSQGQILLFI
                     DELHTVVGAGAAEGAMDAANLLKPMLARGELHLIGATTLDEYRKHIEKDAALERRFQT
                     VLVDQPSVENTISILRGLKERYEVHHGVRIKDSALVAAAKLSHRYISDRFLPDKAIDL
                     VDEAAARLRTEIDSLPAELDEVSRKVLQLEIEREALRKEKDPASAARLTALETELNEK
                     QRDLQTLKTRWESEKTSVSRLRKTREAIEDVKLKIDQAERAYDLNRVAELRYGELPRL
                     ERELELEQQHLGKKQDETKLLKEEVDEDEIAAVVSRWTGIPVSRLLEGESDKLLKLED
                     L"
BASE COUNT          601 a          665 c          703 g          436 t
ORIGIN      
        1 gatgaggatc gcgaagggga ggaaccactc gacccaatgc tccaacacaa actcatagga
       61 ggtcttaaac acgttgagcg ttgaatcatg gcggacttga taaatcacct ccggcgccgg
      121 attcaagagg atgaagacca gaagcagaaa cgccgacaac agaaactgcc cgtaggggtt
      181 cgcttgcatc cccatatcga gcagcatcgt cggcagccac agcacgaatc ccacgccgat
      241 cacgtcccag aaataatgcc caaagctctc ggtcacatcc ctgagttgaa tggaccttgc
      301 cccactgaga gactgctcga tcagccggag ggtcgcgccc accaacaagg cgttgacgat
      361 gccgagtatg aacccgcctc ctattccgaa cggagcggcg atccgtgacg ttcccaaaaa
      421 cagaatggcg aatacgatga gagccaccac ggtcacccag cttcgcgcga acgaacgccc
      481 cgtcgcatac aggacatgcc gatacagttg aagcgtggct gaaacgaggt tgaccatgcc
      541 ggtgcgtacc ctaatcggga ttgagcaaga ggtcaaggac tagtcacacc cggaaacgaa
      601 aaaacggatt ctatggattc ttggttgact tctcgcgacc cgatgattat cagatggctc
      661 atcaacatat cttttcacgc gtatcttttt acaggtgagt caccatggac gtgaaccgaa
      721 tgacgatcaa attgcaagag gcgcttcagt ccgcctccgc ccatgcgcag cgaagaagcc
      781 atcagggcat cgatgtcgag cacatgttgt tggcgctgat cgaccaggaa ggtggaacgg
      841 ctccggctct gcttgagggg gccggtcttg ccctgcctgc ggttcggcag gcggtcgaac
      901 aagccttggc aaagctgccg caagtgcaag gctccggcgc cgctcccggt caaatgcaca
      961 ttgccacccg cctgactcag gtcctcaatc atgcggagga cgaacagaaa tcgctcaagg
     1021 acgattttct cagtgtcgag catgtgttgt tggccatggt tcaggaagga ggggtgctca
     1081 agaaactggg actcaccagg gaccgcctct tgtccggatt gcaacaggtg cgcggcaatc
     1141 agcgcgtcac cagtcaggat cctgaaagta cctaccagtc gctggtgaaa tacgggcgtg
     1201 atctgactca attggccggg caaggcaagc tcgaccccgt cattggtcgc gacgatgaaa
     1261 tcaggcgggt gattcaaatc ctgtcccgcc ggaccaagaa caacccggtg ctcatcggcg
     1321 aaccgggagt cggaaaaacc gccatcgtgg aagggctggc gatccgtatc gtcaaagggg
     1381 atgtcccgga gagtctcaaa cacaagaagc tcttcgcgtt ggacatgggc tcgctcgtcg
     1441 ccggggccaa gtttcgcggc gagttcgaag agcggctcaa agccgtgctc aaggagattc
     1501 aatcttccca aggccagatt ctgctgttca tcgacgagtt gcatacggtc gtcggtgcag
     1561 gcgccgctga aggcgcgatg gatgcggcca atctgttgaa gccgatgctg gcgagaggtg
     1621 aattgcatct catcggcgcc acgacgctcg acgaatatcg gaagcacatc gaaaaagacg
     1681 ccgccttgga acgccgcttt cagacggtcc tcgtcgacca gccgtccgtg gagaacacca
     1741 tttctatttt gcgcggcctc aaagagcgat atgaagtcca tcatggcgtg cggatcaagg
     1801 acagcgcgct ggtggcggcg gcgaaattgt cgcatcggta catttccgat cggtttcttc
     1861 cggataaagc catcgacttg gtcgatgaag ccgccgcgcg gctcaggacc gaaatcgaca
     1921 gccttccggc cgagctggat gaggtctcac gcaaggtgct ccagctggaa atcgagcggg
     1981 aagcattgag gaaagagaag gatccggcga gcgccgcccg cttgaccgct ctcgaaacgg
     2041 agctgaatga aaaacaacgc gacctgcaga ctcttaaaac cagatgggaa tcggaaaaga
     2101 cttcggtctc ccgcctccgc aaaacccgtg aggcgatcga agacgtcaaa ctgaagatcg
     2161 atcaagccga gcgggcctat gacctcaatc gggtcgccga gctgcgatac ggagaactgc
     2221 ctcgcctcga gcgggaattg gaactggaac aacagcattt ggggaaaaaa caggacgaga
     2281 caaaattgct gaaggaagag gtcgatgaag acgagatcgc cgcggtggtc agccggtgga
     2341 ccggtattcc cgtctctcga ttgctcgaag gggaatccga caaattgttg aaactcgagg
     2401 acctg
//
LOCUS       DATMTI010000750         1893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_664742, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000750 DATMTI010000000
VERSION     DATMTI010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664742"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VN039_11945"
     CDS             complement(<1..392)
                     /locus_tag="VN039_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46708.1"
                     /translation="MQRLPEIKATYDWFFAHYRAETAERAKINDIAGIDQMDVKRDIL
                     ERGIFVLMFGQFEKAVTSAFQDALNTRATSVDWTRRRGWDASSLKGKKVPFETKLAMV
                     LDSHSPSFAQIIQTYGTRNHCAHGGTTNA"
     gene            complement(394..837)
                     /locus_tag="VN039_11950"
     CDS             complement(394..837)
                     /locus_tag="VN039_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46709.1"
                     /translation="MNDDLDDMEERLQSPVTKDIVEARVEDWKNRLRALFRQVTAWAS
                     ENGWHVDDSGTVGMHEEIMQKFGVPATEQPTLRLDGEQGYVLFKPKGLWVIGANGRID
                     LYTSKGTFIIVDLAETGCPPTWTIFRATQKRDGDQFTPEMLASLV"
     gene            complement(1264..>1893)
                     /locus_tag="VN039_11955"
     CDS             complement(1264..>1893)
                     /locus_tag="VN039_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46710.1"
                     /translation="LTPVINQPDKAETWLEAFQLIATPTPGFKLTSGASYMGYNVEPL
                     LTAIAKLKPSDEACEVIWLRPSGSTWGRTQRVAEALVKIGDALRNAGRTNVGTVLAMP
                     RSTSKGVRTNHKRLFGRGMLLPQGHLPANLEVLIVAGVIAVVNVHLPVGGHSVPIGSV
                     VSDPRRLARMVERLTSRKGEGWEELWRAVAMKPSDEDSDSESYRTLDSA"
BASE COUNT          397 a          526 c          488 g          482 t
ORIGIN      
        1 gcatttgtcg tcccgccgtg ggcgcaatgg ttgcgtgttc cataggtttg tatgatctgt
       61 gcgaatgatg gcgaatgact atccagaacc attgccagct tcgtctcgaa aggcaccttt
      121 ttgcctttca gtgaggacgc gtcccagccg cgccgacggg tccaatcaac actcgtggct
      181 ctcgtgttca aggcgtcttg aaaagccgat gttacagctt tctcgaactg accgaacatc
      241 agaacgaaaa tgccgcgctc aagaatatct cgcttcacat ccatctgatc gatgccggca
      301 atatcgttga ttttcgctcg ctctgcagtc tccgccctgt aatgtgcgaa gaaccagtcg
      361 tacgttgctt ttatttccgg aagacgttgc atgtcagacg aggctcgcga gcatttctgg
      421 ggtgaattga tctccatcgc gcttttgagt agccctaaag attgtccaag tgggagggca
      481 gccggtttca gctaagtcga caatgatgaa cgtgcccttt gacgtgtaaa ggtcgatacg
      541 tccgttcgcg ccgatcaccc ataggccttt cggtttgaac agaacgtagc cttgctcacc
      601 gtctaaccgg agcgtcggct gctcggtcgc tggcacaccg aatttctgca tgatttcctc
      661 gtgcatcccg acggtcccgc tgtcgtctac gtgccaaccg ttctcggaag cccaggcagt
      721 gacctgtcga aaaagagcac gaaggcggtt cttccagtcc tctacgcgag cttcgacgat
      781 atcttttgta acaggggact gcaggcgctc ttccatgtca tccagatcat cgttcacgac
      841 ggctcccttc gttccagcat ctcgggtgag ttgaaaacat cttgcttcca tcatctcact
      901 tatatcacga gcgttgagag tcctacccag aaattccaag cccgaatcgt ttgtgtgaat
      961 acccgtaagt tcaaaggcgg atttttggtc agtcaatagt cccgacgacg ggctcatatc
     1021 ggactgccca gtagcaatag aggtcatcgt caggcgcaga gttagcagca ggcgttgatg
     1081 agcggcacca ggcggcggct tgcttaccgt tctccgctaa ccatcgatcc tgtagttttt
     1141 agcttgccga cgcggactga gatcgacata aagcacgcat aaggaggctt ggtatcgccg
     1201 tcaatgtcag tgtttgacga tctcgtgtcg tatgaccttt ttgaaactct gggccgctgt
     1261 cattcaggcc gaatcaagag tccgatagct ttcactgtcg ctatcctcgt cgctcggttt
     1321 cattgctact gcacgccata gttcctccca accttcacct ttgcgcgatg tcaggcgttc
     1381 caccattctg gcaagccttc tcggatccga gacaacgctg ccaatcggaa cggaatggcc
     1441 gccaactgga agatgcacgt tcaccaccgc gatcacgcca gctacgatga gaacttcaag
     1501 attggcgggc aggtgtcctt gcggcagcaa catgccacga ccaaacaacc gcttgtggtt
     1561 ggttcgaact cccttgcttg ttgaccgagg catagcgagc acggttccga cgttggttct
     1621 gcccgcattt cgaagggcgt ctccaatttt aacaagtgcc tccgcaactc tctgtgtccg
     1681 tccccacgtt gacccgcttg gacgtaacca aattacctcg caagcttcgt cggatggctt
     1741 gagtttcgca atcgcagtaa gaagtggctc gacgttgtag cccatgtagg atgcgcccga
     1801 cgtgagcttg aaccctggtg ttggtgtagc gattagctga aatgcttcca gccatgtttc
     1861 tgccttgtca ggttgattga ttacgggagt gag
//
LOCUS       DATMTI010000751         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_452694, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000751 DATMTI010000000
VERSION     DATMTI010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452694"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..125
                     /locus_tag="VN039_11960"
     tRNA            53..125
                     /locus_tag="VN039_11960"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:86..88,aa:Thr,seq:ggt)
     gene            183..1367
                     /gene="tuf"
                     /locus_tag="VN039_11965"
     CDS             183..1367
                     /gene="tuf"
                     /locus_tag="VN039_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWV46711.1"
                     /translation="MAKEAFKRDKPHVNVGTIGHVDHGKTTLTAAITKVLNKKGMAEV
                     REYDSIDNAPEEKERGITINTAHVEYSSDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPSIVVFLNKVDMVDDAELLDLVEMEVRDLLTQ
                     YEFPGDKIPIVRGSALKALNGDADSENQVLALVSAVDDYVPVPERPVDQPFLMPIEDV
                     FNIEGRGTVATGRVERGVLKKMEEVELVGIRPTAKTVATDIEMFRKLLDEARAGDNVG
                     VLLRGLKKEEVERGQVISKPGSITPHKKFKAEVYVLSKEEGGRHTPFFTNYRPQFYFR
                     TTDVTGTVKLPDGVEMVMPGDNISIEVELITPIAMEKTIRFAIREGGKTVGAGRVGDI
                     LD"
     gene            1470..1542
                     /locus_tag="VN039_11970"
     tRNA            1470..1542
                     /locus_tag="VN039_11970"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1503..1505,aa:Trp,seq:cca)
BASE COUNT          404 a          434 c          459 g          307 t
ORIGIN      
        1 ccggttcgcc gcccgggttt ccaagggagg cgctgggtgg tgacgagcag ccgcccatgt
       61 agctcagttg gcagagcacg tccttggtaa ggacgaggtc accggttcaa tcccggtcat
      121 gggctcggtg agcttgcgac cggtaacaaa aatccaaatt atagaggtaa acaaaaaacg
      181 atatggctaa ggaagctttc aaacgcgaca agccgcacgt gaacgtcggc acgatcggtc
      241 acgttgacca cgggaaaacg acgctcactg cagcaatcac gaaggtcctc aataaaaagg
      301 gcatggccga agtgcgcgaa tacgattcga tcgacaatgc gccagaagaa aaagagcgtg
      361 gaatcaccat caacacggcg cacgtagagt attccagcga caaacgccac tatgctcacg
      421 tcgattgccc cggtcacgct gactacgtga agaacatgat caccggcgcg gctcaaatgg
      481 acggcgctat tctcgttgtc tctgctgcgg acggccccat gccgcagacg cgtgagcaca
      541 tcctgctcgc caggcaggtc ggtgttcctt ccatcgtcgt tttcctcaac aaggtcgaca
      601 tggtcgatga cgccgagctc cttgatctcg tcgaaatgga agtgcgcgat ctgttgacac
      661 aatacgaatt tccaggagac aaaatcccaa tcgtcagagg cagcgcattg aaggcgctta
      721 acggcgacgc ggactccgag aatcaagtcc tggcgctggt atcggcggtc gatgactacg
      781 tgcccgttcc cgagcggccg gttgatcagc cgttcctgat gccgatcgaa gacgtgttca
      841 acattgaagg ccgcggcacc gtcgccaccg gtcgtgtcga gagaggcgtc ctgaaaaaga
      901 tggaagaagt tgagctggtc ggcattcgcc caacggcgaa aaccgtcgcg acagatatcg
      961 agatgttccg caaacttctc gatgaagcgc gcgcgggcga caacgttggc gtgctgttgc
     1021 gcggtctcaa gaaggaggaa gtcgagcgcg gccaggttat ttccaagccc ggctccataa
     1081 cgccgcacaa gaaattcaag gcggaagttt acgtgctgag caaagaggaa ggcggacgtc
     1141 acactccatt cttcaccaac taccggccgc agttttattt ccgcacaacg gatgtgactg
     1201 gcacggtgaa actccccgat ggcgtggaaa tggtgatgcc cggcgacaac atttcgatcg
     1261 aggtcgagct gatcacgccg atcgccatgg agaaaaccat tcggttcgca attcgcgaag
     1321 gcggtaaaac cgtcggcgcg ggtcgcgtgg gcgatattct ggattaaaat atcgcaacgg
     1381 tcatgtcgag cgacagtcga gacatcccgt tgcaaaaatg agaggtaccg caacgggatt
     1441 cttcgacttc gctcagaatg acacaactta cgccagtagc tcaattggta gagtagcggt
     1501 ctccaaaacc gtcggttggg ggttcgagtc cctcctggcg tgtggctaac gaaaatcggt
     1561 tacaacagtt acaaccttag attcaaaaca ataccgccgg attc
//
LOCUS       DATMTI010000752         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_282521, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000752 DATMTI010000000
VERSION     DATMTI010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282521"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VN039_11975"
     CDS             complement(<1..691)
                     /locus_tag="VN039_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46712.1"
                     /translation="MTKSPRALIILSMGLLVLGLIGVAPVSSETQEKEAMPEKPAPRA
                     QHPPAASDGQPTPHKPSGEAKPQSQPSPPTKADSSADHKEVTPEKVVPRLQQAPAASD
                     SQPKPSTETKPQSQPNPATKADSSTDHKEATPEKPAPRAPHPPAASEGQLVPSKPATE
                     AKPQSQPPPTIKTDVPPAEPESKEKDSVVKKPLGSLILSVKLALMGDTRLFRYDIEVG
                     DDQQTVTLTGRV"
     gene            complement(858..2177)
                     /locus_tag="VN039_11980"
     CDS             complement(858..2177)
                     /locus_tag="VN039_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV46713.1"
                     /translation="MSDALADSKETGSAPSRARGWELVKTRDFGFLFAGQTISQIGDS
                     LNKVALLWFVYEMTGSALKMTVVGLLQTLPPLLLGPLIGVYLDRVRKKPVMIGVDLLR
                     TLMVLLIPLLYAMGGLTLDRLYFLVFATAIFSTVFGPALTSAVPLIVPKERLIAANAL
                     LQTTTNVGLLVGPAVSGLGIALIGAQNVLYADAATFFISALCLFPIHMHETLDHWRTA
                     KKGLTEGITTDLLAGFRFVFVEQKTVLLLMLTATLYSVGISAFIFLLPVFAKDVLGVG
                     PIQLGWLWSALGVGMLLASLGLTSITQGDVSWRLRFMSGALAVGGLAVAALGFLEAPV
                     VAGALIAVIGGSTAMFTPMVWTMLQELTPAHLLGRVFTSFATGGMASSMAGMAGFGWA
                     ADTIGPAASLGGISLILLMTAATAWLFSFYKSHTGSLAISTSSPLAS"
     gene            complement(2170..>2966)
                     /locus_tag="VN039_11985"
     CDS             complement(2170..>2966)
                     /locus_tag="VN039_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWV46714.1"
                     /translation="LARRNTIPVVTTLHGRQDLPELEPVFREFSEMPLVSISDAQRKP
                     LPWANWAATVHHGLPRNLYTFHPQAQGYLAFLGRIAPEKRPDQAIEIAKRAGLPLRIA
                     AKVDPADQNYYEAEVEPLLNHPLIEFIGEISDAEKDEFIGNATALVCPYDWPEPFGLV
                     LIEALACGTPVLAYRRGSIPEIIDHGVTGFICETVAEMVDAVGQVPLIDRRRCRAAFD
                     ERFTADRMARDYVALYERLLLEDEAVQATPAFASEQHKILGRGRHV"
BASE COUNT          661 a          842 c          867 g          596 t
ORIGIN      
        1 agacacgtcc tgtcagggtc acggtttgct ggtcgtctcc cacttcaata tcatagcgga
       61 aaagccgtgt atcccccatc aacgcaagct tgaccgacag aatcagtgaa cccagcggct
      121 tcttgacaac ggaatccttc tccttgcttt caggttcagc cggtggcacg tcggttttta
      181 tcgtcggagg tggttgggac tggggcttcg cttccgtcgc cggtttggaa ggcacgagct
      241 gaccctcgga agcagccggg gggtgtggcg cgcgtggtgc cggcttctcc ggcgtagctt
      301 ctttgtgatc ggttgacgaa tccgcttttg ttgcaggatt gggttgagac tgcggctttg
      361 tttccgtcga gggtttgggt tgactgtcgg aagcggccgg agcctgttga agacgtggca
      421 caaccttctc cggcgtaact tctttatgat ccgctgacga gtccgccttc gtcggaggac
      481 tgggttgaga ttgtggcttc gcttctcctg atggcttgtg cggcgtaggc tgaccgtcgg
      541 aagcggccgg aggatgttgc gcgcgtggtg ccggcttctc cggcatggct tccttttctt
      601 gtgtctccga ggacactggg gccacaccta ttagccccag caccaacagg cccatactca
      661 gtatgatcag cgctcgtggc gacttggtca tcgtgggagc ccctcctcgt gacgatattc
      721 ctgagaattc atccagccat tctaactcgc gacggctttc gtgtcatcta ttcccggagc
      781 gagacggccc ccttgtgtct cagagggccg ttccggggtg atgcgtctgt tgatggaaac
      841 ttcgaggcgg tggcgggtta agaagccaag gggctggacg tagaaatggc caagctccct
      901 gtgtgcgact tgtaaaagct gaacagccat gcggtcgcag ccgtcatgag aagaatgagg
      961 ctgatgcctc ccaggctggc ggccggaccg atggtatcgg cagcccatcc aaaacccgcc
     1021 ataccggcca tggatgacgc cattcctccc gtggcgaagc tcgtaaagac tcggcccagg
     1081 agatgcgccg gcgtcaattc ttggagcatc gtccacacca tcggagtaaa catagcggtg
     1141 cttcctccga tcactgcgat cagggcaccg gccacaacag gagcctctag gaaaccgagc
     1201 gccgcgaccg caagaccgcc gacggctaag gcaccggaca taaaccgcaa ccgccaagat
     1261 acgtctccct gcgtgattga tgtcagccca agggaggcaa gcaacattcc aacgccgagg
     1321 gcggaccata gccaccccag ctgaatcggg cccaccccca gcacgtcctt agcgaagacc
     1381 ggcagcaaaa agatgaaggc actgatgcca acgctgtaga gagtcgccgt caacatgaga
     1441 agcaagaccg ttttctgctc gacgaagacg aaacgaaaac ccgccagcaa gtccgttgtg
     1501 atgccttccg tcaatccttt cttcgccgta cgccagtggt cgagagtttc atgcatatga
     1561 atggggaata aacacagtgc ggaaataaaa aaggtcgccg cgtcggcata gagcacgttt
     1621 tgtgctccga tgagcgcgat accgagaccg ctgaccgcag gccccacaag aagtccgacg
     1681 ttggtcgtcg tttgcagtaa cgcattggcg gcgatgagcc gctctttggg cacaatgaga
     1741 ggaaccgcag aggtcagggc cggcccgaag accgtggaga agattgcggt ggcgaacaca
     1801 aggaagtaga gccggtcgag agtcaggccc cccatcgcgt agagcagtgg gatcaggaga
     1861 accatcaggg ttcggagaag atccactccg atcatgaccg gtttctttcg aacgcgatcc
     1921 agatatacac caatcagcgg ccccaacagc aagggcggca gggtttgaag tagtccgacc
     1981 accgtcatct tgagcgccga cccggtcatc tcatacacaa accagagcag cgccaccttg
     2041 ttgaggctgt cgccgatctg agagatcgtt tggccggcga acaaaaagcc gaagtctcgg
     2101 gttttaacta actcccagcc gcgagcacgg ctcggagcgg aaccggtctc ttttgaatca
     2161 gcaagtgcgt cagacatgcc ggcctcgccc aagaatctta tgctgctctg aggcgaaggc
     2221 gggtgtggct tgaacagctt catcttcgag aagaagacgt tcatacagcg ccacatagtc
     2281 gcgcgccatt cgatccgcag taaaccgctc gtcgaaggca gcgcggcagc gccgccgatc
     2341 gatgagggga acctgtccga ctgcatccac catttcggct acagtctcgc aaataaagcc
     2401 ggttacgccg tgatcgatga tttcaggaat cgatccacgt cggtaggcta acaccggagt
     2461 tccgcaagcc agggcttcga tcaataccaa tccgaaaggc tccggccaat cgtaaggaca
     2521 caccaatgcc gtcgcattgc cgataaactc atctttctca gcatcggaga tctctccgat
     2581 gaactcaatc aagggatggt tgagcaacgg ctcaacttcg gcttcatagt agttctggtc
     2641 ggcaggatcg acttttgccg caatccgcag aggaaggccg gcgcgtttag cgatttcaat
     2701 cgcttggtcc ggacgttttt caggggcgat gcgccccaga aaggccagat acccttgggc
     2761 ctgaggatga aacgtgtaga ggttgcgagg aagaccatga tggacagtcg ccgcccagtt
     2821 ggcccaaggc agcggtttac gctgggcatc cgaaatggac accaaaggca tttcagaaaa
     2881 ttcgcgaaaa accggctcca attccggaag atcctgacga ccgtgcagag tcgtcactac
     2941 cggaatggta ttcctccgtg ccagcg
//
LOCUS       DATMTI010000753         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_282532, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000753 DATMTI010000000
VERSION     DATMTI010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282532"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /gene="tssC"
                     /locus_tag="VN039_11990"
     CDS             <1..1451
                     /gene="tssC"
                     /locus_tag="VN039_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HWV46715.1"
                     /translation="TLEQESAGLLDQIITKTKIGRDTEQKTQSRQQIATFVEEVLKGS
                     IKRSKDVETTISSRIAEIDALLSKQLNEILHAPELQKLEASWRGLRYLVSRSETGSTL
                     KIRVLNCTKKELLRNFGNASDFDQSVLFKKVYEEEYGTFGGEPIGALIGDFEFNRTAQ
                     DMALLEQMSHVAAAAHAPFLTAADPQLFNWDEFTEMAGPRDLSKVFDTVEYAKWKSFR
                     DSEDARYVGIGLPHILMRLPYGPDTSPVETFHFAEDVDGKDHKKYLWGNAAYALGTRL
                     TTAFAKYGWCAAIRGVENGGLVEGLPTHTFHTDQGDVALKCPTELAITDRREKELADL
                     GFIPLVHCKGTDYAAFFSVQSAQKVKKYDTDAANANARLSAQLPYLMAVSRFAHYLKV
                     MMRDKIGSFVSRSECERFLNQWISNYVTPDDNATAATKAQYPLRDAKIEVVEAPGKPG
                     VYKAVAFLRPHFQLDELSVSLRLVAELPKSAK"
     gene            1496..1984
                     /locus_tag="VN039_11995"
     CDS             1496..1984
                     /locus_tag="VN039_11995"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HWV46716.1"
                     /translation="MAVADYFLKIEGIDGESTDDKHKGEIDVLSWSWGETQSGTHHAG
                     GGGGAGKVSMQDFHFVMKVNKASPKLMQACASGEHIKKGVLVCRKAGKDQQEYLKITL
                     SDMLVSSYQTGGSSGAEIIPTDQISLNYAKIEFEYKEQKQDGTLGGAVKGGWNVKENK
                     AA"
     gene            2055..>2472
                     /gene="tssE"
                     /locus_tag="VN039_12000"
     CDS             2055..>2472
                     /gene="tssE"
                     /locus_tag="VN039_12000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HWV46717.1"
                     /translation="MARGNTEPTVVASLLDRLTDEHPVSMREAVPLRLHDVNQLRISV
                     ARDLEALLNTRREYLDELPEHYSEMERSLLLYGLPDFTSYSLKSQRDRHRVLQAVEEA
                     IVRFEPRLQDVHVQIIPEEALGKNLHFRVEGMLKVEP"
BASE COUNT          583 a          632 c          763 g          494 t
ORIGIN      
        1 ccacgctgga acaggaaagc gccggtttgc tcgaccagat catcaccaag accaaaatcg
       61 gtcgtgacac cgagcagaaa acccagtccc gtcagcagat cgccaccttc gtcgaagaag
      121 tgctgaaggg gtcgatcaaa cgctccaagg acgtcgagac gacgatcagc tcacgtatcg
      181 ccgaaatcga cgccctgttg tccaagcaac tcaacgaaat tctgcacgcg ccggaattgc
      241 agaagttgga agcatcatgg agaggtctcc gctatctcgt ctcacggagc gagaccggtt
      301 cgacgttgaa gataagagtc ttgaactgca cgaagaaaga gttgctccgg aactttggaa
      361 acgcatccga tttcgatcag agcgtgctgt tcaagaaagt gtacgaggaa gagtacggca
      421 cgttcggcgg cgagccgata ggggcgttga tcggggattt tgaattcaat cgcaccgctc
      481 aggacatggc tctgttggag cagatgtccc atgtcgccgc ggcggctcat gcccctttcc
      541 tgacggcggc ggatccccaa ttgttcaatt gggacgagtt caccgaaatg gcggggccgc
      601 gcgatctttc gaaggtcttc gatacggtgg aatacgcaaa atggaaatcg ttccgggact
      661 cggaggatgc gcgatacgtg gggatcgggt tgccgcatat cttgatgcgt ctgccgtatg
      721 gtccggacac gtctccggtg gagaccttcc atttcgcgga ggatgtcgac ggcaaggatc
      781 ataagaaata tctctggggt aatgccgcct acgcgctcgg tacccgtctg accaccgcgt
      841 ttgcgaaata cgggtggtgc gcggccatcc gaggagtgga aaacggcgga ttggtagaag
      901 gcttgccgac gcataccttc cataccgatc aaggcgatgt ggccttgaaa tgtccgacgg
      961 aactggccat caccgacagg agggaaaagg agcttgccga cctcgggttt attccgctcg
     1021 tgcactgtaa gggcaccgac tatgcggcct tcttcagcgt gcaatcggcg cagaaggtaa
     1081 aaaagtacga taccgatgcg gcgaacgcga atgccagatt gtccgcacaa ttgccgtatc
     1141 tcatggcggt gtcccgcttc gcgcactatt tgaaagtgat gatgcgggac aagatcggaa
     1201 gctttgtgtc caggtcggag tgcgaacggt ttctcaacca atggatctcg aattatgtga
     1261 cgccggacga taatgcgacc gcggccacca aggcgcaata ccccttgcgc gatgccaaga
     1321 tcgaagtggt tgaggcgcct gggaagcccg gagtgtacaa ggccgtcgcg tttctgcgac
     1381 cgcatttcca gttggacgag ttgtcggtgt ccttgcgtct cgtggcggaa ttaccgaagt
     1441 cggcgaaata agcggcatca atttcatcaa cctcatgtga ggaaggagga cgcgaatggc
     1501 agtcgcagac tattttctaa agatagaagg cattgacggt gaaagcacgg atgacaaaca
     1561 taagggggaa atcgacgtgc tgtcttggtc ctggggagaa acgcagtccg gcacccatca
     1621 tgccggcggc ggcggcgggg cgggaaaggt ctccatgcag gactttcatt ttgtcatgaa
     1681 agtcaataag gcatcgccga agttgatgca ggcctgcgcc agcggcgaac atatcaagaa
     1741 gggggtgctg gtgtgccgaa aggccggaaa agaccagcag gaatatctca agattaccct
     1801 gtccgacatg ctcgtttcgt cctatcaaac gggcggatcc tcgggggcgg agattattcc
     1861 gactgaccag atctcgctca actacgcaaa gatcgagttc gaatataagg aacagaagca
     1921 ggacggcacg ttgggcgggg ccgtcaaggg cggctggaac gtcaaggaaa acaaggccgc
     1981 gtaatcgccg gtcgtgatgc aatggcccgg ttcgcccggg ccatcatggc gaactgtaag
     2041 cggaggactc gctcatggcg cgtggaaaca ccgaaccgac cgtcgtggcc tcgttgctcg
     2101 accggctgac ggacgagcat cccgtctcga tgcgagaggc tgtgccgctc aggctgcacg
     2161 atgtgaatca attgcgtatc tcagtggcga gagatctcga agcgctgctc aatacccggc
     2221 gcgaatatct cgatgaattg ccggaacatt actccgagat ggagcgctca ttgctgttgt
     2281 atgggctccc ggatttcacg tcgtacagtc tcaagagtca gcgtgaccgt caccgtgtcc
     2341 tccaggcggt cgaagaggcg atcgtgaggt ttgagccgcg tctgcaggac gtgcacgttc
     2401 agattattcc ggaggaagcc cttggaaaga atttgcattt tcgagtggag ggcatgctga
     2461 aagtggagcc gg
//
LOCUS       DATMTI010000754         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_112793, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000754 DATMTI010000000
VERSION     DATMTI010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112793"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            637..1173
                     /locus_tag="VN039_12005"
     CDS             637..1173
                     /locus_tag="VN039_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46718.1"
                     /translation="MTEVAGKFVTTELMAHIRAQFRLDWHGVHGASHWGRVMHHGLAL
                     AELTGADTQIVRLFAVLHDSQRHDDYADPEHGVRAASYAYALHKDGLIHLSSDGLDVL
                     MTACRGHSYGHVDADITIQTCWDSDRLDLGRVGIRPAPRYLCTSAAKDPERIERAWRW
                     SRDEHSFEVPRNPLGIHW"
     gene            complement(1164..1736)
                     /locus_tag="VN039_12010"
     CDS             complement(1164..1736)
                     /locus_tag="VN039_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46719.1"
                     /translation="MLASARCFFVIELQTDQGIVQVSTPFKIILGILAALVGLIVYNG
                     IKAQSEDADSSGLSTNSQTAALAESSRAIGNAGNASSNSTSELPSSESPVDVQVRVLT
                     ENLDLDPVGHTTSLTVHPNRIYIVAKIDRVTIKNVVINRGNCSIVDYKNRLPAIMRYG
                     QTWEIYTSSGCNVIEVSVDTDHGTWTANYQ"
     gene            complement(1758..1955)
                     /locus_tag="VN039_12015"
     CDS             complement(1758..1955)
                     /locus_tag="VN039_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46720.1"
                     /translation="MAGISGGVIVRDDGRQIQFRPKCERCGNVGPTIYNIAPPTQGSN
                     VSGWQFRCDRCGSDTTITVYG"
     gene            complement(1977..2195)
                     /locus_tag="VN039_12020"
     CDS             complement(1977..2195)
                     /locus_tag="VN039_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46721.1"
                     /translation="MDYYLIPEQVVGRALLGGGGLTLMQSRQLDAYRALREFCRQLCE
                     QPDFFFNDDTRDRLVELLVICSLADTGI"
     gene            2255..2533
                     /locus_tag="VN039_12025"
     CDS             2255..2533
                     /locus_tag="VN039_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV46722.1"
                     /translation="MSTHHPHYQLLTKLLREVREQRGLSQVELAERLGNTQTFVSKVE
                     RGERRLDVVELIEFCEAMEVPVDQWLKDYLKRRTSELKNHPAKRKTAQ"
     gene            complement(2656..>3221)
                     /locus_tag="VN039_12030"
     CDS             complement(2656..>3221)
                     /locus_tag="VN039_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46723.1"
                     /translation="IINSNTCNRTNPRIYSFSDSMRVDNAYEILRRGPYVFVAQVVYD
                     SHASPGVPRIAPSTLVDQVSNYVRYMHEVMREMKVYGSDVPLGGIAMIDTARRKGGQL
                     VVQLGFNDHRVFRVPADVAHERFTKMVVEGWDRNVRDSANPSLGAALHPRKNHGTAET
                     PRENTQYLVDSATTRQHLLHIHRDGVC"
BASE COUNT          734 a          862 c          860 g          765 t
ORIGIN      
        1 gtcaaggaag gagcgctttt cgcaagtgaa gcctcgatcg tacagacacg attgacgacc
       61 catgctcttt tgccaaacca gttagtagag cgcatgccga aggtaggatg agtggacaaa
      121 atcagcgcgt cgatgcgaag ggtagaagat gagccaagcg agtcggctgt attaggaccg
      181 cttcttcggg cttcttttcg ctggagctct aggagcaagc gctaatactt cagcggcctc
      241 cttgcgttct cctttgttct tcgaatgcat atccttaccg gcacgcgaga ggttcgcagg
      301 agagggggtc cgtggcttgt ttgccatatc tatgctccaa aaggacgttg gtcaaaccct
      361 tcttacccct ctgaccggtt ctttgcaatc acaccctcgg taaagcgcac cagaaggctg
      421 cgaaagtcat cttggacaac tgagtagcac tcgcccgtcg aaagcctgta catgccttcc
      481 cagaagcgtt tgaaaggcag tcgacgaaaa gaccaaactt ggattggcga tacaggaatt
      541 tcagattgcc gtcgcgacgg tttggtgaac aaactctatc ggagcatgtg ccactactga
      601 cggagccacc cgtcgatgca ttaaaattgt acttcgatga ctgaggtggc cggcaaattc
      661 gttacaactg agctgatggc tcacatccgg gcgcagttcc gacttgattg gcatggcgtt
      721 cacggtgcat ctcattgggg ccgcgtgatg catcacggct tggcacttgc cgaactcact
      781 ggcgctgata cacaaattgt tcgattgttc gctgtgctgc atgattcaca gcgccacgat
      841 gactacgccg accctgagca tggtgttcgc gctgcgagct atgcatatgc attgcacaag
      901 gacggactga tccacttatc aagcgatggt ctagacgtcc tgatgactgc ttgccgtggc
      961 cacagctatg ggcacgtgga tgctgatatc accatccaga cttgctggga ttccgacagg
     1021 cttgatcttg ggcgtgttgg catccgtcca gccccccgtt acctctgtac atcagccgcc
     1081 aaggaccccg aacgcatcga gcgtgcttgg aggtggtcac gtgatgagca ttcgttcgaa
     1141 gtacctagaa accccttggg aattcactgg tagttggctg tccacgtgcc atgatccgta
     1201 tccaccgata cctcgatcac attgcatcca gaacttgtgt agatctccca agtctggccg
     1261 tacctcatga tcgcgggcaa ccgatttttg tagtctacga tcgagcaatt acctcgattg
     1321 atgaccacat tcttgatggt caccctatca atcttcgcaa caatgtagat gcgatttggg
     1381 tgaacggtta gcgatgttgt gtggccgacg gggtccaaat caagattttc cgtcaaaact
     1441 ctaacttgta catccaccgg agactccgat gatggcagtt cagaagttga atttgacgaa
     1501 gcgtttcccg cattcccgat agcccttgag gattctgcta gggctgctgt ctgcgaattc
     1561 gtggacaggc ctgagctgtc tgcatcttca ctctgggcct tgatcccgtt gtatacgatc
     1621 aagccaacga gtgcggccaa gatgcccaga atgattttga aaggtgtact cacctgcacg
     1681 atcccctgat cggtttgaag ttcgatgacg aagaagcaac gtgccgaagc aagcaaagtt
     1741 cgtctggctt ggcgtgccta accgtaaacg gtgatggtcg tgtcagagcc gcaccgatca
     1801 caacgaaatt gccaacccga gacattggac ccttgggtcg gtggagcgat gttgtagatg
     1861 gttggaccga cgttgccgca acgctcgcat ttcggacgaa attgaatctg acgtccgtca
     1921 tcccgcacga tcactccacc agaaatgcct gccatgtgaa ccctccaagt tgtggtctaa
     1981 atgccggtat cggccaacga gcagatgaca agcagctcca ccaagcggtc tcgcgtgtca
     2041 tcgttgaaga agaagtccgg ttgttcgcac agctgcctgc agaactcccg cagtgcccga
     2101 tacgcatcga gctgtcgcga ctgcatcaaa gttagccctc ctcctccaag cagcgctctg
     2161 ccgaccacct gctcagggat caaataatag tccatattgg actattccga aacagacttt
     2221 agcgttggcg tacctcggtg agcaagtctc gtctgtgtct acccatcatc cgcactatca
     2281 gctgctgacg aagcttctaa gggaagtcag ggagcagcgt ggactctcgc aagttgagct
     2341 ggccgagcgg cttgggaaca ctcagacctt cgtgtccaag gtcgagcggg gtgaacgacg
     2401 actggatgtt gtggagctca tcgaattctg cgaggcgatg gaagttccgg tcgatcaatg
     2461 gctcaaggac tacttgaagc gacgaacgtc ggaactgaaa aatcatcctg cgaagcgcaa
     2521 gactgcacag tagtcgttgc ctgctggcag tgacgcgtcc aatgaacttg agtgccttgg
     2581 tatgcccgga tctggtcagg tagcaaccta aaggcccacc gaccgggcca gattccggtc
     2641 ggtgcttgag cgcatttaac agacgccatc tcgatggata tgcagcagat gctgacgcgt
     2701 agtcgcacta tcgacgaggt actgcgtgtt ttctcgcggc gtttctgcgg tgccgtggtt
     2761 cttccttggg tgcagagccg cgcctagcga agggttggct gaatcacgaa catttctgtc
     2821 ccagccttcg acgaccatct ttgtaaatct ttcgtgtgcc acatccgccg gcactcggaa
     2881 aacgcgatga tcgttgaagc ccaattgcac aacgagctga ccacctttgc gtcgcgcggt
     2941 atctatcata gcgatgccac caagcgggac gtcgctaccg tagaccttca tctctcgcat
     3001 cacttcatgc atgtaccgca catagttgga cacttgatct acgagtgtac ttggagctat
     3061 tcgcggtacg ccggggctgg catgggagtc gtaaaccacc tgtgccacga agacgtatgg
     3121 acctcttcta agaatttcat acgcattatc cacgcgcatc gaatcagaaa aactgtagat
     3181 ccgtggattg gttctgttgc aggtgttgct attaataata g
//
LOCUS       DATMTI010000755         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_622237, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000755 DATMTI010000000
VERSION     DATMTI010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622237"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VN039_12035"
     CDS             complement(<1..390)
                     /locus_tag="VN039_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46724.1"
                     /translation="MTGYGKRVLIVEHDEGERNVLGLMLENEGYNVHVVSEEGPALEE
                     LKRRRFDVVVSAHHMPQINGFRLILMGRLVWPDTPIILLLGNETSLSETVEQGGAYGY
                     LRKPYVFGELLDLIESAIKLSRKHRLQT"
     gene            complement(527..1408)
                     /locus_tag="VN039_12040"
     CDS             complement(527..1408)
                     /locus_tag="VN039_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HWV46725.1"
                     /translation="MPHLAKAILLVTLVLFLQSCGTTVQPGQRGLRWYPLTAGLTTET
                     LKSGFYWRAPWNQVYLYDVQLRSYTETVDALSSDDLLVILKTAIIMRPIPEEVYFLAQ
                     EIGSDFYPRVVKPELLAAVRSVVSNYPMVNVPEKSSEIASKVQAVVVEKLKGRHLEVA
                     SVAMADIQLAKVVLDAVERKQAKEQEKEQKEFELIIAEKDAEIARRHARGEGDAIRIR
                     SEGEAEGLKIRATGQAKAQETITKTLTAEYLRFKLYDSSNSKFVLLPDKLNVPVIVNP
                     GTDQPTSLSHEDFRTRR"
     gene            complement(1448..1888)
                     /locus_tag="VN039_12045"
     CDS             complement(1448..1888)
                     /locus_tag="VN039_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46726.1"
                     /translation="MPIPVRKKNVLDEQHGRVRVGRMKRLSFTHFVLLLALGVSGMNG
                     CLASTTKEELDLTTYDPSQDHRKIAAYYSREATKLRQTSEEMSVRIAVYERLFGPESD
                     WVSGTRLLAQSYQDAAREHELKAREHLDLIAESRRPSAARSGRR"
BASE COUNT          483 a          645 c          551 g          572 t
ORIGIN      
        1 ggtttgtagt cgatgcttac ggcttaattt gatggcgctt tctattagat ccagcaactc
       61 accaaaaacg tagggtttgc gaaggtagcc atatgcccct ccttgctcga ccgtttccga
      121 cagacttgtc tcgttcccca atagcagaat aataggtgtg tccggccaaa caagccgtcc
      181 catcaagatg agacgaaatc cattgatttg aggcatgtga tgggcactga ccaccacatc
      241 gaatcgtcga cgtttcaatt cttccaatgc tgggccttct tcagacacca cgtgaacgtt
      301 atatccctca ttctccagca tcagacccaa cacattccgc tcgccttcgt cgtgctccac
      361 gatcagcact cttttcccat acccggtcat gtcgacctcc ttcgctcaat tctagaccga
      421 ctctttgccc tccgtttcac gaacccataa gagcaccacc ggcggatgat gactttcctt
      481 cgtccttttc aataatccat tgggtacgtg agcgcatgcc tgaatctcag cgtctggtac
      541 ggaagtcctc gtgtgacaaa gacgttggtt gatcagtgcc gggattgacg ataaccggca
      601 cattcaattt gtccggtagc agcacaaact tggagtttga gctgtcgtaa agcttgaacc
      661 ggagatattc cgccgtcagc gtcttggtaa tggtctcctg cgccttcgcc tgaccagtcg
      721 cccgaatttt caggccttct gcttcgcctt cggaccggat ccgaatcgca tcgccctcac
      781 ctctggcgtg cctgcgagca atctccgcgt ccttctcagc aatgatgagc tcgaattctt
      841 tttgttcttt ctcctgttct ttcgcttgtt tgcgttcgac ggcgtccagc acgaccttgg
      901 ccagttggat atcggccata gcaacgctcg ccacttcgag gtgacgcccc ttgagtttct
      961 cgaccacaac cgcctgcact ttgctggcaa tttccgagct tttttccggc acattgacca
     1021 tgggatagtt ggagacaaca cttctgaccg ctgccaacaa ttcaggcttg acgacgcgag
     1081 gatagaaatc tgatccgatc tcctgggcca agaaatagac ctcttcggga atcggcctca
     1141 tgatgatggc ggtcttgagg atgaccagca gatcatctga gcttagagca tcgacggttt
     1201 cggtgtaact gcgcaattgc acatcgtaca ggtagacttg gttccatggc gcccgccaat
     1261 agaatccgct cttgagtgtt tcggtggtca gacctgcagt caagggatac caacgtaacc
     1321 cccgttgacc gggttggacc gtggttccgc aggattggag gaagagtact agcgtgacaa
     1381 gaagtatggc tttcgcaaga tgaggcatag tctctccttt tcgcccgaat gttgactggc
     1441 gggcgggcta acgacgtccc gaccgtgcgg cggacggtcg tcgtgactcg gcgattaggt
     1501 caagatgttc tcgcgctttt agttcatgct ctctcgctgc gtcttgatag gattgcgcca
     1561 agagccgagt tcccgatacc caatccgatt caggaccaaa aagccgctca tacacggcga
     1621 ttcgaactga catctcttct gaggtctgtc gcagcttcgt cgcttcacgg ctataatagg
     1681 cggcaatctt ccggtgatct tgcgaggggt cgtaggtcgt taagtctagt tcctcttttg
     1741 tcgtcgacgc cagacagccg ttcatgccgg acactcccag tgccaacagc agtacaaagt
     1801 gagtgaaaga caaccgtttc atccgtccaa ctctcacccg cccatgttgc tcgtcgagca
     1861 cattcttttt gcggactgga atcggcaacc acctacagat cccgaacact aacgactctc
     1921 accatgtccc ttgatgggga ataaaaatgt atcgcgctaa tcccacgctc gattgattct
     1981 ctaagacata gttctcaggt tctgtcatca aatcgctgct gtgcccgtgt cgcatgatgc
     2041 cctcccgatc cggagctgcc tgccagtgcg agctttgagc cggccttgat ctgcgaagcc
     2101 accaacagtg gctagcagca aacccgatgc cactggaggg aaggcattac acttaccacc
     2161 gcttgggagt tcacggtgat tcccctcaaa tctggatata atgcgacgga gagatcctac
     2221 atgggctagt gacagtctgg cggcgttatg c
//
LOCUS       DATMTI010000756         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_664886, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000756 DATMTI010000000
VERSION     DATMTI010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664886"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VN039_12050"
     CDS             <1..662
                     /locus_tag="VN039_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46727.1"
                     /translation="KSDYTVPQREFTDKFMTSPWGLSTSSVPELARGIRKDSSSPAPG
                     ESEYEFVRNNLGGVRQYMVRRSCKYLLYDVVNRGGSVHYALDDVGLDKLAGDDGVPSP
                     VLLPRKNVHKRPVCTSEIRELFRMWNYFGKDFRVIFYKELEYTNAPWDITQYRRGWAA
                     YARHLATKAIAKLGPAIGADIQGKLTAVVNERADDAAIKKYHEINYVQLVAKGNGVIV
                     G"
     gene            684..893
                     /locus_tag="VN039_12055"
     CDS             684..893
                     /locus_tag="VN039_12055"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46728.1"
                     /translation="MLIPLEYDHEVVKLVVSRCTESESGGRMIDAILTNTMLPDVPRG
                     FLSRMIAGAPVKVGAGDAGFSYAFA"
     gene            987..2102
                     /locus_tag="VN039_12060"
     CDS             987..2102
                     /locus_tag="VN039_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46729.1"
                     /translation="MTVSYRNYQDSRINELVFAGTHDAGINKGSGNEKTQDLDIGGQA
                     AEGARIFDLRIAAKSTGVTGSGVLKAYHGQGLHKSIGGNRLVAGTWGGGLEGMLQDAR
                     RFVTTKSTEFLFFKFDHCKNWGKIAEMCVDVLGDAIYTGGGNLNMKTAGDLGGKVIVL
                     FTNAGLQEIGGARPGQGIVGIKSLYEKGENQKAYDPNYVGLQYHGKGGTSAMNGKSDD
                     KKINENVKKQTTIISTGRKINGGDPRVMGMIYWTSTGLFRNIKSRDNFNWAKGSFGDG
                     MVRLWLDGVGEYIDDNLPLYIMRDAYGSASMLKRFMPNFVMIDFADSQKCETVFSLNE
                     LKGTRLVQAAINYGAISPERYQDGLQKTMQRGWVRAV"
     gene            2299..3237
                     /locus_tag="VN039_12065"
     CDS             2299..3237
                     /locus_tag="VN039_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46730.1"
                     /translation="MELKIISWNIRHFREKKIFDFIHTLWEVIVWCDVCFIYEDHMGN
                     GTALAKALNEYESKLPLDKVRNHWKGEEIHATDEYVAIVWKSGVQVTPHSAATAVFSE
                     LMSGERCPAAVKIEKSEKSLIVAAWHAYGPAKVSAKDLFQKILQVNICDVLIGDFNFQ
                     TFATGLGSGGLNVTDDDFSLVESLDFKVPPMRTSPATSFGLSAVPSSSSAVPMAASSS
                     SFSSPDPVPGGKSPPMRSSRLEDKVHMKEIVPANHQGSTTYTEDRVGKRTTGLDRCLV
                     RKQFVSSCKLMVAMPEWFDELFCLTDHMVLQLIIDI"
BASE COUNT          738 a          856 c         1095 g          710 t
ORIGIN      
        1 ccaaaagcga ttacaccgtg ccgcaaagag aattcacgga taaattcatg accagtccgt
       61 gggggctgtc gacgtcttct gtgccagagc tggcccgagg cattcgcaag gattcatcct
      121 cgccagcgcc aggcgagtcg gagtacgagt ttgtaaggaa caacctgggt ggcgtcaggc
      181 agtacatggt gaggcgctcg tgcaagtatc tgctctatga cgtggtcaat cgtggtggtt
      241 ccgttcatta cgcgcttgat gatgttgggc tggacaagct ggccggcgac gatggcgtgc
      301 cgtcgccggt cttgctgcct agaaagaatg tgcacaagcg gcccgtctgc acctcagaga
      361 ttcgcgagtt gtttcgcatg tggaattact ttggcaagga ttttagggtc attttctaca
      421 aggagcttga gtacacgaac gcaccttggg atatcaccca gtacagaagg ggatgggctg
      481 cctatgcgag gcacttggcg accaaggcga tcgccaagct gggcccggcc ataggtgccg
      541 acattcaagg caagttgacg gcggtggtga atgaaagggc cgacgatgcg gccatcaaga
      601 agtaccacga gatcaactat gtgcagctgg tcgctaaggg caatggggtg atcgtgggat
      661 agggaaaagg tgccgggggc agtttgctca tcccgctcga gtatgaccac gaggtggtga
      721 agcttgtggt gtcgcggtgc acggagagcg agtcgggtgg gcgcatgatc gatgcaatcc
      781 ttaccaacac catgttgccc gatgttccgc gcggattcct cagtcgcatg atcgcgggtg
      841 caccggtgaa ggtcggggct ggcgacgcag gcttttcgta tgcctttgct tgagctttca
      901 ggggtctaac gaattgatcg taacggcgtg aactttcgtc gctttgccac tgcgccaggc
      961 aagaagaatt tttggaggac atggttttga ccgtctcata ccggaactac caagacagcc
     1021 gcatcaatga actggtgttt gccggcaccc acgatgcagg catcaacaag ggttcgggaa
     1081 acgaaaagac acaggacctg gacatcggcg gccaggcagc ggagggcgcc cgcatcttcg
     1141 acctgcgcat tgcggcgaaa tccaccggcg tcacgggttc gggcgtgttg aaggcctatc
     1201 acggccaggg cctgcacaag tccatcggcg gcaaccgctt ggtggccggc acctggggcg
     1261 gcgggctgga gggcatgctg caggacgcca ggcggttcgt gacgaccaag tccaccgaat
     1321 tccttttctt caagttcgac cattgcaaga actggggcaa gatcgccgag atgtgcgttg
     1381 acgtgctcgg ggacgcgatc tataccggcg gcggcaacct caacatgaaa acggccggcg
     1441 atctgggcgg caaggtgatc gtgctgttca ccaacgccgg cctgcaggag atcggcggcg
     1501 ccaggccggg gcagggaatc gtcggcatca agagcctgta cgagaaaggc gagaaccaga
     1561 aagcctacga cccgaattat gtcggcctgc agtaccacgg caagggtggc accagcgcca
     1621 tgaacggcaa gtccgacgac aagaagatca acgagaacgt caagaagcag acgaccatca
     1681 tcagcacggg acggaagatc aacggtggcg atccccgggt gatggggatg atctactgga
     1741 catcgaccgg cctgttccgg aacatcaagt cacgcgacaa cttcaactgg gccaagggca
     1801 gtttcggcga cggcatggtc aggctctggc tcgatggcgt gggcgaatac atcgacgaca
     1861 acctgccgct ctacatcatg cgggatgcct acgggtccgc cagcatgctg aagcggttca
     1921 tgccgaactt cgtcatgatc gatttcgctg acagccagaa gtgcgaaacc gtcttttccc
     1981 tcaacgaatt gaaggggacg cgattggtcc aggcggcgat caactacggc gcgatcagtc
     2041 cggagcggta ccaagacggc ctgcagaaaa ccatgcagag gggttgggtg cgggcggttt
     2101 gatgtcgcgc ctgccgagca tgggtgtgct cgttgtgggg cgcgacgcat agcgaggtgg
     2161 tggtgttggg ttgcttgacg gcgccggttg gacgaacgcg atgaggcgtg cggcagcagg
     2221 ctgccgagtc gttgctgcac aacggacacc gtcgatgcgg tatggcggtg cgcttgggct
     2281 acttgtcgag agacggatat ggagctgaag atcatctcgt ggaacatccg ccactttcgt
     2341 gagaagaaaa tcttcgactt cattcacacg ctgtgggaag tcatcgtttg gtgcgacgtt
     2401 tgctttatct atgaggatca catgggcaac ggaacggctc tggccaaggc gttgaacgag
     2461 tacgaatcca agctgccttt ggacaaggtg cgtaaccact ggaagggcga agaaatccat
     2521 gccacggacg aatacgtcgc tattgtttgg aaaagcggcg ttcaggtgac tccacactct
     2581 gctgctacgg cggttttctc ggaactgatg tcgggggagc gctgtcccgc cgcggtgaag
     2641 atcgaaaagt ccgaaaagag tttgatcgtg gcggcatggc acgcctatgg tccggcgaag
     2701 gtttccgcca aagacctgtt ccagaaaatt cttcaggtaa acatttgtga cgtgctcatt
     2761 ggcgatttca atttccaaac cttcgccacc gggcttggtt cgggtggctt gaatgtcacc
     2821 gatgatgatt tttcgctggt ggaatcactg gatttcaagg ttccgcccat gcgcaccagt
     2881 cctgctacca gcttcggctt gagtgcggtg cccagtagtt cgagtgcggt tcctatggcg
     2941 gccagctcca gttcgttctc ttcaccggat ccggtgcctg gcggtaaatc gccgccgatg
     3001 cgtagttcga ggctggaaga taaggttcac atgaaggaaa tcgtgcccgc aaatcatcag
     3061 ggttcgacga cctataccga ggatcgggtg ggcaagcgca ccactgggtt ggatcggtgc
     3121 ctggtccgca agcaattcgt cagttcgtgc aagttgatgg tggccatgcc ggaatggttc
     3181 gacgaattgt tctgtctgac ggatcatatg gttttgcagt tgattatcga tatatgatcg
     3241 aggactaagc gggaagggag cgtatgaacg acaggggggg gcaggctcac ttatgctgac
     3301 ggtgcagtga ataacttgat ctgaccccgt tgctccattt caaatgacga ttgagcgttg
     3361 ggggctgggg tatgccgatt actacgggcg atcttcgga
//
LOCUS       DATMTI010000757         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_749733, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000757 DATMTI010000000
VERSION     DATMTI010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749733"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1261
                     /locus_tag="VN039_12070"
     CDS             110..1261
                     /locus_tag="VN039_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46731.1"
                     /translation="MYIRTNHGLGRFSTTYRYDSSINGIGQISRTPVETGPWDPASEI
                     DQALKAKNWGKALELAIEAGQHDEVELTNLIFFAQNPQLPRAPLDPKAKNFKTLSGQW
                     RTIMTKDVHSAIERMSVDTDLAVDGKFVAERDPQFWGTNGKAFRDIVRSVAAEVDLNP
                     GFLSAVLLAEVGSAAPYLKTGPVFSFFTGTDDFFAQRERLERYVPAFANVHFDKTKTF
                     ESINENGRKVKSIFFDTGKDAARATAVYLAYARLKLQAAAKTNGGDFDALPVQVQFAL
                     MRIAMAAGHGGIELNGNFVWFLKKDGKSLQVKAGTKGAVLVGVARWLYRVLKGEDIFV
                     RDWTGRTFPSTAKATSRNATILVAQALHLSDWHFGIPLVLRQKRPVRPW"
     gene            1378..>1914
                     /locus_tag="VN039_12075"
     CDS             1378..>1914
                     /locus_tag="VN039_12075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWV46732.1"
                     /translation="MSRKTRQARSKVPSRARRAPHFIWPLSKSRKMPDEMNTSYGPRI
                     DADRWDFHDGIDLPASVGTPVHAMAEGIVHRAGPADKTGPGQGFGSTHIILKVVDPID
                     GKDDLFLVYLHLDSIAQGVIPGAEVKPGDIIGAVGQEDATYPHLHFEFRKGGPKEENS
                     VHPMHYLSYPNKGNFTDLD"
BASE COUNT          454 a          492 c          583 g          385 t
ORIGIN      
        1 agatccgaag accttctatg tcaagatggt ttcaaagtcg ctgtcctcac cggactcgag
       61 agggagatga gtcatgctgc gtctccgtcg tctgcgaagg agaactaaca tgtacatacg
      121 gacaaaccat ggcttagggc gattctcaac gacataccgt tatgattctt cgataaatgg
      181 tatcggacag atatcgcgga caccggtcga aactggacca tgggatccgg cgagtgagat
      241 cgatcaagca ctaaaagcca aaaattgggg gaaggcgttg gaactggcga tcgaagcagg
      301 ccagcatgac gaagtcgaac tgacaaatct gattttcttc gcgcagaatc cgcagctgcc
      361 acgcgcgccg ctggatccaa aggcgaagaa cttcaagacc ctgagcggac agtggcgcac
      421 gatcatgaca aaggatgtgc attcggcgat cgagcggatg tcggtagaca ctgacttggc
      481 ggtggacggt aagttcgttg cggagcgcga cccgcagttt tggggaacca atgggaaagc
      541 ctttcgcgac atcgtgcgat ccgtggccgc ggaagtggat ttgaaccctg gcttcttgtc
      601 tgccgtattg ctggcggagg tagggagcgc cgcgccgtac ttgaagacgg gtccggtatt
      661 ctcattcttt acaggaacgg atgacttctt cgcacagcgg gagcgactgg aaaggtatgt
      721 tcctgcgttt gccaacgtgc atttcgacaa gacgaaaacc ttcgagagca tcaacgagaa
      781 tggccgaaag gtaaaaagca tcttttttga taccggcaag gacgcggcgc gggcgacggc
      841 ggtatatcta gcgtatgcgc ggcttaagct acaggctgcg gcgaagacga acggcggaga
      901 cttcgatgct ctacctgtgc aggtgcaatt cgcgctgatg aggatcgcga tggcggccgg
      961 gcacggggga atcgaactaa acggtaactt tgtctggttt ctgaagaagg acggcaaatc
     1021 cttgcaggtc aaggctggga caaaaggtgc tgtgttggtt ggtgtcgcgc gttggctgta
     1081 tcgcgttctg aagggcgagg atatcttcgt tcgggactgg acaggccgca cctttccgag
     1141 cacggcaaaa gcaacgtcgc gaaacgcaac gattctggtc gcacaggcgc ttcatttgtc
     1201 ggactggcac tttgggattc cgttggtgct acggcaaaaa cggccagtgc gtccctggta
     1261 gcacaaggta ccgacgcggg agtggattag gtcagagagg gtccagcgtt gcactcaggt
     1321 atctggaagc tcagatcctc cacgagtgcg catacatcga gcgtaaggag atggaccatg
     1381 agccgcaaaa caagacaagc tcgctcaaaa gtcccgtctc gcgctcgacg cgcccctcat
     1441 ttcatctggc ccctgagcaa aagtcggaaa atgccggatg agatgaacac gtcctatggc
     1501 ccgcgtatcg atgcggatcg ctgggacttc cacgacggca tcgacctgcc cgcttccgtc
     1561 ggcaccccgg tccatgccat ggcggagggc atcgtgcatc gcgccggacc ggcggataaa
     1621 accgggccgg ggcagggctt cggctcaacc catatcattc tcaaagtcgt tgatccgatc
     1681 gacggcaagg atgacttatt tcttgtctat ctccatctcg acagcatcgc ccaaggtgtg
     1741 atcccgggtg cagaggtgaa gccgggtgac atcatcggcg cggtgggtca ggaagatgcg
     1801 acctaccccc atctccactt cgagttcagg aaaggagggc ccaaagaaga gaacagtgtg
     1861 catccgatgc actatctgag ttaccccaac aaggggaact ttacggatct cgac
//
LOCUS       DATMTI010000758         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_538264, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000758 DATMTI010000000
VERSION     DATMTI010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538264"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..646
                     /gene="tmk"
                     /locus_tag="VN039_12080"
     CDS             92..646
                     /gene="tmk"
                     /locus_tag="VN039_12080"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWV46733.1"
                     /translation="MLHTREPGGTLLAERLRNLLLDHSVETIAPETEAYLIFAARRQH
                     VDHVIKPALTRGMTVVCDRFSDSTMAYQGYGRGLDLRVLRTMNDWATGKLAPQLTLLF
                     DVPVSVGLSRRRSRAATQNRLDRETERFHRKVRAGFHALVRREPRRIVVINSARPLES
                     VAQAVEGLVMNWLKTYRVPASRHR"
     gene            650..1678
                     /gene="holB"
                     /locus_tag="VN039_12085"
     CDS             650..1678
                     /gene="holB"
                     /locus_tag="VN039_12085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HWV46734.1"
                     /translation="MPFADITGHEQPISLLQASLCNGRLAHAYLFYGETRVGKLMTAI
                     RLAQALNCEQPAQTETYDSCGRCRSCLQIAARTHPDYFVIEPDPKAAMPQIKIEQVRE
                     IEQQFVYRPLVGERKICLIDDADRLTIGAANALLKTLEEPPGQSLFILITSRLYALPV
                     TIRSRCQALRFTTPARTQVEAALILKRELPPVDARFLAVFSDGRIGEALTVNVAEVRA
                     RQQECLTLVKPESLTSSTTILSASESLAKTDRGEETLAWLTRWIRDLVFVIVDGDQDQ
                     ILHLDQLADLRRYAQRADIDTLLTLLNDIERTEQQATRHLNVQMALETTFLRLREALG
                     LAPAGASA"
     gene            1715..>2227
                     /locus_tag="VN039_12090"
     CDS             1715..>2227
                     /locus_tag="VN039_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWV46735.1"
                     /translation="MSRDDIFYVTTPIYYVNDVPHIGHAYTTVAADVLTRYWRLRGHE
                     VFFLTGLDEHGQKVQQAAAKAGIAPQAHCDKLAPKFETLWKKLNVSNNAFIRTTDPEH
                     KSIVQRYLQQLYDKKLIYKDAYTGWYCTFDERFWTEKDVESGLCPDCKRPVEKLSEHN
                     YFFKMGQYQDR"
BASE COUNT          516 a          661 c          609 g          441 t
ORIGIN      
        1 gcatcttcat cacgctggaa ggcggtgaag gaagcggaaa acgacacaag ctcgaagact
       61 gtgtgagcgg ctgaccgcgc aaggcctgga cgtgttgcat accagagaac cgggagggac
      121 gcttctcgct gaacgtctgc ggaatctcct gcttgaccac tcggtggaaa caatcgcccc
      181 ggaaacggaa gcctacctca tttttgcggc aaggcgtcaa catgtggatc acgtcatcaa
      241 acccgcactc acacgcggga tgacggtcgt gtgtgatcga ttttcagact cgacgatggc
      301 ctaccagggg tatggacggg gattggatct acgagtgttg cgcacgatga acgattgggc
      361 gaccggaaag cttgctcccc agcttacctt gctcttcgac gtccctgtaa gtgtcgggct
      421 cagtcgacgc cgtagtcgcg ctgcgaccca aaatcgactc gatcgagaaa ccgaacggtt
      481 ccaccggaaa gtccgtgcgg gatttcacgc gttggtacga cgtgaacctc ggcgcatcgt
      541 ggtgatcaat tccgcacgac ctctggaatc agtggcacaa gccgtagaag gactggtcat
      601 gaactggctc aagacctatc gcgtaccggc atcgagacac cgatagccca tgcctttcgc
      661 cgatattacc ggtcacgagc aacccatctc cctacttcag gcgtccttgt gcaatggtcg
      721 cttggcccat gcgtatctat tctacggtga aaccagagtc ggaaagttga tgaccgccat
      781 acggctggct caggccctca actgcgagca gcccgctcag acggagacct acgacagctg
      841 tgggcgttgc cgatcctgcc tgcagatagc cgcacggacg cacccggatt acttcgtcat
      901 cgagcccgac ccgaaagcag caatgccgca aatcaaaatc gagcaagtgc gggagatcga
      961 acaacagttc gtttatcgac cactcgtcgg ggaacggaag atttgcctga ttgacgacgc
     1021 ggaccgactg acgatcgggg cagccaacgc gctcctcaaa actttggaag agcctccggg
     1081 tcagagtctc ttcatcctca tcaccagcag gctctatgcg cttccggtca ccattcgatc
     1141 acgctgccaa gcgctccgtt tcacgacgcc cgcccgcacg caagttgagg cggcgctgat
     1201 cctcaagcga gagcttcctc ctgtcgatgc ccgcttcctg gcggtctttt ccgatggccg
     1261 tatcggagaa gcgctcacgg tgaacgttgc agaagttcga gcgcgacagc aagaatgcct
     1321 gactttggtg aaaccggaaa gcttgacgtc gagcacgacc atcctctctg catcggaaag
     1381 cttggccaag acagaccgtg gagaagaaac gctcgcgtgg cttacgcgat ggatccgtga
     1441 tcttgtcttt gtcatcgtgg atggagatca ggatcagatc cttcacctcg accaactcgc
     1501 cgacctacga cgttatgccc agcgagcgga tatcgacacg ctcctgaccc tcttgaatga
     1561 catcgagcgg accgaacaac aggccacgcg gcacttgaac gtgcagatgg cactggaaac
     1621 gacgttcctt cgcctgcgcg aggcactcgg gctggccccc gccggagctt ccgcctagta
     1681 ataaacgatg ccaacctgat atcttgcctt cgttatgtct agagatgaca tcttttacgt
     1741 caccacgccg atttattatg tcaacgatgt cccgcatatc ggccatgcct acaccacggt
     1801 ggcggcggac gtgctgacgc gctactggcg gctgcgtggg cacgaggtgt tttttctcac
     1861 cggcctcgat gaacatggcc agaaagtgca acaggcagcc gccaaggccg gtatcgcccc
     1921 acaagcccac tgtgacaagc tcgcgccgaa atttgagact ctctggaaga agctgaacgt
     1981 ctcgaataac gccttcatca ggacgacgga tcccgagcac aaatccatcg tccaacgata
     2041 cctgcagcag ctctacgaca aaaagctgat ttacaaggat gcatacaccg gatggtactg
     2101 caccttcgat gaacggttct ggaccgaaaa ggacgtggag tctggactct gtcctgactg
     2161 taagcgcccc gtcgagaaac tcagcgagca caactacttc ttcaagatgg ggcagtatca
     2221 ggaccgt
//
LOCUS       DATMTI010000759         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_199474, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000759 DATMTI010000000
VERSION     DATMTI010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199474"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VN039_12095"
     CDS             <1..306
                     /locus_tag="VN039_12095"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HWV46736.1"
                     /translation="VRNHTHDICPFFDTTWDFLDFSDEIGAVRPLVPKPANLAEMLTL
                     AAKLSVGFGYVRLDFYNVKGRVYFGEFTFTPAAGIITYDPECWDLKLGEKWDLSLDC"
     gene            579..>1536
                     /locus_tag="VN039_12100"
     CDS             579..>1536
                     /locus_tag="VN039_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46737.1"
                     /translation="MSRWMALGLWALFLMRVVVVCVTADGYVAHASHDGQSAAEIHNP
                     SGSGYQVPGLSFRVFVDRGVEEAVGGLLGQAQADAALRTVIETFSYLNQHRHTYPRFD
                     EAVSKGRLERVIIEPTIHNHEGKAFPFLVVRTMNPGRVKLLISASSMREGQYLGEPEQ
                     FAPVLAREFQWVVSKAETGQKPKTGFHERDLRHAPIRTDKEIRAFSGEERVRLLQQLF
                     KTYLYTVDDQQSLDGQSYYEIGSTILVPPSQPDSTTKFYDIRVREALQQIIREPSFLE
                     RTPLAVTNLLNGNIWNVAFVKVDQRDWATRTRVQPADKAVRLG"
BASE COUNT          344 a          385 c          429 g          378 t
ORIGIN      
        1 gttcgtaatc atacacacga tatctgccca ttttttgata ccacatggga tttcctcgac
       61 ttttccgatg agataggtgc ggtacgtccg ttggtaccaa agcctgcgaa tcttgccgag
      121 atgttgacgc ttgccgcgaa gctgtcggtc ggcttcggct acgtccgctt agatttctac
      181 aatgtcaaag ggcgagtcta ttttggcgaa tttaccttta cacccgcggc agggatcatc
      241 acatatgatc ccgagtgttg ggatctgaag ctcggggaga aatgggatct gtcattggac
      301 tgctgagagc cacgtcgatt tcatgggtgg ctcacccgtt gatcgatgga tgtaattgct
      361 atccccggcg catgtagcta gtgttggcat ctcccgatct gaccggatat agccgtcttt
      421 ccttccatag cgacttgtaa aagcggttgt acgttgcact gaagaccttc ctatttgaac
      481 cgatgggctc catctctttc tgcctcgcac tccagaagtc accatgacat aatcggcttg
      541 agcgcttcgc ccaatctctc gactcaaaag gatcgagcat gtctcgttgg atggcactgg
      601 gtctgtgggc gttgttcttg atgcgcgtcg tggtggtctg cgttaccgct gatggctatg
      661 tagctcatgc aagccatgat gggcagtctg cggcagagat ccataatccc tcaggatcag
      721 gctaccaggt tccaggtctt tcgttccgag tatttgttga tcgcggagtg gaggaggcgg
      781 tgggtgggct tctcggccaa gcccaagccg acgcagctct ccggacggtg atcgagacat
      841 tctcctattt gaatcaacat cggcatacct atccacggtt cgatgaagcc gtgagcaagg
      901 gccggctcga gcgcgtcatt atcgaaccta caatacacaa tcatgaagga aaggcgtttc
      961 cgtttctggt tgtgcggacg atgaatcccg gtcgggttaa gttattaatc agtgcgtcgt
     1021 cgatgaggga gggacaatat cttggtgagc cggaacagtt tgctccggtg ctggcgcgag
     1081 agttccaatg ggtggtcagc aaagcggaga ccggtcagaa gccgaaaacc ggcttccacg
     1141 aacgtgacct tcggcatgca ccgattcgaa ccgacaagga gattcgggcc ttctcgggtg
     1201 aagagcgggt acggctgctc caacagctat tcaagacgta cctatacacg gttgacgacc
     1261 aacaaagttt ggacgggcag tcctactatg agatcggcag caccattttg gttccaccaa
     1321 gtcaacctga ctctacgacg aagttttatg atattcgggt ccgagaagcc ttacagcaaa
     1381 tcattcgcga gccgtcattt cttgaacgca cgccactggc cgtaacaaac ctcttgaacg
     1441 gaaatatctg gaatgtggcg ttcgtcaaag ttgaccagcg ggattgggcg acaagaacga
     1501 gagtgcagcc ggcagataaa gccgtgaggc tcggtg
//
LOCUS       DATMTI010000760         1835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_326166, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000760 DATMTI010000000
VERSION     DATMTI010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326166"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VN039_12105"
     CDS             <1..1165
                     /locus_tag="VN039_12105"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46738.1"
                     /translation="IDDYVPTMLVASSNTKVSQYTMDDLEALGYLKDDVLGQRTLWVM
                     ARCQELMGMQDKTDFSWIPLNDAATCSMLSEGRTNNAVFQFEGYAMATGARSMGIRNT
                     SDCVLAGALFRPACIDSGVTELYIRRRNSTVLRSNLRYPHPAFEKALKSTHGCVLFQE
                     QDLQIMRDLGVDYAGINKFFEIVKDSGKGATERNIERAMEVRKQWDDICKRNNITDRE
                     AAWHYIEGYVKYGFNAAHATGYGVRSYRAGYLKTHYPLEYMTAVLESVAGKSTGKYDK
                     EALYIKEARRIGIRILPPDVMLSGALWTMDSRRKAITKGLASIKSVKAAAYALVEARD
                     QLPFKSVQDMAERLPARVLTGRDAFLRKGEWTGVMKALKLAGALESLGVNRED"
     gene            1165..1404
                     /locus_tag="VN039_12110"
     CDS             1165..1404
                     /locus_tag="VN039_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46739.1"
                     /translation="MPEIVKLVFTLKDGKEMEPGVSPASGHIDIDLGATTACAKVLSQ
                     SVAEDRRTVTLELEVPDGILPATSNMGSFYSVGEN"
     gene            1404..>1835
                     /locus_tag="VN039_12115"
     CDS             1404..>1835
                     /locus_tag="VN039_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46740.1"
                     /translation="MPAKSKPEEAPPSNARELMDFVNQQFGAGTLTMASDPRWQIQML
                     PTGLSPIDHLLKGGVPYGRFVVFHGDYSSLKSYIGLCAIASAQKAGKLAAIIDTEHAF
                     EPKWAKSLGVDLENLIIRQPETGEQAIDTAEALIRGGVDVIV"
BASE COUNT          424 a          477 c          559 g          375 t
ORIGIN      
        1 gatcgacgat tatgttccga ccatgctggt agccagtagc aacaccaaag tcagccagta
       61 cacgatggat gacctggaag cgcttgggta cctgaaagac gatgtactcg gacagcgtac
      121 tttgtgggtc atggcacgct gccaggaact catgggtatg caggacaaga ccgacttctc
      181 ctggattccg ctgaacgacg cggcaacctg ctccatgctc tcagaaggta ggaccaataa
      241 tgccgtcttc cagtttgagg gatacgccat ggcgacgggt gcacggtcta tgggtatccg
      301 aaacacctcc gactgtgtac tggccggagc tttattccgc ccagcctgta ttgattccgg
      361 agtcacggaa ttgtacattc gccgcaggaa ctcgacggtg ttacggagta atctgcgtta
      421 cccgcatccc gctttcgaaa aggcgctgaa gtccactcac ggctgcgtgc tattccaaga
      481 gcaggatctt cagatcatgc gggacttggg tgtggactac gcgggaatca acaagttctt
      541 cgaaatcgtg aaggattccg gcaagggagc aaccgagcgg aacatcgaac gggcgatgga
      601 agttcgcaag cagtgggatg atatctgcaa gcgaaacaac atcactgacc gggaagctgc
      661 gtggcactac atcgaggggt atgtgaagta cggcttcaac gccgcgcacg ccaccggcta
      721 tggtgtccgg tcctaccggg ctggatactt gaaaacgcac tatccgctgg agtacatgac
      781 ggcggtgctg gagagcgtgg ccggaaaatc caccggaaaa tacgacaagg aagcgctgta
      841 catcaaggaa gcgcggcgga tcggaatccg catccttccc ccggatgtca tgctgtccgg
      901 cgcgctctgg acgatggact cccggcgtaa ggcgatcacc aaggggctag cgagcatcaa
      961 gagtgtcaag gcagccgcgt acgccctcgt ggaggcgcgg gatcagcttc ccttcaagtc
     1021 ggtacaggac atggctgaga ggctgccagc gcgcgtcctg acgggtcggg atgccttcct
     1081 gcgcaagggt gagtggaccg gggtgatgaa ggcgctcaag ctggctggag cgctagaatc
     1141 actcggagtg aaccgggagg attgatgcct gaaattgtga agttagtttt cacgctcaaa
     1201 gatggtaaag agatggagcc gggtgtttca ccggcaagtg gacacatcga tatcgatctt
     1261 ggagcaacga cggcttgcgc taaagttctc agccagtctg ttgcggagga ccgtcgtact
     1321 gtcactttgg agttggaagt tccggatgga atccttccag ctactagcaa catgggctct
     1381 ttctattcag ttggagagaa ctgatgcccg ccaaatcgaa gccagaagaa gctcccccga
     1441 gcaacgcccg cgaactgatg gacttcgtca atcagcagtt cggtgcaggc acactgacga
     1501 tggctagtga cccgcgctgg cagattcaga tgttgccgac cgggctttct ccgattgacc
     1561 acttgctcaa gggcggtgtt ccctacggca ggtttgtagt tttccacgga gactacagct
     1621 cgttgaaaag ctacatcggt ttgtgcgcta ttgcttcggc gcagaaggct ggaaaactgg
     1681 ccgccatcat cgacactgag cacgccttcg aacctaagtg ggccaagagt ctcggcgttg
     1741 atctggaaaa cctcatcatc cggcagccgg aaaccgggga gcaggcgatc gacactgcgg
     1801 aagctctcat tcgtggcggg gtagatgtga ttgtg
//
LOCUS       DATMTI010000761         1632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_326182, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000761 DATMTI010000000
VERSION     DATMTI010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326182"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VN039_12120"
     CDS             complement(<1..516)
                     /locus_tag="VN039_12120"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWV46741.1"
                     /translation="MPLPNETVASLAMALVQSSKAPLLLLDDDAVVIGASDSFCNTFN
                     LDPTTIVNRKLVDLGTGEWDVPQLNSLLRATITGAADIDAYEMDLVRKGKETCRLILS
                     AHKLDYFDIGKARVVLAATDITAARLAEKQKDDLIREKQVLLQELQHRVANSLQIIAS
                     VLMQSARRVSSE"
     gene            complement(521..874)
                     /locus_tag="VN039_12125"
     CDS             complement(521..874)
                     /locus_tag="VN039_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46742.1"
                     /translation="MHALIIEDESMIALAIEDALRGCGFASFDIAVSAEEAVAAAERR
                     CPDLITADVELRPGCGITAVQSICSERPIPVIFITGSPGEVRIRMPGHALVEKPFSAD
                     HIRDATKLTLDSRDG"
     gene            complement(1162..1377)
                     /locus_tag="VN039_12130"
     CDS             complement(1162..1377)
                     /locus_tag="VN039_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46743.1"
                     /translation="MRAPAAAGDLDMSPNEREYYRERALIERDRASTATSVAAPIHLE
                     LACLYEKLVELEDGHEVPILRVVGSKR"
BASE COUNT          329 a          478 c          447 g          378 t
ORIGIN      
        1 ctcggacgaa acgcgccgag cactttgcat caacacgctt gcgatgatct gcaggctgtt
       61 ggcgacacgg tgctgaagct cctgaaggag aacctgcttc tcacggatga ggtcgtcctt
      121 ctgcttttcg gccaggcgag ccgccgtgat atcggtcgcc gcgagcacga cacgcgcttt
      181 tccaatatcg aaatagtcga gcttgtgagc acttaatatg agacgacacg tctctttgcc
      241 cttgcggaca agatccatct cataggcgtc aatatcggct gctccagtga tggtagcacg
      301 aagcagggag ttgagttgtg gaacgtccca ttcaccggtc cctaaatcga ccagcttgcg
      361 attgacgatc gtcgtcgggt caagattgaa cgtgttacag aatgagtcgc ttgcaccaat
      421 gacgaccgca tcgtcatcga gcaggagcag cggagccttc gatgactgaa ctagggccat
      481 ggccagactg gccactgtct cgttggggag aggcatcgtt tcaaccatcc ctgctgtcca
      541 aggtcagctt ggtcgcatct cttatatgat ccgcgctgaa tggcttctca accaatgcat
      601 gccccggcat ccttatcctg acctctccgg gacttccggt aatgaaaatg accggtattg
      661 gccgctcaga acaaattgac tgaacggccg tgatgccaca gcccggcctt agctcgacgt
      721 cggcggtgat caggtccgga caccttcgct ctgccgccgc caccgcttcc tccgccgaca
      781 cggcgatgtc gaaggatgcg aagccgcagc cacgcaacgc gtcctctatc gcaagagcga
      841 tcatggattc gtcttcaatg atgagtgcat gcatcacatc gctccgtgag cggggagcgt
      901 gccacgtctc tccagcacca aagcccaact taactcgttg gtgataccgt attaacgggg
      961 agaagtatca ttcgttgcag gacgccttcc gattgactgt ggcgaccggt tggaatctac
     1021 gttccacccg ctcctgccat agctcggcat tggcagcttt ggacccatct acgacattcg
     1081 acccttaggt ctgctttcga tgagatgcac cggctaccga cgccttcccg aatctgggtc
     1141 tgcagcggaa gctggactaa cttatcgctt cgacccgacg acgcgcagga tcggcacctc
     1201 gtgcccgtcc tcaagctcaa ctagcttttc ataaaggcaa gccagttcga gatggattgg
     1261 ggcggctact gaagtcgcgg tggaggcgcg gtctcgttcg atcagcgcac gctctcggta
     1321 atattcgcgt tcattgggcg acatatcgag gtctcctgcc gcagcgggag ctctcaattg
     1381 tctctcgttc gccgactcgc tgaattttga ggtcggcgat agcgcttgtt acgcctcttt
     1441 cgtggattcg cagaatttgg cggaaacgtg cgcttcacat tgtagaggtt gcacgtcgct
     1501 taagcccggg acttcaacag ccttttcaga tcccggcgaa gcgagtcctt cagatgggcg
     1561 tcaatattcg tgcagccctc ctggtgcagt ttggcgcgga gctcttccat cgtttcgcat
     1621 tgcggagcca cc
//
LOCUS       DATMTI010000762         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_199516, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000762 DATMTI010000000
VERSION     DATMTI010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199516"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VN039_12135"
     CDS             complement(<1..119)
                     /locus_tag="VN039_12135"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWV46744.1"
                     /translation="MSECLFCQIVEKKISAKLVQEDEHTLAFDDINPQAPVHT"
     gene            complement(182..850)
                     /gene="hisIE"
                     /locus_tag="VN039_12140"
     CDS             complement(182..850)
                     /gene="hisIE"
                     /locus_tag="VN039_12140"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HWV46745.1"
                     /translation="MSQETTDQFKFDGEGLLPVVIQDWLDGTVLMLGYMNQEAFAKTV
                     STKRVHFWSRSRNKLWEKGETSGHTLHVKELFLDCDCDTILVKAQPAGPTCHTGERAC
                     FFSKMDEQGHVVHRSAYHAQGGILESVLRIIRERRSSPQPGSYTAKLFEEGHDKILKK
                     VAEEAGEVLLASKGGKKEEIVHEVADLFFHTLMVLGYHDLPLQDIYEELGGRFGKPSL
                     RSGQ"
     gene            complement(855..1685)
                     /gene="hisF"
                     /locus_tag="VN039_12145"
     CDS             complement(855..1685)
                     /gene="hisF"
                     /locus_tag="VN039_12145"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWV46746.1"
                     /translation="MTANWIIKPLWRRFPCDMLTKRIIPCLDVKEGRVVKGVSFVNLR
                     DAGDPVEAAVGYDHEGADELCFLDITASHENRKTIIDVVERTAARVFMPVTVGGGVGT
                     LDDIRALLNAGADKVSINTAAVRRSEFVKEAARRFGTQCIVVAIDAKRVTGDRWEVFT
                     HGGRHATGLDAVEWAKRMEEYGAGEILLTSMDQDGRQTGYDLNLTAAVSGSVSIPVIA
                     SGGVGALDHLYDGFVKGKADAVLAASIFHFQTYTISQAKAYLRERGVPVRSDTLPRVA
                     "
     gene            complement(1636..2358)
                     /gene="hisA"
                     /locus_tag="VN039_12150"
     CDS             complement(1636..2358)
                     /gene="hisA"
                     /locus_tag="VN039_12150"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWV46747.1"
                     /translation="MLIIPAIDLKDGRCVRLRQGDMAAETIYSDDVTEVAERWQQRGA
                     GLIHVVDLNGAVDGEPKNLPQIESIIKAVHVKVQVGGGIRTMDTVRRYLNAGVSRVVL
                     GTAALTNRTLLNLACREFPQRIVLGLDARDGRIAVKGWTAVSDMKAIDLLKELSGCAI
                     AAVVYTDIARDGMLNGPNLPALKEITEFSSFPVIASGGITSLEDLRAVQSISPKIEGA
                     IIGKALYDGELDYQAAVAALPL"
     gene            complement(2360..>2875)
                     /locus_tag="VN039_12155"
     CDS             complement(2360..>2875)
                     /locus_tag="VN039_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46748.1"
                     /translation="SDISLSVPATVEPPPKQTMIVPEYAAQKVTELFWGRLQDRNGIH
                     VLSPSDSARASLADGAPIGAKPEQVAAAVAQRLKADATLIGRVLVYQERVGSRLGADP
                     TATVGFEAKVVASDGQVLWVGNYYERQRPMGEDIMGFVHRWAFVTAAELAEYGVDEVL
                     KEFPFGRGEER"
BASE COUNT          628 a          914 c          682 g          651 t
ORIGIN      
        1 aacgtatgaa cgggggcttg aggattgata tcgtcaaaag ccaatgtgtg ctcatcctct
       61 tgcaccagct tcgccgaaat cttcttctcc acgatttgac aaaaaaggca ctcgctcatc
      121 tcagcctcta caacaattga acggcatcga tccgcctcag aatctctatg accctattgg
      181 gttactgccc tgaccttaga ctcggttttc caaatctgcc tcctaattct tcataaatat
      241 cctgcaaagg caggtcatga taccccagca ccatgagggt gtggaaaaac aaatcggcta
      301 cttcatgcac gatttcttct ttcttgccac ctttggacgc caacaggact tctccggcct
      361 cctccgcgac ctttttcaga atcttgtcat gcccctcctc gaacaacttt gcggtatagg
      421 aaccgggctg tgggctggag cggcgctccc gaattatccg caggacactt tcaaggatcc
      481 ccccctgcgc gtgatacgca ctccgatgaa cgacatggcc ttgttcatcc attttagaga
      541 agaagcaagc ccgttcgcct gtatgacagg tcggcccggc tggttgcgcc ttcaccagaa
      601 ttgtgtcgca gtcgcagtca agaaagagtt ccttcacatg tagcgtatga ccggacgttt
      661 cgcctttttc ccagagcttg ttccgagatc gactccaaaa gtgcactctc ttggtcgaga
      721 ccgtcttcgc gaaagcctct tggttcatat accccaacat cagcaccgtc ccatcaagcc
      781 aatcctgaat gacgaccggg agaagcccct cgccgtcgaa cttgaactga tctgtcgttt
      841 cctgactcat cgtgtcatgc cactcgagga agagtatcgg atcggacagg gacaccacgt
      901 tcgcgcaaat acgctttcgc ttgggagatc gtataggtct gaaaatgaaa aatcgaagcg
      961 gccaagacgg cgtctgcctt ccctttcacg aaaccatcgt ataggtggtc gagcgccccg
     1021 actccacccg atgcaatcac gggaatcgac acggagcctg acacggcagc tgtcagattc
     1081 aagtcatacc cggtctgccg gccatcctga tccatacttg tgagtaaaat ctctccggcg
     1141 ccgtactctt ccatccgttt cgcccactca accgcatcaa gtcccgtcgc atggcgaccg
     1201 ccgtgcgtga agacctccca gcgatcaccc gttacgcgct tggcatcgat ggcaacgacg
     1261 atgcattgcg tcccgaaacg ccgagcggcc tctttcacga actcggaccg tcgtactgcc
     1321 gctgtattga tgctgacctt atctgctccc gcattcaaca aggcccgaat gtcgtcgagc
     1381 gtccccacgc cgccaccgac tgtcaccggc atgaataccc tggcggccgt ccgctcaacg
     1441 acgtcgatga tagtcttccg gttttcatgg gaggccgtga tatcgagaaa acagagttcg
     1501 tctgcgcctt cgtgatcata ccccactgcg gcttcaaccg gatccccggc atctcggaga
     1561 ttcacaaagc tgacgccttt gaccacgcgg ccctctttga cgtccagaca ggggatgatg
     1621 cgcttggtca acatgtcaca ggggaagcgc cgccacagcg gcttgataat ccagttcgcc
     1681 gtcatagagt gctttgccga taatcgctcc ctcgatctta gggctgatcg attgcactgc
     1741 tcgcagatcc tccagagacg tgatgcctcc cgacgctatt accgggaacg aagagaattc
     1801 ggtgatttct ttcaacgcgg ggagattcgg tccattcagc atgccatcgc gtgcgatgtc
     1861 tgtatacact acggcggcaa tcgcgcagcc tgaaagttcc ttcagcaaat cgatcgcttt
     1921 catatcggat accgctgtcc agccttttac cgctattcgg ccatcacgtg cgtcgagacc
     1981 aagcacgatc cgctgcggaa actcccggca agcaagattg agaagcgtcc ggttcgtgag
     2041 tgccgccgtg ccaagcacga cacgtgacac cccggcattg agataccggc gaaccgtatc
     2101 catggtcctg ataccgccac cgacctgaac ttttacgtga accgctttta taatggactc
     2161 gatttggggc aggttcttgg gctcgccgtc aaccgcgccg ttcaaatcca cgacgtgaat
     2221 cagaccggct cccctttgtt gccacctttc ggcaacttcc gtgacatcgt cggaatagat
     2281 tgtttcagcc gccatatcgc cttgacgcag ccgtacacag cgtccatcct tcaagtcgat
     2341 cgcgggaata atcaacatgc tatcgttcct ctcccctgcc gaacggaaat tccttcaaca
     2401 cctcgtccac cccatactct gccaattccg ccgccgtcac gaaagcccat cggtgtacaa
     2461 atcccatgat atcttctccc atgggcctct gccgctcata gtagttaccg acccagagca
     2521 cttgcccatc cgatgccacc accttggctt caaagccgac ggttgccgtt gggtctgcgc
     2581 ccagtcgact gcccacccgt tcctggtaca ccaggacgcg accgattaac gttgcatccg
     2641 ccttcagtcg ctgagccaca gcggcagcaa cctgttcggg cttcgctcca atcggtgccc
     2701 catcggccag cgaagccctg gcagaatcac tgggagacaa cacgtgaatc ccatttcgat
     2761 cctggagacg cccccagaac aattccgtca ccttctgcgc cgcatattcc ggtacaatca
     2821 tggtttgctt gggcggcggt tccacagtcg caggaacaga cagagagatg tccga
//
LOCUS       DATMTI010000763         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_538404, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000763 DATMTI010000000
VERSION     DATMTI010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538404"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VN039_12160"
     CDS             <1..180
                     /locus_tag="VN039_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46749.1"
                     /translation="MPVVGSPSDHPHGHAVVVFDAETGQRIGHNPNYVVEQSRLKKVD
                     GGFSMTLKLRHIKDS"
     gene            180..1463
                     /locus_tag="VN039_12165"
     CDS             180..1463
                     /locus_tag="VN039_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46750.1"
                     /translation="MFLKAPADVLDFTWDWSAWMQAGDSISTVNWEIPETFLPPPSGL
                     IANTSTNGGTFAANAYYWVVTATNAAGETTPSNEISATLTGTTSSVGLGWSNVVGATG
                     YNIYRGSNTSGTPVTVDSGASGTKIGNASGSTSSLTATCTYTKGTANYVVASVGFLAG
                     LAPNIMTCSYGSTPMTYLGSSISGFGGTAFFGLASPPSGSQTVTANVQSFGNVIGLVV
                     AVASFAGVGSVVYGAGQGGQIGSPSQNVSAVYGDLVVWAATNVTGSSINAAMSSFNQT
                     SLENSGNVSLPIADQIGHLLGYASGPTSGSSSLAFTASVAQANIDQWWSSGALTLLPA
                     APPINTLVANVPSSTTQYTDTGTAGSSASPPAANTAVTSISPASSPAASHTTDTATAW
                     IANGTAGNSYPVTCQIITSEGRVAQNTQTITVENL"
     gene            1558..1824
                     /locus_tag="VN039_12170"
     CDS             1558..1824
                     /locus_tag="VN039_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46751.1"
                     /translation="MPKSAQPSKKLPQKPWAKLEDQDKAALEMLIAEIGCLEYPIKGG
                     VQYLKHPYFQPRFSSDPTATLETTRRFCEALILWFRKNGLLKDG"
     gene            1835..2017
                     /locus_tag="VN039_12175"
     CDS             1835..2017
                     /locus_tag="VN039_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46752.1"
                     /translation="MDGDKLRTLLADANAAHKYATFLDDFIIPLIKKIGLKVSVKKNL
                     KSYVITVRLPNAVQNG"
BASE COUNT          506 a          716 c          661 g          495 t
ORIGIN      
        1 atgcctgtcg tggggtcccc gtctgatcat ccccatgggc acgcggtggt ggttttcgac
       61 gctgaaaccg ggcagaggat tgggcataac ccgaactatg tggtcgaaca gtcgcgcctg
      121 aaaaaggtgg acggtggttt ctccatgaca ctcaagctcc ggcacataaa ggattcttga
      181 tgttcctcaa agcacccgct gatgtcctgg atttcacgtg ggactggtcg gcgtggatgc
      241 aggctgggga ttcgatttcg acggtgaatt gggagatccc cgaaaccttc ctcccacccc
      301 cctcggggtt gattgccaac acttcaacta atggtgggac gttcgcggct aacgcgtatt
      361 actgggttgt caccgcaacc aacgcggccg gcgaaacaac cccgtcgaat gaaatctccg
      421 ccactctgac cggcaccacc tcaagtgtgg ggttgggttg gtcgaacgtt gtcggtgcta
      481 cgggctacaa catttaccgc ggctcgaata cgtcgggcac gccggtcacg gtggattcgg
      541 gtgcttcggg cacgaagatc ggcaacgcct ccggctcaac ctcatctttg accgctacct
      601 gcacctacac caaaggcaca gcgaactatg ttgtggcctc agtagggttt ttggcggggt
      661 tggcaccgaa catcatgacc tgttcgtatg gctcaacccc catgacttac cttggctcaa
      721 gcatttcggg gttcggtggg acagcgttct tcggcctcgc atcgccgcct tctggtagtc
      781 agacggtgac ggcgaatgtt caatcgttcg gcaacgtcat cggcttggtt gtggctgtcg
      841 catcattcgc tggtgtcggg tctgtggtgt atggggctgg tcagggcggc cagatcggct
      901 caccctcaca gaatgtttcg gcggtttatg gtgatcttgt tgtgtgggct gccacgaacg
      961 tcaccggatc ttcgattaac gcagccatgt ccagcttcaa ccaaacatcc ctggaaaaca
     1021 gcgggaatgt cagcctgccc atcgcagacc agatcggcca cctactcggg tatgcgtccg
     1081 gacccacatc cggatcctca agtctcgcct tcaccgcttc tgtggctcaa gccaacatcg
     1141 accaatggtg gagctcaggc gcattaactc tgctgccggc agccccaccc attaacacgc
     1201 ttgtagcgaa cgtcccctcg tccaccaccc aatacaccga cactggtact gcgggtagct
     1261 cagcgtctcc accggccgcg aacaccgcgg tcacgagtat cagcccggct tcttctcccg
     1321 cagcttcaca cacaacggac acggcgacgg cgtggatagc taacggcacc gcaggaaata
     1381 gctacccggt gacttgtcag atcattacgt ctgagggcag ggtcgcgcag aacactcaaa
     1441 cgatcacggt ggagaatctg tgaacccgga aagaaccggc cacgtcctgg ccaatgaccg
     1501 cacccatcag gtgtatgtcc tggatactcg tgtgttcgtg gttcccgaag gcgaataatg
     1561 cccaaatcag cgcagccatc taaaaaactt ccccagaagc cttgggccaa gttagaggac
     1621 caagacaaag cagcgttgga gatgctgatc gctgagattg ggtgtttgga gtacccgatc
     1681 aagggtggtg tgcagtattt gaaacacccc tacttccaac cccgattctc ttcggacccc
     1741 acggcgacgt tggaaaccac gagacggttt tgcgaagcgt tgatcctgtg gttccggaag
     1801 aacgggctgt tgaaggatgg ttagccaaca cggagtggac ggagacaaac tccgcaccct
     1861 actcgcggat gcgaacgcag cccacaaata cgccaccttc ctcgacgact tcatcatccc
     1921 cctgatcaaa aagatcggct tgaaagtcag cgtgaagaag aacctcaagt cgtatgtgat
     1981 tacggtgcgg ctgccgaatg ccgtacagaa cggctagtcc ctgcactttc cccggctgcg
     2041 gtggtcgtgc ggtggttcgt ggccgctgca acaggcataa gcgtggcacc acagtccagc
     2101 gcggctatga cgccacacac aaacgaacac gcgcctccta cgagatagct gttcttcaag
     2161 ggcgtgtgtc gtgttggcgc tgtggtgacc ccatccttcc caccgatgag tgggaccttg
     2221 ggcacgacga ccacgaccgc tccatcaccc gcggccctga acacgcgggc tgcaaccgtt
     2281 ccgcagcctc ccgcctccgt aacgcatagg agcattgaac gtgccgttct acaacatccc
     2341 tagcgacaac cctgtggtcc cattcaagtc gggtcagg
//
LOCUS       DATMTI010000764         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_538449, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000764 DATMTI010000000
VERSION     DATMTI010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538449"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1700
                     /gene="pgm"
                     /locus_tag="VN039_12180"
     CDS             54..1700
                     /gene="pgm"
                     /locus_tag="VN039_12180"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HWV46753.1"
                     /translation="MAIHSLAGRPAPHSLLVDVEKLLSAYWTKKPDASSREQLVSFGT
                     SGHRGSSFRRSFNENHIVAIAQAVCEYRTTQQTTGPLYLGKDTHALSEPAFVTTLEVL
                     AANGVDIMIDRNGGYTPTPVISHAILTYNRGRTSGLADGIVITPSHNPPEDGGIKYNP
                     SSGGPADTQITKWIEDRANALLAANLQGVERLPIEQARRASTTHQHDYIGAYVNDLNN
                     VVDMNAIKSAKLKLGIDPLGGSGVAYWQPIVEHYGLNLEIVNPAVDPTFRFMPLDWDG
                     KIRMDCSSPYAMANLIALKDRFDVAFGNDADNDRHGIVTRSGLMNPNHYLAVSIAYLF
                     ANRPGWKSDAGIGKTLVSSSLIDRVAAKLNRKLVEVPVGFKWFVSGLLDGSLGFGGEE
                     SAGASFLRQDGTVWSTDKDGIIMDLLAAEMMAKTGRDPSELYRDLTKELGEPVYERID
                     APATPEQKTILSKLSPEQVNATELAGDRIVAMLTKAPGNAAAIGGLKVVTKNGWFAAR
                     PSGTEDVYKLYAESFKGNEHLKRIQEEAQALVAGALGSST"
     gene            1714..>2500
                     /locus_tag="VN039_12185"
     CDS             1714..>2500
                     /locus_tag="VN039_12185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV46754.1"
                     /translation="MNRHALRWPLPKHDYWSTPFAESLLQHLDLRPGLQTLDVASGHG
                     IPAFYLAEQVGPTGEVLAIDISAGQVARARAIQGSQLPWLRFKCMDMRALPADLPTFD
                     RITGNLSVMFFRPNRFEAVRGLAERLNPGGQLVLTFPSYGTFDSLWQRVDLAMIQQGL
                     ITERDRFQAYLNERPSAQEGQSWLERLDLERVEAIEYSLEVKTGPGQEFLHHPLLRGG
                     FLDDVYECFEDQSLAERFMIEISQDVAQFTPLIAQRCVLSGWKR"
BASE COUNT          562 a          723 c          672 g          543 t
ORIGIN      
        1 gggttggccc tttgtagttg acaggatcct gacgggcgaa ctacatctct actgtggcta
       61 ttcatagtct tgccggccga ccagcacctc actccctcct cgtggatgtg gaaaaacttt
      121 tgtccgctta ctggactaag aagccggatg cctcgtcccg agaacagctc gtgtcattcg
      181 gcacatcagg acatcgtggc tcttccttca gacgaagctt caatgagaac catatcgtgg
      241 cgatcgctca agcagtttgc gaataccgaa ctacacagca gacgaccgga cccctttatt
      301 tggggaaaga tacacatgct ctatcagagc ctgccttcgt gacgaccctg gaagtcctcg
      361 cagccaacgg cgtcgacatc atgatcgacc ggaatggtgg atacacgccg acaccagtca
      421 tctctcatgc catcctgact tacaatcgag gccgaacctc cggtctggcc gacgggattg
      481 tcatcacgcc gtcccacaac cctccggaag acggcggcat caagtacaac ccgtcgagcg
      541 gtggccctgc cgatacacag atcacgaagt ggatcgaaga ccgagccaat gcccttctag
      601 ccgccaatct ccaaggagtg gaacgtcttc cgattgaaca ggctcgcagg gcatcgacca
      661 cacatcagca tgactacatt ggagcctacg tgaacgatct gaacaatgtg gtcgacatga
      721 atgccatcaa gtctgcgaaa ctgaagcttg ggatcgaccc cctcggtggg tccggtgttg
      781 cctactggca accgattgtt gagcattacg ggctcaatct tgagattgtg aatccagcgg
      841 tcgatccaac gttccgcttc atgcccttgg attgggacgg caagattcgg atggactgct
      901 cttctcccta tgccatggcg aacctgatcg ccttgaaaga ccgcttcgat gtggcattcg
      961 gtaatgacgc ggacaacgac cgacacggga tcgtcactcg ttccggcttg atgaatccga
     1021 accattacct ggccgtctcg attgcctacc tctttgccaa ccgtcccggt tggaagtccg
     1081 acgcgggcat cggcaagaca ttggtcagca gcagtctcat cgaccgagtg gcggccaaac
     1141 tgaaccgaaa gctggttgaa gtgccggtcg gcttcaagtg gtttgtaagc ggcctgctcg
     1201 atggctcact tggtttcggc ggcgaggaaa gtgccggcgc ctcattctta aggcaggatg
     1261 gcacagtgtg gtccaccgat aaggacggca tcatcatgga tctgctggcc gctgaaatga
     1321 tggccaagac cggccgcgat ccatccgagt tgtaccgaga cctcacgaaa gaattaggcg
     1381 agccggtgta tgaacggatc gacgcacctg ccactcctga acagaaaacc attctctcca
     1441 aactttctcc tgagcaagtg aacgcaacag aactggctgg ggacaggatt gtcgcgatgc
     1501 tcacgaaggc ccccggaaac gccgcagcca tcggtggctt gaaggtggtg accaagaacg
     1561 gctggttcgc ggcaagaccg tccggaaccg aggatgtata taagctctac gcggaaagct
     1621 tcaaagggaa cgagcacttg aagcggattc aagaggaggc acaggccctc gtcgccggcg
     1681 cattgggttc ttccacatga tctgcatggc accatgaaca ggcacgctct ccgctggcct
     1741 ctgccgaaac acgattattg gtccacgccg ttcgctgaat cgttactgca gcacctggac
     1801 cttcgtccag gcctgcagac tctcgatgtc gcttccggcc acggcattcc ggcgttctat
     1861 ctcgctgagc aggttggtcc taccggtgaa gtgctggcca tcgatataag tgccggtcag
     1921 gtcgcccgtg cgagagccat tcaaggttcg caattaccat ggcttcgctt caaatgtatg
     1981 gacatgcgtg ccttgccggc tgatctgccg accttcgatc gcatcacagg caatctctcc
     2041 gtcatgttct ttcgtcccaa tcggttcgaa gccgtccgtg gactcgccga acgactcaac
     2101 cccggcggtc agctcgtcct gacctttccg tcctatggaa cgttcgattc actatggcag
     2161 cgagtagacc ttgcgatgat tcagcaagga ctgatcaccg agcgagaccg attccaggcc
     2221 tatctgaatg aacggccatc ggcgcaagaa ggacaaagtt ggcttgagag actcgatctt
     2281 gaacgagtcg aggccatcga gtattcgctt gaagttaaga ctggtcccgg ccaagagttt
     2341 ctccatcacc cgctccttcg tggcggcttc ctcgacgacg tatacgaatg ttttgaagat
     2401 cagtcgcttg ctgaacggtt catgattgaa atctcacaag acgttgctca atttactccg
     2461 ctgatcgcgc aacgctgtgt gctctcaggg tggaaacgaa
//
LOCUS       DATMTI010000765         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_113095, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000765 DATMTI010000000
VERSION     DATMTI010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113095"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VN039_12190"
     CDS             <1..630
                     /locus_tag="VN039_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46755.1"
                     /translation="GFSGVVERRFNPATFRYALAEQLDLTSRKWSAVDGFVYLCKIHG
                     SISWTEDDHGLFPIREAAASEEPGKVMIYPTPAKQNASLGSPYADLFREFQSRVVREQ
                     SVLFTMGYAFGDEHINNIIYQALTIPTFRLVIFVDPNLEGDIAKLKALNDPRIWIIGG
                     EGPEAGRKAHYFDTIIEQFLPQRPSERIDDAVRKVLEAMAPPPKKEEEE"
     gene            627..>1681
                     /locus_tag="VN039_12195"
     CDS             627..>1681
                     /locus_tag="VN039_12195"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HWV46756.1"
                     /translation="MSRDDRKRAIGKVVSVAADKFVVEMHAGTDNFTVVGFDDVHYVA
                     RLGSFLMIPVQTEYVVAEVVGLRERDVSSSDRRNGDMDKASSAKFLDVVPVGMLPQKP
                     GEKFRFGVSIFPSLYADALYALDAELDRVFETDVPSEPAPKRGELPPIPPEATRFRAL
                     TIGRSVIFDGYDVKVRIDDFFGGHTAVLGNTGSGKSCTVASVLQSLFDKPDEHHARGA
                     TFVVFDVNGEYAQALTPLANGNGIGVSQVVLDGTAAEGRFRLPHWFLDQSEWELLLQA
                     SERTQLPVLRTALGLTSLLVGNDAKRLAIRQHFVATCVVECFRGADGDSPVSKIQRVS
                     ALLEKYGTTALNRGMLS"
BASE COUNT          343 a          453 c          506 g          379 t
ORIGIN      
        1 ggcttttccg gcgttgtgga gcgccgtttc aatccagcga cattccgcta tgccctggcc
       61 gaacaactcg acctgaccag tcgcaaatgg tcggcggtcg atgggttcgt ctatctctgc
      121 aagatccacg gctcgatcag ttggacagag gacgatcatg gcctcttccc gatcagggaa
      181 gccgcggctt cggaagagcc tggcaaggtg atgatctacc ccacgccagc gaagcagaat
      241 gccagcctcg ggtcgccata cgctgacctc ttcagggaat tccagtcgcg cgtcgttcgc
      301 gagcagagcg ttttgttcac gatgggatat gcgttcggtg acgagcacat caacaacatc
      361 atctaccagg cactgacgat cccgacgttc aggctggtga tcttcgtaga ccccaatctt
      421 gagggggata tcgccaagct gaaagctctg aacgatcccc gaatatggat cattggcggc
      481 gaaggcccgg aagcgggacg caaggcgcat tacttcgaca ccattatcga gcagttcctg
      541 ccgcaacggc cgagtgagcg cattgacgac gcagtgcgta aggttctgga ggccatggcg
      601 cctcctccga agaaggagga ggaagaatga gtcgcgatga tcgcaaacgg gcaatcggca
      661 aggtagtctc tgttgctgcc gataaatttg ttgtcgagat gcacgccggc accgacaact
      721 tcaccgtcgt cggcttcgat gatgtgcatt atgtggctcg cctcggttct tttttgatga
      781 tcccggtgca gacggaatac gtcgttgcgg aggtcgtcgg gctacgcgag cgtgatgtca
      841 gcagttctga tcgacgcaat ggcgatatgg acaaggcgtc atcggcgaag tttctggacg
      901 tcgtgcctgt tgggatgctg cctcaaaagc cgggagagaa atttcgcttt ggggtgtcca
      961 tatttccatc gctctacgcg gacgcgcttt atgctctgga cgcagagctc gatcgcgtgt
     1021 tcgaaacgga tgttccgagc gagcctgcac ccaagagagg cgagctgccg cctattcctc
     1081 cagaggcaac ccggttccgc gccttgacga tcggccggtc tgtcattttt gacggctacg
     1141 atgtcaaggt tcggatcgat gactttttcg gagggcacac ggccgtcctc ggaaatacag
     1201 gcagcggcaa gtcctgcacg gttgcgtccg tgctgcagtc gctgttcgac aagcccgacg
     1261 agcatcatgc ccgtggcgcg acgttcgtag ttttcgacgt caacggagaa tatgcccagg
     1321 cgctaacacc cttggcgaac ggcaacggga tcggtgtttc tcaagttgtt ctcgatggaa
     1381 ccgctgccga aggccgtttt cggttgccgc attggttcct tgatcagtcg gaatgggaac
     1441 ttttgttgca ggccagtgaa aggacgcagc ttcccgtttt gaggacagcg ttgggtctta
     1501 ccagcctttt ggttggaaat gatgcgaaac gtttggccat aaggcagcac ttcgtagcga
     1561 cttgcgttgt ggagtgcttc agaggcgcag atggcgattc ccctgtctct aagatccagc
     1621 gtgtgtcggc gctcctcgaa aaatacggga ccacagctct caatcgcggc atgctgtcga
     1681 a
//
LOCUS       DATMTI010000766         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_199682, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000766 DATMTI010000000
VERSION     DATMTI010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199682"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..2927)
                     /locus_tag="VN039_12200"
     CDS             complement(36..2927)
                     /locus_tag="VN039_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46757.1"
                     /translation="MMKPSGRKRTRNRRRLAASLLASSTGPELLEGLPLAVVILDNDF
                     AILAVNRESHRLLGPRFSSSMIRSFPSLWSMLTQSDTTTMTAQLNEVLKSRRPTSVTQ
                     HLLLRGATRPVPVEWTCAPGTFDGKTVLIISIRDLSHELELEQDRDRLAAIAEESPLP
                     MIELDRQMSLLYANPAMISLLTRFGYSLEGFPNVAPPSLPDIVRRCLATGHVFHDETV
                     VLPEASFSWTFCPILTHGLVRGYATDMTSVHKTQQALQLSADQLRRSNCRLDQALDEA
                     KESARVKTAFLATVSHELRTPMNGVIGMTSLLMETSLTSEQQSYAETIRQCGEALLHL
                     INDVLECSKIEAGKLELECLDFNLRTIVEQVLAQFAERAETKGLELTGLVHASVPTGL
                     KGDPGRLRQILTNLVGNAVKFTDKGEVILQAYLEEDLPDTAVIRFEVTDSGIGINPNT
                     QSKLFRPFVQADSSTTRKYGGTGLGLSISKQLVELMDGRIGVHSTEGQGSTFWCTARF
                     QKQADSLRAILPTGDLTGKRVLIVDDNESNRLILHHLVSGWGMVDDLAEDAESALHRV
                     AKAKRRGRPYDLAILDVIMPGKDGLQLARELQSHPAGSGIRLVVMTSMLQRGHAEQAR
                     QAGAMGYLPKPVRHDELRDCLRTVLGLPAGEAPKDAQTCPVVPQLVTRHMVAEHVQHL
                     RILVVEDNIVNQKLAVRMVEKLGYKPDVVENGKEALTALTKGDYAAILMDCQMPVMDG
                     FEATRCIRERETSVSSHDSPDGSASRSDRAPQSTPHMPIIAVTANAMQGDRERCLAAG
                     MDDYLAKPIKLEELRNALARWVSAPPEVVVPGKQQPISTTADPTRGIFDPAKMYQNIG
                     GDSELFAQLVRLFLDRHHTMLAGIRTALAEADSIAVERIAHTFKGTAGNLCASQVALT
                     AGRLEAVGRLNALHDAPPVYAQLELEVARLVRVLESYREGYQPVTEAAA"
     gene            complement(2924..>4178)
                     /locus_tag="VN039_12205"
     CDS             complement(2924..>4178)
                     /locus_tag="VN039_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HWV46758.1"
                     /translation="TKRIAIEQLTPGMFIIEMDVPWYRTPFLSHKRLIKDLETIQLMK
                     QHGIKMVTIDTSKGSDLPPAPSTNAQHVASQHSTNPDVPAPPEQKTERASAAKSDDHS
                     ATMIYAQAQEAVERIFDDLEQGIPPSPEAAKVIVSNVLKQILSDRTAMATQVAIHKIK
                     QFDRSLTAHALDTCILSLIVAIESGLDQSTQEQVGMGALLHDAGYVRLPRNLVRKREE
                     CSGQDKTLLEQHCKLGVALLSEHPGMHEDVLRIVREHHERADGSGFPTGLGSDTIAHL
                     AQIVGIVDVYDSMVSRRGTRPAMIPHDAVRQLFLAAERGQFEKSLVELMIRSIGVYPV
                     GSLIQLNTGEQAVVVGVNPQQRLKPLVKITTDPHGGSYATPIEVDLAIPSPDHKIRTV
                     LRVLDPAKERVNIGIHLNQTLSRAA"
BASE COUNT          804 a         1140 c         1270 g          964 t
ORIGIN      
        1 cagcagtttg acgacttcct gctagaccac gagaatcagg cggctgcctc ggtgacgggc
       61 tggtatcctt ctcggtagga ttcaagtaca cggacgagtc gggcgacttc gagttcgagc
      121 tgcgcgtaga caggtggggc gtcgtggagt gcgttgaggc ggccgactgc ttcgagacga
      181 ccagcggtga gtgccacttg ggaggcgcaa agatttccgg cggtgccttt gaaagtgtgt
      241 gctattcgct cgacggcgat cgagtcggcc tcggccagag ccgttctgat gccggcgagc
      301 atggtgtggt gccgatcgag aaacaggcgg acgagttgag cgaacaactc actgtcgccg
      361 ccgatgttct ggtacatttt tgcaggatca aagattccgc gggttgggtc ggccgtcgtg
      421 gaaattggtt gctgtttccc ggggaccacc acctcgggcg gcgcggatac ccaacgtgcc
      481 aaggcgttcc ttagttcctc tagtttaatc ggtttggcca gataatcgtc cattccggcc
      541 gccaagcagc gttcgcgatc gccctgcatc gcattggcgg tgactgcaat aatcggcatg
      601 tgcggagtcg attggggagc cctgtctgag cggctcgcgc ttccatcagg cgagtcatgt
      661 gatgacaccg acgtttcacg ttcccgaatg catcgggttg cctcaaatcc gtccatgacg
      721 ggcatctgac aatccatcag gatggccgca taatcgcctt tggtaagcgc ggtcagcgcc
      781 tctttgccgt tttcgacgac gtccggtttg taacccagtt tttcgaccat ccgcacggcg
      841 agcttctggt tgacgatgtt gtcttccacg accaaaatgc gcagatgttg cacatgctcc
      901 gcgaccatat gcctggtgac gagttgaggc acgacggggc atgtctgggc gtccttcggc
      961 gcttcgcccg ccggcaagcc gagcacggtt cgtagacaat cgcgtaattc atcgtgacgg
     1021 acgggttttg gtagatagcc catcgcgccg gcttgacggg cttgttccgc atggccccgt
     1081 tgcagcatcg aggtcatcac aacgaggcga attcctgatc ctgccgggtg gctttgcagt
     1141 tctcgcgcaa gctgcaaccc gtcctttccc ggcatgatga cgtccaagat cgccagatcg
     1201 tacggccttc ccctccgctt tgcttttgcg acgcggtgta aagccgattc ggcatcctct
     1261 gcgagatcgt caaccatccc ccaacccgat accagatggt ggaggatcaa acgattcgat
     1321 tcgttatcat cgacgatcaa gacacgcttt ccggtgagat ctccggtcgg aagaatcgca
     1381 cggagagaat ccgcctgttt ctggaagcgc gcggtacacc agaaggtgct gccctgtccc
     1441 tccgtgctgt gcactccaat tcgtccatcc atcaattcca cgagttgttt ggaaattgaa
     1501 aggccaaggc cggttccgcc gtattttctc gtcgtggagc tgtcggcctg cacaaacgga
     1561 cggaacagtt tggactgggt attcggattg attccaatgc cgctatccgt gacttcgaaa
     1621 cggatgacgg cagtgtcggg taggtcttct tcgagatagg cctgcaggat cacctcaccc
     1681 ttgtcggtaa acttcaccgc attgccgacc agattggtga ggatctgacg cagacggcct
     1741 gggtcgcctt tgagtcccgt cggaaccgac gcatggacaa gtccggtcag ttccagccct
     1801 ttcgtctccg cccgttctgc gaattgtgcc aacacttgtt ccactatggt cctgagattg
     1861 aagtcgaggc actccagttc cagcttgccc gcctcgatct tgctgcattc gagcacgtcg
     1921 ttgatgaggt gcagcagcgc ttcgccgcat tgccgaatag tttcggcgta ggactgttgt
     1981 tcggacgtca aggaggtttc catcaacagg ctcgtcatac cgatcacgcc gttcataggg
     2041 gtacgcagtt catgactgac ggtcgctaga aatgccgtct tgacgcgtgc cgattccttg
     2101 gcctcgtcca gagcttggtc caaacgacag ttactccggc gaagttggtc ggcggagagt
     2161 tggagcgcct gctgtgtctt gtggacgctt gtcatgtcgg tggcatagcc tcgcacgagc
     2221 ccatgagtga gaatcgggca gaaagtccat gaaaaactgg cttctgggag cacaaccgtc
     2281 tcatcatgaa acacgtggcc tgttgcgaga cagcgccgca cgatgtccgg aaggctaggc
     2341 ggggcgacgt tgggaaatcc ttcgaggctg tacccgaatc gagtcagcag agagatcatc
     2401 gctggattgg catacagcag gctcatctgc cggtccagtt cgatcatcgg caaagggctt
     2461 tcctcagcga tcgcggccag ccgatcgcga tcttgctcca actccagttc gtgggacaag
     2521 tctcgaatgc tgataatcag cacggtcttt ccatcaaatg taccgggcgc gcaagtccat
     2581 tccaccggaa cggggcgggt tgctcctcgc aagagaagat gttgcgtcac agaagtcgga
     2641 cggcggcttt tcagcacttc gttcagttgc gctgtcatgg tggttgtgtc ggactgggtg
     2701 agcatcgacc acagggatgg aaaggatcgg atcatggaag aagaaaaccg tggtccaagc
     2761 aaccgatggc tctcacggtt aacggccaat atggcgaaat cattgtcgag gatgacgacg
     2821 gccagcggaa gcccttcgag gagctcgggg cccgtggaag acgccagaag cgaggctgca
     2881 agtcgacggc ggttgcgtgt gcgtttccgt ccagacggct tcatcatgcg gctcgtgaga
     2941 gggtttgatt caggtgtatc ccgatgttga cacgttcttt ggccgggtct aagacgcgca
     3001 gcacggttcg gatcttgtgg tcgggagatg gtatcgcgag gtccacctcg atgggcgtgg
     3061 cataagagcc tccatgcggg tcggtcgtga ttttgaccag gggtttgagt cgttgttgcg
     3121 gattgactcc gacgacgacc gcctgttcgc cggtattgag ctggatgagg ctcccgaccg
     3181 ggtagacgcc gatgctccga atcatgagct ccacgagaga tttttcgaac tgtcctcgtt
     3241 ccgcggccaa gaagagttgc cgaacagcgt cgtgcggaat catagccgga cgcgtcccgc
     3301 gccgactgac catgctgtca taaacatcga cgatgccgac aatctgagcg agatgcgcga
     3361 ttgtgtcgct tcctagaccg gttggaaacc cgcttccatc ggcccgctca tgatgctccc
     3421 tgacgatgcg taggacatcc tcgtgcatgc cgggatgctc ggagaggagt gcgacgccta
     3481 gcttgcaatg ctgttcgaga agggtcttat cttgtccgct gcattcttcc cgcttacgca
     3541 cgagattgcg cgggaggcgg acatacccag cgtcatggag caatgcgccc atgccgacct
     3601 gttcttgcgt cgattggtcc aacccgctct caatggccac tatgagtgac aagatgcatg
     3661 tgtccagtgc atgagccgtc agggagcggt cgaactgctt aattttgtga atggcgactt
     3721 gagtggccat tgcggtgcga tcgctgagaa tttgtttcaa cacgttcgac acgatgactt
     3781 tggccgcttc aggggacggc gggattccct gttccaggtc gtcgaagatt cgctcaaccg
     3841 cttcttgagc ctgcgcatag atcatggtgg cggaatgatc gtcagatttc gccgcagacg
     3901 cgcgttcggt cttttgctca ggaggagccg ggacgtccgg gttggtcgag tgttggctcg
     3961 caacgtgctg cgcattcgtt gaaggggccg gcgggaggtc ggaccctttg ctggtatcga
     4021 tggtcaccat cttgatccca tgctgcttca tgagttggat ggtctccagg tccttgatca
     4081 gccgtttatg ggagagaaac ggagttcggt accacgggac atccatctct atgatgaaca
     4141 tgccgggagt gagctgttcg atcgcgatac gctttgtc
//
LOCUS       DATMTI010000767         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_622740, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000767 DATMTI010000000
VERSION     DATMTI010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622740"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VN039_12210"
     CDS             <1..693
                     /locus_tag="VN039_12210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWV46759.1"
                     /translation="ATRTRPRTDGEEGLRVLSVLQRCQEALEQAGPRMTPPVPKPDKP
                     YFAHESAFVDEGVDVGAGTSIWHTSHILKGSRIGKNCKIGQNVVVGPNVTVGNGVKIQ
                     NNVSVYEGVTLEDHVFCGPSMVFTNVFNPRSEIPRMKELKQTLVKRGATLGANSTILC
                     GITIGRYAFIGAGTVVTKDVPDHALVVGNPSRVTGWMCLCGVKLHVKGENAACPACGQ
                     QYRAEQAGMTVV"
     gene            708..>1560
                     /locus_tag="VN039_12215"
     CDS             708..>1560
                     /locus_tag="VN039_12215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWV46760.1"
                     /translation="MGVPLLDLKAHHEPIHHEIMAALEQTFRSQAFILGPDVGKLEER
                     VATYCQAKYGIGVSSGTDALLIALMTLGIGPGDEVITTPYSFFATAGAVVRLGAKPVL
                     VDIDPVTYNIDPAKIKSVLTAKSRAIIPVHLYGQCADMAPIMDIAKRHNLSVIEDAAQ
                     AIGSEYHDGRRACSIGTIGCLSFFPSKNLGCLGDGGMVVTSDADLAERMRVLRVHGSK
                     PKYYHKRVGGNIRLDTIQAAVLNVKLNYLDGWTKRRQENATRYETLFKQSGLTQTGKV
                     KLPEPVYR"
BASE COUNT          366 a          378 c          470 g          346 t
ORIGIN      
        1 gcaacgcgga ctaggccaag gacggacggg gaggaagggt tgcgtgtgct atccgtgttg
       61 cagcggtgcc aagaggcatt ggaacaggcc ggtccgcgca tgacgcctcc tgtccccaag
      121 ccagataagc cttacttcgc ccacgaatcg gccttcgtcg acgaaggcgt ggacgtcggt
      181 gcgggaacga gcatttggca cacatcacac attctgaaag ggtcgcgcat cgggaagaac
      241 tgtaaaatcg ggcagaacgt cgtcgtcggc cccaatgtta cagttggcaa tggagtcaaa
      301 attcagaaca acgtatccgt ctatgaaggt gtaacgcttg aagaccatgt cttctgtgga
      361 ccttcgatgg tttttacaaa tgtctttaat ccccggagcg agatccctcg tatgaaagag
      421 ctcaagcaaa cgcttgtaaa acgaggagct acgctggggg ccaattcgac gattctgtgt
      481 ggaattacga tcgggcgata tgcgttcatc ggtgcgggaa cggtcgtgac caaggatgtg
      541 ccggatcatg cactggtagt cggcaatccc agccgggtta ctggctggat gtgtctgtgt
      601 ggggttaagc tccatgtcaa gggcgagaat gccgcctgtc cagcctgtgg gcagcaatat
      661 cgggccgaac aagcggggat gacggtggtt taggaaagga gatcgtcatg ggtgttccat
      721 tacttgattt gaaagcacat cacgagccga tccatcatga aatcatggcg gcgctggaac
      781 agactttccg tagtcaggcc tttatcctcg ggccggatgt aggcaagtta gaggaacgtg
      841 tggccaccta ttgccaggct aagtacggta taggtgtgag ttctgggacc gacgctctcc
      901 tgatcgctct catgactctc ggtattggtc ccggtgatga ggtcattacg acgccctatt
      961 cgttctttgc cactgccggg gcggtcgtga gactgggagc caagccggtg cttgtagaca
     1021 tcgatcccgt aacctacaac atcgatcccg ccaaaattaa gtcggtgctg acggctaaga
     1081 gcagggctat tattccggtc catctctatg gacaatgtgc cgacatggcc ccaattatgg
     1141 atatcgccaa acggcacaat ctaagcgtca tcgaggatgc ggcccaagcc attggttcgg
     1201 aataccatga tggacggcga gcctgtagta tcgggactat cggttgcctg tcgtttttcc
     1261 ctagtaagaa tctcggatgt ttaggcgatg gggggatggt tgtgactagc gatgctgacc
     1321 ttgcagagcg catgcgggtc ctgcgagttc acggaagcaa gccgaagtat tatcacaagc
     1381 gggttggagg gaacatccgt ctcgatacga ttcaggccgc ggttctgaac gtaaagctca
     1441 attatctcga tgggtggacc aaaagacggc aggagaatgc cactcgatac gagactctgt
     1501 tcaaacagag cgggctcacg cagactggga aagtgaaatt gccggagcct gtataccgca
//
LOCUS       DATMTI010000768         1791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_156189, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000768 DATMTI010000000
VERSION     DATMTI010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156189"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VN039_12220"
     CDS             complement(<1..460)
                     /locus_tag="VN039_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46761.1"
                     /translation="MNQLIRSLVLAVLFPLAAGCVNVVNPALVKVTYDKPITTTSDKT
                     ARLELITGAVQGDSPYPSSLFNAQDQQVFTESLKEELLRLNLLRVLREGESTIKGTDI
                     LILLMFARTTHDRNLHDYQLDVAMEIRFADRAFKKRYQVLSSEGESIFIRM"
     gene            819..1025
                     /locus_tag="VN039_12225"
     CDS             819..1025
                     /locus_tag="VN039_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46762.1"
                     /translation="MTSVHEQAFLEKLRHLPPEQVVEVEHFVDFLVHRQAEEHRLTQV
                     SSQVAEPAFARIWDNPDDADYDRL"
     gene            1012..1338
                     /locus_tag="VN039_12230"
     CDS             1012..1338
                     /locus_tag="VN039_12230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWV46763.1"
                     /translation="MTGYEFGQIVLVPFPFTDQSATKHRPAVVISSTAYHRVRPDLII
                     MAVTSQQPTAEVVVEDWRAAGLLKPSVLKPVLTTIDPTLILKKLGRLTTSDQVHLRQA
                     LAQIIG"
     gene            complement(1406..>1791)
                     /locus_tag="VN039_12235"
     CDS             complement(1406..>1791)
                     /locus_tag="VN039_12235"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV46764.1"
                     /translation="ADRITLRCERYEEVPGARVVDAAISNSLLHHLSNPLQFWQKLRQ
                     LVKPGAPVLVMDLLRPESSEAAQAIVDQYAANEPDILRRDFYNSLLAAFTEDEIGSQL
                     ARMNLTRLLIDIPDDRHWVVGGIIY"
BASE COUNT          449 a          510 c          418 g          414 t
ORIGIN      
        1 ccatacgtat aaatatgctc tccccctctg acgatagaac ttgataacgc ttcttgaatg
       61 ctctatcggc aaagcggatt tccatagcaa catctaactg atagtcgtgt agatttcggt
      121 cgtgcgtcgt acgcgcgaac atcagcagaa tcaggatatc cgtacccttg atcgtcgact
      181 ctccctctct caatacacgc aagaggttga ggcggaggag ttcttctttg agagactccg
      241 tgaatacttg ctggtcttgc gcattgaaca acgaactagg gtaagggctg tctccttgta
      301 cagccccggt aattagttcc agccttgcag ttttgtcgga tgtcgttgta ataggcttgt
      361 cataggttac ttttaccaag gccggattga ccacattcac acaaccagcg gccagcggaa
      421 acaagaccgc taaaactaga cttcgaatca gctggttcat ttgtcctcta tcccgcctag
      481 accttatcaa cagataccca acaatgatta gacagactgt ataatccagc agagacaacc
      541 tgtcccgcgc ctgcctttag ctggatgctg aaaatacgct aaaacataga ctcgctatcg
      601 ctcatcaata aatgacccct cccaccaccc agtgccggtc gtctggaata tcgatcagca
      661 gcctcgtgag attcatgctg gccagctgct aaccgatgtc atcctccgtg aagacagccc
      721 gtccaaaatc atgatcaacg cctgagaaga ggttccgcgt ctgcaattga tctttggcaa
      781 cactcgttgt aaggtaacgg ctggcaggat aaatgatcat gacgagcgta cacgaacaag
      841 cgttcctcga aaagcttcgc catcttcccc cggaacaggt ggtagaagta gagcattttg
      901 tcgacttcct agtccatcgc caagccgaag aacatcgatt gacccaggtt tccagccaag
      961 tcgcggaacc tgccttcgcc cgtatttggg ataatcccga cgacgccgac tatgaccggc
     1021 tatgagttcg ggcagatcgt cctcgtcccg tttcccttca ccgatcaatc cgccacgaag
     1081 caccgcccag ccgtagtcat cagcagtacc gcctatcatc gggtgcgccc cgacctgatc
     1141 atcatggcag tcacgagtca gcagccaacc gcggaagtgg tggtcgaaga ctggcgggca
     1201 gctggactgc tcaagccgtc tgtgctcaaa ccagtcctga cgacgattga tccgacgcta
     1261 attctgaaga agctcggacg acttaccact tcagatcaag tgcacctgcg acaggcactt
     1321 gcacaaatta tcggctaatc tcttcggcaa cgaggcgcac cattgaagat ggactaacag
     1381 tcctggtcct ggttccccgt ccatctcaat aaatgatccc tcccaccacc caatgtcgat
     1441 catctggaat atcgatcaac agcctggtga gattcatgcg ggccagctgt gaaccgatct
     1501 catcttctgt aaaggccgcg aggagagaat tatagaaatc ccggcgaaga atgtccggct
     1561 cgttggccgc atactgatca acgatggctt gtgccgcttc agacgattcc ggacgcagca
     1621 gatccatcac cagtactggt gccccgggct tcaccagttg tcgaagtttt tgccaaaact
     1681 gcaacggatt ggacaagtga tggagcaggc tattggaaat cgctgcatca acgactcttg
     1741 cgccaggcac ctcttcgtat cgttcgcatc gtaatgtaat acgatcagcc a
//
LOCUS       DATMTI010000769         1518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_706864, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000769 DATMTI010000000
VERSION     DATMTI010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706864"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..139
                     /locus_tag="VN039_12240"
     tRNA            62..139
                     /locus_tag="VN039_12240"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Glu,seq:ttc)
     gene            complement(344..1345)
                     /locus_tag="VN039_12245"
     CDS             complement(344..1345)
                     /locus_tag="VN039_12245"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HWV46765.1"
                     /translation="MDERMRFVIRLKDGESMAGLCREFGISRKTGYKIFERYEQGGLE
                     GLSDRARRPFRYANQLPEPVEAAIVAAKREKPHWGARKIRERLLRRLPHAIKVPAAST
                     VHAVLDRHGLVKRMSRARTRAEGTPLSDGLQPNDLWCTDYKGEFQLGDKRYCYPLTVT
                     DHASRFLLLCEALESNREELALRAFERLFRERGLPLAIRSDNGVPFASPHALFQLSRL
                     SVWWLRLGISIERIRPGHPQQNGRHERMHLTLKKEATRPAGNNILQQQGKFDIFLEEF
                     NNERPHEALNMQCPAQLYTASPRDFTWAFPSRTTRFTIKPCWSPAAAASAFIARKSI"
BASE COUNT          320 a          434 c          428 g          336 t
ORIGIN      
        1 cggacggatc gcttctcacg gtacagtgtg atttggaacg tttaccgttc catcctgctg
       61 cgtccccatc gtctagcccg gcctaggaca ccgccctttc acggcgataa caggggttcg
      121 aatccccttg gggacgccaa atcaatcata tccggaccga acacataggt aacacttcgt
      181 tcctaagaca taggtaacac ttttggcccg aagggatttt cgaggggctg caaagttttc
      241 tcctccaaat cgatatagcc gaggtcgtaa tgcataaagc tgacgagcca gatgccgtcc
      301 tcgacttctt tgatgccgac ggcctgaccc gccagcgatt tgctcagatt gattttcttg
      361 cgataaaggc agatgcggcc gcagcaggtg accaacacgg ttttatcgtg aaacgggtag
      421 tgcggctcgg gaatgcccac gtaaagtctc tgggggaagc ggtgtagagc tgtgccgggc
      481 attgcatgtt gagcgcttcg tgaggacgtt cgttgttaaa ttcttctaag aagatgtcga
      541 acttgccttg ttgttggaga atattgtttc ctgccgggcg ggtcgcttcc ttcttgagcg
      601 tgaggtgcat gcgttcgtgc cggccgttct gctgcggatg accaggccgg atacgctcaa
      661 tcgagatccc caatcgcagc caccagaccg aaagcctgga gagctgaaac aaggcatgcg
      721 gagaagcaaa cggaacgccg ttgtcggaac gaatggccag cggtaaacca cgctcgcgaa
      781 acagcctttc aaaggcgcgt aaggccagtt cttcgcgatt ggattcgagc gcttcgcaca
      841 gcaggagaaa gcgcgaagca tggtcggtga cggtgagcgg ataacaatag cgtttgtcac
      901 cgagttggaa ctcgcccttg tagtcggtgc accacaaatc gttcggctgc aaaccatcgg
      961 agagcggtgt gccttcggct cgcgtgcgcg cccggctcat tcgcttcacc aggccatggc
     1021 gatccagcac cgcatgtacg gtactggcgg cgggcacttt gatggcgtgc ggcaaacgtc
     1081 gtagcagacg ctcacggatc ttgcgtgctc cccagtgcgg cttctcccgt ttggcggcca
     1141 cgatggcggc ctcgactggc tcgggcagtt ggttcgcata gcggaacggc cggcgcgcgc
     1201 gatcactgag cccctccaga ccgccttgtt cgtaccgctc gaagatcttg tagccggtct
     1261 tgcgcgagat accaaactcg cggcatagcc cagccatgct ttcgccgtcc ttgagtcgaa
     1321 tcacaaaccg cattcgttcg tccataacag agctttcctt ccatggcatc cacctcaccc
     1381 gccccctgcg ggacgaaaaa gtgtaaccca tgtctccgga acgttctgtt acctatgtct
     1441 cgggtcggtc agtggctcta actaaccgat agctatagac caacaattta ggtcgagaac
     1501 ggcatccacc atctgcat
//
LOCUS       DATMTI010000770         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_326505, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000770 DATMTI010000000
VERSION     DATMTI010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326505"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VN039_12250"
     CDS             complement(<1..382)
                     /locus_tag="VN039_12250"
                     /inference="COORDINATES: protein motif:HMM:NF021584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HWV46766.1"
                     /translation="MQRLVVLSVVLLAFLISPAAFPQSFTDKAKKDSAVDMSDEDPAM
                     QKAMERARTGLEDFLRKAGSPPTNTDQYSVKVRVSEGDKQEYLWVSNLKAQGDLWSGR
                     IDNLPIIRSVKKGQSYSFAKTEIVD"
     gene            619..1425
                     /locus_tag="VN039_12255"
     CDS             619..1425
                     /locus_tag="VN039_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWV46767.1"
                     /translation="MARFAHRRWQNLCLFLRAVGRTPPATRLGISAALLFIVLLGINW
                     VYHTFYKPTELFFPVENALTKSPRETWREYGALFEVHSTAIITPELLAALAQAEGSGN
                     PVARTYWRWRFVSSNPLEWYQPASTAVGMLQITDGTFQEGKRYCIHNHVVVEDGPWHN
                     FNSCWFNSLYTRVIPGHAIELTAAYLDRHVAKAVGERPATFQQKQDLAAIIHLCGAGA
                     GRDYAKRTFRLLANQRCGDHDVKAYLTRVRAMQRSFAMLAAGSIPTSIHR"
     gene            1515..2999
                     /locus_tag="VN039_12260"
     CDS             1515..2999
                     /locus_tag="VN039_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HWV46768.1"
                     /translation="MDRLLSVALALTLLTPSIGSADGSILQSKESQTGELIGPTLPDQ
                     ETHGHHTTFNSGSTVLNWETGAGRSYLIPAGELLAYLFLLNQYDRHFTDPKSAYRTDG
                     DTIWQHLTDSKWVLDNDQFSVNQFLHPYGGSIYYGIARSTGLSFWESWLYSSAGSFFW
                     EIAGETSSPSINDMIATPIGGTLLGEPLFRMASLLLETDEGKPGFWRELGAAVLSPPT
                     GFNRLMFGNRFDTVFPSRQPATFLRLRVGGIVSTSSHNVPSGVREHGAIGDVTFTYGL
                     PGKPGYRYARPFDYFDFHVTAVTTNTLESINTRGLLIGTTYALGEPTRGAWGLFGSYD
                     YISPQVFRASSVALSLGTIWQSWLSRSVALQGVALAGPGYGAAGSIQRTEERDYHYGT
                     TGQGLLALRLIVGDRAMLDFTGREYYVSGLLSAEPHGQENILRGETSLTFRIFGPHGV
                     ALRYAVSHRDARYPNVEFRDQTVETISLMYVFLGKTGFGAVEWR"
     gene            3090..>3349
                     /locus_tag="VN039_12265"
     CDS             3090..>3349
                     /locus_tag="VN039_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV46769.1"
                     /translation="MGSSGPKALWKTVLAGAIGNVLEWYDFALFGYFAPVLSHLFFPA
                     SDPSLSLIATFAVFAVGFLARPLGALLFGYWGDTRGRRAALA"
BASE COUNT          671 a          970 c          905 g          803 t
ORIGIN      
        1 aatcgacgat ctccgtcttg gcgaatgagt atgactgtcc cttcttgact gaacggatta
       61 tcgggaggtt gtcgatccgt ccggaccaga gatctccttg tgctttcagg ttggagaccc
      121 acagatattc ttgcttgtcg ccttcactca ctctgacctt gaccgaatac tggtcggtgt
      181 tggtcggcgg cgatccggct tttcgaagaa aatcttccaa gcccgtccgg gcacgttcca
      241 tggctttctg catcgcaggg tcctcgtcac tcatgtcgac ggcgctgtcc ttcttggcct
      301 tgtcggtgaa tgattgcggg aacgcggcgg gggatattag gaacgccaac aggacgacgc
      361 tcagcacgac gagtcgttgc ataccaacct cccagagtct cctactcacc gtgaatgatg
      421 tgaatattgt cgtttggcca tattgagcct atccggaccg caatgcaaga tcgttcacac
      481 attcagcgtc tcacagaacc cttgcgtaca tccatcacat aatctcagga agaatttatt
      541 tgttttctgt tactctccaa catgcgccgt cgccgccata cccgttcccg ctctcgtcat
      601 cctcatccag taatgtcgat ggcgcgcttc gctcatcggc gttggcagaa tctctgcttg
      661 tttctccggg ccgtcggtcg aacgccaccc gcgacgaggc tgggaatcag tgccgccctt
      721 ctcttcatcg tcctgttggg tatcaattgg gtgtatcaca cgttctacaa gccgacggaa
      781 ttgttctttc ccgtggagaa tgcgctcacc aagagtccac gagagacatg gcgagaatac
      841 ggcgcgttgt tcgaggtaca ttcgacagcc attattacac ccgaactgct cgccgcgttg
      901 gcccaagctg aaggctccgg aaatccagtg gcacgaacct actggcgctg gcgtttcgtc
      961 tcgtcgaatc ccctagagtg gtaccaaccg gcctcgacgg cagtcggcat gctccaaatc
     1021 accgacggca cgtttcaaga agggaaacgc tattgcatcc acaaccatgt ggtcgtcgag
     1081 gacgggccgt ggcacaactt caactcctgt tggttcaata gcctctatac cagagttatc
     1141 ccaggccacg cgatcgaact gaccgctgcg tatctcgacc gtcacgtagc gaaggcggtc
     1201 ggagaacgac cggcgacgtt tcaacagaag caggacctgg ctgcgatcat ccacctctgc
     1261 ggtgccgggg caggccgtga ctatgcaaag cggacctttc gtctcctggc caaccagcgc
     1321 tgcggtgacc atgatgtgaa agcctatctc acgagagtca gggccatgca acgtagcttt
     1381 gcgatgttgg cggccgggtc gattcccacc agtatccatc gttgagcccg accaggatgg
     1441 aaccaagacg accttcagcc ctaggggggt gccgagtatt aatagaacct agtgtattat
     1501 ccactcgtag acgcatggat cgtttattat cagtcgccct ggcattgaca ctcctgacgc
     1561 caagtattgg ttcggctgat ggctccatct tgcaatccaa ggaaagccaa acaggtgaac
     1621 tgatcggtcc gacccttccc gatcaagaga cgcatggcca tcacacgact ttcaacagtg
     1681 gaagcacggt cctcaattgg gaaaccggtg ccggtcgcag ttatcttatt cccgcaggcg
     1741 agcttttggc ctacctcttt ctcctcaatc aatacgaccg acattttact gaccccaaga
     1801 gcgcctatcg cacggacggc gataccattt ggcaacatct cacggattcc aaatgggttc
     1861 tcgacaatga tcagttttcc gtcaatcaat ttctgcatcc gtacggcggc agcatttatt
     1921 atggaatcgc tcgatcgaca ggcctcagtt tctgggaatc gtggctctat agttctgccg
     1981 ggagcttctt ttgggaaatt gcgggcgaga catcgtcacc atcgatcaac gacatgatcg
     2041 ccactcctat tggtggcacc ttgctgggag aaccgctctt tcgcatggca agtctgttgc
     2101 tcgaaaccga cgagggaaag cccggcttct ggcgagaact gggcgcagca gtcctctcac
     2161 cccctacggg gttcaaccgc ctgatgttcg gtaaccgttt cgatacggtc ttcccgagcc
     2221 gccaaccggc cacgttcttg cgtctgcgag tcggcggcat cgtctcaacg agcagtcaca
     2281 acgttccatc gggcgttcgg gagcacggcg ctattggaga tgtcacgttt acgtacgggt
     2341 tgcccggaaa gccgggctat cgttatgcac ggccgttcga ctattttgac tttcatgtta
     2401 cagcggtcac gaccaatacg ctggaaagca tcaatacacg cggcttgttg atcggaacca
     2461 cctatgcgct tggtgagccg acccgtggag cgtggggttt gttcggcagt tatgactata
     2521 tttccccgca agtgttccga gcctccagcg tcgcgctttc gctcggcacc atctggcaat
     2581 cctggctttc acgttcggtt gctcttcaag gcgtggcgct tgccggtccc ggctatggcg
     2641 cagccggcag cattcagcgt accgaagaac gtgactacca ctatgggacg acaggtcaag
     2701 gactcctcgc cctgcggctg atcgtcgggg atcgtgcgat gctcgacttc accggacgtg
     2761 aatactatgt cagcggtctt ctgtccgcag agccgcatgg acaagagaac attttgcgcg
     2821 gtgaaacatc cttgacgttc cggattttcg gcccacacgg tgtggcgctc cgctatgccg
     2881 tctcgcaccg cgatgcccgc tatcccaacg tcgaattccg cgatcagacc gtcgaaacca
     2941 tcagcctcat gtatgtcttt ctcggcaaga caggtttcgg cgcggtggag tggcggtgag
     3001 tgagatggta caaggcacag ccaggacact ccagcgtcca tcatggtatg gtggccgcaa
     3061 ctttgcgctg ttgtatgagg ggcggcttca tgggttcaag cggaccgaaa gcattatgga
     3121 agacggtctt ggccggcgcg atcgggaatg tgctggagtg gtatgacttt gccctctttg
     3181 gatacttcgc cccggtcttg tctcatctct tcttccctgc ttcggatccg tcattatcat
     3241 taattgccac gttcgccgtc ttcgccgtcg gctttcttgc cagaccattg ggtgcgctgc
     3301 tgttcggata ttggggtgat accagggggc gacgagcggc cttggcctg
//
LOCUS       DATMTI010000771         4277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_242488, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000771 DATMTI010000000
VERSION     DATMTI010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242488"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VN039_12270"
     CDS             <1..417
                     /locus_tag="VN039_12270"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HWV46770.1"
                     /translation="AVVDPFDYPNLEDLREEIISIIDDHLSRMSTIPRVVFGEPFHFK
                     RSRILEVPIGLEARSLAEFRQVVAEVDVSAIYFHMFEAHFRLGREESDFSAWIRTGLG
                     LHELADRIRSINPYLGSLERLRSSLITVCDEFLARG"
     gene            427..2106
                     /gene="treS"
                     /locus_tag="VN039_12275"
     CDS             427..2106
                     /gene="treS"
                     /locus_tag="VN039_12275"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HWV46771.1"
                     /translation="MLNQDPLWYKDAVFYELHVRAFYDSNDDGIGDFAGLIEKLDYLE
                     WLGVDCLWLLPFYPSPLRDDGYDVADFTTIAPSYGSTEVFRRLLDAAHQRGMRVIADL
                     VLNHTSDQHAWFQEARRSPQAPTRDYYVWSETDQKYTKARIIFVDTEKSNWTWDPEAQ
                     AYYWHRFFSHQPDLNYDNPAVKQAMLEVMEFWLDQGLDGFRCDAVPYLFEREGTLCEN
                     LPETHAYLKEIRKRIDDRYSTRILLAEANQWPSDVRPYLGNGDEFHMAFHFPLMPRLF
                     MGLRSEDRRPILDMFKHTPPIPSNCQWCLFLRNHDELTLEMCTGEERDYMYYAYARDP
                     QARRNIGIARRLAPLLENDRRKIELLNSVVFTLPGTPIIYYGDEIGMGDNIHLGDRNG
                     VRTPMHWTADRNAGFSKADPAKLFLPLVTDPVYGYQSINVDTQKQTPHSLLHWMRRMI
                     AIRKRHKVFGRGTLEFLAPSNEKILAYLRTYEAETVLLVHNLAESAQAVELNLTRFKG
                     IVPIELFGDSRFPQIGDHPYVLSLGPYGSYWFSLPAVRAWDSTYNLEWSAI"
     gene            2103..3347
                     /locus_tag="VN039_12280"
     CDS             2103..3347
                     /locus_tag="VN039_12280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV46772.1"
                     /translation="MTPADKNKPAKDDSTMTTALDDYREVSPKGTVDFLYRLSDLVKG
                     KSVVHVSAVRYGGGAAELLRRLVPMLTALGVEARWEVIAGTQEFFAVVKRLTDALQGR
                     DEKITEDMYQTFREINERNAKALNLEADMIMIHDHQPAGMVEYRASGAWLWRCHLDLS
                     QPQRRAWTFLRRYALKYDAAIFSLSGFAQRLPIPKFLMYPSIDPLSPKNREMSRSEIN
                     QIVDRLGIPRTKPIILQVAKFERFKDPLGAIRAYRIVKKHHDCRLVLAGSGIMHDPEG
                     EAVLAEAREAVGRDPDIHLVQLPPEADLEINALQRTATVVLQKSIREGFGVTVSEAMW
                     KGKPVVGGNAEGIAAQIIDEVTGYVVHSAEGAGFRLRHLLNNPGLIARMGAAGREHVR
                     RNFFITRQLGDFLTLLKIMPLQ"
     gene            3499..>4277
                     /locus_tag="VN039_12285"
     CDS             3499..>4277
                     /locus_tag="VN039_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HWV46773.1"
                     /translation="MNEPAEHLSKQAVTADVVVGLLTLNNAETIEQVVKSIIEGLQRF
                     FGGASAMIVNYDGGSQDGTPEIVERLAAGQAAVRIVPGAAGHYASLGTDSGLSGRADD
                     VRSLCETAQSLQAKVCLIIEGNLRSLSPQWIELLGRPVYHDGMDFVVPLFRRHRYEGT
                     LVNNLLYPLMRSLYGKRLRYPSGGGYGLSGHLARELIKNPFWSQDAARISFDGWMTTV
                     ALAEGYQVCHAFLGVKDQDSKLGTADLAHVLAAAVGAIFHS"
BASE COUNT          876 a         1162 c         1334 g          905 t
ORIGIN      
        1 gccgtggtcg atccattcga ctatccgaac ctggaggatc tgcgggaaga gattatttcg
       61 attatcgacg atcatctgtc acgaatgtcc actattcccc gtgtggtgtt cggcgagccg
      121 ttccatttca agcgttccag gattctggaa gtgccgatcg gtctggaagc gaggtccctg
      181 gcggaattcc gacaggtggt ggccgaagtc gatgtcagcg cgatctattt ccacatgttc
      241 gaagcgcatt tccggttggg gcgcgaagaa agcgacttct cggcctggat tcgaacgggc
      301 ctcggcttgc atgaactggc tgatcgtatt cgctccatca acccgtactt gggaagcctc
      361 gagcgtctgc ggtcgagcct tatcacggtg tgcgacgaat tcctggcgag aggatgaggg
      421 catgggatgc tgaaccaaga tcccctctgg tacaaggacg ccgtgtttta cgagctgcac
      481 gtgcgggcct tctacgacag caatgacgac ggcatcggag atttcgccgg cttgatcgag
      541 aagctcgatt atctcgagtg gctgggagtg gactgtctct ggctgctgcc gttttatccg
      601 tcgccattac gagatgatgg ctacgatgtg gcggacttca ccaccatcgc gccttcgtac
      661 ggatctaccg aggtgttccg gaggctgctc gatgcggccc atcagcgcgg catgcgcgtc
      721 atcgccgatt tggtgttgaa tcacacgtcc gatcagcatg cgtggtttca ggaggcccgc
      781 cggtcgccgc aggctccgac gcgggattat tatgtctgga gcgagacgga tcagaaatac
      841 acgaaggcgc gcattatctt cgtggatacg gaaaaatcca attggacctg ggatcccgag
      901 gcgcaggcgt actattggca ccggttcttc agccatcaac cggacctgaa ctatgacaat
      961 ccggcggtca agcaagcgat gctggaagtc atggagttct ggctggatca ggggctcgac
     1021 ggattccgct gcgatgcagt gccctacctg ttcgagcgcg aaggcacgct ctgcgagaac
     1081 ctgcccgaga cccatgcgta tctgaaggag atccggaaac ggatcgacga ccggtacagc
     1141 accagaattc tcttggcgga agccaatcag tggccgagcg acgtgcgtcc ttatttaggg
     1201 aacggtgatg agtttcacat ggcttttcac tttccgctga tgccgcgcct gttcatggga
     1261 ttgcgcagcg aggaccgccg gcctatcctc gatatgttca aacatacgcc gccgattccg
     1321 tcgaattgcc aatggtgcct gtttcttcgc aatcacgacg aattgacctt ggaaatgtgc
     1381 accggagaag aacgggatta catgtattat gcctatgccc gcgatcccca ggcgcgccgc
     1441 aacatcggca tcgcgcgccg tctcgcgccg ttgctcgaga acgaccggcg caagatcgag
     1501 ctgctcaaca gcgtcgtgtt taccttgccg ggcacgccga tcatctacta cggcgatgag
     1561 atcggcatgg gcgacaatat tcatttgggc gaccgcaacg gcgtgcggac cccgatgcat
     1621 tggaccgcgg accggaacgc cggcttttcc aaagcggatc ctgcgaagtt gtttcttccg
     1681 ctcgtcaccg atccggtcta cggctaccaa tcgatcaatg tggatacgca aaagcagacg
     1741 ccgcattcgt tgctccattg gatgcgccgc atgatcgcga tccgtaagcg gcacaaggtc
     1801 ttcggacgag gcacgttgga attcttggcg ccgtcgaacg agaagatcct ggcttatctg
     1861 cggacctacg aagcggagac ggtgcttctg gtccacaatt tggcggagtc ggcgcaggcc
     1921 gtggagctga acctgacgcg gttcaagggg atcgttccga tcgagctgtt cggagattcg
     1981 cgattcccgc aaatcgggga ccatccctat gtgctcagtc tcggtccgta cggttcctat
     2041 tggttcagcc tgccggcggt ccgagcctgg gacagcactt acaaccttga atggagcgcc
     2101 atttgacacc agcggacaag aataagccgg cgaaggatga cagcacgatg accaccgcgc
     2161 tcgatgacta ccgggaagtg tcgccgaagg gaacggtcga cttcctgtat cggctgagcg
     2221 acctggtcaa ggggaaaagc gtcgtgcatg tgagcgcggt ccgatatggc ggaggggcgg
     2281 cggagctctt gcgtcgctta gttccgatgc tgacggcgtt gggcgtggag gcgcgttggg
     2341 aagtcattgc ggggacacag gagttctttg cggtcgtcaa acggctcacc gacgccttgc
     2401 aaggacgaga cgagaagatc accgaggaca tgtatcagac cttccgggag atcaacgagc
     2461 ggaacgcgaa agcgctgaac ctcgaagcgg acatgatcat gatccacgac catcagccgg
     2521 ccggcatggt tgagtatcgc gcgagcgggg cttggctgtg gcgctgccac ctggatctgt
     2581 cccagccgca acggcgcgcg tggacgtttt tgcggcggta tgcattgaag tacgatgcgg
     2641 ctattttttc actatccggc tttgcccaac ggctgccgat cccgaagttt ctcatgtatc
     2701 cctcgatcga tccgctgagc ccgaagaatc gagagatgag cagatccgaa atcaatcaga
     2761 tcgtggatcg gctgggcatt ccgcgaacca aaccgatcat cctccaggtg gcgaagttcg
     2821 agcggttcaa ggatccgctc ggcgctatcc gggcttatcg cattgtgaag aagcaccatg
     2881 attgccgatt ggtgcttgca gggtccggga tcatgcacga cccggagggc gaagcggtgt
     2941 tggccgaagc gcgtgaggcg gtgggacggg atcctgatat tcatctggtg cagttgccgc
     3001 ccgaggcgga tctggagatc aacgcattgc aacgaacggc gacggtcgtg ctgcagaagt
     3061 ccattaggga aggattcggc gtgacggtct ccgaagcgat gtggaaaggg aagccggtgg
     3121 tcggaggaaa tgccgaagga atcgcggcgc aaatcatcga cgaggtgacg ggctatgtcg
     3181 tccattccgc cgaaggggcc ggattccgcc tgcggcactt attgaataat cccggattga
     3241 tcgcccgcat gggagcagcg gggcgggaac acgtgcgccg gaatttcttc atcaccaggc
     3301 aattgggcga ttttcttacg ctgttgaaga tcatgccgct gcagtgatta tggaggactg
     3361 agaattgagg actgggtgct gaggcattag gaccgagaca tcagttagat tttatcttgt
     3421 ttgtgtcata cgccatcagc ccggcacgcg aaccacgctt cacgaacgac gatatccaaa
     3481 atgaccgatc agtcagtcat gaatgaacca gccgagcatc tctccaagca ggccgtgacg
     3541 gccgatgtcg tcgtgggcct gctgaccttg aacaatgccg agacgatcga gcaggtggtg
     3601 aagagcatca tcgaagggct gcagcgattc ttcggcggcg cctcggcgat gatcgtgaat
     3661 tacgacggcg ggtctcaaga cggtactccg gagatcgtcg aacggcttgc cgcagggcag
     3721 gccgctgtcc gcatcgttcc cggtgcggcg ggacattatg caagccttgg gacggattcc
     3781 ggtctctcgg gacgggctga cgatgtccgc agtctgtgtg agacggcgca gtcgctccaa
     3841 gcaaaagtgt gcctcatcat cgagggcaat ctgcgctcgt tgtctcccca gtggatcgag
     3901 ctgctgggtc gtcctgttta tcacgacggc atggatttcg tcgttccgct gttccggcgg
     3961 caccggtatg aaggaacctt ggtgaacaat ctgctctatc cgttgatgcg ctcgctgtac
     4021 ggaaagcggc tacgataccc cagcggaggc ggctacggcc tttccggcca cctcgcgcgg
     4081 gagttgatca agaacccatt ctggtcacag gatgcggccc ggatcagctt cgacggatgg
     4141 atgacgaccg tcgcgcttgc cgagggctat caagtgtgcc atgcgtttct cggtgtcaag
     4201 gaccaggatt ccaaattagg aacagccgat ctggcccatg tgctggcggc tgccgtcggt
     4261 gcgatctttc attcgat
//
LOCUS       DATMTI010000772         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_453429, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000772 DATMTI010000000
VERSION     DATMTI010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453429"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..520
                     /gene="arfB"
                     /locus_tag="VN039_12290"
     CDS             110..520
                     /gene="arfB"
                     /locus_tag="VN039_12290"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWV46774.1"
                     /translation="MLLISPSVSIPDYEIDLHAVRSQGAGGQNVNKVSTAIHLRFDIR
                     TSSLPQIYKDVLLKLRDHRISSDGVITIKAQQYRTQERNREDALDRLRTLIQRAGTPR
                     KKRTGTAPTRNSQNRRIERKKRQGRLKVLRRTID"
     gene            complement(557..1213)
                     /locus_tag="VN039_12295"
     CDS             complement(557..1213)
                     /locus_tag="VN039_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46775.1"
                     /translation="MRMKTLILVLLAGGLSFPSMSLSAQQSGDRLAEQALEECNLGRL
                     TRDRATRLAHFQQGQILGERAVAAEEGSADAHFALFCNLGELLRINGESFTSLFGLYR
                     MMKELDRALEINPSHIGALSAKGTLLVRLPGLLGGDSEKGERLLQQVIKQAPKAVNAR
                     LALAKVRCEHGHHQEAVTLAADALAIAREYQQIEFIPEAEAMLQQARANAVKTKEPRS
                     "
     gene            complement(1285..2076)
                     /locus_tag="VN039_12300"
     CDS             complement(1285..2076)
                     /locus_tag="VN039_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HWV46776.1"
                     /translation="MLLKGKKGLVIGVANKHSIAWAIAQSTAGQGAQLLFNYQNERLK
                     QNVEDLAATLPGAKAFPCDVSNDGEITSLMQQVQKEVGQIDFLVHSLAFAPREELSGQ
                     FVNTTRQGFATALDISAYSLVAVARAALPLMPAGGSIVTLTYLGSERVVPHYNVMGVA
                     KAALEASVRYLAHDLGPLNIRVNAISAGPIKTLAARGVSGITKMVDLHKEFAPLRRPT
                     EQGEVGDAALFLVSQLGRGITGEVIYVDGGFNILGMMASGEQGIS"
     gene            complement(2260..>2474)
                     /locus_tag="VN039_12305"
     CDS             complement(2260..>2474)
                     /locus_tag="VN039_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribose pyrophosphatase"
                     /protein_id="HWV46777.1"
                     /translation="TAPGFADEVIHVYKATGLTKGRQQLDRDEVLEIVEMPLHLAITR
                     IQDGTIRDGKTIVGLQAVYIGDATNR"
BASE COUNT          545 a          692 c          672 g          565 t
ORIGIN      
        1 aggatcatgt ttctgtttgt tatgagacca ggctcaatgg gaaaattgaa cgaaatccgt
       61 tatgaggccg ctcattgcac cgacatatat gctatgctag ttattcagca tgctgctcat
      121 ttcgccgtcc gtttcgattc ccgattacga gattgatctt catgcggtgc gatcgcaggg
      181 ggccggtgga cagaacgtca acaaggtctc caccgccatc catctgcgat ttgatatccg
      241 tacgtcatct ttgccccaaa tctacaaaga cgttctgttg aagctgcgcg atcatcgcat
      301 atcatccgac ggggttatca cgattaaggc ccagcaatac cgtacacagg agcgcaaccg
      361 tgaggacgcg ttggatcggt tacggacgct cattcaacgg gcggggaccc cgcggaagaa
      421 acgaacaggg accgccccga cacggaattc tcagaaccga cggattgagc gaaagaagcg
      481 acagggtcgt ttgaaggtgc tgaggcgaac gatcgactga cgagggtggg acggtcttcg
      541 tctacgttga aacaggctag gagcgcggtt ccttggtttt tacggcgttg gctcgtgcct
      601 gctgcagcat ggcttctgcc tccggaatga actcaatctg ttggtattcc ctagcaatag
      661 ccaacgcatc cgcggccagc gtcacggctt cctgatggtg tccgtgctcg caccgaacct
      721 tggcgagtgc cagccgggca ttgacggctt tgggtgcctg cttgatgact tgttgcaaaa
      781 gtcgctcgcc tttttcagaa tcacctccaa ggagaccggg aagcctcacc agcaaggttc
      841 ccttggcaga gagcgcaccg atgtgagatg gattgatttc gagggctcgg tcgagttcct
      901 tcatcatgcg atagaggccg aaaagcgagg tgaaggattc tccattgatg cggagcagct
      961 cccccagatt gcaaaacagc gcaaaatggg catcggcgct tccctcctcc gccgccaccg
     1021 cacgttcgcc caagatctgt ccctgttgaa aatgagcgag gcgcgtcgca cgatccctgg
     1081 tcagtcgccc gagattacat tcttcgaggg cctgttcagc aagacggtcg cccgactgct
     1141 gtgccgaaag cgacatggat gggaaggaca ggccgcctgc caggagaact agtatgaggg
     1201 tcttcatccg catcactcag gcatccaacg ggaagacggc atcatatcgt ctggatttaa
     1261 cgctgatctt cgatcgccga caggtcagga gattccctgt tcaccggaag ccatcatgcc
     1321 caaaatattg aatcctccat ccacgtagat cacttcgccg gttatacccc gtcctaactg
     1381 actgactaaa aacaacgccg catccccaac ttcgccctgc tcggttgggc gacggagcgg
     1441 cgcaaactct ttatggaggt cgaccatctt ggtgatgccc gaaacccccc gcgcggccag
     1501 tgtcttgatc ggtccggcgg agatcgcatt cacccggata tttagagggc cgaggtcatg
     1561 tgccaagtag cggactgagg cctcaagcgc ggctttggcg acgcccatca cgttgtagtg
     1621 aggcacgacg cgctcgctgc cgagataagt gagggtgacg atagaacctc cggcaggcat
     1681 taacggcaag gcggctctcg cgacggccac gagagaatag gcactgatgt caagtgccgt
     1741 tgcaaatcct tgtcgagtgg tattcacgaa ttgtccgctc aactcctccc ggggagcgaa
     1801 tgccagggag tggacgagaa agtcgatttg accgacttcc ttctgaacct gctgcatgag
     1861 cgacgtaatc tcaccatcgt tgctcacatc gcaggggaat gctttcgcac cgggtaaggt
     1921 agcagcaagg tcctccacgt tttgctttag ccgttcgttc tggtaattga aaaggagctg
     1981 cgctccctga ccggccgtgg attgcgcgat ggcccaggcg atgctgtgct tattggccac
     2041 gccgatgacg agtccttttt taccctttag cagcatcaga acggttctcc ttcgttaaag
     2101 atcaagtcgg cattgatggg aaactccatc cctatttcac cgctagatgc cgcgccgtcc
     2161 ccaaatcgtt tggcagaata tcttccgtgc atacggttac tcaagcatta ccggaggatc
     2221 gcctaccttc tcttaatcgc tctcaatcag cagagtcgat tatcgattcg tagcatctcc
     2281 gatgtagacc gcctgtaagc cgacgattgt tttcccatct cggatcgtgc cgtcttgaat
     2341 tcgtgtgatt gccagatgca gcggcatttc gacgatctcc aatacttcgt ctcgatccag
     2401 ttgttgacgc cctttcgtga gaccggtggc cttgtagaca tgaatcactt catccgcaaa
     2461 acccggagcc gtaa
//
LOCUS       DATMTI010000773         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_453430, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000773 DATMTI010000000
VERSION     DATMTI010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453430"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..727
                     /gene="ruvA"
                     /locus_tag="VN039_12310"
     CDS             119..727
                     /gene="ruvA"
                     /locus_tag="VN039_12310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWV46778.1"
                     /translation="MIAFLTGRLAFKAPTHLTLDVHGVGYEVHIPLSTYYALPNLDEV
                     AALNIHTHLREDAIQLFGFLSQSEKESFLLLTSVSGIGPKLALNVLSSLSVTDLVHAI
                     QSEDVEKLATVPGIGKKSAGRIALELKDTVGKIHGIRPQATAAAAPELDGPYEDALSA
                     LVNLGYRAQDAKEALKRVIKAAPGSLGLKELIRNGLKELARG"
     gene            732..1220
                     /locus_tag="VN039_12315"
     CDS             732..1220
                     /locus_tag="VN039_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46779.1"
                     /translation="MPLNVRNIGQMLVYSISIGAVFVVAFPTRSLAQEATEPKSIRMT
                     CGTCPDGYATTGVTQSPEICKDDDPTLVQCVPLGANMLGVCGTCPDGYVEIGSSSVPA
                     RCGSKDGGRMTQCQLRKMEHNFPDSTQGYKRCPPDCGSTAQPGQGTLPPPPKYQQMQE
                     SK"
     gene            1237..>1886
                     /gene="ruvB"
                     /locus_tag="VN039_12320"
     CDS             1237..>1886
                     /gene="ruvB"
                     /locus_tag="VN039_12320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWV46780.1"
                     /translation="MTERFVTNRATDEERGQENVLRPQTLAEYVGQDKMKESLRICIE
                     AAKRREEALDHAIFYGPPGLGKTTIAHIIAREMGAALRSTSGLVLAHAGDLAAILTNL
                     QEHDVLFIDEIHRLPASVEEALYPAMEDFQLDLVIGQGPAARTVKLDLPPFTLVGATT
                     RAGSLTSPLRDRFGLVYRLEFYGPSELEAIVTRSAGVLGIGIDRAGAAEISRRARGT"
BASE COUNT          417 a          514 c          559 g          396 t
ORIGIN      
        1 cagaaggtcc acgcaaactt cgatattccg gatgaggtga tggagaaagt cgctgcgact
       61 acagcgggat aatcatcagc ttatcaccat ggttcttgcc ctcggataac gaaacgacat
      121 gatcgccttt ctcacggggc ggttggcatt caaggcaccc acccacctca cgctcgatgt
      181 gcatggggtc gggtacgaag ttcatatccc actcagcacc tattacgccc tgccgaatct
      241 cgacgaagtt gccgcgctga atattcatac gcatctccgt gaagatgcca ttcagctctt
      301 cggctttctc tcgcaaagcg agaaggaatc gtttttgctg ttgaccagcg tgtcgggcat
      361 cggcccgaag ctcgccctca atgtgctttc aagcctgtcg gtcaccgatc ttgttcatgc
      421 gatccagtct gaggatgtcg aaaagcttgc caccgttccc gggatcggga agaaatcggc
      481 gggtcgtatt gcacttgaac tgaaagacac ggtgggcaaa atccatggta ttcgtcccca
      541 agccactgcc gctgccgcgc ctgagctgga tggtccttac gaggatgcgc tttccgctct
      601 ggtaaacttg ggctatcgtg ctcaggatgc caaggaagcc ttaaagcggg tgataaaggc
      661 tgcgcccgga tcgttggggc tgaaagagct catccgcaac gggctcaagg aacttgccag
      721 ggggtaagcc tatgccgctc aacgtcagga acatcggcca gatgctcgtg tacagtatct
      781 ctatcggggc ggttttcgta gtcgcgttcc caactcggag tctcgcgcaa gaggccacgg
      841 agccgaaaag catcagaatg acctgcggaa cgtgccctga tgggtatgcg acgaccggcg
      901 ttactcagtc tccggaaatc tgcaaagacg acgacccgac tctcgtgcaa tgtgtgccgc
      961 tgggggcaaa catgctgggc gtgtgcggga cgtgtcccga cggctatgtc gaaattggaa
     1021 gttcctcagt gcctgctcga tgcgggagta aagacggtgg ccggatgacg caatgtcaat
     1081 tgaggaaaat ggagcacaat tttcctgact cgacccaggg atataaaaga tgtcctcctg
     1141 attgcggcag tacggcccag cctggccaag ggactctacc gcctcccccg aagtatcaac
     1201 agatgcagga gagcaagtag aggtgggcat cgcaccatga ctgaacggtt cgtgaccaat
     1261 cgcgccacgg acgaagagcg gggtcaggag aatgtgcttc gacctcagac actcgctgag
     1321 tacgtcggtc aagacaaaat gaaggaatcg cttcgaatct gtatcgaagc ggccaagcgg
     1381 cgggaagagg cactcgacca tgccatcttc tatggaccgc ccggtctcgg aaagacgacc
     1441 atcgcccaca ttatcgcgag ggagatgggc gcggcattga ggtctacatc gggtctggtc
     1501 ttagctcatg ccggcgactt ggcggcgatt ctgaccaatc ttcaggaaca cgatgtactc
     1561 tttatcgatg agattcaccg cctgcccgcg tccgttgagg aagcactgta tccggccatg
     1621 gaggattttc aactggacct ggtgattgga caggggcctg ccgcgagaac cgttaagctg
     1681 gatctcccgc cattcacact ggtaggggcg acgaccaggg ccggctctct aacatcccct
     1741 ctgcgagacc gattcggctt agtctatcgg ttggagttct atgggccgtc tgaactggag
     1801 gcgattgtga cgcgctcggc gggcgtctta ggtattggga ttgaccgggc aggcgccgcg
     1861 gaaatttcgc gccgagcgcg tggaac
//
LOCUS       DATMTI010000774         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_496532, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000774 DATMTI010000000
VERSION     DATMTI010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496532"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1991
                     /locus_tag="VN039_12325"
     CDS             <1..>1991
                     /locus_tag="VN039_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWV46781.1"
                     /translation="TPTLTNEQFQELQTRIQNTNIDIKLEILKHLSADQWDSTINRML
                     STDNPSTADLDQLHLVTSYKGQDVLRKTDRERPVLAEAIRKNKPILYNWFLKIAGDDT
                     RRKKLLNQTTPWGQTVLQTAILNDRFQTAKELMAAGATPSLDDAHLAASHGKRAFLER
                     TLLPALDMGKPRGFILEDPELEGPAENLEKQPRHEALMTLAARQQTDIQNHQQALQHR
                     RNESLLNWAKVGNHPAEHLVGLIRAGAQVNTLDSTHKTPLHYAVATGRGWLVEPLLLH
                     GANINIKTKNGNTVVHYAIAQRDIELLRLLLRYQPDLKLKHGAYPENSYLQTALETGD
                     NEIAKMIISHQPELIIQPNRHDQYPLKEAIRAGNEEIVEAMVAHYPDILYAQERDGSR
                     PQHWAASTGNVNMLALLFPKDIPVSQLRDGLGNSLLHLAAVRGQTDMVNFLIPQEKEN
                     LARPNHYHRTPLFEAVSSGNLDTVKAILKHHPTDTLQFSCDGYVPLHIALRKQEHRGI
                     VKALLAHDPESIQVRNKRKQNLLHLLPEYPHHAKLLETLTRQCPEMLEEADSDGMTPL
                     HYAVENQNLHAIRTFLATSPKLANQPTSRGELPIHRALVNYKEGLITELLKGGDKQSI
                     NVPNPTGEPPLYYAVKHYPRPTAMLKELLESGADINLADT"
BASE COUNT          564 a          569 c          497 g          361 t
ORIGIN      
        1 acaccaacgc tgaccaatga gcaattccag gaattgcaga ccagaattca aaatacgaat
       61 atcgatatta agctggaaat cctgaagcac ctcagtgccg atcaatggga cagcacgatc
      121 aaccgaatgc taagcaccga caacccgagc acggcggatt tagaccagtt acacctggtt
      181 acctcctaca agggtcagga tgttttaagg aaaaccgaca gggagcgacc ggttttagca
      241 gaagccatcc gcaagaacaa accgattctc tacaactggt tcctgaaaat tgccggtgac
      301 gatactcggc ggaagaagct cctcaaccag accacgcctt ggggacaaac ggttctgcaa
      361 accgccattt tgaacgatcg cttccagacg gccaaggagt tgatggcagc aggcgccacc
      421 ccttcgctgg atgacgccca tctggctgcg tcccatggca aacgggcctt tctggagcgt
      481 acattattgc ccgcgttgga tatggggaaa cctagagggt tcattcttga agatccagaa
      541 cttgaaggac ccgcagaaaa tctcgaaaaa caaccaagac acgaggcgct catgactctg
      601 gccgcgaggc agcagacgga tatccagaat catcagcagg cgctccaaca ccgcaggaat
      661 gagtccctgc tgaactgggc caaggtcggg aaccatcctg ctgaacactt ggtgggcctc
      721 attagggccg gtgcccaggt taatacgctc gacagcactc acaaaacccc actacattat
      781 gcagtcgcca ccggacgggg ctggctggtc gagcccctgt tgctacatgg ggccaatatc
      841 aatataaaaa ccaaaaacgg caacacggta gtacactatg cgattgcaca acgggatatc
      901 gaactgctca gattactgct gcgctaccag cccgacttga aactaaaaca cggggcatac
      961 cctgaaaatt catacttaca gacggctttg gagacaggcg ataacgagat cgccaaaatg
     1021 attatttccc atcaaccgga attgattatc cagccaaacc gacacgacca atacccgttg
     1081 aaagaagcga tacgcgccgg caacgaggaa atcgtggaag ccatggtggc gcactacccc
     1141 gacatcctat acgcccagga gcgagacggg agcaggccac aacattgggc ggcttcaacc
     1201 gggaacgtca acatgctggc cctattgttc cccaaagaca ttccagtttc ccagctccgg
     1261 gacggcctgg gcaattccct actgcaccta gctgccgtgc gaggccaaac ggatatggtc
     1321 aatttcctga ttccgcaaga gaaggaaaac ctggcccggc caaaccatta tcatcgaacg
     1381 cctttgttcg aggcggtttc ctccggcaac ctggataccg tcaaagccat tctgaagcat
     1441 cacccaaccg ataccctgca atttagctgc gatggatacg tgccactgca tatcgccctg
     1501 cggaaacagg aacatcgggg tatcgtgaag gccttgctgg cacatgaccc ggaatcgatc
     1561 caggtgcgaa acaagcgaaa gcagaacctg ctacatctgc tgccggaata tcctcaccat
     1621 gcaaaactgc tggaaaccct gacccgccaa tgcccggaaa tgctggagga agcagatagc
     1681 gacgggatga cgccactgca ttatgctgtg gaaaaccaga atttacacgc gatacggaca
     1741 tttttagcca ccagccccaa actcgcaaac cagccgacta gcagaggtga attgcccatt
     1801 catcgggccc tggtgaacta taaagagggc ttgattacgg aactgctcaa aggaggggat
     1861 aagcagagca tcaatgtgcc taacccgacc ggggagccgc cgctctacta tgccgtgaaa
     1921 cattacccac gcccaacggc gatgctcaag gaattgctcg agtcaggcgc ggacatcaat
     1981 ctggcagaca c
//
LOCUS       DATMTI010000775         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_707106, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000775 DATMTI010000000
VERSION     DATMTI010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707106"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VN039_12330"
     CDS             complement(<1..210)
                     /locus_tag="VN039_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46782.1"
                     /translation="MSEERSSSRRSKPIPQYPGPTRSEVFALSHEQVLNALERLKVEP
                     HKLPYEDADDDLFVLAYGQKALDDES"
     gene            complement(203..457)
                     /locus_tag="VN039_12335"
     CDS             complement(203..457)
                     /locus_tag="VN039_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46783.1"
                     /translation="MSANFKLYIPTPNHNKPLTDREIERECDKERQYHSLFFRGSVAL
                     CNCGKSYIDPTRRDIRWRHDHHVKLALEAFENKLRGISNE"
     gene            complement(787..1242)
                     /locus_tag="VN039_12340"
     CDS             complement(787..1242)
                     /locus_tag="VN039_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46784.1"
                     /translation="MTETTLTERLARRGKWRLARAKNRHHKQKIKHQDTAIDLTFPVR
                     LKPLAEKFYHQALNHPAFVYMTTSAIRQSIENRLWSIASQYPQWFGFGPLAKREATPA
                     QLAALTAARATRTSEAQDRERRLNERIIDEARGRARAEADEAATPSNEG"
     gene            complement(1374..1991)
                     /locus_tag="VN039_12345"
     CDS             complement(1374..1991)
                     /locus_tag="VN039_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46785.1"
                     /translation="MCTMLAMAGLSAAQAGASIYGQTQAQKQFNATESMRQQQQEVLI
                     EENRRRATYDFLNSVRLEQTQQSQEEAAVAQKSMDVERQATASIATGEASAAERGVAG
                     RSVDQIAQDFDFMANEESGRLQLNQKISNQQHAENIRSFGNDWSNRVASVKPYIPTPA
                     KPIDYFGPVFQAGAQVLSTDMAYSRANPTAPTMTNWLSAPTKKKA"
     gene            complement(1994..2245)
                     /locus_tag="VN039_12350"
     CDS             complement(1994..2245)
                     /locus_tag="VN039_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46786.1"
                     /translation="MIYRKRFSGTAARTTFSLTQETLDGLKKIRTAFGMRYPKDRYPT
                     LSLVLEQVLTKNLRELQENPEFLADEIEEFERRYAKRRK"
BASE COUNT          445 a          589 c          675 g          656 t
ORIGIN      
        1 agattcatcg tccaaagctt tctggccgta agccaaaacg aatagatcgt catccgcatc
       61 ctcataaggc agcttgtgcg gttctacctt cagacgctca agcgcattca acacttgctc
      121 atgactgagc gcgaagactt cagatcgtgt tggtcctggg tactgtggaa ttggtttgct
      181 tctgcgtgat gatgatcgtt cttcactcat ttgaaatccc tctcaactta ttctcaaagg
      241 cttccaatgc cagcttcacg tggtggtcat gtcgccagcg aatatccctg cgtgtcggat
      301 cgatgtagga cttcccgcaa ttgcataacg caaccgatcc acggaagaac aggctgtggt
      361 actggcgctc cttgtcgcat tcccgttcga tctcccgatc agtgagtggc ttgttgtgat
      421 tcggagttgg aatgtatagt ttgaagttcg cagacatggc ttaggcgctc ccttgcgacc
      481 ttggagcaag gtctccttct gagtggctac cttcaccatg tatctgtgct ttgaaagggt
      541 tcatcttctt tgttcctcct cttttgcttc tgtggttgct ctcgtcgttg gagtcgtcgt
      601 cgttcgtcag aacgaactcg ttcgtcaggc gtcaacgctc gtgtgacgtt cacctgatat
      661 gggagcttgc ctttgattcg tttgaccaga atggttccgt ttcggctgca tccgatcgcg
      721 gctctaagtt ccttctgacg ggctgcatac tcgcgctgat aggtcggatg cctatcgaac
      781 caagccttaa ccctcgttcg atggtgttgc tgcttcgtca gcttccgctc ttgctctgcc
      841 tcttgcttcg tcaatgattc tctcgttgag ccgtcgttca cggtcttgtg cctccgaggt
      901 tcgggtagcc cgtgccgccg ttagtgcggc gagttgtgcg ggggttgctt cccgtttagc
      961 gagtggccca aacccgaacc actgcggata ctgcgatgca atcgaccaca accgattctc
     1021 gatggattgg cggatcgcac tggtcgtcat gtagacgaag gccggatggt ttaaggcttg
     1081 gtgatagaac ttctcggcaa gaggcttaag acgaacgggg aaggtcagat cgatagcggt
     1141 atcctgatgc ttgatcttct gcttatggtg gcgattcttc gccctggcta ggcgccattt
     1201 tcctctacgg gctaggcgct ctgtgagagt tgtctctgtc atagtggtgt cctaagctag
     1261 gtggtctata agttattgga agtactgatg aatctggtat gagactttgg cggttatcag
     1321 cgcaacgggt tcggacagag aggaggcctc taggagccgt ctagaatggc ggcttaggct
     1381 ttcttcttcg taggtgccga caaccagttg gtcatcgtag gagcggttgg attggctcgg
     1441 ctgtaggcca tgtctgtcga tagaacctga gcaccagcct ggaacacagg cccaaagtaa
     1501 tcgatgggct tggccggtgt tgggatgtac ggcttaacag aagccacacg gttagaccaa
     1561 tcgttcccga aggagcggat gttctcagcg tgttgctggt tggaaatctt ctggttgagt
     1621 tgtaaccgtc cgctttcctc gttcgccatg aagtcgaagt cttgggcaat ctgatccact
     1681 gaccggccag caactccgcg ttctgcggcg gatgcttcac cggttgcaat cgaggctgta
     1741 gcttgtcgct ccacgtccat tgacttctgc gcaacggcgg cttcctcttg ggattgctgc
     1801 gtctgttcca gcctgaccga gttcaggaag tcgtaggttg cgcgtcggcg gttctcttcg
     1861 atcagaacct cttgttgctg ttggcgcatg gactcggttg cgttgaactg cttttgggct
     1921 tgagtttgtc cgtagatgga ggcaccggct tgcgcggctg acagaccggc cattgctaac
     1981 atcgtgcaca tgatcatttc ctccttttgg cgtagcgtcg ttcgaactcc tcgatttcat
     2041 ctgctaggaa ctcagggttc tcctgcagct ctcggaggtt cttcgttaag acctgttcaa
     2101 ggacgagtga gagagtcggg taccggtcct tcggatagcg cataccgaag gcagtgcgga
     2161 ttttcttgag gccgtcaagc gtttcctgag taagggagaa ggttgtacgt gcagccgtac
     2221 cactaaaacg tttgcggtag atcataagga acctctttgg gtaactgaac catccggcac
     2281 ttcggccaag atgggataag gtgaacggag tgaacggatg ggactgatga aggtgaaacg
     2341 gccttggacg aaggtgtgaa cttcg
//
LOCUS       DATMTI010000776         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_369272, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000776 DATMTI010000000
VERSION     DATMTI010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369272"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VN039_12355"
     CDS             complement(<1..686)
                     /locus_tag="VN039_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46787.1"
                     /translation="MPDIWNIGDPNDPVEKLKKALDVVGGVATPPAPDPPAWYTQEGQ
                     LGQAMQLGAKIVPGLGIVPDVVSTYLPHAPGVPKPPEAMTDDELKQAIATNPADTGAV
                     AEEKLRAASAGRNFLQNLTDPQTMSWAAGRLLKQVGAGTRNAVDIPAQLAGLYSLGSM
                     GAEKLADMVTPVEGKTHDFGSSAASDFANERYAEALRAADVSPPREFGESAANIAGGL
                     VPVGGSDIKGA"
     gene            complement(687..1700)
                     /locus_tag="VN039_12360"
     CDS             complement(687..1700)
                     /locus_tag="VN039_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46788.1"
                     /translation="MEAIGGIIASLLGVAAQAQAENDQKNLGYANLFETKRENREREK
                     LAESSQTDAYGNKIYYDPLTRAWKIQSTPTTKNILDAEQSEQLKSLTSDAARNRSAAI
                     RKDQRAQQANDLFTQALTDYKYRPQQSEADYVGDATKEALLSRRAGAAEAAAAVNKAL
                     IRAGNGSQIPAVFNAARDADANEFEQTLLGAKRQGEQDFLNIGGAKDQKAQQQIDFLR
                     SIADDTSDSPIHFGNENDTLANRSSGALSQLLSVLAQDSANRQGAVGPLMDALGRSPD
                     FSGLGSALGRLGSMGGQQTTSAYPSKTTPQGTIDGGWWQTMGQMGYLTPSSSSASAFM
                     NEF"
     gene            complement(1756..>2129)
                     /locus_tag="VN039_12365"
     CDS             complement(1756..>2129)
                     /locus_tag="VN039_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46789.1"
                     /translation="PKSLSKSKRARTDPPGRGPSKSGFADLQRGRMDPMKELQQIIAV
                     AGADPVMAGPRNVLPQMLQSQQMRGMAAQNGLPGAGGAPAIDPTTAAILQKILGAGAG
                     MPPGISDGQDQEPDNDPDDGE"
BASE COUNT          461 a          607 c          549 g          512 t
ORIGIN      
        1 gcacctttaa tgtcactacc accaacggga acaagcccgc cagcaatatt agctgcggac
       61 tcaccaaatt ccctaggcgg ggacacatct gcggcacgaa gggcttcggc gtaacgctca
      121 ttagcaaaat ccgaagctgc cgatgagcca aaatcatgcg ttttgccctc aacgggcgta
      181 accatgtccg cgagcttctc agcacccata ctgccaagac tataaagccc cgcaagctgt
      241 gcagggatat ctacggcatt gcgtgttcca gcaccaactt gcttaagaag cctacctgcc
      301 gcccacgaca tagtctgtgg atcagttagg ttctgcaaga agttgcgtcc agcactcgcg
      361 gcgcgaagct tttcttctgc tacagcccct gtatcagccg ggttcgtagc aattgcctgc
      421 ttaagctcgt catcagtcat cgcctcagga ggttttggca ctcccggagc atgaggtaga
      481 tacgtagata caacatcagg cacgatgcca agaccgggta cgatcttagc accgagttgc
      541 atagcttgac caagctgacc ctcttgcgta taccacgcag gtgggtcagg agccggtgga
      601 gttgctacgc ccccaacaac atcaagggct ttcttaagct tctccaccgg atcgttggga
      661 tcgccgatat tccaaatatc tggcatttag aactcgttca tgaacgctga tgctgaacta
      721 gacgacggcg taagatagcc catctggccc atcgtctgcc accaaccacc atcgattgtg
      781 ccctgaggcg tagttttcga tggataagca cttgttgtct gttggccacc catgctccca
      841 agcctaccca atgctgatcc tagaccacta aagtccggtg aacgcccaag agcgtccatc
      901 agtgggccta cagcaccctg acgattggct gagtcctgag ccaagacact aagcaactga
      961 gacaatgcac cacttgacct atttgccagc gtatcattct cgttaccgaa gtgaattggt
     1021 gagtcactcg tatcatcggc gatactacgc aagaaatcaa tctgctgttg agctttttga
     1081 tccttggccc caccgatatt gaggaaatcc tgctctccct gacgcttggc tccgagcaac
     1141 gtctgctcaa actcattcgc atcagcgtca cgagcggcat tgaaaaccgc cggtatctgc
     1201 gagccattac cagcacgaat tagtgccttg ttgaccgcag cggccgcttc cgcagcaccc
     1261 gctctgcgag aaagtagagc ctccttcgta gcgtcaccaa cgtaatcggc ctcagattgc
     1321 tgagggcggt atttgtagtc ggtaagagct tgagtgaaaa gatcattggc ctgttgagcg
     1381 cgttgatcct tacgtattgc agcagagcgg tttcgagcag catctgatgt gagggattta
     1441 agttgttcag actgctcggc atcgaggata ttcttggtgg ttggtgtaga ctggattttc
     1501 cacgcacggg tgagtgggtc atagtaaatc ttgttcccat acgcgtcggt ttgcgagctt
     1561 tcggcaagct tttcacgctc acggttctca cgcttggtct cgaacaggtt cgcgtagcca
     1621 aggttcttct ggtcgttctc agcctgtgcc tgagccgcaa caccgagtag tgatgcgata
     1681 attccgccga tggcttccat gctacaggtc ctgtattaga ggtttagccc ggcgaataaa
     1741 tcgtcgggcc ttttattatt cgccatcatc gggatcgttg tcaggttcct gatcctgacc
     1801 atcactgatg cccggaggca tcccggctcc ggctccgagg attttctgta gaatagctgc
     1861 cgtcgtagga tcgatcgccg gtgcaccacc tgcacccgga agcccattct gtgcagccat
     1921 tccgcgcatc tgctggctct ggagcatctg cggcagcaca ttcctcggtc cagccattac
     1981 aggatcggca ccagcgacgg cgatgatctg ctggagttcc ttcatcgggt ccattcgtcc
     2041 ccgttggagg tctgcaaatc ccgacttgga aggcccccga ccgggagggt ccgttcgggc
     2101 acgcttggac ttgcttagag actttggtg
//
LOCUS       DATMTI010000777         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_750466, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000777 DATMTI010000000
VERSION     DATMTI010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750466"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1624
                     /locus_tag="VN039_12370"
     CDS             23..1624
                     /locus_tag="VN039_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HWV46790.1"
                     /translation="MSAIDSRHPRYSEWMLTWQKLRDFYEGEERVKDQGELYLHPTEG
                     MILDGMKGQGDAKQLGLKRYEAYKARAVVPEYVETAVELLLGLLHQKPATIKLPPEME
                     PLRTVCSANRESLDQFQRRINVEQLITGRFGIMCDIKKDATPTDLPYMALYSTERIIN
                     WDDNDEMAGPNALNLVVLDESKLTRVDFEWKFTNAKRVLQLGPILTNEPNDAGFVYKQ
                     GYFSNADSTSVQYVEADMVAPMYRGRTLQEVPFVIGNTKDLLSKPDKPPLNSLCNTCI
                     AIYQQEADYRQNLHMQSQDTLVVIGGTRNSSTDPGQPDALRVGSGSRIDVDQGGDAKF
                     IGVSSLGLPEQRQAIENDRKRAIIKSGELIQSGTGSQQESGSALRTRFTAQTATLNQI
                     ALTGAEAIQYALRLIARWIGADETQVEVIPNLEFVDFGLDGLDFLNLMQAKTLGFPVS
                     YESLHRTAQDRGLTTMDFKSEIAQIKTENTTIADVIKPPPPAPPPGAPAPSVPPGIDP
                     TKKTNVPPNPRENPGVPNSKPKGGQ"
     gene            1691..2503
                     /locus_tag="VN039_12375"
     CDS             1691..2503
                     /locus_tag="VN039_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46791.1"
                     /translation="MALKAVLDNLEGLPADVASEYVEKNGKFELQVEGMKTQGDIDRI
                     QGGLTKERSDHKATKDRLSAWGELDPTEVLGKLSRFDELEAAAKGKLDDAGIQKIVDG
                     RLNSVKAPLERQVQQLTAKLGEATSAIEGFQTQNRVRTIGDAVRSAAVKTKVLESAVE
                     DVMMYGERLFEITEDGQILTRDGVGVTPGVSAEVWLSDMATKKPHWFPGSSGGGAGGS
                     TGAAGGGNNPFSREHWNMTEQGKMLTSNRAKAEQMARAAGTTIGGPMPAPRK"
BASE COUNT          628 a          744 c          763 g          492 t
ORIGIN      
        1 cggcacctaa cggagaacag atatgtctgc aattgacagc cgccatcctc gatacagcga
       61 gtggatgctc acttggcaga agcttcgtga cttctacgag ggcgaggagc gcgtcaagga
      121 ccaaggcgag ctttacttgc atccgacgga aggcatgatc cttgacggca tgaaaggcca
      181 aggagacgcc aagcagttgg gcctcaagcg ttatgaggcg tacaaggcgc gcgccgttgt
      241 gcccgaatat gtcgagactg cggtcgaact tttgctcggc ttgcttcatc agaagcctgc
      301 aacgattaag ttgccaccgg agatggaacc tcttcgtact gtctgttccg ccaaccgcga
      361 atctctcgac cagttccaac gtcgtatcaa tgtggagcag ctgatcaccg gtcgtttcgg
      421 tatcatgtgc gatatcaaga aggacgcgac tccgactgat cttccgtaca tggcgctcta
      481 ttccacggag cgaatcatca actgggacga caacgacgag atggcgggtc caaacgccct
      541 caaccttgtg gtgttggacg agagtaaact gacgcgcgtg gatttcgaat ggaagttcac
      601 gaatgcaaag cgcgttctcc agctcggtcc gatcctcaca aacgaaccca acgacgcagg
      661 ctttgtctac aagcaaggat atttctccaa tgcagattcg acctccgtgc aatacgtaga
      721 ggccgatatg gttgctccga tgtaccgggg caggacgctg caagaagttc cttttgttat
      781 cggcaacacg aaagaccttc tttccaagcc cgacaagccc cctctcaact cgctgtgcaa
      841 tacctgcatc gcaatctatc agcaggaggc ggattaccgt cagaatctcc acatgcagtc
      901 gcaagacacg cttgtcgtga ttggcggtac acgcaattcc tcgaccgatc cagggcagcc
      961 cgacgcgctg cgcgtaggat caggctcgcg tatcgatgtg gatcaaggcg gagacgccaa
     1021 gttcatcggt gtgagctcgc taggcttgcc tgaacagcgt caagcgattg agaacgatcg
     1081 caagcgtgca attatcaaga gcggcgagct gattcagagc ggtacagggt cgcagcagga
     1141 aagcggtagc gcactacgca cgcgcttcac tgcacagacc gcaaccctga atcaaatcgc
     1201 actcactggc gcagaggcga tccagtacgc acttcgcctg attgctcgat ggattggtgc
     1261 agacgagacg caagtcgaag tcattccgaa cctcgagttc gtggacttcg gtctggatgg
     1321 cttggacttc ctcaatctga tgcaggccaa gacgctgggc ttcccggtct cgtacgaaag
     1381 tctccatcgg accgcgcaag accgcggcct gacgacgatg gacttcaaga gcgagattgc
     1441 tcagatcaag actgagaaca ccacgatcgc agacgtgatc aagcctccgc cccccgctcc
     1501 gcctccgggt gcgcccgctc cgagtgttcc tccaggcatc gatccaacca agaaaacgaa
     1561 cgtccctccc aacccgaggg agaatcctgg cgtgccgaac agcaagccga aaggcggaca
     1621 gtaacaaggc cgcagatgac tgtggtcacc atcgcggcat gtgccgcaca acaaagaaga
     1681 ggaaagagtc atggcactca aagcagttct cgacaatctg gaaggtcttc ccgcagatgt
     1741 cgcttccgaa tacgtggaga agaacggcaa gttcgaactt caggtcgaag gcatgaagac
     1801 ccagggcgac atcgatcgta tccagggcgg tctcaccaag gagcgttctg accacaaggc
     1861 taccaaggat cgcttgtccg cttggggcga gctggatccg acggaagtgc tgggcaagct
     1921 cagccgtttc gacgaactcg aggctgcggc caagggcaag ctggacgatg ccggcatcca
     1981 gaagatcgtc gacggtcgtc tgaacagcgt caaggcgcct ctcgagcgtc aggtgcagca
     2041 gctgaccgcg aagctgggcg aagcgacgtc cgccatcgag ggcttccaga cgcaaaaccg
     2101 cgttcggacg atcggtgatg cagtgcgttc cgcagctgtc aagacgaagg tgctggaaag
     2161 cgctgtcgaa gatgtcatga tgtacggcga acgactgttc gaaatcaccg aagacggtca
     2221 gatcctgacc cgcgacggtg taggcgtgac gcctggtgtc tctgcagaag tctggctgtc
     2281 cgacatggcg accaagaagc ctcattggtt ccccggttcg agcggcggcg gtgcgggcgg
     2341 tagcactggg gcggcaggtg gcgggaacaa tccgttcagt cgcgaacact ggaacatgac
     2401 cgaacaaggc aagatgctca cctccaatcg cgccaaggca gagcagatgg cgcgtgctgc
     2461 cggcaccacg attggcgggc cgatgcctgc cccgcgtaaa taatcaaaca tttgcgcttg
     2521 cacaacgata tggctcatgt tacattgcgt ggcgtgtggc atgagccata tcacatacaa
     2581 ccgggccagg tcatgggact gaggcttcga gtttctactt gaaccca
//
LOCUS       DATMTI010000778         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_242761, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000778 DATMTI010000000
VERSION     DATMTI010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242761"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..847
                     /gene="thiE"
                     /locus_tag="VN039_12380"
     CDS             173..847
                     /gene="thiE"
                     /locus_tag="VN039_12380"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWV46792.1"
                     /translation="MAQASGEKHPSASILAGLYVILDPSVCSDRPLVDVLKGSATAGA
                     KIFQYRNKTASMKAAYAEALPLRKAAHELGVLFIVNDRCDLALAVDADGVHLGQGDLP
                     LNLARKVMGPAKLIGISTHSREQVLAATAGGPNYLGFGPIFMPGSKLDHDPVVGLQGL
                     REIRPLTALPIFAIGGITADRTEEVIRAGADGVAVISAILKAPDISQSVTDFISRVST
                     PTSPGS"
     gene            complement(807..1535)
                     /gene="bioD"
                     /locus_tag="VN039_12385"
     CDS             complement(807..1535)
                     /gene="bioD"
                     /locus_tag="VN039_12385"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HWV46793.1"
                     /translation="MNHGVFITGTDTGVGKTLVTAALALHLKKRGLAVGVMKPIETGV
                     SAGREARSDATRLRSIIESEEAMGAICPYSFELPVAPLTAAQMEGFSINPGTISKIYR
                     LLSSRYECMVVEGVGGVQVPITHSDNVLSLIKHLRLPVVVVGRSELGGINHALLTVEA
                     LRRQKIRMIALVLNRTHPVRSALARVQERSTVEILRRQSGMPVIGPLPYESGLPARFR
                     PATTRLARSAAFKNLAKLVLRPAR"
     gene            complement(1646..>2202)
                     /locus_tag="VN039_12390"
     CDS             complement(1646..>2202)
                     /locus_tag="VN039_12390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV46794.1"
                     /translation="PYYADIREAESVRRMIDRFSSCASGPLVLICNAGIGQSDLLVRH
                     SDEIWENVIATNLTGTFHCLRAMAPSLLARGGGSIIVIGSHTGFHGATGQSAYATSKA
                     GLIGLVRTAAREWGPQNIRVNLVLPGWQKTDLTEGIFPEGSGWPDHALRRPPTVEEAA
                     STIVHLAQLKDISGQIWNCDSRHL"
BASE COUNT          459 a          606 c          652 g          485 t
ORIGIN      
        1 aactacaagc ggcgggggaa gggaaccaga caggtgctga tttcgaactg gaggctgaaa
       61 ttgcatccat ggtgccggat tggctcgtgg caatcgccat acggcaggaa ctggaaatgc
      121 actttcgtat cgtcagacag aaggcgttag agcattcaaa gcgataactg atatggctca
      181 ggcttcggga gagaagcatc cgagtgcatc gatactggcg ggtctctatg tcattctgga
      241 tccgtcggtt tgttccgatc gtccgcttgt cgatgtccta aaaggttccg caacggccgg
      301 cgccaaaata tttcagtacc gaaacaagac ggcttcgatg aaagcagcct atgcggaggc
      361 gttgccgctg aggaaagcgg cgcatgagct cggcgtcctg ttcatcgtca atgaccgatg
      421 cgatttggcg ctggcagtgg atgcggacgg tgtgcatctc ggacagggag acttgccgct
      481 caatctcgca cgaaaggtca tggggcctgc caagctgatc ggtatttcga cacacagccg
      541 agagcaggtg ttggcggcaa cagccggcgg gccaaactat ctcggatttg gtccgatttt
      601 catgcccggc tcaaagttgg accatgatcc ggtcgttggt ctgcaaggac tacgagaaat
      661 ccgccctttg acggcgctcc cgatcttcgc catcgggggg attacggctg atcgcaccga
      721 agaagtgatt cgagcaggag ccgacggcgt ggcggtcatc tccgccattc tcaaggcgcc
      781 ggacatttcg cagtctgtta ccgactttat ctcgcgggtc tcaacaccaa cttcgccagg
      841 ttcttgaacg cagcggatcg ggcaagacgg gtggtagcgg ggcggaatcg tgccggcagg
      901 cctgattcgt aagggagtgg gccgatcaca ggcatgccgg actgtctgcg aaggatctca
      961 accgtcgatc gttcttgaac gcgcgcgagg gccgatcgga cgggatgtgt gcgattcaga
     1021 acgagagcga tcatccggat cttctgtcgc cgaagggctt cgacggtcag caaggcatga
     1081 ttgattccgc ccagttcgga tcgcccaacg acgacaacag gaagtcgcag gtgtttgatc
     1141 aagctcagca cgttgtcgct atgtgtgatg ggtacctgca caccgccgac tccttcgacc
     1201 accatgcact catatcgact ggataggaga cgatagatct tgctgatggt gccgggattg
     1261 atgctgaatc cttccatctg tgcagcggta aggggagcga ccggcaactc aaacgaatag
     1321 ggacagatgg ctcccattgc ctcctcgctt tctatgatgg agcgtaaccg ggttgcatcc
     1381 gaccgagctt ctcttcctgc cgagactccc gtctcgatcg gtttcatcac gccgaccgcc
     1441 agtccgcgct tcttcagatg cagggcgagc gccgcagtga ccaatgtctt cccaacgcct
     1501 gtatcggtac cggttatgaa gacgccgtgg ttcatggaag agagccgatg gcagatagca
     1561 tgtggcgtat ggttcggagt gatcaactcc tgttatgaat ctcctctggc tatcagctat
     1621 aggccataag ctatacgctc ttttctcaca gatgcctgct gtcgcagttc caaatctgtc
     1681 ccgaaatatc tttgagctgg gccaggtgca cgatggtgct ggcggcttcc tcgaccgtcg
     1741 gcggtcggcg caatgcgtga tccggccatc cactgccttc cgggaatatt ccctcagtca
     1801 agtcggtctt ctgccagccg ggcagcacga gattcactcg aatattctgt ggcccccatt
     1861 ctcgcgcggc cgttctgaca agcccgatca gtccggcctt cgatgtcgca tacgcacttt
     1921 ggccggtcgc cccgtggaat cccgtgtgag acccgatcac aatgatggaa cctccgccgc
     1981 gagcgagcaa ggaaggcgcc atggctcgca ggcagtgaaa tgttcctgtg agattcgtgg
     2041 cgatgacgtt ctcccagatc tcgtctgaat ggcgcacgag caaatcgctc tgtccgattc
     2101 cggcgttgca aatcagtacc aatgggccgg atgcgcagct ggagaatctg tcgatcatgc
     2161 gccgaacgga ttccgcttct cgaatatctg catagtatgg gt
//
LOCUS       DATMTI010000779         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_326827, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000779 DATMTI010000000
VERSION     DATMTI010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326827"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /gene="dprA"
                     /locus_tag="VN039_12395"
     CDS             <1..858
                     /gene="dprA"
                     /locus_tag="VN039_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWV46795.1"
                     /translation="IGKINRQSIVTSNFTAILQRYEDMPAELYVAGKLPNKRITSVAI
                     VGSRKPTSYGEEVTYRFAYDLARQGVVIISGLAYGIDAIAHQAALDAGGITIAVMANG
                     LHRIYPFQHTSLAEKIVQQGGALISEQALGIEAHPHHFLARNRIVSGLADAVLVTEAT
                     DRSGTFSTVAHALTQNKEVFAVPGPITGLLSAGPNRLLQDGAQVALSAEDILSVITPH
                     HIPEQRILPLGDTPLEVCIIELLQKGIRDGDMLLAKSGASSSEFLSTLTMMELKGIIQ
                     ANGGQWHLR"
     gene            991..1392
                     /locus_tag="VN039_12400"
     CDS             991..1392
                     /locus_tag="VN039_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46796.1"
                     /translation="MTAIATLQKLEEFARNMQYYEFHGSIPETTVLNYRDGYREETMT
                     YRIGIIETECGRLVTFRKADSSFGGCGLIQATKPEVINVYEQHELLPLLSTTTWRAVA
                     QDFAVLKDSLDMAITGSARETTRLLEEAHMS"
     gene            1589..2035
                     /locus_tag="VN039_12405"
     CDS             1589..2035
                     /locus_tag="VN039_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46797.1"
                     /translation="MAIKSKEDLTEVERLEKALRAFRSKTPKIGLMQRGDTYPVFDIT
                     NPAKTRVTDENGRKLERRHLWQYPISPLGQLWQVIVCRGEHESFSVARYAILDHHGNH
                     QDMTSDQLARFVKDPPKKLEINLEVLVDVAEKAAVNVRLGRNPDHI"
BASE COUNT          520 a          609 c          537 g          418 t
ORIGIN      
        1 atagggaaaa tcaatagaca aagtatagtt actagcaact ttactgcaat cctccagagg
       61 tacgaggaca tgccggccga actatacgtg gcagggaaat tgccaaataa gcgaattact
      121 agcgtggcaa ttgtagggtc gcgaaaacca actagttacg gggaagaagt aacctatcga
      181 tttgcttacg acctagcgcg acagggtgta gtgattataa gtggcttggc gtacggcatc
      241 gatgccattg cccaccaagc cgcactcgat gcaggcggca ttacgattgc cgtcatggcc
      301 aacgggctcc accgcattta ccccttccaa catacttcgc tcgccgagaa aatcgtccaa
      361 cagggcgggg cactcataag cgaacaagct ttgggcatcg aggcacaccc gcaccatttt
      421 ctggcccgta accgtatcgt gagcggccta gctgatgccg ttttagtcac tgaggccacc
      481 gaccgtagcg gtactttttc aaccgtagcc cacgccctaa cgcaaaataa agaagtcttt
      541 gccgtccccg ggcccattac agggttgctg tcagcaggcc caaaccgatt acttcaagac
      601 ggggcacagg tggcattaag tgccgaggat attctttctg ttattacccc tcatcatatt
      661 ccagaacaga ggatactacc ccttggtgat accccgcttg aagtatgtat tattgaactc
      721 cttcaaaagg gtatccgcga tggtgacatg ctcctcgcaa aatcgggagc ctcatcgagc
      781 gaatttcttt cgacccttac gatgatggaa ttgaagggga taatacaggc aaacgggggt
      841 cagtggcatc tgcgataagt tgcatttaat cataaattat gttataaaat accaagcgtc
      901 gagcttcaat cacgcacctg tgttacccaa aataacccat ctccgttcga agctccgcga
      961 gaacttcgaa ccagacagaa gagagtcacc atgactgcta tcgccacgct ccagaagctc
     1021 gaggaattcg cccggaacat gcagtactac gagttccacg ggagcatccc tgagacgacg
     1081 gtcctgaact accgcgacgg ctaccgcgag gagaccatga cctaccgcat cggcatcatc
     1141 gagaccgagt gcggccggct cgtcacgttc cgcaaggcgg actcgtcctt cggcggctgc
     1201 gggctcatcc aggctaccaa gcccgaggtc atcaacgtct acgaacagca cgagctgttg
     1261 ccactgctca gtacgacgac ctggcgcgcc gtcgcccagg acttcgccgt gctcaaggac
     1321 agcctcgaca tggccatcac tggcagcgcc cgcgagacga cacgcctcct cgaggaggcg
     1381 cacatgtcct gagtccagcg gcgcgagctc tcactgttca cccctcgttc gaagtgccgg
     1441 ccctttcggg ggccggttct tcatattgcg actttttata aaaaatgata taatatattt
     1501 accgtgctcc tgcaacccgg tgagcacacc ccgcactttg gaactctcaa acccctggcg
     1561 caagcccgat gaagaaaggt ggtggcgtat ggccatcaaa agcaaggaag acctgaccga
     1621 ggtcgagcga ctggagaagg ccctcagagc cttcaggtcc aaaaccccca agatcggcct
     1681 gatgcaacga ggggacacct acccggtgtt cgacatcacc aatccagcca agactcgggt
     1741 aaccgatgag aacgggcgga agctcgaacg ccgtcatctg tggcagtacc cgatcagtcc
     1801 cctcggacag ttgtggcagg ttatcgtctg cagaggcgag cacgagtcct tcagtgtcgc
     1861 acgctacgcc atcctcgacc atcacggcaa ccaccaggac atgaccagcg atcaactcgc
     1921 tcggttcgtg aaggatccgc cgaagaaact cgagatcaac ctcgaagttc tcgtggacgt
     1981 ggctgaaaaa gccgcggtga acgtgcgtct cggtcgtaac cctgatcaca tctgaagttc
     2041 tgaagtgcca agccggcccc tttcgagggg gccggctact tcat
//
LOCUS       DATMTI010000780         1820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_326832, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000780 DATMTI010000000
VERSION     DATMTI010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326832"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VN039_12410"
     CDS             complement(<1..786)
                     /locus_tag="VN039_12410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV46798.1"
                     /translation="MPTTYQERFGKNDDLIHAYADDNSPRVSEIQYLYDAIKRLEARR
                     VLNVPFEGSLIKTVATNEQVTFADFVVPATLRKWNILKTDFDLTGIRKNYFDAVISIA
                     GIHHLTDQEQFQFIAATRKALRVGGRLLMVEVTTDSPTSRFLDGFVGKHTPTGHVGNY
                     LKNDFVRIVKLAGYHKIRRATVIHEWVFRDKEHLYAWMTKFFGVSVPKQDLIAQVDGI
                     LGINKRKGCLTVNWPLDFVGAQAERTPRKIPSPHRQDKIAQSRL"
     gene            1125..1673
                     /locus_tag="VN039_12415"
     CDS             1125..1673
                     /locus_tag="VN039_12415"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV46799.1"
                     /translation="MRLVVLVGVLTLTSASVMAAADSVRIDSKKTIPAIPLAGEEPYE
                     ALKRGDFRLAAGLFYPLAERGDARAQYNLGLLYASGLGVTHDYQAALKWHRMAAKQGH
                     AGAQNELAQMYSKGQGVQQDHIRAHMWYSIAAESLSGGSKQELEKDREKTVSRMTPAQ
                     IQKAQELARECLESKLKKCDEK"
BASE COUNT          446 a          442 c          513 g          419 t
ORIGIN      
        1 caggcgggat tgggcgattt tatcctgacg atggggagaa ggtattttcc gaggtgttct
       61 ttcggcctgt gcgccgacaa agtccaacgg ccaattcacc gtcaaacacc cttttcgctt
      121 gttgatcccc aagatgccgt cgacctgggc aatgagatcc tgtttgggga cagagacacc
      181 aaaaaatttg gtcatccagg cataaaggtg ttctttgtcc cgaaataccc attcatggat
      241 cacggtcgct ctccggatct tgtgatagcc ggccagcttc actatccgga caaagtcatt
      301 tttgaggtag tttccgacgt gtcccgtagg agtatgtttt cccacaaatc cgtcaaggaa
      361 acggcttgtg ggggaatcag tcgtcacctc taccattaac agccgtccac cgaccctaag
      421 tgcctttcgg gtggctgcaa tgaactgaaa ctgttcctga tccgtaagat gatgtatacc
      481 tgcaatggat atcaccgcgt cgaaatagtt ctttcgtatg ccggtcaagt cgaagtctgt
      541 tttgagaata ttccatttgc gtaacgtagc cggaacgacg aaatccgcaa acgtaacctg
      601 ctcatttgtc gcgacggtct ttatcaggct gccttcgaat ggaacattga ggactcgtcg
      661 cgcttcgaga cgttttatcg cgtcatagag atattgaatc tccgacacgc gaggagaatt
      721 atcatcggcg taagcatgga tgaggtcgtc gttcttcccg aatcgttctt ggtacgtcgt
      781 cggcatggct cacctcgatc gtcgtttcgc gggcagggtt tctccgatca cgtccttact
      841 aggctgatcg gtgtacttat cagagcaagc caattgctac aactcaaaag gtgtcatgcc
      901 aaagtagggg gcatggagca agagatgtgg gttccgggtt ccatagaata cgcaaacgga
      961 cgaggcggtt gcattggctt gcccaagcct gtgtcacagc gatcgcaatc aataacaata
     1021 ggacgaccga acggtgcgtt gtagaagtag atgaaatgcg gacaatggtt gtattgtaag
     1081 cgagcgtaag gcattctaag tcattgctga aaggcaggta cacgatgagg ctagtcgtgc
     1141 tcgtaggcgt gttgaccttg accagtgcgt ccgtcatggc tgcagcggac tcggtacgta
     1201 ttgattcgaa gaaaacgata ccagcgatac ccttggccgg agaggagcca tacgaggcgc
     1261 tgaagcgtgg agatttcagg ctcgcggcgg ggctctttta ccccttggcg gagagaggag
     1321 atgcacgagc gcagtacaat ctcgggctac tatacgccag cggtctggga gtgacgcacg
     1381 actaccaggc ggcgctgaaa tggcatcgca tggccgcaaa gcagggtcat gcgggagcgc
     1441 agaacgaatt ggcgcaaatg tattccaagg gacaaggcgt tcagcaggac catattcgtg
     1501 cccatatgtg gtacagcatc gcagcggaat cgttaagcgg cgggtccaaa caagagttgg
     1561 agaaggatcg agaaaagacg gtatcacgga tgactccggc tcagatccag aaagcgcagg
     1621 agttggccag agagtgccta gaatcgaagc tcaagaagtg tgacgagaag taacgcccgc
     1681 agcataagtt gaagccatgt cgccggcgac cacaccctgc cggaccagtt tttatctcag
     1741 cgcgccattg aactgtctcg ccatagatac attcctcttg cggagttcca tccagtaggt
     1801 tgacatggtg gttcaccaag
//
LOCUS       DATMTI010000781         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_242843, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000781 DATMTI010000000
VERSION     DATMTI010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242843"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VN039_12420"
     CDS             complement(<1..318)
                     /locus_tag="VN039_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWV46800.1"
                     /translation="MKIIAKFAFACAVSLAALSATAATAATAATAETAADKFVVGYAN
                     MADTDVFVMARKNAFIEAAKSDPAVTVNFSDANNDVSKQLDQIDNFIAQKVNAIVVVP
                     VDYQ"
     gene            complement(409..1419)
                     /locus_tag="VN039_12425"
     CDS             complement(409..1419)
                     /locus_tag="VN039_12425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV46801.1"
                     /translation="MSIDPVAGAEKREGRVLRGLREMSFSQIYRKYGTVLIFVGICIL
                     ASMLSPTFLTEANLTNVLRQVVVVSLLACGITFVIILGHIDVSLGSVLALCGVIAASV
                     MSMTGSIVLAVAAGLVVGVITGTINGFVITFFRIPSFIMTLAMTTVARGTVLLYTGGS
                     PVTGLGGFKIIGQGSLGPVPISVLILAVVVVLSWLLLNKTKFGRYVYAVGGNQRAALA
                     SGINPDSIVIKAFIFNGVLCAVAGIVLMSRINSGQPAGGVGYEFDAITAVVVGGTSLM
                     GGTGTITGTIIGSMIIGVINNILNLMNVSSYWQQIIKGLIIAIAVILDVWTKSARGKK
                     KA"
     gene            complement(1419..>2873)
                     /locus_tag="VN039_12430"
     CDS             complement(1419..>2873)
                     /locus_tag="VN039_12430"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWV46802.1"
                     /translation="VLALDEMNLQVRAGTVHVLVGENGAGKSTLMKILSGMYAIDGGE
                     IYFRGEKLDHQNAAAALDRGISMIHQELSPVLDMTIAENIFLGREPVRAETGLLSKFV
                     DFDRMNNETKVLLARVGLRYSPEMKMRELSIATMQLVEIVKAISREASLVIMDEPTSA
                     ISDTEVAMLFRQIAELKAAGVAIIYITHKMDEIFQIADDITVMRDGQYIAAGPASEYN
                     TGTLISQMVGRTISEVFPKEEVPIGEVVLSVENLTRHDVFENVSFNVRAGEIVGLSGL
                     IGAGRTEVARVIFGLDGADAGVVRLNGAALKLGSPRDAIASGIAMVSEDRKSEGLVLC
                     RSVGENISLANLQKFTSRLFISKRQEESAAQRMIKMLQIKTPNTAMTVENLSGGNQQK
                     IVLAKWLLGDLKLLILDEPTRGIDVGSKSEIHKLMTEFARQGLAIIMISSELPEILGM
                     SDRVVVMSGGRVTGELTRSEATQESIMRLATGGH"
BASE COUNT          624 a          819 c          806 g          624 t
ORIGIN      
        1 ctgatagtcg accggcacga caacgatggc attgaccttc tgcgcgatga agttatcgat
       61 ctggtcgagc tgcttgctga catcgttatt cgcgtcagag aaattgaccg tcactgccgg
      121 gtcagatttg gcagcctcga tgaaggcgtt cttacgtgcc atgacgaaca cgtcggtgtc
      181 ggccatgttg gcataaccga cgacaaactt gtcggctgct gtttcggcgg ttgcggcggt
      241 tgcggcggtt gcggcggttg cggaaagcgc ggccagagag actgcgcagg cgaaagcaaa
      301 ttttgcgata attttcatgg tttcctccca aagggtgtgt gttggtgcag gggcggaaat
      361 cccgccgaca cagagttatt tccgggaccg aaggtccctg gaacgggttt atgccttctt
      421 cttgccgcgg gctgatttcg tccaaacatc aaggatcacc gcgatcgcaa tgatcagacc
      481 cttaatgatc tgctgccagt aggagctgac attcatgaga ttgaggatgt tgttaataac
      541 gccgatgatc atggagccaa tgatcgtacc ggtgatggtt ccggtccccc ccatcagact
      601 ggtaccaccg acaaccacgg ccgtgatcgc gtcaaactca tagccaacgc cgcctgcggg
      661 ctggccggaa ttgatgcgcg acatcagaac aatgccggcg acggcacaga ggacgccatt
      721 gaaaatgaac gccttgatga cgatgctgtc ggggttgatt ccagaggcaa gtgcggcgcg
      781 ctgattcccg ccgacggcat agacgtatcg accgaactta gttttgttga gcagaagcca
      841 cgacaggacg accactaccg ccagaatgag cacggagatg gggactgggc ctagtgaacc
      901 ctgtcctatg atcttgaagc caccgaggcc agttacgggc gatccgcccg tatagagcag
      961 tacggttcca cgtgcgaccg tcgtcatggc aagcgtcata atgaacgagg ggattcggaa
     1021 gaacgtgatt acgaagccgt tgatcgtgcc agttatgaca cccacgacaa gaccggctgc
     1081 caccgcaagt acgatgctgc cggtcatcga catcacgctt gccgcgatta ccccgcaaag
     1141 agcaagaacc gagccgaggg aaacgtcaat atgccccagg atgatgacga aggtgatgcc
     1201 acaggccaaa agactgacaa cgaccacctg tctcagaacg ttagtgagat ttgcttccgt
     1261 caaaaacgtc gggctcaaca tcgaggccaa gatgcagatg ccgacgaaga tcaggacggt
     1321 gccgtacttg cggtagatct gggagaagct catctcccgt agaccccgca acacccgtcc
     1381 ctcccgcttt tctgctcctg ctactggatc tatactcatc aatgtcctcc cgtggcgagg
     1441 cgcatgatgc tttcctgcgt cgcctcgctc cttgtaagtt caccggtcac ccggcctccg
     1501 ctcatcacca ccacgcggtc actcatcccg agaatttcgg gcagctcgga tgagatcatg
     1561 atgatcgcga gcccttggcg ggcgaattcg gtcatgagct tgtgaatttc cgatttggat
     1621 cccacgtcga tcccgcgcgt aggctcatcg agaatgagaa gcttcagatc gcccagcagc
     1681 catttcgcaa gaacgatctt ttgctggttg ccgccgctca agttctccac agtcatcgct
     1741 gtgttgggcg ttttgatctg cagcatcttg atcatgcgct gcgcagcgct ttcctcctgc
     1801 cgtttgctga tgaacagccg cgaggtgaac ttctggaggt ttgccaaaga gatattttcg
     1861 cccacggacc ggcacagaac caggccctcc gacttgcgat cctcggacac catggcaatg
     1921 ccgctggcga tcgcgtccct cggcgagcca agcttcaaag cagcgccgtt cagtcggacc
     1981 actccggcgt cggcgccatc aagtccaaaa atcacgcgtg cgacctcggt tcggccggca
     2041 cctatgagac ccgataagcc tacaatctcg ccagcacgaa cattgaagct cacgttttcg
     2101 aagacgtcgt ggcgggtcag gttttcgacc gacaggacga cctcaccgat cggcacttct
     2161 tctttgggaa atacctcgga aatcgtccgc ccgaccatct gagagataag cgtgcctgta
     2221 ttgtattcac tagccggtcc cgcggcgata tattgaccgt cgcgcatgac ggtaatgtcg
     2281 tcggctatct ggaagatctc atccatcttg tgcgtgatat agatgatggc gacaccagcg
     2341 gctttcagct cggcgatctg ccggaagagc atcgcgactt cggtgtcgct gattgctgat
     2401 gtcggctcgt ccatgatgac aagagaggct tcgcgcgaga ttgccttgac gatctcgacg
     2461 agttgcatgg tcgcgatcga cagttcgcgc attttcattt cggggctgta acgcaggcct
     2521 acgcgggcga gcaggacctt tgtttcgttg ttcatcctgt cgaagtcgac gaacttggac
     2581 agaagcccgg tttcggcgcg gacaggttcg cgaccgagaa agatgttttc ggcgatcgtc
     2641 atatcgagca ccgggctaag ctcctgatgt atcatggaaa tgccgcgatc gagtgccgct
     2701 gcagcattct gatgatccag cttttcgccc cgaaaataga tctcgccgcc atcgatcgcg
     2761 tacataccgc tcagaatctt cataagggtc gatttgcccg cgccgttctc gccaacgagc
     2821 acgtgcacgg tgccggcacg gacctggagg ttcatctcgt caagcgcgag cac
//
LOCUS       DATMTI010000782         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_283579, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000782 DATMTI010000000
VERSION     DATMTI010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283579"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VN039_12435"
     CDS             <1..840
                     /locus_tag="VN039_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV46803.1"
                     /translation="LVVATNTPINDMFVVHTKQASYTTYVIGAGVPKNSVPTALYWDQ
                     ADPYHYARIQKDASTYGHDVLIVGGEDHKTGQADDGDERCQRLERWARKRFPMMEQIK
                     YRWSGQVMEPMDGLALIGRNPGDASNVYVATGDSGMGMTHGMIAGMLIADLIQGRANV
                     WAELYDPSRKSLKSIGTFVHENVNVAAQYADWISSGDIESEDHIPKDTGAVLRNGAQK
                     MAVYRDEDGQLYRRSAVCPHLKCIVAWDSMEHTWNCPCHGSRFDAYGKVINGPAHSDL
                     SQI"
     gene            1165..1398
                     /locus_tag="VN039_12440"
     CDS             1165..1398
                     /locus_tag="VN039_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWV46804.1"
                     /translation="MNADTFKGKWNQFKGELKQQWGKFTDDDLMLIEGNYDKFKGKVQ
                     ERYGDRKEELETWAAKWYGDTAKQVGEAGSSRR"
BASE COUNT          399 a          398 c          423 g          333 t
ORIGIN      
        1 cttgtcgttg ccaccaacac acctattaac gatatgttcg tggtacacac caagcaagcg
       61 tcctatacca cctatgtgat cggagccggc gtgccgaaga attccgtgcc taccgccctg
      121 tattgggacc aagcagatcc ctaccactat gcgagaattc agaaggacgc gtcaacctat
      181 ggtcatgatg tgttgatcgt aggtggtgaa gaccacaaaa ccggccaggc ggacgatgga
      241 gacgagcgtt gtcaacgatt ggaacgctgg gcccgcaaac ggtttcccat gatggaacag
      301 ataaagtacc gatggtcggg gcaggtcatg gagccgatgg atggcctggc attaatcggt
      361 cgaaatcccg gtgatgcatc gaatgtctat gtcgccaccg gcgactccgg catgggaatg
      421 acccatggca tgattgccgg catgctcatt gcggatctca ttcaaggacg cgccaacgtc
      481 tgggccgagt tgtacgatcc ctcccgtaaa tcattgaaat cgatcggcac gtttgttcat
      541 gaaaacgtaa atgtcgcggc gcagtatgcg gactggattt cgtccggtga cattgaatca
      601 gaagatcaca ttcctaaaga caccggagcg gtacttcgga acggcgcgca gaagatggcc
      661 gtgtatcgcg atgaggacgg acagttatat cggcgctcag ccgtgtgtcc tcacctgaag
      721 tgtattgttg catgggattc catggagcac acctggaact gtccatgcca tgggtcccgc
      781 ttcgacgcgt atggaaaagt gataaatggg cccgcgcaca gcgatctttc tcaaatttag
      841 acgtccggtt gttacgatac tgatcttcat cattccatcg tcggaactct cttccgttcg
      901 ctgatcttcc ccggtcagcg aggctctgct gtatcgatca tcctagcatg tcgtcactcg
      961 accacctgct tagcgtgggg ccatatgtaa atttcacgta agcattttca gcaagctttc
     1021 accgcatcgg cttccttcca acccaaggaa cccatgtcgg atcggtttac tagggaacag
     1081 gctagtcgat gccggcctcg tcatgcgtta cacaatacat gggaatagga gagagaacag
     1141 ttaatcacca accaggaggc tgatatgaac gcggacacat tcaagggcaa atggaatcaa
     1201 ttcaagggag agctcaagca acaatggggc aagtttaccg atgatgatct gatgctgatc
     1261 gaagggaact atgacaaatt caaaggtaaa gtccaagaac gctatggcga ccgcaaggag
     1321 gagctcgaaa cttgggcggc caaatggtac ggcgacaccg caaagcaggt tggggaagcc
     1381 ggctcctccc gaagataacg gcccgactga caatcagagg tccggagtcc ggcaacaaga
     1441 ttaagaggac aagaaattcg atgacaatgg ccatatggtc acgatcggct taaacgttcg
     1501 cttctagcag ttatgccgtc ttccgcagag cccggacgcc gacggaaggc ctg
//
LOCUS       DATMTI010000783         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_411858, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000783 DATMTI010000000
VERSION     DATMTI010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411858"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..866)
                     /locus_tag="VN039_12445"
     CDS             complement(105..866)
                     /locus_tag="VN039_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV46805.1"
                     /translation="MKVLSIAFNTFRENLRDKLLYNLLIFALLMIGSSLILMRLTLGE
                     FHRLILDIGLGSINFFGVLIAIFVGIGLVSKEIEKKTIYTIVSKPVARYQFLLGKYLG
                     LSLTLLINTAIMAAGLLAVLYFQDVPVHAVLFKALGMIVLEFMVITAVALLFSTFSSA
                     TLSAIFTLAIYVIGHLTTDLKAFGQKMEGVGRSVLEGMYYILPNLDRFNLKGNVTHQL
                     DVPAGDLLMIAAYGLAYTAFLLMLASVIFQQRDFR"
     gene            complement(863..>1550)
                     /locus_tag="VN039_12450"
     CDS             complement(863..>1550)
                     /locus_tag="VN039_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV46806.1"
                     /translation="YFYDYLTGREFLRFYGHLFGLVGNTLDKRIDELLELVGMAHARD
                     LQLRKFSKGMLQRVGIAQALINDPELVILDEPMSGLDPIGRKEVRDLILRLKESGKTV
                     MFSSHILHDAELLCDRVAMIMKGKLVACGHVSELIAGDTTHEVEMVIDRLLPEALERI
                     RPLASKVVLQGERVMVVLSSQQAVDQALEVIRSAKAKLVALTPHKASLEDLFIREAKG
                     IQSAVEARVS"
BASE COUNT          399 a          453 c          367 g          331 t
ORIGIN      
        1 cctccctatt ccaccactga atcccgctgc taccatcctg attcagaatg agcggaaatt
       61 ggaaccggtt tctcgctgaa tgagcttcat cttgcaaacg gagactagcg aaagtcccgc
      121 tgttgaaaga tgaccgaggc gagcatcagc agaaaagctg tataggccaa tccataggcg
      181 gcgatcatca gcagatcacc ggccggcacg tcgagttggt gagtcacgtt ccccttgaga
      241 ttgaatcgat ccaggtttgg aaggatatag tacatgcctt ccaacacaga ccgccctaca
      301 ccttccattt tctgaccgaa cgctttcaaa tccgtcgtca gatggccgat cacatatatt
      361 gccagggtaa agattgcgct gagcgtcgcg cttgagaaag tcgaaaacag gagcgccacc
      421 gctgtgatga ccatgaattc cagcacaatc atcccgagcg ccttgaagag cacggcatga
      481 acaggaacat cctggaaata caacaccgcc agcaagcccg ccgccatgat cgccgtattg
      541 atcaacagcg tcagactgag tccgagatac ttccctaaga ggaactgata ccgcgcgacg
      601 ggcttggaca cgatcgtata gatcgttttc ttctcgattt ctttgctgac caagccgatg
      661 ccgacgaaga tcgcgatcaa tacgccgaag aagttgatgc tcccgagccc gatatccaag
      721 atcaagcgat gaaactctcc cagcgtcaat cgcatcaaga tcagagaact gccgatcatc
      781 aaaagcgcga agatcaacag attgtagagc agcttgtctc gaagattctc gcgaaacgtg
      841 ttgaatgcga tcgagaggac cttcatgaaa ccctcgcctc caccgccgat tgtatccctt
      901 tggcttcacg aataaacaga tcttcgagcg aggctttgtg cggcgtgagc gccaccagct
      961 tcgccttggc cgaccggatc acctcgagcg cctgatccac cgcctgctgg ctggacagca
     1021 ccaccatgac ccgctcccct tgtagaacga ctttactagc caaaggtctg atccgttcga
     1081 gcgcctcagg aagcagccgg tcgatcacca tctcgacttc atgggtcgtg tctcctgcga
     1141 tcaattccga tacatgcccg caggcgacca actttccctt catgatcata gccacacgat
     1201 cgcataggag ttcagcatcg tgcaggatat gggagctgaa catgaccgtc ttgccggact
     1261 ctttaagccg cagaatcagg tcacgtacct ctttcctccc gatcggatcc agtcccgaca
     1321 tgggttcatc caaaatgacc agttccggat cattaattaa ggcctgggca atccccaccc
     1381 gctgcaacat gcctttcgaa aattttcgca actggagatc acgggcatgc gccatgccga
     1441 caagttccaa caattcgtca atgcgcttgt ccaaagtgtt ccctacaagc ccgaagaggt
     1501 gcccgtagaa tcgcaaaaat tctcggccgg tgagatagtc atagaagtat
//
LOCUS       DATMTI010000784         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_283687, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000784 DATMTI010000000
VERSION     DATMTI010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283687"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..>2743
                     /locus_tag="VN039_12455"
     CDS             98..>2743
                     /locus_tag="VN039_12455"
                     /inference="COORDINATES: protein motif:HMM:NF018319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWV46807.1"
                     /translation="MTSSDLRLDLAVDQSNPNQSDLLIRVGTNDDAIQLDTFDRANAL
                     GPRTVESFRFSDGSALTYEQLLTRGFDLTGTDNDDQITGTNMVDRIVAGSGADVLRSG
                     LGDDTLDGGLGTDRLFGGQGNDTYLFGPGAGQDTIVEFQGSLDAIRMVPGVAPSDVVV
                     TRNNNDLVLSLTGGADRLTLSLYFLAPPFQIELVQFADGTVWDQAFIENLTRPVITGT
                     EGPDSLIGTSGDDRLVGLAGDDQLTGLAGSDLLDGGTGADELTGGFGDDKYIVDDSGD
                     AVTELTDEGIDTVQSSVTHTLEADVENLTLTGSAAIHGTGNALDNVLIGNSGVNVLSG
                     GVGNDTYVVGTGDTVIELADEGTDTVQTGTSSTLGANVENLTLTGSVSLIGVGNELEN
                     VLKADGSISVLAGGDGNDTYIIGPNGDDDILAETATGGIDTVIAGHDYRLPANIEQLT
                     LLDPRVPDFASFSLIPYGSSEQSVTGYGNDLANILIGGRASNVLDGGLGADTLIGGDG
                     NDTYVVDNVGDIVIEEADGGLDSVESTVSYSLSANVENLTLSGAASINGTGNALNNDV
                     RGNDASNVLDGGAGNDALLGFGGSDTYLFGRGSGLDTLFDSEAAGEIDTIQFDSMVTA
                     EDVEVYRNGFNLELAISGTRDELTLLSFFDSSEYDQKQVRFSDGTLWSSAELSARALI
                     GNVVTGSFESETILGSDGHDVLIGSAGSDVLMGGHGKDTLYGDLTFQPSSGPQVIGDD
                     TLIGGDGNDSLFDFRGNNLFDGGAGDDTLSLGTGVDRVLFGRGSGVDYVTLDNNRNDI
                     DVIEMAAEITPAEIMMTWRSPSVADILVSDSGDRLTVQLSTDSFAVGPETTQAIVRFA
                     DGTLWNLAWSFSNVGIPTATSSDDVL"
BASE COUNT          594 a          669 c          852 g          628 t
ORIGIN      
        1 gcatgattcc tactttttca acataggtga tggtatcgat acgatcattg ataacgcctc
       61 agttggtgct ggtaatgaag ttgtttttgg ccctggtgtg acatcttctg atttgcgtct
      121 tgatctggca gtcgaccaat ccaaccccaa tcagagcgat ctgctgattc gcgtcgggac
      181 gaatgacgat gccattcaac tggatacctt cgaccgcgcg aatgcattgg gcccgcgcac
      241 agtggagtcg ttccgcttta gcgacggaag tgctttgacg tacgaacaac tgctcaccag
      301 aggattcgat ttaacgggaa cagacaacga tgaccaaatt accggcacga acatggtgga
      361 tcggattgtt gccggtagcg gagccgacgt gcttcggagc ggactgggcg atgacacgct
      421 cgatggcggg ttgggtactg accggttgtt cggcggacag ggcaacgaca cgtatctctt
      481 tggaccaggg gccggtcaag acacgatagt agaatttcag ggcagcctgg atgcgattcg
      541 catggtaccc ggcgtggctc cctccgatgt tgtcgttact cggaacaata atgaccttgt
      601 cctgagcctc accggtggcg ccgaccggct gaccctgtcc ctctacttcc tcgcccctcc
      661 atttcagatc gagctggtgc aatttgccga cggcacggtt tgggatcagg ccttcattga
      721 gaatctcacg aggccggtca taaccgggac ggagggaccg gattccctca ttggcacgag
      781 tggtgacgat cgtttggttg gcctcgccgg agacgatcaa ctgaccgggt tagctggtag
      841 cgatttactt gatgggggga ccggagccga tgaactcacg ggcgggtttg gtgatgacaa
      901 atatatcgtg gatgattcag gagatgcggt tacggagcta acggatgagg gaatcgatac
      961 ggtccagagc agtgtcacac atacactgga agccgatgtc gagaatctta cattgacggg
     1021 gagtgcggcg attcatggca ccggcaatgc actcgacaat gtgttgatcg gcaatagcgg
     1081 cgttaatgtc ttgagcggcg gtgtaggcaa cgacacgtat gttgtgggca caggcgatac
     1141 ggttatcgaa ctggctgatg aaggcacgga cacagtgcag accggcacca gctcaacgct
     1201 tggtgccaat gtggagaacc tgacgctgac cggcagtgtt tcgttgatcg gagtgggaaa
     1261 tgaactggag aacgtgctga aggccgatgg gtcgatcagc gtgctggccg gtggtgatgg
     1321 caacgacacc tatatcatcg gaccgaacgg tgatgacgat atccttgctg aaaccgccac
     1381 cggcggcatt gatacggtga tcgccggcca cgattatcgc ctgccggcga acatcgaaca
     1441 gttgactctc cttgatccac gagtacctga cttcgcgagt ttttccctca tcccatacgg
     1501 ttcttccgaa cagtccgtga cgggatacgg gaacgatttg gcgaacatcc tcatcggcgg
     1561 acgagcgagc aatgtgctgg acggcggact cggcgccgat acgttgattg gtggcgacgg
     1621 caatgacacc tatgtcgtgg acaatgtcgg tgacatcgtc atcgaggagg ccgatggagg
     1681 tctcgacagt gtggagagca ctgtgagtta tagtctcagc gcgaatgtcg agaatctcac
     1741 gctgagtgga gccgcttcca tcaatggaac cggtaacgcg ctgaataacg atgtgcgagg
     1801 caacgacgcg tccaacgtgc tcgacggagg ggctgggaat gatgcactgc ttggattcgg
     1861 tgggtccgac acctatctct tcggccgggg ctctggcctc gatacgctgt tcgactcaga
     1921 ggcagccggt gaaatcgata cgattcagtt cgattcgatg gtgactgctg aggatgtcga
     1981 agtctaccgc aatggattca acctcgagct ggccatcagc gggaccaggg atgagctgac
     2041 gctcctgtcg ttctttgatt catccgaata cgatcagaag caggtgcgat tttccgatgg
     2101 cacgctgtgg agcagtgccg agctgagcgc gcgggcgttg attggcaatg ttgtcacagg
     2161 atcattcgaa agtgaaacca ttttgggaag cgacgggcat gacgtgttga tcggatcagc
     2221 cgggagcgat gtgttaatgg gaggacatgg caaggatacc ctgtacggag atctcacgtt
     2281 ccaaccatcg tctgggccgc aggtcattgg tgacgacacg ctgataggag gggacggtaa
     2341 cgacagcctc tttgatttca ggggaaataa tcttttcgat ggaggggcag gggacgatac
     2401 gctgagtctt gggacaggtg tcgatcgggt tctcttcggt cgaggatccg gcgtcgatta
     2461 cgtgacgctc gacaataacc ggaatgacat cgatgtcatc gagatggctg ccgagattac
     2521 tcccgctgag attatgatga cgtggcgctc tccttccgtt gccgacattc ttgtttctga
     2581 cagtggggat agactgactg tgcagctttc gacggattcg tttgcggtgg ggccggaaac
     2641 cacgcaagca attgtgcgat tcgccgatgg cactctgtgg aatttggctt ggtcattttc
     2701 gaacgtcggt attcccacgg ccacttcatc cgatgatgtg ctc
//
LOCUS       DATMTI010000785         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_707571, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000785 DATMTI010000000
VERSION     DATMTI010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707571"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VN039_12460"
     CDS             <1..354
                     /locus_tag="VN039_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46808.1"
                     /translation="GVLISRHKDGGKMIVHSSKLACPVRVNLNRSKDYQAIEGLCTIV
                     AHEGKDTGYAHWKCTGNLKECTGDFTFTGGSGGFTGMSGTTPFQTSIVFEQQEGKTGQ
                     AVGYAVWPNLTYTLP"
     gene            complement(474..1217)
                     /locus_tag="VN039_12465"
     CDS             complement(474..1217)
                     /locus_tag="VN039_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWV46809.1"
                     /translation="MSDRSESEARHGRMLSVTDGEGLSMSPTGIVFLFDVDNTLLDND
                     RVTEDLQQYLEHEVGRERQKRYWTIFEQLRVELGYADYLGALQRYRVEYPRDPHLLTV
                     SHFLINYPFADRLYPSALAVIEHVTRWGTVVILSDGDVVFQPRKIERSGLFKAVQGRV
                     LIYIHKERELDDVKQRYSAGHYVLIDDKLRILSAVKTSWGSRVTTVFPRQGHYALDRQ
                     ILARFPQADMTIARIGDLLDHDFSTLRLR"
     gene            complement(1214..2584)
                     /gene="zwf"
                     /locus_tag="VN039_12470"
     CDS             complement(1214..2584)
                     /gene="zwf"
                     /locus_tag="VN039_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWV46810.1"
                     /translation="MAKSDAIVFFGASGDLAYKKIFPALQAMARRGHLEMPVIGVART
                     EWSLEQLKGRVHESLTEHGGLDAQAFGKLSGLLRYVSGDYREPATFERLRTELGGAER
                     PLCYLAIPPSMFPAAIEGLAQSGCLRGARVVVEKPFGRDLASAQALNSTLHAHLSEQA
                     VFRIDHYLGKEPVQNLIYFRFANPFFEAGWNRRYIERVQITMAEQFGVAGRGRFYEEA
                     GAIRDVVQNHMLMVIALLAMEQPAGSGHESIRDERAKVLKAIRTLTPSDVVRGQFRGY
                     RREDGVASDSQVETFIALQVHIDNDRWASVPFYVRAGKCLPVTATEVLVEFKRPPRPV
                     LDETEPPPPNHCRFQLNPDVLIALGTKAKAPGELMKGERIELVARHCSGDEMGPYERL
                     LGDAITGDPTLFVREDGVEAAWRVVEPILGATTPLYEYEPNTWGPNEADRFIGDGGWH
                     NPKADT"
     gene            complement(2930..3934)
                     /locus_tag="VN039_12475"
     CDS             complement(2930..3934)
                     /locus_tag="VN039_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46811.1"
                     /translation="MGGTPARVGVTTISAIPMMEIKGGQQMDWSEPLDYQYLKAANPE
                     MPAVPIKTFSGELHRAGATRVIHFDHARDLKTDYPATSPNCLASYVRICAGESITTDI
                     EAASHLFYILRGEGRTDTSSGAVPWRKDDLITLPACDRITHTATGDTAMYWVNDSPLL
                     RYLGVQPARPTFHPVHYTAEWLTQETRKAADDPDAASSNRIGILLANSSCPLTRTVTP
                     TLWSLLEIVPAGAVLPPHRHNSVALDLAIRGCGRGYTLMGRELDDENHIKDSVRVDWM
                     DASAFTTPPMLWHEHHNEGDEEAWLLPIQDAGLVTYDRILDIRFAAGGPPRNTGVTAF
                     "
     gene            complement(3971..>4373)
                     /locus_tag="VN039_12480"
     CDS             complement(3971..>4373)
                     /locus_tag="VN039_12480"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HWV46812.1"
                     /translation="FAGNGEIMTEAIGRTRWAIAEGYIPESSHGPEPQFLSHETACFL
                     NASEHDAHVTITIFYSDRDPVGPYRITVPARRTQHVRFNNLTDPEPIPRGTDYASLIE
                     SDVPVVVQHTRLDSRQAENALLSTIAFSADE"
BASE COUNT          908 a         1305 c         1249 g          911 t
ORIGIN      
        1 ggggtgctga tttctcgcca taaggacgga ggcaagatga tcgtccattc atcgaaactg
       61 gcctgtccgg tgcgtgtgaa tctcaataga agcaaagatt atcaggcgat agaaggcctc
      121 tgcacgatcg tggcgcatga aggcaaagac accggatatg ctcactggaa gtgcactgga
      181 aatttgaaag aatgcacggg cgatttcacg tttacgggcg ggtccggagg attcaccgga
      241 atgtcaggga caactccatt ccagactagt attgtttttg agcaacagga agggaaaacc
      301 ggtcaagcgg tcggatatgc ggtctggccg aatctgacct atacgttgcc ttagcgcgca
      361 ttattcacga acgcttctgc tgtaatcgct gattcaccgg ctacgacaag tctggacgcg
      421 atccaagagc gtccagatag gcgggcgcgc cgctcggtcg gtcgacatac ttttcacctg
      481 agtcgcagtg tcgagaaatc atggtcgagc agatctccga tgcgagcgat ggtcatgtcc
      541 gcctgtggaa aacgagccag tatctgccgg tccagcgcat agtggccctg gcggggaaac
      601 acagtggtca cgcgtgaacc ccagctggtc tttacggccg agagaatacg gagcttgtcg
      661 tcgatcaaca catagtgccc ggctgagtag cgctgcttca catcgtccag ctcccgttcc
      721 ttatgaatgt agatcaacac gcgcccctgt acggctttga ataaaccgga tcgttcgatc
      781 tttcttggct gaaacaccac gtcaccgtcc gagaggatca ccacagtgcc ccaccgtgtg
      841 acgtgttcga tcaccgcaag cgcgctcggg tacagccggt cggcgaacgg ataattgatc
      901 agaaaatgag agaccgtcag caaatgcgga tcacgggggt actcgacacg ataccgttga
      961 agggccccta aataatcggc atagcccaac tcgacccgca gctgttcgaa gatcgtccaa
     1021 tatcgtttct gacgctcacg gccgacttcg tgctcaagat attgttgcaa gtcctcggtc
     1081 acccgatcgt tgtccagcaa cgtattgtcc acgtcgaaga ggaagacgat gccggtcggc
     1141 gacatggaaa gcccttctcc atcggtgacg ctcagcatcc tcccatgcct ggcctctgac
     1201 tcgctccgat cgctcacgtg tccgccttcg gattatgcca accgccgtct ccgatgaatc
     1261 gatccgcttc gttgggcccc caggtgttcg gctcatattc atataatggt gtcgtcgcgc
     1321 ccagaatcgg ctctacgacg cgccatgcgg cttccacgcc gtcttctcgg acaaagagag
     1381 tcgggtcgcc cgtgatggca tccccgagga ggcgctcata gggacccatc tcgtcgccgg
     1441 aacaatgacg cgcgaccaac tcgatccgtt ctcctttcat gagttccccg ggagccttcg
     1501 ccttggtgcc gagggcgatc agcacgtcgg ggttgagctg aaaacgacaa tggttcggcg
     1561 gcggcggttc cgtctcgtcc aggacggggc ggggcggccg cttgaactcc acgaggacct
     1621 cggtggcggt cacgggcaga cacttgccgg cccgcacata aaacggcacg ctcgcccacc
     1681 gatcgttgtc gatgtgaacc tggagcgcga tgaaggtttc gacctgcgaa tcggacgcga
     1741 cgccgtcctc gcgacggtag ccacggaact gaccccgcac gacatccgag ggggtaagcg
     1801 tccgaatggc cttcaagact ttggcccgct catctcgaat cgactcatga ccgctcccgg
     1861 ccggttgctc catggccaac aatgcgatca ccatcaacat gtggttttgc accacatccc
     1921 gaatcgctcc cgcttcctca tagaagcggc cacgacctgc gacgccgaac tgttcggcca
     1981 tcgtgatctg cacgcgttcg atgtaacggc gattccagcc ggcttcgaag aagggattgg
     2041 cgaaacgaaa gtagatgaga ttctggaccg gctcctttcc aaggtagtgg tcgatacgga
     2101 agacagcttg ttccgataag tgggcatgaa gcgtgctatt cagagcctga gctgaggcta
     2161 aatctcgccc gaagggcttc tcgaccacca cgcgcgcgcc cctcaggcac ccggactgcg
     2221 ccagcccttc gatcgcggcg gggaacatgc tcggaggtat ggccaaatag cacagtgggc
     2281 gctcggcgcc cccgagttcc gtgcgcaatc gttcgaacgt cgccggctcg cgataatcgc
     2341 cgctgacata ccggaggagc cccgacaact tgccgaaggc ctgagcatcc agtccgccgt
     2401 gctccgtcag actctcatgc acccggcctt tcaattgctc caggctccat tcagtccttg
     2461 caaccccgat gaccggcatc tcgagatggc cgcgccgcgc catggcctgc agggccggaa
     2521 agatcttctt gtaggcaaga tcgccgctgg ccccgaagaa gacgatcgcg tcggactttg
     2581 ccatacccat gatcatcctc catttccccg gccgactgtc ccatgctata ggcctacatt
     2641 cacggattca attattttgg agagaaggcg gcagggccta tcgttacgcg gcctatttct
     2701 ttgaacaagg tttttcctga gagcttgcgg agtgtctggt ccatcgattt aaagagctgc
     2761 cgggtttgcg ctttgccggc tcggcccatt gctgcgcgcg atcccatcgt ttcatattcc
     2821 catggcctca tgatgctgtc acacacaatc ccgaagagct aatgtcaagc gccggcggtc
     2881 aatctcatat cactacgcct gccaccttgg acatgccgcc tccacgctat caaaacgcgg
     2941 tgactccggt gttccgtgga ggaccgcctg cggcgaaacg gatatcgagg atgcgatcat
     3001 aggtgacgag ccccgcgtct tggatcggga gcagccaggc ctcttcatcg ccctcgttgt
     3061 gatgctcatg ccacagcatc ggaggcgtcg tgaaagccga ggcgtccatc caatcgacac
     3121 gcacggagtc cttgatgtgg ttctcatcat cgagttcgcg acccatcagg gtataacctc
     3181 gaccacatcc gcgaatggcg aggtcgagcg cgaccgaatt atgtcgatgg ggtggcagga
     3241 ccgcaccggc aggcacgatt tctaacaatg accacagcgt cggggtcacc gtacgggtga
     3301 gtgggcacga agaattggcg agcaaaatgc cgatgcggtt cgaagacgcg gcgtccggat
     3361 cgtcggcggc cttccgcgtc tcctgtgtca gccactcggc cgtgtagtgt acggggtgaa
     3421 aggtggggcg ggccggctga acacccagat atcgcagcag gggggaatca ttgacccaat
     3481 acatcgccgt gtcccctgtg gccgtgtgcg tgattcggtc acacgccggt agcgtgataa
     3541 ggtcgtcctt ccgccagggc acggccccag acgacgtgtc tgttcggcct tcaccgcgta
     3601 agatgtaaaa aagatgagag gccgcttcga tgtccgtggt gatcgactcg ccggcgcaga
     3661 tgcgtacata gctggcaaga cagttaggac tcgtcgccgg atagtcagtc ttcaggtccc
     3721 tcgcgtgatc aaaatgaatg acgcgtgtcg ctcccgcacg gtggagctcg ccggaaaagg
     3781 ttttaatcgg gaccgcgggc atttcagggt tggccgcctt cagatattga taatccaatg
     3841 gttcactcca gtccatctgt tgccctcctt ttatctccat catcgggata gccgaaatcg
     3901 tcgtcacccc cacgcgagca ggtgttccac ccacatatcc gccaccgtat gtgccgtccg
     3961 ggaccccgcc ttattcgtct gccgaaaatg cgatggtgct gagcaaggca ttttccgcct
     4021 ggcgggaatc caagcgggta tgctggacga ccaccggcac atcggactca atgaggctgg
     4081 catagtctgt cccgcgcgga atcggctccg gatcggtgag attgttgaac cgaacgtgct
     4141 gcgtgcgacg cgccggcacc gtgatgcgat agggtcccac cgggtcacga tccgaataaa
     4201 aaatcgtgat cgtcacatgc gcgtcatgct ccgaggcatt tagaaagcag gcggtttcat
     4261 gactcagaaa ctgcggttcc ggtccatggc tgctttccgg gatgtaccct tctgcaatgg
     4321 cccatcgtgt tcgtccaatg gcttccgtca taatttctcc attccccgcg aat
//
LOCUS       DATMTI010000786         1963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_71416, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000786 DATMTI010000000
VERSION     DATMTI010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71416"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VN039_12485"
     CDS             <1..1165
                     /locus_tag="VN039_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWV46813.1"
                     /translation="LNFGFGVVTGIPLEFQFGTNWARFSTFAGGVIGQTLAMEGVFAF
                     FLESSFLGILLFRKRFSRRVQWFAALMLFVGSWLSGYFILATNAWMQHPVAYQVGDDG
                     RVFVNSLTGLLTNPWLFWQFAHNMTAAVVTASCVIAAVGAYYLLSGQYLSHAKTFLQT
                     GVVAGAIASALMIFPTGHGNAKQVFEHQPVKGAAFEGLFTTERGAGLYLVGQPNLETM
                     TIDNPIEIPKALSFLVYKDLYAKVKGLDAFPRDEWPDNLPLLYYAYHVMAGLGTIFAA
                     IFGVALLSLWRGRLFNMKWLLWLLMLSAPFPYIATSAGWMTAELGRQPWLVYGLLRTA
                     HGTSPLVHSGNALFTLIGFLGLYLLLGLLFVFLLGNTIRRGPGSIDSQTTYLV"
     gene            1181..>1963
                     /gene="cydB"
                     /locus_tag="VN039_12490"
     CDS             1181..>1963
                     /gene="cydB"
                     /locus_tag="VN039_12490"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWV46814.1"
                     /translation="METLWFIVVTLMLAVFIVLDGFDFGVGMVYPFVVRTEAERRTVL
                     ASIGPLWNANEVWLIAAGAVLFFAYPKAYAAGFSGFYLALILVLWLLIFRGLALELRS
                     HVEHVLWKQAWDTAFTTASLLLAFVFGAALGNLIRGVPLNRDGYFFVPFWTDFLPGPE
                     PGILDWFTVLLGLTSVSILAFHGSNYLVMKTEGALYKRAVSTACVMGWVSAGCTVLTV
                     TAVPFVQPSLRLNFAGHPMEYLVPLSGIFALGSSLYLRQRQCD"
BASE COUNT          328 a          552 c          563 g          520 t
ORIGIN      
        1 cctaaatttt ggcttcggcg tcgtgaccgg gatcccgctc gaatttcagt ttggaaccaa
       61 ctgggcgagg ttttctacct tcgcgggtgg agtgatcggc caaactctcg ccatggaagg
      121 agtcttcgca tttttcttgg aatcatcgtt tctcggcatc ctgctgttca ggaaacgatt
      181 cagtcgtcgg gtgcaatggt tcgccgctct gatgctcttt gtcggttcgt ggttatccgg
      241 ctacttcatc ctggcgacga atgcctggat gcagcatccc gtcgcgtatc aagtgggaga
      301 cgatggccgg gtgttcgtca acagcctcac cgggctgctc acgaatccct ggttattctg
      361 gcaattcgcg cataacatga cagcggccgt cgtcacggcg tcctgtgtga tcgccgcagt
      421 gggtgcgtac tatctgttat ccggtcaata tctatcccat gcgaagactt tcctacagac
      481 cggtgttgtc gccggcgcaa tcgcttctgc cctgatgatt ttccccacgg gacacggcaa
      541 cgccaagcaa gtcttcgaac atcaacctgt caagggggcg gcgtttgagg ggctcttcac
      601 gacggagaga ggagccggtc tctatctggt gggacaaccc aatcttgaaa cgatgaccat
      661 cgacaacccg attgagatcc ctaaggcgct cagctttctc gtgtataaag acctctacgc
      721 caaggtcaag gggctcgacg cgtttccgcg cgacgagtgg ccggataatc ttccgctcct
      781 ctactacgca tatcacgtca tggccggatt ggggaccatc ttcgccgcca tctttggcgt
      841 ggccctgctc tcgctttggc gtggccggtt gttcaacatg aagtggctat tgtggttact
      901 gatgctctca gcgccattcc cctacatcgc cacgtcggcc ggctggatga ccgccgaact
      961 gggacggcag ccctggctcg tgtatggcct gttgcgcacc gcccatggca catctccgct
     1021 tgttcattca gggaatgcct tatttacctt gattggattc ttgggactgt atttgctgct
     1081 cggcttgctg tttgtgtttc ttctcggcaa cacgattcga cgcggacctg gctcgatcga
     1141 ctcgcaaacg acgtatctcg tgtgaacgaa ggggctgttg atggaaacct tgtggtttat
     1201 cgtcgtcaca ctgatgttgg ccgtcttcat tgtcctggat ggtttcgact ttggtgtcgg
     1261 catggtctac ccgttcgtgg ttcggaccga agcggaacgc cgaacggtgc tcgcctccat
     1321 cgggccactg tggaacgcaa acgaagtctg gttgatcgcc gccggcgcgg tcctcttctt
     1381 tgcctatcct aaggcctacg ccgccggatt cagcgggttc tatttggcgc tgatccttgt
     1441 gctatggctg cttatctttc gcggtctcgc gctggaattg cgatcgcacg tggagcatgt
     1501 gttgtggaaa caggcgtggg atacggcctt tacgacggcc agtttgctct tggcttttgt
     1561 cttcggcgcg gcgctgggca atctgattcg tggtgtgccc ttaaatcgtg acgggtattt
     1621 cttcgtcccg ttttggaccg actttctgcc tggaccggaa ccgggcatcc tggactggtt
     1681 cacggtgctg ctggggctga cgagtgtgtc gatcctagcc ttccacggat cgaattatct
     1741 cgtcatgaaa acagaaggcg ccctgtataa gcgggccgtc tcgaccgcct gcgtcatggg
     1801 gtgggtttcg gctgggtgca cggtgcttac cgtgactgcc gtccctttcg tccagccaag
     1861 cttgcggctc aacttcgccg gccatccgat ggaatatctt gtcccattga gcggcatatt
     1921 cgcgcttgga tccagtctgt acctccggca gcgacaatgt gac
//
LOCUS       DATMTI010000787         1640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_707599, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000787 DATMTI010000000
VERSION     DATMTI010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707599"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1640)
                     /locus_tag="VN039_12495"
     CDS             complement(<1..>1640)
                     /locus_tag="VN039_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWV46815.1"
                     /translation="IVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVPSRIGT
                     LLDMSLKQLEKILYFESYVCVDPGSTDLTEKELVPEDKLRTLVSEFGASGFKVGIGAE
                     AIRDLLRKIDINALWDDLQVKAKASTSAAMKKKYAKRLKVLEAFRKSGNKPEWMIMDV
                     IPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLMELKAPGVIIRNEMRML
                     QEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRTVIVV
                     GPELRLHQCGLPKKMALELFKPFIFHKLEARGAATTIKSAKRLVEKERPEVWDVLDEV
                     IREHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAVHVPLS
                     VEAQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERLGAKGEGKVFGSSE
                     EVRIAFDAREVEEHARIKVRLAGSLVQTTVGRVLLSEILPPGLPFANANKLMTKKEMT
                     KLIDAVYRQTGHRDTVEFLDKIKDIGFNYATRAGLSIC"
BASE COUNT          355 a          515 c          401 g          369 t
ORIGIN      
        1 atgcagatcg acaagccggc tctggtcgca tagttaaagc cgatgtcttt gatcttatcc
       61 aaaaactcga ccgtgtctcg atgaccagtc tgtcgataca cggcgtcaat gagtttcgtc
      121 atctcctttt tagtcatgag cttgttcgca ttcgcgaagg gcaggcccgg agggaggatt
      181 tcggacagca agacacgacc aacggttgtc tgcaccagcg aaccagcaag gcgcaccttg
      241 atccgcgcat gctcctccac ttctctcgcg tcaaaggcga tcctgacttc ttcggaagac
      301 ccaaacactt tcccttcgcc cttggcgcct agccgctctt tcgtcaacca ataacagccc
      361 aacaccatat cttgcgacgg caccgcgatc ggcttgccgt tggccgggga gagaatgttg
      421 ttgatggaca tcatgagaac ccgcgcttca acctgcgctt ccacagacag cgggacgtgc
      481 accgccattt gatccccatc gaagtccgcg ttgaacgccg cacagacgag cgggtgcaat
      541 cggatcgcct tgccctccac caacacagga tcgaatgcct ggatgcccaa ccggtggagc
      601 gtcggggctc gattcagcaa taccggatgc tctcggatca cttcatcaag gacatcccaa
      661 acctcaggcc gttctttttc gaccagccgt ttggcgctct taatggtggt ggccgcccct
      721 ctggcttcca gcttgtggaa gatgaacggc ttgaacaatt ccagcgccat tttcttcggc
      781 aacccgcact ggtggaggcg caactccgga ccgacgacga tcacggtacg gccggaataa
      841 tcgacacgct tcccgagcag gttctgccga aaccgcccct gttttccctt cagcatgtcg
      901 ctcaacgatt tcaacggacg tttgttcggg ccacgaatcg cgcgcccgcg gcgaccgttg
      961 tcgaagagag catccacggc ttcctgcaac atccgcatct cgttcctgat gatgacaccg
     1021 ggcgccttca gttccatcaa tcgtttcaac cgattatttc gattgatcac gcggcggtag
     1081 agatcgttca gatcggacgt ggcaaaccgt cctccatcga gcggaaccaa aggacgcaac
     1141 tccggcggca ggaccggaat gacatccatg atcatccatt caggcttatt cccggacttg
     1201 cggaacgcct ccagcacttt caaccgtttg gcgtacttct tcttcatggc cgccgaggtc
     1261 gacgcttttg cctttacttg taggtcatcc cacagcgcat tgatgtcgat cttcctcagt
     1321 aagtcgcgga tggcctcagc gccgattccc actttaaatc cactcgcccc gaactcagaa
     1381 acaagggtac gcagtttatc ttccggcacc aactcttttt ccgtcagatc ggttgagccg
     1441 ggatccacac acacataact ttcaaaatag aggatttttt cgagctgctt gaggctcata
     1501 tcgagcaggg ttccgatccg actcgggacc cccttgagaa accaaatatg ggcgacaggc
     1561 gcagccagct cgatatgccc catccgttcg cgccgaacct tcgattgaat cacctcgaca
     1621 ccgcatttgt cgcacacgat
//
LOCUS       DATMTI010000788         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_666010, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000788 DATMTI010000000
VERSION     DATMTI010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666010"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VN039_12500"
     CDS             <1..888
                     /locus_tag="VN039_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWV46816.1"
                     /translation="LITIKDIEKRIKYPNACKDSHGRLRVGAAVGVGQDTEERVTLLK
                     KSGVDLVVIDTAHGHSQAVLDRAKMIKKLYPDLELVAGNIGTAEAAKDLLKVGVDAVK
                     VGVGPGSICTTRIVSGAGMPQLTAIADCAKVLNGSGVPVIADGGIKFSGDITKALAAG
                     ASSVMLGGLFAGTEESPGETVLYQARTYKVYRGMGSIGAMERGGGDRYGQGERPAQKL
                     VPEGIEGRVPYKGSLAAVVYQLVGGVRSGMGYCGCKTIADLQRNATFIRQSVAGLRES
                     HVHDVIITKEAPNYRMDWE"
     gene            987..2543
                     /gene="guaA"
                     /locus_tag="VN039_12505"
     CDS             987..2543
                     /gene="guaA"
                     /locus_tag="VN039_12505"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWV46817.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAQVYSQILPCTVPLATI
                     LAYRPQGIVLSGGPSSVYEKNAPIIAKEVFDQNIPILGICYGMQLVTHLSGGTVAKST
                     HREYGGADLTIDDAGDLFKGVGTDRSTAVWMSHGDRIERMPPGFRSIAHTNNSPVAAM
                     KRDDPKRRIYCLQFHPEVAHTPEGTKILRNFVYGICGCKPTWTMQSYVETAVGQIREQ
                     VGKERVICALSGGVDSSVAAALTHRAIGDQLTCIFVDNGVLRAGERDQVQKTFASQLH
                     LKLRIVDGTKQFLADLKNVTDPERKRKIIGRRFIKHFEAESKKLKGIKYLVQGTLYPD
                     VIESISFKGPSATIKTHHNVGGLPARMKLKLIEPLRELFKDEVRVLGTELGLPDEIIW
                     RQPFPGPGLAIRVLGAVTPERLAILRAAEAIVDQEIRSAGLYREIWQAFAVLLPIRTV
                     GVMGDQRTYENVIAIRAVTSVDGMTADWAKIPNEVLGRMSNRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
BASE COUNT          648 a          714 c          870 g          595 t
ORIGIN      
        1 ctcattacca tcaaggacat cgaaaagcgg atcaaatatc ccaatgcgtg caaggacagc
       61 catgggcgtt tgcgggtcgg ggcggcagtc ggagtcgggc aagatacgga agagcgtgtg
      121 accctgctca agaaatccgg cgtggatctt gtggtgatcg acacggcgca cggccattcg
      181 caagccgtgt tggacagggc gaagatgatc aaaaagctgt atccggatct cgagctggtt
      241 gcgggaaaca ttggaaccgc agaagcggcc aaagacctcc tcaaagtcgg tgtcgatgcg
      301 gtcaaggtcg gggtcggacc gggatcgatc tgcacgacac ggattgtgtc gggagccggg
      361 atgccgcagc tgaccgccat cgcggattgc gcaaaagttt tgaatgggag cggtgtgccg
      421 gtcattgccg atggaggtat caaattttcc ggcgatatca caaaggcctt ggccgccggg
      481 gcgtcttccg tgatgctcgg cgggctcttt gccggaacgg aagagtctcc cggtgagacg
      541 gtgctgtatc aagccagaac ctacaaggtc tatcgcggta tgggttccat cggggcaatg
      601 gagcgaggag gcggggatcg gtacgggcaa ggagaacgcc cggctcagaa gctggttccc
      661 gaagggatcg aaggccgtgt tccctataaa ggctcgttgg ccgccgtggt gtatcagttg
      721 gtcggcggtg tgagatcggg gatgggatat tgcggctgta aaacgattgc cgatttgcaa
      781 cggaacgcca cattcatccg acagtccgtt gccggcctcc gtgagagcca tgtgcatgac
      841 gtgatcatta ccaaagaggc gccgaactat cggatggatt gggaatgatt ttccgtgagg
      901 ggttaggcgt caggcgttag gcgagttcta actccgaagc aagcatctgc acttcacgcc
      961 tcacccctta cgcctcacgg gattcgatgg aactctggca cgatagaatt ctggtccttg
     1021 actttgggtc ccagtacacg cagctgatcg cccgccgcat tcgcgaagcg caggtctatt
     1081 cgcaaattct tccctgtacg gtcccattag cgacgattct cgcctatcgg cctcaaggca
     1141 ttgtcctttc cggcggcccg tccagtgttt atgagaagaa cgcgccgatt atcgccaaag
     1201 aggtgttcga tcagaacatt ccgatcctcg gtatttgcta cggcatgcag ttggtgaccc
     1261 atctttccgg ggggacggtt gcgaagtcga cccatcgtga gtatggcgga gccgatctga
     1321 cgatcgatga tgccggggat ctctttaaag gagtcggaac cgatcggtcg actgccgtgt
     1381 ggatgtctca cggtgatcgt atcgaacgga tgccgccggg atttcgatcg atcgcgcata
     1441 cgaataactc gcccgttgcg gcgatgaagc gggacgatcc caagcgccgg atttattgtc
     1501 tgcagtttca ccccgaagtg gcgcacacgc cggaaggaac gaaaattctc cgcaatttcg
     1561 tctatggaat ttgcggttgc aaaccgacct ggaccatgca gtcctacgtc gagaccgcgg
     1621 tcgggcaaat ccgtgaacag gtcggcaagg agcgagtcat ctgcgcgctg agcggcggcg
     1681 tggattcctc ggttgcggcg gcgctgacgc accgggcgat cggcgatcag ctcacgtgca
     1741 tcttcgtcga taatggcgtc ttgagggccg gtgaacgtga tcaagtacag aagaccttcg
     1801 cctcgcagtt gcatctcaaa ttgcgaattg tagacgggac caaacagttt ctcgccgatc
     1861 tgaagaacgt gacggatccg gagcgaaaac gaaagatcat cggccgccgg ttcatcaagc
     1921 acttcgaggc tgaatccaag aaactgaaag gcatcaagta tctcgtccaa ggcacgctct
     1981 accccgacgt gatcgaaagc atcagcttca agggcccatc ggctaccatc aagacgcatc
     2041 acaacgtcgg cggattgccg gcgcgcatga agctcaaact tatcgagccg cttcgcgaat
     2101 tattcaagga cgaagtgcgc gtgttgggaa cggagctggg attgccggat gagatcatct
     2161 ggcggcaacc gttcccgggt cccggattgg cgattcgcgt gctgggcgca gtcacaccgg
     2221 aacggttggc gattctgcgg gcagcggaag cgatcgtcga tcaggaaatc cgaagcgccg
     2281 gtctttatcg cgagatctgg caggcgtttg ccgtcttgtt gccgattcga accgtcggag
     2341 tgatgggtga tcagcggacc tacgagaatg tcatcgcgat ccgagctgtc accagtgtgg
     2401 acggcatgac cgccgattgg gcaaagatac cgaatgaggt tcttggccgc atgtcgaatc
     2461 gaatcatcaa cgaagtcaaa ggcgtgaatc gggtcgtgta cgacatcagc tcaaagccgc
     2521 cgagtaccat tgagtgggag tagaaatcct gaagtaaatt atgtagtcga aagctacatg
     2581 taaagatacg cgaagcagca tcaactcaaa gctgtagttt ctcgaagtga ccgtctccgg
     2641 attgtagtga cgcaagcgag gtgattattg tcggttcact ggttcaaatg ggaatgagta
     2701 aaccggcggc ggcactgaac gagagctgca gcgcgagtaa atgcgtttga ctacatcgaa
     2761 agctgatata ggggaacaat tcttacttag ctagggaagg tctaaatagt tcccaatttc
     2821 tgtcagt
//
LOCUS       DATMTI010000789         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_581031, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000789 DATMTI010000000
VERSION     DATMTI010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581031"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2365
                     /locus_tag="VN039_12510"
     CDS             <1..2365
                     /locus_tag="VN039_12510"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWV46818.1"
                     /translation="QGSENAYAFKAEIGKGEQVAWEGTFSLEPVESDGKLNLSGIKLQ
                     TLYQAVRDQFQFDVPQGILGLSASYHFDLRGQVPQATVKNGAVSIQNLALVERGGIEP
                     VVKIPAFGVEGIQLDLQKQTIDVSKVHSADARFEAWMDPDGTLNYQQLFTPVGGRGAE
                     EKLPAATAKPEKPAKPWSIAVDEIEIKNYGASFEDRTLERPGHVDVEAMNVTVKDIQL
                     PFKRPLPIDLSLKLNETGSIDVKGKVAVEPPAADTELKLEHIDIRPFQPYLDRFLNAD
                     VLDGAIDLNGTVHFAKEHPNEPMMRFQGNLAVNRLVVADRKDLDNVLTWKGLNVRQIA
                     LDVEPTAVKIEEVVWQEPSIRMVIDAEGQLNLSRLAVSPPPGDQTAAPKKPEGENSRA
                     AAKDPVSVTIDQVKLGKLAATFQDLSIEPHVRTSLTEFGGTIKGLSSKQLKKADVNLR
                     GKVGRAALLKIAGKINPLSEDAFTDLIVTLGAMDLTPVGPYSGKYAGYGLSKGKLSLD
                     LKYKVSQKVLEAENLVHVDQLTFGEKTNSPDATSLPVPLVVALLQDRKGLIEIDMPIR
                     GNLNDPDFKYGKVVISTLLNLLGKVVASPFALMGKLVPGGGGEEDLQFIEFQPGSDSL
                     ADSELTKLEALEKALDERAGLRLDIKGTTDSTLDAAALQKMKLRDQLFAMSGGAKPDQ
                     EELSPKEEQRLVEKLYAKLPPPDPSATPAEPTQPTVADMKRQLAAAIQISTSELETLA
                     HQRAEAIRHHLLESGKLTEERVALLDTGVAESDHEKVRTQLSLSAGP"
     gene            complement(2373..2738)
                     /locus_tag="VN039_12515"
     CDS             complement(2373..2738)
                     /locus_tag="VN039_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWV46819.1"
                     /translation="MAGMDTARTKLHISKDPMYLMLREGCIKEFNVKKAAGDKCDLRG
                     CDLRGLDLRGLDADGLDFSDCYFRQSDLRGVDLSKTKMDGASINACKISGVLFPAELS
                     ASEIELSLLHGTRMRYSVK"
     gene            2922..>3362
                     /locus_tag="VN039_12520"
     CDS             2922..>3362
                     /locus_tag="VN039_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWV46820.1"
                     /translation="MAIRLGDDAPNFTAETTEGTINFHEWLGNGWGILFSHPKDYTPV
                     CTTELGTVAKISPEFKKRGVKMIAVSVDPMDSHKGWINDINETQKTTMNYPIIADPDK
                     KVATLYDMIHPNAIDNMTVRSVFIIGPDKKVKLTLTYPASCGRNF"
BASE COUNT          817 a          867 c          986 g          692 t
ORIGIN      
        1 ccaaggaagc gagaatgcct atgctttcaa ggcggaaatt ggaaagggtg aacaagtggc
       61 gtgggaagga accttctcgc tggagccggt ggagtccgac ggcaagctga atttatccgg
      121 aatcaagctg cagacgttgt atcaagctgt gcgcgatcaa tttcagttcg atgtcccgca
      181 ggggatcctc ggtctatccg cgtcgtacca tttcgatctt cgcggtcagg ttcctcaggc
      241 aacggtcaag aatggagcgg tatcgattca gaatctcgct ctcgtggaac ggggagggat
      301 cgagccggtc gtgaaaattc ctgcttttgg tgtggaaggg attcagctgg atctgcaaaa
      361 gcagaccatc gatgtctcga aggtgcattc agccgatgcc cggttcgagg cctggatgga
      421 tcctgatggc acgctcaact atcaacagct cttcacaccg gtcggcggga ggggagcaga
      481 ggaaaaactt ccagccgcga ccgccaagcc ggaaaaaccg gcaaagccct ggtctatcgc
      541 tgtcgacgaa atcgaaatta agaactacgg cgcatcattc gaagaccgaa cccttgaacg
      601 tcctggacac gtggatgtgg aggcgatgaa tgtcacggta aaggatatcc agcttccgtt
      661 taaaagaccg cttcccatcg acctgtcatt gaaactgaat gagactgggt ccattgatgt
      721 gaagggaaag gtggcggttg aacctccggc agccgatacg gagctcaagc tggagcacat
      781 cgacatccgg ccgtttcaac cctatctgga tcggtttctc aacgccgatg tattggacgg
      841 tgcgatcgat ctgaacggaa cggtgcactt tgcgaaggag cacccgaacg aacccatgat
      901 gcgattccag ggcaaccttg cagtcaatcg gctggtggtc gcggatcgca aagatctcga
      961 caatgtgctg acatggaaag ggttgaacgt gcggcaaatt gctctggatg tcgagccgac
     1021 ggcggtgaag attgaggaag tcgtctggca agaaccttct atccggatgg tgatagatgc
     1081 tgaaggacaa ctcaatctct cgcgtctcgc cgtctcgcct ccgcccggtg atcagactgc
     1141 agctcccaaa aagcctgaag gcgagaattc gcgagctgcg gcaaaggatc ctgtgtctgt
     1201 caccatcgac caggtcaagc tggggaaatt ggccgcaacg tttcaggatt tgtctattga
     1261 accacacgtg agaaccagcc tcacggagtt cggcgggacc atcaaaggtc tgtcgtcgaa
     1321 gcagctgaag aaagccgacg tcaacctgag gggcaaagtt ggacgagccg ctctcctaaa
     1381 aatagccgga aagatcaacc cgctgagcga agatgccttc accgatctga tcgtgaccct
     1441 tggcgcgatg gatttgacac cggttggacc ttacagcggt aaatatgcgg gctatggact
     1501 gtcgaaggga aaattgtcgc tcgatttgaa gtacaaggta tctcagaaag tgttggaagc
     1561 ggaaaatctg gtgcatgtcg atcagctgac gttcggtgag aagacgaata gcccggatgc
     1621 cacgtcgctg cctgttccgc tggtcgtagc gctgctgcaa gatcggaagg gcctcatcga
     1681 aatcgatatg ccgattcgcg gcaatctcaa cgatccggat ttcaagtatg ggaaggtcgt
     1741 catctcgacg ttgctcaatc tgctcgggaa agtcgtcgct tcgccgtttg cgctcatggg
     1801 caagctcgtg cctggtggcg gtggcgagga agatcttcaa tttatcgaat ttcaaccagg
     1861 gagcgactcg ttggccgaca gtgaactgac taagctcgag gcattggaga aagcgttgga
     1921 tgagcgggcg ggactccggc tcgatatcaa gggaacgacc gattcgacgc tggatgcggc
     1981 ggcgcttcaa aagatgaagc tccgagatca gctgttcgcg atgagcggcg gggcaaaacc
     2041 cgatcaggaa gagctgtcgc cgaaagagga gcaacggttg gtggaaaaac tctatgcgaa
     2101 actgcctccc cctgatccat ccgcgacacc ggccgaaccc acgcagccga ccgtggcgga
     2161 catgaagcga caactcgcgg cggccatcca aatttccacg agcgaattag agacgctggc
     2221 tcaccagcgc gccgaggcga tccgccatca tcttctggag agcggaaagc tcacggagga
     2281 acgagtcgcg ctgctcgata cgggcgttgc tgagtctgat catgagaagg tgcggactca
     2341 gttgtcgctg tcggcaggac cgtgaacgac agttacttca cgctgtaccg catccgcgtc
     2401 ccgtggagaa gagacagctc aatttcagag gcgctcagct ccgcggggaa gagcacgccg
     2461 gaaatcttgc acgcattgat actggcaccg tccattttgg ttttgctcaa gtcgacgcca
     2521 cggagatcgg attgccggaa atagcagtcg ctgaaatcca gcccgtcggc atctaagcct
     2581 cgaaggtcga gaccgcgcag atcacatcca cggagatcgc acttgtcccc ggcggctttc
     2641 ttaacgttga actccttgat gcagccttca cgcagcatga ggtacatcgg gtcctttgag
     2701 atatggagtt ttgtccgggc tgtgtccatg ccggccatgt tcggctccgt tgaaaaggat
     2761 ttctgaagct tctatcggca tgagacgcga gaagcttgag ctagggggaa gcctcagcct
     2821 tgaccttgcg aatgcctctt gaactcgccc gtttggtcac agtattgtac ggtgatagta
     2881 gaaatcttgt cgtaggtgat cgcgatacaa ggaggtatcc catggcaata cgattgggcg
     2941 acgatgcgcc gaattttacg gcggaaacga cggagggtac gattaatttt cacgaatggc
     3001 tgggcaacgg ttgggggatc ctcttttcac accccaagga ctacaccccg gtgtgtacga
     3061 cggagctggg caccgtggcc aaaatctcgc cggagttcaa aaagcgaggc gtcaaaatga
     3121 ttgccgtcag cgtggatccg atggattccc acaaaggctg gatcaacgac atcaacgaaa
     3181 cgcaaaagac gacgatgaac taccccatca tcgccgatcc ggataagaag gtcgccacgc
     3241 tgtacgatat gattcatccc aacgccatcg acaacatgac ggtgcgatcg gtctttatta
     3301 tcggtccgga caagaaggtc aaactcacgt tgacctatcc agcttcctgc ggccgaaact
     3361 tc
//
LOCUS       DATMTI010000790         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_412117, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000790 DATMTI010000000
VERSION     DATMTI010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412117"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1227)
                     /locus_tag="VN039_12525"
     CDS             complement(196..1227)
                     /locus_tag="VN039_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV46821.1"
                     /translation="MNTHQQQCEAAIVAGAWDTLFAESMAWSRDPDGTKDPRPLFARN
                     VVYLLQGRFADAWKAHALCLEAEKDIAAVGTWVKDLLDRHTDSGYTNLVMGLFLAQSG
                     QSEQSMRPYSEAARLLPQSAYPHYFLAQVHERAGRLEMAIKGYREAVRLAPDYAPARM
                     NLGVAYQDQGRLEMAIKEYREVVKLIPTDSAAHSNLACALAEQGKMEPALQSYKTALK
                     LNPQDAEVHFALGGLYEMRGRLDLAHKSYQDALNADPDFGAAHSALGWLALGKHRLQE
                     ASDIFSRALKCNEEDARAYHGIAEIYALRGKRQSAMENYTKALKYYRDPEKKNQIMNQ
                     LFQQGQVGD"
     gene            complement(1243..2730)
                     /locus_tag="VN039_12530"
     CDS             complement(1243..2730)
                     /locus_tag="VN039_12530"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWV46822.1"
                     /translation="MSQDQSGRRVMAIAPMPPEKSAYALARYSRSPDSIEQSIRWVHG
                     HSSEKFWDQFYFDYGHASIADLGHVIICFEEISELAAIRLEDEPLWDGQAKSSRYQDF
                     ASDRWFVPGQIRGSETEAVYEGILRSLSEIYRLLHDPLIAHLSERDPRPESMKAADYQ
                     RTIAARAFDVTRYLLPLAAKTNVGQVVSIRTLEKQITRLLSSQLPELRAIGEDLKEAC
                     QRPPVNLWNELSGQTLNLNDPLAPTLARHAKANAYQESVYSDVARHAKEALRGTGLDQ
                     PGTWGAVEAVELIDPHHPLDEIVTTLLYRVSQAPYRKILEIVKEWSEKEKQETIEVAT
                     RQRGPYDELIKEFRSGYAFNFDILMDIGAWRDMHRHRRCQQVQQNFTTLHGYDVPSPL
                     IEAGLDQEYRQAMDAVRQDIELLKKKDQEASLYAIPFGFKVRCLFKMDYAEAEYISKL
                     RSGVKGHWSYRTVAWQMKQQLAKKFPFLGEAIHATPPDVEDALTR"
     gene            2895..>4053
                     /gene="hisS"
                     /locus_tag="VN039_12535"
     CDS             2895..>4053
                     /gene="hisS"
                     /locus_tag="VN039_12535"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWV46823.1"
                     /translation="MIKGIKGVKDLLPEETPRWRLIEETARRWAGRYGFHEIRIPIFE
                     VTTLFARSIGTSTDIVEKEMYTFQDRDGTSLTLRPEGTAGTVRAYIEHNRAAIPVPQK
                     YFYFGPMFRHERPQAGRLRQFHQFGVESLGMADPQADIEVIALLWRILSDLDLPGLTL
                     EINNLGYTADRDLYRPHLVSYLKQHESGLCANCRHRIDANPLRVLDCKVPDCRAITET
                     APRLSDSLSEAARSYFIRVLSGLDVVKIPYSLNHRLVRGLDYYNLTTFEVTATNLGAQ
                     NAVGAGGRYDGLVETLGGPSTPAVGFAIGIERMSILLPESTVRKTPEDVTVYVAGFGG
                     QGTVAGFTALEELRFAGFQAVSDFRSLTLKAHLRQADRLRCRFTLIIGDDEV"
BASE COUNT          823 a         1144 c         1109 g          977 t
ORIGIN      
        1 atgagataac ctgaaaattc aggctgcggt gctgagctgc cgcctgctgt ttgagtcgtt
       61 tcaccaatgg ttctggaatg ttcttgatgg tcaagctcgc catagattat cctcatggtt
      121 ccatattgca tccgttatgg acgctatacc gagcgtcaag atcccgtcaa gcgagaagag
      181 ttgacaggcg atccctcaat ccccgacctg tccttgctgg aacagctgat tcatgatctg
      241 attctttttc tcgggatcgc gatagtactt cagggctttg gtgtagtttt ccatcgcact
      301 ctggcgcttg ccccgcaggg catagatttc agcgatgccg tgataggccc gtgcgtcttc
      361 ttcattgcac ttgagcgccc ggctaaagat atccgaggct tcttgaagcc gatgtttgcc
      421 taaggcaagc cagccgagcg cagaatgggc ggcgccaaag tccggatcgg cgttgagtgc
      481 gtcttgataa ctcttgtgtg ccaggtccag gcgtccgcgc atttcgtaga gtccgcccag
      541 cgcgaagtgg acttcggcgt cctgtggatt cagtttcaag gccgtcttgt aggattgcaa
      601 cgccggttcc atcttcccct gttctgcgag tgcacaggcg agattggagt gggctgccga
      661 atcggtgggg atgagcttca ccacctctcg gtattctttg atcgccattt ccagccgccc
      721 ttggtcttga tatgcgaccc ccagattcat ccgtgccggc gcgtagtccg gtgcaagccg
      781 gacggcttca cgatacccct taatcgccat ttcaagacgg ccggcgcgtt cgtgaacctg
      841 tgccagaaag taatgcggat aggcagattg cgggagtaat cgagcggctt ctgagtatgg
      901 tctcatggat tgctccgatt gacccgattg cgccaggaaa aggcccataa ccaaattggt
      961 gtagccactg tccgtgtgcc gatcgagcag atccttcacc cacgttccca cagctgcgat
     1021 gtccttctca gcttcgaggc agagcgcatg ggctttccag gcatccgcaa accgaccctg
     1081 caagagatat acgacgtttc gcgcgaagag aggacgtgga tcttttgttc cgtccggatc
     1141 ccggctccac gccatcgact cggcgaacaa cgtgtcccat gccccggcca cgattgcggc
     1201 ttcgcattgt tgctgatggg tgttcatctg tgagacaacg tcttatctgg tcagcgcgtc
     1261 ttcgacatcg ggtggtgtcg cgtggattgc ttcgccgagg aacggaaatt tcttcgccag
     1321 ttgttgcttc atctgccaag cgaccgttcg gtacgaccaa tggcctttca cgcccgaccg
     1381 tagcttgctg atgtattccg cttccgcgta gtccatttta aacaaacacc gtactttgaa
     1441 gccgaacgga atcgcgtaga gtgaagcttc ctgatccttt ttcttgagca actcgatatc
     1501 ctgacgcact gcatccatcg cctgacgata ctcctgatcc aggcctgcct caataagcgg
     1561 agacgggaca tcatagccgt gaagagtcgt gaaattttgc tgcacttgct gacaccgtcg
     1621 atggcggtgc atgtctcgcc aggcgccgat gtccatgagg atatcgaagt tgaatgcata
     1681 gccgctgcgg aactctttga tgagttcatc gtatggcccg cgctgtcttg tggccacttc
     1741 gatggtctct tgcttctcct tctcagacca ctctttcacg atctcgagta tcttccggta
     1801 cggggcctgc gacactcggt aaaggagtgt cgtgacgatt tcgtcgagcg gatgatgggg
     1861 gtcgatcagc tcgaccgcct ccaccgcacc ccatgtacca ggctgatcta atcctgtgcc
     1921 gcgcaatgct tccttcgcat ggcgagccac atcggagtag accgattcct gataggcgtt
     1981 cgccttggca tgccgtgcca gtgtcggcgc cagaggatcg ttcaagttta aggtctgacc
     2041 gctgagttcg ttccacagat taaccggcgg tcgctggcag gcctctttca aatcctcgcc
     2101 gatcgcccgc agctcaggca attgtgacga caacaggcgc gtaatctgtt tttccaacgt
     2161 ccggatactc acgacttgcc cgacgttggt cttggccgca agaggaagca ggtagcgggt
     2221 gacatcaaag gcgcgtgccg cgatcgtccg ctgataatcg gccgctttca tagactccgg
     2281 ccgtgggtcc cgttcagaga ggtgcgcgat caagggatcg tgcagtaatc gatagatttc
     2341 ggacagactg cggagaatgc cttcatagac cgcttcggtt tcgctccctc gaatctgacc
     2401 cggcacaaac catcggtcgg aggcaaagtc ctgatagcga ctggatttcg cttgaccatc
     2461 ccaaagaggc tcatcctcca agcgaatagc ggcgagttct gagatctctt caaaacagat
     2521 gatgacgtga ccgaggtcgg cgatcgatgc atgaccgtaa tcgaaataaa actggtccca
     2581 gaacttctcc gaggaatgtc catgcaccca ccggatgctt tgctcgatgg agtccggcga
     2641 tcggctgtag cgcgctagtg cgtatgcgga tttctccggc ggcatgggag cgatggccat
     2701 gacccggcga cctgattgat cctgactcat agaaggcctt tcacgctgta ctgaaccata
     2761 ggccgcgcac tatacctctc acgtcagtat ggcgcaagct gccgatccgt tgacttgccg
     2821 cagaagccgc cgctatagtc cgcgcgaact gtgccgcatg atacgcacct tcgtacagag
     2881 atgatcgggt acccatgatc aaggggatta aaggtgtcaa agacctgttg ccggaagaga
     2941 caccgcgttg gcgcctcatc gaagagactg ccaggcggtg ggccggccgc tacggatttc
     3001 atgaaattcg cattccgatc tttgaagtaa cgaccttgtt tgcgcggagc atcggtacat
     3061 cgaccgacat tgttgagaag gaaatgtaca cgttccaaga tcgggacggt acctcgctta
     3121 ctcttcgacc tgaagggacc gccgggacgg ttcgcgctta tatcgagcac aatcgcgccg
     3181 caatccccgt tcctcagaag tatttctatt tcgggcccat gtttcgccac gagaggccgc
     3241 aagcaggccg cctgcggcaa tttcatcagt tcggcgtcga atcgcttggt atggctgatc
     3301 cccaagccga catagaggtc atcgccctcc tctggagaat cctgtccgat ctcgatctac
     3361 ccggcctcac cttggagatt aacaacctag gctatactgc tgatcgagac ctctatcggc
     3421 cccacctcgt gagttacctc aagcagcacg aatccggcct ttgtgcgaat tgccgacatc
     3481 gcatcgatgc caatcctctg cgggtcctgg actgcaaagt ccccgattgt cgcgcaatta
     3541 cggaaaccgc tccccgctta agcgactcac tgtccgaggc agctcgttcg tacttcatac
     3601 gagtcttatc gggtcttgat gtcgtgaaga ttccgtactc gttgaatcat cggcttgttc
     3661 gaggtcttga ctactataac ctcaccacct tcgaggttac tgcgaccaat ttgggtgccc
     3721 agaatgccgt aggggccggt ggacgctacg acggattggt tgaaactctc ggagggccgt
     3781 ccacaccggc tgttggtttt gcgatcggca ttgaaagaat gtcgatcctg ctgccggaat
     3841 ccacggtaag aaaaactccg gaggatgtga ctgtctatgt cgccgggttc ggtggtcagg
     3901 gtaccgtggc tggtttcacg gctcttgaag aacttcgttt tgcgggattc caggcagtct
     3961 ccgattttcg gtcgctaacc ctgaaggctc acttacggca agctgatcgc ctcaggtgtc
     4021 gattcactct cattatcggg gatgacgaag tag
//
LOCUS       DATMTI010000791         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_243237, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000791 DATMTI010000000
VERSION     DATMTI010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243237"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VN039_12540"
     CDS             <1..790
                     /locus_tag="VN039_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46824.1"
                     /translation="AKVLKSFFNQSLGEPYTSPGDQVTKELLDACIDPAHQLGGIPNG
                     AVYLGIDVGHDEIYVEANYRTRHGALALWNLWHIRDKPGKTAWEILDEKVLSKLSAWL
                     CVCDAHPDKRGARYLSVKYPGKFWMGFEKDRPEQEELAKWDKVTYREPNKVIIDRTLA
                     FDTVINDMGTGNCILPHGARELGEQMPRRDYNAYYSHMIQMVRVEEPDARGRIVASWV
                     KNKNPDHWHHAKMFSWIASTRRAPMGVSPAAAKALRNAGTPVGV"
     gene            792..1403
                     /locus_tag="VN039_12545"
     CDS             792..1403
                     /locus_tag="VN039_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46825.1"
                     /translation="MPKNKKTPAERAEILEKAHSKAKKGYRINTKRDRKDVGDDEMAV
                     IKDMLVSLRLVGYTPSQCAAVVGLSKRQVKEIVNDPNFKSRLESLKTKLPEAAVNLGR
                     AYLVEAVQAVVHILRTETDNALILKAAAEIFDRFGIPKVSRAEVKSDPTPEQSGSEIN
                     ETFMDRLRTAPPEIQEKVAALHESFTEGVERILSEGKPDGDTD"
     gene            1387..>2753
                     /locus_tag="VN039_12550"
     CDS             1387..>2753
                     /locus_tag="VN039_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46826.1"
                     /translation="MGILTKRDQTSLDRPPLARPKKSRIGEITTSWARYFAGGIRRTG
                     LWPTPPQYQSGATALTYALARAIYRNDNKGSNLGAGFGRRVINSRVDFIDLPMSATGD
                     EIIDEFLNNAIHTYWAGVLLQAFRDACRDAETIVRIRRHDVNNKLVTADEWESCYLEI
                     VPPETCAIYYKQQGDTTEIEAAYVRHEIDVKFEDATVNGSYLSQPVVRTQVVIEELTP
                     ESFRYFNETEGRWMDEIEETNSWGFVPLVEGYNEYDAVLKGGQSDFESCLPFIMAFHD
                     VVAQTLVAHKAHSIPKAKFKVHDMMNFIANNWPESFDLDETGRPALDSFNGNLSWKGT
                     EMLFFDAEGEDAEFLEVQSALGDSKTLLDFLLTCIAISSETPKSLLMDQTVQDADEMT
                     PFSKMINRKRRHFANMIQTICKMVLAINLMEPTRVPLSWEEITPDIALKKSQALQQDV
                     MSYETL"
BASE COUNT          642 a          792 c          787 g          532 t
ORIGIN      
        1 cgccaaggtg ttgaaatcgt ttttcaatca gtccctcggc gagccgtaca cgtcaccggg
       61 agaccaggtg acgaaggagt tgctcgatgc gtgcatcgat cctgcacatc agcttggcgg
      121 aattccgaac ggggctgtct atctggggat tgacgttggg cacgatgaaa tctacgtcga
      181 agccaactat cggacgcgac acggtgctct cgcgctctgg aatctctggc acatccgaga
      241 caagcctgga aagaccgcct gggaaatcct cgatgaaaaa gtcctgagca agctcagcgc
      301 atggctgtgt gtctgcgatg cacacccgga taagcggggt gctcgctatc tgtcggtgaa
      361 ataccccggc aagttctgga tggggttcga gaaggatcgc cccgaacagg aagagcttgc
      421 gaagtgggac aaggtcacgt accgcgaacc gaacaaggtc atcatcgacc gcacgcttgc
      481 attcgacacc gtcatcaacg acatgggcac cggcaattgc atcctgccgc acggtgctcg
      541 cgaactcggt gagcagatgc ctcgccgtga ttacaacgcc tactactcgc acatgatcca
      601 gatggttcgt gtcgaggaac ctgacgctcg cggtcgcatc gtcgcatcgt gggtgaagaa
      661 caagaaccct gaccactggc accacgcgaa gatgttttca tggattgcat cgacgcggcg
      721 tgccccgatg ggcgtatccc ctgctgctgc gaaggcgctt cgcaatgcgg gtacgccggt
      781 aggagtctga aatgcccaaa aacaagaaga ctcccgcaga gcgcgctgaa atcctcgaga
      841 aggcacacag caaagccaag aagggctacc gcatcaacac caagcgcgac cggaaggacg
      901 ttggtgacga tgaaatggcg gtcatcaagg acatgctcgt ttcacttagg ctcgtcggtt
      961 acacgccgtc gcagtgcgcg gcagtcgtgg gcctgtcaaa gaggcaagtc aaggaaattg
     1021 tcaacgaccc caacttcaag tcccggctcg aatcgctgaa aacgaaactg ccggaagccg
     1081 ctgtcaacct ggggcgtgcg tatctcgtgg aggcggttca ggctgtcgtc cacatcctgc
     1141 gcacggagac cgacaatgcc ctcatcctga aggctgcggc tgaaatcttc gaccgcttcg
     1201 gcatcccgaa ggtgtctcgg gccgaagtca aatctgaccc gacacccgaa cagtccggaa
     1261 gtgaaatcaa cgagacgttc atggatcggc tgcgtaccgc gcctcctgaa atccaggaaa
     1321 aggtggcggc tctacacgag agcttcactg aaggtgtcga gcgcatcctg agcgaaggga
     1381 agcccgatgg ggatactgac taagcgcgat cagacttcac ttgatcgccc accgcttgcg
     1441 aggccgaaga agtctcgcat cggtgaaatc acgactagtt gggcacgtta tttcgccgga
     1501 ggaattcgcc ggacgggcct gtggcccaca ccgccgcagt accagagcgg tgccactgct
     1561 cttacctacg cgctcgcgcg tgccatctac cgcaacgaca acaagggttc caacctcggc
     1621 gctggcttcg gtcggcgcgt catcaattcc cgtgtcgatt tcatcgatct tccgatgtcc
     1681 gccacgggtg atgaaatcat cgatgagttc ctcaacaacg caattcacac gtattgggct
     1741 ggtgttcttc ttcaggcgtt tcgtgatgcc tgtcgtgacg ccgagaccat cgtgcgtatt
     1801 cgtcggcacg atgtaaacaa caagctcgtc accgccgacg aatgggaatc gtgctacctc
     1861 gaaatcgtcc cgcccgagac ttgcgctatc tactacaagc agcagggcga cacgactgaa
     1921 atcgaggcgg cgtacgtccg ccatgaaatc gatgtgaagt tcgaagacgc gactgtcaac
     1981 ggttcgtacc tgagccagcc ggtcgtgaga actcaggtcg tcatcgaaga actgacgccg
     2041 gaatcgttcc gttacttcaa cgagaccgaa ggccggtgga tggatgaaat cgaagagacg
     2101 aactcctggg gcttcgtccc gctcgtggaa ggctacaacg agtacgacgc ggtgctgaag
     2161 ggtgggcagt ccgatttcga atcgtgcctg ccgttcatca tggcatttca cgatgtcgtc
     2221 gcgcagacgc tcgtggcgca caaggcccac tcgatcccga aggccaagtt caaggtgcat
     2281 gacatgatga atttcatcgc gaacaactgg cccgagtcct tcgacctcga tgaaaccggc
     2341 cgtcctgccc tcgattcgtt caacgggaac ctttcgtgga aggggactga aatgctcttc
     2401 ttcgacgcgg aaggtgagga cgctgaattc ctcgaagtcc agtccgcact cggagactcg
     2461 aaaacgcttc tcgacttcct gctcacgtgc attgcgattt catctgaaac gcccaagagc
     2521 ctgctgatgg atcagacggt tcaggacgcg gatgaaatga caccgttctc gaagatgatc
     2581 aaccgcaagc ggcggcactt cgccaacatg atccagacca tctgcaagat ggttctcgcg
     2641 atcaacctta tggagccgac acgggttccg ctgtcgtggg aggaaatcac gcccgacatc
     2701 gccctgaaga agtcgcaggc acttcagcag gacgtgatga gctacgaaac gct
//
LOCUS       DATMTI010000792         3952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_666165, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000792 DATMTI010000000
VERSION     DATMTI010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666165"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VN039_12555"
     CDS             complement(<1..308)
                     /locus_tag="VN039_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46827.1"
                     /translation="MSTRQRFFSYGLTLALFGAALIPMTVLAEDRSFYSPVIYIDQEQ
                     NQILISTSGKVFYVEVPEAAKPHIEKLPISGLVDFVVEMRGEDQRPLIKTWKVKSGES
                     T"
     gene            complement(384..893)
                     /locus_tag="VN039_12560"
     CDS             complement(384..893)
                     /locus_tag="VN039_12560"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWV46828.1"
                     /translation="MADLIIPRQILDDIIAHAKELTPYECCGLLAGTNGVVSRLYRIK
                     NIVAMEGAQNLSSFDSAKAAHLERLSPAERAEIAFVMDMQDFSASKKDMRNNGLDLQV
                     IYHSHPHDPARPSITDIKIATDYEEIWPKINLPLPAYLLVSLMNAEPDVKTYWIKSGR
                     VTPTDILIR"
     gene            complement(1099..1902)
                     /gene="moeB"
                     /locus_tag="VN039_12565"
     CDS             complement(1099..1902)
                     /gene="moeB"
                     /locus_tag="VN039_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWV46829.1"
                     /translation="MEFTEQQIQRYSRHIILQDVGGKGQLKLNKAKILLIGAGGLGSP
                     AGLYLAAAGIGTIGLVDGDVVDLSNLQRQIMHSTATLGQPKVESGRKTLSAINPDIAI
                     KTYHQLVDAENILPLVSQYDIVLDGSDNFTTRFLVNDACFFAKKTLISASMFRFEGQL
                     TAIKPHQGYPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGE
                     TIADKLLIYDALEMKFRKVGRPKDPACPLCGLNPKIKDLSLDYTVTCTI"
     gene            complement(1999..2238)
                     /locus_tag="VN039_12570"
     CDS             complement(1999..2238)
                     /locus_tag="VN039_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HWV46830.1"
                     /translation="MAHLRFHIRFPEDKIREPIIYQIGREYNVVTDVRRADVRDTTGW
                     ADVELSGDTVEIERAVAGLRAKGCVVDPIELNVVE"
     gene            complement(2241..2513)
                     /locus_tag="VN039_12575"
     CDS             complement(2241..2513)
                     /locus_tag="VN039_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWV46831.1"
                     /translation="MIKVRIPTPLRPLTKGQGEVEANAGSVVEMIEALNSTHPGIKDR
                     LCDETGELRRFVNIYVNEEDIRFLKGKDTSLKDGDEVSIVPAIAGG"
     gene            complement(2510..3757)
                     /gene="thrC"
                     /locus_tag="VN039_12580"
     CDS             complement(2510..3757)
                     /gene="thrC"
                     /locus_tag="VN039_12580"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWV46832.1"
                     /translation="MSKMKALVCRECGKEYPTKAIHVCEMCFGPLEVKYNYDEIRKTI
                     SRKKIEDGPRSMWRYLDLLPVEGTNFVGPHAGFTPLVRAKNLGAYLGLDELYIKNDTV
                     NHPTLSFKDRVVAVALTRARELGFETVACASTGNLANSVAAHAASASLHCYVFIPGDL
                     EAAKVLGNLIYKPHVVEIEGNYDDVNRLCSEIAGEHGWAFVNINIRPYYAEGSKTLAF
                     ETVEQLGWKTPDQVVIPMASGSLLTKIWKGLHEMKALGLIDTVHTKINGAQAEGCSPI
                     STAFKAGRDFFKPVKPQTIAKSLAIGNPADGYYALKATAESHGSMDMVTDEEVVEGIQ
                     LLAQTEGIFAETAGGVTIGVLKKLVKQGVIKKDEVTVAYVTGNGLKTQEAVIDSVGRP
                     VRIQPSLVDFEKTFKMGKNGGDA"
BASE COUNT          842 a         1046 c         1096 g          968 t
ORIGIN      
        1 gtcgattcgc ctgactttac cttccaggtt ttaatcagcg gacgttgatc ctctccccgc
       61 atttcaacga cgaaatccac aaggccggaa atgggaagct tttcaatatg tggttttgcg
      121 gcttccggca cttcaacata aaagaccttc ccgctcgtgg agatgagaat ctgattctgc
      181 tcctgatcga tgtaaattac cggactgtaa aaactgcgat cctcggcgag gaccgtcatt
      241 ggtatgagag cggccccgaa cagtgcgagg gtgaggccat agctaaagaa tctttgtcgc
      301 gtggacatgc acaacgctcc ttattcaggc tgcacgggaa cattattaca ttccggcgaa
      361 gatgtgaact gtaaaaatct gtctcaacgg atgagtatgt cggtgggagt gacgcgaccg
      421 gatttgatcc aataggtctt gacgtccggt tctgcattca tgagcgaaac caggagatag
      481 gcggggaggg gcaagttgat cttgggccaa atctcttcgt agtcggtggc aatttttatg
      541 tcggtgatcg acggacgggc cgggtcgtgg gggtgcgagt gatagatgac ttgtagatcg
      601 agtccattgt tgcgcatgtc cttcttcgag gctgagaaat cctgcatatc catgacaaaa
      661 gcaatttccg ctcgttcggc aggcgaaagc cgctcgagat gcgcggcttt tgcggaatcg
      721 aatgaagaga ggttttgtgc cccctccatc gcaacgatat tcttgatgcg atagaggcgg
      781 ctgaccactc cattagttcc ggcgagcaac ccgcaacatt cgtaaggagt aagttccttg
      841 gcatgggcga tgatgtcgtc gagaatctgt cgggggatga tgaggtcggc cacgatcagt
      901 cgatcctgaa gtagccgaaa cgattttgag tcagtggtgg aggggattgg tggaatattg
      961 gttgatgtgt gtgcaggatg gtcaaaacgg ctgtccagca aggccgcagc gagcgaaggg
     1021 ccgaggcgta cagtttgagg tacgttgagg gcctgagcga tgcgagaacg aagctggcgg
     1081 gcgttttcaa catcctgttt aaatcgtgca ggtgacggtg tagtccagac taagatcttt
     1141 gattttggga tttagtccgc agagcggaca ggccggatcc ttggggcgac cgacttttcg
     1201 gaacttcatc tcgagcgcat cgtagatcaa caatttatca gcgatcgtct ctccgatgcc
     1261 gaggatttct ttgatcgctt ccgacgcctg tagaattccc attgtgccgg cgaggacacc
     1321 gagcacaccg gcttcttgac aattcggaac cagcccagcc ggcgggggct cgggatagag
     1381 acaacgatag caaggatagc cttggtgcgg cttgatcgcc gtcagttgcc cctcgaaccg
     1441 aaacatgctg gcggagatca atgttttctt ggcaaagaaa caggcgtcgt tgaccagaaa
     1501 ccgcgtcgtg aagttgtccg atccgtccag cacgatatcg tattgcgaga ccagaggaag
     1561 gatgttttct gcatcgacca actgatgata ggttttgatg gcaatgtcag gattaatggc
     1621 cgacaaggtt tttcttccgg actcgacttt tggctgcccg agcgtcgcgg tcgaatgcat
     1681 gatctgtcgt tgtagatttg agaggtcaac gacatcccca tcgaccaggc cgattgtccc
     1741 aatgccggcg gcagcgagat acagaccggc cggcgagccc aggccgcccg caccgatcag
     1801 caaaatcttg gccttgttca acttcagctg accttttcct ccgacatctt gaaggatgat
     1861 atgccggctg taacgttgga tttgttgttc ggtgaattcc attgtctgtc ccgtaaggcg
     1921 tgaaatgtta ggcgttaggc gacgtgattc tcagctggtt ttgtactcct tacgcctcac
     1981 cccttacccc taacggaatc attccaccac gttcaattca atcggatcga cgacacagcc
     2041 tttggctcgc aacccagcga cggcccgttc aatttccacc gtgtccccgg aaagctccac
     2101 atccgcccaa cctgtggtgt cgcgaacatc ggctcgtcgg acatcggtca caacgttgta
     2161 ctcgcgcccg atctgataga taatcggctc tctaatcttg tcctcgggaa aacgaatatg
     2221 gaagcgtaag tgtgccatga ttatcctccc gcgatcgccg ggacgatgga aacctcatcg
     2281 ccatccttga gggaggtatc tttccctttc agaaagcgga tgtcttcttc gttgacataa
     2341 atgttcacga agcgacggag ttctcccgtt tcatcgcaca ggcgatcttt gatgccggga
     2401 tgtgtgctat tcaatgcctc gatcatctcc accacactgc cggcattggc ctcgacctcg
     2461 ccctggcctt tggtcagcgg gcgaagcgga gtcggaatac gaactttaat catgcgtcac
     2521 caccgttctt tcccatttta aacgtctttt cgaagtccac caggctgggc tgaatgcgta
     2581 ccgggcgacc gacggaatcg atcaccgctt cctgtgtttt caatccattg ccggtgacat
     2641 aggctaccgt cacctcatct tttttgatca cgccttgctt gacgagcttc ttgagtacgc
     2701 cgatcgtgac gcctccggct gtttccgcga agataccttc ggtttgggcc aataattgga
     2761 tgccttcaac cacttcttca tccgtcacca tgtccatgga tccatggctc tcggcggtcg
     2821 ccttgagcgc gtagtaccca tcggcagggt tgccgatggc aagggacttg gcgatcgtct
     2881 ggggtttcac cggtttgaag aagtcgcgtc ccgccttgaa tgcggttgag atcggtgagc
     2941 agccttcggc ttgggcaccg ttgatctttg tgtgaacggt gtcgatgagg cccaaggcct
     3001 tcatctcatg cagccccttc caaatcttgg tcaaaagaga acctgacgcc atcggaatga
     3061 cgacttgatc cggcgttttc cagcccagtt gttccacggt ctcaaaggcg agggttttgg
     3121 agccttcggc atagtacgga cgaatgttga tattcacgaa cgcccatcca tgttcgccgg
     3181 cgatctcact gcacaaccga ttgacatcgt catagttgcc ttctatctcg acgacgtgcg
     3241 gcttatagat cagattgccg agtaccttcg cggcctcaag atcgccgggg atgaatacat
     3301 aacagtgaag actggcggat gcggcatggg cggcgactga gttcgccaag ttgccggtcg
     3361 atgcgcaagc tactgtttcg aaaccgagct cgcgcgcgcg cgtgagggcc acggcgacga
     3421 cgcgatcttt aaacgacagg gtcggatggt tcaccgtgtc gttcttaata taaagctcgt
     3481 cgagtccaag ataggcgccg agattttttg cgcgtacgag cggggtaaat cccgcgtgcg
     3541 gtcccacgaa gttcgtccct tcgaccggca gcaagtcgag gtagcgccac atgctgcgcg
     3601 gaccatcctc gatcttctta cgcgaaatag tcttcctgat ctcgtcgtag ttgtacttca
     3661 cctcaagagg accgaagcac atttcgcaga catggatcgc ctttgtcggg tattccttgc
     3721 cgcactcgcg gcaaaccagc gctttcattt tgctcatcgt cgcaccacct tatcaaaccc
     3781 tatgcagagg gacgcatggc gcatccggca taggggtccc cgtcgtcgaa cagttcggta
     3841 atcctcggat gttccccgca gagcgcacag ttcgggttcc ggcggacttt aatttctcgg
     3901 aattgggttt tgcgcgcatc gaagtcgagc aggcggtcgg tcagctgccg tc
//
LOCUS       DATMTI010000793         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_243273, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000793 DATMTI010000000
VERSION     DATMTI010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243273"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VN039_12585"
     CDS             complement(<1..830)
                     /locus_tag="VN039_12585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV46833.1"
                     /translation="MDWLSQQDVPKDQYELIWVEVYDRVIDEVMEKADVVITCNQQGV
                     YHKHIGYNVGLLHARGELICVCDSDAVFPPDFIRSVFRSFQMADGGSAVPLVLMHYEW
                     RTSLLYPDGLTSTETLKDHTRWQWWPLIVNEGACMTVRRADAIRFGGFDEHPSYRGYL
                     CGPYDLGWRLINAGWPELWHDPSVALWHFAHPDPIGTNGQQASLKQLLEKAYPHIDGH
                     ALTAVEAFSTGRFQPLRENPDIWALRMRDRRIGSELETRYADLTGQGGYSPWVVRSLW
                     V"
     gene            complement(1016..>1677)
                     /locus_tag="VN039_12590"
     CDS             complement(1016..>1677)
                     /locus_tag="VN039_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46834.1"
                     /translation="QDYGSRLYDEASATFSKGRIGRHMECFEERHFALFDALPQDFME
                     ALGYSRSSLTSSRTKELRHRPLVVKSLSQDALYTPRLVREGVLGWNIVEIAGKYFPVR
                     HGVRIISADDAKVVSEEQEGFWSLRDAMDALIHGAASTARPAAPEITGPVLTVEGYCG
                     FNIVRYNGGWYGFNQSSGDIDIRSLSESALKVMKGNKHCVTGENVADVKAEILRLYMN
                     R"
BASE COUNT          415 a          481 c          407 g          374 t
ORIGIN      
        1 acccataacg atcgcacaac ccaaggtgaa taaccgcctt gtccggtcaa gtcggcatac
       61 cgcgtttcga gttcggatcc gatgcgccga tcccgcatcc gcaaagccca gatgtcagga
      121 ttttcacgca gaggttgaaa gcgacctgta gaaaatgctt ccacggccgt gagtgcatga
      181 ccatcaatgt gaggataggc cttttccagc agctgcttca gcgaagcctg ctgaccattc
      241 gtgccgatgg gatccggatg cgcaaaatgc cagagggcca ctgacgggtc atgccacagc
      301 tcgggccatc cggcgttgat aaggcgccat cccaaatcgt acggcccaca cagatagcct
      361 ctgtaactgg gatgttcatc aaaccctccg aatcgtatcg catcagctcg acgcacggtc
      421 atgcaggcgc cttcgttgac aatgagaggc caccactgcc agcgggtatg gtctttgagt
      481 gtctcggtgc tggtcaggcc gtccggataa agtagcgacg tgcgccattc atagtgcatc
      541 agtaccagtg gaacagccga cccaccatcc gccatctgaa aggagcggaa cactgaacga
      601 ataaaatcgg gaggaaatac tgcatcgctg tcacacacgc agatcagttc accgcgcgca
      661 tgcaacaatc ccacattgta gccaatgtgc ttgtgataga caccttgctg attacatgtg
      721 atgacgacat ccgctttttc catcacttcg tcgatgacac ggtcatagac ttccacccag
      781 atcagttcat actgatcctt gggcacatcc tgttgcgaca gccaatccaa ggtagtgaac
      841 cgctcgcgac aaccccagtc caatagaaca atggataaac ggggtgaaga tggcgatgcg
      901 tttttgtgaa caatggctct catcgtctgc attctttccg tttgagagga cagcatgccg
      961 gacgaacaat cctgtcaacc gaatttcata acattgtaga gtacgacccg ttgtttcatc
     1021 tgttcatgta gagacggagg atctccgcct tgacatcggc gacgttctcc cctgttacac
     1081 agtgcttgtt ccctttcatg accttcagag cggactcgct gagactcctg atatcaatgt
     1141 cgccacttga ctgattgaaa ccataccaac cgccgttgta acgaacaatg ttgaatccgc
     1201 aatagccttc gaccgttaat actggtcctg tgatctccgg tgctgctggt cgagccgtac
     1261 tcgctgcacc atgaatcaga gcatccatgg cgtcgcgcaa gctccagaaa ccctcttgtt
     1321 cttccgagac aaccttggcg tcatccgcag atatgatgcg cacaccatgt cgaactggga
     1381 aatacttccc tgcgatctca acgatgttcc atcccaatac accttcccgc accaaccgcg
     1441 gcgtgtagag cgcatcctga gataaacttt tcacgactaa gggacgatgc cgtaattcct
     1501 tcgttctcga agaagtcagc gagctgcggg agtatccgag ggcttccata aaatcttgtg
     1561 gcaaggcatc aaaaagcgcg aaatggcgct cctcgaaaca ttccatgtga cgaccaatcc
     1621 gtcccttgga aaaggtggca ctggcctcgt cgtaaagacg ggaaccatag tcctgcg
//
LOCUS       DATMTI010000794         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_837347, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000794 DATMTI010000000
VERSION     DATMTI010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837347"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="VN039_12595"
     CDS             <1..1566
                     /locus_tag="VN039_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46835.1"
                     /translation="VFLRRARVRLDWGRWIGIKPCGLVNETRLNLASRSNWQGITPND
                     LRAMAAGALRVPIEEVRWFYRDEDLAIDPKGMATIRQRKDTLYVLDGGGFETMRFMAC
                     MGAMHWDQIDFLPVVELFKSLLPGTGSAVFELIRGLYDDQAKGHSAPRALRYRGIPTY
                     PSEAAFRLFSGFFTPQPLDGEDPFTAFMNPSKSHLVVWLPAQYPPVRYFDESQGLCLT
                     VKNGIAQKATLAEDPAGLPYVSSIGRRVLPLDRSLHVEGSLLILRDRETELTLPLNHD
                     LMVRPSPSGECPISPVDWRAVFVPGIPRISPAEAFEAVPLFPEDDTEISELAAQSFVA
                     DYLDDLGEQNREVGRLRSQAERVFIANGDAVISTCILFDRPRDYTVNVRYVAYAQRQA
                     QQLWTQCAEVQRWDWLQRIRMVAEDAREESPTNCGPYDLIYQWLPYDSFDSSTTMRTI
                     VTRLSQMLRRGGEAFVVGPAQLGELLTQERSRLLVHWDQSVASLPTVLMHKTILPKAR
                     VKLGLTLFRVGRL"
BASE COUNT          324 a          406 c          477 g          402 t
ORIGIN      
        1 gtctttctgc ggcgcgcgcg agtccgactt gattgggggc gatggatcgg gattaagccc
       61 tgtgggttgg tgaatgaaac caggctgaat ctagcatcac ggtccaattg gcaagggatc
      121 actccgaatg atcttcgtgc catggctgcc ggggcgttac gagtacccat cgaggaggtg
      181 cggtggtttt atcgcgatga ggatcttgct attgatccca aagggatggc cacgattcga
      241 cagcgaaaag acacattgta tgttttggat ggtgggggat ttgaaacgat gcgcttcatg
      301 gcctgcatgg gagccatgca ctgggaccag attgactttc tcccggtggt cgaactgttc
      361 aaatcgttat tgcccgggac gggctctgcc gtgttcgagc tgattcgcgg attgtatgat
      421 gatcaagcca agggacactc tgctccgagg gcgttacggt atcgtgggat ccctacctat
      481 ccatccgagg cagcctttcg cttgttcagc ggatttttca cacctcagcc gttagatggt
      541 gaggacccat tcacagcttt catgaatccc tcaaaatctc atcttgtcgt ctggctgccg
      601 gcccaatatc cacccgttcg gtattttgac gagagccaag ggttgtgtct gaccgtgaaa
      661 aacgggattg ctcaaaaggc cacgcttgcc gaagatccgg ccggtcttcc atacgtcagt
      721 tcgattggcc gtcgtgtact cccgctggat cgtagcctcc atgtcgaagg aagtctgttg
      781 attttgagag atcgtgaaac ggaattgacc ttgccgttga atcacgatct catggtgagg
      841 ccgtcgccgt ctggcgaatg tccgatcagt ccggtcgatt ggcgcgcggt attcgtgcca
      901 ggaattccga ggatttcgcc tgccgaggcc ttcgaggcgg tgcccttgtt tcccgaagac
      961 gacacggaga tcagcgaact cgccgcccaa tccttcgtcg cggactatct cgacgatctt
     1021 ggggaacaga atcgagaggt tggacggcta cgatcgcagg cagagcgtgt gttcattgcg
     1081 aacggggatg ccgtcatttc cacttgtatt ctctttgacc ggccacgtga ctataccgtg
     1141 aatgtccgct acgttgccta tgcacagcgt caggctcagc agctctggac tcaatgcgct
     1201 gaggtgcaac gatgggattg gctgcagcga atccgaatgg tagccgagga tgcgcgtgaa
     1261 gagtccccga caaactgcgg accttatgac ctgatttatc agtggctgcc atatgactcg
     1321 tttgattctt cgacgaccat gagaacgatc gttacgcgat tgagtcaaat gttacggcgc
     1381 ggtggtgaag cctttgtcgt cggcccagct caactagggg agctgttgac gcaggaacgg
     1441 tctcggttgc tggtgcattg ggatcaatca gttgcatctc tcccgaccgt tctcatgcat
     1501 aagacgatct tgccgaaggc cagagtcaag ctaggtctta cgttgtttcg tgtcgggcga
     1561 ttgtaggcca gcaggcagaa cttgggttgg ggccgtagcc tttcttcag
//
LOCUS       DATMTI010000795         4330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_243314, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000795 DATMTI010000000
VERSION     DATMTI010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243314"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..2860)
                     /locus_tag="VN039_12600"
     CDS             complement(203..2860)
                     /locus_tag="VN039_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWV46836.1"
                     /translation="MFNMAWRETRAAWRHFLYFLVCIAIGVGALTGVSLFGTQVERAV
                     TKEARGLLGGDLEIRLSRLMSPKGREVLDSLSDRGVALTHVSELVAMASRTGSSGSGQ
                     STQIIELKAVEPQYPLYGTLRLEAQGGLVGLLDRQARRCPDRTCFGVVVQESLLIRMG
                     LAVGDPLKIGQGLFIITGIVKTEPDRMANAFSLGPRVLMSREGLLTAELVKMGSRIRE
                     RYLLKIPSTMAPEPLLYELRGQLAPDSARVSSYRDAQPQLKQSLEQLTRYLGLIGLTA
                     LFVGGLGVATSVHAFVREKLNTIAILKTVGAESSTIIRTYGLQAMILGLVGSLVGLML
                     GVLFHQGLPLMIATLTASDLLDQLGFTMNGMELALAPLAKGLALGMLSTLLFALWPLL
                     TIRDVKPAWIFRREIAPLTATTPHPTAAWWEAWRKLDRVKILVSVAIGLGLALLSVWQ
                     AGSWKVGLLFVLAFAAAVLLLGTSARGVLMALKKWPCPGPLVLRQALGNVVRPGSQAV
                     SITIAIGIAVMVVTTVSLVERSLLTQVGDNRPTDAPTFFFIDIQPDQAEGMAALLRRQ
                     SSDPHPRLTPLVRSRLSAVKDEPVKFEATSEEEEQKEKAAQKEERRKKWYVTREYVLT
                     FLQDLPKDNKIVQGEWWKPGQDFTKPLISIEEDAAKQLGLTVGDTVELDIQGVPITGE
                     ISSIRQVEWGNFSTNFYMIFSPGALDGAPHTFVATVHVAPSEEVALQQAVVASFPNVT
                     AINMSDVLDSFARVLDRLSLAIRAVALFCVLSGGLVMAAALAATRYRRLYESVILKAL
                     GATRGVIVRSFAVEYALLGALGGLLGCALASALSWAVLSTVFDLSWRLQPAVLVMGFT
                     ATISLTMLVGFLSTYRLLGQPPLAVLRHE"
     gene            complement(2869..3645)
                     /locus_tag="VN039_12605"
     CDS             complement(2869..3645)
                     /locus_tag="VN039_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV46837.1"
                     /translation="MITVKDVSMALAAASRSVTILDRITFDIPAKQTVAIVGPSGSGK
                     STLLGLMAGLDRPTTGSIQLDGTDITTMGESQMARFRREKVGYIFQSFHLIPTLTAIE
                     NVAVPLELSGVTRTGDRAAELLVAVGLSDRMGHYPVQLSGGEQQRVAVARAFACRPPI
                     LLADEPTGNLDSSTGSHVIELLLSLHRDYGTTLVLVTHDAVLASSMQRVLSLHDGHLE
                     SDSIPASSKFLGDFLAASPSGKRAPARNPSSSDSFPIFGS"
     gene            3728..>4330
                     /locus_tag="VN039_12610"
     CDS             3728..>4330
                     /locus_tag="VN039_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWV46838.1"
                     /translation="MKRFHDILVPTFLMLLWPLTSGAISSSASDNRPRIVAFGDSLTA
                     GFGVQADESYPAQLQRRLDSLGYHYRMINAGVSGDTTAGGLRRVPWILNSKPELVILE
                     LGANDGLRGLPVDQTKSNLRQIIRQLQEAGTTVVLAGMKLPPNYGQDYTTNFEAMYQM
                     LAKEYHLALIPFFLEGVGGSSSLNQADGIHPTKEGYEIIVN"
BASE COUNT          986 a         1241 c         1215 g          888 t
ORIGIN      
        1 tattccgagg ctgccttccc gattacgatt gatgaggccg tcgtgcgatc gtcaactgat
       61 ggggcgatcg gcctgcgttt cactcaactc cgccgaagcg atgagcggcg cattcgccgg
      121 ctcatcgatg tgtggctgcc cgaactcttg ccgtcaaacc ggtaagagtg gtgactatcc
      181 cggttccagc gaggattcag ggctattcgt ggcgaagcac cgccagcggt ggttgaccga
      241 gtaaacggta ggtgctgaga aacccgacca gcatggtgag agagatcgtt gctgtaaagc
      301 ccatgacgag aacggcaggt tgaagcctcc aggagagatc gaacaccgtc gatagaacgg
      361 cccaggacag tgcgctggcc aatgcgcatc ccaggagccc gcccagcgct cccaggagcg
      421 cgtactcgac cgcaaacgaa cgcacgatca cgccgcgagt cgctcccagg gctttcagaa
      481 tgactgattc gtacaaccga cgatagcgcg ttgccgccaa ggccgctgcc attaccagac
      541 ctccggacag gacacagaac agcgccacgg cacggatggc gagggacagt cgatcaagca
      601 cccgtgcaaa actgtcgagg acgtcgctca tgttgatggc cgtcacattg ggaaaggatg
      661 ccacgaccgc ttgctgtaac gccacttctt cggatggcgc gacgtgaacg gtggccacaa
      721 aggtatgtgg ggctccatcg agagcgcccg gcgaaaagat catgtaaaag ttcgtggaaa
      781 agttccccca ttccacttgc cgaatgctgc tgatctctcc ggtgatgggt actccttgga
      841 tgtcgagctc gacggtgtct ccgactgtga gaccaagttg ctttgccgca tcctcctcga
      901 tcgagatcag cggtttggta aagtcttgtc ccggtttcca ccattcgcct tgcacaatct
      961 tgttgtcttt gggaaggtcc tggaggaacg tcaggacgta ttcgcgtgtc acgtaccatt
     1021 tctttcgccg ttcttccttc tgggctgctt tctctttctg ctcttcctcc tcggacgttg
     1081 cctcaaactt cacgggctcg tccttgacgg ctgatagccg ggatcgcacc agcggtgtca
     1141 accgagggtg tggatcactg gactgtcggc gcaggagagc ggccatccct tctgcctgat
     1201 caggttggat atcgatgaag aaaaacgtcg gggcgtcggt cggccgattg tcgcctactt
     1261 gtgtgaggag cgagcgctcg acgagcgata cggtcgtcac caccataacg gcgatgccga
     1321 tcgcaatcgt gatgctcacc gcctggctac cagggcgtac gacattgccc agcgcttgac
     1381 gaaggacaag gggcccaggg catggccatt tcttcagggc catgagcacc ccacgtgccg
     1441 aggtacccaa cagcaggacc gcagccgcga aggcgaggac aaagagcagc ccgaccttcc
     1501 aagacccggc ttgccatact gacaagagcg ccaatcccaa gccgatcgcg accgacacca
     1561 agatctttac cctgtcgagc ttgcgccaag cctcccacca tgctgccgtt ggatggggtg
     1621 tcgtcgcggt cagtggagca atctcacggc ggaagatcca agcaggtttg acatcacgga
     1681 tcgtcaacag cggccacagt gcaaagagca gcgtggacaa catgcccaat gccaacccct
     1741 ttgcaagagg agcgagggca agctccatcc cattcatcgt gaatcccaat tggtccaaaa
     1801 gatccgatgc cgtcaaggtc gcaatcatca atggaagtcc ttggtggaac agcacaccaa
     1861 gcatcaggcc gaccaaactt ccgaccagcc caagaatcat cgcctgtaac ccataggtcc
     1921 ggataatcgt ggaagactct gcaccgaccg tctttaaaat agcgatcgta ttcaactttt
     1981 cccgcacaaa ggcatggacc gacgtcgcaa ccccaaggcc tcctacaaac agtgccgtca
     2041 atccgatcaa tcctaaataa cgggtcagct gttccaaaga ctgcttcaat tgtggttgtg
     2101 cgtctcggta actcgacacg cgggcagagt ccggtgcaag ttgccctctc aactcgtaga
     2161 gcaacggttc aggtgccatc gtgcttggaa ttttcagtaa atatcgctca cgaatcctgc
     2221 tacccatctt gaccagttca gccgtgagga gtccttctcg tgacatcagg actcgaggac
     2281 ccaggctgaa ggcattggcc atgcgatccg gttccgtctt gactatgccg gtgatgatga
     2341 acagaccttg tccgatcttg agtggatctc ccaccgcgag tcccatgcgg atcaggagag
     2401 actcctgcac caccactcca aaacaggtgc ggtctggaca ccggcgagct tgccggtcaa
     2461 gaagacccac gaggccgccc tgtgcctcca ggcggagggt accatacagc ggatactgag
     2521 gctcgacggc tttgagttcg atgatttgag tcgactgccc actcccggac gatccggttc
     2581 tcgaggccat cgcgacgagc tcgctgacgt gggtgagagc gactccacga tcgctcaatg
     2641 agtccagaac ttcccggcct ttggggctca tcaggcgaga gagtcggatt tcaaggtcgc
     2701 ctccgagaag gcctctcgct tctttggtca cggctcgctc tacctgcgtt ccgaaaagag
     2761 agaccccggt caaggcgccc acgccgatcg ctatgcagac caagaaatag agaaagtgtc
     2821 gccatgccgc gcgtgtctct cgccacgcca tgttgaacat gaaggaaatc atgacccgaa
     2881 tatggggaag gaatcggaag atgacggatt acgggctggt gctcgtttcc cacttggcga
     2941 ggctgccaag aaatcaccga ggaacttcga agatgcaggt atactgtcgg actccagatg
     3001 tccgtcgtgg agcgacagga cacgctgcat cgatgaggcc agcactgcgt cgtgtgtcac
     3061 cagcacgagg gtggtgccat aatcgcgatg gagcgagagc agcaactcga tgacgtgaga
     3121 tccggtagaa ctgtcgagat tccccgttgg ctcatccgcc aataagatag gcggtcggca
     3181 ggcaaaagcc cgggcaaccg cgactcgttg ttgttctccc ccggataact gaacaggata
     3241 gtgccccata cggtcggata gcccgaccgc gacaagcagt tccgctgccc gatcgccggt
     3301 ccgagtcact ccgctgagtt ccagtggaac ggcgacgttt tcgattgccg taagggtcgg
     3361 gatgagatga aacgattgga agatataccc aaccttttcg cgccggaagc gggccatctg
     3421 gctctcaccc atggtcgtga tgtccgttcc atccagctga attgacccag tggtcggccg
     3481 atcgaggccg gccatcaaac caagcagcgt cgacttgcca cttcctgatg gccccacaat
     3541 ggccacagtc tgcttggcgg ggatgtcaaa ggtgatgcgg tcaaggatcg tgacggaacg
     3601 acttgccgcc gccaaggcca tcgaaacatc tttcacggtg atcatcaaaa acctcatttg
     3661 cctttcgaga taacaggacc aggaatgtta ttatactgta cagagtatat gatcgagtat
     3721 tgctccggtg aaacgatttc atgacatact tgtgccgacc ttcttgatgc ttctttggcc
     3781 gctcacgtcg ggggcgatct cttcttcagc atcggacaat agaccacgca tcgtggcatt
     3841 tggtgacagt ctcactgcgg gatttggggt gcaggccgat gaatcttacc cggctcaact
     3901 tcaacgccga ctggacagcc tcggctacca ctataggatg atcaacgccg gtgtgagcgg
     3961 cgacacgaca gccggtggac ttcgacgagt accctggatc ttaaacagca agcctgagct
     4021 ggtgattctc gaattgggtg cgaatgacgg gcttcgaggg ctcccggtcg atcaaacaaa
     4081 aagcaatctt cgtcagatca ttcgacaatt acaggaagcc ggtacgacgg ttgttttggc
     4141 ggggatgaag ctgccgccga attacggaca ggactatacg acaaattttg aagccatgta
     4201 ccagatgctg gccaaagaat accaccttgc cttgatcccc ttctttctcg aaggggtagg
     4261 cggttcatcc tcgttgaatc aggctgacgg cattcatccg actaaggaag gttatgagat
     4321 tatcgtaaac
//
LOCUS       DATMTI010000796         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_666218, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000796 DATMTI010000000
VERSION     DATMTI010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666218"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VN039_12615"
     CDS             <1..212
                     /locus_tag="VN039_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46839.1"
                     /translation="QAVAARLREMAQRFTGGGPVFYPAGDKRYTDTNNGQGGPQHIFQ
                     VGIDDFPTFSIFWPPTDLPPHNVYP"
     gene            220..615
                     /locus_tag="VN039_12620"
     CDS             220..615
                     /locus_tag="VN039_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46840.1"
                     /translation="MTNTQAITEQYPWVHLCNHVIEVTTWKGHDAHGLPVLDPTTTRR
                     YRCLIQENEVTSWSDIGGTDGMPYLAYILSIPITGSVTDDTVPIRKNDQITVVTPSYL
                     ASEKPRRVGTIKSYFDQYGNLHNIAITFE"
     gene            625..1020
                     /locus_tag="VN039_12625"
     CDS             625..1020
                     /locus_tag="VN039_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46841.1"
                     /translation="MGLSLNGLEEFRAAFERLRDRSRQELDSTVKRHMEDDVLPKTQE
                     KVPIATGALKGTGRVESGSKNGSYAVWYGDSAVNNDSMVDYAAAVHEIEHARHDPPTQ
                     AKFVQEPLEESVPRLVKSAGDALERLARG"
     gene            1023..1454
                     /locus_tag="VN039_12630"
     CDS             1023..1454
                     /locus_tag="VN039_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46842.1"
                     /translation="MGMMTADISAYILANIGAPWVDPATDSTNGNLYQQFYPPSSPDR
                     MCCVYERVGAPPQRSLGGVIAWHNTRLVVMNRCAASDGFAVAQADAFSIFNLLKTVVN
                     QTLSGTFYMEILPDGEPEAQSLDPSTRPIFITNFKVMKRTS"
     gene            1483..1665
                     /locus_tag="VN039_12635"
     CDS             1483..1665
                     /locus_tag="VN039_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46843.1"
                     /translation="MARQYRARNVIDYVSTRTGSYVHVEPGDVFDDMSTLSVRLELEH
                     ENIEEYDEKKDGGEKK"
     gene            1662..2420
                     /locus_tag="VN039_12640"
     CDS             1662..2420
                     /locus_tag="VN039_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46844.1"
                     /translation="MTALAASHGSNADVLVNGNIVSQYLNDWAQSMSRDKAETSAFKQ
                     LFKSYVAGLADNAITFAGLFDSSNPTAEDPLIYSYLTALTAVDNNWLYAPLGAQGASA
                     FGNTAYSVTGISTKYEVKSALNAANAITAEVQMDQNGGGLDRGQIYVPWSTQGAGGNS
                     TSIDYGSTSTANGGVLIVHTFSDTASLVVNLQDSADNSTFANVTGYTLSPTNATIAAY
                     RYPAAGVTPTGTVRRYTRITWTGTGTFLAMFARK"
     gene            2433..2870
                     /locus_tag="VN039_12645"
     CDS             2433..2870
                     /locus_tag="VN039_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46845.1"
                     /translation="MTALSASHGSRADFYLGTSGSPGTGVQISQYLTDVGLPFQRDKA
                     ETSAFKQMFKSYVAGLIDLAIPFSGIADVNIDGQLYNLLIMSGAGSAIQFQYAPLGVG
                     TTGSALWTGFGFLDKYESKSAINGAYSFTANFQSSTQPTRTIQ"
BASE COUNT          664 a          866 c          843 g          561 t
ORIGIN      
        1 atcaggcggt ggctgcgagg ctgcgagaga tggcacagag gttcaccggg ggcggtccgg
       61 tcttctaccc tgcgggagac aagcggtaca cggacaccaa taacggccag ggtggtccgc
      121 agcacatctt ccaggtaggg atcgacgact tcccgacgtt ctcgattttc tggccaccga
      181 ccgatctccc tccgcacaac gtctacccgt gaggctgcgg tgaccaatac ccaagccatc
      241 acagagcagt atccgtgggt gcacttgtgc aaccacgtca tcgaagtaac cacttggaaa
      301 ggccacgacg cacacggtct tcccgttctg gacccgacca ccacccggcg ttatcggtgt
      361 ttgatccagg aaaacgaagt aacctcgtgg tctgacatcg gtggcacgga tggaatgcct
      421 tacctggctt acatcctcag cattccgatc acaggatcgg tgacggatga caccgttccg
      481 atccggaaga acgatcagat aaccgtggta actccgtctt acctggcatc ggagaaaccg
      541 cgcagggtcg gcaccatcaa gtcttacttc gaccagtacg ggaacctgca caacatcgcc
      601 attactttcg agtgaggagg catcatggga ctttccttga acgggcttga ggaattccgc
      661 gctgcctttg aaaggctgcg ggaccgtagc cgccaagaac tcgacagtac tgtgaaacgg
      721 cacatggaag acgatgtgct gccgaagact caggagaaag ttcccatcgc cactggggca
      781 ctgaagggga ccggacgagt ggagtcggga agcaagaacg gttcctacgc ggtgtggtac
      841 ggggacagcg cggtgaacaa cgattcgatg gtggactacg cagcagcggt tcatgagatc
      901 gagcatgctc ggcacgaccc gcccacccaa gcgaagttcg ttcaggaacc gttggaggaa
      961 tctgttcccc ggctggttaa gagcgcgggg gacgcgctgg agagactggc caggggttaa
     1021 ccatgggaat gatgaccgct gacatctccg cgtacatcct ggcgaacatc ggggcaccgt
     1081 gggtagatcc cgcaacagac agcacgaacg ggaacttgta tcagcagttc tacccgccga
     1141 gttctccgga ccggatgtgc tgtgtctatg aacgtgtcgg tgctcccccg caaagatccc
     1201 ttggtggagt gatcgcctgg cacaacacac gtttggtcgt catgaaccgt tgcgctgcga
     1261 gcgacgggtt cgcagtggca caggccgatg ctttcagcat cttcaacctg ctgaagaccg
     1321 ttgttaatca gactctttcc ggaaccttct atatggagat acttccggac ggtgagccag
     1381 aagcacaatc tctggacccg agcactcgtc cgatattcat caccaacttc aaagtgatga
     1441 agagaacttc ataatccctg cgagtggcgc taaggaggat aaatggcaag acagtaccgg
     1501 gcgcgcaacg taatcgatta cgtgagcact cgcacgggtt cctatgtgca cgtcgagccg
     1561 ggagatgttt tcgatgacat gagcacgttg tcagtccgcc tcgaacttga gcacgaaaac
     1621 atcgaggagt acgacgagaa gaaggacggg ggtgaaaaga agtgaccgcg ctagctgctt
     1681 cccatggttc caatgccgat gtactggtga acgggaacat cgtcagccag tacctcaacg
     1741 actgggcgca gtcgatgtct cgcgacaagg ccgagacttc tgcgttcaag cagttgttca
     1801 agtcttatgt cgcagggctc gcagacaatg cgatcacctt cgccggtctc ttcgactcca
     1861 gcaaccccac tgccgaagac ccgctgattt acagctacct gaccgcgctc acggcggtgg
     1921 acaacaactg gctctacgct ccgctcggtg cgcagggtgc ttcggctttc ggaaacaccg
     1981 cttattccgt caccggaatt tcgacgaagt acgaagtcaa gtccgccctc aacgcggcga
     2041 acgccatcac cgcagaagtc cagatggacc agaacggcgg tggcttggac cgggggcaga
     2101 tctatgttcc gtggtccacg cagggtgcgg gtggaaactc cacttccatc gactacggtt
     2161 cgacttcgac ggccaacggc ggcgtgctga tcgttcacac cttctcggac accgcaagcc
     2221 ttgtggtgaa cctccaggac tcggcggaca acagtacctt cgccaacgtc actggataca
     2281 cgctgagccc cacgaacgcc accatcgcgg cttaccgcta tcccgccgct ggcgtgactc
     2341 ctacgggcac cgtacggcgc tacacccgca tcacgtggac tggcacggga accttcctgg
     2401 ccatgttcgc ccggaagtaa cgaggagatg agatgaccgc actttctgct tcccacggtt
     2461 cacgggcaga tttctatctc ggtacttccg ggagccccgg taccggtgtg caaatcagcc
     2521 agtacctcac ggatgttggc ctgccgttcc agcgggacaa ggcggagaca tccgcgttca
     2581 agcagatgtt caagagctat gtggccggtc tgatcgacct ggcgattccg ttctccggca
     2641 tcgcggacgt gaacatcgac ggacagctct acaacctgct catcatgagc ggggcgggca
     2701 gcgcgatcca gttccaatac gccccgttgg gcgtgggcac caccggcagc gcgctgtgga
     2761 ccgggttcgg gttcctggac aagtacgaat ccaagtcggc catcaacggc gcgtacagct
     2821 tcactgcgaa cttccagagc agcacgcagc cgacccgcac catccagtaa ttaccccagt
     2881 agcaaacttt tctgacaact acgaggaact aacatggcga actcgcgtac gaag
//
LOCUS       DATMTI010000797         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_284140, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000797 DATMTI010000000
VERSION     DATMTI010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284140"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="VN039_12650"
     CDS             <1..1514
                     /locus_tag="VN039_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV46846.1"
                     /translation="AQFRESFLIGLTILLFIGIFIFDIYTPTAFTDHALYIIVVLIAT
                     ASRFSWMPPLAAGAGTLLTVAGGLGTPWFTDLPRWIQMGNRSITIMVLWVLVWFAWKR
                     RQAKAALQKANEELEQKVAERTQELATVNRTLVTEIAEHVQTEQALRLSQGRLADILD
                     IAEDAIIVTDHDQSIMLFNQGAVKLFGYDSDEVLGNRIDLLLPERFRIDHPHYINAFA
                     HNPESARWMAQRREMFGLKKDGSEFPAEASISKLNLGERTTFTVIVRDITDRLRTERQ
                     LQSLTAELMTAQEEERRRIARELHDDVNQRLALLAIDMANMLSDPSTLTIRAKETIQS
                     LNQRLVRISDDVRRMAYQFHPSILDDLGLTAALKHMADEWSEKTGIKTVIVQEETTDP
                     LPRDIASCLYRVAQESLSNIMKHARAARVELELTCGDQEITLSIYDTGVGFDLKDIRA
                     RHPGLGLVNMRERVRSVQGRLDIQSQLGRGTHIIVHIPFSGAPHHEETTSSVS"
BASE COUNT          359 a          419 c          417 g          321 t
ORIGIN      
        1 atgctcaatt tagggaatct ttcctgatcg gcctcacaat cctgctcttc attgggatat
       61 ttatcttcga catctacacg cctacggcct tcaccgacca tgcgctctat atcatagtcg
      121 tgttgatcgc cacggcttca cgtttttcgt ggatgccccc tctcgctgcc ggagcgggaa
      181 cactcctcac cgtggctgga gggcttggga ctccctggtt taccgattta ccgcgatgga
      241 ttcagatggg aaaccggtcc atcacgatta tggttttatg ggtgctcgtc tggtttgcat
      301 ggaaacggag acaggcgaaa gcggccctac agaaggcgaa tgaagagctc gagcaaaagg
      361 tcgcagaaag gacccaggaa ctcgccaccg tgaatcggac cttggtcacg gaaatcgccg
      421 aacacgttca gaccgaacag gcgctgcgtc tctcgcaggg acggcttgcc gacatcctgg
      481 acatcgccga agacgccatc atcgtcaccg accatgatca atcgatcatg ctctttaatc
      541 aaggggcggt gaagctgttc ggctacgact cggatgaagt gctggggaac cggatcgacc
      601 tgctgttacc cgaacggttt cggattgatc atccacacta catcaacgcg ttcgcccata
      661 accctgaatc ggctcgttgg atggcgcaac ggcgtgaaat gttcggcttg aaaaaggatg
      721 gaagcgagtt tccagcggag gcgagcatct caaagctgaa ccttggcgaa aggacgacgt
      781 ttacggtgat cgtgcgggac atcacggacc gattgagaac agaacggcaa ctccagtcgc
      841 tcaccgccga actgatgacg gcgcaagaag aagaacgccg gcgcatcgca cgcgaacttc
      901 acgacgatgt gaatcagcgg ctggctttgc tggcaatcga tatggcgaac atgttgtccg
      961 atccatcgac gctgaccatc cgagccaagg agacgatcca atccctcaat caacggttgg
     1021 tgaggatttc cgatgatgtc cgccgcatgg cctatcaatt ccatccctct atcctggacg
     1081 acctgggcct gactgccgcg ctcaagcaca tggccgatga atggtcggag aagacgggga
     1141 ttaaaaccgt catcgtacag gaagagacaa ccgatccttt gccgcgcgat attgccagtt
     1201 gtctctatcg ggtggcgcag gagagcctga gcaacatcat gaaacatgcc cgcgccgcac
     1261 gcgtggaact cgaattgacc tgcggcgacc aagagattac gctatcgatt tatgataccg
     1321 gtgtcggctt cgatctcaag gacattcggg ctcgccatcc cgggttgggt cttgtcaata
     1381 tgcgggagag ggtgcggtcc gtacaaggcc gattagacat tcagtcccaa ctaggccgag
     1441 gcacacacat cattgtgcac attccattct ctggagcgcc acatcatgaa gaaaccacga
     1501 gttctgttag ctgacg
//
LOCUS       DATMTI010000798         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_454408, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000798 DATMTI010000000
VERSION     DATMTI010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454408"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VN039_12655"
     CDS             <1..1124
                     /locus_tag="VN039_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46847.1"
                     /translation="EGESESPGGGEDLQEDDAGDSDGRNVGDSPDADDPDMDFDGGGR
                     DGGDDSDGLPEDGSSSTSSNGAGTGAGRDQDFELDARELAEELAETLAEVLESEHVQN
                     DVEQLQDAMADQAGLNSTLDRRTGLSMAPVTPEMVNQSERLASVLREMWAQMEPGWEY
                     GLSEGNRIDMNRAAMIRNVEDYDSIYVDWSEGQQDNSGLCAVVLVDKSSSMSGSVYAK
                     EAGYLGTRANVVSKNLWEIRKALDEIDATVTVIDFDYECCTMYDTDEKADPSQYANMY
                     PSGGTNPHKAIEEARRILSMTDMPNKLLVILTDGEWGGNQRDILYSGFPDVVKVAILI
                     GNLRFDYTSYFDVTARTSGDIFDPIAQAVVAITERSMNR"
     gene            1121..1576
                     /locus_tag="VN039_12660"
     CDS             1121..1576
                     /locus_tag="VN039_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46848.1"
                     /translation="MSTHTDSRSMIDNRRLLSGLKRKLADLKRLSILKSQTESQYKEF
                     SAPALSELVEADGFNGRGVRFELDGVEYAAIACQPTPGKLWNAEPLIEWLKKNGHWSN
                     VSTEVLDPHKLQAEIAAGNIPQRSVAKFQIDEEPKKPYVKFINPKPDSQ"
     gene            1573..1707
                     /locus_tag="VN039_12665"
     CDS             1573..1707
                     /locus_tag="VN039_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46849.1"
                     /translation="MNGTQEAIVWIVGIVVGGLTISEFAGALRARWTKNTEIEEDENL
                     "
BASE COUNT          437 a          464 c          605 g          312 t
ORIGIN      
        1 aagaagggga atctgaaagt cctggcggtg gggaggattt acaagaagac gacgcgggag
       61 attctgacgg acggaacgtt ggtgactcgc cggacgcaga cgacccggac atggactttg
      121 atggaggggg acgggacggc ggagacgatt ctgacggact tccggaagac ggttcttcct
      181 ccacttcctc caatggagct ggtactggag ccgggcgaga ccaggacttc gaactagacg
      241 ccagagagct agccgaagaa ctggcggaga cgctggccga agtcctggag agtgagcacg
      301 tccagaacga cgtagaacag ctccaggacg ccatggcgga ccaagccggg ctcaacagca
      361 ccttggaccg tcgtacgggg ttgagcatgg ctccagtgac gccggagatg gtcaaccaga
      421 gtgagcggct ggcctcggtg ctgcgggaga tgtgggcgca gatggagccc ggctgggaat
      481 acgggctctc cgagggcaac cgcatcgaca tgaaccgcgc ggcgatgatc cggaatgtag
      541 aggactacga ttccatctac gtcgattgga gcgaggggca gcaggacaat tcagggctct
      601 gcgctgtggt tttggtggac aagtccagtt ctatgagcgg ttcggtctac gccaaggaag
      661 ccggatatct gggaacccgt gccaatgtgg tctccaagaa cctctgggaa attcggaaag
      721 cactcgacga gatcgatgcg acggtaaccg tcatcgattt tgattatgag tgctgcacca
      781 tgtacgacac cgacgagaag gccgacccca gtcagtatgc gaacatgtac ccgagtggcg
      841 gaacaaaccc tcataaagct atcgaggaag cccggcgcat cctgtctatg acggacatgc
      901 cgaacaagct tctggtgatc ctcacggacg gcgagtgggg cggtaatcag agagacattc
      961 tctactcagg attcccggat gtggtgaagg tcgccattct gattggaaac ttgcggttcg
     1021 attacacgag ctacttcgat gtgaccgcga gaacaagtgg agacatcttc gaccccatcg
     1081 ctcaagcggt ggtggcgata accgagagga gcatgaacag gtgagcacgc acactgattc
     1141 gcgcagcatg atcgacaacc gtcgtctgct gagcggcttg aagcggaagc tggccgatct
     1201 caagcggctg agcatcctga agtcgcagac cgaatcgcag tacaaggagt tctctgcacc
     1261 ggctttgtcg gagctggtgg aggcggacgg gttcaatggc cgtggagtcc ggttcgagct
     1321 ggacggggtg gagtacgcgg ccatcgcttg ccagcccacg ccgggcaagc tctggaacgc
     1381 ggagccgctg atcgagtggt tgaagaagaa cggccactgg agcaacgtct ccacggaagt
     1441 tctcgacccg cacaagttgc aggccgagat cgcggcggga aacatccctc agcgttcggt
     1501 tgcgaagttc cagatcgacg aggagccgaa gaagccatac gtgaagttca tcaatccgaa
     1561 gccggacagc cagtgaacgg cacgcaggaa gcgatcgtct ggatcgtcgg aatcgtggtc
     1621 ggcggcttga ccatcagtga attcgccggt gcgcttaggg cgcgctggac gaaaaacact
     1681 gaaatagaag aagacgagaa cctgtaggta gcctgtgcgg ctcggcggaa actccgagcc
     1741 gtgcggaagt cccacagaca ggaggaggga atagtggcca aggtggttcg cgtcaagagg
     1801 aagcctgaac cagctaag
//
LOCUS       DATMTI010000799         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_157366, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000799 DATMTI010000000
VERSION     DATMTI010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157366"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VN039_12670"
     CDS             complement(<1..280)
                     /locus_tag="VN039_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46850.1"
                     /translation="MSDETLLEFITRHEGKRNRVYDDATGDILKPGYTLVGHPTIGVG
                     INLEEPISDATIQQMLREKCNDAINECLHAFPWYAELTEPRQWAVLDMR"
     gene            complement(401..>2269)
                     /locus_tag="VN039_12675"
     CDS             complement(401..>2269)
                     /locus_tag="VN039_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46851.1"
                     /translation="LNHTPQNSTLRDIAFIDWGSASANAIRVSDGVNIVVDHITVLGT
                     GPGANGVQTDDAATGVPAATNSIAMTNLLIAGITPGRGFNITGFNTWSGNEIFSTGNG
                     TAFNPALPANWTNTSTSAHGMGTCKVWIPTGAAVKGAGTGGSDIGATILYRYVDGVLT
                     TTPLWDPVTGAFPFGPADSDGTNRVAGDSLFDFHTRININSGGCSFPAGYGAGGGSGG
                     TVTIGGTASGTGANSLSFPFTVSAGQDRALVCFGMRDDGSNVGSIAAADISGQALSFV
                     KRSVTSPGWRAVEIWQVANPISGSRTVNATTTGAVSGMAGQAMQFDTTSALNTAVSAS
                     SPPNTPTSTIQVTAPTNTNELVVDCVASSSGVSITAGANQTPYPTVTHPSISIALGSS
                     TQPGTFGGVMDDTLGGTVYAAQVAISLVASPADTPSTATLTLTQFVIRELFGAEAIDG
                     SKPLPPHDITATPQDALTATANHQAYIGTTGAFRVRTKITGSVATTSPFGVQLACRVN
                     AEARTPVQTTFNGKVFRLYGSDPDPDIPASLTSTTNQLCTSNCNPGAFLRDLSPYVVI
                     ALPDGNSIELEWAIQAQASVGDVIQCAPQKDDGTPLDVETVVPTVVVIDNQSAVGN"
BASE COUNT          468 a          653 c          641 g          507 t
ORIGIN      
        1 agcgcatgtc cagaacggcc cactgacgcg gctccgtgag ttctgcatac caagggaaag
       61 cgtgtaagca ttcattgatc gcgtcattgc atttctcgcg aagcatctgt tggatcgtgg
      121 catcggaaat cggctcttcc agattgatcc cgaccccaat cgtgggatgc ccgacgagcg
      181 tatacccagg cttcagtatg tcgccggtcg catcgtcata gacacggttg cgctttcctt
      241 catggcgcgt aataaattcg agcaatgttt catcgctcat cagtggaccg ccttttcttc
      301 ttgtcggtcg tccatccaca gtcacacttc cacggctcgt gcaaagtttc cttttcaagc
      361 ttctgcccgc acatgggaca tgatcgttgc cacacttgca tcaattgcct acggctgatt
      421 ggttatcgat cacgacaact gttggaacaa ctgtctccac atcgagcggc gttccatcgt
      481 ctttttgcgg ggcgcattga atgacatcgc cgaccgaggc ctgcgcctga atcgcccatt
      541 ccagttcaat gctattgcca tccgggaggg caatgactac atagggcgag agatcgcgga
      601 ggaaggcccc aggattacaa ttagaggtac acagttggtt ggtcgtcgat gtcagtgagg
      661 ccggaatgtc tggatcgggg tcagatccat acaaccgaaa gaccttgccg ttaaaggtcg
      721 tctgcacggg cgttcgcgcc tcggcattga cgcggcaagc cagctgcacc ccaaagggcg
      781 aggtcgtcgc cacgctgccg gtaattttgg tgcggactcg gaatgcgcct gtcgtcccga
      841 tgtaggcctg atggttcgcc gtcgctgtca gtgcgtcttg gggagtggcc gtgatatcat
      901 gcgggggcaa tggctttgag ccgtcgattg cttccgctcc aaacagctca cgaatcacaa
      961 attgagtcag cgtcagcgta gcggtactcg gcgtatccgc tgggcttgcg accagtgaga
     1021 tcgccacttg tgcggcatag acggtgccgc ccaatgtatc atccatgact ccgccaaagg
     1081 ttcctggctg cgtggacgat cccagcgcaa tcgagataga gggatgcgtt accgttggat
     1141 agggcgtttg attggccccg gcagtaatgg agacaccgct tgacgaggcc acacagtcca
     1201 ccacgagttc attggtattc gtcggcgccg tgacctgaat cgtggaggtg ggcgtattgg
     1261 ggggagagga cgcactgacg gccgtattca acgccgaggt cgtatcaaat tgcatggctt
     1321 gtccggccat gccgctcacc gcgccggtcg tcgtcgcatt gaccgtccga gatcctgaaa
     1381 tcgggttcgc cacctgccag atttcgacag ccctccaccc tggcgaggtg actgaccgct
     1441 tgacgaatga cagggcttgt ccgctgatat cggcggcggc gatggaaccg acattgctcc
     1501 catcatcccg catgccgaag cacactaagg cccgatcttg tccggcactg acggtaaagg
     1561 gaaaactgag gctattcgca ccggtgccgg aggccgtccc tccgatggtg accgtgccgc
     1621 ctgagccgcc ccctgcccca tagccagccg ggaacgaaca gccgccgcta ttaatattaa
     1681 tccgggtatg gaagtcgaag agcgaatccc cggccactcg attagttccg tctgaatcag
     1741 ctggcccaaa cggaaaggct cccgtcacag ggtcccacag cggcgtcgtc gttaagacac
     1801 catccacata gcgatagaga atggtcgcgc cgatatcgct tccgccagtt cctgcccctt
     1861 tgacggctgc gccagtcgga atccagacct tgcaggttcc catgccgtgt gcactggtgg
     1921 atgtgtttgt ccaattggcc ggcagcgcgg gattgaacgc cgtgccattg ccggttgaga
     1981 aaatttcatt gccagaccac gtattgaatc ctgtgatgtt gaatccccgt cccggcgtaa
     2041 taccagcaat aaggagattc gtcatcgcaa tggagtttgt cgctgccggt actcccgtag
     2101 ccgcatcatc cgtctgaacg ccattcgcgc ccggtccggt tccgaggacc gtaatgtgat
     2161 caactacaat attgacccca tcagagacac gaatcgcatt ggctgatgct gatccccaat
     2221 cgatgaatgc aatatctctc aacgtggaat tttgaggggt atggttgag
//
LOCUS       DATMTI010000800         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_284264, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000800 DATMTI010000000
VERSION     DATMTI010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284264"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..465)
                     /locus_tag="VN039_12680"
     CDS             complement(217..465)
                     /locus_tag="VN039_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46852.1"
                     /translation="MQSQTQVVGIEGGDQNGLAALRVVGPIPDGVVALPLDLTTNVVG
                     GEALTFIGYVPRGPMESLAAGGPDTQGASGQSSGARHS"
     gene            complement(434..688)
                     /locus_tag="VN039_12685"
     CDS             complement(434..688)
                     /locus_tag="VN039_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46853.1"
                     /translation="MAIKLHATWLLLGALIAPLLLWADANLDYQNRGDRFEGVKPKPV
                     SGYDIEVISVLADYREPAERLPDQLRVGFYFAEPDPGRRD"
     gene            complement(828..1850)
                     /locus_tag="VN039_12690"
     CDS             complement(828..1850)
                     /locus_tag="VN039_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HWV46854.1"
                     /translation="MALSYHIAVAEKKVKRAPLPKGVRRRGKPFGSSTGLASIELQAA
                     APPGVVAELHQAVEQDGGKVLSVYRDPYGGQWLVLAALPIELVEPTPYQRNLSDSHVR
                     KLEAAIGKIGRFLDPIITVRIAKPGHMAKYWTPNGNHRLSAMRTLGAKSIIAIVVPEA
                     AAAYQILALNTEKAHNLREKALEVIRMYRELAQLDHATEAVYALEFEEPALITLGLCY
                     EGRPRFSGGAYHPVLKRVDEFLQKPLHAALSIRQQHAKTVLALDDMIVKQVETIKAKG
                     LTSPYLKSFVVARVNPLRFRPKDEPPLSFDDALDRMTQAAEKFNPDKIKMDDLAKSGG
                     VPDENE"
     gene            1849..2541
                     /locus_tag="VN039_12695"
     CDS             1849..2541
                     /locus_tag="VN039_12695"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWV46855.1"
                     /translation="MANESPNRKILRIGHRGACGHAPENTLASIEQAIALQCAFTEVD
                     IQRTSDDALVLLHDKRVDRTTNGRGRLSELTLPDIRKLDAGGGQMVPTLEEALRAANG
                     RIGVILELKTGGLAYDVYATVRASGFNRPVIYASFLQKELHHVRRVDPNSQTLVLFNK
                     WFPKDPVAAALKLQATHVGLHFNTATRHRVNALHNAGLTVFVYTVNKPAEINKMNLLG
                     VDGLISDFPDRI"
BASE COUNT          710 a          946 c          902 g          698 t
ORIGIN      
        1 gcatttattg tcagcaagac tcatggaaat ctcctttcat gtataacaag aaaacattgt
       61 aaaggggctt tgagaggaag ggcaagacgg aagaaatcaa cccaaactca ttgtcctttt
      121 ctgacatctt ccatgcagcg aaaacctctc ccaggcgctc catcataata ggcgcctgcg
      181 tacatgcgca cctgataatc gaggtggccg acagcatcac gaatgccgag cgcctgaaga
      241 ctgccccgaa gcaccttgcg tgtctggtcc tccggcggcg agggattcca tcgggcctct
      301 tggtacgtag ccgatgaatg tgagcgcctc accgccgaca acgtttgtcg tcaaatccag
      361 cggcaatgcc accacgccgt ctggaatcgg cccgaccaca cgcagggccg cgaggccgtt
      421 ttgatctccg ccttcaatcc cgacgacctg ggtctggctc tgcaaagtag aatccgactc
      481 ggagctggtc aggcagacgt tcagcaggct cgcgataatc tgcaaggacg gagatgactt
      541 cgatgtcgta gccggaaacc ggtttcggtt tgactccctc gaagcggtcg ccacgatttt
      601 ggtaatccag attggcatcg gcccacaaca ggagaggggc aatcagcgct cccaagagaa
      661 gccacgtcgc gtgaagcttg atcgccatga tgaggtccgc tgactccagc gaattgctga
      721 gtcatccctc gaatccggcg gcagtatagc ccagaggcct aagtgaatct agctatgcag
      781 gggcaagaca gtggcgaacg aatgccggca tctaaggtag gaacgggcta ctcgttttcg
      841 tccggaaccc cgccggattt ggccagatcg tccatcttga ttttgtctgg gttgaacttc
      901 tccgcggcct gcgtcatgcg atcgagcgcg tcatcgaacg acaagggcgg ttcatctttg
      961 ggacggaacc gaagggggtt cacgcgggcc acgacgaagc ttttgagata gggactggtc
     1021 aaccccttgg ccttgatggt ttcgacttgt ttgacgatca tgtcatccaa tgcaaggacg
     1081 gtcttggcgt gctgctgacg aatgctcaga gcggcatgca acggtttctg tagaaattcg
     1141 tccactcgtt tcagcaccgg atggtaggcg ccgccgctga accgaggccg tccttcataa
     1201 cagagcccca gcgtaatcag cgccggttcc tcgaactcca aggcatagac agcttccgtt
     1261 gcgtgatcga gttgcgccag ctcgcggtac atccggatca cttccaacgc tttctcacgg
     1321 aggttgtgtg ctttctcggt attcagcgca agaatctgat aggcggcagc agcttctgga
     1381 accacgatcg cgataatgct cttcgctccc agggtccgca tcgcggacag gcgatggttg
     1441 ccgttcggtg tccagtactt cgccatatgg cctggttttg cgattcgcac ggtgatgatg
     1501 gggtcgagaa agcgaccgat tttcccgatc gccgcttcca actttctgac gtgagagtcg
     1561 gaaagattcc gctgatatgg tgtcggctcg acgagctcaa tcggcagggc cgccaagact
     1621 aaccactgtc ctccatatgg gtcacgatag accgagagaa ctttccctcc atcttgttcg
     1681 accgcctgat gcaactccgc cacgacaccc ggcggtgcgg cagcttgcaa ctcaattgaa
     1741 gcaaggccgg tcgaggatcc aaacggtttc ccacgccgtc tcaccccctt ggggaggggt
     1801 gctcttttca ctttcttttc agccacggcg atatggtagg acagagccat ggcaaatgaa
     1861 agccccaatc gaaagattct ccgcatcggc catcgcggcg cctgcgggca cgcaccggaa
     1921 aataccctgg cgtccattga gcaagcgatc gcgctccaat gtgccttcac tgaagtggac
     1981 attcagcgaa cttccgacga tgcgctggtc ctcctgcatg acaagcgggt agaccgcacc
     2041 acgaacggtc gcgggcgtct ttctgagctg acgcttccag atattcgaaa actggatgcg
     2101 ggtggcggcc agatggttcc gacgttggag gaggctctca gggccgccaa cggccgaatc
     2161 ggcgtgattc tggagctgaa gacggggggg ttggcctatg atgtctatgc gaccgtccgt
     2221 gccagcggct tcaatcgacc tgtgatctat gcttcatttc tacagaaaga acttcaccat
     2281 gtccggcgag ttgatccgaa ctcccagacg ttagtcctgt tcaacaaatg gtttcccaag
     2341 gaccctgtcg cagcagccct caaactacaa gccacacatg ttggtctaca cttcaacacc
     2401 gctacacgcc atcgagtgaa cgcactccac aacgcggggt tgaccgtatt cgtctacacc
     2461 gttaacaaac ctgccgagat caacaagatg aacctcctcg gtgtagacgg tcttatctcg
     2521 gatttcccgg atcgcatctg atcggcacaa cgaggggaag ggttcagaaa cagcgcgggc
     2581 gaccttgcac tacggtggga ggtcgcccgc gatttggcgc ttaggacgcc ataataatat
     2641 tattgatgtc tgctgtcgcg gccctcaaga cccgcacgat actgtgggtg acccaaggtt
     2701 tccaatcggg ggagacctcg tcaatcccag gtgcttgtgt ccctctgctg ccgaaaattt
     2761 ctccgccaac aaatggcttt caccctgcgt aagggcgcta cgtgccgatc cggcacagcc
     2821 ggcgaagaac cggtcagctc gaccgacgag aaacccgccc ttgcatcggc gggcctgatg
     2881 ggccaggtgg cgtcgtgtcg tccatctcca ccctcctctc gcgatgaagt caccgagaga
     2941 cctggacggc agccgcgcgg tctacccgat ctctcgaact tcggttgata gatgggccgc
     3001 atttcttccc tacgctcttt gcaaaacctt gtcaagtgtc actcagcttt ttcttggcac
     3061 tgatgcgtaa ggcatagcag atgccaacag gcgtaaggtc ttcgagccca ggaccacgcg
     3121 tgaatcgggg cactgacgag attacggctg cttctgaaca tcctgagcac agcggaagcc
     3181 cacattcgga tcatgctcgg tcggatcatc cttcgtgcga aacgacgacc gcagacgata
     3241 cggggtattg atatag
//
LOCUS       DATMTI010000801         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_794582, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000801 DATMTI010000000
VERSION     DATMTI010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794582"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VN039_12700"
     CDS             complement(<1..455)
                     /locus_tag="VN039_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46856.1"
                     /translation="MNWEAISAIGQIVGAIAVVVSLIYLAREVRSSAHAAQLASRHSM
                     EDIFIRWSQQLADNQNLRELYYRGLHDFESLEGADLVGFGTLIQGLFRLWEEAYYRHL
                     EGHLDARVWYGWEAAMNDLNGYPGVQGWWRSRSHWFHEDFAKHINQLQQT"
     gene            complement(705..778)
                     /locus_tag="VN039_12705"
     tRNA            complement(705..778)
                     /locus_tag="VN039_12705"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(742..744),aa:Asp,seq:gtc)
     gene            1323..>1733
                     /locus_tag="VN039_12710"
     CDS             1323..>1733
                     /locus_tag="VN039_12710"
                     /inference="COORDINATES: protein motif:HMM:NF036798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HWV46857.1"
                     /translation="MSDERSEGLQGEGDASSETLLDSFDQMIGSLPPGDPVRHDLLQL
                     RPQILDQQETMVEARRMIEKLEEVVKKVTAPANRIGTFLGATSKETAHIVVGGADYYC
                     NVDPRISLSRLKRGTRVLVNEAFVIVGDLGFETAG"
BASE COUNT          427 a          448 c          474 g          384 t
ORIGIN      
        1 gtctgctgta gctgattgat atgcttcgca aaatcctcat ggaaccaatg cgaccgtgaa
       61 cgccaccaac cctgaactcc cggatatccg ttgaggtcgt tcatggccgc ttcccaccca
      121 taccacacgc gtgcatccag atgcccctcc aagtgtcgat aataggcttc ctcccagagt
      181 cgaaacagcc cttgaatcag tgtaccgaag cctacgagat cagcgccttc gagagattcg
      241 aagtcgtgga gaccgcgata atacagttct cttagattct gattgtctgc gagttgttga
      301 gaccaccgaa tgaagatgtc ttccatcgaa tgcctcgaag ctagttgcgc agcgtgagca
      361 ctgctacgga cttccctcgc caaataaatc aacgaaacga cgacggcaat tgcgccgacg
      421 atttggccga ttgcactaat cgcttcccaa ttcacggcat gctctaacta tgtttggacg
      481 aatcttcgta aacgtcacga agatttcgtt gcgccgccac actgcgtaaa tgcgtgcagg
      541 aattgttgat gacatctgcg ctcctctgga ctcaatcgaa acgccgaagc gattctggtg
      601 cgatcctgag cgcgagccat gcgaaggcgg aagcgaactg caatttgaca gaaacttgtc
      661 ataaaccgga tccctgattt ggcgtttgtt agggaaaagg ggaccggagc ctacggggct
      721 cgaacccgca acctccgccg tgacagggcg gcgctctaac caattgagct aaggctccca
      781 agcaaatccg aacgtagcgt gtggaggtgc acctgcaact cgcgcgaaca tggtgtttcg
      841 aaaagaaaga cggctcggga aatttcaacc tccaaattcc aaacacttgc aaatgcgaaa
      901 tggcttcgca ggaacaaaca tctctggtca tcgctatgca cgtccacttc cgggaatgac
      961 ctgacattga aagcctggct tcgccccgaa ttgctttcgg gctgcgctgc ggcttcgctt
     1021 cgctcagaat gacagtcgta ggaatgctgg tgctattgac ggctatacac gcttctgcgc
     1081 acgagctcga atcgccggcg cacagaacac gaactgctgc aggcttggac atgtgatcct
     1141 gggacgtaaa tccttggtcg ttgggaattg gaactcggaa tttccgcgac cttagaagtc
     1201 gcggtcggtt gaacatttga agacgcgaag cgtcgaattc gaaaaggttt cgtgagtgta
     1261 cattgcgaac aggattgatg acggtataat taagtcggtt cggcggattg acccaaacca
     1321 taatgagcga cgagcgcagc gagggattgc agggcgaagg agacgcgagt tcggaaactc
     1381 tcctcgattc atttgatcaa atgattggat cattgccgcc tggcgatccg gtcaggcatg
     1441 atctgttgca gttgcgtccg cagatcctcg atcagcagga aacgatggtc gaagcgcgcc
     1501 ggatgataga gaagcttgag gaagtggtta agaaagtaac tgctcccgcg aaccggatcg
     1561 ggacctttct cggcgcgact tcgaaggaaa ccgcacacat cgtcgttggc ggcgcggatt
     1621 attattgcaa cgtcgatccg cgcatatcgc tgtccagatt gaaaaggggc acgcgcgttt
     1681 tggtcaacga agctttcgtg atcgtggggg atttgggatt cgagacagca ggc
//
LOCUS       DATMTI010000802         4488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_157476, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000802 DATMTI010000000
VERSION     DATMTI010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157476"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VN039_12715"
     CDS             complement(<1..1311)
                     /locus_tag="VN039_12715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWV46858.1"
                     /translation="MHISVIGSGYVGLVTGACFAEFGVHVTCMDSDSRRIEKLEKGEI
                     PFYEPGLGELVTKGVKEGRLSFTTDIAKAVDKALVIFIAVGTPPRGDGSADLSYVEEV
                     GKGIARHMTGYKVIVTKSTVPVGTGATLREVITTAQTNAFRFDLVSNPEFLREGSAIE
                     DFMRPNRVVIGADSDQAVAIMKDLYRPLYLIETPIVVTDVPTAELIKYASNAFLATKI
                     SFINEIANLCEKVGANVQMVAKGMGLDHRIGAKFLHAGPGFGGSCFPKDLAALIQTGE
                     RHGRPMQIASAASRVNDSQRGRMIDKIREALGGVKGKTLAMLGLSFKPNTNDLREAPA
                     LAIGRVLLADGASIRAYDPEALMEACRMLPELQPCRDTYHAAEGADALVIMTEWNVFR
                     NLDFEKLKSVMRVPVLLDLRNVYDPERVAAAGFKHVSVGRAAQTP"
     gene            1489..3036
                     /locus_tag="VN039_12720"
     CDS             1489..3036
                     /locus_tag="VN039_12720"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HWV46859.1"
                     /translation="MALHPPRIPRWFLLLTALVTGAVVMALEILGSRLLAPVFGSSLF
                     VWGALIGVILAAMSSGYAFGGWVSDRYTGGGVPAVLLAFSGSWTFLVAWANQPILFEI
                     EKMVQDPRWGPCLAATVLLAPPAFGLSGVLPAMLRLAVADMEHLGRQTGRMIALSTVG
                     SLAGTWGTAFFLLSWLGSQSLMAWLGGMQVGLGVLWIMKGTSARPLVLLIALGCFVLL
                     GAMALNPIKRLKTPVYQEESPYQQVRIREDDLFRYLVLDRTFHATMWKVDPTSLFLPY
                     SQMMVSSLALVSEPKRSLILGHGGGSLAKWLARHWPALELDIVEFDPVVVRMAEEYFD
                     YRPPANHHVFVKDGRAFLNATDHTYDLMWIDAFARDMIPFHLTTAEFYSLVRTHLNPE
                     GIVAVNLASSGKEGDLARAAAVVQTMKQAFPVLATFAVEGPWKTGMTPAKNLIFFGGR
                     LIEHESPDSLMAKITDMAMNQRLPMETIALLSTRRTEPWPPGVVLSDDFAPYDLLLGR
                     ERSQLVE"
     gene            3150..3293
                     /locus_tag="VN039_12725"
     CDS             3150..3293
                     /locus_tag="VN039_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46860.1"
                     /translation="MGILALSHEADIAKVQEWLGHANVSTIRLYDRRKTRPEDSRTFR
                     VRY"
     gene            complement(3508..>4488)
                     /locus_tag="VN039_12730"
     CDS             complement(3508..>4488)
                     /locus_tag="VN039_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV46861.1"
                     /translation="LNQLLVEMDGFDTTEGIILVAATNRPDVLDPALLRPGRFDRQVT
                     VNHPDLRGRSAILKVHTKKIPVAADVELEKIARGTPGFSGADLENLVNEAALWAARQN
                     KNEVGVVDFEMAKDKILMGPERKSMMLTDEEKRVTAYHEAGHALMAKLLPGTDPVHKV
                     SIIPRGRALGVTMQLPTDDRHNHSQEFLYATLAILLGGRVAEELVFKQVTTGAGNDLE
                     RATDLARKMVCEWGMSEKLGPLTFGQKEESMFLGRAFATKRDLSDAMALEIDLEIKRL
                     VTENYERTKHVLTEHMVTLKALADALLEKEVLDAMEIDKIISNSSLTVLA"
BASE COUNT          922 a         1300 c         1225 g         1041 t
ORIGIN      
        1 gggagtctgt gccgcacgcc ccactgacac atgtttgaag ccagcagccg cgacgcgttc
       61 ggggtcatag acgtttcgga gatcaagtaa gacgggaacc cgcatgacgg acttcaactt
      121 ttcaaaatcc aggtttcgaa acacattcca ctcggtcata atcaccaaag catcggctcc
      181 ttcggctgca tggtaggtgt ctcggcaagg ctggagttca ggcaacatcc ggcaagcttc
      241 cataagagcc tcaggatcgt atgcacggat gctcgcacca tcggcgagga gtactcgacc
      301 gatcgccaag gccggggctt ctctgagatc gttggtgttc ggcttgaagg agaggccgag
      361 catcgccaac gtctttcctt tcactccccc caacgcctcc cggatcttgt cgatcatccg
      421 ccctcgttga ctgtcgttca cccgtgaggc ggcggaggca atctgcatgg gacgtccatg
      481 gcgctcgccg gtctgaatga gcgcggcaag atccttgggg aaacacgacc ctccgaaacc
      541 cggccctgca tgaaggaact tcgccccgat tcgatgatcc aagcccatgc ccttggccac
      601 catttgaaca ttggctccga ctttttcgca aagattggcg atctcgttga taaacgaaat
      661 cttcgtggcc aagaacgcat tggacgcata cttgatgagc tcggcagtcg gaacatccgt
      721 cacgacgatc ggtgtttcga tcagatagag cggacgatag agatccttca tgatggcaac
      781 cgcctggtcg ctgtccgccc cgatcacgac gcgattcggt cgcatgaagt cctcgatggc
      841 cgacccttct cggagaaatt ccgggttcga aaccagatcg aaccgaaagg cattcgtctg
      901 agcggtggtg atgacctcgc gaagtgttgc gccggtgcct acggggaccg tggacttggt
      961 cacgatcacc ttgtatccgg tcatgtgacg cgcaatccct ttaccgacct cttcgacata
     1021 cgagagatcg gcggagccgt cacccctcgg cggtgtcccg acggcaataa agatgacgag
     1081 agccttatcg acagccttgg caatgtccgt agtaaaactc aacctgcctt ccttgacccc
     1141 tttggtgacc aattccccaa gccccggctc ataaaacgga atctcgccct tctccagctt
     1201 ttcgattctt cggctgtcgc tgtccatgca ggtgacgtgg acgccgaact cggcaaaaca
     1261 tgctccagtg acaagtccga cgtagccgct tcctattaca ctgatgtgca tgagccctcc
     1321 tcaatggcaa aagcagggga agtatagcag ccgatgcgaa tgcgagcaac gaatccacgc
     1381 ctacttgtcg cggcggtcct ttcgttgact cttggaaaac acggtgagta aaatcccacg
     1441 ctggtacagg cctgataagg agcccttctc aatccccccg ttcaatcgat ggccctacat
     1501 cctccacgaa ttccccgctg gtttttgctt ttgaccgccc tcgtgaccgg tgcggtggtg
     1561 atggcgttgg aaatcctcgg cagtcgactg ttggctcccg tattcggcag ttcgttgttt
     1621 gtctggggcg cattgatagg agtgatcttg gccgccatga gcagcgggta cgcgttcgga
     1681 ggttgggtct ccgatcgcta taccggcgga ggagtaccgg ccgtgctgtt ggccttttca
     1741 ggaagctgga cgtttctcgt cgcgtgggcg aaccaaccca ttttgtttga gatcgaaaag
     1801 atggtgcaag accctcgctg gggcccttgc ctcgccgcaa ccgtactcct cgcccctccc
     1861 gcgttcggac tcagcggcgt cttgccggcc atgttgcgct tggcagtggc ggacatggag
     1921 cacctcggtc gacagacggg ccgcatgatc gccttatcga ctgtcggcag tctcgcgggc
     1981 acctggggca ctgcattctt tctcttatcg tggctcggaa gccaatcgtt gatggcctgg
     2041 ctgggcggca tgcaggtcgg acttggggtc ctgtggatta tgaagggaac atcggctcgc
     2101 ccattggtat tgctgattgc tctgggttgc ttcgtcctgt tgggagccat ggctctcaac
     2161 ccgatcaaac gactgaagac ccccgtctat caagaggaaa gtccgtacca gcaagtccgt
     2221 attcgcgaag atgatctctt ccggtatctc gtgttggatc gtacgttcca cgccacgatg
     2281 tggaaagtcg acccgacttc cctttttctc ccctacagcc aaatgatggt gtcttcgctg
     2341 gcactggtgt ccgagccgaa gcgcagtctc attctcggcc atggcggcgg ctcgttggcg
     2401 aagtggttgg ccaggcactg gcccgcgttg gagctggata tcgtggaatt cgacccggtc
     2461 gtcgtccgaa tggccgaaga gtattttgat tatcgcccac cggccaatca tcacgttttt
     2521 gtgaaggacg gtcgagcctt tctcaacgca acggatcaca cgtacgatct tatgtggatc
     2581 gatgcgtttg cgcgggacat gatccctttt cacctcacca cggcggagtt ttactcctta
     2641 gtacgaacgc acctgaaccc ggagggaatc gtcgcggtca atctggcgtc atccggcaaa
     2701 gaaggtgatc tcgctcgagc cgccgcggta gtccaaacca tgaaacaggc atttcccgtc
     2761 ctcgccacat tcgccgtcga ggggccgtgg aagacaggaa tgaccccggc gaagaatctg
     2821 atcttctttg gaggccgtct cattgagcac gaatcgccgg acagtctcat ggcgaagatc
     2881 acggacatgg cgatgaatca gcgcctgccg atggaaacga tcgcgctgtt gagtactcgt
     2941 cggaccgagc cttggccacc cggcgtcgtc ttgagcgacg actttgcccc ttatgatctt
     3001 ctccttgggc gagaacgatc gcaattggtg gaatgaggtc ctcgcccaat ggcgaacagt
     3061 aacgaaggga cgacccctgc cctgtcggat gaacaggcgc ggcggttgct ggagacgccg
     3121 gttgaaaaca cgctgaaggg cgtgcgtgat tgggcattct cgccctctcc catgaagccg
     3181 atattgccaa ggtccaggaa tggcttggtc atgcgaatgt ttcgacgatc cgcctctacg
     3241 atcggcgcaa gaccaggccg gaggactccc ggacgtttcg agttcgatat tgaactactt
     3301 catcaagtag ttgaaaagat gcaaaccgct aggctggagt gtgggatgcc atgcctacgg
     3361 tttcccggat aacgatgcgc tagccaactt ggtaagttgg atcgcctgcg cgagtcggat
     3421 agtctgaatc aacattatct ggagaagaaa cgctacgcac ttgtgcttaa cacgatacga
     3481 cccccagata gtaggacccc tcctgtttta ggcaagtacc gttaaggagg agtttgaaat
     3541 aatcttgtcg atttccatcg catccagcac ctctttttcc aagagcgcat ccgccagcgc
     3601 cttcaaggtc accatgtgtt cggtcagaac atgtttggtc ctctcgtagt tttctgtgac
     3661 gaggcgtttg atttctagat caatttccaa cgccattgca tcactgagat cacgcttagt
     3721 tgcgaaggcg cgtcccagaa acatcgattc ctctttctgc ccgaacgtca gcggccccag
     3781 cttttcactc atgccccatt cgcagaccat tttacgtgcg aggtccgttg cacgctccag
     3841 atcgttgccc gcgccggtgg tgacctgttt gaacaccagc tcttccgcca ctctaccacc
     3901 taggagaatc gccagcgtgg cgtacaaaaa ctcttgggaa tgattgtggc ggtcatcggt
     3961 cggcaactgc atggtcacac ctaatgcacg gcctctcgga ataatcgaca ctttgtgaac
     4021 aggatcggta cccggtagaa gtttggccat caaggcatgg cctgcctcgt gataagcagt
     4081 gactcgcttt tcctcgtccg tgagcatcat gctcttgcgc tcgggaccca tcaaaatctt
     4141 gtctttcgcc atttcgaagt ctacgactcc aacttcattt ttgttctgac gggccgccca
     4201 gagcgcagcc tcattgacca agttttccaa gtcggcacct gaaaaaccag gggtccctcg
     4261 cgcaatcttt tcgagctcta catcggctgc tacggggatt ttcttcgtgt gcaccttcag
     4321 gatcgctgaa cggcctcgaa ggtctggatg attcactgtc acttgccggt cgaatcggcc
     4381 tggcctgagc aaggccgggt cgagcacatc cggccgattg gtcgcggcaa ccaggataat
     4441 gccttccgtc gtatcaaatc catccatctc gaccagcaac tggttgag
//
LOCUS       DATMTI010000803         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_751444, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000803 DATMTI010000000
VERSION     DATMTI010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751444"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VN039_12735"
     CDS             complement(<1..895)
                     /locus_tag="VN039_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV46862.1"
                     /translation="MNTVVFVVDDEQVIRTAIVKRLIRQGHFATGYESGEVLLQELEH
                     KLPELVLLDLKMPGISGLDALKHVRQLAPSALVIMLTAYGTVQDAVEAMKLGAYDFLI
                     KTVDLEGVEAVVARAVEILKLRRRLESELDGQTNRYHLSNVEAHSRAMKQLLEQVREV
                     AENPKPTVMLLGETGTGKEFLARVIHHNGPRASGPFVGVNCTAIPKDLFESELFGYER
                     GAFTGANQRKLGLLEKAEGGTLFLDEIGDLDLTMQAKLLRVIQERSFRRLGGTDDLGA
                     DFRLITATNREIKKEVERGAFR"
     gene            complement(899..>2428)
                     /locus_tag="VN039_12740"
     CDS             complement(899..>2428)
                     /locus_tag="VN039_12740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV46863.1"
                     /translation="QAIQQGNRTEAIQYVREGRSREIMREVRNWMASFDQEQQGMQQQ
                     ELSLLSYDRTWTRFLILGGGLVTLCLVILALALIAHSIATPLTALSKVVGSTAGQTIP
                     SIPVFDRKDEIGELTTVMKKMSLQIRKDLDEVQQSEAALKKLNAHLSASEVKYRGIVD
                     HAPLGIFMTKGVRVTFSNRYNQQLAGLDPEAAVDPETFRQRMHPEDSDRVYATFSEAV
                     AAGRPCEIIFRLLQGDGSIRTVLSQRVPIMDLESPDVVYVGFNIDITTLDNLQLQLRR
                     AEKLATLGQVAAGIAHELRNPLVGIGSTAKVLLDDFESGDPKRKEIEVILSETRRLDR
                     IVNQIVDYARPRRPVPIRIDLTQLAGEVSKMLRPRLESKHLTIKVSISPMISEFTADR
                     DLLRQVLLNIVDNALDATPKGSAPIEITGHELFREERPGLVIQVKDQGVGIPQDLLPN
                     VFQPFVTSGKKHGTGLGLAICQNIIESHEGNIYVTSEVGKGTIVGIWLPLEQKTALER
                     V"
BASE COUNT          518 a          714 c          608 g          588 t
ORIGIN      
        1 ctcgaaaagc tcctcgctcc acctccttct tgatttctcg gttcgtcgcc gtgatgaggc
       61 ggaaatcagc tccgagatca tcagttcccc cgagccgccg gaatgatcgt tcttgaatca
      121 ctcgcagcag ctttgcctgc atcgtcagat caagatcccc gatctcgtca agaaacagcg
      181 tgccgccttc cgctttttca agcaaaccaa gctttcgctg attggcaccg gtaaaggccc
      241 cgcgctcata tccgaacaat tcactctcaa agagatcctt gggaatggcg gtgcagttga
      301 cccccacgaa tggacccgac gccctcggcc cattgtgatg gataacccgt gccaggaact
      361 ctttcccggt tcctgtctcg cccaacaaca tcacggtcgg ttttggattc tccgccactt
      421 ctcgtacttg ttcgagcaac tgtttcatcg cacggctgtg agcctcgaca ttactcagat
      481 ggtagcggtt ggtctgtcca tctagttccg attccaagcg gcggcggagc ttcaagatct
      541 cgaccgcacg ggcaacaacc gcctccactc cttcaagatc gaccgttttg atcaagaaat
      601 cgtacgctcc caatttcatg gcttccaccg catcctgcac cgtcccatac gcggtcagca
      661 tgatgacgag ggcggaagga gcgagttgac gcacgtgttt gagggcatca aggccgctga
      721 tgccaggcat ctttagatcg aggagcacca gctcggggag cttgtgctca agctcctgca
      781 acaacacctc gccggattca tagccggtcg cgaaatgtcc ctgtctgatc agccgcttga
      841 cgatggccgt gcggataact tgttcgtcgt cgaccacaaa aacgacggta ttcatggatc
      901 aaaccctttc caacgcagtt ttttgctcca gcggtaacca aatgccgaca atcgttccct
      961 ttccgacttc gctcgtcaca tagatattcc cttcatggct ttcgatgata ttctgacaaa
     1021 tagccaagcc caatcctgtc ccatgttttt tcccggaggt cacgaatggt tgaaacacat
     1081 tcgggagcag gtcctgcggg atacccacac cttgatcttt gacttgaatc accagaccgg
     1141 gcctttcctc tcggaataac tcatgccctg tgatctcgat gggagcgctg cccttcgggg
     1201 tggcatctaa ggcgttatcc acgatattca agagtacttg tcgaaggagg tctcgatcgg
     1261 cggtgaactc gctaatcatg ggtgaaatcg agaccttgat cgtgaggtgc ttactctcca
     1321 atcggggcct caacattttg gacacctcgc cggcgagctg ggtgagatcg attcgtatcg
     1381 gcaccggtcg gcgtggcctt gcatagtcca cgatctgatt gacgattcga tccagtcgtc
     1441 gcgtctccga caagatcacc tcgatctctt tacgcttcgg gtcgccggat tcgaagtcat
     1501 ccagcaagac cttggccgtc gacccgatgc ccaccagcgg attcctgagc tcatgagcga
     1561 tgccggccgc cacttgcccc aaagtcgcca acttttccgc tcgccttaat tgcaattgca
     1621 gattgtcgag agttgtaatg tcgatattaa agcccacata gacgacatcg gggctctcca
     1681 aatccatgat cggcacacgt tgactcaaaa ccgttcggat actgccgtcg ccttgaagta
     1741 agcgaaaaat gatttcgcag ggtcgccctg cggctacggc ttctgaaaat gtcgcataca
     1801 cgcggtcgct atcttcagga tgcatccgtt ggcggaatgt ctcgggatcg acagccgctt
     1861 caggatccag ccccgccagc tgctgattgt aacgattgct gaacgtgact ctgactcctt
     1921 ttgtcataaa gatgccgagc ggcgcgtgat ccacgatgcc tcgatacttc acctcggaag
     1981 cggacaagtg cgcattcagc ttcttgaggg cggcttctga ctgctggact tcatcgagat
     2041 ctttgcggat ctgtaagctc atttttttca tcacggtggt gagttccccg atttcgtctt
     2101 tccggtcaaa gacagggatg gaggggatcg tctggccggc cgtcgaaccc acaacctttg
     2161 ataaggcggt taatggagtt gcgatggaat gggcaatgag cgccagcgcg aggatgacga
     2221 ggcagagcgt caccaacccg cccccaagaa tgagaaagcg tgtccacgtt cgatcgtagc
     2281 tcaacagaga tagttcctgc tgctgcatgc cctgttgctc ctgatcaaac gatgccatcc
     2341 agtttcgaac ctctcgcatg atctctcgac ttcgtccctc ccgaacatac tgaatggcct
     2401 cagttcgatt tccctgctgg atggcttg
//
LOCUS       DATMTI010000804         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_708207, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000804 DATMTI010000000
VERSION     DATMTI010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708207"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1522)
                     /locus_tag="VN039_12745"
     CDS             complement(<1..>1522)
                     /locus_tag="VN039_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV46864.1"
                     /translation="ERLSATYQAATIGIAEADSAGRLLRVNDALCRMLGRSEDELLAM
                     TFLDYTHAEDKKQDGERYAQQVEGKIDSYVLRKQAIKPDGTSVYLDVHSSSVRDAKGE
                     FRYGVRVLIDVTKAERMEDRIRENERNLRDLLEALPAAVYTTDADGRITFFNRAAVEM
                     AGRVPTLGDEWCVTWRVFRTDGTFLPHDQCPMAVAIKEDRPIRGEEAVAERPDGTRVP
                     FIPYPTPLHDSEGRLTGAINMLVDISDRKKAEEFAERLAAIVTFSDDAIVSKDTQGII
                     QTWNKGAERLFGYAAADVIGKPITILIPPERQDEEPEILARICRGEHIEHYETVRMKK
                     DGSLIDVSLTVSPLKDAKGQIIGASKIARDITEKRQEERRHLLLINELNHRVKNTLAT
                     VQSLASQTFRGERDSRAFRQFENRLVALARAHDLLTQESWQGADLLEIVNRALQAVCP
                     DREGQLEVSGPPCRLNPKLALSLSMAFHELATNATKHGALSVDAGKVAVDWNIEGGG"
BASE COUNT          282 a          470 c          417 g          353 t
ORIGIN      
        1 cccgcctccc tcaatattcc agtcaactgc gactttgcct gcatcaaccg acagagcccc
       61 atgtttcgtt gcgttggtgg caagttcgtg aaaggccatc gacagggaga gcgcgagttt
      121 gggattcaac cggcagggcg gtcccgatac ctcaagctgg ccctcccgat cggggcatac
      181 ggcctggagc gccctgttca caatctctag cagatcagca ccctgccagc tttcttgcgt
      241 gagaaggtca tgtgctcggg caagcgcaac aaggcggttc tcaaattgcc ggaaggcgcg
      301 actgtcccgt tcgccacgga acgtctggga agcgagggac tgcaccgttg ccaaggtgtt
      361 tttcaccctg tggttcagct cgttaatgag caacagatgt cgccgctcct cctggcgctt
      421 ctccgttatg tcgcgggcga ttttcgatgc cccgatgatc tgccctttgg catctttcaa
      481 gggagaaacc gtgagcgaga cgtcgatcag gcttccgtct tttttcatgc gcacggtttc
      541 gtaatgctcg atatgctcgc ccctgcagat gcgcgcaagg atttccggct cctcgtcctg
      601 ccgttcggga gggatgagga tcgtaatcgg ttttccgatc acgtccgcgg ccgcataccc
      661 gaaaagtcgt tcagcaccct tgttccacgt ctggattatg ccctgggtgt ccttgctgac
      721 gatcgcgtca tccgagaagg tgacaatcgc ggccagccgc tccgcaaact cttccgcttt
      781 cttccggtcc gaaatgtcaa ccagcatgtt tatggcgccc gtcagcctac cctcgctgtc
      841 atgcaacgga gtagggtagg ggataaacgg aacgcgcgtc ccatccggcc tttcagcaac
      901 cgcctcttct ccacgaattg gccggtcctc tttaatggcg accgccatcg gacactggtc
      961 gtgcggcaga aaggtcccgt ccgtccggaa gacccgccag gtgacgcacc actcatcgcc
     1021 caacgtcgga acgcggccgg ccatttctac cgccgcacgg ttgaagaacg tgatgcgacc
     1081 gtcagcatcg gtagtgtaca ctgcggccgg taatgcctcg agaaggtcac gcaggttcct
     1141 ctcgttctct cgtattcggt cttccatcct ttcggctttg gtgacgtcga tgaggacgcg
     1201 cacgccgtaa cgaaactctc ctttggcgtc ccgtaccgac gagctatgaa cgtcgagata
     1261 cacggacgtg ccatccggtt tgatcgcctg cttgcgcaat acgtagctgt cgattttgcc
     1321 ctcgacttgc tgcgcatagc gctctccgtc ttgctttttg tcttcggcat gcgtgtaatc
     1381 aagaaatgtc atcgcgagga gctcgtcctc agagcgtccg agcatccgac aaagcgcgtc
     1441 gtttacccgc agaaggcgtc cagctgaatc tgcctctgcg ataccgatcg tcgcagcctg
     1501 ataggttgcc gaaagacgtt ct
//
LOCUS       DATMTI010000805         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_581577, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000805 DATMTI010000000
VERSION     DATMTI010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581577"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1204)
                     /locus_tag="VN039_12750"
     CDS             complement(314..1204)
                     /locus_tag="VN039_12750"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWV46865.1"
                     /translation="MSAPAPSVPPLKLTYPVYVTVLLFGVIVGSALIGVPAFGLVYGY
                     TWLDWTMFGLLYIVTGLGITVGYHRLISHRSFTCSDWIKAGLLIAGGCALQNSALKWG
                     ADHIRHHAACDQDADPYNAQRGFWHSHCGWLFADQRYSDEKYATRLRQDPIVMWQHRY
                     YTPTFLSGLALPLVVGFLYGGWIGGFSCFMLAGVGRTFAVLNSTFCINSVCHLWGSQQ
                     HGQADSSRDSWLVSLVTFGEGYHNYHHTYQSDYRNGPRWYNFDPSKWLIFTLSSFGLV
                     WSLRTAHALEPKVSDRSASS"
     gene            complement(1864..2824)
                     /locus_tag="VN039_12755"
     CDS             complement(join(1864..1935,1937..2824))
                     /locus_tag="VN039_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016721870.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWV46866.1"
                     /translation="MHQQTFAEVTFEPYRKPTRRERFLDEMNRVVPWADLVAAIEPVY
                     PTAEGPGRPPVGVERMLRLHCLQQWFNLSDPAVEEALYDSRAMRQFVGIELGREPVPD
                     ETTICKFRRLLETYQLGEQLFARIGEYLAKHGLQVSRGPIVDATIISAPSSTKNRQKE
                     RDPEMHQTKKGNQWYFGMKAHIGVDSQTTLIHSVAATAANVHDSQMLPQLLHGQETRV
                     WGDAAYSGQRDVIRQHAPHAKSFIQTKAHRHRPLSETERARNRTKSKVRAKVEHAFLV
                     IKRIFGWATVRYRGLAKNTPWLFISCGLANLYVARRRLLAGAS"
BASE COUNT          644 a          811 c          799 g          604 t
ORIGIN      
        1 cctgcaatga atgacggtac gtggttggtg aataaatgtg tttgcgtagg gtattgaatc
       61 gtctctcttc aacgtgtcta tagatttgaa gtgcaactga ttaaaggcga gggtgtaatc
      121 ggaacggaat ctctagcgca caccaacgat gcggtcccca tcggaaggag agagcccctt
      181 ccagtcaccg agccacatca gtcatgtagt gtcgccctgt gaaaatctac ctaacgcgcg
      241 gcaatggatt gggggactcg ggctgacaga ctctttcggc gaatcccagt tactcctaaa
      301 tggtctccgg ctactatgaa gaggccgaac gatctgaaac ttttggctca agagcatgag
      361 cagtacggag ggaccagacc agtccgaatg atgaaagcgt gaagatcagc cattttgagg
      421 gatcgaagtt ataccagcga ggaccatttc ggtagtcact ttgatatgtg tggtggtagt
      481 tgtgataccc ctcgccgaac gtgactagcg agaccagcca gctatcccga ctcgaatccg
      541 cctgaccatg ctgttggctt ccccagagat gacacacgga attgatgcaa aaggtcgagt
      601 tcagcacggc aaaggtccga ccgactccgg ccagcataaa acagctaaag ccgccgatcc
      661 aaccgccata gagaaaaccc acgacgagcg gaagtgccaa acctgagagg aaggtcggtg
      721 tgtaataacg atgctgccac atgacgatcg gatcttggcg gagccgcgtc gcatacttct
      781 catctgagta gcgctggtcg gcgaagagcc atccgcagtg gctgtgccag aatcctcgct
      841 gcgcgttata gggatcggca tcttggtcgc aggcagcatg atggcggatg tgatcggccc
      901 cccacttcag ggcagagttt tgtagtgcac atcccccggc gatcagtaat ccggccttga
      961 tccaatcgga acaggtgaaa ctcctgtgtg aaatcaggcg atggtagccg accgtaatgc
     1021 cgagtccggt gacgatatac agcaatccga acatggtcca gtctaaccac gtgtacccat
     1081 agacaaggcc gaaagcggga acacctatga gtgcgctccc cacaataaca ccgaacaata
     1141 gtaccgtaac gtaaacagga tacgtaagct tgagaggggg aaccgatggt gcgggtgcgc
     1201 tcataatggc gatatgcgag ggacagcgtt catgggaagc agagactcta acccaggctc
     1261 agccagccgg atagtggatt gagggaggcc tatgtagtca tgcatgaatg aggataatac
     1321 ggtttcgtta cgcttgttac tgggaaaacg cttagtagga gggccagact gtattctgga
     1381 ctccagactc ctggcgagaa agcttgaaat ccgtcctttt gtcccggtgg cgcaaagcca
     1441 ttatgggagc cccaagcttg tgccgtggca gggtaatgcc agtcatggct ggtaaatcca
     1501 acgcattggt gatgctgacc atactgctac ctttaactga tacaatatga gcatgcctcc
     1561 gaaaaataac ctcaggtcag gttcgcccgt caaatacaga gggaagcgct tcaagaggcg
     1621 actttgtctc aagtccacga gcaaactact tccacccgat accagccgta tcccgtggtt
     1681 tccacctctt cccaagactg gaaccgatgc ctagggaagg cctgattaat tcacgtttgc
     1741 acgaagattt ctgtttctga gtgggtcaac acgggccatc agctgggtat ccgctggtcc
     1801 gggagcatcg ttcttgcggt tcgcccgcca tcgggccgcc catgcccccc ttcacacgca
     1861 tgctcacgag gctcccgcca gtagccgccg tcgcgccacg tacaaattcg ccaagccaca
     1921 actgatgaac agccagtggg gtgttcttcg ccagcccacg gtaccggact gtggcccacc
     1981 cgaagatccg cttgatcacc aagaacgcat gctcgacttt cgcacggact ttcgacttcg
     2041 tgcggttccg ggcccgctcg gtctcactca gcggccgatg ccgatgggct ttggtctgaa
     2101 tgaagctctt cgcatgaggc gcgtgttgcc ggatcacatc gcgttgcccg ctataggccg
     2161 catcccccca cacccgggtc tcctggccat gcaggagctg gggcaacatc tggctgtcat
     2221 gcacattggc cgccgtggcc gccaccgaat ggatcagtgt ggtctggctg tccacgccga
     2281 tatgcgcctt catgccgaaa taccactggt tgcctttctt cgtctgatgc atctccggat
     2341 cccgctcctt ctggcgattc ttcgtcgaac tgggtgcact gataatcgtg gcatccacaa
     2401 tggggcctcg gctgacctgt aagccgtgtt tcgccagata ctccccaatc cgtgcaaaga
     2461 gctgttcgcc cagctggtag gtttccagca gacgccgaaa cttgcagatc gtcgtctcat
     2521 cgggcacggg ctcgcggccc agctcaatgc ctacgaactg ccgcatggcc cgtgagtcgt
     2581 acagcgcttc ctcgaccgcc ggatcggaga gattgaacca ctgttggaga caatggagcc
     2641 gcagcatgcg ttcgacgccg accggtggac gtcccgggcc ttcggccgtg ggatagaccg
     2701 gctcgatggc cgccaccagg tccgcccacg gcacgacgcg attcatttca tcgaggaacc
     2761 gttcccggcg agtaggcttg cgatacggtt caaacgtgac ctcggcaaat gtctgttggt
     2821 gcatggatgc gcctcccgtt cagtgctgcg ctacccgt
//
LOCUS       DATMTI010000806         1726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_751496, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000806 DATMTI010000000
VERSION     DATMTI010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751496"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..>1726)
                     /locus_tag="VN039_12760"
     CDS             complement(218..>1726)
                     /locus_tag="VN039_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46867.1"
                     /translation="QALPDGTPLSHIVESLTVIYGVGWNVWTAALRADAKSSNDSLAN
                     ATLPATPIIPSLVASSPNGRALGQNTPPAMNADGTVSVGGAYDGIVTLNSGISVQFTR
                     PTDPNRFDAQRSFEQGIGSIMGLLYSLTDFMPIDLFSWAAPNSRNFDVSGRRYFSIDG
                     GVTTIIDYNQSPNFDFASWLSPQCPQAHPFVQNSLPCAGQSADISATSPEGINLDVIG
                     YDITQPQSGPPRLGNLSTRSFVQTGDNVMIGGFIVQGTGPKRVIIRAIGPELAPFGVP
                     NPLANPTLELHDGTGSRIASNNDWQTTIIGGIITGNQVTDIQNSGHAPASAGESAIIA
                     DLQPGNYTAILSGVNGTTGVALVEVYDLAPDATASLGNVSTRSFVQTADNVMIGGFIV
                     QGTGPKRVIVRAIGPELSQFGVPNPLANPILELHDGTGAVVGTNDDWQTTIIGGIITA
                     SQVTDIQNSGHAPTAPSESAIIATLQPGNYTAIVRGVNNTTGVALVEVYDLH"
BASE COUNT          391 a          477 c          469 g          389 t
ORIGIN      
        1 cgcgcggcat tcgtgtaagc acgaatcttg aagggatttt cgtccttcag ttccagcagc
       61 gtggcgattt gctccagcac gccggcgatt tcctctttgg tcatcaccag aacgtgaacc
      121 ggaatcaggt cgccgtcgac ggcgaagcgc tgcgccgcaa gttcacttgc atctgacgta
      181 cgacgttatc gcacgcacaa gccaaacccg atttctgcta atgcagatcg taaacttcca
      241 ccagcgcgac tcccgtcgtg ttgtttacac cacggacaat cgcggtgtag ttgcctggct
      301 ggagggttgc aatgattgcc gactcactgg gtgctgtagg agcatggcca ctgttctgaa
      361 tatcggtgac ctggctggcg gtgatgattc cgccgatgat tgtcgtctgc cagtcatcat
      421 tggtgccaac gactgctcca gtcccgtcgt gcagttccaa tatcggatta gccaacggat
      481 tcgggacccc aaattgtgag agctcagggc caatggcacg aacgatgact ctcttcggcc
      541 ccgtgccttg aacaatgaaa ccgccaatca tcacgttatc ggccgtttga acaaagctgc
      601 gagtactcac gttaccaagg ctcgcagtcg cgtcgggagc caaatcgtat acctcgacca
      661 gagcaactcc ggtggtgcca tttacgccac tcaggatcgc ggtgtaattg cctggttgta
      721 aatccgcaat gatcgccgac tcacccgccg aagcgggagc atgaccgctg ttctgaatat
      781 ccgtaacctg attgccggta ataattccgc cgatgatcgt cgtctgccaa tcgttgttac
      841 tagcaatccg cgatccagtc ccgtcatgca gttccaatgt cggattagct aatggattgg
      901 ggaccccaaa tggcgcaagc tcagggccaa tggcacgaat gatgactcgc ttcggccctg
      961 tgccttgaac aatgaaaccg ccaatcatca cgttgtcgcc cgtttgaaca aagctgcggg
     1021 tactgaggtt acccaacctt ggcggaccgc tctgaggttg agttatatcg tagccgataa
     1081 catccagatt gatgccttca ggcgatgtgg cagagatgtc agcagactgt cccgcacacg
     1141 gcaacgagtt ttgcacgaag ggatgcgctt gaggacactg ggggcttagc cagctggcga
     1201 aatcgaagtt tgggctctga ttgtaatcaa tgatcgtagt aactccgcca tcgatcgaaa
     1261 aatagcggcg ccctgaaaca tcgaagttcc tgctgttagg cgctgcccaa ctgaacagat
     1321 cgataggcat gaaatcagta agactgtaga ggaggcccat gatgctgcca attccctgct
     1381 caaacgaacg ttgcgcatca aacctgttgg ggtcggtagg gcgagtgaac tgaaccgaga
     1441 ttcccgaatt cagcgtgact atcccatcat atgcgccacc gacactgact gtgccgtctg
     1501 cgttcatcgc cggtggagtg ttctgcccca gggcccgacc atttggacta gacgcgacaa
     1561 ggctgggtat tattggggtc gccggcagag ttgcgttcgc aaggctgtca ttgctcgatt
     1621 tcgcatcagc tctcaaagca gcagtccaaa cgttccagcc aacaccgtag atgaccgtga
     1681 gactctccac gatgtgactc aggggagtgc cgtcagggag agcctg
//
LOCUS       DATMTI010000807         6500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_284445, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000807 DATMTI010000000
VERSION     DATMTI010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284445"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1467
                     /locus_tag="VN039_12765"
     CDS             301..1467
                     /locus_tag="VN039_12765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWV46868.1"
                     /translation="MAYDTKTLLSVIIGLGVGLFYLDLYLPSGISTGVLYGGLVILSF
                     LLPYRKGPLIAAAVCSVLDLAGTDLGLTIAGVPHWMSVVNRLFSLTALWLPLLFFLHR
                     RKAEDELRQAHDELEARVHARTQQLATLNQSLITEIADRKETEQSLRASEAALQARER
                     QLQQNREELRALAGQLLTAQEEDRRRISRDLHDHINQRLAMLTLDLRQMQKGPLTDFD
                     HLRDEIRRVSECITLISDDVRQMAYRFHPSILDDLGLVKAVRSLVDDFSTHTGIRSSY
                     MHNDPVAALPDEVTIGIYRIVQESLSNVARHAQASHVEVEVMCDEEMIDLSIRDNGVG
                     FDLGQSSKPGGHLGLLSMKERVRLAQGTLEVESIPGHGTHIRVHIPLIQGGRHG"
     gene            1460..2104
                     /locus_tag="VN039_12770"
     CDS             1460..2104
                     /locus_tag="VN039_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV46869.1"
                     /translation="MAKPRVLLADDHLLVLEGFRRILEGQYELVGTVEDGRALLEAAK
                     RLQPDIVILDVSMPLLNGIDAAAQLKKICPGTKIIIVTMHADKEYIRSAFEAGASAYV
                     LKRSAVDELDQAIRAAVAGHSYITPLITNDMLNVFLSTASETSGGTHHLTMRQREVLQ
                     LLAEGRTAKEIANTLNISSRTVEFHKSQILAQLNLQTTADLIKYALTHGIMSAS"
     gene            2369..5548
                     /locus_tag="VN039_12775"
     CDS             2369..5548
                     /locus_tag="VN039_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV46870.1"
                     /translation="MNQLVLIALRRPYTFVVMSILIVFLGSKTIRHMPTDVFPNITIP
                     VTSVVWIYSGMIPPQVEGRITYMFERFLTSTVEGIKYIHSHSYYGSSITNIFLQDGID
                     VGRAEADIVGIAQNVVKALPPDISPPMVMRLAPSSIPVAMLEISSEDMTPAELYNLTY
                     MRIRPLMVTVPGIVLPHPYGGQDMQVMVNLDPHKMLARHLTPSDIHDVLMKQYLVLPS
                     GDIKIKQTDWVVLTNASPLKIDDFSNIPIKREGNAYVYLRDVASVRLMGRVQQNMVLV
                     KGKQTVILVAMKSTEASTLDVVEGIKKMIPRAERVSPEGVRIRLLDDASTFVKDSISD
                     VLHEMLTAGALVGLIVLLLLGSWRATVIVWVSIPLSILTAIIGLHWLEETINVMTLGG
                     LALAVGILVDDATVMIENIDRHLEMGKPLEQAIIDAANQIVVPTLVATLCIAIAWFPL
                     FDLSGVAGYLFKPMAEAVMIAMIASFIFSRTLVPTMAKYMMKSHHDQPHEHQPHGQPA
                     AEPSRNVFVRFQQGFEHRFNRFRDSYEALLKRMIARRRAFVTVSLAVAVGSMGLFFFL
                     GRDYFPEIRSGIIQMHMRAPLGTRIEVSGRITTLVATSIEELLPDQVENIVSNCGLPV
                     GPHNLAFIPTPTIGSQDCDMTILLHDEKSPVWDIRKTLRKGLKERYPGTEFTFQPSDL
                     TAKILNFGAPAPIDVQVNGPDTYPSYEFTLKLADEFRRIPGAKDVVVHQTMRTPTMMV
                     EGNRTFGLNVNRTERDMAENLLMTTAGSQQIDQIYWLDPSTGMSYLINVYTPQPFINS
                     VNSLKTVPVDSSNDLKGEVQLLGNLSDLIPEGTPGVITHGNIMPLFDIYVSVEDRDLG
                     GVLADVQKITKSMEDQKPRSAEVEIHGQASLMYEAYAELIFGLLVAIMLIYLLIVVNF
                     QSWLDPFIIITALPGALAGIAWALFLTHTRLSEPALTGAIMCMGTATANSILVVSYAR
                     EMLQEHGDALRAAIEAGTTRFRPVLMTAAAMIMGMVPMATGYSQNAPLGRAVIGGLLM
                     ATLFTLLFVPCVYAIIYSRRMAQQPKEIS"
     gene            5545..>6500
                     /locus_tag="VN039_12780"
     CDS             5545..>6500
                     /locus_tag="VN039_12780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV46871.1"
                     /translation="MIATLVRSRIAIAAVILCGLYLGYRIHEAQSEAASLRDRTLIEA
                     VPTVSVVAPKPTPKSETIVLPGNIVGWYEAPIYARVTGYVKMWYKRYGDEVKKGDLLA
                     EISTPDLDAEYRQAQADLESERAKYKLADMTKNRWVALRPNHAVSEQSITVQEQDWKA
                     QAALVKAAEQKVKNIEAFIGFKRIIAPFDGIVIQRNINVGDLVSKEGNLSTPNVKSNL
                     FTVAVVDKLRLFVNVPQTFGPFLRPGLTADVTVAPLPHRHFNFEFLTVAKGFDIGTRT
                     ATTIFTIDNEDHALWPGTYAQVHMTAPIDRPAFILPSSVLIY"
BASE COUNT         1421 a         1907 c         1757 g         1415 t
ORIGIN      
        1 aggccttgct tttcaccggc atccacagac ggtgggtagg tgtctcgggg atcctggtaa
       61 cttatcgagt ggggccctgt tgatttccac agtgctaatt tgaaggactc tactcaaatt
      121 gtttcggcag catggcgatc aggcgagtcg cgcaaaccaa ggaagctcgt ggcataatac
      181 ttccaaccga tcaaccaaca ggtgcgaggc gttccacgag ccgataggga tgttcacagt
      241 gttaaggatt gttacataga ttctagagtt tcattttttc attccattaa gactcacatc
      301 atggcctatg acaccaaaac cttgctgtcc gtcatcatcg gcctcggtgt tggtctgttc
      361 taccttgacc tataccttcc aagcggcatc agtaccggag tactctatgg cggactggtc
      421 atcctctcat ttctactgcc gtatcgaaaa ggtccactca ttgccgcggc ggtctgttcc
      481 gtactcgact tggccggtac cgacctcggc ttgaccattg cgggcgtacc ccattggatg
      541 agcgtagtca atcgtctgtt cagcctcacg gctctctggc tcccgttgtt gttttttttg
      601 catcgccgca aagccgagga cgaactccgg caagcgcacg acgaactcga ggcgcgagtg
      661 cacgcgcgga cacaacaatt ggcgactctc aaccaatcct tgatcaccga gattgctgac
      721 cgtaaagaaa cggaacagtc gctgcgcgcc agcgaagccg cgctgcaggc cagggaacgg
      781 caacttcagc aaaatcgaga agagttgcga gcgctggccg ggcagcttct gacggcgcag
      841 gaggaagatc gtcgaaggat ctcccgcgac ttgcacgacc acatcaatca acggctggcg
      901 atgctgacgc tggatcttcg acagatgcag aaaggcccat tgaccgattt cgatcatcta
      961 cgggatgaga ttcgcagggt gtccgaatgc ataaccctca tttctgacga tgtacgccaa
     1021 atggcctacc gctttcatcc atctattttg gatgatttgg gactggtgaa agccgtgcgc
     1081 agccttgtcg atgacttttc aacccacacg ggtatccgga gcagctatat gcacaatgat
     1141 cctgtagcgg cactgccgga cgaagtcacc atcggcatct atcggatcgt gcaggaaagc
     1201 ctcagcaacg tcgctcggca tgcccaggcc tcacatgtcg aagtggaggt gatgtgcgac
     1261 gaagagatga tcgacctgtc cattcgcgac aatggagtgg ggttcgatct cgggcagtca
     1321 agcaaacccg gcgggcacct gggattgctg agcatgaagg agcgtgtgcg gttggcgcag
     1381 ggcacgttgg aggtggaatc gataccagga cacggcaccc atatccgagt gcatatcccg
     1441 ctgattcaag gaggacggca tggctaaacc gcgcgttctg ttggccgacg atcatctcct
     1501 ggtgttggag ggattccgcc ggattctcga aggacagtat gaattggtcg gtaccgtcga
     1561 ggacgggcgt gcgctcttgg aggccgcaaa gaggcttcag cctgatattg tcattcttga
     1621 cgtatccatg ccattgctga acgggatcga cgcggcggct caactgaaga agatctgccc
     1681 cggcaccaaa atcatcatcg tcaccatgca tgccgacaag gaatatatcc gatccgcctt
     1741 tgaggcgggg gcatcggcct atgtgctcaa gcggtcggcg gtagatgaac tagaccaagc
     1801 cattcgggcg gcagttgcgg gacattccta cattacccca ttgattacca acgacatgct
     1861 caatgtcttt ctctcaacgg catcggaaac atcgggtggg acacaccacc tcaccatgcg
     1921 tcagcgagaa gttctgcagc tgctggccga gggacggact gcaaaggaaa ttgcgaatac
     1981 tttgaacatc tcttcacgga cggttgagtt ccataagagc cagattttgg cgcagctcaa
     2041 tctgcagacc accgcggatt tgatcaaata cgctctgacc cacggaatca tgtccgcatc
     2101 gtagcatatc taccttagtg tcatttactt ttccatcgac ctcgtaatat tttccgtacc
     2161 ttcttcggaa tttcccagtt cctcccgcgg ggtctgtgcg ttataacgcc atacaggtga
     2221 aacatcgaga acttcttgta tcgggaggac acatgttggc cggcaaaaga cacttccgtg
     2281 ctggggacct cgtaaaggat gagccgcaat ttctcccaga gcaatttctc ccagagatag
     2341 acaggatgaa gcagaggagg cagaccgtat gaatcaactc gtcctcattg ctctccgccg
     2401 gccctacacc ttcgtcgtca tgtccattct catcgtgttc ctcgggagca agacgatacg
     2461 ccacatgccg accgacgtct tcccgaatat cacgattccc gtcacctccg tggtctggat
     2521 ctattccggc atgatcccgc cgcaggtgga agggcgcatc acctatatgt tcgaacgctt
     2581 tttgacctcg acggtcgagg gcatcaagta tatccatagc cattcctatt acggcagcag
     2641 catcaccaac atctttctcc aggacggaat cgacgtgggc cgggccgaag cggatattgt
     2701 gggcatcgcg caaaatgtcg tcaaggctct gccacccgat atttctccgc ccatggtcat
     2761 gcgtctcgcg ccgtcttcta tcccggtggc catgctcgaa atcagctccg aggatatgac
     2821 gcccgcagag ctctataatc tcacctacat gcgaatccgt cccctgatgg tcacggtacc
     2881 ggggatcgtc ctgccgcacc catacggagg gcaggacatg caggtcatgg tcaatcttga
     2941 tccgcataaa atgctcgctc gtcacctcac gccctccgat atccacgatg tcctgatgaa
     3001 gcaatatctc gtgttgccgt ccggcgatat caaaatcaag cagaccgact gggtcgtcct
     3061 gacgaacgcg tcaccgctga agatcgatga tttttcgaat attccgatca aacgggaggg
     3121 caacgcatac gtctacctac gcgacgtggc gtcggtacgc ctcatgggcc gggtccaaca
     3181 gaacatggtg ctcgtgaaag gcaagcaaac cgtcatcctc gtcgcgatga agagtacgga
     3241 ggcctcgact ctcgacgtgg tcgaggggat caaaaagatg atcccacgcg ccgaacgagt
     3301 ctctccggag ggcgtgagga ttcggctcct cgacgacgcc tcgaccttcg tgaaagattc
     3361 gatctccgac gtgctccacg aaatgctgac cgccggcgcg ctggtcggcc tcatcgtgct
     3421 gctgctgctc ggctcctggc gggccacggt cattgtctgg gtctcgatcc cgctgtccat
     3481 cctgaccgcc atcatcggcc tgcattggct cgaggagacg atcaacgtca tgaccttggg
     3541 cgggttggcg ctggccgtcg gcattctggt cgatgatgcg accgtgatga tcgagaacat
     3601 cgatcgccat ctcgagatgg gcaagccgtt ggaacaggcc attatcgacg ccgccaatca
     3661 gatcgtcgtg ccgacgctcg tcgccaccct ctgcatcgcg atcgcctggt tcccgctctt
     3721 cgatctcagc ggcgtcgccg gctatctgtt caagcccatg gcggaggccg tcatgatcgc
     3781 gatgatcgcc tcctttatct tctcgcgcac actggtgccg accatggcga aatacatgat
     3841 gaagagtcac catgatcaac cacatgaaca ccaaccccac ggacagcctg ccgcagagcc
     3901 gtcacggaat gtcttcgtcc gttttcagca ggggttcgaa caccgcttca atcgattccg
     3961 cgacagctac gaggcgctgc tcaaacggat gatcgcacgt cgccgggcct tcgtcaccgt
     4021 ttcgctcgcc gtcgcggtcg gttcgatggg cttgtttttc ttcctcggtc gcgactattt
     4081 cccggagatc cggtcgggaa tcattcagat gcatatgcgg gcgccgctcg ggacgcgcat
     4141 cgaggtgtca ggacgcatta ccacgctcgt cgccaccagc atcgaagagt tgttgcccga
     4201 tcaagtcgaa aacatcgtca gtaactgcgg cctgccggtc gggccgcaca atcttgcgtt
     4261 cattccgacg ccgacgatcg gctctcagga ttgtgacatg acgattctcc tacatgatga
     4321 aaaatcgccg gtgtgggata tccggaagac gctccgcaaa ggcttgaaag aacgctatcc
     4381 gggaaccgaa ttcacgtttc agccgtccga tctaaccgcc aagattctca acttcggcgc
     4441 gcccgcgccg atcgacgtgc aggtcaatgg cccggacacg taccccagct atgagttcac
     4501 cctcaaattg gccgacgagt ttcgccgaat ccccggcgcg aaggatgttg tggttcacca
     4561 aacgatgcgc acgccgacca tgatggtcga aggcaaccgt acgttcggac tcaacgtcaa
     4621 cagaactgag agagacatgg ccgaaaatct cctcatgacg accgccggta gccaacaaat
     4681 cgatcagatc tattggctcg atcccagtac cggcatgtcg tacctgatca atgtctatac
     4741 gccgcagccg ttcatcaata gtgtcaatag tctcaagacc gttccggtcg attcctcaaa
     4801 cgacctgaag ggtgaagtcc agttgctcgg caatttgtcc gacctgatcc cagagggaac
     4861 gccgggcgtg atcacgcatg gcaacatcat gcccctgttc gatatctatg tttccgttga
     4921 agatcgcgac ctcggcgggg tgctggcgga tgttcagaag attaccaaaa gcatggagga
     4981 ccagaagccc cgcagcgcgg aggtcgaaat ccatggccag gcctcgttga tgtacgaggc
     5041 gtatgccgag ctgattttcg ggctgctcgt cgcgatcatg ctgatctatc tgttgatcgt
     5101 cgtcaatttc caatcctggc tcgatccctt catcatcatt accgccttgc ccggcgcgct
     5161 ggcgggcatt gcctgggcct tgttcctgac ccatacgagg ctgtcggaac ccgcgctgac
     5221 gggcgccatc atgtgcatgg gcacggcgac cgccaattca atcctcgtcg tgtcctacgc
     5281 gcgcgagatg cttcaagaac acggcgacgc attacgggca gcgatcgaag ccggcacaac
     5341 ccgatttcgc ccggtgctca tgaccgctgc ggccatgatt atgggaatgg tccccatggc
     5401 cacaggctat tcgcagaacg cgccgctggg tcgcgccgtc atcggcggct tgctcatggc
     5461 caccctcttc actctgcttt tcgtaccctg cgtgtatgcc atcatttaca gccggcgaat
     5521 ggctcagcaa ccgaaggaga tctcatgatc gcaacattgg ttcgatcacg tatcgccatt
     5581 gcagctgtca tcctgtgcgg actctatctt ggctatcgga tacatgaagc ccaaagcgag
     5641 gccgcttcgc tacgcgacag aactctcata gaggccgtgc ccaccgtctc ggtcgtcgct
     5701 cccaaaccga ctccgaagtc cgagaccatt gtgcttcccg gcaatatcgt cggctggtac
     5761 gaagcgccga tctacgcgag ggtcacgggc tatgtgaaga tgtggtataa gcgctacgga
     5821 gacgaagtga aaaagggcga cctcctcgcc gaaatcagca cgccggatct cgacgccgaa
     5881 tatcgacagg ctcaggcgga tttggaatcc gaacgcgcca agtacaaact cgctgatatg
     5941 accaagaatc gctgggtcgc gctgcgcccc aaccatgcag tgtccgagca gtcgattacg
     6001 gtacaggaac aagactggaa ggcccaggca gcgctggtca aggcagcgga gcaaaaggtc
     6061 aagaacatcg aagcgttcat cgggtttaaa aggatcatcg cgccgtttga cgggatcgtc
     6121 atccagcgca acatcaacgt cggcgacttg gtcagtaaag agggcaatct cagtacccct
     6181 aacgtgaaaa gtaacctgtt tacggtggcc gtggtcgata agttgcgtct ctttgtcaat
     6241 gtcccccaga cgttcgggcc gtttctccgt ccaggcttga cggccgacgt gactgtggcg
     6301 ccgttgcctc accggcattt caacttcgag tttctgacgg tcgccaaggg attcgatatc
     6361 ggcacgcgca ccgcgaccac tattttcacg atcgacaacg aggatcacgc cctctggccc
     6421 ggcacgtatg ctcaagtcca catgacggcg ccgatcgatc gaccggcctt catactgccg
     6481 tcctctgtgt tgatatataa
//
LOCUS       DATMTI010000808         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_454798, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000808 DATMTI010000000
VERSION     DATMTI010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454798"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1178
                     /locus_tag="VN039_12785"
     CDS             240..1178
                     /locus_tag="VN039_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HWV46872.1"
                     /translation="MNIVVSGGTGFVGRALCTALFHGGHRVSILTRNAGQDFHWPGMK
                     ANPVSWNGQDTGPWEQVLEEADAVINLAGAPIADAPWTDVRKQLITDSRVLTTRLLVR
                     TLSRCSSKPGTFISASGIGFYGASDDRRLDEGTARGQGFLADLCLAWEAEALRAAEFG
                     TRVVLLRTGMVLERDGGALPKMLLPFRLFAGGPIMPGNQWISWIHRRDHIGLIQWALT
                     TATVFGPINAVAPEPVMMKTFCEILGRVVHRPSWLPVPEFALNLLLGELGTLMTTGQR
                     VIPAKATAGGYTFQYPTLEPALKAILIGTTIPQQAA"
     gene            1246..>1599
                     /locus_tag="VN039_12790"
     CDS             1246..>1599
                     /locus_tag="VN039_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HWV46873.1"
                     /translation="MTASPIHLGISRCLLGDEVRFDGEHKRDHFLTDILSRYVDWIPV
                     CPEVEAGLGTPREAMRLVGKTRRPRLMTITSKHDHTEAMETMIKKRLTSLDKLDLSGF
                     VFKKGSPSCGVERVPV"
BASE COUNT          354 a          435 c          465 g          345 t
ORIGIN      
        1 attcttgaac atccagccca catcctattg gacggtggtc ccggaagcga accagccatc
       61 gtatgcgcga tacgtagacg cattccttgg taacgtactg agcttcatca aaatatacac
      121 gtatccggtc tggtgcgttc gtgggccgat caagcaggac gatcaataag ttgcgctggg
      181 cgcggtatgg agattccgct tgattgacta gccttgccgc cggctcagga aagaggccta
      241 tgaacattgt ggtatccgga ggaacggggt tcgttggccg ggcattgtgt acagcgttat
      301 tccacggagg ccacagagtc agcatcctca cgagaaatgc gggacaggat ttccattggc
      361 ccgggatgaa ggcaaacccg gtctcgtgga atgggcaaga taccggacct tgggagcagg
      421 ttcttgaaga ggcagatgcg gtcattaacc tcgccggcgc ccctattgcc gacgcaccct
      481 ggacagacgt acgaaaacaa ctcatcaccg atagccgtgt actcaccacc cgcttactgg
      541 tcaggacgct gtctcgctgt tcttccaaac ccggaacctt catcagcgct tccggaatcg
      601 gcttttatgg cgccagcgac gatcgtcgtc tggatgaagg aacagcgcgc ggtcagggct
      661 tcttggccga tctgtgtctc gcatgggaag cggaggccct ccgggctgca gaatttggta
      721 cgcgtgtggt cctcttacga accggaatgg tgctcgaacg agatggtgga gccttaccca
      781 agatgctgtt gccctttcgg ttatttgcag gaggtccgat catgccggga aaccaatgga
      841 tctcctggat ccaccggcgt gaccacatcg gtctgatcca atgggcgctc acgacggcaa
      901 cggttttcgg tccaatcaat gccgtggccc ccgagccagt gatgatgaaa acgttctgtg
      961 agatccttgg gcgggtggtt cacagaccat catggctccc cgttccagag tttgccctga
     1021 accttctcct tggtgagttg gggacattaa tgacaaccgg ccagcgcgtg attcctgcga
     1081 aagcgacagc gggaggttat acatttcagt atccgacact ggagcccgcg ctaaaagcta
     1141 tcctgatcgg aacgaccatt ccccaacagg cagcatgagt tgcggaataa cgacgaatct
     1201 ccttagcaag attgtctcag gtgggctcat ggtgcaggtg acggaatgac ggcttctcca
     1261 atccacttgg gtatcagcag gtgtctcctc ggcgatgaag ttcgcttcga cggtgaacac
     1321 aagcgcgacc attttttaac cgacatactg agccgctacg ttgactggat accggtctgc
     1381 cctgaggttg aagcgggact gggcaccccg cgtgaagcca tgcggttggt aggtaaaacg
     1441 cggcgtcctc ggctgatgac gatcacgagc aaacacgatc acaccgaagc catggagacg
     1501 atgatcaaga agcgtctcac ctcgctcgac aaactggacc tctcgggatt cgtcttcaag
     1561 aagggatcgc ccagttgcgg ggtcgaacgg gtgcctgtg
//
LOCUS       DATMTI010000809         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_284486, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000809 DATMTI010000000
VERSION     DATMTI010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284486"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VN039_12795"
     CDS             <1..541
                     /locus_tag="VN039_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46874.1"
                     /translation="HAEALIRLWTHNASLSAQVAPIGSILIFAAALHCPLFLQYALQL
                     AFAKARLALLVNGILLAIYVPLSILLTLAYAEIGAASAWVALQCIFLGFGTYFTHRRI
                     LTGYAAHWLLCDVGVPFCASLTAAAIVSRIEPQDVLGFEGLIWTGATIVLAVVLSLTF
                     SPGLRTSLREVLLMKKNQA"
     gene            697..>1576
                     /locus_tag="VN039_12800"
     CDS             697..>1576
                     /locus_tag="VN039_12800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWV46875.1"
                     /translation="MSKNEGDGIVEEQNICDALRTLQVRYGRRIAVLLDRRSGIRFNH
                     DLMTVNDEASLGQLVESRVKDGAPSVRLSICMATFNRASFIGMTLDSILDQMQPGVEL
                     IVVDGCSPDGTPEVMADYAGRNPAIRYYRETVNSGVDGDYEKAVAYARGDYCWLMTDD
                     DLLKPGAIRVVLNAIEHHPDLVVLNSEVCNADFSALLTPRFIQLKQDKSYRSDAVDVL
                     FTETAGALSFIGCVVIRRETWMTRMREAYLGTLFIHMGVIFQTPALDQVRVVAEPQIS
                     IRYGNAMWTSRGFEIWM"
BASE COUNT          338 a          392 c          447 g          399 t
ORIGIN      
        1 acacgctgaa gcactgatac gcttgtggac acacaatgca tcactgtcgg ctcaggtggc
       61 accaatcggt tcgatcctga tatttgctgc cgccctgcat tgcccgctat tcctccagta
      121 cgcgttgcaa ctggcattcg ccaaggctcg gctggcgttg ttggtgaacg gaatacttct
      181 cgcgatctat gtgccactgt cgattttact gacacttgca tacgccgaaa taggcgcagc
      241 cagcgcatgg gtggctctgc aatgcatctt tctaggattt gggacatatt ttacgcatcg
      301 tcggatactc acggggtatg ccgctcattg gctgctgtgc gacgtaggtg tcccgttttg
      361 tgcatccctt acggcggctg ccattgtcag ccggatcgag ccacaggacg tgcttggctt
      421 cgaggggtta atctggacgg gtgctaccat cgtgctggcg gtggtgctat cgctgacatt
      481 ttcccctggc ttgcgcactt cccttcgcga agttctgctg atgaagaaga accaggcttg
      541 aaccatcggg agctctgtaa tgtactaaaa aaccgtcagc tacgttgtca aaaatcgtgg
      601 tagtcaacag gtaaatatct atttggcggt gatcggcgct actgtgcata cgcatgatat
      661 ttgtccgaca atttctatga ggcagaaccc tcaagcttga gtaagaatga aggagatgga
      721 attgtcgaag agcagaatat ttgcgatgcg ctgcgaactc tacaagttcg atatggccgg
      781 cgaatcgcag ttctccttga tcgccgatca ggcattcgtt tcaatcatga tctcatgaca
      841 gtcaatgacg aagcatcctt gggtcagctt gtcgaatcac gtgtgaaaga cggcgcgcct
      901 tcggtgcgat tgtccatctg catggcgact ttcaatcggg cgagcttcat cggaatgaca
      961 ctcgactcga tcctcgatca aatgcagcca ggtgtcgagt tgattgtcgt ggacgggtgc
     1021 tcaccagatg gcacgccaga ggtgatggca gattacgctg ggcggaatcc agccattcgc
     1081 tattaccgcg agacagtcaa ttccggggtt gacggtgatt acgaaaaggc cgttgcctac
     1141 gcgcgtggcg attactgctg gctgatgact gatgacgacc tcctcaagcc cggcgcgata
     1201 cgtgtggtgc tcaatgccat tgagcatcat cccgacttgg tcgtgctcaa ctctgaagta
     1261 tgcaatgcgg atttttcggc attattgact ccgcgattca tccagctgaa gcaagacaag
     1321 tcctatcgat ccgatgcagt ggatgtcctg ttcacggaga cggcaggcgc gctgagcttc
     1381 ataggctgtg tggtgattcg acgggaaacc tggatgacca ggatgcgtga agcctatctg
     1441 ggaaccttgt tcattcatat gggggtgatc ttccagaccc cggcgcttga tcaagtgagg
     1501 gtggtggcgg agccgcagat ctcgattcga tatggcaacg ccatgtggac ttcgcgggga
     1561 tttgagatct ggatgt
//
LOCUS       DATMTI010000810         1861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_454850, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000810 DATMTI010000000
VERSION     DATMTI010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454850"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..>1861
                     /locus_tag="VN039_12805"
     CDS             160..>1861
                     /locus_tag="VN039_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HWV46876.1"
                     /translation="MPKAKKSDLSAKVESVAKKSASSPTSAPSAVEDFEKLGVFYLGR
                     PYDLATKQAKPGWLLYDSKDLVTHAVCVGMTGSGKTGLCLALLEEAAIDNIPAIIIDP
                     KGDLGNLMLTFPSLKGEDFQPWINEDDARKKGVSSADYAKAQAELWTKGLAGWQQDGA
                     RIQRLHDAADVAIYTPGSSAGLQVSILKSFAAPVADVREDAELLRERISTTVTSLLGL
                     LGIEADPIQSREHILLSTILDHTWKKEEDLDLAALIQAIQSPPVSKIGVMDMDSFFSS
                     KDRFALAMKLNNLLAAPGFQTWMEGEALDIQQLLYTSSGKPRLAIFSIAHLNDAERMF
                     FVTLLLSQMVGWMRAQSGTTSLRALLYMDEIFGYFPPVANPPSKLPLMTLLKQARAFG
                     LGVVLATQNPVDLDYKGLANTGTWFIGRLQTERDKARVLEGLEGASSSAGKKFDRGRM
                     EQTLAGLGNRIFLMNNVHEDEPVIFETRWCLSYLRGPLTRAQIKTLMESARGKGRETR
                     AVEQRIPVGDAPSRQVSTSAPVTSPPMLSPDIPQHFIPVRSTQPAASALFYQPMLLGA
                     G"
BASE COUNT          408 a          533 c          515 g          405 t
ORIGIN      
        1 tgacggtcgt cggagttaag aacatcccat ctctcgccca caattcatgg gctcatagtc
       61 attccggttg atcgttccgc gcccgcttga agctcatcct gtagaccact acaataccgc
      121 ccaattcatc ccgcccgctg agcgaaggga gactttgtta tgccgaaggc gaagaagtcc
      181 gacttgagtg cgaaagtcga gagtgtcgcg aagaaatccg cctcgtcacc cacctccgcc
      241 ccatctgccg tagaagattt cgaaaaactc ggcgtgtttt atctgggacg tccctatgat
      301 ctcgccacga agcaggccaa gccgggatgg cttctgtatg actcgaaaga tctggtgaca
      361 catgccgtct gcgtcggcat gaccggcagc ggcaagaccg gtctttgtct cgcgctcctc
      421 gaagaggcgg cgatcgacaa tattccagcc attatcatcg atccgaaggg tgatctcggc
      481 aatttgatgc tgacgttccc atctctcaag ggggaagact ttcaaccctg gatcaatgag
      541 gacgatgcgc gtaagaaagg cgtatcatca gcagactatg ccaaggcaca ggctgaattg
      601 tggacgaagg gcttggccgg ctggcaacag gacggtgcca ggattcaacg attgcatgat
      661 gcagcggatg ttgccatata cacgccgggc agcagcgccg ggttgcaggt ttccatcctt
      721 aaatcgttcg ccgcaccggt tgccgatgtg cgtgaggatg cagagcttct gcgtgaacgg
      781 atcagtacga ccgtcacgag tctgctgggt ctgctcggca tcgaggctga tccaatccag
      841 agccgcgagc atattcttct gtccaccatt ctcgatcaca cctggaaaaa agaagaggat
      901 cttgatctcg cggcattgat ccaggctatc caatcgccgc cggtctcgaa gatcggcgtg
      961 atggacatgg attccttctt ttcctcgaaa gatcgcttcg cactggccat gaagttgaac
     1021 aatctgcttg ccgccccagg atttcaaacc tggatggaag gagaagcact cgatattcag
     1081 caactgctgt atacctcgtc gggcaagcca cggctggcga ttttttccat cgcgcatttg
     1141 aatgatgccg aacgcatgtt cttcgtgacg ctgttgctca gtcagatggt cgggtggatg
     1201 agggcgcaat cgggtaccac cagcctgcgc gcgctgctct atatggacga gatcttcggc
     1261 tactttccgc cggtggccaa tcctccctcg aaactcccat tgatgaccct gctcaagcag
     1321 gctcgcgcgt tcggtctcgg tgtcgtgctt gccacgcaaa acccggtgga tcttgactat
     1381 aaggggttgg ccaacaccgg aacctggttc atcgggcgtc tacagacgga gcgagacaag
     1441 gctcgtgtat tggaaggact tgaaggagca tccagcagcg caggcaagaa gtttgaccgt
     1501 ggccgtatgg agcaaacgct cgcgggtctc ggcaatcgca tctttctcat gaacaatgtg
     1561 cacgaagatg agccggtcat ctttgagacg cgatggtgcc tctcgtattt gcgtggcccg
     1621 ttgacccggg cacagatcaa aacgctcatg gaatcggcac gaggcaaggg gcgagagaca
     1681 agggccgttg aacagcgaat acctgtcggg gacgccccat cacgtcaggt ctcaacctcc
     1741 gcgcccgtta cctctccccc tatgctttcg cccgacatcc cacaacactt cattcccgtc
     1801 cgtagcactc agccagctgc cagcgcactg ttctatcaac cgatgctgct gggggcggga
     1861 c
//
LOCUS       DATMTI010000811         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_114789, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000811 DATMTI010000000
VERSION     DATMTI010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114789"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VN039_12810"
     CDS             <1..611
                     /locus_tag="VN039_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46877.1"
                     /translation="LMDGDKRFLKQIEVSKDGGLKNAVVAVVDVEDKAFVDGYAGTEV
                     VAAFCEFQPFSGIVVNTRPFKVENTDADPNDPKSVAGVLHNPHSFTVKGTTSATGFNI
                     GLAKKGDKLDKPVTFRGGAEKEGYFRLQCDQHEFMQSFFLPVSNPHFAVVKDDGSFEI
                     KDVPAGKHKVVAWHPFAGKAKKIEFEVDVTDGGTANLKAEIK"
     gene            complement(712..3168)
                     /locus_tag="VN039_12815"
     CDS             complement(712..3168)
                     /locus_tag="VN039_12815"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWV46878.1"
                     /translation="MAQTPNNPDRRSFDPNDDDFHIRAKDSEPSWGGRDAAKMSAQTG
                     SRMSAISRRQFLRLSSLIAVPLSIAAPSTAPSEDSLFHPSGDRERRLSDLGDPRMGSS
                     LANAVVIKSGNVCFVADADGMVPLAGGHGFGLYYHDCRFLSGYVVRLAGQLPVALSGT
                     AARPFHAKFELTNPRIRTSDGDVLHPDAIGIEWRRVVDDAQPALHDQFFIKNFGRGRA
                     EFLLSFSFRAAFEDVYDVRDALSRTLGVLHPPTWRNGVLGFLYEGKDGFYRSLGIQFP
                     PSVKVTGAQTAHVPIMLEPKESTDFRVSLLLAESRDPHDAQASVRQPSLPEKPDPDVP
                     QASAVWFDTLPLVVSDNLVLNKVITRSFVNLGLLRSQIDHLTFFAAGTPWFATLFGRD
                     SLTAALQMLAYDPSVAEQTLRLLAKYQGQQEDSWRDEEPGKILHELRMGEMARLGLIP
                     QTPYYGSIDATPLFLILIGRHAAWTGSLDLFHELQDSVDRALVWLETAAHRYGHSYLA
                     YMSRPSANQGWKPLDNQGWKDSGEAIVNTDDSLAAPPIALVEVQGYVYRAKLAVAQLY
                     ERTRSVTRAARLRQEASDLRARFNRDFWVAERGFYAMALQGDGRPAVVISSNPGQALW
                     SGIVEENKAKRTVDRLMSDEMFSGWGIRTLSEREQAYNPLGYHIGSVWPHDNSLIAMG
                     FRRYGYDQAACRVFDGLLEAAMHFKSYRLPELFAGFSRSEYEVPVPYAAANPPQAWAA
                     GAVPYLLEAVLGLVPEAFERRLRIVRPTLPGTVDELHIQGMKVGQARVDLQYERTGDG
                     TVGVRVLKVDGALDVVVEQV"
BASE COUNT          730 a         1018 c         1007 g          721 t
ORIGIN      
        1 acttgatgga cggggacaag cggtttctga agcagatcga agtgagcaag gacggcgggt
       61 tgaagaacgc ggttgtcgcg gtcgtggatg tcgaagacaa ggcgttcgta gacggctatg
      121 ccggaacgga agtcgtggcc gcattctgtg aattccaacc gttcagcggt attgtcgtca
      181 acactcggcc cttcaaggtt gaaaacacag atgcagaccc caatgacccc aagtccgttg
      241 ctggtgtgct tcacaaccca cacagcttta cggtgaaggg cacgacctcg gcgaccgggt
      301 tcaatatcgg cttggccaag aagggtgaca agcttgacaa gccggtgact ttccgtggtg
      361 gcgcagaaaa ggaagggtat ttccggttac agtgcgacca gcatgagttc atgcaatcgt
      421 tcttcctgcc cgtgtcgaac ccgcattttg cggttgtgaa ggacgatggt tccttcgaga
      481 tcaaagatgt tcctgccggc aagcataagg tggtggcctg gcatcctttc gcgggtaaag
      541 ccaagaagat agagtttgag gtcgatgtga ctgatggcgg cacagccaac ctcaaggctg
      601 aaatcaagta agcctgtttc atcctgtgct cgtaaccgaa gggcagcgga acccccgctg
      661 cccttcgtgt tttgtctctc ttacgagccg gcgacaagct ttggcccgtt ttcagacttg
      721 ctccacgact acatccagcg cgccgtcgac cttgagcaca cggacgccca ctgtgccatc
      781 cccggtccgt tcgtattgaa ggtccacccg cgcctgcccc accttcatcc cttgaatgtg
      841 cagttcatca acggtgccgg gtaacgtcgg acgaacgatt cggagccgac gttcgaacgc
      901 ttcgggaacc aatcccaaca ccgcctcaag caggtagggg acggctcctg ccgcccaggc
      961 ctgcggcggg ttggcggcgg catacgggac cggcacctcg tactcgctgc ggctgaaccc
     1021 tgcgaacagc tcgggtagcc gataggattt gaaatgcatg gctgcttcca acagcccgtc
     1081 gaacactcgg caggctgcct gatcgtagcc gtaacgcctg aatcccatcg cgatcaaaga
     1141 attgtcgtgc ggccacacgc tgccgatgtg atagcccagc gggttgtatg cctgctccct
     1201 ctccgagagt gtacggatcc cccaaccgct gaacatctcg tcggacatga gtcgatccac
     1261 cgtgcgcttc gccttgttct cctccacgat cccgctccag agggcttgtc ccgggttcga
     1321 tgagatcacg actgcgggcc gcccgtctcc ttgcaaagcc atggcgtaaa atccgcgctc
     1381 tgctacccaa aagtccctgt tgaaccgagc acggagatca gaggcttctt gtcgaagccg
     1441 ggcagcgcgc gtcacactgc gggtacgttc gtataattgc gcaaccgcaa gcttggccct
     1501 gtagacatag ccttgcactt caacaagcgc gatgggcgga gcggcgaggc tgtcgtccgt
     1561 attgacgatg gcctcaccgg aatccttcca tccctgatta tccagcggtt tccatccctg
     1621 attagctgac ggccggctca tgtacgcgag atagctatga ccgtatcggt gtgcggcggt
     1681 ctcaagccat acgagcgccc ggtcaacgga gtcttgcaac tcgtgaaaca ggtccaggct
     1741 gccggtccag gcggcatgac gtccgatcag aatgagaaac agcggcgtcg catcgatcga
     1801 tccgtagtaa ggggtctgtg gaatgagacc gagacgggcc atctctccca tgcgtagttc
     1861 gtggaggatc ttgcccggtt cttcatcccg ccatgaatct tcctgctgtc cctgatactt
     1921 cgccagcaga cgcaacgtct gctcggccac gctcggatcg taggcgagca tctgcaacgc
     1981 agcggtcaga ctatcccttc cgaacaaggt cgcgaaccag ggcgtaccgg cggcaaagaa
     2041 cgtgaggtgg tcgatctggc tccgcagcag tccaagattg acgaatgagc gcgtgatgac
     2101 tttgttcaat accagattgt cgctgacgac cagtgggagc gtgtcgaacc agaccgctga
     2161 ggcctggggc acgtcggggt ccggcttctc tggaagcgac ggctgccgca cgctcgcttg
     2221 cgcatcatgt gggtctcgag actccgccaa gaggagcgac acacggaagt ccgtactttc
     2281 cttcggctca agcatgatcg gaacgtgcgc ggtctgcgcg cctgtcactt tcacggatgg
     2341 cggaaactgg atgcccaggc ttcggtagaa cccatctttg ccttcataca aaaaccccag
     2401 cacgccgttt cgccacgtag gtggatgtag aacccccagt gtccgcgaca gtgcgtcgcg
     2461 cacgtcatag acatcctcga aagcagcccg aaaggagaat gagagcagaa attctgcccg
     2521 tcctcgcccg aagttcttga tgaagaactg gtcatgtagg gccggttgcg catcatcgac
     2581 gacacgccgc cactcaattc caattgcatc cgggtggagg acatcgccgt cggatgtcct
     2641 gattcgcgga ttcgtcaact cgaactttgc gtgaaagggg cgcgcagccg taccgctcag
     2701 ggcgaccgga agctggccgg ccaaccgaac gacgtatccg ctgaggaacc ggcaatcatg
     2761 atagtacaag ccgaacccat gtccgccggc cagtggcacc atgccgtccg cgtccgcgac
     2821 aaagcacacg ttgccgctct tgatgactac ggcgttggcc aagctgctcc ccatacgggg
     2881 atcaccgaga tccgacaacc ggcgttcccg atcaccagac ggatggaaga gtgaatcctc
     2941 ggacggagct gtggaggggg cggcgatcga gagcgggacg gcgatcagcg aagaaaggcg
     3001 aagaaactgt ctgcgcgaga tcgcagacat tctgctcccc gtctgagcgg acatcttcgc
     3061 ggcgtctcgt ccgccccacg acggttcaga atccttcgct ctgatgtgga agtcgtcgtc
     3121 gttcggatcg aaactccttc ggtcagggtt gttgggtgtt tgtgccatgc cccttgctga
     3181 tcgttaacga cctggtcacc gctaagtcaa cctcgaaata ggctgcgaat tattattgta
     3241 tcgtaaataa aacatatgtg cattcgatga agctggccac tggtaaagtc cctatgctgc
     3301 cagactgtat ttcgtcaggg gggtcatgca tgcacagccg cctccctcag ctcttggcgg
     3361 acgaggtgtt gaagcgcggg ccgtcacttt cacgattagc gatgacgagt tcagcgcctt
     3421 gtcttttact ttccgatcta ggtaagatcc tgacattcag agctatttgc tcgagg
//
LOCUS       DATMTI010000812        10753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_327866, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000812 DATMTI010000000
VERSION     DATMTI010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327866"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VN039_12820"
     CDS             <1..364
                     /locus_tag="VN039_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46879.1"
                     /translation="LKGPDMAKVDDLVNSVGVSEEDGITPLYPDTFRDDILGASAEDA
                     ALAAAELSAVTAQRDAAAAEVVTLKAYIADYIVGSVAEGDTDDSDDNSGADNESDDEP
                     ESSGVAGLFAKMAESTKD"
     gene            370..2025
                     /locus_tag="VN039_12825"
     CDS             370..2025
                     /locus_tag="VN039_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46880.1"
                     /translation="MKLNDLPTVPWDPSIDNSDLIAAVRDEASNEYQRRIPDAIKGDT
                     EATLRALNNYQPNWNEFTSVLLNLVAMQVIRSNSWQNPFAMFKKGMVTNGDTVEEIQT
                     GLVRGYLYDPRREYGEKALFGKHLPEVQTSFHKKNRESMYPISVQRPTLQRAFLTDGG
                     LGDFISNIIDSLNTSDNLDEFLLMTQTLRLYADMDGFFKVHIADIASPSSTSDDARSV
                     LRTLREYADTLPFISRHYNAAKMPVVAAPEDLYLICTPQFKSSLDVNGLAQLFNVSYG
                     DVNYKTVVIPRQYMPDTIEGILTTKDFFQVYDLYFDTAVQPNVAGRYENYFLQHDQII
                     SASRFVPAIAFTTGEGDVIDVTETPVTGVTAITITDITGATVTEVQRGGFYLVNASAI
                     TTGDNTGVDLEQAQPHSNFTTLAQSGTFMVSLDEKAFDLTITATATDGRNFSSTLTVP
                     VVGERVDFWPNPSVMPDTDDDGLLEVTPTAPAWDVATNTITIPDAQEGVGYKNGATVL
                     TPGETIVVADGTPVTITAYLPDATHYEFAAGAVTSWTFTFGVA"
     gene            2110..4623
                     /locus_tag="VN039_12830"
     CDS             2110..4623
                     /locus_tag="VN039_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46881.1"
                     /translation="MGFKRDPQSNQYDAGDGFNYSVWTANTELTLANVPWDAAYKDVV
                     HFDSTDDLNTYLDGRGDSTTISDVMYARIDEPIIIDMPFAVANQYNYIRAFNPAQPVS
                     GGDIAKYYYYFIVGMVHDAPNTTRIIVQLDVWQTWIRQIQFGMAYINRGHIGVANQNK
                     GNNYGRDYLTIPDGLDTGSDYVNVTSRRRNLLDTSGTFKDYNVLAFSTVDLNAPSYLD
                     PPTNSKPNNPTARGTIFGNLISGAMGFVWTSPIDFLNFMGKYSQYPWMTSGIISITLI
                     PSVAALGYTFQSGKDADTGAYKLVGAPSFNRRLFPNWRDSDDVMDYIPDRYKIFKEKL
                     LTAPYCMLELTAQGGSALVMKPELWNSAHASVMETSSFLPPNQRIVYYPEGYNGRNYG
                     DGWADNQAVPADNIYLQDGDNLDMSLIISSFPTIPIVNNGQLEYLAQNAHSIAAQYQS
                     LDYSQTKALRGNQVGAENTTVGMNAGMSGTANSVAGDMNQLAIQQQLQAQTAMFNLLG
                     SVGAGGAGGAAGGPIGMGAGAATAAGGGLANLATLNMQQDAANRAYNARTNTAYGAAK
                     ISYGAAGQVRDNNLAYGNWAARGDYENARRMMDGKIQDTRMIQPGVSGGFGGDAYLFN
                     TRQMYLTFKIKMVDQASMARAGEFWLRYGYPVGRPSMIPNDLRVMTKFSYWQLTECYI
                     LNGAMPESHKQTVRGVLEKGVTVWTDANDIGQIDFADNAPLDGIVIGGYTPPAWTPEP
                     PPTPPTPAKRKNKKMLVYTTVDGTPIWGLAGSAGAGVPANFLTTRDAVLAGAWLEACG
                     VTEPVAVDTTTFYDYQTAYQAQPNVLANAVQPFYTTTAP"
     gene            4623..5609
                     /locus_tag="VN039_12835"
     CDS             4623..5609
                     /locus_tag="VN039_12835"
                     /inference="COORDINATES: protein motif:HMM:NF017190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46882.1"
                     /translation="MSRNKRRDNVAEDYYSILRARTKQRANPGITDLEILYQMYERIL
                     GELAMNRFKWSGFPDTVNTRFVEMILLLRGLVVVFKDIKDTGKKDTPGFRPHTDAIFA
                     LRGTPSGQRNMMDDPTEYTITGPGTGQHFQGLQLKATACVPIWANYFRTTDYDIITTY
                     ARKLANVDRTIEINLRNARRTKVLTYDENSRLSIQNIAAQIDEGAATIAVTREALMNN
                     SITALDLGVNPDSIEKLSIMRARLWSEAMGLLGINNANQDKKERLVADEVDANDDQVE
                     SMRRVNLNARQQACDLMRRMFPEELGNVSVEFYSQDAQIPAGPADTMGEDDA"
     gene            5602..6321
                     /locus_tag="VN039_12840"
     CDS             5602..6321
                     /locus_tag="VN039_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46883.1"
                     /translation="MPSFTLELWRVLDGKNPSKTEDQELRLDTYPIFDETYRVGLNTK
                     IKNHFMYREIGQETVEQFTFMLGRKMNEIMPYYNQLYTSERIVFDPLSTVDMSTVTTG
                     TGHATAEDTATSEQSSTGESKAKTVNSAFPQMQLQPDKDYATSGADSISDTTNSGTGT
                     NTSNSTNDSTTSGNTTTSGYQGPASDLLTRFRDTFLNIDMQVIAELEPLFMQIWNTDD
                     SYTTRQSSFHYYAGYPGGIFY"
     gene            6340..8157
                     /locus_tag="VN039_12845"
     CDS             6340..8157
                     /locus_tag="VN039_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46884.1"
                     /translation="MTTIPPITGIPVVNYPDFQAYTQITPFTVRDGATYLLTLESLKD
                     WIRDVLVPHLDSQVEELAAAWQTNADDIITAFNSAVTSLTASVNDAIAQITDQVEQAN
                     AAVTAAQAAATAAAGSATEAEGWAGAAEAFQDTAMATLVNNVASELRAALDAIYPTIA
                     SVAATYQAKHTVVVTAAGDAAVNTTAANAALNTGADVILAALDGTPFTTNATLTFKSS
                     VVGQKLSTAGTIITPTFHGDVVQITNAEQEAHVRIQAGSQPAGDYTEVAAIRIGSADG
                     VWNAKNASVSRSVVLGAWQGNGVILEQGAHVDFTGFSVTLSVSVDGIRGTSNADDSNE
                     AYLASTRISNAARYGYYLMNNATNPNNSSRSWEFINAKAFGCGTNFRIETSQNHGSIF
                     SENGINPDTFTATSIANKIDYTATNASFVSVSDLGSGNEISGRNSDSLWDTIVKRVRS
                     LLVHVPGFSGSQQFTQSASYTFTDSLTGSSQPMTVIHQHGTAGSVRTDQFDSQLSLPL
                     TAGGNRAIIRNASLIQYTFVTADGPIAVGALTQIQIGSGFGAKSLAVCQLYGSAARDL
                     DYRVWVHSAGSMYLRVKNDGTASVDPTGIVASVMVLNLV"
     gene            8154..9281
                     /locus_tag="VN039_12850"
     CDS             8154..9281
                     /locus_tag="VN039_12850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWV46885.1"
                     /translation="MSVISIRRAGTDFLATNDDGTVARLIGTQGDVWIGAQVVPDDTS
                     GATGSGSGLVLGPIPSTLTATAKDGTVVTLNSTQLNNAGTIIKNAARNDRVGRKGALI
                     ALMAALTESSLLMYANSNVPESETYPHDAVGSDHDSLGFFQMRPSTGWGSVANLMNGD
                     YNCRAFFGGASGPNYPSPRGMLDINGWQTMDPGTVCQAVEVSAFPDRYQNFQPVAQAI
                     LNALTVTEKPGTIPPSTGTRFIWPFPLSVVTSEFGMRNGRLHAGMDFSGSPAVAGATI
                     PASANGIVENTWTTGQHGGYGNVVLLNHGSGLFTLYAHMQDNSFMVRKGQTVHQGDGL
                     GKLGNTGQSYGAHLHFETHEGGYRWDASAVNPRVFIPKYNA"
     gene            9278..10393
                     /locus_tag="VN039_12855"
     CDS             9278..10393
                     /locus_tag="VN039_12855"
                     /inference="COORDINATES: protein motif:HMM:NF017687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA encapsidation protein"
                     /protein_id="HWV46886.1"
                     /translation="MNIGTHAPARRPPAKKRRQTRVHPYYSYNKILSVNAMFNFIVGG
                     RGLGKTYGAQKLAIKKALRTGDQFMYVRRYKEELKAAKDTFFAAIQNEFPEWEFRVNG
                     PRAEATEYIEQEPDETAAQFAKRKQQRYWQTIGFFQALSVAQQMKSTAYPDVTLIIFD
                     EFIIETGTNVSYLRDEVNAFLNLFNTVDRTEDKTRALFLANSVSIMNPYFIYWEIWPD
                     KLAEFSTMKNGDIALHFPTSKAFATSVYETRFGKFIAGTDYANYAVGNEFRDNGEALI
                     AQKTKDARYNYTLETKGGVFSVWRDPFRTMTYILDRRVGNEQFYTMLASRMAEGKQVV
                     TFNEPVLAILRRDFNRGLVMFDKPSTRNAFVPIFERR"
     gene            10396..10599
                     /locus_tag="VN039_12860"
     CDS             10396..10599
                     /locus_tag="VN039_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46887.1"
                     /translation="MPPSAGEKLEREIGVVVTIKEIHDTVLRIDDTLTSEISKLKARI
                     AAHEVVIGMMTLVIVYLIQKGIA"
BASE COUNT         2466 a         3267 c         2899 g         2121 t
ORIGIN      
        1 attgaaaggg ccagacatgg ctaaggttga cgatctggtc aattccgtag gcgtgtccga
       61 agaggacggg atcactcccc tctatccgga cacgttccgt gacgacatcc tcggcgcatc
      121 cgccgaagat gcggcactcg ccgccgccga actgtccgct gtcaccgcac agcgcgacgc
      181 cgccgcggct gaggtggtga cgcttaaggc gtacattgcc gattacattg tcgggtctgt
      241 cgctgagggc gacaccgacg acagcgacga caacagcggc gctgacaacg agtccgacga
      301 tgagcccgaa tcttccgggg tcgctggact cttcgccaag atggcagaaa gcactaagga
      361 ctgactacag tgaagcttaa cgaccttccc accgtaccgt gggaccccag catcgacaat
      421 tccgacctca tcgccgccgt ccgcgacgag gcatccaacg agtatcagcg ccgcatcccc
      481 gacgccatca agggcgacac cgaagcaacc ctccgtgccc tgaacaacta ccagcccaac
      541 tggaacgagt tcaccagcgt cctgctgaac ctcgtggcca tgcaggtcat ccggtccaac
      601 tcgtggcaga atcccttcgc catgttcaaa aagggcatgg ttaccaacgg cgacaccgtc
      661 gaagaaattc agacgggcct tgttcgcggc tacctgtacg acccccgccg tgagtacggc
      721 gagaaggcac tcttcggcaa gcatctgccc gaagttcaga cgtccttcca caagaaaaac
      781 cgcgagtcca tgtaccccat ttcggtacag cgccccaccc ttcagcgcgc gttcctcacc
      841 gatggtggtc tcggcgactt catttcgaac atcattgaca gcctgaacac gtccgacaac
      901 ctggacgagt tcttgctgat gacgcaaacc cttcgtctgt acgcggacat ggatggtttc
      961 tttaaggtgc acattgcgga catcgcttcg ccgtccagca cttcggacga cgcccggtcg
     1021 gtgcttcgta ctctgcgcga gtacgcggac actctcccgt tcatttcgcg acactacaac
     1081 gccgcgaaga tgccggttgt cgccgcacct gaggatctgt acctcatttg cacgcctcag
     1141 ttcaagtcca gtctggacgt gaacggtctc gcccagctgt tcaacgtgtc gtacggcgat
     1201 gtcaactaca agacggttgt catcccgcgc cagtacatgc ccgacaccat cgaagggatc
     1261 ctcaccacga aggacttttt ccaggtgtac gacctgtact tcgacacggc ggttcagccg
     1321 aacgtggctg gccgctatga gaactacttc cttcagcacg accagatcat cagcgcatcc
     1381 cggttcgttc cggctatcgc attcaccacg ggagaaggcg acgtaatcga cgtgaccgag
     1441 acccccgtca ccggcgtgac cgccatcacg attaccgaca ttaccggcgc aacggtgacc
     1501 gaggttcagc gtggcggatt ctaccttgtg aacgcatccg ccatcacgac gggtgacaac
     1561 acgggcgtgg atctggaaca ggcccagccg cattccaact tcacgaccct cgcccagtcg
     1621 ggcacgttca tggtgtcgct ggatgagaag gcgttcgacc tcaccatcac ggcaacggca
     1681 acggacggtc gcaacttctc gtccacgctc acagtgcccg tcgtcggcga gagggtcgac
     1741 ttctggccca acccgtcggt catgcctgac accgacgatg acggtctgct ggaagtcacg
     1801 cccacagcgc ccgcttggga cgtggcgacc aacaccatca ccatccccga cgctcaggag
     1861 ggcgtgggat acaagaacgg tgcgacggtg ctcacccccg gggagaccat cgttgtcgcg
     1921 gatggtaccc cggtcaccat cacggcgtac ctgcccgacg cgacgcacta cgagttcgcc
     1981 gctggcgcgg tcacctcgtg gacgttcacc ttcggtgttg cctgactgat aggctaggca
     2041 tcacgcccca cccgggagac ttccccgccc gggtggggca ccctcacaac ttcacggaaa
     2101 gcgcggtcca tgggattcaa acgagacccc cagtcaaacc agtacgacgc tggcgacggt
     2161 ttcaattatt ccgtttggac ggcgaacacc gaacttaccc tcgcaaacgt gccgtgggat
     2221 gccgcctaca aagacgtagt gcatttcgat tccacggacg acctgaacac gtaccttgac
     2281 ggccggggcg attccacaac catttcggat gtgatgtacg cccgcatcga tgaacccatc
     2341 atcatcgaca tgccgttcgc tgtcgcgaac cagtacaact acattcgcgc gttcaacccg
     2401 gcacagcccg tttccggcgg cgacattgca aagtattact actatttcat cgttggcatg
     2461 gtgcacgacg ccccgaacac cacacgcatc atcgttcaac tggacgtgtg gcagacgtgg
     2521 attcgccaaa tccagttcgg tatggcctat atcaacaggg gtcacattgg cgttgccaat
     2581 cagaacaagg ggaacaacta cggacgtgac tacctgacga ttcccgatgg tctcgatacc
     2641 ggatccgatt atgtcaatgt cacgtctcgc cgtcggaacc ttcttgacac atcagggacg
     2701 ttcaaggatt acaacgttct tgcgttctca actgtcgatc tgaacgcccc atcgtatctg
     2761 gacccgccga ccaacagtaa gccgaacaac ccgaccgcac gcggaacgat cttcggtaac
     2821 ctcatttccg gggcaatggg tttcgtgtgg acgagtccta tcgacttcct gaatttcatg
     2881 gggaagtatt cccaataccc gtggatgact tccgggatca tcagcattac cctcatcccc
     2941 tccgtcgcgg cactaggtta cacgttccag tccggcaagg atgccgacac gggcgcctac
     3001 aaactcgttg gggcaccgtc gttcaaccgt cgcttgttcc cgaactggcg tgactccgat
     3061 gacgtgatgg attacattcc ggaccgctac aagattttca aagagaaact tctcactgcg
     3121 ccctattgca tgctcgaact gaccgcacag ggtggttcgg ctctggtgat gaaaccggaa
     3181 ctgtggaaca gtgcgcacgc atcggtgatg gaaacgtcat ctttcctgcc cccgaatcag
     3241 cgcattgtct actatccgga agggtacaac ggacgcaact acggtgacgg atgggcggac
     3301 aaccaggcgg tccctgccga taacatctat ttgcaggatg gcgacaatct ggacatgtcg
     3361 ctcatcattt ccagttttcc gaccatcccc attgttaaca acggtcaact ggaatacctc
     3421 gcacagaatg cgcacagcat cgccgcgcaa taccagtcgc tggattactc ccagacgaag
     3481 gctctacgcg gaaaccaggt cggcgcagag aatacgactg ttggtatgaa tgcgggcatg
     3541 tccggcacgg cgaacagcgt tgccggtgac atgaaccaac ttgccattca acagcaattg
     3601 caagcacaaa ccgccatgtt caacctactc ggatcggttg gcgcgggcgg cgcgggcggc
     3661 gcggcaggtg gaccgatcgg catgggcgcg ggtgcggcca ccgcggcagg tggtggcctc
     3721 gcgaacctcg caaccctgaa catgcaacag gacgccgcga accgggcata caacgcacgc
     3781 acgaacacgg cgtacggtgc cgcgaaaatc agttacggtg ccgcaggtca agtgcgtgac
     3841 aacaacctcg catatggcaa ctgggccgcg cgtggcgact atgagaacgc ccgtcgcatg
     3901 atggacggga agattcagga cacgcgcatg attcagcccg gcgtttcggg cggtttcggc
     3961 ggcgacgcct acctgttcaa cacacgccaa atgtacctga cgttcaagat taagatggtg
     4021 gaccaggcgt ccatggcccg cgcgggcgaa ttctggttgc gttacgggta ccctgttggg
     4081 cgcccgtcga tgatcccgaa cgatcttcgc gtgatgacga agttctccta ctggcaattg
     4141 acagaatgct acattctgaa cggtgccatg ccggaaagtc acaaacagac cgtacgcggc
     4201 gtgctcgaaa agggcgtcac ggtctggacc gacgccaacg acatcggaca gatcgacttt
     4261 gccgacaacg ccccacttga tggtattgtc atcggaggat acacaccccc ggcatggacg
     4321 cccgaaccgc cgcccacacc tccgacccct gccaagagaa agaacaagaa aatgcttgtt
     4381 tacaccaccg ttgacggcac cccgatctgg ggccttgccg gttccgcggg tgcgggtgtc
     4441 cccgccaact tcctgaccac ccgtgacgcc gtcctcgcgg gcgcatggct tgaagcatgc
     4501 ggggtcacag agcccgtcgc cgtggacacc accacgttct acgactacca gaccgcctac
     4561 caggcacagc cgaacgtgct cgcaaacgca gttcagccgt tctacaccac cacggccccg
     4621 taatgagtcg gaacaagcgt cgcgacaatg tggcagagga ttactattca atcctccgtg
     4681 cacgtaccaa acagcgcgcg aatccgggta tcacggatct ggaaatcctg taccagatgt
     4741 atgagcggat tcttggcgaa cttgccatga accggttcaa atggtcgggt ttccccgata
     4801 ccgtgaacac acggttcgtg gaaatgatcc tcttgcttcg tggtctcgtg gtcgtgttca
     4861 aagacattaa agacacgggt aaaaaggaca ctcccggttt ccgaccgcac acggatgcta
     4921 tcttcgcgtt gcgtggcacg ccttccggtc agcggaacat gatggacgat cccacggaat
     4981 acaccatcac gggaccggga acaggccagc attttcaagg cttgcaactg aaggctacgg
     5041 cgtgtgtgcc gatctgggcg aactacttcc gcaccacgga ctatgacatc atcaccacgt
     5101 acgcccgcaa actggcaaac gtggaccgca ccatcgaaat caaccttcgc aatgcacgac
     5161 gtacgaaggt gctcacgtac gacgagaatt cgcggttgag cattcagaat attgccgcgc
     5221 agatcgacga gggtgcggcg acaatcgctg tcactcgtga ggcgttgatg aacaactcga
     5281 ttacggcgct ggacctcggc gtcaaccctg acagtattga aaaactgtcc atcatgcggg
     5341 cacgtctttg gtccgaagcg atgggactgc tcggcatcaa caacgcaaat caagacaaga
     5401 aagaacgcct tgtcgcggat gaggtagacg cgaatgacga tcaagtagag tccatgcgcc
     5461 gtgtcaacct gaacgcgcgt caacaggcgt gcgacctcat gcggcgcatg tttcccgaag
     5521 aactggggaa tgtgtctgtt gagttctatt ctcaggacgc acagatcccc gcgggacccg
     5581 ccgacacgat gggagaagac gatgcctagt ttcactctcg aactgtggcg agtgctggac
     5641 gggaagaacc cgtcgaagac agaggatcaa gaactacgac tcgacacgta cccgattttc
     5701 gatgagacct atcgggtcgg gttgaacacg aagatcaaga accatttcat gtaccgggaa
     5761 atcggacagg aaacggtgga acaatttacg ttcatgcttg ggcgcaagat gaacgaaatc
     5821 atgccgtact acaaccagtt gtacacctcc gagcgaatcg tgttcgaccc gctgtccacc
     5881 gtggacatgt ccaccgtgac aaccggaacc gggcacgcca ccgcggaaga caccgcaaca
     5941 tcggaacagt ccagcacagg tgaatccaaa gccaagacgg tcaacagcgc attcccccag
     6001 atgcaactac agcccgacaa ggattacgcc acgtcgggag ccgattctat ttcggacacg
     6061 acgaacagcg gtacgggtac gaatacgtcg aacagcacga acgattcgac cacgtcgggc
     6121 aatacgacca cgtcaggtta tcagggtccc gcgtcagatc tgctgacgcg ttttcgtgat
     6181 acgttcctga acatcgatat gcaggtcatc gccgaattgg aacccctgtt tatgcagatc
     6241 tggaatacgg acgactctta caccacccgc caatcctcat tccactatta cgccggttac
     6301 cctggtggaa ttttctattg agaaaagaga acttaacaaa tgactaccat tcctcccatc
     6361 acaggtattc ccgttgtcaa ctaccccgac tttcaggcat atacgcagat caccccgttc
     6421 accgttcgtg atggcgcgac ctatctgctc actctcgaat cactgaaaga ctggattcgt
     6481 gatgttctcg ttccgcattt ggattctcag gtcgaagaac ttgcggccgc gtggcagacc
     6541 aatgcggacg acattattac tgcattcaac agtgcggtaa cgtcgctcac tgcaagtgtg
     6601 aatgatgcta ttgcccagat caccgatcag gtggaacagg caaacgcggc tgtcactgcg
     6661 gcacaggccg ccgcgactgc cgcggcgggg tctgccactg aggctgaagg gtgggcaggg
     6721 gccgcggagg catttcagga cactgcaatg gctacgctgg taaacaatgt tgcaagtgaa
     6781 cttcgtgcgg cacttgacgc gatttatccc acaattgcca gtgtcgcggc aacctatcag
     6841 gcaaaacaca ctgtcgttgt caccgccgcc ggcgacgccg ccgtcaacac caccgccgca
     6901 aacgcggcac tcaacaccgg tgctgacgtg attcttgccg cactggatgg cacgcccttc
     6961 accaccaatg ccacgttgac ctttaaatca agcgtcgtcg ggcagaaact cagcaccgct
     7021 ggcacgatca ttacgcccac cttccatggt gatgttgtgc agatcaccaa cgcggaacag
     7081 gaagcacacg tacggattca ggcgggatcc cagcccgcgg gcgactacac ggaagttgcc
     7141 gcaattcgaa tcggtagtgc cgacggtgtg tggaacgcga agaatgccag cgtttctagg
     7201 tctgttgttc tgggagcatg gcagggcaac ggtgtcattc tggaacaggg cgcgcacgtc
     7261 gatttcaccg gtttttctgt gacactcagt gtcagtgttg acggcattcg cggcaccagc
     7321 aacgccgacg actcgaacga agcatatctc gcatcgacac gtatcagtaa cgcggcacgc
     7381 tatggttact atctgatgaa caatgcgacg aatcctaaca actcgtcgcg ttcgtgggag
     7441 ttcatcaacg ccaaggcgtt tggttgtgga acaaacttcc gtattgaaac tagtcagaac
     7501 cacggttcga ttttcagtga aaacggtatc aacccggaca cgttcacggc gacatccatt
     7561 gcaaacaaga ttgactacac tgccacgaac gcttcgtttg tcagcgttag tgatcttggt
     7621 tcgggtaacg agattagcgg acgcaacagt gacagtctat gggataccat cgttaaacgg
     7681 gtgcgcagtc tactcgtaca cgttccgggt ttcagcggtt cccagcaatt cactcagtct
     7741 gccagttaca catttaccga cagtctcacc ggcagtagcc agcctatgac cgtgattcac
     7801 cagcacggta cggcaggttc ggtaagaacc gaccagttcg attctcagtt gtcactgccc
     7861 cttaccgccg gagggaaccg tgcgatcatt cgcaatgcct ccctaatcca gtacacgttt
     7921 gtaaccgcgg acgggcctat cgcggtgggc gcgctgacac aaattcagat cggttcgggt
     7981 ttcggggcaa agtctctcgc cgtgtgtcag ttgtacggta gtgccgcccg tgaccttgat
     8041 taccgtgtgt gggtgcacag cgcaggatca atgtacctcc gcgtgaagaa cgacggcacc
     8101 gcgtctgtgg acccgacagg tatcgtggcg agtgtgatgg tgttgaacct cgtatgagtg
     8161 ttatttcgat caggcgtgca ggtaccgact ttctcgcgac caacgatgac ggtacggtcg
     8221 cacgattgat cggaacgcag ggggatgtgt ggatcggcgc acaggtagtg ccggacgaca
     8281 cgtcaggggc cacgggatcg gggtcgggcc ttgtgctcgg ccccatcccc tccaccctga
     8341 ccgcgacagc gaaggacggc accgttgtca cactgaacag cacgcaactg aacaacgccg
     8401 gaaccatcat caagaacgcc gcacgcaacg acagggtggg ccgcaagggg gcgcttatcg
     8461 cgttgatggc ggcgctcact gaatcctcgt tgctcatgta cgccaatagc aacgtgcccg
     8521 aatccgagac gtacccgcat gacgcggtcg gttccgatca tgacagtctc ggtttcttcc
     8581 aaatgcgccc ctccaccggg tgggggtccg tggcgaacct gatgaacggc gactataact
     8641 gtcgtgcgtt ctttgggggc gcgtccgggc cgaactaccc gtcacctcgg ggcatgctgg
     8701 acatcaacgg atggcagacc atggacccgg gcaccgtctg ccaagcggtc gaagtgtccg
     8761 ccttccccga ccgctaccaa aacttccagc ctgtcgcgca agccatcctg aacgccctca
     8821 ccgtcacgga gaagcccggc accatccccc catccaccgg gactcggttc atctggccgt
     8881 ttcccctgtc cgtggttacc tctgagttcg gcatgcgcaa cgggcgtttg catgccggga
     8941 tggacttttc cgggagtccc gcggtggccg gggcgacgat ccccgcctct gccaacggga
     9001 tcgtagagaa cacgtggacc accggtcagc atggcggcta tggtaacgtg gtactgttga
     9061 atcacggatc gggcctgttc accctgtatg ctcacatgca ggacaacagt ttcatggttc
     9121 gtaagggaca gacagttcat cagggggacg gcttgggcaa gttgggcaac acgggccaat
     9181 cgtacggggc gcacctgcat ttcgagaccc acgaaggcgg gtaccgttgg gatgcatccg
     9241 ccgtcaatcc gcgcgttttc attccgaagt acaacgcatg aacatcggca cacacgctcc
     9301 cgcacggcgt cccccggcga agaaacgccg ccagacgcgc gtgcaccctt actactcgta
     9361 taataagatc ttgtctgtca atgcgatgtt caatttcatt gtgggcgggc gtggactcgg
     9421 caagacgtac ggggcacaga aacttgccat caaaaaggct ctccgtacgg gtgaccagtt
     9481 catgtacgta cgccgttaca aagaggaatt gaaagcggcg aaagacactt tcttcgccgc
     9541 gatccagaac gagtttcccg aatgggaatt ccgcgtcaac ggtccccgtg ccgaagcgac
     9601 cgagtacatt gaacaggaac ccgacgaaac agcggcacag ttcgcgaagc gcaagcaaca
     9661 gcgttactgg cagaccatcg gattctttca agcgctcagt gttgcgcaac agatgaaatc
     9721 caccgcctat ccggacgtga cactcatcat ctttgacgag ttcatcatcg aaacgggcac
     9781 gaacgtctcc tacctccgtg acgaggtgaa cgcgtttctc aacctgttca acaccgtaga
     9841 ccgcacagaa gacaagacac gcgcattgtt tctcgcgaac agcgtgtcga tcatgaaccc
     9901 gtatttcatc tactgggaaa tctggccgga caaactcgcc gagttctcca ccatgaaaaa
     9961 tggtgacatc gcactacact tccccacgtc caaagccttc gccacctcgg tctatgagac
    10021 ccgattcggg aaattcatcg cgggaaccga ttacgccaac tatgcggttg gcaatgagtt
    10081 ccgcgacaac ggcgaagcgc tcatcgccca gaagaccaaa gacgcccgtt acaactacac
    10141 cttggaaacc aagggtggtg tgttctccgt gtggcgggac ccgttccgta caatgacgta
    10201 cattctcgac cgccgagtcg gaaacgaaca gttctacacc atgcttgcat cccgcatggc
    10261 tgagggcaaa caggttgtca cattcaatga acccgtcttg gcgattctcc gtcgcgactt
    10321 caaccgtgga cttgtcatgt tcgataagcc atctactcgg aacgctttcg ttcccatttt
    10381 tgagaggcgc tgataatgcc cccgtccgca ggtgaaaaac tagaacgtga aatcggcgtt
    10441 gtcgtgacca ttaaggaaat ccacgatacc gtgctacgca tcgatgacac tctcacgtcc
    10501 gaaatctcaa aactcaaggc gcgcattgcc gcgcatgagg tcgtcattgg tatgatgact
    10561 cttgtcatcg tctacctcat ccagaaaggc atcgcatgac tgacaatgtt cccccgcccg
    10621 tcctcgcatc atccctgacg ccgcgcactg actatcagga atccctcccg aagacggttc
    10681 ggatcacgtc cacgaagttc cggcgcatcc tgtcttacag tatgtacgca ctcgcgggcg
    10741 tgtctggtgc ggc
//
LOCUS       DATMTI010000813         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_327876, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000813 DATMTI010000000
VERSION     DATMTI010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327876"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VN039_12865"
     CDS             complement(<1..1626)
                     /locus_tag="VN039_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46888.1"
                     /translation="MRQKAVLLTGDRLCGIESRAARLLEFFGVRHDTQSAIDFTLPKN
                     PGPERQYRIIGTAQSFESVANRLQTCDGLYDQLHSIFLFSNGDPVGLSKVVSQLAGND
                     ISVRRGAKADLEWTIADDPNALHGVMRGLSVHPTPAAVSDADLFEASEKFAASLIASE
                     EKAAFIRLRWRGVPVFVSSVPLVNIDADLTTQNFDVRDHLFDAVPFVCYLKWAFGDSA
                     WHAPEAGACVVVDDPLLKPRYGFVRYRELLALMKQVRFATTIAFIPWNWRRSDPDVVQ
                     LFKENVKNYSVCVHGCDHTAQEFASSDRQWLRSRASAACERMSGHQTLTGLEHDRVMV
                     FPQGAFSAEAIGELKSAGFDAIVNTEVHSNGGGPRRLTIADVWDVAVMSYDDFAVYTR
                     RYPSQGVENFAFDLLLGKPGIIVIHHDFCKDRCSRLVQFIHQLNSLRTPLTWRCLGDV
                     VKRSYRQKKLTPDLIEIEIYSNQAVIENTSNNTQRYVVRRRERQPDTIDCVRAGSEEA
                     AWHPDGDYIQFEVTLQPGETILLAVRFKAAGPAE"
     gene            complement(1665..>2126)
                     /locus_tag="VN039_12870"
     CDS             complement(1665..>2126)
                     /locus_tag="VN039_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46889.1"
                     /translation="TLIDELTATTGVPQADLVNSFQFVIGVNTIRYCHRANTEAECAR
                     DIWRLLMPGGVCVVIDMNDRFPAFRSALKDKLRGNKRPQRECYLPSLEEYTAPFTRAG
                     FQVLRTGRFCWIPHSAGRFLTGICRTFSPVLTNLGRSRAMRSLVIAQKPSV"
BASE COUNT          504 a          589 c          553 g          480 t
ORIGIN      
        1 ttcagccggc cctgccgctt tgaagcgcac cgccaggaga atggtttcac ccggttgcaa
       61 cgtcacttcg aattggatgt aatcaccgtc cggatgccac gcagcttcct cggaaccggc
      121 acgaacgcag tctatggtgt caggctgccg ttcgcgccga cgcacaacat acctctgtgt
      181 gttatttgag gtattctcta taaccgcctg attcgaatag atctcaatct caatcaaatc
      241 gggcgttagt ttcttctgcc gatagcttcg tttgacaaca tcaccgaggc aacgccacgt
      301 cagaggagtt cgcagtgagt tcaattgatg gatgaactgg actagtcgcg agcatcggtc
      361 tttgcagaag tcatgatgaa tgacaatgat acccggttta ccgagcaaaa gatcgaatgc
      421 gaaattctcc acgccctgac ttgggtaacg ccgcgtgtag acagcgaaat cgtcgtagct
      481 cattacagca acgtcccaga cgtcggctat cgtcagtcgc cgtgggccac cgccattgct
      541 atgaacctca gtgttaacaa ttgcgtcgaa tccagcgctt ttcagttcgc caatcgcttc
      601 cgcagaaaac gcgccctgcg gaaagaccat gacccgatcg tgctctaaac ctgtgagcgt
      661 ttggtgacca gacatccgct cacaggccgc ggacgcgcgt gaccgaagcc actgccggtc
      721 tgaactggcg aattcctgag ctgtatgatc gcatccgtgc acgcaaaccg agtagttctt
      781 gacgttctct ttaaatagtt gcaccacgtc cggatcgctg cgacgccagt tccacgggat
      841 gaatgcgatt gtggtggcga atcgcacctg cttcatcaaa gcaagcaatt cacggtaacg
      901 aacgaagcca tacctcggtt ttagcaaagg gtcatccaca acgacacatg cgcctgcttc
      961 gggagcatgc caggcagagt caccaaaggc ccatttgagg taacaaacaa acggcacggc
     1021 gtcgaacaga tggtcacgga cgtcgaagtt ttgagttgtg agatcagcgt cgatgttcac
     1081 caatggaact gaggaaacga aaacgggtac tcctcgccac ctcagcctta tgaatgcagc
     1141 tttctcctca ctggcgataa gagaagccgc aaatttttcg ctggcttcaa aaagatcggc
     1201 gtcacttacg gcagcaggcg tggggtgcac gcttaatccc cgcatcacgc cgtgcaatgc
     1261 attgggatca tctgcgatgg tccattcgag gtcggccttc gctcctctgc gaacggaaat
     1321 gtcatttccg gccagctggc tcacgacctt tgaaaggccg acagggtctc cgttcgaaaa
     1381 caaaaagatc gaatgaagct ggtcgtataa accgtcgcat gtttgcaatc gattggctac
     1441 agactcgaag ctttgagctg tgcctatgat tctgtattgt cgctcgggtc cggggttctt
     1501 cggcagagtg aagtctattg cgctttgcgt atcatgccgc accccgaaga actccagcag
     1561 acgtgccgct cgcgattcga tgccacacag gcgatcgccg gtcagcagta ctgccttttg
     1621 acgcattgat gaggcgtgag actccgctca cgcggtcaag aattctaaac tgacggtttt
     1681 tgtgcgatca ctaacgaccg cattgccctc gaccgaccga gattggtcaa tacgggggag
     1741 aatgtccggc atattccggt aagaaaccgg ccggcagaat gtggaatcca acaaaatcgc
     1801 cctgtacgca acacttgaaa accggcccgt gtaaacggcg cggtgtattc ctccaaggat
     1861 ggcaagtagc attcccgctg aggtcgcttg tttccgcgga gtttatcttt caaggcgctg
     1921 cggaatgccg ggaaccgatc gttcatgtcg atcaccacac aaactcctcc tggcatcaga
     1981 agacgccata tatcccgcgc acactccgct tctgtattcg ctcgatgaca atagcggatc
     2041 gtgtttacgc cgataacgaa ttggaacgag ttcacaagat cggcttgcgg gactccagtt
     2101 gtcgcggtga gctcgtcaat cagcgt
//
LOCUS       DATMTI010000814         1858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_72295, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000814 DATMTI010000000
VERSION     DATMTI010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72295"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(462..1736)
                     /gene="rnhA"
                     /locus_tag="VN039_12875"
     CDS             complement(462..1736)
                     /gene="rnhA"
                     /locus_tag="VN039_12875"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HWV46890.1"
                     /translation="MIDIFTDGACSGNPGPGGWGVLLRIGDAETELCGGEPATTNNRM
                     ELLAVIEALQSFTGSVEARVYTDSQYVQKGISEWIHSWKQRGWKTAGKESVKNEDLWR
                     RLDALAAKHTLEWHWVRGHNGHPENERADALARAGLEQARRTGRSIDGPFHDPFKESS
                     SSADSPPILDIRHATVYRGDTCVFSDFSFALREGEHAAILGPNGAGKSTLLKLLSGEV
                     HAMARDETRMALFGDERWNVWDVRKQIGIVSHDLQRDYLICAEGLNVVLSGFYASNDT
                     YEYQQFSKTQMTRAYEVMQELGIIGLSGRRFGHLSTGEQRRFLLGRALVHDPPVLVLD
                     EPTSGLDLKACFQYLDLLRAQIAKGKTVLLVTHHLHEIPPEVDRVIFLKEGKILADGF
                     KQTLLTSDQVSRLFDSKITLVQANGWYQALPG"
BASE COUNT          417 a          536 c          488 g          417 t
ORIGIN      
        1 tcttggccga tgctcaagag aggcaacggt agagaaacct cagaaaaggg caatggcttc
       61 cttccgtgtc gtttcagcgg agaattagca gcgccaggtg gcggactgat gaccggttgt
      121 agagaaagag gtgccgggga aatggtcagc ggtgaatagg tcttcgcctc acccagttgt
      181 cgacagcctg gcccgctgtg atccgtatac agatccgact gtccaggctg aggacagatc
      241 caggtttcgg cccatgccgc gctgtaaaag atctgaacta tcgcacaact gctcagaagg
      301 cacagaagag agagcaggcc tgtcctgcct ttggatttcg agagggcttg atacatgcct
      361 ggctcacgag aataatcagc atctgaagcc tagccctcga aacggcgttc gtcaaaagaa
      421 agggcgtttt aggaggggtc gagaggacac ttcgtacaat atcaccccgg caaggcttga
      481 taccaaccgt tcgcctgaac gagggtgatc ttgctgtcga aaagccggct gacctgatcg
      541 ctcgtaagca aagtttgttt gaaaccgtcg gcgagaattt tcccctcttt cagaaaaata
      601 actcgatcga cctcgggagg aatttcatgc agatggtgcg tcacgaggag aacagtcttt
      661 cctttcgcga tttgagcacg cagcagatcg agatactgga agcaggcctt gaggtcgagg
      721 ccacttgtcg gttcatccag caccagcacc ggcgggtcat gcaccagcgc ccggcccaac
      781 agaaaccgtc gctgctcgcc ggtggacaga tggccgaagc ggcggccgga caaacctata
      841 attcccaatt cctgcatcac ctcatacgcc ctggtcatct gagtcttgct gaattgttgg
      901 tattcgtagg tatcgttgct cgcgtagaaa ccagagagca ccacgttcaa cccctcggca
      961 cagatcagat agtctcgttg gagatcgtgg gacacgatgc cgatttgttt ccgcacatcc
     1021 cagacattcc agcgctcatc gccgaagaga gccatccgcg tttcgtctct cgccatcgca
     1081 tgaacttcgc ctgacaacaa cttgagaagc gtcgatttcc ccgctccgtt aggaccaagg
     1141 atggccgcat gctccccctc tcgcaaggca aaggagaaat ccgagaagac gcaggtatcc
     1201 cctcgataga ccgtggcatg tcggatatcg agtatcggag gagaatcggc tgaactgctg
     1261 ctctctttga acggatcgtg aaacggaccg tcgatggacc gaccggtgcg gcgagcctgt
     1321 tcaagaccgg cccgagccaa cgcatccgcc cgctcattct ccgggtgtcc gttgtgccct
     1381 ctgacccaat gccattcgag tgtgtgcttg gcagccaagg catcgagtcg ccgccacaag
     1441 tcctcattct tcaccgactc tttcccggcc gttttccacc ctcgctgctt ccaactgtga
     1501 atccattcgc taatgccctt ctggacatac tgcgaatcgg tgtaaacccg tgcctccacc
     1561 gatcccgtga atgattgtaa cgcctcaatg accgcgagca gctccatccg attgttcgtc
     1621 gtcgccggct ctccgccaca gagttccgtt tcagcatcgc cgattcgcag caagactccc
     1681 cagcctccgg gaccgggatt cccgctacag gcgccatctg taaagatgtc gatcataggt
     1741 ttcttcttct gattcagaag ttcgcgtccg gattgtacta tgtatgcttc attcgagcta
     1801 tagcctctga cagaaagctg accagttgca cctcgttatg atcagatttg ttttttct
//
LOCUS       DATMTI010000815         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_157819, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000815 DATMTI010000000
VERSION     DATMTI010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157819"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VN039_12880"
     CDS             complement(<1..1330)
                     /locus_tag="VN039_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWV46891.1"
                     /translation="MNLSIAASAAMAMWIMGPFAAIVVQAGSLSDDPILRIETGMHSA
                     NINRMATDKRNLVLATVSDDKTVRIWDLATLAPVKTIRVPIGKGKEGKLYAVALKPDG
                     RVVVCGGQTAYGGEPTGTIFVIDVQTGSVLRAIHTLPSAVLHLAYSKDGRYVVASFGH
                     NGIRIYDAATFSLVAEDKDYAGPSYGAEFSPSGTLATTSYDGLVRLYDATFTLRAKSA
                     SDLGKHPYGVMFSPDGSRIAVGFEDIAGINVLSERDLSLLAEHGSAQTKGALANVVWD
                     AGGSTVYAGGIYDCNGINHLIKWDTRQGTSKKIPTTSHTVTDIVPLQMGGVVFSTSHP
                     SIGIVGASDHLIASKDSPLWDYRGIAQQTVLSDDGTIIHFPNPKGGFYRFSILERSLE
                     AHSHVSHAVRRPPVVAGFVSQKLKTAVESLTEQIKSDHSEIVRVTELTPDG"
     gene            complement(1438..>2075)
                     /locus_tag="VN039_12885"
     CDS             complement(1438..>2075)
                     /locus_tag="VN039_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWV46892.1"
                     /translation="LLDDRVTRSQLEARLEKWLQKQVGDQEDAEVFVYYGGHGAPDPN
                     TGQSFLVPYDGDPSFLEITAYPVNRLYKVLGDLPAKRVVVVVDSCFSGSGGRSVIAKG
                     TRPMLISVTDPVIASSNLIVLSAAAGNQISSAYMDKRHGLFTYYFLKGIQGEADANKD
                     SIVYVQELDAYLKSTVPATARRMQVDQTPQLLPPLENLGPKARTSLVELKR"
BASE COUNT          468 a          574 c          546 g          487 t
ORIGIN      
        1 taccatccgg cgtgagctct gtgactctca ctatttccga atgatcgctc tttatctgct
       61 ccgtgaggct ttcaacggcg gttttcaatt tctgactcac gaagcctgcg acaacaggag
      121 gcctccgcac tgcatgtgag acgtgactgt gagcctccaa tgatcgttca aggatggaga
      181 atcggtaaaa ccctcccttt ggattaggaa aatgaatgat cgttccgtcg tcggacagca
      241 ccgtctgttg ggctataccg cgataatccc acagcggaga gtcttttgat gcgataagat
      301 gatcggaggc tcccacaatg ccgatgcttg ggtgagatgt gctgaagacc accccgccca
      361 tctggagcgg aacgatgtcg gtgaccgtat gagaggtggt cgggattttc ttgctcgtgc
      421 cctgacgcgt atcccactta atcagatgat tgatgccgtt acagtcgtag attcctccgg
      481 catacacggt gcttcccccg gcatcccaca cgacattcgc aagagcgcct ttcgtctgtg
      541 ccgatccatg ctcagcgaga agcgataagt ccctttcgga aagaacattg ataccggcga
      601 tgtcttcaaa tcccactgca atccgtgatc cgtccggtga gaacatcact ccgtatggat
      661 gcttgccgag gtcggatgcc gatttggctc gcagcgtaaa tgttgcatca tataatcgca
      721 caagaccatc ataggaggtg gttgccagcg tgcccgaggg ggaaaattcc gcaccgtaac
      781 tcgggccggc gtagtcctta tcctcggcga ctagagaaaa ggtcgcggca tcataaatac
      841 ggatcccatt atggccgaat gacgcgacaa catatcggcc gtccttggag tacgccaaat
      901 gcaaaaccgc gctgggtaag gtgtgaattg cacgaagcac agagcctgtc tgcacatcta
      961 tcacaaagat ggtaccggtc ggctcgcctc cataagccgt ctgtcctcca cacaccacca
     1021 ctcgtccatc cggctttagg gcgacggcgt agagcttgcc ttccttccct ttaccgatcg
     1081 gtacccggat tgttttgacc ggtgccagcg tggccaggtc ccagatacga accgttttgt
     1141 cgtcggacac ggtagcaagg acgagattgc gtttgtctgt cgccattcga ttgatgttgg
     1201 cgctgtgcat gccggtttca atacgaagaa tgggatcgtc cgaaagtgat ccggcctgga
     1261 cgacaatggc cgcgaaaggc cccatgatcc acatggccat ggctgcggat gcggctatgc
     1321 tcaggttcat cgttgaccga aatatctcaa tcattcgggt atgaggaccg agtgcgtggc
     1381 gcatttccaa gacctctcat cggaggcgaa acgatgccga tgcccggttc actccgatca
     1441 ccgtttgagc tctacaagac tggtccttgc tttcgggcct aaattctcta atggggggag
     1501 cagttgcggt gtttgatcga cctgcatgcg tcttgccgta gcgggcactg tggatttcaa
     1561 gtaggcgtcc agttcttgga cgtatacgat ggaatccttg ttggcgtctg cttctccctg
     1621 tattcccttc aggaaataat aggtaaacag accatgtcgt ttgtccatgt aagcggaact
     1681 gatttgattg ccggcggcgg cgctgagcac aataagattg gaactggcga tcaccggatc
     1741 ggtgacggat atcagcatcg gccgcgtacc cttcgcgatg acggatcgcc ctcccgatcc
     1801 agagaagcaa ctatccacga cgaccacgac ccgttttgcc ggcagatcac ctaagacttt
     1861 atacagacga ttcaccggat acgctgtaat ctctaagaac gagggatcac catcatatgg
     1921 aaccagaaag gattgacccg tgttaggatc cggcgcgcca tgcccgccat agtacacgaa
     1981 tacttcagca tcttcctggt cacccacttg cttctgcagc catttctcca aacgagcctc
     2041 cagctgactc cgggtgaccc tatcatcaag aagca
//
LOCUS       DATMTI010000816         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_751709, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000816 DATMTI010000000
VERSION     DATMTI010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751709"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VN039_12890"
     CDS             <1..386
                     /locus_tag="VN039_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46893.1"
                     /translation="EKKQAWEEVAKSRGVDRGELTRERVEEYFHLPLGQRMLKAKLTR
                     FVGIGIALSQSFARWRMWEVLTKELHLWPMGKRVPLEDRPSGWSRTICPIVGGMSHEY
                     PVEWINANPEMSELVERRESGYDWD"
     gene            428..787
                     /locus_tag="VN039_12895"
     CDS             428..787
                     /locus_tag="VN039_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46894.1"
                     /translation="MNIKATIGSHAPAERANEFVQVIDALIEEENNRTRAAAEKDGPV
                     FVQPNISFAVEAEKVGSVKRQMREAAHLRERTARLTAETAHPSGKKDVPDEIELTYIL
                     TDRITRTTGDETETATE"
     gene            854..1003
                     /locus_tag="VN039_12900"
     CDS             854..1003
                     /locus_tag="VN039_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46895.1"
                     /translation="MGVWAIIGIGFDFALFAAIGVLLFATFAQMDKEAMEEEWEEEER
                     NANAE"
     gene            987..1271
                     /locus_tag="VN039_12905"
     CDS             987..1271
                     /locus_tag="VN039_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46896.1"
                     /translation="MRTRSKAVLPLPRYVMPENVEIGDTIRVAYPAVNGIKKTMEGTV
                     AARDYSGSVRMLMTAQNAVILVYGPSVPHRTVTLLARRNAEQTQLEGIEL"
     gene            1268..1474
                     /locus_tag="VN039_12910"
     CDS             1268..1474
                     /locus_tag="VN039_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46897.1"
                     /translation="MNDEQAEEKWKEMLGGLNRELERRLEADAKREARIVVGLVIASA
                     AIAGLAVIGLVTVIVDVVRAVTGR"
     gene            1610..1849
                     /locus_tag="VN039_12915"
     CDS             1610..1849
                     /locus_tag="VN039_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46898.1"
                     /translation="MNETQAAIKRTITVDGFIVFQKVKEESYDRIQEVHRTVDIIDAT
                     NKADTLCRAYPGIEFILYSGLTGKWYSYFKPIKED"
     gene            1849..2055
                     /locus_tag="VN039_12920"
     CDS             1849..2055
                     /locus_tag="VN039_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46899.1"
                     /translation="MLHTDYIAKLARESQEPNFRPWNIDTHAHARPLGIKKGEKLFAV
                     TYTHEPEVTHIINGRELETIGRFI"
     gene            2055..2216
                     /locus_tag="VN039_12925"
     CDS             2055..2216
                     /locus_tag="VN039_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46900.1"
                     /translation="MARHDEGFYCDVKFSSANGEKHICSLAPEHWRQAKAHTCYCGQV
                     MDDTMRRLS"
     gene            2395..2718
                     /locus_tag="VN039_12930"
     CDS             2395..2718
                     /locus_tag="VN039_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46901.1"
                     /translation="MEKSAEEWEADIKRYAELRDDATRVRNWQDAAWWNSKRADAARA
                     WIMARDRETKMKESGLVQCDARESVENGDIVQCQKYQGHCGNHVMHMLTGKLQNGRPY
                     FLYLE"
BASE COUNT          759 a          792 c          854 g          453 t
ORIGIN      
        1 atgagaagaa acaagcgtgg gaggaagtag cgaagtctcg cggtgttgac cggggcgaac
       61 tgacacgtga gagggtggaa gaatacttcc atcttccgct cggacaacga atgttgaaag
      121 cgaagctaac gcggttcgtg ggaatcggaa tcgcgctgtc acaatcgttc gcacgctggc
      181 gcatgtggga agtgttgacg aaggagctac atctgtggcc gatggggaag cgcgtaccgt
      241 tggaggatcg tccttcggga tggtcccgaa cgatctgccc catcgtcggc ggcatgtcgc
      301 atgaataccc tgtggaatgg attaacgcta acccggagat gtcggaactt gtcgagcgcc
      361 gcgaaagcgg gtacgattgg gattgacaac ggcaacacca cacccgactt acaaaggaga
      421 aacaatcgtg aacatcaaag ccaccatcgg cagccacgcc ccggccgagc gcgcgaacga
      481 attcgtgcag gtcatcgatg cgctgatcga ggaagagaac aaccgcacga gggcagccgc
      541 cgagaaggac ggccccgtct tcgtgcagcc gaacatttcg ttcgctgtcg aggctgagaa
      601 ggtcggcagc gtcaagcgac agatgcgcga ggctgcgcac ctccgcgagc gcactgcccg
      661 gctcacggct gagaccgcgc acccgtccgg caagaaggat gtgcccgacg agatcgaact
      721 cacctacatc ctcacggacc ggatcacccg caccacgggc gacgagaccg agaccgcgac
      781 cgagtaacac accgcaccat cgaaaaggcc gggcatcagc cccggccttt tcgcgtatat
      841 aaggaggaat gcaatgggag tatgggccat tatcggaatc ggattcgatt tcgccttgtt
      901 cgctgcaatc ggagtgttgc tcttcgcaac gttcgcgcag atggacaagg aagcgatgga
      961 agaggaatgg gaagaggaag agcgcaatgc gaacgcggag taaggctgtc ctccctctcc
     1021 cgcgctacgt catgccggaa aacgtcgaga tcggagacac cattcgtgtc gcatacccgg
     1081 ccgtcaacgg catcaagaaa acgatggagg gcaccgtagc ggcacgagac tatagcggca
     1141 gcgtccgcat gctcatgact gcgcaaaacg ctgtgatcct cgtctacggg ccgagcgtgc
     1201 cccaccgcac cgtaaccttg ctggcgcggc gcaatgccga acaaacacaa ctcgaaggga
     1261 tcgaactgtg aacgacgagc aagccgaaga gaagtggaaa gagatgctag ggggtctgaa
     1321 ccgcgagcta gaaagacggt tggaagccga cgcgaagcgg gaagctcgga tcgtggtcgg
     1381 gctcgttatc gcatcggccg ctattgcagg actcgcagtg atcgggctcg tgactgtcat
     1441 tgtcgatgtc gtccgagctg tgacggggag gtgacatgat gcggaagata cctcgtgact
     1501 ttatcctcat gatgcgattg atcggacgtg caaagcacgc cggaaccatc gatgtcattg
     1561 acgctggcaa gctcttgcga acggcagtcg gaatgtatag ggtggaggga tgaacgagac
     1621 acaagcagcg atcaaaagga ccatcacagt agacgggttt atcgtcttcc aaaaggtcaa
     1681 ggaagagtca tacgaccgaa tccaagaagt gcaccgaacc gtcgacatca tcgacgcgac
     1741 aaacaaggcc gacacgctgt gccgcgcata cccgggaata gaattcatcc tctactccgg
     1801 gctcaccggc aagtggtaca gctacttcaa accgatcaag gaagactaat gctgcacacc
     1861 gactacattg ccaagctcgc acgcgaatcg caagagccga acttccgacc gtggaacatc
     1921 gacacacacg cccacgcccg cccgctcgga atcaagaaag gcgaaaagct cttcgccgtc
     1981 acctacacac acgaacccga agtaacccac atcatcaacg ggcgcgaact ggaaaccatc
     2041 gggaggttca tctaatggca cggcacgacg aagggtttta ctgcgacgtc aagttttcca
     2101 gcgcgaacgg cgaaaagcac atctgctccc ttgcgccgga acactggcga caggccaagg
     2161 cgcatacgtg ctattgcgga caagtcatgg acgacaccat gcggagactg tcatgagacc
     2221 cccgcgccga tggaaccgcg aacgcgtcgg cttccacacc ctcatgtgca cgaacggcat
     2281 gcacaaacgc tgcacagggc gtgcacggct ctcaggcacc acgtgtctat gcgactgcca
     2341 cccgtataac tggcggcgcg gcttcctcta ctacaaagcg aaaggacgtg tctaatggaa
     2401 aagagcgcgg aagagtggga agcggacatc aagcggtacg cggaactccg cgacgacgct
     2461 acccgcgtaa ggaattggca agatgcggca tggtggaaca gcaagcgcgc agacgcagcg
     2521 cgagcatgga tcatggcgag agatcgggaa acgaaaatga aagagagcgg actagtacag
     2581 tgcgacgcga gggaatccgt agagaacggc gacattgtcc agtgtcagaa ataccaggga
     2641 cactgcggga atcacgtcat gcacatgctc accggcaagc tgcaaaacgg tagaccgtac
     2701 ttcctatacc tcgaatgatg aacgaatgcg ggaccgaact tgtaaccggt cccgcatccg
     2761 tgtatcgtgg aagtaacaag cgaaagggaa cgcacatgga aaagcgcagc attggaatcg
     2821 tcagggtcga aacacgcgac gatccgaaca tccatcaa
//
LOCUS       DATMTI010000817         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_540228, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000817 DATMTI010000000
VERSION     DATMTI010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540228"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VN039_12935"
     CDS             complement(<1..593)
                     /locus_tag="VN039_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HWV46902.1"
                     /translation="MSVFKNLNTARKLMLAFGTVGVIMAGLGYFGINTVSTVNDMVAI
                     LYERELKGLSAIKDVNLAITAISRLTRAAIISTDQKEKEQFSKTIVQLKQQFQDKLSE
                     FQKLTITDEAKTISAELARIFPPYMELMEEAVRLTLRDDNVNAIKKVHDAISDAQQVM
                     SKASELAVMKEMQAERVYKESNVLYERSRNIMEGLTIV"
     gene            complement(768..>2230)
                     /locus_tag="VN039_12940"
     CDS             complement(768..>2230)
                     /locus_tag="VN039_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWV46903.1"
                     /translation="ESAYAASADYQAFWAKLNRGEFDTGVYRRVGKGGKEIWIQASYN
                     PILDANGKVYKVVKFATDITGQKQAQNEVEKLIHAAAMGNLSDRINTGRFQGSAKDLV
                     SSFNQLLDAVATPLHEAQGVLTAMASGDLTKQMTGAYQGEFEQMKMSLNTAITQLTQT
                     VSLVRGAVEAVTAGSEEISKGSDDLAQRTSEQAGALEETSASMEEMTSTVKQNADNSK
                     QANQLAIAARDIANKGGAVTTKAVDAMGEINKSSKKIADIITVIDEIAFQTNLLALNA
                     AVEAARAGEHGRGFAVVAAEVRNLAQRSATAAKEIKGLINESIQRVTDGTELVNQSGK
                     TLEEIVGSVKRVTDIIAEISAASQEQASGIDQVNKAIMQMDEGTQQNAALVEETASAS
                     QSMKEQAKELLEQMNMFKTGQGAVTMRSTVTARGDRPHGMGQPKAVSITGSGGSMGEA
                     KKPVRTGSQPAAAAPTALAVGNVNDRRGAGDDFEEF"
BASE COUNT          410 a          673 c          562 g          585 t
ORIGIN      
        1 acaatggtga gcccctccat aatattgcgg gagcgttcat agaggacatt gctttccttg
       61 tagacccttt cagcctgcat ctccttcatg accgccagtt cggatgcttt actcatcact
      121 tgctgcgcat cggagatggc atcatgcacc ttcttaatcg cattgacgtt gtcatcccgc
      181 aaggtcaacc tgaccgcttc ctccatcaac tccatgtacg gagggaagat tcgcgctaac
      241 tccgcagaaa tggttttcgc ttcatctgtg atagtcaact tctgaaactc ggacaacttg
      301 tcttgaaact gctgcttgag ttgaacgatc gttttgctga actgttcctt ttccttctga
      361 tcagtggaaa tgatcgccgc gcgtgtcaac cggctgatgg cagttatggc aagattgaca
      421 tccttgatcg ccgacagccc cttgagttcc cgttcataga gaatggcaac catatcgttc
      481 accgtcgaca ccgtgttgat tccgaagtac ccgaggcccg ccatgatgac ccctaccgtg
      541 ccgaacgcca acatcagctt cctcgctgta ttcaaattct tgaacacgct catgtatttt
      601 tcttacctcc tacactgcta tttacgtaaa tataagcgct agtcctgtca tgggcggacc
      661 gaatgtcgtt ttgagaccga cgacgagtct tgtactcggg aacctgtcgt tcctgagaac
      721 aacactccca tctgatatcc gtcgtgatcg ttcctgggaa gggtttccta gaattcttcg
      781 aaatcgtcgc cggcgccacg acgatcgttg acgttgccga ccgcaagtgc ggtcggtgcc
      841 gccgcagcag gctgcgagcc ggtccgcacc ggctttttcg cctcgcccat ggatccgcct
      901 gacccagtga tcgagacagc cttgggttgt cccatcccat gcggacgatc tcctcgggca
      961 gtgaccgtgg atctcatcgt gaccgcgcct tgaccggtct tgaacatgtt catctgctcc
     1021 agcaactcct tagcctgttc cttcatcgat tggctggctg aagcggtttc ttcgaccagg
     1081 gcggcattct gctgggtgcc ttcatccatc tgcataatgg ccttgttcac ctgatcgatc
     1141 ccgctggcct gctcctgcga cgcagcgctg atttcggcaa tgatgtcggt gacacgtttg
     1201 actgatccga cgatttcctc aagcgtcttc ccggactgat tgaccaactc ggtgccgtcc
     1261 gtgacacgct ggatcgattc gttgatcaag cccttgatct cttttgccgc agttgcggaa
     1321 cgctgggcta aattccgcac ttccgccgcc accacggcaa accctcggcc atgctccccg
     1381 gcccgcgccg cttccaccgc cgcattcaac gccaaaaggt tggtctggaa ggcgatctcg
     1441 tcgatgacgg tgatgatatc ggcgattttc ttgctgctct tgttaatctc tcccatcgcg
     1501 tcgaccgcct tggtggtgac cgctcccccc ttgttggcaa tatctcgggc ggcgatggcc
     1561 aactgattcg cttgtttgga gttgtcggcg ttctgtttga ccgtggacgt catttcctcc
     1621 attgacgcgg aggtttcttc caaggcgccg gcctgttcgc tcgtccgttg cgcgaggtca
     1681 tcgctgcctt tactgatttc ctccgatcca gccgtgacgg cttcgaccgc tccacgcacg
     1741 agcgacaccg tttgagtcaa ttgtgtgatc gccgtattga ggctcatctt catctgctcg
     1801 aactcgcctt gataggcacc cgtcatctgc ttggtcaggt caccggaggc catcgccgtc
     1861 aagacgccct gtgcttcatg cagcggcgta gcgacggcat caagcaactg attgaagctc
     1921 gacacaagat ccttggctga gccctggaac cgtccggtat tgatccgatc ggagagattg
     1981 cccatggcgg cagcgtggat gagcttttcg acctcgttct gcgcctgttt ttgaccggtg
     2041 atgtcggtgg cgaatttgac gactttgtac actttcccat tggcatccag gatggggttg
     2101 taggacgcct gaatccaaat ttcttttcct cccttcccaa cccggcgata gacgccagta
     2161 tcgaactcac cccgattgag cttggcccag aaagcctgat agtcggccga tgccgcatag
     2221 gccgattcgg
//
LOCUS       DATMTI010000818         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_540291, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000818 DATMTI010000000
VERSION     DATMTI010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540291"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..2200
                     /locus_tag="VN039_12945"
     CDS             143..2200
                     /locus_tag="VN039_12945"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWV46904.1"
                     /translation="MSDSAIITFALIAAVAGIAYGLYLAMWVFRLDAGNAKMQEIAKA
                     IQEGAGAYMNRQYKTVGYVAAGLFVILAAAGAVSDKFGLITALGFLVGAGASAIAGYV
                     GMIIAVRANVRTAQAAHKGMNAALIVAFRGGAVTGLLLIGLGLLAITGFYTIAESMAG
                     QEKAIHALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTTVAAMVLAFTMFKGATAPILYPLALGSVTIFAT
                     IIGILFVKVTPGGEIMSALYKGLFMAGGIAAVAFLPITFMIMGGVGGVSGFSYYIAAL
                     IGLAVTLALVFITDYYTSKSYEPVKYIAKASETGHATNIIAGLAVGMQSTAAPVVVIA
                     AAILGSFWICGGAESGGLYGVAVAAVSMLSMAGIVVAIDAFGPITDNAGGIAEMSHLG
                     KEVRDITDPLDAVGNTTKAVTKGYAIGSAGLAAVVLFAEYSREVAAHNPALAAFDLSN
                     PKVLVGLFLGGMLPFIFGAMCMKAVGEAGGLIVEEVRRQFRTIKGIMEGTGKPEYGTC
                     VDIVTQAAIQKMMIPGLIPVVSPVIVGVVLGPQALGGVLVGSIVTGLFVAISMTSGGG
                     AWDNAKKFIEEQGKKGSETHKAAVTGDTVGDPYKDTAGPAVNPMIKVINIVALLIISL
                     IVG"
     gene            2425..>2562
                     /locus_tag="VN039_12950"
     CDS             2425..>2562
                     /locus_tag="VN039_12950"
                     /inference="COORDINATES: protein motif:HMM:NF017455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031386 - protein tag activity [Evidence
                     IEA];
                     GO_function: GO:0070628 - proteasome binding [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HWV46905.1"
                     /translation="MEKQERKQETRREPQGKEEVKANPKVVETGKKIKEDVDKLVDEI
                     DD"
BASE COUNT          533 a          610 c          812 g          607 t
ORIGIN      
        1 tgacgcagct tttcgggtgg ttagcttctc attcgactcc acccgccttg gatttaccca
       61 ctcgcgcatg tactttggat gggttgaagc cacggagagc cgtgtgacat aaggagccgt
      121 ttaaccagca ggaggatgtc ccgtgagtga ctcagcgatt atcacgtttg ccctcatcgc
      181 agccgtggct ggtatcgcct atgggctgta tctcgcgatg tgggtgttca ggctcgacgc
      241 cggcaatgcc aaaatgcagg agatcgcaaa agccattcaa gaaggtgccg gcgcttacat
      301 gaaccgacag tacaagaccg tcggttatgt cgctgcgggc ttgttcgtca ttctggcagc
      361 ggctggagcc gtgtcggata aatttgggct gatcactgcc ctcggctttt tggtcggcgc
      421 gggggcctcg gccattgccg gctacgtggg catgatcatc gcggttcgcg ccaacgtgcg
      481 gacagctcaa gctgcacata aaggcatgaa tgccgcactg atcgtggcct tccgcggtgg
      541 agcggtaacg ggcttgctgt tgatcggtct cggactactg gctattaccg ggttttacac
      601 tattgcagaa tcaatggctg gacaggagaa agccattcat gcgttgctca gtcttggatt
      661 cggcggcagt ctgatctccg tcttcgcacg cgtcggtggc ggcatctaca cgaaggcagc
      721 cgacgtcggt gccgacttgg tcggcaaagt tgaagcgggg attccggaag atgatccgcg
      781 gaatccggcc gtgatcgcag ataacgtggg tgacaatgtg ggtgattgcg ccggaatggc
      841 ggcagatctg tttgaaacct atgccgtgac gaccgttgcc gccatggtgt tggccttcac
      901 gatgttcaag ggggcgactg ccccgatcct ctatccgttg gctttgggca gtgtcacgat
      961 ctttgccacg atcatcggta ttctctttgt caaggtcact ccgggcggcg aaatcatgtc
     1021 ggcactgtat aaggggttat tcatggccgg tgggattgcc gccgtggcat ttttaccgat
     1081 taccttcatg attatgggtg gagtaggagg agtcagcggc ttcagctact atatcgcggc
     1141 gttgatcggc ttggccgtca cattggcgct cgtgttcatt accgattatt acacgtcaaa
     1201 gagctatgag ccggtgaagt acattgcaaa agctagcgaa accggccatg caacgaatat
     1261 catcgcaggt ctggcagtgg gcatgcagtc gacggcagcg ccggtcgtgg tgattgcggc
     1321 ggcaatcctg ggcagcttct ggatctgcgg cggggcggag tcaggcggac tgtatggtgt
     1381 agcagtggca gcggtgtcca tgctctcgat ggcagggatc gtcgtggcga tcgatgcctt
     1441 cggtccgatc accgataatg ccggtggcat cgccgaaatg tcgcatttgg gcaaggaagt
     1501 ccgagacatc acggatccat tggatgcggt cggcaatacg accaaggcgg tcacaaaggg
     1561 ctatgcgatc gggtccgctg gtctggcagc agtggtgtta tttgccgaat actcacgaga
     1621 ggtcgcagcg cataatccgg ctctagctgc gttcgatcta tcaaatccaa aagtcttggt
     1681 tggtctcttt ctcggtggca tgcttccctt tatattcggc gcaatgtgta tgaaggccgt
     1741 cggtgaagcg ggtgggttga ttgtggaaga agtgcggcgt caattcagaa cgatcaaggg
     1801 cattatggag gggaccggta agccggaata tgggacttgc gtggatatcg tgacccaggc
     1861 ggcgatccaa aagatgatga tccccggttt gattcctgtg gtttcgccgg tgatcgtcgg
     1921 tgtggtcctt gggccacaag cgctcggcgg tgtgttggtc ggcagcatcg tgaccggttt
     1981 gttcgtcgca atttcgatga cgagtggcgg cggcgcgtgg gataacgcca agaagttcat
     2041 cgaagagcag ggcaaaaagg ggagcgagac acacaaggcg gcggttaccg gcgatacagt
     2101 cggtgatccg tacaaggata cggcgggtcc tgccgtgaat ccgatgatca aggtgatcaa
     2161 cattgtggca ttgcttatta tctcgctcat tgtagggtag catctctcgt acctctcgaa
     2221 gtttgttcgt gtcgtgaaaa tggttgcttg acgctttctc atgatcgagc ggtgttcctc
     2281 tcccgattcc tgaaggcatg agacgatcgg ctcttttctc ggcaagagcc gcctcatggt
     2341 tcctacgtta gcttgacggt tttgcgaatg ctggagtaag gtaacttatg gtgaaactgt
     2401 cgttgattac cgggaggtgc tcggatggaa aaacaagagc gaaagcagga aaccagacga
     2461 gagccgcaag gaaaggaaga agtcaaggcg aatcccaagg ttgtcgaaac cggcaagaag
     2521 atcaaggaag acgtcgataa gctggtcgat gaaattgacg at
//
LOCUS       DATMTI010000819         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_581889, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000819 DATMTI010000000
VERSION     DATMTI010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581889"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1561)
                     /locus_tag="VN039_12955"
     CDS             complement(<1..>1561)
                     /locus_tag="VN039_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HWV46906.1"
                     /translation="YSERYLPTGKSLHFRTTSRKDNPEGVSVLRNAYRPWYFLKRIEE
                     IEAIGVERDLAGIPIATVPAEMLSENANDADKKMIASIIKLVQNVRRDEQEGIVWPQA
                     YDQNGKELYDFKLLTSGGTRQFPTNEIITRYESRIAMTVLADFLMLGNDSSTGSYALS
                     TSKASMFQSALETWLNVIENVINEQAVPRLFRFNGIKGPYPKFRHDIVQKPSLTDLAT
                     FLSSMAGAGAQLFPDTGLENHLREFAELPIREENRGNEATEDKIIEQQLDTMLAESES
                     QEQIADRTGKDANAEGVVPIGQGSIAPGRFAPTGGAAAATGATTTGAGGSTAAAATGP
                     GGKKPGSKGKAPTKSTGPVKSKQPPTKRRATISVRASQNQPAKSTGGKKNTSVAKHNA
                     NYPHTAKSMLIKQLAHDFPVKAMGWLDHAVVEGPIAVPVNDVNTKDRDEWAATHEPAK
                     IDHFKAKMASGQMKPILMVQPPKGKKIIVDGHHRYTAGEQLGHTHVLAYVAHVPKKSG
                     PWDEMHSSQFVR"
BASE COUNT          266 a          463 c          459 g          373 t
ORIGIN      
        1 cgcacgaact gagaagaatg catctcgtcc cacggcccgg atttcttcgg aacatgcgcc
       61 acgtaagcga ggacgtgagt atgcccgagt tgttctcctg ccgtgtaccg gtgatgcccg
      121 tccacgatta ttttcttgcc cttgggcggc tgaaccatca ggatcggctt catctggccg
      181 gaggccatct tggccttgaa atgatctatc tttgcaggct cgtgagtagc cgcccattca
      241 tcgcggtcct tggtgttcac gtcattgacg ggaaccgcga tcggaccttc cactaccgcg
      301 tgatcgagcc agcccatcgc cttgaccggg aaatcatggg ccagttgctt tatcagcatc
      361 gacttcgccg tatgcgggta gttcgcgttg tgcttggcca ccgaggtgtt ctttttcccg
      421 cctgtggact tcgcaggctg attctgggaa gcacgcaccg agattgttgc gcggcgcttg
      481 gtcggtggct gcttgctctt gactggccct gtggacttcg taggagcctt gcccttgctc
      541 cccggcttct tcccacccgg accggtcgct gcggctgctg tagagcctcc tgcgcccgtt
      601 gttgtggcac cagtggccgc cgctgctcca ccagtgggtg cgaacctccc aggggcgatg
      661 ctgccctgac cgatgggaac gacgccttcc gcgttggcgt cctttccggt ccgatcggcg
      721 atctgttcct gagactcaga ttccgcaagc atcgtgtcga gctgctgttc gatgatttta
      781 tcttcagtgg cttcgttgcc ccggttctct tcccgaatcg gcaactcggc gaactcacga
      841 agatgattct ccagaccggt gtccggaaaa agctgcgctc ccgcaccagc catggaggac
      901 aggaacgtag ccaggtctgt caggctcggc ttctgcacga tgtcatggcg gaacttgggg
      961 tacggaccct tgatcccgtt gaaccggaac aaccggggta cggcctgttc gttgatgacg
     1021 ttctcgatga cgttcagcca agtttccaaa gccgactgga acatgctggc cttggacgtg
     1081 gacagcgcgt aagaacccgt cgagctgtcg ttcccgagca tgaggaagtc agccagcacc
     1141 gtcatcgcga tacgggattc gtaccgagtg atgatttcgt tcgtggggaa ctgccgagtg
     1201 ccgccagaag tcagcagctt gaagtcgtac agttccttcc cgttctggtc gtacgcctgc
     1261 ggccagacga tgccttcctg ttcatcccgc cgcacgttct gcaccagctt gatgatgctg
     1321 gcaatcatct tcttgtcggc gtcgttggca ttctccgaca gcatttccgc cggaaccgtg
     1381 gcgatcggga taccggccag gtcgcgctcg acgccgatgg cttcgatctc ttcaatgcgc
     1441 ttgaggaagt accacggtcg gtaagcgttg cgaagaacag aaacgccttc agggttgtcc
     1501 ttgcgcgaag tggttctgaa gtgaagagac tttccggtgg gcaggtaccg ctccgagtag
     1561 t
//
LOCUS       DATMTI010000820         4345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_540368, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000820 DATMTI010000000
VERSION     DATMTI010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540368"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VN039_12960"
     CDS             <1..1022
                     /locus_tag="VN039_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HWV46907.1"
                     /translation="LELGLQGADSIKDRRIPTFSRGELPHFAGINTFIKAPYVEDVRK
                     CGQYDVAILGAPFDGGTTYRSGTRFGPQGIRKISALYGTYSFELGVDLRESVSICDVG
                     DVFTIPGNIEKTFDQVSKGVGHVYASGAFPVVLGGDHSLGFATVRGVAQNMNGKKLGI
                     LHFDRHVDTQETDLDERMHTTPWFHATNIPNVPAKNLVQIGIGGWQAPRPGVKVGRER
                     QTTIMTVTDCVEMGIENAAKQALEVAFDGVDAVWLSFDVDCLDAAFVPGTGWPEPGGF
                     LPREVLKFLQIIADTKPLAGMEIVECSPPYDAAEITSLLATRVICDVLACQVRSGNLL
                     NRKKR"
     gene            1421..1648
                     /locus_tag="VN039_12965"
     CDS             1421..1648
                     /locus_tag="VN039_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46908.1"
                     /translation="MPMRKKTNIMSDNSPTVRSRREFKPKDGKVENQGRVIRPMKQTW
                     QLFTDSLTMFTDDFMEDRQQPSHQKRKSSFA"
     gene            1857..3506
                     /locus_tag="VN039_12970"
     CDS             1857..3506
                     /locus_tag="VN039_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWV46909.1"
                     /translation="MKILIGLLVVVVLLVGVVLALPFLVDLNKYQDQYKPLIEDALNR
                     KVQLQDIRLTIWPRIGARVAGFAVLDDPSFGSTPFASLSSLDVGVKLMPLLSGKVEVE
                     EITLHQPVITVIKNKNGVLNASTIGRKGVAVPEKPSRAPIPSTEGPLKILALLAVDRV
                     SIDGGQLTYRDLSAAKPTEYVLQDMELLLQDVRLGRTPRLHFLSLVQPFNLPVKLDGE
                     FGPLRETMDIEAINFELGMGKTDFTITGNAAGNDANIYITSPVITTANLPVSLPLKKP
                     VDIKNFQIAAEVKGQGIKLNTLSFQLFDGQMKGQGKLIAGPDAPPFKGGVTVQGVQLG
                     PALDAVAETPISVSGTAGADLSLQGIGFSMPDLTTALEGTGHMTVKDGKIEGVNLLQE
                     VVSALKVAGISLDNAKATAFSTIETDLAIKQGIINVQRLLMDSHDFQATGGGTIGFDQ
                     RLNLLVNLNLSQDLSREIAAASPVVKLAVKDGRLSLPLTITGTAQAPTYGVDLKGVTG
                     KVHQQVRKKVEEAVDGLIKGTKTPEDLKKEGQELLKGLFGR"
     gene            3590..>4345
                     /locus_tag="VN039_12975"
     CDS             3590..>4345
                     /locus_tag="VN039_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWV46910.1"
                     /translation="MTVVDTLTQYAVQYGLQAAVAFGILIAGSMASRWAGNFAQQTLE
                     RQTLEPPVRLLLVRIVKIVVMLFTAMIALQTLGVPIAPLIAGLGVAGVGIGLALQGVL
                     SNVMAGLSIIFSKPYKVGEHISLLGVHGDVVVIDIFTTTLMHADRSRVIIPNRKIVGE
                     ILHNFGTIRQLHLTIPVSPNANLDEAIVQVRDVLDRHPSVMKDPVPSVGVSSLGESSL
                     TVAIAVEPWTAAANYGATQGELNKLILQRFQERG"
BASE COUNT          961 a         1197 c         1240 g          947 t
ORIGIN      
        1 gccttgaatt gggcctgcag ggcgccgatt ccatcaagga tcgccggatc cccacgttca
       61 gccggggaga actgccgcat tttgccggca tcaacacctt tatcaaggct ccctatgtcg
      121 aagatgtccg taagtgcggc caatacgacg tggccattct cggcgcgccg ttcgatggcg
      181 gaacgacgta ccgctccggc accaggttcg ggccccaagg gattcgcaag atctccgcac
      241 tgtacggaac ctatagtttc gagctcggcg tggatctgcg ggagtccgtg tcgatctgcg
      301 atgtcggtga cgtgtttacg attcccggca acatcgagaa gaccttcgat caagtcagca
      361 aaggcgtggg gcatgtctac gccagcggcg cgtttcccgt ggtactcggc ggagaccatt
      421 ccctgggttt tgccaccgtg cggggcgtgg ctcagaatat gaacggcaag aaactcggca
      481 tccttcactt cgaccggcat gtggacacgc aggagaccga tctcgatgaa cgcatgcaca
      541 ccacgccctg gtttcacgcg acgaatatcc ctaacgtgcc ggccaagaat cttgtgcaga
      601 tcggcatcgg cggctggcag gcccccagac cgggcgtgaa ggtcggccgt gaacgccaaa
      661 ccacgatcat gaccgtgacc gattgcgtgg aaatgggaat cgagaatgcc gcgaagcagg
      721 cgctcgaagt ggcgtttgat ggtgtggatg cggtgtggtt gagtttcgac gtcgattgtt
      781 tggacgcggc cttcgtgccg ggcaccggct ggccggagcc cggagggttt ctgccgcgtg
      841 aagtgctgaa gtttctccaa atcatcgcgg acacgaagcc actggccgga atggagatcg
      901 tcgagtgctc gccgccctac gacgcggcgg agattaccag cctcctggct acgcgtgtca
      961 tctgcgacgt cctggcttgc caggtacggt ccggtaacct gcttaacagg aagaaacgct
     1021 gagatgctct gattcagaaa tgacgaaaca gctgctcggg ggcttgcatt catgcaagcc
     1081 cctaggcact tcgtcagtgg cgagctttgc tcctgcgcgt tgatggaaaa cggattctcc
     1141 tgctgcaagg cttcctcaaa aaagcgatcg atcaggcacg gttttgcaat cgtgtgcgca
     1201 ttaacatatg tcgctcatcc gcgatcgtta gattcagcca gtccacggct cagtcgaaag
     1261 cccggtcgca gtggtgcgaa tccttcctgg tccattgagc tcggcgcaac acaccgcctg
     1321 ggcggcgtga actcgttatt cgctttgcct ctccgttgac agatgacgca ccactcttca
     1381 tcatgggcat ggaatattgt cattgaaaaa gaggcttacg atgccgatgc ggaagaagac
     1441 aaacattatg agtgacaaca gcccgacggt tagaagtcga cgggaattta aaccgaagga
     1501 tggcaaagtc gagaatcaag gacgagtcat tcgtccaatg aagcagacct ggcagctgtt
     1561 caccgatagt ctgaccatgt ttaccgacga ctttatggaa gatcggcagc agccgtcaca
     1621 ccagaaacga aagtcctcct tcgcatgaag gtggtgctgg ataccaacat ctgcatctac
     1681 ctcatcaaac aacagcctcc cactataccg agaagttcaa tcgctacaca gatcactagg
     1741 aattgcagcg aagtcaaata ttgtagagtg ccctcacgat tattgtgtgc agccctcttc
     1801 gtttgactca ccgagactcc atttatatac tccattttta actggaggcg atcgagatga
     1861 aaatcctgat aggcttgctc gtcgtagtcg ttctcctcgt cggcgttgtg ctcgcgcttc
     1921 ccttcctagt cgatctcaac aagtatcaag accagtacaa gcccctcatc gaagatgcgc
     1981 tcaatcgcaa ggttcagcta caagatattc gcttaacgat ctggcccagg atcggtgcac
     2041 gtgtggcagg attcgcggtg ctggacgatc cgtccttcgg ctcaactccc tttgcatcgc
     2101 tctcctcgtt ggatgtcggt gtgaagctga tgccgttgct gagcggcaag gtcgaggtgg
     2161 aggaaatcac gcttcaccag ccggtcatca cggttattaa gaacaaaaac ggagtcctga
     2221 acgcctccac catcggtcgc aaaggcgtgg cggtgccgga gaagccgtct cgcgctccga
     2281 ttccctcgac ggagggaccg ctcaaaattc tcgcgctctt ggccgtggac cgagtgtcga
     2341 tcgacggtgg ccaattgaca tatcgcgatc tgtcggctgc caagccgacg gagtatgtgt
     2401 tgcaggatat ggagttgctc cttcaggacg ttcggctcgg ccgaactcca cgtctacatt
     2461 tcctctcgct cgtgcagccc ttcaatctgc cggtgaaact cgacggtgag ttcggcccgc
     2521 tgagagaaac gatggacatc gaggcgatta atttcgaact gggtatgggg aagaccgact
     2581 ttactattac aggcaatgcc gccgggaatg acgccaatat ctacatcact tcgccggtga
     2641 tcactactgc gaaccttccg gtctcgcttc ccctgaaaaa gccggtcgac attaagaatt
     2701 tccagatcgc ggcggaagtg aagggacaag ggatcaagct gaataccttg tcgttccaac
     2761 tgttcgacgg ccagatgaaa gggcagggca aactgatcgc cggacccgac gcgccgcctt
     2821 tcaagggcgg tgtcacggtt cagggagtgc agctcggtcc ggcgctcgat gccgttgccg
     2881 agacgccgat ctccgtcagc ggaacagccg gtgccgacct ctcgctgcaa ggcataggat
     2941 tctctatgcc ggatctgaca acggcattgg aaggaaccgg gcacatgacg gtgaaggatg
     3001 ggaagattga aggggtgaat cttctgcagg aagtggtctc ggctctcaag gtagccggca
     3061 tttcgctcga caatgccaag gccactgcat tttctacgat cgaaacggat ctcgccatca
     3121 agcaggggat tatcaacgta cagcgtctgt tgatggacag ccatgatttt caggccaccg
     3181 gcggcggtac catcggattc gatcagcgat tgaatctcct cgtgaatctc aacctatccc
     3241 aggatctgag ccgggaaatc gccgccgcgt cacctgttgt gaaacttgcc gtcaaagacg
     3301 gccggttgag tcttccgctc accattaccg gcaccgcgca ggcgccaacg tatggagttg
     3361 atctcaaagg cgtcacagga aaagtgcatc aacaagtacg gaagaaggtg gaagaagcgg
     3421 tcgatggatt gatcaaaggt acgaaaacac cggaggactt gaagaaagag ggacaggagc
     3481 ttctgaaagg actcttcgga cgatagcagg acgcggtgat agccctacct ccccggcctt
     3541 ctcgtacggt tcaagtattc aacgtagcct ctagaaggag acgaccccta tgactgtcgt
     3601 tgatacgctc actcagtatg ccgtgcagta tgggctgcag gcggcggttg ccttcggcat
     3661 cctcatcgcg ggcagcatgg cgtcgcgctg ggccggtaac tttgctcagc agacgctgga
     3721 acggcagacc cttgagcccc cggtgcggct gctcctggtt cgcatcgtca aaatcgtggt
     3781 gatgctgttt accgcgatga tcgccctcca gacgctcggt gtgcccattg cccctctgat
     3841 cgccggcctc ggagtcgcgg gagtgggtat tggtctggcc ttgcagggtg tgttgagcaa
     3901 cgtgatggcc gggctctcga ttatcttcag caagccctat aaggtcggcg agcatatttc
     3961 gttgctcggt gtccacggtg acgtcgtggt gatcgacatc ttcaccacga cgctgatgca
     4021 cgcggatcga tcccgcgtca tcatccccaa tcggaaaatc gtcggagaaa tattgcacaa
     4081 tttcggaact atccgccagc tgcatttgac cattcctgtg tcaccgaacg cgaatctcga
     4141 tgaggccatt gttcaagtcc gggatgtcct tgatcgacac ccatcggtca tgaaagaccc
     4201 ggttccttca gtaggagtgt catctcttgg ggagtcctct ctgacggtgg ctattgccgt
     4261 agaaccttgg acggctgcgg caaattacgg tgcgacgcaa ggcgagttga acaaattgat
     4321 tcttcaacga tttcaagaac gtggg
//
LOCUS       DATMTI010000821         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_708619, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000821 DATMTI010000000
VERSION     DATMTI010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708619"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1378)
                     /gene="nagZ"
                     /locus_tag="VN039_12980"
     CDS             complement(281..1378)
                     /gene="nagZ"
                     /locus_tag="VN039_12980"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HWV46911.1"
                     /translation="MSYDQIGQLFMIGFDGTTVSAELASFLREYKPGGIILFSRNLES
                     IAQIIELTSDLQRYSPHSPLLISIDQEGGRVSRLPKDFTIFPPCEVLGRCRSSELAYA
                     AAATTAKELKAVGINMNMSPVLDVNSNLANPVIGDRAFGTTPDIVSELGLATVGGLQD
                     NRVVACGKHFPGHGDTNSDSHKELPSVPVTRERLERVEFPPFRRAIANGVATMMTAHV
                     LYQALDEDRPATLSPTIIGRFLREELHYDGVVLTDDLEMHAIIDHYGIGDAAIQAIQA
                     GCDMPLICRDRNRIVAALNAVDKAIANGDISAGRLAQSLARIRRLKERFLLPYRPMMI
                     SDAKLVVGCRSHKALLRSINQTRERFSKAEL"
     gene            complement(1402..>2680)
                     /locus_tag="VN039_12985"
     CDS             complement(1402..>2680)
                     /locus_tag="VN039_12985"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWV46912.1"
                     /translation="ILVGLGKKHELGLDHIRQAMGHAAKRVRHAKCGAFTVALPSVVP
                     HGASPIDVGQTMAEGAILGSYQFTTYRSDASSKDVMAMTVLASRKDQLHQLSEGIRRG
                     VATAEATAFVRDLCNHPSNVMTPTRIAQEAKTVAKAAGVNLKILEQKEMEKLGMGALL
                     GVAKGSHEPPKFIILQYRGAKKKDDRPVVLVGKTITFDTGGISLKPAENMEQMKADMT
                     GGAEVLAAMRAAARLKLPLNLISILPAAENMPGGRAMRPGDVVKTLSGKTVEVQNTDA
                     EGRLILSDGLAYATRFKPAALIDIATLTGACVVALGQFAIGMFGTDAALKEAIRKAGL
                     RAGERVWEMPLWEEYFEQLRSDVADMRNIGGRGGGMITAALFLSKFVGDAPWVHLDIA
                     STDWSERERAYIPKGPTGIGTRLLIQFLINRSL"
BASE COUNT          589 a          766 c          725 g          600 t
ORIGIN      
        1 ctccgcaaat acaacgcgat cggattaccc cgatagggcc caagaaaaat atggacggat
       61 ccaacatact cgagcaatga ggccataagg ccctccaagg gacgttcatg atgccacaag
      121 agatgagaag agggcaagat attcagtggg gagaatggag gtttcgcttc ctcacttcat
      181 tgaaatgaga tcttccctcc tgatggaact caacgaatct ccctggagac aatgctcaat
      241 gttgtctagc ctgccatttt gaaaagaaag aagactccgg tcatagctcg gctttcgaaa
      301 atcgttcacg tgtctggttg atggagcgta agagagcctt atggcttcgg cagcccacca
      361 caagtttggc atcagaaatc atcatgggtc gatagggaag aaggaagcgt tcttttaagc
      421 ggcgtatacg tgcgagagac tgagccaatc gtccggcaga gatgtccccg tttgctatgg
      481 ccttgtcaac ggcattcagc gcggctacga ttctattacg gtctctacag atcaatggca
      541 tgtcgcaacc ggcttggatg gcctgtatcg cggcgtcgcc gatgccatag tgatcaatga
      601 tggcatgcat ctcgagatca tcagtcaaca cgacaccatc gtagtgcaac tcttcacgaa
      661 gaaatcttcc aatgatcgtc ggagacaggg ttgccggccg gtcctcatcc aaagcctgat
      721 acaaaacatg ggccgtcatc atcgttgcca cgccgttggc aatcgcgcgc cggaatggag
      781 gaaattcaac gcgctcaagt cgttcacgcg tgaccggcac actgggcaac tccttgtggg
      841 agtccgagtt ggtatcgcca tggccaggaa agtgtttccc acaagccacg acacgattgt
      901 cctggaggcc tcccactgtg gccaacccca attcagacac gatatcagga gtggtgccga
      961 atgctcgatc cccaattacg gggttggcga ggttgctgtt cacatccagc accggagaca
     1021 tattcatgtt gatgccgacg gccttgagct cttttgcggt cgtcgctgct gcagcgtagg
     1081 ccagctcgga ggagcggcat cgtcccagga cttcgcacgg tgggaaaatc gtaaagtctt
     1141 tcggcaaccg tgagacacgt ccgccttctt gatcgataga gataagaagg ggagagtgcg
     1201 gactatagcg ttgaagatcg cttgtcaatt caatgatctg tgcgatcgat tcgagatttc
     1261 tcgaaaacag gatgatgcca ccgggtttat attcccggag gaaggaagcg agttcagccg
     1321 aaacggtggt gccatcgaac cctatcatga agagttggcc gatctggtcg taagacatct
     1381 tcgaaccgcc ttgctctcgc cctacaggga gcgattgatg aggaactgga tcaacagcct
     1441 tgtcccgata ccggtcgggc ctttggggat gtaagcccgc tctcgctcgc tccaatcggt
     1501 gctggcgatg tcaagatgta cccaaggggc gtcgccgacg aacttgctca agaagagagc
     1561 agccgtgatc atgccgcccc cccgtccacc gatgttccgc atgtcggcca catcgctgcg
     1621 cagttgctcg aaatattcct cccacaacgg catttcccat acccgctcgc ctgctcgaag
     1681 gccggccttg cgaattgctt ctttcaacgc cgcatcggta ccgaacattc cgattgcgaa
     1741 ctggcccagc gcgacgacac aagctcccgt cagcgtggcg atgtcgatga gtgccgccgg
     1801 cttgaaccgg gttgcgtagg cgaggccgtc ggataggatc aggcggccct cggcgtctgt
     1861 gttctgcacc tccacggttt tcccagaaag ggtcttgact acgtcaccag gtcgcattgc
     1921 ccgtcccccg ggcatatttt ccgccgccgg taggatgctg atgagattaa gaggtaactt
     1981 cagacgagcc gccgccctca ttgcggccaa tacctcagcc ccaccggtca tgtcggcttt
     2041 catctgttcc atattctccg ccggtttgag cgagatccct ccggtatcga aggtgatcgt
     2101 cttaccgacg agcacgaccg ggcggtcgtc ttttttcttg gctccccggt actggagaat
     2161 aatgaacttc ggcggttcgt ggctgccttt cgcgaccccc agcaaggcac ccatacccag
     2221 tttttccatc tccttttgtt caaggatttt cagattcaca ccggctgcct tcgcgacggt
     2281 ctttgcctct tgagcaatcc tggtcggtgt catgacgttg gacggatggt tgcagaggtc
     2341 ccgaacgaag gccgtcgctt cagcagttgc gacaccgcga cgaatccctt cggacaactg
     2401 gtgcagctga tctttccgcg aagccagaac ggtcatcgcc atcacgtcct tgctcgaggc
     2461 atcgctgcga tatgtcgtga actgatagct ccctaagata gccccttcgg ccattgtctg
     2521 gccaacgtca atcggcgagg ccccatgtgg cacgacactc ggtaacgcaa ccgtaaaggc
     2581 tccgcatttg gcatggcgaa cccgtttggc ggcatgcccc atcgcctgtc ggatgtgatc
     2641 caaacccagc tcatgtttct tcccaaggcc gacaagaatc
//
LOCUS       DATMTI010000822         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_838227, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000822 DATMTI010000000
VERSION     DATMTI010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838227"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VN039_12990"
     CDS             <1..831
                     /locus_tag="VN039_12990"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWV46913.1"
                     /translation="TLEGASFENPRWWRVDLEDYAATKTLFESVKPHAVFHLSGQATG
                     DPDIRHVLPTFRSQLLTTVHVLTSSAEIGCRRIILTGSLTEPQAGQVDNPPSSPYAAS
                     KWASNAFGRMFQCLYQTPVVIVRPFMVYGPNQNPKKVIPYSIQSLMRGNQPKLTSGEW
                     HADWVYVDDVIDGFIAAMSAPGVEGSTIDLGTGQLVSVEAMIRQIVEIMGVSIEPQFG
                     ALSSRLCEEVRAANIEQTRRMLGWQPKISVYEGIKRTVEWHRSSGPISAVMPSAVKRD
                     "
     gene            874..1716
                     /locus_tag="VN039_12995"
     CDS             874..1716
                     /locus_tag="VN039_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV46914.1"
                     /translation="MSHLMQSNTQVIRIEPSKGRVALQLQELWAYRELLYFLIWRDVM
                     VRYKQTALGAAWAIIQPIFTMLVFSLFFGRLGKIPSDGIPYPIFTYAALVPWTFFSQG
                     LSQASNSLVGSANLIKKVYFPRLCVPIAAVTSGLIDFALAFMVLLGMMLYYGILPGGK
                     VIWLPVLLLLTLTTSLGVSLWLSALNVHFRDVRHIIPFLTQLWLFATPIAYPSSLLSE
                     PWRTLYSINPMVGVVEGFRWALLGAETAPGPMVVVSFLAALAILVSGIFYFRRLEKTF
                     ADIV"
     gene            1759..>2178
                     /locus_tag="VN039_13000"
     CDS             1759..>2178
                     /locus_tag="VN039_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV46915.1"
                     /translation="MATPGLQRGIMGDIAIRVEEIGKKYRIGKPEKYKTLRDTLASSF
                     TAPFLRMGRLLRGEVVDAGQDEQFWALKEVSFEVRQGEVIGVIGGNGAGKSTLLKILS
                     RITDPTTGFAEIHGRVASLLEVGTGFHQELTGRENTYL"
BASE COUNT          483 a          529 c          615 g          551 t
ORIGIN      
        1 accctcgaag gcgcttcatt tgagaaccca cgatggtggc gagtggatct cgaagactat
       61 gctgccacca agaccttgtt cgaaagcgtg aagcctcacg cggtgttcca tttgtccggt
      121 caggcgacgg gtgatccgga catccgtcat gttttgccga cgttccgcag tcagcttctc
      181 acaacggtcc acgtgttgac gagttcggcg gaaatcggtt gtcgccgcat tatcctgacc
      241 gggtcattaa ccgaacctca agcaggtcag gtagacaacc ctccgagttc cccatatgcg
      301 gcatcaaagt gggcaagcaa cgcgtttggc cggatgtttc aatgcctcta tcagacaccc
      361 gtggttattg ttcgtccgtt catggtgtac ggaccaaatc agaatcccaa aaaggtgatc
      421 ccatactcga tacaatcact tatgcgggga aatcagccga aactgacgag tggggagtgg
      481 catgccgatt gggtctatgt ggatgatgtg atagatggct tcatcgcagc catgtcggcc
      541 cctggagttg aaggctcgac gattgatctg gggacagggc agttggtttc ggtagaagcc
      601 atgatccggc agattgtcga aattatggga gtatcgatcg agccgcagtt tggggcgcta
      661 tcgagtcggc tttgcgagga ggtgcgagcc gccaacatag agcaaacacg tcgaatgtta
      721 ggttggcaac cgaagatatc agtctatgaa ggcattaaaa gaacagtcga atggcaccga
      781 tcgtcaggtc cgataagcgc tgtgatgccg tctgccgtta agcgggactg acatgacacg
      841 tttcctctcc actgtcacca tgaaaaaaac tgcatgagcc atctgatgca atcaaatact
      901 caagtcattc ggattgagcc gtccaaggga cgggtggcgc ttcaattgca agagttatgg
      961 gcgtatcggg agctgctcta cttcttgatc tggcgtgatg tgatggtgcg ctataaacag
     1021 acggccttgg gtgcagcatg ggcgattatc caacccatct ttacgatgct cgtctttagc
     1081 ctctttttcg gtcgattggg aaagatccct tccgatggaa ttccgtatcc aatcttcacg
     1141 tatgcagcct tggttccatg gaccttcttt tcccaagggt tgagtcaagc gtccaacagc
     1201 ctggtcggca gtgcgaatct gattaaaaag gtctatttcc cacgactgtg cgtcccgatc
     1261 gcggcagtga cctctgggct catcgatttt gcgcttgcct ttatggtgtt gctcggaatg
     1321 atgctctatt atggtattct ccctggggga aaagtgatct ggctccctgt gttgcttctg
     1381 ctgaccctta ctacgtcact tggtgtctcg ctctggcttt ctgcattgaa cgtccatttc
     1441 cgcgacgtgc gccatatcat acccttcttg acacaactct ggctctttgc aaccccgatc
     1501 gcgtatccga gcagcctatt gtcagaacca tggcgaactc tctatagcat caatccaatg
     1561 gtcggagtag tcgaggggtt ccgttgggct ttgcttgggg cagagaccgc cccaggaccg
     1621 atggtggttg tttctttttt ggctgccctg gcgattttgg taagcggcat attttacttc
     1681 cgtcgcctgg aaaagacttt tgctgatata gtttgatgtc gtggcagccg gcgttcgcag
     1741 cagtaagacg cagacgacgt ggctactccg ggcctgcaga gaggaatcat gggtgatatc
     1801 gccatacgag tagaggaaat cggcaagaag taccgtattg gtaaacccga aaaatacaaa
     1861 acgctgcggg atacgttggc ttcatcgttc acagctccat ttcttaggat gggacgattg
     1921 ttacgaggag aggtggtcga cgctggacag gatgagcagt tctgggcgct gaaggaggtc
     1981 tctttcgagg tgaggcaggg tgaggtcatc ggggttatcg gtggcaacgg tgccggcaag
     2041 agcaccctct taaagatact ctcacggatc accgatccga cgacgggctt tgctgaaatt
     2101 cacggacgtg tcgcttcact cttggaggtg gggacgggat ttcaccagga attaacggga
     2161 agagagaata cttatttg
//
LOCUS       DATMTI010000823         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_115095, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000823 DATMTI010000000
VERSION     DATMTI010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115095"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1371)
                     /locus_tag="VN039_13005"
     CDS             complement(124..1371)
                     /locus_tag="VN039_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46916.1"
                     /translation="MHALLLLGIVNAGCATASLADGEKPPVPITNTPAASETVADASA
                     PTPSQTDQAAPRQSSESVGNVPAPTQPQTDQIAPNRPDAGEVAPASPPNDTPAAVQPQ
                     DSEIPSVQSPDNPPAAQPQADNPPTVEQEPDRTAPTLAEVNPSIMLTGRVWRAKPGIV
                     FLKTPIGLMSLSSKTTLKTLPASQEVSFLIHENHVVVDIVKRTDGTLVHRYLTGPFKR
                     DHEDDTQLIGWTPEGEKAFHMGTFERALASRRDGQSVTVEVDGADTVIGVHDLQFDLQ
                     ISQVAVNGSKAHVLLTGTISKLKSNFIFFRTPIGIVNVNTKIGIKNAKVGQTLTVRMH
                     DHHVVADLSASIDSVPIRRFVTGPLEFADPDRASVRLWTPEGEQIYPADLGKSSLNGA
                     REGTPITVELDGQGDVVEIHRMK"
BASE COUNT          320 a          398 c          475 g          343 t
ORIGIN      
        1 gcggatcggg gatcatccaa ggacgctgcc ggcgagcagt tgatgacgga tgaagcgctc
       61 atacgtagcg cagtctacga ggggattgaa aagagagtca agacgaggtg aaaagcccta
      121 gaattacttc atccgatgga tttcgacgac gtcgccttgt ccgtcgagct ccaccgtaat
      181 aggggttcct tccctcgccc cgttcagcga agattttccc aaatctgccg ggtagatctg
      241 ttccccctcc ggggtccaga gtctgacgct ggctcgatcc ggatcggcaa attccagtgg
      301 acccgtgacg aaacgccgga tcggaacaga atcgatggaa gcggagagat ccgccacgac
      361 gtggtggtca tgcatacgca cggtcaaggt ctgaccgacc ttggcatttt tgatgccgat
      421 cttggtgttg acgttgacga taccgatggg ggtccgaaag aaaatgaaat tggacttcag
      481 ctttgagatc gttccggtta gcaacacatg cgccttggac ccattgacgg ccacctggct
      541 gatctgaagg tcaaactgga ggtcgtgcac gccgataacg gtgtccgcgc cgtccacttc
      601 gaccgtcacg gactggccgt ccctgcggga agcgagcgct cgctcaaatg tgcccatgtg
      661 gaacgccttc tcaccttccg gcgtccagcc gatcaactga gtgtcgtctt cgtggtctcg
      721 cttgaacggt ccggtcagat agcggtggac gagcgtccca tcggttcgct tcacgatatc
      781 aacgacgaca tgattttcgt ggatcaaaaa ggagacttct tgcgaggcgg gaagggtttt
      841 gagcgtggtc tttgaactga gcgacataag cccgatcggt gttttcagga agacgatccc
      901 cggcttggcc cgccagaccc ggcccgtcag catgatggag gggttcacct cagcgagagt
      961 gggtgctgtc cgatcgggtt cctgttcaac agtcggcgga ttgtcggcct gtggctgagc
     1021 ggctggagga ttgtcggggg actgaacaga cggaatctcg ctgtcctgag gttgaacagc
     1081 tgctggagtg tcgttcgggg ggctggcagg tgcaacctcc ccggcatcgg gtcgattggg
     1141 tgctatctga tcagtctggg gctgcgtcgg tgcagggaca ttgcccacag attctgagga
     1201 ctgcctgggt gctgcttgat cagtctggga tggcgtcggt gcagaggcat cggccacagt
     1261 ttccgaagca gccggagtgt tcgtgatcgg tactgggggc ttctctccgt ctgcgagact
     1321 cgccgtggcg cagcccgcat tgactatgcc gaggagaagc aacgcgtgca ctaaccgcct
     1381 agtataggaa aaccgatcca taaagacgcg tgtctgttcc gtatggaaat ggaagtagtg
     1441 aatcttttat tctggttaaa agaaaaccct ttcgagtttc gaagtcgatg gtgacaagat
     1501 ccatctcacg tcactgaccg tctatgcgaa taacat
//
LOCUS       DATMTI010000824         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_582075, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000824 DATMTI010000000
VERSION     DATMTI010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582075"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..592)
                     /locus_tag="VN039_13010"
     CDS             complement(38..592)
                     /locus_tag="VN039_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46917.1"
                     /translation="MDWLLWIALLVVLVISGAGVFALYRFVGKIKCEFQGHLPMLRIT
                     NMSAMNAGEVITLTPEVENVGRGAAHDCIVQLAGWEGRFAVQRIHPKGPRYQRHSVPI
                     VLGQDAPIRMKLLSRCYLRLAYRDCWEQRYECWYPVAQIKNVNTSLYDIQIELSHPEL
                     TEPHPSIREMWKLLRRIHANEASP"
     gene            complement(768..1169)
                     /locus_tag="VN039_13015"
     CDS             complement(768..1169)
                     /locus_tag="VN039_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46918.1"
                     /translation="MKMWPWHTILIVAIIGTALSTADSAETKLLFRSQGATVPAGKSI
                     DLGKVEVASYSSIRVVASTRPDSAANATIQLILMEGDEQYLASLDSIVVPPTSTTTRV
                     YQVPGRKLLIQAAIDGSSGSTSVDVIVYGAQ"
     gene            complement(1431..>2066)
                     /locus_tag="VN039_13020"
     CDS             complement(1431..>2066)
                     /locus_tag="VN039_13020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV46919.1"
                     /translation="LEGKKVLDVACGEGFYARKLRQCGAGEVVGIDRSEAMIRLALDQ
                     ESRHPLGITYRVEDARTESARQEFDLAVSAWLLVYAHNQAELLAMCRGLASRLKSGGR
                     FVTFTGNPNLSAFTHADYRKYGFSIALTDRAYEGAPIVWTVYLDDSSFEIENYYLPME
                     AYETAFIEAGFPDFKVHLPEVSPHPQGVDDGTFWSDYLAHPPAVLIECVKK"
BASE COUNT          501 a          536 c          545 g          484 t
ORIGIN      
        1 taaaatggta cggttcagga acccagaatg caaggggtca aggtgaggcc tcattcgcat
       61 gaattctgcg aagcagcttc cacatttctc tgatcgatgg atggggttct gtcagttccg
      121 ggtgagagag ttcgatctga atgtcataga gagaggtgtt cacgttcttg atttgggcga
      181 ccgggtacca acattcatac cgttgttccc agcagtcgcg atacgctaac cgcagatagc
      241 atctgctcaa caatttcatg cggatcggtg catcttggcc caggacgatg ggcactgagt
      301 ggcgttgata gcgagggcct ttgggatgaa tgcgttggac cgcaaaacgc ccctcccatc
      361 ctgccaactg gacgatacaa tcatgggctg ctccacggcc cacattttcc acctcgggag
      421 tcagcgtgat cacctctcct gcgttcatcg ccgacatgtt ggtgatgcga agcatgggga
      481 gatgcccttg aaactcgcac ttgatcttac cgacgaatct atacagcgcg aagacccctg
      541 cgccactgat gacgagtaca acaagcaagg cgatccacaa tagccagtcc atgataaagt
      601 tgtcctacgt ggctcttgat ccgaaacggt gaaaccctac gtgcaggaga ggcgagtgtc
      661 aactaaccgt gtgtgcacga gacgagatca catggacaag ccgcgtttcg ctcgaattca
      721 gataaggtta agacaggaaa actgcgcgac agttcgtcgc agtatcatca ttgggccccg
      781 tacactatga cgtcgacact tgtagagcct gagcttccat ctatagccgc ttgtatgagc
      841 aattttcttc ctggaacttg atagaccctt gttgttgttg atgtcggtgg gacaacgata
      901 gagtctagag aagctaggta ttgctcatca ccctccatga ggataagttg aatcgtcgca
      961 tttgcagcgg agtcgggtcg tgtgctcgcg accactctaa ttgagctata ggaggcaacc
     1021 tccaccttgc ccaaatctat agattttcca gcagggacgg tagctccttg agatctgaac
     1081 aacagttttg tctcagcgga gtccgctgtc gataatgccg taccgatgat ggcgactata
     1141 agaatcgtgt gccaaggcca catcttcatg acccgacctc catcctaagg tgtaagaacg
     1201 cgtcccgcca tttaaggggc ataataacgt agccgatcac ggttacttgc tacaatacct
     1261 tgattgcgtt tttgctcagt ttgatcgtgt tgcggtggct gaggaagtgg aaaactgatc
     1321 cgtttgtccg gcaagtgacg cgcataacga ttctgtctaa tgcggccgag gaaacgagcc
     1381 ggtgctttgc cacaggcgtc ggtcgttaaa agttcacaac gttcctctgg ttattttttc
     1441 acacattcga tcagcactgc gggagggtga gcgaggtaat ccgaccagaa tgtcccatca
     1501 tcaacaccct gcggatgtgg agagacctcc ggcaaatgaa ccttgaaatc ggggaatcct
     1561 gcctcaataa acgcggtctc gtacgcctcc atgggtaaat agtaattctc gatctcgaat
     1621 gaagagtcgt ccagatagac cgtccagacg attggagctc cttcgtaggc gcggtctgtg
     1681 agtgcaatac tgaaaccata cttccggtaa tcggcatgcg tgaaggcgga gagattggga
     1741 tttcctgtga aggtgacgaa ccgtccaccc gacttcaaac ggctcgcgag cccacgacac
     1801 atggccaaga gctcggcctg gttatgcgca tagaccaaga gccaggccga caccgctaga
     1861 tcgaactcct gccgagcgct ttctgtacga gcatcttcga cgcggtatgt aatgcccaac
     1921 ggatgacgtg attcctgatc gagggctaaa cggatcatgg cctcggagcg atcaatgcct
     1981 acgacttccc ccgcaccgca ttgccggagt ttgcgtgcat agaaaccttc accacaggcg
     2041 acatcaagga cttttttccc ttcaag
//
LOCUS       DATMTI010000825         1727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_284948, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000825 DATMTI010000000
VERSION     DATMTI010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284948"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /gene="dnaB"
                     /locus_tag="VN039_13025"
     CDS             <1..1119
                     /gene="dnaB"
                     /locus_tag="VN039_13025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWV46920.1"
                     /translation="LKSRGELEAIGGAAYLAELVQIVPSSANIRYHCKIVRDKAVARQ
                     LISTSTEVLTRGYEGTASIDDLLDFAERSVFGIAQGKLERSFSPISQVIKESLDVIDK
                     LSKRKEHVTGVPTGYYDLDDITAGLQASDLVVVAGRPSMGKTSLALGFATHAAIHANT
                     VVGVFSLEMSKPQIVLRMLSSEARVDSHALRTGKLQKEDWWRLAEAAGRLEQAPIYID
                     DTGGITVQQMRGKARRLKAEKGLDLLIVDYLQLMQGRSDSESRQQEISDISRSLKALA
                     KELNVPVVALSQLSRAVEARKPPIPMLADLRESGAIEQDADVVIFIYREDVYDSNSER
                     KGIADIIVSKHRNGPIGKKELFFQDRFAKFESLDLREA"
     gene            1204..>1727
                     /locus_tag="VN039_13030"
     CDS             1204..>1727
                     /locus_tag="VN039_13030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV46921.1"
                     /translation="MRNTRMVLYDRSLRQKGMLLVRTLLISLFLSGAIVACAASPQTK
                     TTLSPPATAVQPPPSAPESDATYHFMMGHQAELAQDLDAALKEYHTALKVDPKSREVK
                     SRLAALYFAMGDVTQAVQYADEVGEGTGQEPQMLTHMAGILASAGKQERALELLDKAI
                     ESDPERGESYFPKGL"
BASE COUNT          397 a          468 c          501 g          361 t
ORIGIN      
        1 ttgaaatctc gcggtgaact tgaggccatc ggaggtgcgg cctatctcgc cgaactcgtc
       61 cagatcgttc ccagttccgc caatattcgg tatcactgca agatcgttcg ggacaaggcg
      121 gtggcccgtc agttgatcag cacctccacc gaggtgctga ctaggggcta tgaaggcacc
      181 gcctcaatcg acgacctgct cgattttgcc gaacggtcgg tcttcggcat tgcgcaaggc
      241 aaactggaac ggtcgttcag cccgatcagt caggtcatca aggaaagtct ggatgtcatc
      301 gataaacttt cgaagcgaaa agaacatgtc acaggagtgc ccaccgggta ctatgacctt
      361 gacgatatta ccgcagggct tcaggcctcg gatttggtgg tggtggccgg gcggccgagc
      421 atgggtaaga cgagcctcgc cttggggttt gcgacgcatg ccgccattca tgccaatacg
      481 gtagtgggag tcttcagctt ggaaatgtcc aagccgcaga tcgtccttcg tatgctcagc
      541 tctgaggcgc gagtcgattc ccatgcgctg cggacgggga agcttcaaaa ggaggattgg
      601 tggcgcttgg cggaggcggc cggccgattg gaacaagcac cgatctatat cgatgacacg
      661 ggcggcataa ccgtccaaca gatgcgaggc aaagcccgcc ggctcaaagc agagaaagga
      721 cttgatcttc ttatcgtaga ttacctccag ctcatgcaag ggcgaagcga ttcggagtcc
      781 cgtcagcaag aaatctccga tatttcccgc tcgttaaagg cattggcgaa agagctgaat
      841 gtgccggttg tagcgctatc tcagttgagt cgtgcggtgg aggcccgtaa gccgccgatc
      901 cccatgctgg ccgatttgcg ggaaagcgga gcgatcgaac aggatgccga tgtggtcatc
      961 ttcatttacc gagaagacgt gtacgactcg aattcggaac gcaaaggaat tgccgacatc
     1021 atcgtcagta agcatcgcaa tggtcccatt gggaaaaagg agctgttttt ccaggaccgc
     1081 ttcgccaaat tcgaaagcct cgatcttcgc gaagcatagc cgggggagtc gtttgcgcat
     1141 cgccccgaca ctcggtataa tcctttcatt ggcccgccac cacttgtcct gacgaacggt
     1201 attatgagga acacacgaat ggtcttgtac gatcggtcgc ttagacagaa gggtatgctc
     1261 ttagtgagga cgctgctgat ctccctgttc ctctccggcg ccatagtcgc ctgcgcggcc
     1321 tccccccaga ccaagacaac cctttctcca cctgccacag cagtgcagcc tccgccatcg
     1381 gctcccgagt cggatgccac ctaccatttt atgatgggac atcaagcgga actcgctcag
     1441 gacctggatg ccgcattgaa ggaatatcat accgctttga aagtcgaccc gaaatcccgc
     1501 gaggtcaaat ctcggttggc cgctctgtat ttcgcgatgg gagatgtgac tcaggccgtg
     1561 cagtatgcgg atgaagtcgg agaaggaacg gggcaagaac cgcaaatgct gacccatatg
     1621 gccggcatct tagccagtgc ggggaagcag gagcgcgcct tggagttatt ggacaaggcc
     1681 atcgagagcg atcccgaaag aggagaatcc tactttccga agggact
//
LOCUS       DATMTI010000826         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_30688, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000826 DATMTI010000000
VERSION     DATMTI010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30688"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1492
                     /locus_tag="VN039_13035"
     CDS             332..1492
                     /locus_tag="VN039_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HWV46922.1"
                     /translation="MRPMGRGGLSVAQKAELWRRWKYGQSLSEIGRALGKHAGSIYGV
                     LSSKGGVMPAVRRRSQWALTLAEREEISRGLASDTSIRQIASTLGRAPSTISREIHRS
                     GGTHRYRAAEADTRAWARTRRPKPCRLATSPILRRIVASKLRVEWSPEQIAGWLKRAF
                     PYERTMQVSHETIYRSLFIQARGVLKKELLRALRSRRRMRQAKWASTAGQPRGQIIDG
                     LSIGDRPAEVADRAIPGHWEGDLLTGSHNTHVATLVERRSRFTMLVKVPGKDTASVVT
                     ALSQQIGTLPVALRRSLTWDRGMELAQHKRFTLATKVQVYFCDPQSPWQRGTNENTNR
                     LLRQYLPHGTDLSRYSQAELNRIALRLNQRPRKTLGFQTPAAILEAGVALTS"
     gene            1493..>1822
                     /locus_tag="VN039_13040"
     CDS             1493..>1822
                     /locus_tag="VN039_13040"
                     /inference="COORDINATES: protein motif:HMM:NF024565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV46923.1"
                     /translation="MYQYDDQLWGGFEGYRNSVEKFTSKVLREHASNTATLLTLEELE
                     TKAQSIFGPTPTAETPAPAVDISKLLEHETNLILKKRVIGKDDVDITAMIKKLSNSDW
                     VREGRSFY"
BASE COUNT          445 a          480 c          541 g          356 t
ORIGIN      
        1 ttcgggtgaa ccctcccatc gttcactggc atgcccttcg tgggaatcgt ccgtaacact
       61 tgtctcactc gcgacgttct gatgacctga gcgtgcttgg gcctgtacct ctggaatgct
      121 agcaaacgaa agaataagaa ccaggaagac agacagccga taagcaccgc gattcatata
      181 cgaagaacca ccgatgagaa gagttagggc aaagtggtcg cgcacgatag catagatcgt
      241 tccatacatc tattcgaaag ataggaaacg gtagattcaa ccgatcacgg caacaccatt
      301 tggtctacga tggatatgac ggaggatcgg tatgagacca atggggcgag ggggcttgtc
      361 tgtggctcaa aaagcggaac tgtggcgacg atggaagtat gggcagtcat tgagcgaaat
      421 cggtcgagcc cttggtaagc acgccggctc gatttatggt gtgttgtcat cgaagggggg
      481 cgtcatgccg gccgttcgcc ggcggtcgca gtgggcattg acgttagcgg agcgagaaga
      541 aatttcccgt ggcttggcga gtgacacatc cattcggcag atcgccagta cgctcgggcg
      601 agcgccgtcg accatcagcc gagaaatcca ccgctctgga ggcacgcaca ggtatcgggc
      661 cgccgaggcc gacacgcgag cctgggctcg cacgcggcgc ccgaaaccgt gtcggctcgc
      721 cacctctcct atactccgga gaatcgtcgc gagtaaattg cgagtggagt ggtcaccgga
      781 acaaatcgca ggatggctca aacgggcatt cccctatgaa cggaccatgc aggtgtcaca
      841 cgaaacgatt tatcgcagct tgtttattca agcccgcggg gtactcaaga aggaattgct
      901 cagggcgctc cgctctcggc ggaggatgcg acaggccaaa tgggcgagca ccgcgggcca
      961 gccgcgtgga cagatcattg acggcctttc tattggggat cgaccagctg aagtcgccga
     1021 tcgagccatt cccggtcact gggaagggga tttgctcacc ggatcgcaca atacgcacgt
     1081 cgcgacgttg gtggaacgcc ggtcgcgctt cacgatgctg gtgaaagtac ccgggaaaga
     1141 taccgccagc gtggtgaccg ccctgagcca acagatcggc acgctaccgg tggccttgcg
     1201 gcgatcgttg acctgggatc gcgggatgga attggcccag cataaacgat tcacccttgc
     1261 gaccaaggtg caggtctatt tttgtgaccc gcaaagtccc tggcagcgag gcaccaacga
     1321 aaatacgaat cgcttgctgc gtcagtacct cccccacgga acagacctct cacgctattc
     1381 ccaggcggag ttgaacagga ttgccttacg gctcaatcaa cggccgcgaa agacattagg
     1441 ctttcagacg ccagcggcta tattggaggc aggtgttgcg ctcaccagtt gaatctacca
     1501 gtacgatgat caattatggg gtggctttga gggatatcgg aacagcgtag agaaatttac
     1561 aagtaaagtg cttcgggagc atgcgtcgaa caccgcaacg cttctcacct tagaggagtt
     1621 ggagacgaag gcccaaagta tattcggacc gacaccgacg gcagaaacgc ccgctcccgc
     1681 tgtcgatata tccaagcttc tcgagcatga aacaaaccta attctgaaga agcgggttat
     1741 cggcaaggat gatgtcgata ttaccgcgat gattaagaag ctcagcaata gcgactgggt
     1801 gcgcgaggga agatcgtttt at
//
LOCUS       DATMTI010000827         1710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_115218, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000827 DATMTI010000000
VERSION     DATMTI010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115218"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..>1710)
                     /locus_tag="VN039_13045"
     CDS             complement(75..>1710)
                     /locus_tag="VN039_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV46924.1"
                     /translation="ALAPPAPKDYRIAAQRMGFGIITEADAEFLAGQSPKRAELGLRR
                     GRTLEVSLGRLRQYPHIEKGKATSDLSSEGPTLHDLSGYGEARAWGVELAEDLAAWKA
                     GDLAWDAVDRGILLSGAPGSGKTSYAAALARTCGVPLILGSAGRWQATGHLGDMLKAM
                     RKAFTEAAMQAPSVLLIDEFDSFTGRDDRTGSSDNYHRQVVNALLELLDGAQSHEGVV
                     VIGATNYPDIIDPALLRAGRLERHFSIPFPDATARHDILRFYLQGDLVDDVLDGVVAA
                     SDGWSGADIERCVRDARRHARRARRSMLLSDLIAAMPPVVQVPMAVQRCVAAHELGHA
                     ILGVLLEVDPLVSVSIERTIQIGSRSQSLGHTAFEERLFSRKTSTYFRNKIATMLGGI
                     AAEQLLFGEYGNGAGGDPSADLNRATDLATMAEVKWGLGKTLSVEPGDSPRELSVFRH
                     RRKGLRKCVEATLQSEFRRAKSLLEEHRNILLDLHAGLLREGSLQADVIRAAMGLTGD
                     RNSVNGSIMAVEAPTSKSKNMCLSAGISGSIPSEGR"
BASE COUNT          367 a          522 c          471 g          350 t
ORIGIN      
        1 ctgcaaccta tgccggtacg gacgattgaa gcgaattttt aaccatattc gaagtcttcc
       61 aattatcctg taattcaacg tccttctgaa gggatcgaac ccgaaattcc ggcggagagg
      121 cacatatttt tagacttcga agttggtgcc tcgacagcca tgatactgcc attcacggag
      181 tttcgatccc cggtaagccc cattgcagcc ctgatcacgt cggcttgcaa actaccttcc
      241 cgcaaaagac cggcgtgaag gtcaagaagg atgttccggt gttcctcaag tagagatttc
      301 gcgcgccgaa actccgactg caatgtggct tcgacgcatt tgcggagacc cttgcggcga
      361 tgacggaata ccgacaattc acgagggctg tcgccaggtt cgacagacag cgtcttaccg
      421 aggccccact taacttctgc catcgttgcc aagtctgtcg cacggttgag gtctgccgac
      481 ggatcaccgc cggctccatt cccgtattca ccgaagagca attgttcggc tgcgatgcca
      541 ccaagcatgg tggcgatctt gtttcggaaa taggtcgatg ttttccgact gaacaggcgc
      601 tcctcaaacg ccgtatgacc cagagactgt gatcggctgc cgatctgaat cgttctctcg
      661 atcgagaccg acaccaacgg atcaacctcg agcaggaccc cgagaatagc atgtccaagt
      721 tcgtgggcag cgacacagcg ctgtaccgcc atcggcacct ggacgacagg cggcatggca
      781 gcgatgagat cactgagtaa catcgagcgc cttgctcttc tcgcatggcg acgggcatcg
      841 cggacgcacc gttcgatgtc agcaccagac cagccgtcgc ttgccgccac cacgccgtcc
      901 agcacgtcgt cgacaaggtc tccctggaga tagaagcgaa ggatgtcgtg gcgcgccgtc
      961 gcatccggaa aaggaatgga gaagtgacgt tcaagccgcc ccgctcgaag aagcgctgga
     1021 tcgatgatgt ctggatagtt tgttgcaccg atgacgacaa cgccttcatg tgactgtgct
     1081 ccgtccaaca attccagaag cgcgttgacc acctggcgat ggtagttgtc gctgctgccg
     1141 gttctgtcat cacggccggt gaaactgtcg aattcatcaa tcagtaagac cgacggcgct
     1201 tgcatcgcgg cctcggtaaa tgccttacgc atggctttca acatgtcgcc gagatggccc
     1261 gtcgcctgcc atcgtcccgc ggaaccgagg atcaacggca cgccgcaagt cctcgccagc
     1321 gccgctgcat aggacgtctt gccggacccg ggtgcgcccg aaagcagaat gccacgatca
     1381 acggcatccc acgcaaggtc accggctttc cacgctgcca ggtcttcggc aagttcgaca
     1441 ccccacgctc tagcctcgcc atatcccgaa aggtcgtgca gtgtcggtcc ctccgaggag
     1501 aggtcggagg tagctttccc tttttcgata tgtggatact gacggagacg cccgaggctg
     1561 acctccaagg ttcgaccgcg tcgcaagccc agttccgccc gcttgggtga ttgacctgcc
     1621 agaaattcgg cgtccgcttc cgtgataatc ccgaacccca ttcgctgcgc ggcgatgcga
     1681 taatccttgg gagccggcgg ggccaaagcg
//
LOCUS       DATMTI010000828         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_838391, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000828 DATMTI010000000
VERSION     DATMTI010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838391"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1112
                     /locus_tag="VN039_13050"
     CDS             90..1112
                     /locus_tag="VN039_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWV46925.1"
                     /translation="MVKLISLLSPLFAGFLGIALTAPTQSAQLEQDRGTWHAAAPLPT
                     KRTEVAAAMLDGKIYVVGGFEKPGLGNLLNFAITPSVEMYDPVTDRWTPKTPMPVGLH
                     HVGIGVVDGRLYVIGGYSRSGLSVWSPVATVYAYDPVTDTWTGRAPMPTARGALAVTE
                     HEGKLYAIGGYDRKANNVQVEVYDPVRNVWTTAASLPTSRDHLGTATVAGKIYAIGGR
                     IDGDYHRNLAVMEQYDPATDRWRRVADLPTARSGIAAAAVEGRIYVFGGEGSSGTFSE
                     NEVYDPASDTWQRMAPMPTARHGIGSAVVRGRIYVISGGPIPGGSFSDLNEVFTIPST
                     SISMAE"
     gene            complement(1812..>3676)
                     /locus_tag="VN039_13055"
     CDS             complement(1812..>3676)
                     /locus_tag="VN039_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWV46926.1"
                     /translation="QVVNGTAGRADADIDAPEAWDISTGSPNVIVAVIDSGIAYDHPD
                     LAPNIWTNPKEIPANGVDDDGNGLVDDVHGWDFRMNDGDPIDPIALNPDGNPGHGTHV
                     AGIIAGAGNNGTGVTGVMWTAKLMALRAGGVDRSVSTTAIVSAIHYAVDNGARVINAS
                     FGGPDCSLALYDTVNAANAAGVLFVAAAGNGGSDNDNVPNFPSNFSAPSVCDGQQKAA
                     LSNVIAVAATDQNDELAIFSSTAASNFGANTVQVAAPGVSINSTKPTSNVTNVLLHNF
                     DSNPTGLGYVFGGTNNTWGFTNSTSFSQPNSLSDSPNGNYQNNTDSFAIGPVFSTEGQ
                     RGCLLDSRVRLQTDGIDGVVVETSRNSGASWQPIRGVTEITNAPFDPFTWVDVADGVA
                     GSRFRFRFISDEGQVFDGVYVDDVRVACVAGPPSGTTDYQFLQGTSMATPHVAGLAGL
                     LLSVDPNLTVSQLRNAILNTVDRKASLRGKVSTGGRINARAALASVVANFAVTVNKAG
                     AGTGTVTSNPSGIDCGTTCNGQFLQGRTVNLTAVPDPGSVFAGWSGDCSGTDPCSLTQ
                     NTTVTATFNIASSSPVPSDNVGGGCTVAPGTTGDMLLPAMLVMSLVVLLWRARR"
BASE COUNT          850 a          998 c         1045 g          783 t
ORIGIN      
        1 cctcaacctg gatgctactg gaccttaagc aggtatagaa tgaaaccatg tccttcttca
       61 cgagatcatc agccagagga actcgctttg tggtgaaact gatttcgtta ttgtcaccgc
      121 tctttgctgg gtttttgggg atcgcgctga cggcaccaac tcaatcagcg caactcgaac
      181 aggatcgagg cacatggcat gcagcagcgc cgctgccgac gaaacggacg gaggtggctg
      241 cggcgatgct ggatgggaag atctacgtcg ttggagggtt tgagaagccc ggcttgggca
      301 atttgctgaa tttcgcgatc acgccgtccg ttgaaatgta tgatccagtg acggaccgat
      361 ggacgcccaa aacgcctatg ccggtcggct tgcaccatgt gggaatcggt gtcgtggatg
      421 gacgattgta cgtcataggc ggatacagtc gatcgggact cagcgtctgg agcccggtcg
      481 cgacggtata tgcctacgat cctgtcacgg acacctggac cggtcgcgct ccgatgccga
      541 ctgcacgtgg tgcattggct gtgaccgaac atgagggaaa gctctatgcc atcggcggct
      601 acgacaggaa ggccaacaat gtgcaggttg aggtgtacga tcccgtgcgc aacgtctgga
      661 caacagcggc atctctacca acctcgcgcg atcatctcgg caccgcgacg gtcgccggga
      721 agatttatgc catcggcgga cgcatcgacg gtgactacca tcggaatctt gccgtcatgg
      781 aacagtacga tccggcaact gatcgctgga ggcgcgtcgc ggatcttcca accgcgcgga
      841 gcggtatcgc agccgctgcg gtagaaggac ggatctacgt attcggcggc gaagggtcta
      901 gcggcacatt cagtgagaac gaggtttatg acccggccag cgatacctgg cagcgtatgg
      961 ctccgatgcc gactgctcga catggtatcg ggtcggcagt agtacggggg cgcatttatg
     1021 tcatcagtgg cggaccgatt cccggaggct ccttcagcga tctgaacgaa gtctttacca
     1081 taccgtcgac aagcatctcg atggctgagt gatttttatg tcttagcctt ccaagccttt
     1141 tcaagcaaat ggcccctgac aatcatgaag gttcctccac gatggcctgc aagcgcggtt
     1201 ctgttgagca tcctaaaagg agtttggaag agtcaggcca ccgaggagaa gaaagtaacc
     1261 ggcattatag aaggcatgca gggtgatgga gggatgcaca gatccaaagc gatctcgtac
     1321 gaagccgaaa cagagagaag gaacgaacac gagcaaggac cacgaaacgg aatggctcgc
     1381 cagatgggcc aatgagaaca ggagcgatgt caggaggttg gagacggata gaccaatcca
     1441 tgtcttctgc ctcgccgtgg attgcaacaa acgaccctgc acaatgccac ggaagagcaa
     1501 ttcttcgaat agaggctgcc atagggcgac ggagagaaaa gccggtgacc atattgtatg
     1561 ccgaggcagt ggttgaaatg gaaaaacctc gatcatggaa atccatacta tcgggccaag
     1621 ggctaacaat aggagaaatg cagaatcacg atggaaggga cgattgtatt gaaggcccag
     1681 taacaccatg acttgcgctg agcggcttag tagatggagt acacgaaagg aaacgccctg
     1741 ggccacgacc atgcggtcga ggtcgagctt tcgctgtgac cgctctcctc gtgctctgaa
     1801 actcagcctc atcagcgtct tgccctccac aacaagacaa ccagagacat gacgagcatg
     1861 gcgggtagca acatgtctcc agttgtacca ggggcgacag tacagcctcc tccgacgtta
     1921 tcgctgggaa ccggcgaaga tgacgcgatg ttgaaggttg cggttaccgt cgtattttga
     1981 gtgagcgagc agggatcagt tccactacaa tctccactcc accctgcgaa gacagatcca
     2041 ggatcgggta ctgcggtgag gtttacggtg cgaccttgga gaaactgtcc attgcatgtc
     2101 gttccgcaat caattcctga tggattggat gtcactgtgc cggttccggc gcctgccttg
     2161 ttgaccgtga ccgcgaagtt tgccacaacg ctggccaatg cggctcgggc attgatgcgc
     2221 ccaccggtgg acacctttcc tcttaaggaa gccttccgat ccactgtgtt gaggattgcg
     2281 ttcctgagtt gtgatacggt gagattggga tcaaccgaga gcaacaagcc ggcgagtcct
     2341 gccacatgcg gagtggccat cgaagtaccc tgaagaaact gatagtccgt cgttcccgat
     2401 ggcggacccg ccacgcaggc gacgcggaca tcgtctacat acacaccgtc gaagacctgg
     2461 ccctcatccg atatgaatcg gaagcggaac cgtgaaccag ccacaccatc cgcgacatcc
     2521 acccaggtga aaggatcaaa tggggcattg gtaatttctg tgactccacg aatgggctgc
     2581 caactggctc cgctgtttct tgacgtttcg acaacaacac cgtctatacc gtcggtttgc
     2641 aggcgaacgc ggctatcaag cagacacccc cgctgtccct cggtactgaa cactggtccg
     2701 atcgcgaatg aatcggtgtt gttctgatag tttccgttcg gactgtcgct cagactattc
     2761 ggttggctga atgatgtgga attggtgaac ccccaggtat tattggttcc cccaaagaca
     2821 tacccgagcc cggttgggtt cgaatcgaag ttatgcagga gcacattcgt aacgttcgac
     2881 gtgggtttcg tgctgttgat gctgactccc ggcgcggcca cctgaaccgt gttagcccca
     2941 aaattggacg ccgcggtgga actgaaaata gccaattcgt cattctggtc cgtggcggcc
     3001 acggcgatca cgttcgagag agcagccttc tgttgcccgt cacataccga aggagcgctg
     3061 aaatttgagg ggaagttcgg tacgttgtcg ttatccgatc caccatttcc tgctgctgca
     3121 acaaacagta cgcctgccgc attcgcggca ttcaccgtat catagagagc caggctacag
     3181 tcaggtccgc caaaactcgc gttgatgaca cgtgcgccat tgtcgacggc atagtggatc
     3241 gcactgacaa tagcggtggt ggatacggac cgatcgaccc ctccggctct tagggccatc
     3301 agcttcgcgg tccacatgac ccctgttacc ccggttccat tgtttccggc tccagcgatg
     3361 attcccgcga catgagtgcc atgtcccggg ttaccatcag gattgagagc gatcgggtca
     3421 atcgggtcac cgtcgttcat gcggaaatcc catccatgca catcgtctac taatccattt
     3481 ccatcgtcat caacgccatt cgcaggaatc tctttcggat tcgtccagat attgggcgcg
     3541 agatccggat gatcataggc aatacccgag tcaatgacgg cgacaatcac gtttggactt
     3601 cccgtgctga tatcccaggc ttccggcgca tcgatgtcgg catccgccct tcctgctgtt
     3661 ccattgacaa cctgac
//
LOCUS       DATMTI010000829         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_72774, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000829 DATMTI010000000
VERSION     DATMTI010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72774"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1039
                     /locus_tag="VN039_13060"
     CDS             176..1039
                     /locus_tag="VN039_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWV46927.1"
                     /translation="MKILLTVDGSDHSYEAVRSLKYLSRAEELHIVHVIDVPSPAYPM
                     MMPEVAQELYETVERNMRDDGTRLLDRIVSLLPLDVGPVTKHLVVGSPVDQIVALAEQ
                     LKVGLILLGTRGLGPIKERLIGSVSHRVLTFGPGAKLILPGPLKTLHDILLPLQGTYD
                     ADHALAFLQQKPFRDSPTITLFTVLPHTRPPWPVDAVSVEQMETHALGKAKDFLEEIA
                     VKLKSYGHQTCIATMLGTPVDGILQEAKVLNPDLILMGSRGRRGLTRMVLGSVSHALL
                     HQGTYPLMIFG"
     gene            1060..1224
                     /locus_tag="VN039_13065"
     CDS             1060..1224
                     /locus_tag="VN039_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWV46928.1"
                     /translation="MPMYDDKCLDCGKESLIVVTLKEHEAGMATCPACSSKKLQQLFS
                     PFIAHTTKKS"
     gene            1685..2032
                     /locus_tag="VN039_13070"
     CDS             1685..2032
                     /locus_tag="VN039_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWV46929.1"
                     /translation="MGLWTFFVLISLVLPPLYAQDYLPDRARGKAVYERHCQSCHGPS
                     GRGDGPAAASLKVPPTNFQRFQSFLKSDEELLRTIEHGVVFSPMHSWRGRLTDGEMQD
                     VVVYIRLLSQQAQ"
     gene            2040..2669
                     /locus_tag="VN039_13075"
     CDS             2040..2669
                     /locus_tag="VN039_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWV46930.1"
                     /translation="MSSVNRSSDCRAPSNPLIALAPHDALLLVDIQNDFLPGGSLETS
                     DGDAIIPILLDYVRLFQTHSLPIFLTRDWHPPDHCSFKPRGGPWPVHCVAGSPGSLPP
                     PSFPTPISAVIIYKAIDPDQEAYSAFHHTALDRHLRALTVHRLFIGGLATDYCVLNSV
                     KDVRMRGYDVCLLMDGMKAVNLQSDDGRHAEEEMVRLGAAPVRWEMLKI"
BASE COUNT          554 a          788 c          763 g          642 t
ORIGIN      
        1 ttcacatctc acgttttatc tcacttcccg gggcggcatt ggacttgctc agcatcctca
       61 gtagggaaag gtgaggtcgt atgacgtgcg cctgccatgg ttgatggtcg acagcttcat
      121 tgcctgaatg actcatgtga acgaagcagc agatccacag tctgaaggtg acgctatgaa
      181 aatcttgctc actgtcgacg gttctgatca ttcatatgaa gcggttcggt cccttaagta
      241 cctgtctcgt gccgaggaac tgcatatcgt gcacgtgatt gatgtcccta gcccggccta
      301 tcccatgatg atgccggaag tggcgcaaga actgtacgag acggtcgagc gaaacatgcg
      361 cgacgatggg actcgcttgt tggatcgcat tgtgtcgttg cttccattgg atgtcgggcc
      421 ggtcacaaag catctggtcg tcggatctcc ggtggaccag atcgtggcct tggctgaaca
      481 actcaaggtc gggctgattc tcttgggcac cagagggctt ggaccgatca aggaacggct
      541 catcggaagc gtctcccacc gagtgctgac cttcgggcct ggtgcgaaac tgatccttcc
      601 cggtcccttg aaaacgcttc atgacatatt gcttccctta caaggaacct atgatgcaga
      661 ccatgccctc gccttccttc aacagaaacc ctttcgtgat tcccccacga ttactctgtt
      721 caccgttctt ccccatacca gacctccctg gccggtggat gcggtgtcgg tcgagcagat
      781 ggaaacccat gccctcggca aggcgaagga cttcctcgag gaaatagccg tcaagctcaa
      841 atcctatggc catcaaacct gcatcgcgac catgttgggt actccggttg atggaattct
      901 tcaggaagcc aaggtcctga atccagacct tattctcatg ggatcccggg ggcgtcgcgg
      961 cctcacccgc atggtactcg gcagtgtttc acacgctctg ttgcaccagg gaacttatcc
     1021 cctcatgatt tttggctgat tgtcgaaaga gggtgcgcca tgccgatgta cgacgataag
     1081 tgccttgatt gcgggaagga atcgttgatt gtggtgacct tgaaggagca tgaagccggg
     1141 atggcgacat gccccgcgtg cagcagcaaa aaactccagc aactcttcag cccgtttatc
     1201 gctcacacca caaagaagag ttagcagcgt gttgaaaaaa cccgccagcg gcgttctcgc
     1261 atatggcacg ggaaaacacc tgttccgacg ggcgatggag cgggcgggta agagtaggtt
     1321 acgcttcgct cttaagacac tgggcgctca cgggacacgg cccgctcacc atctcgcggg
     1381 cgtccacagg cgtgccatgt cttattcggc atggcgtgga cctcgcgccc gtccgcagac
     1441 atggagaaat tctgacactg ccggctcacc gactcgccgg cgcgcacaaa cgtggcgctc
     1501 tttcttcatc gcgccgtgcg cctcattatt cttcgcgtcg cggacctcgc tgcggccttg
     1561 cctgcggaac ggcgcgtctt ggcgcgccgg gggggtgggc gagtgagaat gacggctttt
     1621 ttgaacactc tgcagaacta ggttgatcat gcaatcgcca tgcatagaga accgattctt
     1681 catcgtggga ctgtggactt ttttcgtcct gataagcctg gtgctccccc ctctatacgc
     1741 ccaggactat cttcctgacc gcgcacgcgg aaaagccgtg tacgagcggc attgtcaaag
     1801 ctgccacgga ccaagcggcc gaggcgatgg acctgccgcc gcttcgttga aagtcccgcc
     1861 gaccaacttt cagcgatttc aatcgttcct gaaatccgat gaggagttac tgcggaccat
     1921 cgagcacggc gttgtcttca gtccgatgca ttcgtggcgt ggccggctca ccgacggcga
     1981 aatgcaagac gtcgtggtat atattcgtct gttgtcgcaa caggcacaat gacagagtca
     2041 tgtcctcggt caacagatcg tcagactgcc gggctcccag caaccccctg attgcgctcg
     2101 caccgcatga tgcgttgctt ctcgtcgaca ttcagaatga ttttctaccg ggagggagcc
     2161 tggaaaccag cgacggtgat gcgatcattc cgattctatt ggactatgtt cgcctctttc
     2221 agactcacag tctgccgatt tttctcactc gtgattggca cccgcccgac cattgctcgt
     2281 tcaaaccccg gggaggtccg tggcctgtgc attgtgtcgc cgggtctccc gggtctctgc
     2341 cgccgccgtc gtttccgact cccatctccg ctgtcatcat ttacaaagcc atcgacccag
     2401 accaagaagc ctattctgct ttccaccaca ccgctttgga tcgtcacctg cgggccttga
     2461 ctgtgcatcg tctcttcatc gggggcttgg cgactgatta ttgtgtgctg aattcggtca
     2521 aggacgttcg gatgcgaggc tatgatgtct gtttgttaat ggacggcatg aaggcggtca
     2581 atctgcaatc cgacgatggt cgtcatgcgg aagaggagat ggttcgcctg ggagccgcgc
     2641 ctgttcgctg ggagatgctg aagatatgaa tccttcggcc agcgccctgc tgacggatct
     2701 gtatgagctc acgatggccc aggcctactt agaacaaggg atggacg
//
LOCUS       DATMTI010000830         1744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_709066, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000830 DATMTI010000000
VERSION     DATMTI010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709066"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VN039_13080"
     CDS             complement(<1..617)
                     /locus_tag="VN039_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46931.1"
                     /translation="MIRCTVVLGAAFLLLTLTSTSSLAQSPETVLQTAIDSLSAERML
                     ADIRTLSGPSFNGRQSGTKADLESARWVAQEFLSAGLRLPLVRNGSLIIPSLTGKDGA
                     SLGMMATLIPTPLIAPDPVLRVGPTDHLAAKTLGSDYFPVFDSPSADLQAQIVFVGYG
                     IVDPAQGIDDYAGVDVNNCIVLFLRGKAEHSPRAVSHADKVRFAR"
     gene            822..1034
                     /locus_tag="VN039_13085"
     CDS             822..1034
                     /locus_tag="VN039_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46932.1"
                     /translation="MAGGESGKGLYDHLYRRFFEQRDRHDGYSFQQAPSGSAPTPVVT
                     FGEKLRWWTWDRWQRRKKILAERDRL"
     gene            1093..>1744
                     /locus_tag="VN039_13090"
     CDS             1093..>1744
                     /locus_tag="VN039_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46933.1"
                     /translation="MEKYRTCSVIRRVQTGLVALLLACLGLVTAYTPSYGVQKETQEE
                     KAANLKKQATGGLFHRWTFDQDQANTQPGSFVKGISTDEPLADWVVQDHATAPSPPNV
                     IAGSSSCTQLCYQVLLVNGLEYEYPDLSVRFHAQEGTSGLGGIVFGVRDTRNFYAAIV
                     DPSGQKVQLVRISNGGELLLAQASISLKKVDWHSLRVQRNTIISKDFIEVFADGVLV"
BASE COUNT          411 a          412 c          530 g          391 t
ORIGIN      
        1 tctcgtgcaa accgaacctt gtcggcgtgg ctgacggctc gtggggagtg ctccgccttg
       61 ccgcgcagaa ataacacgat gcagttattc acgtcgacac cggcatagtc atcaatgcct
      121 tgggcaggat cgacgatgcc atatccgacg aagacgattt gggcttgaag gtctgcggag
      181 ggcgaatcga agacggggaa ataatctgag ccaagagttt tggcggccaa atgatccgtc
      241 ggaccgactc gtaataccgg atccggtgct atcaaaggag tgggtatcag ggtggccatc
      301 atacctaatg aagcaccatc ctttcctgtc aaggaaggta ttattagtga gccattgcga
      361 acgagtggta ggcgcaagcc tgccgaaaga aactcttgtg ccacccatcg cgccgattcg
      421 aggtctgctt ttgtgccgct ttgccgccca ttaaacgagg ggccgctgag cgttcggatg
      481 tcggcaagca tccgttctgc agagagcgaa tcgatggctg tctgaaggac tgtctcaggc
      541 gattgagcca ggcttgacgt agacgtcagt gtgagaagaa ggaaggcggc accgaggacc
      601 acggtgcaac ggatcatgga atcgctgagc aaacagataa aactcatagt gataccctga
      661 tcacgtcagg tttgcattca gatgtgggat catagcatga gggaaagcag gttgcagagc
      721 tgcattgagc ccaggtacta ttcggcgggc agtaggtggc tgtgacatgt tcggataatg
      781 ttaggagcga ctgacactgt ttctggagta gaggggtgag catggctggt ggtgaatcag
      841 gaaagggact gtacgatcat ttgtatcggc gatttttcga gcaacgtgac cgccatgacg
      901 gctattcgtt tcagcaggct cccagcggca gtgccccgac accggtggtg acgttcggcg
      961 agaagctgcg ttggtggact tgggatcgat ggcaacgacg aaagaaaatc cttgccgagc
     1021 gggaccgtct gtagcgagag attcttcaaa ttaagagatc gggaagctca tagtattcag
     1081 ggactgtcag tgttggagaa atatcgaacg tgttccgtca tcaggcgagt ccagaccggg
     1141 ctcgtcgctc tcttgctggc ctgcctgggt ctcgtaacgg cgtacacccc tagttacgga
     1201 gttcagaagg aaacacagga agaaaaggct gccaatctga agaagcaagc gacgggtgga
     1261 cttttccata ggtggacctt cgaccaggat caggccaaca ctcaacccgg cagcttcgtt
     1321 aagggtattt ctactgacga gccgcttgca gattgggttg tacaggatca tgccacggcg
     1381 ccttctcccc caaacgtcat cgcgggctca tccagttgta cgcaactctg ttaccaggtg
     1441 ttgttggtca atggattgga gtacgaatat ccggatctca gcgttcgctt tcatgcgcag
     1501 gaaggaacgt ccgggctcgg aggcatcgtg tttggcgtgc gcgatacgcg caatttttac
     1561 gcggcgatcg tcgatccgag cggtcaaaag gtgcagctcg ttcgcatctc gaatggaggc
     1621 gaattactcc tcgcgcaggc gtcgattagt ctgaaaaagg ttgactggca ttcgctcagg
     1681 gtgcaacgaa ataccatcat cagcaaagat tttattgaag tgtttgcgga tggagtgctt
     1741 gtcc
//
LOCUS       DATMTI010000831         1559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_582457, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000831 DATMTI010000000
VERSION     DATMTI010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582457"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1202
                     /locus_tag="VN039_13095"
     CDS             270..1202
                     /locus_tag="VN039_13095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWV46934.1"
                     /translation="MAQKDHIRPDSRLQDWGRTLCRFGLFGSLFLSMIGLSILPASAM
                     EANFKDRAVHTISYVPQPLPWKRIPAHSILLKELRSGRILFEHEVEKRLSPASLTKIM
                     SALIILEKGRLDDLATVSRNAARAPKTHLRLKVGEVFRLGDLLKAMMMVSANDACLAA
                     VEHVGGDEEQFVTLMNAKALALGLSDTHFSNGCGFDGPDHYSTAEDLATLSEVAMRNA
                     VFRELVKGEREIITPVSGYRAYVLHNTNRLLGRIPGVEGVKTGFTSKAGRCLIAKVSQ
                     NGSDLLLVILNSNRRWNTAKSLIDYGFRLTTTVQ"
     gene            complement(1356..>1559)
                     /locus_tag="VN039_13100"
     CDS             complement(1356..>1559)
                     /locus_tag="VN039_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46935.1"
                     /translation="NNRFAYKTRGIFRNVLNSESPEDIRKLLGQEEYGDENVMGAVMA
                     YDKQGFQVNYLGKDVNIEFAAPR"
BASE COUNT          352 a          381 c          419 g          407 t
ORIGIN      
        1 gattactggc cggatgtgtc gcgttaatta tgaatcgtct tcgatagtta gagagcggga
       61 cgtgtatgca gtgtctcaag tgcagaggat tcatgatggt ggaacgccac tataccctca
      121 ttaacaggag gatatacgcc cgctgcctca attgcggatt ctggattgat ttggccgacc
      181 ttctccggtt ttttcataaa gttcttgata gtgggcggaa aggtgataaa gttcgggagt
      241 cgttcatttt ctgagagagg cggtcgtttt tggcacagaa ggaccacatc cggcctgatt
      301 ctcgtttgca ggattgggga cgaaccctct gtcgctttgg tctctttgga tcgttatttc
      361 tctcaatgat cggtttgtcg atccttcctg cctctgcaat ggaggctaac ttcaaggacc
      421 gcgccgttca caccatcagt tatgttccac aaccactccc gtggaagcga attccggccc
      481 acagtattct cctgaaggaa ttgcgatcgg gccgtatctt atttgaacac gaagttgaga
      541 aacgtctgtc accggccagt ctaaccaaga ttatgtcggc gttgatcatt cttgagaaag
      601 gccgcctgga tgatctggca acagtgagca ggaatgcggc aagagctccc aagactcacc
      661 tacgactgaa agtcggagaa gtgtttcgac tcggcgattt gctcaaggcg atgatgatgg
      721 tgtcagccaa tgacgcctgc ctggcggcgg tcgagcatgt cggaggcgat gaagagcagt
      781 ttgtgacgtt gatgaacgcc aaggcgctag cccttggatt gtcggacacg cactttagca
      841 acggatgtgg ttttgacggt cccgaccact attcgacagc tgaagatctc gcgacactca
      901 gtgaagtggc gatgcgaaac gcggtgttcc gagaactcgt caaaggggaa cgcgagatta
      961 tcacacctgt cagtggatat cgcgcctatg tcctgcataa cacgaaccgt ttactgggtc
     1021 gcatccccgg cgtggaaggg gtgaagaccg gattcacctc caaagcaggt cgatgcctga
     1081 ttgctaaagt ctctcaaaat ggtagcgatc tgctcctggt gatcctcaac tctaaccgcc
     1141 gatggaatac tgcgaaaagc ctgatcgatt acggattccg tctcacgact accgttcaat
     1201 agcagctctg ccactcatcc gtggcatttt tactcataga ccagccaagc ggtagtgtac
     1261 gtaccggtat ggtatgtcgg cctgggacag tggttctatt tcgttgtctg aaggctagat
     1321 ctgtccattg atgatatcat gtcgtcgtgg tgagactacc gtggggcggc aaactcgatg
     1381 ttgacgtctt tgccaaggta attcacctga aaaccttgct tgtcgtaagc catgacggcg
     1441 cccatgacgt tttcgtcacc atactcctcc tggcctaaca actttcggat gtcctctggg
     1501 ctttcgctgt taaggacgtt acgaaagata cctcgggtct tatatgcgaa ccggttatt
//
LOCUS       DATMTI010000832         1533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_285374, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000832 DATMTI010000000
VERSION     DATMTI010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285374"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..>1533)
                     /locus_tag="VN039_13105"
     CDS             complement(246..>1533)
                     /locus_tag="VN039_13105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV46936.1"
                     /translation="AQAGISTGESGSPVQSRTGQPLNLTLKGALAAALNNNPDVLVYK
                     ERIREAQGQVQTQLGAMLPNISADIRQTRRTQFLGTIGLSPVRTDPFSILDTRISATQ
                     NLFSLSLIQRWRASRESLHVTEQESDAKRFDTMATVALVYMEGLKAMAMVNMHEANQQ
                     VMNELLDTVRQRQHGGVATGLDVARLEAQLAAERQQGSSARYDLEHVKLSLINLLALP
                     TEIPLVLTDKWMTDVRDVPTPQGAVEEALGQRPEIQAQYTRVKASELTYSSITGERLP
                     SLVAQGDYGLVGNRWNNTLDTYNMALILQIPIFDGAQREGRIAQARSQWQQESLRMRS
                     VLNQVKMEVQDALASLTAAQEQVNIAQTGLQMATKELNLARERYTVLTSSSHFELTNG
                     LSSVARARENLVTALFQLNAARVNLARSTGSLHALN"
BASE COUNT          307 a          453 c          406 g          367 t
ORIGIN      
        1 ctctttttgc caccctattc acccgacctg aatcctatcg agcacgactt cgccgcgctc
       61 aagaaacact gggaatatca tgaaactgct tccattgccc agatcgtgaa ggcctatcaa
      121 tgattatcgg cttggctata agcgacgaca cgcccatagg cgactcgtgt ggacctcggc
      181 tcatggaatc agcggctgaa ccgccgccag tagcttgcct gttatgctcg ctccccaaac
      241 agtttttagt tcagtgcatg caaacttccc gtcgaacggg ccaaattgac cctggcggcg
      301 ttgagctgga agagcgcagt gacgagattt tccctggcac gggccaccga cgagagccca
      361 ttggtgagtt caaagtgact gctggaggta aggacggtgt agcgttctcg cgcaaggttc
      421 agttccttag tcgccatctg taaaccggtc tgtgcgatat tcacctgctc ttgcgctgca
      481 gtgagcgacg ccagggcatc ctgtacttcc attttgactt ggttgagtac cgatctcatg
      541 cgtaacgatt cttgctgcca ctggcttcgg gcttgggcga tgcggccctc acgctgggct
      601 ccatcaaaga tcgggatctg gaggatcagt gccatattgt aggtatcaag ggtgttgttc
      661 caacgattgc cgacaagtcc ataatcgccc tgggccacga gcgacggtag tcgttccccc
      721 gtgatagagg agtacgtcaa ttcggaggct ttcactcggg tgtactgtgc ctgaatctcc
      781 ggccgttggc ccaatgcctc ttccaccgcc ccctgcggcg tcggtacgtc ccgcacgtcc
      841 gtcatccatt tatcggtcaa gacaagcgga atctccgtcg gcagcgcaag tagattgatg
      901 agactcagtt tgacatgttc aagatcatac cgcgccgatg atccctgttg ccgctcagcc
      961 gctaattgcg cttccagtct ggcgacatcg agccccgtgg cgactccccc atgttgccgc
     1021 tgcctgaccg tgtcgagcaa ttcgttcatg acctgctgat tcgcctcatg catgttgacc
     1081 atggccatgg ccttcaaacc ttccatgtaa accagggcca ccgtggccat cgtgtcgaac
     1141 cgttttgcat cagattcctg ttccgtcaca tgcagagatt cgcgcgacgc tcgccagcgc
     1201 tgaatcaggc tgagactgaa cagattttgt gtcgcgctga ttctggtatc caaaatactg
     1261 aatggatcgg tacgcaccgg agacaggccg atcgttccga gaaattgcgt ccgcctggtt
     1321 tgcctgatat cggccgaaat gttgggaagc atcgcaccca attgcgtctg cacttggcct
     1381 tgggcctccc gaatccgctc cttatacaca agcacgtccg ggttattgtt gagcgccgcc
     1441 gccaacgccc ctttcaaggt aagattgagc ggctgacctg tacgtgattg aaccgggcta
     1501 ccggactctc cggttgaaat accggcctgc gcc
//
LOCUS       DATMTI010000833         5087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_115559, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000833 DATMTI010000000
VERSION     DATMTI010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115559"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VN039_13110"
     CDS             <1..343
                     /locus_tag="VN039_13110"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV46937.1"
                     /translation="DDAIDALVALGEPAVEKLIGTLRDKDENVRKFAVLALGRIKSGE
                     AIDPLITMLGDKDWFTRLTAAAALESIGDERGREAIKPLLKDPDMVVKMRVERILAKW
                     KKQPISQPANA"
     gene            complement(345..800)
                     /locus_tag="VN039_13115"
     CDS             complement(345..800)
                     /locus_tag="VN039_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46938.1"
                     /translation="MAKFLVVVLLLVGAFGAGYYLGQRPVGTLQRTVAELQQSLKDVS
                     RNVLDTTLGIERDLRRRQGLVDAKSRLVQAKAHLADRNFGDAAKELAEAVNAVEAITK
                     GAKPDNSTNALKDMTGSLREVKVEIVTGKPVSLKKLDEIQQRMDQLLNN"
     gene            complement(813..2222)
                     /locus_tag="VN039_13120"
     CDS             complement(813..2222)
                     /locus_tag="VN039_13120"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV46939.1"
                     /translation="MVTRFDAADRRLIRHLVSNPTRRVRIIGTLILLLIIAPFFTVSA
                     GTTSTVESKAAALFKDGDYPEVTALYRSLPPNAIPSKEFLRLSLLSYVRLGRTDEALA
                     IYAKLNQPGQPHDASLLRPLALGVITSHVRDRKEHVRIAAYSALAELGLPETAAILED
                     GLLDPSVVVRARAAEAIGKAGLAAKSGALRRALRDEMPTVRIAAMTALGEARATDVQQ
                     RLLNVARTEDGPEAIFASAALFKLGRAEKLDDITSATTLPDAETRMAALGVLGRLKRP
                     ASLAVLAQAVYDPDPSVRTFAAGALGEFGSPGGVAPLTHAIGDEMAMVRGVAAASLGK
                     LGIKENRPLLSALTRDPNLHVRASAAEGLIRLGDPSALTLATELARHSDPSIRGAAAQ
                     ALSASADKEALAVLQTLLQDRQPLPRLMAAKALQKGSDSAIPVLVNGLQDSDEAVRIA
                     TANSLFQQLTRRSSSKHHR"
     gene            complement(2216..4366)
                     /gene="glgP"
                     /locus_tag="VN039_13125"
     CDS             complement(2216..4366)
                     /gene="glgP"
                     /locus_tag="VN039_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWV46940.1"
                     /translation="MDSPNMVSNSLPQNLHRLPELAQNLWWSWTLEARQLFEVIDPTL
                     WFLTNHNPVKLLSDIKSDRLARLAEDPSFLRQYSAVFRLFDEYLANKKSWAGTHHADL
                     TKTTIAYFSAEFGLHASVPIYSGGLGILAGDHCKEASDLGLPLVGIGFMYPQGYFRQR
                     ITPEGWQEAAYSPFNRDESPVHLALTPSGEPCRFAVEMGSRRVIAAIWKVLVGRVPLF
                     LIDTDVPENSPEDRALSARLYGGDQEMRLCQEILLGIGGVRMLRSLGISPSVWHANEG
                     HSAFLTLERVRELVQKGSSHAEASETVRQSTVFTTHTPVPAGHDVFPHHLMDRYFSGY
                     WEQLGLSRDEFLHLGETPESSGHGFNMTALVMRLSAHVNGVSREHGRVTRQMWQHFWP
                     GLPTDQVPIRSVTNGIHAPTWISPELHHLYSKSLSPTWTHTCDDPTMWQRVMDVPDHE
                     LWAVRQTMKRKLMGFIRERARAGWMHGHLQPSQVLTRGTLLDPEALTIGFARRFATYK
                     RATLLFRDLERLKALLQDRWRPVQLIFAGKAHPADEPGRYFIHEVLNFCHDHKLGGRI
                     AFLEDYEMHMAKYLVQGVDIWLNTPRFPMEASGTSGMKAALNGALNLSVLDGWWVEGY
                     NGANGWGIQPQGEQADAQAQDHHDVEQLYRVLEQEVVPLFYQRDRDGIPRGWLQMVKE
                     CIRTVAPQFCTTRMLKDYVSLMYRAATVRLPTTW"
     gene            complement(4431..>5087)
                     /locus_tag="VN039_13130"
     CDS             complement(4431..>5087)
                     /locus_tag="VN039_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46941.1"
                     /translation="ADRLVALHIPMKDQMAFMNGLEDAVTQQLKRTLEPAFGNNADQM
                     EIVLTIGTTVAQSRQTYEKYKFLISEDSKIKNEMFRDFGDKVADALGASGNAQLSSAA
                     TLCASAVVPALSSILQGQAPPSQDASQTSPPAVGTSTEQKKSIAQEIKLVSLMSSVSG
                     EEVETRWGLHPRFREDLTSSELQQLTKLLNRVAKILGERYAAVAFSKEWASWHKAGHA
                     "
BASE COUNT         1022 a         1462 c         1522 g         1081 t
ORIGIN      
        1 agatgatgcc attgatgctc tggtcgcatt gggtgagccg gccgtcgaaa agttgatcgg
       61 aactcttcgc gacaaagatg aaaacgttcg taaatttgcc gtcctcgcac tgggacggat
      121 taagagcggc gaggccattg atccgctcat tacgatgctc ggtgataaag attggtttac
      181 acggttgacg gccgccgccg cgttggaatc gatcggtgac gaacgggggc gcgaggcgat
      241 caaaccgttg ctcaaagacc ccgatatggt cgtgaagatg cgggtagagc gaattctggc
      301 aaaatggaag aagcagccga tctctcaacc ggccaacgcc tgagtcagtt attcagcagt
      361 tgatccattc tctgctggat ctcatccagc tttttcaacg acaccggttt ccccgtcaca
      421 atttccacct ttacctcccg cagcgatccg gtcatgtcct ttagggcatt ggtcgaatta
      481 tcgggcttgg ctccctttgt gatggcttcc acggcattga cggcctcggc caattctttc
      541 gctgcgtcgc cgaaattgcg atcggcgagg tgtgccttcg cctggaccaa acgggatttg
      601 gcgtccacga gcccctgacg tcggcggaga tcgcgctcga tcccgagtgt cgtgtccaac
      661 acgtttctgg acacatcctt caacgactgt tgaagttccg cgaccgtccg ctgcagcgtt
      721 ccgacgggcc gctgtccgag atagtagccc gctccgaatg ccccgacgag cagcaacaca
      781 acaacgagaa atttagccat agaggtcctt ggttaacggt ggtgtttcga cgaactccgt
      841 cgggtcaact gctggaacag gctgttggcc gtcgcgatac gcacggcctc atccgagtct
      901 tgcaagccat tcactaagac ggggatggcg ctatcactgc ctttctgcaa cgctttggcg
      961 gccatcaggc ggggaagcgg ctgccggtcc tgtaagagag tttgtagcac cgccagagct
     1021 tctttgtcgg cagaggcgct caaagcctgc gccgccgcac cgcgaatgga agggtcggaa
     1081 tgccgagcca gctcggttgc cagcgtgagg gcggagggat cgccgaggcg aatcaaccct
     1141 tctgcggcgc tggcacggac atgaagattc ggatctctgg tgagtgccga cagcaagggc
     1201 cggttttcct tgatgccgag cttcccgagg cttgccgcgg caacgccgcg aaccatcgcc
     1261 atctcgtccc cgattgcatg tgtcagcgga gccacgccgc ccggggaccc gaattctccc
     1321 agtgctccgg cagcgaacgt gcgcaccgat ggatccggat catacacagc ttgagccagg
     1381 actgccaggc ttgccggacg tttgagacgc cccaatacac ccagtgcggc cattctcgtc
     1441 tctgcgtccg gcaaggtggt tgcgctggta atatcgtcca gtttttcagc acggcccagc
     1501 ttaaacaggg ctgcagaggc gaagatggcc tcaggcccat cttctgtgcg ggcgacgttg
     1561 agcaatcgtt gttggacgtc ggtggcacgt gcttctccca gggcggtcat ggcggcgatg
     1621 cggacggtcg gcatttcgtc ccgcaacgct cgacgcaagg cgccggactt tgccgccagt
     1681 ccggcttttc cgatcgcctc agcggctcgt gctcgtacga cgaccgaagg atcgagcaag
     1741 ccatcctcta aaatcgcggc ggtctccggc agtcccagtt ccgccagggc cgagtaggcc
     1801 gcgatgcgga catgctcttt gcgatcgcga acatggctgg tgatcactcc gagtgccaat
     1861 ggccgaagaa gcgacgcatc atgaggctgc ccaggctggt tcaatttcgc atagatggcc
     1921 agggcttcgt ccgttcgtcc aagacgaacg tagctcagca aggagagtcg aagaaattct
     1981 tttgacggta tcgcgttcgg tggaagactt cggtagagag cggtcacttc ggggtagtca
     2041 ccgtccttga agagggccgc agctttcgat tcgaccgtgg aagtggttcc tgccgataca
     2101 gtaaagaacg gcgcgatgat gagaagtaga atcagcgtgc cgatgatacg cactcttcta
     2161 gtcggattgg agaccaggtg gcgtatcaga cggcgatccg ctgcgtcgaa ccgagttacc
     2221 atgtcgtggg cagccgcacc gtggcggcgc ggtacatcaa gctcacataa tccttcagca
     2281 tgcgtgtggt gcagaactgg ggcgcgacgg tacggataca ttctttgacc atttggagcc
     2341 agcctcgggg aattccgtca cgatcgcgct gatagaacag cggcacgact tcctgttcca
     2401 gcacacgata gagttgttcc acatcatgat gatcttgggc ctgtgcgtcg gcctgttcgc
     2461 cttgcggttg gattccccag ccgttggccc cgttatagcc ttcaacccac catccatcga
     2521 ggacactgag gttcaacgcg ccgttcaggg cggctttcat gccgctggtg ccgctggcct
     2581 ccattggaaa gcgaggagtg ttcaaccaaa tgtcgacgcc ctggacgaga tatttcgcca
     2641 tatgcatctc gtaatcttcc aagaatgcaa tgcggcctcc caacttgtgg tcatggcaga
     2701 agttcagcac ctcatgaatg aagtatcgac caggctcatc ggctggatgg gccttgccgg
     2761 cgaagatgag ttggaccggc cgccagcggt cttggaggag ggccttcagc cgctccaggt
     2821 ctcggaaaag caaggtggcc cgcttatacg tcgcgaaccg tcgggcaaac ccgatcgtca
     2881 aggcttcagg gtcgaggagt gtgccgcgtg tgaggacttg agagggttgc agatggccgt
     2941 gcatccagcc tgccctggct cgttcacgga taaatcccat cagtttccgc ttcatcgttt
     3001 gtcgaaccgc ccacagctca tggtcgggga catccattac ccgctgccac atggtcggat
     3061 catcgcaggt gtgggtccag gtcgggctga gcgacttgct gtacagatgg tgcagttccg
     3121 gcgagatcca ggtcggcgca tgaatgccgt tcgtcacgct ccggatgggg acctggtcgg
     3181 tcggcagtcc cggccagaag tgctgccaca tctggcgagt cacgcggcca tgttctcggc
     3241 tgacgccgtt gacgtgggcc gacaggcgca tgaccagcgc ggtcatgttg aaaccgtgcc
     3301 cgctcgattc cggagtttcg ccgaggtgca gaaattcatc gcgcgagagt cccaattgtt
     3361 cccagtaacc ggaaaaatat cgatccatca gatggtgcgg gaacacgtcg tgcccggccg
     3421 gcacgggtgt gtgcgtcgtg aacacagtgc tctggcgaac cgtctcgctg gcttcggcgt
     3481 gggaggaacc tttttgaacc agttcccgca ccctctccaa ggtaagaaaa gccgagtgac
     3541 cttcgttggc atgccagacc gacggcgaga tgccgagcga acgcaacatg cgcacgccgc
     3601 cgatccccag caaaatttcc tggcagagcc gcatttcttg atccccgcca tagagacggg
     3661 cggagagggc acggtcctcc gggctgttct ccggcacatc cgtatcgatg agaaagagcg
     3721 ggacccgtcc caccagcact ttccagatgg ccgcgatcac acggcggctg cccatttcaa
     3781 cggcaaaccg gcagggctct cccgacggag tcagagccag atgaaccgga gattcgtctc
     3841 ggttgaaagg ggagtaggcc gcctcttgcc aaccttccgg cgtgatccgc tggcggaaat
     3901 agccttgcgg gtacatgaat ccgatgccga cgagcggcaa gcccaggtcg ctcgcctctt
     3961 tgcagtggtc cccggctaag attccgaggc caccgctata aatgggcacg gaggcatgca
     4021 acccgaattc tgccgagaag tatgcgatgg tggtcttcgt caaatcggca tggtgcgttc
     4081 cagcccaact cttcttattg gccaggtatt catcgaacag tcggaacacc gccgagtatt
     4141 ggcggagaaa tgaaggatct tcagccaaac gcgccaaccg atccgatttg atatcagaca
     4201 agagtttgac cggattgtgg ttcgtgagaa accacagggt cggatcgatc acttcgaaca
     4261 gctggcgcgc ttccaatgtc caactccacc agagattctg cgccagttcc ggcagacggt
     4321 ggagattctg cgggagcgaa ttactcacca tattaggaga atccacatct actcctttgc
     4381 tcaaaatcac gcattcggta tcggcaagga tgggcgatca ccggctgaga tcaggcgtgc
     4441 cctgccttat gccacgaggc ccactctttg gagaatgcca cggcggcgta ccgttcgcca
     4501 aggatttttg cgacccggtt gaggagttta gtgagttgct ggagttcgga tgaggtgagg
     4561 tcctcacgga accgcgggtg gagtccccag cgggtctcaa cctcttcacc ggatacgctc
     4621 gacatgagac ttacgagctt gatctcttgg gcgatcgatt ttttctgttc ggttgaggtc
     4681 ccgacagctg gaggcgaagt ctgggatgcg tcctgagagg gaggagcctg tccttgcagg
     4741 atcgagctga gggccggcac gacggcactg gcgcatagcg ttgccgcaga gctgagctgt
     4801 gcattgccgg aagcgccaag cgcatcggct actttgtcgc cgaagtcgcg gaacatctca
     4861 ttcttgatct tggagtcttc tgaaatcagg aacttgtatt tctcataggt ctgtcgactt
     4921 tgcgccacgg ttgtgccgat cgtcagcacg atctccatct gatccgcatt gtttccgaat
     4981 gccggctcca atgtacgctt caattgttgg gtcacggcgt cttccaaccc gttcatgaat
     5041 gccatctggt ccttcatcgg gatgtgcaga gccaccagcc gatccgc
//
LOCUS       DATMTI010000834         1993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_625102, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000834 DATMTI010000000
VERSION     DATMTI010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625102"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(886..1458)
                     /gene="nuoI"
                     /locus_tag="VN039_13135"
     CDS             complement(886..1458)
                     /gene="nuoI"
                     /locus_tag="VN039_13135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWV46942.1"
                     /translation="MRLRERLARQYRGFTAWIKLITFYELAVGMKSTLSHLIHYKPIT
                     LQYPHEKRILPDNYRGMLALLRYADGTEKCVGCDLCEAACPSRVIAVVSAEVPEEPTK
                     RYAREYSMDMTRCLFCGLCVQACPVDALAMTKEYEWAVYDKRDLFLNKQQLLAIGDRT
                     FQTREKRLEFQHPNLAVFNIASSNHPSKAC"
     gene            complement(1455..>1993)
                     /locus_tag="VN039_13140"
     CDS             complement(1455..>1993)
                     /locus_tag="VN039_13140"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HWV46943.1"
                     /translation="IGLMEALDGLGYVTTGVSFLAVSMVLFVRAWYVFAISVGSDAVL
                     AVLGLVHDLLLVIILLELFRTTINFLKTKVITLEPFLYICVIASTRRILTTGAQVSYM
                     DELTDLVFNRYLMDLGANVLVVVALIIAIYVSRRASPPVMAEGDETAQCTDRTGERAE
                     SCPQETDEQIVRLKKEYA"
BASE COUNT          502 a          608 c          448 g          435 t
ORIGIN      
        1 cgcacactga cgtttgaaaa caaacgcccg acctcgctct actattaccg tgagcttcag
       61 agcctcagag acccgctgca ttttcagacc tctagcagga aacttcagca aggtaacaac
      121 gcattgacag cttattaatc cgtcagcagg agagtcacga acagtgcaac cacccatgcc
      181 atcgcatacg caagcaagaa gagttccgct gccatctcga tccgaaaatc acgccacctg
      241 ctcataactt cctcccggtg ttaatccgcc gatttaggcg ctgaacgaac tgccgcatcc
      301 gcacgtgatc tttacggtcg gattcttcat tacaaaccct gagccctgcg ggtggtccac
      361 ataatcaact tccgtaccga ccagcaacgg agcgctctgc gaatccatga cgaccttgac
      421 ggtccgtcag caactagctc ttcacccaca tgcttgtttc tctttcaacg acttgactcc
      481 atcacccctt tactcactcc gacgaccgcg cggtttcact acacacgacc tttcccaggc
      541 ctagctgcag atgacccagg cgggcgtgca cggttccgaa tcggttggct ctgccttact
      601 tgattagcct cgatccctgg gtcagtcgtc gcaccacaga gtaggcaatg ccatccagat
      661 accgggggct gccccatgtc tctcatttgg acggcaatca aaaggccttg gcagcgtaga
      721 cagcgcatga tgtctcctcg ggtttctcta caacatcctt ctcaaggtcc cgttccagat
      781 aagggatgca tacatggaat cggaggaact tcgatagaag caataccacc atgcctctgc
      841 gatgaaagcc cacagattga tcatgtcagg cctccttcca ggcaactaac acgctttgga
      901 tggatggttc gatgaggcaa tattgaagac cgccagattc ggatgttgga actccagccg
      961 tttctcgcgt gtctgaaacg tacgatcccc aatcgccaac agctgttgtt tattgaggaa
     1021 cagatcccgt ttgtcataga ccgcccactc atattccttc gtcatcgcaa gggcatcgac
     1081 cggacaggct tgaacacaca gtccacaaaa caaacatcgc gtcatatcca tcgaatattc
     1141 tcgtgcgtat cgcttcgtcg gctcctccgg cacttcggcg ctcacaactg caatgacacg
     1201 agagggacag gctgcctcac agaggtcgca cccgacacac ttctcggttc catcggcata
     1261 acgcagcaat gccaacatac cgcgataatt gtccggcaga atccgcttct catgcggata
     1321 ctgaagagtg atcggcttgt agtgaatgag atggctcagg gttgacttca tacccacagc
     1381 aagctcgtag aaggtgatca gcttgatcca agccgtaaac ccacgatact gccttgccaa
     1441 tctctccctc agcctcatgc atattctttc ttgagcctga cgatctgttc atccgtctct
     1501 tgcggacacg actctgctct ctcgcccgtc cgatcagtgc actgagccgt ctcatcccct
     1561 tcagccataa ccggcgggga tgcccgtcgc gagacataga tcgcaatgat caaagccacc
     1621 acgacaagca cattcgcccc aagatccatg agatatcggt tgaacacgag atcggttagt
     1681 tcgtccatat acgaaacttg ggctcccgtg gtaaggatgc gccgcgttga cgcaatcacg
     1741 cagatataca agaacggttc caaggtaatc actttggtct tgagaaaatt aatggtagtc
     1801 ctgaacagct ccaacaagat gatgaccaat aagagatcat gcaccagccc gaggactgca
     1861 agaaccgcat ccgatcccac tgagatcgcg aagacgtacc aggcacggac aaacagcacc
     1921 atactgacgg ccaggaaact cactccggtc gtgacataac ctaacccgtc aagggcttcc
     1981 attaggccga tcg
//
LOCUS       DATMTI010000835         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_73132, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000835 DATMTI010000000
VERSION     DATMTI010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73132"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..378)
                     /locus_tag="VN039_13145"
     CDS             complement(19..378)
                     /locus_tag="VN039_13145"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV46944.1"
                     /translation="MAALSERRKFVRATLVGSALVAPKSGGRACTAVLDNVNKLGAGL
                     HTKDRFPPNEKVTVSLAFLDSDRIEQQEKLDGTVAWVRPWDKKGFLIGVVWDELVTKE
                     KNRWLYYYLEETLKASV"
     gene            complement(462..1760)
                     /locus_tag="VN039_13150"
     CDS             complement(462..1760)
                     /locus_tag="VN039_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWV46945.1"
                     /translation="MARSRDQAPDLFTLPEGSQDTVEAPLAERMRPHEFADFVGQDEI
                     VGPDCPLRKAIEADRLSSVIFWGPPGSGKTTLAHLIARHTKAQFVHFSAVTSGVPELR
                     MIIKAAEHRRAGNGQRTILFVDEIHRFNKAQQDAFLPHVERGTIILVGATTENPSFEV
                     ISPLLSRSLVVVLKPLTEEALGQILDRALTDVERGLGKWKARFLPDARQRLISFGNGD
                     ARALLTSLEFVVTQASAGPDGTRAIDAVSLESALGKKALRYDKAGEEHYNVISAYIKS
                     LRDSDANGALYWLARMLEAGEDPKFIARRMVIFASEDIGNADPLALLVATSVAQAVQF
                     VGLPEAQINLAQGTTYLASRPKDNASYVGLLEALKDTKTHGNLAVPLHLRNAVTSLMR
                     DQGYGADYRYVHDDPRARTEQTHLPATLKDRRYYRPRPPE"
     gene            1872..>2437
                     /locus_tag="VN039_13155"
     CDS             1872..>2437
                     /locus_tag="VN039_13155"
                     /inference="COORDINATES: protein motif:HMM:NF015081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV46946.1"
                     /translation="MQARINNITFAFNDQGTGLPIIFLHGFPLNRTMWAKQEEALSSQ
                     FRVITIDLRGHGESDAPLWHYSLDQAADDVHGLLDHLSIRQAVFVGLSMGGYILFAFY
                     RKYAEYVKGLVLADTKAQADTDEGKQARFEMAQIAYKQGARAIADIMIPKLLSPATIQ
                     TRPELVQRVRTMIEGNQVSGIAGDLMAMA"
BASE COUNT          533 a          699 c          678 g          527 t
ORIGIN      
        1 gggcttttca acgccctatt agacggaggc tttgagcgtt tcttccagat agtagtacaa
       61 ccagcggttc ttctctttcg tcaccaattc gtcccagacc accccgatca agaatccttt
      121 cttgtcccag ggcctgaccc aggcgacggt cccatcaagc ttttcctgtt gttcgattcg
      181 atcggaatcg agaaacgcaa gggacacggt gactttttca ttcggaggga accgatcttt
      241 ggtatgaagc ccggccccga gtttgttcac attatccagg accgctgtac aagcccgccc
      301 gccgctcttc ggcgccacca aggcggaacc gaccaacgtg gctcggacaa atttcctccg
      361 ctcactcagt gcagccatgc cggctgacgg ttttccttcc cctcgtttca ttgttctaag
      421 tataaccgat tagacaagaa tgtaaactag gataattggc cctattccgg cggcctaggg
      481 cggtaatacc gtcgatcttt cagtgtcgcc ggcagatggg tttgttcggt tcgtgctcgt
      541 ggatcgtcat ggacatagcg gtagtcggcc ccatagcctt ggtccctcat cagggaagtc
      601 acggcatttc ggagatggag cggaactgcc agattaccgt gggttttcgt atctttcagg
      661 gcttccaaga gaccgacgta ggaggcgttg tccttgggtc gtgatgccag gtaggtggtg
      721 ccctgagcca gattgatctg tgcctccggg agtcctacga actggactgc ttgcgccacg
      781 gatgtggcca cgagcagggc caatggatcg gcattgccga tatcctcgga cgcgaaaatc
      841 accatccgtc tggcgatgaa tttcggatct tcgccggcct ccagcatccg ggccagccag
      901 tagagcgccc cattcgcatc agaatcgcga agactcttga tgtaggctga gatgacgttg
      961 taatgctctt cgcctgcctt gtcgtaccgc agcgccttct tgcctagcgc tgattccaac
     1021 gaaaccgcat caatcgcgcg agtgccgtca ggtccggccg atgcttgggt caccacaaac
     1081 tccaaggatg tgagcagcgc ccgcgcatca ccattgccga acgagatcag gcgttgccga
     1141 gcatccggta agaagcgcgc cttccatttt cctagtcctc gctcgacgtc ggtcagcgcg
     1201 cgatcgagaa tctgacccaa cgcctcctca gtaagcggct tgaggaccac aaccaatgac
     1261 cgcgacagta gcgggctgat gacctcaaag gaaggattct ccgtggtggc cccgaccaga
     1321 ataatggtgc ctcgttcgac atggggaaga aaggcatctt gctgcgcctt gttgaagcga
     1381 tggatctcat ccacaaagag aatagtccgc tgaccattac ccgctcggcg gtgctcggcc
     1441 gccttgatga tcatgcgcaa ttccggcacg ccgctggtca cagccgagaa gtgaacaaac
     1501 tgggccttgg tatgccgggc aatgagatgg gccagagtcg tcttccccga accgggtggt
     1561 ccccagaaga tgacggaaga aagccggtcg gcttcaatcg cctttcgcaa ggggcaatcc
     1621 gggccgacaa tctcgtcttg cccgacgaaa tcggcaaact catgcggtct catccgctcg
     1681 gcaagcgggg cctcaaccgt atcttgggag ccttcgggga gggtgaacag atcaggggcc
     1741 tgatcgcgag agcgggccat tgcactcctc gagaatagcg ccaattgtat acgtcgttcc
     1801 tcaggatcgc aaacgccctc ttgaccatac agacaaccga tggtacgaac acggcagtta
     1861 cggagccgac cgtgcaagcc cggattaaca acatcacctt cgcgttcaac gatcagggga
     1921 caggccttcc catcatcttt ctccatggct ttccactcaa tcgaacgatg tgggcaaaac
     1981 aggaggaagc gctgtcgtca cagttccgag tgattacgat cgacttacgc gggcatggtg
     2041 aatccgatgc gccgctctgg cactattccc tcgaccaagc agccgatgat gtccatggtc
     2101 tgcttgatca tctgtcgatc cggcaagcgg tctttgtcgg actttccatg ggagggtaca
     2161 tcctgttcgc gttctatcga aagtatgccg agtatgtcaa aggactagtg ttggccgaca
     2221 cgaaagcaca agcggatacc gatgaaggga agcaggctcg gtttgagatg gctcagattg
     2281 cgtataaaca aggggctcgt gcgatcgccg acatcatgat tcccaagctg ctcagtcccg
     2341 caacaatcca aacgagaccg gagctggttc aacgcgtgcg cacgatgatc gagggcaacc
     2401 aagtcagcgg catagcggga gacttaatgg cgatggc
//
LOCUS       DATMTI010000836         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_498733, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000836 DATMTI010000000
VERSION     DATMTI010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498733"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..386
                     /locus_tag="VN039_13160"
     CDS             111..386
                     /locus_tag="VN039_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46947.1"
                     /translation="MEYDLSPKALDALSQGDRSKAIEQIQLERNMSREEARELVAAFI
                     LSQPSLQAKMRMEENRAETQWGLMRWLILFQAIAVAVGYFLFFRDQW"
     gene            716..>2316
                     /locus_tag="VN039_13165"
     CDS             716..>2316
                     /locus_tag="VN039_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWV46948.1"
                     /translation="MKRALAWYAWSAEALADELRTDPEAGLNVDEAARRRTQEGPNEL
                     PEAPPPSLLKLFVSQFSSFLVWVLIGAAIVSGLLEDWIDAAAILAIVFLNGVLGFVQE
                     FRAERSLAALRKMSVATARVVREGRLQSIPARELVRGDLIALEAGDRIPADSRLLYAT
                     NFQAQEASLTGESTPVQKQAELLDTTEVPLADQRNMVFMGTVAVSGKARGLVVGTGLG
                     TELGRIAAMIRKADEAERAETPLQRRLEQFGYTLLWLALGVVTVVFALGSLRGEPLVA
                     MFLISVSLAVAAVPEGLPAVVTITLALGVTRMAKRHALIRKLPAVETLGSATVICTDK
                     TGTLTKNEMTVMRVFMGDSHVEVTSEGYEPKEEIRKSSAVLKVLSPESSDRPGRSFPE
                     NSARSTQHPGLPSGLRDLLTAAVLCNGATLQQENGIWRIIGDPTEGSLLVAAAKAGLT
                     KAKLERRAPMEREVTFDAERKMMTIIRRTEQGRTAYCKGAPDVLLKRCAGRVTLDGRT
                     EALDDEHRQRIGEANASLAQQALRVL"
BASE COUNT          512 a          636 c          705 g          463 t
ORIGIN      
        1 tctgctcaac gcatcgatgc gctttaaacg aagtcaccgt ggaaatcagg aagacgggca
       61 gcggtctttt ctggtaaact ctaccggtca caatgacgga ggaatcacga gtggaatacg
      121 atctttcccc aaaagctctg gacgcactgt cgcaaggcga ccgcagcaaa gcgatcgaac
      181 agattcagct ggagagaaac atgagccggg aagaggcgag ggagctggtt gccgcgttta
      241 tcctctcgca accttctctg caagcgaaaa tgagaatgga ggaaaaccga gcggagacgc
      301 aatggggtct catgcgctgg ctgatcctgt tccaagcgat cgcggtcgca gtcgggtact
      361 tcctcttctt ccgggaccaa tggtgagcgg acctgccaca agcagccgca cgagttaccg
      421 ttcccgaccc cggtggcaac ttttcctccg ttgagaagcg ctcacttcca ggcttctcct
      481 tcggtggcta ataaaaagca aaccaccacc tcctccttaa taatgtcctc aagaccctcc
      541 ttctttgtgt tgcattcgac cactaaggct ctccgctaac gtggcatgac gccacagtga
      601 acagggtaag atgcctgaga gtcgatacta tgggatggca tgcaccctgc tgcgaattcc
      661 ccctagcgaa agggacgtta tggggattga ggggagaata caggcatcca atgagatgaa
      721 gagagcacta gcatggtatg cctggtcagc agaggcactg gctgatgaac tccggacaga
      781 ccccgaagcc ggtttgaatg tcgatgaagc ggcacgtcgg cgaacgcaag aaggccccaa
      841 cgaattacct gaagctcctc ctccctcgct gctgaaactc tttgtctcgc agttctcgag
      901 ctttctcgtg tgggtgctga tcggagcggc cattgtgtcc ggtttgctgg aagactggat
      961 cgatgcggcg gcgattctgg cgatcgtgtt tctcaacggc gtattggggt ttgtgcagga
     1021 attccgagcc gagcgatcac tggcggcact gcgcaagatg tcggtcgcga cggcccgtgt
     1081 cgttcgcgag ggcaggctgc agtccattcc ggcacgagaa ctggtcagag gagacctaat
     1141 cgccctcgaa gcgggcgacc gtattcctgc ggattcacgt ttgctctacg caacgaattt
     1201 ccaagctcaa gaagcctcac tcaccggtga atcgacgccc gtacaaaaac aggcggagct
     1261 gctcgatacc acagaggtac cgctcgccga tcaacgcaac atggtcttca tgggcaccgt
     1321 ggccgtctcc ggcaaggccc gtgggctggt tgtgggaact ggtcttggaa cggaactggg
     1381 ccggatcgcc gccatgatcc gcaaggccga tgaggcggag cgcgccgaaa caccgctgca
     1441 acgacgattg gaacaattcg gctatacact cttgtggctg gcgcttgggg tggtcacagt
     1501 cgtgttcgct ctcgggtctc tccgagggga accgctggtt gcgatgtttc tcatatcggt
     1561 gagcctcgct gtggcggctg tgcccgaggg tcttcccgct gtcgtcacga ttacactggc
     1621 tttgggcgtg accaggatgg ccaagcggca tgcgttgatt cgtaagctgc ccgccgtcga
     1681 aacgctgggt tccgccaccg tgatctgtac cgacaagacc ggcaccttga cgaagaatga
     1741 gatgacggtg atgcgtgtgt tcatgggaga ctctcacgtc gaagtgacca gcgaagggta
     1801 tgaaccgaaa gaggagattc ggaaaagcag tgctgtgtta aaagtgctga gtcctgagtc
     1861 atcggatcga ccaggacgtt cttttccgga aaactcagca cgcagcactc agcacccagg
     1921 acttccctct ggtctgcgcg accttctgac tgccgccgtg ctgtgcaacg gtgcgacatt
     1981 gcaacaagag aacgggatct ggcggatcat cggtgatccg accgaaggtt cgctgctcgt
     2041 ggcggcggcg aaggccggcc tcacgaaggc taagttggag cgtcgggcgc cgatggaaag
     2101 agaagttacg ttcgacgccg aacggaagat gatgacgatc atccggcgaa cggagcaagg
     2161 ccgcacggct tactgcaaag gggcgcccga tgtcttgctg aaacgttgcg ccggacgagt
     2221 cacgctggat ggccggaccg aggcactgga cgacgagcat cggcaacgga tcggcgaagc
     2281 caatgcttcg ttggcacaac aagccctacg ggtact
//
LOCUS       DATMTI010000837         4426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_838868, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000837 DATMTI010000000
VERSION     DATMTI010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838868"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VN039_13170"
     CDS             <1..313
                     /locus_tag="VN039_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46949.1"
                     /translation="PYSSRVQPLCLSVILGLAIDRRTKTTFQDGTSTNYTYDGGNRIT
                     QVQEKNAGGTVTDTITRTYDDLDRLTQEVTSEKTVNYTYDTASRRATMSVSPTNKAQR
                     R"
     gene            complement(530..2770)
                     /locus_tag="VN039_13175"
     CDS             complement(530..2770)
                     /locus_tag="VN039_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWV46950.1"
                     /translation="MNRWKLYKRILRTLLVVVPFLAASSIQDAAGYLSRTPTAFSEDG
                     RILLVQMEELVVWDLETKALVAKIPGLHCEQVALLKQEGWVLCVEHSVTIYDWKNRVS
                     VATIPPESQQPDSLLAYSKETDRMILRHGNEAVSVWQLGKKPVPIKHISLEGKTDISS
                     VAASPDTKLLAIAQGHTIHLHDLNGTTVRDIVIEGGKPHDLLFAPNSSRLAGSSGKMI
                     FLIDPVDASIAVRATVTSAERAQGSLTPEVFSRDGHRLVAANGEWGYALFKTDTGELV
                     SLTEFTYAAPGRGMRSPTQLRAVDIAADADLLVGQPEHLHTLQIWDLQTGAMLPDLCG
                     EDCRNMGLRVSLLKWSPIGSKIVVGMQGALNPDVDGKISIWDIPSRSPELVLDPGQPQ
                     AKVLAKRATPPTFITMTSSPPAAPAFAHARALRAVATSPSANLLVTSGDDGLLKIWDP
                     GQGALLRQLVLTAPAHALAFSADGAILAAGTMEGDVRLWETQTWREFSPYSSRQGRIN
                     ALQFIPGNRFLVVAGGEPRVLVMDLVTRAVVRELVHTSRYPACDEKRCIKKRAVRREA
                     VDSLSFLDGSSFLLTTSQSGSVVWDITTWREVEKPAGLPDAWSGLGWKRPFVWMTTRA
                     GVPNAFTLAVWDSKNNKVLASLDTFTHRDTEIDHGPAVALGTTMAVDPSHRWAATRVG
                     EHISVWDLSAQTKRKTFHVKMPYHLHWTSDGNYLIVATLDRKILVWSAETMEPAHYLR
                     DLSVTR"
     gene            complement(2781..3332)
                     /locus_tag="VN039_13180"
     CDS             complement(2781..3332)
                     /locus_tag="VN039_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWV46951.1"
                     /translation="MKVLTLGLMSGLLFWPVIGSAELYKWTDDQGNLHITDMPPPSTQ
                     KKSATTVTPVPRSASPKKVTVQPTLPGRPQAEIHPVPGPSGPSPATEEVLIPRPMEGL
                     KPNQATLTSLWEIFDSTRMNAKAPVQRWKDEQGLDHFVDVLPATLSRSEVAPKGEDVS
                     ASPTGRTKERVTGVSRSRHQSTK"
     gene            3800..4318
                     /locus_tag="VN039_13185"
     CDS             3800..4318
                     /locus_tag="VN039_13185"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWV46952.1"
                     /translation="MAQALTSEDVQQAWSALTEDTRIAVLLTMRNGRPFGSHVPYVFG
                     SDWTKAYLHLSRLALHTQHLLADPHVSLFIAETDGPEKNPLALRRINLMGQAAPLSPD
                     TPSYGELKERYLSRFPQAEMMFGFGDFALWELRLEDAHFVLGFGQAFVSTVATPSRWV
                     HQKVERPTARKD"
BASE COUNT         1035 a         1260 c         1186 g          945 t
ORIGIN      
        1 gccctattct tccagagtac aacccctctg tctatcagta atcctgggct tagctataga
       61 ccgaagaacg aagaccacct ttcaggacgg cacgtccacg aactacacct atgatggcgg
      121 caatcgcatc acccaggtgc aggagaagaa tgcgggcggc accgtcacag acacgatcac
      181 gagaacctat gatgacctcg atcgactcac ccaggaagtc acttctgaga agacggtgaa
      241 ctacacctac gatactgcca gccgccgagc gacgatgtcc gtctcaccta caaacaaggc
      301 gcaacgacgt tgaggaccta caggtatgaa gccgtgggca atcggatcaa gacgggcgat
      361 acctttgcgc gaagaaatct accagccgct ttcaccagca caagctacaa catcagtaat
      421 cagcagacca tgttcggcac aagtacagaa gctgaatgac aatctgactc cagggcagat
      481 gccgttcctg agccatccgc atagagcaat tgtaatggtt tatgacgtat cagcgggtaa
      541 ctgacagatc tcgcaggtag tgcgccggct ccatggtttc ggctgaccag acgaggattt
      601 tacggtcgag agtggccacg atcaaatagt tgccgtcgct tgtccaatga agatggtacg
      661 gcattttgac gtggaacgtc tttcgttttg tttgtgccga aagatcccaa actgaaatat
      721 gttctccgac tctggtggca gcccatcgat gcgatgggtc gacggccatg gttgtcccta
      781 acgccacggc cggcccatga tcaatttctg tatcccggtg ggtaaaagta tccaagctcg
      841 ccaacacttt gttgttctta ctgtcccaga cagcgagggt aaaggcgttc ggaacaccgg
      901 cgcgagtggt catccagaca aatggacgtt tccagccaag accggaccag gcatccggga
      961 gaccggccgg cttctcaacc tcgcgccagg ttgtgatgtc ccagacgaca gaaccggatt
     1021 gcgaggtcgt caaaagaaac gagcttccat ctaagaagct caaactgtct acggcctctc
     1081 ttcgtaccgc tcgctttttt atacagcgtt tctcgtcgca ggccggataa cggctggtat
     1141 gcacaagctc tctcacgacc gctcgggtga cgagatccat gacaagaact ctcggttctc
     1201 ctccggcaac gaccagaaat cgattgccag gaatgaattg cagcgcgttg atccgacctt
     1261 gcctgcttga atagggtgag aactcacgcc aagtctgggt ttcccacaaa cgtacgtctc
     1321 cttccatggt accagccgcc aagatcgcgc catcggcact gaacgcaagc gcatgtgccg
     1381 gagcagtcaa taccagttga cgcaagagag ctccctgccc tgggtcccat attttcagca
     1441 atccatcgtc gccgctcgtg acgagcagat tcgcgctagg agacgtcgcc actgcgcgca
     1501 aagcccgtgc atgggcgaaa gccggcgcag ccgggggaga agatgtcatc gtaataaatg
     1561 ttggtggggt cgctcgtttg gccagtacct tggcctgagg ttggccgggg tccagcacca
     1621 attccgggga acgtgaggga atgtcccaaa tcgatatctt cccatccaca tcaggattga
     1681 gggctccctg catcccaacc acgattttcg aaccaatcgg cgaccatttg agtaacgaaa
     1741 cgcggagacc catattgcgg caatcctctc cgcacagatc cggcaacatc gccccggtct
     1801 gtagatccca gatctgtaat gtgtgaagat gctccggttg tccgaccaag agatctgcat
     1861 cggcagcaat gtcgacggca cgaagctgtg tcggtgagcg catcccgcgc cctggagctg
     1921 cataggtaaa ctcggtcaaa gacaccagct cgcccgtatc agtcttgaaa agcgcatagc
     1981 cccattcccc gttggctgcc accaaacgat gaccgtcccg cgagaagact tcgggcgtga
     2041 gggagccctg tgcccgctca gcactcgtca ctgttgcacg cacggctatt gacgcatcca
     2101 ccggatcgat caggaaaatc attttgcccg aactccccgc caaacgagaa ctgttcggcg
     2161 caaagaggag atcatgcggt ttgccgcctt ctataactat gtcacggacg gtggtcccgt
     2221 tgaggtcgtg cagatgaatg gtgtggcctt gcgcaatcgc caacagcttc gtatcgggcg
     2281 aggcggccac ggaagagatg tctgtcttcc cctccagcga aatatgtttg atcggtacgg
     2341 gctttttccc cagctgccag acggacactg cctcattccc gtggcggaga atcatccgat
     2401 cggtctcttt cgaataggca agcaggctat cgggctgctg cgattccggt ggaatggtgg
     2461 ccactgagac tcggttcttc caatcgtaga tggtgacgct gtgctccaca cacaacaccc
     2521 acccctcctg cttcagcaac gcgacctgct cgcagtgaag acctggaatc ttcgccacga
     2581 gcgccttcgt ctcaagatcc cagacaacca gctcctccat ctggaccagt aggatccgcc
     2641 catcttcaga gaatgcagtg ggagttctag agagataacc agccgcgtcc tgaatcgatg
     2701 aggcggcaag gaaggggacg acaacgagta gggtcctcag tatcctcttg tacagtttcc
     2761 aacgattcat ggtcttcgct tcatttagtc gattgatggc gcgaacgaga tacaccggtc
     2821 acccgctcct tggtcctacc tgtaggggag gcagaaacgt cttcaccctt tggcgcaact
     2881 tctgagcggc tcagagttgc cggcaagaca tcgacgaagt gatcaaggcc ctgttcatcc
     2941 ttccaccgct gaacgggagc cttagcattc atccgggtgc tgtcgaagat ttcccacaaa
     3001 ctcgtcagcg ttgcttgatt cggcttcaaa ccctccatgg gccgtggaat aaggacctct
     3061 tcggttgcgg gagaagggcc tgagggacca ggtacgggat gaatttcagc ttggggtcgc
     3121 ccgggcaagg tcggctgaac tgtgaccttc ttcggcgatg cggatcgagg aaccggcgtc
     3181 acagtcgtcg ccgacttctt ttgtgtcgaa ggaggcggca tatcggttat atggagattc
     3241 ccttgatcat cagtccattt atacagttcc gcggacccga taaccggcca gaacagaaga
     3301 ccgctcatga ggcccagtgt caaaactttc atattgtgag atgccctcag tatccaaatg
     3361 atgttgtagt tgccaaaagt atagcaggca gtcccattta tgcctgggcg tacccgggca
     3421 cccgcgcatg gccggcactc ggtcggaagc agaaaagaag tccgggattg gagtgggagt
     3481 tggatcggac gctagggtgg gaggagcacg cacctaaccg aggacgatca ggtacggcgg
     3541 agatcttggg aggactttag gcttcccagt acaagctggg cctgcacacc atcctcgcgc
     3601 tcgatcccct gttgaaccat attccctcgg ggcgttaact cctcccacct gcgtacgata
     3661 ggcctcaact acgaatcagt caagaaaact aatgtgtcgt tccttacccg gttcacgaaa
     3721 gtcgtgggta cattcctctt gacgtttgaa atcgtaacgt gcagggtgct ctccctctac
     3781 aattcttgaa aggttggcaa tggctcaggc actcacgagt gaggacgtac aacaggcatg
     3841 gtcggcactc acagaagata ctcgcatagc cgtgctgctc acgatgcgaa acggacgacc
     3901 atttggatcg cacgtgccct atgtgtttgg atcggactgg accaaagcgt atctgcacct
     3961 cagccgactg gcgcttcaca cgcagcatct gctggctgat ccccatgtgt cgctgttcat
     4021 cgctgagacg gacggaccag agaaaaatcc tctcgcgcta agaagaataa atcttatggg
     4081 tcaagcggca ccgttgtcac ctgatacacc gtcgtatggc gagctcaagg agcgatacct
     4141 ctcgcggttt cctcaagccg agatgatgtt cggctttggt gattttgctc tctgggaatt
     4201 gcggcttgaa gacgcccact ttgtgctcgg cttcggtcaa gccttcgtct cgaccgtcgc
     4261 cactccatcc agatgggtgc atcaaaaagt ggaacgaccc actgcgagga aggactgatc
     4321 gaccttcgaa gagcagcgct cgcgaatata cacggccggc tcaacttacc gtcaaataaa
     4381 ttacaaaacc ccaacagata tgtgactcgc cgcctgcttc agagag
//
LOCUS       DATMTI010000838         3913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_73232, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000838 DATMTI010000000
VERSION     DATMTI010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73232"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VN039_13190"
     CDS             complement(<1..358)
                     /locus_tag="VN039_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HWV46953.1"
                     /translation="MKHLRQLEDQSVYILREAYKHFDNLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSYKIPAMIEYRDRLAREWRLNLVVGQNKEALAAGMNHTMGRVV
                     CCTALKTNGLKQLLEHK"
     gene            complement(383..1423)
                     /gene="trpD"
                     /locus_tag="VN039_13195"
     CDS             complement(383..1423)
                     /gene="trpD"
                     /locus_tag="VN039_13195"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWV46954.1"
                     /translation="MPIQDLLAKIAKGPKASKDLTWDECKQAMKALIEGEATPAQVGA
                     FLIAMRFKTESVTELAALTAAARQYVLPLTVSRDLALVDVPTYAGKQDTFHATIAASI
                     VAVAAGAAVLMHGYDGIPGRPGSAGVLRAMGIPVDADPKLVSEDVNRKGFAYLDIGLY
                     HPPLYRFLEMRQELGVRNVFHPIARLLNPARAAVQVVGLTHPPHFEKTAEVLRILGCR
                     RALVIRGVEGDPELSASMATRVLELRDERITPLGVAPKDFGLAFVPSREMAGFPPDQR
                     DREADLIRRILRNQVQGGPRDWVLMNAAMLLYAAGKGMSLSACFPAVRAALDGGAAGR
                     KLDELVRQPVAV"
     gene            complement(1425..2141)
                     /locus_tag="VN039_13200"
     CDS             complement(1425..2141)
                     /locus_tag="VN039_13200"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWV46955.1"
                     /translation="MTVNGRSELVAELKTWSASFETKQPQDILTAAIERYGPKIVVAC
                     SFGAEDVVLVDMVHRINASVPLFYLDTDFLFPETYATRDRVIERYDLQPTQVIQVKSL
                     LTPEVQAESYGDALWARDPDQCCRLRKVEPLTRVLRGYDAWITGIRRDQAPSRANVGL
                     IEWDQKFELIKINPLARWTSTDVWAYIKVYEVPYNPLHDQNYPSIGCTYCTAPVASGD
                     DPRAGRWKNFAKTECGLHKS"
     gene            complement(2158..>3913)
                     /locus_tag="VN039_13205"
     CDS             complement(2158..>3913)
                     /locus_tag="VN039_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWV46956.1"
                     /translation="VIKVFDTLGNRKNRNKARMKFVIDKLGFDEFKRRWEAAYVSMGH
                     ALPKNGTLTLLQHQDEPPRLIMPTRNGVANGNGNGNGTHLIGYETPFEMWKRTNVVKQ
                     KQDGYVTAAIKLFMGDITAEQMLFVADLAERYSNGNLRTTINQNLVIRWVPADQIPHF
                     YEDLASHGLADPGAELVEDIIACPGTDTCGLGITSSKGLARALAEVFPAGRVLEDLAG
                     VDVKISGCHNSCAQHHISTIGLHGVGKRLGDHVAPHYELHLGGSVNGTAKIGQMTVKL
                     PAKAVPAAISHLIDVYRRDRQANENLPKFIARVGKSKLKDELIPYTIVPSFQEDPTFY
                     YDWEGEEEFILEDLGPGECAGGALEMIENGILEADQELYQAKLLVEKHQYSVSVNKSY
                     RAVLAAAKAMLVTEGLEPSTDSETFIEFDSRIAQKDVVPSVYRDLSKKVGDLGPKETS
                     SESAREKMAFAKGFVDACRAATDQMGKDLKLSTMKEEKPSVAPASVETKPVAPAPTGA
                     PVYDLRGVACPMNYVKTKLKLEMMDAGEQLEVWLDAGEPIKNVPMSLKNDGHKVLIQE
                     ALEPEASHYRILVEKVEG"
BASE COUNT          840 a         1162 c          988 g          923 t
ORIGIN      
        1 ctttgtgctc caacagctgc ttgaggccgt tcgttttcag cgctgtacaa cagaccactc
       61 gccccatcgt gtggttcatc cctgccgcta aggcttcctt gttttgacca acaaccagat
      121 tcaaccgcca ttcgcgagcg agacgatcac ggtactcgat catcgctgga attttgtagc
      181 tggtatcgac atggagcagc ggaaacggca catgcccgaa gaaggccttg cgtgcaagcc
      241 aaagcaacac ggtcgaatcc ttccccatcg accaaagcat ggcaagattg tcgaaatgct
      301 tataggcttc tcgaaggatg taaacgcttt gatcttcaag ttgccgcagg tgtttcatat
      361 ccggaatcct tccactttcg tcctacactg cgaccggctg tcgcaccaat tcatcgagct
      421 tgcgccctgc ggctcctccg tcgagcgccg cgcgtacagc cgggaagcag gccgacaggg
      481 acatgccttt gcccgccgca tagagcaaca tggctgcatt catcaacacc caatctcgcg
      541 gcccaccctg aacctggttc cgaagaattc gccggatcag atcggcttcc ctgtcccgct
      601 gatcgggagg aaacccggcc atctctcttg atgggacaaa tgccagcccg aaatctttcg
      661 gagccactcc cagcggcgtg atgcgctcat cacgcagttc cagaactctc gtcgccatcg
      721 aggcggataa ctccggatca ccttcaactc cacggatcac caatgctcgt cggcatccga
      781 ggatccgcaa gacctcggct gtcttttcaa aatgcggcgg atgcgtcaac cccacgactt
      841 gcaccgccgc acgagccggg ttcagaagcc tggcgatcgg atggaacaca ttcctgacgc
      901 cgagctcttg acgcatctcc agaaatcgat agaggggtgg atggtacagt ccgatatcca
      961 ggtaggcaaa acctttcctg ttcacgtctt cggaaaccag tttgggatcg gcatcaaccg
     1021 gaatgcccat ggctctcagc actccggcac tgcccggtcg gccaggaatg ccgtcatagc
     1081 cgtgcatcaa gactgcagct ccggctgcaa ctgcaacaat agacgcggca atagtcgcat
     1141 gaaacgtgtc ctgcttcccg gcataagtcg ggacatccac cagagccaag tcacgcgaga
     1201 cagtgagtgg aagcacatat tgccgagccg cagccgtcag cgccgccaat tcggtaaccg
     1261 attccgtctt gaatcgcatg gcaatgagaa aagcccccac ttgcgccggc gtggcctcac
     1321 cttcaatcaa tgccttcatc gcctgtttac actcatccca tgtaagatcc ttggaagctt
     1381 tcggaccctt tgcaatcttg gcgaggagat cctgaatcgg catgttacga cttgtgtagc
     1441 ccgcattcgg tcttggcaaa attcttccag cgaccggccc gcggatcatc gcctgatgcc
     1501 actggggcgg tgcagtaggt gcagcctata ctgggataat tctgatcgtg cagggggtta
     1561 tacggaactt cgtagacctt gatgtaggcc cacacgtcgg tcgacgtcca tcgggccagg
     1621 ggattaatct tgatcaattc aaatttctgg tcccactcga tcaacccgac attcgcacga
     1681 gacggggctt gatcacgcct gattccggta atccatgcgt cgtatccgcg cagaacgcgg
     1741 gtcagcggtt cgactttccg aagccggcag cactggtccg gatcgcgcgc ccacaatgca
     1801 tcgccatagg attctgcttg gacctctggt gtgagcaatg acttcacttg gatgacctgc
     1861 gtcggctgaa gatcgtatcg ttcaatcact cgatcccgcg tggcataggt ttcaggaaac
     1921 aaaaaatcgg tatccagata gaacaacggc actgaggcat tgatccgatg caccatatcg
     1981 acgaggacca cgtcttccgc tccgaagctg caagccacaa caatctttgg gccgtatcgc
     2041 tcgatcgcag ccgtcaatat atcttgtggc tgcttcgtct cgaacgaagc gctccaggtt
     2101 ttgagttctg cgaccagttc ggaacggcca ttcacggtca tggcaaatca gatcctttta
     2161 tccttcgacc ttttcgacca gaatccgata atgcgaggct tccggttcca acgcttcctg
     2221 tatcagcacc ttgtgtccat cgttcttaag gctcattggc acattcttga tcggttcacc
     2281 cgcgtcgagc cacacttcca actgttcacc tgcatccatc atttccaatt tcaacttcgt
     2341 ctttacatag ttcattgggc atgccacgcc ccgcaagtca taaacaggcg cacccgtcgg
     2401 agcaggagca acgggtttgg tttcaactga tgcaggcgcg actgaaggct tttcttcttt
     2461 catagtcgac agcttcagat ccttacccat ctgatccgtc gccgcacggc aggcatcaac
     2521 aaagcccttg gcaaatgcca tcttctccct ggctgattcg gacgacgtct ctttcggccc
     2581 cagatcgccg accttcttgc tgaggtctcg atagacggac ggcacaacat ctttctgtgc
     2641 aatcctgctg tcgaactcga taaacgtttc ggagtccgtc gagggttcca gtccttctgt
     2701 caccagcatt gccttggcgg cggccagtac ggctcgatag gatttattca ccgacacgga
     2761 atactgatgc ttctcaacga gcagcttcgc ttggtagagc tcttgatcgg cctccagaat
     2821 gccgttttca atcatctcga gcgcaccacc ggcacattcg ccggggccga gatcctcaag
     2881 gataaactct tcttctccct cccaatcata atagaacgtg ggatcctctt gaaaagacgg
     2941 cacgatggta tacgggatca attcgtcttt cagtttgctc ttcccgacgc gtgcgatgaa
     3001 tttcggcaaa ttctcgttcg cttggcggtc gcgccggtac acgtcgatca aatgagagat
     3061 cgccgccggt acggcttttg ccggcaactt cacggtcatc tggccgatct ttgccgtgcc
     3121 gttcaccgaa ccaccgagat gcaattcata gtgcggcgcg acgtggtcgc cgaggcgttt
     3181 tccgactcca tgcaacccga tcgttgaaat gtgatgctgt gcgcaggagt tatggcatcc
     3241 gctgattttg acatcgactc ctgccagatc ttccagcacg cgaccggctg ggaacacctc
     3301 ggccagagcc cgcgcaaggc ctttggatga tgtgatccca agaccgcatg tatctgtgcc
     3361 ggggcaggca ataatgtctt cgaccagttc ggcaccggga tctgccaatc cgtgagatgc
     3421 caggtcctca taaaagtgcg ggatctgatc ggcaggaacc caccggatga ctaggttttg
     3481 attgatcgtg gtgcgaagat taccgttgga atagcgctca gccaggtcag ccacgaacag
     3541 catctgttca gctgtaatat cccccatgaa cagcttgatg gcggccgtga catacccatc
     3601 ttgcttctgc ttcacaacgt tggtccgttt ccacatctcg aacggggttt cgtagccgat
     3661 caggtgggtc ccgttaccat tcccgtttcc atttgccaca ccgtttcttg tcggcatgat
     3721 gagccgaggg ggttcatcct gatgctgaag caaggtcaag gttccgttct tgggaagtgc
     3781 gtgtcccatg gagacgtagg ctgcttccca gcgccgtttg aattcgtcaa atcccagttt
     3841 gtcgatcacg aacttcatcc gggccttgtt tcgatttttt cgattgccga gggtatcgaa
     3901 gactttaata acg
//
LOCUS       DATMTI010000839         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_115789, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000839 DATMTI010000000
VERSION     DATMTI010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115789"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..659
                     /locus_tag="VN039_13210"
     CDS             348..659
                     /locus_tag="VN039_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46957.1"
                     /translation="MSDAVLSTENSSGHRDTAEVHVTHVNDVESAKFEVSLHATLQSV
                     WDKAYLELKVAKQPNDVFQTAGNKPRSLMNYLAMTLAQAKEQGVITNFHFEIVSETGG
                     A"
     gene            659..1183
                     /locus_tag="VN039_13215"
     CDS             659..1183
                     /locus_tag="VN039_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46958.1"
                     /translation="MVAAAGQCPVVNTQAGLQALRQDVPRVLKTLGASSLEELGWIQI
                     DALTMLIPLSASINGITDHYLLKLGFHCYPDWPPSAQFVNPETKQYAWPADQHCVPKL
                     ESPECRTHPNYTSPRCLGIQLICCSATLEFYDVLHDVQPHHVWNGKSTFFTTLKAIQR
                     AMQSHYMGRFPRHE"
     gene            1176..1709
                     /locus_tag="VN039_13220"
     CDS             1176..1709
                     /locus_tag="VN039_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46959.1"
                     /translation="MNDEDLELRPQPRQPYQLGASCVVVPILALEGTLGLLRRAGRLE
                     SAVFWYGTKDAAGNATVSYVVAPQQLSRPRNYHVPAASVGDMVRRLKPGWKPIAQIHS
                     HPGDAVEHSNYDDRMAISTRALSLVVPRYGHWSGTFPERIGVHEWQNNYWYLLTMAHA
                     RSRIRLVNGEVVVEDLR"
     gene            1706..>3060
                     /locus_tag="VN039_13225"
     CDS             1706..>3060
                     /locus_tag="VN039_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HWV46960.1"
                     /translation="MKGQAKISSRHLLSVEGLADASHARKLRIAIYVASEWSCTLAGQ
                     HLLVCLINLMCRQTDLVDHIDVVCDEHPLKVTCPGEFEQDMLLRSLRVLVEWAVGGEV
                     TIGFPSKPGQVDMTIAIGCPDQFPVDLRSDTLFAVASGWLAWVGVGERAPRGIIPAKE
                     DPMGPFLAASILAGEVFKQARRTTRGAPVQAAGYSLWTRAPSEAWLNLEDGPTLRGQM
                     LPPLHVIGAGAVGHALIYTITAAALGASYLVLVDDDVYDETNLNRCLLAGKRDVSKAK
                     VHTIERYVKRSGGDVAAFPCALQEYLVGPKTALRQDVADAVRNFQFDLVASCVDKNTS
                     RQSIQGLWPALIVGGSTLGLKARSDIYSVKESTACLACHNPPEPDGEKIRALQARLRA
                     MPAQERRHFLLEHALPADAIEAYLASPECGKVGEAEFKAFALQGAPQFSVGFVSMMAG
                     IL"
BASE COUNT          671 a          849 c          867 g          673 t
ORIGIN      
        1 gtcaagctcc aaagcgaaaa ctttgacatg tgtttaaacg gaatttatgg aattttcggc
       61 gcaaggactc actccgcgat cgttgacgga cgaggctttg agaggaagta atcgccttca
      121 gaggtgggag cggcgtccca acggactcac ggcaaaagag gagtgaaatc catgacccaa
      181 aaatagaaac ggtcgagtac gaaaccgtac tcgaccgtgg gtagaacgag cgttgaggct
      241 actcgtccgg tttggcgacc acgattctcc gcacgctcct tcgcacagtc aagcaccaat
      301 atttgctggc actgcgccgg caggtccttt gctatcggag aatcgttatg tctgatgcag
      361 ttctttccac cgagaattcg tctggtcatc gcgacaccgc tgaagtgcat gttacgcacg
      421 tcaacgacgt cgaatcggcc aagtttgaag tgtcactaca cgcaaccctg cagtcagtgt
      481 gggataaggc ttacctagaa ctgaaggttg ccaagcaacc caatgacgtc ttccagacgg
      541 cgggcaataa gcctaggtca ttgatgaact accttgcgat gactttggcg caagctaagg
      601 aacaaggtgt catcacgaac ttccacttcg agattgtctc ggaaactggt ggagcgtgat
      661 ggtggctgcc gcaggacaat gtcccgtcgt gaatacgcaa gcgggcctgc aagcccttcg
      721 tcaggatgtg ccaagggttc ttaagaccct tggcgcatcc tcgcttgagg aactgggctg
      781 gatccagatc gatgcgctaa cgatgttgat ccccctttct gcatcgatca acggcatcac
      841 agaccattac ctcttgaagc tcgggttcca ctgctatccg gattggccgc cgagcgcgca
      901 attcgtcaac ccggaaacga aacaatacgc ttggcccgcg gatcagcact gcgtgcccaa
      961 gcttgagagt cctgagtgcc gcacgcaccc caactacacg agccctaggt gcctgggaat
     1021 tcaattgatt tgctgctcag cgacgctcga gttctacgac gtcttacacg acgttcagcc
     1081 tcaccacgtg tggaacggaa aaagcacgtt ctttacgaca ctaaaggcaa ttcaacgcgc
     1141 catgcagagc cattacatgg ggaggttccc tcgccatgaa tgacgaagat ttggagctgc
     1201 ggccgcaacc gaggcaacca taccaactgg gcgctagctg cgtagtggtc ccgattctcg
     1261 ctcttgaggg cacgctcggg cttctccggc gggctggaag actcgaatct gcagtgttct
     1321 ggtatggcac taaggatgcc gctggcaacg ccactgtctc ctatgttgtc gcaccccagc
     1381 aactctcacg gccgaggaat taccacgttc cggctgcttc ggtgggtgac atggtaaggc
     1441 gattgaagcc cggctggaaa ccgattgcgc aaatacatag ccaccccggc gacgccgtgg
     1501 aacattccaa ttacgacgac cggatggcca tctcgaccag ggcgttatct ctcgtagtac
     1561 cccggtacgg gcactggtcg ggcacgtttc cagaacgaat tggtgtccat gagtggcaaa
     1621 acaattactg gtacctgctg accatggccc acgctcggtc acgtatacga ttggtgaatg
     1681 gcgaggtggt ggtggaggac cttcgatgaa gggacaggca aagatttcta gtcgccatct
     1741 tctctctgtg gaagggctgg ctgacgcgag tcatgcacgc aaactccgca tagcaatata
     1801 cgtcgcctcc gaatggagct gcactctagc agggcagcat ctactggtct gcctgatcaa
     1861 tctcatgtgt aggcagacgg atttagtcga tcacatcgat gtggtgtgtg atgagcaccc
     1921 cttgaaggtg acatgtccgg gggaattcga gcaggacatg ctgctccgat cactgcgtgt
     1981 cttggtggag tgggctgtcg gcggcgaagt gactatcggc tttccttcaa agccaggtca
     2041 ggtcgacatg acgatcgcca tcgggtgccc agatcaattt cccgtcgacc ttcggtcgga
     2101 caccctcttc gcggtcgcct ctggctggtt ggcttgggtc ggtgtcgggg aacgcgcacc
     2161 tcgcggaatt attccggcca aagaagaccc gatgggtcca tttctagcag caagcattct
     2221 ggcaggtgag gtgttcaagc aggctaggcg aacgacccgg ggtgcgccag ttcaagccgc
     2281 cgggtactcg ctctggacac gtgcgccgtc tgaagcctgg ctcaatctcg aagatggccc
     2341 tactttgcgg gggcagatgc ttccaccctt gcatgtcatt ggcgctggcg ctgtaggcca
     2401 tgcactcatt tacaccataa cagcggctgc cctcggtgcc tcctacttgg tgcttgtgga
     2461 cgatgacgtg tacgacgaga ccaaccttaa tcgctgtttg ttggccggta agcgcgacgt
     2521 cagtaaggcc aaagtgcaca ccatcgaacg ctatgtgaag cggtcgggtg gggatgtcgc
     2581 tgcgtttccc tgcgctttac aagaatatct tgttgggccc aagacggctc tgcgccaaga
     2641 cgtggccgac gcagtgcgaa actttcaatt tgaccttgtt gcctcttgtg tggacaagaa
     2701 cacgtcgaga caaagtatcc agggtctttg gccagcctta atcgttggtg ggagtactct
     2761 cggcctcaag gcgagatccg atatctatag cgttaaggaa agcactgctt gccttgcctg
     2821 ccacaatcct cccgagccag atggcgaaaa gatccgggcc cttcaggcga ggcttcgagc
     2881 catgcctgca caggagcgac gccacttcct tctcgaacat gctctcccgg cggatgccat
     2941 cgaggcctat ctcgcttcac ctgagtgcgg aaaagttggc gaggccgagt tcaaagcatt
     3001 cgcccttcaa ggagcgccac agttctccgt gggctttgta tccatgatgg ccggcatcct
//
LOCUS       DATMTI010000840         4690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_541164, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000840 DATMTI010000000
VERSION     DATMTI010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541164"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VN039_13230"
     CDS             complement(<1..514)
                     /locus_tag="VN039_13230"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HWV46961.1"
                     /translation="MGRRVPYDRYVVGIVAIMGLLGGNVYAGESNDKKTDDPAIKEVL
                     DCRYRQASELSEGKATSEVFPSDDLFRPLLADPKQPQFFALWQSTTSNVAQSAKSNGL
                     VGFGENFGFYTKRQGCNGWQISLLTGVFAQFDLDTAHTAFTNVDFNVGIPLTWRQGNW
                     SGRLRFYHQSS"
     gene            complement(686..3118)
                     /locus_tag="VN039_13235"
     CDS             complement(686..3118)
                     /locus_tag="VN039_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWV46962.1"
                     /translation="MMKGNTPPLNAGLLKQMDAYWRAANYLSVGQIYLLDNPLLRKPL
                     TLEHIKPRLLGHWGTTPGLNFIYVHLNRVIKRDDLNMIFICGPGHGGPAVVANVYLEG
                     TYSEVYPDVSQDEEGMKRLFRQFSFPGGIPSHVAPETPGSIHEGGELGYALSHAYGAA
                     FDNPDLIVACVIGDGEAETGPLAASWHSNKFLNPVTDGAVLPILHLNGYKIANPTILA
                     RISHDELHSLLVGYGYEPYYVEGSEPEPMHHAMAGTLDAVLARIKAIQQKARQEAAEG
                     KGLPARPRWPVIVLRSPKGWTGPKEVDGKKTEDYWRSHQVPMAEMASKPAHIGLLESW
                     MKSYRPDELFDANGTLMPELAALAPTGQHRMGANLHTNGGLLLRDLHLPDFRDYAVDV
                     SMPGAVVDEATKVLGTFLRDTVTKNMRNRNFRIMAPDELTSNRLGAVLDVSDRVWMAE
                     REPNDDHLAPDGRSMEILSEHTCQGWLEGYLLTGRHGLFTTYEAFVHIVDSMFNQHAK
                     WLKTCHEIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVVNKKADVIRVYLPP
                     DANCLLSVMDHCLRSRDYVNVIVAGKQPALQYLDMEAAVKHCTAGIGIWEWASNDKGG
                     EPDVVMGCAGDVPTLETLAAVQLLREYVPSLKIRVVNVVDLMKLQDKNEHPHGLPDKH
                     FDTFFTTDKPIIFAYHGYPWLIHRLTYRRTNHKNLHVRGYKEEGTTTTPFDMVFLNDM
                     DRYHLASDVIERVGLGSAAAYAKQAIRDKRIEHKEYIRKHGEDMPEIRQWKWDASKSF
                     MGRTRSATDAPG"
     gene            complement(3128..4339)
                     /locus_tag="VN039_13240"
     CDS             complement(3128..4339)
                     /locus_tag="VN039_13240"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HWV46963.1"
                     /translation="MNRQESSPFILTINGGSSSIKFALFRAVDLSVPLTRGMVDRIGL
                     PNSEMLVTDTATQQSSRRAVHAPDHRTCVGFITEWLEQQVGIGRLCAVGHRVVHGGRT
                     YSRPERVTPELLEELRRLCPYSPEHLPAEIILIESFRRHDPTLTQIACFDTAFHRDMP
                     RVARLLPIPRRYEKLGIQRYGFHGLSYAFLMRELTRMGALREAEGRVILAHLGNGASM
                     AAVKNGQALDTTMGFTPASGLPMSRRSGDLDPGLVSYLAHTEGMTVERFHGMVNTESG
                     LLGVSEISSDMRDLLKQERSDPRAAEAVGLFCYQARKWIGALAAALGGVDSLVFSAGI
                     GEHAPAIRARICAGLEFLGIVINEPANEANQPVISSEASRVRVRVIPTDEEREIAESV
                     VRLLGKENMDH"
     gene            complement(4367..>4690)
                     /gene="rnk"
                     /locus_tag="VN039_13245"
     CDS             complement(4367..>4690)
                     /gene="rnk"
                     /locus_tag="VN039_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HWV46964.1"
                     /translation="GYLESLQNELDRAHIIEPSTIPDNVVTMNSQVRLKDMETGEEKS
                     YTLVFPSDADIEKNRISILAPIGTAILGYRAGDTVDWLVPAGTRKVRIEEILYQPEAA
                     GRYDL"
BASE COUNT          967 a         1418 c         1278 g         1027 t
ORIGIN      
        1 gactgctctg gtgataaaaa cgcaggcgcc cggaccagtt tccctgacgc caggtcagtg
       61 ggatgccgac attgaaatcg acgttggtga acgcggtatg cgccgtatcc aagtcaaact
      121 gggcgaatac cccggtcagg aggctgatct gccatccgtt acagccctgg cgcttggtgt
      181 aaaacccaaa attttctcca aagccgacca atccgttgct cttagcgctc tgcgcaacgt
      241 ttgacgtggt ggattgccag agggcaaaga attgcggctg tttcgggtcg gccaagagag
      301 gacgaaagag atcgtccgat ggaaacacct cgctcgtcgc ttttccttcg gagagctcag
      361 acgcttgccg ataccgacaa tccagcacct cttttatcgc cggatcatcc gtcttcttat
      421 cgtttgattc acctgcgtag acgtttcctc ccagcaggcc catgatggcc actatcccga
      481 cgacatatcg atcatacgga actcgccgac ccatgccgtt cctcccatcc gacgttggtg
      541 tgtgatgcaa acccagcgcg tttcaaatag catatttcca gtgggaaatt cacatggttt
      601 cctcgcgcat ggcagccggt cggtggagac ttctttcatc atgtcgaaat acgaaagatg
      661 tgatgggtat gcgaagaacg gatgatcaac cgggagcatc cgtcgccgac cgtgtccttc
      721 ccatgaaact cttgctcgca tcccatttcc attggcggat ctccggcatg tcttctccgt
      781 gcttcctgat gtattccttg tgctcgatcc gtttgtcgcg gatggcctgt ttggcatagg
      841 ccgccgccga tcccaagccg acgcgttcga tcacgtcgga agccaggtga taccggtcca
      901 tgtcgttcag gaagaccatg tcgaacggcg tggtggtcgt gccctcttcc ttgtagccac
      961 gcacgtgcag attcttgtga ttcgtccgtc ggtaggtgag ccgatgaatc aaccagggat
     1021 agccgtgata cgcgaagatg atcggcttgt cggtcgtgaa gaatgtatcg aaatgcttat
     1081 ccggcaaccc gtgcggatgc tcgttcttgt cctgcaactt catcagatcg accacgttga
     1141 cgacccggat cttgagcgac ggcacgtatt cgcgcaagag ctgcacggcc gcgagcgtct
     1201 ccagcgtggg cacgtcgccg gcgcatccca tcaccacatc cggctccccg cccttgtcgt
     1261 tgctcgccca ttcccaaatg ccgatgccgg ccgtgcaatg tttcaccgcc gcctccatat
     1321 cgagatactg cagagccggc tgcttgccgg cgacgatcac attcacgtaa tcgcggctgc
     1381 gcaggcagtg gtccatgacc gacaacagac aattggcatc cggcggcaga tagacccgga
     1441 tgacgtcggc cttcttgttc acgacatggt cgatgaaccc cggatcttga tgggagaatc
     1501 cgttgtggtc ctgccgccag acgtgggacg tcagcaaata gttcagtgac gcgatgggcc
     1561 gccgccacgg aatctcatga caggtcttga gccacttggc atgttggttg aacattgaat
     1621 cgacgatatg gacgaaggct tcataggtcg taaacagtcc gtgccggccc gtgagtagat
     1681 acccttcgag ccagccctgg cacgtatgtt cactcaggat ttccatcgag cggccgtccg
     1741 gagcgagatg atcatcgttc ggctctcgtt ccgccatcca gactcggtcc gagacatcca
     1801 gcacggcgcc gagccggttc gaggtgagct cgtcgggagc catgatacgg aaattccggt
     1861 tgcgcatgtt cttggtaacc gtgtcgcgta aaaatgtacc cagtaccttg gtcgcctcgt
     1921 cgacgacggc tcccggcatc gacacgtcca cggcatagtc gcggaaatcg gggagatgaa
     1981 ggtcccgcag cagcagtccg ccgttggtat gaaggttggc ccccatccga tgctgtcctg
     2041 tcggcgcgag tgcggccagt tcgggcatca atgtgccgtt cgcatcgaac aactcatccg
     2101 gccgatagct tttcatccag gattccaaga gcccgatatg ggccggtttg gatgccatct
     2161 ccgccatcgg cacctgatgc gatctccaat agtcttcggt tttcttgccg tcgacctcct
     2221 tggggccggt ccatcctttg ggactgcgca agacgatcac gggccaccgt ggccgagcgg
     2281 ggaggccctt gccttcagcc gcttcctgtc gggccttctg ctgaattgcc ttgatacgcg
     2341 ccaacaccgc atccagcgtg cccgccatcg catggtgcat cggctccggc tctgagcctt
     2401 cgacatagta gggttcgtag ccgtatccga cgagcaggct gtgcagctcg tcatgactga
     2461 tccgtgccag gatggtcgga ttcgcaatct tgtagccgtt caagtgcaga atcggcagca
     2521 cggctccgtc ggtgacggga ttgaggaatt tgttcgaatg ccacgaggcc gccagcgggc
     2581 ctgtctccgc ctctccatcg ccgatgacac aggccacgat caaatcagga ttgtcgaagg
     2641 cggccccata ggcatgggac agcgcgtacc ccaattctcc gccttcgtgg atcgagcccg
     2701 gcgtctcggg cgcgacgtga ctcgggattc cgccggggaa agaaaactgc cgaaacaggc
     2761 gcttcatgcc ttcttcgtcc tgactcacgt ccggatacac ctcgctgtag gtaccttcaa
     2821 gatagacgtt cgccacgacc gcgggaccgc catggccggg cccgcagatg aagatcatat
     2881 tcaggtcgtc ccgtttgatg acccgattca gatggacata gatgaaattc agtcccggcg
     2941 tcgtgcccca atgacccaat aggcgcggct tgatgtgctc gagcgtcaag ggttttcgca
     3001 gcaacggatt gtcgagcaga tagatctgtc ccaccgaaag ataattggcg gcccgccaat
     3061 aggcatccat ctgcttcagt aagccggcat tcaatggtgg tgtgtttccc ttcatcataa
     3121 ttacctctca gtgatccata ttctccttgc caagtagccg aacaacggac tctgcaatct
     3181 cgcgttcttc atcagtgggg atcacgcgca ccctcactcg acttgcttcg gatgaaataa
     3241 ccggttgatt ggcctcattg gcgggttcat tgatgacaat gcccagaaat tccaacccgg
     3301 cgcagattcg cgctcgaatc gcgggagcat gctccccgat cccggcactg aagaccaagg
     3361 aatcaactcc tcccagcgcc gcggccagcg caccgatcca ttttcgagcc tgatagcaga
     3421 acaatccaac cgcctcggcc gcccgtggat cgctccgctc ttgcttgagt aagtcccgca
     3481 tatcggaact gatctcggag accccgagca agcccgattc ggtattgacc atcccgtgga
     3541 accgctccac ggtcatccct tccgtgtgag ccagatagga aacgagccct ggatcgaggt
     3601 cgcctgatcg acggctcatc ggaaggcctg aggcgggagt gaaacccatc gtcgtatcca
     3661 gcgcctgccc attcttgacc gccgccatgc tcgcgccatt tcccagatgg gctagaatga
     3721 cgcggccctc agcctcgcga agcgcaccca ttcttgtcag ttctcgcatg agaaacgcat
     3781 acgacaaccc gtgaaaccca tagcgctgaa tacccaactt ttcgtaccgc ctcggaatcg
     3841 gcagcaatcg cgcaacccgc ggcatgtccc ggtgaaatgc cgtatcgaaa caggctattt
     3901 gggtaagagt cggatcatgc cgacgaaacg attcgatcaa gataatttcc gccggcagat
     3961 gctccgggct gtaagggcat agtcggcgaa gctcctccag caactcgggc gtcactcgtt
     4021 cgggtctgct ataggtcctc ccgccatgga cgacacgatg cccgacggca cagagacgcc
     4081 caataccgac ctgctgttcc aaccattcgg ttataaaccc gacgcaggtc cgatggtccg
     4141 gtgcgtgaac ggcccgacga ctcgattgct gcgtggccgt atcggtgaca agcatttcgc
     4201 tgttcggcag acctatccga tcgaccatgc cgcgcgtcaa cggcacggag aggtccacgg
     4261 ctcgaaatag agcaaacttg atactggacg atcctccgtt gatcgtgaga atgaacgggc
     4321 tcgattcctg tcgattcatc ttgtcagcac aattcacatg cgactgttac aagtcataac
     4381 gtccggctgc ctcgggctga tagagaattt cttcgatccg taccttgcgt gtcccggccg
     4441 gtacaagcca gtccaccgtg tcacccgccc ggtagccgag gatggcggtg ccgatgggag
     4501 caagaatcga gatgcgattc ttctcaatat cggcatcgga cggaaatacg agggtgtagc
     4561 ttttctcctc gcccgtctcc atatccttga ggcgaacttg agaattcatc gtgaccacat
     4621 tatcaggaat tgtgctgggc tctatgatat gcgcccggtc caactcattc tgtaaactct
     4681 ccagatatcc
//
LOCUS       DATMTI010000841         6572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_498924, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000841 DATMTI010000000
VERSION     DATMTI010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498924"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="zwf"
                     /locus_tag="VN039_13250"
     CDS             complement(<1..1260)
                     /gene="zwf"
                     /locus_tag="VN039_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWV46965.1"
                     /translation="MTHTNRQRIDMSPAQEPLPLIEPCTLVIFGGSGDLARRRLIPAV
                     YNLLLDGLLPSNYVVLGLGRTPMSDEEFRSTVRDGVIKHSRQALIEDTWTAFSQHLFY
                     LAGGNDDAPTYARLKERVEELERTFQLPGNRIFYLSIPPSSFTAVCEGLSHSGLAGNP
                     AAAPPYTRIIVEKPVGRDLTSAQAINEVTGRVFDESQIFRIDHYLGKETVQNLMVVRF
                     ANSIFEPIWNYKYVDHVQITVSEAEGVGTRATYYEEAGALRDMVQNHLLQLLCLVAME
                     PPYSLDPNVVRNAKMEVLRCLRPITAKDVEKFTVRAQYTEGTAHGAPVPGYRREKGVK
                     PNSTVETYVAVKCFVENWRWSGVPFYLRTGKALPLRASEVAVQFKEIPQILFNAGGQT
                     PQAPNVLALKIQPEEGLTLRIVSRVPGT"
     gene            complement(1427..2320)
                     /gene="gnd"
                     /locus_tag="VN039_13255"
     CDS             complement(1427..2320)
                     /gene="gnd"
                     /locus_tag="VN039_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWV46966.1"
                     /translation="MELGFIGLGKMGMNMVTRLRRDQHRVVVYDRSNELIKQAEGQGC
                     VGSSSLPDLVGKLSVPRVVWVMVPSGAPTEETIQAVAALLQSGDIIVDGGNTRFHDDV
                     RRAAELKKKGIHYVDAGTSGGIWGLKVGYCLMVGGEDAAVKRLEPALKTLAPENGWAH
                     VGAVGAGHYVKMVHNGIEYSMMQGYAEGFELMSKSEYKLDLARVADLWMHGSVVRSWL
                     LELAASALKDDQKLEKLKGYVQDSGEGRWMIADAIEKDVPVPTLTTALFTRFRSRQEE
                     SFAEKMLAALRNAFGGHAVRR"
     gene            complement(2453..3238)
                     /locus_tag="VN039_13260"
     CDS             complement(2453..3238)
                     /locus_tag="VN039_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46967.1"
                     /translation="MDTHTTTSTPTREELNAELLEVPEPPEQPESPPPPGFEDNTEVA
                     LRHIKGRRGGDLVGVILVGSGARRALTLHSDIDLIALVKGEADGHEILRVGDRLVDIR
                     YHGYHAVEEDLAYSLRLPSLLRKGRILYDHDGICAKLLEQVHHRFRQGPPPASINEQI
                     RMKAECYHLLEKSQDLVDKPGTAHYLLTLFYEDCISAFFRLRGFWLVAPVDVHRFMFS
                     REPVLADLAGQFLTATTLADRLNFGRQFVDLLFKDVPNPARID"
     gene            complement(3404..4435)
                     /gene="ltaE"
                     /locus_tag="VN039_13265"
     CDS             complement(3404..4435)
                     /gene="ltaE"
                     /locus_tag="VN039_13265"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HWV46968.1"
                     /translation="MIDLRSDTVTKPTDDMRKAMARAEVGDDVYGEDPTVNRLQDMAA
                     AMLGKRFALFVPSGTMANQLAIRSHTQPGQEIIVESKSHVVRYEQGAAGALAGVQLHW
                     VTGERGIMTAEQVEAAIRPNDMHSIATALICIENTHNAGGGTIYPLSTIEKIRALAVR
                     HGIPMHLDGARLFNAVAATTLPPTVYAQHFETVSLCLSKGLGAPVGSLLISNDQRLMD
                     RARRFRRMYGGAMRQAGILAAAGIYALERHVARLKTDHDHAKKLARLLQQIPSIEIAP
                     QHVETNIVMFDIADEQRCPVELVAALKEHGVLINAVGGQSYRAVTHLHITDKQIDEAV
                     AVFAKILTR"
     gene            4527..5846
                     /locus_tag="VN039_13270"
     CDS             4527..5846
                     /locus_tag="VN039_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV46969.1"
                     /translation="MGELGQTVLIRWADSLGAKREELVPLAWAFVYFFCLLCGYSILR
                     PVRDEVAIEGGLKHLPWMMTVTFLTMLGATPLFGWLSARCSRYRLLLTVYTFFITNLL
                     VFYALMTSHLYPEWVARGFFVWLSVFNLFIVSVFWSFMDDLFTPEQGARLFGVIAAGG
                     SSGALLGPLLTTGLTFVFPIQVLMVASSLFLGACIGCMYRLERWGRTQTLHHRPQQGE
                     PLRGSFLAGVRLTFSSPYLLGICGYLAILTMTATVLYFEQTRLVSEYLDRPEARTRLF
                     STLDFTTSLLTWLTQVFMTRRLIGRFGLVAALLSLPAISLVGFLGIALWPSLAVYVVF
                     SVLRRVGEYALSKPAREVLFTVVSREEKYKAKNFIDTAISRGGDASTAWLVTAVKAVG
                     ATPTHIAWTLVPLMGVWAWLATVLARQEKHRSASTLSSTTENSRSTA"
     gene            complement(5877..>6572)
                     /locus_tag="VN039_13275"
     CDS             complement(5877..>6572)
                     /locus_tag="VN039_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46970.1"
                     /translation="QSAEREREGQPPKVVQARPNNSSPQQCLINFDDENALEHIFGQA
                     RTTLAFRTSRIGFGPLQMCDPSKHSDCWAYRQRCARSDVNFDTIGHTHFHLNMETGVE
                     CYTLPDPGDGLGRGFGKLVDFKCTDVDWTKTPRVLSSHDQNQWIKIWVGNVDNHAPMY
                     FDMESISILPDTSIQLWFRKRDGTWWFWPELKAGRTWNIHNQVRDVSEVRIRGTKSGR
                     ASSYVIGSLVIRD"
BASE COUNT         1314 a         1829 c         1885 g         1544 t
ORIGIN      
        1 ggtacccggt acccgtgaga caatacggag cgtgagtcct tcctcaggct ggattttcaa
       61 cgctaagacg tttggagctt gaggcgtctg tccaccggca ttgaagagaa tctgcggaat
      121 ctctttgaat tggacggcga cttcgctggc acgaagcggc aacgctttcc ctgttcgcag
      181 gtagaagggg acgccggacc atcgccagtt ctccacaaaa catttcaccg ccacataggt
      241 ttcgacggtg gagttgggtt tcactccttt ttcacggcga taaccgggca ctggagcgcc
      301 atgtgcggtc ccctccgtgt attgggcccg cacggtgaac ttttccacat ccttggccgt
      361 gatgggccgc aagcagcgca gcacttccat cttggcgttt cgcacaacgt tcggatcgag
      421 tgagtatggt ggttccatgg cgacgaggca gagcaattgc agtaaatgat tctgaaccat
      481 atcacgcagc gcaccggctt cttcgtagta cgtcgccctg gtccccacac cctcggcttc
      541 gctcacggtg atttgaacgt ggtcgacgta tttgtagttc cagatcggct cgaagatact
      601 gttggcgaag cgcacgacca tgagattctg aaccgtctcc ttgccaagat agtgatcgat
      661 ccggaaaatc tgcgattcat cgaaaacgcg gcccgtgact tcattaatgg cctgcgcgga
      721 tgtcaggtca cgcccgaccg gtttttccac gatgatccgt gtgtaaggcg gagcagccgc
      781 agggtttccc gctagaccgg aatgggacaa gccctcacag accgcagtaa aggaactggg
      841 ggggatgctg agatagaaaa tccggttccc aggcaattga aacgtacgct caagctcctc
      901 cactcgctct ttcagccgag cgtaggttgg agcgtcgtcg tttccacctg ctaaataaaa
      961 caggtgttga gaaaaggcgg tccatgtatc ttcaattaac gcctgtcggg aatgtttgat
     1021 tacgccatcg cggacggtcg accggaattc ttcatcactc attggggtgc ggcccagacc
     1081 gagcacgacg tagttcgatg gcaacaaccc gtcgaggagg agattataga ccgcgggaat
     1141 cagacgccga cgagccaagt ctccggagcc gccgaaaatc accagcgtac aaggttcgat
     1201 gagtggcagc ggttcttggg ccggactcat gtcaatccgt tgtctattcg tgtgggtcat
     1261 cagggtctcc tatgcaggat gttgaaaaag tccgccagct gcgtgcttgc atcgcggatc
     1321 tctctgcggc cttgcccgag gagaagcgcg tctcggcgcg acggggttgg gcgggtaaga
     1381 tcgacggcct ttttgaacat cctgcagatc attctagtct ggttcgttat cgccggacgg
     1441 catggccacc gaaggcattc cgcagggccg ccagcatctt ttccgcaaac gattcttcct
     1501 gccgcgatcg aaaccgagtg aagagcgcgg tggtgagggt gggaaccggc acatccttct
     1561 cgatggcatc ggcaatcatc caccggcctt cgccggaatc ttgcacgtac cctttcagtt
     1621 tttccagctt ctgatcgtct ttgagcgcgc tcgcggccag ttccaaaagc caggatcgga
     1681 cgacacttcc atgcatccac aagtcggcca cgcgtgcaag gtcgagcttg tactcactct
     1741 tcgacatcaa ttcaaatcct tcggcatagc cttgcatcat gctgtactcg atgccgttgt
     1801 gcaccatctt cacatagtgc ccggcaccca ctgcgccgac atgggcccag ccgttttcag
     1861 gcgccagagt cttgagcgcc ggttcgagcc gtttgacggc tgcgtcttct ccaccgacca
     1921 tgagacaata gccgactttc agtccccaga ttcctccgct ggttcccgca tcgacatagt
     1981 ggatgccttt tttcttcagc tcggcagcac gccgcacatc gtcgtgaaat ctcgtgtttc
     2041 cgccgtccac gatgatgtcg ccggactgta gcaacgcagc cacggcctgg atggtttctt
     2101 cagtaggagc tcccgaagga accatcaccc agacgacacg cgggacactc agttttccga
     2161 cgagatcagg gagggaagaa gaaccgacac agccctgtcc ctcagcctgt ttgatcaact
     2221 cgttggatcg atcatacacc accacgcgat gctgatcgcg ccggaggcgt gtcaccatgt
     2281 tcattcccat cttaccgagg ccgatgaatc ccagttccat aagagagctc cttggtttgt
     2341 cgcacagccg atgcggtgac tgtcgatcga tctacatgct agtgcggcga ctcctctcac
     2401 gttctgtgcg aaatatgttt gttacgcaga ggcagccggt tgaatactcc ggtcagtcga
     2461 tacgagcagg attcgggaca tccttaaaaa ggagatcgac aaattgccgg ccaaaattga
     2521 gacgatcggc gagggtggtg gccgtcagga attgtccagc caggtcggcg agcaccggtt
     2581 cgcgcgaaaa catgaaccga tgaacgtcta ccggggcgac caaccaaaac cctctcaaac
     2641 gaaagaacgc cgagatgcaa tcttcgtaaa acaacgttaa tagatagtgg gccgttcccg
     2701 gcttgtccac aagatcctgc gatttttcca gcagatgata acactccgcc ttcatgcgaa
     2761 tctgttcgtt gatcgaagcc ggtggcggac cttggcgaaa acggtggtgg acctgttcga
     2821 gcaattttgc acagatgccg tcgtgatcgt aaagaattct gccttttcga agcagcgaag
     2881 gaagtcgaag cgagtacgca agatcttctt cgacagcgtg gtatccatgg tagcgaatat
     2941 cgaccagacg gtcgccgaca cggagaattt cgtgaccgtc ggcctctccc ttgaccaggg
     3001 cgatcaagtc gatatcgctg tggagagtca gggccctccg agctcccgat ccgacaagta
     3061 tgacaccgac gagatcaccg ccccttctgc ctttgatgtg ccgtaatgcg acttctgtgt
     3121 tgtcctcaaa tcccggggga ggcggtgatt ccggttgctc cggaggttcc gggacttcga
     3181 gcagctcggc gttcagttct tcacgagtgg gagtgctggt tgtggtgtgc gtgtccataa
     3241 cgtgcttaaa cttgagggga taagttatgt tcggaaacca tataatcgat cagcaaggac
     3301 ttgcaagtca ttctcgcaat gtgttgcatg gaacgcaacc ggcaaatgac ggtgccgtta
     3361 aggcatttta tgggtggagg aatatggtgt caacgttgaa tgatcaacgt gtgaggatct
     3421 tggcaaagac ggccacggct tcgtcgatct gcttgtccgt gatatgcaga tgggtgactg
     3481 cccgatagct ctggccaccg acggcgttga tgagcacacc gtgctctttg agcgcggcca
     3541 ccagttcgac cggacagcgc tgctcgtcag cgatgtcgaa cataacgata ttcgtttcta
     3601 cgtgttgcgg cgcaatctcg atcgagggaa tttgctgcag caggcgagcc agttttttcg
     3661 catggtcatg gtcggtcttg agccgagcga catgccgttc caaggcatag atgccggctg
     3721 cggccaggat gcccgcttga cgcatcgcgc ctccatacat gcggcggaag cggcgtgcgc
     3781 gatccatgag tcgctggtcg ttggagatca gcagcgatcc aacgggagct cccagccctt
     3841 tcgagagaca gagcgagacg gtttcaaagt gctgggcata taccgtcgga ggcaaggtgg
     3901 tggcggctac ggcattgaag agcctggccc cgtcgagatg catgggaatt ccatgtctca
     3961 cggcgagggc tctgattttt tcgatcgtgg agagcggata aatcgtgccg cctccggcat
     4021 tgtgcgtgtt ctcgatgcag atcaaggccg tcgcgatgct gtgcatatcg ttcggtctga
     4081 tggcggcttc cacctgctcg gcagtcatga tcccccgctc accggtcacc caatgaagtt
     4141 gcacacccgc cagcgccccg gccgctccct gttcatatcg aacgacatga ctcttgctct
     4201 cgacgataat ttcctgtccc ggttgagtgt gtgatcgaat cgccagttga ttggccatgg
     4261 tgccggaagg gacgaagagc gcaaaccgct tgccgagcat ggccgccgcc atatcttgga
     4321 ggcgattgac ggtcgggtct tcgccgtaga cgtcatcgcc gacctcggca cgcgccatgg
     4381 ctttccgcat gtcgtccgtc ggcttggtga cggtgtcgct acgaaggtcg atcatctgtg
     4441 ctgtctccta agaattccgt acggtccgag tattctaaca gatctgtcgc ttgcaggtga
     4501 aagcacacct gctacactgg acacgcatgg gcgagctggg tcaaacagtg ctcattcgat
     4561 gggctgattc cctcggcgcg aagcgggaag aactggtccc attggcttgg gccttcgtct
     4621 attttttctg tcttctgtgc ggctactcca ttctccgtcc ggtacgcgac gaagtggcca
     4681 tcgaaggagg attgaaacat cttccctgga tgatgaccgt gacattcctc accatgctcg
     4741 gtgccacgcc gctgttcgga tggctctcgg cacgatgctc gcgctaccgg ttgctgctga
     4801 ccgtctatac attcttcatc acgaatctgt tggttttcta tgcgttgatg acgagtcatc
     4861 tgtatccgga gtgggtggcc cgcgggtttt ttgtctggct gtcggtgttc aacctgttca
     4921 tcgtgtcggt cttctggagt ttcatggacg atctgtttac accggagcaa ggggcgcggc
     4981 tgttcggcgt gatcgccgcc gggggaagca gtggagcgtt gctcggcccg ctcctcacca
     5041 cgggcctcac gtttgtcttc ccgattcagg tgctgatggt ggcatcgagc ctgtttttag
     5101 gagcctgcat cggatgtatg taccgattgg agagatgggg gcggacacag actctccatc
     5161 atcggcccca gcagggtgag ccgctccgcg gcagctttct cgcaggcgtc cgactaacct
     5221 tctcgtcgcc ctacttgctt ggcatttgcg gctacctggc gattctgact atgaccgcca
     5281 cagtccttta ctttgagcag acgcgcttag tctcggaata tctcgaccga ccggaggccc
     5341 ggactcggct gttttccacc ttggacttta cgacaagcct cctgacttgg ctcacgcagg
     5401 tattcatgac cagaagactc atcggccggt tcggtcttgt ggcggcgtta ctgtccttgc
     5461 cggcaatcag tctggtggga ttcttgggga ttgccttgtg gccgagcctc gcggtctatg
     5521 ttgtcttttc cgtgttgcgg agggtggggg aatacgccct gtccaaaccg gcacgggagg
     5581 ttctgttcac ggtggtcagt cgtgaagaaa aatataaggc gaagaacttc atcgacacgg
     5641 ctatctcgcg tggtggcgac gcatcgacgg cctggctcgt gacggcggtg aaggccgtcg
     5701 gtgccacacc cactcacatt gcctggacct tggttccgtt gatgggagtc tgggcctggc
     5761 ttgccacggt actggctcgc caagaaaagc accggtcggc gtctacgtta tcttcgacga
     5821 ccgagaattc tcgcagtaca gcatgagtcg gtaccgcttg cttcggactt tattgcctaa
     5881 tctcgaatga ccagcgagcc gatcacgtaa gaactggctc gtcccgactt ggttcctcgg
     5941 atccgaacct ccgacacatc gcgtacctgg ttatggatgt tccaggtgcg gccggccttc
     6001 aactccggcc agaaccacca ggttccatcg cgcttccgga accagagttg aatcgaggta
     6061 tcgggcaata tcgaaatcga ttccatatca aaatacatgg gcgcatgatt gtcgacattg
     6121 ccgacccaga ttttgatcca ctgattctga tcgtgggagg agaggacgcg gggagtcttc
     6181 gtccaatcga catcggtgca tttaaagtcc accagtttgc cgaaccctcg accgagcccg
     6241 tctcccggat ccggaagggt atagcactcg acccctgttt ccatgttcaa atggaaatgg
     6301 gtgtggccga tagtgtcgaa gttgacatcg cttcgtgcac aacgttggcg gtaggcccag
     6361 cagtcgctat gtttcgacgg atcacacatc tgcaagggcc cgaatccgat tcggctcgtc
     6421 ctgaatgcca gcgttgttct ggcttggccg aagatatgtt ccagcgcgtt ctcatcgtcg
     6481 aaattgatca ggcattgctg tgggctgctg ttgttggggc gggcttgcac gactttggga
     6541 ggttgaccct cccgttctcg ttcggccgat tg
//
LOCUS       DATMTI010000842         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_202438, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000842 DATMTI010000000
VERSION     DATMTI010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202438"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VN039_13280"
     CDS             <1..312
                     /locus_tag="VN039_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWV46971.1"
                     /translation="LPLFGIGMPGHFLVKYESDRYKVFVDCFNGGALLTEKNCARFLT
                     EAGYGFDDKYLQKSPVRAILSRMIKNLLAIYSKSGDGGKTARMTTFIEILGGAPREEG
                     L"
     gene            complement(313..1068)
                     /locus_tag="VN039_13285"
     CDS             complement(313..1068)
                     /locus_tag="VN039_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV46972.1"
                     /translation="MRLTILGSGTNVHPTRAAAGYLVRTDHNILLDFGPRTLMNLIKS
                     GVNRHRITHILFSHFHADHFSDFITFFFDAVIYTKYGGGRRPGMTLIGPKGTIRLLRS
                     IMRSFPSFSPAPFRVVFKEVSGKPFSIGDTRIIPKPVVHVPDLSCVGYRIEYRGKAMV
                     YSGDSQYCDALVRLCADADLAVLDCSFPANRPGLAHLHAGQCGRIARESGLGQLVLSH
                     FYPIADRYDVKAQAGDQYGGKIWKGKDLLTIRI"
     gene            complement(1096..1356)
                     /locus_tag="VN039_13290"
     CDS             complement(1096..1356)
                     /locus_tag="VN039_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46973.1"
                     /translation="MLEQAEPSVSAAEQCFAEAVKMAQEQHAKMLELRAATSLARLLR
                     KLNRLDAAKLVLHSVCSRFREQGANPDLIEAQTILDQLSVVL"
     gene            complement(1368..>4422)
                     /locus_tag="VN039_13295"
     CDS             complement(1368..>4422)
                     /locus_tag="VN039_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWV46974.1"
                     /translation="IAWDVLPELNEGDLEALGVLLGHRKKLLRAIAQLSKRGEGDTAA
                     TPAIPTAIETTSSSSGRNQAERRQLTVMFCDLVGSTALARRLDPEDLQEIIRRFLDAC
                     GKAIGRFDGYVAKYMGDGMLVYFGYPHAHEHDAERAIHAGLSILELARTCSRDHPSTQ
                     ESEIAVRIGIATGQVIVGEIIGQETAKERSVFGETPNLAARLQGLAAPNQLVVDSTTK
                     RLVGSEFEFADQGTVSLKGFEMPAQVWQVLGSKPSASRFESYRAGRLTQFIGREHETA
                     LVLGRWREAVDGEGQVVLLCGEAGIGKSRLVRYLRDRLGEDHYEAIQFQCSPHHTNTA
                     LYPVITNLRQAAGLTDEDNASTQLHKLDTLMADSGPNDRVMIALLADLLSIRGDEQDQ
                     LLNVSPDKRKDMTLEALVQYLQKLADRSPVLLIVEDVHWLDPTTLDLITRIIDRIRQM
                     RVLLLITFRPDFRPVWAEYSHVTFLTLSRLPRRQSAELVLAKTGGKALPLEVQQAILA
                     KADGVPLYIEELTENLLETGMLAEGTDSFTLKAPLREMVIPDSLQALLMERVDRLGPV
                     KEIVQIGAAIGREFTYELLRAAVHVTDAQLNSALDLFVASGLILQEGERSLSRYRFKH
                     MLVQEAAYNTLPKKTRRLLHARIAKTLEEKFADRVQLEPEFLAYHFEQAGLTRPAITY
                     WRLAARRDVDRSANVEALNHFHSALTLLKDLPADTERDALELELLIARGAPMVTVKGY
                     ASPEIEYNYLRARELSRENSYSEHHFLAVWGLWVFHLVRGPLATACDLAEHLLFLAKH
                     EQNQDLLIRAHESIGSTYSFLGRFDEAKAHLLAAKSLYDPARHRPQTLPYAQDPGITA
                     RIMLARTLWILGEVDQVEALLQEAIGMARELEHPFTLAFTLGAVCWISSMLRDADRTL
                     SFADEAVAISTKYSFEVGLAWPMSSQGWALFEKGREEGLTKLVNGLSATQAAGASLNN
                     TYTLALLAELYLRKKRIDEGLGAISEAQKVALTRGNGFGRQNCFD"
BASE COUNT         1006 a         1273 c         1193 g          950 t
ORIGIN      
        1 ctcccgctgt tcggcatcgg catgcccggg catttcttgg tgaagtacga gtccgaccgg
       61 tacaaggtct tcgtcgactg tttcaacggc ggagccttgc tgaccgaaaa aaactgcgcc
      121 cgttttctga ccgaggcagg atacgggttc gacgataaat acctgcagaa aagcccggtt
      181 cgagcgatcc tgtctcgcat gatcaagaac ctcttggcca tctactccaa atcgggtgac
      241 ggagggaaaa ccgcccgcat gaccacgttt atcgagatcc tcgggggcgc cccgcgcgaa
      301 gaagggctct agctagatcc gaatcgtcag gagatccttc cccttccaaa tctttccccc
      361 atactgatcc cccgcttgag ctttcacatc gtaccgatcc gcaatcggat agaaatgcga
      421 cagcacgagt tggcctagac cggattccct agcgatccgg ccgcactgtc cggcatgcag
      481 atgagcgagg ccgggacgat tggccggaaa cgagcaatcg agtacggcaa gatcggcatc
      541 agcacaaagc cggacgagtg catcgcaata ctgcgagtct cctgagtaga ccatggcttt
      601 cccccgatac tcgattcgat agccgacgca cgagagatcg ggaacgtgga ccacgggttt
      661 ggggatgatc cgagtatccc cgatcgagaa tggtttgccc gacacctcct tgaagaccac
      721 gcggaacggc gccggcgaaa aacttgggaa actgcgcatg atcgaacgta agagtcggat
      781 cgtgcctttc gggccgatca gggtcatccc cgggcgacgg ccccctccgt acttggtata
      841 gatcacggcg tcgaaaaaaa acgtaatgaa gtcggaaaag tggtccgcgt gaaaatggga
      901 gaacaagata tgcgtgatcc gatgccgatt cacgccggac ttaatgaggt tcatcaaggt
      961 gcgtgggcca aagtccagga ggatgttatg atccgtacgc actaagtatc ccgctgcagc
     1021 gcgggtcgga tgcacgttgg taccggaacc gagaatggtc agacgcatag gcgaatcagt
     1081 tatacagcca tatggttaaa gaacgacgct aagctgatca agaattgttt gagcttctat
     1141 caagtcaggg ttggcgcctt gttcacgaaa tcgggagcag acggaatgca agaccagttt
     1201 agcggcatcc agccggttca gttttctcaa gagtctggcc aggctcgtcg cggctcgaag
     1261 ctcaagcatt ttggcatgct gctcttgcgc catcttcacg gcttcagcaa aacactgctc
     1321 cgccgcagag acggatggct cggcttgttc cagcaacagt tcccctttca gtcgaagcag
     1381 ttctgcctgc caaaaccatt cccccgagta agggcgactt tctgcgcctc cgatattgca
     1441 cctaaccctt catcaatgcg tttcttgcgc aaatagagtt cggcgagcaa cgccaaagta
     1501 taggtattat tgagactcgc gccggcggct tgcgtggcgg aaagtccgtt cactaatttg
     1561 gtgagacctt cctcacgacc cttttcgaac agagcccaac cttgagagga catcggccat
     1621 gccaacccca cctcaaatga atatttcgtc gaaatggcca cggcttcgtc agcgaaactc
     1681 agcgttcgat ctgcgtcgcg gagcatggag gagatccagc acacagcccc taaggtaaat
     1741 gccagcgtga aggggtgttc caactctcgt gccatgccaa tggcttcctg caacagcgct
     1801 tcgacctgat caacctcacc caatatccat aatgttctgg ccagcataat tctcgcggtg
     1861 atgccgggat cctgagcata ggggagggtc tgtgggcgat gtcgagcggg atcatacagg
     1921 gacttggcgg ccaacaaatg ggctttggct tcatcaaacc ggccaaggaa ggaataggtc
     1981 gagccgatac tctcgtgagc tcgaatcagc agatcctggt tttgctcgtg tttcgccaga
     2041 aacaggagat gttccgccag gtcgcacgct gttgcgagag gccccctgac cagatggaaa
     2101 acccacagcc cccacactgc gagaaagtga tgctcggagt agctgttctc ccgggagagt
     2161 tccctcgccc tgagataatt gtactcgatc tccggcgagg catatccctt cacagtcacc
     2221 atgggggctc cgcgcgcgat gaggagctcc agctccaatg catcgcgctc cgtgtccgca
     2281 ggtaaatctt tcagcaacgt caatgcgcta tggaaatggt tcagcgcttc gacattggct
     2341 gaccgatcca cgtctcgtcg ggctgcaaga cgccaatacg tgatggccgg acgagtcaaa
     2401 cctgcctgtt caaagtggta ggccaggaat tccggttcca actgcacgcg atcggcgaac
     2461 ttctcttcca gagttttagc aatgcgggca tggaggagtc ggcgagtttt tttggggagc
     2521 gtgttgtagg cagcttcttg gacaagcata tgtttaaaac gatatcgtga aagcgatcgt
     2581 tcaccctctt gtagaatgag ccctgaagcg acgaacaaat cgagggcgct gttgagttga
     2641 gcatccgtca catggacggc ggcccgcagc agctcatagg tgaattcacg tccgattgcc
     2701 gctccgatct gaacgatttc cttgaccggc cccaaacggt ccacccgttc catgagcaac
     2761 gcttgcagac tgtccggaat gaccatttcc cttaacggag ccttgagggt aaatgagtct
     2821 gtcccttcag ccagcattcc ggtctccagt agattctccg tcaactcttc gatatacagc
     2881 ggcacgccat cggccttcgc cagaatcgcc tgctgcacct caagcggaag cgcttttcct
     2941 cctgtcttcg ccaacacaag ttcggcgctc tgccggcgcg gaagccgact caacgtcaga
     3001 aacgtgacat gactgtattc cgcccaaacc ggcctgaagt ccggacgaaa ggtgatcagc
     3061 aacaacacgc gcatctgcct gatccggtcg atgattcgcg ttatgaggtc cagcgtggtc
     3121 ggatcaagcc aatgaacatc ctccacgatg agcaacacgg gagaacgatc cgccagcttt
     3181 tgcagatact gcacgagcgc ttccagggtc atgtccttgc gtttgtcggg cgacacattc
     3241 aacagctggt cctgttcatc tcctcggatc gagaggagat ccgcgagcaa ggcaatcatg
     3301 acacggtcgt tgggtccgct gtcggccatc agggtgtcaa gcttatgcag ttgcgtggat
     3361 gcgttgtctt catcggtcag ccccgctgcc tgccgtagat tggtaatcac cggatagagc
     3421 gccgtattgg tatggtgtgg ggagcactga aattggattg cctcgtaatg gtcctcgcca
     3481 agccgatcac gtagatatcg gactagccgc gacttgccga tgccggcctc gccgcagaga
     3541 agaacaacct gtccttcacc gtccaccgct tcgcgccagc gtcccaaaac aagcgccgtt
     3601 tcatgctctc ggcctatgaa ctgggttaat cgtccggctc gataagactc gaaccggctg
     3661 gccgaaggct tactgcccag aacttgccag acctgggccg gcatctcgaa ccccttgagg
     3721 gaaaccgttc cctgatccgc aaactcgaac tcacttccga ccaggcgctt cgtcgtcgag
     3781 tcaacaacca attggttggg tgccgccaac ccctgaagac gggcggccag attcggagtt
     3841 tcaccgaaca ccgaccgctc cttggcggtt tcctgtccaa taatttcccc gacaatgact
     3901 tggcctgtcg caattccgat tcgtaccgca atttcagatt cctgagttga aggatgatca
     3961 cgtgaacagg tcctcgccaa ttccagtatg gacaggccgg catggatcgc tcgttccgca
     4021 tcgtgttcat gagcatgcgg gtacccaaaa tacaccaaca tcccgtcacc catgtatttg
     4081 gcgacatagc cgtcgaatcg accgatcgct ttgccacagg cgtctaaaaa ccgccggatt
     4141 atctcttgaa gatcttcagg gtctaaccgg cgagccaacg ctgtcgaacc cacaaggtca
     4201 caaaacatga cggtcaattg gcgacgctcc gcttgatttc ggccggaaga ggacgacgtt
     4261 gtttctatcg cggtgggaat agccggggtg gccgcagtat cgccttcacc tctttttgac
     4321 agttgtgcga tggcgcgcaa gagttttttt cgatgtccca gtaggacacc cagggcttcc
     4381 agatcaccct cattcagctc gggaagtaca tcccacgcga tc
//
LOCUS       DATMTI010000843         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_245088, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000843 DATMTI010000000
VERSION     DATMTI010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245088"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1284)
                     /gene="hemE"
                     /locus_tag="VN039_13300"
     CDS             complement(259..1284)
                     /gene="hemE"
                     /locus_tag="VN039_13300"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWV46975.1"
                     /translation="MNDRFLRACRREPVDCTPVWFMRQAGRYMSEYRTLRTKHSILEM
                     CKTPELAAQVTLQPIDRFPLDAAIIFADILLPLEPMGISLEFAAGEGPVILNPVRDRA
                     AVDHLKVIDGDELEYVADAIRQARRALDGRVPLIGFAGAPFTLASYAIEGGGSRNYLH
                     TKQMMYGDPTAWHRLMDKFARVLTGYLRRQIKAGAQAIQLFDSWVGCLSAGDYAEYVM
                     PHVQRIFDGLKREGVPMIHFGTGTTAILRQMREAGGDVIGIDWRVHLDEAWTMVGHDR
                     AVQGNLDPLVLFAPLHEIERRVEDILRRAGGRPGHIFNLGHGILPTTPVEHVAATIDM
                     VHKLSQR"
     gene            1563..2618
                     /locus_tag="VN039_13305"
     CDS             1563..2618
                     /locus_tag="VN039_13305"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HWV46976.1"
                     /translation="MSDVQTISPNKAQDFWYSFLRWFDSWAGLEHTKVEGPPKVDWVR
                     SVPFIAVHLMCLGVLWVGWSWTAVAVAVAFYYVRMFAITGWYHRYFSHRTFKTSRTVQ
                     FLFALLGGSCAQRGPLWWAGHHRHHHIASDTPDDVHSPRQGGFLWSHMGWVMSQTHYA
                     PRLKGIADFAKFPELRFLDRFDVLVPTMTGFGMFGFGKLLEAYAPELGTNGPQMLIWG
                     FFISTVALFHGTCTINSLSHVYGSQRYETGDDSRNNFFLALITMGEGWHNNHHYYPAA
                     TRQGFYWWEIDMTYYCLKGLEWMGLVWDIRGVPGYVREGKTKLDSDRSVLKKKIDAAV
                     KAAELPAPQPQLELTPP"
     gene            complement(2743..>3072)
                     /locus_tag="VN039_13310"
     CDS             complement(2743..>3072)
                     /locus_tag="VN039_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46977.1"
                     /translation="IAGEVIRFHNSRFEDDEMAGQVIGDMANQQIGTVNLISLDPHPN
                     LEPHPYVEWFTLRKDHYRFELSPGDAWVLSDEERKTIDGESRRIRETLADRVTDQPRS
                     DDGSEWV"
BASE COUNT          665 a          902 c          825 g          680 t
ORIGIN      
        1 gtatcgttca cgaatctctt ccacgagcgc cggaggtgtc ggtcggcccc ctcgaacgtc
       61 acgcaaaaac ggctcgacgt tctccagaca atcgggccca cccatcgcca tgagcaacac
      121 agcggtatga tgttgcgatt caggcatagg cttgtcgaaa caaatagctg atggctgata
      181 gcggatgata agaactatgc tgctgagcga atgcgcgtcc cgccatcaat catatgccat
      241 ttgccatacg ctcttggttt accgttggct gagcttgtgc accatgtcga tcgtcgcggc
      301 cacgtgttcg accggcgtcg taggcagtat gccgtgaccg agattgaaga tgtgcccggg
      361 gcgtcccccg gctcgccgca aaatatcttc cacacggcgt tcgatttcat ggagcggcgc
      421 aaacagcacg agcggatcga ggttgccctg cacggcgcga tcatgtccga ccatggtcca
      481 ggcctcgtcc aggtgaaccc gccagtcgat cccgatgaca tcgccacctg cctcgcgcat
      541 ctgccgcaga atggccgtgg tgccggtgcc gaaatgaatc atcggcacac cttcccgctt
      601 gagtccatcg aaaatccgct ggacgtgcgg cataacatat tcggcatagt caccggccga
      661 gagacagccg acccaactat cgaagagctg aatcgcctga gcccccgcct taatctgtcg
      721 ccggaggtac cccgtgagca cgcgcgcaaa cttgtccatg aggcgatgcc aggcggtggg
      781 atcgccatac atcatctgct tggtatgcag atagttacga gaccctccgc cctcaatggc
      841 gtagctcgca agcgtgaacg gtgcaccggc aaacccgatc aacgggactc gtccgtcgag
      901 ggcgcgtcgc gcttggcgga tggcgtcggc cacgtactcc agttcgtcgc cgtcgatcac
      961 cttgaggtga tccaccgccg ctcggtcacg aaccgggttg agaatgacgg gcccttctcc
     1021 cgccgcaaac tcgagactga tccccattgg ctccaacggc aacagaatgt cggcaaaaat
     1081 aatggcggca tccaagggaa atcggtcaat cggctggaga gtcacttgag ctgcaagctc
     1141 aggcgtcttg cacatttcca ggatcgaatg ttttgtacgc aatgtccgat actccgacat
     1201 gtagcgtccc gcctggcgca taaaccacac cggcgtgcaa tcgactggtt cgcggcgaca
     1261 ggctctgaga aatcggtcgt tcatagtggc ggcattctag agaggcgtgg gcaaagctgt
     1321 caaacccggt cagcagcatt tctcccccct cagcagacaa ggaatgtgta gacacagggc
     1381 gccgtttcag tacaatcacg tctacagaag ccacgaaaaa actctggttt cactttcctg
     1441 acgttctctg tataataagg actcagccta tcctgcacga ccgatcagct taatcgggac
     1501 cacggctccc tgtctcccac cacagtgacg ttgccgatcc ctcgaccctg gaggagaatt
     1561 gtatgtccga cgtgcaaacg atcagcccga ataaagctca ggacttttgg tattcatttc
     1621 ttcgctggtt tgactcctgg gcaggacttg aacacacgaa agtggaaggg cctcccaagg
     1681 tagactgggt tcggagtgtc ccattcattg ccgtgcatct gatgtgtttg ggagtgttgt
     1741 gggtaggatg gagctggacc gccgtcgccg tcgccgtcgc cttctattac gtccgcatgt
     1801 ttgcgatcac cggatggtac caccgttact tctcccatcg cacgttcaag acttcgcgca
     1861 ccgtccaatt tctctttgcg ttgttaggcg gctcctgcgc tcaacgcggc cccttatggt
     1921 gggcgggtca tcatcgtcat caccacatcg catccgacac gccggacgat gtgcactctc
     1981 cgcgtcaagg agggtttctc tggtcgcaca tgggctgggt catgtcgcaa actcattatg
     2041 ctccgcgcct caaaggaatt gccgatttcg cgaagtttcc ggagctgcga tttctcgacc
     2101 gatttgatgt cctcgttccc actatgaccg gcttcggaat gttcggattc ggcaagttgc
     2161 tggaagccta tgcgcccgaa ctcggcacca acggccccca gatgttgatt tgggggtttt
     2221 tcatctcaac cgtggcgctg tttcatggaa cctgtacaat taattccttg tcacacgtct
     2281 atggctcgca acggtacgag accggtgacg acagccgaaa caacttcttc ctagccctga
     2341 ttaccatggg agaaggttgg cacaataacc accactatta tcctgccgcg acaagacaag
     2401 gcttctattg gtgggaaatc gatatgacct attactgtct gaaaggactt gagtggatgg
     2461 ggttggtctg ggatatccgt ggtgtcccag ggtatgtgcg cgagggaaaa accaagctgg
     2521 attcagaccg cagcgtactc aaaaagaaga ttgatgcagc cgtaaaagca gcggaactgc
     2581 cggcgcctca acctcaactt gaactgaccc ctccctagca cagagtgttc aaaaaggctg
     2641 tccagcaagg ccgcagcaag cgaagaggcg aggcgtacgc ttcggtacgt tgagcctctg
     2701 agcgatgcga gaacgccgct ggcaggcttt ttcaacaccc tgctagaccc actcggaacc
     2761 gtcgtcggac cttggctggt cggttactcg atcggccagg gtctcccgaa tgcgtcggct
     2821 ttccccatcg atcgtctttc gctcctcatc ggacagtacc caggcatcgc caggactcaa
     2881 ttcaaagcga tagtgatcct ttctgagcgt aaaccactcg acgtagggat gcggctcaag
     2941 attcgggtgc ggatccagtg aaatcagatt caccgttccg atctgctggt ttgccatgtc
     3001 gccgataact tgtccggcca tctcgtcatc ttcaaaccgg ctattatgaa atcgaatcac
     3061 ctctcctgct at
//
LOCUS       DATMTI010000844         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_541240, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000844 DATMTI010000000
VERSION     DATMTI010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541240"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VN039_13315"
     CDS             complement(<1..882)
                     /locus_tag="VN039_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46978.1"
                     /translation="MSPFTKIQSVLAKPFMPAVFFLSGVTYDTVTLTRIDRLQDNLIL
                     MLYLAILGTLIVLIGRLGIESPPDREQLASLSSFGRWVLTSRPYYPMASQFLLGGLFS
                     AYTIFYSRSTSLTGTAVFFSLLIILLVANEFLRDRLSNLRLLVSLYGVVTFAFLTFFL
                     PVMTGYMNAVVFIVGAVVSVAVTLRVVQLIYRNNPDRSRHEAIKMTVPTVACIVLLVG
                     FYFMNWIPPVPLSLKFGGMYHEIKRTGDQFELSFERRWYQVWKRSDTTYPSNQPIYCF
                     TAVFAPVDLNTTIYHHWY"
     gene            complement(1035..2096)
                     /locus_tag="VN039_13320"
     CDS             complement(1035..2096)
                     /locus_tag="VN039_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46979.1"
                     /translation="MRERRWETKTSLSFSQALAVGDRLAALGLRPVSPASDVICYVEE
                     WTVGSLDDFDQLDPWATEDVTLVHVRERWRGDFFLLSGAYHTVYQRHQDIGTYCSISH
                     PWRIREILHLHDQNAMLWIGFRHAHSFIRVRLQTQVVITPGETRGDADRTRWLDERRA
                     AFLDAITVLDLPIETHVEKHGVVLRSSNASIPFFCSWPDAFGPCQFEYNTSDAFEFLV
                     PASKLAETFHPEPARVRAYLTGFSEAALADFQEIDPGARLVYRCSVHCPLDDLPEVQN
                     AIQPHGLLYATLCEFQTQAVLPDAEDASAIIGIVGVNGQFKIEARLNHAPLHEAAMLP
                     WLERLIDHPVVYAPLPAFV"
     gene            complement(2178..>2718)
                     /locus_tag="VN039_13325"
     CDS             complement(2178..>2718)
                     /locus_tag="VN039_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46980.1"
                     /translation="VQLTDIDPVKIAIEPFDDRRADKGRVGTRTHLWGGTTQFDVSGD
                     RLAGLITQRLADRLRTRGWRDRVWNVRVASAGSAADADIVINGQVQDFSATVKSRVFS
                     TVIDTSSRFTIQARNLVDRSTTIRTIEGGRSRTVFWFNEDDVREQLAATLKDGLDRLI
                     VDTTIDNKALRPSRRLLQE"
BASE COUNT          663 a          766 c          770 g          519 t
ORIGIN      
        1 gtaccaatgg tgataaatgg tggtgttgag atcgaccggt gcaaagacgg cggtgaagca
       61 atagatcggc tggttcgagg gataggtcgt atctgaacgt ttccagacct gataccaccg
      121 cctctcaaag gagagctcaa actggtcgcc ggttcgcttg atctcatggt acattccgcc
      181 gaacttcagc gatagcggaa ccggaggaat ccaattcatg aagtagaagc ccaccaggag
      241 gacgatacat gcaacagtcg gaacagtcat ctttatcgct tcatgtctcg atcggtcggg
      301 attattgcga tagatgagct gaacaacccg caaggtcacc gccacgctga cgaccgctcc
      361 gactatgaaa accacagcgt tcatgtagcc cgtcatcacg ggcaaaaaga acgtgaggaa
      421 ggcgaatgtc accaccccgt ataggctgac caacaagcga agattcgaca ggcggtcacg
      481 taaaaactcg ttggccacca agagtatgat aagcagggaa aagaagaccg ccgtgccggt
      541 gagcgaggtg cttcgtgaat agaaaatcgt ataggcgctg aagaggcccc cgagcaagaa
      601 ctgactcgcc atagggtagt aaggacggct cgtcaatacc cagcgaccga aagaggaaag
      661 cgacgccagc tgttcgcgat ctggtggaga ttcgatcccc aatcgtccaa tcaagacaat
      721 caaggtacca aggatggcaa gatacagcat cagaatcaag ttgtcctgca gccggtcgat
      781 tcgcgtgagg gtgacggtgt cataggtgac acccgagaga aagaagacgg ccggcatgaa
      841 cggtttcgcg agcacagact ggatcttcgt gaacgggctc atgtcaccac tgtgcaaaat
      901 cggcaggaat tgatcaacca aaatctggtt cgggtgttgg gcgattcagg atcaagccga
      961 ggtcaaggtc gaggtggaac acgtgcatcg tttactaaaa accttagcct cagccttgac
     1021 ctgaagtatg tgcgctaaac gaacgccggt agcggggcat acacgacggg gtgatcaatc
     1081 agccgctcca gccatggcaa catcgccgct tcgtggagcg gagcgtgatt gaggcgcgct
     1141 tcgatcttga actgcccgtt gactccgacg atgccgatga tcgccgaggc atcctctgcg
     1201 tcaggcaaca ccgcctgcgt ctggaactcg cagagcgtgg cgtacaggag accgtgcggt
     1261 tgaatcgcgt tctgcacctc cggcagatca tccagcgggc aatggacgga gcagcgatag
     1321 acgagccgcg ctcccgggtc gatctcctgg aagtccgcca aggctgcttc tgaaaacccc
     1381 gtgagataag cacgaactct ggccggttct ggatggaacg tctccgcaag cttgctggcc
     1441 ggcacgagga attcaaacgc atcggacgtg ttgtactcga actgacaagg accaaacgca
     1501 tcgggccatg agcaaaagaa cggaatggaa gcattggaag accggaggac caccccatgt
     1561 ttctccacat gggtctcaat cggcaggtcc aaaacggtga tcgcgtcgag gaaggcggct
     1621 cgcctctcat ccaaccagcg tgtccgatcg gcatcgccac gcgtttcacc gggcgtgatg
     1681 acgacctgag tctggaggcg aacccggatg aacgaatgcg catgccgaaa cccgatccat
     1741 aacatggcat tttgatcatg aaggtgcagg atttctcgaa tgcgccaggg atggctgatg
     1801 gaacaatagg tgccgatatc ttgatgacgc tgatagaccg tatgataggc tccggataaa
     1861 agaaagaaat cgccgcgcca tcgttcccgt acatggacga gcgtgacgtc ttccgtggcc
     1921 caggggtcga gctgatcgaa gtcgtcgagc gacccgaccg tccattcctc cacatagcag
     1981 atcacgtcgc tggcaggcga gaccggcctt aacccgagtg cggcgagccg gtcccccacg
     2041 gcaagcgcct gactgaaact caaggacgtc ttcgtttccc accgccgctc acgcaccgtg
     2101 agggatcctt gaagacaggt tgaggttgtc gtcaaccctc aaaaaatcaa tgcccctctc
     2161 cactgggtcg caacggttca ctcttgcagg aggcgccgtg acgggcgtag cgccttgttg
     2221 tcaatcgtcg tgtcgacgat cagccgatca agtccgtctt tcaaggtcgc cgccaattgt
     2281 tctcgcacat catcctcatt gaaccaaaac accgttcgac tccgcccccc ttcaatggtg
     2341 cggatggtcg tgcttcggtc gacaaggttc ctggcctgaa ttgtaaaccg gctgctggtg
     2401 tcaatcacag tggaaaaaac gcggctcttc acggtcgcgg agaaatcttg cacctgtccg
     2461 ttgatgacaa tatcggcatc cgcggcagat ccagccgagg ctacacgcac gttccatacc
     2521 cgatctcgcc acccacgagt tcttaaccga tcggccagtc gctgagtgat caagcctgca
     2581 agccgatcac ccgacacatc gaactgcgtg gttcctcccc agagatgagt gcgcgtcccc
     2641 acacggccct tgtccgcccg tcgatcgtca aacggctcga tggcgatctt gaccgggtcg
     2701 atgtcggtca gctgcacc
//
LOCUS       DATMTI010000845         7792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_115892, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000845 DATMTI010000000
VERSION     DATMTI010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115892"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..325
                     /locus_tag="VN039_13330"
     CDS             101..325
                     /locus_tag="VN039_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46981.1"
                     /translation="MDTPQKRFDTQYITSSQIRERVQVSRVTLHQRRSSGKLPGAIEL
                     GDRQTFIWERESIEPHIREWEIALHARRAG"
     gene            322..2724
                     /locus_tag="VN039_13335"
     CDS             322..2724
                     /locus_tag="VN039_13335"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HWV46982.1"
                     /translation="MSQEARWGHIPLELRQRQQWCFTQPGSKAPRGRGNYLVSDTEPS
                     QWMTFEEACGLAAFYKGAIGYCLTPDDPYTCIDMDVKDASNCPEHPDKWTTQAQFDRY
                     WAITQAFNTYTEKSFSGKGLHLWVRGNIGTGARRDGVEVYSQWRFIVTTGDIVVNAKI
                     EERTELLNILVQEIRAAQAAQYEKVDLVEEDEELDDHDLLARAMSAGNAEKFNALCRC
                     TSAVGDGTTKVHGSYIELGYQSQSEADLALMSIFTFYSKSNEQCKRLFRMSGLGQRPK
                     AQDDDRYLNETLRTIRSRQAKEQAAEFVQIQSAANIVQRLAQERGAQLHVPGAGEPVA
                     TAAPASVGVVSIAPVDELAGLNGETDRSLDWPPGLAGHMAYYIYQSSPRPVKEVSIVA
                     ALGLLAGICGKAFNIPQSGLNLYIILIARSGVGKEAMHSGIASLIQAAASRQPPVMSF
                     VDFNDFASGPALNKAVAVNQSFVNVAGEWGRKLRRLALEDGRDGPMQQLRTVMTNLYQ
                     KSGPQSIVGGITYSNRDTNIASVQGVAYSMIGESTPGTFYEALTPSMMEDGFLSRFTI
                     IEYNGMRPPLNHAPLREPSKVLGDAVADLCTHAHTLLSRHESQQVQRSESAATKLQAF
                     EIECDAQINATHDEMWRQMWNRASLKVMRVSALLAVAENWLNPIISDEHVDWAMDLIR
                     RDINIMTRRIDEGDIGIDDSAREKKMMRFIRNYLQYNIGGIDVPEQMRKDGVVPRKIL
                     ASATARINCFMKYRGGATAALNETLKSMCDSGYLIEIDKAQLIAKYAYTGKCYRVLQI
                     RD"
     gene            2733..3302
                     /locus_tag="VN039_13340"
     CDS             2733..3302
                     /locus_tag="VN039_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46983.1"
                     /translation="MRNVTGLPDNLTEYSEKIFSDNVERGWWKRGEDGEVQSRNVGEL
                     LCLIHSEIDEADSDRMDEKLPHRFEVEVELADTAIRIFDMLGFYKVDIDFRRMKLEEP
                     LPLPIGTYRADWLMVLHKYTSTAMEFFRKGNSDVGCTELVKLLGAIHFCAIAFELDLH
                     GAIDEKLQYNAHRADHKLENRQKEGGKAF"
     gene            3389..3904
                     /locus_tag="VN039_13345"
     CDS             3389..3904
                     /locus_tag="VN039_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46984.1"
                     /translation="MNEINKPRTEEERQNLLQEWYKINDALGAMKSKESLLRAAVYRE
                     FFVKKDENGQDILPEEGTNKFSLPHDYVLKAQRVINRTVDAAALKQLSHTKAELEYKI
                     ANGITLSDEEALQHKVLKGILLEELIKYKPELAIKEYRTLTVDEQRVFDDVLVIRDGS
                     PQLEIVLPKRK"
     gene            3908..4390
                     /locus_tag="VN039_13350"
     CDS             3908..4390
                     /locus_tag="VN039_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HWV46985.1"
                     /translation="MRVTVMCDASHCPETHVAGYGFWLASSRGSWGGGGRIVRQVSNP
                     MVAEMMAIANSLWIGLNKGTICSGDHLLIQSDCIGAMDKLTKLVPKTKEEIEVRSYFT
                     KMVCEHKLKVDFRHVRGHTGQKDARSCANHHCDRRAYREMRYAREEFKQAQGIKHDAT
                     "
     gene            4392..5132
                     /gene="thyX"
                     /locus_tag="VN039_13355"
     CDS             4392..5132
                     /gene="thyX"
                     /locus_tag="VN039_13355"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWV46986.1"
                     /translation="MKVLDHGFVILRNLAGPTRRMWNGLAADGDYRYSGEERNFDADD
                     IDPAQCARMSFEQMDSDRTYEQDMKLNEYLLRNLHTSPFEMIQVWLEVKVPIFVDRQM
                     VRHRTWRRNESSGRYVTLPAEWYIPEVVGGKAANKKQGQEDNLDEWKQMEFRTQLLNH
                     CSRGYAEYLRAMAIGVAPEHARMFLSVNHYVHWIGNVDLANLFKFCALRAHSHAQIEA
                     QKYADAIIQLLRPELPGLMRLFDEVVRR"
     gene            5129..5590
                     /locus_tag="VN039_13360"
     CDS             5129..5590
                     /locus_tag="VN039_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46987.1"
                     /translation="MIKQKQLNRHVPAEDIIGDCYRTTIACLLDKHPSEVPHFGELCW
                     DFEKKEFREGSDMDAEAEKYLQTIGYTLVRVAWVFDSVQEMLECQEKLNPGVRYILAG
                     RSRNEVNHVVIASDGEIEWDPSQNDSGIIGPCSDGFYWATYILPSFMRKVA"
     gene            5587..6522
                     /locus_tag="VN039_13365"
     CDS             5587..6522
                     /locus_tag="VN039_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46988.1"
                     /translation="MSVLMLKPMLASKVERDKLTFPAYMSPKLDGVRCLITGPGIAMS
                     RSMKAIPNEYVQSVLGREMIVGLDGELCVGPYNAQNLMQATTSGVMSEDGEPDFTFYV
                     FDYFNSIANEPYDVRINKLVRAFETLDKWAGDVVARHVKLLPQALVNSIEEMDRFEQI
                     CLEHGFEGAMYRKVNGAYKFGRSTAREGHLLKLKQFLDGEAVVIGAEELCKNENELTT
                     DELGYAKRSSHKDGKVPIDSLGAFICRDLKSGIEFNIGTGLGLTLELRKRFWEMHKRG
                     ELVGKIVTYKSQHVGVKEKPRIPVWHAMRDPKDMS"
     gene            6583..7320
                     /locus_tag="VN039_13370"
     CDS             6583..7320
                     /locus_tag="VN039_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV46989.1"
                     /translation="MALQFTTAEHESTTHGIKCMVYGPAGMGKTVLNATMPNPVMISA
                     ESGALSLSQKNLERIFGVGHPWVTYNMPIIVVKTIDDLKDAFRYLTESAEATQFQSCG
                     LDSLSEIAEVVLNAAKRTVKDPRQAYGELIEKMETVVRDFRDIKGKNVLFLSKMEPMK
                     DELTGVVKYGPMMPGAKLGVKMPYFFDEVFRYGISKAPDGSEFRFLQTQPDLQYEAKD
                     RSGALDKTEYPMMWPIFDKIKGVQPHG"
     gene            7313..>7792
                     /locus_tag="VN039_13375"
     CDS             7313..>7792
                     /locus_tag="VN039_13375"
                     /inference="COORDINATES: protein motif:HMM:NF016896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HWV46990.1"
                     /translation="MAELNLDFTNVAPDTGFELVPDGWYNLMVDESEIKPTKDGTGAY
                     LNVRITVIDGPMANRKIFTMFNIRNANPVAQEIAQKQLSALAHAVNVLVVKSSEQLHG
                     IPFKGKIKTQKGGLIDKNDKDGGRYEDRNIISVYKNINEQVGDTAGGANAQAAGASKA
                     "
BASE COUNT         1900 a         2121 c         2231 g         1540 t
ORIGIN      
        1 cgcgacggtt aagctggggt tgtgatttgt aattaacgcg tgtaaggtat gccgctaccg
       61 cgcaacctgc gcagtgccgt tcaccgagtc ggaagaagat atggatacgc cgcagaagcg
      121 attcgacact cagtacatca catcgtcaca gattcgggaa cgagttcaag tttcacgtgt
      181 caccctccat cagcgccgct cgtccggaaa gttgcctggc gcgatcgagc ttggtgacag
      241 gcagactttc atctgggaac gcgagtccat cgaacctcac attcgtgagt gggagatcgc
      301 attgcacgcc cggagggccg gatgagtcaa gaagccaggt ggggacacat tccacttgag
      361 cttcggcaac gtcagcaatg gtgtttcact caaccaggca gcaaggcacc gcgcggtcga
      421 ggaaactatc ttgtatctga caccgagcct tcgcagtgga tgacattcga agaagcctgt
      481 ggtctcgctg ccttctacaa gggagctatc ggctattgcc tgacgcccga cgatccgtac
      541 acatgcatcg acatggacgt caaggacgcc agcaactgtc ccgagcatcc agacaagtgg
      601 acgacgcaag cacagttcga tcgatactgg gccatcacac aagcgttcaa cacttacacg
      661 gagaagtcat tctctggcaa aggcctgcat ctgtgggtac gcggtaacat cggcacaggt
      721 gcacgtcgcg acggtgttga agtctattcg caatggcgtt tcatcgtcac aactggcgac
      781 attgtcgtca acgcgaagat cgaagagcgt acagagctcc tgaatatcct cgtccaggag
      841 atacgcgctg cgcaggccgc acagtacgaa aaggtcgacc tcgtcgagga agacgaggag
      901 ctggatgacc acgacctgct cgcccgtgcg atgtccgcag gcaacgcaga gaagttcaac
      961 gcgttgtgca gatgcaccag tgcggtcggt gatggaacga caaaggttca cgggtcatat
     1021 atcgaactag gctatcaaag ccagtccgaa gccgaccttg cgctgatgtc gatcttcaca
     1081 ttctatagta aatcgaacga acagtgcaaa cgtctcttcc ggatgagcgg gcttgggcaa
     1141 cgtcccaaag cgcaagacga tgatcgatat ctgaacgaga cacttcgcac cattcgctcg
     1201 cgacaagcga aggaacaagc tgccgagttc gtccagatcc aaagcgcagc caacatcgtc
     1261 caacgactcg cccaggaacg cggcgcacaa ctccatgtcc ccggtgcagg cgagcccgtc
     1321 gctactgcag cacccgcctc tgtcggtgtt gtgtccatag cgcccgtgga cgagcttgcg
     1381 gggttgaatg gagagaccga tcgcagtttg gactggcctc ctgggcttgc cggtcacatg
     1441 gcgtactata tctatcagtc gtccccgcga ccagtaaaag aagtctccat cgttgcagcg
     1501 ctcggattgc ttgcaggcat ctgtggtaaa gcgttcaaca ttcctcagag tggtctcaat
     1561 ctttacatca ttctgatcgc acgttctggt gtaggtaaag aggcgatgca ttcagggatc
     1621 gcttcgctca ttcaagctgc tgccagcagg caaccgccag tcatgtcgtt cgtcgacttc
     1681 aacgactttg catctggccc tgcgctcaac aaagcagttg ctgtcaatca gtcgttcgta
     1741 aacgtggcag gcgaatgggg acgtaaactc cgacgactcg cactggaaga cggtcgcgat
     1801 ggtccgatgc agcaattgcg aacggtgatg acgaacctgt accagaaatc tggaccacag
     1861 tccatcgtcg gtggtatcac ctactcgaac cgagacacga atatcgcatc ggttcaaggt
     1921 gtcgcttaca gcatgattgg cgagtcaact cctggaacgt tctacgaagc acttacccca
     1981 tcaatgatgg aagacggttt tctgtctcgc tttacgatca tcgaatacaa cggcatgcga
     2041 ccgccgctga atcacgcgcc cctgcgcgag ccaagcaaag tgctgggcga cgcggtagcg
     2101 gatctgtgca cgcatgcgca tacgctgctg agtcggcacg agtcgcagca ggtgcagcga
     2161 agcgagagcg ctgcgacaaa gctacaggcg ttcgagatcg agtgcgatgc gcagatcaat
     2221 gccacgcatg acgaaatgtg gcgtcagatg tggaatcgtg catctctgaa agtcatgcgt
     2281 gtctcggccc ttcttgcggt agcggagaac tggctcaacc ccatcattag cgacgagcat
     2341 gtggactggg cgatggatct tatccgtcgc gacatcaata tcatgactcg acgtattgac
     2401 gaaggcgata tcggaattga cgacagcgct cgcgaaaaga agatgatgcg cttcatcagg
     2461 aattatcttc agtacaatat cggcgggatt gatgtcccag aacagatgcg caaggacggg
     2521 gtcgttccgc gtaagatatt ggcgtctgct accgctcgaa tcaattgctt catgaagtac
     2581 aggggaggcg cgaccgccgc tctcaacgag acactgaaat cgatgtgcga tagcggttat
     2641 cttatcgaaa tcgacaaggc acaactcatc gcgaagtacg cctacacagg caaatgctat
     2701 cgcgtccttc aaatcagaga ctaggaatcg acatgcgcaa tgtgaccggc ttgcccgaca
     2761 atctgaccga gtattccgaa aagatctttt ccgacaacgt cgaacgcggc tggtggaagc
     2821 gcggagagga cggcgaagtc cagtctcgca acgtgggcga gcttctctgt ttgatccaca
     2881 gcgaaatcga cgaggcggac agcgatcgga tggacgaaaa gctgccgcat cgtttcgaag
     2941 tcgaggtcga actggcggac accgcgatcc gcatcttcga catgctcggc ttctacaagg
     3001 tcgatatcga ctttcgtcgc atgaagctcg aagagccgtt gccccttcca attggcactt
     3061 accgcgcgga ttggttgatg gttcttcaca agtacacgtc gactgccatg gagttcttcc
     3121 gcaagggcaa ttccgatgtg ggctgcaccg aactcgtgaa gctgctcggc gcaatccatt
     3181 tctgcgctat cgcattcgaa ctcgacctac acggtgcgat cgacgagaag ctccagtaca
     3241 acgcgcaccg tgctgaccac aagctggaga accgtcaaaa ggaaggcggg aaggcgttct
     3301 aagaaatcag ctaacgcctg ttgcattttg cgctgggccg cctatactaa ccctgtaggc
     3361 ggttccaatt ccatcacagg agtacgcaat gaacgagatc aacaagcccc gcaccgagga
     3421 agagcgtcag aatcttctcc aggaatggta caagatcaat gacgccctcg gcgccatgaa
     3481 atccaaggaa tcgctgcttc gcgcagcggt ctaccgggaa ttcttcgtca agaaagatga
     3541 aaacggccag gacatccttc cggaagaagg aacgaacaag ttctcactcc cgcacgatta
     3601 cgtcctgaag gcgcagcgcg tgatcaaccg caccgtcgac gctgctgcac tgaagcagct
     3661 cagtcatacg aaggcggaac tggagtacaa gatcgccaac ggcatcacgc tcagcgacga
     3721 ggaagctctc cagcacaagg tcctgaaggg catcttgctc gaagaactca tcaagtacaa
     3781 gccggagctg gcgatcaagg agtatcgcac gctcacggtg gacgagcagc gcgtcttcga
     3841 cgatgtcctg gtgatccgcg acggttcgcc tcagctggaa atcgtgcttc cgaagcggaa
     3901 gtgagccatg cgcgtcacag tgatgtgtga cgcgtctcac tgccccgaga cgcacgttgc
     3961 aggatacggg ttctggcttg ccagttcccg tggctcgtgg ggtggcggag gacgcatagt
     4021 acgccaagtg agcaatccga tggtcgccga aatgatggct atcgcaaact cgctctggat
     4081 cggactcaac aagggaacca tctgctctgg cgatcatcta ctgattcagt ccgattgcat
     4141 tggcgccatg gacaagctca caaagctcgt tccgaagaca aaagaggaga tcgaggttcg
     4201 gtcgtacttc acaaagatgg tctgcgagca caagctgaag gttgatttcc ggcatgtgcg
     4261 tggtcatacg ggacaaaagg acgctcgctc gtgtgcaaac catcattgcg atcgtcgcgc
     4321 ataccgagag atgcgctacg cgagagaaga attcaaacag gcgcaaggaa tcaaacatga
     4381 cgcaacataa gttgaaggtt ctcgatcacg gtttcgtcat cctgcgcaat ctcgcagggc
     4441 cgacgcgccg catgtggaat ggtctcgctg cagacggcga ctacaggtac tcaggtgaag
     4501 agcgcaattt cgatgcggat gacatcgatc cggcgcagtg cgcacgcatg tccttcgagc
     4561 aaatggacag cgatcgcacg tacgagcagg acatgaagct gaacgagtat ctgcttcgca
     4621 acctgcacac gtcgccgttc gagatgatcc aggtctggct tgaagtcaag gtcccgatct
     4681 tcgtggatcg ccagatggtg cgccaccgca cttggcgtcg caacgagtcg agcgggcgct
     4741 atgtcacact tccggcggaa tggtacatcc ccgaagtcgt tggcggtaag gcggccaaca
     4801 agaagcaggg gcaggaagac aatctggacg aatggaaaca gatggagttc agaacgcagc
     4861 ttctcaatca ctgttcgcgg ggttatgctg aatatctccg cgcgatggca attggcgtcg
     4921 cgcctgaaca tgcgcgcatg ttcctgtccg tgaaccatta cgttcactgg atcgggaatg
     4981 tggacctcgc gaacttgttc aagttctgcg cactccgcgc ccacagccac gcgcagatcg
     5041 aagcgcagaa gtacgccgat gcaatcatcc agctgcttcg tccggagctt cccggtctga
     5101 tgaggctctt tgacgaggta gtgcgtcgat gatcaagcag aagcagctca atcggcacgt
     5161 tccggcggaa gacattatcg gagactgcta ccgcaccaca atcgcctgtc ttctcgacaa
     5221 gcatccgagt gaagtgcccc atttcggcga gctctgctgg gacttcgaaa agaaggagtt
     5281 ccgcgaaggt tcagatatgg acgccgaagc ggagaagtat ctacagacca tcggctatac
     5341 gttagtccgg gttgcctggg tattcgactc cgtgcaggag atgctggaat gccaggaaaa
     5401 gctcaatccg ggcgtgcgct acatcctggc tggccgtagc agaaacgaag tcaaccatgt
     5461 ggtcattgcg tcggatggtg aaatcgagtg ggatccgtcg caaaacgatt ccggcatcat
     5521 aggtccctgc tctgacggct tttactgggc gacgtatatc cttccaagtt tcatgaggaa
     5581 agtggcatga gcgtactgat gctgaaacca atgctcgcct cgaaggtcga gcgtgacaag
     5641 ctcactttcc ccgcttacat gtcgcccaag ctcgacggcg tgcgatgcct gattacaggt
     5701 cctggcatcg caatgtcccg cagcatgaag gcgattccga acgaatacgt ccagagcgtc
     5761 cttgggcgtg aaatgatcgt gggcctggac ggcgagctgt gcgtcggccc ctacaacgcg
     5821 cagaatctga tgcaagcaac gacgtccgga gtgatgtcgg aagatgggga accggacttc
     5881 acgttctacg tgttcgacta tttcaactcc attgcgaacg agccttacga cgtgcgcatc
     5941 aacaagctcg tgcgagcatt cgagacgctc gacaagtggg caggtgatgt cgttgcgaga
     6001 cacgtcaagc tactgccgca agcactggtg aacagcatcg aggagatgga tcgcttcgag
     6061 cagatctgcc tggagcacgg tttcgaaggt gccatgtacc gcaaggtgaa tggcgcatac
     6121 aaattcggac gcagcacagc gcgtgaagga catctcctga agctgaagca gttcctggac
     6181 ggcgaagctg tggtcatcgg agcagaggag ctctgtaaga acgagaacga gctgaccaca
     6241 gacgaactcg gctatgccaa gcgaagcagc cacaaggacg gcaaagttcc gattgattcg
     6301 ctgggagcgt tcatctgtcg agacctcaag agcggaatcg agttcaacat cgggactggc
     6361 ttgggactca ctctggagct gcgaaagcgc ttctgggaga tgcacaagcg cggggagctc
     6421 gtcggaaaga ttgtcacgta caagtcgcag catgtgggcg tgaaggaaaa gccccgcatt
     6481 cctgtctggc atgcgatgcg cgatcccaag gatatgagtt aagtgcttgt aatccgaagc
     6541 attttccgct aagctgcatt tctcgtttac aacggagcgc atatggccct tcaatttacc
     6601 acagcggaac acgagtcgac cactcatggc atcaagtgca tggtgtacgg tccggcgggt
     6661 atgggcaaga ccgtgctcaa cgccaccatg cccaaccctg tcatgatcag cgcagaatct
     6721 ggtgcgctgt ccctctcgca gaagaatctc gaacgcatct tcggtgtcgg ccatccttgg
     6781 gtcacgtaca acatgccgat catcgtcgtc aagacgatcg acgacttgaa ggatgccttc
     6841 cgatatctca cggagagcgc ggaagcgaca cagttccaat cgtgcggtct ggactctttg
     6901 tccgaaatcg cagaagtcgt gctgaacgct gcgaagcgta cagtgaaaga ccctcgccag
     6961 gcctatgggg agctgatcga gaagatggaa acggtcgtcc gtgacttccg tgacatcaag
     7021 ggaaagaatg ttctgttctt gtccaaaatg gaacccatga aggacgagtt gacaggtgtc
     7081 gtgaagtacg gccccatgat gccgggtgcg aagctcggcg tcaagatgcc ctacttcttc
     7141 gatgaagtgt tccgttacgg gatcagcaag gccccggacg gttcggagtt ccggtttctg
     7201 caaacgcaac cggatctgca gtacgaagca aaggatcgtt cgggtgctct ggataaaacc
     7261 gagtatccga tgatgtggcc cattttcgac aaaatcaaag gagtacagcc ccatggctga
     7321 gttgaacctt gacttcacaa acgtcgctcc ggacactggc ttcgagctcg tgccggacgg
     7381 ctggtacaat ctgatggtgg acgagtccga gatcaagccg acgaaggacg gcaccggcgc
     7441 ctacctgaac gttcgcatca ccgtcataga cggcccgatg gcgaaccgca agatcttcac
     7501 gatgttcaac atccgcaacg ccaacccggt cgcgcaggaa atcgcccaga agcagctgtc
     7561 ggctctggcg cacgcggtga acgtgctcgt ggtcaagagc tccgagcagc tccacggcat
     7621 cccgttcaag ggcaagatca agacgcagaa gggcggtctg atcgacaaga acgacaagga
     7681 cggcggccgg tacgaagacc gcaacatcat ctcggtctac aagaacatca acgagcaggt
     7741 cggcgacact gccggcggtg cgaacgcgca ggcagcaggg gccagcaaag cc
//
LOCUS       DATMTI010000846         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_31549, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000846 DATMTI010000000
VERSION     DATMTI010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31549"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VN039_13380"
     CDS             <1..406
                     /locus_tag="VN039_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46991.1"
                     /translation="VAGVRNAPLAWDTAENRGRAEIAKTFETYTGYLMRDYAASTTAG
                     DFTRNTEEQNVERAIKTITTTTLSGVRKIDQYMDPKTNTYYVLTKLSLEDMKNNLEQA
                     KELNSQVRDFVRRNADRLFERLEQEEEKRGVR"
     gene            451..1062
                     /locus_tag="VN039_13385"
     CDS             451..1062
                     /locus_tag="VN039_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HWV46992.1"
                     /translation="MIQRHRPSLVAMMSIALALSGCGHETKVTRVESEVVIDLSGRWN
                     DTDSRMVAEAMVKDALQYPWLGNFEKASQRQPVVVVGTVLNSSHEHINVQTFITDLER
                     ELTNSQKVTFVAGKGERDEVRSERKEQAIYAREETQKAPGKETGADFMMKGTIATILD
                     EADGTKAIFYQVDLQMIDLENNAKVWYGQKKIKKVVERKRTIF"
     gene            1075..2538
                     /locus_tag="VN039_13390"
     CDS             1075..2538
                     /locus_tag="VN039_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46993.1"
                     /translation="MLNPILPRLAHGPISSLPLLAVLVLLSGCGPSVNRYLLIEQSLV
                     AGDPQRASAIVEQTEKEYGAKGRLLYEMDRGMVLQLAGRYQQSSAALEQAEEEVERLY
                     TRTIRSETAAFVTNDNALPYEGDAYEHVMINVIKALNYAVQGQLQEALVEARRIDHRL
                     NVLSDRVTDADQYRNDGFARYLSGILYEATGDLNNAFIAYRNAYEAYGSMQGWLKMSF
                     PPSLRSDLLRTAEALALTTEFAEYRQAFPDVEWHSSSSQEQLAQVVMISYNGRAPRKE
                     ELFLDLPISLDALQLVLINRGVFQSPYQRNRAVDSVLYGLNGQVVRVALPRLVPQKTK
                     VAFENMTLTDSAGHSFSVRSELVHNVTALAEKSLSKRLPAITVKALARAATKYAIAEG
                     ITRSAQQAAGRDGAPWVGLLVALLTHGLAVASEEADKRSWQTLPDEIHVARVWVSPGQ
                     YQMSIRPSGQAVPTAKDGQTIALTAGQTMFIIQRVMQ"
     gene            2535..3674
                     /locus_tag="VN039_13395"
     CDS             2535..3674
                     /locus_tag="VN039_13395"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HWV46994.1"
                     /translation="MMRAGQPSVGQGIAVFLCLWLTMSAVTACTWFTGKGKPGWVDGR
                     SAEYPSTQYLTGVGQADTRSNAEDQAYAAVARIFKAEVAAQAKDWESYWVVENHGVSN
                     AERRLTIDNVTKVSTAKVLENVRIADVWYDSDSRSYHALGVMNRSQAEAALMEKVSAS
                     DRAIDADVTESRQTTDKLAKVRALRRAGRNLVLREVYNTDLRVIRPSGQGTPSVYQVN
                     ELSGELEQFLATNLVIAVQVSGDHAEPVGRALEEGLIREGLQVTTKPEGEGTGSPEVI
                     VRGTVRLFPVEVRDPNFKYVRWCSDFEVVEPGTGRILGAVAQGGREGHLTEREATAKT
                     LRVMQQELSSDVAKAIAAHIFGEVSLPETATMPAGCPREESGVKR"
     gene            3711..3980
                     /locus_tag="VN039_13400"
     CDS             3711..3980
                     /locus_tag="VN039_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46995.1"
                     /translation="MKKTDELPVNRSPRSRGRGLTKSGQKVSSRLAKRRLNDRLKEDT
                     SSFNRGNLADTLRKEGYEEVPLDELQDRLSKLQGPLAEIILKGRV"
     gene            3985..4428
                     /locus_tag="VN039_13405"
     CDS             3985..4428
                     /locus_tag="VN039_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWV46996.1"
                     /translation="MPYYYFDSTALIKRYSMERGTRIVNKLMVKRGKVAIVPTWTVTD
                     FYSILCARAQEGQITRDDCYSVLYKFEIESKEGLYQFIAPRMETYLATKELVLEYPSL
                     RSPQVMHLALALELKPLRLTVVSADTQLLAASKTAGLHIINPAED"
     gene            complement(4447..>4644)
                     /locus_tag="VN039_13410"
     CDS             complement(4447..>4644)
                     /locus_tag="VN039_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV46997.1"
                     /translation="PLLLTVDRPVLVQKPDGSYDPDINLPNLIRAPGIGPAGWNHAVM
                     ELLGQASEETSPGRSVNIQAT"
BASE COUNT         1096 a         1160 c         1436 g          952 t
ORIGIN      
        1 ggtcgcgggg gttcgtaatg cgccgcttgc gtgggatacg gccgagaatc gcgggagggc
       61 cgagatcgcc aagacattcg agacctatac cgggtatctg atgagagact atgcggcctc
      121 gacgaccgcc ggagatttta cccggaatac ggaagagcag aatgtcgaga gagccatcaa
      181 aaccattacg acgacgacgc tcagcggtgt ccggaaaatc gatcagtaca tggatcccaa
      241 gaccaacacc tattatgtgt tgaccaagtt gagtttggag gatatgaaaa acaacttgga
      301 acaagccaaa gagcttaatt cgcaagtacg ggacttcgta cggaggaatg cggaccgtct
      361 gttcgaacgg ctggagcagg aagaggaaaa gcgaggtgtt cgctaaccta catggtgaat
      421 gagctgtcca cctctttgcg gaggtttgct gtgatccaga ggcatcgccc ttcgcttgtc
      481 gccatgatga gcattgccct cgctttgtca ggatgcggac acgaaacaaa ggtgacccgc
      541 gtcgagagcg aggtcgttat cgatttgagc ggccggtgga atgatacgga ttcccggatg
      601 gtcgccgaag cgatggtcaa ggatgcgctg caatatccgt ggctgggaaa tttcgaaaag
      661 gcgagtcagc gtcaacccgt cgtcgtggtg ggaacggtcc tgaacagcag tcacgaacat
      721 atcaacgtgc agaccttcat tacggattta gagcgagaac tcaccaattc ccaaaaagtt
      781 acgttcgtgg ccggtaaagg cgagcgcgac gaggttcgaa gcgaacggaa agagcaagcc
      841 atctacgcac gcgaagagac gcagaaggcc ccaggaaaag agaccggggc cgacttcatg
      901 atgaagggta cgatcgccac cattcttgat gaagctgatg ggaccaaagc gatcttctac
      961 caagtggatc ttcagatgat cgatctggag aacaatgcca aggtctggta cgggcagaag
     1021 aagatcaaga aggtggttga aaggaagcgt actatctttt aggtttctcg gacgttgttg
     1081 aaccccatcc tcccacgcct tgcccacggg ccgatctcgt cgctgccgct tcttgccgtt
     1141 ctcgtgctcc tgagcgggtg cggtccttcc gtcaatcggt acctcctcat tgaacaaagc
     1201 cttgttgccg gtgatccgca acgggcctcg gcgatcgtcg agcaaaccga gaaagagtat
     1261 ggagccaagg ggcggttact gtatgaaatg gatcgaggaa tggtgctgca gctggcaggc
     1321 cgatatcaac aaagcagtgc ggcattggaa caagcggaag aggaggtcga gcggctctat
     1381 accaggacca tccgttcgga aaccgctgca tttgtcacca acgacaatgc gttgccgtac
     1441 gaaggcgacg cctatgagca cgtcatgatc aacgtgatca aggcattgaa ctatgcggtt
     1501 cagggacaac ttcaggaagc actggtggaa gcgcgacgta tcgaccatcg cttgaatgtc
     1561 cttagtgaca gagtgacgga tgccgatcag tatcggaatg acgggtttgc ccggtatttg
     1621 agcggtatcc tgtacgaagc aaccggtgat ctcaacaacg cgtttattgc ctatcgcaat
     1681 gcatatgaag cgtatggatc gatgcaaggg tggttgaaga tgtcctttcc cccttccctg
     1741 cgctcagatc tcttgcgcac agcggaggcg cttgccttaa ccacagagtt tgccgagtat
     1801 cgacaggctt tccctgacgt tgaatggcac tccagctcat cccaagagca gctcgcccaa
     1861 gtcgtgatga tcagctataa tggtcgggct ccgcgcaaag aggagctgtt cttggatcta
     1921 cccattagtc tggatgcgct gcagttggtc ttgatcaatc gtggagtctt ccagtctccc
     1981 tatcagcgaa atcgcgcagt ggacagtgtg ctctatggac tcaacgggca ggtcgtccgc
     2041 gtcgcgctcc cgaggttagt cccgcaaaaa acgaaggtgg cgtttgaaaa tatgacgctg
     2101 accgattcgg ccggccattc gttcagcgta cgatcggaac tcgtgcataa tgtgacggct
     2161 ctcgcagaga agagcctttc gaaacgcttg ccggccatca ccgtgaaagc tctcgctcga
     2221 gccgcgacga agtatgcaat agcggaaggc atcaccagaa gcgcgcagca ggcggctgga
     2281 agggatggcg ctccctgggt tggactgctg gttgcgctgc tgactcatgg gctggccgtg
     2341 gcctccgaag aagccgacaa gcgaagttgg cagaccttgc ccgacgaaat tcatgtggct
     2401 cgggtatggg tatcgcccgg tcagtaccag atgtccattc gaccgtcggg gcaagcggtg
     2461 ccgacggcga aggatggaca aacgatcgcg ctgactgcag gccagacgat gttcatcatt
     2521 cagcgtgtga tgcaatgatg cgtgccggtc aaccttctgt cggacaaggc atcgcggttt
     2581 tcctgtgtct gtggttgacg atgtccgcag tgacggcctg tacctggttt accggaaaag
     2641 ggaagccggg gtgggtcgat ggccggagcg cggaatatcc atcgactcaa tacctgaccg
     2701 gggtcggcca agcagacact cgaagcaatg ccgaggatca ggcctatgcg gctgtggccc
     2761 gcatcttcaa ggcggaagtt gcggcgcagg ctaaggactg ggaatcgtat tgggtcgttg
     2821 agaatcacgg tgtctccaat gccgaacggc ggctcacgat cgacaacgtg acgaaggtat
     2881 cgaccgccaa ggtgcttgaa aacgtcagga tcgcggatgt gtggtacgac tcggacagcc
     2941 ggtcgtacca cgctctgggc gtgatgaatc gctctcaagc cgaagccgca ttgatggaga
     3001 aggtgtcggc gtcggatcgc gcgatcgacg cggacgtcac cgagtcacgg caaacgaccg
     3061 acaaacttgc caaggttcgt gctctgcggc gggccggcag aaatctcgtg ttgcgcgagg
     3121 tctacaacac ggatttacgg gtgatccgtc ccagcgggca agggactcca tccgtctatc
     3181 aggtaaacga attgtccggc gagttggagc aatttcttgc tacaaatctg gtgattgcgg
     3241 tacaagtgtc cggcgaccat gcggaaccgg ttggacgtgc tctggaggaa gggctcatcc
     3301 gggaaggact tcaggtcacg acaaagccgg aaggtgaggg gactggctcc cctgaagtga
     3361 tcgttcgagg cactgtccgt ctgttccccg tcgaggtgcg ggacccgaat ttcaagtacg
     3421 ttcggtggtg tagtgatttc gaggtcgtgg agccggggac ggggcgtatc cttggggcgg
     3481 tggcgcaggg tggaagggaa ggacatttga cggagcgtga agcaacggcc aaaactttgc
     3541 gtgtgatgca gcaagagctg tcgtcggatg ttgcaaaggc gattgccgct catatcttcg
     3601 gcgaagtttc gctgcctgag acggccacga tgccggctgg ttgtccacga gaagaatcag
     3661 gagtgaagcg gtagcggtaa tgaacgttct ttcatgagga ggcctttatc gtgaaaaaga
     3721 cagacgaatt acccgtcaac cgatctccac gcagcagggg gcggggactg accaagtcag
     3781 gacaaaaggt atcaagccgt ttggccaagc gccgattgaa cgaccgactg aaggaagaca
     3841 catcgtcttt caatcgaggc aatctcgccg acaccttgcg aaaagaaggg tatgaagagg
     3901 tgccgctcga tgagctgcaa gataggctct ccaaactcca agggccgctc gcggaaatca
     3961 ttctgaaggg gagggtgtaa ggggatgcca tactactatt tcgactcaac cgcactcatt
     4021 aagcgttaca gcatggagcg aggcacgagg attgtgaata agctgatggt gaagcgtggg
     4081 aaggtcgcga tcgtcccgac atggaccgtg acggacttct actcgattct gtgcgcacgc
     4141 gctcaagagg ggcagattac gcgggatgat tgctactcgg ttctctataa gttcgaaatc
     4201 gaatccaagg agggcctcta ccaatttatc gcgccgagga tggaaaccta cctggccacg
     4261 aaagaattgg tcttggagta tccgtccctg cgatcgcctc aggtgatgca tctggcattg
     4321 gcattggagc tgaaaccgtt gcgattgacc gtcgtcagcg cggataccca gctgttggcg
     4381 gcatcaaaaa cggcaggatt gcatatcatt aatcctgcag aagattaacg tttcggatga
     4441 ggacgatcat gtggcttgga tgttgactga tcgaccaggc gatgtctctt ccgaggcctg
     4501 tccgagcaat tccatgacgg catgattcca accggcaggg ccgatgcccg gcgcgcggat
     4561 taggttcggc aggttgatgt cagggtcgta cgagccgtcg ggtttttgca ccaacaccgg
     4621 acgatcgacc gtcaacagga gagg
//
LOCUS       DATMTI010000847         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_116027, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000847 DATMTI010000000
VERSION     DATMTI010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116027"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VN039_13415"
     CDS             complement(<1..581)
                     /locus_tag="VN039_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV46998.1"
                     /translation="MITLRDSDTILIADDDPVIRRNLHVLMESEGYHVVEAADGLEAV
                     KALEALSITLVLLDLKMPVCTGLDVLRDHQDRLGDTPVIVITALGGSSAAIEAMKLGA
                     YDYITKPFDLDEVLFTVRRALEQTTLVAQVRALTSHGDSADSSHDELIGQSPAMLAVF
                     KTIGKVAATHEPVLVLGESGTGKELVANAIHRNS"
     gene            complement(586..>1736)
                     /locus_tag="VN039_13420"
     CDS             complement(586..>1736)
                     /locus_tag="VN039_13420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV46999.1"
                     /translation="QAFASVAHQVLTNFPGVEGGFYLRDANRFSGYSFPTAGAMHPSE
                     LSRTDPPPLEAPLIRQQAQQSLSEQIPLFTVRDVGPSRVMIVTEAIGSERPAPAATWG
                     MIRLTGPEQLKSQLHRYEVSIGLALAGLTLALVLTFTLSQSLARQRSAQERLKEELRR
                     SEQLASLGKMLAGVAHEIRNPLAGIRSTIQLWQRFPDQGHIKDSVDAVLQATERLNEI
                     LTRLLHFTRIEHVERRPIQMNDLVAETFKLLEAQGGVQGVRLELNLERDLPPVLGSPT
                     ALRQALLNLATNALQAMPNGGRLHCRTGEQKQTRAVEITITDTGSGVSAKDQKHLFEP
                     FFTTRPEGTGLGLVLCREIVLQHGGMIELDTRSNPGATFRVVLPAAER"
BASE COUNT          372 a          490 c          480 g          394 t
ORIGIN      
        1 gaattgcgat gaatggcatt tgccactagt tctttgccgg tgccgctttc accgaggact
       61 aataccggtt catgggttgc cgccactttt ccgatggttt taaacacggc gagcatcgca
      121 ggactttgtc cgatcagctc atcgtgagat gagtctgccg aatcgccgtg gctcgtcaat
      181 gcccgtactt gcgccactaa agtggtctgt tccagcgccc gtcgaacagt gaagagcacc
      241 tcgtcaagat cgaacggctt ggtgatatag tcataggctc cgagcttcat cgcttcgatg
      301 gccgcactac ttcctcctaa ggcggtgatg acaatgaccg gcgtatcccc caaccgatcc
      361 tgatgatcgc gcaagacatc caagcctgtg cacaccggca tcttgagatc gagcaggacc
      421 agggtaatgg agagagcctc cagtgctttg acggcttcca ggccgtccgc tgcttccacg
      481 acgtgatatc cctccgattc catgaggaca tgaaggtttc tgcgaatgac cgggtcgtcg
      541 tctgcaatga ggatggtgtc tgaatcccgt aatgtgatca tggcactagc gctcagctgc
      601 cggaagaaca actcggaacg tcgcgcccgg atttgatcgc gtgtcaagtt caatcatgcc
      661 gccgtgttga agcacgatct cgcgacacaa taccagtccc aaacccgttc cttctggacg
      721 agtggtaaaa aatggttcaa acagatgctt ctgatcttta gccgaaacgc cggagccggt
      781 gtctgtgatg gtgatctcta cagcccgtgt ctgtttttgt tcgcccgtac ggcaatggag
      841 gcggcctccg ttcggcatgg cctggagcgc attggtggcg aggttcaaca aggcttggcg
      901 caacgcggtc ggagatccca gtaccggcgg caagtcccgc tccagattca gctcaagcct
      961 cacgccttga acaccacctt gtgcctccag cagtttgaac gtctctgcca ccaagtcgtt
     1021 catctggatc ggccgccgtt ccacgtgttc gatcctcgtg aaatgcaaga ggcgagtaag
     1081 gatctcgttg agccgctctg ttgcctgcaa gaccgcatca acggaatcct tgatatgacc
     1141 ctggtcagga aaccgttgcc agagttgaat cgtcgagcgg atgcccgcca aggggtttcg
     1201 tatctcatgc gccacgccgg cgagcatttt cccgagcgac gccagctgct cagatcgtcg
     1261 taattcttcc ttgagtcttt cctgagcgga tcgttgtcgc gccagcgatt gactgagcgt
     1321 gaacgtcaga accaaagcca gtgtcagacc cgctaacgca agaccgatag acacttcata
     1381 tcgatgaagt tggcttttca gttgctccgg tcctgtgaga cgaatcattc cccaggtcgc
     1441 agcgggcgcg ggacgctcgg agccgattgc ctccgtgacg atcatgacac gactggggcc
     1501 cacgtcccgt acggtgaaga gcgggatttg ttccgagagg ctttgttgag cttgttggcg
     1561 aatcaacggc gcttccaaag gcggcggatc ggttctagac aattctgaag gatgcatggc
     1621 gccggcggta ggaaaactat atccactaaa ccgattcgca tcgcgcaaat aaaacccccc
     1681 ttctacaccg gggaaattcg tgaggacttg atgtgcgacc gaggcaaacg cttgat
//
LOCUS       DATMTI010000848         5279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_116041, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000848 DATMTI010000000
VERSION     DATMTI010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116041"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..578)
                     /locus_tag="VN039_13425"
     CDS             complement(261..578)
                     /locus_tag="VN039_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47000.1"
                     /translation="MSIKSLPSHENHESSLAACLLIGVPVLLAAMALCAWIAWATHGF
                     SSELAPNGWFSHGIVYLFGIVFYGGAEVVFGGAVLLILGVIATVLGRSAIEGWRRVRQ
                     HRK"
     gene            complement(700..1008)
                     /locus_tag="VN039_13430"
     CDS             complement(700..1008)
                     /locus_tag="VN039_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47001.1"
                     /translation="MPSHPSSRGKNLESSIELNLLAGILVLCALLLALFADGGAISRR
                     TPEFSPRLMINQWWLEFVKLIRMIFALTFAGAISVGSFPPTVALGKRFRRVWWWLHHR
                     "
     gene            complement(1280..2173)
                     /locus_tag="VN039_13435"
     CDS             complement(1280..2173)
                     /locus_tag="VN039_13435"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HWV47002.1"
                     /translation="MGNEGTLQIIALSDHTGDKLRKLKEARATRDTDRLHTWKVALRE
                     YQAARAARYASVGHAWRNRRLAAWGLAVLRVMVHGLFTRPPSRPCQVAVGDQEQIWAA
                     GHEGEGKVQSRLVAQLGPDWIALAGYRNGKGEADLLVVGPPGVLGIEVKHIAGVVHVD
                     GESWTRDRYDRYGNCVGRNEPIRDRGGRSPAGQINEVLKDLTAFLAKRHQRPSILGAV
                     VLTHPTARLGTVRGQRVNFVGMASQLNIYGLFAACTAPALPPIDVKGIVALITKDHVW
                     HAQRRSGQGGDGPRSRSGTLA"
     gene            complement(2332..2634)
                     /locus_tag="VN039_13440"
     CDS             complement(2332..2634)
                     /locus_tag="VN039_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47003.1"
                     /translation="MSNRRATSNSMAAELLKALDWVHRTGWPTRGMDTITAMRNFDAN
                     KACSGPFTEFEGLALSLWAAFSDRYEDEIAEHLTRIFLVSLESRLCSGDNEEMHAI"
     gene            3402..3599
                     /locus_tag="VN039_13445"
     CDS             3402..3599
                     /locus_tag="VN039_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47004.1"
                     /translation="MNETIQPQLELIEGGRDVLDRLVLKLVIKGGHKDIGTTIEALKP
                     RGKLTLVEPRDPPLPVQRTRG"
     gene            4686..4985
                     /locus_tag="VN039_13450"
     CDS             4686..4985
                     /locus_tag="VN039_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47005.1"
                     /translation="MNQAITNPYPGVYKTAYLAELGVTIEEFARNPEAALTRVGQYDA
                     CALIQAGLRPLLPMQVRLRQAMEQQWADEGTPVVRLAPPRPMLERHHQPRATLVA"
BASE COUNT         1229 a         1584 c         1436 g         1030 t
ORIGIN      
        1 gtcggctcgg gcgagagttt ctcaacagcc tgctaagggt gatgaccgat ttgccgagat
       61 attccgcggc ttctttttcg gtgtataggc gagggaggga gggggtagca tccatcacga
      121 tctccacttg aggtgcgaag actgtggtac atatgcttgg acatttggtg ctcaaaacag
      181 gcaattgaat ggactgtatg tcgcccatgt agaccgttta acctctggag cacatccggt
      241 tccaacatgt ggagggggca tcactttcga tgttgacgta cccgccgcca accctcgata
      301 gcgctcctcc cgagcaccgt ggcaatgaca ccaaggatca agagcaccgc cccgccgaac
      361 acaacctcgg caccgccata gaaaacgatg ccaaatagat aaacgatacc gtgactaaac
      421 cagccgtttg gtgccaactc ggatgagaac ccgtgggtag cccatgcaat ccaggcacac
      481 aatgccatgg ccgcaagtag gacgggcaca ccgatcagca agcaagcggc cagggacgac
      541 tcgtgatttt catggctggg cagagatttg atcgacatgg cgggcttccc tagactgcaa
      601 aggcgtgtct tggcgagagg aggtttatct gaaagaggta caaatcgaag cacccgaggg
      661 atgacagtat cgcgcgaggc agcgtgcagc ctgtggcgcc taacgatgat gaagccacca
      721 ccacaccctg cggaaacgtt tgcccagtgc cactgtaggc gggaaacttc cgacggatat
      781 cgctccggca aacgtcaacg cgaaaatcat gcggatcagt ttcacgaact ccagccacca
      841 ttgattaatc atgagtctcg gtgagaattc aggcgtccgt ctgctgattg cccccccatc
      901 tgcgaaaagt gcgagcagca gcgcgcaaag gacaaggatg ccagccagca ggtttaactc
      961 gatggatgac tccaggttct ttcctctgga tgaaggatgg ctcggcatgg tcggctcttt
     1021 cgctgcgtgc ttgttctcac aagaacacaa acctcaaata gcgcaacccc tatgcaagag
     1081 acgccatgaa cgccccatcg cttcgggtgt tgaacgcatc tgcgcacctt gcagggattg
     1141 acggattcgg gagcggctcc acctctcgcg accggcaaca acaaacccgc acgccagcct
     1201 gggggaattg tcgaatgcaa cataggggat cgcgcgcact gggagggaga cgtcgcctcc
     1261 accgccaagc cgaccgcttt catgccaagg tacccgagcg tgagcgaggc ccatctccac
     1321 cttgaccact gcgacgctgc gcatgccaga catggtcctt ggtgatcagc gccacgatcc
     1381 ccttgacatc tatcggcggc aacgccggcg cggtacaggc agcaaaaagt ccgtagatat
     1441 tgagctgtga cgccatacca acgaaattga ctcgttgccc tctcacggtg ccaaggcgtg
     1501 ctgtgggatg cgtcagcacg acggccccca gtatggacgg ccgctggtga cgcttggcaa
     1561 gaaaagcggt gagatccttc aggacttcgt ttatctgccc tgccgggcta cgccctcctc
     1621 gatcacgaat tggttcattc cggcccacac agttgccata ccggtcatag cgatcacgtg
     1681 tccacgactc accatccacg tgcaccacgc ccgcgatgtg cttgacctca atgcccaaga
     1741 caccaggcgg tcccaccacc aacagatcgg cctcgccctt gccattcctg tagccggcca
     1801 aggcaatcca gtccggcccc aactgcgcca caagtcgcga ttgaacctta ccctcaccct
     1861 cgtggcccgc tgcccagatc tgttcctggt cgccaacggc cacttggcac ggacgcgaag
     1921 gcggccgcgt gaacagcccg tgcaccatga cacgcagaac tgccagaccc caagccgcta
     1981 gtcgtcgatt gcgccaggca tgtccgaccg aggcataacg tgccgcacgg gcagcttggt
     2041 attcccgtag cgccactttc catgtgtgaa gccggtcggt atcgcgcgtc gcgcgggcct
     2101 ctttcaactt gcgaagcttg tcacccgtgt gatccgaaag cgcgatgatc tgcagggtac
     2161 cttcgttgcc cataggaggt gtcctccaaa gttcgtgatg ccgtcttgaa tatcccggtg
     2221 atcacacaga tcacgaattg cgcaagcgat tgcacgcccc gtggaacttc cgtgtccatg
     2281 cacgactacg gcataacgtc acaccatcgg atgacacggg tcgccatagg ctcagatcgc
     2341 atgcatctct tcgttgtcgc cggagcacag gcgactttcc agcgagacga gaaaaatcct
     2401 ggtcaagtgc tctgcaatct catcttcgta ccggtcgctg aaagcggccc aaagggacag
     2461 tgcaaggcct tcgaattccg tgaacgggcc gctacaggct ttatttgcat caaaattccg
     2521 catcgccgtg atggtatcca tccctcgtgt gggccatcca gtgcgatgca cccaatccag
     2581 cgccttgagc aactcggcgg ccatgctgtt tgatgttgcg cgacggttag acatgacggc
     2641 tccatgtgta gcggaatgat gctacccaag cacagtggtt ccatcgcgca agtgctcctt
     2701 taacatggtg tcttccaatg gtctcttccg cgtgtcagcg tccagtccgg acgctatcca
     2761 tcttttctga tcgacgctgc cgccttgcat atcgacgctt gacttccgac atggcgtgcg
     2821 cgaaagtcgc agtcgtcatc gttgaaggtt cagcctccga tgcgacacat ctgccgaccg
     2881 atggttttcc cagagttgaa aatttggatc gtacgatcca aaaaccgttg ggacgcgcga
     2941 tttgccttcg ggcgtacgct cgaattattc gtccgtcgtc cgcctcttac gtagcttcac
     3001 catgattgcc taccgcgcat cggcgtccag ttgatagcac tgctcctcca ttcaatcagg
     3061 cgctgtgggt gtgatgacat catcatgagt cgagctgtcg ccgttcgact gcttccttgt
     3121 acacagcccc agcataacca gccagccatc attcagcagc cctttcggct gatcggcata
     3181 caaacgtcat gggaaaacga ggcagtgccc gtcttacgct acgactgatc gagatccgta
     3241 ttgcctgcga taacctcgaa cccatgatca gttcatgcta gtcgcagcct tcggcatggc
     3301 tccgtcaaac gacaaatcgt gaatactcag ccctcgcaaa ggccaacgac agggctggcg
     3361 caaagcgtgg aggcgcgccg tccattcgga accaggagtc catgaacgaa accatccaac
     3421 cgcaattgga actcatcgag ggcggccgcg atgtactgga tcgtcttgtg cttaagttgg
     3481 tgatcaaagg tggccataaa gacatcggca ccaccattga ggcactcaag ccacgcggta
     3541 aactcacact ggtcgaaccc cgggacccgc cattgcctgt acaaagaacc cggggatgac
     3601 ctgaagcgcg aacgcccgtc atgcatacga tgtcagcggg ccagcctttc accgtcaaca
     3661 atcgacctcg actttttgtt gaggcaccgc atccggatat ccgggattaa aatcttctga
     3721 ctgacgggca ccaggcggaa actggcagcc aactcattgc actcatattg cgaaacgcgg
     3781 atgttcggcc gtcagtatcg tcatgcactc agcgttgccg tcgcatgagg acgtccagta
     3841 tcgacagggc tagacagcgg cacgtccgta acaaggtttt atccggctgc cgtccctctt
     3901 ctcctaccga cctcgtctgc gatggcactc catgggcgat tgaatcgagc acaaacggat
     3961 cgaataggcc cgactaggca tctctgattg gagtggacgg aaaagacacg tctaggcact
     4021 acaaggcatt agggcgacga caggctaggt acggctaggc acaagtaggc agtgatagcc
     4081 tgaataggcg cgagaacgca tcaatagaca gaaaaaggca cgcaaaggca tgacgccaat
     4141 catcgataat tatgattagg ctcagcgagg catggaatgg catggaatgg catgacatga
     4201 catgacatga catgacatga catgacatga catgacatga caaggcatgg aagagtataa
     4261 gcgcatgctt cgcgtatcac gatgcagttt agtactcgcc gtcagggaac cctactacag
     4321 aacgccttgc ggtcccggta gaccatcccg gccacagcca cgattcggct acgtacacaa
     4381 ggcatcccgt ccttctggac gtctgttcgt cggccacctg cgaccacgcg taacgtcgtc
     4441 aacacgacaa gcgttggcca caaggccatg ccgcccaact cacggacgta cgaacaaaac
     4501 agataggtgc acaagtgacg cgcgccagtc acttgtgcac ctatctatgt agataatggg
     4561 tgcacaagtg acaccacata tggaagaggt gcccttgatg gaagggggct ggaccatggg
     4621 gttgcgcgta cgacgaacca tggtatcgag gtatccagac cacacgaacg gacatggagt
     4681 ccaccatgaa tcaggcaatc accaatccct acccaggcgt ctacaagacc gcctatctgg
     4741 ctgaactggg cgtcacgatc gaagagttcg ctcgcaaccc cgaagctgca ctgactcgcg
     4801 ttggccagta cgacgcttgc gcactgatcc aggccggcct gcgcccgctg ctgcctatgc
     4861 aggttcggct acgccaagcg atggagcaac agtgggccga cgaaggcacc ccggttgtgc
     4921 gcttggcccc gccaagaccg atgctcgaac gacaccacca gcctagggcg acgctcgtcg
     4981 catgagccag ttgagtaaac gtacggtcga tgacatcaag gaatgactcg gcaccctggc
     5041 atccagcaag ccgagatttc agaggcgcca ggagcaggat cgcgccatct acgcgatcgc
     5101 gtgggcactc gaagcggaag acgatgatgc cgtacgcccg atcgccattg agggaccaac
     5161 gagtattggc aagagcatcg tcgacctgat tgtcggcatg gtggccgccc accgtcacgg
     5221 aaagacgctg atcgtaattg cggctaccat cgcgctgcag cagcggctcg ctgccgacc
//
LOCUS       DATMTI010000849         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_329142, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000849 DATMTI010000000
VERSION     DATMTI010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329142"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1542)
                     /locus_tag="VN039_13455"
     CDS             complement(178..1542)
                     /locus_tag="VN039_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV47006.1"
                     /translation="MNSKILIVDDDPDIVMMLEDRLQASGYETVAATEGQQALDQIVH
                     EFPQLILLDLTLPKVSGLDVLKRLSQMKPSDNIPVIVMTAHASIEAAVEAMKEGAYDF
                     LTKPLDGDHLLIVIRKALERDALRRQVAYLRSEVDGRYASIVGNSPSVRSVVEAAQRA
                     AKSDASVLLLGESGTGKELFARSIHQWSPRCAMPLIVINCVALTETLLENELFGHERG
                     AFTGADRQQKGKLEMADGGTVFLDEIGDMSLPLQAKLLRVLQDREFQRVGGSKTVTVN
                     IRIIAATNKDLRQAVRAGQFREDLYFRLNVVTLTLPPLRERSGDVAALAQFFLDRHTR
                     DAKRPGMTLNTAAFDTLIRYPWPGNIRELDNVIARAVVLSPKDIIEPEMLALLADDTN
                     LRRRDDAPLPYLNLPYHESMEQHSRYIIARAMEHAEGNQTKAAESLRLQRTYLARLLK
                     QQKE"
     gene            complement(1643..2965)
                     /locus_tag="VN039_13460"
     CDS             complement(1643..2965)
                     /locus_tag="VN039_13460"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HWV47007.1"
                     /translation="MSQIFPFQGMLYDQTLVGSIKDVVAPPYDIIDAEGQKRLHDRHP
                     HNIIRMELGLDQTGDSPAHNRYTRAAATLQTWLNEGILKRDAQPAVYFHTIEYTPPYS
                     ALDAPKKMLRGFLAQAKLETLDSGHIYPHENTRAAAKTDRLNLIEACRSNFSPIWSLY
                     SDPLGAIIQLLETAVKGKPARYDFQDDAGCRECLWGVTDETVIQQITAAMQTKPLFIA
                     DGHHRYETALNYQKLRRQQAGPQTGLQPYDSVLMLLTPLEDPGLTVLPTHRVATTPLP
                     SSDRIRTVLGDAFEFREFPFSASTQQQARSQFLTSLRTEGKNTPVFGLARQSDDRYVT
                     LTLKPAHRPSPQASPRAKLDVSLLQQLIVTRLCPTQQEQEAILYTKDDHEALDWVTRG
                     SGTGAFLLNATKVSEVQAVAAAGERMPHKSTYFYPKPLTGLVINVMTG"
     gene            complement(3092..>3386)
                     /locus_tag="VN039_13465"
     CDS             complement(3092..>3386)
                     /locus_tag="VN039_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWV47008.1"
                     /translation="GQDLRQGKATLPLLHLLQHCSEQDRQMIKDRMETRTLSTADLER
                     ILLLMADFGSITYAMDRASAYIAAAKHELERFDDSTARRALSVTADYMITRDR"
BASE COUNT          676 a          925 c         1016 g          769 t
ORIGIN      
        1 attggtcgga cgtcggtacg gccgaccgct atacgcaggc ggaacaggat gtccgagcgg
       61 gactgattcg tctggaagac cgacagtctt tcgaaccgcg cgttccgctc aaataagaag
      121 caggtgatct cgaccaccgc gctccaccga tggccggccg gtcagctccc atcatgtcta
      181 ttccttttgc tgtttgagta accgagccag gtaggtccgc tggagcctga gcgattcggc
      241 ggctttcgtc tgattgcctt ccgcatgctc catcgcgcgc gcgatgatat agcggctatg
      301 ttgctccatc gattcatgat agggcaggtt cagatacggc agtggtgcgt cgtctcttcg
      361 acggaggttg gtgtcatctg ccaacagtgc caacatctcc ggctcgatga tgtctttcgg
      421 gctcaagacg acggcgcgcg cgatcacatt atccaattct cgaatgtttc ccggccaggg
      481 gtagcggatc aaggtgtcga atgcggctgt attcagagtc atgcctggtc gtttcgcatc
      541 tctcgtatgt cgatccaaaa agaactgggc aagggctgcg acatcccctg accgttcgcg
      601 aagcggcggc agggtcaagg tcacgacgtt gagtcgaaaa tagagatctt cgcgaaactg
      661 gcctgccctc acagcttgcc gaagatcctt gttcgtggcc gcaatgatgc gaatgttcac
      721 cgtgacggtc ttggagccgc cgacgcgctg aaactcccgg tcttgcaaga cccgtaaaag
      781 tttagcctgc aatggaaggg acatatctcc gatctcgtca aggaagacgg tcccgccgtc
      841 ggccatttcc agcttgccct tctgctgccg gtcggctccg gtgaacgccc cacgctcatg
      901 tccgaatagt tcgttctcca ggagcgtctc cgtcaaggcg acgcagttga tgacgatgag
      961 cggcatggcg caccgaggac tccactgatg gatcgaccga gcgaagagtt ccttgccggt
     1021 cccgctttcg ccgaggagca acacgctcgc atcggatttt gcggcccgtt gtgccgcttc
     1081 cacgacggac cttacggagg ggctattgcc gacgatggag gcgtagcggc cgtcgacctc
     1141 ggaacgaagg taggcgacct ggcgtcgcag cgcatcccgc tccaaggctt tgcgaatgac
     1201 gatcaagaga tggtctccgt cgaggggttt cgtcaggaag tcgtacgctc cttccttcat
     1261 ggcctccacc gcggcttcaa tggatgcatg cgcggtcatg acgatgacgg gaatattgtc
     1321 cgacggcttc atctgggaca gacgcttgag gacatcaagc cccgacactt tcggcaatgt
     1381 gaggtccagt aggataagct gcggaaattc gtggacgatt tggtcaagcg cctgctgccc
     1441 ctcggtggcc gcgacggtct cgtacccgga ggcttggagc cggtcctcga gcatcatgac
     1501 gatgtcggga tcatcgtcga caataagaat tttggaattc atcggaataa ctctctgcct
     1561 ctggtcatcg cggccgttat ggacggatcg gccagagagt cgtatcgcgg ttgatgagcg
     1621 gcgatgtgta aaacgtctcc acctaaccgg tcatcacatt gatgacgagc cccgtcaacg
     1681 gcttcgggta aaaatacgtt gatttatgcg gcatccgttc tccggcagcc gcgacggctt
     1741 gaacctcact gaccttcgtg gcgttgagca ggaatgcgcc cgtccctgat cctctagtca
     1801 cccaatccaa ggcttcgtgg tcatctttgg tgtagagaat ggcttcttgt tcctgctgcg
     1861 tcggacagag cctggtcacg atcagttgtt gcagcaggga gacgtcgagt ttggctcgag
     1921 gggatgcctg aggggaaggt cgatgggccg gcttcagcgt gagcgtgacg tatcggtcgt
     1981 cgctttgccg cgccagtccg aacaccgggg tattcttccc ttctgtccgc aagctcgtca
     2041 ggaactgtga ccgcgcttgc tgttgtgtgg atgcactgaa ggggaattct cgaaactcga
     2101 acgcgtctcc cagtaccgtc ctgatccggt ccgaagaagg caagggggtg gtcgccactc
     2161 gatgtgtcgg caataccgtc aatcctggat cttcaagggg ggtgagcagc atgagcaccg
     2221 agtcgtaagg ttgcaacccg gtttgagggc ccgcctgctg tcgccggagt ttttgataat
     2281 tcaacgccgt ttcatagcga tggtgaccgt ccgcgatgaa aagcggcttg gtctgcatcg
     2341 cagcggtgat ttgttgaatg acagtctcat cggtgacgcc ccacaggcat tcgcgacagc
     2401 ccgcatcatc ttgaaagtcg taacgcgccg gcttgccttt taccgcagtt tccagaagtt
     2461 ggatgatggc accgagtgga tcggagtaga gagaccaaat cgggctaaag tttgagcgac
     2521 aggcttcgat caaattcagg cgatcagtct tcgccgcggc gcgtgtattt tcatgaggat
     2581 aaatatgccc ggagtcgagc gtctccagct ttgcctgcgc caagaatccc cgaagcatct
     2641 tcttgggagc gtcgagggct gaatagggag gagtgtattc aatcgtgtga aagtagacgg
     2701 ccggctgtgc atcacgcttg agaatacctt cattgagcca ggtctgcaac gtggctgccg
     2761 cgcgggtata ccgattgtgg gcagggctgt caccggtctg gtcaagtccc aattccattc
     2821 ggataatatt gtggggatgg cgatcgtgaa gtcgtttctg tccctccgcg tcaataatgt
     2881 cgtatggcgg agcgaccacg tccttgatgg agcccacgag cgtttggtcg tacagcatgc
     2941 cctgaaacgg aaagatctgt gacatgaaca accccttggc aatgtcgtga cagcgacgac
     3001 ggtttgatgg ttacaggaga gagtgggatc ctttcgccct acgccgtgcc attcccatgg
     3061 cccagcgccc ttccagctgg caggaacgca gctaccggtc gcgggtaatc atataatcag
     3121 cggtgaccga cagcgcgcgc cgcgccgtac tgtcgtcgaa gcgttcgagc tcgtgtttgg
     3181 cggcggcaat gtaggcgctt gcgcgatcca tagcataggt aatcgaaccg aagtcggcca
     3241 tcaacaataa gattcgttca agatcggcgg tgctgagtgt ccgagtttcc attcgatcct
     3301 tgatcatttg gcgatcctgt tccgagcaat gctgtaacag atgcaacagg ggtagcgtcg
     3361 cttttccttg ccggagatcc tggccg
//
LOCUS       DATMTI010000850         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_245385, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000850 DATMTI010000000
VERSION     DATMTI010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245385"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VN039_13470"
     CDS             complement(<1..182)
                     /locus_tag="VN039_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47009.1"
                     /translation="MKRRGADQGDIADRIGGGPGHVARASLTRSVESSTITNPLQSFI
                     ASSFLVGKPDDNRLWTL"
     gene            complement(781..1428)
                     /locus_tag="VN039_13475"
     CDS             complement(781..1428)
                     /locus_tag="VN039_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47010.1"
                     /translation="MSRGAVIGGLVVLFVAGVLCGVAGISFYHTYEREQRAERGASAQ
                     HDRIMTRLTQELALTAEQRTDIEPIVTEAHVAILELRFAHQAEIEAILMNGMAALKAK
                     LSPEQQTRLDAIYARLQRRWERSRDYLESTKKRLTWLVPFYPPSDGERLMRTHLGMGT
                     LDKMNAQAHWRDEQGLGESFLSPPPMMDTAQMLCRRDGHAGNLTSNYFRDWLTLC"
     gene            complement(1455..1772)
                     /locus_tag="VN039_13480"
     CDS             complement(1455..1772)
                     /locus_tag="VN039_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47011.1"
                     /translation="MSTVDDKRLRKLEEALTEAHRVRHTPPWQEGWAEGVMQEVRRLA
                     RRQPQISGHGEVVHLIWRAAGFAAVLSILLLVSFLMQSTMTVSEEGGLMAEDVEVGSL
                     FFE"
     gene            complement(1769..2413)
                     /locus_tag="VN039_13485"
     CDS             complement(1769..2413)
                     /locus_tag="VN039_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWV47012.1"
                     /translation="MAEHYYPTGGMSLEALPGDRMAQQEWVDGDLVQRVVAGETERFE
                     DLMLRHRDHVRRIVGGHVPPDRVAEVAHEVFVKAYIGLGTYRFEEPFPHWLATIAVRT
                     CYDFWRTQQAADLPVSTLTAEHQRWIDHVMAAQSDDGFAEQARRQEASEVLQWALARL
                     SPENRLVVTLVHLDGYSVREAAALLGWSVINVKVRAHRARQLLRTILREEVAKP"
     gene            2703..2990
                     /locus_tag="VN039_13490"
     CDS             2703..2990
                     /locus_tag="VN039_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47013.1"
                     /translation="MASMNGLFLLVFLTGCRSAQDGYLTQATGHATAVELEQVLGHPS
                     HEQVLETGQRLWLYRREGSGTGGQDFAPFCQDLWLTFDRNGVLRTWEKQRC"
BASE COUNT          662 a          968 c          889 g          703 t
ORIGIN      
        1 agcgtccaga gtctgttgtc atccggtttt cctacaagaa aggaagatgc aatgaagctc
       61 tggagagggt tagtaatagt gctgctctcc acgctcctcg tgagcgaagc acgtgcaacg
      121 tgaccaggtc ccccgccgat gcgatcggca atgtcgccct gatcggcgcc gcgacgcttc
      181 aaggtggctc attgaacggt gaggatttcg cggtcatctt caccggaaca ataacgccgc
      241 ccccggcgtg agattcttcg accaaacggt ctagtttcca cgctgccatc gtgtcgccgc
      301 gctccagtta tttaaggcaa cgttgcaggg caggcagcta aagattgcac cgcttctgcc
      361 ttcatcaacg aagttttttt gcagcctgcc cctttgagcg cagcaagttg caagtcggtg
      421 tttggtcgtc tggccttaaa aaccgcctta gaaacgatat cgagggaagc cgccggtttg
      481 agcgggtaga attcgcggaa agccacctgt caggctgatg ccgtgcccat tcacctacgg
      541 tttttcatgc tcgtccctga gctgcactgt gacgccgtgc tcttcaaaaa tgcctctgag
      601 catgtccaca cattgaattt ttacttcccg atcggtttat tctgaccagg tccggcgccg
      661 gcgatggatt gatgcggcct tcctatcatg gccgttgacg cggagctcaa caacaggcag
      721 ttcgctccgt gagctgcctc cagagagctc tcggtcatcg tcgcgatgac tgagcggtaa
      781 tcagcaaaga gtgagccagt cccgaaaata gttcgacgtg agattgccag catgaccatc
      841 tcttcggcac aacatttggg ctgtatccat catcggtggt ggggataaga atgactcgcc
      901 gaggccttgc tcatcacgcc aatgtgcttg cgcgttcatc ttatcaagag tgcccatgcc
      961 gagatgcgtg cgcatcagtc gttcaccgtc actcggcgga tagaatggca cgagccaggt
     1021 caagcgtttc ttcgtggatt caagatagtc acgagatctt tcccagcgcc gttgtaaccg
     1081 tgcgtatatt gcatcgagcc gcgtctgttg ctcgggggat aatttcgctt tgagcgccgc
     1141 catgccgttc atcaagatgg cctcgatctc ggcctgatgc gcaaatcgta attccaggat
     1201 ggccacatgg gcttccgtca caatcggttc aatgtccgtt cgctgctcgg ccgtcaacgc
     1261 caactcctgc gtgaggcgtg tcatgatccg gtcatgctgc gcggatgccc cacgttccgc
     1321 gcgttgttca cgttcgtacg tgtgatagaa actgatgccc gcaacgccgc acagcacgcc
     1381 ggcgacaaag aggacgacca gccctccgat gactgcacct cgtgacatcg tacctgcctc
     1441 cttttcctgc gcatctactc gaaaaacaat gagcccacct cgacgtcctc cgccatgaga
     1501 ccgccttctt cgctcacggt catagtagat tgcatcagga acgacaccag cagaaggatg
     1561 gaaagaaccg ccgcgaaccc ggccgcccgc catatcaggt gcacgacctc accgtgtccg
     1621 ctgatctgtg gctgccgtct cgccagacga cggacctcct gcatcacgcc ctcggcccat
     1681 ccctcttgcc acggcggtgt gtgtcgtacc cggtgggctt cagtgagcgc ctcttccaac
     1741 tttcgcagcc gcttgtcgtc gacagtgctc atggtttggc gacctcctct ctgaggatcg
     1801 tgcgcaggag ctgccgggct cgatgcgcgc gcactttgac gttgatcacg ctccacccta
     1861 ataacgcggc cgcctctcgg acggaatacc cgtccagatg caccaaggtc acgaccaaac
     1921 ggttctcagg cgacaggcga gccaatgccc actgtaacac ttcactggct tcttgccggc
     1981 gggcctgttc ggcaaagcca tcatccgact gggccgccat cacgtggtcg atccagcgct
     2041 ggtgctcagc cgtgagcgtg ctcaccggca agtctgccgc ctgttgcgtc cgccaaaagt
     2101 cgtaacaggt gcggacggcg attgtggcca gccaatgggg gaagggttct tcaaaccgat
     2161 aggtcccgag tccgatgtat gccttcacaa agacctcatg cgcgacttcg gccacccggt
     2221 cgggcgggac gtggcctccg actattcgcc tcacatgatc ccgatggcgg agcatcaaat
     2281 cttcgaagcg ctcggtctcc cctgcgacga cccgctgtac caggtcacca tccacccact
     2341 cctgttgagc catcctgtcc cccggcaacg cctcaaggga catgcctcct gtagggtagt
     2401 agtgctcggc catacgcggg cgcgtccccg tgagaagaat gccagctccc tctctctatc
     2461 tcaagacgaa acgacgggtc aaaaggttac aggtctccgc caaatcagaa agaaggatgt
     2521 cactatccgc ttcagccatt cattcagacc cacatcgcgt gtaacctttc cgctctccat
     2581 tccgtcctag aaggagagag ccatcggagg tttcgatggg tcggcggcat ggccccaatg
     2641 gtcacaaggc gcatcacgag acttcgcaaa cgtgttccaa aatccgtcga tggtggtttc
     2701 gcatggcaag tatgaacggc ctgttcctcc ttgtgttcct gacagggtgt cgctcggcgc
     2761 aggacggtta cctcacgcag gccactggtc atgcgacggc ggtggaactc gaacaggtct
     2821 taggacaccc cagtcacgaa caagtcctcg aaacaggaca acggctgtgg ctctatcgcc
     2881 gtgaagggag cgggacaggc ggccaggact tcgcaccgtt ctgtcaagat ctctggctga
     2941 cttttgaccg taacggagtc ttgcgtacgt gggagaaaca acgctgttga gccgaacaga
     3001 acagacggtc gggcatggat gggtctttct acagaaagga gagttcacat gttacaaacg
     3061 cgattgagcc ggtggacagt gaccatgtgt gttgcaggaa taggcctggc cgctgtcttg
     3121 cttgttccga acgagggcta tgcggattca ggtggaggca cttggttccg tcattgggaa
     3181 cagaagaaag attatgccag ccgaacattc cggttgctgc tc
//
LOCUS       DATMTI010000851         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_286120, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000851 DATMTI010000000
VERSION     DATMTI010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286120"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VN039_13495"
     CDS             complement(<1..718)
                     /locus_tag="VN039_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV47014.1"
                     /translation="MNKSKPIRLVIVDDHEVVRIGLCAVLNLTPGMKVVGQGGRVKEA
                     KKLCHHLKPDLVLLDIRLPDGSGVDAAREILAKCPDTRVLFLTSFADDHTVLEAILSG
                     AQGYILKDIASNALIRAIRTVASGQALIDPRLTKHTLEWLSTMYSEPGQSKRSLLSPQ
                     EQRLLPLVAKGLTNKEIAHDLRLSEKTVKNYLANIYSKLNIGRRSQIAAFYAGSFKGS
                     GALYPSRLSESVKADRERTTP"
     gene            complement(711..>2078)
                     /locus_tag="VN039_13500"
     CDS             complement(711..>2078)
                     /locus_tag="VN039_13500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV47015.1"
                     /translation="SYSLLQAAINSVADGLLVIDRQGKVTSVNQRFLHLWNIPQAPAD
                     DRDDEELLAFVVDQLREPEAFLRKVRELYENPKQESFDVLDFKDGRVFERYSRPQILD
                     GEIVGRVWSFRDVTEHRRAEEALRAGEHTLQRFVAEAPVGLCILDENWRAISANKAFC
                     ELTGYEEHEIIGSTYALYTHPEDLSANIVLTDEFFRGIRSGYTYEKRYIRKSGEIIWV
                     SVKATRVELSGHAGPLLLAAVQDVTERKLAMEEREHLSRDLHDNILQALYAVGMQLEA
                     GKLSMGKSPRRSKIHMTQAVDQLNNLMLDVRRFIALLAHRTTAEPDFGQALQQLIASM
                     PGAGQSAPELDITNPVLSFITPKLGEQLLNIVREALSNSSRHARASRRWVRLSLADNT
                     IHLIVGDNGVGFSSKRKRRGGNGLGNMAARAKQISATFTLESAPGKGTTVLVDVPLKK
                     GTVYE"
BASE COUNT          446 a          565 c          580 g          487 t
ORIGIN      
        1 gaggagtcgt cctttcgcgg tcagctttga ccgattcact gagcctcgat gggtacagtg
       61 cacctgaacc cttaaagctc ccggcataga aggcggcaat ctgggatcgg cgcccgatat
      121 tcagttttga atagatgttc gcgagatagt tcttgacggt cttttcgctt aaccgcagat
      181 catgggcgat ctctttattt gtgaggccct tggccaccaa cggcaaaaga cgttgctctt
      241 gtggagaaag aagagagcgt ttggattgcc ccggctcact atacatagta ctcagccatt
      301 ccagtgtatg cttcgtcaag cgaggatcga tgagagcttg acctgaggct acggtccgaa
      361 tggcgcggat cagggcattg gacgcaatgt ctttcaggat gtagccttga gcgccggaaa
      421 gaatagcttc gagtacggta tggtcgtcgg cgaagctcgt taagaacaac acgcgagtgt
      481 cgggacactt ggcaagaatc tcgcgcgcgg catccacccc gctgccgtcc ggcaaccgga
      541 tgtcgagtaa cacgaggtcc ggtttaagat gatgacagag tttcttcgcc tctttcaccc
      601 gtccaccttg tcccacaact ttcatgccgg gtgtcaaatt gaggaccgca cagagtccga
      661 tacgtacgac ttcatgatcg tcaacgatga cgaggcgaat cggtttggac ttattcatac
      721 acagttccct tcttcagcgg aacatcgact aggacggtgg ttcccttccc cggcgcgctc
      781 tccagcgtaa aggtggcgga gatttgcttg gcccgggcgg ccatgttgcc tagtccatta
      841 ccccctcgac gtttgcgctt ggaggaaaat ccgaccccat tgtcgccgac gatcaagtgg
      901 atcgtgttat ccgccagact gagtcgaacc caccgacgag acgcgcgggc atgacgagag
      961 ctattgctga gagcttctcg tacgatgttg agtaactgtt ctcccagctt gggggtgatg
     1021 aaggacagga ccgggtttgt gatgtccagt tccggtgcgg attggccggc gccgggcatc
     1081 gatgcaatga gctgctgcag ggcctgtccg aagtccggct ctgctgtggt gcggtgcgcc
     1141 agcaaggcaa tgaaccgccg aacatcgagc atcagattat tcagctggtc aacggcttga
     1201 gtcatatgga ttttggaccg ccgaggggat ttccccatcg acaacttgcc tgcttccaac
     1261 tgcatgccga ccgcatagag ggcttgcaaa atattgtcgt gcaagtctcg gctcaagtgc
     1321 tcgcgttcct ccatcgccag tttgcgctcg gtaacgtcct gaaccgcggc aaggaggagc
     1381 ggcccggcat ggcctgacaa ctcaacacga gtcgctttga ctgaaaccca aataatttca
     1441 ccggacttac gaatgtatcg cttttcgtag gtatatcccg atcgaatccc ccggaaaaac
     1501 tcgtccgtca atacgatatt ggcggaaaga tcttccgggt gtgtatagag ggcataggtg
     1561 ctgccgataa tttcatgttc ttcatatccc gtcagctcac aaaaggcttt gttggcgctg
     1621 atcgcgcgcc agttttcatc gaggatacat aacccgaccg gggcttcagc cacgaagcgc
     1681 tgaagtgtat gctcgcctgc gcgtaaggct tcctcagcgc gtcggtgttc ggtgacatct
     1741 cggaaactcc agacacgccc gacgatttca ccatcgagaa tctgtggccg agaatagcgc
     1801 tcgaagaccc gtccgtcctt gaagtcaagg acatcgaagc tctcttgttt cggattctcg
     1861 tacagctctc gaaccttcct caaaaatgcc tccggttccc gcagttggtc gacgacaaat
     1921 gccagcagct cctcatcatc ccggtcgtcc gccggtgctt gtggaatgtt ccataggtgc
     1981 aggaaccgct ggttgacact ggtcacctta ccttgtcggt ctatgacgag caggccgtcg
     2041 gcgactgagt tgatggcggc ttgaaggagt gagtaaga
//
LOCUS       DATMTI010000852         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_839492, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000852 DATMTI010000000
VERSION     DATMTI010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839492"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VN039_13505"
     CDS             <1..267
                     /locus_tag="VN039_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47016.1"
                     /translation="EILREFVGEETLNGYPTELFEVTVAGNGQTRQYYRWVTKTERFP
                     LKTVSKQGKWSEEFRRLIFTKQSPLLFELPQRLDPADQSAGTQH"
     gene            287..1072
                     /locus_tag="VN039_13510"
     CDS             287..1072
                     /locus_tag="VN039_13510"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWV47017.1"
                     /translation="MGQKFLRGLWLAGSVSAILMSGLQADAGAYESGAVEDGAVVRGK
                     VTFTGTVPDPKEFELRRSPDREFCSAVSDGNGHRLLKEVTIGPDGGLKDVVVVLEGVE
                     RGKPFTFTDAEVEASLCQFLPFVTVVSDKRRVTVFNRDPVLHDIQGYAYNEAGVDIVL
                     QRPALHESGTTDIVKLVKGRKVFTMQCGMHPYMQNWGYAIDNPYYAITDLNGSFAIGD
                     IPAGTYHLKAWHPILGTQERDITVKPNETISLDWLFDAKRVIE"
     gene            1069..1290
                     /locus_tag="VN039_13515"
     CDS             1069..1290
                     /locus_tag="VN039_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47018.1"
                     /translation="MIKGPHALCLAIVLGMPTVGTLAAQPDSITSSGRVEVVMANEHR
                     KDLEEIRKEFAEAGLTNLHVRRSWQGRRK"
     gene            1321..1521
                     /locus_tag="VN039_13520"
     CDS             1321..1521
                     /locus_tag="VN039_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47019.1"
                     /translation="MRRTWSCTEAWATAAPLIQEAITHCKRVADMGDKVDPGASLNPA
                     TKARAAAKEAMKHLMAMRDNGA"
     gene            1949..2749
                     /locus_tag="VN039_13525"
     CDS             1949..2749
                     /locus_tag="VN039_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47020.1"
                     /translation="MKTRLGRQETQLLAYLQLRKRRTVRTGELTGPLRLNRDQERELF
                     RRLSRGGLIARVRPGLYLVPAHLPLGGVWAPNEILALNTLMEDRNGRYQICGPNTFNR
                     YGFDEQIPVRVYAYNNRISGERTIGSIVFHLIKVADARLGSTEETKTAEGETAVYSSR
                     ARTLVDAVYDWSRFNSLPRGYRWIERELVAKRVTAADLVTLTLRYGDIGTIRRVGVLL
                     EREGVPQPLLTKLEEALSSSTGMIPWIPIAPKRGTVDRRWGVVINDGV"
     gene            2756..3622
                     /locus_tag="VN039_13530"
     CDS             2756..3622
                     /locus_tag="VN039_13530"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HWV47021.1"
                     /translation="MRDLFREVELFREALIMTQQQYGFPERLIEKDYFCSLLLELLNS
                     AHGDLVFKGGTCLAKVYADFYRLSEDLDFVIPMPTDAPRSARSRQATKLKDIIASLPR
                     ECPYVRVIEPLTGANNSTQYNAVMGYTSSIGRGEETIKIEVGLHEPLLLPPLNGEAKT
                     ILLDPVSGQALVPMKVRCISQREAFAEKFRAALSRREVAIRDFFDIDYAVRTLGLQPN
                     DETMVKLVQAKVGVSRNEPVNVSAERLAILRQQVDAQLRPVLRERDFRAFDLDRAFRT
                     VANMADRLTARL"
     gene            complement(3748..>4483)
                     /locus_tag="VN039_13535"
     CDS             complement(3748..>4483)
                     /locus_tag="VN039_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47022.1"
                     /translation="KTMGRTATRSGAVPELRPSGFAVPSRSGNGGYICLMKLLMIREY
                     LMFALVAMSLVPHATPALGAVTDTNEGTGWKLYTNDRFGFSVRYPDDWRLGNPLPDGI
                     GVTLYPPIEQSLVALSGHMNVVSGRSQDGRQTLKEFADAHRRIITDLYDKKQVQVTWQ
                     KDQEATLAGFPAKRLAFTYQDDKRNAVLEVHILSLGRNEGRGVRIKMPADRTDQLMPT
                     ITRMLKSYEAGRDQNAVSPLVPKGKG"
BASE COUNT          973 a         1216 c         1346 g          948 t
ORIGIN      
        1 gaaatcttgc gggagttcgt aggggaggaa acgctgaacg gctatcccac ggagttgttc
       61 gaggtgacag ttgcagggaa cgggcaaact cgccaatact atcgatgggt gaccaagacc
      121 gaacggtttc cactcaagac cgtgagcaag caagggaagt ggtcggaaga gtttcgccga
      181 ctgatcttca ccaagcagtc tccgttgttg ttcgaactcc cgcagcgatt ggatccggcc
      241 gatcaatcgg caggtacgca acattgagcg cgcgaccgga ggatatatgg gacaaaaatt
      301 tctgagagga ctgtggctgg ccggctctgt gtcggctatc ttgatgtcgg gacttcaagc
      361 agatgccggg gcgtatgaat caggggctgt ggaggatggt gcggtcgtgc ggggcaaggt
      421 cacgttcacc ggaaccgtgc cggatccgaa agagttcgag ctgcgtcggt ctccggaccg
      481 agagttttgc agcgctgtgt cggatggaaa tgggcatcgg ctcctgaagg aagtcactat
      541 cgggccggac ggcgggctta aagatgtggt ggtggtcctg gagggcgtcg agagaggcaa
      601 gccgttcacc tttaccgatg ccgaagtgga agcaagtctc tgccagttcc tgccgttcgt
      661 caccgtggtc agcgacaagc gtcgagtcac ggtgttcaat cgagatccgg ttttacacga
      721 tattcaaggg tatgcgtaca atgaagcagg cgtcgatatt gtcctccagc ggcctgcgct
      781 gcatgagagc gggaccaccg atatcgtgaa actggtcaaa gggcggaaag tgtttaccat
      841 gcaatgcggc atgcatccct acatgcagaa ttgggggtac gcgatcgaca atccctatta
      901 tgcgatcacg gacctcaatg gatcgtttgc catcggtgac atcccggcag gcacatacca
      961 cctcaaggcc tggcacccga ttttaggaac tcaagagcgg gatatcaccg tgaaaccgaa
     1021 cgagaccatc tcgcttgatt ggctgtttga cgcgaaacga gtcatcgaat gatcaaaggt
     1081 ccccacgctc tgtgtctcgc gatcgttctc ggcatgccga cggtcggcac acttgccgcg
     1141 cagcccgaca gtatcacgtc gtcgggacgg gttgaagtgg tcatggcgaa cgagcaccgc
     1201 aaggatctgg aggagatcag gaaggagttc gccgaggcgg gcctcacgaa tcttcatgtg
     1261 cgtcggtcct ggcagggccg ccgcaagtga tccaagagca cggcaaggcg atgcttcgcg
     1321 atgcggagga catggtcatg cacggaggca tgggcgacgg cggcgccatt aatccaagag
     1381 gccatcaccc actgtaagcg ggtggcggac atgggcgaca aagtcgatcc cggcgcgagc
     1441 ttgaatcccg ccacgaaagc tcgcgcggcg gcgaaagaag cgatgaagca tctcatggcc
     1501 atgagagaca atggggccta gggactggct ccggcacagc cgatgcctct cgcttttctc
     1561 aaacattctc ctgtcctggg ctgattttct ccgataaacg ggtaagataa gcgtgctatc
     1621 gtagtctgcc tatcgctctg gtacgcatga tcggatgacg tcggtacgtt gaaagacatc
     1681 gagaagctcg aatctgacct ctggaacgcc gccgatcatc tccgcgccaa ctccaagctg
     1741 acatccagcg attatttcat gccagctgca gaacctcgcg gccgcttctg aacacattcg
     1801 attcctcaaa cagcagaatg tcaaactccg cgtcgcccgc gacctgctcc tgcctcgtct
     1861 gatgagcgga gaaatcgccg tgtgatgtga cgcattctat ttcttaaaga tggtttctaa
     1921 agccatcgag aagaagtata atgcggtcat gaaaacaagg cttgggcggc aggaaaccca
     1981 actcctcgcc tatcttcagc tccgaaagcg gcggactgtc cggaccggtg agctgacggg
     2041 ccccctacgc ctgaaccgag accaagaacg ggagttgttc cgccggctgt cccgtggcgg
     2101 cctcattgca cgtgtccggc cgggcctgta cctcgtcccg gcacatctgc cgctcggtgg
     2161 agtatgggcg cctaatgaga ttctcgcctt aaacaccctg atggaagatc ggaacggccg
     2221 ctaccagatt tgtgggccga atacgtttaa tcgttatggg ttcgacgagc agataccggt
     2281 tcgcgtgtat gcctacaaca atcgtatttc cggtgaacgc acgattggat cgatcgtgtt
     2341 tcacctcata aaagtagccg atgcccggct cggcagcacg gaagagacca agaccgcgga
     2401 gggcgaaaca gcggtgtatt cctcacgagc gcggacattg gtggacgcgg tctacgattg
     2461 gtcgcgattc aatagcctgc cgagaggcta tcgatggatc gagcgtgaac tcgtggccaa
     2521 gcgagtgacg gctgcagacc tggtgacctt gactctccgt tatggagaca tcggcacaat
     2581 tagacgggtg ggggtgttgt tggaaagaga aggggtgcct cagccgttgc ttacgaaact
     2641 ggaagaggcg ttgtcctcct cgacaggaat gattccatgg attccgatcg ctcctaaacg
     2701 cgggacggtt gatcggcgct ggggcgtcgt catcaacgat ggagtatgat ccacgatgcg
     2761 cgatctgttt agggaagtcg aactctttcg agaagccctc atcatgacac agcaacaata
     2821 tgggttccct gagcggctga tcgaaaagga ctatttctgt tcgctcttgc ttgagttgct
     2881 gaacagcgcc catggcgacc tcgtgttcaa aggcgggacg tgtctggcca aggtttacgc
     2941 cgatttctac cgcttaagcg aggatttgga ctttgtaatc cccatgccga ccgatgcgcc
     3001 gcgatcagca cgaagcaggc aggcgacgaa gctcaaggac atcattgctt cgctgccgcg
     3061 cgagtgtccc tatgtgcgtg tgattgagcc gctgaccggc gcgaacaact ccacgcaata
     3121 caatgccgtg atgggctata cgtcttccat cgggcgagga gaagaaacga tcaagatcga
     3181 agtgggactc cacgagccgc tattgcttcc gcctcttaac ggcgaagcga agacgatact
     3241 gctcgaccca gtgtccggtc aggcgttggt tcccatgaag gtgagatgta tctcccagag
     3301 agaggccttc gccgagaaat ttcgcgcggc tttgagccgt cgagaggtgg cgatccggga
     3361 tttcttcgac atcgactatg ccgtccgtac actcggcttg cagccgaacg atgagactat
     3421 ggtcaagttg gtccaagcta aggtgggcgt atcccggaat gagccagtca atgtgagtgc
     3481 cgaacgtctt gccattcttc gacaacaagt tgacgctcag ttgagacctg tccttcgcga
     3541 gcgggacttt cgtgcgtttg atctggatcg agcattccga acggtggcaa acatggcgga
     3601 ccgcttgact gcgcgcttat gattaccgac atccacagcg aagatcggtt ggtccaacag
     3661 gccttcatca ggttcctgcg cgagcggctc ggatgggaaa acgtctcccg ggaagaaggc
     3721 aatcagtgaa gatgtcgtat attgggctca acctttccct ttcggcacca acgggctcac
     3781 ggcattctga tctctacccg cctcatagga tttcaacatc cgtgtgatcg tcggcatcag
     3841 ttggtctgtg cgatcggcgg gcattttaat ccgcacgccg cgcccttcgt tgcgccccaa
     3901 ggacaaaatg tggacttcca gcaccgcgtt gcgcttgtcg tcttgatagg tgaaggctag
     3961 gcgcttcgcg gggaatcccg ccagggtcgc ctcctgatcc ttctgccagg tgacctgaac
     4021 ctgcttcttg tcatacaggt ccgtgatgat gcgtcgatgg gcatcggcga attctttgag
     4081 tgtctgccgt ccgtcctggc tgcggccgct gaccacattc atgtggccgg atagtgcgac
     4141 caggctctgc tcgatcggag ggtacagtgt caccccgatt ccgtccggca gggggttgcc
     4201 aaggcgccag tcgtctggat agcggacgga aaaaccgaag cgatcgttcg tatagagctt
     4261 ccagccggtc ccctcgttcg tgtcggtcac ggcgcccaga gcaggcgtgg cgtgtgggac
     4321 gagactcatc gctaccaggg caaacataag atattcccga atcatcagca gcttcataag
     4381 gcagatgtat cctccattac cggatcgact tggcacagcg aaacccgacg gtcgcagctc
     4441 ggggacagcg ccgctccgcg tcgccgttcg ccccatggtt ttc
//
LOCUS       DATMTI010000853         1737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_329339, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000853 DATMTI010000000
VERSION     DATMTI010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329339"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /gene="flgM"
                     /locus_tag="VN039_13540"
     CDS             <1..294
                     /gene="flgM"
                     /locus_tag="VN039_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HWV47023.1"
                     /translation="GHGKVDHLAKLLLGVQETEGSGGRQAAARPQVGKDEVQISAQAK
                     ELQRIRELANQPDSERTEHVERIRRALDSGTYDVSGRAVGDALMKQVLTDAIL"
     gene            417..932
                     /locus_tag="VN039_13545"
     CDS             417..932
                     /locus_tag="VN039_13545"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HWV47024.1"
                     /translation="MPMSTFLPGIIQLCRLLHQEEATCHQLLETVHAERAAIRTLAIT
                     EFHPINCRRLSILESLQSLADERQQLVQELAVHYGLPPVTTSIQELIDNVQDSASEDL
                     RTTYRNYMTVAKTVRDEIKQNVVLIEGIRGVVDQALSTGAPAVPGGDLYTAAGQSFVA
                     GRVNMLIHQQG"
     gene            935..>1737
                     /gene="flgK"
                     /locus_tag="VN039_13550"
     CDS             935..>1737
                     /gene="flgK"
                     /locus_tag="VN039_13550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HWV47025.1"
                     /translation="MIGLTSLFDIARSALTTSQQALAVTGQNVANVNTPGYSRQEAVL
                     TERPPLNGQPGMVGTGVQVTSIRRYTDQFVNRQLTTVQQNLGRLSVSQEELSRLQNLF
                     GDSDDQGIAARMNDFFSGLQDVSTNPGEPAARSVLLANAGQLASTMNQASSDLTTERQ
                     SLNFQVQQTVTEINSLSKQIAELNSQIVSAEATGQNANDLRDQRELALNDLATRIDIT
                     TVESGTGALTVFAARGQVLVDGSTVRELSAIENPDNDGLFSIGYSTGGTR"
BASE COUNT          401 a          487 c          492 g          357 t
ORIGIN      
        1 ggtcatggga aagtagatca tctcgcaaag ctgttgcttg gggttcagga aaccgagggt
       61 tcaggggggc gacaagctgc tgcccgccct caggtcggta aggatgaggt ccagatttcc
      121 gcccaagcga aggagcttca acggattcgg gagctggcca atcagcctga ttccgagcgg
      181 actgaacatg tggagcgcat caggcgcgct cttgacagcg gtacatacga tgtcagtggt
      241 cgtgcggtcg gagatgccct catgaagcag gttctgaccg acgccatact gtagcgtccg
      301 cctttccagg ctcctgaaca ctcactcgcc atcgtccctt ccatcactcc gagggaaaga
      361 gggggaggca atggatggcg gaatgcagcg cacccccatg atcacaagga gtctgtatgc
      421 ccatgtccac gttcttgccg ggaatcatcc agctctgccg cttgttgcac caagaggagg
      481 caacctgcca tcagctgctt gagacggtcc acgcagaacg agccgccatc cggacactcg
      541 ctatcaccga gttccatccc atcaactgcc gtcggctatc tattctagaa tcgttgcagt
      601 cccttgccga tgaacgacag caacttgttc aggaattggc ggtgcactat ggcctgccac
      661 cggtaacgac atcgatccag gaacttattg acaatgtcca agactcggcg tcagaggact
      721 tacgaaccac ctaccgaaac tatatgacgg tggctaagac cgtccgcgac gagatcaagc
      781 agaatgtggt gctcatcgaa ggtattcgcg gcgtggtcga tcaagcgctg tcgaccggtg
      841 cacctgccgt tccaggcgga gatctttata cagcagctgg acaatcgttt gtcgcaggtc
      901 gtgtcaacat gcttattcat cagcagggat aattatgatc ggcctcacat ctctctttga
      961 cattgcgaga agcgcactta cgacctcgca acaagccctc gccgtcactg gacagaacgt
     1021 tgccaacgta aatactccgg gatattccag gcaggaagcc gtactcacag aacgtccgcc
     1081 gctgaacgga cagcccggca tggtagggac cggtgtccag gtcacgtcta tcaggcggta
     1141 tacggaccaa ttcgtcaacc ggcagcttac gaccgtgcag cagaatctcg gacggctgtc
     1201 ggtgtcgcaa gaagagttgt cccgtctgca aaatctattc ggagacagcg acgatcaagg
     1261 catcgccgca cgcatgaacg attttttcag tggtctacaa gatgtgtcca ccaatccggg
     1321 cgagccggcg gcccgttctg ttctgttggc aaacgccgga cagctggcgt cgactatgaa
     1381 tcaggcttcc tcggatctca cgacggagcg acaatcgctc aactttcagg tgcagcagac
     1441 agtgacggaa atcaacagcc tgtcgaaaca aatcgccgaa ctaaatagcc agattgtttc
     1501 ggccgaggcg acgggccaga acgccaatga tctgcgggat cagcgtgaac ttgccctgaa
     1561 tgacttggcg acgcgcatcg acatcacgac cgtggagtcc ggcaccggtg ctctgacggt
     1621 ctttgcggca cgtggacagg ttttagtgga cggatccact gtccgggagt tgagcgctat
     1681 tgagaatccg gacaatgacg ggcttttttc aatcggctat tctacggggg gtacgcg
//
LOCUS       DATMTI010000854         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_625867, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000854 DATMTI010000000
VERSION     DATMTI010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625867"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VN039_13555"
     CDS             <1..293
                     /locus_tag="VN039_13555"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HWV47026.1"
                     /translation="PDIISELREYGVTVLVHDPIAENEEAVAEYGIHLLEWEKMKDLD
                     GLIVAVAHKKFSDMSVADLLKPLRNKEQGVVIDVKSILDPDHIPASTRYWRL"
     gene            complement(520..783)
                     /locus_tag="VN039_13560"
     CDS             complement(520..783)
                     /locus_tag="VN039_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47027.1"
                     /translation="MVTTLPVQLLNLWRFYDDRAEAELLIKQLNEDYALSSIYLHPSL
                     HGQRDVLPSASVRLQLDDLDSSDSVCHQNFSMPRCSHFDTTFW"
     gene            complement(990..1313)
                     /locus_tag="VN039_13565"
     CDS             complement(990..1313)
                     /locus_tag="VN039_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47028.1"
                     /translation="MLLALLYPMILGPERIETIQLLRPNGVFQDLTWLHAYPNLSTVR
                     RFLLPVAPTALPKLRALHDRFLMHMTARPHRLTWLIFDIDSTILVVYGNQERARVDYN
                     HRLVL"
     gene            1627..1797
                     /locus_tag="VN039_13570"
     CDS             1627..1797
                     /locus_tag="VN039_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47029.1"
                     /translation="MEPFDEAVRRGFAWLDVAQLDASSVRPPGDQPISKQFGAIVEPN
                     CLGEPRHATTWT"
     gene            complement(1910..2194)
                     /locus_tag="VN039_13575"
     CDS             complement(1910..2194)
                     /locus_tag="VN039_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWV47030.1"
                     /translation="METVIRSTFWHNRHPSPEVPLRQSKFTDTPIVSILKEAEAGRPG
                     NEIWRQDGISSATDSKWQATDGGLEASDVKRLKELAYEHNRLKRLYAELS"
     gene            2210..2506
                     /locus_tag="VN039_13580"
                     /pseudo
     CDS             2210..2506
                     /locus_tag="VN039_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2535..3191
                     /locus_tag="VN039_13585"
                     /pseudo
     CDS             2535..3191
                     /locus_tag="VN039_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038789.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(3188..4057)
                     /locus_tag="VN039_13590"
     CDS             complement(3188..4057)
                     /locus_tag="VN039_13590"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HWV47031.1"
                     /translation="MTPAERRDLATHLVTVTGLPVQRACCAVRLGRATYYWPWVERAQ
                     RDAPVIAALTAVVAAHPHWGFWKCYRRLRLDGHPWNAKRVWRVYRNLRLNLPRRTKKR
                     LPLRPRQPLTVVPELNTVWAMDFMSDTLYSGRRFRTLNILDEGIREGLAIEIDTSLPA
                     ARVIRVLEQVVSWRGHPQALRLDNGPELVADQFMRWCVERGIELRYIQPGKPDQNAFI
                     ERFNRTYRTEVLNASVFESLEQVREISAEWLQSYNEERPHDALAGLPPAMYRAQFEAG
                     NSLLEVGAGIAKR"
BASE COUNT          920 a         1194 c         1230 g          842 t
ORIGIN      
        1 tgcctgatat catctccgag cttcgtgaat atggagtcac ggtactcgtg cacgacccta
       61 tcgcagaaaa tgaagaagcc gtggcagagt acggcattca ccttctcgaa tgggaaaaaa
      121 tgaaggatct tgatggatta attgttgccg ttgcacacaa aaagttttcc gatatgagtg
      181 tcgccgatct actaaagcct cttcgcaata aagagcaagg tgtcgtgatc gacgtcaaga
      241 gcatccttga tccagaccac attcctgcat cgacaagata ctggcgtcta tgaagacacc
      301 gggatattcg aggagcagga gaagcgccaa gcgagccatc aggatatgcg ctgtaatcca
      361 ttcaatacac ttcagtctga atcctgcatg caaagtgctg tcttgagagt ttgagtcact
      421 cgacatggtg gaaggcatat tgccaggtgg ttccccgagc gccgcttgcg gataaggcca
      481 agcagggtcg gttgtcggtt cgacgaagca taaccggcat caccagaatg tggtgtcgaa
      541 gtggctgcaa cgtggcatgc tgaaattctg atggcagaca gagtcgcttg aatccaggtc
      601 atcaagttgt aggcgaacag aagcagatgg aagtacgtct cgttggccat gaagtgacgg
      661 gtggagatag atgctgctca gggcgtaatc ctcgttgagc tgtttgatga gcaactccgc
      721 ctcggcacga tcgtcataga agcgccagag gttcagcaat tgcactggca gagtcgtgac
      781 caagacttgc tcgtgatatg agccaagctt gaaaagtgtc agttgctcgc tcggttcttc
      841 tgctggggcc ggcggacgac gacaaagcga tagagacgcg gccactgaat gagccgatcg
      901 cggaattcct ccacttcgat gcccacggct gaaactcaca gggccatcag acggacgaca
      961 atcacgaacc gagcccgctg cgcgtcgagt cataggacca gcctgtggtt gtagtccacc
     1021 cgtgcgcgct cctggttgcc atagacgacc aggatggtgg agtcgatatc gaagatcagc
     1081 catgttagcc ggtgtggtcg tgccgtcata tgcatcagga agcgatcgtg caaggcgcgc
     1141 agttttggca gtgccgtcgg ggccacaggc agcaagaatc gccgcactgt ggacaggttc
     1201 ggataggcgt gcagccacgt gaggtcctgg aaaacgccgt tcggccgcag caactggatc
     1261 gtctcgatgc gttccggccc aaggatcatc ggatagagca atgccagcag catttagccg
     1321 acgctgtaac gattgctccg ctgcgtcact cggaatttgg cgggcaatcc ccgtccttga
     1381 agccgaagcg cgacaagaac cgatgcagga gacatacgct gtaagctccc ctgtcaatga
     1441 gacacgggcc atggagaacg ttccgcgttc aggtgagccc gatacgtggc cggcggtatg
     1501 ccaggccagc tacggcatca tggggccgcc cttcgttaga ggtcaggaac cactcgccct
     1561 gctctcccgc acgtgatccc gggactccaa cacagaagca ctcagcactt cggtccgata
     1621 cgtccgatgg aaccgttcga tgaagccgtt cggcgcgggt ttgcctggct ggatgtcgcg
     1681 caactcgatg cctcctcggt ccgcccccca ggcgaccaac cgatcagcaa gcagttcgga
     1741 gccattgtcg agccgaattg cctgggggag ccccgccacg caaccacctg gacctgacag
     1801 gcccgctgga ccggaagacc gttcacccta accagatggg gcacgatctc ccgccgttca
     1861 gccggccgta ccgcttttgg gcgatgacat cctgcagtgc cgcgttcgct caggagagct
     1921 cggcatacag ccgcttcaac cgattgtgct cgtacgccaa ttccttgagc cgcttcacat
     1981 ccgaggcctc cagtccgccg tctgtcgcct gccacttgga gtcggtggcg gaactgatcc
     2041 cgtcctgccg ccagatctcg ttgcccgggc ggcccgcctc ggcttctttc aaaatggaga
     2101 caatcggtgt gtcggtgaac ttcgactggc gcaggggaac ctccgggctc gggtgacgat
     2161 tgtgccagaa cgtgctcctt atgactgtct cgatctaggg gggagcttac acttcggcat
     2221 gaaagcccat atcggagtgg ataaccagac caagctgatc cattcggtcg cggccatggc
     2281 ggcgaatgtg catgacagcc aagtgttgcc ccagctactg catggacagg agatgcgggt
     2341 gtggtggggg gatgccgcct ccagcaggca acacgccgtg atccgggaac atgcccccga
     2401 tgcccagagt ttcatccagg agaacgcccc tcgccatcgg cccttgagcg aggcggagtg
     2461 taagcttccc cgtcaaggag acacttcaag gggctggcat agctaaagcg acctgagagg
     2521 ggtagcttta gcgaatgatc tatcagcggc ggagccggtt gagtgggaaa cagcaagggc
     2581 ggctcatcga tctatttgtg gcaggcgcaa cagcgcgggc tgcggccgag attgtcggtg
     2641 tgaatcggaa taccgcgcga acgttttacc atcggctacg ccggttgatt gccagtaagc
     2701 tgcccagcta ggacttgtct ggcgaggtgg aggcggatga gagttacttc gggggtgttc
     2761 ggaaaggcaa acgtggccgg gcggcgggga aggtgccggt cttcggcttg ttaaaacggg
     2821 gcgggaaagt gtttgccgcg attattccga atgctcgatc gagaacgcgg ctgcccatta
     2881 ttcgtgagca ggtgccacca gacagcatcg tctatacgga tagcttcacc gcgtatgagg
     2941 tgttggatgt ctcagcgttc caccatcggc gcgtcaatca cagcaagatc ttcgtaacca
     3001 agggcgggca tcacatcaat ggaatggaga acttctggaa ccaggcaaag cgacagttgc
     3061 ggcggttcaa tggcatcaca ccggacaact tttactggtt cttgaaggag tgtgaatggc
     3121 gcttcaataa cggcagtcat caacagctgc tcacgcagct taaatactgg tacaaatcaa
     3181 ccaaacatta gcgcttagct atgccagccc ccacttctaa aagagaattt ccggcttcaa
     3241 actgagctcg atacatggct ggcggcagac ccgccagcgc gtcatgaggc cgctcctcgt
     3301 tgtaactctg taaccactcg gcactgattt cccgcacctg ctccagcgac tcaaacacag
     3361 aggcattgag cacttcggtg cgataggtac gattgaaccg ctcaatgaac gcattctgat
     3421 cgggtttgcc tggctggatg tagcgcaact caattccgcg ttcgacacac caacgcataa
     3481 actggtcggc caccagttct gggccgttat ccaaacgcag tgcctgggga tgacctcgcc
     3541 agctcacgac ttgttccaac acccgaatca cgcgtgctgc cggcagcgac gtatctattt
     3601 cgatggcgag cccttcacgg atgccctcat ccagaatatt gagggtccga aaccgtcgtc
     3661 cgctgtagag cgtatcactc ataaaatcca tggcccagac ggtattgagc tcgggcacca
     3721 cagttagggg ctgccgggga cgaagcggca gccgtttctt cgtgcggcgg ggcagattga
     3781 gccggaggtt gcgatagacg cgccagaccc gtttcgcgtt ccacggatgg ccatccagtc
     3841 ggagccgccg ataacacttc cagaatcccc agtgaggatg ggcagccact acggctgtca
     3901 acgccgcaat cacgggcgca tcgcgctgcg cccgctccac ccacggccag tagtaggtgg
     3961 cgcggcccaa ccttaccgcg caacaggccc gctgaactgg caacccggtc accgtcacca
     4021 agtgggtggc caggtcccga cgttcggcgg gcgtcagagc ttttttgcga tgacgtcctt
     4081 gagaccctga ttctctaagg acaactcggc gtacatccgc ttcaggcgac tattctcgtg
     4141 ttccagctcc ttcagccgtt tcacgtcgga ggcctccagc cccccg
//
LOCUS       DATMTI010000855         1704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_286149, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000855 DATMTI010000000
VERSION     DATMTI010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286149"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VN039_13595"
     CDS             <1..877
                     /locus_tag="VN039_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HWV47032.1"
                     /translation="YRLLKKHNLVGKEILPMPERKRFAYDQINELWQGDLSHGPTIRV
                     HGKAQKTFLIAYIDDCSRFVPYAQFFPSEKFDGLRIVTKEAVLRCGKPKRIYSDNGKI
                     YRSEVLQYACAEMGITLIHTQPYDPQSKGKIERFFRTVQTRFYPLLELNPPKSLDELN
                     ERFWKWLEEEYHRKPHASLDGKTPHEVFQSQVHLVSFIEDGDWLDAIFLKREHRKVKA
                     DGTITLNKQLYEVPPRFIGQSIELRYDERGVYVYEEGKRVAEAIRVRLEDNAYVKRHR
                     SPFAAIPAKEGEHSV"
     gene            870..1670
                     /locus_tag="VN039_13600"
     CDS             870..1670
                     /locus_tag="VN039_13600"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV47033.1"
                     /translation="MYKSFYSLSREPFAKETDPSEAYQGASFQEALRALEYVKRTRGI
                     GLLIGEPGAGKTFALRALKESLNPSLYHVVYFPLSTGGVMDFYRGLALGLGEEPKYRK
                     VDLFRQIQQAIERLYHERRITPVFILDEMHLAKDAFLQDIAILFNFEMDSTNPFVLIL
                     AGLPHLQGKLRLNQHRPLDQRIIMRYRMGPLEKEEVAGYIEHRMKQAGAKHPIFTPSA
                     LEAIALQSRGWPRVINTLATTCLLYGYQLKKDAIDEEVVRMAAEEMGY"
BASE COUNT          411 a          407 c          515 g          371 t
ORIGIN      
        1 ataccgattg ttgaaaaaac acaacctggt gggaaaagaa atcttgccga tgccggagcg
       61 aaagcgcttt gcgtatgacc agatcaatga gctttggcaa ggggacttat cccatggacc
      121 cacgattcgc gtccatggga aagcccagaa aacgtttttg atcgcgtata tcgatgactg
      181 ctcgcggttt gtgccgtacg cgcaattttt cccttcggag aagtttgacg ggctgcggat
      241 cgtcacgaag gaagcggtgc ttcgttgcgg gaagccgaaa cgcatttact cggacaacgg
      301 gaaaatttat cggtccgagg tgctgcagta tgcgtgcgcc gagatgggaa ttacgctcat
      361 ccacacccag ccgtatgacc cgcaaagcaa gggaaaaatc gaacggttct tccgcaccgt
      421 acagacacgg ttttatccac tgttggagct gaatccgccg aaatcgcttg acgagttgaa
      481 cgagcgtttt tggaagtggc ttgaagagga gtatcatcga aaaccgcacg cctcactgga
      541 cggaaaaacg ccgcatgagg tgtttcaatc gcaagtgcat ctcgtgtcgt tcatcgaaga
      601 tggcgattgg ctggatgcga tttttctgaa acgggagcac cgcaaggtga aagccgatgg
      661 cacaatcacc ctgaataaac agctatacga agtgccgcct cggttcattg ggcaatcgat
      721 tgagctccgc tatgacgaac gaggcgtcta tgtgtacgaa gagggcaaac gggtggcgga
      781 agccattcgg gtgcgtttgg aggacaatgc ctatgtaaaa cgccatcggt ccccgtttgc
      841 ggcgattccg gcgaaggagg gagaacacag tgtataaatc gttttactcc ctttcgcggg
      901 agccgtttgc gaaagaaaca gacccgtccg aggcgtatca aggggcgtca tttcaggaag
      961 cgttgcgggc gctggagtac gtgaaacgaa cccgggggat cgggctgttg atcggagagc
     1021 cgggggcggg caagacgttc gcccttcggg cgctgaaaga gtcattgaat ccatcattgt
     1081 atcatgtcgt ctactttccg ttatccaccg gaggcgtgat ggatttttac cgtggactgg
     1141 ccttgggatt aggagaggaa ccgaagtacc gcaaggtaga cctcttccgc caaatccagc
     1201 aggcgatcga gcgattgtat catgaacggc ggatcacgcc ggtgttcatt ttggatgaga
     1261 tgcatttagc gaaggatgca tttttacagg acatcgcgat cttgttcaac tttgagatgg
     1321 attcgaccaa cccatttgtc ttgattttgg ccgggctgcc ccatttacag gggaagctgc
     1381 ggctcaatca gcaccgccct ctcgaccagc ggatcatcat gcgataccgg atggggccgc
     1441 tggagaagga agaggtggcg ggctacatcg aacatcggat gaaacaggcc ggggcgaagc
     1501 atccgatctt caccccatcg gcgttagagg cgattgccct gcagtcgcgg ggatggcctc
     1561 gggtgatcaa cacgttggcc acgacgtgcc tgttatacgg gtatcagctg aaaaaggacg
     1621 ccattgacga agaagtggtg cgcatggccg cagaggaaat ggggtattag cacgaaaggg
     1681 gccgaatcgg cctcttttgt gctt
//
LOCUS       DATMTI010000856         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_116382, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000856 DATMTI010000000
VERSION     DATMTI010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116382"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1232
                     /locus_tag="VN039_13605"
     CDS             150..1232
                     /locus_tag="VN039_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWV47034.1"
                     /translation="MIEQSLPEIASSKSSPLFGFWYPAIPSHTLRSGTMKGLQMLGLP
                     IVLCRDSAGGLVAMRDICPHRGMPLSFGRFDGERVECSYHGWQFDLKGRCRCIPALPE
                     QGNLQTDKIGVMTYPAEETDGMIWLYLADERGNIDPLPPVPRMPLPSEPRQSFHISLT
                     YTCSADDGIIGLIDPVHGPYVHSWWRSDARMHEKTKIFEPISNGFRMIPHRPAKNSGP
                     FHWVERIYGGPLSTTIDFVLPNQRVELMQCGRAWVANRLMATPVSDMECRIDFSAYWV
                     GLHWLPFGNLIFRTLAKMFFGQDERAMKHLAVGLRHKPSAMFLGDADMPAKWYYKLKA
                     AHQASVQTGRPLDHPLKERVTLRWRS"
     gene            complement(1272..>2224)
                     /locus_tag="VN039_13610"
     CDS             complement(1272..>2224)
                     /locus_tag="VN039_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWV47035.1"
                     /translation="YQIFLAVSVCSMIITPFLMQLSPHLARRVEAVQRLHHWFPGQTT
                     ARVLETEGRHLRIKDHVIIVGYGLNGRNLARVLGETEVPYIALDLDGDTVRREAAHGL
                     PLYYGDATNPNVLRHVKIEDARVLVVAISDPFMARRTVQVARGLNPNIRIVVRTRYLR
                     ELEELHQLGVDDVVPEEFETSIEIFALVLRTYNMPQDLIVRKAEQVRREGYALLRRSE
                     LPELAHHLRGGTLADVEVETCRIEEDSPAAGKTIAQLALRPRIGTSIIALTRSGVTES
                     NPSEKTKLLAGDIVVLLGTRDQIRRAMGFILANEREDEQA"
BASE COUNT          460 a          630 c          621 g          513 t
ORIGIN      
        1 acagatcgtg tgctacatac accggacaac ttaatttgct atctacaaac tgtgttcgca
       61 tagcttgacc tatcgccatc ctctcgatat agtcggaatt cattgcggca tacccgtctc
      121 gcgagtttcc ttcctattga atggcccgga tgatcgaaca gtcattaccc gagatcgcat
      181 cttccaagag cagtccactc tttggattct ggtatccagc catacccagc cataccctgc
      241 gctcgggcac gatgaaaggg ctgcagatgt tgggtttgcc gatcgtcctc tgtcgcgata
      301 gcgccggtgg cctcgtggcg atgcgcgata tctgtccgca ccgtggtatg cctctgtcgt
      361 tcggccggtt cgacggtgaa cgagtggaat gttcgtatca tgggtggcag ttcgacttga
      421 aggggcgctg tcggtgcatt cctgccctgc ccgaacaagg gaatctacaa accgacaaga
      481 tcggtgtcat gacctatccg gctgaagaaa cggacggcat gatttggctg tatctggccg
      541 atgaacgagg gaacatcgat ccgttgccgc ccgttcctcg gatgccgctt ccgtcggaac
      601 ctcgacaatc gttccacatt tcgttgacct acacctgtag cgcagacgat ggcatcatcg
      661 gtctcatcga cccggttcat ggaccctatg ttcattcttg gtggcgtagt gatgcgcgta
      721 tgcatgagaa gacgaagatc tttgaaccga tctctaacgg ctttcggatg attccacacc
      781 gaccggcaaa gaacagtggc ccgtttcatt gggtcgagcg aatctacgga gggccgttgt
      841 cgacgacgat cgacttcgtg ctgccgaatc agcgtgtgga actcatgcaa tgtggaaggg
      901 cctgggtcgc taatcggttg atggccacac ccgtgtccga tatggaatgc cgcatcgatt
      961 tttctgcgta ttgggtcggg ctgcactggc tcccgttcgg caatttgatt tttcgtacgc
     1021 tggcgaaaat gtttttcggc caggatgagc gtgcgatgaa acacctagcg gtgggacttc
     1081 gccacaaacc ctcggcgatg tttctcggtg atgcagacat gccggcgaaa tggtactaca
     1141 aactgaaggc cgcccatcag gcatcagtcc aaacaggccg acctcttgat catcctctca
     1201 aagaacgagt gacgctccgg tggcgaagct gaccgcgcca tttcgggtgg cgctcatgct
     1261 gataccggtg gtcatgcctg ctcgtcttca cgctcattcg ccaaaataaa ccccatggct
     1321 cgcctgatct gatcgcgtgt tcccagcagc accacaatgt cgccggcaag aagtttggtt
     1381 ttctccgatg gattggattc cgtgacgccg ctccgggtca aggcgatgat ggacgttccg
     1441 attcgcggtc gtaatgccag ttgagcgatg gtctttccgg cagccggcga atcctcttcg
     1501 atccggcagg tctccacttc gacatcggcg agtgtgccgc cgcgaagatg gtgagccagc
     1561 tcggggagct cgctgcggcg taagagggcg tatccttcac ggcgaacctg ttcagccttt
     1621 cgcacgatga gatcttgcgg catgttgtag gtgcggagaa cgagcgcaaa gatttcaatc
     1681 gacgtttcaa attcttccgg cacgacgtcg tccacgccca gctggtgcaa ttcctccaat
     1741 tcgcgcaaat agcgtgtacg aactacgatg cgaatattcg ggttcaagcc tcgagccacc
     1801 tgtacggttc ggcgggccat gaacggatcg gagatcgcga cgaccaagac ccgtgcgtct
     1861 tcgatcttca cgtgcctcaa cacattcggg tttgtggcat cgccatagta gagcggcaag
     1921 ccgtgggccg cttctcggcg cactgtatcc ccgtccaaat ccaacgcgat gtagggcact
     1981 tccgtctcac ccaacacacg ggccagattg cgtccgttga gcccatatcc tacgataatc
     2041 acatggtcct tgatgcgtag atgccgcccc tctgtttcga gcacacgggc cgtcgtctgc
     2101 ccaggaaacc aatggtgcag ccgttgcacg gcctcgacgc gtcgcgcgag atggggggac
     2161 agctgcatca agaacggcgt gatgatcatg gaacagaccg agaccgccag aaagatttga
     2221 tatg
//
LOCUS       DATMTI010000857         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_203069, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000857 DATMTI010000000
VERSION     DATMTI010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203069"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VN039_13615"
     CDS             complement(<1..807)
                     /locus_tag="VN039_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47036.1"
                     /translation="MMKTRFISLPVVLSALIFLLDACATNPYLDNETRSTGRLTNGTV
                     LLHASAGPLSVGSGRVITGNDAAFHSKLELVQSATDSIEAMYYIYSDDLSSSVLTQAL
                     IDAAQRGVRVRLLVDYETNYNQLDLFSMMEKYGNIGKGSLEVRLFNRPTRAIVQDAVY
                     VTLGCGAALPNVDKTCGAMKNQEIDRMFQREQVEGRPAAELGISNLNIGNSGLFLSGL
                     YSKKSDLMALAVLAGQSVDVSSLKQPGEAPSVQQRAQLKRVAELYLHTRVG"
     gene            complement(909..>2342)
                     /locus_tag="VN039_13620"
     CDS             complement(909..>2342)
                     /locus_tag="VN039_13620"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV47037.1"
                     /translation="HKNEAAMRWTGSVLFGYGVAAILATGCATPFERQLGTTAEYHAI
                     LKTPVIAPSSEKLLQDGPDYLIGIEKGDTDVVQKLKPCAPDSPQGSLGYCLNLDAYPQ
                     DSPWRTRIARDNDHSKTLFVSHIARLDNIDHQKIGSKRVCFLYNIYPHSDFPEATDCG
                     RPFAAQTSRLPLGVMSGLTALEELEQELDERLAKEPPATRPTHVLVMSTGWNTLQAES
                     LDNYQHWTSHLKRAARDEARPFRPLVIGISWPSEWPLISSWLGPVISVFNKEHDADEV
                     GYITVNVLVHKVLNNLKRKHNVQIVVIGHSFGGRVMATALNSRALLFREGASHPTATL
                     MLGLQPAFSIQRFGDGDQGDPYRRFFQASRKYLLTSSTRDWILTFVGITNPVGSSRGL
                     AKAKERPERFEVVSLDKEGQWQREPSSNPCQLVVLDGSEAISGHHDVYGAAVGRLMWE
                     AIRSFAPASSWSAAPQSVQESPCTVGP"
BASE COUNT          550 a          628 c          635 g          529 t
ORIGIN      
        1 ccctacccga gtatggagat aaagctcagc aacccttttg agttgtgcgc gttgctgcac
       61 ggagggggct tcgccgggtt gtttcaagct gctgacatcg acggactgtc ctgctaagac
      121 ggccaaggcc atcaggtcgg attttttgga atataatccc gacaaaaata atccggagtt
      181 gccgatgttg agattggaaa tgccaagctc cgctgctggt cgtccctcga cttgctcacg
      241 ttggaacata cggtcaatct cctgattctt catcgctcca catgttttgt cgacattagg
      301 gagcgccgct ccgcaaccta atgtgacata gaccgcatct tggacaatcg cacgagtcgg
      361 ccgattaaac aatcgaacct caagtgaccc tttccctata ttcccgtact tttccatcat
      421 tgaaaacaga tccagctggt tgtagttagt ctcatagtcg acaagcaggc ggacacgtac
      481 tccgcgctgg gcggcatcaa taagagcctg tgtcaggacc gaggaggaca agtcgtcaga
      541 gtaaatgtag tacatggcct caatggaatc cgttgctgat tggacgagct cgagtttgga
      601 atgaaatgcc gcatcgtttc ctgtgatgac acggccgctt ccgacgctca gtggtcccgc
      661 tgacgcgtgc agcaagacgg tgccatttgt cagacgcccc gtcgaccgtg tttcgttgtc
      721 gagatagggg ttggtggcac aggcatcaag caagaaaatc agcgccgaca gcaccacggg
      781 taaggaaatg aagcgagttt tcatcatatc ctccaataca cggctagatg agaagacaca
      841 tcgggtttgg tacgacacag gaacagatgc gcgtgaaaac gtccgacggc gagcgcacgc
      901 acggacattc atggtccgac agtacagggt gactcttgaa cgctctgggg agcagcagac
      961 cacgaagagg ctggggcaaa ggagcgaatc gcttcccaca tgagtcgccc cacggctgca
     1021 ccatacacat cgtggtgccc actaattgcc tctgatccat ccaagaccac aagttgacaa
     1081 gggtttgatg aaggttccct ttgccactgt ccctctttat cgagggaaac gacctcaaac
     1141 cgttctgggc gttctttggc cttagccagg ccgcgtgaac ttccaactgg gttggtaatg
     1201 ccgacgaatg tgagaatcca gtcacgggtt gaggaagtaa ggagatactt ccgagaggct
     1261 tgaaaaaatc gccgataagg atcgccttgg tcaccgtctc cgaagcgttg aatgctaaac
     1321 gcaggctgaa gccccaacat caaggtagct gtcggatggc ttgccccctc tcgaaacaac
     1381 agcgcacgac tgttgagagc cgtggccata actcgtccgc caaaactgtg accgatgacc
     1441 acaatttgaa cgttgtgctt acgtttcaaa ttattgagaa ccttatgcac aagcacgttg
     1501 acagtgatat atccgacttc atccgcatca tgttctttgt taaacacgct gatgaccggc
     1561 cccagccagc ttgaaatgag cggccactcg gatggccaac ttattccgat gaccaatggg
     1621 cgaaatgggc gcgcctcatc tcgggcggct cgtttgagat gggacgtcca atgttggtag
     1681 ttgtcgagtg attccgcttg gagcgtattc catccggtcg acatgaccag cacatgcgtc
     1741 ggcctcgtcg ctggcggctc cttcgcaagg cgctcgtcga gctcttgctc caattcctca
     1801 agggccgtca accctgacat tacgcccaag ggaaggcgtg aggtttgcgc cgcaaagggg
     1861 cgaccacagt ctgtggcctc gggaaagtcg ctgtgcggat agatgttata gagaaagcag
     1921 actcgcttcg agccgatctt ctgatggtcg atattatcga gtctcgcgat atgcgaaaca
     1981 aacaatgtct tgctgtggtc gttgtcgcgt gcaatccgcg tccgccatgg gctgtcctgc
     2041 gggtaggcat ccagattcag gcaatagcct agactcccct gcggagaatc cggagcgcat
     2101 ggtttcagct tctggacgac gtcggtatcg cctttctcga tgccaatcag atagtccggt
     2161 ccatcctgaa ggagcttctc cgaagaaggg gcgatcacgg gagtcttcaa gatggcatga
     2221 tactcagccg tggttcctaa ctgacgctca aacggcgtgg cgcatccagt ggcgaggata
     2281 gcagcgacac cgtacccgaa tagaactgat cctgtccatc tcatcgcagc ctcattttta
     2341 tg
//
LOCUS       DATMTI010000858         1716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_541848, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000858 DATMTI010000000
VERSION     DATMTI010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541848"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..>1716)
                     /gene="rpoB"
                     /locus_tag="VN039_13625"
     CDS             complement(10..>1716)
                     /gene="rpoB"
                     /locus_tag="VN039_13625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWV47038.1"
                     /translation="GKDSGLDVYDLIKFQRSNQNTCITQTPVVGIGQPVKKGQVLGDG
                     PAIDHGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTK
                     LGKEDVTRDIPNVGEEALRNLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLL
                     RAIFGEKAGDVKDTSLTVPPGVEGIVVDVKIFSRKGLDKDERSKSIETDDQMKLQRDH
                     HEELRIIDEEKTKKIRKLLLGKVVGRDLMDPESGDVILKKKGKLTAEVLRRLPDDTVR
                     HIILSDPDEQKELEDVERRAKEQIEILQTLYDEKVGRLKRGDELPPGVIKLVKVYIAM
                     KRKIQVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILE
                     THLGWAAKALGIQVASPVFDGAAEKEIKDLLKKAKLPVSGQSQLIDGKTGEPFGSPVT
                     VGYMYVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAA
                     STLQEFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQSLGLDVELVKT
                     QD"
BASE COUNT          343 a          549 c          394 g          430 t
ORIGIN      
        1 cacaagcggt tagtcttgcg tcttgaccaa ctctacgtcg agccccaagc tctgtagttc
       61 tttgaccaat acattgaacg actccggcaa tccgggctcg aggaacggct cacctttgac
      121 aatcgcttca tacatgcgcg atcgacccgg cacatcgtcc gatttgacgg tcaggaactc
      181 ttgcagagtc gaggctgccc cataagcttg taacgcccag acttccattt ctcccaaccg
      241 ctggcctccg aattgagcct ttccgcccag aggctgctgg gtcacgagag aatagggacc
      301 gatagaccgc gcgtgaatct tgtcgtccac cagatggtgg agcttcagca catacatata
      361 cccgacagtg accgggctgc cgaacggctc accggtcttg ccgtcgatga gctgcgactg
      421 gccgctcacc ggtagtttgg cttttttcag cagatcctta atctccttct ccgctgcacc
      481 atcgaatacg gggctggcca cttgaatgcc caatgcctta gccgcccatc caaggtgggt
      541 ctctagaatt tggccgacgt tcatacgaga cggcacaccc agaggattca gtacgatttc
      601 aaccggggtc ccatccggca gatagggcat atcttcttcg ggtaggaccc gcgacacgac
      661 gcccttattg ccgtgtcgac cagccatttt gtccccgacc tgaattttgc gcttcatcgc
      721 gatgtagacc ttcacgagct tgatgacacc gggaggcaac tcatcccctc gctttaagcg
      781 ccctaccttt tcgtcgtaca gcgtctggag gatttcaatc tgttccttcg cccgtcgctc
      841 gacgtcttcc agttcttttt gctcatcggg atcactcagg atgatgtgcc gcaccgtgtc
      901 gtccggcaat cgcctgagaa cctccgccgt cagtttaccc ttcttcttca aaatgacatc
      961 gccgctctca gggtccatca gatcgcgacc gaccaccttt ccgagcaaca gcttccgaat
     1021 cttcttcgtc ttttcttcat cgatgatccg tagctcttca tggtgatcac gctgcaactt
     1081 catctgatcg tcggtctcga tgctcttcga ccgctcgtct ttgtcgagtc ctttgcgaga
     1141 gaaaattttg acatcgacca cgataccttc cactcccgga ggcacagtta acgaagtatc
     1201 cttcacatcg ccggccttct caccgaagat tgctcggagc aacttctctt cgggagtcaa
     1261 ctgagtctcg cccttcggcg tgactttccc caccagaatg tcaccgggct ttacctccgc
     1321 accgatgcgg atgatcccgc tctcatccaa atttctcagc gcctcttctc cgacattggg
     1381 aatatctcgc gtcacgtctt ccttccccag cttggtatcc cgggcctcta cctcaaactc
     1441 ttcgatgtgg atcgaagtga aagcgtcttc gcgaaccaat ttttcactga gcaatatggc
     1501 atcctcgaaa ttgtatccgc cccacggcat gaacgccacg aggacgttct tgcccaacgc
     1561 gagctctcca tgatcaatcg ccggtccatc tccaagaacc tgccccttct tgaccggctg
     1621 gccgataccg acgacggggg tctgagtaat gcaggtattt tgattcgacc gctggaactt
     1681 gatcaggtca tagacgtcca gcccggaatc ctttcc
//
LOCUS       DATMTI010000859         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_710210, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000859 DATMTI010000000
VERSION     DATMTI010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710210"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VN039_13630"
     CDS             complement(<1..247)
                     /locus_tag="VN039_13630"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HWV47039.1"
                     /translation="MQATTPTIDVESLLNAIPGGSSAGISLRYEAVYDALKEARRSDE
                     ELPQGEWVGKTKNADWELVVKLASEALSAKSKDLQVGV"
     gene            complement(289..1101)
                     /locus_tag="VN039_13635"
     CDS             complement(289..1101)
                     /locus_tag="VN039_13635"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HWV47040.1"
                     /translation="MTAVEQAKELVQGGLLHAAIESVTQAVKGDPVNGMLRTVLFELL
                     CFAGDWDRAEKQLDVIGHQDAKTEIGVLTYRQLIAGEKARRRVFEQGVQPHFFQEPPS
                     YIDRQLRALMERQQGLSEAAAKTLDEAMVQWPRLGGRINGRRFDEFRDADDTLGPVLE
                     VLARDQYAWLPFEQISRVEFQAPKRLRDALWVEARIEMKGHKLAQVFVPALYVQSSEQ
                     PDERLKLGRMTNWLALGNDRFQGIGQRMFWVDSSEESILGLRNIHIESNTRR"
     gene            complement(1126..2922)
                     /locus_tag="VN039_13640"
     CDS             complement(1126..2922)
                     /locus_tag="VN039_13640"
                     /inference="COORDINATES: protein motif:HMM:NF018470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HWV47041.1"
                     /translation="MLKLYLMLSEPSRLDRDYVAGKLRTLWDDLLTSHYGTDVPKGLP
                     EAIHGQIDLYARILDAGHLPFSTIDHNLVSRVRLALHAIPLPHRYFSRMQREAPSITL
                     SAFTPEPYRIDKAVAGQHQVVLTNTLAVPALFTPDGWRILFPFVRDKVLKEAGQEAWV
                     LNVEEVPPKEMEAAIERIYFDEYVRQWRRFVQGTRMLSAERLPDIANRLEVLSRKNSQ
                     LLALLKDVDRHTRLVSLTEQATGKFFDLLPGAKSSETPVQKNPVQLTFESFHEFVAPS
                     GEAKEAAVDKYMAELGKAASSLAVLAQAGPGDQDLKDPRDLRQARLATEVLLRQLEGE
                     VREDFRPLLVQPFLLAQATATKGELNDLNRAMKQDLAGLCSQTVASRYPFRKSDQPGG
                     DLTFTDLAAFFHPQSGAFWRFYQSKLKSSVQEENGRWVVKVSDMPVGPGFLETLRQAD
                     LISQGFFGKGTPEPRIAFELRPNGVPGVDEVRLHIDGRELRYRNEVEEWFQFVWPGTP
                     ETAGATLQVVSSGFTQPNTLHFDGRWGLFRLLDEAHVTQVRPTEYAVEWTVPGADGAP
                     VKARFDLKADTPKNLFVPGLFSRFRCPAQVGT"
BASE COUNT          619 a          911 c          795 g          618 t
ORIGIN      
        1 agacaccgac ttgcaaatcc ttggatttag ccgagagggc ctcactcgcg agcttgacca
       61 cgagttccca gtcggcattc ttcgtcttac cgacccattc tccctgcggt aattcctcgt
      121 ccgagcggcg cgcctcctta agtgcgtcat agacggcttc gtaccgcaac gagatgcctg
      181 cagaactgcc gccagggatg gcattcagca aagactccac atcaattgtc ggcgtcgttg
      241 cctgcacggt ggcttccata tcttgcggcg ccctttcttt ccagcgctct accgccgggt
      301 gttcgattca atatgaatgt ttcggagccc caagattgat tcttcggagc tgtctaccca
      361 gaacatccgt tgaccgatcc cctggaaccg gtcatttcca agcgccagcc aattcgtcat
      421 acgaccaagc ttcaaccgct catcgggttg ttcgctggac tgaacataga gagccggaac
      481 gaaaacctgg gccagcttat gccccttcat ttcgatcctc gcctcgaccc acagcgcgtc
      541 gcgtaatcgc ttgggagctt ggaattcgac gcgactgatc tgttcaaacg gcaaccatgc
      601 gtactgatcg cgagccagca cctccagcac gggtcccaag gtatcgtcgg catcgcggaa
      661 ttcgtcgaac cgacgaccgt tgatgcggcc tccgagccga ggccattgca ccatcgcttc
      721 atcgagcgtc tttgcggccg cctcggacaa accctgttgc cgctccatca gcgcccgtag
      781 ctgacgatcg atatacgatg gcggttcctg aaagaaatgc ggttgcacgc cttgctcaaa
      841 aacccgccga cgcgcctttt cgccggcaat gagctggcgg tacgtcagga ctccgatctc
      901 cgtcttcgca tcctgatgcc cgatgacatc gagctgtttc tccgcccgat cccagtcacc
      961 ggcgaagcag agcagttcga agaggacggt gcgcaacatg ccgtttacgg gatccccttt
     1021 gacggcttgt gtcacgcttt cgatcgcggc gtgcagtaat ccgccctgca ccaattcttt
     1081 cgcttgctcg acagccgtca tgagagtttc tcctccacaa aaacattagg tgccgacttg
     1141 agcaggacag cggaaccgtg agaacagccc ggggacgaac agattcttcg gagtatcggc
     1201 tttcagatcg aaccgggcct tgaccggagc gccgtcagcg ccgggcactg tccattcaac
     1261 ggcatactcg gtcggtcgca cctgcgtcac atgggcctca tccagcaaac ggaagagtcc
     1321 ccaccgaccg tcgaaatgca gcgtgttggg ttgtgtgaac ccgctcgaaa cgacttgcaa
     1381 cgtcgcgccg gccgtctcag gggtgccggg ccagacgaac tggaaccatt cctctacctc
     1441 attgcggtaa cgcagctcgc gcccatcgat atgcagccgc acttcatcca caccgggcac
     1501 gccgttggga cgcaactcga atgcgatgcg cggctccggc gtccccttgc cgaaaaagcc
     1561 ctgggagatc agatccgcct gacgcagtgt ttccaaaaac cccgggccga cgggcatatc
     1621 cgagactttc accacccacc gcccgttttc ctcctggacg cttgacttca gcttcgactg
     1681 gtaaaaacgc cagaacgccc ccgattgtgg atggaaaaag gccgcgagat cagtgaacgt
     1741 gagatcgccg cccggctgat cgcttttcct gaacggatag cgagacgcca ccgtttgcga
     1801 gcacaacccg gcgagatcct gcttcatcgc ccgattcaga tcattgagtt ctcctttggt
     1861 cgccgtggcc tgagcgagca gaaacggctg aaccaggaga ggacgaaaat cttcccggac
     1921 ctcgccctcc aactgccgca acagcacttc cgtcgcgagc ctggcctgcc tcagatcgcg
     1981 tggatctttc agatcttgat caccgggacc tgcctgcgcg agaacggcga gtgatgacgc
     2041 ggctttgccg agttccgcca tatacttgtc gacagcggct tctttcgctt cgccggacgg
     2101 ggccacaaac tcgtggaagg attcaaacgt cagttgaacg ggattttttt gaaccggcgt
     2161 ctctgatgat ttcgctccgg gaagcagatc gaaaaactta cctgttgcct gttcagtcag
     2221 gctgacaaga cgcgtatgcc gatcgacatc tttcaacaac gccaacaact gtgagttctt
     2281 gcgcgacagg acttccagcc gatttgcgat gtcgggcagc cgttccgcgg aaagcatcct
     2341 ggttccctgg acgaaccgcc gccattgcct cacgtactca tcgaaataga tccgctcgat
     2401 ggcggcctcc atctccttgg gaggcacctc ctcaacgttc aatacccagg cctcttgtcc
     2461 ggcctctttc aggaccttgt cgcggacgaa cggaaacaga attctccagc cgtcgggtgt
     2521 aaacagcgcc ggcaccgcga gcgtattggt caacacgacc tggtgctgcc cggcaacggc
     2581 cttgtcgatg cggtagggtt ccggcgtgaa tgcggaaagc gtgatcgacg gagcttcccg
     2641 ttgcatgcgc gaaaagtaac gatgcggcag aggaatggca tggagcgcca gcctgacgcg
     2701 gctgacaaga ttgtgatcga tggtggagaa aggaagatga ccggcatcca aaattctggc
     2761 atacagatcg atttggccgt gaatcgcttc gggcaatcct ttcggcacat cggtgccgta
     2821 gtgcgaggtc aacagatcgt cccataacgt tcggagctta cccgcgacat aatcgcgatc
     2881 caggcgactc ggttcagaga gcatgagata gagtttgagc aacgtttaat agttatcgct
     2941 ttc
//
LOCUS       DATMTI010000860         1605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_839795, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000860 DATMTI010000000
VERSION     DATMTI010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839795"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /gene="lpxC"
                     /locus_tag="VN039_13645"
     CDS             <1..976
                     /gene="lpxC"
                     /locus_tag="VN039_13645"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HWV47042.1"
                     /translation="SGWLCLSICLYEGGLVRNQQTLASAVTCSGVGLHSGQSASITLR
                     PAPPNTGVVFVSRRTDVDTYLSASIEHRVPTELCTAISGNGFQVQTIEHLLSALSGLH
                     VDNVFIDVSASEVPVMDGSAAPFVRLIQSVGIVSQERKQPFLKIMAPIEVAEGSKRVR
                     IEPSSTPRITYSIHYEHPLIRTQTYAYDCSVNAFENEIAEARTFGFLYEVQALWARGL
                     GKGGTLDNTIVLSDDGVVNESGLRFDDEFVRHKILDLIGDFSLLGAPFIGHIIADRSG
                     HALHTRLVQQILTQTDKWVLLNIESSGAEKRSAAHALHLQPAVALQAS"
     gene            complement(1192..>1605)
                     /locus_tag="VN039_13650"
     CDS             complement(1192..>1605)
                     /locus_tag="VN039_13650"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWV47043.1"
                     /translation="EIATIKKYLELAETLYRVEADKAKLASLSNQIITTDDSKGIRPV
                     PVMDVTDQSREILLGRSKYVGKSVPQAAYEILRESNRAMHAKELVQRLIEGGLQIKGK
                     TPLTSIATSLKRDKRFRKVGPNTFEALDDMLIQAV"
BASE COUNT          332 a          429 c          410 g          434 t
ORIGIN      
        1 atccggatgg ctgtgtttga gcatttgtct gtatgaggga ggacttgtgc gcaatcaaca
       61 aactttagca tcggcagtaa cttgttctgg tgtgggactc cattctggcc agtcggcgtc
      121 gattacgctg cggcccgccc cccctaacac cggtgtggtt tttgtcagtc gtagaactga
      181 cgttgatacg tatctttccg cctccattga gcaccgggtc cccaccgaat tgtgcactgc
      241 catcagcggt aatgggttcc aagttcagac catagaacat ctgctatcgg cgctgtccgg
      301 ccttcatgtc gacaatgttt tcatcgatgt atcggcaagc gaagttccgg tcatggatgg
      361 aagcgccgcg ccgtttgtcc ggctgattca gtccgtcggc atcgtctccc aggagcgaaa
      421 gcagccgttt cttaaaatta tggccccaat tgaggtggct gagggatcga agcgtgttcg
      481 gatcgaaccg tcctccaccc cgagaatcac ctactccatt cattacgagc atcctctgat
      541 cagaacgcag acctatgcgt atgattgctc cgtcaacgca tttgagaatg agatcgccga
      601 ggccaggacc tttggatttc tatacgaagt ccaagcattg tgggctcgtg ggctcggcaa
      661 aggaggaacc ctggacaaca ccattgtgtt gtcggatgac ggcgtcgtca atgaatctgg
      721 ccttcgattc gacgatgagt ttgttcgcca caagattctc gatctgatcg gtgacttttc
      781 tcttttgggg gcacccttca tcggtcacat tatcgcagac cgatcaggac atgcactcca
      841 cactcggttg gttcagcaga tcctgactca gacggataag tgggtgcttc tgaacataga
      901 atcctcagga gcagagaaac gatcggctgc tcacgcgctt caccttcagc cggccgttgc
      961 acttcaggct tcttagccta tagggatttt cggtatccgg ctgattgccg ttctttccgg
     1021 taggacgctc tcatccagaa catcacagca tgatacgccg ggggtaagtg gcctcacccc
     1081 cgacgtatca atcatggcgg gcattacttc ttcttcttct tcgctgcctt ctttgttgcc
     1141 aagagtctca cctcccttcg cattgtcaag ttaggtccat tgcctgattg gctatacggc
     1201 ctgaatcagc atgtcgtcca aggcttcgaa cgtattggga ccgacttttc tgaagcgttt
     1261 atcgcgcttg agcgacgtcg caatcgacgt gagcggcgtt tttcctttga tctgcaaccc
     1321 gccttcgatt aaacgctgca ccaattcctt cgcgtgcatc gcccgattcg attcacgaag
     1381 gatttcatag gctgcttgag ggacgctttt ccccacgtat ttacttcgac cgagaagaat
     1441 ttcccgtgat tgatcggtga cgtccataac gggtacaggt cgaatccctt tggaatcatc
     1501 cgttgtaatg atctggttgg agagactggc aagcttggcc ttatcggcct cgacacgata
     1561 gagggtttca gcaagctcaa ggtatttttt aatagtggcg atttc
//
LOCUS       DATMTI010000861         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_583559, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000861 DATMTI010000000
VERSION     DATMTI010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583559"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..2454
                     /locus_tag="VN039_13655"
     CDS             187..2454
                     /locus_tag="VN039_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47044.1"
                     /translation="MRCTMKVLRTSKLLLCGTVLGVGTALCVALPLDLTAEADDTTLE
                     RPGFSLVTRDGSGDAGLYTSIAIGADGLGLVSYYDTTNGDLKVAHCSNQMCTSATISS
                     LDRAGDVGRYTSIALGSDDLGLMSYYDATNGDLKVAHCVDIACTSATLKTLDHAGDVG
                     QYGSLTIGIDKLPLITYYDGTNGDLKIAHCTDVACEAATITVLTFLQAEENVGQYTSV
                     TIAGGQGLISYYDATDRYLKSALCPDLSCTEPTLTTTPDKTGDVGRFSSVAIGTDSRP
                     LISYYDATNGDLKVIHCEEVVCVSPTRTVLESEGNVGQYTSIVIGADGLALISYYDVT
                     NGDLKVAHCGDVACTSATLATPDRTGNVGSSTSVAIGTDGLPLISYYDVTNGDLKVAH
                     CGDVSCTDATVTPLDGAAINVGASSAMVLGADGKAVISYYDATNGDLRVVHCADLACA
                     SATLTTVQNQDNVGQDSSVAVGADELPLISYYDATNGDLRVAHCTDVACTMATTFTLD
                     QAGQVGQFTSMTIGMDGRGLISYYDATNGDLKVAHCDDVACSSAKITTLVSKGDVGQF
                     TSTTVGADGLALISYYDITNEDLKIAHCDDVACGSATESTLDSRGNVGRYSSVTIGSD
                     GLALISYYDGTNDDLKVAHCIDLTCSSAKVNTLHSKGSIGQFTSVTIGQDGRGLISYY
                     DAMNGDLRVAHCANVDCETATHNAVQSLDDVGRFTTIITGKDGRALISYYDVTNGDLK
                     VAHCSNSFCVPYTRH"
     gene            complement(2498..3817)
                     /locus_tag="VN039_13660"
     CDS             complement(2498..3817)
                     /locus_tag="VN039_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWV47045.1"
                     /translation="MNDIGRRGFLVRTGLALGAAVLASAYDRASADQQPPQSGFKSWD
                     DLRAQFPLSPQLIHLAAFFLASHPTPVRQAIERHRAGLDSDPIGYWYEHEEKEEAKVL
                     QAAADYLGAQPTDIALTDSTTMGLGLLYGGLRLQAGQEILTTIHDHYSTETSLRLRAE
                     RTGAKVRQIPLYRALKTVSRDEIIDSLRKGITPATRIVAVTWVHSSTGLKLPIHEMAQ
                     AIQAINRSRAEQDRVIFCVDGVHALGVEDFRLSELGCDFLIAGTHKWMFGPRGTGLVW
                     GHPHAWPMAQAIIPTFNSQAFEIWLKNGPPHGLPQSIYMTPGGFHSFEHRWALDEAFT
                     LHQTVGKSKVTQRIYELNQQLKQGLAAMPHVTLHTPISQDLSAGIVCFEVAGMAPRTV
                     VEKLRQRGIVGSVTPYATQYARLAPSLLNSPQEIEKTLVEIRNLYSS"
     gene            complement(3937..>6644)
                     /gene="gcvP"
                     /locus_tag="VN039_13665"
     CDS             complement(3937..>6644)
                     /gene="gcvP"
                     /locus_tag="VN039_13665"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HWV47046.1"
                     /translation="GEQAVLARLKGIASQNKVYRSLIGMGYYDCVTPGVIQRNILENP
                     AWYTQYTPYQAEISQGRLEALVNFQTMVADLTGLPLANASLLDEATAAAEAMAMCYTI
                     ARNAGEERKEFFVSRDCHPQTLAVLQTRAEPLGIVIKTGVASSVDCSSPQLCGILLQY
                     PATDGYVGDFSTLVAQAHEADVRVAVATDLLALTLLRSPGEFGADIAVGSTQRFGVPI
                     GFGGPHAAFLATKEEYKRQVPGRLVGISKDVTGKPAIRLSLQTREQHIRREKATSNIC
                     TAQVLLAVMAAMYAVYHGPDGLRRIAERVHGLTLLLGEGLRRLGFDVLPKVVFDTIRV
                     PVPKAQADQIATRANEHRINFRYHEDGSIGVSLDEVTSEEEVQRLLRIFVGHDHLPFR
                     LSDLAADIDFSYSSPLARTSKYLTHEVFHRYHSEHEMLRYLHRLQTKDMSLVHSMIPL
                     GSCTMKLNATAEMLPVTWPEFARLHPFAPGEQTLGYRTLFGQLESWLAEIAGFAAFSL
                     QPNAGSQGEYSGLMVIRAYHRSKGETHRDVCLIPVSAHGTNPASAAMVGMEVVVVACD
                     RNGNVDIDDLEAKAAQYRDRLSALMLTYPSTHGVFEAGVRRICQIVHTHGGQVYIDGA
                     NMNAMVGLCRLGDIGADVCHLNLHKTFCIPHGGGGPGVGPIGVARHLVPFLPGHPVVK
                     LGAPQAIGPVSAAPFGSPSILPISWMYIALMGRNGLTKATQVAILNANYMAKRLEKHY
                     SILYKGDSGLVAHEFILDLREFKDSAGIEAMDVAKRLMDYGFHAPTVSFPVAGTLMIE
                     PTESEAKSELDRFSEALILIRAEIQEIIDGRQPRTNNVLKNAPHTAAMVTATEWNRPY
                     SRELAAFPAPWLRSNKFWPSVSRIDEAYGDRHLVCSCPPMESYQS"
BASE COUNT         1451 a         1882 c         1908 g         1403 t
ORIGIN      
        1 cttgaagcta tcgggaaaat ctcacaagct tgtcatttca gcactgcgtg aggtgaacgg
       61 agcaagccgt ggagctccac cagtagactc tcatatttgt cgagatataa actgtacgag
      121 aaaagggtaa tacgacgagg gattcttcta gaactctcgc tatttctttg gcgaagaatc
      181 attggtatgc ggtgcacgat gaaggtgctg aggactagta agctcctgtt gtgcggaacc
      241 gttctcggtg taggaacggc actttgtgtc gccctgccac tggatcttac tgccgaagca
      301 gacgatacga cgctggagcg tccaggtttc tcgcttgtga ctcgagacgg ctccggcgat
      361 gccggtctgt atacttcgat cgccatcggg gccgacggtc taggcctcgt cagttactat
      421 gatacgacca acggggatct gaaagtggcg cactgttcaa accagatgtg cacctccgcg
      481 acgatcagct cgctggatcg cgccggggat gtcggccggt acacctctat cgcgcttggg
      541 agcgacgacc tcggcctcat gagttactac gacgcgacga atggagatct gaaagtcgcc
      601 cactgtgttg atatcgcctg tacgtcggcc acgcttaaga cgctcgacca tgctggggac
      661 gtcggtcagt acggttcttt gaccatcggc atcgacaagc ttccgctcat cacctactac
      721 gatggcacga acggcgacct gaagattgcg cactgcaccg atgtggcctg tgaggccgcg
      781 acgattaccg ttctcacgtt tctgcaggca gaggagaacg tcggccaata tacctcggtc
      841 accatcgccg gagggcaggg tctcatcagc tattacgacg cgaccgatcg ctacctgaaa
      901 tcggcgctct gtcctgattt gtcctgtacg gaaccgactc tcacaacgac tcctgataaa
      961 accggcgatg tgggccgatt cagctcggtt gcgatcggga cggacagtcg ccccctcatc
     1021 agctattacg atgccacgaa cggcgatctc aaagtcatcc attgcgagga ggtcgtgtgc
     1081 gtttccccga cgcgcaccgt tcttgagagc gaaggcaatg tcggccagta cacgtccatc
     1141 gtgatcggag ccgatggcct agccctcatc agctactacg acgtgacgaa cggggatttg
     1201 aaggtggccc actgtggcga tgtcgcctgt acctccgcca cccttgccac gccggacagg
     1261 accggcaacg tcggctcatc cacgtccgtt gccatcggga cagacggtct gcccctcatc
     1321 agttactacg acgtcaccaa cggcgatctg aaggttgctc attgtggcga cgtgagttgc
     1381 accgacgcga ctgttacccc gttggacgga gcagctatta acgtcggcgc ctcctctgcg
     1441 atggtgctgg gagccgacgg caaggcagtg atcagctact acgatgcgac gaacggcgac
     1501 ctcagggtgg ttcactgcgc ggacctcgcc tgcgcatctg ccacgctgac gaccgttcag
     1561 aaccaggaca acgtcggtca ggacagttct gtggctgtcg gcgcagatga gcttcccctc
     1621 atcagttact atgatgcaac gaatggggat ctgcgagtcg cgcactgtac cgatgtcgct
     1681 tgcaccatgg cgaccacatt tacgttagat caggcgggtc aggtcggtca gtttacgtcg
     1741 atgacgatcg gaatggacgg gcgaggtctc atcagctatt acgatgccac gaacggcgat
     1801 ctaaaagtgg cccattgcga tgatgtcgct tgtagctcgg ctaagattac caccttggtc
     1861 agcaagggcg atgtcggtca gttcacctcg accaccgtag gtgccgatgg actggcgctc
     1921 atcagctact atgacatcac gaacgaagac ctgaaaattg cgcattgcga cgatgttgct
     1981 tgtggttctg cgacagaaag cacgttggac agtaggggta atgtcggccg gtatagttct
     2041 gtgacgatcg ggtccgatgg actcgcgttg atcagttact acgatggcac aaacgacgat
     2101 ctgaaagtag ctcactgcat cgacctaacc tgcagctctg ccaaagtgaa tactcttcat
     2161 agcaagggca gcatcggcca attcacgtcc gtcaccattg gtcaagatgg ccgtggcctg
     2221 atcagctatt acgatgccat gaacggggat ttacgagtcg cgcattgcgc caatgttgac
     2281 tgcgaaacgg cgacccacaa tgccgtccag agcttggacg atgtcggtcg atttacgacg
     2341 atcatcacgg ggaaggacgg ccgggctctg atcagctact atgatgtaac gaacggggat
     2401 ttgaaagtgg cgcattgctc caacagcttc tgcgttccct atacccgtca ttgaaacgaa
     2461 tctcttccaa taccactcgg cttgtgcgtg acggctccta cgacgagtaa agatttctga
     2521 tctctaccaa agtcttttcg atttcctgcg gtgagttcag gaggctcggc gcgagcctgg
     2581 catactgcgt tgcgtatggt gtcacgctgc cgacgatgcc gcgctgacga agtttttcca
     2641 cgacggtgcg aggcgccata ccggccacct cgaaacagac gatccccgcc gaaagatcct
     2701 gtgatatcgg tgtgtgcaag gtcacgtgtg gcatggccgc caagccctgc ttcaattgtt
     2761 gattgagttc gtagatgcgc tgggtcacct tggacttccc gacggtttga tgaagcgtga
     2821 aggcttcatc cagcgcccaa cgatgttcga acgagtggaa cccgccgggt gtcatataga
     2881 tggattgcgg aagaccgtgc ggtggtccat ttttcagcca aatctcaaaa gcttgcgaat
     2941 tgaaggttgg gataattgcc tgggccatcg gccaggcatg cgggtggccc caaacgagac
     3001 cggttccacg agggccgaac atccatttgt gagtcccagc gatcaaaaaa tcacagccga
     3061 gttcgctgag gcgaaagtct tcgacaccca acgcgtgaac cccatccaca cagaagatca
     3121 cccggtcctg ctcggctctc gagcgattga tggcctggat cgcttgagcc atttcatgga
     3181 tcggtagttt gagcccggtg ctggaatgga cccaggtaac ggccacgatg cgtgtggcgg
     3241 gggtgatgcc tttgcgcagg gaatcgatga tctcgtctcg ggagacggtc ttgagtgcac
     3301 gatacagggg gatctgccga accttggcgc ctgtacgttc ggcgcgaagg cggagagaag
     3361 tttccgtcga ataatggtcg tggatggtcg tcaagatttc ctgtccggct tgaagccgta
     3421 agccgccgta gaggagaccc agccccatcg tcgtactgtc ggtcaaggca atgtctgtgg
     3481 gctgagcacc cagataatcg gccgccgcct gaagaacctt cgcttcctct ttttcttcgt
     3541 gctcgtacca atagcctata ggatccgagt ccaggccggc gcgatgtcgt tcgatcgctt
     3601 ggcgtaccgg cgtgggatgg gacgcaagga agaacgccgc gagatggatg agttgcggag
     3661 acaatggaaa ctgtgcccgc agatcatccc agcttttgaa cccgctctgc ggaggttgtt
     3721 gatcggcaga agctcgatca tatgcacttg ccagaaccgc tgcgcccaat gccaagccgg
     3781 tacgcacaag gaaacctcga cgtccaatgt cgttcatacg aacctccagg tcaaaactga
     3841 cggcttactc tcctgcttgt ccggaaaggt agacaagaaa cgaccgtgac tattccagcg
     3901 ggctagaaga cagcagcaat aaaggagacg attctgctag gactgatagc tctccatcgg
     3961 cggacaacta caaacgagat ggcggtcccc gtacgcttcg tcgatacggc tcacgctcgg
     4021 ccagaacttg ttgctcctga gccaaggagc aggaaacgcc gccagttccc ggctataggg
     4081 gcgattccac tccgtcgccg tcaccatcgc ggcggtatga ggggcattct tcagcacatt
     4141 gttcgttcgc ggttggcgcc catcgatgat ctcctgaatc tcggcacgga tcaggataag
     4201 tgcttcgctg aaccgatcga gctcgctctt ggcttcactt tccgtcggtt cgatcatgag
     4261 cgtgcctgct accgggaacg aaacggtcgg cgcatggaag ccatagtcca tcaaccgctt
     4321 ggcgacatcc atcgcctcga taccggcgct gtccttgaat tcacgcagat ccaggatgaa
     4381 ctcatgagcc accagtcctg agtccccctt gtacaaaatg gagtaatgct tttccaaccg
     4441 cttggccatg tagttggcat tgaggatggc cacctgtgtg gctttggtca acccgttacg
     4501 acccattaag gcgatgtaca tccatgaaat cggcaggatg ctcggactgc cgaacggagc
     4561 tgccgagaca ggaccgatgg cttgaggcgc accaagcttg accacgggat gccccggaag
     4621 aaacggcacg agatgccgcg ccactccgat cggtcccacg ccaggccctc cgcctccatg
     4681 gggaatacaa aacgtcttat ggaggttgag atggcagaca tcggcgccga tgtctcccag
     4741 acggcagagg cccaccatgg cattcatatt ggccccgtcg atgtagactt gaccgccatg
     4801 ggtatggacg atttggcaaa tgcgccgcac gcccgcctca aatactccgt gggtcgacgg
     4861 atacgtgagc atcagggccg acaatcgatc tcgatactga gctgccttgg cttccagatc
     4921 gtcgatatcc acgtttccgt tccgatcgca cgcgacgacg acaacctcca tgccgaccat
     4981 ggcggcgctg gcggggttcg tgccgtgggc tgagacgggg attaagcaga catctcggtg
     5041 tgtttccccc ttcgaccgat ggtaggctcg gatcaccatc aggccggaat attcgccctg
     5101 ggaccccgca ttcggttgga gggaaaaggc ggcaaatccg gcaatctcag ccagccacga
     5161 ttcgagctgt ccgaacaagg tccgatagcc gagcgtttgt tcacccggcg cgaagggatg
     5221 cagccgagcg aactccggcc aggtcaccgg cagcatttca gcggtggcat tcagtttcat
     5281 cgtgcaggag cccaacggaa tcatcgagtg gacgagggac atgtctttag tctgcaaccg
     5341 gtggagatag cggagcatct cgtgctcgga atgataacga tggaacacct cgtgcgtcag
     5401 atatttactg gttctggcca aaggtgacga gtagctgaaa tcaatgtccg ccgcaagatc
     5461 ggagagacgg aacggcaagt gatcatggcc gacaaaaatc cgtagtaagc gctgtacttc
     5521 ctcctcggag gtgacttcgt cgagcgagac gccgatcgag ccgtcctcat ggtaccggaa
     5581 attgatccgg tgttcgttcg ccctcgttgc tatttgatcc gcttgagcct tgggtaccgg
     5641 tacccgaatt gtatcgaaga cgactttcgg caagacatcg aacccaagtc ggcgcagacc
     5701 ttcacccagc agcaacgtga ggccgtgtac acgctcggcg atccgtcgca acccgtccgg
     5761 cccgtggtac accgcataca tggcggccat gacggccaac aagacttgag ccgtacagat
     5821 gttactggtg gccttctccc gtcgaatatg ttgctccctc gtctgaagcg aaagcctaat
     5881 ggccggtttg ccggtcacat cttttgagat gccgacaagg cgacccggca cctgccgttt
     5941 gtactcctct ttggtagcca aaaacgcggc atgagggcca ccaaagccga tcgggacacc
     6001 gaaccgttgt gtcgaaccga cggcaatatc ggcaccgaat tccccgggcg agcggagcaa
     6061 ggtcaaggcg agaagatcgg tggcaaccgc cactcgaaca tcggcctcat gagcctgcgc
     6121 gaccaatgtg ctgaaatcac ccacgtagcc gtctgtagcc gggtactgca acagaatgcc
     6181 gcacagctga ggactcgaac aatcaacgga ggaggcgaca ccggtcttga tgacgatacc
     6241 caaaggctcg gctctggtct gcaacaccgc caaggtctgg ggatggcagt cgcgcgagac
     6301 aaaaaattcc tttcgttcct cgcccgcgtt gcgggcgatc gtgtaacaca tcgccatcgc
     6361 ttcagctgca gcggtcgctt catccaacag cgaggcattc gccagcggca gaccggtcag
     6421 gtccgccacc atcgtctgga agttcacgag tgcctctaag cggccttgtg aaatctctgc
     6481 ttgatatggg gtgtattgcg tataccatgc aggattttct agaatatttc gctggatcac
     6541 gcccggggtg acgcaatcgt agtaccccat gccgatcagc gatcgataga ctttattttg
     6601 agacgcgatg cctttcaggc gggccaggac ggcctgctcg ccgt
//
LOCUS       DATMTI010000862         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_414708, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000862 DATMTI010000000
VERSION     DATMTI010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414708"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2113)
                     /locus_tag="VN039_13670"
     CDS             complement(<1..>2113)
                     /locus_tag="VN039_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47047.1"
                     /translation="NTDAVYGNSYGAVNFLVMPSDSNFPTLPDSLPGYYGGYSPSGTG
                     ATDAQAVGTGTPADGGSGQFGYGFDSPVENITRACLGIGMGRLSLIGGFVGHETSPDD
                     SVIANTQAMTAVNNWWLSNAKGGVLDPVRTRYQWIAMPGALDFDQLYFWPTGNTGAAW
                     IHLLPINPGSSQSNIWALLEAGTTSGYKLTIRYPDSSTIVETYDNIASDSAMQAAVTG
                     VSAYVNAYVGDGGTPDLTQTVKQMPTTFRDRMVAAVQALYPLGITHFEGPYNEPNCTN
                     NFGKLVPLRMKAFQQAVHDGNAAAKAIGFCPVNISDLDGSNGGSSNWKVFFDAGGGAY
                     LDEISFHTYNCIINGDCNLGRNTMEAMQEFLASYNAGYENLPMWQTEATHAITSVGGV
                     FMPRRARIVPMWWILWQQYGIPHERNPYWYDWSHGFWGYPSMLFTNYAHDKSPNPQAG
                     LLSTLARETFGKAYHHRVDFGSPAANAIFLGSVYGSAAAGSVMTLQTMSYMPSSSVTL
                     TIAGTTNDITLVDSWGNTSTATQSSGRITVDVSDIPTYVRLPAGVNATVYSVNDWGAS
                     PNPNVASAKFTAIMGTRNLPSIADGQFLTRYNALSSMGLNVSNVTTLPDTVELKFYDT
                     DTVTVDRVIVWSGPNYQNLATLVDYDVQTWDGTSWTTQKTVTKTITPSFRFGSNFTNA
                     GCEVETFADEQWIEDVKLPSPV"
BASE COUNT          469 a          611 c          587 g          446 t
ORIGIN      
        1 accggggatg gcaacttcac atcctcgatc cactgttcgt ccgcgaatgt ctcgacctca
       61 cagccggcat tcgtgaagtt agacccaaat ctaaatgaag gagtgatcgt cttcgtgacc
      121 gttttctgcg tcgtccacga cgttccatcc caggtctgga cgtcatagtc aaccagggtc
      181 gcgaggttct ggtagttcgg ccccgaccat acgatcacac gatcaacagt gacagtatcg
      241 gtgtcgtaga acttcaattc gaccgtatcg ggaagtgttg tcacattcga cacgttcagt
      301 cccatcgatg acaaagcgtt gtatctagtg aggaactgtc catcagcaat cgagggcaag
      361 tttctagttc ccatgatcgc cgtaaacttg gctgaagcaa cgtttggatt cggcgaagca
      421 ccccagtcgt taaccgagta aacggttgcg ttcaccccgg cagggagtcg tacatacgtc
      481 ggaatgtcgg acacgtcaac ggtgatccgt cccgagctct gcgtcgcggt cgatgtgttg
      541 ccccacgaat cgacaagggt gatgtcattc gtcgtcccgg cgatcgtcaa cgtcacagac
      601 gatgatggca tgtacgacat cgtttgtaac gtcatcaccg acccggctgc tgctgaaccg
      661 tagacagagc cgaggaagat cgcgttcgcg gctggcgatc cgaaatcgac ccgatggtgg
      721 tacgccttgc cgaatgtctc cctcgcgagg gtagagagaa gaccagcctg cggatttggg
      781 gacttgtcat gcgcgtagtt cgtgaacagc atcgacgggt agccccagaa cccgtgcgac
      841 cagtcatacc aataggggtt tctctcatgg gggatgccgt actgctgcca gagaatccac
      901 cacatcggaa cgatcctggc gcggcgaggc atgaacacgc cgccgactga ggtgatcgcg
      961 tgggtagcct ctgtctgcca catcggcagg ttctcgtagc cggcgttata ggaggcaagg
     1021 aactcctgca tcgcctccat cgtgtttcta cctaggttgc agtcgccgtt gatgatgcag
     1081 ttgtaggtat ggaacgaaat ctcgtctagg taagcgccgc caccggcatc gaagaacacc
     1141 ttccagttac tcgagccgcc gttgcttccg tcaaggtcgc tgatgttgac cgggcagaag
     1201 ccgattgcct tcgcggcggc attgccgtca tggacggcct gctggaacgc cttcatacgt
     1261 aaggggacca atttgccgaa gttgttcgtg cagttcggct cgttgtacgg cccctcgaag
     1321 tgcgttatcc ccaatggata caaagcctgc accgcagcaa ccatacgatc gcgaaacgtc
     1381 gtaggcatct gctttacggt ctgcgtcaag tcaggcgtcc cgccatcccc gacataggca
     1441 ttcacgtaag ccgaaacgcc agtgaccgcc gcctgcatag cagagtcaga tgcgatgttg
     1501 tcgtacgtct cgacaattgt cgaactgtct ggatacctga tcgtcagctt gtaacccgac
     1561 gtcgtgccgg cttccaaaag agcccagatg ttcgactgtg acgagcctgg gtttatcggc
     1621 agaaggtgaa tccaagcagc cccagtgttg ccagtcggcc agaagtaaag ctggtcgaag
     1681 tcaagagcac ctggcatcgc gatccactga tagcgagtac gaaccgggtc aagaactcca
     1741 cccttggcgt tcgacagcca ccaattattc acggcggtca tcgcctgagt attcgcgata
     1801 accgagtcgt cgggcgatgt ctcatgcccc acgaacccgc cgatgagaga caagcgcccc
     1861 atgccaatcc caaggcaggc gcgagtgatg ttctctaccg gactatcgaa tccgtagccg
     1921 aactgaccag accctccatc ggcaggagtt ccagtcccta ccgcctgagc gtcagtcgcc
     1981 cccgtgcccg aaggcgaata accgccgtag taaccaggga gagaatctgg cagcgtcggg
     2041 aagttactgt cactgggcat gaccagaaag ttgaccgcgc cgtagctgtt accatagacc
     2101 gcatccgtat ttg
//
LOCUS       DATMTI010000863         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_626440, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000863 DATMTI010000000
VERSION     DATMTI010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626440"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VN039_13675"
     CDS             <1..1051
                     /locus_tag="VN039_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47048.1"
                     /translation="PMNTLGGKGSAIEEAAQRFCNSGEVHSRKGFRSRLDAAKFHYGL
                     EPDESLYVPETYTYVQRVQKETYAPPTPYHQVKDSAPRSERPVKMGEIEHIMVIPDRH
                     RDPRYPHRLECDTWIAQEGSARRPKYVVDLGDTLTMDSCSRHDKKDTKRGQLRPTIKQ
                     DLDHHVELHQAFERGRAADWKPIKIKCRGNHEERLWDFENQNPEAFGSHTHTYSETLL
                     QFGWKEYPYKQIVYIGGVGFVHAPVNGMGKQMGGKTSSHRAAALLRASLVHGHTHKRG
                     MYDDAKLDPSGPVSVMECGCALPWGEVETYAEGSPIGWWWGIVFLKVCDGAVIDADWV
                     SMATLKERYGQKLAA"
     gene            complement(1580..1936)
                     /locus_tag="VN039_13680"
     CDS             complement(1580..1936)
                     /locus_tag="VN039_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47049.1"
                     /translation="MTDDLLNKVAEAIKHGLLNDGGDAWMDGDNPVNDFVIDGTVNLL
                     SAAQAAIDAMGYQWRPIEEYREGETVLVDTPQLESGCTMAYRQSGAWCSIFDGKPFGR
                     HVTPPKWFTKIPTPPK"
     gene            complement(1933..2334)
                     /locus_tag="VN039_13685"
     CDS             complement(1933..2334)
                     /locus_tag="VN039_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47050.1"
                     /translation="MNKTEAPKTADLRNQIEALVQLNDRGALVPHGIGELARSLLLSA
                     SDTITSLEAEVERLNKCWTKAEDDFDRAVETIDSLKAQLAEADEVIRPFAEAAQIKGV
                     HDMGARSVYPAGPIRVSHVRRASQYLKGREG"
BASE COUNT          582 a          708 c          649 g          585 t
ORIGIN      
        1 accaatgaac acattgggcg gaaaaggatc ggcaattgaa gaagctgcgc aacgtttttg
       61 caattcaggt gaagtccata gccgcaaagg ctttagaagc cgccttgatg ccgcaaaatt
      121 tcattacggc ttagagcctg atgaaagcct ctatgtccct gagacgtaca catatgtaca
      181 gcgtgtacag aaagagacat acgccccgcc gacaccttac catcaggtca aagactcggc
      241 tcctcggtct gaacgccccg taaaaatggg tgagatcgag catatcatgg tgataccaga
      301 tcgccaccgt gatccgcgtt atccgcatcg cctagagtgt gatacctgga ttgcgcagga
      361 gggcagcgcg cgccgcccga aatacgttgt ggatctgggc gacacgctca cgatggacag
      421 ttgctcgcgc cacgacaaga aagacaccaa gcgcggccag cttcgcccga cgatcaagca
      481 agaccttgac catcatgtcg aattgcatca ggcgtttgag cgcggcaggg ctgcggattg
      541 gaagccgatc aagatcaagt gccgaggcaa tcacgaagaa cggctatggg attttgaaaa
      601 ccaaaacccg gaagcattcg gaagccacac gcacacctat tccgagacac tcctgcaatt
      661 cggctggaaa gagtacccat acaagcaaat tgtctatatc ggcggcgtag ggttcgtcca
      721 cgcgcccgta aacggcatgg gtaagcagat gggcggcaag acctcatcgc accgtgcagc
      781 ggcgcttctg cgagcttctt tggtgcatgg acacacgcac aagcgcggca tgtatgacga
      841 cgccaaacta gacccatctg gtcccgtaag cgtaatggaa tgcggctgcg ccctaccgtg
      901 gggcgaggtc gaaacctatg ccgagggttc accgattggc tggtggtggg gcatcgtctt
      961 cctaaaggtc tgtgacgggg ctgtcataga tgctgattgg gtcagcatgg cgactttgaa
     1021 agagagatac ggtcagaaac tcgccgcata gcacaaaacc cccggcgttt aaccgggggc
     1081 tgaacatgcc tgcctacagg cttacggatg gttttgaggt ggtgagtttt gcacttgccg
     1141 attcgggagt tgaacccgtc tggtcaggga cttgcaccct tccgcactca ccgggctact
     1201 accgttcacc ctttcctcaa actcgttacc gccgaagcgg tgaaactgga gcggcaggtt
     1261 tccctttttc acaagcctag acctaggaac caagctatag atagcccaaa cgggaccgca
     1321 taaatatgcc cgaaggcgaa acttgttgcc gtctttccgg ctgtcacccc atccatttct
     1381 ggataccctt acaaggggct ggtttggcgg ggttccctgc attttagtac actggcgcaa
     1441 acggatattt tcacactttg cggtcagttg tcgttgcttt tccgactgcc agaccttttc
     1501 gcggtatcta cgccctttcg ggatcggtta cgccaaaacc ctacccaaaa acacccacaa
     1561 aggcaagcgc tcattcttcc tactttggag gtgtggggat tttggtgaac catttcggcg
     1621 gcgtcacgtg cctgccaaag ggttttccgt caaaaatgct gcaccacgca cctgactgcc
     1681 tatatgccat cgtgcatccg ctttcgagtt gtggggtatc aacgagtacc gtttcaccct
     1741 cccgatattc ctcaatcggc ctccactgat accccatagc gtctatagcg gcttgggcgg
     1801 ctgataacag attcacggtg ccgtcgataa caaagtcatt gacagggttg tctccatcca
     1861 tccaagcgtc gcctccgtca ttcagaaggc catgcttaat cgcctctgct accttattaa
     1921 gaagatcgtc ggtcatcctt cacgtccttt caggtattga gaggcgcggc gaacgtgtga
     1981 aactcgaata ggtccggctg gatagacgct tcgtgccccc atatcatgca cacccttgat
     2041 ttgtgccgct tccgcaaacg gccttatcac ctcatcagct tccgcaagtt gggctttgag
     2101 ggagtcaatc gtttcgaccg ccctatcgaa atcgtcttct gcctttgtcc agcacttgtt
     2161 tagcctctcc acctctgcct ctagggaggt tatggtatca gatgcggaca aaagcaggct
     2221 acgggctaat tctccgatac cgtggggcac aagcgcgccc ctgtcattga gttgcaccaa
     2281 agcttcgatt tgattgcgta aatccgccgt ttttggcgct tctgtcttat tcatgggagg
     2341 agtccttgtc tgtaaacaca ttgcgcgacc tccaaaggtt tcttagttcg ggccaaatca
     2401 ccaatgtgca gttgaagtaa acggctacgc caagaataat ggcgatcaat gctttctcag
     2461 ccatcacccc atctccttta tagcggctcg gcctgcgtca atcgcatccg ctgcggctcg
     2521 taaa
//
LOCUS       DATMTI010000864         4906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_542179, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000864 DATMTI010000000
VERSION     DATMTI010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542179"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..4858
                     /locus_tag="VN039_13690"
     CDS             35..4858
                     /locus_tag="VN039_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47051.1"
                     /translation="MSHRHYTFTRVLSRLAAIALFLAPSALLAEAPSHVAMNHQMPGW
                     AEQLKGQTIIENTLEGQPERTGMVERQHQRVMQQMQHDATVQRTGGYYNDLNMMHQYG
                     AGNQDVLLMSNPGTEPVSMSGGRCPAAAPVRKYDISAINVEISLNMWLDFYPGYMYVL
                     TENLEKVREEEATNRTAREKEGYDPGAVRNGLQNQWIQPLVIRGNQGDCIKMTLRNQL
                     EGGEDVSLNIHGSSMIVASTGKPATTTNPDSIAAKDKAVELEWYIPPTQQEGGRQFHS
                     YSNDRELTVMGLFGTFVIEPKGSQYLDPLGTGEPTPMASGWQAIIKNGAGPDFREFVL
                     IYHEVGDEAFRPLNKKGDFIPQRDPLTDVYRPVARALNYRSEPFGVDNMQTQHEYFGF
                     EDESMAYSAYTFGDPATTVPRSYLGDPAKFRLVHGGSEVFHSHHPHGGSIRWPRSPRA
                     IDEMPLWHAAKNGPVKYPVIRMKSDRVDVEVIGPSETMDLETECGSGLCQQLAGDFLF
                     HCHVAHHYIAGMWGYWRVYNTLQQAEHHNDVMPDLRELPDRAGRIQDGITSDQLIGRT
                     VDWFGKTFTIVKDGKTNWKASPAVVNVKDWVNMMLPPQGQPGHQKDERAQTRSYDASV
                     LDWAWQGDRAMTERENTVDNPRYKSTAPGKRVPILFESATGKVAWPHLKPHFGRRVPF
                     SLNHNPAPWLDMIHLDDDGLPSSYPAKPGENGRWSICPENAGSKKYNVHFIQTPMTLA
                     DKQGDTPAIVDKDGLIYVLHEEEAQVRKSDIKYPLVVRANIYDCIDWMLTSEWLDDDH
                     INFQASKINTHWHFLQFDNQSSDGVITGFSYEQSVRPFTMLEKKVKKGLPVPMNTVFI
                     KAAKKGDRVITVKNAAQYHPNVELLIGADNVEGNEIGRVKSIQGNQITLYRPLKNAHP
                     ANDIVTVEFVRQRFWADSDVGTVFWHDHALGRVTWPHGGFGTLITEPVGSTYHNPKTG
                     KEIRSGPIADIRTAEPVGYGVNGSFRELMVQLNDTVPHTVNIVTAGNPPGQPIEVALE
                     AGKTVSFPMPEKIPMAPMSFLNGGTHTTGGGLNFKAEPISSRLALNPDSSKLFSSAVH
                     GDPYTPMVRAYLGDTVVFRLLQTMANETMVWTVSGHTYLTERYAGDANRKNSIHIGIA
                     ERYDLVVPQAGGPRLQPGDYIHFNGRVSKFSEGAWGIMRVLDKEAPDLQKLPAGYSRR
                     NEIPKPLAICPADAPVKNFNVVALDYPSMKLNPKAPDTIEVDFERTIQMVNPNAKIYA
                     LEEDATKVAGGVQPMPLTLRANVGDCLKVKLTNRMKESRASFSAISLAFDPKDSLGAN
                     VGNNPCDQTIAPGESRTYTYYADPFLGETASLVWDWGNVVTNPRNGLYGAVVIGPKGA
                     QYRDPKTGADISTKNSWVADVIVDRTIQGYEHRVNYRDVALFFQDEDNIIGTSFMPYV
                     QNTAGLTAVNYRAEPYKFREEKGCSLGKVFQPCVVDKPEDPVTPIIEAHAGDPVRIHV
                     FGASNEQNGMFSVERHEWPIEPFMRGADMISVVEFSGSETLDAFIPTAGGPFHMPGDY
                     VWSNQRLPYSQSGQWGYLRVLPAGDQRVLPLSGPGTGAKHASIDEPAHVVPVATR"
BASE COUNT         1128 a         1333 c         1491 g          954 t
ORIGIN      
        1 ttacattttt aatgttatga aggagggtcg gttcatgtct caccgtcatt acacattcac
       61 ccgagtcttg agcagactcg cagccatcgc gctgtttctt gcgccgtcgg ccttgctcgc
      121 tgaggctcca tcgcatgtgg cgatgaacca tcagatgccc ggttgggcgg aacagctgaa
      181 ggggcagacg atcattgaga atacgttgga aggccagccg gagcgtaccg gcatggtcga
      241 gcggcagcat cagcgcgtca tgcaacagat gcagcatgac gcgaccgtgc aacgcacggg
      301 cgggtactac aacgatttga acatgatgca ccagtacggc gccgggaatc aggatgtgct
      361 gctgatgtcg aaccccggca cagaaccggt atccatgtcg ggcggacgct gtccagcagc
      421 tgcgccggtc agaaagtacg acatttcggc gatcaacgtc gagatttcgc tcaacatgtg
      481 gctcgacttc taccccggtt acatgtacgt cctgaccgaa aatcttgaaa aagtgcggga
      541 agaagaagca accaatcgga cggcccgtga gaaggaaggc tatgatcccg gagctgtcag
      601 gaacggattg cagaaccaat ggattcagcc gttggtcatc cgaggcaatc agggcgattg
      661 catcaagatg acgcttcgta accaactgga gggcggcgag gacgtcagcc tcaacattca
      721 cggttccagc atgatcgtcg cctctacggg caaaccggcg acgacgacga atccggacag
      781 cattgcggcc aaagataagg ccgtggagct ggagtggtac attccgccga ctcagcaaga
      841 gggcggtcgg cagttccatt catacagcaa cgatcgcgaa ctgacggtca tgggcctgtt
      901 cggtacattc gtcatcgaac ccaagggatc ccaatatctc gacccgctgg gaaccggcga
      961 gccgacaccg atggccagcg gatggcaggc catcatcaag aacggggccg ggcctgactt
     1021 ccgggaattc gtgttgatct atcatgaagt cggggacgag gcattccggc cgctgaacaa
     1081 gaaaggggat ttcattcctc agcgtgatcc tttgaccgac gtgtatcgac cggtggctcg
     1141 cgcattgaat taccggagcg agcccttcgg cgtcgacaac atgcaaacgc agcatgagta
     1201 cttcggattc gaagacgaat ccatggccta cagcgcctac acgttcggcg atccggcgac
     1261 gaccgtgcct cgcagctatt tgggggatcc ggcaaagttc cgcctcgtgc acggcgggtc
     1321 ggaggtgttt cactcccacc atccgcacgg tggatcgatt cgctggccgc ggagtccacg
     1381 cgccatcgac gaaatgcctt tgtggcatgc ggccaagaac gggccggtca aatatcccgt
     1441 gattcggatg aagtccgacc gcgtggacgt ggaagtcatc ggaccgtccg agaccatgga
     1501 cctggaaacc gaatgtggat ccggcctttg tcagcaattg gccggggact tcctcttcca
     1561 ttgccacgtc gctcaccatt atattgcggg gatgtggggc tattggaggg tgtacaacac
     1621 gctgcagcag gcggagcatc acaacgatgt gatgccggat ctgcgcgagc tgccggaccg
     1681 ggcgggccgt attcaagacg gtattacatc ggatcagctc atcggccgca ccgtggactg
     1741 gtttggaaag accttcacca tcgtcaagga cggaaaaaca aactggaagg cgtcacctgc
     1801 cgtcgtcaac gtgaaggatt gggtcaacat gatgctaccg ccgcagggac agcctgggca
     1861 tcagaaagat gagcgcgccc agacccggtc ttacgacgca agcgtgctgg attgggcttg
     1921 gcagggtgat cgcgcgatga ccgaacgcga aaacaccgta gacaatccga ggtacaaatc
     1981 gacagctccc ggcaagcggg tgccgattct ctttgaaagc gccaccggca aggtcgcctg
     2041 gccgcatttg aagccgcatt tcggccgtcg ggtgcccttc tccctgaatc acaatccagc
     2101 tccatggctg gatatgatcc acctcgacga cgacggtctg cccagctcct atccggcaaa
     2161 accgggagag aatggacgct ggagcatctg ccccgagaac gcgggctcca agaaatacaa
     2221 cgtgcatttc attcagacgc cgatgacgtt ggccgacaaa cagggtgata ctccggccat
     2281 cgtagacaag gacggactga tctacgtgct gcatgaggaa gaagcgcagg tccgcaagag
     2341 cgacattaaa tatccgctgg tggtacgcgc taatatttat gattgtatcg actggatgtt
     2401 gaccagcgag tggttggacg acgaccatat caatttccag gcatcgaaga tcaatacgca
     2461 ctggcacttc ctgcagttcg acaatcaatc gtccgacggc gtcatcaccg gcttttcata
     2521 tgagcaatcg gtgcgtccgt tcacgatgct ggagaagaaa gtgaaaaagg gcctgcctgt
     2581 gccgatgaat accgttttca tcaaggcggc gaagaagggc gaccgggtca ttacagtcaa
     2641 gaatgccgct caataccatc cgaacgtgga actcctgatt ggagcggaca atgtcgaagg
     2701 caacgagatc ggtcgggtca agtcgatcca aggcaatcag atcacgctgt atcgtccgct
     2761 gaaaaatgct catccggcca acgatatcgt gacggtggag ttcgtccggc agcgattctg
     2821 ggcggattcc gacgtgggaa cggtcttctg gcacgaccat gcgctggggc gtgtcacgtg
     2881 gcctcacggg ggcttcggca cccttatcac cgagccggtc ggttcgacct accataatcc
     2941 taagaccggc aaggagatcc ggagcggacc gattgccgac atccgaacgg cggagccggt
     3001 cgggtatggg gtgaacggca gcttccggga gttgatggtg cagctcaatg acaccgtgcc
     3061 gcatacggtg aacatcgtta cggccggtaa tcctccgggg caaccgatcg aagtggcgtt
     3121 ggaagccggg aaaacggtat ccttcccgat gccggaaaaa attcccatgg cgccgatgtc
     3181 tttcctcaac ggcggaacgc atacgacggg tggcggattg aatttcaagg ccgagcccat
     3241 ctcgagtcgc ctggccttga atccagattc gtccaagttg ttcagcagtg ccgtgcacgg
     3301 cgacccctat acgccgatgg tacgggcgta tctgggcgat acggtggtgt tccggctgct
     3361 tcaaaccatg gcgaacgaga cgatggtatg gacggtttca ggccatacct atctgaccga
     3421 acgctatgcg ggcgatgcga atagaaagaa ctcgatccat atcggcattg ccgaacggta
     3481 cgatctggtg gtgccgcaag cgggtggacc gcggctgcaa ccgggagact atatccactt
     3541 caacggccgc gtgtcgaagt tctcggaggg tgcctggggt atcatgcggg tgttggacaa
     3601 ggaggcacca gacctccaaa agctccccgc cggatacagc cggcgaaatg agattccaaa
     3661 accgctggcg atctgtccgg cggatgctcc ggtgaaaaac ttcaacgtcg tggcattgga
     3721 ttatccttcg atgaagttga accccaaggc gcctgacacc atcgaggtcg acttcgaacg
     3781 gacgatccag atggtcaatc cgaacgccaa gatttacgcg ttggaagaag atgccacgaa
     3841 ggtcgccggg ggtgtccagc cgatgccctt gaccctgcgg gctaatgtcg gcgattgcct
     3901 taaagtgaag ctgaccaacc ggatgaagga gagccgagct tcattctctg ccatcagttt
     3961 ggcgttcgac cccaaggatt cgctcggtgc gaatgtcggg aacaatccct gtgatcagac
     4021 catcgcgccg ggagaaagcc gcacctacac ctattatgcg gacccattcc tgggagagac
     4081 ggcctcattg gtgtgggatt ggggcaacgt ggtgaccaat cctcggaacg ggctctatgg
     4141 agcagtggtg atcggcccga agggcgccca gtaccgtgac ccgaaaaccg gtgcggatat
     4201 ttcgacgaag aacagctggg tcgccgatgt catcgtcgat cgaacgattc agggttatga
     4261 gcatcgcgtg aattatcgtg atgtggctct gttcttccag gatgaggaca atatcatcgg
     4321 gacgagcttc atgccctatg tacaaaacac ggcgggtttg acggcggtca actaccgagc
     4381 ggagccgtat aaattccgcg aagagaaggg gtgcagcctt ggcaaggtgt tccaaccctg
     4441 tgtcgtggac aagccggaag atccggtgac gccgatcatt gaggcgcatg ccggggaccc
     4501 ggttcgaatc catgtgttcg gcgcgagcaa tgaacagaac ggcatgttca gtgtcgaacg
     4561 tcatgaatgg ccgattgagc cgttcatgcg cggagccgac atgatcagcg tggtggaatt
     4621 ctccggatcg gagacgctgg atgcgttcat cccgaccgcc ggagggccgt tccacatgcc
     4681 gggcgattat gtctggagta atcagcggtt accctactcg cagtctggac agtggggcta
     4741 tctgcgggta ttgccggctg gagatcaacg agtattgccg ctctccggtc ccgggacagg
     4801 agcgaagcat gcttccatcg acgaaccggc acatgtcgtt ccggtagcaa cccggtaact
     4861 cgattgcctc tgaggagagg taatcgcaat atcgaagagg gggagc
//
LOCUS       DATMTI010000865         6478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_499896, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000865 DATMTI010000000
VERSION     DATMTI010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499896"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VN039_13695"
     CDS             <1..660
                     /locus_tag="VN039_13695"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HWV47052.1"
                     /translation="VMKVAIRAANGKRVCEASLYDASLVLLYEERCSMKVNVGFWIGC
                     ALLSIVVSGCNRYQVIPDHLVKQVNEKLSYEQVKDSPETYRGQIVVWGGEVVSAVRED
                     NRTKIEVLQMPLNRNQVPESSKASSQGRFLAVDSRQEIIDPAIFEKGSKVTVIGEIDG
                     KSPKSSDLVSSESPVLVIRDMTLWSNDSMLYPVRPYYGYGGYYGYRPYVFWEGTRVSG
                     S"
     gene            748..2469
                     /locus_tag="VN039_13700"
     CDS             748..2469
                     /locus_tag="VN039_13700"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HWV47053.1"
                     /translation="MRYVAIATDYDNTLAVDGRVERKTIDALERLIQSGRKLILVTGR
                     LLPDLLDIFPAIGLCERVVVDNGAVLYRPATREHTLLAPPIPAAFVDELRRRQVPELS
                     FGDSIVGTVRPHELTLLEIIRDLGLELQVIFNRDAVMVVPSGVNKASGLAAALQEMGL
                     SSRNVVAIGDAENDHALLNQCEYAVAVANAVPMLKEIADWVTTGAAGRGVVQVIDELV
                     AHDLSVSPFSASRRNILIGMRETGEEVSIPATGLNLLLAGSSGSGKSTLATGILERVT
                     EQGYQFCVIDPEGDYEGFAGAVMFGTSQRGPGMTEILTALESPDTNLVVNLVGLPLQD
                     RPAFFLALLSALQERRAKSGRPHCILVDETHHLLPRDWQPVQAVLAKDLTGMMYVTVH
                     PDHVAKSVLHTVDVIVALGDDPAGTIAGYCGTLGLPLPEVEPAALDHGEAVFWDRRST
                     ELPCTVRIAPCEADRKRHRRKYAEGELPENRSFFFRGPDNLLNLRAHNLILFMHLAEG
                     VDEKTWLHHLRRGDYSKWMSTGIKDSHLADAVRLIEQQPSVNAERSRQMIRSAIEERY
                     TYPATGM"
     gene            complement(2524..2970)
                     /locus_tag="VN039_13705"
     CDS             complement(2524..2970)
                     /locus_tag="VN039_13705"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HWV47054.1"
                     /translation="METVLRALIIYLVLLFVFRLAGRRTLMQMTSFDFVLLLIISEAC
                     QNAMLGNDYSITAGVLAIVTLVGADILLSYWKHRSATAERWLDGLPMIIVENGIPLKP
                     LMDRARIDEEDVLAAARQGPGLERMDQIKYAVLEVGGGISVIPKHS"
     gene            complement(3005..3520)
                     /locus_tag="VN039_13710"
     CDS             complement(3005..3520)
                     /locus_tag="VN039_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47055.1"
                     /translation="MASSPVRIRDLEISQDLDLQRTTWTLQRIGWGGMALIILAALSG
                     VFGSGPLARTEVTDDPHTVRLLYDRFGRYEDSLVLQLVLAPETIKTNRVTVEVDRAYW
                     TSHAVEHITPEPLVSSIGIDGFLYTFEADARSAPAVIVFHLRPKYVGALDGRIRVNDS
                     GPLRFHQFMFP"
     gene            complement(3569..4003)
                     /locus_tag="VN039_13715"
     CDS             complement(3569..4003)
                     /locus_tag="VN039_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV47056.1"
                     /translation="MEGYGKRALIIDDNEDILHLTRMTLMDRGYNVYTASDGFGGSEQ
                     MKKRRYDVVLVDYHMPRLNGPQFIEMSRIMWPETPIILMSVDNYVTDHSDLVRGIFGS
                     IAKQFEPHRLIELVNQACRTLSQRDLFNLPCDGRTAAQAEQY"
     gene            complement(4093..5550)
                     /locus_tag="VN039_13720"
     CDS             complement(4093..5550)
                     /locus_tag="VN039_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV47057.1"
                     /translation="MDRPTILIVDDDAETQALLRDALSKEEYGVDTAADGKIALDMVT
                     RLGPDLVISDINMPGLNGLALLEELRSRGHDMPVILMTAYGSLKTAVDGIRGGAFDYL
                     SKPFIMDDVRLVIHRALEHTQLSRQNQQLKEQLRDRYRFDNLVGSSPALVSVYKSIAR
                     VAQTDSTVLLEGESGTGKELIARAIHANSTRSAGPFVTVDGGALTETLLESELFGHER
                     GAFTGAVGTKKGLLEKAHLGTCFLDEVADLSPALQGKLLRVIQEKEFRRVGSTMTTTV
                     DVRIIAASKKDLSSLVQAGTFREDLYYRLNVVTIHIPPLRERMEDVPLLAQHFVQTYG
                     SSKPKPVTGMSAEAIAVLTHYWWPGNVRELEHAIERAIALTPHPIIYPEDLPHAVHTA
                     TVQTAAQAQGWVTLEELEREHIVRVLKHHHHDLGRSAAILGIHRKTLLRKLRQYGLAD
                     GQRTRYLPPDILSTPVRSGPSPAISNPQELDPSRS"
BASE COUNT         1420 a         1759 c         1852 g         1447 t
ORIGIN      
        1 gttatgaagg tggcaatccg agcagcaaac ggtaaaaggg tgtgcgaggc gtctctttac
       61 gacgcctcgc ttgtgctgct ctacgaggag aggtgttcta tgaaagtcaa tgttgggttc
      121 tggatcggat gtgcgcttct ctcgattgtc gtgagcggat gtaaccggta tcaggtcatc
      181 ccggatcatc tggtgaaaca agtcaacgaa aagttgtcgt atgagcaggt caaagattcg
      241 ccggaaacct atcgagggca gattgtcgtg tggggcggag aagtggtcag tgccgtccgc
      301 gaagacaatc ggacgaaaat cgaggtgcta caaatgccgt tgaacaggaa tcaggttcct
      361 gagtccagca aagcgtcatc gcagggacga tttttggcag tggacagtcg gcaggagatc
      421 atcgatccgg caatttttga aaagggatcg aaagtcaccg tcatcggcga gattgacggg
      481 aaatccccaa aatcgtccga cctcgtgtca tctgagtcgc ctgtcttggt cattcgcgac
      541 atgacgctgt ggagtaacga ttcaatgctc tatccggtcc gcccttacta cgggtatgga
      601 gggtactatg gttatcggcc gtatgttttc tgggaaggga ctcgtgtgtc gggaagttaa
      661 gcccgctgag acccggtggg atgccgtatt ttcgatccat gatagctcta tcaagacgaa
      721 gggcacttgt cattcggcgt acatcctatg agatatgtcg ccatagcgac ggattacgat
      781 aacacgctgg ccgtcgatgg ccgggtggaa cgcaagacga tcgacgctct tgagcgcctc
      841 attcaatcgg gacggaagct cattttggtc acagggcgtc tgctgccgga tctcttggac
      901 atctttcccg caatcggtct gtgcgaacga gtcgtcgtag acaacggcgc tgtgctctac
      961 cgtcccgcga cacgcgaaca cacgctgttg gcgccgccga ttcccgcggc attcgtcgac
     1021 gaactgcgcc gccgacaggt cccggagctt tcgttcggag attccatcgt gggcacggtg
     1081 cggccgcatg agctgacgct gctcgagatc attcgagatc ttgggctcga actacaagtc
     1141 attttcaacc gggacgccgt catggttgtt ccgtccggag tgaacaaggc cagtggtctt
     1201 gcggcggcat tgcaagagat gggcttatcg tcgcgcaacg tcgtggcgat cggagatgcg
     1261 gaaaatgatc atgccttgct caaccagtgt gaatatgccg tagcggtagc gaatgccgtg
     1321 ccgatgctca aggaaatcgc cgattgggtc accaccggtg cggctgggcg gggagtcgtc
     1381 caagtgatcg atgaacttgt ggcgcatgat ctctctgtgt ctccgttcag cgcgagccgt
     1441 cgcaatattc tgatcggtat gcgcgagacc ggtgaggagg tgtcgattcc cgccacagga
     1501 ctcaacttat tgctggcggg ttcgtcggga agcggcaaat cgacgttggc caccgggatt
     1561 cttgagcggg tgacagaaca agggtatcag ttttgtgtga ttgatcctga aggagactat
     1621 gaggggtttg cgggagcggt catgttcggg acttcgcaac gggggccggg aatgacggag
     1681 attctcaccg ctctggaaag cccggacacg aatttggtcg tcaatcttgt gggcttgccg
     1741 ttacaggatc gtccggcatt ttttctggcc ctcttgtcgg cccttcagga gcgtcgggcc
     1801 aaatccggtc gaccgcattg tatcctcgtc gacgaaacgc atcatttact gcctcgtgac
     1861 tggcaaccgg tgcaagctgt gctggcgaaa gatttgaccg gtatgatgta tgtcaccgtc
     1921 catcccgacc atgtcgcgaa atccgtgctt cacacggtag atgtcatagt ggccttagga
     1981 gatgacccgg cgggtacgat tgcgggatat tgcggaacgc tgggcctgcc gttgccggag
     2041 gtcgaacctg cggcgctgga tcacggtgaa gcggtctttt gggatcggcg gtccaccgag
     2101 ttgccctgca ccgtgcgcat cgccccttgt gaagcggacc gtaagcgtca ccgccggaaa
     2161 tacgcggaag gcgaattgcc ggaaaaccga agcttttttt tccgtggtcc ggacaatctg
     2221 ctgaacttac gagcgcacaa cctcattctc ttcatgcatc tcgcggaggg tgtcgatgag
     2281 aagacgtggt tgcatcacct tcggcggggg gattatagca aatggatgtc gacgggtatt
     2341 aaagattccc atttggccga tgccgttcgg ttgattgaac aacagccctc cgtcaacgcc
     2401 gaacgcagcc gacagatgat acgttccgcc attgaggaac ggtacacgta tcccgcgacc
     2461 ggcatgtgat gcgcaaggcg gttcgctgac acgtgagatc gacctgccac gccctcatca
     2521 cattcacgaa tgtttaggaa tgaccgaaat gcccccgccc acctccaaga cggcatattt
     2581 gatttgatcc atacgctcga gccccgggcc ttgcctggcg gccgccagca catcttcctc
     2641 gtcaattctc gcacggtcca tcaacggctt cagtggaatg ccgttttcaa cgatgatcat
     2701 cggcaggccg tccaaccacc gttccgccgt cgctgagcga tgtttccaat acgagagcag
     2761 aatatcggcc ccgacgagag tcacgatggc caacacgccg gccgtgatgg agtagtcgtt
     2821 gcccagcatg gcgttctggc aggcttcgct gatgatcagc agcaagacga aatcaaagct
     2881 cgtcatctgc atgagggtgc ggcgaccggc cagccgaaag acgaacaata acacgagata
     2941 gatgatcagc gcccgcagta ccgtttccat acggaacctc cctggttaca ctcgatgccc
     3001 gatcctaggg aaacatgaat tgatggaatc ggagcggtcc ggaatcgttg acacggatgc
     3061 gaccgtccag cgcaccgacg tatttcggcc gcagatggaa gacgatgacc gccggcgcag
     3121 accgtgcgtc ggcttcaaat gtataaagga atccgtcgat gccgatactt gatacgagag
     3181 gttcgggagt gatgtgttcg acggcatggc tggtccagta cgcccgatcg acctcgacgg
     3241 tcactcggtt ggtctttatc gtttcagggg ccagcacgag ttgcaagacc aatgagtctt
     3301 catagcgccc aaagcgatcg tacaacagcc gaacggtgtg cggatcgtcc gtaacttccg
     3361 tgcgggccaa gggtccgctt ccgaacacac cggacaacgc cgccaggatg atcagcgcca
     3421 tgccgcccca accgatgcgc tgtaaggtcc aggttgttcg ctgtaggtcg aggtcttggc
     3481 tgatttcaag atcgcggatt cgtacaggag aagacgccat aacggtttat gacctcaact
     3541 tactcgtcgg atctccgcac gatcgcaatc aatattgttc cgcctgggcg gccgttctgc
     3601 cgtcgcaagg cagattgaat aggtctcttt gggaaagggt gcgacaagcc tgattcacga
     3661 gttcaattaa tcgatgaggt tcaaattgtt tagcgatact cccgaagatc cctctgacaa
     3721 ggtcggaatg atcggtcaca tagttgtcca ccgacatcag aatgatgggg gtctcaggcc
     3781 acatgatgcg ggacatttcg ataaattgcg ggccgttcag ccgcggcatg tggtagtcca
     3841 ccagcacaac gtcataacgt cttttcttca tttgctcaga ccctccgaag ccgtcggaag
     3901 ccgtatacac gttatagccg cgatccatca gcgtcatcct ggtcagatgc agaatgtcct
     3961 cattgtcgtc tatgatcagt gcgcgtttgc cataaccttc catctcacca ccctccgcgt
     4021 acgatcatcc gtgaagtcga agtacatcca actcataccg gctgaagaaa ccggcaaaga
     4081 acagtccacg acctaagacc gtgatgggtc caattcctgt ggattggaga tggcgggaga
     4141 cggcccactc cggaccggcg tagacaagat gtccggaggt agataccttg tccgctgccc
     4201 atcggcaaga ccgtattgcc gtagtttccg caacaacgtc tttcggtgaa tccccaaaat
     4261 cgcggcggac cgccccagat cgtggtgatg gtgcttcaag acccgtacga tatgctctcg
     4321 ctctaattcc tccaaggtga cccatccttg tgcttgagcc gccgtctgca ccgtggccgt
     4381 atgcacggca tgtggaagat cctcgggata aataatggga tgcggagtaa gggctatcgc
     4441 ccgctcaatg gcgtgttcga gttcccgcac gtttcccggc caccagtagt gggtcagaac
     4501 ggcgatggct tcagcggaca ttcccgtgac cggcttcggt ttggatgaac cataggtctg
     4561 cacaaaatgc tgtgccaaga gcggcacgtc ttccatacgt tcccgcaacg gcgggatatg
     4621 tatcgtcacc acattcaatc gataatacag gtcttcacga aacgttccgg cctgcacgag
     4681 tgaactgagg tcttttttcg acgcggcaat aatgcgtacg tcgacggttg tggtcatggt
     4741 actgccgacg cgtcggaatt ctttctcttg aatgacacga agcaatttcc cttgcagcgc
     4801 cggcgacaga tcggccactt cgtcgaggaa acatgtgccg agatgggctt tctccaatag
     4861 accctttttg gtgcccacgg cgccggtgaa agcgcctcgt tcgtgcccga acagttcgga
     4921 ttcgagcaga gtttcggtca atgctccacc atcgactgtc acgaaggggc cggcactccg
     4981 tgtgctgttg gcatgaatgg cccgggcgat gagttcttta ccggttccgc tttcaccctc
     5041 cagcaaaacc gtgctgtctg tctgagcaac ccgagcgatg gatttgtaaa cggaaacgag
     5101 ggccgggctg ctgccgacta gattatcaaa ccggtaacga tcgcgaagct gctctttgag
     5161 ctgctggttt tgcctggata gttgagtatg ttcgagcgct cgatggatca ccaaccgcac
     5221 atcgtccatg atgaacggtt tactgagata gtcgaacgcc cctcctcgga tgccgtccac
     5281 cgccgttttc aggctaccgt aggccgtcat aagaatgacg ggcatgtcgt gtccgcgaga
     5341 acgtaactcc tcgagtagag cgaggccgtt gagtccgggc atattgatat cggatatcac
     5401 gaggtctggc cccagccgag tgaccatatc cagggcgatc tttccgtcgg ccgcggtgtc
     5461 gacgccgtac tcttctttcg acaaagcatc tcggagcaac gcctgtgttt cggcgtcatc
     5521 atccacgatc aaaatagtgg gacggtccat gtcatcaccc cgttatggca acggccggcc
     5581 tggccgcgat cttcgcacat gtcggtccct gggcatcatt cggacacatc cgaaggctgt
     5641 gcgcagcgtc atcgatgcgc cgatcaagac ggctcatgac aggtcgtcga taagcaggaa
     5701 caagttgtcc cattcgtcgg tgcggatctg cagccaagcg gcaagctcga aacgctgaat
     5761 tgtcaacaac ttcgttcgac gcatctgcct ggcagtgttc gtgcttagtc catggcaaca
     5821 gatacatgct cgactcgatc atggggcggg ccgcttcaca atcaacaaaa gaggtgctgc
     5881 catggtttgt gaacgatgcg gtggacttaa agtacatgat tatttctacg gcgccaccga
     5941 gtactttgcg tggcaatgtt tgggctcccg ttgcgtgaat tgcggagcga tcagtaacat
     6001 tcaacccatc gattccagta aaccaagctc aaggcgcgac acgaatcatt cacgaagacg
     6061 acaagacact gtagcacggt ggcattcgtg aacacgtccg agcgcgcgta accacatgtc
     6121 attcaccgcc accacccttt tacgtctggt ggggcggtga cggcaccagg cgatcagaac
     6181 accggccttg aataaccatg agcacaaatt gtcacgaccg gatgtgtcac gcatggcttt
     6241 cttgcaccga tcttttaggc ggtagtgtga tccaagacgc aatgggtgcc cactggagat
     6301 accccttggt gcttcatcgt atgaagtgcg cggatcgacg gccgctagac agaccccaag
     6361 cactgcggcg tgaagcacat gcatacagaa ataactctgc ctctcgcatc catggttgcc
     6421 tagaggcttt cccagtatcc gaatgctatg aagtcagtgt agacagaggt ccatgtga
//
LOCUS       DATMTI010000866         1640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_840108, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000866 DATMTI010000000
VERSION     DATMTI010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840108"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VN039_13725"
     CDS             <1..627
                     /locus_tag="VN039_13725"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV47058.1"
                     /translation="AIKKDRSFLPAYIGMGGILLHEGKTKDAVEILKKVYSRTRSVII
                     LHRLEELFLDQGEPSEIIRVYQEALQQDPHNPILQFYLGKLYYRLEMVDEAFDLLSTI
                     EGPQDHLLDYHKIMANLYLRKQHFEEAIVELKKALSFKKRVVVPYICTQCQQESIEWT
                     GRCRRCAKWNTLTALPWLEAGHTTTPAGEPSSVRAIPYQGIASPFETV"
     gene            739..>1640
                     /gene="bioB"
                     /locus_tag="VN039_13730"
     CDS             739..>1640
                     /gene="bioB"
                     /locus_tag="VN039_13730"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWV47059.1"
                     /translation="MTYMTLAEKALNDEPLTRVECRSVLTTPDDELLALLHAAFQVRS
                     KYFGRTVRLQMLQNAKSGACQEDCHYCSQSSISTAPIERYNLLPQKQMIEGARQAAAS
                     KAQRYCIVISGRSPLDREIDEIAGAVRSIKQEIPIQICCSLGLMNESQAKRLKAAGVD
                     RVNHNLNTSEAFHSSICTTHTFQDRLATIQNARTAGLEICSGGIVGMGESEEDVIELA
                     MALRDVKPDSIPLNTLHPVAGTPLENCDSLTPQRCLKVLCLFRFVHPRTEIRVAGGRE
                     HNLRSLQPLALYPADSVFVNGYLTT"
BASE COUNT          386 a          471 c          410 g          373 t
ORIGIN      
        1 gctatcaaga aggaccgaag ctttctcccc gcctatatcg ggatgggcgg aattctgctc
       61 cacgaaggca aaaccaaaga tgccgtcgaa atcctcaaga aggtctattc acggacgcga
      121 agcgtgatca ttctgcatcg actggaagaa cttttcttgg atcaaggcga gcctagtgaa
      181 atcattcgag tctatcagga ggccttacag caagatccgc acaatccgat cctccagttc
      241 tacctaggaa agctctacta tcggctggaa atggtggatg aagcgttcga cctgctgtcg
      301 acgatcgaag gcccgcagga ccatctgctg gactaccaca agatcatggc caacctgtac
      361 ttgcggaagc agcattttga agaggctatc gttgaattga agaaggccct cagtttcaag
      421 aagcgcgtag tcgtccctta tatctgcaca cagtgtcaac aggaatccat tgagtggacg
      481 ggccgctgcc gccgctgcgc caagtggaac acgctcaccg cccttccctg gcttgaagcc
      541 ggtcacacta ccaccccggc tggagagccc agctccgtcc gtgctattcc ctatcaaggc
      601 attgcttctc cgtttgaaac cgtgtaggtt cgcgccagcc tctgttgatt gttcggtcta
      661 ccggtttgtg gagacctgct cctcgattcg acaaatttga agtacagcag attaccacgt
      721 cattactttg aggattccat gacctatatg actttggcag aaaaagcgct caacgacgaa
      781 ccccttacca gagtagagtg ccgttccgtc ttgacgacgc ccgatgacga attgctcgcg
      841 ttgttgcatg cagcctttca agtccgctcc aaatacttcg gtcggaccgt ccgcctccag
      901 atgttgcaga atgccaagag cggagcctgc caagaagact gtcactactg ttctcaatct
      961 tccatctcga cggcgccgat tgagcgatac aacctgcttc cccagaagca gatgatcgaa
     1021 ggtgcgcgac aagctgccgc ctcaaaagcc cagcgttatt gcattgtcat cagtgggcga
     1081 agccctttgg atcgggaaat cgacgaaatc gccggagccg ttcgttcgat caagcaggag
     1141 attccgatcc agatctgttg ctccctcggc ctcatgaatg agtctcaggc caaacgactg
     1201 aaagccgcag gtgtcgatcg cgtgaatcac aacctgaata ccagcgaagc ctttcactca
     1261 tcgatctgca ccacccacac cttccaagat cggctcgcga cgattcagaa tgcccgaaca
     1321 gctggactag aaatctgttc aggtggcatt gttggcatgg gcgagagcga ggaggatgtc
     1381 attgaactag cgatggctct gcgcgacgtg aaaccggatt ccattccctt gaatacgctg
     1441 catccggttg ccggtacacc gttggaaaat tgtgattccc tcacacctca acgctgtttg
     1501 aaagtgctct gcctcttccg gtttgtgcac cctcgaacgg agattcgcgt cgcgggcggc
     1561 cgcgaacata atctgcgcag tttacaaccc ctcgccctct accctgctga ttcagtcttc
     1621 gtgaatggct atctgacgac
//
LOCUS       DATMTI010000867         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_710528, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000867 DATMTI010000000
VERSION     DATMTI010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710528"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1169
                     /locus_tag="VN039_13735"
     CDS             99..1169
                     /locus_tag="VN039_13735"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV47060.1"
                     /translation="MKTPLALVLVICAAIGSTAALADDAPDATNASVLVNLVPLKKGS
                     VPVSITVYGRVMPAEKGRESLSSPIAARVSNINVHVGQEVDRGTPMITLTPSPESRAA
                     YKQARQAVLTANHLLDRSRSLANAHLETAAQLALDEKTAADANSTLEALEEEGANGTN
                     TLKAPFKGIVMSVSVSSGAVVAQGTSLIELANSKGLVFKTGVVPAQAARIKNGDAVTM
                     TPFSGSGTLHGRVSLRGSAVNLENGLVPVDVTISDGNPLTGEMARAVINTGEQQGYVV
                     PHDAILLNDSGQTYVVQSVNLVAKKVPVTVMGSAGDTDVVNGKLVAGAPIVGAGSYQL
                     DDGDKMRLTNSTTSASGGKAGQ"
     gene            1166..>4093
                     /locus_tag="VN039_13740"
     CDS             1166..>4093
                     /locus_tag="VN039_13740"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV47061.1"
                     /translation="MNFIDWVERHARSLIFIAFALTLAGIFAALTLPIQLFPTVSFPR
                     VRIEVDSGDMPAKQMLIDVTEPLEKVSRAVPGAVNVTSTTTRGSAEIFVDFPWGSDMH
                     RAVLNVDAAFAQKLPDLPQGTGYDVIQMSPNAIMPFVSYALISDKISPADLKTLAKYQ
                     IAPLLTGIPGIRQVSTLGGETREVQVYVSPEKLQTYGLTLTNLSDAISGTNTLEAVGR
                     IQDNGLLYLTIENNQFSSIEAVRDVTLHTSNGGVVRLGDIAKVEMGSVPQWLLVDDNG
                     QPSVNINVFQQNTADTLTLEKEVQAKLDAFMKTQPKSIQIHKWYDQAELVRSSIGAVE
                     EAIVIGLILAACVILGFLRNWRAAFVAMITVPMSVMTTCVLLSALGLSFNIMTLGGIA
                     AAIGLLIDDAIVMVEQIARRAGVPGLEHPQRTVLSSAREFLSPLTGSSSATIIMFIPL
                     AFLSGVTGAFFKSLSLTMAASLIISYGFTALLVPLMARGIIDFKKWHDPSHGRETWSR
                     RTHGRVLTRLFAAPWLTAIILVVLAGVGYLGYTHVGTGFLPRMDEGGFVLDYQTKPGT
                     SLIETNRELAQVEAILQKNSYVDTYTRRTGAGLGGDLNLPYQGDFFVHLVPPSKRPPI
                     WQVMNSLSAQINSQVPGIDYDTHQLLSDMIGDMVGRRQPVVVNLSAKDPDVLPGVADK
                     VADALKKVDGIEPSSVDPGTLPTGDALEITVDQAQAAMNHLTASDVKDQIYRYLHGSV
                     ITKYMGRVQDVGVRLWVQPQNTGDRENTRLYRDDLYKLLIQAPNGHTLPLGAIAKIKF
                     VSGQPALFRANLAQIVSLTAQTNGKRDLGSTIAAVKQILDKPGTVPSGVYYTLGGAYK
                     QQQEAAHGMVITFSAVVLAEIILLLFLYERLSLPLIILFTSLVSTGAVFTGLWLTGTE
                     LNITAMMGMVMILGIATEMAIFLVSEYQMLEKEMPPREAIYQAALNRFRPILMSTLAM
                     ILAL"
BASE COUNT          825 a         1188 c         1197 g          883 t
ORIGIN      
        1 cagcggacgc tcgacgaaac cgctcttgcg ctgtcgatag agctcggcgc cggctttccc
       61 aaaacaatgc ccgtttctcc aagcaaagtg acaaacccat gaaaacgcct ctcgccctcg
      121 ttctcgtcat ttgcgctgcc atcggctcga cggccgcgct cgccgatgac gcccctgacg
      181 cgaccaacgc cagcgtgctc gtcaacctgg ttccgctgaa gaaaggcagc gttcctgtct
      241 ccattaccgt ttatggccgg gtgatgcccg cggaaaaagg gcgggaaagc ctctcttcgc
      301 cgatcgctgc ccgggtgagc aatatcaatg ttcacgtcgg gcaggaagtt gacagaggta
      361 cgccgatgat cacgctcacc ccgagcccgg agtccagagc tgcgtacaag caggcccggc
      421 aggctgtcct cacagccaac catctcctgg accgtagccg ctccctggcc aatgctcatc
      481 tggagaccgc ggcgcagctg gcgttggatg agaagacggc cgcggacgcg aactcgacgc
      541 ttgaagcgct tgaggaggaa ggcgcgaatg gaacgaacac gctgaaggcg cctttcaagg
      601 gcatcgtcat gagcgtcagc gtgagttctg gtgcggtcgt tgcgcagggc acttcactca
      661 tcgaactggc caactccaaa ggactcgttt tcaagacggg ggtcgtgcct gcgcaggcgg
      721 cccggatcaa gaacggtgat gcagtcacca tgaccccgtt cagcggcagc ggaaccctgc
      781 acgggcgggt ttcgctgcgc ggatctgcgg tgaacctgga gaatggactc gttcccgttg
      841 atgtcaccat ctctgacggc aacccattga ccggggagat ggccagggcg gtgatcaaca
      901 cgggcgaaca acaggggtac gtcgttccgc atgacgcgat tttgctcaac gacagcggcc
      961 agacctatgt cgtgcagtcg gtgaatttgg tggccaagaa agtgccggtt accgtgatgg
     1021 gttcagcggg cgacacggat gtggtgaacg gcaagcttgt tgccggcgca ccgattgtcg
     1081 gcgcgggaag ctatcagctc gatgacggcg acaagatgcg gctgaccaat tcgactacca
     1141 gcgcttcggg cgggaaggct ggccaatgaa ctttatcgac tgggttgagc gtcacgcacg
     1201 ttccctcatt ttcattgcct tcgcgctgac gctggcgggt atttttgcgg cactcactct
     1261 tccgatccag ctctttccga ccgtcagttt tccccgcgtc cggattgaag tcgacagcgg
     1321 tgacatgccg gcgaaacaga tgctgatcga tgtcacggag cctttggaga aggtctcgcg
     1381 cgccgtcccg ggcgccgtca acgtgacgtc gacgaccact cgtggctccg cagaaatttt
     1441 tgtcgatttc ccgtggggat cggacatgca tcgcgccgtc ctgaacgtcg atgcggcttt
     1501 cgcgcagaaa ctgccggatc tgccccaggg cacgggctac gatgtgattc agatgagtcc
     1561 caacgcgatc atgccgttcg tgtcttatgc gttgatttcg gacaagatct ccccggcaga
     1621 cctcaagact ctcgccaagt atcagatagc gccgttgctg acggggattc ccggcatccg
     1681 gcaagtttct actctgggtg gtgaaaccag agaggtacag gtttacgtca gccctgagaa
     1741 gcttcagacc tatggtctta ccctgaccaa tctatcggac gccatctcgg gcacgaatac
     1801 gctggaggcg gtcggtcgta tccaggacaa cggcttgctc tatctgacca tcgagaacaa
     1861 ccagttcagt tccatcgagg cggttcgtga cgtaacgctg catacgtcga acggtggcgt
     1921 tgttcgcctg ggtgatattg cgaaagtgga gatggggtcc gtgccgcagt ggctgttggt
     1981 ggacgacaac ggacagccgt ccgtcaatat caacgttttt cagcaaaaca ccgcagacac
     2041 gctcacgctg gaaaaggagg ttcaagcaaa gctggacgcc tttatgaaga cccagccgaa
     2101 gtccatccag attcacaagt ggtacgacca ggccgaactc gtcaggtcgt cgataggggc
     2161 ggtagaggag gcaatcgtta tcggtcttat cctggctgcc tgcgtgattc tcggattctt
     2221 gcgcaactgg cgggcggcat ttgtcgcgat gatcacggta ccgatgtcag tgatgaccac
     2281 ttgcgtgctg ctgtcggctc tcgggttgag tttcaacatc atgaccctgg gcggaattgc
     2341 agctgcgatc ggactgttga ttgatgatgc catcgtcatg gtcgagcaga tcgcccgtcg
     2401 cgcgggagtt cccgggctgg aacaccctca gcgcacggtg ctttcttccg cgagggagtt
     2461 cctgtccccg ctgacggggt ccagcagtgc caccatcatc atgttcatcc cgctggcgtt
     2521 cctctcgggc gtaacgggtg catttttcaa gtccctgtcg ttgacgatgg cggcgtcgct
     2581 gatcatttct tacgggttca ctgccctctt ggtgccgtta atggcgcgtg gaatcatcga
     2641 cttcaaaaag tggcatgatc cttcgcacgg ccgcgagacg tggtcccgac gcactcatgg
     2701 acgcgtcttg acgcggttgt ttgcggcgcc ctggttaact gcaatcatcc tcgttgttct
     2761 tgccggtgtt ggctatcttg gctataccca cgtcggtacc ggcttcctgc cacggatgga
     2821 tgaaggtggc ttcgtgctcg actatcagac caagcccggt acgtcgctca tcgagaccaa
     2881 ccgcgagctc gcacaggtgg aagcgatcct ccaaaagaat tcatatgttg atacctacac
     2941 tcgccggacc ggggccggct tgggcggtga tctcaacctt ccttaccagg gcgatttttt
     3001 tgttcacctg gttcctccat ccaagcggcc accgatctgg caggtgatga attccctttc
     3061 ggcgcagatc aatagtcagg tcccggggat tgactacgat acgcaccagc tgctgtctga
     3121 catgattggc gatatggtcg gacgtcgtca gccggtggtt gtcaatctaa gcgccaagga
     3181 tccagacgtc cttcccggtg tggctgacaa ggttgcggat gcgcttaaga aagtcgacgg
     3241 gattgaaccg tcatcggtgg accccgggac tctgccgacc ggcgatgccc tggaaatcac
     3301 cgtggatcag gcgcaggccg cgatgaacca tctcacggcg tccgatgtca aggaccaaat
     3361 ctaccgttat ctacacggct ctgtcattac caaatacatg gggcgcgtac aggatgttgg
     3421 cgtgaggctt tgggtgcagc cacagaacac cggggatcgc gagaacacca gactctatcg
     3481 cgacgatttg tacaagctcc tgattcaggc gccaaacgga catacgcttc cgctcggagc
     3541 catagccaaa atcaaattcg tcagcgggca gcccgcgctc ttccgtgcca atctggcgca
     3601 aatcgtttcg ttgacggccc aaacaaacgg caagcgtgac ctcgggtcga ccattgccgc
     3661 ggtgaagcaa atcctggaca aacccggcac agtccctagc ggtgtctatt acacgttggg
     3721 cggcgcctac aagcagcagc aggaagcagc gcatggaatg gtcatcacgt tcagcgccgt
     3781 ggtgctggct gaaattatcc tgctgctctt tctgtacgaa cgcctctcgc ttccgttgat
     3841 catccttttc acgtcactgg tttccacggg agcggttttc acgggcctct ggctcactgg
     3901 caccgaactc aatatcaccg ccatgatggg catggtgatg atcctcggca tcgcgacaga
     3961 aatggccatc ttccttgtat ccgaatacca gatgctcgag aaggaaatgc cgccacgcga
     4021 ggcgatctat caggcagcgc tgaatcgatt ccgtccgatc ctgatgagta cgctggccat
     4081 gattcttgcc ttg
//
LOCUS       DATMTI010000868         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_840132, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000868 DATMTI010000000
VERSION     DATMTI010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840132"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /locus_tag="VN039_13745"
     CDS             <1..1815
                     /locus_tag="VN039_13745"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HWV47062.1"
                     /translation="KITVIVVGTGLAGASAASTLGQLGYQVECFCFQDSPRRAHSIAA
                     QGGINAAKNYQDDGDSVGRLFYDTIKGGDFRSREANVYRLAQVSTQIIDQCVALGIPF
                     AREYGGQLANRSFGGVQVSRTFYCRGQTGQQLLLGAYSALCWQVERGQVTMRPRTEML
                     DLVMVDGQARGIVTRDLVTGRVSIHTAHVVVLATGGYSNVYYLSTNASGSNVTAIYRA
                     WKQGAAFANPCFTQIHPTAIPPGDAHQAKLTLMSESLRNDGRLWVPTMRTDHRCPGDI
                     PAGERDYFLERRYPRFGNLAPRDLASRAVKSVCDEGRGVGPGGQGVYLDFADVIDRQG
                     LDVVRERYGNLFDMYQRITGEDAYDAPMRIYPAPHYTMGGLWVDYNLMSTIPGLFVIG
                     EANFADHGANRLGASSLMQGLADGYFILPYTIGHYLATAKLTSVGEDHQESRAALQRV
                     TSRVNRLLNSKGCRTAASFHREVGQLLWNHCGMSRNEEGLKSALTAIPALREEFWRDV
                     MVPGSGEAFNQELEYAGRVADYLEFAELLCHDALHREESCGAHFREEYQTDDGEPQRD
                     DNRFAHVAAWEYREGTSPVLHKEFLAFEFVQPTTRSYQ"
     gene            1831..2619
                     /locus_tag="VN039_13750"
     CDS             1831..2619
                     /locus_tag="VN039_13750"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HWV47063.1"
                     /translation="MKFTLNIWRQKGPDERGRFVTYEAHDVSSGMSFLEMLDSVNQRL
                     IAKGEEPVAFEQDCREGICGSCSLVINGIPHGPDRGITTCQLYMRRFPDGAIITAEPW
                     RARAFPIIKDLVVDRRALDRIMQAGGYISVHTGGAVDGNVLLINKDQAETAMDAASCI
                     GCGACVAACKNASAMLFVAAKVSHLALLPQGKPERTNRVNAMLEAMDREGFGSCGNQN
                     ECEAVCPKRISVRFIATLNREYLRAGVVESGLAAEPESPHAESA"
     gene            2876..>3256
                     /locus_tag="VN039_13755"
     CDS             2876..>3256
                     /locus_tag="VN039_13755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV47064.1"
                     /translation="MRPQSPRELIAALIWIVPYRCGHCCHRFLARRLSVAGPPPPMER
                     REHLRIPVRLCLSFSGGKVRGEGIVMDLSLGGCIIKSETQVHVDDIFYLEIVVAEDEP
                     PVEVAAMVRSVSARGIAFKFLRKAQ"
BASE COUNT          683 a          871 c         1001 g          701 t
ORIGIN      
        1 aaaatcacgg tgatcgtcgt cggcaccggc ctggcgggag ccagcgcggc ttcgaccttg
       61 ggacagctcg gctatcaggt ggagtgcttt tgctttcagg atagtcctcg ccgtgcccac
      121 agcattgcgg cacagggagg aatcaatgcc gccaagaatt accaagacga cggagacagt
      181 gtgggccgat tgttctatga cacgatcaaa ggcggagatt tccgctctcg cgaagccaat
      241 gtgtaccgac tcgcccaggt gagtacgcaa atcattgatc aatgtgtggc gctcggcatc
      301 ccgtttgcca gggagtacgg cgggcagttg gcgaatcgat ccttcggggg tgtccaggtc
      361 tcgcggacgt tctactgtcg tggacagacc ggacagcaac ttcttttagg tgcctattcg
      421 gcgctctgct ggcaggtaga acgaggacag gtcaccatgc gcccgcgcac cgagatgctc
      481 gatcttgtca tggtcgacgg gcaggctcga ggaatcgtga cgcgcgatct cgtcacggga
      541 cgagtgagta tccatacggc tcatgtcgtg gttcttgcga ccggcggcta cagcaatgtg
      601 tactatctgt cgaccaacgc gagcggcagc aacgtgacgg ccatctaccg tgcatggaag
      661 cagggcgccg cgttcgcgaa tccatgcttt acgcagatcc accccacagc gattccaccg
      721 ggggacgccc atcaagccaa gttgaccctg atgtccgaat cgcttcgcaa tgacgggcga
      781 ctgtgggtcc cgacgatgcg gacggatcac cggtgtccgg gcgacattcc cgctggagaa
      841 cgcgactatt ttctggaacg acgataccca cgtttcggca atctcgcccc acgagatctt
      901 gcgtcccgtg ccgtgaaatc cgtgtgcgat gaaggccgtg gcgtcggtcc gggtggtcaa
      961 ggcgtatatc tggatttcgc cgatgtcatc gaccggcagg ggcttgatgt ggtccgcgag
     1021 cgctacggca acctcttcga catgtatcag cggatcacag gggaggatgc ctacgacgca
     1081 cccatgcgca tttacccggc gccgcactat acgatgggag ggctctgggt ggattacaat
     1141 ctcatgagta ccatcccggg actctttgtg atcggcgaag cgaactttgc ggaccacggt
     1201 gccaatcggc tcggagccag ctcgctcatg caaggacttg ccgatggcta ttttatcctg
     1261 ccgtatacga tcggtcatta tttggcgacg gcgaagctga cttctgtcgg tgaagatcat
     1321 caggaatctc gggcggcgct tcagcgcgtg acgagcaggg tcaaccgtct cctgaattca
     1381 aaggggtgtc gaacggctgc ctcatttcac cgagaagtcg gtcagctgtt atggaatcac
     1441 tgtggcatgt cccgcaatga ggaagggctc aaatcggcgc tgacggcgat cccggcgcta
     1501 cgggaagaat tttggcggga tgtgatggtt ccgggatcag gagaggcctt caaccaagaa
     1561 ttggagtatg cggggcgagt ggccgactat ctcgaatttg ccgaactgct ttgtcacgat
     1621 gcattgcatc gggaggagtc atgtggggcg cacttccgag aagagtatca gacggatgat
     1681 ggcgagccgc agcgtgacga caatcgcttc gcgcacgtgg ctgcatggga gtaccgagaa
     1741 ggtacatcgc ccgtcctgca caaggagttc ctggccttcg agtttgtaca gccgacaacg
     1801 agaagctatc agtaatcggc aatcatcctc atgaaattca cgttgaacat ttggcggcag
     1861 aagggtccgg acgagagagg caggtttgtg acatatgagg ctcacgacgt gagctccggc
     1921 atgtcgttct tggaaatgct cgacagtgta aatcagagat tgatcgccaa aggagaagag
     1981 ccggtggcgt tcgagcagga ttgtcgtgaa gggatttgtg gaagttgctc gctggtgatc
     2041 aatggaatcc cgcatggccc ggaccgcggt atcaccacct gtcagctgta catgcggcgg
     2101 tttcccgacg gcgccatcat cacggctgag ccatggcgcg cacgggcctt tcccatcatc
     2161 aaagatctgg tggtcgatcg tcgagcattg gatcgaatca tgcaggccgg ggggtatatc
     2221 tccgtccata ccggtggagc ggtggacggg aacgttctgt taatcaataa agaccaggcc
     2281 gaaacggcga tggatgccgc gtcctgtatc ggctgtgggg cctgcgtagc ggcatgtaag
     2341 aatgcctcgg ccatgctgtt tgtggcggcc aaggtgtctc acttggcgct cttgccgcaa
     2401 gggaaacccg agcgtaccaa tcgggtgaac gcgatgctgg aagctatgga tcgagaagga
     2461 ttcggatctt gcggcaatca aaatgagtgt gaagccgtct gtccgaaacg catcagcgtc
     2521 cgtttcatcg ccactctcaa tcgcgaatat ctccgagccg gcgtcgtcga atccggtctt
     2581 gccgctgagc cggaatctcc ccatgcagag tccgcgtagc ggacagaccg acgacacggt
     2641 tttctcccct ttcgattcgt aaccaaacgc gatgccgagc gtcagccgcc gaccttccca
     2701 tggtgtaaaa ggttgatgga ggctgatttg cgccagatgg caatcagctt gactgtgaag
     2761 gaaaggcctt tatagtgcga taaggatgca gcaaaagttc tccagcggcc ggagatgaga
     2821 tcgaatgaat cgtcacgtga tctgcccgcg atgtcgaaaa gatggagcgc ttcgtgtgcg
     2881 gccacaatcg ccgcgcgaac tgatcgcggc attgatttgg atcgttccgt atcgttgtgg
     2941 acactgttgc caccggtttc tcgcccgtcg cctcagcgtc gccggacctc cgcctcccat
     3001 ggagcgccgt gaacatcttc gtattccagt acggctttgt ctatcctttt caggtgggaa
     3061 agtcagaggc gagggaatcg tgatggacct ctctttgggt ggttgcatca tcaagagtga
     3121 aactcaagtg catgttgacg acatttttta cctggagatc gtcgtcgccg aggatgaacc
     3181 tccggtcgaa gtcgctgcca tggtgcggtc agtcagtgca cgcggcatcg cgttcaagtt
     3241 tctccgcaaa gcccaa
//
LOCUS       DATMTI010000869         1838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_414983, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000869 DATMTI010000000
VERSION     DATMTI010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414983"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VN039_13760"
     CDS             <1..346
                     /locus_tag="VN039_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47065.1"
                     /translation="VGFVHAPVNGMGKQMGGKTSSHRAAALLRASLVHGHTHKRGMYD
                     DAKLDPSGPVSVMECGCALPWGEVEDYVNGSPVGWWWGVVFLKVCDGAVIDADWVSMA
                     TLKERYGQKLAA"
     gene            complement(585..658)
                     /locus_tag="VN039_13765"
     tRNA            complement(585..658)
                     /locus_tag="VN039_13765"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(624..626),aa:Gly,seq:tcc)
     gene            complement(664..738)
                     /locus_tag="VN039_13770"
     tRNA            complement(664..738)
                     /locus_tag="VN039_13770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(704..706),aa:Thr,seq:tgt)
     gene            complement(1322..>1838)
                     /locus_tag="VN039_13775"
     CDS             complement(1322..>1838)
                     /locus_tag="VN039_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47066.1"
                     /translation="RRCHKCCSPCQIKTWISSKPRIIAMTEILMKRVGYSLRPWDEMA
                     EQDMLELSDSGPLMVKVTKKRSLPHNRFYFACLRSMEKAGATSTVSEMHNAAKIKTGL
                     VAICQLPNGEYVAFPDSTAFDKMDQTEFNAWFPKAVEFWKSSGLWQWLAPDLREKVSE
                     GITIHDGREAA"
BASE COUNT          386 a          513 c          502 g          437 t
ORIGIN      
        1 cgtagggttc gtccacgcgc ccgtcaatgg aatgggtaag cagatgggcg gcaagacatc
       61 ttcgcaccgc gctgcggccc ttctgcgagc ctctttggtg catggtcaca cgcacaaacg
      121 cggaatgtac gacgacgcca agctagaccc gtctggccct gtgagtgtga tggagtgcgg
      181 ctgcgctctg ccatggggcg aggtcgagga ctatgtcaac ggatcgcccg tcggttggtg
      241 gtggggagtc gtcttcctga aggtctgtga cggcgctgta atagacgctg attgggtcag
      301 catggcgacg ttgaaagaac gttacggcca gaagctcgcc gcatagcata aagcccctgg
      361 cgttaaccgg gggctgaaca tgcctgccta caggcttacg gatagtaacc agccggggtg
      421 cgctttctgc gtggccggtc ttgttgggtt ttgaggtggt gagttttgca cttgccgatc
      481 cgggagtcga acccgtctgg tcagggactt gcacccctcc gcactcaccg ggctactacc
      541 gttcaccctt ttcctcaaac ttgttaccgc cgaagcggtg aaactggagc gggaaccggg
      601 actcgaaccc ggatcattag cttggaaggc taaggcacat cccttatacc actcccgcga
      661 atttggtgcc gcaccacgga cttgaaccgc gaaccaaccg cttacaaagc ggacgctctg
      721 ccattgagct agagcggcga aacttaggtg agcctttttg tgacatgctc aggtctgcta
      781 atggggccga agcgtaacgg cagattttct tcccataagg cagtcgaagg gcgaaccttc
      841 aagtcaccag tccctatttg tgtgatccga ggccgttaca ccagaccgaa ccaccatcat
      901 gacttgcctc accttacggc aaaggcccct catgtatttc gtgtgccatt gctggcgaaa
      961 cttgttgccg tctttccggc tgtcagaccg tctcggtcag cggaaccaat accctaccca
     1021 aaaacaccca taaaggcaag cgctcattct tcctactttg gaggtgtggg gattttggtg
     1081 aaccatttcg gcggcgtcac catcacccca tctcctttag agcggatcgg cctgtgtcgg
     1141 tgatgtaata gtttacgacc tttccgtcgc ggcgaccgcc agacagaagc ttttggcgac
     1201 gaagcttttc acacatccct cttttcctat cgtcgcggta tctaagcgac acttgaaaga
     1261 gtccttggtg ccgccttgac gggattggta caaaaactag cgtcaacgta aatcgtcgta
     1321 atcacgctgc ctctctccca tcatgtatgg tgatgccttc actgaccttt tcgcgcagat
     1381 ctggcgcaag ccattgccac aggccggacg atttccagaa ctcaaccgct ttgggaaacc
     1441 atgcgttgaa ctcggtctgg tccatcttgt cgaacgcggt ggagtctgga aaagcgacat
     1501 actcaccatt cggaagttgg catatcgcaa caaggccagt cttgatcttg gcggcattgt
     1561 gcatttcgct tacggttgag gttgcgccag ctttttccat cgaacgcagg caggcgaaat
     1621 aaaaccggtt atgcggcagg ctgcgctttt tcgtcacctt gaccatgagc gggccagagt
     1681 cggacagttc gagcatgtcc tgctccgcca tttcatccca aggccgcaga gaatagccga
     1741 cgcgcttcat caggatttcg gtcatcgcaa tgatccgtgg ttttgatgaa atccatgttt
     1801 taatctggca tggcgaacag catttatggc atcgtctt
//
LOCUS       DATMTI010000870         1704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_840198, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000870 DATMTI010000000
VERSION     DATMTI010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840198"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1118
                     /locus_tag="VN039_13780"
     CDS             315..1118
                     /locus_tag="VN039_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWV47067.1"
                     /translation="MQRSLLAAAMVGGLCSVIGVFVVLRGLAFVGAGTSHAAFAGVAL
                     GYLMGWPPLLLAIVFGLATVWITGWVEERGRMKLDVSIGILYTTTMALGILFIGLMKT
                     YNAEVYGYLFGSVLSVTPEELQIIGALSVLVLGLLLLFAKELYFIAFDQEMAEASGVP
                     ARQIFFLLLTLVALTVVVSLKTVGAILVFAMILIPASTAYQLTHSLAALTLYSAAIGI
                     LTAVAGVLISALWDVPSGPAIVLLATTMFFISVFFSPKRMRRPRFAHSH"
     gene            1273..>1704
                     /locus_tag="VN039_13785"
     CDS             1273..>1704
                     /locus_tag="VN039_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47068.1"
                     /translation="MHRQAFRAGVIGFVFTVGVALFPLSNGALAEEFGGTEPGKWILG
                     FRVGFSPLTQQLSEGTSTSVGPLVNFQGLYSVNTWLLVGMMLDWERHGVSLERPDIDL
                     GHQDTVSVLPTVELRPVKLGRIIPYVNMSFGVNINSFGEDTT"
BASE COUNT          340 a          448 c          455 g          461 t
ORIGIN      
        1 gctatagatg tgtgttagtg ctaacttacc atcaatcaac ccatctgagt tgattggtga
       61 catcaagtag agtcgaaatg cctgagcttg agcaagtttg ggctaagtct tggacgccat
      121 ctcagcggcg ataatagtac cacgaagcac ggttctacac tcctcgaata ttcttcttgc
      181 catccgaagt gccttcctgt atccccattg tgcttttggc ttatgcttct atgccggtag
      241 gtcacatagc ctttcattac gagtaactgc ccgataacat cagtctcatg ttcgacctcc
      301 tcgcctacga tttcatgcaa cgctctcttc ttgccgcagc aatggtggga gggctctgtt
      361 cggtcatcgg ggtgttcgtg gtactgcggg ggctggcgtt cgtgggagcc gggacgtcgc
      421 atgccgcatt cgcgggcgtg gcgctcggct acttgatggg atggccgccc ttgttgctgg
      481 ccatcgtgtt cggccttgcg acggtctgga ttaccggttg ggtcgaagaa cgtggccgaa
      541 tgaagctgga tgtctcgatc ggcattctct ataccacgac gatggcactg ggtattctct
      601 ttatcggact gatgaaaact tacaatgccg aggtgtacgg atacttattc ggcagtgttc
      661 tatcggtcac tccggaggaa ttacagatca tcggagcgtt gagtgtgctc gtgttgggtc
      721 tcctgttgct gtttgccaaa gagctctatt tcatcgcctt cgaccaagag atggctgagg
      781 cgtccggtgt tccggcccgg caaatatttt tcctcctctt gacattggtc gccttgacgg
      841 tggtggtttc actaaaaact gtcggcgcca tcttagtctt tgccatgatc ctgattcctg
      901 cctcgacggc ctaccaactc actcatagcc tcgcagcact gaccctctat tcggcggcaa
      961 tcggcattct gaccgccgtc gccggtgtcc tgatctccgc cctatgggat gtgccttccg
     1021 ggccggctat tgtcttactg gccaccacga tgtttttcat ttcggttttc ttctcgccga
     1081 agcgtatgcg gcgcccgcgg ttcgcccatt cccattaggt accttgctcc gttcttacga
     1141 taatgctatc tgtatggcat caccacctcg atcatgaggc tatcttcttc ttaatgtaag
     1201 aagattgcga gaaaggccga ttatttgatt gatctctcaa tagagaagga caagcacgag
     1261 aggagggatt tcatgcatag gcaagcgttc cgagcaggtg tcatcgggtt cgtgttcacc
     1321 gtcggcgtcg ctctattccc cctttcaaac ggcgcgctcg cagaagagtt cgggggaaca
     1381 gaaccaggca aatggatact gggctttcga gtgggatttt cgccactaac ccaacaactc
     1441 tccgaaggca cctcaacctc ggtcggaccg ctcgtcaatt ttcaaggttt gtacagtgtg
     1501 aacacttggc tcttggtcgg aatgatgctt gactgggaac gccacggagt cagcctggag
     1561 cgacctgaca tagacctcgg acatcaagat acggtgtcgg tgctgccgac agtggaactg
     1621 cgcccggtga aattaggtcg gatcatcccg tatgtgaaca tgagcttcgg cgtaaacatc
     1681 aatagctttg gggaagatac gact
//
LOCUS       DATMTI010000871         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_32525, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000871 DATMTI010000000
VERSION     DATMTI010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32525"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VN039_13790"
     CDS             complement(<1..453)
                     /locus_tag="VN039_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47069.1"
                     /translation="MIKFPANQPFSRALSASSSRTFLTLCALALTLSTLSGCGESNVK
                     IPVVPSRTELPSLAPSKVILTPAQEQDIQIQLATAHMEPIGDSLQTTGKVQAISNLMG
                     HTYCPVPGQAASVLVSIGQNIHQGQPLAWIRSDQIGQLETDFLQNYLQN"
     gene            complement(446..>2022)
                     /locus_tag="VN039_13795"
     CDS             complement(446..>2022)
                     /locus_tag="VN039_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV47070.1"
                     /translation="AMSRPRLALGLMVIPVILSVIVFLGIGTEFLPSLDEGNIWLRVT
                     VLPSSVALEQSVGVANEVREKLMQYPEVKNVVSQVGAPDDGTDPNNPSNIEFLVDLKL
                     AKDWRPKFHEDKEALIASMSDDLSVIPGILTNFSQYIQDNVDEAIAGAKGQVVVKVYG
                     NELNTLQKLGVQIGKLFNQVKGMEDVAADKLVGQPQYQIEVNRPEADRYGINVEDITK
                     LIETAVGGKRATQIIEGEKRFNVMVRFKKAFRSSQEALQNVLVETPDHAQIPLSEVAN
                     LKMKTGANIIYRSDNSRVIVIKGNVRDRDLGSVVSEAKARIEQNVHLPVGYTMKWSGQ
                     FENQQRANSRLSVAVPLTILAIFLLLFLTFGNLPEALMAMTPIPLTMIGGIAGMWLTH
                     TYFSVSAGVGFIAAMGVSVQNGVVILSYIKNLTGRGMSPYLAAVNGARAKIRPVIMAG
                     TVAILGLIPAAISNGIGSQSQKPFAIVIIGGLFTATTLTIFAIPVIYYLINRHKAESL
                     SAAESSHHQPGAHSHD"
BASE COUNT          421 a          543 c          597 g          461 t
ORIGIN      
        1 gttttgcagg tagttctgca aaaaatccgt ttccagctgg ccaatctgat ccgagcggat
       61 ccacgccagc ggttgtccct ggtggatgtt ttgcccgatg gagaccagga cgctggcggc
      121 ttgtccaggt accgggcagt aggtgtgccc catcaggttg ctgatggcct gcaccttgcc
      181 cgtggtctgc aaggaatccc caatgggctc catatgtgcc gtggctagct gaatctgaat
      241 gtcctgctcc tgggctggcg tcaaaatgac cttggaaggc gccaggctgg gcagttcggt
      301 gcgagaggga actaccggaa ttttgacgtt gctctcgcca cagccggaca aggtggaaag
      361 agtgagcgcc agcgcgcaca aagtgagaaa cgtgcgagaa gacgacgcgc ttagtgcgcg
      421 agaaaaaggc tgattggctg ggaatttaat catgggagtg agctccaggc tgatgatggc
      481 ttgattctgc tgcggagaga gattcggcct tgtgccggtt tataaggtag taaataaccg
      541 ggatggcgaa aatggtcagg gtggttgcgg tgaataatcc gccgatgatg acgatggcaa
      601 atggtttctg gctttgtgag ccgatgccgt tcgatatcgc tgcgggaatc agtcccagga
      661 tggcgacggt gcctgccatg atcacgggcc tgattttggc ccgggcaccg ttgactgcgg
      721 ctaggtatgg cgacatgcct cgtccggtca gattcttgat ataggagaga atgaccacgc
      781 cgttctgaac ggaaacaccc atggcggcaa tgaagccgac gcccgctgag acggagaaat
      841 aggtgtgcgt cagccacatt ccggcaatgc ccccaatcat ggttaggggg atgggcgtca
      901 tggccatcaa cgcctccggc aggttcccga aggtcaggaa cagcaacagg aagatggcca
      961 gaatcgtcaa tggcaccgcc acggagagac gactattggc acgttgctgg ttctcaaact
     1021 ggccgctcca tttcatcgtg taaccgacgg gcaggtggac gttttgctcg atccgggctt
     1081 tggcctcgct aaccacactg cccaggtcgc gatcgcgaac gttgcccttg atcacgatga
     1141 cgcgggagtt gtcggagcga tagatgatat tggccccggt tttcatcttc aggttggcca
     1201 cttccgatag gggaatctgg gcgtggtccg gggtttcaac cagcacgttt tgcaaggcct
     1261 cctgcgagga acgaaaggcc ttcttgaacc ggaccatcac attgaagcgc ttctccccct
     1321 caatgatctg ggttgcgcgc ttaccgccca cggccgtttc aatcagcttg gtaatatctt
     1381 ccacgttgat gccgtatcga tcagcctcgg gacgattgac ctcgatctgg tactgaggct
     1441 ggccaaccag tttatcggcg gccacgtctt ccatgccctt gacctgattg aacagcttgc
     1501 cgatttgtac gcccagtttt tgcaaggtgt ttagctcgtt tccatagact tttaccacca
     1561 cctgaccttt agcgcctgca atggcctcat ccacgttgtc ctggatgtac tgggagaagt
     1621 tggtcaggat gcccggaatg accgataggt catcggacat agaggcgatg agcgcttcct
     1681 tgtcttcgtg gaacttgggt ctccaatctt tggccagctt caggtcaacc aggaattcaa
     1741 tgttgctggg gttattggga tcggtgccgt catccggcgc acccacttgg gacaccacgt
     1801 tcttcacttc gggatactgc atcagcttct ctcgaacctc attggccaca cccacggatt
     1861 gctccaatgc gacactgctc ggtaagaccg taacgcgcag ccagatattc ccttcgtcca
     1921 ggctgggcag gaactcggtt ccgattccca gaaagacgat cacgctcagg atcacgggaa
     1981 tcaccatcag gcccagggcc agccgaggtc tggacatggc cc
//
LOCUS       DATMTI010000872         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_754078, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000872 DATMTI010000000
VERSION     DATMTI010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754078"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VN039_13800"
     CDS             <1..519
                     /locus_tag="VN039_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWV47071.1"
                     /translation="VDFHQDCAYLDRLINRLQEIRALEKDEKGELYWLRDQWRHQPRM
                     WEDQQVLVHGDAPPEHFMVGDGLQVMAFDLERAKHADRVFDTGRIAGELKHFFLRATG
                     KTDAAEPFIGHFLWEYACHFPDRDRAFQSITGRTPFYLGMTLLRIARNHWIDPDYRRR
                     LIHEATACLRAL"
     gene            520..1263
                     /locus_tag="VN039_13805"
     CDS             520..1263
                     /locus_tag="VN039_13805"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWV47072.1"
                     /translation="MHVKGIIFDINGTLSDIHTDEGHDEVYRVLSNLLSYQGIVLEPH
                     VVKDLYFQIMKEQRAAQGGRHPEFDVSGIFHTIITRHATDFTRGLPTQKLEQLPRLLA
                     ETYRAASRFRLQPYPGVVDTIRHLHQNYHLAIISDGQTAYAVPELNAVGLAGYFDPII
                     ISGDVGVRKPHERLFTAALTAMQMKPSEVLFVGNDMYRDVYGAKRVGMTTVFCKSNQG
                     LMEKEGVTPDYIIYQFPELLNAIRFFEAQ"
BASE COUNT          371 a          468 c          461 g          335 t
ORIGIN      
        1 gtggatttcc accaagactg cgcgtatctg gatcgcctca ttaacagact gcaggaaatc
       61 agggcactcg aaaaagacga gaaaggcgaa ttatattggc tgcgagacca gtggcgtcat
      121 cagccgagga tgtgggaaga ccagcaggtg ctcgtgcatg gggacgcgcc cccggaacac
      181 ttcatggtcg gggacggatt acaagtcatg gcgttcgatc tggagcgagc caagcatgcg
      241 gatcgcgtgt tcgataccgg ccgcatcgcc ggagaattaa agcatttctt tctgcgggct
      301 accggcaaga cggacgctgc ggagcccttc atcggccact ttctttggga gtatgcctgc
      361 cacttccccg accgcgatcg cgcctttcaa tcgatcacgg ggaggacccc gttctacctc
      421 ggcatgacgt tattacgcat cgcacgaaac cattggatcg acccggatta tcgtcgtcgc
      481 ctgatccacg aggcaacagc ttgtttgagg gccctgtaaa tgcatgtcaa agggattatt
      541 ttcgatatca atggcacgct ttcggatatt cacaccgacg aggggcacga cgaggtctat
      601 cgggtcctga gcaacctgct ctcctatcag ggcattgtgc tcgaaccgca cgtcgtgaag
      661 gatctctatt tccagatcat gaaggagcaa cgcgcggcac agggcggccg gcaccctgaa
      721 tttgatgtga gcggcatctt ccacacgatc atcacccggc acgcgacgga cttcacacgc
      781 ggactgccga cgcagaaact ggagcaactc ccgagactgc tggccgagac gtaccgtgcc
      841 gcctcacgct ttcggttgca gccctatccc ggcgtcgtgg ataccatccg gcacctgcat
      901 caaaactatc atctggccat catttccgat ggtcagaccg cctatgccgt tcccgagctg
      961 aacgccgtcg ggctcgccgg gtatttcgac ccgatcatca tctcgggaga cgtcggcgtt
     1021 cgtaagcccc acgaacgcct gttcacggcc gcattaactg ccatgcagat gaagccgtca
     1081 gaggtgctgt ttgtgggcaa tgacatgtat cgcgatgtgt atggggcaaa gagagtcggg
     1141 atgaccacgg tcttttgcaa gtcaaaccaa ggcctgatgg aaaaagaagg cgtcacaccc
     1201 gactatatta tttaccagtt ccctgagttg ctcaatgcca tccgcttctt cgaggcccag
     1261 tgagtcatcg gatgaaccag atggcgtgtt cgcgtgtacc ctgggccaag gctgaggcgt
     1321 tcgcctaacg gcatcgatgc cacggcgcag gtcaaggccc cgccatcctg agactatcgt
     1381 catcagcctg tcggtgaaca ttgacgacga aaaccaggag gcgatggtga aggccggcgc
     1441 gaccctattg atgtccaaag aagcggctat cgatcagcta catgaaacaa tccacgccgc
     1501 tgcacaattg cggcagatgt gctcacgaat aatgtgggat aggatcagac tcggtagcgc
     1561 aggaagagat ggccgctctt ggcccgtttc acggacagca acgttcccca gatcgcttgc
     1621 ttaggaggta acccc
//
LOCUS       DATMTI010000873         1868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_160191, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000873 DATMTI010000000
VERSION     DATMTI010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160191"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1832
                     /locus_tag="VN039_13810"
     CDS             207..1832
                     /locus_tag="VN039_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HWV47073.1"
                     /translation="MRTIKRVLTFIGPWCAAVLACSIGLSAYGCKDSASVSDEPQVPL
                     SSLAVAPGTLQPAFSSNISNYTVDAPTAATSVTVTAAPTDSTTTMTINGVSTAAGQGR
                     SVLLGPPGSTATIHIALSSQTGGEGAYTVTVTRLLSSDNNLSALSVTSGSLDPSFASD
                     KLTYTVDVATDVTSVNVSATKSDPNAVIAGSVTAAAGVPTGQATIALDGPGTSKILSL
                     TVTAPNGSSKTYGVTVHRAASSNNNLSALSVTPGDLAPRFDPRTLTYRVNVETKVTSV
                     TVSATKSDPNAVISGDVPNEGQATIQLGGPGTTTQVEITVTAQSGSSKTYRIDVERAA
                     PSSDNNLLALSVTPGTLAPSFAANTLSYTMDVATDVTEVTVAATKSDPDAVMSGSLTA
                     GTGVATGQATLPLGGPGTTTQVSITMTAPNGSSKTYTIGVNRAAPSTDNNLSNLTVSA
                     GSLVPAFAPGTPAYSVDVSAADETITVRATKSDPNAVMSASGSVIAAAGIPAGQVTVP
                     LGLGTSTPVTISITAQDGISTKAYTITVNRPSR"
BASE COUNT          431 a          593 c          486 g          358 t
ORIGIN      
        1 gccgccggct gtgcccgttt tgcactatac acacatcggt gcaccacgcc tcgagtcgaa
       61 gtcttgaccc caccagtaga attggctata cctttaatcc ctgcttcggc catatagaga
      121 ccggaccctc tcgtagcttc catacctcgc gtttgggttc atcagaccgg atgcgcccat
      181 gacgtcatac gccaagggac agtagtgtgc gcacaataaa acgcgttctg acttttatcg
      241 gaccatggtg cgctgccgtc ctcgcctgct ccattggctt gagtgcctat ggctgcaaag
      301 attcggcgtc ggtttcggac gagcctcagg taccgctctc cagtttagcg gtcgccccag
      361 gcaccctcca gccggcgttt tccagcaata tttcgaacta tacggtcgat gcgcctacgg
      421 ccgctaccag cgtcaccgtg acggccgcgc ccacagacag cacgaccacg atgacgatca
      481 atggcgtttc aaccgctgca gggcagggac ggtctgtact cctaggcccg ccgggatcca
      541 ctgccaccat ccacatcgct ttgtcatcac agaccggagg tgagggcgcc tataccgtca
      601 ccgtcacgag actcttatcg agcgacaaca atctgtcggc cttgagcgtc acatcgggat
      661 ccttagaccc ttcctttgct tcagacaagt tgacctatac ggtggatgtt gcgaccgatg
      721 tcaccagtgt gaacgtctcc gcgaccaagt ccgatccgaa tgctgtgata gccggttctg
      781 taaccgccgc cgcgggcgtt cccacgggac aagcgactat cgcgctggac ggaccaggaa
      841 caagtaaaat tttatcactc accgtgaccg ccccgaatgg aagcagcaag acctacggtg
      901 tcaccgtcca ccgagcagcg tcgagcaata acaatctgtc agccttgagc gtgacacccg
      961 gcgacttagc tccgagattt gacccgagaa ccctgaccta ccgagtgaac gtagagacca
     1021 aggtcaccag cgtcactgtc tccgcaacca agtccgatcc gaatgcggtc atatccggtg
     1081 atgtgcccaa cgaaggacaa gcgaccattc aactcggcgg gcctgggacg accactcagg
     1141 tggaaatcac ggtgactgca cagagtggaa gctcaaaaac atatcgcatc gacgtcgaac
     1201 gagcagcccc ttcaagcgac aacaacctgt tggcattgag tgtaacaccg ggcaccctgg
     1261 ccccctcttt tgctgccaac acactgagct acacgatgga tgtggcgacc gatgtcactg
     1321 aagtgaccgt cgctgcaacc aaatccgatc cggatgccgt gatgtccggt tcgttaacgg
     1381 ccgggacagg agttgccacg ggacaagcaa cgctaccact cggcgggcca gggacaacca
     1441 cccaagtatc aatcacaatg actgccccga acggaagctc gaagacctac acgattggcg
     1501 tgaaccgggc ggccccttca accgacaaca atctctctaa cttgaccgtg agcgcaggtt
     1561 cattagttcc ggcttttgct ccgggaacgc cggcctacag cgtggacgtg tctgcggccg
     1621 acgaaaccat aaccgttcgt gcgaccaagt ctgatccgaa tgccgtgatg tccgcatccg
     1681 gttcggtgat cgccgcggca ggaatcccgg caggtcaagt caccgtccca ctggggctcg
     1741 gaaccagcac acctgtgacg atctccatca ccgcacagga tggaatcagc acaaaggcct
     1801 acaccattac cgtgaaccga ccatcccgtt aaattcaata cgaggagaac cggcgtggga
     1861 atagctca
//
LOCUS       DATMTI010000874         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_500137, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000874 DATMTI010000000
VERSION     DATMTI010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500137"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..340
                     /locus_tag="VN039_13815"
     CDS             155..340
                     /locus_tag="VN039_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47074.1"
                     /translation="MKVKKLKVGVRSLEESLQEFVHADLELLADLDLVQLQSGAHLRG
                     SATPTIPYERIQFEIAV"
     gene            complement(434..1915)
                     /locus_tag="VN039_13820"
     CDS             complement(434..1915)
                     /locus_tag="VN039_13820"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWV47075.1"
                     /translation="MRQVRGHELGRGGSLRRMGMAFGILTMLLTTVVVAYSADPTISD
                     PRTMSFKPVEFSPPEPERVVLDNGMVVYLLEDHELPLVTITATMRTGSWLDPTDKIGL
                     AGMTGAVMRTGGGGGLSAEQVDTELEQFAGEVSIGIGRQSGSASLDVLSKDVNRGLEI
                     FVGLIRTPTFDPSRVELAKLQAIEGIRRRQDNPGSVVGREFVKMLYGADHPSARESSV
                     DSIKRITRDDLVTFHRNTIHPNGMILGVTGDFKRDEMIASLRKMLGDWKKGTVPELRI
                     SDVPTEELSRSAVRFVGKDTSQTHLRVGHLSIKENDPDYVALAIANDILGGSSFRSRL
                     FNDVRSKRGLAYSVGSRLNTGMHDQGIWQMRAETKLISTQEVIERFVANIERMRVEPV
                     TDAELAEAKEAYVNSFVFSFSSPSAIVSRLVELEYDGLPKDFLQQLRAKVVKLTKDDV
                     LAAAKKHLHPGRLKIVAVGSGEALPKALATFGDVKEIKLKPEG"
     gene            complement(1912..>3083)
                     /locus_tag="VN039_13825"
     CDS             complement(1912..>3083)
                     /locus_tag="VN039_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWV47076.1"
                     /translation="AVVESLQNRLMELQTQAAQYVVGNEMALLYQRHGGVGLNASTGK
                     DVTRYMISLPSNRLPLWAAIESDRMANPVLREFYKERGVVMEERRLRNDDSPNGLLFE
                     TFTSAAFRAHSYGVPTIGWGSDILSLTPADTEAFFKAHYGPDQATIALVGDINPKETI
                     ALIEQTFGKIPAAPPPPPLVTVEPEQRGERRVEVEFDAEPAIVIGYHKPALGHPDDDV
                     FDVIDAVLSDGLTSRLHQTLVREKRLAASVGSDANHPGVRAPNLFIFTATPLAPHTTA
                     EVEAAVYEEVERLKREPVSSKELEKVLNNLDADLVRGLRSNSGLASQLALYQAVAGDW
                     RYILKSRDKVAAVTAADVQRVAKQYFTKSNRTVAVLVKKGTDKTVAAIPIDEVKP"
BASE COUNT          669 a          956 c          791 g          667 t
ORIGIN      
        1 agtaccatga ctctgtcact tgggtgaaac tccatcctcg ggtacctcac acgcttcaca
       61 gtaccgcgcc atgtggttgg tgatcaaggt aatgaagggt gatgcgatat gacgttcgtc
      121 gcgatatcac ggcagtcaag aaggagttgg gacaatgaaa gtcaagaaac tgaaggtagg
      181 agtgcgttca ttagaggaaa gcctgcaaga attcgtgcat gccgatctgg agctcctcgc
      241 cgacctcgat ctggtgcagc ttcaatcagg ggcgcatctg aggggctccg ccactccgac
      301 tattccttat gaacgaattc agttcgaaat cgcggtgtga acccacatga gaaatcgtct
      361 caggccatca ggcggctccg tcgtatcatg gcgacgccgg agctggccgc gcttcgccag
      421 ccacggaact tcttcaccct tccggtttca gtttgatttc tttcacatct ccaaacgtcg
      481 caagggcttt cggcaaggcc tcccctgacc cgacggcgac gatcttcaaa cgacccgggt
      541 gcaaatgttt ctttgccgcc gctaagacgt catccttggt cagcttcacc accttggcgc
      601 gcagctgttg gagaaagtcc ttcggcaatc catcgtactc taactcgacc aaccggctga
      661 cgatggccga agggctggaa aatgaaaaca cgaacgaatt gacgtacgct tccttcgcct
      721 ccgcaagctc ggcatcggtc acaggctcga cgcgcatccg ctcgatattc gccacaaacc
      781 gctcgatcac ttcctgggtg gaaatcaact tggtttccgc ccgcatctgc cagatacctt
      841 ggtcatgcat gcccgtattg agtcggctgc cgacggaata ggccagcccc cgtttcgacc
      901 gcacatcgtt gaacaggcgg ctacggaatg aactgccacc caaaatatcg ttcgcgatgg
      961 ccaatgcgac atagtcggga tcattctcct tgatcgaaag atgtcccact cgaagatggg
     1021 tctgcgaggt atctttcccg acgaacctga cggcagatct ggacaactcc tctgtcggca
     1081 catcggaaat tctcagctcc ggcacggttc cctttttcca atctccgagc atcttacgga
     1141 gagaggcgat catctcgtct ctcttgaagt caccggtcac accgaggatc ataccgttcg
     1201 ggtgaatcgt gttgcgatgg aacgtgacga gatcgtccct ggtgatgcgt ttgatcgagt
     1261 ccaccgagct ttctcgcgcg ctcggatgat cggccccata gagcatcttg acaaattctc
     1321 ggccgacgac ggagccggga ttatcctgac ggcggcggat cccctcgatg gcctgtaact
     1381 tggccagctc gacacgggag ggatcgaacg tcggagtccg aatgagaccg acgaagatct
     1441 ccaacccccg attcacatcc ttgctcagga catcgagaga cgccgacccg gattgtctgc
     1501 cgattccgat gctgacctcg cctgcaaatt gctccagttc cgtatcaacc tgctccgcgg
     1561 aaagaccgcc gccaccaccg gtccgcatca ccgccccggt catgcccgcc agtccgatct
     1621 tgtcggtcgg atcgagccag ctacctgttc gcatggtcgc cgtgatcgtg accaggggta
     1681 attcatggtc ttccaataaa tagacgacca tgccgttgtc cagaacgacc cgttccggct
     1741 ccggtggtga aaattctacc ggcttaaacg acatggtcct gggatcgctg atggtcggat
     1801 cggctgaata ggcgaccaca acggtcgtca gcagcatcgt cagtatgcca aaagccattc
     1861 ccatgcggcg aagactccca ccccgtccca attcgtgccc ccttacctgc ctcatggctt
     1921 cacctcgtca atgggaatcg ctgcaacggt tttatccgtg cctttcttca ccaagactgc
     1981 aacggtacga ttcgacttgg taaagtactg cttcgccacc cgctgtacat cggcagccgt
     2041 gaccgcagct actttgtcgc gcgacttcaa gatatagcgc caatcaccgg ccaccgcctg
     2101 atacaaagcg agttgagaag ccagaccgct gttcgatcgc agaccccgca ccaaatcggc
     2161 atccaaattg ttcagcacct tctccagctc cttggaagag accggttcac gcttcagccg
     2221 ctcgacctcc tcatagacgg cggcctctac ttccgctgtc gtatgagggg ccaacggcgt
     2281 cgctgtgaaa atgaataggt tcggtgcacg cacacccgga tgattcgcat ccgacccgac
     2341 tgaggcagcc agacgtttct cccgcaccaa cgtctgatgc aagcgagacg tgagtccgtc
     2401 gctcagcacc gcatcgatca cgtcaaatac atcgtcatcc ggatgcccca aggccggctt
     2461 gtggtacccg atgacgatag ccggttccgc atcaaattcc acctcaactc gtcgctcgcc
     2521 ccgttgttcc ggctcgaccg tcactaaagg cggcggcggc ggcgcggcag gaatcttccc
     2581 gaacgtctgt tcgatcaagg caatcgtttc cttcggattg atatcaccca ctagagcaat
     2641 cgtggcttga tccggaccgt agtgggcctt gaagaaagct tccgtgtcgg ccggtgttaa
     2701 tgacaggata tcggatcccc atccgatggt cggaacacca taactatggg cccggaaagc
     2761 agcggatgtg aacgtctcaa acaacagccc attggggcta tcgtcgtttc gcaagcgccg
     2821 ttcttccatc acgacaccgc gctctttata gaattcgcgc aacacagggt tggccattcg
     2881 atccgactcg atcgccgccc acaaaggcaa tcgattggac ggcaaactga tcatgtaccg
     2941 cgtcacgtct ttgcccgtcg atgcattgag tcccaccccg ccgtgccgct ggtacaacag
     3001 agccatctca ttcccgacga catactgagc ggcctgtgtc tgtagttcca tgagacgatt
     3061 ctggagtgat tcgaccactg ccc
//
LOCUS       DATMTI010000875         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_500164, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000875 DATMTI010000000
VERSION     DATMTI010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500164"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1126)
                     /locus_tag="VN039_13830"
     CDS             complement(260..1126)
                     /locus_tag="VN039_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV47077.1"
                     /translation="MTQQRDGTNTERIHTSEPRYRLAATGRQETEDERLSLLEDIFDP
                     LSRQRRAFVQPGWRCLEVGAGRGSMAVWLAKQVGANGRVVATDVDTIYLNRLDLPNLE
                     VRRHDILSDSLDALDPGSFDLVCARLLLFWLAGKQENAIRRMVECLRPGGWLIDEDGD
                     WGMVAPVDPSHSHYALYHRVWKNGEWWAARGYDPTFGRKLPMLFERCGLVNIRHKASA
                     TVVRGGSPWGRWWLKSMEAIRASEQTDGSLTEERDQEYGVLTAPLTDPSFWFLNALIH
                     ACWGQRGARRDE"
     gene            1369..>2385
                     /locus_tag="VN039_13835"
     CDS             1369..>2385
                     /locus_tag="VN039_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47078.1"
                     /translation="MKHPLLVSSLVVLLVGIASQAWCSDGIFLNTQIPYRYIGFSAPQ
                     TGDPISAQAERVRIILTSYVHHQSIVISRLRDPTSDAEPPRIDQLPATTTWLSGKLGT
                     EAEVAYNPTGVSQPMPSIMGSTQTDASARMFRIGLIGSEGSLRYGVAFRHAGQGFLLS
                     PDRASREIWSEWKTGWMTFRNAVGQTWNNVEGEATRSRLEQTYGRVGLFLSKPLWPEL
                     SVSYSRNSLNSVFEPIGMAPQRSSNHTIEGAVTLQRPGWDIGFASSYILASDLLRSST
                     NSNVRAQTISAILRPINQLTITPVFAYRQEFQSWSGVRIDSPTASFALSYQQSPQLLF
                     STVGN"
BASE COUNT          528 a          688 c          646 g          523 t
ORIGIN      
        1 agcggacgat gtcgagaaaa tgtacgctgc ttgggtcaag tcctgcctcc ttcaagtgac
       61 cctctggagc catccctacg taaaggccgg agatttttaa ttcggcgccg gacagatttc
      121 ggggacattc cgaccaaaac cgaggaggct ctcggaacgg ttcgggatgt ccctccttca
      181 gaggcttagg agaagatcca tcctggcatc ggacactgcc ggctcaccat ctcgccggcg
      241 cgcacagacg tggcgctcct tattcgtcgc gccgtgcgcc tcgttggccc caacaggcat
      301 ggatgagcgc gttgagaaac caaaaagatg ggtcggtcaa aggggcggta agcactccgt
      361 attcttgatc tcgttcctcg gtgaggctgc catcggtttg ttcagaggca cgtatcgctt
      421 ccatactttt caaccaccac cgtccccagg ggctgcctcc acggaccacc gttgcgctcg
      481 ctttgtggcg gatattcacc aagccgcagc gttcgaacaa catcggtaac tttcgtccaa
      541 acgtaggatc gtatccgcgc gccgcccacc actcgccgtt cttccagacg cgatgataga
      601 gggcgtagtg tgaatgagaa gggtctaccg gggcgaccat gccccagtca ccatcctcgt
      661 cgataagcca tccgcccggt cgcaagcatt ccaccatgcg acgaatggcg ttctcctgtt
      721 tacctgcaag ccaaaataac aggagccgtg cacagacaag atcgaaagaa ccgggatcca
      781 gcgcatccag tgaatcgctg aggatgtcgt gccgacggac ttcgagattc gggaggtcga
      841 gccgattgag gtagatggta tcgacatcgg tggccacgac tcgaccgttg gccccgactt
      901 gcttggcaag ccacacagcc atagacccac ggcctgcgcc gacctcgaga cagcgccaac
      961 ctggttgaac gaaggcacgc cgttgacgcg aaaggggatc gaagatgtct tcaagaaggc
     1021 tgagccgctc atcttcagtc tcctgacgtc ccgttgcagc aaggcgatac cgtggttcgg
     1081 atgtatggat gcgttcggtg ttcgttccat ctcgctgttg tgtcacgcct gttaccgtaa
     1141 tcggctaccc gttgacctgt caatcttgcc tgagctgact taatggcaaa ggcttggatc
     1201 cctttagaac cggcttgagc gccaagtacg gtgatttatc gacgcggcgt ccttcttttg
     1261 ctcatcctga tctgtgggat gatcaaatct atcaatatag tccaagactt aagatgataa
     1321 ttctgactgc tacacttcag aaggaccggt tcatggctgg aatcatacat gaagcatcct
     1381 ctattggtat cgagcctagt ggtgttgctt gtgggcatcg cctctcaggc ctggtgcagc
     1441 gacggtattt tcctgaacac acaaatcccg tatcgttaca ttggattctc ggctccgcag
     1501 actggtgacc ccatttctgc tcaggccgaa cgagtccgaa tcatcctcac atcatatgtc
     1561 catcaccagt ctattgtgat ctctcgtttg cgggatccga cttcggatgc tgagcctcca
     1621 cggatcgacc agctgccggc gacaacgaca tggctcagcg gaaagttagg gaccgaggcg
     1681 gaggtcgcat acaatccaac gggtgtgagt cagccgatgc cgtcgatcat gggaagtaca
     1741 caaaccgatg catcggcacg catgttcagg attgggctca tcggctcaga gggaagtctt
     1801 cgatatggag tggcatttcg ccatgcagga caagggttcc ttctctcccc tgaccgagcc
     1861 agccgcgaaa tttggagcga gtggaaaaca ggatggatga cgttccggaa tgccgtcggt
     1921 caaacgtgga acaacgtcga aggagaagcg acacggtccc gattggaaca gacctatggg
     1981 cgggttggac tgtttcttag caagccctta tggcccgaac tgagcgtctc ctattcgaga
     2041 aactccctga atagcgtgtt cgaaccgata ggaatggctc cgcagcgatc ctcgaaccac
     2101 acgattgagg gtgccgtgac tctccagcgc cccggatggg atatcggatt tgcgtcgagt
     2161 tacatcttgg cgagcgacct gctccgcagc agcaccaaca gtaacgtcag agcacaaacg
     2221 atttcggcga tccttcgccc gatcaaccag ctgaccatca caccggtttt cgcctatcgc
     2281 caagagtttc aatcctggtc cggcgtacgt atcgacagcc caaccgcctc ttttgctcta
     2341 agctaccagc aaagcccgca gctccttttc agcaccgtgg gcaac
//
LOCUS       DATMTI010000876         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_669165, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000876 DATMTI010000000
VERSION     DATMTI010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669165"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /gene="aroC"
                     /locus_tag="VN039_13840"
     CDS             complement(<1..774)
                     /gene="aroC"
                     /locus_tag="VN039_13840"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWV47079.1"
                     /translation="MACNTFGRIFTVTSFGESHGTAIGCVIDGCPPSLSLSTEDIQKD
                     LDRRKPGTSRHVTQRQESDRVEILSGLFEGQTTGAPIALLIRNEDQRSRDYGNLIDSF
                     GPGHADYTYWQKYGIRDHRGGGRASARETAVRVAAAAIARKWLSEKYGVVIRGYLSQL
                     GPHELAFKDWDAVGRNPFFSADPDVVPKLETFMDELRKDGDSVGARITTVAEHAPVGW
                     GAPVYGKLDADLAAAMMSINAVKAVEIGAGFASVAQRGSE"
     gene            complement(790..1875)
                     /gene="aspS"
                     /locus_tag="VN039_13845"
                     /pseudo
     CDS             complement(790..1875)
                     /gene="aspS"
                     /locus_tag="VN039_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
BASE COUNT          415 a          589 c          478 g          393 t
ORIGIN      
        1 ttctgaacca cgctgcgcga ccgacgcgaa gcctgcccca atctcgaccg ccttcaccgc
       61 attgatgctc atcatcgcgg cggccaaatc ggcatccaat ttcccataga ccggcgctcc
      121 ccacccaacc ggcgcatgct cggccacggt cgtgattctc gcgccgacag agtcgccgtc
      181 cttccgaagc tcatccatga aggtttccaa cttggggacg acgtccggat ccgcagagaa
      241 aaacggattc cgacccactg catcccaatc tttgaaagcc aactcatgcg gacccaactg
      301 gctcaaatag ccgcgaatca cgacaccata tttctcgctg agccactttc tcgcaatggc
      361 agcggcggca actcgaaccg cagtctcacg ggccgatgcg cgtccccccc ctcgatgatc
      421 acgaatacca tacttttgcc agtacgtgta gtctgcatgc cccggaccaa agctatcgat
      481 caaattccca taatcgcggc tgcgctgatc ttcattacgg atcaatagcg cgattggtgc
      541 gccggtggtc tgtccttcaa acaggcccga caggatttcc acgcggtccg actcctgccg
      601 ctgagtcacg tggcgagaag tgcccggttt tcgtcggtcg agatctttct ggatgtcttc
      661 ggtcgaaagc gaaagactcg gtggacaacc atcgatgacg cagccgatcg cagtcccgtg
      721 gctctcccca aacgatgtga cggtaaagat tctgccgaag gtattgcagg ccatgaggag
      781 aagagctccc tactcgacca gatccagctt gatgcgcaat tccttgagtt gatccggact
      841 cactgcagat ggcgcatcgg tgagcggaca ttgcgccttt tgagttttgg ggaaggcaat
      901 gacatcgcga atcgaatcgg cgttgccgag cagcatgacc aatcgatcca atccaaacgc
      961 aattcccccg tgtggcggcg ccccgtactc aagcgcatcg agcaagaatc caaacttagc
     1021 cgatgcttcc tccttgctga tcccgagaag gtcaaagact ttgctttgcg cgtcgcgccg
     1081 gtggatacga atgctgccgc cgccgatttc gctcccgttg agcaccatgt cgtaggcttt
     1141 ggcccgtacc tttagcggat cggactcgaa cagtgccagg tcttcatcaa gcggggcggt
     1201 gaatggatga tgcatggcca cataccgttt ctgctcgtga tcgtaatcca acaggggaaa
     1261 atcgatcacc cacaatggcc gccacgcgga ggtgtcgacc agcttcaact cttctcacaa
     1321 gagaagccga atccgcccca tcacatcgtg aacgaccgcc ggtttgtcgg caccgaataa
     1381 gaccagatca ccgggcttcg cttcaggcag agctgcggca aacgctttaa catccaagaa
     1441 tttggcaatc accgattcaa gctgcccttc cgccgtaatt ttgagccacg ccagaccctt
     1501 ggcgccgaag ctcttcgccg tttcgcccaa cgcgtcgatg cgcgtccgcg acagcgccgc
     1561 ccctcctttt acgattaagg ccttgacgat accgcccttg gtcgccgcat ccttgaagac
     1621 tttaaactca ctcgacgcgc caaatgccgt catatcgtag agtggcatgt cgaaccggag
     1681 atcaggctta tctgatccat atcggcccat ggcctccagg taggtcatcc gaggaaaggg
     1741 cgttgacaac tgaacaccgc cgaccgtact gaagatggtc acgatcatac gctccatgag
     1801 gctcatgatc tgctcgcgat caacgaacga catttccagg tcgatttggg taaattcggg
     1861 ctgccgatcg ttgcg
//
LOCUS       DATMTI010000877         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_797384, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000877 DATMTI010000000
VERSION     DATMTI010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797384"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..623
                     /locus_tag="VN039_13850"
     CDS             291..623
                     /locus_tag="VN039_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWV47080.1"
                     /translation="MMNDFVKVAFTHEIPPGTGRTVEVDGIWIAVFNVEGTFYAVDNS
                     CPHAGGPLGEGKLCEAVVECPWHGWKFSVISGERVGNPNFQVACCEVRIQGDEVWIAI
                     PPALREPA"
     gene            complement(620..1102)
                     /locus_tag="VN039_13855"
     CDS             complement(620..1102)
                     /locus_tag="VN039_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47081.1"
                     /translation="MADGSIFSRSPGVHLIRYAVAAILLLGGLYYAWTTLPSTTPSRD
                     KREAEALALVQSHPAIGHPTILQAMDAHVRTMKDRGQGVRLGEWRVKHVEGDIYEIRV
                     QLRDQGARGQWFEREFIWHAHLELKKVNAASLPADGVTPKAPDTDQQPAQQLTAPSQS
                     "
     gene            1298..1741
                     /locus_tag="VN039_13860"
     CDS             1298..1741
                     /locus_tag="VN039_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWV47082.1"
                     /translation="MKFFLYGDLLNPSQLKRRAPEHRFLYLATLSDHTVKFCRWSSQW
                     RCGLVSIVPSQGERTWGGVFEVTDEDVNIMDQFEQDVPQGAYRHLQVTVSAASGEKEL
                     VTTYAANPIGKFKPKEHYLDWVVKGLKQWKFPEEVIQEWESYRPR"
     gene            1744..2115
                     /locus_tag="VN039_13865"
     CDS             1744..2115
                     /locus_tag="VN039_13865"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWV47083.1"
                     /translation="MPSLHVPYRFFIGETMPKPKYHILVCTNSRPPGHPKPSCGSAGA
                     AQLLMAFNMGLMQRAVPPGEVLVSATGCLGPCEQGPTVVIYPDNTWYSKVTEADVVTI
                     LDEHVAKGTPAATLNPDSVWK"
BASE COUNT          499 a          628 c          657 g          480 t
ORIGIN      
        1 ggaaatccca tggatttccc tgtcgtacac ttccaagact tcgactttcc cttgccagag
       61 aagggcgatt gctttttgcc aatgcacgac ccgcaggggt tcataagtcg cgttgagcag
      121 gagggtcatt tccatgcagg aacctcatcc ccaatcgggc ctccacaacg gccagggagt
      181 gtttccgcgg ggcagggcta tacgctgata gttctatgcc ataaggtggc ggtgaaatca
      241 agcaagcgcc catctgatgc cgcatgcggc ggatcgatgg agagatgacc atgatgaacg
      301 atttcgtcaa agtggcattt acacacgaga ttccacccgg cacgggacga accgtcgaag
      361 tcgatgggat ttggattgcg gtcttcaacg tggagggcac tttttatgcg gtcgataact
      421 cctgtcccca cgcgggcggc ccgctcggag aagggaaact gtgcgaggcg gttgtcgaat
      481 gcccctggca tggctggaaa ttcagtgtca tttcgggaga gcgagtcggc aatccgaact
      541 tccaggtcgc ttgctgcgag gttcggatcc aaggagatga agtgtggatc gccatcccac
      601 ctgcactcag agagccggct taactttgcg atggagcggt cagctgctga gcaggttgtt
      661 ggtccgtatc gggcgctttt ggcgtcactc cgtcggccgg caaagaggct gcattgacct
      721 ttttgagttc gagatgcgca tgccagatga attctcgttc aaaccattga ccgcgcgccc
      781 cctgatcgcg aagctgcaca cggatctcgt agatgtcccc ctcaacatgc ttcactcgcc
      841 attcgcccag ccgaaccccc tggccgcgat ccttcatcgt acggacgtgc gcgtccatgg
      901 cctgcaagat tgtagggtgc ccgattgccg ggtgactctg caccaaagcc agcgcctcag
      961 cttcacgttt gtctctcgaa ggagttgtcg acggtaacgt agtccatgca tagtagagtc
     1021 cacccagcag caggattgca gcgactgcgt agcggatgag gtggacgccg ggcgatcgag
     1081 agaagatgct gccgtctgcc atgccgggtg ttgtctcagt gtaggagacc caaatcctgg
     1141 aaccgtgctg attgcacggc atcttaggaa ggcccggagg ccatgtcaat gcagcttcga
     1201 gaattggtcg tgcgaaggcc gcttgtctgg tgaaaagccg gtgtgttaca agtacgggca
     1261 cacgttaaga cggtcaggca caggatgagt acgctgcatg aagttctttt tgtacggtga
     1321 tctcctcaat ccctcacaac tcaaacgacg ggctccggaa caccgatttc tctatctcgc
     1381 gaccttgtcg gaccataccg tcaaattttg ccgatggtcg tcccagtggc ggtgcgggct
     1441 cgtcagcatc gtgccttcac aaggcgaaag gacctggggc ggagtgtttg aagtaacgga
     1501 cgaagacgtg aacatcatgg atcagtttga gcaggacgtg ccgcagggag cataccgcca
     1561 cctccaagtc accgtctcgg cagcgagcgg agaaaaagaa ctcgtcacta cctacgcagc
     1621 gaacccgatc gggaagttta agcccaaaga gcattacctg gactgggtgg tgaaagggct
     1681 caaacagtgg aagtttccgg aggaagtgat tcaggagtgg gagtcgtatc ggccgcgata
     1741 agcgtgccgt cgctacatgt tccatatcga ttttttattg gggagactat gccaaaacca
     1801 aaatatcata ttcttgtctg taccaattcc cgtccccccg gccacccgaa accgtcttgc
     1861 gggtcggccg gtgctgcgca gctgctcatg gccttcaaca tgggactgat gcaacgcgcg
     1921 gtcccgcccg gagaagtcct cgtgagcgcc acaggttgcc tcggcccctg cgagcagggc
     1981 ccgacggtcg tcatctatcc ggacaacacc tggtattcca aggtcaccga ggcggacgtc
     2041 gtcaccatcc ttgatgaaca tgtcgcgaaa ggaacaccgg cagcgacgct gaatccggac
     2101 tcggtgtgga aataaacgtc gtcaccgacg gcctgcttcg agcggccaga tgagactgac
     2161 gcttgattgc ttaaaccgat tactgatagc gtatcccttg gcacatgatc ataccaagtc
     2221 atcacgagca caaagaccat gcgcccacct caatcgggtg cctg
//
LOCUS       DATMTI010000878         1916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_584118, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000878 DATMTI010000000
VERSION     DATMTI010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584118"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(456..812)
                     /locus_tag="VN039_13870"
     CDS             complement(456..812)
                     /locus_tag="VN039_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47084.1"
                     /translation="MGQQRTDLLAIEFFDPVGAERPAGVTGEPALNAQQIGGSLPHHL
                     TTLAEEIPHRSIRAGINIAFGEYVQPQQVGQPARIAMIVGILESFILVDRRRIDEMNR
                     ITLLHESIDQPVPVIG"
     gene            959..1318
                     /locus_tag="VN039_13875"
     CDS             959..1318
                     /locus_tag="VN039_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWV47085.1"
                     /translation="MLALTIMLETGDIGRFDRVGHFASYCRCVGSEKISNGKKKGQGN
                     TKNGNTYLAWAFVEAAHFAVCYNTTIKRFYERKKAKTKMVVALKAVAHKLCRACYDMM
                     RNQVAFDLSKAFGTKAV"
BASE COUNT          451 a          516 c          537 g          412 t
ORIGIN      
        1 tccacagccg acgaagtttc acatcgactg caaacgccgc ttcaagaacc tcctcaactg
       61 ccacttcaaa tccgtccaga acctggttgg acagcaaatc caacagaata gacgcccccc
      121 tcccatgtgt tagctggaaa agattcgcga cactttttct gctctgcacg accgaacatg
      181 cttttctgcg ctatgcacaa tacaagatcc gataatcatc tagccagctt cttcgagagg
      241 cagtacggtt ggcggtgagc caacgtcaac actcagagaa ggaggcgacg aggaaatggg
      301 acgcagcgat cgatctacac tccagcaata atgtgacggt cgtgattgat gaacacgacc
      361 acatggtgta tcagaaacgc ttggcgaacg agctgacggt gatcctcacg gaactctcgg
      421 tctaccaggc cgagctgcag agcatcgcgg tcgagtcacc ctataactgg tactggctgg
      481 tcgatggatt catggagcaa ggttatacgg ttcatctcgt caatacggcg gcgatccacc
      541 aatatgaagg actcaagtat accgacgatc atagcgatgc gcgctggctg gcccacctgc
      601 tgcggttgga cgtactcccc gaaggctata tttatccccg cgcggatcga ccgatgcggg
      661 atctcctccg caagcgtagt cagatggtgc ggcagcgaac caccaatctg ttgagcattc
      721 agagctggct cacccgtaac accggccggt cgctcagcgc caaccgggtc aaagaactcg
      781 atggccaaca ggtcggtgcg ctgttgcccc atcgcgatct cgccctggcg gtcaaggcca
      841 atctatcggt gatgtcgagc gctgatgaac agaccgagat cttagagcga accattctgg
      901 aacgagtcaa gcttcgttca ccattcaaag tgctcaagtc tgtgccaggg atcgggatat
      961 tctcgcgctt accatcatgc tggagaccgg cgacatcggg cggtttgatc gcgtgggtca
     1021 ctttgcgtcg tactgccgct gtgtcggcag tgagaagatc agcaacggta agaaaaaagg
     1081 ccagggcaat accaagaacg gcaacacgta tttggcctgg gcctttgtcg aagccgccca
     1141 cttcgccgtg tgctacaaca ccacgatcaa acggttctat gagagaaaga aggccaagac
     1201 caagatggtt gtggcgctca aagcggtcgc gcacaagctg tgtcgagcgt gctacgacat
     1261 gatgcggaac caggtggcct ttgatctgag taaagccttt ggcacgaagg ccgtgtagac
     1321 gactgggatg gggctgtgag ctcgcattgg ggttggcatc aaccgacgag atctgattcg
     1381 ctgcagtccc atccctcacg gatcatccgc aaagggggtg atcgggtggg acgcccacgt
     1441 cgcgagccat ataggggttg gacccgaaag ggaaccaggg aatctgtgaa tctgttaccg
     1501 ctggaagcgg cgtggtaccg acggttttct ggggcacgtc cgagtgccag gggcggaacg
     1561 actacttagg gaagtagtcg tccaaggcct tgatcctgat gggtgactgg tgcggaccaa
     1621 cggtctgaca tcgtctgaaa cgaacatagg gatgcgtggc cgacccatca ccaccactga
     1681 cgagggggaa agaagaagat tctttttgcg ggaacgtctg tgccgacttg acctgccggc
     1741 taatgggcga ccccatttgt gtcttctgtg tgtgcgatat ttttcgaggt tgcatcacca
     1801 atgttcgttt ctgcgcttga cagtcaaaac agttgctgtc tccttttatt ccgcttcctc
     1861 ttgtccatcg attggtccct cataacaaga tatcgacaaa tcaatgttca gcttgc
//
LOCUS       DATMTI010000879         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_710969, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000879 DATMTI010000000
VERSION     DATMTI010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710969"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VN039_13880"
     CDS             <1..1722
                     /locus_tag="VN039_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HWV47086.1"
                     /translation="EGLISKQEAVQRIGADQLAQYLYPIFDTKEESKSNPLGKGLPAG
                     PGAAAGKISLNPDRAVELKAAGNRVVLVRQETSPDDIHGMNAAAGFLTARGGMTSHAA
                     VVARQMGKVCIAGCEAVEVLDSRTVKIGAKTFKEGDYLSINGSTGNVYEGDIPVVESE
                     IIQVIQGKLDPKQSDKYQRFATILSWADSFRKLRVRANADVPDQANIARGFGAEGIGL
                     CRTEHMFFAEDRIPIMQKMILARTKEEREKYLDQLLPLQKQDFVGLYREMKGYPVTIR
                     LLDPPLHEFLPKREELMVEIAQLELTGNDGAKLEEKRRLLARVEELHEFNPMLGLRGC
                     RLGITMPEITRMQARAIMEAACELAKEGAKIVPEIMIPLVGMVSEMKSQKDLLREVAQ
                     ETMKRCDVKLPYLIGTMIELPRAAVTAEHIAKEAEFFSFGTNDLTQTTFGFSRDDAAK
                     FIDHYRTVGIMESDPFAVLDREGVGSLMKQAVAGGRRTRSDIKLGICGEHGGDPNSIE
                     FCHELGLDYVSCSPYRVAIARLAAAHAALSEAEAKKSIKTTISSRMKPTRSQPRRRRS
                     RVTRKAR"
     gene            1749..>2555
                     /gene="ribD"
                     /locus_tag="VN039_13885"
     CDS             1749..>2555
                     /gene="ribD"
                     /locus_tag="VN039_13885"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWV47087.1"
                     /translation="MTLALRLAAKGRETTSPNPMVGAVVVGQGRIIGQGFHLRPGTPH
                     AEILALRQAGNRARGGTLYVTLEPCCHLKKRTPPCVPEILRSRVRRVVIAMQDPNPSV
                     QGKGAAALRRAGLSVTVGVARSEAEALNKSYCHWMKTGRPYVTLKAGMTLDGKLATAT
                     GESRWITGKASRREVHHLRRCVDGVLIGVDTVLVDDPSLTARTGPRLENLASRQPLRI
                     VVDSRLRTPFKAQILAQQDKAKTIIATTAAAPAARRSVLQKKGIEILTLPA"
BASE COUNT          585 a          672 c          785 g          513 t
ORIGIN      
        1 gaaggattga tctcaaaaca ggaagccgtg caacgcatcg gggccgacca actggcacag
       61 tatctctacc cgattttcga cacaaaagaa gagtcgaaat cgaacccgct cggcaagggg
      121 ttgccggccg ggcccggcgc agcggccggc aagatttcgc tcaaccctga ccgcgcggtt
      181 gagctgaagg cagccggtaa tcgagtcgtg ctcgtcaggc aagagaccag ccctgatgat
      241 attcatggga tgaatgcggc ggccggcttc ttaacggcgc gcggtgggat gacgtctcat
      301 gcagccgtgg tggcgaggca aatggggaag gtctgcatcg ccgggtgtga agccgttgaa
      361 gtgttggata gccggacagt gaagattgga gccaagacct tcaaagaagg agattatctc
      421 tcgatcaatg gatcgaccgg taatgtctat gagggcgata ttcctgtcgt cgaatccgag
      481 atcattcaag tgattcaagg gaagctcgat cccaagcagt cggacaagta tcaacggttc
      541 gccacgatct tgtcctgggc cgacagtttc cgcaagcttc gcgtccgtgc caacgccgat
      601 gtgccggacc aggcaaatat tgccagggga ttcggcgcgg agggcatcgg tctctgccgc
      661 accgaacaca tgttcttcgc cgaagatcgt atcccgatca tgcagaagat gatcttggcc
      721 cgaacgaaag aggagcgtga aaaatacctg gatcagttgt tgccgttaca gaaacaagac
      781 tttgtcgggc tctaccgaga gatgaagggc tatcctgtga cgattcgctt gcttgacccg
      841 ccgctccatg agtttctgcc caagcgggaa gagctgatgg tcgagatcgc gcagctggaa
      901 ttgacgggta atgacggtgc caagctggaa gaaaaacgac ggctcctcgc gcgagttgag
      961 gaactgcatg agtttaatcc gatgctcggg cttcgtggct gccgcttggg gattaccatg
     1021 ccggagatca cgcgcatgca ggcgagggcg atcatggagg ctgcctgcga attggcgaaa
     1081 gagggtgcga agatcgtacc tgaaatcatg attccactcg tgggcatggt ctccgagatg
     1141 aagtcgcaga aagatctgct ccgtgaagtg gctcaagaga cgatgaaacg ctgcgacgtc
     1201 aaactgccgt atctgatcgg gacgatgatc gaattgcctc gcgcagccgt gactgccgaa
     1261 catatcgcca aggaagccga attcttttct ttcgggacca atgatttgac gcaaacgaca
     1321 tttgggtttt cacgcgatga tgcggccaag ttcatcgatc attatcgaac cgtcggcatc
     1381 atggaatccg atccgtttgc cgtattggac agggaaggtg tcgggtcgct gatgaaacag
     1441 gcggttgcgg gaggccgtag gacgcgatcc gatatcaagc tgggcatatg cggtgagcat
     1501 ggaggtgatc caaactctat agagttctgt catgagctgg gcttggatta tgtgagctgt
     1561 tcgccgtacc gagtggcgat tgccagattg gcagcggcac atgccgctct gtcggaggca
     1621 gaggccaaga agtccattaa aaccactatt tcgtcacgaa tgaagccgac gagatcacag
     1681 ccacgtcgcc gtcggtcccg cgtgacccgc aaggcacggt gagtggtcac gctcgggact
     1741 tgcactacat gaccctggcc cttcgcctgg cggcgaaggg ccgggaaacg acaagcccga
     1801 atccgatggt cggagcggta gtggttggtc aaggcaggat catcgggcag ggttttcacc
     1861 tccgacccgg gactcctcac gcagaaattc tcgcgttacg ccaggcaggg aatcgcgccc
     1921 gaggtgggac tctctacgtc acgcttgagc cttgttgcca tctgaagaaa cgcactcccc
     1981 cttgcgtccc ggaaattctt cgctcacgcg tccgccgtgt ggtgattgcg atgcaggatc
     2041 cgaatccatc agtgcaggga aaaggagccg cagcgcttcg gcgggcagga ctctcggtta
     2101 cggttggggt tgcccgctct gaagcggaag cactgaacaa gtcgtattgc cattggatga
     2161 aaaccggccg cccctatgtg acgctcaagg ccggcatgac gcttgatgga aagctggcca
     2221 cggcaacagg ggagtctcga tggattacgg gcaaggcgtc tcgtcgggag gtgcatcacc
     2281 ttcgccgttg tgtcgatggc gtcttgatcg gagtagacac cgttctggtt gatgatccgt
     2341 cactgacagc gagaacagga ccgcgactcg aaaacttagc atcgcggcag cctcttcgca
     2401 ttgtggtcga tagccgattg cgcactccgt tcaaggcaca gatcctggca caacaggaca
     2461 aggccaaaac gattattgcc acaactgctg cagccccagc ggctcgacga tcggtcctgc
     2521 aaaaaaaggg catagagatc ctcacgttgc ccgcc
//
LOCUS       DATMTI010000880         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_203932, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000880 DATMTI010000000
VERSION     DATMTI010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203932"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..611
                     /locus_tag="VN039_13890"
     CDS             126..611
                     /locus_tag="VN039_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47088.1"
                     /translation="MKHSGHYFQHQTVGLIAMVVLLISSGCAQDSYQRRADVMKDHIE
                     NFYAHLRADRVEAAVHENEQIEVMADRMADTVRKQGQLQGTSQVGREFALMKTARDTA
                     AQNWIALGQYFAIKQQPERARASYRRVVDTYMHPTERAYREQAARALKDLEIVSGPAP
                     Q"
     gene            675..1211
                     /locus_tag="VN039_13895"
     CDS             675..1211
                     /locus_tag="VN039_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47089.1"
                     /translation="MIHSIAFKMLLLGLCVTWVGCVHRIQVTPLPASASSVTIPRTLQ
                     AVISPISMEGPDHRPGIVLLEWPHLDLKQAVLQYLQQRSTFASVSLDPADLTLRVATK
                     LTLTSRSDLYHYRIGLQAEMSEGVRSIKSYRAEHTAVGSSVRWVTASDRGPIEIALQA
                     ALEDLMRQVEVDRPIYHR"
     gene            complement(1252..1938)
                     /locus_tag="VN039_13900"
     CDS             complement(1252..1938)
                     /locus_tag="VN039_13900"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWV47090.1"
                     /translation="MTALMKIDQDSLPDRLVTGLSKIGLAMKSRAWRRKGRQGIGPLQ
                     IQVLTFLRSRPNHSATVSTIARELSVKLPTASEVIRTLEHKRLVRRRRREIDNRVVTV
                     HLTSLGAKAGHVENRWPEILASATENLSVQEQVALLTALVKLIRALQLQGEIPVARMC
                     VSCEHFHPHAYPESDQPHHCDFYNAAFGDQAFRLDCPEYVEAPAEGPSQNADSHKTAS
                     MTQTQGLTTV"
     gene            complement(2044..2901)
                     /locus_tag="VN039_13905"
     CDS             complement(2044..2901)
                     /locus_tag="VN039_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWV47091.1"
                     /translation="MKVLAATDGSKHGRWAIEWLAKMPFAVQPVVRVLHVVDLASLRA
                     PFMIQPMIVGTERYIQSEVKRMETAAKSTKKESEGLLSTAGLSGTVTIDRGGVAATIM
                     KHAQRGVGLLSIGSRGLDALDRFMLGSISNHAIHHALCSVLVVKEAPRPVRQVVLGVD
                     GSAASDKAVKFLMRYINPAPGGPNHEPVMVTVTHAVPYFKYPEVKEAGRTLVQRYGAK
                     LAKSGFQVREALRIGKPADEILTVAKQDKADLIVTGAKGLGAIRRVLLGSVSTRVVQH
                     AHCGVLVVR"
BASE COUNT          675 a          897 c          815 g          653 t
ORIGIN      
        1 ggcttcaccg gctcacccag actgcaccaa ggccgttcga gaattcccac cgatttggct
       61 agtgttcaga cgtatgttag actccccgag atgatatgct cgagaacttt cattcaagag
      121 gacccatgaa acattccggt cattacttcc agcaccaaac ggtcggtctt atcgccatgg
      181 tcgtcctcct catctcgtcc ggatgtgcac aagactcgta tcagcggcga gcggatgtca
      241 tgaaggacca catcgagaac ttctatgcgc acctcagagc cgacagagtg gaagcggcag
      301 ttcacgaaaa cgaacagatc gaagtcatgg ccgaccggat ggcggacacc gtcaggaagc
      361 agggtcagtt gcaggggacg tcgcaggtgg ggcgggagtt cgcgctgatg aaaacggccc
      421 gagacacggc ggctcagaac tggatcgcgt tgggacaata tttcgcaatc aagcagcagc
      481 cggagagagc ccgcgcctcc tatcgacgtg tggtcgatac ctatatgcac ccgacggaac
      541 gcgcctaccg ggaacaggct gctcgagcat tgaaagactt ggagatcgtg tcagggcccg
      601 ctccccagta gactcactcc tctcgcagat cgaaacattg tggcgagagt tcgtacgcca
      661 accgttgatt atctatgatc cactccattg cattcaaaat gctcttgctg ggcctctgtg
      721 tgacatgggt ggggtgtgtt catcgaattc aggtgactcc cctccccgcg agcgcgtcgt
      781 ccgttaccat ccctcgaact cttcaggccg tgatcagccc gatttcgatg gaaggtccgg
      841 accatcggcc aggcattgtc ttgctggaat ggccgcatct cgatctgaag caggcggtct
      901 tgcaatacct gcaacaacga agcaccttcg cttcagtctc gcttgaccca gccgacctga
      961 cgcttcgtgt ggccacgaaa ctcacgctga cctcccgcag cgatctctat cattatcgaa
     1021 tcgggttgca agcagaaatg agcgaaggcg tccgatctat taagtcgtac cgcgccgaac
     1081 acaccgcagt gggatcatcg gtacggtggg tcactgcttc cgaccgtggt cccatcgaaa
     1141 tcgcgttaca ggcggctttg gaagacttaa tgaggcaggt cgaagtggac cgaccgatct
     1201 accatcggta gaacagagca accgtcgcag aagttgcccg atgataaacc gtcagacggt
     1261 ggttaggcct tgagtttggg tcatgctcgc agttttgtga gagtcggcat tctggctcgg
     1321 accttccgcc ggagcttcca catactcggg gcaatcaaga cggaatgcct gatccccgaa
     1381 cgcagcattg tagaagtcgc agtgatgagg ttggtcggat tccgggtagg catgcggatg
     1441 gaaatgttcg caggagacgc acatgcgcgc aacaggaatt tcaccctgca attgaagcgc
     1501 gcggatcagc ttcacgagcg cggtcagaag agcgacctgc tcctgcaccg acaaattctc
     1561 ggtcgcagac gccagaatct ccggccatcg gttttccaca tgacctgcct tggccccaag
     1621 tgaggtgaga tgaacggtca cgacgcggtt gtcgatttcc cgacggcgcc ggcggaccaa
     1681 acgtttatgc tcaagcgttc ggatgacttc agaagcggta ggcagtttaa cggagagttc
     1741 tcgggcaatc gtcgagacgg tagccgaatg attcggtcga gaccggagaa acgtcagcac
     1801 ttgaatttgc aggggaccaa taccttgacg tcccttccgt ctccatgcgc ggctcttcat
     1861 cgccaaaccg atttttgaaa gccccgtcac taaacgatca ggaagtgagt cttgatcgat
     1921 tttcattaat gctgtcatat agcctctccc ttggagtatc cgagagggcg tcagtctatt
     1981 gcccctacct ccttgccgtc aagtcatttt aggctgccat gagccatccc ctacttaaga
     2041 tgatcagcgg accacgagca caccacagtg ggcatgttgc accacccggg tagaaacgct
     2101 gccgagtagg acacggcgga tcgcgcccaa ccccttcgcc ccggtcacga tcaggtccgc
     2161 cttatcctgt ttggctaccg tcagaatctc gtcagccggc ttcccgatcc tcaaagcttc
     2221 gcgtacctgg aagcctgatt tcgcgagttt agcaccatag cgctgtacca acgttctccc
     2281 ggcctctttg acctccgggt acttaaaata gggcactgca tgcgtcaccg tcaccatcac
     2341 tggttcatga ttcgggccgc ctggggccgg attgatatag cgcatcagga acttcaccgc
     2401 tttatccgat gctgccgacc catcgacccc caacaccacc tgcctgacag gccgaggggc
     2461 ctccttcaca accagtacgg aacaaagggc atggtggatg gcatggttcg agatgctgcc
     2521 cagcatgaat cgatccaagg catccaaacc tcgggacccg atcgacagga gtcctacacc
     2581 acgctgcgca tgcttcatga tcgtggctgc cacaccgcct cgatcgatcg tcacggttcc
     2641 gctcaagccg gctgtggaca ataaaccctc cgactctttt tttgtggatt tcgccgccgt
     2701 ttccatccgc ttcacttcgg actgaatata ccgttcggta ccgacgatca tcggttggat
     2761 cataaacgga gctcgaaggc tcgccaaatc gacgacatgc aggacacgga cgaccggctg
     2821 cacagcgaac ggcatcttcg ccaaccattc gatcgcccac ctaccatgtt tcgatccatc
     2881 ggttgccgca agaactttca tgtcggatta tcctttgttc gacgagatgc atcatattgc
     2941 ttagagggct gtgctgaacc gttctcgaaa tgcctctgga gacaggccgg ccaaggcggc
     3001 atagcggccc agcaggtccc gattgttcaa gtcggcttcc
//
LOCUS       DATMTI010000881         1867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_117248, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000881 DATMTI010000000
VERSION     DATMTI010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117248"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..892
                     /gene="hutC"
                     /locus_tag="VN039_13910"
     CDS             167..892
                     /gene="hutC"
                     /locus_tag="VN039_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HWV47092.1"
                     /translation="MTKASAGQTLHQRILSDIEGRIVSGEWPPGHRLPFEVDLADQYH
                     CSRMTVNKVMGQLAKAGLIERRKKSGSFVRQPQIQSAVLQIHDIGAEVESLGLEYGFK
                     ILERKGRSAKSGDRERLDVSVGTSLLEILCIHFAGAREFCVEQRLISLTAVPEAAETD
                     FTEIAPGPWLLGHVPWSTAEHRISAEAPSKEIARLLGISDRQACLVVERRTWSNSGAV
                     TYVRFTYPGNAHALVARFSPSSD"
     gene            1458..>1867
                     /locus_tag="VN039_13915"
     rRNA            1458..>1867
                     /locus_tag="VN039_13915"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          422 a          463 c          551 g          431 t
ORIGIN      
        1 cgtcgattcc gtttgggtgc gcggcaaaaa gcgcgtgcaa gccggccggc atatccaccg
       61 ccgtgaaata tccgatcgct tcaacaaggt catggtcgaa cttctcgata gctgatatgg
      121 gctcggcaga aacgggaaaa agccagtggg gaaaggcatt gcggacgtga cgaaagcatc
      181 ggccgggcaa acgctgcatc agcgcatcct cagcgacatc gagggccgga tcgtttcagg
      241 agaatggccg cctggccacc ggctgccttt cgaggtagac ctcgccgatc agtatcactg
      301 ttcgcgcatg acggtgaaca aggtcatggg ccagctcgcc aaggccggct tgattgagcg
      361 ccgcaagaaa tccggcagtt tcgttcgcca gccgcagatc cagtctgcgg tcctgcaaat
      421 tcatgacatc ggggcggaag ttgaatccct tggcctcgaa tatggcttca agatcctgga
      481 gcgcaaaggg cgcagcgcca aaagcggaga tcgtgagcgc ctcgacgttt ccgtcggcac
      541 gtcattgctc gagattctct gcatccattt cgccggtgcg cgcgaattct gtgtcgagca
      601 gcgtctcatc agtctgacgg cggtgccgga agcggctgaa accgacttta ccgaaattgc
      661 gccggggccc tggctgctcg gccatgtccc gtggagcact gccgaacatc ggatcagcgc
      721 cgaggcgccg tcgaaagaga tcgcacgcct gctcggcatc tccgacaggc aggcctgcct
      781 ggtggtcgaa cgtcgtacct ggagcaattc cggcgccgtg acctatgtgc gcttcaccta
      841 tcccggcaat gcgcatgccc ttgttgcccg tttcagcccg tcttcggact gatatccgca
      901 aggtcgcccg catcctttaa tcgtgaaatg gaaaccctat atagtgagag acttgtcctg
      961 ctttccgttc gcatggccaa aagtcgttaa aatccgttaa tctgtacgat aaatctcaaa
     1021 ttccctttta aaatcagcga tctgtcacat ttcttgattt ttttgatatt gctgtgttga
     1081 cttgtttgag ggtgtggatt tataagtccg ctcactgaac gagggcggcg gcgctgctgg
     1141 cgacgacgac tttcgttcta aagaaatcag gatgatgagc tgaatgcttg tttgtttcca
     1201 agggcttgat gcttttgggg tttgattttg tgactgtttg atgcggtctg ttttttgaca
     1261 attgaatatg agaagaaaga gaaacgtggg cggcgaagct tgcggggtct ggagagattt
     1321 ggaccctagt gaatagactt tgacggtcac gttttgaaca agagaataca cctcattatt
     1381 atcgcagcga tgcggaagat gatgggtgtg agttctcgtc gattcagaac gacgtgattt
     1441 agttaagatt gaattctcaa cttgagagtt tgatcctggc tcagaacgaa cgctggcggc
     1501 aggcttaaca catgcaagtc gaacgccccg caaggggagt ggcagacggg tgagtaacgc
     1561 gtgggaacat accctttcct gcggaatagc tccgggaaac tggaattaat accgcatacg
     1621 ccctacgggg gaaagattta tcggggaagg attggcccgc gttggattag ctagttggtg
     1681 gggtaaaggc ctaccaaggc gacgatccat agctggtctg agaggatgat cagccacatt
     1741 gggactgaga cacggcccaa actcctacgg gaggcagcag tggggaatat tggacaatgg
     1801 gcgcaagcct gatccagcca tgccgcgtga gtgatgaagg ccttagggtt gtaaagctct
     1861 ttcaccg
//
LOCUS       DATMTI010000882         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_246628, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000882 DATMTI010000000
VERSION     DATMTI010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246628"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..1336
                     /locus_tag="VN039_13920"
     CDS             374..1336
                     /locus_tag="VN039_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47093.1"
                     /translation="MSYRYNTIDIIVGVGMCAILFGGLLLFLAANGTYQVIQPQTFAL
                     EQQVGVDNGISFLQPALGQAIVDQALFERRTNQAVAQSVSEWNRATMAHHDLQSVSRG
                     LLGVTLKQAAIVPVDHLARVQGVMGRAIVNFTTRGVRHDLLSADQYGTMYNMKMIGVI
                     QAQGQRLHDQFTSTWQATLGHRIVEAAQHDWLRAGAIQERLGSALVQVVRAQMSSEEG
                     QAVQQEQLASLIFAAVRNEAITDRRTQPTVAVPSMVSSEDIAVASTEPMSWPEIPMTY
                     LIAAALLLATVFLGGLSMAAQSREAKALAQLRRDADRWAFRMAS"
     gene            1413..2132
                     /locus_tag="VN039_13925"
     CDS             1413..2132
                     /locus_tag="VN039_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV47094.1"
                     /translation="MKREISTSNRNQRNTAHRIDTGAGPRSIRLLIVEAQRLFRQSLR
                     VLLERERNIAAVVEATDGREAYRLAMEHKPDIVLLDVDMPDLDVESVTKLVRKHLPNT
                     RVLLLARYDEDSRIVAAMQAGAFGYILKDTDCTDFLRIIKATVSGEHILSPVIPERIV
                     RTVPGAVRQTHEDNNLLLSSLTDREREILACAAAGRGNKEIADQLCVSIDTVKTHLHH
                     IYQKLSVDGRVEAILTYLKTQ"
BASE COUNT          525 a          618 c          619 g          502 t
ORIGIN      
        1 aacatgaaaa tcaaacacag gacggcggaa cattttttca tgattccgta tgctacccat
       61 ctcggattat cccgccaatg aattctcgat ttggagcacg aaaggcacgc gacagaagga
      121 aagagtccct gcccgaagcc ccttgaccac actacccctc aagcgtagag tgaaatcttg
      181 aaccgcctca tcgttttgga ggtgggggcc atgcttgtcg cgggaatacc acagcgaccg
      241 actgagcatg gtaagtacgg cccccgctct ttttgctctc ttatcaatta tcccgctgag
      301 ttgtcttggg aaaatgttac cgggctcgtg agcccgacaa tgtgtaattg cctgatgtgg
      361 caaggaggtg gatatgtcgt accgctataa caccattgat atcatcgtag gcgtcgggat
      421 gtgtgcaatt ctctttggag gactcctgtt gtttctcgct gccaatggaa cctatcaagt
      481 aatccagcca cagacttttg cgttggagca acaggtaggc gttgataatg gcattagctt
      541 cctccagccg gccctgggtc aagccatcgt agatcaagcg ctcttcgagc gccgtaccaa
      601 tcaagccgtg gctcaatccg tctctgagtg gaatcgcgcg actatggcgc atcatgatct
      661 gcaatcggtc tcgcgcggct tgcttggagt caccctcaag caggcggcga tcgtcccggt
      721 ggatcattta gctcgcgtgc aaggtgtcat ggggcgagca atcgtcaact tcacgactcg
      781 cggcgtccgt catgatctct tgtcggccga tcagtatggc acgatgtaca acatgaagat
      841 gatcggcgtg atccaagccc aaggacagcg cctgcatgac caattcactt ctacttggca
      901 agccacgctc ggacaccgca ttgtcgaggc ggcccaacac gactggctcc gggcaggcgc
      961 aattcaggag cgactcgggt cggctctggt tcaggtagtt cgagcacaaa tgagctccga
     1021 agaagggcag gcggtgcagc aagagcagct ggcaagcttg atatttgctg ccgttcgcaa
     1081 cgaggccata acagatcgtc ggacgcagcc gactgtcgca gtaccatcga tggtgtcatc
     1141 agaagatatt gccgtggctt ctactgaacc tatgtcatgg ccggagatcc caatgaccta
     1201 cttgattgcc gctgctcttc tgttggccac cgtctttttg ggaggccttt ccatggcggc
     1261 gcaaagcaga gaagcgaagg cgcttgctca gctgagacgg gatgccgacc gctgggcgtt
     1321 tcgcatggcg agctaactga agtatgaacg gttacaccgg tgctcgacag aatcaggcgg
     1381 cgcgtgtcgg gcaccggtga gtcggattcc gtatgaagag ggaaatctct acgtcaaatc
     1441 gtaaccagag gaatacagcc catcgcatcg atactggagc aggaccccga tccatcagac
     1501 tgctcattgt cgaagcccaa cggctgttca ggcaaagtct gcgggtgctc ttggaacgag
     1561 aacgcaatat cgctgccgtg gtggaagcaa cggatgggcg cgaggcctac cgactagcga
     1621 tggaacacaa gcctgatatc gtcctcctgg acgtggatat gccggatctc gatgtggaat
     1681 cggtcacgaa actcgtccgc aaacacctcc ccaacacgcg agtgctgctg ctggctcggt
     1741 acgatgagga cagcagaatt gtcgcagcca tgcaggctgg agcgttcggc tacatcctga
     1801 aggataccga ttgtacggac ttcctgcgca tcatcaaagc caccgtgagc ggagaacaca
     1861 tcttgtcacc ggtcatcccc gaacgcatcg tccgcactgt tcccggtgcg gtgcggcaaa
     1921 cccatgagga caacaacctt ctgctttcca gtctgactga ccgtgaacgg gagatattgg
     1981 cttgcgcggc tgccggtcga ggcaacaagg aaattgccga ccagttgtgc gtttcgatcg
     2041 ataccgtgaa aacacatctg catcatattt atcaaaagct ctccgtcgac ggacgtgtcg
     2101 aagctattct cacctatctc aagactcagt gatctctttt tagccggcac tcactcaatc
     2161 ggtgtaggtc gcaattcact cttgtaggcg atagcaccga agcggtctac ctcttaacat
     2221 aaaagcaaat gctgaggttg gtccagacgc ctttacttgt aaag
//
LOCUS       DATMTI010000883         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_840615, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000883 DATMTI010000000
VERSION     DATMTI010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840615"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..493)
                     /locus_tag="VN039_13930"
     CDS             complement(296..493)
                     /locus_tag="VN039_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47095.1"
                     /translation="MAHPYFVAKHIGRNSPTSGQSPQSDSWAVNHIPPSIILAVSLYH
                     ITHPTPLRQYLPKGTGLSGST"
     gene            complement(506..1597)
                     /gene="modC"
                     /locus_tag="VN039_13935"
     CDS             complement(506..1597)
                     /gene="modC"
                     /locus_tag="VN039_13935"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HWV47096.1"
                     /translation="MSRLLARFDLRFPAFHLNVNLDIPMSGITAIFGPSGSGKTTLLR
                     CLAGLERVPHGFIQFGDDVWQDEEVGLCRPLYQRPVGYVFQEPRLFPHYNVRANLLYG
                     YTRIPSEERRIAMEQVVDILGIGHLLERRIHKLSGGEQQRVAIGRALLTSPKLLLLDE
                     PLASLDIQRKQELLPFIRRLHEELRIPAIYVSHAINEILQLADRVVLLREGKVVGIGV
                     LNEVFTSLKFRGSFGSHRVGAVLDTRVASHDSQYGLTQLEFKGKSLFVPLQSVSISEA
                     VRVHIFSSDVSLVVGQTNCPTSVLNILEATIVEIREMDQASVDVLLDVGTPLVASITR
                     KSLVNLDLKPGQRVFAHIKAVALNEELVE"
BASE COUNT          413 a          456 c          471 g          385 t
ORIGIN      
        1 gtcttccgtg ttcttgaaca cccatttccc gtccttctgc accagttccc catactcgac
       61 ggctttcgcc ctttctggct ctataagaag aggcacagtc agcaagacac accaaaacat
      121 cctcttcgac atgcattctc ccctttgttc tgattcaagc agtgcggggt ggttccaagt
      181 tccgagtgca acgggtgatt atggcgcacg tgcgcataga cttccaggat atagcaaggt
      241 tgaaacagtt gcgggtggtc gtgttcagcc gataggcgat ggcgggcaac ttctgttacg
      301 tggaacccga caggccggtg cccttcggca aatactgacg gagcggggtt gggtgcgtga
      361 tgtggtaaag tgaaaccgcc aggatgatgg aaggtggaat atggttgacg gcccaactgt
      421 ccgactgagg actttgtccg gatgtcgggc tattcctccc gatgtgtttc gcaacgaaat
      481 agggatgtgc catcggagac tctgtttact ccaccaattc ctcgtttaac gccacggctt
      541 tgatatgcgc gaagactcgc tgccctggtt tcagatcaag gttcacgagc gatttccggg
      601 tgatgctggc gacgagcggt gttccgacat ccaacagcac gtctaccgag gcctgatcca
      661 tctctcggat ctctacaatc gtggcctcca gaatgttcaa tacactggtc gggcaattgg
      721 tctggccgac gacgaggctg acatcactcg agaaaatatg gacacgcaca gcctcgctta
      781 ttgagaccga ctgaagcggt acgaacagag atttgccttt gaattcaagc tgagtcaggc
      841 cgtactgaga atcatggctt gccacgcgag tgtccaagac cgcaccgact cgatgggatc
      901 caaaacttcc acgaaatttc agggaagtga acacctcatt gagcacaccg atcccgacga
      961 ccttgccttc tctcaacagc acgacacggt cggcaagttg gaggatttca ttgatcgcat
     1021 gactcacgta tatcgccgga atgcgcagtt cctcatgaag acggcggatg aagggcagaa
     1081 gttcttgttt gcgctggata tcaagagaag cgagcggttc gtccagcaac aggagtttcg
     1141 ggctggtcag cagcgcccgg ccgatcgcta cccgctgttg ttctcctcct gacagcttgt
     1201 gaatgcgacg ttcaagaaga tggccgattc ccaagatatc gacgacctgt tccatagcaa
     1261 ttcggcgttc ttccgaagga atgcgtgtgt agccgtacag aagattcgcg cgtacgttgt
     1321 aatgagggaa caagcgcggt tcttggaaca catacccgac tggacgttga tagagtggtc
     1381 ggcataggcc gacctcttcg tcctgccaga catcgtcgcc gaactgtatg aagccatgcg
     1441 ggacccgttc cagcccggcg aggcatctca atagcgttgt ttttccggac ccggacggcc
     1501 cgaagatcgc ggttatgccc gacattggaa tatccaaatt gacgttcaag tgaaaggccg
     1561 gaaaacgcag atcaaagcgt gccagcaagc ggctcatgac gcatggatgg gaaatcggcg
     1621 attcgcgata tacacggtca acaacacgag aaacgaaaag atcagcataa aagccgagac
     1681 gacatgcgct tgggcatatt ctccgacttc gacatgctca aagat
//
LOCUS       DATMTI010000884         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_117367, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000884 DATMTI010000000
VERSION     DATMTI010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117367"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VN039_13940"
     CDS             complement(<1..812)
                     /locus_tag="VN039_13940"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV47097.1"
                     /translation="MAMSTVRRAQYWSHTVLRAAVVLIALLSLTGCLVRPHALNRDEI
                     KARAVKDFQAISSIEEPITKPIDLYEAIARALKYNLDAKVKAIQVQLAHQQLNIAHYS
                     LLPQVSANAGFDGRNNYSGGTGRSIITGRQAIEPFTSSEKNVTSGNLALSWDVLDFGL
                     SFVRAQQAADNVMIAEEEKRRIAVRLVQEVRNAYWGAVSAERVLPRIQFLNESVAKAL
                     DSAQLIVDRKLQAPLTPLTYQRDLLNIQREVRRLFRELSTAKTQLASIMGVPP"
     gene            complement(1026..>2209)
                     /locus_tag="VN039_13945"
     CDS             complement(1026..>2209)
                     /locus_tag="VN039_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HWV47098.1"
                     /translation="LMLAGLFFAPRLYRLNMLTVGDYYRYRYNRTVEVLCTLCIVASY
                     LGWVAAQFKVLGLVLNVVTEGSVSQPIGIVIGAAIVLTYTTFGGMFSVAILDFVQISV
                     IMGGLLYIASIVGDLAGGVRAVVNHAVQAGKLDLFPPATMTAWVPFVGAWMTMMLGSI
                     PQQDVFQRITSAKDEPTAVRGSLLGAALYFTFCFVPMFLAYAATLIDPVKFGALLERD
                     SQLVLPTLVLQHTPIAAQVIFFGAVLSAVMSCSSATLLAPSVALSENVIRPMFPHLND
                     SEFLRLMRVVLVGFASAVLILALWSDATIYKLVVSTYKVTLVAAFFPLFAGLYWKRAT
                     AQGALWAIVAGLTSWLALELVSRPTDVWPPQLVGFVMAGIGMLAGSLWPTQTREPQRP
                     S"
BASE COUNT          567 a          640 c          574 g          428 t
ORIGIN      
        1 ggtggcaccc ccattatcga agccagttgc gtcttggccg tactgagttc acgaaagagc
       61 cgccgaactt cccgctgtat gttgagcaga tcccgttggt aggtgagagg agtcagcgga
      121 gcttgaagct ttcggtcgac gatgagctgt gcgctatcca acgccttggc gaccgattca
      181 ttcaaaaact ggatccgggg gagcacccgt tccgcactca cggctcccca gtaagcattt
      241 cgaacttcct gtacgagccg aaccgcgata cgccgttttt cctcctcagc aatcatcaca
      301 ttgtcagcgg cctgctgcgc gcggacgaat gacaacccga aatccaggac atcccaactc
      361 aacgccagat taccggacgt gacattcttc tcggacgatg taaatggttc gatcgcttgg
      421 cgtccggtga tgatcgatcg ccctgtacca ccgctgtaat tattgcgccc atcgaaaccg
      481 gcattggcgg atacttgggg aagcaatgaa tagtgcgcga tattgagttg ctgatgagcc
      541 agctgaactt gtatcgcctt gaccttcgca tccaagttgt acttcaaggc acgtgcaatg
      601 gcctcgtaga gatcgatggg cttcgtgatt ggttcctcta tcgacgaaat ggcttgaaaa
      661 tctttcaccg ctcgagcctt tatctcatct ctgttcaatg catgtggtct gacaaggcag
      721 ccggtgagtg aaagcagagc gatcaaaaca acggcagccc gcaacactgt gtgagaccaa
      781 tattgggcac gccggacagt actcatcgcc atagcgggac cctgctccta agaaacttcc
      841 ggtaacgcta tcggctgagt tgaggaagaa cttgactcaa tggaagtgaa tagagccgaa
      901 ctatgctgag ctattgagga aacgggcatg gattcatagg tcttagagca ctttcgctcc
      961 cacgccatga agatgacggc ttgcctcgga ggggcatcaa atggcttctc gaagtatcga
     1021 ggccttcaac taggtctctg gggctcacgc gtctgggtgg gccacaagga ccccgcaagc
     1081 atgccgattc ccgccattac gaacccgacg agctgtggag gccagacgtc ggtaggccgg
     1141 cttactagtt ccaacgccag ccaactcgtg agcccggcga caatggccca caacgcaccc
     1201 tgtgccgttg cccgcttcca gtaaagtccc gcaaacaacg ggaaaaaggc cgctaccaag
     1261 gtcaccttgt acgtgctcac aaccagctta tagatggtcg cgtccgacca cagtgcaagg
     1321 atcaataccg ctgaagcaaa tcccaccaag accacccgca tgaggcgcag aaattccgaa
     1381 tcgttcagat gcgggaacat cggcctaatg acgttctcgc tcagcgcaac cgacggtgca
     1441 agcagggtcg cactggagca actcatcact gcagaaagaa ctgccccgaa gaaaataacc
     1501 tgcgccgcaa ttggagtatg ttgcaacacc agggtcggca agaccagctg cgaatcccgc
     1561 tccaacaatg ctccgaactt gacaggatca atcaaagtag cggcataggc gagaaacatt
     1621 ggtacaaaac agaaggtaaa atagagcgca gcgcccagca gcgaaccccg cacagctgtt
     1681 ggctcatcct ttgccgaggt gatccgctgg aacacatctt gttgtgggat ggaaccaagc
     1741 atcatggtca tccacgcccc gacgaatgga acccaagccg tcatcgtggc cggtggaaac
     1801 aagtcgagtt tgccggcttg aaccgcatgg ttgacgaccg cgcgcacacc gcccgccaga
     1861 tccccgacaa tcgacgcaat atacaaaagt cctcccatga tgacggagat ctgaacaaaa
     1921 tcgagaatcg ccacagagaa catgccgccg aacgtcgtat acgtcagaac aatcgctgcg
     1981 ccgatcacga ttccaatcgg ctggctgaca cttccctccg tcacgacatt gagcaccaat
     2041 cccagcactt tgaattgggc cgcgacccag cccaagtacg aggcaacgat gcagagcgtg
     2101 cacaatactt ctacggtccg gttgtagcga tatcgataat aatcccccac cgtcagcata
     2161 ttgagccggt acaggcgcgg ggcaaaaaaa agcccggcga gcatcaaac
//
LOCUS       DATMTI010000885         1564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_160694, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000885 DATMTI010000000
VERSION     DATMTI010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160694"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VN039_13950"
     CDS             <1..340
                     /locus_tag="VN039_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47099.1"
                     /translation="ELLIEATRAGHRSLAVPIEALRRPGARPSYFRPVLDIVRITCMV
                     GWKLLSSGMNPIGLYRSLCGPPSCGPSEPHGIKEVAPARCAHPTPPCSGTQSEVEEKE
                     ETAWQRMKCG"
     gene            complement(449..1387)
                     /locus_tag="VN039_13955"
     CDS             complement(449..1387)
                     /locus_tag="VN039_13955"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWV47100.1"
                     /translation="MQKKVEQLETHNQHGASVQAPSEQSNGSPGRRHMPARTCSQWHF
                     TIDVDWVPGSEVGLEGLLDFCDRRRLKATIFVAGRFAENYADLIRECRRRGHELGTHG
                     WEHGSLENDEDFRSASYDQQRAWIRLATEAVEKASGVRPVVFRAPSLWVGETTLRALE
                     DEGYRYDSSVPARRFDCGFGRVHYLKYFRAPTEPYRLSSSHLRLPGHSSIVEMAPSAI
                     LFPINMATLRVLGLQAMKWMVRWISRRTSRIVFYCHPHEFVHARRQTFPRNMSTWNMQ
                     GMRPENFTLLDAFVEHMMHLGYSPALFAESFGKTLA"
BASE COUNT          309 a          478 c          436 g          341 t
ORIGIN      
        1 cgagctactg atcgaggcca cgcgtgcagg gcatcgaagc ctggcagttc cgattgaagc
       61 cttgcgccgt ccgggagcgc gaccgagcta ttttcgtccg gtgctcgata tcgtccgcat
      121 cacctgcatg gtgggatgga aacttttgtc cagcggtatg aacccgatcg gtctctaccg
      181 ctcgctctgt ggcccaccct catgcgggcc gagcgagcct cacggcatta aggaggtcgc
      241 gccggctcga tgcgcacacc ccacacctcc gtgtagcggg acccaaagtg aagttgagga
      301 gaaggaagaa actgcctggc agcgtatgaa atgcgggtga agtccgctgc gagacacttc
      361 agcctgcgat agccgccccg caatgctcgc cccacaagtt gtatgacggt tggtcgcgca
      421 ccggcgagcg agcgattgat tcatactatc aagcaagggt cttgccgaac gactccgcaa
      481 acagggcggg ggaatatcct agatgcatca tgtgttcgac gaatgcgtca agcagggtga
      541 agttctccgg cctcatgccc tgcatattcc atgtggacat gttgcgtggg aaggtttgcc
      601 gacgcgcatg cacgaattcg tgggggtggc aatagaacac tattcgcgaa gtccgtcggg
      661 agatccagcg gaccatccac ttcatcgcct gcaggccgag cacccggaga gtggccatgt
      721 tgatagggaa caggatcgct gagggggcca tctcgacgat cgagctgtgt cccggcaggc
      781 gaaggtggct tgacgagagg cggtatggct cagtcggggc acgaaagtat ttcaggtaat
      841 gcacccggcc gaagccgcaa tcaaatcgtc tcgccggcac cgatgaatca tagcggtatc
      901 cctcgtcttc gagcgcacgc aaggtcgttt cgccgaccca caggctcggt gcgcggaaga
      961 cgacggggcg aacccctgag gctttctcca ctgcctccgt ggccagccgg atccatgctc
     1021 gctgttggtc atagctggct gatctgaaat cctcatcatt ctcgaggctg ccgtgttccc
     1081 acccatgcgt accgagttca tgccctcgcc gccgacactc cctgatcaaa tccgcataat
     1141 tttccgcaaa ccgcccggct acaaaaatcg tggccttgag cctccgtcga tcacagaaat
     1201 ccaacaaccc ttccagtccg acctcggacc cagggaccca gtccacatcg atggtgaaat
     1261 gccactgcga gcaggttcgt gcgggcatgt gccggcggcc tggcgatcca ttggattgct
     1321 cagagggagc ctgtaccgaa gccccgtgct gattgtgcgt ttccagttgt tcgaccttct
     1381 tttgcatttc ggacttcgcg ttgtctgacg cgttgcttgc cttccccttc aattggtcac
     1441 ttttggcttg cctctcttcc agctcaaccc gcatcctttt atacaagatc tctttcttct
     1501 ataccctatc ttccccatgt cgggaacttc cttgatctcc attccgatcg ccctcagcag
     1561 tgac
//
LOCUS       DATMTI010000886         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_204103, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000886 DATMTI010000000
VERSION     DATMTI010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204103"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..534
                     /locus_tag="VN039_13960"
     CDS             109..534
                     /locus_tag="VN039_13960"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HWV47101.1"
                     /translation="MRKERSISKNIIVCTDGTWDHPDSKTPNVGTTPDETNVFKFFDL
                     LPGEAQIRPAGVRVKSIQGQTTFYDDGVGADGIWAVRIAEGATGAGLELKLQAGYRFI
                     SEQYEDGDRIYLFGFSRGAYTARSIGGMLTNAACRRDND"
     gene            513..902
                     /locus_tag="VN039_13965"
     CDS             513..902
                     /locus_tag="VN039_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HWV47102.1"
                     /translation="MPARQRLNDIFASHAFDVYRRNNDTITAQFRKDYQSRDVLTEVI
                     GVWDTVGALGIPLAFFSGLDHLLFSFHDTSLHPNVRFGYHAVAIDESGKAFRPPCGTR
                     AKAWSRCGSPESIATSAAVIKKRACPT"
     gene            926..1177
                     /locus_tag="VN039_13970"
     CDS             926..1177
                     /locus_tag="VN039_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47103.1"
                     /translation="MQPHGLLFKDGTFTKDGTATITGDPLMEPMHDSYKPPFITLHPS
                     VRVISLSATLHVSAQERLEKAKPEYRPVNLPPEPRKYVE"
     gene            1766..2932
                     /locus_tag="VN039_13975"
     CDS             1766..2932
                     /locus_tag="VN039_13975"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWV47104.1"
                     /translation="MHGFTTRQLHADGQMKPLNAHTMPIFLTSTFAFDSPEAGADLFL
                     GRRAGHVYSRMGNPTVETVEQVVADLENGEAAVAFGSGMAAIHASLLSVLKAGDHMIC
                     GEALYSPSLHLITQRLADLGITSSVVDTSDVRAVEGSIRDNTKVIFYETPANPTCKIT
                     DIRAIAELARSKDVLTIVDNTFSSPYFQRPLDLGTDISLHSATKYLNGHGDVIGGIVV
                     ARSELAAKIRTYRRDTGGILSPFEAYLLLRGIRTLSLRMQRHHDNAMKLFEFLAGLPT
                     VSTLYYPGDPKFPGHHVAARQMTGFGGCFSFELAGGFEAAKRLLKRLTLCTLAVSLGT
                     VDTLIEHPASMTHVGMPADVMARQGLTSGLVRISVGCEDIEDVIGDLENALKTD"
     gene            2989..3891
                     /locus_tag="VN039_13980"
     CDS             2989..3891
                     /locus_tag="VN039_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47105.1"
                     /translation="MQEIAFILRPVQPFRLDLTVWTLRRRPNNMMDRWEEGVYRRTLV
                     VDAMPVQVAVTQQNSTLAVTVAGVGLSSRTQADVTAALERLLGLRIDLRTFYRLASQE
                     TKLHQLAKRFLGMKPPRFPSVFEALVNAIACQQLSVTVGITLLNRLTERYGLGSRSED
                     RAFPRPDDLSKVDVQALRALGYSRSKGQAVIEAARAMRESALDPDSFATMDDTETINR
                     LMELRGVGRWTAEYTLLRGLGRIHQFPGDDVGAQKSLQRWLQLKRPPDYDHVRRVLRK
                     WRPYAGLIYFHLLLDGLDRRGWLA"
BASE COUNT          861 a         1074 c         1164 g          813 t
ORIGIN      
        1 ggcattgctc gtgaatgggt gaaatgggga caaggctact tttgacccag ccggaggagc
       61 cctgtaggat tccaacattc tgattctgac tgttgctcca gcagttgatt gaggaaggag
      121 aggtctatat ccaagaacat catcgtctgc accgacggca cctgggacca tccggattcc
      181 aagactccga acgtcggcac gacaccggat gaaacgaatg tcttcaagtt ctttgacctg
      241 ctgccgggcg aggcgcaaat ccgcccggcc ggggtgcgcg ttaaatcgat tcaaggacaa
      301 accacctttt acgacgacgg cgtcggcgcc gatggcatct gggcggtacg aatcgccgaa
      361 ggggcgacgg gagccggttt ggagctcaag ctgcaagctg gatatcgatt cataagcgaa
      421 cagtatgagg atggcgatcg gatttatctg tttgggttca gcagaggcgc ctacaccgcc
      481 cgcagcatcg ggggaatgtt gaccaatgcg gcgtgccggc gcgacaacga ctgaacgata
      541 tcttcgcaag ccatgccttc gacgtctacc gccggaacaa tgatacgatc acggcccaat
      601 tccgcaaaga ttatcaaagc cgagacgtgc tgaccgaagt catcggcgtg tgggacacgg
      661 tgggcgcgct cggtattccc ctcgcattct tcagcggcct cgatcatcta ttgttcagtt
      721 ttcacgatac ctcgctgcat cctaacgtcc gcttcggcta ccatgccgtc gccatcgatg
      781 aaagcgggaa agctttccgc ccaccctgtg ggacccgcgc aaaggcgtgg agcaggtgtg
      841 gttcgccgga gtccattgcg acatcggcgg cggttataaa gaaacgggcc tgtccgacgt
      901 gacgttcgga tggatgttga acaggatgca accccatggc ctgctgttca aggacggcac
      961 gttcaccaag gatggaacgg ccacgatcac cggcgatccc ctcatggagc cgatgcatga
     1021 ttcctacaaa ccgccgttca tcacgcttca cccttccgtg cgcgtgattt ctctctccgc
     1081 cacgttgcat gtcagcgcgc aagaacggct ggagaaggcg aaaccggaat accggccggt
     1141 caacctaccg ccggaaccgc gcaaatacgt tgagtgatca cgcctcgccg atagtattca
     1201 gcaggtcacc cagctcccgg caagcatcga ccttttcttc agctaccagc ttcatgatcg
     1261 taacagggcc gcagcagctt ctcgggagtc atcgaggaca aagcacgaac gatccccatg
     1321 acgtcacgtt caggacacta ggtgttttgc gaggtgtgtt agagggatga gacttgcttc
     1381 actatgtcgt gctgaatagt ggtcgaaatt attcgacggt gaatgaatgt tggagtttcg
     1441 cgagaaccgg acgacagatc gccgttagaa gtagaagcat gctccacagg atgggacaaa
     1501 tcatttcatt tcggcgagca gatacgcatg tgacgtgcag tgcgcaagta ctcgaaccaa
     1561 gccataagaa atgcgcgccg ggagattcga tcatggagaa agagagcagt gtggtcgttc
     1621 aactcgggat gccccctcgc tacttggacc ctcggtgcca cgagaggctc tatgaggtcg
     1681 acatgggagt caatgagcca tatgattgag cagacgctga ctcagcgctc acgaagaggg
     1741 aacagcacgg acaaaggagc ccttcatgca cggatttacg accagacaac tgcacgccga
     1801 cggacagatg aaacccttga acgcccatac gatgccgatt tttctcacgt ctacgtttgc
     1861 gttcgattcc cctgaagccg gtgcggatct gtttctggga cgtcgcgccg ggcacgtcta
     1921 cagccggatg ggaaatccga cggtcgaaac cgtcgagcaa gtggtcgctg atctggaaaa
     1981 cggagaggcc gccgttgcct ttgggtcggg gatggcagcc atccatgcca gtttgctgag
     2041 tgttttgaaa gcgggcgatc acatgatctg cggggaagcg ctgtatagtc ccagcctcca
     2101 tttgatcaca caacgtttgg ccgatctggg gatcacgtcg tcagtcgtcg atacctctga
     2161 tgtgcgggcc gtcgagggct ccattcggga caataccaaa gtgattttct acgagactcc
     2221 cgccaatcct acctgcaaga tcaccgatat ccgggccatc gccgaactgg ccagatccaa
     2281 agacgttttg acgatcgtcg ataacacctt ctccagcccc tattttcaac ggccgctgga
     2341 tctcggcacg gacatctctc tccatagtgc gacgaaatat ctgaacgggc acggcgacgt
     2401 gatcggaggg atcgtggtgg ctcgctctga gctagccgcc aagattcgta cgtatcgccg
     2461 cgacaccggc ggcattctca gtccgttcga ggcctatctg ctcttgcggg gcatcagaac
     2521 cctttccttg cgcatgcaac gacatcatga caacgcgatg aagcttttcg aatttcttgc
     2581 cggtcttccg acagtcagca cgttgtacta tcccggcgat cccaagttcc ccggccacca
     2641 tgtggcagcc cgtcagatga ccggctttgg cggttgcttc agtttcgagc tggccggcgg
     2701 gttcgaggca gccaagcggc tgttgaagag actgacactt tgcacattgg cggtgtcctt
     2761 gggcacagtt gatacattga tcgaacatcc ggcgtcgatg acccatgtcg ggatgccggc
     2821 agacgtcatg gcacggcaag gactcacgag cgggctcgtg agaatctcgg tcggctgcga
     2881 ggatatagaa gatgtgatcg gcgatctgga aaatgcgctg aaaacggact aagcgcctgc
     2941 acgagccatg caccgatcgg ctgatcgcgg tgaggccgcg cctgaaccat gcaggagatc
     3001 gctttcatac tgcgtccggt gcagcccttc cggcttgatc tcaccgtctg gacattgcga
     3061 cggcgcccga acaatatgat ggatcgttgg gaggaagggg tttacaggcg gaccctggtc
     3121 gtcgatgcca tgccggttca ggtggccgtc acacagcaga actccacgct ggccgtcacc
     3181 gtcgctggtg tcggtctgtc gtcgaggacg caagccgacg tgacggctgc attggagagg
     3241 ttgttaggcc tccggataga tcttcgcaca ttttatcggc tggcgtcgca ggaaacaaag
     3301 ctgcatcaat tggccaagcg ttttctcggg atgaagccgc cgcgtttccc tagtgtattt
     3361 gaagcattgg tcaacgcgat cgcctgccag caactcagcg tgactgtcgg gatcaccctg
     3421 ttgaatcgct tgacggagcg ctatgggtta gggagccgca gcgaagaccg tgcgtttcca
     3481 cgtcccgatg atctgagcaa ggtcgacgtc caagcgctga gagcattggg atatagccga
     3541 agcaagggac aggccgtcat tgaggcagcc cgagcgatga gggaaagcgc gctcgacccc
     3601 gatagcttcg caaccatgga tgataccgag acaataaacc ggttgatgga gttgcgagga
     3661 gtggggcgtt ggaccgctga atatacgctg ctgcgtggcc tgggacggat acaccagttt
     3721 ccgggcgatg atgtcggggc ccagaagagt ctccagcgat ggttgcagct gaaacgaccg
     3781 ccggactatg accatgtccg ccgggtgctg cgcaagtggc gaccgtatgc cgggttgatt
     3841 tactttcatt tgttgctgga cgggcttgac cgaagggggt ggctggcatg agccagcacc
     3901 gaaagtaatc gg
//
LOCUS       DATMTI010000887         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_246754, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000887 DATMTI010000000
VERSION     DATMTI010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246754"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1430)
                     /locus_tag="VN039_13985"
     CDS             complement(63..1430)
                     /locus_tag="VN039_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWV47106.1"
                     /translation="MVNERRLRSFVKESRPRFEDLLGQMVEVPSISMDATRSGDMQRM
                     ADLAGQYLAGMNAEVQLVETGGYPIVSGGWITGPECPTVTIYNHLDVQPAQEPEWRQA
                     PFVFKNENGIYRGRGATDDKGPALTAMFGARYAIEQGWPINIRFLWELEEEIGSPHFG
                     VGLKNRSSIPRPDSVVVSDTIWIAKGRPAAPYGLRGLLGARLCLHTGSKDAHSGVTGG
                     AARNPLAELMEVANTCVDARTGRVKISGFYDDVIEPTKREIKNFLTSGFQIRRFKEAY
                     GFRSLRVQDPAEVMRRIWASPTFEIHGLSGGYHGQGIKTVVPGHAELKISMRLVPNQV
                     PEKVFALLKKHVAKVNPDVKVEKAGTLHPFKGSFAGPYVDCAKRAIKAGFGKDPAFVR
                     EGGSIGAVVTMQKTWKVPILFIGLSLPEHGYHAPNEYYDWGQASGGMKTFAHYFWELA
                     NMRKT"
     gene            1543..2010
                     /locus_tag="VN039_13990"
     CDS             1543..2010
                     /locus_tag="VN039_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47107.1"
                     /translation="MPTSTRIVAPLFAVLLTLIGANPPILVWAEESTNIQQILEEPRV
                     YHLRHVTLQGTVRDVQPLAPYTVPNGDRCYGAYLFRLEEDTATIPVAVLGICGRPIVR
                     DPEIEDGDRVEVSATIQAPSHGGYYLSFKGLKVVTEQEGVVQAVADRILPIVE"
     gene            2131..2838
                     /locus_tag="VN039_13995"
     CDS             2131..2838
                     /locus_tag="VN039_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47108.1"
                     /translation="METRGLCLTIVLALIVTVSAGCASSQGFNRAAMFETLHLDSSTE
                     EDGQPIANQVSRFSSPFRLGVFFVDHNIPPGQSIRKVEWLSADRGQLLHDLAPLQGDH
                     ILMDTFVLMDATLRGEDIRAIRQAGARYGADLVLIIDGVAAIDRYNNRSAWWYPTLIG
                     AYLARGTESHALVMTTGILWAVRSEWHAPLQRAEEAAKLVGPAAFVEDTAALQEARKR
                     AIEAISKRIADQLRLLK"
     gene            complement(2860..>3428)
                     /locus_tag="VN039_14000"
     CDS             complement(2860..>3428)
                     /locus_tag="VN039_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWV47109.1"
                     /translation="FHLSHANNLPSYGGRWAWKATPRLTLTQTLYAGPDQTNTSLEFW
                     RLYGNHVLEWRSKDVAVAASFDVGTENIANRPGNPRAFVMGGNIVVRWHIAGPWAVAL
                     RPEFYWDRNGRWTGAEQFVKAITSTIEYKLPYKWMNTVTRIEHRYDESTGVGGGFFKD
                     GGLRPGEVSLTPGQHLLLLGVLLSFDSP"
BASE COUNT          753 a         1003 c          874 g          798 t
ORIGIN      
        1 catgttcaaa actgatcttt cgtcatttcc atgggttgtc aaaaaaatag ccaataccga
       61 tcctatgtct tcctcatatt ggctaactcc cagaagtaat gagcgaatgt cttcattcct
      121 cctgaggcct ggccccagtc gtaatattcg ttgggggcgt gataaccatg ctcgggcagg
      181 ctcaaaccta tgaaaaggat tggtactttc caggttttct gcatcgtgac caccgcaccg
      241 attgatcccc cttctcgcac aaatgcggga tctttgccaa atcctgcctt aatggcgcgt
      301 ttcgcacaat ccacgtatgg ccctgcgaag ctgcccttga atggatggag cgtgcctgcc
      361 ttctccacct tcacatccgg attcaccttc gccacatgct tcttcaacag cgcaaacacc
      421 ttctccggta cctggtttgg gacaaggcgc atactgatct tgagctccgc atggccgggc
      481 acaaccgttt tgataccttg tccgtgatat cctccgctga gaccatgaat ttcgaaggtc
      541 ggagatgccc agatccgtcg catgacttct gccgggtctt gcactcgaag agatcgaaac
      601 ccgtaggcct ctttgaatcg tctgatttga aagccggacg tgaggaaatt cttgatctct
      661 ctcttcgtgg gctcaatcac gtcgtcgtag aaacccgata tctttaccct gccggtcctg
      721 gcatcaacac aagtgttcgc cacctccatc aactcggcca gcgggttacg agccgctcct
      781 cccgtcacac ccgagtgtgc atctttggac ccggtatgta ggcagagtcg tgcgccgagc
      841 aggccgcgca agccgtatgg cgcagcaggc cgccccttgg caatccagat cgtatccgac
      901 acaaccacgg aatccggtcg tggaatagag ctccggtttt taagcccgac gccaaaatgc
      961 gggctcccga tctcttcttc caattcccac aagaatcgga tgttgatcgg ccaaccctgt
     1021 tcgatagcat atcgagcgcc aaacattgcg gtgagagccg gccccttatc atcggtcgct
     1081 cctcgccccc gataaattcc gttttcattc ttaaaaacaa acggcgcctg cctccattcc
     1141 ggctcctgag ccggctgcac gtccagatga ttgtagatcg tgacagtcgg acactcaggc
     1201 cctgttatcc acccgccgga gacaatgggg tacccaccgg tttctaccaa ttgaacctcg
     1261 gcattcatcc cggccaagta ctgccccgca aggtcggcca tgcgttgcat atcgcccgaa
     1321 cgggttgcat ccatactgat cgacggcacc tccaccatct gtcccaaaag atcctcaaac
     1381 cttggacgtg actcctttac aaaactcctc agtcgccgct cattcaccat acgaatgatc
     1441 ctccttgtcc ataagacggg ggattatagc gatcgcgcct ggcaagagaa agcagctccg
     1501 ggtttcaaac gcagaacaat gataagatgc ctttcatcca ccatgcccac ttcaacgcga
     1561 atcgtcgctc ctctcttcgc cgttctgctc acactgatag gagcaaatcc accgatactg
     1621 gtgtgggcgg aggaatcgac caatatccag cagatacttg aagagccacg ggtctaccat
     1681 ctacggcatg tcactttgca gggcacagtg cgtgacgtgc aacctttggc gccctacaca
     1741 gtaccaaacg gagatcgttg ttatggggcc tacttgtttc gtctcgaaga ggacactgct
     1801 actatcccgg tggcagtatt gggtatttgt ggcagaccaa ttgtccgtga tccggaaatc
     1861 gaggatggag atcgtgtcga agtcagcgcc acgattcagg caccgagtca tggaggatac
     1921 tatctcagtt tcaaaggatt gaaagtggtg accgagcagg aaggagtcgt gcaggcagtg
     1981 gccgatcgca ttctcccgat cgtggagtag atccttctct ccaatacatc tacaggcatg
     2041 ctatggagtt gccgtgtcac tggatcatgc gacaggaatc ttgtcgaatt catcatcatt
     2101 cggtttggag atggaagcgg actcttttgt atggagacta gagggctatg cctcacgatc
     2161 gtcctcgcgc tgattgtgac agtatcggcc ggctgtgcga gcagccaagg gttcaatcgg
     2221 gctgcgatgt ttgagactct gcacttagat tcctccacag aggaagacgg ccaacctatt
     2281 gcaaaccaag tctctcgctt ttcctcgccg tttcgccttg gtgtcttttt cgtggatcac
     2341 aacatcccgc caggacagtc catcaggaaa gtagaatggc taagcgcgga tcgaggccaa
     2401 cttctccatg accttgcacc attgcagggt gatcacatac tcatggatac gtttgtgctg
     2461 atggacgcca ctctgcgagg agaggacatt cgcgcaatcc gccaggcggg tgcgcgctat
     2521 ggggccgatc tggtcttgat cattgacgga gtagccgcca tcgatcggta caacaaccgc
     2581 tctgcgtggt ggtatcccac cttgatcggc gcctatttgg cgcgtggtac cgaaagccat
     2641 gcgcttgtta tgaccaccgg catcttgtgg gccgtgcgct ccgaatggca cgctcccctc
     2701 cagagagccg aagaagcagc aaagcttgtc ggaccagcgg ccttcgtcga agacaccgcc
     2761 gcgctgcagg aggcaaggaa gcgcgccatc gaagctatca gcaagcgaat cgccgatcag
     2821 ctgcggctcc tgaaataact gcaccgcatg atacttccat tacggtgagt cgaagctcaa
     2881 gaggactccc aacaacagca gatgctgccc cggtgtaaga ctgacttcac cgggacggag
     2941 cccgccgtct ttgaaaaatc cgccaccgac tcccgtggat tcatcgtaac ggtgttctat
     3001 ccgcgtcaca gtgttcatcc atttatatgg gagcttgtac tcgatggtgg acgtgatggc
     3061 tttcacgaat tgctcggctc cggtccagcg cccatttcgg tcccagtaga actcgggtcg
     3121 cagggccacg gcccaggggc ccgcgatatg ccatctcact actatgttgc cgcccatgac
     3181 gaaggctcgt ggattgcccg gtcgattagc aatgttttcg gttccgacat caaaagacgc
     3241 cgcgaccgcg acgtctttgc ttctccattc gaggacatga ttcccgtaca agcgccagaa
     3301 ctccagggaa gtattcgtct ggtccggtcc tgcatagagg gtttgcgtca acgtcaacct
     3361 gggcgtagct ttccaggccc atcttccgcc gtagcttggc agattgttcg catgcgacag
     3421 atggaagt
//
LOCUS       DATMTI010000888         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_754638, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000888 DATMTI010000000
VERSION     DATMTI010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754638"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VN039_14005"
     CDS             complement(<1..312)
                     /locus_tag="VN039_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47110.1"
                     /translation="MTTVAASQVPKLQFFNPSTGVPLAGGLLFTYAAGTTTKQVTYSN
                     PDNLTPNTNPIVLDSTGSCVCFLDATLQYEFTLSPPGDTDPPTDPIYSVDQVGYADIL
                     QG"
     gene            complement(309..1640)
                     /locus_tag="VN039_14010"
     CDS             complement(309..1640)
                     /locus_tag="VN039_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47111.1"
                     /translation="MRVLLIDTDRCGLDFAYRCVQAGHDVRWYNRSVDGEAFKDGQGF
                     PGIAMVKDWRESMKWAKDGLIWTSSNCKFLKELDQWKQHGFPVFAPSFESAQLEISRS
                     KGMDVLKKCGMDIPPYTMFDSLDAALKFAWKATQPYVFKTMGDCEDKSLSYVAKTPAD
                     LVGRIQRWIKDGLKVKGPCMLQERVDGVEFAVSCWFGPEGPLPEKWNINFEHKKLMPG
                     NYGPNTGEMGTVIQYTKQSKLADAVFTPKLVAALRKLRHLGDVDVNCIVDKEGTPWPL
                     EFTCRPGWPFDWIVDETHPEDPAEWKRDLLLGKDSLNVSYDVAIGNVIATPPFPYDEQ
                     DDGQCMGLPVQTPKNWRGIHPVNMMLDKGPAMEGEKVVEKDIFKTTGDYVMVCAGTGK
                     TVMAAKKATDKIIGQVQVPNMIVRNDIGMGLEKVLPTLHKLGYAENMEFDA"
BASE COUNT          324 a          523 c          497 g          398 t
ORIGIN      
        1 cccttgcaag atgtccgcat aacccacttg atccacgcta tagatcggat ccgtgggcgg
       61 atcggtgtcg cccggaggag atagcgtgaa ttcgtattgc agcgtcgcat cgaggaagca
      121 gacacacgaa cccgtcgaat ccaacacgat gggattcgtg ttgggcgtca ggttgtccgg
      181 attgctatag gtgacctgtt tggtcgtggt ccctgcggcg taagtgaaca acaagccgcc
      241 agctagcggc acgccggtgc tcgggttgaa gaactgaagc ttcgggactt gggaggcggc
      301 gacagtcgtc atgcgtcaaa ctccatgttc tcggcatagc ccagtttgtg cagcgtcggc
      361 agcacctttt ccagtcccat gccgatatcg ttgcgaacaa tcatgttggg cacctgcacc
      421 tggccgatga tcttgtcggt cgccttcttc gcggccatga ccgtcttacc cgtgccagca
      481 cagaccatca cgtaatcgcc cgtcgtcttg aagatgtcct tctccacgac cttctcgccc
      541 tccatggccg gacctttgtc caacatcatg ttgacggggt gaatgcctcg ccagtttttc
      601 ggcgtctgca ccggcaggcc catacattgt ccgtcgtcct gctcgtcata cgggaagggc
      661 ggcgtggcaa tcacattgcc gatcgcgacg tcataggaga cgttcagcga atccttgccc
      721 agcaagagat ctcgcttcca ttccgctgga tcttcgggat gggtttcatc cacaatccaa
      781 tcgaacggcc agccgggtcg gcaggtgaac tccaggggcc acggcgtgcc ctccttgtcc
      841 acaatgcaat tgacgtccac atcaccgaga tgccggagct tgcgtagcgc agcaacgagt
      901 ttgggcgtaa agacggcgtc ggccagcttt gattgcttcg tgtactgaat gaccgtgccc
      961 atctcgccgg tgttcgggcc gtagttcccc ggcatcagtt tcttgtgctc gaagttgatg
     1021 ttccacttct cgggcaatgg gccttccggt ccgaaccagc acgacacagc gaactccacg
     1081 ccgtctacgc gctcctgcag catgcacgga cccttgacct tgagtccgtc cttgatccat
     1141 cgctggatgc gccccacgag gtcggcgggc gtcttggcga cgtacgacag gctcttgtct
     1201 tcgcagtcgc ccatggtttt gaacacatag ggctgagtgg ccttccaagc gaacttgagc
     1261 gcggcgtcca gcgagtcgaa catggtgtag ggcggaatgt ccatgccgca cttcttgagc
     1321 acgtccatgc ctttgctgcg gctgatctcc aactgggcgg attcgaagga cggcgcgaac
     1381 acgggaaaac cgtgctgctt ccactggtca agttccttca gaaacttgca gttgctggac
     1441 gtccagatca gtccatcctt ggcccatttc atgctttcgc gccagtcctt caccatggcg
     1501 atgccaggga agccctgccc atccttgaag gcctcgccgt ccacggatcg gttgtaccat
     1561 cgcacgtcgt gacccgcctg cacgcaccgg taggcgaagt cgagtccgca tcggtcggtg
     1621 tcgatcagaa gtacgcgcat cggaggtctt gtgctctatc aagtgttggg atggctaggt
     1681 gcgatggtgt tcgtcgtatg catccgcttg ttccagatcc atctttggac gcacatgaag
     1741 ga
//
LOCUS       DATMTI010000889         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_840779, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000889 DATMTI010000000
VERSION     DATMTI010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840779"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1981)
                     /locus_tag="VN039_14015"
     CDS             complement(143..1981)
                     /locus_tag="VN039_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWV47112.1"
                     /translation="MIDAKPAENLNIVIVGHVDHGKSTLLGRLYADTGSLPDGKLEKV
                     QAICKQQGKEFEYAFLFDAFLEEQEQGITIDTARTFFMWKGRQYIIIDAPGHKEFLKN
                     MISGAARAEAALLLIDALEGVKEQSKKHGYLLSLLGVRQFAVVVNKMDLVGYRQDVFD
                     GIEKEYREFLGQFKAVPSQFVPVSAKLGDNIANRSEQMLWYSGPTVLETLGAFNKEAA
                     RSEQPLRLPVQDVYKFDARRIITGRITAGRLKVGDHLVFSPSNKRANIRTVEAFNIEP
                     QPTEGQAGQSIGVTLDEQIFVERGEIASHQDNLPQVSTAFRANLFWLGKRPLEKGRKY
                     LLRVATKEVDCEVATIHRIIDTMDLAQQQGSSTISKNQVAELTLRTKVPVSFDLSASF
                     EATGRFVLVDEYDIAGGGIITELIHDDQEFLREEARRRDFAWVKGEVTVEDRAQQYGH
                     RAAVVLITGGRHTGKSFLARKLEGRFVADGRHAYLLDGENLRRGLDADLSEEERGQTT
                     EMARRYGEVARLLTDTGLIVVSTTNPFGLAYCEASQAIRTLVHPAPVIAVHMSKSEEE
                     PPPNTDVVLAGPTDFDAATARIVDELKRRGVLAQAIGAKPIFQYSI"
     gene            complement(2173..>2441)
                     /locus_tag="VN039_14020"
     CDS             complement(2173..>2441)
                     /locus_tag="VN039_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HWV47113.1"
                     /translation="PLLDWTEINIWEYIKLENIPFIDLYLDKGNGTRYRSLGCAPCTS
                     PIKSTAKTVDDIIEELRHTTVAERSGRAQDEGRGMELLRKDGYM"
BASE COUNT          489 a          698 c          678 g          576 t
ORIGIN      
        1 gtcgtgcctc gcctctaatc ttggtattac ctgcagctca ttggagctgc ggatcgatgg
       61 ggcattacat taagagtagg ctgccagtga atgaacttag catgtcgaag aaagccctta
      121 gaagttaagt tcgatgaggc agctagatcg aatactggaa gatcggtttc gcaccgatgg
      181 cctgagccag gacaccacgg cgttttaatt cgtcgacgat acgagcggtc gctgcgtcga
      241 aatccgtggg acctgcgagg acgacatcgg tattcggtgg tggctcttct tcggacttgc
      301 tcatgtggac ggcgatgacg ggcgcgggat gcacgagggt cctgatcgct tgtgaggcct
      361 cgcaataggc caggccgaag ggattggtcg tggagacgac gatgagaccg gtgtcggtaa
      421 ggagacgcgc cacttcgcca tagcgccgtg ccatttctgt cgtctgcccc cgctcttctt
      481 cactgagatc ggcatcgagt ccacggcgca gattctcgcc atccaacaag taggcatggc
      541 gcccatccgc cacgaagcgt ccttcgagct ttctggccaa gaacgactta ccggtgtgcc
      601 gtcctccggt aatcagaacg actgccgccc ggtggccgta ctgttgcgcg cggtcttcaa
      661 ccgtgacttc gcccttcacc catgcaaagt cgcgtcgcct ggcttcttca cggagaaatt
      721 cctggtcgtc atggatcagt tctgtgatga tcccgccgcc tgcgatgtcg tattcgtcca
      781 ccaggacgaa gcggccggtt gcctcgaacg atgccgatag gtcaaatgag accgggactt
      841 ttgtgcgcaa ggttagctcg gccacttggt tcttgctgat ggtactgctg ccctgttgct
      901 gggcgagatc catcgtgtcg atgatccgat ggatggttgc cacctcgcag tccacttctt
      961 tggtcgctac acgcaggaga tatttgcgcc ctttttccag cggcctcttg ccgagccaga
     1021 acagattggc ccggaaggcc gtggagacct gcggaagatt gtcctgatgc gaggcgattt
     1081 cgccacgctc gacaaagatt tgttcgtcga gcgtcacccc gatcgattgc cccgcctggc
     1141 cttcggtcgg ttgcggctcg atattgaagg cctcgaccgt cctgatattg gcccgcttat
     1201 tggacggcga gaagacaagg tgatctccca cctttaggcg tccggctgtg atacggccgg
     1261 tgatgattcg gcgtgcatcg aacttataga catcctgcac gggcagtcga aggggctgct
     1321 ccgaccgtgc cgcttctttg ttgaacgcgc cgagtgtttc caagacggtt gggccgctgt
     1381 accacaacat ctgctcgctg cggttggcga tgttgtcgcc gagtttggca ctcaccggga
     1441 caaactgcga cggcacggct ttgaactgtc ccagaaactc ccggtattcc ttctcgatcc
     1501 cgtcaaacac gtcctgccgg taaccgacca aatccatttt gttgacgacc accgcaaact
     1561 gccggacgcc caacagcgac aagagatacc cgtgcttctt cgactgttcc ttgactcctt
     1621 ccaacgcgtc gatgagcagc aatgccgcct cggcacgcgc tgcaccggaa atcatgttct
     1681 tcaggaactc tttgtgcccg ggtgcatcga tgatgatgta ttgccggccc ttccacatga
     1741 aaaacgtgcg agccgtatcg atcgtaatcc cctgctcctg ttcttcaaga aaggcgtcga
     1801 aaaggaaggc atattcgaat tccttcccct gctgcttgca aatagcctgt actttttcca
     1861 acttgccgtc gggcagtgac ccagtgtcgg catagagccg accgagcaag gtggacttgc
     1921 cgtgatccac atggccgacg atgacgatat taaggttttc agcaggctta gcatcgatca
     1981 ttgtcatatt ctttcatccc gcagaatgct caaacgggca gaccagcgag gccgcgggag
     2041 agaaacaacc ggaggcgtac ttcattttcc acccgcccac cccaagctgc caagacagct
     2101 tgttctccgt ggggtacgtt gaggattgtt tcgatccgag aacgaagctg gatgaacgtt
     2161 tcagcgttct gctcacatgt acccatcttt ccgcaataac tccattcctc gaccttcgtc
     2221 ctgtgcccgg cccgatcgtt cagctacggt ggtatgacga agctcttcga tgatgtcatc
     2281 cacggtcttg gcggtggatt tgatcggcga ggtgcagggt gcgcagccta agctgcgata
     2341 gcgcgtgcca ttccccttgt cgagatacaa atcgatgaag ggaatattct cgagcttgat
     2401 gtactcccaa atattgattt cggtccaatc cagcagcgga t
//
LOCUS       DATMTI010000890         4917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_287498, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000890 DATMTI010000000
VERSION     DATMTI010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287498"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VN039_14025"
     CDS             complement(<1..416)
                     /locus_tag="VN039_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47114.1"
                     /translation="MAQQSAHFALYTVNNTSVGISGDGIPGPDTVVSQNSLPDLINLL
                     NQVPALGTKTWVNSGIFGAQCDGVNANVQYVPGVGTLLSVYQPWAGELSSLLSAYVPE
                     TPVPSKPLDDAAVAALIPDTGTATGAAIAEALDAAL"
     gene            complement(425..634)
                     /locus_tag="VN039_14030"
     CDS             complement(425..634)
                     /locus_tag="VN039_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47115.1"
                     /translation="MAWKNKLSSTSSNYDDLQYFYLSSKTGLNTDVDRMLQTFWASLS
                     GLTPAQNYSMADHRAAALAQNPSIP"
     gene            complement(714..1190)
                     /locus_tag="VN039_14035"
     CDS             complement(714..1190)
                     /locus_tag="VN039_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWV47116.1"
                     /translation="MALSDDPRTDPYSFLQQLNQNSANNTQLVTQAAARRRSLMNRAG
                     GRGNGGGGYSGGATSAPSGDIQSYAQQQLAKNGWGNQWDAFNRLVTQESSWNPNAVNK
                     SSGAWGLAQILPSAHRDIRPGSLNANQQIDWMLNYIKGRYGNPNNAWSFHLKNNWY"
     gene            complement(1253..2470)
                     /locus_tag="VN039_14040"
     CDS             complement(1253..2470)
                     /locus_tag="VN039_14040"
                     /inference="COORDINATES: protein motif:HMM:NF033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HWV47117.1"
                     /translation="MAATLASVQAYLKEVYEPRIREQLNSDVKTLKRITRSSSGVSTT
                     IGGKYVTFPVHTRRNSGIGSRLEMEPLPVPGQQGYAAARIGLKYGYGGVQLTGQTIAL
                     SDSDAQAFAKAINEEMDGLKTDIQKDMNRQIYRDGSGAIGVVSTAGTTVNTVTVADAR
                     SFQLNEQVDIVTLPSTVAVSNRTVTAINTATGVVTLSGAAFSSSVGQIVVRNGSGPAA
                     SGNREMTGFDAIIAASGLLYNIDPSTEPEWTANVMSNSGTPRALTEGLMIQMADQINT
                     RGSQASVIFTSKGVRRAYFNLLSQTRQTVNTQDFKGGFTGLAFTTDDGDIPLVSDVDA
                     PLGKLYYVAEDHITYYHDKDWHWADMDGSIWKQVRDSNGDYDAWYARMVEYHELGTDR
                     RNAHGILADITEA"
     gene            complement(2514..3350)
                     /locus_tag="VN039_14045"
     CDS             complement(2514..3350)
                     /locus_tag="VN039_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47118.1"
                     /translation="MSDEGETNAEVTETEAPVETQQESQGNPAWASIQSELDPISFSR
                     IQPKLKEWDDGVQQRLSSVTEKYRWADEIAKSGYQPEQVTMALQAAQMLNEQPEVIYQ
                     KLHEFLEANGRLPQNQQELDDAVEGDEQQDDPRLQALAQQQEQMQQFLIMQENQRLQQ
                     QADAALETEISQLKSSHPEFEDADIQEIISRAALKAQMAASQGVQQIPTLEEAAQDYV
                     SNVRNRILSAKRPGDTAPRLMPTNGGVPASGVAPGTMGGLSKKQAEDLLAELVRQDNA
                     RG"
     gene            complement(3482..3688)
                     /locus_tag="VN039_14050"
     CDS             complement(3482..3688)
                     /locus_tag="VN039_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47119.1"
                     /translation="MADHDVKAATVPALKFTDTRVHPSIAELKAAISGSGVSASYPTA
                     VLQAATKNDLIYICRVHNISVNGL"
     gene            complement(3698..>4917)
                     /locus_tag="VN039_14055"
     CDS             complement(3698..>4917)
                     /locus_tag="VN039_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47120.1"
                     /translation="QAPQQTQLTPLPQYFIDQQDRINTDWEDVTGQHDVSRGSAPSGV
                     TAGTAINFLQEADNQFLTPQHQSIEDGFEKIASQAVALFVQYVDLPRKIKTVGADGTF
                     DTIILSGSDVKNGTDIRMEPGSSVAQSQAAKQAQVTQMFTVGMIDQPTALKLMEMGGV
                     QKVLDVMSVAERKAQRENIKMKLLTPEQIQQHEQAFAIAQLEAQMQQFQMQPQMEPYM
                     PGPAQPQLPGALHPAVPNMDPSGMQISGVGGVPGADPTATGPADGATAPGDPTNGNPS
                     AVMPESPTTAPGVPAGPSPVISVDDFDEHQVHIVTHNKFRMSQEFEALDPAVKQQFAL
                     HVKLHEQALMKGQLQQFFAQIPSDGSDGAGNGSMDVPVGGQGGGAQQPPGATLSANGA
                     VPDPSQSAQSAGN"
BASE COUNT         1071 a         1376 c         1302 g         1168 t
ORIGIN      
        1 tccagcgccg cgtcgagcgc ttcggcgatt gctgctcctg ttgctgttcc ggtgtccggt
       61 atcagagcag caacagcagc atcatcaaga ggcttagatg gcacaggcgt ttcaggtacg
      121 taagccgaca agagagaaga aagctcaccc gcccaaggct gatagacgga tagcagggtt
      181 cctactcccg gaacgtactg cacattagca ttaactccgt cacactgcgc cccgaatata
      241 cccgaattta cccacgtctt agtgcctaaa gcgggaactt ggttaagcag gtttatcaaa
      301 tctggaagag agttttgact tactaccgtg tctggtcctg gaataccgtc gccagaaatc
      361 cctacagacg tgttgtttac agtgtataga gcaaaatgag cactttgctg agccacaata
      421 tctcctatgg gatcgagggg ttctgagcaa gtgctgctgc tcgatgatcc gccatgctgt
      481 agttctgtgc cggagtaagc cccgacaggg aggcccagaa tgtctgaagc atccgatcca
      541 cgtcagtgtt aagtcctgtc ttagagctga ggtagaaata ctggaggtca tcgtagttgg
      601 aagaagtgct actaagttta ttcttccaag ccatgcgtct atgatcctgc aagctgtact
      661 gctcgacagg ggtaagcccg ctggactgtg cccaaaagtc tcgctctagc atatcagtac
      721 caattattct tcaaatggaa agaccaggca ttgttcggat taccgtaacg ccccttgata
      781 tagttaagca tccagtcgat ctgctgatta gcattcaagg aaccggggcg aatatcccgg
      841 tgagctgatg gaagaatctg ggcaagcccc caagctccgc tagacttatt gaccgcgttg
      901 ggattccagc ttgactcttg tgtgacaagc ctgttaaagg catcccactg attgccccag
      961 ccgttcttag caagctgctg ctgtgcatag ctctgaatat cgcctgaagg cgctgaggta
     1021 gctccgcccg agtagccgcc cccgccatta cccctaccgc ccgcacgatt catcagggag
     1081 cgcctacgtg cagcagcctg agtaacaagc tgtgtattat tcgcactgtt ctgattgagc
     1141 tgctgaagaa agctgtacgg gtcagtacgc ggatcgtctg aaagagccat cacccctcct
     1201 aacgtggcag agcccctgtc ggcagtgtat ccgacagggg ttcatcgcct agctacgcct
     1261 cggtgatatc cgcgagaatg ccgtgcgcat tgcgtcggtc ggtacccagc tcgtggtatt
     1321 caaccatacg tgcgtaccag gcatcgtaat caccattgga gtctcgtacc tgcttccaga
     1381 tagagccgtc catgtcggcc caatgccagt ccttatcgtg gtagtaggta atgtgatcct
     1441 ctgccacgta gtacagcttg ccgagaggcg catccacgtc ggacacgagc gggatatcgc
     1501 catcgtccgt cgtgaaggca agccctgtga agccaccctt gaagtcttgc gtattgacag
     1561 tctgccgagt ctgcgagagc agattgaagt aggcccgtcg aacacccttg gacgtgaaga
     1621 tcaccgatgc ctgagaaccc cgagtattga tctggtcggc catctggatc atcaggccct
     1681 cagtgagcgc tcgcggcgta ccgctgttag acatgacgtt ggccgtccac tcgggctccg
     1741 tggagggatc gatgttgtag agcagcccgc tggcagcaat gatcgcgtca aagccggtca
     1801 tttctcggtt accgctagca gccggaccgg aaccattgcg gaccacgatc tgaccgaccg
     1861 agctggagaa tgcagcaccc gaaagagtga caactccagt agccgtgttg atcgccgtaa
     1921 ccgtacggtt cgacacagcg accgttgacg gcagagtgac aatatcaacc tgctcgttga
     1981 gctggaagct gcgtgcatcc gcaacagtaa ccgtgttgac ggttgtaccg gcagtggaaa
     2041 ccactccgat agcacctgag ccgtcacgat aaatctgacg gttcatatcc ttctggatgt
     2101 cagtcttgag gccgtccatt tcctcgttga tcgccttggc gaaagcctgc gcgtcagaat
     2161 ccgacagggc gatggtctga ccggtgagct gcacaccgcc gtatccgtac ttgaggccga
     2221 tacgtgcagc agcgtaaccc tgctggccgg gaaccggcag cggctccatt tccagccggg
     2281 aaccgatacc cgaattgcga cgagtgtgga cagggaaggt gacgtactta ccaccgatag
     2341 tggtagaaac acctgaactg gagcgagtaa tgcgcttgag cgtctttaca tcgctgttga
     2401 gctgttcacg aatcctaggc tcgtatacct ccttgaggta agcctggaca ctagcgagcg
     2461 tggccgccat tttaatctcc tgatcttatt agggtgatcg ggagattcaa cacctaacct
     2521 ctagcgttat cctgacgcac aagttctgcg agcaagtcct cagcctgttt cttgctgagc
     2581 ccgcccatag tccccggtgc tacgccagaa gcgggaaccc cgccgttagt cggcataagc
     2641 ctaggtgccg tatcccccgg tcgttttgct gagagaatcc ggttgcgcac attgctgacg
     2701 taatcctggg cagcctcttc caaagtagga atctgctgaa cgccctggct tgccgccatc
     2761 tgtgccttca aagctgctcg actaatgatt tcctggatgt cagcatcttc aaactcagga
     2821 tggctgctct tgagctgaga aatctcagtt tcaagcgctg cgtctgcctg ctgctgaagt
     2881 cgctgatttt cctgcatgat gaggaattgc tgcatttgtt cctgctgctg agcaagtgct
     2941 tgcaaacgag gatcatcctg ctgctcatcc ccttctaccg catcatcaag ttcttgctga
     3001 ttctgcggga gtcggccatt tgcttcaagg aattcatgga gcttctggta aataacctcg
     3061 ggctgctcat taagcatctg agcggcctga agagccattg ttacctgctc cggctggtat
     3121 ccagacttag caatttcatc tgcccaccga tacttttcag taaccgagga tagacgctgc
     3181 tgaactccgt catcccactc cttgagctta ggctgaatcc tagaaaagga aatcgggtca
     3241 agctcggact gaatactggc ccacgccggg ttaccctggc tttcttgctg agtttctacc
     3301 ggtgcctctg tctcagtgac ttcagcgtta gtttcaccct catcagacat tattcaatcc
     3361 attcttcgcc gtacccctaa aggccctagc gattcttctc tgtgcgccgt accgggacgg
     3421 ccctagcgct tgaggtcagt gtagcgggta ttgccgctca agtgtcaatc gattcggcgt
     3481 gtcaaaggcc attgacactg atgttatgca cgcggcaaat gtagatgagg tcgttcttag
     3541 tcgctgcctg gagtactgcc gtagggtagc tcgcagaaac tcccgaccct gaaatagcag
     3601 ccttcagctc tgcaatgctc gggtgtacgc gagtatccgt gaacttcagc gcgggaacag
     3661 tagccgcctt cacgtcgtga tccgccacga tattctccta gtttccagcg ctctgcgccg
     3721 attgcgatgg gtcggggaca gccccgttag ccgacagggt agcaccgggc ggctgctgag
     3781 cccctccgcc ctgcccacct acaggaacgt ccatagaccc gttaccagct ccatcggagc
     3841 cgtcactggg aatctgcgca aagaactgct gaagttgacc cttcataagt gcctgctcat
     3901 gcagctttac gtgtaaagca aactgctgtt tcacagcggg gtccaaagct tcaaactctt
     3961 ggctcatacg gaacttatta tgcgtaacga tatgcacttg atgttcgtca aagtcatcga
     4021 cagagattac cgggctaggc cctgccggga ctccaggagc agtagtgggg ctttccggca
     4081 tcacggcaga gggattgccg ttagtaggat cacctggagc tgtagcgccg tctgctggcc
     4141 ctgtcgctgt cgggtccgca ccgggaacac cgccgacacc agaaatctgc atgccagacg
     4201 ggtccatgtt cggtactgcg gggtgtagtg caccgggaag ctgcggctgt gcgggaccgg
     4261 gcatgtaggg ctccatctgc ggctgcatct ggaactgctg catctgcgcc tctagctgag
     4321 cgatagcaaa tgcctgctca tgctgctgaa tctgctccgg cgtaagcaac ttcatcttaa
     4381 tgttttcacg ctgcgcctta cgttcggcta cagacattac atcaagtacc ttttgcacac
     4441 cacccatttc cataagcttt agggccgtag gctgatcgat catacctacc gtaaacatct
     4501 gggttacctg agcttgctta gcagcctgac tctgcgcaac ggaagagcct ggctccattc
     4561 tgatatcagt accatttttt acgtctgatc ccgacaggat aatggtgtcg aatgtgccat
     4621 cagcacctac cgttttgatc tttctcggca ggtcaacata ctgcacaaac agcgccacag
     4681 cctggctagc aatcttctca aaaccatcct caatggactg gtgctgaggc gtaaggaatt
     4741 gattatctgc ctcttgcaag aagttaattg cagtacccgc cgtaaccccg gaaggtgccg
     4801 aaccacgaga tacgtcgtgc tggcctgtca catcttccca gtcggtgtta attcggtcct
     4861 gctgatcgat aaaatactgg ggcaaggggg taagctgtgt ttgctgcggt gcctgcg
//
LOCUS       DATMTI010000891         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_330672, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000891 DATMTI010000000
VERSION     DATMTI010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330672"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VN039_14060"
     CDS             <1..890
                     /locus_tag="VN039_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47121.1"
                     /translation="HQGFSTSQILMGGSSIGQATMTRFYSLHFALSLILLVLSELYFY
                     KNRMNRINLKKPVVFLIIGLLLFTALIFPAESGTRSNPEVTPLPILSDWYFLALYQLL
                     KYMDPYWATLWTLGIPFVTIGLTFLDWGNERSWLKRPLFTSIGIMAAIEFVVFSMLII
                     ANKANIETDPPYWFAHLVIMITIGQFWHWSMYKQRLPMTFWVLFNLVTSVWYFIFWHA
                     IVQNTKLNGAFDGHTLTAWVASLQGAAVTINFTNFYWSLLWVGFSVFQLVVALALFWS
                     DRSRRDAREAAMLPEGATA"
     gene            890..1216
                     /locus_tag="VN039_14065"
     CDS             890..1216
                     /locus_tag="VN039_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47122.1"
                     /translation="MALQGDKSLLQKPLSRVMWWVCFLLASFTMSLCFQTFKAFTLVP
                     NWSNGLIWLAAVLLTGQVLLVLVYDSYVQETAKGRIERPVRLLEWMMNKRFLLRAVLE
                     QKEEIK"
     gene            1213..1500
                     /locus_tag="VN039_14070"
     CDS             1213..1500
                     /locus_tag="VN039_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47123.1"
                     /translation="MKRSELTWKDIVAVLLGLSALVGLVIMFNSEAKVVLHNVANPTV
                     VLEQFNAQVLPGIKKYSLAIGAILLAVSISYILLGAQEDISTTLDEKDLKK"
BASE COUNT          336 a          428 c          425 g          429 t
ORIGIN      
        1 cgcaccaagg tttcagcact tcccagattc tgatgggcgg cagttctatc gggcaagcga
       61 cgatgacccg tttctactcg ctgcactttg cgctgtccct gatcctgttg gtgctgtcgg
      121 agctctactt ctacaagaac cggatgaacc gtattaacct gaaaaaaccg gtggttttcc
      181 tgattatcgg gctgcttcta tttacggcgc tcattttccc ggctgaatcc ggtacgcgtt
      241 ccaaccctga ggtgaccccg ctgccgattc tgtccgactg gtacttcctg gctctctatc
      301 agctgctgaa atacatggac ccttactggg caaccctgtg gacgctgggt attcccttcg
      361 taaccatcgg cttgaccttc ctggattggg gtaatgagcg cagttggctg aaacgccccc
      421 tgttcaccag tatcgggatc atggccgcca ttgagtttgt ggtgttctcc atgttgatta
      481 tcgccaacaa ggcgaacatt gagaccgacc ccccttactg gtttgcccac ttggttatca
      541 tgattacgat tggtcagttc tggcactggt cgatgtacaa gcaacgcctc ccgatgacct
      601 tctgggtgtt gttcaacctg gtgaccagcg tttggtactt cattttctgg cacgccatag
      661 tccaaaacac caagctgaat ggcgcattcg atggtcatac cctgaccgcc tgggttgcca
      721 gcttgcaagg cgctgcggtg accatcaact tcaccaactt ctactggagc ttgctctggg
      781 ttggcttctc ggtcttccag ctggtggtgg ccttggccct cttctggtct gaccgcagtc
      841 gccgcgacgc tcgtgaagct gccatgctac cggaaggagc gactgcctaa tggcgttaca
      901 gggtgataaa tcactgttac aaaaaccact cagccgcgtc atgtggtggg tctgcttcct
      961 gctagcctcc ttcactatgt cgctttgctt ccagaccttt aaggcattca ccctggtgcc
     1021 gaactggagc aatgggctga tctggttggc ggcagtgctg ctgaccggcc aggtactact
     1081 ggtgctggtt tatgacagtt atgtccaaga aacggccaag ggacggattg aacggcctgt
     1141 caggctgctg gaatggatga tgaacaagcg attcttgctg agagcagttc tcgaacaaaa
     1201 ggaagagatt aaatgaagcg ttccgagtta acctggaaag atattgtggc agtccttttg
     1261 ggcttgtctg ccttggttgg tctggtcatt atgtttaatt ccgaagccaa ggtggtgctg
     1321 cacaacgtgg ccaacccgac cgtggttttg gagcagttta atgcccaggt tctcccaggc
     1381 atcaagaagt acagtctggc cattggcgct attttgctgg cagtgagtat ttcctacatt
     1441 ctgcttggcg cccaggaaga tatctcgacc actttggacg agaaggattt aaagaaatag
     1501 acgagcgata aggagcccgt acgtgtacac cgaaccgttt caaagagcga cactctggct
     1561 ggagagaggc ctcaacaata tcttctccca gcgctacaac ccgttttatt accatggt
//
LOCUS       DATMTI010000892         3330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_669657, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000892 DATMTI010000000
VERSION     DATMTI010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669657"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..327)
                     /locus_tag="VN039_14075"
     CDS             complement(31..327)
                     /locus_tag="VN039_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWV47124.1"
                     /translation="MIRVVLPAHLRTLARVNGEVTLEVNGPVTQRAVLDALEARYPML
                     RGTVRDHVTRKRRAFVRFFACEQDLSHESPDAPLPDAVVTGSEPFLIVGAMAGG"
     gene            complement(578..1759)
                     /locus_tag="VN039_14080"
     CDS             complement(578..1759)
                     /locus_tag="VN039_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HWV47125.1"
                     /translation="MSGVRLLVGTKKGAFILTSDGTRKRWDVQGPHFGGWELYHLKGS
                     PVDPNRLYASQTSSWFGQVIQCSDDGGKTWNAPGTKPEDLMGSDGMPKGESNMFLYDT
                     SAETGKPLTTHQHYDGSQRPWEFKRVWHLEPSLTDPDTVYAGVEDAALFRSTDGGRTW
                     HELAGLRDVKGNLWQPGAGGMCLHTVMLDRTNPQRIYTAVSAAGAFRTDDGGKTWRAI
                     NRGLKSQYELPDSDADVGHCVHRIAMHPSRPNVLFMQKHWDVLRSDDAGESWHEISGN
                     LPTDFGFPIDVHAHEPNTIYVVPIKSDSEHYPPDGKLRVYRSRTGGTEWEALTKGLPQ
                     ENCYVNILRDAMTVDQLDPCGIYFGTTGGQVYGSANGGDSWEPIVRDLPAVLSVEVQT
                     L"
     gene            complement(1836..2435)
                     /locus_tag="VN039_14085"
     CDS             complement(1836..2435)
                     /locus_tag="VN039_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HWV47126.1"
                     /translation="MRFISLAVTTLCITLTVSPALAKDKKAEKPMDPQAMMELWQKLA
                     APGEPHKQLESLAGSWTTQTKEWMEPGKPPMESGGTVEMKSILGGRFLQQEFTGSMMG
                     QPYSGIGTTGYDNLRKKYVSTWIDSMGTGIFLMEGTASADGKTITLKGGHDEPGGGHM
                     THRAIWKLVDDNTQIFEMYGAHKGEKEMKGMEIVYTRKQ"
     gene            complement(2497..2892)
                     /locus_tag="VN039_14090"
     CDS             complement(2497..2892)
                     /locus_tag="VN039_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWV47127.1"
                     /translation="MLTDASSFPVSKKVLWGGYIMSAIPVVLLLMSGVMKVMVQPTAV
                     EGFAHLGYPESVAFEIGIVEIACLILYLIPQTAVLGAILLTGYLGGAVASHVRIGEPF
                     TGAVVLGVLIWGGLYMRDARLRALIPLRS"
     gene            complement(3019..>3330)
                     /locus_tag="VN039_14095"
     CDS             complement(3019..>3330)
                     /locus_tag="VN039_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWV47128.1"
                     /translation="VMLAGEGLQPSSKGARVRFSGKTRTVIDGPFAETKELIAGYWLW
                     QVKSKEEAIEWVKRCPNPMPGESEIEIRQVFEADDFGAEFTPELRAQEERLRVETTKK
                     K"
BASE COUNT          634 a         1017 c          915 g          764 t
ORIGIN      
        1 aacggtcagc catatgttct tgagtttccg ttatcctccc gccatggccc ccacaatcag
       61 gaacggctcg cttcccgtga caacggcgtc gggcagggga gcatccggcg actcgtgaga
      121 caggtcttgc tcgcaggcga aaaagcgcac gaacgctcgc cgcttgcgtg tgacgtgatc
      181 acggacagtc ccacgtaaca tcggatagcg ggcctcaagg gcgtcgagga ccgcacgttg
      241 cgtcaccgga ccgttgacct caagggtcac ttcaccattg acgcgcgcca gagtccgcaa
      301 atgtgccggg agtacgactc gaatcatttc ttactcgtcg gagttaatgg cgtatagcct
      361 atggcacaag caaaaaagcg atgggcttcg tattcgttcg tgctattagc cataagctat
      421 cagctataca ctcctgactt tagttcatcg gtacggctgc tcaaaatggt cgttcagcga
      481 ggccgcaggg agcgagtcga gtgaggcgta ccctctgggg tacgtcgcag cgatgactgc
      541 gaccgagaac aaagctggcg gccattttca gcagacgtca cagcgtctgg acttcgacgg
      601 acaggaccgc cggcagatct cttacaatcg gctcccaact gtccccgccg ttggctgatc
      661 catagacctg tcctccggtc gttccgaaat agatgccgca gggatcgagc tgatccacgg
      721 tcatcgcgtc ccgcagaata ttgacgtagc aattctcttg cggcagaccc ttcgtcagcg
      781 cctcccactc agtgcccccg gtacggctac ggtacactcg cagtttgccg tcgggcggat
      841 aatgttccga gtcgctcttg atgggaacga catagatcgt gttgggttca tgggcatgca
      901 cgtcgatcgg aaagccgaaa tcggtcggca gattgccgct tatctcgtgc cacgactcgc
      961 cggcatcgtc gctacgcagc acatcccagt gcttttgcat gaacagcacg ttcggccgcg
     1021 acgggtgcat cgcaatgcgg tgtacgcagt gaccgacatc ggcatccgag tcgggcagct
     1081 catattgcga tttcaagccg cggttgatcg cccgccatgt cttgccgccg tcgtcggtcc
     1141 ggaacgcacc ggccgcggag acggcggtat agatgcgctg aggattcgtc cggtccagca
     1201 tgaccgtatg caagcacatc cctccggcgc ccggctgcca cagatttccc ttcacgtcgc
     1261 gtagcccggc cagctcgtgc cacgtccgcc caccgtcggt cgaacggaat aaggccgcgt
     1321 cctcgactcc ggcgtagacc gtatcgggat cagtcagcga cggttcgaga tgccataccc
     1381 gtttgaattc ccacggacgc tgcgagccgt cgtaatgctg gtgcgtcgtg agcggtttcc
     1441 ctgtttcagc ggaagtgtcg tacagaaaca tgttgctttc acccttcggc atgccgtcgg
     1501 accccatgag atcctcgggt ttggtcccgg gggcattcca ggttttcccg ccgtcatccg
     1561 agcattgaat gacttgcccg aaccaactac tggtttgcga ggcatacagc cgattcggat
     1621 ccaccggtga gcccttgagg tggtacagct cccatcctcc gaagtgcggt ccttgtacat
     1681 cccaacgctt acgagtcccg tcggatgtca gaatgaaagc cccctttttg gtgccgacca
     1741 gcagtcgtac cccgctcata taggcctcct ttctcactat ggccggcttt cggcggtcag
     1801 ccgtcagccg cttcgtcagc tcgttgtctg tctgattact gtttcctggt atagacgatt
     1861 tccatgcctt tcatttcttt ttcgcccttg tgagcgccgt acatctcgaa gatctgggta
     1921 ttgtcgtcga cgagtttcca gatcgcgcga tgggtcatat gtcctccacc cggttcgtca
     1981 tgcccgccct tgagcgtgat ggtcttgcca tcggcgctgg ccgtgccttc cataaggaaa
     2041 attcctgtgc ccatcgaatc gatccaggtc gacacatact tcttgcgcaa attgtcatag
     2101 ccggtcgtgc cgatgcccga gtagggttgg cccatcatgc tgccggtgaa ttcctgctgg
     2161 agaaaccgtc cacccagtat cgacttcatt tcaacggtgc cgcccgattc catggggggc
     2221 ttgccgggct ccatccattc tttggtctgg gtcgtccaac tgccggccag gctttccaat
     2281 tgtttgtgcg gttctcccgg cgcggccaac ttttgccata attccatcat ggcctgcgga
     2341 tccatcggct tctcggcctt cttgtctttc gcgagcgccg gcgagacggt gagtgtgatg
     2401 cataacgtcg tcactgccag agatatgaaa cgcatggtga cctccttgtg gttgagtgat
     2461 gggcacaatg aaaaaacact tgctttaccc tgtcggtcag cttcgcaaag ggatgagcgc
     2521 ccgcagtcgg gcatcgcgca tatacagtcc tccccagatc aagacaccga gaacgactgc
     2581 gccggtaaac ggctcaccga tacggacgtg gcttgcgacg gcgccgccca gatagccggt
     2641 gagcaagatt gcccctagca cggctgtctg cggaatgagg tagaggatga gacatgcgat
     2701 ctcgacgatc ccgatttcga acgccacact ttcgggatat ccgagatgcg caaatccttc
     2761 cacggcggtc ggctgcacca tcactttcat cacaccgctc atgagcagca gcactaccgg
     2821 gatggcgctc atgatatagc cgccccagag taccttcttc gagacgggaa aggaactcgc
     2881 atcggtgagc attggtcttc tccttctatc ggtcgtcagg tgacaaaatt ccgtcggtaa
     2941 cgttaccggt atcctgtcgc atcggcggcg gaaaggaagc gaaccccgat tgtccctcgg
     3001 ctttcgagcg agacgtcttt atttcttttt ggtcgtctcc actctcaatc gttcttcttg
     3061 cgctcgcagt tccggcgtga actcggcgcc gaagtcgtcc gcttcgaaga cttgacgaat
     3121 ttcgatctcg gattctcccg gcatggggtt ggggcagcgt ttgacccatt cgatcgcctc
     3181 ttccttcgac ttcacctgcc agagccaata gccggcaata agctctttgg tttcggcaaa
     3241 agggccgtcg atcacggtgc gcgtctttcc cgaaaatctg acgcgcgcac ccttcgagct
     3301 cggctgaagc ccttcacccg cgagcatcac
//
LOCUS       DATMTI010000893         5442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_841024, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000893 DATMTI010000000
VERSION     DATMTI010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841024"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VN039_14100"
     CDS             complement(<1..854)
                     /locus_tag="VN039_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWV47129.1"
                     /translation="MAVGLFVICVAASDEVFAQGAPSSDPSASPRERELRDQLKNILQ
                     ELDELQQKKESEKPEAERPTIIKEKAESASPEAATEAASEANPDFDLADMSIVSKRLQ
                     KRPEGVSLSATVPSETESQPTRTMKESMESLPGVVLRQANGPRDFSIMIRGQGAKTSF
                     AVRDIKIYEDGIQQTQSDGLSRLDMQDPWFMRSVEVTRGASSSLYDNYALGGMVHFRT
                     RRGRDIHGLETFFSGGSFGYQKYGVAIGQETEKFDIAMFGSHVAEDGFIQHSNYNTQT
                     INFNVRYK"
     gene            complement(1003..1308)
                     /locus_tag="VN039_14105"
     CDS             complement(1003..1308)
                     /locus_tag="VN039_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47130.1"
                     /translation="MKRWFAPFVAGMVLLYAALAVSAAGCLMIQTEESEHAHHAPSHA
                     AHSSLCAWICQVNPTVSLHAAAPLLAFFVLVAMQRLVGAAPQLHFISAVSRSRAPPQ"
     gene            complement(1305..1874)
                     /locus_tag="VN039_14110"
     CDS             complement(1305..1874)
                     /locus_tag="VN039_14110"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWV47131.1"
                     /translation="MGSKIRTVPSGLLLLGCMILFGAVDVPHRAATAETDPFAALRVS
                     RIAAGSPIASFDLKELDGRTVRSKELAGKVVLLNFWATWCGPCKEEMPSLARLQSQFD
                     PAQFQVVTVTTDMHPQGIKQFLDHLGIRLPVLFDENEDVSRSFMVRGLPTTVLVTQEG
                     RAVGRAVGPRAWDGEESAALVRHVLEGMK"
     gene            complement(1864..3141)
                     /locus_tag="VN039_14115"
     CDS             complement(1864..3141)
                     /locus_tag="VN039_14115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWV47132.1"
                     /translation="MRNFTATFIVMLSFFVSMHARAEISEPTITLGSKGMTVHQVKNI
                     VGPSVQIDEQGIVSVAWVEEDKEIRTVLFARSEQPGGLLGRPVSVNQPTEHVYYRQES
                     PALAVRGNEIFVAWSQTHPRITSDKPFSGELRLSKSMDGGRSFMPSVLVNDDRQVIQH
                     TFDSIQIAPNGTLHMAWIDGREGKKEPGTFVTRSTDHGRTVAQNLKVDDDTCVCCRTA
                     VATSADGTVYVAWRKIFEGNIRETVVSRSMDDGRTFSSSVVVGNDQWVYPACPHRPAS
                     LGVDQQGRLYVVWYTEGADEMPAIYLVYSDDQGKTFSTKKQLNRSKGTFPDHPQMAVD
                     PAGRIAVIWEEQSPVRREVVVSYSLDRGRSFNAPIKLNEKNGQTPTVAVNQHGTVVMG
                     WKEHAMPAHRLVIQTLQFPMENAMAERTESHGQ"
     gene            complement(3216..4127)
                     /locus_tag="VN039_14120"
     CDS             complement(3216..4127)
                     /locus_tag="VN039_14120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWV47133.1"
                     /translation="MTSTDEIRFFFPAWGISLTLHGVAVGLAFAFAAQVKPVLKENLF
                     QWDVALVEAAKPESISEQAPSAVPPEQPSAKTLSQPRPKQATVVARAVKPLEQKIEPP
                     PPTISPIQTIEQKVENPQPREEPMEQRIVERTESKAEPVVEAKEPEPVTAPPPPHEPV
                     AVAADPASSHEVAAQQEAPAAPLPQEAPVDPAPMEVARAPVAGSETKIDHRWLAESLW
                     RRVAELKRYPNQARMNGQEGKVILKAVIRSDGQLADVFVQKSSGHSVLDAAAIEAVKL
                     ACPLHMKHAIGKPQIVVSLPIVYSLAN"
     gene            complement(4226..4780)
                     /locus_tag="VN039_14125"
     CDS             complement(4226..4780)
                     /locus_tag="VN039_14125"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWV47134.1"
                     /translation="MNSREFKIVFVGILLLAALVASPAFEGWSMGSRVPAVGTAAEDF
                     ALVGLDGKEQRLSQYRGKVVLVNFWATWCKPCTTEMPAMQATYDKLREKGFVVLAINE
                     LEDEAKVREHIKQYGHTFPVLMDRDNKIANQFGVFGLPVSVFIDEKGVVQEYIKGGLL
                     TEQVILDVVARIQKQEPVKAASLH"
     gene            complement(4810..>5442)
                     /locus_tag="VN039_14130"
     CDS             complement(4810..>5442)
                     /locus_tag="VN039_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47135.1"
                     /translation="VYVPSGDHDQTTAGLAGEPGETMEPTAQIGRGQVGLQASFYQTY
                     ELIPHRLNQFATGSYRHTFRNNFGYQWGDQFDFGLGANLVTVPWLVLSNQFNFRYMAR
                     DHFEGSLARSQTPSDPTFPDEAIVLDENIRNRAVPTTGSTFFAYTPGFQINLGDFIQT
                     TLNIKTPLVDNTSLYFYAQVPVYRDFNGNLAQGTSYVVGLTKVFQVLTPS"
BASE COUNT         1141 a         1586 c         1451 g         1264 t
ORIGIN      
        1 atcttgtagc ggacattgaa gttaatcgtt tgcgtattgt agttgctgtg ctggatgaac
       61 ccatcttccg ccacatggct gccgaacatc gcaatatcga atttctctgt ttcttggccg
      121 atggcgacgc cgtatttctg gtacccaaag gatcctcccg agaagaaggt ctccaatccg
      181 tgaatgtctc ttccccgtct ggtgcggaag tgaaccattc cacccaatgc atagttgtcg
      241 tacaaggaag aagatgcgcc gcgagtgact tcgacgctgc gcatgaacca gggatcctgc
      301 atatcgagac gtgaaagtcc gtccgattgt gtttgctgaa ttccatcctc gtagatcttg
      361 atgtctcgaa ccgcgaaaga cgtcttggcg ccctgcccgc ggatcatgat gctgaaatcg
      421 cgaggtccgt tggcttgccg caacacgacg ccgggaagcg attccatcga ttccttcatg
      481 gttctggtcg gctgcgactc tgtttccgaa ggaacggtcg cagaaagtga cacgccttcc
      541 ggtcgttttt ggagacgctt gctgacgatg ctcatgtcgg ccagatcgaa atccgggttt
      601 gcctccgatg cggcttcagt tgcagcctcc ggtgaagccg actcagcttt ctccttaata
      661 atcgtcgggc gctccgcttc gggtttctcg ctctctttct tttgctgcaa ttcatccagt
      721 tcctgaagaa tattcttaag ttgatcgcgc agctccctct cgcgtggtga cgcagatgga
      781 tctgaagaag gcgcaccttg cgcaaacacc tcgtcacttg cagccacgca tatcacaaac
      841 agaccgacag ccatcgcata tctccattgg gaaacacgca ccaattcaaa catgatcgcc
      901 gactcctcgc tcgagcagat tgttgaaggg catagtccga cacacagcct catcagctgc
      961 cgagccggta accgcctacg accgatacga tctagaaaag gatcactgcg ggggagcccg
     1021 agatcgagaa accgccgaaa tgaaatggag ttgtggtgct gcaccaacca accgctgcat
     1081 cgcgaccaag acgaagaacg cgaggagagg cgcggcggcg tggaggctga ccgtcgggtt
     1141 aacctgacaa atccaggcgc agagcgagga atgagcggca tgagatgggg cgtggtgagc
     1201 atgctccgac tcttccgtct gtatcatgag acagcctgct gcgctgactg ccagagcggc
     1261 gtagagcagg accatcccgg cgacgaaggg cgcgaaccaa cgcttcattt cattccttca
     1321 agcacatgtc gcaccagcgc ggccgactcc tcgccgtccc aggcccgcgg cccgacggct
     1381 cgaccgacgg ctcgaccctc ttgcgtaacg agtacggtcg tcggcagacc cctgaccata
     1441 aaggacctcg atacatcttc attctcatcg aaaagcacgg gtagtcggat gcccagatga
     1501 tcgagaaact gcttgatccc ttgtggatgc atatccgtcg tgacggtcac tacctgaaac
     1561 tgtgcgggat cgaattgcga ttgaagtcgg gccaacgacg gcatttcttc tttacacggc
     1621 ccacaccagg tcgcccaaaa attaaggagg acgacctttc cagccaattc ctttgagcga
     1681 accgtccgac catcaagctc cttcaagtcg aacgatgcga tcggagaacc ggccgcaatt
     1741 cgactgactc tgagcgcggc aaagggatcg gtctctgcag tggccgcacg gtgcggaaca
     1801 tcgacggcgc cgaacaggat catacatccg aggagcaata gaccgctcgg cacagtgcgg
     1861 attttactgc ccatggcttt cggtcctttc agccatcgcg ttctccattg gaaattgcag
     1921 ggtttggatc acgaggcgat gcgccggcat cgcatgctcc ttccagccca tcacgaccgt
     1981 accatgctga ttgaccgcca ccgtcggcgt ctgtccgttc ttctcgttca atttgatcgg
     2041 agcgttgaat gaccggccgc gatcgagcga atagctgacc acgacttctc gacggacagg
     2101 cgattgttct tcccagatca cggcaatcct ccctgccgga tcgaccgcca tttgcgggtg
     2161 gtccgggaag gtccctttgg agcggttcag ctgttttttg gtggaaaatg tcttcccctg
     2221 gtcgtcggaa tagaccagat aaattgcggg catctcgtcg gcgccctccg tgtaccaaac
     2281 gacatacagt cgcccttgtt gatcgacgcc gagggatgcc ggacgatgcg ggcaagcggg
     2341 atacacccac tgatcgttgc cgacgacgac tgaagaagaa aacgtccggc cgtcgtccat
     2401 cgatcgagat acgaccgtct ctcgaatatt tccctcgaaa atctttcgcc atgccacata
     2461 cacagttccg tcggcggacg ttgcgacggc tgtcctgcaa cagacgcacg tatcatcgtc
     2521 gaccttgagg ttctgcgcaa ccgtgcggcc atgatccgtc gatctcgtca cgaacgtccc
     2581 cggttctttc ttgccttcgc ggccgtcgat ccaggccatg tgcagggtgc cattcggagc
     2641 gatctgaatc gaatcgaacg tgtgctggat aacttggcga tcatcgttca ccaggacgga
     2701 cggcatgaat gagcgtccgc cgtccatgga cttgctcagc ctcaattcgc cggaaaacgg
     2761 cttatcggac gtgatcctcg ggtgggtctg cgaccacgcg acgaagattt cattcccacg
     2821 gacggctaag gccggggatt cctggcgata atagacatgc tcggtcggtt ggttcacaga
     2881 gacgggtctt ccaagcagcc caccgggttg ttcagatctc gcaaagagga cggtgcggat
     2941 ttccttgtct tcttccaccc atgcaactga gacgatcccc tgctcgtcga tctgcaccga
     3001 ggggccgact atgttcttca cctggtgaac cgtcatacct ttagatccaa gggtgatggt
     3061 cggctcggaa atctcggccc tcgcatgcat cgacacgaaa aatgatagca tcacgatgaa
     3121 tgtagcagtg aagtttcgca tcgcttatcc ctcatgacct tgcatgatct tgaatgagct
     3181 gccttcatga cgtcgtttga ccctcattcg attcactaat tggccaagct atacacgatc
     3241 ggaagactca cgacgatttg aggctttccg atggcatgtt tcatatggag cggacaggcc
     3301 agtttcaccg cctcgatcgc cgcggcatcg agcacgctgt gtcctgaact tttctgaacg
     3361 aatacatccg ccagctgtcc atccgatcgg atgacggctt tcaagatcac tttcccctct
     3421 tggccgttca tgcgggcctg attcggatat cgtttgagtt ccgccacccg tcgccagagc
     3481 gactcggcca accagcgatg atctattttc gtttccgagc cggccaccgg agccctagcc
     3541 acctccatcg gagcaggatc cacgggcgct tcctgtggga ggggtgctgc cggtgcctct
     3601 tgttgggccg cgacctcatg tgatgaggca gggtctgctg caaccgctac cggttcatgc
     3661 ggaggggggg gagccgtaac cggctccggt tccttcgctt ctacgaccgg ctcagccttt
     3721 gattcagttc tctcgacaat acgttgttcc atcggctctt cacgtggctg gggattttcc
     3781 actttctgct cgatcgtttg tatcgggctg atcgtcggtg gaggcggctc gattttctgc
     3841 tccaacggct ttaccgcccg ggctaccact gtcgcttgtt tgggacgtgg ctgcgacaag
     3901 gtcttcgccg atggctgttc cggaggcacg gctgacggcg cttgttccga tatggattcc
     3961 ggcttcgctg cttccaccaa tgccacgtcc cactggaaca ggttctcctt caagacgggt
     4021 ttgacctgtg cggcaaaagc aaatgctaag ccaaccgcta caccatggag ggtcagcgaa
     4081 attccccaag cgggaaaaaa gaagcggatt tcatctgtcg atgtcatatt gtcttctgcc
     4141 tttccatttg ccgctgatcg gttcgcccac tccatgagaa ggaaggctgc tccccccact
     4201 gggcccatac cgacacccta atcgtctagt gcaaagatgc ggccttcacc ggttcttgtt
     4261 tctgaatacg tgcgaccaca tccaggatga cttgctcggt caagagtcca cctttgatat
     4321 attcttggac cacgcctttc tcatcgatga acacgctcac cggcaagccg aacactccga
     4381 actggttcgc gattttgttg tctctatcca taagcactgg aaacgtatgg ccatattgct
     4441 tgatgtgctc gcgcactttg gcctcgtctt ccaattcgtt gatcgccagc accacgaatc
     4501 ccttttcgcg gagtttgtcg tacgtggcct gcatcgccgg catttccgtc gtgcacggtt
     4561 tgcaccaagt ggcccaaaaa ttcacgagca cgactttgcc gcgatattgg ctgagtcgtt
     4621 gctcttttcc atcgaggccg accagggcga aatcttccgc ggccgtacct acggcaggaa
     4681 ctcgcgatcc catgctccac ccctcgaagg cgggcgatgc taccaatgct gcaagtagca
     4741 gaattcctac gaatacgatc ttgaattctc tcgaattcat atgagcctcc ttcaactgaa
     4801 ccgttccgtt taggatgggg tcagtacctg aaacaccttc gtcagaccga ccacatagct
     4861 cgtgccttgc gccagatttc cattgaaatc ccggtagacc gggacttgtg catagaagta
     4921 gagcgatgtg ttgtcgacaa gcggcgtttt gatgttcaac gttgtctgga taaagtcccc
     4981 gagattgatc tggaaacctg gcgtatatgc gaagaacgtt gagccggttg tcggaactgc
     5041 tcggttcctg atgttctcat cgagcacaat cgcctcgtcg ggaaacgtgg ggtcactcgg
     5101 agtctgtgat cgagccagcg atccctcgaa gtgatcgcga gccatgtagc gaaaattgaa
     5161 ctgattgctc agcacgagcc acggcaccgt tacgagattg gccccgagcc caaaatcaaa
     5221 ttgatccccc cattgataac caaagttgtt gcgaaacgta tggcgataac tgccggtggc
     5281 gaactggttc aaccgatggg gaatcagctc ataggtttga tagaacgacg cttgcaggcc
     5341 gacttgaccg cgtccaattt gtgccgtcgg ttccatcgtc tcgcccggct ccccggcgag
     5401 tcctgcagtc gtttgatcat gatcacccga ggggacatac ac
//
LOCUS       DATMTI010000894         4249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_287746, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000894 DATMTI010000000
VERSION     DATMTI010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287746"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..832)
                     /locus_tag="VN039_14135"
     CDS             complement(326..832)
                     /locus_tag="VN039_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV47136.1"
                     /translation="MEGQRDGRSSEISPTILVVDDELPIIKLCKALLEGAGFTVLEAD
                     GSSDALKICTQHQGPIDLLLTDLVLPPQGFQLASIANQFPHVNGHELAVRATMIRSGL
                     RIILMSGNPDKELASHGIKRGTLPFLAKPFERDRLIALVHNVLAQPAPTLAVETRLRA
                     ANDTDWFG"
     gene            complement(943..2385)
                     /locus_tag="VN039_14140"
     CDS             complement(943..2385)
                     /locus_tag="VN039_14140"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HWV47137.1"
                     /translation="MMLTASDVTEVLTEISPLLRGGWIQKIQQPMDQTLMLDVRVPGQ
                     THRLLISCQPDLTRLHITTRSLTNPPTPPPFCQFLRAHFQGARIDDIRQIDNDRIVEL
                     QITGREGAHAIVCELTGSKANILVLDAERRVLRDLTRQFRLAGQAYVPPSQRNTNHKT
                     VPSRFAGKVQGMLPVSEAVDAHYREQEATQTIDRIKEERLRTLKKTFKKEKRLIEAWR
                     NDLTRATAYRDYARYGELIKAHLGAIAKGTDHIEMTDYFDDRLPTITIPLDPMKSAQG
                     NMDDYFRKHQKFLAAERELKPRIERAEHGIERLRQEIQQIEQGTWSPPVISPARLNAA
                     TPVQATVSKGHLATDHRLGPFRRFTSTDGLLILVGRNARENDELTFGLAKSDDLWLHA
                     RGTPGSHVVVRLSKGTDPPPETLRDAAMLALLYSDLKKSGKGDVVYTRRKWVKKAKGQ
                     SPGAVVVTQEKSLHVSLEKNRLEALKSRAS"
     gene            2660..4225
                     /locus_tag="VN039_14145"
     CDS             2660..4225
                     /locus_tag="VN039_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HWV47138.1"
                     /translation="MRLLDLQQLTQLRDEFRLSMRRLFADLCREVHADHADLGRELGI
                     PTDFFDRLHSSLRPGAYSNWKVVGWIETLNDLVYLLDVFRQLKREQDRPEFAEQLLAE
                     CQEKFFEHGYLDDLFPMGRPHARGLEKRLLTLCRRLAQELTFESLWFDPALTLRWSRQ
                     RKVGHWDVFGTLTANCERSEIAGTISVDITGAWCQAPKEVRQALPQASGRMMFRVEPT
                     SITVKAGKMVSPIWSVGGGQGQWQWAYHSPLVAIQRGDDPVTVGPTLVYGKDRQPRTV
                     KPTEQRQVARIARAWQTIRHAWPEGHDVLMLLTSRIVPLQAKGVVSFSYRHRPGLSFI
                     NCFDRGNLDLIDDLIHENSHHHLNLLLRKYVMYHRDHNQQIFYSPWRRSLRPLRGILH
                     ATFTFTMGALLFQRLSSWASGRDGAARWKKAGLTQRDLHRARFRCVEEIESVRYSLQD
                     LHYADHHLGWLTGSGRRLVGLLTEAIEQVERESERFKREVERSAFGQPLLKHVQELQQ
                     ARQTHGPVRLGKV"
BASE COUNT          873 a         1128 c         1244 g         1004 t
ORIGIN      
        1 ccttaccgtt tccggcaaag gtctcaatgt aaccggcggc caatccgagt tcggtatcca
       61 taggtgcgac gctcgctagg aggcagttaa aaaattattt gctgctcgcg cacttttgac
      121 tctaagcgca ggatgctcaa aaaggctgtc caacaaggcc gcagcaagcg aagaggcgga
      181 gacgtacgct tcggtacgtt gagcctctga ggttcacgcc gtgccgaata aggcacggca
      241 cgcttgtgaa cgccgccgag atggtgaggc ggctgtgtct tgcgagaacg ccgctggcgg
      301 actttttcag catcctgcta gacgttcaac cgaaccagtc ggtatcgttg gccgcgcgta
      361 gtctggtttc tactgcaaga gtcggcgcgg gctgtgcgag tacgttgtgc acgagagcaa
      421 tcaagcggtc gcgctcgaac ggtttcgcca aaaaggggag tgtcccccgc ttaatcccat
      481 ggctggcgag ctccttatca ggattccccg acatcaggat gatacggaga ccgctccgga
      541 tcatggttgc gcgaactgcc agctcatgac cgttgacgtg cgggaattgg ttggcgattg
      601 aggcgagctg aaaaccttgt ggaggtaaga ccaggtctgt gagcaataga tcgatcggcc
      661 cttgatgctg cgtgcagatc ttgagagcgt cggaactgcc gtcggcctcg agcacggtga
      721 atcctgctcc ttccagaaga gctttgcaga gcttgatgat cggaagttcg tcgtcgacga
      781 cgaggatggt cggcgaaatc tctgatgatc gaccatcccg ttggccctcc atgtgtgcgc
      841 cctcccgctc tcatcatagt ggtgaacatt tcccagggcg aggaaaacct tcattttggc
      901 aagtgtccca ggaacttgaa aggcggttca cagagaagct atctagctcg cgcgactctt
      961 gagcgcctca agccggttct tctcaagact gacatgcagc gatttttcct gggtaacgac
     1021 aaccgctccc ggcgattgtc ccttcgcctt cttgacccac tttcgtcgcg tgtagactac
     1081 atcgcctttg ccgcttttct tgagatcact gtagaggagt gccaacatgg cggcgtcacg
     1141 gagggtttcc ggtggaggat cggttccttt gctcaatctt acgacgacgt gtgaccctgg
     1201 cgtcccgcga gcgtgcagcc agagatcgtc gctcttagcc agaccgaacg tcagctcatc
     1261 attttctcgc gcattgcgtc cgacaaggat cagcagccca tctgtcgaag taaaccgacg
     1321 gaatgggcca agtcgatgat ccgtggcgag atgaccctta ctgacagtcg cttgaacggg
     1381 agtggctgca ttcaggcggg caggggatat gacaggaggg ctccaggttc cttgctcaat
     1441 ctgctggatc tcctggcgta gccgctctat gccgtgctcg gctcgctcga tacgtggttt
     1501 gagttcacgt tcggcggcaa gaaatttttg atgctttcga aaatagtcgt ccatgttgcc
     1561 ctgggctgat ttcatagggt cgagtggaat ggtgatggtc ggaagccgat catcgaaata
     1621 atccgtcatc tcaatatgat cggtcccctt cgcgatcgcg ccgaggtggg ccttgatcaa
     1681 ttctccatac cgggcatagt ctcgatacgc ggtggctcgt gtcagatcgt tccgccaggc
     1741 ctcaatcagt cgcttctctt ttttgaacgt tttcttgagg gtgcgtagac gttcttcttt
     1801 tatccggtcg atggtttgtg tggcctcctg ttcgcggtaa tgcgcatcga ccgcctctga
     1861 aacagggagc atgccttgga ctttacccgc gaaacggctg ggcaccgttt tgtggttggt
     1921 attgcgttga gatggtggga cataggcctg tccggcgaga cggaattgac gtgtgagatc
     1981 tctcaacact cggcgttcgg catcgaggac caggatattt gccttgctgc cggtcagctc
     2041 acacacgatt gcatgtgctc cctctctccc ggtgatctgc agttcgacga tacggtcatt
     2101 atcaatctga cgaatatcgt caatccgcgc tccctgaaaa tgggctctga gaaattggca
     2161 gaacggtgga ggtgttggag gattggttag tgatcgggtg gtaatgtgca aacgagtgag
     2221 gtccggttgg caggagatca gcagtcgatg ggtctgtccc ggcacgcgca catctaacat
     2281 caacgtttgg tccatgggct gctggatctt ctgaatccag ccaccacgca ggagagggga
     2341 aatttccgtc agtacttctg tcacatctga cgcggtcaac atcatgagtc catcgcaccg
     2401 tcagcccgta ggctcaacct gcctcaattc ggtctcgtga ggataatctg gtctgcggtc
     2461 ttttgtccgc actgtacggc ctactgcaac aagtcgcaag agtaccagga aggtttctga
     2521 catcatcatt acgtgtccta attaatccat gaattgcggc ttggtcacaa cgtcggcata
     2581 gccggtcagg cttctcgctt gcgcctaccc ttccgctacg ctagagttct cccggcattt
     2641 cacgcgtagc cgttccgcca tgcgccttct cgatttgcag cagcttacgc aattacgaga
     2701 tgagtttcgt ctgtcgatgc gacggttgtt tgccgacctg tgccgagaag tgcatgccga
     2761 ccatgctgat ctgggaagag aattggggat cccgaccgac ttctttgacc gtctccactc
     2821 aagcctcagg ccaggagcct attccaactg gaaagtcgtc ggatggatcg agacgctgaa
     2881 tgacctcgtg tatctcctgg atgtgttcag gcagctcaaa agggaacagg atcggcctga
     2941 gtttgcagaa caattgttgg ccgagtgtca ggagaagttt tttgaacatg gctacctgga
     3001 tgacctcttt ccaatgggcc gacctcacgc aaggggatta gaaaaacggt tgttgacgct
     3061 gtgtcgacga ctggcgcagg aattgacctt cgagtcgctc tggtttgatc cggcattgac
     3121 actcaggtgg agtcgtcagc gcaaggtcgg gcattgggat gtgtttggga ccctgactgc
     3181 caattgtgag cgcagtgaaa tcgcgggaac gatctcggtt gatataaccg gagcgtggtg
     3241 ccaggcgcct aaagaggttc gacaggcgtt gcctcaggcc tcagggcgca tgatgtttcg
     3301 agtggaaccg accagcataa cggtgaaggc cggtaagatg gtttccccaa tctggtcggt
     3361 ggggggcggg caggggcaat ggcaatgggc gtaccattca cctctggtcg ctatccagcg
     3421 aggcgatgac cccgtgacgg tggggccgac tcttgtttat gggaaggatc gccaaccgag
     3481 aaccgtgaaa ccgacagagc aacgacaggt tgcacggatc gctcgggcct ggcagaccat
     3541 ccggcacgct tggcctgaag gccatgacgt gctgatgctg ctgacttctc gcatagtccc
     3601 gctgcaggcg aagggggtgg tcagcttcag ctaccggcat cggcctggac tgtcgttcat
     3661 caactgtttc gatcgtggca atctcgattt gattgacgac ctgatccatg aaaacagcca
     3721 tcaccacttg aacctcttat tacgtaaata tgtcatgtac catagggacc ataaccagca
     3781 gatcttctac tcaccctggc ggcggagtct ccggccgttg cgcggcattc ttcacgcgac
     3841 cttcacgttc accatgggtg cgctcttatt tcagcggctt tcgtcctggg cgtcgggacg
     3901 agacggggcg gcaagatgga agaaggccgg tcttacacag cgagacttgc accgggcgcg
     3961 ctttcgttgt gtggaagaaa tcgagtccgt ccgctactcg ctgcaagacc tgcactatgc
     4021 cgatcaccat ctcgggtggc tcaccggttc aggccgccgt ttggtcggac tgctgaccga
     4081 agcgatcgaa caggtcgaac gtgagagtga gcgatttaaa cgagaagtcg aacgttccgc
     4141 cttcggccag cctctgctga agcacgttca agaactccag caagcccgtc aaacccatgg
     4201 accggttcgg ttaggaaagg tgtagcgttg tctggtagag caaagtctg
//
LOCUS       DATMTI010000895         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_161092, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000895 DATMTI010000000
VERSION     DATMTI010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161092"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..2242
                     /locus_tag="VN039_14150"
     CDS             71..2242
                     /locus_tag="VN039_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HWV47139.1"
                     /translation="MKQLFFPLCVATPVPTQSGIRFLHSRTQYDIDNYVDEIWAVLAE
                     CNGYQSIDGIVKKLKQFDDEILRGIIEELTSIGVLIESREIALHFHQFSNNPMLYSYN
                     MSHEDVLEYTGRPRLPIFTGEQFSLSEPSTQLSRLQTARKSTRNFNKEQVSLDELSNV
                     LVSTYAPDSAATPSGGGLYPLKLYMIVTQQGTMPMGYYEYDPLNRSLNLYNDSFDKEV
                     VEFALNSDDLLYGAGAVLVIAADLKRHLGKYSNRGYRYTLLEAGHAAQNVQLACAELD
                     LGCLEYGGFQDEVLSKELQLSEIGVTPLIVLALGHKSSDDYEDSQATLQVLRDELVGP
                     KKPVRYVNITGGDQVAKGDSFFAASALHAPNPQQNARRSYEQRFVGGTGSSSNMAQIK
                     AIAEAYERYASGSLRIDLIASTRTLDSRWLDPRKVAPLTVEQLKAYKKLQAFSESAFY
                     EWVRGVSLANAEPVLLPIDLAFFPVFQEDLGRKPLHAGNSSGVAAYSNLEEAQRRALL
                     EAIERDALMHSWFTRVPPVQISHDRLPLHWRRRAEYWQEQGREVYVLDISRHGVSVTQ
                     VAIVSREAYPSFVNGASASGNSFEESVAKAFHEAELVLLHMLKSPKTTPIDPERVFNP
                     MDHARLYAHPAHLDNLEWVWSGQQTNVIPKVTQSVKSLYQTLDVVSVRLSPENAPLHV
                     VRVLSEKLVPINFGYGSEYYSHPSLEGRVHPDSLKLPHFFA"
BASE COUNT          557 a          718 c          683 g          512 t
ORIGIN      
        1 aaaaaatgat ataatcaccc cataagtcag acctggcttt gcacatttca ctctctggat
       61 tgaggtagca atgaaacagc tattctttcc tctgtgtgta gcgactcctg tgccgactca
      121 gtctggcatt aggtttctac actcgaggac gcagtacgac atcgacaact acgtcgacga
      181 gatttgggct gtccttgcgg aatgcaacgg ctaccagagc atcgacggta tcgtcaaaaa
      241 gctgaagcaa ttcgatgatg agatcctgcg agggatcatt gaagagctca cgagcattgg
      301 agtgctgatc gaaagcaggg agattgctct gcacttccat cagttttcga acaaccccat
      361 gctctactcc tacaacatgt cccacgagga cgtgctggag tacaccggtc gaccacgact
      421 gcccatcttc accggtgagc agttcagcct ctccgagccc agcacccagc tcagcagatt
      481 gcaaacggct cgtaagagca ctcgcaattt caacaaggag caggtgtcac tagacgagct
      541 cagcaacgtg ctcgtcagta cctacgcgcc agacagcgcg gcgactcctt cgggtggagg
      601 tttgtaccca ctaaagctct acatgatcgt cacccagcag ggaacgatgc cgatgggtta
      661 ctacgagtac gaccctctca accggtcact taacctctac aacgactcgt tcgacaaaga
      721 ggtggttgag ttcgcgctca acagtgacga cctgctctac ggggcaggtg cggtactggt
      781 gatcgcggcc gacctgaagc gccacctcgg caagtactcc aaccgcgggt accgctatac
      841 gcttttggaa gcgggtcatg ccgcccaaaa cgtgcagttg gcatgtgccg aactggacct
      901 gggttgccta gagtacgggg ggttccagga cgaggtattg tccaaggagc tccagctcag
      961 tgagataggc gtaacgcccc tcatcgtact cgcactcggc cacaagtcga gtgacgatta
     1021 cgaggactct caggcaacac tacaggtgct tcgcgacgaa ttggtcgggc ccaaaaagcc
     1081 cgtccgctac gtcaacatca ccggtggtga ccaagtggca aagggagatt cgttcttcgc
     1141 tgcgtcggcg cttcatgccc ccaaccccca gcagaacgcg cgccgatcct acgagcagcg
     1201 tttcgtagga gggacgggat cttcgtcgaa tatggcgcag attaaggcga ttgccgaagc
     1261 gtacgagcgc tatgcaagtg gctctctacg catcgacctg atcgcttcca cccgtacgct
     1321 cgactcacgg tggctcgacc ccagaaaggt tgccccactc acggttgagc agttgaaggc
     1381 atacaagaag ctacaggcgt tttccgagtc tgctttctac gagtgggtgc ggggagtgtc
     1441 cctggcaaac gccgaacccg ttcttctgcc gatcgacctc gcattcttcc cggtgtttca
     1501 ggaagacctg gggcgtaagc ctcttcatgc ggggaattcc tcgggcgttg ccgcttactc
     1561 gaaccttgag gaagcacagc gtcgcgcttt gctcgaggca atcgagcgag acgcactgat
     1621 gcacagctgg tttaccagag tgccccccgt tcagatttcg cacgatcgtc tgccgcttca
     1681 ctggcggcgg cgtgcggagt actggcagga gcaagggcgc gaagtatacg tccttgacat
     1741 cagtcgacac ggcgtttcag tgacccaggt cgcgatcgtc tcacgagagg cgtatccttc
     1801 cttcgtcaat ggcgcgtccg cttccggcaa ctcgtttgag gagtcggtgg caaaggcgtt
     1861 ccacgaagcg gagctcgttc tgcttcacat gctgaagtcg cccaagacga ctccgatcga
     1921 cccggagcgt gtgttcaatc ccatggatca cgctcgcctg tatgcccatc cggcccacct
     1981 cgataacctc gagtgggtgt ggtcaggcca acagacgaat gtcatcccca aggtgacaca
     2041 gtcggtcaag tcgctctacc agacgcttga tgtcgtatcg gtacggctct cgcctgaaaa
     2101 cgcaccactc cacgtggttc gcgtactcag cgagaagctg gtgcctatca acttcgggta
     2161 cggttccgag tactactcgc atccgtcact cgaaggcagg gttcaccccg acagcctcaa
     2221 gctgcctcac ttcttcgcgt aatcgcgcgg agataaagta ccaaagaagc aacttaggac
     2281 tatttgcgct cgaaagaggg ttttaggatg ttgaattggg aatgacactg gtgagaatcc
     2341 agagaaagga aggttcggga tgcttctctc gatctcgaag gtcgactacg cgtcgcgcat
     2401 cagcaccggc accagcagca gcaacagctg cagcaacagc tgcgggcaaa gcgcgagctc
     2461 gggtagctcc
//
LOCUS       DATMTI010000896         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_798035, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000896 DATMTI010000000
VERSION     DATMTI010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798035"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VN039_14155"
     CDS             <1..932
                     /locus_tag="VN039_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47140.1"
                     /translation="SSKTSDQQISTKKSSGCEPLYDQQTAESFRESARQQGFELPDRD
                     PIQIVELQNAASKFSARYRTFVSCCVTESTAPAEIVDLIDEANHILKAVQQKGIFNSA
                     GFGVGSGPGGLGGGSAQGPKLGTFARQYTLSEIVGTVAHARQDLVRLKERLEKLSEAQ
                     QGLSDVDYERSGRAKLQIQEDEEAIKKEFKAKRPPSAAPTGMEIQDTTLRSRIGGDIE
                     DTKLNANFGADIGASVSPYSDVSESLRPRRGDDIHDSQLPHRPGTDIQDTSMPSSTGF
                     EVDSAQNREGSSTVPRRGIGASIGDSDLNSKRR"
     gene            complement(940..>1812)
                     /locus_tag="VN039_14160"
     CDS             complement(940..>1812)
                     /locus_tag="VN039_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47141.1"
                     /translation="RDGGQTWTNLSKGMSYSRVIAMAIDPVYPATVYAGTKGDAVYKS
                     HDGGQRWASMRSGLDDATITSVVNQFLFDPADAQHIFLATTMGVFETKTGGEQWTKKM
                     EGMKEVLMVVTLGMDPTRPSILYAGTSGGVYKSIDQAGHWEKVNNGLVPPDMLKTSRA
                     LNVTSILVDPYEPDTVYAATLAGLYKSTDAGKAWKRIGESLADQMIVAMVLDRTRRGV
                     IYVTGRDGVHRSEDAGATWKLVNSGFVTTNIRAIAQSATDPKVFYVGTNGSGLYRSKD
                     AGENWEPMPAVGGK"
BASE COUNT          426 a          544 c          465 g          377 t
ORIGIN      
        1 gctcgagcaa gacatccgac cagcagattt caacgaaaaa gagctcaggt tgtgagcccc
       61 tctacgacca acagacggca gaatcattca gggaaagtgc acgccaacag gggttcgagc
      121 tccccgaccg cgatccgata cagatcgtgg aattgcagaa tgcagcctcc aaattttcag
      181 ctcgctatcg gacgttcgtc tcgtgttgtg taaccgagag caccgcaccg gcagaaatcg
      241 tcgatttgat cgatgaggcc aaccacattc tcaaagcagt tcaacaaaaa ggcattttca
      301 attccgctgg attcggtgtt gggtcgggac caggtggttt gggcggcggc tctgcacaag
      361 gtccgaagct tggcacattc gcccgccaat atacgctcag cgaaattgtc ggaaccgtcg
      421 cccacgctcg tcaagacctg gttcgtctca aggaacgcct ggagaagttg agcgaagcgc
      481 aacaaggtct gagcgacgtc gactacgaaa ggagcggccg agccaaactg cagatccaag
      541 aggatgaaga ggcgatcaag aaagagttca aagccaagag gccgccatca gcagcaccga
      601 ccgggatgga gattcaagac acgacattgc gttcacgaat cggcggtgat atcgaagaca
      661 ccaaactgaa cgccaacttt ggcgcggaca tcggggcctc ggtttctcct tacagcgacg
      721 tgagcgaatc acttcgccca cgcagagggg atgatattca cgacagccaa ctcccgcatc
      781 ggccagggac ggatatacag gacacctcca tgcccagcag cactgggttc gaggtcgaca
      841 gcgcgcaaaa tcgggagggc tcctcgaccg tcccacgccg aggaatcggc gcctccatcg
      901 gcgattccga cttgaacagc aagagacgat agtccattct tactttcctc ccactgcagg
      961 catcggctcc caattctccc ccgcatcctt gctgcgatac aaaccactgc cgttcgtgcc
     1021 gacataaaag accttcggat ccgttgcgct ttgtgcgatt gctctgatgt tggtggtcac
     1081 aaacccgcta ttgactagct tccatgtcgc accggcatct tcgcttcgat gtaccccatc
     1141 ccgccctgtg acatagatta cacctcgacg cgttctgtcg agcaccatgg caacaatcat
     1201 ctgatcggca agcgactccc cgattcgctt ccaagctttc cccgcatccg ttgatttata
     1261 cagccccgca agcgtggccg cgtagacggt atcgggctcg tatggatcga ccaagatcga
     1321 ggtgacgttc agcgcccttg acgttttcag catgtcgggc ggaaccagcc cgttgttcac
     1381 tttttcccaa tggccggcct gatcgatcga cttgtagact ccgccgctgg ttccggcgta
     1441 cagaatggaa ggcctggttg gatccatgcc cagcgtcacg accatcaata cctctttcat
     1501 gccttccatc ttcttcgtcc attgttcgcc gccggttttt gtttcgaata cgcccattgt
     1561 cgtggcaaga aagatgtgct gcgcgtcggc aggatcgaag agaaactgat tcaccacaga
     1621 ggtaattgtc gcatcatcaa gcccggaacg catcgaagcc cagcgctgcc caccatcatg
     1681 actcttgtag acagcatcgc ctttcgtgcc cgcatagacc gtcgccggat aaacaggatc
     1741 aattgccata gcgatcacac gggagtaact cattcctttg gacagattcg tccatgtctg
     1801 cccgccgtca cg
//
LOCUS       DATMTI010000897         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_415896, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000897 DATMTI010000000
VERSION     DATMTI010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415896"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VN039_14165"
     CDS             <1..346
                     /locus_tag="VN039_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47142.1"
                     /translation="VKDIRTSPPADLFEEILELQDTLEEYRASDHGSDQGHRLRATLQ
                     AEQQALERRKEEMESQLRRLFAEWDKLQDAGEATSAARMERDRILKQMRDLLSHRTYI
                     NNIVNDLAATIA"
     gene            370..2190
                     /gene="dnaK"
                     /locus_tag="VN039_14170"
     CDS             370..2190
                     /gene="dnaK"
                     /locus_tag="VN039_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWV47143.1"
                     /translation="MARIVGIDLGTTNSLVAYMKDGQPCVVPDRNGRTMVPSVVAMTD
                     NGLIVGDPAKEHLTRNPERTVYSVKRFMGKGLADVQNELAYFPYTLTETGGVIRIRLG
                     QKSYSPPQISAMILKELKLRVEAHLGESITKAVITVPAYFNDSQRQATKDAGLIAGLE
                     VLRIINEPTAASLAYGLQKNTQGTIAIYDLGGGTFDISILKLKDGIFEVLATNGDTHL
                     GGDDVDRVLVDLFIEEIREREGIDISVYPDHIQTVRLEAERAKIRLSADLKTQITIEL
                     PENKGRFTRELTRDQLESLATNVIERTLAPCRMALKDAGLTPKDIDEVVLVGGSTRMP
                     LVRQRVEALFGKLPHCHLNPDEVVALGAAVQADILSGGTTDMLLLDVTPLSLGIETMG
                     GVMSSLIRRNTTIPASAKELFTTYVDGQTGVDIHILQGERELVKDNRSLARFRLKVPP
                     LPAGVPRIEVTFLIDANGILNVTATDMRTGQSQSIEVKPSYGLSDLEVERMIEDSFKF
                     AAEDIGARKLIEARLDAESLIKTTDKSLAEAGHLVTREETDAIRTALSQLSTAKDGTD
                     PRAIRARMAELEQAAKHLNTVMLDDSLKRGLHGMKVSDVY"
BASE COUNT          560 a          609 c          637 g          442 t
ORIGIN      
        1 ggtcaaagac attagaactt ccccgccggc cgatctcttc gaagagattt tggaattgca
       61 agacactttg gaagaatacc gagcatcgga tcatggttcg gatcagggcc atcggctccg
      121 cgccacgctg caggctgagc agcaggcgtt ggagcgacgt aaggaagaga tggaatctca
      181 acttcggcgg ttgtttgccg agtgggacaa gctacaagac gccggagaag ccacgagcgc
      241 ggcgaggatg gaacgggaca gaatcctgaa gcagatgcgc gatctcctgt cgcatcggac
      301 atatatcaac aatatcgtca acgacctggc ggcaacaatc gcctgagaaa ctcttttcag
      361 cacctagata tggcaagaat agtcggcatc gacctcggca caaccaattc gctggttgca
      421 tacatgaagg acgggcagcc ctgcgtcgtc ccggaccgca atggtcggac gatggtgcct
      481 tcggtcgtcg ccatgacgga caatggcttg atcgtaggag atcctgcgaa agagcatctg
      541 acacgcaatc ctgagcggac ggtctactcc gtaaagcgct tcatgggtaa ggggttggcc
      601 gatgtccaga acgagcttgc atattttcca tacactctca ctgaaaccgg cggtgtgatc
      661 cggattcggc ttggccagaa aagctattca ccgcctcaga tctccgccat gatcctcaaa
      721 gaattgaagc tgcgtgtaga ggcccacctc ggcgaaagca ttacgaaagc cgtcattact
      781 gttccagcct actttaacga cagccagcga caggccacca aagacgccgg cctcattgcc
      841 gggctggaag tcttgaggat catcaatgag ccgaccgccg cctctctggc ctacggactt
      901 cagaagaaca cgcaaggcac catcgccatt tatgacctcg ggggcggcac attcgatata
      961 tcgatcctga aattgaagga cggtatcttt gaggtgctcg cgaccaacgg agatacacat
     1021 ctgggcggag acgatgtcga tcgcgtattg gttgatctct ttatagagga aatccgagag
     1081 cgcgaaggaa tcgacataag cgtctatcct gatcacatac agaccgtccg gctggaagct
     1141 gagcgagcca agatacgcct ttccgctgac ctcaagaccc agatcacaat cgagctaccg
     1201 gagaacaaag gacgctttac cagagagctg acgcgtgatc agctcgaatc cctggcgacg
     1261 aatgtgattg agcgcacgtt ggctccctgt cgtatggcgt taaaagacgc ggggctcacg
     1321 ccgaaagaca tcgacgaagt ggtcttggtg ggcggttcca cccgcatgcc gctggttcgg
     1381 caacgcgtgg aagcactgtt cgggaagctt ccacattgcc acttgaatcc ggacgaagtc
     1441 gtcgcactgg gtgcggctgt gcaagccgat atcctcagcg gcgggacaac agacatgttg
     1501 ttgctggatg tgacgcctct gtctctcggc attgaaacga tgggcggcgt catgagcagc
     1561 ctgatccgtc ggaacacgac cattccagcg agcgccaagg aactgttcac cacctatgtc
     1621 gacggtcaga ccggagtcga cattcatatc ctgcaaggtg agcgcgaact cgtcaaagat
     1681 aaccggagct tggcacgatt ccgcctcaag gtgccgcccc tcccggccgg cgtacctcgc
     1741 atcgaggtga catttttgat cgatgccaac ggaatcttga acgtcacggc gacggacatg
     1801 cggacgggcc aaagccagtc gatcgaagtc aaaccctcct acggattgtc cgatctggaa
     1861 gtggagagga tgatcgagga ttcgttcaag tttgcggcag aggacatcgg tgcgcggaag
     1921 ctgatcgaag ctcgtttaga cgccgaatcc ttgatcaaga cgaccgataa atcattggcg
     1981 gaagcaggac atctggtgac gcgtgaggaa accgatgcaa tccgcaccgc attgtcgcag
     2041 ttgtccaccg caaaggacgg gacggatccc cgtgccatcc gtgcgcgtat ggctgaactc
     2101 gaacaggccg caaagcattt gaacaccgtg atgctggacg attctcttaa gcgaggactc
     2161 catggcatga aagtctcgga cgtatactga agcatatctc gcagataaaa agagcttatg
     2221 gcatatggca cataatcgaa tcgacccc
//
LOCUS       DATMTI010000898         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_330929, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000898 DATMTI010000000
VERSION     DATMTI010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330929"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VN039_14175"
     CDS             <1..396
                     /locus_tag="VN039_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47144.1"
                     /translation="HRPPGSRRDPVSPRRGAATRPEVARRDGMQMRTLIIVARDSMLS
                     DLETLLQSSGVKAYTILNNVLGMGVAGRVFGTFLNPDTNTIIFADLPPDQAENAVSVL
                     QTLHATRKASSHDTPVPLKVFSFPSEEHV"
     gene            complement(496..1929)
                     /locus_tag="VN039_14180"
     CDS             complement(496..1929)
                     /locus_tag="VN039_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV47145.1"
                     /translation="MEGAGNPKTFMRVPPSILVVDDDQRNRDMLAEALSDVGFEVDIA
                     CDGAEALSKANSIPYDAVLSDIRMAPCSGLDLLNSFRKTMPEMPIVLLTAFGSVDTAI
                     QAMKQGAYNYITKPVNLEELVFTMTRAVEHRRLIEDNRTLQRAFSERPRAASLIGQSK
                     KMVEVFKLVGRVSRGRTAVLIQGESGTGKELIARAIHDNSPRAAHRFVAVNCSAIPDS
                     LLESELFGHIKGSFTGAHILRRGLLEEASGGTFFLDEVGDLSLAGQAKLLRVLQEGEI
                     RRIGSNESIRVDLRIIAASRRNLAELVATGRFREDLLYRLNTVNIHIPPLRERPEDIP
                     LLAEFFLARYGDQKEIPVTSFSSAAMRALAGYAWPGNVRELEHVVERAVALASHAILS
                     NDDLPPEVLQSDGSPSDRVDILPGTLKALQREQVLKMLESAQGNKERTARLLGISRRT
                     LYRLLDRYGLGKPRLPTGTDGSDPTGS"
     gene            complement(1896..>3488)
                     /locus_tag="VN039_14185"
     CDS             complement(1896..>3488)
                     /locus_tag="VN039_14185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV47146.1"
                     /translation="GIMAWFIITGVVLVGLSIGIMTLLESDIVNRAGAHNRQVAFLRI
                     AEFMGDMIGKTGGIRNRTVLEELIQDVREVRTGIQRLSVFEITPESSLLILTTDPKAA
                     PQTLDLQERLEIQAGHSVMQLDESSVERAWRITAPIAIDGKVVGALRGLFSVKEYDDL
                     FTQEIELAKTIGIGVVLVTSLTFLLLIRVKIHQPVHRLLYAMRNVEAGDLSGHASITG
                     PVEIQEVTTQFNRMLDRVREAGLEKDRLLEEIRHFNQTLQKRISEATDKLQRANLELV
                     EARLAVEGSQRLAALGELSATMAHELGNPLNALSGHLQMLTNAGDSSSRQRHLAVIRS
                     EVDRMVAIIRQVLDQTRVRLQSAPTNLNSTIQEVLSLLSPGLQKQHVTLKTDLQADLP
                     PVAGDPRALHGLLFNFAANAVQAMPSGGELTIRTRAACGTELPGTVIVSEGALVNGTM
                     VRLTIADTGNGIPLEHLSRIFEPFFTTRHKQGGTGLGLAICHRVVTDSGGRLAVKSAV
                     GQGTEFTVDLPMWKEREIRRRS"
BASE COUNT          717 a         1052 c         1002 g          717 t
ORIGIN      
        1 catcgaccgc cggggagccg gcgggatccg gtctctcccc gacggggggc agccacccga
       61 ccggaagtag cacgaaggga tgggatgcag atgcgaacac tcatcattgt ggcccgcgac
      121 tcgatgttaa gtgacttgga gacgctcttg cagagtagtg gtgtcaaggc ctacaccatt
      181 ctgaacaacg tcctgggtat gggggtagct gggagagtgt ttggcacctt tctcaaccct
      241 gacaccaata caatcatttt tgcggacctt ccgcccgacc aggcggaaaa cgcggtcagc
      301 gtacttcaaa cacttcatgc cacacgaaag gcgtcttcac atgacacccc tgtcccgctg
      361 aaggtatttt cgtttcccag tgaagagcat gtgtagcggc caggtgtagg gtcatggacg
      421 agaaggtgcc ctgtgtcctc atcgcagtct ggaacagtca tgccgcttaa gataaaggat
      481 gtgtgcccat cagggtcagg aaccggtcgg gtccgagcca tcggttccgg tcggcagccg
      541 aggtttgccg aggccgtagc ggtcgaggag ccgatacagc gtgcgccggc tgatgccgag
      601 cagccgcgcc gtccgttcct tgttgccctg cgccgactcc agcattttga gcacctgttc
      661 ccgctgcagc gccttgagcg taccgggtaa gatatcgacg cgatccgacg ggctgccgtc
      721 actctgcagg acttcgggcg gcaggtcgtc gttggagagg atcgcatgcg aagccagcgc
      781 caccgctcgt tccacgacat gctccaactc ccgcacattg cccggccatg catacccggc
      841 caacgcgcgc atggcggccg acgaaaagga ggtgaccgga atttccttct ggtccccgta
      901 ccgcgccaga aagaattcgg ccagcaaggg aatgtcttcg ggtcgctccc gaagcggcgg
      961 gatgtgaatg ttgaccgtat tcagccggta gagcagatcc tcccgaaacc ggccggtcgc
     1021 gaccagttcc gccagattcc ggcgcgatgc ggcgatgatc cgcaaatcca cccggatcga
     1081 ctcattgctc ccgatccgtc ggatttcgcc ttcttggagg acgcgcagca gtttggcctg
     1141 tccggccaac gagagatcgc cgacctcgtc caaaaagaac gtgccgccgc tcgcctcctc
     1201 cagcaagccc cgccgcaaga tatgggcccc ggtgaacgac cccttgatgt ggccgaagag
     1261 ttcgctttcg agcaaggagt cgggaatcgc actgcagttc acggcgacga accggtgcgc
     1321 ggcacgcggg ctgttgtcgt ggatggcgcg agcgatcaat tccttgccgg tgccgctctc
     1381 cccctgaatc aacacggcgg tccggccgcg cgagacgcgt ccgacgagtt tgaagacctc
     1441 caccattttc ttgctctgtc cgatcaatga ggcggcccgc gggcgttcac tgaacgcacg
     1501 ttgaagcgtc cgattgtcct cgatcaggcg tcggtgctcg acggcgcgcg tcatggtgaa
     1561 cacgagttct tcgagattca ccggtttggt gatgtaattg taggctccct gcttcatggc
     1621 ttgaatcgcc gtatcgacgg agccgaacgc ggtcaacagc acgatgggca tctccggcat
     1681 ggttttgcga aacgaattca aaaggtccaa accggaacat ggtgccatac ggatgtcgct
     1741 caggacggca tcatacggga tgctattggc cttactcagg gcttcggcgc catcacaggc
     1801 gatatccacc tcaaagccga cgtcgcttaa ggcttcggcc agcatgtcgc gattccgctg
     1861 atcgtcatcc accacaagta ttgaaggtgg taccctcatg aacgtcttcg gatttcccgc
     1921 tccttccaca taggcagatc caccgtgaac tccgtgccct gtcccacggc actcttcacg
     1981 gcgagccgac cgccgctgtc tgtcaccacg cgatgacaga tcgccaaccc aagtcctgtt
     2041 cccccttgct tgtgtcgtgt cgtgaaaaac ggctcgaata ttcgagataa gtgttccaga
     2101 gggatcccat tgcccgtgtc ggcaatggtc aaacgaacca ttgtgccatt gacgagcgca
     2161 ccttcactca ctatgacggt tccaggaagc tccgtaccgc aagcggcgcg cgtccggatc
     2221 gtcaactccc caccggacgg catcgcctgg acggcgttgg cggcgaagtt gaacagcaga
     2281 ccatgcagcg cacgtggatc gccggcgacc ggtgggagat ctgcttgcaa gtccgtcttc
     2341 aaggttacgt gctgcttttg cagacccggt gaaagcaagg acagcacttc ttggatggtg
     2401 ctgttgagat tcgtgggagc cgactggaga cgcacacggg tctgatccaa cacctgcctg
     2461 atgatggcga ccatgcggtc gacctcggac cgaatgacgg caagatgtcg ctgtcggctc
     2521 gaagaatcgc cggcattggt gagcatttgc aggtggccgg agagcgcgtt taatgggtta
     2581 cccaattcgt gggccatcgt ggcggacaac tctccaagag cagccaagcg ctgactgcct
     2641 tccaccgcga gcctcgcctc gaccagttca agattcgctc gctgcagttt atctgtggct
     2701 tccgagatcc gcttctgcaa tgtctggttg aaatgccgaa tttcctccag gagccgatcc
     2761 ttttccaatc cggcttcccg cacgcgatcg agcatgcgat tgaactgagt cgtcacctct
     2821 tggatttcga cagggcctgt gatcgatgca tggccggaca gatccccggc ctccacattc
     2881 cgcatggcat acaagagtcg gtgaaccggc tgatggatct tgacccggat caacagcaaa
     2941 aaggtcaacg atgtgacgag gacaacgccg atgccgattg ttttggccag ttcaatttcc
     3001 tgcgtgaaga gatcatcata ctccttgacc gaaaacagcc cgcgcaatgc gccgacgacc
     3061 ttgccgtcga tcgcaatcgg ggccgtaatg cgccaagccc gctcgaccga tgattcatcg
     3121 agctgcatga ctgaatgtcc ggcttgaatc tcaagccgtt cctgtagatc caacgtttgg
     3181 ggcgccgcct ttggatcggt ggtcaggatg agcaagctcg attccggcgt gatttcaaag
     3241 actgaaaggc gctgtatccc ggtacggacc tcccgaacat cttggataag ctcttccaga
     3301 accgtacgat ttcggatccc gcccgtcttg ccgatcatgt cgcccataaa ctcagcgatg
     3361 cggagaaacg cgacttgtcg attatgcgcc cctgcccgat tgacgatatc gctttcaagg
     3421 agtgtcataa tgccgatgga cagtccgaca agcacgactc cggtgatgat gaaccaagcc
     3481 atgatacc
//
LOCUS       DATMTI010000899         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_33360, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000899 DATMTI010000000
VERSION     DATMTI010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33360"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..641
                     /locus_tag="VN039_14190"
     CDS             117..641
                     /locus_tag="VN039_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47147.1"
                     /translation="MDILILCLMVLMGWSAWIAFRPSSRHSKANQIPPHTDRASEQIS
                     DLKVDPAELDNIPEPHSRLFTSDDEHQAFTAGQPVWIRYEDKSGAITERVIEIYRPGD
                     DEVIYTWCRRKQAPRTFVRRNIHQWRLLPEHFDFDPIVARYWDEEGIRERVEKNPWRR
                     WLRRQPKDIADRYK"
     gene            complement(1547..2461)
                     /locus_tag="VN039_14195"
     CDS             complement(1547..2461)
                     /locus_tag="VN039_14195"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HWV47148.1"
                     /translation="MNRTAFIISMLGMVFLIAAPVQGGASAASLDTKAIEQAIGKPGE
                     MKDDVYKVSLPRKDLSVSLNGVQLKPGFALGTWIAFKQAGHDAVLDGDLVLTEQEVGA
                     VFGQLRKVGIQVSALHNHLIGETPRVMFLHIEGKGDAGKMAMQVKEALSLTGTPMEPL
                     VASAITIEMSTASEEADFDAEVIQKELGHKGKIKDGVLHVSVPRPESIKMHGTTLPPS
                     MGMATAFNFQSAGGNKVAATGDFVMGRDDVNRVTKALAEHGILVTALHNHLVHGSPDL
                     YFMHFWADNSPEKVAKGLRAGLNAMKRG"
BASE COUNT          908 a         1060 c          977 g          876 t
ORIGIN      
        1 aggcgaaata gatgatcttc agcttattgg ctgatgaggt tgtgagggaa agacccttga
       61 agattgagcc taatgcctcc tgcattcggg cgatgaatgc tcgctggtga gggcgcatgg
      121 acattctcat tctctgtctc atggtgttga tgggttggtc tgcttggatt gcctttcgcc
      181 cctcatcacg ccacagtaag gccaaccaga tcccgcctca caccgatagg gcatcggagc
      241 aaatatccga cctcaaagtc gaccccgctg aactcgacaa cataccggaa cctcacagcc
      301 ggctgtttac ctccgatgat gaacaccagg cgttcacggc tggccaaccg gtctggatcc
      361 ggtacgaaga caaatccggc gcgatcactg agcgggttat cgagatctac cgtccaggag
      421 atgacgaggt gatctacacc tggtgccgga ggaagcaggc cccacgcacc ttcgtgcgtc
      481 gaaacattca ccaatggcgc ctgctgcccg agcacttcga ctttgatccg atcgtcgcac
      541 gatattggga tgaggaaggg attcgcgaga gggtagagaa gaacccgtgg agacgctggc
      601 tgcgtcgaca gcccaaggac atcgctgacc gatacaagta atcaagcgat gtaacagggg
      661 cagcaccaaa aagaaagctc cggcctcttt caagggcgga gcttcatcga acagcgcaat
      721 ttttatattc tgctcttgat agcatttccg ctacgcgtcc aaaaatattg caactgccct
      781 gacgcggctt caaccaggtt cgtgacaccc gtcttctgtt tcggttgaac gaagggatgg
      841 agctgttcag ctatccacgc gtggtgaagg cgctgaccta agaagggcgc ctgtatccac
      901 gaatcttgga ccacttgtcc tctgggaggc ggtacggccg tgatggaggc ttccgtacca
      961 cgagacgctt gataagtagg agcagggctg tgatggaaag agcttggagg agtttcatca
     1021 tcattggcct gattttatga ataaggatac cggctccaag cctgccatgg caacgctatt
     1081 gaccctcaga aaatggtcta gaagactcat gataacccga ccagcgagcc agagggttga
     1141 tgcgcaccca tggagaagag gttcttccat agtatccatt gagatacgac acgtatctgt
     1201 ccacatatca tacatacata agatctggag aactacgtgg acttaccatt ccactcgtag
     1261 caccacccag cgtccgcaca cacaaaaaga aagctccgtc ctctttcaag ggcggagctt
     1321 gtcaaacagc acagcttctt tcattctgtg tcgggtaacg tttccgctac tcgttcaaaa
     1381 tgtccactgc gctgttccag cgtcacctta tcgtcttcag gcccaggccg atactggaag
     1441 aatccctaaa gcgaagcaat ctgaactggg ccgaccaagt tagttacatg cgggacataa
     1501 tcttccatca gccctcgtcg tcccgtctct gccctaaacg atgcactcaa ccacgcttca
     1561 tggcattgag cccagccctc agtcctttgg ccaccttctc gggactattg tcggcccaaa
     1621 aatgcatgaa gtacaaatcc ggcgatccat gtactaaatg gttgtgcaat gccgtgacca
     1681 agatgccatg ttcggcgagc gccttggtga cccgattcac atcatcgcga cccatcacaa
     1741 aatcaccggt cgcggcaact ttgttacctc cagctgattg aaaattgaac gcggtcgcca
     1801 tacccatgct tgggggcagc gttgtcccgt gcattttgat ggactccggt ctcggcaccg
     1861 atacgtgcaa cacgccatct ttgattttcc ctttgtggcc gagttccttt tgaataacct
     1921 cggcgtcaaa atcggcctcc tcactggcag tgctcatctc aattgtgatt gcactggcaa
     1981 ctagaggttc catcggcgta cccgtgagac ttaatgcctc cttgacctgc atggccattt
     2041 tccctgcatc gcctttacct tcaatatgga gaaacatcac ccgaggcgtc tcgccgatca
     2101 gatggttatg gagggcgctc acttgaatgc ctacctttcg gagttggcca aaaacggcac
     2161 cgacctcttg ttctgttaac actagatctc cgtctaagac cgcgtcatgt cctgcctgct
     2221 taaacgcgat ccaggttccg agggcaaagc caggtttcag ttggactcca tttagagaga
     2281 ccgataaatc tttgcgtggc agtgacacct tatagacatc atctttcatc tctccgggtt
     2341 tgccgattgc ttgctcaatc gcttttgtgt cgaggctggc agccgatgct cctccttgaa
     2401 caggtgctgc gatcaagaac accattccca acatgcttat gatgaaggct gtgcggttca
     2461 tagtgtcctc cttcacgtta taatcctatg gatagtctgc catgtcccaa cagttaaggt
     2521 agcggggtac atcactaggc aatgggcctg caggtatcgc ggaaaatcgt tcatgaaatt
     2581 gctctccgcg atggtcgcta tgcccggcca aaacggctaa tcttgaactt atcctgaaag
     2641 ggtggctgta acgggaccac cagacaagga gatcgagcag cgaggcatcg cgttgtttga
     2701 gggtctcttc accgccctgt cacgggtgag tcggccaacc cagatgacgt cactcatgtc
     2761 atcgaaccga tcggcgatcg cctgtgtgcg gtcggattcc tcgtccggct ttcctacagt
     2821 cgatgatgag cttctccttg gcgggattct caaactatgc cgccgcaaca tgcccttcaa
     2881 acgggatgca ccaggaagat ctttcgagag tgcaaagtcc attgatctcg ttggggacac
     2941 cagttcattt acatggccca taacaaggag agatcgctgc gcggcgaggt tgtcctatat
     3001 aggacacgca ctctgtaact cattgtaaaa cctgctactc atcggaccct tctaaggtat
     3061 catcttcttg attttcttca gtttggtctg tcctaaaatg acggcctatt cgatcgacat
     3121 acctcgattc agttaagatg gctacgtgaa cgaaatgctc ggcgaagccc ccgaaggatt
     3181 tactgaagag gtaccgagaa aaagaaaaac ctaaatgaca cggagttttt aagctgcgga
     3241 attgccgccg cgagagcata tggacattct cattctctgt ctcgtagtgt tgatggggtg
     3301 ggctgcgtga atcgcacttc gcccctcatc gcgccggcgc aaggtcaaag cgggcccacc
     3361 tgaccacgat agttcatcgc agaagagagc tggcctcaca ggtgaatata ccgaggtcgt
     3421 cgacatgccg gagccgcaca gccggctgtt gactgccgac gacgaacacc aggcgttcac
     3481 ggctggccaa ccggtctgga tccggtacga agacacatcc ggcgcggtca ctggagcgag
     3541 tcgtcgagat ctaccgtcca cgcgatgacg aggtgatcta cacctggtgc cgtcgaaagc
     3601 aggtcccgcg caccttcgtg cgtcaaaaca ttcaccaatg gcgcctgctg cccgagcact
     3661 tcgactctga tccgatcatc gcacgatgct gggatgagga aggggactcg cgagaggttg
     3721 gaaaaccccc catggagacg ctggttgcgt caacagccca aggaaatcgc cgaccggtac
     3781 aagtaatcta ggatgaacac cccaaagcgc accatcaaaa a
//
LOCUS       DATMTI010000900         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_161169, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000900 DATMTI010000000
VERSION     DATMTI010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161169"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VN039_14200"
     CDS             complement(<1..1362)
                     /locus_tag="VN039_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV47149.1"
                     /translation="MYEAFYHLKTKPFALLPDSSFLYSGSEHQAAYSLLEYGILSQAP
                     FMVLTGEPGVGKTSLLQKLIAEHGNRHKIGLVTHAHYDIEHLLPWILLSLGLSTKRLD
                     PIEAYHMFLAFLAQESKSLRRVILVIDEAQNLGAERLEELRLLSNMNDGKILKLQIIL
                     SGQPDLYTLLQRIDMTQFAQRIVVDYHLKPLTEVDTARYIRHRIRVAGGHPALFTNKA
                     CLLTHRLSQGNPRLINQVSDRALIYGYAEQAKVVTSKLVAQAALDRTKGGILPLAAKD
                     ELIVLATDTEDMTEINDPLPGLETEIHADESFGSRPPDSPEEAYAHGMALKDQGRLKE
                     AVDMFHLAAQGESMRLKAHAQVGLCYRRMREHQAAIQAFRSALNDQSASPNDIIDVLY
                     FLGQSLEFTGQTEEALEVYSRVIQLNPGFANVADRVRGLRQSLEQTSLEKEPGMSNRS
                     WLGG"
BASE COUNT          346 a          421 c          387 g          356 t
ORIGIN      
        1 cccgccaagc catgaacggt tcgacatacc cggctccttt tcgaggcttg tctgttccag
       61 gctctgccgc aaccccctta ctcgatcggc gacatttgcg aaccctgggt tcaactgaat
      121 gacgcgacta tagacttcca gggcttcctc ggtctgcccg gtaaattcca gactttgccc
      181 aaggaaatac aagacatcaa tgatgtcgtt cggtgaggca gactgatcat tcagcgcgct
      241 gcgaaatgct tggatagccg cctgatgctc cctcatcctt ctatagcaga gaccgacctg
      301 agcatgagcc ttcagccgca tcgactcgcc ctgcgccgca agatggaaca tgtctacagc
      361 ctcttttaac cgtccctgat ccttgagagc cattccatgg gcgtacgctt cctctggaga
      421 atccggggga cgagagccaa acgattcatc agcgtgaatt tccgtctcga gaccaggaag
      481 agggtcgttt atctcggtca tatcctcagt atccgtcgcc agcacaatca gttcgtcttt
      541 ggcggccaga ggaaggatcc cccccttcgt tcgatcaagc gccgcttggg ccaccaactt
      601 ggaggtgacg acttttgctt gctcggcata cccatagatc aatgctctgt cggatacttg
      661 attgataagg cgcggattcc cctggctcag acgatgtgtc agcaaacaag ccttatttgt
      721 aaagagggcg ggatgtccac cggcgactcg tattcgatga cgaatatatc gcgcggtgtc
      781 gacctccgtc agtggcttga gatgataatc aacgacaatc cgctgagcga actgagtcat
      841 gtcgatacgc tgcaacagcg tatacagatc aggctgcccg gagaggatga tctgcaattt
      901 taagatcttg ccgtcgttca tgttggagag gagccggagc tcttcgagcc gttcggcgcc
      961 caggttctga gcttcgtcaa tgaccagaat gacccggcga agactcttcg actcctgagc
     1021 caggaatgcc aagaacatgt gataggcctc aatcggatca agccgcttgg tgctcaatcc
     1081 tagggacaac aaaatccaag gtaacaaatg ttcgatatcg taatgcgcat gtgtgaccag
     1141 accaatcttg tgtctattgc cgtgttctgc aatcagcttc tggagaaggg atgtttttcc
     1201 gacaccaggc tcacctgtca ggaccataaa cggcgcctgg ctcaggatgc cgtactcgag
     1261 caagctgtac gcagcctggt gttctgaacc ggagtacaga aaactactat ccggcaaaag
     1321 ggcgaacggc ttggttttca agtgatagaa tgcttcgtac ataggtgcgc actgagtttc
     1381 tttatcgcgc taggtggcta acctcgtctt catctccgcg acagtcaggg ccgatctccc
     1441 ggccttgttc agcactgttc caagaacagg tcgagaaccc ttgattaacg tcaacgcccg
     1501 ctgtagctcc
//
LOCUS       DATMTI010000901         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_117845, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000901 DATMTI010000000
VERSION     DATMTI010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117845"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VN039_14205"
     CDS             complement(<1..1234)
                     /locus_tag="VN039_14205"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV47150.1"
                     /translation="MTGKQVVIIGGGFGGLTAARSLRHADVTLIDRTNHHVFQPLLYQ
                     VATAALSPGDIAWPLRTLFRSQRNVRIIKDDVMSIDRAARMVSLRNSLPIAFDVLIAA
                     PGSRHAYFGHDEWESAAPGLKTMTDAVQIRERMLLAFEEAERRRSSTGVPAQLTFVIV
                     GGGPTGVELAGSLAELGRKAMGPDFPHLRLDDLSVILVEAGPRVLPGFDAELSAKAAA
                     ALTRMGVMIKFRSPVSAVHADGVRIGEEWIPSTNVIWAAGNRASPLLNTLSAPQDSFG
                     RIKVRPDLTIPDDPWIFVIGDAAHCVGRDEKPLPAVAPVAMQQARYVADLIGRDVAPD
                     QRPRFVYADRGMLATIGRAQAMAQFGSIHTSGLFAWLLWCVVHIFFLIGFRNRFRVMS
                     EWIWYYLTFKPGARLIFER"
     gene            complement(1653..2972)
                     /locus_tag="VN039_14210"
     CDS             complement(1653..2972)
                     /locus_tag="VN039_14210"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV47151.1"
                     /translation="MTHKRVVIIGGGFGGMTAARFLRDAEVILIDRTNHHVFQPLLYQ
                     VATAALSPSDIAWPLRTLFRSQSNVRVVMDDVMAINRTARVVQLRDSAPISFDALIVA
                     PGSRHAYFGHDEWEALAPGLKTMADAVHLRERMLLAFEVAERQRAETGAQNRLTFVIV
                     GGGPTGVELAGSLLEIGRKAMGPDYPHLRLEDLSIILVEAGPRILSGFDAKLSAKALT
                     VLERMGVTIKLNHPVTAVRPDGVMVGNEWVPSTNVIWAAGNKASPLLNTLAVPQDSCG
                     RVKVQLDLTIPNEPWIFVIGDAAHCLGRDGKPLPGIAPVAMREGRYVADLINQDRGPD
                     QRPLFTYTDRGMLATIGRAQAVAQIGPVHTSGFLAWALWCIVHIFFLIGLRNRVRVMS
                     EWTWYYLTFKPGARLLCEQPSVQHHMPTKGYVGDTAVENRLSSRRVA"
BASE COUNT          781 a          902 c          927 g          664 t
ORIGIN      
        1 gccgctcaaa aatcaacctc gctcctggtt taaacgtaag gtagtaccag atccattccg
       61 acatgactcg gaagcggttc cggaaaccga tcaaaaagaa aatatggacg acgcaccaaa
      121 gcagccaggc aaagagtcct gaagtatgga tcgagccgaa ctgagccatg gcttgtgctc
      181 ggccgatcgt agccagcatg ccgcggtctg cgtagacaaa tcgaggccgt tggtctggag
      241 cgacgtcccg accgatgagg tcggcaacat accgcgcctg ttgcattgcg acaggcgcca
      301 ctgccggcag cggcttctcg tcgcgcccca cgcaatgtgc cgcgtcaccg ataacgaaga
      361 tccacggatc gtcgggaatc gtcaaatccg gtcggacctt aatacggcca aaggaatctt
      421 gtggagccga gagagtgttg aggaggggag atgctcgatt gcccgccgcc caaatgacgt
      481 ttgtcgaggg aatccattct tctccaatcc tcacaccgtc ggcatggacg gcgctgactg
      541 ggctcctgaa tttgatcatc acgcccatgc gagtaagcgc ggcggctgcc tttgcggaaa
      601 gttcggcatc gaatcccggt agaacgcgag gaccagcctc caccagaatg accgagaggt
      661 catcaaggcg taagtgagga aagtccggtc ccatggcttt tctgccgagc tcggccagcg
      721 aaccggctaa ttcaaccccc gtcggccctc ctccgacaat gacaaacgtc agctgggccg
      781 ggacaccggt cgagctcctt cgtcgttccg cctcctcaaa tgcgagcaac atcctctctc
      841 gaatctggac ggcatcggtc atcgtcttga gtccaggcgc agccgactcc cattcgtcgt
      901 gtccgaaata ggcatggcgc gagcctgggg cagcaatcag gacatcgaag gcaatcggca
      961 aactattccg caggctgacc atccgagccg cccgatcgat cgacatgacg tcatccttga
     1021 taatgcggac gttgcgctgc gaacgaaaga gtgtgcgtag tggccaggca atatcaccgg
     1081 gcgacaatgc ggcggtggct acttgataga gcagaggttg aaagacgtgg tgattcgtgc
     1141 ggtctatgag ggtaacgtcg gcatggcgta gagatcgcgc agccgttaaa cctccgaacc
     1201 cgcccccaat aatgacaact tgcttgccgg tcattctacc gtgcccggtg aaagtatgat
     1261 cccgtcggtt tgttgtggtg tcggtctgtc ttccgatcgc cgtcggctca gggtgtagta
     1321 tcccagagcc tattgcgggg tcggacttcg aatagagttc acttcatgtt tttccgactc
     1381 tatgccagtg agagagcgat gaatgggcat tgtaatgttg gctgtcgcat ctcacgccga
     1441 caagctcctc cgcttgtcac catgctttgc actttatcgt gcctgtccat gatagtgaag
     1501 tcccaagagg gtcacaattt aactgtattg ggctgggatc ggagacaacg ttaccgaaag
     1561 gatacgggag ccacttccaa cgaataattg aagactgaga ataattgagg gctgaataga
     1621 cgaagggtga gctggtttag ggatttctga tattacgcca cacgacgtga cgacaggcga
     1681 ttctcaacag cggtgtcgcc tacgtatcct ttggtcggca tatggtgctg aacagaaggt
     1741 tgttcacaga gcaatctcgc acccggcttg aaggtgaggt agtaccacgt ccattccgac
     1801 atcacacgta cgcgattcct gagaccgata aggaagaaaa tgtgaacaat acaccaaagt
     1861 gcccaggcca agaagccgga cgtgtggacc gggccgatct gagcgacagc ctgagcgcgg
     1921 ccaatcgtgg ccagcatgcc acggtctgta taggtaaaca atggacgctg gtctggacct
     1981 cggtcctgat tgatcagatc ggcaacatac cgtccttctc gcatggcaac cggagcgatg
     2041 cctggtaagg gtttgccatc gcggccaaga caatgggcag cgtcaccgat gacgaagatc
     2101 cagggctcat tggggatcgt caggtcgagt tggaccttaa cccggccgca cgaatcctga
     2161 ggaactgcca aggtgttgag caagggcgac gccttgttgc ccgccgccca gatcacattg
     2221 gtagagggaa cccattcgtt gccgaccatg accccatcgg gacggactgc ggttaccgga
     2281 tgattaagtt tgattgtcac ccccatacgt tcgagcaccg tcagggcctt tgccgacagc
     2341 ttggcatcaa atccagagag aatacgcggg ccagcctcga ccaggatgat cgaaagatcc
     2401 tccaagcgca agtgaggata atccggcccc atggcttttc tcccgatttc gagaagcgaa
     2461 ccggctaact cgactcccgt ggggcctcct ccaacgatga caaaggtgag gcgattctgc
     2521 gcaccagtct cggcccgttg ccgttctgcc acctcaaatg cgagcaacat ccgctcgcgc
     2581 aaatggacgg catccgccat ggtcttgagt cccggagcca atgcttccca ctcatcatgt
     2641 ccaaagtaag cgtgtcgtga gcctggagcg acaatcaagg catcaaagga aatgggagcg
     2701 ctgtcccgaa gttggaccac ccgagcggtc cgattgatcg ccatcacgtc atccatcact
     2761 acacgaacat tggactgtga acggaagagt gtacgtaacg gccaggcaat gtcgctcggg
     2821 gacaatgcgg cggtggccac ctggtagagc aagggttgaa agacatggtg attcgtgcgg
     2881 tcgatcagga tgacctcggc atcgcgcaga aagcgcgccg ctgtcatacc tccaaacccg
     2941 cccccaatga tgacaacgcg tttgtgagtc atgggcttga atcaggaggc acggctactt
     3001 acgaaagcga ataggcttaa ccgctggaat gaaatgtccg cggtggccgg ctacacagcc
     3061 tgctaggatc atcgtgatca aggccaatag aagcagttcc acgtctcacc tcctgaaaat
     3121 tgttcgatgc aataagtggt taaggaagga tatgttcgaa aacaagctac gtgctaaggc
     3181 aaagagtgtc aaagatttcc tacaaacccg cttgagcact cttctctaag aatcggcagg
     3241 cccgggtgtt tccttgagtc cattggcgac gatg
//
LOCUS       DATMTI010000902         5343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_75567, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000902 DATMTI010000000
VERSION     DATMTI010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75567"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /gene="kdpC"
                     /locus_tag="VN039_14215"
     CDS             <1..493
                     /gene="kdpC"
                     /locus_tag="VN039_14215"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HWV47152.1"
                     /translation="GLAQVFFSDQANGSLMVREDKVIGSKLIGQYFDKPEYFWGRPSA
                     TAQFPYNAAASGGSNLGPTNPVLIETVKARVAVLRTADPSNDSLIPVDLVTASGSGLD
                     PHISPAAALYQVKRVACARGLDQTIVQKLVVQHTEERQFGLLGERRVNVLQLNLALDS
                     LLR"
     gene            complement(774..974)
                     /locus_tag="VN039_14220"
     CDS             complement(774..974)
                     /locus_tag="VN039_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47153.1"
                     /translation="MGNMDIGTLIIAGLCGIGLIQFIMWIVVMIRHPGCDDLRLDLVK
                     AASPRSSETKAPPQEADDDRSS"
     gene            1136..3838
                     /locus_tag="VN039_14225"
     CDS             1136..3838
                     /locus_tag="VN039_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWV47154.1"
                     /translation="MHSQRPDPDVLLKRVQAEEARRVEGKLKIFFGANPGVGKTYAML
                     ETAHEQRRDGVDVVIGVVETHGRVETQALVNGLEVVPRRAVEHRGTLLQEFDLDAALV
                     RRPAIILIDELAHSNAPGLRHAKRWQDAQELLKAGITVYTTVNVQHLEGLNDVVAQIT
                     GVRVRETIPDSVLERADDVELIDLPPDDLLQRLKDGKVYVPEQIQHAIHNFFTKGNLI
                     ALRELALRRTAERVDQQMEVYRRDHAVVRIWPAAETIMVCINMKSRGPRLIRAARQMA
                     ADLHAKWIAVYVQIPRHLRLPQTERDRLVQTLRLAEQLGAETVMLTGENVAQEILSYA
                     RSRNATKILVGKPVRPVWKEWIFGSVVSDLVHRSGEIDIYVIAGEAGEGQPLIRRSLR
                     STSDVRAYGYASLGVLGATSVAWLMFPYFAAANLIMVYLIAVIVIAIRWGRGPSVLAS
                     ILSVAAFDFFFVPPYFSFAVTDIEYLLTFGVMLVVALIIGNLAVRIRQQAELARDREH
                     RTGVLYSMSRDLATHRGTGMLAQLAAKHLRDVFDAQIAVFLADADKKVQLKRGELLFF
                     ELEPKESGVAQWVYDHNERAGLGTDTLPGASALYVPLVCSTGPIGVVALRPKNAALLL
                     DPEQLHLLESLMSQVALAIERACLSDEAQQAHVRAETERMRNAILSSVSHDLRTPLAT
                     ITGAASSLAAERGEFAPVARRELAQSIYREAERLDRLLKNLLDMMRIEAGVVQLKKEW
                     HSVDEVAGAALAGLDGRLHDHAVNTSFPADLPLVLVDGVLLEQVVINLIENAVKYAPA
                     RTTIELAASATDREIVVEVADRGPGIPVGEESRIFDKFYRAKPAREGGVGLGLTICRG
                     IVEAHGGRIWAENRSGGGALFRFSIPLPDWQPPVETEQSEAAET"
     gene            3866..4558
                     /locus_tag="VN039_14230"
     CDS             3866..4558
                     /locus_tag="VN039_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV47155.1"
                     /translation="MSQDATILLIEDEPEIRRFLRTTLPAHGFRLHEAATGQDGLTEA
                     KARNPDLILLDLGLPDLDGGEVIRRVREWTTTPIIVLSARDQEQAKVAALDLGADDYV
                     TKPFGVNELLARVRTALRHAARTGDGDESVFVLDDLKVDLGRRQVFVSGKEVHLTPIE
                     YKLLTTLIRYAGRVLTHRQLLKEVWGPLHVDEGHYLRVYMRQLRNKLERNPAHPRYLV
                     TELGVGYRLRTE"
     gene            4688..>5343
                     /locus_tag="VN039_14235"
     CDS             4688..>5343
                     /locus_tag="VN039_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWV47156.1"
                     /translation="MQAGAGRIFPVAVKTIGQQDQRCKHISRLLIACGVLATVPVCQA
                     QDLSQQSKENPSLSGASNPVKTETTNWHYGAYVDVSYIGNFNFPDNHLWRSRATANHD
                     NELSPNMGLAYVRKGASESSPWGMELGFQGGRDTEEFAFLVGEKRLDGSDVLRHVHRA
                     NVSYLAPIGKGLTVTAGLFNSLMGYESLYARDNANYTRSWIADNTPYMMFGVNAQYPV
                     "
BASE COUNT         1133 a         1417 c         1591 g         1202 t
ORIGIN      
        1 agggctcgcc caagtgtttt tctcagacca ggcgaacggc agtttgatgg tgcgtgaaga
       61 caaggtgatc gggtccaagt tgatcggaca gtatttcgat aaaccagagt atttctgggg
      121 ccgtccatcg gcgactgcgc agtttcctta caatgccgct gcgtcgggtg gatcgaacct
      181 cggacctact aacccggtac tgattgaaac agtcaaggcg agagtggctg tcttgcgaac
      241 ggcagatccg agtaacgact cgctcatacc ggtcgacctg gtcaccgctt cgggaagtgg
      301 gctggatccc catatcagtc cggcggccgc cttgtatcaa gtgaaacgag tagcctgtgc
      361 gcgaggtctg gaccagacca tcgtacaaaa gctggtggtt cagcacacag aagaacgcca
      421 atttgggtta ctgggagaac ggagagtcaa tgtcctgcaa ttgaatctgg cgctcgattc
      481 gttgcttcgc tgagccaatt ccccgttgaa acgcccgata ccgcaggagg tgtttggtgt
      541 cactgctcat cctatttctg gttacacttt gggcgatggc gttcttgatc tggctgttgt
      601 ggaaatggct acctccgggc ggcctggtgc accgtccgcc aagagattac atcagcaagc
      661 cgtaagcatc tccgatcggt cgacacgact tgactgaatt tattccgatc gttgggggtt
      721 cattgccaat ttctcgttgc ttcacaagcc caccggtttg gatatggctt gggtcatgaa
      781 gatctgtcgt cgtcagcctc ttgtggtggc gctttcgtct cgctggaacg aggagaagcg
      841 gccttgacca ggtcaagtcg cagatcatca catccgggat gtcgaatcat cacgacaatc
      901 cacatgataa actggatcag tccaataccg catagccccg caataatcag cgtcccgata
      961 tccatgtttc ccattgtggc tctttccttt gttatagaaa acagcatagt cccacgacat
     1021 caggatgtgg taagaaggca gccttaggct atcaatatgg cgtaaaattc gtattgtccg
     1081 gccttttggt gtcggccgca gccgccaacg ttgtggacat gaactgagat ccgcgatgca
     1141 ttcacaacga ccagaccccg atgtcttgct gaagcgtgta caagccgaag aagcccgtcg
     1201 tgtggaaggt aaactcaaga tctttttcgg agccaatccc ggcgtaggga agacctatgc
     1261 aatgttggaa accgcgcacg agcagcggcg tgacggcgtc gatgtcgtca tcggcgtcgt
     1321 ggagacgcat ggtcgcgtgg aaacccaggc tctagtgaac gggctggaag tggtcccgcg
     1381 ccgcgcggtc gagcatcgcg gcaccctgct gcaggaattc gacctcgatg cggcacttgt
     1441 gcgccgtccc gccatcatcc tcatcgacga actcgcacac agcaacgcgc cgggcctgcg
     1501 tcatgccaaa cggtggcagg atgctcagga attacttaag gccggcatca cggtctacac
     1561 caccgtgaac gtgcagcatc tcgaaggtct gaacgatgtg gttgcccaga ttaccggggt
     1621 tcgggtgcgt gagactattc cggattccgt actcgaacgg gcagacgatg tggagctgat
     1681 cgatcttccg cctgatgacc tgcttcagcg gctcaaagac gggaaggtgt atgtcccgga
     1741 gcaaatccag catgcgattc acaatttctt caccaaaggc aatctgatcg cgcttcgcga
     1801 acttgctctc cgccgtacgg ccgagcgggt cgatcagcag atggaggtct accggcgcga
     1861 ccatgcggtc gttcgaatct ggcctgcggc ggaaaccatc atggtctgca tcaacatgaa
     1921 gtcacgcggc ccgcgcctga tcagagccgc ccgccagatg gctgccgatc ttcatgccaa
     1981 atggatcgca gtgtacgtgc agatccctcg gcatcttcgc ctaccacaaa cggaacgaga
     2041 tcgcctggta caaaccctac ggttggcgga gcagcttgga gccgaaacgg tcatgctaac
     2101 cggcgaaaac gtcgctcaag agattttgag ctatgcgcgg agtcgcaacg ccacaaagat
     2161 tcttgtcggg aaaccggtac gcccggtgtg gaaggagtgg atctttggct ccgtggtgtc
     2221 cgaccttgtt caccggagcg gcgagatcga catctatgtc attgccggag aagcgggcga
     2281 aggtcagcca cttatccgcc gtagtctccg aagcaccagc gatgtccggg cttatggcta
     2341 tgcctcattg ggagtactgg gtgcgacgtc ggtcgcctgg ctgatgtttc catatttcgc
     2401 ggcggcgaat ttgatcatgg tgtatctcat tgccgtgatc gtcattgcga ttcgttgggg
     2461 gcgtgggccg tcggtgctgg cttcgatttt gagcgtggct gcatttgact tttttttcgt
     2521 gcctccctac ttctccttcg ccgtcaccga catcgaatat ctgttgacat tcggcgtgat
     2581 gcttgtggtc gcactgatca tcggcaatct agcggtacga attcggcaac aagctgaact
     2641 ggcgcgtgat agagagcatc gcacgggtgt gttgtattcc atgagccgcg atctcgccac
     2701 tcatcgagga acggggatgt tggcccaact cgcagccaag catcttcggg atgtgtttga
     2761 cgcgcagatt gccgtattcc tcgcggatgc cgataaaaag gtacagctga aacgggggga
     2821 actcctgttt tttgaattgg agcctaagga gtcgggtgtc gctcaatggg tgtatgacca
     2881 caatgagcgg gccggcctcg ggaccgatac gcttccgggg gccagcgcgc tgtatgtgcc
     2941 gttggtatgc tcaacggggc cgatcggcgt cgtcgctctc cggccgaaga acgccgcgct
     3001 tctgctggat cctgaacaac tgcatttgct ggaatcgctg atgagccaag ttgccttggc
     3061 gatcgaacga gcttgtttat ccgacgaagc tcagcaggcg catgtacgag cggaaaccga
     3121 gcgtatgcgt aacgccatac tgagttcggt ctcccacgac ctgcggacgc cgctggccac
     3181 gatcacgggc gccgccagta gtttggcggc ggaacggggg gaattcgctc ctgttgctcg
     3241 gcgcgagctt gctcaatcca tctatcggga agcggagcgc cttgaccgtt tgctgaagaa
     3301 ccttctcgat atgatgcgca ttgaagcagg ggttgtgcag ctcaagaagg aatggcattc
     3361 ggtggatgaa gtggcgggag cggctttggc cggacttgat ggacgattac acgatcatgc
     3421 cgtcaatacg tcgtttcctg cggatcttcc gctggtgctt gtcgatggag tgctcctgga
     3481 acaagtcgtc atcaatctta tcgagaacgc cgtgaagtat gcgcctgcga gaacgacgat
     3541 cgagctggct gcgtcggcga ctgatcgaga gatcgtcgtg gaagtcgcag atcgaggacc
     3601 gggcatcccc gttggggagg aatcccgcat cttcgacaaa ttctatcgtg caaagcctgc
     3661 gcgagaggga ggagtcggcc tgggactgac gatttgtcgt ggtattgtag aagcgcatgg
     3721 cggccgtatc tgggctgaga atcgctccgg aggcggagct ctctttcgct tctcgatccc
     3781 gttgcctgat tggcagccgc ctgtagaaac cgagcagtcg gaggctgcgg aaacgtaatt
     3841 ctgatcaagc gagccggcag atcctatgtc acaggacgcc accatactcc tcatcgaaga
     3901 tgaacctgag atccgtcggt tcctgcggac gacacttccc gcgcatggct ttcgcctcca
     3961 tgaagccgcg acgggacagg atgggcttac agaggcgaaa gcgaggaatc ccgatctcat
     4021 ccttctggac cttggtctgc ctgatctcga cggaggcgag gtcatccgcc gggtgcggga
     4081 gtggactaca acacctatca tcgtactgtc tgctcgtgat caggaacagg cgaaggtggc
     4141 ggcactcgat ctcggcgccg acgactatgt caccaagccg ttcggagtga acgaacttct
     4201 cgcacgggtg cgcaccgcgc ttcgtcatgc tgctcgaacc ggtgatggtg atgaatcggt
     4261 gtttgtgctc gacgatctga aggtcgatct cgggcggcgg caggtgtttg tctcagggaa
     4321 ggaagttcat ctcacaccga tcgaatacaa gctgctcacc acgttgatcc gctatgccgg
     4381 cagagtgttg acccaccgcc agctgttgaa agaagtctgg gggccgcttc atgtggatga
     4441 agggcactac ctacgggtct atatgcgtca attgagaaac aaactggaac gcaatccggc
     4501 gcacccgcgt tatctggtga cggagctggg ggtcggatat cgacttcgga cggaataacc
     4561 tcactgccct tcttcacgct ttgctgacat ccgcctcatc tgtttttatc ccgttttgat
     4621 acctgacgaa gtatagtttg tgggtcaaat gtaggagttt ccacccgttc ggttgaaggc
     4681 gcatacgatg caagcgggcg ctggtcggat attcccggta gctgtgaaga cgattggtca
     4741 acaagatcaa cggtgcaaac atatcagccg gttgctgatc gcatgtggag tactcgcaac
     4801 cgtgcctgtc tgccaggccc aggacttgag tcagcagtcg aaggaaaacc catctctatc
     4861 cggggcatcc aatcccgtga agacggagac taccaactgg cactacgggg cttatgtgga
     4921 cgtgagttac atcggtaatt tcaattttcc cgacaaccat ctctggcgca gtcgcgccac
     4981 ggcaaaccac gataatgagc tttcgcccaa catggggctg gcgtatgtgc gaaagggcgc
     5041 gagtgagtcc tcaccgtggg ggatggagct cggcttccaa ggcggccggg atacagaaga
     5101 gttcgctttt ttggtgggtg aaaaacgact cgacggatcg gatgtgctga ggcacgttca
     5161 tcgtgcgaac gtgtcatatc tggctccgat tggaaaagga ctgacggtca cggcaggtct
     5221 attcaacagc ttgatggggt atgaatcact ctatgcgaga gacaacgcca actatacgag
     5281 atcgtggatt gcggacaata cgccttacat gatgtttggc gtgaatgcac agtatcctgt
     5341 gag
//
LOCUS       DATMTI010000903         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_669977, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000903 DATMTI010000000
VERSION     DATMTI010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669977"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..460)
                     /locus_tag="VN039_14240"
     CDS             complement(38..460)
                     /locus_tag="VN039_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HWV47157.1"
                     /translation="MAVEAGYYIYGTPAGGAYQYAHPAMMSVLFWVEREWATTESRQF
                     GIGNISMANGESTGEHHSHKDGLQVDVRPLRKDGAHLPVTWMQSEYDGEATAKLIAIF
                     FSHPLVKQVLFNDNRIPGVRHWVRHDDHFHVGIKLGAK"
     gene            complement(450..1232)
                     /locus_tag="VN039_14245"
     CDS             complement(450..1232)
                     /locus_tag="VN039_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47158.1"
                     /translation="MIVLPDPTWDSVAVEAIAEKLDKRKRHSWRLQSGKIFFKHRRAE
                     YHLKAARQTVREMVDAMQPAPVTASVEDPVNSSYFGPLTEPLFFHLDGFLEATRSAHD
                     AVMDFLISAEVVPHDSPHSINAFVKRINNTPDRSRTNPVVVAGLLANFWEETGTRAKN
                     YRDCFTHHVTLADPTWSHSVGMVSLGGAWLPHVPWPDNPVADSDAKFNFEGRLDAIAY
                     CADTHEKSDKFLRHLTKQCLVKWDVGAVLENEFVLANVRIGG"
     gene            1416..1907
                     /locus_tag="VN039_14250"
     CDS             1416..1907
                     /locus_tag="VN039_14250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HWV47159.1"
                     /translation="MATDVYLSIDGIKGESADSAHQGWIELTSAQWGVTQPRSATAST
                     GGGHTAERCEHRTLAISKLADLASPVLMQTCSMGKTIPKARLEFMRADGDGARVKYYQ
                     VELENVMIANMEQMVHGGGILHDEIGLRFSKIKWSYTQQKIGGGAGGSTTGGWDLACN
                     KCA"
     gene            2007..>2232
                     /locus_tag="VN039_14255"
     CDS             2007..>2232
                     /locus_tag="VN039_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47160.1"
                     /translation="MLPQAPMDSGYYVYGELYKKPAKGAYQYAHPIMMTAILRVALEW
                     QAIDNRRIGIGDISLAGGRKTPDHDSHMSGL"
BASE COUNT          545 a          610 c          588 g          489 t
ORIGIN      
        1 gaaaacatca gcaaggcgat aacaagaatg cgatatttca tttcgccccc aatttgatgc
       61 cgacatggaa gtggtcgtca tggcggaccc agtgccttac accgggaata cggttgtcgt
      121 tgaacagcac ctgcttgacc agcgggtgag aaaagaagat agcgatgagc ttcgccgtcg
      181 cttcaccatc atattcgctt tgcatccagg tcactggcag gtgagcaccg tcctttctca
      241 gaggccgtac gtcgacctgc aggccgtctt tgtgcgaatg atgctcgccc gtggattctc
      301 cgttggccat gctgatattc ccgatgccga actgacggct ttcggttgtg gcccactcgc
      361 gctccaccca aaacaacacg ctcatcatcg cgggatgggc atactggtag gctcctccgg
      421 caggggtgcc ataaatgtag taacccgcct caaccgccaa ttctcacgtt cgcgagcacg
      481 aactcatttt ctaagactgc tccaacatcc cacttcacta gacactgttt cgttaggtgt
      541 ctaagaaact tatcagattt ctcgtgcgta tcagcacagt aagcaatcgc atcaagacgg
      601 ccttcaaagt tgaacttcgc atcgctgtcg gcaacaggat tgtcgggcca agggacgtgg
      661 ggtaaccaag cacctcccaa cgaaaccatg ccgacagaat gcgaccaagt tggatcggct
      721 aacgttacat gatgtgtaaa gcagtctctg tagttttttg ctcgtgtccc tgtttcttcc
      781 caaaaattcg ctagcaggcc agctaccact accggattcg tccgggatct atctggtgta
      841 ttgtttatcc gcttgacgaa ggcattgatt gagtgtggcg aatcgtgcgg aacgacttcc
      901 gcgctgatca agaaatccat gacggcatcg tgtgcactac gagtagcctc aagaaaacca
      961 tcgaggtgga agaatagggg ctcagtaagt ggtccaaaat agctggaatt aacagggtct
     1021 tccacgctag ccgttacggg ggccggttgc atcgcatcga ccatctcacg taccgtctgt
     1081 cgagccgcct ttaaatggta ttccgcgcga cggtgcttaa aaaatatttt gccggactga
     1141 agccgccaac tgtgtcgctt acgcttatcc aacttttcag cgattgcctc tactgcgacg
     1201 ctatcccatg tcgggtctgg aagaacaatc attggtgtgg gatgagtgga ggtagagact
     1261 gatactaaac cataatcggc gtcaacgaca tgcgatggta tagaaacctt ttcataccat
     1321 ttgatccttc tcgttccgcc gcttgaccat cgccaacaac ctctcagcag tgccgtacaa
     1381 aatgttcgct tactcaactt gtaccggagg ggagcatggc tacagacgtt tacctgagca
     1441 tcgatgggat caagggcgag tcagccgatt ccgctcatca aggatggatc gaactgactt
     1501 ccgcccaatg gggcgtaacc cagcctagga gcgcaactgc ctcaacggga ggcggtcaca
     1561 ccgcagaacg ctgtgagcac cgaaccctcg cgatcagcaa actcgcagac cttgcgtcac
     1621 cagttctgat gcaaacctgc tcgatgggta aaacgatccc gaaggccaga ctcgagttca
     1681 tgcgcgccga cggagacggc gcccgcgtca agtattacca agtcgagtta gagaacgtca
     1741 tgatcgccaa catggagcag atggtgcacg gcggaggcat cttgcacgac gaaatcgggc
     1801 tgcgcttctc caagatcaaa tggtcctaca cgcagcagaa aatcggggga ggcgcgggcg
     1861 gatcgacgac gggaggttgg gatttagctt gtaacaaatg cgcctgacgg aggccgctca
     1921 tgccaaccga atggacaacc gaagacgacg acaagtgacc cgatcccatc cgtccccggc
     1981 cgcctgagga ttctcgtgga ttcttcatgc ttccacaggc gccgatggac tcgggctact
     2041 atgtatatgg cgaactctac aagaaaccgg caaaaggcgc gtaccaatac gctcatccga
     2101 tcatgatgac tgcgattctt cgcgtggcat tggaatggca ggccatcgat aatcggcgta
     2161 ttggcatcgg agacatcagc cttgcgggcg gcagaaaaac tccggatcat gattcgcaca
     2221 tgagcggact tg
//
LOCUS       DATMTI010000904         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_331102, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000904 DATMTI010000000
VERSION     DATMTI010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331102"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VN039_14260"
     CDS             <1..822
                     /locus_tag="VN039_14260"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWV47161.1"
                     /translation="RPEDVRDKDLANKLGITISNQIVVMFHCGSRGFGHQVATDYLQR
                     FLKVMEPKYGIKILDRELACAPFDSPEGRDYFAAMKCGLNMSFANRQVILHRIREVFS
                     GIFGRSAEDLEMRMVYDVSHNTAKLERHVVDGKEKTLLVHRKGSTRAFGPGHDELPAR
                     YRETGQPVIIGGSMETGSYLLVGTSSGSETFFSTAHGSGRTMSRTKARKLWHGKQLQR
                     DLEARGIYVRSTSWAGLAEEAGGAYKDIDEVIAATELAGINKPVARFTPIGNVKG"
     gene            1010..1960
                     /locus_tag="VN039_14265"
     CDS             1010..1960
                     /locus_tag="VN039_14265"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWV47162.1"
                     /translation="MTPRTPITLITGPLGGGKTTLLRHILEARREKIAIVMNEFGEIG
                     IDTKVIEGKNVQIAELGGGCVCCSLLGEFEAAVKEIIEKIAPERIVVETTGLAEPEAL
                     VFDIEQALPQCRIDGVVSVIDADMLIRFPELGHTTRLQIEGADILLLNKVDLVEATQI
                     EPLETKLRGVNPTATIVRTERCRIDPELLFGIGLDRKLVPSKSGSSSYFAEATADRQI
                     PATAHQHHAEFESFAFTSDKSFSPSCFDDFANGLPKSVVRAKGFIRFEDGSQLFNFVA
                     GRWELEPFEAARTELVFIGKDIAAERKAIVSALDECVVRN"
BASE COUNT          524 a          559 c          629 g          479 t
ORIGIN      
        1 cgaccagaag acgtgcgcga caaggacctc gcgaacaaac tcggtatcac catttccaat
       61 cagatcgtgg tgatgtttca ctgcggcagc cgcggcttcg gacatcaggt cgctaccgat
      121 tatctccaga gattcttgaa agtgatggag ccgaaatacg ggatcaagat cctggaccgc
      181 gagctggcat gcgcgccctt cgattcaccg gaaggccgcg attatttcgc tgcgatgaaa
      241 tgcggattga atatgtcgtt cgcgaatcga caggtcattc ttcatcggat tcgtgaagtg
      301 ttttccggaa ttttcggcag gagtgctgaa gatctcgaga tgcgcatggt ttacgacgtc
      361 tcacataaca ccgcaaagct cgaacggcac gtggttgatg gaaaggaaaa gacgttgctc
      421 gtgcatcgca aaggatcgac ccgagccttc ggtcccggac acgatgagct gcctgcgcgc
      481 tatcgtgaga caggccagcc ggtgatcatc ggcggtagca tggagactgg gtcgtatctg
      541 cttgtgggca caagctcagg ctctgaaaca ttcttcagta cagcgcacgg gagcggccgc
      601 acgatgagcc gcaccaaggc gcgcaaactc tggcacggca aacaacttca gcgcgatctg
      661 gaagcacgtg gcatctacgt tcgcagcacc tcatgggcag gtctcgccga ggaagcaggc
      721 ggcgcttaca aggatatcga tgaggtaatc gcagcgacgg agttggcagg gatcaacaag
      781 ccggtcgcgc gattcacgcc gattggaaat gtgaaaggat gagaggtcca gttcaatacc
      841 gcacacactg tcatgttgag cgaaggcgct gcaacgaaat gcgacgcatg aggccaggct
      901 ttcgaacatc tcagttcatc gtcttcgcga gtttagcagg aatccaggga tccttcgctg
      961 cgctcaggat gacaactagc tgcttggatt ttggtgtttt ctgtaacaaa tgactccgcg
     1021 cactcccatc acgctaatta ccggtccgct tgggggcggg aagacaacat tgctccgtca
     1081 catcctggag gcgcgacgcg aaaaaatcgc gatcgtgatg aatgagttcg gcgaaatcgg
     1141 tatcgatacg aaagtgatcg aaggcaagaa cgtgcaaatc gccgagctcg gtggcggttg
     1201 cgtttgttgc tcgttgctcg gcgaattcga agcagcggtg aaagagatca tcgagaagat
     1261 cgcacccgaa aggatcgtgg tggaaaccac cgggcttgcc gaacctgagg cgctggtttt
     1321 cgatatcgag caagcgctac cgcaatgccg gatcgacggc gtggtttccg tcatcgacgc
     1381 agacatgctt attcggttcc cggagctagg ccacaccact cggctccaaa tcgaaggggc
     1441 agacatcttg ctgttgaaca aagtcgatct tgtcgaagcg acgcagatcg agccgctcga
     1501 aaccaaactg cgaggagtaa acccgaccgc cacaatcgtt cgcaccgaac gttgcagaat
     1561 tgatccggaa ttattgttcg gaattggact ggaccgaaaa ctagtaccaa gcaaatcggg
     1621 atcatcctcc tacttcgccg aggctacggc ggacaggcag atcccggcta cagctcatca
     1681 gcatcacgct gaattcgaat ctttcgcatt cacgtctgac aaaagttttt cgcccagctg
     1741 tttcgatgac tttgcaaatg gactgcctaa gagcgttgtt cgcgccaagg gcttcatccg
     1801 gtttgaggat ggctctcagc ttttcaattt cgttgcgggt cgttgggaac tggagccgtt
     1861 tgaggcggct cgcacggaac tggtgttcat cggaaaggac atcgctgcgg aaaggaaagc
     1921 gatcgtaagt gcgctggatg aatgcgttgt taggaattga tgaagcgcaa tgatctttgc
     1981 aggtcgtttc tgtgaaacgc catcgcgtct gacacagacg ccttgcaatt aaattcgaac
     2041 ttgattcggt attcgacatt gggatttcgt tattctttgc cccatggcgt acacatactt
     2101 ggaggacgtt ggcacagccg atatcgcctt tgaagcgact gggcgcgatc tgcctgagtt
     2161 gttcagcgac gcggctgacg caatgaccaa c
//
LOCUS       DATMTI010000905         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_247314, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000905 DATMTI010000000
VERSION     DATMTI010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247314"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..719
                     /locus_tag="VN039_14270"
     CDS             249..719
                     /locus_tag="VN039_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWV47163.1"
                     /translation="MRNRTTVGAAMVFGLLLSGPLYAADQPGQVGQVSVAGVEGSKGP
                     DGVIGISLHIGAEHVGDPASLYIGRVHREGPAHKAGLRHGDELLSVDGTTVSGKSYEQ
                     LVKMVRGEAGSTVKLQIKREGEESPREISITRVADDSLARRPAEHGLYKDKPKP"
     gene            1039..>1542
                     /locus_tag="VN039_14275"
     CDS             1039..>1542
                     /locus_tag="VN039_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47164.1"
                     /translation="MRQSLGMLTFILCLRLTAVSMAAPAAETGEQDFAKGETLLKKQQ
                     YAEARATLEAGIMKNSSNVQAHFNLAEACRGLAAWACAEEHYETALHLDAKSRTTGMR
                     DLRLRRLEVWRSLEEVTAWRLLEEAKDLIAGGHAEPDQIKRAEEALDGANELGLNQEQ
                     QAVYQQLQ"
BASE COUNT          369 a          372 c          485 g          316 t
ORIGIN      
        1 gtatcggtga agatacagtt cgatctggat tggatacagc tgtcacaaga tgaatttcac
       61 aatgggacat cgggacatct aggaatcccg ctagtagacc tagcaggcgt tcaaaactaa
      121 agaatatgaa caatgatgat acgaccgtcg cggttttggc gacagcaagg gccacgtctc
      181 tgtcagccag gtcgtaaacc gagatttggt cgtctctcac aaactatgtt ggaattggag
      241 gtaggttcat gcggaataga acaactgtgg gagccgcaat ggtgttcggt ctcttgctga
      301 gcggtccgct ttacgctgcg gatcaacccg gtcaggtggg tcaagtttct gtcgcaggcg
      361 tagagggatc gaagggaccg gatggagtga tcggtatttc gctccacatc ggagctgagc
      421 atgtcggcga tcctgcctct ctgtacatcg ggagagtcca ccgtgaaggg ccggctcaca
      481 aagccgggct tcggcatgga gatgagttgt taagcgtcga tggcacgaca gtgtcgggga
      541 agagctacga gcaactggtc aagatggttc gtggagaagc aggaagcacg gtgaagttgc
      601 agatcaagcg agagggtgag gaaagtcctc gggagatttc catcacacga gtcgccgatg
      661 acagcctggc gaggaggccg gcggagcatg gcctctataa ggataagccg aaaccttaga
      721 acgaggatgc tgcaacagcc ttctagacgc gttctcgcct cgggtcatcc ttaggataaa
      781 gcgtgagcgc gcctgaggga tagtgccggc gcaccgcaca cactggtggc ccaccatttc
      841 gcgccgtgcg ccttattcgt cacctcgcgc ctatccacaa attggaaaag ctctgtcgat
      901 cgcgtaagct ctttatgctt acgcttcact atgctatgtt cccggcaacg aaaactgtgt
      961 tcacgacggc ggccggtgtt ctctaccggg aggcgagact cactgccgga ggcagtcaca
     1021 attgggagga aagtatttat gaggcagtcc cttgggatgc tgacttttat cctctgcttg
     1081 agactgaccg ctgtgtccat ggcggcgccg gcagccgaga ccggcgagca agactttgcc
     1141 aaaggggaga ctctactgaa gaagcagcag tatgcggaag cacgtgcgac gttggaagcc
     1201 ggcatcatga agaactcctc gaacgtacaa gctcatttca acctggcgga ggcctgtcga
     1261 ggtctagcag cctgggcctg cgcggaagaa cattacgaga ccgcgttgca tctggatgcg
     1321 aaatccagga ccaccgggat gagagatctg cggttacgca ggttggaggt gtggcgttcg
     1381 ttggaggaag tgacggcgtg gcggttgctg gaggaagcga aagatttgat tgccggtgga
     1441 catgctgaac ccgaccagat caagagggcg gaggaagctc tggacggcgc caatgaacta
     1501 gggctcaacc aagagcaaca ggctgtctat cagcagttac aa
//
LOCUS       DATMTI010000906         1544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_543634, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000906 DATMTI010000000
VERSION     DATMTI010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543634"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VN039_14280"
     CDS             <1..343
                     /locus_tag="VN039_14280"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV47165.1"
                     /translation="SPERGRIVSKLTHPHLPSWDEWLELATGGDTQTQIGHKLGVSRA
                     TVARWSRRRVPDPNTVLALARAYKADPIRGLLSSGWLKASDLNNGGMRYVVSFAPTEV
                     LVNELHTRLGS"
     gene            complement(322..>1544)
                     /locus_tag="VN039_14285"
     CDS             complement(322..>1544)
                     /locus_tag="VN039_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWV47166.1"
                     /translation="GHVMARNGSGVSIFHSEKKGLWVARIELPRRNGERRRKEVTAKT
                     HARLLEKLRGPLKQYHLTGDMPTSSTRLGDYLEYWFENVVLKERTPNTAAGYRSVLWT
                     HVIPEIGNVALDKLTTAHLRRVHTRITSTEKGAHDPPVYLSSTYALNAHRVLSAALTS
                     AERDGLISRNPAALMAAPRKSLKKIDALTVSEAKDVLKLVVKEFGKTGEYDPRPARWA
                     TYLLTGARRGEILGLEIDRVTDVLELSWQLQRVANIETAPTDYEYRHIAKGMYWTRPK
                     SSAGWRVIPLVEPLKSILETHLAVMGHNDYGLVFASGKGLPIDPDTETELWERALEKS
                     GITTKHVRLHDLRHSTVDLLYGAGVSEDLIVEIIGHSTRGVTRGYKSPANMKRREAAM
                     LQLSSFLGYEPKRV"
BASE COUNT          339 a          490 c          386 g          329 t
ORIGIN      
        1 gtcgcctgaa agggggcgaa tcgtgtcaaa gttgacacac ccgcatttgc cgtcatggga
       61 cgaatggcta gaactcgcca caggcgggga cacgcaaacg cagatcggac acaaacttgg
      121 ggtatcccga gcgaccgtag cccgctggtc acggcggcgc gtccccgacc ccaacactgt
      181 gcttgccctc gcccgcgcat acaaagcgga cccgatacgt gggcttctgt catcaggttg
      241 gttgaaagca tccgacctga acaacggggg catgcggtat gtggtcagtt tcgcccccac
      301 ggaagttctt gtgaacgaac ttcacacccg tttaggttca taaccaagga acgacgacag
      361 ttgcagcatt gccgcttcac gacgtttcat gttcgccggc gacttgtacc cgcgtgtgac
      421 accacgtgtg gagtgcccaa tgatttccac aatgagatct tcggacacac ccgccccgta
      481 cagcaggtca acggtcgaat gccgcaggtc atgcaaacgc acatgtttag ttgtgatgcc
      541 ggatttctct agggcacgct cccacagttc cgtctccgtg tcagggtcaa tcggtagacc
      601 cttaccgctt gcgaaaacca acccgtaatc gttgtgcccc atcaccgcca agtgtgtttc
      661 aaggatcgac ttcaacggtt ccaccaacgg gataacacgc cacccggcag acgacttcgg
      721 gcgcgtccaa tacatgccct tcgcaatatg ccggtactcg taatcggttg gtgctgtctc
      781 aatgttcgcc acacgttgca actgccacga taattcgagc acatcggtta cccggtcgat
      841 ttcgagcccc aagatctccc ctcgacgtgc ccccgtcagg agatatgttg cccacctcgc
      901 gggtcgcgga tcatactcac ctgtcttacc aaactctttc accacaagct ttagcacgtc
      961 tttcgcttcc gagacggtga gcgcatcaat cttcttcaaa gacttccttg gagccgccat
     1021 gagcgccgca ggattgcggg aaataagacc gtcccgctcc gctgacgtaa gcgccgcaga
     1081 gagcaccctg tgggcgttca gagcatacgt tgacgacagg tacacgggcg ggtcatgcgc
     1141 gcccttctcc gtggacgtga tacgggtgtg aacacgccgc aaatgggctg tcgtgagctt
     1201 atccaacgcc acattcccaa tctcgggaat cacatgagtc cacaacaccg accggtaacc
     1261 tgccgccgta ttcggtgtgc gttccttcag cacaacattc tcaaaccagt actcaagata
     1321 gtcacccaac ctggttgacg aggtaggcat gtccccagtc aagtggtact gcttcaacgg
     1381 gccacgcaac ttctcaagca gccgcgcatg agttttcgcc gttacctctt tacgcctccg
     1441 ctccccgtta cgtctgggta gttcaatacg tgcaacccac aaccctttct tctcactgtg
     1501 gaaaatgctc acaccggaac cgttgcgtgc catcacatgc ccca
//
LOCUS       DATMTI010000907         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_458516, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000907 DATMTI010000000
VERSION     DATMTI010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458516"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..541
                     /locus_tag="VN039_14290"
     CDS             68..541
                     /locus_tag="VN039_14290"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWV47167.1"
                     /translation="MKNDGFSLIEVMVAMVIAGVALMGTLGAVEVSSRHVQQGGLNSK
                     AIELAQARLEAKRSIRWQSILEDDLDRDGIPETFMADDGQGSDITADDGIYTAMFKRD
                     GVTVVWMIEAERPGPLFSAAVVMIRAVASYPGPNGHKKEVQVATIRANPNFVGER"
     gene            538..1257
                     /locus_tag="VN039_14295"
     CDS             538..1257
                     /locus_tag="VN039_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47168.1"
                     /translation="MNQGDVTGSERGICLTELMVSLTAGAIVLAAALNALNIAQAHVS
                     KQHNDLRHQQDLRLGLEVFEQEVRLAVADSILSATPDEFRFHANVSAYRTVTTGAVVP
                     GQSVVAVQDGSGWSEGKAVIICGHQACETHRLSRSGQHYQLTLTEPVTLSFPTGSSVE
                     VSNRVVYYTKRDENGPVNLMRMVDGGASLLIGDLDNLHFTYWDEHGHSTQQPSLVKRV
                     VLEIESNHSLHRMMREVSLRS"
     gene            1268..2737
                     /locus_tag="VN039_14300"
     CDS             1268..2737
                     /locus_tag="VN039_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HWV47169.1"
                     /translation="MLYFNIGDREQGIALLGAMIVALILSLLGATLLNLAGQEAASAG
                     LASQAAVAQQLADAAGELVVAWFHSPRAIAGVPQLSALREKRNRDKDGAPSFLDPSGR
                     SQFVGTADQPDVWLQIGNLSDNRLLNDPETGMFRTMTHLGQVEELKVYAPSKPGLLCT
                     IDTTVVTNTNPPVRQSILLQLGAMDLPPLRAAVQVDQYLGRFQQGAESPVSVHWGELK
                     VGGDLVLAQAAEIPLRSALAPVTGQPYDEVTQREDQWMEGWIGGTVQVTHLPSEQNPS
                     FPSNLHMGQNPIPGVRLDQWPYEQIKRVAKRFGRYFAIDREGLLYPQGIVEPGRGISP
                     DEVFRSQGSGDQLGLIFIDTLDQTAPRPDNLGVVRLHVPYFEGMAVVQGHVVLTPGGS
                     GQSVQALSPPPMDQAHEVVRMPVQLSGMHFNGVLYASGDITVAGKVRLYGAVTAGGTI
                     TSAGSGSSLEVWYDHDLSRGLYRGLPVVYRAPGTWMTRY"
BASE COUNT          713 a          720 c          861 g          636 t
ORIGIN      
        1 tagaaggaca gacccaacag ctcaccgtga gcatcacggg tcgggtatcg attctatgag
       61 acgaacgatg aagaatgatg gtttcagcct gatcgaggtg atggtggcaa tggtgattgc
      121 aggcgtggcg ctgatgggca ctctgggggc agtggaggtt tcttctaggc acgtacaaca
      181 gggaggtctg aacagtaagg caatcgagtt ggcccaggca aggttagaag cgaagcgttc
      241 cattcgctgg cagtccatcc tggaagatga tctcgatcga gacggtattc cagaaacgtt
      301 catggctgat gacggccaag gctcggacat aactgcggat gatgggatct acacagccat
      361 gtttaagcgt gatggagtga cagtggtgtg gatgattgaa gcagagcggc caggaccact
      421 gttctccgcc gcggtagtga tgatccgagc ggtcgcatcc tatcctgggc ccaatggaca
      481 caagaaagaa gtgcaggtcg caacgattcg agccaatccg aactttgtgg gggaacgatg
      541 aaccaggggg atgttaccgg ctctgagcgc ggtatttgtc tgacagaatt gatggtcagt
      601 ttgacggccg gagcgatcgt attagctgca gcattgaatg cattgaatat cgcgcaggcc
      661 catgtgagca aacagcacaa tgacctgaga catcagcaag atctccgact gggactggag
      721 gtattcgaac aagaagtccg cttggcggtg gccgattcga tcctctcagc gactccggat
      781 gaatttcggt ttcacgccaa tgtcagtgct tatcgaacag taaccaccgg tgcggtcgtg
      841 ccgggccaat cagttgttgc ggtgcaggat ggcagcggat ggagcgaggg gaaggcagta
      901 ataatttgcg gccaccaggc ctgcgaaaca caccggctct cccgttcagg gcagcactat
      961 cagctgacct tgactgaacc ggtaacgttg tcgtttccca ctggttcctc ggtggaggtg
     1021 agcaatcggg tggtctatta caccaagcga gatgaaaacg gaccagtgaa cctgatgcgg
     1081 atggtagatg gaggagcaag tcttctcatc ggcgacctgg acaatctgca ttttacgtat
     1141 tgggacgagc atggtcacag cacccagcag ccttctctcg tgaagcgagt cgtgctcgaa
     1201 atcgaatcca atcactccct gcatcgaatg atgcgggagg taagccttcg atcataggag
     1261 taaagacatg ttgtatttca atatcggaga tcgtgaacag gggattgcac ttctgggagc
     1321 catgatcgtt gctctcattc tgtcgctgtt gggcgcgaca cttctcaatc tcgccggaca
     1381 agaggcggca agtgctggat tagccagcca ggcggcggtt gcccagcaac tggccgatgc
     1441 ggctggtgaa ttggtggtgg cctggtttca cagcccccga gcaattgcag gcgtacccca
     1501 gctttcggct cttcgtgaaa aacgaaatag agataaagac ggagcgccat cctttctcga
     1561 tccgtccggt cgctcgcaat tcgtcggtac ggcggatcag ccggatgttt ggttacagat
     1621 cggcaatctc tcagacaacc gactgttgaa cgatccagag accggcatgt ttcgcacaat
     1681 gacacatcta gggcaggtgg aagaactcaa agtctacgca ccttccaagc cgggtctcct
     1741 gtgcacaatt gacactacgg ttgtgaccaa taccaatccc ccggtcaggc aatcgatact
     1801 cttgcaattg ggcgcgatgg acttgccgcc gttacgagcc gctgtgcagg tggaccagta
     1861 tctcggccgt ttccaacaag gagccgaatc accggtctct gtgcattggg gggagttaaa
     1921 agtaggaggt gatctggtcc tcgcgcaggc tgctgagatc ccattgagga gtgcgcttgc
     1981 gccagtaaca gggcagccat atgacgaagt gacgcaacga gaagatcaat ggatggaagg
     2041 atggattggc gggacggtgc aggtgacgca tttaccatcc gaacagaatc ctagtttccc
     2101 cagcaacctt cacatgggcc agaatccgat tccgggagtt cgcctcgatc agtggcccta
     2161 tgaacagata aagcgagtgg caaaaagatt cggccgttac ttcgcgattg atcgagaagg
     2221 tcttctctat ccgcaaggca ttgtcgaacc aggacgtggc atttctccag acgaggtatt
     2281 ccggtcacag ggaagcggcg accagcttgg tctgatattc atcgacacgc tcgaccagac
     2341 ggctccccgt ccagacaatc tcggggttgt cagattgcat gttccgtact ttgaaggcat
     2401 ggcggtcgtg caaggacacg ttgtgcttac tcccggtggg tcaggacagt cagtccaggc
     2461 acttagccct ccaccaatgg atcaggctca tgaagtcgtt cggatgcctg ttcaactctc
     2521 cggcatgcat ttcaacgggg tgctctacgc aagtggagat atcacggtcg ccggaaaagt
     2581 cagactatac ggagccgtaa cagcaggtgg gacaatcacc tccgctggtt caggaagcag
     2641 cttggaggtg tggtatgacc acgatctcag tcgtgggtta tatcgtggat tgcccgtggt
     2701 ctatcgtgcc cctggtacct ggatgactcg atattgagcc atagatgaag atcgaacgat
     2761 atcagtgtga aggagagtgc cacataaaaa caacttctta ttgtcgatga gaaaggatga
     2821 gatagaatga tgacctcgca aagcgttgaa acgcatccca aacggaagat cctctcggta
     2881 gccagtctaa aaaagtcgtc gatggccaaa caccctcgca agatgacgat
//
LOCUS       DATMTI010000908         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_287925, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000908 DATMTI010000000
VERSION     DATMTI010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287925"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VN039_14305"
     CDS             <1..782
                     /locus_tag="VN039_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47170.1"
                     /translation="AIERAFVAQNGVDEDGVFLDSFVPREGMKYFIHVDLAQKHDHCV
                     VSMAHVEKWINVKIGADYEEMHPIVIIDCIRWWTPSKTKTVDFADVRDFIVALKRRGF
                     DIKRATFDRWNSHDTMNILENEFGIKTDILSVDKKHYDDYLSIMYDKRLIGPKVQLLI
                     DELGELRLVQKVTKVVIDHPRKGTKDFSDSTCGAIFDAVSLTPKPQSEVVEVVTLADL
                     AKREREAEAARRKEQDFNGVIRPPRQMSDELREFLEGARLL"
     gene            845..1474
                     /locus_tag="VN039_14310"
     CDS             845..1474
                     /locus_tag="VN039_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47171.1"
                     /translation="MYVQMEGESVVSVSIDQKQDYTKVPRAELIRQLIERDGTRCQYP
                     SHNHELDFSVKDGPQEVTIDHWYSQSFGKQNGWTIDQIWAVSNLKLMCKKGNASKGDL
                     IPNEDGTLPAKPQSTFRFRRQKRANRGTYCDQCDNGHNLFMGEICASCGGNAQNFPRW
                     AKERAPDCDHSLSWCWACSIGIIERPSSIGIAMRQADSTELGEILDDEE"
BASE COUNT          450 a          370 c          424 g          371 t
ORIGIN      
        1 aagcaatcga gagggcattc gtggcccaga acggggtaga tgaagatggc gtatttctcg
       61 atagtttcgt tccacgcgag ggtatgaaat atttcatcca tgtagaccta gcacagaagc
      121 acgaccactg cgtagtaagc atggcccatg tagagaaatg gataaatgtc aaaataggtg
      181 ccgactatga ggagatgcat ccaatagtta taatcgactg cattcgctgg tggactccat
      241 cgaagaccaa aactgtagac ttcgccgacg tcagagactt cattgttgca ttgaagaggc
      301 gtggatttga tatcaaaagg gctaccttcg acagatggaa ctcgcacgac accatgaata
      361 ttctggaaaa tgagttcggt atcaaaacag atatcctttc ggttgacaag aaacattatg
      421 atgattacct ctcaatcatg tatgacaaga ggctcatcgg gccgaaggtt cagttgctca
      481 tagacgagtt gggagaactc aggctcgtcc agaaggtcac gaaggttgtc atagaccacc
      541 ctcgcaaggg tacgaaggac ttctcagact ctacttgcgg agccatcttc gatgctgtat
      601 ccttgactcc gaagccacag agcgaggtcg ttgaggtcgt cacgttggca gacttggcta
      661 agcgtgaacg tgaggctgag gcggcgcgac gaaaagaaca agatttcaat ggagtgattc
      721 gcccacctcg acagatgtca gatgaattaa gagagtttct agagggcgcc aggcttcttt
      781 gacgcctgtg ctccactgtg ctatactacc actaagatgc atgctatcat aggcgataac
      841 gaatatgtac gtccaaatgg aaggagagag tgtcgtgtct gtatcaatag accaaaagca
      901 agattacact aaagtgcccc gcgcggaact gattcgtcag ttgattgaac gcgacggcac
      961 gcgatgccag tatccctccc acaaccatga actagacttt tccgttaaag atggtccgca
     1021 ggaagtcacg attgaccact ggtacagcca gtccttcgga aagcaaaatg gctggacgat
     1081 agaccagatt tgggccgtca gcaatttgaa attgatgtgc aaaaagggga atgcatctaa
     1141 gggcgacctg attccgaacg aggatggcac acttccggca aagccacagt ctacattcag
     1201 attcaggcgg cagaagaggg ctaacagggg cacctactgt gaccagtgcg acaatggcca
     1261 caacctgttc atgggtgaga tttgtgcatc ttgtggcggc aacgcccaga actttcctcg
     1321 ttgggccaag gaacgtgctc ccgattgcga ccactcgttg agttggtgtt gggcctgttc
     1381 cattggtatt atcgaacgcc cctcctctat cgggattgcc atgcggcagg ctgactctac
     1441 cgagttgggc gaaatcctag atgacgaaga gtgaactcat cgcatatcta gaaactctgc
     1501 atcaccagat tctagacttg gcgatgaaag tcaaagagtt ccaagtcata gtctaccaac
     1561 agactcttga caacgacgat gatgatgtgt tagactagac tagagtctgt ggtgt
//
LOCUS       DATMTI010000909         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_75665, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000909 DATMTI010000000
VERSION     DATMTI010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75665"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="VN039_14315"
     CDS             complement(<1..2010)
                     /locus_tag="VN039_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWV47172.1"
                     /translation="MKTIRCLIVTALPGFFIVADAMFSDSTMRGVSRHSAKVSGQTSP
                     SFEVAPQASTGLAPYAVELADMDGDGDLDFVTSNLVDQESSTVSVAKNTGDGTFAPPA
                     HYPVGPGPTDLRLADFNGDGKPDVVCLAALSGDFGESYATVLFNDGTGGLINRHDYPV
                     GENANSGGLDVGDYDGDGHRDFAVASLMTGVYVYRNTGNGDFALWTQIAVALGPTHIA
                     SADFNGDGHLDLVVGNVDAAQVYLNNGTGFTAGVFLDNFPDTVQGIATGDFDDDGKPD
                     FALTGRRLSVYRNLGGGASFAKAAYLAGENQVGIKTADMDGDGNLDITVSNYLANSVS
                     VYSNDGTGHFPNKREWGVGWSPNSHGIGDVNADGKPDIVAAASQLSKTTVNVVLNAGQ
                     RNYVARRDYGMLGGAWGVGIADFDRDGDLDVVSPAAISNADGPFIFYGNPDGTLQDGI
                     QIENWGNNFPTDVAVGDFNDDGWPDFVTSIFSPGNRIRVSINQGNGTFLPSVSYEAGG
                     NPSGVGIGDLNADGNLDVVNSNASQFDNTISVFIGNGDGTFQPQVVVPVGLRPSDVLL
                     ADFDKNGRSEVIVTHFGSTAIYYFKPDAQGVLGPPEIIDLGSTHNNAVAADFDNDGWL
                     DAMVAAGHAVFLRNNQGGGFLPPVTTSVPAGYVEAGDWDQDGLMDI"
BASE COUNT          484 a          662 c          653 g          475 t
ORIGIN      
        1 gatatccatg agtccatcct ggtcccagtc tccagcctcg acatagcctg ccgggacaga
       61 cgtggtaacc ggtggaagga agccgccgcc ctgattgttg cggaggaata cggcatgccc
      121 cgcagcgacc attgcgtcga gccatccgtc gttgtcgaag tcagccgcga cggcgttgtt
      181 gtgggtcgag ccgagatcga tgatctcagg cggccccaac acaccctgcg cgtccggctt
      241 gaagtagtag atcgcggtgg atccgaaatg ggtgacaatc acctcgctgc ggccattctt
      301 gtcgaagtct gccaacagca cgtcgctcgg gcgtaacccg acaggaacca cgacctgggg
      361 ctgaaaggtt ccgtcaccgt tgcctatgaa gacggagata gtgttgtcga actgcgatgc
      421 gttcgagttt acgacatcca gatttccgtc tgcgttgaga tcgccaatgc ctactcccga
      481 cggattgccg ccggcctcgt agctgacgct cggcagaaag gtgccattgc cttgattaat
      541 gctcacgcga atgcggttgc cgggactgaa gatcgaggta acgaaatcgg gccagccgtc
      601 atcgttgaag tcgccaaccg ccacgtcagt tggaaagttg ttgccccaat tctcgatctg
      661 gattccatcc tggagcgtcc cgtcgggatt gccgtagaag atgaatggcc catcggcgtt
      721 tgagatggcc gctggcgaga cgacatccag atcgccgtcc cggtcaaagt cggcaatgcc
      781 cacaccccag gcccctccga gcatcccgta atcgcgtcgc gcaacgtaat tccgctggcc
      841 cgcgttcagc accacgttaa cggtcgtttt gctaagttgg ctggccgcgg ccacgatgtc
      901 tggcttgccg tctgcgttaa catctccgat cccatggctg ttcggcgacc aaccaacgcc
      961 ccactctcgc ttgttaggga agtgccccgt gccgtcgttc gaataaaccg agaccgagtt
     1021 ggcaagatag ttcgacaccg tgatatcgag gtttccgtca ccgtccatat cggccgtctt
     1081 gatgccgacc tgattttcac cggcgaggta ggctgccttg gcaaagctcg cgcctccgcc
     1141 aaggtttcga taaactgaga gccggcgtcc ggttaacgcg aaatccggct ttccgtcgtc
     1201 gtcgaaatcg ccggtagcta tgccttggac agtgtctgga aagttatcga gaaagacgcc
     1261 agctgtgaag ccagtaccat tgttcaagta gacctgtgcc gcatcgacgt tccctacgac
     1321 aagatccagg tgaccatcgc cgttgaagtc cgcggaggcg atatgggtgg gtcccagggc
     1381 gacggcaatt tgtgtccaga gcgcgaagtc gccgttgccg gtattgcgat aaacgtagac
     1441 accagtcatc aatgacgcga ccgcgaaatc acggtgcccg tcaccgtcgt agtcgccgac
     1501 gtcgagtcca ccggagttag cgttctctcc gaccggatag tcatgacgat tgatgagccc
     1561 gcccgtgccg tcattgaaca ggacggtggc gtagctttca ccaaagtctc ccgacaaagc
     1621 ggcgaggcag accacatcag gctttccatc gccattgaaa tcagcaaggc ggagatcagt
     1681 gggaccgggc cccacgggat aatgcgccgg cggagcgaac gtcccatccc cggtgttttt
     1741 ggcgaccgaa accgtgctgg actcctgatc gaccaagttt gaggtcacga agtccaaatc
     1801 gccatcgcca tccatgtcgg ctaactcaac cgcgtaaggt gcaagacccg tcgaggcttg
     1861 cggcgcaacc tcgaagctgg ggctcgtttg cccgctcacc tttgccgaat gcctcgagac
     1921 cccgcgcatt gtgctgtctg aaaacatcgc atccgcgacg atgaagaaac cgggtagagc
     1981 agtaacaatt aagcaacgga tcgttttcat agggttagcc aggttgtatg cgaatcgcaa
     2041 cgggcctcgt ccagaggcgt gagagaaaag taacgccgtc gttaactttg aaggtttgga
     2101 aagtgagagg ttgcccgtcg cgttggcgaa ctacaggggt aagtcacacg ttccgcctga
     2161 gttagaatgg ccaaatggat tcgctctgtg cagttcagcg tctcctggtg ttcgatcaac
     2221 tgatcgcgat ctgcaaaagc tcgagataaa ccccccggct cgtttcttga gatt
//
LOCUS       DATMTI010000910         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_711972, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000910 DATMTI010000000
VERSION     DATMTI010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711972"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VN039_14320"
     CDS             <1..274
                     /locus_tag="VN039_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47173.1"
                     /translation="IRGGASSLRRSSLTLTSSSPQWWCFPGKLARAVQHAAAFSNHMT
                     HSWLPAGRQPPQSRRETDPLIILHIDKRANVCQQVNTGPVLLMSIL"
     gene            complement(591..1364)
                     /locus_tag="VN039_14325"
     CDS             complement(591..1364)
                     /locus_tag="VN039_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47174.1"
                     /translation="MRMTWTNTLRCFLASCVMSGLVAVACGGGYDDRDFSSLPQPTAI
                     SAEGRWTGATPTSRTVAGLVLDDGSYWVFYTTRGNPNVLAGLVQGTGTSHSGAFGSSN
                     TRDFNLEGPGMLAATMSGTYVPKKSFHGTIAYFNGDTESFTSTYDADSESAPNLNLVA
                     GTYAGLRADNHTVTVTVDSAGTLSGHSSDGCIFAGTLSPWAKGNVFHHAVTFGGGACR
                     QGTETVTGVAFYDAAINRLYSAALNSARTTSSIFIGTKR"
     gene            1836..2033
                     /locus_tag="VN039_14330"
     CDS             1836..2033
                     /locus_tag="VN039_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47175.1"
                     /translation="MGQTAKRTLQELRRALKRSQVIMAKTLGFNKLPCHGSRTGATDA
                     SLASARLRYCRRFVRSRSYEQ"
BASE COUNT          514 a          707 c          677 g          528 t
ORIGIN      
        1 aattcgtggc ggcgcttcgt ccctacgccg ctcgtctctg actctgacct cttcttcacc
       61 acaatggtgg tgcttccctg gcaagcttgc gagagccgtt cagcatgccg ctgcgttcag
      121 taaccatatg acgcatagtt ggttgcctgc tggccgacag ccacctcaaa gccggcgcga
      181 aaccgacccg ttgatcatcc ttcacatcga caagcgggcg aacgtttgtc agcaggtgaa
      241 cacaggacct gtactcctca tgtccattct ctgatcgtgc tgtgctctcc gtgtgtgaga
      301 gccggcttgt ggtctattgc ctgtgcgcat ccggccttcg cctggcttcc caccggcggg
      361 ctgcgtcgat catgcaggcg cacccacgtc gtgcccacgc tgaaacagga cttgaaggag
      421 tttgctcaga gctggtttgc caatctgaaa gatcagggat tctttgcacc caccgctgtt
      481 cgagagatcc tgtccccgta atctcgttga tacattgacg gggtatgagg tgcggcgatc
      541 atgcttccgt caaaaaagga aatcctgaca gaaccttgaa ccggtgatgc ttaccgcttc
      601 gtgccgataa agatagagct ggtggtcctg gcactgttga gggccgcact gtacaaccgg
      661 tttatcgctg catcgtaaaa ggccacccca gtgaccgtct cggttccctg gcgacaggcg
      721 ccacctccga atgtcaccgc gtgatgaaac acattgccct ttgcccaggg agacaatgtc
      781 cccgcaaaaa tgcatccatc tgatgaatga cctgaaagcg taccggctga atccactgtc
      841 accgtgaccg tgtggttgtc ggcacggagt ccagcatagg tcccggccac cagattcagg
      901 ttcggggccg actcagagtc cgcatcgtac gtactcgtaa agctctctgt gtcgccattg
      961 aagtacgcga tcgtcccgtg aaagctcttt ttcggcacat aggtgccact catcgtcgcc
     1021 gcgagcattc cagggccttc caaattgaaa tccctggtgt ttgaggaacc gaaggcgccg
     1081 gagtgtgacg tgccagttcc ctgaaccagt ccagccagga cgttgggatt gcctcttgtc
     1141 gtatagaaca cccaatacga gccgtcatcg aggacaagtc cagcgacggt gcgactcgtg
     1201 ggagtcgcgc ccgtccagcg accttcagcc gatatggccg tgggctgcgg cagagaagaa
     1261 aagtcgcgat cgtcataccc gccgccgcag gcaacggcaa cgagtccact catcacgcag
     1321 cttgccagga agcatcgaag agtattcgtc catgtcatgc gcatgggtgt ctccccaagg
     1381 tgagagcgac atgcacgggg ggtgtcttgt gtcccagtat aacaagaaga taatccccct
     1441 caattgggga gagagaacac ggtgagccca gatcggacaa cgaacctcga gtggctagaa
     1501 ccggacgagc cgactctcga tggcggcatg cccggtttga cggaggctcc acggaccggc
     1561 cgatccgggc ccctttctgg cgtcttttgc acagcgtgaa aggttggtct gtggccttga
     1621 tgcgagtgtg cgtattcaag gagcgagact gattttaaga ggttgcagga tatggctttg
     1681 cacgtagttg ccttacatta tgtttttatt gtattgttgt gcccatgttc tttgcccctc
     1741 cgatcatcgc ccggactctc ctccaatgac cagaatcaga caccggtcgg tgacaggtgg
     1801 ggggcgtctc gactgggatt ggtgcaggtg agctgatggg gcagacggcg aaacggacgc
     1861 tccaggagtt gcgtcgtgcc ctcaagcgat cgcaagtcat catggccaag acgctcggct
     1921 tcaacaagct gccgtgtcac ggctcgagaa ccggagcgac tgacgcctct ctcgcttccg
     1981 ctcggcttcg ttactgccgc cgtttcgtca gatctcggtc ctatgagcag tgacggccca
     2041 ggtgtcacac gggtctccca gacaggcgaa acgagcgaca ccgacaagga ccggcgagac
     2101 gcgcgcttcg cacgaatggt caatctgctt catgagaatc cgacgcatct agatattaac
     2161 cctacgtgtt ccaggcgtgt ttcttcacag cacaattggc cccaattccc ctgaattgga
     2221 ctggccagaa ctgagtccga aaaacagcca catgccgctc ctggaggatg tttgcctacg
     2281 cagacaatgg tttggggaga tatctctgaa aggttacaaa accgctgctc tgccgattga
     2341 gctacgccag cccgctcgtg aaatcgcgtt cgaactttcg tcccgcacag taccaacagg
     2401 ccgaagaacc tgtcaagaaa ggtccc
//
LOCUS       DATMTI010000911         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_670161, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000911 DATMTI010000000
VERSION     DATMTI010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670161"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..988)
                     /gene="panC"
                     /locus_tag="VN039_14335"
     CDS             complement(95..988)
                     /gene="panC"
                     /locus_tag="VN039_14335"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWV47176.1"
                     /translation="MKIIRSLTTMTAWSEQLRREGVRIGLVPTMGALHEGHRALIRVA
                     RLRCDALVVSIFVNPTQFGPNEDLAKYPRPLSRDRALCKKEGVDVCFEPTVEAMYPSG
                     FQTTVALPAITRRWEGEVRPHHFSGVATVVTKLFGIVRPRIALFGQKDFQQSALVRQL
                     VKDLDLGVEIVVHPTVREADGLARSSRNIYLSPDDRIRAATLYKSLQAGAEAIRRGVT
                     DGATIQSEMAQVLKKEPAITIDYLAVCDPDTLEPLSSVRSRVVLLGAVRLGSVRLIDN
                     LLVTTPRRATERIERFSREKL"
     gene            complement(985..1539)
                     /gene="folK"
                     /locus_tag="VN039_14340"
     CDS             complement(985..1539)
                     /gene="folK"
                     /locus_tag="VN039_14340"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWV47177.1"
                     /translation="MKETVFIGFGSNVGDRVDFCDRAVTLLGLLPHSRLQGVSLLYET
                     EPVRDRTDPGEGWFLNGVVQLETDIRPRSLLTTLQEIERSLGRDDDNRSGPRTIDLDI
                     LFYGEHVIKEPGLTIPHPRLHQRRFVLMPMNELDPLWVHPTLKQSVAQLLTGVKDQSQ
                     VRFLFPQPSTRYGSRPACGSPSGS"
     gene            complement(1627..>2373)
                     /locus_tag="VN039_14345"
     CDS             complement(1627..>2373)
                     /locus_tag="VN039_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWV47178.1"
                     /translation="ISHIMPLTKTIGFLPDLGAIPKEVAKKAKLMWLNSPNNPTSVIM
                     TKDYFKWVVDFAQEHQIIVCHDAAYSEVYYDGRRPASFMEVEGAKDVGVEFHSLSKTY
                     NMTGWRIGFAVGNKEVLAGLGKVKSNLDSGCFEAVQEAGITALGLDDSMTDGLRKNYQ
                     ARRDTLIPGLKSLGLEVDPPPAAFYVWVTVPKGYTSTSFTAHLLEKTGIVTTPGNGFG
                     APGEGYIRMTLCTPKERLAEAVERIRKAGF"
BASE COUNT          538 a          694 c          634 g          507 t
ORIGIN      
        1 atcgatgggt attcgattcc tcgaaatggt agcagatgac aagaaccgcc tcagtcaggt
       61 aatcctcagc ttgctggtgg gacaaagcgc caacctacag cttttctcgt gagaatctct
      121 cgatacgctc agttgccctt cgtggagtgg tgaccagaag attgtcgata agacgaacgg
      181 agccaagccg caccgcgcca agaagcacaa ctcgcgatct cacggaggaa aggggctcta
      241 aggtatccgg atcgcagacg gccagatagt cgatcgtgat ggccggttct ttcttgagaa
      301 cctgagccat ttctgactgt attgttgccc cgtcggtgac gcctcttcgg atcgcttcgg
      361 cccctgcttg caggctcttg tacaacgtcg cggcacgaat tcggtcatca ggcgacaggt
      421 agatattgcg tgagctcctc gcgagtccgt ccgcttcgcg caccgtggga tgcacgacaa
      481 tctccacacc gaggtccaga tccttcacca gctgccggac gagtgccgat tgttgaaaat
      541 ctttttgccc aaagagcgcg atccgaggac ggacgatccc gaaaagtttc gtcacgacgg
      601 tcgccacgcc ggaaaaatga tgcggacgca cctccccttc ccaccgacgt gtaatcgccg
      661 gaagtgccac cgtggtttga aatccgctcg ggtacatcgc ctctacggtc ggctcaaaac
      721 agacatcgac gccctccttt ttgcataaag ctcgatcccg cgagagaggg cgtgggtact
      781 tggccagatc ttcattggga ccgaactgcg tgggattcac gaagatactg acgacgagcg
      841 catcgcatcg caatcgcgcc acccgtatca acgcccgatg accttcgtgc agcgccccca
      901 ttgtcggaac gagtccaatc ctcacaccct cacgcctgag ttgctcgctc caggctgtca
      961 tggtcgtaag tgaacgaatg atcttcatga gccggacggt gagccgcagg caggacgcga
     1021 gccataccgt gtagaaggct gggggaaaag gaaccgcacc tgagactggt ccttgactcc
     1081 tgtcaataac tgcgccacag actgtttgag cgtcggatgg acccacagcg gatccaactc
     1141 attcatcggc atgagcacaa atcgtcgctg atggagacga ggatgcggaa tggtcaaccc
     1201 tggttccttg atcacatgtt cgccataaaa gaggatgtct aagtcgatcg tacgaggacc
     1261 ggaccggttg tcatcatccc ggccgagaga gcgctcgatt tcctggaggg tcgttaagag
     1321 actacgtggc cggatgtccg tttcaagctg caccacccca ttcaagaacc agccctcccc
     1381 gggatcggtc cggtctcgaa ccggttctgt ttcatacagc agtgaaacgc cttgaagccg
     1441 agaatgtggg aggaggccga gcaacgtcac cgcccgatcg caaaaatcga ctcgatcacc
     1501 gacgttcgac ccgaatccga tgaagacggt ctccttcatg cgcgtatctc gtgaagtgtt
     1561 attcgtgaat cgtcacgaca aaggagcttt ctgcgaacga cgcttcacgc ttcatcaaca
     1621 acatgatcaa aaacccgcct ttctgattcg ctcaaccgct tccgccaacc gttctttggg
     1681 tgtgcagagc gtcatacgga tgtacccctc tcccggcgcg ccgaatccat tgccgggggt
     1741 tgtcacgata ccggttttct ccaaaaggtg cgcggtaaac gacgtcgacg tataaccctt
     1801 cggcaccgtc acccacacgt agaacgcagc cggaggagga tccacttcta atccgagact
     1861 cttgaggcca ggaataaggg tgtctcgacg agcctggtag ttctttctca agccgtctgt
     1921 catcgaatcg tccagaccta gggcggtaat gccggcttct tgaaccgcct caaaacagcc
     1981 ggagtccaga ttgctcttca ctttgccgag gccggccaag acttccttgt tgcccacagc
     2041 aaagccgatg cgccagccgg tcatattgta ggtcttggag agggaatgga actcaactcc
     2101 gacgtccttt gcgccttcga cttccatgaa gcttgccggg cgacgcccgt catagtacac
     2161 ctctgaatag gcggcatcat ggcacacgat gatctgatgc tcctgagcaa aatccacgac
     2221 ccacttgaag taatccttcg tcatgatcac cgaggtggga ttgttcggcg aattgagcca
     2281 catcagcttg gctttcttag ccacctcttt cgggatcgca ccgagatcag gcaagaaacc
     2341 gatggttttc gtcagcggca tgatgtgcga gat
//
LOCUS       DATMTI010000912         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_247509, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000912 DATMTI010000000
VERSION     DATMTI010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247509"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..420)
                     /locus_tag="VN039_14350"
     CDS             complement(49..420)
                     /locus_tag="VN039_14350"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWV47179.1"
                     /translation="MAILVDTSVWIDHLRTNSPALRRLLDSDLVVCHPLVIGEIACGN
                     MKHRSEVIEYLAVLPTTPIIEYQEVLTFIETHKLFGQGLGWIDVHLLASTMLQHVTLW
                     TLDQSLRNAARRLRCHFESPD"
     gene            complement(423..614)
                     /locus_tag="VN039_14355"
     CDS             complement(423..614)
                     /locus_tag="VN039_14355"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWV47180.1"
                     /translation="MRTTLNIDDHLIKRAVQLTGVQEKTQLVRLGLEALISREAAKRL
                     ARLGGTEPHLKDIRRRRSA"
     gene            complement(763..1896)
                     /locus_tag="VN039_14360"
     CDS             complement(763..1896)
                     /locus_tag="VN039_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV47181.1"
                     /translation="MLRRLRGMLVKEFIQVFRDTRTRTLLFAPPIVQMLIFGYAATLE
                     VRHIPTAVLDYDNSQVSRELLSRFTASLYFDVRAYLNDRREIGDLIDRGEVAVALQIN
                     AGFAQDLRKGQTARLQVIVDSSNSNTALVAVGYINQIAARFARDFQRTQLLRTSPFAP
                     SQMPSIVLERRPLFNVNFDSQWFFVPGVIGNLILVMVVSLTAFAIVREREIGTLEQIM
                     VTPIGRLEFILGKTVPFFLIGLFDTALVSLVGTLWFGVPFRGNIGVLAVGSILFILCM
                     LGIGLFISTISRTQQQAMVSGFFFNMPAITFSGFGTPIGSMPEWLQTLTYLNPLRHFL
                     EVLRGVYLKGVGLDVLWPQMVAMGLLGSIMLAISVARFQKSLD"
     gene            complement(1906..>2274)
                     /locus_tag="VN039_14365"
     CDS             complement(1906..>2274)
                     /locus_tag="VN039_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV47182.1"
                     /translation="PFRGQWTVLFLSCTLFLVVVLSLGYVISVVAKSQLAASQAALIA
                     TFLPAFLLSGFLYPIDQMPTVIQVITHLIPARYFITIIRDVFLKGTSLPFLMDDLLAL
                     ALFATILTVLATRAFHKKLA"
BASE COUNT          586 a          583 c          675 g          430 t
ORIGIN      
        1 atttaagggg tcggatcgga taccttttca atatcaggga gtgatcgctc agtcgggcga
       61 ttcaaaatga cagcgcagtc ttctggcggc gtttcgcaaa gactgatcga gggtccagag
      121 tgttacgtgc tggagcattg tcgaggcgag gagatgtacg tcgatccaac ctaagccctg
      181 tccaaagagc ttatgggttt cgataaacgt caggacttcc tgatattcaa tgatgggcgt
      241 ggtaggcaac acggccagat actcgatcac ttcagaccga tgtttcatgt tgccgcaggc
      301 gatttctcca atgacgagcg ggtgacatac aaccaggtcg ctgtccagca gccgtcggag
      361 agcgggagaa ttggtacgaa ggtggtcgat ccacacggaa gtgtcgacca gtatggccac
      421 gactaggcgg accgacgtcg gcggatgtct ttcagatggg gttcagtgcc gcccaatcga
      481 gcgagccgtt tagcagcttc gcgtgagatg agggcctcga gccccaagcg tacgagctgc
      541 gtcttttcct gcacgccggt gagttgcact gcgcgcttga tgagatggtc gtcaatattg
      601 agtgtggtac gcatgtgcat gaatatgcat ggtcgaagca tatactgtca agcattgcat
      661 tgagtcacca cggtcagaaa agaatagagg gcgtggatca tcctcgtgct aaagatccac
      721 ttcggttagg cgtcaagcca tcagccgcag acaagaggaa gctcaatcca gtgacttctg
      781 aaagcgcgcc acgctgatcg ccaacatgat cgagcctaaa agacccatgg cgaccatctg
      841 cggccacaac acatccagcc ccacgccttt gaggtagacg ccgcggagga cttccagaaa
      901 atgccgcaac gggttgaggt aggtgagagt ttgcagccac tccggcatgc tgccgatggg
      961 ggtgccgaag ccggagaagg tgatggccgg catgttgaag aaaaaccccg acaccatcgc
     1021 ctgctgctga gtcctcgaaa tagttgagat aaataacccg atgcccagca tgcagaggat
     1081 gaacaggata ctgccgactg cgaggacgcc gatgtttccg cggaacggca cgccgaacca
     1141 gagggtgccc accaacgaaa caagggcagt gtcgaacagt ccgatcagaa agaaaggcac
     1201 ggtcttgccg aggatgaact ccagccggcc gatcggtgtg accataatct gttcgagcgt
     1261 gccgatttcc ctctcgcgga ctatggcaaa tgccgtcagg ctcacgacca tgaccaagat
     1321 caggttgccg atcacgccgg gcacgaaaaa ccattgactg tcgaaattga cgttgaacaa
     1381 gggtcgacgc tcgagcacaa tcgaaggcat ctgcgacgga gcgaaagggg atgtccggag
     1441 aagctgcgtt cgttgaaagt cccgggcgaa tcgcgcagcg atctgattga tataaccgac
     1501 cgcaaccaag gcggtattgg aattactgct gtccacgatc acttgcaacc gagccgtttg
     1561 acctttccga agatcctgag caaaacccgc gttgatctga agcgccaccg cgacctctcc
     1621 gcgatcgatc aggtcgccga tctcccgacg atcgttcagg tatgcgcgga catcgaagta
     1681 aaggctggcg gtaaagcgcg ataggagctc tcggcttact tggctattgt cgtaatccag
     1741 caccgccgtg gggatatggc gaacttccaa ggtcgcggca tagccgaaga tgagcatttg
     1801 aacgatggga ggggcaaaca aaagcgtacg agtgcgtgtg tcccgaaaga cttggatgaa
     1861 ttccttgacc agcatcccgc gaagacgtcg cagcatgaag aatgcctatg ccagcttttt
     1921 gtgaaacgcc cgcgtggcca ggaccgtcag gattgtggcg aagagagcca acgcaagcag
     1981 atcgtccatc aaaaaaggaa gcgaggttcc tttcagaaag acatcacgga tgatcgttat
     2041 gaaatagcga gccggaataa gatgggtaat gacttggatc acggtcggca tctggtcgat
     2101 ggggtagaga aaccccgaga gcagaaaggc cggaagaaac gtggcgatca acgccgcttg
     2161 gctggcggcg agttgagatt tggccaccac cgaaatgaca taccccagcg acagaacgac
     2221 gaccagaaat agagtgcagc tgaggaacaa caccgtccac tgcccgcgaa aggg
//
LOCUS       DATMTI010000913         1711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_75957, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000913 DATMTI010000000
VERSION     DATMTI010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75957"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VN039_14370"
     CDS             complement(<1..588)
                     /locus_tag="VN039_14370"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWV47183.1"
                     /translation="MLHKISVIGAGNVGATLVQRLAEAELSKYVTVVDILEGIPQGKA
                     LDIQESAPVYGYDTRVLGSNTYDVIQDSDIVVVTAGIARKPGMSRDDLLATNTKIVTD
                     VCNNIKKFAPKAMVILVSNPLDAMCHVALKATGFPSERVFGMAGVLDSARYKSFIAEA
                     LDVSVEDIQGFVLGGHGDTMVPLPRYTTIAGIPLSD"
     gene            818..>1711
                     /locus_tag="VN039_14375"
     CDS             818..>1711
                     /locus_tag="VN039_14375"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWV47184.1"
                     /translation="MSPSCPTGPLVEINPETLLQTFLEMVRIDSPSGEEEAMRDYLIA
                     RVSEKGLQYQVDDGGNLIVEVPGHQASHEGILVLSGHMDVVPPCHNIQPMVEEIDGDR
                     LISSDNTTVLGADDKSGLAPILEAVFYSMEHQLPRPNIRLIFTTREETALAGAKELND
                     DSLKANFAVTLDHTGKQGVIINQAPTYIEFEIECKGKSVHAGIMPEQGVNAIVFAGKV
                     IEKLHLGRIDELTTCNIGFLTGGKATNIVPDLAVLRGELRGHQSATLQKELAHIEVVL
                     AEVSQSMPGTSYEFKQNTLFEM"
BASE COUNT          369 a          463 c          498 g          381 t
ORIGIN      
        1 atcgctcagc gggataccgg caatggtggt gtagcggggc agcggcacca tggtgtcgcc
       61 gtggccaccc agtacgaagc cctgaatgtc ttccacggac acgtccagcg cttcagcaat
      121 gaagctcttg tagcgggcgg aatccagcac gccagccatg ccgaacacgc gctcgctggg
      181 gaaaccggtg gctttcaggg ccacgtggca catggcgtcc agcggattgg aaaccagaat
      241 gaccattgct ttgggagcga atttcttgat gttgttgcaa acatcggtca cgatcttggt
      301 gttggtggcc agcaagtcgt cacggctcat gccgggcttg cgagcgatac cggcggtcac
      361 gaccacgata tcggaatcct gaatcacgtc gtaggtgttg ctgcccagta cacgggtatc
      421 gtaaccgtaa accggcgcgg attcctgaat atctaaagct ttcccttggg gaatgccttc
      481 cagaatgtcc accacggtga cgtacttgga cagttcggct tcggccaggc gttgcacgag
      541 ggtagcgccc acgttgcccg cgccaatgac ggaaatttta tgtagcatat cgagtttgct
      601 cctttcactc tatcggcctc tatgggttgt atgctaacac aactgtgctt tcctctgtat
      661 ggtttttccg atataaaggg aaggcttgag ggcagttgcc ccaagcccaa tgcgtctggt
      721 gtggcaatcg aggggtatcg tcagaagcaa ccctctctgc ccgatggagc gcaggaatcc
      781 cgtttttccc tgttgtcttg gagaggattt gcccacgatg agcccatcat gtcccactgg
      841 ccccctggtc gagattaacc cggagacttt gctgcaaacc ttcctggaga tggtgcgcat
      901 tgatagcccc agcggggaag aggaagccat gagggactac ctgattgctc gcgtctccga
      961 gaagggcttg cagtatcagg tggatgacgg cggcaacctg atcgtggaag tgccgggcca
     1021 tcaagccagc cacgagggga ttctggtgct gtccgggcat atggatgtgg tgccgccctg
     1081 ccacaatatt cagccgatgg tggaagaaat tgatggggat cgcctcattt cctccgataa
     1141 cacgactgtt ttgggtgccg atgacaagtc cgggctggcc cccattctgg aagcggtgtt
     1201 ctactcgatg gaacaccagt tgccgcgtcc caacatccgc ctgattttta ccacccgcga
     1261 ggaaactgcg ctggccgggg caaaggaatt gaacgatgac agcctgaagg ccaacttcgc
     1321 cgtgacgtta gaccataccg gcaagcaggg cgtgattatc aatcaggctc ccacctacat
     1381 tgagtttgaa attgagtgca agggcaagag tgtccacgcg ggtattatgc cggagcaggg
     1441 cgtaaatgcc attgtctttg cgggcaaagt cattgaaaag cttcacctgg gccgcattga
     1501 tgagctgact acctgcaata tcggtttctt aaccggcggc aaggccacca atatcgtgcc
     1561 ggatctggcc gtcctccgtg gcgagttgcg cggccatcag tcagccaccc ttcagaagga
     1621 actggcccat atcgaggtgg tgctggcgga agtttcccaa tccatgcccg gaaccagcta
     1681 tgagtttaaa caaaacaccc tctttgagat g
//
LOCUS       DATMTI010000914         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_798667, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000914 DATMTI010000000
VERSION     DATMTI010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798667"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="VN039_14380"
     CDS             <1..1542
                     /locus_tag="VN039_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HWV47185.1"
                     /translation="SRLEACLAVLLFFSLASPPPLFSQGPGGQPAKVALDFNEVDIPI
                     FVRFISELTGKNFVLDETIKKQGGKISVFSPTKVLPDQAFNMFVAALEAARLAVVPKG
                     GNLYQVVPMGDLPPERGVFVYKLKHANATDLAAVLTNLVARSQTVAQITPGTRPPIRP
                     LTEFEAPVQVFADKATNSIVISSTKIAWNHLQGVIRDLDIRRKQVFVEAVILEVEVDR
                     LRQIGTDPTQVLGAGKSGFVQGIGGFNRAPEDLATVAQAISGVAAGGATGGAATVLNT
                     LNVRAFMNLLLSLTDTNILSTPQVLAADNQKAKIVVGENRPFPTGQAQGITGGTLVTI
                     ERKDVGVTLELTPQVLEDDLIRLEIKQEITAIETNVAQTIGSGSASIPVGPTTTKRSM
                     ETTAIAQDQQTLVIGGLVRDNITLNESKIPLLGDIPWLGWLFKSQSRNTEKLNLLVFL
                     TPHLVRDESDVVELNARKARDVNSLQRDNRIEEPTRLKQDMLERLEPSPTVPLSPSTE
                     KPNKP"
     gene            1722..2075
                     /locus_tag="VN039_14385"
     CDS             1722..2075
                     /locus_tag="VN039_14385"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV47186.1"
                     /translation="MTDQLVNWRIHRRLPVAYPVIFGGAPFVGEGMVSDLSLSGCAVT
                     CERTVLAGSYIKLSVVLPDPTSSLFIELGKIRWVRENAFGVEFIRVPTLTRHRLDRVV
                     SQELALEANLLPVPA"
     gene            complement(2373..>2966)
                     /locus_tag="VN039_14390"
     CDS             complement(2373..>2966)
                     /locus_tag="VN039_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47187.1"
                     /translation="GGNAGIWFPTRNKLAGFDLGFEITGYIWYPDVNCCKPNFNGTLN
                     ADGSFKGTSTETLGIYVGPNFLIRYPMAICETYPNGRWFPYVGIGVGAHQIAQKPGSS
                     GGVTLGNAIVDSRNTTVGFQGVGGIKGHLWKYVSVFAEAKYLHAHHNGLSTDRFGQSD
                     FFHQQVSGPGPIVNSYSSTIDTILVHAGLSMHFDVKP"
BASE COUNT          703 a          839 c          782 g          642 t
ORIGIN      
        1 tctagacttg aagcctgcct agctgtgtta ctgtttttct ccttggcctc ccctcctcct
       61 ctattctcgc agggaccagg aggccagcct gcaaaggtgg ccttagattt taatgaagtc
      121 gatatcccta tcttcgtccg atttatcagt gagctcacgg ggaaaaactt tgttctggac
      181 gaaaccatta aaaagcaggg tggaaaaatc tccgtgtttt ctccgaccaa ggttttaccg
      241 gaccaggcct tcaatatgtt cgtcgcggcg ttggaagccg cccgcttggc tgtggtgccg
      301 aaagggggga acctctatca ggttgtgccc atgggcgacc ttccaccgga gcggggtgtg
      361 tttgtataca aactcaaaca tgccaatgcg acagatctag ccgccgtgct gaccaatctg
      421 gttgcacgct cacaaaccgt tgcgcagatc acaccaggca ccaggcctcc catcagaccg
      481 ctgacggaat tcgaagctcc ggtccaagtg tttgccgata aggcaaccaa ctccatcgtc
      541 atcagttcga ccaagatcgc ctggaaccat ctccaaggag tgattcgtga cctcgatata
      601 cggcggaagc aggtctttgt ggaggcggtc atcctggagg tggaagtgga ccgcctccgc
      661 caaatcggca ccgatcctac tcaggttctc ggtgcaggaa agtctgggtt tgtgcaagga
      721 ataggagggt tcaacagagc accggaagat cttgcaactg tggctcaggc catcagtggc
      781 gtggccgcgg gcggagcgac cggtggcgca gccaccgttt tgaacacgct caatgttcgc
      841 gccttcatga acttgctgtt gagcctcacg gataccaata ttctctccac accccaggtc
      901 cttgctgcgg ataaccaaaa ggcgaagatc gtcgtcggtg aaaatcgccc gttcccgacc
      961 ggacaagcgc aaggtatcac cggcggaacg ctggtgacga tcgaacggaa agatgttggt
     1021 gtcacattag agttaacgcc tcaagtgctc gaagatgact tgatccgcct tgaaatcaag
     1081 caagagatta cggccatcga gaccaatgtg gctcaaacga tcggctcagg ctcagcgagc
     1141 atcccggtcg gacctaccac tacgaagcga tccatggaaa ccacggcgat tgcgcaagat
     1201 cagcagacgc tcgtgattgg aggactcgtg cgtgacaaca ttacgctcaa cgaaagcaag
     1261 atccctctgt tgggagatat tccctggctc ggatggctgt tcaaatctca gtctcgtaat
     1321 accgagaaac tcaatctcct tgtatttctt actccgcact tggtccgtga cgagagcgac
     1381 gtggttgaat tgaatgctag aaaagctaga gatgtgaatt ctctgcaacg ggacaaccga
     1441 attgaggagc ctaccaggct gaagcaagat atgctcgaac gactcgaacc ctcgccgaca
     1501 gtccctctgt ctccttcgac ggaaaaacct aacaagcctt aagatcggtc tagcgatgaa
     1561 tcgctgcgcc tacgcctcca tgctgaggaa gcggtagctc aatgcgccac tgctccaatc
     1621 cccaaatcgt tatgggatct aggatatgcc ctagcccctc ctccggggaa tggccccttg
     1681 ccatcaagct ggatagactg cgacccacgg aggacatcca catgacagat caactggtga
     1741 actggcgaat acatcggcgg cttcccgtgg catatcctgt gatcttcgga ggcgctccat
     1801 ttgtaggaga agggatggtt tcggaccttt ccctttcagg ctgtgcggtc acctgcgagc
     1861 ggacggtcct tgccggaagc tatatcaaac tgagcgtcgt cttacccgat ccaacatcct
     1921 ctctgttcat cgagttgggg aaaattcgct gggttcgtga aaatgccttt ggcgtggagt
     1981 tcattcgtgt gcccacactt acccggcacc gattggatcg agttgtgtcc caagagctgg
     2041 ccctagaggc gaaccttctc ccagtacccg cctaagctcc actgttatcc atcccaatat
     2101 cccacaggat gttcaaaaag gcattccagc aaggccgcag cgagcgaaga ggcgaggcgt
     2161 actcgttgcc gtacgttaag cctctgagcg atgcgagaac gaagctggat gactttttca
     2221 acattctgcc cgtcatccct cgggatcacc tgatgccagg ccctgcccga tcgatgggaa
     2281 tgaggtgagg agaaggaaga tcggaagcga gaacgtttgg ggcccatcgc accccaccgc
     2341 gtacggtggg ggtatattga tctggcacga ccctacggct tgacgtcaaa gtgcatcgac
     2401 aaacccgcat gcaccaggat cgtgtcgatc gtggatgaat aggaattcac gataggccct
     2461 ggaccgctaa cctgctgatg aaaaaaatcg gactgcccga accgatccgt cgacagcccg
     2521 ttgtgatggg catggagata cttcgcctcc gcaaacacag acacgtattt ccagagatgc
     2581 cccttgatgc ctcccactcc ctgaaacccg accgtcgtat tccgtgaatc gacgatggcg
     2641 ttacccaaag tgactcctcc cgaagaacct ggcttctgcg cgatctgatg cgctcccacg
     2701 ccgatcccga cgtacggaaa ccaccgcccg ttgggatagg tctcgcaaat cgccatggga
     2761 tagcgaatca agaaattggg gccgacgtag atgccgaggg tttcagtcga agtaccctta
     2821 aacgatccgt ctgcattaag cgtcccatta aagttaggct tacagcagtt cacgtccgga
     2881 taccagatat agccggtgat ttcaaaaccg agatcgaacc ccgccaactt gtttctggtg
     2941 ggaaaccaga tccccgcgtt gccccc
//
LOCUS       DATMTI010000915         1735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_670470, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000915 DATMTI010000000
VERSION     DATMTI010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670470"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VN039_14395"
     CDS             <1..1494
                     /locus_tag="VN039_14395"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HWV47188.1"
                     /translation="VDGKFSVPAPAGDHILVHIEANGFAEAFPVAQVRSGQSTSLGVK
                     LVPIGATATISVAEGDTVSVPNSPARVTIPANGLVPRAGRMPAETVTVSLTSINPAID
                     PNLMPGGFEGISVGGGAAQPIESFGAMLVDIKDRAGTRYTLAEGKTSTIRIPLGTQSA
                     DPPATVSLWFLDETAGVWKEEGTATLQGTAPNQYYEGTVAHFSYWNADITTEQIFITG
                     CVRDGNGQPVLNALVRTEGIDYTGVAKHFTTADGTFQVAMRKNGRAKLGLSEFDQQTF
                     NFVLISNTVSVGPSATDIHLPNCLVKKPDPLAIMTVVLTGGNVGSAYNQTLAADGGVP
                     GYIWSLNTGSNPLPVGLTLTETGIIAGTPTTAGITTIIVKVTDSAEATVTRKFTLTIN
                     APGVQPLAITTATLPAGTVGTSYDATLTSTGGTGSKSWSVSYGALPIGLSLDAASGVI
                     SGTPTRAGVFFCTIRVQDGGTPPQSNEISFDTMINPAYEGEGSLGAP"
     gene            1491..>1735
                     /locus_tag="VN039_14400"
     CDS             1491..>1735
                     /locus_tag="VN039_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47189.1"
                     /translation="MTDAPASVGRTFLADESLTTMRFAGFTGEGISWNETRNAGTRIK
                     TITVLFDLAGHVSTLGFFYIDPEGGGRWGCEPAAGGCS"
BASE COUNT          381 a          462 c          532 g          360 t
ORIGIN      
        1 gtcgatggga agtttagcgt cccggcgcct gccggagatc atatccttgt tcatatagaa
       61 gcgaacggat tcgccgaggc gtttccggtc gcacaggtca gatcgggaca atcgaccagt
      121 ctcggtgtga agcttgtgcc gatcggcgcg acggcaacga tctccgtggc ggagggcgat
      181 actgtgtcgg tgcctaactc accggcccgg gtgacgattc ccgccaatgg tttggtgcca
      241 cgggcgggaa ggatgccggc tgagacggtc actgtttcgc tgacctcgat caatccagcc
      301 atagacccga atttgatgcc gggcgggttc gaggggatct ctgtcggcgg tggggcggcg
      361 cagccgatcg aaagcttcgg agcgatgtta gttgacatta aagatcgtgc cggaactcgc
      421 tacacattgg ctgaaggaaa gacctcgacg attcgtattc cactcgggac gcagtccgct
      481 gatcctccgg ccacagtctc gctctggttc ttggatgaga cggcaggggt gtggaaggaa
      541 gaggggaccg cgacgttaca aggcacggct ccgaaccagt attacgaagg aacggtcgct
      601 cacttcagct attggaacgc cgacatcacc actgaacaga tcttcatcac cggctgtgtg
      661 agggatggca atgggcagcc ggtactcaat gctctcgttc gaacggaagg tatcgattat
      721 acaggcgtgg cgaaacattt cacgactgcc gatggaacat tccaggttgc catgcgcaag
      781 aacggccggg cgaagctcgg tctctctgaa ttcgatcaac agaccttcaa ttttgtgctc
      841 atctcaaata cggtgagtgt cggaccatcg gcaacagaca ttcatctacc caattgcctc
      901 gtgaagaagc cggacccact ggcgatcatg acggtagttt tgaccggagg caatgtcgga
      961 tcggcgtaca atcagacttt agctgcggac ggcggggtgc ctggctatat atggagtctg
     1021 aacaccgggt caaatccttt gccggttggg ttaacgctaa ctgagaccgg catcattgcc
     1081 ggtactccaa cgacggctgg catcacgacg atcatcgtca aggtgaccga ctcagcggag
     1141 gcgaccgtga cgagaaagtt cacattgacc atcaacgcac cgggggtaca acctctggcg
     1201 atcacgacgg caacacttcc tgccgggaca gtgggcacat cgtacgacgc cacattgacg
     1261 tcgacggggg gcaccggctc caagtcttgg agcgtcagtt atggggcctt gccgatagga
     1321 ttgtcgctgg acgcggccag cggcgtcatc agcggcacgc cgactcgggc cggagtgttc
     1381 ttctgcacga ttcgcgttca agatggcggt acgccgcctc aatcgaacga gatctcattc
     1441 gacacgatga tcaaccctgc atatgagggc gaagggagct tgggagcacc gtgaccgatg
     1501 ctccagccag tgtcggtcga acattcctgg ccgatgaaag ccttaccact atgagatttg
     1561 ccggctttac cggcgaaggg attagttgga acgaaactcg caatgctggg actcgtatca
     1621 aaacaatcac cgtgttattt gatctcgcgg gtcatgttag caccttggga ttcttctata
     1681 tagatccgga aggaggaggg cgatgggggt gcgagcctgc cgcaggcggc tgttc
//
LOCUS       DATMTI010000916         5640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_374270, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000916 DATMTI010000000
VERSION     DATMTI010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374270"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..928)
                     /gene="meaB"
                     /locus_tag="VN039_14405"
     CDS             complement(143..928)
                     /gene="meaB"
                     /locus_tag="VN039_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWV47190.1"
                     /translation="MSPLGSHPFLCDVHGLMTLAEQVRAGNVRAISRLISLLENHPGA
                     REALRLLNSTSRGTRVIGITGYPGAGKSTVVNCLVTFYRRQGLKVGVLAVDISSSVTG
                     GALLGDRIRMQEHALDHGVYIRSMATRGHHGGLAQATGDAALVLEAAGYDVILIETIG
                     VGQNEVDIVDLVHIVVAVVAPGLGDEVQAMKAGLLEVAHIIVVNKGDLPGADSTLRDL
                     REWCPNVLRTVATTGEGIPELAAAISECYQSRSGSHIGEARSC"
     gene            complement(925..1872)
                     /locus_tag="VN039_14410"
     CDS             complement(925..1872)
                     /locus_tag="VN039_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HWV47191.1"
                     /translation="MIGDSNGPTSHASVIRIVEVGPRDGLQNESNVVPTAVKVSFVDA
                     LSKSGVAEIEAGSFVSPRAVPQLADSDEVFRRIERLPGVIYSALVPNERGLERARAAA
                     VTKIAVFTAASDSFTRRNINCTIGESIERFKPVVFGAKRDGMTVRGYVSTVTHCPFEG
                     AVQPAQVLDVVRQLLDLGVDEISLGDTVGKAAPRDIRMLLDEIVPRIDRSRLSLHFHD
                     TCGMAVANVLTAWTEYRIEAFDTAAGGLGGCPYAPGASGNVSTEDVVYALKASGASLI
                     VDERKVIAAASEIGKLLNCRLSSRLSQVQTEQTAYEGAA"
     gene            complement(1896..2696)
                     /locus_tag="VN039_14415"
     CDS             complement(1896..2696)
                     /locus_tag="VN039_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV47192.1"
                     /translation="MMHPTSILVESSKGYARVTLNRPDRRNAFDACMVEELCEIFKTL
                     GQDHSVRAIVLTGRGPTFCAGVDLRWMGTGRSLSTSEARRDAEQLLAMFRTIDNCPCP
                     VVGCVQGSVYGGGIGLVAVCDIAVAAASATFAFSEVRLGLVPAVIAPFVLAKVGDSFV
                     RRFCLTGEAFPASTAQGNGLIHDVVEPAALEAHIASLTERIIHLAPQAVRDTKALFRR
                     LHHLSREECWKACVEGNIRARLSSEAQEGIRAFRERRTPNWVMPTGGE"
     gene            complement(2704..4308)
                     /locus_tag="VN039_14420"
     CDS             complement(2704..4308)
                     /locus_tag="VN039_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWV47193.1"
                     /translation="MRTLTTSVVAASGEFASNRAHYEGLVADLQKHLTLARSGGSPEA
                     TALHQQRGKLTARERIAKLLDPNAPWLELSPLAASDMYDDHIAAAGLITAIGFVAGRP
                     CVIAANDATVKGGTYFPMTIKKHLRAQEIALENRLPAVYLVDSGGVFLPMQADVFADK
                     EHFGRIFYNQARLSALGVPQIAVVMGMCTAGGAYVPAMCDENVIVKGTGTIYLAGPPL
                     VKAATGEEVTAEELGGADLHTRLSGVSDHLADDDHEALEICRSIVETLPQRPVLRRPA
                     PIEEPQYPASELYGLIPNNPRQTFDAHEVIARLVDGSRFHEFKARYGRTLICGFARWM
                     GHQVGIVANNGVLFSEAALKGAHFVQLCAQRRLPLVFLQNITGFMVGKEYETRGIIKD
                     GAKMVQAVATADVPKFTIIIGASHGAGNYAMCGRAYAPRFLFLWPNARTSVMGAQQAA
                     QVLLTVKRQQRTRDKASLSEDEQRKITDSIRTQYEREGSPYFSTARLWDDGIIDPVDT
                     RNILGLCLDVAMTTPVGSSRFPVFRM"
     gene            complement(4315..4722)
                     /locus_tag="VN039_14425"
     CDS             complement(4315..4722)
                     /locus_tag="VN039_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWV47194.1"
                     /translation="MTVASAPLRILIGKVGLDGHDRGVKLIARALRDAGVEVIYTGLH
                     QMPEQVVNTAIQEDVDAIGLSILSGAHKTLFRRVLELLKEQDAGDIALFGGGIIPDED
                     VAALTADGVKALFRPGTPFQEIVTFAKGLKPQG"
     gene            complement(4719..5558)
                     /locus_tag="VN039_14430"
     CDS             complement(4719..5558)
                     /locus_tag="VN039_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV47195.1"
                     /translation="MKLGAFEIYPVSDGRFRLDGGAMFGVVPKVLWEKCCPADDLNRI
                     SLSLTALLIRANGKNILVDTGVGPKEEEKFRNMFAVERKPTILESLKRLGLGPEDIHL
                     VINTHLHFDHAGGNTMQENGAVVPTFPNAKYFVQHGEFEDAARANERTKASYRRDNFT
                     PIDHINQWEFLHGDTELVPGVTTVVTAGHTRCHQSVKIESEGQVAFFLGDLIPTVSHL
                     PLPYIMGYDLFPIQTLETKRWVLDRAFEKRWLLLFEHDPVVQAGYVRRDQDGKYFLHE
                     VAA"
BASE COUNT         1182 a         1766 c         1502 g         1190 t
ORIGIN      
        1 ctagcagttc catggtcgcg cccggagaaa ctcctcaatt tgggttgtct ttccttcgcc
       61 ctctcttcat tgtcgccgcc tgataccccc cttcgacttt cccctttccg ttcctcactt
      121 catccttgcg gttcgaacgg agctagcagc tccgagcttc accgatatgg ctgccactac
      181 gggactggta acactccgag atcgccgcag cgagctctgg aatcccttca cctgtcgtcg
      241 cgaccgtccg taacacattc ggacaccatt cccgcagatc tcgtaacgtg ctatccgcgc
      301 cgggtaggtc tcccttgttg acgacgataa tgtgtgcgac ttccaatagt ccggccttca
      361 tagcttgaac ctcgtcgccc aggccaggcg ccaccactgc cacaacgata tgcacgagat
      421 cgacgatgtc gacttcattc tggccgactc cgatggtctc aatcaggatc acatcatacc
      481 cggctgcctc caatactaac gctgcatcgc cggtcgcttg ggcaagccct ccatgatgtc
      541 cacgggtggc catgcttctg atgtacaccc catgatccag cgcgtgttcc tgcattctga
      601 tacggtcacc caacagagca ccgcctgtga cggagctact gatatcgacc gccaggaccc
      661 ccactttcaa accttgccgt cgatagaaag tgactaagca gttgaccacc gtgctcttcc
      721 cggctccggg atatcccgtt atcccgatga ccctcgtccc ccgcgaggtg ctgttgagga
      781 ggcgcagcgc ttctctggca cccggatgat tttccaacaa ggagatcagg cgtgatatgg
      841 cgcggacgtt tccggctcta acctgttcag ctaaggtcat caagccgtgg acgtcgcaca
      901 ggaacgggtg cgaaccaagg ggactcatgc cgccccctca tatgcggttt gttctgtctg
      961 tacttgcgag aggcgcgacg acagtcggca gttcagaagc ttcccgatct cgcttgccgc
     1021 cgcgatgacc ttccgttcat ctacgataag cgaagcgccc gatgccttca gcgcatagac
     1081 cacgtcttca gtcgaaacgt tccccgatgc gcccggtgca tatggacacc ctccgagtcc
     1141 cccggcagcc gtgtcaaacg cctcaatgcg gtactccgtc catgcggtca ggacgttggc
     1201 cacagccatg ccgcaggtat catgaaagtg gagggagaga cgacttcggt caatgcgggg
     1261 cactatttca tccaacagca tccggatgtc tcgaggggcg gccttcccaa cggtgtcccc
     1321 gagagagatt tcgtctacgc cgagatcaag taactgccgc accacgtcca gaacttgcgc
     1381 cggctggacg gcaccttcaa agggacaatg ggtcacggta gaaacatatc ctcgcacggt
     1441 cataccatcg cgtttcgcac cgaacaccac cggcttgaac cgttcgatcg actccccaat
     1501 cgtgcagttg atgttccgtc tggtgaacga atcggaggct gccgtaaaga cagcgatctt
     1561 ggtgactgcc gccgcccttg cccgttccaa cccacgttcg ttcggcacca gcgctgaata
     1621 gataacgccg ggcagtcgct cgatcctccg aaacacctcg tcggagtccg ccagttgcgg
     1681 aacggcacga ggcgacacaa acgatcctgc ttcgatctcg gccacaccgc tcttggacag
     1741 ggcatccacg aacgacacct tgaccgccgt ggggacgaca ttcgattcat tctgcaaccc
     1801 gtctcgcgga ccgacctcga cgatccggat cacagacgca tgtgatgttg gtccgttgct
     1861 gtcccctatc atgcaacctt ccacagcagc ctcttttact ccccgcctgt cggcatgacc
     1921 cagttcgggg ttcggcgttc gcgaaaggca cgaatcccct cctgcgcctc agatgaaaga
     1981 cgagctcgaa tgttcccttc gacacaggct ttccagcatt cctctcggga caaatggtga
     2041 agtcggcgaa acagcgcttt cgtgtcccgt acggcctgcg gagcgagatg gatgatccgt
     2101 tcagtcaatg atgcgatatg ggcctctaac gcagccggtt cgactacatc atggatcaga
     2161 ccgttgccct gagcggtgga tgcgggaaat gcttcaccgg ttagacagaa tcgtcgcaca
     2221 aaagagtccc cgaccttcgc aagaacaaag ggcgcgatca cagcggggac caatcccaag
     2281 cggacctcgc tgaacgcgaa tgttgcactt gcagcggcta cggcaatgtc gcacactgca
     2341 accaacccga ttcctccgcc atagacggac ccctgaacgc atccgaccac gggacagggg
     2401 cagttatcga tggttcggaa catcgcgagc aattgctcgg catcccgccg cgcttctgat
     2461 gtggacaggc tccggccagt ccccatccag cggagatcta ccccggcaca aaatgtcggg
     2521 cctctaccgg tcaagacgat tgcacgcacc gagtgatcct gtcccaatgt cttaaagatc
     2581 tcgcacagtt cctccaccat gcaggcatcg aacgcgttcc gccgatcggg ccgatttaag
     2641 gtcacacgtg cgtatccctt actcgactca acgaggatcg acgtaggatg catcataggc
     2701 gcctcacatg cgaaaaacgg ggaagcgcga cgagccgacc ggcgtcgtca ttgcgacgtc
     2761 aagacacaga ccgagaatgt tccgtgtatc cactggatcg atgatcccat catcccagag
     2821 ccgtgccgtg ctgaaatagg gactcccctc ccgctcatac tgtgtgcgaa tggagtccgt
     2881 gatctttcgc tgctcatctt ccgacaggga cgctttgtcg cgggtccgct gctgccgttt
     2941 caccgtcaag agtacctggg ccgcctgctg agcccccatc accgacgtcc gcgcgttggg
     3001 ccagagaaaa agaaagcgcg gagcataggc ccgtccgcac atggcgtaat taccggcacc
     3061 gtgagaggcg cctataataa tggtgaattt cgggacatcg gcagtcgcca ccgcttgcac
     3121 catcttggct ccatccttga tgatccctcg tgtctcatac tccttaccga ccatgaagcc
     3181 tgtgatgttt tggagaaaca ccagcggcag ccgccgctga gcacagagtt ggacaaagtg
     3241 ggcgcctttc aaggcagcct cggagaacag cacgccgttg ttcgcgacga tgccgacttg
     3301 gtgccccatc caacgggcga agccgcatat gagcgtccgt ccgtagcggg ccttaaactc
     3361 atgaaagcgg ctgccgtcca ccaagcgcgc aatgacttca tgggcgtcga aagtctgccg
     3421 tggattgttc ggaatgagtc catacaactc agaagccggg tactgcggct cttcgatcgg
     3481 tgccggtctg cgaaggaccg gccgttgagg caatgtctcg acgatcgacc ggcagatctc
     3541 gagcgcttca tggtcatcgt ctgcaaggtg gtcgctcaca cctgagagcc tggtgtggag
     3601 atcggccccg ccgagttctt cagccgtgac ctcttcgccg gttgccgctt tgaccagtgg
     3661 cggccccgcg agataaatgg tgccggtccc tttgacgatc acgttctcat cgcacatggc
     3721 cggcacgtaa gcaccaccag cagtgcacat ccccatgacg acggcgattt gcggaacccc
     3781 aagagcagac aggcgcgcct gattgtagaa gatccgtccg aagtgctcct tgtcggcaaa
     3841 gacatcggcc tgcatcggca gaaacacgcc gcctgaatcc acgagataga ctgcgggcaa
     3901 tcgattctcc aacgcaatct cctgtgcccg gagatgtttc ttgatcgtca tggggaagta
     3961 ggtcccgccc ttgaccgtcg cgtcattggc ggcgatgaca caaggtcgtc ccgccacgaa
     4021 accgatggcg gtaatcagac cggcagcagc aatatggtcg tcgtacatgt ccgatgccgc
     4081 caaggggctg agttccagcc aaggtgcatt tggatcgagc agtttcgcaa tccgctcccg
     4141 tgcggtcagt ttgccacgtt gttgatgaag ggcggttgct tccggtgacc ctccggatcg
     4201 agcgagggtg agatgtttct ggagatccgc caccagaccc tcatagtgcg cacggttcga
     4261 cgcgaattcg ccggaggcgg caaccactga agtcgtcaac gtacgcatgg tttttcagcc
     4321 ctgtggtttg agccctttgg cgaacgtcac gatttcctgg aaaggcgtgc caggtctgaa
     4381 gagagccttc acgccgtccg ctgtgagcgc cgcgacatcc tcatcgggaa tgattccacc
     4441 gccgaacaag gcgatgtctc ctgcatcctg ttccttgagg agttccagga cgcgtcggaa
     4501 cagcgtctta tgcgctcctg aaaggatgct caagccgatc gcatccacat cctcttggat
     4561 cgccgtattg acgacctgtt ccggcatctg atgcagaccg gtatagatga cttccacccc
     4621 ggcgtctcgt aatgctcgcg cgatgagctt cacacctcgg tcatggccgt cgagaccgac
     4681 tttgccgatc aaaatccgca gcggagcgct cgctactgtc atgccgccac ctcatgaaga
     4741 aaatatttgc cgtcctgatc cctccgaacg tatcctgcct gaaccactgg atcatgctca
     4801 aagagcagca gccatcgctt ttcgaacgcc cgatccaaca cccatcgttt cgtctccaag
     4861 gtctggatgg ggaagagatc gtagcccatg atgtaaggaa gcggcagatg cgacacggtc
     4921 ggaatcaggt cgccgaggaa aaacgccacc tgtccttcag attctatctt gacgctttga
     4981 tggcagcgcg tatggccggc ggtgacgacc gtggtcacac ctggaacaag ttccgtgtcg
     5041 ccatgcagga attcccattg attgatgtga tcgatcggtg tgaaattatc tcgccggtag
     5101 ctggctttgg tccgttcatt ggcgcgagcc gcatcttcaa actcgccatg ttgaacgaaa
     5161 tatttggcat ttgggaaagt gggtacgacg gccccgttct cttgcatcgt attcccgcca
     5221 gcatggtcga aatgcaggtg cgtattgatc accagatgga tgtcctccgg tccaagacct
     5281 aaccgcttca acgattcaag gatggtcggt ttccgctcga cggcgaacat attccgaaac
     5341 ttctcttctt ccttaggacc caccccggta tccaccagga tattttttcc gttggctcga
     5401 atcagcaaag cggtgaggct gagggagatt ctgttcaagt catcggcggg gcaacacttc
     5461 tcccacagga ccttcggcac aacaccgaac atagcacccc catccagccg aaaccgcccg
     5521 tcactcacgg gataaatttc aaatgccccg agtttcataa ctctctctcc ccctaagccg
     5581 cggttgaaag ggtatgggat accgagcggg cgccatatcc tctctctcat cctcttcggc
//
LOCUS       DATMTI010000917         3810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_544123, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000917 DATMTI010000000
VERSION     DATMTI010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544123"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            596..1021
                     /locus_tag="VN039_14435"
     CDS             596..1021
                     /locus_tag="VN039_14435"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWV47196.1"
                     /translation="MIMSMQSLPTRDARPLSIEQVVSLAQQVFPTGELRYIGVPYEDA
                     DIYHLALRQIGEVGESNGQSQIWLDQYSGKVLRVHDWNRFTGGETFLAWLFPLHNGEA
                     FGLTGRWIVFIAGFVPLLLYITALRMWWLKRQAHRRQLA"
     gene            1202..1447
                     /locus_tag="VN039_14440"
     CDS             1202..1447
                     /locus_tag="VN039_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47197.1"
                     /translation="MTLTYAHIEELFHRYRAELTRRLLAMVSSRDAAADLLQDTAVLY
                     HLTKTFPTADSRSRRSPHRPLLMYPRNFFLCRPSGEV"
     gene            1506..>3810
                     /locus_tag="VN039_14445"
     CDS             1506..>3810
                     /locus_tag="VN039_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HWV47198.1"
                     /translation="MRTSRKDANERRGVEEFIIVWACMLLMFIGSLVDTSAAQEKSAS
                     MVDFNIPAQPLGSVLNAFAVTSGWQVSAASDLTTGVDSPGAIGRYSPEQAIQAILAGT
                     GRNYRVDGVQMITIERVSSAPAAVESVSEGPAAQPPAQKSGKLVKVPEIVVKDVRHGD
                     RDTMSYAAERANTATRTDTPLIQVPQSVEVVTQKVIQDQRAVRVEQALRNVSGVALGD
                     VGQSGIAADVAYCRGFPCGYFNNYLRNEDQNQALTFRDISNIQRLEVLKGPASVLYGR
                     SQPGGIVNILTKQPQAERYASIDQIIGNYNYYRTMIDVTGPLNESKTLLFRINGAYEN
                     TESFRDIVRGQRYFVAPVFTWLVGNNTTITIEGEYIRDRRTPDFGIPAIGRGLASLPD
                     SRFLGERFDTLAFEEARAGLTVEHRFNSNWRIESRFRADRSTATAYRTVAGGLLPDNQ
                     TMARLIFDQLAPISSYYWRNDVIGKFSTGSIGHNLLAGVEIGRQYASYDQAAALFSAI
                     NIFNPVYGASVPALNRTPFSHSFANALGWYAQDQISLLDNFHILIGARGDYFYQHSNV
                     AGADTKAEDFAFSPRVGVTYLPIPQLSLYANFTRSFIPNFGPFTAARDQFDPSMGTQY
                     EAGVKVDIVPGRLTSTVAVYRITKTNVLAVDPTNSLFFVQTGEQRSQGVEFDVTAQLT
                     PGWRVIATYAYTDARITADTTVSIGNRLPLVARHTGSFWTTYDLQNGPLKGFGAGIGF
                     FAVGGRAGDLANSFGLPGYVRTDAALYY"
BASE COUNT          796 a         1051 c         1117 g          846 t
ORIGIN      
        1 atccattctg aatctgcttc gagtcaactt gaaggaagtg cgcatgtctt tttattgtcc
       61 tcgagatatc tccttgccat gtcgaacctc cggcaggttc atgaacactc atgcgccata
      121 tcccccgtgg atgggattca atcgttctca atgtggccaa cgaggcatac cgtgcgcgag
      181 caaggtagaa ccatgagtga atctcgaacg ccggccggat cgggtgtgga tgtccggtgc
      241 cactgcggga agctgatggc tcgctggcaa ggccagagct tggtcatcaa atgtgcgcgc
      301 tgtaggcgat tcattaggat tcacgcgtcc gccatcggcg gtaggccacc tgaattgtaa
      361 cttggtttac atcggtagct cctgttgtag tcagtccacc cctaggctgt ggttcctcga
      421 agtcggcatg tactgcctgg aggaggcggc gaggatagcc ctgcgaatcc gtgcatctga
      481 ggtccgcgga gcaaagagcc gggtgagttt tctgcttgct ttcccgtctt aagtggatag
      541 gtgaggctgt tcacacgcag gccaagttcc tcgctagatc cagtggacga ccgtcatgat
      601 tatgtccatg cagtcgcttc cgactcgtga tgctcggcca ttgtccattg agcaagttgt
      661 ctcccttgct cagcaagtct tcccgacagg ggagttgcgg tacatcggcg taccgtacga
      721 ggacgccgac atctatcatc tcgctctgcg tcagataggt gaggtgggcg agtcgaatgg
      781 tcaaagccag atatggctgg atcaatacag cggcaaagtg cttcgagttc acgattggaa
      841 tcggttcacc ggcggtgaga cctttctggc ctggctcttc cccctgcata acggcgaagc
      901 gttcggcctc accggacgat ggatcgtttt catcgccgga tttgttccgc tcctgctgta
      961 catcaccgcg ctgcgcatgt ggtggctgaa acggcaggct caccggagac agctggcctg
     1021 atttccatca ggctctgaca gaggctggac tttgacgtta ttctcggtcg cgctgaggcc
     1081 ggcgttcttc aggaacagcg cccacggcat tgtgagatgc acggggggca ttttgtgccg
     1141 ggatgccgaa tcgcgtgaga tgaatggagt gtgagccgat gacaatggaa cacagggacc
     1201 gatgacgctt acttatgccc atatcgaaga gctgttccac cggtaccggg cagaactgac
     1261 gcgccgtttg ctcgccatgg tgagttcacg cgacgcagca gccgatttgt tgcaagatac
     1321 cgccgtgctc taccacctga cgaaaacgtt tccgactgca gacagtcgct ctcgacgatc
     1381 gcctcatcgt cctcttttaa tgtatccccg gaattttttt ctttgccgcc cgtccggtga
     1441 ggtgtgaacc gcctcgctct atccgtctac tcatacatgg catggatgga atacggagac
     1501 gggtgatgcg tacgagcagg aaggatgcaa acgagcggag aggggtcgag gaattcatca
     1561 tagtatgggc ctgcatgctc ttgatgttca tagggtcgtt agttgacacc agcgcggcgc
     1621 aagaaaaatc ggccagtatg gtcgacttca atatcccggc gcagccattg ggatcggtct
     1681 tgaatgcctt tgccgtcact agcggttggc aggtaagcgc ggcaagcgac ctgacgacag
     1741 gtgttgactc tcccggtgcg atcggacgct acagccccga acaggcgata caggcgattt
     1801 tggccggaac gggtcgcaac tatcgcgttg atggcgtcca gatgatcacg atcgagcgtg
     1861 tctcctccgc tccggcggca gtcgaatcgg tctcggaagg gcctgctgca cagccgcctg
     1921 cccagaaaag cgggaagctg gtcaaagtgc cggagattgt ggtcaaagac gtgcgccatg
     1981 gtgaccggga cacgatgtcg tacgctgccg aaagggcaaa tacagccacg cgcaccgaca
     2041 cgccgttgat tcaagtgccg caatcggttg aagtcgtgac gcagaaagtc atccaggatc
     2101 agcgggccgt tcgcgtcgaa caggcgctac gcaatgtcag cggcgtggcc ttgggcgatg
     2161 tcgggcaatc cggtatcgcc gccgatgtgg cctattgtcg cggattccct tgcggatatt
     2221 ttaataacta tctgcgcaac gaagaccaga atcaggcgct cacttttcgg gacatctcca
     2281 atattcagcg tctcgaggta ctcaaagggc ctgcatcggt cctctacggc cgaagccagc
     2341 cagggggcat cgtcaacatc ctgacgaagc agccgcaggc ggaacggtat gcctccattg
     2401 atcaaatcat cggcaactac aattattatc ggaccatgat cgatgtcacc ggtccactga
     2461 acgagagcaa gacgctgctc ttccgtatca acggagccta tgagaacacg gagagttttc
     2521 gggatatcgt gaggggacag cggtatttcg tggcacccgt cttcacttgg cttgtcggca
     2581 acaacacgac cattacaatc gagggagaat acatccgaga tcgccggacc cctgacttcg
     2641 gcattccagc gatcggacgg ggactggctt ccttgcctga cagccgtttc cttggcgagc
     2701 ggttcgatac cttggcgttc gaggaggctc gagccgggct cactgtcgaa catcgcttca
     2761 acagcaattg gcgtatcgag agtcggttta gagcggatag atcgacagcg acggcctatc
     2821 gaaccgttgc gggggggcta ttgccggaca accagacaat ggcgcggttg atcttcgatc
     2881 aactcgctcc catctccagt tactactggc ggaacgatgt gatcggcaaa ttttcaaccg
     2941 ggtccatcgg gcacaatcta ttggccgggg tcgaaattgg gaggcagtac gcatcgtacg
     3001 atcaagccgc ggctctgttc agtgcgatca acattttcaa tccggtctat ggcgcatccg
     3061 taccggcgct gaatcgaaca ccgttcagcc atagtttcgc gaatgcgctc ggctggtatg
     3121 cgcaagacca gatctcgctg ctcgataact ttcacatcct gatcggcgcc cgcggcgatt
     3181 atttctatca acacagcaat gttgcggggg cagacacgaa agcggaagat tttgcattca
     3241 gcccgcgtgt cggcgtgacc tatctcccga ttccgcaact ctcgttatat gcgaacttta
     3301 ctcggtcctt tattccgaac ttcggtccgt ttaccgccgc gcgggatcaa ttcgacccgt
     3361 ccatgggcac gcaatatgaa gccggggtca aggtcgatat cgtaccgggc cgattgacct
     3421 cgaccgtggc cgtttaccgg atcacgaaga cgaacgtgct cgcggtagac ccgaccaatt
     3481 cgctattttt cgttcagaca ggcgagcaac gtagccaagg cgtcgagttc gacgtgacgg
     3541 cgcagctgac gccggggtgg agggtcattg cgacctatgc ctatacggat gcgcgcatca
     3601 cggcggacac gaccgtttcg atcggtaatc ggcttccgct cgttgcgaga cacaccggaa
     3661 gcttctggac gacctatgac ctgcaaaacg gcccgttgaa aggattcggg gccggcatcg
     3721 gcttctttgc cgtgggaggg cgggccggag accttgccaa ctcattcgga ctgccgggtt
     3781 atgtgcggac ggacgccgca ctgtactatc
//
LOCUS       DATMTI010000918         1620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_755750, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000918 DATMTI010000000
VERSION     DATMTI010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755750"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VN039_14450"
     CDS             complement(<1..1322)
                     /locus_tag="VN039_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWV47199.1"
                     /translation="MTSHPERSTSAGTALAALGVVYGDIGTSPLYALRECFHISHGLS
                     VTLPTITGLLSLIIWALLLVVTVKYLLFVMRADNQGEGGILALMALGQRHREESAFPL
                     RIGPVIALGLLGASLVYGDGIITPAISVLSAVEGIEVETTAYQPYTLPIAIAVLLAFF
                     AIQSRGTARLGEWFGPIMLLWFVTLAALGIKSAARTPEVFAAVSPHHAVQFLIEHPAR
                     GFAVLGSVFLVLTGAEALYADMGHFGKAPIRLGWYSVVLPSLVLQYLGQGALLIRQPE
                     AVTNPFYLLAPGWFLLPLVILATMATIIASQAMLSGAFSLTHQAIQLGYLPRMDIRYT
                     SASHIGQIYVPAMNVLMLIGTVGLVVLFGTSSNLAAAYGIAVAGTMVLSTLLISVVAR
                     RQWKWNLPAVVLVTSFFLIMDLSFFGANVLKIPHGGWLPLVLGAGLFL"
BASE COUNT          403 a          446 c          495 g          276 t
ORIGIN      
        1 aaaagaaata aaccggcacc gagtactagc ggaagccaac cgccgtgagg gatcttgagc
       61 acattggccc caaaaaacga gaggtccatg atcaggaaaa aactagtgac gaggaccacg
      121 gccggcaggt tccatttcca ttggcgccgg gcaacgacgg aaatcaagag tgtggaaagg
      181 accatggtgc cggccacggc gatcccatag gcagcggcca gattgctgga ggtgccaaag
      241 agcacgacca gcccgacagt accgatcagc atgagtacat tcatggccgg cacatagatc
      301 tgtccgatat gagaagcaga cgtgtatcga atgtccatgc ggggcagata gcccaattga
      361 atcgcctgat gggtcaggga aaaggcaccg ctcagcatcg cttgagaggc gatgatggtg
      421 gccattgtcg caagtatgac caacggcagg agaaaccatc cgggtgccag caagtaaaac
      481 ggattcgtca ccgcctccgg ctgtcggatc agtaaggcgc cctgtcccaa gtattgcagc
      541 acaagcgacg gcaacaccac gctgtaccag cctaatcgaa tgggcgcttt cccgaagtgc
      601 cccatgtcgg catacaaggc ttcggctcca gtaaggacga gaaacacgct ccccaatacc
      661 gcaaagcctc gcgcgggatg ctcaatcaag aactgaacgg cgtggtgggg gctgacagcg
      721 gcaaacacct ccggagtccg tgcggcactc ttgatcccca aggcggcaag cgtgacgaac
      781 cagagcagca tgataggacc gaaccattcg ccgagtcgtg ctgttccgcg cgactggatc
      841 gcaaagaacg ccagcaagac tgcgatggcg atcggcaacg tatagggttg ataggcggtc
      901 gtctcgacct cgatcccttc cacggcgctc aagaccgaga tggccggcgt aatgatcccg
      961 tccccataga ccaacgaagc cccgagcagg ccgagcgcga tcactgggcc gatccgcaag
     1021 gggaacgcgg actcttcccg atggcgttgt cccaaggcca tcaacgccaa aattcctccc
     1081 tcgccttgat tgtctgctcg catgacgaac agtaagtact tcaccgtgac gaccagcagc
     1141 agggcccaga tgatgagcga gagtaggccg gtgatcgttg gaagggtgac ggacagaccg
     1201 tgagagatgt gaaagcattc gcgcagcgca tagaggggac tggttccgat atcgccgtac
     1261 acgacaccaa gcgcagccag cgccgttccc gcggacgtcg agcgctcggg gtggctagtc
     1321 atcggtgcga cggaacggcc agaggagtag atctagatga gtaagagtca aaccgtagat
     1381 actaggcata cggcatcgat cctagccggt tcaggaagtc agaacaaggc ggttgggcga
     1441 agactgaggg agggtgtttg cggttccgtc tctatgatca ggctgctttg gcgggccgcc
     1501 agcgaaggcc ggcgttcagg atttcttgga gtaactctgt gtaatgatag cccgccttct
     1561 tagccgaatc ggcaaagtct tcgtcttccg cgatctgagg gttgggattg gcctcaagga
//
LOCUS       DATMTI010000919         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_544205, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000919 DATMTI010000000
VERSION     DATMTI010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544205"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..269
                     /locus_tag="VN039_14455"
     CDS             132..269
                     /locus_tag="VN039_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47200.1"
                     /translation="MRKIVELLDSEQRFMLLWLTDFGIAVFSSGLTVVVLKDIVSWLT
                     S"
     gene            673..1095
                     /locus_tag="VN039_14460"
     CDS             673..1095
                     /locus_tag="VN039_14460"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWV47201.1"
                     /translation="MRLIPAVLVACTLASPIAIAQTTPATTPSTAAASVAATGQWRAS
                     KLVGVDIYNEQNDKIGDVNDVIIDSSGKVDGVVVSVGGFLGMGEHDVMMKLDQIKFSN
                     KAGTTTTGSTSNSKQWYPDRGTVNATKDQLKAMPQFKY"
     gene            complement(1508..>3403)
                     /locus_tag="VN039_14465"
     CDS             complement(1508..>3403)
                     /locus_tag="VN039_14465"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV47202.1"
                     /translation="LDFVYQPIAADNGETTGIFIQGHDVTEQRVIEQAIRAESRKLDV
                     LNRTGAAVAAELDIDKIVQIVTDACTELVNAQFGAFFYNVINERGESYMLYALSGVSR
                     ANFDKFPMPRNTAVFAPTFKGTGVVRSDDILKDPRYGKNDPHYGMPKGHLPVRSYLAV
                     PVTSRSGEVIGGLFFGHGEPGQFIDEHEELLIGIAGQAATAIDNARLFQAAERELSER
                     RRAEEALQALNNTLEQRVLEEVYERSKVEEQLRQVQKMEAVGQLTGGIAHDFNNMLAV
                     VMSALNLLQRKLARGENDLDRFIDAAMDGAQRASTLTQRLLAFSRQQPLSPELLVINK
                     LVAGMSELLARSLSEQIKLETVLAAGLWQVKADPAQLENAILNLAVNARDAMPDGGKL
                     TIETMNAFVDDTYAREYAIDAGQYVLIAIADTGAGMTPDVMTKAFDPFFTTKAVGKGT
                     GLGLSQVYGFVRQSGGHVKIYSELNVGTSVKIYLPRHYDEADTTEEVKQIAAVHGGLL
                     SEVILVVEDDARVRALSIEALKELGYSVVEAAHPAQALQLLDEGRPINLLFTDVVMPD
                     MSGRELVDRARQKRPELKVLYTTGYTRNAIVHNGMLDPGTNLLSKPFSIEQLAAKVRK
                     ILDEETP"
BASE COUNT          677 a          927 c         1011 g          788 t
ORIGIN      
        1 ggcgaaggtt ggttctctca aacaaggcca tcgccgccta ccaaggattt ctcgccgaaa
       61 aaactcttac gctagtgtga ctggaactgc gcggcgtctt cccggattag agggaacagg
      121 agttatgcgc gatgcgcaag atcgttgaac ttctcgattc agagcagcgc tttatgctgc
      181 tctggttgac ggatttcgga atagcggtct tttcatccgg gctgaccgtg gtggtgttga
      241 aagacatcgt cagctggtta acgagctaaa aggggccata gagcgcgccg tgcggcagcg
      301 gtccgacaga gctggtttcg gacgcctgcc gctttcgtct gttcactggc gtcagggaag
      361 tgcctccccg ctgtgtctag ctggaaatcg aaatgccggc ggccctcacg tttatgaccc
      421 ggtcaggtta ttcatcttcg cttgcgagcg gagctgcctc acttctctat aagtccgtgg
      481 ctgacgggga cgcttgtttt tcggtctaac cgccggtcga tctttcttgc tgattgggga
      541 gctcggcggt tttcattctg cttaggccat tcacgtcctc gtttgaaaac attcggtgag
      601 ccatcgacgg gaatgtttcg gaactattgc agcgcactcc cgttgagttg catcaccgaa
      661 tgggagaaca ccatgcgatt aattccagca gttcttgttg cttgcaccct cgcatcgccg
      721 atcgcgatag ctcagacgac gccggcgacg acgccctcta cggcggccgc gtcagtcgct
      781 gccaccggtc aatggagagc gtcaaagcta gttggcgttg atatctacaa cgagcagaat
      841 gacaagatcg gcgacgtcaa cgacgtaatc atcgattcct cgggcaaagt tgacggcgtc
      901 gtcgtgagtg tcggcggttt cctcggcatg ggcgaacacg acgtcatgat gaaactcgat
      961 cagatcaagt tttcgaacaa agccggcact acgactactg gctcgacctc gaactcgaag
     1021 cagtggtatc ccgaccgcgg aacggtgaat gccaccaaag atcagttgaa ggcgatgcct
     1081 cagttcaagt actgaggtga cgatgcatta acgtgcaaca ggggtcggaa attccggccc
     1141 ctgttcgttc tttgggaacg gaatcgagtc aaacctagag cgcgtgcatc gcgagcttcg
     1201 cttcaagcgc agatgcacga tgggctggct caagcgtcag gatcggatgt cctgccgagc
     1261 tcgccctcca tgcagccata gtcttttcat cggagagcgg atcgcattcg gtagatcgca
     1321 gcgcccgcgc cccttgccgc caggcggcag ctagaaagga aaggccgccc gccgaggcgg
     1381 tctttccttt ctctatccgc tttatgcgtg ataccagcgt gcgatgcttc ggaagttgcc
     1441 cggggtattg ccctgcgtct cgatgtgagc gagcgtttcc ttggagacct cgacgaccga
     1501 tggaaaacta gggagtttcc tcatccagaa tcttccgaac cttcgcggcc aattgctcga
     1561 tgctgaacgg tttgctcagg agatttgtac cgggatcaag catgccgttg tgaacaatgg
     1621 catttcttgt atagccggtc gtataaagaa ccttcagttc gggacgtttt tgccgcgcgc
     1681 gatcgacaag ttctcgtcct gacatgtccg gcatgacgac gtcagtaaag agcagattga
     1741 tgggtcgtcc ttcgtcaagc aattgcagtg cttgtgctgg gtgggcggct tcgacgacgc
     1801 tatagcctaa ttctttcagc gcttcgatcg agagggcgcg aacgcgcgca tcatcctcaa
     1861 cgacgaggat cacctcactc agcaacccgc catgaaccgc ggcaatctgt ttgacctctt
     1921 cggttgtgtc ggcctcgtcg tagtggcggg gcaggtagat cttgaccgac gtacccacgt
     1981 tcagttctga gtatattttg acgtggccgc ctgactgacg aacgaagccg tagacttgac
     2041 tgagtccgag gccggttccc ttgccgacgg ccttggtcgt aaagaaagga tcgaaagcct
     2101 ttgtcatgac gtccggcgtc atgcccgcgc ctgtgtcggc aatggcgatc agcacgtatt
     2161 gaccagcgtc gatggcatat tcccgggcgt aagtatcgtc cacgaaggca ttcatggttt
     2221 caatcgtcaa tttgccgccg tcgggcatag cgtcacgagc attcaccgcc agattgagaa
     2281 tagcattttc gagctgagca gggtcggctt tgacttgcca cagtccggcg gccagtacag
     2341 tctcgagctt gatctgttcg ctgagagatc gtgccagcag ttcgctcatg ccggcgacga
     2401 gcttgttgat cacaagcaac tcgggtgaca gtggctgttg gcgcgagaag gccaagagcc
     2461 gttgagtaag cgtggacgcc cgttgcgccc cgtccatggc ggcatcgatg aaccggtcga
     2521 ggtcgttctc gcctcgcgcc agcttgcgct gcagcagatt tagcgcgctc atgacgacgg
     2581 cgagcatatt gttgaagtcg tgggcgatgc cgccggtgag ctgaccgacg gcttccattt
     2641 tctgcacttg ccgtaactgc tcttcgactt tcgagcgctc gtagacttcc tcgagaacgc
     2701 gttgctccag cgtgttattc aaggcttgca gggcctcttc ggcgcgacga cgctccgaga
     2761 gttcgcgttc ggcggcctga aagagccggg cattgtcgat ggcggtggcg gcctggccgg
     2821 ctatgccgat cagaagctct tcgtgttcgt caatgaattg tccgggctcg ccatgaccaa
     2881 agaaaagtcc accgatgacc tcgccggacc gggaggtgac gggaacggcg agatagctcc
     2941 gcaccggcaa atgccctttg ggcatgccat agtgcggatc attcttgccg tagcggggat
     3001 ccttgagaat gtcgtcggaa cggacgacgc cggtgccctt gaaggtcggt gcgaatacgg
     3061 ctgtgttgcg cggcatcgga aatttatcga agtttgccct cgaaacgccg gagagcgcgt
     3121 aaagcatata gctttcgccc ctctcattga tgacgttata gaagaaggct ccaaattggg
     3181 cgttgacgag ttcggtgcag gcgtcggtga cgatctgcac gatcttgtcg atatcgagtt
     3241 cggcggcaac ggcggcgccg gtgcggttca acacgtcgag tttgcgggat tcagcccgaa
     3301 tggcttgctc gatcacccgt tgttccgtga cgtcgtggcc ctggataaat attcccgtcg
     3361 tttctccatt gtcggccgca atgggctgat agacgaaatc gag
//
LOCUS       DATMTI010000920         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_205279, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000920 DATMTI010000000
VERSION     DATMTI010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205279"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..2276)
                     /locus_tag="VN039_14470"
     CDS             complement(237..2276)
                     /locus_tag="VN039_14470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWV47203.1"
                     /translation="MNEDMVESPPFSEPVITPPWERNGDVNAFLYLEWLVTNGLGGYS
                     SGTVMQIATRRYHGIFVPDLASPRGRTIIIPRLDEELDVGGTTVRLSGAEHADGRLDT
                     DIFKHLTAFRREWQTPTWKFSCHGREVHKRVIMPYGQNTVYVEYRLLAGEPVTLRLRP
                     FITFRMPDAPLSEARRGPFLLTVMRERYEVQVCEGVAPLKLGLRPHAGVFVMNSHISH
                     DVSYRIDRDRGSLHVEDLESPGYFTVQLRLDHPVAFVASMEPWENLQHNPDAIFDAER
                     ARLLKLIPQAETADPFETKLALAADQFIVFPGSRMEEHAFALASGDEARTVIAGYHWF
                     TDWGRDTMISLEGLTLCTGRHREARAILRTFARYIKDGLIPNLFPEGERTGLYHTADA
                     TLWFFHALDRYYEVTGDRDTLMVLYPALKEVMEHHVTRTKFGIGVDEQDGLLKADAPG
                     YALTWMDAKAEDWVVTPRRGKPVEIQGLWYNAVRLMGQWADELGEGSDRWGGLAKQIE
                     SSFNDRFWYASGGYLYDTVDGEAGDDPSLRPNQILTMSLRYPLLQKDRWAPVLAVVRD
                     KLLTPFGLRSLAPGHRDYKPMYFGDLRARDAAYHQGTVWAWPIGHFIDASLKAGVSRA
                     ECRRMLNAFDMHLADDGIGTISEIFDADPPYAPRGCIAQAWSVAEVLRAYQATRD"
     gene            complement(2273..>2702)
                     /locus_tag="VN039_14475"
     CDS             complement(2273..>2702)
                     /locus_tag="VN039_14475"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HWV47204.1"
                     /translation="FERSKLDRAERITHGPIYRLYRDLLRLRREDPVFSAQSRENLDG
                     AVLSSHAFALRYFEETGYDRLVVINLGADLHFIPAPEPLLAPRPDGYWAFLWSSDHPQ
                     YGGPGIVNPLTDEGWHISAESATVFRIEQDVAAVTHERTQ"
BASE COUNT          588 a          806 c          730 g          578 t
ORIGIN      
        1 ctattcaacc ttgaaaaaac atggagcaca tcgtccgcaa ggctttgctc gcgaccaaac
       61 cttgccgtcc tgctcggaca atctgtctag cgaaggacaa tccgtattgg gaatacccga
      121 cgtcgcggga tagtacagcc gtcgtcaatg tttacactgt caacccgatg tcccgattcg
      181 cagttccata cttatcggtt ccctggcagg gtaaactcca gagtttaccg tacggtttag
      241 tctctcgtgg cctggtatgc ccgcagcact tcagcgacac tccaggcctg tgcaatgcat
      301 ccgcgtggtg catagggagg atcggcatca aaaatttcgc tgatggtccc gatcccatca
      361 tccgccagat gcatatcgaa agcgttcaac atccgacgac actccgcacg actcactcct
      421 gctttcagcg aggcatcgat gaaatgaccg atcggccagg cccacacggt gccttgatgg
      481 taagcggcat cccgtgcacg gaggtcgccg aagtacatgg gcttatagtc ccggtgaccg
      541 ggtgccaggc tgcgcaggcc gaacggagtc aagagtttgt cgcgtaccac tgcgagaaca
      601 ggtgcccatc ggtccttctg caagagggga tagcgcagag acattgtgag gatttgattg
      661 gggcgtagac tcgggtcatc accggcttca ccgtccacgg tatcgtacag atacccgccg
      721 gatgcatacc agaagcgatc gttgaaagag ctctcgatct gttttgccaa tccaccccag
      781 cggtccgatc cttctcccaa ctcatcggcc cattgcccca tcaaacgaac ggcgttgtac
      841 cacagtccct ggatctccac cggcttgccc cgccgtggcg taaccaccca gtcctccgcc
      901 ttggcgtcca tccacgtcaa cgcatagccc ggggcgtcgg cctttaagag cccgtcctgc
      961 tcgtcaacgc cgattccaaa cttggttctt gtcacatgat gctccatcac ctctttcaat
     1021 gccggataga gcaccatcaa cgtatcccgg tcgcccgtca cctcgtaata ccgatcgagc
     1081 gcgtggaaga accacaaggt ggcatccgcc gtgtgataca gtcctgtgcg ttccccctcg
     1141 gggaatagat ttgggataag gccgtctttg atgtaacggg cgaatgtgcg caggatcgcg
     1201 cgagcttctc gatgccgccc agtgcacaat gtgagccctt ccaagctgat catcgtgtct
     1261 cgtccccagt ccgtgaacca gtggtatccg gcaatgaccg tccgcgcttc gtcgccggag
     1321 gccagcgcaa acgcgtgctc ctccatacga ctgccgggaa acacgatgaa ctggtccgct
     1381 gcaagggcaa gctttgtttc gaaggggtca gcagtttcgg cttgagggat cagtttcaat
     1441 aatcgagcac gctcggcgtc gaatatggca tcggggttgt gctggagatt ttcccatggc
     1501 tccatgctgg ccacgaacgc gaccggatga tctagtctca gctgcacggt gaaatagccg
     1561 ggactctcga gatcctcgac atgtagggat ccacgatccc tgtcgatccg atatgacacg
     1621 tcatggctga tatgcgagtt catcacaaac acaccggcat gcggtctcaa accgagtttc
     1681 aagggagcta ccccctcgca tacctgcact tcataccgtt ctctcatgac cgtgagaaga
     1741 aatggtccgc gacgtgcctc actgagtgga gcatcaggca tgcgaaaagt gatgaagggc
     1801 cgcagtcgca aggtgacggg ctctcccgcg agcagccggt attccacata gacggtattc
     1861 tggccgtacg gcatgatgac ccgcttgtgt acctcgcgcc catgacatga gaacttccat
     1921 gtcggagtct gccattctcg acgaaaggcc gtaagatgtt tgaagatgtc cgtgtccaat
     1981 ctgccgtctg catgctccgc tccgctcaac cgaacggtcg tcccaccgac atcgagttct
     2041 tcatcaagtc tgggaattat gatggtacgt cccctcggcg aggctaagtc ggggacgaat
     2101 attccgtgat aacggcgcgt cgcaatttgc atgaccgtgc cggatgagta ccctcctaat
     2161 ccgttcgtca ccagccactc gagatacaag aatgcattca catccccgtt tcgttcccaa
     2221 ggcggagtga tgaccggttc ggagaaaggc ggtgactcaa ccatgtcttc gttcattggg
     2281 tccgctcgtg ggttactgcc gcaacatcct gctcgatgcg aaacaccgtc gcggactcgg
     2341 ctgagatgtg ccatccctca tcagtcagtg ggttgacgat cccgggtcct ccgtattggg
     2401 gatggtcgct cgaccataga aacgcccaat atccgtcagg ccgtggagcc agcaacggct
     2461 cgggagcggg aatgaagtgg agatccgctc ccaaattgat cacaacaaga cggtcataac
     2521 ccgtctcttc gaaataccga agagcgaaag cgtgcgaact cagaacggcg ccatcaagat
     2581 tttcgcgtga ctgcgcggag aacaccggat cttcgcgacg cagccgtaac aagtctcggt
     2641 agagtcggta gatcggccca tgggtaatcc gttcggcgcg gtcgagcttg gaccgttcga
     2701 ac
//
LOCUS       DATMTI010000921         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_288543, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000921 DATMTI010000000
VERSION     DATMTI010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288543"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VN039_14480"
     CDS             <1..249
                     /locus_tag="VN039_14480"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HWV47205.1"
                     /translation="RFSRHVLEEMGKRKISQQLVEQILHAPEQKVPEVDDITCYQSKV
                     EIGGKTYLLRIMVNEVITPAVVVTMYRTSKISKYWRQP"
     gene            246..440
                     /locus_tag="VN039_14485"
     CDS             246..440
                     /locus_tag="VN039_14485"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HWV47206.1"
                     /translation="MKVKYDEEVDVLTIEFSDAQVEESDQDKPGIILDYDKDGNIVGM
                     EILNASKRVQNPKSVEYAVA"
     gene            complement(601..1287)
                     /locus_tag="VN039_14490"
     CDS             complement(601..1287)
                     /locus_tag="VN039_14490"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HWV47207.1"
                     /translation="MVRLVLLLIVVALVLVGPQLWTRRVFAKHSAPRADYPGTGGELA
                     RHLLNRFDLQHIKVEPTEKGDHYDPVTKAVRLTPAIFDGKSLTAITIAAHEVGHAIQD
                     HLGYQPLAERTKLIRVAQGAEKVGAVLMMGIPIAAAVARTPVASVVVMMAGMATMGIS
                     TLVHLVTLPVEWDASFRRALPVLKQGQYLSPEDEQGARSILTAAALTYVAASLASLLN
                     LWRWIGFLRR"
BASE COUNT          372 a          413 c          428 g          307 t
ORIGIN      
        1 cgtttctctc ggcacgtgct cgaagaaatg gggaaacgta aaatatcaca gcagcttgtg
       61 gaacaaattc tccatgcgcc ggaacaaaag gtgccggaag tagacgacat tacctgctat
      121 cagtcaaaag tagagatcgg cggaaagacc tacttgttac gcatcatggt caatgaagtg
      181 ataacgcctg ccgtcgtagt tacaatgtac cgaaccagca agataagcaa atattggagg
      241 cagccatgaa agtaaaatac gatgaggaag tggacgtact cacgatcgaa ttcagtgatg
      301 cccaagtcga ggagagcgat caagacaagc ctggtatcat tctggattac gacaaggatg
      361 gaaacatcgt cggcatggaa atcctcaatg cctcgaaacg ggtgcagaat ccgaaatccg
      421 tcgaatacgc ggttgcttag ttgtctttcc atgtgggcga gatctgcatc cacatcctca
      481 atgacgtctt gctgaagcaa atgggccggg aatctttgct cgccgcacgc catcaccgac
      541 gacctcgaag ctgtttggga tcagttctat ggagtaggag aatgcgcgaa ggggccgggc
      601 ctatcgtcga aggaacccaa tccaacgcca caggttcagc aggctggcga gggaggccgc
      661 cacgtaggtc agcgcggctg cggtgagaat gctgcgagcc ccttgctcat cttcaggcga
      721 caggtactgg ccctgcttga gaacggggag ggcacgccgg aagctggcgt cccactcgac
      781 gggtagtgtg acgaggtgga ccagcgtaga gattcccatc gtcgccatgc cggccatcat
      841 gacgacgaca ctcgcgaccg gcgtgcgagc caccgccgcc gcgatcggaa ttcccatcat
      901 caggaccgct ccgaccttct ctgcgccctg cgcgacccgt atgagcttgg tgcgttccgc
      961 aagcggctgg taacccaggt gatcttgaat ggcgtgcccg acctcgtggg cggcgatcgt
     1021 aatcgccgtg agggatttcc catcgaagat agcgggtgtc aggcgaaccg ctttggtgac
     1081 cggatcgtag tggtcgccct tctcggtcgg ttcgactttg atgtgctgca ggtcaaaccg
     1141 attgagcagg tgcctggcca attctcctcc ggttcccgga taatcggcac ggggcgcact
     1201 atgcttggca aacacccgcc tggtccaaag ctgcggcccg acaaggacca gagcaaccac
     1261 gatcaagagt aggacgagac gcaccataaa tatcccgatc tccccgccac gctctacaca
     1321 ggctaacaca attgaagaca ggaactgaaa atgcgactag atttcccttg ctccatggca
     1381 ctattgttca ccacgatccc ggtttctcca taaccttcgg tgggtttctc caaaccggtc
     1441 tgctatactc ggtgcaagga gatactgtat atgaaggaac aattgaccgc cgtgtttcag
     1501 caagtgacgg aaggctatat
//
LOCUS       DATMTI010000922         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_841960, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000922 DATMTI010000000
VERSION     DATMTI010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841960"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..472
                     /locus_tag="VN039_14495"
     CDS             65..472
                     /locus_tag="VN039_14495"
                     /inference="COORDINATES: protein motif:HMM:NF025444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HWV47208.1"
                     /translation="MGAWGNGSFDNEDAADFLADVTEASDLTAVREIFAAVLSADEYL
                     EAPDASQAIAGAEIVAAALGRPTPAAQQEEELAEWIARAKPVADPELAKQAIQVLDRI
                     LAPGSELHELWEESGELAEWQAVVEDLRSHLQA"
     gene            615..1166
                     /locus_tag="VN039_14500"
     CDS             615..1166
                     /locus_tag="VN039_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47209.1"
                     /translation="MTMQARHYMPAILAFSSFVFGAALLAYEHFNGGVQSNYLLDRPD
                     LPLISNWYGLIVLPLLGWLLGVRLRNHPTLSTRLWLPKDIWAGLVCSLLYGAALATSC
                     ELAPFTITSALLLGMLLLAVALPIYRVECIFGFVVGMTFTFGAVLPALVAVVFAAVSV
                     VARFAFRAVMSAIRRPNQPPGVA"
     gene            1313..>2028
                     /locus_tag="VN039_14505"
     CDS             1313..>2028
                     /locus_tag="VN039_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47210.1"
                     /translation="MIRIDDHRIRAYVRPPEECGEKGLVHHAHHVIKALVLSLNIASL
                     GHFYWESCSSANPILSLTESLEPSGAKELMLVTRPPVPDFGQLRSFSPNDEYRSVMLF
                     GVFARGSAHVLEDEYCRGLLLFHMQFCDIDFRRDAFMCFYRVLEHFITRRVLGKPRLQ
                     NELKDIQLCISRLGFDGDLADEMKELYLLRSGQTAHAQNTPRPITGDEVMKMKIFVDA
                     LLVKILLAEADGIMEKTHGSR"
BASE COUNT          423 a          557 c          550 g          498 t
ORIGIN      
        1 gtcattcaag gcggacggct acgccgccgc ttaattccag cgttaggcct acagggagag
       61 agaaatgggg gcttggggaa acgggtcgtt tgacaacgaa gatgcagcag acttccttgc
      121 ggacgttacg gaggcttctg atctcacggc tgtccgcgag atcttcgccg cagttcttag
      181 cgccgacgag taccttgagg caccggatgc aagccaagcc attgcaggtg cggagattgt
      241 ggcagctgcc ctgggccgac cgactcctgc cgcgcaacag gaggaggagc ttgctgagtg
      301 gattgcgcga gccaagcccg ttgctgatcc tgagctagct aaacaggcga tacaggttct
      361 tgatcgcatc ctcgcacccg gctcagaact gcatgagctt tgggaggagt ctggcgagct
      421 tgcggagtgg caagcagtag tcgaggacct ccggtcgcat ctgcaggcct aacaatgcgt
      481 tcaagccgaa attgcatcgc tacgccgtcc acatggcaga aagagcttgc catgtggcca
      541 gctacgcgct gcaattcggc ttaacttagg cgttaggccc cacaaccacc atctcagcaa
      601 ggatgtgaca caagatgacc atgcaagcac gccactacat gcccgcaatt cttgcgtttt
      661 cctcgtttgt gtttggagct gcgctacttg cctacgagca cttcaacggc ggcgtgcaga
      721 gcaactactt gctggatcgc cctgatttac cactcatctc caactggtat gggctgatcg
      781 tcctgcctct tctcggttgg ctgctaggcg ttcgacttcg taatcaccca accttatcta
      841 ccagactttg gttgcctaaa gacatttggg ccggtcttgt ctgctcttta ttgtatggcg
      901 cggcattggc gacatcctgc gagcttgccc cattcacaat cacctctgcc ctgctccttg
      961 gcatgctttt actcgcagtc gcgcttccta tttaccgcgt cgagtgtatc ttcggattcg
     1021 tcgtcggcat gactttcacg ttcggcgctg tgctccccgc tctagtggca gttgtatttg
     1081 cagcggtctc agtcgtggcg cgtttcgcct tcagggcggt gatgtcggca atacgacgtc
     1141 ccaaccaacc gccgggtgtg gcctaacatc tcattcgagg cggacggcta cgccgccgct
     1201 taactccagc gttagccatc atatgtgggc ttcttgcatt ctcagatccg acgcacacgt
     1261 catcgaagcg ggttcgcttc ggctattgca gtccgaagag ctgaacatcc gggtgatacg
     1321 aatagacgat cacaggattc gcgcttatgt tcgcccgcct gaagagtgcg gggaaaaggg
     1381 tctcgtacat cacgcgcatc atgtaatcaa agcattagtt ttatctttga acatcgcctc
     1441 ccttggccac ttctactggg agagctgctc gtcggcaaat ccgatcctaa gccttacgga
     1501 aagtttggaa ccctccgggg ccaaggaact tatgctcgtc acgcggccac ccgtacccga
     1561 ttttggccag cttaggtcgt tctccccgaa cgacgagtac cgctcggtca tgctgtttgg
     1621 tgtgtttgcg cgaggatcag cacatgtgct cgaagatgag tattgtagag gcttgctctt
     1681 gttccacatg caattttgtg acatagattt tcgccgcgat gcgttcatgt gtttctaccg
     1741 cgtacttgag catttcatta cgagacgagt cttgggaaag cctaggttac agaatgagct
     1801 caaagatatt cagttgtgca tctcacgact tggcttcgac ggagacttgg cagatgaaat
     1861 gaaagagctc tacttacttc gcagtggcca gacggctcat gcccagaaca caccacgtcc
     1921 aatcacaggc gatgaggtca tgaagatgaa gatttttgtt gacgctttgc ttgtcaaaat
     1981 tctgctcgca gaggcagatg gcatcatgga gaaaacgcat gggtcacg
//
LOCUS       DATMTI010000923         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_841968, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000923 DATMTI010000000
VERSION     DATMTI010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841968"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VN039_14510"
     CDS             complement(<1..737)
                     /locus_tag="VN039_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47211.1"
                     /translation="MSGAIRSGLSHLLIAAVIIESLAGCLPSLQASQRAQADALLSRC
                     RPPRDVPQLRGSLSVPRWTGAGIEAVIPVSLLSIEAQRTAAQIGFLDLPERSSLKQDD
                     RKERHALAMISLRQALSNRIALVVSDVMVTIAALDCEVARSDHLANAITEAHQDVSDR
                     ALFAVLASDIFIGVIPGALMLSGLGIAAEANEVFGGVAATSFGSVDRVLHIDEDFRHP
                     HNFLQELWDGPSESQLFPPVVWRFLNDT"
     gene            complement(734..1897)
                     /locus_tag="VN039_14515"
     CDS             complement(734..1897)
                     /locus_tag="VN039_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47212.1"
                     /translation="MAALLRGGLAPHDFVVLVQVRLGLSGLHALVSLWGLTVSSRIAM
                     NKPVRSFQRTQIFTTPIACLLCVLTLEGCVTGASGMARFRFEAMESRCTGPTIEQLTA
                     LPALPVLPGSEDNPDGAIPLSPASSRLATLLGLDRALRDLSTLQRDHAANAGPSSEFL
                     WRNEQIMNRISLASFDVVSTVTELDCEEARSDHVADGLTELRQDKQERGLFLALVGDA
                     FIGVVAGSLSLAGKATAAAANAILGGVLATGIGGAATIFLSVDHEFLHPRNHLAELSE
                     AKEFSPLFPDSVWRYLTTSPDPSEGTIRDELLRRWERDGRLGVPGSEDARRRETLLLG
                     AGGTYDIRDLRVRSEMLNHLKSAVLQMGQDLNALMYELMAVQYPLPAMSEASQ"
     gene            complement(2136..>2345)
                     /locus_tag="VN039_14520"
     CDS             complement(2136..>2345)
                     /locus_tag="VN039_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47213.1"
                     /translation="IGGAPRVLPHLTAQCLHIWNTRELEMADFSYYGGAMGHHPPEEE
                     TFCLGDDNEGSFPVALAQVVRMFAL"
BASE COUNT          569 a          671 c          661 g          444 t
ORIGIN      
        1 gtgtcgttta aaaatcgcca gaccaccgga gggaacagct gtgactctga gggcccgtcc
       61 caaagttctt ggagaaaatt atgcggatgc cgaaagtcct catcgatgtg cagcactcta
      121 tccacactgc cgaagctcgt agccgccacg cctccaaaca cttcattggc ttctgcggca
      181 atccccagac ctgacaacat aagggcgccc gggataacgc cgataaagat atcggatgcg
      241 agtacagcaa acagggctcg atccgaaacg tcctgatgtg cctccgtgat ggcattggcc
      301 aggtgatcac tccgtgccac ttcacaatcc aaggccgcga tggtgaccat tacatctgag
      361 accacgagtg caatgcgatt agataatgcc tggcgaagcg aaatcatggc cagggcgtga
      421 cgctctttcc tatcgtcctg tttcagagag gatcgctcag gcaaatcaag aaatccgatc
      481 tgagccgcag tccgctgagc ctctatagat aagagagaga cagggatgac tgcttcgatc
      541 ccagcccctg tccaccgagg aacggagagg ctgccgcgaa gctgggggac atctctggga
      601 ggacggcaac gggagagcag cgcatctgcc tgcgcacgtt gcgaagcttg caaactagga
      661 aggcacccag cgaggctttc aatgatgacc gcagcgatga gcagatgact gagaccggag
      721 cgaatggcgc cgctcattga gacgcctcac tcatcgcagg gagagggtac tgaacagcca
      781 tgagttcgta catcagggca ttgagatctt ggcccatttg gagaactgca gacttcaggt
      841 ggttcaacat ttccgagcga acccgcaaat ctcgaatgtc atacgtgcct ccagcaccga
      901 gtaggagtgt ctcgcggcga cgggcatcct cggatcccgg aacacccaag cggccatcac
      961 gctcccaccg ccttagtaac tcgtcccgaa tcgttccctc tgaaggatcg ggcgaggtgg
     1021 tgagatatcg ccacaccgag tcaggaaaga ggggcgaaaa ctccttcgct tccgacagtt
     1081 cggcgagatg attgcggggg tgcaaaaatt catgatctac agagagaaaa atcgtcgcag
     1141 ccccgccgat cccggtcgcc aagacaccgc caagaatcgc attcgctgcg gcggctgtcg
     1201 ccttgccggc caacgacagc gagccggcga ccaccccgat gaaggcgtct cccacgagtg
     1261 ccaggaacaa accccgctct tgcttgtcct gtctgagctc ggtcaggcca tccgcgacat
     1321 ggtcagagcg ggcttcttca caatcgagtt ccgtcacagt gctgacgaca tcgaatgacg
     1381 cgagggaaat acggttcatg atctgttcgt ttcgccataa gaattccgag cttggcccgg
     1441 cattcgccgc gtgatctcgt tgaagggtcg aaaggtcgcg taaggcacgg tccaaaccga
     1501 gaagtgtcgc caatctcgac gaagccgggg acaaggggat agcaccgtcc gggttgtctt
     1561 cgcttcccgg aagcacgggc agcgccggaa gggcggtgag ttgttcaatg gttggtcccg
     1621 tgcagcgact ctccatcgcc tcgaagcgaa atcgagccat gccggacgca ccggtgacgc
     1681 aaccttccag agtgagcaca cagagcagac aggcaattgg tgttgtaaaa atctgagttc
     1741 gctgaaaact cctcaccggc ttattcatgg cgattcgtga cgaaacagtc aagccccaaa
     1801 gtgagacgag tgcgtggagc cccgagaggc caaggcgtac ttgaaccaat acaacgaagt
     1861 catgaggggc gagcccgccc cgcagcaacg ctgccattaa tcgcgctcca tcttcgatga
     1921 gtcaagcaac atgccgctca atcacgtgga gggaggcacc gtctggtctt gcggtgagaa
     1981 ccgacaaaat ggcgtctcga acctcccagg acgccgcttt ttgttggagg gctggtttgt
     2041 acacggagcg gaggagaaga gacgtctaga cgtctttgtt aatgaccatc attcaactcc
     2101 ggccaagcac ggacgtatca gcctgagtta caacactaca gagcgaacat gcgtaccacc
     2161 tgagccaggg ctactggaaa agatccctcg ttgtcatctc ccaggcagaa tgtttcttct
     2221 tccggaggat gatgtcccat ggcgcctcca taataagaaa agtccgccat ttccaattcc
     2281 cgggtattcc agatgtgaag acactgcgcc gtcaggtgcg gcagcactct cggagctcca
     2341 ccgat
//
LOCUS       DATMTI010000924         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_34181, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000924 DATMTI010000000
VERSION     DATMTI010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34181"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..368)
                     /locus_tag="VN039_14525"
     CDS             complement(42..368)
                     /locus_tag="VN039_14525"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HWV47214.1"
                     /translation="MARNSHEQPYRARALKILPWICAHCGREFDGKKLSQLTVHHKDH
                     NHDNNPPDGSNWELLCLYCHDNEHQRYLVAETPGETPSNREQERPSTFTPFAHLEDLL
                     KRKHIA"
     gene            complement(501..1181)
                     /locus_tag="VN039_14530"
     CDS             complement(501..1181)
                     /locus_tag="VN039_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HWV47215.1"
                     /translation="MRHSEPHRIHRTGWLRAAVLGANDGIVSTSSLVLGVSAAGASSQ
                     NIVIAGVAGLVAGAMSMAAGEYISVSSQVDTERADLDRERKELAADPEHEHAEMTAMY
                     VARGLDHNLASAVTTQLMAHDALGAHSREELGLTDTMASRPVQAALASAGTFSVGALL
                     PLLIVVAVPRATIIWAVSVSSLIFLALLGSLAAHTGGASVMTGAARVTFWGALAMAVT
                     AGVGGLLG"
     gene            complement(1541..1909)
                     /locus_tag="VN039_14535"
     CDS             complement(1541..1909)
                     /locus_tag="VN039_14535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV47216.1"
                     /translation="MDVRVRSLEEVRKFYAILLPAPGFTRQRQVDDWLSFEAADSGKA
                     TEFFGITESAHHVPNECRIAFWAESTRDVDRLADIAVKAGARNVEGPGYDEGAGYYAV
                     FFEDPSGNRLEICHREDEAA"
     gene            complement(2006..3031)
                     /locus_tag="VN039_14540"
     CDS             complement(2006..3031)
                     /locus_tag="VN039_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWV47217.1"
                     /translation="MPEPDITSLPPHAQLVQMGTAHWVSHILYVAAKLSLADHLAKGP
                     ARTDDLAAMTNTHSPSLGRLLRTLGHLGLVTEDGAGRFTLTLLGAALKNGAPGSARAA
                     ILTLASPIMTQGWGHLLESVQTGKPGLEQAMGMPIFDWLARHPEEASLFSETMVSFHG
                     AESSAVAAAYDFSDMNTIVDVGGATGNLLAAILSQYPKPRGILYDLPHVVRDAPALLQ
                     SRGVADRVTIESGSFFDCVPTSCDAYLLSHIIHDWTEARCLTILGHCRRALTPAGRVL
                     IIEMVLPEDNTPHPGKMLDMMMLVGPGGQERTIPEYRQLLDKAGLRLTKVVPTDSAVS
                     IVEAMSV"
     gene            3264..3902
                     /gene="tmpT"
                     /locus_tag="VN039_14545"
     CDS             3264..3902
                     /gene="tmpT"
                     /locus_tag="VN039_14545"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HWV47218.1"
                     /translation="MDSSFWHQRWEKNEIGFHEGKPNQLLVKHFHELSVATGRRIFVP
                     LCGKTQDISWLLSRGYHVAGAELSQLAIEQLFMELGMQPEIATVDSVEQWSANNLDIF
                     VGDIFTVSGKMLGPVDAVYDRAALVAFPEGLRKRYTVHLTEVTKKSPQLLICYEYDQS
                     LMDGPPFSVSNQEVHRHYATTYDLTLLSSTEVAGGLKGKGPAKEDVWLLKRK"
BASE COUNT          892 a         1041 c         1143 g          845 t
ORIGIN      
        1 aaagaatgcg cagggacagg ggccccaatt tttatggttt attaagctat gtgtttgcgt
       61 ttgagcaaat cctcgaggtg ggcgaagggc gtaaaggtcg aaggtcgttc ttgttcccgg
      121 tttgatggtg tctcgccagg cgtttcagct accagatatc gctggtgttc attgtcatga
      181 caatagagac acagcaactc ccagttgctg ccgtcagggg gattattatc atggttgtgg
      241 tccttgtgat ggaccgtgag ttggctgagc tttttaccgt cgaactctcg tccacaatgt
      301 gcgcagatcc aggggagtat ttttaacgcg cgtgctcgat aaggctgctc gtgagagttc
      361 cgtgccatgc gagcctccag gaaaagggcg actcggcaca caataattat acacccatct
      421 tggactaaac atgccatgga tcttccgcgc cgttggttca acacatcgaa gcgggttgtg
      481 agcgctttca ccttaggacc ttatcccaac aagcccccaa ctccggccgt gacagccatg
      541 gccaaggcgc cccaaaaggt gacccgtgca gcacccgtca ttaccgacgc gccgccggta
      601 tgtgcagcca gcgagccgag cagtgcgaga aaaataagtg agctgactga cacagcccat
      661 atgatcgttg ctctcggcac tgccacaacg atcaaaagag ggaggagtgc accgactgaa
      721 aacgtgccgg ccgaggcgag cgcggcttga accggtcgag aagccatggt gtcagtgaga
      781 cccagttctt cacggctgtg ggcgcccagt gcgtcatgtg ccatcaattg ggtcgtgaca
      841 gcagaggcga ggttgtgatc gagtccacgc gctacgtaca tggcggtcat ttctgcatgt
      901 tcgtgctcgg gatcagcggc caattccttg cgttcccgat ccagatcggc gcgttccgtg
      961 tcgacttgcg aactgactga gatatattcc ccggccgcca tggacatggc gccggccaca
     1021 agtccggcca cgccggcaat cacaatgttc tgtgaactcg ctcctgccgc cgacacgccc
     1081 aagacgaggc ttgacgtcga gacaatcccg tcattcgcgc caagcacagc tgcgcggagc
     1141 cagccggtgc gatggatcct gtgcggttca ctgtgtctca cgatgttcgc ttctttcagc
     1201 gatcagggat tgtgcaacca cttatggtgg gcaattgcct caattgccag gaatcagtct
     1261 ggtgcagccc tgaatcccct ggaaactcta taacacaaaa cgaatctatg aatgtgtttg
     1321 ccatgcaacg ctggtttgca aatgacgaac catcttgcgt gactgtccct tgatatgggg
     1381 caatccgcac agccggaatt gagggaggag gaaaaagata tggcttccgg ctggaacgcg
     1441 agaagaatgg ttaacggatg agaatgggat cagggtgtcg gtgaatgaag ctcaacgtca
     1501 ttctggatga gcgaagacga tctgtcgttt acaaatcatc ctatgcggct tcgtcttctc
     1561 ggtggcagat ctcgagacga ttgccgctcg gatcttcgaa gaaaaccgca taatatcctg
     1621 caccttcatc ataaccgggc ccttcgacat ttcgagcgcc tgccttgacg gcgatgtcgg
     1681 caagtcggtc cacgtcacga gtactctccg cccagaacgc aatccggcac tcattgggaa
     1741 catggtgagc ggattctgtg atgccaaaga attccgtcgc cttcccggaa tcggccgctt
     1801 caaacgatag ccaatcatcc acctgtctct ggcgtgtgaa tcccggtgcc ggcaggagga
     1861 tcgcataaaa tttgcgcact tcctccaggc tgcgaaccct cacatccata tgatcgtatc
     1921 gtcgccccat agcgagatta taatagaaga gagagatgga gaggtgagcg aggcaacaaa
     1981 ggagaggaac ctgtgagcgt atcgatcaga cgctcatcgc ctccacgatg ctcaccgccg
     2041 aatccgtcgg caccaccttt gtcaatcgta gtccggcctt gtcgaggagt tggcgatatt
     2101 ccggtattgt tcgttcttgt ccgccggggc cgacgagcat catcatgtcg agcatctttc
     2161 ccgggtgtgg ggtgttgtct tccggcagga ccatctcgat gatcagtaca cgaccggccg
     2221 gtgtgagagc gcgccggcaa tggcccagaa tcgtcaggca ccgggcttca gtccaatcgt
     2281 gaatgatgtg cgaaaggaga tacgcatcgc agctggtggg tacgcaatcg aagaaactgc
     2341 cggactcaat cgtcacccga tcggctacgc cgcgggactg gagaagtgcc ggggcgtcac
     2401 ggacgacgtg cggaagatcg tagagaatac cgcgcggttt ggggtattgg ctcagaatgg
     2461 cggccaacag attgcctgtc gcaccaccga cgtctacgat cgtgttcatg tcggagaagt
     2521 cgtatgcggc tgccacggcg gacgattccg ctccatggaa actcaccatt gtctcgctga
     2581 agagcgatgc ctcttcagga tgccgggcca gccagtcgaa aatgggcatg cccatggcct
     2641 gttccagccc gggttttccg gtctggaccg actcaagcag atggccccat ccttgagtca
     2701 tgatcgggct cgccaatgtc aggatcgcgg cgcgcgccga acctggggcg ccgttcttca
     2761 gcgccgcacc gagcagggtc aacgtgaagc ggcctgcgcc gtcttcggtc acgagtccca
     2821 ggtgtccgag tgtgcgcagc aagcggccaa gggaagggga gtgggtgttt gtcatggcgg
     2881 ccaagtcatc cgtacgggcc ggtcccttgg ccagatgatc cgccaaactc aactttgcgg
     2941 cgacgtagag gatgtgcgat acccagtgcg cggtgcccat ttgaacgagt tgtgcatgag
     3001 gaggaagaga cgtgatatcg ggttcgggca tcgtgtggcc tccgggatca aggtgcgtcg
     3061 tgttacgcgg aggagtgtac tgcaatgaag gagggatatt cactattaaa gcgagaaaaa
     3121 cggaaaggtg atgagggata acgcaccaat gtagagctgg atgacgcttg acaattgatg
     3181 aaccaacatc aagtatctca cgccagtcgc cggtgcatag gatgcgatca gttgctcaat
     3241 tgtcctgtcc aaaggagatt tacatggact caagtttttg gcaccaacgg tgggagaaaa
     3301 atgaaattgg cttccacgaa ggcaaaccca accaactcct agtcaagcat ttccatgagc
     3361 tgtcggtagc cacaggccgt cggatctttg tccccttgtg cgggaaaaca caggatatct
     3421 cgtggctgct gtcccgtggt tatcacgtgg ctggagccga attaagtcaa ctcgccatcg
     3481 agcaattatt catggaactt ggcatgcaac cggaaatcgc gaccgtcgac agcgtcgagc
     3541 agtggagcgc gaacaatctc gacatatttg tcggggatat ctttacggtg tccggaaaga
     3601 tgctcggccc ggtcgatgcc gtctatgatc gggctgcgtt ggtggcgttt cccgaaggcc
     3661 tgcgaaagcg ctataccgta cacctcacgg aagttactaa gaagtcgccc caattactta
     3721 tttgttatga gtacgaccaa agcctgatgg acggcccgcc gttttcagtc agcaaccagg
     3781 aagtccaccg acactacgct acgacgtatg atctcactct tctgtctagc actgaggtcg
     3841 caggtggatt gaagggaaaa ggaccagcca aagaggatgt ctggctgctg aaaagaaagt
     3901 gatggttcgc agggcggctg a
//
LOCUS       DATMTI010000925         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_841984, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000925 DATMTI010000000
VERSION     DATMTI010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841984"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1964)
                     /locus_tag="VN039_14550"
     CDS             complement(75..1964)
                     /locus_tag="VN039_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWV47219.1"
                     /translation="MAKVRKTWKWSPRAWFKNLKALHKILIGFSVIMIGMMAIGLIGL
                     MGLSQLKGQLSSIYDESTVGVSHIAVSSTNLSLYHNALLSVAEQRQKSDFDEAIIPLA
                     ELKRRTLTPIETYRPSATHETMDGRRDGQELDVLLFTLREYFASAESAVGALNDSFDQ
                     SLTDEQRQSMRELATMVLSTEVALWHGRSIWRFQWVAQLIRDLASELNDNGQRMAIYL
                     TKVMLGGAVGALLLAITIGYFLARSIVRNIIYVANVATQAAAGNLQARARLDSDDEVG
                     HMAKAFNIMLDRITALVSTEEERDRMQKQLVQFLVLVSDVGKGDLTKRGEVTADMFGN
                     LADGFNLMIHRFSQLMKQVRQAAERVNASASKLKDSAGQMAGTARHQADESIKALGAV
                     EQLASSMRQVTDTAGASSEAARQVLNATEAGRLAVQETVHDMQRIQLAVQRMSKQIKA
                     LGDRSLEISQIVSTIRDIANQTNLLALNAAIEAAGAGEAGARFAVVADQVRKLAESST
                     QATREIADLVKVIQSETQHAVVAMEHETQAVEAGSASALRTGDVFKEISTIAQRSAEL
                     AQSIAAAAADQTVSTDQVGRSIKDFTGGAVATQKATDSARATVEDMVTLAEGLTTSVS
                     QFKLA"
     gene            complement(2034..>2417)
                     /locus_tag="VN039_14555"
     CDS             complement(2034..>2417)
                     /locus_tag="VN039_14555"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWV47220.1"
                     /translation="VREVFEPESITPVPGMPAALVGVTNLRGTIIPLADVRAALGVVS
                     VSVLPKYAVVVRHGTQQVGILVEDVPEIRTIQSDDLVACPVRTADERLPLFSRAFTTE
                     KKLSSIVEVSRLLASLDGTVDGQSP"
BASE COUNT          503 a          729 c          618 g          567 t
ORIGIN      
        1 ctgtcgaact ccgagctcat cgatccctct ttacaccgga aggttcctcc tcactcaggg
       61 gatgagggag attctcaggc cagcttaaat tgtgacaccg aagttgtcag accttcggcc
      121 aacgtcacca tgtcctctac cgtcgcacgc gcagaatcag tggccttttg tgtcgccacg
      181 gcacctccgg taaaatcctt gattgaacgt ccgacctgat cggttgaaac cgtttggtca
      241 gccgccgccg ccgctatgct ttgagcaagc tcagcggacc gctgagcaat cgtcgaaatt
      301 tccttgaaca catcgcccgt acgcaaggcc gaagccgacc cggcttcgac cgcctgagtt
      361 tcatgctcca tggcaacgac cgcatgttgc gtttcgctct ggatgacttt caccaggtcc
      421 gcgatctcgc gtgtcgcctg cgtcgagctc tccgcgagct tccgcacttg atcggcgacc
      481 acggcgaagc gtgctccggc ctcaccggca ccggcggcct caatcgcggc gttgagagcc
      541 aaaaggtttg tttgattggc gatgtctcga atggtcgata cgatctgtga aatttccaat
      601 gaacggtcgc cgagcgcctt gatctgtttc gacatacgct gaaccgccaa ttgaatgcgc
      661 tgcatatcgt gaacggtttc ctgcaccgcg agccggccgg cttcagtggc gtttaagact
      721 tggcgggctg cttcggacga ggctcccgct gtgtccgtca cttggcgcat cgatgacgcc
      781 agctgttcga cggcgccgag tgccttgatc gattcgtcgg cttggtgtct ggcggttccg
      841 gccatctgtc cggcactgtc cttcagcttg ctcgccgagg cattgacgcg ttcggctgcc
      901 tgacgcactt gtttcatcaa ctgtgaaaag cggtgaatca tgagattgaa tccgtccgcc
      961 agatttccaa acatatcggc cgtcacttca ccgcgcttgg tcaaatctcc tttcccgacg
     1021 tcggagacca acacaaggaa ctgcacgagt tgtttttgca ttctgtcacg ttcctcttcc
     1081 gtcgaaacca aggccgtgat ccgatccaac atgatgttga acgccttggc catatggccg
     1141 acctcgtcgt cgctgtccag ccgagcgcgg gcctgcagat ttcccgcggc agcctgtgtc
     1201 gcgacattgg cgacgtagat gatgtttctc acaatgctgc gcgcgaggaa atatccaatc
     1261 gtgatcgcca agaggagtgc cccaaccgct ccaccgagca tgaccttggt aaggtatatg
     1321 gccatgcgtt gcccgttatc attcaattca ctcgccaagt ctcgaatgag ttgagccacc
     1381 cattggaagc gccatatcga ccggccgtgc cacaaagcca cctcggttga cagcaccatg
     1441 gtagccagtt ccctcatcga ctgcctttgc tcatccgtga gcgattggtc aaaactatcg
     1501 ttcaacgccc ccacggcact ttcagccgat gcgaagtatt ctctcaacgt aaaaagcagg
     1561 acgtccaact cttgtccatc gcgacgacca tccattgtct cgtgcgtggc ggacggtcga
     1621 tatgtctcaa tcggcgtcag ggtgcgcctc ttcaattcag cgaggggaat gatggcctca
     1681 tcgaaatcac tcttttgtct ctgttccgcc acgctcaaca acgcattgtg atagaggctg
     1741 agattggtgc tgctcaccgc gatgtgcgaa actcccactg ttgattcgtc ataaatggaa
     1801 ctcagctggc ccttcagctg actgaggccc atcaaaccga tcaggccaat tgccatcatg
     1861 ccgatcatga tgaccgaaaa ccctataagg atcttgtgca aggctttcag gtttttaaac
     1921 catgcacgag gcgaccattt ccatgtcttc ctaaccttcg ccatgatttt cctcctgctc
     1981 gctgctcgcg atagggcgta catttgccga cacaatcgtt cctcaatatg atgttatgga
     2041 ctctggccat cgaccgttcc atctagggac gccaagagcc gcgacacttc tactatggag
     2101 ctcagttttt tctctgttgt gaaagcccta gaaaacaacg ggagtctttc gtccgcggta
     2161 cgaaccgggc aggcgactag atcatccgac tggatcgtac gaatttccgg tacatcttcg
     2221 accaggatac cgacttgctg agtcccatga cgaacaacca ccgcatattt cggcaggacg
     2281 gaaacggata cgacgcccaa agccgccctc acatccgcga gaggaatgat ggttccgcgt
     2341 agattggtga cgccgaccag cgcagcaggc attcctggta caggtgtgat cgactcaggc
     2401 tcgaagacct cgcggac
//
LOCUS       DATMTI010000926         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_248031, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000926 DATMTI010000000
VERSION     DATMTI010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248031"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VN039_14560"
     CDS             <1..760
                     /locus_tag="VN039_14560"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWV47221.1"
                     /translation="RAGYAEADPTFDVAGIDSAHKLAILVNLAYGTPVNFKDIYTEGI
                     TSITPTDIAYAKQFGYTIKSLGIAKLVDGEIEARVHPTMLPAASPIAQVEDVYNAIQL
                     VGDAVGDVVLYGRGAGSMPTGSAVVSDVIAIGRNLLKGAVGRVPVASFQQDQRKPLRL
                     RSMEEISSLYYLRFTVVDRPGVLAQIAGELGRCGISISSMVQQGRREGQTVPVVIKTH
                     TAKERDVQTALREINRRAFVSDPTMLIRVEGKDE"
     gene            764..1822
                     /gene="thrC"
                     /locus_tag="VN039_14565"
     CDS             764..1822
                     /gene="thrC"
                     /locus_tag="VN039_14565"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWV47222.1"
                     /translation="MTRWRGVIEEYQKFLPVTEKTPVISLGEGNTPLIRAARLAKAIA
                     PGVELYLKFEGVNPTGSFKDRGMTLAISKAVESGARAVMCASTGNTSASAAAYGARAG
                     LAVYVLIPAGKIAMGKLSQAMMHQATVIQIEGNFDQALTLVKDFSTAMHIELVNSVNP
                     FRIEGQKTAAFEVCDQLGDAPALHVLPVGNAGNITAYWKGYKEYRSANQISRVPRMMG
                     FQAAGAAPIVLGRVVDQPQTIATAIRIGNPASWTSALNAIEESAGAIDMVTDEEILRA
                     YTAVASTEGVFCEPASAASVAGVAKLHRTKILREGETVVCTLTGHGLKDADTAISVSK
                     QPVTVKATREDVARLLNV"
     gene            1839..>2276
                     /locus_tag="VN039_14570"
     CDS             1839..>2276
                     /locus_tag="VN039_14570"
                     /inference="COORDINATES: protein motif:HMM:NF021646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47223.1"
                     /translation="MKYVMVHAGGMADRPRPELGGRTPLQASSTPNLDRLAQIGELGQ
                     LLTPEDGVRHGSGLIGAAILGYDPRKFYQGPGPLEAASLGVSVTEHDVVYRCTMVTLR
                     PEGGKGAEIKKLGPHVMMDDATAGLIETEDARELIEAINEQLGS"
BASE COUNT          511 a          592 c          724 g          449 t
ORIGIN      
        1 gcgggccgga tatgccgaag ccgatccaac ttttgatgtt gccgggatcg attcggcgca
       61 caagcttgcc atactggtca atctcgcgta tggaacaccc gtcaacttca aggacatcta
      121 tacggaaggg attacgagca tcacgccgac cgatattgcc tacgcaaaac agtttgggta
      181 tacgatcaaa tcgcttggta tcgcgaagct cgtggatggg gaaattgagg ctcgggtcca
      241 tcccaccatg cttcccgccg cgtcgcccat cgcccaagtc gaagatgtgt acaatgccat
      301 ccaactcgtc ggcgatgccg tcggtgacgt ggtgctgtat ggtcgagggg cgggatccat
      361 gcccaccgga agtgctgtgg tgagcgatgt gatcgcgatc ggccggaatc tgcttaaagg
      421 cgccgtcggt cgagtcccgg tggcgtcgtt tcagcaggac cagagaaaac cgctccggtt
      481 gagatcgatg gaagagatca gctcgctcta ttacttgcgg tttacggtgg tcgatcgacc
      541 gggcgtattg gcacagatcg caggtgagct aggccgctgt gggatcagca tttcctcaat
      601 ggtgcaacaa ggacgccgcg aagggcagac ggtgccggtg gtcatcaaaa cacataccgc
      661 gaaagagcgc gatgtgcaaa ctgcgctacg tgaaatcaac cggcgtgcgt tcgtctccga
      721 cccgaccatg ctcattcgcg tcgaaggcaa ggatgagtga tcgatgaccc gttggcgcgg
      781 agttattgaa gaataccaaa aattcctccc tgtgaccgag aagacgccgg ttatcagcct
      841 gggagagggg aatactcctc taatccgagc cgccaggttg gcgaaagcga ttgcaccagg
      901 ggtcgaactc tacctgaagt ttgagggggt caacccgacc ggttcgttca aagatcgcgg
      961 gatgacattg gccatctcga aggccgtgga gagcggcgca cgagccgtga tgtgcgcgtc
     1021 gaccggaaac acgtccgcct cggctgccgc ctatggagcc cgtgccgggt tggctgtcta
     1081 tgtgttgatc ccggccggta agatcgcgat gggcaaactc tcccaggcga tgatgcatca
     1141 agcgacggtg attcagatcg aaggcaactt tgatcaggcc ctgactctcg tgaaggactt
     1201 ttcgaccgct atgcacatcg aactcgtgaa ctcggtgaat ccgtttagaa tcgaggggca
     1261 gaagaccgcc gcctttgagg tgtgcgatca actcggcgat gccccggccc ttcatgtcct
     1321 gccggtcgga aatgcgggaa atatcacagc ctattggaaa ggatacaagg agtatcgatc
     1381 ggcgaatcaa atcagccgag tgccccgaat gatgggcttt caagccgctg gtgccgctcc
     1441 gatcgtgttg ggcagggtcg tcgatcaacc gcagactatt gcgacggcca ttcgaatcgg
     1501 caatccggcc agctggacct cggcgctgaa tgcgatagag gaatcggcag gcgcgatcga
     1561 tatggtcacg gatgaagaaa ttctccgggc ttacacagca gtagcgtcca cggaaggagt
     1621 tttttgtgag ccggcttcag ctgcgtccgt cgccggcgtc gccaagctgc atcgaaccaa
     1681 gattctacgg gaaggagaga cagtcgtatg cacgctgaca ggacatggtt tgaaagatgc
     1741 cgatacggcg atcagcgtgt ccaagcaacc ggtcaccgtc aaggcaacgc gcgaggacgt
     1801 tgctcggcta ttgaatgtct gaaagacgtg tggagcttat gaaatatgtg atggtgcatg
     1861 ccggcgggat ggcggaccgt ccgaggccgg aactgggggg acgcacaccg ctgcaagcgt
     1921 catcgactcc caatctcgat cgtctcgcac agatcggaga actcggtcaa ctgctgactc
     1981 ccgaagacgg agttcgtcac gggagcggat tgatcggggc cgcaattctt gggtacgacc
     2041 cgagaaagtt ttatcagggc ccggggccgc tggaagcggc gagtttgggt gtgtcggtga
     2101 cggaacacga tgtggtgtat cgctgtacga tggtcacctt gcgtccggag ggagggaagg
     2161 gcgccgagat caagaaatta gggccgcacg tgatgatgga tgatgcgacg gccgggttga
     2221 tcgaaacgga agacgcgcgc gaattgatcg aagcgatcaa tgaacagctc ggctcg
//
LOCUS       DATMTI010000927         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_842005, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000927 DATMTI010000000
VERSION     DATMTI010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842005"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VN039_14575"
     CDS             <1..359
                     /locus_tag="VN039_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV47224.1"
                     /translation="LLINLASGYLFGSGEDQLKPAGADALQRVGGVLKDFPEKQVHIA
                     GYTDNVSIKGALQKKYASNKELSDARANGAAQALRDGGLTSNLSAAGHGESNPVASNK
                     TAEGRAKNRRVEVIVR"
     gene            complement(498..977)
                     /locus_tag="VN039_14580"
     CDS             complement(498..977)
                     /locus_tag="VN039_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV47225.1"
                     /translation="MSPDHNSRPPFPPFTHETATRKVRMAEDVWNERDPERVSSAYTV
                     DSVWRNRSEFLSGRDAIVAFLRRKWEKELDYRLIKELWAFHGNRIAVRFAYEWHDDSG
                     NWFRSYGNENWEFDGDGLMCRRIASINDLPITESERKYLWALGRRPDDHPSLSDLGF"
     gene            complement(1022..1642)
                     /locus_tag="VN039_14585"
     CDS             complement(1022..1642)
                     /locus_tag="VN039_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWV47226.1"
                     /translation="MAMRYLDLAMTESVHRAQKQYYGRSARITDAPARDPLGEAEIEF
                     IAARDSFYLGTVSETGWPYIQHRGGPRGFLRVLNSTTLAFADYRGNRQLLTTGNLSVY
                     NRVSLFLMDYQHRERLKLLGRARIEDARRHPELVAQLADQTMRSAVERLVFIDVVSFD
                     WNCSKYITPRYSAEEVEGYAGLLKKRIAELEAELHAATTASGFSTT"
     gene            complement(1713..>2066)
                     /locus_tag="VN039_14590"
     CDS             complement(1713..>2066)
                     /locus_tag="VN039_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47227.1"
                     /translation="MSDPKSGSEEALGRVFNALALASECKQKGDEVAVVFNGTGTRWP
                     AELTKLSHPANALYNSVRDVVQGVSCGCAEVFGAKDAVESCGVPLKKDHALAGTAGLL
                     SLRRYVADGWSIVVF"
BASE COUNT          452 a          603 c          563 g          448 t
ORIGIN      
        1 gtcttctgat caacctggct tccggctatc tcttcggttc aggagaagat caattgaaac
       61 ctgccggagc cgacgcgctg caacgggtgg gcggcgtcct caaagacttt ccggaaaagc
      121 aggtgcacat agcagggtac actgacaatg tgtcgatcaa aggagcgtta cagaagaagt
      181 atgcatcgaa taaggaactg tctgatgcgc gtgcgaacgg cgcagcccag gccctgcgag
      241 atggcggact cacaagcaac ctgtccgcgg ccgggcatgg agaaagcaac cccgttgcga
      301 gcaacaagac ggcagagggg cgagccaaga atcgccgggt cgaggtgatc gttcggtaac
      361 aaagaccagc tcctcgttaa tcaaaaggaa ggggctctcc ctatggaagc agagcccctt
      421 cccttgtata gccgcgtcaa tccccagtgc acgccatctc aaatgcgtac tgcactcagc
      481 tggtttcccg ggtgagctta gaaaccgagg tccgaaagag agggatgatc gtctgggcgt
      541 cggcccagag cccagaggta cttccgctct gattccgtaa tgggaaggtc gttgatgctc
      601 gcgatacgtc gacacataag gccgtctccg tcgaattccc agttttcgtt gccataggat
      661 cgaaaccaat ttccagaatc atcgtgccac tcgtaggcga agcgcacggc gatacggttt
      721 ccatggaacg cccatagttc cttgatgagg cggtaatcca gctccttctc ccatttgcgt
      781 cgcagaaatg cgacgatcgc gtctcgtcct gacaggaact ctgaacggtt tcgccatacg
      841 ctatcgaccg tgtaggccga cgacactctc tcgggatcgc gctcattcca cacatcctct
      901 gccatgcgca cttttcgtgt ggcagtttca tgagtgaaag gtggaaaagg ggggcggctg
      961 ttgtgatccg gcgacataag atcctcatgc gggtgcggca atcatagaat gcgacttcat
     1021 gtcaggttgt ggagaaccct gacgccgttg ttgcagcatg caactcggcc tccagttcgg
     1081 cgatgcgctt cttcagcaaa ccggcatacc cttcgacttc ctctgccgag tagcgtggcg
     1141 tgatatattt tgagcaattc caatcaaatg acacgacgtc gatgaagacc agccgctcca
     1201 ccgctgaccg catcgtctgg tcagcgagct gcgcaacgag ttccggatgg cggcgggcgt
     1261 cttcgatgcg agcccggcca agaagtttca gccgttcacg atgctgataa tccatgagga
     1321 acaacgaaac ccggttgtac accgagagat tgcccgtggt taagagttgc cgattgcctc
     1381 ggtaatcggc aaacgccagg gttgtcgaat tgaggacgcg caaaaatcct ctcggcccac
     1441 cacgatgttg gatatagggc catccggttt cgctcaccgt acccagatag aaactatccc
     1501 ttgccgcgat gaactcgatc tccgcttcgc cgagtggatc gcgtgccggc gcgtcggtga
     1561 ttcttgccga gcggccataa tattgtttct gtgcacggtg taccgattcc gtcatcgcca
     1621 gatctaaata tctcatcgcc atcgtctcat cctccgtcac atcgccgtac gaggcttttt
     1681 ggcttacacg tcggtatgcc gcgttgtctg tatcagaaca ccacgatgct ccatccatcc
     1741 gccacgtacc ggcgcagact caacagtccc gctgtcccgg ccagcgcatg atccttcttg
     1801 agcggcactc cacatgattc gaccgcgtcc tttgctccaa acacctcagc acaaccgcat
     1861 gagacgcctt gcaccacatc gcgaaccgaa ttgtagaggg cgttggccgg atgcgagagc
     1921 tttgtgagtt cggcgggcca acgggttccg gtcccgttga acaccacggc aacttcatcg
     1981 cccttttgct tgcattcgga tgcaagtgcg agcgcgttga agactctccc gagcgcctct
     2041 tctgaaccgc tctttggatc ggacat
//
LOCUS       DATMTI010000928         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_416759, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000928 DATMTI010000000
VERSION     DATMTI010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416759"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..544
                     /locus_tag="VN039_14595"
     CDS             80..544
                     /locus_tag="VN039_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47228.1"
                     /translation="MDMRDAAARAVIFQIVEQEAKKLKDEAKAELAQMEPGDTLAGKW
                     GDQLLGKATMTSGRTKLVVTDEEKLITWLQYNHPEEILLSPNPAYMRQIEERARKLGV
                     PVDGEGEAVPAVSLEQGEPYVSVRKTPDAPFVVAELLSRGRVQLDGIKELEQ"
     gene            544..939
                     /locus_tag="VN039_14600"
     CDS             544..939
                     /locus_tag="VN039_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47229.1"
                     /translation="MGGFNLDNYNDVPSRMQEFFNKYPDGSLQGSYEFKQIPMYLKDK
                     ETEMPVLAGEKTIVIYTAKAYRSPEDQLPGIGTASEPFPGLTPYTKDSEMMNAETSAW
                     GRAILAVGAADTRKGIASREEVQSRKGDW"
     gene            1637..>1924
                     /locus_tag="VN039_14605"
     CDS             1637..>1924
                     /locus_tag="VN039_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47230.1"
                     /translation="MTGFSREVRQLVCDRANADYDSDYIVCEVMVRCQGRATAQYDLH
                     HRRNRGSGGSKRLDTNLAGNCLAACRDCHNFITTHPELGLRNGWLVRQNQTP"
BASE COUNT          441 a          500 c          611 g          372 t
ORIGIN      
        1 ggactacgga tgaaacctac ttccacggcc tccttttaga agccaggaaa acgttgaccg
       61 agattttgca ggagttttga tggacatgag agacgcagct gcccgcgctg tcatatttca
      121 gattgttgag caggaagcga agaagctgaa agacgaagcc aaggctgaac tcgcccagat
      181 ggaaccgggg gacactcttg ccggcaagtg gggggaccag ttgttgggta aggccacgat
      241 gacgtctggg cgtacgaaac ttgttgtgac cgatgaagag aagctgatta cgtggttgca
      301 gtacaaccat cccgaagaga tcctgttgtc gccgaatccg gcgtatatgc ggcagatcga
      361 ggaacgcgct aggaagctcg gtgttccggt tgacggcgaa ggcgaagctg taccggccgt
      421 ctcccttgaa caaggcgaac cgtacgtgtc ggtgcgcaag acaccagatg ccccgttcgt
      481 ggtcgctgaa ttgttgagca ggggacgggt ccagctcgac ggcatcaaag aactggagca
      541 gtgatgggcg gcttcaatct ggacaactac aacgatgtcc cctcacggat gcaggagttc
      601 ttcaacaaat accccgacgg ctcactccaa ggctcctacg agttcaagca gataccaatg
      661 tatttgaagg acaaggaaac tgagatgccg gtgttggcgg gggagaagac gattgtcatc
      721 tacaccgcga aagcctaccg gtcaccggag gaccaattac ctggtatcgg gacggcgtcg
      781 gagcctttcc ctgggttgac gccgtacacg aaggattcgg agatgatgaa cgccgaaacc
      841 tccgcgtggg ggagagcgat tctggctgtg ggggctgcgg atacccgtaa aggtattgct
      901 tctcgtgagg aagttcagtc acgcaagggg gactggtgac cggattctcc cctcaggtga
      961 agcagttgat gcgggatcgg gcgttgatgg acggcctgat ccgccgctgc actgtcgaag
     1021 gttgcgaacg ccagctgcgc gctcgcggat actgcacgac tcattgggct cgctggcgta
     1081 agtacggaac tccagaattg ccgacgtttg cgacggtgtg cgaagtcgaa cactgcgacc
     1141 gcccagtcag gtcaaagcgt gccggctact gcgagatgca ctatgggcgg ctccgtcggg
     1201 gtcgcggact aggggagcca ctacaggtac aaggacagtg tctggcggac gggtgtgagc
     1261 gggacgcgga ccactcgggt atgggtaacc gaaaaacaac ggaagggtac tgccgcctcc
     1321 attatctgcg tctgcgtaat cacggcgacc tgtaccggga gaatcggggc gacaaccacc
     1381 ctctatggac aggtgacgaa gccacctaca cggctgtaca ccagcgtgtg aagtgcaacc
     1441 gtggcgttcc gtcaacacat ctgtgtgtgg attgcgggcg caccgctagg cactggtctt
     1501 acgaccgaac ggacccagat cctcggtttc aagagggaaa aggtccgtac agcttggaca
     1561 tcaaccgcta tgtgccgcgt tgcgtgaagt gccacaagaa ttttgacatg aaatttgtga
     1621 tggcggagcg tgcccgatga ctggattctc aagggaagtt cgacaactcg tctgcgaccg
     1681 cgcgaacgcc gattacgact ctgactacat cgtgtgtgag gtgatggtcc gctgccaggg
     1741 ccgcgcgaca gcacagtacg acctacacca tcgccgtaac agggggagcg ggggcagtaa
     1801 gcgcctggac acgaacctgg ctggcaactg cctcgcggct tgccgtgatt gtcataactt
     1861 catcacgact catccggagt tggggttgcg gaatggttgg ttggttcgtc aaaaccaaac
     1921 cccc
//
LOCUS       DATMTI010000929         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_205448, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000929 DATMTI010000000
VERSION     DATMTI010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205448"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VN039_14610"
     CDS             complement(<1..745)
                     /locus_tag="VN039_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HWV47231.1"
                     /translation="MPEPVQLTKFGVTELEAQHALDRLNVRDVDYADLYFESRTSESV
                     SMEEGIVKRAAKSVSQGVGVRATAGEKTGFAYSDELTKKDLEIAADTARYIANSPRGD
                     LPVPVPTQRRPTRDLYPIERAKAEVSTADRVALLNEIDAEARRYDPRIKNVMASFNTE
                     YKVVAVATSDGTLVGDIQPLSRLQITCIAEDRDNRQIGSFGGGGRVGFEYYREDNRHL
                     SYAREAAREAILNLSAVDAPAGVMPVVLGG"
     gene            946..1731
                     /locus_tag="VN039_14615"
     CDS             946..1731
                     /locus_tag="VN039_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HWV47232.1"
                     /translation="MTHHPTSSFDHLSESELTAKLELELLPKHVAVIMDGNGRWAELR
                     GLPRIAGHREGINSVREMITLCLELGIRALTIYAFSQENWNRPTQEISALMGLLEHYL
                     STERASLIEQGVCFRAIGRHELLPPSAQHWVRTTEKETAHLDKLTLTVALSYGGRAEI
                     VDAVKTLIEDVQAGTVQSALIDETTIQQYLYTHPLQDPDLLIRTSGETRISNFLLWQL
                     AYTELCFTPTLWPDFRRREFLLALIEYQRRERRFGRVLSTLSS"
     gene            1784..>2386
                     /locus_tag="VN039_14620"
     CDS             1784..>2386
                     /locus_tag="VN039_14620"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWV47233.1"
                     /translation="MTTPPARTRRFDLRRLYTAAVLIPAVYGIIAYLAPWALTLLLIS
                     VGSIALVEFYQICFQPRFNRLAVGVGLGAFVLVIARDHLSLALTHLVVASVLVVSIAV
                     SISSGRTDPRWKGILITLFGVLYIGFPLSTVVSTRSLPAGEFLVLFLAVVTWTSDTGA
                     YYAGTLWGKHPLLPSVSPKKTVEGLLGGIALAVGTALLAQQ"
BASE COUNT          482 a          729 c          619 g          556 t
ORIGIN      
        1 cgccgcccaa cacaacaggc atcactccgg ccggagcatc caccgccgac aagttaagga
       61 tcgcctctct cgcggcttct cgcgcatagc tcaaatgtcg attgtcctcg cggtagtact
      121 cgaatcccac ccggcctcct ccaccgaaac tcccgatctg tcgattgtca cggtcttcgg
      181 caatacaagt gatctgcaga cgggacaacg gttgaatgtc gccgaccagc gtcccatcag
      241 aggtcgcaac agccaccacc ttgtattcgg tgttaaaaga agccatcacg ttcttaatgc
      301 gggggtcgta ccgccgggct tcggcatcga tttcattcaa tagcgccaca cgatcggccg
      361 tcgacacttc ggccttcgcc cgttcgatcg gatacaggtc tctggtcggt cgacgctggg
      421 taggcaccgg aacgggtaaa tctcccctgg gggaattggc gatgtagcgt gccgtatcgg
      481 ccgcaatctc caaatccttc ttcgtgagtt cgtccgagta ggcaaaccct gttttttcgc
      541 cggcggtcgc gcggaccccg accccttgag agacgctctt cgccgcacgt ttaacgatgc
      601 cttcctccat cgaaaccgat tccgaggtac gggactcgaa atagagatcc gcataatcaa
      661 catccctcac gttcagccga tcgagcgcat gctgggcttc gagttcggtg acaccgaatt
      721 tggttagctg aaccggctcg ggcatagtat ggttgcctcc tcgtcccttc ggctgatgag
      781 cgagtatagc ccattcgttt ccgccgtcgc aatgcgatga ttctccactc agcggattga
      841 tgaagcgttt ccgagttatc gtttgacatt ccataccccc gacagtagac tttcccgact
      901 tctctgcctc tcacgccgga catgaggagc gcgctctaag ctcgtatgac acaccacccg
      961 acttcaagtt tcgaccatct gtccgagagc gaattgaccg ccaagttgga actggaactg
     1021 ctcccgaaac atgtcgcggt catcatggat ggcaacggcc ggtgggcgga actccgcggt
     1081 ctccctcgta ttgcaggcca tcgtgaaggg atcaattccg tcagagagat gatcaccctc
     1141 tgcttggaac tcggcatccg tgctctgacc atctatgcat tttcacaaga aaattggaac
     1201 cgtccgacac aagaaatcag cgcgctcatg ggactcctgg aacattatct ttctacggaa
     1261 cgtgccagct tgatcgaaca aggggtttgt ttccgcgcca tcggccgcca tgagctgctc
     1321 cccccatcgg cccaacattg ggttcgcacg accgaaaaag aaacggcgca cctcgataag
     1381 ctgaccctga cggtcgccct cagctatgga ggacgagcgg aaatcgtcga cgcagtgaaa
     1441 actctgatag aggacgtcca ggcgggaact gtacaatctg cccttatcga tgaaaccacg
     1501 atccagcagt acctctacac gcacccgctc caggatccgg acttactgat tcgtacgagt
     1561 ggagaaacgc ggatcagcaa ctttctcctg tggcagctgg cctacacgga gttatgcttc
     1621 acgccgacct tgtggccgga ttttcggcga cgggagttcc tgctcgcgct gatcgagtat
     1681 caaagacggg agcgccgctt tggccgagtg ttgagcaccc tgtcttcgta accttgtggg
     1741 atatccaccc accaccgaag cgattgtcca cagctggata ttcgtgacca ccccaccagc
     1801 acgcactaga cggttcgatc tccggcgtct ctatacggca gcggtgctta tcccggcagt
     1861 ctatggcatc atcgcatacc ttgctccatg ggccttgacg ctactattga tctccgtagg
     1921 gtccatcgcg ttagtagagt tctatcaaat ctgttttcaa cctcgtttca accgtctcgc
     1981 ggtcggagtc ggacttggag cgtttgtcct ggtcatagcc cgtgatcatt tgtctctcgc
     2041 cttgacacat cttgtggtcg cctcggttct cgtggtgtcg atcgctgttt ccatttcatc
     2101 cggtcggact gatccccggt ggaaaggtat actgattacc ctcttcgggg tgctgtatat
     2161 cggatttcct ctcagcacag tggtatcgac ccgttccttg cctgccggcg aattcctcgt
     2221 cctgtttctg gcggtggtca catggacatc ggacaccggc gcctactacg ccgggactct
     2281 gtggggcaaa catcctctcc tgccgtcggt cagccccaag aagactgtcg aggggttact
     2341 cggaggaatc gcactggccg tcggtaccgc attattggct cagcag
//
LOCUS       DATMTI010000930         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_416843, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000930 DATMTI010000000
VERSION     DATMTI010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416843"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /gene="pnp"
                     /locus_tag="VN039_14625"
     CDS             <1..1880
                     /gene="pnp"
                     /locus_tag="VN039_14625"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWV47234.1"
                     /translation="RPTEKEVLTSRLIDRPIRPLFPEGYYYETQVIASVLSADKTGSS
                     DVIGITAASAALAVSNIPFDGPVAGVKIGRVNGQLVVNPDLETMEQSELHLVVAGTAD
                     AVMMVEAGANELSEQTMLEALELAHAEIKKIVQKIEELAKKVGRVKREVLAESIDSAL
                     QAEIKTLVAQPIRDAIMIANKTARQERLDQILADAIEKLKKPEDPSSERHVKIVFHQL
                     EYTEVRKMILENRSRADGRGPTDIRPITCEVGALPRAHGSAIFTRGETQSLAVVTLGT
                     TDDEQRIDALEGEYTRTFMLHYNFPPFSVGEARPLRSPGRREVGHGALAERALKPVIP
                     NKDVFPYTLRIVSEILESNGSSSMATVCGGTLAMMDAGVPIKEPVAGIAMGLIKEGDD
                     VIILSDILGLEDHLGDMDFKVCGTKNGITALQMDIKIGGITTALMQQALEQARLGRLH
                     ILDRMSKGLAGPRTDLSPFAPRIYTMKVKQDKIRDIIGQGGKTIRGIQSDCGVKISVE
                     DTGIVTIASADGASLQKAKEIINRLTEEVEVGKVYMGTVRKIMDFGAFVEVLPGTDGL
                     VHISQLAHHRVKAVSDEVAEGDQILVKVLEIDKQGKIRLSRKETIPAPARNGANDSAG
                     G"
     gene            1884..>3039
                     /locus_tag="VN039_14630"
     CDS             1884..>3039
                     /locus_tag="VN039_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWV47235.1"
                     /translation="MYRKLVLDNGIRLVTEQIPTLKSVTVGIWVNAGSRDESPAEAGY
                     SHFVEHMFFKGTGTRSATDISREIDALGGEMNAFTTRETTTFYVKVLDQHLPKALDLL
                     SDLFLRSRLGRKEIEKEKQVVLEEIRMVQDDPEDLVQELHAKVVMGRHPLSRPILGRE
                     STIIRISRQNLLEYIDSHYRPEEIVVAIAGNFDHRQLEKTMTRTFGGYRKSSSGIPRK
                     RWPPELLGGAMMKQKSLEQVHLCVGFESVAAGHKDRYAVYALNAVLGGSVSSRLFQEI
                     REKRGLSYSIYSFLSGYSDSGTITVYAGIRAREVERVLDLVYREIRKLARHGIDGHEL
                     KRTKDQMKGSLMLSLESSHSRMNKLAKDELIAGTHTTLEDLIAEIDGVTEQ"
BASE COUNT          726 a          761 c          914 g          638 t
ORIGIN      
        1 gtcgaccgac tgaaaaggaa gtcctgacaa gccgcttgat tgaccgtccg atccgcccat
       61 tgttccccga agggtactat tacgagacac aggtcattgc ctcggttctg tcggcagata
      121 agaccggttc ttccgacgtc atcggaatta ctgcggcatc agccgccctc gctgtgtcga
      181 acatcccttt cgacggtcct gttgccgggg tgaagatcgg tcgcgtcaac ggccaactcg
      241 tcgtcaatcc tgacctcgaa acgatggagc agagcgaact gcatctggtg gttgcaggta
      301 cagcggatgc ggtgatgatg gtagaggcag gcgccaatga attatccgag cagacgatgc
      361 tcgaggcgct ggaattggct cacgctgaga ttaagaaaat cgtccagaag atcgaggaat
      421 tggccaagaa ggtcggtcgg gtgaagcggg aggttcttgc ggagtcgatc gactccgcat
      481 tgcaagccga gatcaagacg ttggttgcac aaccgattcg tgacgccatc atgatcgcca
      541 acaagaccgc cagacaggaa cggttggatc aaattcttgc cgatgctatt gaaaaactga
      601 agaagcctga ggatccctca agcgaacggc acgtcaagat tgtgttccat caattggaat
      661 acacggaagt ccggaagatg atcttagaga accgatcgcg ggccgacgga cgagggccga
      721 cggatatcag gccgatcacc tgcgaggtcg gtgccttgcc tcgcgcacat ggttccgcaa
      781 tctttacccg aggcgaaact cagagcttgg cggtggtgac gctggggacg accgacgatg
      841 agcagcgcat tgacgctttg gaaggcgagt atacccggac atttatgctg cattacaact
      901 tcccgccctt cagcgtcggc gaggcgcggc cgctccgctc accgggaagg cgtgaggttg
      961 gacacggagc cttggccgaa cgggcattga agcctgtcat tccaaacaaa gatgtcttcc
     1021 cgtatacctt gcggattgtt tctgaaattc tggagtcaaa tgggtcttct tcgatggcaa
     1081 ccgtatgcgg gggaacgctg gccatgatgg acgccggagt ccctattaaa gagccggtcg
     1141 cgggcatcgc aatgggattg atcaaagaag gggacgacgt cattatcttg tcggacatcc
     1201 ttggccttga agatcatctg ggcgatatgg acttcaaggt ctgcgggacc aagaacggga
     1261 ttacggcgct tcaaatggac atcaagatcg gaggcattac cacggcgttg atgcagcaag
     1321 ccttggagca ggcgcggttg ggacgcctcc acattctcga ccgcatgtcc aaggggcttg
     1381 cgggcccgcg caccgacttg tcgccgttcg cgccccgcat ctacacgatg aaggtcaagc
     1441 aggataagat tcgagatatc atcgggcagg gcggcaagac catccgcggg attcagtccg
     1501 actgcggagt gaagatcagt gtcgaagata ccggtattgt gaccatcgct tctgcggatg
     1561 gcgcgtcgtt acaaaaagcc aaggagatca tcaaccgctt gactgaggaa gtcgaagtcg
     1621 ggaaggtcta catgggtacg gtaagaaaga ttatggactt tggtgcgttc gtggaggtgt
     1681 tgccgggcac ggatgggttg gtgcacatct cgcagcttgc gcatcaccga gtgaaagccg
     1741 tatccgatga ggtggccgaa ggcgaccaaa ttctggtgaa agtgctggag atcgacaaac
     1801 aaggcaagat caggctcagt cgaaaagaga ccattcctgc accggcaaga aacggtgcca
     1861 acgattcagc gggcgggtaa cacttgtacc gcaagctcgt tctcgataac ggaattcggt
     1921 tggttactga gcagattcca acccttaagt ctgtgacggt tggtatctgg gtcaatgcgg
     1981 gctctcgtga tgaaagtccc gcagaggccg ggtattcgca cttcgttgaa cacatgttct
     2041 tcaaagggac gggtactcgt tcggcgacgg acatctcccg tgaaatcgac gcgttggggg
     2101 gcgagatgaa cgccttcacg acacgcgaaa cgacgacgtt ctacgtcaag gtcttggatc
     2161 agcacttgcc caaagcgttg gacctgcttt cggatttgtt tcttcgctct cgtcttggcc
     2221 ggaaagaaat tgagaaagaa aaacaggtgg tgctcgaaga aatccgcatg gttcaagatg
     2281 accctgaaga tctggtccag gaacttcatg ccaaggtggt catggggcgc catcccttga
     2341 gtcgaccgat cttggggcga gaatcaacca tcatccggat cagccggcag aatcttcttg
     2401 agtatatcga ctcccattac cgcccggaag aaatcgtggt ggcgatcgcg ggaaatttcg
     2461 atcaccgtca gcttgaaaaa acaatgactc gcaccttcgg cgggtatcgt aagtcctcaa
     2521 gcggcattcc tcgcaaacgt tggcctcctg aattgctggg gggcgcgatg atgaaacaga
     2581 agtcactgga acaggttcat ctctgcgtgg ggtttgagag cgttgccgcc gggcataagg
     2641 accgctatgc ggtctatgcg ctgaacgcgg tgctaggcgg cagtgtcagc tcgaggctct
     2701 tccaggagat ccgagaaaaa cgcggtttgt cctattcaat ctactcgttt ttgtccggct
     2761 attcagacag cggcacaatt acggtctacg ccgggattcg cgcacgagag gttgagcggg
     2821 ttctcgatct ggtctaccgc gaaattcgaa aactggccag acatggtatc gatggtcacg
     2881 agctgaaacg cacgaaggat caaatgaagg gtagcctcat gctgagcttg gagagctcac
     2941 atagtcggat gaacaagctt gccaaggacg agctgattgc cgggacacac acgaccctgg
     3001 aggatttgat tgcggagatc gatggcgtaa ctgagcaac
//
LOCUS       DATMTI010000931         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_670850, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000931 DATMTI010000000
VERSION     DATMTI010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670850"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..308)
                     /gene="rrf"
                     /locus_tag="VN039_14635"
     rRNA            complement(194..308)
                     /gene="rrf"
                     /locus_tag="VN039_14635"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(496..>3078)
                     /locus_tag="VN039_14640"
     rRNA            complement(496..>3078)
                     /locus_tag="VN039_14640"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          573 a          975 c          780 g          750 t
ORIGIN      
        1 cgggcgggag attgcttcgc tccgctcgca atgacgccgg ctccgctcgc aatgacgccg
       61 gctcccctcg ccatgacgcc gcggcgccga accagtcagc aggccggccg gcatgttgtg
      121 acgctctgcc cgactgatcc ctgacactga tccctggcac tgatccacgg ccccttgtca
      181 gacaggctgg aagacctggc ggcgacctac tctcccgtgg cttgggccac agtaccatgg
      241 gcgctggggg ttttcacggc cgagttcggg atgggatcgg gtgcggcccc cccgccaagg
      301 ccaccaggtc gtccagcctg tccggaacag gtttcagtgg tcggcggtca gtggtcagtc
      361 aagcgaccac gggcagccga caggttctgg tgcgggtgcg tatgatgtgt gctgtcactg
      421 cggatgattt ctgtgcatgg agggtccgcc gccacgatca gcggactgac cgctgaccac
      481 tgaccgctga ccactggtct cgagccgatc gggcgattag gaccggtcgg ctgcacgcgt
      541 tgccgcgcgt ccacctccgg cctatcgacg tgctggtctt gcacggccct tatggggaga
      601 cctggtttag aggagggttt cccgcttaga tgctttcagc ggttatcccg tccgcacata
      661 gctacccggc ggtgccgctg gcgcgacaac cggtgcacca gaggtgcgtc catcccggtc
      721 ctctcgtact agggacagcg cctcgcaagt ctccgtcacc cacggcagat agggaccgaa
      781 ctgtctcacg acgttctaaa cccagctcac gtaccacttt aatcggcgaa cagccgaacc
      841 cttgggacct gctccagccc caggatgtga tgagccgaca tcgaggtgcc aaacctcccc
      901 gtcgatatgg actcttgggg gagatcagcc tgttatcccc ggcgtacctt ttatccgttg
      961 agcgatggcc cttccacgtg gaaccaccgg atcactatga ccgactttcg tctctgctcg
     1021 acatgtacgt ctcgcagtca ggcaggctta tgccattgca ctcgacagct gatttccgac
     1081 cagcctgagc ctaccatcgc gcgcctccgt tactctttgg gaggcgaccg ccccagtcaa
     1141 actacccacc atgcagggtc ccggatccgg ataacggacc gcggttagac atcagtaaaa
     1201 gtaagggtgg tatttcaagg acggctccat ccgagctggc gcccggactt caaagcctac
     1261 cacctatcct acacatgccg tcactaatgc cactgcaaag ctatagtaaa ggtgcacggg
     1321 gtctttccgt ctgaccgcag gaaccccgca tcttcacggg gaattcaatt tcactgagtc
     1381 gatgctggag acagcgggga agtcgttacg ccattcgtgc aggtcggaac ttacccgaca
     1441 aggaatttcg ctaccttagg accgttattg ttacggccgc cgtttaccgg ggcttcgatt
     1501 caaagcttgc acctctcctc ttaaccttcc ggcaccgggc aggcgtcaga cgctatacgt
     1561 catctttgga tttcgcagcg ccctgtgttt ttagtaaaca gtcgccaccc cctgctatgt
     1621 gcccccggcc tctacttgcg tagaaaccgg gccctcttct tccgaagtta cgagggcaat
     1681 ttgcctagtt ccttcaacac cgttctctca agcgccttag catactctgc cagtccacct
     1741 gtgtcggttt ggggtacggt ctgaacgtgg gagctatttc ctgggacctc ttcgcggccc
     1801 gctcaatcca gtaagagcga acaacttaca tcatccgtca ctacccacag gcccaggaat
     1861 gttcacctgg ttcccatcga ctacgccttt cggccttgcc ttaggggccg gctaaccctg
     1921 cgcagattaa cttgacgcag gaacccttgg acttacggcg agagggtctc tcaccctctt
     1981 tatcgctact catgtcagca ttcttacttc cgatatctcc agagtcgctc acgcttctcc
     2041 ttcactgaca tacggaacac tctgctacca ttccaccttg cggtgaaatc ctaagcttcg
     2101 gtacgttact tgagccccgt tacattttcg gcgcaaagtt ccttgtttag accagtgagc
     2161 tgttacgctt tctttaaatg atggctgctt ctaagccaac atcctggttg ttttgggaac
     2221 ctcacatcct ttcacactta gtcacgattt ggggacctta gctgtaggtc agggtttttt
     2281 ccctcttcac gacggacgtt agcacccgcc gtgtgtctgc catgctcagc actcaccggt
     2341 attcggagtt tggttaggtt tggtaagcgg tgaagccccc tagcccatcc agtgctctac
     2401 ccccggtggt gatacatgac gcgctaccta aatagctttc gcagagaacc agctatctgc
     2461 aagtttgatt ggcctttcac ccctagccac aactcatccc cgactttttc aacaggcgtg
     2521 ggttcggtcc tccagtgcgt gttaccgcac cttcaacctg gtcatggcta gatcacttgc
     2581 tttcgggtct catccgtcta actcaatcgc cctattcaga ctcgctttcg ctgcgcctcc
     2641 gccttgtggc ttaagcttgc tagacagacg aactcgctga cccattatac aaaaggtacg
     2701 aggtcactcc gaagagctcc ctctgtttgt aggcgtccgg tttcaggtct ctttcactcc
     2761 cctcgacggg gtgcttttca cctttccctc acggtactag ttcgctatcg gtcacacacg
     2821 agtacttagg cttggaacgt ggtcgtccca tgttcagaca gggtttcacg tgccccgtcc
     2881 tactcgagga ccttcgaaat ttttacccgt acggggctat cacccgctat ggccactctt
     2941 tccagagtgt tccggttatt aatcgaaggc cactggcctg atccgctttc gctcgccact
     3001 actaacggag tctctgttga tgtcctttcc tacaggtact gagatgtttc agttccctgc
     3061 gttcgctcct ccaaccta
//
LOCUS       DATMTI010000932         1637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_544494, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000932 DATMTI010000000
VERSION     DATMTI010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544494"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..196)
                     /gene="tatA"
                     /locus_tag="VN039_14645"
                     /pseudo
     CDS             complement(68..196)
                     /gene="tatA"
                     /locus_tag="VN039_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(312..1118)
                     /locus_tag="VN039_14650"
     CDS             complement(312..1118)
                     /locus_tag="VN039_14650"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV47236.1"
                     /translation="MENPEPTSSQSETVLDPGDQPLTPDEEISEIEKLLAGEPDDFQA
                     RCRLGELYFSKGRLDDASAEVKKAIEMAESLRTEMNRSLAMYYSNLGTIYATKNMADE
                     AETEFRHALDIFPHDVLALFNLGRLYADKKQYMEAKSYYERLVEITPDDPIAWYNLAG
                     VYIELDNPQVSDYNTIDVGIQCYLRTLELDPKHLESSFKLMEIALNHKKTDLAVRVME
                     SAVEHNPDEPLAYYNLISVYDKCKMFDQAEETRKRLKERFAKKPKDGPKS"
     gene            complement(1137..>1637)
                     /locus_tag="VN039_14655"
     CDS             complement(1137..>1637)
                     /locus_tag="VN039_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47237.1"
                     /translation="FVFVAYGKRKSLSLRTKNRRQRNRAMWFWSTTGIIFSALLVADY
                     VLDLYNPALASAVRQASQGIGWIFQFGMGYTFYLPFAVYVGGLLCWSYTVVKLLMIGR
                     LAGYGIGLMFIAGYALLFSNLTLMVVLGVMLLALDRVRPTAVESVPASAGPVMPESNG
                     LLPGQA"
BASE COUNT          382 a          472 c          403 g          380 t
ORIGIN      
        1 tttgaggtgc agcggcctgt tgccccgccg cctgagcctc ggcctcgatg gcgtccgcct
       61 catggacgga cttcttgaat cccttgatcg ccttgccgac cccctcaccc aattgcggca
      121 atttcccggc gccgaagata atcagcacaa tcatgagtat aagaatcagc tcggtgaacc
      181 ccaaactgcc gaacataatg cacctctttt tgaaagtgga gcctcacggc tttgccggcc
      241 gtggcccctg tttggttctt cggcgacact ctcgccgaag cctattctcc tatacttgca
      301 tagcgggtaa attaggactt tggaccatcc ttcggcttct tggcaaaccg ctcttttaac
      361 cgtttgcggg tctcttccgc ctgatcaaac atcttacact tgtcgtacac gctgatgagg
      421 ttgtaatacg cgagcggttc atccggattg tgctccaccg cactctccat gactctgacc
      481 gccaaatcgg tcttcttatg attgagcgcg atttccatca gtttgaaact tgattccaag
      541 tgtttgggat ccagctccaa ggtgcgcagg tagcactgaa tgcccacgtc gatcgtgttg
      601 tagtcggaca cttgagggtt atccaactca atatagaccc ctgcaaggtt ataccaagcg
      661 atggggtcgt caggagtgat ttcgacgagg cgctcatagt agctcttcgc ctccatatat
      721 tgctttttgt ctgcatagag cctcccaagg ttgaacaacg ccaggacatc atgggggaag
      781 atatccagcg catgccggaa ctcggtctcg gcttcatcgg ccatgttctt ggtggcatag
      841 atcgttccca gatttgagta atacatggcc agcgagcgat tcatttccgt gcgaagagat
      901 tccgccatct caatcgcttt cttgacttct gccgaggcat catcaagccg ccctttactg
      961 aaatacaatt caccgagtcg acagcgagcc tgaaagtcat ccggttcacc ggccaacaat
     1021 ttttcgatct cggagatctc ctcgtcggga gtgagcggct gatcgccggg atcaagaacg
     1081 gtctctgatt gcgatgacgt cggttctgga ttttccataa tcatctcgct ccgaaactaa
     1141 gcctgcccgg ggagtaggcc gttcgattcc ggcatcacag ggcctgcgga agccggcacc
     1201 gattcaacgg cggttggtct cactcgatca agggcaagca acatgactcc gagaacaacc
     1261 atcaacgtaa ggttcgaaaa caataaggca taaccggcga taaacatcag accgatcccg
     1321 tagccggcca gtcgcccgat catcagcaac ttgacaacgg tgtaggacca gcagagcaat
     1381 cccccgacat acaccgcaaa ggggagatag aaggtatagc ccattccgaa ttgaaagatc
     1441 cacccgatgc cctgcgaggc ctgccggaca gcagaagcca atgccggatt atacaggtcc
     1501 aagacgtagt ctgcgacaag gagagctgaa aaaatgatcc ccgtcgtcga ccaaaaccac
     1561 atggctcggt tccgctgccg tcgattcttc gttcgcagcg atagactctt cctcttgccg
     1621 taggccacaa acacgaa
//
LOCUS       DATMTI010000933         8746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_544521, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000933 DATMTI010000000
VERSION     DATMTI010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544521"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(662..2215)
                     /locus_tag="VN039_14660"
     CDS             complement(662..2215)
                     /locus_tag="VN039_14660"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWV47238.1"
                     /translation="MHPVAPRDTITLAGLHSLLAVRTILVCAGLFTAWPAWGLTAGDL
                     YRSGKEATALVIGIDDEAHALSFGSGVFISQDGLLLTNAHVIHEATRIVVYGAADSLT
                     PTPEIVAVDPDLDLAALRIPYAHPVPYLSLASKPAEDGMAAVTIGYPRLPDVLQMGLT
                     IHPTVFPVTVSGEAMGQSRIAHRPLPFLHITGLMNAGSSGGPLVAAASGHILGLVVHT
                     VPYLETAKNTQGESIGRVMLRAGLSYCIPSSRIREWLDADHLLSGGVNEPPGQTQPAM
                     PKPTASDGFVTTAHLLQLMADVIRRDPDLLDMAIQHYESALLLESDRPDILHDLGRAL
                     ASKGNVNDAWARYEAALARLPDDLELLTDAGDLRRQQRQFDKAETFYRQALQRDSCYS
                     RALLGLGNTMAEQGDSQEARRAFQAVAHCPSASSLAAYRLGIELERHGESTGALTLWE
                     HALEQLSNQVPAEQGLITRIRHRANQLRNRLQRPSAQTADDFHLSMAGAGGIKDNHPI
                     NTSRSEQTQ"
     gene            complement(2382..2801)
                     /locus_tag="VN039_14665"
     CDS             complement(2382..2801)
                     /locus_tag="VN039_14665"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HWV47239.1"
                     /translation="MAKTLIEPTSTSADDLRAMLLERRSAVQQEIHELLTQYREHYTA
                     THNHSVLDLEDMSLRDSTAEQQLSLLEARNRTRMMLDTALRRLDDGEYGLCEDCGAKI
                     GAGRLRALPFAKRCLSCQHQAEVLEKIARQEDRRDDA"
     gene            3158..3817
                     /locus_tag="VN039_14670"
     CDS             3158..3817
                     /locus_tag="VN039_14670"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWV47240.1"
                     /translation="MRLTVATYNVHRCIGWDGRYGRERILAVLQELQADLIALQEVDE
                     ENTLQWLAVHLSMTAIAGPNLRRHGAYGNGLLTTCKLGEFRLVDLSFRGHEPRGAIDV
                     DVVCGNERVQVIATHLGLRPSERRWQVRQLLTRFGTRHCILLGDINEWFLWGRPLRWI
                     TKMFGRTPSLRSFPSPWPIFPLDRIWIRPPGSVIEIRTHKTALSSVASDHLPVKAIIE
                     L"
     gene            complement(3820..4446)
                     /locus_tag="VN039_14675"
     CDS             complement(3820..4446)
                     /locus_tag="VN039_14675"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HWV47241.1"
                     /translation="MPTATLQRKYEPRSYDLHDLHGISDRTLAMHFKLYEGYVKETNN
                     LTARIHQLVEDGKVDQEEMPVYSELKRRYGFEYNGMVLHEYYFDNLMANGSPDPGAHS
                     SFRRAAESSFGTYEAWKGDFIGVGKMRGVGWAVCYLNPYNGKLTNHWITLHETGNVAG
                     FAPVLVMDVWEHAFILDYKPSERVSYIEAFFSNINWKAVEHRLERTIP"
     gene            complement(4515..4802)
                     /locus_tag="VN039_14680"
     CDS             complement(4515..4802)
                     /locus_tag="VN039_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47242.1"
                     /translation="MVPTYWEALRIGWTILWRGVGSVVLLLMLVNGLIIIGMPELSRT
                     SPSWGAAFYPFALASLIGAFGIMPWLVKCLCTISYPGFRLRLVHESSASQT"
     gene            complement(4823..5053)
                     /locus_tag="VN039_14685"
     CDS             complement(4823..5053)
                     /locus_tag="VN039_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47243.1"
                     /translation="MLKAFAINLLLVAYLVSASMQAAILVRPHEASPPTDPSPGPTDP
                     TPKPSGPIPPPQPPPPKPNPVHPSPSPGLDST"
     gene            complement(5100..5474)
                     /locus_tag="VN039_14690"
     CDS             complement(5100..5474)
                     /locus_tag="VN039_14690"
                     /inference="COORDINATES: protein motif:HMM:NF015798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HWV47244.1"
                     /translation="MEMKTLSQQHQNCLNACFSCAQTCEACANDMIGMEHHDHNDLMT
                     RCIRLCRDCADICVLAAKFISRSSQQAESLCRLCAEICDECAEVCERHAPQHALCGPC
                     AKECRRCADLCREMVGTVVPSA"
     gene            complement(5539..6063)
                     /locus_tag="VN039_14695"
     CDS             complement(5539..6063)
                     /locus_tag="VN039_14695"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HWV47245.1"
                     /translation="MSKPKRTDADRLIRRYYEGENTGQQGAFPEDTLASVPHVDPRSS
                     DGMTPDVTLSGGDVDATRSGTDDGEEAVGGSTPTPDQDVVDELGEAAGITYHEAEPLR
                     FGDKVADRDAQRWELNPASSEGYQERLRELTGSGRPTPTSPRTKQPKQAARTKQSTPG
                     RHTVGARKEQKKNR"
     gene            complement(6132..6584)
                     /locus_tag="VN039_14700"
     CDS             complement(6132..6584)
                     /locus_tag="VN039_14700"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HWV47246.1"
                     /translation="MAPTGKHLSFLLIFLVGAAGCDATRELVKAPFEATTALSNGTTQ
                     ATNELLEPLKEFTSSTTPGSMYNDPLKAKRRFQVFIAYTFNNVQRDIAQGHGEYLTSL
                     ATLAGIPEERRPQFFAMLQDRYGTIYSADTALSESLQRLTAAAWPERN"
     gene            complement(6689..7852)
                     /locus_tag="VN039_14705"
     CDS             complement(6689..7852)
                     /locus_tag="VN039_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWV47247.1"
                     /translation="MSSFRPVPTPTSSPSAETMHDKPGVDRHLWEIRPIRDLFIFGAI
                     AGLLWSLYELHTVFLPVFIAIFFAYLVNPFVDYASTHWSVPRPLTVGILMLFLTLIFV
                     GLGLWLIPLLIEQAQTLIQKIPVYVRSLSQRYQDLLSNSFNLRDILGRMYDDPLATLQ
                     PLFVGTGHAFGLLGQVLGITVNAALYTILIPIYFFFFAWNFERMSQTVVRLIPIVHRP
                     HILHVLSRMDTAVRGFVSERLLIAVITAVLYAIGWELTEVPYWFLLGVAIGLASLVPY
                     LSAIGWPLAVILKYADAVTAGQSTETEWMAILLWPSLAYLIVQFIESWVLTPWLQSHS
                     TDMGAATILIVVFLGGAVGGLFGLIFAIPAATCIKILIEEYIRPRWLRWVREA"
     gene            complement(8027..8404)
                     /locus_tag="VN039_14710"
     CDS             complement(8027..8404)
                     /locus_tag="VN039_14710"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HWV47248.1"
                     /translation="MESIGRRRWAIAEGYIPMGSHGPSPQMTSHETVCLLNTSDRDAL
                     MRLTVFYSDREPVGPYQLVVPGRRTRHVRFNDLNDPEPIRRDTDFAGLGESDVPIVVQ
                     HTRLDSRQAENALLSTIAFAGDG"
BASE COUNT         1970 a         2345 c         2509 g         1922 t
ORIGIN      
        1 cacaagatgc gatccatctg aaaaggtcag aagcggatct aagttgatgc gactcatcgg
       61 cgcgctcctt cggctagtcg ggcagacttt acgtatgcgg atgcataggg catgacgaaa
      121 ctcgcaaaca attgccctca aacggcacac tttcagtgcg acgaccgtac acggcgcttg
      181 cttgcggacc ccatttccac acctgtgcga atcagttcag cctgagatgg cgttacttct
      241 ggacaaggcc gttcagagaa ctctggcctc ggagcggcgg agactgagga atcgatcacg
      301 ctgagaagaa gtaggaagcg taagagatgg gacagtcacc gaagcttcac agtatcacct
      361 tgggcgcaat gggcatgaga gtcaagcact attgtggatc gggacaacag gcacgttcga
      421 aagctggact cacggtgcgg caaagtgcct tgatcgcaca tcgtgaaata gaaatgaatt
      481 ggttcaaggc gagagacccc acaatggcta aactcattct atcgaacgtg cgccacgcca
      541 gaatccactt ttagccacag aaaagccaca atctcaagag acaatcacca atcagaaact
      601 ccttcgggca atgcaacacg ttggatttgg tgccgaagac catcccatgc agccgtatgt
      661 cctattgagt ttgttcgctc cggctggtgt tgatcgggtg gttgtccttg atgccgccgg
      721 ctccggccat gctcaagtga aaatcatcgg cggtctgcgc ggagggccgc tggagacgat
      781 tcctcagttg gttcgcgcga tgacgtatgc gcgtgatcag gccttgttca gccggaacct
      841 gatttgaaag ttgctccagc gcatgctccc acagggtcaa ggccccggtc gactcgccgt
      901 ggcgttcgag ctcaatgccg agtcggtagg ctgccaatga ggaagcagac ggacaatggg
      961 cgacggcttg aaacgcccgc ctggcctctt gagaatcacc ctgttcagcc atcgtgttcc
     1021 cgaggcccag cagggctcgc gaatagcagg agtctcgctg caaggcctgt cggtagaagg
     1081 tttccgcctt gtcgaattgg cgctgctgcc ggcgaagatc gccggcgtcg gtcaagagtt
     1141 cgagatcatc gggcaggcgt gccagagccg cttcatatct cgcccatgca tcattgacgt
     1201 tgcccttgga agccaatgcc cggccgagat catgcaaaat atccggacga tcggattcca
     1261 gcaacaatgc cgattcataa tgttggatgg ccatatcgag gaggtcggga tctctccgaa
     1321 tgacgtcggc catcaactgt agtaaatgag ccgtggtgac gaatccgtcg gacgcggtcg
     1381 gcttcggcat ggccggttgg gtttgaccgg gtggttcatt aactccgccc gataagagat
     1441 gatcggcgtc gagccattcg cgaattctcg atgagggaat gcaataactc aagcccgctc
     1501 gtaacatgac ccgtccgatc gactcgccct gcgtattttt agccgtctcc agatacggaa
     1561 cggtatggac gacgagtccg agaatatgcc cggacgcggc cgcgacgagc ggaccgccgc
     1621 tgcttcccgc attcatgagc ccggtaatgt gaagaaaggg gagcggtctg tgggcgatgc
     1681 gcgactgtcc catcgcttct ccgctcactg tgacagggaa tactgtcgga tggatcgtca
     1741 atcccatctg aagcacatcc ggcaagcgag gatatccgat cgtcacggcg gccatgccgt
     1801 cttctgccgg cttgctcgcc aacgacaaat acgggacggg atgagcgtat gggatgcgta
     1861 gcgccgcaag gtcgagatca ggatccaccg cgacgatctc gggagtcggt gtaaggctat
     1921 cggccgcccc atagaccaca atacgagtgg cttcatggat gacatgcgcg ttggtaagca
     1981 gcaagccgtc ttgagagatg aacacgccgc tgccgaagga aagggcatgg gcttcatcat
     2041 cgataccgat gacgagcgct gtcgcttcct tgccggaacg gtagagatcg cctgccgtca
     2101 acccccaggc gggccatgca gtgaacagtc ccgcgcagac taggatggta cggacggcaa
     2161 ggagactatg gagccctgcg agtgtgatgg tgtccctcgg tgcgacggga tgcactgtac
     2221 actacgcagg gatggtgaag aaagccagta gaatctcata aacagaattg cagcgaaact
     2281 aggcgaaccc ccggtctatg aggctactgt cccgcctcat actcacatct actcgcaaat
     2341 cttcgaacca tgacgctgag gacgagcgcg cggcgcaagg tctacgcgtc gtcccgacga
     2401 tcttcttgtc gcgcaatttt ctccagcact tcggcctgat gttggcacga caagcaacgt
     2461 tttgcgaagg ggagggccct caggcgtcct gcgcctatct tggcaccgca atcctcgcac
     2521 aatccgtact ctccatcatc cagtcggcgg agtgcagtat cgagcatcat gcgagtgcgg
     2581 ttacgcgctt ccagcaacga taattgttgt tcggccgtgc tgtcgcgtaa ggacatatct
     2641 tcaaggtcga ggacggagtg attgtgggtt gcagtgtagt gctcccgata ctgggtcaaa
     2701 agttcatgga tctcttgttg cacagcagag cgtctctcaa gcagcattgc tcgaaggtcg
     2761 tccgccgatg tggaagtagg ttcaatgagt gtcttggcca tgatcaagct ccttcctatt
     2821 gaagtgtgga tcagtgcagc acctttgctt gatctgcaaa aatgaggccg ccgcttgtat
     2881 cgttcgcatg cttcccaaaa tgaaaaattc caggatcaat tccgacaatg catcgctttt
     2941 ttcaagagcg gaccgatgag gtatttcttt gccaatgcaa tgtccataca ggatgaagga
     3001 caagttgtcc tttcgagaag catattctaa tactcgttac tcgttaagag tggggggcga
     3061 tacgacctct tgacccgtga gatcttggat accatgggta ttacgattgc acctctaccg
     3121 aatcactcgg caattttcta gggttggtat cgtaaccatg cgtctcacgg tggccacgta
     3181 taatgtacac cgctgcattg gatgggatgg gcggtatggc cgggaacgta ttcttgcagt
     3241 gctgcaggaa ttgcaagccg atctcattgc gctacaggaa gtggatgagg aaaacacctt
     3301 gcagtggttg gccgttcact tgagcatgac cgcgatcgcc ggaccgaatt tacggcgcca
     3361 cggcgcctac ggcaacggcc tgctgacgac gtgcaagttg ggagaattcc ggttagtcga
     3421 tctcagtttc cgtggccatg aaccccgcgg agccatcgat gtcgatgtgg tgtgcggcaa
     3481 tgagagggtt caggtcatcg cgacccatct gggtttacgg ccgagcgaaa gacgctggca
     3541 agtgcgccaa ctcctcacac ggttcggcac gcgccattgc attcttttgg gggacatcaa
     3601 cgaatggttt ttatggggac gacctcttcg gtggatcacc aagatgtttg gacgcacgcc
     3661 ctctctccga tcatttccct ctccctggcc tattttcccc ctcgaccgta tctggatcag
     3721 gcctccgggc agcgtcatcg aaattcgaac tcataagaca gccctctcaa gcgtggcttc
     3781 agatcatttg ccggtaaagg ccatcattga attgtaaact cacggaatcg tgcgctccag
     3841 acgatgttca acggccttcc agttgatatt ggaaaaaaag gcctcgatgt acgagacgcg
     3901 ttccgaaggt ttgtaatcca ggataaaggc atgttcccac acatccatga ccagcacagg
     3961 ggcgaagccg gccacattgc cggtttcatg cagcgtgatc caatgattgg tgagtttgcc
     4021 gttgtaggga ttgagataac atacggccca gccgactccc cgcattttcc ccacgccgat
     4081 gaaatcgcct ttccaggctt catacgtgcc gaagctgctt tcggccgcgc gtctgaacga
     4141 ggagtgcgcc cccggatcgg gcgatccgtt ggccatgagg ttatcgaaat agtattcgtg
     4201 caggaccatg ccgttgtatt cgaacccgta tcgccgtttg agctccgagt agaccggcat
     4261 ctcttcctga tcgacttttc cgtcctccac cagttgatga attcgcgccg tgagattatt
     4321 cgtctccttg acgtaacctt cgtagagttt gaaatgcatg gcgagcgtcc ggtctgaaat
     4381 gccgtgaagg tcgtggagat cataggatcg tggttcatac tttcgctgca gtgttgcggt
     4441 gggcattgtg tcgctctcct tcatgtgaat ggttcatgtt cagacaatcg catattcata
     4501 caatgtgaag cgactcacgt ctgagaagct gaggactcat gcacgagtcg cagacgaaac
     4561 cccgggtacg aaatcgtaca gaggcacttc acgagccatg gcatgatgcc gaaggctccg
     4621 atcaagcttg ccagcgcgaa tggatagaaa gccgctcccc aggaagggct cgtgcgggag
     4681 agttcgggca tgccgatgat gatcagtccg ttgacaagca tcagcaacag caccacgctc
     4741 cccacgcccc gccagagaat agtccatccg atccggagcg cttcccaata tgtcggtacc
     4801 atgtgagaca tccttttcaa ccttaggttg aatcaaggcc cggcgagggc gacggatgca
     4861 ccggattggg cttgggagga ggaggctgag gaggcggaat cggcccggac ggtttgggag
     4921 tcgggtcggt cggtcctggg ctcgggtccg tcggcgggct ggcttcatgc ggacggacga
     4981 gaattgcagc ctgcatcgac gcggatacta aataggcaac gagcaacagg ttgatcgcaa
     5041 aagccttcaa catgcacctc ttcttcagaa agccgccgct tgtccggctc ttatgcaagt
     5101 catgccgagg ggacgacggt cccgaccatt tcacgacaga gatccgcgca ccgacgacat
     5161 tccttcgcgc atggaccgca caaggcatgt tgcggcgcat ggcgctcgca cacttcagca
     5221 cactcatcgc aaatctccgc gcagagccgg catagagact cggcctgctg tgaggagcgg
     5281 ctgatgaatt ttgcggcgag gacgcatatg tcggcgcaat cgcggcacag tctgatacaa
     5341 cgtgtcatca gatcattatg gtcgtgatgt tccatgccga tcatatcgtt cgcgcaggcc
     5401 tcgcaggttt gcgcgcatga gaaacaggcg ttcaggcaat tctgatgctg ttgcgagaga
     5461 gtcttcatct ccataatagt cggctccttt cctgtccata catcgactca ttaaaaaaca
     5521 tcgactcatc aaaacatgtc agcgattttt cttttgctcc tttctggcgc cgaccgtatg
     5581 gcggccgggt gtgctttgtt tggtcctggc ggcttgcttc ggttgtttcg tccggggact
     5641 tgtcggtgtc ggtctgccgg agccggtgag ttcccgcaat cgttcctgat atccttcgga
     5701 agaggcggga ttcaattccc atcgctgcgc atcgcgatcg gccactttat cgccgaaccg
     5761 caagggctcg gcttcatgat aggtgatgcc tgccgcctcc cctaattcgt caaccacgtc
     5821 ctgatccggg gttggagtcg aaccgcccac agcctcttca ccgtcgtccg tcccgctccg
     5881 cgtcgcatcg acatcgccgc cggacagtgt tacgtcgggc gtcatcccat cggaggagcg
     5941 cggatcgaca tgcggaacag aggccagcgt atcttccggg aaagcccctt gttgtcccgt
     6001 gttctctccc tcatagtacc ggcggatgag gcgatcggcg tccgtgcgtt tcggttttga
     6061 catggtccat ccttcaatgt gtggttcgtt cataagatta tgtgaacggc gaccgggttg
     6121 aaattgcgtt ctcagttcct ctcaggccaa gccgcggctg ttaaccgctg caaggattcg
     6181 ctcagcgcag tatctgcgga atagatggtg ccatagcgat cttgcagcat ggcaaaaaat
     6241 tgggggcgtc gttcttccgg gatgcccgca agggttgcga gcgacgtcag atattccccg
     6301 tggccttggg cgatgtctct ctggacgtta ttgaacgtat aggcaataaa tacttggaat
     6361 cgacgcttcg ccttgagtgg atcgttatac atcgaaccgg gagtggtgct ggaagtaaac
     6421 tccttgagcg gctcgaggag ttcatttgta gcttgcgtcg tcccattgga cagcgctgtc
     6481 gtcgcttcaa aaggtgcttt gaccaattct ctcgttgcat cgcatcccgc agctccaacc
     6541 agaaatatca gcaaaaacga aagatgcttt cctgttggtg ccatggcaag ctcctttctt
     6601 ccgataccgc ccgatatcct gcccctgttt ccaatgtacg caggctcctc agacggctgt
     6661 actcggtcat tccctagatt tgatgggtct aagcctcgcg tacccatcga agccatcgcg
     6721 gcctaatata ttcctcgatc agaattttga tacaggtggc ggcgggaatc gcaaagatca
     6781 gcccgaacag ccctcctacg gctccgccca agaagaccac gatgagaatc gtcgcggcgc
     6841 ccatgtctgt ggagtggctt tgcagccagg gagtcaagac ccagctttcg atgaattgca
     6901 cgatcaggta ggccaggctc ggccagagca aaatggccat ccattctgtc tctgtggatt
     6961 gaccggcggt cacagcatcg gcgtacttca gtatgacggc taagggccat ccaatggcgg
     7021 agaggtacgg caccagtgag gctaaaccga tagccacgcc caacaggaac caatagggga
     7081 cttcggtcaa ctcccatcct attgcgtaca atacagcggt gatcaccgca ataagaagac
     7141 gttcggaaac gaagccacgc acggccgtat ccattcggct caagacatgc agaatgtgcg
     7201 gtcgatgcac gataggaatc aggcggacga cggtctgact catgcgttca aaattccaag
     7261 cgaagaagaa aaagtagatg gggatcaaga tcgtatagag cgcggcattg actgtgatcc
     7321 cgagcacctg tcccagcagg ccgaatgcgt gaccggtccc aacgaaaaga ggttggaggg
     7381 ttgccagcgg gtcgtcgtac atccgcccaa ggatgtcgcg taaattgaag gagttactca
     7441 gtaggtcctg atagcgttgt gaaagactcc gtacatagac cggaattttc tgtatcaatg
     7501 tctgagcttg ttcgatcaag agggggatga gccacaatcc gaggccgacg aagatgagag
     7561 tcaagaacag catgagtatg ccgaccgtca aggggcgagg caccgaccaa tgtgtggacg
     7621 catagtcgac aaacggatta accagatagg cgaagaaaat ggcgataaac acaggcagga
     7681 agacagtatg caattcgtag agagaccaca gcagcccggc gatggcgccg aagatgaaca
     7741 ggtcgcgaat ggggcggatc tcccataagt gtcggtcgac tcctggcttg tcatgcatgg
     7801 tttcggccga tggtgaggac gtcggtgtcg gcacaggacg aaacgaactc atcgttgaat
     7861 cactgcatat ccaagcgtga tagtgatgcc ggatggtttc cccttgtgcc ctcgggaacg
     7921 gccgacgcca gagccatcgg aatgaccgga ctgacgagaa gaaggaagga gagtgcctgc
     7981 cgtcgtaaac agtgtcgtga ggttggttgt ttcacgaatc acataatcac ccgtcacccg
     8041 caaatgcgat ggtgctgagc aaggcgtttt ccgcctggcg agaatcaagg cgggtgtgct
     8101 gtacgacgat gggcacatcc gattcgccga gaccggcaaa atcggtatcg cgccggatcg
     8161 gctccggatc attgagatcg ttgaagcgga catgcctcgt tcgtcgtccc ggcactacga
     8221 gctggtacgg cccgaccggt tcacgatcgc tataaaagac ggtgaggcgc atcagggcgt
     8281 cccgatcgga tgtattcaac agacacactg tttcatggct ggtcatctgc ggcgaggggc
     8341 catggctgcc catgggaata tagccctctg cgatggccca tcgtctgcgt ccgatacttt
     8401 ccatattggc aaccgccagg ctaaaaacac tgcaagcgag gtgcctagta aggttagcag
     8461 tggtcattta cggcagggct tcacccgcct acgcaggaag ccacggttga ctcttctcag
     8521 cagtggtctg ctgcggagga tggatcaagc agtggggctc caccgggctt tcttggaaga
     8581 cttgtctcag aatagacatc ttgtccgaag tatgtcatcc atgctgttct tctcagaggg
     8641 tggttggcga agtattcaga agcattcagc ggtagattga agggatgcac ctttaggcgg
     8701 aagggcagga aggaaaatga atggccacaa gcggcctatg ttttgc
//
LOCUS       DATMTI010000934         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_162203, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000934 DATMTI010000000
VERSION     DATMTI010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162203"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VN039_14715"
     CDS             <1..1352
                     /locus_tag="VN039_14715"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV47249.1"
                     /translation="AQRRIENILVEIGVLERESATGTNHDERLQELRQEQELVQQRLE
                     RLKKQWDEEARIIGRIRDVRGQLEELALGSTDKANTSEAGADPASLKQELERLNAELE
                     TLQGETPLMQACVNSQTVAEVISGWTGVPVGKMLSDEIQTVMALEERLKQRIMGQDHA
                     LTAISRRIRTSRASLTDPRKPIGVFLFVGPSGVGKTETALTLADTLYGGERNLITINM
                     SEYQEAHTVSSLKGSPPGYVGYGEGGVLTEAVRRRPYSVVLLDEVEKAHPDVMELFFQ
                     VFDKGLLEDGEGREIDFKNTIILLTTNVATDTIMKLCADPDTRPVPDALTEAVRPELM
                     RVFKPALLGRMAVVTFYPLTDGMMKDIITLSLSRVRSRVEQNHRAQFTYDDAVVAEIL
                     NRCKEVESGGRNIEHIISKTLLPDMSGEFLSRMMTGRPITRVHVGVGGQGGFSYDIV"
     gene            1388..3511
                     /gene="tssI"
                     /locus_tag="VN039_14720"
     CDS             1388..3511
                     /gene="tssI"
                     /locus_tag="VN039_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HWV47250.1"
                     /translation="MADGQTYTQKGRPIAVKTPLPDDTLLLVGISGREGLSQLFSFHL
                     DMLTEDRKAKDVSFDKLIGKSITVTLTRSDKAKSKRFFNGICSRISETGKAGEFTAYQ
                     MEIVPKLWLSSRKSHSRIFQNETVPNILKDVLSGIQPKFEIHGKFEPRNYCVQYRETD
                     FNFVSRLMEEEGIYYFFTHAHGQHTMVVADTPQHPALTPIRYDEVSGGTREDEHIFSW
                     QKVQELRTGKYTLWDHHFQAPDKTLEANESGVSSVQVGKVSHQLVVEANRDLELYDFP
                     GEYAQRFDGINRGGGEQTAEVNKIFEDNKRTAKIRMQQEEVSSLVIHGAGNCGHLLGG
                     HKFKLDRHPTAEGEYVVTSVNHTAQAGDFRSDSTDEFTYQNSFSCIPVALPFRPLRNT
                     PKPMIPGAQTAVVVGPSGEEIFTDKYGRVKVQFFWDRDGKKNEQSSCWVRVATTWAGG
                     KWGGIHIPRIGQEVVVAFVEADPDAPIIVGSVYNSDQMPPYELPANKTQSGIKTRSTL
                     NGSHDNFNEIRFEDKKGQEQLFVHAERNQDIEVEKDETHWVGHDRKKKIDNDETTTIG
                     RDRKEDVGKNETISVGENRSESVGKNEDISIGKNRTENVSENESVTIGKNRTEQVGKN
                     EEVSVGENRSHTVGKNDQLDIGKQLLVTAGDSITFNTGSASLILKKNGDIQLRGNNIL
                     VDGSGKVNVKASGDVVLKGAKVTQN"
BASE COUNT          944 a          902 c         1103 g          656 t
ORIGIN      
        1 atgcacaacg acgcatcgag aatatcctgg tcgaaatcgg cgtgctggag cgagagtccg
       61 cgaccggaac gaaccatgac gagcgactgc aggagttaag acaagaacag gaattggtac
      121 agcaacgact cgagcggctc aagaagcagt gggacgaaga ggcaagaatc atcggacgga
      181 tacgagacgt gcgtgggcaa ctggaagagc tggcactggg ctcgaccgat aaggccaaca
      241 cgtcggaagc cggcgcagac ccggcgtcgt tgaagcagga attggaacgc ctgaatgccg
      301 agctcgaaac gcttcaggga gaaacaccat tgatgcaggc ctgtgtgaat tcacagacgg
      361 tcgcggaggt catctccggt tggaccggcg tgccggtagg gaagatgtta tccgatgaga
      421 tccagaccgt catggcgctg gaggagcggc tcaaacaacg gatcatgggg caggatcacg
      481 cactgacggc catcagccgg aggattcgca cctcaagggc cagtctcacg gatccgcgga
      541 agccgatcgg cgtgttcctg tttgtcgggc cgagcggagt ggggaagaca gaaaccgcgc
      601 tcacgttggc cgacacgttg tatggaggcg agcggaacct cattaccatc aatatgagcg
      661 aatatcagga ggcccatacg gtgtcctcct tgaaaggctc gcccccgggc tacgtcggct
      721 atggggaagg aggcgtgctc acggaagccg tgcggcggag gccctattcc gtggtcctgc
      781 tcgacgaggt ggagaaagcc catcccgatg tcatggagtt gttcttccag gtgttcgaca
      841 aggggctgct cgaagatgga gaaggccggg agatcgactt caaaaacacc atcattctgc
      901 tgaccacaaa tgtcgcgacg gacacgatca tgaaattgtg cgccgatccg gatacgaggc
      961 cggtgcctga tgccttgacc gaagccgttc gaccggaact catgagggta ttcaagccgg
     1021 cgctgctcgg acggatggcg gtggtgacct tttatccgct gacggacggc atgatgaagg
     1081 acatcatcac gctcagcctg tcccgtgtcc ggtcgcgtgt cgagcaaaac cacagggcac
     1141 agtttacgta cgacgatgcg gtcgttgccg agattttgaa ccgctgcaag gaggtcgaaa
     1201 gcggcggacg gaacatcgaa cacatcattt ccaagacgct gctgcccgac atgtcgggcg
     1261 agttcctgtc acggatgatg acgggccgac cgattaccag ggtgcatgta ggagtcgggg
     1321 ggcagggcgg attcagttac gacatcgtgt gagaacgaga atatgaacag aaagaggctc
     1381 ggtttccgtg gctgacgggc agacatacac gcaaaaaggc cggccgatcg ccgtcaagac
     1441 tcccttgccg gacgacacgc tgctgttggt cggcatcagc ggccgagaag ggctctcaca
     1501 acttttttcg tttcacctcg acatgctcac ggaggacagg aaggcaaaag acgtctcctt
     1561 cgacaaactc atcggcaaga gcattaccgt gaccttaacg cgttccgaca aggccaagag
     1621 caagcgcttc tttaacggga tctgttcacg aatcagcgag acagggaaag caggcgagtt
     1681 taccgcgtat caaatggaga tcgtgccgaa gctctggttg tcctcccgca agtcgcacag
     1741 tcggattttc cagaatgaaa ccgtcccaaa tattttgaaa gacgtcctca gcggcataca
     1801 acccaagttt gaaatccatg ggaagttcga gccgaggaac tattgcgtgc aatatcgtga
     1861 aacggacttt aactttgtca gtcgcctcat ggaggaggaa ggcatttact acttctttac
     1921 ccatgcccat ggccagcaca cgatggtggt ggccgatact ccgcagcatc ccgctctgac
     1981 gcctatcagg tacgacgagg tgagcggcgg caccagggaa gatgagcata ttttcagttg
     2041 gcaaaaagta caggaactgc gtaccggcaa gtacacgtta tgggaccatc attttcaggc
     2101 gccggataag acgctggaag caaacgaaag cggagtttct tctgtgcagg tcggcaaagt
     2161 gagtcatcag ttggttgtcg aggccaacag agacctggag ctgtatgact ttccgggtga
     2221 atatgcccaa cgttttgacg gaatcaaccg cgggggcggt gagcagacgg cggaggtcaa
     2281 taagattttc gaggataata aacgcacagc caagatccgt atgcagcagg aggaagtgtc
     2341 gagcctcgtc atccatggag ccggcaattg cgggcatctg ctcggcgggc ataagttcaa
     2401 gctggatcgg catccgacgg cggaaggcga gtatgttgtg accagcgtga atcacactgc
     2461 gcaggcagga gatttccggt cggattcgac cgacgagttt acctaccaga attcgttcag
     2521 ctgcattccg gtcgctttgc cctttcgccc gttacgcaat accccaaaac cgatgatccc
     2581 cggcgcgcag accgccgtgg tggtcggacc gtccggagaa gagatcttca ccgacaagta
     2641 tggacgggtc aaagtccagt ttttttggga ccgtgacggc aagaagaacg aacagagttc
     2701 ctgttgggtg cgggtcgcca ccacctgggc cggcggcaaa tggggcggta tccatattcc
     2761 ccgcatcgga caggaagtcg tggtggcatt cgtggaggca gatccggacg cgccgatcat
     2821 cgtcggcagc gtctataaca gcgaccagat gcctccctac gagctgccgg ccaacaagac
     2881 ccagagcggc atcaagactc gatcgacgtt gaacggcagc catgacaact tcaatgagat
     2941 tcgctttgag gataagaagg gtcaggagca gctctttgtc cacgcggaac gcaatcagga
     3001 tatcgaggtg gaaaaggacg agacccactg ggtcggccat gatcggaaga aaaaaatcga
     3061 caacgacgag accacgacga tcggccgcga tcgaaaagag gacgtgggca agaacgaaac
     3121 catttcggtc ggggaaaacc ggagcgaatc cgtcggaaaa aatgaagaca tcagcatcgg
     3181 caagaacagg accgagaacg tcagcgagaa tgagagcgtg acgatcggga agaatcgaac
     3241 cgaacaggtg ggcaagaacg aagaagtctc agttggagaa aaccggtcgc atacggtggg
     3301 caagaatgac cagttggaca tcgggaaaca gctcctcgtg acggcaggcg atagcatcac
     3361 cttcaacacg ggaagcgcca gtctgattct caaaaagaac ggcgacattc aactgagggg
     3421 caacaatatc ttggtcgatg gatctggaaa ggtgaatgtc aaagcatctg gtgatgtggt
     3481 gttgaagggg gcaaaagtaa ctcaaaacta agaagctaag cgagttccca gtcatgaaat
     3541 acgaaaaaat taacgtaagt aacaacggtg ttaacggcag tgttgtcagt gaacaggcat
     3601 ggctt
//
LOCUS       DATMTI010000935         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_544587, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000935 DATMTI010000000
VERSION     DATMTI010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544587"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VN039_14725"
     CDS             complement(<1..346)
                     /locus_tag="VN039_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWV47251.1"
                     /translation="MRFAIRLALTNVTAKTGGPFGAAVFETKTGQLIAVGVNIVEPSN
                     CSLAHAEMVALANAHHALRHFDLGADGMPNFDLVTSCEPCAMCYGAIMWSGVQKVMCG
                     ARGADALAIGFDE"
     gene            complement(445..762)
                     /locus_tag="VN039_14730"
     CDS             complement(445..762)
                     /locus_tag="VN039_14730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV47252.1"
                     /translation="MPNPRIEPLKKVLAIDPSDDVAWFGLGKAYMDDGNFEEAAKSLR
                     QCVTVKPTYSAAYLALAQSLQKLGRLDECRTVCATGIDISTKNGDAMVTKNLEALKLS
                     LPS"
     gene            889..1872
                     /locus_tag="VN039_14735"
     CDS             889..1872
                     /locus_tag="VN039_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWV47253.1"
                     /translation="MRSKTDVDVPGYLMGSTGLVYHPAYLEHDMGPGHPESSNRLRAI
                     IQRLEQSGTMARMIKIEARKAEDEWLTQIHSPNYVLSLNRHAPTSGRVSLDADTSMSP
                     GSLNAAYLAAGGLLAAVDAIMARQVDHVFCAVRPPGHHAEAERAMGFCLLNNVAIAAR
                     YVQKKYGVTRVLIVDWDVHHGNGTQHSFEDDPSILFFSTHQYPHYPGTGRGTERGKGA
                     GVGFTVNVPMEEGEGDEAYHAIFLKSLVPAADAFKPEFVIISAGFDAHKDDPLAGMGL
                     TEAGYAALTEIVAGIAKRHAKGRILSSLEGGYNLTALAHSVDAHINALFNA"
     gene            1909..2787
                     /locus_tag="VN039_14740"
     CDS             1909..2787
                     /locus_tag="VN039_14740"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWV47254.1"
                     /translation="MKHPLLIDTETLQQHLGRPDLVVIDVRGKSAYEFGGHIPGAVHS
                     TWHEYSDPNAVPKGLLNPDLGQIERILSRLGMNQGSDVVIYSNPFDNWGDEGRMFWML
                     EYLGHKRLRILDGGWVKWTAEKRPFEHGPVSPMMGNFKAQPVQALIALKDDLKGLIRG
                     PHPQTAILDARSVEEYLGKEVSGLPRSGHIPSAIHVAWNGFLNKDATVKDLSVIKEML
                     EVKGVRNGQEVICYCTGGVRSAWLYFILKLVEYPKISNYPGSWWEWSRDFACPAEKDV
                     HALQKILEFDPAAKPS"
     gene            2904..3215
                     /gene="smbP"
                     /locus_tag="VN039_14745"
     CDS             2904..3215
                     /gene="smbP"
                     /locus_tag="VN039_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HWV47255.1"
                     /translation="MAAIGALVGMPMLSGVAFAADKHAAEAVEHAKAAVSHGKEGHAD
                     ACVEHASEALKHAQATMKNPHGAEGVKHLTEAVKHGKAGHADACTEHAEGAATHLAEV
                     K"
     gene            3309..>3548
                     /locus_tag="VN039_14750"
     CDS             3309..>3548
                     /locus_tag="VN039_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWV47256.1"
                     /translation="MQVGFYQFNPQFGEVAKNLDVVAAKLEQVEADLIVLPELFASGY
                     QFLSREEVQQFAEPVPDGQTVRCLIDIAKRRKMHLV"
BASE COUNT          778 a          905 c         1071 g          794 t
ORIGIN      
        1 cttcatcgaa cccgatcgcc aatgcatcgg cccctcgtgc gccgcacatg actttttgga
       61 caccggacca catgatcgcc ccgtaacaca tggcacaggg ttcacaactc gtcaccaggt
      121 cgaaattcgg catgccgtcc gccccgaggt cgaaatgtct cagggcgtgg tgtgcattcg
      181 cgagcgctac catttcggcg tgggcgagcg agcagttgct gggctcgacg atgtttaccc
      241 cgacggcgat gagttgccct gtcttggttt cgaacactgc tgcgccgaaa ggtccgccag
      301 tcttggctgt gacgttggtc aacgcgagtc ggatggcgaa tcgcatgcga tcaccagccg
      361 ctaaaatggg cgtttgctgg cggcgcgcag cgcggatggt ccaagatgga aggcgaaagg
      421 gagaagctgg cgagcgtccg gttttcacga tggcagtgaa agtttcaagg cttcgaggtt
      481 ctttgtgacc atggcatcgc cgttttttgt tgatatgtcg atgcctgtgg cacagacggt
      541 tcgacactca tccagacgtc ccagtttttg gagtgactga gcaagtgcga gataggcggc
      601 tgaataggtt ggtttcacgg tcacgcactg gcgtaaggat ttagcggctt cttcgaagtt
      661 cccatcgtcc atataggctt tccctagtcc gaaccaggcg acgtcgtcgc tcggatcaat
      721 ggcaaggact ttcttcagtg gttcgattct tgggttcggc atgtgtactc ctgtcgttga
      781 tggcagacga tagcagcgct gaagcacatt gtctatggag ttttctgcat gataatctga
      841 accggatccg gaagttctcg gtatattcgt gctccgtacg atcgtcgcgt gaggagcaaa
      901 acggatgtcg acgttcctgg ttatctaatg ggaagcactg gtctcgtcta tcatcccgcc
      961 tatcttgagc atgacatggg accagggcat ccggagtcct ccaatcggct gcgcgcgatc
     1021 atacagcggc ttgaacagag cggtacgatg gcccgcatga tcaagatcga agcgcgcaag
     1081 gcggaagatg aatggcttac gcagattcat tcgccgaact atgtgctgtc gctgaaccgg
     1141 cacgcgccga cgagcggccg tgtctcgctc gatgccgata cctcgatgtc gcccggctca
     1201 ttgaatgccg catacttggc ggcaggaggc ctattggcgg cggtcgatgc catcatggca
     1261 cggcaagtgg atcatgtgtt ttgtgccgtc cggcctcctg gtcaccatgc cgaagcagag
     1321 cgcgcgatgg gtttctgcct cttgaataac gtcgccatcg ccgcccgcta tgtccaaaag
     1381 aaatacgggg tcaccagagt gctgatcgtc gattgggatg ttcatcatgg gaatgggaca
     1441 cagcacagtt tcgaggacga tccctctata ctttttttta gcacacatca gtatccgcac
     1501 taccccggta cgggccgagg caccgaacga ggcaagggag cgggagtagg atttacagtc
     1561 aatgtcccga tggaagaagg ggaaggcgat gaggcgtacc acgcgatctt tctcaaatcg
     1621 cttgtcccag cggccgacgc gttcaagccg gagttcgtca ttatttcggc gggattcgat
     1681 gcgcacaaag atgacccgtt ggccggcatg ggtttgacag aggcaggata tgccgctctg
     1741 acggaaatcg tggctggtat cgccaagcgc catgcgaaag gccgcatcct gtcttctctc
     1801 gaaggtgggt acaatctgac ggcgttggct cactcagtgg atgcgcacat caacgcgtta
     1861 ttcaatgctt gaatcacgct accgcgaact gtaacgaaag aagatatcat gaagcatccg
     1921 ttattaattg ataccgaaac gcttcagcag cacttaggcc gtcctgatct tgtcgtcatc
     1981 gatgtccggg gcaagtcggc gtacgaattc ggcggacata tccccggagc cgtgcattcg
     2041 acttggcatg agtacagtga tcccaatgcc gtgcccaagg gattgctcaa tcccgacctc
     2101 ggtcagatag aacgtatctt gagtcggctc ggcatgaatc aaggcagcga cgtcgtgatc
     2161 tactccaatc cattcgacaa ttggggtgat gagggacgga tgttttggat gctggaatat
     2221 ctgggccaca agcgcctgcg tattttggat ggtgggtggg tgaaatggac ggccgagaag
     2281 cggccattcg aacatgggcc ggtctctccg atgatgggaa atttcaaggc ccaaccggtg
     2341 caagctctga tcgcgttgaa ggacgatctg aaaggcctca tcagggggcc gcatccccag
     2401 acggccattc tggatgcgcg cagtgttgaa gaatatcttg ggaaagaggt gtccggtctt
     2461 ccacggtccg gtcacatccc ctccgccatc cacgtggcgt ggaatggctt cttgaacaag
     2521 gatgcgaccg tcaaggatct ctccgtgatc aaggagatgt tggaggtaaa gggggtacgg
     2581 aacgggcagg aagttatttg ctactgtacc ggcggtgtgc gatcggcttg gctctatttc
     2641 attctcaagc tggtcgagta cccgaagatc agcaattatc ccggatcatg gtgggaatgg
     2701 agtcgggatt tcgcctgccc agccgaaaag gatgttcatg cgctccagaa aattctcgag
     2761 tttgacccag ccgccaaacc ttcttgacag cctgagtagc gctgtgtaaa ggtgaattca
     2821 cggtcgattg ttctcttgag cagcgaattt caaccacgga ggaggcaacg atgacgacca
     2881 cgatgcgtcg aagtgctttg ataatggctg caattggagc gctcgtcggc atgccgatgc
     2941 tgagcggagt ggcatttgcc gccgacaagc atgcggctga agccgttgaa cacgcgaaag
     3001 ccgctgtgtc gcatggaaaa gagggccatg ccgatgcttg tgttgagcat gcgagtgaag
     3061 cactcaagca tgctcaggcg acaatgaaga acccacatgg ggcggaaggt gtgaaacact
     3121 taacggaagc cgtgaaacat ggtaaagccg ggcatgccga tgcctgtacc gaacatgccg
     3181 aaggggcggc cacgcatctg gctgaggtga agtagttgac gagaagcagc cgcggctgct
     3241 gcaaggtgcc tttgttgaag cgggcaggga cacaatccct gcccgctttt atttgaacgg
     3301 agtgtgccgt gcaagtcggg ttttaccagt tcaatccaca gttcggcgaa gtggcgaaga
     3361 atctcgatgt cgtcgcggcc aagttggaac aggtagaagc ggatctcatc gtgctgccgg
     3421 agttgttcgc ttcaggttac caatttctct cgcgggagga agtgcaacag tttgctgagc
     3481 cggttccaga cggccagacg gtacgttgtc tgatcgacat cgcgaaacgc cgcaagatgc
     3541 atcttgtg
//
LOCUS       DATMTI010000936         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_842246, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000936 DATMTI010000000
VERSION     DATMTI010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842246"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(514..1068)
                     /locus_tag="VN039_14755"
     CDS             complement(514..1068)
                     /locus_tag="VN039_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47257.1"
                     /translation="MYQTPIDTAMMSERVPQNVRRLAVWYPRTFEQDMTYGYSRLEQA
                     TFQLKKQRSWMKIVERRDIGQLTDEQRWQLSGRVGDDSVVRIGRWLGADSMVLFRIEG
                     PTMRERMLARFYGKMPPFVVSSKIISVESGEVLYHDIVTAMAVPESGEWDDYASDYEL
                     QPAMHAALDHALSVAIVHLNQSFR"
     gene            complement(1339..2220)
                     /locus_tag="VN039_14760"
     CDS             complement(1339..2220)
                     /locus_tag="VN039_14760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWV47258.1"
                     /translation="MRIATWNVNSLKARQEKVMWWLDRAKPDVLLMQETKLTDADAPV
                     TTFKQVGYELVHHGEGRWNGVAIASRCGISGVTTNFGEPLRQAQTSDTGDDEPLAEAR
                     MVAAECGGVRIICVYAPNGRLVGSPFYEAKLVWFDKLVRWINQAITPDTPTVIGGDFN
                     VAPEEIDVWDPSAYEGGTHVSERERQAYARLLNEGFVDAYRLHHPEPGRYTWWDYRAG
                     SFYKNFGMRIDHLLVTTPLRHRVVWAEIDREARKGKPLPSDHAPLVIDIYTPGHPFDA
                     GWASADSRIAARSPKQR"
     gene            complement(3032..3340)
                     /locus_tag="VN039_14765"
     CDS             complement(3032..3340)
                     /locus_tag="VN039_14765"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWV47259.1"
                     /translation="MPTYVSLVKFSQQGLQTMKDKGVERAEMVKKNAQALGGKLVQAY
                     YCLGEYDVVAIWDFPDNKTAMKAAVMNASMGHIQITTMPAVNRDEWKSLLQETMGRKK
                     "
BASE COUNT          799 a          978 c          944 g          813 t
ORIGIN      
        1 aaaactaggg taatccccaa tcgtgacagg gaaatgtcat caagagataa aaaggggaca
       61 ggctgatttg tttgaaccat ccgatacatc agagccccgc cggaccctgc ggctcatcgc
      121 tacttcccca ggcacgtagc aattcgagac tccgccgagg cccagccggc atcgaacgga
      181 tggccggggc tgtcaatgtc gatgatcagt ggtgcgtgac ccgatggtag aggttttcct
      241 tttccgcctt tcgatcgatt tccgccaaga caacacggta ttttaacgcc gtcgttacga
      301 gcatgtgatt gatgcgcatg ccgatgtttt aatgggacga aataaggggg tcggacaaca
      361 tagcgcaggt tttgtatctt gccgacgccg gctgcaggat gccgccggac agtcatgatt
      421 ctgcactgtg cactgcacca acaaactggt tgccaagctc ccatccgagt agttcaagag
      481 gaaaaacgcg ggggttaagg cccggcgtgt ttctcaccga aatgattggt taagatgcac
      541 aattgcgacg gacagtgcat ggtcgagagc agcatgcatc gccggttgca attcgtaatc
      601 gctcgcgtag tcgtcccact cgccggattc gggaaccgcc atggcagtga cgatatcgtg
      661 gtacagcact tctccgcttt ccactgaaat aatcttactc gataccacaa acggcggcat
      721 cttcccgtaa aagcgtgcga gcatccgctc cctcatagtg gggccttcga ttcggaacag
      781 aaccatgctg tccgctccga gccaccttcc gattcgcacc acactatcat cgccgacgcg
      841 tcctgagagc tgccatcgct gttcatccgt caattggccg atgtctcgcc gttcgacgat
      901 cttcatccat gaccgctgct ttttgagctg aaacgtcgct tgctcaagcc tgctgtatcc
      961 gtacgtcatg tcttgctcga acgtgcgtgg ataccacacg gctaaacgcc ggacattctg
     1021 cggcacccgt tcgctcatca tggccgtatc gatcggtgtt tgatacataa ctgcacgagg
     1081 cgcaggacga catcctgttt gcgaccaaag actgatcacc gcgacagtgg cagcgaggga
     1141 taccaaccca tttgtgcgag gtctgacagg catgcaaaca gagtatgaga gaagagggaa
     1201 aagccgctat cctacaaata ggggaaaatt gctactaagg aaaggagtag gaagaggaat
     1261 tgtggtctga agaagaggga cgacaagcac gaaaggatat cacgagccat ccgatgcatc
     1321 agagttcgcc cttgtggctc atcgctgctt cggcgagcgc gccgcaattc gcgaatccgc
     1381 cgaagcccag ccggcatcga atggatggcc gggggtatag atgtcaatga ccaacggtgc
     1441 gtgatccgag ggtaacggtt ttcccttccg tgcctcgcga tcgatctcgg cccagactac
     1501 acgatgcctt aacggcgttg tcacgagtag atgatcgatg cgcatgccga agtttttata
     1561 gaagctcccg gcgcggtagt cccaccacgt gtatcgacct ggttccggat gatgcaatcg
     1621 gtaggcatcg acaaatccct cgttcaacag tcgcgcatat gcctgccgct cgcgctccga
     1681 aacgtgcgta ccgccctcat aagccgaggg gtcccacaca tcgatctcct caggcgctac
     1741 attgaagtca ccaccgatga ctgtcggtgt atcgggagtg atggcctgat tgatccagcg
     1801 aaccagtttg tcgaaccaga cgagcttcgc ttcataaaac ggcgaaccca cgaggcgacc
     1861 gttcggcgcg tacacacaga tgatgcgtac gccgccgcat tcggcagcaa ccatcctcgc
     1921 ctcggccagt ggttcatcat caccggtatc agatgtctgc gcttgtcgta acggctctcc
     1981 aaaattcgtg gtaacgccgc taatgccgca tcgactcgcg atcgcaacgc cgttccatcg
     2041 accctcgcca tggtgcacca gctcgtatcc tacctgcttg aatgttgtga caggcgcgtc
     2101 cgcgtccgtc agcttcgttt cctgcatcag cagcacatcc ggcttcgccc gatccagcca
     2161 ccacatgacc ttttcctggc gagccttcag cgagttgaca ttccaggtgg caatccgcat
     2221 tcctaaaggt tatcccatct gagcagagtc tggagggaaa aaaacggtga cattctatta
     2281 ttctaagcgt gactcgggta acaagagacc ttcctgagac ccgtgctccg gaagaccgac
     2341 gtctgctggc ttataaaaag agcctgacga tcttgcgatc gtcgggccct ttaccgttgg
     2401 ctctgatggc actgactgct ttccgtgaga aagacagtca gtgtgaccgc agcttcatag
     2461 tatgagatct gtcgcttcag tcaatcaggc tcacccgtat cagtacctgg cgatgccaag
     2521 catcttcgtg agcagagcaa tgaccgtgac cagaaccccg atgccgataa acccatagat
     2581 cattattttt atatggtcca tgtgcatctc cttgtgatta gagttgacaa gaatttattg
     2641 acgacgacct tggcatcgtg caggactgca agtgagataa ggccttggaa aatgagctgc
     2701 gagtgatagc ttggtaccgc tcggtctttc tcatgctcaa ctccaggctg cccgcgtgac
     2761 tcatctgctg ttgagtttga ctccctgttc tgcgcgacgt ctctgagatt cccgtacctc
     2821 gggtgttcag cgggcaagag gcgaaagact acccataccg taggaatcac caggatgagg
     2881 aatcgaaacg cagtcgcgca gttcatgtcg atctccggga aggcagatag aattatgata
     2941 agccagtaag gctcagactt ccagatcagc ttggcggatc agtgtgtgcc attgcactgt
     3001 gcgaagatcc gagcgggcgt gttctgactg gctatttttt ccttcccatc gtttcttgta
     3061 ggagtgattt ccattcgtcc cgattgacgg ccggcatcgt cgtgatctgg atgtgcccca
     3121 tggaggcatt catgaccgcc gctttcatag ccgtcttgtt atccggaaaa tcccagattg
     3181 ccacaacatc gtattcaccg aggcaataat aagcctggac cagcttaccg ccaagcgctt
     3241 gggcgttctt cttgaccatc tcggcgcgct cgacgccctt atccttcatg gtttgcagac
     3301 cctgctggct gaacttcacg agactcacgt aggtcggcat ggcaaccctc cttggtggga
     3361 tggagacgcg tggattgttg gatgtcgtga aatcttaggc tcctttggtg ggccgaaaca
     3421 agagcgagtt tgccactcat tcgatggtgc catgaaacgg tgatcttgac tgctgagtgg
     3481 agaggggcgg cacggtgtcg atttctcgag gctcgttcga ctacaaggta agaa
//
LOCUS       DATMTI010000937         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_544592, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000937 DATMTI010000000
VERSION     DATMTI010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544592"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VN039_14770"
     CDS             <1..849
                     /locus_tag="VN039_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWV47260.1"
                     /translation="LEVIHSGRDKFAPEERITDAGGRRRWLQTIKRPIFDDQGRVHMV
                     LGAATDITERKRMEEILLQRERDLSAALQERERISQDLHDGILQSLYAVGLGLEACKP
                     LIKKQQDHVTKKFMTTLEQAIGQLNQVMTEIRNFIAGLESQVMQGGDFSTALRTMVET
                     MSVSCSAKCRVKIDDAAARRLSTEQALHIINIVREGLSNALRHSRAKQITVSLRDLVR
                     SDRLAVTDDGIGFNARSAQGVGHGLVNMAARAQKVRGLFAIQSKPDKGTRISLDLPKD
                     THYAHN"
     gene            836..1492
                     /locus_tag="VN039_14775"
     CDS             836..1492
                     /locus_tag="VN039_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV47261.1"
                     /translation="MLTTKTQAIRVLLVDDHEVIRVGLRTVLGQTQGVTVVGEAGTMA
                     EAIQQAQRLKPDVILMDVRLPDGSGVDACREILGTLPGTRVIFLTSYADDDSVLAAVL
                     AGAHGYVLKEIDSPALLEAIRSVAKGQSILDSSVTERALRWLRGLHDLPATPGTDQLS
                     SQEERVVALVAEGKTNKEIAVALGLSDKTVKNYLANVFQKLRITRRAQAAAFFVKRQV
                     "
     gene            1662..>3757
                     /locus_tag="VN039_14780"
     CDS             1662..>3757
                     /locus_tag="VN039_14780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWV47262.1"
                     /translation="MTAGLFLGLLLFLSFFLWIVIRQRQENVLNSTVIAATNCSVLVT
                     DATLSQHPIVYVNPAFLLLTGYAEQQVIGQRTSILTGPDTDRTSMEKLAMALQDGWAC
                     RVRLCHYRKNGTSFWNDVSLTPVKDRLGRVTAMVWTMSEVTQPGAGLKAPDRIQDEGI
                     AARGQAEQVLRDSETRLDLAVQIGQVGFFEYDHRTDALSCSPILRDIYGVGTDEPASW
                     QRYLELVHPDDRNRVISAAQRARAATGNGRYEVEYRLVRPDGPMRHISLRSLTSFDGD
                     GSSRQPVRTIGAVVDVTDRKNVEARRRESASIEAISTFAGGIAHELNNGLTAVLGFSE
                     LALPLIPTESKAHRHIRQVVAATKKSRELIQQLLAFGRQNDQDRCPLLLHSLAKESIK
                     FLRPTIPLWIKFRAQIAQTTNPISANAVQMHEMIFNLVDNALRAMQNTGGILDVQLQN
                     REFVADQIMPSGRLLAGRYVCLSVRDTSEGMDPDRVGRLFTSFLMSRDVSGRQGTGLA
                     VVHGIVTAHGGTLAVESQIGAGTTVSVYLPALSACVPSATQVGDPLPHGHECILFVDD
                     ENSVVRLGTEVLEALGYYPVVCRTAAEALEVFQVEPKRFDLLITDRTMPGMSGNCLAR
                     ECRRFRSDLPVIFCSGSKGVHHLDDACSQGITECLPKPFTLYELAHAIRRTLDQSPAY
                     LESGGGLANSGREPSR"
BASE COUNT          838 a         1011 c         1080 g          828 t
ORIGIN      
        1 cttgaggtga ttcattccgg gagggataag ttcgcccctg aagaaaggat caccgatgca
       61 ggaggaagga ggcgctggtt gcagacgatc aagaggccga ttttcgatga ccaggggcga
      121 gtccatatgg tgcttggagc cgcgaccgat atcactgagc gtaaacgaat ggaagagata
      181 ctcctgcaac gcgagcgaga cttgagcgcc gcacttcaag aacgggaacg gatcagtcag
      241 gacctccacg acggtattct ccaatcctta tatgccgtcg ggctcgggct agaagcctgt
      301 aaacccctga tcaagaagca acaagatcat gtgactaaga aattcatgac gacactggag
      361 caagcgattg ggcaactcaa ccaagtcatg acggagatcc ggaatttcat tgccggactc
      421 gaatcccaag tgatgcaggg tggagacttc tcgaccgcct tgcggaccat ggtcgaaacg
      481 atgtccgtct cctgttccgc caaatgtcgg gtcaagatcg atgacgcggc agcacggcgg
      541 ctttccaccg aacaggcgct ccatattatc aatatcgtac gggaagggtt gagcaatgca
      601 ctgcgccaca gtcgcgccaa acagatcacc gtatcgctcc gggatctcgt ccgttcagat
      661 cgtctcgccg tcacggatga cggcatcggg tttaatgcac gctcggctca gggggttggg
      721 catgggttag tcaacatggc ggcacgagcc caaaaagtcc gcggcttgtt cgccatccag
      781 tcgaaacctg acaaggggac gaggatctca ctcgatcttc caaaggatac tcactatgct
      841 cacaactaaa actcaagcca ttcgagtact gctcgtcgac gatcatgagg tcattcgggt
      901 gggattgcgg acggtgctcg gccaaaccca gggtgtcacg gttgtagggg aagcgggtac
      961 catggcggag gcaatccaac aagcgcagag gttaaaacct gacgtgatct tgatggatgt
     1021 tcggttgccg gacgggtccg gtgtcgatgc ctgtcgtgag atcctaggga cgctcccggg
     1081 gacacgcgtc attttcctga cgtcctacgc cgacgacgac tccgttctcg ccgcggtgct
     1141 cgccggcgcc catggctatg tcctcaagga aatcgattca cccgccttgc tggaagcgat
     1201 ccgctccgtt gcaaagggcc aatcgatctt ggactccagt gtgaccgagc gggcgctgcg
     1261 gtggttgaga gggcttcacg atctgccggc gacccccggt acggatcaac tatcctccca
     1321 ggaagaacga gtcgtcgcac tggttgccga gggaaagacc aacaaggaaa tcgccgtcgc
     1381 gctcggactc agcgacaaga ctgtcaagaa ttatttggca aacgtattcc aaaaacttcg
     1441 catcacgcgg cgcgcgcagg cggccgcctt ttttgtgaaa cggcaggtgt gaaacggcag
     1501 ggacgagcga caacattcgg ctctactact tttaggtgca aaaatttaac tttgcggcta
     1561 tccttcatac ctgggccttc ctcttgcatt tcatgtaagt tcacatcaac tgattcgtat
     1621 tcttaaccac tccgtgtgtc cacaccggga ggttgtcccg catgactgct ggtctcttcc
     1681 tagggttgct attgttcctc agtttctttt tgtggatagt gattcgacag cgccaagaga
     1741 acgtcctgaa ttcaaccgtc atcgcagcga ctaattgtag cgtgctcgtg acggatgcca
     1801 cattgtccca gcatcccatc gtctatgtaa atccggcctt cctgttgctg accggctatg
     1861 ccgagcagca ggtgatcggt caaaggacgt cgatcttgac cggcccggat acagatcgga
     1921 cctcaatgga aaaacttgcg atggccctcc aagatggatg ggcctgtcgt gtacggttgt
     1981 gccattaccg caagaacggc acctcatttt ggaatgacgt atcgctgaca ccggtgaaag
     2041 accgtttggg tcgggtgacg gcgatggttt ggactatgag cgaagtgaca caacctggag
     2101 ctggcttaaa agcgcctgat aggatccagg acgaaggtat cgccgctcgt ggacaagccg
     2161 aacaggtgct ccgggatagc gagacgcgac ttgatcttgc cgtacagata ggacaggtcg
     2221 gattctttga atacgaccac cggactgatg ctctttcctg ttccccgatc ctgcgagaca
     2281 tctatggtgt cggtaccgat gagccggctt catggcagcg ctacctcgaa ctcgttcatc
     2341 ctgatgatcg gaatcgggta atttctgcgg cccaacgagc ccgtgctgcc acaggcaacg
     2401 gccggtatga ggtcgagtac cgtctcgtta gaccagacgg ccccatgcgg cacatcagtc
     2461 ttcgttctct gacttcgttc gatggcgatg gttcctcgag acagccggtc cgtacgatcg
     2521 gggcggtcgt cgatgtgact gatcggaaga acgtcgaggc acggcgacgc gagtcagcga
     2581 gcatagaagc gatcagcacc tttgccggag gcatcgccca cgaactgaat aacggtctta
     2641 cggctgtact tggcttcagc gagttggccc ttccgttgat tccgaccgag agcaaggcgc
     2701 atcgccacat cagacaagtc gtcgcggcga cgaaaaaatc cagggagctg atccagcagt
     2761 tgctggcttt cggtaggcag aacgatcaag atcgatgccc attattactc cattcgttgg
     2821 cgaaagaatc catcaagttc cttcgtccga cgatcccctt gtggattaaa ttcagagcgc
     2881 agattgcaca gacgactaac cctatttcgg ccaatgccgt gcagatgcat gagatgattt
     2941 tcaacctggt cgataacgcc cttcgtgcga tgcagaacac gggaggaatc ctcgatgttc
     3001 aacttcagaa ccgggagttt gtagcggatc agatcatgcc gtccggtcga ctcctcgctg
     3061 gacggtacgt ctgcctgagt gttcgggata caagcgaggg catggatcct gacagagtcg
     3121 gtcgattgtt tacctcgttt ttgatgtccc gagacgtcag cgggaggcag ggtacgggac
     3181 tagcggtcgt ccacggcatc gtcacggcgc atggcggtac cctggcggtt gagagccaaa
     3241 ttggcgccgg tacgacggta tcagtctatc tccccgctct ctcggcttgt gttccatcgg
     3301 cgactcaggt gggcgatccg cttccccacg gacacgaatg tatcttattc gtcgatgacg
     3361 aaaactcggt tgttcggtta ggaacagagg tgttggaagc cctcggctac tatccggtgg
     3421 tatgtaggac ggctgccgaa gcattggaag tctttcaagt cgagcccaaa cggttcgact
     3481 tgctgatcac tgatcggacg atgcccggca tgagtgggaa ttgtctagct cgagaatgtc
     3541 ggcgatttcg atcggatctc ccggtcatct tctgcagcgg atcaaagggt gtacatcatc
     3601 tggacgacgc ctgttcgcag ggtattacgg aatgcctccc aaaaccgttt acattgtacg
     3661 agctggcgca tgccatccgt cggacattgg accagtcacc tgcctatttg gaatctgggg
     3721 gtgggctggc caactccggc cgagaaccat cgaggat
//
LOCUS       DATMTI010000938         4899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_118866, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000938 DATMTI010000000
VERSION     DATMTI010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118866"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VN039_14785"
     CDS             complement(<1..195)
                     /locus_tag="VN039_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47263.1"
                     /translation="MKTASLLFSVVLAAIIPLLSGCFATLEQRWEAFDAKMRQEVGVK
                     TKDYYVREWGRPAKRAKAGDG"
     gene            complement(219..1070)
                     /locus_tag="VN039_14790"
     CDS             complement(219..1070)
                     /locus_tag="VN039_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HWV47264.1"
                     /translation="MDSPTSQRTPTATIDLDAYFKRIGYEGVRQPTLDTLMAIQLRHT
                     ETIAFENLNSLMGWPVRLDAESLQQKLVRDGRGGYCFEHNLLLKHALDALGFRVTGLA
                     ARVLWNMPEGAVPPRSHMLLLVDLDGRRYVVDAGFGGVTLTGPLRLEIDIEQTTPHEP
                     FRLVKTDEEFIEQVKIGDHWVSLYRFSLAEQLLPDYEMTNWYVSTHPDSRFVTGLLAA
                     RPAPDRRYGLLNNQLTVHHRAGGKDRHLITSVTDMRETLESAFQIVLPDAPELDSTLA
                     RLIANAP"
     gene            complement(1344..1724)
                     /locus_tag="VN039_14795"
     CDS             complement(1344..1724)
                     /locus_tag="VN039_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47265.1"
                     /translation="MNTTIQRNGRMQRIWWLTGIVGGLLLISLGLELLARQVFGQTDV
                     GTLLWALYLGFWSFVLGTTGFLLLAAQWLIEWRRFGRDTKAMNVPKEYPRLVSNSGAL
                     NQIRRKAEPCTQAHEPDMVTSSSA"
     gene            complement(1809..2750)
                     /locus_tag="VN039_14800"
     CDS             complement(1809..2750)
                     /locus_tag="VN039_14800"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV47266.1"
                     /translation="MKVFVAGSTGAIGRILVPQLVENGHEVVALVRDDRKAEALLAVG
                     ATVVIADALNKEELTAAIRKAEPEVIIHQLTALGHVGNFKKLDEEFVLTNRFRTEVTD
                     TLLEAARLVGARRFIAQSFCGWPFAREGGPIKTEQDPLDPNPPTSFAKTLAAIRHLED
                     TVRKTMDLQAFALRYGLFYGPGTGIAKDGPVAELIRKRMLPIVGNGAGIWSFIHVHDA
                     ARATVASISSDSPGIYNVVDNEPAPVTTWLPALADAVGAKPPYRVPVWLGKLAIGEGG
                     VSMMTQIRGGSNAKAKRELGWEPTYQTWRRGFVEGLG"
     gene            complement(2900..4816)
                     /locus_tag="VN039_14805"
     CDS             complement(2900..4816)
                     /locus_tag="VN039_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV47267.1"
                     /translation="MNRSIVIAALLGTLVLGFVAIIHAPSLGAQSQAPSDTVSPELAY
                     LKQVNQWRTPSDPQLLFLLMQQFANAGRHVEGIAYFEEALNRFASRLTDNQKAQYLVA
                     IASLRAGHAHDVFLFKRLGWVRDTLALLDEAKQLTKNEMFITQWMSGVVRAKIPGFFG
                     EHDTALADLLWCVEHVDAAPHRNWLREVYAQLATLYRRRGDAREAERYQTLIGAGTEN
                     DTPVFTTPFAEDPSNGHTFISRSIREVIPGTVYCLSGFEFTEYYFVVSADRQELIAID
                     AGTRPDTAQAAYEALRNQVPSLPPLTTVFVTHAHWDHVGGHRYFLSLNPAVRFYGRST
                     YQEELLDDALGNPAMLQRFFGKKFRLDDVLTYKPDVLIDGPRDVVIAETRFRLMPTSG
                     GETSDALLVHMPEEGVLFVGDILMPYFGAPFVPEGSVDGLLEAINQVDRLKPGHLLHG
                     HEPLTRLFSSTAMLNDLRIQLTWLRDEVLRAMKAGTERGVIQQANLIPPTLANSASDV
                     HLAYLVIRENMINRLFQQNSGYWQNGLRGLDMLTDADHGAALVDYFGISDSQLAAAAE
                     RMMKDGRHELAANYLRWAQTKLPESPRLNESRRLAYLKLMEKYQEFNPFKLIVYAGEI
                     NHPVVQINETAGPQ"
BASE COUNT         1066 a         1417 c         1352 g         1064 t
ORIGIN      
        1 accgtctccg gctttcgctc gcttcgccgg gcgcccccat tcgcgaacat aataatcttt
       61 cgtcttcacg ccgacctcct gccgcatctt cgcgtcaaat gcctcccagc gttgttccaa
      121 ggttgcgaaa catccggaga ggagcggtat gatggcagcc aaaacgacag aaaacagcaa
      181 gctcgcagtc ttcatggtac acctctagaa gttttgaatc atggcgcgtt cgctatcagc
      241 cgcgccagtg tgctgtcgag ttccggcgcg tccggcagaa cgatctgaaa cgcgctttca
      301 agtgtttccc gcatatcggt aacgctcgta atcaggtgtc gatctttacc gccggctcga
      361 tgatgcacgg tcagttggtt attgagcaac ccataacgtc gatccggtgc gggccgggcc
      421 gcaagcagcc cggtcacaaa ccgagaatcc gggtgggtgg atacgtacca atttgtcatc
      481 tcgtaatcag gcaacaattg ctcggccaag ctgaaccggt acaacgagac ccagtggtcg
      541 ccgattttga cttgctcgat aaattcctcg tcggtcttca ccaaacggaa cggttcatgc
      601 ggggtggttt gctcgatatc gatttcgagc cgcaatgggc ccgtcagcgt caccccaccg
      661 aatccagcgt ccacgacgta gcgtctgccg tcgagatcga ccagaagcaa catatgactc
      721 cggggcggca cggctccttc cggcatgttc cacaacacgc gtgcggcgag accggtcacg
      781 cggaacccca gcgcgtcgag cgcatgtttc agcaggagat tgtgctcgaa gcaataaccg
      841 ccgcggccgt cccgcaccag cttctgctga agcgactccg catccaagcg aaccggccat
      901 cccatcaagg aattcaggtt ttcaaacgcg atggtttcag tgtgccggag ctggatggcc
      961 atcagagtat ccagcgtcgg ctggcgtact ccctcgtaac cgatacgctt gaaataagca
     1021 tccaagtcga tcgtcgccgt cggagtccgc tgcgaggttg gactatccat gcatgctcct
     1081 gaaagtcggt acaacaccga gagtggtctt ggcatgacag aacgtgatca tgccgtactc
     1141 tctgcgcgat gcacccagaa tttcggcaat aagaggcttg ttgcaatact gtgggctaag
     1201 gcggagaacg gcagaaggag cgattgcatt ctcagcaaca ttttggaccc ctctgacctc
     1261 ctcctcgtct cgccacgcgg gacagccaca gaggctccct tgatacccgt gaccggctta
     1321 tgcgtggcca accgtacgct tcgttatgca gaagatgaag ttaccatatc cggctcgtgt
     1381 gcttgagtgc atggttccgc tttcctgcga atctggttaa gcgcccctga atttgacacg
     1441 agccgaggat attctttcgg tacattcatt gctttagtgt ctcgaccaaa acgtcgccac
     1501 tcgatcagcc actgcgctgc gagaagaagg aagccggtcg tgcccaggac aaaactccag
     1561 aaacccaaat agagcgccca gagcagagtg cccacatcgg tctgtccgaa cacttgacgc
     1621 gcaagcaact ccaatccgag gctgatgagg agcagcccgc cgacaatgcc ggtaagccac
     1681 cagatacgtt gcattcttcc gtttctttga atcgtggtgt tcataagatc accgtttcta
     1741 tgattattgg atggatttcc ggttcagaaa ccgtcctttc gatatgtcca ttcgcgtgac
     1801 gaaagacgct agccaagccc ttcgacgaaa ccccgtcgcc aggtctggta cgtcggttcc
     1861 catcccaact cacgtttcgc cttggcgttc gatccgccac ggatctgggt catcatcgat
     1921 acgccgccct cgccgatggc cagcttgccg agccaaaccg gcactcgata gggcggcttt
     1981 gcaccgacgg cgtcggcaag cgccggaagc cacgtggtga cgggagccgg ctcgttgtcc
     2041 accacattat agatccctgg actatcgctc gaaatggagg caaccgtcgc gcgcgccgcg
     2101 tcatgaacgt ggatgaatga ccagataccg gccccgttgc cgacgatcgg taacatccgc
     2161 tttcggatga gttcggcaac aggcccatcc ttcgcgatcc cggtgccggg tccgtagaag
     2221 agtccgtaac gcaaggcgaa cgcctggagg tccatggtct ttcggactgt atcttccaaa
     2281 tgccggattg ccgccaaggt cttggcaaag ctcgtaggtg ggttcggatc aagtgggtcc
     2341 tgttccgtct tgatcggtcc gccttcccgc gcgaacggcc acccgcagaa gctctgtgcg
     2401 ataaaccggc gggctcccac cagacgcgct gcttcgagta gggtgtccgt gacttcagta
     2461 cgaaatcggt tggtcagtac aaactcctcg tcaagcttct taaagttgcc gacatggcca
     2521 agggccgtca gctgatgaat gattacttcc ggctcggcct tcctgatggc cgccgtcaac
     2581 tcctccttgt tgagcgcgtc ggctatgaca accgttgcgc ccacggccag gagagcctcg
     2641 gcctttcgat cgtcacgaac gagagcgacc acctcatgcc cattctcaac cagttgcggc
     2701 acaaggatcc taccgatggc tccggtactg cccgcgacaa atactttcat cgtaatggct
     2761 cctcatctgc aacacctctc aacgcggaga gcatcgcatc aacaaacaag ccactcgatc
     2821 gtcgagggcg cccaaaaaat ttgtcaggac tcgctttgat tcccacacga tgaagaatgt
     2881 cagccatggc acatctcctt tactgtggcc ctgccgtctc atttatttgc acgacagggt
     2941 gattaatctc gccagcgtag acaatcaact tgaacgggtt gaattcctgg tacttctcca
     3001 tgagcttgag gtaagccagt cggcgagact cgttcaatcg aggactctcg ggtaactttg
     3061 tctgtgccca tcggaggtag tttgcggcca gttcatgcct gccgtccttc atcatgcgct
     3121 cggccgcggc ggcaagctgt gagtcggaga tcccgaagta gtccaccagc gctgcgccat
     3181 ggtccgcgtc cgtcagcatg tccaatcctc gcaagccgtt ttgccagtaa ccgctgttct
     3241 gctggaacag acgattgatc atgttttctc ggatcacaag ataggcgagg tgtacgtccg
     3301 atgcactgtt cgcaagcgtg ggtgggatca agttggcttg ttggatcacg ccacgttccg
     3361 taccagcctt catggcacgc aacacttcat cgcgcagcca tgtcagctga atgcgtaagt
     3421 cgttcagcat agccgtggat gagaagagtc gcgtcagcgg ctcatgtccg tgtagcaaat
     3481 gcccgggctt cagccggtcg acctggttta tagcctccaa gagaccgtcg acgcttccct
     3541 ctggaacgaa cggggcccca aaatacggca tcagaatgtc gcccacgaaa agtacgccct
     3601 cctccggcat atgcacgagg agggcatcgg aggtttctcc accgcttgtc ggcatcagac
     3661 gaaagcgagt ctcggcgatt accacatcac gtggaccgtc gatcaggaca tccggcttgt
     3721 aggtgagcac gtcgtcgagt ctgaatttct ttccgaagaa acgttgcagc atcgcaggat
     3781 tgcccaacgc gtcatccaag agctcttctt gataggtgct tcgaccatag aagcgcacgg
     3841 ctggattgag gctgaggaaa taccgatgcc cgcccacgtg gtcccagtgc gcgtgcgtga
     3901 cgaagaccgt tgtcagagga gggagtgatg gaacttggtt tcgtagcgcc tcataggctg
     3961 cctgggcggt gtctgggcga gttccggcat caatggcgat caattcctgc cgatccgccg
     4021 agaccacaaa gtagtattcg gtgaactcga agccggacag gcaatacact gtgccgggaa
     4081 taacttctcg tatggagcgt gatatgaacg tgtggccatt ggacggatct tcggcgaagg
     4141 gggtggtgaa caccggcgtg tcgttctcgg tgccggcgcc gatcagagtt tggtaccgct
     4201 ccgcctctct ggcgtcacct cgtcggcgat acagggtggc caattgggcg tagacctcgc
     4261 gcagccaatt tcggtgcggg gcggcgtcca cgtgttcgac acaccagagc aagtcggcca
     4321 gcgcggtatc gtgttcgcca aaaaaaccag gaatcttcgc tctgactaca ccggacatcc
     4381 attgcgtgat gaacatctcg ttcttcgtga gttgcttcgc ttcgtcaagc agagccaacg
     4441 tgtcgcgcac ccagccaagc cgtttgaata gaaagacatc gtgagcatga ccggcgcgaa
     4501 gggaggcaat tgccacaagg tactgagcct tttggttgtc ggtcagcctg gaggcaaaac
     4561 gattcaaggc ttcttcaaaa tatgcgatgc cctcgacatg acggccggca ttggcgaact
     4621 gctgcatcaa taggaaaagt aattgagggt cggagggtgt cctccattga ttgacctgtt
     4681 tcaagtaggc cagttcggga gagacggtat cagagggcgc ttgcgattgg gcacccagtg
     4741 atggagcgtg aatgatggcg acgaaaccta aaaccaaggt ccccagaagg gctgctatca
     4801 caatggagcg attcatgggg ctctcctttt tgtcctagat atagcaccct ctacagtcgc
     4861 gatcacattg ggaatgcaca catttaaaat agtcgagga
//
LOCUS       DATMTI010000939         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_799260, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000939 DATMTI010000000
VERSION     DATMTI010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799260"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VN039_14810"
     CDS             complement(<1..1273)
                     /locus_tag="VN039_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47268.1"
                     /translation="MRIRYNHKVNKLQPMLSVHFPLCRHVISFYVVLALALSGIGCSL
                     LKRGKEPVSSQPIPLTPVIWFSPSVTTAEVPYLDGCGETRSVTLTDALVASVPKKLTD
                     VFTAVTAQSQADETIASDGVIEVGVGLRRIELAVPKQAQGIYPATATVGIEMVFLARD
                     GALLFSKKLEGTGRGTVTVGEQPCQIAGVEVIVQDAMDSAITGLAQQIAQSVQIREYA
                     AQRGAEVPTAARPHSSVAAPTAETATSTPVMETAAQQLSVASTAPAVPAQLSFRAIIR
                     DENRDQFLQPDESITIELEVKNEGMGEAKDVAVTAEGKAELTAFFPAEVQIGSLKPGE
                     IKRTSISQRVTAPQEHLHGELTLNLRTTSPVVSVHPPKVFSFGVKPKVIDPALVPDVD
                     NMPNSLAAFTQPKAVIISIGVGTFRDGQVPAI"
     gene            1334..>1811
                     /locus_tag="VN039_14815"
     CDS             1334..>1811
                     /locus_tag="VN039_14815"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWV47269.1"
                     /translation="MSCPAVSSSASWGIPWPALARLIRLPNQTGTYLLLLPTMWGLVL
                     AARGIPPLHLVAIFIGGSFLMRSAGVIMNDLADQAFDRQVTRTKTRPLASGELSRRHA
                     AILLAVLLVVAASLLFFLQPIVTWLAPVAVFLAALYPYSKRWIHIPQAMLGIAFGWG"
BASE COUNT          335 a          558 c          501 g          417 t
ORIGIN      
        1 tgatcgccgg cacctgtccg tcacgaaacg tcccgacgcc gatcgaaatg atgacggctt
       61 tcggctgagt aaacgccgcc aaagaattcg gcatgttgtc gacatccgga accaacgccg
      121 ggtcgatgac tttaggtttg accccaaaac taaatacctt gggcgggtga acggacacca
      181 ccggactggt cgtccgcaag ttcaacgtca attctccgtg aagatgttct tggggcgccg
      241 tcacccgttg gctgatcgag gtgcgtttga tctctcccgg cttgaggctg ccgatttgaa
      301 cttccgcagg aaagaaagcc gtcagctcgg cttttccttc cgctgtgacc gccacgtctt
      361 ttgcttctcc cattccctcg ttcttcactt cgagttcgat cgtgatggat tcatccggct
      421 gaaggaattg gtcccgattt tcatcccgga tgatggcgcg aaaactcagt tgcgccggca
      481 cagccggcgc ggtcgaagcg accgacagtt gttgcgccgc cgtctccatc accggggtgg
      541 acgtggcggt ctccgctgtc ggcgccgcca cagaggagtg tggacgtgcc gccgttggaa
      601 cctcggcgcc tctttgtgcg gcatattctc ggatctgcac cgattgcgcg atctgttgag
      661 ccaagccggt gatagccgag tccatggcgt cctgaacaat cacctccacg cccgctatct
      721 gacacggctg ttccccgact gtcaccgtac cacgcccggt accttcgagc ttcttactga
      781 agagtaacgc tccgtccctg gccaagaaga ccatctcaat tcctaccgtc gccgtcgccg
      841 gatagattcc ttgcgcttgc ttggggacgg caagctcgat ccgcctcagt cctaccccta
      901 cctcgatcac gccgtcggac gcaatggttt catcagcctg actctgagcc gtgaccgcgg
      961 taaagacgtc cgtcaacttc ttgggaacgg aggcgacaag agcgtcagtc agcgtaaccg
     1021 atctcgtttc gccgcaacca tcgaggtatg gcacctcggc tgtggtcacg ctgggtgaga
     1081 accagatcac cggcgtcaat ggaatcggtt gggaactgac cggctccttc ccccgcttga
     1141 gtaaggaaca accgatacct gacaaggcta gtgccagcac cacatagaat gagatgacat
     1201 gacggcatag tgggaaatgg acgctcagca tgggttgcag cttgttcacc ttgtggttat
     1261 accgaattcg cataggcgtc acaaccggcg aagacgcatt gacagcctgt ttcctgtggg
     1321 ctaaggtcgc gtcatgtcct gtcccgcagt gtcatcttca gcgtcctggg ggattccgtg
     1381 gcctgcgctt gcccgattga tccgtctgcc gaatcagacc ggtacttatc tcttgctcct
     1441 tcccacgatg tgggggctgg tgctcgccgc ccgggggatt cccccgctcc atctagtagc
     1501 cattttcatc ggcggatcgt tcttaatgcg gagcgcggga gtgatcatga acgatttggc
     1561 ggatcaagcg ttcgaccggc aggtcactcg taccaaaact cgtcccttgg cctcaggtga
     1621 gttatcgcgg cgccatgcgg ctatactact ggccgtgctc cttgtcgtgg cggcaagcct
     1681 cctgtttttc cttcaaccga tcgtgacctg gctcgccccg gttgcggtct ttcttgcggc
     1741 cctctacccc tactccaaac gatggatcca tatcccacaa gccatgctgg gcatcgcctt
     1801 cgggtgggga a
//
LOCUS       DATMTI010000940        10344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_289040, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000940 DATMTI010000000
VERSION     DATMTI010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289040"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VN039_14820"
     CDS             complement(<1..1592)
                     /locus_tag="VN039_14820"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV47270.1"
                     /translation="MILLLLFSLMTVPATALAEPVIDSSAVQAQSTRTAPLTIEEVLA
                     RIELTHPLLRATGLERTQARAKVLKALGAWEPKLRNELEFDRFETYNLTNVSGAPNKL
                     SVGYNDTYLKVGHPWGWEIFGGIRSGFGDRGTIVGSGNTLIPTHVPISPAVAIPQDLQ
                     LFYQQQSAIVGGAFDLLRGFMVNEAFAGLQQAEIAGPQAEVKVAQKRQDLYLAGAVQY
                     WDWQVAVKQADVVKRALAVAEERYRMVEGRAKAGAVAPIDVVEAREEVQRRREAAIAA
                     QRKVEYEQYKLALFLWENGEPVTPRPEWAPEFQGETPLPSEADVAAFKVEAAEDRPEV
                     RDLYIEAKLNLVELKLAKNNLLPKLTLEGGPQTGAIYVFGGFAYKVGVFFSMPLFNRA
                     ARGKVLHAEAEQERLAYKQAYTERQVGIDVDNWLSAMVRARDRVKASTEALRLAKTLE
                     EGERARFNMGATSVLFVNIRERAVVEMAYELYRAQADYAVSRGGMLWARGALSKPLAD
                     NVLAKYGDPLHAAGSSGRKLPGR"
     gene            complement(1618..3171)
                     /locus_tag="VN039_14825"
     CDS             complement(1618..3171)
                     /locus_tag="VN039_14825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV47271.1"
                     /translation="MAALLLLLLILILLPVTTLAEPVIDSRAVQAQSTRTEPLTISEV
                     LARIELTHPLLRATGVERMEARAKILKALGAWEPIFKNETEAGRYQVWNFLQFINENT
                     GGFNDSLLEVGHPWGFRIEGGIRNGFGDRLSQNSNALLANDGLLFWPQQQALFAGEAH
                     LLRGFMMNEEYAAFQKAELTGPQAEIAVAQKRQDLYLAGAVQYWDWQVAVKQAEIVKR
                     TLAVAEERFTQIEGQAKRGLASPLDVIEANQEVQRRREAAIAAQRKVEYEQYKLSLFL
                     WEGGEPVTPRPEWAPEFQGETPLPTKEEVAAYKVEAKEDRPEVRELYIEAKMNNIDIK
                     LAKNHLLPKLDFKGGQIPFALDWLVGIGYRFEADFEMPLFQRKGRGEVMYAEADQQQL
                     VMKQLYTEQQVSVDVDNWLSAQVRARDRVKAATEALRLAKTLEEGERTRFNMGASTVL
                     FVNVRERNVVEQAYQLYRAQAEYAVSRGGMLWARGALSKPWPQSELAKYGNPLAAAGM
                     NGYKRPGRD"
     gene            complement(3195..4748)
                     /locus_tag="VN039_14830"
     CDS             complement(3195..4748)
                     /locus_tag="VN039_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV47272.1"
                     /translation="MIALVLAIVTLLPMTALAEPVIDSRAVQAQSTRTAPLTIGEVLA
                     RIELTHPLLRGMGAERAKARAKILKALGAWEPTVKSYSQTERYNTWNLTTAFDIPSQH
                     TGGYNDSTIQVGHPWGFRVEGGLRSGYGDRGNNNLIAFPSELLATYHTQHFYYGASFH
                     LLRGLMVNEENAQFQQAELAGPQAEIKVAQKRQDLYLAGAVQYWDWQVAVKQADVVKR
                     ALAVAEERLVQVEGLAKGGKVAPLDVVEANQEVQRRQQAAITAQRKVEYEQYKLSLFL
                     WENGEPVTPRPEWAPEFQGETPLPTNDEVAAYKVEAKEARPEVRDLYIEAKLNNIDIK
                     LAKNKLLPKLDFEGAPQPNFADWMVGYQYRLGLHAEMPLFQREARGKVMAAEVSQQQL
                     ALKQQYTEQQVTFDVDNWVSAIVRSRDRVTAATEALRLAKTLEEGERTRFNMGATTVL
                     FVNLRERHVVEQAYQLYRAQADYAVARGGMLWARGALSKPWPTESLAKYGNPMTAAGM
                     NGYKQPGRD"
     gene            4891..5790
                     /gene="nhaR"
                     /locus_tag="VN039_14835"
     CDS             4891..5790
                     /gene="nhaR"
                     /locus_tag="VN039_14835"
                     /inference="COORDINATES: protein motif:HMM:NF008284.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HWV47273.1"
                     /translation="MDWLNYHHLLYFWSVARHGTVTKACEELRLAQPTISGQIHLLEN
                     TLGEKLFIRTGRRLALTEMGQLVFKYAEDIFTTGQELMNTVKGQGNGHPTQLVVGIAD
                     AVPKPLATQLLKSALKLYRPTRLICQEDKLRQLLTDLTAHRLDLVIADTPAPPSIKEH
                     TYSHPLGESGVTLFATAKLAAQYRRGFPQSLSGAPVLLPTSNAALRRLLDQWLVNYGV
                     QPNIVGEFDDSATLKAFGQDGHGIFPGASVIEKEICRQYRVQVVGQVEGLKQHFYAIT
                     VERRLKHPVVLALVRTARRELLS"
     gene            complement(5824..6537)
                     /locus_tag="VN039_14840"
     CDS             complement(5824..6537)
                     /locus_tag="VN039_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWV47274.1"
                     /translation="MARTIRINKFFTDHGICSRREADRLIESGVITINDRVAQLGDQV
                     VSTDVIARAGCVIPWGKSNLYIKYHKPVCVTTTSESHVPRNIIAEIGHRERIFPIGRL
                     DKDSSGLILLTNDGDIVNEILRAEFGHEREYLVQVDRPFDQSFLDHLSRGVIILGSRT
                     KPCRTMRMGRDQFRIILTEGRNRQIRRMCRTLGYRVITLHRTRIMHITVNGLSAGSWK
                     ELTSPEREELLRAVGRPQV"
     gene            complement(6574..7506)
                     /gene="speB"
                     /locus_tag="VN039_14845"
     CDS             complement(6574..7506)
                     /gene="speB"
                     /locus_tag="VN039_14845"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HWV47275.1"
                     /translation="MTLPVGWEGPDHNFLGIDEPWCHPDQAGVYVLPAPYEHTSSYIR
                     GSDRGPSAILQASAQVEFYDEQLACEPFREWGGIATASPLDLKGDVDRAAVDTIEAFV
                     SPHVGTGRFLITLTGEHTGALGAIRAHAKRYPQMTVVQIDAHGDLRDAYQGNPFSHAS
                     VMARVVEDGLSLVQVGIRSISPEEVARIDATVRIKTFFAATILDPSGPYEGKASNWIP
                     DVIAACPTPVYLTFDCDGLDASIVPALGTPEPGGLGWYDTLNLITALANGPGIVGMDV
                     SEIAPIEGFVAPQFSIARLIYRILGRIRVGRRVH"
     gene            complement(7573..8145)
                     /locus_tag="VN039_14850"
     CDS             complement(7573..8145)
                     /locus_tag="VN039_14850"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HWV47276.1"
                     /translation="MMVPTHMFLTRGVGVHKEKLASFEEALRSAGVAYCNLVSVSSIL
                     PPHCKIIPRKRGEKLLKPGEITFCVMARSETNERNRLISASIGLAKPTDHGTYGYLSE
                     HHAHGETDEETGEYTEDLAAQMLATTLGVEFDPNVAWKEREQVFKMGGKIVRTLNITQ
                     SAVGKKGKWTTVIALAVFIPPENVPNRSRK"
     gene            complement(8299..9753)
                     /locus_tag="VN039_14855"
     CDS             complement(8299..9753)
                     /locus_tag="VN039_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWV47277.1"
                     /translation="MQESRPFLVHGQWKSGEFSASVVDPFTGKLVAQVIQATESDVEE
                     AIASTCSAAATMEQLPGHARYNILQQVAALLYRRRDEFVQAITAEAGKPITDAKREVG
                     RAIQTFTVAAEEARRIPGEVVPLDWTPGFDTHLGVLRRFPIGPILGITPFNFPLNLVA
                     HKVAPALASGNPILIKPAPQTPLTALLLGEVAMEAGIPPGGLNVVPCDNILAERMVVD
                     PRFKLLSFTGSASVGWMLKAKCGKKKVTLELGGNAGVVIEPDADLELAAQRCAAGGFG
                     YAGQTCISVQRVFVHHSIADTFTTKLLMHVARLKVGDPADEATSIGPLIDQAAAQRVE
                     SWIGEAVADGARVLLGGKRMGSLVEATVLSNVNPDMKVSCKEVFGPVVTVTPYRQISE
                     AVALLNQSDYGLQAGIFTQDINKIFYAFRHLEVGAVLANEIPTFRADHMPYGGVKDSG
                     LGREGVCAAIEDMTEPRMLIMNLKDPPYSPEKSV"
     gene            complement(9789..10061)
                     /locus_tag="VN039_14860"
     CDS             complement(9789..10061)
                     /locus_tag="VN039_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47278.1"
                     /translation="MASMQSFWPVSHRDDDFWVCMSCLTEVFYRKVPMPDCPSCHGVS
                     TYEGFTLEAIRDWGTEELIAKAVVAQEAATVAQPAAEATTPVEATD"
     gene            10062..>10344
                     /locus_tag="VN039_14865"
     CDS             10062..>10344
                     /locus_tag="VN039_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47279.1"
                     /translation="MDTLIFSDEEDYRTCVRDVNITRNGSFCLGAIQLFFVARQKSID
                     FFCEHLEWKGSRDRNGRLFFRRLGGRSHENEPRGSMKPPLIGFLAVFENR"
BASE COUNT         2050 a         3255 c         2814 g         2225 t
ORIGIN      
        1 cgtcccggta gtttacgacc ggacgagccg gctgcatgca gtggatcccc atacttggcc
       61 agcacattgt cggctagcgg cttcgatagc gccccccttg cccaaagcat tccgcctcgg
      121 gacaccgcat aatcggcttg agctcggtag agctcgtaag ccatttcgac caccgcacgc
      181 tctcgtatgt tgacgaagag cacgctcgtt gctcccatat taaaacgggc tcgttcgcct
      241 tcttccaagg ttttggccaa gcgcagagcc tctgtcgagg ccttcacccg gtctcgggcc
      301 cgcaccatcg ctgagagcca gttatcaacg tcgattccga cctgtcgctc cgtgtaggct
      361 tgcttatagg ccaatcgttc ctgctcagcc tccgcgtgaa ggaccttccc ccgggcagcc
      421 cgattgaaca acggcatact gaagaaaaca cccactttgt aagcgaaccc tccgaaaaca
      481 tagatagcgc ccgtttgcgg tcccccctca agcgttagct tcggcagcag attattcttc
      541 gccagtttca gttcgacaag gttcaatttg gcttcaatgt agagatcgcg cacttctggg
      601 cggtcttccg cggcttccac cttgaaggcc gccacatccg cttcgctcgg caatggcgtt
      661 tccccttgaa attccggcgc ccattctggt cgcggcgtca ccggctcgcc gttttcccag
      721 agaaagagcg ccagcttata ttgctcatac tccaccttcc gttgcgcggc aatggcagcc
      781 tctcgacggc gctgaacttc ttctcgggct tcaaccacgt cgatcggagc gaccgcgccg
      841 gctttggctc tcccctcaac catgcgatag cgctcttccg cgacggccag cgcacgtttc
      901 accacatcgg cttgtttgac ggcgacctgc caatcccagt attgaacggc accggcgaga
      961 tagagatctt gccgtttctg ggccaccttc acctcggctt gcggcccggc gatctccgcc
     1021 tgttgcaacc cggcaaacgc ttcgttgacc atgaatcccc gcaggagatc aaacgctccg
     1081 ccgacgatgg ccgattgctg ctgatagaac agttgcaggt cttgggggat cgccaccgca
     1141 ggactgattg ggacatgagt cggaattagg gtgttgccgc tcccaacgat agtgcctcta
     1201 tccccaaagc cgctgcggat accgccgaag atttcccacc cccaggggtg cccgaccttg
     1261 agataggtat cgttatagcc aacactcaat ttattcggtg cgcctgaaac gttcgtcaga
     1321 ttatatgttt caaaccggtc gaactcgagc tcgttccgca gcttcggctc ccaggcaccc
     1381 aacgctttca ggacttttgc ccgagcctgc gtccgctcca gtcctgttgc ccgaagcagc
     1441 ggatgggtca attcaatcct ggccagcacc tcttcaatgg tcaggggagc ggtgcgagtc
     1501 gattgagcct gcacggcact cgagtcgatc accggttccg ccagtgcggt cgccggcaca
     1561 gtcatcagcg agaacagcaa gagaaggatc aaggcaggat gctcctttgt tgctgaatta
     1621 atcgcgtccc ggtcgcttgt agccgttcat gcccgccgcc gccagcgggt tcccgtactt
     1681 agccaactcg ctctggggcc acggcttcga caacgcgccc ctggcccaca acatccctcc
     1741 acgcgacacg gcgtactcgg cctgcgcccg atacagctgg tacgcctgtt ccaccacgtt
     1801 gcgctcacgg acgttcacga agaggacggt gctcgcgccc atattgaatc gggtccgttc
     1861 gccctcctcc agcgtcttgg ccaaccgcag cgcttccgtg gccgccttca cccggtctct
     1921 ggcccgcact tgggccgaga gccagttgtc cacgtcgacg ctgacttgtt gctcggtgta
     1981 cagctgtttc atgaccaatt gttgctgatc cgcttccgca tacatgacct ccccacgccc
     2041 tttccgctgg aacagcggca tctcaaagtc cgcttcgaac cgatacccga ttcccacaag
     2101 ccagtccaag gcgaacggga tttgcccccc cttaaaatcc aactttggga gcaggtggtt
     2161 cttggcgagc ttgatgtcga tattgttcat cttagcttcg atgtagagct cccgcacttc
     2221 cggccgatct tctttcgcct ccactttata ggccgcgacc tcttctttgg tcgggagcgg
     2281 cgtttccccc tggaactccg gcgcccattc cggtcgcggc gtcaccggct cgccgccctc
     2341 ccagagaaag agggacagct tgtactgctc atactccacc ttccgctggg cggcgatggc
     2401 tgcctctcgg cgtcgctgga cttcttgatt ggcttcgatt acatcgagcg gcgaggccag
     2461 cccgcgcttg gcctggccct cgatctgcgt gaagcgctct tccgcgaccg ccaaggtccg
     2521 cttcacgatc tcagcctgtt tgaccgcgac ctgccaatcc cagtactgca cggcgccggc
     2581 gagatagaga tcttgccgtt tctgcgccac cgcgatctcc gcctgcggcc ccgtgagttc
     2641 ggccttttga aacgcggcgt actcctcgtt catcatgaag ccgcgcagca ggtgggcctc
     2701 cccagcgaag agcgcttgtt gctgaggcca gaacagcagg ccatcgttcg cgagcaacgc
     2761 attggagttc tggctaagcc tatccccaaa gccgttccgg atcccgcctt cgatccggaa
     2821 gccccaggga tgccccacct caagcaggct gtcgttaaat ccccccgtat tctcatttat
     2881 gaattgcagg aaattccata cctgataccg acccgcctct gtctcgttct tgaagatcgg
     2941 ttcccaggct ccgagcgcct tcaggatctt cgcccgggcc tccatccgtt ccaccccggt
     3001 cgcccgaagc agcggatgcg tcaactcaat acgggcgagc acttcgctga tggtcagggg
     3061 ctcggtgcga gtcgactgag cctgcacggc acgcgagtcg atcaccggtt ccgccagtgt
     3121 cgtcacagga agcaagatca gaatcaggag taatagtaat aaagcggcca tcgacggatg
     3181 ctccttgtgc cggattaatc gcgccccggt tgtttatagc cgttcatgcc cgccgccgtc
     3241 atcggatttc catacttcgc caaggactcc gtcggccagg gcttcgacaa cgcgccccgt
     3301 gcccacaaca ttcctccacg cgccacggcg tagtcggcct gcgcccgata cagctggtac
     3361 gcctgttcca ccacgtggcg ctcacggagg ttcacgaata acacggtggt ggcccccatg
     3421 ttaaacctcg tgcgctcacc ttcctccaac gtcttggcca gccgcagggc ttccgtcgcc
     3481 gccgtcaccc ggtctctgga gcgcacgatc gccgagaccc agttgtccac gtcgaaggtg
     3541 acctgctgct cggtatattg ctgcttcaac gccaattgtt gctggctcac ttctgcggcc
     3601 atgaccttcc cacgcgcttc ccgctggaac agcggcatct cagcgtgcag gccgagccga
     3661 tactggtatc ccaccatcca gtccgcgaag ttaggttgcg gcgccccttc aaaatccaac
     3721 tttgggagca gtttgttctt ggcgagcttg atatcgatat tgttcagctt ggcttcgatg
     3781 tagaggtccc gcacttccgg ccgcgcttct ttcgcctcca ctttataggc cgcgacctcg
     3841 tcgttggtcg ggagcggcgt ttccccctgg aattccggcg cccattccgg tcgcggcgtc
     3901 accggctcgc cgttctccca gagaaagagg gacagcttgt actgctcgta ctccaccttt
     3961 cgttgcgcgg tgatcgcagc ctgttgccgc ctctgaacct cttggttcgc ttcgacgaca
     4021 tcgagcggcg cgaccttccc gcccttggcc aacccttcga cctggactag gcgctcttcc
     4081 gcgacggcta gcgcacgctt caccacatcg gcttgtttga cggcgacctg ccaatcccag
     4141 tactgcacgg cgccggcgag atagaggtct tgccgtttct gcgccacctt aatttccgct
     4201 tgcggccccg cgagttcagc ctgttgaaac tgggcgttct cctcgttgac catgaggcct
     4261 cgcagcaagt ggaacgacgc gccgtagtag aaatgttgcg tatgatacgt ggccagtaac
     4321 tcggagggaa acgcgatgag gttgttattg ccgcgatccc cgtagccact gcggagcccg
     4381 ccttctacgc ggaagcccca gggatgcccg acctggatcg tgctgtcgtt atatcccccc
     4441 gtatgttggc ttggaatgtc gaacgccgtc gtgagattcc acgtgttata acgttctgtc
     4501 tgactatagc tcttgaccgt cggttcccag gccccgagcg ccttcaggat cttcgcccgg
     4561 gccttcgctc gctcggctcc catcccccga agcagcggat gcgtcaactc gatgcgagcc
     4621 agcacttctc cgatggtcag tggagcggtg cgggtcgact gggcctgcac ggcacgcgag
     4681 tcgatcaccg gttccgccag tgcggtcatt ggcaggagag tcacaatggc caataccaga
     4741 gcaatcatga gggatgctcc tttcacatcg ggtggcggac ggcgcccaca gtatcaatct
     4801 cccgttcatt ttttaaaata gataaaacgg attggagtta tcggctaatc cgatgtatag
     4861 tgtctttcct cattcgaggc ctagggccat atggattggc tgaactatca ccacctgctg
     4921 tatttctggt ccgtcgccag gcacggcacc gtgacaaaag cctgcgaaga actccgcctc
     4981 gcacagccca ccatcagcgg acagatccac ctcttagaaa atacattggg cgagaagctg
     5041 ttcatccgaa caggtcgccg cctggccctg acggagatgg gccagctcgt gttcaagtac
     5101 gcggaggaca tcttcacgac cggtcaagag ctgatgaaca ccgtgaaggg gcagggcaat
     5161 ggacatccga cgcagctggt cgtcggcatt gcggacgcag tgccgaaacc gctggcgact
     5221 cagctcctca aatcggcttt gaagctgtat cggccgacgc ggttaatctg ccaggaagac
     5281 aagcttcgcc aactgctaac cgacctgaca gcccatcgat tggatctggt gattgccgac
     5341 acaccggccc cgccgagcat caaggaacat acctacagcc atcccttggg cgaatcggga
     5401 gtgacgctgt ttgcgacggc caagttggca gcacaatatc gtcgaggatt tccgcagtcg
     5461 ttaagcggcg cccctgtact ccttccgaca tcgaatgccg cgcttcggcg gctgctcgac
     5521 caatggctgg tcaactacgg agtgcaaccc aacattgtgg gggagttcga cgacagcgcg
     5581 accttgaaag cgttcggaca ggacgggcat gggatcttcc ccggcgcatc ggtgatcgag
     5641 aaggaaatct gtcgccaata tcgagtgcaa gtggtcggcc aagtggaagg gctcaagcag
     5701 cacttctatg ccatcaccgt ggagcggcgg ctgaagcacc cggtcgttct cgccctcgtc
     5761 cgaaccgccc gtcgcgagtt gctcagttag ccgtcaattg ggcttccact agaaaaaacg
     5821 cgatcagacc tgaggccgcc ctaccgctct caggagctcc tcccgctccg gactcgtgag
     5881 ttctttccaa ctcccggcgc tcagcccgtt gacagtaatg tgcatgatcc tcgttcggtg
     5941 caacgtaatc acccgataac ccagcgtccg gcacatgcgc ctgatctgcc gattgcgtcc
     6001 ctctgtcaga atgatgcgaa actggtcacg ccccatgcgc atcgtccgac agggcttcgt
     6061 ccggcttcca agaataataa caccccgcga cagatgatcc aaaaaggatt ggtcgaacgg
     6121 acgatcgacc tgcacgaggt actctcgttc gtgtccaaat tcggcacgga ggatctcatt
     6181 gacgatgtcg ccgtcgttcg tcagcaagat gagaccggac gaatccttgt ccaaccgccc
     6241 gatcggaaaa atccgctcac gatgcccgat ctcggcaatg atatttcgag ggacatgcga
     6301 ttcgctcgtt gtcgtgacac agaccggctt atgatacttg atgtagagat ttgatttgcc
     6361 ccagggaatc acacagcccg ctcgggcaat gacgtccgtt gatactacct ggtcgccgag
     6421 ttgcgccacc cggtcgttga tcgtgatgac gcccgactca attaaacggt ctgcctcgcg
     6481 tcgggaacag atcccatgat ctgtgaagaa cttattgatg cgaatggtac gcgccacatc
     6541 aagacgggag cgatggacct gcgatgccga ccgttaatgc acgcgacggc cgaccctgat
     6601 acgaccgagt atacgataga tcaaccgtgc aatggaaaat tgcggcgcca cgaatccttc
     6661 gatgggagca atctcgctga catccatgcc gacgatgccg ggcccattgg ccagcgctgt
     6721 gatgagattg agggtatcat accacccgag cccacccggc tcgggagtgc ccaaggcggg
     6781 aacgatcgac gcatccagcc catcgcagtc gaatgtcagg tacacgggcg ttgggcaggc
     6841 cgcaatcacg tcaggtatcc agttcgacgc tttgccttcg taggggccgg atggatcgag
     6901 gatggtcgcg gcaaaaaacg tcttgatgcg gaccgtggca tcgatacgag cgacctcttc
     6961 cgggctgatt gaccgaatac ccacttggac caaggacaag ccgtcttcca ccacccgcgc
     7021 catgacgctg gcatggctga acggattgcc ctgataggca tcccgtagat caccgtgggc
     7081 atcgatctgg acaacggtca tctgcggata ccgttttgca tgggcccgaa tcgcgcccaa
     7141 tgcgccagtg tgttcgcccg tcagcgtgat aagaaaccgt ccggttccta cgtggggcga
     7201 gacaaacgct tcgatggtgt cgactgccgc acgatcgaca tcccctttga gatccaatgg
     7261 tgaagccgtc gcgatcccgc cccattctcg aaacggttca cacgcgagtt gttcgtcgta
     7321 gaactcaacc tgggcggaag cttgaagaat ggccgagggg ccgcggtccg acccgcgaat
     7381 ataactggaa gtatgttcgt agggagccgg tagaacgtag acgcccgctt gatccggatg
     7441 acaccaaggt tcgtcgatcc ccaggaaatt atggtcgggc ccttcccaac cgacgggaag
     7501 cgtcatgggc gtgcttccct cggctggacc gacggctgag gcggtatgaa gacagccaag
     7561 accggcaagc ccctatttgc gagaacggtt gggcacattc tccggcggga tgaagaccgc
     7621 caatgcgatg actgtcgtcc acttaccctt tttcccgaca gccgattggg tgatgttcag
     7681 ggttctcacg atttttccac ccatcttaaa gacctgctcg cgctctttcc aggccacgtt
     7741 cggatcgaat tcgacaccta aggtcgtcgc aagcatctgc gctgcgagat cttccgtata
     7801 ctctccggtc tcctcatctg tttcaccatg ggcatgatgc tctgaaagat agccgtatgt
     7861 accgtggtcc gtgggtttcg caagcccgat cgatgccgag atgagccggt tccgttcatt
     7921 cgtttccgag cgcgccatga cgcaaaacgt gatttcacca ggtttcagca gcttctctcc
     7981 gcgcttccga gggatgatct tacaatgggg aggaaggatg gacgatacgc tgacgagatt
     8041 gcagtaggcc acaccggcgc tgcgcaacgc ctcttcgaag gaggccagct tttccttgtg
     8101 gacccccact cctctcgtta aaaacatatg cgtaggtacc atcatctccc ctttcgtcag
     8161 gtagtcggcc tgtctggttg ttcggaggtt gtttggtgac gtgttcctga gcggtcagcc
     8221 ggacaaacaa accggaccag atcaacagca gtgtcgggcg tttgttgtag caagattgga
     8281 cgggcttcgc aatatcttct agacactttt ttcaggtgag tagggtgggt ccttcagatt
     8341 catgatgagc atccgcggct ccgtcatatc ttcaatcgcg gcacacacgc cttctcgtcc
     8401 caacccggaa tctttcacac cgccgtacgg catatggtcg gcccggaatg taggaatttc
     8461 attcgccaac acggctccaa cttccaggtg acgaaacgca taaaagatct tattgatgtc
     8521 ctgcgtgaag ataccggcct gtaacccgta gtccgattga ttgagcagcg ccacggcctc
     8581 gctgatctgc cgatacggcg tgacggtcac aaccggtcca aacacctcct tacatgagac
     8641 tttcatgtcg gggttcacat tcgacagtac cgtggcttca acaagcgatc ccatccgctt
     8701 cccacccaac agcacacgcg ctccgtccgc aacggcttca ccgatccagc tctccacccg
     8761 ctgagcggct gcttgatcga tgaggggacc gatgctcgtc gcctcgtcag caggatctcc
     8821 cactttcaat cgagcgacat gcatgagcaa cttggtcgta aacgtatcgg cgatcgaatg
     8881 atgcacgaag acccgctgca ccgaaataca tgtctggccg gcatacccaa atcctcccgc
     8941 cgcgcaacgt tgggccgcaa gttcgagatc ggcatcgggc tcgatgacta ccccggcgtt
     9001 gcccccgagt tcaagcgtga cttttttctt tccacacttt gccttcagca tccatcctac
     9061 cgacgcgctt ccggtgaagc tgagcaattt gaatcgcgga tcgacgacca tccgctcggc
     9121 gaggatgtta tcgcacggca ccacattgag cccgcccgga ggaattcccg cctccatggc
     9181 cacctctcca agaaggagag ccgtcaacgg cgtctgaggc gccggcttga tcaaaatcgg
     9241 gttgccggaa gcaagcgctg gtgccacctt gtgcgccacc agattcagcg gaaaattgaa
     9301 gggggtgatg cccagaatcg gaccgatcgg gaaacgacga agtacgccaa gatgggtatc
     9361 gaagcctggc gtccaatcga gcggcaccac ctcgcccggg atccgccgag cctcttcagc
     9421 agccaccgta aaggtttgaa tcgcccgacc gacctcccgc ttcgcatcgg tgatcggttt
     9481 gcccgcctcg gccgtgatgg cttggacgaa ttcgtctcgg cgccgataga gcagagcggc
     9541 aacctgttgg aggatattgt atcgagcatg tcctggaagc tgctccatcg tcgcggctgc
     9601 actgcacgtg gaggcgatcg cctcttcaac atcggattcc gtcgcctgga tgacctgggc
     9661 gacgagtttt ccggtgaacg ggtcaacgac agaggccgag aattcgcccg acttccattg
     9721 gccatgaacg aggaacggac gggattcctg cactgggaga ctcgctggag tctgtgatga
     9781 ggttggtatt agtcagtcgc ctcaaccggc gtagtcgctt cagcggcagg ctgagcaacc
     9841 gtagctgctt cttgtgcgac gactgccttg gcgatcagtt cttccgttcc ccaatcgcgg
     9901 atcgcctcca aggtaaatcc ctcgtaggtc gacacaccat gacaagaggg gcagtccggc
     9961 atcggaactt ttctgtagaa gacttccgtg agacaagaca tacagaccca gaagtcgtca
    10021 tcgcgatggg aaacgggcca gaacgattgc atcgaagcca tgtggacacc cttatcttta
    10081 gtgacgaaga ggactatcgt acctgcgtgc gcgatgtcaa catcacgcgc aacggctcat
    10141 tctgcctcgg cgcaattcag ctattttttg tcgctcgcca gaagtcgatc gatttcttct
    10201 gcgaacatct cgaatggaaa ggctccaggg atcgcaacgg ccggctcttt ttccgtcggc
    10261 tcggcggccg ttcgcacgag aatgaaccca ggggttccat gaaaccccca ctgattggct
    10321 tcctggcggt cttcgaaaat cgcc
//
LOCUS       DATMTI010000941         6110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_628855, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000941 DATMTI010000000
VERSION     DATMTI010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628855"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VN039_14870"
     CDS             <1..226
                     /locus_tag="VN039_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47280.1"
                     /translation="SDGEVGGIHEVGGPSKRSSPPLREFLDRMVSMCEQGSHRDALQY
                     YERNRGDFMPDPALAEFDRLVETLRQKLAA"
     gene            245..>6110
                     /locus_tag="VN039_14875"
     CDS             245..>6110
                     /locus_tag="VN039_14875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV47281.1"
                     /translation="MVNPKILLVQLEFGTWNRARAWSYLGNFAVEDGLRANGCECMTI
                     PALCDIPDSSPLSWLHHAKDLLAGQRFDQVWVWLVHNRYSDAFLEWLAELAPVRVGLI
                     MESLRYGEEDCRRWPHLLERAGFVERQVKYMTHVLTADEHDADVLNRTGSVQAVFWPS
                     AVPSRFITATIERPSRRESAFYGELYGERKAWLAMPGLKDLLVHPPSAEAATEFPQLF
                     NTLHQQMSERLQSGWRPNLDALTEYVGLWRRLREAIFANWLASLKPWSAIVNLPSLFQ
                     SYAGRVVEAMAAGRPVISWKIPDRPHTLDLFKDGREILLYPRDRPAVLKEHVERITND
                     VDYAKHIATAARAELLRSHTAEARARQWLDWIETGQPLVVRHATEEAAVRDTCLAQPS
                     AQLTADRSRDEIHFATTVFLLTVDDPAFAACLEALHKQEGPSFKLDIIRNVYPFSAAA
                     QAMIDRCRSPYFIQADEDMVLAPDAVASMEAAMAAAPDDVGMICFHLFDEDRGIPIQG
                     VKIYRTALMKLLTFQDVKASEMNLLEQMGRHGIQWILHPDVKGRHGAVYAPDTIYRRY
                     KTMYEKDIRQWNVLTSDIRRKADAFRQSGDPLQLFALLGAAHGIINAPLAADREKDAR
                     TYNLAELDVFRRLFLMDPPLTQSYTVVRQVSPVHGPIPFEQVKWKGESDRNPAPTVAD
                     CAVTRENGAGTKSVLIVTPHFWPSVGGVEHVAEDLGVGLLDHGYRVDIATYPHAERHL
                     TMHRGLTIITLSPHDRQEGAIPICALEVERLIGSGRYDVCVMLGAPVNGLFYGAMTGL
                     LPDKTRLIFQPTLNKEICELLSASTSLATDLVVRLTARAHHVVVLSEQGCDASFFGRH
                     GLETTYLPNGTQARTSKGEFRKDHGIPSDAFIILHVANLHPVKNHVGLLEAFASMPQG
                     AKLVLVGHPTDNTAYVVQVRQALAGRPEVLYIPGLSPEGVAAAMQAADVLVLPSQAEA
                     SPLCILEAMSHRLPWVATPECDAAHEQAGGVVIPLVDFRRAVIILMQEPSLRRALGET
                     GYAHWEACRQWSSVLAGWIELIETGRLPRSYTMPPDLVERTIAQQRAFSKQLEQVEPE
                     FLEVTTAGSGLSTSNVTKQTNGGAMNSDAFYVNLFVNAPAWSAPHPNTDEAARWSKIA
                     AFLEYILREVQQKGPDRRLRILDVGCGRGWLTNLATMYGTCEGVEPVAGVVEHARKLF
                     PHLRFEVGTAEIVSGRADFEPYDVILTSEVIEHVPHGRKENFLAQLFRLLKPDGYVVL
                     TTPRGEMWEQWKTITPPNQPVEDWVTEEQLRALFTSQGFVEMGLDRIHVEVPGLRYVP
                     APTPADLRSMNLLPIYQVWCCRRSVDQRPIPFMRTPKVSIIVPTYNRPDRLRTALSSL
                     AAQTYQDFEVIVVNDAGCDVGFVVAACADRHRIMTITHDRNRGLAAARNSGLRAAKGT
                     YIAYLDDDDRYLPNHLETLVEYLDRRECRVAYTDAWRVQERQSDSGYVETGRDVPYSY
                     DFRPTDLLVSNYFPVLCVMHDRACLDEVGLFDESLFAHEDWDLWIRMATRFPFKHLPV
                     RTAEFTWRTDGSSMTSGTRETYWRTTEIIYRKYRPYADLICGVREAQDKRLTEMQAVG
                     SAKHYVCSIVMPVWNRVELTKDCLTALAGLKDQPEYELIVVDNGSTDGTTDFLRQLSG
                     DVRVIVNEENLGFAKACNQGAAAARGKYLVFLNNDTIPQPGWLTALVAEVDNYVEVGV
                     VGSKLLYPDGTVQHAGVVRDCARRLPYHIYKGFAASHPAVNQRREFQIVTAACLLIRR
                     AVFEEVGGFDEGYVNGFEDADLCLKVRERGYVVVYQPRSVVVHLESQTPGRKLHDDVN
                     ATRFLDRWRAQWWAGDEDLHFHVDGYKLKRVFRNGRDGGDILMFSDIKDRAAWAHVAA
                     TQAAALKQDWQAVRRELSLVSDWPDDPEILAWASSVCEQLQELKLRQTFLSRYLA"
BASE COUNT         1247 a         1701 c         1857 g         1305 t
ORIGIN      
        1 gagtgatgga gaggtgggcg gtatccacga ggtgggtggt ccgagtaagc gatcatcgcc
       61 gccgctccgc gaatttttgg accgaatggt ttcgatgtgt gaacaaggat cgcatcgtga
      121 tgcgttgcag tactacgaac gcaatcgcgg cgatttcatg ccggatcctg cgttagcgga
      181 gttcgaccgg ctggtggaaa cgcttcgcca gaagctggcg gcgtagtcgc ggcacgaggc
      241 gttcatggtg aaccccaaaa tcctcttggt gcaattggaa ttcgggacgt ggaaccgagc
      301 aagagcctgg tcctacttgg gaaacttcgc ggttgaggac ggcctccgtg ccaacgggtg
      361 tgaatgcatg acgattccgg cgctctgcga cattccggat agctcgccat tgtcctggct
      421 gcatcatgcg aaagacctgc tggccggtca gcggtttgat caagtctggg tatggctggt
      481 gcacaatcgc tattcggatg catttcttga atggcttgcc gaactggcgc cggtgcgggt
      541 cgggctcatc atggagtcgt tgcggtatgg cgaagaggat tgccgacgtt ggccgcactt
      601 gctagagcgg gctgggtttg tcgaacggca agtgaagtac atgacgcatg tgctgaccgc
      661 agacgaacac gatgccgacg tgctcaatcg gacaggttcg gttcaggctg tattttggcc
      721 gtcggcggtg ccgtcccgtt tcattaccgc aacgatcgag cgaccatccc gccgtgaatc
      781 agcgttttac ggggaactgt atggagagcg gaaggcgtgg ttggccatgc ctggcttgaa
      841 ggacctgctg gtccatccac cgtccgcaga agcggccaca gagtttcctc agctcttcaa
      901 tacattgcat cagcagatgt ctgaacggct ccagtcgggg tggcggccga atctagacgc
      961 cctcacggag tatgtgggcc tgtggcgtcg gttgcgcgaa gccatttttg ccaattggct
     1021 ggcgagcctc aagccttgga gcgcgatcgt caatctcccc agcttatttc aatcctatgc
     1081 aggacgtgtc gtggaagcca tggccgcggg tcgtcctgtc atctcatgga agattcctga
     1141 tcggcctcac accctggacc tgtttaagga tgggcgggaa attctcctat atcctcggga
     1201 tcgtcccgca gtcttgaagg aacatgtgga acggatcacg aatgatgttg attacgccaa
     1261 gcacatcgct acagccgctc gcgcggaact gctccgatcg cacacggccg aggctcgtgc
     1321 acggcagtgg ttggactgga ttgagaccgg gcagcctctc gtggttcgtc atgcaacaga
     1381 ggaggcagct gttcgggata cgtgcttggc tcagccgtcg gcccagctca ctgccgaccg
     1441 atcccgcgac gagatacatt tcgcgaccac ggtcttcctg ctgacggtag acgatcctgc
     1501 ctttgctgct tgcctggaag ccctgcacaa acaagaaggg ccgtcattca agctggacat
     1561 tatccgcaac gtttacccgt tcagcgcagc ggcgcaagcg atgatcgatc gctgtcgctc
     1621 accctatttc attcaagccg acgaagacat ggttttggcg ccggatgccg tggcctcgat
     1681 ggaggcggcg atggccgccg cgcctgacga cgtcggaatg atctgctttc atttgttcga
     1741 cgaggatcgc ggcattccga ttcaaggcgt gaagatttac cgcacggcgc tcatgaagtt
     1801 gctgacgttc caagacgtca aggccagcga gatgaacctc ttggagcaga tgggccgtca
     1861 cggcattcaa tggatcctcc atccggacgt caaagggcgg cacggggcgg tctatgcacc
     1921 cgacacgatc taccgccgct acaagaccat gtatgagaag gacattcggc aatggaacgt
     1981 tctaacgtcc gacatccgcc ggaaggccga tgcgttccgg caatccggcg atcctctgca
     2041 gctcttcgca ttgttgggtg cggcgcacgg tatcatcaac gcccccctgg cggccgatcg
     2101 cgagaaggac gcccgcacct ataatcttgc ggaactcgat gtattcaggc ggctgtttct
     2161 gatggatccc cccctgacac aatcctatac cgtcgtgcga caagtatcgc ccgttcacgg
     2221 accaataccc tttgagcagg tgaagtggaa gggggagagc gatcgtaatc cggcgcccac
     2281 agttgcggac tgtgccgtga cgcgggagaa cggagcggga accaaatcag tactgattgt
     2341 gaccccgcat ttttggccat cggtcggagg agtggagcat gtcgcagaag accttggagt
     2401 cggattgttg gaccatggat accgcgtcga cattgccacc tatccgcatg ccgagcgtca
     2461 cctaacgatg cacaggggac ttacgatcat cacgctgtct ccgcacgatc gacaggaagg
     2521 ggcaatcccg atctgtgcgc tggaagtgga gcggctgatc ggctccggtc ggtatgacgt
     2581 ctgtgtgatg ttgggcgcac cggtcaatgg cctgttctat ggagccatga ccggtttgtt
     2641 gccggacaag accagactga tctttcagcc gacgttgaac aaagaaatct gtgaactgtt
     2701 gtcggccagc acgagtctcg cgacagacct ggtggttcgg ctcacggctc gtgcgcatca
     2761 cgtggtggtg ttgtcggagc aaggctgcga tgcctccttc tttggccgac atggtctcga
     2821 gacgacctat ctgccaaacg gcacccaggc ccgaacgtcc aagggagaat ttcgcaagga
     2881 ccatggaatt ccatccgatg cattcatcat cctccatgtt gcgaatctcc acccggtgaa
     2941 aaatcatgtg gggctgcttg aagcgtttgc ttctatgccg caaggcgcga aactcgtctt
     3001 ggtcggccac ccgaccgaca acaccgcgta tgtcgtgcaa gtacggcagg cgctggccgg
     3061 ccggccggag gtgctttata ttcccggtct ttcgcccgaa ggcgtcgcag cggccatgca
     3121 ggcggcggac gtcctggtat tgccctcgca ggctgaagcc tctcctttgt gcattcttga
     3181 agcgatgagt catcgattgc cgtgggtggc gacgcccgaa tgcgacgctg cgcatgaaca
     3241 agccggcggc gtggtgattc ccttggtcga tttccgacgg gccgtaatca tcctgatgca
     3301 ggagccttca ttgcgccgag cactcggcga aacgggctat gcgcattggg aagcctgtcg
     3361 ccaatggtct tcagtgctgg ctggctggat cgaactgatt gaaacgggac gcctgcccag
     3421 gtcctatacg atgccgcccg acttggttga acgtacgatc gcgcaacagc gggctttctc
     3481 gaaacaattg gaacaagttg agcccgaatt cctcgaagtg acgaccgcag gaagcggact
     3541 gtcgaccagc aacgtgacga aacagaccaa cggaggagcc atgaactcgg acgcgtttta
     3601 tgtaaacttg ttcgtcaatg ctcctgcttg gtcggcgccg catccgaaca ccgatgaggc
     3661 ggcgcggtgg agcaagatcg cggcctttct ggaatacatt cttcgcgaag tccagcagaa
     3721 ggggcctgat cggcggctgc gcatcctgga cgtcggatgt gggagaggct ggttgacgaa
     3781 tctggcgact atgtatggga cctgcgaagg ggtcgagccg gttgccggcg tcgtcgagca
     3841 tgcgcgcaag ctgtttcccc atcttcggtt tgaagtgggg acggcggaga tcgtatcggg
     3901 tcgcgccgat ttcgaaccct atgatgtgat tctgacatcg gaagtcatcg agcacgtgcc
     3961 tcatggccgg aaagaaaact tcctcgcgca actattcaga ctgttgaagc cggacgggta
     4021 tgtcgtgctc acgaccccgc gcggtgaaat gtgggaacag tggaagacca tcacgccgcc
     4081 caatcaaccg gtggaagact gggtgaccga agagcagctg cgggccctgt tcacctcgca
     4141 gggcttcgtc gaaatgggat tggaccgtat tcatgtcgag gttccgggct tgcgttatgt
     4201 gccggcgccc accccagccg acctccgctc catgaatctg ctccccatct atcaagtgtg
     4261 gtgctgccgc agatccgtcg accaacgtcc cattcccttt atgcgcacac cgaaagtgtc
     4321 catcatcgtc ccgacataca atcgccccga tcgtctcagg acggcgctct cgagtctggc
     4381 cgcgcagacg tatcaagact tcgaagtcat cgtcgtcaac gatgcgggtt gtgacgtggg
     4441 gttcgtcgtc gctgcctgtg ccgatcggca ccgcatcatg accatcaccc acgatcgcaa
     4501 ccgtggattg gctgcagccc gcaacagcgg actccgtgcc gcaaagggta cttatatcgc
     4561 ctatctcgat gacgatgatc ggtatcttcc gaatcacctg gaaacgctcg tcgagtattt
     4621 ggatcgccgt gaatgccgcg tcgcgtacac cgacgcttgg cgagtgcagg aacggcagag
     4681 cgacagcggt tatgtcgaga ctggtcgaga tgtaccgtac tcctatgact tccggccgac
     4741 ggatctactg gtgtccaact attttccggt tctttgcgtg atgcacgatc gagcctgtct
     4801 cgatgaggtg ggcctattcg acgaatctct cttcgcccat gaagattggg atctctggat
     4861 ccgcatggcg actcggttcc cctttaagca cctgccggtc aggacggccg agttcacctg
     4921 gcgaaccgac ggaagttcca tgaccagcgg gacgcgtgag acctattggc gcacgacgga
     4981 gattatctac cggaaatacc gaccctatgc cgacctcatc tgcggtgtgc gtgaagcgca
     5041 ggacaaacgt ctcaccgaga tgcaggcagt cggaagcgcc aagcattatg tctgttccat
     5101 cgtcatgccg gtttggaacc gagtggagct cacaaaagat tgtctgacgg ccttggccgg
     5161 attgaaagac cagccggagt acgaactgat tgtcgtggac aatgggtcca ccgacggcac
     5221 aactgatttt ctccgtcaac tgagcggtga tgttcgggtc atcgtcaatg aggagaatct
     5281 cggattcgcc aaggcttgca atcaaggagc cgcagctgcc cgagggaagt atctcgtctt
     5341 tctgaacaac gacacaattc cccagccggg atggctgacg gcgctggtgg cggaagtaga
     5401 caactatgtg gaggtggggg tggtcggcag caagctcttg tatcctgatg ggacggttca
     5461 acatgccggc gtcgttcggg actgtgcgcg ccgcttgccc taccatatct acaagggctt
     5521 tgccgccagc catccggccg tgaaccaacg ccgagagttt caaatcgtca cggccgcctg
     5581 cctgctgatc aggcgggccg tgtttgagga agtgggggga ttcgacgaag gatatgtgaa
     5641 cggattcgaa gatgcggatc tgtgtctgaa agtacgggag cgcggctacg tcgtcgtgta
     5701 tcaaccccga agcgtcgtcg ttcatctcga aagccaaact cccggaagga agctccacga
     5761 tgatgtgaac gcaactcgtt tcttggaccg gtggagggcg caatggtggg cgggcgacga
     5821 agacctgcat tttcatgtag acggttacaa attaaaacgc gtgtttcgca acggccgcga
     5881 tgggggtgac atcctgatgt tcagcgacat caaggatcgt gcagcctggg cccacgtcgc
     5941 cgccacccag gccgccgcct tgaaacaaga ctggcaagct gttcggcgtg agctcagcct
     6001 ggtttcggac tggccggacg atcctgagat attggcctgg gcgtcatctg tctgcgaaca
     6061 gttacaagaa ttgaaactgc gacaaacttt cctttcccgg tatcttgccc
//
LOCUS       DATMTI010000942         1612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_586234, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000942 DATMTI010000000
VERSION     DATMTI010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586234"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..419)
                     /locus_tag="VN039_14880"
     CDS             complement(69..419)
                     /locus_tag="VN039_14880"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWV47282.1"
                     /translation="MSSVLRDNRDVLLYRCHFEPRRTLDYIDAKSAHLLDDSSDGFPS
                     GQGQALGDWTHPLDREPAWNAIEEAVRSQRSYLLVYRLIGTEGKDKWVWDEGEAFKTD
                     DGRIAGLEGFLAGI"
     gene            complement(544..966)
                     /locus_tag="VN039_14885"
     CDS             complement(544..966)
                     /locus_tag="VN039_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV47283.1"
                     /translation="MTHEVATATDSITAKEAIDVVRSRNVPILVVYRRSEPIVALTEY
                     DLAINLITEHRADSVTLYELIKKRMVIRCREDAILADAMDAMLNHCIRHVPVVNRSGG
                     LVGALSLVDAVGAITPDAAALWKTKVRRVWTETPEFKR"
     gene            complement(1191..>1612)
                     /locus_tag="VN039_14890"
     CDS             complement(1191..>1612)
                     /locus_tag="VN039_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47284.1"
                     /translation="LKREEARMKSIQKIIGIVLCGVVLSVGASSIGLAEHSPSPGDVM
                     KTDSLTDAQKYNFQSDDDKQRNVNDGKSHASGAKTIKGELFRVDDANYFVKTKDGAEV
                     RLHTDRTTNMRGKIRKGDQIEATINNQNHALSIQAIR"
BASE COUNT          378 a          432 c          388 g          414 t
ORIGIN      
        1 ataagggtcg acggcgggac tcgtactggg cggaccccta gataggactt agggcagaaa
       61 aacgtgtatc aaataccggc gagaaatcct tccaagccgg cgatgcgtcc atcgtcggtt
      121 ttgaacgctt cgccttcgtc ccatacccat ttgtctttgc cttccgtacc gatgagccga
      181 tacaccagca ggtaagaacg ttgggagcgg accgcctcct caatggcatt ccatgccggc
      241 tcacgatcga gcggatgggt ccagtcaccg agcgcttgcc cctgccccga cggaaatccg
      301 tcggatgaat catcgagcag atgggcgctt ttggcatcga tgtagtccaa tgtccgacgc
      361 ggttcaaagt gacatctgta caacaggaca tctctgttat ccctcaatac tgaactcaca
      421 ggtcgactct tcgagctgcg cctttttacc attgaggggg acctggccat agcttgacat
      481 tatcggaaca tatcaagaaa tgaactggga aataccagag cgaggacaca tcggaggggt
      541 gctctatcgc ttgaactcag gcgtctcagt ccatactcgt cgtacctttg ttttccaaag
      601 tgctgcggca tccggcgtaa tggctcctac cgcatccacc aacgaaagcg caccgactaa
      661 acctccgctt ctattcacca ccggaacatg acggatgcaa tggttgagca tggcgtccat
      721 tgcatcggct aaaatggcat cttcgcgaca tcgaatgacc attcgcttct tgatcagctc
      781 atagagagtg accgaatcag cacggtgttc ggtgatgaga ttgatggcca agtcatattc
      841 cgtgagcgcg acgatcggct cgctccgtcg gtacacaacg aggatgggga cgttccggct
      901 tctaacgacg tcaatcgctt ctttggctgt gatggagtct gtggcggttg ctacctcatg
      961 agtcataacg tcttttacaa ggcggtcttc accaggaagc acactgtatg cttcatcatt
     1021 ggtggattcc ccaaaaattc cagaacggcg gcgggggatc acagtaagca ttggcatgct
     1081 tttaacggaa tatccggctg aattgtactg ggcgaatccc taatataacg aaacatagct
     1141 ctgccacctc cagagctgca cctgccgatc atgatcacat tgtgaagatg ttatcggatc
     1201 gcttgaatcg acagcgcatg attctgattg ttgattgtcg cttctatctg gtcacccttt
     1261 ctaatttttc ctctcatgtt ggtggtccta tcagtgtgca aacgaacttc ggctccatcc
     1321 ttggtcttga caaaataatt ggcatcatca acgcggaaca attcgccttt gatcgtcttc
     1381 gcaccgcttg catgggactt cccgtcgttc acattccgtt gcttatcatc atcagattga
     1441 aaattatact tttgcgcatc ggttaaagaa tctgttttca tcacatctcc gggactaggg
     1501 gaatgttcgg ccagccctat actggacgcc cctacggaca acacgacacc gcacaataca
     1561 atcccgatga ttttttgaat tgatttcatg cgagcctcct cacgttttag tt
//
LOCUS       DATMTI010000943         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_502523, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000943 DATMTI010000000
VERSION     DATMTI010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502523"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1429
                     /locus_tag="VN039_14895"
     CDS             194..1429
                     /locus_tag="VN039_14895"
                     /inference="COORDINATES: protein motif:HMM:NF023301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HWV47285.1"
                     /translation="MIRITSLFLASLLLLAGCESPDRVLTNADRVLGSRTGRTVLDIA
                     TGKDPKLILKERVDAYQRDPEAVIRDLRTIQRDFNTIMTALTGRVRQTWGEKEVKVPE
                     QKKYVKYTQNYLSRTIVDFDNGSIVVETLDDKSPKESLKNAIVTTLLTPDDPRSVDLF
                     SDKPVTLTSDHPPYLLGLVLDEQGQPIRTPAQAEAFAASTLESAKTRTVDQDGATKQA
                     LIAEIKMVANFSNRQANKYRSTVTRFAEQFKISPSLIFAVIRTESNFNPFAVSFAPAY
                     GLMQLVPTSGGRDAYRRAKGKDTIPSRDYLFDPENNIELGSAYLNVLSYNLLEQIDNE
                     VSREYCVISAYNTGPRNVFKTFAGDSVAALNRINSLEPPAVYDRLRNNLPYQETRDYL
                     MKVVTFRKQFVSLRENNDR"
     gene            complement(1472..1873)
                     /locus_tag="VN039_14900"
     CDS             complement(1472..1873)
                     /locus_tag="VN039_14900"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV47286.1"
                     /translation="MMDDSDFRAGSGQITPQLRKHPRVRVSAPFPCSLARVGLLRKGA
                     VEQGLGIIYDVSAKGARVMTEAVITPGDRIAMRLRLPDQAALMVIETATVRWGKEQTY
                     GVEFEELSPNDNKHLQAFMNHQSRSGQSLAV"
     gene            complement(1877..>2198)
                     /locus_tag="VN039_14905"
     CDS             complement(1877..>2198)
                     /locus_tag="VN039_14905"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV47287.1"
                     /translation="HISTPFSCSLSLLGTRWWFRKPVSDVGMVYDLSLHGVCVSTDAD
                     IKPGDQVSLALRLTKGTPPAEVAVATVCWTNHQFHGLAFSTLSQTSLRQLTEYMDASS
                     IAKE"
BASE COUNT          545 a          632 c          576 g          445 t
ORIGIN      
        1 gaagaaggga tgacgacgct tgtgcaagac ggcattcaaa aagtcttgca gggccacacg
       61 acatacaaag aagtcaaagc cgtcgccatc aaatagtgct ttcgatttac ccggtacgac
      121 cggctttctg gagacagtcc ccttcattcc cacccacaac cgtgtagaat gcgccgccca
      181 gcatggagca ctgatgatcc ggatcacatc gcttttcttg gcctcgctgc tccttctcgc
      241 gggatgtgag tccccggacc gcgtactgac aaacgctgat cgagtgctcg gcagccgaac
      301 cggtaggacc gtgctcgaca ttgccaccgg caaagacccc aaactaatcc tcaaagagcg
      361 cgtcgatgcc tatcagcgcg accccgaggc cgtgatcaga gatctgcgaa cgatccaacg
      421 ggatttcaac accatcatga ccgcgctgac aggccgggtc cgacagacgt ggggcgagaa
      481 ggaagtgaag gtcccggagc aaaagaagta cgtgaaatac acgcagaatt atttgagccg
      541 gaccatcgtc gacttcgaca acgggtcgat cgtggtggaa acattggatg acaaatcgcc
      601 gaaggagagc cttaaaaacg cgatcgtcac gaccttgctc acaccggacg atccgcgctc
      661 cgtcgatctt ttttccgaca agccggtcac actgaccagt gaccaccctc cgtatctatt
      721 gggtctggtg ctggatgaac aaggtcagcc gatccgcacc ccggctcaag cggaagcgtt
      781 tgcggcatca acactcgaga gcgcaaagac aagaaccgtg gaccaagacg gggcgaccaa
      841 gcaagcgctg atcgccgaaa tcaagatggt cgcgaatttc tcgaaccggc aagcgaacaa
      901 gtatcgctcg actgtgactc ggttcgcaga gcagttcaag atcagtccca gcctcatctt
      961 tgccgtcatt aggacggaaa gcaacttcaa cccctttgcc gtgagtttcg ccccggccta
     1021 tggcttgatg cagctggttc ccaccagtgg agggcgggat gcctatcgaa gagccaaggg
     1081 aaaagatacg attccatcac gcgattacct gttcgacccg gagaacaaca tcgagctcgg
     1141 cagtgcctac ctcaatgtcc tgtcgtataa tctgctcgaa cagatcgaca atgaggtttc
     1201 gcgggagtat tgcgtgattt cggcctataa cacaggccct cggaatgtct tcaagacatt
     1261 tgcaggcgac tccgtagccg ctctcaatcg catcaatagc cttgaaccac cggccgtcta
     1321 tgaccgattg cgcaataatc tgccgtacca ggaaacaagg gactacctca tgaaggtcgt
     1381 caccttccgc aaacagttcg tctccctacg agaaaacaat gaccgctaag agccttcagg
     1441 gtgttcagaa acactaggca aggctggaaa gtcagacggc tagagactga ccggatcggg
     1501 actggtggtt catgaaggct tgcagatgct tattgtcgtt gggagacagt tcttcgaact
     1561 ctactccgta ggtttgctct ttaccccacc gaaccgtggc ggtttcgatg accattaagg
     1621 ccgcctgatc gggcaatcgc aaacgcatgg cgattcggtc tcctggtgtg atcaccgctt
     1681 ccgtcatgac acgcgcccct ttggcagaca cgtcatagat gatccccaga ccttgctcga
     1741 ccgccccttt cctcagcaaa ccgaccctgg ccaatgaaca gggaaatgga gccgacacac
     1801 gcactcgagg gtgtttgcgc aattgagggg taatctgtcc actgccggcc ctgaagtctg
     1861 aatcatccat catgttttac tctttcgcta tacttgaagc atccatgtac tcagtcaact
     1921 gtctcaacga cgtttgcgat agcgtgctga acgcaagtcc atggaactga tggttcgtcc
     1981 agcaaacggt ggccaccgcc acctccgctg gaggagtgcc cttggtcagt cggagtgcga
     2041 gggaaacctg atcacccggc ttgatgtccg cgtcggtgct cacgcataca ccgtgcagag
     2101 acaggtcata gaccatacca acatcactga ccggttttcg aaaccaccat ctagttccca
     2161 gcaaggaaag ggaacagctg aagggcgtgg agatgtga
//
LOCUS       DATMTI010000944         9088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_629018, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000944 DATMTI010000000
VERSION     DATMTI010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629018"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..171
                     /locus_tag="VN039_14910"
     CDS             4..171
                     /locus_tag="VN039_14910"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47288.1"
                     /translation="MALGDCAINCGMFKGSYAVTGPVERHIPVDVRIPGCPPRPADIL
                     EALSELRKGRP"
     gene            complement(273..2585)
                     /locus_tag="VN039_14915"
     CDS             complement(273..2585)
                     /locus_tag="VN039_14915"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV47289.1"
                     /translation="MTQPEERTASTARRYEWLTFVMVLITLFTLGVGTFLLGHVERSI
                     VAAVWLPLLMLLLWSTIRLRAEYRQAQQESAWARAAEAALLQSQERNRAIVDTALDGV
                     ITIDAAGIVTEWNAQAAAIFGWTREEAMGKLLSDTIIPERDREAHAQGIREYLKTGVG
                     PVLNRRIEIAARHKEGHEFPVELAVSPARIGEAYIFSAFVRDITDRRRAERRLASQYA
                     VTRVLSEAVRLEEAVPKIIQAVGESLEWDLGIFWRLDKQSGTLRCLDHWRVPTLSVNE
                     FIAQIQHQTFKPGVGLPGRIWESGRPVWIKDVTLDPIFVRADLAAQAGLHGAFGFPIR
                     IGGEVEGVIEFFSHQVREPDSELLSMIADVGLKIGQFGERTRAEEALRLAEAQLRQAQ
                     KMEAVGRLAGGVAHDFNNLLTVIRGYSELVLSRLGSGDPARREMEEVKKSADRAAGLT
                     SQLLAFSRRQFVATKIVDLNAIVMNMDGMLRRLLGEDIIELCADLEPQLGSIKADPGQ
                     IEQVIMNLAVNARDAMPTGGQLTIQTRNVTIGKGPRRETMMLNEGTYVLLAIRDTGHG
                     MSEETQSHLFEPFFTTKEKGKGTGLGLSTVYGIVKQSGGTIGIESKQGQGTICKIFFP
                     KVDEITQAVPVVNGVVGRAIGRETILVVEDDPSVRGLVQEALRLNGYEVLVARHGIEA
                     LLTGAKHPGPIHLLLTDVAMPQMSGPEVAEKLTVVRPEIKVLYMSGYPDHPVFEQGGI
                     KRDTAFLQKPFTPNGLTQKVREVLDGKKVA"
     gene            complement(2590..4131)
                     /locus_tag="VN039_14920"
     CDS             complement(2590..4131)
                     /locus_tag="VN039_14920"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HWV47290.1"
                     /translation="MIGSEPSLLQLGMHRMIGISMDLMTVDSKQTLRVLPQLVPDSAC
                     FRCDVCCRFPEADSFLRPYFTRSEISDAVEHGIPESFFPDKFGSQVGLVKNPIGEGYL
                     CPAFDSKSGRCGIYDVRPLDCRLYPMALMWDLSGREVLLGWDSKCPFMRDAPSSAIRE
                     YAERVAIFLAGEAIVETIAAHPRFIGSFQDDVVVLKTLPHLTARLGPQRVDFRLHPLT
                     PSDGSYFEKALERAEVLCPETPAAFAFPYHYIWSSLLPYWWMEFDRTLFLFVQSPDGW
                     FMPLPPLGAGPLDRAVEQAFALMRSWNGTSPVSRIENVMESQRQLLECDGIRFRRKEG
                     DYLYPVEALAALAGDHYKSQRALCNRAAREQAITKEPYRADDHSGCARLYRRWAEQKH
                     RGNLDQVGKLLLEDAEMAHALVFQEHERIGLSGTVLRVNHDIVAYTFGYWLTPQTWCI
                     LLEVADRSIPGLAQWLFRDTCRTAMAQGAVFINAMDDAGLPGLRAAKSAYRPSAILDT
                     WVITR"
     gene            4263..5693
                     /locus_tag="VN039_14925"
     CDS             4263..5693
                     /locus_tag="VN039_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47291.1"
                     /translation="MTQPESSCPTLSSEAARDRCIAIRTRLQDASLFGVTAAWRVSPC
                     PFYLSSDQLQFFINLGPQLLSFYRALNRLYNESIKGIQPVWISTYLDQGKPDALVQYS
                     RMKRFREALPTVIRPDVIPTQDGMVITELDSVPGGIGLTACLSYSYHDLAGGDSQLIG
                     GRDGMVRGFARMLRSVQGQREGCIAILVSEESKDYRPEMSWLAAQLREAGMPVWCVAP
                     HEIRFTEEGLRLHIDGSERAIGVVYRFYELFDLLNIPKAELVQYAAKKGRLSVTPPYK
                     PTLEEKSAFALLHHPRLRPFWEKELGADGMLHVSAIMPKTWLLDPAPLPAIATIPGLR
                     IGGQAVTNWSELETATQKERHFVIKPSGFSALAWGSRGVSIGHDMPQSGWVEALRNAL
                     ASFPATPYILQEFHKGRLFEMDYLDEGMQTIVRMSGRARLSPYYVVSDDKAELTGILA
                     TICPADKKILHGMKDAIMVPCALRSN"
     gene            5771..6103
                     /locus_tag="VN039_14930"
     CDS             5771..6103
                     /locus_tag="VN039_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWV47292.1"
                     /translation="MTLFHVDWCPDCHVVRQKLADLGVTYESVVVPDSRRMRTQVYEV
                     SGQYYVPVLKDDDLVLTETDDILAYLDERYSADRAEADTATEFQSQARTTPEVSGKDD
                     EHPSCRIN"
     gene            6121..7314
                     /locus_tag="VN039_14935"
     CDS             6121..7314
                     /locus_tag="VN039_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HWV47293.1"
                     /translation="MEDWRQVLARSIVKPRDLAERFGLDEKEIEAIVGPYPMRITPTV
                     LETIKSKDDAIWKQVVPDPAELNDIAADDDPLEEDLMSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKQGELDRAIQYLREHNEVRDVILSGGDPLLLPDHLLER
                     ILKALRTIPHLELIRIGSRVPGSLPQRITSKLCEMVKKYHPIYMNLHFNHPDELTPEV
                     KAACGMLADAGIPLGAQTVLLKGVNDDPEIMKRLVHQLLLARVKPYYLYQADLTKGTN
                     HFRTTVETGLNIIKALQGHTSGMAVPHFVIDAPGGGGKIPLLPDNYVVHLDEDGAILR
                     NYEDKTFHYPQPKSDNRRELPMIGVQSPLDDPEEAYAACSDSCPDRDGYAAWGNSCGG
                     DADDL"
     gene            7311..8468
                     /locus_tag="VN039_14940"
     CDS             7311..8468
                     /locus_tag="VN039_14940"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HWV47294.1"
                     /translation="MTTHSPRAGILPYQDIKRLIASRAISASPTVEERQIQPASLDLR
                     LGRKAYRLISSFLPELSAISSRLDVLDFYQSDLVMYEMDLTDGAILEKGHVYLVPLLE
                     QLALPKTIRARANPKSTTGRLDVFTRVVTDLTAGFDEIRAGYRGQLFLEVVPRSFAIK
                     VRTGQSLNQIRFVRGEATVPDQALQALHRHTPLLYHNTASHKTVDNRDFRVERGLFLR
                     IDLAGTDQRDTRVIGYRAKKNSHVIDLEKVGHYAAADFWEPLHRHRHDSLLLEPEEFY
                     ILASKERIRVPAGYAAEMVAYEAACGELRTHYAGFFDPGFGYGSKGEIKGTQVVLEVR
                     PHDVPFLIHDGQTFFKVVYDKMIGMPAQLYGSPLGSSYQRQTLTLSKHFKV"
     gene            8616..9029
                     /locus_tag="VN039_14945"
     CDS             8616..9029
                     /locus_tag="VN039_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47295.1"
                     /translation="MAPSDLPPQGEPPRSSRPAKAGEQGGGGNEHHIHVIGLMVGSAL
                     MFIGFLNVFLSISGGFEINVMPFLVYFGGLAIWANAAIETPALRYGTMAIAISLALGL
                     FQYGEVLFWHKQVLFWVTIIVVMYFMFVEPKKPTP"
BASE COUNT         1972 a         2746 c         2458 g         1912 t
ORIGIN      
        1 gtcattgcgc tcggtgactg cgcgatcaac tgcggcatgt tcaaaggcag ctatgcggtg
       61 accgggccgg tcgaacgaca cattccggtc gacgtccgta tcccgggctg tccgccacgc
      121 ccagctgaca ttctcgaagc cctgtcggaa ttgcgcaaag gaaggccatg aggcgcaacg
      181 gaaggatgcc tatcacggat ccgatgacgt atggatgaaa aaggccgggc aggtgcagcg
      241 cacgagaacg ttaagacagg gaccgcagtc tgctaggcca ccttctttcc atcgagcact
      301 tcacgaactt tttgggtcaa tccattgggc gtgaagggct tctggagaaa agccgtgtcc
      361 cgtttgatcc caccttgttc gaacaccgga tgatcggggt agccggacat gtacaagacc
      421 ttgatctcgg gtcgcaccac cgtcagtttt tctgcgacct ccggcccgct catctgcggc
      481 atcgcgacgt ccgtcaacag caaatggatc ggacccgggt gcttcgcgcc ggtcaagagc
      541 gcttcgatgc cgtgacgcgc gaccaacacc tcgtacccat tgaggcgaag ggcttcttgc
      601 acgagcccgc gcaccgacgg gtcgtcctcg accaccagta tcgtttcacg cccgatcgcc
      661 ctgcccacca ctccattgac gaccggcacg gcttgtgtga tctcatccac tttcgggaaa
      721 aagatcttgc agatggttcc ctgtccttgc ttgctctcga tcccaatcgt tcctccgctt
      781 tgcttcacga tgccgtacac agtggacaac ccgaggcccg tccccttccc tttttccttt
      841 gtggtgaaaa acggctcgaa caaatgcgat tgggtttctt cgctcatgcc atgacccgtg
      901 tccctgattg ccagaagcac gtacgttccc tcgttgagca tcatggtttc acgtcgaggg
      961 cctttcccga tggtgacatt ccgagtctga atcgtcagct ggccacccgt cggcatggca
     1021 tcccgagcat tcacggccag attcatgatc acctgctcga tctgccccgg atcagccttg
     1081 atcgatccca actgcggctc gaggtcggca cagagctcaa tgatgtcctc acctaacagc
     1141 cgccgcagca tcccatccat gttcatgacg atcgcgttca gatcgacgat cttggtcgcc
     1201 acaaattgcc gccggctgaa tgccagcagt tggctggtca ggccggcggc ccgatcagcg
     1261 gattttttga cctcttccat ttcgcgtcgc gcgggatccc ccgaccccaa gcgactcagg
     1321 accaactcgc tgtatccacg aatcaccgtc agcagattgt tgaaatcatg ggcgacacca
     1381 ccggcgagcc tccccacggc ctccattttc tgtgcttggc gcagttgcgc ctccgccagg
     1441 cgcaacgcct cttcggctct cgtgcgttca ccgaattgcc cgatcttcag gccgacatcg
     1501 gcgatcatac tgagcagctc gctatccggc tcacggacct gatggctgaa gaactcgatg
     1561 acgccctcga cttcgccacc gatccgaatt ggaaacccga acgcgccatg aagcccggct
     1621 tgagcggcca aatccgctcg aacaaaaatg ggatcgagtg tcacgtcttt gatccacacc
     1681 ggccgcccgc tttcccagat tctgcccggc aaccccacac cgggcttgaa ggtctgatgc
     1741 tgaatttgcg cgataaactc attgacggaa agcgtgggga ctcgccagtg atcgaggcac
     1801 cgcaacgttc ccgattgttt atccagtctc caaaatatac cgagatccca ttcaagactc
     1861 tccccgaccg cttgaatgat tttcggcacc gcttcttcga gcctcacggc ttccgagagg
     1921 acacgcgtca ccgcatattg ggacgccagc cgtcgttcgg cccgacgacg atccgtaatg
     1981 tcccgcacaa acgcgctgaa gatataggct tcaccgatac gcgccggcga gacggcgagc
     2041 tcgaccggga actcgtggcc ttccttatgt cgtgcagcga tctcgatccg acgattcagc
     2101 accgggccga cgccggtctt gagatactct cgaatccctt gagcatgcgc ctcccgatcc
     2161 cgctcgggga tgatggtgtc cgaaagcaat ttccccatcg cctcttcgcg ggtccacccg
     2221 aagatagccg cagcttgggc attccattcg gtcacgatgc cggctgcatc aatggtgatg
     2281 actccgtcaa gcgcggtatc gacaatcgcg cgattgcgtt cttggctttg cagtaaggcc
     2341 gcctccgcag cccgcgccca tgcgctttct tgttgggctt gccgatactc cgctcgcaat
     2401 cgaatcgtcg accacaacag caacatcaac agcggaagcc agacggcagc cacgatgctg
     2461 cgttcgacat ggccgagcag aaaggtcccc acgcccaaag taaacagcgt gatcaagacc
     2521 atcacgaacg tcaaccactc atatcggcga gccgttgaag cggttcgctc ttccggttgg
     2581 gtcatgtggt caccttgtga tcacccaggt gtccaggatt gcggatgggc gataagccga
     2641 cttggccgca cgaagtcccg gaagacccgc atcgtccatg gcattgataa agaccgcccc
     2701 ttgcgccatg gcggttcggc aggtgtcgcg gaagagccac tgagccagac cgggaatgga
     2761 acggtctgcg acctccaaca agatgcacca ggtttgagga gtcaaccagt agccgaaggt
     2821 ataggcgaca atgtcatgat tgactctcag gacagtaccc gacaagccga tccgttcatg
     2881 ctcttgaaag acgagcgcgt gggccatttc tgcatcctcc agaagcagct tcccgacctg
     2941 atcgagattt ccccggtgtt tctgctcggc ccaccgccga tacagacgcg cgcagccgga
     3001 atgatcgtca gcccgatagg gttctttcgt gatcgcctgt tctcgggccg cacgattgca
     3061 gagcgcccgt tgtgacttat agtgatctcc tgccaaggca gccaaagcct cgacagggta
     3121 gagatagtct ccttctttcc gacggaatcg aatcccgtcg cactccagca attgtcgttg
     3181 ggactccatc acgttttcga ttcgactcac cggcgacgtc ccgttccacg accgcatcag
     3241 cgcgaacgct tgctccaccg cccgatccaa aggaccggct ccgagcggcg ggagcggcat
     3301 gaaccacccg tccggagact ggacaaaaag aaacaacgtg cgatcgaact ccatccacca
     3361 gtagggaagc agcgatgacc atatgtaatg gtaaggaaac gcaaacgcgg cgggtgtctc
     3421 agggcagagt acctcggccc gttcgagcgc cttctcgaaa tacgagccgt ccgatggtgt
     3481 cagtggatgg agccgaaaat caacccgttg cggcccgaga cgagccgtca ggtgaggcag
     3541 cgtcttgagc acgaccacat cgtcttggaa ggaaccaatg aagcggggat gagccgcaat
     3601 cgtctccacg atcgcttcac ccgcaagaaa gatcgcgaca cgttctgcat attcgcgaat
     3661 cgcgctcgag ggtgcgtcgc gcatgaacgg acacttggaa tcccaaccaa gcagaacttc
     3721 tcgacccgac aggtcccaca tcagcgccat cgggtacagc cgacaatcca aggggcgaac
     3781 gtcgtaaatt ccgcaccggc ccgacttcga atcaaaagcc ggacagagat acccttcacc
     3841 gatcgggttc ttgacaagac caacctgcga gccgaactta tcgggaaaga acgattcggg
     3901 gattccatgt tccacggcat cgctgatttc cgaacgtgta aagtatgggc gaagaaaact
     3961 atcggcttcc ggaaaacgac aacacacatc acaacgaaaa cacgccgaat ccggaaccag
     4021 ttgaggcaga accctcaatg tctgtttgga atccactgtc atgaggtcca ttgagatccc
     4081 aatcatacga tgcatgccga gctgtagcaa ggaaggttcg cttccgatca tctcgcgatg
     4141 tgtccatcct catatccatc gggcagccgg ccgccccttg tcggtctccg atgtgcatgc
     4201 tagcatgagc ttctttgccg gacgctctca caatagcccg cagcatgcta agggattttc
     4261 cgttgacaca acctgaatct tcctgcccga cgctctcttc tgaggcagca cgcgaccgct
     4321 gcatagccat tcggacacgg ctacaggacg ccagtctgtt cggtgtgact gcggcgtggc
     4381 gggtcagccc ctgtccgttt tatctgtctt ccgatcagct gcaatttttc atcaatctgg
     4441 ggccgcaact gctctccttc taccgagcgc tgaatcggct ctacaatgag agcatcaaag
     4501 gaattcaacc ggtctggata tcaacatatc tcgatcaagg caagccggac gccttggtcc
     4561 agtacagccg gatgaaacgg tttcgcgagg ccttgcccac cgtcattagg cccgatgtca
     4621 tccccacaca ggacggcatg gtcattacgg agctggattc ggttccaggc ggcatcgggc
     4681 tcaccgcctg cctctcgtac agctatcatg accttgccgg cggtgattca caactcatcg
     4741 gggggcgaga cggaatggta cgcggatttg cgcgcatgct gcgatctgtg cagggccagc
     4801 gtgagggatg cattgcgatc ctggtctcgg aggaatcaaa agactatcgg ccggaaatgt
     4861 cctggttggc cgctcaactc cgcgaagcgg ggatgcccgt ttggtgtgtc gcacctcatg
     4921 agattcgatt tacggaggaa ggccttcgat tacatatcga cggcagcgag cgagcgatcg
     4981 gtgtggtcta ccgtttctat gaactgttcg acctgttgaa cattcccaag gccgaactcg
     5041 ttcaatatgc cgcgaagaaa ggccggctat ccgtgacgcc accctacaag ccgacactgg
     5101 aagaaaaatc cgcgttcgcg ctgttgcacc atccgaggct ccgtcccttc tgggaaaagg
     5161 aactcggagc ggacggcatg cttcatgtga gcgcaatcat gccgaagacc tggctgctcg
     5221 acccggcgcc gcttcctgcg attgcgacga tccccggcct acggatcgga gggcaagcgg
     5281 ttacaaattg gtccgaactc gagacggcga cgcagaagga gcgtcatttc gtcatcaagc
     5341 cttccggttt ttccgcactc gcttggggaa gccgcggcgt gtcgatcggg catgacatgc
     5401 ctcagagtgg atgggttgag gccctccgga acgcgcttgc atccttcccg gccacaccct
     5461 atatcttaca agaattccac aaggggcggc tgttcgaaat ggactatctg gatgaaggca
     5521 tgcagacgat cgtgcgcatg tccgggcgag cgcggttgtc tccctattat gttgtctcag
     5581 acgataaggc ggaactcacc ggcattctgg cgacgatctg cccggccgac aagaagatcc
     5641 ttcacggcat gaaggacgcg attatggttc cttgcgccct acgatcgaac tgatccgtga
     5701 ttctccaata aaccataaaa tgggcgtttc actttcttcc atttcctggt agcctaatat
     5761 gattgtggct atgactcttt tccatgtcga ctggtgccct gactgtcacg tggtccgcca
     5821 aaaactggct gacttgggag tgacctacga atctgtcgtc gtgcccgaca gcagacggat
     5881 gcgcacgcag gtttacgagg tctccggcca atactatgtg ccggtcctca aggacgacga
     5941 tctcgttctg acggaaaccg acgacattct ggcctatctg gacgagcggt atagtgccga
     6001 ccgcgcagaa gccgacacag caaccgagtt tcagtcacaa gcccgtacga cgcccgaggt
     6061 ctcgggcaaa gacgacgagc acccttcctg ccgtattaac tgatcaacaa aggacgattc
     6121 atggaagact ggagacaagt cctggcccgg agcattgtga agcccaggga cttggccgaa
     6181 cggttcggtc tcgatgagaa agagatcgaa gcgatcgtcg gcccttatcc catgcgaatc
     6241 accccgacgg tactcgagac aatcaaatcg aaggatgacg cgatctggaa acaggttgtc
     6301 cccgatcccg cagagctgaa cgacattgcg gccgacgacg atccccttga agaagatctc
     6361 atgagcccgg tgcctcacct cgttcaccgc tatccggacc gggtgctgct catggtgacg
     6421 aatcaatgtc cgatctattg ccggttttgt acgagaaagc gactggtggg aaaacccggc
     6481 ttcctcaaac aaggcgagct ggaccgagcc attcagtacc ttcgcgagca caacgaggta
     6541 cgcgatgtca ttctctcggg aggggatccg ctgctgcttc ccgaccacct tcttgagcgt
     6601 atattgaagg cgcttcgaac gatccctcat ttggagctca tacggatcgg ctcgcgggtc
     6661 cccgggtcat taccccaacg gatcacgtcc aaactgtgtg agatggtgaa aaagtaccat
     6721 cccatttaca tgaacctgca tttcaatcac ccggatgagc tgactcccga ggtgaaagcc
     6781 gcctgcggca tgctcgcgga tgccggtatt ccgctcggtg cccaaacggt tctcctcaaa
     6841 ggcgtgaacg acgatccgga aatcatgaag cgactcgtcc atcaattgct cctcgcccgg
     6901 gtgaagccct attatctgta tcaggcagac ttaaccaagg gaacgaatca tttccggacg
     6961 accgtcgaaa caggcctgaa catcatcaaa gcgctccagg gccatacgag cggaatggcc
     7021 gtgccccatt tcgtgatcga tgcgccgggc ggcggcggca aaatcccatt gctgcccgac
     7081 aactacgtgg tccatcttga tgaggatggg gcgatcctcc ggaattatga ggacaagaca
     7141 ttccactatc ctcagcctaa gtccgataat cggcgggagc ttccgatgat cggtgtacag
     7201 tccccgctcg atgatccgga agaagcctat gcggcttgca gcgacagctg ccctgatcga
     7261 gacggttatg ccgcctgggg aaacagctgc ggcggagatg cggacgacct gtgaccactc
     7321 attcgccgcg agccggtatt ctcccctatc aagacatcaa acgcttgatc gcaagtcgag
     7381 ccatcagcgc ttctccaaca gtcgaagaaa gacagatcca gccggccagc ctcgatcttc
     7441 gtctgggccg caaagcctat cgactgatca gcagtttttt gccggaactg tcggccatct
     7501 cctctagact ggacgtgctg gatttttatc aatcggatct cgtgatgtac gagatggatc
     7561 tgaccgacgg ggcgattctg gagaagggtc atgtgtatct cgttccgttg cttgaacaac
     7621 tcgccctccc gaagaccatc cgcgcccggg cgaatccaaa gagcacgacg ggccgtctgg
     7681 atgtcttcac ccgcgtggtg acggatctga ccgccggatt cgatgaaatc cgtgccggct
     7741 atcgaggcca gctcttcctg gaagtcgtcc cacgctcgtt tgcgatcaaa gtccgcacag
     7801 ggcagtcgct gaaccagatc cggtttgtgc gcggcgaggc tacggtgccg gaccaagcac
     7861 ttcaggcgct gcatcgacac acgccgttgc tgtatcacaa tacggcatcc cataagaccg
     7921 tagacaaccg agactttcgt gtcgagcgcg gactgttcct ccgcattgat cttgccggca
     7981 cggatcagcg cgatacccgt gtgatcggtt accgggcaaa gaaaaatagc catgtcatcg
     8041 atctcgagaa agtcggccat tatgctgcgg ctgatttttg ggaaccgctc catcgccatc
     8101 gccacgatag cttgctcttg gagccggaag agttttatat ccttgcctcg aaggagcgca
     8161 ttcgtgtgcc ggccggctat gccgccgaaa tggtggccta cgaagcagcc tgcggagagc
     8221 tacggactca ctatgccggc tttttcgacc cggggttcgg ctatggatcg aagggagaga
     8281 tcaaaggcac ccaggtggtg ctggaagttc ggccgcatga cgtgccattc ttgattcatg
     8341 atggacagac ctttttcaag gtcgtctatg acaagatgat agggatgccg gcccagctct
     8401 atgggtcccc gttgggatca tcctatcagc gacaaacctt gacgctcagt aaacatttta
     8461 aagtctagca ggatgttgaa aaagcccgcc agcttcgttc tcgcgtcgct cagagtctca
     8521 acgtaccgga gcgtacgcct cgcctcttcg ctcgctgcgg cctcgctgga cggccttttt
     8581 gaacatcctg cgaaactatg tggagactgt taaccatggc tccgtccgat ctgccacctc
     8641 aaggcgaacc tccacgctcg tcacgtccgg ccaaagcggg agagcaggga ggaggtggga
     8701 acgagcatca catacacgtc atcggcttga tggtcggatc ggcgctgatg tttatcggct
     8761 ttctcaatgt gtttctgtcc atcagcggcg gcttcgaaat caatgtcatg ccgtttctcg
     8821 tctatttcgg cggccttgcg atctgggcca atgccgccat cgaaactccg gctctccgct
     8881 atggaaccat ggccattgca atctccttgg ccttaggcct ctttcagtat ggagaagttc
     8941 tcttttggca caagcaggtc ctcttctggg tcaccatcat cgtcgtcatg tatttcatgt
     9001 tcgtcgagcc caagaaaccc acaccgtgac ctccacggtg acgatctccg tcatcattcc
     9061 ggctctgaac gaagagaaga ccatcagg
//
LOCUS       DATMTI010000945         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_417471, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000945 DATMTI010000000
VERSION     DATMTI010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417471"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..443
                     /locus_tag="VN039_14950"
     CDS             99..443
                     /locus_tag="VN039_14950"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWV47296.1"
                     /translation="MVQSARSASTVQAIIVEMVRRIVERFHPERIILFGSHARGTAGP
                     HSDVDLLVVMQPQGSKRRRAVEIHGLLAGMGVPKDIIVVTPEEFEAYRDAPGTVIRTA
                     WQEGKTLHDRAA"
     gene            427..837
                     /locus_tag="VN039_14955"
     CDS             427..837
                     /locus_tag="VN039_14955"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HWV47297.1"
                     /translation="MTVRPKDEDLVRAWVLKADHDWLNIENNLAAREIPWDTVGFHAQ
                     QCAEKYLKALLISRQINPPKIHDLTELYALLPDGLLVDFDTRLLEELNPTRLKGDTLE
                     SGRPVEQAEALRAVEAARTIREAIRRVLPSACIV"
     gene            complement(901..>2038)
                     /locus_tag="VN039_14960"
     CDS             complement(901..>2038)
                     /locus_tag="VN039_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWV47298.1"
                     /translation="LLNQGGSGRETQTIMLEPTMNPSLLNVASETAAASVPPVDFLEH
                     WTTDCREVKTFRGHSHGIWAVTFSPDGSMLASGGAERLVRMWDIETGRLLRSLRGHTN
                     DIRAIVFTPDGQALVTGSEDRTIRLWNGKTGEPMKLLFSRYDHNVCSLSLSPDGLMLA
                     RGSHNKDIKIWEVTTGTELMTLLGKDEFDHHWSVCVAFSPDGVHLASGSDIGKIRIWE
                     VLPSGEEKVLHNGHWREDQEDTTETRGYFIADDGGFQKPMDYWIGAMTFTPDAKLLIT
                     GSRDATIKLFEMPNAVEKKTLTGHKDWVRCLAVSPDGKVLVSAGDDQTIKFWDLATGR
                     NFRTVKGHSGPVRCVTFSPDGKRFASASWDRTVKLWEGGAKTEE"
BASE COUNT          425 a          550 c          587 g          476 t
ORIGIN      
        1 gctctgactc tgggggccga agcgggtgtg aatagctcct tgtagatgat ttctgaaggc
       61 taaaggagta ttctccggct caagagttca agaataagat ggtccaatca gcaaggagtg
      121 caagcacagt tcaagctatt atcgttgaaa tggtgcggcg tattgtcgag cggttccatc
      181 ctgagcgaat cattctcttt ggatcacatg cccgcgggac agctggtccg catagcgatg
      241 tcgatttgct tgtcgtgatg cagccacagg gttctaaacg gaggcgagcg gttgagatcc
      301 atggattgtt agccggcatg ggcgttccta aagatatcat tgtcgtgact cctgaagaat
      361 tcgaagcgta ccgagacgcg ccagggactg tgataagaac ggcgtggcaa gaaggaaaga
      421 ctctgcatga ccgtgcggcc taaggacgaa gatcttgtcc gggcctgggt cctaaaggcc
      481 gatcatgact ggctcaatat tgaaaataat ctcgctgcgc gggagatccc gtgggatacc
      541 gttgggtttc atgcacagca atgcgcagag aaatacctca aggcccttct tatttctcgg
      601 caaatcaacc ctccgaagat tcatgatcta acggaactct atgcgctact gccggatgga
      661 ttgctggtag acttcgacac gcgcttgctc gaagaattga accctactcg attgaagggc
      721 gataccctgg agtctgggag gccggttgaa caagcagagg cacttcgggc tgttgaggct
      781 gcgagaacga ttcgcgaggc gatccgccga gtcctccctt cagcctgtat tgtctgacta
      841 tacagagtca gaagagagat tctagtggca gccggttttg ctacgggctg ttctctcctg
      901 ctactcttcc gtctttgctc ccccttccca cagcttcacc gtccggtccc atgacgcgct
      961 ggcaaagcgt ttgccgtctg gggaaaaggt gacgcagcgc acggggccgc tatggccttt
     1021 tacggtcctg aagtttcggc cggtggccag atcccaaaac ttgatcgtct gatcgtcacc
     1081 ggcgctgacc aacacttttc cgtccgggga caccgcgaga cagcggaccc agtctttgtg
     1141 gccggtgaga gtttttttct cgaccgcgtt cggcatctcg aacagcttga tggtggcgtc
     1201 tcggctgccg gtaatcaaaa gtttggcatc cggggtgaac gtcatggcgc cgatccaata
     1261 gtccatcggt ttttggaatc cgccgtcatc cgcgataaag tagccgcggg tttcagtggt
     1321 atcttcctgg tcctccctcc aatggccgtt gtgcaacacc ttctcctcgc cgctcgggag
     1381 cacttcccaa atcctgatct tcccgatgtc gctcccgctg gcgagatgaa cgccgtccgg
     1441 tgaaaacgcc acgcagaccg accagtggtg gtcgaattca tcctttccga gcaatgtcat
     1501 cagctcagtg ccggtggtca cttcccaaat cttgatgtct ttgttgtggc tcccgcgcgc
     1561 gagcatgagg ccgtccggtg agagcgagag gctgcagaca ttatgatcgt aacgtgagaa
     1621 caacagtttc atcggctcgc cggtcttgcc gttccacaat cgaatcgtac ggtcctcgct
     1681 gccggtgacg agggcctgtc cgtccggagt gaagacgatg gcgcgaatat cattggtgtg
     1741 gccgcgcaag gaacgcagca accgaccggt ttcgatatcc cacatgcgta cgaggcgttc
     1801 ggcgccgccg ctggcgagca ttgatccatc aggggaaaaa gtgaccgccc agattccatg
     1861 cgaatggcca cggaacgttt tcacttcccg gcagtcggtg gtccagtgtt ctaagaaatc
     1921 gacgggcggt accgatgcag ctgccgtctc ggaagcgacg ttcagaagcg aagggttcat
     1981 tgtgggttcc aacatgatgg tttgcgtttc tcggccgctt cctccttgat tgagcaac
//
LOCUS       DATMTI010000946         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_713502, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000946 DATMTI010000000
VERSION     DATMTI010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713502"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..>1565)
                     /locus_tag="VN039_14965"
     CDS             complement(217..>1565)
                     /locus_tag="VN039_14965"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWV47299.1"
                     /translation="ELLAKKCDYVIEPFIRDVAIEFATRVSMNARLAAGGQEPAASPE
                     QNQTPVGKVGTPFGSSVLRFKAMLLDENSNLIFESGEHVRVRVDVVNTGTRSVENVSA
                     SLSGTSSIIEQFPAVILSIQSLQAGQTKSLEFIATLPPTKLVQQAVIHVTVAESGGAA
                     APAQTLSLTIQPAGTGTDDVNQIPAPAQDFHQPQTFLVSIGVGAYRDPKLTRRRYAVT
                     DAETVAGYFQSLGGIPPSNIRLLQNHKALRSDINEALFGWLPLHATENAVVIVYFSGQ
                     AMVTPTGDILLTPYDGSATDMARLYLLNDLESAFARLKAKQVIFLFDGMVSRLRGDAK
                     GKTASPRWELGGGNIVRLIGGEDFTKGLEDDQHRHGLFTYYLLKGLRGEADTNRNGIV
                     TFGELAGYMRQKVAWAAKSQFNQEQRPLLLPPLKLDDPAASLVLTVLPSLTSSEVP"
BASE COUNT          323 a          415 c          470 g          357 t
ORIGIN      
        1 ctggagtcct gcctcctgcc gcagttcctt aggaatcacc aaacgaccgg cagcatcaat
       61 ggttgttttc atggtatgaa acataccatt actctcccca ttttgtaaac acttgggatg
      121 cgcattcctt gtcggaacga attgctattg cgtgaacatg tactgcgatg atccgcctga
      181 cgcccgtttg tccgcgcacg tcatgaatcc tggggcttac ggcacttcgg aagaggtgag
      241 tgaaggaagc acggtcagga cgagtgaggc ggccgggtcg tccagcttga gcggtggaag
      301 cagcaagggc cgctgctcct ggttgaactg agatttcgcc gcccaggcga ctttttggcg
      361 catgtagccg gcgagttctc caaatgtgac gatcccgttg cgattagtat cggcctcgcc
      421 tcggagtcct tttaagaggt agtaggtaaa caggccgtgg cgatgctggt cgtcttccag
      481 ccccttcgta aaatcttcgc ccccaatcaa tcggactata tttcctccac cgagttccca
      541 gcggggtgaa gcggtcttcc ccttggcatc accgcgaagt ctggacacca tgccgtcgaa
      601 caagaagatg acctgtttgg ccttgagcct ggcgaaggct gactcaagat cattgagtag
      661 gtagagtcgg gccatgtcgg tcgcactgcc gtcataaggg gttagcaaga tatcgccggt
      721 cggtgtcacc atcgcttgtc ccgaaaaata gacgatcacg acggcattct cggtcgcatg
      781 cagtgggagc cagccgaaca aggcctcgtt gatatcacta cggagggcct tgtggttttg
      841 taacagccgg atgttggacg ggggtatgcc gccgagcgac tgaaaatagc cggcgacggt
      901 ctccgcatcc gtcacggcgt agcggcggcg tgtgagcttg ggatcgcggt aggcgccgac
      961 accgatagag acgagaaagg tctgtggctg atgaaagtcc tgcgccggcg cggggatttg
     1021 gtttacgtcg tccgtgccgg taccggccgg ctgaatggtc aacgaaaggg tttgggccgg
     1081 tgccgcggct cctcccgatt cggcgactgt gacatggatg acggcttgtt ggaccagttt
     1141 ggtcgggggg agggtggcga tgaattcgag ggatttcgtt tggcccgctt gtagtgactg
     1201 gatcgacaat atcactgcag gaaattgctc tatgatcgac gaggttccgc tgagagaagc
     1261 cgacacattc tcaaccgaac gcgttcctgt attgaccaca tccacacgaa cccgcacatg
     1321 ctcgccgctt tcaaaaatca gattactgtt ctcgtcgagc agcatggctt tgaatcgaag
     1381 aactgaagat ccgaaaggcg ttcccacctt tccgaccggc gtctggttct gctccggcga
     1441 agcagcgggt tcctgtcccc cggcggctag tctggcattc atggagacac gtgtcgcaaa
     1501 ctcgatggcg acgtcgcgga tgaaaggttc gatcacataa tcgcactttt ttgcgagtag
     1561 ttcga
//
LOCUS       DATMTI010000947         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_34945, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000947 DATMTI010000000
VERSION     DATMTI010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34945"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VN039_14970"
     CDS             <1..831
                     /locus_tag="VN039_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47300.1"
                     /translation="LWDVEVNELLVEGQGVSFLGVECYTKAPVLRDWATWTLSVTNGE
                     SCNASAVVRAWTKHSGRALIGRLSLRVPSWEVFGSNPYGTAGISYDVDYASTDVRRMM
                     HVGDKTFVETSRIEGRDSVPQITGWIMAECRVRLWWAMCAAGFDNVAHVDTDSVLVNG
                     EGLARLKAHYGASFKHMWQVKATWNYMTVYGPRNLRAGRDRKVAGVPKGAKEVEPNVF
                     TGERWRGLAADMASGRADAVTIVPARWEMNADDPRRRSDPADAGRTVAIRVDLNGYVP
                     "
     gene            complement(838..1128)
                     /locus_tag="VN039_14975"
     CDS             complement(838..1128)
                     /locus_tag="VN039_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47301.1"
                     /translation="MAGKSIDKFVVMAAGKIDGKTHVWTAAVFNSVKDAKPWMALLNL
                     ARKSADAETVKAMDVHAPAVPEGKELKDVKYSGATIQYAPEAAGLSDDATLG"
     gene            complement(1208..1378)
                     /locus_tag="VN039_14980"
     CDS             complement(1208..1378)
                     /locus_tag="VN039_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47302.1"
                     /translation="MRVVKYSTHLWAIQKVSTGALVKAYDEYTGKDEVVVFSSHLQAR
                     FWGAEQGFEMVR"
     gene            complement(1445..1627)
                     /locus_tag="VN039_14985"
     CDS             complement(1445..1627)
                     /locus_tag="VN039_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47303.1"
                     /translation="MTFDDLMELVLQASHSIQNMGFNVCEVNVIELNTRNDPPTLILE
                     TKETADTPPIRLISML"
     gene            complement(1629..1859)
                     /locus_tag="VN039_14990"
     CDS             complement(1629..1859)
                     /locus_tag="VN039_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47304.1"
                     /translation="MNLPEGPSSIVARPGLDAIMATVNLLNAPQRSQILIYVTSYLTT
                     VSTGRPIDELVVREAYGSAFIMFHVDPNLAGI"
BASE COUNT          400 a          536 c          650 g          459 t
ORIGIN      
        1 ctgtgggatg tggaagtaaa tgaactcttg gttgagggtc agggcgtatc atttctcggt
       61 gtcgaatgct acacgaaagc gccagtactt cgggattggg ctacgtggac gctatctgtc
      121 actaacggtg aatcgtgcaa tgcgtcagcc gtcgtacgtg cttggaccaa gcattcgggc
      181 cgggcgctca ttgggcggct atcactgcgc gtaccatcat gggaggtatt tggtagtaat
      241 ccgtatggga cggcaggtat ttcctacgac gtagactatg cgtcaacgga tgttcggcgc
      301 atgatgcacg taggggataa gacctttgtg gagacgagta ggattgaagg gagggacagt
      361 gttccgcaga ttaccggctg gattatggca gagtgtcgcg tacgcctttg gtgggctatg
      421 tgtgcggctg ggtttgataa tgttgctcat gtcgatactg attctgttct cgtaaacggc
      481 gaaggattgg cacggctaaa agcacactac ggagcaagct tcaaacacat gtggcaggtt
      541 aaagccacgt ggaattacat gacggtgtac gggccgagga acctcagggc ggggcgtgac
      601 cgcaaggtgg caggggtccc taagggagcc aaggaagttg agccgaacgt gttcacgggt
      661 gagcgctggc gcgggctagc ggccgacatg gctagcggta gggctgacgc cgtgacaatc
      721 gtacctgcaa ggtgggagat gaacgcagac gacccgcgcc gccggagtga tccggctgac
      781 gcgggccgca ctgtggccat ccgtgtggat ttgaacggct acgttcccta ggaacgatta
      841 gccaagcgtg gcgtcatcac tcagaccggc cgcctcgggc gcgtactgaa tggtggcgcc
      901 actgtacttg acgtccttaa gctccttgcc ctcaggcacg gccggagcgt ggacgtccat
      961 ggccttcaca gtctccgcgt ccgccgactt ccgggccagg ttcagcagcg ccatccacgg
     1021 cttcgcgtcc ttcacggagt tgaacacggc ggcggtccag acgtgcgtct taccgtcaat
     1081 cttgccagcc gccatgacga cgaacttatc gatgctctta ccggccattt cgggccacct
     1141 ttccaggtta atggtctcat caggccggac cctatccggc gacgccgtga ggcgtttcga
     1201 cctttggcta tcgtaccatt tcaaagccct gttcggcacc ccaaaagcgc gcctggaggt
     1261 ggctggagaa tacgactacc tcgtctttcc cggtgtattc gtcgtatgcc ttgacgagcg
     1321 cgcccgtgga cactttctga atggcccaca gatgagttga gtacttgacg acgcgcattg
     1381 tagtgtctcc cttggtctcg tcagtacggc attgagccgt agacgcctta cggcgttttg
     1441 acctttagag catggagatg agacgaatgg gcggggtgtc tgccgtttcc ttggtctcca
     1501 ggatcagggt gggcgggtcg ttcctcgtgt tgagctcaat cacgttgact tcacacacgt
     1561 taaagcccat gttctgaatg gagtgcgagg cctgcaagac cagttccatt aggtcatcaa
     1621 aggtcatgtc agatacccgc caggttcggg tcgacgtgga acatgatgaa tgcggaaccg
     1681 taggcctcgc ggacaaccaa ctcatcgatc ggccgtccag tgctgactgt cgtcaggtag
     1741 gacgtgacgt agatgagaat ctgggagcgc tggggggcgt tgagcaggtt gaccgtcgcc
     1801 atgatggcgt ccagtccggg acgcgcgacg atagatgagg gaccctcagg caggttcatc
     1861 gcttggctcc ttccttgctg ttgttatctt gtagtaccta tactagcgca tccgtggaca
     1921 gctgcaaggg tggcggacgc gtcaattcct atcgttatca cgccgtgatg tagatggaga
     1981 accagagtgc tgacgtcaag ccgttgcgac cgcccgttcc ctcctttcgc ggggcgggcg
     2041 gggcg
//
LOCUS       DATMTI010000948         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_248888, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000948 DATMTI010000000
VERSION     DATMTI010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248888"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VN039_14995"
     CDS             complement(<1..758)
                     /locus_tag="VN039_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV47305.1"
                     /translation="MAGTQSPILVTGAAGFIGFHVAMRLLDRGDQVIGLDNINDYYDV
                     RLKQARLAQLTPHERFRFVKLDLSNRQGMRDLFAGESIQRVVHLAAQAGVRYSLANPH
                     AYTESNIEGFINILEGCRRCNIEHLVYASSSSVYGGNTHMPFSTHDNVDHPVSLYAAT
                     KKANELMAHCYAHLYQLPCTGLRFFTVYGPWGRPDMALFIFTKAILEGKPIEVFNQGR
                     MKRDFTYVDDIVEGVIRTLDHPATADPTWSGDRPT"
     gene            complement(783..2414)
                     /locus_tag="VN039_15000"
     CDS             complement(783..2414)
                     /locus_tag="VN039_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47306.1"
                     /translation="MSQMWTSLPKNHYVSLAPWCWVQLESAEPPGPFPFIGGVAPEVV
                     AGLHEAHSLLSSAIDTAISDVFSKRAPLDDPNRRRRLEDAYAELVNARPYLQQHIRCG
                     RRSDGAFQWEFPTDPDKTATVTNGGLRIFHAINHQAIPIGFNGRPLGPSVGKLLGLLD
                     GTHTVDDLRSAVSAMPRDSQMMLTKVMESLHHYGCLATSATTSIRRHWHDVVRDQDIV
                     HLGHAALLYRQRDSALLFDPWLLPWFAESPLPSVWTGLFPKPAAMFLTHDHDDHVDPR
                     TLLHLPKDTPIIVPSRRNRTGLFFDYRSLLAELGFDRVIELAHGETWPFEGGAVVSVP
                     FYGEDPCDLNMPRNCYLISDRGHNVLIHADSGPTNDGRSALKDNAIRQLVGRYGPIPL
                     LFASQQQLLEIRSHAAHASLSHPGKWLDVGENGYLTNAYLAEVCAAAHARMFVSYATG
                     GADWYPDHLSFMFSRRNPCRTALLTAHWEHPEKLKDLLVSQECRYHRAHAFDVYRVLS
                     GGMIEVSSARETLAPMTLYQLDHGDPPFIKAGKGR"
     gene            complement(2411..>2693)
                     /locus_tag="VN039_15005"
     CDS             complement(2411..>2693)
                     /locus_tag="VN039_15005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV47307.1"
                     /translation="ALHQIPKDELPSYRPAKAQLLDHIGVILDSPQAVDQMYGEILPT
                     IESLGGRIAKEPKQHRDGSYSFYFADPDGNLVQALYEPTISKLRFSADS"
BASE COUNT          599 a          711 c          806 g          577 t
ORIGIN      
        1 gtcggccgat ccccagacca tgttggatcg gccgtcgcgg ggtgatcaag cgttcggatg
       61 accccctcga cgatgtcgtc cacataggtg aaatcgcgtt tcatccggcc ctggttaaag
      121 acttctatcg gtttgccctc caagatagcc ttggtaaaga tgaagagagc catgtcaggg
      181 cgtccccaag gtccatagac ggtaaaaaat cgaagaccgg tgcagggtag ttgatagaga
      241 tgcgcatagc agtgagccat cagctcattg gcttttttgg tggcggcata cagtgaaacc
      301 ggatggtcca cgttgtcatg ggtggagaac ggcatatggg tgttcccccc atagacagaa
      361 ctggacgagg catagaccaa atgctcgatg ttacaccgcc ggcagccttc cagaatgttg
      421 atgaatccct cgatgttgct ttcggtgtag gcgtgcggat tggccagtga atagcggacg
      481 cctgcctggg cagcaagatg aaccacgcgc tgtatcgatt caccggcaaa gagatcccgc
      541 atgccttgtc gattggagag atcgagcttg acgaaacgaa atcgttcgtg cggcgtcaat
      601 tgggcgagac gggcctgttt caatcgcaca tcgtagtagt cgttgatatt gtccagaccg
      661 atcacctggt cgccacggtc caaaaggcgc atggccacat gaaatccaat gaatcccgct
      721 gctcccgtga caagaatcgg cgactgcgtc ccggccatgg ctgctcctca aagaaaaatg
      781 gtctatcgtc ccttgcccgc ttttatgaaa gggggatcgc cgtggtccaa ctgatacagc
      841 gtcatcgggg cgagggtttc tcttgccgaa ctgacctcga tcatcccccc actcagtact
      901 cgatagacat cgaaggcgtg ggcacgatga tagcgacatt cctgtgaaac cagaaggtct
      961 ttcaattttt cagggtgttc ccaatgagcc gttaagagtg cggttctgca gggattgcgg
     1021 cggctgaaca tgaaagacag gtgatcgggg taccagtcag ctccaccggt ggcgtaggaa
     1081 acaaacatcc gtgcatgggc ggccgcgcag acctcggcga gataggcgtt cgtgagatag
     1141 ccgttttcac cgacatccag ccatttgccg ggatgggaga gcgaggcatg ggcggcatgg
     1201 ctccggattt caagcaactg ctgttgtgac gcgaacagga gcggaatcgg tccgtatcgt
     1261 ccgaccaatt gtcggatggc gttgtccttg agggcggacc gtccgtcatt cgtcggaccg
     1321 ctatccgcat ggatcagcac attgtgccct cgatccgaaa ttaagtagca attcctgggc
     1381 atattcaagt cgcaagggtc ctctccatag aaggggacgg acacgaccgc accaccttca
     1441 aacggccagg tttccccatg ggccagctcg atgactcgat caaaccccag ttctgcgaga
     1501 agtgacctat agtcaaagaa tagtcctgtt cgattccgcc gactgggcac gataatcggc
     1561 gtgtctttcg gcaaatgaag caaggtgcgg ggatcgacgt gatcatcgtg gtcgtgagtc
     1621 agaaacatgg cggcgggttt tgggaacagt ccggtccaga ccgacggcaa cggcgactcg
     1681 gcaaaccaag gcaggagcca agggtcgaag aggagggcgc tgtcccgttg ccgatagagg
     1741 agggcggcat gtccaaggtg cacgatgtcc tgatcccgaa cgacatcgtg ccaatgtcgc
     1801 cgaatagaag ttgtcgcaga agtggctaga catccatagt ggtgcagtga ttccatcacc
     1861 ttggtcagca tcatctgcga atcacgcggc atggccgaca ccgccgatcg gaggtcatcc
     1921 acggtatggg ttccgtcgag cagaccgagc agtttcccta cgctgggacc caacggtcgt
     1981 ccgttgaatc cgatggggat cgcctgatga ttgatcgcgt gaaaaatgcg tagaccaccg
     2041 ttggtcacgg tcgcggtttt atctggatcg gtcggaaatt cccactggaa ggccccatcc
     2101 gatcgccgcc cgcagcggat gtgttgttga agatagggcc gtgcgttgac cagctctgca
     2161 taggcatctt ccagccgtcg ccggcgattg ggatcgtcga gcggtgcccg ttttgaaaag
     2221 acatcgctga ttgccgtgtc gatcgcactg gagaggagac tatgagcttc gtggaggccg
     2281 gcgacgactt caggggccac tccgcctatg aaagggaagg gtcccggtgg ctccgcgctc
     2341 tccagctgta cccagcacca gggagcgaga ctcacataat gatttttggg aaggctggtc
     2401 cacatctggc tcatgagtcg gcactgaacc ttaacttgct gatcgtcggt tcatacagag
     2461 cctggaccag attgccgtcg ggatcggcaa aatagaatga ataactgcca tcacggtgtt
     2521 gcttgggttc cttagcgatt cgtccgccca gagattcgat cgtcggcagg atctctccgt
     2581 acatctgatc gacggcttgt ggactgtcga gaatcacccc gatgtgatcc aaaagttggg
     2641 cctttgcggg ccggtaagag ggcaactcat cttttgggat ctgatggagc gcc
//
LOCUS       DATMTI010000949         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_545235, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000949 DATMTI010000000
VERSION     DATMTI010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545235"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VN039_15010"
     CDS             complement(<1..808)
                     /locus_tag="VN039_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47308.1"
                     /translation="MHAYGLHPQYSLAVLALLITPTVTYAMSGMEMPHSGHANTSSSL
                     SKNMGEPINSSEAEIEMTYSADGKTVIFVSGRQGSIPSPEVPYNFDIWMAHNVNDEWQ
                     APIHLGPGIDPTVGPNINTSAWELEPSLSDDGNVIYFTRYEPGNLSTGDLYVTQKING
                     VWQPARNWNEVPELPHINTPTGEEHCPIIASDSLIYFNYQQPGVTQDSDIWKVEKKDG
                     VWQKPESLGPRINSPYRDHMHWTGLSKDGKSLIITSTRPDMGSRGGHDEWI"
     gene            complement(1047..2111)
                     /locus_tag="VN039_15015"
     CDS             complement(1047..2111)
                     /locus_tag="VN039_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47309.1"
                     /translation="MMYKRMDHTGSLRRIVFILLLWAISLTLTVAIAAAESLSFLDGH
                     IRFYPAQREGDHILIPFAQPPAGNEEVLLLGHQSDLIHLKHLKRLQKGTINPQSMAHP
                     RPHYVYGLTEGQLSSPRAQFLVAVGQGITPLLKWEADDAMSGKRKWDPTGAMSSDQLA
                     RFSAGPMSSMPDNAETRLSLEDIKASSGPLASTQGGAMSGNQVPLSPCIRPDKGFAIT
                     ALQVLTSKELNTKIYYLQEEGLRQEAILQSTPAMIFDEDKTSEASMKEVVGIVESENS
                     CHVLAYTTSDGYGLHNSGSLKPVGPIAGIIELSTGRAIERWLVLKSAMGAVWGYTFID
                     LPSRPLNHDPQRRFLLEDRG"
BASE COUNT          557 a          689 c          713 g          635 t
ORIGIN      
        1 aaatccactc gtcatgtccg ccgcgcgagc ccatgtccgg gcgcgtactg gtgataatga
       61 ggctctttcc atcttttgaa agcccggtcc agtgcatatg gtcgcggtag ggtgaattga
      121 tacgtggtcc caggctctca ggcttctgcc acacgccgtc tttcttctcg actttccaga
      181 tgtcgctgtc ttgggtgact cccggctgct gatagttgaa ataaatcagg ctgtcggaag
      241 caatgatcgg gcaatgttcc tcgccggtgg gcgtattgat gtgtgggagt tccgggacct
      301 cgttccaatt tctggccggt tgccaaacgc cgttgatttt ctgggtcaca tagagatccc
      361 ccgtcgacag attgcccggc tcgtatctcg tgaaatagat gacattgccg tcatccgaga
      421 gactcggctc caattcccag gcagacgtat taatgttcgg cccaacagtc ggatcaatcc
      481 ccggtcccaa atgtatcggg gcttgccact cgtcgttcac attgtgcgcc atccagatgt
      541 cgaaattgta aggcacctct ggtgagggga tgctcccttg ccgtcctgag acgaagatga
      601 ctgttttgcc atcggcactg taagtcatct caatttccgc ttccgatgag ttaataggct
      661 cacccatatt cttcgatagc gaggaactcg tgttcgcgtg tccgctatgt ggcatctcca
      721 tgcctgacat cgcatacgtc acggtaggag ttatgaggag cgcgagcact gccaaactgt
      781 actgaggatg aaggccatat gcgtgcatgc aattcctccc tgtttcagca taggcgtcaa
      841 ttatatgcga ccgcatctac tgaggtatag gtcggttcac cggcatggat ggaaatatac
      901 gaacctctct tgccactcga agcattttga ggaccttcct tcactcggtc gcgcgcgtga
      961 tgctgagtat ggcaaaatgg cgatgggaat ccgctccgct cggcaagacg agtgacccga
     1021 acacacactc ttcagcatga tggtgcttac ccccgatctt caaggagaaa tcgccgttga
     1081 gggtcatggt tcaacggcct cgacggaaga tcaataaatg tataccccca gacggccccc
     1141 atcgccgact tgaggacgag ccaccgttca atagcccgtc ccgtcgagag ttcaatgatg
     1201 ccggcgatgg ggccaacagg tttcaaggag ccgctattat ggagcccata gccatcactg
     1261 gtggtatacg ccaggacgtg acaggagttt tcagactcga ctatcccgac aacctctttc
     1321 atcgatgcct cggaagtctt gtcttcgtcg aagatcatgg cgggtgtcga ttgcagaatt
     1381 gcttcctgcc gcaggccctc ttcctgcaga taatagatct ttgtgttcaa ctctttagac
     1441 gtcaggactt gaagcgcggt gatggcgaag cctttgtccg gtcggataca ggggctgagg
     1501 ggcacctggt tgccgctcat cgcgccaccc tgggtcgagg ccaatgggcc agaggaggct
     1561 ttgatgtctt ccaaagaaag gcgggtttcg gcgttgtccg gcatcgagga catcggccca
     1621 gctgagaatc gggccaactg gtcgctgctc atcgcgccgg tcggatccca tttgcgttta
     1681 ccgctcatcg cgtcgtccgc ttcccattta aggaggggtg tgatcccctg tcccacagcc
     1741 acgagaaact gcgctcgtgg actcgatagc tggccctccg tgagcccgta cacataatgc
     1801 ggccgtgggt gggccattga ctgtggattg atcgtccctt tttgaagacg tttgaggtgt
     1861 ttgaggtgaa tgagatccga ctggtgaccg agaaggagaa cctcttcgtt gcccgcaggc
     1921 ggctgagcaa atggaatgag aatatgatcc ccctctcgct gcgcgggata gaaccggatg
     1981 tggccatcaa gaaagctcaa ggattctgca gcggcaattg ccaccgtgag ggtcaacgag
     2041 atcgcccaaa ggagcaggat aaacacaatc cgtctgaggc tgcccgtatg gtccattctt
     2101 ttatacatca tgagagtccc tccgggtttg cggtttgcat tggtatcgcc ggtctagacc
     2161 tttggtcaga ttgctcgaaa tcgcagcaca agcatgtttg cgtcttatcc aggtaacgca
     2221 gagtcctcgg gtacaatcga caacggaagg gcatgaccgt acagccggca tcagacacag
     2281 tcgtatatgc aatgcttgag gcgacacgat tcaactgcag cgcagttgaa tcgcgtggaa
     2341 ccttcgttag ctggctgtgc gagaagtctt tcggcgggac catagcgttt agcatttcga
     2401 catgccctac catggtaact gcggaatgcc gacccggtat gggaatccta ttgctcgatc
     2461 atgaattgct gatgatgatg tcatgaagaa atcttcatca ttcatcgacc tgggcttcta
     2521 tattgttgca ggctgtggga gaattctttc gactggacca taagtattca acatttcggc
     2581 aggccctacc tatg
//
LOCUS       DATMTI010000950         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_799892, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000950 DATMTI010000000
VERSION     DATMTI010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799892"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VN039_15020"
     CDS             <1..1089
                     /locus_tag="VN039_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWV47310.1"
                     /translation="EDGPSIAKWLLELGVLFDRQADGNLHVKKGGGSSKPRLLTCSDY
                     TGLEIMRVLKDEVINQKIQLLEFAAAIELLSDDEGNCTGAVFKDLDNQRHVVVAAKSV
                     ILATGGIGRLHIQGFPTSNHYGATGDGLCLAYRMGAKLAHVDTFQYHPSGAVYPEQLI
                     GALVTEGIRSEGGQLVNAKGERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRVGVWL
                     DTPLLDAEHGPGTVEKHFPAMMLQFERFGIDISKDPVLIYPTLHYQNGGVKIDTNSET
                     NVKNLFVAGEASGGLHGRNRLMGNSLLDLMVFGKRSGLTAAGHAKAMKQGKLTLKHLQ
                     RFRAEAKKHGNVSGVISPMILPAYTRKV"
     gene            1202..2380
                     /gene="sucC"
                     /locus_tag="VN039_15025"
     CDS             1202..2380
                     /gene="sucC"
                     /locus_tag="VN039_15025"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWV47311.1"
                     /translation="MNVHEFQAKSLFAQFGIPVPRGKEITSPDAATAWADQLNTPVFV
                     VKAQIHAGGRGKAGGVKITKDKAAVAGLAKELIGKTLVTHQTGPKGRQVHRLLMEEGA
                     NIAKELYLSILVDRDTGWPTFIASTEGGMEIEEVAAHTPEKIIKEAIDPAVGFQGHNG
                     RNVAFAVGLQTIEPSVINQFVKLLDSLYRLFMEKNAALVEINPLIITKEKTLVALDGK
                     VSFDDNGLFKHEDVQKMRDLNEEEPLEIEAATNNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKETVAAGFRILLKDPNVKGIFINIFGGIVRCERIAHG
                     VIEAAKEVKITVPLVVRLQGTNADEGRKLLAESGVKLEVANDLWEAAQKIVRLTGKAA
                     "
     gene            2417..2629
                     /locus_tag="VN039_15030"
     CDS             2417..2629
                     /locus_tag="VN039_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47312.1"
                     /translation="MGKARFQETLEQVGALSEDEQQSLVEIVRHRLVEQRREELAKNI
                     IEAKEDYRRGKVKRGTIETLLRDIKR"
BASE COUNT          653 a          700 c          872 g          536 t
ORIGIN      
        1 gaggatgggc cgtcgattgc gaagtggctc ttggagttgg gtgtgttgtt cgaccgtcag
       61 gccgacggca acctccatgt gaagaagggc ggcggcagtt ccaaacctcg ccttctgaca
      121 tgttccgatt ataccggcct cgaaattatg cgcgtgctca aggacgaggt catcaatcaa
      181 aagattcaat tgctcgaatt tgcggccgct atcgaattgc tcagcgacga cgaagggaac
      241 tgtaccggcg cggtcttcaa agatctcgat aatcagcgcc atgtggtggt ggccgccaag
      301 tccgtcatcc tggcgaccgg cggcatcggc cggctgcaca ttcaggggtt tcccacgagc
      361 aaccattacg gcgcgacggg cgatggactc tgtctggcct accgcatggg cgccaagctg
      421 gcccatgtcg atacattcca ataccatcca tcaggcgcgg tgtatcccga acaattgatc
      481 ggtgccttgg tgacggaggg catccgttcc gaggggggac agctggtgaa tgcgaagggc
      541 gaacgcttcg tgaacgaact cgacacgcgc gacgtcgtgt cttcctcgat catccgcgag
      601 tgcgaagaag gccgcggtat cagaacgatg tccggccgtg tcggcgtctg gctggataca
      661 ccgttgctgg atgcggaaca cgggccaggc acggtggaaa aacatttccc ggcgatgatg
      721 ctccagttcg agcggttcgg aatcgacatc agcaaagatc cggtcttgat ctatccgacg
      781 ctgcattatc agaacggcgg ggtgaagatc gatacgaact cggaaacgaa cgtgaagaac
      841 ctcttcgttg caggcgaagc gtccggtggg ctgcatggac gcaaccgcct gatgggcaat
      901 tcgctgctcg atctcatggt tttcggcaag cggtccgggc tgaccgcggc cggccatgcg
      961 aaagcgatga agcagggtaa gctcacgctg aaacatttgc agcgattccg cgccgaggcc
     1021 aagaaacacg gcaacgtcag cggtgtcatt tcaccgatga tcctgcctgc gtacacgagg
     1081 aaagtgtaag ggagcatatg gcctatggct gatagcgtat ggcaagaaag cccaatcgcc
     1141 gtcagtccgt gccataagcc ataagccatt agccatcagc tattagctct tcgaggtaag
     1201 aatgaatgtc cacgaattcc aagccaaatc cttgttcgct cagttcggca taccggtgcc
     1261 tcgcgggaag gagatcacgt ctccggacgc tgcgacggcc tgggccgacc agctgaacac
     1321 cccggtcttc gtcgtcaagg cccagatcca tgccggagga cggggcaagg ccggcggggt
     1381 caagatcacc aaagataagg ctgctgtggc cgggttggcc aaggaattga tcggcaagac
     1441 tctggtgacg catcagaccg gtccgaaggg acgccaggtc catcgtcttt tgatggaaga
     1501 gggtgcgaac atcgcgaagg agctctatct ctcgattctc gttgatcgcg acaccggctg
     1561 gccgacgttc atcgccagca ccgaaggcgg gatggaaatc gaggaagtag cggcccatac
     1621 acccgagaag atcatcaagg aggcgattga tccggccgta ggcttccaag ggcataatgg
     1681 acgcaacgtg gcgttcgcgg tggggttgca aaccatcgaa ccgtcggtga tcaatcagtt
     1741 cgtcaagctg ttggatagcc tctatcgcct tttcatggag aagaacgccg cgctggtcga
     1801 aatcaatccg ctcatcatca cgaaggaaaa aacgctggtg gcgctggatg ggaaggtttc
     1861 gttcgacgac aacggcctct tcaagcacga agacgtacag aagatgcggg atttgaacga
     1921 agaagagccg ttggaaatcg aggcggcaac caataatttg aattatgtca aacttgacgg
     1981 gaatatcggc tgcatggtga acggtgcggg cctcgccatg gcgacgatgg atgtcatcaa
     2041 actggcaggc agtgaaccgg cgaacttcct ggatgtcggg ggcggcgcca cgaaagaaac
     2101 ggtggccgcg gggttccgca ttctgctgaa agatccgaac gtgaagggaa tcttcatcaa
     2161 tatcttcggc ggtatcgttc ggtgtgaacg gattgcgcat ggtgtgatcg aggccgcgaa
     2221 ggaagtgaag attacggtgc ccctcgtcgt tcgcttgcag ggcaccaacg ccgatgaggg
     2281 ccgcaagctc ttggccgaat ccggtgtgaa gctcgaagtc gccaatgatt tgtgggaggc
     2341 ggcgcagaag atagtgagat tgacagggaa agctgcataa ggagcgggtc ctgacgccgt
     2401 tcagcaggag gcgcgaatgg gcaaggcgag atttcaagaa acgttggagc aggtaggggc
     2461 gctctcagaa gacgaacagc agtctcttgt cgagattgtg cggcatcgtc tcgtggagca
     2521 gcggagagag gagcttgcca agaacatcat tgaggccaaa gaggactaca ggcgaggcaa
     2581 ggtcaaacga ggaacgattg agactttgct tcgtgacatc aagagatgag gcggctcgta
     2641 tggggcaaga cctttcttcg ggcttacaaa aaggcgagcc ggcgccaccc agagcttgag
     2701 gtagatgttg aacgagttct gcaggtgctc gtgcgtgatc cgtttaatcc acaattacga
     2761 a
//
LOCUS       DATMTI010000951         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_502806, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000951 DATMTI010000000
VERSION     DATMTI010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502806"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VN039_15035"
     CDS             <1..1122
                     /locus_tag="VN039_15035"
                     /inference="COORDINATES: protein motif:HMM:NF036552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HWV47313.1"
                     /translation="SSKSKKRTVTDPQFYWWDEPQDIVRLQVSGALGSSDTVITVSST
                     DPTTTDAAKPWGAASHLKEGDLLLAESTDSSSFANEILEVVSVISATQFQVSRGAAGS
                     TAASIADTTKLTLIGSVYAEGSAAPSAVSRNPLKYTNYIQIFKDSYELTGTVLATNTR
                     TGEPWSNDKKRKMWDHSRGIELSMLFGKPSEVIGANGKPKRTMAGLRYQIPSANQYVY
                     SAGTTWFDLIDRMSPLFSYSSKAGDERAAFCGFGALNAINKIIMADANSEIQWQGVEK
                     VYGMNFRVLQMPQGRLLLKTHPLMSRFSGYYTNSMFVLDMSAFTYVTMKGRDTKVVDD
                     VQDKDEDLRRGFIQTDCSVELDMGGVTCGYIGNINYQAA"
     gene            1133..1513
                     /locus_tag="VN039_15040"
     CDS             1133..1513
                     /locus_tag="VN039_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47314.1"
                     /translation="MRQRHDYGLHTGVEDFRRMPRWPVLTVGAAGSTAADAGAIPFSG
                     IIHAIGADATKGVILPAPSGPGDTCFIKNSTAANAVLKVYPPSGKSINDGTADAALSM
                     AAKTSAYFVSLDGAGWVTLPLLPS"
BASE COUNT          345 a          424 c          496 g          419 t
ORIGIN      
        1 agctcgaaga gcaagaagcg cacggtaact gacccgcagt tctactggtg ggatgagccg
       61 caggatattg ttcgcctgca ggtaagtggc gcgttgggtt cgtcggatac ggttattacc
      121 gttagctcga ctgatccgac gacaactgat gcggctaagc cgtggggtgc tgcatcgcac
      181 ttgaaggaag gcgatctgct cttggcggaa tcgactgact cgagctcgtt tgctaacgag
      241 attttggaag tcgtttcggt catttcggct acgcagttcc aggtctctcg tggtgcggca
      301 ggatcgacgg ctgctagtat cgctgatact acgaagctga cgttgatcgg ttcggtctac
      361 gcggaaggta gcgcagctcc ttcggccgtc tctcgcaatc ccttgaagta tacgaactat
      421 attcagatct tcaaggactc ttacgagctg accggcacgg tccttgctac caatacgcgc
      481 acgggcgagc cgtggagcaa tgataaaaag cgcaagatgt gggaccactc ccgcggtatt
      541 gagctttcga tgctcttcgg aaagccgagc gaggtgattg gagctaatgg caagccgaag
      601 cgtactatgg ctggcttacg ttaccagatc ccctcggcga accagtacgt atattctgct
      661 ggtacgacct ggttcgatct aatcgatcgg atgtcgccgc tcttcagcta cagctcgaag
      721 gccggtgatg aacgcgcagc cttctgcggt ttcggtgcgc tgaatgccat taataagatc
      781 attatggcag acgcgaactc tgagatccag tggcagggtg ttgagaaggt ctacgggatg
      841 aacttccgtg tccttcagat gccgcaaggt cgtctcctgc ttaagactca tccgctcatg
      901 agccgcttta gcggctacta cacgaactct atgttcgtgc tggatatgag cgctttcacc
      961 tatgttacga tgaaaggtcg tgacacgaag gtggtggatg atgtgcagga taaggatgaa
     1021 gatcttcgtc gtggcttcat ccagaccgac tgctcggtcg agcttgatat gggcggtgtg
     1081 acctgcggtt acatcggtaa tatcaactat caggcagcgt aaggaggctt aaatgcgtca
     1141 acgtcatgac tacgggctac atacgggtgt ggaagatttc cgccgtatgc cccgctggcc
     1201 ggttctaacg gtaggtgccg ctggatcgac tgctgccgat gcaggtgcga tccccttctc
     1261 ggggatcatt catgcgattg gtgcggatgc tacgaagggc gttatccttc cggctccgtc
     1321 tggtccggga gatacttgct tcatcaagaa ctccacggca gccaatgcag tcttgaaggt
     1381 gtatccgcct tcgggaaagt ctatcaacga tggtacggct gatgccgcgc tgtctatggc
     1441 agctaagact tcggcctact ttgtctctct tgacggggct ggctgggtta cgctacctct
     1501 gcttccgtcg taagctttta cgatggatga gggagaggca tctagcctct cccattttta
     1561 ggaggctata aatgtatcat cgcgggttcg atcctcgtcg tcactactgg ccgatctcac
     1621 gggcgcatac gcccatgggg caggctttga aggctgctct ggatgctctg catcctggag
     1681 atgg
//
LOCUS       DATMTI010000952         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_119564, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000952 DATMTI010000000
VERSION     DATMTI010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119564"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VN039_15045"
     CDS             <1..256
                     /locus_tag="VN039_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47315.1"
                     /translation="GTGVDQPFDDLHNQFHAIDNSICCVEKSVKGQPAYALAVTHNKE
                     LKTIFKRLWKFKNDQPQRDQHLDLFVSELQEMRQTLETLR"
     gene            261..1118
                     /locus_tag="VN039_15050"
     CDS             261..1118
                     /locus_tag="VN039_15050"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV47316.1"
                     /translation="MAGPSPEQVIDSQRQDWNRVAGGWEKWDRFFDEQMAFLNHRLVG
                     DARLHSGLRVLDLGSGTGYPALLAAQAVGANGSVVGIDLAEHMLAVAERKATRLGLQN
                     VMFRVGDVTTLPFESASFDAVTSRFCLMFLPEIPKAAAEIARVLKPGSWLAAAVWSAP
                     EKNPSIGLSMTAVKQVIDLPPTDPMAPGIFRLAQPGDFAGMLERAGLIDITDQEFLGE
                     WSYGSTDEYYTSLMEIAAPVQNLMTTLSEAQRQEVRRLIIKAASQFQRGARITFPIAV
                     RMVAGRKPG"
     gene            1122..1433
                     /locus_tag="VN039_15055"
     CDS             1122..1433
                     /locus_tag="VN039_15055"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWV47317.1"
                     /translation="MPTKNDGFKDFILDQLTELNGVTARAMFGGYGLYQRAAFFGIIH
                     KGRLYFKVSTTTVHNYMDHGMKPLRPNPKQTLKSFYEVPIDVIEDAEFLTQWAIEAAE
                     G"
     gene            1441..1983
                     /locus_tag="VN039_15060"
     CDS             1441..1983
                     /locus_tag="VN039_15060"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWV47318.1"
                     /translation="MSQVLWTIGHSTRSIDEFIALLKAHGIQQLIDVRTVPRSRHNPQ
                     FNHENLSESLKSEDLSYRHMPQLGGLRKPKKDSINMGWRNESFRGYADYMQTNEFQRA
                     IEELMAYGTSKKTAIMCAEAVPWRCHRSLIADALVTRDWEVRHIMSETKADRHKLASF
                     AAVENGELRYPRPDSGSLFP"
     gene            complement(2301..>2523)
                     /locus_tag="VN039_15065"
     CDS             complement(2301..>2523)
                     /locus_tag="VN039_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47319.1"
                     /translation="FDTTQSMSEVSDRFLGQVCLPKRVVHREQGSLSMACNLTLVVPG
                     WCPRNDRAGKKRDKRLKGFERCASSIVSN"
BASE COUNT          607 a          720 c          668 g          528 t
ORIGIN      
        1 aggcactgga gtcgatcaac ccttcgacga tctgcacaac cagtttcatg cgatcgacaa
       61 cagcatctgt tgcgtcgaga aatcggtcaa gggacagccg gcctacgcac tggctgtcac
      121 acacaataaa gaactcaaga cgattttcaa acggctatgg aaattcaaga atgatcagcc
      181 tcagcgggat caacacttgg acctctttgt gtccgaactc caggagatgc gccaaactct
      241 ggaaaccctg aggtaacttc atggcagggc catcgcctga acaagtcatc gatagccaac
      301 gacaagactg gaaccgtgtc gccggcgggt gggagaaatg ggatcgcttc ttcgatgagc
      361 agatggcgtt tctcaatcac cggctcgtgg gcgacgccag attgcacagc ggtcttcgag
      421 tgttggatct gggatcaggc acgggctatc cggcactcct cgccgcccag gcagtcggag
      481 cgaacggcag cgtggtgggc atcgacctcg ccgagcatat gctcgctgta gcggagagaa
      541 aggccacgcg attgggctta caaaacgtga tgttccgggt cggcgatgtg accactctcc
      601 cgttcgaatc cgcttccttc gacgccgtca ccagccgctt ctgtctcatg tttctccccg
      661 aaattcccaa agctgccgcc gaaatcgcac gtgtcctgaa accgggaagc tggctcgcag
      721 cagccgtctg gtccgctccg gaaaaaaatc cctccatcgg tctctcaatg acagccgtca
      781 agcaggtaat cgacctgccg ccgacggacc cgatggcacc aggtatcttt cgtctcgctc
      841 aacccggcga cttcgccggc atgttggaga gagccggcct tatagacatc acggaccagg
      901 agtttcttgg ggaatggtcc tacggctcaa ccgatgaata ttatacgagc ctgatggaga
      961 tcgccgcccc ggtccaaaat ctcatgacga cgctgtcgga ggcacaacgg caggaggtgc
     1021 gacgccttat catcaaagct gcttcgcagt tccaacgcgg agctcgcatc acgtttccga
     1081 tcgccgtgcg gatggtcgcg gggcgcaagc ccggatgagg catgccgacg aagaacgacg
     1141 gcttcaaaga cttcatcttg gatcagctga cggagctgaa cggggtgact gcacgggcca
     1201 tgttcggcgg ctacggtctc tatcagagag ccgcgttttt cggcatcatt cataaaggcc
     1261 gtctctactt caaagtgagc acgaccaccg ttcacaatta catggatcat ggcatgaaac
     1321 cgcttcgacc caatcccaag cagacactca aatcctttta tgaagtcccg attgatgtga
     1381 ttgaagatgc ggaattcttg acccagtggg cgatcgaagc cgccgaggga tgactaactc
     1441 atgtcgcagg ttctctggac catcggtcac tcgaccagat caatcgacga gttcattgca
     1501 ctactgaaag cacatggcat tcagcagctc atagacgtgc ggactgtgcc ccgctctcgc
     1561 cacaatcctc agttcaatca tgagaacctt tcagagagtc tgaagagcga ggatctatcc
     1621 taccgccaca tgccgcaact cggtggtcta cgcaaaccca agaaggattc catcaacatg
     1681 ggctggcgca atgaaagttt tcgcggctac gccgactaca tgcagacgaa tgaattccaa
     1741 agagccattg aagagcttat ggcgtatggc acgagcaaga agacggcgat tatgtgcgcg
     1801 gaagcagtcc cctggcggtg ccatcgatcc ttgatcgccg atgcactagt gacgagagac
     1861 tgggaagtcc gacatatcat gtcggaaacg aaagcagacc gacacaagct tgcttccttc
     1921 gctgcggtgg aaaacgggga actgcggtac cctagaccgg acagtggaag tctattcccc
     1981 tagcgacgtc catgccctgc tcttcttcat cgaaacgggt gaaaagggtt ctatggctcc
     2041 tcggcaggag atttgaagat aggcggctcg atttattttc gacccaaatg ccttatctga
     2101 gtattttttc cgacaagaca gcgatgcctt cggcttgcgc cgcctttcgg aagttcttat
     2161 caaggtggca agcggaagac caagacgcgg ttcttgaagt tgtggcctca tgctcaaaca
     2221 gccacgtcat cgtaactgaa cgatcaagca cgaagttcat cgccacccga cattcttcca
     2281 cgcgagaatg tcttcggtgg tcaatttgac acgatgcttg acgcgcaacg ctcgaatccc
     2341 ttcaatcgct tgtctcgctt cttcccggct cggtcgtttc gcgggcacca gcctggcact
     2401 acgagagtca aattgcaagc catcgagagc gatccctgct ctcgatgaac gacacgctta
     2461 ggcaaacaga cctgtcccag aaagcggtca ctcacctccg acatcgattg agtagtatca
     2521 aac
//
LOCUS       DATMTI010000953         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_713755, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000953 DATMTI010000000
VERSION     DATMTI010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713755"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VN039_15070"
     CDS             <1..485
                     /locus_tag="VN039_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWV47320.1"
                     /translation="TITKENTKEVAMTLVRWDPFRELEEVSDRLNRMFARPAAAGTNG
                     KETMIVADWTPSVDISETEGEYQIKAEIPDVKKEDVKVTLEDGVLTIQGERKHEKEEK
                     GKKFHRIERSYGSFVRTFSLPDVIDEEKVKADFKDGVLNLHLPKSEKAKPKAIEVKVA
                     "
     gene            777..3098
                     /locus_tag="VN039_15075"
     CDS             777..3098
                     /locus_tag="VN039_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HWV47321.1"
                     /translation="MNLFRRCRWTIWPARAVLATLVLACIGSISSAAADINYVYDDAG
                     RLRAVIDPASDMAIYAYDAVGNLTGIMRQSSSTLGIIQFTPSSSSVGTTVTIYGTGFS
                     ATPASNTVKFNGTTATVLTASTTVLTANVPSGATSGPISVTVGATTATSSASFTVGAA
                     PIPTISNFSPSVGTAGTAVTITGTNYDTTPTKNKVTFSPTYSVVATASSTSLSTTVPV
                     GAQSGKIGVTTVNGNVLSANEFFVTPAGVAPADVQYTGRIVVDGSTLTVTISTANKVG
                     LVAFDGVAGQRVGLGLTSVSIPASTVSLYKPDGTLMTYGTYGSGGGVLDSQVLPVTGT
                     YAIVIDPTSTYTGSMTLTLSAELTGTITIAGSPVSFSITRVGQNARYSFTGTAGQTVS
                     LGMTSVTIASSNVSIYKPDGSLLVQPTNITAAGGALDSQVLPVTGTYAILVDPLDLAT
                     GNMTLTLYGTADVTGTITIDGTTVTPTLTVPGQRARYTFSGTAGQWVNLGMTSVTINS
                     STVSILKPDGTTLVSTFIGTSGGSLDPTTTLPTSGTYTIVVDPVGLSTGNMTLTLTSP
                     LTGTIILDGASVPISLTKAGQTARYTFSGTSGQWVSIGFTSVTIASSTFTLLKPDGTT
                     LASNTIGTAGGGLEPPGTLPATGTYTIVVDPSGTNTGTITLMLMSYLSGTLTLDGTLT
                     ASTISTIGQNALYTFTGTTGQWVNLGMTSVSFTSVTVTLWKPDGTPWTWTTVGTSGGS
                     LDAPTALPTNGDLYRHGESHCTVHRHHDAYTLH"
     gene            3076..3411
                     /locus_tag="VN039_15080"
     CDS             3076..3411
                     /locus_tag="VN039_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47322.1"
                     /translation="MTLTLSTEVMGSVTINAAATPITIGRAGQNARYTFVGTASRQTT
                     VKITGNGLGGVSVNLYTPTGTVQTGAGSSATSFNLPTVTLATTGTYTITINPQTTATG
                     SLNLQVTSP"
BASE COUNT          792 a         1031 c          998 g          716 t
ORIGIN      
        1 agacaatcac aaaggaaaac acgaaggagg tcgctatgac actcgtacga tgggatccgt
       61 ttcgggaact ggaagaagtc tcagatcggt tgaaccggat gtttgctcgt ccggctgcgg
      121 caggtaccaa tgggaaggag accatgatcg tggcggactg gacgccgtcg gtcgatatca
      181 gtgagacgga aggggagtat cagatcaagg ccgagattcc ggatgtgaag aaggaggatg
      241 tgaaggttac gttggaagac ggcgtgctca ccattcaggg agagcggaaa cacgagaagg
      301 aagagaaggg gaaaaagttc catcggatcg agcgatccta cggcagcttc gtgcggacct
      361 tctcgctgcc cgatgtgatc gacgaggaga aggtaaaggc agacttcaaa gatggggtgc
      421 tcaacctcca tctgccgaag tcggagaagg cgaagccaaa ggccatcgaa gtcaaggtag
      481 cgtaagctgg tctgagagtt ggctctgaac aaaggcccgc cccatcaggc gggcctttta
      541 tttgactgcc cacatgacac gctgtttcac gcgattgctc atcaaaactc agcgacttcc
      601 ggcaaaacga atacccaagt ggatctaccg gctaaggcca tctttgggga ttgcaagtat
      661 tgagaaacag ggtattgtcc ttccgcataa cgagtcttca catgttgatg gcgacatagc
      721 aggcatgttg gtcctcaaga ggggccaggc agctttaggc aggagcagac acgagggtga
      781 acctgtttcg cagatgccga tggacaatct ggccagcgcg agcggtcctt gcgacactcg
      841 tacttgcatg cattggtagt atttctagtg ccgccgccga catcaactac gtgtatgacg
      901 acgcgggtcg gctgcgcgcc gtcatcgatc cggcatccga tatggccatc tatgcctatg
      961 atgccgtcgg caatttgacc ggcatcatgc gacaatcatc atccacactc ggcatcatcc
     1021 agttcacacc gtcgtcgagt tcagtcggca cgaccgtgac gatctacggc acggggttca
     1081 gcgccacgcc tgcgtcgaat accgtcaaat tcaacggcac gacggcaacg gtcctcaccg
     1141 cttccactac cgtcttaacc gccaatgtcc ccagcggcgc gacgtccggt ccgatcagcg
     1201 tcaccgttgg ggcgaccacc gccaccagtt ccgcgtcgtt taccgttggg gcagctccta
     1261 tcccaaccat atccaatttt tcaccgtcgg ttgggactgc cggcacagcc gtcacgatta
     1321 cgggaacgaa ctacgacacc acgcctacca agaataaggt gaccttcagt cctacttatt
     1381 cagtggtcgc cacggcgtcg tctacatcgc tgagtaccac cgtcccggtt ggcgcgcaat
     1441 ccggcaagat tggtgtgact acagtgaatg ggaacgtcct gagcgcgaac gagttcttcg
     1501 tgacgccggc cggtgtggct cccgccgatg tgcaatatac agggcgtatc gtggtggatg
     1561 gcagcacgct gaccgtcacg attagcacgg cgaacaaagt cggactggtc gccttcgatg
     1621 gcgtggcggg acagcgcgtc ggcctgggtc tgaccagcgt gtctattcct gccagtacgg
     1681 tgtctctgta caagcccgac ggcactctga tgacgtacgg cacgtatgga agcgggggag
     1741 gagtattgga ttcgcaggtg ctcccggtga ccggtaccta tgccatcgtg attgatccaa
     1801 cgagtaccta cacgggcagc atgacgctga cgctgtcggc cgaactgacc gggaccatca
     1861 cgattgccgg atcaccggtt tcgttctcca ttacacgagt gggccagaat gcgcggtatt
     1921 ccttcaccgg gactgctggt cagacggtga gtctcggcat gaccagcgtg acgattgctt
     1981 catcaaatgt gtcgatatac aaaccggatg gcagtctttt ggtccagcca accaacatca
     2041 cagccgcagg cggtgcgctg gatagccaag tgttaccggt cacgggtacc tacgccattc
     2101 tggtggatcc cttggacttg gcgactggga acatgacact cacactctac gggacggcag
     2161 atgtcaccgg cacgatcaca atagacggaa ccacggtgac gccgaccttg accgttcccg
     2221 gtcagcgagc ccgctacacg ttcagcggga ccgcaggcca atgggtgaat ctcggcatga
     2281 cgagcgtaac catcaactcc agtactgtct ccatactgaa accggatggg acgacgttgg
     2341 tttcgacctt cattgggacg agcggtggaa gcctcgatcc caccacgacc ttacccacca
     2401 gtggaaccta cacaatcgtc gtcgaccctg tcggactttc caccggcaac atgaccctca
     2461 ccctcacatc gccgctgacg ggcacgatca tcttggatgg cgcctcagtt ccaatcagcc
     2521 tcactaaggc cggtcaaact gcaagatata cctttagtgg aaccagtggg cagtgggtga
     2581 gtatcggttt tacttcagtg acgattgcgt ccagcacttt cacgctcctc aagcctgacg
     2641 gaactacctt ggcctcaaat accatcggca cagccggcgg tggcctcgaa ccacccggca
     2701 ccctgccggc gaccgggacc tacacgattg tcgtcgatcc gtcgggaacc aatactggca
     2761 ccatcacgct catgctgatg tcctatctct ccggcacact cactctggac ggcacgctaa
     2821 ctgcttcaac catctcgacc atcggtcaga atgcgctcta cacctttacc ggcacgacgg
     2881 gccaatgggt caatctgggg atgacaagtg tgagctttac ctctgtcacc gtcacgctct
     2941 ggaaaccgga cgggacgccc tggacatgga ctactgttgg cacgagcggg gggagtcttg
     3001 atgctccgac ggctcttcca accaacgggg acctataccg tcacggtgaa tcccactgca
     3061 cagtacaccg gcaccatgac gcttacactc tccactgaag tcatgggctc ggtgacgatc
     3121 aatgccgccg cgactccaat cactattggt cgcgccggac aaaacgctcg atacaccttt
     3181 gtgggcactg cgagtcggca gacgacagtc aaaatcacgg gtaatggact gggcggtgtc
     3241 tcggtgaatc tgtatacgcc tactgggacg gttcaaaccg gtgcgggcag ttcggcgacg
     3301 agcttcaatc tcccgaccgt gacgcttgcg accaccggca cctatacgat cacgatcaat
     3361 ccgcagacga cggccaccgg tagcctgaac ctccaggtga cgagcccatg aacactcgac
     3421 aacctctctt cgttgcgatg atggtactgt gtgtcggggt ctttatcgtg atctctgtcc
     3481 tgattagtcc gcccttcagc tggtcaggca atggcgcgaa tgtcgcggga ggttcga
//
LOCUS       DATMTI010000954         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_375701, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000954 DATMTI010000000
VERSION     DATMTI010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375701"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VN039_15085"
     CDS             complement(<1..566)
                     /locus_tag="VN039_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWV47323.1"
                     /translation="MTAPPEHTVTITKGNLALEGILSLPTDSRGVVVFAHGSGSGRLS
                     PRNSFVAHHLQRDRLATLLLDLLTEEEAEDRRKVFDIDLLADRLLLVKTWLETQPQTQ
                     NLGIGYFGASTGAGAALQAAARELSNIKAVVSRGGRPDLAESYLPSVTAPTLLIVGGH
                     DEPVIEMNQAAYDLLTCEKKLVIIPDAT"
     gene            complement(563..2008)
                     /locus_tag="VN039_15090"
     CDS             complement(563..2008)
                     /locus_tag="VN039_15090"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWV47324.1"
                     /translation="MKRLSIRTIVVGIIIVLGLPASWTGAAGPSPSQAAPTPVPMCKS
                     PYKKKSVTAKTLQALVRSHERWVEYRGNPDAKRIELCQANLSQAELVRANLERADLEG
                     TVLRQAKLPQASLAQASLAGADLAKSVLIDSNLSGADLRYARLTGANLLRAIGDEAAL
                     FGAVLIDAKVKGASFERAQLQGADLTSADLTDSNFVEAYFYGATLKDAILVNADLTGA
                     DLRRTILTNANLSRANLERTLLDRAHLEAARLIEADLESAYLDETNLRGANLKRAILR
                     GTDLRYADLQGADLHDADLEAANLEEAALVKVDVHSANLRMAILYHTVLDEADFLDAN
                     LNRAVLIGAKGLHINFMNADLSEIYAPKSSFTHARFNRANLESSNFVGADLRGSNFGH
                     ADMTYADLQDTDLQETNLQHASLAGTDLSGARLDSADLRRTNLKGAILSSVIGLTQTQ
                     LNAACVDDKTKLPPELSRPAPCSPIKKRAKP"
     gene            complement(2027..>3240)
                     /locus_tag="VN039_15095"
     CDS             complement(2027..>3240)
                     /locus_tag="VN039_15095"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWV47325.1"
                     /translation="HRNFLVAAGLEQVLDYLSELRISKEELAWLDQSGRFSSQLLHYL
                     ETLRFTGDVEAMPEGTIFFPHEPIFKIVAPFPEAQLVESRVINLLNFQTMVASKAARF
                     VLAAGGKPLIDFGLRRAHGAEAGLLAARASFLAGFAGTATVLAGMAYRIPLHGTMAHS
                     FVQAHEDEIVAFEHFAQTQPTNVILLIDTYDTEAAADKVVSLALTLKTKGITVKGVRL
                     DSGDLADHARKVRRVLDEGGLPHAQIFASGNLDEYRLRDLIQSRAPIDSFAVGTAMTT
                     SSDAPSLDCAYKLQEYGGRSCRKRSEGKATWPGRKQVYRYLADDGHMDHDIVTTHDDR
                     LPGEPLLQPVMKNGCRLAPWPDLTELQHHTAAQLGQLPESLRALEMVPAYDVRISLTL
                     QSLAQTVDRRL"
BASE COUNT          658 a          887 c          977 g          718 t
ORIGIN      
        1 tgcgtggcat cgggaatgat gaccagtttc ttctcgcaag tgagcagatc gtacgctgcc
       61 tggttcatct caatgacagg ctcatcatga ccgccgacga tcaacagcgt cggagcggtg
      121 accgacggca aataagattc agccaggtcc ggtcgtcccc ctcgtgacac cacggccttg
      181 atgttcgata gctctcgcgc cgcagcttgt aatgctgctc ccgcgccggt gctggcgccg
      241 aagtaaccga ttcccagatt ttgggtctgt ggctgagttt ccagccaggt tttcaccaac
      301 aacagccgat ccgccagcag atcgatatcg aatactttgc gccgatcttc cgcttcctct
      361 tcggttaaga gatccagaag caacgtggca agtctgtctc gctgcaaatg gtgagcgaca
      421 aaactattgc gggggctcaa tcgtccgctc ccgctgccgt gcgcaaaaac aaccacgccg
      481 cgcgagtcgg ttggaaggct tagtattccc tccagggcaa ggttcccttt tgtgatcgtt
      541 acggtgtgtt caggtggggc ggtcatggct tagcccgctt ctttatggga ctgcagggag
      601 ccggacggct gagttcaggc ggcaatttgg tcttgtcatc gacacaggcg gcattgagct
      661 gtgtctgtgt gagaccgatg accgacgaga gtatggcgcc cttgaggttc gtgcgacgca
      721 agtctgctga gtccagccga gcaccgctta aatcagttcc ggctagggag gcatgctgaa
      781 gattggtttc ttgaagatcg gtgtcctgaa ggtcggcata ggtcatgtcg gcatggccga
      841 agttcgaccc gcgaagatcc gcaccgacga aattcgacga ttccagattg gctctgttga
      901 atcgtgcgtg agtgaaagaa gatttcggag cgtatatttc gctcagatca gcattcatga
      961 agttgatgtg caatcccttg gctccgatga ggaccgcgcg gttgaggttc gcgtccagga
     1021 aatcggcttc atcgaggacg gtgtgataaa gaatagccat ccggagattc gccgagtgca
     1081 catccacctt gaccagagcg gcctcttcta ggttggctgc ttccaggtcg gcgtcatgca
     1141 ggtctgcgcc ctgtaggtcg gcgtaccgga gatctgtgcc gcgaagaatc gctctcttga
     1201 gattggcgcc ccgtagattc gtttcgtcca aataggcgct ttccaagtcg gcttcgatca
     1261 gacgggctgc ttccaagtgc gcgcggtcca ggagggtgcg ctcaagatta gcgcgagaga
     1321 gattcgcgtt ggtcaggatg gtgcgacgca ggtcggcgcc tgtgaggtcg gcgttcacga
     1381 ggatcgcatc tttcagggtc gctccgtaaa aatacgcctc gacgaaattg ctgtcggtca
     1441 aatcggcaga ggtgagatcg gcgccctgca actgtgcccg ctcgaacgag gcccccttca
     1501 ccttggcgtc gatcaggacg gcgccaaaga gggcagcttc atcaccgatc gcgcgtaaga
     1561 gattcgcgcc ggtgagccgg gcatagcgta aatcggctcc agagagattg ctgtctataa
     1621 ggacggattt ggcgaggtct gcgccggcga ggctggcctg agccaggctg gcctgaggca
     1681 gcttcgcctg gcgtagaact gtgccttcca gatcggcccg ttcgagattc gctctcacca
     1741 gttcggcctg gctcagattc gcttggcaga gctctatacg tttggcatcc gggttgcccc
     1801 gatattccac ccatcgttca tgcgaacgca cgagagcttg caatgttttg gccgtgacag
     1861 atttcttctt gtagggactc ttacacatag gaactggggt cggtgccgcc tgggagggtg
     1921 atggaccggc cgctccggtc catgaggcgg gaagtccgag gacgatgata atgcccacaa
     1981 caatggtcct gatggacaag cgcttcatct catgtctcct tcgcggttag agcctgcggt
     2041 caaccgtctg agctaagctt tgcaaagtca aggaaatccg aacgtcatag gccggcacca
     2101 tctccagggc tcgcagcgat tcgggaagct gtcccaactg cgcagctgta tgatgctgca
     2161 gttcagtcaa gtccggccac ggagcgaggc ggcagccatt cttcatgaca ggttgaagca
     2221 gcggttcacc cggcaatcga tcatcgtgcg tcgtgacgat gtcgtggtcc atatgtccgt
     2281 cgtccgcaag atatcgatag acttgtttgc ggccgggcca ggtggccttg ccttccgatc
     2341 gcttgcgaca ggaacgtccc ccatattctt gaagtttgta ggcgcagtcc agcgaaggcg
     2401 cgtcagagga ggtcgtcatc gccgtgccga cggcgaagct gtcgatcggc gcgcggcttt
     2461 ggatcagatc tctcagacgg tattcatcca gattgccgct ggcaaaaatc tgagcgtgag
     2521 gcaagcctcc ttcatcgagg acacgtcgaa ccttccgtgc atggtcggcc agatcaccac
     2581 tgtctaaccg tactcccttg acggtgatgc ccttggtttt cagtgtcagc gccaaggata
     2641 cgaccttgtc ggcggcggcc tccgtgtcgt aggtatcgat gagaaggatg acgttggtcg
     2701 gttgagtttg ggcaaaatgt tcaaaggcaa cgatttcatc ttcgtgggct tgcacgaatg
     2761 aatgggccat ggttccgtgc agaggaattc gatacgccat gccggccagc acagttgcgg
     2821 ttccggcgaa tccggccaga aagctcgctc gcgcggcgag gagcccggct tcggcaccgt
     2881 gcgcgcggcg aagcccaaaa tcgatgagcg gctttcctcc tgcggccagc acgaagcgcg
     2941 ccgctttgga ggccaccatg gtttgaaagt tcaacaggtt tatcacacga ctctcaacga
     3001 gctgagcttc agggaacggg gcgacaattt tgaagatcgg ctcgtgggga aagaaaatcg
     3061 ttccctccgg catggcctcc acatccccgg tgaaccgcag cgtttccaga tagtgaagaa
     3121 gctgtgaact gaaccgcccg gattgatcca gccaggccag ttcttccttg gaaatgcgta
     3181 actccgaaag atagtccaat acctgttcga gaccggctgc caccaagaag tttcgatggg
//
LOCUS       DATMTI010000955         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_843088, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000955 DATMTI010000000
VERSION     DATMTI010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843088"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1280)
                     /locus_tag="VN039_15100"
     CDS             complement(198..1280)
                     /locus_tag="VN039_15100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV47326.1"
                     /translation="MRTRMKVIAGSVLILALSSGLTIFSTTSPSQERLNQNALAARPQ
                     SVDVVPVQLRQLQTTARLPGELLPYLAVDLYPKVAALLNWIGVDRGDDVRQGQVLARL
                     VAPELRAQRAEAEAKLRADQITLQHLHTAAETPGVIAVNDLEVAEKTVDAGRARVVAL
                     KEMEDYLIIAAPFDGRVTERQMHPGALLTPGQGKPLLRVEQLGRLRLVIPVPETNAGE
                     IAEGVEVSFTVSAYPGERFTGVVRRIARSVDAKTRTMPVELDVENEKKRLAPGMFAEV
                     EWPVRRPHATMFVPRSAVVTTTERIFVIRVSGDGTVEWVSIQRGEVMGDLQEVFGDLT
                     SHDTVVVHGTDELRPETKVVARSARF"
     gene            complement(1265..4453)
                     /locus_tag="VN039_15105"
     CDS             complement(1265..4453)
                     /locus_tag="VN039_15105"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV47327.1"
                     /translation="MWILFSALRRPVTIIVVIAAVALCAVLALSSMPIDIFPDLGLPA
                     IYVIQPYGGMAPSQMEAYLVSFYEYHVLYILGIEHVESKSIQGAALIKLFFYPGTDMN
                     QALAQTVAYIDRSRAFMPPGTLPPDIIRYDAGSAPVGYLVFHSETRSTGEVQDLALFR
                     VRPLFATLPGVSAPPPFGGNARTVVIHIDPERLRAYQMSPEEVVQAINKGNVITPAGN
                     VRIQDLMTITALNSVVADIQEFRQLPIRTGAGPTVYLRDVGWVENGSDILTGYALFNG
                     RRAIYIPVTKRSDASTLAVINRVKAALPGFKAVIPEDIDISLEFDQSGYVINTINALS
                     IEIALGSVLTGLMVLIFLRSWRSSLIVLLTIPVALLGSVVALWAMGQTINIMTLGGLA
                     LAVGVLVDASTVAIESIHAHLGKTVSTARAVIDSAVETIAPRFVSMLAVLAVFIPSLF
                     MVGVGRALFVPLALAVGFAMVASYFLSSTFVPIMAVWLLGRRPPEQESEGTFERVKIH
                     YGQWLEVLLPYRRLIAISYVILVGVVILVVGRHLGTELFPTVDTGQFQVRLRAPDGTR
                     IERTEVLAKDVLATIQQEAGTDTVRVTLGYVGTVGSSYPVNLLHLWTSGPHEAILRVA
                     LKRDTGISVEDLKDRLRRKLPERFPGTHYSFEAGDLVSEVLSLGTPTPIEIAITSPNL
                     DLNMTVARQLMANLRNLPTLRDLQFGQPLDYPTVEIDLDRFRAGQLGVTTEDVARSLV
                     AATSSSRFLQRMYWADPSSGIAYQIQIEVPQAQMAGLEDVRNIPVMPSVNGNPWVYVR
                     DVASVMQGTMLGEYDRYNMQRMITITANVAGEDLARAADRVTGAIAALTPLPKGVTVT
                     LRGQVAPMRETMAGLRTGLLLTIVVIVLLLVGTFQSWRNATIVVSSLPAALAGVLLAL
                     LLTETTLNVQSFLGAIMAMGVAVANAILLVSFADAQWRAGCSSGAAAIQGARERLRPI
                     LMTSLAMIVGMIPMALGLGEGGEQTAPLGRAVIGGLLAATVSSLLVLPAIFALIHGRR
                     VATATSLLPDEASTSKPSNVESPLQEELCGPA"
BASE COUNT         1081 a         1370 c         1325 g          899 t
ORIGIN      
        1 gaaacccatc gccaacataa tcaagagaat ccagacgatc gtcggttgag gcatgtccgc
       61 cctctctttc tctgcttaag ttccggccga agtgccatgc aaataagtca atcattatgc
      121 cttcacagaa gccttcttca aagcaccgat tcgagagcag cccgaacctc gactcttgca
      181 cctatttcgg tggaacatca gaatctcgcc gatcgtgcaa caacctttgt ctccggtcgc
      241 aattcgtcgg tgccgtgtac aaccaccgta tcgtgtgacg tcaagtctcc gaaaacttcc
      301 tggagatcgc ccatcacctc tcctcgctgg atcgagaccc attccacggt cccatcaccg
      361 ctgacccgaa tcacaaatat ccgctcagtt gtcgtcacga ccgcgctgcg aggcacgaac
      421 atggtggcgt gtggccggcg cacaggccac tccacttccg caaacatccc cggagccaag
      481 cgtttctttt cattctcgac gtccaattcg accggcattg ttcgagtctt agcgtccact
      541 gaccgagcaa tgcgcctcac cactcctgta aatcgttcgc cgggataggc tgacaccgtg
      601 aaggacactt ccacaccttc ggcgatttca cccgcgttcg tctcaggcac aggaatgacg
      661 agtctcaacc gaccgagctg ttctacgcga agcagcggtt taccttgtcc gggagtcagg
      721 agggcacccg gatgcatctg ccgctcggtg accctgccgt caaagggcgc cgcaattatc
      781 aggtaatcct ccatttcttt gagcgcgacg acacgggcac gaccagcgtc cacggtcttt
      841 tcagcgactt ccaaatcatt caccgcaata actccgggag tttcggccgc tgtgtgcaga
      901 tgctgaagcg tgatttgatc cgcgcgcagc ttcgcctccg cttccgcccg ctgtgcacgg
      961 agttccggtg cgactaaacg ggccagtacc tgcccctgcc ggacgtcatc tccacgatcg
     1021 actccgatcc aattgagcag agcggcaacc ttgggataca gatccacggc caaatacggc
     1081 aagagttcac ccggcagacg cgcggtggtc tgaagttgac gaagttgaac cggcacgacg
     1141 tccacagatt gaggtcgagc tgcgagcgcg ttttgattga gccgctcttg cgagggcgat
     1201 gtggtgctaa aaatggtgag gccactgctg agtgcgagga tcagaacact gccggcgatc
     1261 actttcatgc gggtccgcat agttcctcct ggagcggaga ctcgacattc gacggcttgg
     1321 aagtcgatgc ctcatcgggc aacaacgacg tggctgtcgc aacccggcgt ccatggatga
     1381 gcgcaaagat tgcaggcaac accagcaagc tggagactgt ggcggcgagg agacccccga
     1441 tcacggcacg gccgagcggc gcggtctgct ccccgccttc accgagtccg agcgccatgg
     1501 gaatcatgcc cacaatcatc gccagactgg tcatgagaat cgggcgcagc cgttcgcgcg
     1561 cgccctggat ggcggcagcg ccggaggaac aaccggctcg ccattgtgca tcagcaaacg
     1621 acaccaaaag aatcgcattg gccactgcca ctcccatcgc catgatggcc ccgagaaacg
     1681 attgcacgtt caaggttgtt tcggtcagaa gcagcgctag caacacaccg gcgagggcag
     1741 ccggcaaact ggacaccacg atggtcgcat tgcgccatga ttgaaaggtg ccgaccagta
     1801 ggagcacgat caccacgatc gtgagcagca gaccagtgcg caatccagcc atggtctctc
     1861 gcatgggcgc aacttgaccg cggagggtca ccgttactcc tttggggagt ggagtcaaag
     1921 ccgcaatagc tccggtgaca cgatcggctg cccgagccaa atcttcgccg gccacattcg
     1981 cggtgatggt gatcatccgc tgcatgttgt aacggtcata ttcgccgagc atcgtgcctt
     2041 gcatcacgct ggccacatca cgcacgtaca cccatggatt accgttgacc gacggcatga
     2101 ctggaatatt gcggacgtct tccaagccgg ccatttgcgc ttgcggaact tcgatctgaa
     2161 tctggtaagc gatgcccgac gatggatcag cccaatacat acgctgaaga aaccgactgg
     2221 aagaggtggc cgcgaccaac gaacgtgcca catcttcggt cgtgacgccc agctgcccgg
     2281 ctcggaaccg atccagatca atttcgacgg tcggataatc caatggttgc ccgaattgga
     2341 gatctcgcag cgtcggaagg ttgcgtagat tggccatcag ctgccgggca accgtcatgt
     2401 tgaggtccaa gttcggactc gtgatcgcaa tctcaatcgg ggtaggagtt ccgagactaa
     2461 ggacttcgct cacgagatct cctgcctcaa acgaataatg cgtccctgga aaacgctcgg
     2521 gcagcttacg acgcaggcgg tccttcaggt cctcgaccga aatcccggta tcccgcttca
     2581 aggcgaccct caagatcgcc tcatgaggtc cactggtcca taggtgcagc aggttgacgg
     2641 gataactgga accgacagtc ccgacatatc caagggtaac ccgcactgtg tcggttccgg
     2701 cctcttgctg gatcgtggcg agcacatcct tcgcgagcac ttccgttcgt tcgatccttg
     2761 tcccatcagg cgcccggagc cgcacttgaa attgcccggt atccacagtg gggaagagtt
     2821 cggttcctag atgccggccc acgacaagga tgacgacccc tacgagaatc acatagctga
     2881 tggctataag tcggcggtac ggcagcagca cctccagcca ttggccgtaa tggattttga
     2941 ctctctcaaa tgttccttcc gattcctgtt cgggcggccg tcggccaagc agccagacgg
     3001 ccataatcgg gacgaaggtg ctggagagga aatacgaggc caccatggca aacccgaccg
     3061 ctaaggccag tgggacgaac aaggcacggc caactcctac catgaacaaa gacgggatga
     3121 acacagctaa gacggcgagc atggatacga aacgcggcgc gatcgtttcg acggccgagt
     3181 caattacggc gcgagccgtg gaaacagtct ttccgagatg agcatggata ctctcaatgg
     3241 cgaccgtcga ggcatcaacg agcacaccta cggctaacgc caaccctccc agtgtcatga
     3301 tattgatggt ctggcccatg gcccacaagg cgacgaccga tcccaacagc gcaacgggaa
     3361 tggtcaggag cacgatcagg ctgcttcgcc agcttcgcag gaagatcaag accatcagcc
     3421 ctgtgagcac tgaccccaag gcgatttcga tactgagcgc attgatcgta ttgatgacat
     3481 accccgactg atcgaactcc aacgaaatat cgatatcctc agggatcaca gccttgaatc
     3541 ctggcagcgc ggctttcacc cggttgatga cggccaacgt cgaagcatcc gaccgtttgg
     3601 tgacaggaat gtaaatcgcg cgccgaccat tgaagagcgc atagccggtc aagatgtcgg
     3661 aaccattttc gacccatccg acgtcgcgga gatacacggt gggtccggca cctgttcgga
     3721 tcggaagctg ccgaaactcc tgaatgtccg cgacgacaga attgagcgcc gtgatcgtca
     3781 tcaagtcctg aatccgtaca ttgccggccg gggtgatgac gttgcctttg ttgatcgctt
     3841 ggaccacttc ttccggagac atctgatagg cgcgcaggcg ctcgggatcg atgtggatca
     3901 cgacggtacg ggcgttcccg ccgaaaggcg gcggggcgga gacgccgggc agcgtggcaa
     3961 agagcggacg cacacgaaac aatgccaaat cttgcacttc gccggtgctg cgcgtctcgc
     4021 tgtggaacac cagatatccg acgggcgcgc tgccggcatc gtagcggatg atgtccggag
     4081 gcaatgtgcc gggaggcata aaggcgcgcg atcggtcgat gtaggccact gtctgcgcca
     4141 atgcctggtt catatcggtg cccggataaa agaagagttt gatgagcgcg gcgccttgaa
     4201 tcgatttgga ctcgacgtgt tcaatcccta gaatgtagag gacatggtat tcatagaacg
     4261 agaccagata ggcctccatt tgggatggtg ccattccgcc atacggctgg atgacataaa
     4321 tggcgggcaa tccgaggtcg ggaaagatgt cgatgggcat gctggacaac gcaaggacgg
     4381 cacagagcgc taccgcggcg atgaccacaa tgatcgtcac aggacgacgc aaggcagaaa
     4441 aaagaatcca catgatgaga ctctttccga attcagtgat tccaaggaca gacagagcaa
     4501 gcaatgggcc atgaggcggc tggcggcagg agattgagtc tggccgtcct aattaagaaa
     4561 gacgccgaac cgctggattg ttgaatgttg ttcgcccata gaaggtcgcg gtaagtgaca
     4621 ccggctggaa cacggagaag cagctgcgac cttggttgtc aggagttttg accag
//
LOCUS       DATMTI010000956         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_289786, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000956 DATMTI010000000
VERSION     DATMTI010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289786"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1093
                     /locus_tag="VN039_15110"
     CDS             269..1093
                     /locus_tag="VN039_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47328.1"
                     /translation="MHHSLTILGSGYTAKFLLPLARHRYTHVFATSRDPDRHLTHLSP
                     DQRIRFDLVRPETWQAIPTSTDIVWCFPAVPIGLVRQFAEAASLRTRRLVVLGSTSAY
                     DVCASAEYPPPWVEETAPVDLTKPRVQGEELLRTSYGAIVLRIAGIYGPGRNPVEWIR
                     TGRVNRSRKYVNLIHVEDLVSSCVAALMHADPGGIYNVSDGNPRTWVEICTMVEQRWD
                     IQSPDSQDTDSPGKRVSNKRMCELLKLHGIGQRHRNLFETLELMQRSSLNEATPSR"
     gene            complement(1068..2561)
                     /locus_tag="VN039_15115"
     CDS             complement(1068..2561)
                     /locus_tag="VN039_15115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWV47329.1"
                     /translation="MSEQGFSIQPRPDLPSRDQPTVSDQSAALYSSRSSLVVSGVCAG
                     ILVAALGTILWFSATLPKLQRFEEPDRALDLMVSRMLTVQDSFRRAPEWQQWLTDWTM
                     GSDDEAREQAIQWYRELVETTDDPLSKLRLAILLGESGQETAALAETKRWKNRGTSAL
                     LFERLIEAAYGTQALDRTHEAELQATLAEALPSGWFYDHLAARLARRVGDQELLATVE
                     AQTVRRQDQVLQRISPLIAFELICLVIGSIMLVGIAGLWGQRIDSLRLHLPGVPPPWP
                     GGTAAAVILRGGALGAIMTVSMLSTSSFEHAFHVLAIPLANLPLLALAYVQLLKPAGL
                     TFRNGFGLRIKRENLGRLVCTVLAVVAAGLWGEWVMGRAAEFLNLNDHWTEWFDQDLA
                     WGAPPVMAVSILEYVIFAPIFEELAFRGLLFAMLRRRFAFLPAALISTSVFALAHGYS
                     PIGFLSVFWSGFLWAWIYERTGSLIPGMVAHATNNLLVCLTVTALLR"
     gene            2667..3437
                     /locus_tag="VN039_15120"
     CDS             2667..3437
                     /locus_tag="VN039_15120"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWV47330.1"
                     /translation="MLRLRDDQWERIREHFPEEHIPDSRPGRKPVPTRAVLDAVLWIL
                     NTGAQWHFLPQCYPNYKTVHRRFQQWCQQEVLREVLTDLANTLRDEGAIDERERVIDA
                     TCAAAKGGGNAVGLTKRGKGVKILAIVDRHGLPLSVSTHAANHHEVTLVQLSFDFYML
                     EAKPEHLIGDRAYDSDGLDEDLQQDGVNMIAPHRSTRKLKTQDGRHLRRYQRRWLVER
                     FFAWLQWKRRLLVRWEYYATNFLGFIQLACITMLLKQF"
BASE COUNT          826 a         1049 c          975 g          736 t
ORIGIN      
        1 gtatagagca cggctgttat cccttgaaat cctccatctc cgccgcctcc ttcgggacat
       61 ccggccaaga gcagcatgaa cggaagcgcg agccatgacg gtactcgtat cttcatgtgg
      121 tgtcctcttg ttcgtctcca atgtgaaatg tctattcctc gtataacacg aggaaatacg
      181 tacaccaact aaaatgactt ttttcgttgg actctgatcg gttctcgctg gcacactgct
      241 cacacgtcag cttgggatac gactgatctt gcatcattca ctcaccattc tcggatccgg
      301 ctataccgcc aagtttctcc tgcccctcgc ccgacaccgc tacactcatg tcttcgccac
      361 cagtcgagat ccggatcggc acctcacaca tctgagtcct gaccaacgga tacgattcga
      421 cctggtacgg cccgagacat ggcaggcgat tcctacatca accgatattg tgtggtgctt
      481 tcccgcagta ccgatcgggt tggttcggca gttcgccgag gccgcatctc tacgcacgcg
      541 ccgcctggtg gtactcggca gtacctccgc atacgacgtc tgtgcctcag ccgagtaccc
      601 tccaccctgg gttgaggaaa ccgcgccggt tgacctgaca aagcctcgtg tacaaggcga
      661 ggaactattg cgaacttcct atggcgccat cgtcttgcga attgcgggga tctatggtcc
      721 cggtcgcaat cctgtcgagt ggatcagaac aggccgagtg aatcgatcac gcaaatatgt
      781 caacctgatt catgttgagg atttggtttc gtcctgcgtt gcagccctca tgcacgctga
      841 ccccggcgga atctacaacg tcagcgatgg aaatccgagg acctgggtgg agatctgcac
      901 gatggtcgag caacgatggg atattcaatc ccctgactct caggataccg actcaccagg
      961 aaagcgtgtg tcaaacaaac ggatgtgcga gttgctgaaa ttgcatggaa taggtcagcg
     1021 ccataggaat ctattcgaga cgcttgagtt gatgcagcgg agttcgctca acgaagcaac
     1081 gccgtcacgg tgaggcacac gagcaagttg ttcgtcgcat gggcgaccat accgggaatc
     1141 agacttcctg tccgttcata gatccatgcc cagaggaatc cgctccaaaa aacgctgagg
     1201 aatccaatcg ggctatagcc gtgggccaga gcaaagacac tggtgctgat cagggcggca
     1261 ggaagaaatg caaaccggcg gcgaagcatc gcgaacagta atccacgaaa cgccagctcc
     1321 tcgaagatcg gtgcgaaaat gacgtactcg aggatactca cggccatgac gggcggagcc
     1381 ccccacgcaa gatcctgatc aaaccattcc gtccagtggt cattcaaatt caggaactcg
     1441 gcggcgcgtc ccatcaccca ttcaccccag agtccggctg cgaccaccgc aaggacggtg
     1501 cataccaggc gtccaaggtt ctctcgcttg atccttagac cgaagccgtt ccgaaaagtc
     1561 aggccggccg gcttgagcaa ttgaacatac gcgagggcaa gcagcggtag gttggccaag
     1621 ggaatggcca agacatgaaa cgcatgctca aatgacgacg tggacagcat acttacagtc
     1681 atgatggctc ccaaggcacc accgcggagg atgactgcag ccgcagttcc tccaggccaa
     1741 ggaggcggaa cgcctggtaa atggaggcgc agactgtcta tccgctgtcc ccacagtccg
     1801 gcaatgccca caagcatgat cgaccctatc accaggcaaa tcaattcgaa cgcgatcaac
     1861 gggctgatcc gttgcagaac ctgatcctgt ctgcgtaccg tctgcgcttc aaccgtcgcc
     1921 aacagctcct gatcgcccac gcgccgtgcc aaccgagcgg caaggtgatc atagaaccat
     1981 ccgctcggca atgcctccgc cagcgtggcc tgtaattctg cttcgtgcgt ccgatcaagc
     2041 gcctgggtcc catatgcagc ttcgatcagt cgttcgaata gcaatgcaga cgtaccacgg
     2101 tttttccagc gcttggtttc agcgagcgcc gctgtttctt gtccggactc cccgagtagg
     2161 atagccagcc gcagcttgga gagagggtca tcagtcgtct caaccagttc ccgataccac
     2221 tggatagctt gttccctggc ttcatcatca ctgcccatcg tccagtcagt cagccattgc
     2281 tgccattccg gcgctcgacg aaaactgtct tgtacggtca acatacggct gaccatcaga
     2341 tcaagcgcgc gatccggttc ctcaaatcgc tggagttttg gcagggtcgc cgaaaaccac
     2401 agaatggtgc cgagcgcagc aacgaggatg ccggcgcata ccccgcttac aaccagagac
     2461 gatcgagagc tatacagagc ggccgattga tcggaaactg tcggctggtc gcgcgacgga
     2521 agatccggtc gcggttggat tgaaaaaccc tgttcgctca tgagctgccg actataccat
     2581 tggccgtcac ctaaccaaat cccctcaaag tagaacctat ctcaaaatag ttgacggcct
     2641 gaattccaat ccgtataagt cggggcatgc tgcggctgcg ggacgatcaa tgggagcgga
     2701 ttcgcgaaca ctttcccgaa gagcacatcc cggattcccg tcctgggcgg aagcccgtcc
     2761 ccacccgggc ggtgttggac gcggtgctct ggattctgaa taccggggcc cagtggcact
     2821 tcctgccgca gtgctatccg aattacaaaa cggtccatcg tcggttccag cagtggtgtc
     2881 aacaagaggt gctgcgggag gtgctgaccg acttggccaa cacactgcgc gacgaaggag
     2941 ccattgatga acgcgagcgt gtcatcgacg cgacgtgtgc cgcggccaag ggcggcggga
     3001 acgccgtcgg cctcacgaaa cgtggcaagg gcgtgaaaat cctggcaatt gttgatcggc
     3061 atgggttgcc actctcggtc agtacgcacg cggcaaatca tcatgaagtc accttggtgc
     3121 aactcagttt cgacttctac atgctggagg ccaagccgga gcatctcatt ggcgatcgcg
     3181 cctatgacag cgatgggctc gatgaggatc tccagcaaga cggcgtcaac atgatcgcac
     3241 cgcatcgctc cactcgaaaa ctcaagactc aagacgggcg ccacttacgc cgttatcaac
     3301 gccgctggct cgtcgaacgg ttctttgcct ggctgcaatg gaaacggcgg ttgctggttc
     3361 gctgggagta ctatgcgact aacttccttg gctttatcca gctcgcctgc atcacgatgc
     3421 tcctcaaaca attttgagat aggttctagg gcgtgtcgtc agttaaggga ttcacaaagc
     3481 tgagcatatg tgattcatag aggggtagcc tgaacggagg ggctacccga tggcacgacg
     3541 gtatgcactg cgcgacgatc aatgggaacg catcaaaggg tttcta
//
LOCUS       DATMTI010000957         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_843118, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000957 DATMTI010000000
VERSION     DATMTI010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843118"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VN039_15125"
     CDS             <1..646
                     /locus_tag="VN039_15125"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV47331.1"
                     /translation="SSPFPRIVLMGEIVMLLMSSEAAKTAQDQEWTQLIALTAQGDQT
                     ALATFYDRTSPQVFGLVLKILNNREAAEEVTLDVYTQVWRQAHTYDRARGAPGAWLMT
                     LARTRAIDRFRAGAAEHGRIESLDAVELFVSADDTPEQDVEGQERRRYVQQALEVLTA
                     EQRQAIALAYFYGLSQSEIAEKLQLPLGTVKTRIRLGMMKLREALAPYEAGLTP"
     gene            643..1548
                     /locus_tag="VN039_15130"
     CDS             643..1548
                     /locus_tag="VN039_15130"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV47332.1"
                     /translation="MTEHKLPEELEEQAVFHALGILEPDDRRVFMVRLQEESDLLRQS
                     VIAYQATTDALAAVVVPITPPSALRERIVNQVALEAGREAEQFELAISTVALASLPVK
                     PRDAVRERLLSRIEGHSDIRLDATDSVRVLNETPALPDAGGRNESESGSLFGRPRSFW
                     TAISNFLRTILIRSMTSERVRYWKAKIALQQPTKGLTFIRASEGTWRGIAPGVMVKLL
                     SFDPTSGRTTTVLRFAPGTSYAPHRHTAVEELYVLEGGCSIAGREMTVGDYHRAEAGT
                     VHHDTSTDEGCLLLVISSPQNEMLR"
BASE COUNT          411 a          505 c          549 g          382 t
ORIGIN      
        1 atcttcccca ttccctcgta tagttttaat gggggagatt gttatgttgc tcatgtcgtc
       61 agaagcggcc aaaacagctc aagaccaaga atggacacag ctgattgctc tgacggcaca
      121 gggagatcag acagcgctcg ccacgtttta cgatcgtacg agcccgcagg tctttgggct
      181 tgtcctcaaa attcttaata atcgcgaggc cgctgaagaa gtcacattgg acgtctacac
      241 gcaggtgtgg cgtcaggccc atacctatga ccgggcccga ggggcaccgg gggcttggtt
      301 gatgactttg gcccgtacaa gggcgatcga ccggtttcgc gccggggcgg ccgaacacgg
      361 gcgcatcgaa tcgttggatg ccgtcgaact attcgtcagc gccgacgaca ccccggagca
      421 ggatgtcgaa ggccaggagc gccgacggta tgtccaacag gcgttggagg tgctgacggc
      481 ggaacagcgg caagcaatcg cactggccta tttctatggc ttaagtcaaa gtgaaatcgc
      541 ggaaaaactg cagctgcccc tcggtacggt gaagacgaga attagattgg gtatgatgaa
      601 attgcgcgaa gcgctcgcgc cttatgaggc gggtttaacg ccgtgaccga acacaaactg
      661 ccggaagaat tggaagaaca ggcggttttc catgccctcg ggatattgga gccggacgat
      721 cgccgggtat ttatggtgag actgcaagaa gaatccgatc tcttacgcca atcagtgata
      781 gcgtatcaag cgacaacgga tgccttggct gcagtcgtgg tgcctatcac gcctccatcg
      841 gcccttcgag agcggatcgt gaaccaggtg gcgttggagg ccggacgaga agctgaacag
      901 tttgagctcg ccataagtac agtggccttg gcgtcgcttc cggtaaaacc tcgcgatgca
      961 gtgcgggaac ggctcctctc acggatcgaa gggcattcgg atatccgact tgatgccaca
     1021 gactcggtgc gggtcctcaa cgaaacgcct gccctaccgg atgcgggggg gaggaacgaa
     1081 agtgagagtg gctccctctt cggtcggcca cgatctttct ggacggctat ttcaaatttc
     1141 ctgcggacca ttctcattcg ttccatgacc tcggagcgag tcaggtattg gaaggccaag
     1201 attgcgcttc aacagccgac gaaggggctt accttcatca gagcgtcgga aggaacctgg
     1261 cggggaatcg ctccgggcgt gatggtcaag ttgctttcct ttgatccgac ttccggaagg
     1321 accaccacgg tcctccggtt tgcacccggc accagctacg caccgcatcg tcataccgca
     1381 gtggaagaac tgtatgtcct cgaaggcgga tgcagtatcg ccggacgcga gatgacggtt
     1441 ggagactatc atcgcgccga agcagggaca gtgcatcatg atacatcgac ggacgaaggg
     1501 tgtctgctgc ttgtgatctc ttcaccgcaa aacgagatgc tgcggtagcg ggaaaatttg
     1561 gtaacgctcg ccgtaagacg ctgggcgctc acgggacacg gcccgttcac catctcgcgg
     1621 gcgtccacag acgtgccatg ccttattcgg catggcgtgg acctcgcgtc cgtccgataa
     1681 aaactcaaga cactgccggc tcaccaactc gccggcgcgc acaagcatgg cgctctttat
     1741 tcatcgcgcc gtgcgcctca ttattcttcg cgtcgcggac cacgtgaatc caatcatcgc
     1801 cgaattgccg taagacaatt agtgcgagca tcgccgattg atttttg
//
LOCUS       DATMTI010000958         1974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_545639, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000958 DATMTI010000000
VERSION     DATMTI010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545639"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /gene="ndhC"
                     /locus_tag="VN039_15135"
     CDS             complement(<1..352)
                     /gene="ndhC"
                     /locus_tag="VN039_15135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWV47333.1"
                     /translation="MTGTEVLLEYLTKFFPVFLFILVTLTFGVVTLLVSYFVQPRYPE
                     PEKLSTYECGAEPFSDARMPFPVRYYIFAMLFVIFDIEVIFLYPWAVVFTKIGVIGLI
                     EMMIFIGLFIVAYVY"
     gene            complement(540..734)
                     /locus_tag="VN039_15140"
     CDS             complement(540..734)
                     /locus_tag="VN039_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47334.1"
                     /translation="MNVDEKKDQHLSGSFRQMERIDTQIHRRRKVKAFSPLESEWEAS
                     QAGRSASGDDEQSEEISVDA"
     gene            902..>1974
                     /locus_tag="VN039_15145"
     CDS             902..>1974
                     /locus_tag="VN039_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47335.1"
                     /translation="MKTLASLVLVLCSGIGISLDEALAQVSEIDSIRFSEAALTFSSG
                     AIQKTSPIDGTVNLVTGDNQSTGNRMLLGKQDSLYLKLNNPTDVAVGDLFTVYRRVRK
                     VFHPVTKEYLGFVMNRSAVVKVTAADHALTTVEAVLSYGPISPGDPVMRFVAPAPDIE
                     ASSATNISDLEGMIVELQADRTMTLVSQSNVVYLDRGKEDGLKVGDLLNIHRHSAGLP
                     PRKIGQLKVLSLESHTATAKIFKANTRVIKGDRFKLVGYSTPTIQSSVGASPESPTAQ
                     ARQTAPADPVSSKLKIQDASGQSRLNLGDLAKFLHFDSGDAAIKPENYKVLDQLIEYL
                     RTSGDMRMIRIEGHTDNVEIGPSL"
BASE COUNT          516 a          517 c          483 g          458 t
ORIGIN      
        1 catacacgta ggccacaatg aacagcccta taaaaatcat catttcaatc agcccaatca
       61 ccccgatctt ggtgaatacg acagcccacg gatagaggaa gatcacttca atatcaaaga
      121 tgacgaacaa catcgcaaaa atgtaatatc tgacgggaaa cggcatacgg gcgtcggaaa
      181 atggttcggc gccacattcg taggtcgata atttttctgg ttccggatac ctgggctgca
      241 caaaatagct aacgagcaag gtcacgaccc caaaggtcaa tgttaccaag ataaagagaa
      301 agaccgggaa aaacttcgtc agatattcaa gaagaacttc ggttccggtc atttagtttt
      361 ctctcgcaag ctattgacct ggaaagggca ttatagatcg gatacatgaa atcttagtgc
      421 cccggtcttg gggatagcaa gcgaacaaag gacctaattc cggaagatcc gaaagcccta
      481 gcggcctgag aacgaaaggt gggtaggttg aatggttagg ttcttattac aattcacgat
      541 caggcatcaa cgctgatctc ttcgctttgt tcatcatccc cgctggcgga tcgtccggcc
      601 tgcgaagcct cccattccga ttcgagagga gaaaacgcct tgaccttccg tcgccgatgg
      661 atctgtgtat cgattcgttc catctggcgg aagctcccgg agagatgttg atcttttttt
      721 tcgtcaacat tcatcggtca gtcatgatcc ctgaaatcat tattagggga tggcaaggtc
      781 cgacgttacc actggaaaga ccggcccgtc aaattccgac caattcagct caaattcttg
      841 acaacacaac gccttttgct atgttttttc aagcaacact agcctcggaa ggaatcttcc
      901 tatgaaaacc ttggcgtccc tcgtcttagt cctgtgctca ggaatcggca tctcactcga
      961 cgaagcacta gcacaggtgt cggagattga ttccattcgg ttttcagaag ctgcgctcac
     1021 cttttccagc ggtgccatcc agaaaacatc cccaatcgac ggaaccgtaa acttagtaac
     1081 aggtgataat cagtcaacag gcaatcgcat gcttcttggt aagcaagatt cgctctatct
     1141 caagctcaac aaccccactg atgtggcagt tggtgatctt tttaccgtct accggcgggt
     1201 tcggaaggtg tttcatcctg tgaccaagga atacctcggg tttgtcatga atcgttcggc
     1261 ggtcgtgaag gtaactgccg ccgaccatgc tctcacgaca gtagaggccg tgctcagtta
     1321 cggaccgatt tctcctgggg accctgttat gcgctttgtg gctccggctc cggacatcga
     1381 ggcgagctcc gcaacaaaca tttctgacct cgaaggcatg atcgttgagc ttcaagccga
     1441 ccggacgatg acgctggttt cacagtcaaa cgttgtgtat ctggatcgag ggaaggagga
     1501 cgggctgaag gtcggtgatc tcctcaatat tcaccgtcac agtgcggggc ttccgccacg
     1561 aaagatcggc caacttaagg tgctgtcgtt ggaatcccac accgcaaccg ccaaaatctt
     1621 caaggccaac acccgcgtca tcaaaggaga ccggttcaag ctcgtcggat attcgacacc
     1681 cactattcag tcgtcggtag gagcttcccc ggaatcgccc acggctcagg ccagacaaac
     1741 ggccccggct gatccagtat ccagcaagct gaagatacag gatgcatcgg gacaaagtcg
     1801 tctcaacctc ggggatctag cgaagttcct gcactttgac tcaggggatg ctgccattaa
     1861 acctgaaaat tacaaagtgc tcgatcagtt gattgaatac ctgcgcacca gcggcgacat
     1921 gaggatgatc cgaatcgaag gtcatacgga taacgtggag atcggccctt cgct
//
LOCUS       DATMTI010000959         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_206612, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000959 DATMTI010000000
VERSION     DATMTI010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206612"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..605
                     /locus_tag="VN039_15150"
     CDS             198..605
                     /locus_tag="VN039_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWV47336.1"
                     /translation="MEHRLATFFGRSPVGPKGGKEEMTAAEWTPLVDITEDEKEYLIK
                     AELPEVNKEDVKLAVHDDVLTITGERKYEKEETNKKFHRIERAYGSFTRNFMLPEDAD
                     ETKVAADFKDGVLKVHVPKSQKAKPQSTEIRIT"
     gene            742..1401
                     /locus_tag="VN039_15155"
     CDS             742..1401
                     /locus_tag="VN039_15155"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HWV47337.1"
                     /translation="MSPDSTMESAQNRILAGLETVHKHWGYFLASGIAFLALGTIALA
                     YTVLTTLASVFVFGVVLLIGGIFQTGHAFKMSTSKWSGFLLDLLIGVLYVITGLLMAV
                     HPLAGAQSLTLLLASFFLVVGVFRTMGAVILRPPSWGWLLVSGIITFLLGILVWIQWP
                     ESALWLIGTLVAIDMIFNGAWLAMLAVNIRRMTPQEIQERATDALPRALFEQQPTRQS
                     G"
BASE COUNT          376 a          427 c          478 g          354 t
ORIGIN      
        1 taccgacggg gtgtgcagct agatttcatt cagcctggca aaccatatga gaacgcctgg
       61 gaagagttct tataacttcc gcacacaatt tccatctatt gcacagacag tttcataatg
      121 gaggatttta catgaacgta cttacccgtt gagacctatt taagacacat tggcatccga
      181 tcaaggaact ggaggagatg gagcatcggc tggcaacatt cttcggtcgt tccccggtcg
      241 gcccgaaagg cggcaaggag gaaatgaccg ccgccgaatg gacgccgctc gtggacatca
      301 cggaagacga aaaggaatac ctcattaagg ccgagctgcc cgaggtcaac aaggaggacg
      361 tgaaactcgc ggtccatgac gatgtcctca cgattacagg cgagcggaag tacgaaaagg
      421 aagaaacgaa caaaaagttt catcgcatcg agcgggccta tggaagcttt acgcgcaact
      481 tcatgcttcc ggaagatgcc gacgaaacca aagtcgccgc cgacttcaaa gacggtgtcc
      541 tgaaggtcca cgttcccaaa tcccagaaag ccaaacctca gagcaccgag atcagaatta
      601 catgaggcta cggggaggga accgacgggg gaagatgact cgctgtctgt cggaccatcg
      661 aacgtcgtcg gttctcatgc accgggtgtc tgcggcctca tgatatgagg ccaggactct
      721 cgtaagtgag aggagatgca tatgagccct gactcgacca tggagagtgc tcaaaatcgg
      781 attcttgccg gccttgagac agtgcacaag cattggggat attttcttgc ttcgggaatc
      841 gccttcctgg cgcttggcac gatcgctctg gcctatacgg ttctcacgac gctggcctcc
      901 gtctttgtgt ttggagtggt gctcctgatc ggcgggatat tccagaccgg tcatgctttt
      961 aagatgtcca cgtccaagtg gagcggattc ctcttggacc tcttgatcgg cgtgctctat
     1021 gtcatcaccg gtctgctgat ggcggtgcat cctttggcag gagcccaatc gctgactctg
     1081 ctgctcgcct cgttttttct ggtcgtggga gtgtttcgga ccatgggggc tgtaatcctc
     1141 cgaccgccca gttggggatg gctgctcgta agcgggatta tcacgtttct gctcggcatt
     1201 ctcgtgtgga tccaatggcc ggaatcagcc ctgtggctga tcgggacgct cgtcgcgatc
     1261 gacatgatct tcaacggcgc ctggctggcc atgctcgcag tgaatatccg tagaatgacg
     1321 ccacaggaga ttcaagagcg cgcgacggac gctctgccga gagcgctctt cgaacaacag
     1381 ccgactcgac aatccggatg agactgagag gcactgggga ggctggtaca tcttaacccg
     1441 gtacggaagc gcgtccggct catgcgcgcg gaaggaggac gatcatgcgt tcgaccctgt
     1501 gtagacttgc gatggcggtc acaatcggaa tgatgcctat aggagccgga gccgaatcaa
     1561 caccgtcgaa agctcctgag ggctatctcc ttgtcgacga ggaagtgtgg cttgtcatcg
     1621 atgacgagtc ggaag
//
LOCUS       DATMTI010000960         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_206614, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000960 DATMTI010000000
VERSION     DATMTI010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206614"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..303)
                     /locus_tag="VN039_15160"
     CDS             complement(148..303)
                     /locus_tag="VN039_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47338.1"
                     /translation="MVKQPQTAPHPDSVVTPPVIDPKMAIDPEAKTEEERHPTLPPEQ
                     AEPPKQQ"
     gene            596..1552
                     /locus_tag="VN039_15165"
     CDS             596..1552
                     /locus_tag="VN039_15165"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWV47339.1"
                     /translation="MSLLIICFLLTYLTNEPAAAATQYQPAELRILTYNLLHDGPSSG
                     FFENGTHLEERLDMTIQELRRLQPDIIAFQEASDSRRHGNVPQRIADALGYHMVFAPA
                     TERIFGIGRLDRLIISILGFKEGPAILSRYPIVDSEVYDLPRCRYRLDPRILLRAEID
                     IPAGPVQVFSAHTSKGDECQLQRVGELFQAHRGTGQAILMGDLNTGEQSSVLAGWQKE
                     PGFIDVFRLANPGISGGTVWQDIHVERSTADRRVDFIFLLGGRKVEGPIVRSSRLAFD
                     RPGRLPSGAVLWPSDHRGVLADIELAPVDRLRMGRLSDTSTR"
     gene            complement(1651..>2598)
                     /locus_tag="VN039_15170"
     CDS             complement(1651..>2598)
                     /locus_tag="VN039_15170"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWV47340.1"
                     /translation="KDLTVNQIPMNATIDASNRTTYTPAILQMEAGERQFWRVSNSTA
                     DSILDLHVQFDGVPQTIEIVAIDGVPVNSQDGAQPGSLIPVTHFRLPPASRVEFIVNA
                     PLPNVKVAQLVTQNTNTGIDGDSAPNRPLFNIQVGVDGNAALAEDDRVGPSVAFAPHL
                     NRFAGLTAAPLATRRLVFFKENRPTQFFMNVEGQPEKVFDPNAGPAMTATQGTVEEWT
                     VENRTRENHEFHLHQLHFLVEGQSNFEINGNEQVPGISGQFLDTIEVPHWDGDLTHPF
                     PRVTLRIDFRGQNIGDFVFHCHILVHEDLGMMNIVRVQP"
BASE COUNT          523 a          734 c          745 g          596 t
ORIGIN      
        1 tcactcccac atctcactgt gccctcgacg gtccattgtt ttcgccggat tgcacggaag
       61 aagagctgca cgcggcagcc ggctactatc aggtccttga tatctttctt ggcatcagga
      121 atgatgggca ttgccatccc tcgaccgtta ttgttgtttc ggcggttcgg cctgttcagg
      181 cggaagtgtc ggatgccgct cctcttctgt ttttgcctcc ggatcaatgg ccattttggg
      241 gtcgatcaca gggggcgtca cgacagaatc cgggtgagga gccgtctggg gctgtttcac
      301 catgccagga tcacgagtac taggaggact gtccggaact gatttctcgg tcgagggatc
      361 ttgtggcgga ggcgattggg acggcggagt gatatcggcc cccagcacag gtgaaccact
      421 tacagtgagt agtataatga agagccatcg ccgcatgatt taacgatatc ggatgacatg
      481 cgttctgcct actcggagat acccctgatg tctataatag gtaggttctt cgtgaaacgc
      541 tcacttgtta gatccttatg accattttgc ttgtcctaca atgcgatcga tcctcatgtc
      601 gttgttgatc atttgctttc tgctgaccta cctgacgaat gaaccagcgg cagcagcaac
      661 tcaataccaa cctgccgaac tccgtatctt gacttacaac ctcctgcacg acgggccatc
      721 gtcgggattc ttcgagaacg gcacgcacct cgaagagcgg ctcgacatga ccattcaaga
      781 actgcggcgc ttacagcctg atatcattgc ctttcaggaa gcgtcggaca gccggaggca
      841 tggcaacgta ccgcaacgga tcgccgatgc gcttgggtat cacatggtgt tcgcccccgc
      901 gaccgagcgt atcttcggca tcggccgctt ggatcgactg atcatctcga tcttagggtt
      961 caaagagggg cccgccatct tgagccggta tccgatcgtt gactcggagg tctatgactt
     1021 gccccgctgc cgatatcggc tcgaccctcg cattctgctt cgtgccgaga tcgacatacc
     1081 cgccgggccg gtccaggtct tttcggccca tacctcgaaa ggcgacgaat gccaactaca
     1141 gcgagtcgga gaactgttcc aagcgcatcg aggaacggga caagccatcc tgatgggcga
     1201 cttgaatacc ggcgagcaat cttcggtcct cgccggatgg cagaaggaac cgggattcat
     1261 cgatgtattt cggctcgcca acccaggaat atccggcggg accgtctggc aagacatcca
     1321 cgtcgaacgg tccaccgccg atcgccgcgt cgattttatt ttcttgctgg gtgggagaaa
     1381 ggtcgaaggt ccgatcgtgc gttcaagtcg cctggcattt gaccggccgg gtcgtcttcc
     1441 gagcggcgcc gtgctatggc cgtctgatca tcgcggcgtg ttagcagata ttgagttggc
     1501 tcctgtcgat aggttacgga tgggccgtct atcagatact agcactcgtt aaagagctgg
     1561 cccaggacgc acgattcaag atccgacccc tactctttcc cgttctctgt tccgttcctg
     1621 ttcagaaacc ggttagttcc ccggagggga ttacggctgg acgcggacga tgttcatcat
     1681 gcccaaatcc tcatggacca ggatgtggca atgaaacacg aaatcaccga tgttctgtcc
     1741 acgaaagtcg atccgaagtg tgacacgggg aaacggatgc gtcagatctc catcccagtg
     1801 tggaacttcg atcgtgtcga ggaattgccc gctgatcccc ggcacctgct cgttgccgtt
     1861 gatttcaaag ttgctctgtc cttccaccag gaaatgaagc tgatggaggt gaaattcgtg
     1921 gttctcccgc gtacgatttt ccaccgtcca ctcctcgaca gttccctggg tcgcggtcat
     1981 ggcggggccg gcgttcggat cgaacacctt ctccggttgc ccttccacat tcataaagaa
     2041 ctgggtcggc cggttctcct tgaaaaacac cagccgccgt gtggcaaggg gcgcagcggt
     2101 caggccggca aaccgattca ggtgaggggc gaacgccacg gaggggccga cgcggtcatc
     2161 ctcggcaagt gcggcgttcc catcaacacc cacctgaatg ttgaagaggg gccgattggg
     2221 cgcactgtca ccgtcgattc ccgtgttggt attctgggtc accaattgtg cgaccttcac
     2281 attaggcagg ggggcattca cgataaattc tacccgggac gcaggcggca gacggaagtg
     2341 cgtcaccgga atcagactcc caggctgagc gccgtcctgg gagttgaccg gcaccccgtc
     2401 aatggccacg atctcgatgg tctgcggtac gccgtcgaac tgcacgtgca gatcgagaat
     2461 gctgtccgcg gtggagttgg atacacgcca aaactgtcgc tcccccgctt ccatctgaag
     2521 aatggcgggg gtataggtcg tgcgattgct cgcgtcaatg gtggcgttca tggggatctg
     2581 gttgaccgtg agatcctt
//
LOCUS       DATMTI010000961         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_119937, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000961 DATMTI010000000
VERSION     DATMTI010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119937"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VN039_15175"
     CDS             <1..257
                     /locus_tag="VN039_15175"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HWV47341.1"
                     /translation="TAHDPILKVESGSGFEVEFEFDALMAPGFYFLNVGVVEQEGKGI
                     NYLNRMLDVAMFRVQDDLARISTGQVNLNVAVRYRNLQEI"
     gene            295..2301
                     /locus_tag="VN039_15180"
     CDS             295..2301
                     /locus_tag="VN039_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47342.1"
                     /translation="MLGMHRSGTSATAGVLRLLGFDLGQHLLDPAEDNVAGYWENASV
                     VQANEALLAAAGRTWDDFRYFGRSELLQSAKKTGVDFISIVTQEFGKNRLVAVKDPRM
                     CLTYEVWRKAFTARAYAVSVLVVCRHPDAVVRSLEKRDHMPPVIGYTLYIRYSLEAVS
                     AAMSADSYAALDYTDVLSDWRSVFRDVLGKLDLVEIMGDVSNHSPIDRFVDANMSHSG
                     EVASETEDDDVQGLRTIAISAYEMARECEKWSRAALDDLISRFERCLLGQSPSNAARF
                     DTHLRWCARHEESVKGFRDMLRGNVDQLHRVEVGLQEAGSLALERLARIEEQQKQLEK
                     LEISEVLLKQGRLELDAANTARDDCQSRLTELEAGLKLASRLSIERLGLLEAEGRRIA
                     RLESRVEELSVEATSNLELAEAKDLECRRLTEALECVTRLCNDRLEQIHEQESTLQSY
                     DESLNSVTQQALDRLAEMEQLSKRLQNEQATLAATQEAFEVASGLAIERLQLLSKQDE
                     HVGALENRIEELSVAATSNLELAEAKDLESRRLTEALERVTRLCNDRLEQINDQESSL
                     KMYHDKLNATVQRSTEKVATLEKRLHRLDQEKLVLESDRDAALAKVGRLSENYERLVD
                     EHERTKRSVKRLHDKALRFASQAIEARRSLIARLRLRNARRKGS"
     gene            2305..>3220
                     /locus_tag="VN039_15185"
     CDS             2305..>3220
                     /locus_tag="VN039_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47343.1"
                     /translation="MYHYNLDSLIFRDRLAFGWGWFVDLEARVFPVRVVLQHADDSVS
                     TILCLRSGTRTDVADAFPDVNHAAASGFMIQGRLDSDSPVSTARLVLAGVDGNVGGTE
                     IELGALASMLQQDVRATVSRGIRILRRRSFSANLGIVAKRLRIHVREALAARRSISGR
                     RNAILILDHDLGGGSNAFRRQMIGRLRQTSPVYLITPRVQTLDYYLTLYDGVVESGQV
                     YERLADLLQFLDGESWASIVINELVSYPDPMAMVKWLSARASVVPASTDFVLYLHDFY
                     SVCPQWTLVGRRGVYCGVPDFEECTRCIK"
BASE COUNT          751 a          745 c          959 g          765 t
ORIGIN      
        1 ccactgcgca cgatccaata ttgaaagttg agtctggatc tggtttcgag gtcgagttcg
       61 aatttgacgc gctgatggct ccagggttct atttcctcaa cgttggggta gttgagcaag
      121 agggtaaggg tattaactac cttaatcgta tgctcgatgt agccatgttt cgcgtgcaag
      181 atgacttggc aagaatttcc accggacaag tcaacttgaa tgtcgcggtt cgataccgaa
      241 atctacaaga aatatagacc atcaaaggcg acgtccatga agagatcgct tctcgtgttg
      301 ggtatgcacc gaagtgggac gtcggcaact gctggtgttc tgcgtctgct cgggttcgat
      361 ctggggcagc accttcttga ccctgctgaa gataatgtag caggctattg ggaaaatgcc
      421 tccgttgtgc aagcgaacga agcgttattg gcggctgcgg gccgtacttg ggatgatttt
      481 agatatttcg ggcgatcaga gttactccag tctgctaaaa aaacaggcgt cgacttcata
      541 tccatagtta cccaagagtt tggaaaaaat aggcttgttg ctgtaaagga tccgcgcatg
      601 tgcctcacat atgaggtatg gcgaaaagcc ttcacagcga gagcttatgc ggtgtccgtt
      661 ctggtcgtat gtcggcatcc ggatgcagtg gttcgctcat tggaaaagcg cgaccatatg
      721 ccgccggtta ttggttacac cctatatatc cggtactcgc tcgaggcggt gagcgctgct
      781 atgagcgcgg attcgtacgc agcattagac tacacggatg ttctatcgga ctggcgctca
      841 gttttcaggg atgttctggg taagttggat ttggtcgaga ttatggggga cgtgtcaaat
      901 cactctccta ttgaccggtt tgttgatgca aatatgtctc atagcggtga ggtggcgtca
      961 gaaaccgagg atgacgatgt tcaggggctt cggaccatag ccatatccgc ctacgagatg
     1021 gcgcgtgaat gtgagaaatg gagtcgcgcg gctttagatg acttgattag tcgttttgag
     1081 cgatgtcttc ttggccagag tccaagcaac gccgcacgtt tcgacaccca tcttcggtgg
     1141 tgcgcaagac acgaagagtc cgtgaaaggg tttcgagaca tgttacgggg aaatgttgac
     1201 cagttacatc gagtggaagt cgggttgcag gaagcaggta gtttggcgct tgaacgactc
     1261 gcgcgcatcg aggaacaaca aaagcaatta gagaaacttg agatatctga ggtgttgctg
     1321 aagcaaggtc gtctggaatt ggacgccgcc aacaccgcgc gggatgactg tcaatcacgc
     1381 ctaactgagc tggaggcagg cctcaaacta gcgagcaggc tttcgataga gcgtttggga
     1441 ctgctagaag cggagggtag gcgaatagcc cggctcgaaa gcagagttga ggaactgagc
     1501 gttgaagcga ccagcaactt agagttggct gaagcgaaag acttggagtg ccgccgtctg
     1561 acggaagctc tggagtgcgt gacgcgcctt tgtaacgacc gcctcgaaca gatccacgag
     1621 caggagtcga cccttcaatc ctatgacgaa tcacttaata gtgtgacgca acaggcactg
     1681 gatcgtttgg ctgaaatgga gcaactctct aaacggcttc agaacgagca ggctaccctc
     1741 gcggcgacgc aggaggcgtt cgaagtggct agtgggcttg cgattgagag actgcaactt
     1801 ctaagcaagc aggatgagca tgtgggggcg ctcgaaaata gaattgagga actgagcgtt
     1861 gcggcgacca gcaacttaga gttggctgaa gcgaaagact tggagagccg ccgtctgacg
     1921 gaagctctgg agcgcgtgac gcgcctttgt aacgaccgcc tcgaacagat caacgatcaa
     1981 gaatcatcgc tgaagatgta tcatgacaag ctgaacgcca ccgtgcaaag gtctacggag
     2041 aaggtagcaa cgctagaaaa gaggcttcat aggctggatc aagagaaatt ggtccttgag
     2101 agcgataggg atgctgctct ggcgaaggtc ggccggctgt ctgaaaacta tgagcgactt
     2161 gtagacgagc acgaacgcac caaacgcagc gtgaagcggc tccatgataa agcgctcaga
     2221 ttcgcttctc aggcgatcga ggcgcgtcgt tcgttgattg cgaggctgcg cttgaggaat
     2281 gcgcgtcgaa aagggagcta gggaatgtat cattacaact tggatagtct aatttttcgc
     2341 gatcgcttgg cgtttggttg gggatggttt gtggacctgg aagcccgtgt cttcccggtg
     2401 cgtgtcgtcc tgcaacatgc agatgacagc gtctccacaa tattatgtct gcgttccggg
     2461 acgcgtacag atgtcgctga tgcattcccc gatgtgaacc atgcggcagc gtctgggttc
     2521 atgatacagg gccgcttgga tagtgacagc cccgtgtcca cggccagact ggtgttggcc
     2581 ggcgtcgacg gtaatgtcgg cggcactgaa atagaattgg gtgcgttggc gtcaatgctg
     2641 cagcaggatg tcagggccac cgtgtcgcga gggatcagaa tcctgcgccg gcgtagcttc
     2701 agcgccaacc tgggcatcgt cgctaaaaga ctgcgaatcc atgttcgcga agcactcgcc
     2761 gctcgccgaa gcatctcggg gcgccggaac gctatattga ttctcgacca tgatttgggc
     2821 ggtggctcaa acgcctttcg ccgtcagatg atcggtcgtc tgcgccaaac ctcgcctgtt
     2881 tatctaatta ctcctcgagt acagacactc gactactatc taactctgta cgatggggtt
     2941 gtcgaatctg gtcaggttta cgaaaggcta gccgacctcc ttcagttttt ggatggagag
     3001 tcgtgggcat caatcgtcat caatgagttg gtctcttatc cagaccccat ggctatggtg
     3061 aaatggttga gcgctcgggc cagcgtggtg ccagcttcta cagattttgt tctctacctt
     3121 cacgatttct actcagtttg tccacaatgg actcttgtcg gacggcgcgg tgtctactgt
     3181 ggcgttcctg attttgaaga atgtacgcgt tgtatcaagt
//
LOCUS       DATMTI010000962         1820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_206671, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000962 DATMTI010000000
VERSION     DATMTI010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206671"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1554)
                     /gene="murD"
                     /locus_tag="VN039_15190"
     CDS             complement(139..1554)
                     /gene="murD"
                     /locus_tag="VN039_15190"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWV47344.1"
                     /translation="MGMVGVDLTQLAGARVTVVGLARSGVAAVRLLQEAGALVTVADR
                     KDRRELFEALGSLDQATTRLVVGSDYESALDQAELVVISPGVPYRSEALERVRRRGAK
                     VIGELDLASRFLSTPILALTGTNGKSTTVTLIGKMLQESGKRVFVGGNLGTAISEAAV
                     QSLRAMKMSRPCPYDALVVEVSSFQLETVEQFHPWIAAILNVTVDHQDRYESIDEYIA
                     TKNRIFENQTPSDYALFNLDDCRVAPLRRSVRASVLGFTRTQTLPSDLAGGTYLDRNR
                     IMTTIGGQTREICARSEVKIIGNHNIENAMVAATYALLSGCSLDVIRQVLSEFPGLEH
                     ALEVVRERRGIRFINDSKGTNVDATLKALESIDQPIWLIAGGRDKGGDFSRLASAIRQ
                     RVKRLVLIGEAAPLIANAMKGYQGVERVSTLREAVELAASEADAGDVVLLSPACASFD
                     MFADYQDRGRQFKALVQSLPE"
     gene            complement(1554..>1820)
                     /gene="mraY"
                     /locus_tag="VN039_15195"
     CDS             complement(1554..>1820)
                     /gene="mraY"
                     /locus_tag="VN039_15195"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWV47345.1"
                     /translation="LGAALGTVAVISKHELLLLMVGGVFVIEAVSVIFQVASYKSRGK
                     RIFLMAPIHHHFEMKGWEEPKVVVRLWIIAILLALLSLSTLKLR"
BASE COUNT          396 a          570 c          460 g          394 t
ORIGIN      
        1 gcctaacggc tgttgaaaaa gtccgccagc ggcgttctcg catcgcttag aggctcaccg
       61 tacagcacaa agtacgcttc gtctcttcgt ttgctgcggc cttgccgacg gcctttttga
      121 acagcctgta ggctctcgtt actccggcaa agattgcacg agagctttaa attgccgccc
      181 tcggtcttga taatcggcaa acatgtcgaa actcgcgcag gccggcgaca gaagcaccac
      241 gtcgccggcg tccgcttcgg atgccgccag ctcaacggcc tcacgcaatg tgcttacccg
      301 ttcgaccccc tgataccctt tcatcgcgtt cgcgatcagc ggtgctgcct cgccgatcag
      361 aacaagccgc ttcactcgct gacgaattgc ggaggcgagc cgagaaaaat ctcccccttt
      421 gtcgcgtcca ccggcgataa gccaaatcgg ttgatcgata ctctccaatg ctttgagtgt
      481 cgcatccacg ttggtgccct tggagtcgtt gataaaccgt atgccccgtc gctcacgaac
      541 gacctccaac gcatgttcca atccaggaaa ctcgctgaga acctggcgaa tgacgtcgag
      601 tgaacacccg ctcaaaagcg cataggttgc agccaccatg gcgttttcga tgttgtgatt
      661 gccgatgatc ttgacctcgc tccgcgcaca gatctcccga gtttggcctc caatcgtcgt
      721 catgatgcga ttccgatcga gataggttcc ccctgccaga tcggaaggca atgtctgggt
      781 ccgcgtgaaa ccgagtacgc tggccctcac gctccgccgc agtggagcta ccctgcaatc
      841 atccaagttg aagagggcgt agtcggatgg agtttgattc tcgaatattc tgtttttggt
      901 cgcgatgtat tcatcgatcg actcatagcg atcctgatgg tccaccgtca cgttgagaat
      961 cgcggcaatc catgggtgaa actgttcgac ggtttccagt tggaagctgg acacttccac
     1021 caccaaagcg tcgtacgggc atggacggct catcttcatg gctcgcagcg attgtacggc
     1081 ggcctcactg atcgccgtgc ccagattccc gccgacaaat acccgctttc cgctctcttg
     1141 cagcatcttt ccgatcagtg tcacggtcgt gctcttccca ttggtgccgg tcaacgccag
     1201 gatcggagtg gaaagaaacc gcgatgcgag atcgagttcg ccgatgacct tcgctcctcg
     1261 acgacgaaca cgttcaaggg cctccgatcg atagggaacc cccggactga taacgacaag
     1321 ttcagcctgg tcgagagccg attcataatc gctgcccacg acgagacggg ttgtcgcctg
     1381 atccagagat ccaagcgctt cgaacaactc tcttcgatct ttccggtcgg ccaccgttac
     1441 caacgcaccg gcttcctgca ataagcgaac agctgctacg ccgctccgtg ccagcccaac
     1501 gactgtgact cgagctccgg ccaactgcgt gaggtccact cccaccatcc ccattacctc
     1561 aacttgagcg tgctcaagct caatagtgcc agcaaaatgg cgatgatcca caggcgtacg
     1621 acgactttgg gctcttccca gcctttcatc tcaaaatgat ggtgaatcgg agccatgaga
     1681 aagatccgct ttcctcggga tttgtatgag gcgacctgaa aaatgaccga gacggcctcg
     1741 atcacgaaga caccgccgac catcaacaac aacaactcgt gcttgctgat gaccgcaacc
     1801 gtcccaaggg ccgccccaag
//
LOCUS       DATMTI010000963         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_843298, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000963 DATMTI010000000
VERSION     DATMTI010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843298"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VN039_15200"
     CDS             <1..718
                     /locus_tag="VN039_15200"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HWV47346.1"
                     /translation="KQLARRIRASDSSLVVAVGLKAALAAKLEIVDIPILYMMILDPM
                     KHHLATANMTGVLLDIPTDRQLKVMRAFLPSVRRIGTLYDPNKTSTKLKEAESRASSH
                     EFQLRGFPVENEKEVPQQLRSLLSESEALWLIPDSTVLTDESIRFILQSAVAGQVPVV
                     GFSSEFTRLGALLSMSIAYGEVGHEAGLLAKRILNGEQRLPLNPVSVQRIRITVNQKT
                     ARYLGIAIPKEFDGLIDETY"
     gene            705..>2611
                     /locus_tag="VN039_15205"
     CDS             705..>2611
                     /locus_tag="VN039_15205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWV47347.1"
                     /translation="MKRIDGPTVGTTLQSSQSRPTVRLFGLRMKFVVFFGLILIMTCS
                     SLSWYFIETRRMVMTEHLEELGSILLTNTVHNEHFRIAGIVLEDRVTLGQFMQSLMAI
                     DHVVYVIITASDGRILDRQSKRTRRLSNGPRPTSEQPLYPDDRISQSLLRAPLTAPLM
                     TRLIQSAEQTLVPQNESSDWLLPFLVRNETVFDFAMPVLRESSSAIPLPHLSIELEDK
                     IGPPLLSTTSPVVGLVRIGITDAQAKQALLVIVRTVSILTLLIIVAGILGAHFLTSRI
                     TTPLRSLANSARQLARGNDAPVRLTVSTNDEVGELTQVFNGMTESLHDRNEAITMNLE
                     TIRRQVRQLTTVHQASTAIASASMFDMNRLLDTVLQLLVENLGFSKMTVFLYYPERNC
                     VSLARIIGVSPEIAEAIRRIDIPVTDNGGITSDLILHGKPLLIHDIETVTQRVHPPLL
                     EMARRSGVRSFVAVPLQSHTKILGFLAGDRSTRQCTDEDLHILLTIAGHVAAAIDNAK
                     AYSDLAELTQHLEERIEQRTEELSRANAQLQDHDRRRSTFLSVVSHELRTPMTAIRSF
                     AENMLDGVTGPLTDLQHTYLTRIQHNVARLGRIIVQLLDWSRLDTKRIELRMEEVSIY
                     QTATIVAESLQ"
BASE COUNT          584 a          783 c          658 g          586 t
ORIGIN      
        1 aaaacagctt gccagaagga ttcgtgcttc tgattcttcc ctcgtggtcg ccgtcggctt
       61 gaaggcggca ctggcggcta agttggagat cgtggacatc ccgattctct acatgatgat
      121 tctcgacccc atgaagcatc acctcgccac ggccaatatg accggcgttc tgcttgacat
      181 tccgacggac cgccaactca aggtcatgcg cgcatttcta cccagtgtgc ggaggatcgg
      241 cacgctgtat gatcccaaca aaacttcgac caagctgaaa gaagcagaat cgcgggcttc
      301 ttcacatgag tttcaattgc gggggttccc ggttgaaaac gagaaagaag ttccgcaaca
      361 actacggtcg ctcttgtccg aatcggaagc gctgtggctc attccggact caaccgtcct
      421 gacggacgaa tcgatccgct ttatcctcca gtcggcagta gccggacagg tgcccgtcgt
      481 cggcttttca tccgagttta cgcgcctggg ggcgttgctc agcatgtcga tcgcctacgg
      541 ggaagtcggc catgaagccg gtctcctcgc caaacgcatt ttgaacggcg aacaacggct
      601 gccgctcaat cctgtctcag ttcagcggat caggatcacg gtgaatcaga agactgcacg
      661 gtatttgggc attgcaattc cgaaagagtt cgacgggctg atcgatgaaa cgtattgacg
      721 gacccaccgt gggaaccact ctccagagtt ctcaatcaag accgacggtt cggctcttcg
      781 gtctccgcat gaagtttgtg gtgttttttg gactcatcct catcatgacc tgttcgtcat
      841 taagctggta tttcattgaa accaggcgaa tggtcatgac cgaacacctg gaggaactcg
      901 gaagcatctt attgaccaac accgtacaca acgaacattt tcgcattgct ggcatcgttc
      961 ttgaggatcg cgtcacactc ggtcaattca tgcagagtct gatggcaatc gaccacgtcg
     1021 tctacgtgat catcaccgcc tccgacggtc gtatcttgga ccgacaaagc aaacgcacca
     1081 ggagactatc gaacgggcca cggccgacat cagaacagcc gctctacccg gacgaccgga
     1141 tatcacaatc gctgctccga gctccgctga ccgctccgct catgaccaga cttattcaat
     1201 ccgcggaaca gacattggtt cctcagaatg agtcatcgga ctggcttctg ccgtttcttg
     1261 tgaggaacga aaccgtgttc gacttcgcca tgccggtcct tcgggaatcc tcatctgcga
     1321 ttccgctacc ccatctttcc atcgaattgg aagataaaat cggccctcct cttctatcca
     1381 caacttcgcc ggtggtcggc ctcgtgcgta tcgggatcac cgatgcgcaa gccaagcaag
     1441 ccttgctggt catcgtccgg actgtttcaa tcctcacgct gctcatcatt gttgccggca
     1501 tcctcggcgc acactttctg acatctcgta ttacgacgcc gctacgaagt cttgccaact
     1561 ccgctcgcca gcttgcacga gggaacgatg caccggttcg gctcactgta tccaccaacg
     1621 atgaggtcgg tgagttgaca caggtgttca atggtatgac ggaatccttg catgatcgca
     1681 atgaggccat caccatgaat cttgagacga tccggcggca ggtcaggcaa ttgaccactg
     1741 tgcatcaggc cagcaccgcg atcgctagcg ccagcatgtt cgacatgaac cggctgctcg
     1801 acacggtgct tcagttgctt gtcgagaatc ttggtttttc aaaaatgacc gtctttcttt
     1861 actatccgga gaggaactgc gtctcgctcg cccgaatcat cggcgtttcg ccggaaatcg
     1921 cggaggctat tcgccggatc gacatcccgg tcaccgacaa cggcggcatc acgtccgacc
     1981 tcatccttca cggcaagccg ttattgatcc atgacattga aaccgtaact cagcgcgtcc
     2041 atccaccgct ccttgaaatg gcgcgccgtt ccggcgtgcg ctcgttcgtt gcagtaccgc
     2101 tacaaagtca caccaagatc ctgggatttc tcgccgggga ccgcagcaca cgccaatgta
     2161 cagacgaaga cctccatatt ctcctgacga tcgccggcca cgtggcggct gccatcgata
     2221 atgccaaggc ttactctgat ctggctgagc tgactcagca tctcgaggaa cgtattgaac
     2281 agcgtactga agagctgtcc cgcgccaacg cgcaactcca ggaccatgac cgacgccggt
     2341 cgacgtttct ctccgtcgtc tcacacgagc tgcggacgcc catgacggcg attcgaagct
     2401 ttgcggaaaa catgctcgac ggcgtcacgg gtccgctgac cgatctccaa cacacgtacc
     2461 ttacgcgcat ccaacacaac gtcgccagac ttgggagaat catcgtccaa ttgctcgatt
     2521 ggtcgcgcct cgataccaag agaattgagc ttcgcatgga agaagtttct atttatcaga
     2581 ccgccacaat agtggcagag agcttgcaga t
//
LOCUS       DATMTI010000964         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_672196, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000964 DATMTI010000000
VERSION     DATMTI010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672196"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..1182)
                     /locus_tag="VN039_15210"
     CDS             complement(382..1182)
                     /locus_tag="VN039_15210"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWV47348.1"
                     /translation="MSDSLASFVTLILFRKWKMMIDSLANAFALEGIAIYWGIGLLFF
                     AAEYWWSTRPVPYLQVFLPDVAALTTYQVFFVFAAEVTNRIPFPHYSYWRWQALPFGF
                     KLIVFLFVVDGIAYWMHRLWHTSWGWPIHRWHHAPTELYWLAGVRASFPQIVLANVPY
                     LLAFPLLKPVPAAFFPIYGYMLVLTNNWMHMNVTWQSRKLEWLFVTPRYHRVHHLKDF
                     GKAGANFGVLFTVWDRLFGTYVDPEQVGLTGPYGISEEVHPIRMAVGV"
     gene            complement(1423..2514)
                     /gene="ychF"
                     /locus_tag="VN039_15215"
     CDS             complement(1423..2514)
                     /gene="ychF"
                     /locus_tag="VN039_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWV47349.1"
                     /translation="MGLCCGMIGLPNVGKTTVFNALTGGGALAANYPFATVDPNTGIA
                     LVPDPRLIKLTEIFTSKKTTYSTLEVRDIAGLVEGASKGEGLGNQFLGHIREVDALLH
                     VVRCFQGTDVVHVSGGIDPLRDIGVIETELMLSDLETLDRRKQKTEKKVRAGDKKAAF
                     EVEFLAKLIGLLDKGEWLGNREYTVEERVILNECQLLSAKPVLFVANVSEGKNADEAM
                     VQTVREFAAKRGARVVTICGQLEAELSSLPESERADFLNELGLTESGLVRLTREAYTL
                     LDLITFFTAGEIESRAWPIPKGTKAPQAAGKIHSDMERGFIRAEVYHYDDLLTCGSEA
                     KVKEKGLFRLEGKDYVIKEADIVYFRFNV"
BASE COUNT          649 a          773 c          729 g          634 t
ORIGIN      
        1 ggccgtggcg cgtgccatgt tgaagacgat ctatgcgatg ctgacgaagc aggaggcatt
       61 tcacccgatg gcgaagggaa ccaccagtca gcgccacggg gtcatggccc gctagcctct
      121 aggctgagcg aggtcccgag acttgatggc gcgaccggtg tcccatttca tcctgggccg
      181 tgagcccgca gaggtgactg actcgtcgtc cactgagacg cgcggagaac cggatcgcgg
      241 acggtcgtgc ttgctcttga ccgtcgtttt cataggtgac cctatttcac gacctttgta
      301 aaaatggggg tctgaccgtt ttctattgac cgtttactga acacggcggc gagtcatggt
      361 tgattaagat cgcttctccc cttagacccc gactgccatg cggatgggat ggacttcttc
      421 tgatattcca tagggccctg tcagacccac ttgctcagga tcgacatacg tcccgaacaa
      481 ccgatcccat acggtaaaca ggaccccgaa atttgctccc gccttcccaa agtcttttaa
      541 gtggtgcaca cgatgatagc gaggcgtgac gaagagccac tccagttttc ttgattgcca
      601 ggtcacgttc atgtgcatcc agttattggt taggaccaac atatagccat agatcgggaa
      661 aaacgctgca ggcaccggtt tgagtagtgg aaacgccaaa agatatggaa cattcgcgag
      721 gacgatttgc ggaaagctcg cccggacacc ggccagccag tacaattccg ttggcgcatg
      781 gtgccatcga tggatcggcc agccccacga cgtatgccaa agccgatgca tccaataggc
      841 gattccgtcg acgacgaaca agaacactat tagcttgaaa ccgaatggaa gcgcctgcca
      901 acgccaatag gagtagtgag gaaacgggat tcggttcgtc acctctgcgg caaagacgaa
      961 gaagacttgg tacgtggtga gggctgccac atccggtagg aacacctgga gatatgggac
     1021 cggacgtgtt gaccaccaat actctgccgc gaaaaagagc aggccaattc cccaatagat
     1081 ggcaatccct tccagcgcaa acgcattagc aagcgagtcg atcatcattt tccacttacg
     1141 gaataggatt aaagtaacaa atgaggcaag agaatcgctc atcttgctgg tgccggtcaa
     1201 gtcagcatgg aaccccatta gtaggggtga agcgtcatca actaatccga tacggtcaag
     1261 aaccccctaa gagaaacgca aaacggctcg gcacagataa aggttgaggg tttgttctca
     1321 gatcactggc agtttgtcgg tctatactat gcagtggagc gttgggctcg gtgatcaacg
     1381 tcgaaagtta gccgaagaga attgtgcggt agttgaggaa gactaaacat taaacctaaa
     1441 atacacgata tccgcttctt tgatgacgta gtctttccct tcgaggcgga acagtccctt
     1501 ctccttgacc ttcgcttccg acccacaggt gagcaaatcg tcatagtggt agacttctgc
     1561 acgaatgaag cctcgttcca tgtcggagtg gatcttgcct gcggcttgag gcgccttggt
     1621 gccctttgga atcggccagg ctcgagattc gatctcgccg gcggtaaaga aggtaatcag
     1681 atccagtaga gtataggctt ctctagtcaa tcgaacgagc cctgattcgg tcagccccag
     1741 ttcgttcagg aagtccgccc gttcgctctc aggcaatgac gacagctcgg cttccagctg
     1801 tccgcagatg gtcacgactc tagccccgcg cttggccgca aactcgcgca cggtctgcac
     1861 catggcctcg tccgcattct tcccttcgga cacgtttgcg acaaacagaa ccggcttagc
     1921 ggagagcaat tgacattcgt tgaggatcac acgttcttcc accgtgtatt cccgattgcc
     1981 caaccattca cctttgtcga gcaatccgat gagcttggca aggaactcca cttcaaacgc
     2041 agctttttta tcaccggccc ggaccttctt ttcagtcttc tgttttcggc gatcgagtgt
     2101 ttccagatcg gacaacatga gttcggtttc gatcacgccg atgtcacgaa gcggatcgat
     2161 cccgccgctg acatgaacca catcggtgcc ctgaaaacag cggaccacgt gaagaagggc
     2221 atccacttct ctgatgtggc caagaaattg attgccgagg ccttctcctt tgctggcccc
     2281 ttcgacaagc ccggcaatgt ctcgtacttc caacgtgctg taggtggttt tcttcgaggt
     2341 gaatatttcg gtcagtttga tgaggcgggg gtccggcacc agggcaatac cggtgtttgg
     2401 atcgacggtt gcaaacggat aattagccgc cagggcaccc ccaccggtca gtgcattgaa
     2461 gaccgtcgtc ttgccgacgt tcggcagacc gatcatgccg cagcaaagtc ccatgttatt
     2521 tccttgtctt tctacacact ttttacaaag gggagctgcg gggctgacct aactgcgcgc
     2581 atccaccgag caccgccctt agtttcgtca ttcgatccat tctcgcgtgc gcggtgggcg
     2641 agcacaggtc agtccggctg ctccccttca tctactcgct ctctgacatt aaactgattc
     2701 atcgccactt cgggtccgcg atgaatcaaa cattccaata catccactgc ccgttccagg
     2761 cagggatcaa agacagccat ctcat
//
LOCUS       DATMTI010000965         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_587094, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000965 DATMTI010000000
VERSION     DATMTI010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587094"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VN039_15220"
     CDS             <1..299
                     /locus_tag="VN039_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47350.1"
                     /translation="RKEFEPQMFPPITLRVIGVKIPGNFYVVLQSIECLIQQRTEHTG
                     GQRLIEGFIGVERFRLSRPNRSGQTHRHNRGDQYFHQGMTVIFEQKPVKIQKRL"
     gene            complement(306..914)
                     /locus_tag="VN039_15225"
     CDS             complement(306..914)
                     /locus_tag="VN039_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47351.1"
                     /translation="MREDSVCSNRSAHASQRVVSVLICVVITALGLLSAPAFAGPQVP
                     FSASGTILTIDTGDVRPAGQSGRFIVRDRHITGTLTGSIGGTTGVPFAITYDSNVPIA
                     TQSGRIHARMIAGVYEANMTLASSTGPTPVECETPNGLSCIATPGGNFVPGLLLNGRM
                     NFLSGAKGQGTVTGWLIPILDEQGHIVSIAASLLTLRGQWSQ"
     gene            complement(1130..1555)
                     /locus_tag="VN039_15230"
     CDS             complement(1130..1555)
                     /locus_tag="VN039_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWV47352.1"
                     /translation="MPSAKTTKFALLDTAIYIENFRSGRFTFRLLQSPYIIRCSSVVL
                     HELLRGARAATERKFVFELSRRCQVLTPTEQQWLRAAEILLRLRRQEHYEVAKLRDLA
                     FDVLIALSARSIGASVITTNHEDFRAIQRELPFHLLCWE"
     gene            complement(1533..1727)
                     /locus_tag="VN039_15235"
     CDS             complement(1533..1727)
                     /locus_tag="VN039_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47353.1"
                     /translation="MALIQVSARLNPIQLRRAQKALGAKTTSETLQRALDLVTEKAAH
                     DRIIQRYSGVGKPDAFGEDH"
     gene            complement(1807..2565)
                     /locus_tag="VN039_15240"
                     /pseudo
     CDS             complement(1807..2565)
                     /locus_tag="VN039_15240"
                     /inference="COORDINATES: protein motif:HMM:NF042585.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6733 family protein"
BASE COUNT          602 a          732 c          709 g          548 t
ORIGIN      
        1 tccggaaaga atttgaaccg cagatgtttc ccccgatcac ccttcgtgtc atcggagtga
       61 agattcccgg aaatttctac gtggtcctcc aatctatcga gtgcttgatt cagcagcgaa
      121 cggagcatac cggtggtcag cgcctgattg aagggttcat cggggtcgaa cgattccgcc
      181 tcagtcgccc aaaccggagc ggccagacac atcgacacaa tcgcggtgat caatattttc
      241 atcagggcat gactgtaatc ttcgaacaga aaccggtcaa gattcagaaa agattgtagg
      301 gagcgtcatt gactccactg acccctaagg gtcaagaggc ttgccgcgat actcacgata
      361 tgcccttgtt catcaagaat cgggatcaac cagccggtca cggtgccctg gcccttggcg
      421 ccgctgagaa agttcatcct gccgtttagc agcaggccag ggacgaagtt tccacccggt
      481 gtcgcaatgc acgaaagccc gttgggcgtc tcgcactcca ctggggtggg acctgtgctt
      541 gatgccagcg tcatattcgc ttcatacacg ccggcgatca tgcgcgcatg aatgcgaccc
      601 gactgcgtag cgataggcac attggaatcg taggtgatgg cgaaaggaac gccggtcgtg
      661 ccaccgatgg agcccgtcaa ggttcccgta atgtggcgat ctcttacgat gaaacgtccg
      721 ctttggcccg ccggcctgac atcaccggta tcgatcgtca ggatagtacc actggcgcta
      781 aacggcactt gcgggccggc aaatgccggt gcggaaagga gcccgagcgc ggtaatgacc
      841 acacaaatca aaaccgacac tacacgctga gatgcgtgcg ctgaccgatt gctacaaacg
      901 ctgtcttcac gcatatcagg gctccttgga gtgaagaaag actccgggcc tagatgaatg
      961 actatctctt gtaacgtatg ggccgaaaga acacaacctg gtccctattt tcttttcgaa
     1021 tttataggga agccctctgc ggaacagggc tttgggcatg acgcacggaa gcgggttacg
     1081 gcttcttgct taaccttctc ctgctttggc accccatcaa aactttgctc tattcccaac
     1141 acagaagatg aaagggcagc tcgcgctgaa tcgcgcgaaa gtcttcgtga ttggtcgtga
     1201 tcacactggc gcctatactc cttgccgaca gggcgatcaa cacatcaaag gccaggtcgc
     1261 gcagcttggc gacctcatag tgttcttgtc gtctgagccg aaggaggatc tcggcggccc
     1321 tcagccactg ctgctccgtt ggcgtcaaca cttggcaccg ccgactcaat tcaaaaacaa
     1381 acttccgttc agtggccgct cgagccccgc ggagcaattc gtggagcacg acggaagaac
     1441 aacggataat ataaggagat tgcagcaggc gaaaggtaaa gcgtccggat cggaaatttt
     1501 caatgtagat cgcggtatcg agcagcgcga acttagtggt cttcgccgaa ggcatcgggc
     1561 ttgcccacac cgctatagcg ctggatgatg cgatcgtgcg cggctttttc cgtaaccaaa
     1621 tccaaggcgc gctgcagtgt ttcgctcgtt gttttcgcac cgagcgcttt ttgggctcgc
     1681 cgaagctgta tgggatttaa cctggcggac acttgaatga gcgccatgca ccacctcctg
     1741 tcggacagag taccaaattc tgtccgacag cgtcaagaca tccacgccgc tcacaatgaa
     1801 gacggactag aagaccttgg tgaagcgaac cttgtaaaag tctccggacg gattagcatt
     1861 cagggcgcct gtcgttccat tcgggtttat cgtgtgcaca ctctctgcga agtctttgcc
     1921 cgctgcaaac atgaccgaca tccccatcgg catccggagt tcaacatagc cgccaagcca
     1981 ggcgaccatc ccctgcggcg gcagaacgga tgcgttctga ccatttgcga gacagccagt
     2041 gcaggggtgt cctccgctgc tgaactgatg gatgtcctca tagtagccac ccacgatgag
     2101 cttcgtgccg ctctcggtct tgatgaccgg gactccggca tagagccaat ggtgggtgaa
     2161 cgaactctgg gtcgggcctt gtttcctgac atcctcgatc agcacgaagt acccatccac
     2221 atccagcagt tccccggcat agtgggcctg gaacaggaac aggaacgcat caaagacatt
     2281 cgcgtggcat ccaccgttac tccccaactg tgcggcaccg caggagttga cctggccgtt
     2341 cgtgaatccg acctggggac ggagcaccag tttgccgtcc atgaaggact tgtggattcc
     2401 accataaacc caggtccaag gattgctcca gtccggcctc gggttggaca agtgcccata
     2461 gagcgtagag ccgaaggcga tgccgaaatc ctgtcgcagg tagtaggtgc cggcaatgat
     2521 cggatagaac ccgaagaact tatcctggtt cagggacacc gagacattga agcgatcgaa
     2581 ttccttttaa g
//
LOCUS       DATMTI010000966         5598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_757288, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000966 DATMTI010000000
VERSION     DATMTI010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757288"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VN039_15245"
     CDS             <1..1094
                     /locus_tag="VN039_15245"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWV47354.1"
                     /translation="RIEAIPGSEQVSKKRAGRFDLPRDPKNNAIIGDSRNDENVVISQ
                     FHLAMLRFHNAVVDQLRSDPAQANRSARDIFEEARRQVQWHYQWIILNEYLPLTIGQD
                     RVDEILQNGPRFYRIQDPLFRNSRGDLLIPVEFAVAAYRFGHSQVRPTYQLNFGPDAG
                     PPVVLSIFDDSADPNDPDPNDMRGGKRAPRRFIDWQTFFKFNPDISALNKKIDTKLSS
                     PLMLLPGSRGPAPGLPADGIQSLASRNLMRHVNFGVPSGQAIAKVMGIPVLTPGQLAE
                     LAPFGMDRSTPLWYYILKEAEVLENGLHLGPVGSRIVGEVFIGVLKADKDSYLVVDKN
                     WKPTLPSAKVGDFEITDLLTFAGVVHPLN"
     gene            1330..2118
                     /locus_tag="VN039_15250"
     CDS             1330..2118
                     /locus_tag="VN039_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HWV47355.1"
                     /translation="MLITETSEEGSRIAGSLVAQTITQTPALRLGLAAGNTPIGLYRN
                     LVDLHRTTHLDFSRVRLFSLDEFIGLRSDSPNHYRVFYHQHLIDHLNIDPAHLHLLSG
                     PAEQDIASSCVSYERLIQNQGGIDLQILGIGRNGHLGFNEPGSSLNSRTRRILLSAST
                     KQNLGRVFDGEPVPEWAVTMGLGTIREARTLLLLAFGAHKAEVVAKAVEGPLSAFTPA
                     SSIQLHPSVVLVLDREAAGSLTNQEYYRQQSSQVMKGDQWFLEL"
     gene            complement(2299..3732)
                     /locus_tag="VN039_15255"
     CDS             complement(2299..3732)
                     /locus_tag="VN039_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWV47356.1"
                     /translation="MGDPKGFMKYAREGPKRKPIELRVLDWKEMYEPISEDKLKTQGA
                     RCMDCGVPFCQGHTGCPVVNLIPEWNDLVYRGRWKDALKALHTTNNFPEFTGRLCPAP
                     CEGACVLGINEDPVSIRIIEWNIVDRGFNDGLVTPILPAVRTGKSVAIVGSGPAGLAA
                     AQQLARAGHDVTVFEKADRIGGLLRYGIPDFKMEKWVIDRRLEQMKAEGVKFQTGVVV
                     GEDITGEQLRQRFDAVGLTMGAEQARELPIPGCELKGVHLAMEYLTQQNKRTAGIPFS
                     DEPITAKGKRVVIIGGGDTGSDCLGTAHRQGCLEAHQFELLPEPPPERAESTPWPLWP
                     MQLRTSHAHEEGCDRQWSVSTTKFTGHDGHVTKLHAHRVRFSNGKFVSVSDTDFEMNV
                     DLVLLAMGFTGPVKNGLLDNLGVKYDARGAVAVDENLMTEREGVFAGGDTKRGASLIV
                     WAIAEGRKMAAGIDKYLQAGKSAKAVA"
     gene            complement(3825..>5598)
                     /locus_tag="VN039_15260"
     CDS             complement(3825..>5598)
                     /locus_tag="VN039_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HWV47357.1"
                     /translation="DPERFIPLPNGDSKNSYIKQVASARFGVTAHYLANARELQIKMA
                     QGAKPGEGGQLPGHKVDENIAKFRYATPGVQLISPPPHHDIYSIEDLAQLIFDLKNSN
                     PDAGVSVKLVAEVGVGTVAAGVAKAHADKVLISGDSGGTGASPLSSIKYAGIPWELGL
                     AETHQTLVLNDLRGRIRVETDGQMKTGRDVVIATLLGAEEYGFATAPLIVEGCIMMRK
                     CHLNTCPVGIATQDPELRKKFNGKPEHIVNYLFFVAEEARQLMARLGFRTINEMVGRV
                     DKLKITRAVGHWKAKGLDLTPLLTIPDVSADVPRYCVQKQDHGLTDILDNKLVELCKA
                     AIERGEKVKLELPIRNINRTTGTVLSSKIAKKYGPEGLPEDTISIKFTGSAGQSFGAF
                     LAKGITLTLEGESNDYIGKGLSGGKIIVFPPKNILYNPEETILIGNTSLYGATQGEAY
                     FYGMAGERFAVRNSGAQAVVEGTGDHGCEYMTGGVVVVLGKTGRNFAAGMSGGTAFVL
                     DDAGTFQSRCNTGMVELEPVTTKDDKQLLHGLITKHFMYTGSRKAKQVLDAFEAILPK
                     FVKVMPVDYKRVLEERKRKASAVH"
BASE COUNT         1151 a         1675 c         1517 g         1255 t
ORIGIN      
        1 tccgcatcga agccattcct ggatccgaac aagtctccaa gaaacgcgcg ggacgattcg
       61 acctcccgcg cgatccaaaa aacaatgcga tcatcggaga cagccggaac gatgaaaatg
      121 tcgtcatcag tcaattccac ctggcgatgc tgagatttca caacgccgtg gtcgatcagt
      181 tgcgctcgga tccggcgcag gcaaatcgtt cggcccgaga tattttcgag gaagcacggc
      241 gccaggttca gtggcactac caatggatca ttctcaacga atacttgccg ttgacgatcg
      301 ggcaggatcg ggtggatgag atcttgcaga acgggccgcg tttctatagg attcaagatc
      361 ccctgttccg caattcgaga ggcgatctgc tgatcccggt ggaatttgcg gtggccgcat
      421 accggttcgg ccattcgcaa gttcgtccca cttatcagtt gaattttgga ccggatgcag
      481 ggccgccggt cgttttgtcc atcttcgacg attcggccga tccgaacgat cccgatccta
      541 atgacatgcg cggcggcaaa cgggctccac gtcgattcat cgactggcag accttcttca
      601 agttcaatcc cgacatctcc gcgctcaaca agaagatcga caccaaactg tcgagtccgc
      661 tgatgctcct gccggggtca cgcggaccgg cgcccggttt gccggcggac ggaatccagt
      721 cgctggcctc tcgcaatctg atgaggcacg tcaattttgg tgtcccgtca gggcaagcga
      781 tcgccaaagt gatggggatt ccggtgctga ctcccggaca acttgccgaa cttgcccctt
      841 tcggcatgga ccgaagtact ccactctggt attacatcct gaaggaagcg gaagtactcg
      901 agaacggctt gcacctgggg ccggtcggaa gccggattgt cggggaggtg ttcatcggcg
      961 tactcaaagc cgacaaggat tcgtatctcg ttgtcgataa gaactggaaa ccgaccttgc
     1021 cgtcggcaaa agttggagat ttcgagatca ccgatctgct gaccttcgcc ggagtcgttc
     1081 atccgctgaa ctagatcctg ctgtaacgcc tcgatgggta ccctctggag aggtacccaa
     1141 gtttaaaagc gtaaaagctg tggccggcga ctttctgacc aacaatgctg tgcctccaat
     1201 ggatgcttcc tcgcctcggg ctgggaatat gctgcgatgg ttggaacgga aagaatacgt
     1261 ccaactgtat gaacaaatga tccgcgaatc gtcggtgaac gaatcattct cttcgaatga
     1321 atgatgctgg tgctcatcac agaaacatca gaggaaggca gcaggatagc gggaagcctc
     1381 gtcgcccaga cgatcacgca gaccccggca ttacggctcg gactcgctgc cggcaacacg
     1441 ccgatcggat tgtaccggaa cttggtggac ctacaccgca cgacgcatct cgatttttct
     1501 cgcgtccggc tttttagtct cgacgagttc atcgggttgc ggagcgacag cccgaatcac
     1561 tatcgtgtgt tctatcatca gcatttgatc gaccacctca atatcgatcc ggctcatctc
     1621 catctgttga gcgggccggc cgagcaggac atcgcctcct cctgtgtttc ctatgagcgg
     1681 ctcatccaaa accaaggcgg cattgatctg cagatcttgg ggattggacg gaacggccac
     1741 cttggattca atgaaccagg ttccagcctc aattcccgaa cgaggcgcat tctcctgagc
     1801 gcgtcgacca aacagaatct tggtcgggtc tttgatggtg agccggtccc ggaatgggct
     1861 gtaaccatgg gtctcggtac tattcgtgag gcgcgaactc tcttgctgtt ggcctttgga
     1921 gcacataagg ccgaggtcgt cgccaaagcc gtcgaaggac cgttgagcgc tttcacacct
     1981 gcctcctcca ttcaattgca tccgagtgtc gtcctcgtgc ttgatcggga agcagcgggt
     2041 tccctcacca atcaggaata ctatcggcag caatcgtctc aggtgatgaa aggcgatcaa
     2101 tggttcctgg aactttgatt tacgcaggct actcacagcg ggtaagacct ttgatctccc
     2161 accgaatccg ccgttatggg tttgcgcgag gcaagaaccg gtccggtgaa agtcgcgtct
     2221 ttagcgcgac ggggtgggcg ggtgagaagg aagcagtaaa tatggagaag aaacctgggc
     2281 tttatgttgc gaggcctttt aggcgactgc tttcgcggat tttccagcct gcagatactt
     2341 atcgatcccc gccgccatct tgcggccttc ggcgatggcc cagacgatga gtgaggcacc
     2401 gcgtttggta tcgccgccgg cgaagacgcc ttcacgctca gtcatgaggt tctcgtccac
     2461 cgccacggct ccgcgcgcgt catacttcac gcccagattg tcgagcaggc cattcttgac
     2521 gggaccggtg aatcccatgg cgagcagcac cagatcgaca ttcatctcga agtcggtgtc
     2581 ggagacagat acgaatttac cgttactgaa ccgtacgcga tgcgcatgca gtttggtgac
     2641 atgaccatcg tggccggtaa acttcgtggt ggagacgctc cactgccggt cacagccttc
     2701 ttcatgtgca tgcgacgtgc gcagctgcat cggccagagc ggccaggggg tcgactcggc
     2761 ccgctccggc ggcggctccg gcagcagttc aaattgatgc gcttcgagac agccttgacg
     2821 gtgcgcggtg ccgagacagt cggagcctgt atcgcctcct ccgatgataa ccacccgttt
     2881 acccttcgcg gtgatcggct catcgctgaa agggatgcct gccgtgcgct tattttgctg
     2941 ggtgagatat tccatcgcaa gatgcacgcc cttcaactcg catccgggaa tgggcaactc
     3001 acgcgcttgt tcggctccca tcgtaagacc tactgcatca aaccgttgac gcagctgttc
     3061 gccggtgatg tcttcgccga ccacgacacc ggtttgaaat ttcacccctt cggccttcat
     3121 ctgttccagc cgtctgtcga tgacccactt ctccatcttg aaatcaggaa taccgtagcg
     3181 aagtaggccg ccgatgcgat cggccttttc gaagaccgtg acatcatggc ccgcccgagc
     3241 cagctgttgt gcggcggcga ggccggccgg accggaaccg acgattgcca ccgatttccc
     3301 cgtcctgact gccggcagga ttggagtcac aagcccatcg ttgaagccac gatcgacgat
     3361 attccattcg atgatgcgga tggagaccgg atcttcattg atacccagca cacaggcccc
     3421 ctcacagggc gccggacaga gtctgccggt gaactcggga aagttgttcg tcgtatgcag
     3481 agctttaagc gcgtctttcc agcgaccgcg atagacgaga tcgttccatt cgggaatcag
     3541 attcacgacc gggcaaccgg tatggccttg gcaaaacggc accccgcaat ccatacagcg
     3601 cgcaccctga gtcttcaact tgtcctcgga gatcggctcg tacatctcct tccaatcgag
     3661 cacacgcagc tcgatcggct tgcgcttcgg gccctcacgg gcatatttca tgaacccctt
     3721 tggatcgccc atcttctctc caggactgag gattcaggac tgaggactga gaaattcgct
     3781 accccttcac ttggccctca gtcctcatag cgagcgaacg cgagctagtg cactgcactc
     3841 gccttgcgtt ttcgctcttc caagactcgc ttgtaatcca ccggcatcac cttcacaaac
     3901 ttcggcaaga tggcttcgaa cgcatcgagc acttgcttgg ccttacgact acccgtgtac
     3961 ataaaatgct tggtgatcaa tccgtggagc agctgcttgt cgtccttcgt cgtcaccggt
     4021 tccagttcga ccatcccggt gttgcagcgg ctctggaatg tcccggcatc gtccagcacg
     4081 aacgccgttc ctccggacat gccggccgca aagtttcgcc cggtcttgcc gagtaccacc
     4141 accacaccac cggtcatgta ttcacaaccg tgatcgcccg tcccttcgac gacggcctgc
     4201 gccccgctgt ttctgacggc aaaccgctca cccgccatcc catagaaata ggcctcccct
     4261 tgcgttgccc catagagcga cgtattgccg atcaaaatcg tctcttccgg attataaaga
     4321 atgttcttcg gcgggaagac gatgatcttg ccgccggaca atcctttacc gatatagtcg
     4381 ttggactcgc cttccagggt cagcgtgatg cctttcgcaa ggaacgcacc gaacgattga
     4441 ccggccgagc cggtaaactt aatcgagatc gtgtcttccg gcaacccctc cggcccgtac
     4501 ttttttgcaa tcttgctgga gagcaccgtg ccggtcgtcc gattgatgtt cctgatcgga
     4561 agctcgagct tgaccttctc acccctctcg attgccgcct tgcaaagctc gacgagcttg
     4621 ttatcgagaa tgtcggtgag tccatggtcc tgcttctgca cacaatagcg cggcacatcc
     4681 gccgaaacat cgggaattgt cagcagcggc gtgaggtcca gccccttggc tttccagtgg
     4741 ccgacggctc tggtgatctt gagcttgtcg acgcggccga ccatctcgtt gatcgtccga
     4801 aatccgagcc tcgccatcag ctgcctcgct tcttcggcca cgaagaacag gtaattgacg
     4861 atgtgttccg gcttgccgtt gaacttcttg cgcaattcag gatcttgtgt cgcgattccg
     4921 accggacagg tgttcagatg gcacttgcgc atcatgatac agccttcgac aatcagcggg
     4981 gccgtcgcaa acccatactc ttcagcaccg agcagcgtgg cgatgaccac gtcgcggccg
     5041 gtcttcatct gaccatcggt ttcaacacgg atacgcccgc gcagatcgtt gaggaccaat
     5101 gtctgatgag tttccgccag ccccagttcc caagggatcc cggcatattt gatcgacgac
     5161 aatggtgatg cgcctgtgcc gcccgaatca ccgctgatga gcaccttgtc cgcatgggcc
     5221 ttggcgacac cggctgcgac ggtcccgacg ccgacctccg ccaccagttt caccgataca
     5281 ccggcatccg gattggaatt cttcaagtcg aagatgagct gggccaaatc ttcaatcgaa
     5341 tagatatcgt gatgcggtgg cggcgagatc aactgtacgc caggagtcgc atagcgaaat
     5401 ttggcgatgt tttcgtccac cttgtgtccc ggcagctggc cgccttcgcc tggcttggct
     5461 ccctgcgcca ttttgatctg caattcccgc gcattcgcga ggtaatgcgc cgtgactccg
     5521 aatcgggccg acgccacttg tttgatgtaa ctgttcttgg aatccccgtt gggaagcgga
     5581 atgaaccgct cgggatct
//
LOCUS       DATMTI010000967         8814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_163482, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000967 DATMTI010000000
VERSION     DATMTI010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163482"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1241)
                     /locus_tag="VN039_15265"
     CDS             complement(105..1241)
                     /locus_tag="VN039_15265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWV47358.1"
                     /translation="MPDLSVIQTALNRAVTEGVFPGAVLAVRCGDRPVSRFHAGHLSD
                     VPPGPDVSTSTIYDLASLTKPLATVTSIALLIRQGDLRLEDTVAKYLPECAETSIGSA
                     TLRDLLTHSSGLPGWRPYYERINPEGEIPSSKEARDHAKQSMLSLIRSEAPVYERGTR
                     SLYSDLGFMVLGIIVERCSRQDLDHFFYDYITVPLDGLRIEYVLRERLNEFLESANRH
                     DGGVAPTEVDPWRGRLLCGEVHDQNAAALGGEAGHAGLFGTADAVLAITGEWLKAYHG
                     RKAILDQGIAREFTRRQKPEGSSSWALGWDTPSTPSSAGRYFGARSFGHLGYTGTSVW
                     IDPVQELEVVLLSNRVHPSSKNEAIREFRPAIHDLVYQEFIGSI"
     gene            complement(1319..1831)
                     /locus_tag="VN039_15270"
     CDS             complement(1319..1831)
                     /locus_tag="VN039_15270"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HWV47359.1"
                     /translation="MKITPLDIQQMVFQVKLRGYDREEVNRFLEEIAQTVEFLNRDNA
                     ILRDRIVSLEQQISELKRTEATLSTTLVSAQSLAEDVKRSAQRDAELIVKEAELKAGE
                     LFRQARVELGNTQRDLSVLQKQRLLMVERMRATLHTFERMLDIEASEVYQDTGVMQEE
                     KLEGESSPTR"
     gene            complement(1852..2148)
                     /locus_tag="VN039_15275"
     CDS             complement(1852..2148)
                     /locus_tag="VN039_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWV47360.1"
                     /translation="MFVVGNTLLGFATVLDYVLTFYTWIVIARALISWVNPDPWNPIV
                     QFLSRATEPVLAPIRRRLGWSMGVDLSPLVVIVAIWFLQIAVVQSIKDIAVRMN"
     gene            complement(2148..2966)
                     /gene="proC"
                     /locus_tag="VN039_15280"
     CDS             complement(2148..2966)
                     /gene="proC"
                     /locus_tag="VN039_15280"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWV47361.1"
                     /translation="MSSSTHSQSISFVGGGRMAEALISGVLSAREYKADQIHVADPDT
                     VRLDHLKRQYGIQIGLTNHEAVALGDIVVLAVKPQVTAEVLKEIGDVLTKRLVISVVA
                     GVRLGRIIEACGPQARVIRAMPNTPAMVGEGMTALAIGPGVGDNEIACARQIFESVGR
                     VVPIDERFMDAVTGLSGSGPAYVFLMIEAMADGGVKMGLPRETSSLLAAQTVLGAARM
                     VLETGQHPASLKDQVTSPGGTTIAGLHRLEQGGLRGLLIDAIESATKRSRELGG"
     gene            complement(2959..3699)
                     /locus_tag="VN039_15285"
     CDS             complement(2959..3699)
                     /locus_tag="VN039_15285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWV47362.1"
                     /translation="MSGRDSCEGTMEPLITEAIAKRVQSVLTKIRSAEEKAGRPAGTV
                     RLVAATKTVSVEHIAEGVRAGLSILGENRVQEALPKIAAFTQPPVHWHFIGQLQRRKV
                     RSVIGLFDLIHSVSSLDLAQEIDRRAGEAGRRQNVLLEVNIGKEPTKAGFHPDDVVRS
                     VPMMAQLSHVCVKGLMTIPPPTADPDSARPYFRTLRDLARQIAAFDLPTVRMGELSMG
                     MSNDYEVAIEEGATLVRVGTAIFGARDV"
     gene            complement(3754..4497)
                     /gene="pgeF"
                     /locus_tag="VN039_15290"
     CDS             complement(3754..4497)
                     /gene="pgeF"
                     /locus_tag="VN039_15290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWV47363.1"
                     /translation="MTRTSVITVPAFADTGSQVRHFFGTRRHAMGLGLEVGIPRKGIA
                     GTASSSWTLSVKQVHGTEALVVDRTVTSTDRFVGGWDALVTDQPGVMVAVRTADCVPV
                     LIHDPKRHVVAAVHAGWRGAVAGIVPKTLALLESRFGSRLEHVRISIGPSAGVCCYEV
                     DEPVLDHLCQEFPDWDKVVRTRGDGKANLDLKALVKEQAQALGAAPKFITTVNLCTIC
                     HEDLFFSYRRERKVNGTMVSAIGLPVRCG"
     gene            complement(4539..5735)
                     /gene="ftsZ"
                     /locus_tag="VN039_15295"
     CDS             complement(4539..5735)
                     /gene="ftsZ"
                     /locus_tag="VN039_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWV47364.1"
                     /translation="MFSFQEDLLSPVRIKVIGIGGGGCNAINTMITSGLARVDFIASN
                     TDLQALDRSLAPYKIQLGPERTRGLGAGAKPEIGRDAALESKEHIRECLEGADMVFVT
                     AGMGGGTGTGAAPIVASIAREMGILTVGVVTRPFQYEGKRRNKHAEEGIRDLRRHVDT
                     LLVIPNQRLLGIVDKSTPLLEAFKVADDVLRQAIQGIADVITTTGHVNVDFADVRTVM
                     SHTGRAVMGMGISYGPNRAIEAAQKAMCSPLLEEGSVEGARGVLLNITGGPSMSLHEI
                     EEAASIIQQTADPEANIIVGQVINPEMGEELIITVIATGFEREEDSAEATIGADRGMS
                     RPAKPVPSVLAGMGASLAVDRPMKDLDRPTFLRRMNDARESMDRAVLTAEDEWDVPTF
                     LRKQTD"
     gene            complement(5799..7031)
                     /gene="ftsA"
                     /locus_tag="VN039_15300"
     CDS             complement(5799..7031)
                     /gene="ftsA"
                     /locus_tag="VN039_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWV47365.1"
                     /translation="MPKRDQILVGLDIGTTKICAIVAEMTDAGGLSIIGVGSSPSRGL
                     RKGVVVDIESTVESIKKAVEEAELMAAVQINSVYTGIAGSHISAENCKGVVALKRAEV
                     TREDIQRAIESARTLAVIPHERRILHVLPREFMVDGQEGVREPLGLSGNRLEVNVHVI
                     TGAVTSAQNIVKSVNRAGLDVVDIILQPLASSEAVLSQEERDLGVAMVDLGGGTTDLA
                     IFLDGSIRHSAVLPIGGQNLTKDLAIGLLTSQTEAEKIKTQYGVARTELVTGHQIVEV
                     PSVGDRPARAFSRRDIAEILEPRVDEMFALVRREIVRAGYEGMLGAGVVITGGTSLLD
                     GMLDAAEKVLNLPARRGMPSGVGGLRDIVGHPSHSTGVGLLLHGRRHVDELETAGLRN
                     GGTWAKMRGWTKRVLEVF"
     gene            complement(7037..7894)
                     /locus_tag="VN039_15305"
     CDS             complement(7037..7894)
                     /locus_tag="VN039_15305"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWV47366.1"
                     /translation="MRFLGRKRRSRPAGPRKNQWKDPQGERAVKEAGRAKIARRKALV
                     RRVGLATGAGLVAWLVVMGMRYSGPALQTLLEIRTITVEGEHHIDKQKVLELAKVKPG
                     TGLHHIVTRVIKEQVESHPWVKEAEVTRVAFHELRISLVERIPAAVIRVDAQNFLTDA
                     EGHVLTKLGQIDDDALPLVTGIDSQGLLDGTESVRQVIASGIKLAKLVGQTFEGRLQV
                     NAENPSNLVALVQGMRFQFGEEAVGEQWERFRRVKPTLKTLNFDGHGRRANEVDLRYE
                     NRIVVREGG"
     gene            complement(7900..>8814)
                     /locus_tag="VN039_15310"
     CDS             complement(7900..>8814)
                     /locus_tag="VN039_15310"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWV47367.1"
                     /translation="ISLKTGQAVYQALIRRGYDAVAIDVTDRLHRDLEDHKVAIVFLS
                     LHGPGGEDGTVQGFLETLGIPYTGSGVRASAVGMHKAATKTILAAHGIPLPAGTVVRE
                     CDRPSLAKVLRQAKLKLPVVVKPVSQGSTIGVTVVRRAGQWREALDLAHRYDPEVMVE
                     SYIPGHEATVSILGTAAEGPTVLPAIEIVAPDGFYDFSAKYQKGRTRYLCPAPLPPKV
                     VHHIRELAGRTYEVLGCEGAVRIDFRITPRGRPYVLEVNTVPGMTETSLLPMAAAQAG
                     IAYDDLVEQILQSALDRASRFARVVSKE"
BASE COUNT         1907 a         2764 c         2209 g         1934 t
ORIGIN      
        1 aaacttcaag actccggatt cactcgcatg agaacgcggg ctaatttcaa ggggacacgc
       61 taccttgccg aaaaagaaac gtggctcgac tacccagacc ggtcttaaat cgagccgatg
      121 aattcctgat acaccaaatc atgaatcgca ggacggaatt cccggattgc ttcattctta
      181 ctcgaaggat gcactcgatt ggagagcaac acaacttcta attcttgcac tggatcgatc
      241 cagacggaag tccccgtata gccgagatga ccgaacgatc gcgctccgaa atatcgtccg
      301 gcagatgacg gcgttgatgg tgtatcccac ccaagcgccc aactggaaga tccttccggt
      361 ttctgacgcc gtgtaaactc ccgcgcaatt ccttgatcga ggatagcctt tcgaccatgg
      421 taagctttta accattcacc cgtaatagct aataccgcgt cggcagtccc aaacagtccg
      481 gcgtgaccgg cttcgccgcc caaagcggct gcattctgat cgtgcacttc tccgcacaag
      541 aggcgacctc gccatgggtc aacttcggtc ggtgccacgc caccgtcgtg cctgtttgca
      601 gattccagga actcgttcaa tcgctctcgc aagacgtatt caatccgcag cccgtccaat
      661 ggaaccgtga tgtaatcata aaaaaagtga tccagatcct gtcggctgca ccgctcgacg
      721 attatgccga gcaccatgaa accgaggtca ctgtacaggc tgcgcgtgcc ccgttcatac
      781 accggcgcct cagaccgaat gaggctgagc atagactgtt ttgcatggtc ccttgcttct
      841 ttagacgaag gaatctctcc ttcgggattg atccgctcat agtatggtcg ccatccgggt
      901 aatccggagg aatgagtcag taggtcccga agcgtggctg agccgatcga agtctctgca
      961 cattctggaa gatacttagc gacggtatct tcgagccgaa gatcaccctg tcgaatcagc
     1021 agggcaatgg acgtaacggt tgccaatggt ttggtcaacg aagccagatc atagattgtg
     1081 gaagtactga cgtcaggccc tggagggacg tccgaaagat ggccggcatg aaatcgcgaa
     1141 accggtctat ctccgcatcg taccgccaat acggctcccg ggaacactcc ctcggtaacg
     1201 gctcgattga gtgctgtttg aataacactg agatctggca tcgggatgat gatcgacgga
     1261 ggacgctccg gacaaacgat cgagcactac ctagcgcggt accatcaatc ggaaacagtc
     1321 aacgggtagg gctggattcc ccttccagtt tttcttcctg catgacacca gtatcttgat
     1381 agacttcact cgcctctata tccagcatcc gttcgaaggt atgaagcgtc gcccgcatcc
     1441 gctcaaccat gagcagccgc tgcttctgca gcacagacag gtctcgctgt gtgtttccca
     1501 gctcgactcg cgcttgtctg aacagttcgc cggctttcaa ctccgcttct ttgacgatca
     1561 actcggcatc gcgttgcgcg cttcgcttga catcctcggc caatgactgt gccgagacca
     1621 atgtggtaga caaagtcgcc tctgtccgtt tcaattccga gatctgctgc tcaagcgaca
     1681 caatgcggtc acgcaatatg gcattatcac ggttcaggaa ttcgacggtt tgtgcaatct
     1741 cttctaaaaa tcggttgacc tcttcacgat catagccacg aagcttgact tgaaagacca
     1801 tctgttgaat gtcgagcggt gtgatcttca tggcatcccc cattggttga atcaattcat
     1861 ccgtaccgcg atgtccttaa tcgactgtac gaccgcaatc tgtaaaaacc agatggccac
     1921 gatgacgacc aacggcgaca aatcgacgcc catgctccag cctaatcgcc gtctgatcgg
     1981 agccaagacc ggttcggtgg cacgactgag gaattgaacg atcggattcc atggatccgg
     2041 attcacccaa gaaatcaggg cccgagcgat gacgatccag gtatagaacg tcaatacata
     2101 gtccagtacc gtggcgaacc ctaacaaggt atttcctacc acaaacatca gcctccgagc
     2161 tcccgagaac gcttcgtcgc agactcaata gcgtcgatca acaggcctcg gagacctccc
     2221 tgctcaagcc gatgcaaccc ggcaattgtc gttcctccgg gggacgtcac ctgatccttg
     2281 agactcgcag gatgctgccc cgtttctaag accatccgtg ccgcacccaa cacggtctgc
     2341 gcggcgagaa gacttgacgt ttccctcggc aaacccatct tgacgccgcc atcggccatc
     2401 gcttcaatca tgagaaagac gtaggccggt ccgcttccgc tcaacccggt cacggcatcc
     2461 atgaagcgct cgtcgatggg cacaacccgg ccgaccgatt caaagatctg tcgtgcacat
     2521 gctatctcat tgtcccctac cccaggcccg atcgccagag cagtcatccc ttcgccgacc
     2581 attgccggcg tattcggcat cgcacgaatg acgcgagcct gcggcccgca agcctcaata
     2641 attcgaccga gtcgcactcc cgcgaccacc gagatcacga gccgtttcgt cagcacatcc
     2701 ccgatctctt tgaggacttc ggccgtcacc tgaggtttca cggccaggac gacgatatcc
     2761 cccaaggcga ccgcctcatg atttgtgaga ccaatctgaa tgccgtactg tctcttaaga
     2821 tggtccagtc tgaccgtatc tggatcagct acgtggatct ggtcggcctt gtactctctt
     2881 gcggaaagca ccccgctgat caatgcctcc gccatccggc caccacccac aaaggagata
     2941 ctttgtgagt gcgttgaact agacatcacg cgctccaaag atggcggtgc cgacacggac
     3001 cagtgtcgcc ccttcctcaa tggctacctc ataatcgttc gacattccca tcgacagttc
     3061 acccatccgc acggtcggta agtcgaatgc tgcaatctgt cgggcaagat cacggagtgt
     3121 gcggaaatac ggcctggccg aatccggatc agcagtcggc ggagggattg tcatgaggcc
     3181 tttgacacag acatgagaga gttgcgccat cattggtacc gatcgaacaa cgtcatcggg
     3241 atgaaatcct gcctttgtcg gttctttccc gatattcact tccagcaaga cattctgccg
     3301 acggccggcc tctcccgcac ggcgatcgat ctcttgcgcc aggtctagac tactcaccga
     3361 atgaatcagg tcgaagaggc cgatcacgga tcgtaccttc cttcgttgca gctgtccgat
     3421 aaaatgccaa tgaaccggtg gctgggtgaa ggcggctatt ttcggaagcg cttcctgcac
     3481 acgattttca cccaagatgg acaagccggc acgcacacct tccgcaatat gctcgacgga
     3541 cacggtcttc gtcgcggcca ccagtcgaac cgttccggca ggacgtccgg ctttttcttc
     3601 cgccgaccga attttcgtga gcaccgactg aacacgtttc gctatcgctt ccgtgatgag
     3661 cggttccatc gtcccctcgc acgagtcgcg gccgctcatt cagcgaaccg tccttattct
     3721 agcggaagaa cctccgaatt ggcagagtct gacctatcca cacctcaccg gcaacccgat
     3781 ggcgctgacc atggtgccgt tgactttcct ctctcgccga taggagaaaa agaggtcttc
     3841 atggcaaatc gtacagagat tgacggtcgt gatgaacttc ggagccgccc caagggcttg
     3901 cgcctgctct ttgacgagcg ctttcaaatc aagattcgct tttccgtctc ctctcgtccg
     3961 taccactttg tcccaatccg gaaattcctg acacagatgg tcgagaaccg gctcatccac
     4021 ttcatagcag caaaccccgg cggatgggcc gatactgatc cgtacgtgct ctaaacgcga
     4081 gccaaagcga gactccaaca gtgccagcgt tttggggaca atgccggcaa cagctccccg
     4141 ccagccggca tggacggcgg ccacgacatg ccgctttggg tcatgtatca gtacgggaac
     4201 acaatctgcc gtccgcaccg ccaccatgac cccaggttga tcagtgacca aggcatccca
     4261 gccgcccaca aatcggtccg tcgaagtcac ggtacggtcc accaccagcg cttccgttcc
     4321 atggacttgc tttactgaga gcgtccatga ggacgatgcc gtgcctgcga tccccttccg
     4381 cgggatcccc acttcgaggc caagacccat ggcatgccgg cgggtcccga aaaaatggcg
     4441 gacctgactc ccggtatccg caaacgccgg cacggtaatg accgatgttc ttgtcataat
     4501 caaggtcaat acctcatcac gtgaaaatat gcacgagatc agtctgtttg tttacgaaga
     4561 aatgtcggca catcccactc atcctcggca gtcaacaccg cccggtccat tgactctcgc
     4621 gcatcgttca ttcttctcaa aaaggtgggg cgatcgagat ctttcatcgg gcggtctacc
     4681 gccagtgacg cgcccatgcc ggccaacact gatggaacgg gtttggccgg ccgactcatt
     4741 cccctatcag ctccaatcgt ggcttcagct gagtcctctt ctcgttcaaa cccggtcgcg
     4801 atcaccgtaa tgatcagttc ttcgcccatc tctgggttga tgacctgacc gacaatgata
     4861 ttggcttcgg gatctgccgt ctgttggatg atggaggcgg cttcctcgat ctcgtgcagc
     4921 gacatgctgg ggcctcccgt aatattcagg aggacgccgc gagccccttc gacacttcct
     4981 tcctcaagaa gaggactgca catggccttt tgagcggctt cgatcgcccg atttggtcca
     5041 taggaaatgc ccatgcccat gaccgctcgt ccggtgtgtg acatcacggt gcggacatcg
     5101 gcaaaatcga cgttcacatg tccggtggtc gtaatcacgt cggcgatgcc ttggatcgct
     5161 tgccgcaaca cgtcatccgc gactttgaac gcttcgagaa gcggggtcga tttgtcgaca
     5221 atccccagca gccgctggtt tggaatgacc agcagtgtat cgacgtgccg acgcaaatcg
     5281 cgaattcctt cttccgcgtg cttgtttcgc cgtttgcctt catactgaaa tggtctcgtg
     5341 acgacgccta ccgtgaggat acccatttca cgggcgatgc tggccacaat gggggcggct
     5401 ccggtaccgg tccctcctcc catccctgcc gtgacgaaaa ccatgtccgc cccttcgaga
     5461 cattctcgga tgtgctcttt actctcaagc gcggcatctc tgccgatctc cggctttgcc
     5521 cccgcaccca acccgcgggt tcgttccggc ccgagttgga tcttataagg tgccaacgac
     5581 cgatcaagcg cctgtaggtc tgtgttactc gcgataaaat cgacacgcgc gagtccggag
     5641 gtgatcatcg tattgatcgc attgcacccg cctccgccga ttccgatcac cttgatgcgg
     5701 accggcgaca gtagatcttc ctgaaatgag aacattgggg cacctcctca catcgcctcg
     5761 cacgacggac ccacccaccg cccgcaaggg ttcaaaggtt aaaagacctc caacacccgt
     5821 ttcgtccacc cacgcatctt ggcccaggtt cccccattgc gaaggccagc cgtctccaat
     5881 tcatcgacat gtcgccggcc gtgtaacaac agaccgaccc cggtcgaatg actgggatgg
     5941 ccgacaatat cgcgtaatcc tccgacaccg gaaggcatgc ctcggcgcgc cggcaagttc
     6001 aagaccttct cagcggcatc gagcatgccg tccaataacg acgtgccgcc cgtaatcacg
     6061 acgccggcac ccaacatccc ttcatagccg gcgcgcacaa tttctcttcg gacgagcgca
     6121 aacatctcat caactcgcgg ctccaagatc tcggcaatat cccgtctgga gaatgcacga
     6181 gccggccgat ctccgaccga cggcacctcg acgatctgat gtccggtcac caactcagtc
     6241 cgcgcaacac catactgagt tttgattttc tcagcctcag tctgcgaggt aaggaggccg
     6301 atggctaaat ccttcgtcaa attttggccg ccgatgggaa ggaccgctga gtgacggatg
     6361 ctgccgtcca gaaaaatagc caggtccgtc gttcctcctc ccaagtccac cattgccacg
     6421 cctaagtcgc gctcctcttg actcaacacc gcctcgctgg acgccagcgg ctggagaatg
     6481 atgtcgacaa cgtcgagtcc ggctcgattc acgctcttca cgatgttctg agcggaggtc
     6541 acggcgccgg tgatgacatg cacattgact tccaaacgat tacccgacag acccaacggc
     6601 tcgcgcacac cttcctgccc atccaccata aactctcgcg gtaacacgtg aagaatcctg
     6661 cgttcgtggg ggatcacggc gagtgtccgc gcgctttcga tggcccgttg aatatcttcg
     6721 cgtgtcacct cggcccgctt cagtgctacg acacccttgc agttttcagc ggaaatgtga
     6781 ctgcccgcaa tgccggtgta gaccgagttg atctggacgg ccgccatcaa ctccgcttct
     6841 tcgaccgctt tcttgatgga ttcgacggtg ctttcaatat caacgacgac tcctttgcgc
     6901 aggccgcggg agggactcga tccgacgccg ataatgctga gccctccggc gtcggtcatc
     6961 tccgccacga tcgcacaaat cttggtggtt ccgatgtcga gtccgaccag aatttgatcc
     7021 cgcttcggca ccgcaatcac cccccttccc gtacgacgat tcgattttcg taccgaaggt
     7081 ccacttcgtt cgccctgcgt ccatgaccgt caaagttcag agttttgagg gttggtttga
     7141 cacgtcggaa ccgttcccac tgctctccga ccgcttcttc accgaactgg aatcgcattc
     7201 cctggacaag tgcgacgaga tttgacgggt tttccgcgtt cacttgcagc cggccttcga
     7261 atgtttgccc gaccaatttt gcaagcttga tgcccgacgc gatgacttgt ctcaccgatt
     7321 cagttccatc caacaagcct tgggaatcga ttccggtcac gagaggcagg gcgtcatcat
     7381 cgatctgacc aagcttggta agcacatgcc cctccgcatc agtcagaaag ttttgggcat
     7441 cgacacggat gacggcggca ggtattcgtt cgaccaggga tatgcgtaac tcgtgaaacg
     7501 cgacacgggt cacttcggcc tctttgaccc acggatgaga ttccacctgc tccttaataa
     7561 ctctcgtcac aatatggtga agtcctgtac caggtttcac ctttgcgagc tcgagcactt
     7621 tctgtttatc gatgtggtgc tctccttcga ctgtgatcgt cctaatctcc aataacgttt
     7681 ggagagcagg accggaatat cgcataccca tcacgacgag ccatgccacg agccccgcac
     7741 cggtcgccag tccgaccctt cgaacaaggg ctttccgtct ggctattttt gcccggccgg
     7801 cttccttcac tgccctctct ccttgcggat ccttccactg attctttcgt ggccccgctg
     7861 gacgagatcg tcgctttctt cccaagaatc gcataacact cactcctttg agaccacccg
     7921 tgcaaagcga ctcgcgcgat cgagcgccga ctgtaggatc tgttcgacca agtcgtcata
     7981 cgcaattccc gcttgggctg cggccatagg cagcagactc gtttccgtca tgccgggaac
     8041 cgtattgact tccaagacat acggccgacc tctcggagtg atgcgaaaat ctatgcgaac
     8101 ggctccctcg cagcccaaca cctcgtaggt tcgtccggct aactcacgga tgtggtgaac
     8161 caccttggga gggagcggag cgggacagag ataccgggtc ctgcccttct gatattttgc
     8221 cgagaaatcg taaaaaccat cgggcgccac gatctcaatc gccggaagta ctgtcggacc
     8281 ttccgccgcc gttcccagaa tggacactgt cgcttcatgt ccgggaatat aactctccac
     8341 catcacctct ggatcatagc gatgggccag gtcgagagcc tccctccatt ggccggctcg
     8401 gcgcacgaca gtgacgccga tcgtggagcc ctgtgaaacc ggcttgacga caaccggtaa
     8461 tttcagtttt gcctgcctta gaaccttcgc caatgacggc ctgtcgcatt cccgcacaac
     8521 ggttccggcc gggagcggaa taccatgcgc ggccaatatc gtttttgtgg ccgccttatg
     8581 cattcccacg gcacttgcgc gcacgccgga tccggtatag ggaattccca atgtttcgag
     8641 aaacccctga accgttccgt cttcaccgcc cgggccatgc aacgagagaa agacaatggc
     8701 gactttgtgg tcctccaaat cccgatgcag acggtcggtg acatcgatag ccaccgcgtc
     8761 gtatcctcga cggatcagcg cctgatagac ggcctgaccg gtcttcagcg aaat
//
LOCUS       DATMTI010000968         3751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_503470, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000968 DATMTI010000000
VERSION     DATMTI010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503470"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VN039_15315"
     CDS             complement(<1..793)
                     /locus_tag="VN039_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47368.1"
                     /translation="MDTELGLATGYIETFAGNGKARSTGDGKRAVKAGIPLPHHIALD
                     KTEQWLYFAESGSDRVRRINLQEGTVHNFAGIGETCYSGDEGLCGEAGLYLPLDVAFD
                     SQNNLYICDSGSNRIRKVDHVTGIITTVVGTGQHGFNGDGPALEVNLTWPAAIAFDQD
                     DVLFIADTQAHKVRRFDPKTGLVTTIAGTWSTEDEAREQPLVARNLVVLSGDAIGIDF
                     SDDQGWLMPVCSDGLDMSMYLDDGRSAIDARLYDIVGLAVDAGGDV"
     gene            1066..2085
                     /locus_tag="VN039_15320"
     CDS             1066..2085
                     /locus_tag="VN039_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWV47369.1"
                     /translation="MTITRMGIDLAKHVFQLHGVDERGYTVLRRRISRTQLCPFFAQL
                     GPCLVGIEACGSAHHWARELRALGHEVRLIAPQFVAPYRKNEKNDGNDAEAICEAVGR
                     PHMRFVPVKDVEQQAVLTVHRARQLLVTERTAVVNQTRGLLAEYGLIVPAGIGALRRA
                     WGTLLETPELPVLAREVFADLADRLRTLDGRIATYDRRLAQLARQTEPAQRLMRVPGV
                     GPVTATALVATVGNARSFKNGRQLAAWLGLVPRQHSSGGRQRLGRITKRGDVYLRTLL
                     IHGARAVLRQLAQRTDATSRWALALKARRGFNKTVVALAAKQARMLWVLLATGRSYQS
                     AGVYL"
     gene            complement(2356..3180)
                     /locus_tag="VN039_15325"
     CDS             complement(2356..3180)
                     /locus_tag="VN039_15325"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HWV47370.1"
                     /translation="MTYIDSSRSHLLGLLFVLIVVAAVFGAFGIPLVSSEGMTIRSYL
                     VQGDLPNAADDVAWKNVSPITIPLSGQVITRPVWPEPTVRALTVRSVHNGTEIAFLLE
                     WQDNTKNDRLTPGTFRDGVAIGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDRRA
                     ARGSEKKEGGVRTFEVIPRRVSSVEDLIGGGFSTLTTKERQGRVQGQAVWRDGVWHVM
                     MRRPLVSEEQENEATLVPGRVQTVAFAVWNGENKERNGQKAVAPWFQLAIDPVAKL"
     gene            complement(3268..>3751)
                     /locus_tag="VN039_15330"
     CDS             complement(3268..>3751)
                     /locus_tag="VN039_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47371.1"
                     /translation="VDAKGDVYVVDKGSNRVRKIDSRTGIISTVAGVCRYGYDGDEKP
                     AVRAMLHAPESVIFDHDENLYISDTMNHRVRKVDAKTGLIATVAGNGDSGYEDKNIGG
                     CGAARFVAKESAGMLRHGDGLLGTEAVVNSPVGLSVDSQGHLYICERGENKIRRLKLS
                     "
BASE COUNT          783 a         1132 c         1063 g          773 t
ORIGIN      
        1 acacatcgcc gccggcatca accgccaacc cgacaatgtc gtagaggcgc gcatcgatcg
       61 cagaccttcc atcgtccaga tacatcgaca tatccaaccc atccgagcag accggcatga
      121 gccatccctg atcatcgctg aaatcgattc cgatcgcgtc gcctgacagc acaactaaat
      181 tcctcgccac cagcggctgc tctcgcgctt catcttcggt cgaccaggtc cctgcaatcg
      241 tcgtgaccaa gcctgtcttc ggatcaaacc gtctgacttt gtgcgcttgg gtatcggcga
      301 taaagagcac gtcgtcttga tcaaaggcga tcgctgcagg ccaggtcaga ttgacttcca
      361 gcgcgggacc gtccccattg aatccatgct gaccggtacc gaccaccgtc gtaatgatgc
      421 ccgtgacatg atcgaccttt cgaatccggt tgctcccgga atcacagatg tacaaattgt
      481 tctgggaatc aaacgcgacg tccaggggca gatagagtcc tgcctcgcca cagagacctt
      541 catcgccgct gtagcaggtt tcaccgattc cggcaaaatt gtggacagtg ccttcttgaa
      601 gattgatgcg ccggactcgg tcagaccccg actcggcaaa atagagccac tgctccgtct
      661 tgtcgagcgc gatatgatgg gggagcggga ttccggcttt caccgcacgc ttgccgtcac
      721 ccgtgcttcg ggccttaccg tttccggcaa aggtttcaat gtaaccggta gccaatccga
      781 gttcggtatc cataggtacg atgctcgcta ggaggttatt aaaagattat tttgccgctc
      841 gcatacttcg actctcggcg caggatgttc aaaaagacct tccagcaagg ccgcagcgag
      901 cgaggaggcg aggcgtacgc ttcggtacgt tgagcctctc tgagcgatgc gagaacgccg
      961 ttggcggact ttttctaatg ggatggactc ccccgcttca gtaccatggg ctctaccgga
     1021 ccacggtgtg aacccacaac gaccagaaga agggagtcca cgatgataac catcactcgc
     1081 atgggaattg atctggccaa gcatgttttc cagctccacg gcgtcgatga gcgagggtac
     1141 acggtcctgc gccgccgaat ctcgcggacg caactgtgtc cattctttgc gcaactcggg
     1201 ccctgtctcg ttgggatcga agcctgcggc agcgcgcacc attgggcgcg ggagttgcgg
     1261 gcgctggggc atgaggtgcg gctgatcgca ccgcagttcg tggctccgta tcggaagaac
     1321 gagaagaacg atggtaacga cgccgaggca atctgtgaag cggttggccg cccccacatg
     1381 cgctttgtcc cggtgaagga cgtcgagcag caggcggtgt tgaccgtcca tcgggcccgg
     1441 cagctcctgg tgacggaacg gacggcggtg gtcaatcaga cgcggggctt gttggcggaa
     1501 tacgggttga tcgtacctgc cggtattggc gccttgcgcc gcgcgtgggg cacacttctg
     1561 gagacgcccg agctgccggt gttggcccgc gaggtatttg ccgatttggc cgatcgtttg
     1621 cgcaccttgg atggccggat cgccacgtac gatcgtcggc tggcgcagct ggctcgtcag
     1681 acggagcctg cccaacggct catgcgagtc ccgggcgtgg gaccggtgac ggctacagcc
     1741 ttggtggcga ccgtgggcaa tgcgcgcagt ttcaagaatg ggcgccaact ggctgcctgg
     1801 ttgggcctgg ttccgcgaca acattcgagt gggggccgcc aacggctggg acggatcacc
     1861 aaacgcggcg atgtctatct tcgtacgttg ttgattcacg gtgcccgggc ggtgctgcgg
     1921 caactcgcgc aacgcaccga tgcaaccagt cgctgggccc tggccctcaa agctcggcgc
     1981 ggcttcaata aaacggtggt ggcgctggcc gccaagcagg cgcggatgct ctgggtgctg
     2041 ctggcaacgg gacggtccta tcagtccgct ggcgtgtacc tctgagccaa cgaggtgcgt
     2101 gaaagattgc tcgtcatgac gacatgcgag gctcaggagt tgatggcatg accggtcgga
     2161 ccggcgccgg gtgaccctgc tagcaggact ggccgtcaga ggccgttttt ctgatgagga
     2221 cccggtgcgc gaatatccat cagggcccga acccaaagat gggttcacca caaggccgaa
     2281 tatatgcgtg cagtcagacc tcattgccat gactcgtgac cttgcatcga agggggagtc
     2341 catataaaca gcctgctaga gtttggcgac gggatcgatc gccagttgaa accatggcgc
     2401 cacagccttc tggccgtttc gctctttgtt ttctccgttc cacacggcaa atgcaacggt
     2461 ctgcacgcga ccaggaacga gcgtagcttc attctcttgc tcttcactca ccaagggacg
     2521 acgcatcatg acatgccaaa caccgtccct ccataccgcc tgtccctgca ctcttccctg
     2581 cctctccttt gtggtcaacg tgctgaatcc cccgccgatt aaatcttcca ccgaagagac
     2641 tcggcgcggg atgacttcga aggtccgaac ccctccctct tttttctccg atccccgagc
     2701 cgcccgtcga tcgatgtcgc tttgccaatc cgccttccaa tgccagatgt tgatatagtg
     2761 gtcgagctgc cccatgcaga aaaatgccgg cgcatcgccg agcggaaggc cgatcgcaac
     2821 cccatcccgg aaggtgccgg gagtcagccg atcattcttc gtattgtcct gccattccag
     2881 cagaaaggcg atctcggtcc cgttgtgaac ggaccgcacc gtcaacgcac gcacggtggg
     2941 ctccggccat acaggtctcg tgatcacttg cccgctcaag gggatagtga tcggggagac
     3001 attcttccaa gcgacatcat cggcggcgtt cggcaaatca ccttgaacca agtacgatct
     3061 gatggtcatt ccctccgaac tgaccagtgg aatcccaaac gctccgaaca cagcggcaac
     3121 gacaataagc acgaacaaca acccaaggag atgagagcgg gaactgtcga tgtacgtcat
     3181 gcgcaccgca caatactccg aaagaaagcc gaattcagaa tatcacaaaa cgcgtgcgag
     3241 acaaatgtag aggcaagggg gtattgacta cgagagcttc agacggcgga tcttattttc
     3301 tcctcgttcg cagatataga ggtggccttg cgaatccacg gacaaaccga cgggtgaatt
     3361 caccacagcc tcggtcccaa gcaggccgtc tccatgtctc aacattccgg ccgattcctt
     3421 ggcgacgaat cgagcggctc cgcagccgcc gatgttcttg tcttcatatc cactgtcccc
     3481 gtttcccgca accgtggcaa tgagaccggt cttcgcatcc actttacgga cccgatgatt
     3541 catggtatcg gaaatgtaga gattctcatc atgatcaaaa atcacggatt ccggggcgtg
     3601 caacatggct ctgacggccg gcttctcgtc gccgtcgtat ccgtaccggc agactcctgc
     3661 gacggtcgaa ataattccgg tccgggaatc gatctttctg acgcgattgc tgcccttgtc
     3721 gacaacatac acgtcaccct tggcatcaac c
//
LOCUS       DATMTI010000969         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_206888, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000969 DATMTI010000000
VERSION     DATMTI010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206888"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..>1787)
                     /locus_tag="VN039_15335"
     rRNA            complement(12..>1787)
                     /locus_tag="VN039_15335"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          389 a          559 c          382 g          457 t
ORIGIN      
        1 atattacctg ctatgagaaa gcttcgggca attagtatta ctcggctttg atgtcaccac
       61 ctttatacct gtaacctatc aacgtagtag tctgctacga ccctcaatgg aagtctcatc
      121 ttgtggctag tttcgcactt agatgctttc agcgcttatc tattccccac atagctaccc
      181 ggccatgcta ctggcgtaac aaccggctca ccagcggtgg gtccaacccg gtcctctcgt
      241 actaaggtca gctccactca aaattcctac gcccacaaca gatagggacc gaactgtctc
      301 gcgacgttct gaacccagct cgcgtgccac tttaatgggc gaacagccca acccttggga
      361 ccttctccag ccccaggatg tgacgagccg acatcgaggt gccaaacctc cccgtcgata
      421 tgagctcttg ggggagatca gcctgttatc cccagcgtac cttttatcct ttgagcgatg
      481 gcccttccat acagaaccac cggatcacta tgtccgtctt tcgaccctgt tcgacttgtc
      541 ggtctcacag tcaagcaagc ttatgccatt gcactccgcg tacggttacc aagcgtactg
      601 agcttacctt tgaaagcctc cgttaccttt ttggaggcga ccaccccagt caaactaccc
      661 accaaacaat gtcctccaca tgatggagtt agaaaccgga tacagaaagg gcggtatttc
      721 aagggtgatt ccatgactcc tggcgaagcc acttcaatat ctcccgccta tcctacacat
      781 cctgtaccca atctcaatgt taagctatag tgaaggtgca tggggtcttt ccgtcccgtt
      841 gcgggtaatc ggcgtcttca ccgataccac aatttcaccg agctcatggc tgagacagcg
      901 cccagatcgt tacaccattc gtgcaggtcg gaacttaccc gacaaggaat ttcgctacct
      961 taggaccgtt atagttacgg ccgccgttta ctggggcttc gatcaagagc ttcgccttgc
     1021 ggctaacccc atcaattaac cttccagcac cgggcaggcg tcagacccta tacgtccact
     1081 ttcgtgtttg cagagtcctg tgtttttgat aaacagtcgc aaccccctct tcactgcggc
     1141 cctcttcggc tttaacacct aatgagggcg tgccttctcc cgaagttacg gcactagttt
     1201 gccgagttcc ttcatccgag ttgtctcaaa cgccttagga tactctcctc gcccacctgt
     1261 gttggtttgg ggtacggatt cctgttgcct gaagcttaga agcttttcct gggagcaggg
     1321 tatcaatcgc ttcagcccaa aagggcctcg tcatcacatt tcagaattga ccccccggat
     1381 ttgcctaagg gaccttccta cgtgcttaaa ccagcatcca acagccggcc gacctaacct
     1441 tctccgtcac tccatcgcag caacagcaag tgcaggaata ttaacctgct ttccatcgac
     1501 tacgcctctc ggcctcgcct taggatccga ctcacccagc gccgaatatc gttgcgctgg
     1561 aaaccttggg cttacggcgt gacggttttt caccgtcatt atcgttactc atgtcagcat
     1621 tcgcacttct gatacctcca gcagacctta cgatccacct tcgcaggctt acagaacgct
     1681 cctctaccac gcatattgct atgcatccgc agcttcggta ctcagcttga gccccgttac
     1741 atcttccgcg cgggccgact cgaccagtga gctattacgc tttcttt
//
LOCUS       DATMTI010000970         1970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_77902, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000970 DATMTI010000000
VERSION     DATMTI010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77902"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..677
                     /locus_tag="VN039_15340"
     CDS             360..677
                     /locus_tag="VN039_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47372.1"
                     /translation="MKTTLPSNRMFCAVLLLFAASLVAVSAQAADSPPATRAPGFDTT
                     TEPRIADGAVEDTLKACMARIPKDASIGQRMIAEQSCGRDESDRKPFLAVPGARSVRH
                     EQP"
     gene            874..1353
                     /locus_tag="VN039_15345"
     CDS             874..1353
                     /locus_tag="VN039_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47373.1"
                     /translation="MIWFVAGAGTMVLLYYAASLSHAWKASGRVAASRSTGRTRLAAV
                     SLDTHIATEWTDGRIRLEALLALIETLAASLEREVATLNRLDTSDPLYERAARNMVSP
                     ALRRFTFDQHLRDECRVLVPLLEFCGRDLAESQGMNTTEQLAALAEIKAAIQSVDRG"
     gene            1477..>1970
                     /locus_tag="VN039_15350"
     CDS             1477..>1970
                     /locus_tag="VN039_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47374.1"
                     /translation="MMQHQLFVVAFFLVLLVLFYQIAVIFAPFFVPVLWAVIIARMSN
                     GPYLKLVHLLRGRDTLAAALLTFATMFLVVFPILSLTFLLVQETTIAYRSVVEWVQAD
                     GMKQLPEYLGKIPLAGRFIQEQLGTFVLAGSGYEGSIAEGTKTVGTFLLTHISGLARD
                     AFSVT"
BASE COUNT          397 a          539 c          593 g          441 t
ORIGIN      
        1 ggcttctgtg atgatcacga aggcctggca acaaatccac ttctagctga cacatcttcc
       61 agtgtcagca tgacagaggt cgccacgccc gcgctccaat gtgccgacga ttcatattct
      121 atgtggaggc gacataccac atttacatca caaaccgtgg ggacaagatc aagatggaga
      181 aactctcatg aggtcgggga gctaccacgc aagctcagcg gaccaggcgc tgttctgttt
      241 tgaccagact gcgaataggt ttcggaggat actgtagcct ggctcaacag atgtgcggca
      301 tcgtcagcgg accagagggc agtatcttgg ctatgttcct atcgaaggag gaatgcacaa
      361 tgaaaacgac tctacctagc aatcggatgt tctgtgcggt gctcctcctg ttcgctgcct
      421 cgttggtcgc agtctcggcc caggcggcag actcgccacc ggcaacgcgt gcgcctggat
      481 tcgacacaac caccgagccg agaattgcag acggggcggt tgaagatacg ttgaaggcct
      541 gtatggccag gataccgaag gacgccagta tcggccagcg tatgatcgct gaacagagtt
      601 gtggacggga tgaaagcgat cggaaaccct tcctggccgt ccccggcgcg aggagcgtac
      661 gtcatgaaca gccgtgatca agcttggtag gagatcggca tctgagagcc ttcggcaaag
      721 gcggggccct gagattttga gatgccctgt gcatcagtac ctgggtatct gtaggacgga
      781 ccgaactcgt gcttggactt ccggccaggg ttactcgatc gccgtcaggg tcgtggaaga
      841 ggcgttatgc agaatgtagg gatcacttca acgatgatct ggtttgtggc gggggccgga
      901 accatggtgt tgttgtatta tgcggccagt ctctcccatg cgtggaaggc gagcggacgc
      961 gttgccgcaa gccgctcgac cggccggacg aggctcgcgg cggtatccct tgacacgcat
     1021 atcgccactg aatggaccga cggccgcatc cggttggagg cgttactggc attgattgag
     1081 actctcgcgg cgtcgctgga gcgggaggtg gcaacactaa accgccttga tacctccgat
     1141 ccattgtatg aacgggccgc gcgcaatatg gtcagccctg ccttgcgtcg tttcaccttc
     1201 gaccaacatc tgcgggatga gtgccgcgtg ttggtccccc tcctggagtt ctgtggcagg
     1261 gatctcgctg aatctcaggg gatgaacacc accgaacagt tagcggcgct tgccgaaatc
     1321 aaggcggcga tacagtccgt cgatcgcggc tgattcctgg aaacgtacac gatcctgatg
     1381 ggtctgccga tctgtcccaa cctgccgacc gcaagctggg tcgggataca ttctggatct
     1441 accgcttcag aaaccggcct tgagcaggga aagtcaatga tgcagcatca actgtttgtg
     1501 gtggcgttct tcctcgtcct ccttgtgctt ttctaccaaa ttgccgtgat ctttgcgccc
     1561 ttttttgtcc ccgtcctgtg ggccgtcatc atcgcccgca tgagcaatgg cccgtacttg
     1621 aaattggtcc atcttctgag ggggcgagac acactggcgg ccgctctgct gacatttgcg
     1681 accatgttcc tcgtcgtctt cccgatcctg tctctgacct ttctgctcgt gcaggaaaca
     1741 acgatcgcct accggagtgt cgtggaatgg gtccaggccg acgggatgaa acagctcccg
     1801 gagtacctcg gcaagattcc gctggctggg cggtttatcc aggaacagct ggggactttc
     1861 gtgctggcgg gaagtgggta cgaaggctcg atcgcggagg ggacgaaaac agtgggcacg
     1921 ttcttgttga ctcacatctc tggtcttgct cgtgatgcct tttctgtgac
//
LOCUS       DATMTI010000971         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_714449, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000971 DATMTI010000000
VERSION     DATMTI010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714449"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VN039_15355"
     CDS             <1..1174
                     /locus_tag="VN039_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWV47375.1"
                     /translation="GIDDPNTFRLMAILLNQTAHQALLDNRNILVVDDEEPIRRLLAY
                     LLQSHGYPVECAADAREARQKLRDQPFALMLCDVNMPGESGMDLVRYILKEHTHTAAI
                     MVTGLDSSVLANAALEVGAFGYIIKPFESNEVLIDVANALRRRKLEMENRLHRENLED
                     IVRTRTLALQQALDWLERTEKELRLSREETIQRLAIAAEFRDHATARHIQRMSHYCEL
                     LARKAGLSPERCDLIRTASPMHDIGKIGTPDHVLLKPGKFTQEEFGVIAQHAEIGYKI
                     LSGSDAELLKVAAVIAYTHHERFDGTGYPRRLQGEAIPIEGRIAAIADAFDALTTQRV
                     YKPAFELSHAIELMLKHRSEHFDPELLDVFVASTDEVARIHDQYADRVNSNTLRET"
     gene            1313..1816
                     /locus_tag="VN039_15360"
     CDS             1313..1816
                     /locus_tag="VN039_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47376.1"
                     /translation="MAKKLAAVRKRPRNRNWIAYLCESLVASGTMPTWEAATYLTENL
                     FGEKPNPRLLTPANPHEVTAQFLHRLYQPLVEAASREVMLSSSSMLHIFTDISLTGNS
                     AVLVVLFPGHNKPHQLLMLDAAKAWDLVFPDAESFNAWAEERYRQLVNVLRSKMTEHE
                     DDLLTAI"
     gene            complement(2081..>3224)
                     /locus_tag="VN039_15365"
     CDS             complement(2081..>3224)
                     /locus_tag="VN039_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWV47377.1"
                     /translation="EWHGKSIYVLNRHNGKLIQEIDLVDGPEVKNPKFGGKTCHDPAH
                     VMTRVDTQQVHVGCNGDDMVVEFNRDPVSGLLSIHQFISMSKNPGVDKQAQPHAHWMG
                     FDGKSMVTPNSNTGDSTLFNFNEYKIVEKQPTGTLPIATGIMPDSSKYYVSNYLGDTI
                     SVLCGPYPKTGCVPGTVIKTLNLLDDNADNSGYITNGRFLDPTTPAANLPGPAGGFSV
                     QTPVSPDGKFVVIGNTLSGTITIIDTATDTLVRSIPGDPGCHGVNFGAKKGGGYYAYV
                     TSKFANRLIVLDPDPNNDGKADDAVIVGAVVLADDHVAKDDRITGNRGMGGQGVMPVP
                     TVYNGWVQELPIAYKAQLTKAQRNPLGKKGHQHHDDDDDHDHGDDR"
BASE COUNT          711 a          979 c          844 g          690 t
ORIGIN      
        1 cggcatcgac gatccgaata cattccgact catggcgata ctgctgaacc agaccgctca
       61 tcaagctctg ctcgacaatc gcaatattct ggtggttgac gacgaggaac cgatcaggcg
      121 ccttctggcc tatctgcttc aatcccatgg gtatccggtc gaatgcgccg cagatgcccg
      181 agaggccagg caaaagctgc gtgaccagcc ctttgcattg atgctctgcg atgtcaacat
      241 gccgggagaa tccggtatgg atctcgtgcg atacattctc aaggagcaca cacacacggc
      301 ggcgatcatg gtcaccggcc tcgatagctc ggttctcgcc aatgccgcct tggaggtcgg
      361 tgcgttcgga tacatcatca agccgttcga atccaatgaa gtgctcatcg acgtcgccaa
      421 tgccctccga cgtcgaaagc ttgagatgga aaatcgcctc catcgtgaaa atctggagga
      481 catcgtccga acaagaacgc tcgcgttaca gcaggcgttg gactggctgg agcgcaccga
      541 aaaagagctg cgcctgtcgc gtgaggaaac cattcaacgg cttgccattg ccgcggagtt
      601 ccgcgatcat gcgaccgccc gacacattca acgcatgagc cattactgtg aattgctcgc
      661 gcgcaaagcc ggcctatctc ctgaacgatg cgacttgatc cgcacggcca gccccatgca
      721 cgacatcggc aagatcggca ccccggatca tgtgttgttg aaacccggca aatttacaca
      781 ggaggaattc ggggtgatcg cgcagcacgc cgaaatcgga tacaagatcc tcagcgggtc
      841 ggacgcggag ctgctgaaag tcgcggccgt gatcgcctac acccatcatg agcgcttcga
      901 cggcaccggc tatccccgcc gattacaagg cgaggccatt cccatcgaag gccgcattgc
      961 cgcgatcgcc gatgcatttg acgcactcac cactcaacgg gtctacaaac cggctttcga
     1021 gctcagtcat gcgattgaac tcatgctcaa gcaccgtagc gaacacttcg acccggagct
     1081 gctggatgtc ttcgtcgcct cgacggatga agtcgcacgc atccacgatc aatacgcaga
     1141 ccgcgtgaat tccaataccc tccgcgaaac atagcctctc ctcagccgaa cccattgcgc
     1201 gattgtcggc aattcgttga ccgactgcgg tgccgccgat atactttttt cacctgccgg
     1261 aacaacctga ttcgctccca accaccgggg ccataaaaag ggattgccga gtatggcgaa
     1321 aaagttggct gcggttcgca aacgtccccg aaaccgaaat tggatcgcct acctctgcga
     1381 atccctggtt gcttcaggca ccatgccgac atgggaggcg gctacgtatc tgacagagaa
     1441 cctatttgga gaaaaaccaa atcctcgcct cctgactccc gccaacccgc atgaggtcac
     1501 tgcgcaattc ctgcatcgcc tttatcaacc gctcgtcgaa gccgcctcgc gggaagtcat
     1561 gctctcttct tcctccatgc tgcatatctt caccgacatc agcctcaccg gtaattcggc
     1621 cgtcctcgtc gtcttgttcc ccggacacaa caagcctcat cagctcttga tgctggacgc
     1681 agccaaggca tgggatctcg tgttcccgga cgccgagtcg tttaatgctt gggccgagga
     1741 acggtacaga cagctcgtga atgtcctgcg cagcaagatg acggaacacg aggacgatct
     1801 gctgacagcg atctaaacag cctactcagg atcgtagttg aaattcggca agaaccggcg
     1861 ctcgattaat ccttctgatt tctgggaccg tgccagactg ccaccgggac gcgagagagg
     1921 gatgccgcga catcgcccgg caagacagac accgtggagg cggtgtctgc tcctcctggt
     1981 tcaatcatga tgaccgctgt tgtggcgtcc tggtgaacgt gataaagcca agagagacgc
     2041 agatatcatc tcatggctga ccgccctagg aacgccgatc ttaacgatca tcaccatggt
     2101 catggtcgtc atcatcatcg tgatgctgat gacctttctt gcccagcggg ttgcgctgag
     2161 ctttcgtcag ctgggccttg taggcaatcg gcagttcctg aacccagccg ttatagacag
     2221 tggggaccgg catcacccct tggccaccca tgcccctgtt tccagtgatc ctgtcatcct
     2281 tcgccacgtg atcatccgcc aacacaaccg cgccgacaat caccgcatca tcggccttgc
     2341 cgtcgttgtt cggatccgga tccaacacga tcaatcggtt ggcaaacttc gaagtgacat
     2401 aagcatagta cccgccgcct ttcttggcgc cgaagttcac cccatggcag cctggatcac
     2461 cggggatact tctcaccaag gtgtcggtcg cggtgtcgat aatggtaatc gtccccgaca
     2521 gggtgttccc gatcaccaca aacttcccgt ccggactcac cggcgtctgg accgagaacc
     2581 cgccggccgg accggggagg ttggccgccg gcgtcgtcgg atcaagaaaa cgcccgttgg
     2641 tgatatagcc gctattgtcg gcattgtcat cgagcaggtt gagcgtcttg atcacggttc
     2701 ccggaacgca gcccgttttg ggataggggc cgcacagcac cgagatcgtg tcgcccaagt
     2761 agttggacac atagtatttg ctcgaatccg gcatgatgcc cgtcgcgatg ggcaacgtcc
     2821 cggtgggctg tttctccacg atcttatact cattgaaatt aaacagcgtt gagtcgcccg
     2881 tattcgagtt gggggtgacc atcgacttcc catcaaatcc catccagtgg gcatggggct
     2941 gagcctgttt gtcgactcca ggattcttag acatggagat aaactgatgg atgctgagta
     3001 ggccggaaac gggatctcga ttaaattcca ccaccatatc atcgccgttg caccccacat
     3061 gcacttgctg cgtatccact cgcgtcatca cgtgagcggg atcatggcat gttttcccac
     3121 cgaatttcgg attcttgact tctggtccgt cgacgagatc aatctcctga atgagctttc
     3181 cattatgacg gttgagcacg tagatgctct tgccgtgcca ttcc
//
LOCUS       DATMTI010000972         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_757499, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000972 DATMTI010000000
VERSION     DATMTI010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757499"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1664)
                     /locus_tag="VN039_15370"
     rRNA            complement(111..1664)
                     /locus_tag="VN039_15370"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          365 a          525 c          383 g          455 t
ORIGIN      
        1 gaagcaaagc tacttcgaaa gcatatcccc ctttgcatcc gcaaagggat cggcgacttt
       61 cgtcagcctt ttttttgaat gtcttcgttg cagaagacga ttctccatag aaaggaggtg
      121 atccagccgc accttccgat acggctacct tgttacgact tcaccccaat tatctatccc
      181 accttagacg gctggctcct aaaaggttac ctcaccggct tcgggtgtta caaactctcg
      241 tggtgtgacg ggcggtgtgt acaagacccg ggaacgtatt caccgcggca tgctgatccg
      301 cgattactag caattccgac ttcatgcagg cgagttgcag cctgcaatcc gaactgagac
      361 cgcctttctc gggattcgct ccacctcgcg gctttgctgc cctttgtacc gtccattgta
      421 gcacgtgtgt agcccaactc ataaggggca tgatgatttg acgtcatccc caccttcctc
      481 cggtttgtca ccggcagtca tcctagagtg cccgacctta ctcgctggca actagcatca
      541 agggttgcgc tcgttgcggg acttaaccca acatctcacg acacgagctg acgacaacca
      601 tgcaccacct gtctcccctg tcccgaagga aaggcacatc tctgcaccgg tcagggggat
      661 gtcaagacct ggtaaggttc ttcgcgttgc ttcgaattaa accacatact ccactgcttg
      721 tgcgggtccc cgtcaattcc tttgagtttc actcttgcga gcgtactccc caggcggagt
      781 gcttactgtg ttaacttcgg caccaagggt atcgaaaccc ctaacaccta gcattcatcg
      841 tttacggcgt ggactaccag ggtatctaat cctgtttgct ccccacgctt tcgcgcctca
      901 gcgtcagtta cagaccaaag agccgccttc gccactggtg ttcctccaca tctctacgca
      961 tttcaccgct acacgtggaa ttccgctttt ctcttctgca ctcaagtctc ccagtttcca
     1021 atgaccctcc acggttgagc cgtgggcttt cacatcagac ttaagaaacc gcctgcgcgc
     1081 gctttacgcc caataattcc ggataacgct tgccacctac gtattaccgc ggctgctggc
     1141 acgtagttag ccgtggcttt ctggttaggt accgtcaagg tacaagcagt tactcttgta
     1201 cttgttcttc cctaacaaca gagttttacg acccgaaagc cttcatcact cacgcggcgt
     1261 tgctccgtca gactttcgtc cattgcggaa gattccctac tgctgcctcc cgtaggagtc
     1321 tgggccgtgt ctcagtccca gtgtggccga tcaccctctc aggtcggcta tgcatcgttg
     1381 ccttggtgag ccgttacctc accaactagc taatgcaccg cgggcccatc tgtaagtgat
     1441 agccgaaacc atctttcaat catctcccat gaaggagaag atcctatccg gtattagctt
     1501 cggtttcccg aagttatccc agtcttacag gcaggttgcc cacgtgttac tcacccgtcc
     1561 gccgctaacg tcatagaagc aagcttctaa tcagttcgct cgacttgcat gtattaggca
     1621 cgccgccagc gttcatcctg agccaggatc aaactctcca ataaagttag tttgtctagc
     1681 atctaaaata aaaattgacg tttcacgttg tttgtttcgt tcagtttt
//
LOCUS       DATMTI010000973         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_249823, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000973 DATMTI010000000
VERSION     DATMTI010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249823"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VN039_15375"
     CDS             <1..1341
                     /locus_tag="VN039_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47378.1"
                     /translation="IDGAQLPVGELAKTADKEPESETDKAATGLIDDAKLAAPAAAMT
                     KKPRAKSGAKVPNFNTFRKKLILGIGGGVLLILFLLWATLIAPRATVIITARTSDTAI
                     SQTVSLSQTAATDPAKNILHVVQQQQKDTQSVQFDATGTKNVGDKATGTVEFANNNAS
                     AMTIAQGTQLKSSSGLVFTTDAAVTVPAATLTFSMDCGSDHLCPGTATTGVTAAEGGT
                     DYNGASGSLSGAPNGVAVSFTDATTGGTDKTAKVVTAEDVKNATEELNGKVKENDEVK
                     AQLKNQFSSSVVPIEASFSANKSKPKSSPAVGGEANGQATLSIDVTYTMLGVDKSELS
                     SYLDVAAKSGIDEVSDQRVYDNGKDSATFSEYHSRSGADSAQLTATAKLGPDIKDDEI
                     KEKVKGKQYGDIQADIGAINGVDNVDTKFWPFWVNAVPNDTKRITVEFKLNDQK"
     gene            1328..>1633
                     /locus_tag="VN039_15380"
     CDS             1328..>1633
                     /locus_tag="VN039_15380"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HWV47379.1"
                     /translation="MTKSKSFLCLDVGAARIGVAVADSEVKLAVPFGFVPVDGEEIKE
                     IARLVLLQGADTLVVGYPRNQSGEPTAQTAYVEAFTEQLKDIVSDIVFQDESLTSVLA
                     "
BASE COUNT          420 a          415 c          478 g          320 t
ORIGIN      
        1 atcgacggag cacaactgcc ggtcggtgaa cttgccaaga cggcggacaa agaaccggaa
       61 agcgaaaccg acaaggcagc gaccggtttg attgatgatg ccaaactggc ggcaccggcc
      121 gcagcgatga cgaagaaacc gagggccaag tccggcgcga aagtgccgaa tttcaatacg
      181 ttccgcaaga agcttatcct gggtattggc ggtggtgtcc tgctcatcct attcttgcta
      241 tgggcaacgc tgatcgcacc ccgtgctacg gtcatcatta cggcccggac gtcagatacg
      301 gcaatcagtc agacggtcag cctgagtcag accgccgcga ccgatccggc taaaaacata
      361 ctgcacgttg ttcagcagca gcaaaaagat acgcagtcgg ttcaattcga cgctactggc
      421 accaagaacg tcggtgataa ggccactggc acggtggagt ttgcgaataa taacgccagc
      481 gccatgacaa tcgctcaagg gacgcagctg aagagcagta gcggtctcgt gtttacgact
      541 gacgcggcgg taacggttcc agccgcaacg ctcactttta gtatggactg tggttccgat
      601 catctttgtc caggaactgc cacgacgggt gtcacggccg ccgaaggtgg aacggactac
      661 aatggcgctt ccggatccct ttcgggtgcg ccgaatgggg ttgcagttag cttcactgac
      721 gctacaaccg gtggtaccga caagacggcc aaagtagtta cagctgagga cgttaaaaat
      781 gctacggaag agctgaacgg caaggtcaaa gagaatgatg aggtcaaagc gcagcttaag
      841 aatcagttca gtagcagtgt cgttccgatt gaggccagtt ttagcgcaaa taaatcaaaa
      901 ccgaaatcca gtccggcagt cggtggtgag gcgaacggcc aggccacgct cagtatcgac
      961 gtgacctaca ctatgcttgg cgttgataaa tcggagctct cgtcctatct ggatgtggcc
     1021 gcaaagtcgg gcatcgacga agtcagtgac cagcgggtgt acgataacgg caaagactcg
     1081 gcgacattca gcgaatacca cagccgatct ggcgcggata gcgctcaact gacggcgacg
     1141 gccaagttgg ggcctgatat caaagacgat gagatcaaag aaaaagtcaa aggtaagcag
     1201 tacggtgata tccaggcgga tatcggcgcg atcaacgggg tcgataatgt cgatacgaag
     1261 ttctggccat tctgggtaaa cgcggttccg aacgatacga agcgcattac cgtcgagttt
     1321 aagctcaatg accaaaagta aatcatttct ctgccttgat gtcggtgcgg cgagaatcgg
     1381 tgtcgcggtg gctgacagtg aagtgaagct ggccgtccca ttcggttttg tgccggtcga
     1441 cggcgaagaa ataaaagaga tcgcccggct tgttctcttg caaggtgccg acacgctcgt
     1501 cgtcggatat cctcgcaacc agtcgggaga gccgaccgcc cagacggcat atgttgaagc
     1561 gttcacggag cagcttaaag acatcgtctc cgatatcgtg ttccaagatg agtcactgac
     1621 gagcgtcttg gcc
//
LOCUS       DATMTI010000974         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_843664, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000974 DATMTI010000000
VERSION     DATMTI010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843664"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..389)
                     /locus_tag="VN039_15385"
     CDS             complement(162..389)
                     /locus_tag="VN039_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47380.1"
                     /translation="MSTAPALNPPRLGDPINDEDSYVKRGGAVTQAAHWNAVLADDDV
                     FGHGCGLLVGVSPVRDRAGRYPLVWAAATDL"
     gene            complement(386..610)
                     /locus_tag="VN039_15390"
     CDS             complement(386..610)
                     /locus_tag="VN039_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47381.1"
                     /translation="MASKKYSEVELDRIDIDLIRAGDKLRSARTKEQAAYQEAHRAAL
                     AALQAGFPETGVAEALGVDRMTVRKWAGKR"
     gene            complement(968..1043)
                     /locus_tag="VN039_15395"
     tRNA            complement(968..1043)
                     /locus_tag="VN039_15395"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1127..1447
                     /locus_tag="VN039_15400"
     CDS             1127..1447
                     /locus_tag="VN039_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47382.1"
                     /translation="MDSGSSQITEVATDVGIFKLKINAEGCWRITDPEDKLVAPNTPT
                     GRKLIAAAEGSEHVCELPSLEGLYPGDVWTCPCGAGWEVDEVRTADDEYFDWTPRLSS
                     GGTL"
     gene            1444..1728
                     /locus_tag="VN039_15405"
     CDS             1444..1728
                     /locus_tag="VN039_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47383.1"
                     /translation="MSDALYIIRSGDSTLGEVMTHEEVVRWVGVAISYRLEKTLCLTL
                     QSRALNAAAEAAVHWLSESLAKVPINQETARAFESVLRQHSEVFRQLENK"
     gene            1995..2333
                     /locus_tag="VN039_15410"
     CDS             1995..2333
                     /locus_tag="VN039_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47384.1"
                     /translation="MSGGFTDAVVTLLILAAFVAATFFICAVCVRWLIGYDAPARLWR
                     RRRIWRDGDCRVNINFGTPETGHWCDRCMKPSVYVMPLTALRPDGVKDLGAQAVKCLD
                     CGHGRIEETP"
BASE COUNT          542 a          701 c          788 g          584 t
ORIGIN      
        1 gtctgtcggg tcgaacccaa agcagtcgca catcgtgcag gccatcgttg ggccgtgtga
       61 atcccattgc tctagacgta gttggccgcc accggcaggg caccgaccct tagctagttc
      121 ttccgcctcg aaagccgcgt agtccgtgta cgggtcgctg ctcataggtc tgttgccgcc
      181 gcccacacca gcggataccg gcctgcacgg tctcttaccg gcgatactcc gacaagcagc
      241 ccgcaaccat gtccgaaaac gtcgtcatca gctaagacgg cattccagtg cgcagcctgg
      301 gtgacagcac caccacgttt gacgtagctg tcctcatcgt tgatcggatc acctagccga
      361 ggcggattca atgccggtgc tgtgctcatc gtttccctgc ccatttccgt acggtcatgc
      421 ggtccacgcc gagtgcttca gcgacaccgg tttcagggaa gcccgcttgg agtgcggcaa
      481 gggcggcgcg gtgtgcttcc tggtatgcgg cttgctcttt cgtccgtgcg ctgcggagct
      541 tgtctcctgc gcggatgagg tcgatgtcga tgcggtctag ctcgacttcg ctgtacttct
      601 tactcgccat agtagacgag tctacaccac gatctgtagt cacgtctaca cgggttggta
      661 ggtgttcagg agtgtctggt gggagatcag ccactgcctg ccggattcca agccttggac
      721 taggtatctg cggccgaggg cggcgtcggt gactttgatg atcctcaccc gtgattcgtg
      781 ctggggccgg ctgaggcgtt cccacacggc tccttctttc agggcgtcgt tgtgtacatg
      841 gttcaaccat cggcggggca tcggttgaga actacccgtt ctcatcggct gggttgtttg
      901 ggtacatgag gcgcatcggt agcagggatg actacagtgg aagcaccgaa accgctctga
      961 cctgcgcccc cggcaggatt cgaacctgcg accacaagat tagaagacat gtttacggta
     1021 tactctcacc atgaggttag cacaaacccg caggtagtca acgtgctaac gtaagacatc
     1081 ggacgctatc cgacgacaac aaaggctaag tatggagagc agtgagatgg acagcggctc
     1141 aagtcagatc acggaagtgg cgactgatgt cggaatcttc aagctgaaga tcaatgcaga
     1201 gggttgttgg cgcatcaccg atccagaaga caaactcgtt gccccgaata cgccgaccgg
     1261 gcgaaagcta atagcggcag ccgaagggag cgaacatgtc tgcgagctgc cttcacttga
     1321 aggcctttac cctggggatg tgtggacttg tccttgcggt gcgggctggg aagtcgatga
     1381 agtccgtacc gcagacgacg aatactttga ctggacgccc agattgagca gtggaggaac
     1441 gctatgagtg acgcgcttta catcattcga agtggtgaca gcacactcgg cgaagtgatg
     1501 acccacgaag aagtcgtgcg gtgggtcggt gtggctatca gctaccgctt agagaagaca
     1561 ttgtgtctga ctctacagag ccgtgccctg aacgccgccg ctgaggcagc agttcactgg
     1621 ctttctgaat cgctagcgaa agtgccgata aatcaggaga cggcacgcgc ctttgaatct
     1681 gtgcttaggc aacactccga ggtgtttcgg cagttggaga acaagtgatc gagtacaacg
     1741 ccctctacat ccagcgcgga tatctggata gattgctatt ttccgacact catttgcgcc
     1801 ctgacctttt cgagaagttg gatttaaccc tacacagtga acgaggaagt catgagcgat
     1861 tgggagacgc gcatcggtct agcggcggca aactgtgatt tcgctaggga ctgggatggc
     1921 ccttggcagc ataagaggga ccggttggat gagttgcact ctgagataga ccaattgctc
     1981 gatgagcgtt atccatgagc ggcggcttta cggacgcggt cgtgacgctt ttgatcttgg
     2041 ctgcattcgt agcggcgact ttcttcatct gcgcagtctg tgtgcgttgg cttattggct
     2101 acgacgcgcc cgctcggtta tggcgtcgtc ggcggatctg gcgcgatggt gattgccgcg
     2161 tcaacatcaa cttcgggact cccgagactg gacattggtg tgatcgctgc atgaagccga
     2221 gcgtatacgt tatgccgctg acggcactgc gccctgacgg agtaaaggac ttgggtgccc
     2281 aggctgttaa atgcctggat tgcggccacg gacgaatcga ggaaacacca tgagcatctg
     2341 tattcaccga gcttctatgc caacggacga gaacggtcgg ccgctctggc gtggagtaac
     2401 aacgcttacc tgtcgccttc cacgcaacca tgatggtgaa catcgccacg acgatgaggt
     2461 gtcatgggcc gattccgata tgacgcacgg catgggtccg attgcttacg cgcagggtgg
     2521 gttaccccaa cctggatccc ccggtgttct ctacgtagac gtcaagaacg tatagcgaga
     2581 gacgttgcgg actaagggtg atgggagtct ttttc
//
LOCUS       DATMTI010000975         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_800832, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000975 DATMTI010000000
VERSION     DATMTI010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800832"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..588)
                     /locus_tag="VN039_15415"
     CDS             complement(385..588)
                     /locus_tag="VN039_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47385.1"
                     /translation="MSSLFINEHNGQFTVEPAHSSTPYFTGRTQEEAIRWAQNNRPND
                     ALHVARVRHLPDKRNPDHWRRIH"
     gene            978..1313
                     /locus_tag="VN039_15420"
     CDS             978..1313
                     /locus_tag="VN039_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV47386.1"
                     /translation="MSENPFPERLRQVRDQRGFTQAQLATEAGLPSTTVSHFEAGTRK
                     PSFDNLRRLSKALNVSSDYLMGITDTREATGAASRIARHLGNATKEDIAFLEQMAETM
                     ANRSKPKDE"
     gene            1306..>1830
                     /locus_tag="VN039_15425"
     CDS             1306..>1830
                     /locus_tag="VN039_15425"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HWV47387.1"
                     /translation="MNDYRRQQIHAKALELLQSAGVTALSVDPIKIAEHLEIDVHAKP
                     ADVEGASGWLIKSGEHFGIAYATHIPSEGFQRFSIAHELGHYWLDSHPAHIFKNGSMH
                     QSHAGFGSADPIELEADYFASCLLMPATLCKRVIWGNKDGMAAVKALADKANTSLQAS
                     AIRYVELSRIPSVIV"
BASE COUNT          389 a          533 c          502 g          406 t
ORIGIN      
        1 cgatcaagtg aactccccct tccacgtggc ttgtcccggc attgtagtga acaggccacg
       61 acggggcacg cacgacggcc cggttcgcgg ttgttttcaa gccctgccga acggcccatg
      121 cccggtgtcg agagccctta atggtgtcag ttggctctaa ggctctgatg tgtagagatt
      181 tcccttgcgc tgtcactacg gaacacctgc gctactgaca gattcagggg tacaaaatcg
      241 tcgcattgac cggtttaagg gtgttttctg ctggtgcgct taccgccgtc aacgccagcg
      301 acacgccgga gcccgtgtgc cgttcctcca agaaaaagcc atcccgtgga gcggtatggc
      361 tagtgcaaca cagacgaatc agcttcagtg gattcgtcgc caatggtcgg ggttccgctt
      421 gtccggcaga tggcgtacgc gtgccacatg aagagcatcg ttgggtcgat tgttctgcgc
      481 ccaacgaatc gcctcctcct gagtgcgacc cgtgaaatac ggagtgctcg aatgggcagg
      541 ttcgaccgtg aactgcccgt tgtgctcgtt gataaacaaa ctgctcattt gtcacctcgc
      601 tgctcagttt gatgttggca actcaccgtc cgtccgtgtt ggcccgaacg tttaaccgcc
      661 aacggatgcg cccctgttcc tttgtgtgct gatgatggtt ctgcggtggg aaggggtcgt
      721 cctggttgca ggcgtcctcc ttcccgcacc cctggcaccg ataaattcct gacacaggta
      781 cctcttcacc cggctcgtaa gtcgtggtcc accacgcctt gtcggccacc gacttagtga
      841 gccttgaact atcgatgtac caggacatac actgcctccg aacgaactcg cctagaaggt
      901 gttgacggaa tcgacaatac gccgtgatgt cgattccgtc aatattttat tgacgaaaac
      961 ggagtacgtt cagcttcatg tcagaaaacc ctttccctga gcgacttagg caggtccgag
     1021 atcagcgggg cttcacccaa gctcagctgg ccaccgaggc gggcctccct tccacgaccg
     1081 tctcccactt cgaggcgggg acccgaaaac cgtccttcga caacctgaga cgactaagca
     1141 aagccctgaa tgtcagcagc gactacctca tgggtatcac cgacacccgg gaagccaccg
     1201 gggctgcatc tcgtatagcg agacatctgg gcaacgccac caaggaagac atcgcgttcc
     1261 ttgaacagat ggcggaaacc atggccaaca ggagtaagcc aaaggatgaa tgactatcgg
     1321 cgtcagcaga ttcatgccaa ggccctggag cttcttcaaa gtgcaggggt gaccgctctc
     1381 tctgttgacc cgataaaaat tgctgaacat ctggagattg atgttcatgc caagccagcg
     1441 gacgtcgaag gtgcgtcggg ttggctgatc aagtcaggcg agcacttcgg catcgcctat
     1501 gccacccaca ttcccagtga agggtttcag cgcttcagca ttgcccacga gctcgggcac
     1561 tattggctgg attcccaccc tgcgcatatc ttcaagaatg gctcgatgca tcagtcccac
     1621 gcggggttcg ggagtgctga ccccatcgag cttgaagcag actatttcgc atcatgcctc
     1681 ttgatgcccg ctacgctttg caagcgtgtg atatggggaa acaaagatgg gatggcggcg
     1741 gtcaaggctc tcgccgacaa ggccaatact tctttgcagg cgtctgccat ccggtatgtt
     1801 gagctgagca gaattccgtc agtcatcgtt
//
LOCUS       DATMTI010000976         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_843739, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000976 DATMTI010000000
VERSION     DATMTI010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843739"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="VN039_15430"
     CDS             <1..1427
                     /locus_tag="VN039_15430"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWV47388.1"
                     /translation="QNKPPASTRIERDTMGELAVPAEAYYGVQTARAIENFPISSLRM
                     PRAVIRAMGMIKRAAATANQSLGLLDKKPAEAVKQAATEVVDGRLDAEFPVDIFQTGS
                     GTSTNMNTNEVISNRATELLGGARGSKLVHPNDHVNLGQSSNDVIPTAIHIAASEMMQ
                     QQLLPALTRLHKALKGKAKEFDQIVKIGRTHLQDATPVRLGQEFGGYARQIELGIQRV
                     KRAQEALSEVALGGTAVGTGLNCHPKFSAKVMAIISKETGCSFKEAKNHFEAQSAQDS
                     LVEASGHLRTLAVSLMKIANDIRWLGSGPRCGLGEINLPETQPGSSIMPGKVNPVIAE
                     SVTMVCAQVIGNDVTVTVGGQAANFELIVMMPVMAYNLLQSIELLATVSDNFAAKCIE
                     GIKANEERCKSLIEESLAMCTALAPEIGYEAAAKLAKEAYKSGKTVRQIAKEQKVLSD
                     RRLSELLDPWRMTEPGGPVGSAGG"
     gene            1492..>2191
                     /locus_tag="VN039_15435"
     CDS             1492..>2191
                     /locus_tag="VN039_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HWV47389.1"
                     /translation="MMSSAGHSHSLIIIGLLVLLVSGTLFGCATTRRQSMQDEKSVIS
                     TMGTRGAHCCAMAKAVPRQAALAKAAVRFVGQSRIQVDGRNYTPDCSGFVRGVYASQR
                     VDLYGGLGELDGGNGVGRIFTHMVEHGRIHYGPTVHPGDLVFFHNTWDFNSDGLPNDP
                     LTHVGVVEKVDLDGTVVFVSSVSAGIKRYRMNLKHPDTHKTVDGRILNDFLRRKHVGD
                     AQGTYYLTGRLFAAF"
BASE COUNT          504 a          566 c          674 g          447 t
ORIGIN      
        1 atcagaacaa gccgccggca tcgactcgaa ttgagcgaga taccatgggt gaactggctg
       61 ttcccgccga agcgtactat ggagtacaga cagcacgggc gatcgagaat ttcccgatca
      121 gctcactccg tatgccgcgg gccgttatca gagccatggg catgatcaag cgggcagcgg
      181 ctaccgcgaa tcagtctctc gggttgttgg acaaaaagcc ggctgaagcc gtcaagcagg
      241 cagcgactga agtggtcgat ggcaggctgg atgccgagtt ccccgtcgat atttttcaga
      301 cgggatccgg tacgtcgacc aacatgaaca cgaacgaggt catttcaaac cgcgccaccg
      361 agctgctcgg cggtgcgcgc ggcagcaagc tggtgcatcc gaacgaccac gtgaatcttg
      421 gccagtcgag caacgatgtg attccgaccg ccattcacat cgccgcgtcg gagatgatgc
      481 agcagcagct gttgccggct ctgactcgat tgcacaaggc attgaaagga aaagcgaagg
      541 agttcgatca gatcgtgaaa attgggagaa cgcatctaca agatgctacg ccggttcggc
      601 ttggccaaga attcggcggc tatgccagac aaatcgaact cggcatccaa cgggtcaagc
      661 gggctcaaga ggctttgagc gaagtcgcat tgggtggaac ggccgtcggg acaggcctga
      721 actgtcatcc gaaattttcc gccaaagtca tggcgatcat atccaaggaa accggctgct
      781 cgttcaagga agccaagaat cattttgaag cgcagtcggc acaggactca ctggttgaag
      841 ccagtggcca tctacggacc cttgcagtca gtcttatgaa gatcgccaac gatattcgct
      901 ggcttgggtc cgggccacgc tgtggactcg gcgagattaa tttgcccgag acgcagcctg
      961 gttcgtcgat catgccgggg aaggtcaatc ccgtgattgc cgaatccgtc acgatggtct
     1021 gcgcacaggt gatcggtaac gacgtgacgg taacggtagg cggtcaggcg gcaaattttg
     1081 agctcatcgt gatgatgccg gtcatggcat acaacctgct ccagtcgatc gaattgctgg
     1141 ccactgtttc tgacaacttt gctgccaagt gtatcgaggg gatcaaggcg aacgaagaac
     1201 gttgtaagag cctcatcgaa gaaagcctgg cgatgtgtac cgctcttgct ccggaaatcg
     1261 gctacgaagc agcggcaaaa ctggccaaag aagcctataa atccggaaaa acagtgaggc
     1321 agatcgccaa agaacaaaag gtattgtccg acagacgcct gtctgaactc ctcgatccgt
     1381 ggcgtatgac cgagccgggc ggcccagtgg ggagtgcggg aggatagctg agggggagag
     1441 ctgaacggtg cgcaggtatt gtcttacggc agtgggtctg tatactaatg catgatgagt
     1501 tcagccggcc acagtcactc gctcatcatc atcgggcttc tggtgttgct ggtttccggc
     1561 acgctcttcg ggtgtgccac gactagacga cagtctatgc aagacgagaa gagcgtgatt
     1621 tccactatgg ggacgcgcgg agcacattgt tgcgccatgg ccaaggcggt tccacgacag
     1681 gcggcgctgg cgaaggcggc ggtgcggttt gtcggacagt cgcgcattca ggtggacggg
     1741 cggaactaca ctccggattg ctccggcttt gttcgagggg tgtatgcctc gcagcgtgtt
     1801 gacttgtatg gtgggttggg ggagctggat ggcggcaacg gcgtcgggcg catctttacc
     1861 catatggtgg aacacggacg gattcattac ggccccaccg tccatccggg agacctggtc
     1921 ttctttcaca atacctggga tttcaacagc gacggattgc ccaacgaccc gctcacacat
     1981 gtcggcgtcg tcgagaaggt ggaccttgac ggaaccgtcg tgttcgttag ttcggtttca
     2041 gcggggatta aacggtacag gatgaatctc aagcatcccg acacacacaa gaccgtggat
     2101 ggacgaatcc tgaacgattt cttgcgacgc aagcatgtgg gagacgctca agggacctat
     2161 tatcttaccg ggcgattgtt cgccgctttt g
//
LOCUS       DATMTI010000977         4404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_587494, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000977 DATMTI010000000
VERSION     DATMTI010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587494"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VN039_15440"
     CDS             <1..282
                     /locus_tag="VN039_15440"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV47390.1"
                     /translation="FQACPYLGGVQQESCGFCSEGLTGGKKKKGLTLTSLAAARESFE
                     KDYIVSVLERADGSRTTASRLLGLSRKALWEKCKRYGIPSAHSDAEDES"
     gene            384..791
                     /locus_tag="VN039_15445"
     CDS             384..791
                     /locus_tag="VN039_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47391.1"
                     /translation="MNIWRHVIAISWGVVLLWGVVYAAEPAEVAKFVNARIEIGEMMT
                     NYFKGGQSYGEGQRPSQEQMRQMGADINSKLTALLAKHDLTLDDYRKRSPDVFADDAA
                     VKRYLNEHPDLKQRYEALPLDRMGRGGSTGRGY"
     gene            808..1779
                     /locus_tag="VN039_15450"
     CDS             808..1779
                     /locus_tag="VN039_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWV47392.1"
                     /translation="MEYKHLGRSGVKVSRLCLGTMNFGPQTSEQNSFALMDRALELGI
                     NFFDTANVYGWKLGEGWTEQIIGRWFAQGGGRRDKVVLATKVYGRMGEWPNQSRLSAM
                     HIKRACEDSLRRLKTDWIDVYQMHHVDRETPWEEIWQAMEQLVREGKVVYVGSSNFAG
                     WHLAQAQEAARSRHFLGLVSEQSLYNLNERTIELEVIPACEAYGIGLIPWSPLGRGLL
                     AGVLPSANSGRRADEDLKKEVAQSRSKLEAYEGLCAGIGERPANVALAWLLHQRAVTS
                     PIIGPRTMEQLEGTMRALSLSLGSDILKQLDELFPGPGGAAPESYAW"
     gene            complement(1889..2305)
                     /locus_tag="VN039_15455"
     CDS             complement(1889..2305)
                     /locus_tag="VN039_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47393.1"
                     /translation="MQTLDIHRPEMPDLQFVLLVTALCTSRLTALNISEALRATIFNR
                     CWTLIHDSPPPRRLEDRVLDLRPWTELTVEAMVETIRVVLTEAGIQTLAWDHAPSEPT
                     RTSTPEAKPLIERFAQLYPQPPEETDRGHAANSVPH"
     gene            complement(2397..3287)
                     /locus_tag="VN039_15460"
     CDS             complement(2397..3287)
                     /locus_tag="VN039_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWV47394.1"
                     /translation="MSLDYVRFTPGFDSFMPKEYRDMVEHGPFGKKTTVSQMGNFKEI
                     LEEHPMCAGCAMTLFIRLAIVAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRIRFGDKPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTTRGAVLKMAPLGKKFEKMDMVGMAKIAGCSYIATVVPNNPRRVESCI
                     KKAVLIAREVGPTYIQAYTSCNIEYAIPTDKVMEDAKAVEDDRYKFTEYVSEDAKAYL
                     ADRYGYKEFIQKPGATITKV"
     gene            complement(3480..>4404)
                     /locus_tag="VN039_15465"
     CDS             complement(3480..>4404)
                     /locus_tag="VN039_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWV47395.1"
                     /translation="AFGGARVFTTTAGPGTMRAMENFPMWAGARLPIQCIVTCRGINS
                     PLPIQPDTIEIAYLMNTGMLVWHAETAQDFYDWILKGYMVSEEPDVHLPLALCCDGFF
                     VTHTKDVVGLTPAEMCLPPYDPYRSPVPCMDMECPPVRMMRDPFVMKSNYISYATHAS
                     WQQEVWAAAERSRKHTIAWLDGLIDIEDGDADILIISSGTAVSQGREAMRLLADEGVR
                     VGLVKIKTLRPWPEEELREATKHASHIIVPEFNVIGWMAKEIKATIPNSERVVAGPRV
                     CGGMTLPPEVIVEEVKKAIGMRSGVLAGRGG"
BASE COUNT          894 a         1208 c         1325 g          977 t
ORIGIN      
        1 tttcaagcct gtccctacct gggaggcgtc caacaagaat cctgcggttt ctgtagtgaa
       61 ggtttgaccg gaggaaaaaa gaagaaaggt ctgactctga cctcgctggc cgccgcgcga
      121 gaaagttttg agaaggacta catcgtctcc gtattggaac gtgccgatgg gagccgcacg
      181 accgcctcca gactcttggg attgtccaga aaggcgcttt gggaaaaatg caaacgctat
      241 ggtattccgt cggcacacag cgacgctgag gatgaaagct aaaggccgct cattcccata
      301 ccgactggat tccaaattgt cgcgcggtcg taaaggagtg tcgtcgcgtt aaaggatgtt
      361 atgtatttgc tgtaggaggg atcatgaata tttggcgcca tgtcatcgct atttcgtggg
      421 gagtcgttct actatgggga gtcgtgtatg ccgccgagcc ggccgaagtg gcaaagttcg
      481 tcaacgcccg aatcgagatc ggtgagatga tgacgaacta tttcaaaggg ggtcagagtt
      541 atggggaggg acagcgtccg tcccaggagc aaatgcgaca aatgggagca gacatcaata
      601 gcaagctcac ggcgcttctc gccaagcacg atttgacgct cgatgactat cgcaagcgca
      661 gtccggatgt ttttgcggat gatgcggcgg tcaagcgcta tctcaacgag catccggacc
      721 tcaaacaacg ttacgaggcg cttccactcg atcgaatggg gcggggcggg agtacagggc
      781 gagggtatta ggttcgcagg cgcactcatg gaatacaaac acctcggtcg ttctggagtg
      841 aaagtcagtc ggctctgtct gggtacgatg aatttcggcc cgcagacgag cgagcagaac
      901 agcttcgctc taatggatcg ggcattggag ctcggtatca atttttttga tacggcaaac
      961 gtgtacggct ggaagctcgg tgaaggctgg acagagcaga tcatcggccg ctggttcgca
     1021 caaggaggag gccgtcgaga taaggtggtg ctggccacga aagtgtacgg tcgcatgggc
     1081 gaatggccga atcaatcgcg cctctcggcc atgcacatca agcgagcgtg cgaggatagt
     1141 ctccggcggt tgaaaacaga ctggatcgat gtgtaccaaa tgcatcatgt cgatcgggaa
     1201 acgccgtggg aagaaatctg gcaagcaatg gagcagttgg tcagggaagg caaagtagtc
     1261 tatgtgggca gcagcaattt cgccggttgg cacttggccc aggctcagga agccgcgcgt
     1321 agccgtcact ttttggggct ggtgtcggaa caaagtctgt ataacctcaa tgagcgtacg
     1381 attgaactgg aagtcattcc tgcctgtgag gcctacggca tcggtctgat cccgtggagt
     1441 ccgctgggaa ggggcctctt ggccggggtt ctgccatccg caaacagcgg gcgtcgcgcg
     1501 gatgaggatc tgaagaaaga ggtggctcag tctcgttcca aattggaagc gtacgaaggc
     1561 ctgtgtgcag ggattggtga aagaccggcg aatgtcgcgc tcgcctggtt gctgcaccaa
     1621 cgagcagtca cttcaccgat tatcggcccc cgcacgatgg aacagctgga aggcactatg
     1681 agggcgttga gtctttccct gggcagcgat atcttgaagc agctagacga gcttttccct
     1741 ggtcctggag gagccgcacc agaatcgtat gcctggtaat ccgttcatcg tccgttctgc
     1801 ttgtttttcc tcttcgtcga tggccgactg gcttgaccgc caaaactaag gacggatcac
     1861 agcccgtcag ctcattctct ccgtctcatc aatgcgggac agaatttgcc gcatggcctc
     1921 tgtcagtttc ctcgggtggt tgaggataca gctgcgcgaa tcgttcgatc agtggcttgg
     1981 cttcgggcgt gctggtgcgt gtcggctcgc tgggcgcatg gtcccaggcc aaggtttgaa
     2041 tcccagcctc cgtgagcacc acgcgtatag tctctaccat cgcctcgacg gtgagttcgg
     2101 tccagggtcg gaggtccagg acccgatcct ccagtctgcg gggcggaggg ctgtcatgaa
     2161 tgagcgtcca gcaccgattg aaaatcgtgg cgcggagggc ctctgagata ttgagcgcag
     2221 tcagacgaga cgtacagagc gccgtcacta acaggacgaa ctgaaggtcc ggcatctcgg
     2281 ggcgatggat atcgagtgtt tgcatgatca atggcctccc acctgagacg ccggaaaggc
     2341 cttcagcgat gacgacgcct cggttacgga tggttgagac ttacgcgtcg gcgtgattac
     2401 accttcgtga tcgtcgcccc tggcttctgg atgaactctt tgtagccgta tcgatcggcc
     2461 aaataggcct tggcgtcttc gctgacgtac tcggtaaatt tgtatcgatc gtcctcgacg
     2521 gccttggcat cttccatgac cttgtcggtc gggatcgcgt actcgatatt gcaggacgta
     2581 taggcctgga tataggtcgg accgacctcg cgagcgatca gcacggcctt cttaatgcag
     2641 ctttccaccc gccgcggatt attcggcacg acggtggcaa tataggagca gccggcgatc
     2701 ttggccatgc cgaccatgtc cattttctcg aacttcttgc cgagcggggc catcttgagc
     2761 accgcgcctc tggtcgtcat gccgctttct tgtccgccgg tattcccgta gacttcgttg
     2821 tccagcatga tcgtcgtgaa ccgctctttc cggaaccagg aatggaggac ttgctggaat
     2881 ccgatgtcgg cggtgccgcc gtcaccggcc atcacgacga catccttagg cttgtcgccg
     2941 aaacgaatcc gcaatccgcg cgacaatcca ctggcgacgc cgttctggtc cccatagttg
     3001 ccgtacacga atggaatcgc cgcctgtgaa atcgccagcc ggccgcagcc tgccgtcccg
     3061 actgtaatcg tgtcttcggg attcgggaac gcgacgatgg cgagccggat gaacagggtc
     3121 atcgcacagc cggcgcacat gggatgctcc tcaaggattt ctttgaagtt gcccatctgc
     3181 gacacggtcg tcttcttccc aaagggcccg tgttccacca tgtcccgata ttctttcggc
     3241 atgaagctat cgaatcctgg ggtgaacctc acataatcga gactcatctg gcacctcgct
     3301 ttcgctgttg tccgcagttg cggtggtggt gtgtatatcg cttgcagagt caatggcgtt
     3361 gccgtggcct tcttctcctt cgccttggcg gcgacttcgg cgagcaatgc cttcgccggt
     3421 tactgacata cttcctaaat gcttgtctgg ttgttccctc ctggtgcgac tgagtacact
     3481 catccaccgc gtcccgccaa cactccggat cgcatcccga tcgccttttt gacttcctcc
     3541 acgatcactt ccggcggcaa ggtcatgccg ccgcagacgc gcggtccggc gacgacgcgc
     3601 tcgctgttgg ggatcgtggc cttgatttct ttggccatcc atccgatgac attgaactca
     3661 ggcacgatga tgtgcgaggc atgcttggtc gcttcgcgaa gttcttcttc cggccatggg
     3721 cgcagggtct tgatcttgac gagtccaacg cgcaccccct catcggccag cagacgcatg
     3781 gcttcccgcc cctgggaaac ggcggttccc gaggagataa tgaggatgtc ggcatcgccg
     3841 tcttcgatat cgatcaaccc gtccagccag gcgatcgtgt gcttgcggga ccgttccgcg
     3901 gcggcccaga cttcctgttg ccaactcgcg tgcgtggcgt agctgatgta gttgctcttc
     3961 attacgaagg gatcgcgcat catccggacc ggtggacact ccatatccat gcagggaacc
     4021 ggcgagcgat agggatcata gggtggcaaa cacatttcgg cgggagtcag gccgacgacg
     4081 tccttcgtat gggtcacgaa gaagccgtcg caacagaggg ccaacggcaa atgcacgtca
     4141 ggctcttccg acaccatgta gcccttgaga atccagtcgt agaaatcctg tgcggtttct
     4201 gcatgccaga cgagcatgcc cgtattcata aggtaggcga tttcgatcgt gtccggctgg
     4261 atcggcaggg gggaattgat gccacggcag gtcacgatac attggatcgg caatctggct
     4321 ccggcccaca tcggaaagtt ttccatggcc cgcatggtgc ccggtccggc ggttgtcgtg
     4381 aagacacggg ccccgccgaa ggcg
//
LOCUS       DATMTI010000978         1596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_290403, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000978 DATMTI010000000
VERSION     DATMTI010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290403"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..338
                     /locus_tag="VN039_15470"
     CDS             42..338
                     /locus_tag="VN039_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWV47396.1"
                     /translation="MTQSVDEPSVRARILVAGHVQGVGFRAFAARSATRLDLVGGVRN
                     LDDGRVVLEVEGRRTVIKALVRELKTGPPAARVTKIETEWSVAIGRYSDFSIWY"
     gene            352..1305
                     /locus_tag="VN039_15475"
     CDS             352..1305
                     /locus_tag="VN039_15475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV47397.1"
                     /translation="MSRPHDEESELSEARRQTVDDMDASEPASAPDPGERETGRSEGL
                     DTLKSYLREVRRSTLLTFKQEQQLGKRVMAGDEHARQQMIESNLRLVISIGKRYMHRG
                     FPFSDIVEEGNLGLIKAVEKFNYKRGFRFSTYASWWIRQYIERAIINQGKLVRLPVHV
                     VERLNRYLNRVEQLVQELGREPRAAEVAAKMKTSEEEVLDLKQLVRTTCSLDSPLNDR
                     TDTFLRDVIEDPVGLSPDETADGVRRRMELMAWVRELPEKEQTVIVSRFGLDGDEAKT
                     LEEIGRSMGLTRERVRQIEMAALVRLRNTIERKTMTQADLL"
     gene            1310..>1596
                     /locus_tag="VN039_15480"
     CDS             1310..>1596
                     /locus_tag="VN039_15480"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine phosphoribosyltransferase family protein"
                     /protein_id="HWV47398.1"
                     /translation="MTAVNYQALIREVPDFPKPGILFYDITTLLKNSAAVRSLADQLT
                     TRYQDRGITKVVGIESRGFIFGGILASHLGAGFVPVRKPGKLPADCYEVKY"
BASE COUNT          364 a          378 c          504 g          350 t
ORIGIN      
        1 cataagtggt cgtttacact taaagatttt gataggaccg gatgactcaa tcggttgatg
       61 aaccgtcggt tcgagcgcgc atcctcgtgg ctggtcatgt gcagggggta ggatttcgcg
      121 cgtttgcggc acgatccgcc actcggcttg atctcgtcgg tggcgtccgc aatctcgatg
      181 atggtcgggt tgtgctggag gtggagggca gaagaacggt gatcaaagcg cttgtacgtg
      241 aattgaagac cgggcctccg gccgcccgtg tcacaaagat tgagacggaa tggagtgttg
      301 cgatcgggcg atattcagac ttcagcattt ggtattagaa aacgaggaat catgagtcga
      361 ccgcatgacg aggagtccga gttgagcgaa gctcgaaggc aaaccgtcga cgatatggat
      421 gcctcagaac cggcgagtgc gccggatccg ggtgaacggg aaacgggtcg ttctgagggt
      481 cttgataccc tcaaaagcta cttgcgggag gtccgccgat cgaccctgct gaccttcaaa
      541 caagaacaac agctcggtaa acgggtgatg gccggcgatg agcacgctcg gcaacagatg
      601 atcgaatcga atctgcgact tgtcattagc atcgggaagc gttacatgca ccgggggttt
      661 ccgttttccg atatcgtgga agaaggcaat ttaggcttga tcaaagcggt cgaaaaattc
      721 aattacaagc gagggttccg gttcagcacc tatgcgtctt ggtggatccg gcagtacatc
      781 gagcgggcga ttattaatca aggcaagctg gttcgtttgc cggtgcatgt ggtagagcgc
      841 ctcaatcgtt atctgaatcg cgtcgagcag ctggtacagg aacttgggcg ggagccgagg
      901 gcggccgaag tggcggccaa gatgaagaca tcggaagagg aagtcttaga tctgaagcaa
      961 ttggtgcgca caacctgttc gctcgatagc ccgctcaacg accgcactga taccttctta
     1021 cgcgatgtga tcgaggatcc ggtcgggctg tcgcctgatg agaccgccga cggagttcgg
     1081 cggaggatgg aactgatggc ctgggtgagg gagttgcctg agaaagagca aactgttatt
     1141 gtgtcacggt tcgggctcga cggagacgaa gccaagacgc tcgaggaaat cggccgtagc
     1201 atggggctga ctcgtgagcg cgtccggcag atcgagatgg ctgcgttggt tcggcttcgc
     1261 aacaccatcg agcgaaaaac tatgacacag gcagacttgc tctaatcccg tgaccgccgt
     1321 caactatcaa gcactcattc gagaagtgcc ggacttcccg aagcccggca ttctgtttta
     1381 tgatatcacc acgctcctga agaattctgc cgccgttcgg agtcttgccg accagttgac
     1441 gactcggtat caggatagag ggatcaccaa ggttgtaggg attgagtccc ggggatttat
     1501 tttcggcggg attctcgcgt cgcatctcgg ggccgggttt gtcccggttc gcaagcctgg
     1561 aaaacttccg gctgattgtt atgaagtgaa gtatag
//
LOCUS       DATMTI010000979         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_672745, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000979 DATMTI010000000
VERSION     DATMTI010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672745"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..862)
                     /locus_tag="VN039_15485"
     CDS             complement(14..862)
                     /locus_tag="VN039_15485"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWV47399.1"
                     /translation="MSRLDLHLHTTHSDGSCTPTEVVNMAHRAGVTALAITDHDILTG
                     VAEAIAAGQEYGIEVIPGVEISSLAGNSELHILGYFLDCQNPDLLERLKTLRDGRHRR
                     NPRIIERLHTLGIDITYDEVRALAGTGSIGRPHIARVLMDKHVVASAKEAFDRFLAEG
                     KPAYVPRELPNPAEAIRWIKAARGLAVLAHPTWVKVTEQPLVDLVRQLKADGLDGVEV
                     YYSTHAARQTREYLSLAQQLGLLVTGGSDFHGLTKPDIEVGIGKGALHIPASLLPKMK
                     EAAGRL"
     gene            complement(859..1803)
                     /gene="folB"
                     /locus_tag="VN039_15490"
     CDS             complement(859..1803)
                     /gene="folB"
                     /locus_tag="VN039_15490"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWV47400.1"
                     /translation="MDAHVVIEQLEFRGRCGVMPEERARPQPLAVDLDLELDGRLEAA
                     GLSDDLVHTIDYAKVAQHVIDIGTLQESQLLETLAERLLSALFDKFPIGRITLWLRKL
                     HPPISFITRSVGIRIERTRLAQQVIRADPPPAEFLVRQLHRLPKGKVLDVASGSGRHA
                     LFLASLGYQIEAVDRDEQALAQLSAVARARHLTGINLQVLDLEQPSHEPNLGHEAYDV
                     ILVFFYLNRPLFPHLIDALKPGGVLLYETFTVDNHVQHRHPKRREFCLASCELLSLTL
                     GLRILHYDEGLHEGTSRSEPVYTAQLMAQKPLSPGPSI"
     gene            complement(1888..>2752)
                     /locus_tag="VN039_15495"
     CDS             complement(1888..>2752)
                     /locus_tag="VN039_15495"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWV47401.1"
                     /translation="LNFKQAKRAIQNIVDLMMQDEPIMLGLTTLRCHDQYTHNHSVNV
                     SLLSIALANRSGYPKVELADLGLAALFHDMGKSTIPLEVLNKPGEFTEDEWTMMRNHP
                     TEGVLSLTQLRGITNLPARMAAASFEHHMNLDYSGYPKLEIPWKLSLTGRILTIADCY
                     DAMTSSRVYRREPMSPSKVLNMMLAKSGKSFDATLLKLFVNCVGIIPIGSLVVLDSDE
                     FAVVLKPAADKAESERPVVKVIADAQGTPLDNGPELDLTEKDGVGEYRHSIIRLIDNT
                     EHKFDTSRYFL"
BASE COUNT          578 a          706 c          859 g          609 t
ORIGIN      
        1 ggaagatact gcgctagagc cgtccggcgg cttccttcat cttgggaagc agtgaggctg
       61 gaatgtgcag cgcgcccttg ccgatgccga cttcgatgtc gggtttcgtg aggccatgaa
      121 agtcgctgcc accggtgacc aataacccga gttgttgggc cagactgagg tattctcgag
      181 tttgtcgcgc agcatgcgta ctatagtaga cctctacacc gtcgagcccg tcggctttga
      241 gctgtcgaac gagatcaacc agcggctgct ctgtcacctt cacccacgtc gggtgggcca
      301 ggacagcaag cccgcgagcg gctttgatcc agcggatcgc ttcggcaggg ttcgggagct
      361 cccggggaac ataggccggt ttaccctcgg caagaaaacg atcaaaggct tctttggcag
      421 aagcgacgac gtgtttgtcc attaagacgc gggcgatgtg cggtctgccg atcgagccgg
      481 tgccggcgag tgcgcggact tcgtcgtacg taatgtcgat tcccaaggtg tgtaatcgtt
      541 cgatgatccg tggattccgg cgatgacgac cgtctcgcag ggtcttcaga cgctcgagca
      601 aatcggggtt ctggcaatca agaaagtacc caagaatgtg taactccgag ttgccggcaa
      661 gcgaactgat ttccactccg ggtatgacct cgattccata ctcttgtccg gccgcaatgg
      721 cttcggcgac accggtcagg atgtcgtgat cggtgatcgc caaggccgtc acccctgcgc
      781 ggtgtgccat gtttaccacc tcagtgggtg tgcagcttcc gtcggagtga gtggtatgca
      841 gatgaagatc caggcggctc atatcgacgg tcctggagac aagggtttct gtgccatgag
      901 ttgggccgtg tagacgggct ccgatctgct cgttccttcg tggagccctt cgtcataatg
      961 aagaatgcgg aggccgagag tgaggctcaa gagttcacac gatgcgaggc aaaattctcg
     1021 acgctttgga tgtcggtgtt ggacgtggtt gtcgacggta aaggtttcat acagcaagac
     1081 accgcctggt ttcaacgcgt cgatgagatg agggaagagc ggtcgattca ggtaaaagaa
     1141 gacgagaatg acatcgtacg cttcatgtcc aagattcggt tcatgtgacg gctgctcaag
     1201 atcaagcact tgcaagttga tgccggtgag gtgtcgtgct cgggcgacgg cagaaagttg
     1261 tgcgagggcc tgctcatcgc gatcgacggc ttcgatctga tagcccagcg aggcgaggaa
     1321 cagtgcgtga cgcccgctgc cggaggccac atcgagaacc ttccctttgg gaaggcgatg
     1381 aagctgtcgg acgagaaact cggccggtgg cggatctgcg cggatgactt gctgcgcgag
     1441 ccttgtgcgc tcaattcgga tcccgacgga gcgggttatg aaggaaatcg ggggatgcag
     1501 ctttcgcagc cacagcgtga tacgaccgat ggggaattta tcgaacaggg ctgacaggag
     1561 tcgttccgcc agcgtttcca gcagctgaga ttcctgaagg gttccaatgt cgatgacatg
     1621 ctgagccact ttcgcgtaat cgatcgtgtg aacaagatcg tcggacaatc ctgctgcctc
     1681 aagacgaccg tccaattcca aatccagatc gacggcaagc ggttgcgggc gagcgcgttc
     1741 ctcgggcatc acgccgcagc ggccgcggaa ttccaattgc tcgatgacga catgtgcatc
     1801 catgtacaca ttgtcaaaga tcggtttcag gctgtcaagc gaggaagaga gatgggtttg
     1861 gaaggcgaca ggtgccgttt acgacggcta aaggaagtaa cggctcgtat cgaatttgtg
     1921 ctcggtattg tcgatcaggc gtatgatgct gtgacggtac tcgccgacgc cgtctttctc
     1981 ggtcaggtcc aactcgggac cgttatcgag gggagtcccc tgcgcatcgg cgatgacttt
     2041 gacgaccggt cgttcgctct cggccttgtc ggcggcaggc ttgagcacga cggcgaattc
     2101 atcggagtcg aggaccacca ggcttccgat cggaatgatt cccacgcaat tgacgaagag
     2161 tttgagcagg gtcgcgtcga aacttttccc ggatttcgca agcatcatat tcagcacttt
     2221 cgagggcgac atcggctccc ggcggtagac gcgggaagac gtcatggcat cgtaacaatc
     2281 ggcgatcgtg agaatgcgcc ccgtcagcga aagtttccac ggtatttcta atttggggta
     2341 gcccgaatag tccaagttca tgtgatgttc aaaggatgct gcagccatgc gcgccggaag
     2401 gttggtgatg ccgcgtaact gcgtgagact caagactcct tcggtggggt ggttgcgcat
     2461 catcgtccat tcatcttcag taaactcccc cggtttattc agcacttcaa ggggaatagt
     2521 cgactttccc atgtcgtgaa agagcgcggc caagccgaga tcggccagct cgactttcgg
     2581 atagccggat cggtttgcaa gagcgatgga gagcagtgag acgttgacgg agtggttgtg
     2641 cgtgtactga tcgtgacagc gaagcgttgt gaggcccagc atgatcggct cgtcctgcat
     2701 catcaggtcg acgatattct gaatggcccg tttcgcctgt ttgaagttca gc
//
LOCUS       DATMTI010000980         3117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_800940, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000980 DATMTI010000000
VERSION     DATMTI010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800940"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VN039_15500"
     CDS             complement(<1..1069)
                     /locus_tag="VN039_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV47402.1"
                     /translation="MNEPSHITQADADRLEGQIHLYQPASPILELRFVSCAYDPNRLA
                     IRDISFSAREGEILCLLGPSGCGKTTILRAIAGFEPIRSGQIFLSDRLVSSSSETIPT
                     EERRVGMVFQEYALFPHLRVADNIAFGLRHLSRAERRCRVQEMLRLTGLEGLDRRYPH
                     ELSGGQQQRVALSRALVQHPVLLLLDEPFSNLDPDMAGRMRQEVHTLLRRMKTTTILV
                     THDHDEAFAMADRIAVLNQGVLEQMDAPEVIYHLPATRFVADFVGQADFLTGQIRQGM
                     VHTELGEFPDTLNSAEGSTVVVMIRPDDIHLTPSKSAESRIMARQFRGSENLYTICLP
                     SGQVVHSSERSTSVYHEGTAVE"
     gene            complement(1077..2660)
                     /locus_tag="VN039_15505"
     CDS             complement(1077..2660)
                     /locus_tag="VN039_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWV47403.1"
                     /translation="MIGIHRSSVSPLQLAALASATLVLLPLGYVTALALSADPSIWHR
                     LWTTRVPELLLNTVSLAGTVALLTLVLGVSTAWMVTRFEFPGRRLWEVGLVLPLAMPT
                     YVLAYVYNHLLGFGGPVEHLWQMIIGPQARIISPQSFWGVTVVMALDTFPFVYLLTRS
                     ALLSFNVSFEEVARTCGASPLRRTLFVTLPLLRPSIVAGLALVILYVVSDFGAVSLLR
                     YQTLTYAVFQQMTGRSDNQAASILSILLVILALLFLLTERWFRRKSRFYQTSGRFRVP
                     QRIRCGWLHTVALTACLATVIGLAFGIPAYLLVTWSLSPEVQAILDARFFGFVWNSAL
                     LSTLAATAGVIVGLPLAYLASRKPTWFNTGCLQAAYAGYVLPGPVAALAVLVLFLKVL
                     PFLYGTVIVLIVAYVLHFLPAGLQSLEPSIQQITPNLEEVSRTLGLTVRDTWRRVTLP
                     LIRNGVIVAWVLIFLQTMKELPATLLLRPVGFDTLAIRVWLEASEEYYQLAAPSALLI
                     VLVGLPALALLLSRDWRAV"
     gene            complement(2676..>3117)
                     /locus_tag="VN039_15510"
     CDS             complement(2676..>3117)
                     /locus_tag="VN039_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWV47404.1"
                     /translation="DNAGTQVYQKSSQIVDAVAKGQVAMGIVNHYYVYRHLATQPAAP
                     LAVLMPDQQEGGMGAIMNVAGIGVLKHSPRVENAKLLVEFLVAQAGQKMFADLDKEYP
                     LHPEVKADPALIERKSFRAALVPLAKLAELREPTLLLIEQVGMR"
BASE COUNT          740 a          837 c          960 g          580 t
ORIGIN      
        1 gctcgacggc cgtcccttca tggtagacac tggttgaacg ttcactgctg tggacgactt
       61 gcccggaggg aaggcagatt gtgtacagat tctccgagcc tcggaactgg cgggccataa
      121 tccgggactc agccgacttg ctgggcgtca gatggatatc gtcggggcgg atcatgacga
      181 caaccgtgct cccctcggca ctgttcagcg tatcggggaa ctcacccagt tcggtatgca
      241 ccatgccttg tcgaatctgt ccggtgagaa agtcggcttg gcctacaaaa tcggccacga
      301 acctagtggc tggaaggtga taaatcactt ccggggcatc catctgctcg agcactccct
      361 gattcaggac tgcaatacgg tccgccatcg cgaaggcttc gtcatgatca tgcgtcacta
      421 aaatagtcgt ggttttcatc cgccgtaaga gggtatgaac ttcttgacgc atacggccgg
      481 ccatatcagg gtccaggttg ctgaacggct catccaggag gagcaagacg ggatgctgca
      541 cgagtgcgcg tgaaagggcc actcgttgct gttgccctcc cgacaactca tgcgggtaac
      601 gtcggtcgag tccttcgagg ccggtcagcc gcagcatttc ttggacacga catctccgtt
      661 ccgcccgcga caggtggcgg agcccgaagg cgatgttatc ggcgacgcgc aggtgaggga
      721 aaagcgcata ttcctgaaag accataccca cacggcgttc ctccgtcgga atcgtttccg
      781 aggaggagga gaccaatcgg tccgataaaa atatttgccc ggatcgaatc ggttcaaaac
      841 cggcgatggc tcgcagaata gtggttttgc cgcaaccgga cggtcccagc aaacagagaa
      901 tttcgccttc acgcgccgaa aatgaaatat cgcggatggc gagtcggttg ggatcatagg
      961 cacaagacac gaaacggagt tccaagatag gagaggccgg ctggtagagg tggatttgac
     1021 cttcgagtcg gtcggcatcc gcctgcgtga tgtgcgacgg ttcgttcatg agggttttac
     1081 actgcccgcc aatcacgaga cagcaacaag gccagcgccg gtaagccgac gagcacgatc
     1141 aataacgcgg acggagccgc cagttggtaa tactcttcgc tggcttccag ccagacacga
     1201 attgccaagg tgtcgaatcc caccggtcgc agcagtaacg tcgcaggaag ctccttcatc
     1261 gtctgcaaaa agatcaagac ccatgccacg atgaccccgt tgcggatgag cggcagagtc
     1321 acgcgacgcc aggtatcccg aacggtaaga cccagggtgc gggagacttc ttcaagatta
     1381 ggtgtgattt gttgaatcga cggctcgagt gactggaggc cggccgggag aaaatgtaag
     1441 acataggcga cgatcaatac gatcaccgta ccatacaaga acggcagaac ttttagaaag
     1501 aggaccaata ccgcgagtgc agcgaccggt cccggcaaca catagccggc ataggcggct
     1561 tgcaaacagc cggtgttgaa ccaggtcggt tttcgactgg cgaggtaggc gaggggcagt
     1621 ccgacgatca caccggccgt ggcggccagg gtcgacagca aggcactgtt ccagacgaag
     1681 ccgaagaagc gggcgtcgag aatcgcctgg acctccgggg acaggctcca tgttaccagt
     1741 aaataggcag ggattccgaa ggccaagccg atcacggtgg ccaagcatgc cgtcagagcg
     1801 acagtgtgca accatccgca ccggattcgt tgcggcaccc gaaagcgtcc ggaggtctga
     1861 taaaaccggc tttttcgacg aaaccatcgt tcggttaata agaacaggag cgccaggatg
     1921 accaacaaga tgctcaggat gctcgcggcc tggttgtcgg accgtccggt catttgctga
     1981 aacacggcat aggtgagtgt ttggtagcgc agtagagaga cggcaccgaa gtctgagacg
     2041 acatacagga tgacaagagc gagtccggcg acgatggacg gacgcagcaa cggcagggtg
     2101 acgaacaatg ttctacgaag cggtgaagcg ccacatgtac gggccacttc ttcaaaggac
     2161 acgttaaaac tcaggagcgc gctgcgtgtg agcaagtaaa cgaagggaaa cgtgtcgagc
     2221 gccatcacga cggtgacgcc ccagaaactt tggggtgaga tgattctggc ttgagggcct
     2281 atgatcatct gccagagatg ttctactgga ccgccgaatc ccaagagatg gttatacaca
     2341 taggccaaga cgtacgtcgg catggccagc ggcagtacca gaccaacttc ccacaggcgg
     2401 cggccaggga attcgaatcg cgtcaccatc catgcggttg atacgccgag cacgagtgtg
     2461 agcaaggcca cggtaccggc caaggaaaca gtgttcaaca gtagttcagg gacacgggtg
     2521 gtccagagtc gatgccaaat cgaaggatcg gccgacaagg cgagggccgt gacgtagcct
     2581 aacggcagga ggaccagtgt tgcgctggcg agggcagcga gttggagagg agagacagat
     2641 gatcggtgta tgccaatcac tgggtgctcg cggagttacc gcatccctac ctgttcgata
     2701 agcagcaggg taggctctcg gagttctgcg agcttcgcca aaggtacgag agccgcacgg
     2761 aagctttttc gttcgataag ggcgggatcc gccttgacct caggatggag tgggtactcc
     2821 ttgtcgaggt cggcgaacat cttctggccg gcttgtgcca cgagaaattc cacgagcaat
     2881 ttggcgttct ccacgcgggg actgtgcttc agaacgccga tgccggccac gttcatgatg
     2941 gcgcccatgc ctccttcctg ttgatcgggc atcaacaccg ccagcggagc agcgggttgc
     3001 gtcgcgagat gccggtacac atagtagtga ttcacgatgc ccatggcgac ctgacccttg
     3061 gcgacggcgt cgacgatctg cgaactcttc tgataaacct gggtgccggc attatcc
//
LOCUS       DATMTI010000981         5811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_503873, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000981 DATMTI010000000
VERSION     DATMTI010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503873"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..268)
                     /locus_tag="VN039_15515"
     CDS             complement(101..268)
                     /locus_tag="VN039_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47405.1"
                     /translation="MMGLTGHRLANKASTGTVTAPQNLVKVVFPQKTVVHSMQLIDSM
                     GRPTYRKDIHV"
     gene            complement(265..822)
                     /locus_tag="VN039_15520"
     CDS             complement(265..822)
                     /locus_tag="VN039_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47406.1"
                     /translation="MSDIGGTLKTKAGPLPVWAWTLLGTIALALFLIRKKSQSASTQA
                     AADQTNSDLGSAAQLANMFEVAGLMPYQGGDVYVNTTNTPPPAKTTLPAPTGNKHVGH
                     DIETSHFYKTTGVTDWNALAKKLGVFGGNGQALYEYNLIPNKHSPATLKAFKRTPKTV
                     SKGELIAVPMSGVLIDLPNVGTVKS"
     gene            complement(819..1400)
                     /locus_tag="VN039_15525"
     CDS             complement(819..1400)
                     /locus_tag="VN039_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47407.1"
                     /translation="MAPSPIVPSSDMYAARANASLDVYNAEMEPPATSYVTSTSPMSG
                     RYAPPVIAPKVNAPFVSHKPRMNGLIFNWAWGGNDMGAMTKSLPGVSGGLNGYVRSTN
                     FQTTLVQLHDWQTNANWYIAWNGTGAGMFQHQKQERYSYPSFRVSQIQTNVTGGVGQP
                     SMRMNRAPRFTSVQAIRKYTAQPSYYNTRSSKS"
     gene            complement(1521..1676)
                     /locus_tag="VN039_15530"
     CDS             complement(1521..1676)
                     /locus_tag="VN039_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47408.1"
                     /translation="MGAKVVNLFVIIAAGVMLADLVANPKGTAAFFNGIGSIWKTSVN
                     GMLGKTG"
     gene            complement(1676..1873)
                     /locus_tag="VN039_15535"
     CDS             complement(1676..1873)
                     /locus_tag="VN039_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47409.1"
                     /translation="MTGDKVFHLILVVVAGVIVADLIAHASGTSTLLSGFNTLFTIGT
                     QPTKTSAIKVTPVTNTGSRKA"
     gene            complement(1870..2250)
                     /locus_tag="VN039_15540"
     CDS             complement(1870..2250)
                     /locus_tag="VN039_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47410.1"
                     /translation="MSDKTVLGCFIGVLALIMYRDFKQPDKNWPLGPVPPPYRFTFAG
                     VVFGILFLVADIFNAKIAGVVAIGVLIGMMFSVTSGQSSTGGLKSNAPGYKTGGVASA
                     NPAANTGISGATPVTGSSGGSMMV"
     gene            complement(2875..3276)
                     /locus_tag="VN039_15545"
     CDS             complement(2875..3276)
                     /locus_tag="VN039_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47411.1"
                     /translation="MVYGPVAANIAATNTGTTEAVIATTPTISSAPAGGAGYSVRGVV
                     TFTGNASASTCTLKVRQGSNTTSGTAVYTSPAITVAAGAVVSIPFACLDLTAAGTGVA
                     NYSVTQTNSGGAGNSGTIVGYVEVMPAVEND"
     gene            complement(3276..3464)
                     /locus_tag="VN039_15550"
     CDS             complement(3276..3464)
                     /locus_tag="VN039_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47412.1"
                     /translation="MNAEAGVYTQAQLNTSLNALNGNPGQVSGTPAAWSYIWFGLAVL
                     FILVTYFGFGGLRGQVAS"
     gene            complement(3478..4569)
                     /locus_tag="VN039_15555"
     CDS             complement(3478..4569)
                     /locus_tag="VN039_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47413.1"
                     /translation="MPQTTGQPAAPGSTPFTRVSRQGQILGTSLAGQAYGSLWTPTLK
                     PVGGYLRHMILTVTSVTGGTLTSASAVADAPYNVIQNLFLRDPFGQPVLQASGYSLYL
                     INRYGGQHGMLGFGNDSTQLPSYQAPNNSTGAFNFALHLPLEMDSSGYCSLPSMNAAS
                     QPQLQIQWNPSATVFGTFTGTAPTITSAVNEPFWMAPVDQPQIAPPDVGSSAQWSEAR
                     AAAGVASSQFQRIQLPRVGTFIHTLILVLRDSTGARVEAWPSSDLTLWVDGVPILFET
                     LQERTDKLFAQFGANRPTGVIVYSFRDSVQNAVSTADTYDLLLPTTPATLLEVSGTWG
                     TISNAPAILTAVTGELYPVGGIPYTHLAN"
     gene            complement(4643..4783)
                     /locus_tag="VN039_15560"
     CDS             complement(4643..4783)
                     /locus_tag="VN039_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47414.1"
                     /translation="MENHVHIAGFFLLSVLAAEIFWHFLIRGIAAHHAESPAAQAIAN
                     LV"
     gene            complement(4871..5080)
                     /locus_tag="VN039_15565"
     CDS             complement(4871..5080)
                     /locus_tag="VN039_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47415.1"
                     /translation="MQIDENDARLISRAFVVLNEKLVAEYEKIQNTPDVSQLLVEAAH
                     ANYNETIKAYSDLHVKLVEAFPQLR"
     gene            complement(5297..>5811)
                     /locus_tag="VN039_15570"
     CDS             complement(5297..>5811)
                     /locus_tag="VN039_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47416.1"
                     /translation="PVDKIPSKFWRSFNDWLSTPKPREHWYRLVVREDWTDARAQVGK
                     ALAQCYKEHNWTIVLDETRALTDPRPPGLNLSPLVEQLWLRGGSKGINVVAATQGPRW
                     VPRSFYEQAQFHWIGRIEDEDSQKRLREIGGMERYHLPTIKGLQRHHFIYTDNLDDDG
                     VRYRGITKVE"
BASE COUNT         1333 a         1619 c         1571 g         1288 t
ORIGIN      
        1 ctgcgattga gcattgcttt cgttaccgct cttgttcttc aaatagaatc cgacggccac
       61 agccaccaca gcgataacac ctatgacaat ccactgctgc ttagacatgg atgtccttcc
      121 gataggtggg acgccccatg ctgtcaataa gctgcatgct gtgaacgaca gtcttctgag
      181 ggaagacaac cttcactagg ttctgtggag ccgtcacggt ccccgtagac gctttgttgg
      241 ccagtcggtg gcccgtaagt cccatcatga cttaaccgtc cctacgttgg gcagatcaat
      301 gagaacaccg gacatgggta cagcgatgag ttcacccttg cttacggtct tgggagtccg
      361 cttgaaagcc ttaagtgttg cggggctgtg cttgttcgga atcaggttgt actcatacag
      421 agcctgtcca ttaccgccga acacgccaag tttcttagcg agagcattcc agtcggtgac
      481 accggtcgtc ttgtagaagt gcgatgtttc aatgtcgtgt ccgacgtgct tattgccggt
      541 aggcgcaggc aatgtcgtct ttgccggggg cggagtgttc gtcgtattca cgtacacatc
      601 gccaccctga tatggcatga gacccgctac ttcaaacatg ttggccaact gtgctgcgct
      661 gcccaaatca ctgttcgtct gatctgcggc agcctgcgta cttgccgatt ggctcttctt
      721 acgaatcagg aacagggcaa gtgcgatagt accgagaagc gtccatgccc acacgggcaa
      781 cgggcccgcc ttagtcttga gagtccctcc gatatcgctc atgacttact cgaccgggtg
      841 ttgtagtaac taggctgcgc agtgtacttg cgaatggcct gaacactcgt gaagcgtgga
      901 gcgcgattca ttcgcatgga aggctgtcca acaccgccag taacgttcgt ctgaatctgg
      961 gagacccgga acgacgggta cgaatagcgt tcctgctttt ggtgctggaa cattccagca
     1021 ccggtaccat tccacgcgat ataccaattg gcgttggtct gccaatcgtg aagctgtaca
     1081 agagtggtct gaaaattggt gctgcggaca tatccattga gtccaccgct gacacccggc
     1141 aatgacttgg tcatcgctcc catgtcgttg ccaccccatg cccagttgaa aatcaggcca
     1201 ttcatgcgcg gcttgtgcga gacgaaaggt gcgttcacct tcggcgcaat aacgggtggt
     1261 gcataccggc cagacatcgg tgacgtgctg gtgacgtaag aggtggccgg gggctccatt
     1321 tccgcgttat agacatctag tgatgcattg gcgcgcgcag cgtacatatc gctgctaggc
     1381 acaatgggtg atggagccat gttccggccc ctcttacgac caaacatgat tactcacatt
     1441 ccggtagcgt gcttcttgcc gacgtagtaa ccgagggctg cggcgataac gagaagaact
     1501 acaagctgtc ccatgagaat tcacccagtc tttccgagca tgccgttgac gctggtcttc
     1561 caaatgctac cgattccgtt gaagaatgcg gcagtcccct tgggattcgc caccaaatcg
     1621 gcaagcatga cacctgctgc gatgatgacg aaaaggttca ccacctttgc acccattagg
     1681 ctttcctact tcctgtattg gtgactggtg tcaccttgat tgcacttgtt ttggtgggct
     1741 gcgtgccgat agtaaacaga gtgttgaagc ctgacaggag tgtgcttgtt cccgatgcgt
     1801 gggcaatcag atcggcgacg attacaccag ccaccacaac aaggatcaga tggaagactt
     1861 tgtcacctgt cataccatca tcgaccctcc gctagaaccg gtaacgggtg tggctccgct
     1921 gataccggta ttggctgcgg gattggcgct tgctactccg ccagtcttgt agccaggggc
     1981 gttggacttg agtcctcctg tagagctctg cccggacgtg acagaaaaca tcattccaat
     2041 gagcacgccg atagcgacaa cgccagcaat ctttgcgttg aagatgtctg caacaagaaa
     2101 cagaattcca aacacgacac ctgcaaaggt gaaccggtag ggcggtggca cgggacccag
     2161 tggccagttc ttgtcaggct gtttaaagtc ccggtacatg atgagtgcta gcacaccgat
     2221 aaagcatcca agaacggtct tgtcagacat cgtcacctcc cttaaggaat gaactttcct
     2281 gcccagtcgc cgaggctgcc tagaatgcct gacagtcccc cgagaaggga tcccccgcca
     2341 ctactgccac tgtcgccacc gctcttgccg ttgccaccaa tgttgggtgc tgtgctgaac
     2401 tgattagcgg tccccttgtt gagcagagcc tgtaggaagc cccacccttg tcctcccgta
     2461 aacagattgg aagcaccgga atagaacaga gcgtatccaa ctatcactag tgctattccg
     2521 aatgctttca ttagtgccac ctggaaatca tgtcagcgaa gccgtaaatc cctattcccg
     2581 ctgcgacggt tgtcgagtag gttcttgccc attcgcggta cgccgtagca ctctgtgatg
     2641 cagcaagcca ggcattccta ctagtcctgc gccaacttcc accactatcg gatccggcat
     2701 tggtgtggac agttctttta tcaggagacc gatcccgacg agaaatagga tgctgtccct
     2761 tattgcgaga atccacactc agtcacccca tgcggcgatt cttgctttca taccagacca
     2821 ctgcgagcag aataccgaga agaacaccaa tggaaaactt cacgttgaca ctcatcagtc
     2881 attctccacc gcaggcataa cttccacata cccgacgatg gttccggagt taccagcacc
     2941 tcccgagttg gtctgcgtca ccgaatagtt cgcgacgcca gtgccagcgg cagtcagatc
     3001 gagacaggca aaggggatgg acacaactgc acccgcagcc acggtaatgg ccggggaggt
     3061 gtagacagcg gtgcccgatg tcgtgttgct tccctggcgg accttaaggg tgcaggtgct
     3121 ggcggaagcg ttacccgtga acgtgaccac gccacgaacg gaataaccag caccaccagc
     3181 gggagcactg ctgatagtgg gagtggttgc aataaccgct tccgtcgtac ctgtattggt
     3241 cgcggcaatg tttgccgcta caggaccgta aaccattacg atgccacctg ccctcgaagg
     3301 ccaccaaaac caaagtaagt aacaaggata aagagaactg ccagaccgaa ccagatgtaa
     3361 gaccatgcgg cgggagttcc ggacacctgt cccggatttc cgttcaacgc attgagcgac
     3421 gtattgagct gcgcttgagt gtaaacaccg gcttcagcat tcatgtcacg tcacctttca
     3481 gttagcaagg tgggtgtagg gaatgccgcc aaccggatac agttcgccgg taaccgccgt
     3541 aagaatagcg ggcgcattgg aaatggttcc ccacgtaccc gacacttcaa gcagcgttgc
     3601 aggagtcgtc ggcaacagca ggtcgtacgt gtctgccgta gacacagcat tctggacaga
     3661 gtcgcggaag ctgtagacaa taacgcccgt cggacggttg gcaccaaact gagcaaacag
     3721 cttgtcagtg cgttcctgaa gagtttcaaa aaggatcgga acaccgtcga cccacagagt
     3781 gagatcgctc gacggccatg cctctacacg tgctccggtc gagtcccgaa gaacaagaat
     3841 cagcgtgtga atgaacgtgc cgacgcgggg aagctggatg cgctggaatt gcgaggatgc
     3901 gacgccagcg gcagcacgtg cctcactcca ctgcgcggaa gagccgacat ccggcggggc
     3961 gatttgaggc tggtcgactg gagccatcca gaatggctca ttgaccgcac tcgtaatggt
     4021 cggtgcagta cctgtaaacg tgccgaatac cgtcgcactg ggattccact gaatctgtag
     4081 ctgcggctgc gatgctgcgt tcatcgacgg cagtgaacaa tagcccgacg aatccatttc
     4141 gagcggcagg tgaagcgcga aattgaaagc accggtcgag ttgttcggag cctgatacga
     4201 gggaagctgc gtcgagtcat taccgaaacc cagcatgcca tgctgcccac cgtaccggtt
     4261 gatcaggtac agcgaataac ctgacgcctg caagacaggc tgaccgaacg ggtcccggag
     4321 aaacaggttc tgaataacat tgtacggggc atcggcgaca gcggaagcac tggtaagagt
     4381 cccgccggta accgacgtga ctgtcagaat catgtggcgc aggtacccgc cgacaggctt
     4441 cagcgtggga gtccacagag aaccgtaagc ctgccctgcg agagaagttc ccagaatctg
     4501 cccctgccgc gacacacgcg taaagggagt agaccccgga gcagcgggct gccccgtagt
     4561 ctgcggcatt cgagaaaccg tccccatgga cggattggca gtctgaagag ttgcgaccgg
     4621 agtaggcaac gtaattcctt tctcagacca ggtttgcgat cgcctgggct gcaggcgatt
     4681 cggcgtgatg tgcagcaatt ccacggatca ggaagtgcca gaagatttca gcggcaagta
     4741 cgctgagaag gaagaatcca gcaatgtgga cgtggttttc catggccacc ctttcaaaac
     4801 gtgaataggg ccatacaggc agtgtaccgc ctgtatggcc ctattcgtca acagtttgtt
     4861 gacccggatc ctaccggagc tgcgggaatg cctcgaccag cttcacgtgg agatcggagt
     4921 atgccttaat cgtttcgttg tagttcgcgt gtgccgcttc tacgagaagc tgtgaaacgt
     4981 ccggcgtgtt ctgaatcttc tcgtattccg ccactagctt ttcgttcagc acaacgaaag
     5041 cgcgggaaat cagcctggca tcattctcgt caatctgcat tcttaccatc cgttatcgaa
     5101 agtcggggct tgacttcatt actgtcggac aggttgcaac acggtccgat gtgacgtgtc
     5161 attgccccat gattgtggga gttgttgtaa atctcgcggt ccccacggcc gtcccactgt
     5221 tcggtgaatg cacgccatcc cttctggttc accgaaaaca tagtgccgca gttgtcacac
     5281 atcaatacag cgctcattac tcgacctttg ttattcctcg gtacctgaca ccgtcgtcat
     5341 ccaagttgtc tgtgtaaatg aaatgatgcc gttgcaaccc cttgatggtc ggaagatggt
     5401 acctttccat accaccaatt tccctgagtc gcttttgaga atcctcgtct tcaatgcgac
     5461 cgatccagtg gaattgcgcc tgttcataaa atgaacgcgg cacccatctc ggtccttgtg
     5521 tggcagcaac cacgttgatg cctttggagc caccacgtag ccacaactgt tcgaccaatg
     5581 gagataggtt gagccccggc ggacgagggt ccgtgagagc gcgagtctca tcaaggacaa
     5641 tcgtccagtt gtgctccttg taacactgcg ccaacgcttt tccgacttgc gcacgcgcat
     5701 ccgtccagtc ttctcttacc acaagccgat accagtgctc acgtggctta ggagtggaaa
     5761 gccagtcatt aaagctccgc cagaacttgg acggaatctt gtctaccggc t
//
LOCUS       DATMTI010000982         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_36231, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000982 DATMTI010000000
VERSION     DATMTI010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36231"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..1163)
                     /locus_tag="VN039_15575"
     CDS             complement(384..1163)
                     /locus_tag="VN039_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47417.1"
                     /translation="MSRDDPDRSKSSRAGTRRLIEQTPKALAGLAAMSGVAYLAGSIY
                     TKAYFSEFGASWILEEVPAATYFSQSWVPLLLMLYFGYLATTNLAMIGSQDDVTAGFQ
                     FRFSVALVRYGSWFLLALLAMTPLLSAFGYVVSAIVLSIIGVAAILLLFSSTLELVVA
                     RFNTMGRLIDLSMVYVAFAVIAAGLYVVPAQLGLNWARIDKRPTSSLLTVYLHSDAEK
                     EYRLLFSVGERLYVFPARFEGAYPTVQATAIANVGFVPPER"
     gene            complement(1278..1676)
                     /locus_tag="VN039_15580"
     CDS             complement(1278..1676)
                     /locus_tag="VN039_15580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWV47418.1"
                     /translation="MGQVLRIKTTAAKEIVDLTDRLETVIREAKLQDGLCALFVTHTT
                     AALTTGEIGEGTDDDLLEVVEEMIPRIRFQHAHNPAHAWSHMASSILGPSLTVPVSAG
                     KLVLGTWQSVMLIELDGPRERTVHVTVIPG"
BASE COUNT          416 a          476 c          479 g          331 t
ORIGIN      
        1 atagtcctga aacatgaaga gatcattagg attagatgaa aacttcttca taaatcataa
       61 aagtcgtaca tcaccgtcac tgcgcagcac ggaaggaccg ggacagcaag gccgcagcag
      121 cggggaggcg aagcatactt tgggccgtac gttgagcctc tgagttcacg ccgtgccgaa
      181 taaggcacgg cacgcttgtg aacgccgccg agatggtgag gcggctgtgt cttgcgagaa
      241 caccgtcggc gtatttttca ccagcttgct agtcggcgca ggtgcagtcc ggaaggacct
      301 cggtggcaat ggaggtgaga ttgttggtat aggagacaaa ccctttccgg cgtccaaaga
      361 gcgtcagcac acagtctgcc cccttaccgt tcagggggta cgaagccgac gttggcgatg
      421 gcagtggctt ggacagtggg ataggcccct tcgaatctcg cgggaaacac gtacaaacgc
      481 tccccaaccg agaatagaag tcgatactct ttttcggcat cactgtgcag atacacggtg
      541 agcagcgagg atgtcggtcg cttatcgatc cgtgcccaat tcaggccgag ctgcgcaggg
      601 accacataga gaccggcggc aatcacggcg aatgcgacat ataccatcga cagatcgatg
      661 agccggccca tggtattgaa ccgcgccaca accagctcga gtgtcgagct gaacaagagc
      721 aggatcgcgg cgacaccaat aatcgacaga acgatagctg acacgacgta gccgaatgcg
      781 ctcaggagcg gagtcatcgc taaaagcgcg agtaaaaacc atgaaccgta ccgaaccaag
      841 gccacggaga acctgaactg aaaacccgca gtcacgtcgt cttgactccc gatcatcgcg
      901 aggttcgtag tagccagata cccgaagtag agcatcagca acagcgggac ccagctttga
      961 ctgaaatagg ttgccgctgg aacctcttct aagatccacg aagctccaaa ttcagaaaaa
     1021 taggctttcg tgtaaatcga tccggcgaga taggccactc cgctcatggc ggccaatccg
     1081 gctaatgcct tcggcgtctg ctcgatgagt cgtcgagttc cggctcggga agatttgctt
     1141 ctgtctgggt cgtcacggga catgggtcgc gatgggaata ccgaaacggt cggtcattct
     1201 tgcttgtctt cggcatcacc cagtgtagat caggctactc ttccaggcgg ccccgacaca
     1261 agcgcctcat tctcgcctca cccggggatc acggtgacgt gcacggtccg ctcgcgcggg
     1321 ccatcgagtt cgatcagcat taccgattgc cacgtgccga gtaccagttt cccggccgat
     1381 accggcactg tcagagacgg tcccagaatt gacgaggcca tatgggacca cgcatgtgcg
     1441 ggattatgcg catgttgaaa gcgaatccga ggaatcatct cctcgacgac ctccaggaga
     1501 tcatcgtcag tcccctcccc gatttccccc gttgtcaacg cagcagtcgt gtgtgtcaca
     1561 aaaagggcgc aaaggccatc ctgcagcttc gcctcccgga tcaccgtctc aagccgatcg
     1621 gtcagatcga cgatctcttt cgcggcggtt gttttgatac ggaggacttg gcccatagga
     1681 aatggatctc aggaaagaaa aa
//
LOCUS       DATMTI010000983         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_376978, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000983 DATMTI010000000
VERSION     DATMTI010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376978"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VN039_15585"
     CDS             <1..1167
                     /locus_tag="VN039_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47419.1"
                     /translation="WWPINIHRLIANVVLGGYICGAYAGVRYLSVKSNEDREHYDWMG
                     YVGNFIGVFGLLALPFAGYWLMREIYQYNQQMGITLMGGFLSWLFIIQAMLIGVLFLG
                     SNYYFWLGITYRIPGSEAKYRRAMLLMLISLLLCLAVWMTPHSLVASIEEAQKMGGAH
                     HPLLGVLGVMSAKMTVSNLMILITFMSFVMYWRAGKQDTAVWAKLGKAVMGAVLVLAS
                     LAVIVLGVWGYFVPAIVRINYFSTSQVLIVIFVILTITPLTALLLKSARTTTEMVWGS
                     MPPRAGYALVLNAVMVILLMTLMGYARSSSRVHWHVYGVMRDSSPYAYSPALGSASLM
                     MGFCTFFFCILVAFIFWVATMGDKTKAAAASGKGDLPHGIPAMAGGAPEERTHE"
     gene            1160..1786
                     /locus_tag="VN039_15590"
     CDS             1160..1786
                     /locus_tag="VN039_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWV47420.1"
                     /translation="MSEVAQLQLIALSIVGVGILILLFIKATFVRVTGFVAIVLGLFS
                     LTCLAVPQMASLPPAEEKIDIASIKTPTDIAAIGQTVFFSKGQCALCHSIGPSESARC
                     PDLKGIGAKLSRDFLYESLTDPQAFVYHDYRHGGVPKEYPATMPAINKDPIGLSKNEI
                     FAVIAFLQQMSGEPISIGVADLEIPGKAPSAPVKAEESSLIADASRNQ"
     gene            1829..>2338
                     /locus_tag="VN039_15595"
     CDS             1829..>2338
                     /locus_tag="VN039_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWV47421.1"
                     /translation="MGALGKPIILMVTMYLFLKFVLPNIPGSAPLPSSLIFLYLLLTA
                     SGIIIYETLSGESKDAFWGPMQRFLTGEKIGGLQALRYGVLILFPLLVGWQTYGSTAT
                     SDLPPTEGRTIHPAPPGEYTGLSNPVSKTPENIMQGKGFYAAFCSPCHGGTFDGKGPA
                     ARGFNPPPAN"
BASE COUNT          451 a          643 c          678 g          566 t
ORIGIN      
        1 tggtggccga tcaacatcca tcggctcatt gccaacgtgg tgctcggcgg gtacatttgc
       61 ggcgcctatg ccggggtccg gtatctgtcg gtcaaaagta acgaggatcg agagcattac
      121 gactggatgg ggtacgtcgg aaattttatc ggcgtgttcg gcttgttggc tctcccattt
      181 gccggctact ggctcatgcg ggaaatctat cagtacaacc agcagatggg catcaccctg
      241 atgggaggat tcctgtcctg gctcttcatc atccaagcca tgttgatcgg tgtcctgttt
      301 cttggttcga actattactt ctggttggga attacctatc gcattcccgg atcggaagca
      361 aaatatcggc gggccatgtt gctgatgctg atcagcctgc tgctctgtct cgcggtgtgg
      421 atgacgccgc actcactcgt ggccagcatc gaagaggctc agaaaatggg aggggcccat
      481 cacccgttgc tcggtgtcct gggagtcatg tcggccaaaa tgacggtgtc gaacctcatg
      541 attctcatta ccttcatgag cttcgtcatg tattggcggg cagggaaaca agatacagcc
      601 gtatgggcta agctgggaaa agcggtcatg ggcgcggtgt tggtgctggc gagcctcgcc
      661 gtcatcgttc tcggtgtgtg gggatacttc gtgccggcca tcgtccgtat caattatttc
      721 tccacgtccc aggtgttgat cgtcatcttc gtcattctga ccatcacgcc gttgaccgcg
      781 ctgttgctca agagtgccag gacgacgacg gagatggtct ggggcagtat gcctccgcgc
      841 gccgggtatg ccctggttct caatgccgtc atggtcattc tcctcatgac attgatgggc
      901 tacgcgcgat cctcgtcccg cgtccattgg catgtgtacg gcgtgatgcg tgattcgtcg
      961 ccctatgcct attcgccggc cctcggcagc gcctcgctga tgatgggatt ctgtacgttt
     1021 ttcttctgca tcctcgtggc gtttatcttc tgggtcgcca ctatgggtga caagactaag
     1081 gcggctgcgg catcagggaa aggcgacctg ccgcacggca ttccggcgat ggccggaggg
     1141 gcgccggagg agaggaccca tgagtgaagt cgcacaacta caattaatcg cactgtcgat
     1201 cgtcggggtc gggattctga tccttctttt catcaaagcc acctttgtgc gggtcacggg
     1261 gttcgtcgcc atcgtgcttg ggttgttttc gctcacctgc ctcgccgtgc cgcagatggc
     1321 gtccctgcct ccggcggagg aaaagatcga catcgcgagt atcaagaccc cgaccgacat
     1381 cgcagcaatt ggacagacgg tatttttcag taaaggccag tgcgcgctgt gccattccat
     1441 cggccccagc gaatccgccc gttgtcctga tttaaaagga atcggtgcca agttgagtcg
     1501 ggatttcctg tacgaaagtc tgacggatcc tcaggcgttc gtgtaccacg attatcgaca
     1561 tggaggggtg ccgaaggaat atcccgcgac gatgccggcg atcaataagg atccgatcgg
     1621 gctctcgaag aatgaaatct tcgccgtcat cgcatttctg cagcaaatga gcggcgagcc
     1681 gatctccatc ggcgtggcgg atctcgagat acctggcaag gcgccgtcgg ctccggtcaa
     1741 agcagaggaa tcttcgttga tcgccgacgc atcacggaat caatgagcac attgccagga
     1801 tgttctacat cctgctaggg aggaacgtat gggcgcgtta ggcaagccga tcatactcat
     1861 ggtaaccatg tatctgtttc tgaaatttgt gttgccgaat atacccggct ccgcgcctct
     1921 tccctccagc ctgatttttc tctatctcct cctgactgcg tccggcatca tcatctacga
     1981 gacactgagc ggagagtcca aagatgcctt ctgggggccg atgcagcggt tcttgaccgg
     2041 agagaaaatc ggaggcttgc aggctcttcg gtatggcgtc cttattctgt ttcccctact
     2101 ggtagggtgg cagacctacg ggagtaccgc cacgagtgac cttccgccga cggaaggtag
     2161 aacgattcat cctgccccac cgggagaata caccggcctc tcgaaccctg tgtccaagac
     2221 tccggaaaat atcatgcaag ggaaggggtt ctatgcggcc ttttgctcgc cctgtcatgg
     2281 cgggactttt gatggaaagg ggccggccgc tcgcggcttt aatcctcctc cggccaat
//
LOCUS       DATMTI010000984         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_461605, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000984 DATMTI010000000
VERSION     DATMTI010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461605"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VN039_15600"
     CDS             complement(<1..644)
                     /locus_tag="VN039_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47422.1"
                     /translation="MDISVLIDSLHPLEIKVLTTLGLRPPGTALDSEQLASAAELEPS
                     QLSMAVEWLLAKSLITVQTETVTPIVSLTKVGEEYYQTASPIERVLAAAREATGTGTR
                     LTIPDLQSQGGLDSSDVSKAVGRLKKEGVVLIIQGGCIETTGRQSPTAEALRTLLGEV
                     HDSPKELKGFTEAHQQVIEDFAVKRGNAKEPFRVDDRVARSFILSPDGAGATALL"
     gene            complement(655..1356)
                     /gene="rpe"
                     /locus_tag="VN039_15605"
     CDS             complement(655..1356)
                     /gene="rpe"
                     /locus_tag="VN039_15605"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWV47423.1"
                     /translation="MGHPIHIAPSILSADFARLADEVAAVERAGADLLHVDVMDGHFV
                     PNLTVGPPIVESLRKVTKLPLDVHLMITNADAFIPEFAEAGADYLTVHVEACPHLHRT
                     VQSIKERGVKAGVTLNPATPFSLLQEILGDVDLVLIMSVNPGFGGQTFIPSALKKITE
                     ARVMLDTINSHALLEVDGGVKVDNAREIVAAGATALVAGSAIFSQHDYTATIAAMRAA
                     AETVVPRAPHAAVNR"
     gene            complement(1356..>1841)
                     /locus_tag="VN039_15610"
     CDS             complement(1356..>1841)
                     /locus_tag="VN039_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47424.1"
                     /translation="ERLCLQSIVPIVGDVRKPSEWSGMVARESHLREEPLFDRILVDA
                     PCSGVGVLRRHPEAKWRKGSSAFARHRKLQGEILTSVACCLRPGGVLVYSTCSTEKEE
                     TEEIVDRFCQAYPGWMRESVAPWLPTPALPFVTIYGALSTMGNECGMDNFYAARLRKK
                     R"
BASE COUNT          378 a          555 c          511 g          397 t
ORIGIN      
        1 agcagcgccg ttgcaccggc accgtccggt gacaatataa aagagcgggc cacccgatca
       61 tccacccgga acggctcttt ggcattgcca cgtttcacgg caaagtcttc aatgacctgt
      121 tgatgggctt ccgtgaagcc cttcagttcc ttcggcgaat catgcacctc tcccaagagg
      181 gttcggagtg cctcagccgt cggactctgg cgcccggtcg tttcgataca acctccttgg
      241 atgatcagga ccaccccttc cttcttgagc cgtccaacgg ctttactgac atcggaagag
      301 tcgagccctc cttgtgattg aaggtcggga atggtcagtc gcgtcccagt gccggtcgct
      361 tcgcgggcag ccgccaagac tcgttcgatt ggtgaagcag tctggtaata ctcctcccca
      421 actttcgtca gtgatactat tggcgtcaca gtttccgtct gcactgtgat gagcgacttc
      481 gccaacagcc attcgaccgc catgcttaat tgagaaggct ccagctcagc ggcactcgcc
      541 agctgttcgc tgtccaaggc agttccaggc ggacgaagcc ccaaggtcgt gagtacttta
      601 atctcgagag ggtgcaggct gtcgatgagt acggagatgt ccaccctaaa tggcctatcg
      661 attgaccgct gcgtgtggcg cccggggaac gaccgtctct gcggctgctc gcatggctgc
      721 gatcgtggcc gtatagtcgt gttgggagaa aatagcggac cctgccacga gagccgttgc
      781 accggcggct acgatctccc tggcattgtc gaccttcacc ccgccatcca cttcgagcag
      841 cgcatggctg ttgatcgtat ctaacatgac ccgcgcttcg gtgattttct tgagtgccga
      901 tgggatgaat gtctgcccgc caaaccccgg attcaccgac atgatcaaca caagatcaac
      961 atcccccaag atctcttgga gcagcgagaa tggagtggcg ggatttaagg tcactccggc
     1021 cttgacccct cgttccttaa tcgattggac cgtccggtga aggtgtgggc aggcctcaac
     1081 gtggacggtg agatagtcgg cgcccgcttc ggcaaactca gggatgaacg catcggcgtt
     1141 ggtgatcatc agatgaacgt cgagcggcag cttcgtgact tttctcaggc tttcgacgat
     1201 gggtggcccc accgtcaggt tcggcacgaa atggccgtcc atgacatcta cgtggagcag
     1261 gtctgcgccc gcccgctcca ccgctgctac ttcgtcggcc aatcgcgcga agtccgcgga
     1321 aagaatcgag ggcgcgatat gaattggatg acccattacc tcttcttccg taggcgggcg
     1381 gcataaaagt tatccatccc gcattcgtta cccatggtgg agagcgcgcc gtagatggtc
     1441 acaaaaggga gagcaggggt ggggagccac ggcgccacag attcacgcat ccatccagga
     1501 taggcttggc aaaaacggtc gacgatctct tccgtctctt ccttttctgt cgagcaggta
     1561 ctatagacca gcaccccacc gggtcgcaag cagcacgcca ctgaagtaag gatttcgccc
     1621 tgaagtttcc ggtgcctggc gaacgccgaa ctgcccttcc gccatttcgc ttcagggtgt
     1681 cggcgcaaca cacccacgcc actgcaaggg gcatccacga gtatacgatc gaacagtggc
     1741 tcctcacgaa gatgcgactc gcgtgcaacc attccagacc attcggatgg ttttctcaca
     1801 tcgccgacga tcggcacgat gctctgaagg cacaatcttt c
//
LOCUS       DATMTI010000985         1562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_250532, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000985 DATMTI010000000
VERSION     DATMTI010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250532"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="VN039_15615"
     CDS             <1..1480
                     /locus_tag="VN039_15615"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWV47425.1"
                     /translation="LFGKDAAMEGKMYVRFQNSKETYLASQSVRKDIDKKPEEFRDRK
                     LTDLSNAQVRRVVLKTPAGEIELEKKDTHWEILKPLRARADDQKVNDLISQVTSARIG
                     QFIADDRGDLRPYGLAEPLGAISLFDQEQKRDQKVEIGGSIKVAGREDKGQTLQIGAT
                     PEKEKDEVYVRFTPRGSVYTLPKKIEEILNTKPADLRDNHLVRIDTNILDRITIDAPG
                     KTKTVLARKDGNWTIANRNNEVADSGAVRRMIDALQNERVTRFVEDVASNLPKYGLDK
                     PSLQVTFSSFASENTAESKAGEEPFATITFGKEEGDQVFARLTDEPFVVAVRRALLDQ
                     ISADPLRWQDLSVFKFKPEQIHMVSITTDRELALERDANNQWHWLKGSGAINQPNLQA
                     ILKTLSNLYAVRWLGPPTPQQGLDKPQLTVSFSTSPDNKTTGKLIVGAQNNDGTFFAR
                     VEGRDGTFAISGPDFIKLKLPLETQGAVSPSPAATSSPGTTP"
BASE COUNT          419 a          411 c          435 g          297 t
ORIGIN      
        1 cttgtttggc aaagacgctg caatggaagg caagatgtat gtgcgattcc aaaactcgaa
       61 ggaaacctat ctggccagcc aatcagtacg gaaagacatc gacaaaaagc cggaagaatt
      121 ccgggaccga aaactgacgg atttgagtaa cgcgcaggtg cgccgcgtcg tgttgaaaac
      181 gcccgccggt gaaatcgagc tggagaagaa agatacgcat tgggaaattc tcaagccgct
      241 ccgcgcacgc gccgacgatc aaaaggttaa tgacctgatc tcgcaggtca catccgcgcg
      301 cattggacaa tttatagctg acgatcgcgg cgacctgcgt ccttacggat tagcggaacc
      361 gctcggagcg atctcgttgt tcgatcagga gcagaaaaga gatcagaaag tcgagatcgg
      421 cggatctatc aaagtcgccg gccgggaaga caaaggccag acgctgcaaa tcggcgcaac
      481 accggaaaag gaaaaggacg aggtatatgt gcggttcact ccacggggtt ctgtctacac
      541 gttgccgaaa aagatcgaag agattctcaa caccaagccg gcggacttgc gcgacaatca
      601 tttggtccgg atcgacacca atattcttga tcggatcacg atcgatgcgc ccggtaaaac
      661 caaaaccgta cttgcacgca aagatggaaa ctggacaatc gcaaatcgaa acaacgaagt
      721 agcagattca ggagcggttc ggcgcatgat cgatgcgctt cagaatgagc gggtgacacg
      781 attcgtcgaa gatgtcgctt ccaatctgcc caagtacggt ctcgataagc cgtcgcttca
      841 ggtaacgttc agttcgtttg cctcggagaa caccgccgaa tcgaaagcag gcgaggaacc
      901 gttcgcgaca attacatttg gaaaagagga gggggaccag gtcttcgcgc gtctgaccga
      961 tgagccattt gtcgtggcag tacgtcgcgc gttactcgat cagatttcag ctgatccgct
     1021 gcggtggcag gatttgtcgg tcttcaaatt taaacccgag cagatccata tggtaagtat
     1081 cacgaccgac agggagctgg cgttggaacg tgatgcaaac aaccagtggc attggctcaa
     1141 gggaagcggc gcaatcaatc agccaaattt gcaagcgatc ctcaaaacgc tgtcgaacct
     1201 ctatgcggtg cgctggcttg gaccaccaac acctcagcaa ggattagata aaccacagct
     1261 cacggtgagc ttctcgactt cacctgacaa caagacaacg gggaaattga tagtcggcgc
     1321 ccaaaacaac gacggcacat tctttgcacg cgtggaaggg cgcgacggca cgtttgcgat
     1381 cagcggcccc gacttcatca aactgaaatt gccgctcgaa acgcaaggcg cggtctcccc
     1441 ttccccggct gccacctcga gtccggggac aacaccctaa cgaatcatcg cttcgttctt
     1501 tgttgcgttt ttggcgatgg agatgggcga gcaggaggcc cgccgccgcc tgtgcggctg
     1561 ac
//
LOCUS       DATMTI010000986         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_588051, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000986 DATMTI010000000
VERSION     DATMTI010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588051"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VN039_15620"
     CDS             <1..627
                     /locus_tag="VN039_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV47426.1"
                     /translation="LLADDHTFVLEGFKKLLEDHCDLVGAVEDGRALIEDTLNLRPDV
                     VILDISMPRLNGIEAAKKLKKQIPEVKLIFVTMHADLAYVNAAFRAGASGYLLKRSAA
                     TELMQAVQFVMNDKFYVTPLITKEVVASFLKPTQPRLATIEDLTTRQHEILQLVAEGL
                     SAKEIADQLQISHRTVEFHKTKIMEQLNVHTTTELVKYAMAHGLVTTA"
     gene            951..>3185
                     /locus_tag="VN039_15625"
     CDS             951..>3185
                     /locus_tag="VN039_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV47427.1"
                     /translation="MIISRRQFLKASAGTVAAAAVADQVLALTALQPVIEVGNPLGDY
                     PDRSWERVYHDQYRYDSSFTWVCSPNDTHACRIRAFVRNGVVMRVEQNYDHQTYEDLY
                     GNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKQWMDDGSPELTPESKRKYK
                     FDSRFLDDMLRVSWDTAFTYAAKAMIIIGTRYSGEAGARRLREQGYAPEMIEMMKGAG
                     VRCFKHRAGMPILGFIGKHSNTRFNNSVLPVLDTWIRKVNPDQAQGGRYWNNYTWHGD
                     QDPSQPWWNGTQNCDTDLSDMRFSKLNTSWGKNFVENKMPEAHWKLESIERGARLVVI
                     TPEYNPTASRADYWIPLRPSSDASLFLGACKIILDENMQDIDYIKQFTDMPLLVRTDT
                     LQYLDPRDVIQNYKFPDFSHSYSGRIQALKPEYIERLGGFMVWDVAKKQAAPLHREQV
                     GWHFEKSGIDPALTGSYRVKLLNGREIDAMPIYQLYLIHLQDYDLDTVHQITRSPKDL
                     LVRWARDSGTIKPAAIHNGEGVCHYFHMTQTGRAAALVVTLTGNIGKFGTGCHTWSGN
                     YKVGIWNATPWSGVGSGVHLSEDPWQLNLDANAHGKEVKYRNYYYGEEPGYWNHGDTA
                     LIVNTPKYGRKVFTGKTHMPTPSKLRWVVNVNILNNAKHHYDMVRNVDPNIECLITQD
                     IEMTSDVNHNDIAFAANSWMEFTYPEMTATVSNPWIQLWKGGIRPLYDTRNDADTFAG
                     VAAKL"
BASE COUNT          731 a          900 c          911 g          643 t
ORIGIN      
        1 ctgttagctg acgaccatac ctttgtgctc gagggattca agaaattgct ggaagaccat
       61 tgcgacttgg taggagccgt agaagacggt cgcgccttaa tcgaagacac cctcaatctc
      121 cggccggacg tcgtgatatt ggacatttca atgcctcggc tgaacggcat cgaggcggca
      181 aagaagctca agaagcagat ccccgaagtg aaactgattt tcgtcacgat gcatgccgac
      241 ctggcctatg tcaacgccgc gttcagagca ggtgcatccg gctacttact gaaacgatcg
      301 gcggccaccg agttgatgca ggcggtccag ttcgtgatga acgacaagtt ctatgtgacc
      361 ccgctcatta ccaaagaagt tgtggcgtct ttccttaaac cgacacagcc ccgtttagcc
      421 accattgagg acttgaccac gaggcagcac gagattctgc aattggtggc cgaaggattg
      481 tccgcgaaag aaatcgccga ccaactgcag atttctcacc gtaccgttga atttcacaag
      541 accaagatca tggaacaact caacgtccac accacgacgg agctggtcaa atacgccatg
      601 gcgcatggct tagtcacaac agcgtgattc ccctgcgtac aatacggtag ttgcctcgct
      661 gaaacctcgt agtttctgta tgcacagccg gtaatctttc tttctctcct cccactacca
      721 ctccgctaca ctacatcatc gtgaaagtcg cacatcaaca ctccggtaaa tggggttacg
      781 ccctctcaac tcatcatcga gcaggcgaca gttaataacg tgaatgcatg tttatcgatc
      841 aaatggtgat ctcaggacat gggatgaagt caacaaagag caaggatgct gatctttctc
      901 atattctaac tgagtcttcc tcataactga actgaaggga ggtagagcac atgattatct
      961 cgcgtcgaca gtttttgaaa gcctctgcgg gcacggtggc ggccgcggcg gtggcggatc
     1021 aggtcctggc cttgacggcc ttgcaaccgg tgatcgaagt cggcaatccg ctgggggatt
     1081 atccggaccg ctcgtgggag cgggtctatc atgatcagta tcgttacgat tcctccttta
     1141 cctgggtgtg ctctcccaac gacacccatg cgtgccgcat ccgggccttc gtgcgcaacg
     1201 gcgtggtcat gcgggtcgag cagaactatg accaccaaac gtatgaagac ttgtacggca
     1261 atcgcgggac gttcgcgcac aatccccgga tgtgcttgaa agggtttacc ttccatcggc
     1321 gggtctatgg gccgtatcgg ttgaaggggc ccttgatgcg gaaggggtgg aagcagtgga
     1381 tggatgacgg ctcgccggag ctgacgccgg aaagcaagcg caagtacaag ttcgacagcc
     1441 gcttcttgga cgatatgttg cgggtgtcgt gggatacggc gttcacctat gcggcgaagg
     1501 cgatgatcat catcggcacg cggtacagcg gggaagccgg cgcgcggcgg ctgcgggagc
     1561 aaggctatgc cccggagatg atcgagatga tgaagggggc gggcgtgcgc tgtttcaagc
     1621 atcgtgccgg catgcccatt ctcggattca ttggtaaaca ttccaatacc cgttttaaca
     1681 acagcgtgct gccggtgctc gacacctgga tccgcaaggt caatccggac caggcgcaag
     1741 gggggcgcta ctggaacaat tacacctggc acggcgacca ggacccctcg cagccctggt
     1801 ggaacggcac gcaaaactgc gataccgatt tgagcgacat gcggttctcc aaattgaaca
     1861 cgagctgggg caagaacttc gtcgaaaata agatgccgga agcgcactgg aagctcgaat
     1921 cgatcgagcg tggtgcccgt ctcgtggtga tcaccccgga gtacaatccg acggcctcgc
     1981 gcgccgacta ctggattccc ctgcgcccgt catcggacgc ttcactgttt ctgggggcct
     2041 gcaagatcat tctcgatgag aacatgcagg acatcgacta catcaagcag tttacggata
     2101 tgccgctcct ggtccgcacg gatactctcc agtatctgga cccgcgggat gtgatccaga
     2161 actacaagtt cccggatttc tcccacagtt attccggccg gatccaagcc ttgaagccgg
     2221 agtacatcga acggctcggc gggttcatgg tgtgggatgt ggccaagaaa caagcggcgc
     2281 cgctccatcg cgagcaggtc ggctggcatt tcgagaagag cgggatcgat ccggccttga
     2341 ccggctccta tcgcgtcaag ttgctgaacg ggcgggaaat cgacgcgatg ccgatctacc
     2401 agctgtatct gattcacctc caagactacg atctggatac cgtgcaccag atcacccgct
     2461 cgcccaagga tctgctggtc cggtgggcgc gcgactcggg cacgatcaag ccggcggcga
     2521 tccacaacgg cgaaggggtc tgccactatt tccacatgac gcaaacgggc cgggcagcgg
     2581 ccctcgtggt gaccttgacc ggcaatatcg gcaagttcgg caccggctgt catacctggt
     2641 ccggcaatta caaagtgggg atttggaatg ccacgccctg gtcgggcgtc ggctccggcg
     2701 tgcatctgtc ggaagatccc tggcagctca atctggatgc caacgcccat gggaaggaag
     2761 tcaaataccg gaactactat tacggcgagg agcccgggta ctggaaccac ggcgacaccg
     2821 ccttgatcgt caatacgccg aagtatgggc gcaaagtgtt taccggcaag acccatatgc
     2881 ccacccccag taagctccgg tgggtcgtca acgtcaacat cctcaataac gccaagcacc
     2941 attacgacat ggtgcggaac gtcgatccga acatcgaatg cctcatcacg caggacatcg
     3001 aaatgacgtc cgacgtgaac cacaacgata tcgcctttgc cgcgaactcc tggatggagt
     3061 tcacctatcc ggagatgacg gccacggtgt ccaatccgtg gatccagctc tggaagggcg
     3121 gcatccggcc cttgtacgac acgcggaacg atgcggatac gtttgcgggc gtggcggcga
     3181 aactg
//
LOCUS       DATMTI010000987         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_844361, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000987 DATMTI010000000
VERSION     DATMTI010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844361"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VN039_15630"
     CDS             complement(<1..237)
                     /locus_tag="VN039_15630"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HWV47428.1"
                     /translation="MSIVTKTGDKGETSLMYGRRLSKADPRVEAYGCVDELSAALGLA
                     RSIAANDFISEQILTAQKDLIVVMGELATAPSDRE"
     gene            complement(318..>2381)
                     /locus_tag="VN039_15635"
     CDS             complement(318..>2381)
                     /locus_tag="VN039_15635"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWV47429.1"
                     /translation="IWTHAGDQISNVMFREIEENQDRKEFNPIHLMVDSGARGNRQQV
                     RQLAGMRGLMAKPSGEIIERPITSNFREGLTVLEYFISTHGARKGLADTALKTADSGY
                     LTRKLVDAAQDVIIVNDDCGTVNGIVVRSIYEGDEEVVDLGQRIIGRVSCETIADPVD
                     KKKKIVKANQYIDEKIAADLQRIGVEQLKIRSVLTCECGRGVCAMCYGRNLATGAFVK
                     LGEAVGIIAAQSIGEPGTQLTMRTFHIGGTASQTFKQPIIKAKNDGTVQFNDLRTVQA
                     LDGSWIVLNKNGSISVNNDEGRELERYNIVIGSVISKEDGATVKKGETFVQWDPYNVP
                     ILTDKGGKIEFRDMIAGVTIKREVDEATGLMGTMVIEHKEDLHPQVVIVDDKKEVLAS
                     YSIPAGAHVIVEEGQKVRAGALLAKTPRKVAKTKDITGGLPRVAELFEARRPKDAAEI
                     AKIDGIVELGGTVRGKRSLILKDPDTNAEEEHLIPLTKHIIVFKGDFVKKGQQLTEGP
                     IVPHEILEVCGPQELQEHLVNEVQEVYRLQGVEINDKHIEIIVRQMLRKVKITDPGDT
                     SLLWGDQVDKLDFEEENKKVVEKGGKPAEAVPVLLGITKASLETDSFISAASFQDTTR
                     VLTEAATLGKIDRLRGFKENVIMGHLIPAGTGFPEHRQLKLVEKGEPVGAPVMEEAEP
                     QPAIG"
BASE COUNT          471 a          700 c          605 g          605 t
ORIGIN      
        1 ttctcgatcg gacggcgccg tagccagttc ccccatcacg acgatcaaat ctttctgtgc
       61 tgtgagaatt tgttcggaaa taaaatcgtt ggctgcaatc gatctcgcca gaccaagtgc
      121 cgcgctcaac tcatcaacac atccataggc ctcgacgcgc ggatcggctt ttgatagccg
      181 tcgcccgtac atcagcgacg tttcgccttt gtcccctgtt ttagtgacga tggacacgcg
      241 aaaattgtag ggcgtttctg tgaaacgcca accgtaagat cgcgtccgcc gggaccgacg
      301 ccctacaaat catcgcacta accgatcgca ggttgcggct cggcttcttc catcactgga
      361 gcgccgaccg gctcaccttt ttccaccagt ttaagctggc gatgttcagg gaaaccggtt
      421 ccggcaggaa tcaggtgacc catgatcacg ttttccttga aaccacgcag tctgtcgatc
      481 ttgccgagtg tagccgcttc agtgagaaca cgggtcgtat cctggaatga agccgcagaa
      541 ataaagctgt cggtttccag ggaagctttg gtgatgccga gcaacaccgg aacagcctcc
      601 gcaggcttgc cgcctttctc aacgaccttc ttgttctctt cctcgaagtc gagtttatca
      661 acctgatcgc cccagagtaa cgacgtatcg ccgggatcgg tgatcttcac cttgcgcaac
      721 atttggcgca cgatgatctc gatatgctta tcgttgatct ccaccccctg gagccgatac
      781 acctcctgca cttcgttcac caggtgctcc tgcaattcct gcggaccgca cacttccaga
      841 atttcgtgcg gtacgattgg gccttcggtc agttgttgac ctttcttcac gaaatcgcct
      901 ttgaatacga tgatgtgctt ggtaagcgga atcagatgct cttcttcagc attggtatcc
      961 gggtctttca gaatcaaact gcgtttgccg cgcacggttc cacccagctc aacgatgccg
     1021 tcgattttgg caatctcagc cgcatctttt ggccggcgag cttcgaacag ttccgcaaca
     1081 cgcggcagac cgccagtgat atccttggtc ttggcgacct tacgcggagt tttagcaagc
     1141 aacgcgcctg cacggacctt ctggccttcc tccacgatga cgtgagcgcc agcgggaatc
     1201 gaatagctgg cgagcacttc cttcttgtcg tccacgatca ccacctgggg atgcagatct
     1261 tccttgtgct cgataaccat ggtgcccatg agaccggttg cttcatcgac ttcgcgtttg
     1321 atcgtcactc ccgcgatcat gtcgcggaat tcgatcttac cccctttgtc ggtaaggatc
     1381 ggcacgttgt aaggatccca ttgcacgaag gtttcaccct tcttcaccgt ggcgccgtct
     1441 tctttggaaa tcacagagcc gatcacgatg ttgtaacgtt ccagttcgcg tccctcatcg
     1501 ttgttcacgc tgatcgagcc gtttttattc agcacgatcc agcttccgtc gagtgcctgc
     1561 acggtgcgca aatcattgaa ctgaaccgtg ccgtcattct tggccttaat gataggctgc
     1621 ttaaaggtct ggctcgccgt tcctccgatg tggaaggtgc gcatcgtcaa ttgcgtcccc
     1681 ggctcaccga tggactgcgc tgcgatgata ccgacggctt caccgagctt cacgaaggcg
     1741 ccggtggcga gattgcgacc gtagcacatt gcacacacgc ctcgtccgca ctcgcacgta
     1801 agcaccgacc ggatcttcaa ttgttcgacg ccgatgcgtt gtaaatcggc cgcgatcttc
     1861 tcgtcgatgt attggtttgc cttgacgatc tttttctttt tgtcgaccgg atccgcgatt
     1921 gtttcgcagc tcacacgacc aatgatgcgc tggccgagat cgacgacttc ttcgtcacct
     1981 tcgtaaatcg agcgcaccac gattccgttt accgtaccgc agtcgtcgtt aacgataatg
     2041 acgtcttgcg cggcatccac gagtttgcgc gtgagataac cggagtcggc ggttttgagc
     2101 gcggtatcgg cgagaccttt gcgggcgccg tgagtagaaa tgaaatactc gagcacggtc
     2161 aatccttcgc ggaaattcga cgtaattgga cgctcgataa tttcgccgct tggcttggcc
     2221 atcaaacctc gcatgcctgc gagctgtcgc acttgctggc gattgccacg ggcgccggaa
     2281 tcgaccatca ggtgaatcgg gttgaactct ttgcgatctt gattttcctc gatctcacgg
     2341 aacatcacgt tggagatttg atcgcctgcg tgcgtccaga t
//
LOCUS       DATMTI010000988         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_250598, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000988 DATMTI010000000
VERSION     DATMTI010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250598"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VN039_15640"
     CDS             complement(<1..818)
                     /locus_tag="VN039_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWV47430.1"
                     /translation="MGVQAQFPGEQSPAGEFMRQPDAFRKWVTTDGSSGYPSASGRYH
                     LYVSWACPWAHRTIIVRKLKQLEDVIGMTVVDPIRDERGWAFREGSGHSLDRINGFQF
                     LREAYKATDPNYIGRMTVPALWDCVTKRIVTNSDDDLMRIFNGEFNRFTKSPIDLYPD
                     DLRQEIDELNTFIYENVNDGVYRAGFATSQQAYERAARRLFTALDQLEARLATRRYLF
                     GPEFVETDWRLFVTLVRFDAVYYGHFKCNVRRIIDYPHLFGYLRDLYQTDGIADT"
     gene            complement(1080..1532)
                     /locus_tag="VN039_15645"
     CDS             complement(1080..1532)
                     /locus_tag="VN039_15645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV47431.1"
                     /translation="MVHIRPATESDFPALLRVQQTAFGEYAGVYKVSGWTTETLESLK
                     EDAREKHIFVAEEGEAIVGSVRFWTVAGVCVIRLLSVSPAYQQQGVGKALIREIERAS
                     TDAHKFYACTMLRTARNIQFFMNLGYKAETILPDHYDHLDLICFAKYR"
     gene            complement(1906..2226)
                     /locus_tag="VN039_15650"
     CDS             complement(1906..2226)
                     /locus_tag="VN039_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47432.1"
                     /translation="MKQTTNPVNLRIEWCRKQCAQARTEPEVDGWHAEADGLRDALMN
                     TDHTDHYRLCPPEILRRYVLGFQDGTALLRAARVQRMSRAATSWMPLQASRMGGDVLR
                     GDDR"
BASE COUNT          605 a          672 c          689 g          606 t
ORIGIN      
        1 gtatcggcaa taccgtcggt ttggtagaga tctctgagat acccgaagag atgtggatag
       61 tcgatgattc gtcggacatt gcacttaaaa tgtccgtagt agaccgcatc gaaccgaacc
      121 aacgtgacaa agagacgcca gtcggtttcc acgaattccg gtccgaataa gtaacggcga
      181 gttgcgaggc gcgcctcgag ctgatccagc gcggtaaaca atcgccgcgc cgctcgttca
      241 tacgcctgtt gcgatgtggc gaatcctgct cgatagactc cgtcgttcac gttttcgtaa
      301 atgaatgtgt tgagctcgtc gatctcctgc cgcaggtcat ccgggtacaa atcgatcggg
      361 cttttggtga atcgattgaa ctcgccgttg aagatcctca tcagatcatc atcggagttg
      421 gtcacgattc gtttggtcac gcaatcccac agtgccggga cagtcatgcg cccgatgtag
      481 ttcggatcgg ttgccttata cgcttcacgg agaaactgaa agccgttgat ccgatcgaga
      541 gaatgtcctg aaccttcgcg aaatgcccat cctcgctcgt cacgtatagg atccacaacc
      601 gtcatgccga tcacatcttc aagctgtttc aacttgcgca cgatgatcgt ccggtgggcc
      661 cagggacagg cccatgagac gtagagatgg tatcggccgg aggcagaggg ataaccggag
      721 ctgccgtcgg ttgtgaccca tttcctgaac gcgtccggtt gtcgcatgaa ctcaccggcc
      781 ggcgattgct cgcctggaaa ttgagcttga actcccatgg agggtcgctt ccttcagaag
      841 cagtatctca tatctacaac caataatatt aaagaagtct cggtgcacga ccgcatttcg
      901 ccgcccgccg aaaggaatgg ggaaaaaggg agctgtgcct cggtgacgcg atctcgccac
      961 agcaaccatc cacttccaac cgtcatgaaa ggtcaggcac taaacttgtg cgtgcttgtt
     1021 ggtaagtctg cggggttgtg acacttgtgg ggcacggcga ggctgaatcc agacacagct
     1081 catcgatatt tcgcgaagca gatgaggtcg agatggtcat aatgatcggg aagaatcgtt
     1141 tcggccttgt agccgaggtt catgaagaac tgaatgtttc tcgccgtgcg cagcatggtg
     1201 caggcgtaaa atttgtgggc atcggtcgat gctcgttcga tctcgcggat cagagccttt
     1261 cccactcctt gctgttggta cgcggggctc acggaaagca atcggatcac acagacaccc
     1321 gccacagtcc agaagcgcac ggaaccaacg atcgcttccc cctcctccgc cacaaaaata
     1381 tgtttctccc ttgcgtcttc tttcaaactc tcgagagttt ccgtggtcca tccgctgacc
     1441 ttgtagacgc cggcgtattc accgaacgca gtctgctgta cccggaggag agcaggaaaa
     1501 tccgactcgg tggctggtcg aatgtgaacc acaacgcaga tccttggcta gtgactttca
     1561 taacggaagg aagggagatt cgcaagcatg gttgcctggg ctttgagagc aggcgatgag
     1621 atgcattact gaagagagtt acatcggtag acaaaattag ccggttaagg tgcgtacggc
     1681 ctgccggcag gccgtacatc cgtcacgcag cttcgcttcc gtaccggcga cgttcggagc
     1741 cggtcgacat tctctgccgt gagacaactt gtcgattatg attgattaca tcatcgacga
     1801 ccattccaca attgacacag cgccaaccat gaaagtccga tgacgagcca gtctgcaccg
     1861 agatgctgtc gtcactgacg gcaagaccgt tgcatcgtag gcaattcatc gatcatctcc
     1921 ccgtagaaca tcacctccca tccgagatgc ctgtagcggc atccacgatg tggcggcgcg
     1981 gctcatgcgc tgcactcgtg cggctcgcag caacgccgtc ccatcttgaa agcccagcac
     2041 gtaccgcctg aggatctcgg gggggcacag tctatagtgg tcggtgtggt cggtattcat
     2101 cagggcatcc cgcagtccat ctgcttctgc atgccacccg tccacctccg gttcggtgcg
     2161 agcctgtgcg cattgctttc gacaccattc aatccgaagg ttcacggggt tggtcgtttg
     2221 tttcatgttt cacccccttc ttaagtcctg tatatgcacc agcaaattga atgccaagag
     2281 aggaatacga acaagctgat tcttggttcc gctcgattgg atctagtagt tacatattgt
     2341 taatgactta tcagcaataa gcaatatcgt tgcccacaaa gagtgggcat agcagagcgt
     2401 tcgatgaact ggagaatgtg aaggatatat gatctactgt ttcctaatga tactagggac
     2461 aaccctagat cgatgtcggg gttggagaga atgtaggcgt gtggcatgga aaagaataaa
     2521 tacattttag tagctattat atcttagtat tagcggaatc gtttttacag aa
//
LOCUS       DATMTI010000989         5240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_801555, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000989 DATMTI010000000
VERSION     DATMTI010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801555"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..2264
                     /locus_tag="VN039_15655"
     CDS             21..2264
                     /locus_tag="VN039_15655"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWV47433.1"
                     /translation="MSLELAKKLYAKMPEIFEKARKKFGRPLTLAEKILVSHADNFDA
                     QHWERGKAMLALRPDRVAMQDATAQMAMLQFMQAGKKKAAVPSTIHCDHLIRAEMGSE
                     KDLLRAMDENKEVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGGLIIGTDSHT
                     PNGGGLGMLAIGVGGADAGEVMAGLPWEVLHPKLIGVKLTGKLSGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDQKMVDYLNITD
                     RADLAKLAQANKALLIADPEVNQSPEKYYDQIVEIDLSKLEPHVVGPHTPDLARPVSK
                     VAAEAKEKGYPVELKAALIGSCTNSSYEDISRSAHIARQGLKAGLKAKTSFLVSPGSE
                     RIYHTMKRDGFLDTFEKLGGTVLSNSCGPCIGQWKRADGVKGKADSIVSSFNRNFPGR
                     NDGINETLSFLASPEIVTAYALTGDLRFDPINQNLKAADGKEFKLEPPVGEELPAKGF
                     AKGEEGYVAPAEDGSNLTVDIPPTSERLQLLQPFPRWDGKDFDKLPLLIKTKGKTTTD
                     HISPAGPWLKFRGHLDKISDNMFLGANSAFASEPGKGTNVLTGESNLTIAQIARAYKG
                     KGIGSIVVGDENYGEGSSREHAAMSPRFLNVRAVLTKSFARIHETNLKKQGILPLTFA
                     DPKDYDKIEMNDRLSVTDLANLAPGKPVTVVVHKAGGDVKIQANHSMTTQQITWFKAG
                     SALNALN"
     gene            2288..4516
                     /locus_tag="VN039_15660"
     CDS             2288..4516
                     /locus_tag="VN039_15660"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWV47434.1"
                     /translation="MAKADKIIYTKTDEAPMLATYSFLPIINAFTKAAGVTVELRDIS
                     LAGRVIAVFPEYLTPEQKQHDALAELGEMAKTAEANIIKLPNISASIPQLVATIKELQ
                     KQGYKLPDYPENPKDDKEKDIKARYDKVKGSAVNPVLREGNSDRRAPLSVKAYARKHP
                     HKMGAWSPDSKTHVSHMKGGDFRSNEKSLTIPAATTAKIEFVGADGKITVLKEKIALQ
                     AGEVLDATFMSVKALRKFLEEQIEDAKAKGILFSLHMKATMMKVSDPKIFGHAVTVYY
                     KDVFEKHGETFKKLGVDPDNGLGDVYAKIKSLPDDQRKAIEADIQAVYQKRPPMAMVN
                     SDKGITNLHVPSDIIIDASMPPVIRDSGKMWNPQGQLQDVKCVIPDSSYAPVYHEVVE
                     FCKQKGAFDPRTMGTIPNVGLMAQAAEEYGSHDKTFKAPGNGTIRIVDASGKTLHEHK
                     VEEGDIWRACQVKDAPIQDWVKLAVTRARATGAPAVFWLNKDRAHDAELIKKVNAYLP
                     KHDTTGLEIKIMAPADACRFSIERMKEGKDTISCTGNVLRDYLTDLFPILEIGTSAKM
                     LSIVPLLNGGGLFETGAGGSAPKHVQQFQEEGYLRWDSLGEFLALAASLEHLANVGNN
                     AVAKILADTLDQANAKFLESNKSPARKVGEIDNRGSHFYLALYWAQALAAQTADKKIA
                     EKFQKIAKDLTDHEKTIDQELLAAQGKPQDVGGYYHPDDSKAAKAMRPSATLNRIVDA
                     IA"
     gene            4601..>5240
                     /locus_tag="VN039_15665"
     CDS             4601..>5240
                     /locus_tag="VN039_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWV47435.1"
                     /translation="MSQEDTNVIDQPEVMKPRMVTIEIAGKKVDVPEGITVIKAMWYA
                     GMDVVRGVGCLGGFCGACATYYRTKDDPKVRTCLACQTAVQDGMSFTMMPPFPARKAT
                     YEIQTLQDPKQDLFNFYPEAPLCRNCNACTEACPQSIDVREGVWKAVFGDFKSVSEMF
                     MDCVMCGMCTPVCIADIAPNLVALYVSRAQGAHFSEQPVSLDTRIKEIQDGRF"
BASE COUNT         1236 a         1399 c         1612 g          993 t
ORIGIN      
        1 ctgtttgtaa ggagagcgca atgtcacttg agctcgcgaa gaagctctac gccaaaatgc
       61 cggagatctt cgagaaggcc agaaagaaat tcggccgtcc gttgacgttg gcggaaaaga
      121 ttctcgtttc acatgccgac aacttcgatg cccaacattg ggagcgtggg aaggccatgt
      181 tggccctccg tcccgaccgc gtggcgatgc aagatgccac ggcgcaaatg gccatgttgc
      241 agttcatgca ggccggcaag aagaaagccg ctgtgccgag cacgatccac tgcgaccact
      301 tgatccgtgc cgagatggga tctgaaaaag atctcctgcg cgccatggat gaaaacaagg
      361 aagtttacaa cttcctggct tccgcggcga agaagtacgg catcggcttt tggaagccgg
      421 gtgccggcat cattcaccag gtggtgctgg agaattatgc gtttccgggc ggcttgatca
      481 tcggcaccga ctctcataca ccgaacggcg gcggtctggg tatgttggcc atcggtgtcg
      541 gaggagcgga tgccggagaa gtcatggccg gtctcccgtg ggaggtgctc cacccgaagt
      601 tgatcggcgt gaaactgacc ggcaagttga gcgggtgggc gtctccgaag gatgtgatcc
      661 tctatctttg cggcctgctc accgtaaagg gcgggacgaa caagatcgtc gaatacttcg
      721 gtcccggcgc cgagacgatc agcgcgaccg ggaaaggcac tatctgcaat atgggcgcgg
      781 aactgggggc gaccacctcg gtatttccct tcgatcagaa gatggtcgac tacctgaaca
      841 tcaccgatcg ggcggatttg gcgaaattgg cccaagcaaa caaggcgttg ttgatagccg
      901 atcccgaagt aaatcagtcg cccgaaaagt attatgacca gatcgtcgaa atcgacctat
      961 ccaaacttga accgcatgtg gtcggccccc atactcccga tttggccagg cctgtttcca
     1021 aggttgcggc cgaggcgaag gagaagggct atccggtcga attgaaggcg gcgctgatcg
     1081 ggagctgcac caattcgtcg tatgaagaca tcagccgatc agcgcacatt gcgcggcagg
     1141 ggttgaaggc tgggttgaaa gccaagacgt cgttcctcgt ttcacccggc tccgagcgca
     1201 tctatcatac gatgaaacgg gacggattcc tggacacgtt cgagaagctc ggcggtaccg
     1261 tgctgtcgaa ctcgtgcggg ccctgcatcg ggcagtggaa gcgggccgac ggtgtcaaag
     1321 ggaaggcaga ttcgatcgtc agttccttca accggaactt tccaggccgc aatgacggca
     1381 taaatgaaac actgtcgttt ctggccagtc cggaaatcgt gacggcctat gcgctgacgg
     1441 gcgatttacg gttcgatccc atcaatcaga acctgaaagc cgccgatggt aaggagttta
     1501 agcttgagcc tccggtggga gaggaactgc ccgccaaggg ttttgccaaa ggagaagagg
     1561 ggtatgtggc gccggccgaa gacggttcaa acttgaccgt cgatattcca ccgaccagcg
     1621 aacggctgca attgctgcaa ccgttcccgc gttgggatgg gaaggacttc gataaactcc
     1681 cgctcttgat caagacgaag ggcaagacga cgacggacca catttcaccg gccgggccct
     1741 ggcttaagtt ccgcggccac ttggataaga tcagcgataa catgtttctc ggtgccaaca
     1801 gcgcctttgc atcggagccc ggcaagggca ccaacgtctt gaccggcgaa tcgaacctga
     1861 ccatcgcgca gatcgcccgt gcttacaagg ggaagggcat cgggtcaatc gtcgtcggcg
     1921 acgagaacta tggcgaaggc agcagtcgtg agcatgcggc catgtcgccg aggttcctca
     1981 atgtgcgtgc ggtcctgacg aagagcttcg cacgcatcca tgaaacgaat ttgaaaaaac
     2041 agggtattct cccgctgacg ttcgccgacc cgaaggatta cgacaagatc gagatgaacg
     2101 atcggttgag cgtaacggac ctggcaaatc tggcgccggg caaaccggtg acggtggtcg
     2161 ttcacaaagc ggggggcgat gtgaagatcc aagcgaatca cagcatgaca acacaacaaa
     2221 tcacgtggtt taaggccggt tcggcgctga acgcgctgaa ctaaacgata tattgagagg
     2281 gagaatcatg gcgaaagcag acaaaatcat ctatacaaag accgatgaag cgccgatgct
     2341 ggcgacctat tcgtttctac ccatcatcaa cgcattcacg aaggcggcgg gcgttactgt
     2401 ggagctgcgc gacatttcgc ttgcgggtcg cgtgatcgcc gtgttcccgg aatatttgac
     2461 gccggaacag aagcagcacg atgcgttggc tgagttgggc gagatggcca aaacggcgga
     2521 ggccaacatt atcaagcttc cgaacatcag cgcctccatc ccgcaattgg tcgcgacgat
     2581 caaggaattg cagaagcagg gctacaagct gccggactat ccggaaaatc caaaggatga
     2641 taaggagaaa gacatcaagg cccgctacga caaagtgaag ggcagcgcgg tcaacccggt
     2701 gttgcgcgaa gggaattccg accggcgggc gccgttgtcc gtgaaggcct atgcccggaa
     2761 gcacccgcac aagatgggcg cctggtcgcc ggattcaaaa acccacgtct cccacatgaa
     2821 gggaggcgac ttccgctcga acgagaagtc tctcacgatt cccgccgcga ccacagccaa
     2881 gatcgaattc gtcggtgcag acgggaagat caccgtgctg aaggagaaga tcgcgttgca
     2941 ggccggcgag gtgctggatg ccaccttcat gagcgtgaag gctctgcgca agttcttgga
     3001 agaacaaatc gaggatgcga aggccaaggg tatcctgttc tcgttgcata tgaaggccac
     3061 catgatgaag gtctccgacc cgaagatctt cggccatgcc gtgacggtct actataagga
     3121 tgtattcgaa aaacacggcg agacattcaa gaagctcggt gtcgatccgg acaacggtct
     3181 cggcgacgtc tatgcgaaga tcaagtcctt gccggacgat caacgcaagg cgatcgaagc
     3241 cgacattcag gccgtctatc agaagcggcc gcccatggcg atggtgaata gcgataaggg
     3301 catcaccaat ctccatgtgc cgagcgatat catcatcgat gcctctatgc cgccggtgat
     3361 ccgcgattcc ggcaagatgt ggaacccgca aggccagttg caggacgtga agtgcgtgat
     3421 ccccgactcg agttatgcgc cggtctatca cgaagtcgtc gaattctgta agcagaaggg
     3481 cgccttcgat cctcgtacca tggggacgat tccgaacgtc ggcttgatgg cgcaggcggc
     3541 ggaagaatac ggctcgcacg acaagacatt caaagcgccg ggcaacggca cgatccgcat
     3601 cgtggatgcg tccggcaaga ccttgcacga gcacaaagtc gaagagggtg acatctggcg
     3661 cgcgtgccag gtgaaggatg ctcctattca ggactgggtg aagttggctg ttacccgtgc
     3721 cagagcgact ggtgccccgg cggtcttttg gttgaacaag gatcgcgcgc atgatgccga
     3781 gctgatcaag aaggtcaatg cctatctgcc gaaacacgat acgaccggtc ttgagatcaa
     3841 gatcatggcg cccgcagatg cctgccgctt ttcgatcgag cgaatgaagg aaggcaaaga
     3901 cacgatttcc tgcaccggta acgtgctccg tgattatctc accgacctct tcccgattct
     3961 cgaaatcggc accagcgcca agatgctctc gatcgtcccg ttgttgaacg gcggggggtt
     4021 gttcgagacc ggtgccggcg gatcggcccc caagcatgtg cagcaattcc aggaagaggg
     4081 atatcttcgc tgggattcgc tcggcgaatt cttggcgttg gctgcttcgc tggagcacct
     4141 ggcaaatgtg ggaaacaatg cggtggccaa gattctggcg gatacgctgg atcaagccaa
     4201 tgcgaagttt ctggagagca acaagtcacc ggctcgtaag gtcggcgaga tcgacaaccg
     4261 cggcagccac ttctacttgg cgctctattg ggcgcaagcc ttggccgcgc agacggcgga
     4321 caagaagatc gcggagaagt tccagaagat cgccaaggac ctgaccgacc acgagaagac
     4381 gatcgatcag gagttgttgg cggcgcaggg gaagccgcag gacgtcggcg ggtactatca
     4441 tccggacgac tcgaaggctg cgaaggcgat gcgcccaagc gcgacgttga atcggatcgt
     4501 cgacgcgatc gcataggatt gcgttagggg ttaggcgtca ggcgtgaggg gattgctttt
     4561 caccccttac gcctcacgtt tcacgcctca cgagggaaca atgagccaag aagataccaa
     4621 cgtgatcgat cagcccgagg tgatgaagcc ccggatggtg acgatcgaga tcgccggcaa
     4681 gaaagtggac gtgccggaag ggatcacggt catcaaggcc atgtggtatg cgggcatgga
     4741 tgtcgttcgt ggcgtcggat gcctcggagg tttctgcggt gcctgcgcga cctactatcg
     4801 gacgaaggac gatccgaaag tccgcacctg tttggcctgt cagactgcgg tgcaggacgg
     4861 catgtcgttt acgatgatgc cgccgtttcc ggcgcgcaag gcaacctatg agatccagac
     4921 actacaagat ccgaagcaag acctgttcaa tttctacccg gaagcgccgc tctgcaggaa
     4981 ttgcaatgcc tgcaccgagg cctgtccgca aagtatcgac gtgcgagagg gcgtctggaa
     5041 agccgtcttc ggtgacttca aaagtgtctc cgagatgttc atggattgcg tcatgtgcgg
     5101 catgtgcacg ccggtctgca tcgcggacat cgcgccgaat ctcgtggcac tgtacgtgag
     5161 ccgggcgcag ggggcgcatt tttccgaaca gccggtgagc ctcgatacgc gcattaagga
     5221 gattcaagac ggtcggttca
//
LOCUS       DATMTI010000990         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_801574, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000990 DATMTI010000000
VERSION     DATMTI010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801574"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..190
                     /locus_tag="VN039_15670"
     tRNA            113..190
                     /locus_tag="VN039_15670"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Asp,seq:gtc)
     gene            265..966
                     /locus_tag="VN039_15675"
     CDS             265..966
                     /locus_tag="VN039_15675"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWV47436.1"
                     /translation="MFQTGPLGVVLSLGIVSKVVLLLLVCFSITSWAIIFYKLAVFRT
                     ADAKDRHFMTVLLRARDVEEVTRHAQQTVGSPCAKIFHAVIQRIGYIPTEVNENGSVA
                     YDRHVMERTAAHLAQGQIAKLESFLPFLATTGNICPFVGLLGTVMGIIDSFREIGTQG
                     TASIAAVAPGVSEALIATAAGLFAAIPAVVAYNYFLIRIRRAAMHMDSVVVELLALIP
                     AQTRPVAPVPVGAKG"
     gene            963..1406
                     /locus_tag="VN039_15680"
     CDS             963..1406
                     /locus_tag="VN039_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWV47437.1"
                     /translation="MMFETRQRRFLAEINVIPLVDVVLVLLVIFMVTAPMLYRGMDIK
                     LPTSASNTIKPEIRAVLTIEKDQRLYLDKDQVSVAQLERKLRVMKEEHADVSLYLRAD
                     RDVPYGIVVQVMDGVKKAGIEKLGMVTDPTGPEHVSEGTPSQHNQ"
BASE COUNT          331 a          401 c          460 g          388 t
ORIGIN      
        1 agaacaggaa gtgtccttgc cgcgttgagg atctgttcct acacgcagga attgatcggc
       61 gaccaacgat ttcatcttgt cgtgctgcta ttctgccaga gatagatttt gcggggccgt
      121 cgttcagcct ggttaggacg ccagattgtc aatctggagg tcgcgggttc aaatcccgtc
      181 ggccccgcca ttttcttcag agtttggtac aattccactt tgtcaaggaa tcatgaacgc
      241 tgaggaggga gagtagagat tcgtatgttt caaaccggcc cactaggagt cgttctgtcg
      301 ctcgggatcg tgtccaaggt ggtcctcttg ctcctggtct gcttttcgat cacgtcatgg
      361 gccatcatct tctacaaatt ggccgtattt cgaaccgctg atgcaaaaga tcgtcatttt
      421 atgaccgtct tgttgcgggc cagggatgtg gaggaagtca ctcggcatgc gcaacagacg
      481 gttgggagtc cctgcgcaaa gatttttcat gctgtgattc aacggatcgg ctacattccc
      541 accgaggtga atgaaaacgg ttccgtcgcg tacgatcggc atgtgatgga acgcacagca
      601 gcccatcttg ctcaggggca aatcgccaag ttggaatcgt ttcttccgtt tcttgcgacg
      661 acaggaaata tctgtccatt cgtgggcctc ttgggaacgg tgatgggtat tatcgactca
      721 ttccgggaaa tcggcacaca aggcacagcc agcattgcgg cggtggctcc cggcgtctcc
      781 gaagccttga ttgcgactgc ggccggattg tttgccgcga ttcccgccgt cgtcgcctat
      841 aattattttc tcatacgtat cagacgcgcg gccatgcata tggattcggt cgtggtggag
      901 ctcctggcct tgattcccgc ccaaacgaga cctgtcgctc cggttcccgt gggagcgaag
      961 ggatgatgtt tgagacgaga cagcgccgat ttttggcgga gatcaatgtg attccgctcg
     1021 tggacgttgt gctggtgctt ctagtgatct ttatggtcac ggcaccgatg ctctatcgcg
     1081 gcatggacat caagttgccg acttcagcat cgaacacgat caagccggag atccgagcgg
     1141 tgctgacgat cgaaaaggat caacgtctgt acctcgacaa agatcaggtg agcgtggctc
     1201 agttggagcg gaaattgcgc gtgatgaaag aagaacatgc cgatgtctca ttgtatttac
     1261 gcgccgaccg cgacgtgcca tatggaattg tggttcaggt gatggatgga gtcaaaaagg
     1321 ccggtatcga aaagctcggc atggtgactg atcccaccgg acccgaacat gtcagtgagg
     1381 gtacgccatc ccaacacaac cagtaggata aggtccacag tgcaggcttg ggcatcggcc
     1441 ggcatggtct cgggtgatga acggcaggcg acactgactc gtcgcttagg ctttgccgtt
     1501 gtcgtctccc tggtactcca tatcggcata ttggcgatgg tggcctgggt tcgactgccg
     1561 cgacatggcg aacgtcccct
//
LOCUS       DATMTI010000991         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_801604, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000991 DATMTI010000000
VERSION     DATMTI010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801604"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..894
                     /locus_tag="VN039_15685"
     CDS             235..894
                     /locus_tag="VN039_15685"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWV47438.1"
                     /translation="MSDPQLTVDTLKTSLREAQRMVAELRAEVDARRQELADVQIARA
                     QLEGRIRETERRLIEARHVINLQREELSNARSEREQVGRTRSALRNQLKQLQKQLSKA
                     EKQVMGNVSPAAMAPPRDGQHELATIDPQQDAKLATLEVSGAPLFDEAPVVSQSTTVP
                     SHLHVLVKSGDTLWSIARRYHTSVRRLRALNALPSDRILVGQAIWLTEPAVEMSEHER
                     M"
     gene            939..>2128
                     /locus_tag="VN039_15690"
     CDS             939..>2128
                     /locus_tag="VN039_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWV47439.1"
                     /translation="MAVFTYRVARPDGSTIHGHVEGENESLARAKLESQGLLVFSLHR
                     RGITSVKTEMSWSWGKLPLGQFLVFNQELLALVKSGLPILRIWDLLIERAGHGGFQRT
                     LRDVREDIRGGASASDALAKHPIYFPELYVATVKAGEQSGNLPEVLQRYVAYLKLMVG
                     LRQKVKKAISYPIVLIGIGMAVVGFLLTYVMPTFVSVYGESAKTLPWATQVLLDMVMH
                     AESRIVPAVVVVIGIMLGIHAYYATPTGQLAIDRLVLKLPLVGQIAVEHNTVQLTRTL
                     GTILAGGTPLVDALQGTRGAVSNRWISQETIGAVNEIREGATLADALDRFRVLPRLAI
                     EMLSVGEETGSLDAMLRDVAEFYESDLDTKLTQLTTWIEPVLLLVMGVLVGGIVIVMY
                     LPVF"
BASE COUNT          486 a          517 c          622 g          503 t
ORIGIN      
        1 tgaaccgaca gagggtggta ttttcgatgt gtattccgga tctgaccttg ttgggacaaa
       61 cgggactcca tataaccaat ggtgaatagg catgtgagat tggctctgag tagattctgc
      121 cctaggctca atgtgttacg agtgcggggg gaatggggaa gtacatcaat tggaatcctt
      181 ctgctcggaa tgtcggcctg tagtccactt gagcccatca tcatagagcc ggagatgtcg
      241 gatccccaac tcacggtgga tactctcaag acctcattgc gagaggctca gcgaatggtt
      301 gccgagttga gagcagaggt cgacgcacga cggcaggaat tggccgatgt gcagatcgca
      361 cgggcgcagc ttgaaggacg tattcgggag acggaacgtc ggctgatcga agctcgccat
      421 gtgattaatc tccagcggga agaactaagc aatgctcgtt ccgaacggga acaggtgggg
      481 cggaccagat ccgcactgcg gaatcaactg aagcagcttc agaagcagtt atcgaaagca
      541 gagaagcagg tgatgggaaa tgtctctccc gcggctatgg ctcctccacg agatgggcaa
      601 catgagttgg cgactattga ccctcaacag gatgccaagt tggccactct ggaggtgtcc
      661 ggagcacctc tgttcgacga agcaccggtg gtttcacagt cgacaactgt gccctcgcac
      721 ttacacgtgt tggtcaagtc tggggacacg ttgtggagta tcgcacgccg atatcacact
      781 tccgtcagac gccttagggc tctcaacgcg cttcctagtg accgtattct tgttggtcaa
      841 gccatctggc ttactgagcc agctgttgaa atgtccgagc atgaacgaat gtagtgacgc
      901 acgatgggct aagctgcaag tcgtgtaggt ctacatctat ggcagtattt acgtatcgag
      961 tcgcacggcc tgatgggtcc acaatccatg gccatgtcga aggagaaaat gaatcgttgg
     1021 ctcgtgccaa actagagtcc caaggattgc tggtttttag tcttcaccgt cgaggaatca
     1081 cctcagtcaa gacggagatg tcgtggtcat ggggaaagct tccgctgggg cagttcttgg
     1141 tcttcaatca agaattgctg gctctggtca agtccggact gcctattttg cgcatctggg
     1201 atctcctgat cgaacgagcc ggccatggtg gatttcaacg gacactacgt gacgtgcggg
     1261 aagacatccg aggcggagcc tcggcctcgg atgcattagc taagcacccc atatatttcc
     1321 cggaactcta tgttgccacg gtaaaggcag gagagcaatc gggcaatctt ccggaggtgc
     1381 tccagcggta cgttgcatat ctgaagctga tggtcgggct tcgtcagaaa gtgaagaagg
     1441 caatctccta tccgattgtt ctcatcggta tcggcatggc cgtggtcggg tttctactga
     1501 cctatgtcat gccgactttc gtatcggtct atggagagtc agcgaagacc ttgccatggg
     1561 cgactcaagt tttgctcgat atggtcatgc atgccgagtc acggatagtg cctgcagtag
     1621 tcgtcgtgat cggcattatg ctagggatac atgcctacta tgccaccccg acaggacagc
     1681 tggcgatcga tcgtcttgtg ctaaagctac cactcgtagg tcagattgca gtcgaacaca
     1741 ataccgtcca gctgacgcga acgctcggaa ctattcttgc cggaggcacc cccctcgttg
     1801 atgccttgca aggtacacga ggggccgttt ctaatcgatg gatttcacaa gaaacgatcg
     1861 gggccgtcaa tgagattcgt gagggcgcga cactggctga tgcgttggat cgtttcagag
     1921 tgctcccgag gctcgcaatc gaaatgttat cggtaggaga ggaaacgggt tcgctcgatg
     1981 cgatgttgcg ggacgtcgcg gagttttatg aatctgatct cgatacgaag cttacgcaac
     2041 tcaccacatg gatagagccg gttctgctgc ttgtcatggg agtgttggtc ggcggaatcg
     2101 tcattgtgat gtacctacca gtatttca
//
LOCUS       DATMTI010000992         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_250730, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000992 DATMTI010000000
VERSION     DATMTI010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250730"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VN039_15695"
     CDS             complement(<1..667)
                     /locus_tag="VN039_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47440.1"
                     /translation="MTKWQAGVLVTCVFILMTSLPSAWATSNDTPADDGPSLEETLVF
                     IKDTWEACATMHSGLQRMIAKDGSLWHMDRMAKVDVVVMPPSALKIITRLNELRKLSG
                     IFGIQSPVEKIQEFDLNALSPNVSPEREGENITLYGIRLRCARGACVTEWLASLAIPG
                     KPMHELKTGKVVVTSLEELVYEDQGPKDKPGEKKGEVYIPVCDIASLESLSKAFHHAI
                     IKVG"
     gene            complement(870..946)
                     /locus_tag="VN039_15700"
     tRNA            complement(870..946)
                     /locus_tag="VN039_15700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(910..912),aa:Arg,seq:tcg)
     gene            complement(1012..1512)
                     /locus_tag="VN039_15705"
     CDS             complement(1012..1512)
                     /locus_tag="VN039_15705"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWV47441.1"
                     /translation="MNVVLIGYRGTGKSTVGKILAARLGHILVSTDAEIVKLVGQSIP
                     EIVEQNGWEHFRDLEAKVCQALAGRTGLVVDTGGGAILRSRNVEVLKATGRLFWLTAS
                     VETIAERIGRDSQRPSLTGVKSFVDEIQDVLRERTPKYQAAADYVVETDGRSVTQVAE
                     EILARL"
     gene            complement(1509..2363)
                     /locus_tag="VN039_15710"
     CDS             complement(1509..2363)
                     /locus_tag="VN039_15710"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWV47442.1"
                     /translation="MDIDVRTQFCGVIGNPVGHSLSPAIHNAAFRKLGLNFVYLAWQV
                     ETIGDAIKGLRALGNFRGASVTIPHKVAAIPFLDHVERTAQRIGAINTIVAEQGALTG
                     YNTDATGALRALREGGAELKGRCIVVLGSGGAARAIAFALAVESSADKLTLLGVDDSE
                     RASLAQDIRTQASLTVEDFHLDETTLRRVLPDAHVLIHCTPVGMSPNADTTCIPASLL
                     HNDLSVMDIVYNPLETRLLKDAKRAGCRAIPGLEMFLNQAVTQFEFWTNHPAPVDVMR
                     SVLESHFR"
     gene            complement(2407..3168)
                     /locus_tag="VN039_15715"
     CDS             complement(2407..3168)
                     /locus_tag="VN039_15715"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV47443.1"
                     /translation="MNRLEGKVVVVTGGNAGIGEAIAKRFVEEGASVVITGRRQQELD
                     RVTSVIRLNKGKVLGIAGSVTDETHVQDVARRTLDSFGRIDVLVNNAGIGEFGKRLHE
                     TEDAIWAQVFEINVTGVFRMTRAVIPHMLRQGRGSIINISSIASLVGLSGSAAYAASK
                     GALDALTRALAVEYAKDGIRCNVVNPGLIDTPMAAPLMANPDMLQPILAQYAIRRPGT
                     PEEVANMVLYLASEEAAWVTGATFPIDGGMTVCKG"
     gene            complement(3230..4048)
                     /locus_tag="VN039_15720"
                     /pseudo
     CDS             complement(3230..4048)
                     /locus_tag="VN039_15720"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
BASE COUNT          943 a         1211 c         1019 g          929 t
ORIGIN      
        1 cgccaacttt gatgatggca tggtgaaaag ctttagaaag actttccaag gatgctatgt
       61 cacagacggg aatatagacc tcccccttct tttctccagg tttgtctttt gggccttgat
      121 cctcatacac aagctcctca aggctcgtga cgacgacttt gccggttttt agttcgtgca
      181 tgggtttacc cggtatggct agagatgcaa gccattcagt cacacatgcc ccacgcgcac
      241 agcgcagccg aattccatag agggtgatgt tttcaccctc ccgctcaggg ctcacgttag
      301 gagatagtgc gttcaagtca aactcctgaa tcttttccac cggcgattga attccgaata
      361 tcccgctcag cttcctcaat tcattcagac gtgtgataat tttgagagct gagggaggca
      421 taacaaccac gtcgaccttc gccattcgat ccatatgcca gagagatccg tctttggcaa
      481 tcatccgttg caacccactg tgcattgttg cacatgcttc ccacgtgtcc ttgataaaga
      541 ccaacgtctc ctccagcgac ggcccgtcat cggcgggcgt gtcgttcgat gtcgcccatg
      601 cactcggcag actcgtcatg aggatgaaga cgcacgtgac cagaacccct gcttgccact
      661 tcgtcattat ttttccccca taaaaacata tgccgcgata tgcactcgat cacgatagcg
      721 gtagaattgc tcgagcatta atccctggtg gtaatcggca gacggcagat cagctcaagg
      781 ctgagtctat ccacgccaga gaggatctgc actcgccgca caactgaaag gcaagtcgac
      841 ttggggaatc cggtaactcg ttgaatctat ggtgcgcccg gcaggaattg aacctgcgac
      901 ctacgggttc gaagcccggc gctctatcca actgagctac gggcgcagcg gaacctacat
      961 gaatcatgtt caagcgaggt ttgtcaaggc tgacagaggc ggttgaggca gctacaatcg
     1021 tgccagaatc tcttcggcaa cttgcgtgac ggaccttcca tcagtctcaa cgacgtaatc
     1081 ggcagccgct tgatacttcg gtgtgcgttc acgaaggaca tcttgaatct catccacgaa
     1141 tgacttgact cctgtaaggg aagggcgttg tgaatcccga ccgatacgtt cggctatcgt
     1201 ctcaaccgag gcggtcagcc agaacaacct tcctgttgcc tttaatacct cgacatttcg
     1261 tgatcgaagg atggctcccc cgcccgtatc gacgactaac ccggtgcgac cggccagtgc
     1321 ttggcagacc ttggcttcga gatcacggaa atgctcccac ccattttgct cgacgatctc
     1381 cggaatgctt tgaccgacta gcttcacgat ctccgcatca gtcgacacga gaatgtgacc
     1441 aagtcgagcc gccagtattt tgccgacggt actcttgccg gtcccacgat agccgatcag
     1501 caccacattc atcggaagtg ggattccaag accgaacgca tcacatcaac cggcgcagga
     1561 tgattcgtcc agaactcaaa ttgagtgacg gcttgattga gaaacatctc caacccggga
     1621 atcgccctac accctgcacg cttcgcatct ttgagcaatc tggtttcgag tgggttgtag
     1681 acgatatcca tgaccgagag gtcgttgtga aggagcgaag caggaataca ggtggtatca
     1741 gcgttcggag acataccgac cggggtacaa tgaattagca cgtgtgcgtc ggggagaacc
     1801 cgtcgaagag tggtttcatc aagatgaaag tcttcaaccg tcaatgatgc ctgggtacga
     1861 atatcctgag ctaaactggc ccgttcagaa tcgtcaactc ccagtaacgt caacttgtcc
     1921 gcactggatt caactgccag ggcaaaggcg atcgctcgag cggcgcctcc agagccaagc
     1981 acgacgatgc atcgaccctt gagctcagct ccaccctctc tcaacgccct cagagcacca
     2041 gtcgcgtccg tgttgtaccc agtgagcgca ccctgttcag caacgatggt gttgatcgct
     2101 ccgatccgtt gagccgtcct ttccacatga tcaagaaacg gtatggcagc caccttgtga
     2161 ggaatcgtca cactcgctcc ccgaaagttt cccaacgcgc ggagcccttt gatggcatca
     2221 ccgatcgttt ccacttgcca ggcgagatag acaaagttga gccccaattt gcgaaacgca
     2281 gcattgtgaa tggcaggtga cagtgaatgg ccaaccggat tgccgatcac cccgcaaaac
     2341 tgtgttcgaa catcaatgtc cattcttcac ctaacggaag ttgttactgt tcgtgggcag
     2401 caaccattac cctttacaaa cagtcatgcc cccgtcaata gggaaggtcg ccccagtaac
     2461 ccaggcggct tcctctgagg ccagatacag aaccatattc gccacttcct ccggcgtccc
     2521 aggacgacga atcgcatatt gggcaagaat gggctgaagc atatcgggat tggccatcag
     2581 gggagccgcc ataggcgtat cgatcaggcc aggattcacc acattgcagc ggatcccgtc
     2641 cttcgcatat tcgaccgcca gcgcacgtgt caaggcgtca agcgcaccct tggatgccgc
     2701 ataggcggct gagccggaga ggcctactaa actggcgatt gacgagatat taatgatcga
     2761 gcctcggcct tgcctcagca tgtgggggat gacagcccgt gtcatgcgga ataccccggt
     2821 cacgttgatt tcgaacactt gcgcccagat cgcatcctcc gtttcgtgga gccgttttcc
     2881 aaattcaccg attcccgcat tgttgaccag aacgtcgatc cgcccaaaac tatcaagggt
     2941 tcgacgggcg acatcctgca catgagtttc atccgtcact gaaccggcaa ttccgagtac
     3001 ctttcctttg ttgagtcgga ttacactggt tacacgatcc aattcctgtt gccgtcgccc
     3061 cgtgatgaca accgatgctc cctcctcgac gaaacgtttc gcgatcgctt caccgatgcc
     3121 ggcattgcct cccgtcacca caactacctt gccttccaac ctattcataa gttcggttcc
     3181 tcttgtgctt catcctgtgt gccttcctgg tccctatcgg caggagctcc tatgtcagca
     3241 ttggcccaag gagcccgggc tcgaccttac gagccactgt gatgaaccca gaatgagcca
     3301 ccatgcgatg atcaggccgc acgctcctgc cttgcacgga ccaggtcctc agcaacgtct
     3361 caaacgtttc gatcattcca aatacagctg tgcgctcaag cgcctctacc gtttgcatga
     3421 cttgcggtac tgtggggacg aagctcagat agatcccgcc tgatcgaagt gctttcatgg
     3481 catgcggtac gacctgccat ggttccggca aatcaagcac cacgcgatcg aaaggcacct
     3541 ggtcgtccag ttaatcgatg ccctcatagg catttttcct gaaacagatc agattgattg
     3601 gattcagaga acgggcaatg tttgtctttg ccgtccgtgc aaaatcttcc cggatctcat
     3661 acgtcaccac caatccaccc ggccccactg cgcgcaatag agccatggtc aaggctcctg
     3721 aaccggtccc ggcctcgaag actcgagcac ccgggtagat atcggcccac atgggtatga
     3781 tcgcgagatc cttaggatac aaaacctgcg cccctcgggg catcttgagc acatagtcgc
     3841 cgaaggtcgg ccggagcgcc accatcttct taccaccgga cagtgtgacg atggacccgt
     3901 cgagacggcc gatcaacgca tcatgtgcga ttttctgccc actgaactga tacgtctccc
     3961 ccgctttcaa cgtgagagca tactgtcgcc gtttttgatc gacaaggtgg atgcgttcgc
     4021 cattgaaaaa gcgagacatg ccgcgcattc taggaggttc gttccatggt ttgcaaccaa
     4081 gcgcccttcc ttgacagggt ag
//
LOCUS       DATMTI010000993         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_377402, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000993 DATMTI010000000
VERSION     DATMTI010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377402"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..2075)
                     /locus_tag="VN039_15725"
     CDS             complement(135..2075)
                     /locus_tag="VN039_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV47444.1"
                     /translation="MSDKRPLFQGSLAVAEASRDRTPYSGFLAGLFEGAVRRDLFRSQ
                     SIPPLRDTAKEFLEHLRLLLIEKVDPESIDREGEVGEDVLAALKDIGAFGLKIPSIYG
                     GLGLTQSEYHRVATLLGSHDAATTVLISAHNSIGAAEPVKLIGNREQQQRLLPRLARG
                     DISGFALTEKGAGCDIWDLRSYAIPVREDGVLVGYRLTGDKLYTTNAPRRHNEFLASL
                     LVVIAQIVHHPDEVTRPKSERRFGAFVVDTRTPGCHCTRLHYMGVRGIYNGAVRFDNV
                     FVQVANRLGDEGEGLRRALESLTVGRLTLPAACLGNLKQCLWLARLRAQQRIQYDRPI
                     GEHTDIGSKIVRMASRTLALEALVTITGIWADAKIDVRLESAAVKILATEWLLESVLD
                     LFRIYGGRGFETSESLRLHGDLPVPIERMVRDALINVIWEGTNGILTLWIGREGLAEY
                     VSQGNAFLEFQIGEMAQALPFFAKVAGRSFKRLPRIGGWPASASPESAWERFVTKKSR
                     ELARKALWVVARHRQGLARKQLLMTHLVKAGMQLFAVESLVWYASQRQASWNRQLADG
                     LVQHFCSQIEDQFNPRPLLSLRRSLWQDDSHVFRLAQDILAGKAEWLEEGILAWHPAS
                     HGRDDQLAFTMGGESAEFPTTY"
     gene            complement(2088..>3097)
                     /locus_tag="VN039_15730"
     CDS             complement(2088..>3097)
                     /locus_tag="VN039_15730"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWV47445.1"
                     /translation="NLSGTRPYYFEHRDIFRPAIGEGVKTIWRATKQLIPLVRLYARN
                     YQEMTFIDLLDKAQDALPSGIYRLEPFARYLEKTFMAKGLGLRFTDLRKDLYIPAIDL
                     ESGECVIFGEEGRRDISIAQAVTASSAAPIYFCPVRIDGRDYVDAGIGRPAFFDLAIS
                     KHVDLMVMINPMGRIPLPNSSWAESALTKRHGRLRDKGFLTIGEQASRINLDARMSQA
                     LTMFRQDCPDKELMMVTPGPHDALLFERSFLCYRDRVQLLRAGYLSVATLVGEAYENI
                     HAQFARHGITLERTRFEDRAASRMAQLDQIVTVSTPLPRRGKDVAMGLDPSRAALHQA
                     K"
BASE COUNT          663 a          915 c          878 g          641 t
ORIGIN      
        1 atcgctcctc aatctatagc ggtccttgac caattcgaag tattccgcaa tttccgtcat
       61 gccgacctcg tcatggggtg atcctcacgt ctaggagcgg tctggatgaa acgactcact
      121 ccttcttacg tcgctcagta cgtcgtggga aattccgccg actctcctcc catcgtgaac
      181 gcgagctgat cgtcccggcc gtgcgaggcc gggtgccaag caaggatccc ttcctccagc
      241 cactcggcct tgccggcaag aatatcctgc gccaaacgga agacatgcga gtcgtcctgc
      301 cacaacgatc gtctgagaga gagcaagggt ctgggattga actggtcttc gatctgtgaa
      361 caaaaatgct gtacgagccc atcggccaac tgacgattcc aggaggcctg ccgttgcgag
      421 gcgtaccata ccagagattc caccgcaaag agttgcattc cggctttgac gaggtgtgtc
      481 atcaagagtt gcttacgcgc gaggccttgg cgatgacgag ctacgaccca tagcgccttc
      541 cgtgcgagtt cgcgggactt cttcgtgacg aaccgctccc atgccgattc aggggaggca
      601 gaagccggcc atcctccaat ccgaggcaag cgcttgaagg aacggcccgc gactttggca
      661 aagaacggca gagcctgtgc catctcgccg atctggaatt ctaagaaagc gttaccttgg
      721 gatacgtact cagccaatcc ttcacgtccg atccagaggg tcaagatgcc gttcgtccct
      781 tcccaaatca cattgatcag cgcatcgcgg accatgcgct cgatcggtac cggcagatcc
      841 ccatggagcc ggagcgattc cgacgtttcg aacccgcgcc cgccataaat gcggaacaga
      901 tcgagcacag attcgagcag ccattcggtc gcgaggattt tgacggcggc cgactcgagc
      961 cgcacatcga tcttggcatc ggcccatatc cctgtgatcg tgaccaacgc ctccagtgcg
     1021 agggtgcgtg aagccatgcg cacgattttc gatccgatgt ccgtgtgctc gccgatcggt
     1081 cgatcatatt ggatgcgctg ctgtgcgcga agccgagcga gccagaggca ttgtttcaga
     1141 ttcccgagac aggccgcggg aagggtcagg cggcccacgg tcaaactttc caaggcccgc
     1201 cgtaaccctt ctccctcatc ccctaaacgg ttagccactt ggacgaagac gttgtcaaac
     1261 cggacggcgc cgttgtagat gccgcgtacg cccatataat ggagcctggt gcagtggcac
     1321 cccggtgtgc gggtgtcgac gacaaacgct ccgaaccgcc gttccgattt tgggcgagtg
     1381 acctcgtcag gatggtgtac gatttgagca atcaccacca ggagcgacgc caggaactca
     1441 ttgtgtcgtc tgggcgcgtt cgtcgtgtac agtttgtcac cggtcaatcg atagcccact
     1501 agaacaccgt cctcgcgtac cgggatggca tagcttcgaa ggtcccaaat atcgcagccg
     1561 gcacctttct ctgtaagcgc aaacccggaa atgtcgccac gcgcaaggcg cggcaacaat
     1621 cgttgttgct gctcgcggtt cccgatgagc ttgactggtt ccgctgctcc gatcgagttg
     1681 tgcgccgaaa tcagcaccgt ggtcgcggcg tcgtggcttc cgagaagtgt cgccacgcgg
     1741 tggtactccg actgagtcaa gccgagtcct ccatagatgg atggaatttt caagccgaag
     1801 gcaccgatgt ccttgagcgc cgcgaggaca tcctcgccga cttccccctc tcgatcgatg
     1861 ctctccggat caactttctc gatcagaagg agacgtagat gttcaagaaa ctccttcgct
     1921 gtgtcgcgga gcggcgggat cgactgtgat cggaagagat cgcggcgaac cgctccctcg
     1981 aacaggccgg ccagaaagcc cgaataggga gtccgatccc gcgaggcttc cgctacggcg
     2041 agggatcctt gaaagagagg cctcttgtcc gacattgtca tcccatccta ctttgcttgg
     2101 tgtaaggcgg cacgagaggg gtctaagccc atcgccacat ctttaccccg gcgcggaaga
     2161 ggagtggaga ccgtcacaat ctgatcaagt tgcgccatgc gcgaggcagc acggtcctca
     2221 aatctggttc gttcgagcgt gatcccatgg cgtgcgaact gtgcatgaat gttctcgtat
     2281 gcttcgccta ccaatgtcgc caccgacaga tatccggctc gaaggagctg gactcgatct
     2341 cgatagcaca aaaaactccg ctcaaacagg agcgcatcgt gggggccagg tgtaaccatc
     2401 atcagttctt tatccggaca gtcctgtcgg aacatcgtca atgcctgaga catgcgagcg
     2461 tcaagattga tccggctggc ctgctcaccg atggtcaaga accccttgtc tcgaagacga
     2521 ccgtggcgct tcgtgagagc cgactctgcc catgacgaat tggggagtgg aatgcgaccc
     2581 atcggattga tcatcaccat gaggtccacg tgtttcgaga tggctaagtc gaagaacgcg
     2641 ggtcgcccga tccctgcgtc gacataatcg cgtccgtcga tgcgcaccgg acagaaatag
     2701 atcggcgcgg cggaggaggc agtcacggcc tgggcgatgg aaatgtcccg ccggccttcc
     2761 tcaccgaaga tcacgcattc cccggattcc aaatcgatgg ccgggatgta caggtctttt
     2821 cgaaggtcgg taaaccgcag ccccaaaccc ttggccatga acgttttctc gaggtatcgg
     2881 gcgaagggct ccaatcggta aatgccgctg ggcagtgcat cttgagcctt gtccagcaga
     2941 tcgatgaacg tcatctcctg atagttgcgt gcatacagtc gaaccaaggg gatcagttgt
     3001 ttcgttgcgc gccagattgt cttcacacct tcgccgattg caggtctgaa aatatcccga
     3061 tgctcgaagt agtacggacg tgttccggac aaattcg
//
LOCUS       DATMTI010000994         4167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_207992, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000994 DATMTI010000000
VERSION     DATMTI010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207992"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..823)
                     /locus_tag="VN039_15735"
     CDS             complement(161..823)
                     /locus_tag="VN039_15735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWV47446.1"
                     /translation="MGAALPLLPIGTTFCYYWLPSALREELLVQFAPQLLAYLAMGLW
                     ASRTVQVPVQLGLEKAKAGNGLKWGMITGLLLGCLNTFVILSVYPSLGYDITFLKQTL
                     HGRLPVLLMVPWFICGIALFVEVNFRGFLLGRLAELESTWCGTHSHRRFSPLALITSA
                     LTFTFDPFMENTFRHLHWIALWDGLIWGAIWLRTRNLWITIVAHAVEVIVMYSAVRAA
                     IG"
     gene            complement(876..2438)
                     /locus_tag="VN039_15740"
     CDS             complement(876..2438)
                     /locus_tag="VN039_15740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV47447.1"
                     /translation="MNRSSPTYHRLATIVIAGLWTILSPPVSWGLDVTQRISSERRET
                     VSLADAAVRALQNNLDISISRHNKESRLADIIIEYSKFDPNVSLNGQYARTVNPLNRP
                     VFGGTGGSLNQITAFDQRSESFTFDAQTNLITGGNFDMNYSPARTNVNANVARGFLFN
                     PSWTGGLAFTLTQPLLKNAGIEINRTFIKVAQNNAEVEQHVFRDRVLTVITTVEQTYW
                     ELVFANENLKVAQAAMKAAEELLASNRAKAKAGVMSIVDVLQAEAAVASRVEQTLVAE
                     KAIRDQEDQLRRLLNPGEEDLRQDVRLTPADPPVTVLEPLSLQEAIDTAIEQRPEIVQ
                     AKKNVESGELNKQFARNQLLPTLSFQGTIGLAGLGSDYGQAFSRNFSGDFYNYGAGLV
                     LSYPLGNRAAINTYNKRKLDVRNAEASLASVRQQIIVGVREAVRRVQTDFKRIETTRS
                     ARIMAEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRAILDYNKSLSNLARHK
                     ATTLDRYHLELS"
     gene            2508..2975
                     /locus_tag="VN039_15745"
     CDS             2508..2975
                     /locus_tag="VN039_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47448.1"
                     /translation="MNITMGGYLIDKAVTDSRRVSIVAVLLQTLAFAALSTSPCAFAQ
                     NASAASGASSVRSFAGGVAPLFTFPGGSLYIDNQGTQGFLHTPGQNFESFSFRNPTTG
                     QAWSGAAMTFGPQLSIGLIQGANQVGNSTVLPGPPPQTSPLPPIESGILDEIP"
     gene            complement(3071..>4167)
                     /locus_tag="VN039_15750"
     CDS             complement(3071..>4167)
                     /locus_tag="VN039_15750"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWV47449.1"
                     /translation="VFDSPSADLQAQIVFVVYGIVDPAQGIDDYAGVDVNDCIVLFLR
                     GKAEHYPRAVSHADKVRFARERGAIGYLTATGPILHPYEVRRGIHGSPSAFYGQLPLD
                     QAIPGAWVSTALAKELLGGSSTDMPDRLRVLQERLNQASSSQATRTDRFASLQWKTTT
                     AEGILMNVIGMIPGTGTESVIIGAHRDHFGQPGGMLFPGADDNASGTAVILEVARALS
                     KVEPRPSRTILFVSFSGEERDLLGSRLYTSRPVVPLNSTKAMINIDHAGVGNGRLTVG
                     VTVVEKEGLLEVGKTAGVHEKLDLYGFFPGGDHVPFKEAGVPTVTVVSGGVHPHFHQP
                     SDTAETIDPEILKTVTRYVLAVAWQLAYQP"
BASE COUNT          896 a         1150 c         1171 g          950 t
ORIGIN      
        1 ggtcccaatc catcgttgtg cccgaggtcg aagagcacgg cccgtttcga ccagcggatg
       61 tccacgtaga caccgggatc accaaataca tcgttgatca gataagtgga gaaggatggg
      121 ttcatagaat tatcctctgc cgccgacaac gaatcacaga ttatcctatc gcagccctta
      181 cggcactata catcacgatg acttcaaccg cgtgagcgac gatggtaatc caaaggtttc
      241 gcgttcggag ccagatagct ccccaaatga gtccatccca caaagcaatc cagtggagat
      301 ggcgaaacgt attctccatg aacggatcga aagtaaacgt gagcgcgctc gtgatcaacg
      361 caagaggaga aaagcgtcgg tgcgaatgtg ttccacacca ggtcgattcg agttcggcaa
      421 gtcgccccaa gagaaagccg cgaaaattca cctccacgaa cagcgcaatg ccgcagatga
      481 accacggcac catgagaagg accggaagcc gaccatggag tgtttgtttc aggaaggtga
      541 tatcatagcc aagagacgga tagaccgaga gaatcacgaa ggtattcaga catcccagta
      601 ataatcctgt gatcatcccc cattttagcc cgtttcccgc cttcgctttt tccaatccca
      661 gttgaaccgg cacctgaact gtacgcgaag cccagaggcc catcgccaga taggcaagaa
      721 gttgcggcgc gaattgaacg aggagctctt ctcggagtgc gctgggaagc cagtagtaac
      781 agaatgtagt tccgatggga agcagcggca gcgctgctcc catgttgaac gtgtgatctg
      841 gacctggccg atccggcccg gaaaccatag gacggttacg aaagttcgag atgatagcga
      901 tccagcgtgg ttgctttatg ccgcgcgaga ttggacagcg acttgttgta atcgaggatg
      961 gcgcgcagct cgttgccttg tgcagtggcc aggtcacgtt ggaaatccag aacgaaacga
     1021 gtcgtgctga gcccgacctt caaccgttcc tgctcggctt gcagctgttt ctccgccatg
     1081 atgcgcgccg atcgggtggt ctcgatgcgt ttaaagtcgg tctgcacccg gcgcaccgct
     1141 tcacgcacgc cgacgatgat ttgctgacgc acactggcga gcgaagcctc ggcatttcgg
     1201 acatccagtt ttcgtttgtt gtacgtgttg atggcggccc ggttaccgag cggataacta
     1261 agcaccagcc ccgcgccata gttgtaaaag tcaccactga aatttctgct gaaggcctga
     1321 ccatagtcgc tccctaatcc ggcgagcccg atcgtacctt ggaatgagag ggtgggcagc
     1381 aactggttgc gggcgaattg cttattcagt tcccccgatt cgacgttttt cttcgcctgc
     1441 acgatttccg gccgctgttc gatcgccgta tcgatggctt cttggagact gaggggttca
     1501 agaaccgtga cggggggatc ggctggggtg aggcggacgt cttggcgcag gtcttcttcg
     1561 cccggattca agagacggcg cagttgatct tcttggtcgc gaatggcttt ttccgccact
     1621 aaggtctgct ccactctgga tgcgacggca gcctccgctt ggagcacgtc gacgatcgac
     1681 atgacgcccg ctttcgcctt ggcccggttc gacgccaaca gttcttcggc ggccttcatg
     1741 gcggcttgcg ccactttcaa gttctcattc gcaaatacca gttcccagta ggtttgttcc
     1801 accgtggtga tgacggtcag tacgcggtct cgaaagacat gctgttcgac ctcggcatta
     1861 ttctgggcga ccttgatgaa ggttctgttg atctcgatcc cggcgttctt cagcaatggc
     1921 tgggtcaatg tgaatgccag gccgccggtc caggacggat tgaacaaaaa tcctctggcg
     1981 acattcgcat tcacattggt tcgagccggg ctataattca tatcgaaatt cccgcccgta
     2041 atgaggttgg tctgggcatc gaacgtaaac gattcgctgc gttgatcaaa ggctgtgatc
     2101 tgattaagcg aaccccctgt tcccccgaac acgggtcgat tgagcgggtt caccgtccgg
     2161 gcatattgcc cgttgaggct cacgttggga tcgaacttgg agtattcgat gataatgtcc
     2221 gccaaccggc tttctttatt gtggcggctg atactgatgt cgagattgtt ctgcagggcg
     2281 cgaaccgccg catcggcgag cgacacggtt tcacgccgct ccgaagagat ccgttgggtc
     2341 acatcgaggc cccaactaac gggcggagac aagatggtcc acaagcctgc gatcacgatg
     2401 gttgcaaggc ggtgataagt tggggaactg cgattcataa tttcctcctt gatccacaat
     2461 tcgagcgagt gcatactaat actatagtct gtctggtgaa tcgtgtcatg aacattacga
     2521 tgggtggtta tcttatcgat aaagccgtca ctgattctcg acgtgtgagt atcgtcgctg
     2581 ttctcctgca gacgctggca tttgcggcgc tcagcaccag cccgtgtgcc tttgcgcaga
     2641 atgcgtcggc ggcatcgggt gcatcgagcg tccgatcgtt tgccggcggg gtcgctccgt
     2701 tgttcacgtt tcctgggggc agtttgtaca tcgataatca gggcacgcaa gggtttctcc
     2761 acacgccagg acagaacttt gaatccttta gctttcgcaa tccgacgact ggtcaggcgt
     2821 ggagcggcgc cgcgatgacg ttcggtccgc aactgtccat cggtctcatc cagggtgcga
     2881 atcaggtagg aaactcaact gttcttccgg ggccaccacc tcaaacgtct ccgttgcctc
     2941 cgatcgagtc gggcattctc gacgagatcc cctaattcac tgagcgaaac ggctcttcgc
     3001 ttgaatcctc gtagtatgta gagacgcgat atcctgaagc cgtccactcc tttcactcac
     3061 tcgtaatgcg tcacggttga taggcaagtt gccacgccac ggccaagacg taccgggtca
     3121 ccgtttttaa gatttcggga tcgattgtct cggcggtatc gcttggttga tgaaagtgcg
     3181 gatggacgcc cccgctcacc accgtgactg tcggcacacc ggcctccttg aacggcacat
     3241 gatcccctcc ggggaagaat ccatagagat ccagtttttc gtgaacaccg gcggtcttcc
     3301 ctacctccag caggccttct ttttctacca cagtcacgcc tacagtgagt cgcccatttc
     3361 caaccccagc atggtcgatg ttaatcatgg ctttcgtcga attgagtgga acaacaggcc
     3421 gtgatgtata aagacgcgaa ccaagcagat cgcgctcctc gccgctaaag gagacgaaga
     3481 ggatggtccg ggagggacgc ggctcaactt tgctgagagc gcgagccact tccaagatca
     3541 ctgctgttcc ggaggcattg tcatctgcgc cggggaaaag cattccccct ggttgaccga
     3601 agtggtcccg atgggctccg atgatcacag actcggttcc ggttccaggg atcatcccta
     3661 tcacattcat caagatccct tccgccgtcg tggtcttcca ctgaagcgat gcaaaccgat
     3721 ccgtccgtgt tgcttgtgat gatgatgcct ggttcagtcg ttcttgcagg acacgcaggc
     3781 gatcgggcat gtctgtactc gatcctccaa gaagctcttt ggccagggct gtgctgaccc
     3841 aggcacctgg gatagcttga tcaaggggca attgtccgta gaaggcgctg gggctaccgt
     3901 gtatgccgcg gcggacttca taagggtgga gaatgggtcc cgttgccgtc agataaccga
     3961 tcgctccacg ctctcgtgca aaccgaacct tgtcggcgtg gctgacggct cgcgggtagt
     4021 gttccgcctt gccgcgaaga aacagcacga tgcaatcatt cacgtcgaca ccggcatagt
     4081 catcgatgcc ttgggcagga tcgacgatgc catatacgac gaagacgatc tgggcttgaa
     4141 ggtctgcgga gggcgaatcg aagacgg
//
LOCUS       DATMTI010000995         4028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_844595, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000995 DATMTI010000000
VERSION     DATMTI010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844595"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1058)
                     /locus_tag="VN039_15755"
     CDS             complement(60..1058)
                     /locus_tag="VN039_15755"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HWV47450.1"
                     /translation="MRAWLMDSYDGVEQLRLGEVPEPQPGPAEVLLKVGYAALNPADA
                     FLAQGLYPAKPTLPHILGRDGMGDVLQIGADVGNIRVGETVGILRCETGVETPGTLAE
                     KVVVPAESLVRIPAGWSHEEMAGAPLVFLTAWQALTQWNDPPAPPSEKSVLLVTGASG
                     GVGIASVLLGKSMNLIVVALSRDAQKRTKLKTLGADVVFDPEDKNLVKAISAAISPKK
                     IDLAVDCVGGKLFPQVIALLGYGGKISVVGRSGGTAPEFNTATLLFRRIRIGGVSVGD
                     YTAQAAQTAWKEIVHRLEMIGRRPLVDSIVSFEEVKKGFTRLAQGPMGKVVVRVAG"
     gene            1214..1645
                     /locus_tag="VN039_15760"
     CDS             1214..1645
                     /locus_tag="VN039_15760"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWV47451.1"
                     /translation="MSEEGNTEAVSGLRELAQRFRVCLILQFGSTVTGTTHDRSDLDL
                     AVQFETPHASFQAILEMQEALQARFPGCKVDLAILNRADPLFLKKIVESCRILFGMPQ
                     DLARLRLRAFKAYQDFRPYLELERRHVARRLSSLVAEPSRP"
     gene            1642..2178
                     /locus_tag="VN039_15765"
     CDS             1642..2178
                     /locus_tag="VN039_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HWV47452.1"
                     /translation="MIDRELITRKMSLILEDVAAMTKLSRLSRAQYLEDSINEALAER
                     YLERAIGRMIDINFHLITESGHAPPKDYHESFIRLGTLGVMTSDLAKETAMAAGLRNR
                     IVHEYDDIDPNPHDSRALLLQSRIRCCDKPGGERARRSVLQRPVSRTPQSFAAPHARL
                     ATRLHDFATNPRESCGLS"
     gene            complement(2234..>4028)
                     /locus_tag="VN039_15770"
     CDS             complement(2234..>4028)
                     /locus_tag="VN039_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV47453.1"
                     /translation="WEEFRPRLETFLTTQVDPNEIERTAKIPDSVLKGLFALGAFGMK
                     IPHQYGGLGFSYTNYGRALMLMASWSNALALTVAVPQSIGIAMPILLFGTEEQKCKYL
                     PIVAREAISAFALTEPMTGSDAANIATEAILDSAGTTFVVNGEKLWCTNGPIARYVTL
                     IARVPAKSIHQNGQTIWEPVPNDKGADDHVHTAFILDMQTPGVRVRQRCQFEGCRGIE
                     NAHMTFTDVHIPAGNVIGEVGRGLKYALTILNVGRAISIPAICLGMAKQAWQPTLDRA
                     NQRLTFKKPLGTRQTQRMRLGRMASNLFAMEALALTAWRMADQHTYDVRIEAALAKLF
                     CSERTIQFLKDAQVIFGGMGYETAHSKRLRGEPAFGIEQLVRDAEMYRIGEGATDILR
                     PFVAREGLDMHLELVGPLFDERTTAFHRLIELQRLLRFYVPWYVNQWKGSRLALRPEL
                     QHSNVRSKLIFVECASRRLARTIFYSMLLHGQALRDDQGRQNRIEMIGEDLLVIATTA
                     LYAEAQDRTAAHPEVWELVEECFRETKQRVEQDIKGLIRNQDTVVATVGEKALNDRYP
                     SLISGIIRRNLQDYSLERSPHHSCGNFSMKR"
BASE COUNT          842 a         1176 c         1106 g          904 t
ORIGIN      
        1 atgtatggtg tgattgcttg tcaggtcatg aacaatagag cgatgtcaaa tcaccgacat
       61 taaccagcca cccgcaccac taccttcccc atcgggccct gtgccaatcg tgtgaatcct
      121 ttcttgacct cttcgaatga aacgatgctg tctaccaatg gacggcgtcc gatcatctcc
      181 agccgatgga caatctcttt ccacgcagtc tgagctgcct gtgccgtata atccccgact
      241 gagacaccgc cgatgcgaat gcggcggaat aacaacgtcg ccgtattgaa ctccggagcc
      301 gtgcctccgc tcctgccgac gacgctgatc ttgccgccat aaccgagaag ggcaatgact
      361 tgagggaaga gtttgccacc gacgcagtcc acagccagat ctatcttctt cggactgatc
      421 gcagcggata tggccttcac tagattcttg tcctctggat cgaacacgac atcagctccg
      481 agcgtcttca gcttggttct cttctgtgca tcacgcgata aagcaaccac gatcaggttc
      541 atcgattttc caagcaacac tgacgcaatg ccgactcccc ctgacgcacc ggtcacgagg
      601 aggacggatt tttccgacgg aggggcaggt gggtcgttcc actgcgtgag ggcctgccag
      661 gcggttaaga aaaccaacgg ggcgccggcc atttcttcgt gagaccagcc tgcagggatt
      721 cggacgagac tttccgcagg cacgaccact ttctccgcga gagttccagg cgtttccact
      781 cccgtctcgc agcgaagaat cccgactgtt tcacccacac ggatgttccc tacatcagct
      841 cctatctgta gcacgtctcc catgccgtca cgcccaagga tgtgcggcag ggtgggtttg
      901 gccggataga gtccttgcgc caggaacgca tccgctggat tgagcgcggc gtatcccact
      961 ttgagcagca cctctgccgg gcctggctga ggttccggca cctcgccaag ccgcaactgt
     1021 tccactccat cataggagtc catcaaccag gcgcgcatag acttacctcc tcttagcgag
     1081 gctcaatggc ggtcacagtc attaccccat gcgtccgtaa tgatattaca acacacgcaa
     1141 caactggggc gcgtagacaa tgaagcgatt ctcagctagg ctacggattg ggtgaaaacc
     1201 ctgggagcgg ggaatgagcg aggaaggcaa cacagaagca gtgtcggggt tgagggaact
     1261 cgcccagcgt ttcagggttt gcttgatcct ccagtttggg tcaacggtca caggcacaac
     1321 ccatgatcgc agcgatctag acctggcggt tcaatttgag acacctcacg catcgtttca
     1381 ggccattctg gagatgcaag aggcattgca ggcgcggttc cccggatgca aggtagatct
     1441 ggctatcctc aaccgggcag accccctctt cctcaagaaa atcgttgagt cctgccgcat
     1501 cctgttcggg atgccccaag acctcgcccg cctgcgcctc cgtgccttca aagcgtacca
     1561 agactttcgg ccttacttgg aattggagcg ccggcatgtc gcccggcgat tgtcatcgct
     1621 ggtcgcggag ccttcccgtc catgattgac cgcgagctta tcaccagaaa gatgagcctc
     1681 attctcgagg acgtggccgc gatgaccaag ctatctcgat tgtcacgcgc gcagtatctg
     1741 gaggattcca tcaatgaagc cctggccgag cggtatctcg aacgggccat cggtcgcatg
     1801 atcgacatca attttcatct gatcaccgag tccggccacg cgccgcccaa ggactatcac
     1861 gaatccttta tacgccttgg cacgctcggc gtgatgacgt ccgatcttgc caaggagacg
     1921 gccatggccg ccgggctccg caaccggatt gtgcacgaat acgatgatat cgatcccaac
     1981 ccgcatgatt cacgagcgct tctgctgcaa tcgcggattc gctgctgcga caagcctggt
     2041 ggcgagcgtg ctcgccgctc agttcttcaa cgtcctgtgt caaggacgcc tcagtccttc
     2101 gcggctccgc atgcccgtct cgcgacgcga ctccacgatt tcgcgacgaa ccctcgtgaa
     2161 tcatgcgggc taagctagtg catgaagcct tgactgttgc ggtccgccgg attcccatgt
     2221 acttggacca cattcagcgc ttcattgaga agttgccgca ggaatgatga ggtgatcgtt
     2281 caagtgagta gtcttgaaga ttgcgtcgga tgattccgct gatgagtgaa gggtaccgat
     2341 cattgagggc tttttctccg accgtcgcca ccacggtatc ttgattgcgg atcagtccct
     2401 tgatgtcttg ttcgacacgt tgcttggttt ctcgaaagca ttcttctaca agttcccaaa
     2461 cctccggatg ggctgcagtc cggtcttgcg cttcggcgta cagcgcggtc gttgcgatca
     2521 ccaacaggtc ttcgccgatc atctcgatcc ggttttgccg accttgatcg tctcgaagcg
     2581 cttggccatg gagcagcatg gagtagaaga ttgtccgtgc gagccgccga ctcgcacatt
     2641 ctacgaagat caatttggag cggacattcg aatgttgaag ctccgggcga agcgctaatc
     2701 ggctgccttt ccattggttc acgtaccaag gaacataaaa tctcagaagg cgctgcaact
     2761 cgatgagtcg atgaaaggcc gtcgttcgtt cgtcgaagag gggtccgacc agctcaagat
     2821 gcatgtcgag cccctcgcgc gcgacgaatg gtcgtaaaat atcggtggca ccctctccga
     2881 tgcggtacat ctccgcatcg cggacgagtt gttcgatgcc gaacgcaggc tctccacgaa
     2941 gccgcttaga gtgggcggtt tcgtatccca ttcctccgaa gatgacttgg gcgtctttta
     3001 agaactgaat ggtcctctcg gagcagaaga gcttggccag ggccgcctcg attcggacat
     3061 cataggtatg ctgatctgcc attcgccacg cagtgagcgc gagggcctcc atagcaaaaa
     3121 ggttcgaagc catacggcct aacctcatgc gctgtgtttg tctggtcccg agaggtttct
     3181 tgaaggtgag ccgctgattg gcccgatcga gcgtcggttg ccaggcttgt ttggccatgc
     3241 ccaggcaaat cgccgggatg ctgatcgccc gacctacgtt gagaatggtc agggcatact
     3301 tgagcccacg acccacttca ccgatgacat tgcccgccgg gatgtgtaca tccgtgaaag
     3361 tcatgtgagc attctcgatg ccccggcagc cttcaaactg gcatcgctgc cgtacgcgga
     3421 caccaggggt ctgcatgtcg aggatgaagg cggtgtgtac atgatcgtct gctcctttgt
     3481 cattcggaac cggttcccat atggtttggc cattctggtg tatcgatttc gccggtaccc
     3541 gtgcgatcaa cgtcacgtaa cgggcaatgg gaccgttcgt gcaccacagt ttttcgccgt
     3601 tcacgacgaa cgtcgtcccg gcggaatcca agatagcctc ggtggcgatg ttggccgcgt
     3661 cagaaccggt catcggctcc gtgagagcaa aagctgagat ggcttctcgt gcgacgattg
     3721 gcaggtactt gcatttctgt tcctcggtcc cgaatagcag aatgggcatg gcaataccaa
     3781 tcgattgcgg cacggcgacg gtgagagcca gcgcattact ccagctcgcc atgagcatga
     3841 gggcacgacc ataattcgta tacgaaaatc cgagccctcc atattgatgg ggaattttca
     3901 tgccgaaggc gccgagcgca aagagccctt tcaataccga gtccgggatc ttcgccgtcc
     3961 gttcgatttc attgggatcg acctgggttg tgaggaatgt ttctaagcgc ggacggaatt
     4021 cttcccag
//
LOCUS       DATMTI010000996         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_36889, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000996 DATMTI010000000
VERSION     DATMTI010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36889"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /gene="exbB"
                     /locus_tag="VN039_15775"
     CDS             <1..438
                     /gene="exbB"
                     /locus_tag="VN039_15775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HWV47454.1"
                     /translation="VSMDALKDTIDYGVIGLLLALSVWSVAVAIERWLFYRRVNLSQF
                     SNAQLLEISLTKRLVIIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGTMAVNTIMIGL
                     SLALKATAVGLLVAIPCVVMNNVLRRRVSEILTHYKAQHGTEH"
     gene            422..796
                     /locus_tag="VN039_15780"
     CDS             422..796
                     /locus_tag="VN039_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWV47455.1"
                     /translation="MERNIDQINVIPLVDVMLVLLVIVLTTATFISTGQIPVNLAKAK
                     EAGDRKDVPIVISLTAEGSLFVNDVPVPDGGLPTALNTRPRESAVVVRADKVTLLEKF
                     VALVDEIRGLGFQQVSLEVIRL"
     gene            849..1133
                     /locus_tag="VN039_15785"
     CDS             849..1133
                     /locus_tag="VN039_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47456.1"
                     /translation="MAGVLSLAWRLRYWGYSFHATPATRAPSETVPVGCRDGGSVVLF
                     SRFYRGCKVKALHDSCIGTIHGDRRFKNKIGSAKNRNTGAVVFIAHRSDS"
     gene            1471..1743
                     /locus_tag="VN039_15790"
     CDS             1471..1743
                     /locus_tag="VN039_15790"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWV47457.1"
                     /translation="MANWIEDLNKRYPALLRTEGIQGKVTLTAMLHDDGLLSDVRVIK
                     SSGNRALDQVALEDVRNGPPIKLSRPLERPHMSVRFSISYDLKTVQ"
     gene            complement(1715..2074)
                     /locus_tag="VN039_15795"
     CDS             complement(1715..2074)
                     /locus_tag="VN039_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47458.1"
                     /translation="MKTTLRGSLRLLLVALVALFACSEFETAYFQDKVDHVTQNMVAG
                     RYGMPHKEERLQAGGSVWTYFDRGSGTVGYTGHAESSKCKAYLLTFDQSGVLRDWKQQ
                     DCTSQSGPITEPSSDHN"
     gene            complement(2127..>2650)
                     /locus_tag="VN039_15800"
     CDS             complement(2127..>2650)
                     /locus_tag="VN039_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWV47459.1"
                     /translation="FLPHKGMRVDIGGIGKGYAADRAVEEMKRAGAQGGVVALSGDIK
                     TFGLLPEGEGFLVGIKHPREDEALIAVIDLKDEAISTAGDYERFFERDGIRYHHILDP
                     RTLRPARGCQSVTVIAKEGAVADGLDTGIFVLGPTRGMELVERLPDVEAIIIDDKGTV
                     TVSSGLRDRLRAP"
BASE COUNT          569 a          818 c          662 g          601 t
ORIGIN      
        1 gtctcgatgg acgcgttgaa agataccatt gattacggag ttatcggttt gttactggcc
       61 ctaagcgtgt ggtccgtggc tgtcgccata gagcgctggc tcttttatcg acgcgtcaat
      121 ctctctcagt tctcaaacgc acaactgttg gagatctctc tgaccaaacg cctcgtaatt
      181 atcggcacgg tcgctgccaa cgccccatac atcggcctcc tgggaacggt tcttggtatc
      241 atgctgacat tccatacgat gggcacgtcg ggcacgatgg cggtgaacac catcatgatc
      301 ggtttgagtc tggccttgaa ggcgaccgcc gtcggtctat tggtcgcgat tccctgcgtc
      361 gtgatgaaca atgtgcttcg tcgtcgcgtg agcgaaatcc taactcacta taaggcacaa
      421 catggaacgg aacattgacc agatcaacgt gatcccgctc gtggacgtga tgttggtctt
      481 gctcgtcatt gtattgacca ccgctacctt catcagcacc gggcaaattc ccgtcaactt
      541 agccaaagcc aaagaggcgg gcgatcggaa agatgtcccg atcgtgattt cattgacggc
      601 agaagggagt ctctttgtca atgacgtgcc ggtccctgat ggaggtcttc ctaccgccct
      661 caacacccgg ccgcgtgaat ctgcggtggt cgtgcgagcg gacaaggtca cattgctcga
      721 aaagttcgtc gcgcttgtcg atgagattcg cggccttggt tttcagcagg tcagcctgga
      781 ggtcattcga ctgtaatgaa tgattccgtg tcgcttccgt ttgaacggac gagttcgatg
      841 gcgacaggat ggctggtgtc ctgtctcttg catggcggct tcgctattgg ggctattctt
      901 ttcacgcaac gcctgcaact cgcgccccaa gcgaaaccgt tccagtggga tgtcgcgatg
      961 gcggcagtgt cgtcctcttc tctcgattct atcgcggctg taaggtcaag gccctccacg
     1021 actcctgcat cggaacgatc cacggcgacc gccgattcaa gaacaagatc gggtcggcca
     1081 agaatcgaaa caccggtgcc gttgtcttca tcgcacaccg ctccgactca tgaacagcca
     1141 tccccgacca ctcacgcatc cccggacctg ctcccgcagt caccctcgct tgccgcagag
     1201 cctatggacc gatccgagca tgaaccgtta cctcacccgg tagcgacagg atcggaagct
     1261 cctggaccgc cggcacaggt ggagctgccg actcagcctt acccaatttc cggtcaacct
     1321 caaccgcccc tacagcatca gcgtgtttcg tcggctgaag atgaaataac ccagccgccg
     1381 tctatcgcat cagtatccgc tgtccctagc cctgcagata cggcggagtc caggccgact
     1441 cgtgcccagt atggatggct caccgacctt atggccaact ggatcgagga tctgaataaa
     1501 cgttatccag ccctgttgcg aacggaaggc attcagggga aggtgacgct gactgcgatg
     1561 cttcacgacg acggactcct aagcgatgtg cgcgtcatca agagctcagg caaccgggcg
     1621 ctcgatcaag tcgccttgga agacgtccga aacggtcctc ccatcaaact ttcccgccca
     1681 ttagaacggc ctcacatgtc ggtgaggttt tccatcagtt atgatctgaa gacggttcag
     1741 taatcggccc ggattgagac gtgcagtctt gttgtttcca atcgcgcaat acgcccgatt
     1801 gatcgaacgt caggagatac gctttgcact tgctcgactc cgcatgacct gtatacccga
     1861 ccgtgccgct gcctcgatca aagtaggtcc agaccgatcc ccccgcctgc aatctctcct
     1921 ccttgtgcgg cataccgtac cggccggcca ccatgttctg tgtaacgtgg tccaccttgt
     1981 cttggaaata tgccgtttca aactcgctgc aggcgaacag ggctaccaac gcaacaagca
     2041 acagacgcaa cgatcctcgt aaagttgttt tcattgtgtc gtcctcatcc tgtgttgatc
     2101 atcataacgg cacatatagg cacttctcat ggcgcgcgca gccgatcacg gagtcctgac
     2161 gaaacggtca ccgtcccttt gtcgtcaatg atgatcgcct caacatccgg taagcgttcg
     2221 accaactcca taccgcgcgt tggacccagc acaaagattc ctgtatccaa cccatcggcc
     2281 accgccccct ccttggcgat gaccgtgaca ctttgacaac cacgggccgg ccgcagggtc
     2341 cgagggtcca aaatgtgatg ataccgaatt ccatcccgtt caaaaaaccg ttcgtaatca
     2401 cctgccgttg aaattgcctc gtcctttaaa tcgatgacgg caatcaacgc ctcatcttca
     2461 cgtggatgct tgatccccac gagaaaccct tcaccctcgg gaaggaggcc gaaggtttta
     2521 atatctcccg acaaggcgac caccccaccc tgagctcctg cccgtttcat ctcctctacc
     2581 gcccgatctg ccgcgtaccc ttttccaatc cccccgatat cgacacgcat tcccttgtgt
     2641 ggaagaaaaa
//
LOCUS       DATMTI010000997         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_419411, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000997 DATMTI010000000
VERSION     DATMTI010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419411"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VN039_15805"
     CDS             complement(<1..895)
                     /locus_tag="VN039_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47460.1"
                     /translation="MPAGIISTGSHPKLLWPGIHAVWGQMYAEHTKEFPELYDVLDSD
                     QAYEEDIQVTGFGLASLKTEGASGTYDSEIQGILTRYVHLAYSLGYIVTYEELRDNLY
                     EQVSTRRAKANAFSMNQTVENVAAFLYNNAFVSTYYTTGDGVALISASHTQATGGTFS
                     NMLNPAADLSEASLEDMCIQIMGAQTDRGLLINIMPESLHVARQEWFNANRIMKSVLQ
                     PGTANNDINVLHATNAFPKGIKLNHYFSSPHAWFVRTNCPNGMQMFWREEPMFSQDND
                     FDTKNAKALAYMRFSVGATDPR"
     gene            complement(911..1540)
                     /locus_tag="VN039_15810"
     CDS             complement(911..1540)
                     /locus_tag="VN039_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47461.1"
                     /translation="MANQNQPSGLAPVRYRNGSPWNGAVNFYSIAAADTNAYWIGDPV
                     STLVSTAGTPGGDSFGIPNVTLWAGATVPVRGVIVAIGTNPQGGPFINPNNLTQIYRP
                     SGAAAVPYYVAVVDDPDVLFEIQEAGVHAVLTAASISRNVGLNTGTRSGTLLLSPTYL
                     DNNTVSTTATLQLKIQAAIQRADNTPFTQYQKWLVSINNHEFSAGTASL"
     gene            complement(1543..2028)
                     /locus_tag="VN039_15815"
     CDS             complement(1543..2028)
                     /locus_tag="VN039_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47462.1"
                     /translation="MEFTKSNPSNVAPKGLPEREYTSMSLPSLKLAVPEIPGYHCHWM
                     RGDAQRIHQAHQAGYEFVEKDEIELNRFSLAGDPSEEPGSDLGSRVSVYGDANGTRLY
                     LMKLKDEIWERYEQQKAIGQERIAGQLRGDKGFVAPGDDASNRYSKSNNRNLFQPNRR
                     A"
     gene            complement(2031..>2827)
                     /locus_tag="VN039_15820"
     CDS             complement(2031..>2827)
                     /locus_tag="VN039_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47463.1"
                     /translation="NLGWVPQEEFRGDASRWISAEAFVERGEHIMPILKANNERLQSE
                     VAGLRGQLSQFQLAIQEGRESIEELRKFHTEDTERKVQLARKNLLAELKAAKKEGDTD
                     REVDLQGELSELDSALREEKTTPVTPVQRQQPAIDPEFIAWQTTNPWFGVDKKRTALM
                     TASAEELRADPKNNTLLGRRFYEAAAAEASRILDGSSKRSSKVEVGGPSGGSASGPVG
                     RSYSDLPAEAKEVCERQSAKLVGENRAFKTKAEWQKHYVEQFFAGE"
BASE COUNT          664 a          740 c          730 g          693 t
ORIGIN      
        1 cgcgaggatc ggtagctccg accgagaacc gcatgtaggc caaagcctta gcattcttcg
       61 tgtcgaagtc gttgtcctgc gagaacatcg gctcttcccg ccagaacatt tgcataccgt
      121 tcgggcagtt agtacggacg aaccaagcgt gcggggagct gaaatagtga ttcagcttga
      181 tccccttcgg aaaggcattc gtcgcatgca ggacgttaat gtcgttgttt gcggttcctg
      241 gttgcaacac cgacttcatg atccgattcg cattgaacca ttcctgacga gccacgtgca
      301 aactttccgg catgatgttg attagaagac cacgatcagt ctgtgcgccc atgatctgaa
      361 tgcacatgtc ttcgagagaa gcctccgaaa ggtccgccgc cggattaagc atgtttgaga
      421 acgtgccacc cgttgcctgt gtatgggagg ccgaaatcaa cgctacgcca tcaccagtag
      481 tgtagtaggt cgaaacaaag gcattgttgt aaagaaaggc agcaacattt tccactgtct
      541 gattcataga aaacgcattt gccttggcac ggcgggtaga gacctgttcg tagaggttat
      601 ctcggagttc ctcataagtc acaatgtaac caagagaata cgccaagtgg acatatctag
      661 tcaggatacc ttgaatctcc gagtcatagg tacccgaggc accttctgtc ttgagagaag
      721 caaggccaaa cccagtgact tgaatgtctt cttcataggc ttggtccgag tccagcacat
      781 cgtagagttc aggaaactcc ttggtgtgct ccgcatacat ttgaccccag accgcatgaa
      841 tcccaggcca aagcagtttc gggtgggaac cggtactaat aattccagca ggcatgattg
      901 ttctcctttc ttacagcgag gcagtgccag ctgagaattc gtgattattg atcgacacca
      961 gccacttttg gtattgcgta aagggcgtgt tgtcggcgcg ctggatggca gcttgaatct
     1021 tcagctgcag ggtagcagtt gtcgagacag tattgttatc cagatacgtg gggctgagga
     1081 gcaaagtccc cgagcgagtt cccgtgttga ggccgacatt ccgggagatg gaagcagcag
     1141 tcaggactgc gtgcacacca gcttcttgaa tctcaaacag aacatcgggg tcatctacta
     1201 cggcgacata gtaaggaacc gctgcagcac cgctcggccg atagatctga gttagattat
     1261 tcggattgat gaacggaccg ccttgtggat tcgtaccgat agcaacgatg acaccacgaa
     1321 cagggaccgt agcacctgcc caaagagtca cgtttggaat accgaagcta tcgccaccgg
     1381 gagttcctgc agttgacaca agagttgaca ctggatcgcc aatccagtaa gcattggtat
     1441 ccgcggctgc gatgctgtag aagtttacag caccgttcca cggagaacca ttccgatagc
     1501 gaacaggggc gagcccgctc ggctggtttt gattggccat tattaggccc tcctatttgg
     1561 ttgaaagagg tttcgattgt ttgacttaga gtacctgtta cttgcatcat caccaggggc
     1621 aacaaagccc ttgtcacccc gaagttgacc agcgatacgc tcctggccaa tagctttctg
     1681 ttgttcatac ctttcccaaa tctcatcttt gagcttcatg agatagagac gagtcccgtt
     1741 ggcgtctcca tagacactca cccgagaacc aagatcggaa ccaggctcct cggatgggtc
     1801 acctgcaaga gaaaatcgat tcaattcaat ttcatccttc tcgacgaatt catagcccgc
     1861 ttggtgagct tggtggatac gttgagcatc tcctcgcatc cagtggcaat gataacccgg
     1921 gatttccggg acggccagct tgaggctagg aaggctcatc gaggtgtatt cacgctctgg
     1981 aaggccctta ggtgcgacat tagaaggatt cgattttgtg aattccatga ttactcccca
     2041 gcaaaaaatt gctcaacgta gtgtttttgc cattcagcct ttgtcttgaa ggcgcgattt
     2101 tcgccgacaa gtttagcgga ttgacgctcg catacttcct tggcttcagc aggaagatcg
     2161 gaataggaac ggccgacagg gccactagcg ctccctcccg aagggccacc aacctcgacc
     2221 ttagagcttc ttttgctgct gccatcgagg atgcgagaag cttcggccgc agctgcttcg
     2281 tagaaacggc gcccgagtag ggtattattc ttcgggtcag cccggagttc ctcagcagaa
     2341 gctgtcataa gggcagtacg tttcttgtcc acgccgaacc aggggttggt agtttgccag
     2401 gcaataaatt ccgggtcaat ggcaggctgc tggcgttgaa ctggagtgac tggagtagtc
     2461 ttctcctccc gcaacgcact gtcgagttcg ctaagctctc cttgaaggtc aacttcccga
     2521 tctgtgtcac cctccttctt tgcggctttc agctcagcaa ggagattctt tcgggctaac
     2581 tgaactttcc gctcagtgtc ttccgtgtga aacttccgaa gttcttcgat tgactcccta
     2641 ccctcctgaa tggcaagctg aaattgggag agttgtccgc gcagccccgc aacctcgctt
     2701 tgcaatctct cattgtttgc tttgaggata ggcataatat gctcacccct ctccacgaag
     2761 gcttccgcag aaatccaccg cgaggcgtct ccgcgaaatt cttcctgagg aacccaacca
     2821 aggttgc
//
LOCUS       DATMTI010000998         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_844771, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000998 DATMTI010000000
VERSION     DATMTI010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844771"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VN039_15825"
     CDS             <1..572
                     /locus_tag="VN039_15825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWV47464.1"
                     /translation="SLGACSWVPKGDGQLDVGIKDRGVASWYGEQFHGRQAANGELFD
                     MEALTAAHRTMPLGSIVRVVNLTNGKHLYVRITDRGPYEKGRILDLSHGAAVRLGMEH
                     GGVAHVQVEIVGERRPELILLSEKFSDRAGSLLADTASQTDRTSTGMASPARNLPGDL
                     WIARRNRWALAMLAVSHQADTPVASLVLS"
     gene            770..985
                     /locus_tag="VN039_15830"
     CDS             770..985
                     /locus_tag="VN039_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47465.1"
                     /translation="MAKKRAAEPDEAKLKKKIAAKLTNHSNPEGDAALRSLRKRLRRE
                     QRKRRALAQRKKRAAGSKGAAATPASA"
     gene            1023..>1517
                     /locus_tag="VN039_15835"
     CDS             1023..>1517
                     /locus_tag="VN039_15835"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV47466.1"
                     /translation="MEQEPKHSPRSDSPLSRQSGEEPIADAVADQVQADSHAAETLAV
                     GQNTVALDEEQVKDEIDIQIDLLSDSDWVVRREAAITLGEMGDERCVEPLARALRDGD
                     WQVREVAIEGLGQVGSPAVETLLKLLRDWEVRKYVIAALGKIRDERVLDPLILQLRND
                     EFKDD"
BASE COUNT          338 a          392 c          474 g          313 t
ORIGIN      
        1 tgtcgttagg ggcctgttct tgggttccta aaggggatgg gcaactggat gtggggatca
       61 aagatcgagg agttgcttcg tggtacggtg agcagtttca tggccgacaa gcggcgaacg
      121 gcgagctgtt cgatatggaa gcgctgacgg ccgctcatcg aaccatgccg cttgggagta
      181 ttgtgcgcgt ggtcaatttg acgaacggta aacatctcta tgtgcggatt accgaccgag
      241 gtccctatga gaagggccga attcttgatc tttcccacgg tgcggccgtc cgattgggta
      301 tggagcacgg gggggtggcg catgtgcagg ttgagattgt cggcgaacgc cgcccggagc
      361 tgatcttgtt gtcggaaaaa ttctcagacc gagctggttc acttctcgct gatacggcgt
      421 cgcagaccga tcggacgtca actgggatgg cctctccagc tcgaaatctc cccggcgatc
      481 tctggattgc aagacggaat cgttgggccc ttgcaatgct cgcggtgtct catcaggccg
      541 atactccagt cgcgtcgttg gtcctcagct gagttccatc ttgctgacgg catcgagcgg
      601 gcctcccggc ctgctttttc tatcgagacc cccacgctgt atttcatcga gcaatgacga
      661 tcgtgctgat cctatccaag ttgacacggt gcaaagctct ccactagact gcacctttct
      721 cgtcggcagc cgacaatatg tgagcgtatc gaaggggagg catggatcga tggccaagaa
      781 gcgggcagcg gaaccggatg aagcgaagtt gaagaagaag atcgccgcaa agctgaccaa
      841 ccacagcaat ccggagggag acgccgcgct ccggtccttg aggaaacgat tgaggagaga
      901 gcagcgtaaa cggcgagccc ttgcacagcg aaagaagcga gcggctggaa gcaaaggtgc
      961 ggcggcaacc cctgcgtcgg catagtcttt cggaagttac ggtcgtgtca ggtactgatt
     1021 ccatggaaca agaaccgaaa cattcccctc gaagcgacag ccctctcagc cggcaatccg
     1081 gtgaagagcc catcgccgat gcagtggcag atcaggtaca ggctgattct cacgctgctg
     1141 aaacgctggc agtcggccaa aacactgtcg cgcttgacga agagcaggta aaggatgaga
     1201 tcgacatcca gatcgatctg ctgagtgact ccgactgggt ggtccgtcgc gaagcagcca
     1261 ttaccctggg tgagatgggg gatgagcgat gtgtcgaacc attggcccgc gcattgcgcg
     1321 atggtgattg gcaggtccga gaagtcgcga tcgaagggct ggggcaggtg ggttctccag
     1381 ccgttgaaac tctcctcaaa ctgctccgtg actgggaagt tcgtaaatac gtcatcgcgg
     1441 cgcttggaaa aatccgtgac gaacgggtgc tcgatcccct aatacttcag cttcggaacg
     1501 acgagttcaa agatgat
//
LOCUS       DATMTI010000999         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_547264, whole
            genome shotgun sequence.
ACCESSION   DATMTI010000999 DATMTI010000000
VERSION     DATMTI010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547264"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VN039_15840"
     CDS             <1..217
                     /locus_tag="VN039_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47467.1"
                     /translation="TTCFQATSSSSYVAPDTQTSCPPDYTQSKDGTQCEKQQVQKIQA
                     VQEYYCDDGWELLDVESSGEQCVQKND"
     gene            406..2106
                     /locus_tag="VN039_15845"
     CDS             406..2106
                     /locus_tag="VN039_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47468.1"
                     /translation="MTIMLNKLKKHATAVVSGDVPPAITFLDPEKTFENTVDSLRDFI
                     IALDKEKADHLRYKVDGDVVKIFITPYRTTITQDDLEFSQGDYNVELVLALGVRDETH
                     LDKALEAHGKILHDATVATISAGPEVSNLGSIDWHDDSASSLSEMLVSLSEAIKGDKP
                     LLDEQISTALLTGIVAATDRFSNERTSSKVMTMAAQLMAAGANQQLIATKLEEANEIA
                     PEPLEVTEASKDDKPAEDKPQNNDGTTTLDEDTSTKLPKKKDSKPKKEADGTLAISHE
                     KEGDLDEVAEQVAEEEGKKATEEAEATLSKLDQTAKETFQENEEEAAKEAERKLAEEL
                     KAVAPAAASTEPQNEPMLGGTLNATTEEAAEQKRRDEDDDRNRTILSHGETGHVDRNA
                     FPETPPPLNATMQQEEETTAPDILSQPVSASNPPYKPEPYVAPLSASLPPAQESPVIN
                     PAPAPDDASEQVTPQPPTPAQTLADIDATMRQPHEEARAAIDAAFDAAPATEPTAPSI
                     PGLPPMPDFSTLPPAPPAPSIPDLPPVEGMPPAAPMMDQPLQQPTASDPGQFKIPGQS
                     "
     gene            complement(2103..2789)
                     /locus_tag="VN039_15850"
     CDS             complement(2103..2789)
                     /locus_tag="VN039_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWV47469.1"
                     /translation="MRKIIFGIFAHPDDEAFGPSGALLLETKAGNDVHLITLTLGDAG
                     TNPDDVPDLAAVREKEWREAGRLIGAKSMRFLGYKDGQLHNLAMLEIYEKLIVLVSET
                     IKEEVDDIEIEFMSMDTNGITGHIDHIVAGRSASYAFYKLKETDPRITRLRLACIPDS
                     QLAEPNVLWLYMDAGRKENEIGEVVDAREHRDEIIAIMRAHHSQRADGESHINGNAER
                     IGLNYFIVKD"
BASE COUNT          736 a          776 c          742 g          545 t
ORIGIN      
        1 gacaacttgc ttccaggcaa catcgagttc aagctatgtg gcgccagaca cacagaccag
       61 ttgtccacct gactacacgc agtctaaaga cggaacccaa tgcgagaaac agcaagttca
      121 aaagattcaa gctgttcagg agtactactg cgacgatggc tgggagctgc ttgacgttga
      181 aagtagcggc gagcagtgcg tccaaaagaa tgattgacta tggtaaaaca cttatggtat
      241 agtaatgcca aactcattaa aaataaacat aatcactagg aaaagaacat ggcagatgca
      301 ctcagcgcca gacaacaagt cgtcgacaag ataaaagaaa cgtcgaatat tttggtgacc
      361 gtcggagaca gtccttcggt tgacgaactg tcggccgcgc ttggcttgac gatcatgctc
      421 aacaagctca agaaacacgc cacggcggta gtaagtggtg atgtgccgcc ggccattacg
      481 ttcttggatc ctgagaagac gtttgagaac actgtcgaca gcttgcggga cttcattatc
      541 gccctcgata aagaaaaagc cgaccatctt cgttacaaag tggatggtga tgtcgtcaag
      601 atttttatta cgccataccg cacgacgatt acgcaggatg accttgagtt cagtcaaggg
      661 gactataatg tagagctggt tctggccctc ggagtgaggg atgaaaccca cctcgataag
      721 gcgttggaag cgcatggcaa gatcctacac gatgcgaccg ttgcgacgat cagcgcgggt
      781 ccggaggtga gcaacctcgg tagtatcgat tggcatgacg acagtgccag tagcctgagc
      841 gaaatgctcg tcagcctgag cgaagccata aaaggtgata agcccctgct tgatgaacag
      901 atatcgacgg cactgcttac cggtatcgtt gccgcgaccg accgcttcag caacgaacgc
      961 accagctcga aggtcatgac catggccgcc cagcttatgg cagccggtgc taaccaacag
     1021 cttatcgcga ccaaactgga agaagccaac gagatcgcac cggaacctct cgaagtgacc
     1081 gaagcttcca aggatgacaa gcctgctgag gacaaaccac agaacaatga cggaaccacg
     1141 acgcttgacg aagacacgtc gacgaagctt ccgaagaaaa aagacagcaa accgaaaaaa
     1201 gaggccgatg gcacgcttgc tatctcgcat gaaaaagaag gcgatcttga tgaagtcgcc
     1261 gagcaagtcg ccgaagagga aggcaagaag gccaccgagg aagccgaagc gacgttgagt
     1321 aaacttgatc agacggccaa ggaaaccttc caggaaaacg aagaagaggc agcaaaagaa
     1381 gcggagcgca aactagctga agaactgaaa gcggtcgctc ctgcggcagc gtcgaccgaa
     1441 ccgcaaaacg agccgatgct cggcggtacg ctgaatgcca ccaccgaaga agcagccgag
     1501 cagaagcgcc gcgacgaaga tgacgatcgc aatcgtacga tcttgagcca cggcgaaacc
     1561 ggtcacgtgg atcgcaatgc gtttcccgag acacctccgc cgctgaatgc gacgatgcag
     1621 caagaagaag agacgacagc cccggatatt ttgagccaac cggtctcggc tagcaacccg
     1681 ccgtacaagc ctgaaccgta tgtcgcgcca cttagcgcct ccttgccgcc agctcaagag
     1741 tctccggtga ttaatcctgc accggcaccg gacgacgcct cggagcaagt tacacctcag
     1801 ccgccaactc cagcgcagac tctagctgat atcgatgcga ccatgcgtca accgcatgaa
     1861 gaagcccgtg ccgccatcga tgccgctttt gatgccgcgc cagccacaga accgaccgca
     1921 ccatcgattc ctggcttgcc accgatgcct gatttctcga cgctaccacc agcgcctcca
     1981 gcgccgtcaa ttcccgacct gcctccagtc gagggtatgc ctccagccgc accgatgatg
     2041 gatcagccgc tgcagcaacc caccgcgagt gatcccggcc aattcaagat tcccggccaa
     2101 tcttaatctt taacgataaa gtagttgagg ccgatccgtt cggcgttgcc gttgatgtgg
     2161 ctctcaccat cggcacgctg tgagtggtgg gcacgcatga tagcgataat ctcatcgcgg
     2221 tgttcgcggg catcgacgac ttcaccgatt tcgttctctt tacggccggc atccatgtag
     2281 agccaaagga cgttcggttc ggcaagttga ctatcgggaa tgcaggccag acgcaagcgt
     2341 gtgatacgcg ggtccgtctc cttcagcttg tagaaggcat aactggcact tcgtccggcg
     2401 acgatatgat cgatatgacc ggtgatgccg ttggtgtcca tcgacatgaa ttcgatttca
     2461 atgtcgtcca cttcttcttt tattgtttcg ctcaccagga cgatcagttt ttcatatatc
     2521 tcaagcatgg ccaggttatg cagttggccg tctttatagc ccagaaaacg catgcttttc
     2581 gcaccgatca gcctaccggc ttcgcgccat tccttttcgc ggaccgcggc caggtccggc
     2641 acatcgtcgg gattcgtgcc ggcgtcacca agtgtaaggg taatcaaatg gacgtcgttg
     2701 ccggctttgg tctccaggag tagtgcgcca ctcggtccaa acgcctcatc atcagggtgg
     2761 gcaaagatgc cgaagataat ctttctcatg ctccttagt
//
LOCUS       DATMTI010001000         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_291558, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001000 DATMTI010000000
VERSION     DATMTI010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291558"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1322)
                     /locus_tag="VN039_15855"
     CDS             complement(120..1322)
                     /locus_tag="VN039_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWV47470.1"
                     /translation="MRIAQVSPLWESVPPKLYGGTERIVSYLTEELVRIGHDVTLFAS
                     GDSITAARLVPIWPEALRLSKDIAYRDAPVVLQQERVLGMAEEFDLIHFHMDMPGFPI
                     ARRCGTPTITTIHGRLDLPELRPVFHEFADMPLASISDAQRHPLPWANWVATVQHGLP
                     RDLYGFHPKRGKYLAFLGRITPEKRVDLAIEIAKRTGMRLGIASKVDPVDRAYFEAEV
                     EPLLHHPLIDYIGEINDAEKDKFLGDAEALICPYDSTEAFGLALIEALACGTPVITYD
                     HGPFPEIIEDGVTGFLCRSVRDMIEAVGLVPLIDRHRCRQQFEQRFTTERMTQDYVDV
                     YRQLVEPRRTAVCLPPHAVRISEARMDSPRGSLRGRKCDVMSTARGGPAQSAFASTAQ
                     PLPPSPLR"
     gene            complement(1673..3148)
                     /locus_tag="VN039_15860"
     CDS             complement(1673..3148)
                     /locus_tag="VN039_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HWV47471.1"
                     /translation="MFTIVPGAEVHAAGPIQHENVRPGTTDWQLTNPAELQEIEGYAS
                     LTSVNRGGKINLFVNTSDAFYEMDIYRMGWYGGAGARLVTGGITRKGVRQEMPDPEPE
                     TGLIECRWTDPYVLTTRNPQDDTDWLSGVYLVKLTGQSSGKQSYIIFVVREDDRPSEY
                     LFQTSVTTYQAYNNWGGKSLYGFNSMGRPARKVSFNRPYIMSENQQAAFGNGAGEFLT
                     NNSVPPSDRPSPAGWEYNMVRWLEREGYDVTYSTNIDTHADPTLFNTHKAWLSVGHDE
                     YWSWEMRAHVEQARDRGVGLAFFSANVCYWQIRLEPSAVTGEPHRTIVSYKEAGKDPY
                     ALDHDPANDHLITVRWREPPLNMPEESLIGVMLIGNPVDADLVIVDETHWALSGTGLR
                     GGERIPGLLGYEVDRLFERAPAGIDVIAHSPFPYEGATAYADTTAYTWPSGAIVFACG
                     TIQWSWALDDFNVPALRPSRLHPAAQQMTRNVLSRLAGHSF"
BASE COUNT          699 a          956 c          984 g          696 t
ORIGIN      
        1 aagcaagcac agcagaaaat ccgacatcaa gggttcagtc aatcccaaac aggtaataca
       61 gtggttactg agtagtcggg gggggaggta tgtctactgc aaaaggcgag tcgtatccat
      121 cacctcaatg gcgatggggg gagtggttga gcggtggagg caaaagcaga ctgagcaggc
      181 ccgccccgcg ccgtggacat gacgtcgcat ttcctgcctc gcaagctacc tctgggacta
      241 tccatcctag cctcagagat gcgcacggcg tggggcggca aacataccgc tgttcgtcgg
      301 ggctcaacca actgccggta cacgtccacg taatcctgcg tcatgcgttc cgtggtaaag
      361 cgctgctcga attgctgtcg gcatcgatgt cgatctatta ggggaaccag cccgaccgcc
      421 tcgatcatat cgcgcacact acggcataag aacccggtga cgccgtcctc gatgatctcc
      481 ggaaacggtc catgatcgta ggtaatgacc ggcgtgccgc aggccagagc ctcgatcaag
      541 gccaggccaa acgcttccgt cgaatcgtag ggacagatca atgcctcggc atcgccgaga
      601 aatttgtctt tctcggcgtc gttgatctcg ccgatgtagt cgatcaaggg gtgatggagc
      661 aacggttcca cttccgcctc gaagtaggcg cggtcgaccg gatcgacttt ggaggcaatc
      721 cccaagcgca tacccgtccg cttggcaatc tcgatggcca gatccacacg cttttcaggc
      781 gtgatgcgtc cgaggaacgc caaatacttc ccccgcttcg gatgaaagcc gtacagatcg
      841 cggggcaggc cgtgttgcac ggtcgccacc cagttcgccc aaggcagcgg gtgccgttgg
      901 gcgtcggaaa tggaggccag cggcatgtcg gcaaattcat ggaacaccgg ccgcagttcc
      961 ggcaagtcga gccggccatg tatggtggtg attgtcggcg tcccgcagcg ccgtgcgatc
     1021 ggaaaccccg gcatatccat atgaaaatga atgagatcga attcctcggc catgccgagc
     1081 acacgctctt gctgcagcac cacgggcgcg tcacggtacg ctatgtcctt agagagccgt
     1141 aaggcttccg gccagatcgg aaccagtcgt gccgcagtga tggagtcgcc gctggcaaag
     1201 agcgtgacat catgtccgat ccggaccagt tcctcggtga gataggagac gattcgttcg
     1261 gttcccccgt agagcttggg gggaacgctt tcccataacg gcgacacttg agcgattcgc
     1321 atccgttttt ccgttaaagc ccaaagcacc taccactgct gtcacccact gctccaagca
     1381 agcattaggc ctgttagaac ggaaggcggc tctatcttgt gggttgtggg ttctgaaagc
     1441 tctgggacag cgccgtctgg cggcggcggc gttttttgtc aggaggagaa ttcttaaatc
     1501 aagttgacca tccggcctgg ataggctccg ttgtgccgtg cggattgtgc gaaacaccgc
     1561 ggcttaaatc aagttgaccc tccggcctgg gcaggctcag tatcgcggcc tagacagagc
     1621 gtgtcccggg aaagacagtt tgtctcagta atatccaggg tcgcagatca ggtcaaaacg
     1681 aatgtccggc taatcttgaa agtacatttc gtgtcatctg ctgagcagcg ggatgcagac
     1741 gagaggggcg cagggccggg acgttgaagt catccaatgc ccagctccat tggatggtcc
     1801 cgcaggcaaa gacaatagcc ccgctgggcc atgtgtatgc ggtcgtatcg gcgtaggccg
     1861 tcgcgccttc atacgggaac ggcgaatgag cgatcacatc gattcccgcg ggagcgcgct
     1921 cgaacagacg gtcgacttca tagccgagca ggcctggaat gcgttcgcca ccgcggaggc
     1981 ccgtgcctga gagagcccaa tgggtctcat ctacaatgac aaggtccgcg tccacgggat
     2041 ttccgatgag catgaccccg atcagcgact cttccggcat gttcaacggc ggctcgcgcc
     2101 atcgaacggt gatcaaatga tcatttgccg gatcgtgatc gagcgcatac gggtctttgc
     2161 ccgcctcctt ataggagacg atcgtgcgat gcggctcacc agtgaccgcg ctgggttcca
     2221 gcctaatttg ccagtagcaa acgttcgcgg aaaaaaaagc gagcccgaca cctcgatccc
     2281 gcgcctgttc gacatgggcg cgcatctccc aagaccagta ttcgtcatgg ccgacggaca
     2341 gccacgcttt atgcgtgttg aacagggtcg gatcggcatg tgtgtcgatg ttggtgctgt
     2401 aggtcacgtc gtacccctcg cgttccagcc atcgcaccat gttgtattcc cacccggccg
     2461 gtgacggccg gtcgctgggc gggacggaat tgttggtgag aaactcccct gcgccattac
     2521 cgaatgcagc ctgctggttc tcactcatga tgtacggtcg gttgaacgac accttgcggg
     2581 cgggacggcc catgctgttg aatccgtaca gggacttgcc gccccaattg ttgtaggcct
     2641 gataggtggt cacgctcgtc tgaaagagat actcggaggg acggtcatcc tcgcggacga
     2701 cgaaaataat gtaactctgt ttcccgctgg actgaccggt gagtttgacc agatacaccc
     2761 cgctcaacca atcggtgtcg tcctgtggat tgcgtgttgt caagacatag ggatcggtcc
     2821 accggcattc gatcaatccg gtctcaggct cgggatcagg catctcttga cgcacaccct
     2881 tgcgcgtgat cccgccggtg accagccgcg cccctgcgcc gccgtaccat cccatgcgat
     2941 agatgtccat ttcataaaat gcgtcgctgg tattgacgaa gaggttgatc ttccctcctc
     3001 gattgacgct cgtgagagag gcatagcctt cgatctcctg gagttccgcc ggattggtca
     3061 gttgccagtc ggtggtgccg gggcgcacat tctcgtgttg aatggggccg gctgcatgga
     3121 cttcggcccc cggaacgatc gtgaacatca agagtactcc aataccgcac catgacaccg
     3181 acaccctcgc cttcatttgt aggttgagaa gctcatggat aagtgagtgg aaaaatggtt
     3241 gggtgatcat ccgtcagctc agccccacaa tggattccct cagcagagtg cgtttcccac
     3301 agttggttgt tccgtccagt tgtgcgagaa cggcc
//
LOCUS       DATMTI010001001         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_631407, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001001 DATMTI010000000
VERSION     DATMTI010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631407"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VN039_15865"
     CDS             <1..307
                     /locus_tag="VN039_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWV47472.1"
                     /translation="GFNSKVVVTGDITQVDLPSDRVSGLIQVKEILRDIEGIEFVYFD
                     EKDVVRHRLVQDIIKAYDRHQPVNSDDPRHARGQVTSPQRPSPNSRKSPPGLTSSSH"
     gene            307..801
                     /gene="ybeY"
                     /locus_tag="VN039_15870"
     CDS             307..801
                     /gene="ybeY"
                     /locus_tag="VN039_15870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWV47473.1"
                     /translation="MAVYLRVSLTAFTVRQSTLAHLAEHVLSVVGESRSELSLELTGD
                     SRMRRLNLEYRKNDHPTDVLAFPIREAVMPRGVCPLSPMLGDVVISLPTAVRQAREAG
                     RSIDDELAILLVHGVLHLCGYDHERNPREAARMLRRERALLHLISPVPRMVTFRAGRK
                     PRGR"
     gene            804..1739
                     /gene="ftsY"
                     /locus_tag="VN039_15875"
     CDS             804..1739
                     /gene="ftsY"
                     /locus_tag="VN039_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWV47474.1"
                     /translation="MGWFQRLSEGLGRTRNVVQQSLDRFLGRAPDEELLEDLEAALLA
                     ADLGARVVDRLMLQVRGETRGADANTSEGLQNVLSRTLYSILKTVSGPSIDQLVNESP
                     KPLVTLVVGVNGVGKTTTIAKIAQRMIQAGRRPLLVAGDTFRAAAIDQLQVWGNRIGV
                     EVIRQRHGADPAAVAFDGIVAAKARTVDMVLIDTAGRLHTKSNLMDELRKVKRVIAQE
                     LPGAPHEVLLVLDATVGQNALAQARQFHEAVGVTGLALTKLDGTARGGIVVAIAEELK
                     LPLRLIGVGEGADDLQDFNAEAFVAALFGQPTSHS"
     gene            1826..>2353
                     /locus_tag="VN039_15880"
     CDS             1826..>2353
                     /locus_tag="VN039_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV47475.1"
                     /translation="MIKVLIVDDDQMNCDLLQSVFTRQGCHVISTTSGREGLDLFRAH
                     NPRVTLLDLRMPEMDGLTVLKEIRAIDPQAPVIILGGGATEIQENQARALRATDFIRK
                     GLSLDVLVEAVNRVSKLPVPSTTAQPALGSGNVIEQIDESVLVVDDEPLLCDLLVRFL
                     SLRGYRAFGVKDGHEA"
BASE COUNT          513 a          625 c          676 g          539 t
ORIGIN      
        1 ggggttcaat tcaaaagttg tcgttaccgg cgatatcaca caagtcgatt taccaagcga
       61 tcgagtgtcc ggtctcattc aagtgaaaga aattctccga gacattgagg ggattgagtt
      121 cgtgtacttc gatgaaaagg atgtggtccg gcatcggctg gttcaagaca ttatcaaggc
      181 ctatgatcgt catcagccgg taaatagcga cgacccccgg catgctcgcg ggcaagtcac
      241 ctctccccag cgcccatcac ccaattcccg caagtcgccg cctggtctaa ccagcagttc
      301 tcattgatgg ctgtctatct tcgcgtgagc ctcacggcgt ttactgtccg acaaagcaca
      361 ctggcccacc tagccgaaca tgtcttatcg gtagtaggag agtctcgatc ggaactaagc
      421 ttggaattga ctggagacag ccgtatgcgc cggctgaacc tcgagtaccg caaaaatgat
      481 caccctactg atgtactggc ttttcccatc cgtgaggccg ttatgcctcg aggcgtatgt
      541 cctctatccc cgatgcttgg tgatgtcgtg atttcactac cgaccgccgt tcgccaagca
      601 agggaggccg gacgatcgat cgacgatgag ctggcgatcc tgttggtgca cggagtgctc
      661 catctctgcg gatacgatca tgaacgtaat ccacgagaag cggcgcgaat gttgcgtcgt
      721 gaacgcgctc tgcttcatct gatctctccc gtgcctcgca tggtcacgtt tcgtgctgga
      781 cggaaaccaa gaggtcgttg acgatgggat ggtttcaacg gctgagcgaa gggctcggca
      841 gaacacgcaa tgtcgtgcag cagtctctgg atcgattcct cggacgtgca ccggatgaag
      901 aacttcttga ggatttggag gcggcgctgc tcgccgctga cctgggcgcc cgcgtcgtcg
      961 atcgtttgat gctgcaggtc cggggagaga cgcgcggagc ggacgcgaac acttcggaag
     1021 gacttcagaa tgtattaagt cggacgctct atagtattct gaaaaccgta tcaggcccct
     1081 caatcgatca gctggtcaat gaaagcccta aaccgcttgt cacccttgtc gtcggagtca
     1141 acggcgtagg aaaaacgacc accatcgcca agattgcaca acggatgata caggccgggc
     1201 ggcgaccgct ccttgtcgca ggagacacct ttcgtgcagc cgccattgat cagcttcaag
     1261 tttgggggaa tcggatcgga gtggaggtga ttcgtcaacg acacggtgca gatccggctg
     1321 ccgtggcctt cgacggtatt gttgcagcca aagccaggac ggtggacatg gttctaattg
     1381 atacggcagg ccgcttgcac acaaagtcca atctgatgga cgagctgcgg aaagtcaaac
     1441 gggtgatcgc tcaggagttg cccggtgcgc cacatgaagt gttgttggtc ttggatgcca
     1501 cggtcggaca gaacgcgctg gcccaggccc gccaatttca tgaagcagtt ggggtcactg
     1561 gtcttgctct gacgaagctt gatgggactg cacgcggggg tatcgtcgtc gcgattgccg
     1621 aggaactgaa gcttcccttg cgcctaatcg gtgtgggcga aggagccgat gacctccagg
     1681 acttcaatgc cgaggcgttt gtcgcggctc tgttcggaca gccgacttcc cactcataaa
     1741 ccacggattg ccttctcatt attcacgctc gcgtgttatt cttttcccca ggtacagctt
     1801 tcaggttgag gaaaggtcgg cagcaatgat caaagtcttg atcgtggacg acgatcagat
     1861 gaattgcgat ctattgcaga gtgtatttac ccgacaaggg tgtcacgtca tttcgacgac
     1921 cagtggacga gaaggtcttg atctattccg tgcacacaat ccccgagtta ccctgctgga
     1981 tcttcgcatg cctgagatgg acggattgac tgttttaaag gaaatccggg ctatcgatcc
     2041 tcaggcaccg gtgattatcc tgggcggtgg agcaaccgaa attcaggaga atcaagcacg
     2101 cgctctgcgg gccacggatt tcatcagaaa aggattgtca ctggacgtcc tcgtcgaagc
     2161 cgtcaatcgg gtctcaaaac tacctgtacc gtcaacgact gcgcagcccg ctcttggcag
     2221 cgggaatgtc attgagcaga tcgatgaatc ggtcttggtg gtcgatgacg aacctctcct
     2281 gtgcgacctc ttggtccgtt ttctcagtct gcgcgggtat cgggcgtttg gtgtcaaaga
     2341 tggtcacgag gcc
//
LOCUS       DATMTI010001002         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_588676, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001002 DATMTI010000000
VERSION     DATMTI010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588676"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(602..1468)
                     /locus_tag="VN039_15885"
     CDS             complement(602..1468)
                     /locus_tag="VN039_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWV47476.1"
                     /translation="MKKIAQVMRANGRHWVGDGFPVRSLFFYGDDAQAISPFLLFDYA
                     GPHTFEPADSPRGVGQHPHRGFETVTIVYDGEVSHRDSTGAGGTIGSGDVQWMTAAGG
                     IIHEEFHSKAYTKTGGPFRMVQLWVNLPAKDKMASPGYQSITDADIPVVDLGQSVGRV
                     RVIAGNFAGTQGPARTFSPVNVWDVRLNRDADVTLDLPEGHTAMMATLSGRVTVNGSE
                     QAGEAEVIRFERDGSKVRIHADSDSIMLVLTGEPIKEPVFGHGPFVMNSEAEIRQAID
                     DFNRGRFGQVAH"
     gene            1595..1945
                     /locus_tag="VN039_15890"
     CDS             1595..1945
                     /locus_tag="VN039_15890"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV47477.1"
                     /translation="MTKQVGCPTEITLDVIAGRWKVMVIFWLLQDKRRFNQLQRDLSG
                     ITHRTLAKQLKEMEADGLVEREDFGEIPPRVEYRLTPLGRSLEPVLAAMHQWALQHGA
                     EIRRWTNESESVSE"
BASE COUNT          413 a          578 c          555 g          408 t
ORIGIN      
        1 gttcgagaca atacggatga agtccttgat gggaatgccg aagctcgtga cctgcgtggg
       61 ggccattcac ggactcttgt tcctgctcta aggcggccag ttgacggccg tcatcgaaga
      121 tgcgctccgc cagacactgg ctcgtcgtgc gatcaccaaa taaaccagaa agccggtgaa
      181 gctcccgata gtctgcgggc acggagttcg tcccggcgtg gatttgaacg attcggctgc
      241 cctgcgtgcc tttatggaac gacactgacc gcgactatca tcgtttcccc ggacttctgg
      301 tccgccatcc attggaatag cctttctcga taaacgtcac cgtttccgtc gtaggctcgc
      361 tccttccagc gctccagaag gtgaatacgc ttctgctgtt tctggcgatg gcacagacga
      421 gcagcgatgg agttcccgca gaccttgttt ctgctcatgg actgagaacg tcggttcagc
      481 aacagcagcc gcgtcgtcct tcccgcgttt cttcttcgcc aatccagctc tccattcggg
      541 ccacagccat cagcggcatc tactcgttgg tcgaggcacc tgtcggacga cgcggcgcaa
      601 ttcaatgagc gacctgtccg aaacggccgc ggttaaagtc gtcgatggct tggcggattt
      661 ccgcctcgct gttcatgaca aaggggccat ggccgaaaac cggctctttg atcggttcac
      721 ccgtcagcac cagcatgatg ctgtcgctat cggcatgaat tctgaccttc gaaccatcgc
      781 gctcgaagcg gatgacttcc gcttcccctg cctgttcgct gccattgacc gtcacacggc
      841 cggacaacgt cgccatcatc gctgtatggc cttcaggcag gtccagggtg acatcagcgt
      901 cacggttgag ccgcacatcc cagacattca ccgggctgaa tgtacgcgcc ggcccttgcg
      961 tacccgcaaa gttgcccgca atgacacgca ctcggccgac gctctggccg agatcgacaa
     1021 ccggaatgtc agcgtcggta atcgactggt agccaggaga cgccattttg tcctttgctg
     1081 gcaagttcac ccacaactgg accatccgga atggcccgcc ggtcttagta taggccttgg
     1141 aatgaaactc ctcgtgaata atgccgcctg cagcggtcat ccattgcaca tcaccggagc
     1201 cgatcgtgcc gcctgcaccc gtcgagtcac gatgtgaaac ctcaccgtcg tacacaatgg
     1261 tgacggtctc aaaaccgcga tggggatgct gcccgacacc gcgcgggctg tctgctggct
     1321 caaaggtgtg aggtccggca taatcaaaga gtaggaatgg gctgatagcc tgcgcatcgt
     1381 ctccatagaa gaagagcgag cggacgggaa agccgtcgcc gacccagtgg cggccattgg
     1441 cgcgcatgac ctgtgcaatc ttcttcatgt ttatacctcc aatgttacat gtcgagatga
     1501 aaatgcgcgc cgcgagagaa ttctccgacg cgcctgtgta aaatactaag cacggtacac
     1561 acacttctca agtacggtct tggaggatac tgtgatgacc aaacaagtcg gttgcccgac
     1621 tgaaatcacg ctggacgtga tcgcgggccg ttggaaggtg atggtcattt tttggttgct
     1681 ccaagacaaa cggcggttca atcagctcca acgtgacctg tccggcatca cgcatcggac
     1741 gcttgccaaa caactcaaag agatggaagc cgacggcttg gtggaacgag aggacttcgg
     1801 tgagatccct ccccgcgtcg agtatcggct gacgccgctc gggaggtcac tggagccggt
     1861 gctggcggct atgcaccaat gggcgctaca gcacggggcc gagatccgtc gttggacgaa
     1921 cgagagcgaa agcgtcagcg agtgagagcg gcat
//
LOCUS       DATMTI010001003         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_377928, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001003 DATMTI010000000
VERSION     DATMTI010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377928"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(737..964)
                     /locus_tag="VN039_15895"
     CDS             complement(737..964)
                     /locus_tag="VN039_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47478.1"
                     /translation="MTAYRVQYQGFTGTITKSFDSQEQAETWVAEKGLETVAVVKPAS
                     ELPAMVVTSTSCSPIVQYPTWPAHHRKGRSA"
BASE COUNT          352 a          380 c          417 g          358 t
ORIGIN      
        1 attggccgcg atatttggca tgttatatgg catgttgcag ctcgctagtc ggtgaggaag
       61 ggtaaatagg gggcgttgtc tggacaaggg gctcgatttc cattcggcaa ccgcaagggc
      121 tgccatcagt ctatgcgaac ccggttggag attggggaac acgactatgc cagaatacaa
      181 acagcgcaag attgaattaa ctcccaggcg atacgagaat ggggcctggc gttgcccgta
      241 tcggattttt gaatttagat cgacctgctg gggatatcac aaagggtgcc ctgacggtat
      301 cttcgcatca cgtcaagcgg ctgcagcggt agccctggag gaagcgaaat gtatcgtcga
      361 ttcgctcgat gtccccgtat agtgtcctca gtccgcacgc agtatgggtt tctgacctta
      421 cggacccagt aaccccacga gacaatcaaa aacctctgaa gccttgagac tccaggggga
      481 cgttgtggag ttcttgagaa gatctcggat cacgccgtca ggatacggcg gttcggctgg
      541 ccagcctaga cccgcgaata atgcggctat atcacttgcg tggtcattgt gcaagctggg
      601 cgagcgctag ctgtgggagg aaggaaagag ccttagagag atcgagctag cagtaaagct
      661 tcctgggcgg atgttgcgag cagcgggccg aggagatcat tcggggtgta gatgttatgg
      721 gtggttgagg tcgccattag gccgacctcc ccttccgatg atgcgccggc caggtcggat
      781 actgcacaat cggtgaacac gatgtagacg tgacaaccat cgccggcagt tctgaggccg
      841 gcttcaccac ggccaccgtt tccaatccct tctctgctac ccacgtttcc gcttgctcct
      901 gcgagtcaaa agatttcgta atcgtgccgg taaagccctg atattgcaca cggtaggcgg
      961 tcatgatgtc gttcctttct cagcagcggc gagcattttc aactgtacga taaaatcgta
     1021 cagttgaaga tcttccatga cgctcagcgg tcccggtgtg tggctcatgc ggtcctccct
     1081 ggctcagctt caatgacgac tctaacaatg gtatccagta agtattgtat gattgctgaa
     1141 tctcaatcgc aagcgccgtc cgttcgtcca gcaagcgaac gtcctgcatg agcatggtga
     1201 gcttaattag caaagtccgt ctcactcact ccctagccaa tttatcgaga ccatagagta
     1261 gttgatcaaa tattgtaaac ggtttagcga acacctcgct cttcgaagga tcaagggctt
     1321 ctttttcaac gggcaaatcc ataatctccg ctatcactgc cgagggacta aatagcgcca
     1381 gtagcaaaca cgttataacg attctcacat gcacctctca atatgtcctc tttcgcgcca
     1441 tcctttcgtc aataggagcg tagagtattg taggagggcg gtcagttgag gtgcaaggcg
     1501 ggagagc
//
LOCUS       DATMTI010001004         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_758795, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001004 DATMTI010000000
VERSION     DATMTI010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758795"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VN039_15900"
     CDS             complement(<1..416)
                     /locus_tag="VN039_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HWV47479.1"
                     /translation="MAHLVDIGSFKVGQGQRPFLIAGPCVIESEQLVMDTAGRVAELT
                     KTLGIPYIFKSSFDKANRTSIKSFRGPGIKDGLAILKKVKDHFALPVLTDVHTEEQAT
                     EAGKVVDILQIPAFLCRQTDLLIAAAQTGKVVNVKKG"
     gene            complement(510..>1828)
                     /locus_tag="VN039_15905"
     CDS             complement(510..>1828)
                     /locus_tag="VN039_15905"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWV47480.1"
                     /translation="HSVITKERRGDYLGGTVQVVPHVTDEIKQAIMRISKGVDVTIVE
                     IGGTVGDIESLPFLEAIRQMPYDVGRDNVLYVHLTLVPYIGTAGELKTKPTQHSVNKL
                     REIGIQPNILLCRTDRYIPPELKGKIAMFCNVDKDAVITAKDVETIYEVPIVFRKEGL
                     DELIVRQLHVETGQPNLREWDAMVQKIKRPKHEISIALVGKYVGLKECYKSLGEALVH
                     GGIDHETKVNINWIESEDIERQGTERILREADGILIPGGFGIRGIEGKVTTIRYARER
                     QVPFLGLCLGMQCAAIEFARNVAGLAGADSAEFDERSPHAVIHLMPDQHGVNDKGGTM
                     RLGSYLCKLGEGTLAQKMYGVSEIRERHRHRYEFNNSYREQLTAKGLVLSGVSPDGRL
                     VEIIELKDHPWFLGTQFHPEYSSRPHRPHPLFSGFVGAALRKKLGH"
BASE COUNT          393 a          558 c          446 g          431 t
ORIGIN      
        1 cccttcttca cgttcacgac ttttccggtt tgtgcggcgg caatcaggag atccgtctgc
       61 cgacagagaa aggcgggaat ctggaggata tccacaacct tgccggcctc cgtggcctgc
      121 tcttccgtat gcacatcggt caagacagga agcgcaaagt ggtccttcac tttttttaat
      181 attgccagac cgtctttgat cccaggacca cgaaacgact tgatggaagt acggttagcc
      241 ttgtcgaatg acgacttgaa gatgtagggg atccccagcg tcttcgtcag ctcggcgact
      301 cgccccgccg tgtccatcac cagctgctcg ctctcgatta cacaaggccc ggcgatcaga
      361 aacgggcgct gcccttgacc gactttgaag gaaccgatgt cgacaagatg agccatacct
      421 gtcacgtcca ggttaaggtt gaggttaagt gaagaattgc gcgcaccggc attggcatca
      481 ttcccgctac ctggccttaa ccttgacctt cagtgtccca gctttttccg caacgccgcc
      541 cccacaaacc cactgaaaag cggatgcgga cggtgcggac gggaactata ctcgggatgg
      601 aactgagttc ccaaaaacca cggatgatcc ttcaactcga taatttcgac cagtcgcccg
      661 tcaggagaga ctccactcag gaccagtccc ttcgccgtca gttgctcacg atacgaattg
      721 ttgaattcat agcgatggcg atggcgttca cggatctcgc tcacgccgta catcttctgt
      781 gcgagcgtcc cctctcccaa tttgcagagg tacgatccga gtcgcatggt tccccccttg
      841 tcgttgacgc catgctgatc gggcatgaga tggatcacgg catgaggcga ccgctcgtca
      901 aactcggcgc tgtcggcacc cgctagtccg gccacattcc gcgcaaattc aatcgcggca
      961 cattgcatcc ctaaacacaa accgaggaat ggaacctggc gttcccgtgc gtaccgaatg
     1021 gtcgtcactt ttccctcgat ccctcttatc ccaaagccac cggggatcaa gatgccgtca
     1081 gcctcccgca gaattcgctc cgtcccctgc cgttcgatat cttcggactc gatccaattg
     1141 atgttgacct tggtttcatg atcgattcca ccatgaacca gcgcttctcc caagctcttg
     1201 tagcattctt ttaaacccac atacttaccc accaacgcga tcgagatctc atgcttcgga
     1261 cgcttaatct tttggaccat cgcatcccac tcgcggagat tgggctggcc ggtctccaca
     1321 tggagctgac gaacgatcaa ttcgtccagc ccttcttttc tgaatacgat cggcacttcg
     1381 tagatcgtct cgacatcttt ggccgtgatg acggcatctt tatccacatt gcagaacatc
     1441 gcgatcttgc ccttgagctc cggaggaatg taccgatcgg ttcggcacaa cagaatgttg
     1501 ggctgaatac cgatttcacg aagtttgttc actgaatgtt gcgtcggctt ggtcttcagt
     1561 tcaccggccg tgccgatgta gggcactaag gtcaggtgga catacagcac attgtcacgc
     1621 ccgacgtcgt acggcatctg ccgaatggct tcgaggaacg gcaagctctc aatgtcgccg
     1681 accgtcccgc cgatctcgac gatcgtgaca tcgactccct tagagatccg catgatcgcc
     1741 tgtttgatct catccgtcac atgaggcacg acctggaccg tcccgccgag ataatctcca
     1801 cgacgctctt tcgtaatgac cgagtgat
//
LOCUS       DATMTI010001005         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_377955, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001005 DATMTI010000000
VERSION     DATMTI010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377955"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VN039_15910"
     CDS             complement(<1..660)
                     /locus_tag="VN039_15910"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HWV47481.1"
                     /translation="MSTIDSANPPQEYPDIVGARPTNPLAANFKPFIPASARLPELTW
                     PHLITGTILGIIFGASSLYLVLKVGLTVSASIPVAVLSITIFRIASRVFRTRDATILQ
                     NNIVQTAGSAGESIAFGLGVTMPAIMILGFELEITRAILVAVLGGLLGILMMIPLRRA
                     LIVAEHGVLKYPEGTACAEVLKAAVSKESIAVADKTHTTANAEIVNEEAARGGGKVIF
                     TG"
     gene            1017..>1615
                     /locus_tag="VN039_15915"
     CDS             1017..>1615
                     /locus_tag="VN039_15915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HWV47482.1"
                     /translation="MRKELTIRGLIIGIIITLVFTAANVYAGLKAALTFSTSIPAAVI
                     SMAILRSFRNATIQENNIVQTVASAAGTLSSIIFVLPGLIMIGYWSDFPFWTSFWICA
                     LGGVLGVMYSIPLRRALVTNSDLPYPEGVACAEVLKVGSSGETGEAKDVEHGRAGLLA
                     VLWGSIVSAVFYAIVQTKIFASDVAEYFRFGSRGSVTGF"
BASE COUNT          340 a          424 c          469 g          382 t
ORIGIN      
        1 gccggtgaag atgaccttcc cgccgcccct cgctgcttct tcgttgacga tttcggcatt
       61 cgcagtggtg tgtgttttat ctgcaaccgc gattgattcc ttcgagacgg ctgccttcag
      121 tacttcggca caagctgtgc cctcgggata tttcaaaaca ccgtgctcgg cgacaatcaa
      181 tgcgcgtcgc agcgggatca tcatcaggat tccgagtaat ccgcccagaa cggcgacgag
      241 aattgctcgc gtgatttcaa gctcgaatcc gagaatcatg atcgcaggca tcgtgacgcc
      301 gagaccgaac gcgatcgatt cacccgccga ccctgcggtt tgcacaatgt tgttttgcag
      361 gatcgttgcg tcgcgtgtcc ggaaaactct ggaggcgatc cggaagatgg tgattgaaag
      421 caccgctacg gggatcgacg cgctcacggt caatccgact ttgagtacaa ggtaaagtga
      481 tgaagcgccg aaaatgattc ccagaatggt gccggtaatc agatgcggcc acgtgagctc
      541 tggcaatcga gcggacgccg gaatgaaggg tttgaagttc gctgccagcg gattcgtcgg
      601 ccgcgcgccg acgatatccg ggtattcctg cgggggattt gcgctatcga tcgtcgacat
      661 gtgcgaatca gatcaggact cggtcgaaaa acacaacaaa caggtcgggc cgattttccg
      721 ccagggcgtt gaggcggggc gcctcgcctt ttgaaaacgg agaagggggg aagtgaagga
      781 ttattttcca cccgttggcc ataccgaggc tgacccctca cctaaatcct cttcccttgc
      841 aaggggagag gcgaacagcg gcggaaacag tgagcggaga agcgtcagtt acagtgtgtt
      901 gacaggccat gtcgcggttc ccaaccgctc cattctccct tgaggcagaa accggtccat
      961 cgattgatcg caatgccttg acccgttcgg tgattgcaca ctattctccg agtcctgtgc
     1021 gtaaagaact cacgatacgc ggtctcatca tcggcatcat tattacattg gtgttcactg
     1081 cagccaatgt gtacgccggg ctcaaagctg ccctcacttt ttcaacttca atcccggcgg
     1141 ccgtgatttc gatggccatt ctgcgcagtt ttcgcaacgc gacaattcag gaaaacaaca
     1201 tcgtgcaaac ggtcgcttcg gcagcgggca cgctctcgtc gatcattttt gttttgccag
     1261 gactaatcat gatcggttac tggagtgatt ttccgttctg gacctcattc tggatttgcg
     1321 cgcttggcgg ggttctcggt gtgatgtatt caatcccgct gcggcgcgcg ttagtgacaa
     1381 attcagattt gccgtacccg gagggagtcg cttgtgccga ggtattaaag gtcgggagca
     1441 gcggcgaaac gggagaagcg aaggatgttg agcacggccg cgctggtctg ctcgctgttt
     1501 tgtggggctc aatcgtttcg gcggtattct atgcgatcgt ccagacaaag atttttgcca
     1561 gcgacgtggc ggaatatttc cgtttcggca gcagaggttc cgtgactggt ttcga
//
LOCUS       DATMTI010001006         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_291790, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001006 DATMTI010000000
VERSION     DATMTI010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291790"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="VN039_15920"
     CDS             complement(<1..1783)
                     /locus_tag="VN039_15920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV47483.1"
                     /translation="MRDGTTAAIRPAEPTDVPMMQQFVDRLSPESRRHRFFSESPPPS
                     NSLAALCNFSNPHSQFTLVVTRVWKGTPRIIAAGSYWAKDGQTAEVAMAVDDEFHGKG
                     LGTLLLERLALVAIRQNFTHLWAVTHVDNLAMREVFRESGFTAHEAYEGNDMQVELSL
                     IPTETTVSRAEVRERLATTASLRPFFQPRSITIIGASRDPNKIGYRLLDALRTNRFRG
                     AVYPINPKATEIIGIRTYSSVLQIPEPVDLAVISVPRDSVSSVIDECAAKGIRACVII
                     TAGFAEVGSEGSTLQRQLVVKIRQHGMRMIGPNCFGILNTDPTIRLNATFSSLFPPRG
                     RVALSSQSGAIGIATLAGARRFHLGISSFVSVGNKADVSTNDLLQYWEEDPATNVILL
                     YVESFGNPRRFARIAQRVSHRKPIVAVKAGRTRSGRRAAGSHTAALAASDVAVDALFH
                     QAGVIRAETLDEMLALATGLATQPLPEGRRVAIITNAGGPAVLCTDACEAGALVIPEL
                     STQIKSTLAAFLPSAAALSNPVDLIASATPDQYAKAIEALLSADEIDALIILYMSVTV
                     ADTADIAHGILAGIENGRKAGAEAKPVL"
     gene            complement(1890..2747)
                     /locus_tag="VN039_15925"
     CDS             complement(1890..2747)
                     /locus_tag="VN039_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWV47484.1"
                     /translation="MRAVIGVDWSDQAFAAVTQTFQLYHPTDVTLVHGVDLGILKHPF
                     VAQAGNVQGYEEFRNAMVESGSQLLERAAAIVPAQVASIRKVNEIGSPAQLILDSAES
                     LSSDLVVVGVRGRSRLSEVVLGSVSHRVLLHSSRSVLIVRGAARKVQRVLVAIEDRDD
                     ADRIAKWLRHHPFVDPTELCVLHALVPIGVSDPYDALGTGTWWEGAERYANELVQSTA
                     GKLLNSRYTVSTNVATGNPAAVIEREAKGRDLVVVSSHGRKGLSRFLLGSVSHAVVHH
                     VTCPVLVVR"
     gene            complement(2874..>3356)
                     /locus_tag="VN039_15930"
     CDS             complement(2874..>3356)
                     /locus_tag="VN039_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47485.1"
                     /translation="NPEYALGAVGETGTIYLNPDALAVYNLSGDEMEALAQTQQREVA
                     RQQDLYRQGRQLPTLIDRTVVLVDDGIATGSTFFASAKSIKHLKPRRLIGAVPVGPVE
                     TIREACSQVDELIVLATPDPFWAVGNHYVDFAQINDADVVEYLKLAEEAMRERSQFSV
                     "
BASE COUNT          730 a          979 c          960 g          687 t
ORIGIN      
        1 tgagcaccgg tttagcctcg gcgcctgctt ttcggccatt ttcaattcca gctaagatac
       61 cgtgagcgat atcggctgta tccgccacag tcacggacat atagaggatg atcaacgcat
      121 cgatctcatc cgccgacaaa agagcttcaa tcgccttggc gtactgatcc ggcgtggcgg
      181 aagcaatcag atcgacggga ttgctcagcg cagcggctga tggcagaaag gcggcaagag
      241 tgctcttgat ttgggtggac agttctggaa tcaccaatgc tcctgcttca caagcgtcag
      301 tacagagaac ggccggccct cccgcattcg tgatgatcgc cacacgccgg ccttccggta
      361 agggctgagt cgcgagcccg gttgccagtg ccagcatttc atcgagcgtc tcggcgcgaa
      421 tgacaccggc ttgatgaaac aacgcatcaa cggccacatc acttgccgca agggccgccg
      481 tatgcgaacc ggcggcacgt ctccctgatc gggtgcgtcc cgctttgact gcaacgatag
      541 gtttacggtg actcacccgc tgtgcgatgc gtgcgaagcg gcgaggattg ccgaaggatt
      601 cgacataaag gagaatcacg ttcgtggctg ggtcttcttc ccaatattgc aacagatcgt
      661 tggtggacac atcggccttg ttccccacac tcacaaatga agagatgccc aagtggaacc
      721 gccgcgcacc ggcaagggtc gcaatcccga tggcgccact ttgcgacgac agggcgacac
      781 gacctcgcgg aggaaacagc gaggaaaaag tggcattgag cctgatggtc ggatccgtgt
      841 tcaatatccc gaaacaattg gggccgatca tgcgcatacc gtgctgccgg attttcacga
      901 ccaactgccg ctgaagggtc gatccctctg aacccacttc cgcgaagcca gccgtgatga
      961 tgacgcaggc ccggattcct tttgccgcac actcgtcgat cacggacgac acagagtctc
     1021 gcgggaccga gatgacagcc agatccactg gttcagggat ctgaagtact gatgaatagg
     1081 tccggattcc gataatttcg gtcgccttgg gattaatcgg gtaaacggct cctcgaaacc
     1141 ggttggttct tagggcatcg agcagccgat agccgatctt gttcggatca cgcgaggcac
     1201 cgatgatggt gatggaccga ggctggaaaa acggtcggag tgaggccgtc gtcgccagcc
     1261 gttctcggac ttcggcgcga gagacggtcg tttccgtggg aatcaatgac aactccactt
     1321 gcatgtcgtt gccttcatag gcttcatggg cagtaaaccc cgattctcgg aagacttcgc
     1381 gcatggccag attgtcaacg tgagtgacag cccaaaggtg agtaaagttc tgtcggatgg
     1441 cgactaaggc cagtcgctcc agcagcagag tacctaggcc ttttccatga aactcgtcgt
     1501 ccacggccat ggcgacttcc gccgtctgcc cgtcctttgc ccagtatgac cccgcggcaa
     1561 taatacgggg cgtgcccttc catactcgcg tcacgaccag cgtgaactgc gaatgtgggt
     1621 tggagaaatt gcacaaggcg gcaaggctgt tcgaaggcgg agggctttcc gagaaaaacc
     1681 tgtggcgtct cgattcaggg gataaccgat cgacgaactg ttgcatcatc ggcacatcgg
     1741 tcggttcagc cggtcggatc gcggcggtcg tcccatcacg catgatgagg gagccggact
     1801 cagtgtgatc ggcctccagg gggggaatgt aaagtggacg aacggatctc atcctagggc
     1861 gtctagtatc gctggcatcc tttattcctt taccgcacga caagcaccgg acaggtcacg
     1921 tgatgcacaa cggcatggga cacacttcct aaaagaaaac gagaaaggcc cttgcgccca
     1981 tgggagctga cgaccaccaa gtccctcccc ttcgcttccc gttcaatcac cgctgcgggg
     2041 ttgcccgtgg cgacgttcgt gctcactgta taacgagagt tcaagagttt gccggcggtc
     2101 gattggacaa gttcattcgc atagcgctcc gcgccctccc accatgttcc ggttcccagc
     2161 gcgtcatagg ggtcgcttac tccgatcgga acgagggcat gcagcacaca gagctcggtg
     2221 ggatccacaa acggatggtg cctcagccac ttcgcgattc ggtcggcgtc atctcgatcc
     2281 tcgatggcga cgagcacccg ttggaccttg cgagcggctc ctcggacaat caggaccgac
     2341 cgggagctgt gcaggagcac acgatgcgac acgctcccga gaacaacctc ggaaagacga
     2401 ctgcgcccgc gtacaccgac gacgacgaga tccgatgaga ggctttcggc gctgtcgagg
     2461 atgagctgag cgggactgcc gatttcattc accttcctga tcgacgcaac ctgtgccggt
     2521 actatggcgg cggcgcgctc cagcaattgg ctcccggatt cgaccatcgc attgcggaat
     2581 tcctcgtacc cttgcacgtt gcctgcttga gccacgaacg gatgtttcaa aatccctaaa
     2641 tcgacgccgt ggaccagcgt gacgtccgtc ggatgataga gttgaaacgt ctgggtgacg
     2701 gcagcgaacg cctgatccga ccaatcgacg ccgatgacgg ctctcatatc atggcctctc
     2761 ccatttgaac ctcaaaacag cggatgttga aggctacccc agtgcgccat tcgcagtctt
     2821 tagagctcat gcaccactgt cccttgaaaa caaagctcca tgtctccaag gatctacaca
     2881 gagaactgcg atctctcgcg catcgcttcc tcagccagct ttaggtactc caccacatcg
     2941 gcatcattga tttgtgcaaa gtcgacatag tggttaccga cggcccagaa cgggtccggc
     3001 gtggcaagga ctatcaactc gtccacctgg ctgcaagctt ctcgtatcgt ctccactggg
     3061 ccgactggaa cggctccaat caggcggcgc ggcttcaggt gcttgatgga cttggccgag
     3121 gcaaagaatg tggaaccggt agcgatgccg tcatccacga gaacgacagt tcggtcaatg
     3181 agcgtaggga gctgtcggcc ctgtcgatag aggtcctgct gtcgagccac ctctcgctgt
     3241 tgtgtctgag ccagggcctc catttcgtca ccggaaagat tatagacggc gagtgcgtca
     3301 gggttcagat agatcgtccc tgtttcaccc accgcgccta gcgcatattc gggatt
//
LOCUS       DATMTI010001007         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_758842, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001007 DATMTI010000000
VERSION     DATMTI010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758842"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..636)
                     /locus_tag="VN039_15935"
     CDS             complement(202..636)
                     /locus_tag="VN039_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV47486.1"
                     /translation="MKRMLPLRTLLVDDSPEFLGRLEKWLAGHPGLEIVGKAYSGLEA
                     VKEVSVLRPDLVVMDVAMPLMNGYEATVSIKEGAHRPMVILISFFDRRETCEDRDSKA
                     DAFLKKDSLYRDLLPTVARLFPQQRRQTESNFQTPERRGHGL"
     gene            complement(815..1468)
                     /locus_tag="VN039_15940"
     CDS             complement(815..1468)
                     /locus_tag="VN039_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV47487.1"
                     /translation="MSPLRVVIAEDHALVRAGMRALLQKIEGIEVVSDVGDGWEAVKS
                     VQTDTPDLVLMDIAMPGLNGLDATSRIVRESPTTRVILLSMHANEEYFQQALEVGASG
                     YLLKGAELAELELALKTVARGETYLTPAVAKYAIKAYREKSDGPSGPLTKLSMRQREI
                     LQLIAEGQTTKDIAQRLNLSVKTVETHRSQLMERLDIHDVPGLVRFAMRVGLIQLYS"
     gene            complement(1465..2919)
                     /locus_tag="VN039_15945"
     CDS             complement(1465..2919)
                     /locus_tag="VN039_15945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV47488.1"
                     /translation="MNSKYAQILIVLVFVVTFFIDTVSPMGYEVWVGHIFAVLLALWV
                     ERPNTPYIVAAIATILLILEFRLSPPGIPPDMAVFNRTVGVALLWITAGLVARAKTAK
                     LDDATRRLGAIVESSSDAILSVTPDGIVTSWNAGAERMFGYTAAEMLGRSILTLIPAD
                     LHDHEARLQASVQRGQSIRTDDAVRLTKDGRSIDVSVTLSPLRDAAGRFVGVSKIIRD
                     ISERKRAQTLLQQAREALEVKVQERTAELISANHALQKLSSRLMQVQEEERSRLARDL
                     HDEVGQLLTALKIDLQEVQHSEVAQVRSDFLTDGLELVDRLLTQVRTLALNLRPSILD
                     DLGLVPALRWYANRQADRNGWTLFFSAEEMTGRVSAPIEVACFRVAQEALTNVSKYAK
                     AKTIDLTLRRSNHRVTLVIQDDGTGFDVMLARRRAQGGQTIGLLGMEERVRLAGGAFE
                     IVSAPDQGTRVELCFPLGEQDPSKQFGTVEVMVP"
     gene            complement(3076..>3898)
                     /locus_tag="VN039_15950"
     CDS             complement(3076..>3898)
                     /locus_tag="VN039_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWV47489.1"
                     /translation="KMALGSFSAVEASVLRDLGGLAILLATWWSQEGGAIKISRADMG
                     LLGFLGLGVLGNHLLILMGLNYVSGAIGGVIIGSSPVVTALLSALLIRDVPLRAVWVG
                     ALLSFAGVGLVSVAGFQAAGEQPLLGSTLVFLGVVSWALYSIGSRTIMERISALTVNW
                     TTLMVATVLQIPLLWTDQKMIHAGIGSVMTSDWLALGYLILFATAVAQQAWLFGVKGI
                     GPSRASVLGNLTPVAAIGLSALILGESVGLIEIIGIGLILAGVWVVNRQTAESNN"
BASE COUNT          888 a         1216 c         1019 g          775 t
ORIGIN      
        1 ctccacatac tgttcaacgg cgtttcgttc cctgactatg ctgcgggagc agcggggacg
       61 gctcgcaccg ttcggttccg tatgatgacc gagtccacca catttccgca gttcacacag
      121 cgccaggacg atcccagaat aagttcatca ctcgcgctgt gccccgcacg ccgttcagac
      181 atcatccatc cgttgcatcg ctcacaaccc atggcccctc ctttccggcg tctgaaaatt
      241 ggattccgtt tgcctccgct gctgagggaa gaggcgagcc acggtgggga gcagatccct
      301 atacagcgag tccttcttga gaaatgcgtc ggctttcgaa tcacgatcct cacacgtttc
      361 acgcctgtcg aaaaatgaga tcaagatcac catcggccgg tgagcacctt ctttgatgct
      421 caccgtcgcc tcatatccgt tcataagcgg catcgccaca tccatcacca cgagatccgg
      481 acgaagtaca ctcacctcct tgacggcttc gagccccgaa taggccttcc ccacgatctc
      541 aaggccaggg tgacccgcaa gccacttttc caaccgtccg agaaactcgg gactgtcgtc
      601 cacaagaagc gtgcgcagcg gcaacatgcg tttcatcagc gcctcttcct tctcggttga
      661 tgggcaacgc attgtgcctc ggccctggtg tgcagcttgc caaaagagca ggcatggaca
      721 tatcgggaaa atcctgagag aaagggagaa atcccccgag gaagacgtac gagttcacgg
      781 aacgattgca ggaatacgaa ccaaccgaac agagttagga gtagagctgg atgagtccca
      841 cgcgcattgc gaaacggacg agtcccggca catcgtgaat atcgagccgc tccatcaatt
      901 gacttcggtg agtttcgacg gttttgacgc tcaagttcag gcgttgggcg atgtctttcg
      961 tcgtttgccc ttcggcgata agttgcagga tttctcgctg acgcatactg agcttcgtca
     1021 gcggaccaga gggcccatct gacttttctc ggtaggcttt gatcgcatac ttggcaaccg
     1081 ccggggtcag gtaggtctca cccctggcga ccgtcttgag ggccaactcg agttcagcca
     1141 gctctgcgcc tttcaacaaa tacccggacg cgccgacctc taacgcttgc tggaagtatt
     1201 cttcattggc atgcatcgac agcagaatca ctcgcgtggt cggcgattcc ctaacgatcc
     1261 tcgaggtcgc gtcgagtccg ttcaatcccg gcatggcgat atccatcagc acgagatcag
     1321 gagtatccgt ctgcacagac ttaacggctt cccacccatc cccgacatcg gaaacgacct
     1381 caatcccttc gattttttgc aagagggccc gcataccggc tctgaccaac gcgtgatctt
     1441 ccgcgatgac aacccggaga ggactcatgg gaccatgacc tcgaccgttc caaactgctt
     1501 gctcggatcc tgctcaccga gcggaaagca cagttcaacc ctggtccctt gatcaggagc
     1561 cgatacgatc tcgaaggccc ccccagccaa tcggacacgc tcttccattc ccagcaaccc
     1621 gatcgtttgc ccgccttgtg cccgtcgtcg tgccagcatc acgtcgaatc cggtaccgtc
     1681 gtcttggatg acgagcgtga cccggtgatt gctccgacgc aacgtcagat cgatggtctt
     1741 cgctttggcg tacttggaga cattggtcaa ggcctcctgc gcaacccgaa aacaggccac
     1801 ctcgataggg gcggacaccc gccccgtcat ttcctcggca gagaaaaaga gagtccatcc
     1861 attgcgatcc gcctgccggt tcgcatacca ccgcagcgca gggactaacc ccaagtcatc
     1921 aaggatggac ggccgcagat tcaatgccag agtccgcact tgggtcagaa gacgatccac
     1981 cagctcaaga ccgtccgtaa gaaaatcgga acgaacctgt gccacctcgc tatgctgaac
     2041 ttcttgcaga tcgatcttca acgcggtgag caactgccca acctcatcgt gcaggtcgcg
     2101 tgcgagtcgg cttcgctcct cttcctgcac ttgcatcagc cgactggaca gcttctgcaa
     2161 agcgtggttg gcactgatca attcggcggt tcgttcctgc accttcactt ccagtgcttc
     2221 acgagcctgt tgcagaagtg tctgcgctcg tttccgttca ctgatatcac gaatgatttt
     2281 cgacacgcct acgaaccgac cggcagcatc tctgagcggc gacaaggtga ccgacacatc
     2341 gatgctccgc ccgtccttgg tcaaccgcac tgcgtcatcg gtacggatgc tttgccctct
     2401 ctgtactgag gcttgcaacc gggcttcgtg atcatggaga tcggccggaa tgagcgtcag
     2461 aatggaacga ccaagcatct cggcagcggt atatccgaac atacgctccg ccccggcgtt
     2521 ccacgacgtc acaattccgt cgggtgtcac actcaggatg gcatcactgc tgctctccac
     2581 gatggctccc agtcgcctgg tcgcatcgtc aagcttggcc gttttcgccc gagcgacaag
     2641 ccccgccgta atccagagta aagccacccc gacagttcga ttaaacacag ccatgtccgg
     2701 cgggatgccg ggaggggaaa gccgaaattc caatatgagg agtatcgtcg caatcgctgc
     2761 aacaatatag ggagtgttcg gacgttcgac ccagagagcc aacagtacgg cgaagatatg
     2821 gccgacccac acctcatatc ccatcggcga aactgtgtcg atgaagaagg tgacgacgaa
     2881 taccaaaacg atcagtattt gggcatattt actgttcatc actatccctg actcgacgga
     2941 ccacttggga tcgggatcga tgacagcgag gtgaggacat catactatac tctccgatga
     3001 ccgattgtct atggtactcc aatcccggga cagagagcga gaccgtgcgt gaaggatggg
     3061 atggagacga gaggcctagt tatttgactc agccgtttgc cggttcacga cccagacgcc
     3121 ggccaggatg aggccgatgc cgataatttc aatcagtccg acggattctc ctagaatcaa
     3181 ggccgataag ccaatggcgg caaccggcgt cagattgccc aagaccgacg cccgtgaggg
     3241 gccgatgcct ttgacgccga acaaccaggc ctgttgcgcc actgccgtcg caaaaaggat
     3301 gagatagccg agcgcaagcc aatcggatgt catcaccgat ccgatcccgg catgtatcat
     3361 cttttgatcc gtccacagca gcgggatctg aaggaccgtg gcgaccatca gggttgtcca
     3421 attgacggtc agtgctgaaa tccgctccat gatagtgcgg ctgccgatac tatagagcgc
     3481 ccaactcacg acgccgagaa agaccaacgt gctgcccaga agcggctgct cgccggccgc
     3541 ttgaaagcct gctacggaga ccagtccgac acccgcgaac gacagcaggg cgccgaccca
     3601 cactgcgcgc aacggcacat cccgaatcaa caaggctgag agcagagccg tcacgacggg
     3661 gctcgacccg atgatcacgc cgccgatcgc cccactgaca taattgagcc ccatgagaat
     3721 gagaagatgg tttcccaaca ccccaagccc aagaaacccc agcaacccca tatcggcgcg
     3781 actgattttg atcgcaccgc cttcttgcga ccaccaggtt gctaggagga tcgccagccc
     3841 tccgagatcg cgcaggaccg acgcttcgac agcggagaac gagccgaggg ccatcttc
//
LOCUS       DATMTI010001008         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_505060, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001008 DATMTI010000000
VERSION     DATMTI010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505060"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VN039_15955"
     CDS             <1..196
                     /locus_tag="VN039_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47490.1"
                     /translation="ADLTGVKLGEIIEAVLSNPQRLEKMQRKSLEMRRIDAGEVIVDE
                     CYALMGVTHDINQPVRATGG"
     gene            258..1649
                     /gene="murC"
                     /locus_tag="VN039_15960"
     CDS             258..1649
                     /gene="murC"
                     /locus_tag="VN039_15960"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWV47491.1"
                     /translation="MTLFRKTQQIHLVGIGGAGMSGIAEVLLTMGYKVTGSDLHASET
                     TKRLEELGGKVFVGHQESNVGEAQVVVISSAVAASNPEVVAAKAKQIPVIPRAEMLAE
                     LMRLKFGVAIAGAHGKTTTTSMVANVLAQGGLDPTMVIGGKVNALGSHARLGRGDLLV
                     AEADESDGSFLRLSPTIVAVTNLDREHLDHYGSMERINESFLEFINKIPFYGLAVLCG
                     DDDRLTALFPRLVKRYHTYGLRDRGGAAPDFKATDISLRQWGAEFRTYFRGRNLGPFR
                     LAVPGIHNVSNALAAIAIGFELEIPVDLIRKGLAAFTGVERRFHLRGEAGGIMVVDDY
                     GHHPTEVKATLTAAKQGWDRRLVVLFQPHRYSRTRDCIGEFAHAFDYADLLFTTEIYP
                     AGESPIPGVSGSALAETIKSTGHPSVSFIERKETLPDQVLPHLRPGDLVLTLGAGDIW
                     KAGTGILARLESA"
     gene            1649..2599
                     /gene="murB"
                     /locus_tag="VN039_15965"
     CDS             1649..2599
                     /gene="murB"
                     /locus_tag="VN039_15965"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWV47492.1"
                     /translation="MVQKHRVQTDGTKVRPLRVQHRLESAVAGVRGLVRFNAPLKEYT
                     SFHIGGPADVLVEPADVEDIVRLTKQTREQKIPIFVLGGTNVLIRDKGIRGVVVDLAK
                     LRAIKEEPGSVLYAEGGVGMPTLIGQAIRRSLAGLEWAAGIPGTVAGCVVMNAGTRLG
                     EMKDSVKAVRIVSPKGALIDCPAEAIEFRYRRATLPPGVVVGVWLQLRAGVRSDIEKV
                     VKDYLHYRRDTQPLTLPSAGCVFKNPLNDSAGRVVEAAGLKGTSVGDAQVSIKHANFI
                     VNQGHASAADVLSLIEKVRTRIAQETGIKLELELKIVGEV"
BASE COUNT          589 a          642 c          853 g          570 t
ORIGIN      
        1 ggcagatctt accggagtga aactgggtga aataatcgaa gctgttttat cgaacccgca
       61 gcgactggag aagatgcaac ggaagagttt ggagatgaga cgaatcgatg ccggcgaagt
      121 gatcgtcgat gaatgttacg cgctgatggg agtgacgcat gacatcaacc aacctgttag
      181 agcgaccgga ggttaaggtt gttggggaac gggagattca atcgcagact tctgcgggac
      241 gacaaaggct aaaccgtatg acgctctttc gcaagacaca gcagattcat ctcgtcggaa
      301 tcggcggagc cggtatgagc ggtatcgcag aagttctact cacgatggga tacaaagtga
      361 ccggttcaga cttgcacgct tcggagacga cgaagcgact ggaagaactc ggtgggaaag
      421 tgttcgtcgg tcatcaggag tccaatgtgg gggaggcgca agttgtggtc atctcttccg
      481 ccgtggcggc gagtaaccct gaagttgtag ccgcgaaggc gaagcagatt ccggtgattc
      541 cgcgggcgga gatgttggcg gaattgatgc gtctgaagtt cggggtggca atagccggcg
      601 ctcatggaaa aaccaccaca acgtcaatgg tcgcgaacgt gttggcgcag ggtggccttg
      661 accccacgat ggtcattgga ggcaaggtca acgccttggg cagtcacgca cggcttggac
      721 gaggcgactt gttggtggcg gaggcagacg aaagcgatgg atcgtttctt cggctctccc
      781 cgaccatcgt ggcggtgacc aatctggacc gcgagcatct tgaccactac gggtcgatgg
      841 aacgcatcaa cgaaagcttc ttggagttca tcaataaaat ccccttttat gggttggcgg
      901 tactgtgcgg cgatgatgat cgtctgaccg cactgttccc tcgcttggtc aaacggtatc
      961 acacctatgg gcttcgcgat cggggcgggg cggcaccgga tttcaaggca accgacatca
     1021 gcctgagaca atggggcgcc gagttccgga cgtattttcg tggaaggaat cttggcccct
     1081 tccgattggc cgtgccgggc atccacaacg tttcaaatgc gttggcggct attgccatcg
     1141 gcttcgagct ggagattccc gtcgatctga ttcgcaaagg gttggcggcc ttcacgggag
     1201 tcgagcggcg atttcatttg cgaggcgaag ccggcggtat catggtggtc gacgactatg
     1261 gccaccatcc cactgaggtg aaagcgaccc tcactgccgc caagcagggc tgggaccgtc
     1321 gattagtggt tctttttcag ccgcatcgat acagccggac gagggattgc attggagagt
     1381 tcgcgcatgc ctttgactat gccgacctgc tgttcacgac ggaaatttat ccggctggtg
     1441 aatcaccgat accgggagtt tctggatccg ccctggctga aacgatcaag tcgacgggcc
     1501 atccatcggt gagcttcatc gagcgcaagg aaacgctgcc ggatcaggta ctccctcatc
     1561 taagaccggg cgatctcgtc ctcacgttgg gcgcgggtga tatctggaag gctggaaccg
     1621 ggattcttgc ccggcttgag tctgcttaat ggttcagaag catcgagtgc aaacggatgg
     1681 aacaaaggta cgaccattgc gggtacagca cagattggaa tccgctgtgg cgggcgttcg
     1741 cgggctggtt cgattcaatg cgccgcttaa agagtacaca tcgttccaca tcggtggtcc
     1801 ggccgatgtg ttggtggagc cggccgatgt ggaagatatt gttcgtctta cgaagcagac
     1861 gcgcgaacag aaaattccga tcttcgtgtt gggcggcacc aacgtcctca ttcgtgacaa
     1921 agggattcga ggtgtggtgg tcgatctggc aaaattacgc gcgatcaaag aggaaccagg
     1981 atccgtgttg tatgccgaag gcggggtcgg catgccgacg ctgatcggcc aggctattcg
     2041 tcggtctttg gccggattgg agtgggcggc cggtattccc ggcactgttg caggctgtgt
     2101 cgtgatgaat gcagggacac gactcggtga aatgaaggac tcggtgaaag ccgtccggat
     2161 cgtgtcgcca aaaggcgcac tgattgactg cccggcagaa gcaatcgaat ttcgatatcg
     2221 tcgggcgacg ttgccgccgg gtgttgtcgt cggagtatgg ctgcagctga gggcgggagt
     2281 gcggtcggac atcgaaaagg tcgtgaaaga ctatctccat taccgccgtg atacgcagcc
     2341 cctcacgcta ccgagcgccg gctgtgtgtt caagaatcca ctgaatgatt cggcagggcg
     2401 ggtcgtcgag gcggcaggac tcaaaggaac atcggtcggt gatgcacagg tttcaatcaa
     2461 gcatgccaat tttatcgtga atcaagggca tgccagcgcc gccgacgttc tttcgttgat
     2521 tgagaaggtg cgcactcgaa tcgctcaaga gacggggatc aaattggagc tggaattaaa
     2581 aatcgtgggt gaggtgtagc gaaaggaaat agtcgtggcg attgacctta tgacacatgc
     2641 tcggatcggt gtac
//
LOCUS       DATMTI010001009         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_462578, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001009 DATMTI010000000
VERSION     DATMTI010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462578"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VN039_15970"
     CDS             complement(<1..362)
                     /locus_tag="VN039_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47493.1"
                     /translation="MQSYTANLMGNRDPYYSSKYELTLPTHLAIIAMTRSIRHFLKKR
                     SSFVAAFIMPPKADVHAYKMAAKWLLEGTVLDSGEHGNNYVLVVDEETDLADDFEAFR
                     KLRELKRAIILLQDGTLLS"
     gene            complement(409..1026)
                     /locus_tag="VN039_15975"
     CDS             complement(409..1026)
                     /locus_tag="VN039_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47494.1"
                     /translation="MGIAQRARSVRASRHSASIRTFAPAATIKCDGPSIFRSQVARDT
                     ACLLDVNPAVVSWHCMPVSLDCGNVAHVPDFGIVDDDGGRMLIDSPDRVLPIDAEAIA
                     AVAETAGTRYRRLLADEVYEGARLRNTKDLLRYGGTVLALGDRLRLLAVLEEHGNLTI
                     AECLTVFVETKPVAGLAQMILRGFVEVDLDTELLGPETMVRRIRP"
     gene            complement(1191..2291)
                     /locus_tag="VN039_15980"
     CDS             complement(1191..2291)
                     /locus_tag="VN039_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWV47495.1"
                     /translation="MTSILTNVSAITALNTLRGIGAQLTDTSQQMSSGLRVDIASDDA
                     AYWAISTSMRSDVGAMEAVTDSLGLALGIVDTAYAAMETVRQSFVEIRNLAITASDMP
                     QPGMQDAIKPTFSLDPEYAKSDVYKIDVRIQQLQDQARAAMLSASFAGVNMIYHGKNE
                     AVDASQQVFSFVIGYAAGKVQTMDVSAMDTLLLNDDFGSYPTTYPGEFNPEKTLFDGS
                     DIVDAPGSFTPAAIYWYNIPVSNPATGDPESYPVNPTYILQSIENHVVRFGSDRQGLY
                     SNLVADIDGKIQQLTSKMAYVGSVQSSLGLYEENNKKRIDAATRGIGRLVDADMEVTA
                     VKLRALETQQQLATNGLQIANSQPSTLLQLFR"
BASE COUNT          551 a          684 c          687 g          547 t
ORIGIN      
        1 gacaaaaggg taccatcctg taggagaatg atagctcgct tgagttctcg cagtttcctg
       61 aaggcctcga aatcgtcggc aagatcagtc tcctcgtcaa cgacgagaac gtagttgtta
      121 ccgtgttcgc cactgtccag caccgtcccc tcaagcaacc actttgcggc catcttgtaa
      181 gcatgaacat ccgctttcgg cggcatgatg aaagcggcca cgaaagatga tcgctttttc
      241 aaaaaatggc ggatggatcg cgtcattgcg atgatcgcaa gatgggtcgg cagggttagc
      301 tcgtatttcg agctgtagta cggatcgcgg ttgcccataa gattggctgt ataggactgc
      361 attggatacc tcggaactcg gagaataatc gtgacgctga cgggacagtc agggccgtat
      421 tctccgcacc atggtctcag ggccgagtaa ttccgtatcg agatcgactt cgacgaaacc
      481 gcgcaagatc atctgggcca atccggcaac tggcttagtt tcgacgaaaa ctgtcaggca
      541 ttcggcgatc gtcaggttac catgctcttc gaggacagcc agcaatcgta aacgatcacc
      601 gagcgccaga acggtgccgc cgtacctaag caaatccttc gtattgcgaa gccgtgcacc
      661 ctcgtagact tcgtcggcaa gcagacggcg ataacgcgtg cccgctgtct cagcgacggc
      721 cgcaattgcc tcggcgtcga taggcaacac cctatccgga ctgtcgatga gcatccttcc
      781 accatcatca tccacgatgc cgaaatccgg gacatgagct acgttgccac aatccaggga
      841 aacaggcatg cagtgccacg aaacgacggc aggattgaca tccagcaggc atgcggtgtc
      901 acgcgccacc tgggagcgaa aaatagatgg tccgtcgcat ttgatcgtgg cggcgggagc
      961 aaatgtgcgg atggacgctg aatgacggct ggcgcgaacc gatctcgcgc gctgtgcgat
     1021 acccatattg aaaacctcag actaaacatg gccgatcgtc gccgcgactg cgcagcgaaa
     1081 cgaaatcaac ctattcgtat gaggtgggtc atcttttgca tgcgagaaca attagtgaac
     1141 aaatatccaa aggtcaagat atccctgtag attttattgc gtcgaaattg ctatcgaaaa
     1201 agctgtagaa gtgtggacgg ctgggaattg gcgatttgca atccattggt cgccagttgc
     1261 tgctgggtct ccagcgccct cagcttgacg gccgtcactt ccatgtcagc atccacaagc
     1321 cttccgatgc cccgtgtcgc ggcgtcgatc cgcttcttgt tattctcctc gtacaagcca
     1381 agcgacgact gcaccgaccc cacgtaggcc atcttggagg tcaactgctg gattttgccg
     1441 tcgatgtcgg cgacgaggtt gctgtagagg ccctgcctgt ccgacccgaa acggacgacg
     1501 tggttctcga tcgactggag gatataggtg ggattgaccg ggtaggattc aggatcgccc
     1561 gtagcggggt tcgataccgg gatgttgtac cagtagatcg cggcgggagt gaacgatccc
     1621 ggcgcgtcga cgatatcact gccgtcgaac agcgtctttt ccgggttgaa ctcacctgga
     1681 tacgtcgtcg ggtagctgcc gaagtcgtca ttcagaagca gggtgtccat ggcgctgaca
     1741 tccatcgtct gcaccttgcc ggccgcgtat ccgataacga aggagaatac ctgctggctg
     1801 gcgtcaacgg cttcgttctt gccgtggtaa atcatgttca cgcccgcgaa cgacgcagac
     1861 agcatcgccg ctcgcgcctg gtcctgaagc tgctgtatcc gcacatcgat cttgtagacg
     1921 tccgatttgg cgtattctgg atcgaggctg aaagttggct tgatcgcgtc ctgcatgccg
     1981 ggctggggca tgtcgcttgc ggttatggca aggttcctga tctcgacgaa tgactgcctc
     2041 acggtttcca tcgccgcata tgcagtatcg acgatcccca acgccagccc gagactgtcg
     2101 gtgacggcct ccatcgcacc cacgtcagac cgcatcgatg tcgagatggc ccagtaagct
     2161 gcatcgtcag aggcgatgtc gaccctaagg ccggacgaca tctgctgcga tgtgtctgtg
     2221 agctgcgcgc cgatcccgcg cagcgtattc agcgccgtta ttgccgaaac gttcgtcagg
     2281 atgctggtca ttcaatatcc gtatttgcaa tctggaacag tgaagccaca tgtcagcttc
     2341 aacgacagat ttcggatatc ggcgttaaca accgcggcaa attaggaggg tagaaatacc
     2401 ctctcgcgaa gatttcccga gcacttcctc catttcacca ggtcggtagc tttcaatttg
     2461 gttgctaca
//
LOCUS       DATMTI010001010         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_292034, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001010 DATMTI010000000
VERSION     DATMTI010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292034"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /gene="ispF"
                     /locus_tag="VN039_15985"
     CDS             complement(<1..373)
                     /gene="ispF"
                     /locus_tag="VN039_15985"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWV47496.1"
                     /translation="MKKRSRIGYGYDVHPLGADRPLILGGIEIPHIKGLLGHSDSDVL
                     VHAVCDALLGAMGEGDLGRHYPSSDPKYKGISSLKLLEDVMAKLKVKRYRVGNIDSVI
                     VAQAPRLGPHLPAMQKKIAETA"
     gene            complement(370..1089)
                     /gene="ispD"
                     /locus_tag="VN039_15990"
     CDS             complement(370..1089)
                     /gene="ispD"
                     /locus_tag="VN039_15990"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWV47497.1"
                     /translation="MNNHTVALVPAAGRGLRMGGSVPKQFLALGGQPLILHSLRVLQA
                     SSVIEEIILAVPQNEMDYSLKEIVAKHRFTKVTKVVPGGHERQDSVRHALEAVHDDVD
                     VVLVHDAVRPFLTGHMVEEVVKTARAKGAAIIALPMKDTVKQVGADHVIERTVDRQSL
                     WLAQTPQAFRRDWLLAAHRKAHAEGIRATDDASLIEWAGHPVCVVEGTGENIKVTRPE
                     DMVIGEAILASRQSRGMKGSV"
     gene            complement(1191..>2303)
                     /locus_tag="VN039_15995"
     CDS             complement(1191..>2303)
                     /locus_tag="VN039_15995"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWV47498.1"
                     /translation="LIITNNHVVGKADEIRVTLSDKREFKAKLIGTDPKTDVAVVKID
                     ATGLPTVTWADSDKLEVGEFVLAVGNPFGLTQTVTLGIVSALGRAAGIAEYEDFIQTD
                     AAINPGNSGGPLVNVRGELVGMNTAIFSQSGGNMGIGFAVPSNMAQSVMNQLVQTGKV
                     VRGWLGVSIQELTPELASQFGITETKGILVSDVMDDSPAKKAGFERADVIVEYDGRSM
                     DSPTHLRNAVAQTPVGKKVTIKFIRDKKPKTVDLTIVEQPKSMSQNGEDDGGESATPT
                     GVLSSLDVRDLTQELASRYGLKSSERGVVIVRVKPGSTAEELGVREGDVILEVNRQTV
                     TSVKVFERIAGKLPKDQAVLLLLKRQGRTIYLTLRP"
BASE COUNT          481 a          719 c          573 g          530 t
ORIGIN      
        1 cggcggtctc cgctattttc ttctgcatcg ccggaagatg cgggccaagc cgaggggcct
       61 gagccacaat cacggagtcg atgtttccca cccgatacct cttcactttc agcttcgcca
      121 taacgtcttc cagcagtttc aagctggaaa tccccttata cttggggtcg gaacttggat
      181 agtgcctgcc gagatcccct tcccccatcg cccccaataa agcatcgcat acggcatgaa
      241 caaggacgtc ggaatcagaa tggccgagca atccttttat gtgtggaatc tcaatccctc
      301 ccaagatcaa tggacggtcc gccccgagcg ggtgaacatc gtagccatag ccgatgcgag
      361 acctcttctt catactgatc ctttcatgcc ccgagattgc cgcgatgcca agatcgcttc
      421 gccgatcacc atgtcttccg gcctcgtgac cttgatgttt tcgccggttc cttcgacgac
      481 acacaccgga tgtccggccc actcgatgag agaggcatca tccgttgcac gaatcccttc
      541 tgcgtgggcc ttcctgtggg ctgccaacaa ccaatcgcga cggaaggctt gcggggtttg
      601 agccaaccac aatgactgcc gatcaaccgt gcgctcaatg acatgatcgg ctcctacctg
      661 cttgacggta tccttcatgg gaagggcgat aatcgccgct cccttggccc gtgccgtctt
      721 cacaacctct tccaccatat gccctgtgag aaacggacgc accgcatcat gtaccaacac
      781 cacatcgaca tcgtcatgga ccgcctcaag cgcatgcctg acagaatctt gccgttcgtg
      841 tcctcccggc accactttag tgaccttggt aaagcgatgc ttcgccacga tctctttgag
      901 actataatcc atctcgttct gaggaacagc cagaatgatt tcctcgatga cggacgaagc
      961 ctgaagcaca cgaagagaat ggaggatgag aggctgaccg ccgagtgcaa ggaattgttt
     1021 gggcaccgag ccgcccatgc gaaggcctcg cccggcggca gggacaagag ccacggtgtg
     1081 gttattcacg actggagtct agactatcct catcgcggga agcgtacgaa cagatggtgc
     1141 ctgcgcaacc tcgacaccaa catcccatat ctgatcacct cggcctgtcc tcatggacga
     1201 agcgtcaggt agatcgtccg cccctgccgt ttcaacaaga gcaagaccgc ctgatccttc
     1261 ggcaacttac cggcgatccg ctcaaaaacc ttcaccgacg tcacagtttg acgattcact
     1321 tcgaggatga catcgccctc tcgaacacct aattcttcag ccgtactccc gggcttgacc
     1381 cgaacgatca ccacgccccg ctcacttgac ttcagtccgt accggctcgc caattcttgg
     1441 gtcaagtctc ggacatcgag gctggagagc acgccggtcg gcgtggctga ttcccctcca
     1501 tcgtcttcac cgttttgcga catcgacttg ggctgctcga cgatggtgag gtcgaccgtt
     1561 ttcggttttt tatcgcgaat gaacttgatg gtgacttttt ttccaaccgg cgtttgagcc
     1621 actgcattgc gcaagtgagt cggtgaatcc atggatctgc cgtcatactc gacgatgacg
     1681 tcggcccgtt cgaatccggc ctttttcgcc gggctgtcgt ccatgacatc gctcacgagt
     1741 ataccctttg tctccgtgat accaaattgg gatgccaatt ccggcgtcaa ctcctgaata
     1801 gatactccaa gccagccgcg gacgactttt cccgtttgta cgagctggtt catcacggac
     1861 tgagccatat tgctcggcac ggcaaaaccg atccccatgt tgcctccgct ctgactgaat
     1921 atagcggtat tcatccctac caattcgccc cgcacattga ccaagggtcc accggagttg
     1981 cccgggttaa tggccgcatc cgtctggatg aaatcttcgt actcggcaat acctgcggct
     2041 cgccctaggg cactgacgat ccccaaggtg acggtttggg ttagcccgaa tggattcccg
     2101 acggccagga caaactctcc gacttccagt ttgtccgaat cggcccaggt tacggtcgga
     2161 agccccgtcg cgtcgatctt cacgacggcc acatcggtct ttggatcggt gccgatcagc
     2221 ttcgctttga attcgcgctt atccgacagg gtgacacgaa tttcatccgc cttgccgacc
     2281 acatgattgt tggtgatgat gag
//
LOCUS       DATMTI010001011         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_547902, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001011 DATMTI010000000
VERSION     DATMTI010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547902"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VN039_16000"
     CDS             complement(<1..934)
                     /locus_tag="VN039_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV47499.1"
                     /translation="MATAFDVIVVGGGHAGCEAALAAARMGAKTLLLTMELGRIAQMS
                     CNPAIGGIAKGHLVKEIDALGGEMGRNTDRSGIQFRFINTSKGPAVRALRAQCDKSLY
                     RMAMQESLTSQKHLTLAEGVVDRLLVAGGAVTGIMTGSGERIDGKAIILTSGTFLKGL
                     IHIGLSHFPAGRAGEASAEHLSDCMRDLGFEIGRLKTGTPPRLDKATIDFSVMIPQPG
                     DSPPPPFSYRTQHITTRQVLCHLTYTSRETHDIIQGNLDRSPLYSGIIESTGPRYCPS
                     IEDKVVRFAEKERHQIFIEPEGLNSTEFYPNGIST"
     gene            complement(943..2394)
                     /gene="mnmE"
                     /locus_tag="VN039_16005"
     CDS             complement(943..2394)
                     /gene="mnmE"
                     /locus_tag="VN039_16005"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HWV47500.1"
                     /translation="MSAVGTPEDTICAIATPAGEGGIGIVRISGQHAVDIGSRVARLR
                     SRQSFHTLASHKLHLADILFPSVPSIDNLPDSETGQSTDQMIDEGLVVYMKAPRSFTA
                     EDVVEIHCHGSGLVLQRVCEACVAAGARLAQAGEFTKRAFLNGRLDLSQAEAVLDTIR
                     AKSELGLKLAQRQLRGELAQQVHRLRAGLVGLLAHLEAGIDFAEEDIAFVRRDEMIVS
                     LQETLSHVQRMLTTAEAGRVLRDGVRVVIAGEPNVGKSSLLNSLLRENRAIVTDIPGT
                     TRDIIEESVVWLGLRVTLTDTAGMRDTTDLVEREGIRRSKEAQDQADIVLHVLDGSQL
                     TTSDLGNVCLPSVAHEHLLVVNKIDLVDAASVQSLSQALRARRPCKVLPISVRTGEGL
                     EALKVAIRAQFVKPSFEASDGVVVTHVRHRVALERAETSLHESLVSIERGLEPEFVAV
                     DLRGAADALGEIVGAITSDEVLDRIFSEFCIGK"
     gene            complement(2446..>2641)
                     /locus_tag="VN039_16010"
     CDS             complement(2446..>2641)
                     /locus_tag="VN039_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HWV47501.1"
                     /translation="PTQAKVMLMLPALMTFLFINFPAGLVLYWLTNNVLTIVQQFVTD
                     RFFFRTPAIAPAMDEQVGKK"
BASE COUNT          611 a          755 c          708 g          567 t
ORIGIN      
        1 tggttgaaat accgttcggg taaaattctg ttgagttgag gccctcaggt tcaataaaaa
       61 tctgatgccg ttccttctcg gcaaaacgta cgaccttatc ttcgatggaa gggcagtacc
      121 gaggcccggt agattcgatg attccgctgt acagaggcga gcggtcgaga ttcccttgaa
      181 tgatgtcgtg tgtctcccgg ctggtatagg tcaagtgaca gagaacttgt cgggtcgtga
      241 tgtgttgagt gcgatacgaa aaagggggag gaggactatc gccgggctga gggatcatca
      301 ccgaaaaatc gatcgtagcc ttgtccaatc ggggaggagt tcccgtcttg agtcgtccga
      361 tctcaaatcc gaggtcccgc atgcaatctg aaaggtgttc agccgaggcc tctcccgcac
      421 ggccggccgg gaaatggctg agcccaatat ggataagacc cttaaggaaa gtgcccgatg
      481 tcaatataat tgccttgcca tcgatccgtt cacccgagcc tgtcatgatc ccggttaccg
      541 cacctcccgc cacgagcagt cgatccacga ctccttccgc tagggtaagg tgtttctgcg
      601 aggtgaggct ttcctgcatg gccatgcgat acagcgattt atcgcattgg gcgcgcaacg
      661 cccgcaccgc cggtcctttg cttgtgttga tgaatcgaaa ttgaatcccg gaacgatccg
      721 tattccgccc catctctccg ccgagcgcat cgatctcctt cacgagatgc cccttggcga
      781 tcccgccaat tgccggattg cacgacatct gtgcgattcg tcccagttcc attgtcaaaa
      841 gcaaagtttt cgcgcccatt cgtgcggcgg caagtgcggc ttcgcagccg gcatgaccgc
      901 cccccaccac gatgacgtca aatgctgtgg ccaccggttc ctctatttac cgatacaaaa
      961 ctcggaaaaa atgcggtcca acacttcatc ggatgtaatt gccccgacaa tttcgcccag
     1021 cgcatcggct gcacctcgga gatcgaccgc cacaaactcg ggctctaggc ctcgttcgat
     1081 cgagaccagt gactcatgga gtgatgtctc ggcccgttcg agcgccacgc gatgtcgcac
     1141 atgagtgaca acgacaccat cgctggcttc aaaggacggc tttacaaact gtgctcgaat
     1201 agcgaccttc aatgcctcca atccctcacc cgtacgaacc gagatgggaa gaaccttgca
     1261 cggtctacga gctcgaagcg cttgagagag cgattgtacc gatgcagcgt cgactaaatc
     1321 gattttattg acgacaagca aatgttcgtg agcgactgaa ggcaaacaga cattccctag
     1381 gtcgctcgtt gtcagttgag agccatccaa gacatgcagg acgatatctg cctggtcctg
     1441 agcttccttg gaccggcgga taccttctcg ttcaacgagg tccgttgtat cacgcatccc
     1501 agcggtgtcg gtcaaggtca ctcgcagacc aagccagacg accgattctt cgataatgtc
     1561 gcgagtcgtg cccgggatgt cggtcacaat agctcggttc tcacggagta aactattcag
     1621 caaacttgac ttaccgacat tgggctctcc ggcgatcacc acccggacgc cgtcgcgcag
     1681 tacgcgtccg gcctcagcag tcgtcaacat ccgctggaca tgggacagcg tctcctgcag
     1741 ggagacaatc atctcatcac gtctaacgaa cgcaatatct tcctcggcga aatcgattcc
     1801 tgcttccaag tgggctaaca ggcctacgag accggcgcgc aatcggtgga cctgctgtgc
     1861 gagttcgccg cggagctgac gttgtgccaa tttaaggccg agttcggact tggccctgat
     1921 ggtgtccaac acagcctcag cctgagacaa atccaggcgg ccgttcaaga aggcccgttt
     1981 ggtaaactct cctgcctgtg cgagacgagc tccggcagcg acgcaggcct cacagactcg
     2041 ttgtagaacc aacccacttc cgtggcaatg aatctcaacc acgtcctccg cggtaaaaga
     2101 tcgaggtgcc ttcatataga ccaccagacc ttcgtcgatc atctggtcgg tcgattgacc
     2161 tgtctcggag tcgggcaggt tatcgattga aggaacagac ggaaagagaa tatcggccag
     2221 gtgcaattta tgagaagcaa gcgtatggaa cgattgcctg gaacgcagcc gggcaacgcg
     2281 gctccctatg tctacggcat gctgcccgct tatccgaacg ataccgatac ctccctcacc
     2341 ggcaggagtg gcaatcgcac aaatggtatc ttcgggtgtt ccaacggccg acatgttcac
     2401 acacattcgg agggacgact cacatcgagc gaaaccgacc gagcatcatt tcttgccaac
     2461 ctgttcgtcc atagccggtg ctattgcggg ggtccgaaag aaaaagcgat cggtgacgaa
     2521 ctgttgtacg atggtgagca cgttattggt caaccaatac agcaccaggc cggcaggaaa
     2581 attgatgaac aggaacgtca tgagtgccgg aagcatcagc atgactttcg cctgagtcgg
     2641 g
//
LOCUS       DATMTI010001012         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_845427, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001012 DATMTI010000000
VERSION     DATMTI010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845427"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     misc_feature    complement(152..1531)
                     /note="23S ribosomal RNA rRNA prediction is too short"
BASE COUNT          343 a          628 c          448 g          430 t
ORIGIN      
        1 gtctcggcag tagagcgccc atacttgcat ctcgtccgca gaaccgatcg ccatcaccac
       61 aaaccggcga aactccggct ttgacaggcg ctgccgtcca aagccctccg ccagattggc
      121 gcagatcgat ttcgatgccc ggcgaacctg atccgccaag gcaaactgct cgatccgcgg
      181 gaagtcgagg ctcgcccgat gcacttccag cgatatccga taggctcgct gaaacacatc
      241 gagatcttcg cctcgctcga tccattgctc gttcatcaat tctgccgttc cgacttctga
      301 tttctgcctt ccggaatccg cagcttcggt gcatggcttt agccccgtta cattttcggc
      361 gcaggacggc ttgaccagtg agctattacg ctttctttaa aggatggctg cttctaagcc
      421 aacctcctgg ttgtcttggc cgtcctacat cctttcccac ttagccacga ctgggggacc
      481 ttagctggcg gtctgggctg tttccctctc gacgacggac cttagcaccc gccgtctggc
      541 tgccgcgctg cactcgccgg tattcggagt ttggttaggt ttggtaaggc tcgcgccccc
      601 ctagcccatc cagtgctcta cccccggcgg tgagcgcctg acgctctacc tcaatagatt
      661 tcgcggagaa ccagctattt ccgggtttga ttggcctttc acccctagcc acaggtcatc
      721 cccgactttt gcaacagtcg tgggttcggt cctccagtgc gtgttaccgc accttcaacc
      781 tgcccatggc tagatcaccc ggtttcgggt ctgatcccac taactaaagc gcccagttaa
      841 gactcgcttt cgctgcgcct gcgcctatcg gcttaagctc gctagtaaga ccaactcgct
      901 gacccattat acaaaaggta cgccgtcagc cagggatcag gcatcaggaa tcagtcacca
      961 gaatttctga tccctgatcc ctgattactg atccccgaac ctccgactgc ttgtaggcat
     1021 ccggtttcag gaactgtttc accccgctca tcgcggtgct tttcaccttt ccctcacggt
     1081 actggttcac tatcggtcac tgaggagtac ttaggcttgg agggtggtcc ccccatcttc
     1141 agacaggatg gctcgtgtcc cgccctactc gaggactaag gctccggttt cccctacggg
     1201 gctgtcaccc gctccggccc gcctttccag acggttcagg taccaaaagc tttagccact
     1261 ggcctggtcc gcgttcgctc gccactacta gcggagtctc ggttgatgtc ctttcctccg
     1321 gctactgaga tgtttcagtt cgccgggttc gcctcccaga cctatggatt cagcctggga
     1381 taccccccaa gttgccctgg gcgggtgggt tgccccattc ggagatcccg ggatcaacga
     1441 tcgctcgcat ctcccccggg cttttcgcag cgtgccacgt ccttcatcgc ctctcagtgc
     1501 caaggcatcc accagatgcc cttcctttgc gcttgagcac cgcacccaac ggcaaagatc
     1561 atccagatat cagaacacag atatcagatg tcagaacctt gaggcggcaa actctccttc
     1621 tcagaagaca cttcgcgcgg aaccaaccaa aacggccttc cggccgccgc ggtcgttccg
     1681 ccacggtcac acaactcgct tctttacgat tgtccatcaa caaacccgca gccctcccta
     1741 acgaggagcc gccggcaatc tcttcttccc gccgcgtcgc aacacaggtt ccgatccctg
     1801 tctccctgat gcccgacaaa gtggaggtga tcgggctcga accgacgac
//
LOCUS       DATMTI010001013         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_759208, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001013 DATMTI010000000
VERSION     DATMTI010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759208"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VN039_16015"
     CDS             complement(<1..887)
                     /locus_tag="VN039_16015"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWV47502.1"
                     /translation="MPRALIIGDAGQDGQILWDQLVARGFSLLGISRRAIRTHSTQWS
                     GDVAITVLQDVQDMMAQFRPDYVYYLAAHHHSSQDPGGEDASAWEDSWAIHVHAFSNV
                     LHAARECCSGVRIFYASSSRVFGEASASPQDEQTLLAPRCVYGVTKASGMMLADYFER
                     THGLFVSCGILFNHESPLRGSQFVSQRIADGLVAIKYGQESVLEIGSLDARVDWGYAP
                     DYTRAMQMVLECGSPGHFVIASGQNHSVREMVDAAAERLGLSGETRIVETANILRRRS
                     QDLCGNPSRLRACTGWKQKV"
     gene            complement(887..2149)
                     /locus_tag="VN039_16020"
     CDS             complement(887..2149)
                     /locus_tag="VN039_16020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV47503.1"
                     /translation="MITSSTVDGIKEISKCRISGSTHLVSVLNLGQQSLTGVFPRDPT
                     QEVTCGPLELVWCPDSGLLQLKHSYSLSEMYGDNYGYRSGLNSSMVAHLNGKVRGLER
                     QNRLVSGDWVLDIGSNDGTLLKAYATPDIRRVGMDPTAGKFRSFYPDEVEVVTDFFDG
                     ERFLGVSGGHRAKIITSIAMFYDLEDPNAFVHDISCALAPDGMWHFEQSYMPSMLRTN
                     AYDTVCHEHLEFYSLRVIRNLLARHGLRIVDVQMNSVNGGSIAVTACHEKADIPSARP
                     IIDWMLQQEDNWGLNSVKPYRQFEDRVFKHRADLQRLIEVLVADGKLVLGYGASTKGN
                     VLLQFCQFGPRQIPAIAEVNLEKFGTYTPGTLIPIISEEEARAMRPDYFLVLPWHFKS
                     TIVQREKEYLASGGKLIFPLPEIEIVGD"
BASE COUNT          583 a          661 c          571 g          530 t
ORIGIN      
        1 accttctgct tccaacccgt gcatgctcgc aagcgagacg gatttccaca cagatcctgc
       61 gatcgacgcc gaagaatatt agccgtctca actattcgtg tttcacctga gagcccaagg
      121 cgctctgcgg ctgcgtctac catttcgcga acgctatgat tctgaccgga agcgatgaca
      181 aaatgtccag ggctgccgca ttcaagaacc atttgcatcg cgcgtgtgta atcaggcgcg
      241 taaccccaat ccacacgcgc atcaagacta ccgatttcca atacactttc ttggccgtac
      301 ttaatcgcaa ctaaaccatc ggcgatacgt tgggaaacaa actgcgaacc gcgcagtggc
      361 gattcgtgat tgaacaaaat tccacaggac acaaacaatc cgtgcgtccg ctcgaagtaa
      421 tcggccaaca tcatccccga ggcctttgtg acgccataga cgcagcgagg agcaagcagt
      481 gtttgttcgt cctgtggact agcgctagct tcaccgaaca ctcgagacga tgatgcataa
      541 aatatccgca ccccggaaca gcactctctg gcggcatgga ggacattaga aaaagcgtgt
      601 acatggatgg cccagctgtc ttcccacgcg ctggcgtcct cgcccccggg gtcctgcgag
      661 ctgtgatggt gtgctgccaa ataataaacg tagtccggtc tgaactgggc catcatgtcc
      721 tgcacgtctt gcaggaccgt gatagcgaca tctccgctcc actgcgtcga gtgcgtgcgt
      781 atcgcccgtc gtgaaattcc aagcagggaa aaccctcgtg cgaccaattg gtcccacaaa
      841 atttgcccgt cctggcccgc atccccaatg atcaaagcac gaggcatcaa tcacctacta
      901 tttcgatttc cggtagaggg aaaatgagtt tcccgccaga ggcgagatat tccttttccc
      961 gctgcacgat cgtggacttg aagtgccatg gaagaaccag aaagtaatcc ggccgcatgg
     1021 cacgcgcctc ctcttcagag atgatcggga taagagttcc cggtgtatag gtgccgaact
     1081 tctccagatt tacttcggcg atggctggaa tctgccgagg accaaactgg cagaactgca
     1141 aaagaacgtt gcctttggtt gacgcgccat agccaagaac aagcttgcca tcggctacaa
     1201 gaacctcgat caaccgctgc agatctgcgc ggtgtttgaa tacccgatcc tcgaattgcc
     1261 ggtaaggctt gaccgaatta aggccccagt tgtcctcttg ctgcaacatc cagtcgatga
     1321 tgggtcttgc actgggaata tcagcctttt catggcaggc ggtgaccgca atgctgccgc
     1381 cattgacact attcatctgc acgtcaacga tgcgaaggcc atgtctcgct aaaagatttc
     1441 gtattactcg aagtgaataa aactcaagat gttcatggca gaccgtatca taggcatttg
     1501 tacgcaacat cgacggcatg tagctctgct cgaaatgcca catcccatct ggcgccagcg
     1561 cacatgaaat atcgtgaaca aaagcgttcg ggtcttccag gtcgtagaac atggcgatcg
     1621 acgtaatgat ttttgcgcga tgccctcccg agacgccaag gaagcgttct ccgtcgaaga
     1681 aatccgtcac aacctcgact tcatcgggat agaagctccg aaatttcccc gctgtcggat
     1741 ccatccctac gcgacggata tcaggagttg cgtaggcctt gagcaaggtt ccatcattgc
     1801 tgccaatgtc cagtacccag tctcccgata ccagccgatt ctgccgttcc agtccccgca
     1861 ccttgccatt caggtgcgca accatcgatg aattcaatcc ggatcgataa ccataattat
     1921 ccccatacat ttcggagagg ctatacgagt gcttgagctg cagtaatcca ctgtccgggc
     1981 accagaccaa ctcgagtggc ccgcaagtaa cttcttgcgt cggatctcgg ggaaacacac
     2041 cagtcaatga ttgctggcca aggttgagaa cggatacaag atgcgtgctt ccgctgattc
     2101 ggcatttgct gatttccttg atgccgtcaa ctgtggaact tgtaatcatt ggatgcctct
     2161 tgtttgaatt ggctgattcg tgagacaggc atcaggcttt cctgaaccag tttcggataa
     2221 aaaaacggct cgccttcata tcgagatagt gaatctgcga gtcgcgatag tcgaaactgc
     2281 aatagatcaa acccgccaga ttggcaatcg gtcccggaaa acatgctacc gcccaagctt
     2341 tgaac
//
LOCUS       DATMTI010001014         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_802727, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001014 DATMTI010000000
VERSION     DATMTI010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802727"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VN039_16025"
     CDS             <1..726
                     /locus_tag="VN039_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWV47504.1"
                     /translation="RGSLTDHQAGEVLALIRPLGHLDRLRDVQMVIEAVPEDLAVKQE
                     LFAQLNRICVPQAVLASNTSSISITKLGAASGRPDRIVGLHFMNPAPVMRLVEVVRGL
                     ETSERTTQLALDLAKCLGKTPVVSKDVPGFIVNRVLIPMINEAIFALEEGVASAEDID
                     LAMTAGANHPVGPLALADRIGLDTVLAICDVLHQDLGDPKFRACPLLRRYVEAGWLGR
                     KCGRGFHVYEGKNERQEAISGTS"
     gene            767..2362
                     /locus_tag="VN039_16030"
     CDS             767..2362
                     /locus_tag="VN039_16030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWV47505.1"
                     /translation="MQERKPRFISLSGFDTNRVYTHNDLKDWSPDEELGAPGQFPYTR
                     GVYPTMHRGRLWTMRQFAGFGSAEDTNRRFKYLLQHGQTGLSVAFDMPTLMGIDADDP
                     RAHGEIGHCGVAISSLDDMDRLFEGIPLDQVTTSMTINGPAAVIFAMYLAVAEKRGIH
                     LEQLQGTLQNDILKEYIAQKEWLFPPEPSIRLITDTIAYCCEHVPKWHPVSISGYHIR
                     EAGSTAVQELAFTLYDGLTYVEAAIKAGLSVDRFAPQLSFFFNAHNDFFEEIAKFRAA
                     RRLWAREMMRRYRPGDPRSVQLRCHAQTAGCSLTAQQPMNNVVRTTIQALAAVLGGTQ
                     SLHTNSMDETLALPTEGAVKLALRTQQIIAQESEVTNSVDPLGGSYYVETLTNRLEEA
                     AQDYFHRLDDMGGMVKAIERGFPQREILDASQRYQRELERKERIVVGINEYVEPEERP
                     IPTLKIGQEVEQEQVARLSDLRKSRDSFRMAGAVEALQEAASCGENVMPSLIDAVKAM
                     ATLGEICVALKEVLGAYREPVVL"
BASE COUNT          514 a          663 c          713 g          519 t
ORIGIN      
        1 aggggaagcc ttaccgatca tcaagccgga gaggtcttgg cgctcatccg accgctgggg
       61 catcttgacc gcttgcgtga tgtgcagatg gtcatcgaag cggttccgga agatctcgcc
      121 gtgaagcagg aactctttgc ccaattgaat cgaatctgcg tacctcaggc ggtgctcgcg
      181 agcaatacct catccatttc aatcacaaag ttaggagccg cctccggtcg gcctgaccgc
      241 atcgtcggtc tgcatttcat gaatcctgcg cccgtgatgc gtctcgtgga agtcgttcgc
      301 ggactggaaa catccgaacg aacgacgcag cttgcactcg acttggcaaa gtgtttgggg
      361 aaaacgccgg tcgtgtctaa agatgtcccg ggattcatcg tgaaccgtgt gctgattccc
      421 atgatcaatg aagcgatttt tgccttggag gaaggcgttg cgtctgcgga agacatcgat
      481 ctggcgatga ccgccggcgc caatcatccc gtagggccac tggcccttgc cgaccgcatc
      541 ggtttggata ctgtgctggc catctgcgat gtcttgcatc aagacttggg tgatcccaaa
      601 tttcgcgcct gccctttact ccgtcggtat gtcgaagcag gatggttggg acggaagtgc
      661 ggacgcgggt ttcatgtcta cgaagggaag aatgaacggc aggaagcgat aagcggtaca
      721 tcctgatctt ggtccttggg tcctcagcac gttctggagc agatcgatgc aagaacgtaa
      781 accaagattc atttccctct caggattcga caccaatcgg gtgtataccc acaacgattt
      841 gaaggactgg tcgccggacg aggagcttgg cgcgccgggt caattcccgt acacgcgagg
      901 ggtgtatccg actatgcatc gtggccggct ctggaccatg cgacagttcg ccggcttcgg
      961 atcggcggag gacaccaacc gccggtttaa atatctcctc caacatgggc aaaccggcct
     1021 gagtgtcgcg tttgacatgc ccacgttgat ggggatcgat gcggatgatc cacgcgctca
     1081 cggcgagatc ggtcattgtg gtgtcgcaat ctcgtcgctc gacgatatgg atcgactgtt
     1141 cgagggcatt ccgcttgacc aggtcaccac gtcgatgacc atcaatggtc cggccgcagt
     1201 gatctttgcg atgtatctcg cggtcgcgga gaagcgcggc atccatctcg aacagctgca
     1261 aggcacgttg cagaatgaca ttctgaagga atacatcgca cagaaagagt ggctctttcc
     1321 tccggaaccc tcgatccgtt tgatcaccga caccatcgcg tactgttgtg agcatgtccc
     1381 caaatggcat ccggtcagta tcagcgggta tcacatccga gaagccggct cgactgcagt
     1441 gcaggaactg gcctttaccc tatacgacgg cctgacctat gtggaagctg cgatcaaagc
     1501 cggtctttcc gtggaccgat tcgctccgca gctctcgttc tttttcaacg cccacaatga
     1561 ctttttcgaa gagatcgcca agttccgtgc cgcccgtcgg ctatgggctc gcgaaatgat
     1621 gcgccggtat aggcccggcg atcctcgctc ggtacagctc cggtgccatg cgcagacggc
     1681 tggctgttca ctcacggcac aacagcccat gaacaacgtg gttcgcacaa cgattcaggc
     1741 gctggcggcg gtcttgggtg gcacgcagtc gctccatacc aattcgatgg acgaaacgct
     1801 tgcgttgccg accgaaggcg cggtgaaact ggcgcttcgc acgcagcaga tcattgcgca
     1861 ggagagcgaa gtcaccaata gcgtcgatcc tctcggcggt tcttattatg tcgaaaccct
     1921 cacgaatcgc ctggaggaag cggcacagga ttactttcac cgattggatg acatgggcgg
     1981 catggtcaag gcgatcgagc ggggattccc tcagcgtgaa atcctcgatg cctcccaacg
     2041 gtaccaacgc gagttggaac ggaaagaacg gatcgtcgtc ggtatcaacg agtatgtgga
     2101 gccggaggaa cgtccgattc cgactctcaa gataggacag gaagtcgaac aggaacaagt
     2161 ggcgcgtctt tcggatctac ggaagtcgcg cgattcattc aggatggccg gcgccgtcga
     2221 agcgttgcaa gaagcggctt cttgcggtga aaacgtcatg ccctctctca ttgacgcggt
     2281 caaggccatg gcgacgttag gtgaaatctg cgtggccttg aaagaagtgt tgggcgcgta
     2341 tcgggagccg gtggtgttgt aatggcggcg atgatcagga tgcctcctgt gctcgcgcga
     2401 cgcgcacac
//
LOCUS       DATMTI010001015         4961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_165649, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001015 DATMTI010000000
VERSION     DATMTI010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165649"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VN039_16035"
     CDS             <1..992
                     /locus_tag="VN039_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HWV47506.1"
                     /translation="ALRTWARGLGVETFVGTSGRIFPIDLKAAPLLRAWLRRLRQAGI
                     QFHIRHRWCGWDQEGSLLFTTPYGPVHAKADAVVLALGGGSWPHFGSDGAWVSIMSER
                     KIPIAPLKPANCGFDVQWSEHFRTKFSGYPVKTVEVAVKTVDGIVTRHRGEFVITANG
                     VEGGVIYMVSAAARNVIAAEGTVTLWVDLVPDRTLQRLTKDFAEPRGKRTVATHLKRC
                     AGIAGVKAGLLRDVLSKDVPADPVRLAAVIKSLPLTLVVPRPLEEAISTAGGVSFDAL
                     DRRLMLRSFPGVFCAGEMLDWEAPTGGYLLTGCLATGCLTGRAAAEWADEHCG"
     gene            complement(1033..1746)
                     /locus_tag="VN039_16040"
     CDS             complement(1033..1746)
                     /locus_tag="VN039_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47507.1"
                     /translation="MTRPTGIMSLVAQRVVLAMLAVALLLEVGCVSRRTYEKVKAEML
                     EQTQALEAVREDVRELDREIAGLQASKRREDAAVSVLRADIQREEEQLPIMKQRAEDT
                     LASLKTQVATLMNQSWHLARKIVDIRQESVSLQTKVAQYKEEMERVRAPVMVASDRNK
                     AAITQAADSEVTPSSVASTQDAESTQVAEAALPPPGLAPVKPSVSAPPMNADPPQAND
                     SWLGIITSWFAKIWNWLFG"
     gene            1877..2509
                     /locus_tag="VN039_16045"
     CDS             1877..2509
                     /locus_tag="VN039_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47508.1"
                     /translation="MDNPPEFAYYEAIMSFTAATSIRLPPACETALLERFLKAETMAL
                     WTVRSARLQDVPPNVYAFLRKHEEDEQQHLAQFEAMIGRQPRERERLPSVPRQWPALA
                     VQLYGYESLGLEFATLLVTLRPDLVSIREDELVHVEFFEQEIRRLLKDGTSAAEQARV
                     SARAWWRKLPRTVDRYLEAAVLDPFRPVLAGRILSSIERRFTTMGLLKDG"
     gene            complement(2516..3487)
                     /locus_tag="VN039_16050"
     CDS             complement(2516..3487)
                     /locus_tag="VN039_16050"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HWV47509.1"
                     /translation="MRDYLEFEKPIREIEEKIEKLSATATNGKSSAQHDIRKLRTKLA
                     QTEHELYKNLTPWQRTQLARHPQRPSTLDYITELTRDFLELHGDRVFGDDRAIVGGFA
                     RFNDRPVMIIGHQKGKTLKDRMQRNFGMPNPEGYRKALRLMKLAEKFNRPIITFIDTP
                     GAYPGIGAEERGQAEAIARNLCVMSRMTVPIISVVIGEGGSGGALALAVADRVLMLEH
                     SVYSVISPEGCAAILWDSPEKVPDAAAALKMTAHDLLDLGVIDTIVSEPLGGAHREPR
                     AVCDLIGKALTNQLFDLLDLSVEQLIAQRDQKYRKMGAVTGLLPVQA"
     gene            complement(3573..>4961)
                     /locus_tag="VN039_16055"
     CDS             complement(3573..>4961)
                     /locus_tag="VN039_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWV47510.1"
                     /translation="YQEQVMAIANKMAGFSLGQADILRRAMGKKKPEEMEKLRAKFLD
                     GAKQKKIPEKKAEKLYELIQKFAGYGFNKSHAAAYAVVCYQTAYLKAHYPTEFMAALM
                     TTDMGNQDKLVGYFTECRDLGIKVLGPDVNASQKHFAVAGGAIRFGLAAIKNVGEGAV
                     ESVLEVRAQAGPFGSFFDFCRRVDLHKVNKRMLEGLIKAGAFDSSGAKRAQLMAVLDQ
                     AVEEGAAAQRERELGQISIFGEEVNGHTVSPALPTPLLPSLAEWDQAQRLKYERELTG
                     FYISAHPLARYEATLSALSTTTTVRLKECSDGSEVKICGIIATVKSMLTKKGDRMAYL
                     TVEDLQGTTEVIVFPDLFRTAGELIAPERIVRITGTIDRGDKGTKIRGSKIEPLAEVQ
                     AQSIKRIRIRLTDRPEVREQLPRLLDIFRRHPGSTSISMSFRTDGTLEAETAPLPHLT
                     VCASDHFVSDVE"
BASE COUNT          996 a         1381 c         1427 g         1157 t
ORIGIN      
        1 cggctcttcg gacttgggct cgcgggctgg gcgtggaaac attcgtcggt acgtccggac
       61 ggatctttcc tattgatttg aaagctgcgc cgctcttgcg tgcatggttg cgtcggttga
      121 gacaagctgg catacagttt catatcagac atcggtggtg cgggtgggat caagaaggat
      181 cgctgctttt taccactcca tatggaccgg tgcatgctaa agccgacgct gtggtgctgg
      241 cgttaggcgg aggtagctgg cctcatttcg gttccgatgg agcatgggtt tcgatcatga
      301 gtgagcgaaa gatacctatc gctccgctga agccggccaa ctgtggattt gatgtacagt
      361 ggagcgagca ctttcggacg aagttttccg gatatccagt gaagacagtt gaagtcgccg
      421 tgaagaccgt ggacggcatc gtgacccgtc acagggggga gttcgtcatt acggcgaacg
      481 gcgtggaagg cggcgtcatc tatatggttt cagccgccgc ccgtaatgtg atcgcagcgg
      541 agggaacggt aacgttatgg gtagatctcg ttccggatcg aacgcttcag cggctcacaa
      601 aggattttgc agagccgcgt ggtaaacgta cggttgcgac gcatctcaaa cggtgtgcgg
      661 gtattgccgg agtcaaggcg ggtctcctgc gcgacgttct gtcgaaagat gtgccggcag
      721 atcctgttcg tctcgctgct gtcatcaaat ctctacccct cacgctcgta gtccctcgcc
      781 cattggaaga agccatcagt acggccggtg gcgtctcttt cgacgcactg gacaggagat
      841 tgatgcttcg ttcatttccc ggggtgtttt gcgccggaga aatgttggac tgggaggcac
      901 cgaccggagg atatctgctg acgggttgtt tggcgaccgg atgtctcaca ggccgtgcag
      961 cggccgaatg ggccgacgaa cattgtggat gagaagtatg actctcgttc gatcaggtgc
     1021 gaagagcccg tcctatccga ataaccaatt ccagattttg gcgaaccagc tggtgatgat
     1081 tccgagccac gagtcgtttg cctgaggcgg atcggcattc atgggaggtg ccgagactga
     1141 tggtttgaca ggcgcgaggc cgggaggagg gagagcagct tctgcgacct gtgtcgattc
     1201 ggcgtcctga gtcgacgcta ctgaagatgg agtcacttca gagtcggcgg cctgggtaat
     1261 ggcggctttg ttcctatctg aagccaccat cacaggcgct ctgacccgtt ccatttcctc
     1321 cttgtactga gccaccttgg tttgcaatga aacgctctcc tgtcgaatat ccactatctt
     1381 gcgcgccaga tgccaacttt gattcattaa ggtcgccacc tgtgttttga gcgacgcgag
     1441 ggtatcctcc gctcgctgtt tcatgatcgg caactgttct tcttcccgct gaatatctgc
     1501 gcgcaatacg gacacagcgg cgtcttcacg tcgcttcgaa gcctgcaatc ccgcaatctc
     1561 ccggtccagc tctctgacgt cctctcgtac cgcttctaag gcttgggtct gttccaacat
     1621 ctccgccttg actttttcgt acgttcgtcg gctgacacag ccgacttcca agagcaaggc
     1681 cacggcgagc attgcgagaa caacccgttg agcgaccaga gacatgatac cggtaggacg
     1741 tgtcataggt gttgcctcat gggttagcca acccgaaact cttgtgccgg gtcagcagtc
     1801 ggtccggtga gctgcgtaag atgtacacag atgatgagtg tcagtcaacc ctcctcattc
     1861 ctcctttcag gagagattgg ataacccgcc ggagtttgcg tactatgagg ccatcatgtc
     1921 gttcaccgcg gctacatcca ttcggctccc accggcgtgc gaaaccgctc tattggaacg
     1981 gtttcttaaa gccgaaacca tggcgctttg gaccgtacgg tcggcgcgtt tacaagacgt
     2041 tcctccgaac gtctatgcct ttctcagaaa acacgaggaa gacgagcagc agcatctggc
     2101 acaatttgaa gccatgatcg gccgtcagcc tcgcgaacgg gaacggcttc catccgtgcc
     2161 acgacaatgg cctgcgctgg ccgttcagct ctatgggtat gagtcactgg gacttgaatt
     2221 cgcgacgtta ctcgtcacat tacggccgga cttggtctcg atccgcgagg acgaacttgt
     2281 ccatgtggaa ttctttgagc aagagattcg ccgtctgctc aaagacggga cttccgctgc
     2341 cgagcaagcc agggtctccg cccgtgcgtg gtggcggaaa ttaccgcgaa cggtcgatcg
     2401 ctatctcgaa gcggcggtgc tcgatccgtt ccgccctgtg ctggcaggca ggattctttc
     2461 ttcgatcgaa cggcgattca ccacaatggg attgttgaag gacggatgaa gtcaatcagg
     2521 cttggacggg gagaaggccg gtgacggccc ccatctttcg gtatttctga tcccgctgag
     2581 caatgagttg ctcgacggac aaatcaagca gatcgaacag ttggttggtg agtgcctttc
     2641 cgatcaggtc gcagacggct cgcggctcgc gatgagcgcc gcccagcggt tcggaaacaa
     2701 ttgtgtcgat caccccgaga tcgagaagat cgtgtgcggt cattttcaat gcagcggcgg
     2761 catcgggaac tttctccgga ctgtcccaaa gaatggcggc acagccttca ggcgaaatga
     2821 ccgaatagac cgagtgttcc agcatcagga cgcggtcggc gaccgcgagg gccaaggcgc
     2881 ctccactccc tccttcaccg atgaccacgg aaataatcgg taccgtcatt cgggacatga
     2941 cacacagatt gcgggcgatg gcttccgctt ggcctcgctc ctcggcaccg atgccgggat
     3001 aggcaccagg cgtatcaata aaggtgatga tcgggcggtt gaacttttcc gccagcttca
     3061 tgaggcggag cgccttgcga tacccttccg ggttgggcat gccgaagttt cgttgcatgc
     3121 gatctttgag ggttttgcct ttttgatgac cgataatcat cacggggcgg tcgttgaacc
     3181 gggcgaatcc tcccacgatg gcccgatcat ctccaaacac acgatcgcca tgcagctcaa
     3241 gaaagtctct cgtcagctca gtgatatagt ccaaggtgct tggacgttgc ggatgtcggg
     3301 ccagctgtgt tcgttgccag ggggtcaggt ttttgtagag ttcgtgctcg gtttgagcaa
     3361 gcttcgtacg aagcttgcga atgtcatgtt gggcggacga tttcccattg gtggccgtcg
     3421 cagagagttt ctcgatcttc tcttcgatct ctcggatcgg cttttcaaat tcgagataat
     3481 cgcgcatagc ctgttcaatc ccgtacaacg atcagccaag gcacaacctg aggctcctaa
     3541 gatagcaaag agagtgcccc tttgcctagc acttactcaa catccgacac aaaatggtcg
     3601 ctggcacaga cggtaagatg agggagggga gccgtctcgg cttccagggt accgtcggtt
     3661 cgaaacgaca ttgagatcga ggtactgccg ggatgccgcc gaaagatgtc cagcaatcga
     3721 ggtaactgct cacgcacctc cgggcgatca gtgaggcgga tacggatacg tttgatggac
     3781 tgcgcctgga cctcggccag aggttcgatc ttgctgcccc gaattttcgt gcctttgtct
     3841 cctcgatcga tggtaccggt gatacggacg atccgttccg gtgcgatcag ctcgcccgca
     3901 gtcctgaaca aatctggaaa cacgatcacc tcagtggtcc cttgcaggtc ttccactgtg
     3961 agataggcca tccgatcgcc cttcttcgtc aacatcgatt tgactgtcgc gatgatgccg
     4021 cagatcttca cttcgctgcc gtcggagcat tccttcaatc tcacggtcgt tgtggtcgac
     4081 aaggcactca gcgtcgcttc gtagcgggcg aggggatggg cggaaatata gaacccggtc
     4141 agttcccgtt cgtacttcaa tcgttgtgct tgatcccatt ccgcaagcga cgggagcagc
     4201 ggagtcggta aggccggcga gacggtatgc ccgttcacct cctctccgaa gatgctgatt
     4261 tgtccaagct cacgctcgcg ttgagccgcc gcgccctctt ccacggcttg atcgagcacc
     4321 gccatcagtt gtgcgcgctt ggcgccgctc gaatcgaacg cgccggcctt aatcagacct
     4381 tccaacatcc gcttgtttac tttgtgaaga tcgacccgtc ggcagaagtc aaagaacgat
     4441 ccgaaggggc cggcctgagc ccgcacttcc agaaccgact ccacggcacc ctcgcccacg
     4501 ttcttaatgg ctgccaagcc gaacctgatc gccccgccgg ccacggcgaa gtgcttttgg
     4561 ctggcgttca catcgggccc caggactttg atgcccagat cccgacattc cgtgaaatag
     4621 ccgacgagct tgtcttggtt gcccatgtcg gtcgtcatga gggcggccat aaactcggtc
     4681 gggtagtggg ctttcaagta ggccgtctgg taacagacga cggcataggc ggcagcgtgc
     4741 gatttattaa aaccgtagcc ggcgaatttc tggatcaact catagagttt ctcggctttc
     4801 ttctcaggaa tcttcttctg cttcgcgcca tcgaggaact tggcacgcaa cttttccatc
     4861 tcttccggct tctttttgcc catcgcacga cgaagaatat cggcttggcc caacgagaac
     4921 ccggccatct tgttggcgat cgccatcacc tgttcctggt a
//
LOCUS       DATMTI010001016         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_674543, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001016 DATMTI010000000
VERSION     DATMTI010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674543"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1534)
                     /locus_tag="VN039_16060"
     CDS             complement(<1..>1534)
                     /locus_tag="VN039_16060"
                     /inference="COORDINATES: protein motif:HMM:NF016448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWV47511.1"
                     /translation="NGTERVVVSQLIRSAGVFFTADSTVGRNYYGAKLIPGRGAWLEF
                     ETATNGAMYVKIDRRRKLPVTTLLRALGHSKTSEIKSLFEDIDTGDTKYIEATLEKDP
                     TRGSNEALIEVYRRLRPGDLATVDNARQMIERMFFDFKRFDYSRVGRYKMNQRLGLEV
                     ANTTENRVFRMDDLIAIIREIIRLNNSQESADDIDALSNRRVKLVGELIARQFRVGML
                     RMQRNAMDRMSMSDIETVTPSQLINARPVVAAVREFFTSSQLSQLMDETNPLSELSHK
                     RRLSSLGPGGLSRERAGFDVRDAHPTHYGRLCAVETPEGANIGLVLNLASYARVNEYG
                     FIETPYLKVKNSKVTDEVVYLDASQEATEVIADAGAELSKDGTFANERVSARNGLLPE
                     QVDASEVTYMDAAHKQILGSSAALIPFIEKNRVDRSLTGSAMQKQAVPLLDPESPTVG
                     TGIEAEIAKNTSQLICAEADGEVVRADADEIQVKYKDGVKTYNPIHFTKSNDDRSINQ
                     KVR"
BASE COUNT          350 a          402 c          409 g          373 t
ORIGIN      
        1 cgcgtacttt ctgattgata gagcggtcat cattactctt ggtaaagtga atcgggttat
       61 atgtctttac gccgtcttta tatttgacct ggatctcatc agcatccgca cggacgactt
      121 caccgtcagc ttctgcgcag ataagctggc tggtattctt ggcgatctcc gcttcgatac
      181 cggtaccaac agttggagac tcaggatcga gcaacggcac agcctgtttc tgcatggcag
      241 aaccagtcag ggatcggtcg acacggttct tctcgatgaa tggaatcaga gcggctgacg
      301 aaccaaggat ctgcttgtgg gcggcatcca tgtacgtaac ttcgctggca tcaacctgct
      361 caggcagtag accgttacgg gcactgacgc gctcgttggc aaatgtaccg tctttgctca
      421 gttcggcacc ggcatcggcg atgacttcgg tcgcttcttg cgacgcgtcc agataaacga
      481 cttcgtcggt caccttactg ttctttactt ttagatatgg tgtctcgata aagccgtact
      541 cgtttacacg ggcatagctg gcaaggttca acaccagtcc gatgttcgca ccttccggtg
      601 tttccactgc acagaggcga ccgtagtgag tcgggtgcgc atcacgaacg tcaaagccgg
      661 cacgctcacg cgagagaccg cctgggccga ggcttgaaag gcgacgcttg tgtgatagtt
      721 cggacagcgg gttggtttcg tccatcagct gcgaaagctg tgaacttgtg aagaattcac
      781 ggacggcagc cacgacggga cgagcattga tgagctggct cggtgtaacc gtctcgatat
      841 cactcatgct catacgatcc atcgcgttac gctgcatacg gagcataccg acgcggaact
      901 gacgtgcaat caactcaccg accagtttga cccgacgatt agaaagtgcg tcgatatcat
      961 cggcagactc ctgagagttg ttcagacgga tgatctcacg gatgatggca atcagatcgt
     1021 ccatacggaa gacacggttc tctgtagtgt ttgcgacctc aagaccaagg cgctgattca
     1081 tcttgtaacg accgacacgg ctgtagtcga aacgtttgaa atcgaagaac atccgctcga
     1141 tcatttgacg agcgttatcg acagtggcaa gatcgcccgg gcgcagacgg cgatatactt
     1201 cgatcaatgc ttcgtttgag ccacgggtag gatctttttc gagtgtggct tcaatgtatt
     1261 ttgtatcacc ggtatcaatg tcttcaaaca aactcttgat ttccgaggtt ttactgtgac
     1321 caagcgcacg cagcagtgtc gtaaccggca gcttgcgacg tcggtcgatc tttacataca
     1381 tcgcaccatt ggtagcggtc tcgaactcaa gccaggcacc gcggccagga attagcttgg
     1441 caccataata attacggccg accgtgctgt ctgccgtgaa gaagacaccg gcactacgga
     1501 taagctgtga tacgacgaca cgctctgtgc cgtt
//
LOCUS       DATMTI010001017         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_845568, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001017 DATMTI010000000
VERSION     DATMTI010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845568"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="VN039_16065"
     CDS             <1..1716
                     /locus_tag="VN039_16065"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HWV47512.1"
                     /translation="LIMLGTPNYGSYAIAQAMVAKDKLVKWLAAADLRHDLEEVLDVL
                     NGFVGSYQLLPSPAKLADSERGLYDAATWGRYPIVGSHLNRARKFHMDLDVPATTDPE
                     RMTYIAGCNQETLNGVTIDAPGMFEFDMTLKGDGRVTHALGLLPSVPTYYVDEIHGDL
                     QKNEQVLEAIDEILRTGKTGVLASEPVAAREVRSVPSARVRAVQDRQDAEQIRRIAEE
                     TEVNRSTPAERRLAEDLLRQAIMTQAPPASRAVTDSAQAKAYGERMKQRPISMPISLN
                     LEAVHGDIRDIKTPAVVVGHYRGLPPVRAVGALDQALEFWISKAVKHGMIGGGLGEVF
                     LIPNTQKSIAANTLILAGMGEYGRFNREDLDLLFANVTYGITALGWREFATVVVGSGE
                     GNLSLEQAIEGMLVGVGSALRRLKDQGHLETLRIVEYDKDRFKAILKVLEKIKNNGDG
                     SLTVRLTTRELPKRTHRKNPSKPEHLDQMKGSRITIERDRDVFRFSAIREQAVIPVRE
                     ITIQSSYAEGAAELLKELRSVKDQQKYGKLLHSYLMPEDFQDMVDVTPLTLMVDRSTA
                     GSRGK"
     gene            1842..>2400
                     /locus_tag="VN039_16070"
     CDS             1842..>2400
                     /locus_tag="VN039_16070"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWV47513.1"
                     /translation="MLVIADPAPEAELQLPGAREEGRAVVQIMQNMKEEQELDIAIEQ
                     RIGANECDPVELLALILSEEFDLIHYSGHGDFDEKNPARSGWVFGKENILSARDIFRA
                     RRVPRLIFANACFSGVVRSGKPFTLQESNRNLAGLAQAFFERGVQNYIGTGWPVDDAA
                     ALEFAKIFYRDALSGNELGSSLSKAR"
BASE COUNT          537 a          649 c          740 g          474 t
ORIGIN      
        1 ctcattatgc tcggcacacc gaactacgga tcctatgcca ttgcgcaagc gatggtggcc
       61 aaggacaaac tcgtgaagtg gttggcggcg gcagacctgc gacacgattt ggaggaagtg
      121 ctcgatgttc tgaatggatt cgtagggagt taccaattgc tcccctctcc cgccaagctt
      181 gctgattcgg aacgaggtct ctatgacgcc gctacatggg gccgttatcc tatcgtgggg
      241 tcccatctca atcgcgcgcg aaagttccac atggatctcg atgtgccggc cacgaccgat
      301 ccggagcgga tgacctacat cgccggctgt aatcaggaaa ccctgaacgg ggtgacaatc
      361 gatgcccccg gcatgtttga atttgacatg accttgaaag gcgatggacg ggtgacgcat
      421 gcgctcgggc tactgcccag tgtgccgacg tactatgtcg atgagatcca tggtgatctc
      481 cagaagaatg agcaggtgct ggaagccatt gatgaaatct tgcgaaccgg caaaacgggc
      541 gtgctggcgt cggaaccggt cgccgcccgt gaggtccgct cggtgccgtc cgctcgtgtc
      601 cgcgccgttc aagatcgcca ggatgccgag cagatccgcc ggatcgcgga agagaccgag
      661 gtcaaccgaa gcactcccgc cgaacgccgt ctggccgaag acttactcag acaagccatc
      721 atgacgcagg cccctcctgc atcccgggcg gtgacggatt cggcgcaggc gaaggcatat
      781 ggagagcgga tgaagcaaag gccgatatca atgcccatct cgctgaacct agaggcggtc
      841 catggcgaca tacgcgatat caagacgccg gcggtggtcg tgggacacta tagaggtttg
      901 cctccggtgc gagccgttgg ggcgctcgat caggcgctgg agttctggat ttcgaaggcg
      961 gtcaagcacg gcatgatcgg aggcgggctt ggggaagtgt ttctcattcc caatacgcaa
     1021 aagtccattg ccgccaacac ccttattctc gccgggatgg gagaatatgg acgtttcaac
     1081 cgggaagacc tcgatctctt gtttgccaat gtcacctatg gcatcaccgc gctcggatgg
     1141 cgtgaattcg ccacggtggt cgtcggctct ggagaaggga atctctcatt ggagcaagcg
     1201 atcgagggca tgctggtcgg cgtgggatct gcgctcagac ggctcaagga tcaaggacat
     1261 ctcgaaaccc tgcgcattgt tgagtatgac aaagaccgct tcaaggccat tctgaaggtc
     1321 ttggagaaaa tcaagaacaa tggcgatggc tcgctgacag tccggctcac gacgagggaa
     1381 cttccgaagc gaacccatcg caagaacccg tcgaagccgg agcatctgga ccagatgaag
     1441 gggagtcgca ttacgattga acgggatcgt gacgtattcc ggttctctgc catcagagag
     1501 caggcggtga ttcccgttcg agagatcacg attcaatcct cctatgcgga aggagcggcc
     1561 gaactgttga aggaattgcg aagcgtgaag gatcagcaga agtacgggaa actcctccac
     1621 agctacctca tgccggagga ctttcaagac atggtcgatg tcacaccgct cacgctcatg
     1681 gtcgaccgtt cgacagcggg ttcccgtggg aaatagcggc gttcgagcag catggaaagg
     1741 ctattttcta cggaccggga cggcaattga cgcgacaatt ccaaaccacc ttgtccggtc
     1801 cgccgggact tactgcgccg acgactgccg agcgactacg cgtgctggtc atcgccgatc
     1861 cggctccgga ggccgagttg caattaccgg gcgcgcggga agaggggcgc gcggtcgtac
     1921 aaatcatgca gaacatgaag gaagaacagg aactggatat tgcgatcgag caacgcatcg
     1981 gcgcgaatga atgcgatccg gtcgaactgc tcgcgctgat cctgagcgag gagttcgacc
     2041 tgattcatta ctccgggcac ggtgacttcg acgagaaaaa tcccgccagg agtggatggg
     2101 ttttcggcaa ggagaacatc ctatcggccc gggacatctt ccgcgcgcgg cgagtgccgc
     2161 gcctgatctt tgccaatgcc tgcttctccg gcgtcgttcg atcggggaag ccttttacct
     2221 tacaggaatc gaatcgaaac cttgccgggc tggcccaggc gtttttcgag cgaggcgttc
     2281 agaattacat cggaacaggt tggccggtgg atgatgctgc ggccttggaa ttcgcaaaga
     2341 tcttctacag ggacgcgctg tccggcaatg agcttggaag ttctttatcg aaggcgagag
//
LOCUS       DATMTI010001018         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_759471, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001018 DATMTI010000000
VERSION     DATMTI010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759471"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VN039_16075"
     CDS             <1..533
                     /locus_tag="VN039_16075"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV47514.1"
                     /translation="SGKVAYVYPTLNTETRTVRVRLEFQNPGLKLKPGMYGNVTFQTD
                     AVRTLVVPKEAVLDTGLRQLVFVDRGQGRYEPASVELGRRSQDVVEVREGLKEGDRIV
                     TSANFLLDAESKLASASSMLDMMGRIGMGDWQMRGAHEGKMQGRRDMEGMEGIEMGKG
                     GVAAPPGREGEKGGMK"
     gene            530..>1913
                     /locus_tag="VN039_16080"
     CDS             530..>1913
                     /locus_tag="VN039_16080"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV47515.1"
                     /translation="MIARLIEGSARNPVLVIFCVLLLTGWGLWALFQVPLDAIPDLSD
                     VQVIVYTEWQGRSPTLIEDQITYPVVTSLLAGPKVKWVRGVSEYGVSYVYVIFEDRTD
                     LYWARSRVLEYLQKLTGKLPLGVTPTLGPDATGVGWVYQYALVDESGTYDLAQLRSLQ
                     DWYLRYQLESVPGVAEVSSIGGFVKQYQIEVDPNTLAVYRLPIQRVIEAVRNSNAEVS
                     GRVLEMAGTEYVIRGRGYLRSIDEIELIPVGTDGRGTPILVRDIAHVQLGPDQRRGIA
                     ELDGKGQTVGGIVIMRAGENALAVIERIKSRLEEIAPALPEGIRIVPTYDRSDLIHRA
                     IAVLREKLLEESVIVSLIAVLFLFHFRSALVAILILPVAVLLAFIPMAYLKITSNIMS
                     LGGIAIAIGAMVDAAIVMVENAHKRLEQNPPGKEARPMGRGPDLAGSEREGVTTPGAG
                     GCESDLAEIII"
BASE COUNT          411 a          472 c          628 g          402 t
ORIGIN      
        1 tcagcggcaa ggtggcttat gtgtacccga cgctgaatac cgaaactcgt acagtacggg
       61 tgcggttgga atttcagaat cctgggttga aattgaagcc gggcatgtac ggaaacgtga
      121 cttttcagac ggatgcggtc aggaccttag tggtgccaaa ggaagccgtg ttggacacgg
      181 ggcttcgtca actcgtattc gtggaccggg ggcaaggccg gtatgagcct gcttcggtcg
      241 agttggggcg ccggagtcaa gatgtagtag aggtgaggga aggactcaaa gagggggatc
      301 ggatcgtgac ctcggccaat ttcttgttgg atgcggagag caaattagca tcggcctcga
      361 gcatgctgga catgatgggc cggatcggca tgggcgactg gcaaatgcgc ggcgcacacg
      421 aaggcaaaat gcaaggcaga agagatatgg agggcatgga aggcatagaa atgggaaagg
      481 gaggtgtcgc ggcgccccca gggcgggagg gtgagaaagg cggcatgaaa tgatcgcgcg
      541 actcatcgaa gggagtgccc gcaatccggt cctcgtcatc ttctgcgtgt tgttgctgac
      601 aggctggggc ctatgggcct tgtttcaagt cccgctggat gccattccgg acctttcgga
      661 cgtgcaggtg atcgtctaca ccgagtggca ggggcgcagt cccacgctta ttgaagatca
      721 aatcacctac ccggtcgtga cctctctgct tgctggacct aaagtcaaat gggtccgagg
      781 tgtttcggaa tacggtgtct cctatgtcta tgtgattttc gaagaccgga cagatctcta
      841 ctgggcaaga agtcgtgtct tggaatatct ccagaagctc accggcaagt tgccgctagg
      901 cgtgacgccg accttgggtc ctgatgcaac cggagtcggg tgggtctatc agtacgcatt
      961 ggtagacgag tccgggacgt acgatctggc gcagcttcgg agccttcagg attggtatct
     1021 acggtatcaa ctggagagtg tgcctggtgt agcggaagtc tcatcgatcg gtggattcgt
     1081 caaacaatac caaatcgaag tggaccccaa cacattagcg gtttatcgcc tgccgattca
     1141 gagggtcatc gaagccgtcc gcaacagcaa tgcggaggtg agcggccgcg ttttggaaat
     1201 ggccggcaca gaatatgtga tccgagggcg aggctacctg cgctcgattg acgagatcga
     1261 gttgattccg gtcggcacgg atggacgagg cacgccgatt ctcgtgcggg acatcgcgca
     1321 tgtccaactc gggccggatc agcgtcgggg catcgccgaa ttggacggca aaggccagac
     1381 cgtcggcggg atcgtgatca tgcgggccgg agagaacgcg ctcgccgtga tcgagcggat
     1441 caaatcaagg ctggaggaaa tcgccccggc cttgcccgag ggcatacgca ttgttcccac
     1501 ctacgatcgg tccgatctca ttcatcgcgc gattgccgtc ctccgcgaga aactgcttga
     1561 agaaagtgtg atcgtcagcc tgatcgcggt cctgttctta ttccacttcc gaagcgctct
     1621 ggttgccatc ctcatcttac cggtcgcggt gttgctggcc tttatcccga tggcctactt
     1681 gaagattacg tccaatatca tgtcgttggg agggattgcc attgctattg gagccatggt
     1741 cgatgccgct atcgtgatgg tcgagaatgc ccataaacgg ctggagcaga atccccccgg
     1801 caaagaggcg cgtccaatgg gaagagggcc tgatctggca ggctcagagc gggagggtgt
     1861 tacgacgccg ggggcgggcg ggtgtgaaag tgacctggcg gaaatcatca tcg
//
LOCUS       DATMTI010001019         7638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_292475, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001019 DATMTI010000000
VERSION     DATMTI010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292475"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VN039_16085"
     CDS             complement(<1..1090)
                     /locus_tag="VN039_16085"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV47516.1"
                     /translation="MTKPRVVILGGGFGGMYAALEFERALARGAALDVTLVNQDNFFL
                     FTPMLHEVAASDLDITNIVSPIRRLLRRVTFFHGDIETIDLVHKRVGLSHGHETHCHS
                     LPYDHLVLALGSTTNFFNIPGLAGRALTMKSLDDAIVLRNHLIANLEEADYECGASLR
                     APLLNYVVAGGGFAGVETIAAMNDFLREALRYFPHLREDMFRIILVGAGKFILPELGE
                     KLGAYAQRKLREQKVEIHANCKVTAVTDRDVTLSDGTTVPTNTLVWTAGISPHSLLDT
                     LPCPKTKGRVLVNEYLEVPGWPSLWAFGVCALVPDLKTGGSHPPTAQHALREGRVAAQ
                     NILATVRGDRMKPFLYSTLGLLAPIGKRT"
     gene            complement(1087..1416)
                     /locus_tag="VN039_16090"
     CDS             complement(1087..1416)
                     /locus_tag="VN039_16090"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HWV47517.1"
                     /translation="MKPNNLIQKHDVHIATTATDSPNGSGDQQHRRIAAAAFALYERR
                     GRQDGHELDDWLAAEQQILLVEVAKTFEVAQRTDREARLRRENARLKRLVGELISELN
                     NNEEVWA"
     gene            complement(1893..3173)
                     /locus_tag="VN039_16095"
     CDS             complement(1893..3173)
                     /locus_tag="VN039_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWV47518.1"
                     /translation="MSDTHSILPPSPQLVEESLHAQRNRRLLIVALVLSLAGLAYGGY
                     WWTTARHWVQTDNAYVTGNLVPVTAQAGGIITQVLFEETQFVNRGDVLVRLDANEAAA
                     TLGQARGRLGDAIRRINSLFITQRQLADKLAARRARHDVIRHDLDRYRRAVPSGAVSK
                     QILENALDHLRALEADVRETQAEYDALDAQIGGTTVMEHPTVELAKHEFIHAHLEYAR
                     QQIRAPASGYVAKRKAQVGERVKHGTNLMTIVPLDHLWVEANLRETDLVDVRPGQPAE
                     VRVDLHGGKHTFHGTVEGLVPGTGSPFALLPPDNSTGNFIHIIERVPVRIALGAEDLR
                     EHPIRPGLSTVTKIRVSDVGQSIWSSLAKADTEEYETDVYDNELMDAESLAQEVMRTN
                     MVSRIASSALKPSDENTRRQTVVSYGLESADEKR"
     gene            complement(3178..4689)
                     /locus_tag="VN039_16100"
     CDS             complement(3178..4689)
                     /locus_tag="VN039_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWV47519.1"
                     /translation="MIEDQATISPYVSGLCLSVMFAVTGCGWWIPKGDPPATYMVSPE
                     LQETLEEVTNRLHKWPDDRWWQQFQNAELNQLMETALRDNPGLKVAAARLREAQGLAR
                     VEGARLLPFLDADASLTYERISQHGVFAALNPETAGQRILLGMINPLTFRYEFDFWGK
                     YRATLEAALGHAAAEEAELAEVRLRLTTGIARAYFRGHALHRQLELVHAIVDLRRSLR
                     ILAETRSKLGLDNDLAVKQAIADYEAAVTRQASVHDQLDIQCNLLARLIGKGPDDGRH
                     LFTASSSTIPEQVPVPEHLSIGLLVHRPDLAAALYRAYAAARLVKVARTQFYPTIDLT
                     AFVGFNALTFTKGADKLANFLFSGQSFSYGVAPGLRLPIFEGGRLRGELAVHRAEYDA
                     AVELYNDTLLDALREVADSLSAWQATREMLESHHRLVTSLSEDWRLAKVRVVSGLDDD
                     REVLRHHYPVLEQEYALRALESDQLVAVVDLIEALGGGYDNPDVEKKPEEPTS"
     gene            complement(4686..6284)
                     /locus_tag="VN039_16105"
     CDS             complement(4686..6284)
                     /locus_tag="VN039_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWV47520.1"
                     /translation="MTTCDHPETRCLGWHFILLNLVLGLAHIVVLFNAGSYVALEPHA
                     AGGLGGVLVSFGRWAQTNFMIALALGFPLARWLSDRYGEPRVFSAAFIAYAVASVFCA
                     TADGIEGFVSARVLLGLAGGVTLPLSQSLLIKEYPDHLKSLGLAIWGLFTLMPFTLGL
                     VTGGWIADHWGWRALFHLNILLAVLIAALAAVLFHGKRHDVRHEPFDLVGFVLLFVIF
                     GSLQTILNGGNDFDWFDDPLLRVMLVVVIVAVPVWIVWELGERRPAIDLRLFAHRNFS
                     VGLLCLGLGFLSIQGLLSLFVVQLQLLLGYSSELAGLVFLPMILLGMPMIALMHEIAK
                     RLDVRWLACLNSLGFAATFYWIGLFDDPHSYDQIFWPMVLEGVFLGSFFTPLTVLTLH
                     GLSGEQMLRAAETANILRIASGAMGITWQGIVVFRRLPFHQLQLSDHFGGRISASYDA
                     LNRFTTKLEALGFDPAMIQRKVQLTIKQAAGILALNDTFLLSSYLCLGIAALVWFAHS
                     SHVPVLKQAEAVRELQAEELMEQP"
     gene            complement(6597..>7638)
                     /locus_tag="VN039_16110"
     CDS             complement(6597..>7638)
                     /locus_tag="VN039_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV47521.1"
                     /translation="IDHVQAYEQIDQLRNRLAKENVYLNEELRLTKNIDRLVGESTVI
                     QHVLTLAREVAPTSTTVLLTGETGTGKELVAQTIHDLSQRHRKPMVRVNCASFPAGLV
                     ESELFGHERGAFTGADRTREGRFELAHEGTLFLDEIGEMPLETQAKLLRVLQDGMVDR
                     LGGKQPIRVDVRIIAATNRDLSAAVKIGTFRADLLYRLNVFPIRLPTLSERPEDIPLL
                     ARHFLELYAAKLLRSCTDIDPDSLERLVRYSWPGNVRELANVIERALILSRESILRID
                     NHLLGLQDRSFTSLPSSDLKDFERRHILQTLALTDWRIEGPDGAAARLGIPPSTLRSR
                     MKQLVIKRPTAD"
BASE COUNT         1615 a         2234 c         2232 g         1557 t
ORIGIN      
        1 cggtccgttt tccgatgggg gccagaagcc caagcgtcga gtagaggaac ggtttcatcc
       61 ggtcgcctcg aacggtcgcc aggatattct gcgccgcgac tctcccttca cgcagcgcgt
      121 gttgcgcggt tggggggtgg gatccaccag tcttgagatc cggcacgagc gcacagacac
      181 cgaaggccca gaggctcggc cacccgggca cttccaggta ctcgttgaca agcacccggc
      241 ccttcgtttt cggacagggc aaggtgtcca agagggaatg cggggagatt ccggccgtcc
      301 agaccagcgt attggtcggc accgtcgtcc catcgctgag cgtgacgtcg cggtccgtca
      361 cggcggtgac tttacagttc gcatggattt ccaccttctg ctctctgagt ttccgttgcg
      421 cgtaggcgcc gagcttctcc cccagttccg gcaagatgaa tttccctgca ccgaccagga
      481 tgatccgaaa catgtcctcc cgcagatggg gaaaataccg cagcgcctcc cgcagaaagt
      541 cattcatggc cgcgatggtc tcgacccccg cgaaaccgcc gccggccacg acgtaattca
      601 gcaagggtgc acggagcgac gccccgcact catagtccgc ctcttccaga ttcgcgatga
      661 ggtggttgcg cagcacaatc gcatcatcca acgatttcat ggtcagcgcc cggcctgcca
      721 agccggggat gttgaagaag ttcgtggttg agccaagggc cagcaccagg tggtcgtatg
      781 gcagcgagtg gcaatgggtc tcgtgcccat gcgacaggcc gacgcgcttg tgcacgagat
      841 caatggtttc gatatcgccg tgaaagaacg tcacgcgacg cagcagcctg cggatcgggc
      901 tgacgatatt cgtgatatcg aggtcgctcg cggcgacttc gtgcagcatc ggggtgaaga
      961 ggaaaaagtt gtcctggttg acgagggtca cgtctaaggc ggctccgcgc gccagcgctc
     1021 gttcgaattc caacgcggca tacattccac cgaacccacc acccagaatc accacgcgtg
     1081 gcttggtcat gcccacacct cctcattgtt gttgagttcc gagatcagtt cgcctaccag
     1141 cctcttgagt cgggcgttct cgcgccgcag gcgggcttca cgatccgttc gttgagcgac
     1201 ctcaaacgtc ttcgctacct cgactagaag gatttgttgc tcagcggcca accaatcgtc
     1261 caattcgtgg ccgtcttgcc gcccacgccg ttcatataac gcgaaggccg ctgctgcgat
     1321 acgccggtgc tgctgatcgc ctgatccgtt cgggctgtcg gtcgccgtgg ttgcaatatg
     1381 aacatcgtgt ttctggatca aattgttagg cttcatgggc acctccttgt atagttgacg
     1441 tccattcagt gatcgtttca accggagttg tgtgacatca cccgttccgt attccttcag
     1501 aaaaatacat gctactgtgc cggtcggttg aaatgttttc tcatgttttc cgactcatga
     1561 ccggatcgca atcgtgccgg gccagggccg gattccggtc ccagcagacc agatccaggg
     1621 cctatggacg gagacagggg gaacgtcggt gagccaagat ctgggcttcg tggcggaata
     1681 tgagactccg gtagggcgct tggtacacgt gaagtatgcg atccagtgtg agcgttcgaa
     1741 atgcctgtgg tgtcagctgg tctggtgggg tttgttggac gattgccgta caagtcagaa
     1801 gtttgaccat acaagacggc accatcggcc agaagagaca gggtcaaaat cgatccgaga
     1861 acgatatcct tcatcgccat tcctttcccg actcatcgct tctcgtccgc cgactcgaga
     1921 ccgtacgaaa caacagtttg tcggcgcgtg ttttcgtcgc tcggcttcaa cgcagatgaa
     1981 gcgatgcgag acaccatatt ggtcctcatg acttcttgtg ccagcgattc tgcgtccatc
     2041 aattcgttgt catagacatc ggtctcatat tcctcggtgt cggctttcgc cagagacgac
     2101 cagatggatt gaccgacatc gctgacgcgg atttttgtga ccgttgagag gccgggccta
     2161 ataggatgct cccgcaggtc ttcggctccc agcgcgatcc gtaccggcac ccgttcaatg
     2221 atgtggatga agttaccggt ggagttgtcc ggcggtaaca gcgcgaacgg actgcccgtg
     2281 ccgggtacca gaccttccac agtgccgtga aacgtgtgtt tgccgccgtg gaggtccacg
     2341 cgcacttcgg ccggttgacc cggtcggacg tccaccaggt cggtttctcg caagttggct
     2401 tccacccaga gatggtccag cggaacgatc gtcatgaggt tcgtgccgtg ctttacccgt
     2461 tcgccgacct gcgccttgcg tttcgccacg tatccggacg ccggcgctcg gatctgctgc
     2521 cgcgcatatt cgagatgggc gtggatgaac tcatgcttcg ccagctcgac cgtcggatgt
     2581 tccatcactg tcgtgccgcc gatctgagcg tcgagcgcgt cgtattcagc ctgtgtttcc
     2641 cgcacatctg cttccaatgc ccgcaagtga tccagcgcat tctctagaat ctgcttcgac
     2701 acggctccgc tgggcaccgc tcgacggtac cggtccaggt cgtggcggat cacgtcgtgc
     2761 ctcgccctgc gagccgccaa tttgtcggcc agttgccgtt gcgtaatgaa gagcgagttg
     2821 atgcgccgga ttgcgtcgcc gagtcgaccg cgcgcctgtc ccagggtcgc agcggcttcg
     2881 ttcgcatcga gacggaccag cacatcgcct cgattgacga actgagtttc ttcgaacagc
     2941 acctgcgtga tgatgccgcc ggcctgcgcc gtcaccggca cgaggttgcc ggtcacatag
     3001 gcattgtcgg tttgaaccca atgccgggcc gtcgtccacc aatatccgcc ataggcgagg
     3061 cctgccagac tcagcacgag cgccacgatc aacagccgcc ggttgcgttg tgcatgcagc
     3121 gactcttcaa ccagctgcgg cgacggtggg agaatggaat gggtgtcgct catacgttca
     3181 actcgtcggc tcctccggtt tcttttcgac atcgggattg tcgtatccgc cgcctaatgc
     3241 ctcaatgaga tccaccaccg ctacgagttg atcgctctcc agcgcccgca gagcatattc
     3301 ctgttccagc accggatagt gatgacggag aacttcccgg tcgtcgtcca gtccggacac
     3361 gacccgtact ttcgcgagac gccagtcttc gctgagcgac gtcaccaagc gatgatgcga
     3421 ttccagcatc tcacgcgtcg cctgccacgc gctcagactg tccgcgactt cccgcaaggc
     3481 gtcgagcagc gtgtcgttat acagttccac cgctgcgtcg tactccgccc ggtgcaccgc
     3541 cagttcgcct cgcaagcgtc cgccttcgaa gatcggcaag cgcaggccgg gcgcgacgcc
     3601 gtaggaaaag ctctgccccg agaacaggaa attcgccaac ttgtcggcac ctttggtgaa
     3661 cgtcaacgca ttgaagccga cgaaggcggt cagatcgatc gtcgggtaga actgcgtcct
     3721 cgcgaccttc accaaccgag cggcggcgta cgcgcgatac agggccgcgg ccaaatcagg
     3781 ccggtggacc agcaagccga tcgagaggtg ctcggggacg ggcacctgct cgggaatggt
     3841 cgaagaggaa gccgtgaaga ggtgtcgacc gtcatccggt cctttgccga tgaggcgggc
     3901 gagcaaatta cactgaatat ccaattgatc gtgaaccgag gcttggcgtg tgaccgccgc
     3961 ttcgtagtcg gcgatggctt gtttcaccgc caggtcatta tccaatccca gtttggaccg
     4021 ggtctccgcg agaatccgaa gactgcggcg tagatcgacg atggcgtgga ccaattccag
     4081 ctgacgatga agcgcatggc cgcggaagta ggcacgcgcg atgccggtgg tcagccggag
     4141 tcggacttca gccaattccg cttcttcggc tgccgcatgc ccgagcgccg cttcgagcgt
     4201 cgcgcgatat ttgccccaga agtcgaactc gtagcgaaac gtgagcgggt tgatcatgcc
     4261 gagcagaatg cgctgtcccg cggtctcggg attcaaggct gcgaacactc cgtgttgcga
     4321 gatgcgctcg taggtcaacg acgcgtcggc atccaggaag ggaagaaggc gtgcgccttc
     4381 cacacgtgcc agcccttgcg cttcccgtaa gcgagccgcg gccaccttga gcccagggtt
     4441 gtcgcgcagc gccgtctcca tcagttggtt gagctcggcg ttttgaaatt gttgccacca
     4501 ccggtcgtca ggccatttgt gcaaccggtt ggtcacttcc tcgagagttt cctgcaactc
     4561 gggcgacacc atataggtcg ccggcggatc gcccttcgga atccaccaac cacagccggt
     4621 gaccgcaaac atgaccgaca ggcacaagcc cgagacgtat ggggagatgg tcgcttggtc
     4681 ttcgatcatg gttgttccat cagctcttcc gcctgcaact cgcgcacggc ttccgcctgc
     4741 ttcaacaccg gcacgtggct ggaatgcgcg aaccagacca gcgccgcaat ccccagacag
     4801 aggtagctcg acagaaggaa cgtgtcgttc aaggcgagaa tgccggccgc ctgcttgatc
     4861 gtcagttgca ccttgcgttg gatcatggcg ggatcgaagc cgagtgcctc gagtttagtc
     4921 gtgaaccggt tgagtgcgtc atacgaagcc gagattctcc cgccgaaatg gtcgctgagt
     4981 tgcaattgat gaaaggggag acggcggaag acgacgatcc cttgccaggt aatccccatt
     5041 gcgccggacg cgatgcgaag aatattggcc gtctccgccg cacgaagcat ttgctcacct
     5101 gaaaggccgt gcaacgtcaa tacggtcaac ggcgtgaaaa acgagccgag gaagactccc
     5161 tcgagcacca tcggccaaaa gatctgatcg taggagtggg gatcgtcgaa gaggccgatc
     5221 caataaaacg tggcggcgaa tcccaagctg ttcaagcagg ccagccatcg gacatccagc
     5281 cgtttcgcga tttcatgcat cagggcgatc atcggcatac ccagcagaat catgggcagg
     5341 aacaccagac cggccagctc ggatgaatag cccagcaaca gctgtagttg aacgacgaag
     5401 agggagagca atccttgaat cgaaaggaag cccagaccga gacagagtaa gccgaccgag
     5461 aaattccgat gcgcgaataa ccgaagatct atcgccggac ggcgttctcc gagctcccag
     5521 acgatccaga ccgggacagc cacgatgacg acgaccaaca tgacgcgtaa aagcggatcg
     5581 tcgaaccaat caaagtcgtt tcctccgttc aaaatcgtct gaaggctacc gaagatcacg
     5641 aacaacagga caaatccgac gaggtcgaat ggctcatggc gaacatcatg acgtttccca
     5701 tggaacagga cggctgccag ggcggcgatc aacaccgcca ggaggatgtt gaggtggaac
     5761 agggcgcgcc agccccaatg atcggcaatc caaccgccgg tgaccagccc caaagtaaaa
     5821 ggcatcaacg tgaaaagccc ccagattgcc agacccaacg acttcaggtg atcgggatac
     5881 tccttgatca gcaaggattg gctcagcggc aaggtgacgc cgccggcgag accgagaagg
     5941 acgcgagccg acacgaagcc ttcaatgccg tcggccgttg cgcagaatac cgatgccact
     6001 gcgtaggcga tgaaggcggc actaaagacg cgtggttctc catagcggtc ggagagccag
     6061 cgggcgaggg gaaagcccag ggccagggcg atcatgaaat tcgtctgcgc ccacctcccg
     6121 aaactgacca acacgccgcc caagcctcct gccgcgtgcg gttccagcgc cacgtacgag
     6181 ccggcgttga acagcaccac gatgtgggct aggcccagca cgaggttgag gaggatgaag
     6241 tgccatccga ggcagcgggt ctctggatga tcacatgttg tcataagaag gctcgttcaa
     6301 cagaacgtgg tgtgctcggt tactcatctg ctgaggtctc ccgcacaaaa gtcgtctgtg
     6361 gtgcgtaagt cagcctcaac gtttgtagat gtgcgtgagc aagggctgtg cctcttcagc
     6421 cgcatagtct caacctagtg aaatttatgg tctgcgaggg attatctttt taaaccgaaa
     6481 aagaaaatta ctgagaaccc acgataggtc gcgattcatc gcggactcta ctcacgacgg
     6541 atcgaggttc tgatgctaag gggatggagg gtgtacgaga caggggcacg agtgcttcag
     6601 tcagcggtcg gtcgctttat cacaagttgc ttcatccggc tgcgcaaggt actgggcggg
     6661 atgccgagac gtgccgctgc tccgtcagga ccttcgatgc gccaatcggt caatgccaga
     6721 gtttggagaa tgtgtcgacg ttcgaaatcc ttgaggtcgg acgacggcaa gctggtgaac
     6781 gagcgatcct gcaaccccag gagatggtta tcgatccgca agatcgactc gcgtgagagg
     6841 atcaaggctc gctcgatcac atttgccaat tcacggacat tgcccggcca agagtagcga
     6901 accaatcgtt ctaaagaatc aggatcgata tccgtgcacg aacggaggag tttcgccgcg
     6961 tatagttcaa ggaaatgtcg ggccaacaat ggaatatctt ccggacgctc gctcagggtg
     7021 ggcagtcgga tcggaaacac gttcagacga tacagaagat ccgctcggaa cgtaccgatc
     7081 ttgaccgctg cagaaagatc gcggtttgtg gcggcgatga tacgaacatc cactcgaatc
     7141 ggctgtttcc cacccaaacg atcaaccatc ccatcctgga ggactcgcag caatttcgcc
     7201 tgtgtctcca gcggcatttc accgatttca tccaagaaca atgttccttc atgggccagt
     7261 tcgaatcgtc cttcacgggt gcggtccgct ccggtaaatg cgccgcgttc gtggccgaat
     7321 aattcacttt caaccagtcc ggccggaaag gaggcacaat tcacgcgaac catgggttta
     7381 cgatggcgct gactcaaatc atgaatggtt tgagcgacca attccttgcc ggttcctgtc
     7441 tcacccgtca ggaggacggt cgtcgacgtc ggggcaactt ctcgcgcgag agttaagaca
     7501 tgttgaatga cggtactttc gccgaccaat ctgtcgatgt tttttgtgag tcgcagttcc
     7561 tcgttcaagt acacattttc ttttgcaagt cggtttcgta attgatcgat ttgttcgtag
     7621 gcttggacgt gatcgatc
//
LOCUS       DATMTI010001020         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_548328, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001020 DATMTI010000000
VERSION     DATMTI010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548328"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="VN039_16115"
     CDS             <1..115
                     /locus_tag="VN039_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWV47522.1"
                     /translation="CRFWDMTTGESMDKDRFRKDMGGIEDAYQEVLKRVCG"
     gene            294..572
                     /locus_tag="VN039_16120"
     CDS             294..572
                     /locus_tag="VN039_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47523.1"
                     /translation="MISRDEAQSLLLVFGGKKEEMLRAYLNYGLIASVVIWAAIVGVM
                     AYRLNESPWRWAFVGLVFAGGLTVAVILWIKKYVDRLNEAEEKRESKS"
     gene            569..808
                     /gene="purS"
                     /locus_tag="VN039_16125"
     CDS             569..808
                     /gene="purS"
                     /locus_tag="VN039_16125"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWV47524.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAANVRVGKYMEL
                     DLDEKDRAKAEAEVKGMCEKLLANTIIEEYRFEIG"
     gene            901..1632
                     /gene="purQ"
                     /locus_tag="VN039_16130"
     CDS             901..1632
                     /gene="purQ"
                     /locus_tag="VN039_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWV47525.1"
                     /translation="MNIGVVVFPGSNCDHDCRYVFRDVLGQDVTMVWHKEASVTGLDA
                     VILPGGFSYGDYLRTGAIARFSPVMQAVKQFAAEGGLVLGICNGFQILLEAGLLPGVM
                     LRNRSLHFICRETYVKVENAATPFTNGCRPGQVLKIPIAHADGNYYTDPVTLAGLQAN
                     AQIILRYCTIEGKVTPEACPNGSLDNIAGIRNAAGNVLGMMPHPERCAEMVLGNEDGR
                     TIFQSMIASLERKQLAAVVSAPTTI"
     gene            1743..2081
                     /locus_tag="VN039_16135"
     CDS             1743..2081
                     /locus_tag="VN039_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47526.1"
                     /translation="MRRVGAVLAAVGLLTLGVGVNVWAAADDGSQVKITSPAEGAVVK
                     GGNGEIKYELTKGSKATHVHCFVDGEYQKGWKGMVKGMSPGTREIKLVAADKDHETLA
                     AESAIKVEVQ"
     gene            2251..>2642
                     /locus_tag="VN039_16140"
     CDS             2251..>2642
                     /locus_tag="VN039_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWV47527.1"
                     /translation="MPLITKDLIAQHNLTGEEYQKIVDILGREPNLTELGMFSVMWSE
                     HCSYKSSRMHLKKLPTTGPRVVQGPGENAGAVDIGDGLCVVFKMESHNHPSFIEPYQG
                     AATGVGGIMRDVFTMGARPIAMMNALRFG"
BASE COUNT          613 a          596 c          840 g          593 t
ORIGIN      
        1 ctgccgtttc tgggatatga cgaccggaga gtcgatggat aaggatcggt ttcggaagga
       61 tatggggggg atcgaggacg catatcagga ggtgttaaag cgggtgtgtg gatagtggag
      121 acctgagcct tgtccgctca agcacctcaa ctcgaccgca ctaagtttcg ccgtcgctcg
      181 gcacgcgaaa cttgttccgt tctaaagcgg agcttaaacg gaacatcgaa catcgcgagc
      241 cgtcccgcaa cgcggaccgc tcgggctcaa caagtgcggt ggcgcgagtt tccgtgatat
      301 cgcgggacga ggcccagagt ctcctcctgg tctttggggg gaagaaagaa gagatgctgc
      361 gggcgtattt gaattatggc ttgattgcat cggtggtgat ttgggcggcc atcgtgggcg
      421 tgatggccta tcgattgaac gaatcgcctt ggcgctgggc gttcgttggc ctggtatttg
      481 ccggcggact gactgtcgca gtgatcctct ggatcaagaa atacgtggat cggttgaacg
      541 aagccgaaga aaagcgggag agcaagtcgt gaaagccaaa attcatgtga cgctgaagca
      601 aggcatcttg gatccgcaag gcaaagctat cgaacatgcg ctggactcat tggggtttaa
      661 gaatgcggcg aatgtgcgtg ttgggaaata tatggagctg gatctcgatg aaaaagatag
      721 ggccaaggct gaggctgaag tcaaaggcat gtgcgaaaag ttattggcga ataccatcat
      781 tgaagagtat cggtttgaaa ttgggtagtg cggagggtgg agtgctgagc acagaggaaa
      841 aaggttctca aaattcagtc tgctgctcag cacttagcac tctggactaa aaacttgctt
      901 atgaacatcg gcgtcgttgt tttccccggt agcaactgcg atcacgattg ccggtacgtt
      961 tttcgtgacg tgctcggaca ggatgtgacg atggtgtggc acaaggaggc gtcggtaacc
     1021 ggtttggacg cggtgatcct gccgggagga ttttcgtacg gtgattatct ccggactgga
     1081 gcgattgcgc ggttttctcc ggtcatgcaa gccgtcaaac agtttgccgc cgagggagga
     1141 ttggtgcttg ggatctgcaa tggattccag attctgctgg aagcaggttt gttgcctggc
     1201 gtcatgttgc gaaataggtc attgcacttt atctgccgcg agacctacgt caaagtggag
     1261 aacgctgcga cgccgtttac gaacggctgc aggcccggac aggtgctcaa aattccgatc
     1321 gctcacgcgg acgggaatta ctacaccgat cctgtgacat tagccggtct tcaagccaat
     1381 gctcaaatca ttctccgtta ttgcacgata gaagggaagg tgacgccgga ggcttgtccg
     1441 aatgggtcac ttgataatat tgcgggtatc cgtaacgccg caggcaatgt cctgggcatg
     1501 atgccgcatc cggagcgctg cgcggaaatg gtactcggaa atgaagatgg gcgaactatc
     1561 tttcaatcga tgatcgcgtc tttggagcga aagcaattgg cagcagtagt gagcgctcca
     1621 acgacgattt aaccgagaat gctctccata cgattgaaga tggtagatgg tcgcggtaaa
     1681 gtgtggagtc agtcggtatg tatcagcctt aacttttttc atacatcatg aggagggttg
     1741 cgatgagacg tgtaggagca gtgttggcgg cagtcggtct gttgaccttg ggagttggtg
     1801 tcaatgtttg ggctgcggca gatgacggca gtcaggtgaa gatcaccagc ccggcagagg
     1861 gggccgtcgt aaagggtggc aatggtgaaa tcaagtatga gttgacaaag ggcagcaagg
     1921 cgacgcacgt ccattgtttc gtggatggag aatatcagaa gggatggaaa ggaatggtca
     1981 agggaatgtc acccgggact cgtgagatca agctcgttgc ggcagataag gatcatgaaa
     2041 ccttagccgc tgaatcagcc attaaggtgg aagtgcagta gaacaggatc ggggttcgtt
     2101 gatcacacag aacgactcgg tgaggcgccg actgtgcgcc tcgccggtca ccttgatcaa
     2161 tgcttgcaac ggtgtcacgc ataccctggt gttcggaacg ctccaaagcg ggtttgtctc
     2221 tgtgaccgct aggttgtaat gcaggccggc atgccgctca tcaccaaaga cctcatcgct
     2281 cagcacaatc tgacgggcga ggaataccaa aaaatcgtcg acattctcgg gcgtgagccc
     2341 aacctgacgg agctcggcat gttttccgtg atgtggtccg agcactgctc ctacaagagt
     2401 tctcgtatgc acctgaaaaa gctgccgacg accgggcctc gtgtcgtgca agggccggga
     2461 gaaaatgcgg gagcagtcga tatcggtgac gggctgtgtg tggtcttcaa gatggagtcg
     2521 cacaaccatc cgtcgtttat cgagccctat cagggcgcgg caactggcgt gggcggcatc
     2581 atgcgcgatg tcttcaccat gggcgcgcgg cccatcgcga tgatgaacgc gctgcgcttc
     2641 gg
//
LOCUS       DATMTI010001021         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_589829, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001021 DATMTI010000000
VERSION     DATMTI010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589829"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VN039_16145"
     CDS             <1..207
                     /locus_tag="VN039_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47528.1"
                     /translation="VEPAGGDLFEHATTLCVLGGDFQGRVFEDAEDLGDRLAHRFHRG
                     FRPLRRSGDDKAPQKSACKGRRPQ"
     gene            complement(274..474)
                     /locus_tag="VN039_16150"
     CDS             complement(274..474)
                     /locus_tag="VN039_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HWV47529.1"
                     /translation="MSVAKIIELSAESPTSFEDAVVQGIAKASKTVHGIKSAWVKEQH
                     VVVENNKVALYRVDLKVTFVLD"
     gene            998..>2435
                     /locus_tag="VN039_16155"
     CDS             998..>2435
                     /locus_tag="VN039_16155"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HWV47530.1"
                     /translation="MDKGASVVRTVRLLRVMIGLVPLLWVPVSLGQTTSITSSGLNTH
                     VSHTTGQQNYDITGGTRPDNGTNLFHSFGDFSVGVQDVARFVNDAHLPTTNILSRVTG
                     GNPSNIFGEINTTNFPGANLYLINPAGVLFGATATLNVSGSVHVSTADYLRLADGLRF
                     NAIPGPQDTLLSQAPVVAFGFLHAHPRPITVQGSRLSVGEGKVLSLVGGDIKVTGGTL
                     TASSGQINLVSLAGKGEARVAPDGIAIAGMTPRGQIVIKERFPRENHAPVAGLDTSGD
                     EGGAVVIRGGRLSLTGAEVIADARVANHTGRGIVIEATDSATMDNVSLRTSAFGVPNP
                     NTGGGAGPISVTAPSMTASNSSISTSSGNNGPAGDVAINVGSLTASNLQIETGIGSGN
                     RPAPASGNISIRATGDVTLQNSGISTGTFGLRDAGHITVRAATLALTEGSSISSGSQF
                     DGSSGGGRAGNIAIDVEHLTMRTSSSINT"
BASE COUNT          521 a          661 c          705 g          548 t
ORIGIN      
        1 gttgaacctg ccggtggcga tctattcgag catgcgacga cgctgtgcgt gcttggcggg
       61 gattttcagg gaagggtctt cgaggatgcg gaggacctgg gtgaccgtct cgcgcaccgc
      121 ttccatcggg gtttccgccc cttgcgaaga agcggtgacg acaaagcacc acagaaaagc
      181 gcatgcaaag gccgcaggcc acagtgatcc tcttgcagct cgccgacttc cttcggtcca
      241 ctggagcatg agatctaggt ccttcgtaac gcgtcaatcc aatacgaacg tcacctttag
      301 atctacacga tacaaagcca ccttgttgtt ttcaaccacg acatgttgct ccttcaccca
      361 agccgatttg atgccgtgga cggtcttgga tgctttggct attccttgga cgacggcgtc
      421 ttcaaaactc gtgggcgatt cggcgctgag ctcaatgatt ttagcgacgg acatggaacc
      481 cctccttttg ttcgtgcgca cccaacgttc tgatcccggc gattcatttt ctgatcgatt
      541 gtccagcaag gtcggcgcca ggacccgatc ggtcggcagc taggcacgta agcgatatct
      601 caggggaaat tgatcaggcg gaagtgagcc ttggtaggaa atggggagat ctgctacaag
      661 tcgaggccga gatctgtagc gaaagggggc gtactggaag tgatgaaact tgtccgatgc
      721 gcggaaaaga aatgtggacg atgtgcggaa atgcaatgtt gatggactgg ccaccctata
      781 ttgcctcaat aggtatccgc cacaggtggc aaggtacctt aatgcaaaca tcttatattt
      841 cctatattct gccttcgctt ctgcgtgagc cactgcaagt gctttgtgta acctgccgga
      901 gaaggtgctc tctggcagcg gacttgcttc actgagctgc cttttctctc gatcagcagt
      961 catttgagcc aacctcttct tgaaaaggag agattttatg gacaagggag caagtgtcgt
     1021 gcgaacagtg cggctgctcc gggtcatgat cgggttggtg ccgctactgt gggtgccggt
     1081 cagtctcggg caaaccacct cgattacgtc ctccggactg aacacccatg tttcacatac
     1141 gaccggccaa cagaactacg atattacggg gggcacgagg ccggacaatg gtacgaatct
     1201 cttccacagc tttggtgatt tcagcgtcgg ggttcaggac gtcgcgcgct ttgtgaatga
     1261 tgcgcacctt cccaccacaa atattctcag tcgtgtgacc ggcggaaatc cctcgaatat
     1321 cttcggcgag atcaatacga cgaactttcc cggcgcgaac ctctatctca tcaacccggc
     1381 cggcgtgctg ttcggcgcca ctgccacgtt aaatgtgagt ggatcagtgc acgtctcgac
     1441 agcggattac ctgcgcttag ccgatggcct gcgcttcaat gccatcccag gtcctcagga
     1501 tacgcttctc tcacaggcac cggtagtcgc cttcggattt ctgcatgccc atccccgtcc
     1561 aatcaccgtc caaggcagcc ggctctcagt gggagagggc aaggtcctct cgctggtcgg
     1621 gggcgacatt aaagtcaccg gcggaacact cacagcttcc agcgggcaga ttaacttggt
     1681 gagtctcgct ggtaaaggtg aagccagggt agctccggat ggcattgcga tcgctggaat
     1741 gaccccgcga ggccagatcg tgatcaagga gcggttcccc cgcgagaacc acgctcctgt
     1801 cgcagggctc gatacgtcgg gggatgaagg cggtgctgtc gttattcgcg gagggcggtt
     1861 gtcgctaaca ggagctgagg tcatcgctga tgcgagggtg gccaatcaca cgggtcgagg
     1921 cattgtgatc gaggcgacgg actccgccac aatggacaac gtgagcttgc gtacaagtgc
     1981 gttcggtgtt cccaacccga acactggggg tggggcaggg cccatttcgg tgacggctcc
     2041 ttcgatgaca gcatcaaact cgtctattag tacgtcatcg ggaaacaatg gccccgccgg
     2101 ggatgtagcc atcaatgttg gaagcctcac cgcgtcaaat ctacagatag aaaccggcat
     2161 aggcagtggt aatcgccctg ctcctgcaag cgggaacatt tccattcgtg cgactggaga
     2221 tgtcacactt cagaatagcg gcatttctac cgggacattc ggcctgcggg atgcagggca
     2281 cattactgta cgggcggcca cgctggctct gacggaaggc tcatcaatta gttctggttc
     2341 tcagttcgac gggtcgtcgg gcggtggccg cgcggggaat attgccattg acgtcgaaca
     2401 tttaaccatg cgcacatcgt cgtctattaa cacat
//
LOCUS       DATMTI010001022         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_209573, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001022 DATMTI010000000
VERSION     DATMTI010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209573"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VN039_16160"
     CDS             complement(<1..1326)
                     /locus_tag="VN039_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWV47531.1"
                     /translation="MSDTVTLIISFGGGLALGGLLVGLWITSRLRPQAQRAEATVEEL
                     RKQVELERDALMSLRQELSVVQQARVTAETRMEDTARHLTEQKNLLDHTRQELMGSFQ
                     ALSGEALKQNNEAFLKLAAVSFETLHVKADGDLVQRQQAIDALVRPLQESLQRYDEQL
                     RLLEQSRQSAYGGLDQHLKLLAESQQRLQQETGNLVKALRAPTIRGQWGELTLKRVAE
                     LAGMVDHCDFVEQPSTTGDDGRSRPDMVVRLPGGRQIIVDAKAVLSAYLEAHEAQNEA
                     QQMEALRRHAAQVKCRMDELSLKAYWTQFDRAPEFVVLFLPGEQFLGAALDQNPRLIE
                     EGFANGIVLATPATLIALLRAVAYGWRQERMTSHAEEAGRLGKELYERMAVLAEHMND
                     VGQALSKSVSAYNRAVGSLETRILPAARRFKELGVSSDRDIPVLGPTEVV"
     gene            complement(1460..>1553)
                     /locus_tag="VN039_16165"
     CDS             complement(1460..>1553)
                     /locus_tag="VN039_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HWV47532.1"
                     /translation="HYRAIATRYDKTARNFLSAIYLAAAVMWLN"
BASE COUNT          348 a          450 c          412 g          343 t
ORIGIN      
        1 aacaacttcc gtgggcccca acaccgggat gtcgcgatcc gaggataccc ccaattcttt
       61 gaagcgccgt gctgcgggga gaatgcgtgt ctcaagagaa ccgactgccc ggttgtaagc
      121 agacacgctt ttactgaggg cctggcccac atcgttcata tgttccgcca gcacagccat
      181 tcgctcatac aattctttgc ccaatcgacc ggcttcttct gcatgggatg tcatccgctc
      241 ttggcgccag ccgtaagcga ccgcccggag caatgctatg agcgtcgccg gggtcgccaa
      301 cacgattccg tttgcaaacc cctcttcaat aagacgggga ttctggtcca gcgccgctcc
      361 gaggaattgc tcacctggga gaaacaagac gacaaactcc ggcgcacggt cgaactgagt
      421 ccagtacgct tttagagata gttcatccat gcggcatttg acctgagcgg cgtgacgacg
      481 caaggcctcc atttgttggg cctcgttctg ggcttcatgg gcctcgaggt aagccgagag
      541 cacggccttc gcatctacga taatttgtcg tccaccaggc aaccggacga ccatatccgg
      601 acgggaacgc ccatcatcgc cggtcgtaga gggttgctcg acaaaatcgc agtgatcgac
      661 catccccgcg agttcagcga ctcgcttcaa cgtcaattct ccccattgac ctcgtatggt
      721 cggagcccgc aaggctttca ccaggttccc cgtctcctgc tgcaaccgtt gctgagactc
      781 cgctaacaat ttcagatgtt gatccaaccc accgtaggcc gactgacgcg actgttccaa
      841 cagacgcaat tgctcatcat agcgttgaag cgattcttga agcgggcgaa ccaaggcatc
      901 gatagcctgt tgacgctgca ccaagtcacc gtccgctttg acatgaagag tttcgaacga
      961 cacggcggcg agcttcaaga acgcttcgtt gttttgtttc aatgcttccc cagaaagggc
     1021 ctggaacgat cccataagtt cctggcgcgt atggtcaagc agatttttct gttcggtgag
     1081 gtgccgcgca gtgtcttcca tgcgggtttc cgcagtgacg cgggcttgtt gaaccacaga
     1141 caattcttgg cgaagggaca tgagggcatc tcgttccagt tctacctgct ttcgcagctc
     1201 ttccacagta gcctctgctc gctgggcctg gggacgcaag cgacttgtga tccagagtcc
     1261 gaccagcagg ccgcccaagg caagtcctcc cccgaagctt ataataagag taactgtgtc
     1321 agacatactt gacccagcac gaacttctat atatagcgtt agagagccga aattctctgc
     1381 agggtacgca atggattgag aaaggtcaag gaagcagaag gccccaatcg actagctcta
     1441 tctaccctag ggcgtgtcgt cagttaagcc acatgacggc ggcggcaaga taaatagcag
     1501 agagaaagtt acgtgccgtt ttgtcgtagc gtgtggcgat cgccctatag tgc
//
LOCUS       DATMTI010001023         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_675258, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001023 DATMTI010000000
VERSION     DATMTI010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675258"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VN039_16170"
     CDS             complement(<1..712)
                     /locus_tag="VN039_16170"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HWV47533.1"
                     /translation="MSGWKTIPLGEFAESVDYGVTASAAEQPVGPKFLRITDIQDGGV
                     NWENVPWCECDVRLAAAARLKAGDIVFARTGATTGKSFLISECPTDAVFASYLIRVRV
                     GNTAEPRFVSHYFQTPHYWAQVTKSARGAAQPGINATTLKSLKIPLPPLEEQRRIAEI
                     LDKADALRAKRRAALAKLDTLTQSIFLDMFGDPVTNPKGWPKKPFGQVCETRLGKMLD
                     QKQQTGKHRRPYLRNANVQ"
     gene            complement(709..2262)
                     /locus_tag="VN039_16175"
     CDS             complement(709..2262)
                     /locus_tag="VN039_16175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HWV47534.1"
                     /translation="MITGDIKNQIDRIWDSFWSGGISNPLEVIEQITYLLFIRRLDDL
                     HQLEENRANRLKQSMERRIFPAGNDSRGRSYEDFRWSRFKHFAPAEMFTVVGEHLFPF
                     LRADLARQLGNGDSTYAHHMKDARFTIPTAALLAKVVDMLDHVPMEERDTKGDVYEYM
                     LAKIATAGQNGQFRTPRHIIRLMVEVTAPSPTDVICDPACGTAGFLVAAGEYLRERRP
                     NLLHDTKQRAHFHHLMFHGFDFDNTMLRIGSMNMLLHGVESPDIRYRDSLAQDHVGEE
                     EKYTLVLANPPFAGSLDYENTAKDLLQIVKTKKTELLFLALFLRLLKPGGRAAVIVPD
                     GVLFGSSKAHKELRRILVEDQKLDAVIKLPGGVFKPYAGVSTAILVFTKTNSGSTDHV
                     WFYDVGADGWSLDDKRTPLLSEDKLGPTPRTPLTEDEHAKNNLPDILARWTQRTGTER
                     ERPRTAQSFCVPKTDIATQGYDLSLNRYKEVVHEAVEHRPPKEILAELAKLEEEIQRG
                     MKELEGMLG"
     gene            2502..2918
                     /locus_tag="VN039_16180"
     CDS             2502..2918
                     /locus_tag="VN039_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47535.1"
                     /translation="MSLRNLFRLIVAVSVYGTVGQVWAYDASPNISSGVDACVNVIFS
                     AFTPPPFSVDKNNVTVAPKSEFSFLTSKTALFPSITVKIKEEKVPVTVTPINNGFLVK
                     GKLPPTIKGSYIRLDIFAKGPNDCDKTDGWLLKVGN"
BASE COUNT          635 a          920 c          871 g          722 t
ORIGIN      
        1 actgaacatt cgcattgcgt agatatggtc tccgatgctt gccagtctgt tgcttctgat
       61 caagcatctt cccgagtctt gtctcacaca cctgtccgaa tggcttcttt ggccatccct
      121 tcggattcgt aaccgggtcg ccaaacatgt cgaggaagat ggattgggtg agggtgtcga
      181 gcttcgcgag ggcggcgcgg cgcttggccc gcagcgcatc cgccttgtcc agaatctccg
      241 caatccgccg ctgctcctcc agaggcggaa gtggaatctt gagagacttc aacgtggtcg
      301 cgttgatgcc aggttgggca gcacctcgcg cgctcttcgt cacctgtgcc cagtagtgcg
      361 gtgtttggaa gtaatggctc acgaaacgcg gttcggcagt attgccaaca cgaacgcgaa
      421 tcagatacga cgcaaacacg gcatcagttg gacactcgct aatgagaaag ctctttccag
      481 tggtggcgcc agttcgcgca aacacaatat ctcccgcctt gagccgcgcg gcggccgcta
      541 agcgtacatc acattcgcac cacggaacat tctcccaatt aacaccccca tcttggatgt
      601 ctgtaatgcg aaggaacttt gggccgacgg gctgctcagc ggcagacgcc gttacaccat
      661 aatcaacaga ctcggcaaac tcaccgagtg gtatggtctt ccatccactc atcccagcat
      721 cccctctagt tccttcatcc cccgctggat ctcctcttcc agcttcgcca gctcagcgag
      781 gatctctttc ggcgggcgat gctcgaccgc ctcgtgtaca acttccttgt aacggttgag
      841 cgagaggtcg tacccttgcg tggcgatgtc ggtcttcggc acgcagaaac tctgcgcggt
      901 gcgtgggcgc tctcgctcgg ttccggtacg ttgagtccag cgcgcgagga tgtcaggaag
      961 attattcttt gcatgctcgt cttcagtgag cggcgtgcgg ggcgtgggtc caagtttgtc
     1021 ttcagacagc aacggcgtcc gcttgtcgtc gaggctccag ccatcggccc cgacgtcgta
     1081 gaaccagacg tgatcagtgc tgcccgagtt cgtcttggta aagacaagaa tcgcggtgga
     1141 gacgcctgca tagggcttga acacaccgcc aggcagcttg atcactgcat cgagcttttg
     1201 atcctccacc aagatgcggc gcagctcctt gtgagcctta cttgagccga agagcacgcc
     1261 atccggcacg atgactgccg ctctcccccc aggtttcaag agacgcaaga agagcgcgag
     1321 gaagagcagc tcggtctttt tggttttgac gatctggagg agatccttgg cggtgttctc
     1381 gtaatcgagg ctcccggcaa acggtggatt ggcgagcacc agcgtatact tttcctcctc
     1441 gcccacgtgg tcttgcgcga gcgagtcgcg gtagcggatg tccgggctct ccaccccgtg
     1501 caacagcatg ttcatgctgc cgatgcgcag catggtgttg tcgaagtcaa agccgtggaa
     1561 catcagatgg tggaagtgcg ctcgctgctt cgtatcatgc agcaagtttg ggcgtcgctc
     1621 gcgcaagtac tcgcccgctg ccaccagaaa gcccgccgtg ccgcaggccg gatcgcagat
     1681 aacatctgta ggcgatggcg cagtcacctc aaccatcagc cggatgatgt gacgcggggt
     1741 gcggaactgc ccgttctgcc cggcggtggc gatcttggcc agcatgtact cgtagacatc
     1801 gcctttggtg tcgcgctcct ccatcggcac atgatcgagc atgtccacca ccttcgccaa
     1861 cagcgccgcc gtcgggatgg tgaaccgcgc gtccttcatg tggtgggcgt aggtggagtc
     1921 cccgttgccc agttggcgcg caagatcggc acgaaggaac gggaacagat gctcgccgac
     1981 gacggtgaac atctcggccg gggcgaagtg tttgaagcgc gaccaacgga agtcttcgta
     2041 cgaacggccc ctggagtcat tccctgccgg gaagatccgc cgctccatcg actgcttgag
     2101 ccgattggcc ctgttctcct ccagctggtg taagtcgtcg agtcggcgga tgaacagcag
     2161 gtaggtgatc tgttcgatca cctccaacgg attcgatatg ccgccggacc agaacgagtc
     2221 ccaaatgcgg tcgatctggt tcttgatgtc accggtgatc atgcggcctt ccttttatga
     2281 acctcgtacg ttctcttccc cttgttgaac aagaggaata attagcggag aggcataccg
     2341 cctttttata agaaaggcaa tgattccgaa ggtagtaatc gaccggccca tacgaggccg
     2401 cctcagattt ttggggttgt tttttctatg tgaggcgtac aaaataccgc cgcgttattc
     2461 atgctggtcc tatcatccac cctcactaga gaggcataac cgtgagtctg cgaaatcttt
     2521 tccggcttat tgttgcagtg agtgtgtacg gtaccgtcgg ccaagtctgg gcgtatgacg
     2581 cgagccctaa tatcagtagc ggcgtggacg cctgcgtcaa cgtcatcttt tctgccttca
     2641 cgcctcctcc cttctccgtg gataagaata atgtcacggt ggcgccgaaa tcggagtttt
     2701 cattcctgac ctccaagacc gctctttttc caagcatcac ggtgaagatc aaggaggaaa
     2761 aagttccggt caccgtcact ccgatcaata acggcttcct ggtcaaaggg aaactgcccc
     2821 ccaccatcaa aggcagctac atccggctcg atatttttgc caaaggcccc aacgactgtg
     2881 acaagaccga tggttggctg ctgaaagtgg gcaactgact gtgcgcgaga cgatccgggc
     2941 aggggacttc ccctgcccgg tcgttatccc gacactcgcc tcaatcatcc ttagggctct
     3001 tcctcttcga gagactcaat cgattccttc agcgcattta tcatgagtct aaggcggtct
     3061 atgacaacgt gccgggacaa tccacgtcat ttgatcctat tatgcggtct catctcagtt
     3121 ttgttgacga acttgcatca ccgacgat
//
LOCUS       DATMTI010001024         3817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_548724, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001024 DATMTI010000000
VERSION     DATMTI010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548724"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..476
                     /locus_tag="VN039_16185"
     CDS             18..476
                     /locus_tag="VN039_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWV47536.1"
                     /translation="MSIGILLPLTGVMSITLAISSVTFAQGLPPIVKQKVEAAQKQVK
                     TIGIEEYGKLVENPGAALIVDVREPHEYAAGHVPGAINIPRGLLEFKIWSHVGFPANG
                     EMDRPLILQCQSGSRASLAAQSLQGLGFTQTTAVVMNLEDWQKAGRPFTK"
     gene            550..852
                     /locus_tag="VN039_16190"
     CDS             550..852
                     /locus_tag="VN039_16190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWV47537.1"
                     /translation="MIPDASLRVVARVRAKTEHVAQVREILSALVEPTRRESGCLSYE
                     LLQNSSDPADFVFVEKWASAATEHAHFATPHVSVALQRLVGLLAAEPEICRYKILK"
     gene            857..1915
                     /locus_tag="VN039_16195"
     CDS             857..1915
                     /locus_tag="VN039_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47538.1"
                     /translation="MNEILFCPASLKPTPSPSIATVLYCTTMMPVTPDTELSISFENQ
                     RAIISPWGASLRRYLLINDGGQETNIVWGYSGGSGKRGGQGDVLIPFPGRISYGRYTF
                     EGQLLQLERNDKEGPNAIHGFVRSLPWHVQESHSNKVTFEIRLDADTYAEQGYPFSLA
                     VWVTYELGASGLACTFTVKNVGQQAAPVGAGFHPYFTVGTSSIDEAEVQIPGTAYLEF
                     NERLVPTGTILSVIGTPWDYRRFHAVGSQRFNHCYVQLERDAQNMATVSLRHTARNRR
                     IDITMDHTFSAVVVYTGDAIADAPRVALAIEPMTCASDAFNHPDWGLKRLVPGETFSG
                     RYSVRYRVIGKDSLCASA"
     gene            complement(2025..2183)
                     /locus_tag="VN039_16200"
     CDS             complement(2025..2183)
                     /locus_tag="VN039_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47539.1"
                     /translation="MIWQDQGKWRGCLQASPDQQVQGESFEELQLKLLQLHRDLISGR
                     PSNLRKIA"
     gene            2267..3184
                     /locus_tag="VN039_16205"
     CDS             2267..3184
                     /locus_tag="VN039_16205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV47540.1"
                     /translation="MRTHTRMVLNTADAHAVTFQQISANQLSFRVATAGSGDRLILCL
                     HGFPESAVSWRYQIVPLAQAGFLVWAPDLRGYAGTTRPAGIDAYRIESLMDDVTGLLD
                     AAQVQRAILVGHDWGGIIAWYYAMRHADRVETLVIVNAPHPACFEREVRHWRQWHRSW
                     YMGFFQIPWLPEAALSACHGYVIGEIFGRMTIHCEQMPHDLIQLYRRQACEPGALTAM
                     VNYYRAALRGGGAMRQRRLGYPAISVPTLVIWGLQDHALVRHNLDGLNDFVTDLTVVK
                     VADAGHFVHEDKPERVTSEMLTWLDLKRV"
     gene            complement(3210..>3817)
                     /locus_tag="VN039_16210"
     CDS             complement(3210..>3817)
                     /locus_tag="VN039_16210"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWV47541.1"
                     /translation="NSGSNDVTAFSIGATGLVSRIAPSGANANPISTGGKVPKDVAIS
                     PNGSFLYVANSGSDNLTVFQIGTNGLLTLVPPAGSNPNPVSTGGTTPNALMISLDGRF
                     LYVANGGGNVSAFTIGGDGLLTLVLNSAGNLNPVSAGTNPIALTMSPDGRFLYVANQG
                     GRVSAYTVNTETGPLTPLTALVGNPFPTGTTPSAIATPGRP"
BASE COUNT          811 a         1024 c         1119 g          863 t
ORIGIN      
        1 cttgaggaga agactacatg agcataggga tactattacc gttgaccggt gtcatgagca
       61 taaccctagc catttcgtca gtgacattcg ctcaaggttt gcctcctatc gtgaagcaga
      121 aagtcgaagc ggcgcagaag caggtgaaga caatcgggat agaagagtat gggaagctcg
      181 ttgagaatcc aggtgctgct ttgattgtgg acgtacggga gccgcatgag tacgccgcag
      241 ggcatgtacc tggagcgatc aatattcccc gcggtctgct cgagttcaaa atctggagcc
      301 acgtcggctt tccggccaac ggcgagatgg accgccctct cattctgcaa tgtcaaagcg
      361 gcagtcgcgc ctcacttgcg gcacagtcgc ttcaaggtct ggggttcacg caaacgacag
      421 cagtcgtcat gaacctcgag gactggcaga aggccggccg tccattcaca aagtagccta
      481 cccgcatccc ggagcacatt cgcctctctt tcagacgagc atgtatcgcc ttatgctatg
      541 gagcacgaca tgatcccaga cgcttccctc cgagtggttg cgcgtgtcag ggctaagact
      601 gagcacgtcg cacaggttcg ggaaatctta tctgcgcttg ttgaaccgac ccgccgcgaa
      661 tcgggatgcc tcagttatga gcttctgcag aatagttccg acccagccga ctttgtgttc
      721 gtcgagaaat gggcgagcgc ggcgacggaa catgcgcatt tcgccactcc acacgtctct
      781 gttgcactgc aacggttggt aggacttctg gccgccgagc cggagatctg tcggtacaag
      841 atcttaaagt gacgtggtga atgagattct gttctgtcca gcttcgctca aacctacgcc
      901 ttcgccatcc attgcgacgg tgctatactg cacgaccatg atgccggtca cacctgatac
      961 tgaactctcc atatcctttg agaaccagcg tgcgattatt tctccctggg gagcttcgtt
     1021 acgacggtac ctgctgatca acgacggcgg gcaggaaacg aatattgtgt ggggatattc
     1081 gggtggcagc gggaagcgag gcggacaggg cgacgtgctc attccatttc ctggccgtat
     1141 cagctatgga cgttatacgt ttgagggaca gttgctgcag ctcgaacgta acgacaagga
     1201 aggtccgaat gccattcatg gattcgttcg cagtctgccg tggcacgtgc aggaatccca
     1261 ttcaaacaag gtgacgtttg agattcgtct cgatgctgat acatatgctg aacaaggcta
     1321 tcccttttca cttgccgttt gggtgacgta tgaactcggc gcctccgggc tagcctgcac
     1381 attcaccgtc aagaacgttg ggcaacaggc agcgccggtc ggagccggat ttcatcctta
     1441 cttcaccgtt ggaacgtctt caatcgacga ggccgaggtg cagattccgg gaaccgccta
     1501 tttagaattc aacgagcggc tcgttccgac aggaacgatt ctgagcgtga tcggcacacc
     1561 gtgggactat cgacgatttc atgcggtcgg cagtcagcgc ttcaaccatt gttatgtgca
     1621 gctcgaacgt gatgcacaga acatggccac agtatctctg cgtcataccg cgcgcaatcg
     1681 aaggatcgac attacgatgg atcacacgtt ttccgcagtg gtcgtgtata ccggggatgc
     1741 tattgcagac gcgccacgag tcgcgcttgc catcgaaccg atgacctgcg cgagtgatgc
     1801 gttcaatcat ccggactggg ggttgaagcg gctcgttccc ggtgagacat tctctggacg
     1861 ttatagcgtg cggtatcgag tcataggaaa ggattctctg tgcgcctcgg cttgagcatc
     1921 tgctttggct atggctgaca ctcgtcggat gcgtcggacg agcgagtccg acgacagctt
     1981 gcactgtcgg ctagggatct cggtgtctca gcggggacta tcgctcaggc aatcttccgg
     2041 aggttggaag gcctgccgct gataagatcg cggtgaagct ggagaagctt gagctgcagc
     2101 tcttcgaacg attccccttg aacctgctgg tcgggagaag cctgcagaca gccgcgccac
     2161 ttgccctgat cttgccaaat catgtatttc gtcatgtcac ggagtatagc tgcaagtcgg
     2221 agttccgaac agaattttgt aagtttttac ccaggcgcgc atcgaaatga gaacgcatac
     2281 gcgcatggtt ctcaatactg ctgacgcaca cgctgtgacg tttcaacaga tctccgcgaa
     2341 ccaactgtct tttcgagtgg caacggccgg gtccggcgat cgattgatcc tctgtctaca
     2401 cgggtttcca gaatccgccg tgtcatggcg gtatcagatc gttccgctgg cgcaagcagg
     2461 atttttggtt tgggcccctg accttcgggg atatgccggc acgacacggc cggcaggcat
     2521 tgacgcctac cgaatcgaat ccttgatgga cgatgtcacc ggtctcctcg atgccgcgca
     2581 ggtgcaacgc gccattctgg tcgggcatga ttggggcgga atcatcgcct ggtactatgc
     2641 gatgcggcac gccgatcggg tggaaacctt ggtaattgtg aacgctcctc acccggcctg
     2701 ctttgagcgg gaagtgcggc actggcgtca gtggcatcgt tcctggtaca tgggcttttt
     2761 ccaaattccc tggcttccgg aagcagcttt gtcggcctgt catggctatg tgatcggtga
     2821 gatctttggg cgcatgacga ttcattgcga gcagatgcct cacgatctca ttcagttata
     2881 tcgacgacag gcctgcgagc cgggagcgct cacggccatg gtgaattact atcgcgcggc
     2941 cttacgaggc ggcggggcga tgcgtcagcg aaggttggga tatcctgcta tctccgtccc
     3001 cacactggtg atctggggat tacaggatca tgcactcgtc cgtcacaatc tcgatgggct
     3061 gaatgatttc gtcaccgatc tgacggtggt aaaagtcgcg gatgccggac acttcgtgca
     3121 tgaagacaaa ccggagcggg tcacgagtga aatgctgacc tggttggatc tgaaaagagt
     3181 gtagatatag gatggcttca acgcctgacc tatggacgac cgggcgtcgc aatcgccgag
     3241 ggtgtggttc ccgtcggaaa gggattgcct acgagagccg tcaatggggt caggggccct
     3301 gtttccgtgt tgacggtata ggccgataca cgacctccct ggttcgccac gtacaggaat
     3361 cgcccatccg gtgacatcgt caaggcaatg ggatttgtcc ccgctgagac gggattgagg
     3421 ttgcccgccg agttcagcac gagcgtcaag agtccatcgc ccccgatggt gaacgccgat
     3481 acattgcctc ccccattggc gacataaaga aatcggccat ccagggagat catgagcgcg
     3541 ttgggagtcg taccgccggt ggagactgga ttcggattgg accccgcagg cggcaccaaa
     3601 gttaagaggc cgtttgttcc gatctgaaag accgtcagat tatcagagcc gctgttagcc
     3661 acataaagaa acgatccgtt cggcgagatg gccacatctt tggggacttt cccaccagtc
     3721 gagataggat tggcgttcgc cccggacgga gcgatcctgc tcacaagccc ggttgctccg
     3781 attgagaacg ccgtgacatc gttggagccg ctgttgg
//
LOCUS       DATMTI010001025         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_803376, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001025 DATMTI010000000
VERSION     DATMTI010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803376"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VN039_16215"
     CDS             complement(<1..226)
                     /locus_tag="VN039_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HWV47542.1"
                     /translation="MTDRVRIGILATVLLSADLSMTLGANPPPTPFQSATAGYRYNFP
                     RDHGSHPTYRTEWWYYTGHLHSKSGRSFGFE"
     gene            416..1105
                     /locus_tag="VN039_16220"
     CDS             416..1105
                     /locus_tag="VN039_16220"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWV47543.1"
                     /translation="MQTDRERGSPDRSPSKHAEPFPIPSRLPVFPLPNVVFFPKTYLP
                     LHIFEPRYRRMVADVTMGGQCIAMALLKEGWEPDYYENPAIYPALCIGRIVSVQPLSD
                     GRSNILLQGLERGEISEEHFDKPYREATIRITPMRSDEGLTKGVRRALIDVLGRYLQA
                     REDSAAWQGFFREEVSDEVLVNTLSTYLDCTPLEKQFLLEADGLHQRARRLNDLVQFM
                     LHEHHDTKGWE"
     gene            1105..2076
                     /locus_tag="VN039_16225"
     CDS             1105..2076
                     /locus_tag="VN039_16225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV47544.1"
                     /translation="MDRTIAIDVSRLCKTYDSHKAVDDLSFQVYAGEIFGLLGPNGAG
                     KSTTLRTLITLLHPTSGTATVMGYDTVREADQVRTVIGYVPQERAIDRFLTGREHLQL
                     LGALYHLTKEESAKRIGELLKLVELEAHADRPAKTYSGGMKRKLDIACGLLPDPKILF
                     LDEPTLGLDVQSRLRIWEYVRMLKARGMTVVMTTNYLDEADRLCDRLAIIDGGKIKTL
                     GAPAELKIALGGDIVSLTLKETNRIPSLESDLTGRPAVKTVRATAKGLDIRVETPEKA
                     LPAILESANRLGCDIEFIQYNRPRLDDVFIAHTGRAITESPPEVESS"
     gene            2086..2865
                     /locus_tag="VN039_16230"
     CDS             2086..2865
                     /locus_tag="VN039_16230"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV47545.1"
                     /translation="MAHYWQEINALTMRWVRRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADTQVMQAPNYISFLAAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHR
                     TSVILSRFIFVMAITSMQVLVILGVSYLFGVHPATGILGVATILLIGMMFGIGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLTAMPGWMSFLAQFNPMTWAIDAVR
                     PLILSGWTEALPHVGMVVLVMLVFDALCLYGGAKAFRRAMG"
     gene            2978..>3293
                     /locus_tag="VN039_16235"
     CDS             2978..>3293
                     /locus_tag="VN039_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47546.1"
                     /translation="MIPENQIRALIRLLSDEDDRIVRTISGRLIDIGPSAVPLLQEAE
                     IEQPEMADRIASVLEEIRGSKLEDELTSLAALSDEAMSLEAGAFLIARYAYPTLDVAR
                     YHE"
BASE COUNT          696 a          936 c          934 g          727 t
ORIGIN      
        1 attcaaaacc aaacgaccga ccgctctttg aatgcagatg gccggtgtag taccaccact
       61 cggttcgata ggtcgggtga gatccatggt ctcggggaaa gttgtaccga taaccggcgg
      121 tcgccgactg aaacggtgtt ggaggagggt tcgcgccgag cgtcattgag agatcagcgc
      181 tcagcaagac agtggccaag atcccgattc gtaccctatc tgtcacactt gacctcgtat
      241 cagcagtaaa tcctgagagg atcacgtaag taggacactc ctctcgaatt gtttataaca
      301 cgcgccgtgc caatgaacca atcgacactc ttagagcgat gtttcgcatt tccccgtggc
      361 tcaacggtca gcacatagcg ttgacaattt tcacaagtct cagctatcgt gagccatgca
      421 gaccgatcgt gagcgggggt cgcctgaccg gagtccgtcc aagcatgccg agcctttccc
      481 aattcctagc cgccttccgg tattcccgtt acccaacgtc gttttttttc ccaagacgta
      541 cctgccgctc cacatttttg aaccacgcta tcgccgaatg gtcgctgatg tgacaatggg
      601 cggtcaatgc attgccatgg cgctgctgaa ggaaggatgg gagccggact actatgagaa
      661 tccggcgatc tatcccgcgc tctgtatcgg acggattgtg agtgtgcaac ccttgtcgga
      721 cggtcgctcc aacatcttgc tgcaagggct cgaacgaggc gagatctccg aagaacactt
      781 tgacaagccg tatcgcgaag cgacgattcg catcacgccc atgcgctccg acgagggctt
      841 gacgaaaggc gttcgacgcg cactgatcga cgtgctcggg cgatacctcc aagcgagaga
      901 ggatagcgcg gcgtggcaag ggttctttcg tgaagaagtg agtgacgaag tcttggtcaa
      961 caccctctcc acctaccttg actgcacacc cctggaaaaa caattcttac tggaagcgga
     1021 tggactccac caacgggctc gtcgattgaa cgatctggtt caattcatgc tgcacgaaca
     1081 tcacgatacg aagggctggg agtaatggat cgaaccatcg caatcgacgt cagccgtctg
     1141 tgcaaaacgt atgacagcca taaagccgtc gatgacctct cgttccaggt ctacgccgga
     1201 gaaatttttg gtctgctcgg gccgaacggc gcggggaaaa gcaccactct tcgcacgctc
     1261 attacgttgc tgcacccgac ctcaggcacg gcgaccgtca tgggttacga cacggtgcgc
     1321 gaggcggacc aggtccggac tgtgatcgga tacgtgcctc aagaacgggc gatcgaccgc
     1381 tttctgacgg ggcgtgaaca cttgcaactc ctcggtgcgc tctatcacct gacgaaagaa
     1441 gaatcggcga aacgcatcgg cgagttgctc aaactggtgg aactggaagc ccatgcggac
     1501 cggcccgcca agacctattc cggcggcatg aagcgcaagc tcgacattgc ctgtggcttg
     1561 ctgcctgacc ccaagatctt gtttctcgac gagcccaccc tcggcctcga cgtgcaaagc
     1621 cgtctccgga tttgggaata cgtccggatg ctcaaagccc gtggaatgac ggtcgtgatg
     1681 acgacgaact atctggacga ggccgatcga ctctgcgatc ggctcgccat tatcgacggc
     1741 ggcaagatca aaacgctggg cgccccggct gagctcaaga tcgctcttgg cggggatatc
     1801 gtgtccctca ctctaaaaga gacgaaccgg atcccgtcgc tggagtcgga ccttaccggc
     1861 cgtccggcgg tcaagaccgt gagagcgacc gccaaaggct tggatatcag agtagaaacg
     1921 cccgaaaagg ccttgcctgc cattcttgaa tcggccaatc gcttgggttg cgacatcgag
     1981 tttattcaat ataaccgtcc tcgcttggac gatgtgttta tcgcgcacac cgggagagcc
     2041 attaccgaat cgcctcccga agtcgagtcg tcgtaaagga gtcccgtggc gcactattgg
     2101 caagagatca atgcgttgac gatgcggtgg gttcggcggt tgagccggga aaaattcagc
     2161 atgctgttca ccttggtgca gccgatgctg ttctggctca tcttcttcgg caacctgttt
     2221 caacgggccg cggacacgca ggtcatgcag gcgcccaact acatcagctt ccttgcggcc
     2281 ggtgtcgtcg tcatgacggt cctgaacaac ggcttggccg gaggcgtcga tctgctcttc
     2341 gataaggaaa acgggttcct tgagcgtttg atgtccacgc cgattcatcg tacctcggtc
     2401 atcctgagcc gctttatctt cgtcatggca atcacgtcga tgcaggtcct cgtgattctc
     2461 ggcgtgtcgt acctgttcgg cgttcatccc gcgacgggta ttctcggtgt ggcgacaatc
     2521 ttgctgatcg ggatgatgtt tgggatcggc ctgacggcca tctccatggc aatggccttc
     2581 tccgtgaaaa gtcacgggga cttcttctcg gtgttgggat ttctttcgct gccgatgatc
     2641 ttcctcagct ccgcattggt gccgttgacg gcgatgccgg gctggatgag tttcctggcg
     2701 caattcaacc cgatgacctg ggcgatcgat gcggtgcggc ctttaattct gtccggctgg
     2761 actgaggcct tgccgcacgt aggaatggtg gtcttggtca tgctcgtgtt cgatgccctg
     2821 tgtctgtacg gcggggccaa ggccttccgc cgtgcaatgg ggtaacctca ccatggaacc
     2881 cacaacccat ggatcgcttg actgacggcg gaatccgtcg gatcatgatc ttaatgccgc
     2941 catacgtcgg tctgtgaacg acggaaagtc gtaggagatg attcctgaaa atcaaattcg
     3001 tgcgcttatt cgcctcctgt ccgatgagga cgaccgaatt gtccgtacca tcagcgggcg
     3061 tctcatcgat atcggtccat cggctgtccc gcttctccaa gaagcggaaa ttgagcagcc
     3121 ggagatggct gatcgcatcg cttccgtgct cgaggaaatc cgaggaagca agctggagga
     3181 tgaactgaca agcctggcag ccctctcgga cgaagccatg agcctggaag ccggcgcctt
     3241 cttgatcgct cgctatgcct atccgacgct cgatgtcgct cgctatcatg aac
//
LOCUS       DATMTI010001026         5983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_336226, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001026 DATMTI010000000
VERSION     DATMTI010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336226"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1411)
                     /locus_tag="VN039_16240"
     CDS             complement(113..1411)
                     /locus_tag="VN039_16240"
                     /inference="COORDINATES: protein motif:HMM:NF024144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HWV47547.1"
                     /translation="MRWNRICLGQRFITVLLLAIGLNAYGCKDSVSVSEDVQVPLASL
                     TVTPGTLQPGFFSNTTNYTVNAPTSATSVTVTAIPKDNTATVTINKAVTTHLSVTLGG
                     PGSTTIITIVLEAQNGAESTYTVNVTRVLSSDNNLSALDVTPGNLAPAFSPDTENYIV
                     NVGVLVDSVTVSATKSDPTAMMLIGPTPVPPGTASGQTTIQLGGPGTATPVTITVTAP
                     NGTAKTYTITVNRAALSGNNNLSALTVTPGTLVPSFNPGALDYTVDVGIDVTEVTVTA
                     TKSDSNAVISGDVPNEGQAIIQLDGPGSSRLVSIIVTAPNGNSKTYTITVNRAAPSSD
                     NNLSALTVTGGPLVPDFTPSTTTYTVDVPSSVDTVTVSAIKADPNAVMSGDVTAGTGI
                     ATGQATFSLVPLFPRSVSITVTAPNGDSKIYSITITRALF"
     gene            complement(1614..2993)
                     /locus_tag="VN039_16245"
     CDS             complement(1614..2993)
                     /locus_tag="VN039_16245"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWV47548.1"
                     /translation="MRYWPTFLMLVILAGLGGYLYLVELPAEQREEKQESAQKQILSF
                     PETAITGLSITTPQGPIAFKLAEPGKWSIVAPLQTDADNREVQALIRALVTGAVARTV
                     EEQATQLAPFGLEKPVTTLTITAGTQQETISIGDSGPLSNTLYVLRASDRRVLLTNLA
                     PKDFINKSLMTYRRKELLRFVQNDVERVRLTYPTTEIVIYNMAKDKPRPSWKIRYPIE
                     AEGDQNEIRSLMFRLEDLKALGIIDPGPERDTVGKTLTIPKVKVTLHTAAGDQSVRLY
                     QPDPQSGEAIAETTADAPLYRVNPALIKDLTKELFNLQDKRLLGLDYTDIAMLSVKTR
                     DHQYVLINQTGEWVLEDRPNEKVSQEAADLFVSRVANLPAEERVMKQSAPLAPYGLLA
                     PAAEFVATGKDGKTSGKLTLGNQAGNLLYATGQRLQGVFQVRPDLLTQIPSKTDLLAK
                     SQEKTPAGR"
     gene            complement(3234..4757)
                     /locus_tag="VN039_16250"
     CDS             complement(3234..4757)
                     /locus_tag="VN039_16250"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HWV47549.1"
                     /translation="MNLKSFPLGILGVVLGIGGMITYSLRPDWLWVATIAEGLALVCL
                     VLFFVLHFETVKAFSSRRSTRMGLNSFLMIVLFSGILVIVNFLASRHSVRWDLSENQN
                     FTLAPQTHRVLRTLPHDVKITVFTREKDPGYQPYKERLESYRQASTKLSVEFIDPEKQ
                     PKIAQSYGIFRTDTAIFESNGQTIRVTSPSEVELTGALIRISKDAKKRIVFVEGHSEL
                     SMEDKDRNGLSIAKESLTRQGYDVGTLSLLKESAVPENTSVLVLAGPRRSVTKEEQER
                     IQAYVEKGGHLLVLADPDTQTGLEPMLAHWGLGLGPGVLVDLQDRLAQGDLTALLVRT
                     FTEHEITQDLTSAVLLPLSRHVTFDEQVGKDWDFVPLARTSPNSWAETNMQGRVVSLS
                     EKEDVKGPLPMAAALAPKKAPEEGKPRPAVVVIGNSTFVSNAFFNFPGNSDFFLHTIG
                     WLAEERDLISIAPKEPALHPFTPNPTQERALIYIQVVFLPLVTLLTGIVVWRKRRRL"
     gene            complement(4754..5524)
                     /locus_tag="VN039_16255"
     CDS             complement(4754..5524)
                     /locus_tag="VN039_16255"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWV47550.1"
                     /translation="MTPVQAVIAKELRGYFVSPIVYVVGAVFLLIFGLLSYLYVGFAG
                     AQAIQLMQMQGGMAQINLNDLVFRNLFASMRIVLVIILPILTMRLFAEERKLRTFEFL
                     MTSPIRINEIVVGKFVSVFLIYLGLVGLTTLVPTVLMLFSDFDWNPIWTGYLGMVLLG
                     ALFLAVGLFASALTENQIVAAFVSFGMLLTIWLIAGLGSLFGDTTAGRVISYFSYMEH
                     YDRLVRGLIDTSDLIYFGSGLALMLFLTHRVVESTRWK"
     gene            complement(5532..>5983)
                     /locus_tag="VN039_16260"
     CDS             complement(5532..>5983)
                     /locus_tag="VN039_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV47551.1"
                     /translation="AGSHSVILSTHILPEATAVCQRVVIINKGRIVAEDTPEQLSARL
                     RQSEKVSITLKTCPSDCETKLRSIHGILNVLPGQAGGSFLIECELGRDLRDEIARVAV
                     MSQWGLLELRTISMTLEDVFLQLTRHEGHLSEPVEATVSASAERTDE"
BASE COUNT         1277 a         1594 c         1775 g         1337 t
ORIGIN      
        1 ttcggaaact gtggccgatg cctctgcacc gacgccatcc cagactgatc aagcagcacc
       61 caggcagtcc tcagaatctg tgggcaatgt atggaatgac caattgtatt ttttagaata
      121 atgctcgggt gatggtaatc gagtatatct ttgaatctcc attcggtgcc gtgacagtga
      181 tcgataccga tctaggaaat agtggtacaa gtgagaaggt tgcttgaccg gttgcgatac
      241 cggtccccgc cgttacgtca cccgacatta cggcattcgg atcggcctta attgccgaga
      301 cagtcacagt gtcgacactg gacggaacgt caaccgtata agtcgtggta ctgggagtaa
      361 agtcaggaac caacggtcct cccgtcacgg ttaatgccga caggttattg tcactcgatg
      421 gcgccgctcg gttgacagtg atggtgtagg tcttggagtt tccatttggg gcggtcacga
      481 tgatagatac aagtctgctg ctccctgggc cgtccagttg aatgatcgct tgtccttcgt
      541 tcgggacatc tccagatatc acagcattcg aatcggactt ggttgcagtg acggtcactt
      601 ctgtgacgtc gatgccaacg tccaccgtat aatccagggc tcctggatta aaggaaggaa
      661 ccaaggtacc tggtgtcaca gttaatgccg acaaattgtt gttaccggag agcgccgctc
      721 ggttcacggt gatggtgtag gtctttgcag tgccatttgg tgcagtcaca gtaatcgtca
      781 caggggtggc tgtcccaggc ccaccgagct gaatggtggt ttgccctgat gcggttcctg
      841 gtggcacagg cgtcggaccg atcagcatca tggcggttgg atcggatttg gtagcagaga
      901 cagtgacact gtcgacgaga acgccgacgt tgactatgta gttctcagtg tccggagaaa
      961 aggccggggc caagttgccc ggtgtcacat ctaaagccga cagattgttg tcgctcgata
     1021 agactctcgt gacattgacg gtataggtgc tctccgctcc gttttgtgct tccaagacga
     1081 tggtgatgat cgtggtcgat cccggtccac cgagggttac ggacaggtga gtggttacgg
     1141 ccttattgat cgttaccgtt gccgtattgt ccttggggat ggctgtcacg gtgacactgg
     1201 tcgcggatgt cggcgcattg actgtatagt tcgtggtgtt gctgaaaaac cctggctgaa
     1261 gtgtgccggg ggtgaccgtt aagctagcga gcggtacttg tacgtcttcc gaaaccgata
     1321 cggagtcctt gcagccatag gcattcaagc cgattgcgag gagaaggacg gtgatgaacc
     1381 gttgtccaag acagatacga ttccatctca tataaatccg gtcactttct gttgatgtta
     1441 ggagtgaaga accggttatt cttatgaacc cgaaccgtgg acgccgatac tacgagaggg
     1501 gtcccgatcg gtctatccct acctgcgtaa gggcgagccg atgctgggaa ggtgtgcccc
     1561 actttccggg tgggggccga ggctcagatc aagaattgag tcgatattga agatcagcgc
     1621 cctgcaggag ttttttcttg agacttggcg aggagatcgg tcttggacgg aatctgcgtg
     1681 aggagatccg ggcgcacttg aaagacacct tgcagacgtt ggccggtggc ataaagcaga
     1741 ttgcctgcct ggttgccgag ggtcagtttc cctgaggtct tgccgtcctt tccggtcgcg
     1801 acaaattctg cagcgggggc caacagccca tagggggcga gcggtgcgga ttgtttcatg
     1861 acgcgctctt cggccggaag attcgcgaca cgactgacaa agagatccgc cgcttcttgg
     1921 ctgaccttct cgttgggccg gtcttcgagc acccactcgc cggtctggtt gatcaagaca
     1981 tactgatgat cccgcgtctt caccgacaac atggcaatgt cggtgtagtc gaggcccagt
     2041 aaccgcttgt cctggaggtt gaaaagttct ttcgtgagat ctttgatcag cgccgggttg
     2101 acgcgataga gcggggcgtc ggcggtcgtc tccgcaatgg cttccccact ctgagggtcc
     2161 ggttgataca gccggacgga ttggtcaccg gctgcggtat gcagcgtgac tttcactttt
     2221 gggatcgtca gggtcttgcc tacggtgtct cgttcaggac cgggatcgat gatgccgagc
     2281 gccttcagat cttccaatcg aaacatcaac gagcggattt cgttctggtc tccttcggct
     2341 tcgatcggat agcggatctt ccatgaaggc ctgggcttat ctttcgccat attatagatc
     2401 acgatttcgg tcgtcgggta agtcaggcgg acccgttcga catcattctg cacaaaccgc
     2461 aagagctctt tgcggcggta tgtcatcagc gacttgttga tgaagtcttt cggtgccagg
     2521 ttggtcagca acacccgccg gtccgatgcc cgaagcacat agagcgtatt ggaaagagga
     2581 ccgctgtcgc cgatggaaat cgtctcttgt tgcgttccgg ctgtgatggt gagggtggtc
     2641 accggcttct caagaccgaa tggcgcgagc tgcgtggcct gttcctcgac ggttctggcg
     2701 acggctcctg tcacgagcgc tcgaataagc gcctgcacct ctcgattatc ggcgtcggtt
     2761 tgaagcggag ccacgataga ccatttaccc ggttctgcca gcttaaatgc gatgggtccc
     2821 tgaggggtgg tgatcgagag tccggtaatc gcggtttccg gaaaagaaag gatctgtttc
     2881 tgggcgcttt cctgtttctc ctctcgctgc tcggcgggaa gctccacaag gtagagatag
     2941 ccgcccagac cagcgaggat caccaacatt agaaatgtgg gccagtagcg catatcagga
     3001 tggtgaaaat ggccgccagc tgtgttctcg catcgttcag accctcaacg tatcggacac
     3061 ggccggctca ctgtctcgcc ggcgcgcaca gacttggcgc tccttattcg tcgcgccgtg
     3121 cgcctcgcgg cggccttgcc cgcggaacag cgagtcttga ctcgctgggg cagggcgggt
     3181 gaaataaaat agccattttg accatcctgc agaagatatg taaggaaggc ccatcataat
     3241 cggcggcgtt ttctccacac gacgatgccg gtgagcaacg tcacgagcgg gaggaacacg
     3301 acctgtatgt aaatcagtgc ccgttcctgg gtggggttgg gtgtgaacgg gtgcaatgcc
     3361 ggctctttcg gtgcgatgga aatcaagtcc cgctcctcgg ccagccaacc gatcgtatgg
     3421 agaaagaagt cgctattgcc ggggaagttg aaaaaggcgt tggacacgaa ggtcgagttc
     3481 ccgatcacca cgacggcggg tcgaggtttg ccctcttccg gagccttctt cggcgccagg
     3541 gcggcagcca tgggaagcgg tcctttcacg tcctcctttt cgctcaagct cacgacccgg
     3601 ccctgcatgt ttgtttcagc ccagctgttc ggcgaggtac gagccagcgg cacaaagtcc
     3661 cagtctttcc cgacctgctc atcgaacgtg acgtgtcgcg aaagcggcaa gagcacggcc
     3721 gaggtgaggt cctgcgtgat ctcatgctcg gtgaacgtcc gaaccagcag cgcggtgagg
     3781 tccccctgcg ccaatcgatc ctgtaaatcg accaacactc ccgggccaag accaagtccc
     3841 caatgggcca gcattggctc caggcctgtc tgagtgtcgg gatcggccaa gaccaggaga
     3901 tggccgccct tttcgacata agcctgaata cgttcctgtt cttccttcgt cacggaacgg
     3961 cgaggtccgg ccaacaccag caccgacgta ttctccggca cagcggattc ttttaacagc
     4021 gagagggtgc cgacgtcata accttgccgg gtcagggatt ctttggcgat agagaggccg
     4081 ttgcggtctt tgtcttccat gctcaactcg ctgtggcctt cgacgaagac gatgcgcttc
     4141 ttggcgtcct tggaaatgcg gatcagggcg ccggtcagct ccacttccga tggtgaagtc
     4201 acgcggatcg tctggccatt gctctcgaaa atcgcggtat cggttcggaa gatcccatag
     4261 ctttgggcga tcttcggctg tttttccgga tcgatgaatt cgacgctgag cttggtagag
     4321 gcttgccgat aactctccag tcgttccttg tatggttgat agccgggatc cttttccctc
     4381 gtgaagacgg tgatcttgac gtcgtgcggc aaggttcgta gcacccgatg ggtctgcgga
     4441 gcaagggtga agttctgatt ttccgacaga tcccaccgta ccgaatgccg cgacgcgaga
     4501 aaattcacga tgaccaaaat gccggaaaag agcacgatca tgagaaagct gttcagcccc
     4561 atcctggtcg accgtcggct ggagaatgcc ttgacggtct caaaatgcag aacgaagaac
     4621 agcaccaaac agaccagtgc cagcccctcc gcgatcgtag cgacccacag ccagtcaggt
     4681 cgaaggctgt acgtgatcat gccgccgatg ccgagcacga ctccgagaat gccgagagga
     4741 aaggatttca ggttcatttc cagcgtgtcg actccacgac gcgatgtgtg aggaacagca
     4801 tgagcgccag tccgcttccg aagtagatga ggtcgctcgt gtcgatcagt ccccgcacga
     4861 ggcggtcata atgttccatg tatgaaaagt atgagatgac gcggcctgca gtggtatctc
     4921 cgaacaggct tcccaatccg gcgatcagcc agatggtcag gagcatgccg aaactgacga
     4981 aggcggcgac gatttgattc tccgtcagcg ccgaggcaaa cagacccacg gcaagaaaca
     5041 gggctcccag cagcaccatt cctaaatagc cggtccagat gggattccaa tcgaagtcgc
     5101 tgaagagcat cagcaccgtc gggaccaatg tggttagtcc tacgaggccg agatagatca
     5161 agaacacact gacgaatttg ccgacgacga tttcattgat cctgattggc gaggtcatca
     5221 agaactcaaa tgtgcggagt ttgcgttctt ccgcgaacaa ccgcatggtc aagatgggca
     5281 gaataatcac gaggacgatc cgcatgctcg cgaagagatt ccggaaaacc agatcgttca
     5341 agttgatctg ggccatgcct ccttgcatct gcatcaattg gatggcttgc gcgccggcga
     5401 aaccgacgta aagataggag agcagcccga agatcagcag aaagaccgcg ccgacgacgt
     5461 agacaatggg agagacgaaa tagccgcgca gttccttggc gatgacggct tgcaccgggg
     5521 tcataaggct cctactcgtc cgtcctttcg gcggaggcac tgaccgtggc ttccaccggc
     5581 tcggaaaggt ggccctcgtg gcgcgtgagc tggaggaaga cgtcttccag cgtcatagat
     5641 atggtgcgga gctcaagcaa cccccactgg ctcataacgg ccacgcgtgc gatttcgtcc
     5701 cgtagatcgc ggcccagctc gcactcgatg agaaagcttc cccccgcctg tccaggcagg
     5761 acattgagaa tgccgtgaat cgagcgaagc ttcgtctcac agtccgacgg acatgtcttg
     5821 agggtaatcg agaccttctc cgattgacgc aatcgggccg acagctgttc aggggtatct
     5881 tcggcgacga tgcgaccttt attgatgatg accacccgtt ggcagactgc ggtggcctcc
     5941 gggagaatat gtgtgctgag aatcacggaa tgagagccgg cca
//
LOCUS       DATMTI010001027        10598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_632713, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001027 DATMTI010000000
VERSION     DATMTI010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10598
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632713"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..1672
                     /locus_tag="VN039_16265"
     CDS             476..1672
                     /locus_tag="VN039_16265"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV47552.1"
                     /translation="MIAQRSLTKSFLCVALFFGLQTLTGCQTPQTETYGGGQRKADAP
                     SAETAKHEMATTKSRADKDMAAVLTEFEDLKPKPIIALSAEEARAQPTPADAVAALLQ
                     ERKQSTAPMPIGKTKNQTISGPGGDIPIRIYTPQGNGPFPVIVYFHGGGWVMATIDTY
                     DSSARALAKSVDAIVVSVEYRKAPEHKFPAAHEDAYAAYQWVLRNIDSFDGDPSMVAV
                     AGESAGGNLAAAVCLMARARGELLPVYQALIYPIAGYDLNTSSYRENAHAKPLDKSMM
                     AWFFEKYLREPADGKHPWIDLVNARNLSGLPPATIITADIDPLRSEGKRYAERLLEAG
                     VPVSYQNFKGVTHEFFGMGAVVGDAKKAVRLVANGMNWSFDDPVFSRGDRLRSGPSVQ
                     IEPLQD"
     gene            1715..2233
                     /locus_tag="VN039_16270"
     CDS             1715..2233
                     /locus_tag="VN039_16270"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWV47553.1"
                     /translation="MSRFLSLSLMMVGAVGVASSVFAQTAKTDAQSFLETAAEGQQIG
                     ISLGQLAAQRAVNERVKDFGQQMVEDHKKGSQQVEQLAMKGGVQLSPGLSEEHKQKVN
                     NLSQLSGHAFDRAYIDYILEDHETTVDEFERRGKTIQNEDIKQWIASTIPTLQIHREK
                     AHQVKYSLQTNP"
     gene            2274..2519
                     /locus_tag="VN039_16275"
     CDS             2274..2519
                     /locus_tag="VN039_16275"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HWV47554.1"
                     /translation="MNEKTTDQSVLQRINKLVDQEHQMLVRETTNEEDRSRLQALQVE
                     LDQCWDLLRQRRALRTVGQDADNAQVRPPEIVEKYEQ"
     gene            2647..3033
                     /locus_tag="VN039_16280"
     CDS             2647..3033
                     /locus_tag="VN039_16280"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HWV47555.1"
                     /translation="MRTVIGPTLIIVISFSGIGCASSGPPVTPVQLADTEATIRSAES
                     AGAFERAPDLLQKARQALSVAQQASGKGDHRVARERLLEATTQAEAARARAQAEQKKS
                     EASMVHQQADELEAKVKLLQKQLQAP"
     gene            3095..4183
                     /locus_tag="VN039_16285"
     CDS             3095..4183
                     /locus_tag="VN039_16285"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV47556.1"
                     /translation="MQNRTWSTSGLLLGVLTIAACATPGPPQELVDARLAIQDAKSAN
                     ADKRATRTYDSAAANLDVANKSWEKDHDASAILHQARLAEAEARKAQYAAEAQAADEA
                     ILRENDRKTRHEIALRDAELMMLRKKGQETELERMKALAKEQEQAVLDAQEKLAAEQA
                     RAENEAARLRKEEEKLALAAEQARAEREAARLREEQDNLAALNAEQARAEQAERELAR
                     LRAEHDKSRAELTSTLSRLAKVRDEARGVIVTLPGNIYFNPNKADVKPAMQMQLTKIA
                     QALAAVPDQKLLIEGHTDSDGPNEYNLSLSESRAESVQKILLAGGIAAERMEIKGFGE
                     SKPIAGNSTPAGKAQNRRVEIVIVPTKQ"
     gene            4215..4664
                     /locus_tag="VN039_16290"
     CDS             4215..4664
                     /locus_tag="VN039_16290"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV47557.1"
                     /translation="MSVIEKSIEINVPVKTAYNQWTQFEEFPRFMEGVEQVRQIDDKH
                     LHFKASIGGKQKEWDAVITEQIPDQRIAWMSRGDSKNGGVVIFSKVSENKSKLNLRLE
                     YEPEGAVEKTGDTVGVVSGRVEGDLRRFKDFIESRGQETGAWRGKVA"
     gene            4784..6622
                     /gene="treZ"
                     /locus_tag="VN039_16295"
     CDS             4784..6622
                     /gene="treZ"
                     /locus_tag="VN039_16295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HWV47558.1"
                     /translation="MPEVLERDRPECGALFQEDGRVRWRLWAPKAERVDLVLIDGETR
                     VIHPMRREERGYFGLEKGQVRSGRRYAYRLNGGPERPDPASRWQPDGVNHPSAVLGLD
                     DFHWTDADWSGIAREDLVFYELHVGTFTPEGTFDAVIPRLPALRELGVTAIEIMPVAQ
                     FSGTRNWGYDGVHPYAPQQSYGGPQGLRRLVDACHRHGLAAFLDVVYNHLGPEGSYLS
                     EFGPYFTERYRTPWGAAVNYDDRGSDEVRAFVCNNACMWIRDYHFDGLRLDAIHAIYD
                     SGARHILPDIKDAAESASKGRGYAAHIVAESDLNDVRILLPPERGGYGLDAQWSDDFH
                     HAVHTCLSGERQGYYVDYGRPEQLAKALTDTFVLDGNYSRSRDRCHGGSNAGLPGDRF
                     VVCIQNHDQIGNRAAGDRLSALLSPPAQRLAASLLLFAPYLPLLFMGEEYGEPRPFQF
                     FCSFSDPTLIENVRNGRRREFEAFHKDGDDVPDPQAESTFAASGLSWSWESDPHRSGL
                     RRLYQDLLQARREWPALRDYVQRTARLLSTADAVLELNRGESGTDSQPAVRALFNLSA
                     QEQPIPGGNCDRFRWSSEAERYHGARRAHGPDRKLLPYECLVFNGI"
     gene            6902..8749
                     /gene="malQ"
                     /locus_tag="VN039_16300"
     CDS             6902..8749
                     /gene="malQ"
                     /locus_tag="VN039_16300"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWV47559.1"
                     /translation="MSRDAWDIDAVYQDAAGKEQRVSRESIEALRSAIGCPPSAPRSL
                     FDEPVKVVRHGEPLVIPGPAELRLEDGTAMEVRDRLPASVPVGYHELTPTSGSGTTKL
                     IVTPGECHLPSGLRIWGWSAQIYAMRSRSSWGIGDLADLRRLGEWAKSIGAGMVLINP
                     LNAALPVLPQEASPYSPSSRRYRNPLYLRIENVPGVERIETDLRQLAAGARALNDERK
                     IDRDRVFQLKLDALQKIWALFREDVDFERFQRNEGPSLDQFAVFNVLVEEHGTDWRQW
                     PSGYRRPDSGDMQRFAQEHAARVRFHKWLQWLLDRQLGRAAEPVTLMQDLPVGFSPNG
                     ADAWAWQDLVALDCSIGAPPDLFNTEGQNWGLPPFIPHRLRQAAYEPFIQTIRATMRH
                     AGGLRIDHVMGLFRLWWVPPGGIPKHGAYVRYQADDLLGIIALESRRAGAVVVGEDLG
                     TVEEGVREKMAECNMLSYRLVWFEDTAPADYPEKALAAITTHDLPMLAGIWTGKDFEL
                     QRRSGLDPDKAKAEKLRQKMVQISGVDLRADLETAAIQTFEQLAAAPSVIVMAAMEEA
                     CLASERPNMPGAEGTYPNWSLGLPHLLEEIEATPFPRRLAAILSRRNCA"
     gene            9163..>10598
                     /locus_tag="VN039_16305"
     CDS             9163..>10598
                     /locus_tag="VN039_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47560.1"
                     /translation="MNKSSARLSNVRITREAVKILLAVFLTGCVTAAAEDKKVPRPPH
                     DAVAQAQETRTVPIERPEQRKTDHDRSAIFDAQKARSSSPAFLDQPRDGKNSGFDFYR
                     DPLGAEKPLQEADEIMKKDIAAKPKVMETQRKFLERRYHLKPKLDSEAKMSRGKPLPV
                     GPTTRLPESMTWERLAGLSPEELKKQGLFPYPSLPHPLQSPGGMVFPQMQIDMFPRLE
                     RFDVDHDLPEAFLPEFPPAMFLSNRPELGDVSRGEVISINNYYRLFKDILTPVQLDGL
                     RMLLTPFPQEEFNPTDDRKSLHPSLGVTCFDCHVNGHTTGQFHLNPDTRPEERRFRLD
                     TTSLRGVFNQQIHSSKRSLRSIEDFTEFEQRTAYFNGDEIHAIKKGMNILDRIAVTHM
                     AQMQNMLDFPPAPKLDPMTGRLDPKKATENELRGEKVFFGKGQCAGCHTPPFYLDNKM
                     HDLHLERFLKDEPGDGPIKTFTLRGIKDS"
BASE COUNT         2435 a         2913 c         3218 g         2032 t
ORIGIN      
        1 cgtcgtaagc gacaaaaagg gtgacccgaa caaagtcttc gttgagactg cgggtggttc
       61 ccaacactca tgcaagtcat cgagcattac aaggggcatt tcaaactcgc cttaaccgag
      121 aatgagaaag ccgatctcgt ggagtacctg aagtcattgt aagtttagag cctctcgctt
      181 ttcgaacata ccgaccactc tcgtcgaaac tcagctgtgc caagcatgac cttcaccggg
      241 accgtaacgc gctctcggtt gttccgtaag aatttgcgcg gtaatttgac ctgcatgcat
      301 ctggggaaca tgccagtcgc cagctacttc tttaagcggt acaagaatag atggatgttg
      361 tcccataagg tagtgccgta acgctgccgt gtcggcgcga cgtggaggcg aggaacatcc
      421 gactcaaatc gggagaacgt gactacctca tcacactaaa aagaaaggaa gcgacatgat
      481 cgcgcaacga agtcttacga aatcattcct ctgtgtcgcc ctttttttcg gtctccaaac
      541 actgacgggt tgccaaacgc cacagacgga aacctacggc ggcggacagc gaaaagccga
      601 cgctccgtcc gccgaaacag cgaagcatga gatggcaact acgaagtcac gggcggataa
      661 ggacatggct gcggtgctga cggagtttga agacttaaaa cccaagccca ttattgcgct
      721 ctccgccgag gaagcacgag cgcaacccac acccgccgat gccgtggcgg cattgctgca
      781 agagcgaaag caaagtaccg ctcccatgcc gattggaaag accaagaatc aaacgatttc
      841 cggtcctggc ggcgatatcc cgatccgtat ctatactccc caaggtaacg gtccatttcc
      901 cgtaatcgtg tattttcacg ggggaggatg ggtgatggcc acgatcgata cgtacgattc
      961 gtcggcgcgg gctctcgcga aaagcgtgga tgccatcgtg gtgtccgtgg aataccgtaa
     1021 agctcctgag cataaatttc cggcggcgca tgaggatgcc tatgcagcct atcagtgggt
     1081 gttgcgcaat atcgacagct tcgatgggga tccttcaatg gtcgccgtag ccggtgagag
     1141 tgccggcggc aatttggcgg ccgctgtgtg cctaatggcg cgggcgcgcg gtgaactgct
     1201 gcccgtgtat caggcgctca tttacccgat agccggatac gatctcaaca cctcgtctta
     1261 tcgcgaaaat gcccacgcca agccgttgga caagtcgatg atggcttggt tctttgaaaa
     1321 atatttgaga gagccggcgg atggaaaaca cccgtggatc gatctggtga atgcccgcaa
     1381 cctaagcgga cttccacctg ccacgatcat tacggcggac atagatccct tgcgatcgga
     1441 gggaaaacgc tatgccgaac gtctcctcga agccggcgtt ccggtctcgt accaaaattt
     1501 caagggagtc acgcacgagt ttttcggaat gggcgccgtc gtcggcgacg caaaaaaagc
     1561 cgtgcgactg gtcgcgaatg gtatgaactg gtcgttcgac gatccggtct tttcccgcgg
     1621 cgaccgattg cgctctggtc caagtgtgca aatagaaccg ctgcaggact aggtcacatg
     1681 tacagtacgt gagcaatttc aagaggagaa cgctatgtcg aggttcttgt cattaagcct
     1741 gatgatggtt ggagctgtag gggtcgcttc gtctgtattc gcgcagactg ccaaaaccga
     1801 cgctcaatca tttcttgaga cggcggcgga aggacagcag atcgggatat cgttaggtca
     1861 acttgccgcc caacgtgcgg tgaacgaacg agtgaaggac ttcgggcagc aaatggtcga
     1921 ggaccacaag aaaggcagcc aacaagtcga gcaactggcc atgaagggcg gcgtgcaact
     1981 gtctcccggg ctcagcgagg agcataagca gaaagtgaac aacctctccc aactgtccgg
     2041 ccatgccttc gatcgtgcct acatcgatta tattctagaa gaccacgaaa ccacggtgga
     2101 tgagttcgag cggcgtggga agaccataca gaatgaagac atcaagcagt ggatcgcctc
     2161 gaccattccg actttacaga tccatcggga aaaggcgcat caagtgaaat actcgttgca
     2221 gacgaatcct taacgtatgg cggggaccgg atgagctgcc ggccgggtga tgaatgaacg
     2281 agaagacgac cgatcagtcg gtgctccaac gaattaataa acttgtcgac caggagcatc
     2341 agatgctggt tcgtgaaact acaaacgagg aggaccggag tcggttgcag gctctgcaag
     2401 tcgaactgga tcaatgttgg gatctccttc gtcaacgacg cgcgttacgg accgtcggac
     2461 aggatgccga caacgcgcag gtcaggccgc cggagatcgt cgagaagtat gagcaatgaa
     2521 accagcgatc gaagcctatc gaagggctgt ccgaatgttt ggcgcctgga attttatgaa
     2581 ttggggtagc gagtgcggct tagggtagag agattccacg agacgacgag acgaaaggag
     2641 ttccctatga ggactgttat tgggccaacc ctgataatcg taatctcatt cagtggaatc
     2701 ggctgcgcct cgagcggccc gccggtgacc cccgtgcaac tggcggacac cgaagcaact
     2761 attcggagcg ctgagtcggc aggagctttc gagcgcgctc cggatctcct gcagaaagcc
     2821 agacaggctc tcagtgtcgc ccagcaggct tcaggaaagg gcgatcacag ggtggcacgc
     2881 gagcgtcttc ttgaggcgac gacacaggcc gaagccgcac gagcccgggc tcaagctgaa
     2941 cagaagaaga gcgaggcctc tatggtgcat cagcaagcgg acgagcttga ggccaaagtc
     3001 aaactgctcc agaaacagct acaggctccg taacctgagc ttgccatgaa gtcccggaaa
     3061 taaggagctg agaggtgaaa ggaggggctc tactatgcaa aacagaacct ggagtacgag
     3121 tggccttctc ttgggtgtct tgactatcgc ggcctgcgcg actcccggcc cacctcaaga
     3181 gcttgtcgac gcacgacttg ccatccaaga tgccaaatcg gcgaacgcgg acaaacgcgc
     3241 gacgcgtacc tacgactcgg ctgccgcgaa cttagacgtt gccaacaagt cctgggaaaa
     3301 ggaccatgat gcgtcggcta ttcttcacca ggcacggctg gcggaagctg aggcgaggaa
     3361 agcgcagtac gccgcagaag ctcaggcggc ggatgaagcc atcctccgtg aaaatgaccg
     3421 gaagacccgt catgagattg ccctgcgcga cgctgagctt atgatgctga gaaaaaaagg
     3481 gcaggaaaca gaacttgaac ggatgaaggc cctcgccaaa gagcaagaac aggctgtgct
     3541 tgatgcccaa gaaaagctgg cggccgagca ggcccgcgcg gaaaatgagg ctgctcgact
     3601 tcgcaaagaa gaggaaaaac tggcgttggc agccgagcag gcccgcgcgg agcgtgaggc
     3661 cgcccgactt cgcgaagagc aggacaatct tgcggcgctg aatgctgagc aggcccgcgc
     3721 agagcaggca gaacgtgagc tcgctcggct tcgtgctgaa cacgacaaat cgcgcgcaga
     3781 gttaacctcc acgttgtcac gcctggcgaa ggtgcgcgat gaagcccgtg gagtcatcgt
     3841 cacgttgccc gggaacatct atttcaaccc caataaggcc gacgtaaagc cggcgatgca
     3901 aatgcagctt acgaaaatcg cgcaggctct ggccgcggtc ccggatcaga aattgctcat
     3961 cgagggccat accgattcgg atgggccgaa tgagtataat ctgtccctct ctgagtcgcg
     4021 tgctgagtcg gtccaaaaga ttcttcttgc cggagggatt gcggcagaac ggatggaaat
     4081 caaaggattt ggggaaagca aaccgatcgc gggcaacagc acgcccgccg gtaaagccca
     4141 aaatcgcagg gttgagatcg tgattgtgcc gacgaaacag taatagcgaa gggcacgaca
     4201 gaaaggaggc gactatgtcc gtgatagaaa aatcgatcga gatcaatgtt ccggtcaaga
     4261 ccgcctacaa tcagtggact caatttgagg agtttccccg cttcatggaa ggcgtggaac
     4321 aagttcggca gatcgacgac aaacatttgc actttaaggc cagcatcggg ggaaagcaaa
     4381 aagaatggga tgccgtcatc accgagcaga ttcccgatca acggatcgcc tggatgagtc
     4441 ggggagattc caagaacggt ggggtggtca tcttttccaa ggtttcagag aacaaatcaa
     4501 aactcaacct tcgtttggaa tatgagccgg agggcgccgt ggagaagacc ggcgacacgg
     4561 taggtgtggt ttccggacga gtggaaggag atctgaggcg tttcaaagat ttcatcgagt
     4621 cgcgcggtca agaaaccgga gcctggcgag ggaaggttgc ctgagaggac atgaatcgaa
     4681 tcgtacggcg tgcggtcagc cgccgacgga tttcaaagcg ggtcagcaac agcataggta
     4741 gcactgtcta cacggagcgc aaatttgacc atgatgggtc ggcatgcctg aagtgctgga
     4801 aagagatcgg cccgaatgcg gggcgttgtt tcaagaggac ggtagggtcc gatggcgcct
     4861 gtgggctccc aaggcggaac gtgtggactt ggtcttaatc gacggggaaa ctcgcgtcat
     4921 tcatccgatg agacgcgaag agcgtgggta ctttggtctt gagaaggggc aggttcgaag
     4981 cgggcgacgc tacgcctatc gtttgaacgg aggcccggag cggcccgatc cggcctctcg
     5041 ttggcaaccg gatggtgtca accatccttc ggccgttctt ggtcttgacg acttccattg
     5101 gaccgatgcg gactggagcg gcatcgcacg cgaagacctc gtgttttatg agttgcatgt
     5161 cggcacattc acgccagaag gcacgttcga tgcggtcatc ccccggctgc cggcgttgcg
     5221 cgagttgggc gtgacggcga tcgaaatcat gccggtggcg caattttccg gcacgcgcaa
     5281 ctggggatat gacggcgtgc atccctatgc gcctcaacag agctatggcg ggccgcaagg
     5341 tctgcggcgt ctcgtcgatg cctgtcaccg ccatggcctc gcggcgtttc ttgacgtggt
     5401 ctacaaccat ctcggaccgg aaggcagtta tctgagtgaa ttcggtccgt atttcaccga
     5461 gcgctaccgg accccctggg gagcggcggt caattatgac gacaggggtt cggacgaggt
     5521 ccgggcgttc gtgtgcaaca acgcgtgcat gtggatccgt gactatcact tcgacgggtt
     5581 gcggttggat gcgattcacg cgatttatga ctccggcgcc cgccacattt tgccggacat
     5641 caaggacgcg gctgagtccg catcgaaggg gcgaggctat gcggcgcata tcgtggcgga
     5701 aagcgatctc aatgacgtgc gtatcctgtt gccgccagag cgaggcggct atgggttgga
     5761 tgctcaatgg agcgacgatt tccatcatgc ggtgcacacc tgcctctccg gtgaacggca
     5821 aggatactat gtcgactatg ggcggcccga gcagttggca aaagcgctga ccgatacatt
     5881 cgtgttggac gggaactaca gccgttctcg tgatcggtgt cacggaggca gcaacgccgg
     5941 acttcccgga gaccgcttcg tggtctgtat tcaaaatcac gatcagatcg gcaatcgtgc
     6001 cgcaggcgat cgattgagcg cgctgttgtc tccgccggcg cagcggctgg cggcgagcct
     6061 gttgctgttt gcgccgtatt tgccgctttt gttcatggga gaagaatacg gcgagccgcg
     6121 gccgttccag tttttctgtt cgttcagcga tccgaccttg atcgaaaacg tccgcaacgg
     6181 acggcggcgg gagttcgagg cctttcataa ggacggcgat gatgtgccgg acccccaagc
     6241 cgagtcgacg ttcgcagcct ccgggctgag ctggtcctgg gaatcagacc cacaccggtc
     6301 gggactgcgt cgcctgtacc aagatctgct gcaggcgcgg cgcgaatggc cggcgctgcg
     6361 tgactacgtc cagcgcaccg cccgtctgct gtcgactgcc gatgccgtgt tggaactgaa
     6421 tcgcggagag tccggcaccg attcccaacc agccgttcgc gcgctcttca atttgtccgc
     6481 acaggagcag ccgattccgg gcgggaactg tgaccgcttt cgatggagtt ccgaggcgga
     6541 gcgctatcat ggcgcgcgac gcgcgcacgg acccgatcgc aagctgttgc cgtacgaatg
     6601 cctggtcttt aacgggatat gaaaaacttt tttgcaactg tggccaagag tccgcaggct
     6661 ggtcaaaaag gccgtccagc aaggccgcag cgaggtccgc gacgcgaaga ataatgagcg
     6721 tcacgtctgc ggacgggcgc gagtcggtga gcgcccagtg tcttaaaggc gaggcgtatt
     6781 ccttttcacc cacccgcccc gagctgctct tagcagctct aaccggtggt atgctgagcc
     6841 tctaagcgcc gcgagaacgc cgttggcgga ctatttcacc agcctgtgaa aggacaggcc
     6901 tatgagtcgt gacgcctggg acatcgatgc cgtctaccaa gacgcggcgg ggaaggagca
     6961 gcgggtctcc cgcgaatcca tcgaagcgct ccggtccgcg atcggctgtc cacccagtgc
     7021 gccacggtcc ttgttcgatg aaccggtcaa ggtagtgcgt cacggagaac cgttagtcat
     7081 ccccggtccg gcggagttgc ggctggaaga cggcacggct atggaggtgc gcgaccgact
     7141 cccggccagc gtgccggtcg gctaccacga gctcacgccg acgagcgggt ccggcaccac
     7201 gaaactcatt gtcacgccgg gagagtgcca tctgccctcg ggactacgaa tctggggatg
     7261 gagcgcgcag atctacgcca tgcgaagccg ttccagttgg ggcattgggg acttagccga
     7321 ccttcgccgc cttggggaat gggccaagtc cattggagcg gggatggttc ttatcaaccc
     7381 actgaatgcc gcgcttcctg ttcttccgca ggaagccagc ccctattcgc cttccagccg
     7441 ccgctatcgg aatccgctct atctccgtat cgagaatgtg ccaggggttg agcggatcga
     7501 aaccgatctt cggcagcttg cggcgggtgc tcgtgcattg aacgacgagc gcaagatcga
     7561 tcgcgatcgc gtgtttcaac tgaagctgga tgccttgcaa aaaatatggg cgcttttcag
     7621 agaagacgtg gacttcgaac gctttcagcg aaacgaagga ccgtcgctcg accaattcgc
     7681 cgtcttcaac gtgttagtgg aagagcatgg caccgactgg cggcaatggc cgagcggcta
     7741 tcgccggccc gactcggggg acatgcagcg gttcgctcag gaacatgccg ctcgcgtgcg
     7801 cttccacaaa tggctgcaat ggcttttgga ccgccaactg gggcgcgcgg cggaaccggt
     7861 gacattgatg caggacttgc ctgtgggctt ttctcccaat ggggccgatg cctgggcctg
     7921 gcaggatctc gtggcactcg attgctcgat cggagctccc ccggatctgt tcaacacaga
     7981 ggggcagaat tggggcttgc cgccgttcat tcctcaccga ctacgccagg cggcctatga
     8041 accattcatt cagacgattc gcgccacgat gcgccatgcc ggtgggctgc gtatcgacca
     8101 tgtcatgggc ctcttccggc tctggtgggt tcctccaggt gggataccga agcacggtgc
     8161 ctatgtgcgg taccaggccg atgatctgct gggaatcatc gcgctggaaa gccggcgagc
     8221 tggtgcagtg gtggtcggtg aagatctcgg cacagtcgaa gagggcgtgc gcgagaagat
     8281 ggccgagtgc aacatgctgt cctaccggct ggtctggttc gaggatacgg cgccggcgga
     8341 ctatccggaa aaggcgctag cggcgattac cacgcacgat ctccccatgc tggccggcat
     8401 ctggaccgga aaggatttcg aacttcagcg ccggagcggg ttggatccgg acaaggccaa
     8461 ggccgaaaag ttgcggcaga agatggtgca gatcagtgga gtggacctgc gcgccgattt
     8521 agaaacggca gccattcaga cgttcgagca attggccgcg gcgccctcgg tcatcgttat
     8581 ggcagcgatg gaggaggcct gccttgcctc ggagcgaccg aacatgccgg gagcggaagg
     8641 cacctatccc aattggtcgc tgggactacc ccacttgctc gaagagatcg aagcaacgcc
     8701 attcccgcgc cgcctggccg cgatcttgag ccgccgcaat tgcgcgtaac cgaagccgca
     8761 atgggaacct gccatgtgcg ctcagatcgc tacgtgccgc gtacacctgg acagttgtat
     8821 gaagcgcacc gcgaattgat cgcggagttt cccgtgtatc gaacctacat caatggttca
     8881 gacattaaag gctgatgggg cgacacggtg ctcgatggcc agcaggtcgt tagcgcagtg
     8941 catgtgacga gcacctcctg aatggaggca tccacggagg gcaaggaagt gccggcacca
     9001 gaggtacgcc tagtcgagca ggtgccgaga agagcgcata atacatacaa cttacggcgt
     9061 atcaggtgag gtgtttcaca gatgcaaatt tggcggatgc cgaacaagcc ggtggaaaaa
     9121 gacacgtatt aaagctgaag aaatagaaca atggaggtgc ttatgaacaa gtcttctgct
     9181 cgattgtcca acgtgcgcat cacgcgcgag gccgtgaaaa ttctgcttgc cgtctttctc
     9241 accggttgcg tcacggctgc cgctgaagac aaaaaggtac cgcgcccccc gcatgatgcg
     9301 gtggcccaag cgcaagagac tcgcaccgtg ccgatcgagc gcccggaaca gcgcaagacg
     9361 gatcacgacc gctccgccat cttcgacgcg cagaaggcaa gatcttcttc ccctgctttc
     9421 ctggatcagc cgagagacgg caagaactca ggtttcgatt tctaccgcga cccattggga
     9481 gcggagaagc cacttcagga agcggacgag atcatgaaaa aggatatcgc cgcgaagccc
     9541 aaggtgatgg agacgcaacg aaagttcctg gagcgccgct atcatctcaa gccgaaactg
     9601 gattccgaag ccaagatgtc gcggggaaaa ccgctgccgg taggtccgac gacgcgcttg
     9661 ccggaaagca tgacatggga acggctcgcc gggcttagtc ctgaagagct caagaaacag
     9721 ggactctttc cctatccttc gctgccgcac ccgctgcaat cccctggcgg catggtcttt
     9781 ccccagatgc aaatcgatat gttcccacgg cttgagcggt tcgatgtcga tcatgacctt
     9841 cccgaggcct tcctgccgga gttccctccg gccatgtttt tgagcaatcg tcccgaactg
     9901 ggggacgtgt cccgcgggga ggtgatctcc atcaataact actaccgcct gttcaaggat
     9961 atccttaccc ccgtccagct cgatggactg aggatgctgt tgacaccgtt tccacaggag
    10021 gaattcaacc cgaccgatga ccgcaaaagt ctccatccga gcttgggcgt gacctgcttc
    10081 gactgccatg tgaacggcca tacgaccgga cagttccatt tgaaccccga caccagaccc
    10141 gaggagcggc gctttcgcct cgatacgacc agcttacgag gcgtattcaa tcaacagatc
    10201 cactcatcta aacgcagctt gcgctcgatc gaggatttta cggagtttga gcaacggaca
    10261 gcttacttca acggcgacga aatccatgcc atcaaaaaag ggatgaatat cttggaccgc
    10321 attgcggtga cccacatggc acaaatgcag aacatgctcg attttccgcc cgcccccaag
    10381 ctcgacccca tgaccggtcg gctggatccg aaaaaagcta cggagaatga gctacgcgga
    10441 gaaaaggtct tcttcggcaa ggggcaatgc gcgggatgcc atacgccacc gttctatctc
    10501 gacaacaaga tgcacgatct gcatctcgaa cgtttcctca aggatgagcc gggcgatggc
    10561 ccgatcaaga cgtttacgct gcggggcatc aaagactc
//
LOCUS       DATMTI010001028         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_209717, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001028 DATMTI010000000
VERSION     DATMTI010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209717"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VN039_16310"
     CDS             complement(<1..940)
                     /locus_tag="VN039_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWV47561.1"
                     /translation="MRTIQYHWRIHFLTALLVVGSISLSLGAEPSEYILANGMKVLLV
                     EVPKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTARYPKGSFSRIVRKNGGI
                     DNAFTGQDFTAYFENVAADRVGLALELEADRMQGLILDHNEFQTERDVVKEERRLRSE
                     DDPQGALVEALFAQAFLSHPYHWPVIGWFADLDAMSLEDLQRHYDTFYSPNNATLVVV
                     GDIKAESLLPTIKRLFEPIPRGPSPKQTLSPEPEQRGERRFLLKREAQVPFVMMGFRV
                     PNYSNEDSYALDILESILSHGKSSRLYQSLVYDQKNS"
     gene            complement(969..2054)
                     /gene="rlmN"
                     /locus_tag="VN039_16315"
     CDS             complement(969..2054)
                     /gene="rlmN"
                     /locus_tag="VN039_16315"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWV47562.1"
                     /translation="MTPPDTTTMLLSLTEPQMVKFVRAHRWPDYRAKQILRWIHQRRL
                     RTITDMSDLPMHDRLTLSHSASIGRSPHVTVLPSRDGTRKLLLTLNDGMSIEAVLIPD
                     EDRLTLCVSTQVGCMLDCGFCLTGRMGLKRNLKLHEIIDQILTAQDLLSSDEHMTNLV
                     FMGMGEPLANLDNLKAAVTALTNKLWGLGWSRRRVTVSTAGLASRLSDVAALGVNLAI
                     SLNATTEEQRRELMPAASEIASLRSLLAACRRYPLPSHRRLTFEYVLLAGVNDHASDA
                     RRLVQLLRGMRCKVNLIPFNEFPGSLFHRPPEEAILRFQSILRDADLDAFVRKSRGRD
                     VLGACGQLGELSNNHPLLTLTPIETRC"
     gene            2220..4508
                     /locus_tag="VN039_16320"
     CDS             2220..4508
                     /locus_tag="VN039_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWV47563.1"
                     /translation="MHRSIGGCVLAVAFGLVLTLGCFLSSDGHTAQVETSEQSTANGC
                     TTAEDCFAAAAWPKERLGHALTKDQVMALKLERLRKIMEGFPATLWAKRAGLLSGVIL
                     IDRNPASALPYLRAAQRDFPVLDDYIRFWIGEALLRLGDAKEAAGMFEGVSQAVPDSN
                     LLNQVALRAGEAWYQASSCPEAVSWFVKAVNVNDKDAQISQAWSRLASCYLRENQLTE
                     GREALKQLWTKFPQTKEAKEAETLLGGNIGGVQWTAAPDMHYERAQAFLGQSLHAEAI
                     EELKKFLARDAFSPHRIDAKLKLGVAQVRLKLYDQARDTFQALTAEQGSRADEATVWL
                     ARVYLRQGLGEKLLDLCRTLSKRTMTPEQKGQLNLFAGIWLEDEARFDEAIARYRQVS
                     KFGEPASQRVEAYWREGWVLYRTARYREAITVWQQIVDQKDNDFESQALYWIARSYGQ
                     VEDVKSKEAFALLCQRYPYTYYCQLARERTDLSVSGQRRGEGAVVAASSTQLVPEVTQ
                     TSVQDNQTSSRTQIELQPAYRRAVELRTLGLEHDAARELSALTDRYSRDPEVLAALSM
                     MLNEVGAYHHALRLVRSRFREKLERTGGTVADGLWNVAYPTGLIPTIKMSGANGVDPF
                     LVAAIIREESQYDWRAVSRVGAIGLMQVMPATANAVAQQHRLPSLSREDLFDQEINIR
                     IGTRYIEQLFTQFAGNVVQTIAAYNAGPIVVGTWAVTYRGLSEDEFVELIQYQETRQY
                     VKRVLRSYKEYLRLAGVQKTVS"
BASE COUNT         1018 a         1173 c         1372 g          972 t
ORIGIN      
        1 acgaattctt ctggtcatag accaagctct ggtagagccg ggagcttttc ccgtgagaca
       61 ggatcgattc gaggatgtcg agagcatacg agtcctcgtt cgaatagttg ggtacgcgaa
      121 accccatcat gacgaacggt acctgcgctt cgcgcttcag gagaaaacgg cgctcgccgc
      181 gttgttccgg ttccggcgac agggtttgct tgggtgaggg acctctgggg atcggctcaa
      241 acagacgttt gatcgtcgga agtagagatt cggccttgat atcgcccaca accactaagg
      301 tcgcattgtt gggagagtaa aacgtgtcgt agtggcgttg caagtcttcg agagacatcg
      361 catcgagatc ggcgaaccat ccgatgacgg gccaatggta gggatgactg agaaaggcct
      421 gggcaaacag cgcttcaacc agtgcaccct gcgggtcatc ctcggacctg aggcggcgtt
      481 cttctttcac gacgtcgcgc tcggtctgaa attcgttgtg atcgagaatc aggccctgca
      541 tccgatcggc ctccagttcg agagccaacc cgacacgatc tgccgccaca ttctcaaaat
      601 aggcggtaaa gtcttgtccg gtaaatgcgt tgtcgatccc gccgttcttt ctgacgattc
      661 gggaaaacga gcccttcgga taccttgccg tgcctttgaa catcatgtgc tcgagcatgt
      721 gagagagccc cgcccgtccc atgacctcgt ttcgcgaacc gactttgtac cacacctgca
      781 ccgtggccac tggagccttg ggaacctcga ccaacagcac tttcatgccg tttgcgagaa
      841 tgtactcgct cggctccgct ccgagcgaga gcgagatgga gccaacgacg agaagcgccg
      901 tcaagaagtg gatgcgccaa tgatactgaa tagtccgcat gattatgatg aaggtggaac
      961 tcgttatgct agcaacgagt ttcgataggt gtcaaggtaa ggagcgggtg gttgttcgag
     1021 agttctccaa gctgtccgca ggcgccgaga acatcccgtc ctcggctctt tcgaacaaaa
     1081 gcatcgaggt cggcgtcgcg gaggatggat tgaaagcgaa gaatcgcctc ttcgggcggg
     1141 cgatgaaaaa ggctgccggg gaattcattg aacggaatca aattgacctt gcatcgcatg
     1201 cctctcagca attgcaccag tcttctggca tcagacgcat gatcgtttac accggcaagc
     1261 agtacatatt cgaaggtcag tcgtcggtga gaggggagcg gatagcggcg acaggcagcc
     1321 aggagcgatc tcagtgaagc gatttcgctg gcggcaggca tcaattctcg ccgctgctct
     1381 tcggtagtgg cattgagcga gatggcgagg ttcacaccaa gggccgcgac gtccgagaga
     1441 cgggatgcca gccccgccgt cgataccgtc acgcgtctac gcgaccaccc aagaccccac
     1501 aacttgttgg tcaaagcggt cacggccgcc ttaagattgt ccaaattggc caggggttct
     1561 cccattccca tgaacacgag attcgtcatg tgttcatcgg aactcagcag gtcttgcgcg
     1621 gtcaggattt gatcgatgat ttcgtgaagc ttgaggttgc gcttaagccc cattcgtccg
     1681 gtcagacaga aaccacaatc caacatacaa ccgacctgag ttgacacgca gagcgtcagc
     1741 cgatcctcat ccgggatcag cacggcttca atcgacatgc cgtcgttgag ggttaacagc
     1801 aatttgcgcg ttccgtcccg ggacggcagg accgtgacat ggggtgagcg cccgatcgag
     1861 gccgagtgag acaacgtcag gcgatcgtgc atagggagat cgctcatgtc ggtgatcgtt
     1921 cgaagccgtc gttgatggat ccagcgcaag atttgctttg cacgatagtc cggccagcga
     1981 tgcgcgcgga caaacttcac catctgtggt tcggtgagac tcaagagcat tgttgtggta
     2041 tcgggagggg tcattggcga gtcggcaata tcatctcaaa gctacaacct accatagcgg
     2101 cggtcatgcc gcatacggcc atgccaatca tcggggtcct gaaacttctt ccattaccga
     2161 cgactccgta tataataaac gcggacgagt gttggtagga cgtcatgaag agaaacatta
     2221 tgcatcgttc catcggtggc tgtgtgctgg cggttgcgtt cggcctggtg ctcacgctcg
     2281 gatgttttct ttccagtgat ggacataccg cgcaagttga gacgtccgaa caatcgacgg
     2341 ccaatggctg tacgaccgct gaggattgtt ttgcggcggc ggcttggccg aaggaacgcc
     2401 tggggcatgc gttgacaaaa gatcaagtga tggcgctcaa gctggaacgc cttcgaaaga
     2461 tcatggaggg atttcccgcc acgctgtggg ccaagcgtgc cgggttgctg tccggggtga
     2521 tcttgatcga tcggaatccc gcgagcgctc tgccctacct tcgggcggcc cagcgggact
     2581 ttcccgtgct cgacgattat attcgattct ggatcggaga ggctttgctg cgcttgggag
     2641 acgctaagga ggcggccggc atgtttgagg gggtgtcgca agcggtcccc gattccaatc
     2701 ttctcaatca ggtggcactc cgtgccggag aagcgtggta ccaggcgtcc agctgtcccg
     2761 aggcggtgtc atggttcgtc aaggccgtca atgtcaatga taaggacgcg cagatctctc
     2821 aggcctggtc gcggttggcg tcttgttatc tccgagagaa tcaattgacg gaaggacgag
     2881 aagcgctgaa gcagctttgg acgaagtttc cgcagacaaa agaggccaag gaagccgaga
     2941 ctctgctggg aggcaatatt gggggagtac agtggacagc ggcacccgac atgcattatg
     3001 agcgtgccca agctttccta ggacagtcgc tccatgcgga ggcaatcgag gagttgaaaa
     3061 agttcttggc gcgagacgcg ttttctcctc accgcatcga tgccaaactc aaattgggag
     3121 tcgcccaagt tcggttgaag ctgtacgatc aggcacgcga cacctttcag gcgctgactg
     3181 ctgagcaagg atctcgggcg gatgaagcga ccgtatggct cgcgagggtc tatctcaggc
     3241 agggactcgg ggagaagtta ttggatctct gccgaacact ctcgaaacga acgatgactc
     3301 cggaacagaa aggacagctc aatttatttg ccgggatttg gttggaagat gaggcgcggt
     3361 tcgatgaagc gatcgcaagg tatcgacagg tgtccaagtt cggagagccg gcttctcaac
     3421 gagtggaagc atactggcgg gaaggatggg tgctttaccg aacggctcgt tatcgtgagg
     3481 cgatcaccgt atggcagcag atcgtcgatc agaaggacaa cgactttgaa tcccaggcgc
     3541 tctactggat tgcccgttcc tacggtcaag tagaagatgt gaagtcaaaa gaggcgtttg
     3601 ccctgctctg ccagcgctat ccgtacacct actactgcca gttggcgcgt gaacgcacag
     3661 acctgtcggt ttccgggcag aggagaggag aaggggccgt cgtcgctgct tcgtccactc
     3721 aactcgtccc tgaggtaacc cagacctcgg ttcaggacaa tcagacaagc agtcgcacac
     3781 agattgagct gcagccggcg taccgacggg cagtggaact caggacactc ggtctcgagc
     3841 acgatgccgc cagagagctc agtgcattga cggatcggta tagtcgtgac cccgaggtgt
     3901 tggcggcgtt atcgatgatg ttgaacgagg tgggtgctta tcaccatgcc ctgcgcctgg
     3961 tacggtctcg attccgtgag aagctagagc gaaccggcgg aacggtcgca gacggactgt
     4021 ggaacgtggc ctatccaacc ggattgattc ccacgattaa aatgtcggga gccaacgggg
     4081 ttgatccgtt tctcgttgcg gcgatcattc gggaagaaag tcaatatgat tggagagccg
     4141 tctcgcgtgt cggggcgatc ggattgatgc aggtcatgcc tgccaccgct aacgccgtgg
     4201 ctcaacagca tcgtctccca agcctatccc gagaagattt gttcgatcaa gagatcaaca
     4261 tccgaatcgg gactcggtat attgagcaac tgtttacgca gtttgccggc aatgtggtgc
     4321 agacaatcgc cgcgtacaat gccggtccaa tcgttgtggg aacctgggcg gtgacctatc
     4381 gggggctgag cgaagatgaa ttcgtcgagt tgattcagta tcaggagacc aggcagtacg
     4441 tcaaacgcgt cctgcgtagc tacaaggaat acctgcggct ggcgggggtt caaaagaccg
     4501 tttcttgaca agattttgtg aagtttttat agtgc
//
LOCUS       DATMTI010001029         3617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_122932, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001029 DATMTI010000000
VERSION     DATMTI010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122932"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /gene="rplI"
                     /locus_tag="VN039_16325"
     CDS             <1..433
                     /gene="rplI"
                     /locus_tag="VN039_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWV47564.1"
                     /translation="GHLGDLINVADGFARNYLLPRRKAVEADGRSIKAFEHVKRVAAE
                     KAKKEKLEIESYAKKVSAIALTIEAQVGKDDKMFGSVTAKDIAEGLAAQGVTVDRRKI
                     QLAQPIKELGTAVVPIKMPRDVTATVTVRVVKKHEPEEPST"
     gene            444..1718
                     /gene="glyA"
                     /locus_tag="VN039_16330"
     CDS             444..1718
                     /gene="glyA"
                     /locus_tag="VN039_16330"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWV47565.1"
                     /translation="MEKMVGDAIGSWEALRATDPEVYAAIEAEEVRQREKLLLIASEN
                     FASPAVLAAQGSLLTNKYAEGYPGRRYYGGCQHADAVEDLAIQRCKEIFGAEHVNVQP
                     HSGSQANMAAYLSVLKAGDTILGMDLAQGGHLTHGSKVNFSGILFRVFSYGVDRRTET
                     IDYDAVQKVAEECRPRMIVVGASAYARVLDFPRFQQIAKSVGAYLLVDIAHIAGLIAA
                     GLHPNPVPYADFVTTTTHKTLRGPRGGVTMCKAEYAKGVDKLVFPGLQGGPLMHVIAA
                     KAVAFKEALSPGFKRYQQQVLTNAKALAQGFIDRGYKIVSGGTDTHLMLLNLTNKGIT
                     GKEADAALDTAGIIVNKNAVPYDEKPPAVASGIRLGSPIVSTRGMKEPEMKRIVELVD
                     RVLQHRQEPTVLEEVRSQAKALCAGFPIIHPY"
     gene            1751..2215
                     /gene="nrdR"
                     /locus_tag="VN039_16335"
     CDS             1751..2215
                     /gene="nrdR"
                     /locus_tag="VN039_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWV47566.1"
                     /translation="MKCPFCDELEDKVVDSRMAKEGEVIRRRRECLGCKRRYTTYERV
                     EEILPVVVKKDGRRESFDRNKILVGLKKACEKRPISIGTIEAVTDRIEKRIQEMGETE
                     IESRVVGEEVMKELHQLDQVAYVRFASVYREFKDIDQFMDELKTLAQQRRER"
     gene            2190..3431
                     /locus_tag="VN039_16340"
     CDS             2190..3431
                     /locus_tag="VN039_16340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWV47567.1"
                     /translation="MLNNAANVKTQLPAPVTLFPGSFLPSDRRSPVVNSDELYGGGAE
                     TMNKPLSQQRARRNQPTSGATSVAIIGAGRGGTALMEIFATDPLVQIVGVAERDPEAP
                     GLGLAKQLGIPITRDYRQLLAMEPVDLIIDVSGDAEVRQFLQDFHRMGVTIIGGASAK
                     FMWELIEARIRATAEIEKTLNKYQSLYRLYVKETGAAVTEERTRIACEIHDGFVQSLA
                     GVNFKLDLCQQLIRKNPRAGLATLKESKAQLKLAIQEARQVIFNLRPLHYDKMELIPA
                     LTNYFKSYQTQTHIATKFSVTGDEQILFPRTKIFLFRIIQEALSNVEKHAKADRVSIK
                     LDIDMDLLRVTISDNGIGFDLETVLRDPEKWDHFGIKGILERARLVGGEGHVQSKPGK
                     GTKIIVEVPLGHKEERGNGEN"
     gene            3418..>3617
                     /locus_tag="VN039_16345"
     CDS             3418..>3617
                     /locus_tag="VN039_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV47568.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKEDIHVLGEAQDGMEAVEK
                     ARSLLPDVILMDVSMPRMGGVEA"
BASE COUNT          861 a          949 c         1095 g          712 t
ORIGIN      
        1 ggggcacctc ggcgatctca tcaacgtcgc cgatgggttt gcgaggaact atctgttgcc
       61 gcgtcgcaag gccgtcgaag ccgacggtcg gagcatcaag gccttcgagc atgtcaaacg
      121 agtggcggcc gagaaggcta agaaggaaaa gcttgaaatt gagtcctatg ccaagaaggt
      181 gtcggcgatc gccctgacga tcgaggcgca ggtcgggaaa gacgataaga tgttcggttc
      241 cgtcaccgcc aaagacatcg cggaaggatt ggccgcgcaa ggtgtgacgg tggaccggcg
      301 aaagatccaa ttggcgcaac cgatcaagga actcggtaca gcggttgtcc cgatcaagat
      361 gccgagggac gtgacggcga ccgtgaccgt ccgtgtagtg aagaagcacg agcctgaaga
      421 gccttcaaca taggaatgtg aatatggaga aaatggtcgg cgacgcaatc ggttcatggg
      481 aggccctgag ggctacagat cccgaagttt acgccgcgat tgaggcggaa gaagtccgtc
      541 agcgggagaa gttactattg attgcgtcgg aaaattttgc cagccctgcg gtattggccg
      601 cacaaggttc cttgctcacc aataagtatg cggaagggta tcccggcaga cgatactacg
      661 gcggatgtca gcatgccgat gccgtcgaag atttggcgat tcagcggtgc aaagaaatct
      721 tcggtgccga acatgtgaac gtgcagccgc attccggttc acaagccaac atggcggcct
      781 atctgtcggt tctgaaggcg ggcgacacca ttctcggaat ggaccttgcc caaggcggtc
      841 acctcacgca tggcagcaag gtcaatttct ccggtatcct cttccgtgtc ttctcctatg
      901 gcgtcgatcg ccggaccgaa acgatcgact acgatgccgt ccagaaggtc gcggaggaat
      961 gccgtccgcg gatgatcgtg gtcggggcca gtgcctatgc ccgtgtgctg gattttcccc
     1021 gtttccaaca gatcgccaaa tcggtcggag cctatctctt agtcgacatc gcacatattg
     1081 caggcctcat cgctgcgggg cttcatccga atcccgttcc ctatgccgac ttcgtcacga
     1141 cgacgactca taagacgctc aggggcccgc gcgggggcgt cacgatgtgc aaagccgaat
     1201 atgcgaaggg agtcgacaaa ctggtatttc cgggactaca gggcggacct ttgatgcatg
     1261 tgatcgcggc caaggcggtc gccttcaaag aggcgttgtc gccgggattt aagcgctatc
     1321 agcagcaagt tctgaccaat gcgaaggcct tggcacaagg gttcatcgat cgcggctata
     1381 agatcgtctc cggaggaacc gatacgcatc tgatgctcct gaacctcacg aacaagggga
     1441 tcaccgggaa agaggcggat gccgcgctgg acacggccgg cattatcgtc aataagaacg
     1501 ccgtgccata cgatgaaaag ccgccggcgg tggccagcgg gatccggctg ggatcgccca
     1561 tcgtttccac gcgcgggatg aaagaaccgg aaatgaaacg gatcgtcgaa ttagtcgatc
     1621 gtgtcctcca gcaccgacaa gaaccgacgg tgttggaaga agtgcggagc caggcgaaag
     1681 cattgtgcgc cggatttccc atcattcatc cctactagtc agccgatagc gagagagcag
     1741 gtcgccgccg gtgaaatgtc ccttctgcga tgaacttgaa gacaaggtcg tcgattctcg
     1801 tatggccaag gaaggcgagg tcattcgtcg ccgccgagag tgccttggtt gtaaacgccg
     1861 ctataccacg tacgagcgag tcgaggaaat tcttcccgtt gtcgtgaaga aggacggtcg
     1921 ccgcgagtcg tttgaccgca ataagattct cgtcggcctg aaaaaagcct gtgaaaagcg
     1981 gccgatcagt atcggcacga tcgaagccgt caccgaccgg atcgaaaaac ggatccagga
     2041 gatgggcgag acggagattg aaagtcgggt cgtcggtgaa gaagtcatga aggagctgca
     2101 tcagctggac caggtcgcct atgtccggtt tgcatccgtc tatcgggaat ttaaggacat
     2161 cgaccaattt atggacgagt tgaagacgct tgctcaacaa cgccgcgaac gttaagacgc
     2221 aactcccggc tcccgtcact cttttccccg gctcgttcct cccatccgat cggcgttcgc
     2281 ccgtcgtcaa ctccgacgaa ttgtatggag gaggtgcaga gacgatgaat aagccgctct
     2341 cccagcagcg cgctcgacgg aaccagccga cgtccggagc gacgagcgtc gcgattatcg
     2401 gggcaggtcg cgggggcact gcgctcatgg agatttttgc gactgatccg ctggtgcaaa
     2461 tcgtcggtgt tgccgaacgt gatccggaag cgccggggtt gggattggcc aaacagctcg
     2521 gcatcccgat tacacgcgac tatcgacagc tgttggcgat ggagcctgtc gacctgatca
     2581 tcgatgtatc gggtgatgca gaggtccggc aatttctcca ggattttcat cgaatgggtg
     2641 tcaccatcat cggcggtgcc agcgccaaat tcatgtggga gctgatcgaa gcccgcattc
     2701 gcgcgacggc agagatcgag aaaacgctga ataagtatca atccctgtat agattgtacg
     2761 tgaaggagac cggagctgca gtgacggaag agcggaccag gatcgcgtgc gaaatccacg
     2821 acggattcgt gcagagcttg gcgggtgtga acttcaagct ggatctttgc cagcaactta
     2881 ttcgaaagaa cccgcgagcc ggcttagcga ccctcaagga gagcaaggcc caactgaagc
     2941 tggccatcca ggaggcccgc caagtgatct tcaatctgcg cccactgcac tacgacaaga
     3001 tggaattgat tcccgccctt acgaattatt tcaagtcgta ccagacccaa actcacattg
     3061 ccacgaagtt ttcggtgacg ggggatgaac agattctctt cccacggacg aagatctttc
     3121 tttttcgaat cattcaggaa gccttgagca acgttgaaaa gcatgccaaa gccgaccgag
     3181 tctccatcaa gctggatatc gatatggatc tgttgcgcgt gacgatttcc gacaatggaa
     3241 tcggcttcga cctggaaacc gttctacgtg accctgaaaa atgggatcat ttcggcatca
     3301 agggtatctt ggaacgagcc cgcttggtgg gcggcgaagg gcatgtccaa tccaaaccgg
     3361 gaaaaggcac gaaaatcatc gtcgaggtgc cgctgggtca caaggaggag cgagggaatg
     3421 gagaaaatta aggttctgat cgccgacgac catcgggtgg ttcgagaggg cttggccgcc
     3481 attctcaaga ccaaggaaga tattcatgtc ctcggcgaag cgcaagacgg gatggaagcg
     3541 gtggagaaag cccgctcgtt gctccccgat gtgattctca tggatgtcag catgccgagg
     3601 atgggcggcg tcgaggc
//
LOCUS       DATMTI010001030         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_252684, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001030 DATMTI010000000
VERSION     DATMTI010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252684"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VN039_16350"
     CDS             <1..695
                     /locus_tag="VN039_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47569.1"
                     /translation="VGGLIWVLRHMSVGWYWGDGIERPEKFAEDLKAVIRDQHIDQHT
                     VVRGQDLLQSGQCRLSILNPPEPSVVRESTQPSTGTVLNNLSIVSRLQCGTYSILFAA
                     DIETAGLRRLDGEGYQPVTVLKVPHHGARSSLDQNWIRQLRPQYAVVSVGAGNPYGHP
                     VDSVLQAYQDQHITVFRTDRDGAIWVTGRLSTSEFTVSRMRDLIVQPVDFPHCLWRCE
                     YQNWHRLSLQFS"
     gene            925..1101
                     /locus_tag="VN039_16355"
     CDS             925..1101
                     /locus_tag="VN039_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47570.1"
                     /translation="MSKLVRIVVQLPVELKEQLDALKQQGYTTSGFIRATLERELNKL
                     NPRPDPAVRKVVNK"
     gene            1243..1494
                     /locus_tag="VN039_16360"
     CDS             1243..1494
                     /locus_tag="VN039_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47571.1"
                     /translation="MSPDLTGPRSWKRMLRAWGTILFLCLLGAVAAIGVRVLIESAGG
                     AKHTRYESVDVPPQADSPRHEREREEHERQGRVVLEKEK"
BASE COUNT          347 a          389 c          435 g          356 t
ORIGIN      
        1 acgtcggcgg gctgatttgg gtgctccgac atatgtcagt gggatggtac tggggcgacg
       61 gaatagaacg ccctgagaaa ttcgctgaag atctcaaggc cgtcattcgt gatcaacata
      121 tcgatcaaca caccgtagtg cgtggccaag acctgttgca gtccggtcag tgccgactga
      181 gcattctcaa tccaccggaa ccatctgtag ttcgtgaatc gactcagccc tctaccggaa
      241 cagtactgaa caatctgtcc atcgtctcgc gactgcagtg tggaacctat tccatccttt
      301 tcgcagctga tattgagact gctggattga ggcgattaga cggggagggg taccaaccgg
      361 tgaccgtcct aaaagtgcca catcacggag cgcgtagctc actggatcag aactggattc
      421 ggcaactccg cccacagtat gccgtcgtct cggtgggagc gggtaatccc tatggacatc
      481 ccgttgattc ggtactgcaa gcttatcaag atcagcacat tacggtcttt cgcacggatc
      541 gtgacggggc gatctgggtt acaggacggc tctcaacatc cgagtttaca gtgagccgca
      601 tgcgggatct tattgtgcaa cctgttgact ttccgcattg tctttggcgt tgtgagtacc
      661 agaattggca ccggctctcc ctccaattct cataacgaca agcattcctc ctttccggct
      721 gtccaagctc catcctcccg caaccggtat ccttctgtac aagaccggta atgcatgaca
      781 gtttttggct cttcccggca gaaatgctga gagagcacag cataagtgtc agtattttcg
      841 caccgttaac gagatgctag caaggcatag catgtgcaaa agtcccatgt caggttagta
      901 ctttgtctca agggagctgc gatcatgtcc aagcttgttc gcattgttgt tcaacttcct
      961 gtcgagttga aggagcagct tgatgccctc aagcaacagg gatacacgac gagtggtttc
     1021 attcgcgcca ccttggagcg ggagctgaat aagctcaatc cacggccgga tcccgcggtg
     1081 agaaaggtcg tgaataagta agggaccgca gctcacagga tgtaccgatc tcgccggacc
     1141 gttagggccg ctcctcaatc acagcgacga actcgtcagg tgagtcttgg atggctgaag
     1201 actatgtcct agacaatgct ctcaacgatc aggaaatcgg aaatgagtcc agatctcaca
     1261 ggccctcggt cttggaagcg gatgctgcgc gcgtggggga cgattctctt tctttgtctc
     1321 ttgggagcgg tggcggctat tggcgtgcga gtactgattg agtcagccgg aggcgccaag
     1381 catacgcggt atgagtcggt ggacgttcca ccgcaggccg attcgccgcg tcatgaacgg
     1441 gaacgagaag aacacgaacg acagggtcgg gttgtgttgg agaaggagaa gtagaggatg
     1501 tgttcgcggc aggagtggtg attgctt
//
LOCUS       DATMTI010001031         1595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_506388, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001031 DATMTI010000000
VERSION     DATMTI010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506388"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="lipA"
                     /locus_tag="VN039_16365"
     CDS             complement(<1..904)
                     /gene="lipA"
                     /locus_tag="VN039_16365"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWV47572.1"
                     /translation="MSFVPLDHLRSSSTINHQLSSVSRPRLPSWFKVQAKTGPDYLAI
                     KQTMDRLKLHTICEEARCPNRWECWNARTATFLILGDICTRRCHYCSVQTGKPFPVDE
                     EEPGRVAEAVRALGLRHAVITSVNRDELPDGGAWVFAETIRQTRRLNPTCTIEVLIPD
                     FEGDEKALATVCAEKPDILNHNIETVRRLFPSIRPQGKYQRSIELLAKAKQQGMKTKS
                     GLILGMGETLDEAREVMRGLRVVDCDIVTIGQYLQPTRDHLPVARFYDPSEFALLKEE
                     GLAMGFTHVESGPLVRSSYHAEQQV"
     gene            complement(901..1275)
                     /locus_tag="VN039_16370"
     CDS             complement(901..1275)
                     /locus_tag="VN039_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47573.1"
                     /translation="MNGGALDKHQLAGLDNKERGFSRPVEFERVGEGYRAILRYETVR
                     VTTERHPSQDDALAILIQTLHIQGYRQLKTQLSFRNGVYLGSQELWVEYPDPPQTEPE
                     PSGFFDKIKNWFRPRSVDGEQS"
     gene            complement(1279..>1595)
                     /locus_tag="VN039_16375"
     CDS             complement(1279..>1595)
                     /locus_tag="VN039_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47574.1"
                     /translation="LYVFLAQYLDRCFDEAARKSYPDSELQRHLEETRRQLMEILSVN
                     PVVKRKLADECDRILTLGASCLKSGAVDSKTREMIQAERTILQNKTIALSDLVAVYRA
                     LA"
BASE COUNT          351 a          440 c          421 g          383 t
ORIGIN      
        1 ctacctgctg ctccgcgtga taggagctgc ggacgagcgg acctgattct acgtgagtga
       61 accccatagc gagaccttct tctttcagca aggcgaactc ggagggatca tagaaccggg
      121 cgacgggcag atggtctctc gtcggctgaa gatattggcc gatggtgacg atatcgcaat
      181 cgacgactct gagaccccgc atcacctcgc gcgcttcgtc gagtgtctcc cccatgccca
      241 agatcaagcc ggacttcgtc ttcatcccct gctgcttggc ctttgccagt agctcaatcg
      301 accgttgata ttttccttga ggtctaatcg atgggaagag tcgccggaca gtttcgatat
      361 tgtgatttag aatatccggc ttctcggcac agaccgttgc gagcgctttc tcgtcaccct
      421 caaagtccgg tatcaggact tcgatcgtgc aagtcggatt cagccgtctc gtctgcctga
      481 tcgtttccgc aaagacccag gctccgccat ccggcaattc atcacgattc actgaggtga
      541 tcaccgcatg gcgcagaccg agggctcgca ccgcttccgc gacacgacct ggctcctcct
      601 catccactgg aaacggcttc cctgtctgca cagaacaata atgacagcgt ctggtgcaga
      661 tatcgccgag aatcaggaac gttgccgtac gggcattcca acattcccat cgattcggac
      721 agcgagcctc ttcgcagatg gtgtggagtt tgagtcgatc catggtttgc ttgatagcga
      781 gataatccgg accggtcttt gcctggacct taaaccagga aggaaggcgg ggtcggctga
      841 cagacgagag ttgatggtta atggttgatg aggaacggag atggtccaat ggaacaaaac
      901 tcatgactgc tctccatcca cagagcgcgg tcggaaccag ttctttattt tatcaaagaa
      961 accggatggt tcaggttcgg tctgtggtgg atccggatac tccacccaga gttcctgtga
     1021 tcccagatag accccattgc gaaaactcaa ttgagtcttg agctgtctgt atccttgaat
     1081 gtgcaaggtc tggatcaaga tggcaagcgc gtcgtcctgg gacggatgtc tctccgtcgt
     1141 gacgcggact gtctcatacc gcaagatggc ccgatatcct tccccaacac gttcaaactc
     1201 gaccggccga ctaaatcctc gctccttatt gtccaatccg gcgagttgat gcttatcgag
     1261 tgctcctccg ttcatcgttc atgctaaggc tcgatagacc gcaaccaagt cgctcagcgc
     1321 aatggttttg ttctggagta tggtccgttc agcttgaatc atctcgcgag ttttggaatc
     1381 gacggcaccg gattttaaac aagaggcacc gagcgtcaga attcggtcac actcatcggc
     1441 aagcttcctc tttacgaccg gattgacgga cagaatctcc atgagttgcc gacgtgtttc
     1501 ttcgaggtgc cgttggagtt cggagtccgg gtagctcttc cgagccgctt catcgaaaca
     1561 ccgatcgaga tactgggcta gaaagacata gagac
//
LOCUS       DATMTI010001032         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_548998, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001032 DATMTI010000000
VERSION     DATMTI010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548998"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VN039_16380"
     CDS             complement(<1..1427)
                     /locus_tag="VN039_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HWV47575.1"
                     /translation="MATAIEPVERNQLIKHPDVVISVGVVAILMVMLLPLPRFVLDLL
                     LSFDITLSVVILLVGLQVRRPIEFSVFPSVLLMITLFRLSLNIASTRLILLHGNEGAG
                     AAGEVIRAFGNFIVGGNYTVGLVVFSILVIINFVVVTKGAGRVAEVAARFTLDAMPGK
                     QMSIDADLNAGLINEADARRRRREIAEESDFYGAMDGASKFVRGDAIAAVIIILVNIV
                     GGLAIGILQQGMSPALAAQTYTILTVGEGLVAQIPALIVSTAAGIVVTRAASETDLGS
                     EMTRQLLMSSKPVGIAAGILLALGLVPGLPHVAFLALGSAIAWIAYQLHQQEQVQTAP
                     ILPPATPKAEEGLTRVIPLDLMEVQVGYGLIGLVEGTQGTALLDRIKALRRQFAESMG
                     FVVPPIHIRDNLQLRPNEYAIILKGVEVAKADVLPGHLLAIDPGTGQRGLVQGIATKE
                     PAFGLPALWVPEEAREQAQMAGYTVV"
     gene            complement(1440..2528)
                     /gene="flhB"
                     /locus_tag="VN039_16385"
     CDS             complement(1440..2528)
                     /gene="flhB"
                     /locus_tag="VN039_16385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HWV47576.1"
                     /translation="MAEDRSNKTEPATPKRKEEARKKGQIAVSRDLSIAVILLSGIGL
                     LGAMLPIGVQKMTEMTRQGLALSFPLDFHEGMSIEQVYSVVIHAGLTVVTLSLPVVIG
                     ILVMGSAASLLQTGLLWRANAVQPDVERISPIKGLSRLFSLRSVMELVKGLLKIAIVT
                     GIGLWVARYDVLQIPGLIGFDLGTALQVTGGLSLKVSLAVAGAIAVLAGLDYFYQRYE
                     WERSLRMSKDEIKEEHKATEGDPLIKSRVRTIQREMTKKRMMAAVKTADVVITNPTHL
                     AVALKYDTATMGAPVVVAKGAGLVAERIRELARHHGVPVVEHKFVARTLFKLVDIGKE
                     IPNELYRAVAEILAFVYRARGVTSLRNS"
     gene            complement(2521..>2872)
                     /locus_tag="VN039_16390"
     CDS             complement(2521..>2872)
                     /locus_tag="VN039_16390"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HWV47577.1"
                     /translation="LVATILSSYESIPAFGASLPGGMGEDILRLFQHMFLVGLKLAAP
                     VLVVILLINILLALLGRAVSQINVFVLSFPVTIAGGLAVMGLSIPFVVEFLVREIERL
                     QFTIDGIMKALGRG"
BASE COUNT          688 a          893 c          732 g          559 t
ORIGIN      
        1 accacggtat acccggccat ttgagcctgt tccctggcct cttcagggac ccagagtgct
       61 ggcaatccga aggcaggctc cttggtcgcg atcccttgca ccaaaccccg ctggccggta
      121 ccaggatcga tcgccaacag atgccctggt aagacatcag ccttggccac ttccacgccc
      181 ttcaaaatga tggcatattc gttggggcgc aactgaagat tgtcccgtat atgaatggga
      241 ggcacgacaa agcccatcga ttccgcaaat tgtcgccgga gcgctttaat ccgatcgagc
      301 agtgcggtgc cttgcgttcc ctcaacgagt cctatcaagc cgtaccccac ttgaacttcc
      361 ataaggtcga gcggaataac tcgggtcaag ccttcctcgg ccttcggagt agcaggggga
      421 agaatcggag cggtttggac ctgttcctgc tgatggagtt gataggcgat ccaggcaatg
      481 gcgctcccta acgccaagaa cgccacatgc ggaagccccg gcaccaatcc aagcgcgaga
      541 agaatacccg cagcaatgcc caccggcttg gaggacatca acagctgtcg agtcatctcg
      601 ctccccaaat ccgtttccga agcagcgcga gtcacgacga taccggcggc agtcgaaacg
      661 atcagggcgg gaatctgcgc caccagccct tcaccgaccg tcagtatcgt ataggtttga
      721 gccgcaagtg ccggactcat cccctgttgg aggataccga ttgccaatcc accgacgata
      781 ttgacgagta tgatgatgac ggctgcaatg gcatctcctc gaacaaattt actggcaccg
      841 tccatcgcac cgtaaaagtc cgactcttcc gcaatttcac gtctccggcg gcgcgcgtcc
      901 gcctcattga tcagaccggc attcagatcc gcatcaatgc tcatctgctt tccgggcatg
      961 gcatccaatg tgaatcgggc ggcgacttcc gccacacgtc cggcgccctt cgtcaccacg
     1021 acgaagttaa tgatgacgag aatggagaac accaccaagc cgaccgtgta attgcccccg
     1081 acgataaagt tcccgaaggc gcgaataact tctccggccg cccccgcccc ttcgttgcca
     1141 tgcagcaaaa tgagacgcgt ggaagcaatg ttgagggaca acctgaacaa tgtgatcatt
     1201 aaaaggaccg acggaaacac cgagaactcg atcggacgcc gcacttgaag tccgacgagc
     1261 aagatcacga ccgacaaggt gatatcgaag ctcaacaaca aatcgagcac aaatcgaggc
     1321 aacggcaaca gcatcaccat gagaatggcc actaccccga cggatatgac gacgtccgga
     1381 tgtttgatca actggtttcg ttccacgggc tctattgcag tcgccatcgc tgtctgatcc
     1441 taggaatttc ttagggaagt cacgcctctg gcgcgataca caaacgctaa gatctccgca
     1501 accgcgcggt atagctcatt tggaatctct ttgccgatat cgaccagttt gaacagcgtt
     1561 ctggccacga atttatgttc gacgaccggc acgccatgat gtcgagccag ctcgcgaata
     1621 cgttcagcga ccaagccggc gcctttggcg accacaacag gagctcccat cgtggcggtg
     1681 tcatatttca gagcaaccgc caggtgtgtc gggttggtaa tcaccacgtc ggccgtcttc
     1741 acggcagcca tcatgcgctt cttcgtcatc tcccgctgaa tggtccgcac acgactcttg
     1801 atgagcggat caccttccgt ggccttgtgt tcttccttaa tctcatcctt cgacatccgc
     1861 aggcttcgtt cccactcata gcgttggtag aaataatcga gcccggctag aaccgcgatc
     1921 gcccctgcga cggccaaact cactttcaag gagagacctc cagtaacttg gagcgcggtt
     1981 ccgaggtcga acccgatcaa tcccggaatc tggagcacgt cataccgtgc cacccaaagg
     2041 ccgatccccg tcacgatcgc gatcttgaga agccccttca cgagctccat cacagaacgc
     2101 agcgagaaca atcgagaaag ccccttgatg gggctgatgc gctcgacgtc cggctgaacg
     2161 gcattggcac gccacaacaa tccggtctgc aacagcgatg cagcgctccc catcaccaga
     2221 atgcctatga cgaccggaag actcagcgtc accactgtta aaccggcatg aatgacgacc
     2281 gagtacacct gctcgatcga cattccttcg tggaagtcca gggggaacga gagtgcgagt
     2341 ccttggcggg tcatctcggt cattttctga acgccgatcg gcaacatggc ccccagcaac
     2401 ccgatgccgc tcaagaggat cacagcgata gacagatctc gactgacggc gatctgccct
     2461 ttcttgcgcg cctcctcttt gcgtttcggc gtcgcgggtt ctgtcttatt actccggtcc
     2521 tcagccacgc cccagcgctt tcatgatccc atcgatcgtg aattgcagcc gctcgatttc
     2581 ccgaacaaga aactccacca caaacggtat ggacaatccc atcaccgcca agcctccggc
     2641 gatggtgacg ggaaaactca gcacgaacac gttgatctga ctgaccgcac gtccaagaag
     2701 cgcgagcaga atattgatca ggagaatgac gacgagcacg ggcgccgcca atttcaaccc
     2761 caccaagaac atgtgctgaa agaggcgaag gatatcctcc cccattcctc cggggaggga
     2821 cgcgccgaac gccggaatgg actcatagct ggagagaatc gtggccacta aa
//
LOCUS       DATMTI010001033         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_123041, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001033 DATMTI010000000
VERSION     DATMTI010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123041"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1534)
                     /locus_tag="VN039_16395"
     CDS             complement(<1..>1534)
                     /locus_tag="VN039_16395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV47578.1"
                     /translation="DGTYRWIEARASFVPEADGRSVRLLGSHTDITDRKRMEESVQES
                     EEWYRTLVELSPSGVFFFSEGRTVYVNHTGAVLLGAKKIQEILDRPTFEIIHPDYHQE
                     VRENVQRLLSGGVSVHSAERTYLKMDGTPIPVQVEAARITWNGKPAILGLFSDITERK
                     RGQDRVREMNLALAQAMPGISRVDLDGRYLEVNQPYATMLGYEPSELLGQTWEPTVHP
                     EDLCIAQHAYEQLLEKGKAEFECRAVRKDGSIFFTQVLMVSSKPSIGGAFGYHCFMRD
                     ITERKRTEKALADLNATLEQQVCDRTEALRQSEERFRQFFDHAPNVTALKDHNGRYVY
                     INRQFEKVCGFSPGIAIGKTDEELFSPEQAAQFQSHDQQVLLNGRGQEFEEVTHQEDG
                     FHTSIVVRFPVTDVLGRLSALGVIATDVTERKRTQEALRESEVRFQQFAESVGPAFWI
                     ADLVPDGETVVYANAAFTRLRGIAGEEWSQSVSRWLDSIHPDDRTRVEACRTRFISGG
                     LKA"
BASE COUNT          324 a          464 c          384 g          362 t
ORIGIN      
        1 ggctttcaac cctcccgaga taaaccgtgt gcggcaagcc tccactcgcg tccgatcgtc
       61 gggatggatc gaatcgagcc accgtgacac gctttgagac cattcttccc ccgcgattcc
      121 ccggaggcgg gtaaacgcgg cattggcgta cacgaccgtc tcgccgtccg gtacaagatc
      181 cgctatccag aatgccggac cgaccgactc tgcgaactgt tggaatcgga cttcgctctc
      241 gcgcaacgcc tcctgcgttc gcttccgttc cgtaacatct gtggcaatga cgcctagggc
      301 agagagacgg cctaaaacat cggtgaccgg gaatctcacc acgatactgg tatggaatcc
      361 atcctcctgg tgcgtcactt cctcgaattc ctgtccccgg ccattgagta acacctgttg
      421 atcgtgtgat tgaaactggg ccgcctgttc gggagagaat aattcctcat cggtcttccc
      481 tatggctatg cctggagaaa atccgcatac cttctcgaac tgtcgattga tatatacata
      541 acgcccgttg tgatctttca gagcagtcac attcggcgca tggtcgaaaa actgacgaaa
      601 ccgttcttca ctctggcgca gcgcttcggt ccgatcgcat acttgctgtt ccagcgtggc
      661 gttaagatcg gccaacgctt tctccgtccg ttttcgttcg gtgatgtcgc gcatgaaaca
      721 atggtagcca aacgcaccgc cgatggacgg tttgctactt accatcagga cctgcgtaaa
      781 gaagatggaa ccatcttttc gtacagctcg acactcgaac tccgcttttc ccttttccag
      841 caactgttca taggcatgtt gggcgatgca caggtcttca ggatggacgg tcggctccca
      901 tgtttgtccg agcaactcgg atggttcata ccccagcatc gtcgcgtagg gctgattcac
      961 ttccaggtag cggccatcta agtcaactcg ggaaatgccc ggcatggcct gagccaaggc
     1021 caggttcatt tcccgcacac ggtcttgacc ccgcttacgt tcggtgatat cggagaacag
     1081 gccgaggatg gcgggcttac cgttccacgt gatccgagct gcctcgacct gaaccggaat
     1141 cggcgttcca tccatcttca aatacgttcg ttccgcacta tggacggaca cgccgccgct
     1201 cagcagccgt tgcacattct cacggacttc ttgatgatag tccggatgga taatctcaaa
     1261 tgtcggccgg tcgagaattt cctgaatctt tttcgctccc aggagcacgg cgcccgtgtg
     1321 gttgacatag actgttcgtc cttcactgaa gaagaataca cccgatggtg aaagctcgac
     1381 caaagttctg taccattcct cactctcctg caccgactcc tccatccgtt tacgatcagt
     1441 aatatcggta tgtgagccga ggagacgaac actccggcca tccgcttcgg gcacaaagga
     1501 ggcccgtgcc tcgatccatc ggtaagtccc atcc
//
LOCUS       DATMTI010001034         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_166622, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001034 DATMTI010000000
VERSION     DATMTI010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166622"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VN039_16400"
     CDS             complement(<1..335)
                     /locus_tag="VN039_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWV47579.1"
                     /translation="MEGGMFVVIVLAVIVLIVIAKTAVVVPQQSAYVVERLGKYSSTL
                     DAGFHILVPFVDAIRYKHVLKENAVDIPEQVCITRDNVQVHVDGILYMRVLNPERASY
                     GISDYQFALT"
     gene            complement(362..790)
                     /locus_tag="VN039_16405"
     CDS             complement(362..790)
                     /locus_tag="VN039_16405"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWV47580.1"
                     /translation="MNWSYWLFMGLVLAGAEMISPGGFYLLFFGIAALIVGALAGLGI
                     VQTAWLEWLLFSFLAVVSLLLFRGPLLRMTKSTPTHVVDSMVGESAVLLDDLPSGHTG
                     KAELRGTTWTTRNDGASPLAKGQRAIVTKVDGLMLWVRSE"
     gene            936..2192
                     /locus_tag="VN039_16410"
     CDS             936..2192
                     /locus_tag="VN039_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HWV47581.1"
                     /translation="MAQDQPIKALVIALVDDAASAVYSINRLNPDTLCFVLPEGSKAL
                     VESDVQPKIQQMPRRWDWIVMADATAFPSTYQTIARSLPDLLRTWEIQPGELVVDLSG
                     ATAAMAGALSLVALPWTSRVVELVPARESAESDRVELGSKTFVWTQTNPWDEQGTVSR
                     REGCELFNRGLFHAAAKMFHDLELLVSGGQKPLYRAFRDLAEGYELWERFHYRQAWEK
                     LKTAIKALEMASLWGGPSGLKAVLPPIKANAGFLEKLVLDPAEVKEFVSLDLLAHAER
                     RLRAGHDPEAAMTALVRALEAFAQVRLYKLHKIKSWDVSPGQLPQALQETCRTCYLED
                     IDGKYKLPLQAQFRVLAGLGDQLGQAFLREWQKMKPLLDAANRAVLGHGFEPIKAERV
                     QQLHDVVVKLSGVNETSLPKFPVLTL"
     gene            2353..2751
                     /locus_tag="VN039_16415"
     CDS             2353..2751
                     /locus_tag="VN039_16415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HWV47582.1"
                     /translation="MEVKIYYEDTDCGGVVYYANYLKYFERARTEYLEMRGYSVAALM
                     KQGIIFVVVHAEVDYCSPGRYGETLVVETDVGDVTRAALTFSHVVREKVSGRVVVNGS
                     ARLAATDGNGKVKRLDGAMVAALQSASHPA"
     gene            2904..>3279
                     /locus_tag="VN039_16420"
     CDS             2904..>3279
                     /locus_tag="VN039_16420"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HWV47583.1"
                     /translation="MSQQTAIPGYTYGAAATSPVTVADFELMKKSALFGDEDVKYLRL
                     SHDIVKDQVEAILDVWYGFVGSQPHLLKSFLGKSDGKPLGDYLGGVRKRFGQWILDTA
                     CAEYDQKWLNYQHEIGLRHHRTK"
BASE COUNT          755 a          853 c          957 g          714 t
ORIGIN      
        1 gtgagcgcaa actggtagtc gctgattcca taagacgcgc gctccggatt aagaacccgc
       61 atgtacaaaa ttccgtcgac atggacctgg acattgtcgc gcgtaatgca cacttgttcg
      121 ggaatatcga cggcgttttc cttcagcacg tgcttgtacc ggattgcgtc gacaaagggc
      181 acaagaatgt gaaatcccgc atcaagcgtg gaactgtatt tccccaaacg ttccacgacg
      241 taggcgctct gttgagggac gacgacggcg gttttcgcga tcacgattaa tacgatcacc
      301 gccagaacta tgacgacaaa cattccacct tccatgccca cacctcgttt ggtagcattc
      361 gttattctga tctcacccag agcatcaacc cgtccacttt tgtcactatt gcgcgctgcc
      421 ccttggccag cggagaggcc ccgtcgttgc gggtagtcca tgtagtgccg cgcaattccg
      481 ccttacctgt gtgtcccgag ggaagatcgt caagtagaac ggcggattct cctaccatgc
      541 tgtctaccac atgggtcgga gtgcttttgg tcattcgcag caggggacca cggaaaagca
      601 ggagcgagac gacagccaga aaggagaaca gcagccattc cagccatgca gtctgcacga
      661 tacccagacc ggccagtgcg ccgacgatca gtgccgcaat gccgaagaac aggagataga
      721 atcctcccgg cgaaatcatt tctgcgccgg caaggactaa tcccatgaac aaccagtacg
      781 accagttcat cgaaaagcct cgttgtgcaa tagacactga tgagatctcg ggaatccttg
      841 cggagtctag ggctcattcg aatagccgtc aaggggagca catataatgg catcaaattg
      901 aacctccatg catgcgatga tatagtcgtc acgccatggc acaagaccaa cctatcaagg
      961 cgctcgtgat tgcattggtc gatgatgcgg cgtcggcggt ctattcgatc aacaggctca
     1021 acccggatac cttgtgtttc gtgttgcctg aaggaagcaa ggccttggtg gaatcggacg
     1081 tccagccgaa gatccaacag atgccgaggc ggtgggactg gatcgtgatg gcggacgcta
     1141 cggcattccc atccacctat caaaccattg cgcggtccct cccggatctc ttacggacgt
     1201 gggagataca gcctggggag ctggtcgtgg atctgagcgg ggcgaccgcg gcgatggcgg
     1261 gcgcgctcag cttggtggcg ctcccgtgga cttcccgagt ggtcgagctg gtccccgcgc
     1321 gcgaaagtgc ggagagtgat cgtgtcgagt tgggatccaa gacgttcgtc tggacgcaga
     1381 cgaatccatg ggatgagcag gggacggtgt cgcgccggga aggatgtgag ttgttcaatc
     1441 gaggcctgtt tcatgccgcc gccaaaatgt ttcacgactt agaactatta gtaagtggtg
     1501 gacagaagcc gttgtatcga gcattcaggg atttggccga gggctatgaa ttgtgggaac
     1561 gatttcatta ccgccaggcc tgggagaaat taaagactgc catcaaggcc cttgagatgg
     1621 cttcgctgtg gggcggacca tccgggttga aggcggtact accacctatc aaggcgaacg
     1681 cgggattcct tgaaaagctc gtgctcgatc ccgctgaagt caaagaattt gtatccttgg
     1741 atttgctggc ccatgccgag agaaggctcc gagcgggaca tgatcccgaa gcggccatga
     1801 ccgcactcgt tcgcgcgctg gaggcgttcg cgcaagtccg gctctataag ttgcacaaga
     1861 tcaaaagctg ggatgtgtcg ccgggacagc ttccgcaagc gcttcaagag acctgtcgca
     1921 cctgttacct cgaagacatc gacggcaagt acaaactgcc gcttcaagca cagtttcgtg
     1981 tgctggccgg gttgggcgat cagttgggcc aagcctttct cagagaatgg cagaagatga
     2041 aaccgttgct ggacgcggcc aaccgcgcgg tcttggggca cggcttcgag ccgatcaagg
     2101 ccgagcgagt gcaacaactc cacgatgtgg tcgtcaaact gtcgggtgtc aatgagacct
     2161 cgctgccgaa gtttcccgtg ttaactcttt aggagcgtat ggcttatggc atatagcagg
     2221 ggcagtaagc aggcgaagaa tggcctttcg cttcgacgcc tcgcgaaaag tatcaatgct
     2281 ttcagaaaca cgttgttgct gtcttaagca gtacttcttg ttcgtgccat aagccatacg
     2341 ctacaagctc ccatggaagt gaagatttac tacgaagaca ccgattgcgg cggggtcgtc
     2401 tactacgcca attatctgaa gtattttgag cgggctcgta ccgaatatct tgagatgcga
     2461 gggtattcgg ttgcggcctt gatgaaacag ggcatcatct tcgtggtcgt gcatgccgaa
     2521 gtagactatt gctcgccagg tcggtacgga gaaacgctgg tcgtcgaaac ggacgtaggc
     2581 gatgtgacgc gggctgcgtt gactttttcg catgtcgtgc gcgaaaaggt gagcgggcgc
     2641 gtggtggtga acggctcggc tcggttggcg gcgacggacg gcaacggaaa ggtcaaacgt
     2701 ctggacggag ccatggtcgc cgcgcttcag tcagcatccc atcctgcatg aactacctcg
     2761 atcctaaatg gtacagcacg ccgaagctga tcattttcaa gtgtcgccga cgatcatgta
     2821 accgtaccga tgcatcatcg tttggtacct tgacttgaaa cctacccgct gcgtagcatc
     2881 caaaaaaaga aaggagtgat gtgatgagtc aacaaacagc tattcccggt tacacttacg
     2941 gcgccgcggc tacgtctcct gtgacggttg ccgattttga gctgatgaag aagagcgcct
     3001 tgttcggcga cgaggatgta aaatatttgc gtctctcgca cgatatcgtg aaggatcagg
     3061 tcgaggcgat tctcgatgtc tggtacgggt tcgtcggatc ccaaccgcat ctgctcaaat
     3121 ccttcctcgg caagagcgac ggcaagccgc tcggtgatta cttgggcggg gtgcgtaagc
     3181 gattcggtca atggattctt gatacggctt gtgcggagta cgaccagaaa tggctcaact
     3241 atcagcacga gatcggcttg cgccatcatc gtacgaaga
//
LOCUS       DATMTI010001035         1540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_209956, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001035 DATMTI010000000
VERSION     DATMTI010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209956"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VN039_16425"
     CDS             <1..257
                     /locus_tag="VN039_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47584.1"
                     /translation="LSRDIDLESYYFPRAYVVRPVRLADETVPLNGPMSMTMRATDAV
                     GTMRSTLVIGSKDVRKLQHDDMAMSAFRRGKVIIVMDPLR"
     gene            complement(292..>1540)
                     /gene="tssJ"
                     /locus_tag="VN039_16430"
     CDS             complement(292..>1540)
                     /gene="tssJ"
                     /locus_tag="VN039_16430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HWV47585.1"
                     /translation="YVVDEQGETVFIAGADGMTTVGAIPMSNDTTLVESLTTLLSRSN
                     NASALASVDNPSSKIQLSARVASEATARGLTLSSSEILNPDRNGKPLSLVVRLYQLSG
                     KEKFEQATYQALLKGDHKVLEGELLSKSEVTLHPNSREVTKIDPGKDALYIGIMALFR
                     EPQAQRWRQIIPVRDAAAQSLDVAFEGSGILGVSGEPLMAKRGVKLVADTSAPRVRIK
                     KNNEPRTAENSLQLAIQANCDCYITIVDVDAEGGVNLLFPNDAQGPKFYPKGKVRSGE
                     TVLVPDSLNEHNKAGFHFDYSTPPGVDTIRVFASTDLDTAEMIRRYAKNPAQVPGPKL
                     DAERMVFQPIIVASATLDELKSGLRTRGVRVTKSTPQAKPGTDTHSAATGGVSALGEG
                     SVVPVSFQGSDWTATSLTVVVDP"
BASE COUNT          307 a          433 c          431 g          369 t
ORIGIN      
        1 ctttatcgcg ggatatagac cttgagtcgt attattttcc ccgcgcctac gtggtgcgac
       61 ctgttcgcct ggctgacgag acggtaccgc tcaacggccc gatgtcgatg accatgcgcg
      121 ctaccgacgc cgtcggcacg atgcgatcca cgctggtgat cggttccaag gatgtccgta
      181 agttgcaaca cgatgacatg gccatgagcg cattccgtcg tggcaaagtc atcattgtga
      241 tggacccact tcgatagatg aaacagccgc tcatggggat ttccgccagc gctacggatc
      301 tacgacaacc gtaagcgatg tcgccgtcca gtccgagcct tgaaaactca ccggcacgac
      361 tgatccctct cctagggcag aaactcctcc tgtagctgcg ctgtgtgtat cggtccctgg
      421 ctttgcctgt ggcgtactct tcgtgaccct cacgccacgt gtgcggagac cggatttcag
      481 ttcatcaagt gttgccgatg cgacaatgat gggttggaag accattctct ccgcatccag
      541 tttaggtcct ggtacttgag ccggattttt ggcatagcgg cggatcatct ccgctgtgtc
      601 aagatccgta ctcgcgaata cgcggatggt atcgacaccg gggggcgtgg agtaatcgaa
      661 atggaaaccg gccttattgt gctcattcag actgtcgggg accagaacgg tttcacccga
      721 tctcaccttt ccctttgggt agaacttggg tccctgcgcg tcgttgggaa agaggagatt
      781 gacgccgcct tcagcatcga cgtcgacaat ggtgatgtag caatcgcaat tggcttgaat
      841 ggccagttgc agactgttct ccgccgtgcg cggctcgttg ttcttcttga tccgcacccg
      901 gggtgcactc gtgtcggcta ccaacttgac cccgcgcttg gccatgaggg gttctcctga
      961 tacgcccaga atgccgctcc cttcgaatgc cacatcgagt gattgagccg ccgcgtctct
     1021 caccggaata atctgccgcc accgttgagc ttgtggctca cgaaacagag ccatgattcc
     1081 aatgtacaag gcatccttcc ccggatcgat cttcgtgacc tctcgggaat tcggatgcag
     1141 agtgacttca cttttcgaga gcagttcacc ctccaggact ttgtgatcac ctttcagtaa
     1201 ggcctgatag gtggcctgct cgaatttctc ctttcccgac aattgataca gacgtacgac
     1261 gagcgagagg ggtttgccgt ttcgatcggg attcaatatt tccgacgatg aaagcgtgag
     1321 gccgcgggcg gtggcctcac tcgccacgcg ggcgctgagc tgtattttgg aagaggggtt
     1381 gtcgacagaa gccagcgcgg aagcattgtt ggatctcgaa agaagagtgg tcaggctttc
     1441 tacaagggtc gtatcgttgg acatggggat agcacccacg gtcgtcattc cgtccgcacc
     1501 cgcgatgaag acggtttccc cctgctcatc gacgacatat
//
LOCUS       DATMTI010001036         1965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_675675, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001036 DATMTI010000000
VERSION     DATMTI010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675675"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..1837
                     /locus_tag="VN039_16435"
     CDS             380..1837
                     /locus_tag="VN039_16435"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWV47586.1"
                     /translation="MNTPTWDEFAELALKRIAKSGAEYGDIRIQDSSTEQIEGEDRRI
                     AAVRDVRDIGFGVRVLYHGAWGFAASSILSMEEVPRVADLAVEIAKGSASVALEKVRL
                     APEPVHRDRVVTPYRIDPFSVSLEKKTDLLLNTMEVLQRQSGIARSSASLWARRDRKL
                     FVSTEGSHLEFDLLAGQGECTATALHEGRFASRSFNTPHLRKGFELIEEADFLREASR
                     VADQAIEKVKAPAVDAGQYDLVLDPEHLSLTIHESCGHPTELDRALGYEANYAGTSFL
                     TTEKRGNFRYGSRHVNLVADNTEPETLAATGYDDDGVACQRWDIIRHGIFVGYCTNRE
                     VATKIGESRSCGSNRADGWGNIPIVRIANIGLEPGEAPRDQLIADVKRGVYIEGHGSY
                     SIDQRRYNFQFGGDAFWLIENGRRTHMLRDVIYHGITPEFWNSCDGVADRSFRRRYGF
                     ITCGKGQPGQSGWMTHAAAPARFRQVHVIRGEGSS"
BASE COUNT          415 a          524 c          581 g          445 t
ORIGIN      
        1 tcattcgttt tgaagcggcg catgggtaag tgcttctcgc agtgtaactt tgagcgtctc
       61 aactagagct tcgcggctat gttcttggca attcacaccc ggtacttctt cgatccaccc
      121 tatccatcac ccggcgtctt gtttgatcac cagctgtgta gtttcgctgc atgagcgccc
      181 tcctctgtac cggcagacgc aagctcagcg tcctgttcaa agagaagttt cactgccgag
      241 gtcaagaggc tacagctatc actacggcct gtcggcagta atgccaaact cgttcgttcg
      301 atcccgacag tttcaccgct ttgctcgcct cctgttatac taacccgatt catcattcgt
      361 tgactgtgag gatcgctgta tgaacactcc aacgtgggat gaattcgccg agcttgcgtt
      421 gaagcgcatt gcgaagtcgg gcgccgagta cggcgacatc cgcattcagg acagcagcac
      481 ggagcagatc gaaggcgaag atcgtcggat tgccgccgtt cgagatgtca gggacatcgg
      541 cttcggcgtg cgagtgctct atcacggagc ctgggggttt gccgccagtt cgatcttatc
      601 gatggaagag gtaccacggg tggcggacct ggccgttgag atcgccaaag ggtctgcatc
      661 ggtggccctc gaaaaggtgc gtttggcacc ggagccggtc catcgcgatc gcgtcgtcac
      721 gccgtatcgc atcgatccct tcagcgtgtc gttggagaaa aaaacggact tattgctcaa
      781 taccatggaa gtgttgcaac gacaatccgg cattgcaagg agcagcgcaa gtttgtgggc
      841 gcgtcgggac agaaagttgt ttgtctcgac cgagggatca catttggaat ttgatctcct
      901 cgcaggacag ggggagtgta cggcgactgc gttgcatgaa ggccgatttg cgtcgcgcag
      961 cttcaatacg ccgcatctca ggaaaggctt tgagctgatc gaagaagcag atttcttgcg
     1021 ggaggcttca cgtgttgccg atcaagcgat cgaaaaggtg aaggcgcctg cggtcgacgc
     1081 cggccagtat gatctggtgc tcgatcctga acatctgtca ttgaccatcc atgaatcctg
     1141 cggccatccg accgaactgg atcgagcgct cggctacgag gccaattatg ccggtaccag
     1201 tttcttgacg acggagaaac gtggcaactt tcgctatggc tcgcggcatg tgaatttagt
     1261 tgccgataac accgaaccgg aaactctggc cgcgaccggt tacgatgatg atggagtggc
     1321 gtgccagcga tgggacatca tccgccacgg gatctttgtc ggatactgta cgaaccgtga
     1381 ggtggcaaca aagatcggcg aatctcgttc atgtggctcc aatcgggctg atggatgggg
     1441 gaacattccg atcgtgcgga ttgccaatat cggacttgag cccggcgagg cccctcgtga
     1501 tcagcttatt gccgacgtga aacgcggcgt ctatatcgaa ggtcatgggt cctacagcat
     1561 cgatcagcgt cgctacaatt tccagtttgg cggtgacgcg ttctggctga ttgaaaacgg
     1621 caggcgaacc cacatgctgc gggacgtgat ttatcatgga atcacgccgg aattctggaa
     1681 cagctgcgac ggagtggcgg accgatcatt tcgccggcgg tacggcttca ttacctgtgg
     1741 gaaggggcag ccgggtcaat ccggctggat gacccatgcc gccgctcctg cgagatttcg
     1801 ccaggtccac gtgatccgag gagaaggaag ctcatgacca tgtcgagcgg cacttggccg
     1861 aagatgacca cctccgacga gtttcgtttt ctgagtgagc tggtcatgac ccgctcatcc
     1921 gccgatcata cgatcgtgag gcttcgcgat caccatgctg gaacg
//
LOCUS       DATMTI010001037         4275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_590337, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001037 DATMTI010000000
VERSION     DATMTI010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590337"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VN039_16440"
     CDS             <1..540
                     /locus_tag="VN039_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47587.1"
                     /translation="EKKRKIANHTFPGRIGKLRQEAAKLDVQTKVDGDKTIVMVKVQS
                     LVPHNLPATHPAWATVVLNLDIKGKNLKTVFSDKRVYGRTYLDAQGQKTVFDFEAAKV
                     AEDTVLKPEETREETFTFPTPKDTKTFDVEVGLNYGPLSGPASFLERVEAESSKGSQD
                     PVFQPIEIVKRTENIPVGK"
     gene            1016..1255
                     /locus_tag="VN039_16445"
     CDS             1016..1255
                     /locus_tag="VN039_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47588.1"
                     /translation="MKESARYAKIVEWSEEDQCYVGSSPGLIYGGCHGPDEKAVFDEL
                     CQIVEEAIAIYHKDGKPLPPSTSGRDFANKMQDVA"
     gene            1889..3250
                     /locus_tag="VN039_16450"
     CDS             1889..3250
                     /locus_tag="VN039_16450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV47589.1"
                     /translation="MQQRIRRGTKVIVVGAGLAGLTAAVRLKQMGAKVSLIEARDRVG
                     GRVLTIRGAFAQGQHAEAGADFIDEGQHEIKRLIEEQGLTLRPILRQGFAFIRHPGPG
                     RKRRHILSGEEAWDSLAHALCPLVAAYRAADKRWDSPIAGQLARQSAAQWLEEVKADS
                     NMRAVVRGLRGFFLADPEELSLLALVDQLAGDVPGQTGMYRIEGGNDRLPLALAEHLR
                     EDLRLNSVVRAISQDRNSVRVRVETSNGQESSIQGKYVVLAVPTPTLRSIHMQPPLPA
                     MQARAICSLKFGRTTKALLQFDKSFWRKKGRPLACGTDAPTGAFWDGNEEQAGEAGIL
                     TMMAGGQASEDTQKIVAQRGVEGLVDALDWMGSRSATLLHSRIVTWEDDPWAQGGYAY
                     FDPGFDPHLRDWLARPHGRIIFAGEHTSVTWQGYMNGAVESGLRAAEEVCAMVLKKCE
                     HTA"
     gene            complement(3302..4006)
                     /locus_tag="VN039_16455"
     CDS             complement(3302..4006)
                     /locus_tag="VN039_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47590.1"
                     /translation="MSYTYDYTKATAGESASRRYWRLMKMTLTRLVRVKTFRFDGKTY
                     EYLYHMYNKTWKNERGIEIPIFRELLMRYQNKRILELGNVLAHYCPVHHDVVDKYEVA
                     PGVINEDVVEFIPQQKYDLIVSISTLEHVGWDEQPREPDKLLRTIEHLRERCLAVGGT
                     MMASLPLGYNTYFDELIENGQSPFTTQYFLKRISKKNYWIESDWEGCRTSPYGRSVAN
                     ALYIGIIRGESVSTTG"
     gene            complement(4027..>4275)
                     /locus_tag="VN039_16460"
     CDS             complement(4027..>4275)
                     /locus_tag="VN039_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47591.1"
                     /translation="FEPNYIIIDGQTFTKAKLSLDNHVYKNCQIDDCDLYFSGGQYEL
                     LDTHITNSRLILNHPAKGIYNALQIFKMKSPGSRIIFE"
BASE COUNT         1033 a         1087 c         1228 g          927 t
ORIGIN      
        1 gaaaagaaac gcaagatcgc aaaccatacg tttcccgggc gcatcggtaa gttgcgtcaa
       61 gaagcggcga aactggatgt acagaccaag gtcgacgggg acaagacgat cgtgatggta
      121 aaggtccaaa gcctggtacc gcataatttg cctgctaccc atccggcttg ggccacagtg
      181 gtcttaaatc tggacatcaa agggaagaat ctcaaaacgg tcttttctga taagcgcgtc
      241 tatggtcgga cgtatcttga tgcccaagga cagaagacgg tttttgactt tgaagcggca
      301 aaagtagccg aagacacggt cctaaagccc gaagagacga gagaagaaac ctttaccttc
      361 ccgacgccga aagacaccaa gacatttgat gtcgaagttg gactaaatta tggccctctc
      421 agcggccccg cctctttcct cgaacgtgtc gaagccgaat cctcaaaggg atcgcaggac
      481 ccggtattcc agccgattga gatcgtcaag cggacagaaa atattccggt cggcaaataa
      541 gtcggcgccg gtcgatcaca cgatacactt gcctcgctga gtgggcaaag cggacttcac
      601 gagcgatgtg cctctgtggg atgttgccgg aaccgtcatg aacaatgccc agtgaagtaa
      661 ggagactggc tccggcacag ccgtcgcctg tcccggtctg tcctcccttg acaagtcctt
      721 gggaagacaa gagaattgct aaactttctg gaatgtaatc gcaataatat cctgaccaaa
      781 aaactcacgg attatgcgag gctcaaacga atggagatcg attgccgccc aagattcgcg
      841 agctgatttc cgagctgcag aaggctggtt ttaccgaccg tggtggcaag ggaagtcacc
      901 gaaactttgt gcacccaaag gcgacaaaac cgatcacaat atctggagcg ccaggcgatg
      961 acgcgaagca ttaccaagtt cgggcagtcc gccgtgcaat tgaggagtca aagtcatgaa
     1021 ggaaagcgcg cgttacgcaa agatcgttga gtggtccgaa gaggaccagt gctacgtcgg
     1081 cagttctccc ggcctcatct atggcggttg tcacggcccc gacgaaaagg cagtgtttga
     1141 tgagctttgc cagatcgtcg aagaggccat cgctatttac cataaagacg gtaaaccgct
     1201 tccgccgtcg acatccggtc gggattttgc caacaagatg caagacgtcg cgtagcaacg
     1261 tcctgtgcag atcctcggcg acagcggcac agatttatgg ggtctgccca atcgaacgtt
     1321 ccttacgaag atcagccgtc cagcactgtt gaagcagcca aagagacact gtctggccag
     1381 gtcgaagcgc ttcgatgcac atttgacaag gtgttcaagg atggtgcggt caaacccatc
     1441 gttacacccg atgtgctata gcgggcttcg cccgcttccg cacgcggatg aagtccaacc
     1501 ttcggcaacg gaggtcgagt tgcggtgaag aaggcgcaca ttctcgagga gatcaagcgc
     1561 aaagaagaca gaggtggggt tggatcttgt actgtgcatc accaaattgt gagtctcctc
     1621 cagtgttcca gcaatccagt cagcgctaag gacttggcat cagtattgac ttacatcatt
     1681 catgagcatt gctgcttcaa tcgccgatac gccgatatcc gtcattcgtg aagcccgctt
     1741 ctccgagtta ctctcgctga gatgatgcga gcgatgtctc acagacatgc tctctccaaa
     1801 cccctaggga taccgatcat ggaattccag tacctgccgg ggctagactg actcctaggc
     1861 tcctccgctg cttatgccaa tgaaggttat gcaacaacgg atacgccgag gtacgaaggt
     1921 cattgtcgtg ggagcaggct tggcaggtct gactgccgcg gtgaggctca agcagatggg
     1981 ggcgaaagtc tcgctgattg aagcccgcga tcgagtcgga gggcgcgtcc tcacaatcag
     2041 aggcgcattt gctcaagggc aacatgccga ggcgggggcc gacttcatcg acgaaggcca
     2101 acatgagatc aaacgattaa ttgaggaaca gggactgacc ttacgcccaa ttctgcgaca
     2161 gggtttcgcc ttcattcgcc atccaggtcc tggccgtaaa cggcgacaca ttctgtctgg
     2221 tgaggaggcc tgggactcat tggcccatgc gctctgtccg ctggtcgctg cctatcgagc
     2281 agccgataag cgatgggaca gtccgattgc cggacaactg gctcggcaat cagcggccca
     2341 atggttggag gaggtcaagg ccgacagcaa catgagggcc gtcgtgcggg gcttgcgcgg
     2401 atttttcctg gccgatccgg aagagctttc cttgttggcg ctcgtggatc aactcgccgg
     2461 agatgtgcct ggacaaacag gcatgtatcg aattgaaggg gggaacgacc ggcttccgct
     2521 tgcccttgcc gagcatctac gggaggatct gcgactcaat agcgtggtgc gggccatttc
     2581 gcaggaccgc aattcggttc gagtgagggt tgaaacatcg aatggacaag aatcgtcgat
     2641 acaaggaaaa tatgtcgtgc ttgccgtgcc gacccccacg ttgcgctcca tccacatgca
     2701 gcctccgcta ccagctatgc aagcccgcgc catttgctct ttgaaattcg gaaggaccac
     2761 gaaagcgctt ttgcagttcg acaaatcgtt ctggagaaag aaaggaaggc ccctggcatg
     2821 tgggacggat gctccaaccg gcgccttttg ggatggcaac gaagaacaag cgggtgaggc
     2881 cggaattcta accatgatgg ccggtggaca agccagcgag gatacccaaa aaatcgtggc
     2941 gcagcgtggg gtggagggct tggtggacgc attggactgg atgggaagcc gatcagctac
     3001 tctgctccat agccgaatcg tgacctggga agacgatcca tgggcacagg gaggctacgc
     3061 ctattttgat cctggcttcg atccccacct gcgcgactgg cttgcccggc cgcatggacg
     3121 gatcatcttt gcgggagaac acacgagcgt gacctggcag ggctacatga atggagctgt
     3181 ggaaagcggg cttcgagctg ctgaggaggt ctgtgcgatg gttcttaaga agtgtgaaca
     3241 tacagcatga ctcgtaagcg attcagtcga aagcgtggct ccaacgacac ttcagagggc
     3301 ttcatccggt agtagaaact gattctcctc ggatgatgcc gatataaagg gcattcgcga
     3361 cggatcgtcc atagggtgaa gtacggcagc cctcccagtc tgattcaatc caatagttct
     3421 tcttggaaat ccgcttgaga aaatactgcg tggtgaacgg agactgcccg ttctcaatga
     3481 gttcgtcgaa gtaggtattg tatcccagcg gcaagctggc catcatggtg ccgccgacgg
     3541 caaggcatct ttctcgtagg tgctcaatgg tccggagaag tttgtccggc tcacgcggct
     3601 gctcatccca cccgacatgc tccaaagtcg agatgctgac gatcaaatcg tacttctgtt
     3661 gagggataaa ctcgacgacg tcctcgttta tgacaccagg agcgacctca tacttatcga
     3721 cgacatcgtg gtgaacagga caatagtggg ctaggacgtt gccgagttcg agaatccgct
     3781 tgttttggta tcgcatcaaa agttcgcgaa agatcgggat ttctatcccc cgctcgtttt
     3841 tccaggtctt gttgtacatg tggtacaggt actcgtacgt cttgccgtcg aaacggaagg
     3901 tcttgactcg cacgaggcgg gtcagcgtca tcttcatgag gcgccagtat cgcctggagg
     3961 cgctctcacc ggctgtcgcc tttgtgtaat cgtaggtata ggacaagaag ggcctgcttc
     4021 gagggattac tcgaagatga tgcgagatcc gggtgatttc attttgaaga tctgtaaggc
     4081 gttgtaaatg cccttggccg ggtggttcag gatcaggcga gagttggtga tgtgagtgtc
     4141 gaggagttca tactgcccac cactaaaata aagatcgcag tcgtcgatct gacagttctt
     4201 atagacatga ttgtccaagg aaagttttgc cttggtaaag gtctgtccat caatgatgat
     4261 gtagttcggt tcaaa
//
LOCUS       DATMTI010001038         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_166697, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001038 DATMTI010000000
VERSION     DATMTI010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166697"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VN039_16465"
     CDS             complement(<1..823)
                     /locus_tag="VN039_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWV47592.1"
                     /translation="MLLSLALIVPFLLLTAACIVMIGDETSRFTRAKVAAYPIGLAFL
                     GAICTLTLVTSQGPVTLRFYDPASVASFIIPIGFHVDRLSAVMMTLITGVSTIIYCYS
                     TSYMYQDRHARRYLALICLTDFVLICMVSSGNLMMLFLFWQLLSYLLYLLAHNHVHAA
                     TLAGAFKTFTLLRVSDTAFLAGIVLAYQLYGTLEFHELFARAAATPMTLSILPGMDVS
                     ATTAVTFLLFIGAMGKSAQFPLHLWLPGSLFAPTPVHALLHAGIINAGGFLINRLA"
     gene            complement(1043..>1673)
                     /locus_tag="VN039_16470"
     CDS             complement(1043..>1673)
                     /locus_tag="VN039_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWV47593.1"
                     /translation="ARQEPVLPHPGHEAEEESYSPLTWTTGVLTTLLIPLLILLATHG
                     VLRIPLLESQGTVIFLFFIWITSSQAILTLTRLRAVASWKVSAVMLLTLLFIVFVYLF
                     AVESFTAFLYPNPEEVASYFKAADLPDWLFDVLLVVSTLVTIAGWIYLYMRAHGRTVW
                     TPSWIDSVRIRLYVLFLNRLYVDAVLHRVEHTLTSVIQRLDKHAQERSL"
BASE COUNT          437 a          426 c          521 g          289 t
ORIGIN      
        1 gagccaggcg gttgatgaga aaacctccgg cgttgatgat accggcgtgc agcaatgcgt
       61 ggaccggcgt gggcgcgaag agcgaccccg gcaaccacag atgcagagga aactgagctg
      121 atttgcccat cgcgccgata aaaagaagaa aggtcaccgc cgttgtggcg ctgacatcca
      181 tcccgggcag aatcgaaagg gtcatgggtg tggccgccgc tctggcaaac agctcgtgga
      241 actcgagggt gccgtacagt tgatacgcga ggacaatacc ggcaagaaac gcggtatcgg
      301 agacgcgcaa caacgtaaac gtcttgaacg cacctgccaa cgtcgcggca tggacatgat
      361 tgtgcgcgag cagatagagg aggtaactga ggagttgcca aaagagaaac agcatcatga
      421 ggtttccgct tgaaaccatg cagatcagca cgaagtccgt cagacaaatc agcgccagat
      481 accggcgcgc gtgacgatcc tggtacatgt agcttgtcga gtagcaatag atgatggtgc
      541 tgacacccgt gatcaacgtc atcatgactg cgctcagccg gtcaacatgg aaaccgatgg
      601 gaataatgaa ggaagcgacg gaagcaggat cgtagaatcg aagggtgacc ggaccctgcg
      661 aggttactag ggtcagcgtg cagatagccc cgaggaatgc cagaccgatg ggataggcgg
      721 cgaccttcgc gcgagtgaaa cgcgaggtct catcaccgat catcacgatg caagcggcgg
      781 tcaggaggag aaatggaacg atgagtgcca gcgacaggag cacggtattc cttagtaggt
      841 agttgaaaaa atccgccagc ggcgttcaca aacgtgccgc gccttactcg gcgcggcgta
      901 aacctcagtc aggtgatggg tacacctccc acgatggtgc aatgatggga ggctaccatt
      961 aatactggtt ctccacattc gtcaagcagg gacggatgag gagcctatgt cgaacagctc
     1021 tgtgtcgtca agccagcgtc ggtcaaagcg aacgttcttg ggcgtgctta tccaagcgct
     1081 gaatgacaga tgtcaaggtg tgttccactc gatgtaagac cgcgtcgaca tagagccgat
     1141 tgagaaacag cacatacagg cggatccgga cgctgtcgat ccacgatggc gtccatacgg
     1201 tccggccatg ggccctcatg tacaggtaga tccagcctgc gatcgtcacc agcgtcgata
     1261 ccacgaggag gacgtcaaag agccagtccg gaagatcggc ggccttgaag taggaggcaa
     1321 cctcttccgg gttagggtac aggaatgcgg tgaacgattc gacggcgaac agatagacaa
     1381 acacgatgaa caggagggtc aacaacatca ccgccgatac cttccaggag gcgacggccc
     1441 gcaatcgggt cagcgtcagg atcgcttgcg aagacgtgat ccagatgaag aacagaaaga
     1501 tgacggtgcc ttgagattcc aacagcggaa ttcgcaacac gccgtgagtg gccagcagga
     1561 tgagtaaggg aatcagcagg gtcgtgagaa ctcccgtcgt ccaggtcagg ggcgagtagc
     1621 tttcttcctc cgcctcgtgt cccggatggg gaagtacggg ttcctgtctt gcc
//
LOCUS       DATMTI010001039         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_633046, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001039 DATMTI010000000
VERSION     DATMTI010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633046"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..822
                     /locus_tag="VN039_16475"
     CDS             232..822
                     /locus_tag="VN039_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47594.1"
                     /translation="MMREGKSMIMPAGALIMAVTAALLPSSVLGEGGARRDVVRQAAE
                     STGAERAAPTSLSKHATIMSREGQVVRKGSNGWTCMPDNPGTTGTDSICMNEPWLTFM
                     DAMKQKKDPVIAQVGIAYMLQGDRPTSNTDPYATEPKTGDDWVEGLGAHMMILLPDQQ
                     VLKNVPTDSKNGGPWVMWAGTPYAHLMIPIDSYPSR"
     gene            complement(871..1317)
                     /locus_tag="VN039_16480"
     CDS             complement(871..1317)
                     /locus_tag="VN039_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47595.1"
                     /translation="MTFTMDTMIRLLRIANIAMLLLYILGSFLRVSAEASRLSKGGFV
                     PLTAFLIVALLVFTLHVLSLSYAWRGLSSTSTLRVVRIAWGLNIVLVLFSLYQIVGSL
                     LDKTEDFLSNPFFTLLVVLNVVALGKRKSMLNAMPAIQGTDTLPTK"
BASE COUNT          389 a          398 c          473 g          350 t
ORIGIN      
        1 gagtcttcaa tctgcggtga ttcggaagcg gatgtgaaaa ggccgcgtct caggtgacgc
       61 ggcctttgta tttccggcag ccctctactc gttcgtccgg cgtcgcatgt cggatcgtgc
      121 cgatcctggt tcacgtccat cgcgtcgtgc ctttttcatt gcactccaaa tcttcgagcg
      181 cttaggatat cgacgcatga tcgtcgtcct attgagaaag gagagatgcc tatgatgcga
      241 gaggggaaat ccatgattat gccggccgga gcgttgatca tggccgtaac cgccgcactc
      301 ttgccgtcgt cggtcctcgg tgaaggaggg gcgcgccgtg acgtggtacg tcaagcagcc
      361 gaatcgacag gtgctgaacg ggccgcgccg acgtcgttgt cgaaacacgc aacgatcatg
      421 agccgagagg gacaggtcgt tcgcaaaggc agcaatggat ggacctgcat gcccgacaat
      481 cctgggacga cgggcacgga ttcgatctgc atgaacgaac cttggctcac cttcatggat
      541 gcgatgaaac agaagaagga tcctgtcatt gcccaagtcg gcattgccta tatgttgcaa
      601 ggcgacaggc cgacgagcaa tacggacccc tatgccactg agccgaagac gggagacgat
      661 tgggtggaag gacttggtgc gcatatgatg attctcttgc cggatcagca ggtgctgaaa
      721 aatgtgccga ccgattcaaa aaacggcgga ccatgggtca tgtgggccgg aactccctat
      781 gcccatttaa tgattccgat cgacagctat ccatcacggt aggtcgggat ggggagacga
      841 ttcggtttgt agcacaggat gcggacagct tcacttggtt ggtagagtgt ccgttccctg
      901 gatggccggc atcgcgttca acatggattt gcgttttccc agcgcaacga cattgagtac
      961 gaccaagagg gtgaagaaag gattagaaag aaaatcttcg gtcttgtcca gcagggaacc
     1021 gacgatctga tacaggctga acaggaccag gacgatgttg agaccccatg cgatccgcac
     1081 aactctgagc gtactggttg aacttaaacc acgccaggcg taactcagcg atagaacgtg
     1141 tagagtaaac acgagcaacg cgacgatgag gaaagcagtc agcggtacaa agcctccctt
     1201 cgataatcgg ctcgcttctg ctgaaactcg gaggaagctt cctagtatgt acagaagcaa
     1261 catcgcgatg ttggcaatgc ggagcaggcg aatcatcgta tccattgtga aggtcacaga
     1321 catcctttca attcgagcag ttgatcggct gagaacttgt ttgagttggt ggagatccag
     1381 gactggacaa ggcgtgagga atcgagccgt ccaactgttg ccgctcggct ctgaatcctg
     1441 actcccgacg tctgccgatc atacgggttc cggtcgaaca acagaaacgg attcattgga
     1501 agtgtttatc ccgtcgccaa gaataatcat aagggcttct gatcgtggca agcaagggat
     1561 ggagctgggg gtaggcgtct atatccctag tccttaagtt tcgtctagat
//
LOCUS       DATMTI010001040         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_590399, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001040 DATMTI010000000
VERSION     DATMTI010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590399"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VN039_16485"
     CDS             complement(<1..959)
                     /locus_tag="VN039_16485"
                     /inference="COORDINATES: protein motif:HMM:NF042623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HWV47596.1"
                     /translation="MATFSRNKRKPFYTRLVIPTRLRVYFQGKREVWRSLGTKDKAVA
                     ATRALQFEAQGRRLFLTLSRRGNRMTRYEIETLVTRWLDEALDEAEDDRATLGPITEA
                     AREDIQDGLSATFDGTTEALLSGDYRTISKEANDLLHVAGLPALDHGSQDFARLCRRL
                     LRAKVEYLRLEGERWNGEYREATVSPRDKLPVLVKAKPHVSLAFSVVLDKYLSANPRP
                     ARTANPLRAEFMRFIETIGGDKPIAAITKADVVAYKEVLQTQRKISLLTCAKHLSNLD
                     ALFKWACTHDYLDQSPARGLAPSKRQAKKHALQRRPFTDDELLT"
     gene            complement(1069..1145)
                     /locus_tag="VN039_16490"
     tRNA            complement(1069..1145)
                     /locus_tag="VN039_16490"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1109..1111),aa:His,seq:gtg)
     gene            complement(1209..1922)
                     /gene="pyrF"
                     /locus_tag="VN039_16495"
     CDS             complement(1209..1922)
                     /gene="pyrF"
                     /locus_tag="VN039_16495"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWV47597.1"
                     /translation="MVKIAARDRLIFALDVPSTVEADRLLDRLQGHISFVKVGLELYT
                     AAGPAMVQRIVERGMRVFLDLKFLDIEETVRRATARVATMGVDFLTIHANRKALTAAV
                     QGKEGSSLKLLAVTVLTNFDSNDLRDMGIQRTVQDLVTARALLASEVGCDGVVASGEE
                     AAAIRQKVGPKFAVVTPGVRPAGKGIDDHARATTPTQTIASGADYLVIGRPIRDAVDP
                     AATVAAILVEMQAAFDARG"
     gene            2007..2285
                     /gene="rpsT"
                     /locus_tag="VN039_16500"
     CDS             2007..2285
                     /gene="rpsT"
                     /locus_tag="VN039_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWV47598.1"
                     /translation="MPRVHKSTIRRARQADRRHERNRATLNSVKTLIKKVQSAVAGKK
                     ADEAKTSLLEATSAIGKAVSKGVLHRNTASRRISRLALCVNALSGSGS"
     gene            complement(2257..>3027)
                     /gene="holA"
                     /locus_tag="VN039_16505"
     CDS             complement(2257..>3027)
                     /gene="holA"
                     /locus_tag="VN039_16505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWV47599.1"
                     /translation="VSPKLDGRLKFSKALARAAIVIDCSPLRDAQLPAWIAREAERVG
                     LRLEEAASQVLQESCGSSLYGVRRELEKLASYIPSDRSVTAHDVHLLRGMDPGASVFD
                     LTLAIAEGRRGRALSILARNLEAGEAPLRILGSLAWQYRRLWKIKDSLANGGREGEVA
                     RGLRMDPWKVRSFLDRFSEPHLQAALRLFLDTDGRLKGGSGSRPRIVLERVLLKLCED
                     TADRRAEPAPCPPTSPKRVPARVVSNVRTIKSRNRTGH"
BASE COUNT          660 a          909 c          815 g          643 t
ORIGIN      
        1 gtcagtagtt cgtcgtcagt gaacggtctc cgttgcaggg catgcttctt agcctgtcgt
       61 ttactggggg caagtcctcg tgccggggac tgatcgaggt agtcatgggt acaggcccac
      121 ttgaagaggg cgtcaaggtt cgatagatgc ttggcacagg tgagtaggga gatcttccgt
      181 tgagtctgca acacttcctt gtaagctacc acgtcggcct tggtgatggc cgctattggc
      241 ttgtcccctc cgatggtttc aatgaacctc atgaactcag cccgaagagg gttagctgtt
      301 cgagcaggcc gagggttagc cgagaggtac ttatcgagga ctacagagaa ggccagagac
      361 acatggggct ttgccttcac caataccggc aacttgtccc ttggggagac ggttgcttcc
      421 cgatactccc cgttccaccg ctcaccctct agcctcaggt actcaacctt ggccctgagg
      481 agtcgtctac acaatctagc gaagtcctgg ctaccatgat ccagagcagg caggccagcg
      541 acatgcaaga gatcgttggc ttcctttgag atcgttcggt agtcccccga gagcaacgct
      601 tcagtggttc catcgaaggt tgccgacaga ccatcctgga tatcctccct tgcagcttca
      661 gtgattggcc ccagtgttgc cctgtcgtct tccgcttcgt ctaaggcttc atcaagccaa
      721 cgagtaacaa gcgtttcaat ctcgtatcga gtcatacggt tccctcgtct cgataaagta
      781 aggaatagcc gtctaccctg tgcttcgaac tgcaaggccc tggttgccgc taccgcttta
      841 tccttcgttc caagagatcg ccagacttcc cgcttgccct ggaagtagac acgcagtctc
      901 gtaggaatga ccaaacgagt atagaacggt ttccgtttat tgcgagagaa tgtagccacc
      961 cagcaccctg aggatgtagc cacgatgtag ccataggctt tctctcggga agcgcttgac
     1021 tgattcgccg ctgtttatgc gggtgattga agagtactta agtagaattg gggtggatga
     1081 cgggtttcga acccgcgacc tccggatcca caatccggcg ctctaaccag ctgagctaca
     1141 cccaccacga gggatttgag gaaggttgat attagcaaag ggccgaatgc cgtcgcaacc
     1201 gaatctcgtt acccccgtgc atcgaaagcg gcttgcatct cgaccagaat agcggccacg
     1261 gtcgccgccg gatctactgc atcacgaatg ggtcggccga tcaccagata atcggcaccg
     1321 gaggcgatcg tctgcgtcgg agtcgtcgct ctggcatgat cgtcaatccc tttgccagcc
     1381 ggacgcacac ccggcgtcac gacagcaaat ttcggaccaa ctttctggcg gatagccgcg
     1441 gcttcctcac cggatgccac taccccgtca cagccgacct ctgaagccag caacgccctg
     1501 gctgtgacga ggtcttgcac tgtccgctgt attcccatgt cccgaagatc gttgctgtca
     1561 aagttcgtca gcacggtcac cgccaatagt ttgagcgatg aaccctcttt cccctgcacg
     1621 gcagctgtga gcgccttgcg gttcgcatgg atggttaaga aatccactcc catcgtcgcg
     1681 acgcgtgccg tcgcacgacg aaccgtctct tcgatatcga gaaacttcag gtcgagaaac
     1741 acccgcatgc cccgctctac aatccgctgg accatcgccg ggcctgccgc ggtatagagc
     1801 tcaagaccca cctttacaaa ggagatgtgt ccttgaagtc ggtccaagag ccgatctgct
     1861 tccaccgttg aaggcacatc caatgcaaag atgagtcggt cgcgtgccgc gatcttcacc
     1921 atacccttgc tccttcttca ggccgatcct tgacgatcgg cgacacccta tgataaaaat
     1981 cgacctcttt tgcagaggag ttatctatgc cacgcgttca taaatcgacg attcgacgag
     2041 cccgtcaagc agaccggcgc catgagcgga atcgggcaac tctcaacagt gtgaagacgc
     2101 tcatcaagaa ggttcagtcc gccgtggcgg ggaaaaaggc ggacgaggcc aagaccagct
     2161 tgctggaagc gacttcggcc atcgggaagg ctgtttcgaa aggcgtcctt catcgcaaca
     2221 ccgcatcacg ccgaatttca cgtttggctc tttgcgtcaa tgccctgtcc ggttccggct
     2281 cttaatcgtt cgcacgttcg atacgacccg cgcaggaact cgcttcggtg aggttggagg
     2341 acaaggagcc ggctcggctc gccgatcggc ggtgtcctca cagagcttca acaacacacg
     2401 ctccagtacg attctcggac gactcccgct tccacccttg agcctcccat cagtgtcgag
     2461 aaataggcgc aacgccgctt gaagatgcgg ctcagaaaac cgatcgagga aagagcggac
     2521 cttccatggg tccatgcgca agcctctggc cacctcccct tcacgaccac cattggccag
     2581 cgagtctttg attttccaga gacggcgata ctgccatgca agagaaccga ggatccgaag
     2641 cggggcttct cctgcttcta ggttgcgcgc caagatggaa agggctcgcc cccgacgacc
     2701 ctctgcgatg gcgagggtca aatcaaacac cgaggcgccg ggatccatgc cgcgcagcag
     2761 atgaacatcg tgagcggtga cggaacgatc agacggtata tacgaagcca gcttttccaa
     2821 ttcgcgccgc acgccataca gtgatgaacc acacgactcc tgcagcacct gggatgccgc
     2881 ttcctccaag cgaagcccga cccgttctgc ttcacgggcg atccaagcag gcagctgcgc
     2941 gtcgcggagg ggtgaacaat cgatcacgat tgccgctctg gccagggcct tggaaaattt
     3001 aagccgacca tccagcttcg ggcttac
//
LOCUS       DATMTI010001041         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_846798, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001041 DATMTI010000000
VERSION     DATMTI010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846798"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..449)
                     /locus_tag="VN039_16510"
     CDS             complement(186..449)
                     /locus_tag="VN039_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47600.1"
                     /translation="MNLTSVWKNRRTGKKTSARKPKIRWQLLGLTDPNSVDNTSSMET
                     IRREVTSLARYSFGPDTRLRATAQESKSLGKNHEPVGGRNKRR"
     gene            971..>2212
                     /locus_tag="VN039_16515"
     CDS             971..>2212
                     /locus_tag="VN039_16515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWV47601.1"
                     /translation="MDTSAHVNFHALGLSEALLQDLADAGFAAPTPIQTQAIPPALAG
                     RDVIGCAQTGTGKTAAFVIPIIERLALLPKGQPQALILAPTRELALQTLATIEKLGRS
                     RRISATVIVGGADMQAQVRGLRQRPDIVVATPGRLLDHMWNGTILLSSIKMLVLDEAD
                     RMLDMGFAPQINQIRDALPEQRQTLLFSATLPADLARLIHASVNNPVRVMVAPSATTA
                     EGVTQAVHYTSHADKPDLLLSLLKADQATALVFTRTKHRADRLGRLLGRAGHRVAVLH
                     GDRSLSQRRTALEGFRRGAFRVLVATDIAARGIDVANIGHVINFDLPNCPEDYVHRIG
                     RTARMKTTGRATSFVTGEDQHQLRDIERLLGRAVPVAPGSKASSPAIPAPGGSIRRDG
                     HHSRSQVKRSQIHSSRDARRSH"
BASE COUNT          478 a          579 c          642 g          513 t
ORIGIN      
        1 ctcgtggttc gaaataggta tagacgagga cgctaatcac tcccgtaatg tacagcaagg
       61 ttgccgcagc tagaggattg tgaacgaacc attggatgaa gttctgaaag agggacatag
      121 gtttgctgtt cctattcagc atttaagaga cggcgcggtt tacgcgccgg tatctgccca
      181 gtggcttagc ggcgcttgtt tctccctccg acgggctcgt ggttcttgcc taacgacttg
      241 ctttcctgag ccgtggcacg caagcgggta tccggtccaa agctatacct cgccaacgaa
      301 gtgacttctc gcctgatagt ttccattgag gatgtgttat cgaccgaatt gggatcagtc
      361 agccccagca gttgccagcg gatcttcggt ttacgggcgg acgtcttctt gcctgttctg
      421 cgattcttcc aaactgatgt gagattcatg aagaactcct tttcgtcggg catgaactac
      481 tagccagggc atactgatgg atgaaaccgg gtcagtttcg ataccgacaa cgaattttct
      541 catctgtatg aaagcgggcg ccattcatac caaatgagtg tcaggacagt gaaagctcaa
      601 gctgtctcgc agagaccgga gtgagtaaga ggagtgagcg gagaggacgc agctcaaatg
      661 gtgatggact ctaacatgtg tttcgtgaag gagtcaaatt tctccatcgg ctgatacaaa
      721 ataaggtatt tgggcacaga tagtatgacc ctctcatatc tattgaagaa caggttgacg
      781 tcgggagagc catgctgtat ggtaacactg catttcactc gcggtggtcc ggcgagggaa
      841 tgttgaaact ataacggact tcgcgatcgc atcgcgacca ggtccagttt ggagcggcac
      901 cctgctcggt tgtttctccc ctcggttctc tgatcccgtc atcatgcgat caaactgaaa
      961 ggatatcgta gtggatacgt ctgctcatgt gaatttccat gctctcggtt tgtccgaagc
     1021 actattgcaa gacttggctg atgccgggtt tgccgcaccg actcccattc aaacacaagc
     1081 catcccaccg gcgcttgcgg gccgagatgt catcggatgc gctcagaccg gcacaggaaa
     1141 gacggctgcc ttcgtcattc caatcatcga gcggctcgct ctgcttccga aagggcagcc
     1201 tcaagcattg atcttggcgc cgacgcgcga gctcgcattg cagaccttgg cgacgatcga
     1261 gaaactcgga cggagccgac gcatttccgc aaccgtcatc gtgggaggcg cggacatgca
     1321 agctcaagtg cgaggtttac gacaacgacc ggacatcgtg gttgcgacgc ccggtcgtct
     1381 cctcgatcat atgtggaacg gcacaattct gttgtcgtcc attaagatgt tggtgttgga
     1441 tgaagcagat cggatgttgg acatgggctt tgctccgcaa atcaatcaga tacgcgatgc
     1501 cttgccggaa cagcgacaga cgctgctgtt ttcggccacc ttgccggcgg acttggcgcg
     1561 gctgatccac gccagcgtga ataatccggt gcgcgtgatg gtcgcaccgt ctgctacgac
     1621 ggccgagggt gtcacgcagg cggtgcatta tacatcccac gccgacaagc cggatcttct
     1681 gttgtcgcta ttaaaggccg atcaggctac cgcgctggtt ttcaccagga cgaagcatcg
     1741 tgccgatcgg ttggggcgct tgctgggtcg tgcgggccat cgagtggctg tgctgcacgg
     1801 agatcggagt ctatctcaga ggcgcaccgc gctggaaggt ttcagacgcg gagcattccg
     1861 tgtgttggtg gcgacggata ttgctgcccg agggatcgat gtcgcgaata ttggccacgt
     1921 gattaatttc gatctgccca attgcccgga agattatgtc catcgcatcg gccgcacggc
     1981 tcggatgaag acgaccggcc gcgccacaag ttttgtgacg ggagaagacc aacaccaact
     2041 gcgagatatc gagcgtctgt tggggcgtgc agtcccggtt gctccaggaa gcaaggcttc
     2101 ttcccctgcg attcctgccc cgggcggaag tattcgtcgg gacgggcatc attcgaggag
     2161 tcaggtgaaa cgctcccaaa tccattcctc cagggatgcg agaaggtccc at
//
LOCUS       DATMTI010001042         1784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_590435, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001042 DATMTI010000000
VERSION     DATMTI010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590435"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VN039_16520"
     CDS             complement(<1..727)
                     /locus_tag="VN039_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47602.1"
                     /translation="MMAPRGRLNGPGRRSNEWTDIRARKESADVYKNAGLRPRSLRSI
                     GPDRESAPFDEVKAKRRAPAHLLVWFLFLIVLIPQSSSQPAVTLVLIECILVAFGGVF
                     SLSQILRAGLWSPASLFYIVLALFHLGLIPAWLFGIDPGFTRATDYVWFDGPIGMQSL
                     VITLVAMASYVAGVSAYATLRTIKRDVLAQEGPDAGRQGARRSEYGLAGAVLTTGGVL
                     AWFVIGIAAGGVQIFTASYSTWLN"
     gene            complement(724..1302)
                     /locus_tag="VN039_16525"
     CDS             complement(724..1302)
                     /locus_tag="VN039_16525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWV47603.1"
                     /translation="MALERGRARFHRRHLVLQLLESTFSKLPRRMQRLGVGFLRASDS
                     EFSRAVRYVALRCVSQSCGELIDLKSNCVVLAPERLVLGSRISIHPFCYLDATGTIEI
                     GSDVSIAHSTSILSTNHIWESLGVPIRDQGITLAKTVIEDDVWVGAGVRILAGTTVGT
                     GAIVAAGAVVTKDVPAYAVVGGVPARVLRTRS"
     gene            complement(1292..>1784)
                     /locus_tag="VN039_16530"
     CDS             complement(1292..>1784)
                     /locus_tag="VN039_16530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV47604.1"
                     /translation="LNPSDDRAVAEASSRNRGKRTIIVVGESHSVPSYMRAADVLLVT
                     STREGLPSVVLEALAIGTPVVATRLPGMEWIQSHTVGVELCSLNADDGEWVEAIGRAL
                     QIDRRTIATAFTSSPFIMARASASFRHMWQIPPAADEQVNCDGLIDRTEIDANDGGSE
                     NGT"
BASE COUNT          404 a          546 c          479 g          355 t
ORIGIN      
        1 gattgagcca agtcgaatat gaggctgtaa agatctgcac gccaccggcc gcaataccga
       61 tgacaaacca agcaagcact cctccggtag ttagaaccgc gcccgccaga ccgtactctg
      121 atcggcgtgc gccctgtcgc cccgcatctg ggccttcttg ggccagtacg tcgcgcttga
      181 ttgtcctcaa cgtcgcgtag gcggacacgc ccgcaacgta tgaggccatc gctacaaggg
      241 ttatgactaa ggactgcatc ccgatcggac cgtcgaacca tacgtaatct gtcgcccggg
      301 tgaaccctgg atcgatcccg aatagccagg ccggaataag cccaagatga aatagggcga
      361 gcacaatata gaaaagactt gccggtgacc agaggcctgc tcgcaagatt tgagagagcg
      421 aaaacacgcc gccaaacgcc acgaggatgc actcaatcag caccaatgtc acggccggct
      481 gcgacgacga ctgtgggatg agcacgatca ggaacagaaa ccaaacgagt agatgcgcag
      541 gagctcgacg cttcgccttc acctcatcga atggcgcaga ctcccggtcc gggccgatcg
      601 atcggagaga tcgtggtctt aaacccgcat tcttgtagac gtcggcgctc tcttttcttg
      661 cccgaatgtc cgtccattcg ttggaccgtc gacctggacc attaaggcgt ccacgtgggg
      721 ccatcatgat cgtgtacgca gtacacgcgc cgggaccccg ccgaccacgg cgtatgccgg
      781 aacgtccttc gtcacgacgg cccccgcggc aacgattgcg cctgttccga ccgttgtacc
      841 agccaaaatc ctcacccctg caccaaccca cacgtcgtcc tcgatcacgg tcttcgcgag
      901 cgtgatgcct tggtcacgaa tggggacgcc aaggctctcc cagatgtgat ttgtgctcag
      961 aattgacgtg gaatgagcaa ttgagacgtc gctcccaatc tcgatggtgc cggtggcgtc
     1021 gaggtagcaa aaaggatgga tgctgatgcg tgatcccagc accaggcgct caggtgcgag
     1081 aacgacgcaa ttcgatttta agtcgatcag ttcaccacag ctttgagaaa cgcaacgaag
     1141 cgccacgtac ctcactgccc gcgagaactc cgaatccgat gctcgcaaga atccgacccc
     1201 aagtcgctgc atgcgcctgg gcaacttcga aaacgtgctc tcaagcaatt gaagaacgag
     1261 atgtcgtcga tgaaacctag cccggcccct ttcaagtgcc attctcggaa cctccgtcgt
     1321 tcgcgtcgat ttctgttcga tcgattagcc catcgcaatt gacctgctcg tcggccgctg
     1381 gcggaatctg ccacatgtgt ctgaaagacg cggacgcacg cgccatgatg aacggactgg
     1441 atgtaaaggc ggttgcgatg gttctgcgat cgatctgaag agctcgccca atagcttcga
     1501 cccattcgcc gtcgtctgca ttcagcgagc acaattcaac gccaacggtg tgcgattgga
     1561 tccattccat gccaggcaat cttgtggcca ccaccggagt accaatcgcg agtgcttcca
     1621 ggactacgct cggcaggcct tctctcgtcg acgtgaccaa gagcacgtca gctgctcgca
     1681 tgtaacttgg aacgctgtga gattccccca ctacgattat tgtgcgtttc cctcgattgc
     1741 ggctcgaagc ctccgctact gctcgatcgt ctgatgggtt caag
//
LOCUS       DATMTI010001043         1791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_846860, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001043 DATMTI010000000
VERSION     DATMTI010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846860"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VN039_16535"
     CDS             complement(<1..1182)
                     /locus_tag="VN039_16535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWV47605.1"
                     /translation="MKRVLLVEDNFGDARLAQEMLSESPEFEAHVTCVGRLNQAIEAL
                     TNQSFDVVLLDLSLPDSNKLEGLQRLQPLSKEAPIIVLTGLNDKAQALEALKHGAQDY
                     LFKGTMNSDALIRVIQYSIERRASDARYRSLVANIPGVVYRCQSEKRGALLFASLSFE
                     RLTGYSLDEFMAKASGGYSDLIHPDDRERTMCMVLGAVTRKQPYSIEYRIRRKDRQIR
                     WIFDRGQGVGPACGALPNRDGVLFDITEQHAMEEKFSKADQQLRRAEQFAAMGNLACG
                     VAHDFNNLITAINGFADLVVHDLPVDTRAYNRMLQIRKAGQRAMAMTQHLLALGKTTT
                     KDPAIVDMNLLLTDMERMIQLMIGVRRKVTIVSNPFLGRVRGNPRELEQALLILAMNI
                     AQ"
BASE COUNT          397 a          495 c          460 g          439 t
ORIGIN      
        1 ctgggcgata ttcatcgcca ggatgagcag cgcctgttcc aactctctgg ggttgcccct
       61 gacacgtcct aggaacggat tcgaaacgat cgtgactttg cggcgcactc caatcatcag
      121 ctggatcatg cgttccatgt ccgtcagcag cagattcatg tccacgattg ccgggtcttt
      181 cgtcgtcgtc tttcctaggg cgagcaagtg ctgagtcatc gccatggctc gctggccggc
      241 ttttcgaatc tgcagcatgc ggttgtaggc tcgtgtgtcc acaggcagat catgtacgac
      301 aagatcggca aacccgttga tggcggtaat gagattgttg aagtcatgcg caaccccaca
      361 ggccaggttt cccatcgcgg cgaattgttc cgcccgcctc aactgttgat cggccttact
      421 gaacttctct tccatcgcgt gctgttccgt gatgtcgaac agcacaccgt ctcgattcgg
      481 caatgctccg catgctggtc cgaccccctg cccacggtcg aaaatccaac ggatctggcg
      541 atcttttcga cggattctgt actcgattga atacggttgt ttgcgggtta cggctcccag
      601 caccatgcac atcgtccgtt ctcgatcgtc cggatgaatc aagtctgagt agccacccga
      661 agccttcgcc atgaattcat ctaaggaata tccggtcaac ctctcgaacg aaaggctcgc
      721 aaacaacaga gctccgcgct tctcgctttg acagcgatac accacgccgg ggatgttcgc
      781 gaccagcgat cggtagcgtg cgtcggacgc cctccgttca atggagtatt gaatcactcg
      841 tatcagtgca tccgaattca tggttccctt aaagaggtag tcttgcgcac catgcttgag
      901 cgcctcaaga gcctgtgcct tgtcgttcaa accggtcaga acaataatcg gagcttcttt
      961 tgacaggggt tgcaatcgtt gaagcccttc gagcttattg ctgtccggca acgagagatc
     1021 gagcagcacc acatcgaagg attggtttgt cagcgcttcg atggcctgat tgaggcgacc
     1081 gacacaggtc acatgcgcct cgaattccgg cgattcggag agcatttcct gagcaagcct
     1141 tgcgtcaccg aagttatctt ccacgagcag cactcgtttc atgacggcct cccttgtggt
     1201 tcattcgacg tttcgatgac tcatgcagtg gttgtagagg tatcgaccca actaaacggt
     1261 ttggatgtga cccttgagtc aattctcgtg agccgggctt ccgtctcgct gtcttctacc
     1321 agcaacactt tcgtgagcgc agtcactgaa gaggttggtt caacaatcgt ggccataagc
     1381 ctgtatgcgt ggatcttgct gttccctatg caccacaagc aggacgaaat gaacatagcg
     1441 acaattgagt cgaatgcttc ggagcgattc tctgcgcctt ctcggataaa taccgaaaag
     1501 ctttagccta tttgccgtca caaaggagcc tcccgcgatc tccccgctcg atacttatgt
     1561 attcgtcgaa aaagcctcac agaggagaag attagccaat actaaaagtg ccgcatgggc
     1621 gtttcggtcg gatggtagta agccagattc cgacagtgca gcgacaatga gtccagacgt
     1681 taatgtgcca caacgacgat tatcgatcga aaacggtata gtggctgcat gccagtgact
     1741 ggatcgtctc cctgtctcat atacgagact ctgaatcgga cgagtcatga g
//
LOCUS       DATMTI010001044         1837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_549310, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001044 DATMTI010000000
VERSION     DATMTI010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549310"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VN039_16540"
     CDS             complement(<1..1046)
                     /locus_tag="VN039_16540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV47606.1"
                     /translation="MARFAYLFERFPSFGQTFCYREVAELDRQDIAPPIFSIRNPKGE
                     PPQDWDTRIVERVQCLPDEKELLEAVRRAAKKAKLNPDIIAALDEWGRRSDFLRLYQA
                     IFVGLRLQQMGINHVHAHFAGMAARTAFWINRFFSISFSFTAHANDIFSPRQFEIGLD
                     KLVDKARAIVTETDYAAQFLRERFPHRADRVHRIYNGLDLSEFGHADFSSTPPLVIAV
                     GRLISKKGFADLIRICALLVERGKSFRCEIIGEGSLQNELQRQIGELSLQNVVVLAGA
                     KPQTELRRRLAAANVFVLPSVIDPDGGMDNLPTVIMEAMATGLPVVSTNIGGIPEMVI
                     ENETGFLVQPGNIAA"
     gene            complement(1242..1601)
                     /locus_tag="VN039_16545"
     CDS             complement(1242..1601)
                     /locus_tag="VN039_16545"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWV47607.1"
                     /translation="MIQTIAMPQDTNPNGDIFGGWVTSQMDLGSGILAAKTAQARVVT
                     AAMEGMSFLQPVRVGDTVRCYAHVEKIGRTSMTIPVEVWVTRYMTGEELRVTHGVFTL
                     VAVDESGKPIPVKRGGS"
BASE COUNT          439 a          511 c          472 g          415 t
ORIGIN      
        1 gctgcaatgt tgcctggttg caccaaaaat ccggtctcgt tctcgatcac catttccggg
       61 atgccgccga tgttcgtgga caccacaggc aaccccgtcg ccatcgcttc catgatcact
      121 gtaggcagat tgtccatgcc gccgtcggga tcaatcacac tcggtagcac aaaaacgttg
      181 gccgcggcga gccgtcgcct gagttccgtc tgtggttttg cgcctgcgag cacaacaacg
      241 ttttgtagag ataattcgcc gatctgccgt tgaagctcgt tctgaagcga accttcgccg
      301 atgatttcgc agcggaacga tttcccacgt tccacgagca acgcgcagat gcgaatcaag
      361 tcggcgaatc ccttctttga aattaaacga ccgactgcga tcaccaatgg cggcgtcgat
      421 gagaaatcgg cgtggccaaa ttccgaaaga tcaaggccgt tataaattcg atgaacgcgg
      481 tcggcgcgat ggggaaagcg ttcgcgcaaa aattgcgctg cgtaatctgt ttcagtgaca
      541 atcgcgcgcg ccttatcgac gagtttatcc agtccgattt cgaactgcct cggtgagaaa
      601 atatcgttcg cgtgcgcggt gaaactgaaa ctgatggaaa agaatcgatt gatccagaaa
      661 gcagtgcgcg cagccatgcc ggcgaaatga gcatgcacat ggttgattcc catttgttgc
      721 aatcgcaatc caacaaagat cgcctgataa agccgaagga aatcgctgcg ccgtccccat
      781 tcatcgagcg cagcgataat gtccgggttg agcttcgctt tttttgccgc gcgtcgaacg
      841 gcttccagca gttccttctc gtccgggaga cattgcactc gttcaacgat gcgcgtgtcc
      901 caatcttgtg gtggttcgcc tttcgggttc cgaattgaaa agattggcgg tgcaatgtcc
      961 tgacgatcaa gctctgccac ttcccggtaa caaaacgttt ggccaaacga tgggaaccgc
     1021 tcgaacaggt aagcgaaacg tgccattagc aaaataacga ataacgagtg acgaatgacg
     1081 aacaaatcac gaagcgcgaa cgacgaatgg atacgtcgtc atcccgagcg cagcgaagtg
     1141 gagtcgagag atcccgcggc gaaatggtaa gtttatcatc gggattcccc gacttcgctc
     1201 ggaatgacag gaatacacag gcgcgcgatt gttttcgtcg atcaactccc gccgcgcttc
     1261 actggaatcg gtttccccga ttcgtcgacc gcaaccagcg taaatacacc atgcgtgacc
     1321 cgtagctctt cgccggtcat gtaacgggtc acccaaactt caactggaat ggtcatcgaa
     1381 gtgcgcccga ttttctccac gtgcgcataa cacctgacgg tgtcccccac tctcactggt
     1441 tgcagaaatg acattccttc catagccgca gtgaccacac gcgcttgcgc agtcttcgcg
     1501 gcaagaatcc cgctgcccaa gtccatctgt gaggtgaccc agcccccgaa aatgtcgcca
     1561 ttcgggtttg tgtcctgcgg catggcgata gtctggatca ccagttcacc tttcggtcgc
     1621 tcgctcattt cgccaattta gtccgtcatt tccagtgaag tcgagaaatc ccgatgaaaa
     1681 ggcggatact gttgccacgc gatccttcga cccctgaaag ctttcggggc tcagaatggt
     1741 ccccgactgt tgttccatgt gtttctgttc cagctcgaac gagaagatag aggaagcgga
     1801 agattataaa atcagcatgc aatcgccgta actgtaa
//
LOCUS       DATMTI010001045         1886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_506752, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001045 DATMTI010000000
VERSION     DATMTI010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506752"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VN039_16550"
     CDS             complement(<1..465)
                     /locus_tag="VN039_16550"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HWV47608.1"
                     /translation="MIRINLLPGGPKGRAAKPQYDVRAQALLGIGVILITLVGCWWYS
                     ASLDSELEARQEEKRDKEKQVVQLKEQVKKVQDFEQRKKLLEDKNRIIDQLEQSRMGP
                     VKVLDHVSQSLEPLKVWLTKLGVASDMVELEGKALTNDDVVEFVNNLRRTDYF"
     gene            complement(462..1568)
                     /gene="pilM"
                     /locus_tag="VN039_16555"
     CDS             complement(462..1568)
                     /gene="pilM"
                     /locus_tag="VN039_16555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HWV47609.1"
                     /translation="MLISLKKLVETDIIAMLTPRRQLVGLDIGSSAIKVAQLKESKGR
                     YFLQKFGVKPLEPEVIVDGTVMDEGRVISAIGELFEQANVKNKHVAISISGHAVIVKK
                     INLPPMPDEELEGQVKLAAEQYIPFDINEVNIDFHVLPSDESEDQHGDMSVILVAAKK
                     DKINELTELVKAAGLMPMVMDVDAFAVENMHAINYPMAQEETTALVNLGASVMNVNII
                     RAGSSLFTRDIPLGGNRYTEAIQREIGLSFEEAEESKKNDGRGDSGGVSLTGVMDSVN
                     AEVASEIARTVDYFKTSTANAELSRVLICGGVARAKGLIQQLGDRMQLPVEMADPFAE
                     IDITGCDIAPDLLVDLAPSAAVGVGLALRAVGDR"
     gene            complement(1584..1796)
                     /locus_tag="VN039_16560"
     CDS             complement(1584..1796)
                     /locus_tag="VN039_16560"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWV47610.1"
                     /translation="MKHPKYALSEAGFKAYQVCNLFDISKATLFRWEREGVITQPARD
                     WRNWRLYTRKNVDEIEKVIRARKVVA"
BASE COUNT          434 a          567 c          398 g          487 t
ORIGIN      
        1 gaaataatcc gtacgccgca agttattcac aaattccacc acatcgtcgt ttgttagggc
       61 cttcccttcc aactcaacca tatcggatgc cacacctagt tttgtgagcc atactttgag
      121 cggctctaaa ctctgactaa catgatcgag aaccttcacc ggacccattc tggattgctc
      181 gagctgatct ataatccggt tcttgtcctc aagaagcttt ttgcgttgtt caaaatcctg
      241 tactttcttg acttgttctt tcagctgaac aacctgtttt tctttatctc gcttttcctc
      301 ctgcctagct tcaagctcgc tgtccaatga agccgaatac caccagcatc cgaccaatgt
      361 aatcaggatc acgccgatgc cgagcagcgc ctgtgcgcgc acgtcatact gaggtttggc
      421 cgctcttcct ttgggcccac cggggaggag gttgattcga atcatctgtc ccccaccgct
      481 ctcaacgcca atccgacacc gacggctgcc gacggagcca aatccaccag taggtcaggg
      541 gcgatgtcac accctgtaat atcgatttcg gcgaagggat cagccatttc tacaggcagt
      601 tgcatcctat cgccaagctg ttgaatcagc ccttttgcgc gggctacgcc accacagatg
      661 agaacacgac tgagctcagc attcgcggtc gaggtcttga agtagtccac cgtccgagca
      721 atctcggacg cgacttccgc attcacactg tccataacgc cggtaagcga caccccgccg
      781 gaatcgcctc ttccatcgtt tttcttactc tcttcggctt cctcaaagga gagccctatc
      841 tcgcgttgaa tcgcctcggt gtacctattc ccccccaacg gaatatcacg cgtaaacaat
      901 gacgaaccgg cacggatgat gttcacattc atcacgctcg cgccgagatt caccagcgcc
      961 gtcgtttctt cctgcgccat cggataatta atcgcgtgca tattctcaac cgcgaacgca
     1021 tcgacatcca tgaccattgg catgagtccg gcagccttga cgagctcagt caattcatta
     1081 atcttgtctt tcttagcggc aacaagaatc accgacatat ccccgtgctg atcttcagac
     1141 tcatccgaag gcaagacatg aaagtcgatg ttcacttcgt tgatatcgaa gggaatgtac
     1201 tgctccgctg ccaatttcac ctgtccttcc aactcttcgt ccggcatggg cggcagattg
     1261 attttcttca caatgacagc gtgccccgaa atcgagatgg cgacgtgctt attcttgacg
     1321 ttggcttgct caaacaattc tccaatagcc gaaatcacac gcccttcatc catgaccgtc
     1381 ccatccacaa tcacttccgg ctcaagcggt ttgacgccga acttttggag aaagtaccgt
     1441 cccttacttt ctttgagctg agcaacctta atcgcgctcg atccgatatc aagtcccact
     1501 aactgccggc ggggcgtcag catcgcaatg atatcggtct ccacgagctt tttgagtgag
     1561 atcaacatgc gttacctgcc actttatgcg acaacctttc gggcgcgaat aactttctcg
     1621 atttcatcca cgttctttct cgtatagagc cgccaatttc tccaatcccg agcgggttgg
     1681 gtaatgacac cctcccgttc ccaacgaaag agcgtcgcct tggaaatgtc gaacaagtta
     1741 catacctgat aggccttgaa gcccgcttca ctcagcgcat atttaggatg tttcactgta
     1801 gtatttacac tgctctgacg ccttcggata ctcacaaggc taagtatagt caatatgctc
     1861 tacctgtcaa ggaagcgacc gaagtc
//
LOCUS       DATMTI010001046         5899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_464301, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001046 DATMTI010000000
VERSION     DATMTI010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464301"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VN039_16565"
     CDS             complement(<1..237)
                     /locus_tag="VN039_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47611.1"
                     /translation="MSRLLVCPPDFFGIEYEINPWMRLTNRVDHGRAVRQWHELMRVL
                     EDDLGVVLERMTPIVGLPDLVFTANAGIVAGRTAV"
     gene            complement(234..1478)
                     /locus_tag="VN039_16570"
     CDS             complement(234..1478)
                     /locus_tag="VN039_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HWV47612.1"
                     /translation="MNHYQESVYLQGHIIDSLVLAKVLDLILMMGGTFDLEDVHIGKT
                     RAEPSHARIRIRTASRALLDDILRKIQPHGASIEREADCRTDRAPADGVLPDDFYATT
                     HLPTQIRLNDRWLDVDRIEMDLAIAVNEAGSVAQAVPMGEVRQGDPIVVGREGVRVIP
                     LQRPQERDVFGFMESQVSAERPHGHIIADIATRMRQLRERHRQGQADSHVLLSGGPAI
                     IHAGGREALTWLIEQGFVHILFCGNALAAHDMEADLYGTSLGYGLTAGRAVPHGHEHH
                     LRTINRIRSIGSIEAAVTSGAIKQGIMAACIRQGVPVVISGTIRDDGPLPGVITDSVL
                     AQRAMRALIPGVGLALLVASTLHSVATGNLLPATVPTVCVDVNPAVPTKLADRGSFQA
                     VGLVMDAASFLSELARLLGRST"
     gene            complement(1545..2591)
                     /locus_tag="VN039_16575"
     CDS             complement(1545..2591)
                     /locus_tag="VN039_16575"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HWV47613.1"
                     /translation="MTLPIDPFLTGNLPGIGGEIRTVPEDFQVDERPLYLPCGEGEHL
                     YVTITKRGLSTPDLVRRLSSTLCVKAQAIGVAGLKDARAVTTQMVSLQGIRPEQISRL
                     KIDDTILSVQILGRHRNRLRTGHHSGNRFCLVIRHVADHAAETVPAVLQQLSTRGVPN
                     YFGPQRQGKDGENYRIGALLLHDARRREKMSRATRIWYLNSYQSFLFNRILARRIDHL
                     DRVFVGDWAMKSDNGACFQVEDAEKEQPRADRFEISPTGILFGSRVSWASGEPGQIED
                     AVIAEAGATKEGLVAAAKACGFRGERRALRIPLAELEWSLRDDALTLSFSLPAGAYAT
                     SVLRELMKVSPTKP"
     gene            2830..3549
                     /locus_tag="VN039_16580"
     CDS             2830..3549
                     /locus_tag="VN039_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWV47614.1"
                     /translation="MSDHDVSVLTQQHPPLAGCRAVHLTDLHLDRYYPRHDRIVESVK
                     ELHPDWIFITGDLLNVPEGLPHLFRFLDQLRAIAPLYMTLGNHDHYSGVPVSQFSELA
                     DQHKITLLVNQSTVVSTGRGELAIVGVDDPSLHRADLGCVPPRVDSRFTLLLAHAPNI
                     LDYVEPHHAIDLILCGHSHGGQWRVPAIPTFWLPPGCNGRVAGWHESGRHKLYVNRGL
                     GWSFLPFRLNCRPEVAVIEWI"
     gene            4159..4392
                     /locus_tag="VN039_16585"
     CDS             4159..4392
                     /locus_tag="VN039_16585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HWV47615.1"
                     /translation="MTTCYFCKGRVSTQLTSVDFRWGEDLKIIEQVPAEVCEQCGETY
                     FQSQVYKDLERLVQHPSRVIQHRSVDVLQYSAA"
     gene            complement(4449..5084)
                     /locus_tag="VN039_16590"
     CDS             complement(4449..5084)
                     /locus_tag="VN039_16590"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV47616.1"
                     /translation="MSKRHEQGQINPALLVVLIVLTVTAVWVWKRLSVDAQDYIVDQA
                     VPMAATGVALAVLLFIPIRAFRRRSTRTRERTRLLTLFEHESAWDKRLELAFALLELN
                     GYRIAGLESAVPALKELFATTLQRALDDKQHHIRGMAASHLGALQDMSVVPLLVKALE
                     DDHAYVRSCAALGLGRLRATETRDRLKTVMEQDWDQTVRSRAREALERMRE"
     gene            5237..>5899
                     /locus_tag="VN039_16595"
     CDS             5237..>5899
                     /locus_tag="VN039_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWV47617.1"
                     /translation="MENHPQNSAIRRVMVGTDRSKTADQAVLWAAQFADRYGAELFVV
                     QVILPQHPSSTEFGAAEHTRAAAAGDELGNYVRQIAGERGHSLAVIDDDPASAILRAA
                     EREDVDVLVVGNSGMAGRKQFLLGNVPNRISHNSRCTVVIVNTQSAAKEPGAEFVHAF
                     RPQGESQVSQPRLGTRAAHIATVVAKHGLKELLGQPEASDISIRRQQAKRLRAALEEL
                     GPT"
BASE COUNT         1266 a         1670 c         1655 g         1308 t
ORIGIN      
        1 gacagcggtc cgtccagcca cgatgccggc attggccgta aagacgagat cgggcaaccc
       61 aacaatggga gtcattcgtt cgagcacgac gccaagatca tcctcaagca cacgcattaa
      121 ttcatgccac tgtcgcacgg cccgtccgtg atccacacga ttggtaagcc gcatccaggg
      181 attgatctca tactcaatcc cgaaaaaatc cggaggacag acgaggaggc ggctcatgtc
      241 gatctcccca acagcctggc gagttccgac agaaacgatg cggcatccat gaccaggccg
      301 accgcctgaa agctcccgcg atcggccagc ttggtcggga ccgccgggtt cacgtccaca
      361 cagaccgtgg gcacggttgc cggcagaaga ttccccgtcg ccacggaatg gagcgtcgag
      421 gcgacgagga gagccagccc aacgccgggg atcaaggcgc gcatcgcgcg ttgtgccaga
      481 acggaatccg taatcactcc gggcaaggga ccatcatcac gaatcgttcc ggatatgacc
      541 accggcaccc cttgccggat acaagccgcc atgattccct gtttgatggc ccctgaagta
      601 acggccgctt caatactgcc gatcgaacga atccggttaa tagttctcaa atggtgttca
      661 tgcccatgcg ggactgcgcg tcctgctgtg agcccatagc ccagcgacgt tccatacaga
      721 tccgcctcca tatcatgggc ggcgagggca ttgccgcaga acaatatgtg cacaaacccc
      781 tgttcgatca gccaggtcag ggcctctcgt cctccggcat gaatgatggc cggtccaccg
      841 gacagcaaga cgtgcgaatc tgcctgcccc tgcctgtgcc gctccctcaa ctgccgcatt
      901 cgagttgcga tgtcggcgat gatatgacca tggggccgtt cagccgagac ctgggattcc
      961 ataaacccaa atacatctcg ctcttgcgga cgctgcagcg gtatgacacg caccccctct
     1021 cgaccgacca cgatcgggtc cccttgacga acttcaccca tcggcacggc ctgggcgacg
     1081 gaacccgcct cgttgaccgc gatcgcgagg tccatctcga ttcgatccac gtcgagccat
     1141 cgatcgttga gccggatctg ggtgggcaaa tgggtcgtgg cgtagaaatc atcgggtaat
     1201 actccgtcgg cgggtgccct atcggtccgg caatctgcct cacgttcgat cgaggcaccg
     1261 tgaggttgaa tcttcctcag aatgtcgtct aagagcgctc tcgaggcggt tcggattcga
     1321 atacgggcat gagacggttc cgccctggtc ttgccgatgt gaacatcctc caggtcgaag
     1381 gttcccccca tcatcaggat gagatcgagc actttcgcga gcacgagaga gtcgatgatg
     1441 tgcccttgaa ggtacacgct ttcctggtag tggttcatcg cggtactcac tgtatatgac
     1501 atattttacc accggcggcg gctggaggaa gaaaatgaga ggaactaagg cttcgtggga
     1561 gatactttca tcagctccct gagtacgctc gtagcatagg cgccggcggg taaactgaac
     1621 gagagggtga gggcatcgtc cctaagagac cattcaagtt cagcgagagg gatgcgaagc
     1681 gctctacgct cgccacggaa tccacaagcc ttcgcggctg cgacgaggcc ttccttcgtt
     1741 gcgcctgctt cggcgatgac ggcgtcctcg atttgaccgg gttcgccgct tgcccaggat
     1801 acccgcgaac caaagaggat gcctgttggg ctgatttcga aacgatccgc tcgcggctgt
     1861 tccttctcgg catcctcgac ttgaaagcag gcgccattgt ccgacttcat ggcccaatca
     1921 ccgacgaaga ctcgatcgag atggtcgatc cgtcgcgcaa gtatccgatt gaataagaat
     1981 gattgataag aattgagata ccagatgcgg gtggcccgac tcatcttctc acgccgccgt
     2041 gcatcgtgca gcaatagagc gccgatgcga tagttctcac cgtctttccc ttgccgctga
     2101 gggccgaagt agttgggcac gccgcgtgtg ctcagttgtt ggaggacggc cggtacggtt
     2161 tcagccgcgt gatcggcgac atggcggatg actaggcaga agcgattgcc ggaatgatgg
     2221 ccggttcgta gccgattacg atggcgcccg aggatttgca cgctgaggat agtgtcatcg
     2281 attttgaggc gagagatttg ttccgggcgg attccttgca aggacaccat ctgagtcgtg
     2341 acggctcgtg cgtccttcag gcccgccacg ccgatggcct gtgctttcac acacagggta
     2401 gaagagagtc gacggaccag atccggcgtg gaaagaccac gtttagtgat ggtcacgtac
     2461 aaatgttcac cttccccgca aggaagatag agcggccgtt cgtcaacttg aaagtcttcg
     2521 ggcacggtgc ggatctctcc accgattcca ggcaagttgc cggtgaggaa ggggtcgatt
     2581 ggtagcgtca cacgttttcc ttcattggaa tgacggctgt ccgagaatat gtgtcgtcaa
     2641 acctgatgag aaacaagccc atggtatact tttctcgtcc atgcggaaac gatcaggcaa
     2701 acggcaggtc gcggaatgag caggcgacgg acggtgtcat ggcctgatcg catgcgatcg
     2761 tgtatcggct cttgcttaag cgagccactc tatcggacgt tcagcctggt tccttattgg
     2821 gaatggggtt tgtccgatca tgacgtttcc gtcctcactc aacaacatcc gccccttgcc
     2881 ggttgccgcg cagtccatct cacggatctg cacctggacc gatattatcc aaggcatgat
     2941 cgtatcgtcg aatccgtgaa ggaacttcac cctgattgga ttttcatcac cggggacctg
     3001 ctcaacgtac cggaaggtct gccccacctc tttcgattcc tcgaccagtt acgcgccatc
     3061 gcgcctctgt acatgacatt gggcaatcac gaccattata gcggagtgcc tgtgtcacag
     3121 ttttctgaac ttgccgatca gcacaagatc acgttgctgg tgaaccaaag taccgtcgtt
     3181 tcaacgggca ggggagagct ggcgatcgtc ggcgtcgatg atccatcgct tcatcgcgcg
     3241 gaccttggat gtgtcccgcc tcgagtagac tctcgtttta cgttgctgtt ggcacacgct
     3301 cccaacatcc tggattatgt cgagccgcat catgccattg acttgattct ctgtggacac
     3361 agtcatggcg gacagtggag ggtaccggca atccccacct tctggcttcc tcctggctgc
     3421 aatggccgcg tggcggggtg gcacgaatcc ggccggcaca agttgtacgt caaccgaggg
     3481 ttgggttggt ccttcctccc atttcgtttg aactgccggc ctgaggttgc cgtgattgag
     3541 tggatataag aataggccaa gccgcaaccc atgatcgtca ctctcgcatc cgttcgaacg
     3601 cttctctggc cctgcttcta acggttgatc ccaatcctgt tccatgacgg ttttcagtcg
     3661 gtcgcgtgtc tcctttgctc gcagtcggcc aagccccaag gctgcgcagg aacgcacgta
     3721 ggcatggtca tcctccagta ccttgacgag ccacccttcg tcttttacgg aggcaagatt
     3781 acatcacccg aagttggata actggatttc tcttgttctg tacgatagac tcatcgcgaa
     3841 tgaatcttga ggaaattcgg cgaaaggtac agttacgacg gtaccgtatt tcatggacac
     3901 atacagagaa gctcaggcgt cgacaaatca gtagcgacat gatcgaacgt gcaatcgaga
     3961 ccggcgttct gattgatcgt atcctaatga tccaagagga ccctcttgtt tactcttggg
     4021 atattcctcg gaagaccgtc cgcttcatgt ggtctgcggc aaattcgatg acgatgagct
     4081 cttgattatt accgcctatg aaccctctgc caccgagtgg aagtccgatt ggcgaacacg
     4141 gaaaggaggc gacgagctat gacaacgtgt tacttctgta aaggcagagt gagcacgcag
     4201 ttgacgagcg tggatttccg atggggcgag gacctcaaga tcattgaaca agtgccggca
     4261 gaagtctgtg agcaatgtgg tgagacatac ttccagtcgc aggtgtataa agatctggag
     4321 cggttagtgc aacatccatc cagggtcatt caacatcgtt ctgtcgatgt gctccagtat
     4381 tcagcagcct gatttctgag gccattgtga cggttccacc tcatctccat ccatgaagcc
     4441 tctggcttct actcccgcat ccgttccagt gcttccctgg ccctgcttct gacggtttgg
     4501 tcccaatcct gttccatgac ggtcttcagc cggtcacgag tttccgttgc ccgcagtcgc
     4561 cccaagccca aggctgcgca ggaacgtacg taggcatggt catcttccag cgccttgacc
     4621 aatagcggga ccaccgacat gtcttgtaac gcgcccagat gactggcggc cattcctctg
     4681 atgtgatgct gtttgtcgtc cagcgctcgt tgtaaggtcg tggcaaaaag ctccttcaag
     4741 gcagggacgg ctgattcgag cccggcgatt cggtacccgt tgagttcaag caaggcaaaa
     4801 gcaagctcaa gccttttatc ccaggcggat tcgtgctcga acagggtcaa gagcctggtt
     4861 cgttcccgcg ttctcgtact gcggcgacgg aacgctctta taggaatgaa cagtaggaca
     4921 gcaagcgcca cgccagttgc tgccattggt actgcctggt cgacgatgta gtcctgtgcg
     4981 tcaaccgaca gccgtttcca cacccatacc gcggtcacgg taagcacgat cagaacgaca
     5041 agcaaggcag ggttgatctg cccttgttcg tgacgcttcg acataggtga aggatcatac
     5101 ggacgtgtcg attggccgtc aacgaatcga cgcgctccac gttctgagct tctcttccgt
     5161 cctcatataa ttgacatgca attgcggcaa tagtcatact cttttgcgat catcgtcagt
     5221 aaggtaggca aggattatgg agaatcatcc tcagaatagc gccatacgac gcgtgatggt
     5281 gggcaccgat cgttcaaaaa cggctgatca ggctgttctc tgggccgctc aattcgctga
     5341 ccgctacggt gcagaactgt ttgtggtgca ggtcatcttg ccgcaacatc cgtccagcac
     5401 agaattcggc gcagccgagc acactcgggc tgccgcagca ggcgacgagc tggggaatta
     5461 tgtaagacag atcgccgggg agcgcggaca ttcccttgcc gtcattgatg atgatccggc
     5521 ctcggcaatc ctacgcgccg ctgagcggga agatgtcgat gtcttggttg tgggtaattc
     5581 cggaatggcg ggacgaaagc agtttttact gggcaatgtc cccaatcgta tcagccacaa
     5641 ttcccgctgt acggtggtga tcgtgaatac tcagtcggcc gcaaaggagc ctggcgcgga
     5701 atttgtacat gcttttcgac cacagggtga atcccaagtc tctcaaccgc gtctggggac
     5761 tagagccgcg cacatcgcta cggtggtggc aaagcatggt ctgaaagagc tgcttggcca
     5821 accagaggcg tcggatattt ccattcgacg tcagcaagcc aagcgcctac gggctgcgtt
     5881 ggaggaactg gggccgacc
//
LOCUS       DATMTI010001047         1559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_633231, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001047 DATMTI010000000
VERSION     DATMTI010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633231"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..837)
                     /locus_tag="VN039_16600"
     CDS             complement(55..837)
                     /locus_tag="VN039_16600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWV47618.1"
                     /translation="MKFDLTVAVREEAGKGAARSMRRAGKIPAVLYGQGECLLLTANP
                     EELVKILKSHAGSTALISLTVNGAQSKQNRTALLRDYQIDPVTGAVLHADLFEISMSK
                     PIRVKVPLKVIGGIPAGVKEGGVLHHNMRDMHVECLPAALPDHIEVDASALTIGSGIH
                     VKEIGAREGVRFLDDPDQMVVSVAAPMSDAKLEALLTSGAGAAGEPEVVAKGKEVGAE
                     GAAGAEPAKAGAAAPAGEAKAGEKKEAAAAPKAEKKEAEKKK"
     gene            complement(889..>1559)
                     /locus_tag="VN039_16605"
     CDS             complement(889..>1559)
                     /locus_tag="VN039_16605"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWV47619.1"
                     /translation="YFGYARQDRKDQPRVPITAKLVADLITTAGADRVLSMDLHAGQI
                     QGFFNVPVDHLYALPVLLEYIVKKKISDLVVVSPDAGGVERARAFAKRLQANLAIIDK
                     RREGPNQAQIMNIIGDVQGKSVLLLDDMIDTAGTIVQGAQACLDQGARDVITACTHAV
                     LSGPALERLQASCLSQVVVTNTIPLRGKELACPKLHQLSVAPLLGEAIRRIHEDESVS
                     SLFA"
BASE COUNT          311 a          483 c          398 g          367 t
ORIGIN      
        1 gagtctgggc ataggttttt ccgggattgc ccagtccaac gagcaggtgc aacgttactt
       61 cttcttttca gcttctttct tttcagcctt tggagcggct gcagcttctt tcttttcgcc
      121 ggccttcgct tcacccgcag gcgctgcagc accggctttg gcgggctcgg cacctgctgc
      181 gccttcagcc ccaacctcct tgccttttgc cacgacttct ggctcacccg ccgcccctgc
      241 accgctggtg agcagcgctt cgagtttggc atccgacatt ggcgcggcaa cactgactac
      301 catctggtca ggatcatcca aaaaacggac accttcgcgt gctccgattt ctttcacatg
      361 aatgccgctg ccgatggtca aagccgacgc atcgacttct atatgatcag gcagagcggc
      421 cggaagacat tcgacgtgca tatcgcgcat gttgtgatgc agcacgccgc cctccttgac
      481 accggcggga atcccgccga tgactttgag cggaaccttc acccgaatcg gcttgctcat
      541 ggaaatctca aagaggtccg cgtgcaggac cgccccggtc accggatcga tttgatagtc
      601 gcgtaagagg gctgtgcgat tctgcttgga ctgtgctccg ttcaccgtga gcgaaatcag
      661 cgcggtgctg cccgcatgag acttgaggat cttgactaat tcctcaggat tggcagtcag
      721 caacagacat tccccttggc cgtagagcac ggccggtatt tttcccgccc gccgcataga
      781 tcgcgcggct cctttccccg cttcttctct gactgccact gttaaatcga atttcatgat
      841 cctcctccgt ctcctctgct tcttacgcag cacccagtcg ataatggctt aggcgaacag
      901 tgaactcacc gactcatctt catggatccg cctgatggct tcgcccagca gaggcgccac
      961 cgacaattga tgcaacttcg gacaggccaa ctctttccct cgcaacggaa tcgtgttggt
     1021 caccaccact tgggagagac acgacgcctg taaccgttcc agagccggcc ccgacagcac
     1081 cgcatgggta catgccgtaa tcacgtctcg agcgccttga tcaagacagg cctgcgcccc
     1141 ttggacaatg gtgcccgccg tatcgatcat gtcatcgagg agcaacacgc tttttccctg
     1201 cacgtccccg atgatgttca taatttgcgc ttggttcggc ccctcgcggc gtttgtcgat
     1261 gatggccaga ttggcttgaa ggcgttttgc aaatgctcgg gctcgttcga ctccacctgc
     1321 atcgggcgag acgacaacta gatccgatat ttttttctta acaatgtact ccaacaacac
     1381 cgggagtgca tacaagtgat cgaccggcac gttgaaaaat ccctgaatct gcccggcgtg
     1441 aagatccatc gacaacacac gatcagctcc ggcagtggtg atgagatcgg cgaccaattt
     1501 ggcagtaatg gggacgcgcg gttgatcttt acgatcctga cgggcatacc cgaaatagg
//
LOCUS       DATMTI010001048         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_760763, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001048 DATMTI010000000
VERSION     DATMTI010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760763"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /gene="mazG"
                     /locus_tag="VN039_16610"
     CDS             <1..834
                     /gene="mazG"
                     /locus_tag="VN039_16610"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HWV47620.1"
                     /translation="NGCPWDRKQTHESLKPYLLEEAYEVIETIDQRDEQKLQEELGDV
                     LLQVLFHSQIAAETGSFTVEGVIETLATKLIRRHPHVFDNGDQSRNASTSEQVLSQWE
                     EIKRAEREAAGTKQSALDGVPKILPALLRAYQIQARAARVGFDWPQSEAGLEQILAKV
                     AEEIGELREALASQRTRMESEPNRTHTNEEIENELGDLLFSLVNLARFLKANPEDGLR
                     RATNRFIDRFHLVEAQAAEKGRSLRDMTLAEMDQLWDEAKRRLRRPQPDITPHVGDRV
                     P"
     gene            831..1448
                     /locus_tag="VN039_16615"
     CDS             831..1448
                     /locus_tag="VN039_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47621.1"
                     /translation="MTKNQEPYEEGKRAGAHPLLVVFGILVGLWLFVALIVPSSKNKQ
                     ATGTEGPNVSVVEDPEAAPVMFKVQTTILDMNTLGLVVPSQATDSQIVALLKRLKQAR
                     LDDTLGEQLPSTTPGHKLGNHAIADIFIFSDRHYAEVDTIRTLAQGAHAPGTLYPNAV
                     PFEVAMEHVRGHYRIDLNDTGSPDRGSLGFADESGVHSKHYRRIF"
     gene            complement(1482..1898)
                     /locus_tag="VN039_16620"
     CDS             complement(1482..1898)
                     /locus_tag="VN039_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47622.1"
                     /translation="MPLAENTFSIVSVNGPFREPREQVFSYDYSIQRSTWVTPHGVRV
                     KVSIPDELEVLKRRLFGPVVGSPGQQLMMSNILSKAIAGWKMRVAEGESMLTERRDVM
                     VSPFIGPLAHLFPKLEALFEADQSAVREEVRRRVGI"
     gene            2161..3009
                     /locus_tag="VN039_16625"
     CDS             2161..3009
                     /locus_tag="VN039_16625"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV47623.1"
                     /translation="MAKKGYPGLVLVPPSDRTILRRRLLRWALLIIALIWCASTLPLG
                     PPVPDDRENCQPGVGQEGSNSPAENCGQLQRVTEPPPNQSEFSLTPVSQAEGLGESRN
                     PPPSPTPDPGRSATQNDRDRDSQPKSPDPIDHPSGHDVAPLGNEKSTSSLPTAKSVQN
                     PDADVRLAEQGDAFAQYRLGRYYARRDGPRTPESVSWYKKASHGLHRLAGTGNGQAMY
                     VLGVMYAYGRGVTRDTEQARHWLTQAIEYKITAAQPVLANLAVQPHTTPNSKAAEQAK
                     NLKQQN"
     gene            3072..>3793
                     /locus_tag="VN039_16630"
     CDS             3072..>3793
                     /locus_tag="VN039_16630"
                     /EC_number="1.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004128 - cytochrome-b5 reductase
                     activity, acting on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-cytochrome b5 reductase"
                     /protein_id="HWV47624.1"
                     /translation="MNGIARIKTKTPSPYKLTHIEVDTHDTKTFRFELPANATLDMLP
                     GDFLYVHATINGKHVKRPYTPSSLVGTTGFFDLTVKRYAAGSVSRYLHDQRIGDTVLM
                     SGPNTGGHWVDGMAKRAGFVAGGTGITPMISIIRWILTSKITAELFLIFANKTESDVI
                     FRREWECNVQNYPNFHCHHVLEQPPPGWEGGIGRVTPEILRHHLPAPGSDTCVFLCGP
                     PPMADSLEAVLKELGYPQQAIIL"
BASE COUNT          899 a         1112 c         1030 g          752 t
ORIGIN      
        1 aatggttgtc cttgggaccg aaaacagacg cacgagtcac tgaaacctta ccttctcgaa
       61 gaggcgtacg aagtcatcga gaccatcgat caacgtgacg aacagaaact tcaggaagaa
      121 ctcggggacg tgctcctaca agtcctcttc cacagccaga ttgccgctga gaccggctca
      181 tttacggttg agggcgtgat cgagacgctc gccacgaaac tgatccgcag acatccccat
      241 gtattcgaca acggtgacca atcaaggaat gcctcgacca gcgaacaggt cttgagtcaa
      301 tgggaggaga tcaagcgtgc cgaacgggaa gctgcgggga cgaagcagtc cgcactcgat
      361 ggagtgccga agatcttgcc ggcattgctg cgggcgtatc aaattcaggc gagggcagcc
      421 cgagtcggct tcgattggcc acagagcgag gctggcctgg aacagatcct cgcaaaggtg
      481 gctgaagaaa tcggtgaact gcgcgaggcg ctggcgtcac aacggacaag gatggaatcc
      541 gagcccaatc ggacccatac caatgaggag attgagaacg agttgggtga tctgctgttc
      601 tctctcgtca acctcgcccg ctttctcaag gcaaatcctg aagatgggct gcgtcgagcc
      661 acgaaccgtt tcatcgatcg ctttcacctg gtggaggcac aagccgcaga gaagggccgg
      721 tcgttaagag acatgacgct ggccgaaatg gaccagctat gggacgaagc caaacgacgg
      781 ttacggagac cacagccgga catcacaccg cacgtcggag atcgcgttcc atgacgaaga
      841 accaagagcc ctacgaagag ggcaaacgtg cgggggccca tccgctgtta gtggtatttg
      901 gcattctcgt cggactatgg ttgttcgtcg ccctgattgt ccccagttcg aagaacaaac
      961 aggcaaccgg tactgaaggt cccaatgtct cggtcgtcga ggatccggaa gccgcgcctg
     1021 tcatgtttaa ggtccagacg accattctgg atatgaacac cctggggctg gtcgttcctt
     1081 ctcaagcgac ggatagtcag atcgtcgcac tcttgaagcg cctcaaacaa gcacgcctcg
     1141 acgatacact cggtgaacag ctcccctcga ccacgcctgg ccacaaactc ggcaatcacg
     1201 ccattgccga cattttcatc ttttcagaca ggcactacgc ggaagtggat acgatccgta
     1261 cactggcaca aggagcccat gcgccgggga cgctttaccc gaatgctgtt ccattcgagg
     1321 tggccatgga gcacgttcgc ggccactacc gcatcgactt gaatgatacc ggcagtccgg
     1381 acaggggatc gcttggtttc gcggacgaat caggtgtcca ctcgaagcac taccgaagaa
     1441 tcttttgacg gatccggctc gcgcgtcttt cccgctgaga gtcagatgcc tacacgtctc
     1501 ctcacttctt ctcggaccgc cgactgatcc gcttcaaaca acgcctctag cttcgggaac
     1561 agatgggcca acggtccgat aaacggcgac accatcacat cacgtcgttc cgtcagcata
     1621 ctctcccctt cagccacccg catcttccag ccggcaatcg ctttggagag aatgttgctc
     1681 atcatcaatt gctggcccgg cgagccgacc accggcccga agagacgacg tttaagcacc
     1741 tccagctcat ccggtatgga aaccttcacg cgcacgccat ggggcgtcac ccaggtggac
     1801 cgttgaatcg aatagtcata ggaaaagacc tgctctcgcg gctcacgaaa tgggccgttg
     1861 acgctgacga ttgaaaaggt gttttcagct aatggcatgg tttgctcgct ttcctgggtg
     1921 acaatggtgc cctactagaa tcgacccact caacctacta taagagcttg aagttggggg
     1981 ggacaagagg atatttatac tggtagcgca actgatataa gacggatggg gcgaacgttt
     2041 gcttagtatt cagaactccg cagttaccgc caatgctaca gcggcggcgg ggttccggag
     2101 cacgagacag cattttgaca cactgatata ctccgaccaa gggttccttc gggagaaggc
     2161 atggccaaga aaggctatcc tgggctcgtc ttggtgccgc ccagcgatcg aacgatcctg
     2221 aggcgccgac tgcttcgttg ggctttgttg atcatcgcgc tgatctggtg tgcatcgaca
     2281 ctccctctgg ggcctcctgt ccccgacgac agggagaact gtcaacccgg tgtcgggcag
     2341 gaaggctcga acagtcccgc tgaaaactgc ggccaactgc aacgggtcac ggagccgcca
     2401 cccaatcaaa gtgagttcag ccttacgccg gtttcacagg ctgaaggcct gggcgaatcc
     2461 aggaatcccc cgccttcgcc tacccctgat cccggtcggt ccgcgaccca gaacgatcgt
     2521 gaccgggact cccagccgaa atcccccgat ccgatcgacc acccgtctgg tcacgatgtg
     2581 gcaccattag gcaacgagaa atcaacatca agtctgccga ccgcaaaatc tgtgcaaaat
     2641 ccagatgccg acgttcgcct tgccgaacaa ggcgatgcat ttgcccagta tcgcctcgga
     2701 cgttattacg cacggcgtga cggaccacgg acgccggagt ccgtcagttg gtacaagaaa
     2761 gcttctcatg gtctgcaccg cctggctggg accggtaatg gacaggcaat gtacgtcctc
     2821 ggggtcatgt atgcctatgg gcgcggagtg accagggata cggagcaagc gcgacactgg
     2881 ttgacgcaag cgattgaata caagattacg gcggctcagc cggttctcgc gaatctggca
     2941 gtgcaacctc ataccactcc caattcgaaa gcagccgaac aggcaaaaaa cctcaaacaa
     3001 caaaactgaa acaatccgac caccccggta ttgcgccggt tgatacaacc ccataatata
     3061 catcctccgc tatgaacggc atcgctcgca tcaaaaccaa aacgccctct ccgtacaagc
     3121 tgactcacat cgaggtggac acgcacgaca caaagacgtt ccgatttgag cttcctgcca
     3181 atgccactct ggatatgttg cccggcgatt ttctctacgt ccatgcgacg atcaacggca
     3241 agcacgtcaa gcggccatac actccctcat cgcttgtcgg caccaccggg ttcttcgatc
     3301 tgacagtcaa gcgatatgct gctggctccg tctcgaggta cctgcacgat cagaggatcg
     3361 gagatacggt attgatgagt ggcccgaaca ccggcgggca ttgggtcgat ggaatggcga
     3421 aacgagcggg atttgttgca ggaggcaccg ggattacgcc gatgatctcc atcattcgct
     3481 ggattctgac cagcaagatt actgcggagt tattcctgat ctttgccaac aaaaccgaat
     3541 cggatgtcat cttccggcgg gaatgggaat gcaatgtcca aaactatccg aactttcatt
     3601 gccaccacgt cttggaacag ccgcctcccg ggtgggaagg tggcataggt cgcgtcaccc
     3661 ctgaaattct tcggcaccat cttcctgctc cgggctccga cacctgtgtt tttctctgcg
     3721 ggccgccgcc gatggccgac tcattggagg ccgtgctgaa agagctgggc tatccccagc
     3781 aagccattat cct
//
LOCUS       DATMTI010001049         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_336993, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001049 DATMTI010000000
VERSION     DATMTI010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336993"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VN039_16635"
     CDS             <1..673
                     /locus_tag="VN039_16635"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWV47625.1"
                     /translation="KRGARIHGAGTDMITIEGVRELHGGDHEVIPDRIEAGTYLAAAA
                     MTHGDVTATHCRPSHLEAVLMKLREAGAEVQEEKDTVRLTMPAKLKGTDIRTLPFPGF
                     PTDMQAQMVALMSLAEGTSVITETVFESRFMHVEELWRMGADIRIEGNRLVVTGCKKL
                     TGAPVMASDLRASAGLIVAGLAAEGMTQVQRVYHLDRGYERIEEKLQALGADVQRRRA
                     TMTTH"
     gene            682..1380
                     /gene="hisG"
                     /locus_tag="VN039_16640"
     CDS             682..1380
                     /gene="hisG"
                     /locus_tag="VN039_16640"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWV47626.1"
                     /translation="MLTIALSKGKLIEPALDLFRRAGYEIAGLSVDSRRLIFVSPEND
                     MTFLIVRSSDVPTYVEYGGADAGIVGKDVLMEQESDVYEPLDLGFGACRISVASLRGE
                     GSNDRLSSKIRIATKYPRISERYFNKRGIPVEIIKLYGSIELAPVVGLADRIVDLVET
                     GVTLKAHDLVELEVIARSTARFIANRASLRLKQEPLMELIRKLRAAVQNQRALSGNGR
                     PSAVGTRRKKMVRL"
     gene            1377..>2553
                     /gene="hisD"
                     /locus_tag="VN039_16645"
     CDS             1377..>2553
                     /gene="hisD"
                     /locus_tag="VN039_16645"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWV47627.1"
                     /translation="MKIVTQADRSFLPSLKKAALRGRATGAVVEKTVRTILQAVERGG
                     DKAVLRYTAQFDKVVLKAEALRVTSEEIKSAYFHIRKDEGDALRLAAERVTSFHERQR
                     TKTWMYQDGDATLGQVVGPVDAVGLYVPGGKAVYPSSVLMCAIPAKVAGVPRIVMVTP
                     PQKGGINPYLLVAADIAGVTDIYRVGGVQAVAALAYGTKTIGRVDKIVGPGNIYVATA
                     KRLLYGTVGIDMVAGPSELLVVADDEAKPAHVAADLLCEAEHDEDAQVFLVTTSERLA
                     KDVSKLIEEQLRGLQREKIASKSIARHSVAFVVSTMDEAIEIANEIAAEHLTLSVDNP
                     FDYLEKIRHAGALFLGRYTPPAVADYVAGPNHVLPTGGTARFFSPLSVNDYVKVSNIV
                     "
BASE COUNT          578 a          652 c          783 g          540 t
ORIGIN      
        1 caagcgtggc gcacgaattc atggggcagg aaccgacatg atcacgatag agggggtacg
       61 cgaactgcac ggtggagacc acgaagtgat tccggatcgt atcgaggccg gtacgtacct
      121 ggcagccgcc gccatgaccc atggggatgt gaccgcgact cactgccgtc caagccacct
      181 tgaggctgtc ctgatgaagt tgcgagaggc cggggccgag gtgcaggagg aaaaagacac
      241 ggtgcgtctg accatgccgg ccaagctgaa gggaaccgac atcagaacct tgcctttccc
      301 gggatttccc accgacatgc aagcacagat ggttgcattg atgagcctgg ccgaaggcac
      361 gagtgtcata accgaaactg tgttcgagag ccggtttatg cacgtagaag aattgtggcg
      421 gatgggagcc gatattcgta tagaaggtaa ccgattggta gtgaccgggt gcaagaagct
      481 caccggcgct ccggtcatgg cgtctgatct ccgtgccagt gccggtctga ttgtagcagg
      541 gttggctgcg gaaggcatga cccaggtcca acgcgtctat catcttgatc ggggatatga
      601 gcggatcgaa gagaaattac aagccttagg agccgacgtt cagcgccgac gggctacaat
      661 gactactcac taaggcagcc tatgctgacg atcgccctgt caaagggaaa acttatagaa
      721 cccgcactgg atctcttccg acgagccgga tacgagattg ccggattgtc ggtggatagt
      781 cgccgactga tatttgtcag tcccgagaat gacatgacct ttctcatcgt tcgttccagc
      841 gatgtgccga cttatgtgga atatgggggg gcagatgccg gaattgtcgg gaaagacgtc
      901 ttgatggagc aagaaagtga cgtctatgag ccattggatt taggcttcgg agcgtgtaga
      961 atctccgtcg cctcgctccg gggagagggt tcgaatgatc ggttgtcgtc caagattcgc
     1021 atcgcaacga agtatcctcg catcagcgag cgctatttca ataagcgcgg cattccggtc
     1081 gagattatca agttatacgg ctccattgaa ttggctccgg tagtgggatt ggcagatcgg
     1141 atcgtcgatt tggtcgaaac cggcgttacc ctcaaagcgc atgatcttgt cgaactcgaa
     1201 gtcattgccc gatcgacggc tcgtttcatc gccaatcggg cgagccttcg actcaaacag
     1261 gaaccactga tggagttgat tcgtaagctc cgagcggcgg tacagaacca gcgtgcccta
     1321 tccggcaatg gtcgcccgtc ggctgtaggc acgcgcagga aaaagatggt tcgcttatga
     1381 agattgtcac acaggcagac cgcagctttc ttccatcctt gaaaaaagct gcgcttcgag
     1441 ggcgcgcgac cggcgcggtc gttgaaaaaa ccgtccgcac aatcctgcaa gccgtcgagc
     1501 gaggcggcga caaagccgtc cttcgctaca ccgcgcaatt cgataaagtg gtactgaagg
     1561 ccgaagcgtt acgtgtcact tcggaggaga tcaagagcgc ctactttcac atcaggaaag
     1621 acgagggcga tgcgctgcgg ctggcagctg agcgggtgac gtcgtttcat gagcggcaac
     1681 ggacgaaaac gtggatgtat caggacgggg atgccacgct ggggcaggtt gtgggaccgg
     1741 tcgacgccgt cgggctgtat gtgcctggag ggaaagccgt ctacccttct tctgtgctga
     1801 tgtgtgcgat tccagccaag gtcgccggcg tcccacggat tgtgatggtc accccgcccc
     1861 agaagggcgg gattaatccc tatttgctgg tcgcagccga tattgccggg gtcaccgaca
     1921 tttatcgcgt cggcggcgtt caggccgtag cggcccttgc atatgggacg aaaaccatcg
     1981 ggcgggtcga caaaatcgtt ggaccgggga atatctatgt ggctacagcc aaacgattgc
     2041 tctacggcac agttggcatt gatatggtcg ccggtcccag tgagttgctg gtggtggctg
     2101 atgacgaggc aaaaccggcg catgtggctg ccgatttgct ctgtgaagct gagcatgacg
     2161 aggatgcgca ggtatttctt gtcacgacat ctgaacgact ggccaaggat gtgtccaagt
     2221 tgattgagga acagctcaga gggcttcagc gagaaaagat cgcgtcaaag tcaattgcgc
     2281 gccattcggt agcattcgtt gtatccacta tggacgaagc catcgagatc gccaacgaaa
     2341 tcgcggctga acatttgacg ctctcggtgg ataatccatt cgattatttg gagaagatcc
     2401 gccacgctgg agcgctcttc ttggggcgct atacgccacc ggcggtcgcc gattatgtcg
     2461 cggggccgaa tcacgtgttg ccgacaggag gaactgcacg cttcttttca ccgctctccg
     2521 tcaatgacta tgtcaaggtc agcaatatcg tgc
//
LOCUS       DATMTI010001050         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_760834, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001050 DATMTI010000000
VERSION     DATMTI010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760834"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VN039_16650"
     CDS             complement(<1..920)
                     /locus_tag="VN039_16650"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HWV47628.1"
                     /translation="MKPNSISQFIDHHYRHFNAAAMKDAAHAYRAHLDKGGKMLVTLG
                     GAMSTGELGLSLAEMIRRDNVHAICCTGANLEEDLFNLVAQKHYVRIPSYRELTAQDE
                     RVLLERHLNRVTDTCIPEAEAMRRIEAAVASEWVAADRSGLREFPHEFLYRLLRDGKL
                     KEYYQIDPDNSWMYAAAERNLPVFVPGWEDSTLGNMYAAHCLTGTIKNVHTVRTGIEY
                     MMHLAEWYPKVTEANSLGFFQIGGGIAGDFPICVVPMLSQDLRKERVPFWGYFCQISD
                     STTSYGSYSGAVPNEKITWGKLGVETPKFI"
     gene            complement(1021..3066)
                     /locus_tag="VN039_16655"
     CDS             complement(1021..3066)
                     /locus_tag="VN039_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV47629.1"
                     /translation="MTRAVPFVLALAIALSLWGKALAHDPDEPELEVPEVAVIGEKPV
                     AASSQQFIPDKEIVLQPQGRPAQVLRLIPGMVAVEHSGGAGKADQYFLRGFDADHGTD
                     VAFFADGMPINLRTHAHGQGYADLNFIIPETIEGVEVSKGAYLPEIGDFATAGAINFR
                     TRQVVQEGIAQATGAQFDTQRYLLMFSPTKEKVRTLFAVENYYTNGPFENHNRYLRTN
                     VLGKLTTSFTGRDEFSIKGTFQKSKWNASGEIPLRAVDIGMLDRFGAIDPTEGGATLR
                     ATGVMNYHYDTTTNGQFFANAYGQYYRLDLFTNFTFFLNDPVNGDGIQQSDRRVVYGG
                     DVGYKQRWEIFGIPTIGTVGVQARVDDIHPRLGTQTKRASTGTTTYSDVLSASYAPFI
                     KGEVQPASWVRFVGGLRWETFTFDVRNLCATCTLRPEGHTSSGILLPKMSMILGPWAK
                     TEFFVNYGEGFHSNDARSAVQVGGVPFARSRNYEVGIRSRPWGVEGVEFTVTAWRIDL
                     QSELVFVGDEGTTEIRGPSRRQGFEVAARGQVWGPLYINGSFTWTQAKFRNGDAIPLA
                     PEFTAYGAAILRWPEGMTSQLQATYLGVRPLIEDRSIKSPSWIVFDWSQRYRLPIKLP
                     YGRLEAFYFIQNLFNTDWEQAIFAFESRLRTEPAGVNGIHFVPGNPRTVMGGVAWHF"
     gene            complement(3192..3617)
                     /locus_tag="VN039_16660"
     CDS             complement(3192..3617)
                     /locus_tag="VN039_16660"
                     /inference="COORDINATES: protein motif:HMM:NF024355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HWV47630.1"
                     /translation="MRVCVIDGRGGGLGSRLVSGLREALQFDCQILALGTNLAAAKAM
                     REAGAEQVASGAEAISKLVPTADVIVASLNMVLPGAMLGEVTPEVAKTILEAKAKKVL
                     LPLNRVRVEIVGTEGRTMDTLIDECLSRVRVALQATCQA"
     gene            complement(3621..>3876)
                     /gene="nikR"
                     /locus_tag="VN039_16665"
     CDS             complement(3621..>3876)
                     /gene="nikR"
                     /locus_tag="VN039_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HWV47631.1"
                     /translation="VGTITFVYDHHVRDLTSKLTDIQHDYHAQILSGMHVHLDHDHCL
                     EVLVVKGKGSEIRKVADALVSVKGVKHGKLTMTTTGKGLV"
BASE COUNT          877 a         1184 c          945 g          870 t
ORIGIN      
        1 atgatgaatt tcggcgtctc cacacctaat ttcccccacg taattttttc gttcgggacg
       61 gccccggaat aggatccgta actggtggtc gaatcgctga tttgacaaaa gtatccccag
      121 aaagggaccc gctcttttcg aaggtcctga ctcagcatcg gaaccacaca gatggggaaa
      181 tcaccggcaa tcccccctcc gatctggaaa aatccgagcg aattcgcttc cgtgactttc
      241 ggataccact cggccaaatg catcatgtat tcgatgccgg ttcgcaccgt atgcacgttc
      301 ttgatggttc ccgtcagaca gtgcgccgca tacatattcc ccagcgtgga atcctcccat
      361 ccggggacaa acaccggaag gttccgttcc gccgctgcgt acatccaact gttgtcagga
      421 tcaatttggt aatattcttt gagcttccca tcccgcaaga gccgatacaa aaactcatgg
      481 gggaattctc gaaggccgct gcggtcggcc gccacccatt cggatgcgac tgcggcttca
      541 atgcggcgca tcgcctcggc ttcgggaata cacgtgtcgg tcacacgatt caagtgtcgc
      601 tcgagcaaaa cccgctcatc ctgcgcggtc aactcgcgat acgacggaat ccgcacgtaa
      661 tgcttttgcg ccacgaggtt aaagagatct tcctcaagat tggcgcctgt gcagcaaatg
      721 gcatgaacgt tgtccctgcg aatcatttcc gccaacgaca accccagttc gcctgtgctc
      781 atcgcgccac ccaaggtcac cagcattttc ccacccttgt ccaagtgagc cctgtaagca
      841 tgcgccgcgt ctttcatcgc cgccgcattg aaatgtcgat aatgatggtc aatgaattgc
      901 gaaatggaat tcggcttcat atgagactcc ttgatttgaa actgtcatca gttattacaa
      961 ccatccccca caccgtcccc tacctcttca ctaaaagaac ggtataccct gatcgtctct
     1021 ttaaaaatgc catgccaccc ctcccataac ggtgcgcgga ttgccgggca caaaatgaat
     1081 gccgttgacg ccggccggtt cggttcttag cctcgattcg aacgcaaata tcgcttgctc
     1141 ccagtctgta ttgaacagat tctgaatgaa ataaaatgct tccagacggc catacggcaa
     1201 cttgatcggc agtcgatacc gttgagacca gtcgaagacg atccaggaag gtgacttgat
     1261 gcttcgatcc tcaatgaggg gccgaacccc gagataggtc gcttgcagct gagacgtcat
     1321 tccttcaggc catctcagga tcgccgctcc ataggcggta aattccggtg ccaatgggat
     1381 cgcatcgcca ttgcgaaact tagcctgtgt ccaggtgaag cttccattga tgtacaaggg
     1441 cccccagacc tgaccacgtg cggccacttc aaatccctgc cgcctgctcg gtccacgaat
     1501 ctccgtcgtc ccttcgtcgc ccacgaacac gagttccgac tgaagatcga tccgccacgc
     1561 cgtcaccgta aactcgacac cctccacccc ccagggcctg gatcgaatac ccacttcata
     1621 gtttctcgac cgagcaaacg ggacaccccc tacctgcacg gcagaccggg catcattgct
     1681 gtggaatcct tctccataat tgacgaagaa ttctgttttt gcccaagggc ccagaatcat
     1741 actcatcttg ggcaagagaa ttccagaact ggtgtgtccc tccggcctca acgtacaggt
     1801 cgcgcaaaga ttgcgcacat caaaggtaaa cgtttcccat cgtaacccgc ctacaaaccg
     1861 aacccacgaa gcaggctgca cctcgccctt gatgaacggc gcataggagg cagagagtac
     1921 atcgctgtag gttgtggtgc cggtcgacgc ccttttcgtt tgcgtcccca gtcttggatg
     1981 gatgtcgtcc actctcgctt gcactccgac cgtcccgatg gtcggaattc caaaaatctc
     2041 ccagcgctgc ttgtacccga catcgccgcc gtacacgact cgacgatcgg attgctgaat
     2101 gccatcgccg ttgaccggat cattgagaaa aaatgtaaaa ttcgtgaaga ggtccagccg
     2161 atagtactga ccgtatgcat tggcaaagaa ctgaccgttg gtcgtcgtgt catagtgata
     2221 gttcatgaca ccggtggccc ggagcgtggc cccgccttcc gttggatcga tcgccccgaa
     2281 gcggtcgagc ataccgatat ccaccgcacg tagtggaatc tcacccgacg cattccattt
     2341 ggacttctga aaggttccct tgatgctgaa ttcgtctcgg ccagtgaagc tggtggtcag
     2401 tttccccaag acattggtgc ggaggtatcg attatggttt tcgaacggcc cgttggtata
     2461 gtagttttca acggcgaaca acgtgcgaac cttctctttc gtgggcgaga acatcagcaa
     2521 atacctctgc gtatcgaact gagcgccggt cgcctgcgca atcccttctt gcacgacttg
     2581 tctggtcctg aaattgatcg cccctgccgt cgcaaaatcg ccgatctccg ggagataggc
     2641 gcctttggac acctcgacgc cttcaatcgt ctcaggaatg ataaagttca gatcggcata
     2701 cccttgccca tgcgcatgtg tccgcagatt gatcggcatc ccgtcggcga agaaggcgac
     2761 atccgttcca tgatcggcat caaagccgcg caggaaatat tgatcggctt ttcccgcccc
     2821 gccggaatgt tcaacagcca ccatgcctgg aattaagcga aggacctgag caggcctgcc
     2881 ttgaggttgc aacacgattt ccttgtccgg aatgaactgt tgcgaagacg ccgcgaccgg
     2941 tttttcaccg atgacggcga cttccggcac ttccagctca ggctcatcag gatcatgggc
     3001 tagggccttc ccccaaagac taagcgcgat cgcaagtgcc agcacgaacg gcacggcacg
     3061 tgtcatgtcc acaattcctc ttcatgaatg ctgtggacgg atgagacgcc cacaatgatc
     3121 tctcgacagt cgactcatcg accggcgcag accctcgtgg cctcgcaact cagaacgaaa
     3181 tgggaatgtc gttatgcttg gcaggttgct tgtagggcaa cacggactcg tgagaggcac
     3241 tcgtcgatca acgtatccat cgtacggcct tcagttccca cgatttctac ccggactcga
     3301 ttcagcggca acagcacctt cttggccttc gcctcaagaa tcgttttggc gacttcagga
     3361 gtgacttcac ccaacatggc gccgggcaac accatgttta atgaggccac aatcacgtcg
     3421 gccgtcggca ccagcttgga gatcgcctcg gctcctgacg cgacttgttc agccccggcc
     3481 tccctcatgg ccttggcggc agcaaggttg gttcccaagg cgaggatctg gcaatcgaac
     3541 tgcaaggctt cccgcaaccc cgacaccagc cgcgagccca aacctccacc gcgcccgtca
     3601 atgacacaca cgcgcatgct ttagaccaat cctttgccgg tggtggtcat ggtcaatttg
     3661 ccgtgcttga cgcctttcac actcacaagc gcatcggcca cttttcgtat ttcagagccc
     3721 ttccctttca ccaccaatac ctcaagacaa tggtcatgat cgagatgcac atgcatgccc
     3781 gacagaatct gtgcatggta gtcgtgctga atatccgtca atttactggt caggtcccgc
     3841 acatgatggt catacacgaa cgtgatggtg ccgacc
//
LOCUS       DATMTI010001051         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_549640, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001051 DATMTI010000000
VERSION     DATMTI010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549640"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VN039_16670"
     CDS             <1..1390
                     /locus_tag="VN039_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47632.1"
                     /translation="GYQAISEDYVDLQYHSPPNPKTFGDFNRFTELIHSDAYLHANEY
                     GFSIDDAAGNMLEIGDGINITVGGAEGLDNTNPYDPWRFFLFNVGAAKETGPKWTKYR
                     VCTGAELPACADKAPDRDMKGTRVEDVKTKEVSIQYPAIKIGAVPGPFVIVLQDSNGA
                     LYKTEVKRLPQPPPTHPLNKVGDPPTAIDNLWNQKKEDGSYTNVKCFDNETSFDWCGG
                     LQAYSATEKTRKGALRTVYHVNTRPPEVSKPGIKFLPGDIEATLVSADRLLVKWPKAV
                     TQPPGNPVKYELTLWDRAKCPKDSNLCDGHSHNPNRACNQSQTQCEVTLPRCVPPLTA
                     ETLKSISVVAKDLKAPPGTPNAQKEVNFTAPPPKLGAIREDIHEEMDRIQKRLHDIEK
                     QNSDTKQSARKERWLEMRISVLTARLQYPTDEVLKQLEQTVEARQSLGRKAWRSFLDQ
                     LNTRIVELQSGH"
     gene            1468..>1689
                     /locus_tag="VN039_16675"
     CDS             1468..>1689
                     /locus_tag="VN039_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47633.1"
                     /translation="MRNSPTLTNALRVFIAVLLDLSLSTEGQPPSPGKRSNASDSMLP
                     RNVVISLSEVNRLFPEVTHEASTGQNLTAV"
BASE COUNT          413 a          495 c          497 g          284 t
ORIGIN      
        1 cggatatcag gcgatctcgg aggattacgt cgatctgcag tatcactcgc cgccgaatcc
       61 caagacgttc ggcgatttca accggttcac ggagctcatt cacagcgacg cgtatctcca
      121 cgcgaacgag tacgggtttt ccatcgatga cgcggccgga aacatgctgg agataggtga
      181 cggcatcaac attaccgttg gcggggccga ggggctggac aacaccaatc cctatgaccc
      241 ctggcggttc ttcctgttca acgtgggagc ggcgaaggag accggaccaa agtggacgaa
      301 gtaccgcgtt tgcacgggcg ccgagctgcc cgcctgtgcg gacaaggccc ctgaccgtga
      361 catgaagggg acgcgcgttg aggacgttaa gacaaaagag gtctccatcc aatatcccgc
      421 catcaagatc ggcgcggtcc caggcccgtt cgtgatcgtg cttcaggaca gcaacggcgc
      481 gctgtacaag accgaggtca agcggcttcc gcagccgccg ccgactcacc cactcaacaa
      541 ggtaggcgat ccgccgaccg ccattgacaa cctatggaac cagaagaagg aggacggcag
      601 ctataccaac gtgaagtgct tcgacaacga gacgagcttc gactggtgtg gaggtctgca
      661 ggcctactcg gctacagaaa agacgcggaa aggcgcgttg cgaacggtgt accacgtcaa
      721 tacgagacca ccggaggtgt ccaagccggg tatcaagttc ctccctgggg atatcgaggc
      781 aaccctcgtc tcggcggacc ggctgttggt gaagtggccg aaggccgtca cccagccgcc
      841 gggcaacccc gtcaagtacg agctgacgct gtgggatcga gcgaagtgcc ccaaggacag
      901 caatctctgc gacggccact cgcacaaccc gaatagagcg tgtaatcaat cgcagacaca
      961 atgcgaagtc acgctgcccc ggtgtgtgcc tccgctcacc gcggagacgc tcaagagcat
     1021 atcggtggtg gccaaggatc tgaaggcccc cccaggtacc cccaatgcgc agaaggaggt
     1081 gaacttcacc gcgccgcccc ccaaacttgg cgcgatccga gaggacatcc acgaagagat
     1141 ggacaggatc cagaagaggc tacacgacat cgaaaagcag aacagtgaca cgaaacaatc
     1201 ggctaggaag gagagatggc tggagatgcg gatctctgtg ctcacagccc gcctgcagta
     1261 tccgaccgac gaagtgctca agcagcttga acaaacggtc gaggcgcgcc aatcccttgg
     1321 tcgtaaagcg tggcgtagtt tcctggatca gctgaacaca cggatagtcg agctacaatc
     1381 cggccattaa gaaagcgctg cgatcattcc aacggccgga gcgtagcgag tgacctaacg
     1441 agtaggtcaa gttcgaggtt tagtgaaatg cggaattcgc caacgctgac gaacgcctta
     1501 agagtattca tcgctgtcct tctagatctc tcgctcagta cggagggaca accgccatcg
     1561 cctggcaagc gttccaacgc ctcggactca atgctccctc gcaatgttgt catctcgtta
     1621 agcgaggtga accggttgtt cccggaagtc acgcacgaag cgagtaccgg gcagaacttg
     1681 actgcagtc
//
LOCUS       DATMTI010001052         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_760979, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001052 DATMTI010000000
VERSION     DATMTI010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760979"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VN039_16680"
     CDS             <1..545
                     /locus_tag="VN039_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47634.1"
                     /translation="PRTAAGMLAWTGPVAEVFAFDNFVDALANWPHREGKEGVVVRSG
                     DKMVKLKQPDYLELHKLVTNASPKTVWQQLSIGKSRDEIISAFPDEFHDYIGSMIEPL
                     EKAYEARLEQILSGYSDATNEVVLGTWGYDNPDTPTRKMYANVFSKYPDKRYYFLLLD
                     NKPIRTVLWQELRPREVEVR"
     gene            999..>1816
                     /locus_tag="VN039_16685"
     CDS             999..>1816
                     /locus_tag="VN039_16685"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV47635.1"
                     /translation="MATLTICRGISGSGKSTWARQQNAIVVCRDDIRDMLFETHKQDP
                     HEYYAQSKDALSACESSVTVVQESMISGLLRAGKDVIVDNTNIEFKFVKQIAKIGYRH
                     GADVTVKLFDVPLTDAIIRDQTRGAIGGREVGREVITRQYNRLKSNKSMTLPEVHYPR
                     PYSGTPGKPRAFMVDIDGTLADYKNYRGPYDLNVGVDDVREKVADVVAELGNQYHVIV
                     MSGRKEDCREDTIAWLEDNGIPYNQLFMRRSNDNRADNLIKADLFDEFVRDNFDV"
BASE COUNT          459 a          435 c          493 g          429 t
ORIGIN      
        1 gccctcggac ggcggcagga atgcttgcct ggaccggccc ggtggcagag gtatttgctt
       61 ttgacaactt cgtagacgcc ttggcaaact ggcctcatcg tgaaggcaaa gagggggtag
      121 ttgtccgcag tggcgacaag atggtcaaat tgaagcagcc tgattatcta gaactacaca
      181 agttggtcac caatgcatcc ccgaagacag tctggcagca gttgtccatc ggcaagtcac
      241 gcgatgagat aatctctgcg tttccagacg agttccatga ctatatcggt tcgatgattg
      301 agcctttgga aaaggcatac gaggctcgac tggaacaaat cctgtctggc tactcagatg
      361 cgacgaacga ggtggttctt ggtacctggg gctatgacaa tccagacact ccaacccgga
      421 agatgtacgc caacgttttc agcaagtatc ctgacaagcg ctactacttt cttctgcttg
      481 acaacaagcc gattcgtacg gtactatggc aagaacttag accgagagag gttgaagtcc
      541 gctagtccta gcgaacagaa cttttgagtc ttactttctt ggcctagacg cttgacaggg
      601 tgtggcctca tctgatacgg tgaggctcca ccaccattta ggagtcaaca tgtgcgagca
      661 cgatgaggaa atgcgtaggg agtgggccga ttgggaccgc tccgtcaccg gagtgctttg
      721 tggcaacgat gctccgcacg caccacatga gggctgtctt ggcatcatgt gcgactacgg
      781 ctgcaacgcc gaatactttt aggcgaaacg atgaccagag ttgagattac ccgtggcccc
      841 gagaagggta tgcgtggcac tcttcttgat gagaagccaa caaccaacaa cgatgacgtt
      901 ttggtcaacc tcgactcttc ctatggtcgt gtgaattcac gattcaacag gaccctttca
      961 tatacttacg catctatccg aattatcgag gagtaatcat ggctacactg acgatttgcc
     1021 gaggcattag tggctcaggt aaatcaactt gggctcgcca gcagaatgcc attgtcgtgt
     1081 gtcgtgacga cattcgagac atgctcttcg agactcacaa gcaagaccct cacgaatact
     1141 atgcgcagag taaggatgct ctgtctgcat gtgagagcag tgtgaccgtg gttcaggagt
     1201 cgatgatttc tggactgctg cgcgccggta aggatgtgat tgtcgacaac acgaacatcg
     1261 agttcaagtt cgtcaagcag attgctaaaa ttggctatcg tcatggtgcc gatgtgactg
     1321 tcaagttatt cgatgtgcca ctgacagacg ccatcattcg tgaccagaca cgaggcgcaa
     1381 tcggcggcag ggaggttggc cgtgaagtta tcacgaggca gtacaatcgt ctcaagtcca
     1441 acaagtctat gactctgcca gaagtacact atccgaggcc atattctggt acccccggca
     1501 agccaagagc attcatggtc gacattgatg gaactctggc cgactacaag aactatcgtg
     1561 ggccatatga cctgaatgtg ggagttgacg atgttcgtga gaaggttgcc gatgtggttg
     1621 ctgagttggg taaccaatac cacgtgattg tcatgtccgg gcgaaaagag gactgccgcg
     1681 aggataccat tgcctggctg gaagacaacg gaattccata taaccaactg ttcatgcgta
     1741 ggtctaacga caatcgtgca gacaatctga ttaaggcaga cctgtttgat gagttcgttc
     1801 gagataactt cgatgt
//
LOCUS       DATMTI010001053         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_253449, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001053 DATMTI010000000
VERSION     DATMTI010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253449"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VN039_16690"
     CDS             complement(<1..1086)
                     /locus_tag="VN039_16690"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HWV47636.1"
                     /translation="MTDGILDGYETDCQFVAYLGTDRLSGEMVQMPISRFNRHGLVAG
                     ATGTGKSRSIQQIAEWCSQAGVPVLLCDGKGDMSGLAREGNPSDKARKRMELTGQDTW
                     WEGNSFPVQFLAPGGQGNGVPVRVTVGSFQYKSLAKIIGLTPAQTNALGSVFMYARRA
                     KAELETIEDLQQYVRLVRDDPESSISSGVCNRIYDALNLFEENNPGLFGGPEFDVMDL
                     LKVSSSGEHVWGNVSIIDSSQLVDNPDVLTTFLLWLMAQLNSALPEVGDQDKPRLVFF
                     FDEAHMMFQDAPKEFTRGIVRLVKSIRSKGVGIFFISQSGADIPDAVLAQCGNRIQHT
                     LRANTPAQLRDLKKTADTFPISSRYDIA"
     gene            complement(1187..>1636)
                     /locus_tag="VN039_16695"
     CDS             complement(1187..>1636)
                     /locus_tag="VN039_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47637.1"
                     /translation="SSKLEEALSAALPSYSQGFTVEVGTPESDADGTGFVPAPRNTPA
                     GITGLTTAQIARDTRSVRVQSAPVAHDGEKRIGRPKTLEGYAASDGGPVREWGTRSPG
                     ELKRQGVKVYRPKGSHVAPEPEAEPEVHEPVARPVAKKAAPVVLRRK"
BASE COUNT          328 a          514 c          456 g          338 t
ORIGIN      
        1 cgcgatgtca taccgcgagg aaatcgggaa ggtatcggcg gttttcttca ggtcccgcaa
       61 ctgagccgga gtgttggccc gtagcgtgtg ctgaatgcgg ttgccgcact gagccagcac
      121 agcatccggg atgtcggctc cggactgcga gataaagaag atacccacgc ccttggaccg
      181 gatgctcttg accaaccgga cgatgccgcg cgtgaattcc ttcggcgcat cctgaaacat
      241 catgtgcgcc tcatcaaaga aaaacaccaa tcgcggctta tcctggtctc caacttccgg
      301 cagggcactg ttgagctgtg ccatgagcca gagcaggaag gtggtcagca cgtccgggtt
      361 atcgaccagc tgtgagctgt cgatgatcga gacgtttccc cacacgtgct cgccactact
      421 ggacaccttg agcaagtcca tgacatcgaa ctctggaccg ccgaacagac caggattgtt
      481 ttcctcgaac aggttcaacg catcgtagat gcggttgcac actcccgagg aaatagagga
      541 ctccggatca tcccgcacaa gccgaacgta ctgctgcaaa tcctcgatcg tctcaagctc
      601 agctttcgca cgccgcgcgt acatgaacac gctgccgagt gcgtttgtct gagccggagt
      661 aagaccgatg atcttagcta gtgacttgta ttggaagctg ccgaccgtga cgcgaacagg
      721 gacaccgttc ccctgtcctc ccggagccag gaattgcacg gggaaggaat taccctccca
      781 ccatgtgtcc tgtcccgtga gttccatgcg cttccttgct ttgtcggaag ggttcccctc
      841 ccgcgcaagt ccgctcatgt cccctttacc gtcgcagagc agaaccggaa cgccagcttg
      901 cgaacaccat tcggcaatct gctgaatgga acgggacttt ccggtacccg tagcgccagc
      961 gacaagcccg tgacggttga atcgggagat cggcatttgc accatttcac cggacaaccg
     1021 atcggtaccg aggtaggcaa cgaactgaca gtcagtctcg taaccgtcca gaatgccatc
     1081 cgtcattttt ctgcctttcg tttgggtttt ctgtctgatc ctgcgtaacg cagaaccatg
     1141 ccatgccacg gcttgcaccg tggcacaaca aagcactacg tgatgattac ttgcgtcgca
     1201 gcactactgg cgcggccttc ttcgcgaccg gacgggcaac gggctcgtgc acttccggct
     1261 ctgcctccgg ctccggagcg acgtgcgaac ctttcggccg atagaccttc accccttgcc
     1321 tcttgagttc tcccggggag cgcgttcccc attcgcgaac cggcccaccg tccgaggctg
     1381 cgtaaccctc aagcgtcttg ggccgaccga tgcgcttctc tccgtcgtgc gcgaccggag
     1441 ccgactggac acgcacagaa cgcgtgtcac gggcgatctg cgccgttgtg agccccgtaa
     1501 tccctgcggg ggtgttcctg ggcgcgggaa cgaatcccgt gccgtcagcg tcgctctctg
     1561 gggttccgac ttccaccgtg aacccctgac tgtaagaagg gagcgctgcg gagagagctt
     1621 cctccaactt ggaaga
//
LOCUS       DATMTI010001054         3830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_337205, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001054 DATMTI010000000
VERSION     DATMTI010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337205"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..2377
                     /locus_tag="VN039_16700"
     CDS             206..2377
                     /locus_tag="VN039_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HWV47638.1"
                     /translation="MNNLGLQQAHNTSAGSVNGSLADSGLTCLLIIARFHDLPADGSQ
                     LRHQFARSGQVLSDAELLHAAKHLGLKAGLLKTTWSKLQGMPLPAMAKRVDGRYVVLA
                     KVEGEKVLIQDPLEARPLVLSHEQFEATWTGDLLLFTKRAPVRLQDLTFDFTWFIPAI
                     VKYRKFFGEVLIASFFLQLFALLTPLFTQVVIDKVLVHKGFTTLHVLAIGMITLAIFE
                     AIVGGLRTYLFSHTTNRIDVSLGAQLFRHILALPISYFEARRVGDTVARVRELEQIRQ
                     FLTSQSVTVVLDVVFTVVFLTVMWVYSPTLTLVVMASLPVYALLSIAITPAIRARLHE
                     KFNRGAENQAFLVEAVSGIQTVKAMAVEPPLVRRWEEQLAGYVRASFRATSVMMIAGQ
                     SATCVQKVTTVAVLWLGAYRVIDGNLSIGQLIAFNMLSAQVTGPLLRLVNLWQEFQQV
                     GISMQRLGDVLNTQPEPSYNPNRTTLPRVIGQVRFEEVTFRYRPDGPEVTRKMSFSVE
                     AGHMIGIVGRSGSGKSTIAKLLQRLYVPERGRILVDGVDLAQVDPAWLRRQVGVVLQE
                     NFLFNGSVRSNIALTDPGLAMDQVIQAATMAGAHEFIMELAEGYDTLIGEHGCTLSGG
                     QRQRIAIARALIANPRILIFDEATSALDYESEAVIQRNMAQICQGRTVFIIAHRLSTV
                     RPAHRIYVVDKGEIVEEGSHDELLRLDGFYARLQVHQIGKG"
     gene            2389..3780
                     /locus_tag="VN039_16705"
     CDS             2389..3780
                     /locus_tag="VN039_16705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HWV47639.1"
                     /translation="MAGTFGRHWTVWKAAWQAESSRPDGSAVSGGQAMEFLPAVLEIQ
                     QVPPSPIGRALLWTILAAFTAAGLWATLGWIDIVATAQGKIIPSGYSKIIQPYEAAVI
                     ASIQVQDGQAVKQGDVLIELDSTLNRADYDRAFNEYRATKVDVARLKALIKGRATFEA
                     PSDGNGPYVVLQQHLLRDQLAEYQAKIAAAQHLVDQRQAALEQTKENILRLEATVPME
                     TERAEAYKKLLEHEAVTKMDFLQAEGQRIDKAQELAGQKQKLQQDRAALAEAEKHYRA
                     MASEFQQTKQAELSALETKAASLAQEVTKAGQKAGLQRLTSPIDGVAQQLAVHTVGGV
                     VTPAQQLLIVVPQDHPVEVEVQVENKDVGFVKEGQPVEVKVETFQFTLYGTIPGHVLT
                     VSDDAASLDKIGLVYPTRISMDRSTIQVDGKQVNLSPGMAVTVEIKTGQRRIIEYLLS
                     PLLKSMKESLRER"
BASE COUNT          846 a         1010 c         1140 g          834 t
ORIGIN      
        1 actgtccccc ctcaaagaga agtcagaaca gttccgatcc attgctacac tcggcgcgca
       61 gaccattacg tagcgatttt atcccaaggc cgtggatcga atgctcggaa aggtttcgaa
      121 ccaggttaat tgttcgcaaa agtggtagtc acgtcacgcg gtaaccattt ctccaactcg
      181 ctgagtcggt acaacggtag atcccatgaa caatttggga ctgcaacagg cgcataacac
      241 ttccgccggt tccgtaaatg ggtcgttggc cgacagcggt ctgacctgtc tcctcatcat
      301 cgctcgattc catgatcttc ccgccgatgg ctcacagcta cggcatcagt ttgcacggtc
      361 ggggcaggtt ctctccgacg ccgagcttct ccatgccgct aaacatcttg ggttgaaggc
      421 tgggctactc aagaccacat ggagcaaact ccaggggatg ccgctgcctg ccatggcaaa
      481 gcgggtcgat ggccgctatg tcgtgctggc gaaggtcgaa ggagagaagg tactgatcca
      541 ggatccgctc gaagcacggc cgctcgtcct ctcacatgag caatttgaag cgacatggac
      601 cggggacctg ctgctcttta ccaaacgagc ccccgttcgt ctgcaagatc tcacgttcga
      661 tttcacctgg ttcatccccg ctatcgttaa ataccgaaag ttcttcggag aggtgctgat
      721 cgcgtcgttc tttcttcaac tctttgcctt gttgacgccg ctcttcacgc aagtcgtcat
      781 cgacaaggta ctcgtacaca agggcttcac gacgctccat gtgttggcga tcggcatgat
      841 cactctggcg atcttcgaag cgatcgtcgg cgggctccgg acctacttgt tttcgcatac
      901 gacgaatcgg atcgatgtca gtctgggcgc ccaactcttc cggcatatcc tggcgcttcc
      961 catatcctat tttgaagctc gacgcgtcgg cgatacggtc gcccgtgtgc gtgagttgga
     1021 acagatccga caatttttaa cgagtcaatc cgtgacggtg gtgctggatg tcgtgttcac
     1081 cgtcgttttt ctgacagtca tgtgggtata cagccccacg ctcacgctcg tcgtcatggc
     1141 ttcgctcccg gtctatgcgt tgctgtcgat cgccattact ccggcaatcc gagcgcgctt
     1201 gcatgaaaag ttcaaccgag gcgccgaaaa ccaggcgttc ttagtcgaag cggtcagcgg
     1261 gatccaaacc gtcaaagcca tggcggtcga acctccgctg gtgcggagat gggaagaaca
     1321 actggccggc tatgtgcggg cgagctttcg cgcgacgagc gtgatgatga tagccggtca
     1381 atccgcaacc tgtgtccaaa aagtgaccac cgtcgcagta ctgtggctgg gggcctatcg
     1441 tgtgatcgat ggaaatctga gcataggcca gttgatcgca ttcaacatgc tgtcggccca
     1501 agtgacaggg ccacttctgc gattggtgaa tctctggcag gaatttcagc aagtgggcat
     1561 ctcgatgcaa cggttgggtg atgtgctcaa cactcagcct gagccgtctt ataacccgaa
     1621 tcgaacgacg ttgcctcggg tcatagggca ggtacgattc gaggaagtca cgtttcggta
     1681 ccggccggat gggccggaag tcaccaggaa gatgtcgttt tcagtagagg cggggcacat
     1741 gatcggtatt gtcggacgtt caggatcagg aaaaagcacc attgccaaat tgctgcagcg
     1801 tctctatgtg ccggagcggg ggcgcatttt ggtggatgga gtggatttgg cgcaggtcga
     1861 tcctgcatgg cttcggaggc aagtgggagt cgtgctgcag gaaaatttcc tgttcaacgg
     1921 ctcggtccga agcaacatcg cgctgactga tccggggtta gcaatggacc aggtgatcca
     1981 ggcagcgaca atggccgggg cccacgagtt catcatggaa ttggcggaag gttacgacac
     2041 gttgatcggc gagcacggat gcacgctctc aggaggccaa cgtcagcgga tcgccattgc
     2101 acgggcctta attgcgaatc cccgtattct gatcttcgat gaggcgacca gcgcgttgga
     2161 ctacgaatcc gaagcggtga tccaacggaa catggcgcag atctgtcagg gacgtacggt
     2221 ctttatcatt gcgcatcggc tgagtacggt tcgacctgcg catcgcatct atgtcgtcga
     2281 caaaggagag atcgtcgagg agggatcgca tgatgaactc cttcgtcttg acggtttcta
     2341 tgcacgactc caggtgcatc aaataggcaa ggggtagagg tctctcagat ggctgggacg
     2401 ttcggcagac attggacggt gtggaaggca gcgtggcagg ccgaatcaag ccgaccggac
     2461 ggttccgctg tctctggcgg acaggccatg gaatttctgc cggcggtgtt ggaaatccag
     2521 caggttcctc catctccaat cggtcgagcg ctgctctgga cgatcttggc cgcgttcact
     2581 gcggcagggt tgtgggcgac gttgggatgg atcgatattg tggccacagc gcagggcaag
     2641 attattccca gcggctactc gaagatcatc cagccgtacg aagcagcggt cattgcatcg
     2701 atccaggtgc aggatggaca agccgtgaag cagggcgatg tgctgatcga gctcgattcg
     2761 accctcaacc gtgccgacta tgatcgggcg ttcaatgaat atcgagcgac gaaagtggat
     2821 gtggccaggt tgaaggcttt gatcaagggg cgagccactt tcgaggcacc ttctgatgga
     2881 aatgggccct acgttgtgct gcaacagcac ttgctgcgtg accaactggc cgagtatcag
     2941 gccaagatcg cagcagcaca gcatcttgtc gatcaacgtc aagctgcgct cgaacaaact
     3001 aaagagaaca tcctccgttt ggaagccacg gtgccgatgg agaccgaacg tgcggaagcc
     3061 tataagaaat tacttgaaca cgaagcagtc accaagatgg actttctgca ggccgaaggg
     3121 caacggatcg acaaggcaca agagttggcc gggcaaaagc agaagttgca acaggatcgg
     3181 gcggcgcttg cggaagccga aaagcactac cgggccatgg cgtcggagtt tcagcagacg
     3241 aaacaggcgg aactgtcggc tcttgaaacc aaagctgcct ctcttgcgca agaagtcacg
     3301 aaggctggtc aaaaggccgg tttgcagcgg ctgacctcgc cgatcgacgg cgtcgcgcag
     3361 cagttagcgg tgcataccgt cgggggcgtc gtcactccgg cgcagcagct gctcattgtc
     3421 gtgcctcagg atcatccagt cgaagtggag gtgcaagtcg agaataaaga tgtggggttt
     3481 gtgaaggaag gacagccggt cgaggtcaag gtcgaaacct tccagttcac gttgtatggc
     3541 acgattccgg gtcatgtgct gacagtttct gacgatgcgg cttctctcga taagatcgga
     3601 cttgtctacc caaccagaat cagtatggat cgatcgacga ttcaggtcga tggcaagcaa
     3661 gtgaatcttt cgccagggat ggcggtcact gttgagatca agaccggtca gcgccgcatc
     3721 atcgaatatc tcctgagtcc gttgctcaaa tccatgaaag aaagtctgag ggagcggtaa
     3781 cggatggtcc catcgccggt gctgctcggg ctctgtctgc ttgtcgtccc
//
LOCUS       DATMTI010001055         1791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_633501, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001055 DATMTI010000000
VERSION     DATMTI010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633501"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VN039_16710"
     CDS             complement(<1..426)
                     /locus_tag="VN039_16710"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWV47640.1"
                     /translation="MQARMTNPAMIVPNAMPALQALGTVLIKSGAPPRLLELAHLRAS
                     QINGCSVCVDMHASFLKKAGETDERLFAVAAWREAPYFTDAERAVLALTEAVTRLSDQ
                     ANAVPDEIWDEAARHFDEPVLAALILSIATVNLWNRLHVA"
     gene            547..1431
                     /locus_tag="VN039_16715"
     CDS             547..1431
                     /locus_tag="VN039_16715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV47641.1"
                     /translation="MEHEWLTERFEENRSHLQAVAYRMLGSLNEADDAVQEAWLRLSR
                     SDTNSIENLRGWLTTVVGRVCLDMLRTRTSRREEPLDAHAPETMASPADVSNPEQEAL
                     LAESVGLALLVVLDTLAPAERLAFVLHHMFDLPFNEIARIMGRSPASARQLASRARRR
                     VQGVPAASRADLARQREVIEAFIAATHAGDFGALLEVLDPDVVRRIDVAAKSGASKEV
                     RGAQVVAKGALAGARHAQSSQIALALVNGNVGIVVVSQRRLSMAITFVIREGKIAEID
                     LITSPARLRQLDLAVFND"
     gene            1570..>1791
                     /locus_tag="VN039_16720"
     CDS             1570..>1791
                     /locus_tag="VN039_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWV47642.1"
                     /translation="MKYVCLGHLDENAWMTLSESERNTFLDECFAYDDVLRKNGHFVG
                     GEALQSARNATTLRWKNGKVMITDGPYAET"
BASE COUNT          389 a          488 c          544 g          370 t
ORIGIN      
        1 agcaacgtgg aggcggttcc acaagttgac ggtggcaatg gaaaggatca acgccgccag
       61 taccggctca tcgaagtgac gggcggcctc atcccaaatc tcgtccggca cggcgttcgc
      121 ctgatcactg aggcgggtca cggcctccgt aagcgccagg accgcacgtt cggcatcggt
      181 aaaatatggc gcctccctcc aggctgccac ggcaaagagc cgctcgtctg tttcaccggc
      241 tttcttcagg aaactggcat gcatatcgac gcagacgctg caaccgttga tctgactcgc
      301 gcgtaggtgg gcaagttcga gcagtcgagg cggagcaccg ctttttatta agacggtccc
      361 gagcgcttgc agtgcaggca tcgcattagg aacgatcatc gcggggttgg tcatccgtgc
      421 ttgcatgata ggcctccttt aaggttatgt ctgccgagcg gggatgcgga atatccatct
      481 ccctgtcaca tcggctaggc ttcattcgtc atagctatga cggatcacaa ggaggaaatg
      541 tgacgaatgg aacacgaatg gctgacggaa cgatttgagg agaaccggag tcatcttcag
      601 gcggtggcct atcgaatgct cggctcgctg aacgaagccg atgatgcggt gcaggaagcc
      661 tggctccgcc tcagtcgctc cgatacgaac agtatcgaga atctcagagg atggctgacg
      721 acggtggtcg gcagagtatg tctggacatg ctgcgtaccc gcacgtcacg gcgtgaagag
      781 ccgttggatg cgcatgcacc cgagaccatg gcgagtcctg cagatgtaag caatccggag
      841 caggaagcat tgctggctga atcggtcggg ctcgcattgc tcgtcgtcct agacacgctg
      901 gctcctgccg aacgactcgc gttcgtgctg caccacatgt tcgaccttcc ctttaatgag
      961 atcgcccgga ttatgggaag gtctccggct tcggcgagac aactcgcgag ccgggcccgt
     1021 cgccgggtgc agggtgtacc ggcggcttcc cgcgccgacc tcgcacgcca gcgagaagtc
     1081 attgaggcct tcattgctgc cacgcacgcc ggcgactttg gcgcgcttct cgaagtactg
     1141 gacccggacg tggtgcgtcg aatcgacgtt gccgccaagt cgggcgcatc gaaagaggtg
     1201 cgtggtgcgc aagttgtggc caaaggggca ctcgccggtg cgcgacatgc ccagtcgtca
     1261 caaatagcgc tcgcgctggt gaacggaaat gtgggaatcg tagtggtttc gcaacggcgg
     1321 ctgtcgatgg cgattacatt cgtgatcagg gaagggaaga tcgctgagat cgacctgatc
     1381 acttctcccg cacgcctccg tcagttggac ctagcggtct tcaacgactg aggaccctac
     1441 cgctattgcg gttcggataa gctttgaaag ttccggacca atcgacgtat gtgtcgaggc
     1501 tgtcgtcgat ttttgtcttc ccgaacgact attcaacata gacggttatt caacaagcat
     1561 ggaggaacta tgaaatacgt ttgcctcgga caccttgacg aaaatgcatg gatgacgctg
     1621 tcggaaagcg agcgaaatac ctttttggat gaatgtttcg cctacgacga tgtgcttcga
     1681 aagaacggac actttgtcgg cggagaggcc ctccaaagcg ctcggaacgc cactacatta
     1741 cgatggaaga acggtaaggt catgattacg gacggcccat atgcggaaac c
//
LOCUS       DATMTI010001056         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_761055, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001056 DATMTI010000000
VERSION     DATMTI010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761055"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..963)
                     /locus_tag="VN039_16725"
     CDS             complement(232..963)
                     /locus_tag="VN039_16725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWV47643.1"
                     /translation="MEAIQVGPPSVADVLPLRVVIGWMGLFIAAGFGVAGLLCWPYGQ
                     DLVRRKQDGAPSAYDGVTGLPTRRLFLVLLKQALSRAETTKRHVAVLVCALEQFRPLS
                     AALAAPNMTLVVRVQAARIKSALRPHDVVARLDEYTFAVIADNLESPDEARLIAGKIQ
                     KAIALPLLVEGQELLTSCRTGGIVGPQSGTDAESFLDAASRALSESQTADESIAFVSD
                     PATTTSSSSSLTSAGRMRHYAGARH"
     gene            complement(1300..>1604)
                     /locus_tag="VN039_16730"
     CDS             complement(1300..>1604)
                     /locus_tag="VN039_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV47644.1"
                     /translation="MISVDDLSLHEPDGHKMVKMLTDTHTPDPLSSLVNERERASLAG
                     AIQSLPEKERLVLTLYYYEELTMKEIGELLKVTESRVCQIHTKAIIRLKAHLQKPN"
BASE COUNT          362 a          467 c          431 g          344 t
ORIGIN      
        1 cctaaccatc caggcgacat gcccgaaggc actctgcgtg ctattctcaa gcaagcaggc
       61 gtgttaccag ccgagttcat aggcggctga cagcaaagat tttttagaat acggtcttac
      121 acagttcctc atccagcctc ccgaccacgc gcaatcgtcc aaatgcgcct tgatgcggtg
      181 acggtaggct atgtaatctg gcgtgctttc tactttttat tagtaatgag actaatgccg
      241 tgcgcctgcg tagtgacgca ttcggcctgc agaggtcagg ctcgacgacg atgaagtagt
      301 tgtagcggga tccgacacaa atgcaatgga ctcatcggct gtttgactct ccgagagggc
      361 tcgtgacgcg gcatcaagga atgactcggc atcagtccca ctttgtggcc ccacaattcc
      421 tccagttcgg catgacgtca atagctcctg gccttcgacc aacagtggaa gtgcgatcgc
      481 cttctggatt ttcccggcga tcaatcgagc ttcgtccggt gattcgaggt tatcggcgat
      541 cacggcgaag gtgtattcgt ccaagcgcgc caccacgtcg tgcggtcgca atgcgctctt
      601 gatgcgggca gcttgcactc gcacgaccaa ggtcatattc ggggcagcca aagcagccga
      661 cagcgggcgg aactgttcca gggcacatac caagacagca acgtgccgct tagtcgtctc
      721 agccctgctc agcgcctgtt tgagaagtac aaggaaaagc cgccgggttg gaagaccggt
      781 cacaccatcg tacgccgacg gcgcaccatc ctgcttgcgc cgtacgaggt cctgaccata
      841 cggccaacac aagagccccg ccacaccgaa tccggcggcg atgaaaagcc ccatccatcc
      901 gatgacaact cgcagaggga gaacgtcggc cacgctcgga ggaccgacct gtatcgcctc
      961 catccacacc atacttgcac cgcaaaccgt tgctgaggca caccaccatg ctagagagcg
     1021 cggtcgcgat cgtgagggcc cagcgtgtga taccttccgg tgcatcgtcg tgattacgga
     1081 ttgaagatcg acttgagttc ggaatagacc tgagacccga tccacgttcc ggcccgatgc
     1141 gatgaatgac cactccatcc accaggccct tgtcgaggtc tcgtccagta aggtatcggt
     1201 ttctccgact cagaactgaa ggtgggcagc gcacaacacc tcggatggta tcagaacgaa
     1261 tacaaatgaa cggccgactc agtcggtcgg ccgattcgat caattcggtt tttgcaagtg
     1321 tgccttgagc ctgatgatgg ctttggtatg gatttggcag acccgtgact cagtgacttt
     1381 cagcagctcg ccgatttcct tcattgtgag ttcctcatag taatacaacg tcaggacgag
     1441 ccgttctttt tccggcaacg attgaatggc cccggcaagc gatgcacgct cacgctcgtt
     1501 gaccaaagag gaaagaggat cgggcgtgtg cgtatcggtc aacatcttga ccattttgtg
     1561 cccgtccggc tcatgaagac ttaaatcgtc cacgctgatc atca
//
LOCUS       DATMTI010001057         1765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_253511, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001057 DATMTI010000000
VERSION     DATMTI010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253511"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VN039_16735"
     CDS             complement(<1..683)
                     /locus_tag="VN039_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV47645.1"
                     /translation="MADKDDQQPKASDHQTILVAHMMTPGVVQIPGDVSVTEAASLLE
                     RERMPCLLVKDTEARFGLMTPTDIVKKVVAQGLEPDHIEVRTIMTRPVQFIEYDRAMD
                     EASALMMSTGTPILIVTKQDQPVGVLTARDLILSPKRCATNISATISVIEGEGVGAEH
                     QVAILQLSHAGASVESPALLLPGTKVILSFCLSEMMSPLTIRGAVLTNARVEPVSGTT
                     GSFMAASRGV"
     gene            828..>1765
                     /locus_tag="VN039_16740"
     CDS             828..>1765
                     /locus_tag="VN039_16740"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HWV47646.1"
                     /translation="MPPSKSEMMAPNASYHREFIRRLRTIPVHGLGLSVDIHAPDLAS
                     LRRSLQERQVPPDYLEVFRTTPMALASARKEAGNGLLTYHGEGLWIIQPGMVDSTAFR
                     QEIAEAAEQLMILQSAWLNHECATKYLAGYYYGTYLPPLYTPLSAKVVADNTRLIQNL
                     LDQQCRLANGSTPLVLLEMPPLTYFVAGTMSIPRFFRVISEQAPCGLVLDVGHLWTVF
                     RYSGAHLDMPLTRFVEEFLNEFPMDRVVEIHVAGLAVHESHRILDSRLKGGMNDDVLP
                     AWIDVHAAPIPTVLFEMLDQILCHSQLTSLKGLALEV"
BASE COUNT          380 a          475 c          480 g          430 t
ORIGIN      
        1 acaccccgag atgccgccat gaaggaaccg gtggtgccgg ataccggctc aactctggcg
       61 ttagtcaaga cggcccctcg aatagtcaag ggactcatca tctcagagag gcaaaagctc
      121 aatatgactt tggtccctgg cagcaacagc gcgggagact ccaccgaggc gccggcatgg
      181 ctcagttgta agatggcaac ctgatgctca gctcccacgc cttctccctc aatgacactg
      241 atggtggcgg agatgttcgt cgcacatcgt tttggagaaa ggatcagatc tctcgcagtg
      301 aggacaccga ccggttgatc ttgtttcgtc acgatgagga tcggtgtccc ggtggacatc
      361 ataagtgccg aggcctcatc catcgctcga tcgtactcga tgaattggac cggtcgtgtc
      421 atgatggtcc ggacttcgat gtgatccggt tcgaggccct gcgcgacaac ctttttcaca
      481 atatcggtcg gcgtcatgag tccgaaccga gcctctgtat ctttgactag gaggcagggc
      541 attcgctcac gttccaagag cgatgccgcc tcggtgaccg atacatcgcc tggaatctgg
      601 actacgccgg gtgtcatcat gtgtgcaacc agtatggtct ggtgatccga ggctttgggc
      661 tgctgatcgt ccttgtccgc cattcctcag cgaggtcctc tttcctcacg aatctgcccc
      721 cctcctggct gcaatgtatg caaagtatag cagaaggtac ttggaagcca tgagaggttg
      781 aaagccttgt cattcaatgg tttgacgaca tacactagga atgcattttg cccccgagca
      841 agagcgaaat gatggcaccg aatgcgtctt accataggga gttcattcgt cggctccgga
      901 caattccagt gcatgggctg gggctttccg tcgatattca tgctccggac ctcgctagct
      961 tgcggcgaag tctacaagaa cgtcaagtgc cgcctgatta ccttgaagtg ttccgtacga
     1021 cacccatggc tttggcatct gccagaaaag aggcaggcaa cggtctgctg acatatcacg
     1081 gcgaagggtt atggatcatt cagcccggga tggtcgactc tacggcgttt agacaggaaa
     1141 tcgccgaggc tgcagaacaa ctgatgatct tgcagagtgc ctggctgaat catgagtgtg
     1201 cgacaaaata tctcgccggc tattattacg ggacctatct tccccccctc tatactccgt
     1261 taagcgcgaa ggtggtagct gataatacac gattgatcca aaatctgctc gatcaacagt
     1321 gccggttggc caacggaagc acgccgttgg tattgcttga aatgccgcca ctcacctatt
     1381 ttgttgcagg gacgatgtcc atcccgaggt tttttcgggt catcagcgaa caggcaccct
     1441 gtgggctggt cttggacgtg ggccatctct ggacggtgtt tcgatattcc ggagcacatc
     1501 tggacatgcc ccttacacga tttgtcgagg aatttctcaa cgagtttccc atggatcgtg
     1561 tggttgagat tcatgtggcg ggtctggccg ttcatgaatc acaccgaatc ctcgattcac
     1621 gactgaaggg tggtatgaac gatgacgtgc ttcctgcctg gatcgatgtc catgctgccc
     1681 ccattccgac cgtcttgttc gagatgctcg accagattct atgtcattct cagctcacca
     1741 gcctgaaggg ccttgccttg gaagt
//
LOCUS       DATMTI010001058         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_549819, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001058 DATMTI010000000
VERSION     DATMTI010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549819"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2834
                     /locus_tag="VN039_16745"
     CDS             <1..>2834
                     /locus_tag="VN039_16745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta''"
                     /protein_id="HWV47647.1"
                     /translation="GEVTSKLPTREFRTRHGDIVEMTTRDCTLEVKTTDGKTDKYNLP
                     YNTLLMVTTGDTVKKDQVFATFEPTAGRRTEKATKDITANMSGEVWFDGFDADEKRDR
                     QGNISRTANRPGTIWVLGGDVYNLPTGARHLVQDGQVLNVGEMIAETHVVSEHGGEVR
                     IPADLEIEEQKVGKEVVKKIVNGKELTIIIAGITPTNADLVQTKKEQLWKVGEDEETY
                     IVKSPEGTTLENGSIMAELIDESMSVPTSGQIRYLDVEVDEQRILTQPGKILFLPEEI
                     HQVSKDISLKVPGIESGMEVTAGTEIVKGVTTQTDGIVEIVEENDIIREVIVRPGKRF
                     RIDDISTLNVDDGTVVSKGTEVAPGIVAGEDCVVTINFQIEEPEDEDEDIEDTSDLEY
                     AEVLLRPYQEFLVEPRKVNIEFESTDPENITVVPVTQLQVKDGDKIRHIEGAPILRTS
                     LVLQMRGYLERLRAVAEIKDGGKKDDNNLKIVVLENLTIRRESPSGMGALAKTATQTQ
                     LLVEDGQVIEKRTPVVKTQVITHTKGRVSLGNATGDKDVRRVLLITDDHETVVPTSEK
                     SSLKAGDFVKQDQPLSKSDNSPISGRVTEVDAKSVTIRDARPYLISGGTLLQVENRAL
                     TQRGDLIASLIFERQKTGDIVQGLPRVEELLEGRKPKESAIISPVDATVEIVWEDDVQ
                     RLFLGGLNGREEIQIPVGSNIIVEDKQPVKTGQALTDGPINPHDILLVSGIEAVRAYL
                     VEEVQRVYRSQGVEIADKHIEVIVRQMTKKVRVDEAGDTTLLGGELVDEKYGAECNRA
                     VEGKEGKQPAEYHPVLLGITKASLNTESFISAASFQETTRVLTEAAVEGKKDFLRGLK
                     ENVIIGRLIPAGTGFPHFKDDRVDMLDEGEQAFANKSTLRKPSAILEEIESMFGSPEI
                     SEETMENLLIDDSAMHVETIGAGSEESFDE"
BASE COUNT          734 a          810 c          824 g          466 t
ORIGIN      
        1 cggcgaagtg accagcaagc tgcccacccg cgaattccgc acccgtcacg gggacattgt
       61 tgagatgacc acccgcgact gcacgctgga agtcaaaacc accgatggca agaccgacaa
      121 gtacaacctg ccctacaaca cgctgttaat ggtcaccacc ggcgacaccg tgaaaaaaga
      181 tcaggtgttc gctacgtttg aaccaaccgc tggccgtcgg acagagaaag ccaccaagga
      241 tatcaccgcc aacatgagcg gtgaggtctg gttcgatggc ttcgacgccg atgagaaacg
      301 ggatcgtcaa ggcaacatca gccgtaccgc caaccggccg ggcaccatct gggtgctggg
      361 cggcgacgtt tacaacctgc ccaccggcgc gcgccaccta gtgcaagatg gccaggtgct
      421 gaacgtcggc gagatgatcg ctgaaaccca tgtggtgagc gagcatggcg gtgaagtccg
      481 tatccctgcg gatctcgaaa tcgaagaaca aaaagtcggc aaggaagtgg tcaaaaagat
      541 cgtcaacggt aaggaattga ccatcatcat cgccggcatc acccccacca acgctgatct
      601 ggtgcaaacc aagaaagaac agctgtggaa agtgggcgaa gacgaagaaa cctacatcgt
      661 gaaatcgccg gaaggcacca cgctggaaaa cggcagcatc atggccgagc taatcgacga
      721 gagcatgtcc gtaccgacca gcggtcagat ccgttacctg gatgtcgagg tggatgagca
      781 gcgcattctg acccaaccgg gcaaaatcct gttcctgccg gaagagattc atcaagtcag
      841 caaggacatt tcactgaaag tccccggtat cgagtccggg atggaagtga ccgccggcac
      901 ggaaatcgtg aagggcgtca ccacccagac cgacggtatc gtggaaatcg tggaagagaa
      961 cgacatcatc cgcgaagtca tcgtgcgccc cggcaagcgc ttccgtatcg acgacatcag
     1021 cacgctgaac gtggatgacg gcaccgtggt cagcaaaggc accgaagtcg cgcccggcat
     1081 cgtggctggc gaagactgcg tggtcaccat caacttccaa atcgaagagc cggaagacga
     1141 agacgaggac atcgaggaca ccagcgatct ggagtacgcc gaagtcctgc tgcggcctta
     1201 ccaggagttc ctggttgaac cgcgcaaggt aaacatcgag ttcgagtcca ccgacccgga
     1261 aaacattacc gtggtgcctg taacccagtt gcaggtgaaa gacggcgaca agatccgcca
     1321 catcgaaggc gcccccatcc tgcggaccag tctggtgctg caaatgagag gttacctgga
     1381 gcgtctgcgc gccgtggccg aaatcaagga cggcggtaaa aaagacgaca acaatctgaa
     1441 gatcgtggta ctggaaaacc tgaccatccg tcgcgaaagc ccctccggca tgggtgcgtt
     1501 ggccaaaacg gccacccaaa cccaactgct ggtggaagat ggccaagtca tcgaaaaacg
     1561 caccccggtg gtcaaaaccc aggttattac ccacaccaaa ggtcgggtga gcctgggcaa
     1621 tgcgaccggc gacaaagacg tgcgccgcgt actgctgatt accgacgatc acgaaacggt
     1681 tgtgccgacc agcgagaagt cttccctgaa agcgggcgac ttcgtgaagc aggatcagcc
     1741 gctcagcaag agcgacaact cgcccatctc tggccgagtg accgaagtgg acgccaagtc
     1801 ggtaaccatt cgcgatgccc gcccttacct gatttccggc ggtacgttgc tgcaagttga
     1861 aaaccgggcg ctcacccagc gcggcgattt gatcgcatcg ctcatctttg aacgtcagaa
     1921 aaccggggac atcgtccagg gtcttccgag ggttgaagaa ttgctggaag gccggaagcc
     1981 gaaagaatcc gccatcattt cccctgtgga tgccaccgtg gaaatagttt gggaagatga
     2041 cgtccaacgt ctgttcctgg gcggcctgaa cggtcgtgaa gagattcaga tcccggtggg
     2101 ctccaacatt atcgtggaag acaagcagcc ggtgaaaacc ggtcaggcgc tgaccgatgg
     2161 cccaatcaac ccgcacgaca tcctgctggt ttccggtatc gaagccgtac gggcctacct
     2221 ggtggaagaa gtccaacggg tataccgttc ccagggcgta gaaatcgcgg acaagcacat
     2281 cgaggttatc gttcgtcaga tgaccaagaa agtgcgcgtg gacgaagccg gtgacaccac
     2341 cctgctgggc ggcgaactgg tggacgagaa gtacggcgcc gaatgcaacc gcgccgtcga
     2401 aggcaaggaa ggcaagcagc ctgccgagta ccacccggta ttgctgggta ttaccaaggc
     2461 cagcttgaac accgagagct tcatctccgc cgcgtcgttc caggaaacca cccgcgtact
     2521 gaccgaagct gccgtggaag gcaaaaaaga cttcctgcgc ggcctgaagg agaacgtgat
     2581 tatcggtcgc ttgatcccgg ctggtaccgg tttcccgcac ttcaaggacg atcgcgtcga
     2641 tatgttggac gaaggcgaac aggcgtttgc caacaagtcc acgctgcgta agccttcagc
     2701 aatcctggaa gaaatcgaga gcatgttcgg ttcccccgaa atctccgagg aaaccatgga
     2761 aaacctgctc atcgatgatt ccgcgatgca cgtggaaacc atcggtgccg gtagcgagga
     2821 aagcttcgac gaag
//
LOCUS       DATMTI010001059         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_676516, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001059 DATMTI010000000
VERSION     DATMTI010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676516"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="VN039_16750"
     CDS             <1..1454
                     /locus_tag="VN039_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWV47648.1"
                     /translation="FDPYFHYAAFHSISGVLNEEAGKVFRFKEQEKLHEEIIDSGLAK
                     IYQSHLDISREIAQAEQTDLKTTLLDGKAFEKIIQYVRKDVPALLIIGRIGVHSDDDM
                     DVGSNTENLLRSAPCNVLVSNRKYVPPIDTQAEYTIAWTEEALRRMEKIPVFARGVAK
                     TAIHRYAIEKGHTIISNTVVDAAVGHILPKGAMEAMRALGGNLDAAGIDRDKMQADQA
                     VTKDLMGPTLSGIMTQIVEEKPKSVDASTQAYLDRMSQRYFVCDGCGYIGKGDAPVKC
                     PVCSSDGTRFKQVDKKIFEVAAKAEGELETDVAYDDVPMQWTKDAKEAIRAVPAGFQR
                     RRAKARIEKSARKLGMTTITLEYASPMIKEAAAEDYTPIFSNKGTGTSPGAEATPAAT
                     AGNGSNSHGESTSPYTWTDEAQARLDRAPEGFMRDCTRSLILKHAEKTGATVITLEVA
                     NEGIEQAKGYMAEAMKTGNLKDMIANLTGKTNT"
     gene            1539..>2715
                     /locus_tag="VN039_16755"
     CDS             1539..>2715
                     /locus_tag="VN039_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWV47649.1"
                     /translation="MGKPLPIFNGPPGTLGSLQQQVARFFTPPPKGDGPFDGRTVDDF
                     KPYLVALNLTKRCNLKCDHCYLDATTKAAGGDDELSTEECYRLIEQIAEVNKGCLLVI
                     TGGEPLVRPDILDIARYAVKLGFMVVFGTNGMLINDQLAKTLVEIGVMGVGISIDSLQ
                     AAKHDAFRGVPGAWEAAVAGIEASKRNGLQFQVHFSAQPMNYTELPEVVAWAHRLGAR
                     VLNVFFMVCTGRGEELTDITPAQYEEVLGYLIECQDNYKGMLVRARCAPHFKRLAYEK
                     DPNSPITKATGYMGGGCLAGTNYARVTPNGELTPCPYMPLSAGNVRHQSFGDLWERSD
                     VFNSFRYPQLKGKCGDCEYSDICGGCRARPYVDHGDWLDEDQWCLYTPKGGEKIKVAF
                     "
BASE COUNT          660 a          740 c          774 g          541 t
ORIGIN      
        1 catttgatcc ctatttccac tacgcagcct tccacagtat ttccggtgtc ctgaacgaag
       61 aagccggcaa agtgttccgt ttcaaggaac aggaaaagct gcacgaagaa atcatcgaca
      121 gcggcttggc caagatctat cagtcccatc tggacatttc ccgtgaaatc gcccaagccg
      181 aacagaccga cctgaagacc actctattgg acggaaaagc attcgaaaaa atcattcagt
      241 atgtccgcaa agatgtcccg gccttgctca ttatcggacg catcggcgtt catagtgatg
      301 acgatatgga tgtcggcagc aacaccgaga atctgctccg gagtgcgccc tgtaatgtgt
      361 tggtctccaa ccgtaagtat gttccaccga tcgataccca agctgaatac acgatcgcct
      421 ggaccgaaga agcgttgcgg cgtatggaaa agatcccggt cttcgcgcgg ggggtcgcca
      481 aaacggctat ccatcggtat gccattgaaa agggccatac gatcatcagt aacaccgtcg
      541 tggacgccgc agttggacat atcctaccca aaggcgccat ggaagccatg cgcgcactcg
      601 gtgggaatct ggatgcggcc ggtatcgatc gcgacaagat gcaggccgac caggccgtga
      661 cgaaggatct catgggcccg acattgagcg ggattatgac tcagattgtc gaggaaaagc
      721 cgaaatcggt cgatgcttcg acacaggcat atctggaccg catgagccaa aggtacttcg
      781 tttgtgacgg ctgcggctac atcggcaagg gagatgcgcc ggtcaaatgt ccggtgtgca
      841 gttcagatgg gacgagattc aaacaggtcg acaagaagat cttcgaagtg gccgcgaagg
      901 ctgaaggtga gctggaaacg gatgttgctt atgacgatgt gccgatgcaa tggaccaagg
      961 atgcgaaaga agcgatccgc gccgtgccgg ccggttttca gcgtaggcgt gcaaaggcca
     1021 ggatcgaaaa gagcgcgcgt aaattgggaa tgacgaccat cacgctcgaa tacgcgtcgc
     1081 cgatgatcaa ggaagccgcg gccgaagact atacccccat tttttccaac aaaggcaccg
     1141 gcacttcccc gggggcggaa gcaacaccgg cagccacggc tgggaatggt tcgaatagtc
     1201 acggcgaatc cacgtcaccc tatacctgga cggatgaagc acaagccaga ctggatcggg
     1261 ctccggaagg cttcatgcgc gattgcacca gatcgctcat tctcaagcat gccgaaaaaa
     1321 ccggagcgac cgtgatcacg ctcgaagtcg cgaatgaagg catcgaacaa gcgaaggggt
     1381 acatggcgga agctatgaag accggcaatc tcaaggacat gatcgccaac ctcaccggta
     1441 aaaccaatac atagttctga gtgctgagtg ctgagcatgg gggcacgggc gaaagccttc
     1501 tgactcagca ctcgactctc agcactcagg actcacccat gggtaaacct cttcccatct
     1561 tcaacggccc tcccggcact ttgggctctt tgcagcaaca agttgcccgg ttcttcacgc
     1621 cccctccaaa gggggacgga ccgttcgacg gtcggacggt tgacgacttc aagccctacc
     1681 tcgttgcgct gaacctgacc aagcgttgta acctcaaatg cgatcactgt tatctcgatg
     1741 cgacgaccaa agccgccggt ggagacgatg agctgagtac cgaagaatgt taccggctca
     1801 tcgagcagat tgcggaggtg aataagggct gtctcctcgt catcacgggc ggcgaaccgc
     1861 tcgtgcgtcc cgacattctt gacatcgctc gttatgcggt caaactcggc tttatggtcg
     1921 tcttcgggac aaacgggatg ctgatcaatg accaactggc caagactctt gtcgagatcg
     1981 gtgtgatggg cgtgggcatc agcattgatt ctttgcaggc cgccaagcac gacgcctttc
     2041 gcggcgtacc gggggcctgg gaagcggcgg tcgccggcat tgaagccagc aagcggaacg
     2101 gccttcaatt ccaagtacat ttcagcgctc aaccgatgaa ctatacggaa ctgcctgagg
     2161 tcgtcgcctg ggcgcaccga cttggagccc gtgtattgaa tgtgttcttt atggtttgca
     2221 ccggacgcgg cgaagagctg acggatatca cccccgctca gtatgaagag gtgcttggat
     2281 acctcattga gtgccaggat aattacaaag gcatgctggt cagagcccgt tgcgctccgc
     2341 acttcaaacg gttggcttat gagaaggatc ccaactctcc gatcaccaaa gccactggat
     2401 acatgggggg aggttgcctc gcaggcacca actacgccag ggtgacgccg aacggtgagt
     2461 tgactccttg tccctatatg ccgctgtcgg cggggaatgt ccgccaccag agcttcggcg
     2521 atctctggga gcgatcggat gtcttcaatt cattccgcta tcctcaactc aagggaaagt
     2581 gcggcgactg tgaatatagc gacatctgcg gcggttgtcg cgcccgtccc tatgttgacc
     2641 acggtgattg gcttgatgaa gatcagtggt gcctctacac cccgaaaggc ggcgagaaga
     2701 tcaaagtggc gttca
//
LOCUS       DATMTI010001060         4735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_39593, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001060 DATMTI010000000
VERSION     DATMTI010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39593"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VN039_16760"
     CDS             complement(<1..194)
                     /locus_tag="VN039_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47650.1"
                     /translation="MEIDSGHPLHFHSMDCPEHNAMIIGVSMRCEWGYLSRVLIRTEG
                     ALLLGAPRFGSSARERIVMRL"
     gene            352..3747
                     /locus_tag="VN039_16765"
     CDS             352..3747
                     /locus_tag="VN039_16765"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HWV47651.1"
                     /translation="MARQAMDEQIPTNVSRSRGRLLLLMALAIGLMAAVWWAISWSLN
                     AQTVKSALVHQVEQRTGHRLEFEDLELRLFPLPRLDLRQVKMFDRQIDAPLVSAKHVD
                     VALQIGPLLEGRAVATHVVLESPRITVRRAPSGQWTIGERKPESAADKKGNPFGFLAF
                     VRNLLIVDGGITIVDQSGSVQTDPMLLTSLQLTMTEDIPGRSVKIRVSGEMPQGTGES
                     ALFNIDGSLVLLNGEDGESPDAAALAQAEGTIRIHRLDVHYVAGAFGLHPVPSGFFPP
                     VQLLGHLRLVPRPAGYDLIVTDWRAGSSDVSLHGTADVTGLGTAEPRVSADLSSSSVS
                     LKQTLNQVSSEWIPTDLRDKLSEHAVEGFISVHDTHVEGVLGQAERLHIAGTVEIRDG
                     RFLPGGTHPAVRELSATVFYDLEQIRVTALRGNYGPVRLSDGAILITEWRQEPMVDAR
                     ISGEVRAEDLIVLLNNQGRFPQMTMGLSQYEQVTGEVGMVAHVAGRPGKGDLDIEEIS
                     VAIHHLGFRHQNVPVPFRQIEAAVRILPKEVHLDHLTGQAGYARVEAGGRVTLADEPS
                     FQDVALKITADGEDLAPWLRGAGDETLKLNVEGPVSLSASIDGHVRTPHFQGRLTLDE
                     TGFRIANVLDKAKGAPAGIRFEGRLQKDLLLSIRRCELLLPPVRLTGEGRIQLANEWE
                     FGAKIRSDALSLDKLPRGVRLGSVSAGIIEAGLKMEGRATDRASWVTSGRLRFDNGVV
                     EEQFQDPIRNLFMRLRFDGKNIDIQRLTFTVGDSDLRLSGSITNWLEAPKVKLVVQSP
                     QIDVASLKPVSSNDSSSSDSSPAVRTWWANGSLDATVLIDYLYYGQSLVSGLSCRVRF
                     EHGSLKIDRISGDTDDGHLAGRVNLNIPERGPRLISSMFGISGVPIDHLFSLLEERPR
                     ITGWMAARGGAQAELGQDSVFRSSITSRRPISIMIERGRLLYAPVISKVLVLINLPTL
                     LKGKADLTKDGIPFDRLKLVFGVEHGIINISEFLLDSPILKISGTARYDFIDDKFDGV
                     VVASPLGQYSDLLKSVPLFGKLFSGERHGFDTAIFEVKGSAKDPNVVYLPAESLMAGA
                     KGTAKLAFDLLVNAVTLPEEAFSMAEGDSPIEEDAVQEGHRGL"
     gene            complement(3811..4176)
                     /locus_tag="VN039_16770"
     CDS             complement(3811..4176)
                     /locus_tag="VN039_16770"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWV47652.1"
                     /translation="MQPFHWSSGSLMLRARSWRPFYLLWILVAGCAFGAGSGLSDSPS
                     DGAISKEVEIKLSGDQFGGLSEVLVTTEAGVVTLVGTVERAEQKARAADLARQVKGVK
                     RVKNDLEIQRTKASEAPTQ"
     gene            complement(4213..4632)
                     /locus_tag="VN039_16775"
     CDS             complement(4213..4632)
                     /locus_tag="VN039_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47653.1"
                     /translation="MNRMPLGIVHIGFACMLAAAGCASDQTARQPTSADSSVSPVTIE
                     AASVGSSDSLKACLAKISSDSSNGVRMIAEQSCQDNEKLHQGVVGTAIAKSGNRASAG
                     TQGDSHDACMARIPTDATSGQRMLAEETCARDQLTHR"
BASE COUNT          983 a         1319 c         1392 g         1041 t
ORIGIN      
        1 agccgcataa caatgcgttc gcgagcagac gagccaaacc gcggcgcgcc gagcaagagt
       61 gccccctcgg tccgtataag aactcgtgat aaatagcccc attcgcatct cattgaaacg
      121 ccaataatca tggcgttatg ctcgggacag tccatgctat gaaaatgcag aggatgaccg
      181 ctatcaattt ccaaggctcc ttcgcgcgcg aatctgttca agacgtatgt tgggtttagg
      241 gattctccct ctccaaagtc cggctgcttg tgtgccaaaa tcacctcaca tatagtgaca
      301 gaatcagaca ggcgaggccg cttgataggg cctctcgcgt aggaattgct cttggcgcgg
      361 caggcgatgg acgaacaaat tcccaccaac gtatcccggt cgcgaggtag gctccttctt
      421 cttatggcgc tcgccatcgg tctcatggcg gcggtctggt gggcaatttc atggtctctc
      481 aacgcacaga ccgtcaagag tgccttggtc caccaggtcg aacagcggac cggccatcgc
      541 ttggagttcg aggatctgga actcagattg ttcccactcc ctcgcttgga tctccgccag
      601 gtgaagatgt ttgatcggca aatcgacgcg cctctcgtgt ccgcaaaaca tgtggatgtc
      661 gcgcttcaga tcggtccttt gttggaggga cgggccgtgg cgacgcatgt ggttcttgaa
      721 agcccgcgta tcaccgtgcg gcgtgctcca tcgggacaat ggaccatagg cgagcggaag
      781 ccggagtctg ctgcagacaa gaaaggaaac ccgttcggct tcctggcctt tgtacgaaac
      841 ctcttgatcg tggacggcgg gataacgatt gtggatcaat cgggatcggt tcagacggat
      901 cccatgctct tgacctcatt gcagctgacc atgacggagg acataccggg acgttcggtg
      961 aagatccggg tgtccggaga aatgccgcaa gggaccggtg aaagcgcctt gttcaatatc
     1021 gatggctctc ttgttctgct gaacggagag gatggggaat caccggatgc cgcggccttg
     1081 gcacaagcgg agggaaccat ccgcattcat cggttggacg ttcattacgt cgccggagcg
     1141 tttggcctgc atcccgttcc gagtggtttc ttcccgccgg tccaattgct cggacatctg
     1201 cgactggtgc ctcgtcctgc cggatacgat ctcattgtga cggattggcg agccggatct
     1261 tccgatgttt ctctgcatgg aaccgctgat gtgaccggcc tcggcacagc ggagcctcgt
     1321 gtatccgcag acctgtcgtc ttcttctgtg tcgttgaaac aaactctcaa tcaggtgtcg
     1381 tcagaatgga ttcccactga cctgcgggac aaactctccg agcacgctgt tgaaggattt
     1441 atctcggtgc acgatacgca tgtggaaggc gtcttgggac aggccgaacg cctccacatt
     1501 gcggggaccg tagaaatccg ggacgggcgt tttctcccag gcggcaccca tcccgctgtc
     1561 cgggaactgt cggcaacggt gttctacgat ctcgaacaga tccgagtgac cgctctgcgg
     1621 ggaaactatg gaccggttcg tctttcagac ggggccatac tcatcactga atggagacag
     1681 gagccgatgg tcgacgcccg catctcggga gaggtccgag cggaggatct gatcgtattg
     1741 ctgaacaacc aaggaagatt cccgcagatg acgatgggtc tctctcaata tgaacaggtc
     1801 accggggaag tcgggatggt ggcgcatgtg gccggtcggc cgggtaaggg agacctggac
     1861 attgaggaga tttccgtagc gatccaccat cttggatttc gccatcaaaa cgtgccggtt
     1921 ccatttcggc agattgaagc cgccgtaaga attctcccca aggaggtcca cctggaccat
     1981 ctgaccggcc aagccggtta tgcgcgagtg gaggcggggg gcagagtgac tcttgccgac
     2041 gagccgtcat ttcaggacgt ggcgctgaag atcacggccg atggggaaga tctcgctcct
     2101 tggttgcgcg gagccggtga tgagacattg aagctgaatg ttgaaggtcc cgtctcattg
     2161 agcgcctcca tagacggtca tgttcgcact ccccacttcc aagggaggct cacgctggac
     2221 gagaccggct tccgtattgc caatgtgctc gacaaagcca aaggggcgcc ggctgggata
     2281 cggtttgaag gccggctcca gaaggacctg ctcttgtcga tcaggcgctg cgaattactc
     2341 cttccgccgg tcaggttgac cggcgaaggc cgaatccagc tggcgaatga atgggagttt
     2401 ggagcgaaaa tacggtctga cgcgctttcg ctggataagc tacctcgagg cgtgaggctc
     2461 ggctcggtca gtgctgggat catcgaggca gggctcaaaa tggagggccg agccactgac
     2521 cgcgcgtcat gggtcacgag cggccgccta cgcttcgaca atggggtggt cgaagagcag
     2581 tttcaagacc ccatcagaaa tctttttatg cggctgcgtt tcgacggcaa gaatatcgac
     2641 attcagcggc tgaccttcac cgtcggcgac agcgatctgc gcttgtcggg atccattacg
     2701 aattggttgg aggccccgaa ggtaaagttg gtcgttcaat ccccccagat cgatgtcgcc
     2761 tcgctgaagc ccgtaagctc taatgactcg tcctcttccg attcgtcacc cgccgtccga
     2821 acctggtggg ctaatggttc gctcgacgcc acggtcctga tcgattatct ctattatggg
     2881 caatctttgg tgagcggcct gtcatgccgg gtccggttcg agcacggaag tttgaagatc
     2941 gaccgaatca gcggcgatac ggatgacggt catcttgccg gacgtgtcaa tctgaatatc
     3001 ccggaacgag gtccgcggtt gatcagcagc atgttcggta tcagcggcgt gccgatcgac
     3061 cacctctttt ctcttctaga ggaacggccg cgaatcaccg gatggatggc ggccagggga
     3121 ggagcccagg cggagctcgg acaagacagt gtctttcgtt cgtcgatcac cagccgtcga
     3181 ccgatttcca tcatgatcga acgcgggcgt cttctttatg cgccggtgat ctccaaagtg
     3241 ctggttctca tcaacttgcc cacgttgctg aaaggcaagg cggatctcac caaggatggc
     3301 attccgttcg accgattgaa actcgtgttc ggcgttgagc atggcatcat caacatcagc
     3361 gaattcctgc tggatagtcc tattctgaaa atcagcggaa cggcgcgata tgatttcatc
     3421 gacgataaat tcgatggggt ggtggtggcg agtccgctcg gccaatattc cgaccttctc
     3481 aaaagcgtgc cgttgttcgg gaaactattt tccggtgagc ggcatggatt cgataccgcc
     3541 attttcgagg tcaaaggatc ggcgaaagat ccgaacgtcg tctacttgcc ggccgaatcc
     3601 ttgatggccg gagcgaaggg aacggcgaaa ttggccttcg atctgctggt taatgcggtc
     3661 accctccccg aagaagcctt ttcaatggcc gaaggtgatt cgcccataga ggaggatgcc
     3721 gtgcaggaag ggcaccgggg tctctaaacc aggagactcc ggcgctatcg aagaagcgaa
     3781 cggtaaggcg gggatgcacc cgtgccgcgg ctattgtgta ggggcctcgc ttgctttggt
     3841 ccgctgaatt tcaaggtcat ttttcacccg tttcacgcct ttgacctggc gggccagatc
     3901 cgcagccctg gctttctgtt ccgcccgttc gaccgtgccc actagcgtca cgacgcctgc
     3961 ttcggttgtg accaggacct ccgacaaccc tccgaattga tcgcctgaga gcttgatctc
     4021 cacttctttg gagatagccc cgtcgctggg ggaatcggaa agacccgagc cggcaccaaa
     4081 ggcgcagccc gcaaccagga tccacaacaa atagaatggg cgccaggatc gtgcacgaag
     4141 catcagcgac ccagaagacc aatggaatgg ctgcatcttg ttccttacgt atcgagatgc
     4201 gacttcttgt tcttaccgat gcgtcaactg atcgcgcgca caggtttctt ctgccagcat
     4261 tcgttgaccg gacgtcgcgt ctgtggggat gcgcgccatg caggcatcat gcgaatcgcc
     4321 ctgcgtcccg gcggatgcac gattgccgct tttggcaatg gccgtcccga ccacgccttg
     4381 atgaagcttt tcattgtctt gacaactttg ttcagcgatc atcctcaccc cattgctgga
     4441 atcgctggag atcttggcga ggcaggcctt cagggaatcc gacgaaccaa cggaggctgc
     4501 ctcaatcgtg accggcgaca cggatgagtc tgctgatgtc ggctgcctcg cagtctgatc
     4561 cgaggcgcaa ccagccgctg ccaacatgca agcgaagccg atgtgtacaa ttccaagcgg
     4621 catcctattc ataattagta ttcctccttt acggcggcaa tcgtgagccg gcgcggtcta
     4681 cccgtttcta tatagcggag tccccttcag ggcgcaagcc gcatcgaagg tccag
//
LOCUS       DATMTI010001061         1861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_847539, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001061 DATMTI010000000
VERSION     DATMTI010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847539"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /gene="ftsH"
                     /locus_tag="VN039_16780"
     CDS             <1..1620
                     /gene="ftsH"
                     /locus_tag="VN039_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWV47654.1"
                     /translation="LRDNTLLRSYSADYPDLVKVLREKDVRIEVKPPDESPWYMTFLF
                     TWGPFLFFLGLWFFLMRQMQMGGNQALSFVKSRARMPTEERKKVTFADVAGIDEAKEE
                     VQEIIEFLKDPRKFQKLGGRIPKGVLVVGPPGTGKTLLAKAIAGEAGVPFFSISGSDF
                     VEMFVGVGASRVRDLFEQGKKYAPCIIFIDEIDAVGRLRGAGLGGGHDEREQTLNQLL
                     VEMDGFDTTEGIILVAATNRPDVLDPALLRPGRFDRQVVVNHPDLRGRSEILKVHTKR
                     VPIAANVELEKIARGTPGFSGADLENLVNEAALWAARQNKKEVETMDFEMAKDKVMMG
                     AERKSMILSDEEKRVTAYHEAGHALMAKLLPGTDPVHKVTIVPRGRALGQTMQLPTDD
                     RHNYSKEFLYNTLAILMGGRVAEELVFKHVTTGAGNDLERATDLARKMVCEWGMSEKL
                     GPLTFGQKEGSVFLGRDFSSRRDLSDQVAIEIDMEITRFVTENYERAKFVLTEQMASL
                     KALAEALLEKEVLDARQIDQIISNSSHTVSV"
BASE COUNT          413 a          451 c          609 g          388 t
ORIGIN      
        1 ctcagagata acactctcct tcggagctac tcggcggatt atcccgacct cgttaaagtg
       61 ttgcgagaga aggatgtcag aatcgaagtc aaaccgccgg atgaaagccc ctggtacatg
      121 accttccttt tcacgtgggg accgtttctt ttcttcctcg gcctctggtt tttcctcatg
      181 cgtcaaatgc agatgggagg gaaccaagcc ttgtcttttg tgaagagccg cgcccgaatg
      241 cccaccgaag agcggaagaa ggtgacgttc gccgatgtgg ccggcattga tgaagccaaa
      301 gaggaagtgc aagagatcat cgagtttctg aaagatccgc ggaagttcca gaaactcggc
      361 gggcgcatcc ccaaaggcgt gctggtcgtg ggtccgcctg gtacaggaaa gacgttgttg
      421 gcgaaagcga ttgccggaga agccggggtg ccgttcttca gcatcagcgg gtcggatttc
      481 gtggagatgt tcgtgggggt cggggcctct cgtgtgcgcg atttgttcga gcagggaaag
      541 aaatacgctc cctgcattat cttcatcgat gaaatagacg ccgtcggccg tttgcgcggc
      601 gcgggacttg ggggcgggca tgacgaacgc gaacagacgc tcaatcaatt gcttgtcgaa
      661 atggacgggt tcgacacgac ggaaggaatc attctggtgg cggcgacgaa taggccggat
      721 gtgcttgatc cggctttgct gaggccgggc cgattcgatc gccaggtcgt ggtgaatcat
      781 ccggacctcc ggggccgatc ggagattctc aaggttcata cgaaaagggt gccgattgcg
      841 gccaatgtgg agctggagaa gattgcccga gggacgccgg gtttttccgg ggccgatcta
      901 gaaaatcttg tgaacgaagc ggcgttgtgg gcggcccgcc aaaacaaaaa ggaagtcgag
      961 accatggact tcgagatggc gaaggacaag gtcatgatgg gcgccgagcg gaagagcatg
     1021 attttgtccg acgaagagaa acgcgtgacg gcgtatcacg aggccggcca cgccttgatg
     1081 gcgaaattgt tgccgggaac ggacccggtt cataaagtga cgatcgttcc cagagggcga
     1141 gccctcggtc agacgatgca gttgccgacc gatgacaggc ataactactc gaaagagttt
     1201 ttgtacaaca cgctggcgat tcttatggga ggccgggtgg ccgaagagct ggtgttcaag
     1261 cacgtcacga ccggtgcggg caacgacctg gaacgtgcaa cagatttggc gcggaagatg
     1321 gtctgtgaat ggggcatgag tgagaagctg gggcccctga cattcggaca gaaagaggga
     1381 tcggtgtttc tggggcgcga tttttcgagt aggcgggatt tgagcgacca ggtggcgatt
     1441 gagatcgaca tggagatcac acggtttgtc accgagaact acgagcgggc caagttcgtc
     1501 ctgaccgagc agatggctag cttgaaggca ctggctgaag cgctcttgga aaaagaagtg
     1561 ctggatgcac ggcaaatcga ccagattatt tcgaactcct ctcacacggt ttctgtttag
     1621 cacgatttcg ataggggtcc gctcccattc cgggtaagcg gccccagcaa atgactggat
     1681 ggtggaatac tgcttgacgg cgagccgcaa cgacggtatc ccagtggcca tgagtcaacc
     1741 ttccaccacc gtagcctacg ggtcggtcgt gcttgcggca gtcctgtggg gtggttcgat
     1801 cgtggcacag aagatggccc tcggctcgtt ttctgccgtc gaagcgtcgg tcctgcgtga
     1861 t
//
LOCUS       DATMTI010001062         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_81829, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001062 DATMTI010000000
VERSION     DATMTI010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81829"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VN039_16785"
     CDS             <1..738
                     /locus_tag="VN039_16785"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWV47655.1"
                     /translation="VLLAEYAQELYDAGLDRVTVSVDTLRPERFRRLTGRDEFARVLE
                     GIESVGKTGFTNLKFDTVAIRGFNEDELSPLIEFAKHYQAEVRFIEYMDVGGANEWRM
                     DKVLSQDAILDALARRYGPITALPERGAAPAQRFCLPDGTIFGIIPSTTTPFCAQCDR
                     SRVTADGLWYLCLYARSGVDLRKPLRMNEHPEHMRDIIRSGWAARRDRGAEDRKALER
                     VGLRAGGLIDIDRLREDPHLEMHARGG"
     gene            847..1557
                     /locus_tag="VN039_16790"
     CDS             847..1557
                     /locus_tag="VN039_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWV47656.1"
                     /translation="MLLDPQFLSLLGVGFVLGLRHALDSDHLAAVSTVLAERPSLLAS
                     GAVGLWWGIGHTLTLLLVGSIVLAWGIRIPDEFELLAESGVAVLLIVLGGTLALKLYR
                     ERWHVHSHHHEGEPHIHFHSHQRQEDHRHRHWMVQSIRPLCIGMAHGLAGSAALMLMI
                     LSTTKEMGTGLLSILVFGLGSIIGMMVIGLTISVPVIYSRSISQRIFGTVQGLASIAS
                     LSVGIWMLVKLALSAAGG"
     gene            1647..2483
                     /gene="accD"
                     /locus_tag="VN039_16795"
     CDS             1647..2483
                     /gene="accD"
                     /locus_tag="VN039_16795"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HWV47657.1"
                     /translation="MAWFKKEKPTESAPPPRSKGSEGMWLKCNHCREIVYRKEVDRNN
                     KVCPKCEYHFPISVTERIGILIDLGTFKEWDAELEAQDPLTFQDTKSYRDRMKTHQEK
                     TGRKDALVIGEGLLNGRRVVLCVFDFSFMGGSMGSVVGEKLCRAIDRALELKLPVILV
                     TASGGARMQEGILSLMQMAKTSTAVAKLGEAKLPFISILSDPTFGGVTASVAMLGDVI
                     IAEPKALIGFAGPRVIEQTIKQQLPDQFQRAEFLLEHGMIDMIVERKRLKETVGTLVN
                     HF"
     gene            2509..3807
                     /locus_tag="VN039_16800"
     CDS             2509..3807
                     /locus_tag="VN039_16800"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HWV47658.1"
                     /translation="MSYSSVIEYLFTLQKHGIKLGLETMRILLDRIGNPHRSLRVLHI
                     GGTNGKGSTAALVASVLQRSGRRVGLYTSPHLVEFRERIRVNGCMIAEHQVEELIARL
                     RAALQDNLEPTFFEMTTALAFLYFAESEVDVAVLEVGLGGRFDATNVVEQPLASAITT
                     IGLDHQEYLGHTEEAIAFEKGGIIKPYVPIVTGRMGREAEQVLRRIARDRSAPLWQLG
                     RDFAIDGNRSERLTYRGVRRVFEDLSCGLAGRHQWDNAACALALLEAAGRAGIDTDEV
                     AVRDGLRTVSWEGRLESIDEYPTVVLDGAHNPAAAHALAGYLKDFCFSHPTSRIILVW
                     GMMRDKDRQGFIAPLLPFVSEIVLTQATLARSATVQELRATLHEWHGPVLEAALPMDA
                     MTTARGRAMPHDLICIAGSLMLLGDIKAAVRGCGLSPIRG"
     gene            3809..>4173
                     /locus_tag="VN039_16805"
     CDS             3809..>4173
                     /locus_tag="VN039_16805"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HWV47659.1"
                     /translation="MAGPLAWVRWRRVLVVAVLSLVPFPGQAADNPTGAGTSSTGSDS
                     LDITAERIDYRQDQDIYEANGSVVIQQGTIKLTSDHATIQALPGILTAVGHVHLTDPQ
                     ADVTAERMELNVNTEAGVVT"
BASE COUNT          840 a         1101 c         1292 g          940 t
ORIGIN      
        1 gtgctattgg ctgaatacgc acaagagctc tacgatgcgg ggctcgaccg ggtaacggtg
       61 agcgtagata ccctgaggcc ggagcgattt cgccgactga ccgggcggga tgaatttgca
      121 cgagttctgg aaggcattga atcagtcggg aaaacaggct tcaccaattt gaagttcgac
      181 accgtcgcga ttcgcggatt caatgaggac gaactgagcc cattgatcga gtttgctaaa
      241 cactatcagg ccgaagtccg gttcatcgaa tatatggatg ttggtggagc gaatgagtgg
      301 aggatggaca aagtcctttc tcaggacgcg atccttgatg cccttgctcg acggtacggt
      361 ccaatcacag cgcttccgga acggggggcg gctcccgccc aacgattttg cttgccggac
      421 ggcacgatct ttgggattat tccgtcaacg acgacaccgt tttgtgcgca gtgcgatcgc
      481 agtcgcgtta cggccgacgg gttgtggtat ctgtgcttgt acgcaagatc gggagtggac
      541 ctccgcaagc cgcttcgcat gaacgagcac ccggagcaca tgcgggacat cattcgatcg
      601 gggtgggctg ctcgccgcga ccgtggagcg gaggaccgca aggcgttgga gcgagttggg
      661 cttcgagccg gggggctgat cgacattgat cggttgcgag aagatcccca tctggaaatg
      721 catgcccgtg gtggatgaga gattctctga gacggagctg cgacaatgaa cgcccaccct
      781 cagtggttct gtttccgccg cctattcaaa agaagaaggt cgtccatggg ttggatttcc
      841 tagaacatgc tgctggatcc ccaatttttg tcgctgcttg gcgttggttt cgtgctgggg
      901 cttcggcatg ctctggatag cgatcatctt gccgctgtct cgacggtgct tgcggagcgg
      961 ccgtcacttc tggcatccgg agcggtggga ctctggtggg gcattgggca cacactcacg
     1021 ctcttgctgg tcgggtcgat tgtgcttgcg tgggggattc gcattccaga cgaatttgaa
     1081 ctcttggccg aatccggcgt ggcggtcctc ctgatcgttc tcggcgggac cctggctctg
     1141 aaattgtacc gtgaacgatg gcatgtgcac agccaccacc atgagggtga accgcatatc
     1201 catttccaca gccaccagcg gcaggaggac caccgacatc ggcattggat ggttcagtca
     1261 atccgcccac tctgcatcgg tatggctcac ggactggctg gatcagcggc attgatgttg
     1321 atgattcttt caacgacgaa ggagatgggg accgggcttc tgtccatcct cgtcttcggt
     1381 ctcggttcaa ttatcggtat gatggtcatc gggctgacga tcagtgtgcc ggtcatctat
     1441 tcgcgttcga tcagtcagcg gatttttggg acggtccaag gtctggccag catcgctagc
     1501 ctctcagtgg gaatatggat gttggtgaag ttggcgttgt cggctgcggg gggatgagac
     1561 gatctcgttc tgtggatatc gacatgcata ccctcacttc ggcactgctt ctaagactgg
     1621 agtgtaagac ccgataggag aacgcgatgg catggttcaa gaaagagaag ccgacagaat
     1681 ctgctccccc gcctcgctcg aaagggagcg aggggatgtg gctcaagtgc aaccattgcc
     1741 gggaaatcgt ctatcggaag gaagtcgacc ggaacaataa agtgtgtccg aagtgcgagt
     1801 atcacttccc aatctcggtc acggagcgaa tcgggatact gatcgatctt ggtacgttca
     1861 aagaatggga tgctgaactg gaagcccaag atcccttgac cttccaagac accaagtcct
     1921 atcgagaccg catgaagacc caccaagaaa agaccggacg gaaggatgcc ctcgtcatcg
     1981 gcgaaggcct gctcaacggg cgccgtgtgg tgctctgtgt cttcgatttc agctttatgg
     2041 gcggtagtat gggatctgtg gtcggggaaa agctctgtcg tgcgatcgat cgggcgttgg
     2101 aactgaaact gccggtcatc ttagtcaccg cttccggggg cgctcggatg caggaaggca
     2161 ttctctcgtt gatgcagatg gcaaagacgt cgaccgcggt cgcgaagttg ggcgaggcta
     2221 aactgccgtt catctcgatc ctctccgatc ccacgttcgg cggcgtcaca gccagcgtgg
     2281 cgatgttggg ggatgtcatc attgccgagc ccaaagcgtt gatcgggttt gccggacctc
     2341 gtgtcatcga acaaaccatc aagcaacagt tgccggatca gttccaacgg gcggaatttc
     2401 ttctcgaaca cggcatgatc gatatgatcg tcgaacgtaa gcgcctcaag gagacggtcg
     2461 gcaccctcgt gaatcacttt tagccttctg cgtcctcccg gcttgttgat gagctactcc
     2521 tccgtcattg agtatctctt cacgcttcag aagcacggca tcaagctggg cctggagacg
     2581 atgcggattc tgttggaccg gatcggcaat ccccatcgct cgcttcgcgt gctgcatatc
     2641 ggagggacca acggcaaggg ttcgacggcg gcactggtcg cgtcggtcct tcagcgttca
     2701 ggccggcgtg tcgggctgta tacgtctccc cacttggtcg aattccgaga gcggattcgt
     2761 gtcaacgggt gcatgattgc ggagcatcaa gtcgaggagt tgatcgcgcg cttacgagcc
     2821 gctctacaag ataacctgga gccgaccttt tttgagatga cgacggcgct ggcgttcctc
     2881 tatttcgcag agtcggaagt cgacgtcgcc gtactggaag ttgggttggg cgggcggttc
     2941 gacgcgacca acgtcgtgga gcaaccgctg gccagtgcaa ttacgacgat cggcttggat
     3001 catcaggagt atttggggca cacggaggaa gcgatcgcgt ttgaaaaagg gggaatcatc
     3061 aaaccctatg tgccgatcgt gaccggacgg atggggcggg aagccgaaca ggttctacgc
     3121 cgtattgcgc gggatcggtc agcccccttg tggcagcttg gccgggactt tgcgatcgac
     3181 ggaaatcgtt ccgaacgact gacgtatcgt ggagtgaggc gcgtgtttga ggatctttct
     3241 tgcggcctgg caggacgtca tcagtgggac aatgccgctt gcgcgttggc actccttgaa
     3301 gcggcgggtc gagcaggaat cgacacggat gaggtagccg ttcgtgatgg cctgcgtacg
     3361 gtctcatggg aaggtcgttt ggagtcgatt gacgagtatc ccacggtcgt gctcgacgga
     3421 gcccacaacc ctgccgccgc gcatgcgctc gcggggtatc tcaaggactt ttgcttcagt
     3481 catcccacct ctcggattat cctcgtatgg ggcatgatgc gggataaaga tcgacaggga
     3541 tttatcgctc cactgttacc gtttgtgtcg gaaatcgtgc tgacgcaggc gactcttgca
     3601 cggtctgcta cggtccaaga actccgtgca actctgcacg aatggcatgg gccggtgctt
     3661 gaggccgctc ttcctatgga cgcaatgacg accgccagag gacgagccat gccgcacgat
     3721 ctcatctgta ttgccggatc attgatgctc ctaggggata tcaaagcggc agtgcgcggc
     3781 tgtgggctgt caccgattcg tggctagcat ggcgggtcct ctcgcgtggg tccgctggcg
     3841 gcgcgttctc gtcgtcgccg ttctctctct cgttccattt cctggacaag cggcagacaa
     3901 cccaacgggt gctggaacat cgtccactgg atctgactcc ctcgacatca ccgcagagcg
     3961 tatcgactat cgacaagatc aggacattta tgaagcaaac ggatcggtcg tcattcagca
     4021 gggaacgatt aagctgacgt ccgaccacgc caccattcaa gccttgccgg ggattctcac
     4081 tgccgtcggc catgtccatt taacggatcc gcaggctgat gtgacggctg agcggatgga
     4141 actcaatgtc aatactgagg ccggcgttgt gac
//
LOCUS       DATMTI010001063         1788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_761207, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001063 DATMTI010000000
VERSION     DATMTI010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761207"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1360
                     /locus_tag="VN039_16810"
     CDS             185..1360
                     /locus_tag="VN039_16810"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV47660.1"
                     /translation="MSLEEAIGLFLRQNLDLLIVKYGIAFSKGQQITARLFPNPIASM
                     GLQSSPVQGRTLGNSGEIAPQIQQLFELAGKRGYRIESAQYGLQSAEAAFEDAVRQLS
                     FAVKDAYYRVQLARRRLVLAEENRNRFSRILEVNTIRFKKGYIAEVDLIRIRLQFIDF
                     HSQVIQAIQEAQSARSDLRQLLQVSPASELDLTTELEYKRVDPDIVKLRAVAVDGRPD
                     VRAKRSMLSQREAELKLAKAYRTPDITVGAGYGTQGSHGPDNPNQMMFSLGMPLPLFN
                     RNQGGIMQAEAAMQAAGADLSKTLNQVENQVDVAYRNLIESRRLVEAFLGGVLDDARS
                     TIAIVERAYERGGVTLLDLLDAARTSRTIQQSYIEALFNYQRTVIQLESAVGQEIAS"
     gene            1401..>1788
                     /locus_tag="VN039_16815"
     CDS             1401..>1788
                     /locus_tag="VN039_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV47661.1"
                     /translation="MKAFLGCSGNRFPRLSSVSALIWTVLFLSTCGPSDQPPKLDAAT
                     HQPAEKGTLSHVETMIVEFSSSRQSVALSGKIAYGEDRYSRISSPLQGRVVEVRAHLG
                     GKVKAGDILLIVDSPDIAQAYSEYVKE"
BASE COUNT          401 a          466 c          525 g          396 t
ORIGIN      
        1 tacgccttcg caccgtatat gacgtgatga gagaatggtt cgacgactac gtattgcctc
       61 cctctgcctc gccgtctcaa tcggcagttc cgttggtggt gagtctctgt cagccgggca
      121 gttgatgagc gtcgcttcgg atgaggcacc accggttgcc ataccctcag caccaactct
      181 acgcatgagc ctcgaagagg cgatcgggct ctttttacgt cagaacctcg atctgctaat
      241 cgtgaaatac ggaattgcgt ttagcaaggg acagcagatt accgccagac tgtttccgaa
      301 tcccattgcc tcaatgggac tgcagagctc accggtccag ggccgcacgc tagggaacag
      361 cggagagatc gctcctcaga tccagcagtt gttcgagttg gcggggaaac gaggctatcg
      421 gatcgagagc gcgcaatacg gactacagtc tgcggaggct gcctttgaag acgccgttcg
      481 gcagctgagt ttcgccgtca aagatgccta ctaccgtgtt caattagcac ggagacgact
      541 ggttttggcg gaggaaaata ggaatcgttt ctcacggatc ctcgaagtca acacgattcg
      601 attcaagaaa ggctatattg ccgaggtcga tttgatccgt atccggttgc agtttatcga
      661 tttccactcc caggtgattc aggctattca agaagcgcag tcggcccgat cggatcttcg
      721 gcaattgtta caagtctcgc cggcgagcga gttggatctc accaccgaac tggagtacaa
      781 gcgcgtggat cctgacatcg tcaagctccg tgccgttgcc gtcgacggac gacctgatgt
      841 tcgtgcgaag cggtctatgc tgtcacagcg cgaggccgaa ctgaaactgg ccaaagcgta
      901 ccggactccg gatatcaccg tcggcgcggg ctatggtacc caaggctcgc atggccccga
      961 caatcctaat cagatgatgt tcagtctcgg catgccgctg ccattgttca atcgcaacca
     1021 aggcggtatc atgcaggcgg aggccgcgat gcaggctgca ggagcggacc tgagcaagac
     1081 attgaatcaa gtcgagaatc aagtggatgt cgcctatcgc aacttgatcg aaagtcgtcg
     1141 attggtcgag gcgttcttgg ggggggtgct tgatgatgcg cggtcgacga ttgcgatcgt
     1201 ggagcgagct tatgagcggg gtggggtcac gttgcttgat ttgctcgatg cggccaggac
     1261 gtcgaggacg attcagcaaa gttatattga agccttgttc aattaccaac ggactgtgat
     1321 tcaactggag agcgctgtcg gacaggaaat cgcatcatga ggcagccggc aagaatcggg
     1381 ttgaggtctc agacccacag atgaaagcct ttctagggtg ttccggcaac cgtttccccc
     1441 gactttcctc agtctctgct ttaatctgga ccgtactctt tctctccaca tgcggtccaa
     1501 gcgatcagcc gccgaaactc gatgcggcga cacaccaacc tgccgagaag gggacgctgt
     1561 cacatgttga gacgatgatc gtcgagttca gttcatctcg acaaagcgta gccctgtcgg
     1621 gcaagatcgc ctacggcgag gaccggtatt cgcggatctc ttcaccgcta caagggcgag
     1681 tcgtggaggt gcgagcgcat cttgggggga aggtgaaagc cggagatatc ttactcatcg
     1741 tggatagtcc ggatatcgcg caggcatatt cggaatatgt aaaggaag
//
LOCUS       DATMTI010001064         6835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_676621, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001064 DATMTI010000000
VERSION     DATMTI010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676621"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VN039_16820"
     CDS             complement(<1..229)
                     /locus_tag="VN039_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47662.1"
                     /translation="MTNSIGKPAIPPWLYKLFTGHQYPYVRRLAKFGQTVKPGEDRPE
                     PTKDMIETKFWEVYPRCRVKVLQEVKEGMIVV"
     gene            complement(242..3229)
                     /locus_tag="VN039_16825"
     CDS             complement(242..3229)
                     /locus_tag="VN039_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWV47663.1"
                     /translation="MSNRQSLLQRLVPELSPAVAETVVAALPQATGKSDAEAQVRTLL
                     DELQELSEKAAGAAVMALPELDRRAGLSHIILWLDLGVALAQSSGASALKYFKDSPLV
                     LGLIEPFDARTEVLTIGLEIAEQDANVALEYIRQAPQILSDVPAPQLRPWLDIGIELT
                     EVNVVVGLEFIRQISKLAPVLSLESARSWATVGMKLIVPNSLGRPDYVATMEFLRTSP
                     SILGNIEDPPVREKLVSLCLLLAEHSPESSITWLAESPALLRTVPSMEWRIRLLQYGA
                     LLAETHADVTLDYLRRAPELVGLIGNGPEAFARFENWFKAGMEVAAYSPEGARAYFSV
                     ESRKALVSVEQALSGAPFRQVARQVKLFVQGLCGTDVTVTALPDSVTSSARATVSHDG
                     RSISLPALLRRYPTAAENERLYLVMAAHEAGHLEFGTYRLRLESLTDLIETVRRRYGR
                     SNEAGPDTLAALFQMYPSPGLVRDLWIVLEDARIEFLLQTEYPGLRRDLARFAAESIV
                     PRDPAQGVTPKELIVDGLLRLSTGETEDSAVPKAVREEVSILWSMCRSILKSTATAEE
                     TVRVVDQVYVRLEELLVARDDMMCPERRAEPEDAETGQTKPEQPGDQYRAVTDLAYRG
                     SMNPEFIAWSQEQFDQQPEPPTQSDGRAKGNVRIGEQDPGSRDMLSDGRSLPSIVEEC
                     LTLEVETPPIRELAAHDGRAILYAEWDYRIADYRMNWCRVVERAAEAGSDDFVTETLA
                     SHRSTVKSLRRFFEGLRPPAFRRIAGQPDGEEVDLDAVVRRTTEQRAGMEGDDRLYIR
                     REKRERDVAVAFLIDISGSTSRDLGTGRRVIDIEKEGLVLLCEALDAVGDQYGLYAYS
                     GQGRAKIEFLTIKDFDDRLGAATAHRLGGLAPRHQNRDGAAIRHATAKLLTREAKNRV
                     LILLSDGRPLDDQYKDDYSLEDTKAALREARQRGVETFCITIDREAENYLRRMYADAQ
                     YCVIDRVEALPTKLPGIYRRLTA"
     gene            3336..3770
                     /locus_tag="VN039_16830"
     CDS             3336..3770
                     /locus_tag="VN039_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HWV47664.1"
                     /translation="MAEPTQESLDKIRKFVQGFAEKSGTAMHPNTAVTEAVVKGLASH
                     IDELGKPLCPCNFYKDKESEAKLRRWICACDEMQIYKYCHCLLFVREDGMPITEYLPD
                     GHEGREVYGLVTDPTPDKGRALKHKAVPSPTEPDESAAASST"
     gene            3767..4384
                     /locus_tag="VN039_16835"
     CDS             3767..4384
                     /locus_tag="VN039_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47665.1"
                     /translation="MIWRDRQLRCWLAGLLLISGFPVTAAQAVVSGSSNPPSFNEKEF
                     FAYRAKGRGSAAGQVFLRAPSGKAVTQAGVSIYLIPMVPYTRYWFDKHVRDYSCSLKG
                     GASSSEPLMTQGSPVECLREVMAQLLADKRLIPYLRATRANPSGHFWFTKIPAGRYYV
                     VSLLEGSGGAHQDERAVGIAWLIIDLEADEKATNLVVTDCKSSLC"
     gene            4436..5119
                     /locus_tag="VN039_16840"
     CDS             4436..5119
                     /locus_tag="VN039_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV47666.1"
                     /translation="MRILLVEDDADLAQFIRKGLKEEYYAVDVAVDGEAGLALALDTP
                     YDLVILDVMLPKLDGLTLCRRIRDKGITTPVLLMTARNTVETKVSGFDTGADQFLPKP
                     FAFSELLARVRALLRRGSSQQIAHLQAADLRLDPASHRVWRAEHEIFLTNKEYALLEF
                     LLRNKNRVLTRTAIIEHVWDISYDPMTNIVDAHIRALRAKIDRDFSPPLIATVRGAGY
                     MLEEPDSPA"
     gene            5116..6552
                     /locus_tag="VN039_16845"
     CDS             5116..6552
                     /locus_tag="VN039_16845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV47667.1"
                     /translation="MKSLRSLISWSAFVLMTGLLAFSALVYAASEILLQRLVDGRLLV
                     LAESLAEIVERYPDFLKTNAKDTAPTNELTQSTADQHVLPDITHALRVFSTNGSLLWR
                     SPHATVQGPLPPHVLEQVRLKNILFATLDAPDGTPARHLFLTFVGKDHVRYILQAETS
                     LLHYQETLYGLVFLLTLGSGATLILAWVGSLWLSKKVLAPIEALCTGAETMSEADLEK
                     RLAVDSPYREFRRLTQAVNSVLDQFQRSSEVQRNFCEIAAHEMKTPLTILQGNLEVAL
                     MKARTVEEYHEVLLSNLQQVDRLIALTRPLLTLAKFTSSKPPVNLVPLVLEPLIQEIV
                     DELTLLADDRQIALTFESHPVPSVLGDAQWLKQALINLLDNALRYTPSGGAVSIRLQA
                     IGQEVSVSVEDTGHGIEPEHIPHLFERFYRTDWARAKDAAGTGLGLPIVKEIMDAHGG
                     SISVTSEVNKGSIFTLRLPALSQQTALA"
BASE COUNT         1445 a         2001 c         1840 g         1549 t
ORIGIN      
        1 agaccacgat catcccttct ttgacttcct gcaatacttt tactcggcaa cgaggataga
       61 cttcccagaa ctttgtttcg atcatgtcct tcgtcggctc cggacggtct tcaccgggtt
      121 tcaccgtctg tccgaacttg gctaaccggc ggacgtaggg atactgatgt cctgtgaaga
      181 gcttgtacaa ccacggagga atcgccggct ttccgatcga gttcgtcatg ttcttgagtt
      241 cttatgcggt taatcgcctg taaattccag gcagcttcgt cggaagggct tcgacgcggt
      301 ctatgacgca gtattgtgca tcggcgtaca tgcggcgtag ataattttca gcttcccgat
      361 cgatcgtgat acaaaaggtc tcgactcccc gttgtcttgc ctcgcggagc gccgctttcg
      421 tatcttccaa cgagtagtcg tccttgtact ggtcgtccaa tggtcttccg tcgctcaaca
      481 gtatgaggac acggttcttg gcttccctcg tcagcagctt cgccgttgcg tgacgaatag
      541 ccgcaccgtc gcgattctga tgacgaggcg ccaagccacc aagccgatgc gctgtcgccg
      601 ccccaagtcg atcgtcaaag tctttgatcg tgagaaactc tatcttcgct cgtccttggc
      661 ccgagtaagc atagagtcca tactgatcac cgacagcatc cagtgcttca cagagcaata
      721 ccaaaccttc cttctcaatg tcgatcaccc gtcgaccggt tccaaggtct cggcttgttg
      781 atccactgat atcgatgagg aaggccaccg ccacgtcgcg ctctcgtttt tcccgccgga
      841 tgtatagccg atcatcgcct tccatgcctg cccgctgttc cgttgttcgc ctgacaacgg
      901 catcgagatc cacctcttct ccatcgggtt gccccgcgat tcgtcggaaa gcgggagggc
      961 gcaagccttc aaaaaatctg cgcagtgatt tgacagtact ccggtgggag gccaacgtct
     1021 ccgtgacgaa gtcgtcggat ccggcctccg ccgctcgttc caccacgcga caccaattca
     1081 tccggtaatc cgcaatccga tagtcccact cggcatagag gatggcgcgc ccgtcatgag
     1141 ctgcgagctc ccgaatcggt ggtgtttcca cctcaagcgt gaggcactct tccacaatgg
     1201 acggcaatga acgcccatcg ctcaacatat ccctgcttcc gggatcttgt tcgccgatcc
     1261 ggacattccc tttcgctcga ccgtccgatt gtgtcggagg ctccggttgc tggtcgaatt
     1321 gttcctgact ccatgcgatg aactccggat tcattgagcc acgataggcc aaatcggtca
     1381 ccgcgcggta ttggtcaccc ggttgctccg gtttcgtctg tcccgtttcc gcatcctccg
     1441 gctctgctcg tcgctccggg cacatcatat cgtcgcgagc caccaacagt tcttccaagc
     1501 gaacatacac ctgatcgacc acgcgaaccg tttcttctgc cgtggccgta gacttgagaa
     1561 tggaacggca catgctccag aggatagaga cttcttccct gacggccttg ggcaccgccg
     1621 aatcctccgt ttcaccggtg gagagtctca aaagaccatc gacgatcagt tccttgggcg
     1681 tcactccttg agccgggtcg cgcggaacga tggattcagc agcgaatcgc gcaaggtcgc
     1741 gccggagtcc cggatactcg gtttgcagca agaattcgat ccgcgcatct tccagtacga
     1801 tccagaggtc ccgcaccagc ccggggctcg gatacatttg aaaaagggcg gccagtgtat
     1861 ccggtccagc ttcattggat cgaccatacc ggcgccgtac ggtttcaatc aaatcggtca
     1921 gcgactcaag ccggaggcga taggtaccga actccaaatg tccggcttca tgtgccgcca
     1981 ttacgagata gagacgttcg ttttccgcgg ctgtcggata tcgacggagc agcgccggca
     2041 aggaaatgct tcgaccgtcg tgactgaccg tcgcgcgggc cgaagaagtc acagaatcag
     2101 gaagcgccgt caccgtcacg tcggttccac agagtccctg gacgaacagt ttcacttgtc
     2161 gcgcgacctg ccgaaacggc gccccgctca aggcttgttc gaccgaaacc agcgcttttc
     2221 ttgactccac tgaaaaatag gcacgcgctc cctctgggct gtaggccgct acttccatcc
     2281 ccgccttaaa ccaattctcg aatcgcgcga aggcttcggg tccatttcct atgagtccga
     2341 cgagttcggg ggcgcgacgc aggtaatcga gtgtcacgtc agcgtgtgtc tcggcaagca
     2401 gagcgccgta ctgcaacagc cggatccgcc attccatcga cggcaccgtt cttaagaggg
     2461 cgggtgactc tgcgagccac gttatgctgg actcggggga atgttcagcc aacaagaggc
     2521 acagggatac gagcttttcc cgaacgggag ggtcttcgat atttccaagg atcgacgggc
     2581 tggtcctgag aaactccatg gtggccacat agtccggcct cccaagacta ttcggcacga
     2641 tcaatttcat gcccaccgtg gcccagctac gcgcgctctc caaagacaac acgggggcca
     2701 gcttggaaat ttgtcggatg aactcaagcc cgacgacgac attgacttcg gtcagttcaa
     2761 tgccgatatc gagccagggt cgtaattgcg gggcaggaac atcggacaga atctgtgggg
     2821 cctgtcggat gtattcgagc gccacgttgg catcttgctc agcgatttcc agaccgatcg
     2881 ttaagacttc cgtgcgcgca tcgaatggtt cgatcagccc aaggaccaag ggactatctt
     2941 tgaaatattt gagcgcggat gccccggagg actgagccag tgccactcca agatcgagcc
     3001 acaaaatgat atgcgagagg ccggctcgcc ggtcaagctc aggcaatgcc atcaccgctg
     3061 cgcctgctgc tttttcagac aactcttgga gttcatccag caaggtccgt acctgtgcct
     3121 cggcatccga tttaccggtg gcctgtggca aagcagccac tacggtctct gcgacggccg
     3181 gactcaattc gggaaccagc cgctgtagca agctctgtcg attagacatg tccatcatgg
     3241 cttattacga gaaggagctg aatggacgga ttgtaaagca gcgaattatc cttgtaaact
     3301 atcgatctgg ccgtcgtcgc tggaggagga agtccatggc cgaaccgaca caggaaagcc
     3361 tcgataagat ccgaaagttc gtacagggtt ttgccgagaa aagcggaacc gcgatgcatc
     3421 ccaacactgc tgtgacggag gcggtggtca aggggttagc gtcccatatc gacgaactcg
     3481 ggaaaccact ctgcccttgc aatttttaca aggacaaaga atccgaagcc aagctccgac
     3541 ggtggatctg cgcctgcgac gaaatgcaga tctacaaata ctgtcattgt ctcctttttg
     3601 ttcgggaaga tgggatgccg attacggaat atttgccgga tggccacgaa ggtcgagaag
     3661 tgtatggtct ggtcaccgat ccgacccccg acaaaggccg cgccctcaaa cacaaggccg
     3721 tcccgtctcc aacagagccc gacgaatccg ccgcggcttc ttcgacatga tatggcgtga
     3781 caggcaactc cggtgttggc ttgcagggtt gttgctgatc agcggcttcc ccgtgacggc
     3841 ggcacaggcg gtggtgtcgg gctcatcgaa cccaccgtcc ttcaatgaga aagaattctt
     3901 tgcttacaga gcgaaaggtc ggggaagtgc ggccggacag gtgttcttgc gcgcaccctc
     3961 tggaaaagcc gtgactcagg cgggggtctc gatctacttg attccaatgg ttccatatac
     4021 ccgctattgg ttcgataaac atgtgcgaga ttactcctgc tctttaaagg gcggggcatc
     4081 gtcctcagag ccccttatga ctcaggggtc gcctgtggaa tgccttcgtg aggtcatggc
     4141 ccaactgctg gccgataaac gtcttattcc ctatcttcgt gcgacgagag ccaatccaag
     4201 cggtcatttt tggttcacga aaattcccgc cggccgttat tatgtcgtca gcctccttga
     4261 ggggagcggt ggagctcatc aggatgagcg ggcggtgggg atcgcctggc tgatcatcga
     4321 ccttgaagcc gacgaaaagg cgactaacct ggtggtcacg gattgcaaga gcagcctatg
     4381 ttgacccacc gcgtccttcg cttccggcct gcgtggtcca gtcatgaggt agatcatgag
     4441 aattctgctc gttgaagacg atgcggatct tgctcaattc atcagaaaag ggttgaaaga
     4501 agagtactat gcggtggacg ttgccgtcga cggcgaagca ggcttggcgt tggcgttgga
     4561 caccccctac gatctcgtga ttctcgacgt catgctgccg aagcttgacg gtctgaccct
     4621 ctgtcgccgc attcgagaca agggtatcac gactccggtg ctgctcatga cagcgcgcaa
     4681 cacggtggaa acgaaggtct ccggttttga cacgggggcg gatcaatttc tgccgaagcc
     4741 gttcgccttc tcggaattgc tcgcccgcgt tcgggctctc ttgcgccgag gcagttccca
     4801 gcaaattgct catcttcaag cggccgacct tcggttagac cccgcttctc accgtgtctg
     4861 gcgcgccgag catgaaatct tcctgacgaa taaggaatat gccctattgg agtttctctt
     4921 acggaataag aatcgggtgc tcacgcgaac cgcgattatt gaacatgtgt gggacatcag
     4981 ctatgacccc atgacgaaca tcgtcgacgc ccatattcgg gctctacgcg cgaaaatcga
     5041 tcgggatttt tcgccgccct tgatcgccac cgtgcgcggc gccgggtata tgctcgaaga
     5101 accggactca cccgcatgaa atctctgcgc agtttgatca gttggtccgc cttcgtcttg
     5161 atgaccggtc ttctcgcgtt ttctgcgctc gtgtatgccg ccagcgagat cttgttacaa
     5221 cggttagtgg atggacgact attggtcttg gcggagtccc ttgccgagat tgtagaacgg
     5281 tatcccgact tcctgaaaac caacgctaag gacactgcgc cgaccaatga attgacccaa
     5341 agcaccgcgg accagcacgt actgcccgac attacgcacg ctcttcgcgt cttttcaacc
     5401 aacggctcgc tcttgtggag aagtcctcac gcaaccgtgc aggggccgct tccccctcat
     5461 gtgttggaac aagtccgcct taaaaatatc ctattcgcga cgcttgatgc gcctgatggg
     5521 acgccggcgc ggcatttgtt cctcaccttt gttgggaaag accatgtgcg gtacatcttg
     5581 caggcggaaa cgtcattgct ccattaccag gaaactctgt atgggcttgt gtttctgctc
     5641 acccttggat ccggggccac ccttatcctg gcctgggtcg gcagcctctg gttgtcaaag
     5701 aaagtgctgg cccccatcga ggcgttgtgt acgggcgccg aaaccatgtc ggaagccgac
     5761 cttgagaaac gattggcggt ggattcaccc tatcgagaat ttcgtcgact cacccaggcg
     5821 gtcaactccg tgttggatca gttccaacga agtagcgagg ttcaacggaa cttctgcgaa
     5881 atcgctgccc acgagatgaa gacccctctg accattctgc aaggtaacct agaggtcgca
     5941 ctcatgaaag cgaggaccgt cgaagaatat catgaggtgc tgctcagcaa tcttcagcaa
     6001 gtggatcggc tcattgcttt gacccgccca ttgttgactt tggcgaaatt taccagtagc
     6061 aaacctccgg tcaatctcgt gcctcttgtc ttggaaccgc ttattcaaga gatcgtggat
     6121 gaactgaccc tgttggcgga tgaccgccaa attgctctga cgtttgagtc ccacccggtt
     6181 ccgtccgtcc tcggcgatgc gcaatggctt aagcaagcgc tgatcaatct gctcgacaat
     6241 gcgttgcgct acaccccatc cggtggagcc gtctccatcc gcctgcaggc cataggccaa
     6301 gaggtgtcgg tgtcggtcga ggacacaggc cacggcatcg agccggagca tattcctcat
     6361 ctgttcgagc gattctatcg gaccgattgg gcccgagcga aggatgcagc gggtaccggg
     6421 ctgggactac ccatcgtgaa agaaatcatg gatgcccacg gcgggagcat ttccgtcaca
     6481 agcgaagtca acaaaggctc tatcttcacg ctgcgtttac cggccctctc tcagcaaacg
     6541 gctcttgcct gaagcacttt ggcctcgtcc accttcgttc tgacgccatt ctaacccgtc
     6601 tcctacacac cgtttgcctg ccaagtgctt gacgctcttg ccaaagaccc ttctttacaa
     6661 tatttgccgt aggatttctc tctttatgac ggcaatcgca ctattcaccc ccacaacatt
     6721 cgcacgaccg taaccgacac gaaatgatct cccgataggc cggcatcatg ccaatcggcg
     6781 cgacggccac ggttgctcaa atactgccat atggaagaga ggtgggaagg atgac
//
LOCUS       DATMTI010001065         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_718377, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001065 DATMTI010000000
VERSION     DATMTI010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718377"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VN039_16850"
     CDS             complement(<1..357)
                     /locus_tag="VN039_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47668.1"
                     /translation="MKYGLNIRPHGALDFVSVGAMVHRLDPGIVPFRKASQCQIHVSG
                     GEFNVAANLSDCFAMRTGLATAMVDYPIGDLIAERVRAMGVKPFYKHFKHNGVDGPNM
                     ATVYSDRGQGVRAPVVF"
     gene            complement(415..1392)
                     /locus_tag="VN039_16855"
     CDS             complement(415..1392)
                     /locus_tag="VN039_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47669.1"
                     /translation="MPKRFANAWIWTLVLGPKFSKRERTAVNLHRDYSINQIEYCRNF
                     IFRRNFPIHKIFERSCEMGLFRLAADKVAHIFGVRVTKRMRGQLYSLLDRLDHGHHVL
                     RLYCKGLVGRMYEKFSTFLRLEICVNRLKDLGLKKGLDNLETVRQRLISATDRFAGFE
                     AESLNVHVDFPLFQRLALPVLAGKSKIPGIKIQDTRMIRLIEILLNAGTQLNGWRTAQ
                     IHQAITTTFELPPGSYTLTQLRYDLRKLKAHGLLERDGRRYCYRLTGKGTKVALMMTL
                     FHKRVCGPLANSLFHHRPNDNFKPNGKIETAYHKADKAIQRVVDLLAAA"
     gene            complement(2191..>2342)
                     /locus_tag="VN039_16860"
     CDS             complement(2191..>2342)
                     /locus_tag="VN039_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47670.1"
                     /translation="ERLPANLLQAQRDFFGAHTYERVDRPRGEFFHTNWTGRGGRVSS
                     GSYNA"
BASE COUNT          504 a          621 c          668 g          549 t
ORIGIN      
        1 gaaaacgacc ggcgcgcgaa cgccctgccc gcgatcgcta tacacggtcg ccatattcgg
       61 cccgtcgact ccgttgtgct tgaaatgctt gtaaaacggc tttacaccca tggcgcgcac
      121 acgttcggcg atcaggtcgc cgattggata gtccaccatg gcggtcgcaa gcccggtacg
      181 catcgcgaaa cagtccgaaa gattcgcggc tacgttgaat tcgccgccgc tcacatggat
      241 ctggcattgc gatgccttcc gaaagggaac gatgccggga tcgaggcgat gtaccatggc
      301 gcctaccgaa acaaaatcca gagcgccgtg cgggcggatg ttcaaaccgt atttcatatt
      361 gcctcccatt ttagcttctt aaccgaagga tctgagaatt tatctctgac attctcaagc
      421 tgccgcgagc agatcgacga cgcgttgaat ggctttgtcg gctttgtgat aagcggtttc
      481 aattttgccg ttgggcttga aattgtcgtt ggggcggtga tggaacagag aattggcgag
      541 tgggccgcac acgcgtttgt ggaacagggt catcatcaga gcgaccttcg ttccttttcc
      601 ggtgaggcga tagcagtatc gccgcccgtc tcgttccagc agtccatggg ctttcaactt
      661 gcggagatcg tagcggagtt gagtgagggt gtatgagcct gggggcaatt cgaaagtggt
      721 ggtgatcgcc tggtgaatct gagcggtccg ccatccgttg agctgtgttc cggcgttcaa
      781 cagtatttcg atcaaccgga tcattcgggt gtcctgtatt ttgattccag gaattttgga
      841 cttgccggca aggacgggga gggcgagccg ctgaaacagc ggaaagtcga cgtgaacgtt
      901 cagtgattcc gcctcgaaac cggcgaagcg gtcggtggcg gagatgaggc gctgccgtac
      961 cgtttctagg ttgtccagcc ctttcttgag gcccaggtct ttgagacgat tgacgcagat
     1021 ctccagccgc aggaatgtcg agaacttttc gtacatgcgt cccacgagac ctttgcagta
     1081 aagccgcagc acgtgatggc catggtccag cctgtccagg aggctgtaga gttggccacg
     1141 cattcttttg gtcacgcgaa caccaaaaat gtgagccact ttgtcggccg ccaagcggaa
     1201 caaccccatc tcgcatgagc gttcgaagat cttgtgaatc ggaaaattgc gtcggaagat
     1261 gaaattccgg cagtattcga tctggttgat ggagtagtcc cggtgcaggt tgacagcagt
     1321 gcgctctctt ttggagaact tcgggccaag caccagcgtc cagatccagg cgtttgcgaa
     1381 tcgttttggc atcgagcttg tcagctgcgg cctgcaatgc cttcacgtcg gcggtccaca
     1441 aaaaggcatt gtcgtctttc cggaaccgaa cgccttgccg cttcagttca ccctccatga
     1501 aatggtgccc attgatgtag tacgtggtct gaaagggcag gaatgtgccc acacacatca
     1561 ccatcgggcc gagcacctca tcccggatat aaaagtagag atgagtgaat cgtgacggcc
     1621 gcttgcggat cggccgatag ttcgggtctt cagtggcata cttcggattg agggattgga
     1681 agctgaagcc ctgctccata ctttggagga tgaagtacac gccatggcga ttgcggcgct
     1741 gcatcctttc cagatgaggc cggacgtaat cttcctttcg gactcccttc tgcgcccatt
     1801 ccatcgggat actgtggttc cgggcgaaag cttccaccca atgcttgtat tcgccggttc
     1861 tcttggatag cacctccttc gtgattggat acaggccctg aacgtcgcgg aagaaatgca
     1921 cgatgtgttc ttcgcgcgtc agcaatggca ggtatccctg aacgacaatg cggtcaaaac
     1981 agtggtatac aaaagcgagc aggctgccaa acagcttgtg gaacgtttcc atcggtcggt
     2041 tcctcgtgat cttgccttga caacaaaatc atcgatcagc cgaccgacgg aatcaacccc
     2101 tacgcagccg caaccttcgc gttttcaaaa tttcaaatat ttttggctag atttagcaac
     2161 tcaaaccgct tgcttacgca cgcggttctg ttatgcgtta tagctgccgg aggagacgcg
     2221 gcctccccgt ccggtccagt tcgtatggaa gaattcgccg cgcggccggt caacacgctc
     2281 ataggtatgc gccccgaaga aatcgcgctg cgcttgcaag agatttgcgg gaaggcgttc
     2341 gg
//
LOCUS       DATMTI010001066         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_167708, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001066 DATMTI010000000
VERSION     DATMTI010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167708"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VN039_16865"
     CDS             <1..656
                     /locus_tag="VN039_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HWV47671.1"
                     /translation="IATHLSALIKRHGHELLGRQEVQALLDEVGKAHPKLVEELIPTL
                     LPLGTVVRVLGNLLKEGIPIRDLRSILEAISDQATNTKDAEVLTEYARQALARTITKQ
                     YQASDGSLQVITLDPRLDRSLAEQAAALPPGAMLNLDPTLSHKLLTGLKQAAERVAAR
                     GQQPIVLCSQVVRRHLRRHSDRTLHAVPVMGLNEVDSFVRLQSLDTVRIDLELAQPS"
     gene            665..2005
                     /gene="flhF"
                     /locus_tag="VN039_16870"
     CDS             665..2005
                     /gene="flhF"
                     /locus_tag="VN039_16870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HWV47672.1"
                     /translation="MKVKTFHALTMQDAMRDIKEELGPDAIILSAKEVREGGRPLKAF
                     NRPVLEVMAASDQDRQRFPQGREVQQVSPAKPSDAGSDSAPSAQTFQQTLHGMLKPDC
                     ETMTPTVGERTALLKPAAVHGKPNRLRHLHTVVNELGRLLEDLSRADGRTIGSLPSPM
                     PVWLRRSLIEQGMNPSTVDLLLNEAGKTEQVSGPRDDESMRRALHHEIVKRVRTSGPP
                     LKGEGYPSIGLLIGPSGAGKTASVAKLASHYRLEQRRSVALITFDTCRETAVEQLRRY
                     AKVVGVPFACALSARQVHEGLRRHTQVDLVLIDMPGIGPRDLVLAQELQRLLPKDAVT
                     THLVLPASTREQELCRITRCLSDLPQPRLLFTKLDETESFGTIFEVAHQTGVPLSYWS
                     IGRRVPGDIEVASSERLAAFLTAERSTHSQVFVNRSIQSSEAASTLLGAGTHHG"
     gene            1998..2894
                     /locus_tag="VN039_16875"
     CDS             1998..2894
                     /locus_tag="VN039_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HWV47673.1"
                     /translation="MDNPAGGRDMSFRTRKSVLASEGLSRDGGASTLVIAVSSGKGGV
                     GKTNVVANLAVALTRVGKRVLILDADLGLGNLDVLLGLVPRHTIEDVLVGTHTLDEIV
                     LKGPAGIHVLPASSGVPRLTALTEAQQLMIQEQLAQLTSEMDVLLIDTGAGIAPNVTF
                     FAAAADETMIVVSPEPTSLTDAYALIKVLARQYRERRFKVLVNQAKSPREAAEVFGKL
                     DVAVDHFLHVAVELVGAIPYDDYMHLAVMQQKALSELFPEAPAAQAFKRLAQQILQWP
                     RPGLPKSSVQLVWQRAATPAGN"
     gene            2919..>3365
                     /locus_tag="VN039_16880"
     CDS             2919..>3365
                     /locus_tag="VN039_16880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV47674.1"
                     /translation="MSKTASPHEQESFSAQGVRREQLIKEFAHVIRAMAHRLAFRLPA
                     YLDAEDLISVGTIGLMDAMDKYDPTREAKFKTYAEFRIRGAMLDEIRSMDWIPRSVHE
                     RIGLLQKTHTALMSRLGRPPQDEEVAEELKMSMEELDDFVMRARGAV"
BASE COUNT          713 a          948 c         1015 g          689 t
ORIGIN      
        1 cgatcgccac acatctttct gcattgatca agcgtcatgg ccatgagttg ttgggacggc
       61 aggaggttca agcactgttg gacgaagttg gaaaagcgca tccgaagctg gtggaggagc
      121 tcattcctac gctcttgccg ctcggaacgg tcgtcagggt tctcggaaac ctgctcaagg
      181 agggtattcc tattcgagac ctacgatcga tcctcgaggc catttctgat caggccacca
      241 acaccaagga tgcggaagtc ctcactgagt atgcgcggca ggcgttggcc aggaccatca
      301 ccaaacagta tcaagcctct gacggcagtc tgcaggtcat tactttggac ccccgactgg
      361 atcgatcgtt ggcggaacaa gcggcggcat tgcctcccgg ggccatgttg aatcttgatc
      421 ccaccctctc gcacaaactg ttaaccgggc tcaaacaagc cgccgaacgg gttgcagctc
      481 gggggcagca gccgatcgtc ctttgctcgc aggttgttcg ccgccatctc cgtcgacaca
      541 gcgatcgtac gctgcatgcc gtgcccgtga tgggactcaa cgaagtggat tccttcgtcc
      601 gtctgcagtc gttggatacg gtgcggatcg atctggagtt ggcgcagccg tcttagggta
      661 agccatgaag gtcaagacat ttcacgcgct caccatgcag gatgccatgc gcgacatcaa
      721 ggaagaactg gggccggacg ccatcatctt atcggcgaaa gaagtgcgcg agggagggcg
      781 tcccctgaag gcattcaatc gaccggttct ggaagtgatg gccgcctccg atcaagacag
      841 acaacggttc cctcagggta gggaggtgca acaagtttct cctgcgaaac cgtccgatgc
      901 cgggtcggat tccgcgccgt ccgcgcagac cttccaacag acactccacg ggatgttgaa
      961 gcctgattgc gagacgatga caccgaccgt cggtgagcgg actgccttgt tgaagccggc
     1021 cgcagtgcat gggaaaccga atcgccttcg gcatctgcac accgtcgtga atgaattggg
     1081 ccgcttattg gaagacctgt cgcgggctga cggccgaacg atcggaagtc tgccgtcgcc
     1141 catgccggtg tggctgcgcc gctcgctcat cgagcaggga atgaatccct ccaccgtgga
     1201 cttgctcttg aacgaggcgg gcaaaaccga acaggtctcc ggtccacgcg acgatgagtc
     1261 gatgcggcgc gcgctgcatc atgaaatcgt caagcgcgtg cgaacgagcg ggccgccgct
     1321 caaaggagag ggctatcctt ctatcggtct cctgatcggg cccagtggag ccgggaagac
     1381 cgcctctgta gccaagttgg cttcacacta tcgtcttgaa cagcggagat ccgtcgccct
     1441 tattaccttt gatacctgtc gggaaacagc ggttgaacag ctgcgtcggt atgccaaggt
     1501 tgttggagtg ccgttcgctt gcgccctgtc ggctcgacag gtccatgaag ggctccgccg
     1561 ccatacgcag gtagacctcg tgctgatcga catgcctggg atcgggccgc gggacctagt
     1621 attggcccaa gagctgcaaa ggcttctacc aaaggatgcc gtcacgacac atctagtcct
     1681 tccggcttcc actagggagc aggagctctg tcgtatcacg agatgcctca gtgatctgcc
     1741 gcaaccgcgg ctgctcttca ccaagctcga cgagacggaa tcgttcggca ccatctttga
     1801 agtggcccac caaaccggcg tgcctctttc ctattggagt atcggacggc gagtccccgg
     1861 agatatcgag gtagcctcgt cggagcgttt ggcggcgttt ctcactgcgg aacgctccac
     1921 ccattctcag gtgtttgtca atcggtcgat tcaatcatcg gaagcagctt ctacattact
     1981 gggagcagga actcaccatg gataacccgg ctggaggtcg tgatatgtcg tttagaacaa
     2041 gaaagtctgt ccttgcgtcg gagggtctga gtcgggatgg tggcgcgtcc acgcttgtca
     2101 tagccgtctc cagcggcaag ggaggagtcg ggaagaccaa tgtggtcgca aatcttgccg
     2161 ttgccctcac gagggttgga aagcgggtgc tgattttgga cgcagacctc ggcttaggca
     2221 atctcgatgt cctgctggga ctggttccac ggcatacgat cgaagacgta ttggtgggca
     2281 cacatactct cgacgagatc gtgctcaaag gtccggcggg cattcatgtg ctcccggcca
     2341 gttccggcgt cccacgcctt acggcactga cggaagctca acagctgatg atccaggaac
     2401 agctggcgca gctcacctcg gagatggatg tgttgttgat cgataccggc gcaggcatcg
     2461 cgccgaacgt gaccttcttt gccgccgcgg cggatgagac gatgatcgtc gtctcgccgg
     2521 aaccgacatc gctcacggac gcctacgcac tgatcaaagt cttggctcgc cagtatcggg
     2581 agcgtcgttt caaagtgctg gtgaatcagg ccaagagtcc ccgcgaagct gccgaggtat
     2641 tcggtaagtt ggacgtggcg gtggatcatt ttcttcatgt ggccgtcgaa ttggtcgggg
     2701 cgattcccta tgacgactat atgcatttgg cggtcatgca acagaaggcg ctctctgaat
     2761 tgttcccgga ggcaccggcg gcccaagcat tcaagcggtt agcgcagcaa atactccagt
     2821 ggccgaggcc tggtctccca aaaagttccg tacagctcgt ctggcaacga gcggcaacgc
     2881 cggcgggcaa ctgaaacacg atcgtacatg ggaagatcat gagcaagaca gcctcaccgc
     2941 acgagcaaga atctttttcg gcccagggag tcaggcgcga acaactcatc aaggagtttg
     3001 cccatgtcat tcgcgccatg gcccaccggc tggccttccg cctcccggcg tacttggacg
     3061 cagaggacct gatttcggtc ggcaccatcg gactcatgga tgccatggac aagtatgacc
     3121 cgacacgaga agcgaagttc aaaacgtatg cggagttcag aatccgtgga gccatgctcg
     3181 atgagattcg ttcgatggat tggatcccac gatcggttca tgaacgaatc ggcctgcttc
     3241 aaaagactca cacggcgctt atgagccgac tggggcgacc gccgcaggat gaagaggtgg
     3301 cggaagaact gaagatgtcc atggaagagc tggatgattt cgttatgcgg gccagagggg
     3361 cggtg
//
LOCUS       DATMTI010001067         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_718409, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001067 DATMTI010000000
VERSION     DATMTI010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718409"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VN039_16885"
     CDS             complement(<1..1144)
                     /locus_tag="VN039_16885"
                     /inference="COORDINATES: protein motif:HMM:NF015321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HWV47675.1"
                     /translation="MAETPPETKLSSSEPCGWVNPFDQQECTEVGDHFCIPRADHVCQ
                     FFERLLMHTKGTQYRKRFILRGWQSEEILRPLFGRVRWSDEHQTYVRRYRIAYIEMAR
                     KNGKSQLLAGIMLYLLFADGEHSAEIISVAKDRDQAGKVFDVASQMVLLQPILSKHAK
                     VIPSTKRITRPKTNGLYVVKAADGGRLLGGNPSGVAADEILAWPTESGREVWNALRSG
                     MGSMDRLQPLMVAATTAPAADESFGADLHKRMVRVLENPDTEPHTFAWIKNLPLDADI
                     YDERNWYLPNPGLADFLSLEEMRSMALEAVSDPVAELSFRRFQLNQTLGSEVHWMPMH
                     LWDECEGTIYGTCQATLDAFAGRDCWMGIDLAARQDLTSICYLFPDP"
     gene            complement(1104..1559)
                     /locus_tag="VN039_16890"
     CDS             complement(1104..1559)
                     /locus_tag="VN039_16890"
                     /inference="COORDINATES: protein motif:HMM:NF016975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HWV47676.1"
                     /translation="MGKRGPTGRPNHLKALEGVEESRINRDEPIPSVGAVLCPVELND
                     AARAVWNRLAPDLIQKKVLTCWDADEFANGCRIQALLNEALDTAESCDLVIPGSNDNL
                     VMHPAIRAVTALEATLRSVWSRFGLTPGDRAALKVDDGGDSSGDEALIV"
     gene            complement(1552..1704)
                     /locus_tag="VN039_16895"
     CDS             complement(1552..1704)
                     /locus_tag="VN039_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47677.1"
                     /translation="MPAKAQSDLSEAREHVDQAFDLVARGGDRVQQAIAHLAIGLKLL
                     MKELDG"
BASE COUNT          403 a          566 c          509 g          365 t
ORIGIN      
        1 tagggtcggg aaatagataa cagatcgacg tgaggtcttg ccgtgcggcg aggtcgatgc
       61 ccatccagca gtctcggccg gcgaaagcat ccaacgtagc ctggcaggtg ccgtagatgg
      121 ttccctcgca ctcatcccaa aggtgcatgg gcatccagtg gacttctgag ccgagggttt
      181 ggttgagctg gaatcggcgg aacgaaagtt cggccacagg gtcgctgaca gcttcgaggg
      241 ccatggaccg catctcctca agggagagga agtctgccag tccaggattc ggtagatacc
      301 agttgcgttc gtcatagatg tcggcgtcga ggggaaggtt tttgatccac gcgaacgtgt
      361 gaggttcggt atcggggttc tccaagaccc gaaccatgcg tttatgcagg tcggcgccga
      421 acgattcatc cgccgccggg gcggtggtcg cggcgaccat caagggttgg aggcggtcca
      481 tcgaccccat accggatcgg agggcgttcc atacttcccg cccggattcg gtaggccacg
      541 ccaggatttc atccgccgcc accccagagg ggttaccgcc caacaaccgt ccgccgtcgg
      601 cggctttgac tacatacagg ccgttggttt tcgggcgcgt gatccgctta gtagaaggga
      661 tcactttggc gtgcttcgat aggatcggct gcaacagcac catctgcgat gcaacatcga
      721 aaaccttgcc ggcctggtcc cgatcctttg ctacggagat gatctccgca gaatgctcac
      781 cgtcagcgaa cagcagatac agcatgatcc cggcgagaag ctgcgatttg ccgttctttc
      841 gggccatctc gatataagcg atccgatacc tacggacgta ggtttgatgc tcgtccgacc
      901 agcgcacgcg cccgaacagg gggcgcagga tctcctcgga ctgccaaccc cgcaaaatga
      961 accgcttgcg gtactgggtg cctttggtgt gcatgaggag gcgttcgaag aactggcaca
     1021 catgatctgc ccgcgggatg cagaaatgat cccctacctc ggtgcattcc tgctggtcaa
     1081 aggggttgac ccagccgcat ggctcagacg atgagagctt cgtctccgga ggagtctccg
     1141 ccatcgtcca ccttcaacgc agctctatca cctggggtta aaccgaaccg ggaccacacc
     1201 gaccgcagcg tcgcctcaag cgccgtcacg gcgcgtatcg cgggatgcat cacgaggttg
     1261 tcattggagc caggaattac caggtcgcac gactccgccg tgtccaaagc ctcgttcaac
     1321 agcgcctgaa tacggcatcc gttggcgaat tcgtcggcat cccagcaggt caacaccttt
     1381 ttttggatca aatccggggc cagccgattc cacacagccc gagctgcgtc attcaactcc
     1441 accggacaca acacagcccc aaccgaaggg attggctcat cccggttaat ccgggactcc
     1501 tcaaccccct ccaaagcctt caaatggttc ggccggcccg tgggtccgcg cttacccatc
     1561 aagttccttc attaggagct tcaacccgat agccaaatgc gcgatggcct gctgaacccg
     1621 atccccaccc cgcgcgacca aatcaaacgc ctggtcaaca tgctcccgag cctctgacaa
     1681 atccgactgc gctttagctg gcatggctgc tcctgataat cgaacacact tacgaacaac
     1741 atcgaacgat tgttcgaaaa aaccgttatt agacccaacc cgctattgga ctacaactag
     1801 cgttgtcaaa aaacttcaaa acctgtggcc attcacggca ggc
//
LOCUS       DATMTI010001068         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_39742, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001068 DATMTI010000000
VERSION     DATMTI010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39742"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VN039_16900"
     CDS             <1..270
                     /locus_tag="VN039_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47678.1"
                     /translation="TRMIIGIQQLGDGRVDPISVEYVERTITDLIIQVPDPTLYKKLD
                     NIGVNNTVEWLLYEVQGKPVSWSNGLPWQRYSSLFGGTLHVRRVE"
     gene            267..569
                     /locus_tag="VN039_16905"
     CDS             267..569
                     /locus_tag="VN039_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47679.1"
                     /translation="MTFELTEEQHRAIRHMPLVVEHCTQKAEEMLGLVNDMCKTELKS
                     KKWQTRPKTGHFGVLVTDDPDHSRPRAYVHPVDGHGIRIEMTHHVLLKAAAAMGGR"
     gene            566..1006
                     /locus_tag="VN039_16910"
     CDS             566..1006
                     /locus_tag="VN039_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47680.1"
                     /translation="MTAPFVDPVILPPDIETLASNYLTTALSPTPIATRLPSPTKDAD
                     TVNGFCRVEYGGGSKPNRFHWDVQIILHGYSPNEIEASLIARRSVALLSAARGQTVSD
                     GTDGWFVVGVMGVVAPHRLTDPDVILPRYRASVTWRVAGQQWNP"
     gene            1026..1625
                     /locus_tag="VN039_16915"
     CDS             1026..1625
                     /locus_tag="VN039_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47681.1"
                     /translation="MTNAPSNISVGQIAALSPTAGTGGLWRAPLGTALPTDTTTALTN
                     TYTGLGYIDEDGVTISIDRPSTPQYSWGGLKLASLQQHYNPSLSFKLVQVLDADVLKA
                     VHSDTNVTVTAATQSAGTITTVEWNPLLNVNSVWVVAGFYQSATFRLAIPDARITSTR
                     DMRLTHKALAVWDCTLELFPDSAGNFAYQIWDDGVVAVA"
     gene            1622..>1876
                     /locus_tag="VN039_16920"
     CDS             1622..>1876
                     /locus_tag="VN039_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47682.1"
                     /translation="MTAVAEPDTEKPYVGPVFTYQPENGGDPIVFPSADVLWEDVDGV
                     KPAVFLWRLRKLREPYQTFAFLERAKVPDEMAERVLNLDPD"
BASE COUNT          395 a          566 c          550 g          365 t
ORIGIN      
        1 acacgaatga tcatcggcat ccaacagttg ggggacgggc gggtggatcc catctccgtc
       61 gagtacgtcg aacgaactat cacggacctg atcatccaag tccccgaccc caccttgtac
      121 aaaaagttgg acaacatcgg cgtgaacaac accgtcgaat ggctgctgta tgaggttcaa
      181 gggaaacccg tctcgtggtc gaacgggctt ccctggcaac gctactcgtc tttgtttggt
      241 gggaccttgc atgtgcggag agtcgaatga cgtttgaact cactgaagaa caacaccgag
      301 ctattcggca tatgcctttg gtggtggagc actgcaccca gaaggctgag gaaatgctcg
      361 gcctcgtcaa cgacatgtgc aaaacagaac tgaagtcgaa gaagtggcaa acccggccta
      421 agacagggca tttcggtgtc ctggtcaccg acgaccccga ccatagtcga cccagggctt
      481 atgtccatcc tgttgatggg cacgggattc ggatcgagat gacgcatcat gttctgttga
      541 aggctgcggc ggcgatgggc ggaaggtgac tgcaccattc gttgacccgg taatccttcc
      601 cccggacatc gaaacgctcg ccagcaacta cctcaccacc gcactatccc caacccccat
      661 cgcaaccagg ctcccgtcac ccaccaagga cgcggacacc gtgaatggtt tctgccgagt
      721 cgagtatggg ggagggtcga aacccaaccg attccactgg gacgtgcaga tcatccttca
      781 cggctactcc ccgaacgaaa tcgaagcctc cctgatcgcc cgcagatccg tcgccctcct
      841 gtctgcggcg cgggggcaaa ccgtgtccga cggcactgac gggtggttcg tcgtcggggt
      901 gatgggtgtc gttgccccgc accgtttaac agaccctgat gtcattttgc cgcgctaccg
      961 ggcctcggtg acgtggcgtg tcgcaggaca gcagtggaac ccctaaagat gaaagtgaaa
     1021 cagttatgac gaacgcgccc agcaacatca gtgtggggca gatcgctgct ctgtccccga
     1081 cagccggcac aggtggtttg tggcgcgccc ctctagggac cgccctgccc accgacacca
     1141 ccaccgccct gaccaacacc tacaccgggt tggggtacat agatgaggat ggggtgacga
     1201 tctccatcga ccgcccctcc acgccccagt attcgtgggg cgggttaaaa ctcgcctccc
     1261 tgcaacagca ttacaaccct tcgttgtcgt tcaaactggt tcaggtgttg gacgcggatg
     1321 tgttgaaggc tgtgcattcg gacaccaacg tcaccgtcac cgctgcgacg cagtcggcgg
     1381 gaacgatcac gacggtggag tggaatccgc tgttgaatgt gaactcggtg tgggttgtgg
     1441 ccggtttcta ccagtcggct acgttccggc tcgcgatccc cgacgcgcgt atcacttcca
     1501 cgagggatat gcggttgacg cacaaagcgt tggcggtgtg ggactgcacc ctggagttgt
     1561 tccccgacag tgcagggaat ttcgcctacc agatttggga tgacggggtc gtggcagtcg
     1621 cgtgaccgcc gtagcagaac cagacaccga aaagccctac gtcggaccgg tattcaccta
     1681 ccagcccgaa aacggtggcg atccgatcgt cttcccttcc gccgacgtgc tgtgggaaga
     1741 cgtagacggg gtgaagccag ccgtgttcct gtggcggctg cggaaactcc gcgagcccta
     1801 tcagacgttc gcgtttctgg agcgtgcgaa agtccctgac gagatggccg aacgggtcct
     1861 caaccttgac cccgac
//
LOCUS       DATMTI010001069         4762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_167744, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001069 DATMTI010000000
VERSION     DATMTI010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167744"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VN039_16925"
     CDS             <1..1446
                     /locus_tag="VN039_16925"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWV47683.1"
                     /translation="WLSPIFENNPHAYHGYNINNYLNLDAHFGTKQDLIDLVEAAHNF
                     QKDGQPFPIKIILDVVINHSGDNWEYAGGPKQYSGNTRYDFGFWRYDDRPIPTELRNP
                     EHYHRQGSITGDGWDSYPQNQFGDIVSLKDYANDDDPIGSEVINILIKAHCYWIREAD
                     VDGFRVDAVKHMNPLACSRFCSGIREYAYSLGKRGFFLFGELATPADEVVDRYIGQNT
                     SREDGNNTVFFGLDSILDFRLAEGNSGNAGLRDVIKGFAGPETLFNRLEAQRERALNR
                     GEIGRYLVTFLDNHDSFWQPGGRFGADAPDEQIIAGIGYLLCSLGTACIYYGTEQGFQ
                     GKGNDEAIREAMFDVKVVGRNLLNPDCKIYREISSIAEVMRTCEPLRFGRMYFRQISG
                     DGIHFGFPFGTTYTLAISRILYGREVLVAYNVSDQPRADSVIIDASLHDQGDKMTFLY
                     GKGGNTSVEQAPDGAHFVRLDLGPRQFVILQ"
     gene            1680..3263
                     /locus_tag="VN039_16930"
     CDS             1680..3263
                     /locus_tag="VN039_16930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HWV47684.1"
                     /translation="MPTNHNRVMVRGSERSSQHGARVVGPVPNDERFEVTVRIRRKTA
                     LQSLADGFHADKLPMNRRYLTREQYASSHGADPADMAKVEAFAQAHNLVVVEKSPARR
                     SIFLSGTTADFARAFGTTIEHYEHDGGTYRGRVGPLTVPAELDGIVEGVFGIDERPVA
                     KPHFQRRKMTGDMQPRATGESFTPVELATLYNFPSDLDGSGQCIGIIELGGGYRTADI
                     KAYFQKLGRPSPKVTTVRIDGGKNQPSNAQSADGEVMLDIEVAAAVAPKALIAVYFAP
                     NTDKGFLDAVTMAIHDTTHKPSVISISWGSAEDNWTEQAMTSFDQAFQTAAALGVTVC
                     CAAGDNGSADGEKDGKAHVDFPASSPFALGCGGTKLTASGNTIANEVVWNESADSATG
                     GGVSDFFPVPDYQSTVGIPPSANPGSHKGRGVPDVAGDADPATGYVVRVDGEEFVIGG
                     TSAVAPLWAGLIALMNQKLSHPVGFLNPLIYQPLVGKGSFRDISSGDNGAYSANQGWD
                     ACTGWGTPNGAKLLQALGG"
     gene            complement(3439..>4762)
                     /locus_tag="VN039_16935"
     CDS             complement(3439..>4762)
                     /locus_tag="VN039_16935"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV47685.1"
                     /translation="DPMGMELSDMYVLLKPESEWRAQSKTEIEEQVRRRLEQVPGIAF
                     GLSQPIAMRVDELVSGVRSQVAVKLFGDDLETLRVKADQISRVLRKVRGISDLRVEQV
                     SGLYYLKIDIDRAKIARHGINVAAITEVIEAVGGGIAVGEVFEGQWRFPIMVRFPDDR
                     RTDMETIAALWVTAPDGSRIPLRELADIRIVEGPAQISREHASRRIVIEANVVGRDLV
                     GAVEEGQAGVARLVQLPPGYYLTWGGQFENQQKAMARLAVVVPLVIGLIFLLLYFTFG
                     TLRHATLILLAIPFAMVGGLVALLVGGLYLSVPASVGFIALFGVAVLNGVVKIAYITQ
                     LREQGMPLDEAVLTGMVLRLRPVLMTATVAALGLTPLLFASGPGSEVQRPLATVVIGG
                     LISSTALTLVVLPVLYRWVEQRIVQRYEADYQTLARRSPTEATPHPLG"
BASE COUNT         1065 a         1409 c         1331 g          957 t
ORIGIN      
        1 tggctctcgc cgatctttga gaacaatcca catgcctacc atgggtacaa cattaacaac
       61 tatctgaact tggacgccca tttcggcacc aaacaggatc tgatcgacct tgtggaggcc
      121 gctcacaatt ttcagaagga cgggcagccg tttccgatca aaatcattct cgacgtcgtc
      181 atcaatcact caggtgataa ttgggaatac gccggtggtc ccaaacaata ctccggcaac
      241 accagatatg atttcgggtt ttggcgatac gacgaccgcc cgattccgac agagcttcgc
      301 aatcctgagc actatcatcg ccaggggagc attaccggcg atggatggga ttcctatccc
      361 cagaaccagt tcggggatat cgtgtccctc aaggactatg cgaatgatga cgatccaatc
      421 ggttctgagg tgatcaatat tctcatcaaa gcgcactgct actggatccg tgaagccgat
      481 gtggatgggt ttcgagtcga tgcagtcaag catatgaatc ccctggcctg ctcgcggttc
      541 tgttccggca tccgcgagta tgcctactcg ctcggcaaac gagggttctt tctctttggc
      601 gaattagcca ccccggctga tgaagtggtt gatcgctata tcggtcagaa cacatcgcgt
      661 gaagacggca acaacacggt gttcttcggc ctcgactcca tcctggattt tcgtttggcg
      721 gaaggtaact ctggaaatgc cggcctacgg gacgtcataa aaggatttgc cgggccggag
      781 acgctcttca atcgcctgga ggcgcaacgg gaacgagcac tgaaccgtgg ggaaatcggt
      841 cgttacctgg tcacgttcct ggacaatcac gattcatttt ggcaaccagg gggacgattt
      901 ggcgcggatg cgccggatga acaaattatt gcgggtattg gctatttgct ctgttctctc
      961 ggaaccgcct gcatctatta cgggaccgaa caaggattcc agggtaaggg caacgatgag
     1021 gcgatccgcg aagccatgtt cgacgtcaag gtagtcggcc gcaatctcct gaatccggat
     1081 tgcaagatct atcgtgaaat cagctccatt gccgaggtca tgcgaacctg tgaacctctg
     1141 cgcttcggtc ggatgtattt tcgacagatc tccggtgatg gcatccattt cggttttccc
     1201 ttcggcacga cctatacgct agcgatctct cggattttgt atggccgcga agtcttggtg
     1261 gcctataacg tttctgatca accccgtgcc gattcggtca tcatcgatgc atccctgcat
     1321 gatcaggggg ataagatgac ttttctctat ggcaaaggcg gcaatacttc agtggaacaa
     1381 gcacccgatg gagcccattt tgtccgactc gatctaggac cacgtcaatt cgtcatcctg
     1441 cagtagtggc ataggaggcc ggtcgtttaa ggaatgaatg gtatttcgtg ttgcagcggg
     1501 atccggagtg acgttacgtc ccaaaggctt aacgtggccg agagagtcgg tcaggttatc
     1561 tcgccctgat gggcaggcac ggatagcgag tccgtatggg aggttaacgg tccaccttgt
     1621 tcacgagggg cttcgggtct cttattgaca gaggccctat tcatgggagg gagcacatca
     1681 tgcctacaaa tcacaatcgc gtcatggttc gcggcagcga gcgcagttct caacacggag
     1741 cgcgtgtcgt cggtcccgtt ccgaacgacg agcgctttga agtgaccgtc cgaatccggc
     1801 gaaagaccgc tctccaaagc ttggccgacg gatttcatgc cgataaactg cccatgaatc
     1861 ggcgctacct cacacgtgag cagtacgcct catcgcatgg cgctgacccg gcggatatgg
     1921 caaaggtcga ggcattcgct caagctcaca acctcgtcgt cgtcgaaaag agcccggcgc
     1981 gacgtagcat ttttctctcg gggaccactg ccgacttcgc cagagcgttc gggacaacca
     2041 ttgaacatta cgagcacgac ggaggcacct accgtggtcg ggtcggcccg ctgacggtgc
     2101 cggccgagtt ggacggaatc gtggaaggtg tgttcggaat cgacgaacga ccggtggcaa
     2161 aaccacactt tcaacggcgc aaaatgaccg gcgatatgca accccgcgcg acgggagaat
     2221 catttacccc ggttgaactg gcaacgctct ataatttccc gagcgactta gacggctccg
     2281 gccagtgtat cggcattatc gagctgggcg gcggctatcg caccgccgac atcaaggcat
     2341 actttcaaaa gctcggccgg ccctcgccaa aggtcacgac ggttcggatc gacggcggaa
     2401 agaaccaacc ctccaatgcc cagagcgccg acggagaggt catgttggac atcgaagtgg
     2461 cggccgccgt cgcaccgaag gcgctgatcg cagtctactt cgcgccgaac accgacaagg
     2521 gatttctcga cgccgtcacc atggcgatcc acgacacgac ccacaagcct tcggtgatct
     2581 cgatcagctg gggcagtgcc gaggataact ggaccgagca ggcaatgacg tcgtttgacc
     2641 aggcattcca gacggcggct gcgctgggcg tgacagtctg ctgcgcggcg ggtgataacg
     2701 gatcggcgga cggcgagaag gacggcaagg cgcacgtaga tttcccggca tccagcccat
     2761 ttgcgctggg ctgcggaggc accaagctca ccgcctccgg aaacacgatc gccaatgaag
     2821 tcgtctggaa tgaatcagcc gatagtgcga ctggtggagg tgtgagcgac ttctttcctg
     2881 tcccggacta tcagagcacg gtcggcatcc cgccatcggc aaaccccggc agccacaagg
     2941 gccgtggcgt ccctgatgtc gcgggcgatg cagaccctgc caccggctat gtggtccgtg
     3001 tcgacggaga ggagtttgtg atcggcggaa caagcgcggt cgctccctta tgggccggct
     3061 tgatcgcgct gatgaaccag aaactcagtc atccggtagg gtttctaaac ccgctcatct
     3121 atcagccgct cgtcggcaag ggctccttcc gagatatctc ttcgggcgac aatggagcct
     3181 actctgccaa ccagggctgg gatgcatgca cgggatgggg tacgcctaac ggagcaaagc
     3241 tcctccaggc gctcggaggg taatccgaag gacggagccg gaccaggcgc ttgacccagc
     3301 taagcgcggc gagatcacag aatttatcaa tccttctcgc cgcacgaggc cggtgcgctt
     3361 ccctcgaatc gaacgcgcct cttcccaagt tcgtcacgaa ccttcgtgaa gaatgcaagc
     3421 tagtcatgac acatagcctc aaccgagggg atggggtgtc gcttccgtcg gagaccgccg
     3481 ggcaagcgtt tggtaatcag cttcgtaccg ttgaacaatc cgctgttcca cccatcgata
     3541 caaaaccggc aacacgacca aggtcaacgc cgtagacgaa atcaatccac cgatgacgac
     3601 agttgccaat ggtctttgga cttcggaacc cgggccgctt gcaaagagta acggcgttaa
     3661 tccaagtgcc gccacggtcg cggtcatcag cactggacgc aaccgcagaa ccatgccggt
     3721 caacaccgcc tcatccaacg gcatcccctg ctcccgcagc tgggtgatat aggcaatctt
     3781 caccaccccg ttgagcaccg ccacaccgaa caaggcgata aacccgacgg acgccgggac
     3841 cgacaggtac aggccaccga ccagcagcgc cacgagacct cccaccatgg cgaagggaat
     3901 cgcgaggaga atgagcgtcg cgtgccgcag cgttccaaac gtgaaataga gcaacaaaaa
     3961 gattaagccg atgaccagcg gtacgaccac ggctagacgt gccatggcct tctgctgatt
     4021 ttcgaattgg ccgccccaag tcaaataata gcccggcgga agctgcacca gacgtgcgac
     4081 tcctgcttga ccctcttcta ccgcgccgac tagatcgcgc ccaaccacat tcgcttcgat
     4141 cacgattcgg cgactcgcat gctcccggct gatttgcgcc ggtccttcca caattcgaat
     4201 gtcggccagc tcgcgaagag gaattcgtga gccgtccggg gccgtcaccc acaaggccgc
     4261 aatcgtctcc atatcggtgc gccgatcgtc gggaaaccgc accatgatcg gaaagcgcca
     4321 ctgtccttcg aaaacctcgc cgacggcaat gccgccaccg acggcttcaa tcacttccgt
     4381 gatcgctgcg acgttgattc cgtgacgcgc gatcttggca cggtcgatgt cgatcttcag
     4441 ataatataag cccgacacct gctcgactcg taggtctgaa ataccgcgta ctttccgcag
     4501 cactcgtgag atctggtccg ccttcacccg caaggtctca aggtcatcgc cgaacagttt
     4561 gacggcgacc tgagatcgga cccccgagac cagttcatca acccgcatgg cgatcggttg
     4621 ggacagcccg aacgcgatgc cgggcacttg ctccaaccgt cggcggacct gctcctcgat
     4681 ctcagttttg gactgtgccc gccactcgga ctcaggcttg agcaatacat acatgtcgct
     4741 gagctccatc cccattggat cg
//
LOCUS       DATMTI010001070         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_82040, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001070 DATMTI010000000
VERSION     DATMTI010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82040"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VN039_16940"
     CDS             complement(<1..1661)
                     /locus_tag="VN039_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWV47686.1"
                     /translation="MSILANKDTRVVVQGGAAGVNAARRMAEFCYLIKRPLNVEAFVY
                     PPDAGKTNEIPYGSGLIAIPIYKTIAEATKHHPTINTSLVYIGADRAMKGGMEALDDP
                     HIKVVSMITEGVPEKDAKLLGAHARKLGKVFNGPSSIGIISAGACRLGVIGGAFDNLV
                     LSKLYREGSFGVITKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRVKLIGVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKSGAIVPPTFGALGPAIKDAYQELLKSGQVKEPVEPAVLP
                     RLPKSIEEAMKADEVMVAPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVIGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQAVAAGLIMIGPRF
                     GGAVTDAGRWFKYAVDNKMSVDEFLAYMKKNVGPVPGIGHRVKSLRNPDKRVKELVGY
                     VKSLHIKTPCLDFALAVEQVTAVKKDNLILNVDGTMAAVLVDLGFP"
     gene            complement(1744..>2944)
                     /locus_tag="VN039_16945"
     CDS             complement(1744..>2944)
                     /locus_tag="VN039_16945"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HWV47687.1"
                     /translation="MAKVLEGPGMGLMKKWGIHVPHYVVVTSAEELSKLGHVNEWMKQ
                     TKLVVKAHEALGSRFKLGLVKVGLDLNGAVAATKEMIGRQVGSITISQVIVSEMVPHK
                     DEYYCAVKSTREGAEILVANCGGIEVESNWERVKRLCLDVGQAPSPEQLEKLAKDAGF
                     TGSVAKKMADFAGKMFACFDNEDAQYLEVNPVVTRESDGELIALDAVTLLDGDAKFRH
                     PDWNFQFAAEFGRAYSKDEVAVMAVDGKIKGSVKFIEIPGGDTAMLPAGGGASVYYSD
                     AVVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIKNIIVGGAIANFTDVKKTFGG
                     IINGFRKAKGDGKLKGVKIWVRRGGPREKEGLDAMRALKDEGFDIHVFDRNTPLTDIV
                     DKALQKQ"
BASE COUNT          589 a          926 c          781 g          648 t
ORIGIN      
        1 gggaacccca gatcgaccag cacggccgcc atcgtgccgt ccacgttcag aatcaaatta
       61 tccttcttca ctgccgtcac ctgctccact gccaaggcga agtcgagaca gggcgttttg
      121 atatgcaagc tcttcacgta acccacgagt tccttcacac gcttgtcagg attgcgcaag
      181 ctcttcactc ggtggccgat accaggtaca ggtcccacat tctttttcat gtaggccagg
      241 aactcgtcca cactcatctt gttgtcgacc gcgtacttaa accagcgacc ggcgtccgtg
      301 actgctccgc cgaagcgagg tccgatcatg atcaagccgg ctgccaccgc ttgggacagc
      361 ccgatcccgg cacaggccgc aagaatcgtc gcgtaggcgc cgctgacgca ggggccgtgg
      421 tcggcggaga gcatcatgat gcgcttgatg atttccgctt cctgcttcga gatcagccgc
      481 ttgtcccaga gaagaccgat gacatgggga atttcatagc ccttgttgat gagctcggag
      541 gccggataac cgtcgtagca aggttcgtcc cctcgatcgt cgctgatcgt ggtacggatg
      601 agcggtgcga ccatcacttc gtcggccttc atcgcttctt cgatggactt gggcaaccgc
      661 ggcaggacag ccggttcgac cggctccttg acctgaccgg acttgagcaa ttcttgatag
      721 gcgtccttga tcgcgggacc gagcgcaccg aatgtcggcg ggacgatcgc gccggatttc
      781 ttgagcgcgt cggacttcga gcgggcggaa ccttcgccct tcatcccttc cttggcgccg
      841 gcatggccga acttcatccc cttgggcaaa ctctcctgac agaatccgga gacgacgccg
      901 atgagcttca cccggcgctt tttcgcgccg taccactcgg cggcccgctc ttcgagatca
      961 ccgcccattt caccaacgat gacgaccgcc ttcgtctgcg gatcattctc gaacatttcg
     1021 aggtagctca catagtcggt gccgggataa gcatcgccgc cgatgccgat ggccgtcgtg
     1081 atgccgtcgg cgaactgcga acaaatccaa atgatttcgt tggaaagacc gcctgacttg
     1141 gtgatgacgc cgaaggaacc ttcccgatag agcttggaga ggacaagatt gtcgaaggcc
     1201 ccgccgatca cgcccagccg gcaagcgccg gcggagataa tgccgattga agaggggccg
     1261 ttgaatacct tgccgagctt acgcgcatgg gcgccgagca gtttcgcatc tttttcgggc
     1321 accccttcag tgatcatcga gaccacctta atatgtggat cgtccagcgc ttccatcccg
     1381 cccttcatgg cacgatccgc gccgatatag acaaggctcg tgttgatggt cggatgatgc
     1441 ttcgtcgctt cagctattgt cttgtagatg gggatcgcga tcaaaccgct gccgtaggga
     1501 atctcgttgg tcttgccggc gtcaggcggg taaacgaacg cctcaacgtt gagggggcgc
     1561 ttgatcagat aacagaactc cgccatgcgc cgggcggcgt tgacacctgc cgcaccccct
     1621 tgaaccacca cgcgggtgtc tttattcgcc agaatactca tcgcaatctc ctctttcctt
     1681 caggactgag tgctgaggac ggagggacga ggaaaggccc actcactcag tcctcagtcc
     1741 tcgttactgt ttttgaagtg ctttgtcgac gatgtccgtc agcggggtgt tgcggtcgaa
     1801 gacgtggatg tcgaagccct cgtccttcag cgcgcgcatc gcgtcaagcc cctctttttc
     1861 acgaggccca ccgcggcgca cccaaatctt gacacccttc agcttgccgt cgccctttgc
     1921 cttgcggaat ccattgatga tgccgccgaa ggtcttcttc acgtcggtaa aattggcgat
     1981 cgccccgccg acgatgatat tcttgatacc gggcaacgag cagacctttt ccgtcagcac
     2041 ttcaaccgcc cagtcggggg gatcacccga atactcggca tagttcgcca acttgccgcc
     2101 ccgcgccacg acggcgtctg agtagtagac actcgcgccg ccgcctgccg gaagcatcgc
     2161 cgtatcgccg cctggaattt cgatgaattt gacggacccc ttaatcttgc cgtcaaccgc
     2221 catgactgcc acttcatctt tgctgtaggc acgcccaaat tccgcggcaa actggaaatt
     2281 ccaatccggg tgacggaatt tggcatcgcc gtccagcagc gtcacggcat cgagtgcaat
     2341 caactcgccg tcgctctcgc gtgtcacgac cggatttact tcgagatact gcgcatcttc
     2401 attgtcgaaa caggcaaaca tctttccggc gaagtcggcc atcttcttgg cgaccgagcc
     2461 ggtaaatcct gcatctttgg cgagcttttc aagttgctcc ggggaaggag cttgcccgac
     2521 atcgagacac aaccgcttca cgcgctccca attggactcc acttcgatac cgccgcaatt
     2581 agccacaagg atctcagcac cttcacgagt ggacttcacc gcgcaatagt attcgtcttt
     2641 gtgggggacc atctcagaga caatgacctg cgagatggta atgctgccga cctgacggcc
     2701 gatcatttcc ttggtcgccg ccacggcacc attcagatcg aggccgacct tcaccagacc
     2761 gagcttaaac cgggacccca gcgcttcatg cgctttaacg accagtttcg tctgtttcat
     2821 ccactcgttg acgtgaccaa gcttggagag ttcctccgcg gaggtgacaa ccacataatg
     2881 tggaacgtga attccccact tcttcatcag tcccatcccg ggaccttcca aaacctttgc
     2941 catg
//
LOCUS       DATMTI010001071         3491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_82098, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001071 DATMTI010000000
VERSION     DATMTI010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82098"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..776
                     /locus_tag="VN039_16950"
     CDS             117..776
                     /locus_tag="VN039_16950"
                     /inference="COORDINATES: protein motif:HMM:NF037888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47688.1"
                     /translation="MGNTKVPVTPSSREVTDTLASIAVEHYHDFKQFVGIYYESPEQI
                     AILNATAPGFFADLHRWLIDRIVLNVRKMLDPAQTMGKTNLTVPALHQILTAEPKYPV
                     VEAQALIEQLRSLAVHVESWRHHLVAHIDRETALAPTAGCHMFVPSEISEFYKSLEAY
                     FDLIYRMLRDTVHPIDAVSHSDTHELMRALRDATVMADLLERDVTAYDALQRQSRFGR
                     K"
     gene            994..1290
                     /locus_tag="VN039_16955"
     CDS             994..1290
                     /locus_tag="VN039_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWV47689.1"
                     /translation="MAKTLPLSEVKTRLPELVAAVQEREEEVVVTKNGRPAAVLINVD
                     EYARIKETLDVLSDPALMSQIAESRAFYRRKNKGLSFEDVFGEPLPPIKKRRTA"
     gene            1287..1580
                     /locus_tag="VN039_16960"
     CDS             1287..1580
                     /locus_tag="VN039_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWV47690.1"
                     /translation="MNLFRPDIPPHVAEMIRLLHPDLKRSIKFAIRAIAADPECEEPL
                     QRELNGLRKYRVRRFRIVYAVDQKARVIRLMAVGHRRHVYEELTERLKRKGRE"
     gene            2205..2576
                     /locus_tag="VN039_16965"
     CDS             2205..2576
                     /locus_tag="VN039_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47691.1"
                     /translation="MTQIIRSGAFLQQCWSVHPLCVTVKRMTDENVVVLSCSSCKSAH
                     YLTIATVTSQASSAQHRAGSDACQDEPSGEVFLKACVSTHQALLTLREMDVFQDLVRL
                     RCADCRRQYELTVSAFETRHK"
     gene            2736..3344
                     /locus_tag="VN039_16970"
     CDS             2736..3344
                     /locus_tag="VN039_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47692.1"
                     /translation="MLFTDEEATLLADAQFFRKKAAITTKIRAMLEATHGALQNELAG
                     LSLVVPSDFDPTLHQLVKGEHLEDFPYQYLDCPKHFSGANKFTFRTLVWWGHHVTCAL
                     LLEGSELGRYKRQLLGRFHQLAGRDLELSLAPSLWEWKRGEGYTLPITHDRKAQIAAV
                     VAERSFLKVVRCVPLEDPRVREGRLAGMACETFRSMHPLVTP"
BASE COUNT          735 a          959 c          990 g          807 t
ORIGIN      
        1 ggaaggatgt ggcgtatcgg tccgggtgaa tcgtgggcgt tttatgcgaa taggaactcg
       61 aagatccagc tcttatacgg atctgtccga atcgttaggc ttacggatat agaacagtgg
      121 gaaacacgaa ggtgcccgtc actcctagtt cacgcgaggt aaccgacacg cttgcgtcga
      181 ttgcagttga acactatcac gacttcaaac agtttgtggg aatctactac gaaagtcctg
      241 agcagatagc gattctgaat gccaccgctc cagggttctt cgccgacctt catcggtggc
      301 tcatagatcg gattgttctg aacgtcagga agatgcttga cccagcccaa acgatgggta
      361 agacgaatct gacggttcct gcgctgcacc agatcctcac ggcagaaccg aagtaccctg
      421 tggtggaggc gcaagcattg atcgagcagc tccggtctct cgccgtgcac gtggagtcat
      481 ggaggcatca cctcgttgcg cacattgacc gcgagacagc cctcgcccca accgcaggat
      541 gccacatgtt cgtgccaagc gagattagcg agttctacaa gtcgcttgag gcatacttcg
      601 acctgatcta ccgcatgcta cgggataccg tccatccgat tgacgcagtt tctcactcgg
      661 acacgcacga gttgatgcgg gcactccgtg atgccacggt tatggctgac ctccttgagc
      721 gcgatgtgac ggcgtacgat gcacttcaac gccagagtcg ctttggccgc aagtagacca
      781 atgtgtcaaa gctgggcttg cgtcttgcgg ctcagtcaca gcaccctctt ctcctctgtg
      841 tctcacagtt ccaatgtgaa tgagagtctc gcgttttacg tccgccctga tgcgcgtcgc
      901 gcagcgatca ccaccctctc aattgacgac atcccttctg ccaatgtatg atcatgacca
      961 gaattgtggt caatattgag agcgaggtgg gtgatggcaa aaacattgcc gttgtcggaa
     1021 gtgaaaaccc gtttacctga actggtggcg gcggtgcaag aacgcgagga agaagtggtt
     1081 gtgaccaaga acggccgtcc ggctgccgtt ttgatcaatg tcgatgaata tgctcgcatc
     1141 aaggaaacgt tagacgtcct cagcgatccc gcgcttatga gtcaaattgc cgagagccga
     1201 gcgttttata ggaggaagaa caaagggctg tcgtttgagg atgtgttcgg cgaacccctt
     1261 ccgcccatca agaagcggcg cactgcgtga acctgttccg tccggacatc cctccgcatg
     1321 tcgccgagat gatccggttg cttcatcctg acctgaaacg gtcgatcaag ttcgctattc
     1381 gtgccattgc cgctgaccca gaatgtgagg agccgcttca gcgggaactc aacggactgc
     1441 gcaagtatcg cgttcgccgc tttcgcattg tctatgctgt cgaccagaaa gcgcgagtca
     1501 ttcgcttgat ggccgtcggt catcgtcggc atgtgtatga ggaactgacc gagcgactca
     1561 aacgaaaggg acgagaatag cgagtgttgc ggctgtgatg catctgaagc gaaaaccggg
     1621 ctattgggtt tcacgcggca aggtcaagta gcgtctcctc ttcgcttgtt ttgcgtcggt
     1681 agccccgcta tacttcatac tcttatgagt attcgaaaag gcggcggtga gtgggagccg
     1741 atgctgctcg gggtcgaggc ccgcaattgt aaagtctgca aagagggcct ctatcgggac
     1801 aagacgctct tgcgcggtgg gtggtcccgt tgcactatct gcgacgagtt cgtccattac
     1861 agttgcctcg ccagtggaaa agtctccttt ctgaaagccc gaccacgggt gtgcaagact
     1921 tgccgttcgg tacaggaggg cgtcgtccag ccttctctca agaagggtga gccccctgtg
     1981 gcagccttgt catgaattcc ggttcgccgt cccgccttgc gtgcttacgc cagggacggc
     2041 tcggcgtctt ctccaaaacc attcagtgtg tgaaggtcct gttgctcttg ctggccggca
     2101 tcgggcaaaa gcggttctgc tctccttctg tgtgatcaga atgcccggtc agctgaacgt
     2161 tggtgagcga gcctcaagcc gcgtgacggt ttattgagac gagtatgacg caaatcatcc
     2221 gaagcggagc gtttcttcaa caatgttggt cggtccaccc gctgtgtgtg acagtcaagc
     2281 gaatgacaga tgagaatgta gtggtgctct cctgcagttc atgcaagtcg gcccattacc
     2341 taacgatcgc cacggtcacg tcgcaagcat cctcagcaca acatagggcg ggaagcgacg
     2401 cgtgtcagga cgagccgtct ggtgaggttt tcctcaaggc ctgtgtctcg acccatcagg
     2461 ccttgctgac actccgtgag atggatgtat ttcaggatct ggtgcggctg cgctgcgctg
     2521 actgccggcg gcagtacgaa ctgacggtct cggcgtttga aacgcgccac aaatagcttg
     2581 aggatgttgc aaaaactctc cagcgtcgtt ctcgcatcgc tcaaggcctc aacgtaccaa
     2641 ccgcgtacgc ctcggccttt cgctcgctgc ggccttgctg gacgagcttt ttcatcatcc
     2701 tcgtgaacca accgcgtgcc ataactgccc agtctatgct gttcaccgac gaagaggcca
     2761 cgctgttggc cgacgcacag ttctttcgga aaaaggctgc gattacgacc aaaatacgag
     2821 ccatgcttga ggcgacgcat ggcgcattgc agaatgaact ggccggtcta tcgctcgtcg
     2881 tcccttccga tttcgacccc accctccatc agctggtaaa aggcgagcat ctggaggatt
     2941 tcccgtatca ataccttgat tgtcccaaac acttctccgg cgcgaacaaa ttcacgtttc
     3001 gtacgctggt ctggtgggga catcatgtga cctgtgcgct tttgctggaa ggttcggagc
     3061 tggggcgata caagcgccag cttctcggcc ggtttcacca gctcgcggga agagacctag
     3121 agctttctct ggcaccgtcg ttatgggagt ggaaacgcgg ggagggctat acactgccga
     3181 tcactcatga ccgcaaggcc cagatcgctg ccgtggtggc tgaacgatcg ttcctgaagg
     3241 tcgttcgctg tgtgccctta gaagacccac gagtacggga ggggcggttg gccgggatgg
     3301 catgcgaaac ctttcgatca atgcaccccc tcgtcacccc ttaacttttt tatcctaatt
     3361 gtctctgaca ccaaacttgg gtagactggg ccgccattct ttttgaacct tgacagggag
     3421 gagcccgtga agcaatcgtc attgcgagtt ctgggctgtg ccatcatgat ggcgaccatg
     3481 ctggccggct g
//
LOCUS       DATMTI010001072         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_507691, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001072 DATMTI010000000
VERSION     DATMTI010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507691"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VN039_16975"
     CDS             <1..799
                     /locus_tag="VN039_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47693.1"
                     /translation="VQVSNLVAGSLGQVWKDTIRTLNPGTYRLWSMVNRDHYWLFLEN
                     VAPTFTITKGNISTAPTKLTIVINMVTRALVLATNVDIRGATIVPASAGRQAWYLVND
                     AAVGHVCDGDKLFTTTGVDSITCDGNVLGPQIYMESKKFDAGDGMRLKRWKLVSMNYL
                     AQGGNLILDVVLGLNEVGQVASTNFPQSVMTWTQLRSNITTWTNLKNQYSTWSHITQS
                     VFIPARIRMQKKSQYMSFRLYGSVANLAKAEIGPYAVDYKLQRVGRV"
     gene            799..1311
                     /locus_tag="VN039_16980"
     CDS             799..1311
                     /locus_tag="VN039_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47694.1"
                     /translation="MPVFSTDKIGDWDITVLTKFLEDWFRNHPLPPIQQFSVDTLKVN
                     TLLDVVNKVQFDALDVRVVGVTGAAAYQNGWHAYGAPYAKAAYVKTPDGWVRLFGVIA
                     GGTVGSSAFILPPGYRPDDSALPGPFAVFTSNANPIGRVDIQAGGAVTPISPSVNVGV
                     SLEGITFQAA"
     gene            1324..2079
                     /locus_tag="VN039_16985"
     CDS             1324..2079
                     /locus_tag="VN039_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47695.1"
                     /translation="MPTDLTGTPTSLGIGTYNVDADAPSGLGFNAAMDQIDALIAARI
                     GTPAGIATGEIPVWNGSTWVRSSTLRPAQSSLATGTPAITTFLRGDGAWQKGGLNLLW
                     DSVDAGVTLPAASITTPSLAQSFKSLLVTYQYQQNSGASQNLYVRPNSDATAVNYDGV
                     SMHATGSTLTVNHDTVSSAWVIGNGSFGTGQFLINNYAGGTNHMFQGTYSEELHLWTV
                     SGYWAGVAPITTLNFFPASGSFVSWARISVYGI"
BASE COUNT          464 a          603 c          590 g          481 t
ORIGIN      
        1 cgtgcaggtc tcgaaccttg tggccggttc gcttggtcag gtctggaaag acacgatccg
       61 gacgctgaac cccgggacat accgcctgtg gtcaatggtg aacagagatc actactggct
      121 attcctggag aatgtcgccc cgactttcac gatcacgaag ggcaatatca gcactgctcc
      181 gaccaagcta accattgtca tcaacatggt tacgcgggca ctcgtacttg caaccaatgt
      241 ggacattcgc ggtgcaacca tcgttcctgc ttctgcagga cgacaggcgt ggtatctagt
      301 taatgatgca gcggtcggac acgtctgtga cggcgacaag ctgttcacga cgacaggggt
      361 ggactcgatc acttgcgacg gaaacgtact tggcccccag atttacatgg agtccaagaa
      421 gttcgacgcg ggcgatggga tgcgtctgaa gcggtggaag ctcgtctcga tgaactacct
      481 cgctcagggt ggcaacctaa tccttgatgt cgtgctcggg ctgaacgaag tgggacaggt
      541 cgcctcgacc aacttcccgc agtccgtgat gacgtggacg cagcttcgct ccaacatcac
      601 gacgtggact aacctgaaaa accagtattc aacatggtct catataacgc agtctgtgtt
      661 cattccggct cgaatcagga tgcagaagaa gtctcagtac atgtcgttcc gtctatacgg
      721 ttcggtagct aatcttgcta aggctgagat tggcccgtac gccgtggact acaagctaca
      781 gcgggtgggt cgcgtctaat gccagtattt tctacagaca agatcgggga ttgggatatc
      841 accgtcctga ccaagtttct tgaagactgg ttccgcaacc acccgctgcc gcccatccaa
      901 cagttcagtg tcgatacgct gaaagtgaac acgctactcg acgttgtgaa caaagtgcag
      961 ttcgacgcct tggacgttcg cgtcgtcggc gttaccggag cggctgctta tcagaacggc
     1021 tggcacgcat atggcgctcc gtacgccaaa gccgcctacg tgaagacgcc tgatggctgg
     1081 gtacgcctct tcggtgtaat cgcgggcggt acggttggtt cgtcggcatt catcttgccc
     1141 ccagggtata gacctgatga tagtgctcta cccggcccgt tcgctgtgtt cacgtctaac
     1201 gccaacccaa taggtcgagt agatatccaa gccggtggcg ctgtgacacc aatttctcct
     1261 tccgtgaacg ttggcgtgtc attggaaggc atcactttcc aagctgccta aaggaggcaa
     1321 gacttgccaa ccgatcttac gggcacccct acctccctcg gcatcggcac gtataacgtc
     1381 gatgctgatg cgccgtctgg tcttggcttc aatgcagcca tggaccagat cgatgcgctg
     1441 attgccgctc gtatcgggac gcctgctgga atcgctaccg gcgagattcc ggtctggaac
     1501 ggctcgacct gggtgcgttc ttcaacgcta cgccctgctc agtcgagtct tgccacaggt
     1561 acgcctgcaa tcacgacgtt ccttcgcggt gatggtgcgt ggcagaaggg cggtctgaac
     1621 ctactctggg attcggtcga tgcgggggtc acactgccag cagcttcgat cacaacccca
     1681 tccctagccc agagctttaa gagcctgctc gttacatacc agtaccaaca gaactccggc
     1741 gcatcccaaa acctgtatgt gcgacctaat agtgatgcca ccgctgtcaa ctatgacggg
     1801 gtgtctatgc acgccacagg atcaacgctg actgtcaatc acgacacagt ttcttctgcg
     1861 tgggtgattg ggaacggttc gtttggcaca gggcagttcc taatcaacaa ctacgcgggc
     1921 ggcactaacc acatgtttca gggtacgtat tcagaggagc tacacctgtg gacggtaagc
     1981 ggttattggg ccggggtggc gccgatcact accctcaatt tcttccctgc ttcaggcagc
     2041 ttcgtcagtt gggcacgcat tagcgtctac ggcatctaaa ggaggctcgc atggacagac
     2101 cgatgatcaa tacctacgag ccggataccg aaggtgtc
//
LOCUS       DATMTI010001073         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_422526, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001073 DATMTI010000000
VERSION     DATMTI010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422526"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..378)
                     /locus_tag="VN039_16990"
     CDS             complement(103..378)
                     /locus_tag="VN039_16990"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HWV47696.1"
                     /translation="MLSEDKVSHLSHVILTAVKQHAGARVKSDDARLLKEIKRVLAAE
                     LAQEQEIDRKVKAKLASYSRGIIEGSNEWDVLYRKTFEEEMRKQTKV"
     gene            complement(481..762)
                     /locus_tag="VN039_16995"
     CDS             complement(481..762)
                     /locus_tag="VN039_16995"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HWV47697.1"
                     /translation="MRLSKERVRHMAESLTGRLQEEGHIELVGERKGFVEALDQAITH
                     ELSAEDRVNAEVRQLLKAYEQQIAQGQVDYQKMFQMVKQKLIRERGIIL"
     gene            complement(768..1151)
                     /locus_tag="VN039_17000"
     CDS             complement(768..1151)
                     /locus_tag="VN039_17000"
                     /inference="COORDINATES: protein motif:HMM:NF016050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HWV47698.1"
                     /translation="MTAPAKRIRITVGGVQLEAELRKTKTADEVYAALPVTAAISTWG
                     EEFYFPIPGVRDYRETATTQVKVGDIAFWGVGKVLAIFFGRTPMSLGADPVPADRVNI
                     IGRIVGDATQFRRVMKAPTIHLEAG"
     gene            1247..2038
                     /locus_tag="VN039_17005"
     CDS             1247..2038
                     /locus_tag="VN039_17005"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWV47699.1"
                     /translation="MLTSSFIFLPGVGPVTERRWWQAGLCNWQSFLDRPSIAGLSEHR
                     KIWYDDELREAQSLTEKGHMQMFGSRFRRREHWRLYKTCRSRAGYLDIETTGAPPESG
                     EVTVVGLHREGNTTTLVRGENLTTERLQAELDHCNLLVTFFGSVFDVPYLCAKFPGLR
                     FPTLHFDLCFAAHRLALRGGLKHIEQEMGIDRNTTISGLDGSDAVRLWFQWRRGDTIA
                     RDTLLSYNRADTENLVSLADRFYEDLVSRFGPTSLSNTLQPAYSR"
     gene            2035..2790
                     /locus_tag="VN039_17010"
     CDS             2035..2790
                     /locus_tag="VN039_17010"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWV47700.1"
                     /translation="MSTWIGIGRSEVGLVRTLNQDAFAVIDSVSVWAVADGMGGHVGG
                     EVAAQTAIATVQAEAAASSHLLGNGQQAPTDVLTDLINRAHDAILNRARSKSKLKGMG
                     TTIVLLAIVSGPAPIAYLVHVGDSRAYRFRSGTLTLLTKDHTLIEKYLERGILTAESA
                     KTHPERHVLTRALGVGATVKPTITAFPLLPEDLVLLCSDGLTKMLEDEEIRTVFAAGE
                     LDPTQVCNRLVTAALDRGGEDNVTVVVVAPRSS"
     gene            2881..3759
                     /locus_tag="VN039_17015"
     CDS             2881..3759
                     /locus_tag="VN039_17015"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV47701.1"
                     /translation="MPRRGSFVCGSLFLLIGLTTVAGAGEPSDPEAAIRQMVRANAEK
                     DLPTLSRLMAHDADIVSYSVGGRKYVGWLEFEREMREEFINAQKIEIPITELKVWTKG
                     DLAWFTMELDYTRYVGEGSSVKRTVLPLRETGVLERRAGRWQLLSWHESFRSAQLGGP
                     LASPLMTGGSQKLVNNAAAAAMPDVSGEWDILEVEDEKRYKATLDKNGNGPYTQQGGR
                     FTTTKIENRLWQGTWHQPGNDREGGFEVLLSEDGTQAKGIWWYTRVDTRKNIPPKEHG
                     GTYHWKKVTPSPANAQ"
BASE COUNT          809 a         1126 c          994 g          857 t
ORIGIN      
        1 gtacgcttct gcgaacatgc cgtcatgccg acaagcacca gcactatacc tacgattacc
       61 gctcggtgta gggtcttttt catggcaacg cgacctcagc cttcagacct tcgtctgttt
      121 gcgcatttct tcctcgaacg tctttcgata caacacgtcc cactcattac tcccttcaat
      181 aatgccccgg gaataggatg cgagtttggc ttttactttt cgatcaatct cctgttcctg
      241 ggccaactcg gcggccaaca cgcgcttgat ctctttcaac aaccgtgcat catcgctctt
      301 aaccctcgct ccggcatgtt gcttgacggc cgtgaggatc acatgggaca gatgactgac
      361 tttgtcctcg ctcaacatct tgagtccaag tcaatggtca ctcgtcaccg gccaacggca
      421 gctgaaactt tacgataacc gattcgtttc ttgcctcatg acccccatga ccattgactt
      481 tcacagaata ataccccgtt cacgaatcaa cttttgtttc accatctgga acatcttctg
      541 atagtcaact tggccttgtg caatctgctg ctcataggcc ttcagcagtt gccgcacttc
      601 cgcattcaca cgatcctcag cggataactc atgcgtgatg gcctgatcca atgcctcaac
      661 gaatcccttt cgctctccga ctagttcgat atgtccttcc tcttggagac gaccggtgag
      721 gctctcggcc atatgccgca cacgctcttt cgacagtcgc atcgcactca ccccgcttcg
      781 agatgaatcg tcggggcttt catcacccgt cgaaattggg tcgcatctcc aacgatcctc
      841 ccgatgatgt tcacccgatc tgccgggacc ggatcggccc ccaaactcat gggggttctt
      901 ccaaaaaaga tcgccagaac cttccctacc ccccagaagg caatgtctcc gaccttcacc
      961 tgagtcgtcg cggtttcccg atagtcgcgg actccgggaa tcgggaagta gaactcctct
     1021 ccccacgtgc tgatcgcggc cgtgaccgga agtgccgcgt agacttcgtc agccgtcttc
     1081 gtctttctga gttcggcttc cagctgaact ccacccacag taatacgaat tcgcttcgcc
     1141 ggcgcggtca tgcgatcgat ggcctactcg tttcttcact gcattcttcg cgatgctgcg
     1201 aactctagct tgtctcctat gcgaaatcaa ggctacaatc cctgaaatgc tgacctccag
     1261 cttcatcttt ctaccgggcg ttggaccagt cacggaacgc cgctggtggc aggcgggctt
     1321 gtgcaattgg caaagtttcc tggaccgacc gtccatcgcc ggcctctccg agcaccgcaa
     1381 gatctggtac gatgatgagc ttcgagaggc gcaatcgctg acggagaagg gacacatgca
     1441 gatgtttggc tctcgctttc gacgccgaga acattggcga ctgtacaaaa cctgccgatc
     1501 tcgggcaggt tatctcgaca tcgaaacgac cggtgcaccg ccagaatcgg gagaggtgac
     1561 tgtcgtggga cttcatcgcg aggggaacac gacgaccctg gttcgtggtg aaaacctgac
     1621 gacggagcgg ttgcaggcgg aactcgatca ctgtaatcta cttgtgacgt ttttcggatc
     1681 ggtctttgat gtcccctacc tctgtgccaa gtttcccggc ctccggtttc caacattgca
     1741 tttcgacctc tgttttgccg cccatcggct tgcgttacgt ggagggctga agcacatcga
     1801 acaagaaatg gggattgacc gcaacacaac catcagcggc ctcgacggat cggacgcggt
     1861 tcgcctgtgg ttccagtggc gccgaggtga cacgatcgcc cgcgataccc tcttgagtta
     1921 caaccgggcg gataccgaaa acctcgtgtc ccttgccgac cgcttctatg aggacttggt
     1981 ctcccggttc gggcctacct cattgtcgaa cactcttcaa ccggcctatt cccgatgagc
     2041 acctggatcg gcatcggtcg gagcgaggtc ggactcgtac gtaccctcaa ccaggatgcg
     2101 ttcgctgtca tcgactcggt gagtgtctgg gccgtcgcgg atggaatggg tggtcatgtc
     2161 ggtggcgaag tcgccgctca gacagccatc gccacggtac aggcggaggc tgcagcatcg
     2221 agccacctgc ttggcaacgg ccagcaggct cccacggatg tgttgacgga cctgatcaac
     2281 cgagcgcatg acgccatcct caatcgggct cgatcaaagt ctaagctgaa agggatgggc
     2341 acgaccatcg tgctgctggc gatcgtctcc ggtccggccc cgatcgctta cctcgtccat
     2401 gtcggcgaca gtcgtgccta tcggtttcga tcaggcacat tgaccctcct gaccaaagac
     2461 catacgttga tcgaaaaata cctggaacgc ggcattctca cggcagaatc agcaaaaact
     2521 catccggaac ggcatgtgtt gacacgtgcc ttgggggtag gcgccacggt gaaacctacc
     2581 attacggcct ttcccctgct gccggaggat ctggtcctgc tctgctcgga tgggctgacc
     2641 aagatgttgg aggacgagga gatccgaacc gtgttcgctg ccggcgagct cgacccgact
     2701 caagtgtgca accgcctcgt taccgcggca ctcgatcgtg gcggcgaaga caatgtcact
     2761 gtggtagtgg tcgctcctcg ttcgtcctga ttttacttcg ttgttgacat ggcccgtcag
     2821 gccgtgtagt tttcctgctc atttgaagct ttgaaccttg ccccgtccat ggagaccctt
     2881 atgccccgtc ggggatcgtt cgtctgcgga agtctgttcc ttctcatcgg ccttacgacg
     2941 gttgcgggcg ccggtgaacc gtccgacccg gaagcggcca ttcgtcaaat ggtccgggcc
     3001 aatgcggaaa aagatcttcc gacgctctct cgtctcatgg ctcacgatgc cgatatcgtg
     3061 agttactcgg tcggtggacg aaaatacgtc ggttggctgg agttcgaacg cgaaatgcgt
     3121 gaggaattca tcaacgccca gaagattgag atccccatta cagagctcaa agtctggacg
     3181 aaaggagacc tggcctggtt cacgatggag ctcgactata cccgctacgt cggtgaagga
     3241 tcctcagtaa agcgaacagt acttccttta agagaaaccg gtgtcctcga acgtcgtgcc
     3301 ggccgttggc aactattgtc gtggcacgag tcgtttcgat ccgcgcaact aggaggtcct
     3361 cttgcctcac cattgatgac gggcggatcg caaaagctcg tcaacaatgc tgctgcagcg
     3421 gcaatgcctg acgtgagcgg agaatgggat attctcgaag tcgaggacga aaaacgatac
     3481 aaggcaacgt tagacaagaa tgggaacggc ccctacacgc aacagggcgg acggtttact
     3541 acaacaaaaa ttgagaaccg actgtggcaa ggcacctggc accagccagg caacgatcgc
     3601 gaaggcggat ttgaggttct gctctcggaa gacggcacgc aagcaaaggg aatctggtgg
     3661 tacacacgag tcgatacgag aaaaaatatt ccgccgaagg aacacggcgg tacctaccac
     3721 tggaagaaag tgacaccctc accagccaac gcccaatgag tcagtttgtt tcttctcagc
     3781 catacc
//
LOCUS       DATMTI010001074         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_676928, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001074 DATMTI010000000
VERSION     DATMTI010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676928"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VN039_17020"
     CDS             <1..257
                     /locus_tag="VN039_17020"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWV47702.1"
                     /translation="FDGIDSEIARKTCPRNLWKVARRKLDQINRVKDLIDLAAPPGNR
                     LERLRADRIGQHSIRINDQYRVCFRWEAGDVYEVEITDYH"
     gene            265..615
                     /locus_tag="VN039_17025"
     CDS             265..615
                     /locus_tag="VN039_17025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWV47703.1"
                     /translation="MTTGLQGPSRALVGRRLPRRRPPTRPGDMLLEEFVMPLGITQSE
                     LAIRLGISFPRLNEIIRGKRSITSDTALRLAQVLGMSADFWLGLQMDWDLWHAIHGSK
                     ASEIHRLEPLRTSA"
     gene            complement(659..>1501)
                     /locus_tag="VN039_17030"
     CDS             complement(659..>1501)
                     /locus_tag="VN039_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWV47704.1"
                     /translation="TFHCQTETIPTYHLPETPALVLSKAAAYAEWRHQRPGMVPDFDD
                     LDLSAVRNICANACARCGAGWLSTEETRNVLSAIKVPVQAGGVAKTADEAAALATQVG
                     YPVAVKLASRQIVHKTEVGGVQLNLRDVQAVRKAFDSMRTRLAETNQLDAMEGVLVQP
                     MATGGVEVMIGVTDDSLFGPLIAFGLGGIHVEILGDVQFRITPLTDRDAAEMIRGIKA
                     YRLLTGYRGHPPADLEALEETLLRVSRLVEEIPEISELDLNPIFALPPGQGCRIVDAR
                     IRVG"
BASE COUNT          314 a          431 c          436 g          320 t
ORIGIN      
        1 tatttgacgg catcgattcc gagatagcta gaaaaacctg tcctcgaaat ctgtggaagg
       61 tggccagaag gaagctcgat cagattaatc gcgtgaaaga tctcatcgat ctcgctgcac
      121 caccaggaaa tcgccttgaa cggttgcggg cggaccggat cggacagcat agcattagga
      181 tcaatgatca atatcgagtc tgttttcgat gggaggctgg agatgtctac gaggttgaga
      241 tcacggatta tcactaagag gccagtgacc accggcttgc agggcccttc gcgagccctt
      301 gtcggacgtc ggctgcctcg tcggcgacca cccacgcgtc cgggtgatat gttgctggaa
      361 gagttcgtca tgccgctggg cataacccaa tcggagttgg cgatccggct gggtatctcg
      421 ttcccccggc tcaatgaaat catccggggc aaacgctcga tcacgtccga tactgcgctt
      481 cggctggcac aggttctggg gatgtctgcc gacttctggc tggggctaca gatggactgg
      541 gatctctggc acgccataca cggctcgaag gcaagcgaaa tccaccgcct tgagcccctt
      601 cgtacttcag cctgagcagg ttctacatgc attgactgag atgaggatct ggctctgcct
      661 agccaacccg tatcctcgca tccacgattc ggcagccctg gccaggggga agggcaaaga
      721 tcgggttcaa gtcgagttcg ctgatctccg gaatttcttc aacgagacgg gatacccgca
      781 gcagagtttc ttcaagagct tcaagatcgg ccggtggatg tccccgatag ccggtgagca
      841 agcgataggc tttgattccc cggatcatct ccgcggcgtc gcgatcagtg agcggcgtga
      901 tgcggaactg gacatcgcca agaatctcga cgtgaattcc tccaaggccg aacgcaatca
      961 aggggccgaa taaggaatca tcggtgaccc cgatcatgac ctccacgccg ccggtcgcca
     1021 tcggctgcac cagcacgcct tccatggcgt cgagctggtt ggtttcggcg agtcgcgttc
     1081 tcatcgagtc aaacgccttg cgaaccgctt gcacatctct gagattcagt tgcacgccac
     1141 cgacctcggt cttgtgcaca atctggcgtg aggctagttt gacggcgact ggatagccga
     1201 cttgcgtcgc caaggccgct gcttcgtcgg ctgttttcgc cacaccaccg gcctgaacag
     1261 gcaccttgat cgcactcagc acatttcgag tttcttccgt cgataaccag ccggcgccgc
     1321 agcgcgcgca agcgtttgca cagatgttcc gtacggctga cagatccaga tcatcgaagt
     1381 caggaaccat gccgggccgt tggtgccgcc actccgcata agccgcagct ttgctcagta
     1441 cgagcgcggg agtctccggc aagtggtaag tcgggatggt ttcagtttgg cagtgaaacg
     1501 t
//
LOCUS       DATMTI010001075         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_294557, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001075 DATMTI010000000
VERSION     DATMTI010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294557"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2061
                     /locus_tag="VN039_17035"
     CDS             <1..>2061
                     /locus_tag="VN039_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWV47705.1"
                     /translation="HAEHVKRITIQIHAASNLDHILLDLHKDILSLFDAEDLTLFAFD
                     SEKKEIFSKLPHIDSVEEVRIPITEQSLAGFCAKYLRPVNIADAYNIAELRAIHPSLL
                     HDTSYDKRTGFKTKQVLTYPIVADNKYLMGVLQLLNKKGGSRFTRRDEEAVDEIAKAL
                     GIAFFNLRKISKKNPTKFDLLVGTNRITQNELDQVIADSRKGMSDLESILIEKYKVPK
                     LDIGRSLAQFYKCPYIEYSERTLVDVELLKNLNVDYLKKNHWMPLKRDRMAIEILTDD
                     PGDLDRVQDIKRTFPGLNIRFAVSLRRDIAQFLSSATGQSDGGGGRKLDENVSDILGE
                     LVTEAQAEAMEDSAAGGGLDENDSAIVRLANQIIADAYRQNASDIHIEPYGEKRETLV
                     RFRVDGDCFEYMKIPQSYRRAILSRLKIMASLDIAERRKPQDGKIKFKLSETKEIELR
                     VATLPTAGYNEDVVMRILAASEPLPLDKMGFSERNLKVLKEISEKPYGIILCVGPTGS
                     GKTTTLHSVLGNINTPDIKIWTAEDPVEITQYGLRQVQVQPKIGLTFANAMRAFLRAD
                     PDVIMVGEMRDKETADTGIEASLTGHLVLSTLHTNSAVETVTRLLDMGCDPFSFADAM
                     LGVLAQRLARRICKDCKEQYIGSKEEYEELRSGYGSEYWDKLGIKQDNTFRLSRGKGC
                     ETCNR"
BASE COUNT          545 a          577 c          536 g          403 t
ORIGIN      
        1 acacgcggag cacgtcaaac gcattaccat ccagattcat gcggccagca atctcgacca
       61 catacttctc gacctgcata aagacatctt aagtctgttc gacgcagaag atctgacgct
      121 ttttgcgttt gactccgaaa agaaggaaat cttctccaag cttcctcata tcgacagcgt
      181 tgaagaagtc cgcatcccta ttaccgaaca gagtctcgcc gggttttgcg ccaaatacct
      241 ccgcccagtc aatattgccg atgcctacaa catcgccgaa ctccgtgcga tccatccgtc
      301 gctgctccac gacacgtcct acgataaacg caccggcttc aaaaccaaac aagtcctgac
      361 ctaccccatc gtcgccgaca acaaatacct gatgggagtc ctccaacttc tcaataaaaa
      421 aggcggaagt cggtttacca gaagggacga agaagcggtc gacgaaatcg ccaaagcgct
      481 gggaatcgct tttttcaacc tccgaaagat atccaaaaag aatcccacga agtttgacct
      541 tctggtcggg accaatcgga tcacgcagaa tgaactcgac caggtcatcg ccgactcccg
      601 aaaagggatg agcgacctgg aaagcattct gatcgaaaaa tacaaggtcc ccaaactcga
      661 tatcggtcga tcgctcgccc agttctataa gtgtccgtat atcgaataca gtgaacgcac
      721 cctcgtcgat gtggaactgc tgaagaacct caatgtcgac tacctgaaaa agaatcattg
      781 gatgccgctc aagcgagacc gcatggcgat cgagattctg accgacgatc cgggtgatct
      841 tgatcgcgtt caagacatca agcggacgtt tccgggcctc aacattcggt tcgctgtcag
      901 tcttcgccgc gatatcgccc aatttctgag ttccgcgacc ggacagagcg acggcggcgg
      961 agggcgcaag ctcgacgaaa atgtttccga tattctcggc gaactggtca ccgaggcaca
     1021 ggccgaggcg atggaagact ccgctgcggg gggaggattg gatgaaaacg acagcgcgat
     1081 cgtccgtctg gccaaccaaa tcattgccga cgcttatcgc caaaatgcat cggacattca
     1141 catcgagccg tacggagaaa aacgcgaaac cctggtccgt ttccgagtcg acggtgactg
     1201 tttcgagtac atgaaaatcc cgcagagtta ccggcgagcc atcctgtccc gactcaagat
     1261 catggccagc ttggacattg ccgaacgccg caaacctcaa gacgggaaga tcaaattcaa
     1321 actctcggag acgaaagaga tcgagcttcg ggttgcgacc ctccccactg ccggatacaa
     1381 cgaagacgtg gtcatgcgta tcctcgcggc gagcgaaccg ttgcctctcg acaagatggg
     1441 gttctcggaa cggaacctca aggtgctcaa ggagatttcg gaaaaaccct acggcatcat
     1501 tctgtgtgtc gggccgaccg gttcagggaa aacgaccacg ctgcactcag tcctgggaaa
     1561 catcaacacc ccggacatca aaatatggac ggccgaggat ccggtcgaaa ttacgcagta
     1621 cggcctgcgg caggtacagg tccagccgaa gatcggcctg acattcgcca atgcgatgcg
     1681 cgcatttctt cgggccgacc ccgatgtcat catggtggga gaaatgcgcg ataaagagac
     1741 ggccgacacc ggcatcgaag cgtcgcttac cggccatctg gtgttgagca cactgcatac
     1801 taatagcgcg gttgaaacgg ttacccgcct gctcgacatg ggttgtgatc cgttcagttt
     1861 tgccgatgcc atgctgggtg tgctcgcaca acgattggct cgccggatct gcaaagactg
     1921 taaggagcag tacatcggaa gcaaagagga gtatgaggaa cttcgatcag ggtatgggtc
     1981 tgagtattgg gataagctcg gcatcaagca agacaatacg ttcaggctgt cccgtggaaa
     2041 gggctgcgaa acatgcaacc g
//
LOCUS       DATMTI010001076         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_82227, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001076 DATMTI010000000
VERSION     DATMTI010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82227"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VN039_17040"
     CDS             complement(<1..1490)
                     /locus_tag="VN039_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47706.1"
                     /translation="MSKSYTAFSQSVMHQLIADQALEKARQIGALSKAPRPGWVRFAD
                     DTGKSTLAPYLPNDVYMHPALAHQLQVVDAYSRNLLERKGPVWEFVRDYYQPMLDMWK
                     YGMTLPNPTHHFRNLWSDMSLTFLAQGAKGFRSAHNKALQVMVTHDGYDGYNFAKAIQ
                     GLGQDIKPGKVLTKSKFGDIGVNDYYSAAMDRGNLPTFRQLEQLGFEGEEGAGKLATK
                     WHSFTNTKAVKKLGSVSEARDHYVRMAHFIQYVEQHANDRKIKGLEELFDKASEQVRK
                     WHPDGSDLTNAERTFKLIIPFYSWTRHAIPLITEAVLTHPARVTAFNKASYALATTMG
                     VNPNSLSDPWPADQTFPSYLTDQEGGPQFNIGGALFKINPGIAPWDVLNQTVGGNPLQ
                     NVVGQATPALRMPFEVATGTNAGTGGPILDWSDYADSQIPGIGQLSRVTGVSPTGTLK
                     AILTGNGQIPQLQRQVAKGNKSPATGALNWLFGLGAQQVNTPSQIRS"
BASE COUNT          336 a          403 c          410 g          361 t
ORIGIN      
        1 gatctaatct ggcttggggt gttcacctgt tgagcaccta gcccgaatag ccagtttaga
       61 gcacccgtag cgggagactt gttacctttc gccacctgtc gctgcaactg tggaatttga
      121 ccgttgcctg tcaaaatcgc cttcagcgtg ccagtgggcg aaacacctgt cacacgggag
      181 agctgaccga tgcctggaat ctgggagtcg gcatagtcgc tccagtcaag gatcgggcca
      241 cctgtaccgg cgttggtccc cgtagccacc tcaaacggca tgcgcagtgc cggggtagct
      301 tgacccacga cattctgtag cgggttacct ccgaccgtct ggttcagcac gtcccacggc
      361 gcaatgccgg gattgatctt gaacagcgct cccccgatat tgaactgcgg gccaccttcc
      421 tgatcggtga ggtagctcgg gaacgtctga tcggcaggcc aggggtccga cagggagttg
      481 gggttcacgc ccatcgtggt tgctagcgcg tagctcgctt tgttgaacgc cgtcaccctg
      541 gcggggtgtg tgagcacggc ttctgtaatg agcgggatag cgtggcgagt ccacgaatag
      601 aacgggataa tcagcttgaa cgttcgctca gcattggtca ggtccgagcc atctggatgc
      661 catttacgaa cctgctcgct ggccttatcg aacagctctt ctaggccctt gattttccta
      721 tcgttagcgt gctgctctac gtactggata aagtgcgcca tacgcacata gtgatcgcga
      781 gcttcggaaa ctgacccaag ctttttaaca gctttagtgt ttgtaaaact atgccactta
      841 gtagctagtt tgccagcacc ttcctcgcct tcaaaaccga gctgttcaag ttgccggaat
      901 gtaggtaggt taccgcgatc cattgcagcg gaatagtagt catttacccc gatatcaccg
      961 aacttactct tggtaagtac cttacccggc ttgatatcct ggcctagccc ctgaatagcc
     1021 ttcgcaaagt tgtagccatc gtacccatcg tgggtaacca ttacctgcaa tgccttattg
     1081 tgggcagagc ggaatccctt agcaccctga gcgagaaatg tcagggacat atccgaccaa
     1141 aggttgcgga aatggtgcgt agggttggga agcgtcatgc cgtatttcca catgtcgagc
     1201 atgggctggt agtagtccct gacgaactcc cataccggac ctttacgctc cagcaaatta
     1261 cggctataag catccactac ctgaagttga tgagccaaag ccggatgcat gtaaacatcg
     1321 ttaggcaggt aaggcgcaag agtgctttta cctgtatcgt ctgcaaatcg aacccagcca
     1381 gggcggggag ctttcgacag agcgccaatc tgtcgagcct tttcaagagc ttggtccgca
     1441 ataagctgat gcattacaga ttgtgagaat gcagtatagg acttgctcaa aaactcaatg
     1501 gggtcatcgg
//
LOCUS       DATMTI010001077         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_591494, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001077 DATMTI010000000
VERSION     DATMTI010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591494"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VN039_17045"
     CDS             <1..418
                     /locus_tag="VN039_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47707.1"
                     /translation="GGFYGHFTSKDDLVAQTLAHALGADSFEGGGFSDFVRSYLAPRH
                     RDNPGNGCPTAGLAAAIRHQTPAAKAVMAEGLRSQVARIEQALPDGGSAGARRAAIGS
                     WAAMIGAVILARAVDDPDLSDEVLEQTRAWIDANVG"
     gene            complement(748..1323)
                     /locus_tag="VN039_17050"
     CDS             complement(748..1323)
                     /locus_tag="VN039_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV47708.1"
                     /translation="MPSGPAIGLNRVLGLDAVSDEVRNWIQTKELPEHSPGWAKLTRS
                     APFSNLDAPVSERTRKVQGDEALLFGSMMCKGFNFPPSLTALWSAIVGKEGWSCFFAL
                     DDETPIGTGAMYVSGSYAWLGGGTTAPEFRNRGVQKALIQARTDYGIEQGVLTFVVET
                     EVPSAGKLNISYDNLRKIGFQHAYNRKNFKL"
     gene            complement(1637..1873)
                     /locus_tag="VN039_17055"
     CDS             complement(1637..1873)
                     /locus_tag="VN039_17055"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV47709.1"
                     /translation="MSDDEDELFHVVKGSLRIEYEDRFVHLRQGDSHIVPKATMHNPV
                     ADKECWIVLIEPVGTKHTGDATTDRTRSIDEQLQ"
BASE COUNT          444 a          619 c          621 g          470 t
ORIGIN      
        1 tggtggcttt tacggccact tcacctcaaa agacgatctg gtggcgcaga cccttgcgca
       61 cgccctcggc gcagacagtt ttgaaggtgg cgggttcagc gatttcgtcc gctcctatct
      121 ggcgccgcgt caccgggata atcccggtaa tggatgcccg acggcaggcc tggcggctgc
      181 cattcgtcac cagacgcccg cggcgaaagc cgttatggcc gagggactgc gctcccaagt
      241 cgcacgtatt gagcaggcgc ttccggacgg gggttcggcc ggtgcccgcc gcgcggcgat
      301 cgggagctgg gcggccatga tcggagccgt catcctcgct agggcagttg acgatcctga
      361 cttgtctgac gaggttcttg agcagacgcg cgcttggatc gacgcaaatg tcggctagga
      421 gcatcagaga gtgcgcattt caagtgcctg atctacgtct gcttcgagga aacgatcgaa
      481 tttgacgtga cgaccgacat gggggcgcga agcggtaact attccgctct tacgccctaa
      541 gcgggaggcc agcggagtgc tgcatagagg cttgaggcgc aacataagag agatggcggt
      601 tcactatgtc gtcggggaag acctccaggc atcatcacct gcgatgatca ggacgcggtg
      661 tagttgccct caaaccgttg aggaacaggc ttttgtgtga cgatccagaa aatcacccgc
      721 tggcctgaaa gaagatcgcc agatgtgcta aagcttgaag ttcttccggt tgtacgcgtg
      781 ctggaagcct atttttctga ggttgtcata ggagatattg agcttgccag ccgagggtac
      841 ttctgtttcg acgacgaacg tcaaaacgcc ctgttcgata ccgtagtcgg tgcgagcctg
      901 gatgagcgcc ttttgaacgc cccgattccg aaactcgggg gctgtggtac cgccaccgag
      961 ccacgcatat gaacccgaga cgtacatggc gccggtgccg ataggcgttt catcatcaag
     1021 agcgaaaaaa cacgaccagc cctctttacc cacaatggcg gaccagaggg cggtcaagct
     1081 tggcgggaag ttgaaaccct tgcacatcat cgatccgaag agcagggctt cgtcgccttg
     1141 aacttttcgg gtgcgctcac tgaccggcgc atcgaggttg ctaaaaggcg cgctgcgggt
     1201 aagctttgcc catccgggac tgtgctcggg cagctccttg gtctggatcc agtttctcac
     1261 ctcgtccgag acagcgtcca gccccagaac acgatttagg ccgatcgccg gtccgcttgg
     1321 caacgagatc gcgcatccgc cgttgatgac aaggcaggtc gctgtctggt cagcacactg
     1381 aagtcgccat cggttgcgcc gtcctcaacc gcatgcttgc ttgcgcacgc ccgaaagccg
     1441 tccgccgcaa agcagccacg ccgtagtcag ccgtttcaaa gatccccatc cgttcagttc
     1501 cacatccgtg caccaacgcc cccgtcgtgc tcgaaacgtg gtcggccgag aagctcggaa
     1561 aagacggctt gaccttgctg cttataaaaa acgtcggcgt ctcagtttgc ttccttttgg
     1621 ttgagaacaa agaaggtcat tgcaactgtt cgtcgatgct gcgggtacgg tcagtggtcg
     1681 catcaccggt gtgcttcgtg ccgacgggtt cgattaggac gatccagcat tccttgtctg
     1741 ccaccggatt gtgcatggtc gccttcggca cgatatggga atcgccctgc ctcaggtgaa
     1801 caaaccggtc ttcgtactcg atgcgaagag atcctttgac gacatggaag agttcgtcct
     1861 cgtcgtcgct tatgccaaac tagctctcca cgaaatttgg cgaccttcag ttattggtca
     1921 ttaacgcagc caatgacctt gggagaccaa tgctctgtca cgctctggag cgctgtaacg
     1981 aagttatcgg atgcatgcac tgcaatctcc ctttcgttcg tgtttgagtc agaagcctcg
     2041 ctgcgggtca atcaacggga ctccacttac tcgatctccg ctaggttcta tggacctgat
     2101 ttcccgcaaa ggcagccgtt attccaaggc caatcaagag gcccccactg acat
//
LOCUS       DATMTI010001078         1833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_337831, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001078 DATMTI010000000
VERSION     DATMTI010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337831"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..916)
                     /locus_tag="VN039_17060"
     CDS             complement(80..916)
                     /locus_tag="VN039_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47710.1"
                     /translation="MLRRAYPLTFLTAFGLLFAACALFAPKHEAPPLKQVTAEELTVL
                     LHQREAAVHSMKGLFSAKVRGGPIPIASRVEGAVYYRRPNALRLRGFTPLGNELFDFV
                     QADDLYELRLPLEGKTYAGRQSDMKNTGKLARFSQLSVWAVGGVLGTNSIATDEMVKV
                     VEEKGRYRLDVYAPATGGTSPSRPPIRRLWFDRRLLVVQEEWYGSSGEVEATIQYDDF
                     RPLEEVGSISAEAMGDQDVRLFRPFKISLEDGRGQGSVLVTFHEMHHNQAIRAEDLGQ
                     IS"
     gene            complement(1134..>1833)
                     /locus_tag="VN039_17065"
     CDS             complement(1134..>1833)
                     /locus_tag="VN039_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HWV47711.1"
                     /translation="GPRLLEHIVDTVLYFEGDKGHSYRILRAVKNRFGSTNEIGVFEM
                     KDVGLEEVNNPSELFLAERPERSTGSVVVSSLEGTRPILVELQALVSSTTYAMPKRMA
                     NGVEVNRVSLLLAVMEKRLGVHLSGQDVYVNVVGGMHIDEPAIDLGIVAAVTSSLREV
                     PVEPGTLLLGEVGLGGEVRAVSQAELRIRESAKMGFKRCILPERNLAKLDPIDGMELI
                     GIQEVREALDVVLA"
BASE COUNT          414 a          574 c          430 g          415 t
ORIGIN      
        1 ctaggatcag gtcgtcttcg agaatcgcga cactctgcca cgtcgtagcc gtttcaaccg
       61 caaggatttt catcgataat tatgaaatct gacctaagtc ttctgctcgt atggcttgat
      121 tgtgatgcat ttcgtggaag gtgactagca ctgagccttg tccacgacca tcttccagag
      181 aaatcttgaa ggggcggaat agccgaacat cctgatcacc catggcctct gctgagatac
      241 ttcccacctc ttcgagcggc ctaaaatcgt catactgaat cgtcgcctcg acctccccgc
      301 tcgacccata ccactcctct tgtaccacca gcaatcggcg gtcaaaccat aggcggcgga
      361 tcggaggacg cgatgggctc gttccccccg ttgccggcgc atagacgtcg agacgatatc
      421 ggcctttctc ttccacgacc ttcaccattt catccgtggc gattgagttc gtccccaaca
      481 cgccaccgac cgcccataca ctcaactgag agaatcgagc cagcttccca gtatttttca
      541 tatcggactg gcgaccggca taggtttttc cttcaagagg caagcggagc tcgtacagat
      601 catctgcttg aacaaagtcg aacaactcgt ttccaagagg agtaaaccct ctcagccgca
      661 acgcgttcgg ccgacggtag tagaccgcgc cctcgactcg tgaggcaatt ggaatgggcc
      721 caccccgcac tttcgcgctg aacaaacctt tcatggaatg aacggccgct tctcgctgat
      781 gcaacagcac ggtcagttct tcggcggtca cctgcttaag cggcggcgct tcatgcttcg
      841 gcgcaaatag cgcgcatgcg gcgaagagga gcccaaacgc cgttaaaaat gtaaggggat
      901 aggcacgtct cagcatccta tttcctcaat cccctatggc atggcggagt ggaagggtct
      961 cttactgcgc gctaatgttt cacccaccca accctcgacg cgccaaggcg cgtctttccc
     1021 agagtgaggg ctttccgcgc ggtaacggtg cgtctcggcg catcggggga ggcgggtgag
     1081 ataaacgcgc gttgggcgag cacagagacc cttccgcttc gccatgcctc tcttcacgcc
     1141 aacaccacat ccaaagcctc ccgcacttcc tggataccaa tcagctccat accgtcgatg
     1201 ggatcgagct tcgccaaatt tcgttcaggc agaatgcatc gcttgaaccc catcttggcg
     1261 gactcgcgaa tacgcaattc agcctgacta accgcacgta cctcaccccc caaacctact
     1321 tctcccaata gtaacgtccc cggctcgaca ggcacctccc gtaaactcga tgtgacagct
     1381 gcgacaatcc ccaagtcaat agccggttcg tcgatatgca tgcctccaac gacattcaca
     1441 tacacatcct gtcctgaaag atgcactccg agtcgtttct ccatcacggc gagcagcagc
     1501 gagactcgat tgacctccac tccgtttgcc attcgtttgg gcattgcata ggtggttgag
     1561 gaaaccaacg cttgcagttc aaccaggatc ggtctcgtac cttcgagact cgataccacc
     1621 acagatccgg tgctccgttc agggcgttcc gccagaaaca attcagatgg gttattcacc
     1681 tcttcgagcc ctacatcttt catttcgaat actccgattt cattcgtcga tccaaaacga
     1741 ttcttcacgg cgcggagaat tcggtagctg tgtcctttat ctccttcaaa gtataaaacc
     1801 gtatcgacga tatgttccag cagccgtggt ccg
//
LOCUS       DATMTI010001079         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_591553, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001079 DATMTI010000000
VERSION     DATMTI010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591553"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1379
                     /locus_tag="VN039_17070"
     CDS             189..1379
                     /locus_tag="VN039_17070"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV47712.1"
                     /translation="MPIDEITQKVSDRYAKAASTGEQMCCPTSYDMGHLKTFIPEEVL
                     KISYGCGTPAGLKTVQAGETVLDIGSGGGIDCFEASRLVGHTGRVIGIDMTDTMLEIA
                     RKNASIVAANLGYPTSNVEFRKGLADAMPVDDGTIDLIISNCVINLAPDKRKVFQEMY
                     RVAKPGGRFTISDIVSDHIVPQYLVHDTRKWGDCLSGALTLTDYMNGMTAVGFLGIHL
                     IKFSPWRVIDGIHFFSVTLTGYKLPPALTASSIRYATLRGPFSRLVDERGTKYQRGIP
                     QPISTDDVTLLSHPPFADHFLLTADPVALDNHDPRWTAVFPAQAPCTWQGHYALLAGP
                     FIEAADDDHHIYRRGEPLEICSKTLVVLENEGYEPHFVVLNRAGDRVSGEAVTCSPNE
                     GGCC"
     gene            1379..1735
                     /locus_tag="VN039_17075"
     CDS             1379..1735
                     /locus_tag="VN039_17075"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWV47713.1"
                     /translation="MKPPLNAKMLTPRQCAMVLKALGDETRLRILESLLIGEKCVTDL
                     VQELRCSQPHASHHLRILRDSGLIEGQRDGKQVCYKVSPAIQRALGNRQGQALDFGCC
                     ELRFPETVLMTIGHAR"
     gene            1783..2820
                     /gene="arsS"
                     /locus_tag="VN039_17080"
     CDS             1783..2820
                     /gene="arsS"
                     /locus_tag="VN039_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HWV47714.1"
                     /translation="MALTLLGRHNPLASPSEQLKVLAQSGPRLSFEAQLNQAGLYPLR
                     AVEITVFQINVGKLCNQTCRHCHVDAGPDRPETMSLETAEQCVRALAKTDIPTVDITG
                     GAPELNPHFRWLVEQIRGLDRHVIDRCNLSVLLLPSQADLAEFLASHRVEIIASLPSY
                     RAGQTDAQRGDGVFEKSMEALRLLNRFGYGRPDGGLTLNLVYNPVGAFLPPKQDAIEA
                     QFKKELRTRHGIEFNRLYTITNMPISRFLEFLVESGNYEQYMTRLANAFNPAAAAGVM
                     CRYTLSVGWDGRLYDCDFNQMLDLPLDHRASSHVRDFDPAQLHDRQIVTRNHCYGCTA
                     GSGSSCGGSVT"
     gene            2958..>3218
                     /locus_tag="VN039_17085"
     CDS             2958..>3218
                     /locus_tag="VN039_17085"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWV47715.1"
                     /translation="MPAADQLSGKLVITGASTLAPLIGEIGKRFESLYSKVRVDVQTG
                     GSSRGVADVRQGLADIGMVSRVMKDDEKDLHAFPVARDGVGII"
BASE COUNT          705 a          945 c          835 g          733 t
ORIGIN      
        1 gtaagattta ctccctgaga cagacatcat ttaatggacc acccaccgga ttgctagccc
       61 acgtgaatgg atttgctcct ttcgtccatc atgcaatcag aatgtcccaa ttgacgcgtg
      121 cataaaggat tggtacatag agttagctct atatctatac tttgccagac caacgcttga
      181 ggccggccat gcctattgat gaaatcacac aaaaagtcag cgatcggtac gcgaaagccg
      241 ccagtaccgg cgagcagatg tgttgcccca ccagctacga catggggcac ctcaaaacct
      301 tcattcccga agaagttcta aaaatctcct atggctgtgg cacaccggcc ggcctcaaga
      361 cggtgcaggc cggtgagacg gtccttgaca tcggttctgg cggcggcatc gactgttttg
      421 aagcctcccg cctcgtcggc cacacaggac gcgtgattgg gatcgacatg accgatacga
      481 tgctggagat cgcccgcaag aatgcatcaa tagtggctgc gaacctcggt tatcccacat
      541 cgaacgtgga atttcgcaaa ggcctggccg atgcgatgcc ggttgacgac gggacgatcg
      601 atttaatcat ctcgaattgc gtgatcaacc tcgcccccga caaacggaag gtgtttcagg
      661 aaatgtatcg ggtcgccaaa cccggcggac ggttcaccat ttccgatatt gtatcggacc
      721 acatcgtccc acaatatctc gtccacgata cccggaaatg gggagattgt ttgtccggag
      781 ccttgacgct taccgactac atgaacggca tgacggcagt cggatttctt ggcatccatc
      841 tcatcaagtt ttcaccctgg cgcgtcatcg acggtatcca tttcttttcc gtcacattga
      901 cgggttacaa gctccctccg gcactgacgg cctcttcgat ccgctacgcc acccttcggg
      961 ggcctttcag ccgcctcgtg gacgaacgag ggacaaaata ccagcgtggt attccgcaac
     1021 caatcagcac ggacgatgtc acgctgctga gccatcctcc attcgccgat cattttctcc
     1081 tgactgccga ccctgttgca ctggataacc atgatccccg ctggactgcc gtgtttccgg
     1141 cacaagcccc ttgcacctgg caaggccatt acgcgctgct ggccggaccc ttcatcgagg
     1201 cggccgacga tgaccaccat atctatcgcc gtggcgaacc actggaaatc tgttccaaga
     1261 cattggtcgt gctggagaat gagggatacg aacctcattt tgtcgttctc aatcgtgccg
     1321 gcgatcgtgt gagcggtgaa gccgtaacct gctcccccaa cgaagggggt tgttgctgat
     1381 gaaacctcca ttaaatgcca agatgctgac cccgcgccaa tgcgccatgg ttctcaaggc
     1441 gttgggcgat gaaacgcggc tgcggatatt ggaatccttg cttatcggtg aaaaatgcgt
     1501 gacggacctc gtgcaagaac tccggtgctc tcagccccac gcctcccatc atctccgtat
     1561 cttgcgtgat tccggcttga tcgagggaca gcgagacggc aaacaggttt gttacaaagt
     1621 ctctcccgcg attcaacgag cgctgggaaa tagacaagga caagccctgg atttcggctg
     1681 ctgtgagttg cgatttccgg agacggtgtt gatgacgatc ggtcatgcgc gctgaagcag
     1741 accgtattct gagaagcctt ccccattctg cagagcacag atatggcgct gaccctactg
     1801 ggacgacaca atcccctcgc ttctccttct gaacaactca aggtgttggc ccagtccggc
     1861 ccccgccttt cctttgaagc gcaactgaat caggccggct tatatccgct acgtgccgtt
     1921 gaaatcaccg ttttccagat taatgtcggc aaactgtgca accagacttg ccgtcactgt
     1981 catgtggatg ccggtccgga tcgtcccgag accatgtcct tagaaacggc tgaacagtgc
     2041 gttcgagctc tggcaaaaac cgatattccc acagtcgata ttacgggcgg ggccccggaa
     2101 ctcaatccgc actttcgttg gttggtcgaa cagatccgtg gtctcgaccg gcatgtcatc
     2161 gatcgctgca acctgtccgt attgctgctg ccctcgcagg ctgacttggc ggaatttctg
     2221 gccagccatc gtgtcgagat catcgcatcg cttccctcgt atcgtgccgg ccagaccgat
     2281 gcgcagcgag gcgacggcgt ctttgaaaaa tcgatggagg cactccgtct cctgaaccgt
     2341 ttcggctatg gacgaccgga cggcgggctc accctgaacc ttgtctacaa tcccgttggc
     2401 gcattcctcc ctccgaagca agatgccata gaagcacagt tcaagaaaga actgcgcact
     2461 cgccatggca tcgagttcaa ccggctctac accatcacca acatgccgat cagccgattc
     2521 ttggagttcc tcgtcgaaag cggcaactat gaacaataca tgacgcgctt ggccaacgca
     2581 ttcaatcctg ccgccgccgc cggtgtcatg tgccgctata ctctgtcggt tggatgggac
     2641 ggcaggctgt atgactgcga cttcaaccag atgctcgacc tacccctcga tcatagagcg
     2701 tcgtcgcatg ttcgagactt cgatcccgcg caacttcatg atcgtcaaat tgtgacacgt
     2761 aaccactgtt acggctgcac ggcaggttcc ggctcatcct gtggtggttc ggtcacttga
     2821 ataatcgcct tcgactcata tatgtagtcg tctacatgta tcgatttatt tgttcatgtg
     2881 gatggagaac gtgtgcttcg ctcgcctcat tcttaattgt cgcggtcttc gggattccgg
     2941 aatcagtgac agcccaaatg cccgcagcag atcaactgtc cggcaaactg gtgatcaccg
     3001 gtgccagtac cctcgctcca ctgatcggcg agatcggcaa acgattcgag agtctctact
     3061 ccaaagtacg tgtcgatgtg cagaccggcg gctcatcacg cggagtcgcc gacgtgcgtc
     3121 agggactcgc cgatatcggc atggtctcgc gcgtgatgaa agacgacgaa aaagatctgc
     3181 atgcgtttcc ggtggcgcgc gacggcgttg ggatcatt
//
LOCUS       DATMTI010001080         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_634194, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001080 DATMTI010000000
VERSION     DATMTI010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634194"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VN039_17090"
     CDS             complement(<1..271)
                     /locus_tag="VN039_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47716.1"
                     /translation="MSALAGLIGKQRPPVQGNVISKMLAAMSRESDTTLNTRFLPGAG
                     LELGWLSASGSNNECTFVSDRENTLFLLLRGELFSGDEIGTNGSCT"
     gene            complement(277..1200)
                     /locus_tag="VN039_17095"
     CDS             complement(277..1200)
                     /locus_tag="VN039_17095"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWV47717.1"
                     /translation="MRCGSTWLYQVLRCHPDIQLATCKEMDFFFMRKMLKHDLDWYQN
                     HFVTPAHAGPKPIRGEISPLYARLKSWQVKRIAALLPKARIVLTLRHPIERVWSQAVY
                     EFGHRSHRDVRKVRAVEFLRQVERQRSRLSSDYCRMIKVWSDAFGSDALYVGLFDLLR
                     DDPQTFINPILKHIGASTPWLLPSEFVQKRVWATKTLVQHDREIPDLVRWYIADRELE
                     PTQRLNELLEGRVSHWVNELRDIRGETPLRWRMLKHLNRSVLSVPETLAYEAYHVMLD
                     LRLLLRWQHLRSIYRFERDIDSPNLNLQNSY"
     gene            complement(1333..1404)
                     /locus_tag="VN039_17100"
     tRNA            complement(1333..1404)
                     /locus_tag="VN039_17100"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1370..1372),aa:Thr,seq:tgt)
     gene            complement(1521..1609)
                     /locus_tag="VN039_17105"
                     /pseudo
     tRNA            complement(join(1521..1569,1573..1609))
                     /locus_tag="VN039_17105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /pseudo
                     /anticodon=(pos:complement(1574..1576),aa:Ser,seq:cga)
     gene            complement(1632..>2136)
                     /locus_tag="VN039_17110"
     CDS             complement(1632..>2136)
                     /locus_tag="VN039_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47718.1"
                     /translation="FNYYGYDAPTIHMDLQSIVDRSYFIGAYHGDELLGFAKLFHGNG
                     VLRTVHIIAKLAHRDKPVQDALIERAVQICEEKGIEYLQYGSWSEGGLGSFKMKHGFR
                     KFDYSRYYVPLTWRGRVALRFRLHHGVKGWLSEDWVSSLRNIRNTWNHSRYSRLSRMT
                     RPVAHRA"
BASE COUNT          528 a          577 c          526 g          505 t
ORIGIN      
        1 tggtgcaaga gccattcgtt ccgatttcat cacctgaaaa cagttctcct ctcagcagca
       61 gaaacagtgt gttttcccga tccgaaacaa aggtgcattc gttgttcgat ccagacgccg
      121 acaaccaccc cagttctaat ccagcaccag gaagaaatcg cgtgttaaga gtcgtgtcgg
      181 attcacgcga catcgcggcc agcattttcg agatgacgtt cccttgtacc ggaggccgct
      241 gtttccctat caatccagct agtgctgaca tcctggctag tatgaattct gcaggttcaa
      301 attcgggcta tcgatgtccc gttcaaagcg ataaatgctg cgtaaatgct gccatcgtag
      361 taacagcctt aagtcaagca tcacatggta tgcttcgtag gccagcgttt ccggtacgga
      421 tagcacactg cgattcaagt gctttaacat tcgccaccga agaggcgtct cgccacgaat
      481 atcgcgaagt tcattcaccc aatgcgaaac tcgaccctca aggagctcgt tcagccgctg
      541 agtgggctcg agctcgcggt ccgcgatata ccaacgtacc aggtccggaa tttctcgatc
      601 gtgctgcacc aaagtcttcg ttgcccagac gcgcttttga acaaattctg atggcagcaa
      661 ccatggcgtg gatgcgccga tgtgcttcaa gattggattg ataaaggttt gcggatcgtc
      721 gcgtaacagg tcaaacaatc cgacgtatag cgcgtccgat ccaaacgcat cggaccaaac
      781 tttaatcatc cggcagtaat ccgacgacaa tctgctgcgt tgacgttcga cctgccgaag
      841 aaattcaacg gcccgcactt tccgcacgtc ccggtgactt cgatgcccga attcgtacac
      901 tgcttgcgac cacacccgtt cgatcggatg ccgcagcgta aggacgatcc gagccttcgg
      961 gagtaaggca gcaatgcgct taacctgcca gctcttcaag cgagcgtata aaggtgagat
     1021 ctcgccgcga atcggtttcg gcccggcgtg ggccggtgtc acaaaatggt tctgatacca
     1081 atccaggtca tgcttcagca ttttgcgcat gaagaaaaag tccatctctt tgcacgtggc
     1141 taactggatg tcggggtgac acctaagcac ctggtacaac catgtggaac cgcaccgcat
     1201 cgagccgatg ccgaggaagg ttggtaaatt gcgcggtcgc gccaaaacgc ttgaagagag
     1261 tctgttcacg acgattgcga cctcgttttt ggctcggagt aacaactgct gcacgcaatc
     1321 aaaatgatct tgagccggcg ggaggaattg aacactccat ctgctgatta cagatcagct
     1381 gctctaggct tgagctacac cggcaattga caacctgaga gaattgcctt tgccatgagc
     1441 tgccgatcct gaagcacatc aaatacgctt ctttgattgt gcttaactta caaagctcta
     1501 acaattatgt ttcaatatac cggagagggg aaggattcga acctccgatg cgagttatcg
     1561 catagcctcc ttttcggaat gctgccttag gccactcagc cacctctcct ctgtaacaaa
     1621 tcattttggt cttaggcgcg atgcgccaca gggcgagtca tgcgcgaaag cctgctgtat
     1681 cgagaatggt tccaagtatt tcggatattt ctcaaggaac tgacccagtc ctccgataac
     1741 catcctttga cgccgtgatg tagccgaaat cgcaatgcaa cacgcccgcg ccaggtcagt
     1801 gggacgtagt aacgcgaata atcgaatttc ctgaagccat gcttcatttt gaatgagccc
     1861 aagccaccct cactccatga gccatattgc aaatactcga tgcctttctc ttcgcagatc
     1921 tgaacggcgc gctcaatcag ggcgtcctga accggtttat cgcgatgcgc cagtttggca
     1981 atgatatgga ccgtgcgcag aacaccattt ccgtggaaga gctttgcaaa tcccaaaagt
     2041 tcatctccat ggtatgcgcc gatgaaataa gatcgatcga cgatggattg caggtccatg
     2101 tggatggtgg gtgcatcgta accatagtag ttgaac
//
LOCUS       DATMTI010001081         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_805190, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001081 DATMTI010000000
VERSION     DATMTI010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805190"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VN039_17115"
     CDS             <1..218
                     /locus_tag="VN039_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47719.1"
                     /translation="ETCAVEAGNGISALNTEEGIMFVRLLAPGCWALTADHRVFYIHR
                     RHSRRAHLRGMRIFDCRDPWSPDKGIA"
     gene            375..800
                     /locus_tag="VN039_17120"
     CDS             375..800
                     /locus_tag="VN039_17120"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HWV47720.1"
                     /translation="MVRARSDKSKGKPSVGKEKARAGRSDEGEATNAESAGRGLSKNS
                     AQRNSLPPVAANPQKRQEHQRMEEEPSADLQESEKFSEEALSIIASPDGHQDSEDHPA
                     THRRIAEQAFTLFQESGCEHGNDWAHWFEAERQIKETQM"
     gene            844..1227
                     /locus_tag="VN039_17125"
     CDS             844..1227
                     /locus_tag="VN039_17125"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWV47721.1"
                     /translation="MHNRNYYDEGIEDATGWSAFVAGALIGAGIALLFAPQPGTELRG
                     MLRSYANRAKDDLIDRGQEAWDTAVERGREYYEKGEEVVRDAGRSAREFAKQGQEAVK
                     EAGRSAKDFAKQAQDVAREPGRSAL"
     gene            1354..1650
                     /locus_tag="VN039_17130"
     CDS             1354..1650
                     /locus_tag="VN039_17130"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWV47722.1"
                     /translation="MACQAPISQAPTGKTALESLNDDSIKASVQGKLAADNVQDMNVV
                     AFANVVVQAERGAVILSGVVQTSEQKARAEQLAGEVNGVTHVTNNLQVRTGKKR"
BASE COUNT          454 a          385 c          538 g          332 t
ORIGIN      
        1 atgaaacctg tgccgtggaa gcgggaaatg gaataagcgc tttaaatacg gaggagggca
       61 tcatgtttgt acgattgctg gccccgggct gttgggctct gacagctgat catcgcgtgt
      121 tctacattca ccgaaggcac agtcgccggg cgcatcttcg tggtatgcgc atcttcgatt
      181 gtcgtgaccc atggtcgccc gacaaaggaa tcgcgtagct ggaggaatgg gcagtaggtg
      241 accacgtccg agtcgaagcc tccgacatgg gtactgcgac gacgatcaag cagttgctgt
      301 cgttgcgaag cggagaatag tcaaacgtcc aagatgaagg atttcgtcaa ttatcaacgg
      361 atgggggaga ggagatggta cgagcacgtt cggacaagag taaaggcaag ccatcggtcg
      421 gtaaagagaa agcgagagcc ggtcggtcgg atgaggggga ggcaacaaat gcggaatcgg
      481 caggacgggg tttaagcaaa aactccgccc aacgcaattc cttgccgcca gtcgccgcta
      541 acccccaaaa gcggcaagag catcagcgga tggaagagga gccgtctgct gatttgcaag
      601 aatctgaaaa gttttccgag gaagcgcttt cgattattgc ttctcctgat ggacatcagg
      661 atagcgagga tcatccggcg acgcatcgcc gaatcgcgga gcaggcgttt actctgtttc
      721 aagagagcgg ctgcgaacat ggcaacgatt gggcccactg gtttgaagcg gaacggcaga
      781 tcaaagaaac tcaaatgtaa tctcaaaccg tatttctcag cgtcaaaaaa ggagggcggt
      841 attatgcaca acaggaacta ttacgatgaa ggaattgagg atgccacggg atggtccgct
      901 tttgtggccg gagcgttgat cggagccggc atcgctctgc tgtttgctcc tcagccgggc
      961 acagagctgc gcggcatgtt gcgcagttat gccaatcgcg cgaaagatga tctgatcgat
     1021 agggggcaag aggcatggga taccgcagtg gagcgaggca gagaatatta tgagaaggga
     1081 gaagaagttg tccgggatgc cggacgatca gccagggaat ttgcaaaaca gggacaggag
     1141 gcggtgaagg aggccggacg gtcggcaaag gactttgcca aacaagccca agacgtggca
     1201 cgtgagccgg gacgttcagc tctgtaaagg atgcaaggac taattcacaa ggaatgatta
     1261 tcaagggaat aactcatgag tagaaacctt atgagaaggt cgtcgtttgt tctcgtactt
     1321 tttgttctcg tgctaagcag ggtgagcgga gtcatggcct gtcaggctcc gatcagtcag
     1381 gctccgaccg gcaaaaccgc cttagaaagc ttgaacgatg attcaatcaa ggcgtccgta
     1441 caggggaaac tggcggccga taatgttcaa gatatgaatg tggtggcctt cgcgaatgtc
     1501 gttgtgcagg cagaacgagg ggccgtgatt ctcagtgggg tggtgcaaac ctcagagcag
     1561 aaagctcgtg cggaacagct ggccggagag gtaaacggcg tcacgcacgt caccaacaat
     1621 ctccaagtca ggacgggtaa gaaacggtga gcgcattcgg caagatcatg agacagcccg
     1681 atgtcgcgac agaaattagg ctctccacc
//
LOCUS       DATMTI010001082         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_294718, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001082 DATMTI010000000
VERSION     DATMTI010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294718"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VN039_17135"
     CDS             complement(<1..381)
                     /locus_tag="VN039_17135"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWV47723.1"
                     /translation="MARIQTPFNLETFLGKVDKGKTILTSPKKQILFSQGDTTDAVFY
                     IQAGRVKLTVVSEQGKEAVVAILERGAFCGESCLVGQTVRTATATSLEDSRILRIEKA
                     VMFRMLQEQPRFSEAFMSYLLVHSI"
     gene            complement(553..765)
                     /locus_tag="VN039_17140"
     CDS             complement(553..765)
                     /locus_tag="VN039_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47724.1"
                     /translation="MKTTPRTYVLTLNSPSRKGLRLWMGDSIHALRHCSVKQIGLALR
                     EMQQTVDRLQTSVSSSGGSYSLNRGR"
     gene            complement(896..1090)
                     /locus_tag="VN039_17145"
     CDS             complement(896..1090)
                     /locus_tag="VN039_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47725.1"
                     /translation="MTTEEHISKMKKAITQAIKVMGDRFGSTEQDVARAIQELKASMQ
                     GVSEPSLSVKTVPGPGSGPC"
BASE COUNT          354 a          459 c          370 g          345 t
ORIGIN      
        1 gatcgagtgc accagtaaat aggacataaa tgcctcggaa aacctgggct gctcctgaag
       61 catgcgaaac atgacggctt tctcgatgcg gagaatgcgg gagtcttcca ggctggtcgc
      121 ggtcgccgta cggaccgtct ggcccacgag gcaggattct ccacaaaaag caccccgctc
      181 aagaatcgcc acgacggctt ccttgccctg ttccgagacc acggtcagtt tcaccctgcc
      241 cgcttggata tagaacaccg catcggtcgt atccccttgt gaaaagagaa tctgtttctt
      301 gggggacgtg agaatcgttt tgcccttgtc gaccttaccg aggaatgttt ccagattgaa
      361 cggtgtttgt atgcgtgcca tatatcacca tcctctactt agagcgactg aaccaaccac
      421 ttacgtgaca gaacacctta gtcgtaccga aagacgttgg ccatgcatca gggcccatac
      481 acatggctcg tgtccgctcc cgacacggtc tctcccgccc ccaatctcta ctcactcgcc
      541 gaggacatca cgttatcgac cgcggttcaa cgaataggat ccgcccgacg acgagacgga
      601 ggtctgcagg cgatccacag tctgttgcat ttccctcaat gccagaccga tctgtttgac
      661 cgagcaatgg cgcagggcat ggatactgtc ccccatccac agccgaaggc cttttcggga
      721 cggtgaattc agcgtcaaca catacgtccg tggtgtggtc ttcatactgt cctcctagat
      781 attatgcccc ccgcttcacg ctcgagtcga gcctgaaaca acgggccatg ttcggtttcc
      841 tatcatgccg cccgaaatac tgcgagcaga tcactcaatg ccatggtttt gtgtttcagc
      901 acggcccgct gcctggcccc ggcacggttt tcactgaaag gctcggttcg gacactcctt
      961 gcatcgacgc cttcagttcc tggatggctc ttgcgacatc ctgctccgtc gatccaaacc
     1021 gatctcccat caccttgatg gcctgagtga tcgctttctt catcttcgag atgtgttctt
     1081 ctgttgtcac aaccgctctc tccttcgtga atttccacgg tagacttgat cggtcaggac
     1141 atccggatgg tgtgtcgtcc catcaagtgg atcatcaata atacaatccc actcacaagc
     1201 agaatccagg aactctgagt cgcaaccgtg gctattccag tccaattcaa gcccacggca
     1261 atcaatccga agaataagaa catcgctgcg taggacacca tagtgaattc ccttccaacg
     1321 ttgcgggcac tttaaggaac gtcccctgca catcctggcg tctgccctca cttcaggtaa
     1381 ctacatgaca ttaccctggc aagcgcaacc aagaatcgcg catcgtgaga aacgatcgca
     1441 gtctgaagac actctctggc gtcaaagcgg cggggagcgc aaaggacgag acagccagcg
     1501 tattcaaacg tcagggagga aggaagaa
//
LOCUS       DATMTI010001083         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_591630, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001083 DATMTI010000000
VERSION     DATMTI010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591630"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /gene="rpsM"
                     /locus_tag="VN039_17150"
     CDS             <1..248
                     /gene="rpsM"
                     /locus_tag="VN039_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWV47726.1"
                     /translation="KIVKLREIIERDFRVEGDLRKEVSLNIKRLIDTGTYRGLRHRKG
                     LPVRGQRTKTNARTRKGRRAGVSSKPRPTVTKRASST"
     gene            276..659
                     /gene="rpsK"
                     /locus_tag="VN039_17155"
     CDS             276..659
                     /gene="rpsK"
                     /locus_tag="VN039_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWV47727.1"
                     /translation="MSVKKGKKKERRIVQSGVAHVQASFNNTIVTITDMSGNTVVWAS
                     AGNQGFKGSRKSTPFAAQRAGEAAARKAMESGMRQVDVYVNGPGSGRESAIRSLQGAG
                     LRINLIRDVTPIPHNGCRPPKRRRV"
     gene            746..1372
                     /gene="rpsD"
                     /locus_tag="VN039_17160"
     CDS             746..1372
                     /gene="rpsD"
                     /locus_tag="VN039_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWV47728.1"
                     /translation="MAKYRGPVCRLCRREGEKLFLKGTRCMTEKCAVERRSYPPGQHG
                     QGRQRTSDYSLQLREKQKLRRIYGLQECQFRGVFERAERQTGVTGDALLRLLECRLDN
                     VAYRLGFGASRKQARQMVSHGHFTVNGKKITVAGALVKPGDVIEIRERSRDLAAIQTA
                     LESVDSRGIPDWLELDKGAFKGTVRALPAKEQIALPVNEQMVVELYSR"
     gene            1487..>1768
                     /locus_tag="VN039_17165"
     CDS             1487..>1768
                     /locus_tag="VN039_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWV47729.1"
                     /translation="MRVEVDKDAHSPTFGRFTTEAFERGFGTTIGNALRRILLSSLTG
                     AAVTTVKIEGVVHEFSTISGVTEDVTAIILNVKSLRLALHTDKPKTIRLK"
BASE COUNT          435 a          417 c          545 g          371 t
ORIGIN      
        1 ataagatcgt caagttgcgt gaaatcattg agcgtgattt ccgggtcgaa ggagacctac
       61 gcaaagaagt gtcgctgaat atcaagcggc tgatcgatac ggggacttat cgaggattgc
      121 gtcatcgcaa aggtcttccc gtccgtggac aacgaaccaa gaccaatgcc cggacacgta
      181 aaggacggcg cgcaggcgtc agtagcaagc cgagacccac ggtcactaaa agggcctcaa
      241 gcacctagcc taccgaaaca gccggaggat ttcacatgag cgtcaagaaa gggaaaaaga
      301 aagaacgtcg gattgtccaa agtggagtgg cacatgttca ggcgtcattc aacaatacga
      361 ttgtgaccat cactgacatg agtggaaaca cggtggtgtg ggcaagcgcc ggcaatcaag
      421 gattcaaagg atcgaggaaa agcaccccct tcgctgctca gcgggcgggt gaggctgccg
      481 cgcgaaaagc catggaaagc gggatgcgac aagtcgatgt atatgtgaat ggaccgggct
      541 cgggtcgaga atcggcgatc cgttcacttc aaggcgctgg tttgcggatc aacttgattc
      601 gcgacgtcac gccaattcca cataatgggt gtcggcctcc caaacggcgg cgcgtgtgat
      661 gccgtgcatg gatggtcttc gtcgctgggc gagatgcatg acgtattttc agtgaatcag
      721 taagtcattt tgcgaggagg aagcagtggc aaagtatcgt ggtccagtct gtcggttgtg
      781 tcggcgagaa ggtgagaaac tctttctcaa gggcacgcgc tgtatgacgg aaaaatgcgc
      841 cgtggaacgc aggagttatc cgcctggcca acatgggcag gggcgacagc gaacttctga
      901 ctatagtctg cagttgcgcg aaaaacagaa gctgcgtcga atctatggac ttcaggaatg
      961 ccaattccgc ggcgttttcg aacgtgcaga acggcagacc ggcgtcacag gcgatgcctt
     1021 gttgcgtttg ctcgagtgtc gcttggacaa tgtcgcctat cgcttagggt tcggtgcgtc
     1081 gcgcaagcaa gctcgtcaaa tggtgagtca tggtcacttc actgtaaacg gcaagaagat
     1141 cacggtggcc ggggcactgg tgaagccggg agatgtgatc gaaattcgcg aacggagtcg
     1201 ggacttggcc gcaatccaga cggcattgga atcggtcgat agtcgaggca ttcctgattg
     1261 gcttgagctc gacaaaggag cgttcaaggg caccgttcgc gcattgccgg ccaaggagca
     1321 gatcgcgttg ccggtcaacg aacagatggt agtggaatta tactcgcgat agtccgccga
     1381 tctgaggtgc gttctttcga catcggcgtt atctgccgat gtcttagtca ctcgatggta
     1441 tggagggtgg gtcatgatca aagcgatgaa agactttcag atcccaatgc gggtggaagt
     1501 cgacaaggat gcacattctc ccacatttgg gcgattcacc acggaagcgt ttgaacgggg
     1561 atttggcacc acgatcggta atgccctccg tcgcattttg ctgtcgtcgc tcacgggggc
     1621 tgcggtgacc accgtcaaga ttgaaggggt cgtgcacgag ttttcgacga tctctggtgt
     1681 gacagaagat gtcacggcaa tcattctaaa tgtcaagagt ttgcggcttg cgctccacac
     1741 ggataaaccg aagacgatac ggttgaaa
//
LOCUS       DATMTI010001084         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_591725, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001084 DATMTI010000000
VERSION     DATMTI010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591725"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VN039_17170"
     CDS             <1..891
                     /locus_tag="VN039_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47730.1"
                     /translation="TSDMNPHAMSGNQSTPNLYRLGLQDMLRSGVPFLVGGAFALENY
                     TGVVRRTKDLDVFALRKDVDGLMRFLASAGYETEVRFPHWISKAHKGGHVIDIIFGSG
                     NGICAVDQKWFDAAPVATIMGERVRLVPAEEMIWSKAFVMERDRYDGADIAHLLHARS
                     DRLDWGRLLWRFDRHWRVLFSHLVLFGFVYPRRRLQIPHWLMLELIQRLCMEMQTLSS
                     DRDICQGTLLSWGQYLDHIECGEYKDARRHPCGSLTAGEIKFVTERFQEEQGGYQGTR
                     TVAEDFVLRDGPPRLSEPAA"
     gene            917..1363
                     /locus_tag="VN039_17175"
     CDS             917..1363
                     /locus_tag="VN039_17175"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWV47731.1"
                     /translation="MNDKAYVSLIGRLLIGLIFVMSGIHKIADPQGTQQYMAAMGITT
                     GTTFLYAGAVFMEVAGGLSLWLGVWSKWGATALIIFMMPTTMIFHTNFGDQNQTIHFL
                     KNLAMMGGLLYVAVYGPGRLSIDRGVSRVNQDVESEVPSLKKVVHQ"
     gene            complement(1475..1675)
                     /locus_tag="VN039_17180"
     CDS             complement(1475..1675)
                     /locus_tag="VN039_17180"
                     /inference="COORDINATES: protein motif:HMM:NF025731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HWV47732.1"
                     /translation="MTMPDLGNFSLQTPGTLLVYFIPSFVASARNQENRFAIFALNLV
                     LGWTVIGWIGALVWAIHKPMRV"
BASE COUNT          549 a          600 c          713 g          559 t
ORIGIN      
        1 acaagcgaca tgaaccctca cgcgatgtcg ggcaatcaat ccacgccgaa tctgtatcgg
       61 ctcgggctgc aagatatgct gcgctccgga gttccgtttc tggtaggcgg cgcgtttgca
      121 ttggagaact atacgggcgt ggttcgacgc accaaggatc tcgatgtttt cgcgctccgg
      181 aaagatgtgg acggtctcat gcgattcctc gcctctgcgg gatatgagac agaggtccgg
      241 tttcctcatt ggatcagtaa agcgcataaa ggcggccatg ttatcgacat cattttcggg
      301 tcgggtaacg ggatttgcgc cgtcgatcag aaatggttcg acgcggcgcc agttgcgacg
      361 atcatggggg aacgagtccg gttggtgccc gccgaggaaa tgatttggtc caaagccttc
      421 gtcatggaac gcgaccgtta cgacggggcg gacatcgcgc atctgttgca tgcacgatcc
      481 gaccgtttgg attgggggcg gttgttgtgg agatttgatc gccattggcg cgtgctgttc
      541 agccatctcg ttttgttcgg cttcgtctac ccgcggcgcc gattgcaaat tccccactgg
      601 ctgatgcttg aactcataca acgactgtgt atggaaatgc agaccctgtc gtcggatcgc
      661 gatatctgtc agggaacgct gctgtcctgg ggacaatatc tcgaccacat cgaatgcgga
      721 gagtacaagg atgcccgccg tcatccatgc ggatcgctga cagccggtga gatcaaattt
      781 gtgactgagc ggtttcaaga ggagcaaggc ggctatcaag gaaccaggac ggttgcagaa
      841 gacttcgtgc tgcgtgatgg gcctccgagg ttgtcggagc ccgcggcatg aagacggcgg
      901 aagaaaggtg acgacgatga atgataaggc gtatgtcagt ctcatcggac ggttattgat
      961 cggtttgata tttgtgatgt cgggcatcca caagattgcc gatccccagg ggactcagca
     1021 gtacatggcc gcgatgggca tcacgacagg aacaacgttc ctttacgcgg gagctgtttt
     1081 catggaagtg gccggtggat tatcgttgtg gctgggggtg tggtcaaaat ggggtgcgac
     1141 ggcgctcatc atcttcatga tgccgactac gatgatattc catacgaact tcggcgatca
     1201 gaatcagacc atccactttt tgaagaacct cgcaatgatg ggtggactgc tgtacgtggc
     1261 cgtctacgga cctggacgat tgagcatcga tcggggagtt tcacgggtga atcaggatgt
     1321 cgagtcggag gtaccctctc tcaagaaagt ggtgcaccaa taagggacgc atctcaggga
     1381 cgcctccacg ctcgtcggcg accggatcga accgggaggg gcttttttga atcaccgctt
     1441 ccctcgccgt gccttacgct cgatcatggc tgtatcatac gcgcatcggt ttatggatag
     1501 cccacaccag ggcgccgatc catccgatca ccgtccagcc tagtactaga ttgagtgcga
     1561 aaatcgcaaa acggttctcc tggtttcgtg cggaggcgac aaagctcggg atgaagtaga
     1621 cgagcagggt ccccggtgtt tgcagactaa agtttccaag atccggcatg gtcattacac
     1681 aaaacgatgg tccgtaccgg ggagactaaa acaaatgtcg tgtcacgaaa taactgtaga
     1741 caaggacgct aataattccc gtcacgtacg acacggctgc cgtagcgagt ggatggtgaa
     1801 cgagccattg gacggagttc tgaagaggat tgggagacac catcatgggt caccgccttt
     1861 ctcacaaggt gacggcggtg ggccatccag aacccgtcgg caagatgtgg gttgaatcgt
     1921 agactagtta gggtatctca atggcaaagg atgtccatgc tctcaatccc tagggctgca
     1981 tctttatatt gcaccgtcta acgggtctaa tggagacgag gagcctataa cgattctcca
     2041 tagagtgagg ttcgtagcaa agctagtcgc ttgccccgct atttccacag cgtatctgcg
     2101 atgcgaggag gtaggttttc agttcttctg aataatattg aacgattttt cgctcttcag
     2161 gagataacgg cttgctgcca aagagaaccg cagaggaaag gatcatttcg caggaacgga
     2221 tgaagccatg gatttctttg cgtaacggag agaatggact gctagacgcc gacataggac
     2281 accgcctttc tcacaaggtg cggtcttggc acggttcggt ctgatcccga agggtcgcct
     2341 cgtttttgtc ggcaatcacc tcacgcttag atggcggcac cacctccaat ggttcttcac
     2401 cgacttgacc caccagccat t
//
LOCUS       DATMTI010001085         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_82594, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001085 DATMTI010000000
VERSION     DATMTI010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82594"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VN039_17185"
     CDS             complement(<1..777)
                     /locus_tag="VN039_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWV47733.1"
                     /translation="METVRLEPIRNVAVVSNAGAGKTSLSEALLYAGEAIPMLGSVTH
                     GTTVSDFEPEELRHRNSTSTGLLQFSWNHTNINLIDPPGALDLLGEPLAALQAVDAVI
                     LLLSGNMGVRTELARLWARIKELELPCLVFVNGLDKEGASFENALETCRQQLGRVPIS
                     MTVPIISGTNLDGVIDVRHEKLVRSGPSSAQVEHLPISNDLEKILSGARKQLVEAVAE
                     SGETLLETYLTQGELSTEDLLHGLRRGIQTNQFLPVYAGSA"
     gene            complement(872..>1613)
                     /gene="radC"
                     /locus_tag="VN039_17190"
     CDS             complement(872..>1613)
                     /gene="radC"
                     /locus_tag="VN039_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWV47734.1"
                     /translation="SIFCHLRVFVAGKKTRKGIARWPKTERPRERLLAKGSEALSDAH
                     LLAILLRTGRRDSSAVQVAIELLDHVGGLGGLAACGIEELCAIPGVGPAKAAQLKAAL
                     ALGRRSMAVPMSTGTRISSSADLFKHFHPALRDVKHELFKVVLLDAKHIVIKEATVSE
                     GSLTLSIVHPREVFALAVRESAAAVIFLHNHPSGDPTPSPEDRRLTDRLAMAGKLLGI
                     SVLDHVIIGDGRYVSFADEGWITGQKNE"
BASE COUNT          345 a          456 c          447 g          365 t
ORIGIN      
        1 cgccgatccg gcatatacgg gaagaaattg attcgtctgg atgccgcgtc ggagtccatg
       61 aagaaggtct tccgtgctta actcgccctg agtcaagtag gtctccaata aagtttcccc
      121 actctccgcg actgcctcca cgagctgctt gcgcgctccg ctgagaattt tttccaggtc
      181 gtttgaaatc ggtaggtgtt cgacttgcgc ggaactcggt ccagaccgta caagcttttc
      241 atgtcgtaca tcaattacgc cgtctaagtt cgtgccagag atgatcggca ccgtcataga
      301 gattggaaca cgaccgagct gctggcggca ggtctccaga gcgttctcga acgatgcacc
      361 ttccttatca agcccgttca cgaacaccag acagggaagt tccaactctt tgattcttgc
      421 ccacagccgt gccagttccg tccttacccc catgtttccg ctcagaagaa ggatgaccgc
      481 atcgacggcc tgcagggccg cgagcggttc gcccagaaga tcgagggcgc cgggagggtc
      541 aatgagattg atgttggtgt gattccatga gaactgaagg aggccggtac ttgtcgaatt
      601 gcgatgacga agctcttccg gttcgaaatc agaaacagtg gtaccgtgcg tcacggaacc
      661 aagcatgggg atcgcttcac cggcatacag caaggcctca ctgagagagg tcttgcccgc
      721 accggcgttg gatacgaccg ccacattcct gatgggctcc agacggactg tttccatagt
      781 ctgccctcct tgttgagtag gggtttaggc cattcgctgc ctcatattcc tcggtgcggt
      841 ggtttcgatc gacatgattt cagaggcccg ctcactcatt tttctgtccc gttatccatc
      901 cttcgtcggc aaagctcacg taccgaccgt ctccaataat cacatggtcc aaaacgctga
      961 tccccaacag cttgccggcc attgcgagtc gatcggtcaa tcgccgatcc tccgggcttg
     1021 gagtcggatc tccgctggga tgattgtgaa gaaaaatgac ggcggcggcc gattcacgta
     1081 ccgcgagcgc aaagacctcg cgtggatgca cgatgctgag cgtgaggctt ccttctgaaa
     1141 ccgtggcctc tttgatcacg atatgttttg cgtccagcag cacgaccttg aagagttcgt
     1201 gcttcacatc gcggagtgca gggtgaaaat gtttgaatag atccgcactt gaggaaatac
     1261 gagtgccggt ggacattgga acggccatcg accgtcgccc taatgccaac gcagccttga
     1321 gttgggctgc tttggcgggg ccaacgcccg ggatggcaca gagttcttca atgccgcagg
     1381 ccgccaatcc tcctagccca cctacgtggt cgagaagttc aatggccacc tgcaccgcgg
     1441 acgaatcgcg gcgaccggtt ctcaggagga tggcgagaag atgggcatcg gataaggcct
     1501 cagatccctt ggcaagaagg cgctcacggg gacgttcggt ctttggccag cgcgcaatgc
     1561 cttttcttgt ttttttgccc gccacgaaga ccctcagatg acaaaaaatt gac
//
LOCUS       DATMTI010001086         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_168271, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001086 DATMTI010000000
VERSION     DATMTI010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168271"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VN039_17195"
     CDS             complement(<1..187)
                     /locus_tag="VN039_17195"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWV47735.1"
                     /translation="MSRYPSMSRLTVRKAVIPAAGLGTRFLPATKASPKEMLPLVDKP
                     LIQYTVEEAVASGIEDII"
     gene            662..>1522
                     /locus_tag="VN039_17200"
     CDS             662..>1522
                     /locus_tag="VN039_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HWV47736.1"
                     /translation="MTEHAVYMPRSFVVAVMVASVLWSVLEALAQTPDLKIASIDIRG
                     AKRIEVPAIAGRLTLKVGDRYTPDNVRGQVKILYDTGFFEDVQVETESIAEGMAVTFV
                     VQEKPFITEIVFDGNEQLTDEKLKEKTTIKSQTFLDQQQVKESAETIRLAYQHDGYYN
                     AQVVPVIEALDEERKRLTFHITEGEKAKVKTVTFEGLRAATKTEMFNVMSTREWIPWY
                     GLVTRLKLPSVVSDAGVLKREELTNDVERIKEVLLNKGYFNVRVGQPSVELTEDKKWF
                     IVSYHITEGEP"
BASE COUNT          373 a          362 c          466 g          321 t
ORIGIN      
        1 cgataatatc ctcgatgccc gacgccactg cttcttcgac ggtgtattga atgagcggtt
       61 tgtcgaccaa cggcaacatc tctttagggg atgcttttgt ggctggaagg aatcgtgtcc
      121 caaggccggc cgcgggaatg accgcttttc tgacagtcag acgtgacatg gaaggatacc
      181 gtgacatgga aggatactat gtgatggggt gtgtgaagtc aagaaacgga cgaactcatc
      241 gcatcgaaac gatggcatcg tttccgtgta aagaagacgc gagcgattct cacgaatagg
      301 cgtgtgccgt gccattttgc agaatgcggg ggcccgtcgc gatcgatgcg atggggcaca
      361 ggatcagaac caagcaagat gcacgcacgt cgaaccagaa ggtgggacac ttctttacaa
      421 tgcgggatga cctcaatata atccggaagt tttgcgcggt cggtggtacg aaggacgttc
      481 gcgtggtaga gctgccgggc tgcaaattcc cgacgaaagg gctcttacgg ttcagcgcgt
      541 gctccggagc gggactgatt gactcgtcgg gagttggcgt acggtcgata acggagagga
      601 cctgtgtgat gattccaacc gcatcatcca tctccatact tcgccacagg aatccttgtc
      661 ggtgaccgag catgccgtct acatgcctcg atcgttcgtg gtcgcggtca tggttgcctc
      721 tgtgttgtgg agcgtgctcg aagcactcgc tcagacgccg gacctcaaga tcgcttcgat
      781 tgacattcgg ggagccaaac gaatcgaagt tccggcgatt gccggtcgac tcacgctgaa
      841 agtcggcgat cgctatacgc cggacaatgt gcgcggacaa gtgaagattt tatacgacac
      901 gggttttttc gaggacgtac aggttgaaac cgaatcgatc gccgagggga tggcggtgac
      961 cttcgtcgtg caagagaaac cgttcatcac tgagatcgtc ttcgacggga acgagcaatt
     1021 gaccgacgag aagctgaagg aaaaaacgac gatcaagagc cagacatttc ttgatcaaca
     1081 acaggtaaag gaaagcgccg agaccattcg cctggcctac cagcatgacg gatattacaa
     1141 cgcgcaggtt gttcccgtga tcgaggcgtt ggatgaggaa cggaagcgcc tgaccttcca
     1201 tattacggag ggagagaagg ccaaggtcaa gacagtgacc ttcgaagggc tgcgtgcggc
     1261 aacgaagacc gagatgttca atgtcatgtc gacgcgggaa tggattcctt ggtacggtct
     1321 tgtgacgagg ctcaaattgc cttcggtcgt atccgacgcg ggtgtcttga agcgtgaaga
     1381 gctgacaaat gatgtggagc ggatcaagga agtgctgctg aacaaaggct atttcaatgt
     1441 tcgggtcggc cagccgtcgg tggagctcac cgaagataaa aaatggttca tcgtcagtta
     1501 ccacatcacc gagggagagc cg
//
LOCUS       DATMTI010001087         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_508093, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001087 DATMTI010000000
VERSION     DATMTI010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508093"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1406)
                     /gene="ahcY"
                     /locus_tag="VN039_17205"
     CDS             complement(147..1406)
                     /gene="ahcY"
                     /locus_tag="VN039_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWV47737.1"
                     /translation="MDYDVKDIKLADQGRSKIEWAEATMPVLRLIRKRFKRQQPLKGV
                     RVTACLHVTTETANLVITLKAGGADVRLCASNPLSTQDDVAAALVQHEGIPTFAIKGE
                     DNATYYRHIESAIAHRPHVTMDDGADVVSHLHSKRKELLRNVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSVVVVVGYGWC
                     GRGIAMRAKGMGADVIVTEIDPLKGLEAVMDGFRVMPMEQAAPVGDFFVTVTGNIHVI
                     RGEHFVAMKDGAIVCNSGHFNVELDIPALEKLAGTRRVVRTGVEQFTLKKNGHRVSLL
                     GEGRLVNLATAEGHPSSVMDMSFANQALSTEHIVKNYKQLEKKVYPVPPAIDKEIARL
                     KLAGMGVSIDTLTAEQKKYLASWEMGT"
     gene            complement(1525..>2653)
                     /gene="metK"
                     /locus_tag="VN039_17210"
     CDS             complement(1525..>2653)
                     /gene="metK"
                     /locus_tag="VN039_17210"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWV47738.1"
                     /translation="TSESVTEGHPDKIADQISDGILDAIIAQDKYSRVACETILTTGI
                     AFVAGEISTKAYVEIPDIIRDVIKDVGYCDASWGFDFHTCSVLTAIHQQSGDIAMGVD
                     SGGAGDQGLMFGYATNETDELMPMPLVLAHRLTKRLAEVRKKNILKWVRPDGKSQVTV
                     EYKNGRPVRIDTIVVSTQHSPDVTTKQLERDIMEKVIKPVMPKGLYDPTGVKHHINPT
                     GRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPTKVDRSASYMARYIAKNL
                     VAAGLADKCEVQLAYAIGVADPVSVLVDTKGTEKVSVDILDKLVRKHFPMTPRGIIDH
                     LKLRRPIFRKTAAYGHFGRTEPEFTWEKIDKTKALRKDAGL"
BASE COUNT          544 a          747 c          750 g          612 t
ORIGIN      
        1 ctcagcgagt atcgcccgaa tcaggatgct caaaatggca tcccggctag gccgcaggga
       61 gctcggcgac cgaagcgtac ccttgcggta cgttgcaggg agacgagcga ccgagaacaa
      121 cgcgggggga cattttcagc atcctgctac gtgcccatct cccaggatgc aagatacttc
      181 ttctgctccg ccgtcaatgt gtcgatcgac acgcccatgc cggccagctt gagccgggcg
      241 atttctttgt cgatggccgg aggcacggga tagactttct tttcgagctg tttgtaattc
      301 ttgacgatat gctccgtgct gagtgcttgg ttagcaaagc tcatatccat gacgctggac
      361 ggatgcccct ccgctgtggc gaggttgacg agccggcctt cgccgagcaa actgacacga
      421 tgaccgttct tcttgagcgt aaattgctcg actcccgtac ggaccactct gcgcgtgccg
      481 gcaagttttt cgagggccgg gatatccaac tccacgttga agtgcccgct gttgcagacg
      541 atggcgccgt ccttcatggc gacaaaatgt tcgccacgga tcacatggat gttgccggtg
      601 acggttacga agaaatctcc gaccggagcg gcttgttcca tcggcatcac gcggaaacca
      661 tccatgacgg cttcaagacc tttcaacggg tctatctcgg tcacaatcac gtcggccccc
      721 atgcccttgg cccgcatggc gataccgcgc ccgcaccagc cgtagccgac aacaacaacg
      781 acggagccgc agaccaagcg gttcgtggcg cgcacaatgc cgtccatggt ggattgaccg
      841 gtgccatagc gattgtcgaa catatgcttg gtatcggcgt cgttgactga aatgaccgga
      901 aacttgagga ccttcttttc cgccatgctg cgcaaccgga tcacgccggt ggtcgtttct
      961 tccgtacccc caatgacgtt ccgcaacaat tcttttcgct tagaatgcag gtgcgagacc
     1021 acgtcggcac cgtcatccat ggtgacatgg ggccggtgcg cgatggcgga ctcgatgtgg
     1081 cgatagtacg tggcgttgtc ttcgcccttg atcgcaaagg ttgggatgcc ttcatgttgt
     1141 actaaggccg cggcgacatc gtcctgggtg ctgagggggt ttgaggcaca gaggcggaca
     1201 tcggcgccgc cggctttcag tgtgataacc aggtttgccg tttcagtggt cacgtgcaaa
     1261 caggctgtga cgcgaacgcc cttcagcggc tgttgtcgtt taaaacgctt acgaatcaac
     1321 cgcaacacag gcatcgttgc ttcggcccat tcgatcttcg atctgccttg gtccgccagc
     1381 ttgatatcct tgacatcgta atccacgaaa gtcctcctcg tcgaaaagaa ggctcaggaa
     1441 aggtgaggct gagtcagaaa cccaacctct cctaagcctg agcctcagtt attaaatctt
     1501 aaccttggcc tcagcctgtg gtcgttacag tccggcgtcc ttgcgcaatg ccttggtctt
     1561 atcgattttc tcccatgtga attcaggctc ggtgcgaccg aagtgtccgt acgcagctgt
     1621 cttgcggaaa atcggccgcc gaagcttgag gtgatcgatg atgccacggg gagtcatcgg
     1681 gaaatgctta cgcacgagtt tgtcgagaat atcgaccgat actttttcag tgcccttggt
     1741 gtcgacgagt acggacaccg gatcggcgac cccgatcgca taggccagct gcacctcgca
     1801 cttgtcggcc aagccggcgg ccacgagatt cttggcaata tagcgggcca tataggaggc
     1861 ggagcggtcc acctttgtgg gatcttttcc agaaaaggca ccgccgccgt ggctgccgtg
     1921 accaccatag gtgtcgacga taatcttccg gcctgtaagg cctgtgtcgc ccattgggcc
     1981 accgaccacg aaacgaccgg tcgggttgat gtgatgtttg acgccggttg gatcatagag
     2041 acctttgggc atcacgggct tgatgacttt ttccatgatg tcgcgctcaa gctgcttggt
     2101 ggtcacatcg gggctgtgtt gtgtggagac gacgatcgta tcgatccgca cgggcctgcc
     2161 atttttatac tcgaccgtca cttgggattt gccgtctggc cgcacccact tcagaatgtt
     2221 cttcttgcgc acctctgcta ggcgcttggt cagacgatgg gccaagacga gcggcatcgg
     2281 catgagttca tccgtttcat tggtggcata gccgaacatc aagccctgat caccggcccc
     2341 tccagaatcc actcccatcg cgatatcacc ggattgctgg tgaatggcgg tgagaaccga
     2401 gcaggtatga aagtcgaatc cccatgaagc atcgcaatag ccaacgtcct tgatgacgtc
     2461 acgaataatg tctgggattt caacataggc cttggtggaa atttctcccg ctacaaaagc
     2521 gatgccggta gtcaggatcg tttcgcaggc gacgcgggaa tatttatcct gagcaatgat
     2581 ggcgtctaag atgccgtcgg aaatctggtc ggcgatctta tctggatgtc cttcagtgac
     2641 cgactccgaa gta
//
LOCUS       DATMTI010001088         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_591953, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001088 DATMTI010000000
VERSION     DATMTI010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591953"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VN039_17215"
     CDS             complement(<1..229)
                     /locus_tag="VN039_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47739.1"
                     /translation="MINTEISKHNNRRSVALALVLGIEMLISQIPGTTRADQQPPMMT
                     VGYQSGTITSIYETTFEIDHRTYSLTPDAALL"
     gene            complement(284..823)
                     /locus_tag="VN039_17220"
     CDS             complement(284..823)
                     /locus_tag="VN039_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47740.1"
                     /translation="MTVMIITLMAGALGVAALTMTGLENSTAGAMRMVEEATDAAESC
                     VGMGVKVIQQAIETGAVPPTLVAPNGPVPASNALFLGQEINYLANNSDVAWGSGVVAP
                     NITMNVNNYVVNGDIDKQFVKIRAGSQNTGGSSQAPPTDSYYRIDCQAVNAATGVTSR
                     VIAVYQCLVSGDTCQSRFL"
     gene            complement(892..1911)
                     /locus_tag="VN039_17225"
     CDS             complement(892..1911)
                     /locus_tag="VN039_17225"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HWV47741.1"
                     /translation="MITADLKLAGFGMQGLMGGPVGNCVVNGMPAAVIPADNNALGAD
                     FGPDSISMVVPMTNSIAAVGPMWQLFIAASGTIGGPNQPIATLPLINNATTGMGTAIP
                     GGAAALPGMSVTVGGVAGSRIAAGGVTPAALTVNPAIPAPATFGTGTQVYLLQCITYQ
                     VIPPPDALNLCQGNAPCLVRGVAPVGVGGPPNCNNPGNTCVPIMDGVEDLQLAYACDG
                     CNGSGVPDGAIDDMNGSNSFDQGDFLTNINWFLPPNGMMTPNKIRMVQVSIIARQTRT
                     DQGMGEANQIVVHSTAIPTISDHNHATGVFALGDNNTAAQQQAYFQFRRRILSRTVEL
                     RNQRS"
     gene            complement(2141..2653)
                     /gene="pilV"
                     /locus_tag="VN039_17230"
     CDS             complement(2141..2653)
                     /gene="pilV"
                     /locus_tag="VN039_17230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HWV47742.1"
                     /translation="MKCGNEKGFTLVEVLVSAAILGVGVMGMAAMQGMALSKNVDAND
                     LSMVTNIASDMMERVQANRQYAWAYHNLQTLGPGNCAALPAPPPTGITKTTPVNIVRV
                     VQGDCTQWRTLVLASNLTNVQGLIQVTNGVPANPLLGARQVVVQVQWNDRGAGQRLRT
                     VSFQTTLVPE"
     gene            complement(2673..>2879)
                     /locus_tag="VN039_17235"
     CDS             complement(2673..>2879)
                     /locus_tag="VN039_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47743.1"
                     /translation="LPLRVTFFLVPAQPIGFTPRGLSTTPLATQTIQLQSVRDPNLIY
                     SISLAPSGKVTWCTRPINPCIQNQ"
BASE COUNT          634 a          782 c          729 g          734 t
ORIGIN      
        1 ctaagagggc tgcgtccggc gtcaaactat aggttctatg gtcgatctca aacgtcgttt
       61 cataaataga tgtgatggtt ccgctctggt atccgaccgt catcatcgga ggctgttgat
      121 ccgctcgagt cgtcccggga atctggctga tcaacatttc aattcccaag actagcgcca
      181 gtgccacaga gcgccgattg ttgtgtttac ttatctctgt attgatcatg gaccgatcct
      241 ccattcactt gttttagcta caagtgagcg cctctgttat ctgttagagg aagcgcgact
      301 ggcacgtatc accagacacg aggcattgat agacggcgat gacacgactg gtcacccccg
      361 ttgccgcatt caccgcctgg cagtcgatgc ggtaatagct atccgttggt ggcgcctgtg
      421 agcttcctcc ggtattttgc gaacctgcac ggatcttgac gaactgcttg tcgatatcac
      481 cgtttaccac gtaattgttg acattcatcg tgatgttcgg cgcgacgaca ccgcttcccc
      541 acgctacatc agaattatta gctaggtaat ttatctcttg cccgaggaat agtgcgttgc
      601 tggccgggac cggtccattc ggcgcaacca aggtcggcgg gacagctcct gtctctatcg
      661 cctgttggat gaccttgact cccatcccaa cacaggattc agctgcatcg gtagcttctt
      721 ccaccatacg catagcaccg gccgtagagt tttccagacc ggtcatggtc agagctgcaa
      781 cgccgagcgc accggccatg agggtaataa tcatgaccgt catgaatgac acgcctcgtt
      841 catcgaggac tttttccttt agtctctcag acttgctaca taactccatt gttacgacct
      901 ctggtttctg agttcgactg ttcgcgacag aatccgtctc ctaaactgga aatacgcttg
      961 ttgttgcgct gccgtattgt tatcaccgag ggcaaacaca ccggtcgcgt gattatgatc
     1021 gctgattgta ggtatcgccg tgctatgcac gacgatttgg ttggcctctc ccattccttg
     1081 atcggttctg gtttgccttg ctatgatgct gacctggacc atgcgaatct tgttcggtgt
     1141 catcatgccg ttcggaggga gaaaccaatt gatgttggtc agaaagtctc cctggtcgaa
     1201 tgaattcgac ccattcatgt catcaatagc accatctggt actccggatc cgttgcagcc
     1261 atcacaggca taggccaatt gtaagtcttc cactccatcc atgatcggta cacaggtgtt
     1321 acctggattg ttgcaatttg ggggtccacc gacgccgacc ggagccacgc cgcgaaccaa
     1381 acaaggcgcg ttcccttgac agagattgag ggcgtccgga ggaggaatga cttgataagt
     1441 gatgcactgc agcaggtaca cctgtgtacc ggttccaaat gtggcgggtg caggaatcgc
     1501 tggattcaca gtgagagcgg caggggttac acctccagcc gcaatgcgtg atcctgcgac
     1561 acctccaact gttaccgaca tcccaggaag cgccgctgct ccgccgggga ttgctgtccc
     1621 catacctgtt gtcgcattgt tgatcaaagg gagagtagcg atcggttgat tagggccgcc
     1681 aatcgttcca gaagctgcaa taaagagttg ccacatcggt cctaccgcgg caattgaatt
     1741 cgtcatcggc acgaccatcg atatcgaatc cggtccaaaa tctgctccga gagcattgtt
     1801 atccgctgga atcaccgccg caggcatacc attcacgaca cagttaccaa ccggcccacc
     1861 catcagaccc tgcatgccga agcctgcgag cttgaggtca gcagtgatca tgtcaagtcc
     1921 gctccttgcg gttgcctggg tattcaccac ttgatttgtc actctagccg tcttttgagt
     1981 gaccgtcaga gctcctagtc cagccgcaat gattgctgtg gtcatcatcg tcgcgaccat
     2041 tacttccatc agggtaaatc ctcgtgagct aactaaggtt ggcgttggag atatgaccat
     2101 cgatacaccc atttgttgct cagttaagcc ataattaatt tcattcgggg accagcgtcg
     2161 tctgaaaact gacggtcctg agtcgttgcc cagcacccct atcattccat tggacctgca
     2221 caaccacttg ccgagccccc agtaaaggat ttgccggaac gccgtttgtt acctggatta
     2281 gtccttgtac attggtcaga ttcgatgcca gcaccagggt acgccactgt gtgcaatcac
     2341 cctgaacgac tcgaacgatg ttgacaggag tggttttggt aatgccagtc ggcggtggag
     2401 ctgggagtgc tgcacaattt cccggtccaa gtgtctgaag gttgtgatag gcccaggcat
     2461 actgccgatt ggcttgaact cgttccatca tgtctgaagc gatattggtg accatggaaa
     2521 gatcattggc atctacattt ttggatagcg ccatgccttg catcgcagcc attcccatca
     2581 caccgacacc aagaattgcc gctgatacca gaacctctac taaagtgaag cctttttcat
     2641 tcccgcattt catctcgtca cctgctttct ctctactggt tctgtatgca ggggttgatc
     2701 ggcctggtac accaggtgac cttgccggat ggagccaacg aaatcgaata gattaaattg
     2761 gggtcacgga cgctctgcag ctgaatcgtc tgagttgcca atggggtagt actcaggcct
     2821 ctcggtgtaa atccaatcgg ctgtgctggc acgagaaaaa aggtaacgcg tagtgggag
//
LOCUS       DATMTI010001089         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_805527, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001089 DATMTI010000000
VERSION     DATMTI010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805527"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /gene="panB"
                     /locus_tag="VN039_17240"
     CDS             complement(<1..492)
                     /gene="panB"
                     /locus_tag="VN039_17240"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWV47744.1"
                     /translation="MTILDFQQFKRDRKKLAVVTAYDALFACIVEQAGIRVILVGDSL
                     GMVVQGKPNTLAVTMDDMLYHTRLVAGVVQRALVIADMPFMSYQTSTEEALRNAGRLL
                     QAGAAAVKLEGGAVMVDRIKAMTSVGIPVMGHLGMTPQSVYALGGYKVQGKADDQASR
                     LLQD"
     gene            complement(492..1379)
                     /locus_tag="VN039_17245"
     CDS             complement(492..1379)
                     /locus_tag="VN039_17245"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWV47745.1"
                     /translation="MSREPQRLIDVLNRGTFAVTVEYNPPKGTNISSVLESAKQLVGR
                     VHGVNVTDNTAAVVRAGSLPVCRLLYELGHDPVMQLTCRDRNRIAMQSDLMGGHMLGI
                     RNILCLTGDYPTVGDHKEAKPVYDLDSVQVMQLVQGLNKGHDLAGHKLDGSTAFTIGA
                     AVTPEADPLGPMLAKFEVKVKAGAQFFQTQAVYHPEQFASFMKAVQPYKVKVLAGILV
                     LRNHKMAEFMNGNIPGISVPSEMIDELKAAGEKAEDVGVDIAVRTIKAVRPHCDGVHI
                     MAIKATHRLAEIITKAELN"
     gene            complement(1391..>1857)
                     /locus_tag="VN039_17250"
     CDS             complement(1391..>1857)
                     /locus_tag="VN039_17250"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HWV47746.1"
                     /translation="EGNPVFEACTPKGVIKMIESTGMTIEGKRAVVVGRSNIVGKPLA
                     LMLLQRNATVTICHSKTKDLPGVCREAELLLVAIGKAKFVTADMVREGAVVIDVGTNK
                     TPEGKLCGDVDFDQVSQKAGWISPVPGGVGPMTIAMLLENTVESAKRAAGML"
BASE COUNT          383 a          586 c          467 g          421 t
ORIGIN      
        1 atcctggagc aacctagaag cttgatcgtc ggctttacct tggaccttgt atccgcccaa
       61 cgcatagact gattgtggtg tcatacctag gtggcccatg acaggaatgc cgacactcgt
      121 catggctttg attcgatcta ccatcacggc ccctccttcc agcttcaccg cagcggcacc
      181 tgcttgcagc agtcgacccg cattgcgcag ggcctcctct gtgctggtct gataggacat
      241 aaacggcata tccgcaatga ccagagcccg ctgcaccacg ccggcgacca gcctggtgtg
      301 atacagcatg tcgtccatcg tgaccgcgag cgtattgggt tttccctgta caaccatgcc
      361 cagcgaatcc ccgaccaaaa tcacccggat tcccgcttgc tccacaatac atgcgaacag
      421 cgcgtcatag gccgtcacga cggcgagttt cttcctgtct cgtttgaact gctggaaatc
      481 gaggatcgtc atcagttcaa ttcagctttg gtgatgatct cggccagccg atgcgtggct
      541 ttgatcgcca taatatgaac gccgtcgcag tgcggccgaa ccgccttgat cgtccgcacg
      601 gcgatgtcga ctccgacgtc ctctgccttc tcaccggcgg ctttgagctc atcgatcatc
      661 tcgcttggaa ccgagatgcc cggaatattc ccgttcatga actccgccat cttgtggttg
      721 cgcaacacaa gaataccggc aagaaccttg accttataag gctggaccgc tttcatgaaa
      781 cttgcgaact gctccggatg gtacaccgct tgcgtctgaa aaaattgggc acccgctttc
      841 acctttactt caaacttggc gagcataggt ccgagtgggt cggcctccgg tgtgacggcg
      901 gcaccgatcg taaacgccgt cgagccgtcc agcttgtgcc cggccaggtc atgccccttg
      961 ttcaatcctt gcaccagttg catgacttgg actgaatcga gatcatacac cggcttggcc
     1021 tccttatgat caccgaccgt cggataatcg cctgtgaggc acagaatatt tcgaatccca
     1081 agcatatgcc cgcccatcag atccgactgc atggcgatcc gattgcgatc acgacaggtc
     1141 aactgcatca cagggtcgtg ccccaattca tacaggagcc ggcagaccgg gagtgagccg
     1201 gcacgaacca cagcggcggt attgtccgtg acgttcacgc cgtgtacgcg cccgacaagc
     1261 tgtttggcac tctcgagaac ggacgagatg ttggtgccct tgggagggtt atactcgacc
     1321 gtgacggcaa acgtccctcg gttgaggaca tcgattaggc gctgtggctc tcgactcata
     1381 gatggccccc tcatagcatt cctgctgccc tcttggctga ctccaccgta ttttccagca
     1441 gcatcgcaat ggtcatcggg ccgaccccac cggggacagg gctgatccaa ccggccttct
     1501 gactgacctg gtcaaaatct acatcgccgc acaacttgcc ttcaggagtc ttgttagtcc
     1561 ctacgtcgat gacgaccgca ccttcgcgca ccatgtcggc agtgacaaat ttcgcctttc
     1621 cgatcgcaac gaggagcaac tctgcttcac ggcaaacccc aggcaggtcc ttcgtttttg
     1681 aatgacagat cgtgaccgtt gcgttccgtt gaagcagcat caacgccaac ggcttgccga
     1741 caatattgct ccgtcccacc acaactgccc gcttgccttc tattgtcatc ccggtcgact
     1801 cgatcatctt gataaccccc ttgggagtgc aggcttcgaa gacggggttc ccctcca
//
LOCUS       DATMTI010001090         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_677456, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001090 DATMTI010000000
VERSION     DATMTI010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677456"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /gene="aceE"
                     /locus_tag="VN039_17255"
     CDS             complement(<1..1681)
                     /gene="aceE"
                     /locus_tag="VN039_17255"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWV47747.1"
                     /translation="MSDDHPTPQLQADTDPQETIEWLDSLEYVLQVGGVERATYLLER
                     LRDRLGERGARISRPFNTPYINTIPADRQPAYPGNLELERRIRSLVRWNALAMVVKAN
                     KEHSGIGGHISTFASAATLYEVAFNHFLRGKDSPDGGDLLYIQGHASPGIYARGYVEG
                     RFSEDALHHFRREITGEGKGLSSYPHPWLMPDYWEFPTVSMGLGPIMSIYQARFNRYL
                     RDRGLKDTSHQRVWAFVGDGEMDEPESFAALTLAAREQLDNLTWVVNCNLQRLDGPVR
                     GNGKIIQELEAIFHGAGWNVIKVIWGTDWDPLLAQDDEGLLVRRMGEIVDGWYQKYTV
                     EGGAFARKHFFGADPRLLKMVASYSDEQIQKLLRGGHDPRKVYAAYKAAMDHRGQPTV
                     ILAKTIKGYGLGEAGEGRNVTHQQKKMNEQELREFRTRFSVPISDEQVAATPFFRPAV
                     GSPEATYLAERIKVLGGSLPKRQVKIDPLQTPSLDQFKEFLEGSNDRAFSTTMGFARM
                     LGRLLGQKPFGKHLVPIIPDEARTFGLEALFRQYGIYSHLGQLYEPADKSSL"
BASE COUNT          347 a          526 c          513 g          372 t
ORIGIN      
        1 gcagggagct tttgtccgcc ggctcataca gctggccgag atgtgaatag atgccgtatt
       61 ggcggaagag cgcctccaga ccgaacgtac gcgcctcgtc cggaatgatg ggcacgagat
      121 gtttcccaaa tggtttctgt cccaacagac ggcccagcat gcgggcaaag cccatcgtcg
      181 tggagaacgc ccgatcgttc gatccttcga ggaactcctt gaactggtcg aggctcggtg
      241 tctgcaacgg gtcgatcttc acctgccgct ttggaagaga gccacccagg acttttatcc
      301 gctcggcgag gtaggtcgct tccgggctgc cgaccgcagg ccgaaaaaac ggcgtcgcag
      361 cgacttgttc gtccgatatg ggcacgctga atcgtgtgcg gaactcgcgc aactcctgct
      421 cattcatctt tttttgctga tgagtgacgt tgcgcccttc ccccgcctct cccaaaccat
      481 accctttgat cgtcttggcg agaattacgg tcggttggcc gcgatggtcc atggcggcct
      541 tgtaggcggc gtacactttc ctcggatcgt ggccgccgcg cagcagtttt tggatttgct
      601 cgtccgaata ggaggccacc atcttgagta gacggggatc agcgccgaag aaatgtttcc
      661 gggcaaaggc accgccttcc accgtatatt tttgatacca gccgtcgacg atttcgccca
      721 tccgtctcac cagcaggcct tcatcgtcct gcgccagcaa cggatcccaa tcggtgcccc
      781 agatcacctt gatgacattc cagccggcgc cgtggaatat ggcttcgagc tcctggatga
      841 tcttgccgtt gccacggacg ggcccgtcga gacgttgaag attgcagttg acgacccatg
      901 tgagattatc cagctgctcg cgggcggcga gcgtcagcgc ggcgaagctt tccggctcat
      961 ccatttcccc gtcgccgacg aaggcccata cgcgctggtg gctcgtatcc ttcaggccgc
     1021 gatcccggag gtaccggttg aaacgcgcct ggtagatcga catgatcggg ccgaggccca
     1081 tcgagacggt cggaaattcc cagtagtccg gcatcagcca aggatgggga taggaggaca
     1141 agccctttcc ctcgcccgtg atctcgcgtc ggaagtgatg gagcgcgtct tccgaaaaac
     1201 ggccctcgac atagccgcgc gcgtaaatcc ccgggctggc atgcccttgg atataaagca
     1261 agtcgccgcc atccggtgag tctttgccgc gcaaaaaatg gttgaaggca acctcgtaca
     1321 aggtggccgc cgaagcaaat gtcgaaatat gtccgccaat accggaatgc tctttatttg
     1381 ctttgacgac catcgccaga gcgttccacc gaaccagact tcggatccga cgctcgagct
     1441 cgaggttgcc gggataggcc ggctgccggt cggcaggaat cgtattgatg tacggtgtgt
     1501 tgaagggcct ggaaatgcgc gcccctcgct cacctaaccg gtcccggaga cgctccaaga
     1561 gataggtcgc ccgctcgacc cctcctacct ggaggacata ctcaagggag tccagccact
     1621 cgatggtttc ctgcggatca gtatcggcct gaagttgcgg agtggggtgg tcgtcgctca
     1681 ctgtccaagt attcctgaga atcgggggcc cgttaacacg cgctgatcat agcaagcgga
     1741 tctttctaat gcgaatgc
//
LOCUS       DATMTI010001091         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_168393, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001091 DATMTI010000000
VERSION     DATMTI010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168393"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..>2485
                     /locus_tag="VN039_17260"
     CDS             25..>2485
                     /locus_tag="VN039_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47748.1"
                     /translation="MAVAAVSFSDATAAQDLANLANSMIANWTFTGTTNIDGGQIRTD
                     TVTAAQIIAGAIGTSELAANAVTAAKIAAGTITSNEIAANTITAADIAANTITAASGV
                     IANAAITNAMIANITADKIQTGTLGAQTITIGSGGAVQSSNYSSGTAGYKISSTGVEM
                     NDSGSTIKADAIKAGTLGGSGGSGVIQVAAGTSLILNGGYLKSNTYTGTSQATNPSGA
                     GFYLGNDGLRVDSGIISASALTAGTISGTNTITLSGANAKIVGGTWSLSGSGLTIPNG
                     GIAAAALNIQTGSNLMPPAYADFEFQPSYYTGKLATTSATATVVSTQQRFNSQSLSYS
                     PTNATNSVYLGASSTDYNIIVSPSTSYLVSYYAMVATGDTVATVTPTLTYATATTPGT
                     LAGTGTSTPANSTWARYTSVVAVPATATGPSILSFKTNTATGTVFIDGIQIEQKLSNS
                     STASTWTPPGATFIAGDMIKTGSIQSTATTVVNSVTIPVWSIPLNGSASFANVQVRGN
                     AVVGVNNDDDGGFSQIASGNYSAGDAGWVIRSDGYAEFRNMASDSIDGGAIRTKTLSV
                     DTLTSGTLSDEIGLGSGTSLIATGLMGETVGLGDYGFLVTGPQQNVVTFRASSANTRT
                     LTTSVPHNFVATDVDGTPGSKIRVSIGSTDYDTPPGSAYYVDDVPTTTTFTYTASTSL
                     TESTTAVTNGVVQGSNPDLISSSPVYISFPTDGTQPNIISGQLTADQVTVTQGMSIRL
                     SSQIEQGAVLTINSVIQPPSAGPAMSQSYSGVSFQLPTSGYITGIARSTNGNHLCPVG
                     GYSGKECWVNEYDANGKKVAER"
BASE COUNT          639 a          673 c          618 g          555 t
ORIGIN      
        1 ggagcgtccg accccaactc gcacatggct gttgcggcgg tgtcattctc agacgccacc
       61 gccgctcaag acctagccaa tctagcaaac tccatgattg cgaactggac tttcaccgga
      121 acgacaaaca ttgacggtgg acagattcga accgacacgg ttacggcagc acagattatt
      181 gctggtgcca tcggcactag cgaattggct gccaatgctg tcactgctgc caaaattgca
      241 gccggaacca tcacttccaa tgaaatcgct gctaatacaa taaccgcagc cgacatagca
      301 gccaatacca ttaccgccgc gagtggtgtc atcgcaaatg ctgcaatcac aaacgcgatg
      361 attgcaaata tcactgccga caagattcag acaggaacgc ttggcgctca gaccatcacc
      421 attggtagcg gtggggcggt ccagtcgagc aactatagtt ctggaacggc aggctacaag
      481 atttcctcta ccggcgtcga gatgaacgac tctggctcta ccatcaaggc agatgccatc
      541 aaggcaggca cgctaggtgg gtctggaggc tctggcgtaa ttcaggtggc cgcaggtacc
      601 agtctaattc tgaacggtgg ctatctcaag tcgaacacat acaccggcac gagccaggcc
      661 acgaacccgt caggcgcagg cttctacctc ggtaatgatg gtctgcgagt agactctgga
      721 atcatctctg ccagtgcgct cacggcgggt acgattagtg gtacgaacac catcacattg
      781 tctggtgcca atgcaaagat tgtcggaggc acatggagcc tgtctggctc tggactgaca
      841 attcctaatg gcggtattgc tgctgcggcc ctgaacattc agaccggctc gaacctcatg
      901 ccgccagcgt atgcagattt cgagttccag ccatcatatt acacgggcaa attggccacg
      961 accagtgcca cggcgacggt agtgagcacg cagcaaagat tcaactctca atccctgtca
     1021 tattctccga cgaacgcaac caactctgtg tacctgggag cgagcagcac agactacaat
     1081 atcatagtaa gcccatccac ttcatacttg gtttcttact atgccatggt agccacgggc
     1141 gataccgtag caacggttac tccgacattg acctatgcca ctgcaacaac gcccggtacg
     1201 ctagcgggca cgggtacctc gacgcctgcc aacagtacat gggctagata tacctctgtc
     1261 gtggcggttc ctgccaccgc taccggtcca tcaattctgt cattcaagac gaacacggcg
     1321 acgggcacgg tattcataga tggtattcag attgaacaga aactaagcaa ctcttctacg
     1381 gcgtcaacat ggacccctcc cggtgcaaca ttcatagccg gtgacatgat taagaccggc
     1441 tccatccagt ccacagcaac aactgtagta aatagtgtca caataccagt atggtctatc
     1501 ccactgaatg gttctgcgtc ttttgccaat gttcaggtgc gcggaaacgc ggtggtcggc
     1561 gtcaacaatg atgacgacgg aggattcagc cagattgcat ctggcaacta ttccgcagga
     1621 gatgcgggct gggttattcg ctctgacgga tacgccgagt ttagaaacat ggcgtcggat
     1681 tcgattgacg gtggcgccat caggacaaaa actctatccg tagacacact aacatctgga
     1741 acattgagcg atgagattgg cctgggctcg ggcacatcat tgattgctac tggtctgatg
     1801 ggcgagactg tcgggctagg agactatgga ttccttgtga ctggccctca acaaaacgtt
     1861 gttactttta gggcatctag tgcaaacacc agaacactta ccacaagtgt tccgcataac
     1921 ttcgttgcta cagatgttga tggcactcct ggctcgaaga ttagggtctc catcgggtct
     1981 accgattacg atactccacc tggttctgcc tactatgttg acgacgttcc aaccaccaca
     2041 acatttacat acacggcgtc aacgagtctt acggaatcga caactgccgt aacaaatggt
     2101 gttgttcaag gaagtaatcc agacttgata tcaagcagcc cagtatacat tagttttcct
     2161 accgatggca ctcagcccaa cattatttct gggcaattga ccgcagacca agtaacagtt
     2221 actcagggca tgagcattcg tctgtcatct caaatcgagc agggcgcggt gctgacaata
     2281 aactcagtta ttcagccacc atctgcgggt ccagccatga gtcaatcata tagtggagtt
     2341 tcattccaat tgccgacctc gggctacatc actggaatcg ctcgctccac caacggcaac
     2401 cacctatgtc cggtcggtgg ttatagtggt aaagaatgtt gggtaaatga gtatgatgcg
     2461 aatggaaaga aggtggctga acgag
//
LOCUS       DATMTI010001092         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_719137, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001092 DATMTI010000000
VERSION     DATMTI010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719137"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..808
                     /gene="pal"
                     /locus_tag="VN039_17265"
     CDS             98..808
                     /gene="pal"
                     /locus_tag="VN039_17265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWV47749.1"
                     /translation="MKTLPASYLPLILGVIVLGIPACSKKAVRSGGDSQALQEEMAKG
                     GAAKEGTNSSFPDTSFSGREDSNSLRGLDRNPSEERLGGNAGNAGAPGGGHANAMIAK
                     ADSGAAARQLAEIRAEQVASVAAGLRDVFFTYDSFSITDEGRHALSGDAEWARSNPYA
                     QLKIEGHCDERGTSAYNLVLGEKRAKAVRNYLVELGVAPVHLSVVSYGKERPFCTEHT
                     ESCYSQNRRGHLVVKTGK"
     gene            875..2278
                     /gene="ybgF"
                     /locus_tag="VN039_17270"
     CDS             875..2278
                     /gene="ybgF"
                     /locus_tag="VN039_17270"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HWV47750.1"
                     /translation="MTSQRRITHGMLLGIAVGGLFLPGCVAQQSDLKKAEKDLQQQLS
                     QTRARQSQEISTLREHELPQLRGELEKALHQARELQNKQEDFKHRSAQLEQQTKKLEQ
                     LAAKLETDSSTRYLWMQKSFETQDAKVSARLDELSKAMEALKKEVIDVVQRTNEGLAK
                     RVDGKLEGHQKELAEHQNKIEQISQKFTQFNQALTGFREALTGLNDRVGEYEQTAKHL
                     TSRIDADSKTTTTHAEDVNRSVISITKALEAVGKKVTVRLDEQDNRIDALAKTLEQVS
                     NRPAPLQQVHKPMQHSLSGSDEPTLGGGGETAKLSPGPEATGSATTIVPPQESPKFEP
                     AQASADPPDRVRYERVLGQFRDGDLEGARQGFTGFLEDYPNSNLAPNARFWLGESYFG
                     KKEFQRAIDAYDKVEIDYPGSEKIPAAILKKGYAYLALKDRKRASSAFKQVVTLYPKT
                     TEAGKASDKLAQLKEVR"
BASE COUNT          603 a          548 c          680 g          451 t
ORIGIN      
        1 tggtccccgg catcgtaaat atccaggaag gcattgacgg caatcgtcct tcgctgtaag
       61 aaaaagggac gatctttcac aggagggaag gaatccaatg aaaacgttac cagccagcta
      121 cttgccgttg attctcggtg tcatcgtact gggaattcca gcgtgctcga agaaggcggt
      181 tcggtcagga ggagatagtc aggcgttaca agaagagatg gccaagggag gagcggccaa
      241 agaagggacg aattcaagtt ttcccgatac gtcgttctcc ggacgtgaag attcaaacag
      301 cttacgggga ttggatcgca atccttcgga agaacggttg ggcgggaatg ccggtaatgc
      361 aggtgctccg ggtggcggcc atgccaacgc aatgatcgca aaggcagatt ccggcgcggc
      421 agctcgccag ttggctgaga tccgcgcgga gcaagtggct tcagtggcag ccggacttcg
      481 ggatgtcttt ttcacgtacg acagtttttc gatcaccgat gaaggacgtc atgccctttc
      541 cggcgatgcg gaatgggcca gatcgaaccc gtacgcacag ctgaaaatcg aaggacattg
      601 tgacgagcgc ggcacatcag cctataattt agtgctgggt gaaaaacgtg cgaaggccgt
      661 tcggaattat ctcgtcgagc tgggagtggc ccccgttcat ttgtcggtcg tttcctacgg
      721 caaggaacgg ccgttctgca cggaacatac ggaatcgtgt tactcgcaga atcgccgcgg
      781 gcatctcgtc gtcaagacag ggaagtagtg cgctggtcgt gccgttcgcc tgaacgagac
      841 ctgattatcg tcggtcgttt ggagatcagg acatatgacg tctcaacgac gaatcacgca
      901 tggtatgctc ctcggtatcg cggtaggcgg tctcttcctg ccggggtgtg tcgctcagca
      961 gtccgacctg aaaaaagccg aaaaagatct tcagcaacag ctgtcccaga ctcgagccag
     1021 gcagagccag gaaatttcga ccttacgcga acatgaactg ccgcagctgc gaggtgagct
     1081 ggaaaaggct ctgcaccagg ctcgagagct ccagaacaaa caggaagatt ttaagcatcg
     1141 gtcggcccag ctggagcagc agacaaaaaa gctggaacag ttggcggcca agctggaaac
     1201 cgacagcagt acacgttatt tatggatgca gaagagcttc gagacgcagg atgcgaaggt
     1261 gtcggcccgg ctcgatgagt tatcgaaggc catggaggca ttgaaaaaag aggtcattga
     1321 cgtcgttcag cgcacgaacg aagggttggc aaaacgtgtc gatggcaagc tggaagggca
     1381 tcaaaaggag ttggcggagc accaaaataa aatcgagcag atctctcaaa agttcactca
     1441 attcaatcag gcgctgacgg ggtttcggga agcgctgacc ggtctgaatg atcgtgtggg
     1501 tgaatatgag caaaccgcca agcatctcac ctcgagaatc gatgccgatt caaaaacgac
     1561 gacgactcat gcggaggatg tcaatcgaag cgtgatttcc atcacgaaag cgcttgaagc
     1621 tgtcgggaaa aaagtcactg ttcgccttga cgagcaagac aaccgaatcg atgccttggc
     1681 gaaaactctt gaacaagtat caaataggcc ggctcctctg caacaggtgc acaaaccgat
     1741 gcaacactcc ttgtctgggt cggatgagcc cacattgggt ggaggagggg aaacggcaaa
     1801 gttgtcccct gggccggagg cgacgggaag tgcgacaacg atcgttcctc ctcaagaatc
     1861 gccgaaattt gaaccggctc aggccagcgc cgaccctcct gatcgggttc gttatgagcg
     1921 ggtgttaggc cagtttcggg acggggattt ggaaggcgct cggcaaggat ttacaggatt
     1981 tctcgaagac tatccaaact caaacctcgc gccgaatgca cgcttttggc tgggggagtc
     2041 gtactttggc aagaaagaat ttcagagagc gatcgatgcc tacgataaag tggagatcga
     2101 ctatcccggc agtgaaaaga tacctgctgc aatcttaaag aaaggatatg cgtacttggc
     2161 gctaaaagat aggaagcgag cgtcgtccgc ctttaagcag gtggtcacgc tctacccgaa
     2221 aactaccgaa gcgggaaaag cttcggataa actggctcaa cttaaggagg tgcggtaaca
     2281 ca
//
LOCUS       DATMTI010001093         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_508219, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001093 DATMTI010000000
VERSION     DATMTI010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508219"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            694..>2138
                     /locus_tag="VN039_17275"
     CDS             694..>2138
                     /locus_tag="VN039_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWV47751.1"
                     /translation="MNPTTTDTPLEARTPFAADELPLMDAYWRACNYLSLGMLYLREN
                     PLLREPLRVAHIKKRLIGHWGSAPGQNFIWVHLNRVITRYDLNMIYIAGPGHGAPAML
                     ANAYLEGTYSEVYPDKSQDVEGLRRFFKQFSFPGGIGSHATPETPGSIHEGGELGYSL
                     SHAFGAAFDNPDLLVAAVIGDGEAETGPLMASLHSNKFLNPIRDGAVLPILHLNGYKI
                     ANPTISARISSEELHDTFASFGWTPHLVDGDDPPVMHQQMAATVETCINQIRAVQEEA
                     RRSGKAVRPRWPMIILRTPKGWTAPKELDGHKLEGYWRAHQIPILDVVENPAHLAMLE
                     TWMRSYRPEDLFDENGSLVQQLRALAPIGRRRMSANPHANGGLLRIPLRIPDFRTYGV
                     SVIKPGAIEVQSTFLLGQFLRDVMRDNMHNFRVFGPDETASNRIAEVIKGTGKTWLAE
                     TLPVDEDLSQSGRVMEVLSEHLCQGWLEGYLL"
BASE COUNT          479 a          589 c          630 g          440 t
ORIGIN      
        1 ggtgattctc agcggcgtgg tgccaacctc ggagcacaaa gctcgcgcgg agcagctggc
       61 aggacaggta aacggcgtca ttcacatcag caacaatctt caggtccgga acgccaagca
      121 gcagtaagcg catccggcat gatcacgaga cagcccgatg ccgacagaac tagcccctcc
      181 acgtggagga ccatgagaag ttgagaatcg ggccgtcgac gacctgcata ggcccaggat
      241 ctgagaagag acgttgtgga gttattcacg cagttggaag agcagtcgga tgtagtacgg
      301 ctgtgcctga tgaatctaag aggcactgag gggctaggtt catcgtaatt cccatgaagc
      361 gcgtctgacc ttgagcgcgg aaggaggacg atcatgcgtt cggtcatgtg tggacttgcg
      421 atggcggtta cgatcggaat gatgcctgta gaagcctgac gaacgaggtt ttttggcatt
      481 taagtcaaac aatcagagct gttgtctcag ttcgatattg ggtataacca tagaagggca
      541 caattccaca gttcgctgag tcaggctcct tcctgaaggc agtcatttag aggggagacc
      601 atctaactga cagaggcttg gagtctggat cggccctgcc gcgcgatacc gtagcccgcg
      661 ataccctagc catggatgag gagggccttc atcatgaatc caacaacgac cgacaccccg
      721 cttgaagccc gtacgccgtt cgccgccgat gagctgcccc tgatggatgc ctactggcgc
      781 gcgtgtaatt atctttcgct cggcatgctc tacctgcgcg agaaccctct gctgcgagaa
      841 ccgctcaggg tggcgcacat caaaaaacga ttgatcggac attggggttc cgctcccggt
      901 cagaatttta tctgggtcca cctgaaccgg gtgatcacac gctacgactt gaacatgatc
      961 tacatcgccg ggcccgggca cggtgcgcct gcgatgctcg cgaacgcgta tctggaaggc
     1021 acctattccg aggtgtatcc ggataagagt caagatgtcg aagggctgcg cagattcttc
     1081 aagcagtttt cgtttccagg agggatcggc agccatgcca ctccggaaac gcccggttcc
     1141 atccatgagg gcggcgaatt agggtatagc ctgtcgcatg cgttcggggc ggcgtttgat
     1201 aacccggatt tgctcgtcgc cgccgtcatc ggagacggtg aagctgaaac cggcccactg
     1261 atggcctctc tgcattccaa caaatttctg aatcccattc gcgacggcgc cgtgctgccg
     1321 attcttcatc tgaatgggta caagatcgcc aatccgacca tttcggctcg catctcatca
     1381 gaagaattgc acgacacgtt cgccagcttt gggtggactc ctcacctcgt cgatggggat
     1441 gacccaccgg tcatgcatca gcagatggcg gcgactgtcg agacctgcat caatcaaatc
     1501 cgcgccgtcc aggaggaggc gcgccgcagc ggcaaagccg tacggcctcg ctggcccatg
     1561 atcattctcc gcacacccaa aggatggacc gctcccaagg agttggacgg gcacaagttg
     1621 gagggttatt ggcgtgcgca ccagattccg atcctcgatg tcgtcgagaa tccggcgcac
     1681 ctcgcgatgc ttgaaacatg gatgcggagt tacagaccgg aagatctgtt cgatgaaaac
     1741 ggctcactgg ttcagcagct gagggcattg gccccgatcg gccgccgacg gatgagcgcc
     1801 aatccccacg cgaacggtgg cctgctgcgg attccacttc gaattccgga ctttcggaca
     1861 tacggggtgt cggttatcaa gccgggggcc attgaagtcc aatcaacatt tcttttgggt
     1921 caatttctgc gcgacgtcat gcgcgacaac atgcataact tccgggtctt cggaccggat
     1981 gaaacggctt caaaccgcat cgcggaagtg attaagggga ctggtaagac ctggctggcc
     2041 gaaaccctgc cggttgatga ggatctctcg caaagcggac gcgtaatgga agtgctgagc
     2101 gagcacctgt gccagggatg gctcgaaggc tatctgct
//
LOCUS       DATMTI010001094         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_550827, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001094 DATMTI010000000
VERSION     DATMTI010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550827"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1576)
                     /locus_tag="VN039_17280"
     CDS             complement(137..1576)
                     /locus_tag="VN039_17280"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWV47752.1"
                     /translation="MDTKTAAELKSFLEIPYDDLEDMNLKAAQRAEQVDAKDLEQEYT
                     AWLKKEKHIKAVTLCFSDIEGRLHMLDYDKKFLMDSLGNLTFDGSSIRGFTPQHESDL
                     RLAVDWSSIRYFPADVIGAGKVIFFASVLNSDRTPYHSDFRGMLKSFTEHLKKNESIT
                     AYAACEIEGFLVEGQNAEQRYGEHGLNLISTGGYYHSLPMDPLRQFIDKSAEAQRALG
                     FKNEKDHPEVAPSQFEMNFSYADAVRAADQVQLYKLICRQVARSMGHTATFLPKPFVG
                     INGSGMHTNFSLSKNGKNMFYDVKGKDGLSDIAWDFILKLLNHAPEICLVFNPSVNAY
                     RRLDPHFEAPNQIKVSAIDRGSMIRIPMANEKTARIELRSVAPDANPYLVLYTMLKTG
                     FEGERLEKKATADDRARFLPSSINDAIALFDESKFVADILGEDSKRKYSSFKQLVADR
                     SPKELGMIVKASEVLFHHEVTNQMLWNRF"
     gene            1983..2540
                     /locus_tag="VN039_17285"
     CDS             1983..2540
                     /locus_tag="VN039_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47753.1"
                     /translation="MRTFMIACGWMMVFFAGCVPVTDGFRHDLPTKDVLTIVWGDDVS
                     AVGMATTWLQKRGLFVLERSLLYANLETDNLELTHTLKDEAAILQTAKKMGVQEVVFL
                     DRGGDSRAPMISVRGVEVQTGRVSWSGSARYATFETRPSKDALALLTCEALATVWGYR
                     PAGTKWFLSSEKMCAVDGALPTGSR"
     gene            2627..3265
                     /locus_tag="VN039_17290"
     CDS             2627..3265
                     /locus_tag="VN039_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47754.1"
                     /translation="MNATDQNMRRKVDERRLPAGPRSWVLLCSVSLMLGVGGCSLSYD
                     TTNTPRAATEQLVLAQAFQRSLIDAVVPVVRPGQSVAVETTGLTTDQGYASALIERWL
                     NREGFHVPKDGKETLVVRVTLDAFGTLHNQTFFGMPAFSSAFIPFALPQLSIYQASRQ
                     RGLARFSMDFLDKTTGRLVGSTPTYEGDAYYNDYIFLFVFSTTLSDLVPPVP"
BASE COUNT          730 a          905 c          959 g          787 t
ORIGIN      
        1 cgctatgggt atctggttct tctattggtt gaacggtatt caatgcccct gctgaccgat
       61 ataggcccca acggccgacc ttacctaccc ttctcctgtc gtcacacgac tgacccgccg
      121 gtaaggcaaa cgtcggctag aaccgattcc agagcatttg attggtgact tcgtggtgaa
      181 agagaacctc cgaagccttc acgatcatgc cgagctcttt cggagatcga tccgccacta
      241 gctgtttgaa gcttgaatat tttcgcttgc tgtcttcgcc gagaatatcg gcgacaaact
      301 tcgactcatc gaatagcgcg atggcgtcat tgatactact cggaagaaat cgagcacggt
      361 cgtccgccgt cgctttcttt tccaagcgtt ccccttcaaa gccggtcttg agcatcgtgt
      421 acagcacgag gtagggattc gcatcgggag cgaccgaccg cagttcgatg cgcgccgttt
      481 tttcattggc catcggaata cggatcatgg agccacgatc gatcgccgag accttgatct
      541 ggttcggtgc ctcaaaatga ggatctaggc ggcgatacgc attcaccgac ggattgaaca
      601 ccagacaaat ctccggcgcg tgattcagga gcttgaggat gaagtcccac gctatatcgg
      661 agaggccgtc cttccccttc acatcgtaga acatattctt gccgttcttg ctcaaggaga
      721 agttggtgtg catgccggat ccgttgatcc cgacgaacgg cttcggaaga aacgtggcgg
      781 tgtgtcccat ggatcgagcc acttggcggc agatcaactt gtacagttgc acctgatcgg
      841 ccgcgcgtac tgcatcggca taggaaaagt tcatttcgaa ctgcgagggc gcgacctcgg
      901 gatgatcttt ttcgttcttg aaccccagtg cgcgctgcgc ttccgccgac ttgtcgataa
      961 actggcgaag cggatccatc gggagtgagt ggtagtatcc gccggttgag atgagattca
     1021 gtccatgctc accgtatcgt tgctcggcgt tctgcccttc caccagaaag ccttctattt
     1081 cacaggcagc gtatgccgtg atgctctcgt ttttcttgag gtgctctgta aacgatttca
     1141 gcataccgcg aaagtcgcta tgatacggcg tacgatcgct gttcaagaca gacgcaaaga
     1201 aaatcacctt tcccgccccg atcacgtccg ccggaaagta gcggatggat gaccaatcga
     1261 cggcaagacg cagatccgac tcatgttgcg gcgtgaaccc acggatggac gatccgtcaa
     1321 acgtcagatt tcccaacgag tccatcagaa acttcttgtc gtaatcgagc atgtggagac
     1381 gtccctcgat gtcggagaag cacaacgtca cggccttgat gtgcttttcc tttttgagcc
     1441 aggccgtata ttcctgctcg agatcttttg cgtcgacctg ctcggcgcgc tgcgccgcct
     1501 tgagattcat gtcttcgaga tcgtcatacg gaatctccag aaaactcttc agttcggccg
     1561 ctgtctttgt atccatggtt cacctcacca attcgtagta tccaaggatg gacttatgca
     1621 ctcagggctc atatcgcctg aatgcttgtt gccttcacga ccacctttac cagagacgag
     1681 caagaagcga tcatccggcg gaaacgagtc tggcacacct tgcttacgca tgcaatgtgg
     1741 atttggagga atgagcgtgg tccatgtggg gtcttgcatg tgccttcgtc gtcttcttgc
     1801 gtgcattggc acgcacagaa caagatatga ctaattcagc aacgttctga ttgcacatag
     1861 ccatcgctat gtgtaggatc ggacacagaa gcgtcgcttt tttggaatcg gaagtctggg
     1921 gctttctcga ggcgaccgag gaaattgaac cgtcggatac tgagagttgt acggagggtt
     1981 tcatgaggac attcatgatc gcgtgtggct ggatgatggt attcttcgcc gggtgcgtgc
     2041 cggtgaccga cgggttccga cacgatctgc cgacgaagga cgtgctcacg attgtttggg
     2101 gagacgatgt gagtgccgtg ggcatggcga cgacctggct gcagaaacga gggctgtttg
     2161 ttctcgagcg gagcctcttg tacgcgaacc tggagacaga caacctcgaa ctgacgcaca
     2221 cgctcaagga tgaagcggcc atccttcaga ccgcgaagaa gatgggagtg caggaagtgg
     2281 tatttctcga ccgcggaggc gattctcggg ctccgatgat cagcgtccgg ggcgtagagg
     2341 tccagaccgg acgagtctcc tggagcggca gcgcccggta tgccacgttc gagacccgtc
     2401 cctcgaagga tgccctcgct ctgctgacgt gtgaggcgct cgcgacggta tgggggtacc
     2461 gtccggcagg aacaaagtgg ttcctctctt ccgagaagat gtgcgccgtt gatggagctc
     2521 tcccgacggg atcgcgatga gttggctcgg acgattcata agaggcagca gcaagagagg
     2581 cggatcatcg atccaccttc cgatgaaccg tgtgaaaggt cttcgcatga acgcaactga
     2641 tcaaaacatg aggcgcaagg tcgacgagcg gcggctgccg gccggaccgc ggagctgggt
     2701 cttactctgc tcagtgagct tgatgctggg agtcggaggg tgttcgctct cctacgacac
     2761 gaccaacacg cccagggccg ccaccgaaca attggtcttg gcccaggcgt tccaaaggag
     2821 tctgatcgat gcggtggttc ctgtcgttcg tccaggacaa tctgtcgcgg ttgagacgac
     2881 cggccttacg accgatcagg ggtatgcgag tgcgttaatt gagagatggc tgaaccggga
     2941 agggtttcat gtgccgaaag acggaaaaga aacactggtg gtcagggtga cgttggacgc
     3001 gttcggcacg cttcacaatc agacgttctt cggcatgccg gcgttcagca gcgcgttcat
     3061 cccatttgcc ttgccccaat tgtctatcta ccaggcgtcc aggcagcgag gacttgcccg
     3121 tttctcgatg gattttctgg ataagacaac cgggcggctt gtaggctcga ctccaacata
     3181 cgagggcgat gcctactata acgactacat atttctgttc gtcttctcga cgacattgtc
     3241 ggatctagtg ccgcctgtcc cctagatgca atcaatcatg cggtgatctc cacccacgtc
     3301 cctcctacaa atgaaagtat gtaccacggg tatagcacca tctgagtgta acattgacga
     3361 agggaggagg ctcggattca c
//
LOCUS       DATMTI010001095         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_338432, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001095 DATMTI010000000
VERSION     DATMTI010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338432"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1855
                     /locus_tag="VN039_17295"
     CDS             74..1855
                     /locus_tag="VN039_17295"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWV47755.1"
                     /translation="MRMWLVLGALVWSIWLVPPVRAEDQPQTLAFAQVAVPTGGLPPA
                     TLLVEPVPPVANPCPRFAPGAVVHQPPALFSRDGVLAIRLSYQTRTDFANRALFCFMT
                     PTGHENPTLHVKPGDHVIITVTNNTPAQAGHMTINPPHCGPGGALMTGSSLNMHFHGT
                     NTSPTCHADEVIKTLINSGQTFQYRLTIPADEPPGLYWYHPHVHALAEQALLGGASGA
                     LVVDGIENLKPAVAHLRQRILMIRDQPTRQGLPEGAGGDRHGVPFQDLTINHVPTDAT
                     TDPTNHTTYTPAILHMEAGERQFWRISNSTADSILDLQVQFDGIPQTMDVVAIDGVPV
                     NSQDGAQPGSLIPVTHFRLPTASRVEFIVHAPSSSVNLAQLVTRNIQTGANGDDDPHR
                     PLLDIHVRSHLHDEPAEDDRVGHFGAYNPHLTRFAGLTAAPVATRRLVFFDEIQPTQF
                     FMVVDGQPAKVFDPNAGPAITATQGTVEEWTVENHALENHEFHLHQLHFLVEAQSNFE
                     INGNEPAPGITGQFLDTIEVPHWDGDPAHPYPRVTLRIDFRGKDIGDFVFHCHILNHE
                     DLGMMNIIRVQRKSSSSSDLIERALGG"
BASE COUNT          416 a          600 c          506 g          395 t
ORIGIN      
        1 agagactaaa ctgtccaatt cttgccaggc cagagacccc gagtccagag ccacaaccga
       61 aaggagcatc tcgatgcgaa tgtggcttgt gctcggagcg ttagtgtgga gcatttggct
      121 ggtcccacca gtgagggcag aagatcagcc tcagacgctt gcctttgccc aagttgccgt
      181 gccaactggg gggcttcctc ctgccaccct tctcgtggag ccagttccac cggtggccaa
      241 tccttgtccc cggtttgccc ctggggcggt cgtccatcag cctccggcgc tctttagtcg
      301 agatggcgta ttggccatcc gattgtccta tcaaacccga actgactttg cgaaccgagc
      361 gttgttttgc tttatgactc cgaccggtca cgaaaaccct accctgcatg tgaagccggg
      421 tgatcatgtc atcatcaccg tgaccaacaa tacgccggca caagcggggc acatgacgat
      481 caatccccca cactgcggcc ccggcggtgc cctgatgacg ggatcatcgc tgaacatgca
      541 ctttcatggc accaatacgt cccccacctg ccatgccgat gaagtgatca agaccctcat
      601 taattccggc cagacgttcc aatataggct cactattcct gccgacgagc cacctggtct
      661 gtattggtat catccccacg tgcacgcgct ggccgaacaa gccctccttg gtggcgcttc
      721 cggagccctg gtcgtggacg gcattgaaaa cctcaaaccg gcggttgcgc atctgcgtca
      781 gcgcatcctc atgatacggg accagccgac gcgccagggc ttaccagaag gcgcaggggg
      841 agaccgccat ggcgttccct tccaggacct cacaatcaac catgttccga cagacgccac
      901 gactgacccc accaatcaca ccacctatac ccctgccatc ctccacatgg aagcggggga
      961 gcgccagttc tggcgtatat ccaattctac ggcggacagc attctcgatc tccaagtgca
     1021 gttcgacggc atcccgcaaa ccatggacgt cgtcgccatt gacggggtac cggtgaactc
     1081 tcaggatggg gctcaacctg gcagcctcat tccggtgaca cacttccgac tgccaactgc
     1141 gtcgcgggtg gaatttatcg tccatgcgcc ttcgtccagt gtgaatctcg cacaattggt
     1201 gacccggaat atccaaacgg gagccaacgg cgacgatgac cctcaccggc ccctcttgga
     1261 tattcacgtg cgttcccacc tacacgacga accggctgaa gatgaccgtg tcggccactt
     1321 cggcgcgtac aatccccacc tgacccggtt cgccggtctg actgccgcgc ccgttgccac
     1381 acggcggctg gtgtttttcg atgagatcca accgacccag ttctttatgg tggtggacgg
     1441 acaaccagca aaagtctttg atccgaatgc aggccctgcg attacggcga cccaaggcac
     1501 ggtggaagag tggacggtgg aaaatcatgc gctggagaac cacgaatttc acttgcacca
     1561 gctgcacttc ctggtggaag cgcagagtaa ctttgaaatc aacggcaatg agccggcgcc
     1621 ggggatcacc gggcaattcc tcgacacgat cgaagttcca cactgggatg gagatccggc
     1681 ccatccgtat ccccgtgtca cacttcggat cgactttcgt ggaaaggaca tcggtgattt
     1741 tgtgtttcat tgccacatcc tgaaccacga ggatttgggc atgatgaaca ttatccgcgt
     1801 ccagcggaaa tcctccagct cgagcgacct gattgaacga gctctagggg gctaaccagt
     1861 ttctgaacag gaatggagca gcttgcaaac gtgcttcact agagcttcag gcattgc
//
LOCUS       DATMTI010001096         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_338440, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001096 DATMTI010000000
VERSION     DATMTI010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338440"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN039_17300"
     CDS             <1..322
                     /locus_tag="VN039_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV47756.1"
                     /translation="AAWCKLGEHQFMAIFEVEQLQPDRTKHFGLMVRDAKQIQEVRKK
                     LTKKYKLKLHPDFRCDFRDPWGNRIQVGDLSDESLVWLLPYQEVQKAGIVFTSKQNMK
                     GVRR"
     gene            319..690
                     /locus_tag="VN039_17305"
     CDS             319..690
                     /locus_tag="VN039_17305"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWV47757.1"
                     /translation="MSDHNLQQRQNDLFGYVRQGKIIEAMNEFYDKDTVMQDNANPPT
                     KGLAANIEREKQFLSGVKEWKGFEVKAGGTGDNVTFYECTIDFISTSGQPVHMEQVSV
                     AKWKNGKIVHERFYYDTGAKR"
     gene            892..2496
                     /locus_tag="VN039_17310"
     CDS             892..2496
                     /locus_tag="VN039_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HWV47758.1"
                     /translation="MDSLHQLEIIILLLAIVLALTTVARRILIPYPILLVVGGLVLGV
                     VPGLPTVGLNPDLVFLVFLPPILWAAAYFTSWRDFRQNIRPISLLAVGLVLATTAAVA
                     GVAHAVLGFGWAEAVALGAIVSPPDAVSATAIGKRLNMPRRIVTILEGESLVNDATAL
                     VLYRAAVAAVVSGGFVVGEALFQFVYAAVAGVAVGILVGWVTRWALHAIGESFTEIAA
                     TLLAPYVAWVIGESIHASAVLSCVAGGIYLRQHFSKVVAPTTRLQGRAVWDLVVFVLN
                     GVIFILIGLQLGTLWDAVPSDRLWSLVIGGTLISVAAIVVRLGWVPLIATIPRYLSST
                     LRARDPLPPWPHIFLVAWTGMRGIVTLAAAMALPVSIGTGAPFPFRSEIILLSFMVIL
                     ATLVLQGLTLAPLIRTLGVSGGSEGEEEEMHAREQAAGAALVRLNELTKENWPKPAHV
                     EQLELHYGGRLRRFSPDAEIDPDCKKETSEAFLRLREATLAAERSALIRLRDEGTISD
                     EVLHRLEHEVDVEALRLGIGERRMHV"
     gene            2566..3357
                     /locus_tag="VN039_17315"
     CDS             2566..3357
                     /locus_tag="VN039_17315"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV47759.1"
                     /translation="MTQAQHQASIIQQFTRQAGPFAAMPEHSQESAFRLMLESTGVTS
                     SDTVLDVACGPGLVACAFAARAAWVTGIDITPAMIDKARRLQEDKGITNVEWNNADVL
                     PLPYADESFSTVITRYSFHHFLDPFRVFMEMHRVCKRNGVLMVVDVAIPSEKRAAYDH
                     LEKLRDPSHTSACTPEELLGMAERLKLARVTTQWYRLAMELERQLAASFPRPGDDETI
                     RALLRNDIGQDHLGMGAHWVGTEIHFVYPTLILVGRKGGRYSQWR"
     gene            3360..>4204
                     /locus_tag="VN039_17320"
     CDS             3360..>4204
                     /locus_tag="VN039_17320"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV47760.1"
                     /translation="MSGYRWALSVLAVGSLLLQGPGLSAQQFRPDDEITKLAGDVYLY
                     RHQFHQAIFITTPKGVIVTDPISSDAATWLKAKIKTLTDQPVRYVIYSHHHDDHITGG
                     SVFADQASFVSHAAAREKILQAEDPKTPVPGLTFTDRMSIDLGGKHVELIYTGKNHSD
                     NSLVVLLPQNKLLFAVDFIPVETVAYRTMASDYPDDWIESLKRVEQLDFETLVPGHGR
                     IGKKEHVRQFRGYLEDLRAAVEEQVRQGASVEEVKKTVQLPKYEQWQLYADWFPENVE
                     GMYRYL"
BASE COUNT          900 a         1171 c         1253 g          880 t
ORIGIN      
        1 ggctgcctgg tgcaagctcg gcgagcatca gttcatggcc attttcgaag tcgagcaatt
       61 gcagccggat cgcacgaagc attttggcct catggtccgc gatgcgaagc agattcagga
      121 agtccgcaag aaactgacga agaagtataa gctgaagctt catcctgact ttcgctgtga
      181 ctttcgggat ccctggggca atcggattca agtcggcgac ctcagcgatg aatccttggt
      241 gtggctgctg ccgtaccaag aagtgcaaaa agcgggaatt gtatttacct ccaaacaaaa
      301 catgaaagga gttcgacgat gagcgatcac aatctgcaac aacgacagaa cgacctattc
      361 ggctacgtcc gccagggcaa gatcattgaa gctatgaacg agttttacga caaggacacg
      421 gtaatgcaag acaatgcgaa tcccccgacg aagggcttag ccgccaatat cgagcgagaa
      481 aagcaatttc tgagcggcgt gaaggaatgg aagggctttg aagtgaaagc cggcggcacc
      541 ggtgacaacg tcacgttcta tgaatgcacg atagatttca tctcgacgag tggacaaccg
      601 gttcatatgg agcaagtctc cgtcgcaaag tggaagaacg gcaagatcgt ccatgagcgg
      661 ttctattacg atacgggcgc aaaacggtga cttcaatcgg cgccgatcac ggcggtcgag
      721 ttcaccccgc ctaggcagga agcctcggtt gtgaagccgt ggaggaatgc gcaacgagtg
      781 tcctccgaag ccttaggcta aggaggacac atgtcggccc gataacggga ctcgatggag
      841 gaatgggatt gatgggaaac ggacgctgta actaaccggt gttgtatcaa gatggacagc
      901 ctgcatcagc tcgaaatcat catcctgctg ctcgcgatcg tgctggccct cacgaccgtg
      961 gcgcgcagga ttttgatccc ctatcccatt ctacttgtcg tcggcggact cgtattgggc
     1021 gttgtgccgg gattgccgac cgtcggtctg aatcccgatc tggtcttttt ggtcttcttg
     1081 ccgccgatct tatgggcggc cgcttacttc acttcgtggc gcgattttcg acagaacatc
     1141 cgcccgatct cgctcttggc ggtggggctt gtactggcga ccacggcggc tgtcgcggga
     1201 gtcgcccatg ctgtgttggg gttcggttgg gctgaagccg tcgcactcgg cgccatcgtt
     1261 tctcctcccg acgccgtatc ggccacggcc atcggcaaac ggctcaacat gccgcgccgg
     1321 atcgtcacaa tcctggaagg cgaaagcctt gtcaacgacg caaccgcatt agtcctctat
     1381 cgggccgccg tcgcggcagt cgtcagcggc ggtttcgtgg tgggagaggc gctgttccaa
     1441 tttgtgtatg ctgcggttgc gggcgtcgcg gtcggaattc tggtcggatg ggtgacgcga
     1501 tgggcgctcc acgccattgg ggagagcttt accgagatcg cagcgacgtt attggctccc
     1561 tacgtggctt gggtaattgg tgaatcgatc catgcctccg ccgtgctgtc ctgcgttgct
     1621 ggaggcatct atcttcggca acacttcagc aaggtggttg cgccgacgac ccgacttcag
     1681 ggacgtgccg tgtgggattt ggtcgtattc gtgctgaacg gtgtgatttt catcctgatc
     1741 ggcttgcaac tcggcacgct ctgggatgcc gttccgtcgg atcgactgtg gtccctcgtg
     1801 atcggcggga cactcattag cgtggcggcc attgtcgttc gattgggatg ggttcccttg
     1861 atcgcgacaa tcccccgata cctgagttca acacttcgtg cccgggaccc tctgccgcca
     1921 tggccgcata tttttttggt ggcctggacc ggcatgcggg gcattgtgac cttggctgcc
     1981 gcgatggctt tgcccgtctc aatcggcacc ggcgcaccct tccctttccg gtcggaaatt
     2041 attctgctta gctttatggt gattcttgcc acattggtcc tccaggggtt gacgttggct
     2101 ccgttgatac gaacactcgg agtgagcggc ggctcggagg gagaggaaga agaaatgcat
     2161 gcgcgggaac aggcggccgg cgcggcactg gtgcgcctga acgaattgac taaagaaaac
     2221 tggccgaaac cggctcatgt cgagcaattg gaattgcatt atggcgggcg tctccggcgc
     2281 ttctcgccgg atgccgagat cgatcctgat tgtaagaaag aaacgagcga agcctttctt
     2341 cgactcagag aagcgacctt agcagccgaa cgttcggcat tgatccggct ccgggatgag
     2401 ggcacgatca gcgacgaagt gttgcaccgc ctggaacatg aagtggatgt cgaagcattg
     2461 cgcctgggga tcggcgaacg gcggatgcac gtttagcgat catactcaac cagtccgtcc
     2521 catttttcac agaaacggtc ccgcgttgag taaataggag acaggatgac acaagcacaa
     2581 catcaggcat ccatcatcca gcaattcacc aggcaagccg gtcccttcgc ggccatgccc
     2641 gaacattcgc aggagtcagc cttccggctc atgcttgagt cgaccggcgt cacgtcgagc
     2701 gataccgtac tcgacgtcgc ctgcggcccg ggcctggtgg cctgtgcctt cgccgcgcga
     2761 gccgcatggg ttacgggaat cgacatcacg cctgccatga tcgacaaggc gcggcggctc
     2821 caagaggaca aagggatcac gaacgtggaa tggaacaacg ccgatgtcct gccgctgccc
     2881 tatgcagacg aatccttttc cacggtgatc acgcggtact cctttcatca tttccttgac
     2941 cccttccggg tgttcatgga aatgcaccgg gtatgcaaaa ggaacggagt gttgatggtc
     3001 gtcgatgtcg cgattccgtc ggaaaaaaga gcggcttacg atcatcttga gaaactgcgg
     3061 gatccgtccc acaccagcgc ctgcacgccg gaagaactgc tcggcatggc cgaacggttg
     3121 aagctcgctc gcgtcacaac ccagtggtac aggctcgcga tggaactcga acggcaattg
     3181 gctgcgtcgt ttcctcgtcc cggcgacgac gagacgatcc gcgcactctt gcgcaacgat
     3241 atcgggcaag atcatctcgg gatgggcgcc cattgggtcg gcacggaaat ccatttcgtc
     3301 taccccacac tcatcctggt cgggaggaag ggtggacgat acagtcaatg gaggtaacca
     3361 tgagtggata tcgttgggct ctgtcggtgc tcgccgtcgg aagtctgctg cttcaaggac
     3421 cgggattgtc cgcccaacag ttccggccgg acgatgaaat caccaagctg gccggggacg
     3481 tctatctgta ccggcaccaa tttcaccagg ccattttcat cacgacgccg aagggcgtga
     3541 tcgtgaccga tccgatcagt tccgatgcgg cgacctggct caaggccaag atcaagaccc
     3601 tgaccgatca accggtccgt tatgtgattt acagccacca ccacgacgac catatcaccg
     3661 gcggcagcgt cttcgccgac caggcgagct tcgtcagcca tgcggcggcg cgggaaaaaa
     3721 ttctccaagc ggaggatccg aaaacacccg tccccggcct caccttcacc gaccggatgt
     3781 cgatcgacct cggggggaaa catgtcgaac tgatctatac cggcaagaac cattcggata
     3841 atagtctcgt cgtcctgctc ccgcagaaca aactcctctt tgccgtcgat ttcattccgg
     3901 tcgagacggt ggcctaccga acgatggcga gcgattatcc ggacgactgg atcgagtcgc
     3961 tcaaacgagt cgagcaattg gacttcgaga ccctggtccc cggccatggc aggatcggca
     4021 agaaagagca tgtgcgacaa tttcgcggct acctcgaaga cctcagagcg gccgtggagg
     4081 agcaagtccg ccagggcgcg agtgtggaag aagtgaaaaa gaccgttcag cttcccaagt
     4141 acgagcagtg gcaactgtac gcggactggt ttccggagaa tgtggagggg atgtaccggt
     4201 atct
//
LOCUS       DATMTI010001097         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_592246, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001097 DATMTI010000000
VERSION     DATMTI010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592246"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..2967
                     /gene="secA"
                     /locus_tag="VN039_17325"
     CDS             244..2967
                     /gene="secA"
                     /locus_tag="VN039_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWV47761.1"
                     /translation="MVTQVLNLIFGSKNDREIRTLRPIVEQINGLEAGLTPLSDQALA
                     DNTQDFRKRLEAGQTLDEILPEAFAVCREMSRRKLNMRHFDVQLIGGMILHRGRIAEM
                     KTGEGKTLVATLPIYLNALEGKGAHLVTVNDYLAKRDAQWMGQLYHALGLSTGIIQHD
                     ASFIFDPTYEAADKRLQHLRPCTRPEAYRADITYGTNNEYGFDYLRDNLVVTDLNQCV
                     QRELNFAIVDEVDSILIDEARTPLIISGPTDQTTDLYYRINAIIPQLKAETDYTIEEK
                     TKTAALTDDGNARVEKLLGVDNLYDPAHMDLVHHVVKALQAYTLYKRDVDYVVKDGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYKKLSGMT
                     GTADTEAAEFAKIYNLDVNVVPTNRQMIRQDYADVVYRTEKEKFAAIVEDIKECHERG
                     QPVLVGTISIEKSEKLAGLLNRNGVKHNVLNAKQHEREAEIVAQAGRKGAVTIATNMA
                     GRGTDILLGGNPDFMYKQVLYRDETIPEDRKLEIYEGIRSDCDKDKQEVLAAGGLHIL
                     GTERHESRRIDNQLRGRAGRQGDPGTSRFYLSLEDDLMRIFASERVSQLMLKLGMEEG
                     VPIEHGMVTRAIANAQKKVEAHNFEIRKQLLEYDDVMNKQREVIYRHRRAVLNGDNLT
                     NDLHEMMDGSVESSLNVYCPPDQYPEEWDVKGLTEVMQSQFGLDITQDKHDGGDSLRD
                     VGRDALQEDLRAHVREAYAKKEQELGQELMRFLEKTFILQVIDHHWKDHLLAMDHLRD
                     GIGLRGYGQKDPLIEYKREGFDLFAGMMERIKSDTLDRLFHVQAVRHEGEQPSPSPQP
                     VISRPQPKLTLNRGEEPVAAQAPVHRTDNKVGRNDPCPCGSGKKYKKCHGA"
BASE COUNT          791 a          885 c          835 g          577 t
ORIGIN      
        1 actgcccttc aatcaggccc ttcttctggc ctttaccttt actaatgtac ttatgcgttt
       61 ctacacggtg attctacacg catggtgaat cttaggtgag ctgaacaatt tctgtcaagc
      121 caccatgatg ccatccgagg ttcgagatag gattcaccgg agagatcctg tatactattg
      181 tgattatttt gacggcaaac tcatttacag cacgacgaat gcctttctga caaatgcctt
      241 tctatggtta cacaagtact caacctgatc ttcggcagta aaaacgaccg cgaaatcagg
      301 actctgcgac caatcgtgga gcaaatcaac ggcttggagg ccgggctcac accactctcc
      361 gatcaagccc ttgccgacaa cactcaggac ttcaggaagc ggctggaagc aggtcagacg
      421 ctggacgaga tcctgcccga agcgtttgcc gtgtgccggg aaatgtctcg ccgcaaactc
      481 aacatgcggc attttgacgt gcaattgatc ggcggcatga ttctccaccg tggccgcatc
      541 gccgaaatga aaaccggcga aggaaagacc ttggtggcga cgctccccat ctatctgaat
      601 gcgctcgaag ggaaaggcgc tcatctggtc accgtcaacg attatcttgc caaacgtgac
      661 gctcagtgga tgggacagct ctaccatgcc ctgggcctat ccacgggaat cattcagcat
      721 gatgcttctt tcatcttcga tccgacctac gaagccgcgg acaaacggct gcaacacctt
      781 cgtccttgca cgagacccga ggcgtatcgc gccgatatta cctacggaac gaacaacgaa
      841 tacgggttcg attatctccg cgacaacctc gtggtcaccg atttgaacca atgcgtccaa
      901 cgcgagttga acttcgccat cgtcgacgaa gtcgacagca ttttgatcga tgaagcgcgg
      961 acgccgttga tcatctcggg ccccacggac cagaccaccg acctctatta ccgaatcaac
     1021 gcgatcattc cgcaactcaa agctgaaacg gactacacca tcgaagaaaa gaccaagacc
     1081 gccgcgctga cggacgacgg caacgcgcgc gttgaaaaac tgctcggtgt cgacaatctg
     1141 tacgatccgg cccacatgga cctggtccat cacgtggtga aagctttgca agcctacacg
     1201 ctctataaac gagacgtgga ctacgtggtg aaggacggcg aagtcatcat cgtcgacgaa
     1261 tttaccggcc gcttaatgcc ggggcgacgg tggagcgacg gactccatca agccgtcgaa
     1321 gccaaagagg gcgtcaagat cgccaacgag aatcagacgc tcgcgtcggt gacgtttcaa
     1381 aactatttcc gcatgtacaa gaaactcagc ggcatgaccg ggaccgcgga cacagaagcc
     1441 gctgaattcg ccaagatcta caacctcgac gtcaacgtcg tgccgaccaa tcgccaaatg
     1501 attcggcagg attatgccga cgtcgtatat cggacggaga aagaaaagtt cgcggcgatc
     1561 gtcgaagaca tcaaagaatg tcatgagcgc ggccagccgg tattggtcgg aaccatttcg
     1621 atcgaaaagt cggagaagct cgccggcttg ttgaatcgca acggagtcaa acacaacgtc
     1681 ctcaacgcca agcaacacga acgcgaagcc gaaatcgtcg cgcaagccgg gcgcaaaggc
     1741 gcggtcacca ttgccacgaa catggcgggg cgaggcaccg acatcctcct gggcggcaac
     1801 cccgacttca tgtacaagca agtgctctat cgggacgaaa ccatcccgga agaccgcaag
     1861 cttgaaatct atgagggcat tcgatccgat tgcgacaagg acaagcagga agtcctcgcg
     1921 gccggcggcc tccatatcct cggcacggaa cggcacgaga gccgccgcat cgacaaccag
     1981 cttcgaggac gggccggccg ccagggcgat ccagggacgt cgagattcta cctgtcgctg
     2041 gaagacgacc tcatgcggat tttcgcttcg gaacgggtgt cacagctgat gctcaagctc
     2101 ggcatggagg aaggcgtccc catcgaacac gggatggtga ctcgcgcgat cgcgaacgcg
     2161 caaaagaaag ttgaggcgca taatttcgaa atccgcaaac aactgctgga atacgacgac
     2221 gtcatgaata agcagcgcga agtgatctat cgacaccgtc gggctgtgct gaacggggac
     2281 aacctcacca acgaccttca cgagatgatg gacggctccg tggaatcctc cctcaatgtg
     2341 tattgccccc ccgatcaata cccggaagaa tgggacgtga aaggtttgac cgaagtcatg
     2401 cagagtcagt tcggcttgga catcacccag gataagcatg acgggggaga ctcgctgcgc
     2461 gacgttggac gcgacgcatt gcaggaagat cttcgagccc acgtgcggga ggcctatgcc
     2521 aaaaaggaac aagagctagg gcaggagctg atgcgttttc ttgaaaagac attcatactc
     2581 caggtcattg atcaccattg gaaagatcac ctgcttgcca tggaccattt gcgcgacggt
     2641 atcggtctcc gcggctacgg tcagaaagat ccgctgatcg agtataaacg cgaggggttt
     2701 gacctcttcg ccggcatgat ggaacggatc aaatccgaca cgctggatcg gctgttccat
     2761 gtgcaggctg tccgccacga aggagaacaa ccgtcgccct caccgcaacc ggtcatctca
     2821 cgtccacagc ccaagctcac cttgaaccgc ggagaggaac cagtcgcagc gcaagcccct
     2881 gtacatcgga ccgacaacaa agtcggccgc aatgatccct gcccctgcgg cagcggaaag
     2941 aaatacaaaa agtgccacgg ggcgtgaggg gaaatgagct gcggcctggt gcgccatatt
     3001 gcttgccccc acgcataagt ttccttacgg taatgtgtag gtaaggttcg gccaggaggt
     3061 atagccgact gcccgagcct tgccggcc
//
LOCUS       DATMTI010001098         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_381513, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001098 DATMTI010000000
VERSION     DATMTI010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381513"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VN039_17330"
     CDS             complement(<1..409)
                     /locus_tag="VN039_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47762.1"
                     /translation="MTAKQWLIESDRTTDSMTFYGRAVVFVVLVWWGLKFIVTPLETN
                     YTGESFLHLINLPFHEAGHVIFIPFGRFMTILGGTLGQILMPIICLGTFLIKTRDPFG
                     AAVALWWTAESMMDIAPYINDARALDLMLIGGVT"
     gene            complement(661..1641)
                     /locus_tag="VN039_17335"
     CDS             complement(661..1641)
                     /locus_tag="VN039_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWV47763.1"
                     /translation="MGGFGRHEHRDLDRGLVMDAGEERVPQLVDQIESDVDEEPSFFS
                     KWTLPIVLFLLTVFTTLWAGAYQAYTGPVRGPLNFLLASPEMLWRGIPFAGALLFILT
                     THELGHYLLSKIHRVPASLPLFIPGPPHFIGTFGAIIRMRGPILSRHALFDIGVAGPL
                     AGFAVAIVAMIVGLNLSTVVDRTATYGLQLGEPLLLQFMSWVVIGPIPPEADVVLHPI
                     GFAAWFGLFVTSLNLLPIGQLDGGHVAYALWGPRQRTMALAFLPILLVLGFVGWSGWF
                     LWAFMAGLWGVGHPPVMDPHVPLGRNRTIVGWIAFAVFVVTFAPVPFSFH"
     gene            complement(1653..>2553)
                     /locus_tag="VN039_17340"
     CDS             complement(1653..>2553)
                     /locus_tag="VN039_17340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV47764.1"
                     /translation="EGFASFGKPPWSLIGEGSRMSLEDDLSDILKKARTGQGLSVGDV
                     ARMTGLPGGDITALERGDQPRDHAEVRALAKALGLRAEPIEQIAIDKWEPVAQRMPPW
                     VDMVHGSINGYGVQGYVVHDEGEALLVDTAYNAPAMLDLLRRRGMRLVGICLTHGHAD
                     HAEGIEQILDSHEIPVYLGPEDISLLSWRPRTDLLVTPSDGMSISVGRRTVHCVTTPG
                     HTPGGICYRLDEAQHPVCFVGDTLFAGSIGRSNPKELYSTHLNSVRRRVLTLSPDYRL
                     FPGHGPATTVEEEVDHNPFGTIQ"
BASE COUNT          657 a          754 c          692 g          450 t
ORIGIN      
        1 ccgtcactcc tccgatgagc atgagatcca gcgcccgtgc atcgttgatg tagggggcga
       61 tatccatcat gctctccgca gtccaccaga gtgccactgc agccccgaaa ggatcacgag
      121 ttttgataag gaacgtacca agacagatga tcggcatgag aatctggccg agcgttcccc
      181 ccagaatcgt catgaagcga ccgaacggga tgaagatcac atgcccggct tcatgaaagg
      241 gtagattgat gagatggagg aatgactcgc cggtatagtt ggtttccaga ggcgttacga
      301 tgaacttcaa accccaccag accagcacga cgaacacaac ggcacgaccg tagaacgtca
      361 tcgaatcggt cgtccggtcc gattcaatca gccattgctt agccgtcatc aaccatttcg
      421 atctcgcaaa cgcggaggag gagcggagac gggctttccc tgcgcctggt cggtatttgg
      481 caaagatgat gccgcatcgg acacactcct ccgcctcttc caaccgttga gcctgacatt
      541 tgggacaatg ggttgtggac gcaccggcgg taagcattct tctactgtag ggtcgcccgt
      601 gaattcggac aagaaaatca ggagaacggc acaagcgaga aggaagaagt cacgaagcaa
      661 ttaatgaaag gaaaacggca ccggagcaaa cgtgacgaca aacacagcaa aggcaatcca
      721 tcccacaatc gtccgattac gccccaacgg aacatgagga tccatgactg gaggatgacc
      781 cacgccccac aatcccgcca tgaacgccca cagaaaccag ccggaccaac cgacgaatcc
      841 caagaccaat aagataggta gaaaggcgag cgccatcgtc cgctgtcgcg ggccccacaa
      901 ggcgtaggcg acatgtccgc cgtcgagctg accgatggga agaagattga gggaggtgac
      961 aaagagcccg aaccaggcgg caaagccgat cggatgaagc acgacgtcag cctccggagg
     1021 tatcggcccg atcaccaccc acgacatgaa ctgcagcaac aaaggttccc ccagttgcaa
     1081 tccatacgtg gccgttcgat ccacgaccgt ggagagattg aggccgacga tcatcgcgac
     1141 gatggcgacc gcaaatcccg ccaagggacc ggctaccccg atgtcaaaca aggcgtgccg
     1201 actcagaatc ggaccccgca tgcggatgat ggcgccgaat gtcccgatga agtgaggagg
     1261 cccgggaatg aacaacggca aggaagcagg cacccggtgg atcttggaca agagataatg
     1321 ccccaactca tgcgtcgtga ggataaagag caacgctcca gcaaagggga ttcctcgcca
     1381 aagcatctca ggagaggcca gcagaaaatt cagaggcccg cgcaccggac cggtgtaggc
     1441 ttggtacgcc cccgcccaca gcgtggtaaa gaccgtcagg agaaagagga cgatcggtaa
     1501 cgtccacttc gagaaaaacg acggttcttc gtcaacgtcg ctttctatct ggtccaccaa
     1561 ctgcggaacc cgctcttcgc cggcgtccat gacgagacct ctatcgagat cgcgatgttc
     1621 atgccgtccg aatccaccca tgcaagccct gcttattgaa tcgtcccaaa cggattatgg
     1681 tcgacttctt cttcaacggt cgtggcaggt ccatgcccgg gaaagagacg atagtcgggt
     1741 gaaagggtca gcacacggcg gcgaactgaa ttgagatggg tcgaatacaa ttccttggga
     1801 ttggaccggc cgatcgatcc ggcaaagaga gtatcaccga cgaagcagac cgggtgttgt
     1861 gcctcatcca gccggtagca gataccgccc ggtgtatgac ccggcgtcgt cacacagtga
     1921 accgtacggc ggccgacact gatcgacatt ccatcgctcg gcgtaaccag taaatccgtc
     1981 cgtggtcgcc aactcaacag actgatatct tccggcccca ggtacaccgg gatttcgtgc
     2041 gagtctaaaa tctgctcgat cccctccgca tggtccgcgt gaccgtgcgt gagacaaatg
     2101 ccgacaaggc gcattcctcg tcgtcgaagc agatcaagca tcgcaggggc attataggcg
     2161 gtatcaacta acaatgcctc accttcatca tgcacgacat atccctgcac gccatatcca
     2221 ttaatggacc catgaaccat gtccacccag ggcggcatgc gttgggcaac cggctcccac
     2281 ttatcaatgg cgatttgctc gatcggctca gcgcgcagac ccaaggcctt tgccaaggca
     2341 cgcacctcgg catggtctcg tggctgatcg ccacgttcca gcgcagtaat atcgccgccg
     2401 ggcaatcccg tcatccttgc aacatcgccg acggacaatc cttgtccggt gcgtgctttt
     2461 ttaagaatgt cggataaatc atcttcgagc gacatgcgcg agccttctcc gatcagtgac
     2521 cacggaggtt tgccgaaact agcaaaccct tca
//
LOCUS       DATMTI010001099         1879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_168773, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001099 DATMTI010000000
VERSION     DATMTI010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168773"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VN039_17345"
     CDS             complement(<1..435)
                     /locus_tag="VN039_17345"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HWV47765.1"
                     /translation="MQILIGDILKSKAQTLINTVNCVGVMGKGIALEFKNRFPDMYED
                     YVQRCQRREVKPGIPYLYKTLFPPQIINFPTKDHWKSISKVSDIERGLKHLVEYYKEW
                     GVTSLAIPPLGCGNGQLEWRVVGPLIYRFVKDIGIPVEVYAPY"
     gene            complement(445..1035)
                     /locus_tag="VN039_17350"
     CDS             complement(445..1035)
                     /locus_tag="VN039_17350"
                     /inference="COORDINATES: protein motif:HMM:NF025840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HWV47766.1"
                     /translation="MNRSDIRELHYIAPIVNVPSILEHGLLSHHKAAKLSHRSVAMEE
                     IQDRRRVKRIPNAGPLHDYVNLYFDAHNPMLSRCRRHNDSICILRVKATVLELPEVIV
                     TDRNAAAEWARFYRVAEGLRALEKELVFAEYWTNSTDLLDERRRKAVKCAEVLVPSSI
                     APELLLGAYVANERALRTYQDLDIPLSVVIKSGMFF"
     gene            complement(1199..1543)
                     /locus_tag="VN039_17355"
     CDS             complement(1199..1543)
                     /locus_tag="VN039_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HWV47767.1"
                     /translation="MRKDDDVRLRHMLDAAREALGFVRGRNRVDLNSDRQLVLALVKA
                     IEIIGEAAFRTSENTRAQFPEIRWEDIVGMCHRLVHAYFDINLDVLWKTVQDDLAPLI
                     ACLEQVLDTKRH"
     gene            complement(1530..1655)
                     /locus_tag="VN039_17360"
     CDS             complement(1530..1655)
                     /locus_tag="VN039_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47768.1"
                     /translation="MERELSTLFGGRKVDLRTPEDLSRYFREDVLKEAEVQYAQG"
     gene            1654..>1879
                     /locus_tag="VN039_17365"
     CDS             1654..>1879
                     /locus_tag="VN039_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47769.1"
                     /translation="MRAIAEQARNVGRFELYKYINVAVGAKIGPQHGSEKGHAADMSL
                     PAKFRKLLLVNRYVRHQSTPPTSRATLSTAG"
BASE COUNT          436 a          521 c          486 g          436 t
ORIGIN      
        1 ataaggagca tatacttcaa cgggaatacc aatgtccttt acgaaccgat agatcaaggg
       61 gcccaccacg cgccactcca attgcccatt gccgcacccg agcgggggta tcgctaagga
      121 ggtgacaccc cattctttgt agtactctac taggtgcttt aacccgcgct caatgtcact
      181 gactttggag attgacttcc agtggtcttt cgtcgggaaa ttgatgatct gcggtgggaa
      241 caatgtcttg taaagataag gaatgccggg cttcacttcg cgacgttggc atcgttggac
      301 gtaatcctca tacatgtccg ggaatcgatt cttgaattcc agcgcaatgc cctttcccat
      361 gacccccacg caattgaccg tgttgatcaa ggtttgcgcc ttcgatttga gtatgtcgcc
      421 aataaggatc tgcattggtc tcgactagaa gaacatacca ctcttgatta caacggataa
      481 cggaatgtct aaatcctgat aagttcgcag ggctcgctca ttcgccacgt aggccccaag
      541 cagcaactcg ggagcgattg aagacggaac cagcacttct gcgcatttaa ctgctttgcg
      601 acggcgttcg tcaaggaggt ccgtagagtt cgtccaatac tcggcaaata cgagctcctt
      661 ttcaagcgcg cggaggcctt ctgccacacg gtaaaatcgg gcccactccg cagctgcatt
      721 cctatctgtg acaatgacct cgggcagttc gagaacagtc gcctttactc tcagaataca
      781 gatggaatcg ttatgtcgac ggcacctact gagcatcggg ttatgggcat cgaagtacag
      841 gttcacgtag tcatgcagcg gaccagcatt tggaatgcgc ttcacacgcc gtctgtcttg
      901 gatctcttcc atggctaccg accgatgcga tagtttggca gccttgtggt gtgacaggag
      961 accatgttcc aagatcgatg ggacattgac gatcggcgcg atgtaatgga gttccctgat
     1021 atcagaccgg ttcacttatg gtcctcgggt atatcacgct aagtcggtca ggagacctcg
     1081 aagactttca gcacttcgtc gccgtcgcct agcctccgat gcacaaaaca agatgtgatc
     1141 ccatcgggcg accataggca gataaacgcc ttcagtgcta gcttctttcc ggggaccgtc
     1201 aatgcctttt cgtgtccaac acctgctcga ggcaagcgat gaggggtgct aggtcatcct
     1261 gtaccgtctt ccagaggaca tccagattga tgtcgaaata ggcgtggacg aggcgatggc
     1321 acatgccgac aatgtcctcc cagcgaatct ctggaaattg agcccgcgtg ttttcggaag
     1381 tgcgaaaagc tgcttcaccg atgatctcaa tggccttgac cagcgccagc accaactgac
     1441 ggtcgctgtt aagatcgacg cgatttcggc ctcgcacaaa tccgagcgct tcacgcgccg
     1501 catcgagcat gtgccgcagc cggacgtcat catccttgcg catattgaac ctcggcctcc
     1561 ttcaacacat cctcgcggaa atatcggctc aaatcctcgg gcgtgcgaag atccactttg
     1621 cggccgccga acagtgtgga caattcacgt tccatgcgcg cgattgccga acaggccagg
     1681 aacgtgggca ggttcgaact ctacaagtac atcaatgtcg ctgtgggggc caaaatcggc
     1741 ccgcagcacg gatccgaaaa aggccatgcg gcggatatgt cgttgccggc aaaattccgc
     1801 aagttgttgt tggtcaaccg atatgttcgc catcagtcta ctccccctac ctctcgggct
     1861 actttaagca cagcggggg
//
LOCUS       DATMTI010001100         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_212046, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001100 DATMTI010000000
VERSION     DATMTI010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212046"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VN039_17370"
     CDS             complement(<1..182)
                     /locus_tag="VN039_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47770.1"
                     /translation="MIQTLFQEKLQVLNVGLSSFADSITKTGGSVLQIEWAPPAQGQQ
                     EVGRVLARLVNLRSVET"
     gene            complement(179..1735)
                     /gene="fdrA"
                     /locus_tag="VN039_17375"
     CDS             complement(179..1735)
                     /gene="fdrA"
                     /locus_tag="VN039_17375"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HWV47771.1"
                     /translation="MARATKIIRNFYRDSVSLMQLSSTFDKLSGVERASVVMATANNL
                     SLLQEAGLLTEPVEASANDLLIALQGEAAALESALATAESTLKQPSPSSTGNEKSRGI
                     SPRSIEMGLGNLVGANLALISTPGEYAAAEAFKALSLGLNVMLFSDNVDLKDEIALKR
                     FAQERDLIVMGPDCGTAIVNGIPLAFANVVRRGVIGVVGASGTGLQQVTCLVDRWGSG
                     ISQALGTGGHDLHREVGGISMLQGLKALAADPSTSVIVLISKPPSPEVADKVLEAAGR
                     AGKAVVVNFLGADPDRVRHENIYPAVTLEDAAAAAVALANGRTPEGRRASVTSPSITP
                     FAPQQRYIRGLYSGGTFCYEASLLLKKKLGQINSNTPVELVDRLSDVWTSQMHTVVDL
                     GDDRFTRGRPHPMIDHRLRNERLIMEASDPEVAVILLDVVLGYGSHPDPAAEMAPVIQ
                     KARDLATKAGRHLVIVGFVCGTAADPQNLSRQETVLREVGVTLAESNAQAVHMASSVV
                     LEAGIVGGRL"
     gene            complement(1993..>2259)
                     /locus_tag="VN039_17380"
     CDS             complement(1993..>2259)
                     /locus_tag="VN039_17380"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HWV47772.1"
                     /translation="ISGAYLRSAAKGHVSEPIAKLAQQLNQADTMSSVRAVTQTALQV
                     GHTSGSDGVLGMLLGCLAWQSLPPHLIFSDLLRLPVLRNSTAQS"
BASE COUNT          480 a          658 c          665 g          456 t
ORIGIN      
        1 gtttccacag agcgcaggtt gaccaaacga gccaacacac gtccgacctc ctgctgcccc
       61 tgagcgggcg gagcccattc aatctggagt acagagccgc ctgtcttcgt gattgaatcg
      121 gcaaaagaag acaggccgac attcaagact tggagcttct cctgaaacag agtctgtatc
      181 acaatcgtcc cccgacgatg ccggcttcca ggaccacaga ggaagccatg tgcacagcct
      241 gagcgttgct ctcggcaagg gtcactccga cctctcgaag aacggtttcc tgcctggata
      301 gattctgagg atccgctgct gtgccgcaga cgaaccccac gatgaccagg tgtctgcctg
      361 ctttggtggc gagatcgcgc gccttctgaa tcaccggggc catctcagcg gcaggatccg
      421 ggtgagatcc gtagccaagg acaacgtcca acaggatcac cgccacctcg ggatcgcttg
      481 cttccatgat cagccgctca tttcggagcc ggtggtcgat catgggatgg gggcgaccac
      541 gagtgaagcg atcgtcgccc aaatcaacga ccgtatgcat ctgactggtc cagacatcgc
      601 tcaaccgatc cactagttcc accggagtgt tggaattgat ttgccctagc tttttcttca
      661 gaagaagcga cgcctcatag caaaaggtgc cgccgctata gaggccccga atgtatcgct
      721 gctgcggagc aaaaggcgtg atggaaggag aagtcacgct cgccctcctg ccttcgggag
      781 ttctgccgtt ggcgagcgcc accgctgccg ccgcagcatc ctcgagcgtc acggcggggt
      841 aaatattctc gtgtctcact cggtccggat cggcgccaag aaaattgacg accaccgcct
      901 tgccagcgcg gccggcggcc tccagtactt tgtcggctac ttccggcgac ggaggtttgg
      961 aaatgagcac aatcacggaa gtggagggat cagctgccag cgccttaagg ccttgaagca
     1021 tggaaatacc gccgacctcg cggtgcagat cgtgtccgcc cgtaccgagc gcctgcgaga
     1081 tgccgcttcc ccatcggtcg accaggcaag tgacctgttg gagaccggtg ccggatgcgc
     1141 cgacgactcc gatgactccg cgccgcacca cattagcgaa cgcaagcggg atgccgttga
     1201 caatggcagt tccgcagtcc gggcccatga caatgagatc acgctcctgg gcgaaccgtt
     1261 tgagcgcgat ctcgtccttg agatcgacgt tgtcgctaaa gagcatgaca ttgaggccaa
     1321 gactcaatgc cttgaaggcc tccgccgcag cgtactcacc cggagtggag atcaaggcga
     1381 ggtttgcgcc gacgaggttc ccgagcccca tctcgatgct gcggggagaa atgccgcgtg
     1441 atttttcatt gccggttgac gaaggggaag gttgcttcag tgtggattct gccgtggcga
     1501 gcgccgattc cagggcagca gcttctcctt gaagagcaat caagagatcg ttggcggagg
     1561 cttcaaccgg ttctgtcagc aagccggcct cttggaggag gctgaggtta ttggcggtgg
     1621 ccatcacaac cgacgctcgt tccacgccgg acaacttgtc gaacgtggag gaaagctgca
     1681 tcaacgagac ggaatcacgg tagaaattcc ggattatttt tgtcgccctg gccatgtcta
     1741 cggcctcccg ttgatgccgt tgcctggtcg gcgcggtctt caacgtcagg cgggtggatc
     1801 ggcctcccgc cgatggcttg tgtggcgaac gtcaaatccc gtaacgtgat gcctcatgtc
     1861 tgatccgggc cgttaaattg aacacaacat tgtataccaa atcaagggca aaaccggggt
     1921 tcgagtcata ggcagaatca gatcgaggaa cggacatcgt ctcatctccg cttgcatctt
     1981 tcgattccgg tttcatgatt gggccgttga attgcggaga acaggcaaac gaagcaggtc
     2041 gctgaaaatc agatgaggag gcaggctctg ccaagcaagg cagcccagca acatccctaa
     2101 cacgccgtcg ctgcctgacg tatggccgac ttgtagagct gtttgcgtga ccgccctgac
     2161 gctgctcata gtgtccgcct ggttcagctg ctgcgccaat ttcgcgatcg gttcagagac
     2221 atggcccttg gctgcggatc ggagataagc gccgctgat
//
LOCUS       DATMTI010001101         1941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_635162, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001101 DATMTI010000000
VERSION     DATMTI010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635162"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VN039_17385"
     CDS             complement(<1..347)
                     /locus_tag="VN039_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47773.1"
                     /translation="MPATIQAEDGIGLRPLRTILPAKLASILSSDTHVLIERSAIEAF
                     LLALEGAPPDWATVYGHGHHDPGHDERLFHLNRERDIAREGNPVLNWRVAFVWPGELS
                     QFDPKTKSYAVAVG"
     gene            complement(397..723)
                     /locus_tag="VN039_17390"
     CDS             complement(397..723)
                     /locus_tag="VN039_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47774.1"
                     /translation="MGIPDEQAVQCAISAISQSDPLIKLLQQVRLGRMKPTDAGLRAV
                     TESWLGTYEKALATDGVTQSGLRRLNPAPRLAVLIEIGVLTNEHSGVTALRAAYDELL
                     ARASVE"
     gene            1027..1299
                     /locus_tag="VN039_17395"
     CDS             1027..1299
                     /locus_tag="VN039_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47775.1"
                     /translation="MYQEDKTFTIRITLEASFPDDYTGDEDERNWLHEWEARIKPQLL
                     KSVFESLRRHGGWASHIRNRGVSPADEIEIVVAKDFSQPLPFPLKR"
     gene            1296..>1941
                     /locus_tag="VN039_17400"
     CDS             1296..>1941
                     /locus_tag="VN039_17400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWV47776.1"
                     /translation="MSPSDEDLGLYVHIPFCRRRCHFCAFYLEIARSDRMGLFRSALA
                     QEIALYRRQDLLDGRVFQSIYFGGGTPTSLPADQLVALLTLIRATWPTAGAAEITVEA
                     HPSSITVRDLTILADAGFNRISFGAESMADKDFVSIGRFGHVQDTAAAVQAARQAGFR
                     NINLDLMYGLPGQSLQDWICILESLMTLAPSHISCYALTIEEGTRLAQDIARNCV"
BASE COUNT          424 a          576 c          539 g          402 t
ORIGIN      
        1 ccgaccgcca ccgcatagct cttggttttg ggatcgaatt gcgacaattc tccaggccag
       61 acgaacgcca cacgccagtt cagaacgggg ttcccctcgc gtgcaatatc ccgctcgcga
      121 ttcaggtgga atagccgctc atcatgtccc ggatcatggt gaccgtggcc atagactgtg
      181 gcccaatcgg gaggggcccc ttccaacgcc agaagaaacg cttcgatggc gctccgctcg
      241 atcaggacat gcgtgtcaga ggaaagaatc gaggcaagct tggccggaag aatcgttcga
      301 agagggcgaa gcccgattcc atcttccgcc tgaattgtag ccggcaagat gatcagaccg
      361 gacagccaca agaaagaaag gaggggtgcg cgcaacctat tcaacggagg cacgagcgag
      421 cagttcatcg taagcggccc tcagggcagt cacgcccgag tgctcgttgg tgagcactcc
      481 gatctcgata agaaccgcta accgaggggc gggattcagc cgccgaaggc cggattgagt
      541 cacaccatcc gtggccaagg ctttttcata ggtacccagc caagactccg tcacggcgcg
      601 caagccggca tcagttggtt tcatgcggcc caatcgcact tgctgcagca gcttgatcaa
      661 cggatcggat tgcgagatgg ctgagatcgc gcattgcacc gcttgttcgt ccgggatgcc
      721 cataggctat tcaattcatc gagcaagcgc ctggtccgcg aggatcaccg caactaccgc
      781 acgcgccggg accggctgag ggtgcccagt tgctcgtggc cccgactcca aaggcggaga
      841 actgtttatc cagcttggtc gtcttgcact ggggacaaac cgcctccgcc gatccttgga
      901 ccaacagctc aaaacggtga ttgcattcgc ggcacacata ttcgaagata ggcataggtg
      961 acgactcctt cggttaccga gagcattata aaattacgac gaccgaatcg caacgacagg
     1021 gatctcatgt accaggaaga caaaacgttt accattcgca tcacgctgga agcctcattt
     1081 ccggacgact ataccggaga tgaagacgaa cgcaattggc tgcacgagtg ggaagcccgg
     1141 atcaaaccgc aactgctcaa atccgtcttt gagtcgctcc gcaggcatgg cggatgggca
     1201 tcgcacatcc gcaatcgagg tgtatcgccg gccgacgaaa ttgaaatcgt cgtggctaag
     1261 gatttctcac aacctcttcc cttcccgctg aaacgatgag cccttcggac gaagacctcg
     1321 gtctgtatgt tcacatcccc ttttgtcgcc gccgctgcca cttttgcgcc ttttatctcg
     1381 aaatcgctcg atccgatcgc atgggtctgt ttcgctccgc cctcgcccaa gaaattgcgc
     1441 tctatcgtcg gcaagatctg ctggatggcc gcgtcttcca gagcatctat ttcggcggcg
     1501 ggacgccgac gtccctcccc gccgaccagt tggtcgcgct tctgacgctg attcgagcca
     1561 catggccgac cgccggcgcg gccgagatca cagtggaagc ccacccctct tccatcaccg
     1621 taagagatct cacaattttg gctgatgccg gattcaatcg gatcagcttt ggagcggagt
     1681 ccatggcgga caaggacttc gtctcgatcg gtcgttttgg acacgtgcag gatacggcgg
     1741 ccgcggtcca ggctgcgcgc caggctgggt tcagaaatat caacctcgac ctgatgtatg
     1801 ggctgccggg ccaatcgttg caggattgga tatgtatctt ggaatcgctc atgacgcttg
     1861 ctccatcaca tatctcttgt tatgccttga ccattgagga gggtaccagg ctcgctcagg
     1921 acatcgctcg gaattgtgtt c
//
LOCUS       DATMTI010001102         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_338897, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001102 DATMTI010000000
VERSION     DATMTI010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338897"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VN039_17405"
     CDS             <1..261
                     /locus_tag="VN039_17405"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HWV47777.1"
                     /translation="TALLAQRWFASQLSLSDAIILGILLTVTGLLGDLFESVIKRRTG
                     VKDSGGILPGHGGMLDRIDSLLFTAPTFYYYVAYIRGLSPPL"
     gene            282..1442
                     /locus_tag="VN039_17410"
     CDS             282..1442
                     /locus_tag="VN039_17410"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWV47778.1"
                     /translation="MKSIIILGSTGSIGTNTLDIVRRFPEEFRVVGLTAGNNIDKLEA
                     QIRQFNPAAVAVSTESSAALLRNRCADLPVEIMAGEEGITQAASALDAELVISAIVGA
                     AGLVPTLSAIRSGKHIALANKEPMVMAGKLMQDEARKHGVRIFPVDSEHSAIFQSLEG
                     HRIEDVRRLILTASGGALWTLPQEQLLNVTPERALQHPNWKMGSKITIDSATLMNKGL
                     EVVEARWLFNIPESNIDVLIHRESIIHSLVEYRDRSMIAQLGLPDMRTPISYAMRHPA
                     RMALDLPSLDLTEIGQLTFCKPDHDRFPCLNLGYESLRIGGTMPAAMNAANEIAVEAF
                     LNHGLRFSEIPQVIRSTMEAHTHQEITCLDDALEADRWAREKAESLVHALPR"
     gene            1509..>2431
                     /gene="rseP"
                     /locus_tag="VN039_17415"
     CDS             1509..>2431
                     /gene="rseP"
                     /locus_tag="VN039_17415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HWV47779.1"
                     /translation="MTSVLAWSPDTLWLLLQKAWWFLVVLGVLVAFHELGHFLAARWV
                     GVKVLKFSLGFGPKLFGRQVGETEYLVSAVPLGGYVKLFGEDETEATTPDDRRRSFSH
                     QGLWGKVLIVAAGPGFNFILAYLIFAGWLSTGAPLFVPTFRDLSADVEALVPGSPASM
                     AGMEVGDRIVKVNGKDISTKTELLDIVSKSKGQTVSLEVRRADQLKTLTATPIPVTGE
                     GANGDDPLYTIGVEETPPLVTSVMHGSPAALAGFKPGDRVVAIEGQAVYTWAQMTIQV
                     REHPQKPLKLDVLRDGKRTALTVTPTSEKVTA"
BASE COUNT          532 a          685 c          683 g          531 t
ORIGIN      
        1 accgcattat tggctcagcg gtggtttgcc tcacaactct cactgtcaga tgcaatcatt
       61 ctcggcatac tactaacagt gacaggcttg ctcggagacc tatttgaatc agtgatcaaa
      121 cgtcggacgg gcgtcaaaga ttccggcgga atcctgcccg gccatggcgg tatgttggac
      181 cggatcgata gtctcttgtt caccgccccc accttctact actatgtcgc ctacattcgc
      241 ggcctgtcgc cgcctctatg agaggattga gaggagagga tatgaagtcg ataatcatct
      301 tgggatcgac cggatccatc ggaaccaaca cgctcgatat cgtccgacgg tttcctgaag
      361 aattccgggt cgtcggactg acggccggca ataatatcga caagctcgaa gcgcagattc
      421 gccaattcaa ccccgcggcc gtggcggtct ccacggaatc ctctgcggct ttgctccgaa
      481 accgatgtgc cgatcttccg gtggagatca tggcagggga agaaggcatc acacaagcgg
      541 cgtcggcgct ggatgccgaa ttggtgattt ccgccatcgt gggagcggca ggactcgttc
      601 cgaccttgtc ggctatccgg agcggaaagc acattgccct cgccaataag gagccgatgg
      661 tgatggccgg taagctgatg caggacgagg cccggaaaca cggtgtcagg attttcccgg
      721 tggacagcga acacagtgcc atctttcagt cattggaggg acatcggatt gaagatgtcc
      781 ggcgattgat tcttacggcg tcaggcggag ccctttggac actgccccaa gaacagctct
      841 tgaatgtgac accggaacga gccctgcagc atcccaactg gaagatggga tccaaaatca
      901 ccatcgactc agccacgttg atgaacaaag gattggaagt tgtggaagcc cgctggctct
      961 tcaatattcc cgagtccaac atcgacgtct tgatccatcg agagagcatc attcactctc
     1021 tggtagagta tagagatcgg tccatgatcg cccaactggg acttccggat atgcgaaccc
     1081 ctatctccta tgcgatgcgt catcccgcca gaatggccct tgacctcccc tcactggatt
     1141 tgacggagat tggacaattg accttttgca aacccgatca tgatcgcttt ccctgtctca
     1201 atctcgggta cgagtcgctt cgaatcggcg gcacgatgcc tgctgcgatg aatgcggcga
     1261 acgaaatcgc cgtcgaggcg ttcctcaacc atggcctccg cttcagtgaa atcccgcagg
     1321 tcattcgcag tacgatggag gcgcataccc atcaggagat tacctgtctc gatgatgcgc
     1381 tggaagcgga tcgatgggct cgtgaaaagg ccgaatcatt ggtgcatgcg ttacctcgct
     1441 gacgattgtt tgcatttcaa gccgcgaatg ttaaagtaga agatcataca cgcgacacgg
     1501 agttcctgat gacgtccgta cttgcctggt cccctgatac gttgtggttg ctcctccaga
     1561 aggcctggtg gtttcttgta gttcttggcg tactggtcgc cttccatgag ctcggccatt
     1621 ttctcgccgc gcggtgggtc ggagtgaaag tcctcaagtt ttctctcggc ttcggcccga
     1681 agttattcgg ccgccaggtc ggcgaaaccg agtatctcgt ctctgccgtg ccgctcggcg
     1741 ggtacgtcaa gttgttcggt gaggatgaga cggaggcaac aacacccgac gaccgtcggc
     1801 gatcgttttc gcaccaaggc ctgtggggaa aggttcttat tgtggcggca ggccccgggt
     1861 tcaattttat tctggcctat ctgatcttcg ccggatggtt gtccactggt gcgccgttgt
     1921 ttgtcccgac gttccgcgat ctgagtgccg acgtcgaagc cctggttccc ggttctcccg
     1981 catcaatggc agggatggaa gtcggcgatc gcatcgtcaa ggtcaatggc aaagacatct
     2041 cgaccaaaac ggagttgctg gatatcgtct ccaagagcaa gggccagacg gtgtcactcg
     2101 aagtccgacg agccgaccag ctgaaaacac tgacggcaac tcctattccg gttaccggag
     2161 agggggccaa tggagacgac cccctataca cgatcggtgt tgaagaaacg cctcccctcg
     2221 tcacctcggt catgcatgga tcgcccgccg ctctggccgg attcaagccg ggcgatcgag
     2281 tggtcgcgat agaagggcaa gccgtctata catgggcgca aatgaccatc caggtaagag
     2341 agcaccccca gaaaccgctg aaactcgacg tcctccgaga cgggaaacgt accgcactga
     2401 ccgtcacgcc gaccagcgaa aaagtgacag c
//
LOCUS       DATMTI010001103         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_125485, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001103 DATMTI010000000
VERSION     DATMTI010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125485"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1558)
                     /locus_tag="VN039_17420"
     CDS             complement(200..1558)
                     /locus_tag="VN039_17420"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWV47780.1"
                     /translation="MADALEDLLDALEDVDDATREEAARMLADMGDPTTLDALVSACN
                     DEFWSVRAHAGCGVAKIGGPKAIEALIGLFNDPIMEVRDQAVEAAAKMGPIMLDRLVA
                     AVKDERWRVREHAAKAAGRIKDSRAVDALIGACRDRDGAVKSAAAEALGRIADPKAIP
                     ALIKLFHDSSKIVRETAGTALVYIGHASVDPLIDSLKDKDFVVRCHAARALGGMTTDY
                     QIGRSWVRDAKVVDALISALKDPDRAVREDATIALGMIGDSRAIDALVEAMKDGAVKR
                     HAIASLGMIGDSRALPAVLDALKGKGIKQEGSPTPGCIVSEDAFIKEAAATALGQFRN
                     PRVIPDLIMLLKDGVLREKAAAALAVIGDAAIEPLIAFLYDPKASEVEAEKERVLSYA
                     SVRLTAKDALKQIALDTLEKLGWTPPDETVEISSSQADNLRVDRPLGKTGRFGPSGDL
                     AN"
     gene            complement(1551..2021)
                     /locus_tag="VN039_17425"
     CDS             complement(1551..2021)
                     /locus_tag="VN039_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47781.1"
                     /translation="MRYAALIRRWSLPVFGWALTSLLAMVSPQDGFAGDPPAAHLREH
                     GITIDATKLVPTSWWQVPGVTPSIWNSDLDSLDAPKTSELRALQLRPGKYKFVSFTFD
                     FPFTVTLNGTLDFSKSLDQCVEGRGTRTLVVLCKRTYPYGGQRDAYYEQKPNDG"
     gene            complement(2024..3043)
                     /locus_tag="VN039_17430"
     CDS             complement(2024..3043)
                     /locus_tag="VN039_17430"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWV47782.1"
                     /translation="MTHPSRRLHTILVGLLTILLEIGAQAPVDAAPPTGKETAPSGSG
                     TEHVILFVLEGFGQKSLQGGTMPVLSKLVKEGSVTWSASGVKPALRLPTMASLVTGMP
                     VEKHGITWNFFEFSRGYPRAPSLFDYLDLSGGRDSAIFYMDESLYQLAKPEPYTDYQL
                     CGALRPECGSQKLVAYIQQYLQKATSGHGYGHAILSLPHLLVVHLPEAGRAGVTHGWD
                     SKEYREALRTVDGAMKSILGLFKKYSLLDRTAVVITALSEKGTDPGAEAPTTDSPVVP
                     WIVSGAGIKHGQLIRQPVSIIDTGATVMKILGLETHTEWESKVVEEIFQTAAATPAVQ
                     PLKKR"
     gene            3219..4238
                     /locus_tag="VN039_17435"
     CDS             3219..4238
                     /locus_tag="VN039_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47783.1"
                     /translation="MSGRLFHQPPFPLFLPSHKSYRPMAVFLALAAIMAPSPSLAEIR
                     VVNAQGEHRMGNRDTREDAIRLATEAAKRNALEQVATYLESVTVVHDMDVTKDEIRSY
                     TAGLVLVLNQQITTTLDGDVVVVKTDLLAQMDTDEVARAIAALRENEDARVQLAALKQ
                     ENEQLQEELNAVNQTLGNAATAEQVQQAAQKRKEILNRVQSNAMVSQAWTNWVLVSPV
                     GFPQALLNSASGLSPANPHIAIAQQIIANRQPLGPRQPPAPGNRQPQMPRHELIPPPG
                     MSGVPRTPNEIINRAPPGRIQSDHQPMTQPGQGSVRQQSRPHYRFDQSLGVPGGGPRS
                     GSGRP"
     gene            4235..4795
                     /locus_tag="VN039_17440"
     CDS             4235..4795
                     /locus_tag="VN039_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47784.1"
                     /translation="MNGSVKIAVVMGLYLIEGLIVTGVQAVDTEDVTTRVERSFDQLK
                     NYQEEKAADKSSSVRHTVEVGHGAYSSDQYWIGRGQGDLTKGKVVCQRVSELSARTDL
                     AKQIRVLVKEHMIDRVRERSGRESEQDIELTREEIVQEYLQDVKIVDRRIDEEQKICT
                     AIAVMPKSRLQSKPTDSLEPTSTVVR"
BASE COUNT         1035 a         1377 c         1410 g         1002 t
ORIGIN      
        1 ccgcacgcgg atctttgaag ttgccgagca ggccggccgc ttcaccacgg atcgcccaat
       61 cctcgtccct gagtgccgcg atctgctctg tgacggcatc ggccatcctg ttttcccttc
      121 ttgcaggatt cagcggttca cttcagaata aatgttttca tggcaactgg acggcagctg
      181 tgaacctggc tgcagggggt caattagcca agtcgccgga cgggccaaag cgtccggttt
      241 ttcctaacgg ccgatcgacg cgcagattgt cggcctggct tgagctgatc tccaccgttt
      301 catcaggagg cgtccatcca agtttttcca acgtatcgag ggcgatctgc ttcaaggcgt
      361 cttttgccgt cagtcgaacg gaggcgtacg aaagcacacg ttccttttca gcctcgacct
      421 cagacgcttt gggatcatag aggaaggcaa tcagtggttc gatggctgcg tctccgatca
      481 cggccagagc cgccgccgcc ttttcccgca agaccccgtc tttcagcaac atgattaaat
      541 ccgggatcac acgaggattg cgaaactgac cgagcgcagt ggcagcggct tccttgatga
      601 acgcatcttc gctgacgatg cacccgggtg tcggcgagcc ctcctgcttg atgcctttgc
      661 ccttcaaggc atccaatacg gcggggaggg cgcgcgaatc gccgatcatg ccgagcgagg
      721 caatggcatg gcgcttcacc gctccgtctt tcatggcttc taccagcgca tcgatcgccc
      781 gagagtcgcc gatcatgcct aatgcgatgg tcgcgtcttc acgaacggct cggtcagggt
      841 ccttcaaggc ggagatcaag gcgtctacga cctttgcgtc tcgtacccag gacctgccga
      901 tttgatagtc ggtggtcatc ccgcccaacg cacgagccgc atgacatcga accacaaagt
      961 ctttgtcctt gaggctgtcg atcaacggat cgaccgaggc atgcccgatg tagaccagcg
     1021 ccgttccggc tgtctcgcgc acgatcttag aagaatcatg aaacagcttg atcagggccg
     1081 gaatagcctt cggatcggca attctgccca aggcttccgc cgccgcgctc ttgacggccc
     1141 cgtctcggtc gcgacaggcg ccgatgagcg cgtcgacggc tcttgagtcc ttgatcctgc
     1201 cggcggcctt ggcggcgtgt tcgcgtacac gccaacgttc gtctttcacg gcagccacca
     1261 ggcgatcgag catgatcggg cccatcttcg ccgcagcctc gaccgcctga tcgcgaactt
     1321 ccatgatggg atcgttgaac aggccgatca gggcttcgat cgccttcggc ccgcctattt
     1381 ttgcgacgcc gcagccggca tgggcccgga cggaccagaa ctcgtcgttg caggcgctga
     1441 ccagagcatc caaggtggtc ggatcaccca tgtcggcaag catccttgcc gcctcctcac
     1501 gggtggcgtc gtccacatct tcaagcgcgt ctaaaagatc ttcgagtgcg tcagccatcg
     1561 ttcggtttct gctcgtaata ggcatcgcgc tgtccgccgt agggatacgt gcgtttgcac
     1621 aacaccacca aggtccgtgt tccgcggccc tcaacgcatt gatccagtga tttggaaaaa
     1681 tcgagggtgc cgttcagagt gaccgtaaag ggaaaatcga aggtgaaact gacaaatttg
     1741 tacttgcccg gccgtaactg caacgcgcga agctccgatg tcttaggggc atcgagcgag
     1801 tcgaggtcgg aattccagat cgagggcgtc acgccaggta cttgccacca ggaagtagga
     1861 accagttttg tcgcatcgat ggtaatcccg tgttctctaa gatgggcggc cggcgggtct
     1921 cccgcgaatc catcctgagg gctgaccata gccagcaggc tggtcagcgc ccagccgaat
     1981 acgggaagag accagcgtcg tatgagggca gcgtagcgca taattagcgt ttcttgaggg
     2041 gctgtacggc cggggtggcc gcggcagttt ggaagatttc ttctacaacc ttactctccc
     2101 attccgtgtg ggtctccagc cccaggatct tcatcacggt ggctccggta tcgatgatag
     2161 acaccggctg acgaatgagt tggccatgtt ttattccagc tcccgagacg atccagggaa
     2221 cgaccgggga atcagtcgtt ggagcctcgg cgccggggtc cgtccctttt tcgctgagag
     2281 cggtgatgac gacggcggtg cggtctaaga gcgagtattt cttgaatagg cccaggatag
     2341 acttcatcgc gccatcgact gttcgtaatg cctcgcgata ctctttagaa tcccagccgt
     2401 gggtcacgcc ggcccttccg gcttccggaa ggtgaaccac cagcaaatgc ggcagggaga
     2461 gaatcgcgtg cccatatccg tgtccgctgg tcgccttttg aagatactgc tggatgtagg
     2521 cgacgagttt ctgcgagccg cactcaggcc gcaatgctcc gcagagttga tagtcggtgt
     2581 agggttccgg cttggcgagc tgatacaggg actcatccat ataaaagatg gcgctgtcgc
     2641 ggccaccgct tagatccaga tagtcgaata gactgggagc gcgaggatag cctcggctga
     2701 attcaaagaa attccaggta attccatgtt tctccaccgg catgccggtc accagcgatg
     2761 ccatggtggg caatcgcagg gccggtttga ccccgctcgc ggaccaggtc accgatcctt
     2821 ccttgacgag cttgctcagc accggcatcg taccgccctg aagcgacttt tgaccgaagc
     2881 cctccaggac aaagaggatg acatgttcag tcccagagcc ggagggagcg gtctccttcc
     2941 ccgtcggggg agctgcatcg acaggcgcct gggccccgat ttcaagcaag atggtcagaa
     3001 gaccgacaag gatcgtgtga aggcgtcgtg atggatgtgt catgacaaaa tatcacccgc
     3061 atgtatccta cgagtaagag tggtacccta acatgcaaat tttccggaag gcaaggtggg
     3121 ggaaggcatc cgccgattgc tggcggaaca accttccagg tgctatgctg ggaccgctga
     3181 ccgtttccat ccgagcgcag gggtgagaag gggaagccat gtccggtcga ttattccatc
     3241 aaccaccttt ccccttgttc ctgccgtctc ataagtcgta tcgacccatg gccgtttttc
     3301 tcgcactcgc ggcgatcatg gccccctctc cttcgcttgc tgaaatcagg gtcgtcaatg
     3361 cccagggcga acatcgcatg gggaaccgcg acacccggga agacgcgatc agactcgcta
     3421 ccgaggcggc caaacggaat gcactcgaac aagttgccac ctatctcgag agcgtcacgg
     3481 tcgttcatga catggatgtc accaaggacg aaatccgatc ctatacggcc gggttggtgc
     3541 ttgtgctcaa tcaacagatc accacgacat tggacggtga tgtcgtggtc gtgaagacgg
     3601 atcttctcgc gcagatggat acggacgaag tggcgcgggc gatcgcggcg cttcgcgaaa
     3661 acgaagatgc ccgtgttcag ctggcggcgc tgaaacaaga aaatgaacag ctgcaagagg
     3721 aactcaatgc cgtcaatcag actctgggca atgcagcaac agcggagcag gttcaacagg
     3781 cggcccaaaa gcggaaggaa attctcaatc gtgtgcaatc gaacgcaatg gtgtcgcaag
     3841 cctggacgaa ttgggtgctt gtctcaccgg tcgggtttcc ccaggcattg ctgaatagtg
     3901 cgagtgggct ttctccggcc aacccgcaca tcgcaattgc gcagcagatc atcgccaacc
     3961 ggcagccgct ggggcctagg cagccgcccg cgcctggaaa tagacagcct cagatgccac
     4021 ggcatgagct tattccacct cctgggatgt cgggtgtccc acggacaccg aatgagatca
     4081 ttaatagagc acctccagga cggattcaga gcgatcatca gcccatgacc caaccgggcc
     4141 aaggttctgt tcggcagcag tcacgcccac actatcggtt cgaccaatcc ttgggcgttc
     4201 cggggggagg tcctagatcc ggaagtggaa ggccatgaat ggctcggtta agatcgccgt
     4261 cgtgatgggt ctctacctta tcgaggggct gattgtcacc ggtgtgcagg cggttgacac
     4321 agaggatgtc acaacacggg tcgagcgatc gttcgatcag ctcaagaact accaggaaga
     4381 gaaagcagcg gacaagtctt catcggtcag acatacggtt gaagtcggcc atggcgcata
     4441 ttcttcagac cagtattgga tcggcagagg tcagggagat ttaaccaaag gcaaggtcgt
     4501 ttgtcagcgc gtgtcggagc tctcggctcg gaccgatctt gccaagcaga ttcgcgtatt
     4561 ggtcaaagaa cacatgatcg atcgagttcg tgaacggtcg gggcgcgaga gcgagcagga
     4621 tatcgagctg actcgtgaag agatcgttca ggaatatttg caagatgtga agatcgtcga
     4681 ccgccggatc gatgaggagc agaagatctg tacggctatc gctgtgatgc cgaagagtcg
     4741 gctacaatcc aaaccaacag atagccttga gcccacctcg acagtcgttc gctaaataat
     4801 tctcgttcat gcaacgggct atga
//
LOCUS       DATMTI010001104         7873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_592769, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001104 DATMTI010000000
VERSION     DATMTI010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592769"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1087)
                     /locus_tag="VN039_17445"
     CDS             complement(92..1087)
                     /locus_tag="VN039_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWV47785.1"
                     /translation="MTKVLVTGAAGFLGSSLVEELCRSGYQVRAVVHDSRLVTSCSRE
                     VEIVVADICDFKKIREITAGCGAIVHLAAKVHAIDDSGAEKDYEAVNVEGTRHILDAA
                     VASGVGHVVFASSVKVFGEETKGCIDESQTPDPQTPYGRSKWKAEQLVSEYAGRHGLI
                     AVSLRLPMVYGPTKKGNLYRMIEAIDHGRFPILPRLSAVRSLLHVQNFIQAVLLCLRA
                     PCFKQGAYIVADTEPYCVTDLYDWLRAGLGNAPPRWRVPLWILKGGARCGDMLQAING
                     RPGPLTTEQLAKLIGCAWYSSAAITRELDYRPMYSLEKAVPELIAFYRDEARAGC"
     gene            complement(1084..2244)
                     /locus_tag="VN039_17450"
     CDS             complement(1084..2244)
                     /locus_tag="VN039_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWV47786.1"
                     /translation="MSDRPRLLYLITEDWYFWSHRVDLARAAREAGYDVIVATRVTDH
                     GARIRGEGFQLEPLELVRRNRNPLREAIAVAELVRLYRRVRPDIVHHVAMKPILYGSL
                     VAWFTRVPAVINAFAGLGYTFMDDRNRLLRWCVKTALRTVLSLGHSVVLVQNHDDQDR
                     LVGEGVVPVSRTRIIAGSGIDIAVYSLQLQPSGVPIVVLPARMLWDKGVGEFVEAARR
                     LKRKGAHARFVLVGRRDEHNPAAIPEIRLKEWVKEGVVEWWGHREDMPAVYAAAMLVV
                     LPSYREGLPKVLLEAAACGKAMVATDVPGCREIVRDRFNGLLVSPRDSMALANAMEGL
                     LSDQESREIMGQRSRTLALSEWSGSRISEQVLTLYHDVLKSSSIADRSHGYA"
     gene            complement(2207..4174)
                     /gene="asnB"
                     /locus_tag="VN039_17455"
     CDS             complement(2207..4174)
                     /gene="asnB"
                     /locus_tag="VN039_17455"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWV47787.1"
                     /translation="MCGIAGMMFCRDAELSPIVSKMVEMIRYRGPDDSGLWCDADHGL
                     ALGHARLSILDLSPAGHQPMESASGRYVIVFNGEIYNHLELRGQLTGLPWRGHSDTET
                     LLTAFETWGVEKALQATVGMFALALWDRIERRLVLARDRIGEKPLYYGWSNGTFIFAS
                     ELKALEAYPGWRGEIDRDALALFMRYAYVPLPFSIYTGIRKLLPGTYVTIPSECPARY
                     WSEPKPYWSAAAVAGQAIESEWTDMAAEDELHRLLSRAIKGQMLSDVSLGALLSGGID
                     SSTVVALMQAQSSRPVKTFSIGFHDDEYNEAPQAKAVATHLGTEHTELYVRSADALAV
                     IPHLPTMYDEPFGDSSAIPTHLVARLAKQQITVALSGDGGDELFGGYNRYSWGRSIWH
                     RIARVPIPMRAMTAAALTMLSPEQWDRIGRILRPGLPASLHMSALGEKVHKLAAVLDS
                     DSQTELYRRLVSLQRETSSLVIGAQDKPIWADAQAQQLGGRNFSEEMMLHDLVGYLTD
                     DILAKVDRAAMAISLETRMPLLDHRVLEFSWSLPLSMKIREESQGKWLLRQVLYRYVP
                     KDLVERPKMGFGIPLDSWLRGGLRDWAEALLEESRLKREGYFDPAPIRAKWREHLSGR
                     RNRQYWLWNVLMFQAWLSAHERSPSSTVSYH"
     gene            complement(4199..5086)
                     /locus_tag="VN039_17460"
     CDS             complement(4199..5086)
                     /locus_tag="VN039_17460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWV47788.1"
                     /translation="MNAPLVTVAMSAYNASATIDLALRSILTQTYQNWELIVVDDGST
                     DSTREIVSFHAQDPRIRFIQEPSGNRGLAARLNQCVRLARGQYVARMDADDVAYPHRL
                     ERQVQFLEEHRDIDLLGTGAVIFKGEGEIIGCYPTAGSHEAICRRPWWGFPLAHPTWM
                     GKRAWFLSHPYSDEDTRCEDQALLLQSFSHSRFAALEEVLLGYRVAEIAAARLGRGRL
                     NYCRRLVERVRDLSSLRWAMMGFGVHSAALARDLALQLGRTVGSGSRRSWGSAGQAEK
                     DQWQSVWAGLARDHTVLHV"
     gene            complement(5262..6464)
                     /locus_tag="VN039_17465"
     CDS             complement(5262..6464)
                     /locus_tag="VN039_17465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV47789.1"
                     /translation="MKILCVLGEHAYGDPARGEGPEYANFIPALRRLGHEVTFFESLS
                     RKPYEDFSRLNHALLKRVEETSPDVVFCVLMHYEVWIETIQVIRNSGAWVVNWATDDS
                     WKYPMFSKLIGPKFDLFVTTYPQMGERYHHDGINSVCLSQWAANGDTLISSLPASACR
                     YPVSFVGAAYGNRRAMIARLRHEGIEVVCFGHGWPEGPIETKRMGEIFRESQISLNFS
                     DGAHGRSDFASRQIKARIFEVPGYGGCLLTEKAPDLDRYFHVGEEILAFEGADEMIQQ
                     VKSLLSRPERRDEVARCGFERVRRDHTYERRFEELLRELPQRVPRRPRIPIKWDGFEA
                     AVRDYRTSPVLRMIRFLLVFVASLVWGKKRGPRAARRLVFELSWRLRGAWTYSAAGWP
                     GRMFYKES"
     gene            complement(6677..7429)
                     /locus_tag="VN039_17470"
     CDS             complement(6677..7429)
                     /locus_tag="VN039_17470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV47790.1"
                     /translation="MSSGLRAWLAQSELLAYNAVRRDRWIAQQAALVPSGTKVLDVGA
                     GSCPYRALFAHCDYRTQDLAPLRGDQLRYGGYGALDFVSDVTAIPVPDGSFGMVLCTE
                     VLEHHPEPIRVVHELARILQPGGRLVLTAPLGSGIHQEPYHYYGGYTPWWYERFLKAA
                     GFEEVHIEPNEGSFRFFGQESIRFIQTTSPWSTSLSVGVRLLWFPMWIVLFPILGLIV
                     PIFCKWLDRFDRERRFTVGYHVTARRASSHYS"
     gene            complement(7552..>7873)
                     /locus_tag="VN039_17475"
     CDS             complement(7552..>7873)
                     /locus_tag="VN039_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47791.1"
                     /translation="RILHGNSASMLPLALASIKQSTLFWLDAHYSGGITACGDQHSPI
                     LLELEHIFRHPIRGHVIVIDDARLFGADSAYPTLEQLQLFIDAQSTPRKLEIIDDAIR
                     IYEV"
BASE COUNT         1880 a         2399 c         2028 g         1566 t
ORIGIN      
        1 attgaaatca agacgtccga gccttcgaac ccctatggct ggaagtgtta cgtctacgtg
       61 agccacatga gagctcaacc tatgagatgt ctcaacagcc cgcacgtgcc tcatcgcgat
      121 aaaatgcgat cagctcgggc accgccttct ctaaagaata cataggccga taatctaatt
      181 cgcgtgtgat agcggcagaa ctgtaccagg cgcagccgat aagctttgcc agctgctccg
      241 ttgtcaaggg accaggcctt ccattgatag cttggagcat atcaccgcat cgcgcaccgc
      301 ctttgagtat ccagagggga actcgccatc gcggaggtgc gtttcccaat cccgctcgta
      361 accagtcgta gagatcggtc acgcaatacg gctcggtatc ggctacgatg tatgcgccct
      421 gcttaaagca gggcgcgcga agacacagga gtactgcctg tataaaattt tggacatgta
      481 agaggcttcg gaccgccgac agacgaggca agatcggaaa cctcccatga tcgatcgcct
      541 cgatcatgcg atacaggttg cctttcttcg tcggaccata gaccatcgga agacgaagtg
      601 agacagcaat gaggccatgt cggcctgcat attccgagac aagctgttcg gccttccatt
      661 ttgatcgacc atagggggtc tgtggatccg gagtctgcga ttcatcgata caccctttcg
      721 tttcttcccc aaagaccttg accgaactcg caaacaccac atggcctacg ccggatgcta
      781 ccgctgcatc gagaatgtgc ctcgtgcctt cgacgttgac ggcttcatag tccttctctg
      841 ctccggaatc atcgatggca tggaccttcg ccgccagatg cacgatggct ccacagccgg
      901 ccgttatctc ccggatcttc ttgaagtcac agatatccgc gacaacgatt tctacttctc
      961 tcgaacacga ggtgaccaac cttgaatcat gaacgacggc tcgaacttga taccctgacc
     1021 gacagagttc ttcaaccagg gacgacccaa gaaaccccgc tgctcctgtt accagcactt
     1081 tggtcatgcg tacccatgcg agcgatccgc tatagaagaa gatttcaata catcgtggta
     1141 cagggtgagg acttgttcac tgatccttga accagaccat tcactaagag cgagtgttcg
     1201 gcttcgctgt cccataattt cccgcgactc ctgatcggac aacagtcctt ccatggcgtt
     1261 cgccaacgcc atggaatctc taggagaaac caacaacccg ttaaatcgat cacgcacaat
     1321 ctctcggcag ccgggaacat ccgtggccac catggccttg ccgcaggccg ccgcctccag
     1381 caacactttg ggaagcccct cgcgatacga gggcaacacg acgagcatgg ccgctgcata
     1441 gacagccggc atatcctctc gatgtcccca ccactcgacc accccttcct tcacccattc
     1501 cttcaggcgg atctccggga tagccgcggg gttatgttca tcccgtcgcc cgacgagaac
     1561 gaaccgggcg tgagcgcctt ttcgtttcaa ccgccgggcg gcctcgacaa attcaccgac
     1621 gcctttgtcc cagagcatgc gtgcaggcag aacgacaatc gggacaccgg acggctgcag
     1681 ctgtaaagaa tacaccgcga tatcgatgcc ggaacccgcg ataattctcg tacgagagac
     1741 aggaacgacc ccctctccga cgagccggtc ctgatcatcg tgattttgga ccagaacgac
     1801 cgaatgaccc aaactgagga cggttcttaa cgcagtcttc acgcaccagc gcaacaaccg
     1861 gttccggtcg tccatgaagg tatacccaag accggcgaat gcgttgatga ccgccggcac
     1921 tcgggtaaac cacgcgacca gagatccgta cagaatcggt ttcatggcca catggtgaac
     1981 gatatcgggt ctgacacgcc gatacaatcg caccagttcg gcgacggcga tcgcctcgcg
     2041 gagcggattg cggttccgac ggaccagctc cagcggctcg agctgaaatc cttctcctcg
     2101 gattcgcgct ccgtgatcgg tcacacgagt cgccacgatc acatcatagc ccgcttcccg
     2161 tgcggcccgt gccagatcga cacggtggga ccagaaatac caatcttcag tgataagata
     2221 cagtagacga gggcgatcgc tcatgagcac tcaaccaagc ttgaaacatc aagacgttcc
     2281 agagccaata ctgccgatta cgccgacccg agagatgttc ccgccatttc gctcggatcg
     2341 gagccggatc gaaatatcct tcacgcttga gtcgagactc ctccaacaac gcctcggccc
     2401 agtccctcaa gccgcctcta agccaggagt ccaacggaat gccgaaaccc atcttgggtc
     2461 gttcaaccag atctttcggg acatagcgat acagaacttg ccgaagcagc catttgccct
     2521 ggctttcttc cctgatcttc atcgaaagag gaagactcca ggaaaactcc aaaacgcgat
     2581 ggtccagcag gggcatcctg gtttccagac tgatcgccat tgcggcccga tcgactttgg
     2641 ccaagatatc atcggtcaag tatccgacca aatcgtggag catcatctct tcgctgaaat
     2701 tccgcccccc cagttgttgc gcttgagcgt cggcccaaat cggtttgtcc tgagcgccga
     2761 tgaccaaaga agaagtctct cgttgtagcg acacaagtcg ccgatacaat tccgtctgac
     2821 tatccgaatc caggaccgcg gcaagtttat gcaccttctc cccgagggcc gacatatgga
     2881 gcgaagcagg aagccccggc ctcagtatcc tcccgatcct atcccactgc tcgggcgaca
     2941 gcatggtcaa ggcagctgcc gtcatcgccc tcatgggaat tggtactcgc gcgattcgat
     3001 gccagatcga cctcccccaa gaataccgat tgtagccccc gaataactca tcgcccccat
     3061 caccggaaag cgcaaccgtg atctgttgct tggccaatct cgcgacgaga tgggtaggaa
     3121 tggcggatga atcgccgaat ggttcatcat acatcgtcgg cagatgcgga atcaccgcca
     3181 aggcgtcggc cgatctcaca tagagctccg tgtgctcggt tccaagatga gtggccacgg
     3241 cttttgcctg cggggcctca ttgtactcat catcatggaa gccgatcgag aaggtcttga
     3301 ccggacgaga ggattgggct tgcatcagcg ccaccacggt tgaagaatcg attccgcccg
     3361 atagaagcgc gccgagcgac acatccgaga gcatctgtcc cttgatcgcg cgacttaaca
     3421 gccgatgcag ttcatcctca gccgccatat ctgtccactc ggactcgatg gcctgtccgg
     3481 caaccgccgc cgcggaccaa tagggtttgg gttcagacca atagcgggcc gggcattcgg
     3541 aaggaatcgt cacatacgtt ccaggtaaga gctttcgaat tccggtgtag atcgagaagg
     3601 gtaacggcac ataagcatag cgcatgaaca aggccagggc atcccgatcg atctcaccgc
     3661 gccagcctgg ataagcttcc aacgccttca gctccgaggc aaagatgaac gttccgttag
     3721 accacccata gtagagcggc ttctctccga tccgatcccg agccagaact aggcgccgct
     3781 caatgcgatc ccatagcgcc aatgcaaaca tgccgacagt ggcctgaagc gctttttcca
     3841 caccccaggt ttcaaaggcc gttaacagcg tctcggtatc cgaatgccct cgccacggca
     3901 ggcccgtgag ctgccctcgg agttccagat gattgtagat ttcaccgttg aagacgatga
     3961 catatcgacc ggaagccgat tccattggct gatggccggc gggagacaga tcgaggatcg
     4021 acaggcgggc atggccaagg gcaagcccgt ggtcggcatc gcaccacagt cctgaatcgt
     4081 cgggaccgcg ataacgaatc atctcgacca tttttgaaac aatagggctg agctcagcat
     4141 cccgacagaa catcattccg gcaatgccgc acatcagtat gccgaacgaa ctgaatggtc
     4201 agacatgaag gaccgtatgg tcgcgcgcca accctgccca caccgattgc cactggtctt
     4261 tctctgcttg gcccgcggaa ccccaagatc tgcgggaccc gctcccaacg gtgcgcccca
     4321 actgaagggc caggtctctt gcaagagcgg cgctatgcac accaaagccc atcatcgccc
     4381 acctcaatga ggaaaggtcg cgcaccctct cgaccaggcg tcgacaatag ttgagccgac
     4441 cacggcccaa tctcgccgct gcgatttcag ccacacggta gcccaagagg acttcctcca
     4501 acgccgcaaa gcgactgtga gaaaagcttt gaagaaggag tgcttgatct tcgcaccgag
     4561 tatcttcgtc ggaataggga tgcgataaga accaggcccg cttgcccatc catgtcggat
     4621 gggcaagcgg aaacccccac catggtctcc ggcaaatggc ctcgtgtgag ccggccgttg
     4681 gatagcagcc gatgatctcg ccttctccct tgaagatcac agcgccggtt cccagcagat
     4741 cgatgtcacg atgctcttca aggaactgaa cctgccgttc aagacggtga ggatacgcca
     4801 cgtcgtcggc atccattctg gcgacatact gtcccctcgc gagacgcaca cattgattca
     4861 accgtgcagc taagccccta ttccctgatg gttcttgaat gaatcggatt cgaggatcct
     4921 gcgcatggaa cgacacgatc tctcgtgtcg aatcggtgga gccgtcatcg acgacgatga
     4981 gttcccagtt ttgataggtt tgggtcaaaa tcgaccgcaa cgcaaggtca atcgtcgcgg
     5041 acgcgttgta cgccgacatc gcaacagtga ccaggggtgc gttcatttaa caaactgttg
     5101 atcttccgga ggcggcaggc cgtatggcag atgcccgcag gatgttcaaa aaggctttcc
     5161 agcgaggccg cagcgagaga agaggcgaag cgtacttttt gccgtacgtt gagcctctga
     5221 gcgatgcgag aacaaagctg gaagcctttt tcaacatcct gttaactttc cttgtaaaac
     5281 atccgccccg gccagccggc ggcactgtag gtccaggccc cccgtagacg ccaagacaac
     5341 tcaaaaacca gacgacgcgc ggctcgcggg ccgcgttttt ttccccaaac caaggatgcg
     5401 acgaacacca acaggaatcg gatcatacga agaaccggac tcgtgcggta atcccgtaca
     5461 gcggcttcga acccatccca tttaattgga attcgaggcc gtcggggaac acgttggggc
     5521 agctctcgca gcaactcctc gaagcgtcgc tcataggtat ggtcacgtcg cactcgttca
     5581 aaaccgcagc gcgcaacctc atcccgccgt tcaggacgag agagcagcga tttgacctgt
     5641 tggatcatct cgtcggcacc ttcgaatgcc agaatttctt ccccaacatg gaaataccga
     5701 tcgaggtccg gggccttctc agtcaggaga cagcccccat atcccggcac ttcgaagata
     5761 cgcgctttga tctgtcgact tgcaaaatca gaacgaccgt gggctccatc actgaaatta
     5821 aggctgatct gagattcgcg aaagatttcc cccatacgct tggtctcgat cggaccttcc
     5881 ggccatccat gaccgaaaca aaccacctcg atgccttcat gacgaagcct ggcgatcatg
     5941 gctcgacgat tcccatacgc tgcaccgaca aagctcacgg gataccggca ggctgacgcg
     6001 ggaagggagg aaatcaaggt gtcgccgttg gccgcccact gagagaggca gaccgagttg
     6061 atgccgtcat gatggtaccg ttcacccatc tgcgggtagg tcgtcacgaa gagatcgaat
     6121 ttcggaccga tcagcttgga aaacatcgga tacttccagg aatcatcagt cgcccaattg
     6181 acgacccagg cgccgctatt gcggatcacc tggatcgtct cgatccacac ttcatagtgc
     6241 atcaggacgc agaagacgac atccgggctc gtttcctcca cacgtttcaa caacgcatga
     6301 ttcaggcgag aaaaatcttc gtacggtttg cgggacaacg actcgaagaa agtcacctca
     6361 tgtcccaaac ggcgcaaggc aggaataaaa ttcgcatact cgggtccttc tccgcgcgca
     6421 gggtcgccat aggcatgctc tcccaataca cacaagattt tcatgcgatc acgacgactt
     6481 agcccacatt attcatgaac acttctgctg caattgtgga ttcgccgctg cgacaagcca
     6541 acatgcgact tcgacggcaa gttggcgggc gggctcgccg ctcggtcggt caacagactg
     6601 tttcaagtct gcctcccttc ctcgcggctc cgcgcgcccg tctcgcttgg caactccgca
     6661 atttcgcgac gaactgtcat gaataatgtg agctagcccg tcgtgccgtc acgtggtacc
     6721 cgactgtaaa gcgccgttcc ctgtcgaacc tgtccagcca tttgcagaat atagggacga
     6781 tcaatccgag gataggaaag agcacgatcc acatcggaaa ccacagcagc ctgaccccta
     6841 ccgatagact cgtactccac gggctggtcg tttgtatgaa ccgtatggat tcctgcccaa
     6901 aaaatcgaaa cgacccttcg ttcggttcaa tgtggacttc ctcaaatccg gcagccttca
     6961 gaaatcgctc gtaccaccag ggtgtatagc cgccgtagta atggtatggc tcttgatgaa
     7021 tgcctgatcc aagcggcgcg gtgagcacaa gcctcccccc cggctgaaga atcctcgcca
     7081 attcgtggac cactcggatg ggctccggat gatgctccag cacctccgtg cagagcacca
     7141 tcccaaagct gccgtcggga accgggatgg ccgtcacgtc cgacacgaag tcgagtgccc
     7201 catagccgcc atatcgaagc tggtcgcctc gcagcggggc gagatcctga gtacgataat
     7261 cgcagtgggc gaacaacgca cgataggggc aggagccggc accgacatcc agcaccttcg
     7321 tcccactcgg caccaacgcc gcctgctgcg cgatccaacg gtcgcgccgc acggcgttat
     7381 aggcgagcag ttcactctga gccagccagg cccgtagacc gctactcacg cgccttcaca
     7441 aattcgaaaa gaccacaccc atactcgagt gaagctgccg catccgagac gacgaccttc
     7501 aataaccaag gtgccttgca agcagcggct gtgcggagcg acacccttgc gctagacttc
     7561 ataaattcgg atggcatcat cgatgatctc aagctttcga ggagtggatt gtgcatcgat
     7621 aaaaagttgc aactgctcaa gggtcggata agccgaatcc gcaccgaaga gtcttgcatc
     7681 atcaattaca atcacatgcc ctcgtatcgg gtgacggaaa atatgctcga gctcaagaag
     7741 tatcggcgaa tgctgatcac cacaggctgt aataccaccg gaataatgag cgtctagcca
     7801 gaacagtgtt gattgcttga ttgatgccag ggcgagaggc agcattgatg cgctatttcc
     7861 atgaagaatc ctg
//
LOCUS       DATMTI010001105         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_169282, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001105 DATMTI010000000
VERSION     DATMTI010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169282"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VN039_17480"
     CDS             complement(<1..1321)
                     /locus_tag="VN039_17480"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWV47792.1"
                     /translation="MAKRIHTTGRQRSDKAARTPGKVVIRQLPTGIPGLDQILGGGLP
                     EFSFNIIAGGPGCGKTTMAHQLVFANATKQRPALYFTVLGESAIKMLRYQQQYTFFDP
                     GKLNGAIRFINLSQVVLEQDLSAVLEAIVHAVEDVGPSIVVVDSFRTALRKRTDLTEM
                     DVQFFVQQLALHLTTWEATTFLIGEYVEAEIQENPIFTVADGLFWLSQVTERNSVVRK
                     LQILKLRGQASVPGLHTFRITKDGLQTFSRTLGLSHRKPRPGKRPRLSIGIPELDQMM
                     GGGIPLGDSLLMAGSSGTGKSAFGTTFIAEGLRQGEPGIMAVFEERAEDVKRRAANLG
                     QDLQKAELDGTLKILYLRPLDLSVDESLQAILDSIQEINAKRLVIDSLVGFEMALAPG
                     FRTDFRESLYRMMASVTGAGVTVLSTVELAEDFTEFRFSPYMVSFLAD"
     gene            complement(1336..1758)
                     /locus_tag="VN039_17485"
     CDS             complement(1336..1758)
                     /locus_tag="VN039_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47793.1"
                     /translation="MPYRVTPQVKDFARRLMLCEAAAATPTGTPQSAAFRVCGKLRQP
                     LSRLAGVAGFRSLLSRALALTHDDVRWMKAVHVTAEGYLEGLDESQARLSQDAIAEGE
                     AVLIAHLIGLLVTFIGAELTVRLVQDEWPDAAFDDLEA"
     gene            complement(1958..>2246)
                     /locus_tag="VN039_17490"
     CDS             complement(1958..>2246)
                     /locus_tag="VN039_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV47794.1"
                     /translation="ELENVIERAVTLSRGEKISPDDLPSAVQGARGDRRILDEAAEHT
                     LPLHQLEKEYIKKVLEKTGGNKYQAAHALGIDRKTLYRKLAEIESRPPSEE"
BASE COUNT          464 a          672 c          629 g          481 t
ORIGIN      
        1 catcggcgag aaacgacacc atataggggc tgaagcgaaa ttccgtgaag tcctcagcca
       61 attccaccgt gctcaacacc gtcacgccgg cgcctgtcac ggaagccatc atccggtaca
      121 acgattcccg aaaatctgtg cggaagccgg gcgccagcgc catctcgaag ccgaccaatg
      181 aatcgatgac cagccgcttg gcattgatct cttggatcga atcgaggatt gcctgcaggc
      241 tttcatccac cgagaggtcc agcggtcgca ggtagagaat tttaagtgtc ccgtcgagct
      301 cggccttttg caaatcctgt cccaagtttg ccgctcgccg cttcacatct tccgcccgtt
      361 cttcgaacac cgccatgatg cccggctcgc cttggcggag gccttcggca atgaaggttg
      421 tcccaaatgc cgattttccc gttccggacg accctgccat cagcaggctg tccccaaggg
      481 gaatgcctcc gcccatcatc tgatccagct cgggaatgcc gatggacagg cgcgggcgtt
      541 tgccgggcct gggttttcgg tggctcaatc ccagcgtgcg tgagaaggtt tgcaggccgt
      601 ccttcgtgat ccgaaacgtg tgcaatcccg gcacggacgc ctgaccgcgc agcttgagga
      661 tctgcaactt gcgcacgacc gaattgcgtt ccgtcacctg cgagagccag aagagcccgt
      721 cggccacggt gaagatcggg ttttcctgga tttcggcctc gacatactcc ccgatcagaa
      781 acgtcgtggc ctcccacgtg gtcaaatgca gggccaattg ctgcacgaag aattgcacat
      841 ccatctcggt gaggtccgtt cttttgcgca acgcggtgcg gaaggaatcg actaccacga
      901 tgctcgggcc gacgtcctcc accgcgtgga caatcgcctc caacaccgcg ctcaaatcct
      961 gttcgaggac gacctggctc aagttgataa agcgaatggc gccgttcaat ttgccggggt
     1021 caaagaacgt atattgctgc tggtagcgca gcatcttgat ggcggattcg ccaaggaccg
     1081 tgaaatagag cgccggccgt tgcttcgtgg cattggcgaa cactaactgg tgcgccatcg
     1141 tcgttttgcc gcagccgggg cctcccgcga tgatgttgaa ggaaaattct ggaaggcctc
     1201 cgcccaagat ctggtccagt ccgggaatgc cggtaggcaa ctggcggatg acgactttgc
     1261 ccggtgtgcg ggcggcctta tccgagcgtt gacgtcctgt cgtgtggatt cgtttagcca
     1321 tggattgtcc ttctattaag cctccaaatc gtcgaacgcc gcgtcgggcc attcgtcctg
     1381 caccagacgc acggtcagct ccgccccgat gaacgtcacg agcagcccga tcaagtgagc
     1441 aatgagcaca gcctctcctt ctgcgatcgc atcttgggac agacgcgcct gcgattcgtc
     1501 caatccctcc aagtatccct ccgccgtgac gtgaaccgcc ttcatccaac gcacgtcgtc
     1561 atgagtcaat gccaatgcgc gggaaagaag cgagcgaaac ccggccaccc ccgcaagtcg
     1621 tgagaggggt tggcgcagtt tgccacagac gcgaaaagcc gccgactggg gtgtacccgt
     1681 aggtgtggcc gcagccgcct cgcacaacat gaggcggcgc gcaaaatctt ttacttgtgg
     1741 agtgacgcgg tacggcatcg gcgaaccttc gcgaatcacg agtggtccgc cagtctagca
     1801 tggagaatgt ctcctatatc atgatattct gagcatcaac aatgcttata acagcccaca
     1861 tgcagaggcc taggactcac cctagggtct ctacttgtcc tgccatgcgg agaaaggaaa
     1921 atagatgagc caacagaacc tgccaggaat gatcgagtca ttcctccgag gggggtctac
     1981 tttcgatttc ggcgagttta cgatacaggg tctttcggtc gatgccaagg gcatgcgcgg
     2041 cctgatattt attgccgccg gttttttcga gaactttttt gatgtattct ttttcaagct
     2101 gatgtaaagg cagagtatgc tcggcggctt cgtccagtat gcgccggtcg ccacgggccc
     2161 cttgcactgc cgagggtaga tcatccggag aaatcttctc gcctcgactg agcgtcacgg
     2221 cccgttcgat gacgttctcc aattcc
//
LOCUS       DATMTI010001106         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_678363, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001106 DATMTI010000000
VERSION     DATMTI010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678363"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1381
                     /locus_tag="VN039_17495"
     CDS             119..1381
                     /locus_tag="VN039_17495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV47795.1"
                     /translation="MSQGSLVSQATRKIAGKVLSHGIGRLAERVRTNGGQPLDLILPD
                     GSRLNFGQEPRVALAIRDPNLLSALTHPTLGLLGEAFVDGRIDIDGDIISLIASVERL
                     VAASGSPATVRIPASSACHTPKQDFDDIKHHYDVSNDFYRLWLDDRMVYSCAYFRTGE
                     ETIDDAQEAKLDHICRKLRLQPGERFLDIGCGWGGLVIHAAQRYGVHAVGITLSDNQF
                     ALAKERIKTSGLERQIRVLLLDYRDAPERLGESTFDKISSIGMFEHVGRDNFHLYFGI
                     VRRLLRDRGLFMNHGITSPDADGRTVGSGVSEFMDKYVFPHTELPHLHSVIREMAKQN
                     FEVYDVESLRPHYARTLAHWSRRLERQLTSARHFVGERTLRIWRTYLAGSSLAFSQGW
                     LTVYQVLASRQEAGGSTELPLTRAWMYH"
     gene            complement(1438..2886)
                     /gene="hemG"
                     /locus_tag="VN039_17500"
     CDS             complement(1438..2886)
                     /gene="hemG"
                     /locus_tag="VN039_17500"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HWV47796.1"
                     /translation="MTRLRTVVIVGGGISGLATAFSLQEMATKAGLPIRCTILESESS
                     WGGKIVTHRVGEIVTEAGPDSFLSQKQAGLDLCMKLGLADQLINTNETAKKASVLYAG
                     RLHDLPEGLLSFVPKQLGSFFRSGLLTWTGLARMGLEVAVPPGPSTGDESLAAFLRRR
                     FGVQAFTRVLEPLMAGIYAGDAEHMSLRATFPRFFELEQQHGSIVRGMMAAKKAVLSV
                     SIDQPRRTMFVSLKNGLSDLVTALASRLTQQGVELRVGARVEALRVRSHEIGRWMYDL
                     ILQDGSALSADGVVLATPAYVSAELLRPLTPIAGGLLDMIPYASTATIAMTFPRTLTS
                     AIEGFGFIVPRTEQRHLIAATWTSLKWSYRAPADQLLVRCYVGGVGREDILQLDDDAL
                     TATVREELAAVCGIRVGPSYAEVNRWWKAMPQYTLGHLNRLAQLEAALSRYPGLVLTG
                     AGYRGVGIPDCIRDGAVAGERVIQDLLGETRR"
BASE COUNT          694 a          911 c          806 g          625 t
ORIGIN      
        1 gcatctttga cgttcgcttg agccaaattt atcgaacggt gagtatttca gggcttggcc
       61 tccaactaat acccgtattc gatcaagaat cgaagagtgc agcccaaagg aggttatcat
      121 gtctcagggc agtctcgtct cgcaggcaac ccgaaagatc gcaggcaagg tactgagtca
      181 tggtattgga cggctggcgg aaagagtccg gacaaatggc ggccagccgc tcgacctcat
      241 tcttcccgac ggttcgcgtc tcaatttcgg ccaggaacct cgcgtggcac tcgcaatccg
      301 cgatccgaac ttgctgtcgg cattaaccca tcccacgctc ggattgctcg gagaagcgtt
      361 cgtcgatggg cgcatcgata ttgacggcga catcatctcc ctgatcgcca gcgttgagcg
      421 cctcgtggcg gccagcggct cgccggcgac ggtacgaatc ccagcttcat cggcatgcca
      481 tactcccaaa caggatttcg acgatattaa acatcactac gacgtgagca acgattttta
      541 ccgactctgg ctggatgacc gcatggtcta ttcttgcgcg tattttcgaa ccggggagga
      601 aacgatcgac gacgcgcaag aggccaagct cgatcacatc tgccgcaaac tacgccttca
      661 acccggtgaa cgtttcctcg acatcggctg cggttggggc gggctggtta ttcatgccgc
      721 tcagcgttac ggggttcacg cggtcggaat cacgctctcg gacaatcagt ttgccttggc
      781 caaggagcgt atcaagacgt caggccttga acgacagatc agggtactcc tgctggacta
      841 tcgcgacgcg ccggaaagat tgggggagag caccttcgac aaaatttcca gtatcggcat
      901 gtttgagcat gtcggccgag ataattttca cctctacttc ggcattgtgc ggcgcctgct
      961 gcgcgaccga gggctgttca tgaaccatgg catcacctca ccggatgccg acggacgcac
     1021 ggtcggctcc ggagtgagcg agtttatgga caagtacgtg ttcccacata ccgaactccc
     1081 ccatctccac tcggtcatcc gagagatggc gaaacagaac ttcgaggtct atgacgtcga
     1141 aagcctacga ccgcactatg ctcggacact cgcacactgg tcacgcagac tggaaagaca
     1201 gctcacctca gcccgtcatt tcgtcggcga acgaacgctt cggatatggc gaacctatct
     1261 cgcaggctca tcactggcat tttcgcaggg ctggctgacc gtctatcagg tgctggccag
     1321 ccgccaggaa gccggcggct cgactgagct gccgttgacc agagcctgga tgtaccactg
     1381 aaactcaacc tgcatccccg accttagcct taggctcagc ttatccccat ggtgagatca
     1441 tcgtctggtt tcgcctaaga ggtcctgtat aaccctctcg cctgccacag ctccatcacg
     1501 aatgcagtcg ggtatgccga ctccgcgata accagctccg gtaagaacaa gtccgggata
     1561 gcggctcagt gccgcctcca gttgtgccaa ccgattcaga tggccgagcg tgtactgcgg
     1621 catcgccttc caccatcgat tcacttcggc ataactcggt cctactctga tgccgcatac
     1681 agcggcgagt tcttctctga ctgttgccgt caaggcatcg tcatccagct gaagaatatc
     1741 ttcccgccct actccaccga catagcagcg caccaacagc tgatccgccg gagcccgata
     1801 cgaccacttg agcgaagtcc acgttgcggc aattaaatgc cgctgctccg ttcgcggcac
     1861 gataaatccg aacccttcga tggcactcgt cagagttcgt ggaaatgtca tcgcaatggt
     1921 ggcagtcgaa gcataaggaa tcatgtccag caaaccgccg gctatcggcg tcaatgggcg
     1981 taagagttca gcggaaacat aggcaggtgt ggcaaggacc acgccgtctg cagaaagcgc
     2041 cgatccatct tgcaaaataa gatcgtacat ccagcggccg atctcatgcg acctcactct
     2101 gagggcctcg acacgagccc ccacacgcag ctcgaccccc tgctgcgtca gccgactcgc
     2161 caaggcagtc accaaatcgc tgagaccgtt ctttaaactg acgaacatcg tccgtctggg
     2221 ctgatctatc gatactgata aaaccgcttt cttggcggcc atcatgccgc gaacgatact
     2281 gccatgctgt tgctccaact caaaaaaccg cgggaacgtg gcgcgcagac tcatgtgctc
     2341 ggcatcaccc gcgtaaattc cggccatcaa cggttccaag acccgcgtaa acgcttgcac
     2401 cccaaaccga cggcgaagaa aggcggccaa cgattcatca cccgttgagg ggcctggtgg
     2461 aacagcgacc tccagcccca tacgcgccaa ccctgtccag gtgagcagac cgctccgaaa
     2521 gaaagacccc agttgtttcg gaacgaaaga gagcagtccc tcgggcaaat catgcagccg
     2581 tcctgcatag agcacgctcg ccttcttggc cgtttcattc gtattgataa gttgatcggc
     2641 aaggccaagc ttcatgcaga gatcgagacc ggcctgtttt tgggaaagaa aggagtccgg
     2701 tccggcttca gtcacaatct cgccgacccg gtgcgtgacg atctttcctc cccaggacga
     2761 ctcggattcg aggatcgtgc agcgaatggg aaggccggcc ttggtcgcca tctcttgtag
     2821 tgaaaaagca gtggccaagc cggaaatacc gccgcccact atcacgactg tgcgtagtcg
     2881 agtcacgact ggatgcagag agaggattcg tgtgccgtca agacgtcgtg taacgtctcg
     2941 atgagtgcgg gtgagtcatt cagcatggct atacgctcaa ggtgtagacc cttgttggtc
     3001 gccaactgct tcaattcaat atcaatatca aagaga
//
LOCUS       DATMTI010001107         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_41273, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001107 DATMTI010000000
VERSION     DATMTI010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41273"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VN039_17505"
     CDS             complement(<1..566)
                     /locus_tag="VN039_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47797.1"
                     /translation="MALPASDSKNVWPPKPMENIFAHMGQWSAWYSNDISKLQAAYGG
                     GLQADSTGFFASDKGGFKPTIGQRIQRFFVGQPTLGPNRNTKLPVPIAGLVCQAVGDL
                     LYADPPTFTVRVDTDKDGSGVPKAQANPTQERLNQLADEGMYTTLARGAEVGAALGGH
                     FLRVAWDKSVVADRPFLDVVDADQALPE"
     gene            complement(628..>1979)
                     /locus_tag="VN039_17510"
     CDS             complement(628..>1979)
                     /locus_tag="VN039_17510"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HWV47798.1"
                     /translation="SRTLAKVSLHEATTRPNILILVVSAGDEAAKRLLADCVALANAS
                     DALRGSIVDDGKSLLTLTNGSTIRSVPASIRQIRGWPVDVLIIDEAGFIDNEIWDAAL
                     PAIISRPGSRVVIASSPWGSSEHWYRALWNRGMDSPDENYRSWQWSSYDSPLADKGLL
                     DELKDARPSEWFAREVLGEFTEESGSYFSERELMDAVADYELCAPDDLEWWTDQRFAA
                     AGGIDWGFSHDANALTLVSVLEDFGKNREILGDKLPLFIPWFFYKYRWAYTDFIDYVV
                     SVAGKYFLPVVASEVNGVGQYPTTMLDDKMSEAGFFSAVAPVVTDVRRKQSGFGMIKG
                     LLQSRRLVLPRDPELLKQLRGLEFEQLPGGSLRIAVPDRVGHDDVAMSFMQAVSSIRL
                     DQAVRRSTGFGESKLIPADEEFVQTKSGLQVPVRARPVSFHRDAFMYPRGSEAGEGW"
BASE COUNT          558 a          676 c          419 g          326 t
ORIGIN      
        1 aactcaggca acgcctgatc cgcatcaacc acatccaaaa acgggcgatc cgcaacaaca
       61 ctcttatccc acgcaacccg cagaaaatgg cccccaagtg cggcaccaac ctcagcacca
      121 cgcgccaaag tcgtatacat gccctcatcg gcaagttggt tcaaacgttc ctgcgtcggg
      181 ttagcttgcg ccttcggaac cccggaacca tccttatccg tatccacccg gacagtgaac
      241 gtgggcgggt ccgcatacaa cagatcacca acagcctgac aaaccaaccc agcaatcggc
      301 accggcagtt tcgtgttacg gttcggcccc aacgtcggct gaccaacaaa aaaccgttga
      361 atacgctgcc caatagtcgg cttgaaacca cccttatccg acgcgaaaaa cccggtactg
      421 tcagcctgca aaccgccacc atacgcagcc tgcaacttag agatatcgtt cgaataccag
      481 gccgaccatt gccccatgtg agcgaaaatg ttctccatcg gcttcggagg ccacacgttt
      541 ttgctgtcgg atgcggggag cgccattagt accccttccg ggcctttaaa cacgaaaagc
      601 ccccgcaggg gccacacaag aaacgaacta ccagccttcg ccggcctctg aaccgcgcgg
      661 atacatgaac gcgtcacgat gaaacgacac cggacgcgca cgaaccggaa cctgtaggcc
      721 cgactttgtt tgcacgaact cttcatcagc cggaatcagt ttcgattcac caaacccggt
      781 agaacgacgt acggcctgat ccaaacggat agacgaaaca gcctgcataa acgacatcgc
      841 aacatcgtcg tgccctacac gatccggcac agcaatccgc aacgacccac ccggcagttg
      901 ctcaaactca agcccacgca actgcttcaa caattcaggg tcacgcggca acacaagccg
      961 tctgctttgc agtaaaccct tgatcatccc gaaaccggac tgcttacgcc gcacatccgt
     1021 cacaacaggg gcaacagccg agaaaaaccc ggcctccgac attttgtcat ccaacatggt
     1081 cgtcggatac tggcccacac cgttgacctc agacgccaca accggaagaa aatacttccc
     1141 agcaaccgac actacgtagt cgatgaaatc tgtgtacgcc caccggtact tgtagaaaaa
     1201 ccagggaatg aacagcggca gtttgtcgcc aaggatctca cggttcttac cgaaatcctc
     1261 aagaaccgaa accagtgtca gcgcgttcgc gtcatggctg aaaccccagt caataccacc
     1321 cgcagccgca aaccgttgat ccgtccacca ctcaaggtca tccggggcgc acagttcata
     1381 atcagcaacc gcatccatca gctcgcgttc gctgaaatac gaacccgact cttccgtgaa
     1441 ctcgcccaaa acctcacgag caaaccactc agacggacgc gcatccttca actcatcaag
     1501 cagaccctta tccgccaacg ggctgtcata agacgaccac tgccacgacc tgtaattctc
     1561 gtcaggagaa tccatccccc gattccacaa agcacgatac caatgctccg acgaacccca
     1621 cggactagaa gcaataacca cccgagaccc aggacgcgaa ataatcgccg gaagggcagc
     1681 atcccaaatc tcattatcaa taaacccggc ctcgtcaata atcaggacat caacaggcca
     1741 accacgaatc tgacgaatcg acgccggaac cgaacggatc gtcgaaccgt tcgtcaaagt
     1801 cagaagtgac ttaccatcat caacaatcga cccacgcaaa gcatcagacg cattcgccaa
     1861 cgcaacacaa tccgccagaa gccgcttagc agcctcatcc cccgccgaca caaccaggat
     1921 caggatattc gggcgcgtcg tcgcctcatg caacgaaacc ttcgccaacg tccgcgact
//
LOCUS       DATMTI010001108         1545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_125916, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001108 DATMTI010000000
VERSION     DATMTI010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125916"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1545
                     /locus_tag="VN039_17515"
     CDS             <1..>1545
                     /locus_tag="VN039_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47799.1"
                     /translation="QAFSHSLRGLPSGLLVLPSDNQFSPFSTDVAIAFYGNDPLRYRS
                     RTRSANANLTLNGNFGSWTANFNARHSESNGISNSQRQIFASNGLPTNVNPFTADLSS
                     FVQITTDRTSSRSLTNLAQLSMTGPLASVPAGSIQTTIEAHLEWNHLRSSSSFSTFGN
                     RDVSRAEQAIRGAVDIPLTSRAQGVLPQIGDWDATAEYQFDHFSDAGTLHDYAFGLTW
                     QPASTLRFHGAIEHRETPASVDLLGSPNLEVPNVSVFDPLTGNTVQVVEITGGNPSLK
                     PETDQIRRLSTQLTLVKRLNLQLNGEYTDTNRRNFVSSLPDESAAIMLAFPDRFVRDS
                     NGNLTTIDLRPVNFASDEEKRLRWGLSMNARLGGAAPGQKYQPGRVTPPTFLQLTANH
                     SIVFSDRIVIRSGLDPVDLLSGGAIGIGGGRVRHQVDGTAAITSGGLGARIGVMWRSR
                     SSLLTKVNDVADTLSFSPLMTINLRAFADTRRILPQVKWAKGLRLSVDVLNLTNSRQR
                     VRDSFG"
BASE COUNT          318 a          497 c          412 g          318 t
ORIGIN      
        1 tgcaggcatt cagtcattcc ttacgcgggc ttccgtcggg tttgctggtg cttccatcgg
       61 acaatcagtt ttcgcccttc tccaccgatg tggccattgc cttctacggc aacgacccgc
      121 ttcgctatcg ctcccgaacg agatccgcca acgcgaacct gacgctgaac ggaaatttcg
      181 gctcgtggac cgccaacttc aatgctcgac attccgagtc gaacggcatc tcaaattcgc
      241 aacggcagat atttgcctcg aacggacttc cgaccaacgt caaccctttc actgcagacc
      301 tcagttcatt cgttcagatc accaccgacc gaacttcgtc acgatcgctt accaatctcg
      361 cgcagctgtc gatgaccggt ccgcttgcaa gtgtccccgc agggtccatc cagaccacga
      421 tcgaggcgca tttggaatgg aatcacctac gatcgtccag cagcttctct acatttggca
      481 accgcgacgt cagccgggca gaacaagcga tccgcggagc cgtcgacata ccgctgacca
      541 gccgagccca aggcgttctt ccgcagatcg gcgattggga tgcgaccgcc gaatatcagt
      601 tcgatcattt ttccgatgcc ggaacgcttc acgattatgc gttcggcctg acgtggcagc
      661 cagcttccac tctccggttc catggtgcga tcgaacatcg agagaccccg gccagtgtcg
      721 accttctcgg cagtccgaac ctggaagttc cgaatgtatc ggtgttcgat cccttgaccg
      781 gcaacacggt gcaggtggtg gagatcaccg gtggaaatcc ctcgctcaag ccggaaaccg
      841 accagatccg ccggctttcc acgcagctaa ccctcgtcaa gcgactgaac ctgcagctca
      901 acggggagta taccgacacc aaccggcgta atttcgtttc ctcgcttccg gacgagagtg
      961 cggcaatcat gcttgcgttc cccgaccggt ttgtccggga ttcgaacggc aatctcacga
     1021 cgatcgatct gcgaccggtc aacttcgcgt ccgatgaaga gaaaaggctg cgttggggcc
     1081 tcagcatgaa cgcccgactc ggaggcgctg cgccgggcca gaaatatcag ccgggccgcg
     1141 tcactccgcc gacctttttg caactaaccg ccaatcattc gatcgtcttt tccgacagga
     1201 tcgtcatccg atccggcctc gacccagtcg atctcctaag cggcggagcc atcggcatcg
     1261 gtggcggtcg ggtccggcat caggtggacg gaacagcggc aataacctcc ggtggactag
     1321 gcgcacggat cggagtgatg tggcgcagcc gaagttccct gctgaccaag gtcaacgacg
     1381 ttgccgatac tctcagtttc tcgccgctga tgaccatcaa cctgcgggca ttcgccgata
     1441 cgaggcgtat tcttccgcaa gtgaagtggg ccaagggcct tcggctttcg gtcgacgtgc
     1501 tcaacctcac caacagccgg caaagagtgc gcgattcctt cggga
//
LOCUS       DATMTI010001109         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_678599, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001109 DATMTI010000000
VERSION     DATMTI010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678599"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VN039_17520"
     rRNA            complement(<1..815)
                     /locus_tag="VN039_17520"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1078..1153)
                     /locus_tag="VN039_17525"
     tRNA            complement(1078..1153)
                     /locus_tag="VN039_17525"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1118..1120),aa:Ala,seq:tgc)
     gene            complement(1302..1378)
                     /locus_tag="VN039_17530"
     tRNA            complement(1302..1378)
                     /locus_tag="VN039_17530"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1342..1344),aa:Ile,seq:gat)
     gene            complement(1559..>2057)
                     /locus_tag="VN039_17535"
     rRNA            complement(1559..>2057)
                     /locus_tag="VN039_17535"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          451 a          623 c          499 g          484 t
ORIGIN      
        1 acccctaaac acaactcatc cggtaccttt tcaacggtaa tcggttcgag cctccagtgc
       61 gtgttaccgc accttcactc tggtcatgcc tagatcgccg ggtttcgggt ctaatgcatc
      121 aaactcaatc gccctattca gactcgcttt cgctgcgcct acacctaacg gcttaagctt
      181 gcttgataca ctaagtcaca gacccattat gcaagaggta cgcggtcagg tctcaaggac
      241 cctcccactg cttgtaggca tccggtttca ggtactgttt cactcccctc atcggggtgc
      301 ttttcacctt tccctcacgg tactggttcg ctatcggtca tgtacgagta tttaggcttg
      361 gagggtggtc cccccatgtt cagacagggt ttcacgtgcc ccgccctact caagtcctgt
      421 tgtttcgctt tcgcatacgg gactgtcacc cgctatggtc aaactttcca gaatgttctg
      481 ctaactaaac aacaggcact ggcctggtcc gcgttcgctc gccactacta acggaatctc
      541 ggttgatgtc ttttcctcca ggtactgaga tgtttcagtt ccccgggttc gcttcaccaa
      601 agcctatttt attcagctta gtgatacctc tcccatttaa ctacgcgacc ggcgaaccga
      661 taacggaatt aaatggtgaa ggtgggttgt cccattcgga aatcgcggga tcaaagcttg
      721 ctcacagctc ccccacgctt atcgcagcgt gccacgtcct tcatcgcctg tacatgccaa
      781 ggcattcacc agatgccctt acctcacgct tgagagtcca caccaccaat gacagcactg
      841 gagaagcact gcgcatcagc ttgtattcgg tgtggtgatt taactcagcc agataatcat
      901 tttgtgtaca acaccgctcg cttccatccg acccgaagat cgtgtgaaac cgaaccatgt
      961 cgccacggca tcgattaaaa aacccattca caatgtcaaa gaagcctgca tcagcaagcc
     1021 atatcgccgg acgtatccgg caaaccgcta ctcttcatct ctagagaatg ctgtggttgg
     1081 tggagcctat cgggatcgaa ccgatgacct gatgcttgca aagcaaccgc tctcccagct
     1141 gagctaaggc cccgtaacca ttccgctgtt gcttgacgcc aacgaaggca aagccttcgc
     1201 aaggccgacc ggccgccgcc gcttatgcgg cgagtagcca agaacgcgga tgcgttcggc
     1261 ggcgcttgag cacccaacaa gcaaaccatt tgcaaagcaa atggtgggcc ggggaggagt
     1321 tgaacctccg acctcacgct tatcaggcgt gcgctctaac cacctgagct accggcccag
     1381 cgcttcgcag gccgcttttc agcagcgcga ggcgcttata gcctgctcag ctatcgctac
     1441 agccttgcag ctgtaacaat ctctagtgat gaagggacat gaggacggcg gctatgttct
     1501 ttggaaatga cgaagctctt ctggatgcta gatccagcgc tttcggcacg atccttagaa
     1561 aggaggtgat ccagccgcag gttcccctac ggctaccttg ttacgacttc accccagtcg
     1621 ctaaacccac cgtggtcgcc tgcctctctt gcgagttagc gcagcgcctt cgggtgaatc
     1681 caactcccat ggtgtgacgg gcggtgtgta caaggcctgg gaacgtattc accgcggcat
     1741 gctgatccgc gattactagc gattccgcct tcatgctctc gagttgcaga gaacaatccg
     1801 aactgagaca agttttggag attagctcac cctcgcggga ttgctgccca ctgtacttgc
     1861 cattgtagca cgtgtgtagc ccagcgcgta agggccatga ggacttgacg tcatccccac
     1921 cttcctccgg cttatcaccg gcggttcctc tagagtgccc aactgaatga tggcaactaa
     1981 aggcgagggt tgcgctcgtt gcgggactta acccaacatc tcacgacacg agctgacgac
     2041 agccatgcag cacctgt
//
LOCUS       DATMTI010001110         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_41497, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001110 DATMTI010000000
VERSION     DATMTI010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41497"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /gene="hrpB"
                     /locus_tag="VN039_17540"
     CDS             complement(<1..1511)
                     /gene="hrpB"
                     /locus_tag="VN039_17540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HWV47800.1"
                     /translation="MRLETKIGPTTKIEVVTEGVLTRLLQQDPSLEAYGIVLFDEFHE
                     RSLQADMGLALCLETQRLFRPDLRLFIMSATLDCGPVAALLGRAPLITCAGRMFPVET
                     RYLDQPLTGRLDAAVSQLIRRSLAKDHGSLLIFLPGMAEIRRVERLLMDSNLDQSVQI
                     APLHGDLPQDMQDVAIRPTAPGRRKVVLATSIAETSLTIDGIRVVIDAGLARIPRYDP
                     RSGLTRLETIRVTKDSAEQRRGRAGRLEAGICYRLWTEKEQAGLAPRRPPEILEADLT
                     PLMLDLAQWGTRDPSELSWLTPPPADAVAQARDVLVQLGAFSADGRPTAHARRMVELP
                     LHPRLAHMMLRAVPLGLADLACKVAALLSERDILHGPRERQNADLRVRLDVLGEQYDS
                     IGLEVNRATVDRVRRTAGLWRRRLSSEQSREAEGNGYDRSNSIGLLAALAYPDRIARR
                     QSDCEGRYRLVNGRGARFKTADPLAIEPFLVIADLDGRDQWSEINLAVPIAQK"
BASE COUNT          316 a          457 c          454 g          300 t
ORIGIN      
        1 tctttttgag cgatcggaac agcgagattg atctcggacc actgatcgcg tccatccaag
       61 tcggcgatca caaggaaggg ttctatggcc aagggatcgg cggtcttaaa ccgagcgcct
      121 cggccattca caagacggta ccgcccttca caatccgact gccgccgagc aattcgatca
      181 ggataggcga gcgcagctaa cagcccgatt gagtttgaac gatcatatcc attgccttcc
      241 gcctcgcgag attgttcact gctgagccgt ctccgccata ggccggccgt tcgcctcact
      301 cggtcaactg tggctcgatt cacctccaga ccgatggagt catactgttc ccccagaacg
      361 tccagcctga cgcgcaaatc ggcgttctgc cgttcgcgtg gcccatgaag aatgtcccgt
      421 tcgctcaata acgccgccac cttgcaagca agatcggcaa ggcccaatgg caccgcccga
      481 agcatcatgt gggctaagcg gggatgcagg gggagttcga ccattcgtcg ggcatgggcc
      541 gtcggccggc cgtcagccga gaaggccccg agttggacta atacatccct ggcctgtgcg
      601 actgcatcgg caggcggcgg agtgagccac gacagttccg acggatcacg tgtgccccac
      661 tgggcaagat ccagcatgag aggagtgaga tccgcctcga ggatttccgg tggacgacgg
      721 ggagcaagcc cagcctgttc tttttcagtc catagccggt aacaaatacc tgcctcaagt
      781 cgtccggccc ggccgcggcg ttgttcagca gaatcctttg tgacacgaat ggtctccaat
      841 cgagtcaggc cggagcgagg atcgtaccgt ggaatacgtg ccaggcctgc atcgatcacc
      901 acacggatgc cgtcgatcgt cagactggtt tcggcgatgg aagtggcaag cacgactttt
      961 cttcgcccgg gagccgtcgg tcgaatcgcg acatcctgca tgtcctgggg aagatcgccg
     1021 tgcagcggcg cgatctgtac agattggtct aggttggagt ccatcagtag ccgttcgacc
     1081 cgacgaattt cggccattcc cggcaggaag atcaacagac tcccatgatc ctttgcgagt
     1141 gatcgtcgga tgagctgcga tacggccgcg tcgagccgtc cggtaagcgg ctgatcaagg
     1201 taccgggtct cgaccggaaa catccggcct gcgcacgtga tgaggggagc tcgccccaac
     1261 agtgcggcaa ccggcccgca gtccaaagtg gcggacatga tgaagagacg aaggtctggg
     1321 cggaagaggc gttgagtttc gaggcacagg gcaagtccca tgtccgcttg cagacttcgc
     1381 tcgtggaact catcaaacaa cacgatgcca taggcctcca gcgacgggtc ttgttggagc
     1441 agcctcgtca gcactccctc tgtcaccacc tcgattttag tcgtcggtcc gatcttcgtt
     1501 tcgaggcgca tgcgatagcc gacggtt
//
LOCUS       DATMTI010001111         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_593087, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001111 DATMTI010000000
VERSION     DATMTI010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593087"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VN039_17545"
     CDS             <1..1051
                     /locus_tag="VN039_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47801.1"
                     /translation="AANKVFCGPQSGSDDLPAFRELVPSDLPVATTSSIGAVSVGSGL
                     SVTGGVLSADAPPIATTGSTGVISVGSGLSVTGGGILSANAPAIATTSTPGIVSVGDG
                     LAVTAGGVLSANSPPVATTSSTGVVSVGSGLSITGAGVLSRSTSYSVGVPALSSFTKV
                     NQGTSTVSEVSGSSITMSGVGSAAGTYNNFLLYKAAPSSPYRLAAYLSVGAQAVTYFG
                     GGLAFYDTSTTRFVEVFFCWNVNSVCNIQVSKWSSPTTFNSTPITWGWSMNSAGFWLG
                     LRDDGTNVYYEYSSDGANFVTIYSEPKSSGYLSSYSNVALVTNLSNATSSHMTNTTLF
                     IWDVNGLSRTPSIVG"
     gene            complement(1365..>1733)
                     /locus_tag="VN039_17550"
     CDS             complement(1365..>1733)
                     /locus_tag="VN039_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV47802.1"
                     /translation="FESGCVVPPRSSAHIVCSFTHPTGSNRSSHPLINLSKSLGPALM
                     TSWRDLGARRGKADSYGDRLRIEREMKGLAQRVRDNPEIERVLAQAAKAQGIDMKGRT
                     VAQEMERRIAKNERERGFDR"
BASE COUNT          367 a          502 c          434 g          430 t
ORIGIN      
        1 ggccgccaac aaagtatttt gtggcccaca atcgggttca gatgacctcc cggccttcag
       61 ggaactggta ccttctgatc tgccagttgc caccacttcc tcgataggag cagtcagcgt
      121 aggcagtggt ttgtctgtca ctggaggcgt cctaagtgcc gatgcgcccc cgattgcaac
      181 cacaggctcc accggtgtta ttagtgtcgg cagcggactc tctgtgaccg gcggcggcat
      241 cctcagcgcc aatgcccccg ccatcgccac gaccagcacg ccaggcattg tcagcgttgg
      301 tgacggatta gcagtaactg ccggtggcgt acttagtgct aactcgccgc ccgtcgccac
      361 aaccagcagc acgggcgttg tcagcgtagg gagcggcctc tctataacag gggcaggtgt
      421 ccttagccga agcacttcct attctgtggg cgtaccagca ttgagcagct tcacaaaggt
      481 taatcaaggt actagcacgg tgagtgaagt gtctggttcg tcgattacca tgtccggtgt
      541 cggttcggct gccggaactt acaacaattt tcttctgtat aaggcagctc catcttcacc
      601 gtaccgcttg gctgcgtatt tgtctgttgg agcacaagct gtcacctatt ttggcggagg
      661 attggcgttt tatgatacgt cgacgacacg atttgtcgag gttttcttct gctggaacgt
      721 gaatagcgtc tgtaacatac aggtttctaa gtggagctct ccgacaacct tcaattcaac
      781 tcccatcacc tggggttggt cgatgaattc tgcaggattc tggttaggtc tccgcgacga
      841 tggcacgaat gtgtattacg agtatagttc ggatggcgcg aatttcgtca caatttactc
      901 tgagccaaag tcatcaggct atctaagctc ctactcaaat gtggcactgg taaccaacct
      961 gagcaatgca acgagttcgc acatgacgaa cacaacgctg ttcatttggg acgttaacgg
     1021 tctgagccgt acaccttcaa ttgtcgggta gcttcataca ttgacatagc actaattgct
     1081 tgagacctgc ttgcggttcg cgccatgaat aaaatgcgca cgactcgcag gcaggattct
     1141 ctcatcccgc acgtgagggt cgcggatccg atttcaaata cccctgcccc cactccataa
     1201 cgccaggtgt catggctgtg aattcgtcgc tatgcacctg ccacccccat tcggcctcaa
     1261 tatctttaat tcttcgcgaa agccatttga tatgacctac tattggaaat cgaaaccgtt
     1321 gaaatttgca ggtttcaatt ggtccataca ccaatggtag tcagtcatcg gtcaaatcct
     1381 ctctcccttt cattttttgc gatccgcctc tccatctcct gcgcgactgt cctccccttc
     1441 atatcgatcc cctgcgcctt ggccgcttgc gccagcaccc gctcaatctc cgggttatct
     1501 ctgaccctct gagcaagccc cttcatctcc cgctcgatcc gcagccggtc gccatagctg
     1561 tcggccttgc cacgccgcgc ccccaggtcg cgccaggacg tcatcagagc cggccccagg
     1621 gatttggaca ggttgataag tggatgactg cttcgatttg aacccgtagg atgggtgaag
     1681 gagcagacga tatgagcaga cgaccgaggc ggaaccacac agccgctttc aaa
//
LOCUS       DATMTI010001112         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_635894, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001112 DATMTI010000000
VERSION     DATMTI010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635894"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2178
                     /locus_tag="VN039_17555"
     CDS             <1..>2178
                     /locus_tag="VN039_17555"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HWV47803.1"
                     /translation="IHLQQTDGTYKRLDFSTGQSTSVGSGDFNGDGIPDVAFGLSYGN
                     LRVYLGPVTSTSKSIDISAGYDHWDMDVADLNGDGFDDIVVAAVGFNQVYFIYGSSSF
                     QTKRVVRSMALSLSTSGLQQIISAGDVDGDGQMELAVAGPTRLAVLEPVKDGTGNYRL
                     LKSLPLPNSGSPESVSIKDVFNDGKAEVMVVNKTSRLFVYDTDSLYGSDAPRQLEIGG
                     SGDDTLTVADFDRDGIQDFVLFYASPKRGIPDNLAVHFGTGEHSGNFITPPTEFSGIV
                     RGNDGTYRRTYKDGTVAVFNPEGFQTAVIDSNGNTTTYDYAEGRLVTITDPAGLKVEM
                     SYANERLARAVYSDGRVSTFEYDQTGNLLGVTQPNLSLPQTVSLPNGEQVHLAAEGSG
                     SGSGSTYAYQEASVTVGNTKISYSYDANGKMIASIDERGKAVTYNYTAAGRLQSSTLP
                     DASSVSVDIAKTLGLATFGVDLGAPSATKHVAPEERTVKLTDAKGNKAEQEVNEWGAV
                     VRTKDPLGRETIFTRNANNLVTRIEAPSDASAGNRLVTELVYDSRGNVTSKREAVGTP
                     LERTVSYVYEPKFNRVVRMVDAAGKATQYQYDAKGNLTLQTNSDGTSQAFTYDARGLR
                     LTMRDERANVTTNAYDDYGRLISVTDPKGTVTSYTRDAAGNPSVTVRGNGTPEQQIEL
                     AFFDSKNRRIFSIDGEGGQTQIVYDAAGNVAKTVDATKISVSN"
BASE COUNT          491 a          621 c          606 g          460 t
ORIGIN      
        1 gaatccatct ccagcagacc gacggcacct acaagcgtct cgatttctcg accggacagt
       61 cgacgtccgt tggttccggc gattttaatg gtgatggaat tcccgatgtt gctttcggcc
      121 tcagctacgg aaatctccgg gtctatctcg gtccggtgac atcgacctca aagtcgatcg
      181 acatttccgc gggctacgac cattgggaca tggatgtggc cgatttgaac ggcgacggtt
      241 tcgacgacat cgtcgtggcg gccgtcggtt tcaaccaagt ttatttcatc tatggctcca
      301 gcagcttcca gacgaagcgc gtcgtcagaa gcatggcgct ttcattgtca acctccgggc
      361 ttcagcaaat catttccgcc ggtgacgtgg atggtgacgg gcagatggag ttggccgtcg
      421 cgggaccgac acgattggcc gtgctcgagc ccgtaaaaga tggaacgggc aactaccgtc
      481 tcctcaagtc tcttcccttg ccaaactctg gaagtccgga atccgtctcg atcaaagacg
      541 tcttcaacga cggcaaggcg gaggtcatgg ttgtcaacaa gacatcacgc ctgttcgtct
      601 acgatacgga ctcgctttat ggcagcgacg cgccgcgtca gcttgaaata ggcggttccg
      661 gcgacgacac attgacggtg gccgatttcg accgcgatgg aatccaggat tttgtcctct
      721 tctacgcgag ccccaaaagg ggaatcccgg acaatctcgc cgttcacttc ggaacgggtg
      781 agcattcggg caatttcata accccgccga cggagttttc aggcatcgtc cgtggcaatg
      841 acggaaccta ccgccgtact tataaggacg gtactgtagc cgtcttcaac cccgagggtt
      901 tccagaccgc ggttatagac agtaacggca ataccaccac ctatgactat gccgagggcc
      961 gcctcgtcac gattaccgat cctgccggtc tgaaggtgga gatgagctat gccaacgagc
     1021 ggttggcgcg ggccgtctat tcggatggcc gtgtctcgac tttcgagtat gaccagaccg
     1081 gaaatcttct cggcgtcacc cagcccaatc tttccttgcc gcaaactgtt tccctgccga
     1141 acggtgaaca ggttcacctt gcggcggaag gctcgggcag cggcagtgga tcgacctatg
     1201 cctatcagga ggctagcgtc actgtcggca atacgaagat cagctatagt tatgatgcca
     1261 atggcaaaat gattgcctcc atcgatgaac ggggcaaggc agtcacctac aactacaccg
     1321 cggccgggcg gctgcaatcc agtactctgc ctgatgcttc aagtgtttcg gttgatattg
     1381 ccaagaccct cggtctcgcc actttcggtg tcgatcttgg tgctccctcc gcaacgaaac
     1441 acgttgcgcc ggaggagcgc accgtgaaac tcaccgacgc gaagggaaat aaggccgagc
     1501 aggaggtgaa tgaatggggc gccgtggttc ggaccaagga tccgctgggc cgcgagacca
     1561 ttttcacgcg taacgccaac aatcttgtaa cgaggatcga ggcgccaagc gacgcttccg
     1621 ctggcaacag gcttgttacc gaacttgttt atgacagccg tggcaacgtc acttcgaagc
     1681 gtgaggcagt cggcacgccg ctggagcgga cggtatccta tgtatacgaa ccaaagttca
     1741 accgcgtcgt ccgcatggtc gatgctgcgg gtaaagccac gcaatatcaa tatgatgcca
     1801 aaggcaatct gacccttcag accaattccg acggtacgtc ccaagcattc acctatgacg
     1861 cgcgtggtct gcgcctgacc atgcgggacg aaagagcgaa tgtcaccacc aatgcatatg
     1921 atgactacgg acggttgatc tccgtcactg atcccaaggg aacggtgacc agctataccc
     1981 gggatgccgc cggcaatcca tccgtcacgg ttcggggcaa tggaacgcct gagcagcaga
     2041 ttgagcttgc tttctttgac agcaaaaacc gacggatttt ctccatcgac ggggagggag
     2101 gccaaaccca gatcgtctat gatgcggctg gcaacgtcgc caaaaccgtt gatgcaacga
     2161 agatctccgt ttcgaata
//
LOCUS       DATMTI010001113         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_593366, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001113 DATMTI010000000
VERSION     DATMTI010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593366"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(603..1862)
                     /locus_tag="VN039_17560"
     CDS             complement(603..1862)
                     /locus_tag="VN039_17560"
                     /inference="COORDINATES: protein motif:HMM:NF041640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HWV47804.1"
                     /translation="MNAKHVFKLWVMGLCGVATLAFTPVLDITPAFAHGERSQEPFLR
                     MRTVNWYDTEWVGKSTKVNDVTELRGKFHLSEDWPRAVVKPNRTFLNIGSPSSVFVRL
                     SSKINGTPMFVAGPMEIGRDYEYVVVLKARLPGHHHIHPMFAVKDAGPIAGPGGWMDI
                     TGRYEDFTNPIKTLTGETFDSETMGTATGIMWHLFWAAVAIFWVGFFMIRPMYLVRAR
                     VLAAYGDEILLDPIDRKVGTAMLIFVLVVVTVGYLAADARHPVSVPLQAGETKVKPMP
                     IKPNPFVVEVTHAEYDVPGRALRMTLHATNNGTEPVTIGEFTTAGIRFTNKMGATKLD
                     PNYPAELVAQAGLTMDNEAAIQPGQTVDIKIESKDVLWEVQRLVDILHDPDQRFAGLL
                     MSWTDKGERLINPIWAPVLPVFTRLGA"
     gene            complement(1859..2701)
                     /locus_tag="VN039_17565"
     CDS             complement(1859..2701)
                     /locus_tag="VN039_17565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit A"
                     /protein_id="HWV47805.1"
                     /translation="MFRTDEIIKASKLPPEGVAMSRHIDYIYFIPILFVTIVGTFHMH
                     FDLLAGDWDFWLDWKDRQWWPIVTPITAITFCAALQYYNWVNYRQPFGATITILALLA
                     GKWVTIVAAWWWWSNYPYNFVMPATLLPSAIVLDIVLLLTRNWTLTAVIGAWMFAALF
                     YPTNWAIFAYSHTPLVVDGTLLSWADYMGFAYVRTGTPEYIRMIEVGSLRTFGGHSTM
                     ISSFFAAFASSLMYILWWQFGKFFCTSYFYLTDDRQKTTKVYDVFAYATLANQDKAKI
                     GGKA"
     gene            complement(2820..>3100)
                     /locus_tag="VN039_17570"
     CDS             complement(2820..>3100)
                     /locus_tag="VN039_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit C"
                     /protein_id="HWV47806.1"
                     /translation="VAAVVGPMFILPNVGLNEWGHAFWFVDELFSAPLHWGFVTLGWC
                     GLFGAAGGVAAQIVSRMSNLADVIWNNAPKSILDPFPSQVGPGHKTVY"
BASE COUNT          735 a          911 c          856 g          598 t
ORIGIN      
        1 tccaacaagg aactgaccgc cacgcagaaa aaggtgaagg cggcaattcc gtagtcccgc
       61 attcgatggg tattgagcgc atccgcccga ggaaaatgcc gaagggcaat ccgctcaaca
      121 gtgtcgaata acggtgaggg aggagaagta ctcgaagagg tatccatcga tctaaccgag
      181 tgtgtcacgc aggagcctgt gtgttgaacc ctccgtagaa acaacaggat ttacgaatcc
      241 accagtctcg taagagcggt aacatcatac aggacccttg tcagacgggc aagttgtggc
      301 aagacgacga gcctattcct caggatttgt aaagatgaga tcaaccgagg ctcagagcat
      361 ttcgcgtgat tcgttgtgca caactagtgg gcactggtga cgaagatgcg gtatgtatag
      421 ctaggacaca ctcgaacggt cactgtccac tggcgctagg ctgttccatg agcgacgcac
      481 ggggaggaga cgggcatgca cagggtgaaa gcagagaatc ggggagagga aaaaacaaaa
      541 acgccgaccc gcctcgccat ttccatgggg gagggccggc gtcttgttag aagagcgttg
      601 ccctaggcac ccagtcttgt gaagaccggg agaaccggag cccagattgg attgatgagc
      661 cgctcaccct tgtctgtcca ggacatcaac aatcctgcga agcgctgatc cggatcgtgg
      721 agaatgtcca ccaaccgctg cacttcccac agaacgtctt tagactcgat cttgatgtct
      781 acagtctgac caggctggat cgcggcttca ttgtccatcg tcaagccagc ttgtgcaacc
      841 agctcggccg gatagtttgg atcgagtttg gttgccccca tcttgttggt aaaccggatg
      901 cctgcagtcg tgaattcacc gatggtcaca ggctcggtgc cgttattggt cgcatgaaga
      961 gtcatgcgca aggcccgacc tggaacgtcg tattcagcat gggtgacttc aaccacgaac
     1021 gggtttggct tgatcggcat cggcttgacc tttgtttcac cggcctgaag cggtacgctt
     1081 acaggatggc gcgcatccgc cgccagatac ccgacggtca cgacgaccaa cacgaagatc
     1141 agcatcgcgg ttccgacttt gcggtcgata gggtcgagca agatctcgtc cccatatgcc
     1201 gccaacaccc gtgcccggac caggtacatc ggtcggatca tgaagaagcc gacccagaag
     1261 atggcaactg ccgcccagaa caggtgccac ataataccgg tcgcagtacc catcgtttct
     1321 gagtcgaacg tttcaccggt cagtgtcttg atcggattgg tgaagtcctc atatcgacca
     1381 gtgatgtcca tccatccacc gggtcctgcg ataggacctg catccttgac ggcaaacatt
     1441 ggatggatgt gatgatgacc ggggagtcga gccttcaaga cgaccacata ctcgtaatcc
     1501 cgtccaattt ccatcggacc agcaacgaac atcggcgtcc cgtttatttt tgagctaaga
     1561 cgcacgaaca ctgaactggg ggaaccgatg ttgaggaagg tacggtttgg tttcactacc
     1621 gcacgaggcc aatcctcgga taaatgaaac ttgcccctca gctcggtcac atcgttgacc
     1681 ttggtcgact ttcctaccca ttcagtatcg taccaattca cggtgcgcat ccgcaggaac
     1741 ggttcctgcg aacgttcgcc gtgagcgaat gcaggggtga tatcgagaac cggcgtgaac
     1801 gccagcgtcg ccaccccgca gaggcccatt acccagagtt taaagacgtg tttggcgttc
     1861 atgctttccc cccgattttg gctttgtcct ggttcgccag ggttgcatag gcgaacacat
     1921 cgtacacctt cgtggtcttc tgtcggtcat ccgtcaggta gaagtaggag gtacagaaga
     1981 acttcccgaa ctgccaccac aggatgtaca tcaacgacga ggcgaaggcg gcgaagaacg
     2041 aggaaatcat cgtgctgtgc ccgccgaagg tccgcagcga gcccacttcg atcatccgaa
     2101 tatactccgg cgtcccggtc cggacatacg caaagcccat gtagtccgcc caggagagca
     2161 aggtgccatc gacgacgagc ggcgtatggc tataggcaaa gatggcccag ttggtcggat
     2221 aaaagagggc cgcaaacatc caggccccga tcaccgccgt cagcgtccag ttcctggtca
     2281 acaacagcac aatgtccagg acaatggcgc tcggcagcag cgtggccggc atgacgaagt
     2341 tgtacggata gttcgaccac caccaccagg cggcgacgat cgtgacccac ttccccgcga
     2401 gcagcgcgag gatggtgatc gtggccccaa agggctggcg atagttcacc cagttgtagt
     2461 actgcagggc ggcgcaaaag gtaatggccg tgatcggcgt cacgatcggc caccactgcc
     2521 gatccttcca atccaaccag aagtcccagt cgcctgccaa caagtcgaag tgcatgtgga
     2581 acgtgcccac gatggtgacg aacaaaatcg gaatgaaata aatgtagtcg atgtgccggg
     2641 acatcgcgac gccttccggc ggcagcttcg aggccttaat gatctcatcg gttctaaaca
     2701 taactgacat cctccgtcat tccagccggc ggagaccggc ccggcgctcc gtgccgcgaa
     2761 gcgccgaacc cagcctgcca tgccgacctc tagttatgtg gactgcttgc cattcccgtt
     2821 tagtagacgg tcttatgccc gggacccacc tggctgggga acggatccag gatgctcttc
     2881 ggcgcgttgt tccaaatcac gtccgccaga ttcgacatcc ggctcacgat ctgcgccgcc
     2941 accccgcccg cggctccgaa caacccgcac caccccaacg tcacgaagcc ccagtgcaac
     3001 ggcgccgaga acagctcatc cacgaaccag aacgcatggc cccactcatt gagtcccacg
     3061 ttcggcagaa tgaacatcgg ccccaccacc gccgccacca
//
LOCUS       DATMTI010001114         5566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U13008 k141_126740, whole
            genome shotgun sequence.
ACCESSION   DATMTI010001114 DATMTI010000000
VERSION     DATMTI010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943609
            Sequence Read Archive: SRR7448277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:12:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,517
            CDSs (total)                      :: 3,426
            Genes (coding)                    :: 3,407
            CDSs (with protein)               :: 3,407
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 17, 21 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 7 (5S, 16S)
            partial rRNAs                     :: 10, 21 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 2 of 21
            Pseudo Genes (incomplete)         :: 16 of 21
            Pseudo Genes (internal stop)      :: 4 of 21
            Pseudo Genes (multiple problems)  :: 3 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126740"
                     /isolate="SMAG_U13008"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..2631)
                     /gene="topA"
                     /locus_tag="VN039_17575"
     CDS             complement(292..2631)
                     /gene="topA"
                     /locus_tag="VN039_17575"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWV47807.1"
                     /translation="MGKSLIIVESPTKARTITKYLGRGYTVMASVGHVKDLPTSKLGV
                     DLENDFEPQYVTIKGKSKVLAEIKKKAEEADKVFLAPDPDREGEAIAWHIEQELLGKS
                     KKKKKDGKIFRVLFNEITESAIKRALQSPGEIDMKLVNAQQARRVLDRIVGYQGSQLL
                     WNKVRRGLSMGRVQSVAMRLICEREAEREAFRSEEYWSITALLAGTNPPAFEAKLHSI
                     NGEEASIENAEQAGRIVGEIQGKAFVVQSIERREKKRNPVAPFITSRLQQDASRKLHF
                     SPKKTMTLAQQLYEGIEIGAEGATGLITYMRTDSPRISNEAMADAREVIQSRFGADYL
                     PATANVYKTSKAAQEAHEAIRPTSAGRDPESVRQYLDHDQYNLYKLIWNRFIASQMVP
                     AILDVTRIDSAPVGTKENYLFRSTGTVVKFPGHTIVYMEGIDKEALSQKPKADQEADD
                     DERQLPALSEGERLRLVEQEGQTVLGMLSKQHFTQPPPRYNEALLIKELEEKGIGRPS
                     TYAAIISTIQDRKYVEKTEGRLVPTETGKTVNDFLLKGFPKLINVDFTSQLEEQLDEV
                     EEGNKQWVAAVRDFYMPFTREMERAKTIPGPKDIVEPPTDIPCEKCGRMMEIKWGRNG
                     KFLACPAYKDDPPCKNTQNFEKLPDGTIKIVPKQELTTDQVCDKCGSPMVVKTGRFGK
                     FIACSGYPQCKTTKPLALGVKCPQPDCGGDLVQKRTRKGRSFFACSNYPKCEFALWDR
                     PVPKACPTCQAPFLVEKVSKQDGRSVQCRNQDCGYREAG"
     gene            complement(2886..4004)
                     /gene="dprA"
                     /locus_tag="VN039_17580"
     CDS             complement(2886..4004)
                     /gene="dprA"
                     /locus_tag="VN039_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWV47808.1"
                     /translation="MDDASLTCWLTLQAIDGVGDRTLLKLIHAFGTPHAVLGATMDDL
                     IGAGCSPELAESVRRGPESSIRRQIDRQVKIVERFKIQTVTLFDRSYPARLKTIPDPP
                     PLLYVSGSLSPKDEVAVAIVGGRRATPSGRLITEEIAKDLAGCGVTIVSGLARGIDAA
                     AHRGALTGKGRTIAVLGCGIDRTYPSEHHMLRRNIESHGVVISELPIGAAPQSHHFPR
                     RNRIISGLSLGVLVGEAATDSGSLITAKLALEQGREVFAVPGSVKEEACRGSNRLIKE
                     GAKLVEGAQDILDEILPQVDARQRATLHFDRALTEVRVPLGKEEASVYETLSYEARSI
                     DTVIEHTGLSAAEVSAALLSLELNGRIRQLPGQQYIRL"
     gene            complement(4090..>5566)
                     /locus_tag="VN039_17585"
     CDS             complement(4090..>5566)
                     /locus_tag="VN039_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWV47809.1"
                     /translation="EETRVAVLDGGVVTDLFGDRTKHKDFVGNIYKGKVAKVLPGMQA
                     AFVDIGLEKAAFMHVSDLLVDAEPGDMLVEAEEDEKDSEMLRPKHQSAKPIEQLLSEG
                     QELMVQISKGPIGTKGSRVTTYVSLPGRYLVFMPNVDHIGVSRRIPRDEERARLKEIM
                     RRVRRSGFGYIVRTVSEGVKEDELKSDVDFLHVLWQDILTKREQMPAPALLHSDLSLS
                     FRVVRDLFGKKVDRLWIDSREEYEAIRDFVQRFSPEQTSRIHFYDKDEPLFDHLGVEQ
                     EIARALSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETILRNNLEAAKEVA
                     YQLKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMASDKARTRISRISDLGLIEISR
                     ERVREDLLRSLSEPCRYCDGRGYTKSPTTVAYEIFREIRRIEPAADQQRIIVGAHPTV
                     AELLQDEERYGVEVLERDCSAKIIVTPDSQLHLEQYDLVVL"
BASE COUNT         1110 a         1725 c         1440 g         1291 t
ORIGIN      
        1 caatgactct cctcacacac acttctttaa caacaacgga cgcgtatatg caactggttc
       61 tcctcacgtc gcagccgtac ggacctccgt cgcatccggt tgtccacgcc agtagtgccg
      121 tccctcaact gagccgcacc agtcacccat cagatcacga gatcacgcag atcccagcgt
      181 gggagatgcg cgtcagctgg ctgcgttcga gatgtgactg aagggggact cttgcgctca
      241 gtgcccattg aatgtggtga ttgatgggat gcaaggaaca gaagaaaatc gttacccggc
      301 ttcgcggtag ccacaatcct gattgcggca ttggacgctt cggccgtctt gcttgctgac
      361 tttctctacc aagaacgggg cctggcacgt cgggcacgct ttgggcaccg ggcggtccca
      421 gagagcaaac tcacacttgg ggtaattact gcacgcgaaa aatgagcggc ctttgcgcgt
      481 ccgcttctgc acgaggtcgc cgccgcaatc gggttgcgga catttgacgc cgagcgccag
      541 cggtttggtc gtcttacact gaggatagcc agaacaagcg atgaacttgc cgaaacggcc
      601 ggtcttcacc accatggggc ttccgcactt gtcgcaaacc tgatcggtgg tcaattcctg
      661 tttaggcacg attttgatgg tgccgtccgg cagcttttcg aagttttgcg tgttcttgca
      721 tgggggatcg tccttgtacg ctgggcaagc gagaaacttc ccgttccggc cccacttgat
      781 ctccatcatc cgtccgcact tctcgcacgg aatgtcggtc ggaggttcga caatatcttt
      841 gggaccggga atcgtcttcg cccgctccat ttcccgcgta aacggcatgt aaaaatcccg
      901 caccgctgcg acccactgtt tattcccctc ttccacttcg tccagctgtt cttccagctg
      961 agacgtgaag tcgacattga tgagtttagg aaagcccttc agtaggaaat cgttgacggt
     1021 tttcccggtc tccgtaggga ccaagcgtcc ttcggtcttt tccacgtact tgcgatcctg
     1081 gatcgtggag ataatggcgg cgtaggtcga cggtcgcccg atgccttttt cctccagttc
     1141 tttgatcaag agcgcttcgt tataccgagg cggcggctgg gtgaaatgtt gctttgacaa
     1201 catgcccagg accgtctgcc cttcctgctc gaccagccgc agccgttccc cttcggatag
     1261 ggccggtagc tgacgttcat cgtcatcggc ttcctgatcg gccttcggct tctgcgacag
     1321 cgcttcctta tcgatcccct ccatgtagac aatcgtatga ccggggaact tcaccacagt
     1381 tccagtggaa cggaagagat agttttcttt ggttccaacc ggtgccgaat cgatacgggt
     1441 aacgtccaaa atggccggca ccatctgcga tgcgatgaac cgattccaga tcagcttata
     1501 aagattgtac tgatcgtgat ccaagtactg gcggaccgat tccggatcgc gtcccgctga
     1561 ggtcggcctg atggcttcat gcgcttcctg ggccgccttc gaggtcttgt agacattcgc
     1621 cgtcgcaggg agatagtccg ccccaaatcg agactggatc acctcgcgcg cgtcggccat
     1681 cgcctcattg gaaatgcgcg gggaatcggt tctcatatag gtgatgagac cggtcgcgcc
     1741 ctcggcgccg atttcgatcc cttcatacaa ctgctgcgcc agagtcatcg ttttcttcgg
     1801 tgagaagtgc agcttccgcg aagcgtcctg ctgcagccga ctcgtaatga acggcgcgac
     1861 gggattccgt ttcttttctc ggcgttcgat cgattgtacg acgaacgcct ttccctgaat
     1921 ctcgccgacg atgcggccgg cttgttcggc gttttcaatc gaggcctctt cgccgttgat
     1981 actgtggagc ttcgcttcga atgccggggg attggttccc gccagcagcg cggtgatcga
     2041 ccagtactct tcgcttcgga acgcttcccg ttccgcttcc cgctcgcaaa tcagccgcat
     2101 ggccaccgac tgcactcgcc ccatgctgag cccgcgccgg accttattcc agagcaactg
     2161 actgccttga taccccacga tgcgatccaa cacacggcgg gcctgctggg cattcaccag
     2221 cttcatatcg atctcgcccg gcgactggag cgcgcgcttg atggctgatt ccgtgatttc
     2281 gttgaagagg acccggaaga tctttccgtc tttctttttc ttcttcgatt ttccgagcag
     2341 ctcttgctca atgtgccagg caatcgcctc cccttcacga tcggggtccg gcgccaaaaa
     2401 gaccttgtcg gcttcctcgg ccttcttctt gatctcggcc aacaccttgg acttgccttt
     2461 gatggtgacg tactgcggtt cgaaatcgtt ctcgagatcc acgcccaatt tgctggtcgg
     2521 aagatccttg acatgcccca ccgacgccat gacggtatag ccgcggccca gatatttcgt
     2581 aatcgtcctc gccttggtcg gcgactcaac aataatcaac gattttccca tgacaactcc
     2641 gtctaacctc tcaaggattg tatcatccgt accaaatatc ggagattcgt gcaactagaa
     2701 tggcagtcgg caaccatcag tgagaggtta ggaaccgtaa aggatgctat gaaagtgtaa
     2761 cggaggcaag gcctttcggc tatgcgcttc acgtattatt ccactcgtca cacgcgaaga
     2821 cgttcctcac accgacgagt cgtcgatgtt cactcgacga cgattgacgc gatgtcttgc
     2881 cggtgctaga gacggatgta ttgttgccca ggaagttggc gaatccgtcc gttcaattcc
     2941 aatgaaagca acgccgcgga cacttccgca gcgctcagcc cggtgtgctc aatcaccgta
     3001 tcgatggatc gggcttcata ggatagcgtt tcgtataccg atgcctcttc tttccctagc
     3061 ggcacgcgca cttccgtcaa cgcgcggtcg aaatgcagcg tggcccgctg ccgggcatcg
     3121 acttgcggaa gaatctcatc gaggatgtct tgtgctcctt cgaccaattt cgccccctcc
     3181 ttgatcaggc gattcgatcc gcgacaggcc tcttccttga ctgagcctgg tacggcaaat
     3241 acctcccggc cttgctccaa cgccagtttc gcagtgatca acgagccgct gtcggttgcc
     3301 gcctcgccga ccagcacgcc caaggaaagt ccgctgatga tccgatttct tcgcgggaag
     3361 tgatgacttt gaggggcggc gccgatcggc agctccgaga tcacgacgcc gtgtgattca
     3421 atattccgcc gcagcatgtg atgctccgag gggtaggtcc gatcgatgcc gcaaccgagg
     3481 accgcgatcg tgcgcccctt acccgtcagc gcccctcggt gcgccgcagc atcgattccc
     3541 cgagccaagc cgctcacgat cgtcacccca catccggcca ggtccttcgc aatctcctcc
     3601 gtgatgagtc ttccggacgg agtcgcccgt cgtccaccca caatcgccac cgcgacctcg
     3661 tccttcggtg ataggctccc gctcacatac aacaatggcg gcggatcggg aatggttttg
     3721 agtcgggctg gataggatcg atcaaacaag gtgactgttt ggatcttgaa gcgttcaaca
     3781 atcttcacct ggcggtcgat ctgtcgccgg atactcgact caggcccgcg ccgtacggat
     3841 tcggccaatt cgggactgca accggcaccg atcaagtcgt ccatcgttgc accaagcact
     3901 gcatggggag ttccaaacgc gtgaatcaac ttgagaaggg tacgatcgcc gacaccgtca
     3961 atcgcttgga gcgtgagcca gcaggtcaag gatgcatcat ccatgatcgg tctcgatcat
     4021 cggcagggga ctcgcttcaa cagcaggaag gtccgctagg cagaacatct cctcgacaac
     4081 gagtggcggt tagagcacga ccagatcgta ctgctcaaga tgcaactgac tgtccggtgt
     4141 gacgatgatt ttcgccgaac agtctcgctc caatacttcc actccgtaac gctcttcgtc
     4201 ttgcagcaat tcagccaccg tcggatgggc tccaacgata atccgttgct gatcggcagc
     4261 cggttcaatt cgtcggattt cgcggaaaat ttcgtacgcc acggtcgtgg gagacttcgt
     4321 atatccacgg ccgtcgcagt agcggcacgg ctccgacagc gaacgtaaca ggtcctcccg
     4381 cacccgttcg cgggagattt cgatcaagcc gaggtccgaa atcctcgata tcctcgttct
     4441 cgccttgtcc gacgccatgg cgtccaccaa cgcatggtag accttatccc gattcttctc
     4501 ccgctccatg tcgatgaagt cgacaataat gatgccgccg attccccgca acttcagctg
     4561 ataggcgacc tctttggccg cctcgaggtt gttgcgcaaa atggtttctt cctgatcccg
     4621 tttccccaca aaacgtcccg tattgacgtc gatcaccgtc atcgcttcgg tatgatcgat
     4681 cacgagatag cctcccgact tgagccagac tttccggctc aatgcacgcg cgatttcctg
     4741 ctccaccccc agatgatcga acaagggctc atccttatcg tagaaatgaa tccgcgaggt
     4801 ctgttcggga gaaaatcgct gcacgaaatc ccgaatggct tcgtattcct cccgcgaatc
     4861 gatccacaat cgatccacct ttttgccgaa caggtccctc acgacacgga agctcaagct
     4921 caagtcagaa tgcagcaacg cgggggcggg catttgttcc cgcttcgtca agatgtcctg
     4981 ccagaggacg tgcagaaagt cgacgtcgga tttcaactca tcttccttga cgccttcact
     5041 gaccgtccgc acgatatagc cgaagccgga acgcctgacc cggcgcatga tctccttcag
     5101 cctggcccgt tcttcatccc gcggaatccg ccgagacaca ccgatatgat cgacgttggg
     5161 cataaagacg agatatcgcc ccggaagcga gacgtacgtc gtcactctgg accccttggt
     5221 gccgatcggc cccttcgaaa tctggaccat cagctcctgg ccctcactca gcaactgctc
     5281 gatcggtttc gcgctctgat gctttggccg gagcatttcc gaatcttttt catcttcctc
     5341 ggcctccacc aacatgtctc ccggctctgc atccaccagc agatcggaga cgtgcataaa
     5401 cgccgctttt tccaagccga tgtccacaaa cgcggcttgc atgcccggca ataccttggc
     5461 cacctttcct ttatagatat ttccgacaaa atccttatgc ttcgtgcggt cgccgaacaa
     5521 atcggtgacg actccgccgt cgagcacggc cacacgggtt tcctcc
//
